LOCUS       DAZGLJ010000001        85969 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000001 DAZGLJ010000000
VERSION     DAZGLJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 85969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.001"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            161..1561
                     /gene="xanQ"
                     /locus_tag="ACHK49_000001"
     CDS             161..1561
                     /gene="xanQ"
                     /locus_tag="ACHK49_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO9260709.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            1579..2895
                     /gene="guaD"
                     /locus_tag="ACHK49_000002"
     CDS             1579..2895
                     /gene="guaD"
                     /locus_tag="ACHK49_000002"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO9260710.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWARLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            2931..4298
                     /gene="ghxQ"
                     /locus_tag="ACHK49_000003"
                     /gene_synonym="ygfQ"
     CDS             2931..4298
                     /gene="ghxQ"
                     /locus_tag="ACHK49_000003"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO9260711.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(4334..4822)
                     /gene="ygfS"
                     /locus_tag="ACHK49_000004"
     CDS             complement(4334..4822)
                     /gene="ygfS"
                     /locus_tag="ACHK49_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO9260712.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(4822..6741)
                     /gene="ygfT"
                     /locus_tag="ACHK49_000005"
     CDS             complement(4822..6741)
                     /gene="ygfT"
                     /locus_tag="ACHK49_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO9260713.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            7177..8625
                     /gene="uacT"
                     /locus_tag="ACHK49_000006"
     CDS             7177..8625
                     /gene="uacT"
                     /locus_tag="ACHK49_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO9260714.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            8627..8752
                     /gene="yqfG"
                     /locus_tag="ACHK49_000007"
     CDS             8627..8752
                     /gene="yqfG"
                     /locus_tag="ACHK49_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO9260715.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(8749..8820)
                     /locus_tag="ACHK49_000008"
     CDS             complement(8749..8820)
                     /locus_tag="ACHK49_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9260716.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            8875..9423
                     /gene="idi"
                     /locus_tag="ACHK49_000009"
     CDS             8875..9423
                     /gene="idi"
                     /locus_tag="ACHK49_000009"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO9260717.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHRAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYGLGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(9466..10983)
                     /gene="lysS"
                     /locus_tag="ACHK49_000010"
     CDS             complement(9466..10983)
                     /gene="lysS"
                     /locus_tag="ACHK49_000010"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO9260718.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGVAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(10993..12091)
                     /gene="prfB"
                     /locus_tag="ACHK49_000011"
     CDS             complement(join(10993..12015,12017..12091))
                     /gene="prfB"
                     /locus_tag="ACHK49_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO9260719.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(12182..13915)
                     /gene="recJ"
                     /locus_tag="ACHK49_000012"
     CDS             complement(12182..13915)
                     /gene="recJ"
                     /locus_tag="ACHK49_000012"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO9260720.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVVHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(13921..14631)
                     /gene="dsbC"
                     /locus_tag="ACHK49_000013"
     CDS             complement(13921..14631)
                     /gene="dsbC"
                     /locus_tag="ACHK49_000013"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO9260721.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(14656..15552)
                     /gene="xerD"
                     /locus_tag="ACHK49_000014"
     CDS             complement(14656..15552)
                     /gene="xerD"
                     /locus_tag="ACHK49_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO9260722.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLRAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            15664..16185
                     /gene="fldB"
                     /locus_tag="ACHK49_000015"
     CDS             15664..16185
                     /gene="fldB"
                     /locus_tag="ACHK49_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO9260723.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(16225..16632)
                     /gene="ygfX"
                     /locus_tag="ACHK49_000016"
     CDS             complement(16225..16632)
                     /gene="ygfX"
                     /locus_tag="ACHK49_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO9260724.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(16613..16879)
                     /gene="sdhE"
                     /locus_tag="ACHK49_000017"
     CDS             complement(16613..16879)
                     /gene="sdhE"
                     /locus_tag="ACHK49_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO9260725.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            17122..18102
                     /gene="ygfZ"
                     /locus_tag="ACHK49_000018"
     CDS             17122..18102
                     /gene="ygfZ"
                     /locus_tag="ACHK49_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO9260726.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(18298..18957)
                     /gene="yqfA"
                     /locus_tag="ACHK49_000019"
     CDS             complement(18298..18957)
                     /gene="yqfA"
                     /locus_tag="ACHK49_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO9260727.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(19121..19432)
                     /gene="yqfB"
                     /locus_tag="ACHK49_000020"
     CDS             complement(19121..19432)
                     /gene="yqfB"
                     /locus_tag="ACHK49_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO9260728.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            19477..20910
                     /gene="bglA"
                     /locus_tag="ACHK49_000021"
     CDS             19477..20910
                     /gene="bglA"
                     /locus_tag="ACHK49_000021"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO9260729.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRIHPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            complement(20967..21710)
                     /gene="ygfF"
                     /locus_tag="ACHK49_000022"
     CDS             complement(20967..21710)
                     /gene="ygfF"
                     /locus_tag="ACHK49_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9260730.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYTASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(21971..24844)
                     /gene="gcvP"
                     /locus_tag="ACHK49_000023"
     CDS             complement(21971..24844)
                     /gene="gcvP"
                     /locus_tag="ACHK49_000023"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO9260731.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(24963..25352)
                     /gene="gcvH"
                     /locus_tag="ACHK49_000024"
     CDS             complement(24963..25352)
                     /gene="gcvH"
                     /locus_tag="ACHK49_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO9260732.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(25376..26470)
                     /gene="gcvT"
                     /locus_tag="ACHK49_000025"
     CDS             complement(25376..26470)
                     /gene="gcvT"
                     /locus_tag="ACHK49_000025"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO9260733.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            26761..26844
                     /locus_tag="ACHK49_000026"
     CDS             26761..26844
                     /locus_tag="ACHK49_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9260734.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            complement(26918..28120)
                     /gene="ubiI"
                     /locus_tag="ACHK49_000027"
                     /gene_synonym="visC"
     CDS             complement(26918..28120)
                     /gene="ubiI"
                     /locus_tag="ACHK49_000027"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO9260735.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(28143..29321)
                     /gene="ubiH"
                     /locus_tag="ACHK49_000028"
                     /gene_synonym="visB"
     CDS             complement(28143..29321)
                     /gene="ubiH"
                     /locus_tag="ACHK49_000028"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO9260736.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(29318..30643)
                     /gene="pepP"
                     /locus_tag="ACHK49_000029"
     CDS             complement(29318..30643)
                     /gene="pepP"
                     /locus_tag="ACHK49_000029"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO9260737.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(30669..31247)
                     /gene="ygfB"
                     /locus_tag="ACHK49_000030"
     CDS             complement(30669..31247)
                     /gene="ygfB"
                     /locus_tag="ACHK49_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO9260738.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            31415..31744
                     /gene="zapA"
                     /locus_tag="ACHK49_000031"
     CDS             31415..31744
                     /gene="zapA"
                     /locus_tag="ACHK49_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO9260739.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            31786..31969
                     /gene="ssrS"
                     /locus_tag="ACHK49_000032"
     ncRNA           31786..31969
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHK49_000032"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            31990..32592
                     /gene="fau"
                     /locus_tag="ACHK49_000033"
     CDS             31990..32592
                     /gene="fau"
                     /locus_tag="ACHK49_000033"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO9260740.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(32693..32752)
                     /locus_tag="ACHK49_000034"
     CDS             complement(32693..32752)
                     /locus_tag="ACHK49_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO9260741.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(33085..33144)
                     /gene="ibsC"
                     /locus_tag="ACHK49_000035"
     CDS             complement(33085..33144)
                     /gene="ibsC"
                     /locus_tag="ACHK49_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO9260742.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(33373..34605)
                     /gene="serA"
                     /locus_tag="ACHK49_000036"
     CDS             complement(33373..34605)
                     /gene="serA"
                     /locus_tag="ACHK49_000036"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO9260743.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(34861..35520)
                     /gene="rpiA"
                     /locus_tag="ACHK49_000037"
     CDS             complement(34861..35520)
                     /gene="rpiA"
                     /locus_tag="ACHK49_000037"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO9260744.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(35576..35761)
                     /locus_tag="ACHK49_000038"
     CDS             complement(35576..35761)
                     /locus_tag="ACHK49_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9260745.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            35903..36796
                     /gene="argP"
                     /locus_tag="ACHK49_000039"
     CDS             35903..36796
                     /gene="argP"
                     /locus_tag="ACHK49_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO9260746.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            37000..39144
                     /gene="scpA"
                     /locus_tag="ACHK49_000040"
     CDS             37000..39144
                     /gene="scpA"
                     /locus_tag="ACHK49_000040"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO9260747.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            39137..40132
                     /gene="meaB"
                     /locus_tag="ACHK49_000041"
     CDS             39137..40132
                     /gene="meaB"
                     /locus_tag="ACHK49_000041"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO9260748.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            40143..40928
                     /gene="scpB"
                     /locus_tag="ACHK49_000042"
     CDS             40143..40928
                     /gene="scpB"
                     /locus_tag="ACHK49_000042"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO9260749.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAQLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            40952..42430
                     /gene="scpC"
                     /locus_tag="ACHK49_000043"
     CDS             40952..42430
                     /gene="scpC"
                     /locus_tag="ACHK49_000043"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO9260750.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(42427..43323)
                     /gene="ygfI"
                     /locus_tag="ACHK49_000044"
     CDS             complement(42427..43323)
                     /gene="ygfI"
                     /locus_tag="ACHK49_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9260751.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(43490..44230)
                     /gene="yggE"
                     /locus_tag="ACHK49_000045"
     CDS             complement(43490..44230)
                     /gene="yggE"
                     /locus_tag="ACHK49_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO9260752.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(44323..44958)
                     /gene="argO"
                     /locus_tag="ACHK49_000046"
     CDS             complement(44323..44958)
                     /gene="argO"
                     /locus_tag="ACHK49_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO9260753.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(45097..45957)
                     /gene="mscS"
                     /locus_tag="ACHK49_000047"
     CDS             complement(45097..45957)
                     /gene="mscS"
                     /locus_tag="ACHK49_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO9260754.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            46153..46251
                     /locus_tag="ACHK49_000048"
                     /pseudo
     CDS             46153..46251
                     /locus_tag="ACHK49_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(46315..47394)
                     /gene="fbaA"
                     /locus_tag="ACHK49_000049"
     CDS             complement(46315..47394)
                     /gene="fbaA"
                     /locus_tag="ACHK49_000049"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO9260755.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(47609..48772)
                     /gene="pgk"
                     /locus_tag="ACHK49_000050"
     CDS             complement(47609..48772)
                     /gene="pgk"
                     /locus_tag="ACHK49_000050"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO9260756.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(48822..49841)
                     /gene="epd"
                     /locus_tag="ACHK49_000051"
     CDS             complement(48822..49841)
                     /gene="epd"
                     /locus_tag="ACHK49_000051"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO9260757.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            50213..50644
                     /locus_tag="ACHK49_000052"
     CDS             50213..50644
                     /locus_tag="ACHK49_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO9260758.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            50667..51245
                     /locus_tag="ACHK49_000053"
     CDS             50667..51245
                     /locus_tag="ACHK49_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO9260759.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTNPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            51246..51953
                     /locus_tag="ACHK49_000054"
     CDS             51246..51953
                     /locus_tag="ACHK49_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO9260760.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATQ
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            51941..52618
                     /locus_tag="ACHK49_000055"
     CDS             51941..52618
                     /locus_tag="ACHK49_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9260761.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            52612..53289
                     /locus_tag="ACHK49_000056"
     CDS             52612..53289
                     /locus_tag="ACHK49_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9260762.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(53261..53974)
                     /locus_tag="ACHK49_000057"
     CDS             complement(53261..53974)
                     /locus_tag="ACHK49_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO9260763.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(53971..54480)
                     /gene="fumE"
                     /locus_tag="ACHK49_000058"
     CDS             complement(53971..54480)
                     /gene="fumE"
                     /locus_tag="ACHK49_000058"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO9260764.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(54502..55467)
                     /gene="glpX"
                     /locus_tag="ACHK49_000059"
     CDS             complement(54502..55467)
                     /gene="glpX"
                     /locus_tag="ACHK49_000059"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO9260765.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(55464..56741)
                     /gene="yggP"
                     /locus_tag="ACHK49_000060"
     CDS             complement(55464..56741)
                     /gene="yggP"
                     /locus_tag="ACHK49_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO9260766.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(56756..58144)
                     /gene="cmtA"
                     /locus_tag="ACHK49_000061"
     CDS             complement(56756..58144)
                     /gene="cmtA"
                     /locus_tag="ACHK49_000061"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO9260767.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(58172..58615)
                     /gene="cmtB"
                     /locus_tag="ACHK49_000062"
     CDS             complement(58172..58615)
                     /gene="cmtB"
                     /locus_tag="ACHK49_000062"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO9260768.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            complement(58929..60920)
                     /gene="tkt"
                     /locus_tag="ACHK49_000063"
     CDS             complement(58929..60920)
                     /gene="tkt"
                     /locus_tag="ACHK49_000063"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO9260769.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            61198..61956
                     /gene="loiP"
                     /locus_tag="ACHK49_000064"
                     /gene_synonym="yggG"
     CDS             61198..61956
                     /gene="loiP"
                     /locus_tag="ACHK49_000064"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO9260770.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(62012..62755)
                     /locus_tag="ACHK49_000065"
     CDS             complement(62012..62755)
                     /locus_tag="ACHK49_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO9260771.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(62742..63851)
                     /gene="dgaE"
                     /locus_tag="ACHK49_000066"
     CDS             complement(62742..63851)
                     /gene="dgaE"
                     /locus_tag="ACHK49_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HFO9260772.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGIAIAVAAAITRGDKARVALMPDSSGM
                     ANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGSSNLAALWQLESAITDATAALLYVK
                     SHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEEDLRAWVVSGADMVVYSGAKAFNAP
                     TSGFITGKKIWIAACKAQHHGIARAMKIGKENMVGLVYALENYHQGQAVITAEQLQPV
                     VEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQELGVDARVVEAQLRGGDIAIYARRY
                     NLHQGVFSLDPRTVAEGEMALIVARLKEIADHAKD"
     gene            complement(63855..64715)
                     /locus_tag="ACHK49_000067"
     CDS             complement(63855..64715)
                     /locus_tag="ACHK49_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO9260773.1"
                     /translation="MMSDDVMQHELVERARESGALTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEAINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(64712..65461)
                     /locus_tag="ACHK49_000068"
     CDS             complement(64712..65461)
                     /locus_tag="ACHK49_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFO9260774.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPIAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(65487..65972)
                     /locus_tag="ACHK49_000069"
     CDS             complement(65487..65972)
                     /locus_tag="ACHK49_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO9260775.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(65983..66411)
                     /locus_tag="ACHK49_000070"
     CDS             complement(65983..66411)
                     /locus_tag="ACHK49_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO9260776.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(66530..69328)
                     /gene="dagR"
                     /locus_tag="ACHK49_000071"
     CDS             complement(66530..69328)
                     /gene="dagR"
                     /locus_tag="ACHK49_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HFO9260777.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
     gene            complement(69587..70507)
                     /gene="speB"
                     /locus_tag="ACHK49_000072"
     CDS             complement(69587..70507)
                     /gene="speB"
                     /locus_tag="ACHK49_000072"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO9260778.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(70643..71374)
                     /locus_tag="ACHK49_000073"
     CDS             complement(70643..71374)
                     /locus_tag="ACHK49_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9260779.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(71520..73496)
                     /gene="speA"
                     /locus_tag="ACHK49_000074"
     CDS             complement(71520..73496)
                     /gene="speA"
                     /locus_tag="ACHK49_000074"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO9260780.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(73505..73636)
                     /gene="yqgB"
                     /locus_tag="ACHK49_000075"
     CDS             complement(73505..73636)
                     /gene="yqgB"
                     /locus_tag="ACHK49_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO9260781.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(73730..73873)
                     /gene="yqgG"
                     /locus_tag="ACHK49_000076"
     CDS             complement(73730..73873)
                     /gene="yqgG"
                     /locus_tag="ACHK49_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO9260782.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(73984..74235)
                     /gene="yqgD"
                     /locus_tag="ACHK49_000077"
     CDS             complement(73984..74235)
                     /gene="yqgD"
                     /locus_tag="ACHK49_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO9260783.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            74291..75445
                     /gene="metK"
                     /locus_tag="ACHK49_000078"
     CDS             74291..75445
                     /gene="metK"
                     /locus_tag="ACHK49_000078"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO9260784.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            75881..77275
                     /gene="galP"
                     /locus_tag="ACHK49_000079"
     CDS             75881..77275
                     /gene="galP"
                     /locus_tag="ACHK49_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO9260785.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            77352..77849
                     /gene="yggI"
                     /locus_tag="ACHK49_000080"
     CDS             77352..77849
                     /gene="yggI"
                     /locus_tag="ACHK49_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO9260786.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            77944..78651
                     /gene="endA"
                     /locus_tag="ACHK49_000081"
     CDS             77944..78651
                     /gene="endA"
                     /locus_tag="ACHK49_000081"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO9260787.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            78731..79462
                     /gene="rsmE"
                     /locus_tag="ACHK49_000082"
     CDS             78731..79462
                     /gene="rsmE"
                     /locus_tag="ACHK49_000082"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO9260788.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            79475..80425
                     /gene="gshB"
                     /locus_tag="ACHK49_000083"
     CDS             79475..80425
                     /gene="gshB"
                     /locus_tag="ACHK49_000083"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO9260789.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            80534..81097
                     /gene="yqgE"
                     /locus_tag="ACHK49_000084"
     CDS             80534..81097
                     /gene="yqgE"
                     /locus_tag="ACHK49_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO9260790.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            81097..81513
                     /gene="ruvX"
                     /locus_tag="ACHK49_000085"
     CDS             81097..81513
                     /gene="ruvX"
                     /locus_tag="ACHK49_000085"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO9260791.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(81689..82669)
                     /gene="yggR"
                     /locus_tag="ACHK49_000086"
     CDS             complement(81689..82669)
                     /gene="yggR"
                     /locus_tag="ACHK49_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO9260792.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWSARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLYQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            82687..83391
                     /gene="yggS"
                     /locus_tag="ACHK49_000087"
     CDS             82687..83391
                     /gene="yggS"
                     /locus_tag="ACHK49_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO9260793.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            83409..83975
                     /gene="yggT"
                     /locus_tag="ACHK49_000088"
     CDS             83409..83975
                     /gene="yggT"
                     /locus_tag="ACHK49_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO9260794.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            83972..84262
                     /gene="yggU"
                     /locus_tag="ACHK49_000089"
     CDS             83972..84262
                     /gene="yggU"
                     /locus_tag="ACHK49_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO9260795.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            84270..84863
                     /gene="rdgB"
                     /locus_tag="ACHK49_000090"
     CDS             84270..84863
                     /gene="rdgB"
                     /locus_tag="ACHK49_000090"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO9260796.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTHEEKSAISHRGQALKLLLDALRNG"
     gene            84856..>85969
                     /gene="hemW"
                     /locus_tag="ACHK49_000091"
     CDS             84856..>85969
                     /gene="hemW"
                     /locus_tag="ACHK49_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO9260797.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEDKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKSSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSL"
BASE COUNT        20615 a        23093 c        21592 g        20669 t
ORIGIN      
        1 taaatggccg cttcggcggc ctgtttctct gtttgaacga tgactaaata gtgactgcga
       61 aaatgagata agccgttctc aaggcgtcct gcgccccaaa ttcgtgggaa tttattttac
      121 taattcagat gatcaaattt actttaaaag gagtgaaggg atgtctgata taaaccatgc
      181 aggttctgac cttatatttg aactggagga tcgccctccc tttcatcagg ctctcgttgg
      241 tgccattacc catctgttgg caattttcgt tccgatggta acccccgcgt taatcgtggg
      301 tgcggcctta cagctttccg ctgaaacaac cgcctatctt gtttctatgg cgatgatcgc
      361 ctctggtatt ggtacctggt tacaagtaaa ccgctacggc atcgtcggtt ctggcctact
      421 ctcaattcag tcagtcaatt tttcatttgt tacggtcatg attgcgctgg gcagcagcat
      481 gaaaagcgac ggttttcacg aagagttaat catgtcgtcg cttctcggcg tctccttcgt
      541 tggcgcattt ctggttgtcg gatcttcatt tatcttgccc tatttacgtc gggttattac
      601 gcctaccgtc agcggtattg tggtactgat gatcggctta agcctgatta aagtcggcat
      661 tatcgatttt ggtggaggat ttgcagccaa aagcagcggt acgttcggca attacgaaca
      721 tctcggcgtt ggtttattgg ttttaattgt ggtgatcggc tttaactgct gtcgcagtcc
      781 gttgctacgc atgggaggga tcgccattgg gctatgtgtc ggctatatcg catcgttatg
      841 cctgggcatg gtggatttca gcagtatgcg caatttgccg ttaatcacca tcccgcatcc
      901 gttcaaatac ggctttagtt ttagcttcca tcagttcctg gtggttggca cgatttatct
      961 gcttagcgtg ctggaagcag tcggcgatat caccgccacg gcaatggttt cccgccgccc
     1021 cattcagggg gaagagtatc agtcccgact gaaaggcggc gtgctggcag atggtctggt
     1081 ttctgttatc gcctccgctg tcgggtcatt acccttaacc acgtttgcgc aaaataatgg
     1141 ggttattcag atgactggcg tcgcttcacg ttatgtcggg cgaaccatcg cggtaatgct
     1201 ggttatcctc ggtttgtttc cgatgattgg cggcttcttc acgaccattc cctcggcagt
     1261 tctgggaggc gcaatgacgt tgatgttttc catgattgcc atcgctggga ttcgcataat
     1321 catcaccaac ggtttaaagc gccgagaaac acttattgtc gccacttctt taggtttagg
     1381 acttggcgtc tcctacgatc ccgaaatttt taaaatattg ccagcctcta tttatgtact
     1441 agttgaaaac cctatttgtg ctggcgggtt aactgcgatt ttattaaata ttatcctccc
     1501 tggtggctac cgacaggaaa acgttctgcc tggtattacc tcagcggaag agatggatta
     1561 acagtaaagg agtcaatgat gtcaggagaa cacacgttga aagcggtacg aggcagtttt
     1621 attgatgtca cccgtacggt cgataacccg gaagagattg cctctgcgct gcggtttatt
     1681 gaggatggtt tattactcat taaacaggga aaagtggaat ggtttggcga atgggaagac
     1741 ggaaagcatc aaattcctga cactattcgc gtgcgcgact atcgcggcaa actgatagta
     1801 ccgggctttg tcgatacaca tatccattat ccgcaaagtg aaatggtggg ggcctatggt
     1861 gaacaattgc tggagtggtt gaataaacac accttcccta ctgaacgtcg ttatgaggat
     1921 ttagagtacg cccgcgaaat gtcggcattc ttcatcaagc agcttttacg taacggaacc
     1981 accacggcgc tggtgtttgg cactgttcat ccgcaatctg ttgatgcgct gtttgaagcc
     2041 gccagtcata tcaatatgcg tatgattgcc ggtaaggtga tgatggaccg caacgcaccg
     2101 gattatctgc tcgacactgc cgaaagcagc tatcaccaaa gcaaagaact gattgaacgc
     2161 tggcacaaaa atggtcgtct gctatatgcg attacgccac gcttcgcccc gacctcatct
     2221 cctgaacaga tggcgatggc gcaacgcctg aaagaagaat atccggatac gtgggtacat
     2281 acccatctct gtgaaaacaa agatgaaatt gcctgggtga aatcgcttta tcctgaccat
     2341 gatggttatc tggatgttta ccatcagtac ggcctgaccg gtaaaaactg tgtctttgct
     2401 cactgcgtcc atctcgaaga aaaagagtgg gctcgtctca gcgaaaccaa atccagcatt
     2461 gctttctgtc cgacctccaa cctttacctc ggcagcggct tattcaactt gaaaaaagca
     2521 tggcagaaga aagttaaagt gggcatggga acggatatcg gtgccggaac cactttcaac
     2581 atgctgcaaa cgctgaacga agcctacaaa gtattgcaat tacaaggcta tcgcctctcg
     2641 gcatatgaag cgttttacct ggccacgctc ggcggagcga aatctctggg ccttgacgat
     2701 ttgattggca actttttacc tggcaaagag gctgatttcg tggtgatgga acccaccgcc
     2761 actccgctac agcagctgcg ctatgacaac tctgtttctt tagtcgacaa attgttcgtg
     2821 atgatgacgt tgggcgatga ccgttcgatc taccgcacct acgttgatgg tcgtctggtg
     2881 tacgaacgca actaataata aaactttaac atcctcgtga ggacatcatt atgtctggag
     2941 acatcctaca aacaccggac gcaccaaagc cacagggcgc gctggataat tattttaaaa
     3001 ttaccgctcg tggcagtacc gttcgtcagg aagtactggc tggcttaacg acctttctgg
     3061 ccatggttta ttccgttatc gtcgttccgg gaatgctggg caaagcaggt tttcctcccg
     3121 cagctgtgtt tgttgccacc tgtctggtcg cgggcttcgg ctcgttgctg atggggttgt
     3181 gggccaattt gccaatggcg attggttgtg cgatttcctt gacggcattt accgcattca
     3241 gtctggtact cgggcaacaa attagcgttc ctgtcgcact gggcgcggtc tttctgatgg
     3301 gcgtcatctt caccgctatt tccgtaaccg gtgtgcgtac ctggatctta cgtaatttac
     3361 cgatgggtat cgctcacggt acaggtatcg gtatcggcct gtttctgctg ctgattgctg
     3421 ctaacggtgt gggtatggtt atcaaaaacc cgattgaagg cttgccagtg gcgctcggtg
     3481 cgtttacctc cttcccggtg atgatgagct tgctggggct ggcggtcatc ttcggtctgg
     3541 agaagtgccg cgtacccggc gggatcttgt tggtgattat cgcaatttcg attatcggct
     3601 taatctttga cccagcggtg aaataccacg gtctggtggc gatgccaagt ctgactggcg
     3661 aagatggtaa gtctctgatt ttcagcctcg atattatggg cgcactccag ccaaccgtac
     3721 ttccgagtgt actggcattg gtgatgaccg cagtgttcga cgccactggc accatccgtg
     3781 ccgtcgccgg tcaggcgaat ttgttggata aagacaacca gatcatcaat ggcggcaaag
     3841 ccctgaccag tgactcagta agttcaatat tctccggtct ggtgggcgca gcgcccgcag
     3901 cggtttatat cgaatcagcg gcaggaaccg ccgccggggg taaaacaggg ttgaccgcaa
     3961 ccgtagtggg ggcgttattc ctgctgattc tgttcttatc accgctgtca tttttgatcc
     4021 ctggttacgc cactgcaccc gctctgatgt acgtgggttt gctgatgtta agtaacgtct
     4081 cgaagctgga tttcaacgat tttattgacg caatggctgg cctggtgtgt gccgtgttca
     4141 tcgttctgac ttgtaatatc gttaccggta ttatgctggg ctttgtgaca ctggtcgtag
     4201 gccgtgtctt tgctcgcgaa tggcaaaagt tgaatattgg tacggtgatc attactgccg
     4261 cactggtcgc attttacgcg ggtggttggg caatctaata gattctccgc gcctttcagc
     4321 gcggagattt ctttcacaga ggattctcac cgctggcaac aatacgctgc tggcggactc
     4381 gcctgagttc tctttcagta agcaactgca gcgcctgtgt cgggcaagat tcaacacagg
     4441 ctggcccctc ttcccgctgt tcacagagat cgcatttcac aatttgttgt cgggtatccc
     4501 ccggcaacga ctgaatggtg atcattccaa acgggcacgc actgacgcaa ctctgacagc
     4561 caatacaacg ggcagaattg gtctgcacca cctgctcgcc catcgtcagc gcccccacgg
     4621 ggcaagcgcc aacacaaggg gcgttttcac actgatggca catcaccggc gcactgatgc
     4681 tgtccagtcg ctgtaccttc agccggggta aaaagacatc ggcattcaac tcctgttctg
     4741 aagggtgagc gaccacacag gctacttcac aagtacggca gccaatacag tcagccggat
     4801 taacgataat taacgatttc atcacgatgc cttcgtatca aacagagtta acatatcgcg
     4861 cgccgcctgc cttcctgcgg ccattgcagt gacaaccaga tccgcgccat gaactgcatc
     4921 accaccagca aagacttttt tcagatgcgt ctgggtaggt aaatacccga cgtcaccggt
     4981 ttgaatcagg ccccatttat cgagtttaat tccgctgccc tgcaaccacg gcatggcatg
     5041 cgcctggaaa ccaaaggcca taatcagaac atcggcgggc aattcaaact ctgaacccgc
     5101 taccggacga ggacgacggc gaccatccgg ccccggctca cccatggcgg tacgaatcag
     5161 gcccaccgca gttaaacgcc catcttcgtc acaagcgata tattgcggtt gaacattgaa
     5221 ctgaaactcg acaccttcct cgcgcgcatt aaccacctct ttgcgcgagc ccggcatact
     5281 gacttcatca cgacgatacg cgcaggtcac gctggcggca ttgaggcgga tggaagtccg
     5341 caaacaatcc attgtcgtat cgccaccgcc caataccacg actcgcttac cttccacgtc
     5401 cgtcagcgga tactcttcag actccggcaa tcccatgagc tggcgggtat gggcagtcag
     5461 gaacggtaga gcctgaatga caccgggcgc atcttcatgc ggcagatctg ctcgcatcat
     5521 cccgtaagtc cccacgccga tgaaaactgc atcatattca gaagttaaat cgctaaaggt
     5581 aatatcgcgg ccaatttcac agttaagatg aaagtcgatt cccattgcgg tgaatatctc
     5641 tcgccgctgg cttaataccg ttttatcgag tttgaaagga ggaatgccaa aagtcagcat
     5701 accgccaatt tctggatggc gatcaaagac atcaacctga actcccgcgc gcgccagaat
     5761 atcagcacac cctaaccctg ctggcccagc gccaatcacc gccacttttt cgctacgggg
     5821 aacaactttg ctgacatcgg gacgccagcc catcgccagc gcggtatcgg tgatgtagcg
     5881 ttccagatta ccgatagaga ctgcgcccga gtgatctttc aaagtacatg cgccttcaca
     5941 aagacggtcc tgtggacata ccctgccgca aatttctggt aaggaactgg tctggtggca
     6001 aagttctgcc gcttcaataa tctttccttc ctgtaccaga cggatgtaat ccggaatagc
     6061 gttatgcagc gggcaatgcc agttgcagtt agctttttcg gcacaataaa cacagcggtc
     6121 actctcataa gtcgcttgca gtggatccag cccgcaatat atttcgccaa agtgggtttt
     6181 ccgttcactc gctgaaattt tatctgcgcc tttacgcgag ttaacgggga gcaacgctgc
     6241 actgcgagat ggctgagcat ctgatgacgc ttttcctgct gccgttttgc gctggcgagc
     6301 cacctttatc cactgtaacc ctttatcgtc catcagtcgt aacgcctgtg ttgggcagac
     6361 ttcaatacag gcttgcgtgc cggaactgcg ctggttacaa aggtcgcatt tctgcgcaat
     6421 cgtatcgacc atctcaacga cgccaaaggg gcaagcgatt gcgcatcttt tacagccaat
     6481 acatttttgc tcgtccagtt gtacgctatc ggactggaaa gtcagagcat taaccggaca
     6541 agccgtaacg caaggggcat tgttgcagtg atggcaggcc accggattcg cagcctggcc
     6601 tttccctaca acgtggatac gcggtcgaaa gtcactgtga ctcagcggcc agttttcttg
     6661 attgtgtgcc accgcacagg caatttcaca agcatgacag cctatacatt ccgcagcttc
     6721 agcagcgata aacttattca tttgcatccc tttcatttaa tgagttatgt cttctcaaat
     6781 ttcgcgatgc aataaacggg catatattag gtagcatgac cgttttaatt ataattttgc
     6841 gactgagttc aaattattgc ccctcaatct atgccgcttt cattatttca acattgccga
     6901 ccaaaagagc gctatccaga taaaaacgat taaatgcgag agtgcgacat gccagaatga
     6961 ttaatgaaat atcaatatga gaaaattaca gataatttca ttgtcaggtt ctcttctcat
     7021 tttcgtggtt taaccacgat tatgtgattg cctccgcaat tcctgtctct aactcccctt
     7081 cctcgcaaaa actggcactc cacgagcatg tgtttagaca gtttcattaa cgtaaacggt
     7141 tgctttttac tctggcgggc gaaaggagaa acactgatga gcgccataga ttcccaactt
     7201 ccctcatctt ctgggcaaga ccgcccaact gatgaggttg accgcatatt atcaccagga
     7261 aagctgatca tactcggtct gcaacacgtc cttgtcatgt acgcaggtgc agtcgctgtt
     7321 cctcttatga ttggtgaccg actgggcctc tcaaaagaag ctattgcgat gctcattagc
     7381 tcggatctct tttgctgcgg gatcgtcaca ttattgcaat gtatcggtat cggccgcttt
     7441 atggggatcc gcctgccggt gattatgtcg gtgacctttg ctgctgtaac accaatgata
     7501 gccattggga tgaacccgga tatcggcctg ctggggatat ttggtgccac tatagccgcg
     7561 ggttttatca ccacattatt agcgccactt attggtcgct tgatgccttt attcccgcca
     7621 ctggttaccg gtgtggttat tacttctatt gggcttagca tcattcaggt gggtattgac
     7681 tgggccgccg gaggtaaagg gaatccgcaa tatggtaatc ccgtttattt aggtatctcc
     7741 tttgccgtct taatttttat cttgctcatt actcgctatg cgaaaggatt tatgtccaac
     7801 gtcgccgtat tactggggat tgtatttggc tttttacttt cgtggatgat gaatgaagtc
     7861 aatttatccg ggctacatga tgcctcgtgg tttgcgattg tcaccccgat gtcgtttggt
     7921 atgccgattt tcgatcccgt ttccattctg accatgactg ccgtgttaat catcgtgttt
     7981 atcgagtcga tggggatgtt cctggcactg ggtgaaatag tcggtcgtaa actctcttca
     8041 cacgatatta ttcgcgggct gcgtgtcgat ggcgtaggga caatgatagg cggcacgttt
     8101 aacagcttcc cccacacgtc attttctcaa aacgttggcc tggttagcgt gacgcgcgtt
     8161 catagccgct gggtgtgtat ttcttcggga attatattaa tcctgtttgg catggtgcca
     8221 aaaatggcgg tgctggtagc ctccattccg caatttgtgc tgggcggcgc tgggctggtg
     8281 atgttcggca tggtactggc gacagggatt cgaattctgt cgcgctgtaa ctacaccacc
     8341 aaccgttaca acctctatat tgtggcgatc agtctcggcg ttggcatgac tccgacgctc
     8401 tctcacgatt tcttttctaa gttaccggcc gtactgcaac cgttgctgca tagcggcatt
     8461 atgctcgcaa cccttagcgc cgttgtgctg aatgtcttct ttaatggcta tcagcatcat
     8521 gctgacctgg tgaaggaatc cgtctctgat aaagatttaa aagtcaggac agtacgtatg
     8581 tggcttctga tgcgcaagct gaagaaaaat gagcatggag aataatatga attttttaat
     8641 gcgcgctata ttcagtctgt tgttgctttt tactctctct attcctgtca tttctgactg
     8701 tgttgcaatg gccattgaaa gtcgcttcaa atatatgatg ctactttttt aaatggtttt
     8761 tacctgtcgg catccgctca aaacgggcgg ttgtcgataa acgctcactt ggttaatcat
     8821 ttcactcttc aattatctat aatgatgagt gatcagaatt acatgtgaga aattatgcaa
     8881 acggaacacg tcattttatt gaatgcacag ggagttccca cgggtacgct ggaaaagtat
     8941 gccgcacaca cggcagacac ccgcttacat cgcgcgttct ccagttggct gtttaatgcc
     9001 aaaggacaat tattagttac ccgccgcgca ctgagcaaaa aagcgtggcc tggcgtgtgg
     9061 actaactcgg tttgtgggca cccacaactg ggagaaagca acgaagacgc ggtgatccgc
     9121 cgttgccgtt atgggcttgg cgtggaaatt acgcctcctg aatctatcta tcctgacttt
     9181 cgctatcgcg ccaccgatcc gaatggcatt gtggaaaatg aagtgtgtcc ggtatttgcc
     9241 gcacgcacca ccagtgcgtt acagatcaac gatgatgaag tgatggatta tcaatggtgt
     9301 gatttagcag atgtattaca cggtattgat gccacaccgt gggcgttcag tccgtggatg
     9361 gtgatgcagg cagccaatag tgaagcaaga aaattgttgt ctgctttcgc gcagcacaat
     9421 taaaaaaccc cgacatttgc cggggttgtg agcataacgt aatgcttatt ttaccggacg
     9481 catcgccggg aacagaataa cgtcgcggat ggtatggctg ttggtgaaca gcattaccat
     9541 acggtcgatg ccaatcccca gacctgctgt cggcggtaag ccatgttcca gtgcggtaac
     9601 gtaatcttca tcgtagaaca tcgcttcgtc gtcacctgcg tctttcgcgg caacctgatc
     9661 caggaagcgt tgcgcctgat cttccgcgtc attcagctcg ctaaagccgt taccgatttc
     9721 acgaccaccg atgaagaact caaagcggtc tgtgatttcc gggttaatgt cgttacgacg
     9781 cgccagcgga gaaacttctg ccggatattc agtaatgaag gtcggctgaa tcagatgtgc
     9841 ttctgccact tcttcgaaga tctcggtaac gatacggccc agaccccagc tcttctcaac
     9901 gtggataccg atagattcag caatcgcttt cgcagagtcg aagttatcca gatccgccat
     9961 gtcggtttcc gggcgatatt tcttgatcgc ttcacgcatg gtcagttttt cgaacggttt
    10021 accgaagtcc agcgtcacgt cgccgtaggt cacttccgtc ttaccgagaa tatcctgtgc
    10081 cagagtacgg aacagcgatt cggtcagctc gatcagatct ttgtaatctg cgtaagccat
    10141 gtagagttcc atcatggtga actctgggtt atgacgtacg gaaatacctt cgttacggaa
    10201 gttacggttg atttcgaata cacgctcgaa gccaccgacc accagacgct tgaggtacag
    10261 ttccggcgcg atacgcaggt acatgtcgag atccagcgcg ttatggtggg tgataaacgg
    10321 acgcgcagcg gcaccgcctg ggatcacctg catcatcggc gtttcaactt ccataaagcc
    10381 gcggttcacc atgaactggc gaataccaga gaggatctgc gagcgtactt taaaggtgtt
    10441 acgggattca tcgttagaga tgagatccaa ataacgctga cgatagcgcg cttcctgatc
    10501 ctgcaagccg tggaatttat ccggcagcgg acgcagtgct ttggtcagca gacgcagctc
    10561 ggtgcagtgg atagacagtt cgccggtttt ggttttgaac agtttacctt tcgcgccgag
    10621 gatgtcgccg aggtcccatt ttttgaactg ctcgttgtaa acgccttccg gcagatcgtc
    10681 acgggcaacg tacagctgaa tgcggccacc cacatcctgc agggtaacga aagacgcttt
    10741 acccataata cggcgggtca tcatgcggcc agcaacggcg acttcgatgt tcagcgcttc
    10801 cagttcttcg ttctctttac cgtcgaattc tgcgtgcaat tggtcagagg tatgatcgcg
    10861 acggaaatcg ttcgggaagg caaccccctg ctcgcgcagg ttcgccagct tctcacgacg
    10921 cgttttcagt tcattgttaa gatcgactac cgcgtcagcg ccctgtgcgt gttgttcaga
    10981 catgttggtt cctcataacc ctgctttcaa acttgcttcg ataaattgat ccaggctgcc
    11041 gtccagcacg gcctgcgtgt tgcgggtttc taccccggta cgcagatctt taatgcggga
    11101 gtcatcaagg acataagaac gaatctggct gccccagccg atgtcggatt tgttatcttc
    11161 catcgcctgt ttctcggcat ttttcttctg catctccagt tcataaagct tcgctttcat
    11221 ctgcttcatg gcctggtctt tgttcttgtg ctgggaacgg tcgttctggc attgggtcac
    11281 gatcccggtt gggatgtggg taatacgcac cgcagattcg gtacggttaa cgtgctgacc
    11341 gcccgcgccg gacgcgcgat aaacgtcaat gcgcagatcc gccgggttga tttcgatatc
    11401 aatatcatca tcaacttccg ggtaaacaaa cgcagagctg aacgatgtgt ggcgacgacc
    11461 gccggagtca aacgggcttt tacgcaccag gcggtgaacg ccagtttctg tacgcagcca
    11521 gccgtaagcg taatcgccgg agattttgat cgtcacggat ttaatacccg ccacttcacc
    11581 ttccgactct tcgatgattt cagttttgaa accacgcgat tcagcccagc gcagatacat
    11641 acgctcaagc atgctcgccc agtcctgtgc ttccgtaccg ccagaccccg cctgaatatc
    11701 gaggtagcag tcggcgctgt catattcgcc agagaacata cggcggaact caagctgcgc
    11761 cagtttttct tccagggcgt cgagttcagc aacggcttcg ttaaaggttt cttcgtcgtc
    11821 agcttctaca gccagttcca gcagaccaga aacatcttcc agcccctgtt tcatttggtc
    11881 gagggtgtcg acaacggctt cgagggagga acgctcttta cccagcgcct gtgcgcgttc
    11941 gggttcgttc cagacatccg gctgttccag ctcggcgttt acttcttcca gacgctcttt
    12001 cttggcgtcg tagtcaaaga taccccctaa gaacgtcgga gcgttccgtg aggtcctgaa
    12061 tgcgattatt taccggatta atttcaaaca tggtctgatt tcttttattg agctagtcaa
    12121 aatgcggtga taagagcggg attgtaccca atccacgctc ttttttatag agaagatgac
    12181 gctaaattgg ccagatattg tcgatgataa tttgcaggct gcggttgccg cgaaactcgt
    12241 tgatatcgag cttataagcc agttgcactt cacgcacgcc gttatccggc cagagggcgg
    12301 tatcgacatt aaaagcaata ccatccagca gtggaccgcc gccgaccggt tcgaccatca
    12361 ccttcaaatg acgttcgccc accagccgct gttgcagcag acggaaatga ccgtcaaaca
    12421 gcggctccgg gaacatctgc ccccacgggc cagcatcgcg cagcaactgc gccacttcca
    12481 tggtcatttc ggccgggctt aacggaccgt ctgacaccac ttcgccttgc aatagcgaag
    12541 ggtccagcca ctctgtaacc agttcgccaa accgttgttg aaagagttcg aatttatcct
    12601 cttccagcga caaacccgcc gccatcgcat gaccgccaaa cttcagtatc atgccagggt
    12661 agagtgtgtc taatcgctcc agtgcatcac gcatatgcag cccctgaatg gagcgacctg
    12721 aacctttcag cgtaccatca cctgctggcg caaaggcgat aaccggacgg tgaaaacgct
    12781 ctttgatgcg cgaagccaga ataccgacaa cgccctgatg ccattcgggg tgatacattg
    12841 ccagcccgcc gggtagcgtg tcgcgacttc gctccagttt ctcgcacagg gtcagggctt
    12901 caacttgcat tccttgttcg atctcttttc gcgtctggtt tagcgcatcg agttcatttg
    12961 ccagcacgcg cgcttcgccg atgttgtcgc acagcaacag cgccacaccg acggacatat
    13021 cgtccagtcg cccggcagca ttgagacgtg gccccagcgc aaaacctaaa tcgctggcgg
    13081 cgagtttttg cgcatcgcga tttgccactt ccagcagcgc tttaatcccc ggacggcact
    13141 ttccggcacg gatgcgactc atcccctgcc aggtcagaat gcgattatta gcgtccagcg
    13201 gcacgacgtc cgccactgtt cccagcgcga ccagatccag cagttctgcc aggttaggaa
    13261 ttgcgatgcc acgctcatca aaccagccct gatcgcgcaa aaaggtgcgc agcgccagca
    13321 tcagataaaa cgccacaccc acgcctgcca gtgatttcga cgggaaatta cagtcgcgca
    13381 agttagggtt aatgatcgct tccgccgcgg gtaatgtttc gcctggcaaa tggtgatcgg
    13441 taacaataac cggaatgccc aacgagcgag cgtgtacaac ccccgcatgg gaggaaatac
    13501 cgttatccac cgtgacaatt aactgcgcgc cacgggcatg ggcctgatcg accacttccg
    13561 ggcttaagcc gtaaccgtct tcgaaacggt ttggtaccag gtagtcgata ttgctgcaac
    13621 caagcgagcg catcgccagc acgcttagag ccgtgctggt cgcgccgtcg gcgtcgaaat
    13681 caccgaccac aataatccgc gttccttcgc gaaaagcgtt gtaaaggatc tcaacggcct
    13741 tttcgacgcc gctcagttgc tgccagggca acataccttt aacactgcgt tccagttctt
    13801 gcgcactgcg cacgccccgg ctggcgtata aacggcgcag caagggaggc aattcagcgg
    13861 gcaagtctgc cgtttcatcg acttcacggc gacgaagttg tatctgttgt ttcacgcgaa
    13921 ttatttaccg ctggtcattt tttggtgttc gtcgaggaat tctttcatct ctttcggcgg
    13981 ctggtaaccc ggaacaagtg tgccattgct cagcacaact gccggagtac cgctaacgcc
    14041 aagctggacg ccaagtgcgt aatggtcggc aatatccacg tcgcagctgg ctggtgcgac
    14101 gcttttacct gccatcacat catcaaacgc tttgttttta tctttcgcac accagatagc
    14161 tttcatttct ttctctgcat cgctgtccag cccctggcgc gggaaagcaa gataacgcac
    14221 ggtgatcccc agcgcgttat agtctgccat ttgctcatgc agtttgtggc agtaaccaca
    14281 ggtaatatca gtaaacacgg tgatgacgtg tttttcctgc ggcgctttat aaacgatcat
    14341 ctctttttca agcgcattca actgctttaa cagcatctta ttggtgacat tgaccggagc
    14401 cgtgccgcta acgtcataca ttggcccctg aatgatatgt ttaccatcat cggtgatgta
    14461 caacacgccg ctgttagtca gaactgtctt catgccagct acgggcgcgg gctgaatatc
    14521 gctgcttttg atgcccattt tggctaacgt ttgttgaatt gccgcgtcat cagcctgagc
    14581 aaaacctgaa aacgccgcta acaaagtaaa caacataaaa cctttcttca taaatcttcc
    14641 cgttcttttc agacatcacg cccgcgggtg atgctgttga tgaagttgcc gcagacgctc
    14701 ggtagcgaca tgcgtataaa tttgcgtggt ggaaagatcg ctgtggccca gtagcatctg
    14761 caccacgcgt aaatccgcac catgatttaa taaatgagtg gcaaaagcgt gacgcaacac
    14821 atgcggtgac agcttttcgc tgtcgatacc cgccagcaca gcataatgtt taatgcggtg
    14881 ccagaaggtc tgtcgcgtca tctgctgcgc acgctggctg ggaaacaaca cgtcaattga
    14941 cacaccattc agcagccacg gacgcccatg ttccagatag gtttccagcc agtaaaccgc
    15001 ctcttcacct aacggtacca ggcgctcttt gttgccttta ccaatgaccc gcactacgcc
    15061 ctgacgcagg ctgatatcac tcattgtcag tccgaccagt tcagagacac gcagcccggt
    15121 agcatacaac acttcaagca tggctttatc gcgtagctcc agtggctgat caattaatgg
    15181 tgcccgtaat aaacgttcga cctgcgcttc gcttaaatct tttggcaaac gctggggcaa
    15241 tttcggtgaa gcgagatgcg cactgggatc gtcttcacga aacttttcgc gataaagata
    15301 ctggaacaat cggcgcactg cactcagcaa acgcgctgag ctggtggctt tatacccgcc
    15361 ctccagccgt tctgccagta atgcctgcaa atcgtcactt tgcgccgtcg ccagcgtcaa
    15421 cccgcggtga tgcaaccact ccaccatcat tgacagatcg cgacggtaag cgttcaacgt
    15481 attttcagcc agattttttt ccagccacag agcatcaaga aactgctcga tgcgtgccag
    15541 atcctgtttc acttgcgccc cttatggtca ctcatttgat ccattatgcc ttattgtgcc
    15601 gtgactaaag cgattctgat acactagctg caaaagccac agcagaatcg agaagcttac
    15661 gttatgaata tgggtctttt ttacggttcc agcacctgtt acaccgaaat ggcggcagaa
    15721 aaaatccgcg atattatcgg cccagaactg gtgaccttac ataacctcaa ggacgactcc
    15781 ccgaaattaa tggagcagta cgatgtgctc attctgggta tcccgacctg ggattttggt
    15841 gaaatccagg aagactggga agccgtctgg gatcagctcg acgacctgaa ccttgaaggt
    15901 aaaattgttg cgctgtatgg gcttggcgat caactgggat acggcgagtg gttcctcgat
    15961 gcgctcggta tgctgcatga caaactctcg accaaaggcg tgaagttcgt cggctactgg
    16021 ccaacggaag gatatgaatt taccagcccg aaaccggtga ttgctgacgg gcaactgttc
    16081 gtgggtctgg cgctggatga aactaaccag tatgacctta gcgacgagcg tattcagagc
    16141 tggtgcgagc aaatcctcaa cgaaatggca gagcattacg cctgatctca ctgacggctt
    16201 agcgcatatg ctttgccgtc atctttatct ttgcgtctct tgttgcaaca aaatccgccg
    16261 taaatcccgc cattcagctt cgtccatgct gtcggctgcc agccataaat gttgccgttt
    16321 accgccatca gaacgtaaac gcagcatcat gccgctctta atcatccacg gtgctttgac
    16381 gatgctccac tcctgccctt gccaacgcaa acgcccgtcc atcaacaagc gaatttcccc
    16441 ctggcgagca ttaatacgcc gctggctgcg aacgcaatca aacaccacca gcgaaagtaa
    16501 caccatccat aacggggtgt aactgagtgg ccagggcatg agtaaaataa cagcggcaac
    16561 cagcccatga atcagcaagg aaagccactg tgcgcgccag gagacgcgca aatcagattg
    16621 ccacaggacc acgttcccgg ttccgtgtct ggatgagtcg gaccatcatt tccagttctg
    16681 catcggccgg tttaccgtga ttcatcagcc agttaaacag gtccggatcg tcacattcca
    16741 gcagacgaat aaagatgcgt ttttcgtcat cgcttaagct gtcgtactca tgttcgaaaa
    16801 acggcatgat tgaaatatcg agttcgcgca taccacggcg gcatgcccaa tgaatgcggg
    16861 ctttgttgtt aatgtccatc ttcttcctgt ctcacgaaaa tccagtaccc cgctattgta
    16921 acgtgttttt cgccttcttt tacgggaata tcagtaaaca ccatcgcgat cgcgaaatta
    16981 attcacaaca attcaatggc ttcatttttt tggaagtcgc ctcgcagaag gcacagatcg
    17041 cgtagtgaaa gcacttgcat tgcctcatag ctcttttacc attagtcatt aatacgccgt
    17101 taagcaactc aggactctat tatggctttt acaccttttc ctccccgtca gcctacggct
    17161 tctgcccgtt taccgctgac gctgatgacg cttgatgact gggcgcttgc caccattact
    17221 ggcgcggaca gcgaaaaata tatgcagggt caggtgacag cagatgtcag ccagatgaca
    17281 gaaaatcagc acctgctcgc cgcccattgc gacgccaaag gtaaaatgtg gagcaattta
    17341 cgtctgttcc gcgacggcga tggctttgca tggattgaac ggcgcagcgt gcgtgaaccg
    17401 cagctgactg aactgaaaaa atatgcggta ttctctaaag tgaccatcgc gccagacgac
    17461 gagcgtgtgc tgcttggtgt tgccggtttt caggcgcgcg ccgcgctggc aaatctcttt
    17521 agcgaactgc cttcgaaaga aaaacaggta gtcaaagaag gcgcgaccac tttgctatgg
    17581 tttgaacacc cggcagaacg tttcctgatc gtaaccgatg aagctactgc taatatgctg
    17641 accgataaac tgcgcggtga agcggaactg aacaatagcc aacagtggct ggcattaaac
    17701 attgaagcgg gtttcccggt gattgatgcc gccaacagcg ggcagtttat cccacaggcg
    17761 accaatctcc aggcgctggg cggtatcagc tttaagaaag gctgttatac cggacaagag
    17821 atggtggcgc gagcaaaatt ccgtggtgcc aataaacgtg cgctctggtt gctggcaggt
    17881 agcgccagcc gactgccgga agctggtgaa gacttagagc tgaaaatggg cgagaactgg
    17941 cgtcgtaccg gtacggtgct ggctgcggta aaactggaag atggtcaggt cgtggtacag
    18001 gtcgtcatga ataacgatat ggaaccggat agcatcttcc gcgtacgcga cgatgcgaat
    18061 acattgcata tcgagccgct gccgtattcg ctcgaagagt aaatctcttt atcgcatcag
    18121 gcatttatcg cctgatgcga cgctgacgcg tcttatcatg tctgggattt gatgccttta
    18181 tcacaacagg gacgaggccc gtagatcgga taaggcgttc acgccgaatc cggcagttgt
    18241 gctccgatgc ctgatgcgac gctggcgcgt cttatcaggc ctacaaaggc atacccatta
    18301 cgcctgccca atatacaaat agatcgccag aaagtggcac acactaccgc cgagcacgaa
    18361 gccgtgccag atggcatggt tgtatggaat gcgtttgcag acgtagaaaa tcaccccgag
    18421 cgaataaacc acgccgccta ccgccagtaa ggtaacgctg cccgccgcga gcttaactgc
    18481 catttcataa attaccacca gcgacagcca gcccatcgcc agataggtca ccagagataa
    18541 aattttgaat cggtgcgcga tggtcagttt aaacagaata cccagcaatg ccaggctcca
    18601 gataacaatc atcaacccgc gcgctaacgg agaatccagc cccaccagca aaaacggcgt
    18661 gtaggttccg gcaatcaaca ggtaaatagc gcaatggtca aatttcttca gccacatttt
    18721 tgcccgttga tgaggaatgg cgtgatagag cgtcgaagcg aggaacagca ggatcatact
    18781 gccgccatag aggctgtaac tggttatcgc agtggcgctg gcattgagat ccaccgcctg
    18841 aaccagtagc aacaccagcc caacgatacc aaacaccaac ccaatgccgt gactgacgct
    18901 gttggcaatt tcctctgcca gcgaatatcc ctgcttaatg aggggcttct gaaccataac
    18961 ttactccgga gaaacgtaca cgcacatgta tacctctcca gcgtaactga gaatagttcc
    19021 agtgaacacc tgttagctaa aataaattct gatttgagac taatctccta aaaatcatga
    19081 aattaaatgc gaaatttcaa ctaacaggcg cgagttcaat ttaaagacat ttaaattcaa
    19141 tcacataaaa ctgtgtctga ccgggataga tgtcggcaat gacctttatc agttcagtca
    19201 gggtcatatt ttcctgctct gcatgttttt cagtcagcgt atccagcgtt acggttgagg
    19261 ttgcggtgac ttcaatcgtg caaaaataac cgtcatcttc aaaacgtccg acacgaagca
    19321 catcacccgt tttgaagtgc gattcagact cgtcgcggat ggtgatggtt ttacgcccag
    19381 ccagaatgtc atcctggaaa cgttgaaaaa aagtgatgtc gtttggctgc atggtactat
    19441 ttcctgtaag aattgactca tctggagcct atgatagtga aaaaactcac cttaccgaaa
    19501 gatttcttat ggggcggcgc agttgccgct catcaggtcg aaggcggctg gaacaaaggc
    19561 ggaaaagggc cgagcatttg tgacgttctg accggtggcg cacacggcgt gccgcgcgaa
    19621 atcaccaaag aagtcttgcc aggcaaatac tatccaaacc atgaagccgt tgatttttat
    19681 ggtcactaca aggaagacat caagctattt gccgaaatgg gcttcaaatg ttttcggact
    19741 tccatcgcct ggacgcgtat ttttccaaaa ggtgatgaag ctcagccaaa cgaagaaggg
    19801 ctgaagttct acgatgatat gttcgatgaa ctgctgaaat acaacatcga accggtgatc
    19861 accctctccc actttgaaat gccgctgcat ctggtgcagc aatacggtag ctggaccaac
    19921 cgtaaagtgg ttgatttctt tgtccgtttc gcggaagtgg tgtttgaacg ctataagcat
    19981 aaagtcaaat actggatgac cttcaacgaa attaacaacc agcgtaactg gcgtgcaccg
    20041 ctgttcggtt actgctgctc cggcgtggtt tataccgagc atgagaaccc ggaagagacg
    20101 atgtatcagg tgctgcatca ccagtttgtc gccagcgccc tggcggtgaa agctgcgcgt
    20161 cgcattcacc cggagatgaa agtcggctgt atgctggcga tggtgccgct ctatccctac
    20221 tgctgtaacc cggacgatgt gatgttcgct caggagtcga tgcgcgaacg ctacgtcttt
    20281 accgatgtgc agctacgcgg ctattacccg tcctatgtgt tgaacgagtg ggagcgtcgc
    20341 ggatttaaca tcaaaatgga agacggcgat ctcgatgtac tgcgtgaagg cacctgcgat
    20401 tatcttggtt tcagctatta catgaccaac gcagtgaagg ccgaaggcgg caccggcgat
    20461 gcgatctctg gtttcgaagg cagcgtaccg aacccgtatg ttaaagcgtc tgactggggc
    20521 tggcagattg atccagtagg tctgcgctat gccctttgcg aactgtatga gcgttatcag
    20581 aggccgctgt ttattgtcga aaacggtttt ggcgcttacg acaaagtgga agaagatggc
    20641 agcatcaacg acgactaccg cattgactac ctgcgcgccc atatcgaaga gatgaaaaaa
    20701 gcggtgactt acgatggcgt ggatctgatg ggctacacac cgtggggctg catcgactgc
    20761 gtatcgttca ccaccgggca gtacagcaaa cgctacggct ttatctatgt gaataaacat
    20821 gacgacggtg ctggcgatat gtcgcgttca cgtaagaaga gctttaactg gtaccaagag
    20881 gtgattgcca gcaacggcga gaagctttaa gtcgatgaag taccggatgc aatacttgtt
    20941 gcatccggtc agacaacttc cctgttttat ttcccgcccg ccaaatcgat aaaacttccc
    21001 gtgacgtaag aggctttatc acttagtagc cagacaatgg cctgcgcgac ctcttctgcc
    21061 tgtccaccac gctgcatggg gatgttcgac ttaacgcgat cgacgcgtcc aggctcgccg
    21121 ccgctggcgt gcatttcggt ataaataaac cctggccgca cgcagttaac gcggatcccc
    21181 tgcgcggcga cttccagcga tagtccggtg gttaacgtat caatcgcccc tttcgatgcc
    21241 gtgtaatcaa catattcccc tggcgaaccc aaccgtgagg ccaccgaaga gacattgacg
    21301 atagcgccgc cactgccacc atttttaagc gccatgcgtt ttaccgcctc gcggcagcag
    21361 agaaaatatc ccgtcacgtt ggtggaaagt actcggttga ttcgctctgc ggtaaggttt
    21421 tcaacggtgc actgggtaaa caagatcccg gcgttattga ccagcgctgc tagcggttca
    21481 tcgtgctgat cgattgctgt aaacatcgca acgacctggt tttcgtcgct gatatccgcc
    21541 tggagcacga atgctttgcc accggcttgc gttattaagt tcatcacttc ctgcgccgcg
    21601 tggaggtttt gctgataatt aaccgccacc gtataccctt cttgcgccaa cagtaatgca
    21661 gttgcccgcc cgatgccgcg actgccaccg gtcacaagtg ctatagccat ttgtttttcc
    21721 gagaaaaatt cattcgttga cgtatctttg agattgctaa caaactggct caatcttcaa
    21781 tgttggcacg cttttccccc tcgccctttc agggagaggg ccggggtgag ggtaaatatt
    21841 cgcgccagtg ccggcctgtt cccctcaccc tctccccaaa ggggcgaggg gactgtctga
    21901 gcacttttgt actttgtcat ctgactaaaa aggcgccgaa gcgcctttag aaaatagtcg
    21961 aatcagtgaa ttactggtat tcgctaatcg gtacgcagga gcagaacagg ttacggtcgc
    22021 cgtaaacatc atccagacgt ttcaccgtcg gccagtattt gtctgccaca cctgccggga
    22081 atactgcaac ctcacggctg tacggatgcg cccactcggc gaccagttcg ttctgaatgt
    22141 gcggcgcgtt caccagcggg ttatcttcca gcggccagac accggcttta acctggtcga
    22201 tttctgcgcg gatagccagc atcgcgtcga taaagcgatc cagttccact ttgctttcag
    22261 attcagtcgg ttcaaccatc agcgtacccg ccaccgggaa cgacatggtc ggcgcgtgga
    22321 aaccgtagtc gatcaggcgc ttggcaatat ccagctcgct gatgccggtt tcttctttca
    22381 gcgggcgaat atcgagaata cattcgtgcg ccacgcgacc gtcgcgaccg gtatacagca
    22441 ccgggaaggc atcctgcagg cggctggcaa tatagttggc gttgaggatt gccacctggc
    22501 ttgctttttt cagcccttct gcgcccatca tgcggatgta catccagctg attggcagga
    22561 tagaggcgct accgaacggt gccgcagaaa ctgcgccctg acgggttaac atgccttcga
    22621 tttgcaccac gctatgaccc ggtacaaacg gtgccagatg cgctttcaca ccgatcggtc
    22681 ccatacccgg accaccaccg ccgtgcggaa tgcagaaagt tttatgcagg ttgaggtgcg
    22741 aaacgtccgc gccaataaag cccggcgagg tgatgccaac ctgggcgttc atgttcgcgc
    22801 catcaaggta aacctgaccg ccgaactgat gcacgacttc acacacttca cggatcgttt
    22861 cttcatacac gccgtgggta gaaggataag tcaccatgat acaggagagg ttatcgcccg
    22921 cctgttccgc tttcgcgcgc agatcagtca gatcgatgtt gccgttttta tcacacgcca
    22981 caaccaccac ctgcattcct gccatatgtg cagaagcggg gttagttccg tgcgcagaag
    23041 ccgggatcag gcagatatcg cgatgccctt cgttgcggct ttcatgataa tgacgaatcg
    23101 ccagcaggcc cgcgtattcg ccctgtgcgc cggagttcgg ctgcatacaa acggcgtcgt
    23161 aaccggtcag tttcaccagc cagtcagcca gctgcgcaat catctgctga taaccttcag
    23221 cctgttccgg cggacaaaac gggtgcagtt cggcaaattc cggccaggtg attgggatca
    23281 tctcggcggc ggcgttcagt ttcatggtgc aggaacccag cgggatcatc gcctgattca
    23341 gcgccagatc tttacgctcc agcgagtgca tatagcgcat catttcggtt tcgctgtggt
    23401 agcgattaaa caccggatgg gtgaggattt cgtcgtcgcg cagcatcgca ggctggatag
    23461 agcggctgtc gtgagccacg tctttgtcca gcgtgtcgat gtccaggccg tggttatcgc
    23521 ccagtagtac gctgaaaagc tgcattacgt tttcacgcgt ggttgtttca tcaagggtga
    23581 tcccaaccgc gttcagaata tcgctacgca ggttgatttc agccgcttcg gcacgcgtca
    23641 gtacgcccgc tttgtcggcc acttccacac acaaggtgtc gaaatagtgc gcatggcgca
    23701 gtttcagacc tttttgttgc aggcccgccg ccaggatatc ggtcagacgg tgaatgcggt
    23761 tagcgatacg tttcaggcca atcgggccgt gataaacggc atacaggctg gcgatgtttg
    23821 ccagcagcac ctgggaagta caaatgttgg agttcgcttt ctcacggcga atatgttgct
    23881 cgcgagtctg catcgccatg cgcagcgcgg tattgccagc tgcatctttc gatacaccga
    23941 taatacggcc cggcattgag cgtttgtatt catctttcgc cgcaaagaag gccgcgtgtg
    24001 ggccaccgta gcccatcggc acgccgaagc gttgcgccga accaaaaaca atatccgcgc
    24061 cctgtttacc cggcgcagtt aacagcacca gcgccataat atcggcggcg acactgacca
    24121 caattttgcg tgatttcagt tcgctaataa gcgcggtgta gtcgtggatt tcaccggttg
    24181 tgccaacctg ctgtaacagc acgccgaaga cgtcctgatg gtcgagcact ttttgcgcgt
    24241 catcgacaat cacttcaaaa ccaaaggttt cggcacgagt acggaccaca tccagcgttt
    24301 gcggatgcac atcggaagcc acgaagaagc ggttggcatt tttcagtttg ctgacgcgtt
    24361 tcgccatcgc cattgcttcg gcggcagcgg tggcctcgtc cagaagagaa gcagaggcca
    24421 tatccagtcc agtcaaatcc agcgttacct gctggaagtt gagcagtgct tcaaggcggc
    24481 cctgggagac ttcaggttga tacggagtgt acgcggtata ccagcccgga ttttccagca
    24541 tgttacgcag gataaccggc ggtagctgca cggcggtgta acccatgccg atgtaagacg
    24601 tgaagcgttt attgcgactg gcaatagcct tgagttctgc cagtgcggcg tattcggtcg
    24661 ccggtgcgcc aacctgcggc ggggtcgcaa gctgaatatc tttcggcaca atctggccgg
    24721 tcagcgcgtt taacgattgt gcaccaacgg cattcagcat ttcttgctgt tgcgcggcgt
    24781 ccggtccgat atggcgttca ataaaagcgc cgctgttttc aagctggctt aacgtctgtg
    24841 tcatgagcga tggttcctga aacgtgcagt gaattgtgaa cctctctcct tacgaagaga
    24901 gtgagggtga ggcataaatt ttcctcaccc tgatcctctc ccgcagaaga ggaataaagc
    24961 cgttactcgt cttctaacaa tgcttcgtat gcggtcgcat ccagcagtga ttccagttcg
    25021 ctttcatcgc tggctttgat tttaaagatc cagccacctg catacggttc gctgttcacc
    25081 agttccgggg aatcgctcag tgcgtcgttt accgccacga tttcaccgct tactggcgca
    25141 taaatgtctg acgccgcttt tactgattcg gcaaccgcgc agtcatcgcc cgcgctaacc
    25201 gttgcgccca cttccggcag gtcaacaaac accatatcgc ctaacagctc ctgggcatgt
    25261 tcggttatac caacggtgta agtgccgtcg gcttctttac gcagccattc gtgttctttg
    25321 ctgtatttca gttctgctgg tacgttgctc atcaatcaat ctccaaaaaa gtaaatcacg
    25381 cgacggcttt accgttacgc acaaaaacag gtttcgtcac tttaaccggc atttcacggt
    25441 tgcgaatttg cacaatcgcc gtttcgccaa taccttccgg cacgcgtgcc agcgcaatgc
    25501 tgtaacccag cgtcggggag aaagtcccgc tggtgataat gccttcatgc tggttgccct
    25561 gcgcatcggt aaaacgtacc ggcagttcat tacgcagcac gcctttttcg gtcatcacca
    25621 gaccaaccag tttttctgtg ccatgctcac gctgcacttc cagggcttca cgaccgatga
    25681 agtcacgatc tgccggttcc caggcgatag tccagcccat gttggcggct aaaggagaaa
    25741 tagtttcgtc catctcctga ccataaagat tcatgcccgc ttccagacgc agcgtgtcac
    25801 gcgcgcccag accgcatggc ttaacgcccg cttccaccag cgcacgccag aaatcggccg
    25861 ctttttcatt gggcagcgca atttcatagc ccgcttcacc ggtataacca gtggtggcaa
    25921 taaacagatc gcccgcctgc acgccaaaga acggtttcat cccttccacc gcctgacgct
    25981 gagcatcatt aaacagtgtg gcagcttttg cctgcgcatt cggcccttgt acggcgatca
    26041 tggaaaggtc atcacgaacg gtaatttcga tgccgaaagg ttcagcgtgt tgggtaatcc
    26101 aggagaggtc tttttcgcgg gtggcggagt taacaacgag gcggaagaaa tcttcagtaa
    26161 agtagtagac gatgaggtca tctatcacac cgccagaggc attcaacatc cccgagtaaa
    26221 gggctttgcc gcttttggtg agcttcgcca catcgttcgc cagcagataa cgcagaaact
    26281 cccgggtgcg gctgccgcga agatcgacga tggtcatatg tgacacatca aacattccgg
    26341 catcggtacg taccgcatga tgttcgtcga tttgcgaacc gtaatgcagc ggcatcatcc
    26401 agccgtggaa atccaccatg cgagcgccgc aaagcgtgtg ttgttcgtac aaaggagtct
    26461 gttgtgccat cttgtcctca ttgaataagc ggggctgaca actttttcat ggtgaaatta
    26521 tcaccacgaa acccagcatc ggagccactc ccggtcccca acgcaatcgt tctcttttgc
    26581 ctgaacttac caccgaaaca gactgttaac cataaggtaa aattgatcat cacattagct
    26641 tatggttaaa aaacgccaaa atcgcaacag aataaaaaac caaaaaatac acaagttttt
    26701 atataaagat gatgtgatga gaaagttaat ttgaataagg caatattaag agctgaaaaa
    26761 atgtcaaaaa acactaaatc aaaaattcgt gacattagaa aatataatgc gaaaacggag
    26821 gtgaaattag tttatttcaa atgaggaaaa tctcccggcg aaaaaaccgg gagatgaaag
    26881 tgtgatgggt atcaaataaa cagcagaaga gaaattttta acgcagccat tcaggcaaat
    26941 cgtttaatcc cattgcctgg cggataagtt gtggtttaac gccaggaagc gtgtcggcca
    27001 gtttcaaacc aatatcacgc agcagttttt tcgccggatt ggtaccggaa aacagatcgc
    27061 ggaatccctg cataccagcc agcatcagcg ccgcactgtg cttgcggcta cgctcatacc
    27121 ggcgcaggta aatgtactgc ccgatgtctt tcccctgacg atgcaaccgt ttcagttcag
    27181 caatcagctc tgcagcatcc ataaagccga gatttactcc ctgccccgcc agcgggtgaa
    27241 tggtatgcgc ggcgtcgccc accagcacca gacggtgcgc ggcaaactgg cgcgcataac
    27301 gccccgtcag tgggaacacc tgacgcgcgc tctcaacctt gcataagccc aggcgattat
    27361 caaaagcgat atttaacgcg cgattaaatt catcttcact tgcctgctgc atccgctgcg
    27421 cttcctctgg cgacagtgac cagacaattg agcaaagatg cggatcgcta agcggtaaaa
    27481 aggccagaat gccttcgcca tggaaaacct gccgcgccac cgcatcatgc ggttcttccg
    27541 tgcgaatggt cgctaccagc gcgtgatgct gataatccca gaaagtcagc ggaatatctg
    27601 ctttgttgcg caaccaggaa ttagcaccgt ccgcgccaat caccagacgc gccgttaaca
    27661 tgctgccatc tttcagcgtc aggaaagttt cattttctcc ccaggcgacc tgctgtaatt
    27721 ctgcgggggc caacagagtg atatctgacg actgctgcgc tttgttccac agcgcgtagt
    27781 gaatcactga gttttcaacg atatgcccaa gatggctata gcccatgctt tgatcgtcaa
    27841 acgaaatgtg accaaaactg tctttgtccc acacttccat accgtgataa caactggccc
    27901 tacgagagag aatgtcctgc cagacgccaa gacgggtgag taatttttcg ctggcggcat
    27961 tgatagccga aacgcgcagt tgtggtggtg catccgccgc cagaggttcc ggtacgcgct
    28021 gctccagtac ggcaacgcgt aagccgctcc cctgtaagcc gcaggcaacc gccagcccca
    28081 ccatgccgcc accaacaatg gctacatcaa cactttgcat tgtttattcc ttaaaaccgc
    28141 cttcaacgcg ccacccaacc gagggtgcgc tgcgccagca catcgcgtgc cggggtgaat
    28201 aattccatcg tcatcagtcc gatgttgcgc ccgacaacca gcggtgccca acggttggca
    28261 aaaagatgta ccaggctgtc cgtgacgccg atggttgctt cgcgatcact ctgtcgacgc
    28321 tgctgatagc ggcacaatat gccgtaatca ccgatgtctt ctccgcgctc atgcgcctga
    28381 gtcagggttt ccgcaaggct catgacatct cgcataccga ggttaaaccc ttgcccggca
    28441 atcgggtgca gagtttgcgc cgcattgccc accagcacgg tacgatgggt aatagctctg
    28501 gcggcgcggg ttaacgccag cggataagca ctgcgtttac cggcgtgggt aatttttcca
    28561 agtcgccagc caaaggccga ctggagttca cggcaaaact tctcgtcgct ccacgacagc
    28621 acctcttcgc gccgttccag tggatgacac cagaccagcg aacagcgtcc gtcagacatc
    28681 ggcaacatcg ccagcgggcc atgttgcgtg aagcgttcaa aagcgcggcc ttcgtgcgca
    28741 acggaagtcg caacgttggc aatcacggcc agttgttcgt aaggctcctg ctgccagtca
    28801 acgccgcacg cggtggctaa cgctgaatgg gtgccatcag ctgctaccag cacgcggccc
    28861 gtcagcgtct cgccactctc cagcgtcact tcaacgtgac tctgagtacg ggcaacgtta
    28921 gccacgcgat cagggcaatg cagcgttacg ccaggtgctt tacgcagcaa tgcaaacagc
    28981 cgttgcccga cattgtgcaa ttcgacaacc tgtcccagcg ccgccagttg gtaatcttct
    29041 gcggcgaggg tgacaaatcc agcgtgacca cgatcgctga catgcacggt agtgatggcg
    29101 gttgcgtaat ccgccagaga ttgccagacg ccgatgcgcg ccagttgctg acaggtcccc
    29161 gcagccagcg caatagctcg tccatcaaag cccggatgag catgtgactc tggcgcggtc
    29221 gcttcaatca aatgtaccgg cagcgccccg tgacttaacc gggaaatagc cagcgccagc
    29281 gtcgcgcccg ccatgccgcc accgacgatg attacgctca ttgctttctc gcagctgcca
    29341 tcaacgcttc gatttcttcc ggctttttca ccacgctggc ggtgaggttt tcgttgccgg
    29401 tttcggtaat cacaatgtcg tcttcaatac gaatgccgat accgcgatat tgttctggca
    29461 catccgcatc cggcgcgata tacagaccag gctctacggt cagtaccatc cccggttcca
    29521 gaatgcgcga gcgatcctga ccataaacgc caacgtcatg gacatctagc cctaaccagt
    29581 ggctaaggcc atgcataaag aaaggacgat gggcgttctg agcgatcagt tcatcaacat
    29641 cacctttcag aatgccgagt tttaccaggc cgctaaccat gatgcgcacc acttcaccag
    29701 tgacttccag aatggaagtt cccggacgat acaggcgcaa gctggtttcg agagactcca
    29761 gcacaatgtc gtagatttca cgctgggcct gagtgaattt gccgttgacc gggaaggtgc
    29821 gggtaatatc gccagcgtaa cctttgtatt cgcaacccgc gtcaatcaac accaggtcgc
    29881 cgtcgcgcat ttcacactcg ttttcggtgt agtgcagaat gcagccgttt tcaccgctgc
    29941 cgacaatggt gttataggac ggatagcgcg caccgtggcg gttaaattcg tggtgaattt
    30001 cgccttccag atggtactcg aacattcccg gacggcattt ttccatcgcc cgggtatgtg
    30061 ccatggcggt gatttctccc gcgcggcgga gtacggcaat ctcttctggc gatttgaaca
    30121 ggcgcatttc atgaacaaca ggacgccagt cgatcatcgt tgccggtgcg gtgagatttt
    30181 gccgcgaacc tttacgcagt ttttccagcg cactgttcac gatttcatca gcatatgcat
    30241 attcgccctg ggcatggtaa accacatcca ggccgttaag tagttgataa agttgctgat
    30301 tgatttcgct gaatgccagt gcgcggtcaa cgcccagttt ctctggcgcg gcatcctggc
    30361 ctaagcgacg gccaaaccag atctccgccg tcaggtcgcg aacgcggtta aacagaacgc
    30421 tgtggttatg agtgtcatcg cttttaatca gcaccagcac cgcttccggt tcgttaaagc
    30481 cggtgaagta ccagaagtca ctgttctgac gataggggta ttcgctgtcg gcgctacgtg
    30541 ttacttctgg tgcagcaaaa atcagcgcgg cgctgccggg ttgcatttgc tccaccaggg
    30601 cctgacggcg acgctgaaac tcttgccggg atatctcact cataacactc tccttacgtt
    30661 ttttgttttt agtgtagagt cggtttttgt acttctggcg cggtcggttg cggatgagta
    30721 aaggtgtcgt ggcataacag cgcggcaaca cgaacgtatt cgatgatctc ttcaagcgac
    30781 atttcaagct cttcctgatc ttcgtcttcg tcgtaaccca gttgcgcaat gttacgcaga
    30841 tcgtcgatag cttcaccggt ttcgccggtc actttatcca gcttcggttg cgtaacgcca
    30901 agaccaagca ggaagtgatt gacccaacct gccaatgcat cagcccgatc gaaaacgctg
    30961 acatcatcgc catcaggcag ataaagctga aaaaggaagc cgtcatcctg cagggcatcg
    31021 ctggtggcag agtgcatttt acgcagtgcc tgtgccagct catgaccgaa agccatgcct
    31081 tcgttcgtca ggtcgtgaag tagcggtagc catgagctgt catcgttacc gccacatatc
    31141 atcccactga ttaaaccatg catctcagca ggggtcagac ccgtcccttg ttggttcaga
    31201 tactggttca tttcgttgta accaggcatt tcgttctgta tagacataag cattcgtcgt
    31261 caaagggagg aatattcatg atatgctacc actttgggcc ctggtggacc agaaaagggc
    31321 ttgtctcttc tcatcagggt agctatagtg tcgccccttc gcagaccatg ggtctaaaga
    31381 cgaaggcagc gcagtcaatc agcaggaagg tggcatgtct gcacaacccg tcgatatcca
    31441 aatttttggc cgttcactgc gtgtgaactg cccgcctgac caaagggatg cgttgaatca
    31501 ggcagcggac gatctgaacc aacggttgca agatctgaaa gaacgcacta gagtcacaaa
    31561 tactgaacag ttggtcttca ttgccgcatt gaatatcagc tatgagttag cgcaagaaaa
    31621 agcgaagact cgtgactacg cggcaagtat ggaacagcgt attcggatgc tgcagcagac
    31681 catagaacaa gcgttacttg aacaaggtcg catcaccgaa aaaactaacc aaaactttga
    31741 atgacacttt tcggtttact gtggtagagt aaccgtgaag acaaaatttc tctgagatgt
    31801 tcgcaagcgg gccagtcccc tgagccgata tttcatacca caagaatgtg gcgctccgcg
    31861 gttggtgagc atgctcggtc cgtccgagaa gccttaaaac tgcgacgaca cattcacctt
    31921 gaaccaaggg ttcaagggtt acagcctgcg gcggcatctc ggagattccc ttcttatctg
    31981 gcaccagcca tgacgcaact accagaactc ccactgacat tatcccgaca agaaatccgc
    32041 aaaatgattc ggcaacgtcg tcgtgcgtta acgccggaac aacagcagga aatgggtcaa
    32101 caagccgcta cccggatgat gacttatccc ccggtggtga tggcgcatac ggtcgctgta
    32161 ttcctctctt ttgatggtga actcgacacc cagccgctaa tagaacaact ctggcgcgcc
    32221 ggtaagcgcg tatatcttcc agttttgcat ccctttagtg ccggtaattt gctgttcctg
    32281 aattaccatc cgcaaagcga actggtgatg aacaggttga agatccatga gccaaaactg
    32341 gatgtgcgtg acgtgctacc cctttcccga ttagacgtgc tgatcacacc gctggtcgcc
    32401 tttgatgagt acggtcagcg cctgggaatg ggcggtggtt tttatgatcg gaccttacaa
    32461 aactggcagc actataaaac gcaaccggtg ggttatgcgc atgattgtca gttggtggaa
    32521 aaactccccg ttgaagagtg ggatatccct cttcctgcgg tggttacacc gtcgaaagtc
    32581 tgggagtggt aagggcgata cacctgcatc gccctgattg acatcgttga ttctttaacc
    32641 taatttagtg agtaagggta agggaggatt gctcctcccc tgtgactgac tgttaataag
    32701 ctgggaaact tatgagtaac agtacaatca gtatgatggc aagtcgcatc ataacccttc
    32761 tccttcaagc cctcgcttcg gtgagggctt taccgttaca gccccatgct gcccagctat
    32821 cgtaagctct ctttaaataa ccacaacgca ttgatctacc tttaaatctt ttcttcgagc
    32881 gtgctcgaaa aaccccatgc aaaacagaga taatttttgg gaaaaaggat ctattacggc
    32941 agtaaacgtg ggcgatgaag tggctatcaa aaagttcggt tcagggcgat gcaccccgca
    33001 tcgccctgat tgacatcgtt gattctttga cctaatttag tgagtaaggg taagggagga
    33061 tgactcctcc cctgagactg actgttaata agctccgaaa cttatcagta acagtacaat
    33121 cagtatgatg acaagtcgca tcataaccct tctcctttaa gccctcgctt cggtgagggc
    33181 tttaccgtta cagccccatg ctgtcccgcc atcgtaaatc cccattaaat aaacacaacg
    33241 cattgatctg actttgattt attttctgga gcagactcgc aaagtagaat gcgcaacgcg
    33301 gcaacggtgt ggagaaggga taaaaaaacg ggcaagtcag tgacctgccc gttaattttc
    33361 agagaagggg aattagtaca gcagacgggc gcgaatggta cccggaatag ctttcattgc
    33421 ctgcagcgct ttttcggcaa cgtcttcgtc agcttcaata tcaataacta cataacccat
    33481 ctgggcggaa gtttgtagat attgcgcggc gatgttgacg ccctgctcgg cgaagatttt
    33541 gttcagcgca gttagcacgc ccggacggtt ttcgtggatg tgcatcagac gacgaccacc
    33601 atgcagtggc agcgagactt ccgggaagtt caccgcagag agcgttgagc cattgtcaga
    33661 atacttgatc aatttacccg caacttccag gccgatattc tcctgcgctt cctgagtcga
    33721 accgccaatg tgtggcgtca gaaggacgtt gtcgaattca cacagtggag aggtaaatgg
    33781 atcgctattg gtcgccggtt ccgtcgggaa tacgtcgatt gccgcccccg ccagatgttt
    33841 gctcgccagc gcatcacaca gcgccggaat atccaccaca gtaccgcgcg aggcattaat
    33901 cagcagcgag ccgggcttca ttagcgaaat ctctttcgcg cccatcatat ttttggtgga
    33961 cggattctct ggtacatgca gactcaccac atcgctcata ttcagcaggt cagaaagatg
    34021 ctgtacctga gtggcgttgc ccagcggcag tttgttttca atatcataaa agtaaacata
    34081 cattcccagc gattcagcca gaatgcccaa ttgcgtacca atatgaccgt agccgatgat
    34141 acccagcttt ttgccgcgcg cttcaaaaga acccgccgcc agtttgttcc acacgccacg
    34201 gtgcgcttta gcattggctt ccggcacgcc gcgcaatagc agcagcagtt cgccaatcac
    34261 cagctccgca acagagcgcg tatttgagaa cggtgcgtta aataccggga tcccgcgctt
    34321 tgccgccgca tccagatcaa cctggtttgt tccgatacag aaacagccaa tagcgaccag
    34381 tttttctgcg gcgttgatca cgtcttcagt cagatgggta cgggatcgca ggccgatgaa
    34441 gtgggcatcg cggatggatt cttttaattg ttcatcatcc agcgcgcctt tgtgaaattc
    34501 gatgttggtg taaccagctg cacgaaggct ttccagcgcc ttttggtgca cgccttctac
    34561 cagcagaaac ttaatcttgt ctttctccag cgataccttt gccatttacc caatcctgtc
    34621 ttttgaaatg ttgtgtgcgg atttgcatcc gcctttcaac atatcaaaaa ataatattgc
    34681 ggcaatatga acgtttgcgt cgcgatgttg aggaaatatc acgcaaaggt aattcttgga
    34741 tgaatatgct ggtttagagg atttatttag agcaatcgac aattgcctgg taaaagcgtg
    34801 acacatgtca ccaaatttaa tgaagagaat ttttttaacg ggggaggttc ccccgtcaga
    34861 tcatttcaca atggttttga caccgtcagg tgtgccaatc agcgcaacgt ccgcgccacg
    34921 gttagcaaac aagccaacag tcaccacgcc aggaatcgca tttatggcgt tttccatcgc
    34981 tatcgggtca aggatttcca tgccgtggac gtcgaggatc acgttgccat tatcggtcac
    35041 cacgccctga cggtattccg gacgaccgcc cagtttcacc agctgacgcg ccactgcact
    35101 acgtgccatc gggataactt ctactggcag cgggaattta cccagaatat caacctgctt
    35161 ggaagcgtct gcaatacaga taaatttttc tgcaaccgaa gcaatgattt tttcacgggt
    35221 cagcgccgcg ccgccgcctt tgatcatttg catgtggccg ttgatttcat ctgcgccatc
    35281 aacgtagatg ccaaggctgt cgacttcgtt gagatcaaaa acgtgaatgc cgaggctttt
    35341 cagtttttca gtggaagcat ctgaactgga aacggcccct tcaatctggc ctttcattgt
    35401 accgagcgcg tcaataaagt gtgcggcggt ggaacctgta cctacaccaa caatggtgcc
    35461 gggctgaaca tactgaagtg ccgcccatcc tactgctttt ttcaattcat cctgcgtcat
    35521 gatcgtttcg cctgtggtat gaaatttcac acgcattata tacaaaaaaa gcgattcaga
    35581 ccccgttggc aagccgcgtg gttaactcat ccataaaata tcgcgcaatg gcgggcatcc
    35641 cctttcgccc cgcaaataaa gcatacaacg gtctgggtat gccgctccac ggtgcgaaca
    35701 gaggcaataa cgccacgcca ttcaacacaa ccagggcgcg aactcgctga gcgaaatgca
    35761 ttagcgcaaa tccctgatct tttaagctgc gcgtttaacc acgaatacaa atgttacccg
    35821 ccgacggctt cggtatatgc aacctgacac aaaattgtgt catagtgcag gaaaaagcat
    35881 ttaccaggag cagacaacag caatgaaacg cccggactac agaacattac aggcactgga
    35941 tgcggtgata cgtgaacgag gatttgagcg cgcggcacaa aagctgtgca ttacacaatc
    36001 agccgtctca cagcgcatta agcaactgga aaatatgttc gggcagccgc tgttggtgcg
    36061 taccgtaccg ccgcgcccga cggaacaagg gcaaaaactg ctggcactgc tgcgccaggt
    36121 ggagttgctg gaagaagagt ggctgggcga tgaacaaacc ggttcgactc cgctgctgct
    36181 ttcactggcg gtcaacgccg acagtctggc gacgtggttg cttcctgcac tggctcctgt
    36241 gttggctgat tcgcctatcc gcctcaactt gcaggtagaa gatgaaaccc gcactcagga
    36301 acgtctgcgc cgcggcgaag tggtcggcgc ggtgagtatt caacatcagg cgctgccgag
    36361 ttgtcttgtc gataaacttg gtgcgctcga ctatctgttc gtcagctcaa aaccctttgc
    36421 cgaaaaatat ttccctaacg gcgtaacgcg ttcggcatta ctgaaagcgc cagtggtcgc
    36481 gtttgaccat cttgacgata tgcaccaggc ctttttgcag caaaacttcg atctgcctcc
    36541 aggcagcgtg ccctgccata tcgttaattc ttcagaagcg ttcgtacaac ttgctcgcca
    36601 gggcaccacc tgctgtatga tcccgcacct gcaaatcgag aaagagctgg ccagcggtga
    36661 actgattgac ttaacgcctg ggctatttca acgacggatg ctctactggc accgctttgc
    36721 tcctgaaagc cgcatgatgc gtaaagtcac tgatgcgtta ctcgattatg gtcacaaagt
    36781 ccttcgtcag gattaatcca tcaaataatg cctgatagca catatcaggc gttgtcctca
    36841 cttctttttg tattccttga atcacatcac aaaatagaca aatctcaggc ggcaaaaaac
    36901 gacgtctgaa tgcatttttt ttgctggcga caaacccacg taaaaagctc accgtaggcg
    36961 caaataccct cattttgatt gcgttttacg gagcaaataa tgtctaacgt gcaggagtgg
    37021 caacagcttg ccaacaagga attgagccgt cgggagaaaa ctgtcgactc gctggttcag
    37081 caaaccgcgg aagggatcgc catcaagccg ctgtataccg aagccgatct cgataatctg
    37141 gaggtgacag gtacccttcc tggtttgccg ccctacgttc gtggcccgcg tgccactatg
    37201 tataccgccc aaccgtggac catccgtcag tatgctggtt tttcaacagc aaaagagtcc
    37261 aacgcttttt atcgccgtaa cctggccgcc gggcaaaaag gtctttccgt tgcgtttgac
    37321 cttgccaccc accgtggcta cgactccgat aacccgcgcg tggcgggcga cgtcggcaaa
    37381 gcgggcgtcg ctatcgacac cgtggaagat atgaaagtcc tgttcgacca gatcccgctg
    37441 gataaaatgt cggtttcgat gaccatgaat ggcgcagtgc taccagtact ggcgttttat
    37501 atcgtcgccg cagaagagca aggtgttaca cctgataaac tgaccggcac cattcagaac
    37561 gatatcctca aagagtacct ctgccgcaac acctatattt acccaccaaa accgtcaatg
    37621 cgcattatcg ccgacatcat cgcctggtgt tccggcaaca tgccgcgatt taataccatc
    37681 agtatcagcg gttaccacat gggggaagcg ggtgccaact gcgtgcagca ggtagcattt
    37741 acgctcgctg atgggattga gtacatcaaa gcagcaatct ctgccggact gaaaattgat
    37801 gacttcgctc ctcgcctgtc gttcttcttc ggtatcggca tggatctgtt tatgaacgtc
    37861 gccatgttgc gtgcggcacg ttatttatgg agcgaagcgg tcagtggatt tggcgcacag
    37921 gatccgaaat cactggcgct gcgtacccac tgccagacct caggctggag cctgactgaa
    37981 caggatccgt ataacaacgt tatccgcacc accattgaag cactggctgc gacgctgggc
    38041 ggtactcagt cactgcatac caacgccttt gacgaagcgc ttggtttgcc taccgatttc
    38101 tcagcacgca ttgcccgcaa cacccagatc atcatccagg aagaatcaga actctgccgc
    38161 accgtcgatc cactggccgg atcctattac attgagtcgc tgaccgatca aatcgtcaaa
    38221 caagccagag ctattatcca acagatcgac gaagccggtg gcatggcgaa agcgatcgaa
    38281 gcaggtctgc caaaacgaat gatcgaagag gcctcagcgc gcgaacagtc gctgatcgac
    38341 cagggcaagc gtgtcatcgt tggtgtcaac aagtacaaac tggatcacga agacgaaacc
    38401 gatgtacttg agatcgacaa cgtgatggtg cgtaacgagc aaattgcttc gctggaacgc
    38461 attcgcgcca cccgtgatga tgccgccgta accgccgcgt tgaacgccct gactcacgcc
    38521 gcacagcata acgaaaacct gctggctgcc gctgttaatg ccgctcgcgt tcgcgccacc
    38581 ctgggtgaaa tttccgatgc gctggaagtc gctttcgacc gttatctggt gccaagccag
    38641 tgtgttaccg gcgtgattgc gcaaagctat catcagtctg agaaatcggc ctccgagttc
    38701 gatgccattg ttgcgcaaac ggagcagttc cttgccgaca atggtcgtcg cccgcgcatt
    38761 ctgatcgcta agatgggcca ggatggacac gatcgcggcg cgaaagtgat cgccagcgcc
    38821 tattccgatc tcggtttcga cgtagattta agcccgatgt tctctacacc tgaagagatc
    38881 gcccgcctgg ccgtagaaaa cgacgttcac gtagtgggcg catcctcact ggctgccggt
    38941 cataaaacgc tgatcccgga actggtcgaa gcgctgaaaa aatggggacg cgaagatatc
    39001 tgcgtggtcg cgggtggcgt cattccgccg caggattacg ccttcctgca agagcgcggc
    39061 gtggcggcga tttatggtcc aggtacacct atgctcgaca gtgtgcgcga cgtactgaat
    39121 ctgataagcc agcatcatga ttaatgaagc cacgctggca gaaagtattc gccgcttacg
    39181 tcagggtgag cgtgccacac tcgcccaggc catgacgctg gtggaaagcc gtcacccgcg
    39241 tcatcaggca ctaagtacgc agctgcttga tgccattatg ccgtactgcg gtaacaccct
    39301 gcgactgggc gttaccggca cccccggcgc ggggaaaagt acctttcttg aggcctttgg
    39361 catgttgttg attcgagagg gattaaaggt cgcggttatt gcggtcgatc ccagcagccc
    39421 ggtcactggc ggtagcattc tcggggataa aacccgcatg aatgacctgg cgcgtgccga
    39481 agcggcgttt attcgcccgg taccatcctc cggtcatctg ggcggtgcca gtcagcgagc
    39541 gcgggaatta atgctgttat gcgaagcagc gggttatgac gtagtgattg tcgaaacggt
    39601 tggcgtcggg cagtcggaaa cagaagtcgc ccgcatggtg gactgtttta tctcgttgca
    39661 aattgccggt ggcggcgatg atctgcaggg cattaaaaaa gggctgatgg aagtggctga
    39721 tctgatcgtt atcaacaaag acgatggcga taaccatacc aatgtcgcca ttgcccggca
    39781 tatgtacgag agtgccctgc atattctgcg acgtaaatac gacgaatggc agccacgggt
    39841 tctgacttgt agcgcactgg aaaaacgtgg aatcgatgag atctggcacg ccatcatcga
    39901 cttcaaaacc gcgctaactg ccagtggtcg tttacaacaa gtgcggcaac aacaatcggt
    39961 ggaatggctg cgtaagcaga ccgaagaaga agtactgaat cacctgttcg cgaatgaaga
    40021 tttcgatcgc tattaccgcc agacgctttt agcggtcaaa aacaatacgc tctcaccgcg
    40081 caccggcctg cggcagctca gtgaatttat ccagacgcaa tattttgatt aaaggaattt
    40141 ttatgtctta tcagtatgtt aacgttgtca ctatcaacaa agtggcggtc attgagttta
    40201 actatggccg caaacttaat gccttaagta aagtctttat tgatgatctt atgcaggcat
    40261 taagcgatct caaccggccg gaaattcgct gtatcatttt gcgcgcgccg agtggatcca
    40321 aagtcttctc cgcaggccac gatattcacg aactgccgtc tggcggtcgc gatccgctct
    40381 cctatgatga tccattgcgt caaatcaccc gcatgatcca aaaattcccg aaaccgatca
    40441 tttcgatggt ggaaggtagt gtttggggtg gcgcatttga aatgatcatg agttccgatc
    40501 tgattatcgc cgccagtacc tcaaccttct caatgacgcc tgtaaacctc ggcgtcccgt
    40561 ataacctggt cggcattcac aacctgaccc gcgacgcggg cttccacatt gtcaaagaac
    40621 tgatttttac cgcttcgcca atcaccgccc agcgcgcgtt ggctgtcggc atcctcaacc
    40681 atgttgtgga agtggaagaa ctggaagatt tcaccttaca aatggcgcac cacatctctg
    40741 agaaagcgca gttagccatt gctgttatca aagaagagct gcgtgtactg ggcgaagcgc
    40801 acaccatgaa ctccgatgaa tttgaacgta ttcaggggat gcgacgcgcg gtttatgaca
    40861 gcgaagatta ccaggaagga atgaacgctt tcctcgaaaa acgtaaacct aatttcgttg
    40921 gtcattaatc cctgcgaacg aaggagtaaa aatggaaact cagtggacaa ggatgaccgc
    40981 cgatgaagcg gcagaaatta tccagcataa cgacatggtg gcatttagcg gctttacccc
    41041 ggcgggttcg ccgaaagccc tacccaccgc gattgcccgc agagctaacg aacagcatga
    41101 ggccaaaaag ccgtatcaaa ttcgcctgct gacgggggcg tcaatcagcg ccgccgctga
    41161 cgatgtactt tctgacgccg atgctgtttc ctggcgtgcg ccatatcaaa catcgtccgg
    41221 tttacgtaaa aagatcaatc agggcgcggt gagtttcgtt gacctgcatt tgagcgaagt
    41281 ggcgcaaatg gtcaattacg gtttcttcgg cgacattgat gttgccgtca ttgaagcatc
    41341 ggcactggca ccggatggtc gagtctggtt aaccagcggg atcggtaatg cgccgacctg
    41401 gctgctgcga gcgaagaaag tgatcattga acttaatcac tatcacgatc cgcgcgttgc
    41461 agaactggca gatattgtga ttcctggcgc gccaccgcgg cgcaatagcg tgtcgatctt
    41521 ccatgcaatg gatcgcgtcg gtacccgcta tgtgcaaatc gatccgaaaa agattgtcgc
    41581 cgtcgtggaa accaacttgc ccgacgccgg taatatgctg gataagcaaa atcccatgtg
    41641 ccagcagatt gccgataacg tggtcacgtt cttattgcag gaaatggcgc atgggcgtat
    41701 tccgccggaa tttctgccgc tgcaaagtgg cgtgggcaat atcaataatg cggtaatggc
    41761 gcgtctgggg gaaaacccgg aaattcctcc gttcatgatg tattcggaag tgctacagga
    41821 atcggtggtg catttactgg aaaccggcaa aatcagcggg gccagcgcct ccagcctgac
    41881 aatctcggcc gattccctgc gcaagattta cgacaatatg gattactttg ccagccgcat
    41941 tgtgttgcgt ccgcaggaga tttccaataa cccggaaatc atccgtcgtc tgggcgtcat
    42001 cgctctgaac gtcggcctgg agtttgatat ttacgggcat gccaactcaa cgcacgtagc
    42061 cggggtcgat ctgatgaacg gcatcggcgg cagcggtgat tttgaacgca acgcgtatct
    42121 gtcgatcttt atggctccgt cgattgctaa agaaggcaag atctcaaccg tcgtgccaat
    42181 gtgcagccat gttgatcaca gcgaacacag cgtcaaagtg atcatcaccg aacaagggat
    42241 cgccgatctg cgcggtcttt ccccgcttca acgcgcccgc actatcattg ataattgtgc
    42301 acatcctatg tatcgggatt atctgcatcg ctatctggaa aatgcgcccg gcggacatat
    42361 tcaccacgat cttagccacg tcttcgactt acaccgtaat ttaattgcaa ccggctcgat
    42421 gctgggttaa tggtttgtcc cgtcacgatc ttcagcaata tgctgaagca tcgtggcggt
    42481 ataacgatga tttctgattg aataaagata ttcctgcata tacatcggcc tgtgcggcgc
    42541 atcaaaatat ttcagcgttg ccggattcac cagtcgccag gcaaaatgcg ggatcaccgt
    42601 cagaaaatgt ccgcgctcca cggcgctgat tttggccata aaactgtagg gacgataaat
    42661 aatggtggga ttgatcccac aaggtcgcat attcgcatca atcattgctt cgaagttagc
    42721 ccggttttga aagcgcattt gtagccaggg cagctcctga agcagctctt gctcttgacg
    42781 ctcttcataa cggcgagaaa cgagaaagcc caatcgcagc ggtggtaatt cactgatagt
    42841 caggttttcc agttcctgaa ctcgtgcaga tacatgctgt ggggagataa taaaatctaa
    42901 ctgacggtca aaaagattat cgataacacc gttttcgctg aactcaactg gctgggcggt
    42961 cacgccttca tacttatctc cgagactaat caactgatcg aaaataattg tcggataagt
    43021 attatctatg ccaatcacaa tttctcgtga acgacgccct gagttatgga tttcattatc
    43081 aatcgcagaa agtcgttgat aaaccgggaa cagtttttga tacagttcct gaccggcttt
    43141 attgaggctg atattattat ctttgcgggt aaatagcgta tagccaattt gttcttccag
    43201 cgcggcaatg cttttaccaa atggcgaagc cgtcatatgg attttttctg ccgcccgggc
    43261 aatattattg gtttgcgcta ataaaatgaa attgcgcatc tttttcgaaa taaaaatgtc
    43321 catcccccct ccccgcatcc ttgtccgctt aaccgtttca gtcagcctat ccttgatgaa
    43381 accgcgagca aagataggtg attacgtcat ggttttacag aaaattacag aaaaaggagg
    43441 caatatcggg taaaggcatt agcccgacga atacgtcggg ctacaagtat tattgtgctg
    43501 caggtgtttt agcgggttgt tgatccacag gttctaactg gaagaccaca tcgacctgat
    43561 catcaaactg aatagcggcc tgctcgtaag tttcctgggc agacaccggc gcggcatcgg
    43621 ctttcatcat ccgcaccatt gggctgggct gatagttgga aacatggtag cgcacgctat
    43681 ataccggccc cagtttacga tgaaagccgt tcgccagttc ctgcgcctga tgaatcgcgt
    43741 tatcaatcgc tgccttacgc gctttgtctt tataggcatc cggctgcgcc acgcccagcg
    43801 acacagaacg aatttcgtta agacccgcct tcagcgcgcc atccagcaag gaattcagtt
    43861 tgtctaactg acggagcgtg acttccaccg ttctcacagc gcggtagcct ttcaggatac
    43921 ttttaccatc ctgataatca taatctggct gggtgcgtaa attcgctgag ctgatatctt
    43981 ttttcgcgat ctgattgagt tcaaggaagg aaatgtattg tgcgacgcgc tcatctgcct
    44041 gtttcttggc tgtagcggca tccttcgcgg ccacgttaac ttcaatcgca agagtggcaa
    44101 tgtctggcac cgcatccaca cttgccgtac cggaggtgac aatatgcggt ccatccggca
    44161 attcgttagc ctgcgctgcc atcccgctaa tacccattaa tgccgccagg gcgataactt
    44221 tgaacttcac gttagtctct ccgtgttaat tgacttgccc aaaattcagg acctctggag
    44281 gcaagcttag cgcctctgtt ttatttttcc atcagatagc gcctaactga acaaggcttg
    44341 tgcatgagca ataccgtctc tcgccagctg caaggctata aaccacatga cacatcccac
    44401 taccagattg ataatgcgct gtgattttgc cgtgcgcagg cgcggtgcca gccaggctgc
    44461 gagaatagcc agaccaaaga accacaggaa agaggcgcta attgtcccga gcgcaaacca
    44521 acgttttggt tcgacgtcaa gctgcccgcc aaggctgccc agtacaacaa aagtatccag
    44581 gtaaacatgc ggattcagcc aggtcactgc caacatggtg gcgataattt tccatctgcc
    44641 ttgcttcagg acttcggcac tagctaactc aatattacta ctcatcgctg ttttaaaagc
    44701 gccaaaacca taccacagca agaagactac gccgccccag gtgaccagcg ccagcaacca
    44761 cggcgactgc atcaataacg cgctaccacc aaaaatcccc gcgcaaatca ggaccaaatc
    44821 gctgatagcg caaagtaagg caatcataat gtggtactga cgacgaatgc cctgattcat
    44881 cacaaaagca ttttgtgggc cgagcggcag aatcatagct gccccaagag caagaccttg
    44941 aaagtaataa gaaaacacgt tggatattcc gaattaagta tctgatgcag agactatatc
    45001 gcgggattgt tattagcgga aattgataat attaattggc aatcagaata actaataaga
    45061 gagaggccag agagtattat ctggcctcag cgttgattac gcagctttgt cttctttcac
    45121 ccgcttaaag ttcacatcca tttgcgggta cgggaagctg ataccggcgg catcaaattc
    45181 acgtttaata cgctccagca catcccagta cacgttttgc agatcgccgc tgttgctcca
    45241 gacgcggacc acgaaattaa tcgacgatgc accaagttcg ttcaggcgca cagtcatttc
    45301 gcgatctttc aaaatgcgat cttcagactg gataatattg gtcaggatct gcttaacctg
    45361 atcgatatcg gaatcatacg ccacgccaat aataaattcg ttacggcgaa ctggctcgcg
    45421 ggagaagtta ataatatttc cggcaataat tttaccgttc ggaataacga taattttacc
    45481 gtctgcagta cgcatggtgg tggagaaaat ctgcacactc agcacagtac cggctacgcc
    45541 gccgaggtca acatattctc cggcacggaa cgggcggaac atgacaagta acacgccagc
    45601 ggccaggtta gaaagtgacc cctgcaaagc cagaccaaca gctaagcctg cggcaccgag
    45661 tacagcaatg actgacgctg tttgtacacc cacgcgtccc agggctgcga ttagcgtaaa
    45721 ggcgataata ccgtaacgga ctaatgcaga aagaaaatca gcaacagtgg catcgatttt
    45781 acgggagatc atcaggcgat tcaccgcgtt ggaaatcatc cgcgcgataa tcaaaccaac
    45841 gatgatgatc gcgagtgccg ccacgatgtt tactgcataa cttagcagca gcgcctggtt
    45901 agctaccagc cagcttcccg cgccgtttat gctatcgaca acattcaaat cttccattca
    45961 atattccttt tcaactgact caaaatggag aaatacactc cgccgcctta tgacgggcag
    46021 tctgacaagg gtaaacaata aagcataatt ttgccaaata gatcacagat ttctcatctg
    46081 aatcaccttg cttttggcga aaaaatcccc gcagagcggg gctcagacag atatcaatgt
    46141 gcgctttgtc atgccggatg cggcgtgaac gccttatccg gcctacaaaa acctgcaaat
    46201 tcaatatatt gcaggatacg tgtaggcctg ataagcgtag cgcatcaggc aattttgctt
    46261 ttggcgaaaa aaagacccgc agagcgggcc ttgagataag cagaaaggaa tgacttacag
    46321 aacgtcgatc gcgttcagtt cctggaatgc tttttccaga cgagcgatca tcgaagtctg
    46381 accggcacgc agccatacgc gcggatcgta gtatttcttg ttcggctgat cttcgccttt
    46441 cgggttaccc agctgaccct gcagataagc ttcgttcgct ttgtagtagt tcagaacgcc
    46501 ttcccaggtt gcccattggg tatcggtatc gatgttcatt ttcactacgc cgtagcttac
    46561 ggagtctttg atttcctgag cagtagaacc ggaaccaccg tggaatacga agttcaggct
    46621 gttgtgcggc aggttgtgtt tcttagaaac atattcctga gaatcacgca ggatggtcgg
    46681 agtcagaacc acgttacccg gcttgtaaac accgtgtacg ttaccgaagg acgcagcgat
    46741 ggtgaaacgc gggctgattt tgctcagctc ggtgtatgcg taatcaacgt cttccggctg
    46801 ggtgtacagt gcagaagcgt ccatgtggct gttgtccacg ccgtcttctt caccaccggt
    46861 gcaacccagt tcgatttcca gagtcatgcc gattttggac atacgctcca ggtatttaga
    46921 gcagatttcg atgttctctt gcagagattc ttcagacagg tcgatcatgt gagaagagaa
    46981 cagcggctta ccggtagctg cgaagtgttt ttcacccgcg tccagcagac cgtcgatcca
    47041 cggcagcagt ttcttcgcgc agtggtcagt gtgcaggata accggaacac cataatgttc
    47101 agccatctgg tgaacgtgat gcgcaccaga gatcgcgccc aggatagcag caccctgcgg
    47161 aacgtcagat ttcacgcctt taccagcgat aaaggaagca ccaccgttgg agaactgaac
    47221 gataaccggc gctttaactt tagcagcagt ttccagtacg gcgttgatgg agtcagtacc
    47281 gacgcagttt actgctggca gtgcgaagtt gttttctttt gctacctgga aaactttctg
    47341 tacgtcatca ccagtgatta cgccaggttt tacgaaatca aaaatcttag acatgtctct
    47401 tgtcctgtat cgtcgggcct tatactcatc atacttcaag ttgcatgtgc tgcgtctgcg
    47461 ttcgctcacc ccagtcactt acttatgtaa gctcctgggg attcactctc ttgtcgcctt
    47521 cctgcaactc gaattattta gagtatgaaa aaatgcgtgc tctaaaagcg cgctgaaaca
    47581 agggcaggtt tccctgccct gtgatttttt acttcttagc gcgctcttcg agcatcgcta
    47641 ctgcaggcag tactttacct tccacgaatt cgaggaatgc gccgccgcca gtggagatgt
    47701 aggagatttt gtcagcaatg ccgaacaggt cgattgctgc cagagtgtcg ccgccgccag
    47761 cgatggagaa cgcttcgctg tctgcgatag cgttagccac gatttcagta cctttgcgga
    47821 agttcgggaa ttcgaacacg ccaaccggac cgttccacag aatggttttc gcattcttca
    47881 ggatttcagc cagttcctga gcggaagcat caccgatatc caggatctgc tcgtcagctt
    47941 tcacatcgtt aacagatttc agggtagccg gtgcagtttc agagaactcg gttgctacgc
    48001 gaacatcaga cggaaccggg atgttgcagg tggtcagcag acgtttagct tcgtcaacca
    48061 ggtcagcttc gtacagggat ttacccacat cgtggccttg tgccgcgata aaggtgttag
    48121 cgataccacc accaacaatc agctggtcag cgattttaga caaggagtcc agaacggtca
    48181 gtttggtaga tactttagaa ccaccaacga tagccaccat cgggcgagct ggttctttca
    48241 gtgctttacc cagcgcgtcc agttcagctg ccagcagcgg gcctgcgcac gcaacgtcag
    48301 cgaatttacc gataccgtga gtagaagcct gcgcgcggtg agcagtaccg aatgcgtcca
    48361 ttacgaacac gtcacacagt gcagcgtatt ttttggacag ggtttcgtcg tctttcttct
    48421 cgcctttgtt gaagcgaacg ttttccagaa caaccagttc accttcagca acgtcaacgc
    48481 cgtcgaggta atctttaacc aggcgaaccg ggttagacag tttgtctttc aggtagttaa
    48541 caaccggcag cagagagaat tcttcgttgt actcgccttc ggtaggacga cccaggtggg
    48601 aagttaccat cacttttgcg ccttgtttca gggccagttc gatggtcggc agagaagcac
    48661 ggatacgcgc gtcgctggtt actttcccgt cttttactgg tacgttcaga tccgcacgga
    48721 taaatacacg tttcccagca agatccagat cggtcatctt aattacagac atggtgaatc
    48781 ctctcgttga ttctaaaagt tttgcagacg ctgcttgcgt cttacctgaa agcaacagta
    48841 gccatagcta acgtcgtgtc gagcattcgg ttagcaaagc cccattcgtt atcgcaccag
    48901 accaacgttt tgatcaggtg tgcgccactg acccgggttt gggtgccatc gacaatggca
    48961 ctgtgcggat cgtggttaaa atctacagag accaacggca attccgtata gtcaactata
    49021 ccatgaaatg caccttgtgc tgctttttgc agcaacaggt tgacttcatt ggcttttaca
    49081 ggtttcttca ccgtcacgct taaatcgatt gccgtcacat ttatggttgg cacacgtacc
    49141 gcaatcgctt caaagcgatc gttaaattgc ggaaaaaatc gtgtgatacc ggcggccagt
    49201 ttagtatcga ccggaatgat cgactggctg gctgcccggg tgcgacgcag gtcaggatgg
    49261 tatgcatcaa taacctgttg atcgtgcatg gcggagtgaa ttgtggtcac agtgccggac
    49321 tcaataccgt acgcatcatc taacaatttg atgacgggaa ttatgcaatt cgtggtacag
    49381 gaagcgttag aaacgatgcg gtgttccgca cgaagttgat cctgattgac gccgtaaaca
    49441 acggtcgcgt cgagatcgtt actgccagga tgtgaaaaga gcactttttt ggccccggcg
    49501 gcaatatgcg cttcgccatg ctcgcgggag ccatatacgc cggtgcagtc gaggactaca
    49561 tcaacgccaa gttcacgcca ggggagcgat tgcagtgaac gttcatgcaa tacgcggatg
    49621 gcgtcatcac caacaaaaag ttgatcgcgt tcctgtcgta cttcccatgc aaaacggcca
    49681 tggctggtgt catatttcaa caaatgcgcc atgcccgcag catccgccag ttcgttgatt
    49741 gccaccacgg taatttccgc ccggcgtccg gattcataca aagcacgaac cacattacgc
    49801 ccgatgcgac cgaagccatt tatcgctacg cgtacggtca tagatctcct gcaaggtttt
    49861 ccctgagcaa atttgccaga cagagtaatc cagcaaatcg tccggggaaa ccttacctgt
    49921 cgcaaactgc gactgattgg ttaattgtcg aacatttaat cgactgaaac gcttcagcta
    49981 ggataagcga aacgtggaat aaaaggaatg attgtccagt caaagaaagc aattatctga
    50041 cttacgtcac gttttcagcc ccgcccctgt ggaatttatt ccactttgca gaatcacgaa
    50101 taagcgtagc aaaatctttt cgttgtgccg tcacaaaccc tgacttatat cagaatatga
    50161 ctgatgcagc gtcgatatta tccgcaatac gtccgagttt cggagtactc caatgatgac
    50221 attgccctac ccatattctt cctccgcacg cttttacgca ctgcgcctcc tccccgggca
    50281 ggaagttttt tcccagctac acgcttttgc acagcagcat caacttcatg ccgcctggat
    50341 tgcaggctgt accggtagcc tgactgacgt cgccttgcgc tatgccgggc aagaaggcac
    50401 cacgctcctg aacggtacat tcgaagtgat ctcactaaac ggcacgctgg aacagagtgg
    50461 tgagcatctt catctctgcg tttccgatcc acacggcacg atgcttggcg gtcatatgat
    50521 gcccggctgt accgtgcgga ccacgcttga actggtgatc ggtagccttg aggaactggc
    50581 gtttagccgc caaccttgcg cgctttctgg ttacgacgag ttgcatattt cccccgttaa
    50641 ataactactt tcaaagaggt tttcttatgg cgcgtagcca cttttccagt caggcactgg
    50701 tgttgatcgt tatatctatc gccatcaata tgatcggcgg gcaactggcc agcatggtca
    50761 aattacctat atttctcgac tccatcggca cacttattag cgcggtactg cttggtccgg
    50821 tgatcggaat gctgaccggc ttactgacca atttactctg gggattgctg accaacccga
    50881 tcgccgccgc atttgcaccc gtcgcgatgg tcatcggcct ggtcgccggt tgcctggcgc
    50941 gagcgggttg gtttcgcacc ctgccaaaag tcgttgtcag cggcgtgatt attacgcttg
    51001 cggtaacggt cgtcgctgta ccgctgcgta ctgcgctgtt cggcggtgtc accggtagcg
    51061 gagccgattt gttcgttgcc tggatgcact cgatggggca aaacctggtg gagtcggtgg
    51121 cgattacggt gataggcgcg aacctggtcg ataaaattct tacagcggtg attgtctggc
    51181 ttttactgcg ccagctgccg attcgcacca cgcgccattt tccggcaatg gctgccgtgc
    51241 gctaaatgca tccatttacc tcattaacac tgtgggcatt ggcggcctgc accacgctta
    51301 tcctgcccgc gcagaccatt ttgccgatct atagcgcggc gacctttttc tgccttattg
    51361 cgctaaaggc cacgcagcgg cgggcaaaat atgtcgtctg gctgatgttc tcgctgggag
    51421 ccgggctgtg gctggtacac ggtggctggc taccagaatg gctaagcggc acgccacgct
    51481 cgccagaacg gtggtcgcat gctattacgc tctggttgcg gattctggcg attgtttcaa
    51541 cctcacagtt gtggatgcag tacgtccccg tgcaatgttt tattcgcgct ctgtttgctt
    51601 ctcgcctgcc gccaggcgtc gcttatctgt ttgccgggcc gttattggtc gttgagcagt
    51661 taaaacggca attagcgatt attcatgaag cgcagcgtgc ccgcggcgtg ccgctggatg
    51721 agggctggta tcagcgttta cgtgcgatgc ccgcgttaat tattccgcta acacacaatg
    51781 cgcttaacga tctggctgtc cgtggtgcgg cgctggatat gcgggcattt cggatccaca
    51841 accgacgaac aacgttgtgg gcaccagccg atagcaccct acaacgcgta gcgcgttaca
    51901 ccatgatctt gctgatgctg acagaattcg gagcgtggat atggttacgt tagaacagtt
    51961 ccgttattgc ccgacacact caacgcatcc tccgttctgt tatgactttc actatgtcaa
    52021 accggggatg gtcgccatct ttggtgacaa cggcagtggc aaaagtacgc tggcacagct
    52081 gatggcgggc tggtatccgg actttctgcc aggagagata acaggcacgg ggacgctcct
    52141 cggtacgccc attgggcatc tcccgcttaa tgaacaatcc gccaccatcc agctcgtcca
    52201 gcaatcgccc tatttgcagc tctccggctg tacctttagc gtggaagaag aagttgcctt
    52261 tgggccagaa aatctgtgtc ttgctgaagc agagattatg gcgcggattg atgccgccct
    52321 gactctgacc gagtgccagc cgttgcgcca tcgtcacccg gcaacactct ccggtggaga
    52381 gacacagcgc gtggtcatcg cctgcgccat cgcgatgcag ccaaagctct taattcttga
    52441 tgaagccttt agccgcctga ctccgcaggc cagcgaaatg ctgttgcagc gcttacaaca
    52501 ctgggcgttt gaacgtggtt cgctgattat tctttttgaa cgtcatcgca cacctttcct
    52561 gaactactgc cagcaagcgt ggcaattgca aaacggagcg ctccagccac tatgttaacg
    52621 ttaaataaga tatcttatcg ctggcctggc gcggcaacgg attgtctttg cgacatttct
    52681 ctgcaactca aacaaggtga atggttggcg ctgaccggcg acaatggcgc gggaaaatcg
    52741 acgctgttac gagtaatggc aggtcttctt acgcccacag ccggtacggt tatgctgcaa
    52801 caacaggcga tgaaaaacct gaaaaaccgc cagcgcgcgg caaaggttgg cgtactgttt
    52861 caggaagcgg aaaaccaact ttttcacagc accgtcgctg acgagatcgc atttggtttg
    52921 aaactgcaaa aatgcccggc ggatgaaatc actcaacgta cccatgctgc cctgcaatgt
    52981 tgtcagttag cagacactgc caactcgcac ccgttggatc tgcatagtgc gcagcgacgg
    53041 atggtcgccg tcgcctgcct ggaagcactc tctccgccac tattgctgct ggacgagccg
    53101 agtcgggatt ttgacgaaaa ttggttgagc gtgtttgaaa gctggctgga gaagtgcggc
    53161 cagcgaggca cgagcgtcgt agcaatcagt catgacgccg catttacccg gcgtcatttc
    53221 tctcgtgtgg tgcgactgga agatggtcta atcaggaacg ttaatccacc agacgatatt
    53281 cacccgtagc ggtcatcatt aaggtcaaat ttgcaggcag actctcttcc agcccccggc
    53341 gaacattcgg cccgtcggta cggtcataaa aagcttctgc gtctgccagc gatacccctc
    53401 ccgccagctt gcgccctacc agtcgttccc gcaacgcagt agctggcgcg ttgataaaga
    53461 tcgagaaatc acaaaattgc gcaagctggc accacttctc gtcgtccagc aacaaccagt
    53521 ttccttcgac gatgacgagt ggtgcggtaa cgtgcaacgc atcttcaaca ggatcatgct
    53581 tttgtcgatc atactgcggc cacgtacaat ccccttccac tacctggcac agattttccg
    53641 ccagtttcgc aacgtcaaat gtctctggtg cgcctttgaa ggggcgcaat tgatgcgcat
    53701 ccagccagct attgtaatgg tgaaaaccat ccatcgggag cgtctggata gcgggtaatt
    53761 cagggtcttg ttgcgcgaga tattcccaga aggtagttaa ggtagattta cccgtccccg
    53821 gcggagcgca aagaaaaacc accgtacgtc gctgcggact taccgtctgt aaggccgcca
    53881 gcatacgcag caacggttta tgcacgcgtt ctatctcttc atcatggtac tgcgcttgca
    53941 cgttcagccc attcaccgtt aattcaattt tcacggcgtt aattccttta aggtggtcgt
    54001 tactgccagc gtcagcgccg ttttgaccat tccgctaaaa cgcgcctctg accagacggc
    54061 gaaatcggcg aacttcatcg cttttaacgc gtcatagagc gtcgcattgc aggttatgct
    54121 attgacgttg ctgataaaat cccaggtcat gtcatcggca aatccgggcg tttcatcctg
    54181 ttcgttcagg taatgccagt actgggaaaa gtgcgtgata tcggcataca gccatttatc
    54241 cattttcccc agcgcataaa tcaaacgcag cgcaacatca atatcgtcaa gcgggccgct
    54301 ttgggcgagt aacggcttca ctgcatattc gacgatctct tcatcgttat cgacaaacag
    54361 cgacggcaga aactggcgta tcccctggag caaaatagaa tgcgcagttt tcataaatga
    54421 aaataaattg tcctgtgcat ccagttgctc aagcacatca tcttctgtca gcgtcgccat
    54481 tggtcttccg atagatcacc atcaatgcag ggagtctatt atattacacg tttggtccgc
    54541 gccgccgatc agtaatgtct gcgtccgcac cccattcgcc gtctgttgga taccgttcac
    54601 caactcgccg cccgtcacgc ccgtagcgct aaagaggata tcgttccccc tcaccagttc
    54661 atcgagcgag tacacacggt taacgtcaac gcccattgcc ttacaacgct tgcgctcctg
    54721 ctctgcaatc tgccgatttt ccgtgtaatc ccctttagcc tgacaaaagt caatcagctc
    54781 ggcctgcata tccccaccta atgctttaac ggcacaggca gaaatcacgc cttctggcgc
    54841 accgccgatg gtgtacatca cgtcgtaagg attatcctgc cagcaggtca gcacgctggc
    54901 ggcgacatcg ccatccggca gagcgaaaac cttcacgccc agctgtgttg cttcttcaat
    54961 cgcggcactc aggcgcggtt tatccagcgt aaccatgcgc agcttgtcca gcggtttacc
    55021 taacgcccgc gccacgttac gcaagttgtc cgccagaggc aaagacagat caatcgcccc
    55081 ggcagccagt cgattaacaa ccagcttttt catatacata tcgggcgcat gtaacaggct
    55141 atctcgaggg gcgaaagcca tgaccgccag cgcattgctc tgccccatcg ctaccatccg
    55201 cgtgccttca atcgggtcaa ccgcgatatc gacttctggc ccatcgcctt tgcccacctc
    55261 ttcgccaatc cacagcatcg gcgcatggtc aatttctcct tcgccgataa ccactcgtcc
    55321 acgaaaagcg acgtcgttta atgcctggcg catcgcggtg accgccaggc catcaatttt
    55381 atttttgtcg ccacatccgg tttgcggcca ggcagccaac gctgcctgtt ccgtaacgcg
    55441 aaataatggc cacgccaggg acatcattgc gcggcctccc cgatatcaac gccaaattgt
    55501 gccagcagat atttctcggc ctgttcattc cagatgccgt gcgtttcctc aaccagccgc
    55561 gccaactctt taaacagcgg atcggttttg cctttttcgg caaaatcggc aatggcagtg
    55621 agcggcatgg tcacgccgtt ataaatgagt tttttaccgc cagggatatc cggcagattg
    55681 agcacggtat ctggcaccgc atccagccca ccgatatgcg tgaccataaa ggacggctgc
    55741 aactgccccg tggcgcttaa cgcaatcgcc tctttcatgt cgtccgttga accgccggat
    55801 gtaccgacta cgtgcgtgct attgtaatgg acgttgtaga aattaaacgg cactttgaag
    55861 tttttatccg tcggcccggc aaagaagttc agacagccat cttccgccag taattcgtca
    55921 gccatctcaa cgacagcagg caccgccgca taaacaaaaa tgtcatcgaa cccgacatct
    55981 cctgtcagcg cccgcagcgt ctggaccgga tcgctcatcc ctttggtatt cacatacacc
    56041 agctcaatgc ctttactggc cgccagatcc accggcagca gcttctgcac ctgcgccagg
    56101 cgtttgtcgt cgatatcgac caccaccacc cgtgacggtt gtatgccacc gttaatggcg
    56161 taatcgatag cgccaatgcc catcggaccc gcacacgcca gcagtgcaat attgccgcca
    56221 ggtttgacgc ccatgcgatg ctcataaaca tattgcgtgg tgtgataatt ggcatgataa
    56281 gcaccaataa tgcagcacat aggctctgcc agcgacgccg cagcaaaata agagccgtga
    56341 tacggtaata cgcagcccaa attaatggcg atttcgggaa taatcatata ggtggcgtta
    56401 ccgccaaaat attcgtagct gtagcctgct gaatatccgc ttggtaaccc catcgccggt
    56461 tgcaatacaa aacgctggcc ttttttatat ttgccagtga gatttttacc cacttcgaca
    56521 ataaccccgg cacattcatg cccggtaatg accggatgat tttctaaatc gtcgggtacg
    56581 cgtttatgtt cactaccgag taacgccgct ttccaggtcg ataaacagac gctgtcagaa
    56641 attacactca ctaacagttc attatcggta atttctggca gttcaaattc gcgcagacgg
    56701 acatcccgct tgccataaat agcagcaact ttggttttca tttttacctc tgtaatcagt
    56761 gtttatgttc ggcggttaat tgattaaata aagtgtcgag ttttggatcg ccaatatagt
    56821 tattaatcaa tatcagcggt ttatccgtca cacgtttcac gcgcccttcc agactggcgt
    56881 gagtgacgac gatatcggca tccgcaggca cgttttctat cgcgtaatgt tttacttcta
    56941 ttgccagccc cgctttttcc aggcgtttac ggaaggtggt cgcgcccatc gcactggagc
    57001 ccatccccgc atcgcaaaca aaggcgatac gcttaaccct gcttaacgag aatgcaccct
    57061 cttgtttcat cgccttaacc gcattggctg actgagcaaa ctcatcttcg ctctccgttt
    57121 ccaccgtttt ttccatcttc agtatcagcg aagtaatagc aaaggacacc agggtaccta
    57181 ccgtaacacc ggcaattgtc gccaggaacg agcctttcgg cgtcagtgcc aggtaagcaa
    57241 agatagaccc cggacttggg ccagccacca gaccaccgtc cagtaagtta aacatccagg
    57301 taccagacat accgcccgca atcatggcaa taatagtcag cggcttcatc agcacatatg
    57361 ggaaatacag ttcgtggatc ccaccgagga agtgaataat catcgccccg ggcgcagaac
    57421 gtttactcat ccctttacca aacaaggtaa acgccagcag cagccccagg cccggacctg
    57481 ggttagaggc caccataaag aagatggatt taccgttaac cgaagcctgt tgcattccca
    57541 gcggataata gacgccctga tcgatcgcat tattaaggaa aagcactttc gccggttcat
    57601 tgatgactga cagcagaggc agataacccg catgtaccag cgcctcgata cactctttga
    57661 cgaaagtatt ggcaattaac accgccgggc cgatcacttc aaaacccagc agacaaagga
    57721 gcatccccgc gatacctaat gagaagttat tgataaccat ctcaaaaccg gcaggtatgc
    57781 gtttttccag tgccttatcg acatatttta tgaccaaccc accgagcggc cccatgatca
    57841 ttgagccaag gaacatcggg atctctgcgc caacgatcac accaatagta cctattccgc
    57901 ccatgaccgc cccgcgttta ccgccgacca gatgaccacc tgtagaacca atcatcacgg
    57961 gcaataaata ggtaatcatc gggccgacaa ttttggcgaa atgttcgtta ggcagccaac
    58021 cggtgggaat aaataacgca gtaataaaac cccaggcaat aaaagcacca atattgggga
    58081 tgaccattgc agtcaaaaag cccccaaaag cctggacctt tgcacgagca gacttgtttt
    58141 ccataatatt atcctgtaga ggagagagta attatccgcg gctgataatg tccgctaatt
    58201 gtttttctga tgatgctgtt aagagttgtt cgagtatttc ttcttcgcac agtaattcac
    58261 ttaacgcctg aatagcgcca atgtgcgaat cggcatcggc agcagagagt ccgatgagta
    58321 atttaattgg ttcgtcattc cccggaaaat aaacaccttg ttcaagtaat gtcaaagaca
    58381 tcccggtttt aagcgcccca cattccggtc gcgcatgagg cattgccacg cctggtgcga
    58441 gaatataata agggccattg ttaatggtgg aatctttaat tgcctgaatg taattctcgc
    58501 tgatatagtt tttatccagc aatgatacca tcgagaaatc gatagcttcc tgccaatctt
    58561 ttgctgaatg tataactgag attgatgatt ctggaaaata atcactaagc cgcataaatt
    58621 atccttattt tatttttagg gtaccgtccg gcggtaaaaa taaaatactc gtgcggttta
    58681 atgaaagcaa ttgagcaggg aaaccgatgt tttgaaaaca gtgaattatt aatttcttat
    58741 ataacattga gttatagata taacaacaaa tgtcaatacg catatcgtga tgcatattac
    58801 ataattgata tttattgcat ataaatattt gtgatctaca acacgcttta tctattgctt
    58861 gtccgcaaac ggacatatca aggtaataaa aaaggtcgcc gaagcgacct ttttacccga
    58921 aatgctaatt acagcagttc ttttgctttc gcaacaacgt tatcaacagt gaagccgaac
    58981 tcttcaaaca gcagctctgc cggagcagat tcaccgaagg tggtcatacc gacgatagca
    59041 ccgttcaggc caacatactt gtaccagtag tcagcaatac ccgcttctac agcaacgcgt
    59101 gcagtaaccg ctttcggcag tacggattca cggtaagcag catcctgctt gtcaaatgcg
    59161 tcggtagacg gcatggacac cacgcgcgct ttcacgcctt cggcagtcag tttttcgtag
    59221 gcagcaacag ccagttcaac ttctgaaccg gtagcgatga aaatcagttc cggctgaccg
    59281 gcgcagtctt tcagcacata accaccgcgc gcgatgtttg ccagttgctc ttcagttcgt
    59341 tcctgctgcg ccaggttctg acgggagagg atcagtgcgg tcgggccgtc ctgacgctca
    59401 acaccgtatt tccacgcgac cgcggattca acctggtcac acggacgcca tgtagacatg
    59461 ttcggggtta cgcgcagaga agcgacctgc tctaccggct ggtgagtcgg gccatcttcg
    59521 cccagaccga tggagtcgtg ggtgtaaacc atcacctgac gctgtttcat cagcgcagcc
    59581 atacgtacgg cgttacgtgc gtattccacg aacatcagga aggtggaggt gtacggcagg
    59641 aaaccaccgt gcagggagat accgttagca atcgcggtca taccgaactc gcgaacaccg
    59701 tagtggatgt agttacccgc agcatcttcg ttgattgctt tagaaccaga ccacagggtc
    59761 aggttagacg gtgccaggtc agcagaaccg ccgaggaatt ccggcaacag cggaccgaaa
    59821 gcttcgatag cattctgaga cgctttacgg ctggcgattt tcgccgggtt agcctgcagt
    59881 ttagcaatga actcttttgc tttcgcgtcg aagtcagacg gcatttcgcc tttcatacgg
    59941 cgggtaaatt cagcggcttc ctgcggataa gctttcgcgt aagcagcgaa tttctcgttc
    60001 catgcggatt ctttcgcctg gcctgcttct ttcgcatccc actgagcata gatttcagac
    60061 gggatttcga acggtgcata tttccagccc agctgttcgc gggtcagggc aatttcagcg
    60121 tcgcccagcg gcgcaccgtg ggagtcgtga gtaccggctt tgttcgggga accgaaaccg
    60181 atgatggttt tgcacatcag cagggacggt ttgtcagtca ctgcgcgcgc ttcttctact
    60241 gcgcgtttga tggatgccgc gtcatgaccg tcgatgtcgc gaataacgtg ccagccgtaa
    60301 gcttcgaaac gcattgcggt gtcgtcggtg aaccagcctt caacgtgacc atcaatagag
    60361 ataccgttgt catcgtagaa cgcaatcagt ttacccagct tcagcgtacc cgccagagag
    60421 caaacttcgt gggagatgcc ttccatcatg cagccgtcgc ccatgaaggc gtaggtgtag
    60481 tggtcgacaa tgtcgtggcc cggacggtta aactgcgccg ccagcgtttt ttctgcaatc
    60541 gccataccta ctgcgttggc aataccctgc cccagcggac cggtggtggt ttccacacca
    60601 gcggtgtaac ccacttccgg gtggcccgga gttttagagt gcagctgacg gaagtttttc
    60661 agttcttcca tcggcagatc gtaaccggtg aggtgcagca ggctgtagat cagcatggag
    60721 ccgtggccgt tggacagcac gaagcggtca cggtcagccc aggacggatt ctgcgggttg
    60781 tgtttcagga aatcacgcca caggacttcg gcaatgtcag ccatacccat aggggcaccc
    60841 gggtgaccgg atttggcttt ctgtactgcg tccatgctca gcgcacgaat agcattggca
    60901 agctctttac gtgaggacat tttgactcca gatcggatga tgaagggcac gcccttaacg
    60961 acttgacgac agcgcgtttt gggctacgcc ggaaaatttg ccaacaattt accgcaagcc
    61021 gcgcgtcatg tacatggaac atccttttgc cgcttcagaa atctctggat catgctcgca
    61081 tgttgcgcaa tctactcgcc cgtccgctgc gcttttcctt atactgagac tgagcgtcga
    61141 ttcacctgca aacggcgcat ttttagaata atcctgacct tgtgcggaag agaaaacatg
    61201 aaaattcgcg ccttattggt agcaatgagc gtggcaacgg tactgactgg ttgccagaat
    61261 atggactcca acggactgct ctcatcagga gcggaagctt ttcaggctta cagtttgagt
    61321 gatgcgcagg tgaaaaccct gagcgatcag gcatgtcagg agatggacag caaggcgacg
    61381 attgcgccag ccaatagcga atacgctaaa cgtctgacaa ctattgccaa tgcgctaggc
    61441 aacaatatca acggtcagcc ggtgaattac aaagtgtata tggcgaagga tgtgaacgcc
    61501 tttgcaatgg ctaacggctg tatccgcgtc tatagcgggc tgatggatat gatgacggat
    61561 aacgaagtcg aagcggtgat cggtcacgaa atggggcacg tggcgttagg ccatgtgaaa
    61621 aaaggaatgc aggtggcact tggtacaaat gccgtgcgag tagctgcggc ctctgcgggc
    61681 gggattgtcg gaagtttatc tcaatcacaa cttggtgatc tgggcgagaa attagtcaat
    61741 tcgcaattct cccagcgcca ggaagcagaa gccgatgatt attcttacga tcttctgcgc
    61801 caacgcggca tcagcccggc aggtcttgcc accagctttg aaaaactggc aaaactggaa
    61861 gaaggtcgcc aaagctcaat gtttgacgac catcctgcat ccgccgaacg cgcccagcat
    61921 attcgcgatc gtatgagcgc ggatggggtt aagtaagtca gacataccga tctacaacat
    61981 aattgtaggt cggtaacgcc tgaatccccg atcagagtag tgatttcact ttatttagca
    62041 aaacggctac atcatccggt cgggtatatc ccgtatcagg gtctataatc gagctgtaaa
    62101 catgtggcat aatgcgcggc acccccgcct ccagacaagt tttcagaatc acactgaagt
    62161 tatccaaatc aatcccgcca gtcggctcaa tcagcgtcat accattgcgc gccgccgtgg
    62221 tcgccagcgc gtatagctcc ggaagcgacc tctcgccgcc catcggaaag aacttcgccg
    62281 cgtgtgcgcc catatctagc atcattcgca ccgccgtatc gcaggagacg cgggcaggcg
    62341 taccctggct gctgctaacg ccggtggaaa tcagcacttc gcccggcgtc cccgtcgggc
    62401 taaccagcgc gttgacatgg gtctgctcac cgcccgtagc cgcaagcgcg cctgccgcga
    62461 aaccacaacc ggtaaaggtc tggttaacgt gtgctggatg aatctgcgcc gcaatcatcg
    62521 cagctttgta atactgcgcc gggtcacccg cccccagacc tactgaaatg gacggtattt
    62581 ccgccatcca acgtttaact tcctggatcc cctcatccac cgaggagaac tgcgcggaaa
    62641 gcacgccaat cactgcgtgg ccttcggcgg cgtcaaagat ttcacgggca ttggcgatat
    62701 ctttcgccag cacattaatt gccacgcgat tacgatagaa attaatcttt tgcatgatct
    62761 gcaatctcct tcagtcgcgc cacgataagt gccatttccc cttccgccac cgtacgcgga
    62821 tcaaggctaa aaacgccctg gtggaggtta taccgccgtg cataaatcgc gatatcgcca
    62881 ccacgcagtt gcgcttcgac gacgcgagcg tcaactccca attcctgcgc atttactcga
    62941 atacgaatac gccagattgc acgcccggct tcatcctgct caatatcagc cgttaacccg
    63001 tgaatcgcag aaatcgcttc aaccactggc tggagctgtt ccgcggtgat gactgcctgc
    63061 ccctggtgat aattctccag cgcgtaaacc agcccaacca tgttctcctt gccgattttc
    63121 atcgccctgg caatgccgtg atgctgcgct ttgcaggctg caatccagat ttttttacca
    63181 gtgataaacc cggaggtcgg cgcgttaaag gcttttgcgc cgctgtagac caccatatcc
    63241 gctccgctca ccacccaggc acgtaaatcc tcttcggcgg cggcatcgac aatcagcggc
    63301 aggttgtaca tctgcgcgac atgtgcgaag tcctcgatgt tcaacattcc cttctgcacg
    63361 cagtgatgcg acttcacata cagcagcgcg gcggtcgcgt cggtaatggc actctccagc
    63421 tgccagagag ccgccaggtt acttgaaccc acctccacaa cgcgcccgcc acctaagcgg
    63481 atcgcgctga ggatcgatgc accgtaatcg acgttatggc cgcgcagcat caccacttca
    63541 ttggccatgc cggagctatc cggcatgagc gccacccggg ccttgtcgcc gcgtgtaatg
    63601 gcggcggcga cagcgatggc gatccctgct gaggcacagg aggtaacgta gctatcctcc
    63661 gccccggtaa agcgagagac cagctcgccg gttttgtcca ccagctggtc aatctccacg
    63721 aaagaggatg ccgcgcgcgc ggtggcctgc atcacctccg gcgcgacgct ggagacgccg
    63781 agaatcgtca tcttgccgca ggcgttaatc acctgtttca gtcccagttg ttgatagata
    63841 ttctgcgtca tggcttacag cactcccagc agcgagcaga ccacgctcaa cgccacaatc
    63901 gacagcagaa tcgtggtgta acgcggaccc tttttcacca aatagaagta gatggcgaac
    63961 actgccgcca gcggcagcag gccgggcgcg atggagtcca gaatctgctg taccaccact
    64021 tcagagcctt tcagtgcgct aatttttaaa ggtgaggtta tcttgacaaa gctcgcggat
    64081 agcgcgccca tcatgataag ccccagtacg ttagcgccgt agataagctc cttgatgcgc
    64141 cctccctgca gcagactgat aatcgaatcg cgccccagcg tatagccctt gtgcaccatg
    64201 ccgtagctga ttgccagcgt aatcgccggg tagagaatca gcggcaaaat accgcccatc
    64261 gcgctgccat tggcggcgaa cgggataaaa atagcaatca acagcggcat caccgccgcc
    64321 cagatgatgg agtcgcccat cccggccagc ggccccatca ggccggtttt aatcccggtg
    64381 atagaggcat cgttaatcgc ctcgccgcgg gtcttttgct cttccatggc gatggagatc
    64441 ccctggatca ccgcgccaaa cgtctgctct gagttgaaga agttcagatg gcgttttaac
    64501 gcctccacct gctcctcctt ttgcggatag agctttttga taatcggcgt catcgaggcg
    64561 cagaaaatca ggctctgtaa acgctcatag gagctggaaa cttccgcgcc cagccagtag
    64621 ataaaccacg ctttggtgat atccgctttg gtcagcgccc cggactcccg cgcgcgctca
    64681 accagttcat gctgcattac gtcgtcgctc attatgccgc tccttcattt ttcgccaggc
    64741 ctttaatcag gaaggccacg caggtgccga aaatcgccat cgccatgatg tccaccttga
    64801 ggtacagcac cgcgaagaat ccgccgataa accacggcag caggctcttt ttaccaatca
    64861 ccataatggt gatggcgaag cccagcgccg ggagaatccc gcccatgatt tcaaacgagt
    64921 gggtcagcca gtgcggcatc agcttgagga aactttccac tacgttctgg ccgaagtagt
    64981 tggcggcgaa caccactggg aaacgtagta ccagccccag cagcgccggg tagagaaagg
    65041 cgcagcgcat aatggctgac atattggccg tttccgcgtg tttatcggcc atatgcaccc
    65101 aggccgcatt tagcgtgcga cgcaactggt cgaggaatac gccgataacg ccaaatggaa
    65161 tcgccagtgc aatcgccagg ttcgggtcca tattggcctt aacggcgata ggaatggcga
    65221 tacaggccgc cagcgccggg tctgacggca cattgccgcc tggcgttgag gtcacaccca
    65281 gatagaccaa ctgcatcccc gcgccgataa tcatcgccgc aggcatattg ccgagcagca
    65341 ggccgacaaa gaccgccacc accactggct gtaacagcat ggcggagaag gtatagccaa
    65401 ggcgcaaccg ggcaaaccag taatacagcc ccatcaggct tgcaaaaagt aaggtatcca
    65461 taatttgtta tcccatgttc agagggtcag aattttttca ggatatcgtc cagcgactgc
    65521 ggtttatctt ccgggatggt ctggaaaaca gccttaatgc cgcgactttt caaatcgtta
    65581 agaatgccaa cgtctttttc atccagcgtt atgttctgga acaccgcttt acggtttggc
    65641 ccgccgccta aaccgccaac ctgaatatca gccacgtcaa agccctgctg caccgcttcc
    65701 tgaatcgccg ccagcgacgg gaacagcacc agcacgttgc cgtcgcccag ctggttctct
    65761 ttccacgcgg ccgcgaagct ctggttgcta aagcagtcca ctttgatatt cggcggcgcg
    65821 gccatcaggt agatattttt cataaacgga tcggcgtcca gctcgtcgct gaccaccgca
    65881 atacggttgg cctgagactg ccctacccac ttcgtcacca cctgtccgtg gataagacgg
    65941 ctgtcaatgc gacataacac gatgtttgcc atacgatttt ccttactgtt gtaattgacg
    66001 aatatgggag acgacgtcca ggcaactgtt gcgcccagct tccaccagat cggccacgca
    66061 ggcggtcagt gggccatgtt cgcgcttgtc cagcgcctcc agcagcagcg aggcattaag
    66121 cccggagatc accgccaccg gataatcggc gctcagccgc gccgccacgt tagaggtggt
    66181 gccgccgata aaatcggtca cgataagcga accttccggc atggttttca ccacggcttc
    66241 gacgcgctgg taaaactcgc ccagcgtatc gacgggcatc agcgcgattt ccgtcacgcc
    66301 ttcgatttcg cccatcacca tgcgcagact gttgcagagc tgcagccccc agccgccgtg
    66361 agttagcagc agaatttcag gtaacggatg aataggtaca gttgtggtca aaatagcctc
    66421 ctttgctcca gtgtttacat ttcatcagga gcaaaggacg tgccagaatt tgtgtcacgc
    66481 acagaggaaa tgagattgga tggggcgtta agccccgtaa accaggggat cagctgtaga
    66541 gcagttcgta gatataaaaa tattctgcgt ccgataaacg gatagcgtaa gcctcttcaa
    66601 tcggcaaaaa ggaggatttg atgacgctaa aggcacgagc gtccagatca agccggtttt
    66661 ctagcgccat ctgtaacggt ttgcggttaa tcacgatacg ctcgaccatg cagcagcagt
    66721 ggatcagaaa acgtagtgtc acctggcggc tcggtttcag ggagagcgtg ttggttaagt
    66781 agttcagaac atcctcgatc tctttcagaa ttcgctgcgg gttgagcacc gaaatatggt
    66841 tgatgatgct ctccatggtc agcgcgctga taaagcgcat ggcgctgcgc tccatctcca
    66901 gtcggcgctc gctgcttgag agatccggcg tcagcaggct gagcaccagc tccggccctt
    66961 gctcggaaaa cagttcctcc agcgagataa acggtatatc cggcagtccc ggctggaagg
    67021 tgccgacgat ccctgccagc cgttcactgg cattaagcgc ctgctgaata cgctccaggc
    67081 tgcgcacctc gttatagtcg agaatcacca tacgcgtgtc ctgcgacatc agctcgccaa
    67141 agctctcctc cagcaccttt ttgatctttt ccgccgtccc cattccggtt atacatgaga
    67201 taaccagcac tttgccaccg ctctcttgct gcggcgtgca gagccgacaa gggatatttt
    67261 tactctgcat aagcacagcc aattgcgata gatcgccagt ttcgtagctt aaatccagcc
    67321 caacctccag caggctggtc agagtgatat ttggcaacaa cagaacgtca atctggaaca
    67381 gcttgctgat ggtgctgcca aaatggacca gcgatccgat atccaccatc agtatcagcc
    67441 gctgccagca ccggtcctga atcatccggg tcagggtttc cagggtgtca tgcaccgact
    67501 gctcaaatgg catatcaatt gcgtagaaca gatcgcgctc cagcacccgg ttcacatatt
    67561 gcgcctggct cgttgcggtg gtcgcaccgt gagcgattag aatcacgccg caatccgggc
    67621 tggtgtcgat gcgctggcgg tagtggcgac actctttcag gaacaggcac agccagacca
    67681 cctccgtcgc cgggcactga atatgcaaca actcattgat tttgcggcac agaagcgtgg
    67741 cattgtcata ctcatcttta cagcgatcca gaatcaggct ggatgaatag agttgaggta
    67801 taagccctcg ccgcacatag ccaatcagcg caagaaagtg tttacgtaac gggttcgcca
    67861 ggttttctgg caatgtaaat cctaatacct gctccacgta accgatcagc agcgtcacac
    67921 gctcctcaat ttgatcgcca tagcgcggtg gatgcgccgc actatggcgg ctataaagac
    67981 catattcaaa aatcgaactg agtttgtttt tcaggatagc cagcgtttct gcgggcggca
    68041 cgttactgtt gcgcagattg acatattcac gagtcaggaa actgtaaaac aggtcgctct
    68101 cttctatttc cgcgctggtc gccaacgaat tttttaacgc gggcaacgta cgcgcatcca
    68161 cgttcagacg atctttaccg ccaaacaacg catcgaccag cagacgctgc tctggcgtgg
    68221 tattaaacga catctccgcc agtcgtttat ctaactctag cgtatcatta cgctcggtca
    68281 ttcccgacgc ccacgcctgc gcgcacaaga actgaatatc acttttaagc tggccgatat
    68341 tgccttccag gggtttactt aacaaccaat gcaagagcgt tttatcaagg cttatggtac
    68401 gttcaatttt acgactttca cgctgaaaga agcttacaat caattcgagc tgctcctcga
    68461 ccgaacgctg gcgaataccc ggtaagtcaa tacatacctg gatacgccgc tggaaggtac
    68521 gcaatagtgc cgagttgacc gactctgtag tggcgcaaat aaggcgcacc gaaattgaat
    68581 atgttggccc acttgaacca agtgggcggt attcaccttt atcgagaata gaaaagagct
    68641 tctcttgtcc ttcgtacggg aggcgatgca cttcatccag aagtaaataa cctccgtcag
    68701 cttgctccac caatcccgtt ttattctcac ttgccccggt aaatgccccc ttatggtgac
    68761 caaacagatg tgaggaaagc aactccggat tatgagcata ttcggcacag ttaaagtaaa
    68821 ccaacggagg tggcgaacct atggtgtgct tgcaagcgaa gcaatgcatt aactcagcaa
    68881 agaatgtttt gccaacacca gatgcaccgg ttaacagcac atgtagtccg tggggataaa
    68941 gcaccgctgc gcggcctttt tctaccgcat cacggagact acggtcgtat ccgataagtc
    69001 cggaaaacgg atcatcatct gtatattttt ctttctttgg caataaacta gccactgaat
    69061 gaactatgca ttcgcaatca tccagttttc gattaatgag caattctagt gcgtgacgat
    69121 gtagaaaaaa aacaggacgt ccctgacttt ttaccaccag gccttcattc cataattgat
    69181 taagatcttt actaaccgag ttgcgtgcca gcccaaggtt aaaaccaatc gcctcagcag
    69241 taaatgcttt ctccttcgct aaatcagaca gttccagccc atacgtcagc cgctccagct
    69301 ccttgtgaat aacatcaatc cgtctcattt ctttaccgct taaaaaacaa tagttgttca
    69361 gaacatacac tatttgtaaa cgtaagaatg agaactatcg ggaagaatta atgttccttg
    69421 tggcaatctg gtttctgaat attcgtgttt taaatattac ttatggtctg aaacaaccag
    69481 gcaggtgaac agagctaaga aaaagtagcg tagaaatata aaaacatata tcgcactgat
    69541 gaatgtttcc cggcatgact atgcacaccg ggaaatattt agtaaattac tcgccctttt
    69601 tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga gcagtgattt
    69661 ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg ttgagatctt
    69721 tcaggccgcg taccagttta atggcgcgat cggaggtcag accgccaatt accggcgtac
    69781 cggttcccgg cgcaaatgca ggatccaggc agtcgatatc aaaggtcagg taaaccggca
    69841 tatcacccac aatttgtttc acctgggcga taacgtcatc cacgctgcga tcgttcacct
    69901 ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ttcggtacga ataccaatct
    69961 gcacggaatg attcggatcg atcagacctt ctttcggcgc ggtatagaac atggtgccgt
    70021 ggtcaaattc acaaccgttc gcgtaggtat cggtgtgggc gtcaaagtgt accagcgcca
    70081 ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag tggtcaccac
    70141 cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc tgcagcttct
    70201 cgctcatctc acgggcatcg ccgaaagcat ataccagatc accgcagtcc acgacgttca
    70261 gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc agattcgtcg
    70321 aaacctgacg gatagctgcc ggaccgtggc gtccccccgc acgaccagaa gtggccatat
    70381 cgaacggcac accagtaatc acccagtcag catcgctgtc atacggctgg aagttcatcg
    70441 gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga tgacctaagg
    70501 tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg cgtcgttagg
    70561 cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc gccttaaatg
    70621 ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag ccattgttat
    70681 ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgcctgg ccttgtaatt
    70741 caaaggcggc aaagcctatt acaccggtat tacgcacatc gccttgcgcc gaattggctg
    70801 cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc ggtatgttta tctttgcgga
    70861 atcctttaat cgctaaagaa tctccggcat tcacaatata gccgttgtta ttcagcgaac
    70921 cttgcttgcc gttcagcaca tccaggccat caacagtagc aacgatctcg tagttggtgt
    70981 tacgactgta attacggaca tataattgat aattcattcc gactctggca tggacaatat
    71041 agcgtggttc attattggat gaatcataaa cacgcgtaat aggtagagaa ttaaaattac
    71101 catcgcgaat agcatattcc agttcgttaa tacgaatgct ataaacgcgg tcatagccgg
    71161 aaggaacatt agtcgaataa ctaataacga tcatctctgc cggatcgcgc caggctcgct
    71221 cagcagatac gccctgaacc gatgagtgca catccccacc ccaggcagtg ccgacattgc
    71281 ccgatgcact gctattatac tgcgcattac tggaacaacc ggccagcagc acgattaatg
    71341 ccaccaacgc gctacgtact ttccattttt tcataaccgt tccctctgca tttaattaat
    71401 gcagcgattg tagggagatt atttaataag aaaagcagcg agattatttt taagaatata
    71461 taaaaagaaa gcgggccgat ttacgtaaat aaatcgaccc gccagcggaa taatcttaat
    71521 tattcatctt caagataggt ataaccgtac aaacctgcct cgaactcttc aaggaactgt
    71581 tgttgcagtt cagcatcaag atcggttttc ttcacttgat cgcggaactg ggttaacagc
    71641 gttttcggat cgagctgtac atattgcagc atgtccgcca cggtatcgcc ttcgtcagac
    71701 agttctactt ctacgctacc gtcagggaac acgaacacgt caaccgcttc ggtatcaccg
    71761 aacaggttgt gcatattgcc aaggatctcc tgatatgcgc cgaccataaa gaaaccgagc
    71821 atcggcggat tctctggatc gtactccggc attggcattg tcgtggcaat accatcacca
    71881 tcaatatagt ggtcgatagc accgtcagag tcacaggtaa tatccagcag cactgcgcga
    71941 cgttccggca cttgatccag cccttccagc ggcagaaccg ggaacaactg gtcgatcccc
    72001 catgcatccg gcattgactg gaacagcgag aaattgacgt acattttgtc cgccatacgt
    72061 tcctgcagtt cgtcgattat cggacgatgc gcacggtttt gcggatccag ctgcttttgc
    72121 acttcatggc acatgctcag atagagttgt tctgcccatg cacgttcttg caggctaaag
    72181 gtgccggaag agtagccgat atgaatgtcg tgcagatcca tctggctgtc gtgtaaccat
    72241 tcacgcagag aacggcgagt tcccggttcg tgcatttcct gccaggtttc ccacatgctt
    72301 tgcagcgcgc gcggcgcatc ttctgcaggc gcggtcggca ccgtgtattc gttacgttcc
    72361 acgccgatga tattagatac cagcacggtg tgatgcgcag tcaccgcacg acccgattcg
    72421 gtgattaccg tcggatgcgg cagaccgttt tcttcacacg catcaccaat cgcccagatg
    72481 atgttgttgg catattcatt gaggccgtag ttcaccgaac agtcggactg cgaacgggtg
    72541 ccttcatagt ccacgcccag accgccgccg acgtcgaagc actgaatatt gacgcccagc
    72601 ttgtgcagtt ccacatagaa acgcgcggat tcacgaacgc ctgtcgcgat atcgcgaata
    72661 ttcgccatct gcgaaccgag gtggaagtgc agcagttgca ggctgtcgag acgcccggct
    72721 tcacgcaggg tttcaacaag ttgcagtacc tgagtcgcag ccaggccgaa cttcgatttt
    72781 tccccgccgg aggactgcca tttacccgag ccctgcgaag ccagacgtgc acgcacgccc
    72841 agacgaggaa cgacattcag acgttctgct tcatccagca caatggcgat ttctgacatc
    72901 ttctcaatga ccaggtagac cttgtggccc atcttctcgc caattaatgc caggcggata
    72961 tattcgcggt ctttataacc gttgcagacg atgacgctac gggtcatgcc agcatgtgcc
    73021 agcaccgcca tcaactctgc tttcgagcct gcttccagtc ccagcggttc gcccgaatga
    73081 atcagggact caatcacgcg gcggtgctgg ttaactttga tcggataaac aaggaagtaa
    73141 tcgccgttat agccgtagga ttcccgcgca cgtttgaacg cggcgttaat ggaacgcaaa
    73201 cggtgctgca ggatctgtgg gaaacagaac agtgcaggca gacgctgacc ctgtgcttca
    73261 cgagttttca ctaactgcgc gagatcgacg cgagcttccg ggacgtccgg gtccgggcac
    73321 acgctgatgt ggcccagctc gttaacgtca tagtagttat tgccccacca ggcaatattg
    73381 taagtacgca gcatcttgct ggcttcctgg gagctcattg caacctcctg catggagcgt
    73441 agtacaccgt gttcgcccgc tgacgaaggc aaacccatag acatgtcgtc agacatagcg
    73501 aacctcaaat tattttatta agtgtaaaac agttaacgac tatcgcagcc tggaactgcg
    73561 ataacaaccc ataagcacat ggattttcca gcagtgtatg ctgacgctcc gactgcgcaa
    73621 ccggtttctt tttcataaca ttattaagca cataaccgaa cgtaagtgtg acagttcggc
    73681 gaaaccacga gaaaactctt gtttttacaa gagcgccctt gttcagtcct cagtaactgt
    73741 aaccagctct tgaatcctga gaagcgccga gatgggtata acatcggcag gtatgcaaag
    73801 cagagatgca gagtgcgggg aacgaatctt caccagaacg gtgagacagg ttaagcagca
    73861 gacaacggtt cattatttcg tatcacctcc acggccgcct gttaagacga acccacaagc
    73921 caaaactctg atttcaaccc ggctggaagt ggcaacacga aagaaacgtc gtgtgctttt
    73981 tatttaagcc gcgcgccgcg ttttataccc cacaatggca gaaaattgca aaagataaat
    74041 acgcagaatg ccggcattgt caggaaaaat ttccagccac gtttttaaca gaatgagaca
    74101 cgattcaaaa aaaagtggaa atcgggtgaa gaattgacct aaaatagcca tccagatgtt
    74161 aatccatcca taccgattaa cactcagact gccagtgttt ttaacttgca gagtcgtggt
    74221 aggatccgct accacagaaa atccacacaa cagtttgagc taaccaaatt ctctttaggt
    74281 gatattaaat atggcaaaac acctttttac gtccgagtcc gtctctgaag ggcatcctga
    74341 caaaattgct gaccaaattt ctgatgccgt tttagacgcg atcctcgaac aggatccgaa
    74401 agcacgcgtt gcttgcgaaa cctacgtaaa aaccggcatg gttttagttg gcggcgaaat
    74461 caccactagc gcctgggtag acatcgaaga gatcacccgt aacaccgttc gcgaaattgg
    74521 ctatgtacat tccgacatgg gctttgacgc taactcctgt gcggttctga gcgctatcgg
    74581 caaacagtct cctgacatca accagggcgt tgaccgtgcc gatccgctgg aacagggcgc
    74641 gggtgaccag ggtctgatgt ttggctacgc aactaatgaa accgacgtgc tgatgccagc
    74701 acctatcacc tatgcacacc gtctggtaca gcgtcaggct gaagtgcgta aaaacggcac
    74761 tctgccgtgg ctgcgcccgg acgcgaaaag ccaggtgact ttccagtatg acgacggcaa
    74821 aatcgttggt atcgatgctg tcgtgctttc cactcagcac tctgaagaga tcgaccagaa
    74881 atcgctgcaa gaagcggtaa tggaagagat catcaagccg attctgcccg ctgaatggct
    74941 gacttctgcc accaaattct tcatcaaccc gaccggtcgt tttgttatcg gtggcccgat
    75001 gggtgactgc ggtctgactg gtcgtaaaat tatcgttgat acctacggcg gcatggcacg
    75061 tcacggtggc ggagcattct ctggtaaaga tccatcaaaa gtggaccgtt ccgctgcgta
    75121 cgcagcacgt tatgttgcaa aaaacatcgt tgctgctggc ctggccgatc gttgtgaaat
    75181 tcaggtttcc tacgcaatcg gcgtggctga accgacctcc atcatggtag aaactttcgg
    75241 taccgagaaa gtgccttctg aacaactgac tctgctggta cgtgagttct tcgacctgcg
    75301 cccatacggt ctgattcaga tgctggatct gctgcacccg atctacaaag aaaccgcagc
    75361 atacggtcac tttggtcgtg aacatttccc gtgggaaaaa accgacaaag cgcagctgct
    75421 gcgcgatgct gccggtctga agtaatcttt cttcacctgc tttcaaaggc cagcctcgcg
    75481 ctggcctttt tcttttacct caccgcggtg gataggcatt cacgccgcat ccggcaaaaa
    75541 gctgcccgca caaaaacatc attcttcctc aacacgtttt atcgcaaatt atcatcatta
    75601 ctgaaaccga ttacaccaat aacaccagcc aaatatttgt aatattttca tattattatt
    75661 cagttttcac agttgttaca tttcttttca gtaaagtctt aattgcagat aacagcgttt
    75721 aatctatgat gatataactc aattattttc atgcacttaa accataacta agataaatgt
    75781 tagtgtaagc gattacactg atgtgatttg cttcacatct ttttacgtcg tactcaccta
    75841 tcttaattca caataaaaaa taaccatatt ggagggcatc atgcctgacg ctaaaaaaca
    75901 ggggcggtca aacaaggcaa tgactttttt cgtctgcttc cttgccgctc tggcgggatt
    75961 actctttggc ctggatatcg gtgtaattgc tggcgcactg ccgtttattg cagatgaatt
    76021 ccagattact tcgcacacgc aagaatgggt cgtaagctcc atgatgttcg gtgcggcagt
    76081 cggtgcggtg ggcagcggct ggctctcctt taaactcggg cgcaaaaaga gcctgatgat
    76141 cggcgcaatt ttgtttgttg ccggttcgct gttctctgcg gctgcgccaa acgttgaagt
    76201 actgattctt tcccgcgttc tgctggggct ggcggtgggt gtggcctctt ataccgcacc
    76261 actgtacctg tcagaaattg cgccggaaaa aattcgcggc agtatgatct cgatgtatca
    76321 gttgatgatc actatcggga tcctcggtgc ttatctttct gataccgcct tcagctacac
    76381 cggtgcatgg cgctggatgc tgggtgtgat tatcatcccg gcaattttgc tgctgattgg
    76441 tgtcttcttc ctgccagaca gcccacgttg gtttgccgcc aagcgccgtt ttgttgatgc
    76501 cgaacgcgtg ctgctacgcc tgcgtgacac cagcgcggaa gcgaaacgcg aactggatga
    76561 aatccgtgaa agtttgcagg ttaaacagag tggctgggcg ctgtttaaag agaacagcaa
    76621 cttccgccgc gcggtgttcc ttggcgtact gttgcaggta atgcagcaat tcaccgggat
    76681 gaacgtcatc atgtattacg cgccgaaaat cttcgaactg gcgggttata ccaacactac
    76741 cgagcaaatg tgggggaccg tgattgtcgg cctgaccaac gtacttgcca cctttatcgc
    76801 aatcggcctt gttgaccgct ggggacgtaa accaacgcta acgctgggct tcctggtgat
    76861 ggctgctggc atgggcgtac tcggtacaat gatgcatatc ggtattcact ctccgtcagc
    76921 gcagtatttc gccatcgcca tgctgctgat gtttattgtc ggttttgcca tgagtgccgg
    76981 tccgctgatt tgggtactgt gctccgaaat tcagccgctg aaaggccgcg attttggcat
    77041 cacctgctcc accgccacca actggattgc caacatgatc gttggcgcaa cgttcctgac
    77101 catgctcaac acgctgggta acgccaacac cttctgggtg tatgcggctc tgaacgtact
    77161 gtttatcctg ctgacattgt ggctggtacc ggaaaccaaa cacgtttcgc tggaacatat
    77221 tgaacgtaat ctgatgaaag gtcgtaaact gcgcgaaatc ggcgctcacg attaatctcc
    77281 tcaggcttcc tcccatcgcg gaggaagcca cctcttgcag tcatcttttc ttcgctctat
    77341 cctctgccgc tatgaaaaca tcccgtctcc ctatcgccat ccagcaggcc gttatgcgcc
    77401 gcctgcggga aaaactcgcc caggccaacc tgaagctagg gcgtaactac ccggagccaa
    77461 aactctctta cacccagcgc ggaacctccg ccggaacagc ctggctggaa agctatgaaa
    77521 tccgcctcaa tcccgttttg ctgttggaaa acagtgaagc ttttattgaa gaagtggtac
    77581 cgcacgaact ggcacatttg ctggtatgga aacatttcgg ccgcgtagcg ccacatggca
    77641 aagagtggaa gtggatgatg gaaagcgtgc tgggcgttcc cgcccgtcgt acgcatcagt
    77701 tcgaactgca atccgtgcgt cgcaacacct tcccctaccg ctgcaagtgc caggagcatc
    77761 agcttaccgt acgccgccat aatcgcgtag ttcgtggcga ggccgtctat cgctgtgttc
    77821 actgcggtga acagctggtt gcgaaataac catctgaact atcaggaact ttcctgatct
    77881 ggctgattgc ataccaaaac aactttcgct acgttgctgg ctcgttttaa cacggagtaa
    77941 gtgatgtacc gttatttgtc tattgctgcg gtggtactga gcgcagcatt ttccggcccg
    78001 gcgttggccg aaggtatcaa tagtttttct caggcgaaag ccgcggcggt aaaagtccac
    78061 gctgacgcgc ccggtacgtt ttattgcgga tgtaaaatta actggcaggg caaaaaaggc
    78121 gttgttgatc tgcaatcgtg cggctatcag gtgcgcaaaa atgaaaaccg agccagccgc
    78181 gtagagtggg aacacgtcgt tcccgcctgg cagttcggtc accagcgcca gtgctggcag
    78241 gacggtggac gtaaaaactg cgctaaagat ccggtctatc gcaagatgga aagcgatatg
    78301 cataacctgc aaccgtcagt cggcgaggtg aatggcgatc gcggcaactt tatgtacagc
    78361 cagtggaatg gcggcgaagg ccagtacggt caatgcgcca tgaaggtcga tttcaaagaa
    78421 aaagctgccg aaccaccagc gcgtgcacgc ggtgccattg cacgcaccta cttctatatg
    78481 cgcgaccaat acaacctgac actctctcgc cagcaaacgc agctgttcaa cgcatggaac
    78541 aagatgtatc cggttaccga ctgggagtgc gagcgcgatg aacgcatcgc gaaggtgcaa
    78601 ggcaatcata atccgtatgt gcaacgcgct tgccaggcgc gaaagagcta acctacacta
    78661 gcgggactct ttttgttaac ccctacccca cgcgtacaac cgcgtgggga gacgacgcgg
    78721 atttttaact atgcgtatcc cccgcattta tcatcctgaa ccactgacca gccattctca
    78781 catcgcgctt tgcgaagatg ccgccaacca tatcgggcgc gtactgcgca tggggccggg
    78841 gcaggcgttg caattgtttg acggtagcaa ccaggtcttt gacgccgaaa ttaccagcgc
    78901 cagcaaaaaa agcgtggaag tgaaggtgct ggaaggcaag atcgacgatc gcgaatctcc
    78961 gctgcatatt cacctcggtc aggtgatgtc gcgtggtgaa aaaatggaat ttacgatcca
    79021 gaaatcgatc gaactcggtg taagcctcat tacgccactt ttttctgagc gctgcggcgt
    79081 taaactggat agtgaacgtc tgaacaagaa gctccagcag tggcagaaga ttgcaattgc
    79141 tgcctgtgag cagtgtggtc gtaaccgggt gccggaaatc cgcccagcga tggatctgga
    79201 agcctggtgt gcagagcagg atgaaggact gaaactgaat cttcacccgc gcgccagtaa
    79261 cagcatcaat acgttgccgt taccggttga acgcgtccgc ctgctgattg gcccggaagg
    79321 cggtttatcg gcagatgaaa ttgccatgac tgcccgctat caatttactg atatcctgtt
    79381 gggacctcgc gttttgcgta cagagacaac tgcgctcacc gccattaccg cgctacaagt
    79441 acgatttggc gatttgggct aacggagaag aataatgatc aagctcggca tcgtgatgga
    79501 ccccatcgca aacatcaaca tcaagaaaga ttccagtttc gctatgttgc tggaagcaca
    79561 gcgtcgtggt tacgaacttc actatatgga gatgggcgat ctgtatctga tcaatggcga
    79621 agcccgcgcc catacccgca cgctgaacgt gaagcagaac tacgaagagt ggttttcgtt
    79681 cgtcggtgaa caggatctgc cgctggccga tctcgatgtg atcctgatgc gtaaagaccc
    79741 gccgtttgat accgagttta tctacgcgac ctatattctg gaacgtgccg aagagaaagg
    79801 gacgctgatc gttaacaagc cgcagagcct gcgcgactgt aacgagaaac tgtttaccgc
    79861 ctggttctct gacttaacgc cagaaacgct ggttacgcgc aataaagcgc agctaaaagc
    79921 gttctgggag aaacacagcg acatcattct taagccgctg gacggtatgg gcggcgcgtc
    79981 gattttccgc gtgaaagaag gcgatccaaa cctcggcgtg attgccgaaa ccctgactga
    80041 gcatggcact cgctactgca tggcgcaaaa ttacctgcca gccattaaag atggcgacaa
    80101 acgcgtgctg gtggtggatg gcgagccggt accgtactgc ctggcgcgta ttccgcaggg
    80161 gggcgaaacc cgtggcaatc tggctgccgg tggtcgcggt gaacctcgtc cgctgacgga
    80221 aagtgactgg aaaatcgccc gtcagatcgg gccgacgttg aaagaaaaag ggctgatttt
    80281 tgttggtctg gatatcatcg gcgaccgtct gactgaaatt aacgtcacca gcccaacctg
    80341 tattcgtgag attgaagcag agtttccggt gtcgatcacc ggaatgttaa tggatgccat
    80401 cgaagcacgt ttacagcagc agtaacccac cttagcgaga aggatctcgt tgagactctg
    80461 agtgacagcg cccttctttc cacgcatact gggcgctgtt gcttttttga accaggaaac
    80521 agaacctctg acaatgaatt tacagcatca ctttcttatt gccatgcctg ctctccagga
    80581 tccgattttc cgtcgttccg tggtctacat ttgcgaacat aataccaatg gtgcaatggg
    80641 gatcatcgtc aacaagccgc tggaaaatct caaaattgaa gggattctgg aaaagctgaa
    80701 gatcacgccg gagccgcgtg atgaatcaat ccgtctggat aaaccggtta tgctcggcgg
    80761 tccgctggct gaagatcgcg ggtttatttt gcatactccg ccctccaatt ttgcttccag
    80821 cattcgcatt tcagacaaca cagtgatgac cacttcccgc gatgtgctgg aaacgctcgg
    80881 caccgataaa caaccgtctg acgtattggt ggctctgggt tatgcctcct gggaaaaagg
    80941 tcaactggaa caagaaattc tcgataacgc gtggctaacg gccccggcgg atctgaatat
    81001 tctgttcaaa acgccgattg ccgaccgctg gcgcgaggcg gcaaaactga ttggtgtgga
    81061 tattctcacc atgcctggtg tggcagggca cgcctgatga gtggaacctt actcgccttc
    81121 gacttcggca ccaaaagcat tggcgtagcg gtcggccaac gcattaccgg cactgctcgc
    81181 cctttgcctg caattaaagc acaggacggt acgccggact ggaacctcat cgaacgttta
    81241 ctgaaagagt ggcagccgga cgaaatcatc gtcggtttgc cgctgaatat ggacggcacc
    81301 gagcaaccat tgactgccag agcgcgtaaa tttgccaacc gtattcatgg tcgtttcggt
    81361 gttgaagtaa agctccatga cgaacgtctt agcaccgtgg aagcccgttc cggtctgttt
    81421 gaacagggcg gctatcgggc gctcaacaaa ggcaaagtcg actctgcctc tgcggttatt
    81481 attctcgaaa gctatttcga gcagggatat taaggcgatc taaaagcctg gcggatagtc
    81541 gttaccgcca tcttgccgga tgcggcgtga acgccttatc cagcacacat ctggcagcgg
    81601 ctgtaggtct gataagacgc gttagcgtcg catcagacat ttattgcctt tataggccct
    81661 ataaacttgc gcatcgggca tggcaacgtc acaaacgccc ttcccccacc cgctgctgat
    81721 aactctgctg aaacgttaac atccccacct gctgcccggt ttgaataaca tgcggtaact
    81781 ggtgggtttt cccttcgcga atcaaattcc ccaccgcggg agtgttaatc aataattcaa
    81841 acagcgccac gcgtccttcc tgtttatcca cttccagctt ttgtgacaac acggcccgta
    81901 aactacctgc cagttgatta cgtacgggat ctttttcctg cgccggaaat gaatccacca
    81961 gtcgctcaac tgcctgcgcg gcaccacgcg tatgtaatgt tgccagcacc aaatgcccgg
    82021 tttctgccgc cgtcagtgcc agacggattg tctcgctgtc acgcagctct ccgagcaaaa
    82081 tcacatcagg atcttcccgc aatgcggccc gcaatcccga tgcgaacgtc atacagtgca
    82141 aaccaatttc ccgctgctgg atcaaacatc gctggctggc atagatatat tcaacaggat
    82201 cttccagcgt cagaatatgc gcatcggcat gttgatagag atagccaacc atcgccgcta
    82261 gcgtggtaga tttgccgctc cccgtcgccc ccgtcaccag aatcaggcca ttctcgctct
    82321 tgagtaattc cggcaatacc gttggtgcgc caagctgttc gagctgtgga cagtgcgaag
    82381 gtaacaaccg taatgccaga gaaatacctt gccgttgcgc gaacgcactg ccacgcaacc
    82441 gctggttttc cgccagcgac acggcaaaat ccagctggcc attttccagc aatattgccc
    82501 gctgatcgtc atccagccac tcccgcagta gctcttcgac gtccggcgca tcaaacggcg
    82561 cagcttccat tagcccgcga atgcgccatc gtgcggacca ggcgctgcac aggtgtagat
    82621 ccgagacgtt atgctttaca ctaagggcca caatttcttc catattcata ctaagatcct
    82681 cggaaaatga acgatattgc gcataacctg gcacaggtcc gggacaaaat ctcagcggct
    82741 gcaacgcgtt gcggccgttc tccagaagaa attacgctgc ttgcagtcag taaaacaaaa
    82801 cctgcgagcg ccatcgcaga agccattgat gccgggcagc gtcaatttgg tgaaaactac
    82861 gttcaggaag gggtagataa aattcgccac tttcaggaac tgggcgtaac aggattagaa
    82921 tggcatttta ttggcccgtt gcagtctaat aaaagccgcc tggtggcaga gcatttcgac
    82981 tggtgtcata ccatcgaccg tttgcgcatc gctacccgcc tcaacgatca gcgcccggca
    83041 gaacttcccc ctcttaacgt actgattcaa attaatatta gtgatgaaaa cagtaagtcc
    83101 gggattcaac tggcagaact ggacgagctg gcagctgcgg tcgctgaact accgcgttta
    83161 cgtctgcgcg ggctgatggc aatccctgca cctgagtcag aatatgtaag gcagtttgaa
    83221 gttgcacgcc aaatggctgt agcatttgcc ggactgaaaa cgcgctaccc gcatatcgac
    83281 acgctctctc tgggaatgtc ggacgatatg gaagccgcca ttgcggcagg tagcacgatg
    83341 gttcgtatcg gcactgcaat ttttggtgcg cgtgattact ctaaaaaata aggaattaaa
    83401 ggaacgccat gaatacgttg actttcctgc tttcaacggt cattgagctg tataccatgg
    83461 tgctgttatt acgcatctgg atgcagtggg ctcattgtga tttttacaac cccttctcac
    83521 agtttgtagt gaaggtaacg cagccaatta tcgggccact gcgccgcgtt attccggcaa
    83581 tgggccccat tgacagcgcc tcgctgctgg ttgcctatat tctcagtttt atcaaagcca
    83641 tcgtgctgtt taaagtggtg accttcctgc caatcatctg gattgccggt ttactgattc
    83701 tgctgaaaac catcggcctg ctgattttct gggtcctgct ggtgatggcg attatgagct
    83761 gggtaagcca gggtcgtagc ccgattgaat acgtgctgat tcagctggcc gatccgctgc
    83821 tgcgcccgat tcgccgcctg ctaccggcaa tgggtgggat tgatttctcg ccgatgatcc
    83881 tcgttctgct gctgtatgtc atcaatatgg gtgtcgcaga agtattacag gcaaccggaa
    83941 acatgctgct gccggggctg tggatggcgt tatgagtgcc gtaacagtta atgatgacgg
    84001 tctggtttta cggctctata ttcagccgaa agccagccgt gattctattg tcggtttaca
    84061 tggcgacgaa gttaaagtcg ccattaccgc gccgccggtt gacggtcagg ccaacagtca
    84121 tctggtgaag tttctcggta agcaattccg ggttgccaaa agccaggtgg tgattgaaaa
    84181 aggcgaactt ggccgccaca aacaaattaa aatcattaat ccgcaacaaa tcccgccaga
    84241 aatcgcggcg ttaattaatt aggtatccta tgcaaaaagt tgtcctcgca accggcaatg
    84301 ccggtaaagt gcgtgagctg gcgtcgctgc ttagcgactt cggtcttgat atcgtggccc
    84361 aaacagacct cggcgtagat tccgctgaag aaaccggcct gacctttatc gaaaacgcga
    84421 ttctgaaagc gcgccatgcg gcaaaagtga ccggtttacc ggcaattgcc gacgactctg
    84481 gtctggcggt agatgtgctt ggcggcgcgc cgggcattta ctccgcgcgt tattccggtg
    84541 aagacgcgac cgatctaaag aatctgcaaa aactgctgga aacactgaaa gacgtaccgg
    84601 atgaccaacg tcaggcgcgt ttccactgcg tgctggtata tctgcgtcac gcggaagatc
    84661 cgactccgct ggtatgccac ggtagctggc cgggcgtgat tactcgtgaa ccagcgggca
    84721 ctggtggctt tggttatgat ccaatcttct tcgtaccttc cgaagggaaa accgccgccg
    84781 aactgaccca cgaagaaaag agcgccattt cccaccgtgg tcaggcattg aaactgctgc
    84841 tggacgcttt acgtaatggt taaattaccg ccgctgagtc tctacattca catcccgtgg
    84901 tgcgtgcaga aatgcccgta ctgcgatttc aactctcacg cgttgaaagg agaagtgccg
    84961 cacgacgatt acgttcagca tctgctttgc gatctggaca acgatgtggc ttacgctcag
    85021 ggccgtgaag taaagacaat ctttattggc ggtggtacgc cgagcctgct ttccggcccg
    85081 gcgatgcaaa cgctgctgga cggcgtgcgt gcgcgtttgc cgctggcagc ggatgcagaa
    85141 attactatgg aagcgaaccc tggtacggta gaagccgatc gctttgtcga ttatcagcgt
    85201 gctggtgtga accgcatctc tattggtgtg cagagtttta gcgaagacaa gctgaaacga
    85261 cttgggcgta ttcatggccc gcaagaagcg aaacgcgcgg caaagctggc gagcgggctg
    85321 gggctgcgta gctttaacct cgatttgatg catggactac cggatcaatc actggaagag
    85381 gcgcttggcg atctacgcca ggccattgaa ctgaatccgc cgcatctttc ctggtatcaa
    85441 ctgaccatcg aacctaatac gctgtttggt tcacgcccgc cggtactgcc ggacgacgac
    85501 gcgctatggg atatattcga acaggggcat cagttattaa ccgcagcggg ttatcagcaa
    85561 tatgaaactt ccgcttacgc caaatccagt tatcagtgcc agcacaatct caactactgg
    85621 cgctttggcg actacatcgg tattggctgc ggcgcgcacg gcaaagtgac cttcccggat
    85681 gggcgcattc tgcgtaccac taaaacgcgt catccgcgtg gttttatgca aggaaggtat
    85741 ctggaaagcc agcgtgatgt cgaagccgca gataagccgt ttgagttctt tatgaatcgc
    85801 ttccgtctgc tggaggccgc gccgcgcgtg gagtttattg cgtataccgg gctttgcgaa
    85861 gatgtgattc gcccacagtt agacgaggcg attgcccagg gttatctcac cgaatgtgcg
    85921 gattactggc agataacgga acatggtaaa ttattcttaa actcacttc
//
LOCUS       DAZGLJ010000002        71975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000002 DAZGLJ010000000
VERSION     DAZGLJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 71975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.002"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(225..1229)
                     /gene="trpS"
                     /locus_tag="ACHK49_000092"
     CDS             complement(225..1229)
                     /gene="trpS"
                     /locus_tag="ACHK49_000092"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO9260798.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(1222..1980)
                     /gene="gph"
                     /locus_tag="ACHK49_000093"
     CDS             complement(1222..1980)
                     /gene="gph"
                     /locus_tag="ACHK49_000093"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO9260799.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(1973..2650)
                     /gene="rpe"
                     /locus_tag="ACHK49_000094"
     CDS             complement(1973..2650)
                     /gene="rpe"
                     /locus_tag="ACHK49_000094"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO9260800.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKLSHE"
     gene            complement(2668..3504)
                     /gene="dam"
                     /locus_tag="ACHK49_000095"
     CDS             complement(2668..3504)
                     /gene="dam"
                     /locus_tag="ACHK49_000095"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO9260801.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(3611..4897)
                     /gene="damX"
                     /locus_tag="ACHK49_000096"
     CDS             complement(3611..4897)
                     /gene="damX"
                     /locus_tag="ACHK49_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO9260802.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(4989..6077)
                     /gene="aroB"
                     /locus_tag="ACHK49_000097"
     CDS             complement(4989..6077)
                     /gene="aroB"
                     /locus_tag="ACHK49_000097"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO9260803.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADSQSA"
     gene            complement(6134..6655)
                     /gene="aroK"
                     /locus_tag="ACHK49_000098"
     CDS             complement(6134..6655)
                     /gene="aroK"
                     /locus_tag="ACHK49_000098"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO9260804.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(7056..8294)
                     /gene="hofQ"
                     /locus_tag="ACHK49_000099"
     CDS             complement(7056..8294)
                     /gene="hofQ"
                     /locus_tag="ACHK49_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO9260805.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(8206..8610)
                     /gene="hofP"
                     /locus_tag="ACHK49_000100"
     CDS             complement(8206..8610)
                     /gene="hofP"
                     /locus_tag="ACHK49_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO9260806.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(8600..9040)
                     /gene="hofO"
                     /locus_tag="ACHK49_000101"
     CDS             complement(8600..9040)
                     /gene="hofO"
                     /locus_tag="ACHK49_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO9260807.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(9024..9236)
                     /gene="hofN"
                     /locus_tag="ACHK49_000102"
                     /pseudo
     CDS             complement(9024..9236)
                     /gene="hofN"
                     /locus_tag="ACHK49_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
     gene            complement(9231..9770)
                     /gene="hofM"
                     /locus_tag="ACHK49_000103"
                     /pseudo
     CDS             complement(9231..9770)
                     /gene="hofM"
                     /locus_tag="ACHK49_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
     gene            9890..12442
                     /gene="mrcA"
                     /locus_tag="ACHK49_000104"
     CDS             9890..12442
                     /gene="mrcA"
                     /locus_tag="ACHK49_000104"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO9260808.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(12608..13168)
                     /gene="nudE"
                     /locus_tag="ACHK49_000105"
     CDS             complement(12608..13168)
                     /gene="nudE"
                     /locus_tag="ACHK49_000105"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO9260809.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            13488..15623
                     /gene="igaA"
                     /locus_tag="ACHK49_000106"
     CDS             13488..15623
                     /gene="igaA"
                     /locus_tag="ACHK49_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO9260810.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWVPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            15688..16356
                     /gene="yrfG"
                     /locus_tag="ACHK49_000107"
     CDS             15688..16356
                     /gene="yrfG"
                     /locus_tag="ACHK49_000107"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO9260811.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            16367..16768
                     /gene="hslR"
                     /locus_tag="ACHK49_000108"
     CDS             16367..16768
                     /gene="hslR"
                     /locus_tag="ACHK49_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO9260812.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            16793..17671
                     /gene="hslO"
                     /locus_tag="ACHK49_000109"
     CDS             16793..17671
                     /gene="hslO"
                     /locus_tag="ACHK49_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO9260813.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILTEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(17806..19530)
                     /gene="yhgE"
                     /locus_tag="ACHK49_000110"
     CDS             complement(17806..19530)
                     /gene="yhgE"
                     /locus_tag="ACHK49_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO9260814.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYKSVWHNVLILLVIFLANGLTWVVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            19909..21531
                     /gene="pckA"
                     /locus_tag="ACHK49_000111"
     CDS             19909..21531
                     /gene="pckA"
                     /locus_tag="ACHK49_000111"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO9260815.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKELGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(21607..22959)
                     /gene="envZ"
                     /locus_tag="ACHK49_000112"
     CDS             complement(21607..22959)
                     /gene="envZ"
                     /locus_tag="ACHK49_000112"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO9260816.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIDVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(22956..23675)
                     /gene="ompR"
                     /locus_tag="ACHK49_000113"
     CDS             complement(22956..23675)
                     /gene="ompR"
                     /locus_tag="ACHK49_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO9260817.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            23903..24379
                     /gene="greB"
                     /locus_tag="ACHK49_000114"
     CDS             23903..24379
                     /gene="greB"
                     /locus_tag="ACHK49_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO9260818.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            24476..26797
                     /gene="yhgF"
                     /locus_tag="ACHK49_000115"
     CDS             24476..26797
                     /gene="yhgF"
                     /locus_tag="ACHK49_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO9260819.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVMSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQTAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            27235..27462
                     /gene="feoA"
                     /locus_tag="ACHK49_000116"
     CDS             27235..27462
                     /gene="feoA"
                     /locus_tag="ACHK49_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO9260820.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            27479..29800
                     /gene="feoB"
                     /locus_tag="ACHK49_000117"
     CDS             27479..29800
                     /gene="feoB"
                     /locus_tag="ACHK49_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO9260821.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            29800..30036
                     /gene="feoC"
                     /locus_tag="ACHK49_000118"
     CDS             29800..30036
                     /gene="feoC"
                     /locus_tag="ACHK49_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO9260822.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            30239..31117
                     /gene="rpnA"
                     /locus_tag="ACHK49_000119"
     CDS             30239..31117
                     /gene="rpnA"
                     /locus_tag="ACHK49_000119"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO9260823.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(31146..31916)
                     /gene="bioH"
                     /locus_tag="ACHK49_000120"
     CDS             complement(31146..31916)
                     /gene="bioH"
                     /locus_tag="ACHK49_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO9260824.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIVLTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            31954..32637
                     /gene="gntX"
                     /locus_tag="ACHK49_000121"
     CDS             31954..32637
                     /gene="gntX"
                     /locus_tag="ACHK49_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO9260825.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            32696..33271
                     /gene="nfuA"
                     /locus_tag="ACHK49_000122"
     CDS             32696..33271
                     /gene="nfuA"
                     /locus_tag="ACHK49_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO9260826.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            33632..34948
                     /gene="gntT"
                     /locus_tag="ACHK49_000123"
     CDS             33632..34948
                     /gene="gntT"
                     /locus_tag="ACHK49_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO9260827.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(34993..37077)
                     /gene="malQ"
                     /locus_tag="ACHK49_000124"
     CDS             complement(34993..37077)
                     /gene="malQ"
                     /locus_tag="ACHK49_000124"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO9260828.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(37087..39480)
                     /gene="malP"
                     /locus_tag="ACHK49_000125"
     CDS             complement(37087..39480)
                     /gene="malP"
                     /locus_tag="ACHK49_000125"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO9260829.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKMIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            40092..42797
                     /gene="malT"
                     /locus_tag="ACHK49_000126"
     CDS             40092..42797
                     /gene="malT"
                     /locus_tag="ACHK49_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO9260830.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(42840..43856)
                     /gene="rtcA"
                     /locus_tag="ACHK49_000127"
     CDS             complement(42840..43856)
                     /gene="rtcA"
                     /locus_tag="ACHK49_000127"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO9260831.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(43860..45086)
                     /gene="rtcB"
                     /locus_tag="ACHK49_000128"
     CDS             complement(43860..45086)
                     /gene="rtcB"
                     /locus_tag="ACHK49_000128"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO9260832.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            45275..46873
                     /gene="rtcR"
                     /locus_tag="ACHK49_000129"
     CDS             45275..46873
                     /gene="rtcR"
                     /locus_tag="ACHK49_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO9260833.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETQVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYTRINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(46855..47613)
                     /locus_tag="ACHK49_000130"
     CDS             complement(46855..47613)
                     /locus_tag="ACHK49_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO9260834.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(47630..48460)
                     /gene="glpG"
                     /locus_tag="ACHK49_000131"
     CDS             complement(47630..48460)
                     /gene="glpG"
                     /locus_tag="ACHK49_000131"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO9260835.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(48505..48831)
                     /gene="glpE"
                     /locus_tag="ACHK49_000132"
     CDS             complement(48505..48831)
                     /gene="glpE"
                     /locus_tag="ACHK49_000132"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO9260836.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            49021..50526
                     /gene="glpD"
                     /locus_tag="ACHK49_000133"
     CDS             49021..50526
                     /gene="glpD"
                     /locus_tag="ACHK49_000133"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO9260837.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(50578..51183)
                     /locus_tag="ACHK49_000134"
     CDS             complement(50578..51183)
                     /locus_tag="ACHK49_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9260838.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     AWGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(51186..51953)
                     /locus_tag="ACHK49_000135"
     CDS             complement(51186..51953)
                     /locus_tag="ACHK49_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9260839.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(51956..52666)
                     /locus_tag="ACHK49_000136"
     CDS             complement(51956..52666)
                     /locus_tag="ACHK49_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9260840.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANYKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCTRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(52682..54190)
                     /locus_tag="ACHK49_000137"
     CDS             complement(52682..54190)
                     /locus_tag="ACHK49_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO9260841.1"
                     /translation="MMQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVC
                     NILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLIL
                     VGWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSL
                     GYGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLE
                     SSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMITRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(54316..56763)
                     /gene="glgP"
                     /locus_tag="ACHK49_000138"
     CDS             complement(54316..56763)
                     /gene="glgP"
                     /locus_tag="ACHK49_000138"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO9260842.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(56782..58215)
                     /gene="glgA"
                     /locus_tag="ACHK49_000139"
     CDS             complement(56782..58215)
                     /gene="glgA"
                     /locus_tag="ACHK49_000139"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO9260843.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(58215..59510)
                     /gene="glgC"
                     /locus_tag="ACHK49_000140"
     CDS             complement(58215..59510)
                     /gene="glgC"
                     /locus_tag="ACHK49_000140"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO9260844.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(59528..61501)
                     /gene="glgX"
                     /locus_tag="ACHK49_000141"
     CDS             complement(59528..61501)
                     /gene="glgX"
                     /locus_tag="ACHK49_000141"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO9260845.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(61498..63684)
                     /gene="glgB"
                     /locus_tag="ACHK49_000142"
     CDS             complement(61498..63684)
                     /gene="glgB"
                     /locus_tag="ACHK49_000142"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO9260846.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(63957..65060)
                     /gene="asd"
                     /locus_tag="ACHK49_000143"
     CDS             complement(63957..65060)
                     /gene="asd"
                     /locus_tag="ACHK49_000143"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO9260847.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            65252..65845
                     /gene="yhgN"
                     /locus_tag="ACHK49_000144"
     CDS             65252..65845
                     /gene="yhgN"
                     /locus_tag="ACHK49_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO9260848.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(65902..67242)
                     /gene="gntU"
                     /locus_tag="ACHK49_000145"
     CDS             complement(65902..67242)
                     /gene="gntU"
                     /locus_tag="ACHK49_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO9260849.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(67246..67773)
                     /gene="gntK"
                     /locus_tag="ACHK49_000146"
     CDS             complement(67246..67773)
                     /gene="gntK"
                     /locus_tag="ACHK49_000146"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO9260850.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(67912..68907)
                     /gene="gntR"
                     /locus_tag="ACHK49_000147"
     CDS             complement(67912..68907)
                     /gene="gntR"
                     /locus_tag="ACHK49_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO9260851.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(69131..69826)
                     /gene="yhhW"
                     /locus_tag="ACHK49_000148"
     CDS             complement(69131..69826)
                     /gene="yhhW"
                     /locus_tag="ACHK49_000148"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO9260852.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(69949..70986)
                     /gene="yhhX"
                     /locus_tag="ACHK49_000149"
     CDS             complement(69949..70986)
                     /gene="yhhX"
                     /locus_tag="ACHK49_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO9260853.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADNSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            71319..71807
                     /gene="yhhY"
                     /locus_tag="ACHK49_000150"
     CDS             71319..71807
                     /gene="yhhY"
                     /locus_tag="ACHK49_000150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO9260854.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT        17415 a        19491 c        18095 g        16974 t
ORIGIN      
        1 aagataagtg gcaactgtga gatgtggaaa cggcgaggcg cttcacattt tttcggatta
       61 ttgcaaatga ggataaaaaa accgggtttc cccggtttca gagtgatgac gaagtgattt
      121 attcgacaat gcgagcaaag ggtaattccc actggcgctt gcctgctccc ctcaccctaa
      181 ccctctcccc aaaggggcga ggggaccgtc cgagcgtttt gatattacgg cttcgccaca
      241 aaaccaatcg cttcgtacac cgctttcagc gtacgggaag cgtgtacact ggctttttct
      301 gcgccgtctt tcatcacctg ttgcaggaag gcttcatcgt tgcggaaacg gtgatagcgt
      361 tcctgcaatt cagtcagcat accggaaacg gcatcagcca cttcaccttt cagatgacca
      421 tacatcttgc cttcgaactg tttttccagt tctggaatgc tctggcccgt taccgcagaa
      481 aggatatcca gcaggttgga aacgcccgct ttgttctgca catcgtagcg aactaccggc
      541 ggctcgtcgg agtcagtgac cgcgcgtttg attttcttca ctaccgattt cggatcttcc
      601 agcaggccga taacgttatt gcggttatcg tcagacttgg acatcttctt ggtcggctcc
      661 agcagcgaca ttacgcgcgc gccagatttc ggaataaacg gctccggcac cttaaagatc
      721 tcgccataca gcgcgttgaa acgctgggcg atatcgcgac tcagttccag gtgctgtttc
      781 tggtcttcac ccaccggtac cagattagtt tgatacagca ggatgtccgc agccatcagc
      841 accggatagt caaacagacc agcgttgatg ttctcggcat aacgcgcaga tttatcttta
      901 aactgcgtca tgcggctcag ttcgccgaag taggtatagc agttcagtgc ccagcctaac
      961 tgcgcatgtt ctggtacgtg ggactgaaca aaaatggtgc ttttctcagg atcgatacca
     1021 caagccagat acaaggccag cgtatccaac gtcgctttac gcagcttctg tgcatcctgg
     1081 cgcacggtga tcgcgtgttg gtcaacgata cagtaaatgc aatggtagtc atcctgcatg
     1141 tttacccact gacgcagcgc acccatgtag ttaccaatgg tcaattcacc tgagggctgt
     1201 gcgccactaa aaacgatggg cttagtcatt tttcgattcc tgattttcgc tatgcggaag
     1261 ccctaatgcg ggcagaaggt catttataga ctgataaatt acatcaggct ggctgagatc
     1321 gatagcctcg ccgtagttat atccgtaggt taagccaact gatgggcaac ctgccgcttt
     1381 tgccgcctga atatcattgc gtgagtcgcc gacaaacagc atctgttgtg gggcaattcc
     1441 catccgctca gccaccagta acagcgggtc cggatgcggt tttttgtttt gcacatcatc
     1501 gccgccgata accacgctaa agtatttggc aatatctaag gcttcgagca gcggcgcgac
     1561 gaacggcgtc ggtttgttgg tgaccaggcc tagcggcagg cctttagcct gcaacgcgcc
     1621 caacgtatcg gcaacgtgcg ggaacaaaaa cgtcccctct tcggcaacct cgccatagta
     1681 gcgatcgaac agtttacgca gaatacgtac ctgttcttct gccggaatgt cgtcatcaac
     1741 gggcggttta cccattgttt tacgctgagt cgcacgttcc tgacgcgccc aggtcaatgc
     1801 gcgctccatc agaacatctg cgccgttacc aatccaggta ataacgcgtt cttcacctgc
     1861 gacgggcaac tccagcgcat acagcgccat atctaccgca gcagcaagac cgggagcact
     1921 gtcgaccagc gtaccatcaa gatcaaaagc gacgccgcga atatcttcaa acttattcat
     1981 gacttaactt tgccagttca ctgcgcattt catcaatgac ttttttgtag tctggctggt
     2041 cgaagattgc cgaaccggcg acgaacatat ccgcgcccgc cgcagcgatt tcgccaatgt
     2101 tgttcacctt cacgccaccg tctacttcca gacgaatgtc aaagccagac tcgtcgatac
     2161 gacgacgtac ttcgcgcagt ttatccagtg tttgaggaat gaacgactga ccgccgaaac
     2221 caggattgac ggacatcagc aggatcacat ccagcttatc catcacgtaa tccagataac
     2281 tcagcggtgt cgccgggtta aataccagtc ccgctttaca gccattttct ttaatcagtt
     2341 gcagcgtgcg gtcaacatgc tcggaggctt ctggatgaaa ggtaatgatg ctggcaccag
     2401 cggcagcgaa atcaggcaca atgcgatcga caggtttcac catcaggtgt acgtcgatag
     2461 gggcggtaat gccgtagtta cgcaaggatt tcagcaccat tggcccaatc gtcagattgg
     2521 gaacatagtg gttatccatg acgtcaaaat gcacgacatc agcgccagct gccagggctt
     2581 ttgcggtatc ttcacccagg cgggcaaaat cagccgacag aattgagggg gcaatcaaat
     2641 actgtttcat ccgcttctcc ttgagaatta ttttttcgcg ggtgaaacga ctcctggttt
     2701 gtacaaagcc agcagttcgt ccaccttttt acgtgtgccg ccgttgctgc ttatactgcg
     2761 tcgaactttg acgacatgca attttgcgcg ctgataccac tcacgcgtta acatcgtatc
     2821 gtgattggag atcagcactg gaatatggcg ctcaaccaga ccttcggcga tctccgccag
     2881 atgcgcttgt tgttcaagcg taaaactgtt tgtgtgatac gccgtaaagt tggcggtcgc
     2941 agacagcggt gcataaggcg gatcgcaata gacgacggat gcatcatctg cgcgcgccat
     3001 gctatcggcg taagactcac aatagaaaaa ggcattctgc gctttttcag cgaagtgata
     3061 caactctgct tccgggaaat agggtttttt gtagcggcca aacggcacgt taaactcacc
     3121 gcgcagattg taacgacaca ggccgttgta accgtagcgg ttcaaatata aaaacagtac
     3181 cgcccgacgg aacggatcct ggcttttgtt gaactcttcg cggaactgat agtagacctc
     3241 ggcgcaattt gtttcgggaa caaacagctc gcgtgcggcc tgtacgtact catcagtacg
     3301 catcttcaca atgttataga gactgatcag gtcgctattg atatcggcaa ggatataacg
     3361 agaaaagtcg gtgttgagaa acaccgaccc ggcacctaca aaaggctcaa ccagacattc
     3421 gcccttgggc aaatgccgtt taatatcatc aagcagggga tacttgcccc ctgcccactt
     3481 caaaaaagcg cgatttttct tcatgctgac taactaatta caccttctcc ggctgtggag
     3541 aaagctccga cagcatccag cgcttcaggc aactcccgca acgataaccc attgcgggag
     3601 ataaccttga ttacttcaga tcggcctgta cctgacgcag cggtttcgcc cacgggtttt
     3661 tggcctgaac atctgctggc aatgtagata ccgctttttt cgcctcttct ttcgaagcgt
     3721 acacgccaga aaccaggaca taccacggct gaccattacg cgtcgtttca tagacaacgt
     3781 agtttttcag attctctttc ttcgcccaac cgttcaggtt gtcgtagtta gaggaactgc
     3841 tcagctgcag agtgtaatgg ctggacggtg ccgatttcaa cgaaccaaca ttacctgcgg
     3901 tcttccctcc agcggctggt gttgccgtgg tttgcgccgg ggatgccgtc tgtactggag
     3961 ccgtagtcgc cgtctctttt ggtgctggcg cagaagtcgc agccggtgcc ttcgtgctcg
     4021 ccacaggagc agctggttca gtacgcttcg gcgtctgtgc taccggcttc ggttccgttt
     4081 tcacggttgc ttgcggtttt ttcggttcaa tcaccgcctg ctgacgagct ggacgcgtag
     4141 tggccggacg ttcagcagtt tgcgttttcg ccgtgtcacg cgatgcattg ccattgcgta
     4201 ctggcgcgac agttgctggt tcagtcggca atgtagaatt gaccgccaca ttgttcaact
     4261 gttgctgatt ttgtggctgg gtcagcgcat tgttcaggtc accctgcact tcaacacgtt
     4321 gttgaccatc cgttgccgcc ggggtttgcc cttgagtcgg cgtagaagag atcggtggca
     4381 gagaaacatc ctgctgagta ttttccgcag acgtggttcc cggcgctggc tgcaccccat
     4441 tcgcctgatc ggtcgcattg cctgcaagat caatactctt ctcgccagac gcggtttgat
     4501 cgctggaaga ggtcgagggg gcttttagcg cagaaccgat accgatgatc aacagcagta
     4561 gaaccagaat gccgacgccc atcatcatat actgacgaga agcgggttta ctggctgctt
     4621 ttttgcgctt acgcggacga cgctctacgc gctcttcatc cacggtttca tcttcggatt
     4681 cgtcaatttc ttcttcgatt tccggttcct cattgcgctc tttttgcgca cgagtcggac
     4741 ggcgatcgtc agtgtcatca agttcaatat catcaaaatt gatctgcggt tcgccacgtt
     4801 cagtacgctc agaacgttca gaagattgac gagaacgacc agtacgacga tcgctgggat
     4861 cgggtttcag ctcgtcttct ggtttgaatt catccattta acaccccact aaaaggttaa
     4921 tgcttaccac attgcaatta acctgaagct aatagaccgc ttgataagcg gcctgacctt
     4981 tcttgttgtt acgctgattg actatcggca atggcgttaa gaacaagctc gtgcgaaacg
     5041 ccgctgcgaa cttcactctt accaattgcc aacggaagaa ttaagcgtat ctctcccgca
     5101 aggactttct tgtcacgcag catatgcggt aaatacgcct gcgcggacat ttcgcgcggc
     5161 ccattgaccg gtaacccagc ccgcttgagc agggttataa tacgctgcgt ttcggcagaa
     5221 ctaaactgcc cgagacgttc cgacgtccgc gccgccatca ccatacccgc agcgaccgct
     5281 tcaccatgta accaattgcc ataccccatt tcagcttcaa tggcatgacc aaaggtgtgt
     5341 cccagattca gtaaagcacg taacccggtt tcgcgctcgt cggcggcgac aacttctgcc
     5401 ttcagttcac aacaacggcg aatacagtac gccattgccg gaccgtccag acgcaacaac
     5461 gcatccagat tctcttccag ccagttaaaa aacgcaccgt caagaataat gccgtatttg
     5521 atgacttctg ccagccccga cgctaactca cgcgggggaa gcgttttcag acagtcgaga
     5581 tccaccacca ctgaagcagg ctggtagaac gcgccaatca tgtttttacc gaggggatgg
     5641 ttgaccgcag ttttgccgcc aacggaggaa tcgacctgcg acagtaacgt cgtcgggact
     5701 tgaatgaaac gaacaccgcg ctgataactc gccgccgcga agccggtcag atcgcccact
     5761 acgccgccgc caagcgccac cagcgtagta tcgcgaccat gcggcttttg taacaacgcc
     5821 gtaaagacgg tatcgagtac agccaggctt ttatactgct cgccgtcagg gaggataacg
     5881 ctatcgacgt taacacccgc ctgttcaagt acgccgcgga ccttatcgag atacagagga
     5941 gccagggttt cgttggtgac caacatgacc tgctcgcccg atttcagcgg taagaatgaa
     6001 gctggttcat taaacaaacc agatgcgatg gtaattgggt aactacgttc cccgagagta
     6061 acgacaatcc tctccataac gcgacatcca ccttaattac tgtacccgca gacgagtgta
     6121 tataaagcca gaattagttg ctttccagca tgtgaataat ctggtttgca accactttag
     6181 cgctttgatc atcagtacga atggtcacgt cggcaatctc ttcatacagc ggattgcgtt
     6241 cattggccaa cgcttccaga acttcacgcg gcggtgtttc aacgtgcagc aacgggcgtt
     6301 ttttatcacg ctgcgtgcgt gcaagttgct tttcgatggt cgtttcaaga taaacgacaa
     6361 cgccacgagc ggaaagacgg ttacgcgttt cacgggattt cacagagccg ccgccagtag
     6421 ccagcacaat accctgtttc tcggtcaact cattgatgac cttttcttcg cgatcgcgga
     6481 agccttcttc gccttctaaa tcgaaaaccc agcccacatc agctccggtt cgtttctcaa
     6541 tctcttgatc ggaatcgtaa aattccatat tgagttgttg agctaactgg cgcccaatag
     6601 tgctttttcc ggcacccata ggcccaacca gaaagatatt gcgtttctct gccatttttt
     6661 cggtactact aagactattc gttaatgata aacccgcttc gctcagagag cgccgcagga
     6721 catgaactga aacctcataa gatattgcga gagtcagact gaaaattatc tcaatactcc
     6781 agcgggtttg gcaactgaat aaatcaccca tccctctgca tatctggtcg ctgcaagcgc
     6841 gctgccttgc taccaccgct ctggcgataa atcaccgggt aagattagcg taaaaaagac
     6901 agcaaaatgc cgcctgaatg ataaatcatc atcatggaac gcaggcagcg aagagcacca
     6961 actctcaaat cggtactcct tgtatgctaa atacctgcgc gcgtcaaata gatgaaacac
     7021 gttcagcata aaaacattac cgcttttacg gctgtttact cactggaaac cagtcgtggc
     7081 gtgataaaca ccactaactc gcgtcgttca tcttcttttc cgtcatgacg aaataattgc
     7141 ccgaaccagg gaatgtcgcc aagcaacggt acgctatcct gacccgattt atttttacgg
     7201 gtaaaaatgc cgcccagcgc caacgtttct ccgcttttga cctcgacctg cgtttcgatc
     7261 tcctgcttat caatcgccag cacttcgcca tcggcctgct gtagcacctg ccccggaacg
     7321 ttctggctga tgtgtaattt cagccggatg cgaccttttt gtaacaccgt gggcgtgacc
     7381 tccatcccca ggacggcctc tttaaattcc accgacgtcg cgccactttc cccgctggaa
     7441 acctgatatg gaatttcgct cccctgttta atgctggcag gctgaagatg tgaggccagc
     7501 agacgcggac tggcgataat atccagctgc tgtttttgtt cgagcgcaga aagctcaaga
     7561 tccagcaagc gtccgttgat gcgcccaatg ttaaaaccga catgcgttgt cgccgtcgct
     7621 acggagaggt cgctaccaag cgtggtgact tgcccaacgc caccagcgtg ttgcgcatcg
     7681 gccagcgtcc atttcacgcc taactcacgc aaactttttt cattaatggt gacaatatgc
     7741 gccgacagct caacctgccc gaccggcaga tccatttgcg ctacccactg ttcaagcgcg
     7801 cttaacgccg ttttgttatc tcgtagcaaa aggcgattgg tgcgtttatc gacggtcata
     7861 ctccctttgg cactcagtag cttctccccc gctttcgcca gttctcccgc gtcggcgtat
     7921 tgcagggtta tactgcgatt ttccagcggc agatttgcct gcgcccgcgc ctgctccgcc
     7981 tcctggcggg cgatattgtt attctgccag gcaatggaat gcactgagag aatgttgtct
     8041 tcctgccgcg ttatcagtcc ggcgcttttc actacagttt gtagtgcctg cttccaggga
     8101 acatctgtta gatgtaacga caccgtaccg ctgacgtctg gcgacacgac caggttcaac
     8161 ttctcctgtt cagccagcgc ctgcaacacc tgagctaccg gaacgtcatc caccatcagc
     8221 gtcacttttt gcggctttgc cgcctgtacg ccgggtatca gcatcaacag tagtgcggct
     8281 atccattgct tcatttgtat ctccttgccg ttgccacaac cattgtggcg gttcgcagtt
     8341 tgtcccggtt cccagcgtta gtgcgtctgg cgtcagctgt aaaattgtcc agccgttttc
     8401 cagcacatcg ttttgctgca cccgtcgcca tttcttttgc ccgtctttta ttacaccgat
     8461 gatgtgctcg cctcgcccta ccatcccctg atagcgccac tggctaagtt cgctaatccg
     8521 gcatagatct tccggcggtt taaaagggtc acgcataccg gttaaaaggc acaatgcaat
     8581 acctgccaac aaccagcgtt taaccctcat gcggtgtctc cagttgtagc gtgaacaaaa
     8641 gatcatcacc ttccacgctt aacgaaaaac ggctcacgct gacgttgcgc tctgccagcc
     8701 gtgtaaatgc cgacggcact gcttcccaca gcgttttcaa cgccaactca cctccctgcg
     8761 cggatggatg ccaggaaacc agttgcgcgc cgggcaactg aaaatccagt ggcgaaaagg
     8821 gcagcgtttt ttcttcgctg aagggagtgg tgtcaaccag gcgatacagt gtggcccact
     8881 gctggtgatg acttgcccgt agtcgaatta atgcgtcgcg ctcttcatgg tgtgtcgatg
     8941 acagaaaaat gagcgtaact aacatcagca accagaatgc ccagcaaaac tggcggaggc
     9001 ggggtgatgt ggcgaaccac cagtcaaaga acatgttcat cgctaacctt ccttgttaac
     9061 tgatactcaa attgccagcg tccctgcgca tcctgctgcg tggctccccg ctgattgaga
     9121 tgaaaagacg catcctggcg gagtgacgtt tctagtgcgt ttaacgcggt aatgcttgtt
     9181 gtcagcccct tgatctccag cgttccctgc tgccagctta tcgttgtcag ccaggccaga
     9241 cattgctgat gagaaggtaa aaaaggcagg aatcgctgta atgcactggc atccggggtg
     9301 atcgcactaa catgaacacg caacctttct gccagagtaa gcagcgttgc cagctctttg
     9361 ctttgcgcgg cagtcacgtt ataggcggga ctgagtgagt cttcgctgta atcgaaacgc
     9421 agggagtccg gatccatatc cagctcgcgg gccatcgtcc ctgacagcca ggccgtttgc
     9481 tcccgctcac caagggacat cgacgggcgc ggaaatgacc gctgtaatgt gcgactggcg
     9541 ggaaacgcca acataatgtg atgacgctgc ggcagttcgc gactccacgg taacaacgtt
     9601 ttagccagct gctgcgcatc aacaatccgc ccatctttga taatgtcgtt ctccagcggc
     9661 aaccgccacc agcgttgcaa aaagcattct tttgctcccc gcacgatcgc aaccgctacc
     9721 gcttcttgct gttgtaaatg caaaccaatt tgccagatct taaatgccat tgtgatgctc
     9781 tccttatcac ccgtcactct gacgggtata tcaatgcgtc tggcttgcct ttatactacc
     9841 gcgcgtttgt ttataaactg cccaaatgaa actaaatggg aaatttccag tgaagttcgt
     9901 aaagtatttt ttgatccttg cagtctgttg cattctgctg ggagcaggct cgatttatgg
     9961 cctataccgc tacatcgagc cacaactgcc ggatgtggcg acattaaaag atgttcgcct
    10021 gcaaattccg atgcagattt acagcgccga tggcgagctg attgctcaat acggtgagaa
    10081 acgtcgtatt ccggttacgt tggatcaaat cccaccggag atggtgaaag cctttatcgc
    10141 gacggaagac agccgcttct acgagcatca cggcgttgac ccggtgggga tcttccgtgc
    10201 ggcaagtgtg gcgctgttct ccggccacgc gtcacaaggg gcgagtacca ttacccagca
    10261 gctggcgaga aatttcttcc tcagtccaga acgcacgctg atgcgtaaga ttaaggaagt
    10321 cttcctcgcg attcgcattg aacagttgct gacgaaagac gagatcctcg agctttatct
    10381 gaacaagatt tacctcggtt accgcgccta tggtgtcggt gctgcggcac aagtctattt
    10441 cggtaaaacg gtcgaccaac tgacgctgaa cgaaatggct gtgatcgccg ggctgccgaa
    10501 agcgccttcc acctttaatc cgctctactc aatggatcgt gccgtcgcgc ggcgtaacgt
    10561 cgtactgtcg cggatgctgg atgaagggta tatcacccaa caacagttcg atcagacacg
    10621 cactgaggcg attaacgcta actatcacgc gccggagatt gctttctctg cgccgtacct
    10681 gagcgaaatg gtgcgccagg agatgtataa ccgttatggc gaaagtgcct atgaagacgg
    10741 ttatcgcatt tacaccacca tcacccgcaa agtgcagcag gccgcacagc aggcggtacg
    10801 taataacgtg ctggactacg acatgcgcca cggctatcgc ggcccggcaa atgtgctgtg
    10861 gaaagtgggc gagtcggcgt gggataacaa caagattacc gatacgctga aggcgctgcc
    10921 aacctatggt ccgctgctgc ctgccgcagt caccagcgcc aatcctcagc aagcgacggc
    10981 gatgctggcg gacgggtcga ccgtcgcatt gagtatggaa ggcgttcgct gggcgcgtcc
    11041 ttaccgttcg gatactcagc aaggaccgac gccgcgtaaa gtgaccgatg ttctgcaaac
    11101 gggtcagcaa atctgggttc gtcaggttgg cgatgcatgg tggctggcac aagtgccgga
    11161 agtgaactcg gcgctggtgt cgatcaatcc gcaaaacggt gccgttatgg cgctggtcgg
    11221 tggctttgat ttcaatcaga gcaagtttaa ccgcgccacc caggcactgc gtcaggtggg
    11281 ttccaacatc aaaccgttcc tctacaccgc ggcgatggat aaaggtctga cgctggcaag
    11341 tatgttgaac gatgtgccaa tttctcgctg ggatgcaggt gccggttctg actggcagcc
    11401 gaagaactca ccaccgcagt atgctggtcc aattcgctta cgtcaggggc tgggtcagtc
    11461 gaaaaacgtg gtgatggtac gcgcaatgcg ggcgatgggc gtcgactacg ctgcagaata
    11521 tctgcaacgc ttcggcttcc cggcgcaaaa cattgtccac accgaatcgc tggcgctggg
    11581 ttcagcgtcc ttcaccccaa tgcaggtggc gcgcggctac gcggtcatgg cgaacggtgg
    11641 cttcctggtg gacccgtggt ttatcagcaa aattgaaaac gatcagggcg gcgtgatttt
    11701 cgaagcgaaa ccgaaagtag cctgcccgga atgcgatatt ccggtgattt acggtgatac
    11761 gcagaaatcg aacgtgctgg aaaataacga tgttgaagat gtcgctatct cccgcgagca
    11821 gcagaatgtt tctgtaccaa tgccgcagct ggagcaggca aatcaggcgt tagtggcgaa
    11881 gactggcgcg caggagtacg caccgcacgt catcaacact ccgctggcat tcctgattaa
    11941 gagtgctttg aacaccaata tctttggtga accaggctgg cagggtaccg gctggcgtgc
    12001 aggtcgtgat ttgcagcgtc gcgatatcgg cgggaaaacc ggtaccacta acagttcgaa
    12061 agatgcgtgg ttctcgggtt acggtccggg cgttgtgacc tcggtctgga ttggctttga
    12121 tgatcaccgt cgtaatctcg gtcatacaac ggcttccgga gcgattaaag atcagatctc
    12181 aggttacgaa ggcggtgcca agagtgccca gcctgcatgg gacgcttata tgaaagccgt
    12241 tcttgaaggt gtgccggagc agccgctgac gccgcctccg ggtattgtga cggtgaatat
    12301 cgatcgcagc accgggcagt tagctaatgg tggcaacagc cgcgaagagt atttcatcga
    12361 aggtacgcag ccgacacaac aggcggtgca cgaggtggga acgaccatta tcgataatgg
    12421 tgaggcacag gaattgttct gattaaaaag gcgcttcggc gccttttcag ttggctgaca
    12481 aagtgcattt gtttatgccg gatgcgggtg aacgcgttat ccggctaaca aaatcgtgaa
    12541 aaaatcaata tattgcagga aacccataag cctgataagc gttgtgcatc aggcaaactt
    12601 cacgcattta cactcgcccc tgccctttca accattcgcg cacgaggaac agcgcgctga
    12661 cattacgcgc ttcgttgaag tcagggtctt ccagcaaatc catcatatgc gccagcggcc
    12721 agcgcacctg tggtagcggc tctggctcat cgccttccag cgattccggg tagagatctt
    12781 gcgcgaccac gatattcatt ttgctggaaa agtaagacgg tgccatgctg agcttcttca
    12841 aaaaagtcag atcgttcgca ccaaatccaa cctcttcttt tagctcgcgg ttagcggctt
    12901 cgtagacgct ttcacccgga tcaattaatc ctttcgaaaa acctaattcg taggattcag
    12961 ttcccactgc gtattcgcgg atcaggatca ggtgatcgtc cacaatcggc acaatcatca
    13021 ctgcttcccg gttggttgga cgcattcgtt cataaacacg ccgcacgcca ttgctgaact
    13081 ccagatccac gctctcgacg gtaaacagtc gggaacgggc tacagtttca acattcagaa
    13141 tggtgggttt ttgtaatgat ttgctcatcg tgggatctat gctgtgaaat cagcggttat
    13201 tgtgcgatat cggacacgct ttcggcaatg tgaattgcat gttatttaca tttatgtaac
    13261 ttaataaata atcgtcctca aatcaaatta aaagtcaata ggttgaaata actccaggaa
    13321 tttgctgata ttccgccttc agagggtttg ctatgatcag cggttactgt gatgtgctta
    13381 atgatgctca agttaaactc cacgcttgcc gatagccaac cgcagaatca tgtattgtgt
    13441 ctggtgcgac tgaccacgcc tgacagacta agtaagatgg ggaaagcatg agcaccattg
    13501 tgattttttt agctgctttg ctggcctgct cactacttgc gggatggctg ataaaagtgc
    13561 gatccagacg gcgtcagctg ccctggacca acgccttcgc ggatgcgcaa acgcgtaaac
    13621 tcacacctga agaacgtagc gccgttgaaa attatcttga gagcctgacg caggtattac
    13681 aggtgcctgg cccaacggga gccagcgcgg caccgatctc tctggcgctg aatgccgaaa
    13741 gcaacaacgt catgatgctg acacacgcta tcacgcgtta cggcatctct accgacgatc
    13801 cgaataaatg gcgttactac ctcgattcgg tagaagtcca cctgccccct ttctgggaac
    13861 aatacatcaa cgatgagaat accgttgaac tgatttatac cgattcgctg ccgctggtta
    13921 tttcactcaa cggtcatacg ctgcaggagt acatgcagga aacgcgcggc tatgccttgc
    13981 aacctgttcc gtccacgcag gcgtcgattc gcggggaaga gagtgagcaa atcgagctac
    14041 tcaatattcg caaagaaacg catgaagaat atgcgctgag tcgtccgcgc gggctgcgtg
    14101 aagcgttgct gatcgtcgcc tccttcctga tgttcttttt ctgcctgatt accccggatg
    14161 tatttgttcc gtggctggca ggcggcgcat tactgctgct gggcgcaggt ctgtgggggc
    14221 tattcgcgcc cccggcaaaa tcctccctgc gagaaattca ttgtctgcgc ggtacacccc
    14281 gtcgttgggg attgtttggc gaaaacgatc aggaacagat caacaatatt tcactcggta
    14341 ttatcgacct ggtctatccc gcacactggc agccatacat tgctcaggat ctcggtcaac
    14401 aaaccgatat tgatatctat ctcgaccgcc acgtagtgcg tcaggggcga tatctttcgc
    14461 tgcatgatga agtaaaaaac tttccgttac agcactggct gcgcagtacg attatcgctg
    14521 cgggctcgct gctggtgctg tttatgctgt tattctgggt cccgctggat atgccgctga
    14581 aattcactct ctcatggatg aaaggcgcgc agaccattga agccaccagc gtaaaacaac
    14641 tggctgacgc tggcgtgcgg gtaggcgata cattgcgtat tagcggtacg ggaatgtgta
    14701 atattcgcac ttccggaacc tggagcgcga aaaccaattc acctttttta ccgtttgact
    14761 gctcgcagat catctggaat gacgcccgct cattaccatt accagaatct gaactggtca
    14821 acaaagcgac ggcattgact gaagcagtta atcgccagct gcaccctaaa ccggaagatg
    14881 aatctcgcgt cagtgcctca ttacgttcag cgattcaaaa atccggcatg gtattgcttg
    14941 atgattttgg cgacattgta ctgaagacag cggatttatg ttctgccaaa gatgactgtg
    15001 tgcgactgaa aaatgcgctg gtcaatctcg gcaacagtaa agactgggac gcgctggtaa
    15061 aacgcgccaa cgccgggaag ctcgatggcg tgaatgtgtt attacgcccg gtgagtgcag
    15121 aatcgctgga taacctggtg gcaacctcca ccgcgccgtt tatcacgcat gaaacggcgc
    15181 gagcggcaca atcactaaac agtccggccc ccggcggatt cctgattgtc agcgacgaag
    15241 gcagcgattt tgttgatcag ccctggcctt cggcatcact ttacgactac ccgccgcaag
    15301 aacagtggaa cgctttccag aaactggcac aaatgctgat gcatacgccg tttaacgccg
    15361 aaggtatcgt cacaaaaatc ttcactgacg ccaatggtac gcagcatatt ggccttcatc
    15421 cgatcccgga tcgttccgga ctgtggcgct atctcagcac cacattgctg ctactgacga
    15481 tgctgggtag cgccatttac aatggcgtac aggcctggcg tcgttaccag cgtcatcgca
    15541 ctcgcatgat ggagattcag gcctattatg aaagctgcct gaacccgcaa ctgatcaccc
    15601 cttcagaaag ccttatcgaa taacacgttt gcgcggcagg ttatgctacc ctgtcgcgca
    15661 aattgcttca ctctggagat ttccctcatg catatcaaca ttgcctggca ggacgtagat
    15721 accgttctgc tggatatgga cggcacgttg ctcgacctcg ccttcgataa ctatttctgg
    15781 caaaagctgg tgcctgaaac atggggcgcc aaaaacgggg ttacgccaca ggaagcgatg
    15841 gaatatatgc gccagcaata tcacgacgta cagcatacgc taaactggta ctgtcttgat
    15901 tactggagtg agcaactggg tctggatatc tgtgcgatga ccaccgagat gggaccgcgt
    15961 gccgtactgc gtgaagatac cattccgttt cttgaggcac tgaaagccag cggtaagcag
    16021 cgaattttgc tcaccaatgc gcatccgcac aacctggcgg taaaacttga gcataccggt
    16081 ctggacgcac accttgattt attactttcc acccacacat ttggttatcc gaaagaggat
    16141 cagcggttat ggcatgcggt ggccgaagct acgggtctga aagctgaaag aacgctgttt
    16201 attgatgaca gcgaagcgat tctcgatgct gccgcgcaat ttggtattcg ttactgcctc
    16261 ggcgtgacta atcctgattc cgggattgcc gagaaacagt atcaacgcca tccgtcactg
    16321 aatgactacc gccgcctgat cccctcgcta atgtgaagga gacgccatga aagagaaacc
    16381 tgctgttgag gttcgactgg ataaatggct atgggctgcc cgtttttata aaacccgcgc
    16441 gctggcccgt gaaatgattg aaggcggtaa ggtgcattac aacgggcagc gcagcaagcc
    16501 gagcaaaatc gtcgagctga atgccacgct cactctgcgc cagggaaatg acgaacgcac
    16561 ggtgattgta aaggcgatta ctgaacagcg tcgccccgcc agcgaggcag ccttgctgta
    16621 tgaagagact gcggaaagtg tagagaaacg cgaaaaaatg gcgatggcac gtaaacttaa
    16681 tgccttaacc atgccgcacc cggaccgacg cccggacaaa aaagagcgcc gcgacctgtt
    16741 acgatttaaa cacggcgaca gtgaataact gtcacctgca agagagatga ttatgccgca
    16801 acatgaccaa ttacatcgct atctgtttga aaattttgcc gttcgcggcg aactggtaac
    16861 cgtttcggaa accctgcaac agatccttga gaaccacgat tatccgcagc ccgttaaaaa
    16921 cgtgctggca gaactgctgg ttgcgaccag cctgttaacc gctacgctga agtttgatgg
    16981 tgatatcacc gtacagctgc aaggcgacgg tccgatgaat ctggcggtta ttaacggtaa
    17041 caataaccag caaatgcgcg gtgtggcgcg cgtgcagggc gaaattccag aaaatgccga
    17101 cctgaaaacg ctggtcggca atggttacgt ggtgatcacc attaccccga gcgaaggcga
    17161 acgctatcag ggcgtggttg gtctggaagg tgataccctg gcggcctgcc tggaagatta
    17221 ctttatgcgt tctgaacagc tgccgacgcg cctgtttatt cgcaccggcg acgtagacgg
    17281 caaaccggct gcaggcggta tgttgttgca ggtaatgcct gcgcaaaatg cccagcagga
    17341 cgactttgac cacctggcga cgctaaccga aaccatcaaa accgaagaac tgctgacctt
    17401 accggcaaac gaagtgttgt ggcgtttgta tcacgaagaa gaggtgacgg tttacgatcc
    17461 gcaggatgtg gagttcaaat gcacctgctc gcgtgaacgt tgcgccgatg cgctgaaaac
    17521 gctgcctgat gaagaagttg atagcatcct gacggaagat ggcgaaattg acatgcattg
    17581 tgattactgc ggtaaccact atctgttcaa tgcgatggat attgctgaaa tccgcaacaa
    17641 cgcgtctccg gcagatccgc aagttcatta atgagtatgt ccggcagaga gggctctgcc
    17701 ggattttacg acgtaatggc ctgatgtgct gcgcttacca ggcctgtgaa ataaatcgca
    17761 aattattgtt ggtcagatac agcgtcgtat ccgttaaaag agttgtcact tacttaagtt
    17821 tatttccagc gtttcaccat ctaccgtcag atcgcgccac gctttcggcc tcgtccccag
    17881 ttttccatcc ttcgcagctg tgagtaattt ttctttcgtt atttcgttcg gaagtaaact
    17941 catatcaagc gcgtcccatt cttttctgtc tgagtcaaag ccatagacaa tgactctgtc
    18001 atcattaaaa gcaaacagga tccgctccgc ctgaccatca ctattcagat cttgctcgac
    18061 caggacgcag gcgtcttttt caatacaggt catcacgtta tagcgatcct ggattaaggc
    18121 cgaccagaat gtcgcatccg gtttaacaga accaggggca attaacacat tttcggctaa
    18181 tactttttcc gatacctgtt cctgaagatg ttgctctcca tctaacgcca tcagcagatc
    18241 ccgcgcgcgt ttcgggtctt tcataaaccc ggcatcgctt ttcagcgact caagcgccgc
    18301 acgtccatag cgaccgctct gttcgagcat gtagatagtt acctggtctg gcgtgttttt
    18361 gccgctctga taacgcgcca tatgactgtt cacgctaata cgcatactat ccagcaccgg
    18421 cgaattaaga agcaccagta tcaccaacac caataacgaa accgcaaggt tcactttgcc
    18481 ctgaagaaca aggggatttt gcccattacg ccagacgata ctgacaaaat accccagcga
    18541 ccacaccagt aacaccagca ccgccagcgc gccctgcaag cggtcgacag tccagccgta
    18601 ctgagcgacc cgcagccata acgcccaggc ggcgacgaac acatacagcg gagcaaccag
    18661 caaagcggtt ttaatcaggc aacgcaacgg ccccgtccag ggaagtgacg ctttttgcgg
    18721 atcgcggaca atagccatta agatcaattg caaaaaggcc agcgttaaca gcaacccggc
    18781 ggcggagatg tggcgagaaa tcgcgctcag gcccgtaaac ggcagagtga tgataaacat
    18841 cagggttagc aatgatacta acggcagcaa ccccgtggcg attaacgtga acaacttttg
    18901 aatagagtcg attaaacgtg actgtgtgcg cgcgagaatc accgccagtg cggtaaccag
    18961 acctaatgta agatacataa accagtcggt tgcaaaaaag agcgtattga aaaacttgat
    19021 gccaacaagt ttaaacaact caccccacag taaaagcacc acccacgtca ggccattggc
    19081 gagaaaaatc accagtaata taagtacgtt atgccatact gacttgtaaa agtagctata
    19141 gcggctactg ccattgcgga tacgaaggct ttgttgtatc cacggtagca acaacattgc
    19201 catcaacagc agatagcagc caaaatccca aatcgccttt tcagctctcc aggggttcat
    19261 gccatcagtt tgccacttca gccaaccgct catccctgtc gtggcaataa acaccagcgc
    19321 cagccatccc caaaggcgct tctgtttaaa agagatcacg gagaagagta aaacggatga
    19381 aaacgcaacg gtcgcaggca cgccataaac aatccaactg tgattttttc cggcaagcca
    19441 ggcgatcaac aggtagcaaa ccagcccctg aagtaatccg gtagcaatca tcccccagcg
    19501 tgttgccggt gaaagttcaa cgttatccat gttggttatc cagaatcaaa aggtgggtta
    19561 attatcgcat ccgggcagta gtattttgct tttttcagaa aataatcaaa aaaagttagc
    19621 gtggtgaatc gatactttac cgattgaatt tgcatcaatt tcattaagga atgcgattcc
    19681 actcacaata ttcccgccat ataaaccaag atttaacctt ttgagaacat tttccacacc
    19741 taaaatgcta tttctgcgat aatagcaacc gtttcgtgac aggaatcacg gagttttttg
    19801 tcaaatatga atttctccag atacgtaaat ctatgagcct tgtcgcggtt aacaccccca
    19861 aaaagacttt actattcagg caatacatat tggctaagga gcagtgaaat gcgcgttaac
    19921 aatggtttga ccccgcaaga actcgaggct tatggtatca gtgacgtaca tgatatcgtt
    19981 tacaacccaa gctacgacct gctgtatcag gaagagctcg atccgagcct gacaggttat
    20041 gagcgcgggg tgttaactaa tctgggtgcc gttgccgtcg ataccgggat cttcaccggt
    20101 cgttcaccaa aagataagta tatcgtccgt gacgatacca ctcgcgatac tttctggtgg
    20161 gcagacaaag gcaaaggtaa gaacgacaac aaacccctct ctccggaaac ctggcagcat
    20221 ctgaaaggcc tggtgaccag gcagctttcc ggcaaacgtc tgttcgttgt cgacgctttc
    20281 tgtggtgcga atccggatac tcgtctttcc gtccgtttca tcaccgaagt ggcctggcag
    20341 gcgcattttg tcaaaaacat gtttattcgc ccgagcgatg aagagctggc aggtttcaaa
    20401 ccagacttta tcgttatgaa cggcgcgaag tgcactaacc cgcagtggaa agaactgggt
    20461 ctgaactccg aaaacttcgt ggcgtttaac ctgaccgagc gcatgcagct gattggcggc
    20521 acctggtacg gcggcgaaat gaagaaaggg atgttctcga tgatgaacta cctgctgccg
    20581 ctgaaaggta tcgcttctat gcactgctcc gccaacgttg gtgagaaagg cgatgttgcg
    20641 gtgttcttcg gcctttccgg caccggtaaa accacccttt ccaccgaccc gaaacgtcgc
    20701 ctgattggtg atgacgagca cggctgggac gatgacggcg tgtttaactt cgaaggcggc
    20761 tgctacgcaa aaactatcaa gctgtcgaaa gaagcggaac ctgaaatcta caacgctatc
    20821 cgtcgcgatg cgctgctgga aaacgtcacc gtgcgtgaag atggcactat cgactttgat
    20881 gatggttcaa aaaccgagaa cacccgcgtt tcttatccga tctatcacat cgataacatt
    20941 gttaagccgg tttccaaagc gggccacgcg actaaggtta tcttcctgac tgctgatgct
    21001 ttcggcgtgt tgccgccggt ttctcgcctg actgccgatc aaacccagta tcacttcctc
    21061 tctggcttca ccgccaaact ggccggtact gagcgtggca tcaccgaacc gacgccaacc
    21121 ttctccgctt gcttcggcgc ggcgttcctg tcgctgcacc cgactcagta cgcagaagtg
    21181 ctggtgaaac gtatgcaggc ggcgggcgcg caggcttatc tggttaacac tggctggaac
    21241 ggcactggca aacgtatctc gattaaagat acccgcgcca ttatcgacgc catcctcaac
    21301 ggttcgctgg ataatgcaga aaccttcact ctgccgatgt ttaacctggc gatcccaacc
    21361 gaactgccgg gcgtagacac gaagattctc gatccgcgta acacctacgc ttctccggaa
    21421 cagtggcagg aaaaagccga aaccctggcg aaactgttta tcgacaactt cgataaatac
    21481 accgacaccc ctgcgggtgc cgcgctggta gcggctggtc cgaaactgta atgatttgaa
    21541 gctggagaat atctatccag tatcttatag aaagcaaaac gggaggcacc ttcgcctccc
    21601 gtttatttac ccttcttttg tcgtgccctg cgcccgcgtt accggcactg gcagccaggc
    21661 gcgaatggaa agcccgcccc gctcgctggt gccaagctcc agcatcccgt tatggttatc
    21721 cacgatacgc tgcacaattg ccagccctaa tcccgtgccg ctaatggtgc gcgcactgtc
    21781 gccgcggaca aacggctgga acaggtgctt acgttgttcc ggcgcaattc ccggaccgtc
    21841 atcttccacc tggaaccagg cgcgattcgg ctccgttccg ctgctgactt tgatccagcc
    21901 attgccataa cgggcggcgt tgaccaccat attcgccacc gcgcgtttga tcgacagcgg
    21961 gtgcattttc acgtcaatgc tgccggggta aagcgcggtt tcaatttccc gctcatagcc
    22021 actttcggca gcaatcacct caccgagtac tgcattaaga tccgccattt ccatcggcat
    22081 ctcctgcccg gtgcgcaggt agtcgataaa ctgctcaatg atggcgttgc actcttcgat
    22141 atctttattg atcgattctg ccagatagcc atcctgctcg ctcatcatct cagtcgccag
    22201 gcgaatacgc gtcagcggcg tgcgcaagtc gtgacttacc cccgccatca gcagcgtgcg
    22261 gtcatccgcc agttgcttaa caccagccgc catatggtta aaggcacggg taacggaacg
    22321 cacctcggac gcgccatact cacgcagcgg cggcggaata atccctttac caacctgcaa
    22381 ggctgcgtgt tcgagatcga ccaacggtcg gttctggata cgaataaaca gccacgcccc
    22441 gcctatcgcc aatagcataa tcgccagcgt atagcggaac agcggagaga aatcgccctg
    22501 atgaatttcg gtcagcggca cgcgtaccca gatattgggc gacagccagg ttttcagcca
    22561 gacgacaggc gaacttttgt tgacctcaac gcgcacttcc gtcgggccgc ccagttgctg
    22621 tgccatctga tggcttaaga attcatagtg ttgcgcccaa cgcagacctg cctcttcggc
    22681 agcctcgttg gagtagagag agatccccag ctcacggtag atctcccgac ggaaagcggg
    22741 aggcacaacc aactgcgtgc cgtcctccag ttgcagtttg tcggtcatca acatacgcac
    22801 ttcgtacgcg aggactttat taaactgctg gaggctcggc aaaatcgcga agttcagcac
    22861 caccagataa gtcgtcacca ggctggcgaa cagcaaggtg acgatgagca ataacgtacg
    22921 ggcaaatgaa cttcgtggcg agaagcgcaa tcgcctcatg ctttagagcc gtccggtaca
    22981 aagacgtagc ccaggcccca aacggtctga atgtaacgcg gatgcgctgg atcttcttct
    23041 accatgcggc gcagacgcga aatctgcacg tcgatggagc gttccattgc ggaatattca
    23101 cgaccacggg caaggttcat cagcttatcg cgggagagcg gctcacgcgg atggctgacc
    23161 agtgccttca gtaccgcaaa ctcaccgctg gtgagcggca tcggctcatc ttcgcggaac
    23221 atttcgcgcg taccgaggtt aagtttgaac ttaccgaaag caattaccgc ctcttcctgt
    23281 gacggtgcgc ctggcagttc gttcgcctga cgacgcagca ccgcacgaat acgggccagc
    23341 agttcacgcg ggttaaacgg ttttggaatg tagtcgtcag cgccaatctc caggcctacg
    23401 atacggtcca cttcttcccc tttcgccgtc accataatga tcggcatcgg gttgctctga
    23461 ctacgaagac gtcggcaaat cgacaagcca tcttcaccag gtaacattaa atccagtacc
    23521 ataagatgga aagattcacg agtcagcagg cgatccatct gttctgcatt agcgacgctt
    23581 cgaacctgga agccttgttc ggtgagataa cgttccagca gcgcacgcag gcgcatgtcg
    23641 tcatcgacca ccagaatctt gtagttctct tgcattgttt gtactcccaa aggttcgcaa
    23701 caatttgtaa gcgtgtattc ttaaaaaagc tcacgttcgt caccagctaa atctggtatg
    23761 aatttcagcc taaattgtta caaagcatat taaacagcag cttaagtata caatttattc
    23821 ggcgaaacat tattgattct gttgatatga tcacgttata cccaatgtgc gcattatcaa
    23881 acagacaaag ggaatcaacg agatgaaaac gcccctggtt acccgggaag ggtatgaaaa
    23941 actcaaacaa gagcttaatt atctctggcg tgaagaacgc ccggaggtca caaaaaaggt
    24001 gacctgggcc gcaagtctgg gcgaccgcag cgaaaatgct gactatcagt ataataaaaa
    24061 gcgtctgcgt gaaatcgacc gtcgcgtgcg ctatctcact aaatgcatgg aaaatctcaa
    24121 aatcgtcgat tactcccctc agcaggaagg caaggtcttt tttggcgcat gggtggagat
    24181 tgaaaacgac gatggcgtga ctcaccgttt ccgtattgtc ggctacgatg aaatttttgg
    24241 ccgtaaagat tacatctcta tcgattcccc gatggcccgc gcattgctga aaaaagaagt
    24301 cggcgatctg gcggtggtga atacccctgc cggggaagcg agctggtatg tcaatgctat
    24361 cgagtacgtg aaaccgtaag gaagagtctt aacctcctgc cgatggctgg catttttgcc
    24421 agccagtccg tataactatc ccctgatttt tgatccgaaa agatgaactc aaaccatgat
    24481 gaatgattcg ttctgccgca ttattgcggg tgaaattcag gcgcgcccgg aacaggttga
    24541 cgctgccgtt cgcctgcttg acgaagggaa taccgtgccg tttatcgcac gttatcgtaa
    24601 ggaaatcacc ggcggtctgg atgacacgca gctgcgtaat ctggaaacgc gtctgagcta
    24661 tctgcgcgag ctggaagaga gacgtcaggc gatcctcaaa tccatttccg agcaaggcaa
    24721 actcaccgat gatctggcga aggccatcaa cgccacacta agcaaaaccg aactcgaaga
    24781 tctctacctg ccctacaaac ctaaacgccg cacccgcggg caaatcgcca ttgaagcagg
    24841 gcttgagccg ttggctgacc tgctgtggag cgatccgtca cacacgccag aagtcgccgc
    24901 tgcacaatat gttgatgcag ataaaggcgt ggcagatacc aaagccgcgc tggacggcgc
    24961 gcgctatatc ctgatggaac ggtttgccga agatgccgcg ctgctggcga aagtgcgtga
    25021 ttatctgtgg aagaacgcgc atctggtctc aaccgtgatg agcggtaaag aagaggaagg
    25081 ggcaaaattc cgcgactatt tcgatcatca cgaaccgctg tccacggtgc cttctcaccg
    25141 cgcgctggcg atgttccgtg ggcgtaacga aggcgtactc cagctttcgc tgaatgccga
    25201 tccgcagttc gaagagccgc caaaagagag ctattgcgag caaatcatca tggatcatct
    25261 cggcctgcgc ctgaacaatg ctccggcgga tagctggcgc aaaggcgtgg tgagctggac
    25321 ctggcgcatc aaggtgctga tgcatctgga aaccgaactg atgggcaccg tgcgcgaacg
    25381 cgcggaagat gaagcaatca acgtctttgc ccgtaacctg cacgatctgc tgatggcggc
    25441 ccctgccgga ctgcgtgcca cgatgggcct cgatccgggt ctgcgtaccg gggtaaaagt
    25501 agcggtggtc gatgccactg gcaaactggt ggcgaccgat accatttacc cgcacaccgg
    25561 acaaacagca aaagcagcga tgaccgttgc tgccttgtgt gaaaagcata acgttgaact
    25621 ggtggcgatc ggcaacggta cagcttcccg cgaaaccgaa cgtttctatc tcgacgtgca
    25681 gaagcagttc ccgaaagtga ccgcgcagaa agtgatcgtc agcgaagctg gcgcgtcggt
    25741 ttactcggct tccgagctgg cagcgcagga gttcccggat ctcgacgttt cgctgcgtgg
    25801 tgcggtatct atcgcccgcc gtttgcagga tccgctggcg gagctggtga aaatcgatcc
    25861 gaaatctatc ggcgtaggtc agtatcagca tgacgtcagc cagacgcaac tggcccgcaa
    25921 actggatgca gtagtagaag actgcgtaaa cgccgttggc gtcgatctca acaccgcttc
    25981 tgttccgcta ttaacccgcg tggcgggcct gacgcgcatg atggcgcaaa acatcgtggc
    26041 ctggcgcgat gagaacggcc agttcaagaa ccgtcagcaa ctgctgaaag tcagccgtct
    26101 ggggccgaaa gccttcgagc agtgcgcggg cttcctgcgc attaaccacg gtgataaccc
    26161 gctggacgcc tctaccgttc acccggaagc ctatccggtg gtggaacgca ttctggcagc
    26221 aacacagcag gcactgaaag atctgatggg taacagcagc gaactgcgta acctgaaagc
    26281 gtctgacttt actgatgaaa aattcggtgt gccgacagta actgacatca tcaaagagct
    26341 ggaaaaaccg ggtcgcgatc cgcgtccgga atttaaaacc gctcagtttg ccgatggcgt
    26401 cgagacaatg aacgacctgc aaccgggtat gatcctcgaa ggcgctgtca ccaacgtcac
    26461 caactttggc gcgtttgtcg atattggcgt gcatcaggac ggcctggttc acatctcttc
    26521 attgtcgaac aagtttgtgg aagatccgca taccgtggtg aaagcgggcg acattgtgaa
    26581 ggtgaaagtg ctggaagtgg atcttcagcg taaacgtatc gccctgacca tgcgtctgga
    26641 cgagcagcct ggcgaaacca acgcccgtcg cggcggcggt aatgaacgcc cgcaaaacaa
    26701 ccgcccggca gccaaaccac gcggtcgtga agcgcagcct gccggtaata gcgcgatgat
    26761 ggatgcgctg gcggcggcaa tgggcaaaaa acgttaaacg ccctgattga cccctaaacc
    26821 tgcgcgataa ggtgtccacg ccgcatctgg caacgaacgc cttgtctgac ataaaagtgc
    26881 cggagaatat ctccggcatt tttattccac agccaaactc acaatatatt ccggcaacat
    26941 ttatcacttc attaacagct gaaaccttaa ttaaacatta gccagtcctg gtaattcgct
    27001 attcgaatta tattttcgct gcgatataac cttgagccac atcaacattg agtcagatta
    27061 ttattcaaac caacattcgc acacatttta agtattgctg atagaaacca ttctcattat
    27121 cattgtgctg ttgattattt aatctctcct tcgttggcaa atcatctggt ctcatgtcgc
    27181 tgtcaaacgc cccatgaggt agttatccag ttaatgagaa acaagtaggc acctatgcaa
    27241 tacactccag atactgcgtg gaaaatcact ggcttttccc gtgaaatcag cccggcatat
    27301 cgccaaaaac tgctttctct tggcatgtta cctggctcct cttttaatgt ggtgcgcgtc
    27361 gctccactcg gcgaccccat tcatatcgaa acccgtcgtg tgagcctggt attacgcaaa
    27421 aaagatctgg ccttattaga agtggaagcg gtttcctgtt aatacggtga taacaacaat
    27481 gaaaaaatta accataggct taattggtaa tccaaattct ggcaagacaa ccttatttaa
    27541 ccagctcact ggcgcacgtc agcgtgtagg taactgggct ggcgttaccg tcgaacgtaa
    27601 agaagggcaa ttctccacca ccgatcatca ggtcacgctg gtggacctgc ccggcaccta
    27661 ttctctgacc accatctcat cgcagacctc gctcgatgag caaatcgcct gtcactacat
    27721 tttgagtggc gacgccgacc tgctgattaa cgtggtggat gcgtctaacc ttgagcgtaa
    27781 cctgtacctg acgctacaac tgctggaact cggcattccc tgcatcgtgg cactgaacat
    27841 gctcgacatt gccgagaagc aaaatattcg tattgaaatt gatgctctgt cggcgcgtct
    27901 tggctgccca gtgatcccgc tggtttcaac ccgtggtcgc ggtattgaag cgctcaagct
    27961 ggcgattgat cgctataaag ctaacgagaa tgtggaactg gtgcattacg cacagccgct
    28021 actcaacgaa gcagattcac tggcaaaagt gatgccttcc gacatcccgc tgaaacaacg
    28081 tcgctggctg ggcctgcaaa tgctggaagg cgatatctac agccgcgcct acgccggtga
    28141 agcgtcgcag catctggatg ccgccctcgc ccgtctgcgt aatgagatgg acgatccggc
    28201 gctgcacatt gccgatgcgc gttaccagtg cattgctgcc atctgtgatg tggtaagcaa
    28261 caccctgacg gcagaaccca gccgtttcac cactgcggta gataaaatcg tgctcaaccg
    28321 tttcctcggt ctgccgattt tcctctttgt gatgtacctg atgttcctgc tggctatcaa
    28381 catcggcggg gcgttacagc cgctgtttga cgtcggctcc gtggcgctat ttgtgcatgg
    28441 tattcaatgg attggctaca cgctccactt cccggactgg ctgactatct tcctcgccca
    28501 gggcctgggt ggcggcatta acaccgtgct gccactggtg ccgcagattg gcatgatgta
    28561 cctgttcctc tccttccttg aggactccgg gtatatggcg cgtgcggcgt ttgtgatgga
    28621 ccgtctgatg caggcgctgg gcttgccggg gaaatccttt gtgccgctga tcgtcggttt
    28681 cggttgtaac gtaccgtcgg taatgggtgc acgtacgctt gatgcaccgc gtgaacgtct
    28741 gatgaccatc atgatggcac cgtttatgtc ctgcggcgcg cgtctggcta tcttcgcagt
    28801 attcgcggct gccttcttcg ggcagaacgg tgcgctggcg gtcttctcgc tgtatatgct
    28861 gggtattgtg atggcggtgc tgactggcct gatgctcaag tacaccatca tgcgcggtga
    28921 agcgacgccg tttgtcatgg agttgccggt ctatcatgta ccacacgtta aaagcctgat
    28981 tatccagacc tggcagcgtc tgaaaggctt cgttctgcgt gctggtaaag tgatcatcat
    29041 cgtcagcatt ttcctgagcg ctttcaacag cttctcgctg agcgggaaaa tcgtcgataa
    29101 catcaacgac tcggcgctgg cgtccgtcag ccgggtgatc accccggtct tcaagccaat
    29161 tggcgtgcat gaagataact ggcaggcaac ggttggcctg tttacaggtg ccatggcgaa
    29221 agaagtggta gtgggtacgc tcaacaccct ctacaccgca gaaaatattc aggacgaaga
    29281 gttcaatccg gcagagttta acctcggtga agagctgttc agtgcgatag atgaaacctg
    29341 gcagagcctg aaagacacct tcagccttag cgtactgatg aaccccattg aagccagcaa
    29401 aggcgacggc gaaatgggta ccggggcgat gggcgtgatg gatcagaaat tcggtagcgc
    29461 agcagcagct tacagctacc tgattttcgt cctgctgtat gtaccatgta tctcggtgat
    29521 gggagccatc gcccgtgaat caagccgtgg ctggatgggc ttctccatcc tgtgggggct
    29581 gaatatcgct tactcgctgg caacattgtt ctatcaggtc gccagctaca gtcagcaccc
    29641 aacttacagc ctggtgtgca ttctggcggt tatcctgttt aacatcgtgg ttatcggtct
    29701 gctgcgccgc gcgcgtagcc gggtggatat cgaactgctg gcaacccgca agtcggtaag
    29761 cagttgctgc gcagccagca ccaccggtga ttgccattaa tggcttcact tattcaggtg
    29821 cgcgatttgc tggcgttacg gggccgtatg gaagcggccc agataagcca gacattgaac
    29881 actccacagc caatgattaa cgccatgctg caacaactgg aaagtatggg caaagccgtg
    29941 cggattcagg aagaacctga cggctgcctc tctggcagtt gtaaaagctg cccggaagga
    30001 aaagcctgtc tgcgcgagtg gtgggcgctg cgttaacctt actccatcgc ctgttttgga
    30061 aagcccggta tgcgtctgca tccgggcttt tttgcgtgcg gctttccata aaaatgcaac
    30121 tcttgcagca cggcgtaagt tcctttgaaa gcatctcgca gggatgaaaa ctcgctaata
    30181 cacaggtgtg gagtggcgcg tagagtcgcg gcattcaaac aacaggtgaa ggaacgccat
    30241 gagcaaaaag cagagttcca ccccacacga tgcgctgttc aaactctttt tacgccaacc
    30301 ggacacggct cgtgattttc ttgcgtttca tttaccggca cccattcacg cgctttgtga
    30361 tatgaaaacc ctcaagctgg agtcgagcag ctttattgat gacgatctgc gtgaaagcta
    30421 ttccgatgtg ctgtggtcgg tgaaaacgga acaaggacca ggatacatct attgtctgat
    30481 tgaacatcaa agcacctcaa acaaactgat cgcatttcgc atgatgcgtt acgctattgc
    30541 cgcaatgcaa aatcaccttg atgctggata caaaacgttg ccgatggtgg tgccattgtt
    30601 gttttaccac ggtattgaaa gcccctatcc ctattcgctg tgttggctgg attgtttcgc
    30661 cgatcccaaa ctggcaaggc agctttatgc ctccgcattt ccgctgattg atgtcaccgt
    30721 catgcctgat gatgaaatca tgcagcaccg acgtatggcg ctgctggagt taattcaaaa
    30781 acatattcgt caacgcgatc tgatggggct ggtagagcaa atggcctgct tattaagtag
    30841 tggatacgct aatgacagac aaatcaaagg gctgtttaat tacatactgc aaaccggcga
    30901 cgcggtacgt tttaacgatt ttatcgacgg cgttgccgaa cgttcaccga aacacaagga
    30961 gagtttaatg acaattgcgg aaagattgcg gcaggagggg gaacaatcca aagccctgca
    31021 tatagccaaa ataatgcttg aatccggagt ccctcttaca gacatcatgc gctttaccgg
    31081 ggtgtcagaa gaagagttgg ctgcggcgag ccagtaaatt tctgtctcgc catttcaaaa
    31141 gttgcctaca ccctctgctt caacgccacc agcaggtgac aaaactcgac cggatgcgaa
    31201 ataaacggcg catgggccgc tttggcgaag atatatgatt cgctgtgagg ccaaagttta
    31261 tccagcatcg gcaccacttt gcgcggcacc agaccgtcga gatagccata caatcgcaaa
    31321 aacggcatgg acacgttttg cagcggctga cggagatcga ccgttttcag gatttccagc
    31381 ccgccattaa gcacgtcaac ctccggcatc ggtaacgcca gaacggtttt cttcaacgcc
    31441 cgcgcatcct ggcgcgccgt ttcagtcccc atggtttgta acgccaggaa ccgctccact
    31501 gtacgctgaa aatcatcact gagttgctgc tgaaatcccg ccagtacgtc cggttttatc
    31561 cccggccact cgtcacgagc actaaaacaa ggtgacgacg ccacggtgac cagcgcctga
    31621 acacgctcgg gatgggttaa aacaatttgg cttgccacca gcccgcccag actccagcct
    31681 aaccaaatgg ctttatcagg tgcctgttgc agcacggctt cggccatatc agcaagtgac
    31741 agcgcaccaa atccccggct acgcccgaag ccgggcaggt caacaaggtg cagcgtaaaa
    31801 tgcgagctaa gttcctcgtc aatgcaacgc cacacttcgg cattcagtcc ccatccgtgc
    31861 agcagcacaa gatgaacatt cccctgacct ttggtctgcc accagatgtt attcatccgc
    31921 tattgttctc ttttgactta caaggatgaa catatgctaa cagtaccggg attatgctgg
    31981 ctatgccgaa tgccactggc gttaggtcat tgggggattt gttcggtctg ttcacgcgcc
    32041 acccgcacag ataaaacgtt atgcccacaa tgtggattac ccgccacaca ctcccatctt
    32101 ccctgcggtc gctgcctgca aaaaccgccg ccctggcaaa gactggtcac ggttgccgac
    32161 tatgcgccgc cgttaagtcc gcttatccac cagcttaaat tttcccggcg cagtgaaatc
    32221 gccagcgccc tgtcacgtct gttactattg gaagtcttac acgctcgtcg caccaccggg
    32281 ttgcaattgc cggatcgcat catcagcgtt ccgttatggc agcggcgtca ctggcgtcgg
    32341 ggatttaatc agagcgattt gctgtgtcag ccgttatcac gctggttgca ttgccaatgg
    32401 gatagcgaag ccgtcacacg tacacgggcc actgcgaccc agcattttct cagtgcccgg
    32461 ctgcgcaagc gcaacctgaa aaatgccttt cgtcttgaat tgcccgtgca aggtcgccat
    32521 atggtgattg tggatgatgt cgttaccacc ggaagtaccg tcgcagagat tgcgcagttg
    32581 cttttacgca atggtgcggc gactgtccag gtctggtgcc tttgtcgaac cttgtagagc
    32641 ctcgatgatg ggcgtattat aaccaactaa aatagtcaac tattaggcca ttactatgat
    32701 ccgtatttcc gatgctgcac aagcgcactt tgccaaactg ctggcaaatc aggaagaagg
    32761 gacacaaatc cgcgtatttg tgattaaccc tggcacgcct aacgctgaat gtggtgtttc
    32821 ttattgtccg ccggatgccg tggaagccac cgacactgcc ctgaaatttg acctgctgac
    32881 cgcgtatgtt gatgagttaa gcgcaccata cctggaagat gcagagatcg actttgttac
    32941 cgaccagttg ggttctcagt taacgctgaa agccccgaac gccaaaatgc gtaaagtggc
    33001 agacgatgca ccgctgatgg agcgcgtgga gtatatgctg cagtcgcaga tcaacccaca
    33061 gcttgctggt cacggtggtc gcgtttcgct gatggaaatc accgaagacg gttacgccat
    33121 tctgcaattt ggcggcggct gtaacggttg ttccatggtc gatgtgacgc tgaaagaagg
    33181 gatcgagaag cagctgctga acgaattccc ggagctgaaa ggtgtgcgcg atctcaccga
    33241 acaccagcgc ggcgaacact cctactacta agttatcccc tcttttgtag attgcccgat
    33301 gcgacgctaa agcgtcattt cgggcctacg ttgaacatgt gccagacgtt ggcacagcat
    33361 tgctttggca aatccctccc ttcgtttccc cgcgataata tgaccaacct ctcataattt
    33421 aaatttaccc cgctctggtg attctcaaac gccagatgtt acccgtatca ttcacatggg
    33481 taccaaacat actcctgaca tctgactaca ataattagtt ttagtgggta tcagtcgtgg
    33541 tgccgcaata tctctgttcc cgattgggat aattagagtt tgtcgtcaga aaattgacgt
    33601 tacccataac aaatgaaagg ccaggtaaat catgccatta gtcattgttg ctatcggtgt
    33661 aatcttgttg ttgctcctga tgatccgctt caaaatgaac ggcttcatcg ctctcgtcct
    33721 cgtggcgctt gctgttggat taatgcaagg aatgccgctg gataaagtta ttggctccat
    33781 caaagccggt gtcggcggga cgctcggtag ccttgccctg atcatgggtt ttggcgcaat
    33841 gctgggcaaa atgctggcag actgcggtgg cgcacaacgt atcgccacca cgctgattgc
    33901 caaatttggt aaaaaacaca tccagtgggc ggtggtactg accggtttta ccgttggttt
    33961 tgccctgttc tatgaagtgg gctttgtgct gatgctgccg ctggtgttta ccatcgcggc
    34021 ttctgcgaat attccactgt tgtatgttgg tgtaccaatg gcagctgcac tgtctgtgac
    34081 ccacggcttc ctgccaccgc atccgggtcc gactgcgatt gccaccattt tcaatgccga
    34141 tatgggtaaa accctgctgt acggtactat tctggcaatc ccgaccgtga ttctcgccgg
    34201 tccggtttac gctcgcgtgt tgaaaggtat cgataagcca attccggaag gtctctacag
    34261 cgcgaaaacc ttcagcgaag aagagatgcc gagctttggc gtcagcgtct ggacctctct
    34321 ggtgccggta gtactgatgg cgatgcgtgc gattgccgaa atgatcctgc cgaaaggtca
    34381 cgctttcctg ccggtagcgg agttcctcgg tgacccggta atggctacgc tgattgccgt
    34441 gctgattgcc atgttcacct ttggtctgaa ccgtggtcgt tcaatggatc agattaacga
    34501 cacgctggtt tcttccatca aaatcattgc gatgatgctg ttgatcatcg gtggtggcgg
    34561 tgcgttcaag caggtgctgg tagacagcgg cgtggacaaa tacattgctt ccatgatgca
    34621 cgaaaccaac atttctccgc tgctgatggc ctggtcgatt gctgccgtac tgcgtatcgc
    34681 gctgggttcc gcaaccgttg cggcaatcac tgcgggtggt atcgcggcac cgctgattgc
    34741 aacgacgggt gttagcccgg agctgatggt tattgcggtg ggttccggta gtgtgatttt
    34801 ctctcatgtg aacgatccgg gcttctggct gttcaaagag tactttaacc tgactatcgg
    34861 cgagaccatc aaatcctggt cgatgctgga aaccattatc tcggtgtgcg gtctggtagg
    34921 ctgtctgctg ctgaatatgg tgatttgata acactgactg ccggatgcat cgcatccggc
    34981 atcaaccgca ctctacttct tcttcgctgc agctctgcgc cgtctgtcca aatccttcag
    35041 caacttgttc acgccatcat cggcaaacat cgactcaagc gttgcggaaa gcttgcgtcg
    35101 ccagttttta tactggtaac tggtgccagg aatattcacc ggttcggcca tatccagcca
    35161 gtcttccggc tgtagtccta acagagcact gttactgtcg gcaatgtagc gctgcaaacc
    35221 acggttcagc gtcggcgtca tcgacatcaa cgatgcctta tgcccggcac gttttggcag
    35281 acaaccatat ttatgcagtg catccagcag cccttgcttc gccagttcgc gatcctgata
    35341 cagaccgcgc agtaccactt catccggata cagccccagg gttttgccca gcgttagatc
    35401 cccgctctcc cagtaaccgc gtagcgtagg caggtcatgt gtcgccgcaa ccgccatcga
    35461 ctgctccgga tacgctttcg gtgcacggaa cgtcttctcg tggtcgtttt cgaaatagag
    35521 cactttgtag gagtacacac cgctgctgcg cagcttaccg acaatctcta ccggtacggt
    35581 accgagatct tcaccaatca ccatacagcg atgacgttta ctttcgagtg ccagaatcga
    35641 gagcagatca tccaccggat agtgaacata cgcgccctga tctgccgtct cgccatacgg
    35701 tatccaccac aaacgcagca tcgacatcac atggtcaatt cgtaatgcgc cgcagttttg
    35761 catattggca cgcaacagct cgataaacgg ttcataggca cgcgcggtga tgatatgcgg
    35821 gtccattggc ggtaatcccc agttctgccc caacgggccg aggatatccg gcggcgcgcc
    35881 aaccgacgct ttcaggcaat atagttcacg gtcacaccag gtttccgccc caccttccgc
    35941 tacgccaacc gccagatcac gatacaagcc aatcggcatt tcatagccct ggcttatctc
    36001 ccagcaggcg gcaaactggc tgtaagccag ccactgcaac cagagataaa aatcgacgtc
    36061 atcacgatgt tcttcgcaga actgacgcac ttctggtgaa tccacgttct gatacatctc
    36121 tggccatgca ggccagcccc agcgcatttc gtcctctttc acttgctggg catgtagcgc
    36181 atcaaaggct gcctgccaga acaggctgtc gccctgctct gcaacaaact ggcgaaacgc
    36241 ggtcatctgc tcatcatcac gttgcgcgaa acctttccac gccattcgta atgctgtcat
    36301 ttttagggcg gtaaccgtgg agtaatcgac ccagtcggca tcgcgcgcct gttgcagcgt
    36361 ctgttgcgtg gtcggcaact gccaccaggc ctgagcctct tcgctaagat ggaaatcttc
    36421 aacggcgtta acgtcgatat aaatcacatt cagccaacgg cgagaagacg ggctgtatgg
    36481 gctggcactc tccggatttg ccggatagag cgcatgaatc gggttcaggc cgatgaacga
    36541 cccgccacgt tttgccacat ccaccagcat cgctttgaga tcgccaaaat ccccaatacc
    36601 ccagtttttt tccgatcgca gcgtataaag ctgaacgcag gcaccccaca gcttttgttt
    36661 attcagcaac gcctgcggtt cgtaacagcg tttcggggcg acaatcaccc ggcaatgcgc
    36721 gcgctggtcg tcctgggtga gtgtcagcgt gtgataacct tccggcagct tcgtcggtag
    36781 attgaacgct ttgccccccg ttacatggcc tttgtactgt gttccttctt cggtggtcag
    36841 cagccagcta tattcgccgc tgccctccac caccatcggc atttttttgc cgctggtata
    36901 aaccatgaca ttcgggactg gcgttaccgc cactttcgtg gcggtacgtt gatgcatcgc
    36961 gtcaagcaaa cgccgtttgg tttcggcgct aatcgactgc ggtttaccgt gggcattgat
    37021 gtaattgggg ctaatccccg ccgccagcgc ggcattatcc agacgtttgc tttccatcga
    37081 gcttccttag cgttttgcct gccagatacg agcctgataa tcgcgaatag agcgatccga
    37141 gctaaacata ccgcagcggg cggtattgag gatcgccgcg cgagtccagg cctcctggtc
    37201 gcggtacagc acatccacct gcttttgtgc ctcaacatag gctgcgaaat ccgccatcac
    37261 cagatacgga tcgccgcctt gtttgccgat actgtgcagc atctggtcga aggcatgctt
    37321 atcgccgtcg ctgtatttac cgctttccag ctctttcaat actgcgtcca gcaccttatc
    37381 tttcttccgc catttcaccg ggtcgtagcc tttggccaga atagccttca cttgttccac
    37441 ggtatgacca aaaataaaga tattttcttc accgactttc tcggcgattt caacgttcgc
    37501 accatccagc gtaccgacag taagcgcacc attgagcgcc agtttcatat tgccggtacc
    37561 ggaagcttct ttacctgcag tcgaaatttg ttcggagata tccgccgccg ggatcatttt
    37621 ttccgccgcc gaaacgcaat aatccggcag gaacaccacc ttcaacttat cgccaaccag
    37681 tggatcgttg ttgatcacgt cagccacttt gttgatcgca aagataatat tcttcgccag
    37741 gtagtagccc ggtgccgctt tcgcgccgaa gaggaagacg cgcggtacgc gatcagcctg
    37801 cgggttttca cgaatttctt tgtacaacgc cagaatatgc agcagattca ggtgctggcg
    37861 tttgtactca tgcagacgtt tgatctgaat atcgaaaatc gcctgtggat tgatctcaat
    37921 accggtacga actttcacaa actccgccag acggacttta ttcgcctgct tgatctcgcg
    37981 atattgctga cggaatttcg catcatcagc gaatttttcc agattgatca gctgatcgag
    38041 atcgttagcc cactcttttt gcagtgattt atccaacaga gccgccagtg cagggttgca
    38101 ctgtttgatc cagcgacgtg gggtaatacc gttggtgacg ttatggaatt tgttcggcca
    38161 tagctggtga tattccggga acagatcttt caccaccaga tccgagtgca gcgccgcaac
    38221 accgttcacc gcgaaaccgc caaccacaca caggttcgcc atatgcactt gtttatcgtg
    38281 caccaccgcc agtttggccc acactttttc atcgcccggc caggttttct ccaccagcgt
    38341 tttaaagcga gtattaattt cgttaataat ctgcatatgg cgcggcagta agcctttcac
    38401 cagtttcaca tcccagcgtt ccagcgcttc tggcatcagg gtatggttgg tgtaagcgaa
    38461 agttttgctg gtaatggccc aggcgtcatc ccagctcatc tggtgctcat cgatcagcac
    38521 gcgcagcagt tctggaatcg cgatagttgg gtgggtatcg ttcagctgaa taacttcgta
    38581 atccgccagt tcgtgcagtt tacgccccgc cagatgatgg cgacgcaaaa tatccgctac
    38641 cgaacaggca cactggaagt actgctgcat caagcgcagc tttttaccgg cagtatggtt
    38701 gtcgtttgga tagagaactt tggtcagttt ttccgcattg atgccctgct gttcggcacg
    38761 caggaaatca ccgtcgttaa atttagtcag atcaaacgga tgcgcgtgcg tcgcctgcca
    38821 cagacgcagc ggctgcgcca cgccattacg atagccgaca acggggagat cccacgcttg
    38881 accggtaatg gtaaactccg gctcccagcg tccgtctttc gtcactttac cgccaatccc
    38941 tacctgcaca tccagtgctt cgttgtggcg gaaccacggg tagttactgc gatgccagtc
    39001 atccggcgct tcaacctgtt tgccatcgac aaaagactgg cggaacaaac catattgata
    39061 gttcagaccg taacccgtcg cagactgacc gacagttgcc attgagtcga ggaagcacgc
    39121 cgccagacgt cccagaccac cgttacccag cgccgggtcg atctcttctt ccagcaggtc
    39181 cgtcagattg atgtcataag ccttcaacga atcctgtaca tcctgatacc agccgagatt
    39241 caacaggttg ttgcccgtca ggcgaccaat caaaaactcc attgagatgt agttaacatg
    39301 tcgctgattc gccaccggct tggcgaatgg ctgagcacgc agcatttcgg ccagtgcttc
    39361 actcactgct agccaccact ggcgaggagt catttcagcc gcagaattta agccataacg
    39421 ctgccactga cgtgaaagcg cttcctgaaa ttgcttatcg ttaaaaatag gttgtgacat
    39481 aggagttcca cttttcttag attttcaaca caacgttatc gctagtttgc caggctcgat
    39541 gttgaccttc ctcatcctgc gggggattag gcagggagga gttgcgggga tgagcaagga
    39601 aatgtgatct caaccactta aagctagtgc aaaccacagg attagcagca aatcaatgca
    39661 atacagcgca gaaaatctgt atctaagtgc aaaaaatggc cgttgcgtat tttcaaaaag
    39721 cggaaggtaa ctctataaat taagtaaagg agtgaaacag tttcataagt aaaatatcca
    39781 gtgtgctcca tctcattctt aatagattta ttaagatcat ctttttagat ggcactttca
    39841 tcaggaatga agaagaaacc cttgcttaaa tgaatctgat gaacataagg gaaaccagta
    39901 ttcacgctgg atcagcgtcg ttttaggtga gttgttaata aagatttgga attgtgacac
    39961 agtgcaaatt cagacacata aaaaaacgtc atcgcttgca ttagaaaggt ttctggccga
    40021 ccttataacc attaattacg aagcgcaaaa aaaataatat ttcctcattt tccacagtga
    40081 agtgattaac tatgctgatt ccgtcaaaac taagtcgtcc ggttcgactc gaccataccg
    40141 tggttcgtga gcgcctgctg gctaaacttt ccggcgcgaa caacttccgg ctggcgctga
    40201 tcacgagtcc tgcgggctac ggaaagacca ccctcatttc ccagtgggcg gcaggcaaaa
    40261 acgatatcgg ctggtactcg ctggatgaag gtgataacca gcaagagcgt ttcgccagct
    40321 atctcattgc cgccgtgcag caggcaacca acggtcactg tgcgatatgt gagacgatgg
    40381 cgcaaaaacg gcaatatgcc agcctgacgt cactcttcgc ccagcttttc attgagctgg
    40441 cggaatggca tagcccactt tatctggtca tcgatgacta tcatctgatc actaatccag
    40501 tgatccacga gtcaatgcgc ttctttattc gccatcaacc agaaaatctc accctggtgg
    40561 tgttgtcacg caaccttccg caactgggca ttgccaatct gcgtgttcgt gatcaactgc
    40621 tggaaattgg cagtcagcaa ctggcattta cccatcagga agcgaagcag ttttttgatt
    40681 gccgtctgtc atcgccgatt gaagctgcag aaagcagtcg gatttgcgat gacgtttccg
    40741 gttgggcgac ggcactacag ctaatcgccc tctccgcccg gcagaatacc cactcagccc
    40801 ataagtcggc acgccgcctg gcgggaatca atgccagcca tctttcggat tatctggtcg
    40861 atgaggtttt ggataacgtc gatctcgcaa cgcgccattt tctgttgaaa agcgccattt
    40921 tgcgctcaat gaacgatgcc ctcatcaccc gtgtgaccgg cgaagaaaac gggcaaatgc
    40981 gtctcgaaga gattgagcgt caggggctgt ttttacagcg gatggatgat accggcgagt
    41041 ggttctgcta tcacccgctg tttggcaact tcctgcgcca gcgctgccag tgggaactgg
    41101 cggcggagct gccggaaatc caccgtgccg ccgcagaaag ctggatggcc cagggatttc
    41161 ccagcgaagc gattcatcat gcgctggcgg caggcgatgc gctgatgctg cgcgatattc
    41221 tgcttaatca cgcctggagt ttgttcaacc atagcgaact gtcgctgctg gaagagtcgc
    41281 ttaaggccct gccgtgggac agtttgctgg aaaatccgca gttagtgtta ttgcaggcgt
    41341 ggctaatgca aagccaacat cgctatggcg aagttaacac cctgctagcc cgtgctgaac
    41401 atgaaatcaa ggacatcaga gaaggcacca tgcacgcaga atttaacgct ctgcgcgccc
    41461 aggtggcgat taacgatggt aatccggatg aagcggaacg gctggcaaaa ctggcactgg
    41521 aagagctgcc gccgggctgg ttctatagcc gcattgtggc aacctcggtg ctgggtgaag
    41581 tgctgcactg caaaggcgaa ttgacccgct cactggcgct aatgcagcaa accgaacaga
    41641 tggcacgcca gcacgacgtc tggcactacg ccttatggag tttaatccag caaagtgaaa
    41701 ttctgtttgc ccaagggttc ctgcaaaccg cgtgggaaac acaggaaaaa gcgttccagc
    41761 tgatcaacga gcagcatctg gaacagctgc cgatgcatga gtttctggtg cgcatccgag
    41821 cacaactgtt atgggcctgg gcacggctgg atgaagccga agcgtcggcg cgtagcggga
    41881 ttgaagtctt gtcgtcttat cagccacagc aacagcttca gtgcctggca atgttgattc
    41941 aatgctcgct ggcccgtggt gatttagata acgcccgtag ccagctgaac cgtctggaaa
    42001 acctgctggg gaatggcaaa tatcacagcg actggatctc taacgccaac aaagtccggg
    42061 tgatttactg gcaaatgacc ggcgataaag ccgccgctgc caactggttg cgtcatacgg
    42121 ctaaaccgga gtttgcgaac aaccacttcc tgcaaggtca atggcgcaac attgcccgcg
    42181 cacaaatctt gctgggcgag tttgaacctg cggaaattgt tctcgaagaa ctcaatgaaa
    42241 atgcccgcag tctgcggttg atgagcgatc tcaaccgtaa cctgttgctg cttaatcaac
    42301 tgtactggca ggccggacgt aaaagtgacg cccagcgcgt gttgctggac gcattaaaac
    42361 tggcgaatcg caccggattt atcagccatt ttgtcatcga aggcgaagcg atggcgcaac
    42421 aactgcgcca gctgattcaa ctcaatacgc tgccggaact ggaacagcat cgcgcacagc
    42481 gtattctgcg agaaatcaat caacatcatc ggcataaatt cgcccatttc gatgagaatt
    42541 tcgttgaacg tctgctaaat catccggaag tgccagaact gatccgcacc agcccgttga
    42601 cccaacgtga atggcaggtc ctggggctga tttactccgg ttacagcaat gaacaaattg
    42661 cgggcgaact ggaagtggcg gcaaccacca tcaagacgca tatccgcaat ctgtaccaaa
    42721 aactcggcgt cgcccaccgt caggctgccg tgcagcacgc gcagaaactg ctgaagatga
    42781 tgggttatgg ggtgtgagtt tagccggata atgcattgga tccggtttac atctttacat
    42841 cattcaatac tcacccgcgt tacgccatct gtttctatca aaccaaaccg caccggcaag
    42901 aaacgctcca ccaccgcgat attggtcagc agatggcatg aaggatgggc gaccttaaat
    42961 tctcccgcgc ccgccagcgc catcggcagt accagttggt cggcgagata ttcccccacc
    43021 gccgctgggc ttgccaggta gcgtttcacc tctttcacta actgcgcggc taccacctcg
    43081 gcactgacgc gcttttcacc gacgacgaaa aagcgttcag tgatattttc actttcgact
    43141 tccagcgaga cggtattacc cggcccctgg tcgcgcggca ggttatgaat attctgttca
    43201 tgcagggaaa aactccccgc cagtgtagcg atttcacgct cagcaacatg gcgcggcaca
    43261 ccagctaata gaacttctcc acgcatctgc acaatgttcc cgcgctcgcc aagttgcaag
    43321 gtgttaaacg atgccacggg cgagacttcc gttgccacca cgccgcctcc ggcaggataa
    43381 aaaccgtgac gtaacagcgt ggtttgctga tgaattccta ttttcgccag cagcggctcc
    43441 agcacccggc ggataaaatc cgcaggcggg gccgacgggt tatcggtgcc tccgctcact
    43501 tcaacacgcg aaggtccatc ggcaaaccac agcgcgggca gcaccgtttg cagcaccagc
    43561 gtacagcttc cggcactacc gatagcaaag cggtactcgc cgccgcgcac ggtgccaggc
    43621 cggaagacca gacgctgcga ccccagctcc gcgccttcca ccgttgccct acaaatttcc
    43681 gcagccgctt ttaccgcggt cagatgctgg cgcaacagcc ccggtttcgc ccgcccggca
    43741 cgaatgctgg tgatggtaaa tggctggccg gttatcatcg acaggctcag cgccgagcgc
    43801 aggatctgcc cgccaccttc gccctgtgcg ccatccagcg caatcatcct tttcatcatt
    43861 tatcctttta cgcacaccac ctgacgcagg gtatagataa cttccaccag atcgctttgt
    43921 gccgccatca ccgcatcaat atctttatac gccatcggga tttcgtcgat cacttcggca
    43981 tctttacggc attccacatg cgcggtggca cgaatttgat cttccacgct gaacagtttt
    44041 ttcgctttag tacggctcat tacccgcccg gcaccgtggc tgcacgaaca gaacgactct
    44101 tcatttccca gcccacggac gataaagctt tttgctccca tcgaaccggg aataattcca
    44161 tattgaccag cacgcgcaga caccgcgcct ttacgcgtca cgtagatctc ttcaccaaag
    44221 tgctgttctt tttgcacata gttgtggtga cagttgatct cttccatcgc cagcgtttgt
    44281 ggctgtctga ccgttttctg cgtaatgctc tgcaacgccg ttaccacgtt ttccatcatc
    44341 gcatcgcggt taaggctggc gaaaagctgc gcccaggcca cggctcttag gtaatcatca
    44401 aagtattccg taccttccat aaagtacgcc aggtcgcgcg atggcaacgt ttcaagctgg
    44461 tcctgcatct ctttttgtgc caaatcgata aagtaagtcc cgatggcgtt accaattccg
    44521 cgtgaaccgg agtgcagcat aatccacacc tggtccgact catcaaggca gatttcaata
    44581 aagtggttac ccgttcccag cgttcccagg tgtttatagt tgttagtgtt aaggaaacgc
    44641 ggatattttt gcgttaacca ctgataaccg gcttcaagct cagcccattt cgcatcgacg
    44701 ttaacaggcg ggttttccca tgcgccttta tcacgtttac aacggccagt ggtacgtccg
    44761 tgcggcaccg cagtttcaat cgcctgacgc agctctgcca ggttttcagg caggtctgcc
    44821 gccgttaacg cggtacgcag cgcgttcatt ccacagccaa tatccacgcc caccgccgcc
    44881 ggaataatcg cccctttggt cgggatcacg ctaccaatgg tggaaccttt acccaggtgt
    44941 acatcaggca ttaccgcaat atgtttgaaa ataaacggca tcttcgccgt attaataagt
    45001 tgctgacgcg catcggcttc taccggcacg cctttggtcc acatttttac cggggcattt
    45061 tcagtggtca gtaattcgta attcattttg tttttctctt ttcgttgttt gctgtcgtga
    45121 taattgcaac cgtcgtgcca gaaaattaaa taagcagatt aattttttaa ttcattgttt
    45181 ttaaataggt taaatcttta cgtccgtaaa cggagatttt ccacaaaacc aattcaccga
    45241 taatgaaata tcgtctttta taaggatatc taagatgcgt aaaacagtgg cttttggctt
    45301 tgtcggtacc gtgctggatt atgccggacg cggcagtcag cgctggtcga aatggcgtcc
    45361 gacgctttgt ttatgccagc aagaatcgtt agtcatcgat cggctggaat tgttgcacga
    45421 cacccgctcg cgctcgctat tcgaaacgct taaacgcgat atcgccagcg tttcgccaga
    45481 aacacaagtc gtgggcgttg agattgaact gcataacccg tgggatttcg aagaggttta
    45541 cgcctgcctg catgatttcg cccgtggtta cgagtttcag ccagaaaaag aagactattt
    45601 aattcacatc accaccggca cccacgtcgc gcagatttgc tggtttctgc tggcggaagc
    45661 gcgttacctg cccgcccggc tgatacaatc ttcacctccg cgcaaaaaag aacagccgcg
    45721 cggcgcaggt gaagtgacga ttatcgatct cgatttaagc cgttataacg ccatcgccag
    45781 ccgctttgcc gaggaacgcc agcaaacgct tgattttctt aagtccggca ttgccacgcg
    45841 taacccccac ttcaaccgca tgattgagca gatcgaaaaa gtggcgatca aatcccgcgc
    45901 gccgattctg cttaacggtc caaccggcgc gggcaagtca tttctggcgc gacgcatctt
    45961 cgagttaaaa caggcgcggc atcagtttag cggcgcgttt gtggaagtga actgcgccac
    46021 cctgcgcggc gataccgcca tgtcgacgct gtttggtcat gtaaaaggcg cgtttaccgg
    46081 ggcgcgggaa tctcgtgaag gtttattacg cagcgccaac ggcggaatgt tgtttcttga
    46141 tgagattggc gaactgggcg cagacgaaca ggcaatgctg ctaaaagcca ttgaagagaa
    46201 aaccttttac ccgtttggca gcgatcgcca ggtgagcagt gattttcagc ttatcgccgg
    46261 aacggtgcgc gatttgcgcc agctggttgc cgaaggcaaa tttcgcgaag atctgtacac
    46321 gcggatcaat ctctggacct tcaccctgcc gggtctacgc cagcgccagg aagatattga
    46381 accgaacctg gattatgaag tggagcgcca cgcctcactc actggcgaca gcgtgcgttt
    46441 taacaccgaa gcgcggcgcg cctggttggc ttttgcgacc tctccccagg caacatggcg
    46501 cggtaacttt cgcgaacttt ctgccagcgt cacgcggatg gccacctttg ccactagcgg
    46561 acgcatcact ctggacgtgg ttgaagatga gataaaccgt ctgcgctata actggcagga
    46621 gagccgcccc tccgcgctta cggcgttgct gggcgcagag gcagaaaaca tcgatctctt
    46681 cgaccgtatg caactggaac acgttatcgc tatctgccgc caggcaaagt cgctttccgc
    46741 agccggacgc cagctttttg acgtttcgcg ccagggcaaa gccagcgtca atgacgcgga
    46801 tcggctacgc aaatacctgg cgcgttttgg tctgacgtgg gaagccgtgc aggatcagca
    46861 cagctccagt tgaatatggt ggtccgtcag cacctgcatc acgcttactg gcggcggggc
    46921 gtcggtgtag acggcatcta ccatgctgat gctgcccata ttgaccatcg cgttacggcc
    46981 aaatttcgag tgatcgacaa ccagcataac gtggcgcgag ttctcaataa tggcgcgttt
    47041 ggtgcgaact tcgtggtaat cgaactccag cagcgagccg tcgctatcga tgccgcttat
    47101 ccccagaatg ccgaaatcaa ggcggaactg ggagataaaa tcgagcgtcg cttcgccaat
    47161 gatcccgcca tcgcggctgc gtaattcgcc accggcgaga ataatgcgga aatcttcttt
    47221 taccatcaac gtgttagcaa cattgagatt gttggtgaca atacgcaaat tgctgtgatt
    47281 aagcagcgcg tgcgccaccg cttccggcgt ggtgccgata tcgataaaca gtgtcgagcc
    47341 attggggatt tgctccgcca ctttgcgggc aatcctctct ttttcttcgg tctgggtggc
    47401 tttacgatcg tgccacggcg tgttaaccga actggaaggc agcgccgcac cgccatggtg
    47461 acgcaggatc aggttttgct ccgccagctc attgaggtcg cggcgaatag tctgcgggct
    47521 gacggagaaa tgctctacca gctcttcagt actgacataa ccctgctgtt taaccagttc
    47581 gataataccg ttgtgacgtt gtgtttgttt catttataaa tccctggaat tattttcgtt
    47641 ttcgcgcatt gagcgaatca acaaaagcca tcgctaaacc cacggctaac ccggcgatgt
    47701 gtgctccgtt cgccatcgac atcccaaaca aatcaaacca tccggcgaca atccagatca
    47761 gcgcaaagat aattaaccca cgttgcaggt aaatgccact ttgcggatcg cgttcgccac
    47821 gtagccagac gtagcccatc agcgcataca ccacgccaga aagcccgcca aaccacggcc
    47881 cgctgaattt ttgctgcaca tagccgctta acagggcgct aatgagagta ataacaatta
    47941 gcttaccgct accgaggcgt ttttccaccg caccgccgag ataccaccac cagagcaggt
    48001 taaagaggat atgcatcagc gagaagtgca ttaacgcgtg ggtgaagtaa cgccagaact
    48061 caaatttcag tgttggatcg aatggccagg ccagccataa catcacttcc tgatcgccga
    48121 gaatttgcat ggcgataaac accaccacgc aggcgatcat caccacccag gttaccggac
    48181 ctgcgcgttc acgcaaggca gcaaagaaag gataacggcg ataatgcaga ccactgccgg
    48241 tatggcccgc ctgccagctg gccgccagat aacgcggatc tgccgggttt tcgagaaaac
    48301 gcgccagctc cgcccgcacg cgctcggcct gggactcatc cgccagccag acatcgcttt
    48361 ggttatgttg ttgaatcgtg aggataacac cctgcgtcgc catataatca acaaacgcct
    48421 gcgccacgcg ggggttagca aaagaggtaa tcatcaacat cgttgctgtc gcttattcca
    48481 cacaaaaggg gacagtataa agcgttacgc gccgtacgcc acctctgcgg gaaactgacg
    48541 ctgccaggct tcaaaaccgc cgtcaatgct atagaccaca tcgtagccct gttgcagcag
    48601 atactgcgcc gcgcctttgc tgctattgcc gtgataacac atcaccatca ccggagtgtc
    48661 aaagtcgtta tcacgcataa acgcgcccag cgtgtcgttg gttaaatgga aagcctgcac
    48721 cgcatgcccc atagcgaaac tctgtggatc gcgaatatcg accagcaccg cctctttttc
    48781 ctgcaacttc tggtgcgcgt cggcaacgtt aatacattcg aacagatcca tgcgtctctc
    48841 tttctttaca aacaagtggg caaatttacc gcacagttta cgtcgaagcg gcagataaac
    48901 gccataatgt tatacatatc actctaaaat gttttttcaa tgttacctaa agcgcgattc
    48961 tttgctaata tgttcgataa cgaacattta taagctttaa cgaaagtgaa tgagggcagc
    49021 atggaaacca aagatctgat tgtgataggt ggcggcatca atggtgctgg tatcgcggca
    49081 gacgccgctg gacgcggttt atccgtgctg atgctggagg cgcaggatct cgcttgtgcg
    49141 acctcttccg ccagttcaaa actcattcac ggtggcctgc gctaccttga gcactatgaa
    49201 ttccgcctgg tcagcgaggc gctggctgaa cgtgaagtgc tgctgaaaat ggctccgcat
    49261 atcgccttcc cgatgcgttt tcgcctgcca catcgtccgc atctgcgccc ggcgtggatg
    49321 attcggattg gtctgtttat gtacgatcat ctgggtaaac gcaccagcct gccgggatca
    49381 accggtttgc gttttggcgc aaactcagtg ttaaaaccgg aaattaagcg cggattcgaa
    49441 tattctgact gttgggtaga cgacgcccgt ctggtactcg ccaacgccca gatggtggtg
    49501 cgtaaaggcg gcgaagtgct tactcggact cgcgccacct ctgctcgccg cgaaaacggc
    49561 ctgtggattg tggaagcgga agatatcgat accggcaaaa aatatagctg gcaagcgcgc
    49621 ggcctggtta acgccaccgg cccgtgggtg aaacagttct tcgacgaggg gatgcatctg
    49681 ccttcgcctt atggcattcg cctgatcaaa ggcagccata ttgtggtgcc gcgtgtgcat
    49741 acccagaagc aggcctacat tctgcaaaac gaagataaac gtattgtgtt cgtgatcccg
    49801 tggatggacg aattttccat catcggcacc accgacgtag agtataaagg cgatccgaaa
    49861 gcggtgaaga ttgaagagag tgaaatcaac tatctgctga aagtgtataa cgcgcacttt
    49921 aaaaaacagc ttagccgtga cgatatcgtc tggacctact ccggtgtgcg tccgctgtgt
    49981 gatgatgagt ccgactcgcc gcaggctatt acccgtgatt acacccttga tatccatgat
    50041 gaaaacggca aagcaccgct gctgtcggta ttcggcggta agctgaccac ctaccgaaaa
    50101 ctggcagaac atgcgctgga aaaactgacg ccgtattatc agggtattgg cccggcgtgg
    50161 acgaaagaga gcgtgctgcc gggtggtgcc attgaaggcg accgcgacga ttacgcagct
    50221 cgtctgcgcc gtcgctatcc attcctgact gaatcgctgg cgcgtcatta cgcacgcact
    50281 tacggcagca acagcgagct gctgctcggc aatgcggggg cgataagcga cctcggggag
    50341 gatttcggtc atgagttcta cgaagcggaa ctgaaatacc tggtggatca cgaatgggtc
    50401 cgccgcaccg acgatgccct ttggcgtcgc acaaaacaag gcatgtggct gaatgcggat
    50461 caacaatctc gtgtgagtca gtggctggtg gaatatacgc agcagaagtt gtcgctggcg
    50521 tcgtaaggat gatgcccggt tgaagtgcaa ccgggctatt tggattcctt agactattca
    50581 ttgtaaataa ttgaataacg gcccctgagc taaaatcgcc aaacttaagc acgttaccca
    50641 atcgccccac ttcttcctct ggtttctatt ttttctgcac caatggcaat aatatcccca
    50701 tgcaaaacca agctgtgcac taatcgggcc gacaaaatca gtccaaggct gcggccaaaa
    50761 aggggaaaaa ttcaggggag gccagacaaa cgtcagaaag aaatttcgga tattttccag
    50821 caacgctgat ttctctggag aaatataaag aatgaaattc gagagaatta accagaaata
    50881 gaccggcgaa aagaccagcc agcaatgcag tctgtttttt aattcaggag gcgttctgcg
    50941 ggagaattgc caaccgatgc cagccattac actgaaaaaa agtgcatatt caaccattaa
    51001 ctcaagttgt aattcctgaa gagaaatacg cacaaccagg agcggaaagg agaatattat
    51061 ttctgccaga aaagttaacg tgaatataag tacggctaag acaataaccg tcggccatac
    51121 caataatggc acgataacaa aaccgagaat tacaggggga atataaagcc ggaatttaat
    51181 catttttatt ttcctggtga ttcggatgat gcgtcataca tcgcgcataa acactcatca
    51241 attatccagt catcttccca gacctgatat tctgtcattg gttcgggctg ttttagcggg
    51301 ttctcaatcg cttgccgcgc tctttctttc agtaaaggat gattatttct agcccaggca
    51361 ttgatttggg tggtaatggc gttatcgata tcgtcctggt tatctctgat ataatcctca
    51421 caatatctgt tcaataaaca ggcaagagaa acggtcgacc aaaagccggg aactgaatta
    51481 atggcatcca gcaatggcgc aagagcgata tcggcacgga aaaacatttc cagcatatac
    51541 tgtaattcga cagcttcatc gccggatacc caatcagtca tatactggca ggcaaatcgc
    51601 tgtaaaattg cccgttcatc cgcacgccag ttttctttgt tggcaaggtt gatacggcgc
    51661 agagaaaaaa ctacgtgaat attgtcaaaa tgcccctgtg caacaaattc cagtaaacgc
    51721 ggcagaaaat agcggacctc atcgctattt tgcggatcag gccctgggct actattccag
    51781 gcattgatta aggaatacgg gatcgctcgt aacgatgtac tccgtaatgc cttttgttcc
    51841 tgctgactca ggcaatattc acaacaaacc acaaatttct gcggcacccg ataacgacga
    51901 aaggtgtgat aaatctgctc aattaatgcc tgcatggcgg ggctgggttg catagttacc
    51961 ccctcgcccg ctgatcgcgt gtaaacggtt gatagacaaa acgagcattc gccgcgttca
    52021 ggcgaataag ggcgctacgc gaaagggaaa agctggggcg agtgcaacta ttgcagtctc
    52081 gacagctttc ctctgcgcaa ttttccactg agtaatgacg cgtgtagagt ttcagttcgg
    52141 cctgcttatc gcgaaatccg cgtaaactgt tattgttatg aaatttccag gcatctttaa
    52201 agacctgaaa gatatagaca tcctcaatcc aggagtcaca ttttttaagc gggcgctttg
    52261 ctattatcac ccgctcatcc ggatttagca tcgccgtaaa ttgtacggtg acgtcatttg
    52321 tcgtgttcag gtcacattga acatctggta catcactgat ataggggtca aatccaaaat
    52381 cagcaccaca ggcacaaaga cggctaatgg tggcggtatc gagggaaaat cccggccata
    52441 ccttgctgga ggttaagcca atatttaatg tgacatcata tcgaagcaat ccctcaagac
    52501 cgtcagcacc gacgaacttt tccccttcat ccagggcctg gttaataagt tgctgaacaa
    52561 tgccttcatc ccagtatgcc gatttgtagt tggcaatcgt aatatccacg cctgtgaagg
    52621 gatcagtacc ggcaaggctt gcaaataatg tattaactaa ttccattttt attatccagc
    52681 tttaaggtag cgtcttatac atcgggacat aacctacatc ttccaccaaa ctctgtccct
    52741 gcggcgttaa aaaccactcc aacaattttt gtgtctctga cgtcgtattt tcccgcgtta
    52801 tcataaaggc atcgacgata tagggatatt tgccgttgcg aatgttttcg gcagtcggtg
    52861 caataccgtt aatcgctagc agttttatat ttttatcggc gttcatttgt gttgcgtaat
    52921 aacggaaggt gtaacctatt gcgttgtttg tattacggta ttcggcaacg actttaatca
    52981 ttccctccat catacttgcc acttctgttt cctgcggcga gatcatgcgg acatttttca
    53041 tgacctggga ttgcatcacc gtctggctac cagagtcttc cgggcgttgc caggtctgga
    53101 tctcctgatc gttaccgcca acagtacgcc agttggtaat tgcaccactg aagatgtcac
    53161 gcacctgttg ttcagtcagg gaattaaccg gattatccgc attgacgatg aaaacaaacg
    53221 cttcacgggc aaaaggcgtg tacagcaaag taacgcccga ttcctcagcg cgttttttct
    53281 gcccaccgga aggttgcgcc acaaaaataa tatcagcatc acctttaaca atcctgttat
    53341 acgcatcggg ggtacgagag ctctccagat attccctggt gtgaaaatct tccggtataa
    53401 cgcttaaagc ataaaatgca gaagcataaa ttgggtacgc cgccgtagca ccatcgagtc
    53461 gcggccagtt ttgcgtaaac tgaatttgcg gtttaccacg cagtggtgtc agttgattat
    53521 tgagtttatc gggtcgccag gcccaggtat caatattttc gcgcacaccg acgccgggat
    53581 tgagctgttt atataaataa ccctgataac cagcgacaaa accaagcatg agaaggataa
    53641 acaggaggga attacggcaa cgtagcccgg atgtaccggt taaaggatgt ctggttggcc
    53701 ggccgtaccc cagcgaaaaa ctgatatgcg cgccaacggg tataatgact atccaccagt
    53761 actcacctcc gactactgag actaacaaaa cgcccagcca ggggagcata agatcagcca
    53821 accaacccga tcccatctgg tgaaattgat aatctgccag atgcagcgcg attatccagc
    53881 caaccaggat taataaaccg ggtactaaaa aaagacattg tcgccagacg gctatttctc
    53941 ttttttctgg tgcactatcg cctgcccata ctcgccccga gaaaaatagc attaatgcgc
    54001 ccagcgccag cataaaaatg ttgagcagaa ggtaacttgt tggcgtgata aggatattac
    54061 aaacttcaat aataccggca agtccgacac ccagcatagc aacaaccgcc gccaaaaatt
    54121 gcgtcagtat ggggatgccg aaaaaagtca ttaccagcga ggacaaaatc cattttctgt
    54181 tttgcattat tctttccatt ctttttgaat ggtgaaatta tactccccga gtccccttgc
    54241 cccttctgga cacttttccg aaatgatggc ggaaaaaaac gggagccttt ggccccgttc
    54301 tatttattgg tgaacttaca atctcaccgg atcgatatgc cagatatgat cggcgtactc
    54361 tttgatagta cggtcagaag agaagtagcc catattggca atgttcagca tcgcttttgc
    54421 ggtccactct tcctgaagct cgtagagttc gtcgacttta tcctgacaat cgacatagct
    54481 gcgataatcc gccagaacct gatagtgatc gccgaagttg atcagcgaat caaccagatc
    54541 gcgatagcga cccggatctt ccggactgaa tacaccgctg ccgatttgcg tcagcacctg
    54601 atgcagctcc tcatctttct cgtagtattc acgcggtttg tagccctgac gacgcagttc
    54661 ttccacttct tccgcagtgt taccaaagat aaagatattg tcagcaccga catgatccag
    54721 catctcgacg ttcgcaccgt ccagcgtacc gatagtcagc gcgccgttaa gcgcaaactt
    54781 catgttactg gtgccggaag cttccgtccc tgccagcgaa atctgttcag acagatctgc
    54841 cgccggaatg atcaactgcg ccaggctaac gctgtagttc gggatgaaca cgactttcag
    54901 cttatcgcca atctgcggat cgttgttgat cactttcgct acgtcattga tcaaatgaat
    54961 aatgtgcttc gccatgtaat aggccgaagc cgccttaccg ccaaaaatat tcacgcgcgg
    55021 tacccacttc gcatccgggt cggccttgat gcggttatag cgggtaatca catgcaacac
    55081 attcatcaat tgacgtttgt attcgtgaat acgtttgatt tgtacatcga acaacgcctt
    55141 tggattcacc accacattca gctgctgggc gatatactct gccagacgct ttttgttctc
    55201 cagcttcgcc tgatgcacag cgtgattaac cattgggaaa tcacagtgtt gttgcagctc
    55261 attaagcagg ctaaggtcgg tgcgccagtt acggcccagg tgttcgtcca gcacggctga
    55321 aagcgatggg ttcgctaccg ctagccagcg acgcggcgtc acaccgttgg tgacgttggt
    55381 gaaacgaccc gggaagattt tcgcaaagtc ggcaaacaac gattgcacca tcagattaga
    55441 gtgcagttcc gatacaccgt taactttgtg gctcacaaca accgccagcc aggccatacg
    55501 cacacgacga ccgttggatt catcaatgat cgacgcccgt cccagcagat cggtatcgtt
    55561 cggatactgt tcctgcaagg ttttcaggaa atagtcgttg atttcaaaga tgatctgcag
    55621 gtgacgcggc agaattttac ccagcatatc aaccggccag gtttccagcg cctcgctcat
    55681 cagcgtatgg ttagtgtagg agaagacctg acaacacacc tcaaacgcgt cgtcccagct
    55741 aaattggtgc tcatcgatca gcagacgcat catctcagga atcgacagta ccggatgggt
    55801 atcattgaga tgaatcgcga ttttatccgc caggttatcg taggttttat gcaactgata
    55861 atggcggctt aaaatgtcct gaatggtcga ggaaaccagg aagtattcct gacgcaggcg
    55921 cagctcacgc ccggagtagg tggagtcatc cggatacagt acgcgagata cgttctcgga
    55981 gtggttttta tcttccactg ccgcgaagta gtcaccctgg ttgaatttac cgaggttaat
    56041 ttcgctactg gcttgcgcac tccacaaacg caacgtgttg gtcgcgtcgg tgtcgtaacc
    56101 agggattatc tgatcgtaag cgactcccag aatctcttcg gtttcaatcc agcgcgtttt
    56161 tttaccttcc tgctgaatgc gaccaccaaa acggacttta tagcgcgtgt tgtggcgttt
    56221 gaattcccac gggttaccgt attccagcca gtagtctggc gactctttct ggctaccgtt
    56281 aacgatgttc tgcttgaaca taccgtagtc atagcggatg ccgtaaccgc gccccggcaa
    56341 ccctaacgtc gccagagaat caaggaagca agccgccaga cgtcccaggc caccgttacc
    56401 gaggcctggg tcattttctt catcaatcag ctcttcgaga tttaacccca tcgcttccag
    56461 tgcgccctgt acatcttcgt aaattcctag cgacaacatg gcgttggaga gcgtacggcc
    56521 aatcaaaaac tccatcgaca ggtagtaaac ctgacgagtt tcttgcgaca actgggcacg
    56581 gtttgaacgt aaccagcgct ccacgagacg atcgcgcaca gcaaataacg ttgcgttcag
    56641 ccattcatgt ttattggcga cgaccgggtc ctttccaatc gtaaacatca gcttgtaagc
    56701 gatagagtgc ttaagagctt ctacgctaag cgtgggcgat gaatatgtaa acggagcatt
    56761 catataggcg tttcctgaaa actatttcaa gcgatagtaa agctcacggt acgacttcgc
    56821 cgcgacctgc cagctaaaat ccattgccat agcctgacgt tgcacaaacc gccacagtga
    56881 aggacgggac cacagtacaa aagcacgtcg aatagcccgt aacagcgacc aggcattact
    56941 atcttcaaag acaaacccac tggcgacgcc atctgcaagg ttctcaagag aacagtcaga
    57001 aaccgtatca gcaagcccac cggtgcgccg cactaacggc agcgtaccgt acttcaatcc
    57061 ataaagttgc gttaagccgc acggttcaaa acggctgggc accagaatga cgtccgcgcc
    57121 gcccataatg cgatgcgaaa atgcttcgtg atagccaatc tgaacgccca cctggccggg
    57181 gtattccgct gccgccgcaa ggaaaccttc ctgcagcacc ggatcgcccg cgccgagtag
    57241 cgccagctgc ccgccctgct ccagaaggcc cggtaaggct tccagcacca ggtcgagacc
    57301 tttctggctg gtcagacggc tcaccactgc aaaaagcggc actttatcat caaccttaag
    57361 ccccattgcg atttgtaact ggcgcttatt ttccgcttta tcttccaacg tatcgcgggt
    57421 gtagcgcgag gccaacagta agtccgtctc tggactccag attttctcgt ccacgccgtt
    57481 cagtacgccg gaaagacgcc cttcacggtg acgctgttgc aacagacctt ccataccgta
    57541 ggcaaactgc ggttcggtga tctcgcgagc gtaggttgga ctaaccgccg taatgtgatc
    57601 ggcatagtac agaccggcct tcaggaaaga gatttgtccg ttgaattcca gcccatgaat
    57661 attaaagaat gaccatggca attggatgtc attcatatga tgtgcataaa acatgccttg
    57721 ataggccagg ttgtgcacag taaacaccga cttcgccgga cgcccgcgcg ccgccagata
    57781 cgcaggcgca aggcctgcat gccagtcgtg cgcatgcacc acatcaggac gccagaatgg
    57841 gtcaagcccg ctggccattt ctgccccaac ccaccccagc agcgcaaaac gcaatacgtt
    57901 gtcggtatag gcaaataagt tggtatcgtg atacgggctt cccggacgat catagagatg
    57961 cggcgcgtca atcaggtata tgccaacccc gttgtaatga ccgaacaaca gcgtgatatg
    58021 tccggcgaag gtatcacgac gggatactac ctgcgcatcg gtaacgccac ggcgaatatc
    58081 gggaaatgca ggcaacagta cgcgagcgtc aacgccgtct gcgatttgtg ctgcgggtaa
    58141 tgccccaata acatcagcca gaccgccggt tttaagcagc gggaacatct ctgaacatac
    58201 atgtaaaacc tgcattatcg ctcctgttta tgccctaact tccgtagcat ttcgcgcgtt
    58261 accagcacga tgccttcttc tgaacgatag aaacgacgtg catcttcctc tgcgttttca
    58321 ccaatcacca tgccttccgg aataacacaa gcacgatcga tgacgcagcg gcgcagacgg
    58381 cacgagcgac ctacccatac ttccggtaac aatacggcgg aatcaatgtt gcagaatgaa
    58441 ttcacgcgaa cgcgcgagaa cagaacggac tgcaccacca ccgaaccgga gatcacacag
    58501 ccgccggata ccagtgagtt aagggtcatc ccgtggctac cggagcgatc ctgcacgaat
    58561 ttcgctggcg gtaatgattc attgtaggtg cgaattggcc aattgcgatc gtacatatcc
    58621 agttccggca ccacagaggc cagatcgagg ttcgctttcc agtaagcttc cagcgtaccc
    58681 acatcgcgcc agtacggctc ggcatccggg tcggattgta cgcaagagag cgggaacggg
    58741 tgcgcatagg ccagaccggc ttcggtgatc ttgggaatca aatctttgcc aaagtcgtgg
    58801 ctggagttct catcacgatc gtcttcttcc agcagttcat acagatagtc ggcgtcaaag
    58861 acgtagatac ccatactcgc cagagatttg ctcggatcgt tcggcattga cggtgggtta
    58921 gcaggttttt caacgaattc gataatttta tcgttctcat caaccgccat aacgccaaat
    58981 gcggaggctt cttcaatcgg tactggcata caagcaacgg tgcaacgtgc gcctttttcg
    59041 acgtgatcga taagcatacg cgagtagtct tgcttgtaga tatggtcgcc cgccaggatc
    59101 accacgtatt ccgctttata acggcggata atgtcgaggt tttgggtgac cgcatctgcg
    59161 gtgccgcgat accagttttc ccctttcatt ctctgctgtg ctggcagcag atcgacaaac
    59221 tcgttcattt cttcattgaa gaatgaccag ccgcgctgaa tgtgctgcac cagagtgtgg
    59281 gactggtact gggtgatcac gcccatacga cggatcccgg agttgatgca gttagacagc
    59341 gcaaagtcga taatgcggaa cttaccgccg aagtgtacgg ccggttttgc tcgcttattg
    59401 gttaaatcct tcaggcgggt accacgtcct cccgccagta tcagggcaac agatttcaat
    59461 ggcagctggc gcgccaacat taagtgatcg ttcttctcta aactaaccat gactaactcc
    59521 ttttttatca tctctggaac acacacaatc cgtgtgcagg tccctgccag acagccgtaa
    59581 tcactgggtt atcctctcca gcgaatgggg gaatggcgtg ccactcccca gcaggtaaaa
    59641 caatctctgt tacctcaagc gtggcgttaa ttgcgatcaa aaagcgatcc gagagcagaa
    59701 tttgcagctg tttcggcccg ttttgccact catccgtgct taaaggttga gcatatcgat
    59761 ttagccaacg gacattgccg tcgccttctt cccaccagcg attctccacc aaagcgggaa
    59821 tgcgcttgcg cagatggatt aacgcggcgg taaatgcggt taaaccactg cttgcctgcg
    59881 accagtccaa ccaggttaat tggttatcct gacagtaggc attgttattg ccatgctggc
    59941 tgtgaccatg ttcgtcaccg gccagtaaca tcggcgtacc ctgggagagc aacaacgttg
    60001 ttaacagggc gtgaatgctg tcgcgccgcc gttcaaccag gtcaagagaa ccgcctaacc
    60061 cttctttacc atgattgtta ctgtaattgt tgttggtccc gtcgcgattt tcttctccgt
    60121 ttgcttcatt gtgtttatgg ttgaagcaaa cgcagtcgcg aagcgtaaaa ccgtcatgcg
    60181 cggtgacgag attaatcgcg gcactcggca gacgaccatt acgtttaaaa acatcgctgg
    60241 aggcagcaaa acgcccggca aacgccccca gaggcaaatc ataatgtagc cagaaacgac
    60301 gggcagcatc gcggaaatga tcgttccact cggcaaacag cggcgggaaa tttcccacct
    60361 gataaccacc aggagcgata tcccacggtt cagcaattaa cttcacctgc gagagcaccg
    60421 ggcagttctg gatagcggta aacaacggcg catcctgacg gaactctggc gtacggccca
    60481 tgactgccgc cagatcaaag cggaaaccat cgacgtggca ggtttctacc caataacgca
    60541 ggcaggcgct ggcataatcc accaccgccg gatgactcaa attgagcgtg ttgccgcaac
    60601 cggtccagtt gtgataatcg ccgtcttctc ttatccaata atagctacgg ttatcgatcc
    60661 cacgcagcga gaataacggg ccgtcgaggt ccagttccgc actatggttg agcacgatat
    60721 caagaatgac ttcgataccc gctttatgca gtgctttgat tgcatcgcga aactcatcca
    60781 gcgccgtttc tggcgagcag gcatacgccg gatgcagcgc aaacatcgcc accgggttgt
    60841 aaccccagta gttacttagc cccatgcgtt gcagacgtgg ttcactggca aactgcgcca
    60901 ctggcagcag ttccagcgcg gtaatgccca attgtttcaa atagttgatc atcaccggat
    60961 gcccgagggc tttataagtg ccacggatct cgaccgggat ctccgggtgc aagtacgtta
    61021 atcctttgac atgggcttca taaatgatgg tgctgcccca cggcgtgcgc ggcggggcat
    61081 catcttccca gtcatagtga tcaaccacca ctacgcattt cggcgcaatg gcggcgttgt
    61141 cgcgatagtc aggttcatta tgaccggcgt gcagcagcgg gttatcttta aactccccgt
    61201 caatttgccg cgcgcaagga tcaatcaaca acttcgccgg gttaaagcga tgcccctcgg
    61261 cgggttgcca ggggccatga acgcgataac cataacgcaa acccgggcgc gcatccggca
    61321 gataaccgtg ccaaatgtcg ccactgtgcc ctggcaagtc atagcgatgt tcctggccat
    61381 tggcgtcaaa gacacacagt tctacccgct cggcatgagc ggagaaaagt gtgaagttga
    61441 cgccctgacc gtcgtaatgc gcgccgaggg gagcgggttt gccaatggcg agttgtgtca
    61501 ttctgcctcc cgaaccagcc agatagtggc cagcggtggt agcgtcaggc ttagtgaatg
    61561 ctgacgaccg tggctggcaa tctcatcgct gtgtaccgtg ccgccattgc ctgcattact
    61621 gccgtgatag tgcatggaat cggtattgag gatttcacgc catttgcccg gctggtttat
    61681 gccgaagcga taatcatgac gcggtaccgg cgtaaagtta ctggcaacga tgatttcgtt
    61741 accctcttta tcgcgacgca caaagatcag caccgagcgt tctttgtcat ccaccaccag
    61801 ccattcaaag ccgtacgggt caaaatccag ttcatgcatt gctttatggt ggcggtaggt
    61861 gaggttcaga tcgcgcacca gacgctggac accgtggtgc cagttatcgc cgccttccaa
    61921 cagatgccag tcgaggctgg cgtcatggtt ccactcgcgg ccctgggcaa attcgttacc
    61981 catgaacagt agtttcttgc ccgggaatgc ccacatccag ccatagtagg cgcgcaggtt
    62041 cgcgaatttc tgccatgcgt cgcccggcat gcggtcgaga atcgattttt taccgtggac
    62101 cacttcatca tgcgacaacg gcaggacgaa gttttcagtg tagttgtaga gaatcccgaa
    62161 ggtcagttta tcgtgatgat actgacgata aaccgggtcg agcttcatgt agtccagggt
    62221 gtcatgcatc cagccgaggt tccacttgta ccagaagccc agaccgccca tatcctgcgg
    62281 acgagaaacg ccagggaaat cggtagactc ctcagccatt gtcaccgcac cggaaacctg
    62341 ctcaccaaga atacggttgg tattacgcaa gaattcaatc gcttcaagat tctcgcgccc
    62401 gccaaattcg ttcgggatcc actccccctc tttacggctg tagtcgcgat aaatcattga
    62461 cgccaccgca tcgacgcgca gcgcatcaat accaaaacgt tcaatccagt aaagcgcgtt
    62521 accgacgagg aagttactga cttcacggcg accatagttg tagatcagcg tgttccagtc
    62581 ctgatgatag ccttcacgcg gatcgctgtg ttcatacaag ttcgtgccat caaattcggc
    62641 aagcgcaaag tcatcagtcg ggaagtggcc tggcacccag tcgagaatca cgttcagacc
    62701 agctgcgtgt gcggcatcaa tgaaataacg gaagtcgtcg cgagtaccaa aacggcgggt
    62761 tggtgcatac aggccggttg gctgataacc ccaactgcca tcgaagggat gctcgttaat
    62821 gggcagtagt tcgaggtggg taaagcccat ccatttagca taaggcacca gttgatcggc
    62881 cagctcgcgg tagctcaacc agaaattgtt gtcggtgtgg cgacgccagg aacccaggtg
    62941 aacttcataa atagagattg gcgcatcaaa ctgattcgct tttttgcgct cttcagtctg
    63001 tacaaccttt tccggcagcc cgcaaataag agacgcggtt tccgggcgca tttgcgcttc
    63061 gaaggcataa gggtcggact tcagacgcaa gttgccattg gcatcaatca tctcgtattt
    63121 atagagctga ccgttatgcg ccccagggat aaacagttcc cagatgccgc tctctttacg
    63181 caggcgcatc gggtgacggc gaccgtccca gtagttgaat tgcccaacca ccgagacccg
    63241 acgggcgttt ggagcccaga cagagaaacg cgtacctgtg acgccatcca tagtatctgc
    63301 atgcgcgcct aaggtttcat acgggcgcag gtgagtacct tcagataata gccaggcatc
    63361 catttcctgg attagcggac caaaacggta aggatcatca atcaggtttt gctgaccatg
    63421 ccagacaaca gccaactgat agcggaaaaa attcttacgt cgcggaatga cgccgctaaa
    63481 gaatccccgt gagtcgagac actccagttt tgcgagtttg cgcccggttt tcggttcaat
    63541 cacccacaca tcggtagcgt cgggtaaaag ggcacggact tccagtcccg cggtggtttt
    63601 atgcattccc agtacggaaa aaggatccgc aaaatggcct gcaattagcg cgttaatcac
    63661 gtctctatcg atacgatcgg acatgcttgt cttcctgttt tattgtgtca ccccatccaa
    63721 ttggattttt gacttctggt tgtgacattt ttttgacctg aacggcgcag cactctgtgc
    63781 atcctctctg cgtcgtcctc acttcaggta aggctgtgaa tactcatgta ttcagccacc
    63841 cttaaagaat agccaatgct ctatttaact cccggtaaat catgaaacat ctgcgcttac
    63901 tcctgtatta cgcactaaca ggggcggcat cgcgccccag atttaatgaa taaagattac
    63961 gccagttgac gaagcatccg acgcagcggc tccgcggccc cccacagcag ctggtcgccc
    64021 acggtaaagg ctgacaggaa ctctggtccc atattcagct tacgcaggcg gcctaccggc
    64081 gtggtcagcg tgccggtaac ggcagctggg gttagctcac gcatagtgat ttcccgatcg
    64141 ttcggaacga ctttcgccca cggattgtgc gcagccagca gttcttccac ggtcggaata
    64201 gacacatctt ttttcaattt aatagtgaat gcctggctgt ggcagcgcaa tgccccgaca
    64261 cgcacacata aaccatctac cggaattacg gaagatgtgt tgaggatctt gttggtttcc
    64321 gcctgccctt tccactcttc gcggctctga ccgttatcga gctgtttgtc gatccacgga
    64381 atcaggctac ccgccagcgg cacgccaaag ttatccaccg gcagctcacc gctacgggtt
    64441 aaggttgtga ctttgcgttc gatatcgaga atagcagagg acggggtcgc gagttcatct
    64501 gccacatggc catacagatg gcccatctgg gttaataact cacgcatatg tcgcgcacca
    64561 ccgccggaag cggcctggta ggttgcaacg gacacccaat caacaagatc attggcgaat
    64621 aaaccaccca acgacatcaa catcaggctt acggtacagt taccgccaac aaaagtcctg
    64681 atgccattat ttaatccgtc ggtaatgacg tcctgattga cggggtcaag aatgatgatg
    64741 gcgtcatctt tcatgcgcag agacgatgct gcgtcaatcc agtaaccttg ccatccgctt
    64801 tcacgaagct ttggatagat ttcgttggta taatcgccgc cctgacaggt cacaatgata
    64861 tcgagggcct ttagcgcctc cagatcaaag gcatcctgaa gtgtgccagt ggttccgcca
    64921 aaagacggcg cagcctggcc aagctgagaa gtagaaaaga agacagggcg aatggcgtcg
    64981 aagtcgcgct cttcaaccat gcgttgcatg agaacggagc cgaccatacc gcgccagccg
    65041 ataaaaccaa catttttcat aagcgttttt tcctgcaaag atgtgtgctg tataaatgtg
    65101 ccggtctcct cttggcacat ctttcaccat acaaaaagca gccaaagtcg caagtgaaat
    65161 taatcaatga tagcgaagcc atcagtaatg cgacttatcc tgctttgtta gcacgcagaa
    65221 agtccgcggc aattatcagg gaatttgagt tatgaatgaa atcatttctg cagcagtttt
    65281 attgatcctg attatggatc cgctcggaaa cctacctatt ttcatgtccg tactgaaaca
    65341 tactgaaccg aaaagacggc gggcaatcat ggtgcgagag ttgcttattg ctctcctggt
    65401 gatgctggtg ttcctgtttg cgggtgagaa aattctggca tttctcagcc tgcgagcaga
    65461 aaccgtctcc atttctggcg gcatcattct gtttctaatc gccattaaga tgattttccc
    65521 cagcgcttca ggaaatagca gcgggcttcc ggcaggtgaa gagccattta tcgtgccgtt
    65581 ggctattccg ttagtcgccg ggccgactat tctcgccacg ctgatgttgc tgtctcacca
    65641 gtacccaaat cagatgggac atctggtgat tgctctgctg ctggcctggg gcggcacctt
    65701 tgtcatcctg ctacagtctt cgctattttt acgtctgctg ggcgagaaag gggtgaacgc
    65761 acttgaacgc ctgatgggat tgattctggt gatgatggca acccagatgt tcctcgacgg
    65821 cattcgaatg tggatgaagg ggtaacgagg agagtttttc gaaaagaacc cggtagcgtc
    65881 acaactaccg ggcgaacaaa cttaactcaa cagctggaac gcaatcatcc caacgatggc
    65941 accgacagtg ccgaggatgg tttccatcat ggtccaggtt ttcagcgttt cggcttcggt
    66001 cgcgccggta aatttaccga acaaccagaa accggcgtcg ttaacgtggc tgacaacaat
    66061 cgaaccacca gcgatacaaa tcgacagcgc cgccatttgc gcaccggagt agttcagttg
    66121 ttcaataacc ggcatcacca gtcctaccgc cgttaaacag gctacggtgg cagaaccctg
    66181 aatgatgcgc actgcagcgg ccagcacgaa gcaggtgatg gctatcggca ggcccatgcc
    66241 agttaacgct tcgcccagtg ccggacctac gccagagtca accagcacct gtttgaacac
    66301 gccacctgca ccaatcacca gcagaatgat ccccgccggt tgcagcgcgt gaccgcaaat
    66361 ctccatcact ttgtcttttg gcatgccctg acgcattgcc aggccataaa tcgctaccag
    66421 acaagcaacc agaatcgcgg taaacggatg accaataaac tcgaaccatt cgtaagcggt
    66481 agagccttct ggcacaaaac gcgcggcaat ggttttcagc cccaccagca ccagcggcaa
    66541 caggatcagc gacaggctga atccgaaaga tggcattttg ccttcgccga gatgcggttc
    66601 gctgatgtcg tcaggaatat gcaactccac gtagcggctg atgaaattac cccacagcgg
    66661 cccggcaata atcattcccg gaattgccgc acacaggcca atcaggatca tccagccaaa
    66721 atcggcgttc atctgcgatg ccagcagcat tggcgctggc cctggcacca ggaacgccgc
    66781 cgctgccgcc acgcctgcga ataatgggat taccagcttc accaggttcg taccggtgtg
    66841 gcgcgccatt gagaaagcaa cgctaatcag cagaacaatc gccacttcaa agaacagcgg
    66901 tagcgcacag accagccccg caaggccgat ggcataatgc gcgcggctgt gaccgaagga
    66961 tttaagcatt ttgacggcaa tctgatcgac tgcgccggtt tcatgtaaga tcttgccaaa
    67021 catagctccc agggcgacaa ccaccgccag gaagccgagg gtgcctccca tccctttttc
    67081 catcgtcgct gcgattttat cgagcggcat accggaaaaa aggccagccc ccatggacac
    67141 caccatcaaa gccaggaaag cgtgcatacg cgccttcatg actaaaaaca gcagcagtaa
    67201 aacagaccct actgctgtta aaacaagcgt taatgtagtc actacttatt tgcctttttt
    67261 aataacctca atggtgcttg ccacaacacc ttccagcggt tgatcgatat ccaccaccag
    67321 tacatcagtt tcgtccgcac ccggctcctg cagcgtttca aactgcgtca ccaacatttg
    67381 ggttttaaag aaatggcctt tgcgcgcttt caggcggctt tcaatcacgt caaaatcgcc
    67441 tttcaggtag atgaaagaga gattcggatt accttcacgc agcaagtcgc gatagtgttt
    67501 tttcaatgca gaacagacga tcagtgacac tttattagtg cgctgcatag caaacgcggc
    67561 gtcgttcagc gcctgcaacc acggtttgcg atcgtcgtca ttcagcggtt cgccagacgc
    67621 cattttttcg atattgcgcc gtggatggag gaaatcgcca tcaagaaacg cggcatgaag
    67681 ttgatgcgcc acttcactgg cgaccgcaga tttgccgctg cccgatacgc ccatcaagac
    67741 gtaaatgtgg tgatcatggt tagtcgtgct caaagtggtg cccccacaat acaagaatta
    67801 aaaatgttac gggtaactgt tatcggtaac attgtccagc cggacaatat cagaagcaat
    67861 atccatccgt gccttaagtg tataagtgtg agctacttca aatttgtagg cttaaataga
    67921 tccgcccggt gacaaggtga aacctaaatc taacattttc ggtgtcacag attcgccacg
    67981 aatacgcgcc agcaggcgtt cagcgccaat actgcccatc cgctcacgcg gcgtcagcac
    68041 gctcgctaac cgcggctcca tcacctggcc aatgtcatga ccgtggaaac cggcaatcgc
    68101 catatcgtca ggaaccttca atcccagacg ctgacattca aacgccgcgc cgaccgccag
    68161 gtcatcgttg gtacagaaca cgccatccag ctgcggatat tcccgccgcg cctggcgaat
    68221 cagttcaata ccggaagagt aagaagaaga ttgctcaacc atcacgctat acggcaccag
    68281 acctgcatcc agcatcgcct gttcgtatcc cttctgtttg atgatagtac gttcgtcgag
    68341 acgcgcgccg agataggcaa tgtggcaatg cccgcgagca ataatggcgg tggtcatctg
    68401 gcgtgccgct tcaaagttat caaaaccgac ggcgatatcg aggcacggcg actggctgtc
    68461 catgagttcc accaccggga tccccgccac ttcaatcatc tttaaggtgc gcggcgtgtg
    68521 ggtacgttcg gtgaggatca gtccgtcgat attccatgag agcatggatt cgagacgttc
    68581 ttgctccatt tccggtttat aaccgtagtg cgccagcatg gtctgataac cgtgcgcgtc
    68641 ggtgacgctt tcgattccgc gtaatacttc cgcgaaaacc tggttggtga gagaaggtaa
    68701 caggacgcca atcgcccggc tggtggcgtt agagaggata tcgggcgcac gattgggaat
    68761 atagcccagt tcatcaagag cggcggcaat cttgccgcgt agagcgacgg aaacctgctc
    68821 cgggttgcgt aaaaaacggc tgaccgtcat tttggtcacg cctacacggt cagccacatc
    68881 ctgaagtacg ggtcttttct ttttcatcgt cctgaaggta caaaagagat agattctctt
    68941 agtttaacac ggacgcatca gaaccttccc ggggaaaaca gtgcttgttt acaattatta
    69001 gatatacact aaatcaattg catcgcattg tgctaacgac gcggcaaaac cccggaagcg
    69061 tgcatcagca cgcgaccgga gcgagcgagg aagcctgcaa aaaggcaggc ttcaaaaatg
    69121 ccgtggagct ttaaaccggc ggcagatcga acagtaacac ttcgctgtcg ctatccgcat
    69181 ggatggagat tgcctgctca tcccagattg ccagaccatc gctggtcgag gctttcacgc
    69241 cgttaatggt gacattgcct ttcaccacct ggatccagac gcggcgttca gcggcaatct
    69301 gatgcaccga ctgctcatct ttcagcaacg cccagcggta cagttccata tcctgatgca
    69361 ctttcaatga accatctcgc gcatccggcg agagcaccag ctgtttgccc tgtacggcat
    69421 cgaagcgacg ctgttcataa cgcggcgtaa taccgttttc ttcaggcatg atccagatct
    69481 gatacagatg cagacgctcg gtgctgcttg ggttgtactc tgagtgacga atacccgtac
    69541 ccgcactcat aatctggaac tcacccgccg gaacctgctc tttattgccc atgctgtcct
    69601 gatgctcaac agtaccttcc agcacgtagg tcaaaatttc catatcttta tgcgggtgag
    69661 tgccgaagcc ctgccctgct tcaatcacgt cgtcgttaat cacgcgcagc gcggagaagc
    69721 ccataaagtt cggatcgtaa tagttggcaa aagagaaagt atgccaggag tccagccagc
    69781 catgatttgc atgaccacgt tcatttgctt tgcgtaagta gatcatttgt attcgccccc
    69841 tgaatgattt cgatgggctt agtgtggacc cgatctgcct gggatgatag agggtgaaaa
    69901 ttgacccctc tgttcaaaaa aattgaacta ttcgaggggc catcaaactt aattcgcgag
    69961 agtcactgtg gagggagagg cttgctcaaa tccgcgttca aggatttcca gattggtaag
    70021 aacttcagat tccttgacgt aatttggcgc accgtgggtg atggtttgat acaacgcatc
    70081 ataaacgcgc ccgtaatcgc ccatctccgg cttcatctct tctctgaccg tcacgccctc
    70141 gtcattgaca tactccagca caccgaccga attatccgct gcgaatcccg gttcgcccgg
    70201 cataatatta gccttcaggc tggtttcctg ctggtcgata ccgtatttaa taaacgaacc
    70261 tttcttaccg tgaacgataa atttcggata atcgattttc accagatggc tggttttgac
    70321 gatggctttc aggtcgccat aaaacagttg cgcttcaaag gtgtcgtcag gattggcttt
    70381 attacgcagg ctgcggatgt cataagcgac gtgatccggg cgaccgaaca gagaaataat
    70441 ctggtccatc gtatgcacac caaggccata aaacgcgcca tcctgcggca gcccaggttt
    70501 ggtttctgcc accgggcggt aatagtcaaa atggctttcc acttcaacaa tctctcccaa
    70561 cttgccactt tcaatcgctt ttttcgctgt caggaagcag gagtcaaagc gacgattctg
    70621 atacggcgtg acggtcagcc ctttgctttt cgccaacgca aacagctctt tcgcctgcgc
    70681 aagtgtcgga gtgaacggtt tttcgaccag cacatttttc ccggcttcca gcgcgcgctt
    70741 cgcgtactcg aagtggctgt ccgcgtgggt gcagacaaca accagcttaa catcgggatc
    70801 gtttagtact tcgtcgagat cgctggtgaa atggatatgg gaataaatgg gagcctgttc
    70861 ttccggcttc gcatggcgac gaaaaatatg cgcgacatgc cagctatcct tgcggttaag
    70921 tacatacggc agatggtaac gggtggtgct tttgccgaag ccaataaagg cgcagttgat
    70981 gaccatgatt tcgtcctttt taaggtggtt atttacacct tagcgcaaag cggacgtggt
    71041 tcctactgga gtgcgcataa cgaacacaag cactcccgtg gataaattga gaacgaaaga
    71101 tcaaaaaaaa agccagcacc cggctggcta agtaatactg gaagcaatgt gagcaatgtc
    71161 gtgctttcag gttctccgcg agggtcttcc tgatcgcgag acaataataa tcattctcat
    71221 tcgcacttgt ccaacacttt ttgcaaaaaa atgcatttga ctcgcatttg aaagtcaatg
    71281 atgttgaaag ggacatttac cccaaagagg acaaaggaat gagtgagata gtaatacgcc
    71341 acgcagaaac acgggattac gaggccatca ggcagattca cgcccagccg gaggtgtatt
    71401 gcaacacact acaggtgcct catccttccg atcatatgtg gcaggagcga ctcgccgatc
    71461 gtcccggcat caagcaactc gtcgcctgta ttgatggaga cgtcgtgggc catctcacca
    71521 ttgacgtgca acagcgccca cgccgcagtc atgttgccga ttttggtatc tgtgtcgact
    71581 ctcgttggaa gaaccgcggc gtcgccagcg ccctgatgcg agagatgatt gaaatgtgcg
    71641 acaactggtt gcgggtagat cgcattgaac taaccgtgtt tgtcgataac gccccggcaa
    71701 ttaaggtcta taaaaaatac ggctttgaaa ttgaagggac tggtaagaag tacgcattgc
    71761 gtaatggtga atatgtcgat gcatattata tggcgcgggt gaagtaagat agtgcccttt
    71821 ttctgagatg gaaaaagggt gtcattcaaa atcgacatac cttcctttaa ggtatttatt
    71881 tgcccaatac atatattgag atttaactca taattacttc ctaaagtgta atattttatt
    71941 ttttaatata tacgcctaca atttcctgga gtaaa
//
LOCUS       DAZGLJ010000003        69635 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000003 DAZGLJ010000000
VERSION     DAZGLJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.003"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..515
                     /locus_tag="ACHK49_000151"
     CDS             <1..515
                     /locus_tag="ACHK49_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO9260855.1"
                     /translation="KFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQETK
                     TVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVGGG
                     AVGAAVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNETRI
                     QPNTKCPEKS"
     gene            531..1070
                     /locus_tag="ACHK49_000152"
     CDS             531..1070
                     /locus_tag="ACHK49_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO9260856.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKRVGKAEENKSDSWFN"
     gene            complement(1165..2742)
                     /gene="guaA"
                     /locus_tag="ACHK49_000153"
     CDS             complement(1165..2742)
                     /gene="guaA"
                     /locus_tag="ACHK49_000153"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO9260857.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2811..4277)
                     /gene="guaB"
                     /locus_tag="ACHK49_000154"
     CDS             complement(2811..4277)
                     /gene="guaB"
                     /locus_tag="ACHK49_000154"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO9260858.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4439..5809
                     /gene="xseA"
                     /locus_tag="ACHK49_000155"
     CDS             4439..5809
                     /gene="xseA"
                     /locus_tag="ACHK49_000155"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO9260859.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQTVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5806..6021)
                     /locus_tag="ACHK49_000156"
     CDS             complement(5806..6021)
                     /locus_tag="ACHK49_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO9260860.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6090..7562)
                     /gene="der"
                     /locus_tag="ACHK49_000157"
     CDS             complement(6090..7562)
                     /gene="der"
                     /locus_tag="ACHK49_000157"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO9260861.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7680..8858)
                     /gene="bamB"
                     /locus_tag="ACHK49_000158"
     CDS             complement(7680..8858)
                     /gene="bamB"
                     /locus_tag="ACHK49_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO9260862.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGLTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8869..9489)
                     /gene="yfgM"
                     /locus_tag="ACHK49_000159"
     CDS             complement(8869..9489)
                     /gene="yfgM"
                     /locus_tag="ACHK49_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO9260863.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9507..10781)
                     /gene="hisS"
                     /locus_tag="ACHK49_000160"
     CDS             complement(9507..10781)
                     /gene="hisS"
                     /locus_tag="ACHK49_000160"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO9260864.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10892..12010)
                     /gene="ispG"
                     /locus_tag="ACHK49_000161"
                     /gene_synonym="gcpE"
     CDS             complement(10892..12010)
                     /gene="ispG"
                     /locus_tag="ACHK49_000161"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO9260865.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12037..13050)
                     /gene="rodZ"
                     /locus_tag="ACHK49_000162"
     CDS             complement(12037..13050)
                     /gene="rodZ"
                     /locus_tag="ACHK49_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO9260866.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13335..14489)
                     /locus_tag="ACHK49_000163"
     CDS             complement(13335..14489)
                     /locus_tag="ACHK49_000163"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO9260867.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14639..15070)
                     /gene="ndk"
                     /locus_tag="ACHK49_000164"
     CDS             complement(14639..15070)
                     /gene="ndk"
                     /locus_tag="ACHK49_000164"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO9260868.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15219..17531)
                     /gene="pbpC"
                     /locus_tag="ACHK49_000165"
     CDS             complement(15219..17531)
                     /gene="pbpC"
                     /locus_tag="ACHK49_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO9260869.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGAWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NANRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     SYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17532..22493)
                     /gene="yfhM"
                     /locus_tag="ACHK49_000166"
     CDS             complement(17532..22493)
                     /gene="yfhM"
                     /locus_tag="ACHK49_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO9260870.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPSETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNHHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRVDASGYP
                     QSAPLPANDVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22700..23545
                     /gene="sseA"
                     /locus_tag="ACHK49_000167"
     CDS             22700..23545
                     /gene="sseA"
                     /locus_tag="ACHK49_000167"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO9260871.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23881..24006
                     /gene="timP"
                     /locus_tag="ACHK49_000168"
     CDS             23881..24006
                     /gene="timP"
                     /locus_tag="ACHK49_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO9260872.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24038..24814)
                     /gene="sseB"
                     /locus_tag="ACHK49_000169"
     CDS             complement(24038..24814)
                     /gene="sseB"
                     /locus_tag="ACHK49_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO9260873.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPGFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGGEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(24956..26239)
                     /gene="pepB"
                     /locus_tag="ACHK49_000170"
     CDS             complement(24956..26239)
                     /gene="pepB"
                     /locus_tag="ACHK49_000170"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO9260874.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26417..26617)
                     /gene="iscX"
                     /locus_tag="ACHK49_000171"
     CDS             complement(26417..26617)
                     /gene="iscX"
                     /locus_tag="ACHK49_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO9260875.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26629..26964)
                     /gene="fdx"
                     /locus_tag="ACHK49_000172"
     CDS             complement(26629..26964)
                     /gene="fdx"
                     /locus_tag="ACHK49_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO9260876.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(26966..28816)
                     /gene="hscA"
                     /locus_tag="ACHK49_000173"
     CDS             complement(26966..28816)
                     /gene="hscA"
                     /locus_tag="ACHK49_000173"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO9260877.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28833..29348)
                     /gene="hscB"
                     /locus_tag="ACHK49_000174"
     CDS             complement(28833..29348)
                     /gene="hscB"
                     /locus_tag="ACHK49_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO9260878.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29444..29767)
                     /gene="iscA"
                     /locus_tag="ACHK49_000175"
     CDS             complement(29444..29767)
                     /gene="iscA"
                     /locus_tag="ACHK49_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO9260879.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29784..30170)
                     /gene="iscU"
                     /locus_tag="ACHK49_000176"
     CDS             complement(29784..30170)
                     /gene="iscU"
                     /locus_tag="ACHK49_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO9260880.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30198..31412)
                     /gene="iscS"
                     /locus_tag="ACHK49_000177"
     CDS             complement(30198..31412)
                     /gene="iscS"
                     /locus_tag="ACHK49_000177"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO9260881.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31653..32141)
                     /gene="iscR"
                     /locus_tag="ACHK49_000178"
     CDS             complement(31653..32141)
                     /gene="iscR"
                     /locus_tag="ACHK49_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO9260882.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32442..33179)
                     /gene="trmJ"
                     /locus_tag="ACHK49_000179"
     CDS             complement(32442..33179)
                     /gene="trmJ"
                     /locus_tag="ACHK49_000179"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO9260883.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAAQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33298..34101
                     /gene="suhB"
                     /locus_tag="ACHK49_000180"
     CDS             33298..34101
                     /gene="suhB"
                     /locus_tag="ACHK49_000180"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO9260884.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34246..35100
                     /locus_tag="ACHK49_000181"
     CDS             34246..35100
                     /locus_tag="ACHK49_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9260885.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35291..36571
                     /gene="csiE"
                     /locus_tag="ACHK49_000182"
     CDS             35291..36571
                     /gene="csiE"
                     /locus_tag="ACHK49_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO9260886.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36563..37702)
                     /locus_tag="ACHK49_000183"
     CDS             complement(36563..37702)
                     /locus_tag="ACHK49_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO9260887.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37862..38752)
                     /gene="hcaR"
                     /locus_tag="ACHK49_000184"
     CDS             complement(37862..38752)
                     /gene="hcaR"
                     /locus_tag="ACHK49_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO9260888.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPVYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38888..40249
                     /gene="hcaE"
                     /locus_tag="ACHK49_000185"
     CDS             38888..40249
                     /gene="hcaE"
                     /locus_tag="ACHK49_000185"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO9260889.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40246..40764
                     /gene="hcaF"
                     /locus_tag="ACHK49_000186"
     CDS             40246..40764
                     /gene="hcaF"
                     /locus_tag="ACHK49_000186"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO9260890.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40764..41084
                     /locus_tag="ACHK49_000187"
     CDS             40764..41084
                     /locus_tag="ACHK49_000187"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO9260891.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41081..41893
                     /gene="hcaB"
                     /locus_tag="ACHK49_000188"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41081..41893
                     /gene="hcaB"
                     /locus_tag="ACHK49_000188"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO9260892.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41903..43105
                     /gene="hcaD"
                     /locus_tag="ACHK49_000189"
     CDS             41903..43105
                     /gene="hcaD"
                     /locus_tag="ACHK49_000189"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO9260893.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43202..43624
                     /locus_tag="ACHK49_000190"
     CDS             43202..43624
                     /locus_tag="ACHK49_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO9260894.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43672..44544)
                     /locus_tag="ACHK49_000191"
     CDS             complement(43672..44544)
                     /locus_tag="ACHK49_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO9260895.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44556..45650)
                     /locus_tag="ACHK49_000192"
     CDS             complement(44556..45650)
                     /locus_tag="ACHK49_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO9260896.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45683..46681)
                     /locus_tag="ACHK49_000193"
     CDS             complement(45683..46681)
                     /locus_tag="ACHK49_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9260897.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46706..48217)
                     /locus_tag="ACHK49_000194"
     CDS             complement(46706..48217)
                     /locus_tag="ACHK49_000194"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO9260898.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIIAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            complement(48240..49223)
                     /locus_tag="ACHK49_000195"
     CDS             complement(48240..49223)
                     /locus_tag="ACHK49_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO9260899.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGGDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49320..52601)
                     /locus_tag="ACHK49_000196"
     CDS             complement(49320..52601)
                     /locus_tag="ACHK49_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO9260900.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNQEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52719..53912
                     /locus_tag="ACHK49_000197"
     CDS             52719..53912
                     /locus_tag="ACHK49_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO9260901.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVSTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(53976..55229)
                     /gene="glyA"
                     /locus_tag="ACHK49_000198"
     CDS             complement(53976..55229)
                     /gene="glyA"
                     /locus_tag="ACHK49_000198"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO9260902.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55557..56747
                     /gene="hmpA"
                     /locus_tag="ACHK49_000199"
     CDS             55557..56747
                     /gene="hmpA"
                     /locus_tag="ACHK49_000199"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO9260903.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56792..57130)
                     /gene="glnB"
                     /locus_tag="ACHK49_000200"
     CDS             complement(56792..57130)
                     /gene="glnB"
                     /locus_tag="ACHK49_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO9260904.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57191..58525)
                     /gene="glrR"
                     /locus_tag="ACHK49_000201"
     CDS             complement(57191..58525)
                     /gene="glrR"
                     /locus_tag="ACHK49_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO9260905.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58515..59228)
                     /gene="qseG"
                     /locus_tag="ACHK49_000202"
     CDS             complement(58515..59228)
                     /gene="qseG"
                     /locus_tag="ACHK49_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO9260906.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(59393..60820)
                     /gene="qseE"
                     /locus_tag="ACHK49_000203"
     CDS             complement(59393..60820)
                     /gene="qseE"
                     /locus_tag="ACHK49_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO9260907.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCICRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61396..65283)
                     /gene="purL"
                     /locus_tag="ACHK49_000204"
                     /gene_synonym="purI"
     CDS             complement(61396..65283)
                     /gene="purL"
                     /locus_tag="ACHK49_000204"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO9260908.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGLGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIGSLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65541..67097
                     /gene="mltF"
                     /locus_tag="ACHK49_000205"
     CDS             65541..67097
                     /gene="mltF"
                     /locus_tag="ACHK49_000205"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO9260909.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(67094..67597)
                     /gene="tadA"
                     /locus_tag="ACHK49_000206"
     CDS             complement(67094..67597)
                     /gene="tadA"
                     /locus_tag="ACHK49_000206"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO9260910.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(67655..68290)
                     /gene="yfhb"
                     /locus_tag="ACHK49_000207"
     CDS             complement(67655..68290)
                     /gene="yfhb"
                     /locus_tag="ACHK49_000207"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO9260911.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68499..69347
                     /locus_tag="ACHK49_000208"
     CDS             68499..69347
                     /locus_tag="ACHK49_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO9260912.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            69403..>69635
                     /gene="yfhL"
                     /locus_tag="ACHK49_000209"
     CDS             69403..>69635
                     /gene="yfhL"
                     /locus_tag="ACHK49_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO9260913.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFV"
BASE COUNT        16505 a        19230 c        17583 g        16317 t
ORIGIN      
        1 tgaaatttaa aaaatgtctt ctgcctgtgg caatgttagc gtcattcact ctggcaggat
       61 gccagtcaaa tgctgacgat catgccgccg atgtttatca aaccgatcaa ctgaatacca
      121 aacaagaaac taaaaccgtt aatattattt ccattcttcc cgcaaaagtt gccgtagaca
      181 actcccaaaa taaacggaac gcacaagcct tcggcgcgct tattggcgca gtcgctggcg
      241 gtgttatcgg ccacaacgtc ggttctggca gcaattccgg aacgacggca ggggcagttg
      301 gcggcggagc tgtaggcgcg gcagtgggtt ctatggtgaa tgataaaacc ttagtggaag
      361 gtgtttcttt aacatataag gaaggcacca aagtgtatac ctctacccag gtgggtaaag
      421 agtgccagtt tacgacaggt ttagccgttg ttattaccac aacgtataac gaaacgcgta
      481 ttcagccaaa tactaaatgt cctgaaaaga gctaataatc aggaggagtc atgaagaaag
      541 tttttctttg cgccatcttg gcctccttaa gctatccggc tatcgcctca tcattgcagg
      601 atcaactctc tgctgtagca gaagctgaac agcaaggtaa aaatgaagag caaaggcagc
      661 atgacgaatg ggtcgcggag cgcaacaggg aaatccagca agagaagcaa cgtcgcgcaa
      721 atgcccaggc cgccgctaac aaaagagcgg caacggcagc ggcaaataag aaagctcgtc
      781 aggataaact ggacgccgaa gcctctgcgg acaaaaaacg cgatcaaagt tatgaagatg
      841 agctacgcag cttagagatt cagaaacaaa aactggcgct ggcaaaagaa gaagcccgcg
      901 ttaagcgaga aaacgaattt atcgatcagg aactgaagca caaagctgcg caaaccgatg
      961 tggtgcaatc tgaagctgac gccaacagaa atatgactga aggcggtcgc gatctgatga
     1021 aacgcgtggg caaagcagaa gagaacaaat cggacagctg gtttaattaa tcgatgttag
     1081 taacttcaag cctatgattc ttgaagataa aaaaccctct gtagtaacag agggttttgt
     1141 tcattcatag tgcagggatt aaaatcattc ccactcaatg gtagctggcg gcttgccgct
     1201 gatgtcatac accacgcggg aaataccgtt cacttcattg ataatgcggt tggaaacgcg
     1261 accgaggaaa tcgtacggca gatgcgccca gtgtgcggtc ataaagtcga tggtttcgac
     1321 agcacgcaga gagacaaccc aatcatactt acgaccatcg cccattacgc caacggaacg
     1381 taccggcagg aacacagtga acgcctggct gactttgtcg tacaggtccg ctttacgcag
     1441 ttcttcaatg aagatggcgt cagcacggcg cagcaggtca cagtactctt tcttcacttc
     1501 acccagcaca cgaacgccaa ggcctggtcc cgggaacggg tgacggtaca gcatgtcgta
     1561 cggcaggccc agctccagac caatcttacg cacttcgtct ttgaacagct ctttcagcgg
     1621 ttcaaccagg cccatcttca tctctttcgg caggccgccc acgttgtggt gagatttgat
     1681 gacgtgtgct ttaccggttg cagaagccgc agattcgata acgtcagggt agatggtgcc
     1741 ctgcgccagc cacttcacgt cttccagttt cagcgcttct tcatcgaata cttcaacgaa
     1801 aacgcgcccg atgattttac gttttgcttc cggatcgttt tcgccagcca gcgctgacag
     1861 gaagcgatct tctgccggta cgtgaacaat gttaagacca aagtgatcgc caaacatatc
     1921 cagaacctgc tctgcttcgt tgagacgcag caggccgttg tcgacgaata cgcaagtcag
     1981 gtttttaccg atagcgcggt gcagcagcat tgcggttacg gaggaatcca caccaccaga
     2041 gaggccgagg atgactttat cgtcgcctac ctgctcgcgg atgcgagcta cagcatcgtc
     2101 gataattttc gctggcgtcc acagggcttc acactggcag atatcacgca caaaacgctc
     2161 cagcatgcgc ataccctggc gggtgtgagt cacttccggg tggaactgta cgccatagaa
     2221 gcgtttttct tcgttagcca taatggcaaa cgggcagctt tcggtgctgg ctacggtgac
     2281 gaagtcggac gggatagcgg taactttatc gccgtggctc atccagacat cgagcagcgg
     2341 tttaccgtct gcggtcagcg catcttcgat accgcgaacc agtgcgctgt cgtttacgac
     2401 ttcaacctgc gcgtagccaa attcacgttc gttagaggct tcaacgtgac cgcccaactg
     2461 cattgccatg gtctgcatgc catagcaaac gccgaatacc ggtacgcctg cttcaaagac
     2521 atactgcggc gcacgcgggc tgttttcttc ggtggtgctt tccgggccgc cggaaagaat
     2581 aatgccgctt ggattgaagt cacgaatttg tgcttctgtc acatcccacg cccacagttc
     2641 gcagtaaaca cccagctcac gcacgcggcg cgcaaccagt tgagtgtact gagaaccgaa
     2701 gtccagaatg aggatgcgat gcttatgaat gttttccgtc attgacgctt attccgaggc
     2761 aagtgaaaca gataatataa atcgcccgac atgaagtcgg gcgaagagaa tcaggagccc
     2821 agacggtagt tcggggactc tttagtaatg gtcacgtcgt gaacgtggct ttcctgaatg
     2881 cccgcaccgc tgatacgtac aaactccgct ttagtacgca gttcgtcgat agtaccacag
     2941 ccggtcagac ccatacagga gcgcaggccg cccatctgct ggtgaatgat ctctttcagg
     3001 cgacctttat aagctacgcg accttcgata ccttccggca ccagtttgtc ggcagcgtta
     3061 tcgctctgga aataacggtc agaggaacct ttggacatcg cgcccaggga acccatacca
     3121 cggtaagatt tgtaagaacg gccctggtag agttcgattt cacccggaga ttcttcagta
     3181 cccgccagca tggaacctac catcaccgcg cttgcgccag ctgcgatagc tttggcgatg
     3241 tcgccggaga agcgaatacc accatctgcg ataaccggaa taccggtgcc ttccagggct
     3301 tcaactgcgt cagcaacggc agtgatctgc ggaacaccta cgccagtaac gatacgagtc
     3361 gtacagatag aaccagggcc gataccgact ttaaccgcgc tgcaaccagc ttctgccaaa
     3421 gcgcgtgcac ctgcagctgt tgccacgttg ccgccgatga tttgcagatc cggatattta
     3481 gcacgggttt cacggatacg ctgcaaaacg ccttctgagt gaccgtggga ggagtcgatc
     3541 agcagaacgt caacgccagc ggcaaccagc gcgtcaacac gctcttcgtt acccgcacct
     3601 gcgccaactg cagcaccaac acgcagacgg ccttgctcgt ctttacaggc gttcggttta
     3661 cgttccgctt tctggaagtc tttcacggtg atcatgccga tcaggtggaa ttcgtcatca
     3721 accaccagcg ctttttcaac gcgtttttcg tgcatttttg ccagcaccac ttcacgggct
     3781 tcaccttcac gcacggtgac cagacgctct ttcggcgtca tgtaaacgct aaccggctgg
     3841 ttcaggtcgg taacaaaacg cacgtcacga ccggtgataa tacccaccag ttcgttttct
     3901 tcggtaacga ccggatagcc cgcaaaaccg ttacgctcgg tcagttcttt cacttcgcgc
     3961 agcgtcgtgg ttggcagaac agtctgcgga tcagtcacca caccagattc gtgttttttc
     4021 acacgacgaa cttcttctgc ctggcgttca atggacatgt ttttgtggat aaagccgata
     4081 ccgccttcct gagccagagc aatagccagg cgcgcttccg ttacggtatc cattgctgcg
     4141 gaaagcatag ggatattcag acgaatagtt ttcgtcagct gggtgctgag gtcagcagta
     4201 ttcggcagaa cggtagaatg agcaggaacg aggagaacgt cgtcaaacgt cagagcttct
     4261 ttagcgatac gtagcatggg caatatctcg accagagtgg ttaataaata ttgccgcggc
     4321 attatacaga gcgtaaccga ttgcatctac ccctttttgc aaaaaatgct tgctatcccc
     4381 gaagggcggg ttactatcga ctgaataacc tgctgattta gaatttgatc tcgctcacat
     4441 gttaccttct caatcccctg caatttttac cgttagtcgc ctgaatcaaa cggttcgtct
     4501 gctgcttgag catgagatgg gacaggtttg gatcagcggc gaaatctcta atttcacaca
     4561 accggcttcc ggtcactggt actttacact caaagacgac accgcccagg tacgctgcgc
     4621 gatgttccgc aacagcaacc gtcgggtgac cttccgtcca cagcatgggc aacaagtttt
     4681 agttcgcgcc aatattacgc tctacgagcc gcgcggcgac taccagataa tcgttgagag
     4741 tatgcagccg gccggtgaag ggctgctgca acagaagtac gaacagctca aagcgaagtt
     4801 gcaggctgaa ggattgttcg atctgcaata caaaaaacca cttccctccc ctgcgcattg
     4861 cgttggtgtg atcacctcaa aaaccggtgc tgcgctacat gatattttgc atgtgttaaa
     4921 acgtcgcgat ccttctctac cggtgatcat ctaccccacc gccgttcagg gcgatgacgc
     4981 gccggggcaa actgttcgcg ccattgaact ggcgaatcag cgcaatgagt gcgacgtgtt
     5041 gatcgttggg cgcggcggcg gttcgctgga agatttatgg agttttaacg acgaacgcgt
     5101 agcgcgggcg atttttgcca gccgcattcc ggtcgtcagc gccgtcgggc atgagacgga
     5161 tgtgaccatt gccgattttg ttgccgatct gcgtgcgcca acgccgtctg ccgccgctga
     5221 agtagtgagc cgtaatcagc aagagttact gcgccaggtg caatcgaccc gtcaacggct
     5281 ggagatggcg atggattatt atctcgccaa ccgcacgcgt cgctttacgc agatccatca
     5341 ccgattacag caacagcatc cgcagctccg gctggcacgc cagcaaacca tgcttgagcg
     5401 cctgcaaaag cggatgagct ttgcgctgga aaatcaactt aagcgtaccg ggcaacagca
     5461 gcagcggtta acacagcggc tgaatcagca aaatccacag ccgaagattc atcgcgcgca
     5521 aacgcgcatt cagcaactgg aatatcgttt agcagaaacc ctgcgcgtac agcttagcgc
     5581 cacgcgtgaa cgtttcggta atgcagtaac gcacctcgaa gccgtaagcc cactgtcaac
     5641 gctggcgcgt ggatacagcg ttactactgc tactgacggc aatgtactga aaaaagtgaa
     5701 gcaagttaaa gcgggtgaaa tgctaaccac acgtctggaa gacggctgga tagaaagtga
     5761 agtaaaaaac atccagccgg taaaaaaatc gcgtaaaaag gtgcattaag ccagcacgaa
     5821 ttcaacgcgt tttttcgata tcaagccatg cccgtgctga cagaagtaat ctactgcacc
     5881 gcaggctttt aacacctgca acggttgatg gcagtctgga caaagagctt tcatttcaat
     5941 aatttccccg caggatgggc aacgcgcatg gtcgttatcc tgatcaagca catgctgaca
     6001 ttgtggacaa tgcagttcca tatgaattcc tccggtagcc atccctctac attcatagag
     6061 ggaatggcag ataaaatact tacggataat tatttatttt tcttgatgtg cttcatcaga
     6121 cgcttacgtt tacgcatctg ggttggcgtc agggtgttac gcttgttcgc atacgggttt
     6181 tccccttctt tgaactgaat acgaatcggc gatcccatta cgtccagcga tttgcggaag
     6241 tagttcatca agtagcgctt gtaggaatca ggcaggtctt tcacctgatt accgtgaatc
     6301 accacaatcg gcgggttata accaccagcg tgggcatatt tcagcttcac acgacgaccg
     6361 cgtaccagcg gcggttggtg atcttcaaca gccatcgtca tgatgcgcgt cagcatagag
     6421 gtccccacac gacgggtgga gctgtcatac gcttcacgta ctgattcaaa caagttacca
     6481 acaccactgc cgtgcaaggc agagataaag tgcacacgag caaaatcgat aaagcccaga
     6541 cggaagtcca gcgtttcttt cacctgctct ttcacttcct ggctcaggcc atcccactta
     6601 ttcaccacaa tgacaagtga gcgcccacta ttgagaataa agcccagcag cgagagatcc
     6661 tgatcggaaa taccttcgcg cgcatcaatc actaacatca ccacgttggc atcttcaatg
     6721 gcctgcaacg ttttgattac ggagaatttc tctacagcat cggtgatttt gccgcgttta
     6781 cgtacgccag cggtgtcaat gagcacatac tcacgtccat cgcgttccat tggaatgtag
     6841 atactgtcac gcgtcgtgcc aggcatgtca taaacaacaa cgcgctcttc accaagaata
     6901 cggttagtga gtgtagactt acctacgttc ggacgaccca caatcgccag tttgatcggc
     6961 agactttgcg ggtcgaagtc gtcttcctct tcttcttcgc cgttctcttc cgcttcaaat
     7021 tgcgcccagt attcagcgtc ttcgtcgact tcctcttgcg gtgcgagatc ttccatccac
     7081 ggcagcagca catgctccag cagacttaat acgccacgac cgtgagacgc ggcgatcggg
     7141 tagatttcac ctaaaccaag cgagtagaaa tcaaccactg cctgatcggg atccagaccg
     7201 tcagttttgt ttgccaccag gaaggtcggt ttttcacggg agcgcagatg tttggcaatc
     7261 gcttcatctg ccggcatcag gcccgcgcgc gcatccacca taaacagtac gacgtccgct
     7321 tcttcaatcg ccagcagcga ctgttccgcc atgcgggttt ctacaccgtc ttctgtgcca
     7381 tcaatcccgc cggtatcaat acagataaac tcacggcctt caatttccgc acgaccgtac
     7441 ttacggtcac gagtcagacc cgggaaatcc gcaaccagcg catctcgggt gcgagttaga
     7501 cggttaaata acgtggattt tcctacgtta gggcgcccga caagcgcgac cacaggtacc
     7561 atgtttaaag cctcattttt ataaatcatc agacaacgca cgctatattc gcgtcgttgt
     7621 taaaaacagg aaaacggccc ctgtccagga gccgttttca aagtgaacga cagagacgat
     7681 taacgtgtaa tagagtacac ggttccgtct tttgcctgga tcagcagttt gccgtcagcg
     7741 gcaaccggtt cagtctggaa accggaacta tcaacttttt gctgggcaac gaaacgacca
     7801 tcttcgacgt taatccagtg cagataacct tcactgtcac ctaccaccag gttgccatta
     7861 tacagcaccg gagaagtcag caggcgatgc aacagatcgc tttgtgtcca cagcgtaacg
     7921 ccgccatcaa tggtcaacgc catcacccgg tcattttgat cgaccagata gatgcgattg
     7981 ccgtcgacga tgaaatcatt caccgaaccc agttcgcgtt tccacataat ctgaccactg
     8041 cgcagatcaa gcgccgtcag gttaccatta taggccagcg cgaaaacaac gccgttaaca
     8101 acgacgggag tcgtgtcaac atcgctcaga cggtcaattt cggtagaacc ggtcgcctgg
     8161 gaaatacgct gctgccaaat catctggccc tgttccatca gcactgcgct gacgcgacca
     8221 ttatcgccac ccacgacggc cgcaccaaaa gccgttgccg gcgcagactc gccacgcaaa
     8281 gagagcgaag gcatatcgag gttaactgtc catttgacag cgccgtcagc ctcgttcagc
     8341 gcttgtaact gaccgttact ggtgtggatt aacaccagac cgtcgctgac caccgggcgc
     8401 gaaagtgctt cacccgcgac tttagtttgc catgccacag taccatcgct ggtattcagc
     8461 gcgtaaacct gcgccttttc gctgccaatg tagacatgcc caccagacac ggtcagaccg
     8521 ccagaaagta atgcaggctc tttagagaac cagccatctt tctcggccag gctgacagac
     8581 cagatttctt tgccatcatc cgcattcagc gcttttacca aaccagcgcg gtccgctgca
     8641 tagacaacgt tgtccgccag tgccggatga agattggaat agaagttgcc aatgccgcta
     8701 ccaacggaag tgctccacgc cgtggtcggc gtaaactggt tttcaacggt tggcaatggg
     8761 gacatcttta ccacatcttc ttcgctgtta aacagcgaac agccgcttaa aagggtaacg
     8821 gaaagcagtc ctggcagcag taatttacgc aattgcatcg ggtccctctc agatggacaa
     8881 attattaatt ttcatctgca tcatttcgct cagtgccgga gtaacatcgc ttttcacgcc
     8941 tgcttcccat gcactacgcg caccttgctt atcacctttg ctcagcaatg cttcaccacg
     9001 caggtcggca acaatggcag cccacccttc acctttgatg gtatcaaggg ttttcagcgc
     9061 ggcatcagcc tgcttgagct gtacctgaac gcgagcaaga cgcagattta tcacggcttt
     9121 gagattttca tcgctcgtgt ctgccagccc ctgttgtaac tgggcggcag ctttctctag
     9181 ttcatttttg tcaacaaatt gctgcgcaag ttccaaagaa gccagcgcac cataagtatt
     9241 tttattttca gcagcaaatt tttccgccgc cgggatgcta tccggtttgc cttcgctcac
     9301 tgcggtaacc gcattttgat aggcaagaga agcggagcgt gcagaatcaa cctgatggct
     9361 gttccagtag cgccagccaa tcagtgcgcc aacgcccaaa atcaccccaa cagccagtgc
     9421 tttgccattt tcagcaaaaa agcgtttaac cgcttctacc tggtcgtttt cgttctcgta
     9481 aatttccacg ctgtccttct ccttccttaa cccagtaacg tgcgcaaatg cgcggctacg
     9541 ctatcctgcg caactgccgt ttgctcacca gagcgcaaat ccttcactac tgctgtgccg
     9601 ttagccactt cagactcacc cagcaccaca gcaacgcggg caccccattt atcagcacgg
     9661 gcaaactgtt tcttaaagtt gccgccgccg tggttggtca tcaatttcac gcccggtaat
     9721 tcatcacgca gacgctcagc taatgccata gccgcagatt gtgtatcagc acctgaagcc
     9781 accaggtata tatcgacaac aggatcggct ttaaattccg gattaacggc ctgtactaac
     9841 aatacaagac gttcgaggcc catcgcaaaa ccgacagccg gtgttgcacg accgcccagt
     9901 tgttccacaa gaccgtcata acgaccgcct gcacacacgg tgccctggga gccgagactg
     9961 ttagtcaccc actcgaaaac ggtacggtta tagtaatcca gaccacgcac cagacgctgg
    10021 tttacggtgt aagcgatccc cgcgctctca agcagtttgc acagaccggc aaaatgctca
    10081 cgagattcct cgtccagata atcacctaat gccggagcgt cgttgagaag cgcctgcact
    10141 tccggatttt ttgaatccag cacgcgcagc gggttagtgt acatgcggcg tttgcagtct
    10201 tcgtccagct tttctttatg ctgctcaagg aatgccacca gcgcatcgcg gtaattggcg
    10261 cgtgcttcca gcgaaccgat agaattcagc tcaagagtta cgtgttcgga aatacccagc
    10321 gcgcgccacc agcgggcggt gagcataatc agttcagcgt cgatatccgg accttgcaga
    10381 ccgaaaactt cgcagcccaa ctgatggaac tgacgataac gccctttctg cggacgctcg
    10441 tgacggaaca tcggcccgat ataccacaga cgctgttcct gattgtacag aagaccatgc
    10501 tcgatgccgg cgcgtacaca gcccgccgtc ccttcagggc gcagagtcag gctgtcgcca
    10561 ttgcgatcct caaaggtgta catctctttt tcaaccacgt cggtgacttc accaatcgca
    10621 cgtttgaata gcggggtctg ctctacaatc ggcaagcgga tttcactgta accgtagctg
    10681 ccgagcacgt ttttcagtgt gccttcaatg cgctgccaga tggccgtttc gccaggcagg
    10741 taatcgttca tgccgcgaat ggcttgaatg ttttttgcca cgtttattct ctttctgaat
    10801 ataaaaatga accctcaacg cttccctcaa tgtttcggga gctatgcggg ttcaatcata
    10861 cacgggaagc gaggcgcttc ccatcacgtt attatttttc aacctgctga acgtcaattc
    10921 gacgcgcttc gtccagctga ctggctttcg cacgaatgcg tgcttccagc tggtcgatca
    10981 tatcgttgtt gtccagacgg tctttgcgca cgccatcttc atagaggccg cttttcttgt
    11041 tgccgccggt gacgccgagt gtagaaacca gcgcctcacc tgggccattc accacgcagc
    11101 cgataatcga aacgtccatc ggagtgatga tatcttccag gcgttgctcc agcgcgttaa
    11161 ccgtaccgat aacatcaaat tcctgacgcg aacaggtcgg gcaggcgatg aagttgatcc
    11221 cgcgcgaacg gatacgcagc gatttcaaaa tatcgaaacc gactttgatc tcttcgaccg
    11281 gatcggccgc cagtgatacg cgcagcgtgt cgccgatgcc ttcagacagc agcagaccta
    11341 aaccaatggc ggattttact gccccgctgc gtgcaccacc ggcttcggtg atccccagat
    11401 gcagcggctg atcgatctgt tttgccagca aacgataaga ctcaacagcg aggaagacgt
    11461 cagacgcttt cacgctgact ttgaactgat cgaagttcag gcgatcgaga tgatcaacat
    11521 ggcgcatggc ggattccagc aacgcctgcg gcgtcggttc gccatacttt tcttgcagat
    11581 ctttttccag cgatccggcg ttaacgccaa tacggatcgg aatgttttta tcgcgcgcac
    11641 agtcaaccac catgcgaata cgctcttcat taccgatatt gccagggtta atacgcagac
    11701 aatcgacgcc gtattccgct actttcagtg caatgcgata gtcgaagtgg atgtcagcca
    11761 ccagcggcac gttaacctgc tgtttgatga gtttgaacgc ttctgccgcg tccatcgtcg
    11821 gtacggagac acggacgata tcagcgccaa cgcgttccag cgccttgatt tgattgaccg
    11881 ttgcttcgac gtctgtcgta cgcgtattgg tcatggactg tacggcgatg ggagcaccat
    11941 cgccaatcgg cacattccca acgtaaatac gtgttgattt tctacgttga attggagcct
    12001 ggttatgcat gaaaaatctc ccgcgttacc cgtctgttac tgcgccggtg attgttcggc
    12061 attgagggtc agacgcgcaa cctggttagt tctgataaaa cgactcagat cgacaggttt
    12121 cccttgatac tggatctgta ctgcggctgg cgcaccaatt ttcagtttgt atggtgcctg
    12181 gccggttaag ttcaaattac cgtctttacg ctgcataccg ctaaacaatt ttttaccggt
    12241 agcatcagta acctccagcc agcaatcggc agtaaagttc atcaccagcg cattcggatc
    12301 agccgccggc gtagtcacgc ctgcctgatc ggttggcaac ggcgcagcgc catctggcgt
    12361 tgttgttgcc gttggtgccg gggtcgcggc ggtatcaaca tttgcctgcg aaggcgaaac
    12421 aaccgcattc tgttgcggat caacagctgg tgctggcgca gttacggcag gtgtttgcgt
    12481 gttggttgcg gtagtatcca cagacgctgg cggcgtggac gttgtagccg ggtctgtagt
    12541 tgtcgacgta tttaacggaa cactctgccc ctgctcgcta ttactgctca gttccgccga
    12601 agattgatcg gccatagtgg tgatctcttc ctgctgagct ttgtggtctt gccaccacca
    12661 ggcaccgctc aggccgataa ccacaaacaa caccagccag gtgaaggtca tcagccagcc
    12721 gtcacgtttt ttgcggcgtt taccgaggga aaaactctgc ataggcgcaa cttttgcagc
    12781 ccgtagtgga gcctgctttt ccagccctgg cagcagttct tcttctggaa tatgtaccag
    12841 acgcgcataa gagcggatat atccgcgcag gaatgttgaa gcaagatcgg cgggtgcctt
    12901 atcttcttca atgtcgcgta ccgtggaaac cttcaggcaa agtcgctcgg caacggcctg
    12961 ctgactaagt cctagttgtt cgcgagcatt acgcaggcga gcgccggtag taagtgcttc
    13021 attttggtcg tgcgtggctt cagtattcat tcgctgcagg tacgtttaaa tgagaattga
    13081 gatgccggtg aaccatcatg ctcacccaca ccgcgaaaca tccgttaagt taaccgttat
    13141 catacagtat aagaccgtct gtgtgctcgt gacaaagccc acattcacta aggctaactt
    13201 actgttgcat cgttacatac tgccttaaag tcagcaaaaa cgcaccgtta atattgacca
    13261 gacaattgca acttaatact tcattcataa aagattacgc cacggtacat aaagtaaccg
    13321 tggcgtaatg gctatcagac cgctttaatg tcgatggctt caccctgcat ccgtttacgc
    13381 agggtacgtt tcgtacggtc gataacatcg cccgccaact gaccacaggc agcatcgata
    13441 tcatcaccac gggttttacg cacaatagtg gtgaaaccgt agctcatcag cacttttgag
    13501 aaacggtcga tacggctgtt cgagctgcgt ccatacggcg cgcccgggaa ggggttccac
    13561 gggatcaggt tgatcttaca cggcgtatct ttcagcagtt ccgccagttg gtgcgcgtgt
    13621 tcagtgccgt cgttaacgtg gtcaagcatc acatactcaa tagtgacacg gccctgattg
    13681 gcgttggatt tctccagata acggcgcacc gcagcaagga acgtttcgat attgtacttt
    13741 ttgttgatcg gcacaatttc gtcacgaatt tcgtcgttcg gcgcgtgcag ggaaattgcc
    13801 agtgcaacgt cgatcatatc gcccagttta tccagcgccg gaactacacc ggaagtagaa
    13861 agcgtgacgc gacgtttaga caggccaaaa ccgaaatcat caagcatgat ttccatcgcc
    13921 ggaacgacgt tgttcaggtt gagcagcggc tcgcccatgc ccatcatcac tacgttagtg
    13981 attgggcgct gaccggtgac ttttgctgcg ccgacgattt tcgccgcacg ccacacctgg
    14041 ccaataattt ccgatacccg caggttgcgg ttaaagccct gctgggcggt ggaacagaat
    14101 ttacactcca gcgcacaccc cacctgcgaa gagacgcaga gcgtggcacg gtcgtcttcc
    14161 gggatataca ccgtttcgac gcgctgatcg ccaacggcga tcgcccattt aatggtgccg
    14221 tcggatgaac gctgttcttc aaccacttcc ggtgcgcgga tttccgccac ctctttcagt
    14281 ttgccgcgca acactttgtt gatgtcggtc atctcatcaa agttgtcgca gcaatagtga
    14341 tacatccact tcatcacctg atcggcgcgg aagggttttt cacctaaatc tttaaaaaac
    14401 tcccgcatct gctgacggtt gagatccagc aggttgattt ttccatcttt cgtggtgacg
    14461 ttttcaggtg tgactaattg ttcagacata tgctattccg gcctcgttat tacacgttat
    14521 ggcccctgga ggattgaaaa aagaaacgcc ccggtgagcg gctgctcgtc cgggggcgct
    14581 gcattgtaca aattctggcg cacggatgcc acgtttgcac gcggcattta cgaaattatt
    14641 aacgggtgcg cgggcacact tcgccttcgc caaagaaata agcgatttcg cgagcggcag
    14701 attcgacgga atcagaaccg tgggtaccgt tttcggtcag gctgtcagcg taatcagcgc
    14761 gcagagtacc agccagtgcg tttgccggat tggtcgcgcc cagcagatcg cggtgacgct
    14821 gaacggcgtt ttcaccttcc agcacggaaa ccacgatcgg gccagaggtc atgaattcaa
    14881 ccagaccatc aaagaacggt tttccatcgt gttcagcata aaagccacgt gcctgttcaa
    14941 cggtcaggtg cagcattttg gtgccgacaa ttttgaaccc tgcagcttca aagcgcgcaa
    15001 agatattacc aatgacgttt tttgctaccg cgttcggttt gatgatggaa aaagtacgtt
    15061 caatagccat ttttacctct gtaaattgtt ctgttgttgt ctgtaccagc gtacgagatg
    15121 gcgcggatta taatgagcaa cagggccgtt gactattgat gaaggtaaca tttttttaaa
    15181 ataaaatgag ttttagcaac aatcagggac aaaacagact attgcatgac aaatttcact
    15241 gtcgcgattt gccccacatc atccatcacc agcaattgat aatcaccttt atccgtcaaa
    15301 tgcagggtca cgttgcgccc gcgttcagtt aacggttcgc cattcagaaa ccaccagcgt
    15361 tcacctgccc ctccacttga ttgcaacggc aaagtcgctt ccgctgcgcc cggtaaacgt
    15421 ttaataatcg cgccatcgcg aacgcctgtc agctgcagag ggagctgggc atcgtgaccg
    15481 tatgacgggc aaattgtcga ggccggtggt aagcgcacag cgcgacgttc tgatgcgggc
    15541 agccagggtt ccagcggtag cggccagaca tttatcattt cttgtcgcgc ttgcgggcaa
    15601 tcagcggcaa cacgtttgcc attttcatcc agccagatgg ggaaacgaat gccattaatg
    15661 ccttcctgct ccggcagtaa tagagttggc ggctgactcc cgtccagcag ccaggttgcc
    15721 aggcggcggc gacagttacc gtcaccttcc ggcaaagact gtccgcccgg ccagcagata
    15781 acgccacgag tgactgagtt cgggcgcggg tcttccggca gattcgcact gcgcgacagt
    15841 aagatattat tgacctgatt caacaatggt acggcactgg caaagccaaa ctgaccaaca
    15901 acgggcgtgc cgtccggtct gccagtccag atcccaatga catagcgagc gttaacccca
    15961 atcgcccagg cgtcacgata gccatagctg gtgcccgttt tccatgccag tggggcgacg
    16021 cgcggcaagg cactatccgg caagggtttc gcttcatcag ccataatccg gcgaatgatc
    16081 cacgccgccc ccgacgacat taaaggccgt tcaagcagcg gatcgtcagg ctgtaagcgc
    16141 aatttgcctg ccttgccgtg gcgagcaaac gcggtatacg ctgccgccat atcttccagt
    16201 tttgcaccag cgccgccgag aatgagtgaa agattcggcg cagcaccgtt gggcaaatat
    16261 aacggcaatc caacattgcg taactttgcc gcaaaccgtt tcggtccata ggcttccagc
    16321 acctgcacag caggtaagtt cagcgagcgc accagcgcct cgctcatgct gatcgggcca
    16381 tgaaaaccgc tatcaaagtt acctggtcga taatcaccgg tgcgccgggg gacgtcttgc
    16441 agcagtgatg ccgggtggat caagccttca tccagcgcca gaccataaac aaacggtttg
    16501 agcaccgatc ctggcgatcg gttcgcattg accatatcaa catgaccaaa gcgtgaatca
    16561 tcgttgagat caaccgatcc cacccagccg cgaacacgca tatcggtatg atcaaccacg
    16621 atcatcgcca gtgagctgcg cggtggcaac cgccctttcc agttttgcgc cagttcttcc
    16681 agacgtcgtt gaagaccggc atccagcgta gtaacgattt tgtcgctttt gcttttaccg
    16741 agcatcatgc gcgaaaacag cggtgccagt tgcggcattt gtcggggtgc cagccagatg
    16801 ggttcttccc ttgactcttt tacctgctca cgggaccacg caccttgcac ggccatccgt
    16861 tcgagcactt tattacgcgc ggcttcggca cgctccggcc aacgatccgg gcgaagacgg
    16921 ctgggcgctt gcggcaaaac cgccagcatt gccgcctcgg aatagcttaa attcgcaggc
    16981 gactttccga gataagccca acttgccgca ccgatcccct gcaacgtacc gccaaacgga
    17041 gcgcggttaa gatacaaggt cagaatttca cgcttagaca gatgccattc cagttgcaac
    17101 gcgcgccaga gctggcgaat tttgccgcca aatgttttgg ggtgaggatc aagcagacga
    17161 gcaacctgca tagtgagcgt gctgccaccg gaaataaccc gtcccgaagt gagatcttgc
    17221 catgctgcgc gcgccaccga gaatggattc acccccggat gcttccagaa ccagcgatct
    17281 tcataattga tcagcgcttc aaggtaacgt ggagaaacat cttcgattgt taccggataa
    17341 cgccagatgc cgtcagcatc ggcgaagcgc catagcggcg taccatcctg cgccacgacc
    17401 actcgtgcgg gattgacttc atgcagcggt agaggccaga gtttatctgc tccccacgct
    17461 gcaagaaaaa ggagaaatgg cgcagctgcc agcgttatcc agcagccgcg tttggttaac
    17521 aagcgaggca tttacggtcg gacaatcagc agatcttcag ccgcgccggt cgcccgccat
    17581 tggggaacat acattgattc caccatcggt tgcggtacct gatacgttcc cggcgtcacc
    17641 gcccgcgcca gatacaccag cgttaccggt tggtattcat caacggcaac cgccgccaca
    17701 aagcgatcgt cacggaactc aatgtgctta atactcgcct gctgcatctg gttcagtaag
    17761 ttctgcactt cgccaccact ttgctccagg ctggcgctac cgttcgccag attctggttt
    17821 tccagttcca gacccgcagg cagcagatcc acgaccaacg catccggcac gctgttactg
    17881 gcttttacct gcaaccacac cagcaccaga tcgccgctac gtaacgagtc cagcgatttg
    17941 ctcttaccat cagtaccaag aatatgacgc tcgatttgca gcacatcgtt cgccggtaaa
    18001 ggtgcggatt gcggataacc gctggcatcc acacgcagcc ataacggctg atcgccactg
    18061 tttctcacct gcaaggtggc aagttgatcg ctattcagat tgctgtttag cgttttctcg
    18121 cctgtcagcg gctcagctga gaaagaggtt tgcgcctgcc atttacccgg taaatcctga
    18181 atcgtacggg cagccaggaa caacgcgtta ctttcctgcg tcgatagcca gcgttcacca
    18241 aatgcctgct gcgaaagcgt gttcagcaaa gtgttctgct catcgggtag cagtttattt
    18301 tcttccagca aggagagcat taacgcgttg tcgcgcagtg gactaccgta atcacccagc
    18361 catatccgct catcactatt tctcggcgtt ttcagcgcca gcacaatcgc ttcttcacca
    18421 cgcgttgcat cacccatggt tttcagcgca acgccaagtt gcagcagcgg taaaccagaa
    18481 gcggcatctg cacgatgctc ccagatttca cgcagcgcac ccagtggtgc tttttgctga
    18541 cgggccagca ccagcgcagc gtaagactga acggcgaatt tactggcttt gagattatcc
    18601 gcgtacggga tcgacatcat gcccggatct tgtaaatagc gcagcagacg ctcattaccc
    18661 cggttaatgg cgtctgtcgg cacgctgtaa ccctgttcgc ctgcgcggac caggaaatcc
    18721 atcacgtaag ccgtcagcca gtactcttcg tcaccgtttt tatcccacag cgcaaagccg
    18781 ccgttatcac gttgcatttg cagcaaacgg gaaatgccga tatcgaccga tgcacggcgt
    18841 ttctcatcac tgtcgccttt gatgcccaac gcctgcagtt gggctgcgtt ggtataaagt
    18901 gacgggaaca ggccgctggc ggtttgctca agacagccgt acggatacgc ttttaactct
    18961 ttgatataac gtgcaatgtt cagcggtggt ttgccgctca acaacaattg cccttccagc
    19021 gtaacaggcg agaagttttg caatccatcc gccggaatcg cccatgtctc accaggctgt
    19081 aacgccgtac cgtaattaac cgtttgtgcc gggaacgccg gacggacgcc gattttccac
    19141 tgcttatgct gatcggcaac ggtttcaccc ggtaacgcta acccgctaat ggtggcctga
    19201 atttctccat cgccataacc cggcaatgct cgcaccggga taaacagcgt agtacgcaca
    19261 cctggcgcta attcaacggc tgcgggtgaa tcgctgacca gttcaagcaa accactggcg
    19321 gtcagggcaa cgttcagttt ttgcggttta tcggtaagat tagtgatatc cagcgtcaga
    19381 cgcgaggtat cgccactcgc cataaagcgc ggcatgttca gttcagcaat caccggtgcg
    19441 gcaactatca ctttgctttc gttgctaccg aaatcatctg ccgtccaggc ttgcgccatg
    19501 acgcgcaatt caccgttaaa atcgccaatc ggcagtgtaa ccgagccttc gccctgttcg
    19561 ttgagcgtta ccggcagcgc ctgctgcgcg acaatattga cgtgattgac cggcggttta
    19621 ccaccacgtt tcagctcatc accatcgcca ccgaaacgca gagctgccag acgcccctga
    19681 ccttcaataa cctgaccgta aatatcgtaa atgtctgcgc catagcgttt ctgaccaaag
    19741 aacgcctgcc acggatctgg cgtgacgtag tcagtaatat tcagcacacc actatcaacg
    19801 gcagacacca gcacattcac ctgtttaggc ttctcgccat ttttagtgct ggctttaatt
    19861 ttcacggtta atggttgatt gggacgcatt tttgctggtg tttccagcgc cagatcgagg
    19921 cgacggtttt catcgccaag cggcagatgc aacacaccaa ccgcgcgttt tggcgtcgcg
    19981 gagcgagatt tatcgccagg acgtaccacc agcgtactta aatacagatc atgatgattc
    20041 caggttttat cgaccggaat cgtcagatcc agcccttgag cccgaacatc aatctcttgc
    20101 caccacagcg gcccttcact ggactcgacc atcgcataac ctttacccgc cgttggcgcg
    20161 gcgatatgca acttaatggt gtcgccaggg cgataactgg ctttatccag tttcagcgtg
    20221 acacggtcgg gtcgcactgc gccgctaccg tcgctgttgt cctgccagct atagccagcc
    20281 cagaaacgaa cactactgac cgcttcattc ggcgctttga cttccagacg ataagcgccc
    20341 cactctaccg gaaaactgac cttgccggtt tcgtccgctt tcagatccag agtttgttca
    20401 ttttcgatca gatctttttg atcaaactgt gactgccagc cttcatcttc tgaccagttc
    20461 cagtagtaat cgcggcgttc gcgaatcagg cgcacctgca agcccgacac ggcttttttc
    20521 acgccttgcg catcgctata aacgatgtca aaagcggcgt tactgccttc atcaacaatg
    20581 ggctgtttca ccgtgctgtc agtacgataa tcgtaaaccg atttcgaggc gaactgcgga
    20641 cggatccccg gcaatgcatc ggcaggccag atagcctgct cagcgcggcg cgtcaccggg
    20701 cgaccgcccg attccagcaa actaccctgg aaaataacct gtaatgggga atgcgtttcc
    20761 ttccactggc tttctgtaga aacttcgccg cgccctttat catccagcgt caactgaact
    20821 tcatccagcg tgcgggaaag attttcggca gctatatcgc cgaattcaaa accaggtaag
    20881 gctgacacag cttcacgcag tgggcgcagg aaaagttgcc cttgcaaagt attaccatta
    20941 gcaggtgcac catacaggta gtaccccacc acggagaatt tcacttcatc tttcggcgtt
    21001 agcggggttt tctcaccggt cagattcagc gccatgcgct ctggcataaa atcttcgacg
    21061 tggaaatccc acatccgata ctggttatcg cccgtgttag cgcgaatatg ccacataccg
    21121 gttgccgcat tgctatcgag tggccaggta aagtggtaga ggccattctc cggctgacta
    21181 acgacgctcc tgagtacctg cccatcgggt ttaatcacgt ctaacttgat gggttgattg
    21241 ggcaacgctt taccgtctgc atcacgcagc aaaccattga ggattaccgt ttcacttggg
    21301 cgataaagat cgcgtgggcc aaacatgaaa aactgtttgc tatagcctgg cgcgccagca
    21361 atgttaaatt ctgctaagtc cagcgccgga agttttaaat cgagtagcgt tgtctgaccg
    21421 tctttacgcg ccaacagtaa tgccgcgttt ttatcatttt ccagctgcac atgcccctga
    21481 gcgtcactgg ttgcctgagt cagagtctgc cctttctcat ttaataaaga gacttcaatt
    21541 ccttgctggg ccgcgccgtt ttccagactt tgggtaaaga tatccagacg attgtgataa
    21601 cggtgagctg aaacgccgat atcacttaac gtaaacagcg tcgcgggatt actgtaatcg
    21661 taacgtccag cctgattcat cacagccaga tacacgcccg cctgctgaag cggtttgata
    21721 tcgcccagcg gcagcaataa tttttcacgg gtgttacgcg caggattgag atcaaaccgt
    21781 ccggtgtaga ccagatccgc catctgcagc agtttgtctg actgccagtt cgccagcgaa
    21841 ttgcggtatt cccattggct aatgaatgct ggcagagatt ctggcttaac gcggaagaag
    21901 ttaacatcga cattattaac gttgagcgcc attaccggca gcccttcaac gactttgcca
    21961 ggcagcagcg aaccacggct ggcaaaaccg acgctgggtt ggatgtcgcg ggtagttata
    22021 gttttttcgt aatctttact gaaggttgcg ttgttgagtg ctttgacctc cttgccaata
    22081 gtaacgatca aatcacgttt cggttcgagg tggcgtaaac gtaactcttt aagattatct
    22141 gacagctccc aggcaccatc cactttgccg ctttttttat cgacgacatg aataacgcgt
    22201 gagaaatcct gatccgggtc gagagggatg gagaacgtca gcaccagcgt ggctgcacca
    22261 tcaagttgga cttcagagag atccagcaat gtcagcacct taccggcact ctgttgggcc
    22321 agtttttgtc tctccggcac ggagagtttt gctgaactcg cgttttctga agaggccgct
    22381 ttagtaactt cagaaggcgc atcttttttc accgctgttg gcgcgttatc gttgttgtcg
    22441 caccctgcca gcgccagcat tagcatgcag gcggctacgc gtaacttttt catttttcat
    22501 ccctgccaca atggcccgtt agcaacgtcg aataattatt atgcgtgaga atttacgtta
    22561 tgtaattcag tatcacgccc atacaatccg aattttagtg agaattgtgg ctcagacagc
    22621 caaatcagcc tctttcgctt gtcgcttttc actaaacatc cgacaattta gcctaacccc
    22681 ggcaaaaatg gagatgccta tgtccacgac atggtttgta ggagccgact ggctcgccga
    22741 acatattgat gacccggaaa ttcagattat tgatgcccgc atggcgtcgc ctggacagga
    22801 agatcgtaac gttgctcagg agtatctgaa tggacatatt cccggcgcag tgttttttga
    22861 tatcgaagcg ctttctgatc acacttcccc gcttccgcac atgctgccgc gcccggaaac
    22921 gttcgccgtg gcgatgcgtg aattaggcgt taaccaggat aagcacctga ttgtctatga
    22981 cgaaggtaat cttttctcag ccccacgagc atggtggatg ctgcgcacct ttggtgtaga
    23041 gaaagtgtcg attctggggg gtggacttgc aggctggcag cgcgatgatc tgctgttaga
    23101 agaaggtgca gtagagctgc cggaaggaga gtttaacgcc gcgtttaatc ctgaagccgt
    23161 ggtgaaagta accgatgtat tattggcaag ccatgaaaat acggcgcaaa ttattgatgc
    23221 ccgcccggct gcacgtttta acgcagaagt tgatgaacct cgcccaggtt tacgtcgcgg
    23281 acatattccc ggagcactga atgttccgtg gacggaactg gtgcgcgaag gcgaactaaa
    23341 aacgaccgat gaactggatg cgatattttt tggtcgcggc gtcagctacg acaaaccaat
    23401 tatcgtcagc tgcggctctg gtgtaacggc agccgtggtt ttgttagcac tcgcgacgct
    23461 ggatgtgcca aacgtgaaac tgtacgacgg cgcatggagt gaatggggcg cgcgggcaga
    23521 tttaccggtt gagccagtga aataagtatt ttacaggcaa taaaaaaccg ccgaatttgg
    23581 cggtttttta ttgctggtct ggttcgcggc ctttccagca ggttgtatta ccgtagtaat
    23641 gcaagcgcgt ctcagcggag acaatactcg ccagtaactc tctttttgtc aagcaaaaga
    23701 gagttattat tgttctgtta gtgtattatc cactgcggcc ctttccgccg tctcgcaagc
    23761 gggcgctggc ttaaggaaag gatgttccgc gaccgttaga gcaagcgcgt ctcagcgctt
    23821 gctatcgcgg caatatcgcc agtggtgctg tcgtgatgcg gtcttcgcat ggaccgcaca
    23881 atgaagatac ggtgcttctg tatcgtactt attgtttctg gtgcgctgtt ttcccaagta
    23941 aataataacc ggagtctctc cggcgacaat ttactggtgg ttaacaacct tcagagcagc
    24001 aagtaagccc gaatgccgcc ctttagggcg gcatatttta gattatccgg ttctgtttaa
    24061 agtcacgcaa aaaaccaccc cagcgacgtt catagaatgg cgcaatatgt tcggtaataa
    24121 agtggctaat tcctttttcc ccttttttca cctgacaaat atcgattggt tcatcgccag
    24181 gtaatgtatc ggtcgctaca cttcccgtcg cctgaataat ttcttcgata tcaccatcgg
    24241 cttcaatgcc aataagtaaa ttaggctgtg cctcttcgtt ctctttaatt gaacaaataa
    24301 aagcacgctt caccggctta atggttttaa ataaggtggt gagtgaatca atcatttgtg
    24361 ctggcggctc tgcgacttcc gataatatca gcgattcacc gccttccagg atttcctggc
    24421 tgctcagcgg atttccctct ccaccaatca acaaactgat ttcacgcggc ataaattctt
    24481 taccggtcgg cagtttggca ttaaggaaga gcgtttcgcc aagggtcatc tcaaacaatg
    24541 tgcgaacggg cattacgaca aatgcctgtt cgtcttcaac cgcctgttga agcgcttcta
    24601 acgaggtgaa aaaaggaatg acgctggtgc cgtcttcttt ttcccagtgc tgtaaatcaa
    24661 gcgcactatc ttcaaccaca gcctcgccct gcgccgccgt accaggcacc cagacggtgg
    24721 attccagtag agtacggaaa aagcccgggc ggtgcgccgg ttcggttgct gctttttcca
    24781 gcaggtcttc aagttcgttt tttgtttcgg acatatgaac cacaattcat tcaacgttcg
    24841 gggcgcaaat gcctgatgcg ctacgcttat caggcctaca agggattcgc aatttgttga
    24901 atttgcagaa tttgtaggcc ggataaggcg tttacaccgc atccggcata ttagtttacg
    24961 ccgttaacag attagctatc gtgcgcacac caagtcccgt agcgcccgca gaccactgtt
    25021 caaccggcgc tttacggtaa gtcgccgagc agtcgatatg cagccagcct tgctgatagt
    25081 tctcaacaaa gtgcgacagg aagcccgccg ccgtgctcgc gcctgccgga tacgccgcgc
    25141 ttccggtatt gttcagttcg gcaaagttag acggcagctg gctgcggtgg aattccgcca
    25201 gcggcagacg ccagaatggt tcgttctctt gtgaggcact cgccagcaaa cgaccggcaa
    25261 gcgcatcgtc aaaactgaac agcgcgtgat aatcattacc cagcgcagtt ttcgccgccc
    25321 cggtgagggt cgccgcatca atgatcattt ccggtttctg cgcactggca tcaatcagac
    25381 catcggcaag caccagacgc ccttcggcat cagtgttcat cacttcaact tttttaccgt
    25441 tgcgataggt gatgatatcg cccagcttga acgcattgcc gctaatcagg ttatccgcac
    25501 agcagaggaa cagcttcacg cgcttgttca gtccgcgcgt aatggcaaat gccagcgccc
    25561 cggtaaccgt tgccgcgccg cccatgtccg acttcatcga gtccataaac gcagtctgtt
    25621 tgatgctgta gccgccggag tcaaaagtga tacctttacc taccaggcac gcgtacaccg
    25681 gtgcttcttt atcgccagtt gggttgtaat ccagcgccag caataccgga gaacgttctg
    25741 aaccgcgccc gacggtgtgc agccccatat aaccttgctc acgcagatct tcgcctttgg
    25801 tgatccgata agtcacacga tcgcccgcga cgttgctgat cagatcaaca gcacgctgtg
    25861 ccagttgcga tggtcccaat tcttctgccg gtgcgttgat ggtgtcacgc acccagtcga
    25921 tgatcatcag gcggttatcc agttcctggc gctgggcatc gtccagatcc ggccactcca
    25981 ctttacgcgt gcctttcggg gctttgtaac cttgccagaa tgcccagcag cgatccgcat
    26041 cccagccttc accgcttaac tgaacatgct tgatgcccag accgtcaatc ttgcgcgccg
    26101 cacgctggat cagccccaga tcgtctgccc cgttcaaatg cagggtaatg ccgtcattat
    26161 taatgctgta agttgctttt tctccccagc gcgcgtcggc aggttgggta gagagggtaa
    26221 tcttcatcgc ttctgtcatt ttagttatcc ttctttgtaa aagggccgcc agttggcagc
    26281 cccaggattt ttccgctaaa tgattcgcgt tgcaggaagg cggcaagtga gtgaagcccc
    26341 aggagcatag ataactatgt gactggggtg aacgagcgca gccaacgcat ctgcggcgtg
    26401 aagcatgacg cggaaattat tcggcctcgt ccagccagac taacaaaatc gcttcgagga
    26461 ttttctcgtt ggatgcctgc gggtcgtcgt cgaaatcttc cagatcgcaa atccactgat
    26521 gcatatcggt gaatcgaacc gttttcggat caagatcggg atacgcatcg tacagtgctt
    26581 cgccaatttc gcggctatcg gtccatttaa gtcccatact aacctctgtt aatgctcacg
    26641 cgcatggttg atagtgtaac gcgggatttc gaccactaaa tcttcgtcgg tgacgcgcgc
    26701 ctggcagctt aaacggcttt ccggctccag tccccaggct ttgtccagca tgtcgtcttc
    26761 ctgctctgag ctttccggca gcgagtcaaa accttcacga acgatgcagt ggcaggtggt
    26821 gcaagcacag gatttttcac aggcgtgttc aatctcgata ccgttacgca gagctgcgtc
    26881 gagaatggtt tcaccgctat tagcttccag aacagcgcca tcagggcaga gatcctgatg
    26941 aggcaaaata acaatctttg gcatattaaa cctcgtccac ggaatggcct ttcagcgcac
    27001 gacgaaccga ctggtccatg cggcgagcgg cgaaatcctg ggtttgtttg tctacgtttt
    27061 taatcgcttg ttcgatggcg tcaacgtcat cgccctgcgc cacttcactc aggtgagcgg
    27121 cagcatcgtc aatgacctga cgttctgcgg cgcttaacag cgcggcatca gcagccagcg
    27181 cgccgtgcag actttccagc acacgcgccg cttctacttt ttgttctgcc agcattcggg
    27241 cttttacgtc ctgctcggca tagctcattg agtctttgat catcgaagcg atttcgctat
    27301 cggtcagacc gtaagacggt ttgacctgaa tagacgcctc aacgccggtg gatttctcca
    27361 tcgccgtcac gctcaaaaga ccgtcggcat cgacctggaa cgtcacgcga atatgcgcac
    27421 cgccagccgg tagcgccgga ataccacgca gcgcaaaacg cgccagtgag cggcagtcct
    27481 gcaccagttc gcgctcaccc tgcattacat ggatagacat cgccgtctga ccatctttaa
    27541 aggtggtgaa atcctgagcg cgggccaccg gaatagtggt attacgcgga atcactttct
    27601 ccaccaggcc gcccatcgtt tcgaggccca gcgacagtgg gatcacatca agcaacagca
    27661 tttcgctgtc tggcttgtta cccaccagaa tatccgcctg aatcgccgcg ccaatagcga
    27721 cgactttatc cgggtcgatg gaagtcagcg gtggacgacc gaaaaattcg cctacccgtt
    27781 cacgcaccag cggcacgcga gtagaaccgc ccaccatcac cacttccagc acttcgtcag
    27841 cttctacacc cgcgtcttta agcgcgcgac gacaagccag taaggttcgt tttaccagtg
    27901 gcgcgatcag ttcattgaat tgttcacggc tgatttcgcc ctgccagccc gcaacgttaa
    27961 cggtcacgga gtccgcatcg ctcagcgcga ttttggctgc aatggcggca tccagcagtt
    28021 cacgctgaac gcggttatcg ctacgatcag gaatgcccgc ctgctcgcga atgtaatccg
    28081 ccagcagatg gtcgaaatca tcaccaccga gcgcggaatc accgccggtc gccagcactt
    28141 caaacacacc gcgacttaag cgcagaatgg aaatatcaaa cgtcccgccg ccgaggtcat
    28201 aaacggcgat cacgccttcc tgaccggaat ccagcccgta ggcgatagcc gcagcggtcg
    28261 gttcgttaag taagcgcagg acgtgaaggc ccgccagacg cgccgcgtct ttggtgccct
    28321 gacgctgggc atcgtcaaag tacgccggaa cggtgataac tacaccatcc agctcgcctg
    28381 ccagggcttc agttgcccgc gccgccagtg ctttgaggat gtccgcagaa acgcgcaccg
    28441 ggttcagcag ccccgccgcc gtttcaatca tcggcaggcc gttttcgctg gcctggaatt
    28501 gataaggcag atgcggatag cgttgctgga tatcagccag cgagcgcccc atcaggcgtt
    28561 taacagaact aatggtgttg gcggtgtcaa gcgctgcgtt agtacgcgcg tcataaccca
    28621 ccgaatgccc ttgctgttga tagtgaacaa cagatggcag caggtgacgg ccttcatggt
    28681 cggctaacgt ttcggcctga ccgctgcgca ctgtcgccac cagcgagttg gttgtgccca
    28741 ggtcaatacc agccgccaga cgacgctgat gcggcgcagc actcaaacca ggttcactaa
    28801 tttgtaataa ggccatgttt agcttccaga aattaaaaat cgagcagttt ttcttcgagt
    28861 tgttcggcac tgctccgcag tttatcgaga aaacgcagct tacgcacggt atccgccgcc
    28921 gcgtcccacg cctcgttgtc taactgttca accatcaact gatggcgggt atcaaacatc
    28981 tttttcacac gtttgataaa gctttccagc cgcgcttcat ctttcgcctg ttcgatctcg
    29041 tccagctctt cgcgcagctc caactgttcc atcaggaacg cggtgtcgcg cacagtatgc
    29101 tgctcgctgg cgagatcaaa accgtgtaaa gaaagcaaat attccgcgcg cattaacgga
    29161 tgacgcagcg tttgccaggc ctggttaatg gttgcagatt gctgtacggc ggcgagttgt
    29221 tccgcctggc ttccgctggc gaatttatca ggatgatact gacgttgtag atcctgaaaa
    29281 cgcaggctca gcgcctgggt atcgagttga taacgggcag gcaagccaaa gagggtgaag
    29341 taatccataa cattctcagg gctgcgatcc gccgcgataa cggcgaatca ggtaaaacaa
    29401 accccacgcg caggcgacca cggtggggtt atcggtatgc gcatcaaacg tggaagcttt
    29461 cgccgcaacc acactcatct ttaacgttcg ggttggtgaa tttaaaccct tcattcaggc
    29521 cttcttttac gaagtccagc tgcgtaccgt ccagaaattg caggcttttg ccatcgacca
    29581 cgactttcac gcctttgtct tcaaacacga tgtcttccgg cgtcggttcg tcaacaaatt
    29641 ccagtacata agccatacct gaacatccgg aggttctcac gcccagacgc aggccaaacc
    29701 ctttgccgcg gttagccaga aaggtattta ctcgcgctgc tgcactgtcg ctcagtgtaa
    29761 tcgacataac caaacctcaa ctcttatttt gcttcacgtt tgcttttata gtccgcaatg
    29821 gcggctttga tcgcgtcttc tgccagaata gaacagtgaa ttttcaccgg cggcagttca
    29881 agttcttcag caatatcggt atttttgatc gcctgcgctt cgtcgagaga cttccctttc
    29941 acccattcgg tgaccaggga gctggaagcg atagcagaac cgcagccgta agttttaaaa
    30001 cgcgcgtctt caatgatacc ttcatcgttg actttaatct gcaacttcat cacgtcgcca
    30061 caggccggtg cacccaccat gccgctgccg acgttctcgt cgttgttgtc aaaggaaccc
    30121 acgttacgcg gattctcgta atggtcgata actttttcgc tgtaagccat tataaattct
    30181 cctgattccg ataccgatta atgatgagcc cattcgatgc tgttcagatc cacgccctgc
    30241 ttgtacattt cccacagcgg agaaaggtca cgcagacgac cgatggattt acgaactaac
    30301 tcgatggtgt agtcgatctc ttcttcagta gtaaaacgac ctaaagagaa acggatagag
    30361 ctatgtgcca gctcgtcgtt cagccccagc gcgcgcagca cgtaggacgg ttcgaggctt
    30421 gctgacgtac aggcggaacc tgaagaaact gcgaggtctt tcagcgccat aatcagcgac
    30481 tcaccttcaa cgtagttgaa gctgacgttg agaatgttcg gcgcaccgtg ttccaggtca
    30541 ccgttcaggt aaacttcttc gatatctttg atgccgttcc acagacggtt acgcaggccg
    30601 cgcagacgtt ccatctcggt cgccatctct tcttttgcga tgcgataggc ctcgcccatt
    30661 ccgacgatct ggtgaacagg cagagtgccg gaacgcatac cgcgctcgtg accgccgccg
    30721 tgcatttgcg cttcgatgcg tacgcgcggt ttacgacgta catacagcgc accgatacct
    30781 ttcgggccat agattttgtg accggagaaa gacatcaggt caactttcaa ctggctcagg
    30841 tcgataggca gtttgcccac gctctgggtt gcatcaacgt gatagataat gccacgagca
    30901 cggcacattt cgccgatagc cgcgatatcc tgcaccacgc cgatttcgtt atttacgtgc
    30961 atgatggaaa caaggatggt gtcgtcacgc atcgctgctt caagttcttt caggtcgata
    31021 atgccgttac gctgcggtgc caggtaggtg acttcaaaac cttcgcgctc cagctgacgg
    31081 caggtatcca gtaccgcttt gtgttcggtt ttgctggtga tgatgtgctt gccttttttc
    31141 tgataaaagt tggctgcacc tttgatcgcc aggttgtcag attcggttgc accagaggta
    31201 aagacgattt cacgcggatc agcgccgacc agatcggcaa tctgattacg ggcgatatct
    31261 accgcttctt cagcctgcca gccgaaacgg tgagaacgag aggccgggtt accaaaggtt
    31321 ccgtccatcg tcataaactg catcattttc tcggcaacac gcgggtccac cggcgtggtt
    31381 gcggagtagt cgagataaat cggtaatttc attgctctat aaactccgta catcactcaa
    31441 tgcaaggaac caggctaccg gctggatgta cgaccgtgtt tacggagcgc gatacgcccc
    31501 ggcctgattc taatatcttg ttatctttaa cctaactgtt tcgggtttca gcaagttgac
    31561 aaacttacga atctccggga gcttacataa gtaagtgacc ggagtgagta tgtgcagtca
    31621 acgcagatgc aacacgaaac aggacggtta aattaagcgc gtaacttaac gtcgatcgcg
    31681 tcttgtatgc gggtgcgtgg cgcgtcgtga gtatgctgac gaccagacac atccagcact
    31741 tcctggttat taaccagttc gcctaaagta atgttgttga gaaaaccggt gagacggtcg
    31801 ctcaaatcac gccacagcgc gtgggtcagg catttatcgc cgccctggca gccgccttta
    31861 ccctgacaac gggtggcatc tacagattcg tcaacggcgc taatcacttc gccaacggcg
    31921 atgctgctgg catctttgcc taacagataa ccaccgcctg gtccacgtac gctggaaacc
    31981 agaccatttt tacgcagacg ggaaaacagt tgttccagat aagaaaggga aattccctga
    32041 cgttcggaaa tatcagccaa cggtaccggg cccgcttcag agttgagcgc aacgtcaagc
    32101 attgcggtca cggcatagcg ccctttagat gtcagtctca tgtcttactt cacctcaaac
    32161 tcgcccctgc ccggggtttt ttattgtaaa agtgggggta ttgcatagca gggtcaagtc
    32221 tgacattccc gagtaaattg gtcaactatt tacttgactg atttagtcgg gtatttaacc
    32281 ttcagtgcca tttttttatc gtggcgtttg tacctgttgt ccttccagac catgggcggg
    32341 ttgagggcaa ttattcgcat tagcgctggc ctcttcccct caccctaacc ctctccccga
    32401 aggggcgagg ggaccgtccg tgcatttatc tggcttagcc ctcaggcctt attaccttta
    32461 ttctgctgct caatagaagc cagaatcccg cgcaggatat tcaattcctg gctttccggg
    32521 cgcgcacggg taaacagacg gcgcaattta ttcatcacct gccccggatg gttttcacgg
    32581 ataaaaccgg ttgccagcag cgtttgttcc agatgaccat aaaaacgctc cagatcatcg
    32641 accagcggat acggcgtctc ttcatgctcg acctgctcgc cgttttcctg agttgccagc
    32701 caggccatgc gcacttcata ggcaataacc tgagccgcca tcgccaggtt cagcgagctg
    32761 tattccgggt tagccgcaat cgcgacatga taatggcatt tctgcaactc ttcattggtc
    32821 aagccgacgc gctcgcgacc aaacaccagc gccaccgggg tatttgccgc ttcagcgacg
    32881 cttttcaggc cgcattcgcg cgggtcgagc atcggccatg gcagcgtgcg ggagcgtgcg
    32941 ctggtgccca ccaccaggct acaaccggct aacgcttcgt cgagcgtatc gacgatgtga
    33001 gcattaccaa tcacatcgct ggcccctgct gccagggcaa tcgcctggga gtcgggtttc
    33061 accagtggat taaccagcca cagattggtt aatcccattg ttttcatggc acgggcaaca
    33121 gaacccatat tgccggtgtg tgacgtctcc accagcacaa ttcgaatatt ttgcagcatt
    33181 gtctttcttc atctaaagat tattcacgca tcttatcata aaacgaagac agatgccgat
    33241 ctcgctgcta tactctgcgc cgttttcccg ttctttaaca tccagtgaga gagaccgatg
    33301 catccgatgc tgaacatcgc cgtgcgcgca gcgcgcaagg cgggtaattt aattgccaaa
    33361 aactatgaaa ccccggacgc tgtagaagcg agccagaaag gcagtaacga tttcgtgacc
    33421 aacgtagata aagctgccga agcggtgatt atcgacacga ttcgtaaatc ttacccacag
    33481 cacaccatca tcaccgaaga aagcggtgaa cttgaaggta ctgatcagga tgttcaatgg
    33541 gttatcgatc cactggatgg tactaccaac tttatcaaac gtctgccgca cttcgcggta
    33601 tctatcgccg ttcgtatcaa aggccgcacc gaagttgctg tggtatacga tcctatgcgt
    33661 aacgaactgt tcaccgccac tcgcggtcag ggcgcacagc tgaacggcta ccgtctgcgc
    33721 ggtagcaccg ctcgcgatct cgacggtacc attctggcga ccggcttccc gttcaaagca
    33781 aaacagtacg ccactaccta catcaacatc gtcggcaaac tgttcaacga atgtgcagac
    33841 ttccgtcgta ccggttctgc ggcgctggat ctggcttacg tcgctgcggg tcgtgttgac
    33901 ggtttctttg aaatcggtct gcgtccgtgg gatttcgcgg caggcgagct gctggttcgt
    33961 gaagcgggcg gcatcgtcag cgacttcacc ggtggtcata actatatgct gaccggtaac
    34021 atcgttgctg gtaacccacg tgttgtgaaa gccatgctgg cgaacatgcg tgacgagtta
    34081 agcgacgctc tgaagcgtta atgactcagg cgggtgatgt cactcacccg ccctcgcctt
    34141 tcaggcgcta ttctgaaata cttcctcacc gctttacttt ctttcgtcac tctcccacca
    34201 tctttccccg attaatggat gataaagaag tcgcaaggaa aggttatggc actgccagtg
    34261 aacaaacgcg ttcccaaaat tctgtttatt ctctttgttg ttgccttctg cgtttattta
    34321 gtgccgcgcg ttgccatcaa cttcttctat tatcctgacg ataaaattta cggtcccgac
    34381 ccctggtcgg cggaatccgt cgaatttacg gctaaggacg gtactcgtct gcaaggctgg
    34441 tttattcctt cttcgacggg ccctgctgac aacgccatcg caaccatcat tcatgctcac
    34501 ggcaatgccg gaaatatgtc cgcccactgg ccgctggtca gttggttacc cgagcgtaat
    34561 ttcaacgttt ttatgtttga ttatcgcggg tttggtaaat caaaaggcac gccgtcccag
    34621 gccggattgc tggacgatac gcaaagcgcc atcaatgtgg tgcgccatcg cagtgatgta
    34681 aacccacaac ggctggtgct gttcgggcag agtattggcg gggcgaatat tcttgctgtt
    34741 attggtcagg gtgatcgtga aggcatacgt gcggtgatcc tcgactccac atttgcctct
    34801 tatgcaacca tcgccaacca gatgatcccc ggcagtggct acttacttga tgagagttac
    34861 agcggcgaaa attatatcgc cagcgtcagc ccgatcccgc ttttactcat tcacggtaaa
    34921 gctgatcacg ttatcccatg gcagcacagc gaaaagttgt atagcctggc aaaagagcca
    34981 aaacggttga tcctaatccc ggatggcgaa cacattgatg ctttttccga tcgccacggc
    35041 gatgtttatc gcgaacagat ggtggacttt atccttagcg cgttgaatcc gcagaactaa
    35101 cccatgatcg ctagcacgat aatcattcac aaaaccacct taagacatgc taatccactg
    35161 gtcagaacag tttaagatga gaaaaattct gtgacgcttg ccaacatttc tgatgattag
    35221 cattcccttc gccatttccc tgagcaaact ttagctattc ttatcaatta tgcttatggg
    35281 agatctacag atgatgccta cgcttgctcc accatctgtc ctttcggctc cccagcgccg
    35341 ctgccagatc ttgctgacgc tctttcagcc ggggttgacc gccaccatgg caaccttcag
    35401 cgagcttaat ggtgtggatg atgatattgc cagtcttgat atcagcgaaa caggacggga
    35461 gatcctgcgc tatcatcaac tcacactgac gactggttat gacggtagct accgggttga
    35521 aggtacagtg cttaaccaac gtttgtgttt atttcactgg ctacgacgtg gtttccgtct
    35581 gtgtccgtca tttattacca gccaattcac ccccgccctg aagagtgaac tgaagcggcg
    35641 cggaattgcg cgtaactttt acgacgatac caatctacaa gcgttagtga atctctgctc
    35701 ccgacggctg caaaaacgct ttgaatcgcg cgatattcat ttcctgtgtc tgtatctgca
    35761 atattgtttg ctgcagcacc acgctggaat tacgcctcag tttaatccgc tccaacgtcg
    35821 ctgggcggaa tcctgccttg aatttcaggt agcgcaggaa attggacgcc actggcagcg
    35881 tcgggcgctc cagcctgtac cgcctgatga gccactgttt atggcactac ttttttccat
    35941 gttgcgggtt cccgatccac tgcgggatgc gcatcagcga gacagacaat tgcgtcagtc
    36001 tatcaaacgt ctggtaaacc attttcgtga gctgggaaat gttcgttttt atgatgaaca
    36061 ggggttatgt gatcagcttt atacccacct cgcccaggcg ttaaatcgca gtttgtttgc
    36121 catcggtatt gataataccc tgccggaaga gttcgccaga ctgtacccac gcctggtgcg
    36181 caccacccgc gcggcgctgg ccggatttga aagtgaatac ggcgtccatc tttctgatga
    36241 ggaaagtggt ctggtcgcgg tgattttcgg tgcctggcta atgcaggaaa acgatctgca
    36301 tgaaaaacag attattctac tgaccgggaa tgatagcgag cgagaagcgc agattgagca
    36361 gcagttacgc gaactaacgt tactgccgct caacattaag catatgtcgg taaaggcatt
    36421 tttgcagaca ggcgctccgc gtggcgcggc actgattatt gcgccttata ccatgccatt
    36481 accgctcttt tcaccaccgc tgatctatac ggacctgacg ctgacaacac atcaacagga
    36541 gcagatccgc aaaatgctcg aatcagcatg agggaacaac tttcgggcgc aaaaacattg
    36601 ccggcagcgc caccagcgcc attacccaga acacgccgtg gcccagatat tgatacagga
    36661 aaccggcgaa aacggtcatg atagcgatac tgccgcccat cgcgacggca gagtaaaccg
    36721 cctgtaaacg gatgacttcg ctaccctggc gagcagcaat ataacgcatg gcggccaggt
    36781 ggcagaccgt gaaggtgccg caatgcagaa tttgcaccac tatcaaccac ggcaacgccg
    36841 tagttgctcc cataatgccc cagcgcacta cgccgcaaat cgccgagatc aacagcatat
    36901 cgcgtgcact acaacggcgg aaaagtttat tactcagcgc aaagataatg acttccgcca
    36961 ccacgcccag cgaccacaaa taccccaccg ccgaggccga gtagccagct gcctgccagt
    37021 aaatggcgct aaaaccgtaa taggccgcat gtgccccctg caataaacaa acgcaggcca
    37081 gaaagcgcca gttctggcga accagcgcca accacgcaga ccaaccggtg ctctcctgct
    37141 ggcgacttgc cccttgtggc tgaatcgtcg gacggatgag aaagccgagc agcatggatg
    37201 ccacgcccaa cgtcaacagc gcgaggatca cccgataatc aaacatagtg accagtttgc
    37261 ccgtcagcgc cgagccaatg acaaacgcca ccgagcccca cagtcgcact ttgccgtaat
    37321 caagcgggaa ctgcttttgc cacgtattcg ccagtgcatc ggtcaacggt accagtggtg
    37381 agaaaaagag gttaaagcca atcatcacca gcatcagcca cgctacgtgc gcccccgccc
    37441 agaaggcgac agcaaagaga agtgtcagca gtgccagcac gcgcaaggcg gaaatcaggc
    37501 gggaaggatc gctgacgcgg ggcgcgatga gcaaactccc gaggaaacgg gcaaccagac
    37561 ctgcccccaa taacaggccg atggtttctg gcgttaaacc aatcccttta agccagacgc
    37621 tccagaaagg tagaaaaatg ccgtaactaa aaaagtatgt gaaatagccg agcgccaacc
    37681 agcgcgtgga ttgcaaaacc atgagtccct cccgtcagga ggcgttagtc tggggttaat
    37741 cgaatgttgg tgcaagttga aaaataagaa tattcgagcc tgcttcccga tgtctgtaac
    37801 gccagttcac aagcactaaa aaactgagat ccgcgacgca tgttgcaaaa ataatgcgta
    37861 attatgccgt tacgcttgcc aaacgttcct gcacaatggc gatgaaatcg cgtaatgccg
    37921 gcttcatctc ccctttcttc cacgccatca gtaaagcaat ggaaggcaca tttccggcaa
    37981 tgggacgaaa aacaacctgt ccggtattaa aattattcat ataaccgggt atcaaagtga
    38041 cgcccagccc catgcccacc agattcatgg tcaccagaat attcgttgcc acctggacga
    38101 tatttggctg gctgttttct tgcgcaaacc acgctttaac gatcggcgca agcgaaccgg
    38161 aatacaccgg atcggtactg acgaaattca cgccatccag ttgcgcggcg gtgatctctt
    38221 tttcatgtgc taaagggtga tcaaccggta acacaaccac taatggttcg tcaaaaagct
    38281 ccagataatc aatctccggg ctataaacag gatggcgcat taagccgacg tcgagttcac
    38341 cacggcgaat tttttcctcc tgttgcgtgg tgattaaact caccagctca atcaaggtgt
    38401 ctggctgtct gagacgaaac atcggtaata cttttggcag taaattcact tccgccgatg
    38461 gcacaaagcc aatggttaat tgtctgtctt cctgaacaat tttccgcgcc cgtaatttgg
    38521 cattttccgc ttgttcgagg attgccagcg cgtcctggag aaaacattct cccgccgccg
    38581 tcaacgcgac tttgcgctta tccctcacca gtaacggaac accgacacag ttttcaagat
    38641 cgcggatctg gctgcttaac gaaggctgtg aggtatgcag tttttccgcc gcacgggtaa
    38701 agttcagtgc ctgcgccact gcgacgaaat agcgtaaatg ccgtagttcc atcaccttcc
    38761 ccttgttatc gaaaaaacgt ctcagctggt agaaattaac tatttcacat attagcaacc
    38821 aaccagcaac atccttatgg cacaaaaata gaaggtcaat acatcttatc tttcaggatt
    38881 aaaaaatatg accacaccct cagatttgaa catttaccaa ctgattgata cccaaaatgg
    38941 tcgggtcact ccgcgtattt ataccgaccc agatatttac caactggagc ttgaacgtat
    39001 tttcggccgt tgctggttat ttctcgccca cgaaagccag atcccaaaac ctggtgattt
    39061 ctttaacacc tacatggggg aagatgcggt tgtcgtagtg cgtcagaaag acggcagcat
    39121 taaggcgttt ctcaaccaat gccgccaccg ggccatgcgt gtgagttatg cagattgcgg
    39181 caacactcgc gcctttacct gtccgtatca cggctggtct tatggcatta acggcgagtt
    39241 gatcgatgta ccgctggaac ctcgcgccta cccacaaggg ttgtgtaaat cccactgggg
    39301 actaaacgaa gttccttgtg tggagagtta taaagggctg atttttggca actgggatac
    39361 cagcgcaccg ggcctgcgtg attacctggg tgacattgcc tggtatctgg atggcatgct
    39421 ggatcgtcgc gaaggcggca ccgaaattgt cggcggcgta caaaagtggg tgatcaactg
    39481 taactggaaa ttcccggcag agcagttcgc cagtgaccag tatcatgctc tgttcagcca
    39541 tgcttctgcc gttcaggtat taggggcgaa agatgatggc agcgataagc gcctcggtga
    39601 tggacaaacc gcccgcccgg tgtgggaaac cgccaaagat gcgctgcaat ttggtcagga
    39661 cggtcacggt agcggtttct tctttactga aaaaccggat gctaatgtct gggtcgatgg
    39721 cgcagtttca agctattacc gcgaaaccta tgccgaagca gaacaacgtt taggtgaagt
    39781 tcgcgccctg cgcctggcgg gtcataacaa tatattcccc acgctttcat ggctaaacgg
    39841 cactgccaca ctccgcgtct ggcatccgcg cggccctgat caagttgaag tgtgggcgtt
    39901 ctgtattact gacaaagccg cctccgatga agttaaagcc gcttttgaaa acagcgccac
    39961 tcgtgctttt ggtcctgctg gttttctcga gcaggatgac tcggagaact ggtgtgaaat
    40021 ccagaaattg cttaaaggcc accgcgcccg caacagcaaa ctgtgtctgg aaatggggct
    40081 tggtcaggaa aagcgccgcg acgacggcat tcctggcatt actaactata tcttttcaga
    40141 aactgccgct cgtggaatgt accaacgctg ggctgatctt ctgagtagcg aaagctggca
    40201 ggaagtgctc gataaaaccg ccgcttacca gcaggaggtg atgaaatgag tgcgcaagtt
    40261 tcactagagt tacatcaccg cattagccag tttctctttc acgaagccag cttactggac
    40321 gactggaaat ttcgtgactg gctggcgcag ctcgacgaag agattcgtta caccatgcgc
    40381 accacagtta acgcgcaaac acgcgaccgc cgcaaaggcg tccagccacc gacaacctgg
    40441 atttttaatg acaccaaaga ccagctggag cggcgaatcg cccgtctgga aacgggcatg
    40501 gcctgggcag aagagccgcc gtcacgcacc cgtcacttaa tcagcaactg ccagataagc
    40561 gaaaccgaca tcccaaacgt atttgctgtg cgggtaaatt atctgcttta tcgggcacaa
    40621 aaagagcgcg atgaaacatt ctatgttgga acgcgtttcg acaaagttcg ccgtctggaa
    40681 gatgacaact ggcgcttgct ggaacgggat atcgtcctgg atcaagcggt aatcacttcc
    40741 cataacctga gtgtactgtt ctgatgaatc gaatttatgc gtgtcccgtt gcggatgtgc
    40801 cggagggtga ggctctccgg atcgatacct cgcccgtcat cgccctgttc aacgttggcg
    40861 gcgagtttta tgccattaac gatcgttgca gccatggtaa tgcgtcaatg tcagaagggt
    40921 atctggaaga tgacgccacg gtggagtgcc cgctacacgc cgccagtttt tgcctgaaaa
    40981 cggggaaagc gttatgcctg cccgccaccg atccgctcac cacttatcca gtacacgttg
    41041 aaggtggtga cattttcatc gacttaccgg aggcgcagcc atgagcgatc tgcataacga
    41101 gtccattttt attaccggcg gcggatcggg attagggctg gcgctggtcg agcgatttat
    41161 cgaagaaggc gcgcaggttg ccacgctgga actgtcggcg gcaaaagtcg ccagtctgcg
    41221 tcagcgattt ggcgaacata ttctggcggt ggaaggtaac gtgacctgtt atgccgatta
    41281 tcaacgcgcg gtcgatcaga tcctgactcg ttccggcaag ctggattgtt ttatcggcaa
    41341 tgcaggcatc tgggatcaca atgcctcact ggttaatact cccgcagaga cgctcgaaac
    41401 cggcttccac gagctgttta acgtcaatgt tctcggttac ctgctgggcg caaaagcctg
    41461 cgctccggcg ttaatcgcca gtgaaggcag catgattttc acactgtcaa atgccgcctg
    41521 gtatcctggc ggcggtggcc cgctgtacac cgccagtaaa catgccgcaa ccggacttat
    41581 tcgccaactg gcttatgaac tggcaccgaa agtgcgggtg aatggcgtcg gcccgtgtgg
    41641 tatggccagc gacctgcgcg gcccacaggc gctcgggcaa agtgaaacct cgataatgca
    41701 gtctctgacg ccggagaaaa ttgccgccat tttaccgctg caatttttcc cgcaaccggc
    41761 ggattttacg gggccgtatg tgatgttggc atcgcggcgc aataatcgcg cattaagcgg
    41821 tgtgatgatc aacgctgatg cgggtttagc gattcgcggc attcgccacg tagcggctgg
    41881 gctggatctt taaggaagca cgatgaaaga aaaaaccatc attattgtcg gtggcgggca
    41941 agcggcggca atggctgcgg cctcgctacg ccagcaaggg ttcaccggtg agctgcatct
    42001 gttttccgat gagcgacatc ttccttatga acgacctcca ctctcgaaat ccatgttgct
    42061 ggaagattcc ccgcagttac agcaggtgtt acccgctaac tggtggcagg aaaacaatgt
    42121 tcatctgcat tccggtgtaa ccatcaaaac gctgggtcgc gacacacgag agttagtgtt
    42181 aaccaacggc gaaagctggc actgggatca gctttttata gcaaccggcg cggcagctcg
    42241 accgctgccg ttgcttgatg cactgggaga acgctgcttt accctacgcc atgccggtga
    42301 tgccgccaga ctgcgagaag ttctgcagcc cgaacggtca gtcgtgattg tcggtgccgg
    42361 aactattggt ctggaactgg ctgccagcgc cacgcagcgc agatgtaagg tgacagtgat
    42421 tgaactggcg gcaaccgtca tgggccgtaa tgcaccaccg cccgtgcaac gctatctttt
    42481 acagcgccac cagcaggctg gtgtgcgcat tctgctcaat aatgccattg aacatgtggt
    42541 cgatggtgaa aaagtagaac tgacgctgca aagtggggaa acgcttcagg ctgatgtggt
    42601 gatttacggt attggtatca gcgccaacga gcaactggct cgcgaggcca accttgatac
    42661 tgccaatggc attgtcattg atgaggcttg ccgcacctgc gatcccgcga tctttgccgg
    42721 tggcgatgtg gcaatcactc gtcttgataa tggtgcacta caccgctgcg aaagctggga
    42781 aaacgccaat aaccaggcgc aaattgccgc tgccgcaatg ttagggctac cgctaccgct
    42841 actgccgccg ccgtggttct ggagcgatca gtacagtgat aacttacagt ttattggcga
    42901 tatgcgtggc gatgactggc tttgtcgtgg caacccggaa actcagaagg cgatttggtt
    42961 taatctgcaa aacggcgtgc ttatcggtgc ggtcacgctg aatcaggggc gtgagattcg
    43021 cccaattcgc aaatggatcc agagcggcaa aacgtttgat gcgaaactgc tgatagatga
    43081 gaacatcgcg cttaaatcac tgtaaccagg ataattagcg aatatctcaa tgcctggggc
    43141 gtggcgaggt gcaagagtgt gtattacgtt taaatcacat tatcttgcaa agggattggt
    43201 tatgaacaca ctacgttatt ttgattttgg agctgcccgc cccgttttgt tattaattgc
    43261 ccgtatcgcc gtggtcttaa ttttcattat ttttggtttt cccaaaatga tgggctttga
    43321 cggtacggtc caatatatgg cctcgttggg cgcgccaatg ccgatgctgg cagcgattat
    43381 tgcggtagtt atggaagtgc ccgccgcgat attaatcgtg cttggctttt tcacccgtcc
    43441 gctggcggtg ctgtttattt tctacacgct gggtacggcg gtgattggtc accattactg
    43501 ggatatgacc ggcgatgcgg ttgggccaaa tatgattaat ttctggaaga atgtcagtat
    43561 cgctggcgca ttcctgctgc tggcaattac cgggccgggg gcaatttctc tcgatcggcg
    43621 ttaggaaaga tgccggatgc ggcgtgaacg ccttatccgg catttaataa attacagcca
    43681 ctctacccgc aacgacatct ctgaagttgt tgattcccct ggtgctagcg caatgagatc
    43741 acccccttcc gggcgatgat gatcatccgg cgcatggctc atcggttcca gacagaagaa
    43801 atcaaacgca tatcctttat caaacgcagg gtcggaaaca aagatgaaat aacacggtgc
    43861 aggtggcgtc gtttccatga tgatggcata accttcctgc ggctgctcga tgcgggcctg
    43921 accattccat ccggcaaaac cattgttcac ccactggcgc ggcaacggcg cgggctggtt
    43981 aaaatccagt tcctgcggta gctgctcgca aaactcaccc gccagccact gctcccgctc
    44041 cagccagtaa ccgctcgcct gtgcctgaat ccgcgtttgc ggcgacaacg gaaaataagg
    44101 atgccagccg gtaccaaatg gcagcgtctc tgccccttga ttggtgacag agagcgtcac
    44161 cgtcagcgta tccgccgtta aatgaaacgc ctgacttacc cgatagtgat agacaccgct
    44221 gcgatgttca tacaccagac acaaactatc atcgctgtgc gagacacatt gccactcgcc
    44281 cagccagcca tcgccgtgta gatagtgcgc atcccactca acattcggtt gcagttgata
    44341 ctcacgcccc tgccagacaa aacgattgcc gctcacccgg tttgcaaatg gcaccagcgg
    44401 aaaacatgag gcatcggttg ccacaccgct ttttttacca ggacgtaaga gcggcgtcgt
    44461 atcgcgccag aagccttcga ttacgccgcc ctggtcagaa acgtccagct ttagcgaccc
    44521 gtgggataag gtatagatgg tcatccgcta actccttaat ccgggaagtt aatcacaact
    44581 ttcccgcatt tgccgctcgc catcagcgca taggcatcac ctgcctgttc cagcgagaag
    44641 cgatgggtaa tggcgttacg cggccacagt ttccagtccg tcagatcatg ggcgcatttt
    44701 tccatatgga acagactggt cacccaggag ccgataatcc gccgttgatg gtgcatcaga
    44761 tcggcgctga cctcgaactc cacttttccg gtttctccga tgtaaaccac ccgtccccag
    44821 tcagcggtgg atcgcagtgc cagcaagcga cctgcggcat taccagaaca atcgagcgca
    44881 acatccgcgc caccgtgggt gagttcggcg ataatctgcg gcagaccttc ggtggttgct
    44941 aaatagccgt gatccatcac ccctaactgt tttgccatcg ccagacgttc cggcagcata
    45001 tcaacgccga tgatccgttt tgcaccgcga cctttcgcca gcatcatcgc catcatgccg
    45061 actggcccca gaccgaccac cagcacgtta tcactgccgg aaacttcgcc gcgcaaaatt
    45121 ccttcatacg ctgtaccaac gccgcaactg ataaacgcac catcttcgta gctcagcgca
    45181 tccggcagga ggatcagatc tttttcttcc gccagcagat attcggcatg accgccgtca
    45241 cgctgccagc cgtaagccgc ttttcctttg ccagtacaag agataggaaa gccgcggcgg
    45301 cagttcgggc aaaaaccaca gccagaaatg tgatacacca gcacgcggtc gccctcttta
    45361 aaatggcggc agccttgccc catcgccaca atctgcccgc acggttcatg accgttgata
    45421 aatccctggt ataacggttt atcgggtgcc gccgccgtgg cacggtgttg atgatagata
    45481 tagtggacat cgcttccgca aatcccggag gatttcattt tgatcagtac ctggttaatc
    45541 cccggcgtcg gcaccgcaac ttcccgcaga tcgacggttg aatttcctgg taaataagct
    45601 gccagcatcg ttttcataaa tccctcatta acaatacgat taattttcat ccctgccgca
    45661 cccgcgccag gggcgttaag gtttagcgtt tcgctttact gcttcgctgg gtcagcaaga
    45721 tattcgccag caccgccacc acgatgatga cgccgcgtac cacctgctgg aaaaaggagt
    45781 taataccgag cagcaccaga ccgttaccga ttagcgtaat caccagcaca ccaagcaatg
    45841 taccgaacag ggaaccgcga ccgccggaaa gtgccgtacc gccgaccacg accgcggcga
    45901 tgacgtcaaa ctccagaccg tttgcggcac ctgcgttacc ggaaccgagg cgtgccgcca
    45961 gcaaaatgcc ggtcaccgcc gctaataatc ccgaaagggt aaagataaga atgcgcactc
    46021 gacgaacgtt aataccgcac aactgcgccg ccgtggcatt accgccaacg gcaaaaaccg
    46081 agcgcccgaa ggcggttttt cggctaatga acacaaacag cgcaaacaac accatcatga
    46141 tcagcgcgga tactggcaca ccgagaaatt gtccgcccag ccagtccagc acctcgtttt
    46201 cgtcaatcgg cactggcagt gcgttggtca taaacagccc cattccgcgc agggcgctcc
    46261 acagccccag tgtggcaacg aaacttggta cgttaaacac gccgcgcagc accccggcga
    46321 gcgttcccat cagcgcgcct aacagcaaca ccagcagaca cgccaccgcc agcggaactt
    46381 caaattgcag caaaaatgcc aggcacaccg agacaaaagc caccatcggc ccaacgctga
    46441 catcaatttc accggagata ataatcagcg tcatcgccca ggcggcaatc ccaatggtgg
    46501 cggcatcgcg cagcacgttc atctggttat tcaatgagat aaagcctggc gcgttcaggg
    46561 agaagaccag ataaagaatg gcaatcacca ccagcaaacc gatctcatta atatggcgac
    46621 tgacaaattg tttgagcgag acgctcttgc cctgcggcaa tggtaatgac gaagcagaca
    46681 tcttcaggtt cctcggtaaa attcatcagt gcacagacag aatggcggac atcagctcat
    46741 ccacattgac cggagcgtga aactcctgcg agaacgtgcc gtgctgtagt aacaggatgc
    46801 ggtcacagac cagcggcaac tcctccactt cactggagat aaacaccacg ctttttcctt
    46861 cggcagccag ctcacggaca atacggtaaa tctgctgttt ggcttcgata tcgacgccgc
    46921 gcgttggctc gtcgagcaac aaaatctggc tggcggcata gacccaacga ccgatcacca
    46981 ctttttgctg attgccgcca gaaagcgtgc cgatgggcgt ttcgctactg gcggccttga
    47041 ccgtcatccg ctgcatcacc tcttcggtca ggcggcggat ggtggaccat tgcagtacgc
    47101 cgttggcgct gattttttgc cgattggtca gcactgtatt ttcgtcaacg cccaaccagg
    47161 gaatgatccc cgcttctttg cggttttctg gcgtatagcc aatgccgcgt ttcagcatgt
    47221 cgccgtaatc ggggcgcgtg attttctcgc cgttgataac aatttcgccc tgttcatact
    47281 cctccagccc aacaatcgcc ttcagcaatt cactgcgccc tgcccccagc aggccagcaa
    47341 tgccaagcac ttcgccgcga cgtaacgtaa agctgatatc ctccagcttg ggcttatggc
    47401 gtaatgcacg gacttccagc acggcctgat ccacaatttc ctgaggggca accggcgcaa
    47461 tatcaacgtg atcacgccct agcattagcg acacaatatg atgcgtggag gtgttttcga
    47521 gcatcacatc gcccgccacc tgaccatcgc gcataacggt ggcacaggag gcaatgcggc
    47581 gaatttcttc catccggtgg ctgacataaa tcaccgccac gcccagtgcc gacatctttt
    47641 tcaccgcgat gatcaccagt tcaacttccg cactcgcaag cgaactggta ggttcatcaa
    47701 gaatgaccac gcgcggctcg cccttcatca cccgcgcaat ttccaccagt tgcttttgcg
    47761 ccgggcttag cgttgaaaca agttgttcag ggctaacgtc aacgcccagc gcctgtaagc
    47821 aacgttgggc atcctgcgcc atttgcaggt aatcaatcat gccgttgcgg cggggccact
    47881 gaccgaggca gaggttttcc gccaccgtca gtccctccac cagacttaac tcctgataaa
    47941 ccgcacgaac ccccagttca gcggcacggc gagtcaacgt agcttcgtca ccttccagtc
    48001 gcgtctcgcc aatccagata tcaccgctat ccgggcgttc gctgccggta agcattcgaa
    48061 tgagagtcga tttgcccgcg ccgtttttac ctaacagcgc acgaacttcg cctttattga
    48121 gcgtgaagtt aacgttatcc agcgcaacga cgccggggta acgcttattt cctgccacca
    48181 cttttgctac cgggactgcc tctgttgccg tgaacatagg gacctctgcg aatcagcgat
    48241 tagggcagac catcaacgtg cgttgccagc cactgtttac catcttccgt tttggtatag
    48301 agatcgattg gcacctgaat cactttttca ccgtcggctt gtttattgat aaccttcaat
    48361 gtttgcgcga aaacagcatt gcccattttc ttaccggaaa tatccactac cgctttcagc
    48421 acctgattgt tttccagctc ctgagcaatt tcggttgtca tatccgaacc gaaaacagca
    48481 atttttccgg cctgattttg attacgtacc gcttttaccg cgccgagtgt cgcaccgccc
    48541 gactccccca taatggcgtt gagatccggc gtggagataa tcagtttttc accaacggaa
    48601 atcgctttat ctaaaacagt cccttcctga ttagcgacaa tttgcgcgcc gggaacgcgg
    48661 gattttaata cttcttcaaa tcctttacgt cgctgcacac aaacttcaaa ggcttcgcaa
    48721 ttgatgacag caattttcgg ctggtcaatt ttattggcaa taaaataatc ggcggcagcg
    48781 ttacccagtt ttttaccaaa ttccagcgga tcgccgacca gatacgccga gacatattta
    48841 tcgcccccct tttgattaat acaggtgttg tagcaaatca ccggaatacc cgcttcactg
    48901 gcgcgacgaa cggtacggct actgccattt tcagacactg ccgataaaat aatggcatcg
    48961 acattacgcg ccacgagggt atcaacaaag gtactttctt tcgaaatatc accctgggcg
    49021 ttagtttcaa ttaactgtac ctgtactgaa gaatcttttg ccgcatcctg aacgccctga
    49081 cgcactccag cgtaatatcc ctgggtatca aggtatattg caccaatggt catttcttta
    49141 tccgcagccc tggcaaatag tgcgctacct aatagcgtag ccattaataa tagattacgg
    49201 gttgttctca tttttttagg catagtgcct cctgtagggt ttttattaac aacggcttat
    49261 tctaattatt ttgtgatgag cggcagcgcg tgccgctcaa atattacagt taatagggtt
    49321 tagtgaacgt agttaaaaat aaaaggcatc acggtggtga ataatgccgc tttcggccag
    49381 gccggattaa tcgccgtcaa ttcagcgcga gcagattcat attgcgcaac ctccccgagt
    49441 cccgcggacg ccagcatcgc caccatcagg catttttctt tatgctgctg ttgtaaatcg
    49501 ccatacagcg acaacaggtc aggttgtgaa acagcaaaga aatccgcctc aatactggtt
    49561 ttcgccatct cttgcgccca ctgtttcatt tcactaaaca gttgctgtgc ggtttgctgt
    49621 tcacccagca gtcgcagcgc cattccttgc cagaagagat aatcaaccgg ctgatcgttg
    49681 taataactgt ggatgttaat ggtgcgatcg ccggtcgccg ccagacgtaa acaacgcatc
    49741 gcttcagttt catcgccctg cgcgttggcg catatcgcct gccagaacca gatgtcgtta
    49801 tcagtttgcc ccggtaaacg gccttcgctt aaattctccg gataatgcag cgcggcatga
    49861 agcagttcgc tggcctgctg cggctctctg gcatcaagat gctgccaggc gcgtaataac
    49921 tggttgagga taaactgact ggtgaccttc ccttccccgc cttcccacgg gtggaattta
    49981 cgcgtggcga gaatgtccgc cgctttgtct gcctgacccg tgagatgcca caaattgagc
    50041 agttctgcgg tcatgtcgtc gcgtttcagc gcaatttcct gattattttc cagacgcgcc
    50101 agtcgtttct ccggtgtggc tccacttaac ttatcaagca aatcccgttc gaaaagcaga
    50161 cgtgcatcct gcggcgcaag ctgataagca ttatcaagat aacgcgcggc cagctcataa
    50221 tcgtgttgct tattccacgc atggatcgct aacccgcgcc agccgtcggc aaactccggc
    50281 gacatctcta cgcaacgttg ccataaggca atggctttgt tgtagctacg tttgttgtag
    50341 tagaagcagg ccagtaaatg gcgagcaaac cagcactctt caatactctc cagcgccgcc
    50401 acttcttcca gcgtattcgg gaaacggaca aactgcggga agacatcaat ggcttttgcg
    50461 accagttcgc cacgttcggc tttcggcagc aagctggctt gcaggtaaag cggcagcgtg
    50521 cgctggcagt ccagtgcgtt cagcatctct gccgccaggg tgggcattcc ccagttaatc
    50581 agttgcccgg cggtcatcag ggcgttaacg tcgcgtccct gacacagacc gcgccactgc
    50641 gcgagagctg attcgctacg accatcgaac cagttcagcc accacagagt ggcgttcagc
    50701 ggataatcgc gcagcagttt ctcgcgctgc acacgcgcgt tgtcctgacg accacttaac
    50761 accagcagca gattatgcag gcaaagcact tcctgattgg ttgggcaggc gcgaagactt
    50821 tgttggcaaa aatccagacc agcgtcgaag ttaccattac gcgccgccag tcgtgccagg
    50881 ccataatagc caccggcttt gctgttgccg ctccagaccg cacgccagaa atcctcttcg
    50941 gcttgttgat attgtccctg acgttcgtaa gcactggcgc gaatcaaact cgcctgtccg
    51001 cactgcggat ttttgttcag cgcatgtgcg cgtttcagag cctgagtggc atacgccacc
    51061 gcttgcggga aatcggcgcg gttatattcc agcatcgcca gcgccaggtt acagcggtaa
    51121 tccagcggat ccagcgccac gccgcgcagg tagtaatcga acggtgaacg gctggcgtga
    51181 tgatattgct ccagatgctg accgataaac caggcttcat ctgtactggt aatgtcttgt
    51241 gctgccagtg gcgctttggc gacgtccggc aacggcaact cttgcggctg atgttcctga
    51301 taactcagta caatattgcc gtcggcatcg gagagctcaa tggtcagcct ttcaggattg
    51361 ataccgtgca acacgccctg gatggcggtc gcaggcatca gtgccacggc atcatcaagt
    51421 aacgcgttgc atttgccgat ttcgcggatc gccaggcggt atccgttcaa cggagagatg
    51481 gcatacagcc cccactcaat cccccgctta ctacgctgga gttttatcac cgcatcgcgg
    51541 gaggcatttt gcaccatgcc caaagaatga taaggcagga aatactgctc aaaacgcttc
    51601 tcttcgtaag catcaagcca ggtaaaatcg ggctggttat cggcaaaaat accggtcatc
    51661 agttcgatat acggaccgtt attgtcggtc agactcttat cccacgcctg gccaaattca
    51721 ctgtgtcccc aactccactg ttttttacct ggcgcaatat ggtggttggc aacgtgcagc
    51781 aaaccgccat cttcatcgtg acaccacgcg ccgacaaaat cgtactgcga tttttcggcc
    51841 atatatgagg ttggaacagg cacattttta tagcgagaaa tgtccactcc ggcggagtag
    51901 tccactttgt agtaagtgcc ggtggcgatg gggaaagcgg agacggcccg tttgccgtga
    51961 tcaaacaccg cagtcacatc cggtgggaaa acgctctgat gcccttcccc ccctttcact
    52021 gccgggttgg cccaccacaa gaaatgacgc ggcgtggcgt taccgttata gacgcggctg
    52081 gcgatttcca gcgccgcccg gtcagggcgc agggtgaaac ctgtcatcac ctgtaaacca
    52141 tgcatcggct ccgtttcgcc tacccacacc gtctgtgcac cgtcttcatg ggcttcgagg
    52201 gtgaaatcaa cgggcataaa ggtggtcggg cgatgatgtt gcggccagtt aaactcaatc
    52261 ccaccggaaa tccacggccc cagcagcccc accagcgcag gtttaatgac ttcattgtga
    52321 taaacaaaat cgcgctgttt cactttatcc cacgcgcgat gcacccgacc gcccagttcc
    52381 ggcaggatca tcactttgat gtagtcgttt tccagccaca ccgcctgcca ggatttcagg
    52441 gttttctgct cgctcagcgt atcggtcacg ccgtagggat aaaccgcgcc ggacgatccc
    52501 tgataaacgc gattttcgag gaacatggga tgtatatcct gcggcccggt ttcataggtc
    52561 gggatctcaa cgcgctcttg ccacactttt actggagtca tggtgccctc aatattaaca
    52621 agacatactg aattaaaaga tttgtggcag tgtattgaac aatctggcaa tgttttcgcg
    52681 gaataatcac gcaattaact aaacaaggtt tagtgaagat gagagcctgc attaacaatc
    52741 aacagattcg ccaccataac aaatgcgtga ttctggaact gctgtaccgg caaaagcgcg
    52801 ccaataaatc aacgctggcc cggctggcgc aaatttcgat tccggcagtc agtaatattt
    52861 tgcaggaact ggaaagcgaa aaacgggtgg tgaatattga cgatgaaagc cagacgcgcg
    52921 ggcatagtag cggtacatgg ctgattgcgc cggaaggtga ctggacgctg tgcctgaacg
    52981 tgacgcccac cagtattgag tgtcaggttg ctaatgcttg tttaagtccg aaaggtgaat
    53041 ttgagtattt acagattgat gcaccgacgc cgcaggcgct gctgtccgaa atcgaaaaat
    53101 gctggcatcg ccaccgtaaa ttgtggccgg accgcaccat caatctggcg ctggcaatcc
    53161 acggtcaggt tgatcctgtg accggcgtgt cgcaaaccat gccgcaagcg ccgtggacaa
    53221 cgccggtgga ggtgaaatat ctgctggaag agaagctcgg cattcgggtg atggtcgata
    53281 atgactgcgt gatgctggcg ctggcggaga aatggcaaaa taattcgcag gaacgggatt
    53341 tctgcgtgat caacgttgat tacggcattg gctcgtcgtt cgtgattaac gagcaaattt
    53401 atcgcggcag tttgtatggt agcggacaga ttggtcacac catcgttaat ccggatggcg
    53461 tcgtctgcga ctgtggacgt tatggctgcc tggaaaccgt cgcctcgtta agcgcattaa
    53521 aaaaacaggc gcgggtatgg ctaaaatcac aaccggttag tactcaactt gatcctgaaa
    53581 aactgactac agcgcagtta atcgctgcct ggcaaagtgg agaaccgtgg atcaccagct
    53641 gggttgaccg cagtgccaat gccattggtt tgagtctgta taacttcctc aacatcctca
    53701 atattaatca gatttggttg tacggtcgca gttgtgcctt tggtgagaac tggcttaata
    53761 ctattattcg ccagacagga tttaacccgt tcgaccgcga cgaaggaccg agcgtgaaag
    53821 cgacgcaaat tggcttcggg caattaagcc gcgcacaaca ggtgctggga attggctatt
    53881 tgtatgttga ggcgcagtta cggcagattt gatggcgcaa taacgtagaa aggcttcccg
    53941 aaggaagcct tttttcaaca gattaaccgt ttcgcttatg cgtaaaccgg ataacgtgcg
    54001 cagatgtcga gaactttacc tttgatgcgc tcgataacag cttcatcatt gatgctgtcc
    54061 agcacgtcac acatccagcc agccagttct ttcgcttctg cttctttgaa gccgcgacgc
    54121 gtaattgccg gagtacccac gcgaataccg gaggtcacaa acgggctctt cggatcgttc
    54181 ggtacgctgt ttttgttgac ggtgatgtta gcacggccca gagcggcgtc tgcttcttta
    54241 ccggtcaggt ttttatcaac cagatcaacc aggaacaggt ggttatcagt gccgccggaa
    54301 accactttgt agccgcgctc gaggaacact tctaccatcg ctttagcgtt tttcgcgacc
    54361 tgctgctggt aagttttgaa ctcaggctcc atcgcttctt tcagagcaac cgctttaccg
    54421 gcgattacgt gcatcaacgg accgccctga ccaccaggga aaacggcaga gttcagtttt
    54481 ttgtacagct cttcgctacc acctttcgcc aggatcaggc cgccgcgcgg acccgccagg
    54541 gttttgtgag tggtggtagt aacaacgtga gcatgaggaa ccgggttcgg gtagacgcca
    54601 gcagcaacca ggcccgcaac gtgcgccata tcaacgaaca ggtaagcacc gatgctgtca
    54661 gcgatttcac gcattttcgc ccagtccacc acgccggaat atgcagagaa accaccgata
    54721 atcattttcg gcttgtgttc tttggcttgt ttttccagat cggcgtagtc gatatgaccg
    54781 gtagcatcga taccgtaagg aacgatgttg tacagtttac cggagaagtt aaccggagaa
    54841 ccgtgagtca ggtgaccgcc atgcgccagg ttcataccca gaacggtatc acctggttcc
    54901 agcagcgcgg tgtagaccgc aaagttagcc tgggagccgg agtgcggctg gacgttagcg
    54961 tagtcagcgc cgaacagttc tttcgcacga tcgatcgcca gttgttcaac gatatcaaca
    55021 tactcgcaac cgccgtagta gcgtttgccc ggataacctt cagcatattt gttggtcagc
    55081 tgagaaccct gcgcctgcat tacgcgcggg ctggtgtagt tttcggaggc gatcagttcg
    55141 atgtgctctt cctgacgtac tttttcctgc tccatagcct gccacagttc ggcatcataa
    55201 tcggcaatgt tcatttcacg ctttaacatc cgcatctcct gactcagcta acaataaaat
    55261 ttttggcctt tataggcggt cctgttggac aacggcgaac agtataaccg aatcattgtg
    55321 cgataacagg tcttgacaaa ggaatttacg caaacgatta ccttcaggct acgcaaggct
    55381 ttggagaata aagagcttgc aaccggaaac ggatttcttt tcaggtttgt gatgcaaatt
    55441 tttcacttca tcacattctt tctgaaaaac accaaagaac catttacatt gcagggctat
    55501 tttttataag atgcatttga gatacatcaa ttaagatgca aaaaaaggaa gaccatatgc
    55561 ttgacgctca aaccatcgct acagtaaaag ccaccatccc tttactggtg gaaacggggc
    55621 caaagttaac cgcccatttc tacgaccgta tgtttactca taacccagaa ctcaaagaaa
    55681 tttttaacat gagtaaccag cgtaatggcg atcaacgtga agccctgttt aacgctattg
    55741 ccgcctacgc cagtaatatt gaaaacctgc ctgcgctgct gccagcggta gaaaaaatcg
    55801 cgcagaagca caccagcttc cagatcaaac cggaacagta caacatcgtc ggtgaacacc
    55861 tgttggcaac gctggacgaa atgttcagcc cggggcagga agtgctggac gcgtggggta
    55921 aagcctatgg tgtactggct aatgtattta tcaatcgcga ggcggaaatc tataacgaaa
    55981 acgccagcaa agccggtggt tgggaaggta ctcgcgattt ccgcattgtg gctaaaacac
    56041 cgcgcagcgc gcttatcacc agcttcgaac tggagccggt cgacggtggc gcagtggcag
    56101 aataccgtcc ggggcaatat ctcggcgtct ggctgaagcc ggaaggtttc ccgcatcagg
    56161 aaattcgtca gtactctttg actcgcaaac cggatggcaa aggctatcgt attgcggtga
    56221 aacgcgaaga gggtgggcag gtatccaact ggttgcacaa tcacgccaat gttggcgatg
    56281 tcgtgaaact ggtcgctccg gcaggtgatt tctttatggc tgtcgcagat gacacaccag
    56341 tgacgttaat ctctgccggt gttggtcaaa cgccaatgct ggcaatgctc gacacgctgg
    56401 caaaagcagg ccacacagca caagtgaact ggttccatgc ggcagaaaat ggcgatgttc
    56461 acgcctttgc tgatgaagtt aaggaactgg ggcagtcact gccgcgcttt accgcgcaca
    56521 cctggtatcg tcagccgagc gaagccgatc gcgctaaagg tcagtttgat agcgaaggtc
    56581 tgatggattt gagcaaactg gaaggtgcgt tcagcgatcc gacaatgcag ttctatctct
    56641 gcggcccggt tggcttcatg cagtttaccg cgaaacagtt agtggatctg ggcgtgaagc
    56701 aggaaaacat tcattacgaa tgctttggcc cgcataaggt gctgtaattt gatgttgccg
    56761 gatggaaaca tccggcaacc cttgacgcgg tttaaattgc cgcgtcgtcc tcttcaccgg
    56821 tacggatgcg aatgacccgt gccacgtcga agacgaagat tttaccgtca ccgattttgc
    56881 cggtttgcgc cgtgcgaata atggtatcaa cacaggtatc gacaatgtcg tccggtacga
    56941 caatctcaat tttcactttc ggcagaaaat ccaccatata ctccgcgccg cggtacagct
    57001 cggtatggcc tttctggcgg ccaaaacctt tcacctcagt gaccgtcatg ccggtaatgc
    57061 cgacttcagc cagtgcttcg cgaacatcgt ccagcttgaa gggtttaata atcgcatcaa
    57121 tctttttcat gctattcctt gaaaaggtcg cctgtctttt gatctgctaa acgtaacaca
    57181 taacgccaat tcattccttg aaatcgtttg catccagctc gtgtcgggaa agcagtttat
    57241 aaaattctgt ccggttgcgc cccgccattc tcgccgcgtg tgtgacgttg cctttggtga
    57301 tttgcagcag cttacgcaaa tagttgagtt caaactgatt acgtgcctca acaaaggttg
    57361 gcagcgccgt attttcaccc tccagcgcct gctctactaa tgcatcgcta ataaccggag
    57421 atgaggtcag cgccacgcac tgttcaatca cgttgaccaa ctggcgcaca ttacccggcc
    57481 agctagcggt catcaggcgt ttcatcgcat cagtggagaa cgcgcggaca aacggtttat
    57541 gtcgctctgc cgcctggcgc aacaggtgat ttgccagaag cggaatgtct tctgtgcgct
    57601 ctgccagagc cggaattttc aggctgacaa cgttgaggcg gtaatagagg tcttcacgga
    57661 attccccgcg cgccatcgct tttggcaggt cgcggtgagt ggcggaaata atccgcacgt
    57721 cgatatcaat atcgcggtta ctgcccagcg ggcgcacttt acgctcctgc aacacgcgca
    57781 gcagtttgac ctgcaacggt gcgggcatat cgccaatctc atcgagaaat agcgtaccgc
    57841 cttccgccgc ctggaataaa ccttcgcgat tgctgacagc gccagtaaac gcgccacgcg
    57901 catgaccaaa cagctccgac tccagcaatt gctcgggcaa tgcgccacag ttaatagcaa
    57961 taaatggttt gctattgcgc gggctggcgt tgtggatagc ctgggcgaaa atctctttcc
    58021 cggtgccgct ctggccgtta atcaaaacgc taacgtctga ttgcgccacc agccgcgcct
    58081 gttccagcaa acgcagcatc agcgggctgc gggtgacaat cgcctcgcgc cagcgttcat
    58141 cggtggctgg cgcggattgt tccagcgcat cgtcaattgc ctgatatagc gcatctttat
    58201 cgacaggctt ggtgaggaaa ctgaacacgc cctgctgtgt tgcagcaacg gcatcgggaa
    58261 tagaaccatg cgcggtaaga ataattactg gcattcccgg ctgcactttc tgaatttcag
    58321 caaacagctg cataccgtcc atttcatcca tccgcaggtc gctgatgact aaatctactt
    58381 tttcgcgatt cagtacccgt aatccttcag cgccactttc cgccgtgacc acgctgtagc
    58441 cttcgctggt caggcgcagg ccaagcagtt tcagcaatcc cggatcgtca tcgaccaata
    58501 ataaatgcgc aggtttatgg ctcatcagga gtgacctcat gggtggatgg cgcgggcttt
    58561 tcaccctcgt gcggcgtatc cggcgagaaa tttccagccg gtttgcgggt cgagagctgg
    58621 cgttcaatat cagtcaggtt ttccagcttg cgggtggtga gttccagttg ctgctgtaga
    58681 acgtgatgtt gctggcgcaa tgtatccagc tcgctgtcgc tggcctgctg gagcttgccg
    58741 tagcgttggc gctcttccgc cagttgcagt tgcagcgcct gaccgtcgcg ccagagttga
    58801 tacagagggc gaacctgtgc cgggatctcg gtacttagcg catcaatgcg cgcgaccagc
    58861 tggcggcgct catacggcgt aattttggcg tcggcgagca aaatcccttg tttaaaggta
    58921 ttttgccagc tgccgtcgtc atattggcga gcttgctgac gtgactgcgc aggcattaaa
    58981 cgatcagcac aatccatcgc ccgcagccag taaagcggat tggtttcggt tgatttacct
    59041 tgcagcgccc agatgtcgct acattcagta gaaagatagt cagccagttg ataaaccgga
    59101 attttttctt ctgctggcgt atcaatggct ggcttattgt gactctgcac gcaacccagc
    59161 aatgccagac atggcagccc tgccagccac agccgtcggg gcaataatcg ttgaaaaatg
    59221 tgtcgcatat tcaccagact taatgcctat cccagtgggc gtaattattg caggcagttt
    59281 ggacatggac agcgcggaga aaccggagcg tacacgtcgt acgtgaggat ttcgagcact
    59341 gcccggggcc gaactggcaa ataaaatagc ctgatgggat aggctgttag cattatttcg
    59401 tgtttttcga cgacggcaat tcaatgcgga aacaaacgtc ttgcccgctc tcgtcgacca
    59461 gatacagttc cccttgcata cggcaaatac aatccctggc aatgcttaat cccagaccgc
    59521 tgcccttcac cgcccctttt cgctggtggc ttccttgaaa aaagggttcg aagatcatgg
    59581 cgcgttcctc ttgcggaatg ggtgtgcctg tattgatgac atcaatataa acccgtgcac
    59641 catgtaagct gctgcgaagg caaatgttac cggattcagc cccgtagtgc accgcattgg
    59701 agtaaagatt atccagtacg ctcatcagca gcattggctc cgccaggcaa gctgttgctt
    59761 tgagatcgac gtcggtatgc atcattttag cccgtgcggg caggctatga gcagaaacca
    59821 ctgtctccac cagcggtgct aattcaacat tctccagttc caccgcactg tccgcctgtt
    59881 tacggttgta atcaagcagt tgttcgatca gtttttgcaa attgcggctg ctgctatcaa
    59941 gaatgctcac cacctctttt tgctctggcg taagcggccc gacaacctgg tcagccagta
    60001 attcagtgcc ctcgcgcata ctcgccagtg gcgtttttaa ttcatgagat aaatgtctta
    60061 aaaattgatg gcgttgggat tccagccatg acaggcgctc acttaaccaa agaatacgtt
    60121 gcccaaccga gcgtaactcg ctcggtccac tgaacgaaac gctattgccc agagaacgcc
    60181 cttcccccag acggttgatc atgcgctcga tatttttcac cggcccgata atcatccgcg
    60241 tgaaaagtag caccatcacc agactcacca gaaatagcac cagcgattgc caaccaaaat
    60301 attgcccacg ttcggcgatt tcacgctgaa gttgctgccc acgagagaac accactgtgc
    60361 gcgtggcctg caccatttcg gtattggcac tggcaaaggc ttcaagacgc gcggcggcgg
    60421 cagcatcggg accactgttg ttacactgaa gttgggccag attgttcaaa tcctgacgca
    60481 atgcctggta aagtttatcg tctggcagta cgcctgcgtg ggcatcgagc atttcgctgt
    60541 aacgcttgcg ctggctttga taaaccttcg caagcgtagg gtcgtccagc acgcaatact
    60601 gacggtagct acgctccatc tccagcgccg cgttggtcat cgcttcacta cgccgggcat
    60661 caataagcgt agtgcggtta accagcgccg cctgatcgct aagcgcattc aggctttgcc
    60721 aggcttgcca tgccagcacc aacaggggta gtagaattag caaaaatgcc agcattacca
    60781 gttgtcgtaa tgagcgggga aaaacgggcc agcgtttcaa ggtgttactc tcgtcagacg
    60841 cgaatagcct gatgctaacc gaggggaagt tcagatacaa caaagccggg aattacccgg
    60901 ctttgttatg gaataaggcg gtgcctaact cgacgtttcg cccgatggtt gatatagcta
    60961 cgctgatatc agaagttgga cggcaggcac cttgttgtgc gtcattcgaa ttttatgtag
    61021 cacatcccga aggggctgac ataagtcggt gaatgagcca ctgcttacta ttatgcagta
    61081 actgtgccaa taaagaaaat agtttggtaa cgtactgatt atacgttgct ttgagggatt
    61141 tatggcttct tcgctatttg aatgtaaatc agccactgtg tcgctaaaaa gagacaactt
    61201 aagataaact tattagatta caataaaatc aatcatttaa gtgtcgccag aaagcgacac
    61261 ggtaaaccac ccattgtcgt gatttacaga cacaaaaaag ctcccggagt tgggagctta
    61321 tgatagtggt tggtgcttaa tgccggatgc ggcgtaaatg ccgcatccgg gctgcaaaac
    61381 caatgaactg atgacttacc ccaactgctt acgcgcattg cggaaaatgc gcatccatgg
    61441 gccatcctcg ccccagtttt ccggatgcca ggagttgctg acagtacgga aaacacgttc
    61501 cgggtgcggc atcataatgg tgactcgacc actttcagtc gtaaccgacg taataccgtt
    61561 cggagaaccg ttcgggttag ccgggtaggt ttcagtgact ttgccgaagt tatcgacata
    61621 gcgcagtgcc accagccctt tgctttccag tgccgccaga tgcgccgcat cacgcacttc
    61681 cacgcgccct tcaccgtgag agacagcaat cggcatttgc gagcccacca tcccctgcaa
    61741 cagcagcgac gggctttggg tcacttcaac caggctgaaa cgcgcttcaa agcgatcgga
    61801 ggtattgcgc acaaaacgtg gccacaactc actacctggg atcagttcac gcagattaga
    61861 catcatctgg caaccgttac atacccccag cgccagcgtt tgcggacggt ggaagaaggt
    61921 tgcaaactca tcgcgtacac ggtcattgaa caggattgac ttcgcccaac cttcaccggc
    61981 acccagcaca tcaccgtagg agaaaccacc gcacgcgacc agggcgtgga aatcttccag
    62041 gcccgtgcgt ccagccagca ggtcactcat atgcacgtcg atagcatcaa agcctgcacg
    62101 gtggaaagct gccgccattt caacatgcga gttcacgccc tgctcacgca gtacggcaac
    62161 tttcgggcgc gcgccagtgg caataaacgg tgctgccaca tcttcgttga tatcgaacga
    62221 cagttttaca ttcaggcccg gatcggcgtc gtttgatttc gcctggtgct cctggtcagc
    62281 acactccggg ttgtcacgca ggcgctgcat ctgccaggta gtttctgccc accagacacg
    62341 caacgtagtg cggctttcgc tgaatacagt ctgcccgttg gcggtaatca caaaacggtc
    62401 accggaaacc gcctgcccaa cataatggac gcaatcagca agcccatgct gtgccagtac
    62461 ggactcgacc gcttcacggt cagcggcacg aacctgaatc accgcaccca gttcttcgtt
    62521 aaacaacgcc gccaggcgat cgtcacccag agagccgata tccgcatcaa tgccacaatg
    62581 accagcaaag gccatttccg ccagtgtgac caacagaccg ccatcagagc ggtcgtgata
    62641 cgccagcagc ttacgctgtg caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg
    62701 cgcgacatcg cgtacatctg ccggtttgtc gccaagctga cgataaacct gcgccagcgc
    62761 cgttgcgccc agcgcgttat tgcctttgcc caaatcaatc agcagcagtg cgttatcttc
    62821 ggtagaaagc tgcggcgtga tggtgtgacg tacatcttcc acgcgggcaa atgcagaaat
    62881 caccagcgac agcggcgacg tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt
    62941 catcgacata gagtctttac ccaccgggat cgtcaggccc agcgccggac aaagctcttc
    63001 gcccacggct ttaacggctt catacaggcc cgcatcttca ccagggtggc ctgccgccgc
    63061 catccagttg gcggaaagtt taatgcgttt gatatcgcca atctgtgttg cggcaatatt
    63121 ggttaacgct tcaccgaccg ccagacgggc agaggcggcg aaatccagca gcgcaaccgg
    63181 cgcacgctcg ccaatcgcca tcgcttcacc gtagtagctg tcgaggctgg cggtagtgac
    63241 cgcgcagtta gcgaccggca cctgccacgg acccaccatc tgatcgcgcg ctaccatgcc
    63301 agttacgctg cggtcaccga tggtcaccag gaaagttttc tccgccacgg tcggcagatg
    63361 cagtacgcgt ttcaccgcgt ctgcaatggt gatcccttca cggaccagcg catcgccctt
    63421 cgctttcagc gtttgtacat cgcgggtcat cttcggcgtt ttaccaagca ggacgtccag
    63481 cggcagatcg atcggctgat tatcaaaatg acgatcgtgc agagaaagat gcagttcttc
    63541 ggtcgcttca ccaatcaccg cgtagggtgc gcgctcacgc ttacacagtt cgtcaaacag
    63601 cggtaattga tcggcagcaa ccgccagcac gtagcgttcc tgggattcgt tacaccagat
    63661 ttccagcggg ctcatgcccg gttcgtcgct tagaatatcg cgcagttcaa atttaccgcc
    63721 gcgtccgccg tcgctcacca gctccggcat cgcgttagaa agaccgcccg caccgacgtc
    63781 gtggataaac agaattgggt tagcatcacc aagctgccag caacggtcga tcacttcctg
    63841 gcaacgacgc tccatctccg ggttgtcgcg ctgtacggaa gcaaagtcga ggtcggcatc
    63901 agactgacct gacgccatgg aagacgccgc tccgccgcca agaccgatgt tcattgccgg
    63961 gccgccgaga acgaccagct tcgcaccgac gttgatctcg cctttttgta cgtgatcggc
    64021 gcgaatgttg ccgatcccgc ccgccagcat gatcggtttg tgataaccgc gcagctcttc
    64081 gccgttgtgg ctgttcactt tttcttcata agtacggaag tagccattca gtgccggacg
    64141 accaaattcg ttgttaaacg ccgcgccgcc cagcgggcct tcggtcatga tgtccagcgc
    64201 ggtgacaatg cgctcaggct taccgaaatc ttcttcccac ggctgttcga agccaggaat
    64261 tcgcaggttg gaaacggaga aaccaaccaa acccgctttc ggcttcgcgc cgaggccggt
    64321 ggcaccttca tcgcggattt caccgccgga accggtcgcc gcgcccggcc acggagaaat
    64381 cgccgtcggg tggttgtgag tttcgacttt catcagaata tgcgccggtt cctgatggaa
    64441 atcgtagcgg cccgtttcgt ggtcagcaaa gtagcggccc acttcagaac cttccattac
    64501 ggcggcgtta tctttataag cagagagaac gtaatctggc gtggtttcga aggtgttttt
    64561 gatcatctta aacagcgatt tcggctgttg ctcaccgtcg ataacccaat cggcgttaaa
    64621 aatcttgtgg cggcagtgct cggagttcgc ctgggcgaac atatacagct cgatgtcgtt
    64681 cgggttacga ccaagctttg tgaaagcatc ctgcagatag tcaatttcat cttccgccag
    64741 tgccaggcca agacgcaggt tagcgtcgat tagcgcctga cggccctgcc ccagcaaatc
    64801 aacgctggtg accggagtcg gttgatggtg tgcaaacagc tgctctgcat catctaaagc
    64861 aaaaaagacc gtttccatca tgcggtcgtg cagttcagcg gtaacctgct gccattgttc
    64921 attggtcagc gtaccggctt ctatatagta agcaacgccg cgctcaaggc ggtttacctg
    64981 ttgtagcccg cagttatggg caatatcggt cgctttcgaa gaccagggag agatggtgcc
    65041 aggacgcggg gtcaccagca ggagtttgcc ttgcggggcg tggctggcga gtgccgggcc
    65101 atatttcagc aggcgttcaa gttgtgcgtg ctcatcatcg tttaacggcg cattgaggtc
    65161 agcaaaatgg acatactcgg cgtaaatatt gtgaaccggg agcctggcag cctgaaaacg
    65221 tgccagcagt ttgttgattc ggaatgccga cagtgcaggc gaaccacgca gaatttccat
    65281 cataagtctc tcgtcttcta agctttcggt gtacccaagg gggggaaacg ggcgtcatta
    65341 taaagaatct gatgcgctga cgaaaccgtt tgcgtggaaa taaaatcacc atcgtgaatt
    65401 agcaacgcgt gccgccaatg gctgtaataa gttgccatct ggcgcaggtt tacgcaaaat
    65461 gccgctcatt taagagtaaa cctttcacta ttattacgtt ttttcaagct gggacgcgca
    65521 cgacacagag aattaactaa ttgaaaaaat taaagattaa ttatctgttc atcggcattc
    65581 tggcactgct gctcgcggtc gctctctggc catccattcc ctggtttggt aaagccgaca
    65641 accgtatcgc cgccattcaa gcgcggggag agttgcgtgt gagcaccatt catactcccc
    65701 tgacttataa cgaaatcaac gggaaacctt ttggcctgga ttacgaactg gcgaaacagt
    65761 ttgccgatta cctcggcgta aaactgaaag tgaccgtgcg gcagaatatc agccagctgt
    65821 ttgacgacct tgataatggt aacgccgacc tgctggcggc aggactggtc tataacagtg
    65881 agcgggtaaa aaattatcag cctggcccta cctattattc cgtgtcacaa caactggttt
    65941 ataaagtggg tcagtatcgc ccacgtacgc tgggcaacct gacggcggag caactcaccg
    66001 ttgcaccggg tcatgtggtg gttaacgatc tccagaccct gaaagaaaca aaattcccgg
    66061 aattaagctg gaaggtagac gacaaaaaag gctctgcgga attaatggaa gatgtcatcg
    66121 aaggaaaact cgattacacc attgctgatt ctgtcgccat cagcctgttt cagcgcgttc
    66181 acccggagct cgccgtagcg ctcgatatca ccgatgaaca accggtgact tggtttagcc
    66241 cgttagatgg cgataatacc ctttccgccg ccctgctcga cttcttcaac gaaatgaatg
    66301 aagacggtac gctggcacgc attgaagaga aatacctggg gcatggcgat gattttgatt
    66361 acgtcgatac gcgcacattt ttacgcgccg tcgatgcggt actgccgcag ttaaagcccc
    66421 tgtttgagaa atacgccgaa gaaattgact ggcgtttgct ggccgctatt gcttatcagg
    66481 aatcgcactg ggatgcacag gccacttcac cgacgggtgt gcgcggcatg atgatgttaa
    66541 ccaaaaatac cgcgcaaagc ctcggcatta cggatcgtac cgatgccgaa cagagcatca
    66601 gcggtggcgt gcgttatttg caggatatga tgagtaaagt gccggaaagt gtgccggaga
    66661 acgagcggat ctggtttgcc ctcgctgcgt acaatatggg ctatgcgcat atgctggatg
    66721 cccgcgccct gacggcaaaa accaaaggga atcctgacag ttgggctgac gtaaaacagc
    66781 gtctgccttt acttagccag aaaccctatt acagcaagct gacttacggc tacgctcgtg
    66841 gacatgaagc ctacgcttat gtcgaaaata ttcgtaagta tcagattagc ctggtgggtt
    66901 atctgcaaga gaaagagaag caggctacag aagcggcgat gcaactggcg caggattatc
    66961 cggcggtatc gcctacggag ttgggcaaag agaaatttcc ttttctctcg tttctttccc
    67021 agtcgtcatc aaactatttg acccattctc cctctctgct gttttccagg aaagggagtg
    67081 aagagaaaca aaattaatcc gtcgaggatt gcgctttttt ctgcgcttta atttcctggc
    67141 ggcgcatgcg aaagaagtca ctgagcaacg ccgcgcactc atccgccagt attccttccg
    67201 taatttccac tcggtgattc atacccggat gatgcagcac atccattaaa gatcccgcag
    67261 cgccagtttt cgcgtcacgc gcaccaaaga ccacgcgacc aatgcgactg tggatcatcg
    67321 ctccggcaca cattacacat ggttcaagcg tgacatacaa cgtggcgtcg atcagacgat
    67381 aattttgcat caccagacca ccctgccgca gggccatgat ttctgcatgt gcggtgggat
    67441 catggcgacc aatcgggcgg ttccagcctt cgccgattac ccgattgtta tgcactaata
    67501 ccgcgccgac cggcacttcc cgctcatccc aggcacgttt cgccagcgtc agcgcgtgac
    67561 gcatccagta ttcgtggcta aattcgactt cagacaaaaa gaaaactccg gttataaaag
    67621 cgcggcgcat tatacacgga cgctatgctt tactctattc cagttgctgg agttcaccgc
    67681 gcggggttac tcgccaacga tgctgacaga aataaagcag cggattgtcc tgattactgt
    67741 cgctatagcc actgtacagc cgcagcggag tgccgatttt gcgctccagt tgtgcgacct
    67801 tttcatgtcc cagacaacgc atcgtcaata cccaaccacc atagccacgc tgaatttggc
    67861 tggcgataag attaacccgc ggcagccagg gcgtatcgaa ataaaccgct tcaaccagcg
    67921 gctgcggaga gccggtaatc aaccagatat cagcatcgga acttaacagg taggtggtta
    67981 atcgctcctg aaccagcgga aaggcggtaa cattgtcgcg aaaccagcgc acgaaatcgg
    68041 cctgcaacgt ctgtaaacgt gcttcgctgt gaccaaaagt gcacccccac agaagcagac
    68101 tcatcggcca gcgtgccgca cgacctttta tcaataacgc aatggctata atcggtaaca
    68161 acggcaggac aagtaacgca ttcagcggtt ggcgacgcag taaatagcgc agaaaactgc
    68221 cgaacatatc ctgctgatgc aatgttccat ctaagtcaaa aaacaccaca cgacgctcgt
    68281 gagttgccaa accatactcc tctggactga aacatcctga ttaattttcg ctactacata
    68341 gcctaacaga tagatcatca cttttccggc aacaatcgac cgcatcatgg ctaactggaa
    68401 tttttaattc atggcaatta gcggcaatgg aatataaaat tcactcgcgt gtctctcata
    68461 ttgcctgttg tcgccactat ttcatcgcga ggaaagatat gaacggctta cttcgtatcc
    68521 gtcagcgtta ccaggggctt gcccagagtg ataaaaaact ggcggattat ctgctgctac
    68581 aacctgatac ggcgcgccat ttaagctctc agcaactggc caacgaagcc ggagtcagtc
    68641 agtccagcgt cgtgaagttc gcgcaaaaac tcggctataa aggttttccg gcgcttaagt
    68701 tggcgttgag tgaagcgctg gcaagccagc cggaatcgcc ctccgtgccc attcataacc
    68761 aaatccgcgg tgatgatccg ttacggctgg tcggcgaaaa actgattaaa gaaaatactg
    68821 ccgctatgta cgcaacgcta aacgttaata gtgaagagaa actgcatgaa tgcgtaacaa
    68881 tgttgcgctc tgcgcggcgg ataattctga ccggtattgg cgcttcgggt ctggtggcgc
    68941 aaaactttgc ctggaagctg atgaagattg gcttcaatgc tgccgcagtg cgcgatatgc
    69001 atgcgctact cgcaacagta caggcgtcgt cccctgacga tctgttatta gccatttcct
    69061 acaccggtgt acgacgcgag ttaaacctgg cggcagatga gatgctgcga gtgggcggaa
    69121 aagtgctggc gattaccggc tttactccga atgccctgca acagcgtgct tctcattgcc
    69181 tgtataccat tgccgaagaa caagcgacaa acagcgcttc aatctctgct tgtcacgctc
    69241 agggaatgtt aacggatttg ctgttcattg cgctgattca gcaggatctg gaactggcac
    69301 cagaacgtat tcgtcatagt gaagcgctgg tgaaaaaact ggtctgagta aagaatgcgc
    69361 gtataatgcc cgcccggttt gtgttgtttt gagagtttcc ttatggcgtt gttaatcact
    69421 aaaaaatgca tcaattgtga tatgtgtgaa cccgaatgcc cgaatgaggc gatttcaatg
    69481 ggagatcata tctacgagat taacagcgat aagtgtaccg aatgcgtagg gcactacgag
    69541 acaccaacct gccagaaggt gtgcccgatc cccaatacta ttgtgaaaga tccggcgcat
    69601 gtcgagacag aagaacagtt gtgggataaa tttgt
//
LOCUS       DAZGLJ010000004        64597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000004 DAZGLJ010000000
VERSION     DAZGLJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.004"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..77)
                     /locus_tag="ACHK49_000210"
     tRNA            complement(1..77)
                     /locus_tag="ACHK49_000210"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Arg,seq:acg)
     gene            complement(275..351)
                     /locus_tag="ACHK49_000211"
     tRNA            complement(275..351)
                     /locus_tag="ACHK49_000211"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(315..317),aa:Arg,seq:acg)
     gene            complement(355..447)
                     /locus_tag="ACHK49_000212"
     tRNA            complement(355..447)
                     /locus_tag="ACHK49_000212"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(411..413),aa:Ser,seq:gct)
     gene            complement(763..948)
                     /gene="csrA"
                     /locus_tag="ACHK49_000213"
     CDS             complement(763..948)
                     /gene="csrA"
                     /locus_tag="ACHK49_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO9260914.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1183..3813)
                     /gene="alaS"
                     /locus_tag="ACHK49_000214"
     CDS             complement(1183..3813)
                     /gene="alaS"
                     /locus_tag="ACHK49_000214"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO9260915.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3941..4441)
                     /gene="recX"
                     /locus_tag="ACHK49_000215"
     CDS             complement(3941..4441)
                     /gene="recX"
                     /locus_tag="ACHK49_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO9260916.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLVAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4684..5745)
                     /gene="recA"
                     /locus_tag="ACHK49_000216"
     CDS             complement(4684..5745)
                     /gene="recA"
                     /locus_tag="ACHK49_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO9260917.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5825..6322)
                     /gene="pncC"
                     /locus_tag="ACHK49_000217"
     CDS             complement(5825..6322)
                     /gene="pncC"
                     /locus_tag="ACHK49_000217"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO9260918.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6467..7552)
                     /gene="mltB"
                     /locus_tag="ACHK49_000218"
     CDS             complement(6467..7552)
                     /gene="mltB"
                     /locus_tag="ACHK49_000218"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO9260919.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYVQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7808..8371
                     /gene="srlA"
                     /locus_tag="ACHK49_000219"
                     /gene_synonym="gutA"
     CDS             7808..8371
                     /gene="srlA"
                     /locus_tag="ACHK49_000219"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO9260920.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8368..9327
                     /gene="srlE"
                     /locus_tag="ACHK49_000220"
                     /gene_synonym="gutE"
     CDS             8368..9327
                     /gene="srlE"
                     /locus_tag="ACHK49_000220"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO9260921.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFREGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9338..9709
                     /gene="srlB"
                     /locus_tag="ACHK49_000221"
                     /gene_synonym="gutB"
     CDS             9338..9709
                     /gene="srlB"
                     /locus_tag="ACHK49_000221"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO9260922.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9713..10492
                     /gene="srlD"
                     /locus_tag="ACHK49_000222"
     CDS             9713..10492
                     /gene="srlD"
                     /locus_tag="ACHK49_000222"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO9260923.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10597..10956
                     /gene="gutM"
                     /locus_tag="ACHK49_000223"
     CDS             10597..10956
                     /gene="gutM"
                     /locus_tag="ACHK49_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO9260924.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            11023..11796
                     /gene="srlR"
                     /locus_tag="ACHK49_000224"
                     /gene_synonym="gutR"
     CDS             11023..11796
                     /gene="srlR"
                     /locus_tag="ACHK49_000224"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO9260925.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11789..12754
                     /gene="gutQ"
                     /locus_tag="ACHK49_000225"
     CDS             11789..12754
                     /gene="gutQ"
                     /locus_tag="ACHK49_000225"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO9260926.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12751..14265)
                     /gene="norR"
                     /locus_tag="ACHK49_000226"
     CDS             complement(12751..14265)
                     /gene="norR"
                     /locus_tag="ACHK49_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO9260927.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14452..15891
                     /gene="norV"
                     /locus_tag="ACHK49_000227"
     CDS             14452..15891
                     /gene="norV"
                     /locus_tag="ACHK49_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO9260928.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            15888..17021
                     /gene="norW"
                     /locus_tag="ACHK49_000228"
     CDS             15888..17021
                     /gene="norW"
                     /locus_tag="ACHK49_000228"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO9260929.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(17247..19499)
                     /gene="hypF"
                     /locus_tag="ACHK49_000229"
     CDS             complement(17247..19499)
                     /gene="hypF"
                     /locus_tag="ACHK49_000229"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO9260930.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAEFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGKIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19652..20179)
                     /gene="hydN"
                     /locus_tag="ACHK49_000230"
     CDS             complement(19652..20179)
                     /gene="hydN"
                     /locus_tag="ACHK49_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO9260931.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGVGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20328..21338)
                     /gene="ascG"
                     /locus_tag="ACHK49_000231"
     CDS             complement(20328..21338)
                     /gene="ascG"
                     /locus_tag="ACHK49_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO9260932.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGRWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLICRDSLIAL
                     SR"
     gene            21598..23055
                     /gene="ascF"
                     /locus_tag="ACHK49_000232"
     CDS             21598..23055
                     /gene="ascF"
                     /locus_tag="ACHK49_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO9260933.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVMLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            23064..24488
                     /gene="ascB"
                     /locus_tag="ACHK49_000233"
     CDS             23064..24488
                     /gene="ascB"
                     /locus_tag="ACHK49_000233"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO9260934.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSITWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24602..25021)
                     /locus_tag="ACHK49_000234"
     CDS             complement(24602..25021)
                     /locus_tag="ACHK49_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9260935.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(25018..25329)
                     /locus_tag="ACHK49_000235"
     CDS             complement(25018..25329)
                     /locus_tag="ACHK49_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO9260936.1"
                     /translation="MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTA
                     FPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25503..26246)
                     /locus_tag="ACHK49_000236"
     CDS             complement(25503..26246)
                     /locus_tag="ACHK49_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9260937.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(26289..26759)
                     /gene="hycI"
                     /locus_tag="ACHK49_000237"
     CDS             complement(26289..26759)
                     /gene="hycI"
                     /locus_tag="ACHK49_000237"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO9260938.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(26752..27162)
                     /gene="hycH"
                     /locus_tag="ACHK49_000238"
     CDS             complement(26752..27162)
                     /gene="hycH"
                     /locus_tag="ACHK49_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO9260939.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(27159..27926)
                     /gene="hycG"
                     /locus_tag="ACHK49_000239"
     CDS             complement(27159..27926)
                     /gene="hycG"
                     /locus_tag="ACHK49_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO9260940.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(27926..28468)
                     /gene="hycF"
                     /locus_tag="ACHK49_000240"
     CDS             complement(27926..28468)
                     /gene="hycF"
                     /locus_tag="ACHK49_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO9260941.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28478..30187)
                     /gene="hycE"
                     /locus_tag="ACHK49_000241"
     CDS             complement(28478..30187)
                     /gene="hycE"
                     /locus_tag="ACHK49_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO9260942.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(30205..31128)
                     /gene="hycD"
                     /locus_tag="ACHK49_000242"
     CDS             complement(30205..31128)
                     /gene="hycD"
                     /locus_tag="ACHK49_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO9260943.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(31131..32957)
                     /gene="hycC"
                     /locus_tag="ACHK49_000243"
     CDS             complement(31131..32957)
                     /gene="hycC"
                     /locus_tag="ACHK49_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO9260944.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMRVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(32954..33565)
                     /gene="hycB"
                     /locus_tag="ACHK49_000244"
     CDS             complement(32954..33565)
                     /gene="hycB"
                     /locus_tag="ACHK49_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO9260945.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33690..34151)
                     /gene="hycA"
                     /locus_tag="ACHK49_000245"
     CDS             complement(33690..34151)
                     /gene="hycA"
                     /locus_tag="ACHK49_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO9260946.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            34363..34713
                     /gene="hypA"
                     /locus_tag="ACHK49_000246"
     CDS             34363..34713
                     /gene="hypA"
                     /locus_tag="ACHK49_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO9260947.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            34717..35589
                     /gene="hypB"
                     /locus_tag="ACHK49_000247"
     CDS             34717..35589
                     /gene="hypB"
                     /locus_tag="ACHK49_000247"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO9260948.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCLACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35580..35852
                     /gene="hypC"
                     /locus_tag="ACHK49_000248"
     CDS             35580..35852
                     /gene="hypC"
                     /locus_tag="ACHK49_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO9260949.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            35852..36973
                     /gene="hypD"
                     /locus_tag="ACHK49_000249"
     CDS             35852..36973
                     /gene="hypD"
                     /locus_tag="ACHK49_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO9260950.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            36970..37980
                     /gene="hypE"
                     /locus_tag="ACHK49_000250"
     CDS             36970..37980
                     /gene="hypE"
                     /locus_tag="ACHK49_000250"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO9260951.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            38054..40131
                     /gene="flhA"
                     /locus_tag="ACHK49_000251"
                     /pseudo
     CDS             38054..40131
                     /gene="flhA"
                     /locus_tag="ACHK49_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(40168..40521)
                     /gene="ygbA"
                     /locus_tag="ACHK49_000252"
     CDS             complement(40168..40521)
                     /gene="ygbA"
                     /locus_tag="ACHK49_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO9260952.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(40598..40732)
                     /locus_tag="ACHK49_000253"
     CDS             complement(40598..40732)
                     /locus_tag="ACHK49_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9260953.1"
                     /translation="MIPELIISGEEANCEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            40808..43369
                     /gene="mutS"
                     /locus_tag="ACHK49_000254"
     CDS             40808..43369
                     /gene="mutS"
                     /locus_tag="ACHK49_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO9260954.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            43475..44131
                     /gene="pphB"
                     /locus_tag="ACHK49_000255"
     CDS             43475..44131
                     /gene="pphB"
                     /locus_tag="ACHK49_000255"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO9260955.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(44182..44949)
                     /gene="ygbI"
                     /locus_tag="ACHK49_000256"
     CDS             complement(44182..44949)
                     /gene="ygbI"
                     /locus_tag="ACHK49_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO9260956.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            45145..46053
                     /gene="ltnD"
                     /locus_tag="ACHK49_000257"
     CDS             45145..46053
                     /gene="ltnD"
                     /locus_tag="ACHK49_000257"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO9260957.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            46050..47312
                     /gene="otnK"
                     /locus_tag="ACHK49_000258"
     CDS             46050..47312
                     /gene="otnK"
                     /locus_tag="ACHK49_000258"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO9260958.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            47309..47947
                     /gene="ygbL"
                     /locus_tag="ACHK49_000259"
     CDS             47309..47947
                     /gene="ygbL"
                     /locus_tag="ACHK49_000259"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO9260959.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            47952..48728
                     /gene="ygbM"
                     /locus_tag="ACHK49_000260"
     CDS             47952..48728
                     /gene="ygbM"
                     /locus_tag="ACHK49_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO9260960.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            48817..50181
                     /gene="ygbN"
                     /locus_tag="ACHK49_000261"
     CDS             48817..50181
                     /gene="ygbN"
                     /locus_tag="ACHK49_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO9260961.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(50220..50456)
                     /locus_tag="ACHK49_000262"
     CDS             complement(50220..50456)
                     /locus_tag="ACHK49_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO9260962.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTESLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(50467..51894)
                     /locus_tag="ACHK49_000263"
     CDS             complement(50467..51894)
                     /locus_tag="ACHK49_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO9260963.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(51894..52487)
                     /locus_tag="ACHK49_000264"
     CDS             complement(51894..52487)
                     /locus_tag="ACHK49_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO9260964.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            52634..53041
                     /locus_tag="ACHK49_000265"
     CDS             52634..53041
                     /locus_tag="ACHK49_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO9260965.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(53162..54154)
                     /gene="rpoS"
                     /locus_tag="ACHK49_000266"
     CDS             complement(53162..54154)
                     /gene="rpoS"
                     /locus_tag="ACHK49_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO9260966.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(54217..55311)
                     /gene="nlpD"
                     /locus_tag="ACHK49_000267"
     CDS             complement(54217..55311)
                     /gene="nlpD"
                     /locus_tag="ACHK49_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO9260967.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(55496..56122)
                     /gene="pcm"
                     /locus_tag="ACHK49_000268"
     CDS             complement(55496..56122)
                     /gene="pcm"
                     /locus_tag="ACHK49_000268"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO9260968.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(56116..56877)
                     /gene="surE"
                     /locus_tag="ACHK49_000269"
     CDS             complement(56116..56877)
                     /gene="surE"
                     /locus_tag="ACHK49_000269"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO9260969.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(56858..57907)
                     /gene="truD"
                     /locus_tag="ACHK49_000270"
     CDS             complement(56858..57907)
                     /gene="truD"
                     /locus_tag="ACHK49_000270"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO9260970.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLNDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(57904..58383)
                     /gene="ispF"
                     /locus_tag="ACHK49_000271"
     CDS             complement(57904..58383)
                     /gene="ispF"
                     /locus_tag="ACHK49_000271"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO9260971.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(58383..59093)
                     /gene="ispD"
                     /locus_tag="ACHK49_000272"
     CDS             complement(58383..59093)
                     /gene="ispD"
                     /locus_tag="ACHK49_000272"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO9260972.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(59112..59423)
                     /gene="ftsB"
                     /locus_tag="ACHK49_000273"
     CDS             complement(59112..59423)
                     /gene="ftsB"
                     /locus_tag="ACHK49_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO9260973.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(59617..59940)
                     /gene="ygbE"
                     /locus_tag="ACHK49_000274"
     CDS             complement(59617..59940)
                     /gene="ygbE"
                     /locus_tag="ACHK49_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO9260974.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(59990..60595)
                     /gene="cysC"
                     /locus_tag="ACHK49_000275"
     CDS             complement(59990..60595)
                     /gene="cysC"
                     /locus_tag="ACHK49_000275"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO9260975.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDTDRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(60595..62022)
                     /gene="cysN"
                     /locus_tag="ACHK49_000276"
     CDS             complement(60595..62022)
                     /gene="cysN"
                     /locus_tag="ACHK49_000276"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO9260976.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGS
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(62024..62932)
                     /gene="cysD"
                     /locus_tag="ACHK49_000277"
     CDS             complement(62024..62932)
                     /gene="cysD"
                     /locus_tag="ACHK49_000277"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO9260977.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            63184..64221
                     /gene="iap"
                     /locus_tag="ACHK49_000278"
     CDS             63184..64221
                     /gene="iap"
                     /locus_tag="ACHK49_000278"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO9260978.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   64303..64575
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=64364..64392
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
BASE COUNT        15180 a        17663 c        16742 g        15012 t
ORIGIN      
        1 tggtgcatcc gggaggattc gaacctccga ccgctcggtt cgtagccgag tactctatcc
       61 agctgagcta cggatgcatc gggaaactta ctttactgca gattttttga taccgctact
      121 aaagccgtat caagtaagag atggtgcatc cgggaggatt actcgccttg cggctcgccc
      181 ttcgggccgt tgctaaagca acgttatcct ccctggtgcc tgcggttata ccgccacctc
      241 ttccatacct tcatcgctga aagtacgtag aatatggtgc atccgggagg attcgaacct
      301 ccgaccgctc ggttcgtagc cgagtactct atccagctga gctacggatg caaatggcgg
      361 tgaggcgggg attcgaaccc cggatgcagc ttttgaccgc atactccctt agcaggggag
      421 cgccttcagc ctctcggcca cctcaccaca cgcctcttac gagtgcttcg aagaacttgt
      481 ttatcgctca tcgtcgctgc gtggcgcaca tattactttc tgagacttat aagtcaaaca
      541 atttttccta cacttttatc gtttgcacac ttcacgttca attagtctgt aaaaacgtca
      601 aaaagagtgt tttatcaaca gaaaaatgga ggtctgacag atagtagtaa tgcaaaaaaa
      661 tggagactta agttgaatga acgggagtaa agcgaaaaga ctatagagtg aaggagaaat
      721 tttgagggtg cgtctcaccg ataaagatga gacgcggaaa gattagtaac tggactgctg
      781 ggatttttca gcctggatac gctggtagat ctcttcacgg tgaacagaaa cttccttcgg
      841 ggcatttacg ccaatacgta cctggttgcc ctttacccct aaaactgtca cggtgacctc
      901 atccccaatc atgagggtct caccaactcg acgagtcaga atcagcattc tttgctcctt
      961 gaaagattaa aagagtcggg tctctctgta tcccggcatt atccatcata taacgccaaa
     1021 aagtaagcaa tgacaaacac attacatgta agcagtcatg gcattacatt ctgttaaacc
     1081 taagtttagc cgatatacac aacttcaacc tgactttatc gttgtcgata gcgttaatgc
     1141 gaatgccgtg aagcgagtcc acggcattgc ctgacgctta tattattgca atttcgcgct
     1201 gacccagcct ttcacactgg ctaacgctgc aggtaaggcc gcagcatccg taccaccggc
     1261 ttgcgccatg tcaggacgtc caccaccctt gccgcccacc tgctgagcga ccataccaat
     1321 cagttcccct gctttcacac gatctgtgac gtccttagat acgcctgcaa tcagagaaac
     1381 cttaccttcg gctaccgttg ccagcacgat aattgtcgac cccagctgat tttttaaatc
     1441 gtcaaccatg gtacgcaaca ttttcggctc aacaccgcta agctcgctaa ccaacagctt
     1501 aacaccatta acatcaattg ccttactgga aagatttgcg ctctcctgtg cggcagcttg
     1561 ttctttaagc tgttgtagtt ctttttctaa ctgacgcgta cgttccagta ctgagcgcac
     1621 tttatccgcc agattattgc tatcgccttt cagcagatgc gcgacttcgc ttaagcgatc
     1681 gctgtctgca tgaacggtgg tgatagcgcc ttctccggtt accgcttcga tacgacgaac
     1741 gcctgcagca gtacccgatt cagagatgat gcggaacaga ccaatatcac cagtgcggct
     1801 ggcgtgagta ccgccacaca gctcggtgga gaaatcgccc atgctcagca cgcgtacacg
     1861 ctcatcatac ttctcgccga acagcgccat cgcacctttc gctttcgccg cttcgagatc
     1921 catgatgttg gtttcgatcg gcaaattgcg acgaatctgt gcgttcacca ggtcttcgac
     1981 cgcacgaatc tcttccggtt tcatcgcttc gttgtgtgag aagtcgaagc gcagcacctt
     2041 gtcgttaacc agtgaacctt tctgcgatac atgagtcccc agaacctggc gcagcgcagc
     2101 gtgcatcagg tgcgttgcgg agtgattcag acgaatacgg gcgcgacgag cctcatcaac
     2161 atcagcctgc acagcgtcgc ccactttcag agaacccgca gcaagtttac cgatgtgacc
     2221 aatcgcctgg ccgtatttct gcgtatcttc caccgcgaag gagaagttag cgcctttcag
     2281 ttcgccttta tcaccaacct gaccgccgga ttccgcatag aatggtgttt gatccagcac
     2341 gaccacagct tcctggcctg cattgattgc atcaaccgct ttaccatcaa caaacagcgc
     2401 ggtcactttg ccgttcagtt ccagatggtc atagccttta aattcagatg cactatcaac
     2461 acggatcatt gcgttgtaat cggcaccaaa gccgctggct tcgcgcgcgc gacgacgctg
     2521 ctcttccatt gctgcttcaa aaccagcttc gtcaactttg atgttgcgct cacgacaaac
     2581 atcagccgtc aggtcaaccg ggaagccata ggtgtcgtac agacggaaag ccgtttcacc
     2641 atccagcgta tcaccagaaa gttttgccag ctcttcatcc agcaacgcca gaccgcgctc
     2701 cagagtacgg gcaaactgct cttcttcagt cttcagcacc tgctcaacct gcgcctgctg
     2761 gcgtttcagg tcttcacccg cagagcccat aacgtcgatc agcggaccaa ccagtttgta
     2821 gaagaaggtt tctttcgcgc cgagcatatt gccgtgacgc actgcgcgac gaatgatacg
     2881 acgcagtaca taaccacggt tttcattgga cggcattacg ccatccgcga tcaggaacgc
     2941 acaagaacga atgtggtcag cgattacgcg cagcgattta ttgctcagat cggtcgcgcc
     3001 agtgactttc gctaccgcct ggatcaacgt gcggaacagg tcgatgtcat agttagagtt
     3061 aacgtgttgc agcaccgcag caatacgctc cagacccata ccggtatcta cagacggctt
     3121 cggcagcggt tccatcgtgc catctgcctg gcggttgaac tgcatgaaga cgatgttcca
     3181 gatctcaatg tagcggtcgc cgtcttcttc cggacttccc ggaggaccac cccaaatgtg
     3241 gtcgccgtga tcgtagaaga tttcggtgca cgggccgcac ggaccagtgt cacccatctg
     3301 ccagaagttg tcagatgcgt atggcgcacc tttgttatcg ccgatgcgaa taatacgttc
     3361 gcgcgggatc ccgacttctt tttcccagat ttcgtaggct tcgtcgtcgc tttcatagac
     3421 ggtaacccac agacgctctt tcggcagggc aaaccatttt tcgctggtca gcagttccca
     3481 tgcaaactga atggcatcgt gtttgaaata gtcgccgaag ctgaagttgc ccagcatttc
     3541 gaagaaggta tggtgacgcg cggtgtaacc gacgttttcc aggtcgttgt gtttaccacc
     3601 cgcacgcacg cagcgttggg aagtggtagc gcgggaataa ttacgcttgt cgagcccaag
     3661 gaacacatcc ttgaactggt tcatcccggc gttggtaaac aacaaagttg ggtcgttatg
     3721 gggtaccagg gagctgctgg caactacctg atgtccctta ctatggaaaa agtcgagaaa
     3781 cgcctgacgg atctcagcgg tgctcttgct cataattatc ctgaaatcaa gctaacgaaa
     3841 tatcgccacc agctccagcg tgtcttaacc gccgggctgg taactgaaaa gtgggaataa
     3901 gataagtttt cttgactggg aagtaaaata ccgtatgcgt tcagtcggca aaatttcgcc
     3961 aaatatcctg gatatcttcc atcagatagc cacgatagag cagaaaacgc tggatcttaa
     4021 ctttttctga aaagacagtt ggcaaaggtt caccatattt tcgcgtcgcc tgatcgcgcg
     4081 ccagtgcgca ccagtcgatg tcacattcac gcatcgcttt ttctgtcgct tcgcgggaaa
     4141 tacctttctg attcagttcc tgacgaatac gtgcaggtcc ataacctttg cggctacggc
     4201 tggcgataaa gcgcgcaaca aatcggctgt catcgagata gccatgttca tggcaccagg
     4261 caataacgcg ctcgtaatct tctgccgtag catcaatctc ttctgggcca tttttgccca
     4321 taatcggtgc cacgagttta cgtcgcagtt cttgctcact gtgatcgcgc accgccagaa
     4381 tgcgtaccgc acgatccaac aggcgagcat atgccgggcg acgggacgtt gattctgtca
     4441 tggcatatcc ttacaactta aaaaagcaaa agggccgcag atgcgaccca ggttttgctg
     4501 ataaacagat ttaatagtca atgagtgtgc agaatgttcc cgcaggccca attcgttaga
     4561 ggatcgaggt gagagtaatt tttcacgcca gtgctggcct gttcccctcc ccctaaccct
     4621 ctccccaaag gggcgagggg accgtccgtg cacacattat ctattatcat caataacgaa
     4681 cgattaaaaa tcttcgttag tttctgctac gccttcgctg tcatctacag agaaatccgg
     4741 cgttgagttc gggttgctca gcagcaactc acgtactttc ttctcgatct ctttcgcggt
     4801 ttccgggtta tctttcagcc aggcagttgc attcgcttta ccctgaccga tcttctcacc
     4861 tttgtagctg taccacgcgc ctgctttctc gatcagcttc tcttttacgc ccaggtcaac
     4921 cagttcgccg tagaagttga taccttcgcc gtagaggatc tgaaattcag cctgtttaaa
     4981 cggtgcagcg attttgttct tcaccacttt cacgcgggtt tcgctaccca ccacgttttc
     5041 gccctctttc accgcgccga tacgacggat gtcgagacga acagaggcgt agaatttcag
     5101 cgcgttacca ccggtagtgg tttccgggtt accgaacatc acaccaattt tcatacggat
     5161 ctggttgatg aagatcagca gcgtgttgga ctgcttcagg ttacccgcca gcttacgcat
     5221 cgcctggctc atcatacgtg ccgcaaggcc catgtgagag tcgccgattt cgccttcgat
     5281 ttccgctttc ggcgtcagtg ccgccacgga gtcaacgacg ataacgtcta ctgcgccaga
     5341 acgcgccagg gcgtcacaga tttccagtgc ctgctcgccg gtgtccggct gggagcacag
     5401 caggttatcg atatcgacgc ccagtttacg tgcgtagatt gggtccagcg cgtgttcagc
     5461 atcgataaac gcacaggttt taccttcacg ctgcgctgcg gcgatcacct gcagcgtcag
     5521 cgtggtttta ccggaagatt ccggtccgta gatttcgacg atacggccca tcggcagacc
     5581 acctgcccca agcgcgatat ccagtgaaag cgaaccggta gagatggttt ccacatccat
     5641 ggaacggtct tcacccaggc gcatgatgga gcctttacca aattgtttct caatctggcc
     5701 cagtgctgcc gccaacgctt tctgtttgtt ttcgtcgata gccattttta ctcctgtcat
     5761 gccgggtaat accggatagt caatatgttc tgttgaagca attatactgt atgctcatac
     5821 agtatcaagt gttttgtaga aattgttgcc acaaggtctg caatgcatac gcagtagcct
     5881 gacgacgcac cgcatcacgg tcaccgctga agcattcccg ccgggtaatg ccttcaccgc
     5941 gggcagtggc aaaagcaaac cagacggtgc cgacaggctt ctcttcactg ccgccatccg
     6001 gcccggcgat accactaata gacacggcat aatcagcacg agccgctttc agtgcaccta
     6061 tcgccatttc caccacgacg ggttcactca ccgcgccatg ctgcgccagc gtctcttcgc
     6121 gtacgccgat catctgcgct ttggcttcgt tactgtaggt gacaaatccg cgttcaaacc
     6181 aggcggagct accggcaata tcggtaatca ctttcgctac ccaaccaccg gtacaagact
     6241 cggcagttgt tacggttgcg ccacgggctt tcagcgcctg cccaacctgt tcacttaact
     6301 gcatcagttc actgtcagtc atcacaacct ctgtcagtca aaaatttatg ccggacaaga
     6361 tagcactttc gtcaggaaga tggtgttggc gttgcaattt tacgaggagg attcagaaaa
     6421 aagctgatta gccagaggga agctcacgcc cccctcttgc aaatagttac tgcacgcgcg
     6481 ccagcgccac ggcttgtcct aactgccaga ccgccattgc gtaatgggtg ctgtggttat
     6541 aacgggtaat ggtgtagaag ttcggcagac cgtaccagta ctggtaaccg gtaccgacat
     6601 ccagacgcag caggctggct tgttgatggt tgcccagcgg ctgctgtggc gttaaacctg
     6661 ccgtggcaag ctgcgaaatg ctgtacctgg ttttgaagcc atttggcaag cctggagcct
     6721 gaccgtttgc cattaccgcg acctgatcgc ctttcaccca gccgtgcgct ttgaaatagt
     6781 tcgccacgct accgatcgca tcaaccggat cccacaggtt gatatgcccg tcgccgctga
     6841 aatctaccgc atattgtttg taagacgacg gcataaactg tacgtagccc atcgccccgg
     6901 caaaggaacc tttcagattg agcggatcgt cctgctcgtc gcgcgccatc aacaggaagg
     6961 tttccagctc gccagagaaa tactccgcgc ggcgtgggta gttaaatgac agcgttgcca
     7021 gcgcatcgag gatgcgagtt ttccccatca cgcgccccca gcgggtttca acgccgataa
     7081 tcccgacgat aatttccggc ggtacgccat acacctgcca cgcgcgattc aacgcatctt
     7141 catactgatt ccagaaaacc acaccgttct gcacgttgtc cggcgtaata aattttttgc
     7201 gataacggag ccatgcgccg ttcggacctg atggcggttt caccgatgtg gttggtgcct
     7261 ggttatccat cagccgcagt accgaatcca gacgcttcgc ctgggagaga atttcctgca
     7321 actgctgacg atcgaaaccg tgtttgttca ccattttgtc gatgaactgc tgggcattcg
     7381 ggttattagc gaaatcgccg cccatctgca tcacattgtg ctgcggctca agcaggaagc
     7441 cgccagacgg cgttccggtg gtcgtttcag tctcagtagg ttttggcttg ctgctacagg
     7501 cggcaagcaa cacaaaaagg ggaagcaatg ttacataacg acgcttgaac atgaggggtc
     7561 catttaacag attcaaccag gggcaagtat ggtaaagcat cacgccccac acaaggaagc
     7621 ggtagtcact gcccgatacg gactttacat aactcaactc attcccctcg ctatcctttt
     7681 actcaaactt tcaaattaaa atatttatct ttcattttga gatcaaaata acacttttaa
     7741 atctttcaat ctgattagat taggttgccg tttggtaata aaacaataaa tcctgaagga
     7801 gagaacaatg atagaaacca ttactcatgg tgcagagtgg tttatcgggc tgttccaaaa
     7861 gggcggagag gtgtttaccg ggatggtgac cggcattctt ccgctgttga ttagcctgct
     7921 ggttatcatg aacgcactga ttaattttat cggtcagcat cgtattgaac gttttgctca
     7981 acgttgcgcc ggtaaccctg tttcccgtta cctactgtta ccgtgcattg gcacgtttgt
     8041 cttttgcaat ccgatgacct taagcctggg tcgctttatg ccggaaaagt acaaacccag
     8101 ctactacgcg gcggcctctt atagctgcca ctcaatgaat ggcctcttcc cccatatcaa
     8161 ccctggcgaa ctgtttgttt atcttggcat tgccagcggt ctgacaacgc tgaacctgcc
     8221 acttggccca ctggcggtga gttatctgct ggttggtctg gtcaccaatt tcttccgcgg
     8281 ctgggtgacc gatctgacca ccgccatttt tgagaaaaag atgggcattc aacttgaaca
     8341 aaaagttcac ctggcaggag caacatcatg acgcatattc ggatcgaaaa aggaacgggt
     8401 ggctggggcg gcccgcttga gctgaaagcc acgccgggaa aaaaaatcgt ctatatcacc
     8461 gccggtaccc ggcctgcgat tgttgacaaa ctggcacagc ttactggctg gcaggctatt
     8521 gacggattta gagaaggtga acccgcggag gcggaaattg gtgtcgcggt aatcgactgt
     8581 ggcggcacat tacgctgcgg catctatccg aaacgacgta ttcccaccat taatatccac
     8641 tcgacgggca agtccggtcc gctggcgcag tacattgtgg aagatattta tgtctctggc
     8701 gtaaaagaag aaaacatcac tgtagtgggt gatgcgacac cacaaccctc ttccgtgggc
     8761 cgtgactatg acaccagtaa gaaaatcacc gaacaaagcg atggtttact ggcgaaggtg
     8821 ggaatgggca tggggtccgc cgttgcggtg ctgtttcaat ctggtcgtga caccatcgac
     8881 actgtattaa aaaccattct gccgtttatg gcattcgtct cggcgctcat tggcatcatt
     8941 atggcttctg gccttggtga ctggattgcc cacggtcttg ctccgctggc gagccatcca
     9001 ctgggtctgg tcatgctggc gctcatctgc tccttcccac tgctttcacc tttcctcggc
     9061 ccaggcgcag ttatcgcaca ggttatcggc gtattgattg gcgtgcagat tggtctcggc
     9121 aatattccgc cgcatctggc tttaccggca ctgtttgcca tcaacgcgca ggcggcctgc
     9181 gacttcatcc cggtcggttt gtcgctggcg gaagcccgtc aggacacggt tcgcgtcggt
     9241 gtcccttctg tactggtgag ccgcttttta accggcgcac cgactgtact gatcgcctgg
     9301 tttgtctccg gttttatcta tcaatagagg ctgaaacatg accgttattt atcagaccac
     9361 catcacccgt atcggcgcga gtgccactga cgccctcagc gaccagatgc tcatcacctt
     9421 tcgtgaaggc gcgccagcgg acctcgaaga gtattgcttc attcattgcc acggcgagtt
     9481 gaaaggtgca ctccatcccg gtttgcaatt tacgctcggg cagcatcgct atccggtgac
     9541 cgctgttggc agcgtggcgg aagacaacct tcgcgaactg ggtcatgtca ccctgcgctt
     9601 cgatggttta aacgaagcgg aatttccggg cactgtccat gtggcaggcc ctgtacccga
     9661 cgatatcgcg ccgggatcgg ttttgaagtt tgaatctgtt aaggagtaaa aaatgaatca
     9721 ggttgccgtt gtcatcggtg gtgggcaaac cttaggcgcg ttcctgtgcc acggtctggc
     9781 tgccgagggg tatcgcgtcg cggttgtcga tattcagagc gacaaagccg caaatgtggc
     9841 acaagaaatt aacgccgaat atggtgaaag tatggcgtac ggttttggtg ctgacgccac
     9901 tagcgagcaa agcgttctgg cgctctctcg tggggtagat gaaatctttg gtcgcgtgga
     9961 tttgctggtc tatagcgccg gaatagcaaa agcagccttt atcagcgact tccagctcgg
    10021 cgattttgac cgttcgctac aagtgaatct ggtgggttat ttcctgtgtg cccgtgaatt
    10081 ttcgcgtttg atgatccgcg acgggattca gggtcgcatt attcagatca actcgaaatc
    10141 cggcaaagtg ggcagcaaac acaactctgg ctacagcgca gcgaaatttg gtggcgtcgg
    10201 gctgactcaa tcactggcgc tggatctggc ggagtacggc attacggtgc attcactgat
    10261 gctcggtaac ctgctgaaat cgccgatgtt ccagtcactg ttgccacaat acgcgaccaa
    10321 gctgggtatc aaaccggatc aagtcgagca gtattacatc gacaaagtac cgctcaaacg
    10381 cggctgcgat tatcaagatg tgctgaatat gctgctgttc tacgccagtc ctaaggcgtc
    10441 gtactgcacc ggacagtcga tcaatgtcac cggcggtcag gtgatgttct gatcaacagc
    10501 ggagatccat taaggatctc cgtgagacta tagaatgcct gatgcgctac gctcatcagg
    10561 catacaggac ttccgccact acattaagga aaagttatgg tatccgcact catcaccgtc
    10621 gccgttatcg cctggtgtgc gcaactggcc ttaggcggct ggcaaatttc tcgttttaac
    10681 cgtgccttcg acacactatg ccagcaaggg cgggttggcg tgggccgttc cagcgggcgc
    10741 tttaaaccgc gggtcgtggt cgccatcgcg ctggacgatc agcagcgcat cgtcgacacc
    10801 ttgtttatga aaggactgac cgtcttcgcc cgaccgcaaa aaattcccgc aattaccggt
    10861 atgcatgcgg gtgatttaca gcccgatgtg atctttcccc atgatccact atcacagaat
    10921 gctctatcat tggcgcttaa actgaaacgt ggataatttc gttgtgaatg ttacttgctt
    10981 gcgaagttat cattttgaaa cctaaatcag gtaatcacgc ccatgaaacc tcgtcagcgt
    11041 caggccgcca ttctggagta tctgcaaaag cagggtaaat gctcggttga agaattggcg
    11101 caatactttg acaccacagg cacaaccatt cgcaaagatc tggtcattct ggaacatgcc
    11161 ggaaccgtca ttcgtactta tggcggagtg gtgttgaata aagaggaatc cgatccgcct
    11221 atcgatcata aaacactcat caacacccac aagaaagagc tgattgcaga agctgccgtt
    11281 agttttatcc atgatggcga ttcgatcatt cttgatgctg gcagtaccgt tttgcagatg
    11341 gttcccctgc tctcgcgctt taataacatc acggtgatga ccaacagcct gcatatcgtc
    11401 aatgcgctat ccgaactgga taacgaacaa actatcctga tgccaggcgg aacgtttcgt
    11461 aaaaaatcgg cctcatttca cggacagctg gcagagaatg ccttcgaaca tttcagtttc
    11521 gataaattgt ttatgggcac cgacggcatc gatctcaatg cgggcgtaac caccttcaac
    11581 gaggtttata ccgtcagtaa ggcaatgtgc aatgccgcgc gcgaagtgat tttgatggcg
    11641 gactcatcaa agtttggccg taaaagcccc aacgtagttt gcagtcttga aagcgtcgat
    11701 aagctgatta ccgacgcagg tatcgatccg gcgtttcgtc aggcgctgga agagaaaggg
    11761 atcgatgtga tcataaccgg agagagcaat gagtgaagca ctactgaacg cgggacgtca
    11821 gacgttaatg ctggagttgc aggaagcaag ccgtttaccg gaacgtctgg gcgatgattt
    11881 tgttcgcgcc gccaatatca tcctgcactg cgaaggcaaa gtggtggttt cgggaattgg
    11941 caaatcgggc cacattggta agaaaatcgc cgcaacactt gccagtaccg gcacaccggc
    12001 tttttttgtc catccggcag aagcactgca cggcgatctg gggatgattg aaagccgcga
    12061 tgtgatgctg tttatctctt actccggtgg cgcgaaggaa ctggatctga ttattccgcg
    12121 tctggaagat aaatctatcg cgctgctggc gatgaccggc aaaccgacgt caccgctggg
    12181 cctggcggca aaagcggtgc tggatatctc cgtagaacgc gaagcctgcc cgatgcacct
    12241 tgcgccgacc tccagcaccg tcaataccct gatgatgggt gacgcgctgg cgatggcggt
    12301 catgcaggcg cgcggattta atgaagaaga ttttgcccgc tcccacccag ccggggcact
    12361 gggcgctcgc ttgctgaata aagtgcatca tctgatgcgc cgtgacgatg ccatcccaca
    12421 ggtggcgtta accgccagcg tgatggatgc gatgctggaa ctcagccgca ccggtctggg
    12481 gctggtggcg gtatgtgacg ctcaacaaca ggtacaaggc gtctttaccg acggcgattt
    12541 acgtcgctgg ctggttggcg gcggcgcact caccacgcca gttaatgaag cgatgacgac
    12601 aggcggcacc acattacagg cacaaagtcg cgccatcgac gccaaagaga tcctgatgaa
    12661 gcgcaaaatc actgccgcac cggtggtgga tgaaaacggc aaactcaccg gcgcaattaa
    12721 cctgcaggat ttctatcagg ccgggattat ttaatccttc aatcccagac gtttcgccag
    12781 ccgatgcagg ttggcgacgt cggtttccag catccgcgcg caggcagccc agttgtgatg
    12841 attttgtgcc agtgcctggc gaatggtttc acgctggaac gcttctgtcg cttcacgcag
    12901 gttttgctta acaacgggca ccgccgccgc ttctggcggc ggcaacgtca cctcaggaaa
    12961 agcaaaatgt tgtgcctcaa gaatcacttc atcgccgctg cgagtggctc tcgccagcac
    13021 taccgcccga tgaatagcat gttccagttc gcgcacgttc cccggaaaac gatagtgttg
    13081 cagtaaattt cgcgctccgg cacttaatac cacgcgggag agccccagcc gcaaacgaca
    13141 ctgctcgcag aaataccccg ccagcagaat gacatcatcg ccccgctcac gcagcggcgg
    13201 caccgaaagt ggaaacacgc tcaggcgatg aaacaaatcg gcgcggaatc gccctgccag
    13261 cacctcttcg cgtaagtcgc ggttagtcgc cgccagcacg cgcacatcga cccgcaaact
    13321 acggtcatcg ccaacgcgct gaatatcgcc atactgcaac accctcagca gcttggcctg
    13381 caatgccaac gacaactcgc cgatctcatc gagaaacaga gtgccgttat ccgccatttc
    13441 aaacttcccg ctgcggttac tgatagcgcc agtaaacgct cctttcacat gcccgaacaa
    13501 ctcactttcc gccacacttt ccggcagtgc ggcacagttg agatagacca gcggattcac
    13561 cgcccgtggc gaggcttcat gaatcgcttt cgccaccagc tccttaccgg ttcccgtctc
    13621 accgctgatc agaacgttga gatcggacgc cgccacaatc tcaatctctt ttttcaattg
    13681 cgtcatgcct ggcgacaagc caatcatctg cgtctgtttc accgcttcaa acggcgcggc
    13741 atcgcccggc agcatattct ggctttccag ttgttcaatc agcaacgcat tgcttaacgc
    13801 tcccgccgcc agcgcggcaa tcagccgtaa ctcttcgtcg ctgaaaacat cgaactgatc
    13861 gggctgcatc ccgtcgagcg tcagtgcgcc gatcaggttt tgcccggcaa acaatggcag
    13921 accaacgcag gcgtgaacct tcagactctc ctgcccagga atcaaaccgt catagggatc
    13981 gggcaattcg ctgtctgcag gaaagcgcac cacgtccccg gcgcgggcaa tcgcttcaag
    14041 ccgtggatgc ccttccagcg caaagcgtct accgagtaca tcctttgcca gaccgtcgat
    14101 ggcaagcgga ataaactgcc gcgaatcgta acgtagcaac gcagacgcat cgcactccag
    14161 cacctgacgt agcgtggtga tcaggcgctg aaaacgatcc tgatgaccaa tcccacgctg
    14221 caattcgatg gcgatattcg ccagcacatc aacggaaaaa ctcatctttg cctcactgtc
    14281 aatttgacta tagatattgt catatcgacc atttgattga tagtcatttt gactactcat
    14341 taatgagcat aattttattt atagagtaaa aacaatcaga taaaaaactg gcacgcaatc
    14401 tgcaattagc aagacatctt tttagaacac gctgaataaa ttgaggttgc tatgtctatt
    14461 gtggtgaaaa ataacattca ttgggttggt caacgtgact gggaagtgcg tgattttcac
    14521 ggcacggaat ataaaacgct gcgcggcagc agttacaaca gctacctcat ccgcgaagaa
    14581 aaaaacgtgc tgatcgacac cgtcgaccat aaattcagcc gcgaatttgt gcagaacctg
    14641 cgtaatgaaa tcgatctggc ggatatcgat tacatcgtga ttaaccatgc agaagaggac
    14701 cacgctgggg cgctgaccga actgatggcg caaattcccg atacgccgat ctactgtact
    14761 gccaacgcta tcgactcgat aaatggtcat caccatcatc cggagtggaa ttttaatgtg
    14821 gtgaaaactg gcgacacgct ggatatcggc aacggcaaac agctcatttt tgtcgaaaca
    14881 ccaatgctgc actggccgga cagcatgatg acttacctga caggcgacgc ggtgctgttc
    14941 agtaacgatg ctttcggtca gcactactgc gacgagcatc tgttcaacga tgaagtggat
    15001 cagacggagc ttttcgagca gtgccagcgt tactacgcca atatcctgac gccgttcagc
    15061 cgcctggtaa cgccgaaaat taccgagatc ctgggcttta acttaccagt cgatatgata
    15121 gcgacctccc acggcgtggt ctggcgcgat aacccgacgc aaattgtcga gctgtacctg
    15181 aagtgggccg ctgattatca ggaagacaga atcaccattt tctacgacac catgtcgaat
    15241 aacacccgca tgatggctga cgctatcgcc cagggaattg cggaaaccga cccacgcgtg
    15301 gcggtgaaaa ttttcaacgt cgcccgaagc gataaaaacg aaatcctgac caatgtcttc
    15361 cgctcaaaag gcgtgctggt cggcacttcg acgatgaata acgtgatgat gccgaaaatc
    15421 gccgggctgg tggaggagat gactggttta cgcttccgta acaaacgcgc cagtgctttc
    15481 ggctctcacg gctggagcgg cggtgcggtg gatcgtcttt ccacgcgcct gcaggatgcg
    15541 ggtttcgaaa tgtcgcttag cctgaaagcc aaatggcgac cagaccagga cgctctggag
    15601 ttatgtcgtg aacacggtcg cgaaatcgcc cgtcagtggg cgctcgcgcc gctgccgcag
    15661 agcacggtga atacggtggt taaagaagaa acctctgcca ccacgacggc tgacctcggc
    15721 ccacggatgc agtgcagcgt ctgccagtgg atttacgatc cggcaaaagg cgagccaatg
    15781 caggacgttg cgccaggaac gccgtggagt gaagttccgg ataacttcct ctgcccggaa
    15841 tgctccctcg gcaaagacgt ctttgaagaa ctggcatcgg aggcaaaatg agtaacggca
    15901 ttgtgatcat tggttcgggc ttcgccgccc gccaactggt gaaaaatatt cgcaaacagg
    15961 acgccactat tccattaacc ctgattgccg ccgacagcat ggatgagtac aacaaacctg
    16021 acctcagcca tgttatcagt caggggcaac gtgccgacga ccttacccgc cagacggcgg
    16081 gtgaatttgc cgagcagttt aatctgcgcc tgtttccgca cacctgggtg acggatatcg
    16141 atgccgaagc ccgtgtggtg aaaagccaga ataatcagtg gcaatacgac aagctagtgc
    16201 tggcaaccgg tgccagcgcc tttgtcccgc cagtgcctgg gcgtgagtta atgctgacgt
    16261 taaatagtca gcaagagtat cgcgcctgtg aaacgcaact gcgggatgcc cgacgcgtgt
    16321 tgattgtcgg tggtggcttg attggtagcg aactggcgat ggatttttgt cgtgcaggca
    16381 aagcggtcac gctaatcgac aacgctgcca gtattctggc gtcattaatg ccaccggaag
    16441 taagcagccg cttgcagcat cggttgacgg agatgggcgt tcatctgttg ttgaaatctc
    16501 agttgcaggg gctggaaaaa acagattctg gcattctggc aacgctggac cgccagcgca
    16561 gtatcgaagt ggatgcggta attgccgcca ccggactgcg cccggaaacc gccctggcac
    16621 gacgcgccgg gctgacgatt aatcgcggcg tttgcgtcga tagttatctg caaaccagta
    16681 atgccgatat ttacgcgctg ggcgattgcg cggaaattaa cggtcaggta ttgccgttcc
    16741 tccagccgat tcaacttagc gcgatggtgc tggcaaaaaa tcttctcggc aataacacgc
    16801 cactgaaact accggcgatg ctggtgaaaa tcaaaacgcc ggaattaccg ctgcatctgg
    16861 caggcgaaac ccagcgtcag gatttacgct ggcagattaa taccgaacgc cagggaatgg
    16921 tggcgcgcgg cgttgacgat gctgaccagc ttcgcgcctt tgtggtcagt gaggatcgga
    16981 tgaaagaggc atttggattg ttgaaaacgt tgccgatgta ggtgggctac tgtgcctaaa
    17041 atgtcggatg cgacgctggc gcgtcttatc caacctacgg ggagcgcatg tgttggtcac
    17101 atactgcgtt catgtcgcat ccacctactg tgcccaaaat gtcggatgcg acgctggcgc
    17161 gtcttatccg acctacgggg acgcatgtgt aggccggata aggcgtttac gccgcatccg
    17221 gcaatggtgt ccaaatgcaa cacgttttat ccgttctgga cttcacccgc taaccaacgc
    17281 gccgcagcaa taaccccctg ccccagagac aaaccgccat cacccgccgg taaactctgt
    17341 ggaaagagca atgtgaaatc agcgagataa tgcgccagac gtgcacgcag caaacggtta
    17401 tgaataaccc cgccgctaaa taccagcgta gtgataccac gcatcgtggc ctgctcacgc
    17461 atcaacgcgg caaaaccctg cgccagcgca tcatgaaacg cccacgcgcg ttgattaacc
    17521 ggtgcctgcc agttcagcca ctgctgccag aaagtggcga gatccagttg attgtccacc
    17581 agcggcattg tcaccggatg cgtcactccg tggcacgaag ctgcgagtgc ctccagagca
    17641 caagccgctt caccttcata acttaacgtg gctggcgcac agcccagtgc cgccgccacg
    17701 gcatcgaaca accgcccaca cgatgacgcc agcggcgcgt taattccacg ctcaatggcc
    17761 cgcgccagca cgctccagtt ttgctgttgc acacttgctg tttcagaata attctgccac
    17821 tccggcacaa agcgcaggca ctgcgccagc aggtttcgcc acggctgctt cgccgccaaa
    17881 tcgccacccg gaagcgccac tgcaggcaag ccgcccaggt gctcacattc gcgatagttc
    17941 acccgcagac actcgccgcc ccacaaagcg ccgttctccc ccataccgat accatcgagc
    18001 gtcaaagcaa tgacatcccc gccatccagc ggccactgat gctctgccag acacgccgct
    18061 gcgtgggcat gatgatgcag caccgtttgc gtcggcatat tcatttcgct cgcccactgg
    18121 ctggagacat agcccggatg cacgtcatgc acaacgtatt gcggagtgaa atcgtagatg
    18181 ttttgcatca ggcgtaacgc ttcgcgccac tgcatctgga tgccatcgtc acttaaatcg
    18241 cccagatgct gactcaacac cgcttgttca ccgcgcacca ggcagaaggt gtttttcaga
    18301 tccgcgccga gacacagcac aggcggaaca tttttaaagc ccggaggcaa agccagcgca
    18361 tccggcacat acccccgcga acggcgcagc atttcgccgc tttcgcgcac caccgaatca
    18421 tccatccgct gcacgatgtc gcggttatgt atcaagaatc cgtcggcaat gccctgcaaa
    18481 tccgccagcg cctgttcgtt gctgatagct ggtggtttac cgctcaggtt gccggaggtc
    18541 atcaccagcg ggcattgcag ttcctgtaac agcaaatgct ggagcgggtt cgcaggcaac
    18601 attaccccga cttcgttaag gtcaggggcg atatcatcac aaagctcagg aacgtatttt
    18661 ttatccacca gcacaatcgg cgcggcgggc gtggtaagca actggcgcgc agcgtctggt
    18721 aaaccgtcag ccactggcaa catgactgcc agcggtttcg ccgggcggtg tttgcgctcc
    18781 cgcagtgtcg ccaccgcgtt actgttacgt gcatcgcagg caagatgaaa tccgccaatc
    18841 cctttgatgg cgacaatttt gcccattttt aactgtgcga tagctgcctg taatgccgcc
    18901 tcttgttccg catgttcacc atgacttacc cattcaagat gcgggccaca ctccgggcag
    18961 gccaccggct gggcgtggaa gcgacgatcg agcgggtcgc ggtactcttt atcacaggct
    19021 ggacacagcg gaaactccgc cattacggta aacgggcggt cgtaaggcat ggcgcgaata
    19081 atggtgaaac gcgggccgca gtgggtacag ttgataaacg gataacgata acgccgttcg
    19141 cctggggtat tcatttcggc aaggcaagca gggcaagtag cggcatccgg gacaatttgc
    19201 gtattcatgg cacctcccgc gctctggcgg atagtgaact cggtgggcag ttgtgaccag
    19261 ataaacggct cacgctcgac gctatcaata cgcgccagtg gcggacagtg ctgatgcaat
    19321 tgaacaagaa acgtttccgg gtcttccagc agccggactt ctacgccatc gccgtcatta
    19381 cagacatcgc cgtgaagatt taattgctgt gccagctgcc agacaaacgg acgaaaaccg
    19441 acgccctgca ctttgccacg aatacgcagt tggacaccgc aagatgtgtt ttttgccatt
    19501 gagttattcc cgccatcatg aattgcgtaa cccgtccagc cggacacgac agcgtcgcat
    19561 ccggcagtca caggtcggcg ataccgcccg cttcgtattc tacgaatatt tccgggaatt
    19621 cctttgatgc cagaacagtt ctgtaagatt tttagaacat cagcgccgta cggcggcgtt
    19681 tttctgcgct cagttgttca agtttattac gatcgacaca aattagcgca tgggtcgggc
    19741 aagccgccat acacgccggg ccgtcttcac gatggttgca caggtcgcat ttattggctt
    19801 cagctttgtc agcccgtaca ttcagcccca cgccgctgtt gcgaatcacc ggacgtacca
    19861 ccacttccat cgcgccatac gggcaagcca ccacgcaggt tttgcaacca atacaacgtt
    19921 cctgcatcac atgaacaaac cctttatcac ggctgatagc accattcggg cagacgttag
    19981 cgcacggtgc atcttcacac tgacggcaaa ctgtcgccgt ggaaatgttc acacctttaa
    20041 tgacatggat acgcggtaaa aatgtttccg gggtcagcga tgcacagtcc tgattttcct
    20101 gatgagaaac cacgcacgcc acttcacagg tacggcaacc aatacattta ctcgcgtcag
    20161 caatgatgaa acggttcatc aaattctcca gcaatgacag ttaatgcgcc gatacattca
    20221 caaatcatgc cagtttttaa tttactgtta tttaaggaaa ttaatttctg taatgcagga
    20281 aaaacgatgt catcgacact agtgacgatg acatgtgatg acaatgttta tcgcgaaagg
    20341 gcaatgagtg agtcgcggca gatcaatttt ccgctgaagg ttttcggcgg tgagaaatcc
    20401 ccgccatcga gcataaaaat cagccgtcca ataatttcct gaatcatctc agtcaccgga
    20461 atttttacgc tggagagcgc cggaacgata tagggggcaa ttgcgatatc atcgaatccg
    20521 ataactgaca cctgctctgg caccgctacg ccgcgctcgt gcaacgcttt catcgcacct
    20581 atcgccatat cgtcgttact ggcaactaac gcgctaaatt tagccccacg ttcgagcaac
    20641 atttctaccc cttcggcccc gctggcaggc gtccatctac cgttagcgat aagtttttca
    20701 ttgagcgcaa taccgtgctg cgccagcgcg tctttatatc cggcaagacg ttcaacgctg
    20761 gtgggagaat ccatcgaacc ggtaaggaaa gcaatctcct gatgtccggc gtttatcaac
    20821 tctgccacgg cgttaaaact ggtctgttta tgatcgcacc agacgctatg gctgctgttt
    20881 ttgcgcaggc ggcgattaag caccattatc ggctgactgt gcgcgtcaat gatgtcatcg
    20941 atctcatcca cgcttaaaaa acgcgggtaa atcataatgg cgtcgcagcg caaatccagc
    21001 agatactgaa tcgcctggcg ctcttcttcg gcgctgtgtt taccatctgc caatagcaac
    21061 tgccgccctt tctcttccgc cattcgcgcg gcatgaaaca gtaattcact aaaataaatg
    21121 ccgtggtaaa gcgtgttggt cactaccagc cccagcgtct gagtactctt cgccgacaaa
    21181 ttgcgcgcca gcaagtttgg gcggtaaccg ctctcttcta ccgcctgaaa cacgcgatct
    21241 ttagtctcct ggctgacgta gccattacct gaaagcacgc gggaaacggt cgcttttgaa
    21301 accccggcgc gcttcgccac ttccagcatc gtcgtcatca ttttcatccc tttacacgca
    21361 atcaacgcag tgtactgcac cgtttgccga ttgtccttgc gcaatcggcg ggaaaaatat
    21421 tcaggtgacc ggtttcacaa atataaaaaa tgaacaatta actctcttgc ttatttagtg
    21481 acaactattc atgattttgt gaaaccggtt tcctaattcc gtttcagcat cggcattttt
    21541 ccgttacgtc gactgataac aactacatct accctactga taacaggata aaatccgatg
    21601 gccaaaaatt atgcggcgct ggcacgctcg gtgatagcgg cactgggcgg cgttgataac
    21661 atctcggcgg tcacgcactg tatgacgcgt ttgcgctttg ttatcaaaga tgaccagctt
    21721 atcgacagcc cgacgttaaa aaccatcccc ggcgtgctcg gcgtggtacg tagtgacaac
    21781 cagtgtcagg tgattatcgg caataccgtt tcacaagcct ttcaggaagt cgtcagccta
    21841 ctgccgggag atatgcagcc cgcacagccc gtgggtaaac ccaaactcac gctgcgtcgc
    21901 attggtgcag ggatcctcga tgcgctgatc ggcaccatgt caccgctgat cccggcgatt
    21961 atcggcggat cgatggtcaa actgctggcg atgatcctcg agatgagcgg cgtgctgaca
    22021 aaaggatcgc cgaccttaac cattctgaat gtgattggtg acggtgcttt cttcttcctg
    22081 ccgctgatgg tcgcggcatc tgccgccatc aaatttaaaa ccaatatgtc gctggcgatt
    22141 gccatcgcgg gtgtgctggt acatccgagt tttattgaac tgatggcgaa agcggcccag
    22201 ggtgaacatg tcgaatttgc cctgattccg gtcaccgcgg tgaaatacac ctacacagtg
    22261 atcccggcgc tggtgatgac ctggtgcctg tcatatatcg aacgctgggt ggatcgcatt
    22321 acaccggcgg tgaccaaaaa cttcctcaag ccaatgctga ttgtgttgat tgccgcaccg
    22381 ctggcaatcc tgctgattgg cccgattggt atctggatcg gtagcgccat ttcggcgctg
    22441 gtttacacca ttcatggtta tctgggctgg ctttcagtcg ccattatggg cgcgctgtgg
    22501 cctctgctgg taatgaccgg gatgcaccgc gtctttacgc caaccatcat tcagaccatt
    22561 gccgaaaccg gcaaagaagg gatggtcatg ccgtcagaga tcggcgctaa cctgtcgctg
    22621 ggcggttcat cactggcggt ggcgtggaaa acgaaaaacc cggaactgcg ccagacggcg
    22681 ctggctgcgg cggcatcagc cattatggcg gggatttccg aaccggcgtt atacggcgtg
    22741 gcgatccgcc tgaaacgtcc gcttatcgcc agtcttatca gcggctttat ttgcggcgcg
    22801 gttgccggta tggcggggct tgccagccac tcaatggcag cgccggggct atttaccagc
    22861 gtgcagttct tcgatccggc gaatccaatg agcatcgtct gggtgttcgc ggtcatggcg
    22921 ctggcggtca tgctgtcgtt tatcctcaca ctgttgctcg gctttgagga tattcctgtt
    22981 gaggaagcga ctgccgaggc gcgaaagcat cagagcgtac aaccgaccgt cgccaaagaa
    23041 gtaagtctta attgaggatg aaaatgtcag tatttccaga aggtttttta tggggcggcg
    23101 cgcttgccgc caaccagtct gaaggtgcgt tccgtgaagg tggcaaaggg ctgaccactg
    23161 tcgatatgat cccacacggc gagcatcgaa tggcggtgaa actggggctg gaaaaacgtt
    23221 ttcagttgcg cgatgacgag ttttatccca gccatgaggc gacggatttt tatcatcgtt
    23281 ataaagaaga tatcgccctg atggcagaga tgggattcaa ggttttccgt acctcaatta
    23341 cctggagccg tctctttccg cagggcgatg aaatcacgcc caatcagcag ggcattgctt
    23401 tttatcgttc tgtctttgaa gagtgtaaaa agtacggtat cgaaccgctg gtcacgttgt
    23461 gccacttcga tgtgccgatg catctggtca ccgaatatgg ctcctggcgt aaccgcaagc
    23521 tggtggagtt tttcagccgc tacgcccgga cctgctttga agcatttgat ggtctggtga
    23581 aatactggct taccttcaat gaaatcaaca ttatgttgca tagcccgttc tccggcgcgg
    23641 gtctggtgtt tgaagaaggt gaaaatcagg atcaggtgaa atatcaggcc gcgcatcacc
    23701 agctggttgc cagtgcgcta gccaccaaaa tcgcccatga ggttaacccg caaaatcagg
    23761 tgggctgtat gctggcgggc ggtaacttct acccttacag ttgcaagccg gaagatgtct
    23821 gggcggcgct ggagaaagac cgggaaaacc tgttttttat cgatgtgcag gcgcggggcg
    23881 cgtatccggc ttactctgcc cgcgtattcc gcgaaaaagg ggtaaccatc gacaaagcac
    23941 cgggcgatga tgaaatcctg aaaaacaccg tcgattttgt ctctttcagc tattacgcct
    24001 cgcgctgcgc ctcggcggag atgaacgcca acaatagcag tgcggcgaac gtagtgaaat
    24061 cgctgcgtaa cccgtatctg caagtgagcg actggggctg gggtattgat ccactcggtc
    24121 tgcgtatcac catgaacatg atgtacgacc gttatcagaa gccgctgttt ctggtggaaa
    24181 acggcctcgg cgcaaaagat gaacttgctg ccaatggcga gattaacgat gactatcgca
    24241 tcagctattt acgcgaacat atccgcgcaa tgggcgaagc gattgcagat ggcattccgc
    24301 tgatgggcta caccacatgg ggctgtattg atttagtttc cgcctctacg ggtgaaatga
    24361 gcaaacgcta cggttttgtc tacgtagacc gtgacgacgc aggcaacggc acgctgacgc
    24421 gcacacgtaa gaaatcgttc tggtggtata aaaaagtgat tgccagtaat ggtgaagatt
    24481 tagagtaagc aacagtgccg gatgcggcgt gaacgcctta tccggcctac atgtcgcacg
    24541 aaccaggaag gcccgataag cgcagcgcat cgggcaatct ggggtttggc atcagtcaaa
    24601 atcaaagaaa ccattccggc ttaacgccaa atcgggctga aagtgcctta atgtgggata
    24661 tcgtcaggga tcgttgtcca gacaaaatct gactgaccag ggatttagag ccgatttcgt
    24721 tcttcaaatc cgcataggtc agattatgtt gatcaatcag cgtgcgaagt aacgccacgc
    24781 caacaggcat tgccgcaacg gctttgtcga actctgcaaa cttttcgttg ttgttttcat
    24841 actctgcaat gcggctcgcc agaaagtcga taagtggatt ggtgtcatct tcttcaatca
    24901 aatattcgac cagttccagt gcttcacgat aatcatcttc tgagtcactg cctcccagaa
    24961 aggggactgc attgaccagt tctcttgttg ctttaacagc gcgtgcagca ttagcagtca
    25021 ttctttgttc tctctgtagt aacgtgtcag tttgtcatac tctgcgtgat tcgttatgtg
    25081 cttcacaaag aaccgcttat taacaaagtt tatgtaggca ataaccctaa gattgttccc
    25141 gccgacatcc agaacgtacc atttgttgcg atatctaaaa ttgtcgaggt tgggaaacgc
    25201 cgttcgcatt tcttctggtg tagaaaaatc cgtttcctta ataacccgat acaatgcctg
    25261 atgagccaga gcgtcgttag gatatttacg cgcagactct tcaaatggcg ctttagaaat
    25321 gatgtgcatt tttaagtttt aacttcctta tagtaagtaa acagccacat ctgcgatgtt
    25381 ctatccttat gtgttcggaa actatagctc atcaaatcca agttcacaat atgtaaacac
    25441 ttgtgcgcga tataattacc cccaaaatcc tcatagccac tctgcaaaaa gtcagttctg
    25501 gttcacccct caagcacatc cccctttatc aagcaacgct gcgtctgcgc cagatcttcc
    25561 gtttgcgggt cgcgcccggc gatgggtttg aggacgagtt tacggttggg ggtataagta
    25621 aaacgcgtga gcgcgggata gcttaacttg ccaaaccagc cgtgaacacg ctgccatttg
    25681 gcgtctttta cctgatacag actggttatc cagtaaccag aattaaactg catatccagc
    25741 aattgcgtgt gaccattgcc ttgcaaatcc agcaaatcat ctacgccagt tttgcttgcg
    25801 gtgtagaatc ccctgggttc aaagacacag ggacggcctt cgcgcgtgaa cgtcataaga
    25861 attaaatgtg cataaggagc cagaccatta ccggtacttg gtaaccagat aaccagatcc
    25921 tgaatgccgt tacgatcaag atcggcgcga tagagttgcc ctttgcccgc tatgtttgcc
    25981 agcataccca caggcacagt ccaggattta ccggcgcggt ctttagcggt taattgccag
    26041 taatcttctt catttggcat cttctcaagc gtcacgccag caacgcccac tttcctaaaa
    26101 tgccaggttt gctcggcatc ataaggcatg gtcaggagag gtttcagcgc ccgcttttgc
    26161 gcgtattcgc tgtcactcat tggcagatat tcggtaaagc tggtggcctg tgcaggcagc
    26221 actaagctca ataaaaaagc gcacatccct gttctgatgt tcaattccct gaattccctg
    26281 atggagaatt attcttcttc caccgctaac tgcgcaaatc caccatttcc ttcccagcct
    26341 tccagtcgtt gataaacggt ttctaccgca tctttaatcg gctgggtcat cgggtagtaa
    26401 aagccgacga tatccggctg aatgccgagg aaaatcactt caccaatgtc ttcttttaac
    26461 tggtcgataa ggtaattcaa cggcatgtta tgggtagtca tcataaacat ctcggcgata
    26521 tcatccgggt cgatgatgcg gatttcgccg gggtttagcc ccatatccgt ggcgtcgaca
    26581 atcagcagtc gtgtcgggcg cagttcacgg atggcgacga tgtcgttttc cggtgcacta
    26641 ccgccgtcaa tcaccaccca gttacctttc ggcgcggcgg cgcacttttc cgccagcagc
    26701 gggccagcgc catcatcgcc catcatgcta ttgccaacac agagtaaaac gtcagtcacg
    26761 cagtctcctc accatcaaat agatggcgtt ttcctgatga atatcatgca gcatacttaa
    26821 cagcattgta ctccattcct tctgctgcga agtttgcgct gcccgggctt tatcgaatgc
    26881 gttggccagc atcggcacat ggttaatgtc gatgacgatc tcgccatatt tcggtacgcc
    26941 ttccattttg cggcgggctt cgctgcccgc ctccagagtg gcaatccatg ccagatattc
    27001 atcccatggg caggtgagcg ccgcttccag gcaatcgata accccaaggt ggtgaccaat
    27061 cgccaggctg taatagacca cctgctgcgc ctcggcgggc gtggcatcgt tctcatcaat
    27121 aaatttacgg ctcagttgac tgaacaccac cttttcactc atcggatacg cgcctcttca
    27181 acaacatgat tcagatggct gacaatctcg ttcagacgcg gatcgttttc cgcttccagc
    27241 cagcgtgcaa cctgttcttc gccctgccct aactgtgtaa ggtaatcatc ggcaatctga
    27301 cgaccgtaac gataacccgc caggcgacgt gcttcgcgat ccactttcac gcgcagcggc
    27361 tgcaccatat cgccatgcag aatctcagcc ggttgttcat cctgctcacc cgggccacgg
    27421 gcgtgaattt tctgctccag caggccgagt gccattgcaa agccgtacag cgtggcggca
    27481 ggcgttggcg ggcaacccgg aatgtagaca tctaccggga cgattttatc cgtaccgccc
    27541 cacacgcagt agagatcgtg gaaaatacca ccgctgttac cgcaggcacc gtaggagata
    27601 caaattttcg ggtccggcgc ggactgccac gcacgcagcg caggggatcg cattgcacgg
    27661 gtgaccgcgc cggtaaacag taaaatatcc gcatgacgcg gtgaaggaac gactttaatg
    27721 ccgaagcgtt ctgcatcaaa cagcggcgat aaagtggcga aaatttcgat ttcgcagccg
    27781 ttgcagccgc cgcagtccac gcggtaaaca taggcagaac gtttgatttt tttcagtaac
    27841 gacgccttca tgctggcgat ggattcatcc accgtcatcg ggaccggaat gccgttggcg
    27901 tcacgggggc ctaataaatt gctcatcaga tggcctcttt catatggcga gtcagttcaa
    27961 tacggtcgga cggcaccagg catttctggc gcttacattc cgggcaagtc tcaaagcttt
    28021 cgcggtggtt ttccgcgcgg ctgtcaccat tgtgcttaag cagcgcaatg gcgtagtcga
    28081 tctctttctg gacggcgaaa ggacgattgc agacgcgaca gctgcacagc acaaaacggg
    28141 actgttgcag gaaatcttct ttcttccaca ccgccagttc gtactcttgc gacagtttga
    28201 tcgctgccgt cgggcagact tcttcgcagc gtccacagaa gatgcagcgc ccgagattaa
    28261 actcccacgc cagctcaccg gtggcgaggt cagtttcaac cgttaaggcg tttgacgggc
    28321 aggcattgac gcaggccgca cagccgatgc actgctgcgg gttctgctcc ggcttaccac
    28381 ggaagttttt atcaaccgca atcggctcca gcggataaga cgaggtcgcc gtgccggttt
    28441 tgatgacttt tttgataaag gtaaacatgg cgattcctta tttcagcggc gagtttttac
    28501 gctcaatgct gtaacgctcg agttctttgt acggcaccac tttgctcttt ttcttacgca
    28561 catcgaccac ggtcatgcgg tcggtacagg agtagcaagg gtccaggcta ccgataatca
    28621 gcggcgcatc ggaaacggtg ttaccgcgca gcatgtagcg cagggtcggc cagttcgcgt
    28681 aggtcgcggc acggcagcgc cagcggtaca gcttctggtt gtcgccggtc atgctccagt
    28741 ggatatcgtc gccgcgcggc gcttcggcaa agcccagcgc gaaacggtgc ggaatgtagg
    28801 taaagccttc caccatcagc ggaccgcccg gcaggttatc cagaccgtag tcgatcatgt
    28861 tcagcgcggt ataaacttcg ttgatacgca ctttcagacg ggaaataacg tcgcagccct
    28921 gctcgctgtg gacttccatt ggcagcaggc cataaccgac aaacgggtga tcggcgcgag
    28981 tatcgcgggc gtgaccgctg gcgcgaacca tcgggccaac gttactgaaa tcgcgggcga
    29041 tttccgggtc cagacgacca atgccgacgg tgcgctgttc catgttcggc gtactcagca
    29101 gcacatccac cagctcctgc acttcacgac gcatctgttg cgccagctgg cgggtctgga
    29161 tcatgtcatc tttcaacaga tcgcgacgaa tcccgccgat caggttcagg ccgtaggttt
    29221 tacgcgcacc ggtgaggatc tctgccattt tcatggaggt ttcacgcacg cggaagaact
    29281 gcataaagcc ggagtcaaag ccggtaaagt ggcaggcgag accgaggttg agcagatgcg
    29341 agtgcaggcg ttccacctcc agcagaatgg cgcggatcat ctgcgcacgt tctggcacct
    29401 gaatacccat cgcgttttct accgacgtgg tgtaggcggt gctgtgggca aagccgcaga
    29461 tcccgcacac acggtcagag aggaaggtca cttcgttgta acccatacgg gtttccgcca
    29521 gtttttccat gccgcgatga acgtagaaca gacggtagtc ggcgtcgata atgttttcgc
    29581 catcgacgaa cagacggaag tggcccggtt cgtcagaagt gacgtgcagc ggaccaatcg
    29641 gcacgacgtt gtttttcttg tcgcccagtt cgttgatgaa ctcgtaggtt tcagcatcgg
    29701 tagtcggtgc cggacgctga cgataatcca tgctgtcttt acgcagcgga taaagttcat
    29761 ccggccagtc atccggcagc accagacgac gttcatccgg cagaccaacc ggaatcaaac
    29821 cgtacatatc gcgcacttcg cgctcgcccc acaccgccgc cggaacgcgc ggcgtcacgg
    29881 acggatactc cggtttgttg gcgtcaactt cgacgcgaac cgttacccaa cacttggtgc
    29941 ccttctccat cgacagcacg taataaacgg cgtaatgacc attcagtttg cgttcgtcgt
    30001 taccaaacag caccgacagc cagccaccct gcttgtagta gagaaactcc accacttccg
    30061 gcaggtagtt caccttcacg gtcacggtca gctgatcttt ggtctgccag gcgtggtcca
    30121 gcacgacgcc cggaaatgcc tcattcagcg cggcgagata atgttgacct aatttttctt
    30181 cagacatgct caaactctct ttaatcacgc cgccagcaag gagacgaacg ctaaaaatgc
    30241 aaagccaaac ccagcccagg taatgcgcgg agtaatatca agacgcagac gggccatgct
    30301 gttttcgaac agcgcgataa ccaggacgcc gacgaccagt ttgacgatgg caatcaccag
    30361 cgccagcagc agcccaccgg cggtgaaggt ttccatctgt ccccacggga taaatacgcc
    30421 gacgaacatc tgcagcacca ccagctgttt caggctgata ccccatttca tgacgccaaa
    30481 gccgctgccg ctgtattcag agagcgggcc ttcctgcagc tcctgctcgg cttccgccag
    30541 gtcgaacggc agtttgccca tttcgataaa ggtggcgaac gcacaggcac aaagcgccag
    30601 taccagcggg atgctctggc tcagcggcca gtgataaacg gtgtcggtga tgttgctgat
    30661 gttggtggaa ccggcaacct gtgcggcaac ccacagacca agcagcagca tcggttcgac
    30721 cagcacgcca agcatcgctt cacggctcgc gccgatagcg gtaaacgggc taccggtatc
    30781 cagaccagaa atggcaaaga agaaacgcgc gatggcaaag agatacagta aggtgattaa
    30841 atcacccagt tgcggcagcg gagaaccgac ggtcaccacc ggcagcgcag tagcgatagt
    30901 cagcatgacg cccaccatca catacggcgt caggcggaac acccagccgg aggcatccgg
    30961 gccaacgctc tggcggccca gcagtttgat aatgtcacga tactcctgca acacacccgg
    31021 cccacgacgg ttatgtaagc gggctcgcgc cacgcgggta ataccggaga gcagcggcgc
    31081 aacggcaaat agcaccagcg cctgaattaa cggatataaa acactcattc tcaggctcct
    31141 cgtgaaacaa taatcaccac cagtaccgcc agttcaacca gcgccatccg gcggaacagc
    31201 aacgcactcc cctcgcactg ccagcccggc accagagaca ccggattcag ccatttgcgt
    31261 agtttcagca ccggcgcaaa cgcctgtttc accggcatgg caaaaccgtg agcggtaatc
    31321 accattgatt tttcgtggtc gtagccgcac acccaggccg cgccgcggga acgcgatggc
    31381 aaacgatcgc ctttgcaaat cgccataatg atgaatggca gcagcggaca ggcaatcagc
    31441 agcaatgtga tcattggttg agaaacggtg gtgttagcag gctccagcgg cagaggtaca
    31501 gcagcagaca gcatcggcag cagccacggc gcagcaacac cgccaattac gcagcaaatc
    31561 gccagtgcca ctacgcttac cctcatcagg agcggcgcac aggtggcgtt ttcggcttct
    31621 ttggtacgcg gcgcgccgag gaacgtgacg ccatagactt tcgccataca catcaccgcc
    31681 agcgcaccgg taattgccag acccacagcg agcagcggcc ccagcagacg ggcaacaaac
    31741 gcgccgctat tgctcagttt gaagaaggat tgatagataa cccattcccc ggcaaacccg
    31801 ttcagcggcg gcagcgcagc cattgccatt aacccgacta acatggcgat ggagataacc
    31861 ggcattttct tgccaatacc accgagtttt tcgatatcgc gatgaccggt acggaaccag
    31921 acgctccccg ccccgaggaa cagtacgctt ttgaacaggc tatggttaag cagatggtac
    31981 agaccaccga ccaggccaag agcaatcagc gccggttgtt cgagcgcgat acccgttacg
    32041 ccagcgccca gccccagcag gataatgccg atattttcca gggtgtggta agccagcagg
    32101 cgctggatgt tgtgctccat cagcgcatac agaccaccga caaacgcggt gatcatgccg
    32161 agcaccagca gcgcgatccc ccaccacagc ggtgcattac cgcccagcag tgacagggtt
    32221 aaaatgccca gcaggccaat tttcatgact accgtagaaa acaacgcggc agctggcgca
    32281 gaggcgttag catgtgcctg cggcacccag ccgtgcagcg gaataatccc ggcgagcagg
    32341 ccaaagccaa tcacaccgag cagccagata tcggaaccga gcggcagctg ttgcatacgc
    32401 atatccagca ggcgcagatc cagcgtgcca taacgctgcc acagcagcca gcaagcaatc
    32461 gccagcagca gagtgccaag acgccccagc gcaaaccaca gtttgccctc tttgctgttg
    32521 ctagtgagga acaccgcgca cagggccatg atttcggcca ttaccacgaa catgccgagg
    32581 ttgctggcaa tgacggcgca gacggcggca gccatcaaca tattgatctg caagccgttg
    32641 cacttcacct gcgcgtggcg atgccagtca atgttataga ggctgacaaa cagaccgcaa
    32701 aggccgaggg taatcagcca aatcgcgtta agcggggaga tttgcacatc gtagcttacc
    32761 agcgacagcg caccgctcac gccaaccgcg ccagtcagca cagtgaagcc cgcggctgcc
    32821 gtatacaggc taccaaccgc gccgccaatt ccggctatcc agccacttag cgtcttttga
    32881 aaagaaaaga gaaatgccag aacagcggcg gcgacaaacc acgccacgcc gctattgatc
    32941 agggaaattg cgctcattta gcctctccac tttgagcctg ctgaaacaag gtgagatcgc
    33001 caaagtccgt gttaaaggtc agctcacgct tacgtttgct gacgcgggcg atatcggtgt
    33061 tatccaccag atgcagggct ttagtcgggc acatccgcac gcaggccgga ccttgttcat
    33121 caaagctaca aaggtcacat ttgacggcga tcgcgcgaat acctggcacc cagtcaagca
    33181 atgtgctgac acgcgccgga gcaggcggtg ccggtggcgc tttcggggta ttggcgtttg
    33241 ccggaatatc cagcggacgg ctgccggaaa attcaattgc gccaaacggg caggcgatgc
    33301 cgcacagctt gcagcttacg cacaggcttt cattcaactg cacggcccca tcaacgcggg
    33361 tgatggcgtt aaccgggcag accaccgcgc agggtgcatc ttcacagtgg tgacagagct
    33421 gcggcgcaga ttctttttca ttcagcatca ctctcaggcg cggcattgat tgcaggccgt
    33481 gctggcgatg cgtctctgaa caggcggcct cacaagtgtg gcagccgata cagagcgtgg
    33541 agtcagcaat tacaaaacga ttcaccaggc attcctcagg tgattgtcat ttttgacgaa
    33601 aacatgccga tgaaatgtca ttttcgacac tcatcgacac gcccatcccc aaacaggcgt
    33661 aacgcctgca aaacgggcaa agcctcagct catgctgccg ggctttgtcc ctttaccagt
    33721 tggcttaaat tcaccggcac attgttttca atagcggtgt ataagctgtc gtaaacgcca
    33781 gcgatttttt cgagatagtg ttccagaacc tgttcgcgat cgcggatgtt gaccttgcca
    33841 tcaatcatgc cgtcaatgct cagttgcgcc tgcgcaatcc gctgtttgtc ttcgccatct
    33901 tttgtttcga cgtaatactc gatggtgtgg ctgttaaagc catccgccag ggtgacgtaa
    33961 atgcgaaaat gttcaaaaag gacgaaacag agttctttgt ccggcgcgca gctcatggcg
    34021 tgatgcaggc gcgctttcga taacgtgatc ccctgaacca gcgaattgca gtagatgtgc
    34081 cactggtcct gtaggcgacg atgccgctgt gcgatgtaat cggctttctc gcttatttcc
    34141 caaatagtca ttgtcaggtt acccgtttaa cagagatgcc agctttaagc attttctgtg
    34201 ccaactttta atttattgtt attaaagaga tttttaagct aaagatgaat ttcgtcgccg
    34261 tgtcgacgtg tcatttcgac atcatcgaca ttattcaccg cagggataat caacactggc
    34321 acaattattg cttgtagctg gcaatagtta atgggaggcg atatgcacga aataaccctc
    34381 tgccaacggg cactggaatt gatcgaacag caggccgcaa aacacggcgc aaaacgcgta
    34441 actggggtct ggctcaaaat tggcgcattt tcttgtgtcg aaaccagctc tcttgccttt
    34501 tgttttgatc tggtttgccg cggcagcgtg gcggaaggtt gtaaactgca cctcgaagaa
    34561 caagaggccg aatgctggtg tgaaacatgc caacagtatg tgacgctact gacccagcgc
    34621 gtccgccgct gtccacagtg tcatggtgac atgctgcaga ttgtggcaga cgacggttta
    34681 cagattcggc ggatagaaat agaccaggag tgagcgatgt gtacaacatg cggttgcggt
    34741 gaaggcaacc tgtatatcga gggtgatgaa cataaccctc attccgcgtt tcgtagcgcg
    34801 ccatttgccc cggcggcacg cccgaagatg aaaatcaccg gcattaaagc gcctgaattt
    34861 acccccagcc agactgaaga aggcgacctg cattacggtc atggcgaagc gggcactcac
    34921 gcaccgggca tgagccagcg tcggatgctg gaagtcgaaa ttgacgtgct ggacaaaaat
    34981 aaccgtctgg ctgaacgcaa ccgcgcgcgc tttgctgccc gcaagcaact ggtgctcaac
    35041 ctggtttcca gcccaggttc cggtaaaacc accctgctga cggaaacctt aatgcgcctg
    35101 aaagacagcg ttccgtgcgc ggttattgaa ggtgaccagc aaaccgtgaa cgatgccgca
    35161 cgcattcgcg ctaccggcac accagcgatt caggtgaaca ccggtaaagg ctgccatctt
    35221 gacgcacaga tgattgccga cgccgcaccg cgtctgccac tggacgataa cggtattctg
    35281 tttatcgaaa acgttggcaa cctcgtatgc ccggccagct tcgatctcgg tgaaaaacac
    35341 aaagtggcgg tgctttccgt taccgaaggt gaagacaaac cgctgaaata tccgcatatg
    35401 tttgccgccg cctcgctgat gctgctcaac aaagttgacc tgttgccgta tctcaacttt
    35461 gacgttgaga agtgcctcgc ctgcgcccgc gaagtcaatc cagaaattga aatcatcctt
    35521 atttccgcca ccagcggtga agggatggac cagtggctga actggctgga gacacagcga
    35581 tgtgcatagg cgttcccggc cagatccgca ccattgacgg caaccaggca aaagtcgacg
    35641 tctgcggcat tcagcgcgat gtcgatttaa cgttagtcgg cagctgcgat gaaaacggtc
    35701 agccgcgcgt gggccagtgg gtactggtac acgttggctt tgccatgagc gtaattaatg
    35761 aagccgaagc acgcgacact ctcgacgcct tacaaaacat gtttgacgtt gagccggatg
    35821 tcggcgcgct gttgtatggc gaggaaaaat aatgcgtttt gttgatgaat atcgcgcgcc
    35881 ggaacaggtg atgcagttaa ttgagcatct gcgcgaacgt gcttcacatc tctcttacac
    35941 cgccgaacgc cctctgcgga ttatggaagt gtgcggtggt catacccacg ctatttttaa
    36001 attcggcctc gaccagttac tgcctgaaaa cgttgagttt atccacggtc cgggttgccc
    36061 ggtgtgcgta ctgccgatgg gtagaatcga cacctgcgtg gagattgcca gccatccgga
    36121 agtcatcttc tgtacctttg gcgacgccat gcgcgtacca gggaaacagg gatcgctgtt
    36181 gcaggcaaaa gcacgcggtg ccgatgtgcg catcgtttac tcgccgatgg atgcgttgaa
    36241 actggcgcag gagaatccaa cccgcaaagt ggtgttcttc ggcttaggtt ttgaaaccac
    36301 catgccgacc accgccatca ctctgcaaca ggcgaaagcg cgtgatgtgc agaattttta
    36361 cttcttctgc cagcacatta cgcttatccc gacgctgcgc agtttgctgg aacagccgga
    36421 taacggtatc gacgcgttcc tcgcgccggg ccacgttagt atggttatcg gcactgatgc
    36481 ctataatttt atcgccagcg attttcatcg tccgctggtg gtcgctggtt tcgaacccct
    36541 tgatctactg caaggcgtgg tcatgctggt ggagcagaaa atagcggccc acagcaaggt
    36601 agagaatcag tatcgtcggg tggtaccgga tgccggtaac ctgctggcgc aacaggcgat
    36661 tgccgatgtg ttctgtgtca acggcgacag cgaatggcgc ggcttaggcg tgattgaatc
    36721 ttctggcgtg cacctgacgc cggattatca acgattcgat gccgaagcac atttccgccc
    36781 ggcaccgcag caggtctgcg atgacccgcg cgcgcgttgt ggtgaggtat taacgggcaa
    36841 atgtaagccg catcaatgcc cgctgtttgg taacacctgt aatcctcaaa ccgcatttgg
    36901 tgcactgatg gtctcctccg aaggagcgtg cgccgcgtgg tatcagtatc gtcagcagga
    36961 gagtgaagcg tgaataatat ccaactcgcc cacggcagcg gcggccaggc gatgcagcaa
    37021 ttaatcaaca gcctgtttat ggaagccttt gccaacccgt ggctggcaga gcaggaagat
    37081 caggcacgtc ttgatctggc gcagctggta gcagaaggcg atcgtctggc gttctccacc
    37141 gacagttacg tcattgaccc gctgttcttt cctggcggta atatcggcaa gctggcgatt
    37201 tgcggcactg cgaatgacgt tgcggtcagt ggcgctattc cgcgctatct ctcctgtggc
    37261 tttatcctcg aagaaggatt gccgatggag acactgaaag ccgtagtgac cagcatggca
    37321 gaaaccgccc gcacggcagg cattgccatc gttactggcg atactaaagt ggtgcagcgc
    37381 ggcgcggcag ataaactgtt tatcaacacc gctggcatgg gcgcaattcc ggcgaatatt
    37441 cactggggcg cacagacgct aaccgcaggc gatgtattac tggttagcgg cacgctcggc
    37501 gaccacgggg cgactatcct taacctgcgt gagcagctgg ggctggatgg cgaactggtc
    37561 agcgactgcg cggtactgac gccgcttatt cagacgctgc gtgacattcc cggcgtgaaa
    37621 gcgctgcgtg atgccacccg tggtggtgtg aacgcggtag ttcatgagtt cgcggcagcc
    37681 tgcggttgtg gtattgaaat ttcagaagcg gcactgccgg ttaaacctgc cgtgcgcggc
    37741 gtttgcgaac tgctgggact ggacgccctt aactttgcca acgaaggcaa actggtgatc
    37801 gccgttgaac gcaacgcggc agagcaagtg ctggcagcgt tacattccca tccactggga
    37861 aaagacgcgg cgctgattgg tgaagtggtg gaacgtaaag gtgttcgtct tgccggtctg
    37921 tatggcgtga aacgaaccct cgatttacca cacgccgaac cgcttccgcg tatatgctaa
    37981 taaaattcta aatctcctat agttagtcaa tgaccttttg caccgctttg cggtgctttc
    38041 ctggaagaac aaaatgtcat atacaccgat gagtgatctc ggacagcaag ggttgttcga
    38101 catcactcgg acactattgc agcagcccga tctggcctcg ctgtgtgagg ctctttcgca
    38161 actggtaaag cgttctgcgc tcgccgacaa cgcggctatt gtgttgtggc aagcgcagac
    38221 tcaacgtgcg tcttattacg catcgcgtga aaaagacacc cccattaaat atgaagacga
    38281 aactgttctg gcacacggtc cggtacgccg cattttgtcg cgccctgata cgctgcattg
    38341 cagttacgaa gaattttgtg aaacctggcc gcagctggtc gcaggtgggc tataccccaa
    38401 atttggtcac tattgcctga tgccactggc ggcggaaggg catatttttg gtggctgtga
    38461 atttattcgt tatgacgatc gtccctggag cgaaaaagag ttcaatcgtc gcaaacattt
    38521 acgcagatcg tttctgtcgt caccgaacaa atccagagcc gcgtcgtcaa caatgtcgac
    38581 tatgagttgt tatgccggga gcgcgataac ttccgcatcc tggttgccat taccaacgcg
    38641 gtgctttccc gcctggatat ggacgaactg gtcagcgaag tcgccaaaga aatccattac
    38701 tatttcgaca ttgacgatat cagcattgtc ttacgcagcc accgtaaaaa caaactcaac
    38761 atctactcca ctcactatct tgataaacaa catcccgccc acgaacagag cgaagtcgat
    38821 gaagccggaa ccctcaccga acgcgtgttc aaaagtaaag agatgctgct gatcaatctc
    38881 cacgagcggg acgatttagc cccctatgaa cgcatgttgt tcgacacctg gggcaaccag
    38941 attcaaacct tgtgcctgtt accgctgatg tctggcgaca ccatgctggg cgtgctgaaa
    39001 ctggcgcaat gcgaagagaa agtgtttacc actaccaatc tgaatttgct gcgccagatt
    39061 gccgaacgtg tggcaatcgc tgtcgataac gccctcgcct atcaggaaat ccatcgtctg
    39121 aaagaacggc tggttgatga aaacctcgcc ctgaccgagc agctcaacaa tgttgatagt
    39181 gaatttggcg agattattgg ccgcagcgaa gccatgtaca gcgtgcttaa acaagttgaa
    39241 atggtggcgc aaagtgacag taccgtgctg atcctcggtg aaactggcac gggtaaagag
    39301 ctgattgccc gcgcgatcca taatctcagt gggcgtaata atcgccgcat ggtcaaaatg
    39361 aactgcgcgg cgatgcctgc cggattgctg gaaagcgatc tgtttggtca tgagcgtggg
    39421 gcttttaccg gtgccagcgc ccagcgcatt ggtcgttttg aactggcgga taaaagctct
    39481 ctgttcctcg acgaagtggg cgatatgcca ctggagttac agccgaagtt gctgcgtgta
    39541 ttgcaggaac aggagtttga acgtctcggc agcaacaaaa tcattcagac ggacgtacgt
    39601 ttaattgccg cgactaaccg cgatctgaaa aaaatggtcg ccgaccgaga gttccgtagc
    39661 gatctctatt accgcctgaa cgtattcccg attcacctgc cgccactacg cgagcgtccg
    39721 gaagatattc cgctgctggc gaaagccttt accttcaaaa ttgcccgtcg tctggggcgc
    39781 aatatcgaca gcattcctgc cgagacgctg cgcaccttga gcaacatgga gtggccgggt
    39841 aacgtacgcg aactggaaaa cgtcattgag cgcgcggtat tgctaacacg cggtaacgtg
    39901 ctacagctgt cattgccaga tattgtttta ccggaacctg aaacgccgcc tgccgcaacg
    39961 gttgtcgccc aggagggcga agatgaatat cagttgattg tgcgcgtgct gaaagaaacc
    40021 aacggcgtgg ttgccgggcc taaaggcgct gcgcaacgtc tggggctgaa acgcacgacc
    40081 ctgctgtcac ggatgaagcg gctgggaatt gataaatcgg cattgattta actgcaaatt
    40141 gccggacaga tctgcctgtc cggcatacta ttcacgaggt tttttcggac gatatttttc
    40201 cggcagttct ggcaccggac gcttgtcatc gatgagatga cgcacggtta agatcggatg
    40261 acgccacagc attctcggcc cggcccaacg cataatctgt ttcatctctt cacgctttgc
    40321 aggctggtaa cagtgcaccg gacactgctt acaggctggt ttctcttcgc cgaacacaca
    40381 tttatccagc cgcttttgcg cgtaaacaaa caacgcctcg taatgctccg gctccgctga
    40441 cgcctgcggg catttcgctt gataaagatc gatcattttt ttaatcgtca gtttttcacg
    40501 agagatacgc ttgccggaca tgctgcctcc atactcttaa ggtgtattta tattacaact
    40561 taatttgaaa ggggactatg gctctgaagg aggaggttta ttcaaccgac gcaattttgc
    40621 gtacttgctt cataagcatc acgcaaaagc tgcaaaacag catctttccc ggaaccagca
    40681 tcaagaactc gcagttcgct tcttcccctg aaatgattaa ctccggtatc atgtgcgcct
    40741 tatgtgatta caacgaaaat aaaaaccatc acaccccatt taatatcagg gaaccggaca
    40801 taaccccatg agtgcaatag aaaatttcga cgcccatacg cccatgatgc agcagtatct
    40861 caggctgaaa gcccagcatc ccgagatcct gctgttttac cggatgggtg atttttatga
    40921 actgttttat gacgacgcaa aacgcgcgtc gcaactgctg gatatttcac tgaccaaacg
    40981 cggtgcttcg gcgggagagc cgatcccgat ggcggggatt ccctaccatg cggtggaaaa
    41041 ctatctcgcc aaactggtga atcagggaga gtccgttgcc atctgcgaac aaattggcga
    41101 tccggcgacc agcaaaggtc cggttgagcg caaagttgtg cgtatcgtta cgccaggcac
    41161 catcagcgat gaagccctgt tacaggagcg tcaggacaac ctgctggcgg ctatctggca
    41221 ggacagcaaa ggtttcggct acgcgacgct ggatatcagc tccgggcgtt ttcgcctgag
    41281 cgaaccggcc gaccgcgaaa cgatggcggc agagctgcaa cgcactaatc cggcggaact
    41341 gctgtatgca gaagatttcg ccgagatgtc gctgattgaa ggccgtcgcg gcctgcgccg
    41401 tcgcccgctg tgggagtttg aaatcgacac cgcgcgccag cagttgaatc tgcaatttgg
    41461 cacccgcgat ctggtcggtt ttggcgtcga gaacgcgccg cgcggacttt gtgctgccgg
    41521 ttgtctgttg cagtatgcga aagataccca acgcacgacc ctgccgcata ttcgttctat
    41581 caccatggaa cgtgagcagg acagcatcat tatggatgcc gcgacacgtc gtaacctgga
    41641 aattactcag aacctggcgg gcggtgcgga aaatacgctg gcttctgtgc tcgactgcac
    41701 cgtcacgccg atgggtagtc gtatgctgaa acgctggctg catatgccag tgcgcgatac
    41761 ccgcgtgttg cttgagcgcc agcaaactat tggcgcattg caggatttca ccgccgagtt
    41821 gcagccggta ctgcgtcagg tcggcgacct ggaacgtatt ctggcacgtc tggctttacg
    41881 aactgctcgc ccacgcgatc tggcccgtat gcgccacgct ttccagcaac tgccggagct
    41941 gcgtgcgcag ttagaaactg tcgatagtgc accggtacag gcgctacgtg agaagatggg
    42001 cgagtttgcc gagctgcgcg atctgctgga gcgagcaatc atcgacacac cgccagtgct
    42061 ggtacgcgac ggtggtgtta tcgcatcggg ctataacgaa gagctggatg agtggcgcgc
    42121 gctggctgac ggcgcgaccg attatctgga gcgtctggaa gtccgcgagc gtgaacgtac
    42181 cggcctggac acgctgaaag ttggctttaa tgcggtgcac ggctactaca ttcaaatcag
    42241 ccgtgggcaa agccatctgg cacccatcaa ctacatgcgt cgccagacgc tgaaaaacgc
    42301 cgagcgctac atcattccag agctaaaaga gtacgaagat aaagttctca cctcaaaagg
    42361 caaagcactg gcactggaaa aacagcttta tgaagagctg ttcgacctgc tgttgccgca
    42421 tctggaagcg ttgcaacaga gcgcgagcgc gctggcggaa ctcgacgtgc tggttaacct
    42481 ggcggaacgg gcctataccc tgaactacac ctgcccgacc ttcattgata aaccgggcat
    42541 tcgcattacc gaaggtcgcc atccggtagt tgaacaagta ctgaatgagc catttatcgc
    42601 taacccgctg aatctgtcgc cgcagcgccg tatgttgatt attaccggcc cgaacatggg
    42661 cggtaaaagt acctatatgc gccagaccgc actgattgcg ctgatggcct acatcggcag
    42721 ctatgtaccg gcacaaaaag tcgagattgg acctatcgat cgcatcttta cccgcgtagg
    42781 cgcggcagat gacctggctt ccgggcgctc aacctttatg gtggagatga ctgaaaccgc
    42841 caatatttta cataacgcca ccgaatacag tctggtgtta atggatgaga tcgggcgtgg
    42901 aacgtccacc tacgatggtc tgtcgctggc gtgggcgtgc gcagaaaatc tggcgaataa
    42961 gattaaggca ttgacgctgt ttgccaccca ctatttcgag ctgacccagc taccggagaa
    43021 aatggaaggc gtcgccaacg tgcatctcga tgcgctggag cacggagaca ccattgcctt
    43081 tatgcatagc gtgcaggatg gcgcggcgag caaaagctac ggcctggcgg ttgcagctct
    43141 ggcaggcgtg ccaaaagagg tgattaagcg cgcacggcaa aaactgcgtg agctggaaag
    43201 catttcgccg aacgccgccg ctacgcaagt ggatggtacg caaatgtctt tgctgtcagt
    43261 gccagaagaa acttcgcctg cggtcgaagc actggaaaat ctcgacccgg attcactgac
    43321 tccgcgtcag gcgctggagt ggatttatcg cttgaagagt ctggtgtaat aacaattccc
    43381 gatagtcttt tgctatcggg aatattaacg acaactgacg aatcaaataa aaataccctg
    43441 tataatagga aagcttattt tacagggtaa aaccatgcca tctacacgct atcaaaaaat
    43501 caatacccat cactatcgcc atatatgggt cgttggtgat attcatggtg aatatcagtt
    43561 attacaatcc cgcttacatc aactctcttt tttccccgaa accgacttac ttatttctgt
    43621 cggcgataat attgatcgtg ggccggagag tcttgacgtc ctgcgcctgc taaaccagcc
    43681 ctggtttatc tccgttaaag gcaaccacga agcaatggcg ctggatgcgt tcgcgaccgg
    43741 cgatggcaat atgtggcttg ccagcggtgg tgactggttt ttcgatttaa atgattcaga
    43801 gcaaaaagaa gctacagatc tgttgctgaa attccatcac cttccacata ttattgaaat
    43861 cactaacgac aacataaaat atgtcatcgc acatgcagat tatccgggga gtgaatatct
    43921 ctttggtaaa gaaatagcgg agagcgaatt actctggcct gttgatcgtg tgcagaaatc
    43981 gcttaatggc gagttacaac aaataaacgg cgctgattat tttatatttg gacatatgat
    44041 gtttgataac attcagacgt tcgctaacca gatttatatt gataccggca cgccgaaaag
    44101 cgggcggctg tcattttata aaataaagta gtctcatgct tcttctgtga agcatgagta
    44161 accaaatgtt attgcaggcc attattcatt tttcgctacc agcaaagaga gatcctgctt
    44221 cgccagcgcg cgactggcac tctccggcaa accgtcgtct gtaataatct gatcaaactc
    44281 gcttaatggt aacgccagcc atgtcgccac ctgaccatat ttcgtcgcat cacagaccaa
    44341 aactcgctgg cggctggcac tggcaatcgc ccgtttcacc gtgactttat cttccgctgg
    44401 cgtagaaatc ccccgcacac tccatgacga tgcagaaata aaagcctgat caatcatcag
    44461 gctgcgcagc atggtcgcag cggcttcccc gacacaggaa cggttttccc gacacactgc
    44521 accgccagtg tgaataattg tgcaattact gttgtcgagc aagtagttcg caataacgaa
    44581 atcgtttgtg accacagtca gtgactccat gtgaatcaga tgctgtgcta tcgctaacgt
    44641 ggtcgttccc gcatccagat agatacaact tcccggctga acaagacttg ccgccagctt
    44701 gccaatagcc gctttttgcg tcattgccag cgcagttttt acctgatgag aaggttcatg
    44761 cgccacgcgt cccggagact ggacgcctcc ggacaccagc acaacggctc cctgctgctc
    44821 cagtttttgt aaatcccgac gaatggtcat atgtgacaca ttcattctgt ccgttagttc
    44881 agcaatactg acaatgcctt tttcagctac catctcaagg atgatttggc gacgctctac
    44941 gggtatcaac ttttgctcct tcctttgtcc tgctgacatt ctacgctatt tgcctgcgaa
    45001 acgtgcgcgg cgcaactaac gcttagttca cataaaataa cacacaatgt taatttatgt
    45061 gaatcagctc accatatcgt tatcttccag cgcttatatt cacaatatca aacaaaatat
    45121 cacttaaatt aacaaggaga gcagatgaaa acgggatctg agtttcatgt cggtatcgtt
    45181 ggcttagggt caatgggaat gggagcagca ctgtcatgtg tccgcgcagg tctttctacc
    45241 tggggcgcag acctgaacag caatgcctgc gctacgttga aagaggcagg tgcttgcggg
    45301 gtttctgata acgccgcgac gtttgccgaa aaactggacg ctctgctggt gctggtggtc
    45361 aatgcggccc aggttaaaca ggtgctgttt ggtgaaacag gcgttgcaca acatctgaaa
    45421 cccggtacgg cagtaatggt ttcttccact atcgctagtg ctgatgcgca agaaattgct
    45481 accgctctgg ctggattcga tctggaaatg ctggatgcgc cagtttctgg tggtgcagta
    45541 aaagccgcta acggtgaaat gactgtcatg gcctccggta gcgatattgc ctttgaacga
    45601 ctggcacccg tgctggaagc cgttgccgga aaagtttatc gcataggtgc agaaccggga
    45661 ctaggttcga ccgtaaaaat tattcaccag ttgttagcgg gcgtacatat tgctgccgga
    45721 gccgaagcga tggcacttgc agcccgtgcg gggatcccgc tggatgtgat gtatgacgtc
    45781 gtgaccaatg ccgccggaaa ttcctggatg ttcgaaaacc ggatgcgtca tgtggtggat
    45841 ggcgattaca ccccgcattc agccgtcgat atttttgtta aggatcttgg tctggttgcc
    45901 gatacagcca aagccctgca cttcccgctg ccattggcct caacagcatt gaatatgttc
    45961 accagcgcca gtaacgcagg ttacgggaaa gaagacgata gcgcagttat caagattttc
    46021 tctggcatca ctctaccggg agcgaaatca tgatcaagat tggcgttatc gccgatgatt
    46081 ttaccggcgc gacggatatc gccagttttc tggtggaaaa cggtctacca acggtacaaa
    46141 ttaacggtgt tccaacaggt aaaatgccgg aagcaatcga cgcactggtg atcagcctga
    46201 aaacgcgctc ctgtccggtg gttgaagcca cacagcaatc gctggcggct ctgagctggt
    46261 tgcaacagca aggttgcaaa cagatctatt tcaaatactg ctctactttc gacagtacgg
    46321 cgaaaggtaa tatcggcccg gttaccgatg cattaatgga tgctctcgac acgccgttta
    46381 cggtcttctc tccggccctg ccggtcaacg gacgtacggt ttatcagggg tatttgttcg
    46441 taatgaatca actgctggcc gaatccggga tgcgccatca tccggtaaat cccatgaccg
    46501 acagctatct tccccgtctg gttgaagcgc aatccacagg gcgctgcggc gtcgtttcgg
    46561 cacatgtttt cgaacaaggt gtggatgccg ttcgtcaaga gctggctcgc ttacagcaag
    46621 agggctaccg ctacgcggtg cttgatgcgc tgaccgaaca ccatctggaa attcagggag
    46681 aagccttgcg cgatgcccca ctggtaacgg gcggttctgg tctggcgatt ggcctggccc
    46741 ggcagtgggc gcaagaaaac ggtaaccagg ctcgcgaagc agggcatccg ctcgctgggc
    46801 gcggcgtagt gctctccggt tcatgctctc aaatgaccaa ccgccaggtg gcacattacc
    46861 gtcaaattgc accagcccgt gaagttgatg tggcacgctg cctctcaacc gaaactctgg
    46921 ccgcttatgc acacgaactg gcagagtggg ttctgggcca ggaaagtgta cttgctccac
    46981 tggtttttgc caccgccagc actgacgcat tggcagcaat tcaacagcaa tacggtgcac
    47041 aaaaagccag tcaggcagta gaaactctat tttctcaact agcggcgcgg ttagcagcgg
    47101 aaggcgtgac acgctttatt gtcgcaggcg gtgagacctc cggcgtagtc acacagagcc
    47161 tgggcataaa agggtttcat attggcccaa ccatttcccc cggcgtgccg tgggtaaacg
    47221 cactggataa gcctgtctca ctcgccctta aatctggcaa cttcggtgat gaagcctttt
    47281 tttcacgagc ccaaagagag tttttatcat gagcgatttc gcaaaagtag agcagtcttt
    47341 gcgagaggag atgacgcgga ttgccagttc attctttcag cgcggctatg caaccggttc
    47401 ggctggcaat ctgtcgctgc ttttacctga cgggaattta ctggcgacac cgacaggttc
    47461 atgcctgggc aatctcgatc cgcagcggct ttccaaagtc gccgcggatg gcgaatggtt
    47521 aagtggtgac aaaccctcga aagaggtgct ctttcatctg gcgctgtatc gcaacaatcc
    47581 gcgctgtaaa gcggtggtgc atttgcacag cacatggtcg acggcgcttt cctgcctgca
    47641 agggctggac agcagcaacg ttattcgtcc gttcacacca tacgtggtga tgcggatggg
    47701 aaatgtcccg ctggtgcctt attaccgacc gggcgataaa cgcatcgcac aggatctggc
    47761 ggaactggca gcagacaatc aggctttttt actggcaaat catggcccag tggtttgcgg
    47821 tgaaagcctg caagaagccg ccaacaatat ggaagagctg gaggaaacgg caaagctgat
    47881 ttttattctc ggtgaccgcc cgatccgtta tctgaccgca ggtgaaattg cggaattaag
    47941 gagttaatgc aatgcctcgt tttgcagcta atttatccat gatgttcacc gaagtgcctt
    48001 ttattgaacg cttcgccgca gcgcgaaaag ccgggttcga tgctgtggaa tttctgtttc
    48061 cctatgacta ctccaccctg caaatccaaa agcaactgga gcaaaaccat ctgacactgg
    48121 cgctgttcaa taccgctccc ggagatatta atgcggggga atggggatta tccgcccttc
    48181 ccggacgtga gcacgaagca cacgcagaca tcgacctggc actggaatat gcattggcgc
    48241 tcaattgtga acaagtccat gtgatggcag gcgtcgtgcc cgctggcgaa gacgctgaac
    48301 ggtaccgggc agtatttatc gataatctcc gctacgctgc cgaccgcttt gcgctacacg
    48361 gcaagcgaat attagttgaa gcactcagcc ccgacgttaa gcctcattat ctcttctcca
    48421 gccagtatca ggcactggct attgttgaag aggttgcgcg agataacgtg tttattcaac
    48481 tagatacttt tcatgcgcaa aaagtagatg gcaacctgac gcatttaatc cgcgactacg
    48541 ctgggaaata tgcgcacgta caaattgccg gactacctga tcgacatgaa ccggacgatg
    48601 gagaaatcaa ctatccgtgg ctgttccgcc tgttcgatga ggtgggatat cagggctgga
    48661 tcggttgtga atataaacct cgtggcctca ccgaagaagg gcttggctgg tttgacgcct
    48721 ggcgctaatt cgttaaatcc ctaattacaa cgtacccata catcccccca tctacggatg
    48781 gggtaggtta actataactt tcagacaggg tttcccatgt ccacaattac attgttatgc
    48841 attgcgttag ctggcgtaat catgctgttg ctgctggtca tcaaggcaaa ggtacaacca
    48901 ttcgttgctc tgctcctcgt cagcctgtta gtcgcacttg cggcgggtat accggcgggc
    48961 gaagtgggta aagtgatgat cgccgggatg ggcggtgtgc ttggttccgt cactattatt
    49021 attggtctgg gcgctatgct ggggcgtatg atcgaacact ctggtggtgc agagtcactg
    49081 gctaattatt tcagtcgcaa gttaggtgac aaacgaacta tcgctgcgct gactctggca
    49141 gcgttcttcc tcggtattcc cgtcttcttt gatgtcggct ttattattct tgcgccaatc
    49201 atttacggtt ttgccaaggt tgccaaaata tcgccactca aatttggcct gcctgtcgct
    49261 ggaatcatgc taactgttca tgtggcggta ccgccgcatc caggccctgt cgccgcagcg
    49321 gggttactcc acgcagacat cggctggcta accatcatcg gtattgcgat ttctattccc
    49381 gtagggattg ttggctactt tgcagcgaaa ataatcaata cgcgccaata tgcgatgtcg
    49441 gtggaagtac tggaacagat gcaactggct ccggccagtg aggaaggcgc aacaaaatta
    49501 agcgataaaa taaatccacc gggcgtcgcg ctggtcacct cgctaattgt tattcctatc
    49561 gcgattatca tggcgggtac ggtttccgca acactgatgc cgccttcgca tcccctgctt
    49621 ggtacgctac agctgatcgg ctcaccaatg gtagctctaa tgattgcgct ggtgctggca
    49681 ttctggttat tggctttacg tcgcggctgg agcttacaac acaccagcga cattatgggc
    49741 tcagcccttc ccactgcggc ggtagtgatt ttggttactg gtgctggagg ggtatttggc
    49801 aaagtgctgg tggaatcggg cgtcggcaaa gcccttgcca atatgctaca aatgattgac
    49861 ctgcctctgt taccagccgc atttattatt tcactggctc tgcgtgcatc gcaggggtca
    49921 gcaaccgtag caatcctgac aaccggcggg ttactctcag aagcggtgat gggattgaat
    49981 ccgattcagt gcgtattggt gacgctggca gcctgctttg gtgggcttgg cgcgtcacat
    50041 attaatgact cagggttctg gattgtgacc aaatatctgg ggttgtcggt agcagacggt
    50101 ctgaaaacct ggacagtgtt aacgaccatt ctcggtttta ccggattctt aattacctgg
    50161 tgcgtatggg cggtaatttg accttaataa aaaggtccga tgggcatcgg accttttatt
    50221 taacgcttac cttccaccag cagcggcggg atgctcggca ccattggcgc gtcatcaata
    50281 tctttctgcg tcatgcggaa cgcttcggga taatgttcgc ggctggtacg gcgcagcgat
    50341 tcggtatcgc gccaggtata aaggcaatgc tggcactgat ataccgtcca gacatctttc
    50401 accggcgatt tcgccatcac ttcaatctgt tcatcggcac aacgtggaca aatcatcttc
    50461 ttctccttat ttacgtgcag ccagcatagc ggtcagtttt tcagcccagg ctttggtttc
    50521 cggtaaatcc accaccggtt ggctgtagtg accacggttg tccggggcga caggcgtagt
    50581 ggcgtcgata atcagcttgt cggtgatccc cgcagggctt gagcctggat cgagttccag
    50641 cacggacata ttcggcaact gcaccaaatc ccctgccggg ttcactttcg aggagagcgc
    50701 ccacatcacc tgcggcaggt tgaacgggtc aacgtcttca tcgaccataa tcaccatctt
    50761 cacgtagccc agaccatgcg gcgtggtcat tgcgcgcagg cccaccgcgc gggcaaagcc
    50821 gccgtagcgt tttttggtgg agataatcgc cagcaggcca tgggtgtaca tggcgtttac
    50881 cgcctgcact tcagggaact cggctttcag ctgctgatac agcggcacgc aggtggctgg
    50941 ccccatcagg tagtcgattt cggtccacgg cataccgagg tacagcgatt cgaaaatcgg
    51001 cctggtgcgg taagagactt tatcgatgcg caccacggtc atgttacgcc cgccggagta
    51061 gtgcccggta aactcaccga acggcccttc gatttcgcgt ttacggcttt cgatgacccc
    51121 ttcgaggatc acttctgaac cccacggcac atcaaaaccg gtcaacgggg cggtggcgat
    51181 cgggtacggg ctttcacgca gcgcgcctgc catttcgtac tcggactgat catatttcag
    51241 cggcgtggcc cccatcagcg tgatgatcgg atcgttaccg agcgtgatcg caatcggcag
    51301 atcttcaccg cgctcttctg ctttatgcag atgcagggcg atatcgtgca tcggcaccgg
    51361 ttgcaggccg agcttacgct tgcccttcac ttccatgcgg tagatgccga cgttctgctt
    51421 gccgaagtta tccgggtcga gcggatcgcg ggaaaccacg cacgctttgt cgagatagaa
    51481 accgccatcg ccatcgttta aacgaaacag cggcaggata tcgaacaggt tgatctcgtc
    51541 gccatcaacg gtgttctgcg cccaggctgg attggcgcgg cgctccgggg caatcgggaa
    51601 gttatcccag cggcggataa actcatcaat ctgcttttta accggggcat ttggcggcag
    51661 gccgagggaa atcgcgtggt tctgccagga accgatggtg ttcatcgcca cgcgggcatc
    51721 ggtaaagccg cgaatattat caaaccacag cgcgggcgcg ccgtcgccga tacgcccggt
    51781 ggcgttagct gctgctgcca gatccggctc ggcgttcact tcttcgctga ttttcagtaa
    51841 ctggccgtgg tcatcaagcg cctgtaaaaa gctgcgtaaa tcatcaaatg ccattattca
    51901 ttctcctgag aaaaattccg ggcctgcggc aatccttgcc agcgcctggc gtggggatgt
    51961 tcgaggccaa attgatccag cacgcgggct accacatggt ggacaatgtc atctaccgtt
    52021 tcgggatggt tataaaaggc aggcatcggc ggcaccatcg ccacgcccat gcgtgaaagt
    52081 gcgagcatat tttcgagatg gatggtgcta agcggcattt cacgcggcac cagcaccagt
    52141 ttgcggcctt ctttgagcac gacgtccgcc gcgcgcccta ccaggccatc ggcgtaacca
    52201 gcgcggatac cggcgagcgt tttcatactg cacggaataa cgatcatgcc gtcggtacga
    52261 aaagaaccgg atgagatgat cgccgcctga tccgccgggt tatggctgaa gtctgcgagg
    52321 gcagcaacat cgcgagcgct gtaaggcgtt tccagttcaa tggtggtttt cgcccacttc
    52381 gacatcacca gatgagtctc gacattcggc atctcccgca gcgcttgcag taatgccaca
    52441 ccaagaggcg caccggtagc ccctgtcatc ccgacgatca gtttcattgc ttatcctctt
    52501 aattgttcgt atacgaacgt tattattaac atactccttc atttccacag cggcaaggat
    52561 tgaaaagtaa caacatcttc ttcgtcacta aaaaaccgct atgatagcga cagagaatta
    52621 gcccggagtt tacatggcgt tacgaaataa agcgttccat cagttacgac agctttttca
    52681 gcagcacacg gctcgctggc agcacgagtt acctgacctg accaaaccac agtacgcggt
    52741 gatgcgcgcc attgctgata aacctggtat cgaacaggtg gcactgatag aagcagctgt
    52801 cagcaccaaa gcaacgttag cagaaatgtt ggcaagaatg gaaaatcgtg gcttagtcag
    52861 acgtgaacat gaccccgctg ataagcggcg acgctttgtc tggttgactg ccgaaggcga
    52921 aaagatactt gctgcggcga tacccattgg tgacagcgtg gatgaggaat ttttggggcg
    52981 tttgagtgga gaagagcagg agctgtttat gcagctggtg cgcaagatga tgaacgcata
    53041 gggggcaaat ttccggatgc gacgctatcg cgtcttatcc agactacaaa tacaccgact
    53101 caccaaccga aagaaaaaag gccagcctcg cttgagactg gcctttctga cagatgctta
    53161 cttactcgcg gaacagcgct tcgatattca gcccctgcgt ttgcaggatt tcgcgcaaac
    53221 ggcgcaggcc ttcaacctga atctggcgaa cacgttcacg ggtgaggcca atttcacgac
    53281 ctacatcttc cagtgttgcc gcttcgtacc ccagcaaacc gaatcgacgt gccagcactt
    53341 cacgctgttt ggcgttcagc tcgaacagcc atttgacgat gctctgcttc atatcgtcat
    53401 cttgcgtggt atcttccgga ccgttctctt tttcatcggc caggatgtcc agcaacgctt
    53461 tttcggaatc accacccagc ggggtgtcta ccgaggtaat gcgctcgtta agacgaagca
    53521 tacggctgac gtcatcaact ggcttatcca gttgctctgc gatctcttcc gcacttggtt
    53581 catggtccag cttatgggac aactcacgtg cggttcgcag gtaaacgttc agctccttta
    53641 cgatgtgaat cggcaaacga atagtacggg tttggttcat aatcgcccgt tcaatcgtct
    53701 ggcgaatcca ccaggttgcg tatgttgaga agcggaaacc acgttccggg tcaaacttct
    53761 ctaccgcgcg gatcagcccc aggttgccct cttcgataag gtccagcaac gccagaccac
    53821 gattgccata acggcgggca atttttacca ccagacgcaa gttactctcg atcatccggc
    53881 ggcgagaggc gacatctcca cgcagtgcgc gacgcgcaaa ataaacttct tcttcggccg
    53941 ttaacagtgg tgaataacca atctcaccaa ggtaaagctg agtcgcgtcc aacacacgct
    54001 gtgtggctcc ctgcgataac agttcctctt cggccaaatc gttatcactg ggttcctctt
    54061 ctactaaggc cttttcgtca aaaacctcaa ctccgttctc atcaaattcc gcatcttcat
    54121 ttaaatcatg aactttcagc gtattctgac tcataaggtg gctcctaccc gtgatccctt
    54181 gacggaacat tcaagcaaaa gcctggttcc gccgatttat cgctgcggca aataacgcag
    54241 cgggtttacg gatttcccct tgtaacgaat ttcaaaatgc aagcgtgttg aactggttcc
    54301 ggtgctaccc atggttgcta ttttttgccc cgccttcact tcttgttgtt cccggaccag
    54361 cattgtgtcg ttatgggcgt aggcactcag gtaatcatca ttatgtttga tgataatcag
    54421 attaccgtag ccgcgcagcg cgttaccggc ataaacaacg cggccatctg cggtcgcgat
    54481 aattgcctgt cctttgctgc ctgcgatatc aatccccttg ttgcccccct cagaagcgcc
    54541 aaaggtttcg atcactttgc cctcagtcgg ccagcgccag gtggagatag gcgtactggt
    54601 tgatgtactg ctgacagttg gctcggttgt gcttgctgtt ggtaccgtta caggcgctgt
    54661 gaccgtggtc gcagctggct tgttgttcgg caacattttg ttagcactct gttcacccga
    54721 agattcagaa tacgtaattg tcggttgcga cgcaacagca acggtggaat tttgtgcagg
    54781 cttgatcaca actccttgct ctgctgcgtc ggcctgggta atggcatttc cgccagtgat
    54841 tggcgtaccg gaagcattac ccacctgcaa ggtctgacca acgttcagcg cgtatggtgc
    54901 ctgaatattg ttgcgctgag caaggtcacg gaaatcgttg ccagtaatcc aggcgatata
    54961 gaaaagtgtg tcgccttttt tcacggtata ggtactgccg ctataactgc ctttcggaat
    55021 gttcccatac tgacggttat agacgatgcg tccgttttcc atctgtaccg gctgctgagc
    55081 tactggctgc actggctgga tttgcggttg ttgagtagcc tgaatttgtg gctgctgtac
    55141 cggctgaatt tgcggttgct gggctgtaga cgtcgtcccc attttcggcg gcggcgtaat
    55201 caacatacca gaattggtat ttgcaggcgc attgccatta acggagctga ccggtgccgg
    55261 tggatttgaa gtgtcagaac agcctgccag ccatagcgaa accagtgaca aagccgcaat
    55321 gcggcgaacg gtgaattttg ggcttcccgc gctcatttat cccccaggaa aaattggtta
    55381 ataaccagtg acataattac cgtgcaaggc accctactga acactggaaa agatgttcac
    55441 gatacgctga cctgcggcaa aataaccagg aaaaatccag gtatttcctc acgttttaag
    55501 ccagctcacc cttcactaaa ggtacaaagc gcacggcctc cacggtatcg ataataaatt
    55561 cgcctccccg acgacgcacc cgtttcaaat actggtgctc ctcccctacg ggtaagacga
    55621 gaatcccgcc ttcgtccagc tgcgtcatta gcgcagttgg aatttccggc ggtgccgccg
    55681 taacaatgat agcgtcaaac ggcgcgcgtg cctgccaacc ttgccatcca tcgccatgac
    55741 gggttgaaac attatgtaaa tcaagatttt tcaggcggcg acgcgcctgc cactgcaaac
    55801 ctttaatccg ttcgaccgag caaacatgct ggacaagatg cgccaggatt gccgtttgat
    55861 atcccgaacc agtgccaatt tccagcaccc gcgactgcgg cgtcagctcg agtaattcgg
    55921 tcattcgcgc caccatatac ggctgcgaaa ttgtctgccc ctgacctatc ggcaaggcga
    55981 tattgtccca ggctttttgt tcaaacgctt catcaacgaa tttttcacgc ggcacggcgg
    56041 caagtgcatt cagcacctgc tcatcctgaa taccttgcgc acgtaattga tccagaagtg
    56101 cttgtacgcg tctgcttacc attgcgtgcc aactcccacg ctgtttaacc agtctgaaac
    56161 cacatcttgc gcgctatgcg cagttaaatc cacatgcagc ggcgtgatgg agacatagcc
    56221 ctcatctacc gcagcaaaat cggtccccgg accggcatca catttaccgc ccggcgggcc
    56281 aatccagtac agcgtattgc cgcgcggatc ttgctgcggg atcacctgat ctgccggatg
    56341 tcgtgtaccg cagcgcgtca cgcgaatacc tttgatttga tccaagggta aatccggaac
    56401 gttaatatta agaatacgcc cggtgcgcaa cggctcttta cacagtgcgc gcaaaattga
    56461 acaggttacc gccgcggcag tgtcgtaatg tttatgcccg tcaagcgaga cggcaagcgc
    56521 cggaaaacct aaatgacggc cttccatcgc ggcggctacc gtaccggaat aaataacatc
    56581 atcccccaga ttcggcccgg cgttaattcc ggacacaaca atgtccgggc gcggacgcat
    56641 cagagcattc acgccaagat agacgcaatc ggtcggggtt cccatttgca cagcaatatc
    56701 accattttca aaggtaaacg tgcgcaggga ggattccagt gtcagagaat ttgaagcgcc
    56761 gctgcggtta cgatcggggg cgaccacctg aacgtcagca aactcacgca aggctttcgc
    56821 cagcgtttgt ataccgggtg catgtacccc gtcatcatta ctcagcaata tgcgcataat
    56881 cacctgttgt gttgataagt tccctgacaa cgctggttgc aaaactaccc gccggaagcc
    56941 agaaacggat ctctacggtg acgtcatccc accaattcca gcttaattgt tgcggataca
    57001 gcagcatcgc tctgcgcgcg gcttcaactt tttcgcgcac cagtaaagct tgtaattcag
    57061 tttctgcggc gacagctgct tgttcgaatg ccagcgcttc acgctgagtt ccccattcgc
    57121 cactgcccgg caatgcggcg gttatcatca gctctttatc gttaacgcga cgctgtaatt
    57181 ccgccagttc ttcggtggtt gcgacaaacc agctaccacg tccggctaat tgtagcgcat
    57241 cgccgtcaac aacttgatta acgtctgctt ttttgaggcg ctcagcaaca atctgattaa
    57301 acaacgcact gcgggctgcc gacaaccaaa aactccgttt attgcgatcg cgcaccggag
    57361 tattggtttg cgcccagcgc tgcgccccct gcaagttgct accgccaatc ccaaaacgtt
    57421 gggcaccgaa gtagttcggt acacctttta cgcaaatatc gttcagacgt tgttcaacgt
    57481 catcgcgatt gctcacttcg cgcagaacca gggtaaaggc gtttcctttc agcgccccta
    57541 aacgcagctt gcgcttgtgc cgcgcatact ccagcacctg gcagccttcc agttgaaagg
    57601 cgctcagatc gggcatttcc ttgcccggca cgcgagcgca taaccactgt tccgtaacag
    57661 catgtttgtc tttttgccca gcgaagctga cttcacgggc atgaattttc aggaatttcg
    57721 ccagtgcatc cgccacaaaa cgggtattgc agccgttttt gaggattcta accagaatat
    57781 gctcaccttc accatcaggc tcaaagccca aatcttccac caccacaaag tcttccggat
    57841 tggctttcag cagcccggtg ccttgcggtt taccgtggag gtaagtgaga ttatcaaact
    57901 caatcatttt gttgccttaa tgagtagcgc caccgcttca caggcaatcc cttccccacg
    57961 tccggtaaat cccagttttt ccgtagtagt ggctttcacg ttaacatcat ccatatggca
    58021 gccgagatct tcggcaataa acacgcgcat ttgtggaatg tgcggcaaca tcttcggtgc
    58081 ctgagcgatg atagtgacat cgacgttgcc aagggtataa cccttcgcct gaatacgacg
    58141 ccaggcttcg cgtagcagct cgcggctatc ggcaccttta aatgccggat cggtatccgg
    58201 gaacagcttg ccgatatccc ccagcgccgc cgcgccaagc aatgcatcgg tcaacgcatg
    58261 gagcgccacg tcgccatcag aatgcgccag caatcctttt tcgtaaggaa tgcgtacgcc
    58321 accaatgata attgggcctt caccgccaaa ggcatgtacg tcaaaaccgt gtccaattcg
    58381 cattatgtat tctcctgatg gatggttcgg gtgaggtaaa actcggccag tgccaaatct
    58441 tccgggcgcg tgactttaat gttatccgca cggccttcga ccaactgagg atggaatccg
    58501 caatattcca gcgccgaggc ttcgtcggta atagtcgcgc cttcatttag agcgcgcgtc
    58561 agacagtcat gtaacagctc acgagggaaa aattgcggcg tcagcgcgtg ccataagccg
    58621 ttgcgatcaa cggtatgagc aatggcattt ttgcccggtt cggcacgttt catagtatcg
    58681 cgcactggtg cggcgaggat cccccccgtg cggctggttt cgctcaacgc caacaatcgc
    58741 gcgaggtcat cctgatgcaa acaaggacga gcggcgtcat gcaccaatac ccactgcgcg
    58801 tcgccagcgg ctttcagacc tgccagcacg gaatcggcac gctcatcacc gccatctaca
    58861 acggtgattt gcggatgatt cgccagagga agttgtgcaa aacggctatc gccaggactt
    58921 atggcaatga cgactcgttt cacccgggga tgcgccagca gcgcatgcac cgagtgttca
    58981 agaatggttt gattaccgat tgagagatat tgcttaggac attccgtttg cattcgacgg
    59041 ccaaatccgg ccgccggaac cacggcgcaa acatccaaat gagtggttgc catgttaatt
    59101 cccgggctga tttatcgatt gttttgcccc gcagactgtg cgcgcttcga cgcgtcaggc
    59161 accagacgat aaaaagtttc gcccggcctg gtcatgctga gttcattacg cgcacgctct
    59221 tcgagcgcct cctggccgcc attgagatcg tcaatttcgg caaaaagttg atcgtttcgc
    59281 gctttaagtt tcgcgtttgt agcttgctgt gccgccacat catcattgac gcgggtatag
    59341 tcatgtatac cgttcttacc gaaccacagc gaatactgta gccagaccag aatagccagc
    59401 aacagcagcg ttagtttacc catcctgccc cctgaaaaac ggcatcatca tcccatgcat
    59461 ccgaagacga ctctacatcc tctgttgggg ataccgcgac aacgcgggca aatgtaccac
    59521 atttgtccat tgttacgtat acccagggcg tgcagaacat aatctcatta ttagttacgg
    59581 tttgaattat gaacagagga gacgggaaag tacaaattag cccagtagcc acataaacag
    59641 tgcgccaaac ataatgccta ctgtcaccag ggtgaaaaca atactgtagc gtagctttcc
    59701 gtccatcaat gaatgcagcg caatccccac cactaccgcg acgggcatca gcgccagaaa
    59761 gaaaggccag gtgtagataa agaagaacag cgtgttagag ccataaatca acatcggcat
    59821 cgccagcgca aataaccagg agataaaacc gaccacggca ccaggcagtg accatgtggt
    59881 ttcttcatcc tcagtaaggc tgtcgttatt tgttagtgta atgttatggc tattacgcat
    59941 atttgatcct gttactttga cgaaccgggc atggaaaccc ggtggtgtct caggatctga
    60001 taatatcgtt ctgtctcaac agatctaata attgctgtac caaatttgtt actaattgtt
    60061 caccattgag atgaatttct gccgattcag gcgcttcgta aacggaatct attcccgtaa
    60121 agttgcgcag ttcaccggca cgcgctttct tatataagcc ttttggatcg cgggcttcgc
    60181 aaatcgccag cggcgtatcg acaaacactt cgataaagcg cccttctcct acgcgttcgc
    60241 gaaccatctg gcgttcggcg cggtgtggcg agataaatgc ggtcagcact accagtccgg
    60301 cttcaaccat caaattcgcc acttcaccga cgcgacggat attctcttta cgatcggtat
    60361 cgctaaaacc gagatcgctg cataatccgt ggcgaacatt atcgccatcc agcagatacg
    60421 tactgacgcc gagtttatgt aacgcctcct ccagcgcccc ggcgaccgtt gatttaccgg
    60481 acccggagag gccggtaaac cacagcacta caccacgatg accgtggtgt agctcgcgtt
    60541 gttgcacagt gaccggatgg ctatgccaga cgacgttttc gtcatgcagc gccattattt
    60601 atcccccagc aaatcgcgcg cgccccagtg cggaaagtgg cgacgaacca gagcattcaa
    60661 ttccagttcg aatgcactga attcagatgg cgcagcagtt gcctggctaa ctggctcgtg
    60721 caccataccg gcacccacgg tcacattgct caggcgatcg ataaaaatca gcccacccgt
    60781 caccggattt tgttgataac gatctaacac cagcggctcg tcaaaagtga gatccacgag
    60841 gccgatccca ttcagtggca ggttttcaac ttcacgctgg gtaaggttat taatatcaac
    60901 ctgatagcga atgccatcaa cacgcgcgcg cgtcttctta ccggcaattt tgatgtcgta
    60961 actctgccct ggagaaagcg gctgttccgc catccatacc acatccaccg acgcgctctg
    61021 caccgccggt aacgcttcgt ctgccgccag cagcagatcg ccacggctga tgtcgatctc
    61081 atccgtcagc accagggtga tcgcttctcc ggcaaaggct tcttcgcgat caccatcaaa
    61141 agtcacgatc cgcgcgacgt ttgattccac accagagggc agcactttga cacgttgccc
    61201 gacttccacg cgaccggatg ccagcgttcc ggcgtaacca cgaaaatcga gattcgggcg
    61261 attaacgtac tgcaccggga agcgcattgg ctgagcatcc accactcgct ggatctccac
    61321 ggtttccagc acttcgagca gtgtcggacc gctgtaccac ggcatacttt cactttgcga
    61381 tgccacgttg tcgccttcca gtgcagagag cggcacaaag cggatatcca gattacccgg
    61441 cagctgcccg gcaaaggtca aataatcttc acgaatacgg gtgaacgtct cttcactgta
    61501 atccaccaga tccattttgt tgatcgccac gaccagatgt ttgctcccca acagtgtgga
    61561 gataaaactg tgacgacggg tttgatcgag cacgccttta cgggcatcga tcagtaagat
    61621 cgccagttca catgtcgatg cgccagtcgc catattgcgg gtgtactgct cgtgccctgg
    61681 ggtgtcggcg ataataaatt tacgcttctc ggtagagaaa tagcggtagg ccacgtcaat
    61741 ggtgatgccc tgttcgcgct cagcttgcag gccgtccacc agcagagcca gatccagctt
    61801 ttcgccctgg gtgccgtgac gcttactgtc gttatgcagc gatgagagct gatcttcgta
    61861 gatttggcgg gtatcgtgca gcagacgacc aatcagagta cttttgccgt catcgacgct
    61921 accacaggtc agaaaacgca gcaggctttt atgttgttgc gcaatcatcc aggcttcgac
    61981 gccgccttca ttggcgattt gttgtgcaag tgcggtgttc atcttaaaaa tacccctgac
    62041 gttttttcag ctccatagac cccgcctggt cgcggtcaat cacgcggccc tgacgttcac
    62101 tggtggtgga aaccagcatc tcttcgatga tttccggcag tgtttgtgca tttgactcca
    62161 ccgcaccggt cagcggccag cagcccagcg tacggaaacg caccatccgt tttttaatca
    62221 cttcgcccgg ttgcaggtcg atacggttgt catcaatcat catcaacata ccgtcgcgtt
    62281 ccagaaccgg acgttccgca gcgagatata gcggaacaat gtcgatattt tccagccaga
    62341 tgtattgcca gatatcctgc tcggtccagt tagagagcgg gaagacgcgg atgctttcgc
    62401 ctttgttaat ttgcccgttg tagttgtgcc acagctccgg gcgctgattt ttcggatccc
    62461 agcgatggaa gcggtcacgg aaagagtaaa ttcgctcttt agcgcgggat ttctcttcgt
    62521 cacggcgcgc accaccgaag gcggcatcaa aaccgtattt gttcagcgcc tgtttcaggc
    62581 cttcagtttt cataatatcg gtatgtttcg cgctgccgtg cacgaatgga ttaatcccca
    62641 tcgccacgcc ttccgggttt ttatgcacca gcagttcgca gccgtaggct ttagcagtac
    62701 gatcgcggaa ctcatacatc tcgcggaatt tccagccggt atcgacatgc agcaacggga
    62761 aaggcagcgt acctggataa aacgccttgc gcgccagatg cagcatgacg ctggaatctt
    62821 taccgataga gtagagcatc accggatttg agaattctgc cgccacctcg cgaataatgt
    62881 ggatgctttc cgcctccagt tgccgcaggt gagtaagtcg tatttgatcc ataaccgttc
    62941 ctttgcaata ccgctatttt cttgccatca gatgtttcga ctatagggag cgtaagagaa
    63001 cgaatgaaat taccaattag aatgagtagt tccttaacgg aataacgatt tggcaaagct
    63061 aatatcaaaa agtgcttaag gcaccggatt tcgggcgttt aggaagattt gaaattgttt
    63121 tagcgcagcg gcagtttcat actatggcgg taaaaaaatt tgcatggtat ttaaggactc
    63181 actatgtttt ccgcattgcg ccaccgtacc gctgccctgg cgctcggcgt atgctttatt
    63241 ctccccgtac acgcctcgtc acctaaacct ggcgattttg ctaatactca ggcacgacat
    63301 attgctactt tctttccggg acgcatgacc ggaactcctg cagaaatgtt atctgccgat
    63361 tatattcgcc aacagtttca gcaaatgggt tatcgcagtg atattcggac atttaatagt
    63421 cggtatattt ataccgcccg cgataatcgc aagagctggc ataacgtgac gggaagtacg
    63481 gtgattgccg ctcatgaagg caaagcgccg cagcagatca tcattatggc gcatctggat
    63541 acttacgccc cgctgagcga tgctgacgcc gatgccaatc tcggcgggct tacgttacaa
    63601 ggaatggatg ataacgccgc aggtttaggt gtcatgctgg aattggcaga acgcctgaaa
    63661 aatacgccta ccgagtatgg tattcgattt gtggcgacca gcggcgaaga ggaagggaaa
    63721 ttaggcgcag agaatttact caagcggatg agtgacaccg aaaagaaaaa tacgctgctg
    63781 gtgattaatc tcgataactt aattgttggc gataaattgt atttcaacag cggtgtaaaa
    63841 acccctgaag cagtaaggaa attaacgcgc gacagggcgc tggcaattgc gcgtagtcat
    63901 ggaattgccg caacgaccaa tccgggtttg aataaaaatt atccgaaagg cactgggtgt
    63961 tgtaatgacg cagaaatatt cgacaaagcg ggcattgctg tactttcggt ggaagcgact
    64021 aactggaatc ttgggaataa ggatggttat cagcaacgcg caaaaacacc tgccttcccg
    64081 gcgggaaata gctggcatga cgtaagactg gataatcaac aacatattga taaggcactt
    64141 cctggaagaa tagaacgtcg ctgccgtgat gttatgcgga taatgctacc gctggtgaag
    64201 gagctggcga aggcgtcttg atgggtttga aaatgggagc tgggagttct accgcagggg
    64261 cggggaattc taagtgatat ccatcatcgc atccagtgcg cccggtttat ccccgctgac
    64321 gcggggaaca caaaaccaaa cttctccata aattccatag ccgcggttta tccccgctgg
    64381 cgcggggaac actcttcgcg ggtaatcaat gatgattcag tttccggttt atccccgctg
    64441 gcgcggggaa cacgaacgca atatcacggc gttctgcggc ctcggcggtt tatccccgct
    64501 ggcgcgggga acaccaaaat attacgagct tcgtcaggcc atggaccggt ttatccccgc
    64561 tggcgcgggg aacacgtcgg gccgggtaag tgaacct
//
LOCUS       DAZGLJ010000005        58563 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000005 DAZGLJ010000000
VERSION     DAZGLJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.005"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..479)
                     /gene="folK"
                     /locus_tag="ACHK49_000279"
     CDS             complement(<1..479)
                     /gene="folK"
                     /locus_tag="ACHK49_000279"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO9260979.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(476..1873)
                     /gene="pcnB"
                     /locus_tag="ACHK49_000280"
     CDS             complement(476..1873)
                     /gene="pcnB"
                     /locus_tag="ACHK49_000280"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO9260980.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1933..2829)
                     /gene="gluQRS"
                     /locus_tag="ACHK49_000281"
     CDS             complement(1933..2829)
                     /gene="gluQRS"
                     /locus_tag="ACHK49_000281"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO9260981.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2896..3351)
                     /gene="dksA"
                     /locus_tag="ACHK49_000282"
     CDS             complement(2896..3351)
                     /gene="dksA"
                     /locus_tag="ACHK49_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO9260982.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3529..4233)
                     /gene="sfsA"
                     /locus_tag="ACHK49_000283"
     CDS             complement(3529..4233)
                     /gene="sfsA"
                     /locus_tag="ACHK49_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO9260983.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4248..4778)
                     /gene="thpR"
                     /locus_tag="ACHK49_000284"
     CDS             complement(4248..4778)
                     /gene="thpR"
                     /locus_tag="ACHK49_000284"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO9260984.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4852..7281
                     /gene="hrpB"
                     /locus_tag="ACHK49_000285"
     CDS             4852..7281
                     /gene="hrpB"
                     /locus_tag="ACHK49_000285"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO9260985.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKDLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7413..9947
                     /gene="mrcB"
                     /locus_tag="ACHK49_000286"
     CDS             7413..9947
                     /gene="mrcB"
                     /locus_tag="ACHK49_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO9260986.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10167..12410
                     /gene="fhuA"
                     /locus_tag="ACHK49_000287"
     CDS             10167..12410
                     /gene="fhuA"
                     /locus_tag="ACHK49_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO9260987.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLSGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12461..13258
                     /gene="fhuC"
                     /locus_tag="ACHK49_000288"
     CDS             12461..13258
                     /gene="fhuC"
                     /locus_tag="ACHK49_000288"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO9260988.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13258..14148
                     /gene="fhuD"
                     /locus_tag="ACHK49_000289"
     CDS             13258..14148
                     /gene="fhuD"
                     /locus_tag="ACHK49_000289"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO9260989.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14145..16127
                     /gene="fhuB"
                     /locus_tag="ACHK49_000290"
     CDS             14145..16127
                     /gene="fhuB"
                     /locus_tag="ACHK49_000290"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO9260990.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVHAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16162..17442)
                     /gene="hemL"
                     /locus_tag="ACHK49_000291"
     CDS             complement(16162..17442)
                     /gene="hemL"
                     /locus_tag="ACHK49_000291"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO9260991.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17608..17655
                     /gene="yadX"
                     /locus_tag="ACHK49_000292"
     CDS             17608..17655
                     /gene="yadX"
                     /locus_tag="ACHK49_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO9260992.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17667..19088
                     /gene="clcA"
                     /locus_tag="ACHK49_000293"
     CDS             17667..19088
                     /gene="clcA"
                     /locus_tag="ACHK49_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO9260993.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDGARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAMQLARSKAASASENT"
     gene            19112..19177
                     /gene="yadW"
                     /locus_tag="ACHK49_000294"
     CDS             19112..19177
                     /gene="yadW"
                     /locus_tag="ACHK49_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO9260994.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19170..19514
                     /gene="erpA"
                     /locus_tag="ACHK49_000295"
     CDS             19170..19514
                     /gene="erpA"
                     /locus_tag="ACHK49_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO9260995.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19561..20184)
                     /gene="yadS"
                     /locus_tag="ACHK49_000296"
     CDS             complement(19561..20184)
                     /gene="yadS"
                     /locus_tag="ACHK49_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO9260996.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20222..21022)
                     /gene="btuF"
                     /locus_tag="ACHK49_000297"
     CDS             complement(20222..21022)
                     /gene="btuF"
                     /locus_tag="ACHK49_000297"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO9260997.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21015..21713)
                     /gene="mtnN"
                     /locus_tag="ACHK49_000298"
     CDS             complement(21015..21713)
                     /gene="mtnN"
                     /locus_tag="ACHK49_000298"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO9260998.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIEKRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21797..23314
                     /gene="dgt"
                     /locus_tag="ACHK49_000299"
     CDS             21797..23314
                     /gene="dgt"
                     /locus_tag="ACHK49_000299"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO9260999.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23444..24868
                     /gene="degP"
                     /locus_tag="ACHK49_000300"
     CDS             23444..24868
                     /gene="degP"
                     /locus_tag="ACHK49_000300"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO9261000.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLGDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            25025..26176
                     /gene="cdaR"
                     /locus_tag="ACHK49_000301"
                     /pseudo
     CDS             25025..26176
                     /gene="cdaR"
                     /locus_tag="ACHK49_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26265..26651)
                     /gene="yaeH"
                     /locus_tag="ACHK49_000302"
     CDS             complement(26265..26651)
                     /gene="yaeH"
                     /locus_tag="ACHK49_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO9261001.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26813..27625)
                     /gene="yaeI"
                     /locus_tag="ACHK49_000303"
     CDS             complement(26813..27625)
                     /gene="yaeI"
                     /locus_tag="ACHK49_000303"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFO9261002.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSAGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLVLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27680..28504)
                     /gene="dapD"
                     /locus_tag="ACHK49_000304"
     CDS             complement(27680..28504)
                     /gene="dapD"
                     /locus_tag="ACHK49_000304"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO9261003.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28535..31207)
                     /gene="glnD"
                     /locus_tag="ACHK49_000305"
     CDS             complement(28535..31207)
                     /gene="glnD"
                     /locus_tag="ACHK49_000305"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO9261004.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31269..32063)
                     /gene="map"
                     /locus_tag="ACHK49_000306"
     CDS             complement(31269..32063)
                     /gene="map"
                     /locus_tag="ACHK49_000306"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO9261005.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHTVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32431..33156
                     /gene="rpsB"
                     /locus_tag="ACHK49_000307"
     CDS             32431..33156
                     /gene="rpsB"
                     /locus_tag="ACHK49_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO9261006.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33414..34265
                     /gene="tsf"
                     /locus_tag="ACHK49_000308"
     CDS             33414..34265
                     /gene="tsf"
                     /locus_tag="ACHK49_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO9261007.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34412..35137
                     /gene="pyrH"
                     /locus_tag="ACHK49_000309"
     CDS             34412..35137
                     /gene="pyrH"
                     /locus_tag="ACHK49_000309"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO9261008.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35429..35986
                     /gene="frr"
                     /locus_tag="ACHK49_000310"
     CDS             35429..35986
                     /gene="frr"
                     /locus_tag="ACHK49_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO9261009.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36078..37274
                     /gene="ispC"
                     /locus_tag="ACHK49_000311"
     CDS             36078..37274
                     /gene="ispC"
                     /locus_tag="ACHK49_000311"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO9261010.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37463..38221
                     /gene="ispU"
                     /locus_tag="ACHK49_000312"
                     /gene_synonym="uppS"
     CDS             37463..38221
                     /gene="ispU"
                     /locus_tag="ACHK49_000312"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO9261011.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNSGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38234..39091
                     /gene="cdsA"
                     /locus_tag="ACHK49_000313"
     CDS             38234..39091
                     /gene="cdsA"
                     /locus_tag="ACHK49_000313"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO9261012.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39103..40455
                     /gene="rseP"
                     /locus_tag="ACHK49_000314"
     CDS             39103..40455
                     /gene="rseP"
                     /locus_tag="ACHK49_000314"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO9261013.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40485..42917
                     /gene="bamA"
                     /locus_tag="ACHK49_000315"
     CDS             40485..42917
                     /gene="bamA"
                     /locus_tag="ACHK49_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO9261014.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43039..43524
                     /gene="skp"
                     /locus_tag="ACHK49_000316"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43039..43524
                     /gene="skp"
                     /locus_tag="ACHK49_000316"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO9261015.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43528..44553
                     /gene="lpxD"
                     /locus_tag="ACHK49_000317"
     CDS             43528..44553
                     /gene="lpxD"
                     /locus_tag="ACHK49_000317"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO9261016.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44658..45113
                     /gene="fabZ"
                     /locus_tag="ACHK49_000318"
     CDS             44658..45113
                     /gene="fabZ"
                     /locus_tag="ACHK49_000318"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO9261017.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45117..45905
                     /gene="lpxA"
                     /locus_tag="ACHK49_000319"
     CDS             45117..45905
                     /gene="lpxA"
                     /locus_tag="ACHK49_000319"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO9261018.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45905..47053
                     /gene="lpxB"
                     /locus_tag="ACHK49_000320"
     CDS             45905..47053
                     /gene="lpxB"
                     /locus_tag="ACHK49_000320"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO9261019.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47050..47646
                     /gene="rnhB"
                     /locus_tag="ACHK49_000321"
     CDS             47050..47646
                     /gene="rnhB"
                     /locus_tag="ACHK49_000321"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO9261020.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47683..51165
                     /gene="dnaE"
                     /locus_tag="ACHK49_000322"
     CDS             47683..51165
                     /gene="dnaE"
                     /locus_tag="ACHK49_000322"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO9261021.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNRALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51178..52137
                     /gene="accA"
                     /locus_tag="ACHK49_000323"
     CDS             51178..52137
                     /gene="accA"
                     /locus_tag="ACHK49_000323"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO9261022.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52236..54377
                     /gene="ldcC"
                     /locus_tag="ACHK49_000324"
     CDS             52236..54377
                     /gene="ldcC"
                     /locus_tag="ACHK49_000324"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO9261023.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVALGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54434..54823
                     /gene="yaeR"
                     /locus_tag="ACHK49_000325"
     CDS             54434..54823
                     /gene="yaeR"
                     /locus_tag="ACHK49_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO9261024.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54888..56186
                     /gene="tilS"
                     /locus_tag="ACHK49_000326"
     CDS             54888..56186
                     /gene="tilS"
                     /locus_tag="ACHK49_000326"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO9261025.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56235..56495)
                     /gene="rof"
                     /locus_tag="ACHK49_000327"
     CDS             complement(56235..56495)
                     /gene="rof"
                     /locus_tag="ACHK49_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO9261026.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56482..56682)
                     /gene="yaeP"
                     /locus_tag="ACHK49_000328"
     CDS             complement(56482..56682)
                     /gene="yaeP"
                     /locus_tag="ACHK49_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO9261027.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56848..57393
                     /gene="yaeQ"
                     /locus_tag="ACHK49_000329"
     CDS             56848..57393
                     /gene="yaeQ"
                     /locus_tag="ACHK49_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO9261028.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57390..57812
                     /gene="arfB"
                     /locus_tag="ACHK49_000330"
     CDS             57390..57812
                     /gene="arfB"
                     /locus_tag="ACHK49_000330"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO9261029.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57826..58536
                     /gene="nlpE"
                     /locus_tag="ACHK49_000331"
                     /gene_synonym="cutF"
     CDS             57826..58536
                     /gene="nlpE"
                     /locus_tag="ACHK49_000331"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO9261030.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
BASE COUNT        13743 a        14544 c        16309 g        13967 t
ORIGIN      
        1 taccatttgc ttaatttgtc aaatgctctt gtatgtaaga cttcacgcaa cgtctcccca
       61 tcaggaaacg ccagctccgg tgcaatctca aacagcggcc acagcataaa tccacgattc
      121 ttcatatcgt agtgcggaac ggtcaggcgt tcggtattta tcacttcatt accaaacagc
      181 atgatatcga gatccagcgt gcgtggtccc cagcgttcag ctttacgaac acgaccctgt
      241 tgcagttcta tgcgctgtgt atggttgagt aactcttccg gggcgagcga agtttcaagc
      301 gccacggcgg cgttgaggta atcgggttga tcttgcggcc ccagcggtgg ggtgcggtaa
      361 aacgaagaaa cggcaagaat gtggctttca gggatatcgc ctaatgcttt cagggcagca
      421 ttgacctgct ccagcggaga ggccagattg ctgcctatgg caatatacgc cactgtcatg
      481 cggtaccctc acgacgcggt gcgcgtttgc gtggacgacg agtacggcga cgcggtgacg
      541 gctcttcatc caactcgtta agcatccctt tttgatctgg tggcgcggaa acctggaact
      601 caccccacca tttcaccaga cgctgcagtt cagcgttacg ttcaacttca gctcgcaagg
      661 ccaacaggtc ataagccgca cggaacttag gatgctccag cagtttccat gcgcgtttac
      721 cctgacgacg ggacatacgc aactgcaact gccagatatc gcgggttaac gtcgtcagac
      781 gtttcgggat tgccagtgaa cggcaggctt cgtccagcac gtcgttcatc gccagcgcga
      841 aagcgtcgtg ataggtcagg ccgctttcct gggcaatctt ctgtgccgtc tccagcagtg
      901 ggtaccagaa catggcggca aacaggaacg ccgggttcac gcgcatatcg ttatggatac
      961 gcgtatcggt attcttcagc acctgttcaa tgatccgctc catcgggctg tcgccatttt
     1021 ccgtgaagta gcgggtaatg gtcgggaaca gcggctggaa cagatgatat tcacacaaca
     1081 gcttataggt atcgtaaccg tagcccgctt gtagcagttt aagcgattct tcaaacaggc
     1141 gtgccggtgg gatatcgttc aacagggtag cgaggcgagg gatcggttct gcggtttccg
     1201 ggctgatgcg catacccaat ttggcggcaa aacgtaccgc gcgcagcata cgtaccggat
     1261 cttcacggta gcgcgtttcc gggttaccaa tcagacggat aacgccgtcc ttcagatcct
     1321 tcataccgcc aacgtaatca cggacggtaa aatccgctac gctgtaatag aggctgttga
     1381 tagtgaaatc gcggcgctgg gcgtcttctt cgatggagcc gaaaatgttg tcgcgcagca
     1441 acatgccgtt ttgcccgcgt tgggaggtcg tgcggtcgct gacgttacct tcgtggtgtc
     1501 cacggaaggt cgcaacttcg ataatctccg ggccaaacat tacatgagcc agacggaaac
     1561 ggcgacccac caggcggcag ttacggaaca gtttgcgcac ctgctcaggc gtggcgttag
     1621 tggttacgtc aaaatctttc ggctttttgc caagtaacag gtcgcgcacg ccgccgccaa
     1681 ccagccaggc ttcgtatccc gctttgttga gcctgtacat taccttcagg gcattttcac
     1741 tgatatcttt gcgggaaata gcatgctgct cacgcgggat caccgtcacc tgtggacggg
     1801 cgactgcctg ttcagcctcg ctttcctcgc ggcttagcac cttgcggcaa aaattagcga
     1861 ctcgggtaaa aatagtacac ctcggtagtg tcaaacatca ttcaggacaa aaaaatagcg
     1921 gctaatcata gctcagcatg acgcatttga gaatgttgaa tttacaattg ccgactcggg
     1981 cacggcggta agccgccagt ttttgacggc tgactgaagg atttgctcga cgctgaaatc
     2041 ctgccagtgt gcttctgcct gctgccccag aaattgaagt gccgcgatta gtaccgggcg
     2101 tggatcgcct ttcggcaacg caggcgcatg attctgcttg gaaagtttag cgccttgtgg
     2161 attaagcgcc agcggcagat gaatgtaatc tggcactttc cagccaaaaa gctggtacag
     2221 cgagatttgc cttactgttg gttcaatcag atcagcccca cgcactattt ctgtaacgcc
     2281 ctggaaatga tcatcaacca caacagccag gttgtaggcg aacaacccat cacggcgatg
     2341 aatgataaaa tcttcccgtg ccagtttttc gtcggcgtga ataatgccgc gcagctggtc
     2401 agtaaattgc gtgaccggat gctgctggcg gatacgcact gcggcattgt ctggtccatg
     2461 atgcaacacc cggcaatgac cgtcgtaaat accgccaatg ctttgaatac gcgcacgcgt
     2521 acaggtgcag taataactta gtccttgttc atgtaaccag gcgagtgctt cacgataggc
     2581 gtggtgacgt tgcgattgcc agagaacatc gccgtcccag tgcagaccgt aatgttccag
     2641 ctggcgcagg atagtttctg cggcaccggg aacttcacga ggcgggtcga tatcttctat
     2701 gcgtaccagc cagcgacctt gccgggcgcg agcctgcaaa tagctgccga gcgcggcgat
     2761 cagagagcca aaatgaagct cgccggaagg agagggggcg aagcggccaa tatactgtgt
     2821 gtctgtcatc tctttgaaca aaaaataagg cgggagcatt tcccgcctgt ggtaaacgtg
     2881 atggaacggc tgtaattagc cagccatctg tttttcgcga atttcagcca gcgttttgca
     2941 gtcgatgcac agatcggctg tcgggcgcgc ttccagacgg cgaataccaa tttcaacacc
     3001 gcaggattcg cagtagccga aatcttcgtc ttccactttt ttcagcgtct tctcgatctt
     3061 tttgatcagc ttacgctcgc gatcgcggtt acgcagttcg aggctgaact cttcttcctg
     3121 ggctgcacgg tctaccgggt ccgggaagtt ggctgcttca tcctgcatat gtgtaacggt
     3181 gcgatcgact tcatccctga gttgattacg ccatgcttcc agaatacgac ggaagtgcgc
     3241 cagctgggct tcattcatat actcttcgcc cggcttctcc tgatatggtt ccaccccagc
     3301 gatggcgaga atactcaggg acgatgtttt acggttttgc ccttcttgca tgttgcttct
     3361 ccttaacacg cactatcgat ccccatgttc gggggaaaaa tgaggccgct ataaatagca
     3421 gatgcttttc cggatagcaa ttatctaaac gtaacacttg acaactgtgt gaggaaaagc
     3481 gtatttgcgc acgcgaccag aatgtaaatt aaccagttac ttactttact acaatgtaac
     3541 cggcagtgat tttttaagag ccatgccttc agcagaaatt tccgctttgt aagccagaat
     3601 ttctaccccc ctctgttgag cttctgacaa tagttgcgcg tatttctcat cgatgtggcg
     3661 cgcgggtgaa aaccgtgtaa tggctgaatg cagcacggcg aaaaagataa ccgcacgctg
     3721 gccttcagcc gctacgctca tcaactcccg aaggtgtttc tgacctcgtt cagtgaccgc
     3781 atcgggaaaa tatccctgtt cgttctccgc taacgtaacc gatttcactt caatatagca
     3841 gtctggacgc gaatccgcct gcaacataaa gtcaatacgg ctgcgttctg cgccgtattt
     3901 tacttcgctt ttcagcgagc tatagcctga cagttctgaa attgattcat taaggatagc
     3961 ctctttcgtc aacctgttag cccaaagcgt gttgacgcaa ataaatgcgc cgctctggct
     4021 ttgagttaat tcccaggtgt gtgggtattt ccgtttggtg ttgtctgaag tcgaatacca
     4081 gacggtatcg ccaggcgttg cacaaccggt cattgcaccc gtattcgggc agtgtagcgt
     4141 taattcgcga ccatcgggtg tgatcacatc ggctaaaaaa cgtttgtaac gctgaattag
     4201 cgtcgcgcgc tgtagagggg gagaaaattc cattgcgaca atccttgtta ttgcgttagc
     4261 gcccagcgtt ttagcggcgt gtagcgtgtg cgtccacggg caaacgagga ggcgtaaagg
     4321 gtgaactccg tcaccgcata cgaccagtta aaacctggcg gcgggattgt caccgcctcg
     4381 ctggcgtcgc gcaataaggt aatatgtgga tgaaacggac gattgctttg aaaacaaccg
     4441 ctgcgggcag cctgtgaacg gagcatattc gccagctgga ttaagccgcg tggcggctga
     4501 cgcatcccta accacaccac acgcgaacgc agccattgtc cggcgtcatc aagcgtgagt
     4561 gtgaaaccag gttgacgaat ccgtccggct aaaagagaaa gcgccttctc tttctcggcg
     4621 ctcacttcgc ctaaaaatgc cagagtcaga tgcaaattat cggcggcgac cggacgtccc
     4681 gcctcaggtg ggaagtgtgt ggcgcgccag tggataatct gttcgcggat ttctgcaggt
     4741 aagtcgatag caaagaacag acgttgcggt tcagacatgt gaggcactcg gttatgaatt
     4801 accgcgatgc tacaatgtgg cgcgaagaat gttaaccctc tggagcgttt tgtgtcgtcg
     4861 ttgcccgttg ctgccgtctt acctgaatta cttaccgctc tcgattgtgc gccgcaggta
     4921 ttattaagtg cgccgaccgg ggccgggaaa tcaacctggc tgccgctgca actgctggcg
     4981 catcccggca ttaacgggaa aattatcctg ctggagccgc gtcgtctggc ggcgcgtaac
     5041 gtcgcgcaac ggctggcgga gctgcttaac gaaaagccag gcgataccgt tggctaccgg
     5101 atgcgtgcgc aaaactgcgt cgggccgaat acccgcctgg aagtagttac cgaaggcgtg
     5161 ctgacgcgga tgatccagcg tgacccggag ttgagcggtg ttggattggt gatcctcgat
     5221 gagtttcatg agcgcagctt gcaggcggat ttggcgttgg cgctgttact cgatgtgcaa
     5281 caaggtctgc gtgatgacct taaactgctg attatgtcgg ctacgctgga caacgaccgc
     5341 ttgcagcaaa tgctgccaga agcgcccgtc gtcatctcag aagggcgctc gtttccggtt
     5401 gaacgccgtt atttaccact gcccacgcat cagcgttttg acgaagccgt tgcggtagcc
     5461 accgctgaaa tgctgcgtca ggaaagtgga tcattactgt tatttttacc tggcgtcgga
     5521 gaaattcagc gtgtgcagga acaactggct tcgcgcatcg gcagcgatgt attgctctgc
     5581 ccgctgtatg gcgcgttgtc gctgaacgat cagcgaaaag cgatcctccc ggcaccgcaa
     5641 gggatgcgca aagtggtgct ggcgaccaat attgctgaaa ccagtttaac catcgaaggt
     5701 attcgtctgg tggtggattg tgcccaggag cgtgtggcgc gttttgatcc gcgcacgggg
     5761 cttacgcgac tgattactca acgcgttagc caggcatcca tgacgcagcg tgccgggcgc
     5821 gccgggcgtc tggagccggg tatctgcctg catttaatcg ccaaggaaca agcagaacgc
     5881 gccgcggcgc aaagtgaacc ggagatatta caaagcgatc tttccggttt gctgatggaa
     5941 ttactgcaat ggggatgcag cgatccggcg cagatgagct ggctggatca accgccagta
     6001 gtgaatctac tggccgcgaa acgtctgtta caaatgctgg gggcactgga gggtgaacgg
     6061 cttagtgcgc aagggcaaaa aatggcagcg ctgggtaacg atccgcgttt agcggcaatg
     6121 ctggttagcg cgaagaacga cgacgaagct gctaccgcgg caaaaattgc cgccattctc
     6181 gaagagccgc cacggatggg caatagtgac ctgggcgtgg cgttttcgcg caatcaacca
     6241 gcctggcagc aacgtagtca gcaactgtta aaacgcttaa acgtacgtgg cggtgaggca
     6301 gacagttcgc ttatcgcgcc gctacttgcc ggggcgtttg ccgatcgcat tgctcgtcgc
     6361 cgtgggcaag atggacgcta tcaactggca aacggcatgg gagcgatgct cgatgccaac
     6421 gacgcgctaa gccgccacga atggttgatc gcaccgttat tattgcaggg cagcgcctcg
     6481 ccggatgcgc ggattttact ggcgctgccg gtcgatattg atgagttagt acaacgctgc
     6541 ccgcagctgg tacagcagtc tgacactgtg gagtgggatg acgcgcaagg tacgctgaaa
     6601 gcctggcgtc ggctgcaaat cggtctgctg acggtgaaag tgcagccgct ggcgaaaccc
     6661 tcggaagacg agttgcatca ggcgatgctt aatggcatcc gcgataaaga tttaagcgtg
     6721 ctcaactgga cggcggaagc ggaacagcta cgcttgcgtt tgttatgcgc cgcaaagtgg
     6781 ttgccggaat atgactggcc agcggttgat gatgaaagtt tattggcgac gctggaaacg
     6841 tggctgctgc cacatatgac tggcgtacat tcactacgcg gcctgaaatc actcgacatt
     6901 tatcaggcac tacgcggatt acttgattgg ggaatgcagc aacgtctgga tagtgaattg
     6961 cctgcgcatt acactgtgcc gacgggaagc cggatcgcca ttcgttatca tgaagataac
     7021 ccgcccgcgc tggcggtgag aatgcaagag atgtttggcg aggccaccaa tccgacgatc
     7081 gcccaggggc gcgtgccgct ggtgctggag ttgctttcac ctgcccaaag gccattacaa
     7141 atcacacgag atttgagcgc cttctggaaa ggagcgtacc gtgaggtgca aaaagagatg
     7201 aaagggcgtt atcccaaaca tgtctggccg gacgacccgg caaatactgc accgacgcga
     7261 cggacgaaaa agtattcgta aggttggttt tctccctctc cctgtgggag aggggaccga
     7321 tcgagcacaa attttgagag atatcttctt ctgtcttgta acagaagaac agaaaatcgg
     7381 gcttttgcgc ctgaatattg cggagaaaaa gcatggccgg gaatgaccgc gagccaattg
     7441 gacgcaaagg gaaaccgacg cgtccggtca aacaaaaggt aagccgtcgt cgttacgaag
     7501 atgacgatga ttacgacgat tatgatgact atgaggatga agaaccgatg ccgcgcaaag
     7561 gtaagggcaa aggcaaaggg cgtaagcctc gtggcaaacg cggctggcta tggctgctgt
     7621 taaaactggc tatcgttttt gccgtgctga tcgccattta cggcgtttat ctcgatcaaa
     7681 aaattcgtag ccgtattgat ggcaaggtct ggcaactgcc tgcggcagtt tatggccgaa
     7741 tggtcaacct tgagccagat atgaccatca gcaagaacga gatggtgaag ctgctggagg
     7801 cgactcagta tcgtcaggtg tcgaaaatga cccgtcctgg cgaatttacc gtgcaggcca
     7861 acagcattga gatgatccgc cgtccgtttg atttcccgga cagtaaagaa gggcaggtgc
     7921 gtgcgcgtct gacctttgat ggcgatcatc tggcgacgat cgtcaatatg gagaacaacc
     7981 gtcagttcgg tttcttccgt cttgatccgc gtctgatcac catgatctcc tcgccaaacg
     8041 gtgagcagcg tttgtttgtg ccgcgcagtg gtttccctga tttgctggtg gatactttgc
     8101 tggcgacaga agaccgtcat ttttacgagc atgacggcat cagcctctac tccatcggac
     8161 gtgcggtgct ggcaaacctg accgccggac gcacggtgca gggagcgagt acgctgacgc
     8221 aacagctggt gaaaaacctg ttcctctcca gtgagcgttc ttactggcgt aaagcgaacg
     8281 aagcttacat ggcgctgatc atggacgcgc gttacagcaa agaccgtatt cttgagctgt
     8341 atatgaacga ggtgtatctc ggtcagagcg gcgacaacga aatccgcggc ttcccgctgg
     8401 cgagcttgta ttactttggt cgcccggtag aagagctaag cctcgaccag caggcgctgt
     8461 tagtcggcat ggtgaaagga gcgtccatct acaacccgtg gcgtaaccca aaactggcgc
     8521 tggagcgacg taatctggtg ctgcgtctgc tgcaacagca acagattatt gatcaagaac
     8581 tctatgacat gttgagtgca cgtccgctgg gtgttcagcc acgcggtggg gtgatctctc
     8641 cacagccagc ctttatgcaa ctggtgcgtc aggagctgca ggcgaaatta ggcgataagg
     8701 taaaagatct ctccggcgtg aagatcttca ctacctttga ctcggtggcc caggacgcgg
     8761 cagaaaaagc cgccgtggaa ggcattccgg cactgaagaa acagcgtaag ttgagcgatc
     8821 ttgaaactgc gattgtggtc gtcgaccgct ttagtggtga agttcgtgcg atggtcggtg
     8881 gttctgagcc gcagtttgcg ggctacaacc gtgcgatgca ggcgcgtcgt tcgattggtt
     8941 cccttgcaaa accagcgact tatctgactg ccttaagcca gccgaaaatc tatcgtctga
     9001 atacgtggat tgcggatgcg ccaatcgcgc tgcgtcagcc gaatggccag gtctggtcac
     9061 cgcagaatga tgaccgtcgt tatagcgaaa gcggcagagt gatgctggtg gatgcgttga
     9121 cccgttcgat gaacgtgccg acggtaaatc tggggatggc gctggggctg cctgcggtta
     9181 cggagacctg gatcaaactg ggcgtaccga aagatcagtt gcatccggtt ccggcgatgc
     9241 tgctgggggc gttgaactta acgccaatcg aagtggcgca ggcgttccag accatcgcca
     9301 gcggcggtaa ccgtgcaccg ctttctgcac tgcgttcggt aattgcggaa gatggcaagg
     9361 tgctgtatca gagcttcccg caggcggaac gcgctgttcc ggcgcaggcg gcgtatctga
     9421 cactatggac catgcagcag gtggtacaac gcggtacggg tcgtcagctt ggggcgaaat
     9481 acccgaacct gcatctggca gggaaaacag ggactaccaa caataacgta gatacctggt
     9541 ttgcaggcat tgacggcagc acggtgacca tcacctgggt aggccgtgat aacaaccagc
     9601 cgaccaaact gtatggagcc agcggggcaa tgtcgattta tcagcgttat ctggctaacc
     9661 agacgccaac gccgctgaat cttgttccgc cagaagatat tgcagatatg ggcgtggact
     9721 acgacggcaa ctttgtttgc agcggtggca tgcgtgtctt gccggtctgg accagcgatc
     9781 cgcaatcgct gtgccagcag agcgagatgc agcagcagcc gtcaggcaat ccgtttgatc
     9841 agtcttctca gccgcagcaa cagccgcaac agcaacctgc tcagcaagag cagaaagaca
     9901 gcgacggtgt agccggttgg atcaaggata tgtttggtag taattaacat ctaagcgtga
     9961 aataccggat ggcgagttgc catccggtaa aataacatcc catctaagat attaaccctt
    10021 tcttttcatc tggttgttta ttaacccttc aggaacgctc agattgcgta ccgcttgcga
    10081 acccgccagc gtttcgaata ttatcttatc tttataataa tcattctcgt ttacgttatc
    10141 attcacttta catcagagat ataccaatgg cgcgttccaa aactgctcag ccaaaacact
    10201 cactgcgtaa aatcgcagtt gtagtagcca cagcggtaag cggcatgtct gtttatgcac
    10261 aggcagcggt tgaaccgaaa gaagacacta tcaccgttac cgctgcacct gcgccgcaag
    10321 aaagcgcatg ggggcctgct gcaactattg cggcgcgaca gtctgctacc ggcactaaaa
    10381 ccgatacgcc gattcaaaaa gtgccacagt ctatttctgt tgtgaccgcc gaagagatgg
    10441 cgctgcatca gccgaagtcg gtaaaagaag cgcttagcta cacgccgggt gtctctgttg
    10501 gtacgcgtgg cgcatccaac acctatgacc acctgatcat tcgcggcttt gcggcagaag
    10561 gccaaagcca gaataactat ctgaatggcc tgaagttgca gggcaacttc tataacgatg
    10621 cggtcattga cccgtatatg ctggaacgcg ctgaaattat gcgtggcccg gtttccgtgc
    10681 tttacggtaa aagcagtcct ggcggcctgt tgaatatggt cagcaagcgt ccgaccaccg
    10741 aaccgctgaa agaagttcag tttaaagccg gtactgacag cctgttccag actggttttg
    10801 actttagcga ttcgttggat gatgacggtg tttactctta tcgcctgacc ggtcttgcgc
    10861 gttctgccaa tgcccagcag aaagggtcag aagagcagcg ttatgctatt gcaccggcgt
    10921 tcacctggcg tccggatgat aaaaccaatt tcaccttcct ttcttacttc cagaacgagc
    10981 cggaaaccgg ttattacggc tggttgccga aagagggaac cgttgagccg ctgccgaacg
    11041 gtaagcgtct gccgacagac tttaatgaag gggcgaagaa caacacctat tctcgtaatg
    11101 agaagatggt cggctacagc ttcgatcacg aatttaacga cacctttact gtgcgtcaga
    11161 acctgcgctt tgctgaaaac aaaacctcgc aaaacagcgt ttatggttac ggtgtctgct
    11221 ccgatccggc gaatgcttac agcaaacagt gtgcggcatt agcgccagcg gataaaggcc
    11281 attatctggc acgtaaatac gtcgttgatg atgagaagct gcaaaacttc tccgttgata
    11341 cccagttgca gagcaagttt gccactggcg atatcgacca caccctgctg tccggtgtcg
    11401 actttatgcg tatgcgtaat gacatcaacg cctggtttgg ttacgacgac tctgtgccac
    11461 tgctcaatct gtacaatccg gtgaataccg atttcgactt caatgccaaa gatccggcaa
    11521 actccggccc ttaccgcatt ctgaataaac agaaacaaac gggcgtttat gttcaggatc
    11581 aggcgcagtg ggataaagtg ctggtcaccc taggcggtcg ttatgactgg gcagatcaag
    11641 aatctcttaa ccgcgttgct gggacgaccg ataaacgtga tgacaaacag tttacctggc
    11701 gtggtggtgt taactacctg tttgataatg gtgtaacacc ttacttcagc tatagcgaat
    11761 cgtttgaacc ttcttcgcaa gttgggaagg atggtaatat tttcgcaccg tctaaaggta
    11821 agcagtatga agtcggcgtg aaatatgtac cggaagatcg tccgattgta gttactggtg
    11881 ccgtgtataa tctcactaaa accaacaacc tgatggcgga ccctgagggt tccttcttct
    11941 cggttgaagg tggcgagatc cgcgcacgtg gcgtagaaat cgaagcgaaa gcggcgctgt
    12001 cggcgagtgt taacgtagtc ggttcttata cttacaccga tgcggaatac accaccgata
    12061 ctacctataa aggcaatacg cctgcacagg tgccaaaaca catggcttcg ctgtgggctg
    12121 actatacctt ctttgacggt ccgctttcag gtctgacgct gggcaccggt ggtcgttata
    12181 ctggctccag ctatggtgat ccggctaact cctttaaagt gggaagttat acggtcgtgg
    12241 atgcgttagt gcgttatgat ctggcgcgag tcggcatggc gggctccaac gtggcgctgc
    12301 atgttaacaa cctgttcgat cgtgaatacg tcgccagctg ctttaacact tatggctgct
    12361 tctggggcgc agaacgtcag gtcgttgcaa ccgcaacctt ccgtttctaa tttctctttt
    12421 ggggcacgga tttccgtgcc catttcacaa gttggctgtt atgcaggaat acacgaatca
    12481 ttccgatacc acttttgcac tgcgtaatat ctcctttcgt gtgcccgggc gcacgctttt
    12541 gcatccgctg tcgttaacct ttcctgccgg gaaagtgacc ggtctgattg gtcacaacgg
    12601 ttctggtaaa tccactctgc tcaaaatgct tggccgtcat cagcctccgt cagaagggga
    12661 gattcttctt gatgcccagc cgctggaaag ctggagcagc aaagcgtttg cccgcaaagt
    12721 ggcttatttg ccgcagcagc ttcctccggc agaagggatg accgtgcgtg aactggtggc
    12781 gattggtcgt tacccgtggc atggcgcgct ggggcgcttt ggtgcggcag atcgcgaaaa
    12841 ggtcgaggaa gctatctcgc tggttggctt aaaaccgctg gcgcatcggc tggtcgatag
    12901 tctctctggc ggcgaacgtc agcgggcgtg gatcgccatg ctggtggcgc aggatagccg
    12961 ttgtctgttg ctcgacgaac cgacttcggc gctggatatc gcccaccagg ttgatgtgct
    13021 ggcgctggtg caccgtttaa gtcaggaacg tggcctgacg gtcattgccg tgctgcacga
    13081 tatcaatatg gcggcacgct actgtgatta tctggtcgcc ctgcgcggtg gtgaaatgat
    13141 tgctcaggga acgcctgcgg aaattatgcg cggcgaaacc ctcgaaatga tttatggcat
    13201 cccgatgggt attttgccgc atccggcggg tgctgcacct gtgagttttg tttattgatg
    13261 agcggcttac ctcttatttc gcgccgtcga ctgttaacgg cgatggcgct ttctccgttg
    13321 ttatggcaga tgaataccgc ccacgcggcg gctattgacc ccaatcgtat tgtggcgctg
    13381 gagtggttgc cggtggaatt actgctggcg ctcggcatcg tgccttacgg cgtggcggat
    13441 accatcaact atcgcctgtg ggtcagcgaa ccaccattgc cggactcagt gattgacgtc
    13501 ggtttgcgta cagaacctaa ccttgaactg ctgaccgaaa tgaaaccgtc gtttatggtc
    13561 tggtcggcag gatatggccc ttcaccagaa atgctggcac gtattgcgcc gggtcgcgga
    13621 tttaacttca gtgacggcaa acagccgttg gcgatggcgc gtaaatcgct gacggaaatg
    13681 gcagatttac ttaacctgca aagcgcagca gaaacgcatt tagcgcacta tgaagacttt
    13741 atccgcagca tgaaaccccg ctttgtgaag cgtggtgcac gcccgttact gctgacgacg
    13801 cttatcgatc cgcgccatat gctggtcttc ggtccaaaca gcttgttcca ggaaattctc
    13861 gatgagtacg gcatcccaaa tgcctggcaa ggggaaacca acttctgggg cagtaccgcc
    13921 gtcagtatcg atcgtctggc ggcgtataaa gacgttgatg tgctctgttt tgatcacgac
    13981 aacagcaaag acatggatgc gctaatggca accccgctgt ggcaggccat gccgtttgtc
    14041 cgtacagggc gctttcagcg cgtacccgca gtctggtttt atggtgcaac gctttcggca
    14101 atgcactttg tgcgtgttct ggataacgcc atcggaggta aagcgtgagt aaacgaattg
    14161 cgcttttccc ggcgttattg ctggcgctgt tagtgattgt cgctacggcg ctaacctgga
    14221 tgaacttctc acaggcgctg ccgcgcagtc agtgggcgca ggccgcctgg tcgccggata
    14281 ttgacgtcat cgagcagatg atttttcact acagcttgtt gccgcgtctg gcgatttcgc
    14341 tgctggtggg cgcaggcctg gggctggtgg gcgtgctgtt tcagcaagtg ctgcgtaatc
    14401 cgctggcgga accgacgaca cttggcgttg ctacaggcgc gcaactggga attaccgtca
    14461 ccacactttg ggcgatccca ggtgctatgg cgagccagtt tgccgcactg gtgggcgctt
    14521 gtgtcgttgg cttaatcgtc tttggcgtcg cgtgggggaa acggctttcg ccggttacgc
    14581 ttattctcgc ggggctggtg gtgagccttt attgcggggc aattaatcag ctactggtta
    14641 tcttccatca cgaccagctg caaagcatgt tcctgtggag caccggaacg ctgacgcaaa
    14701 ccgactgggg cggcgttgag cgtttatggc cgcagttgct gggcggcgtg atgttgacgt
    14761 tattgctact tcgcccgtta accctgatgg ggcttgatga tggcgtggcg cgcaatctcg
    14821 ggctggcctt gtcgctcgcg cgtctggcag cgctgtcgct ggcgattgtc atcagtgcgc
    14881 tgctggtgca cgctgtgggg attatcggct ttatcgggtt gttcgcgccg ctgctggcaa
    14941 aaatgctggg ggcgcggcgt ctgctgccac gactgatgct ggcgtcgctg attggggcgc
    15001 tgatcctctg gctttccgat caaatcatcc tctggctgac tcgcgtgtgg atggaagtgt
    15061 ccaccggttc ggtcactgcg ttgatcggtg cgccgctgct actgtggctg ttgccgcgtt
    15121 tacgcagcat tagcgcgccg gatatgaagg tcaacgatcg tgtcgcggct gaacgccaac
    15181 atgtgctggc gtttgccctc gcgggcggcg tgctgctgtt gatggctgtg gtggtggcgc
    15241 tgtcgtttgg tcgtgatgcg cacggctgga cgtgggcgag cggggcgttg cttgaggatt
    15301 tgatgccctg gcgctggccg cgaattatgg cggcgctgtt tgcgggcgtc atgctggcgg
    15361 tggcgggctg tattattcag cggctgaccg gaaacccgat ggcaagcccg gaagtgctgg
    15421 ggattagctc tggcgcagcg tttggcgtgg tgttgatgct gtttctggtg ccgggtaatg
    15481 cctttggctg gctgttacct gcaggcagtc tcggcgcggc ggtgacgctg ttgatcatta
    15541 tgatcgccgc cggccgcggt ggattttccc cacaccgcat gttactggcg gggatggcgt
    15601 taagcaccgc gttcaccatg cttttgatga tgttgcaggc aagtggtgac ccgcgaatgg
    15661 cgcaagtgct gacctggatt tccggttcga cctacaacgc gaccgatgcg caggtctggc
    15721 gcacgggaat tgtgatggtg attttgctgg cgattacccc gctgtgccgc cgctggctga
    15781 ccattttacc gctgggtggt gataccgccc gagccgtagg aatggcgctg acgccgacgc
    15841 gaattgcgct gctgctgtta gcggcttgcc tgacggcgac cgcgacgatg actattggac
    15901 cgttgagttt tgttggttta atggcaccgc atattgcgcg gatgatgggc tttcgacgga
    15961 cgatgccaca catcgtaatt tcggcgctgg tgggtggttt actgctggtg ttcgctgact
    16021 ggtgtgggcg gatggtgctg tttccattcc agatcccagc agggctgctg tcgaccttta
    16081 tcggcgcgcc atattttatc tatttgttga gaaagcagag ccgttaatag aatgcctcgc
    16141 ccggtgggcg aggcaaaatc atcacaactt cgcaaacacc cgtcgtgcag catcgatggt
    16201 gttattgata tcttccatgc tgtgcgccac ggacataaag cccgcttcaa acgctgacgg
    16261 tgccaggtaa acaccttcgt ccagcatcat atggaagaaa cgcttaaagc gttccacgtc
    16321 acaggccatc acatcctgat agcacgtcac ggactcggcg tcggtaaaga aaataccgaa
    16381 catgccgcca acgtggttaa cgaccagcgg aattccggct tcttctgccg cttccagcag
    16441 accttctgcc agacgtgttg tcagctcatc cagcgtttcg tgaacgcccg gctgcgcgac
    16501 ttcattcaga caggcgaaac ccgctgccat cgcaattggg ttaccggaaa gcgtacccgc
    16561 ctgatagacc ggacccgtcg gggccagcgc atccattaca tcacgacgac caccgaatgc
    16621 gcctaccggc attccaccgc cgatgatttt gcccaggcag gtgagatccg gttccacacc
    16681 gtaataatcc tgtgcgccag ctagcgctac gcggaagcca gtcattactt catcgatgat
    16741 cagcaatgcg ccaaattcgt cgcacagcgc gcgcagacct ggcaggaact ctggcagcgg
    16801 tggaacgcag ttcatattgc ctgccaccgg ctcgacgata atacaggcaa tctcttgcgg
    16861 gtattgctca aacgcggcgc gtacagaagc cagatcgtta taagtacagg ttaaggtatg
    16921 tttggcgaaa tctgccggaa cacccggcga gtttggctgg cctaacgtga gtgcgccaga
    16981 accggctttc accagcaggc agtcagcgtg accgtggtaa caaccttcaa atttaatgat
    17041 tttgtcgcga ccggtaaaac cacgggccag gcggatggcg ctcatcgtcg cttcagtgcc
    17101 ggagttcacc atgcgcacca tatccatggt cgggaccagt tcggtcacca gttgcgccat
    17161 tttcacttcc atttcggtcg gtgcaccaaa gcttaaacca cgctcggcgg cttcaatcac
    17221 ggcattgcgg atagccggat ggttatggcc cagcaccatc ggcccccagg aaccgacata
    17281 atcgatatag gctttgccat caacatcgta cagataagcg ccgtccgctt tttcgataaa
    17341 cagtggagtg ccgcccacgc cagtaaaggc gcgaacaggg gagttcacac cgccagggat
    17401 cagctcgcgc gctgcgctgt aaagattttc agacttactc atggagggtt cctgattcgt
    17461 agaaaaagtg aatggctgct attctatgtt attcataaca agttaaatac tcgtcaaaca
    17521 tcaggctgct tgtactggtg caatcctgat ttcttagagt ataaaagttt tgtgcatttg
    17581 aaacattacg ctttgcaaag gattttcatg gaacgtgcga gtaaaatgcc gtcatcttat
    17641 ttgtatgacc aataagtgat cattggatga aaactgatac tccctcttta gaaacaccgc
    17701 aggccgcgcg cctgcgacgc agacaactga ttcgccaact tcttgagcgc gataaaaccc
    17761 cgttagccat tttgtttatg gcggcagtcg tcggcacgct tgtcgggctg gcagcggttg
    17821 cttttgacaa aggtgtcgcc tggttgcaga accaacgtat gggggcgctg gtacatactg
    17881 ctgataatta tccgcttctg ttaaccgtcg cttttctctg ttcggcggtg ctggcgatgt
    17941 tcggctactt tttggtgcgc aaatacgcgc cggaagcagg tggttcgggg atcccggaaa
    18001 ttgaaggggc gctggaagat caacgtcccg ttcgctggtg gcgtgtgttg ccggtgaaat
    18061 tctttggcgg gctggggaca ctcggcggag gcatggtgct agggcgcgaa ggaccaaccg
    18121 tgcagatcgg cggcaatatc ggtcgcatgg tgcttgatat tttccgcctg aaaggtgatg
    18181 gagctcgcca tacgctgctg gcaaccggtg ctgctgcggg gctggctgcg gcctttaacg
    18241 cgccgctggc gggtattttg tttattatcg aagagatgcg tccgcagttt cgctatacgt
    18301 taatttcgat taaagcggta tttattggcg tcattatgtc gaccattatg taccggattt
    18361 ttaatcatga agttgcgttg attgacgtcg gtaaactttc tgatgcgccg cttaatacgc
    18421 tgtggcttta tctgatcctc ggtattattt ttggcatttt cggccctatt tttaataaat
    18481 gggtgctggg gatgcaggat ttgctgcacc gtgtgcacgg cggcaatatt accaaatggg
    18541 tactgatggg tggtgcgatt ggcggtctgt gtggattgct ggggtttgtg gcaccagcaa
    18601 cgtcgggcgg cggttttaac ctgattccta tcgctaccgc ggggaatttc agcatgggaa
    18661 tgctggtgtt tattttcgtc gctcgggtca ttaccacctt actctgcttc tcttccggcg
    18721 cgccgggcgg tatttttgcc ccgatgctgg cgctgggtac tgtgctggga accgctttcg
    18781 gaatggttgc cgttgagctg tttccgcaat atcacctgga ggcggggacg tttgctattg
    18841 ccggaatggg ggcattactg gcggcatcta ttcgcgcgcc gttaacgggg atcattctgg
    18901 ttctggagat gaccgataac taccagctca ttttgccaat gattattacc ggtcttggcg
    18961 caacactatt agcgcaattt accggcggga aaccgctata ctcggcgatt cttgcgcgca
    19021 cgctggcaaa acaggaagct atgcaactgg cgcgaagcaa ggccgcatca gccagcgaga
    19081 atacttgaac gaaataccag ggtattagat aatggcgatt attattgggt tagaatttgc
    19141 ccaattgccg atgtcgtttg gagcaaaata tgagtgatga cgtagcactg ccgctggagt
    19201 ttaccgacgc agcagccaac aaagttaaaa gcctgatcgc tgacgaagat aacccgaatc
    19261 tgaaattacg cgtgtatatc accggtggcg gttgcagcgg cttccagtat ggtttcacct
    19321 ttgatgatca ggtgaacgaa ggcgatatga ccatcgaaaa acagggcgtt ggcttggtgg
    19381 ttgatccgat gagcctgcaa tatctggtcg gcggttccgt tgactatacc gaaggactgg
    19441 aaggttctcg tttcatcgtg accaacccga acgcgaaaag cacctgcggt tgcggttctt
    19501 cctttagcat ctaatctgtt gttcatgtgc cggataaact atccggcaca tcttctctct
    19561 tcaacgccca ttctcatcca gcgcaaacgt cggtagctta agatgccaac gaatagccgc
    19621 cagccgaatc aagagcgtca cgaccatgcc catcatactg gctgtttcca gtggtacgga
    19681 aaatgtgtaa taagccgtag cgtggacaat accgccgata atacaggcag ttgcgtagat
    19741 ttctgtacgt aaaatcatgg ggatttcgcg ggccagaaca tcacgaatga tcccgccgcc
    19801 aacgccagta atgacgccca tacaaaccgc gattaacgga ccggcttccg cattaaaggc
    19861 tttattcacg ccaatgccga caaacaccgc cagaccaacg gcgtccagca ccggcaacat
    19921 ccattttggt aagcgtcttg gctggcgcac cagcacgatg gtcagcatgc tggttaccat
    19981 tgcaacgacc agatcggtgg gatctttcac ccaaaatacc gggccgtgat ccagcgccat
    20041 gtcgcgaatt gtcccgccgc ctacagcggt aaccacgccc agtaccagaa caccaaaagg
    20101 gtccatacgc aattttccgg ctaacaaaac gccggagatg gcaaataccg ctgtgccgac
    20161 tatatccagc caatagacga gcattgttca atccccactg agcacctgaa aaggtcaggc
    20221 actaatctac ctgtgaaagc gcattacaga gctgttgtgc agcgaggata atacgtgggc
    20281 ttgcacgttc aaaccagtca ctcgtgagag gaataacggg aattttgagc tgttcacccc
    20341 agtattgttt gattttagga atttggtccg gtccgcctgt aatgacaatc gcctgtggcg
    20401 agcgtgctaa cacctgttcg cggctaactt gcggccaggg aacccggctg tctttaaaga
    20461 tgttttctcc gccacaaact tcgagtacct ggttctgaat cgactctttt ccactggtaa
    20521 atggcggatt aatgccgaat tgcagaaaaa cacgtttttt aggtttatca gcatattgcg
    20581 ctttcaattg cgcgtactga tccagcaggg attgcgcggc ttgttcggcc ttgtctggtt
    20641 gcggactcca gggggccagt tgacgtaacg cattggcaat ttgttcaatg cttgtcgcat
    20701 cgacccacat cacttttatt cccagcgaag ccagctggtc aacctgccgc tcggcattac
    20761 ctccacgcca ggcaatcacc agatcgggtt tcagcgcgac aatgcgttcc agattcatcc
    20821 cctgccaggt ggaaacctgc tcaatctttt gcgcttgtgg aggatagtcg gaatagctgc
    20881 tgaccccaac cggcgtgatc ccggcggcaa aggcaagttc agtgttggcg ggagaaagcg
    20941 tgatgacgcg cggcgcggcg ttgagccaca gtggcgcaag aaaagacagg gcgaccagcg
    21001 ccctgaacag tgacttagcc atgtgcaagt ttctgcacca gtgactcaac catcaggctg
    21061 gactgtttag cggcaacagc caggaactca tcgaagctaa gatgagactg ttgatcggcc
    21121 acgtcggaga tggcgcgtac gacaacaaac gggacgttga aattgtggca gacatgggcg
    21181 attgccgtcg cttccatctc tacagcaatg gcctgtggga agttgtggcg gattttcgcc
    21241 agaccaacag aaccgttgat gaaagcgtcg ccgctaacaa tcaggccacg tacagcgtta
    21301 agattcagtt cggcaatgca ggcctcagcg gcagcgatca gtttatcgtc agctttaaag
    21361 cctgccggac agcctggtaa ctgaccgtat tcataaccaa atgccgtgac atccgcgtcg
    21421 tgataacgtg cttcgtccga gacaacgata tcgcccactt tcaacgttgg tgccaggcca
    21481 ccggcagaac cggtgttaat aatcacatct ggcttgcagt gttccaacag caaagtggca
    21541 cccagcgccg cagcgacttt accgatgccc gatttcagaa gcgcaacctc ggttccattc
    21601 agttggccgg tatagatttc gcaaccgccg agactgatag tttgacgttt ttcgattttg
    21661 tcacgcagca gcgtaacttc ttcttccatt gcaccaatga tgccgatttt catagattta
    21721 ctcgcgataa gcccgatttg aaggcatagt ttaccatgcg cttacgggga agcgtatttc
    21781 tcacgcggga gaggacatgg cacagattga tttccgaaaa aaaataaact ggcatcgtcg
    21841 ttaccgttca ccgcagggcg ttaaaaccga acatgagatc ctgcggatct tcgagagcga
    21901 tcgcgggcgt atcatcaact ctccggcaat tcgtcgtctg caacaaaaga cgcaggtttt
    21961 tccactggag cgcaatgccg ccgtgcgcac gcgtcttacc cactcgatgg aagtccagca
    22021 ggtggggcgc tacatcgcca aagaaatttt aagccgtctg aaagagctta aattgctgga
    22081 agcatacggc ctggatgaac tgaccggacc ttttgaaagc attgttgaga tgtcatgcct
    22141 gatgcacgat atcggcaatc cgccgtttgg tcattttggc gaagcagcga taaatgactg
    22201 gtttcgccaa cgtttgcacc cggaagatgc cgaaagccag cctctgactg acgatcgctg
    22261 cagcgtggcg gcactacgtt tacgggacgg ggaagaaccg cttaacgagc tgcggcgcaa
    22321 gattcgtcag gacttatgtc attttgaggg gaatgcacaa ggcattcgtc tggtgcatac
    22381 attgatgcgg atgaatctca cctgggcaca ggttggcggt attttaaaat atacccgtcc
    22441 ggcgtggtgg cgtggcgaaa cgcctgagac acatcactat ttaatgaaaa agccgggtta
    22501 ttatctttct gaagaagcct atattgcccg gttgcgtaaa gaacttaatt tggcgcttta
    22561 cagtcgtttt ccattaactt ggattatgga agctgccgac gacatctcct attgtgtggc
    22621 agaccttgaa gatgcggtag agaaaagaat atttaccgtt gagcagcttt atcatcattt
    22681 gcacgaagcg tggggccagc atgagaaagg ttcgctcttt tcgctggtgg ttgaaaatgc
    22741 ctgggaaaaa tcacgctcaa atagtttaag ccgcagtacg gaagatcagt tttttatgta
    22801 tttacgggta aacaccctaa ataaactggt accctacgcg gcacaacgat ttattgataa
    22861 tctgcctgcg attttcgccg gaacgtttaa tcatgcatta ttggaagatg ccagcgaatg
    22921 cagcgatctt cttaagctat ataaaaatgt cgctgtaaaa catgtgttta gccatccaga
    22981 tgtcgagcag cttgaattgc agggctatcg ggtcattagc ggattattag agatttatcg
    23041 tcctttatta agcctgtcgt tatcagactt tactgaactg gtagaaaaag aacgggtgaa
    23101 acgtttccct attgaatcgc gcttattcca caaactctcg acgcgccatc ggctggccta
    23161 tgtcgaggct gtcagtaaat taccgtcaga ttctcctgag tttccgctat gggaatatta
    23221 ttaccgttgc cgcctgctgc aggattatat cagcggtatg accgacctct atgcgtggga
    23281 tgaataccga cgtctgatgg ccgtagaaca ataaccaggc ttttgtaaag acgaacaata
    23341 aatttttacc ttttgcagaa actttagttc ggaacttcgc gctataaaac gaatctgaag
    23401 aacacagcaa ttttgcgtta tctgttaatc gagactgaaa tacatgaaaa aaaccacatt
    23461 agcactgagt gcactggctc tgagtttagg tttggcgtta tctccgctct ctgcaacggc
    23521 ggctgagact tcttcagcaa cgacagccca gcagatgcca agccttgcac cgatgctcga
    23581 aaaggtgatg ccttcagtgg tcagcattaa cgtagaaggt agcacaaccg ttaatacgcc
    23641 gcgtatgccg cgtaatttcc agcagttctt cggtgatgat tctccgttct gccaggaagg
    23701 ttcaccgttc cagagctctc cgttctgcca ggggggccag ggcggtaatg gcggcggcca
    23761 gcaacagaaa ttcatggcgc tgggttccgg cgtcatcatt gatgccgata aaggctatgt
    23821 cgtcaccaac aaccacgttg ttgataacgc gacggtgatt aaagtccagc tgggcgatgg
    23881 tcgtaagttc gacgcgaaga tggttggcaa agatccgcgc tctgatatcg cgctgattca
    23941 gatccagaac ccgaaaaacc tgaccgcaat taagatggcg gattctgatg cactgcgcgt
    24001 gggtgattac accgtagcga ttggtaaccc gtttggcctg ggagagacgg taacttccgg
    24061 gattgtctct gcgctggggc gtagcggcct gaatgccgaa aactacgaaa acttcatcca
    24121 gaccgatgca gcgatcaacc gtggtaactc cggtggtgcg ctggttaacc tgaacggcga
    24181 actgatcggt atcaacaccg cgatcctcgc accggacggc ggcaacatcg gtatcggttt
    24241 tgctatcccg agcaacatgg tgaaaaacct gacctcgcag atggtggaat acggccaggt
    24301 gaaacgcggt gagctgggta ttatggggac tgagctgaat tccgaactgg cgaaagcgat
    24361 gaaagttgac gcccagcgcg gtgctttcgt aagccaggtt ctgcctaatt cctccgctgc
    24421 aaaagcgggc attaaagcgg gtgatgtgat cacctcactg aacggtaagc caatcagcag
    24481 ctttgccgca ctgcgtgctc aggtgggtac tatgccggtg ggcagcaaac tgaccctggg
    24541 cttgctgcgc gacggtaagc aggtcaacgt gaatctggaa ctgcagcaga gcagccagaa
    24601 tcaggttgat tccagctcca tcttcaacgg cattgaaggc gctgagatga gcaacaaagg
    24661 caaagatcag ggcgtggtag tgaacaacgt gaaaacgggc actccggctg cgcagatcgg
    24721 cctgaagaaa ggtgatgtga ttattggcgc gaaccagcag gcagtgaaaa acatcgctga
    24781 actgcgtaaa gttcttgaca gcaaaccgtc tgtactggca ctgaacattc agcgcggcga
    24841 cagctccatc tacctgttaa tgcagtaatc tccctcaacc ccttcctgaa aacgggaagg
    24901 ggttctcctt acaatctgtg aacttcacca caactccata catcttcatc atcctttagg
    24961 catttgcaca atgccgtacg ttacgtactt ccttatgcta agccgtgcat aacggaggac
    25021 ttatggctgg catcttgata ccaaaatggc gcaggatatc gtggcacgta ccatgcgcat
    25081 catcgatacc aatatcaacg taatggatgc ccgtgggcga attatcggca gcggcgatcg
    25141 tgagcgtatt ggtgaattgc acgaaggtgc attgctggta ctttcacagg gacgagtcgt
    25201 cgatatcgat gacgcggtgg cacgtcatct gcacggtgtg cggcaaggga ttaatctacc
    25261 gttacggctg gaaggtgaaa ttgtcggcgt aattggcctg acaggtgaac cagagaatct
    25321 gcgtaaatat ggcgaactgg tctgcatgac ggctgaaatg atgctggaac agtcgcggtt
    25381 gatgcacttg ttggctcagg atagccgttt gcgggaggaa ctggtgatga acctgattca
    25441 ggcagaggag aatactcccg cacttactga atgggcgcaa cggctgggga tcgatctcaa
    25501 tcaaccgcga gtggtggcta ttgttgaggt cgacagcggt cagcttggtg tggacagcgc
    25561 aatggcggag ttacaacaac tgcaaaacgc gctgactacg cccgagcgta ataatctggt
    25621 ggcgattgtc tcgctaaccg aaatggtggt gttgaaaccg gcgttgaact cttttgggcg
    25681 ctgggatgca gaagatcatc gtaagcgagt tgaacaactg attacccgca tgaaagagta
    25741 cggccagctg cgttttcgcg tttcactggg caactatttt accggtcctg gcagtattgc
    25801 ccgatcctat cgtacggcga aaacgacgat ggtggtgggt aaacagcgga tgccagaaag
    25861 tcgctgctat ttttatcagg atctgatgtt acctgtgtta ctcgacagtt tgcgtggcga
    25921 ctggcaggcc aacgaactgg cgcgaccgct ggcgcggctg aaagcgatgg acaataacgg
    25981 cttgctgcga cgaacgctgg cggcgtggtt tcgtcacaat gtgcaaccgc tggcaacgtc
    26041 aaaggcgttg tttattcatc gtaataccct ggagtatcgg cttaatcgta tatcggaact
    26101 gaccgggctt gatttgggca attttgatga caggttgctg ctgtatgtgg cgttacaact
    26161 ggatgaagag cggtaggtta tgcgttaagg ttgaggcggc gctggctcat tcccctcagc
    26221 ccgaagggga gaggggaatg cccgaattaa ccgtcaattc gggattattt acgcgttagt
    26281 ttttccagat cggcttcaat ctcgctgatc ttattggtta cgactgactc caggtgacgt
    26341 aagtcgtcga ggatcttacg cttaagatca acttcgctgc ggtcacgctg gcagatttga
    26401 tcaagctcat caatgatata ccgtagattc gggctgattt cctggacttc tttataaccc
    26461 tgacccacac catcagcgac gaccgtttta cgctgacgcg gatatttaaa cttaacgctc
    26521 ttggcgaaaa actcgccttt gtctttctgg aaatagattt tcagaatatc gttgttggct
    26581 tcctgccgga ggctgtaacg atcaatttct tcaggattgg taatacccag acttttcaga
    26641 ttgtcgtaca tagcgttacc tcaaaatgag tcagtaaatt gtgcttattt tagcatttgg
    26701 cctggcccgc ccggctcgac tgtgatcgca gaaagccttt ttcttccaga cttctatcat
    26761 ggcgtaaaaa tcaaaaaatt acctgcttta ttgtggtgat aaaattcacg ttctacacca
    26821 gttccagcat cgttacttcc gggcggcagt tcagacgcaa accatacaaa ctgcccacgc
    26881 cacgggttgt gtagatgtgt cgttcgccaa aggcatttaa tccggcgacg taacgtttat
    26941 cttcgacagg ggcaaaaggt tcaccaacca acggtacgcg cagttgcccg ccgtgagtat
    27001 ggccgcacag catcagatcc cagggttcat cacgcatgac ttctttgctg tcgggattat
    27061 gcgccagcac cagtcttggc agattggctt cgctggcggg aggcggtttg cattgtccgg
    27121 cccataagtc accggtgcct accagttcga attgcctgtt cggcgtggcg atcaccgtag
    27181 cctggttaaa caacaccgtg atgcccgctg atttcaacgt ctcgccaatt aagtgatttt
    27241 tttctgtacc aacagggcga tcgtggttgc cgaagcaggc aaacgtcggc gcacattcgg
    27301 caagggggga gaggacatca ctaaacgccg aaaaattcag cggcatatca aataatacgt
    27361 aatcgccgcc cagtaatatc aaatcgggct tttgttctat gccaagagca atcgcgtcag
    27421 aaatcaggct taaaggaaca aaacgagagt aatggagatc ggccagaaaa agaattttga
    27481 atggtgctgc gttgtcttta aaaaaggcga ggcggtgacg gattaattca aaccagcctg
    27541 gctcccagta atgcatataa ccgaaacctg agctcgtggc tatcgtcgcg gcagtagcct
    27601 gcaacaatcg gcggcgtgaa atcatcgctc atccctgcaa aaaaaatcgg gcagcgtcgt
    27661 gctgcccgtg tgcatacttt tagtcgatgg tacgcagcag ttcgttaatg ccgactttgc
    27721 cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca gtagaggctg tatttgccat
    27781 cttttgacgg cagattacct gaaacaacca cagaccccgc cggaacgcga ccgtagtgga
    27841 tttcgccggt ttcacggtcg taaatacggg tgctctgacc aatgtatacg cccatggaaa
    27901 tgacggaacc ttcttcgaca atcacccctt caaccacttc agagcgtgcg ccaatgaagc
    27961 agttatcttc aatgatggtt gggttagcct gcagcggttc cagcacgccg ccgatgccca
    28021 cgccaccgga aaggtggacg tttttaccaa tctgcgcaca agaaccgacg gtcgcccagg
    28081 tatcaaccat ggtgccttca tcaacatatg cgccgatgtt gacgtaagac ggcatcagca
    28141 cggtgttacg ggcaataaac gcaccctgac gtaccgccgc tggtggcaca acgcggaagc
    28201 cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt catcggcact ttgtcgaagt
    28261 agcggctttc tgccccttcg atcacctgat tatcattaat acggaaagag agcagcaccg
    28321 cttttttcaa ccactgatgc gtcacccact gaccgtcaat tttttccgct acacgcagtg
    28381 caccggaatc cagcagggcg atcacctgat ttaccgcttc gcgggtaacg gtgtctgcgt
    28441 tggctggcgt gatctcggca cggcgttcaa aagcggtttc aataatgttc tgtaactgct
    28501 gcattgttaa actcttttca tatcagtaaa cacatcaccc tttatcgttt ggattgaggg
    28561 cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt attaagtgca cgccggtcag
    28621 cggtggcaat tatgaataaa tcttctactc gctcgccaat ggttgtaatt cgggcaccat
    28681 gaagcgaaat tcccagatcg gcaaaaattt tcccgactct cgccagcagt cctggctggt
    28741 cgagcgcgat aagctcaagg aacgatttgc ggtcggtatg ggtcggcaaa aacgttactt
    28801 cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg acggggctgc ggtggctgcc
    28861 agctactttg cgtcagtact tgctccagac caaaccgaat aacctcatga cgatctgcgg
    28921 acagcgggct gccatcgggt tccagcacga taaaggtatc catcgccata ccgtcgcgag
    28981 tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg gtctaattcg gcacagacgg
    29041 cggcaaacag ataggggcgg tccgggctcc agataaaaat ctcggtgcct ccacgcgtag
    29101 cctgcgggct aagcaatacc agcggtttgc ttaaatcatg ctgtaataaa tggcgggcat
    29161 gccaggccag ttgatttggg ctatggcgga caaaatagtt agcacgacag cgtgaccaaa
    29221 tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag tagtgccagt gcctggagtt
    29281 ggtgatgacg aacccgttcg cgcatatccg gcgtgttttg cattccgcgt cgtagctgct
    29341 tttcggtggc aaaatagagt tcgcgcaaca gactttgctt ccagctattc cacagcgttt
    29401 cgttggtggc gcaaatgtca gccaccgtca ggcataccag ataacgaagt cggttttctg
    29461 tttgcacttc ttcggcaaac tgcttgatga cttccgggtc ctgaatatcg cggcgttggg
    29521 cggtcaccga catcaacagg tgctggcgaa ccagccaggc aaccagctgt gtttcgcgcg
    29581 agttaagccc gtggagttcg gcaaaatgca ctacatcctg agcaccgaga atggagtggt
    29641 cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc gatgaaaatc agctcagttg
    29701 acggcaggcg cggccagacg tccacacaca acggatggcg ctggcgcgtt tcttcactgg
    29761 caaaactctc cagtttcagc atcacgcgga tagtatgttc atccaccgtg taggcgtgga
    29821 acagatcaaa ctgcatctgc ccgacgatat gcgaccattg cggcatatac gcgccgagca
    29881 cgctatggcg atgcattggc aatagcccgc gccgcaccgc tccggggtga cgcagaatgc
    29941 tcaaaaacag ttttcgtgct tccggaatat tacacagcgg ttgttgcaga tggcgacggg
    30001 catggcgtaa ctggcgcagc gtggtggagt aaatgccggt gatcgcactg ttgcgcacca
    30061 tggtgtagaa catacgcaag atggcttccg gctggcgcat aaatagtgtt tcatcacgca
    30121 ggtcgattag cgtaccgcgt agctgaaact catcgtcgag tggacgtggt ttttcgtcgg
    30181 cgggaagggc gaggatggct tcatcgaaca gttgcagcag catctggttg agttcactga
    30241 cgcggcgtgt aacgcggaag taatccttca tcatccgctc gaccggttcg ttgccttcac
    30301 cactgtaatt cagacgctga gcgacgctaa gttggcgatc gaataacaga cgattatcgt
    30361 aacggctgac gaccagatgc agggcaaagc gaatacgcca cagtatatgc agacattcgt
    30421 ttaattccgc ccgctccgct gaggttaaga agccaaaccc aaccatttca tccagcgatg
    30481 ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg gatatcgcgc aagccgccag
    30541 ggctactttt gatgtctggt tcaagattgt agctggtgcc gtggtaacgc tgatggcgct
    30601 ggttctgttc ttcaactttc gccgcgtaga acttgtcgga aggccagaat ccttcgctaa
    30661 aaatatgttt ttgcagttcg aggaagagcg caacatcgcc aattaataag cgggattcga
    30721 ttaaattggt ggcgacggtt aaatccgata acccttccag catgcactct tcaagcgtgc
    30781 gcacactatg accgacttcc agctttacat cccagagcag cgttaacagc tcgcccactt
    30841 tttgcgcctg atcgtccggg agctttttac ggcttaaaat cagtaaatcg atgtcagaaa
    30901 gtggatgcag ctcgccacga ccgtagccac cgacggcgac caatgccagg tcggcaatct
    30961 ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg gtcgataaac tcggtgcgcg
    31021 cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc atcacccagc caacgctgga
    31081 aagtatcgat atgggctttt atcccaccga cggttaattc atcacggggc cagacgcatg
    31141 gattttgcgg ttgaccgggc agggtgggga gagcggtgtt tgcgtactgt tctggaaggg
    31201 tattcattgt gcgccaccca taagattaaa ttatcgcatt aaaaaagccg gcattcgccg
    31261 gcttcatctt attcgtcgtg cgagattatc gccgggatgg tgtcatcctt gcgtagcgtc
    31321 agaatttcgc agccgttatc agtcaccaca atagtatgct catattgtgc agacaagctg
    31381 cgatctttgg tttttaccgt ccagccatct ttcatggtgc ggatctcttt tttacccgcg
    31441 ttgaccattg gctcgatggt gaacgtcatc ccaggtttca gtacgacgtt ggtttcacgg
    31501 gagtcatagt gcagcacctg cggttcttca tggaagccgc gaccaatacc gtgtccgcaa
    31561 tattcacgaa cgacggagaa gccttctgct tcgacaaatt tctgaatcgc cgcaccgatt
    31621 tcgcgcagat taatgcctgg tttgaccatg cgcagcgcca ggtacaggct ttcttgcgtg
    31681 atgcggcaca gacgttcgcc catgatggtc ggcttaccga cgataaacat tttcgaggta
    31741 tcgccgtgga aaccatcttt gattacggtg acatcaatgt taacgatatc gccatctttc
    31801 agcagcttcg catcgtccgg gataccgtgg cacaccactt cattaataga gatgcaaacg
    31861 gatttcggat agccgtgata gccgaggcag gcagaaaccg tgtgttgttc attaacaatg
    31921 taatcattac agatgcgatc cagctcgccg gtgctgacgc ccggtttaac atacggttcg
    31981 atcatctcca gcacttcggc agccagtcgg ccagcgacgc gcattttttc gatatcttct
    32041 ggggtcttga ttgagatagc cattaattct gtccatcagc gtcggtgata ccgacaatat
    32101 atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt gagaatcatt ctgaatttcg
    32161 ccaaacgtgc cactgaacgt tttctataat agaaaattca acgtctgatg ctgtacacag
    32221 cgccaacaat tattggtgtc catgacgtat ttgtggtata aagcgcgccg gacttccgat
    32281 ccatttcgta tacacagact ggacggaagc gacaatctca ctttgtgtaa caacacacac
    32341 gtatcggcac atattccggg gtgccctttg gggtcggtaa tatgggatac gtggaggcat
    32401 aaccccaact tttatataga ggttttaatc atggcaactg tttccatgcg cgacatgctc
    32461 aaggctggtg ttcacttcgg tcaccagacc cgttactgga acccgaaaat gaagccgttc
    32521 atcttcggtg cgcgtaacaa agttcacatc atcaaccttg agaaaactgt accgatgttc
    32581 aacgaagctc tggctgaact gaacaagatt gcttctcgca aaggtaaaat ccttttcgtt
    32641 ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg ctctgagctg cgaccagttc
    32701 ttcgtgaacc atcgctggct gggcggtatg ctgactaact ggaaaaccgt tcgtcagtcc
    32761 atcaaacgtc tgaaagacct ggaaactcag tctcaggacg gtactttcga aaagctgacc
    32821 aagaaagaag cgctgatgcg cactcgtgag ctggagaaac tggaaaacag cctgggcggt
    32881 atcaaagaca tgggcggtct gccggacgct ctgtttgtaa tcgatgctga ccacgaacac
    32941 attgctatca aagaagcaaa caacctgggt attccggtat ttgctatcgt tgataccaac
    33001 tctgatccgg acggtgttga cttcgttatc ccgggtaacg acgacgcaat ccgtgctgtg
    33061 accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag gccgttctca ggatctggct
    33121 tcccaggcgg aagaaagctt cgtagaagct gagtaataag gcttgataac tcccccaaaa
    33181 tagttcgagt tgcagaaagg cggcaagctc gagaattccc gggagcttac atcagtaagt
    33241 gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg aaatatgaag ggggagagcc
    33301 cttatagacc aggtagtaca cgtttggtta gggggcctgc atatggcccc ctttttcact
    33361 tttatatctg tgcggtttaa tgccgggcag atcacatctc cgaggatttt agaatggctg
    33421 aaattaccgc atccctggta aaagagctgc gtgagcgtac tggcgcaggc atgatggatt
    33481 gcaaaaaagc actgactgaa gctaacggcg acatcgagct ggcaatcgaa aacatgcgta
    33541 agtccggtgc tattaaagca gcgaaaaaag caggcaacgt tgctgctgac ggcgtgatca
    33601 aaaccaaaat cgacggcaac tacggcatca ttctggaagt taactgccag actgacttcg
    33661 ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt tctggacgca gctgttgctg
    33721 gcaaaatcac tgacgttgaa gttctgaaag cacagttcga agaagaacgt gttgcgctgg
    33781 tagcgaaaat tggtgagaac atcaacattc gccgcgttgc tgcgctggaa ggcgacgttc
    33841 tgggttctta tcagcacggt gcgcgtatcg gcgttctggt tgctgctaaa ggcgctgacg
    33901 aagagctggt taaacacatc gctatgcacg ttgctgcaag caagccagaa ttcatcaaac
    33961 cagaagacgt atccgctgaa gtggtagaaa aagaatacca ggtacagctg gatatcgcga
    34021 tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt tgaaggccgc atgaagaaat
    34081 tcaccggcga agtttctctg accggtcagc cgttcgttat ggaaccaagc aaaactgttg
    34141 gtcagctgct gaaagagcat aacgctgaag tgactggctt catccgcttc gaagtgggtg
    34201 aaggcatcga gaaagttgag actgactttg cagcagaagt tgctgcgatg tccaagcagt
    34261 cttaattatc aaaaaggagc cgcctgaggg cggcttcttt ttgtgcccat cttgtaaatt
    34321 cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca atgtcgctag tattaattca
    34381 tttcaatcgt tgacagtctc aggaaagaaa catggctacc aatgcaaaac ccgtctataa
    34441 acgcattctg cttaagttga gtggcgaagc tctgcagggc actgaaggct tcggtattga
    34501 tgcaagcata ctggatcgta tggctcagga aatcaaagaa ctggttgaac tgggtattca
    34561 ggttggtgtg gtgattggtg ggggtaacct gttccgtggc gctggtctgg cgaaagcggg
    34621 tatgaaccgc gttgtgggcg accacatggg gatgctggcg accgtaatga acggcctggc
    34681 aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt ctgatgtccg ctattccatt
    34741 gaatggcgtg tgcgacagct acagctgggc agaagctatc agcctgttgc gcaacaaccg
    34801 tgtggtgatc ctctctgccg gtacaggtaa cccgttcttt accaccgact cagcagcttg
    34861 cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa gcaaccaaag ttgacggcgt
    34921 gtttaccgct gatccggcga aagatccaac cgcaaccatg tacgagcaac tgacttacag
    34981 cgaagtgctg gaaaaagagc tgaaagtcat ggacctagcg gccttcacgc tggctcgtga
    35041 ccataaatta ccgattcgtg ttttcaatat gaacaaaccg ggtgcgctgc gccgtgtggt
    35101 aatgggtgaa aaagaaggga ctttaatcac ggaataattc ccgtgatgga taaataaggg
    35161 taagattccg cgtaagtatc gcgggggcgt tagtctggtt ataaggcgtt attgttgcag
    35221 gcagtttggt cacggccagc gcgcagcaac cggagcgtac aaaagtacgt gaggatggcg
    35281 agcactgctc ggggccaaaa tggcaaataa aatagcctaa taatccagac gattacccgt
    35341 aatatgttta atcagggcta tacttagcac acttccactg tgtgtgactg tctggtctga
    35401 ctgagacaag ttttcaagga ttcgtaacgt gattagcgat atcagaaaag atgctgaagt
    35461 acgcatggac aaatgcgtag aagcgttcaa aacccaaatc agcaaaatac gcacgggtcg
    35521 tgcttctccc agcctgctgg atggcattgt cgtggaatat tacggcacgc cgacgccgct
    35581 gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtaca ctgaaaatca acgtgtttga
    35641 tcgttcaatg tctccggccg ttgaaaaagc gattatggcg tccgatcttg gcctgaaccc
    35701 gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg ctgacggaag aacgtcgtaa
    35761 agatctgacc aaaatcgttc gtggtgaagc agaacaagcg cgtgttgcag tacgtaacgt
    35821 gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa gataaagaga tcagcgaaga
    35881 cgacgatcgc cgttctcagg acgatgtaca gaaactgact gatgctgcaa tcaagaaaat
    35941 tgaagcggcg ctggcagaca aagaagcaga actgatgcag ttctgatttc ttgaacgaca
    36001 aaaacgccgc tcagtagatc cttgcggatc ggctggcggc gttttgcttt ttattctgtc
    36061 tcaactctgg atgtttcatg aagcaactca ccattctggg ctcgaccggc tcgattggtt
    36121 gcagcacgct ggacgtggtg cgccataatc ccgaacactt ccgcgtagtt gcgctggtgg
    36181 caggcaaaaa tgtcactcgc atggtagaac agtgcctgga attctctccc cgctatgccg
    36241 taatggacga tgaagcgagt gcgaaacttc ttaaaacgat gctacagcaa cagggtagcc
    36301 gcaccgaagt cttaagtggg caacaagccg cttgcgatat ggcagcgctt gaggatgttg
    36361 atcaggtgat ggcagccatt gttggcgctg ctgggctgtt acctacgctt gctgcgatcc
    36421 gcgcgggtaa aaccattttg ctggccaata aagaatcact ggttacctgc ggacgtctgt
    36481 ttatggacgc cgtaaagcag agcaaagcgc aattgttacc ggtcgatagc gaacataacg
    36541 ccatttttca gagtttaccg caacctatcc agcataatct gggatacgct gaccttgagc
    36601 aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg ccctttccgt gagacgccat
    36661 tgcgcgattt ggcaacaatg acgccggatc aagcctgccg tcatccgaac tggtcgatgg
    36721 ggcgtaaaat ttctgtcgat tcggctacca tgatgaacaa aggtctggaa tacattgaag
    36781 cgcgttggct gtttaacgcc agcgccagcc agatggaagt gctgattcac ccgcagtcag
    36841 tgattcactc aatggtgcgc tatcaggacg gcagtgttct ggcgcagctg ggggaaccgg
    36901 atatgcgtac gccaattgcc cacaccatgg catggccgaa tcgcgtgaac tctggcgtga
    36961 agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc cgcaccggat tatgatcgtt
    37021 atccatgcct gaaactggcg atggaggcgt tcgaacaagg tcaggcagcg acgacagcat
    37081 tgaatgccgc aaacgaaatc accgttgctg cttttcttgc gcaacaaatc cgctttacgg
    37141 atatcgctgc gttgaattta tccgtactgg aaaaaatgga tatgcgcgaa ccacaatgtg
    37201 tggacgatgt gttatctgtt gatgcgaacg cgcgtgaagt cgccagaaaa gaggtgatgc
    37261 gtctcgcaag ctgaggataa tccggctaca gagagtcgcg ctatttgtta gcgtagggct
    37321 tcagtgatat agtctgcgcc atctgatcgt aagtagttgg ctttataagg tcaggtatgc
    37381 cgtggtttta cacggctttt ttttgtatag gcttcagtat tcctgagtac cgtaaaccct
    37441 gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa ccacttagcg aaaaattgcc
    37501 agcgcatggc tgccgtcatg ttgcgatcat tatggacggc aatggtcgct gggcaaaaaa
    37561 gcaagggaag attcgtgcct ttgggcataa agccggggca aaatccgtcc gccgggctgt
    37621 ctcttttgcg gccaacaacg gtattgaggc gttaacgctg tatgccttta gtagtgaaaa
    37681 ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa ctgtttgtgt gggcgctcga
    37741 tagcgaagta aaaagtctgc accgacataa cgtgcgtctg cgtattattg gcgataccag
    37801 tcgctttaac tcgcgtttgc aagaacgtat tcgtaaatct gaagcgctaa cagccgggaa
    37861 tagcggtctg acgctgaata ttgcggcgaa ctacggtgga cgttgggata tagtccaggg
    37921 agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg caaccagatc agatagatga
    37981 agagatgcta aaccagcatg tctgtatgca tgaactggcc cctgtagatt tagtaattag
    38041 gactgggggg gagcatcgca ttagtaactt tttgctttgg caaattgcct atgccgaact
    38101 ttactttaca gatgttctct ggcccgattt cgatgaacaa gactttgaag gggcgttaaa
    38161 tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag cccggtgatg aaacagcctg
    38221 atgggggtcg cttttgctga agtatcgcct gatatctgct tttgtgttaa tacccgtcgt
    38281 catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc atcgtaacgc tggtggtctg
    38341 catgctggca gcgtgggaat ggggacagct tagcggtttt accactcgtt cgcagcgagt
    38401 atggttggcg gtgttatgcg ggttattgtt ggcgctgatg ctttttctgt tgccggaata
    38461 tcaccgaaat attcatcaac cgctggttga aatctcactt tgggcttcgt tgggttggtg
    38521 gattgtcgcg ctattgctgg tgctgtttta cccaggttcc gcagcaatct ggcgtaactc
    38581 taaaacattg cgccttattt ttggcgtgct aaccattgtt cccttcttct ggggcatgct
    38641 ggcgttacgg gcctggcact acgacgagaa tcattacagt ggcgcaatat ggctgctcta
    38701 tgtcatgatc ctggtatggg gcgctgactc tggcgcatat atgtttggca aattgtttgg
    38761 taaacataag ctggcaccga aggtttctcc gggtaaaacc tggcaaggct ttataggtgg
    38821 actagcgacc gcagcggtaa tctcatgggg ttatggcatg tgggcgaatc tcgacgtcgc
    38881 tcccgtcacc ttactcattt gctctattgt cgcagcgtta gcctcagtgc tcggcgatct
    38941 gaccgagagt atgtttaagc gtgaagcagg aattaaggac agcggtcatt taattccagg
    39001 acacggtggt attttagatc gtattgatag cctgacggct gcggtaccgg tctttgcttg
    39061 cttgttgtta ctggtattca ggacgcttta acggaaggta atatgctgag ttttctctgg
    39121 gatttggctt cgttcatcgt tgcactgggt gtacttatca ccgtgcatga atttggtcat
    39181 ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt tctcaatagg gtttggtaag
    39241 gcgctctggc ggcgaactga taagctcggc accgaatatg ttatcgccct gatcccgctg
    39301 ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg tcgttccgga actccgccac
    39361 catgccttca ataataaatc tgtcggccaa cgagcggcga ttattgccgc aggtccggtt
    39421 gcaaacttca tttttgctat ctttgcctac tggctggttt ttattattgg tgtgcctggc
    39481 gtacgtccgg tggttggtga aatagcagcc aattcgatag ctgcggaagc acaaattgca
    39541 ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc ctgattggga tgccgtgcgt
    39601 ttgcagttgg tcgataaaat tggcgatgaa agcaccacca ttacagtagc gccatttggc
    39661 agcgaccaac ggcgggatgt aaagctcgat ttacgtcact gggcgtttga gcctgataaa
    39721 gaagatccgg tatcttcgct ggggattcgt cctcgtgggc cgcaaattga acctgtactg
    39781 gaaaatgtgc aaccaaactc ggcggcaagc aaggcaggtt tgcaagcagg cgacaggatc
    39841 gttaaagtcg atggtcagcc cttaacgcag tgggtgacct ttgtgatgct tgtccgggat
    39901 aatccgggta aatccttagc gttagaaatc gaaaggcagg gaagtccctt gtctttgaca
    39961 ttaatcccgg agagtaaacc gggtaatggt aaagcgattg gttttgtcgg tattgagccg
    40021 aaagtcattc ctttgccaga tgagtataaa gttgtacgcc agtatgggcc gttcaacgcc
    40081 atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc tgacggtcag tatgctggga
    40141 aaattgatca ccggtgatgt gaaactgaac aacctcagtg ggccgatctc tatcgccaag
    40201 ggggctggga tgacagcgga actcggggta gtttattacc tgccgtttct tgcgcttatt
    40261 agcgtgaact tagggataat taacctgttt ccgttgcccg tacttgacgg ggggcatctg
    40321 ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat ccgagcgggt tcaagacttt
    40381 tgttatcgca ttggctcgat tctgctggtg ctgttaatgg ggcttgcact tttcaatgat
    40441 ttctctcggt tatgagagtt agttaggaag aacgcataat aacgatggcg atgaaaaagt
    40501 tgctcatagc gtcgctgctg tttagcagcg ccaccgtata cggtgctgaa gggttcgtag
    40561 tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt tggtgcggcc ctcctcagta
    40621 tgccggtgcg cacaggcgac acggttaatg atgaagatat cagtaatacc attcgcgctc
    40681 tgtttgctac cggcaacttt gaggatgttc gcgtccttcg tgatggtgat acccttctgg
    40741 ttcaggtaaa agaacgtccg accattgcca gcattacttt ctccggtaac aaatcggtga
    40801 aagatgacat gctgaagcaa aacctcgagg cttctggtgt gcgtgtgggc gaatccctcg
    40861 atcgcaccac cattgccgat atcgagaaag gtctggaaga cttctactac agcgtcggta
    40921 aatatagcgc cagcgtaaaa gctgtcgtga ccccgctgcc gcgcaaccgt gttgacctaa
    40981 aactggtgtt ccaggaaggt gtgtcagctg aaatccagca aattaacatt gttggtaacc
    41041 atgctttcac caccgacgaa ctgatctctc atttccaact gcgtgacgaa gtgccgtggt
    41101 ggaacgtggt aggcgatcgt aaataccaga aacagaaact ggcgggcgac cttgaaaccc
    41161 tgcgcagcta ctatctggat cgcggttatg cccgtttcaa catcgactct acccaggtca
    41221 gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa cattaccgaa ggcgatcagt
    41281 acaagctttc tggcgttgaa gtgagcggca accttgccgg gcactccgct gaaattgagc
    41341 agctgactaa gatcgagccg ggcgagctgt ataacggcac caaagtgacc aagatggaag
    41401 atgacatcaa aaagcttctc ggtcgctatg gttatgccta tccgcgcgta cagtcgatgc
    41461 ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt gaacgttgat gcgggtaacc
    41521 gtttctacgt gcgtaagatc cgttttgaag gtaacgatac ctcgaaagat gccgtcctgc
    41581 gtcgcgaaat gcgtcagatg gaaggtgcat ggctgggaag cgatctggtc gatcagggta
    41641 aggagcgtct gaatcgtctg ggcttctttg aaactgtcga taccgatacc caacgtgttc
    41701 cgggtagccc ggaccaggta gatgtcgtct acaaggtaaa agagcgcaac accggtagct
    41761 tcaacttcgg gattggttac ggtactgaaa gtggcgtgag cttccaggct ggtgtgcagc
    41821 aggataactg gttaggtaca ggttatgctg ttggtatcaa cgggaccaaa aacgattacc
    41881 agacctatgc tgaactgtcg gtaaccaacc cgtacttcac tgtagatggt gtaagcctcg
    41941 gtggtcgtct cttctataat gacttccagg cagatgacgc cgacctgtcc gactatacca
    42001 acaagagtta tggtacagac gtgacgttgg gcttcccgat caacgaatat aactcgctgc
    42061 gtgcaggtct gggttatgta cataactccc tgtccaacat gcagcctcag gttgcgatgt
    42121 ggcgttatct gtactctatg ggtgaacatc cgagcacctc tgatcaggat aatagcttca
    42181 aaacggacga cttcacgttc aactatggtt ggacctataa caagcttgac cgtggttact
    42241 tcccgacaga tggttcacgt gtcaacctga ccggtaaagt gaccattcct ggatcggata
    42301 acgaatacta caaagtgacg ttagacacgg cgacttatgt gccgatcgat gacgatcaca
    42361 aatgggttgt tctggggcgt acccgctggg gttatggtga tggtttaggc ggcaaagaga
    42421 tgccgttcta cgagaacttc tatgccggtg gttccagcac cgtgcgtggc ttccagtcca
    42481 atacaattgg tccgaaagca gtttacttcc cgcatcaggc cagtaattat gatccggact
    42541 atgattacga atgtgcgact caggacggcg cgaaagacct gtgtaaatcg gatgatgctg
    42601 taggcggtaa cgccatggcg gttgccagcc tcgagttcat caccccgacg ccgtttatta
    42661 gcgataagta tgctaactcg gttcgtactt ccttcttctg ggatatgggt accgtttggg
    42721 atacaaactg ggattccagc caatattctg gatatccgga ctatagtgat ccaagcaata
    42781 tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc attggggccg ttggtgttct
    42841 cctacgccca gccgttcaaa aagtacgatg gagacaaggc agaacagttc cagtttaaca
    42901 tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag tggtagtgta gcgatgactt
    42961 taggcgatca atataagatc gccgggccac gcaaagaact gcaccctccg gtgcaaatgg
    43021 gatggtaagg agtttattgt gaaaaagtgg ttattagcgg caggtctcgg tttagcactg
    43081 gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca acatgggcag cctgttccag
    43141 caggtagcgc agaaaaccgg tgtttctaac acgctggaaa atgagttcaa aggccgtgcc
    43201 agcgaactgc agcgtatgga aaccgatctg caggctaaaa tgaaaaagct gcagtccatg
    43261 aaagcgggca gcgatcgcac taagctggaa aaagacgtga tggctcagcg ccagactttt
    43321 gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc gttccaacga agaacgcggc
    43381 aaactggtta ctcgtatcca gactgctgtg aaatccgttg ccaacagcca ggatatcgat
    43441 ctggttgttg atgcaaacgc cgttgcttac aacagcagcg atgtaaaaga catcactgcc
    43501 gacgtactga aacaggttaa ataagtaatg ccttcaattc gactggctga tttagcgcag
    43561 cagttggatg cagaactaca cggtgatggc gatatcgtca tcaccggcgt tgcgtccatg
    43621 caatctgcac aaacaggtca cattacgttc atggttaacc caaaataccg tgagcattta
    43681 ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg atcttccttt cgcgaaaagt
    43741 gccgcgctgg tagtgaagaa tccctacctg acttacgcgc gcatggcgca aattttagat
    43801 accacgccgc agcccgcgca gaacattgca cccagtgcgg tgatcgacgc gacggcgaag
    43861 ctgggtaaca acgtatcgat tggcgctaac gcggtgattg agtccggcgt tgaactgggc
    43921 gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa acagcaaaat cggtgcaggt
    43981 tcgcgtctct gggcgaacgt aaccatttac catgagatcc agatcggtca gaattgcctg
    44041 atccagtccg gaacagtggt aggcgcagac ggctttggtt atgccaacga tcgtggtaac
    44101 tgggtgaaga tcccacagat tggtcgcgta attattggcg atcgcgtgga gatcggtgcc
    44161 tgcacaacca tcgatcgcgg cgcgctggat gacactatta ttggcaatgg cgtgatcatt
    44221 gataaccagt gccagattgc acataacgtc gtgattggcg acaatacggc ggttgccggt
    44281 ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact gcatgatcgg cggagccagc
    44341 gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg ttacgggcat gggtatggtg
    44401 atgcgtccca tcactgaacc aggcgtctat tcctcaggca ttccgctgca acccaacaaa
    44461 gtctggcgca aaaccgctgc actggtgatg aacattgatg acatgagcaa gcgtctgaaa
    44521 tcgcttgagc gcaaggttaa tcaacaagac taacgttcca tcttttgttc gccaaacttt
    44581 acggcctgtc tcattcttac gattgcggca ggccgtgtta ttattgtcgt ttcttatatt
    44641 ttgacaggaa gagtatcttg actactaaca ctcatactct gcagattgaa gagattttag
    44701 aacttctgcc gcaccgtttc ccgttcttac tggtggatcg cgtgctggat tttgaagaag
    44761 gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga gccattcttc cagggccatt
    44821 tccctggaaa accgattttc ccgggtgtgc tgattctgga agcaatggca caggcaacag
    44881 gtattctggc gtttaaaagc gtaggaaaac tggaaccggg tgagctgtac tacttcgctg
    44941 gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg cgatcaaatg atcatggaag
    45001 tcactttcga aaaaacgcgc cgcggcctga cccgttttaa aggggttgct ctggtcgatg
    45061 gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag ccgggaggcc tgatacgtga
    45121 ttgataaatc cgcctttgtg catccaaccg ccattgtgga agagggcgcg tcaattggcg
    45181 cgaacgcaca cattggtcct ttttgtatcg ttggacccca tgtcgaaatt ggtgagggta
    45241 ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa aattggccgc gataatgaga
    45301 tttatcagtt cgcctccatc ggcgaagtta accaggatct gaaatatgct ggcgaaccga
    45361 cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag cgtcaccatt catcgtggca
    45421 cagtccaggg cggtggattg acgaaggtgg gcagcgacaa cttactgatg atcaacgcgc
    45481 acattgcgca cgattgtacg gtaggtaacc gctgtattct cgccaacaac gcaacgctgg
    45541 cgggtcacgt atcggttgac gacttcgcga tcatcggcgg catgaccgca gtccatcagt
    45601 tctgcatcat tggtgcgcac gtgatggttg gcggctgctc cggtgtggcg caggacgtcc
    45661 ctccttatgt cattgcgcag ggtaaccacg caacgccgtt cggtgtcaat atcgaagggc
    45721 tgaagcgccg cggattcagc cgtgaggcga ttaccgctat ccgcaatgcg tataagctga
    45781 tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga aattgctgaa ctggcggaaa
    45841 catatccgga agtgaaagcc tttaccgatt tctttgcacg ctcaacgcgc ggtctgattc
    45901 gttaatgact gaacagcgtc cattaacgat tgccctggtc gccggagaaa cctccggcga
    45961 tatcctgggg gccggtttaa tccgcgctct gaaagaacgt gtgcccaacg cccgctttgt
    46021 tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa gcctggtacg aaatggaaga
    46081 actggcggtg atgggcattg ttgaagtgct cggtcgtctg cgtcgcttac tgcatattcg
    46141 tgccgatctg acaaagcgtt ttggcgaact gaagccagat atttttgttg gtattgatgc
    46201 gcctgacttc aatattactc ttgaaggtaa cctcaaaaag cagggtatca aaaccattca
    46261 ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc gttttcaaaa taggcagagc
    46321 caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg ttttatgaca aatacaatgt
    46381 accgtgccgc tttatcggtc ataccatggc tgatgccatg ccgttagatc cagataaaaa
    46441 tgccgcccgt gatgtgctgg ggatccctca cgatgcccac tgcctggcgt tgctaccagg
    46501 gagccgtggt gcagaagttg aaatgcttag tgccgatttc ctgaaaacgg cccagctttt
    46561 gcgccagaca tatccggatc tcgaaatcgt ggtgccactg gtgaatgcca aacgccgcga
    46621 gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctt tcagttcatt tgctggatgg
    46681 gatgggccgt gaggcgatgg tcgccagcga tgcggcgcta ctggcgtcgg gtacggcagc
    46741 cctggagtgt atgctggcga aatgcccgat ggtggtggga tatcgcatga agccttttac
    46801 cttctggttg gcgaagcggc tggtgaaaac tgattatgtc tcgctgccaa atctgctggc
    46861 gggtagagag ttagtcaaag aattattgca ggaagagtgt gagccgcaaa aactggctgc
    46921 ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac gcgatgcacg ataccttccg
    46981 tgaactgcat cagcagatcc gctgcaatgc cgatgagcag gcggcacaag ccgttctgga
    47041 gttagcacaa tgatcgaatt tgtttatccg cacacgcagc tggttgcggg tgtggatgaa
    47101 gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg cggtgatcct tgacccggcg
    47161 cgcccgattg ccgggctgaa tgattccaaa aagctgagcg aaaaacgccg tctggcgctc
    47221 tatgaagaga tcaaagagaa agcgttgagc tggagtctgg gccgcgcgga accccacgaa
    47281 atcgacgagc tgaacattct tcatgcgacc atgctggcga tgcagcgtgc cgtcgctggg
    47341 ctgcatattg cgccggaata tgtgttgatt gatggtaacc gctgcccgaa attaccgatg
    47401 cctgcgatgg ctgtggtgaa aggcgatagc cgcgtaccgg aaatcagtgc cgcgtctatc
    47461 ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg atattgtttt cccgcaatat
    47521 ggttttgccc aacacaaagg gtacccaacc gcttttcatc tggaaaaact ggctgaacac
    47581 ggcgcgaccg aacaccatcg gcgcagcttt gggcctgtca aacgcgcact gggacttgcg
    47641 tcctgattct tgtgtcgaga ttaagtaaac cggaatctga agatgtctga accacgtttc
    47701 gtacacctgc gggtgcacag cgactactcg atgatcgatg gcctggccaa aaccgcaccg
    47761 ttggtaaaaa aggcggcggc gttgggtatg ccagcactgg cgatcaccga tttcaccaac
    47821 ctttgtggtc tggtgaagtt ctacggagcg ggacatggcg cagggattaa gcctatcgtc
    47881 ggggcagatt ttaacgtcca gtgcgacctg ctgggtgatg agttaaccca cctgacggta
    47941 ctggcggcga acaataccgg ctatcagaat ctgacgttgc tgatctcaaa agcgtatcag
    48001 cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact ggcttatcga attaaacgaa
    48061 gggttgatcc ttctttccgg cggacgcatg ggcgacgtcg gacgcagtct tttgcgtggt
    48121 aacagagcgc tggtagatga gtgtgtcgcg ttttatgaag aacacttccc ggatcgctat
    48181 tttctcgagc tgatccgcac cggcaggccg gatgaagaaa gctatctgca cgcggcggtg
    48241 gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacca acgacgtgcg ctttatcgac
    48301 agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc acgacggctt taccctcgac
    48361 gatcctaaac gcccgcgtaa ctattcgccg cagcaatata tgcgtagcga agaggagatg
    48421 tgtgagctgt ttgccgacat ccccgaagcc cttgccaaca ccgttgagat cgccaaacgc
    48481 tgtaacgtaa ccgtgcgtct tggtgaatac ttcctgccgc agttcccgac cggggacatg
    48541 agcaccgaag attatctggt caagcgtgca aaagagggcc tggaagagcg tctggccttt
    48601 ttattccctg acgaggaaga acgtgttaag cgccgcccgg aatatgacga acgtctggag
    48661 actgaacttc aggttatcaa ccagatgggc ttcccgggct acttcctcat cgttatggaa
    48721 tttatccagt ggtcgaaaga taacggcgta ccggtagggc caggccgtgg ctccggtgcg
    48781 ggttcactgg tggcctacgc gctgaaaatc accgacctcg atccgctgga atttgacctg
    48841 ctgttcgaac gtttccttaa cccggaacgt gtctccatgc ctgacttcga cgttgacttc
    48901 tgtatggaga aacgcgatca ggttatcgag cacgtagcgg acatgtacgg tcgtgatgcg
    48961 gtatcgcaga tcatcacctt cggtacaatg gcggcgaaag cggtgatccg cgacgtaggc
    49021 cgcgtgctgg ggcatccgta cggctttgtc gatcgtatct cgaaactgat cccgcccgat
    49081 ccggggatga cgctggcgaa agcgtttgaa gccgagccgc agctgccgga aatctacgaa
    49141 gcggatgaag aagttaaggc gctgatcgac atggcgcgca aactggaagg ggtcacccgt
    49201 aacgccggta agcacgccgg tggggtggtt atcgcgccga ccaaaattac cgattttgcg
    49261 ccgctttact gcgatgaaga gggcaaacat ccggtcaccc agtttgataa aagcgacgtt
    49321 gaatacgccg gactggtgaa gttcgacttc cttggtttgc gtacgctcac catcatcaac
    49381 tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg gcgagccgcc gctggatatc
    49441 gctgcgatcc cgctggatga taagaaaagc ttcgacatgc tgcaacgctc ggaaaccacg
    49501 gcggtattcc agcttgaatc gcgcggcatg aaggacctga tcaagcgtct acaacctgac
    49561 tgcttcgaag atatgatcgc cctagtggca ctgttccgcc ccggtccgtt gcaatcaggg
    49621 atggtggata actttatcga ccgtaaacat ggtcgtgaag agatctccta tccggacgta
    49681 cagtggcagc atgaaagcct gaaaccggta ctggagccaa cctacggcat tatcctgtat
    49741 caggaacagg tcatgcagat tgcgcaggtg ctttctggtt ataccctcgg tggcgcggat
    49801 atgctgcgtc gtgcgatggg taagaaaaag ccggaagaga tggctaagca acgttctgta
    49861 tttgctgaag gtgcagaaaa gaacggaatc aacgctgaac tggcgatgaa aatcttcgac
    49921 ctggtggaga aattcgctgg ttacggattt aacaaatcgc actctgcggc ctatgctttg
    49981 gtgtcatatc aaacgttatg gctgaaagca cactatcctg cggagtttat ggcggcggta
    50041 atgaccgccg atatggacaa caccgagaag gtggtgggtc tggtggatga gtgctggcgg
    50101 atggggctga aaatcctgcc accagatata aactccggtc tttaccattt ccacgtcaac
    50161 gacgacggcg aaatcgtgta tggtattggc gcgatcaaag gggtcggtga aggtccgatt
    50221 gaggccatca tcgaagcccg taataaaggc ggctacttcc gcgaactgtt tgatctctgc
    50281 gcccgtaccg acaccaaaaa gttgaaccgt cgcgtgctgg aaaaactgat catgtccggg
    50341 gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga actcgctggg tgatgcgtta
    50401 aaagcggcag atcaacacgc gaaagcggaa gctatcggtc aggccgatat gttcggcgtg
    50461 ctggccgaag agccggaaca aattgaacaa tcctacgcca gctgccaacc gtggccggag
    50521 caggtggtat tagatgggga acgtgaaacg ttaggcctgt acctgaccgg acaccctatc
    50581 aaccagtatt taaaagagat tgagcgttat gtcggaggcg taaggctgaa agacatgcac
    50641 ccgacagaac gtggtaaagt catcacggct gcggggctcg ttgttgccgc gcgggttatg
    50701 gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg atgaccgttc cgggcggctg
    50761 gaagtgatgt tgtttactga cgccctggat aaataccagc aattgctgga aaaagaccgc
    50821 atacttatcg tcagcggaca ggtcagcttt gatgacttca gcggtgggct taaaatgacc
    50881 gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat atgctcgcgg gcttgctatc
    50941 tcgctgacgg acaggcaaat tgatgaccag cttttaaacc gactccgtca gtctctggaa
    51001 ccccaccgct ctgggacaat tccagtacat ctctactatc agagggcgga tgcacgcgcg
    51061 cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg atcgtttatt aaacgatctc
    51121 cgtggcctca ttggttcgga gcaggtggaa ctggagtttg actaatacag gaatactatg
    51181 agtctgaatt tccttgattt tgaacagccg attgcagagc tggaagcgaa aatcgattct
    51241 ctgactgcgg ttagccgtca ggatgagaaa ctggatatta acatcgatga agaagtgcat
    51301 cgtctgcgtg aaaaaagcgt agaactgacg cgtaaaatct tcgccgatct tggtgcatgg
    51361 cagattgcgc aactggcacg ccatccacag cgtccttata ccctggatta cgttcgcctg
    51421 gcatttgatg aatttgacga actggctggc gaccgcgcgt atgcagacga taaagctatc
    51481 gtcggtggta tcgcccgtct tgacggtcgt ccggtgatga tcattggtca tcaaaaaggt
    51541 cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc cagcgccaga aggttaccgc
    51601 aaagcactgc gtctgatgca aatggctgaa cgctttaaga tgcctatcat cacctttatc
    51661 gacaccccgg gggcttatcc gggcgtgggc gcagaagaac gtggtcagtc tgaagccatt
    51721 gcacgcaacc tgcgtgaaat gtctcgcctc ggtgtaccgg tagtttgtac ggttatcggt
    51781 gaaggtggtt ctggcggcgc gctggcgatt ggcgtgggcg ataaagtgaa tatgctgcaa
    51841 tacagcacct attccgttat ctcgccggaa ggttgtgcgt ccattctgtg gaagagcgct
    51901 gacaaagcgc cgctggcagc tgaagcgatg ggtatcatcg ctccgcgtct gaaagaacta
    51961 aaactgatcg actccatcat cccggaaccg ctgggtggtg ctcaccgtaa cccggaagcg
    52021 atggcggcat cgttgaaagc gcaactgctg gcggatctgg ccgatctcga cgtgttaagc
    52081 actgaagatt taaaaaatcg tcgttatcag cgcctgatga gctacggtta cgcgtaattc
    52141 gcaaaagttc tgaaaaaggg tcacttcggt ggcccttttt tatcgccacg gtttgagcag
    52201 gctatgatta aggaaggatt ttccaggagg aacacatgaa catcattgcc attatgggac
    52261 cgcatggcgt cttttataaa gatgagccca tcaaagaact ggagtcggcg ctggtggcgc
    52321 aaggctttca gattatctgg ccacaaaaca gcgttgattt gctgaaattt atcgaacata
    52381 accctcgaat ttgcggcgtg atttttgact gggatgagta cagtctcgat ttatgcagcg
    52441 atatcaatca acttaatgaa tatctcccgc tttatgcctt catcaacacc cactcgacga
    52501 tggatgtcag cgtgcaggat atgcggatgg cgctctggtt ttttgaatat gcgctggggc
    52561 aggcggaaga tatcgccatt cgtatgcgtc agtacaccga cgaatatctt gataacatta
    52621 caccgccgtt cacgaaagcc ttgtttacct acgtcaaaga gcggaagtac accttttgta
    52681 cgccggggca tatgggcggc accgcatatc aaaaaagccc ggttggctgc ctgttttatg
    52741 attttttcgg cgggaatacc ctcaaggctg atgtctctat ttcggtcacc gagcttggtt
    52801 cgttgctcga ccacaccggg ccacacctgg aagcggaaga gtacatcgcg cggacttttg
    52861 gcgcggaaca gagttatatc gttaccaacg gaacatcgac gtcgaacaaa attgtgggta
    52921 tgtacgccgc gccatccggc agtacgctgt tgatcgaccg caattgtcat aaatcgctgg
    52981 cgcatctgtt gatgatgaac gatgtagtgc cagtctggct gaaaccgacg cgtaatgcgt
    53041 tggggattct tggtgggatc ccgcgccgtg aatttactcg cgacagcatc gaagagaaag
    53101 tcgctgccac cacgcaggca caatggccgg ttcatgcggt gattaccaac tccacctatg
    53161 atggcttgct ctacaacacc gactggatca aacagacgct ggatgtcccg tcgatccact
    53221 tcgattccgc ctgggtgccg tacacccatt ttcatccgat ctaccagggg aaaagtggta
    53281 tgagcggcga gcgtgttgcg ggaaaagtga tcttcgaaac gcaatcgacc cacaaaatgc
    53341 tggcggcgtt atcgcaggct tcgctgatcc acattaaagg cgagtatgac gaagaggcgt
    53401 ttaacgaagc ctttatgatg cataccacca cctcgcccag ttatcccatt gttgcttcgg
    53461 ttgagacggc ggcggcgatg ctgcgtggta atccgggcaa acggctgatt aaccgttcgg
    53521 tggagcgggc gctgcatttt cgcaaagagg tccagcggtt gcgggaagag tctgacggtt
    53581 ggtttttcga tatctggcaa ccgccgcagg tggatgaagc cgaatgctgg cccgttgcgc
    53641 taggcgaaca gtggcacggc tttaacgatg cggatgccga tcatatgttt ctcgatccgg
    53701 ttaaagtcac tattttgaca ccggggatgg acgaacaggg caatatgagc gaggagggga
    53761 tcccggcggc gctggtggca aaattcctcg acgaacgtgg gatcgtagta gagaaaaccg
    53821 gcccttataa cctgctgttt ctctttagta ttggtatcga taaaaccaaa gcaatgggat
    53881 tattgcgtgg gttgacggaa tttaaacgct cttacgatct caacctgcgg atcaaaaata
    53941 tgctacccga tctctatgct gaagatcccg atttctaccg caatatgcgt attcaggatc
    54001 tggcgcaagg gatccataag ctgattcgta aacacgatct tcccggtttg atgttgcggg
    54061 cattcgatac tttgccggaa atgatcatga cgccacatca ggcatggcag agacagatta
    54121 aaggtgaagt agaaaccatt gcgctggaac aactggtcgg tagagtatcg gcaaatatga
    54181 tcctgcctta tccaccgggc gtaccgctgt tgatgccagg agaaatgctg accaaagaga
    54241 gccgcacggt actcgatttt ctactgatgc tttgttccgt agggcaacat taccccggtt
    54301 ttgaaacgga tattcacggc gcgaaacagg acgaagacgg cgtttaccgc gtacgagtcc
    54361 taaaaatggc gggataactt gccagagcgg cttccgggcg agtaacgtgc tgttaacaaa
    54421 taaaggagac gttatgctgg gtttaaaaca ggttcaccat attgcgatta ttgcgacgga
    54481 ttatgcggtg agcaaagctt tctactgcga tattcttggt ttcacgctgc aaagcgaagt
    54541 ctatcgcgaa gcgcgcgact cgtggaaagg ggatttggcg cttaatgggc aatatgtgat
    54601 tgagcttttc tcttttccgt tcccgccgga gcgccccagc cgaccggaag cttgcggcct
    54661 gcgtcatctg gcttttagcg ttgatgacat cgatgcggca gtggcgcacc ttgaaagcca
    54721 taacgtgaag tgtgaagcca tccgtgtcga tccatacacg caaaaacgct tcaccttctt
    54781 taacgatccg gacgggctgc cgttggaact gtatgagcag taaggcttgt catcgccgca
    54841 tttgcccggt aacgtgccgg gcattgctac tgtaaaatcg caccatcatg acactcacgc
    54901 tcaatagaca acttctcacc tcacgccaga ttctggtggc ctttagcggc gggcttgact
    54961 ccaccgttct gctgcatcag ttggtgcagt ggcggacgga aaatccgggc gtcactctgc
    55021 gcgctattca tgtgcatcac ggtttaagtg ccaatgccga tgcctgggtt acgcattgcg
    55081 aaaacgtctg ccaacagtgg caggtgccgc tggtggtcga acgcgtgcaa cttgcgcaag
    55141 aaggactggg cattgaggcc caggcgcggc aggcgcgtta tcaggcattt gcccgcacct
    55201 tgttgcccgg tgaagtgctg gtcaccgcgc aacatctcga cgatcaatgt gaaacttttc
    55261 tgctggcgct aaaacgcggt agcggccctg ccgggctttc ggctatggcg gaagtttcgg
    55321 agtttgccgg aacacggctt atccgcccgt tgctcgcccg cacgcgggga gaactggagc
    55381 agtgggcatt ggcgcatggt ttacgctgga ttgaagacga aagtaatcag gacgactcat
    55441 acgatcgtaa ctttctgcgc ctgcgcgtag tgccgttatt gcagcagcgt tggccgcatt
    55501 ttgcggaagc aacggcccgc agcgccgcac tttgtgctga acaagagagt ctgttggatg
    55561 aactgctggc ggatgattta gcacactgcc aaacgtcgca ggggacgctg cagattgcgc
    55621 caatgctggc gatgagtgat gcccgccgcg cggcgattat ccgtcgctgg ctggcagggc
    55681 agaatgcacc aatgccttcc cgcgacgcgc tggtgcggat ctggcaggaa gtggcgctgg
    55741 cgcgggaaga tgcctccccc tgtttacgct tgggcgcgtt tgaaatccgc cgctatcagt
    55801 cgcaactgtg gtggattaaa tccgtcaccg ggcaaagtga aaccattgtg ccgtggcaga
    55861 cgtggctaca accgctggaa ttaccggcgg ggctgggaag cgtacagctt actgcgggag
    55921 gcgatattcg ccctccgcgt gcagacgaag cggtcagcgt gcgtttcaaa gcggcaggat
    55981 tgctgcatat tgtcgggcgc aacggcggac gtaagctgaa gaaaatctgg caagagttag
    56041 gcgtgccgcc gtggctacgt gacaccacgc cactgctgtt ttatggcgaa acgctgattg
    56101 cggcggcagg ggtatttgtg acgcaagaag gtgtggctga aggtgagaat ggcgtaagtt
    56161 ttgtctggca gaaaacgctt agttaagtga aagccggata agacgcatca aacgtcgcat
    56221 ccggcgaaag tcaatcagga ctcgctcacc accaccgtac cgatttccgg gtggctaaag
    56281 ctggtaattt tatccagacg tagctcacgg gtttcgccag cggcctcgac gaccagatac
    56341 tccacatttt tgcgggagac taaatcgctg gcttttgcct gcaatttttc gccatctttc
    56401 agctcaagtg tcagcattaa atgatgctgg caggcgagct caagattatc gtaatcatca
    56461 caattgattg gttgatacgt atcattcatt gacataatcg ctcaccagta agtttgccgc
    56521 agcgtatgct gctttttccc tgacagcctc agaaagggcg tcgtcggcag ccatttcatt
    56581 cagcactttc aaaacgcagc ccagcgcgtc cggaacgtat cctaagtctc cgctggcgat
    56641 ttccgcgtac cgcttgcgta ttaactcaca atatttttcc acatgccctc ctgtcagcac
    56701 tctgacttaa ccgtggatgc aagtctaagc ctacgaagat aaactctgtt tcgcaaggtg
    56761 actataccac actcatgtct gcaatatcag cgccgcaact gcacgtattc cgttacaatg
    56821 gcctcctgat tcgaaaggag ttttcttatg gcgcttaaag cgacaattta taaagcgacg
    56881 gttaatgtgg ccgatctcga ccgcaaccag tttctcgatg cctctctgac gctggcgcgc
    56941 cacccttcag aaacccagga gcgtatgatg ctgcgcttgc tggcgtggct gaaatatgct
    57001 gatgaacgtc tgcaatttac ccgtggtttg tgtgccgatg atgagccgga agcgtggctg
    57061 cgtaacgatc acctgggcat tgatttgtgg attgagctgg ggctgccgga tgagcggcgg
    57121 attaagaaag cctgcaccca ggccgcagaa gtggcgctgt ttacctataa tagtcgggcg
    57181 gcgcaaatct ggtggcagca aaatcagagc aaatgtgtgc agtttgccaa tctttccgtc
    57241 tggtatctgg acgatgaaca actggcgaaa gtaagcgcct ttgccgatcg taccatgacg
    57301 ctgcaggcaa cgattcagga tggcgtgatc tggttatcgg acgataagaa taatctggaa
    57361 gttaacttaa ccgcctggca acaaccttca tgattgtgat ttcccgacat gttgctattc
    57421 ccgatggtga gcttgagatc accgccattc gtgcgcaggg cgcgggcggg cagcatgtta
    57481 ataagacctc aacggccatt catctgcgtt ttgacattcg ggcgtccagc ctgccagagt
    57541 attacaaaga gcgtctactc gccgccagcc atcatttgat cagcagtgat ggcgtgattg
    57601 tcattaaggc acaggaatac cgcagtcagg aactgaaccg cgaagccgcg ctagcccggc
    57661 tggtggcagt gattaaagat ttaacaacag aacaaaaagc ccgacgaccc acgcggccca
    57721 cccgtgcatc gaaagagcgc aggctggcat cgaaagcaca aaaatcaagc gtgaaggcga
    57781 tgcgcggcaa agtacgcagc ggtcgggaat aaaaagaagg aatagatggt gaaaaaagcg
    57841 atagtgacag cgatggctgt aatcagcctc tttactctga tgggatgtaa taaccgggcc
    57901 gaagtcgata cgctttctcc agcacaggct gccgaactga aaccgatgcc gcaaagttgg
    57961 cgcggcgtgc tgccgtgtgc tgattgcgaa ggaatcgaaa cctctctgtt cctcgaaaaa
    58021 gacggaacat gggtgatgaa tgagcgttat ctcggtgctc gtgaagaacc ttcctccttc
    58081 gcttcctacg gtacatgggc gcgaaccgct gacaagctgg tattaaccga cagcaaaggt
    58141 gaaaagtcat attatcgggc gaaaggcgat gcgctggaga tgctcgatcg tgaaggcaat
    58201 ccgattgaat cgcagttcaa ctatacgctg gaaccggcac aatctagttt acctatgacg
    58261 ccgatgaccc tgcgaggcat gtatttttat atggctgatg cggcgacctt cactgattgc
    58321 gcgaccggaa aacgtttcat ggtagcgaat aacgcagagc tggagcgtag ctacctggct
    58381 gcgcgcggtc acagtgaaaa accggtgtta ctgtcagtag aaggtcactt tacgcttgag
    58441 gctaatccgg atactggtgc gccaaccaaa gtattagcgc ccgatacggc gggtaaattt
    58501 tacccgaata aggattgcag tagtttgggg cagtaaacct gcttaaggca gaaacaaacg
    58561 caa
//
LOCUS       DAZGLJ010000006        52885 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000006 DAZGLJ010000000
VERSION     DAZGLJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.006"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..821
                     /gene="ynaI"
                     /locus_tag="ACHK49_000332"
     CDS             <1..821
                     /gene="ynaI"
                     /locus_tag="ACHK49_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO9261031.1"
                     /translation="FITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGRDITSA
                     RIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIML
                     YFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGR
                     MTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIM
                     VYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR"
     gene            complement(872..2485)
                     /gene="mppA"
                     /locus_tag="ACHK49_000333"
     CDS             complement(872..2485)
                     /gene="mppA"
                     /locus_tag="ACHK49_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO9261032.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASMENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2822..3721)
                     /gene="pgrR"
                     /locus_tag="ACHK49_000334"
     CDS             complement(2822..3721)
                     /gene="pgrR"
                     /locus_tag="ACHK49_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO9261033.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGHHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            3859..4779
                     /locus_tag="ACHK49_000335"
     CDS             3859..4779
                     /locus_tag="ACHK49_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9261034.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVSYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSIFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            4779..5711
                     /locus_tag="ACHK49_000336"
     CDS             4779..5711
                     /locus_tag="ACHK49_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFO9261035.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQKALEKVIPFFNTHLG"
     gene            5729..6367
                     /locus_tag="ACHK49_000337"
     CDS             5729..6367
                     /locus_tag="ACHK49_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9261036.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVSALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
     gene            6676..7404
                     /gene="mpaA"
                     /locus_tag="ACHK49_000338"
     CDS             6676..7404
                     /gene="mpaA"
                     /locus_tag="ACHK49_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO9261037.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPATAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(7379..8344)
                     /gene="ycjG"
                     /locus_tag="ACHK49_000339"
     CDS             complement(7379..8344)
                     /gene="ycjG"
                     /locus_tag="ACHK49_000339"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO9261038.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVTTAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFVDLDGPTWLAVDVEPALQFTTGELHL"
     gene            8463..8969
                     /gene="tpx"
                     /locus_tag="ACHK49_000340"
     CDS             8463..8969
                     /gene="tpx"
                     /locus_tag="ACHK49_000340"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO9261039.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(9013..10554)
                     /gene="tyrR"
                     /locus_tag="ACHK49_000341"
     CDS             complement(9013..10554)
                     /gene="tyrR"
                     /locus_tag="ACHK49_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO9261040.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(10702..11763)
                     /gene="ycjF"
                     /locus_tag="ACHK49_000342"
     CDS             complement(10702..11763)
                     /gene="ycjF"
                     /locus_tag="ACHK49_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO9261041.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(11760..13157)
                     /gene="ycjX"
                     /locus_tag="ACHK49_000343"
     CDS             complement(11760..13157)
                     /gene="ycjX"
                     /locus_tag="ACHK49_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO9261042.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            13312..14310
                     /gene="ycjW"
                     /locus_tag="ACHK49_000344"
     CDS             13312..14310
                     /gene="ycjW"
                     /locus_tag="ACHK49_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9261043.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKAEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(14421..15326)
                     /gene="ompG"
                     /locus_tag="ACHK49_000345"
     CDS             complement(14421..15326)
                     /gene="ompG"
                     /locus_tag="ACHK49_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO9261044.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(15371..16453)
                     /locus_tag="ACHK49_000346"
     CDS             complement(15371..16453)
                     /locus_tag="ACHK49_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9261045.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(16467..17126)
                     /gene="pgmB"
                     /locus_tag="ACHK49_000347"
     CDS             complement(16467..17126)
                     /gene="pgmB"
                     /locus_tag="ACHK49_000347"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO9261046.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(17123..19390)
                     /gene="ycjT"
                     /locus_tag="ACHK49_000348"
     CDS             complement(17123..19390)
                     /gene="ycjT"
                     /locus_tag="ACHK49_000348"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO9261047.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(19387..20442)
                     /gene="ycjS"
                     /locus_tag="ACHK49_000349"
     CDS             complement(19387..20442)
                     /gene="ycjS"
                     /locus_tag="ACHK49_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO9261048.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(20452..21240)
                     /gene="ycjR"
                     /locus_tag="ACHK49_000350"
     CDS             complement(20452..21240)
                     /gene="ycjR"
                     /locus_tag="ACHK49_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO9261049.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLHVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEEPAQAYRDSLAWLRTC"
     gene            complement(21258..22310)
                     /gene="ycjQ"
                     /locus_tag="ACHK49_000351"
     CDS             complement(21258..22310)
                     /gene="ycjQ"
                     /locus_tag="ACHK49_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO9261050.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNLIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFAKSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(22341..23183)
                     /gene="ycjP"
                     /locus_tag="ACHK49_000352"
     CDS             complement(22341..23183)
                     /gene="ycjP"
                     /locus_tag="ACHK49_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO9261051.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(23170..24051)
                     /gene="ycjO"
                     /locus_tag="ACHK49_000353"
     CDS             complement(23170..24051)
                     /gene="ycjO"
                     /locus_tag="ACHK49_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO9261052.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(24072..25364)
                     /gene="ycjN"
                     /locus_tag="ACHK49_000354"
     CDS             complement(24072..25364)
                     /gene="ycjN"
                     /locus_tag="ACHK49_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO9261053.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(25378..27057)
                     /gene="ycjM"
                     /locus_tag="ACHK49_000355"
                     /pseudo
     CDS             complement(25378..27057)
                     /gene="ycjM"
                     /locus_tag="ACHK49_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(27269..27583)
                     /gene="pspE"
                     /locus_tag="ACHK49_000356"
     CDS             complement(27269..27583)
                     /gene="pspE"
                     /locus_tag="ACHK49_000356"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO9261054.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(27658..27879)
                     /gene="pspD"
                     /locus_tag="ACHK49_000357"
     CDS             complement(27658..27879)
                     /gene="pspD"
                     /locus_tag="ACHK49_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO9261055.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(27888..28247)
                     /gene="pspC"
                     /locus_tag="ACHK49_000358"
     CDS             complement(27888..28247)
                     /gene="pspC"
                     /locus_tag="ACHK49_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO9261056.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(28247..28471)
                     /gene="pspB"
                     /locus_tag="ACHK49_000359"
     CDS             complement(28247..28471)
                     /gene="pspB"
                     /locus_tag="ACHK49_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO9261057.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(28525..29193)
                     /gene="pspA"
                     /locus_tag="ACHK49_000360"
     CDS             complement(28525..29193)
                     /gene="pspA"
                     /locus_tag="ACHK49_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO9261058.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            29360..30337
                     /gene="pspF"
                     /locus_tag="ACHK49_000361"
     CDS             29360..30337
                     /gene="pspF"
                     /locus_tag="ACHK49_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO9261059.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(30457..31722)
                     /gene="puuE"
                     /locus_tag="ACHK49_000362"
     CDS             complement(30457..31722)
                     /gene="puuE"
                     /locus_tag="ACHK49_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO9261060.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(31760..33040)
                     /gene="puuB"
                     /locus_tag="ACHK49_000363"
     CDS             complement(31760..33040)
                     /gene="puuB"
                     /locus_tag="ACHK49_000363"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO9261061.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(33042..34529)
                     /gene="puuC"
                     /locus_tag="ACHK49_000364"
     CDS             complement(33042..34529)
                     /gene="puuC"
                     /locus_tag="ACHK49_000364"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO9261062.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLTDLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGESNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(34804..35361)
                     /gene="puuR"
                     /locus_tag="ACHK49_000365"
     CDS             complement(34804..35361)
                     /gene="puuR"
                     /locus_tag="ACHK49_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO9261063.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(35388..36152)
                     /gene="puuD"
                     /locus_tag="ACHK49_000366"
     CDS             complement(35388..36152)
                     /gene="puuD"
                     /locus_tag="ACHK49_000366"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO9261064.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            36364..37782
                     /gene="puuA"
                     /locus_tag="ACHK49_000367"
     CDS             36364..37782
                     /gene="puuA"
                     /locus_tag="ACHK49_000367"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO9261065.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            37931..38095
                     /locus_tag="ACHK49_000368"
     CDS             37931..38095
                     /locus_tag="ACHK49_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9261066.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            38085..39470
                     /gene="puuP"
                     /locus_tag="ACHK49_000369"
     CDS             38085..39470
                     /gene="puuP"
                     /locus_tag="ACHK49_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO9261067.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALFVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            39604..39849
                     /gene="ymjA"
                     /locus_tag="ACHK49_000370"
     CDS             39604..39849
                     /gene="ymjA"
                     /locus_tag="ACHK49_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFO9261068.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            40162..41805
                     /gene="sapA"
                     /locus_tag="ACHK49_000371"
     CDS             40162..41805
                     /gene="sapA"
                     /locus_tag="ACHK49_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO9261069.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            41802..42767
                     /gene="sapB"
                     /locus_tag="ACHK49_000372"
     CDS             41802..42767
                     /gene="sapB"
                     /locus_tag="ACHK49_000372"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO9261070.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            42754..43644
                     /gene="sapC"
                     /locus_tag="ACHK49_000373"
     CDS             42754..43644
                     /gene="sapC"
                     /locus_tag="ACHK49_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO9261071.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            43644..44636
                     /gene="sapD"
                     /locus_tag="ACHK49_000374"
     CDS             43644..44636
                     /gene="sapD"
                     /locus_tag="ACHK49_000374"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO9261072.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            44638..45444
                     /gene="sapF"
                     /locus_tag="ACHK49_000375"
     CDS             44638..45444
                     /gene="sapF"
                     /locus_tag="ACHK49_000375"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO9261073.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            45512..45865
                     /gene="ycjD"
                     /locus_tag="ACHK49_000376"
     CDS             45512..45865
                     /gene="ycjD"
                     /locus_tag="ACHK49_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFO9261074.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            46233..47021
                     /gene="fabI"
                     /locus_tag="ACHK49_000377"
     CDS             46233..47021
                     /gene="fabI"
                     /locus_tag="ACHK49_000377"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO9261075.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            47166..48293
                     /gene="yciW"
                     /locus_tag="ACHK49_000378"
     CDS             47166..48293
                     /gene="yciW"
                     /locus_tag="ACHK49_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO9261076.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            48361..50295
                     /gene="rnb"
                     /locus_tag="ACHK49_000379"
     CDS             48361..50295
                     /gene="rnb"
                     /locus_tag="ACHK49_000379"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO9261077.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATAMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(50373..50477)
                     /locus_tag="ACHK49_000380"
     CDS             complement(50373..50477)
                     /locus_tag="ACHK49_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9261078.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            50531..52516
                     /gene="pdeR"
                     /locus_tag="ACHK49_000381"
     CDS             50531..52516
                     /gene="pdeR"
                     /locus_tag="ACHK49_000381"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO9261079.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWREKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(52498..52626)
                     /gene="ymiD"
                     /locus_tag="ACHK49_000382"
     CDS             complement(52498..52626)
                     /gene="ymiD"
                     /locus_tag="ACHK49_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO9261080.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            52664..52837
                     /gene="yciZ"
                     /locus_tag="ACHK49_000383"
     CDS             52664..52837
                     /gene="yciZ"
                     /locus_tag="ACHK49_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO9261081.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
BASE COUNT        12634 a        13909 c        13189 g        13153 t
ORIGIN      
        1 aatttatcac cccaggcgtt atcgatttta tctgtacgtc cctgattgcg gttattctga
       61 cgattaagct atttttgctg attaatcagt ttgaaaaaca gcagattaaa aaaggtcgcg
      121 atatcaccag tgcgcggatt atgtcgcgca ttatcaaaat caccattatt gtggtgcttg
      181 ttctgcttta tggcgaacat ttcggcatga gcctttctgg cttgctgacc tttggtggta
      241 ttggtggtct ggctgtcggt atggccggta aagatattct gagtaatttc ttttccggga
      301 tcatgctcta tttcgaccgt cctttcagta ttggcgactg gatccgttca ccggacagaa
      361 atatcgaagg tacagtagcg gaaattggct ggcgaattac caaaattacg acctttgata
      421 atcgtccatt gtacgtaccg aactcgctgt tttcgtcgat cagcgtagaa aacccaggac
      481 gaatgaccaa ccgccgcatt accacgacca ttggtttacg ttatgaggat gcggcaaaag
      541 tgggcgttat tgtcgaagcc gtacgtgaga tgctgaaaaa tcacccggcc atcgaccagc
      601 gacaaacctt actggtttat ttcaaccagt ttgccgactc gtcattgaat attatggttt
      661 attgctttac caaaaccacg gtatgggctg aatggcttgc tgcacagcaa gacgtttatt
      721 tgaagattat cgatattgtg cagtcacacg gcgcggactt tgccttcccc agccagacgc
      781 tgtatatgga taacattaca ccgccggaac aggggcgcta aatcagacat cactggcgtt
      841 atccacaagg gtaacgccag ttcccatcac atcaatgctt cacaatatac atagtccggc
      901 tgtacgcaac atcttccggg ttattaatgg gataaccttt cagccacggc ttgattaatc
      961 gtccattggt atattgataa attggtgcaa tcggtgcttg ctccatgagg attttttctg
     1021 ccgcgttgta atcggcatta cgcgctttaa cggtattttc catcgatgcc tgggccagaa
     1081 ctttgtcgta tgccggattg ttaaagcgtg aaatatttcc tgaatgcgtt gacgttaata
     1141 atgtcaggaa agtggagggt tcattataat cccccaccca tgaggcgcgg ataacatcaa
     1201 aattgccggt gttacggcta tcgatatagg ttttccattc ctgattttgc aatttaacat
     1261 ctacgccaag gttctttttc cacatcgatg ctacagcaat cgcaattttt tgatggtttt
     1321 ctgaagtgtt atacaaaagc gtcagcttca gcggtttttg cggaccataa ccagctgcgc
     1381 tcaacaaagt ttttgcctgc gcattcagtt cttcctgact catttgttca aacggcgaag
     1441 gttccggcgt aaatccggcg gtaacatctg gtgtaaaatg ccacgctggc ttttcgcccg
     1501 ttcccaacac tttttcggtc atcaggcggc gatctatcgt catacttaat gccaggcgaa
     1561 cgcgctgatc tgccgtcggc cctttttgcg tgttaaacgc ataataatag gtcccgagct
     1621 gtggcggcgt ataaacctgc cccggaatat ccttcaacag cttctgatac atatttttcg
     1681 ggaaggattc ggtgatatca atatcccccg cgaggtaacg cttagtggct gcggattcct
     1741 gattaattgg caggaaggtc actttttgca gtaccgtttt ggcgttatcc caataatggg
     1801 tattcggtac cacgaccagt ttttcattga ctacgcgatc tttaagaaca taagcgccat
     1861 tgccgatcag atttccgggt ttcgtccact ctttaccgct ttctacgttg gctttttgca
     1921 ccgggaagaa ggcaaagtta gcggttaaat tcacaaacca cggcaacggt ttatcaagct
     1981 gaattttcaa agtatgggca tcaactgcgg tgacgccaag ctggtctggc gtagctttac
     2041 catcaataat cgcctgtgcg ttgttgattc ccgccagcgc ggcaaaccat gcaaatggcg
     2101 atagcgtttt cgggtccacc agacgttgcc agctgtagac aaaatcttgc gccgttaccg
     2161 gtgtgccatc cgcccatttt gcgttatcgc gcagggtaaa agtccagata cggttgtcat
     2221 tacttttcca ctgagtcgca acgccgggga caatctcccc tttctcgttc tgattcacca
     2281 gtccttcaaa cagatcgcga atgacctgaa tctctggcag gcccacggct ttagcgggat
     2341 ccagcgacgc aggctcatct ttaatgtggc gcaccagctc ctgcttctct gccagtactg
     2401 tgccgctcgg aacttctgca gcatacgaaa gagaaatgct gctgaccaac agcgcacaac
     2461 acgtgactga aacagagtgc ttcataagtt tccctctaac cgacattaaa attgatgcgt
     2521 aattatttgt ttcgccttaa agaaatgcaa acaacttcca tcaaaaaagt gacacaggaa
     2581 gcataaaatg ttttatgtat caatatatta aatcagattt agtgtaaata atcgacgcca
     2641 aaatgcggta aagcacatag aacaccgaac actacgttca ggcttcgcac gttcaaacca
     2701 gttttcggta tcataatcag catctgtcgc cattgtacga agaataaaaa ataaccaaaa
     2761 cgttgaaagg aactgagaat acgttaagag agaaattaaa ttaatgtatc agcaagtagt
     2821 gctaaacagc acctttatac ttcagcctat ctataaataa tgagaatgcc gagccagcat
     2881 gtcgacggtg tggatagtac aggtgatgac cgggtaaatc tggtgtgaat ttatccaaaa
     2941 cacgtatcag ttttttttct ttaatagccc gctcaacctg atcataaggt agatacgcca
     3001 atccatgccc atcaattgca gcatcaatga tcaggtctat cgtattcagt aaaagctgac
     3061 cttccatgcg aacacgaact tcacgtccac cgcgtattaa tctccagcga tttgctgtac
     3121 ccgatgtggg aagatacaaa ttaattgcct gatgatctat taattgtgac actgacgttg
     3181 gaacacttcg gcgagaaaaa taatccggtg agccaacaat agccattggt atatctggcc
     3241 cgattcgaat ggcgatcata tctttatcca tctccccacc cagacggacg cctgcatcaa
     3301 aacgttcaga aacgacatcg gtcaaaccat aatcaatggt gagctgaata tcaatttcag
     3361 gatgcgattt caggaatgtg cgcattgctg gtaacaatat cgtttttgct gcatgttcta
     3421 cagtagtaat acgtattgtc ccggatgggc ggttctgcag ctcgctcagg gatgccaggg
     3481 ctgaatctat gtcatgcaac atagggccaa gaacagacaa aagatgctcg cccgcttcag
     3541 ttggaacaac gctgcgcgtg gttcgcgtca gaagccgcaa tcccaatcgt tcttctatac
     3601 gacgcactat ctggcttaaa gctgactgcg ccatgctcag gcgggctgct gcacgagtga
     3661 agctacgctc ctctgcaacg acgacaaacg ccatcagatc agcgatttct tcacgcttca
     3721 ttcagtatct ccatgcaatc tattaatcat ttaaatgata ggtctaatca tgattttggg
     3781 tctaatcaac ttaatgatca atatctatag tgatatcacc aaccgttaaa acgaaccgac
     3841 aactccatga ggaacggaat gatgaataat aaagtcagct tcactaacag caataatcca
     3901 accatctctt tgtctgcagt gagctatttc cctccaaaat ttgatgaaac ccgtcagtat
     3961 caggcaattg tggtatctca cccaggtggg ggcgttaaag aacaaacggc cggaacctat
     4021 gccaaaaaac tggcggaaaa gggatttgtc actattgctt atgacgcatc ttatcaaggt
     4081 gaaagtggcg gcgagccgcg tcagttagaa aacccataca ttcgtactga agacatcagt
     4141 gccgtgattg attacctgac aacgctttct tatgtcgaca atacccgaat tggtgcgatg
     4201 ggaatttgtg ccggtgcagg atatacagcc aatgctgcaa ttcaggatcg ccgcatcaag
     4261 gccatcggta ccgttagtgc ggtcaatatt ggttcgatat tccgtaatgg ttgggaaaac
     4321 aatgtgaaat ctattgatgc cctgccatac gttgaagcgg gttcaaacgc cagaaccagc
     4381 gatattagca gcggagaata cgccattatg ccactggcac caatgaagga gtccgatgcg
     4441 ccgaacgaag aactgcgtca ggcctgggag tattaccaca cccctcgcgc gcagtatcca
     4501 acagcaccgg gttacgctac tttgcgcagc cttaaccaga ttattaccta tgatgcttac
     4561 catatggcgg aagtgtacct gactcagccg acgcagattg tggcgggcag ccaggcaggg
     4621 agcaaatgga tgagtgacga cctgtacgat cgagcatcaa gccaggataa acgctatcac
     4681 attgttgaag gcgcaaacca tatggatctt tatgacggca aagcctatgt ggctgaagca
     4741 atttcagtat tagctccgtt ctttgaggaa actctgtaat gaaacatatt caaatccgca
     4801 atagtgatat ggcctggcat attgctgcca atattcagtt tccaccaaat tttgatgagt
     4861 caaaacagta tccggcgatt atcagcgtcc atccttttgg cagttgtaag gagcagacgt
     4921 ctggcaatat ctatggcaaa gcattggctg aaaaaggcta tctcgttctc gcttatgacg
     4981 ccagtttcca gggagaatct ggtggggagc ctcgctggat agaagatccg acacagcgcg
     5041 tagaggatat tagccgtgtt atcgattatg cagtaactct gccatatgtt aatgctgaac
     5101 gtattggcgt gctgggagtg tgtgggggtg gtggctatgc cattaatgcc gcgctgacag
     5161 aaaaacgaat taaagcagta gtgagtatta cgggtgttaa tatcgggcgt ctttttcggg
     5221 aaggtttcag caattacgat ccgattggcg cattgaatgc aatggcgtcg caacgaacca
     5281 aagaggctcg tggtggagaa ttacagatca acgaattact tcccgccagc ctggatgcag
     5341 caaaagccca tggactgacc gaacgcgatg tttatgaagc aactgactac tataaaacac
     5401 cacgcgggca acaaccggga ggtgcgacaa aaatgttgtt ctcacatgca cagaaaacgc
     5461 tggcctggga tgccttcgca tttactgaag tgttactgac tcagccggtg atggtagtag
     5521 ttggtgaaaa agtcggtgcc ttcggtgctt atcgtgatgg actggaagtc tatggccggg
     5581 cgatggtttc gcaggatcgt cagttagttt cattacccga tttctcacat tatgaactgt
     5641 atgacaaacc ggaagcagtt cagaaggcgc tggaaaaagt tattcctttt ttcaacaccc
     5701 atctgggata agtttcatga gaaatattat gaaaaatgta ctgattcttg gtgctggcgg
     5761 tcagatagcc cgccatgtga ttaatcaact ggcagataaa caaaccatta aacaaacttt
     5821 atttgcccgt cagccagcaa aaatccacaa accctatcca actaatagca agattattat
     5881 gggagatgta ctgaatcatg cagcactgaa gcaggccatg cagggacagg acgttgtgta
     5941 tgccaacctt acgggggaag atttagatat tcaggcaaat tcagtgatcg ctgcgatgaa
     6001 agcctgtgat gttaaacgtc tgatctttgt tctttccctc ggtatttatg atgaagttcc
     6061 aggaaaattt ggcgaatgga ataatgcagt tattggtgag cccctaaaac cattccgcag
     6121 ggctgcggat gcaattgagg cttctggact ggaatataca attcttcgcc ctgcctggct
     6181 gactgatgaa gatattattg attatgagct tacctcacgc aatgaaccat tcaaaggtac
     6241 gatcgtatca cgtaagagtg tctcagcatt aatcacagat atcatcgata aacctgagaa
     6301 acacattggt gagaatatag gtatcaatca acccgtgact gatggtgaca agccattatt
     6361 catgtaaatc aattacatag ttaccatcgt aatgtactat gtgcagtacg atttattaaa
     6421 cttattaagg tgaactgatc aatccgatgc gattaacatg tgtagaacca gtaagaataa
     6481 aatacgccat gtatataaca ttacgactta catctaagtc agcataattc taatatctgc
     6541 acatcaataa aaatgcaaac aacttccggc aaaaaagtga tacaagatcg atgacatagc
     6601 gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt aatacttcct
     6661 gaaccagaga cgatcatgac cgtaaaccgc ccacgcgccg aacgcggcgc atttccgccc
     6721 ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt tccggccact
     6781 gcagccagtc gcgaaagtgg tttgattctg gctggcactc acggtgatga aaactcttcg
     6841 gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg ccatcatgta
     6901 gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc taatggtgtg
     6961 gatttaaacc gaaactttcc ggcggcgaac tggaaggaag gtgaaacagt ttatcgctgg
     7021 aacagcgccg ctgaagaacg tgatgtcgta ttgctgacag gtgacaaacc tggctctgaa
     7081 cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacccgcctg ggtggtctca
     7141 ttccacgatc cgctggcctg tattgaagat cccagacaca gcgaattagg cgaatggctg
     7201 gcccaggcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacacc tggctctttc
     7261 ggtagttggt gtgcggatct caacctgcat tgcatcaccg cagagtttcc gcccatctcc
     7321 tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg ctggcatcct
     7381 aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc gccagccagg
     7441 tcggtccgtc aagatcgacg aaactgacct gcggcaccag cggtaaagcg gcgctaatgg
     7501 cccgagaggt acacaacatg cagcccagca tcagacgaaa accttgtgca cgcgcttcag
     7561 tcgccagcgc cagcgcttcc gtcagaccac cggttttatc aagcttaatg ttaaccatct
     7621 catagcgccc tttcagcgcc ttcagattgc tacgtgtatg acaactttca tctgcacaaa
     7681 tcggtaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga agcggttgtt
     7741 caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac ccttctgcac
     7801 gccaggattc atttgcgtca acgatcagcg tcgcatcggg cacagctgtg cgaattgcca
     7861 ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc gcgcctgcct
     7921 gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgact accgtctgtg
     7981 cagtggtgac tgtcccggga agcgttatgc cgatcaaatc agccagcgat tgctgctgtt
     8041 ttcgcgcggc cagatcccac aatgcacaat ccagcgcatt acgtgctgcg ccggcaggga
     8101 gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg acgctcataa
     8161 tttgcgccat taccgaggca tcactttccc cataacgcgg atacggcgtg cattcgccgg
     8221 tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca ctgcgacttc
     8281 cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc ttaacggttc
     8341 tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag cgtttgctgt
     8401 gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca ggaaagataa
     8461 tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat tccatcccgc
     8521 aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct gacgtcaccc
     8581 tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt gataccggtg
     8641 tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac aacaccgttg
     8701 tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc gcagaaggtc
     8761 tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa gccttcggtg
     8821 tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg gttattgacg
     8881 aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag ccggattacg
     8941 aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt gcaccatcag
     9001 gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc gcaacttatt
     9061 ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc tgggataatt
     9121 gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt ccagcgaacc
     9181 ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca acaaaatatc
     9241 ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag cgttctttaa
     9301 ctgccgcaca tttcccggcc acgcataacg cgtaagtaca gtattcaggt cagcggccag
     9361 tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct cagttaacgg
     9421 catgatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca cgttcagacg
     9481 ataatagaga tcttcacgga acatgccttt ttgcaccagt tcgaccagat tcttctgcgt
     9541 agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc gacggaaagt
     9601 gccatcatta aggaaacgca gtaatttcgc ctgcatccgt ggtgacattt cccctatttc
     9661 atccaacagc accgaaccac cgttcgcctg ctcaaagaat cctttcttcc cttccggagc
     9721 atgaccaaac agttcactct cgaccgcatc ttccggtata gacgcacagt tcagcgccag
     9781 gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaga gatctttacc
     9841 tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt tctgcgcctg
     9901 ttcgacaaca tgcttcattt tcgggctgac ggcgacaatt tgactgaagg cgctgacgtc
     9961 ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca acatcaccac
    10021 cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg taatctccat
    10081 caggaaattc tgcccattaa taacgacatg ctcgttatgg gaatcttgcg gttcgctttc
    10141 cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt tgcgcaggcg
    10201 atccaatttt tgcgcaaaaa gctgacagct cgccgggttc gccatatcca ctttgctttt
    10261 catatcgaca gagagcacag gttcgggcaa cgcctccagt aacgcgctca acgccagatg
    10321 ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg caatacggcg
    10381 tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat tgaggtagat
    10441 tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca cgagtagatc
    10501 gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac gcatgggaac
    10561 cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat agcaggaagg
    10621 tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg taaagatatc
    10681 cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt gcagcgtttc
    10741 tttcacctga ccgataagct gacgacggaa atccccgagg cgaggtttgt catcgtcaat
    10801 ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtgccg tcagaagtcc
    10861 agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt gcgacatcca
    10921 gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat tcagcaatac
    10981 cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca gcgtggcgat
    11041 gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca acgccagcgg
    11101 gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc gccgcgcctg
    11161 ggcatctaaa actggctgga ccaaatgcgc atacaaactg accacttcac ggtcgttttg
    11221 cgtttcatgg attgaggcat accagcgttg cagcgctgga tgagactgat caatacccgc
    11281 ctgctgcgcc agtttttcgc aaaatgcgcg gcctttgccc gtgccgtggc tgtgcaacaa
    11341 atcgcgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc gccgccactc
    11401 tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac atccacccag
    11461 cgccacccag tcctgagttt gccaggcatt cattgtccac tgtacaccct gcccgacaac
    11521 gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc tgcgtttcgg
    11581 acgtaacgct gcatccatta ccgcttcaac ttgcccctct tcttcaggcg cttcatcgag
    11641 cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg ccctgaattt
    11701 tggattctga tcgacctcca gcggaccgtc gaaatcaata cgtggtttta acggttcggt
    11761 catcgcaatt tatctcctat taaaaattca agcgcggcat caagacgaat atgcggcagc
    11821 ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc ttgcttgtcc
    11881 cagaacgcct gaccgggcaa acgcgcagga acttcaccgg gataaacggt gagtggcgca
    11941 ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac gtcaataatg
    12001 ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat cccttcaaac
    12061 gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat attggcgtgc
    12121 tgatcgatgg tcacatggtc cgctttagtg gcagcaaaca atagcttatc gataaccggc
    12181 gaaaacaaac gccggaacaa ggtacgctgc ccgtagtgga aactttgcat cagctgcgtc
    12241 agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg caggcaatca
    12301 accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc cttcaccacc
    12361 ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg cttatcggcc
    12421 tgcgccagtt tggactcgcc ccaggcatcg acatccggcc acgggaagaa ttgcagcgcg
    12481 ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa gtgcagcccc
    12541 tgctgtttac agtggtggag ataatcggtc cacgcggcgg caatgtctgc cagccggttt
    12601 tcgtcggcag gcgctagcgg gtccagccct tcgcacatca ttcgccattt gaccgaccat
    12661 tcgccgcgct gaccattgag taagcccgtc atctggcgtg accagcttaa atagtcctgc
    12721 gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatctc cagatacagc
    12781 gtggaggtgt ccttgaagtg gcgtagcagc gaatcgttcg atttaaaacg tagcgccagg
    12841 cggatttcac tgacgccgcg cgttggcgtc ggccaggcgg gaggatcgcc atataactgc
    12901 gccagtcctt catcatatgt gaagcgcgga atgccaaagt cacgctgagg aatgcgtttt
    12961 acgcccagca ggcgctcttc acgcaccgcg cttaacagcg gcaagcgtgc tccggcgtga
    13021 atattgagca actgattgac catcgcagtg ataaacgctg ttttgccgct gcggcttagt
    13081 ccggttacgg cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc attaagttca
    13141 tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa aatcattcgt
    13201 aaggatatga atgtgggggc caggccattg attatcaact gcccgtgttg tcatcgacgg
    13261 gcgaactcca taagatgtgc agcattcacg ttcggacgga gttcctgacc tatgtcccct
    13321 actatttatg atattgccag ggttgcaggc gtatcaaaat ccaccgtatc acgcgtgctg
    13381 aataagcaaa ctaatatctc cccggaagcg cgcgaaaaag tgttacgggc cattgaagaa
    13441 ttacagtatc agccaaacaa gctggcccgc gcgctgacct cttcgggttt tgatgccatt
    13501 atggtgattt ctacccgttc gaccaaaact acggcgggta atccgttttt ctctgaagtt
    13561 ttgcatgcca tcaccgccaa agctgaagag gaaggtttcg acgtgatatt gcagacgtcg
    13621 cacaatccgg cagaagactt acaaaaatgt gaaagcaaaa ttaagcagaa aatgattaaa
    13681 ggcattatta tgctaagttc gccagcggat gagtcatttt ttgcccaact tgataaatac
    13741 gatattcctg tcgtggtgat tggcaaagct gaaggtcaat atgcccatgt ttattctgtc
    13801 gataccgata attttggcga cagcattgcg ttgaccgatg cattaattga aagtgggcat
    13861 caaaatattg cctgcctgca tgctccgctt gatgtccatg tttcagtgga tcgggtaaat
    13921 ggttataagc agagcctggc tgcgcataat attgcagtgc gtgatgaatg gattgttgac
    13981 ggcggttata cccatgaaac agcactgcaa gccgcgcggg aattattaag ccagtcaccg
    14041 ttgcctgagg cagtgtttgc tactgacagc ctgaaattaa tgagtattta tcgtgcggca
    14101 gcagagaaaa atattgctat tccgcagcag ttagcggtgg tgggttatag taatgaaacg
    14161 ctgtcgttta ttttaacgcc tgcgccaggc ggtattgata tcccaacaca ggagttaggt
    14221 caacaaagct gtgagttatt attccgctta atttctggaa aaccgtcacc acagaatatt
    14281 accgttgcca cgcatatgac gctgaaataa ataccagcta ccttgcctga atgccggatg
    14341 cgatgctagc gcatcttatc cggcctacat ttatagttcg gataaagtac tcgtgccgca
    14401 tccggcaatt tagcccatta tcagaacgag taatttacgc cgacacctgc ataatggaat
    14461 ttatcgctgt cgccttcgtc gtgatcctgc cactcaaacg cgtattccag cgaaacggaa
    14521 agcccgttct ggaaatcata accgtaaaat aaacctacac ggttaaaatc atggccttca
    14581 cgttcaatat catcctgcca gtcccagtta ctccagcgat ccagcccaat gcgcgtatac
    14641 ggcgtcaccg agtggttgcc gagcgtcagc ggcaaatagg cgcgaatttc ttgcgtagaa
    14701 aactcaccgt tattgcggct gtcgtccata ttaaagccgc gctcgagata atagttcact
    14761 cgcaaggcaa ccgtttcgtt gaaggtatat tgcaggcctg tttccgtttc gactcgggta
    14821 tcagcgtaac cggtcgtgtt cagatcgttg gcaaatttat acatcgacaa ccaaccgttg
    14881 aaacgtaaat cgtcagtcag tttcacatcc cagtctggcg cgattttcca gcgctgcata
    14941 ttcgccgtgt ctttacccgg ttcatcaacg tagtgataac cataattacg gaaaccgccg
    15001 gtcaggccga aactgaaatc atcgttttcg aggaactgat aatgcacctc cagctccggg
    15061 cgatcaaacc acgttccacg tttacccgcg ctataatcta ccggcccttc ctgataatag
    15121 gccagagcaa ttctccacgg cccgttggcg gcattaaaat agactgaagg ctccgccagc
    15181 ccatccatat cttcgccata accctcgacg ttttctattt cgtacatcgc gccgatatta
    15241 aagtgccagt cgttcctttc ctcggcctgt gcgcaggcca ttcccgcaca catcaccagt
    15301 gcggtacagg gtaataactt tttcattatt gttccttgta ttattctggg ggaatgcctt
    15361 ccccctgtat ttagcgaatt gctatttccg tttctgcatc aaagaaatga catttcgtca
    15421 tatcaaaatg aatagtgata ttttctcctg catgataatc atttaacgca ccagcacgga
    15481 ccactaactc gtgcccccca accgtggtgt agagcataaa ttccgcaccg gttaattctg
    15541 ccacgctaat tttggcggaa atgttatttt cctcttgcgc gtccggatga atatcttccg
    15601 gacgtattcc catcacgatg ggcttatgca aacttccctg tgttttcaga accgctaatt
    15661 tctcttcggg aatggttaat ttaagcgttt ccgtaacgaa tttatcgcca tcgatcgtgc
    15721 cacgaataaa attcatcgcc ggtgatccaa taaatccggc aacaaacata ttcgctggtt
    15781 ggttataaac cgttttcggc gcaccaactt gctgaacaat cccatccttc ataatcacaa
    15841 tccgcgtcgc catggtcatc gcttcggtct gatcgtgggt cacgtagatc atggtggtat
    15901 tcagcttctg gtgtagcttg ctgatctctg cgcgcatttg cacgcgcagc ttggcatcaa
    15961 ggttagagag cggttcatcc attaaaaaca cgcccgcttc gcgcacaatc gcccgcccga
    16021 gcgccacacg ctggcgttgc ccgccggaaa gcgcgcccgg cttgcgtttc aggtactcac
    16081 gcaggccgag aatttgcgcc gcccagttca cccgctcatc aatcacctct ttggcgattt
    16141 tttgcatctt caggccaaac gccatgttgt cgtaaaccgt catatgcgga tacaacgcgt
    16201 agttctggaa caccatcgct atattgcgtg ctttggctgg aacgtcattc attcgtttgc
    16261 cgtcgatcaa cagatcgccg ccgctgatct cctcaagccc ggcaatcatg cgcagggtgg
    16321 tcgacttacc gcagcccgac gggccgacaa acacgatgaa ctctttatcg gcaatttcca
    16381 ggttgaagtc cttcaccaca tgcacctggt tatcgtagat tttttgaata tgttgtaacg
    16441 aaagctgagc catgttgatt cctttgctat acgttttgcc agaaggccga taaccgcgac
    16501 caggtcagag agtccgttga aggcaacagt aattgcgccc cggttaagcc cgcgccgatc
    16561 cccaccgagc gcataccgct ggcgttaatg gcgtcaatgc ccgcctgcgc atcttcaatg
    16621 ccgatacatg cctgcggcgg cacgcccagc ccggcacagg cggcgagaaa gatttccggg
    16681 tccggtttcg agtttttaag ttgggaagca tccgcgcaga aggtgaaaaa ctcgcgcagc
    16741 tccagcgccg ctaaaatcgt cggcgcattc agggagacag aagccagccc aaccgggatc
    16801 tgctgtgcac ggagatctgc cagcaaagag cgaatgccgg gtagaacagc gctgaccgtc
    16861 aactcgcgca gtgagtggac atagagcaga tttttgcgat acgccagttg cgccctctcc
    16921 tgcgagttaa agtcgccctc tttgcccccg tgttgcagaa tgcgccgcag agactcatcg
    16981 cggctgatcc cttttaggga ttcgttaaac tgcgcatcaa tgctgatgcc aatttcagcg
    17041 gcaatctgct gccacgcctg gaaatgcaga tgcgcggtat cggtgattac accatccaga
    17101 tcgaaaatta ccccttgcag tttcattcat cctcctgatg cgtggtagcg gtcccattga
    17161 agggcaaaat aaaatcaccc aaacagaaaa cagattcttc agccacggat ataagctgac
    17221 cgttcaaacg cagtgaaacg ggcgcagaag ttcgaatcgc aatacgctgc gcgtcaagag
    17281 tgacctgtaa ttcgcagccc tgccagaaca gagggaaaga caactgttgc cactgctcag
    17341 gtaacgccgg attgagatgc aattcaccgt cacgcacgct caccccggca aaaccctgaa
    17401 tcgcccccag ccagatagcg ccagttgcgg cagcatggat accatcatca caactatgcg
    17461 gatcagcacc aagatcgatt tcagtcccct cgcgccagaa ctgataactt tgggtcagca
    17521 ggccacagcg tgcggcaaca atgccgtgga ttgctttact taatgacgag tcgtgaatag
    17581 tgcgcggttc ataaaattgc agattggcaa gacacgatgc cgctgagaac tgctccggca
    17641 gcatgtaatt gagcatcacc acatcagctt gtttgaggat ttgcatctcg ttcacttctg
    17701 cgcgtgaata atccagcaga atggtttgct tccccgccgc cgctttgtat ttcgccagat
    17761 taatcgccgg cttagccata aacgaatcat cctgcggcaa aacgccgtcg ggctgaattt
    17821 ctggcagccg cagttcttta aggaacattt cggcgcgatg gataaacgca tcgtcgctac
    17881 agccaaactg gcgggcaata ctcagcgcct gctggacgtt gtaatacgcc atatagctgg
    17941 tgaaggcgtt attattgaca tgttcggtat attcgtcagg cccaataaca tcatgaattt
    18001 ccagacggtc gttaaccctc accgcgcggc taatccagaa ctttgcagtt tccagaagta
    18061 gcgccatgcc ttcatgcgca atgaaacttt catcccccgt ggtctgccag tattgaataa
    18121 ccgcccaggc gatatcggcc accagatgat gttccgcctg cgccgaggcc actttttgcc
    18181 gcagcccggt gcgaatgtta atggcggcaa attccggcgt ctcttcttcg ccgctgcgcg
    18241 cgctttccca cggaaatagc gcgccctgcc agccgttgcg tcgcgctttc tcctgcgcgc
    18301 ctggcaagtt gtgccagcga taacgcagta aacttcgggc aaccgtcgga tcgctaaaca
    18361 gatgaaacgg taacaaaaat acttctgtat cccagaaaac gtggcctttg tagccttcgc
    18421 cggttaagcc ttttgccgca atgctgctgc gctcgtcgtg ggcaggcgtc atgatgcgca
    18481 gatgataaag cgcataatct aacgcttgct gatcgtgcgc ttcgccgcca tttaccgtga
    18541 tacgacgttt ctgccaccat tgacgccagt tttctgttga tactgcaaga agttggtcgt
    18601 aactctgctg cgcgcacatt tcaagctggc gaagcgacgc gctgccccac tcgtctaaaa
    18661 cggcttgcct gtcatcccgc cagtcgatcc acaccagttt ttgcaacgtc actgtctcgc
    18721 ctgcatgaag ctgcgcactg gtatgttgct gcaaacggcg ctctttggcg gtatagcatt
    18781 gctgcacatc accgctcacc atacagcaac agctgatggc cacatcactg cgtccatcct
    18841 gggtggtgta gatcccctgc atcagatgct gaccaaacac ccgcacctgg gtttcgtcga
    18901 gatgttggcg accgtggttg gtttgcgtgg cgtcgatacc tgttgaaatc agcactgacg
    18961 cgtcggcgtc cagtggcgta atagtgattt ccagcacaat gagcggcagt tggtctgccg
    19021 aaacaaagcg acggctggcg atggtgtaac ctgtgccgtt gctggtacgc cagacaacgc
    19081 tacggcgtaa ttctccactg gcaaagtcaa gttcacgctg ccaggcttcg cgggataacg
    19141 agaaaacctc accatttatg gcaatctcca tccccaagat atcaggcagg ttcaccagtt
    19201 cgttgatttc accttttccc gcccgatgat acagccccgc cagatacatc cctcgcgtct
    19261 ggcgcgtgta atcttcttca tggctggcgc gaagcccaag atagccgttc ccctgcgcca
    19321 tcagcgatgc atacttgttc agggtatgct ggctgaaatg gggttctgat aacgttactg
    19381 gcctggtcat aattccacac gcgtccctgt ttctgcggat tgatacaacg ccgccaccag
    19441 ttgctggatg atgtacccct gctcggcgtc ggctatcatc acgggcttgc cctgtacgtg
    19501 attgataaag gcttccatgc tgcgcaaatg gcggttgtcg tctgccagtt cccgttgcat
    19561 cagcgtcatt aattcaccgt tgttatcggt gtagatatgt gctggaaaca gcgtcgcacc
    19621 ggctttatca ccacaaaagc tgacgttcat aatcgactgt tcgcggatgt tgagtgcaaa
    19681 tgacgtttcc agccacagaa tgccgccgtt atgaaattca atggtgccaa ataacgaatc
    19741 ttcgacgctg taagttgccg gatcccactc gccaaattga ccgcagctct tttgcgtgcc
    19801 gatcttttga aagctatgcg cattcacgct tttcaccgcc ggaaagccca gcacatacat
    19861 cgccgcatcc agcatatgaa tgccgatgtc gataagcggg ccaccaccct gcagttcttt
    19921 attggtaaag acaccccagc cgggaacgcc gcagcgacgc agggcgcggg cggtggtaac
    19981 gtaaatctct cccaaaacgc cgttggtcac ctgttcacgc agctgttgcg tatcgagcgc
    20041 aaaacggtgg tgaaagtcgt aggccagcac cttgcccagt ttgcgcgcgg tatcgcacat
    20101 ttcccgcgcc tgttctggcg tcatggcggg cggtttttcg cacatcacat ggcagcccgc
    20161 ttccagtgcc atcagggtat gttcgtaatg aaaacggtta ggtgagcaga cgctaaccac
    20221 atcaggtttc accgccagca gcatggcctg cggatcgtcc cacacggatg cattaccgta
    20281 tttatctgcc agcgcctgcg cctgggaaag gcggctgtca cagacagcca ccagttccag
    20341 atcgttgcgg gtgcagtagt acgaagcatg aaccttatcc gccacctggc ctgcgcctat
    20401 tatcgcgacc cgcagcggag agcttgtcat tgcacttttc acaaagacct cttagcaggt
    20461 acgcaaccag gccaacgaat cacggtacgc ctgggcaggc tcttccgccc ggatacgccc
    20521 ttcgtacact acatagccct gatagttatc cgcgcgcagc tgttcaaaca gcgcgtggaa
    20581 atccagggtg ccgctgcccg gctggtagcg atggttatcc gcaatatgca catgaccgag
    20641 caggtcgcgg ttgtcatgca gcgcctgcgc caggttatct tcttcgatat tcatgtgata
    20701 gaaatcgccg ataatctgta catgtttaag atcgttttcg acgatgtaac ggcgggcatc
    20761 ggcgagggtg ttgatcatat gatcctgata gcggtttaac ggttcgagat acaccacggt
    20821 tccggtacgc gcggcgacct gttccagtac gtgcagggaa tcactcacca ttttgcggtc
    20881 gccgtccagg ctacgcggcg aggtcatcgg cggtaagcgg aaggtaaaca tgccccacgc
    20941 tgccggaacg acgatacctt taccccccac ttctgccagc gcttcgagaa tgcgttcgat
    21001 ctgctttaag ccattaagac gacgctcttc gataaagtcg ccaatccaac cgtcatagcc
    21061 accgcaggcg gtggtcaccg gtaaaccggt ttctttgatt gctgctttga cttcttcgag
    21121 attgttgacc agcagtttgc cgtcaatctc aaaaccatcg aagcccatct ctttgatata
    21181 acgaaatttc tccagaatgt tttccggaaa gaatgcctga ttctgtgtgc ctattttcat
    21241 tttttcattc ctttgaatta aaacgtaacg cccattttga tgctctgttc cggatgctga
    21301 tcgacatact gcatgtagct ttccgggctc ttggcaaagg tcactaccgg gtcgattaaa
    21361 tcttcgcaat tgagataacc gttcatcagc agttcccagc aggtttcttc aatacgctta
    21421 cggctccagc gcggataatc cgggttcggt tcgctgcacg cacgggagaa aacaattttg
    21481 gcgttattga aatgcgcttc gcgtccgagg ttaaaacctt cagcaaatgg tttggcaaac
    21541 gcgacataag agatggtgcc gccataagca aggccacgca gcgccgattg cagcgcgtcg
    21601 gcgtaaccgc tggtttcgat aatcacatcg gcaccctgct tgccggtcag cgttttgatc
    21661 tcttttccta cgtcagtgcc aatgaggtta aggcagaaat cagcaccgtg gcgacgggca
    21721 atatcacagc gataggcgat aggatcgacg ccaatcacca ccgacgcgcc agcgcgtttc
    21781 gccagttgga tggctatttg accaatcgcg ccaagcccta ccaccaccac aaaatcaccc
    21841 acgcgcacgt tggcatcgcg cacgccgctc atggcaaact gcgccgggtc gtaacaaacg
    21901 gcgtttttcc acgagctgcc ttccggcatt ttgcgcagct tgtagttatt caccgcgtta
    21961 atgatgaccg tctcggagag cgggccgtag ccgcatacgc tgtcgcccac cgcgtagtcg
    22021 gtaacgtcgc tgccgcactc gataatgtcg ccaaccacca tgttgccaag ctggaatttg
    22081 ccaaactcaa tgccgcgcgg cgcatctgcc ggacgcggag tgaacatctg ccattcgccg
    22141 ttaaagtctt catcgataaa cgggctggcg gcgcggaagt cgaccacttc cgttccgtgt
    22201 ttcggtgcgc cgaaacgcac gcggattttc acttcattgg ctaaaatcgc ccgatcttca
    22261 tactcaacca gcgcagcaac acgcggtgct gtggctacta actttttcat ttaaaactcc
    22321 ttgtaaagaa actggccgct ttagcccttc acgccaccgg cggtcaaacc actcttaata
    22381 aaacgttccg aaagcgcata cataatcacc accggcaatg cggtcaccag tgaggccgcc
    22441 atcatccgtc cccagatgta atctggcgta ctgaacagcg cgttcaggcc caccggtaag
    22501 gtgaaattgc tggcgctgga gaggaaaatc gacgcaaaca gatagtcgtt ccacgccacc
    22561 ataaagcagt agacgaacac ggatatcagc ccggacatcg ccaacggcac ggtgatgcgg
    22621 aagataattt gcagacggtt gaggccatcc atcatcgccg cttcttcgat ctcatcgggg
    22681 atggtgtcga agtagctttt cagcataaac acggcggtgg gcagagtttg cgtcaccatg
    22741 gtgatgatca gcgccatctc ggtgtcgtaa atgcccagcg cggtgatgat tttgaacagc
    22801 ggcaccacca gcaaaatgcc agagaacata tacaccgtgt aaaagctggc attgatggtc
    22861 atccgacctt taaagcgcag gcgggaaagc gcgtaagccc caagaatgcc gaggaatacc
    22921 gccaccacgg atgaaaccac cgacaccacc agactgttac ggaagtagtc gacaaacggg
    22981 aaaatcatcg ggttaaaaat gtcgacgtaa tgctccagcg tccactgctg cggcagcagc
    23041 gtaggatgca gtgagatcgc ctctttcgcg cccttgaacg aggtcatcag catcacaaaa
    23101 aatggaaaca gcgtgatgat gagaaacagc gccagcccgc agtaaaaacc gatgcgactg
    23161 agtgtgcgtt tatttgttgc cattgaggtt cacccgtttc ctggtcagca gaatgacagc
    23221 gaaaataatg atgaagagca ccaccgagat cgccgccgct tttcctaaat cattaaaggc
    23281 aaaggcggtt ttgtagagat atacaccgag aatatcgacc ttggtcgtca gcaaataaac
    23341 atccgcgaac atgtagaaca tccagatggt gcgcagggtc accaccgtcg ccaggaccgg
    23401 cataatcgcg ggcagcgtga cgatacgaaa ccgttgccag gcattagcgc catccatctc
    23461 tgccgcttcg tacagcgatt tgtcgatggt ctgcaaaatc gccagaaacg agataaaggc
    23521 atacgggaag tagcgccaga tggcgaacag caccaccagc acaaagctac tgcccggatt
    23581 atcgaaccac agcggtgcct gctcatagag atgcagaaga tcgacgccga ggtagttcac
    23641 aatgccgtag ccgttgttga acatgtattt ccaggcaaac accagcgaaa tggacggcgt
    23701 tacgtaggag aggatcacca gcgaacgcgc ggttttgcgc agacggaact cgcggttgaa
    23761 aaacatcgcc actgccagcc cgagcacggt gctgcccgcc accaccagcg cggtatacca
    23821 gaccgtcatc cacagcgagt gccagaagcc gggatcggag aggatacgca catagttact
    23881 caccccaaca aaggttgact cgatgttggg attgagcggc agacgtaaaa aactgatttc
    23941 gatattcgac accattggcc aggccaccag accacccagc agtaataagc tgggcgcgag
    24001 aagcagcagc gcaaagggca tatcggaacg acctgaaaaa agcctgttca ttgcggattc
    24061 ctgtcctggg tttagtgctg ttcgatcagt tcatccagct ttttctggct cgcttgcagc
    24121 gtgccaggga gatcggcttt acccacggtg acgttatgca ccatggagct caccacgcca
    24181 gaccccgtca catcacccat gcgggtaaag tttttatccc ctactgcacc aaagacctga
    24241 atatttggca gttcaccgat tagctgattc ggtagttcac ccagcgcctt aataacgtcg
    24301 ttgtctttcc aggtggcggt agtgacaacc gctttattca ccggcagcgc agcacctggc
    24361 gacatcatca cccagtcggc aatgttgtct gcctgctcca taaaggtgac aaatttttct
    24421 gctgcttccg tctcttcggt cttttgcccg gcggtaatgg tcagcgaggt caacatgccg
    24481 tagaccgcag agtttttctc ggttggcacc acgaaaccga cgtttttcgg gtcgccttct
    24541 ttaatcacag ccggaaggat ataggtggag taaatcgcca tcggcgcggt gccgttcata
    24601 aaggcgtctt tcacttccat gatgtcgtta gaacccggca tagtgttggc agcaaggtcg
    24661 cggtaatagg tcagtgcctg catcatctct ggtgtatcaa gggtgatttt gccttcggcg
    24721 ttaaagacgt tagcctggtt ggataacgca aactgggaga aggattgttc cgtcaacacg
    24781 ctttctgctg taggcagcgc aatgccgtat tttttattcg ccgggtcatt cagtttctgt
    24841 gcaacgtcca gcagctgttg ccagtttttc ggctcctcaa gccccgcttt tgccagcaca
    24901 tctttgcgat accagatacc gccaatccag gcgctgacag gaacaccggt ccatgcgcta
    24961 ccatcttcgg tacgcacaat acgcagtacg ccatcgtaaa acgcgccttc accaacgttg
    25021 ctgatgactg tggcaaccgc tttgcgatcg ataagctgct ctttgtccat cactttggcg
    25081 tagtcatggc tggtttctat cacttccggc agcgaaccgc tacgtgaaag agtaatgact
    25141 ttagtgttat aggcatcttc ttccacgggc acttgcttaa cggtgatgcc agggttttct
    25201 ttttcaaaac gggcaatcaa tttactgata acggcctggc gctcctgctc caccgaagaa
    25261 tgcataaatt cgatggatac attcgtttta ttttcttctt tacaccctga aatcagggcg
    25321 caggaaacca gtgctgataa cagcacgatt tttgatttaa tcatgagatg ttccttttta
    25381 tttaatccac ataacctgcc aggggcgcag cgttatttca ttacccaata tatcaacttc
    25441 actaatcaga tcccgaccat gcagattagt aatatttaca tgctgaatat ttttactgac
    25501 attaaacaat ccagtcagac aattaccatc agcgttactt ctttgaatac gcattacgga
    25561 tgaattaatc gtatcgatgg taaaattatt atccggatga aactcgttat ggctgcgacg
    25621 aagtgtaatt aaacgcgaca actcatgata taccgcatgc cttaatgtag cttcatcgtt
    25681 cagttctcgg gttatctctt tactgtgata ttttttacgg ttaatcgcac ggttatatcc
    25741 gagtttttcg acacctgcgt aatcattacg cgagccaaga atactttgaa tatatatcgc
    25801 tggcacaccg gggaaactta acaaaatcgc atgggcgaga ataaacctgg cgcaacgttc
    25861 ttcatcgcta ctctcacggc ggcttaacgc atccatatag gtcacattta tttcatacgg
    25921 actgcgtgta ccgtcgggat tatttttcca gtttactaat gcaccttcct gctgtaacgc
    25981 ctcgaccagc tctaatattt cgctttcagg caacaagccc cgtagcgggt ttagcccgat
    26041 gccatcgtga gaggcgagga agttaaacca ggtggtgttg ctggaaggta gtgtcaggtt
    26101 ttgcgcccac gcacaaagcg cctcaacgtt ctgtttttgc accgcatgca gcaccagtgg
    26161 cggcagcgag aactggtaca ccatatgtgc ttcgtcatcg cctgcgccaa agtaagcaat
    26221 gttgtcttta tgcggaacat tggtctcggt aatgatcact gtacctggcg caacgttatc
    26281 aataatcgac cgtaacagtt tgataatcag atgtgttttt tccagatgga tgcagcttgt
    26341 tcccggctct ttccacataa agccaacggc atccaggcgg acatactcag cacctttcgc
    26401 aaggtaacag agtaaaacat ccaccatcgc cagcaacact tcagggctac ggtaattcag
    26461 gtcaatttga tcgtcactaa aggtggtcca taaatggcgc gttgaatgat cgcgcatctg
    26521 gaatggcgtt aataacggta acgcacgcgg gcgagtgacg gcgctgagat cggtttgcgg
    26581 gtcaacggca ataaaaaaat cttcaaaacc tggatgctgt tgtaaatagt ttttaaatca
    26641 ttcacttttt gccgacatat ggttgcagac aaaatcaaac attaaatgac tgcattcacc
    26701 gagttgctga atatcctgcc actcccccgc ttcactggcg acctgatgat aatcaattac
    26761 cgaaaagcca tcatcagatg accatggata aaacggcaac aaatgaacat gtgaaaaaat
    26821 gctttgcagc cattgatggt aaaactgatt aaatgtgggt aatggtttta aatcattgct
    26881 gtgaaattga tcggcatagg taattaacac gacatcactt tcatcccagt gttttttacg
    26941 tcgctgtgta attaatcgtt tcgcactctc aagacgcgtt acaagtttct gtaaatgagt
    27001 tgcggtaaat gttccaccgt agatttcgtc caggtaatcc gtaattttct gtttcatgtt
    27061 gcctttccgt ggtagcggtc ccatgaaacg agagactact ccgcagaaaa taaactgtca
    27121 attgagcaca agggttttag tcacgcagga aatcattttt taccagggaa aaagcgtatt
    27181 tgtgagctga cgcaaataat ctatggggat gtaaataagg tgtgacagaa aaaaaacggc
    27241 gcataagcgc cgctcatggt gaattctttt aacctttgac cttcggcatt gcgatgtctt
    27301 tcaggccacc ggcgttctca acgtgggtat atcccatctc gctaaggatc tcttttgctt
    27361 gccctgactg gcgtccggca ttgcaataca ctttcacggt gtcgttttta tccggaacgg
    27421 cggtggcaat gcgctctttc acttccttca ggggaatatt gatggccccc tgaacgtgct
    27481 cttgctgata ctgctctgga acacgaacat cgatccagtg ttcagcggcg aaaacgggca
    27541 gtgaaaacac cagtgccaga gcaagtaagc cttttttaaa catgtaagcg tccttctatg
    27601 gatgaaaatt gccatccatt atacgcctga gatttaacaa tttttgcgaa actcccctca
    27661 ccttttataa cgctgtgcca gtttattagc agcccgactt aacagcggtt ccagcgccac
    27721 agccagcaac attttcagcg gtcggcgagc aactgatttt atcgcccagc ccgctacacc
    27781 cgccgggcca tagcgcagtg cggtaagaag taccagcttg cctgctaatt tgaaaccagg
    27841 ctttaccttt tgcccggcct gttgccagcg agtattcata actttcctca cagttgacgg
    27901 aaacggctac gtaacgtgaa agtatcggaa gtgacataac gttccatctc gcgtaaacgc
    27961 gtttcacttg ccgccagttc acggtcgact tcatccagca attcgctgct ggaaggtagc
    28021 tgctcaccaa aggccatgtt gtccggcatt ggatcaagcg caaatgacaa aatgatgtaa
    28081 gcaaccaggg taaacagcgc cagaccgaag aaaatcgaca gcaccaccag gatacgcacc
    28141 agttttgccg gtacatcaaa atagttggca atcccggcgc agacgccgcg gaccatgccc
    28201 tgctgtggaa tacgccataa ttttttattg agattaatgc ccgccattag cgatccctcc
    28261 agttcggatg ttctgcatca agaatagatt ccagcgcctg aatacgttcg cgcatccgtt
    28321 ttgcttcatc agccagttgc gctaatcgct gctgctcact ttgcgacaat tcactgcgac
    28381 cagaacgatt gctgtaatgc agccataacc agatcggtaa aacaaacagc acaaaaatgg
    28441 ttaacggaat agccagaaat agcgcgctca taagtactcc ttagacgatg agcggcggcg
    28501 cgttcggacg ccgcctggat gttattattg attgtcttgc ttcattttgg cttttaattg
    28561 tgccagttgt tcgctgattg catcatcggc tttcagttcg gcaaactgat cgtccagcga
    28621 tttttgttta ccgaagctgt ggctttctgc ttccgcttcc atctggtcaa tacgacgttc
    28681 gaaagattcg aaacgagcca ttgcttcatc cagtttgcca ctgtccagct gacgacgcac
    28741 atcgcgcgac gagtttgccg cctgatgacg taacatcaat gcctgctggc gagcgcgtgt
    28801 ttcgctcaat ttgttttcca gctcaccgat ctctttcttc atgcgtgcca gcgtgtcgtc
    28861 caccagcgtc acttcatgtt ccaggctctt aatcagatcg gtcagtttct gtttttcaat
    28921 taacgctgca cgtgccagat cctctctctc tttcagcagc gccagttcgg ctttttcctg
    28981 ccattcaact tcacgcgccg acgcttgttc aatacggcga gtcagctgtt tcttttctgc
    29041 caacgcgcgc gccgaagtag aacgtacttc aaccagtgta tcttccatct cctggatcat
    29101 cagacgaacc agtttctgtg gatcttccgc tttctctaac agagcgttga tgttggcatt
    29161 cacgatgtcg gcaaagcgag aaaaaatacc cataatgttg tcctcttgat ttctgcgttc
    29221 aggattgtcc tgctgaactg ttaatacaat ttgcgtgcca attttttatc tttttgattt
    29281 ataaagatct gattgaagaa ccaacagcaa catgccagga tgagttagcg aattacacta
    29341 acaagtggcg aatttcatca tggcagaata caaagataat ttacttggtg aggcgaacag
    29401 ctttctcgaa gtgctggaac aggtttcgca tctcgcaccg ctggacaaac cggtgctcat
    29461 catcggcgaa cgcggcaccg gtaaagagct gattgccagc cgcctgcatt atctctcctc
    29521 ccgttggcaa gggccgttta tttcccttaa ctgcgcggcg ttaaatgaaa atctgctgga
    29581 ttccgaactg tttggtcacg aagcgggggc gtttaccggt gcgcaaaaac gtcatcctgg
    29641 gagatttgaa cgtgccgacg gcggtacgct atttcttgat gaactcgcta cggcaccgat
    29701 gatggtgcag gagaaattat tgcgcgtgat tgagtacggt gaactggagc gcgttggcgg
    29761 cagccagcca ttgcaggtga atgtgcggtt ggtatgcgcg acgaatgccg atctcccggc
    29821 gatggtcaat gaaggcactt ttcgcgctga cctgctcgac cgactggctt ttgatgttgt
    29881 acaactgcca ccactgcgcg agcgcgaaag cgacataatg ttgatggcag aacactttgc
    29941 catccagatg tgtcgggaaa ttaagctgcc tctgttcccg gggtttacag agcgcgccag
    30001 agaaacattg ctgaattatc gttggccggg aaatattcgt gaattgaaaa acgtggtgga
    30061 acgttcagtg tatcgccacg gcaccagcga ttatccgctt gatgacatca ttattgatcc
    30121 ctttaaacgg cgtccgcctg aagacgctat cgccgtttca gaaaccacct cgcttccaac
    30181 actgccgctg gatttacgtg agtttcagat gcagcaggaa aaagagttgc tgcaactcag
    30241 tttgcaacaa gggaaatata accagaagcg tgcggctgaa ttactggggt taacttatca
    30301 tcagttccgc gcgttgttga aaaagcacca gatttagcga agttgagcaa tacaacttgc
    30361 cggatgcggc gtgaatgctt tattcggcat acataatcaa tacgtcgttt gcaggcctga
    30421 taagcgcagc gcatcagaca ttattgcgtt gggctattaa tcgctcagcg catcctgcaa
    30481 aattttcatt gccgcatcga attgcgcatc cggtatggtc agcggataca ggaagcgaat
    30541 cacgttgccg tatgcgccac aggtcagcag gagcagcccc tgcgccagcg cgcgttgctg
    30601 gattttctgt gcaatcgccg ctgacggctc gcccgtttgc ggatcgttaa actctgccgc
    30661 aatcatcgac cccaggccgc gtaccgcagc aatggccgga acgctttctt tggcatcaat
    30721 caacgtgttt ttgagacgct ggcccagttg attcgcgcgt tcgcagagtg attctttgtc
    30781 gataatattg agcaccgcgt gcgcggcagc caccgccagc gggttcccgg cgtaggtgcc
    30841 acccaacccg cccggcgcgg gcgcgtccat aatattcgcg ttaccgacca cgcccgaaag
    30901 cggcatcccg cccgcgaggc ttttcgccat cgtcattaag tccggcttat cggcgtaatg
    30961 atccatggca aacagtttac cggtacgcgc aaagccgctt tgcacttcat cggcaatcat
    31021 cacaataccg tgctcgtcgc acaggcggcg aatggcggca accagctctt ttggcgcaac
    31081 gttgaaaccg ccctcgccct gcaccggttc gaaaataatg gccgcgacct gcttcgcttc
    31141 gatgtctgat ttaaacaagc gttcgatggc gtcgagggag tcctgtgttg aaatgccgtg
    31201 taaatctgac ggataaggta cgtgatacac cgaaccaggg aacgggccga agccgatttt
    31261 gtacggcgca acttttccgg tcagcgccat ggtcatatat gtacgaccgt ggaagccacc
    31321 gctaaacgca atcacgccag gtcgtccggt atgggcgcga gcaattttca ccgcgttttc
    31381 caccgcttcc gcaccggtag tgaagaatgc ggttttagcc tgcccgctca ctggggcaag
    31441 ggcgttaatt ttctccgcca gcgtgacgta gctttcgtac ggcacaatct gatacgccgt
    31501 gtgggtaaac tgttgcaatt gctgctccac cgccgcgacc agatcagggt ggcgatgccc
    31561 ggtattcagc accgcaatgc ctgcggcgaa atcgatgtat tcgttgccct ctacgtcctt
    31621 cagcgtagcg ttttctgccg actgggcgaa gaagttacac atcacgccaa ccccgcgtgg
    31681 ggtagcagaa agacgacgct gatggaattc attgttgctc atgtttcaca ccttttgact
    31741 cttcactgtt aatcggaaat taaacgccca gacgatcgcg caggctgtaa taagccgcgc
    31801 ccatcgcggt aaatggcacg cgcagcgtgc gcccgccggg gaacgggtaa tgcggcagat
    31861 tggcgaaggc atcgaaacgt tcggcgtcgc cgcgcagcag ttcggcaatc agacgcccgg
    31921 caagatgggt acaggtgacg ccgtggccgc tgtagccctg catgtaatag atgttggtat
    31981 caaggcgacc gaactgcggc atgcgcgaca aggtcagcag gaagttgccc gtccagcggt
    32041 aatcaatttt cacgcccttc agctgcggga aggttttcag cagtttcggc accacaaggc
    32101 gctcaacgtc atccgggtcg cgcgcgccgt agaccacgcc gccgccgtac agcaggcggt
    32161 tgtcggcggt aagacggtag taatcaagca gatagttaca gtcttccaca cagtagtttt
    32221 tcgggatcag cgaacgggct aaatcttccg acagccgttc ggtggtgatc acctgcgtgc
    32281 cgcacggcat gctgcgtttc gccagttccg gctctacttt atcgcccaga tacgcattcc
    32341 cggcgacaat cacatacttc gccgtcacct gacctttggc agttcgcaca acggctggcg
    32401 tggtgtgctg gatctgcgtc acggcagaaa gttcatacac gcgcccgccg ttgaggcgga
    32461 tggcgtccgc ttcgccaatg gcgaggttta gcggatgaat atgcccaccg ctgtgatcca
    32521 gcagcgcacc ggtatagcga tcgctggcta cttcacggcg aatggcgttg gcgtccagca
    32581 attccagctg tttattgccg tagcgttccc agttctcttt ctgctcttca agtgtggcga
    32641 gctgtttatc attcatcgcc acaaacaggc cgccggggcg gtagtcgcag tcaatctgat
    32701 aacgtttgat acgttcgcgg atgatctcac caccttcgaa catcatgctg ccgagcatac
    32761 gggcggtgtc catgccgtag cttttttcga tcacgtcgat gtcgcggctg taggagttca
    32821 caagctgccc gccattgcgc ccgcttgcgc caaagccgat gcgtgaggct tcgagaacca
    32881 ctacgtcaaa gcccgcttcc gccagatgca gcgcggaaga gagcccggta tagccgccgc
    32941 cgaccacgca aacgtcgcag gaaatcgact cattcagcgt gtcgaatggc gcgtatttat
    33001 tcgcactggc ggcgtagtaa ctgctggtat gttcggtcat ttcaggcctc caggcttatc
    33061 cagatggttt tcagttcggt gaatttttca agggcatgca gggatttgtc gcgaccgttg
    33121 ccgctctgct tatagccgcc aaacggcacg gtcatatcgc cgtcgttgta gttattgacg
    33181 aagacggaac cagccttcag gcggcggctc atgcggtgcg cgcgggagag gtcgcgcgtc
    33241 cataccgccg cgccaaggcc gtactggctg tcgttggcaa gctgtagcgc ctggtcttct
    33301 gatgtgaaac gcgtgaccac cagcaccgga ccgaaaatct cttcgcggct taaggacgca
    33361 ttcgggtcta catccacaaa gatggtcggg ccgatggcgg cagccagctc agcgttacga
    33421 ccatccagca gcagttgccc tttgctttcg ccttcccgaa taaagctatg gaccgagtca
    33481 gcgtgagcgc tgtcgattaa ggtgcccatg gtggttgcgg gatcaagtgg atggcccggc
    33541 tgccagtttt gcgcctgctg ttttaacagg gctaagaatt catcggcgat gctctcttcc
    33601 agtaacaaac gcgttccggc gatgcacacc tggccctggt tgtagaagat gcctgcggcg
    33661 gtggcgcttg ccgctttttg cagatccgga caatcggcga aaacgatgtt ggcgcttttg
    33721 ccgcccgctt ccagccagac gcgtttcatg ttgctctcgc ccgcatcttt cagcagctgt
    33781 ttcccggtac gggtcgaacc ggtaaaggca atggcgtcga tatcgttatg acgcgacagc
    33841 gcctgccccg cttcatgacc aaaacctgtc accacgttca aaacaccatc cggcaagcct
    33901 gcttctttcg ccagccccgc gagacgaatc gcactgagcg gtgatttttc agacggtttc
    33961 aggacaacgc tgttccctgc tgccagcgcc gggccaagct tccagcaggt cagcagcagc
    34021 ggaaagttcc acggtacgat ggcggcaatc acaccgaccg gttcacgcac gatcatcgcc
    34081 agctcatggc tactggtggt cgccacttcg ccatacactt tgtcgatcgc ttcggcgtac
    34141 cagcgaatgg cgcgcgccgc gccgggaata tcatcacgca gactgtgacg aatcggtttg
    34201 ccggtgtcga gagtttccag cagtgccagc tcttcggcgt gggcttccat taaatcggtg
    34261 agtttattca gtaccgcttt acgtttagcc ggagaagaga gtgaccagtc gccgcgttca
    34321 aatacgccgc gtgctgcgct caccgcacgg tcgatatcga cgctcttgcc gcgggcaatt
    34381 ttcgccagcg gtgcctgggt gaccggatca acggtttcaa aggtttcatt ttccgccgca
    34441 gcagtatatt caccgttaat aaataagcgg gtttcaatgg cgagacttaa cgctttatcc
    34501 tgccagtaag ccagatgatg aaaattcatt atgactcctg tttcacgtct atcagatata
    34561 tgcagtttgt gaacgtcagg gcgcagagat ccccgggccg tatgcccgtt tatttctggc
    34621 gacattcttt atatacactt catccttcaa gccgcctctg ccttggctgc gttcgctccc
    34681 ccgaatcact tacctgagta aactcatcgg gattcgctca cttgccgcct tgatgcaact
    34741 tgaatgattt tgtgtataga ttgccgggcg cattgcgctt acccggctta cagaacaaaa
    34801 agattaaaac gtggtgggcg tatgggcgct gataattcgg caaatacctg ccgacgtatt
    34861 actgaaactg tgcgggatgc cggtattaat ggcataactt tgccccgcga cgaggtggta
    34921 atcctgacca ttaatcgtca gaacaatttc accttccagt actgtgccta tttcctcacc
    34981 ctgatgctta attctttccc cagtggttgt gcccggctgg tacgtttcaa agatcatcgc
    35041 cagcgtgcga ttcgggttac cgttatgaac cagcttcatt gacacaccct gactgcccat
    35101 ctcaattaag tcgtcctgat taatgacgac ctgcggctca tcaggttttt ccggctcgga
    35161 aaagaattcc gagagtgaca gaccatacac cttcagcagc ttttgcagcg tactgatggc
    35221 agggctgact ttatcttgtt ctatcgtact gatagcactg tgagtcagcc cggagagttc
    35281 ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt tccgacaagc gttttcctgg
    35341 cgccagtccc tcatcactca tatttgcatt tccttaaact gtagtggtca gagtcgctgt
    35401 ttttcagcga tatggtgctg acaagcggtg ataaagccct cgaacaatat acgcgaaagc
    35461 gcgtactcgc tactgttcca ttccgggtgc cactgtacgc ccagcgcaaa aggatgattg
    35521 atgacgctaa ccgcctcaac taagccatcc ggcgagcgag cttcaacacg caaccgtggg
    35581 ctaacgacct tcgccccttg cccatgcaga gagtttaccc aaaagttgct acattcaggt
    35641 aacaacgcag acagtaatcc cccctcttca acctgaactt catgcgacgg tgcatattgc
    35701 tgttccaccg gcagttcggg atcttcccga tgttccagca attcaggctg ttcgcacagc
    35761 ttgcgatgca acgaaccacc ggttgccacc accagttcct gtaaaccccg gcagatggcg
    35821 aaaatgggga tgcgcctttc gagtgcggca ttaattaacg ccatgctcag aagatcacgc
    35881 ccgggatcgg cgtcaggctc atcgccgttt tcaccatata ggtgcggctg cacattgctg
    35941 ggactaccag gaagataaat gccatcgagt ttcggcaaaa gttgttcaag taatgacggt
    36001 tccgccagcg catgtggtag cgcaattggc aagccgcctg cgtggatgat ggcattcagg
    36061 tacttttctt gcagagtctg ggtcgcatga cccttaagcc tgttcctgca cattacgaca
    36121 ccgataaccg gattgttcat tatattttcc atgctcaatc tcacaaagtg gactaaatta
    36181 tcgccattac tgcggtttaa atgaccttta tgttcaatat tttttcaatc tagcagtggg
    36241 ttttcatttt tgcaaactca atttaacatt tgacaaacat ttagtttgca tacagattcg
    36301 aatggtggtc attatatttt acgctttgat aacgagcgga aaacaaacca aaggcgaaga
    36361 atcatggaaa ccaatatcgt tgaagtagag aactttgttc agcagtcaga agagaggcgg
    36421 ggtagcgcct ttacgcagga agtgaaacgc tacctggagc gctacccgaa tacgcaatat
    36481 gttgatgttt tacttactga tttgaatggt tgcttccgtg gaaaacgtat ccctgtttca
    36541 agcctgaaga agttggagaa agggtgttat ttcccggcct cggtgtttgc aatggatatt
    36601 ctgggcaacg tagtagaaga agcgggtctg ggtcaggaaa tgggcgagcc ggatcgtact
    36661 tgtgttcctg ttctcggttc cttaacccct tctgccgccg atccagagtt tatcggtcag
    36721 atgctcctga ccatggtcga tgaagatggc gctccctttg acgttgagcc gcggaacgtt
    36781 cttaaccgcc tgtggcagca gctacgccag cgcggattgt tcccggttgt agcggtagag
    36841 ctggagttct atttactgga tcgtcagcgc gacgctgaag ggtatctgca accgccctgc
    36901 gcgcccggca ccgatgaccg caatacacaa agccaggttt actccgttga taacctcaac
    36961 cacttcgccg atgtgctcaa tgatattgat gaactggcgc agttacagtt aattcccgcc
    37021 gacggtgcgg tcgccgaagc ctcgccgggt cagtttgaaa tcaaccttta ccataccgat
    37081 aacgtgctgg aagcctgcga tgatgcgctg gcgctaaaac gcctggtgcg tctgatggca
    37141 gaaaagcata agatgcacgc cacttttatg gcgaagccgt atgaagagca cgcgggcagc
    37201 ggaatgcata tccatatcag tatgcaaaat aaccgtggcg agaacgtgct ttctgacgcg
    37261 gaaggcgaag attcgccgct gctgaaaaag atgctcgccg ggatgattga cctgatgccg
    37321 tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc gccgcttcca gccgggaatg
    37381 tatgtgccga cgcaggcgtc gtggggccat aacaaccgca ccgtcgccct gcgtattccg
    37441 tgcggcgacc gtcataatca ccgcgtggaa tatcgcgtgg cgggtgccga tgccaaccca
    37501 tatctggtga tggcagcgat ttttgccggt attttgcatg gccttgataa cgagctgccg
    37561 ttgcaggaag aagtcgaagg caacgggctg gaacaggaag gcttaccctt cccgattcgc
    37621 cagagcgatg ccctgggtga gtttatcgag aatgatcacc tgcgccgcta tttaggcgaa
    37681 cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc tgttgcagtt tgagcgcctc
    37741 atcacagaaa cagaaattga gtggatgttg aaaaacgcgt aattccccat gcggggttgt
    37801 ttcatcggcc tgagcggagc caggcctgac atttgctggt aatccagaca tttcccggat
    37861 gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa cgattaagac tgtgacgagg
    37921 aaatgagatg atgccgatga ttaaatcccc gcatggcgag ggaggctgcg tatgcgcccc
    37981 tcctgctact gactggactc cgcccccttt gcttcccttg ctaaacaggt ttgatttcag
    38041 gtcgacacga ccgcaaacct tattacgcag gggaggcagc aattatggct attaattcac
    38101 cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg aaaatcactg aaattgtggc
    38161 aggtggtaat gatgggtctg gcctatctca cgccgatgac cgtatttgat acttttggta
    38221 ttgtctccgg cattagcgac ggtcacgttc cagcgtccta tttgctggcg ctggcgggcg
    38281 tgctgtttac cgctatcagc tacggcaaac tggttcgcca gtttccggag gccggttcgg
    38341 cctataccta cgcgcaaaag tcgattaatc cgcacgtcgg atttatggtc ggctggtcat
    38401 cactgctgga ttatctcttt ttgccaatga tcaacgtttt gttggcgaaa atctatctct
    38461 ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt aaccttcgtc gccattttaa
    38521 ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt caataccctg tttgtactgg
    38581 tgcaaatctc catcatggtg gtgtttatct tcctggtggt tcagggactg cataaaggag
    38641 aaggcgttgg caccgtctgg tcacttcagc cgtttatcag cgagaacgcg cacctgatcc
    38701 cgattattac cggtgcgacg attgtctgtt tctcgttcct cggtttcgat gcggtgacca
    38761 cgctttcgga agagacgccg gacgccgcac gcgtgatccc gaaagccatc ttcctgacgg
    38821 cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt tatgcagctg ttctttcccg
    38881 atatcagccg ctttaaagac ccggacgccg cactgcctga aattgcgctg ttcgtcggcg
    38941 gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt gaacacatta gcgtctggcc
    39001 tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat ggggcgtgac aatgtgtttc
    39061 cggagcgcgt gtttggctat gtgcacccaa aatggcggac tccggcactc aacgtcatta
    39121 tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt agtcaccgcg acagcactga
    39181 ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct gtcggtgttt aatcatttct
    39241 ggcggcgtaa aggaatgaat aaaagctgga aggatcactt ccactatttg ctgatgccgc
    39301 tggttggcgc gctgacggtg ggtgtgctgt gggttaacct agagtcaacg tcactgacac
    39361 tcggtctggt atgggcttcg ttaggcggcg catatttgtg gtatctgatc cgccgctatc
    39421 gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag cgaaacgtaa tagccggatg
    39481 cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca ttaactataa aaagatgtat
    39541 gacccggatc acgagtgcta tcatccgccc ttatgcttag cgcattaacc ccaggggcaa
    39601 ataatgaacc acgatatccc gctaaaatat tttgatattg ccgatgagta cgcgaccgaa
    39661 tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg cccactactt ccagttactg
    39721 ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag cccaacatga aatggctgct
    39781 gaagcgggga ttaatcccgt acgcattgat gagatcgcag agtttttgaa tcaatggggt
    39841 aatgagtaat tatttaattg cagacgatcg gtcagtatcg ttgtccggat aaagcgttca
    39901 cgccgcatcc ggctaacaat gacacgcact gcagcgtgct ttaatgggtt tctcacgatc
    39961 gtaccgctct gctcctcgct aagttgttct gtgagttata gttatagaga aatcacgggt
    40021 acgaatgttg aacagatggc gacgccacct gttcaacgac caaccacacg gccctcatta
    40081 cgacccctga acacaaacgg taatggcaaa tgtcattaaa tattagtaag atagcctctt
    40141 tacataaaat cccttaatat tatgcgccag gtattatcgt ctcttttggt gattgctgga
    40201 cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc cgcatgctga tatccgcgac
    40261 agcggttttg tctattgcgt cagcgggcaa gtcaacacct ttaacccatc caaagcgagc
    40321 agtgggttaa ttgtcgatac ccttgccgcc cagttttatg atcgactgct ggatgtcgat
    40381 ccctatacct atcgcctgat gccggaactt gccgaaagct gggaagtact cgacaacggc
    40441 gcgacctatc gcttccacct gcgtcgcgat gttccgtttc aaaaaaccga ctggtttact
    40501 cccactcgta aaatgaatgc cgacgatgtg gtgtttacct tccagcgaat ttttgaccgc
    40561 aacaacccgt ggcataacgt caacggcagc aacttcccct acttcgacag cctgcaattt
    40621 gccgataacg tgaaaagcgt ccgcaaactg gataatcata ccgttgagtt ccgactcgcc
    40681 cagccggatg cttctttttt gtggcacctc gcaacccatt atgcttcggt catgtcggca
    40741 gaatatgccc ggaagttaga gaaagaagat cgccaggagc aactcgaccg tcaaccggtc
    40801 ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc aatttattcg cctacaacgt
    40861 catgatgact tctggcgcgg taaaccgtta atgccgcagg tagtggtgga tttaggctcc
    40921 ggcggcaccg gacgtctgtc gaaactcctg accggggaat gcgacgttct ggcctggcct
    40981 gctgccagcc agctatccat tttgcgtgac gacccgcgct tgcgtttaac gctgcgtcct
    41041 gggatgaacg tcgcctatct ggcatttaac accgccaaac cgccgctaaa taatcccgct
    41101 gtccgccatg cactggcact ggcgattaat aaccagcgcc tgatgcaatc catctattat
    41161 ggtacggctg aaacggcggc ctctatttta ccgcgcgcct cgtgggccta tgacaacgag
    41221 gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac agttgaaggc gttggggctg
    41281 gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc aggcgtggaa ccccagtcca
    41341 ctgaaaactg ccgaactgat tcaggcggat atggcgcagg ttggcgtaaa agtggtgatt
    41401 gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg atatgagcca tgatctgacg
    41461 ttatccggtt gggcgacgga cagtaacgac ccggacagtt tcttccgtcc tttactgagc
    41521 tgcgcggcaa ttcattcaca gaccaacctc gcccactggt gcgatccgaa attcgacagc
    41581 gtgttgcgta aggcgctctc ctcgcagcag ctggcggcgc gtattgaagc ctatgacgaa
    41641 gcgcagagta ttctggcgca ggaattgccc attttgccgc tggcgtcgtc attgcgtttg
    41701 caggcctatc ggtacgatat caaaggtctg gtacttagcc cgtttggtaa cgcctccttt
    41761 gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac catgattatc ttcaccttac
    41821 gccgcatttt gttattgatt gtcaccttgt tcctgctgac cttcgttggc ttcagcttga
    41881 gctatttcac ccctcatgcg ccgttacaag gcgcgtcact gtggaatgcc tgggtgttct
    41941 ggtttaacgg cctgatccac tgggattttg gtgtttccag cattaacggt caaccgattg
    42001 ccgaacagtt aaaagaggtc ttcccggcga cgatggaact gtgcatcctc gccttcggtt
    42061 ttgcgctgat tgttggcatc cccgtgggga tgattgccgg gattacgcgc cataagtggc
    42121 aggataactt gatcaacgcc atcgccttac tgggcttttc aatcccggtg ttctggctgg
    42181 cgcttctgtt gacgctgttt tgttcactca cgctaggttg gttgcccgtt tcagggcgtt
    42241 tcgatctgct ctacgaagtg aaaccgatta ccggttttgc gttgattgat gcctggcttt
    42301 cggactcacc atggcgggat gaaatgatca tgagcgcaat ccgccatatg atattgcccg
    42361 tgatcaccct gtcggttgcg ccaacaactg aagtgatccg cctgatgcgt atcagcacta
    42421 tcgaagtgta tgaccagaac tatgtaaaag cggcggcgac ccgcggtttg tcgcgcttta
    42481 ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc ggttattcct cgcctgggct
    42541 tacagttttc caccatgctg acgctggcga tgatcaccga aatggttttt agctggccgg
    42601 gtttagggcg ctggttaatt aacgccatcc gccagcagga ttacgcagcc atttccgccg
    42661 gagtgatggt gtgtggctca ctggtcatta ttgttaacgt gatttctgat attttgggtg
    42721 ccatggctaa ccctctgaaa cataaggaat ggtatgcctt acgatagcgt atacagcgaa
    42781 aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat tttatagtga cgcctctgcg
    42841 atggtcggcc tgtacggctg cgcggggctg gctgtactgt gtatttttgg cggctggttt
    42901 gcgccttacg gcatcgacca gcaatttctc ggttatcaat tattgccgcc gtcatggtcg
    42961 cgctatggcg aagtttcttt cttcctgggg actgacgacc tcggacgcga tgttttaagc
    43021 cgtttactga gcggagctgc gccaaccgtg ggtggcgcat ttgtggtgac gcttgccgcg
    43081 acgatctgtg gcctggtact gggcaccttc gccggggcga cacacggcct ccgctcagcg
    43141 gtgcttaacc atattctgga taccttactg gcgatccctt cgctgctgtt ggcaattatc
    43201 gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt ttgctgtctg gctggcattg
    43261 ctgccgcgta tggtacgttc gatttacagc atggtgcatg acgaactgga aaaagagtac
    43321 gttatcgccg cccgtctgga tggcgcatca acgctgaata ttctctggtt tgctgtgatg
    43381 ccaaacatca ccgccgggct ggtcaccgag atcacccgcg cactgtcgat ggcaattctc
    43441 gatatcgccg cgctgggctt tctcgatctc ggcgcacaac tcccctcgcc tgaatgggga
    43501 gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc cgtggactgt catgctgcca
    43561 ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc ttggcgatgg cgttcgtcgt
    43621 gcgattattg cgggggtgga ataatgccat tactcgatat tcgtaacctg accattgaat
    43681 ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt aagtatgacc ttaaccgaag
    43741 gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa gagtttgatt gcgaaagcga
    43801 tttgtggggt gaataaagat aactggcgtg ttactgctga ccgtatgcgt tttgatgata
    43861 tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact ggttggccat aacgtgtcga
    43921 tgattttcca ggaaccgcag tcgtgtcttg acccttcaga acgtgtgggt cgccagttga
    43981 tgcaaaacat cccagcctgg acctataaag gccgttggtg gcagcgtttt ggctggcgca
    44041 aacgccgtgc cattgaactg ctgcaccgcg tggggattaa agatcacaaa gatgcgatgc
    44101 gcagttttcc ctatgagttg accgaaggtg aatgtcagaa agtgatgata gccattgcac
    44161 tggcgaatca accgcgtctg ctgattgctg acgaaccgac caactcaatg gagccaacaa
    44221 cccaggcgca aatctttcgc ctgctgacgc gtctcaacca aaacagcaat accactattt
    44281 tgcttatcag ccatgactta caaatgctta gccaatgggc ggataaaatt aacgtgcttt
    44341 actgcggtca aacagtggaa accgcgccga gtaaggagtt ggtgacgatg ccacatcatc
    44401 cttataccca ggcgctgatc cgcgcgatac cagacttcgg cagcgcgatg ccgcataaaa
    44461 gtcgcctcaa tacgctgccc ggcgctatcc cactgctgga acagttaccg attggttgtc
    44521 gtctggggcc acgttgcccg tatgcacaac gagaatgcat tgtgacgcca cgtttgacgg
    44581 gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa catggagaaa gagtgagatg
    44641 atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt tccgctaccg gaccggctgg
    44701 tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct ttacgctacg tgaaggccag
    44761 acactggcga ttattggcga gaatggttcg ggtaaatcca cgctggcaaa aatgctggcg
    44821 ggaatgatag agcctaccag cggcgagtta ttgattgacg atcatccact gcattttggc
    44881 gattattcct tccgcagtca gcgtattcgc atgatttttc aggatccttc gacctcgttg
    44941 aatccccgtc aacgtatctc gcaaattctg gattttccac tgcgcctgaa caccgacctg
    45001 gaaccggaac agcgtcgcaa acagattatt gaaacgatgc gtatggttgg gctgctaccg
    45061 gatcacgtca gttactatcc gcatatgttg gcacccggac aaaaacagcg tctgggtctg
    45121 gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg atgaagccct cgcttcactg
    45181 gatatgtcga tgcgttcgca gttgattaat ctgatgctgg agttacagga aaaacagggc
    45241 atttcgtata tttatgttac ccagcatatc ggaatgatga agcacattag cgaccaggtg
    45301 ctggtgatgc atcagggcga ggttgtcgaa cgaggcagca ccgcggatgt gctggcatcg
    45361 ccgctgcatg aactcaccaa acggctgatt gccgggcact ttggcgaagc attgacagcg
    45421 gatgcgtggc gtaaagatcg ctaattttct gaatcctcct tccagattat ccacctcatt
    45481 ctgacaagag attaaatctt gtcagaatga ggtgatggat aaaataaaat caaatgcgcg
    45541 tgatttaaga cgcaatctca ctttgcagga acgaaagctc tggcgatatc ttcgcagccg
    45601 acgttttggt gatttcaaat ttcgccgtca acatccggtg gggagctaca ttctcgattt
    45661 tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt gggcagcatg atttggcagt
    45721 tgcctatgat accaggcgca ctagctggct tgagtcgcag agctggaccg tgctgcgttt
    45781 ctggcataac gagattgatt gtaatgagga ggcggtgctg gagattattc tgcaggaact
    45841 gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc gaccgagccg aatagctgtt
    45901 gtggtgaaaa catggagaca gtgctggaga atattcggca aggtctgaac catcccagcc
    45961 atcgccataa aagggttagg ggctgtatga gcctgtttgt tgctggggta acaatatttg
    46021 cacaatatgg tcccctcgcc cctctgggga gagggttagg gtaaggggaa aagcgcccac
    46081 cctgccgcag cctgctccgg tcggacctgg caactatagc tactcacagc caggttgatt
    46141 ataataaccg tttatctgtt cgtactgttt actaaaacga cgaatcgcct gattttcagg
    46201 cacaacaagc atcaacaata aggattaaag ctatgggttt tctttccggt aagcgcattc
    46261 tggtaaccgg tgttgccagc aaactatcca tcgcctacgg tatcgctcag gcgatgcacc
    46321 gcgaaggagc tgaactggca ttcacctacc agaacgacaa actgaaaggc cgcgtagaag
    46381 aatttgccgc tcaattgggt tctgacatcg ttctgcagtg cgatgttgca gaagatgcca
    46441 gcatcgacac catgttcgct gaactgggga aagtttggcc gaaatttgac ggtttcgtac
    46501 actctattgg ttttgcacct ggcgatcagc tggatggtga ctatgttaac gccgttaccc
    46561 gtgaaggctt caaaattgcc cacgacatca gctcctacag cttcgttgca atggcaaaag
    46621 cttgccgctc catgctgaat ccgggttctg ccctgctgac cctttcctac cttggcgctg
    46681 agcgcgctat cccgaactac aacgttatgg gtctggcaaa agcgtctctg gaagcgaacg
    46741 tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg tgttaacgcc atctctgctg
    46801 gtccgatccg tactctggcg gcctccggta tcaaagactt ccgcaaaatg ctggctcatt
    46861 gcgaagccgt taccccgatt cgccgtaccg ttactattga agatgtgggt aactccgcgg
    46921 cattcctgtg ctccgatctc tctgccggta tctccggtga agtggtccac gttgacggcg
    46981 gtttcagcat cgctgcaatg aacgaactcg aattgaaata atcattctgt tggtaaatat
    47041 tgggcggcgt tctgccgccc gttatctctg ttataccttt ctgatatttg ttatcgccga
    47101 tccgtctttc tccccttccc gccttgcgtc aggataacga tttcctttac gaccaaggag
    47161 cgcccatgga acaacgccac atcaccggca aaagccactg gtatcatgaa acgcaatcca
    47221 gtactgcgga gtatgacgtt ctgcctctgg tcccggaagc cgcaaaggtc agcgatccct
    47281 ttctgctcga cgtgatcctt gatgaagaaa cgctggctcc cttcctttca tggctggtcc
    47341 ctgcgcgcgt tcttgcagtg gaattgttcc ctgaccagct taccgtgacc cgttcacaga
    47401 ccttcaccgc ttatgaacgc ttgtctacgg ccctgacggt tgctcaggtt tgcggcgtcc
    47461 agcggttatg taactactat tcggcgcgac ttacgccgct ccccgggcct gattcctcca
    47521 gggaaagcaa tcataggttg gcacaaatca cgcaatatgc ccgccaactg gctagctcgc
    47581 cttctattat cgacaaccga tcgcgccagc atctgaatga cgtcggtctt actgccgggg
    47641 actgtgtgat cattaaccaa atcattggtt ttattggctt tcaggcgcgg accattgcga
    47701 catttcaggc ttatctcggg cacccggtac gctggttacc cggtctggaa atacaaaact
    47761 acgccgacgc gtcactgttt gctgatgaat caatacgctg gcgaagcagc tatgaggtgg
    47821 aaaaactacc tgaagagtac acaaaaagtt caacagcaga actttgccaa ctggccgaaa
    47881 cactctctct ccaccctatt tcactttccc ttctcgaaag gttgttaaac agtacacggg
    47941 ttaatacaca gccggataat aagcttgcgg cgttgttatg cgcgcggata aatggcagtc
    48001 ctgcttgttt tgccgcctgt atggattcat caaatgaata taaaaaaatc agcccccttc
    48061 tgcgcaaggg cgaaaatgaa attaaccaat gggctgaccg tcattctgtt gagcacgcta
    48121 ccgttcaggc gatacaatgg ctgacccgag cacccgatcg ctttagcgcc gcccagttca
    48181 gccctttact cgaacacgaa aaatcatcaa cgcagattat taatctgctg gtatggagcg
    48241 ggctgtgtgg ctggataaat cgcttaaaaa tcgcgttggg tgagacatat taaccttgcc
    48301 gcgtcagaca gattcgcgta aaactgtcag ccgctctaat ggccaccaaa atagacaatt
    48361 atgtttcagg acaacccgct gctagcgcag cttaaacagc aactgcattc ccagacgcca
    48421 cgcgctgaag gggtggtaaa agccacagaa aaaggctttg gcttcctgga agtcgacgcg
    48481 caaaaaagtt atttcattcc gccgccgcag atgaaaaaag tcatgcatgg cgaccgaatt
    48541 atcgcggtga tccacagtga aaaagaacgt gaatccgcag agccagaaga actggttgaa
    48601 ccgttcctga ctcgtttcgt gggtaaagtt cagggcaaaa atgaccgtct ggccatcgtt
    48661 cctgatcatc cactcttaaa agacgccatt ccttgccgcg cagcccgtgg cctgaaccac
    48721 gagtttaaag aaggcgactg ggcggttgcc gaaatgcgcc gtcatccgct gaaaggcgat
    48781 cgttctttct atgctgaact gacacaatac atcacttttg gtgacgacca ctttgtaccg
    48841 tggtgggtta cccttgcgcg ccataatctg gaaaaagaag caccagacgg cgtcgctacc
    48901 gcaatgctcg atgaaggtct ggttcgtgaa gatctgaccg cgctggattt tgtcaccatc
    48961 gacagtgcca gcacagaaga tatggatgac gcccttttcg ctaaggcgtt gccggatgac
    49021 aaacttcagc tgattgtggc gattgccgat ccaaccgcgt ggattgctga aggcagcaag
    49081 ctggacaaag ccgcgaaaat tcgcgcattc accaactatc tgcctggctt caacatccct
    49141 atgctgcctc gcgagctttc tgacgatctc tgctcactgc gcgccaatga agtccgcccg
    49201 gtactggcat gccgcatgac gctctccgct gatggcacca ttgaagataa tatcgaattc
    49261 tttgccgcca ccatcgaatc caaagcaaag ctggtgtatg accaggtttc tgactggctg
    49321 gaaaataccg gtgactggca gcctgaaagt gaagcaattg ccgaacaagt ccgtttgcta
    49381 gcgcaaattt gccaacgccg cggcgagtgg cgtcataacc acgcactggt gtttaaagat
    49441 cgcccggatt accgctttat tctcggtgaa aaaggtgaag tgctggatat cgtcgccgag
    49501 cctcgtcgca ttgccaaccg tatcgtcgaa gaagcgatga ttgccgctaa catttgtgcg
    49561 gcccgcgtac tgcgcgataa gctcggtttt ggcatctata acgtgcatat gggctttgat
    49621 ccggcgaatg ccgacgcgct ggcagcgttg ctgaaaacgc acggtctgca tgtcgatgcc
    49681 gaagaagtgc tcacgctgga cggtttctgc aaactgcgtc gtgaactgga cgcgcaacca
    49741 actggtttcc tcgacagccg cattcgtcgc ttccagtcat ttgctgaaat tagcactgaa
    49801 cccggtcctc actttggcct cggtctggaa gcatacgcca cctggacttc gccgatccgt
    49861 aaatatggcg acatgatcaa ccaccgtctg ctgaaagcgg ttatcaaagg cgaaactgcg
    49921 acgcgcccac aggatgaaat cactgtccaa atggccgagc gtcgccgtct caaccggatg
    49981 gcagaacgtg atgttggtga ctggttatac gcacgcttcc tgaaagacaa agccgggacc
    50041 gacacccgtt tcgcagcgga aattgtcgat atcagccgtg gcggcatgcg tgttcgtttg
    50101 gttgataacg gcgctatcgc ctttattccg gcacctttct tacacgctat gcgcgatgaa
    50161 ctggtttgca gccaggaaaa cggcactgta caaattaaag gtgaaacggt ttacaaagtg
    50221 actgacgtta ttgacgtcac tattgccgaa gtccgcatgg aaacccgcag cattattgcg
    50281 cgcccggtcg cgtaatctcc tttcacggcc cattcctcat ggatgggccg tttatttccc
    50341 cgctctacct tcgttatatc caagcagatt ttttaactga ctttcgtttg aaaactggcg
    50401 tttttccaaa actggattac aaataaatat ataaaaaaaa caatgcgttt agccgccgtt
    50461 aatttcatgt aagtcatgtt gttcctttta tttaaccgcg gactccgcag ttaaccggag
    50521 gatatgcatc atgaaaaccg ttagggagtc cacaacgttg tacaactttc tcggatcgca
    50581 caatccatac tggcggttga cggaaagcag cgatgttttg cgcttttcta ccaccgaaac
    50641 cacagaacct gatcgtacat tgcagttatc tgccgaacag gctgctcgca tcagggaaat
    50701 gacggtcatc acctccagcc tgatgatgag tctgaccgtc gatgaaagcg atctttctgt
    50761 gcatctggta ggacgaaaaa tcaataaacg ggaatgggct ggcaacgcgt ctgcatggca
    50821 tgacacaccg gcggttgctc gtgatttatc acacgggctt tcctttgctg agcaggtagt
    50881 ttctgaagca cattccgcaa tagtgattct cgacagccgg gggaatatcc aacgcttcaa
    50941 tcggttatgt gaagattaca cagggttgaa agaacacgac gtcattgggc aaagcgtgtt
    51001 taaactgttt atgagccgtc gtgaagctgc ggcatccagg cgcaataacc gtgtattttt
    51061 tcgaagcggc aatgcatatg aagtcgaact gtggatacca acatgtaaag gccagcggct
    51121 gtttctgttt cgcaataaat ttgtccacag cggcagtggc aaaaacgaga tttttttaat
    51181 ctgttccggc accgacatta ccgaagagcg ccgcgctcag gagcgactgc gtattctggc
    51241 aaataccgac agtatcaccg gactgccgaa tcgtaacgca atgcaggatt taatcgatca
    51301 cgctattaat catgcagata acaataaagt tggggttgtg tatcttgatt tggataattt
    51361 caaaaaggtc aacgacgcct atgggcattt gtttggtgac cagttattac gcgacgtgtc
    51421 attggctatt ttaagctgtc tcgaacatga ccaggtgttg gcgcgtccag gtggggatga
    51481 gtttctggta ctggcatcca acacctcaca aagcgcgctg gaagcaatgg catcacgaat
    51541 tttgacccgc ttacggctcc cctttcgcat tggtttaatt gaagtttata ccagctgttc
    51601 agtaggtatt gcactctctc ccgaacatgg ttcagacagc acggctatta ttcgtcacgc
    51661 cgacacagca atgtacacag cgaaggaagg cggacgagga caattttgcg tttttacccc
    51721 agaaatgaat caacgggtat ttgaatatct ctggctggat accaacttgc gtaaagcact
    51781 ggaaaacgat cagttggtta ttcactatca accgaaaatc acctggcgtg gcgaagtgcg
    51841 cagtctggaa gcactagtac gttggcagtc acctgaacgt gggttgattc caccgttgga
    51901 cttcatttcc tacgccgaag agtcagggct aattgtgcct ttaggccgtt gggtgattct
    51961 cgatgtcgta cgccaggtgg caaagtggcg ggaaaaaggc ataaacctgc gagtggcggt
    52021 aaatatttct gcacgtcagc tcgccgatca aaccattttc accgccctga aacaggttct
    52081 ccaggaactc aattttgaat actgccctat agatgttgaa ctgacagaga gttgtctgat
    52141 tgagaatgat gaactggcac tgtctgttat tcaacaattt agccaactag gtgcgcaagt
    52201 gcatctggat gattttggta ccggctactc ttcactttcg caactggcgc gctttccgat
    52261 cgatgccatc aaacttgacc aggtttttgt tcgagatatt cacaaacaac ctgtctcgca
    52321 gtcactggtc cgggcgatcg tcgctgtggc ccaggcattg aatcttcagg tgatcgccga
    52381 aggtgtagag agtgcaaagg aagatgcttt tttaaccaag aacgggatca atgagcggca
    52441 aggatttttg tttgccaaac cgatgcccgc cgtcgccttc gaacgctggt ataaacgcta
    52501 tctgaagcgc gcataagtag atgaatatat atacttagcg ctatatcccg tcattatata
    52561 tcgtattcct ggatgataat tagccgttgc tgttaaacac ttctctttta gcggcaattt
    52621 ttgcattatt cattttcccc cttttgctat aaggatgtcc ttgatgtctg aatttgatgc
    52681 ccagcgcgtt gccgaacgta ttgatattgt gttagatatc cttgtggcag gcgattacca
    52741 ctctgcgatc cataatctcg agatacttaa agcagaactc ttgcgccagg ttgctgaatc
    52801 tacgccagac atcccgaagg caccgtggga gatttaacaa tcgtgccttc aggttttcct
    52861 atttgaggat gcccggcgcg tcagt
//
LOCUS       DAZGLJ010000007        49729 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000007 DAZGLJ010000000
VERSION     DAZGLJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.007"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(64..1308)
                     /gene="mtr"
                     /locus_tag="ACHK49_000384"
     CDS             complement(64..1308)
                     /gene="mtr"
                     /locus_tag="ACHK49_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO9261082.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(1462..3351)
                     /gene="deaD"
                     /locus_tag="ACHK49_000385"
     CDS             complement(1462..3351)
                     /gene="deaD"
                     /locus_tag="ACHK49_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO9261083.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEEQDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(3344..3424)
                     /gene="yrbN"
                     /locus_tag="ACHK49_000386"
     CDS             complement(3344..3424)
                     /gene="yrbN"
                     /locus_tag="ACHK49_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO9261084.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(3531..4415)
                     /gene="nlpI"
                     /locus_tag="ACHK49_000387"
     CDS             complement(3531..4415)
                     /gene="nlpI"
                     /locus_tag="ACHK49_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO9261085.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(4524..6659)
                     /gene="pnp"
                     /locus_tag="ACHK49_000388"
     CDS             complement(4524..6659)
                     /gene="pnp"
                     /locus_tag="ACHK49_000388"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO9261086.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(6906..7175)
                     /gene="rpsO"
                     /locus_tag="ACHK49_000389"
     CDS             complement(6906..7175)
                     /gene="rpsO"
                     /locus_tag="ACHK49_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO9261087.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(7324..8268)
                     /gene="truB"
                     /locus_tag="ACHK49_000390"
     CDS             complement(7324..8268)
                     /gene="truB"
                     /locus_tag="ACHK49_000390"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO9261088.1"
                     /translation="MSRPRRRGRDINGVLLLDKSQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(8268..8669)
                     /gene="rbfA"
                     /locus_tag="ACHK49_000391"
     CDS             complement(8268..8669)
                     /gene="rbfA"
                     /locus_tag="ACHK49_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO9261089.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(8833..11505)
                     /gene="infB"
                     /locus_tag="ACHK49_000392"
     CDS             complement(8833..11505)
                     /gene="infB"
                     /locus_tag="ACHK49_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO9261090.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(11530..13017)
                     /gene="nusA"
                     /locus_tag="ACHK49_000393"
     CDS             complement(11530..13017)
                     /gene="nusA"
                     /locus_tag="ACHK49_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO9261091.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(13045..13497)
                     /gene="rimP"
                     /locus_tag="ACHK49_000394"
     CDS             complement(13045..13497)
                     /gene="rimP"
                     /locus_tag="ACHK49_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO9261092.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(13704..13780)
                     /locus_tag="ACHK49_000395"
     tRNA            complement(13704..13780)
                     /locus_tag="ACHK49_000395"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13744..13746),aa:Met,seq:cat)
     gene            14128..15471
                     /gene="argG"
                     /locus_tag="ACHK49_000396"
     CDS             14128..15471
                     /gene="argG"
                     /locus_tag="ACHK49_000396"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO9261093.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(15479..17104)
                     /gene="yhbX"
                     /locus_tag="ACHK49_000397"
     CDS             complement(15479..17104)
                     /gene="yhbX"
                     /locus_tag="ACHK49_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO9261094.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(17563..17649)
                     /locus_tag="ACHK49_000398"
     tRNA            complement(17563..17649)
                     /locus_tag="ACHK49_000398"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17613..17615),aa:Leu,seq:gag)
     gene            complement(17664..17996)
                     /gene="secG"
                     /locus_tag="ACHK49_000399"
     CDS             complement(17664..17996)
                     /gene="secG"
                     /locus_tag="ACHK49_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO9261095.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(18224..19561)
                     /gene="glmM"
                     /locus_tag="ACHK49_000400"
     CDS             complement(18224..19561)
                     /gene="glmM"
                     /locus_tag="ACHK49_000400"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO9261096.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(19554..20402)
                     /gene="folP"
                     /locus_tag="ACHK49_000401"
     CDS             complement(19554..20402)
                     /gene="folP"
                     /locus_tag="ACHK49_000401"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO9261097.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(20492..22426)
                     /gene="ftsH"
                     /locus_tag="ACHK49_000402"
     CDS             complement(20492..22426)
                     /gene="ftsH"
                     /locus_tag="ACHK49_000402"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO9261098.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(22526..23155)
                     /gene="rlmE"
                     /locus_tag="ACHK49_000403"
     CDS             complement(22526..23155)
                     /gene="rlmE"
                     /locus_tag="ACHK49_000403"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO9261099.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            23281..23574
                     /gene="yhbY"
                     /locus_tag="ACHK49_000404"
     CDS             23281..23574
                     /gene="yhbY"
                     /locus_tag="ACHK49_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO9261100.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(23730..24206)
                     /gene="greA"
                     /locus_tag="ACHK49_000405"
     CDS             complement(23730..24206)
                     /gene="greA"
                     /locus_tag="ACHK49_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO9261101.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            24454..25887
                     /gene="dacB"
                     /locus_tag="ACHK49_000406"
     CDS             24454..25887
                     /gene="dacB"
                     /locus_tag="ACHK49_000406"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO9261102.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(25927..27099)
                     /gene="cgtA"
                     /locus_tag="ACHK49_000407"
     CDS             complement(25927..27099)
                     /gene="cgtA"
                     /locus_tag="ACHK49_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO9261103.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRIIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(27115..28080)
                     /gene="yhbE"
                     /locus_tag="ACHK49_000408"
     CDS             complement(27115..28080)
                     /gene="yhbE"
                     /locus_tag="ACHK49_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO9261104.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYALCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(28207..28464)
                     /gene="rpmA"
                     /locus_tag="ACHK49_000409"
     CDS             complement(28207..28464)
                     /gene="rpmA"
                     /locus_tag="ACHK49_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO9261105.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(28485..28796)
                     /gene="rplU"
                     /locus_tag="ACHK49_000410"
     CDS             complement(28485..28796)
                     /gene="rplU"
                     /locus_tag="ACHK49_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO9261106.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            29055..30026
                     /gene="ispB"
                     /locus_tag="ACHK49_000411"
     CDS             29055..30026
                     /gene="ispB"
                     /locus_tag="ACHK49_000411"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO9261107.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            30253..30531
                     /gene="sfsB"
                     /locus_tag="ACHK49_000412"
     CDS             30253..30531
                     /gene="sfsB"
                     /locus_tag="ACHK49_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO9261108.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(30579..31838)
                     /gene="murA"
                     /locus_tag="ACHK49_000413"
     CDS             complement(30579..31838)
                     /gene="murA"
                     /locus_tag="ACHK49_000413"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO9261109.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(31893..32147)
                     /gene="ibaG"
                     /locus_tag="ACHK49_000414"
     CDS             complement(31893..32147)
                     /gene="ibaG"
                     /locus_tag="ACHK49_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO9261110.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(32307..32600)
                     /gene="mlaB"
                     /locus_tag="ACHK49_000415"
     CDS             complement(32307..32600)
                     /gene="mlaB"
                     /locus_tag="ACHK49_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO9261111.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(32600..33235)
                     /gene="mlaC"
                     /locus_tag="ACHK49_000416"
     CDS             complement(32600..33235)
                     /gene="mlaC"
                     /locus_tag="ACHK49_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO9261112.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(33254..33805)
                     /gene="mlaD"
                     /locus_tag="ACHK49_000417"
     CDS             complement(33254..33805)
                     /gene="mlaD"
                     /locus_tag="ACHK49_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO9261113.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(33810..34592)
                     /gene="mlaE"
                     /locus_tag="ACHK49_000418"
     CDS             complement(33810..34592)
                     /gene="mlaE"
                     /locus_tag="ACHK49_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO9261114.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(34600..35409)
                     /gene="mlaF"
                     /locus_tag="ACHK49_000419"
     CDS             complement(34600..35409)
                     /gene="mlaF"
                     /locus_tag="ACHK49_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO9261115.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            35619..36596
                     /gene="yrbG"
                     /locus_tag="ACHK49_000420"
     CDS             35619..36596
                     /gene="yrbG"
                     /locus_tag="ACHK49_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO9261116.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            36610..37596
                     /gene="kdsD"
                     /locus_tag="ACHK49_000421"
     CDS             36610..37596
                     /gene="kdsD"
                     /locus_tag="ACHK49_000421"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO9261117.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            37617..38183
                     /gene="kdsC"
                     /locus_tag="ACHK49_000422"
     CDS             37617..38183
                     /gene="kdsC"
                     /locus_tag="ACHK49_000422"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO9261118.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCVLTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            38180..38755
                     /gene="lptC"
                     /locus_tag="ACHK49_000423"
     CDS             38180..38755
                     /gene="lptC"
                     /locus_tag="ACHK49_000423"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO9261119.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            38724..39281
                     /gene="lptA"
                     /locus_tag="ACHK49_000424"
     CDS             38724..39281
                     /gene="lptA"
                     /locus_tag="ACHK49_000424"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO9261120.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            39288..40013
                     /gene="lptB"
                     /locus_tag="ACHK49_000425"
     CDS             39288..40013
                     /gene="lptB"
                     /locus_tag="ACHK49_000425"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO9261121.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            40061..41494
                     /gene="rpoN"
                     /locus_tag="ACHK49_000426"
     CDS             40061..41494
                     /gene="rpoN"
                     /locus_tag="ACHK49_000426"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO9261122.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            41517..41804
                     /gene="hpf"
                     /locus_tag="ACHK49_000427"
     CDS             41517..41804
                     /gene="hpf"
                     /locus_tag="ACHK49_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO9261123.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            41922..42413
                     /gene="ptsN"
                     /locus_tag="ACHK49_000428"
     CDS             41922..42413
                     /gene="ptsN"
                     /locus_tag="ACHK49_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO9261124.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            42459..43313
                     /gene="rapZ"
                     /locus_tag="ACHK49_000429"
     CDS             42459..43313
                     /gene="rapZ"
                     /locus_tag="ACHK49_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO9261125.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            43310..43582
                     /gene="npr"
                     /locus_tag="ACHK49_000430"
     CDS             43310..43582
                     /gene="npr"
                     /locus_tag="ACHK49_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO9261126.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            43796..44428
                     /gene="yrbL"
                     /locus_tag="ACHK49_000431"
     CDS             43796..44428
                     /gene="yrbL"
                     /locus_tag="ACHK49_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO9261127.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(44425..45153)
                     /gene="mtgA"
                     /locus_tag="ACHK49_000432"
     CDS             complement(44425..45153)
                     /gene="mtgA"
                     /locus_tag="ACHK49_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO9261128.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(45150..45803)
                     /gene="elbB"
                     /locus_tag="ACHK49_000433"
     CDS             complement(45150..45803)
                     /gene="elbB"
                     /locus_tag="ACHK49_000433"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO9261129.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(46033..48369)
                     /gene="arcB"
                     /locus_tag="ACHK49_000434"
     CDS             complement(46033..48369)
                     /gene="arcB"
                     /locus_tag="ACHK49_000434"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO9261130.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(48465..49394)
                     /gene="yhcC"
                     /locus_tag="ACHK49_000435"
     CDS             complement(48465..49394)
                     /gene="yhcC"
                     /locus_tag="ACHK49_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO9261131.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
BASE COUNT        12246 a        13479 c        11993 g        12011 t
ORIGIN      
        1 ctgataagcg aagcgcatca ggcattgtgt aggcagcaga aatgtcggat aaggcaccgc
       61 tgattactga tacaccggca gtaaattaaa gctcgataaa atatggacca gtgcgttgcc
      121 aacgccaaac accagaatca gcgtaatcat cggcttgccg ccccagacgc ggaatttcgg
      181 gctgccaaag cgtttacgcg atgcgcgggc taacagcgcc ggaacaattg ccgcccagat
      241 ggtagctgct aaaccagcat aaccaatggc gtacaggaat ccgttcggga acagtagacc
      301 acccacaaca ggcggggcaa aggtcagcaa tgccgttttc aagcgcccca gggctgagtc
      361 atcgaaacca aacagatctg ccagatagtc aaacaaaccc agcgttacgc caaggaacga
      421 actcgctacc gcaaagtttg agaacacgac cagcagaagg tccaggctac ggctgttcag
      481 cacgccgctt aatgcctgta ccagcacatc aatattaccg cccttctctg caataccgat
      541 aaactccgga cgcgggatgt tacccatcgt cgccagcaac cagatggtat acagcgccag
      601 tgccatcagc gtaccgtaca ccaggcattt cacgatggtt ttcggatctt tgccgtaata
      661 cttcatcagg cttggcacgt taccgtgata accaaacgac gccaggcaga acggcaatgt
      721 catcagcaga tacggcgcat aagatgcatt gctttctgca acgttgaaca atgtcgcagg
      781 ctgcacatgc cccaacaggc tgccaaaggt gaggaagaag gtaatgactt tcgcccccag
      841 cacaatcgcc gtcatgcggc tgacggcctt agtactcaac cacaccacaa acgctaccag
      901 cagtgcaaaa ccaaaacccg ccgcccgtgc cgggacgttt agcgacatct ctgcgaaggt
      961 gtgatgcaga attgaaccac tggcagaaat ataggcatag gtcaggatat agagcacaaa
     1021 ggcaatggaa ataccgttga ccacgttcca gcctttgccc agcaaatctt tggtgatggt
     1081 gtcaaaactc gaaccgattc ggtaattcag gttagcttcc agaatcatca agccggaatg
     1141 cagcatacag aaccaggtaa agatcagcgc cgccattgac cagaaaaacc acgccccgga
     1201 catgaccact ggcagagaaa acatccctgc gccaataatg gtgccgccga taatcaccac
     1261 gccgccaagc agcgacggtg acgtttgggt ggtggttagt gttgccatga gggcttctct
     1321 ccagtgaaaa atagtgcgac tgcgttgtta tgcattgcac tgtaccagta cacgagtaca
     1381 aaagacagaa aaaaagcccc gatggtaaaa atcggggctg tatatattat tttacagatt
     1441 gtgttcgctg ttcagcgatg attacgcatc accaccgaaa cgacgacgac cggtagagtc
     1501 atcacgacgc ggagcgcggc cttcacgacg ttcgccgcta aaacgacgac catcaccacg
     1561 gccaccttca cggcgttcac cgctgaagtt acgaccgcct tcacgacgtt cgccaccgaa
     1621 accacgacca ccgccacgac gctcaccgcc agtatgcggc tgcgcatcgc ccagcaactg
     1681 catattcatc ggcttgttca gaatgcgagt acgcgtaaag tgttgcagta cttcacccgg
     1741 catacctttc ggcagttcga tagtggagtg agaagcaaac agcttaatgt taccgatgta
     1801 acggctgctg atgtcgcctt cgttagcaat cgcgccaacg atatgacgaa cttcaacacc
     1861 atcatcgcgg cccacttcaa tgcggtaaag ctgcatgtca ccaacatcac gacgttcacg
     1921 acgcggacga tcttcacggt cgccacgtgg gccacggtcg ttacgatcgc gcggaccacg
     1981 gtcatcacgg tcacggaatt cacgtttcgg acgcatcggc gcatctggcg gaacaatcag
     2041 agtacgttca ccctgtgcca ttttcagcag tgccgcagcc agagtttcga gatcctgctc
     2101 ttcaccttct gcagtcggct gaattttgct cagcagtgcg cggtattgat ccagatcgct
     2161 gctttccagc tgctgctgta ctttagcggc gaatttttcc agacggcgtt tgcccagcag
     2221 ttctgcgttc ggcagttcta cttccggaat agtcagcttc atagtacgtt caatgttgcg
     2281 cagcagacga cgctcgcggt tctcaacgaa cagcagcgcg cggccagcac gacccgcacg
     2341 accggtacga ccgatacggt gaacgtaaga ctcagaatcc atcgggatat cgtagttaac
     2401 taccaggctg atacgctcaa cgtccaggcc acgggctgca acgtcggtcg caatcaggat
     2461 gtccagacga ccatctttca ggcgttccag tgtctgttca cgcagcgcct ggttcatgtc
     2521 accgttcagc gcggcgctgt tgtagccgtt acgctcaaga gcttcagcca cttccagagt
     2581 cgcgtttttg gtacgaacga agataatcgc cgcatcaaaa tcttccgctt ccaggaaacg
     2641 taccagtgct tcgtttttgc gcatacccca gacagtccag tagctctggc tgatgtcagg
     2701 acgggtagtc acgctggact gaatgcgcac ttcctgcggc tctttcataa agcggcgggt
     2761 aatgcgacga atcgcttccg gcatggttgc agagaacaga gcggtctgat gaccttccgg
     2821 gatctgcgcc ataatggttt caacgtcttc gatgaagccc atgcgcagca tttcgtcagc
     2881 ttcatccaga accagaccgc tcagtttaga gaggtccaga gtgccacgtt tcaggtggtc
     2941 cagcagacgg cccggagtac cgacaacgat ctgcggcccc tgacgcaggg cgcgtaattg
     3001 cacgtcataa cgctggccgc cgtatagagc aaccacattt acgccgcgca tgtgtttaga
     3061 gaaatccgtc attgcttcag caacctgtac cgccagttcg cgggtcggtg ccagcaccag
     3121 aatctgtggt gctttcagct caggatcaag attctgcaac agaggtaaag agaatgctgc
     3181 agtttttccg ctccccgtct gggccatacc cagaacgtcg cggccattca gcagatgtgg
     3241 aatacactct gcctgaattg gagatggttt ttcgtaaccc agatcgttaa gggcttcaag
     3301 gataggagcc ttcaggccca gatctgcaaa agtggtttcg aattcagcca tgtagtacgt
     3361 gtgcctcaaa attaatggcg gccagtctac ataactcatc atgaaattga tcagcaattt
     3421 tcattgaaaa gtgtgaaccg gctcaaagta ggtgtattaa cgaacaacaa cgccctcacc
     3481 cgttaaggtg atggcaatca aaaaagatta cgggctgatg tgtacgtcag ctattgctgg
     3541 tccgattctg ccaggtcatc ttggtcctgg cccaggagcg ataattccaa caatgcgtat
     3601 cggtgctcaa caaagttatg aacgttgttg gcaaccgcca gtttgaacag tgccgtggcg
     3661 ctgtccaaat cccccagact taggtagtac ttacctaaat agaagttggt ttcactgaga
     3721 tgctcagcga gcgaggtgtt atccgttgcg tccgccttga gcctttccat taacgtttgt
     3781 tcgctaatgt tgcccaggta gaactcgaca atgttccatc cccactgttc cttatccgat
     3841 ttttcgaagt gctgtttcaa cacttcttta gcctgcttct catcgagctt ctgctcggcg
     3901 agataaagcc acagactacg gaaaggatca ttgggatcgt cttgataaaa cgccagcaga
     3961 tcatcttgcg ctaacttgtc acgaccgccg taatataatg cgatcccgcg attcaagtgc
     4021 gcgtagttgt aagttggatc aagctcaagt acagaatcaa acgcttcata ggcagcatca
     4081 aaattgcctg cctgcgttaa atatatgcct aagtaattga atacttcagg catatccggt
     4141 cggattgcca gcgcttgcga aaaatcgtta cgcgctaatg ccctcagacc gagactatca
     4201 tacaacactc cgcgctcata taaaagctgt gcgcgttcgt catcggttaa agcccgactg
     4261 gcaaggattt gttccatacg tgccagaatc acttcctgct gtaaagtcgg ttgcaatggt
     4321 accgcgagga cttcactttt acgccaggaa gtattactgc atcctgcaag cgtaagtgct
     4381 gtcgcaacga aacaccagcg caaaaaaggc ttcatttccc actcccgaag accacggttg
     4441 aatgaacgtc ctgttcccgg ttgctaacaa ggcgtcctgc ccggttaaaa gccccccgcc
     4501 gcagcggagg gcaaatggca accttactcg ccctgttcag cagccggagc ttccggtgct
     4561 gcagcaggtt gagactgctc agtcgcttct ttaatgctca gacggatacg gccctggcga
     4621 tcaacttcca gaactttcac cggtacttcc tgacccatct gcaggtaatc ggtcactttc
     4681 tcaacgcgtt tgtcagcgat ttgagagatg tggaccagac cttctttacc gccgccgatg
     4741 gcaacaaatg cgccaaagtc aacgatacgg gtcactttac cagtgtagac gcggcccact
     4801 tcgatttctg cagtgatctc ttcgatacga cgaatagcat gtttcgcttt ctcgccgtcg
     4861 gtcgctgcga tcttcacagt accgtcatct tcgatttcga tggtagtgcc agtttcttcg
     4921 gtcagggcac ggataacaga gccgccttta ccgataacgt ctttgatctt gtccgggttg
     4981 atcttgatgg tatggatacg cggtgcgaac tcagagatat cgccacgcgg cgcgttgatc
     5041 gcctgttcca ttacgcccag gatatgcaga cgcgcacctt tagcctggtt cagcgcaacc
     5101 tgcatgatct ctttggtgat accttcaatt ttgatatcca tctgcaatgc agagataccc
     5161 tcgcgggaac ccgcaacttt gaagtccata tcgcccaggt gatcttcgtc gcccaaaatg
     5221 tcagacagta caacgtagtt gtcgccttct ttcaccagac ccattgcgat acccgcaacg
     5281 gcagctttga tcggcacacc tgcgtccatc agcgccagag acgcgccgca cacggaagcc
     5341 atagaagagg aaccgttgga ttcagtgatt tcagacacaa cacgtacggt gtacgggaat
     5401 ttgtccatat ccggcatgac tgccagcacg ccgcgcttcg ccagacgacc gtgaccaatt
     5461 tcacgacgct tcggagaacc gaccatgccg gtttcgccta cggagtacgg agggaagttg
     5521 tagtggaaca ggaaggtatc ggtacgttcg cccatcagtt catcaagaac ctgcgcgtca
     5581 cgagcagtac ccagcgttgc ggtaaccagc gcctgcgttt caccacgggt gaacagcgca
     5641 gaaccgtgag tacgcggcag cacgccagta cgcacatcca gaccacggat catatctttt
     5701 tcacgaccgt cgatacgcgg ttcgcctgcc agtacgcggc tacgaacaac gtttttctcg
     5761 atagcgtgca gaatttcacc cagttcgttt tcgtccaggg tttcgtcttc agcaagcagc
     5821 gtcgcgatgg tttcagattt gatgacatca acctgcgcat aacgctcttg tttgtcggtg
     5881 atgcggtaag catcgctcag gcgagcttca gccagtgcag caacgcgcgc gttcagcgct
     5941 tcgtttaccg gctccggctg ccagtcccaa cgtggtttac cggcttcttt caccagttca
     6001 ttgatgttct gaataacaac ctgctgttgt tcatgaccga acactactgc gcccagcatc
     6061 tggtcttcgc tcagcagttc agcttcagat tcaaccatca gaacagcggc ttcagtaccg
     6121 gcaacaacca gatccagttt gctctctttc agctcgtcct gagtcgggtt cagtacgtac
     6181 tggtcattga tgtaacctac gcgagcagca ccaatcggac cattgaacgg aataccagac
     6241 agagacagtg ctgcggaagc accaatcatc gcgacgatat ccgggttaac ttgcgggtta
     6301 acagaaacca cggtggcgat aacctgaact tcgttgacga agccttccgg gaacagcggg
     6361 cgaatcgggc ggtcaatcag acgcgcgatc agggtttcgc cttcgcttgg gcggccttca
     6421 cgacggaaga agctacccgg gatacgacca gcagcgtagg tacgctcctg atagttaacg
     6481 gtcagtggga agaagtcctg acctggtttg gcttttttct ggccaacaac ggtaacaaat
     6541 accgcggtgt catccatgct aaccataaca gcggcagtag cctgacgagc catcatgccg
     6601 gtttccagag tcacggtgtg ttggccgtac tggaatttac gaacgatcgg attaagcaat
     6661 gtaatatcct ttctctttct tagacagtac cttacggcac tggtgttaat acccgatctt
     6721 ctgcgcatcc tcgcgactaa tgacaaccct aacccagctc tatgtgggta aagcctctca
     6781 ttagccgcgc gaacctctgc aacggaagat cattcatagc aacaatacat tagtttccag
     6841 tgaattgctg ccgtcagctt gaaaaaaggg gccactcagg cccccttttc tgaaactcgc
     6901 aagaattagc gacgcagacc caggcgctcg atgagctggg tgtaacgtgc tacgtcttta
     6961 cgtttcaggt agtcgagcag tttacgacgc tgagaaacca tgcgcagcag accacgacgg
     7021 ctgtggtgat cttttttgtg ctctgcaaag tggccctgca ggtggttgat ctgtgcagtc
     7081 agcagtgcta cctgaacttc ggtagaaccg gtgtcgtttg cgtcacgacc aaactcagaa
     7141 acgattttag ctgttgcttc agtacttaga gacattttaa aactccaaag tatatagaat
     7201 gaaaggacgc cgatctctaa ttcagcgatc ccagtgtacg ttacgcaaag tgttaaacaa
     7261 tttacgcgac gttaagcggc agtattctac tcgtagcgac ctgttatcgc aagacggtta
     7321 acattacgcc gggtattcaa ccaccaggcg acgaggcgca acgcggcctt catcgtcaat
     7381 ttcgcccata ccgataaatt tgccgttctc accttctgta acgcgaacca gtccttccag
     7441 cggcgcaccg gatgtacgaa ctgggttacc atttttgaag taaacagaag acgttaacgg
     7501 aagattcacc accgggtagt ccgaagctgg actgtccatt ggcatcagta atggatcaag
     7561 taactccgca gccggaatat cctgctgttc ggcttgttcg accagttcac gcagatgttc
     7621 cagggtcacc atccgttcaa ccggatattt acttaccgcc agacgacgca ggtaaataac
     7681 atgcgcgcca caaccgagtt tttcacccag gtcatcaatg atggtgcgga tataagtgcc
     7741 ttttgagcag tgaatttcca gctccagctc attgccttcg tggcgaataa acagcaattc
     7801 ataaacggta atcgggcgcg cttcacgcgg tacttcaatg ccctgacgcg catattcgta
     7861 cagtttttta ccctgatatt tcagtgctga atacatcgaa gggatctgtt cgatatcgcc
     7921 acggaaagta tccagcgccg ccgccagttg ctctgcgcta aaggttaccg gacgttcttc
     7981 aacgatttgt ccgtcggcat cagaagtatc ggtacgctgt ccaagacgcg caatgacccg
     8041 atagcgtttg tcggagtcca gcagatactg ggaaaacttc gtcgcttccc cgaggcaaat
     8101 tggcaacatg ccagttgcca gcgggtccag cgcaccggta tgcccggcac ggttggcgtt
     8161 ataaatccgt ttcacttttt gcagcgcatc attgctggac ataccctgcg atttatccag
     8221 caacaaaacg ccgttaatgt cgcgaccgcg acgacgagga cgactcatta gtcctccttg
     8281 ctgtcgtccg ggttaacacg acgttcttcg tcatgtttga ccacgctggt caccaggttt
     8341 gacatgcgca tcccttcaac cagagagttg tcgtagaaga aggtcagttc cggcacgata
     8401 cgcaggcgca tcgctttccc cagcaggctg cggatgaaac cagaagcttc ttgcaacgct
     8461 ttgatgcccg ctttaaccgc gtcttcatct ttgtcgttga ggaacgtcac atatactttg
     8521 gcatacgcca ggtcgcgaga catttcgaca ccggaaacgg tggtcatcat gcccaggcga
     8581 ggatctttaa tttcacgctg caggatgaga gcgatctctt tttgcatttc ctgcgctacg
     8641 cgctgcgggc gaccaaattc tttcgccata ataaattctc ctgacaaaaa aggggctgtt
     8701 agcccctttt taaaattaat ttcaggtgga agggctgttc acgttggcct gataagacgc
     8761 gccagcgtca catcaggcaa tccatgccgg atgcggcata aacgccttat cccgcatgga
     8821 accctaaaaa ccttaagcga tggtacgttg gatctcgatg atttcgaata cttcgatcac
     8881 atcgccagtg cggacgtcgt tgtagttctt aacgccgata ccacattcca taccgttacg
     8941 gacttcgtta acgtcatctt tgaagcggcg cagggactcc agctcgcctt cgtagataac
     9001 cacgttgtca cgcagaacgc ggatcgggtt gtgacgttta accacacctt cggtaaccat
     9061 acagcctgcg atggcaccaa atttcggcga tttgaacacg tcacgaactt ccgccagacc
     9121 gataatctgc tgtttcagtt ccggagacag cataccgctc atcgccgctt tcacttcgtc
     9181 aatcaggtta tagatgacgg agtagtaacg cagatccagg ctttccgctt caatcacttt
     9241 acgtgcagag gcatcagcac gtacgttgaa gccaaccagg atggcgttgg acgccgcagc
     9301 cagggtggcg tcggtttcgg tgataccacc tacgccagaa ccgatgatct tcactttaac
     9361 ttcgtcagta gacagtttca gcaaggagtc ggagatcgct tccacagaac cctgtacgtc
     9421 tgccttcagg acgatgttca cttcgtgaac ttcgccttcg gtcatgttgg cgaacatgtt
     9481 ctcgagttta gatttctgct gacgcgccag tttaacttcg cggaatttac cctgacgata
     9541 cagtgcaact tcacgcgctt tcttctcgtc acgtacaacg gtaacttcat cacccgcagc
     9601 cggtacgccg gacaggccga ggatttccac cggaatggac ggacccgctt ccagcacttc
     9661 ctgacccagt tcgttacgca tcgcacgaac acgaccgtat tcgaagccac acagaacgat
     9721 atcgcccttg tgcagagtac cttcacgtac cagaacggta gcaaccggac cacgaccttt
     9781 atcgaggaag gattcgataa ccgcaccgct cgccatacct ttacgtaccg ctttcagctc
     9841 cagaacttcc gcctgcagca ggatagcgtc cagcagttca tcgatgccgg tacccgcttt
     9901 cgcagatacg tgtacgaact ggctttcacc gccccactct tccggcagga tgccatactg
     9961 ggagagttcg ttcttaacac gatccggatc ggcttctggt ttatcaatct tgttcactgc
    10021 aaccaccacc ggtacctgcg ccgctttcgc gtgctggatt gcttcgatgg tctgcggcat
    10081 cacgccgtcg tcggcagcaa caaccaggac tacgatgtcc gttgcctgcg caccacgtgc
    10141 acgcattgaa gtaaacgcgg cgtgccccgg ggtgtccagg aaggtgatca tgccgttttc
    10201 agtttcaacg tggtatgcac caatgtgctg ggtaatgccg cccgcttcgc cagaggcaac
    10261 tttcgttgaa cgaatgtagt ccagcagaga ggttttaccg tggtcaacgt gacccatgat
    10321 ggtcacaacc ggcgcgcgcg gttcagccgc agcacccgtg tcacggtcgc tcattaccgc
    10381 ctcttccagc tcgttttcac gacgcaggat aactttgtgg cccatctctt cagcaaccag
    10441 ctgtgcggtt tcctgatcga taacctggtt gatggttgcc attgcgccca gtttcatcat
    10501 cgctttgatg acctgagagc ctttaaccgc catcttgttc gccagttcgc caacggtgat
    10561 ggtttcgccg atcacaacgt cacggttaac ggcctgagct ggcttctgga agccttgctg
    10621 cagggaagaa cctttacgtt ttccgccttt accgccacgt actgctgcgc gtgcttcttc
    10681 acgatcagct tttgattcag cgtgtttgtt gcctttcttc ggacgcgctg ctttcgcgtt
    10741 acgaccacgg ccacgaccgc cttcgacttc acgatcgctt tcgtcttctg cctggcgagc
    10801 atgttgagaa gtagtgacgt gataatcgct ggaatcttca gtcggttccg cgttatcagt
    10861 ccatttgttt tcttccgcca tacgacgtgc ttcttcagca acgcgacgtg cttcttcttc
    10921 gagtttacga cgcgcttctt cttcagcttt acgcttgagc tctgcagctt cctgctcacg
    10981 gcgggctttt tcagcctggg cgtttttagt catatcgtct tgttgattgc tcactttgtc
    11041 tttttccgca gcttcacgtt tcgcttgttc agcagcttca cgcttagctt gttctgcggc
    11101 ctcacgttca gctttttgtt gcgcctcgcg tttagccgat tcttctgcct cacgacgggc
    11161 ttgctcttcc gcttcacgct gcgcttgctc ttccgctgca aggcgttcag cctcttgcgg
    11221 atcgcgtttc acaaaggtgc gtttcttgcg gacttcgatt tgtaccgatt tgctttttcc
    11281 accggtacca ggaatgttaa gggtgctgcg tgttttacgt tgcagcgtca atttgtccgg
    11341 gcctgaattt ttctgattca ggtggtcaat caaagtctgt ttctcttgtg cagacacaga
    11401 gtcgtcagca gacttccgga tacctgcatc agcaaattgc tgtaccaggc gttccacgga
    11461 ggtctgtcgc tctgcggcca gcgttttaat cgttacatct gtcatgctgt tccttcctgc
    11521 tacagtttat tacgcttcgt caccgaacca gcaaatatta cgggcagcca taatcagtgc
    11581 tccggctttt tcgtcggtca acccttcgat atcagccaga tcatcaatgc cctgttcggc
    11641 gagatcttcc agcgtacaaa cgccacgggc ggccagtttg aatgccaaat cacgatctat
    11701 cccttcaagg ttcagcagat cgtcagccgg tttgttatca ccgaggcttt cttcctgggc
    11761 ctgtgcaatg gtggccagtg catttttagc acgctcgcgc agtgcttcaa cggtcggctc
    11821 atcaaggcct tcgatttcca acagctcttt catcggcaca taggccagtt cttccagcgt
    11881 cgagaagcct tcttctacca gaacagtcgc gaagtcttcg tcgatgtcga gatatttggt
    11941 gaaggtgtcg atcgctgcgt gcgcttccgc ctgatgctta gcctgcaggt cgtcaacggt
    12001 catcacgttg agttcccaac cgctcagctg cgaagccaga cgcacgttct gaccgttacg
    12061 gccaatcgcc tgcgccaggt taccggcttc aacggcgata tccatggtgt gtttatcttc
    12121 atccaccacg atagaagcaa cgtctgccgg tgccattgcg ttaatcacaa actgcgccgg
    12181 gttatcatcc cacaggacga tatcgatacg ctcgccgccc agttcagtag aaaccgcctg
    12241 aacacgcgcg ccacgcatac ctacacaggc acctaccgga tcgatacgtt tatcgttggt
    12301 tttcaccgcg attttcgcac gagaacccgg atcgcgagcc gctgctttaa tttcaatcac
    12361 ttcttcgccg atttctggca cttcaatacg gaacagttcg atcagcattt ccggcttgga
    12421 acgagtgacg aacagttgcg cgccacgcgc ttccgggcga acggaataga gcacgccacg
    12481 aacgcggtcg ccagggcgga agttttcacg cggcagcata tcttcgcgca ggatcacggc
    12541 ttcagcattg ttgcccagat ccagagagat gttgtcgcgg tttacttttt tcaccacgcc
    12601 ggtgatgatt tcaccttcgt gttcacggaa ctgatcaacc accatcgcac gttcggcttc
    12661 acgcactttc tgcacgataa cctgttttgc cgtctgggta gtgatacggt caaaggtaac
    12721 agactcaatc tgatcttcaa cgtaatcgcc caggttcagg ctttcatctt cataacgtgc
    12781 ggcttcaagg gtgatttcct tggtcggctg ggtgacttca tcaacaacta accagcgacg
    12841 gaaggtgtca aaatcaccgc ttttgcgatc gatctgtacg cggacgtcga tctcttgttc
    12901 atattttttc tttgttgctg tcgccagcgc gctttccaat gcttcgaaaa tcttctcgcg
    12961 aggtagcgcc ttttcattgg atacggcttc aactacagcc aaaatttctt tgttcatcgc
    13021 gggcttttca cctcatccag actattaaaa gtggggaacc aggttcgcct tctggatatt
    13081 actcagcgcg aacacttcat ctttaccttc gacggtaact gtgatcattt caccgtctac
    13141 cgctttgata acgccctgcc atttacgacg gttttgtacc gccatacgga gaaccagagt
    13201 cacctcttct ccgacaaaac gggcgtagtg ttcagccgtg aacagtgggc gatcgagacc
    13261 cggtgaggag acttccaggt tataagcaac ggtgatggga tcttcaacat ccagcacagc
    13321 acttacctgg tggctcacat cagcacaatc atcaacattg atgccatctt cactatcaat
    13381 atagatgcgc agtgtggatg tgcgaccgcg aataaattcg atgccaacca gttcaaaacc
    13441 cagggcctca actggcgcag taatcatctc tgttaatttt tgctctaatg tggacaagcc
    13501 cacccccaag acataaaaaa agggcctaaa gcccagttat tctgtagtca gataacaaaa
    13561 aaccccgata aatcggggct ttatataact gaaccctata accgcaactg cggtctggag
    13621 cactttccag aaggattttt tcaaatccca ctacgaaggc cgaagtcttc acagtatatt
    13681 tgaaaaagga ctctaaggga aagtggttgc gggggccgga tttgaaccga cgaccttcgg
    13741 gttatgagcc cgacgagcta ccaggctgct ccaccccgcg cctgaaacgt ggcaaattct
    13801 actcgttttg ggtaaaagat gcaaatactg ctgggatttg gtgtaccgag acgggacgta
    13861 aaatctgcag gcattatagt gatccatgcc acattttgtc aacgtttatt gctaataacg
    13921 tgaatgaata tccagttcac tttcatttgt tgaatacttt tgccttctcc tgctctccct
    13981 taagcgcatt attttacaaa aaacacacta aactcttcct gtctccgata aaagatgatt
    14041 aaatgaaaac tcatttattt tgcataaaaa ttcagtgaga gcggaaatcc aggctcatca
    14101 tcagttaatt aagcagggtg ttattttatg acgacgattc tcaagcatct cccggtaggt
    14161 caacgtattg gtatcgcttt ttccggcggt ctggacacca gtgccgcact gctgtggatg
    14221 cgacaaaagg gagcggttcc ttatgcatat actgcaaacc tgggccagcc agacgaagag
    14281 gattatgatg cgatccctcg tcgtgccatg gaatacggcg cggagaacgc acgtctgatc
    14341 gactgccgca aacaactggt ggccgaaggt attgccgcta ttcagtgtgg cgcatttcat
    14401 aacaccaccg gcggcctgac ctatttcaac acgacgccgc tgggccgcgc cgtgactggt
    14461 accatgctgg ttgctgcgat gaaagaagat ggcgtgaata tctggggtga cggtagcacc
    14521 tataaaggaa acgatatcga acgtttctat cgttatggtc tgctgaccaa tgctgaactg
    14581 cagatttaca aaccgtggct tgatactgac tttattgatg aactgggcgg ccgtcatgag
    14641 atgtctgaat ttatgattgc ctgcggtttc gactacaaaa tgtctgtcga aaaagcctac
    14701 tccacagact ccaacatgct tggtgcaacg catgaagcga aggatctgga atacctcaac
    14761 tccagcgtca aaatcgtcaa cccgattatg ggcgtgaaat tctgggatga gagcgtgaag
    14821 atcccggcag aagaagtcac agtacgcttt gagcaaggtc atccggtggc gctgaacggt
    14881 aaaaccttta gcgacgacgt agaaatgatg ctggaagcta accgcatcgg cggtcgtcac
    14941 ggcctgggca tgagcgacca gattgagaac cgtatcatcg aagcgaaaag ccgtggtatt
    15001 tacgaagctc cggggatggc actgctgcac attgcgtatg aacgtctgtt gaccggtatt
    15061 cacaacgaag acaccattga gcagtatcac gcgcatggtc gtcagttggg ccgtctgctg
    15121 taccaggggc gttggtttga ctcccaggcg ctgatgctgc gtgactctct gcaacgctgg
    15181 gttgccagcc agatcactgg tgaagttacc ctggagctgc gccgtgggaa cgattattca
    15241 atcctgaata ccgtctcaga gaacctgacc tacaagccag agcgtctgac gatggaaaaa
    15301 ggcgactcgg tgttctcgcc agatgatcgt attggtcaat tgaccatgcg taatctggat
    15361 atcactgata cccgagagaa actttttggt tatgccaaaa ctggcctgct ctcctcctct
    15421 gccacttcag gcgtgccgca ggtggagaat atggaaaaca aaggccagta attcgacatc
    15481 aaccctgccc ttcaggggtg gggttacctt gcttatcctc tgtaaactcc tttctgagca
    15541 tcacataatc gaaaacatca tggtttgcat ccacaacccg tgagtctcct ttatagtgtg
    15601 caatcacttc ctcaagcgtt tgcggctgtt ccggctttgt tacccccatc cacgcattaa
    15661 taaggtaatt attgtaagct gtcgaaaaga tgttgttttc cgttgtgcga tccacgccat
    15721 ccccaagaac ggggctatac cagataaaca tcgggacatg atatgcctgc tggctagcct
    15781 ccctgcctcc atgaaaatag acgttcttct tcgtagggtc acgttccagg ccgtggtcgg
    15841 caaaatacat gaccgaggcg cggcgatctt ttaataattc aaaaacctga cctagcaaac
    15901 tatctgtgta atgaatggag ttgtcatagc aggcatcctg atcgtcctgc ggttgaaaca
    15961 cggcgctgga ttgcggatag gcgctacaag ccggttcatg gcttccattt aaatgaagaa
    16021 caatcagttt cttttgctgc gtattttgct gtaatgcttg cgataaatgc ggcaacaaca
    16081 attcatcaaa tcctctgaca taaactgttt ccatggcgcg catggcgata ctggtaactg
    16141 ctgtaccatt ctgccgaaaa gcggattgcg agcttagcca gaaagtctga aatcctgctt
    16201 gattagccat attaataatg ttgtcggggt aattatgaat gtcatgactc aaaacagaat
    16261 cagcagttaa agaaagggga accgacagcg cagtgtaagg tgcgccgctt attgcttgat
    16321 taaacagttt gatctgtttt ctttgtgctt caacttgcgg tgtcgtagag cgtgtatatc
    16381 catacaaaga catattgtcg acacgtacag actcccccac aatcaacacg taggtatcaa
    16441 tacctgtatc cctgactgat aattgaaaat acggcaccgt gtttgcaatt gagagtaatc
    16501 tttgatgctc tttcgctgct aaagcaaaat agttgagatt gaaaaacggc gtataggtag
    16561 caaatcgcga cgctagtata tatggactga acgctttttt attttttgca tctttataag
    16621 caaattgaca agcggaaaat aaactgcccg aaatgacaat cagcaataat attcctgtca
    16681 ctttttttgt cggcaaggaa acatcatatt ttattattac tgccaaaaaa agaagggata
    16741 aaaaagcaaa ggcacataga taagggatat acatccccag cattttgaca acttcatccg
    16801 gatcactctg gagcacacta atcgcaaatc catcattgaa agttgtacca aaggtacacc
    16861 atgaatagag gctaatactc atgtctgccg cagtcagtaa cacaaatggt gcggcaacaa
    16921 gcaggcgaag cggaagggat gatatacgtt ttacaaccag gatggtcagg acgaagaaca
    16981 gcaatcgctg aaccatatgc gcgccggaag aggcgactaa gttcgtaata ccaaaaacaa
    17041 taacacaaag atacaacaac caatgagatt taaaacttct agcaaatttg ttgaatactg
    17101 tcatccagaa atccaagttc acaaccaata taaacaatta tcaacaatgt tcaattttat
    17161 atcagacatg tttttcaact ctgatccata tcagaccgaa acactgttag ttttttcttt
    17221 ttaaatgatg cggccattgc gttctcagta attaaataac attatcaatg cgtctctaaa
    17281 cattacgcag aacgtagcca tcagtagtta tactgacagg agcaggatgg tttatcgaga
    17341 agtaaacata tccgtattcg taccggaact ggagttgaca caataaagtg ccaattatgt
    17401 cagcagaagg gaaaagttta taactaaggc gtaccggcac catcgtttca aggtaccagc
    17461 tacgagtaaa gcaactggac gagatacaga tacctgacaa ccattcctca gaccaggacc
    17521 aaaacgaaaa aagacgcttt tcagcgtctc ttttctggaa tttggtaccg aggacgggac
    17581 ttgaacccgt aagccctatt gggcactacc acctcaaggt agcgtgtcta ccaattccac
    17641 cacctcggca cggatactac tttttagttc gggatatcgc tggtcggctt agccggagca
    17701 gctggttgag tttgttcggt tttcaccggt gcactcagat tttcccattc gctaccttta
    17761 ttggttttgt tgctattgat gttacccagc accagactga tgatgaagaa taacgttgcc
    17821 agcagcgccg tcatgcgggt catgaagtta ccagaaccac ttgaaccaaa cagcgtagcg
    17881 gaagcgcctg ctccgaagga ggctcccata tcagcgcctt taccttgctg cagcatgatc
    17941 agaccaacaa ggccaattgc cacaataagg aaaactacta aaagagcttc gtacataatc
    18001 aacctgttcc ttgcggagtt gccgcgtacc aatgcttcaa ccaataaagc gggagttttt
    18061 aatctttccc actgaagcgg gtgtgaatac taaccaaagc gaatgacctt cgcaagggca
    18121 attttatcgc attgtatcaa ctgcggaaaa aaacagcaaa accgatgtgt tcgctgagaa
    18181 aaaaggcgac aatcgccgcc tttttagcca gttatctaac gctttaaacg gcttttactg
    18241 catcggcgat gcggtgtgca aattcagtca cctgcgcttc gtcttcgcct tccaccatca
    18301 cgcgaattaa cggttcggtg ccggatttac gcagcaacac gcgtccacgg ttgcccagcg
    18361 cagcttcaac ctctgcggtc acggctttaa ctgactcatg ctcaagtgga tcgccgctac
    18421 ctgcggtgta acgtacgtta accagaatct gcgggaacat tttcatgccg ctgcaaaggt
    18481 cgtgcaggct catatggtta cgtgccatcg ccgccagcac ctgcaagcca gcaacgatgc
    18541 cgtcaccggt agtagtttta tccagtagga tcacatgacc ggaattctct gcaccgatac
    18601 gccagccttt ctcctgcatt ttttccagta cgtagcggtc gcccactttc gcgcgcgcaa
    18661 atggaattcc taactgtttc agcgccagtt caagccccat gttgctcatc aatgtaccca
    18721 cagcgccacc acgcagctgg ccctgacgaa gaccttcacg cgcgatgata tacatgatct
    18781 gatcgccatc gactttattg ccttcatggt caaccataat cacgcgatcg ccatcgccgt
    18841 cgaaggcaat accgagatcc gctttttcag ccagcacacg agcctggagc gcgcgaacgt
    18901 cggtagcccc cacttcggca ttgatgttta caccgtttgg ctcacaaccg atagcgataa
    18961 cgttcgcccc cagttcgcgc agcacgttcg gcgcgatgtg ataagtcgca ccgtttgcac
    19021 aatccaccac aatcttcagt tcactgaggc taagttcgtt cgggaacgtg gctttgcaaa
    19081 actcgatata gcgacccgcg gcatcaacga tacggctggc tttacccagt tctgccgaat
    19141 caacgcagct gatctccttt tccatttccg cttcgatggc ctcttctacc gcatccggca
    19201 gtttggtgcc gtcgatagag aagaatttaa tgccattatc gtagaacggg ttatgagatg
    19261 cagatatcac aattccggcc tctgcgcgga aggtacgcgt cagataagcc acggccggtg
    19321 ttggcatcgg gccagtgaag agtgcggaaa ggcccgctgc cgccagaccc gcttccagtg
    19381 ctgactccag catatagcca gaaatacgcg tgtctttacc aataataatc ttacgggagc
    19441 cgtggcgcgc cagcacttta cccgcggccc aacccagctt aagcacaaaa tcaggtgtga
    19501 tcggcgcatc ccctacacga ccacgaatcc catcggtacc gaaatattta cgattactca
    19561 tagcgtttgt tttcctttgc agacagagtg gcttccacca cccgcatcgc ttctacggtt
    19621 tctttgacgt catgaacacg aatgatgtgc gcgccttgca ttgcggcaat gaccgcacag
    19681 gccagactac cgctcaggcg ctcggacggc cccacgttca gcagctgccc aatcatcgat
    19741 tttcgtgaca tacccaccaa cagcggcagg ttgaaatggt gaaattcagc caggcgcgcc
    19801 agtaatgaat agttatggga gagattttta ccgaaaccga atccggggtc gagcaacaat
    19861 ttctcttttg cgatacccgc ctgctcgcaa cgtgctattt gctcaataaa gtagcgattc
    19921 acttctgcaa agacatcgtc atacttcgga gcttcctgca tggtttttgg atttccctgc
    19981 atatgcatca gacaaaccgg taaaccggtt tctgcagccg cctccagagc gccaggttcg
    20041 gaaagggagc ggatatcatt aataatgtga gcgccaactt tcgctgactc acggatgact
    20101 tctggtttgg atgtatcgac cgagatccag acttcgaagc gttgagcaat tgcctcaacc
    20161 acaggaataa cacgttgcaa ctcttcttca acgctaactt ccgccgcccc tgggcgcgtg
    20221 gactcgccac caacgtcaat gatcgtcgcg ccagcattga tcatcagatt cgcatgtttc
    20281 accgcatcta tcagcgagtt atgcgtgcca ccatccgaaa aggaatcagg cgtgacgttg
    20341 aggatcccca ttacgtgagg atggctaagg tccagtgaag taccctgggc aaagagtttc
    20401 atgatgttat ccctagtatg aattgataag aaaaaacccc ggagcacgcc ccggggtttt
    20461 cggtacaaat acagtcatct gatgcgggaa cttacttgtc gcctaactgc tctgacatgg
    20521 tgttacccgg gttcggcgta cgcggttcat caaccggacg aggagccttt ggactaccat
    20581 tgtcgccaga attgttagaa gcgcctggtt cttcccagcc cgctggcgga cgtacatcgc
    20641 gacgtgccat caggtcatca atctgcggtg cgtcgatagt ctcatatttc atgagagcat
    20701 ctttcatcgc atgcagaata tccatattgt cggtcagaag ctgacgcgca cgattatagt
    20761 tacgctcaat cagtgctttc acttcctggt cgatgatacg tgcagtttca tcggacatat
    20821 gtttcgcttt cgctacgcta cggccgagga atacttcacc ttcttcttcc gcgtacagca
    20881 gtggacccaa tttctcagag aagccccact gggtcaccat gttacgtgcc agattggtcg
    20941 caactttaat atcgttggac gcaccggtag aaacatgttc cggcccatag atgatctctt
    21001 ctgccagacg accaccgtac agcgtagaaa tctggctttc cagtttctga cggctggcgc
    21061 tgattgcgtc gccttcaggc aagaagaaag tcacacccag cgcacgaccg cgtgggataa
    21121 tcgtcacttt gtgcaccgga tcgtgttccg gcaccaggcg accgataatc gcatgacccg
    21181 cttcgtggta agccgttgat tctttctgcg cttccgtcat caccatggag cgacgttccg
    21241 cacccatcat gattttgtct ttcgctttct cgaactcaac catcgacaca acgcgtttgt
    21301 tgccacgagc agcgaacagt gccgcttcgt tcaccaggtt cgccaggtca gcaccggaga
    21361 aaccaggagt accacgggca atgattgccg cgtcgatatc gggggccaat ggtacgcgac
    21421 gcatgtgaac tttcaggatc tgctcacgac cgcgaacatc tggcaagccg accacaacct
    21481 gacggtcgaa acggccagga cgcagcaggg ccgggtcgag aacgtccgga cggttagtcg
    21541 cggcgataac gatgatacct tcgttacctt cgaagccatc catctcaacc agcatctggt
    21601 tcagagtctg ttcacgttca tcgtgaccac cgcccagacc agcgccacgc tggcggccta
    21661 cggcgtcgat ttcatcgata aagatgatgc acggtgccgc tttcttcgcc tgttcgaaca
    21721 tgtcacgaac acgggatgca cccacaccga cgaacatttc tacgaagtca gaaccggaga
    21781 tagtaaagaa cggaactttc gcttcgcctg caatcgcttt cgccagcaac gttttaccgg
    21841 tacccggagg accgaccatc aggacgcctt tcgggatctt accgccgagt ttctggaagc
    21901 ggctcggctc gcgcagatac tctaccagtt cagcaacttc ttcttttgct tcgtcgcagc
    21961 ccgcaacgtc agcaaaggtc gttttgatct gatcttccgt cagcatgcgc gctttgctct
    22021 taccaaacga catggcacct ttgccaccgc cgccctgcat ttgacgcatg aagaagatcc
    22081 agacaccaat cagcaacagc atcgggaacc aggagatgaa gatagaagcc agcaggcttg
    22141 gttcttcagg cggttcaccg acaaccttga cgttcttggt caacaggtta tccagtaatt
    22201 tcggatcctg aaccggaatg taagtggtat aacggttact atctttcttg gtaacgttga
    22261 tttcacgtcc gttgatacgc gcttcacgaa cctggtcgtt attgacctct tgtaggaagg
    22321 tagagtaatc caccttacgg ccattagact cgctgggccc aaagctctgg aatactgaca
    22381 tcagcacaac ggcaatgacc agccagagta ttaggttttt cgccatgtca ctcaagggat
    22441 taacctctta ttacaactgt gttaaaaaca gcgtcaggat actctatatc cagcatcttt
    22501 caaactttcg tctgaaatct cccggttagg gtttacgccc ggtcgctaca atatacactt
    22561 cccgcgaacg tgcacgagaa gagtccggct tacgaacttt gaccttcgta aacagggagc
    22621 gaatttccct tagatactca tcgaaacctt cgccctggaa caccttcact acaaaactgc
    22681 cacctggcgc taatacatca cgacacattt ctagcgccag ttccaccaga tacatggcac
    22741 gggggatatc caccgccggt gttccgctca tgtttggtgc catatcggac atgacaacct
    22801 ggactttgct gtcgccaacg cgctccagca gtgctttcat caccagttca tcacgaaaat
    22861 cgccctgaag aaagtccaca ccaacgatag gatccatagg taaaagatcg caagcgatga
    22921 tgcggccttt gccgccaatt tgggtgacca catattgtga ccaaccaccc ggagcagcac
    22981 caaggtcgac aaccgtcatt cccggtttaa agagtttgtc actttgctgt atttcatcaa
    23041 gtttaaacca ggcacgggaa cgtaacccct ttttctgtgc ctgttgaaca tatttatcgc
    23101 taaagtgttc ctgaagccag cggctggagc tggcagaacg cttcttacct gtcatttaac
    23161 tttcccatgg ggataactca tcgtaaccaa ttgcgtaaat ttttacgcgc ctatttggtg
    23221 atatatggga gatggcggta gaatgacccg ttttcaatcc caacgtaagc aaaaaatacg
    23281 atgaatctga gtactaaaca aaaacagcac ctgaaaggtc tggcacatcc gctcaagcca
    23341 gttgttctgc ttggcagtaa tggtttgacc gaaggggtgc tggccgagat tgaacaagcg
    23401 ttagagcacc atgaactcat caaggtgaaa atcgccaccg aagatcgcga aactaaaacc
    23461 ttgatcgtgg aagctatcgt gcgcgaaacc ggcgcctgta atgtacaggt catcggtaaa
    23521 acgctggtgc tttatcgccc aactaaagaa cgtaaaatct cgctgccacg ctaagattat
    23581 cctaaagtta cacacatttg ctgtgtaaaa cgaggggttt tccgcaggca ggagagcaaa
    23641 atgccacgct ctgttcgttg ataaaaggcc gcatagcggc ctttttcctt tctttacaat
    23701 acatcaacat cttgagtatt gggtaattct tacaggtatt ccaccttaat tacttcaaat
    23761 tctacttcgc cgcccggcgt tttgatgacc acaacatcat cttcttcttt gccgatcagg
    23821 ccacgagcaa taggcgagtt tacagaaatc aggttttgtt taaagtcagc ttcgtcatcg
    23881 ccaacgatgc gataagtctg ttcttcgtca gaatccagat tcagcaccgt tacggtagca
    23941 ccgaaaataa cgcgcccatt gttgggcatt ttggtgacat caatcacctg cgcgttcgac
    24001 agcttggctt cgatgtcttt aatacggcct tcgcagaaac cctgctgttc acgagctgcg
    24061 tggtattcgg cgttttcttt caggtcgcca tgctcacgcg cttccgcgat agcagcaatg
    24121 atttcaggac ggcgcacaga tttcagaaaa tccagctctt cgcgtaattt ttcagcgccg
    24181 cgtaaggtca tcggaatagc ttgcatttgt tatacctctt gaatattcct gatggggcaa
    24241 gtcttcaccc accccggctg ttcggcctgc ccggcataac tcctcaccgg gaccagaagc
    24301 aaaaaaatac cgacccgggt acaagtccca ggtcagctac aattcacatt ttgatagtca
    24361 ttttaccctg aagttcccga agggtcatcg tttactttat agggcgttgc gccgtagtat
    24421 gacggctcga ttccaggttg ttagcgcgag attatgcgat tttccagatt tatcatcgga
    24481 ttgaccagct gtatagcgtt cagtgttcag gccgcgaatg ttgatgagta cgttactcaa
    24541 ctccccgctg gtgccaacct tgccctgatg gtgcaaaaag tcggcgcgtc ggcccccgct
    24601 attgattacc acagtcagca gatggcgctg cccgccagta cgcagaaagt gattactgcg
    24661 ctggcggcgt tgattcaact cggccccgat tttcgtttca ccacgacgct tgaaaccaaa
    24721 ggcaatgtgg aaaacggcgt acttaagggt gacttagtgg cgcgatttgg tgccgatccg
    24781 acgttaaaac gtcaggatat tcgcaatatg gtcgcgactt tgaaaaaatc tggcgtcaac
    24841 caaatcgatg gcaatgtgtt gatagatacc tccattttcg ccagccacga taaagccccc
    24901 ggctggccat ggaatgacat gacacaatgc tttagcgctc cgcctgccgc tgccatagtt
    24961 gaccgcaact gtttctccgt ctcgctctac agtgccccaa aacctggtga tatggctttt
    25021 atacgcgtgg catcttatta ccccgttacg atgttcagcc aggtacgcac cctcccccga
    25081 ggttctgccg aagcgcaata ctgcgaactg gatgtcgtac caggcgacct gaaccgcttt
    25141 acgctgacgg gatgcctgcc acaacgttct gagccgctcc cgttggcttt tgccgtgcag
    25201 gatggagcca gctatgccgg ggcaattctg aaagatgagt taaaacaggc aggtatcacc
    25261 tggagcggaa cactgctgcg ccagactcag gttaacgaac ctggaacggt agttgccagt
    25321 aaacagtcgg ccccgttgca cgatctgctt aagattatgc tgaaaaagtc ggacaacatg
    25381 atcgccgata cggttttccg catgataggc catgcgcgct tcaatgtgcc tggaacatgg
    25441 cgggccgggt cggacgccgt gcgtcagatc ctgcgccagc aagccggtgt cgatattgga
    25501 aacaccatta ttgccgatgg ttcagggctt tcgcggcata acctgattgc ccccgccacc
    25561 atgatgcagg tgctgcaata cattgcccaa cacgacaatg aacttaactt tatctccatg
    25621 ctgccgcttg cgggctatga cggctctttg cagtaccgtg caggtctgca tcaggcgggc
    25681 gtggatggaa aagtctcagc gaaaaccggt tcgttgcagg gggtatataa cctggcggga
    25741 ttcattacca cagcgagcgg gcaacgaatg gcgtttgtac aatatctttc tggctatgca
    25801 gtagaaccgg cggatcagcg taatcgccgt attccgttag tgcgttttga aagccgtttg
    25861 tataaagata tttatcagaa caattagtca aaaagaaacc ccggcacatg gccggggttt
    25921 tgctgattaa cgcttgtaaa tgaactcaac gccttcttcg tcgtcttcgt cccagtcatc
    25981 atcccagtcc tcatcatcct cttcagcaat ctcttcaagc tgctggcgat gataatcatc
    26041 ccacatgaat tcgactttct ctggctgttt cgcttcttca gcctgcacga ccgggttttc
    26101 aatgataaag gtcatcacat cccagcagag atctttcacg cccagtccac tcgccgcaga
    26161 gatcagataa tatttatctt cccagcccag cgcttcagca atcgctttcg ctttctcttc
    26221 agcttctgcc ttatccagca gatcgatctt attgaacact aaccaacgcg gtttcgccgc
    26281 cagatcctgg ctgtattttt ccagctcgct gataataata cgcgcgtttt caaccggatc
    26341 ggtgccgtca atcggatcga tatcgatgag gtgcaacagg acgcggcaac gttccaggtg
    26401 cttcaggaag cggataccca gacctgcgcc ttccgcagcg ccttcaatca gtcctggaat
    26461 atcggcaaca acgaagctct tttcgttgtc cattcgtacc acacccagac ttggcaccag
    26521 agtggtaaac ggataatccg ccactttcgg tttagccgcc gataccgcgc gaataaaggt
    26581 cgatttaccc gcgtttggca tccccaacat accgacgtca gccagcagca tcagctccag
    26641 cagcagctca cgcttatcac ccggcgtacc gttggttttc tgccgtggtg tacggttaac
    26701 ggacgatttg aaacgggtat tgcccagacc gtgccagccg cccttagcaa ccagcagacg
    26761 ctgaccgtgt ttagtcatat cgcccatggt ttcaccagta ccttggtcga ttatacgcgt
    26821 acctaccggc actttaatcg tcacgtcttt accgcgctta ccggtacaat cgcggcttgc
    26881 gccgttctga ccgcgctctg cacggaaaga tttttcaaaa cgataatcga taagcgtgtt
    26941 caggttttcg tcggcttcca tccatacgtc accaccatca ccaccgtcgc cgccatccgg
    27001 gccgcctttc ggaatatact tttcgcggcg gaagctcacg caaccattac cgccatcacc
    27061 tgcaacgacc agaatcgatg cttcatcaac aaacttcatt ttattctccg taaatcattc
    27121 gcctgcgcgg ggttgcgata ccaccgttgt atgcttacgt aatccgcccc aaatacgatg
    27181 accaatggcg gaatacatcg cgcccgcaac cacgacaaac gcaccgagat aacctaaaag
    27241 gtttaacatc ggtctggcga agaaatcggg ccaggccagt gataaaagat ctgaaaaaaa
    27301 cagcgtaaac agtggggtga gcgtgatgat cgcgctcacc tgcgctgcct gccagcgagc
    27361 catcgcttcc gccagggcgc catatcctac caatgtattc agtccgcaaa aaattaaaca
    27421 tgcgagctgc cagtggctaa gctgcgctat cactccaggc tttgccagag ggaagagcgc
    27481 aattgtacat aaagcgtaca gtaaaaacag gatctgcggt gaggccagcc gacgcaataa
    27541 aaccttttgc gccacgccat aactcaccca aaccgtcgcc gcaccgaccc caaagataac
    27601 tccccaggtg taatcggtga gctttgtaaa tatctcgacc agactggtgt taaaaaacat
    27661 caccaggccg ctcaggagca tcaatgcccc tacaacctga gtgctgcgca ttttctcttt
    27721 caggataaat acgctggcaa ccatcatgcc aactggcgag agttgcccaa tcacctgcga
    27781 agcggtcgga ctcaggtatt gcaaggatga gctgaacagg atgaagttcc caaacagccc
    27841 ggcggtcgcc actgccaaca aaatcaacca gcgtggctta cgaaacacgc gtaatggcgg
    27901 caacctcttc ttcaccgcaa gaatggcacc aaggccaata ctcgccatca agaaacggta
    27961 aaacacgatt gtcggaggtt ccatcacctc cagcacctgc ttcattgcga ttggcaacgc
    28021 cccccagcaa attgctgtgg tgagcgccaa aagaatgcca atgcctgcct gctgcttcat
    28081 accgtattcc ctgcaaagcg catttgcgga ttaacgtcgc accatgcgca tttttaccgg
    28141 ttatcgaatg taaaaagccc cgcaacgtgt tgcggggctt tcatccgtta ccgggacgcg
    28201 aaaaacttat tcagcttcga tgctgataaa tttacggttt ttcgggcctt taacttcgaa
    28261 tttcacttta ccgtctgctt tagcaaacag agtgtggtca cgaccgcaac ctacgttagc
    28321 gccagcgtgg aatttggtac cacgttgacg aacgatgatg ctacccgcca gaacggattc
    28381 gccaccgaaa cgcttaacgc ccaggcgttt agcttctgaa tcgcgaccgt tacgtgtgga
    28441 gccgccagcc tttttatgtg ccatttgaaa tctctcctca ggtcttaggc gctgatgcca
    28501 gtaattttca catcagtgaa ccactgacga tggccctgct gcttacgata gtgtttacga
    28561 cgacgaaact taacgatttt aactttctcg ccacgaccgt gagcaacaac ttcagctttg
    28621 attacgccgc catcaacgaa aggaacgccg attttgactt cttcaccgtt tgcgatcatc
    28681 agcacttcag cgaactcaac agtttcgcca gttgcgatgt ccagcttttc caggcgaacg
    28741 gtctgacctt cgcttactcg gtgttgttta ccaccacttt ggaaaaccgc gtacatataa
    28801 aactccgctt ccgcgcacac cttttcaatg attcagagtg cgctataaat attcacaata
    28861 gggcgcgaat attacgcaaa acgcacgcct ttgacaagtg ctacagtcaa tacacgaaga
    28921 aaaaaaacac aacttgtacg gtaacgttta tctgtgccat tttttcagta caatcactct
    28981 atattcctaa ccataaaccc taagttgcct ttgttcacag taaggtaatc ggggcgaaaa
    29041 gcccggcttt tgcgatgaat ttagaaaaaa tcaatgagtt aaccgcgcaa gatatggcgg
    29101 gtgttaatgc ggcaatcctt gagcagctta attccgacgt ccagctgatc aatcagttag
    29161 gctattacat cgtcagcggc ggcggtaaac gtattcgtcc gatgattgct gtactggctg
    29221 cacgagctgt tggctatgag ggaaatgcgc atgtcaccat tgctgccctg atcgagttta
    29281 tccacacggc gactctgctg cacgacgacg ttgtggatga atcagatatg cgcaggggta
    29341 aagctaccgc caacgctgca tttggcaatg ccgccagcgt gctggtaggc gattttattt
    29401 atacccgcgc gttccagatg atgaccagcc tcggttcgct caaagtgctg gaagtcatgt
    29461 cagaagccgt aaacgtcatc gcagaaggtg aagttctgca actgatgaac gttaacgatc
    29521 cggacatcac tgaagaaaac tacatgcgcg ttatctatag caaaaccgcg cgtctgtttg
    29581 aggctgccgc gcagtgttcc gggattctgg ctggctgtac gccggaagag gagaaaggcc
    29641 tgcaggatta tgggcgctat ctcggcactg ctttccagtt gatcgacgat ttactcgatt
    29701 acaatgccga tggcgaacag ttaggtaaaa atgtcggcga cgatctgaac gaaggtaaac
    29761 cgacgctgcc gctgctgcat gcgatgcatc atggcacacc agaacaggca cagatgatcc
    29821 gtaccgccat cgaacagggt aacggtcgcc atcttctgga accggttctg gaagcaatga
    29881 acgcttgtgg atctcttgaa tggacgcgtc agcgtgccga ggaagaagca gacaaagcca
    29941 tcgcagcgtt acaggtgctc ccggacaccc cttggcgaga agcactcatc ggcctcgcgc
    30001 acatcgctgt tcaacgcgat cgttaatccc ctcccctcat cccgcgcagc gcgcggggtg
    30061 gttccagtaa attccataaa aacacttact cagctctata acacctgaaa aacaggctgt
    30121 gcatctttat atattctgaa tattcacact ccttacagga actttttaga gcaatcggcc
    30181 atcaggagta tagtgatgct cgacagaaga agtgttctga atgaaagcga ctaacttaag
    30241 gagtgaggaa agatggaaag taatttcatt gactggcatc ccgctgacat cattgcgggt
    30301 ttgcgcaaga agggaacgtc aatggcggcg gagtctcgca gaaatggttt gagttcctca
    30361 acgctggcga atgcattatc gcgcccatgg ccgaaaggag agatgattat tgcgaaagcc
    30421 ctgggaactg acccctgggt tatctggcca tcacgctacc atgatccgca gacccatgag
    30481 tttatcgaca gaacgcagtt gatgcgtagc tacactaaac cgaaaaaatg agtggtctgg
    30541 cggtagcccc gcgaacgggg ctgccagctc tcagacgatt actcgccttt cacacgctca
    30601 atatttgcac ctaaagcgcg cagtttgtct tcaatgcgtt cgtagccacg atcgatgtga
    30661 taaatacgat caaccaccgt cgtcccttcc gcaatacagc cagccagcac caggcttgct
    30721 gatgcacgca gatcggttgc cataacctgt gcgccagaaa gtttttcaac accgtgacaa
    30781 ataacggtat tgctttcgat ttcggcatgc gcgcccatac ggctaagctc cggcacatgc
    30841 ataaagcggt tttcaaagac cgtttcggtg atgaatccgg tcccttctgc caccaggttc
    30901 aacagcgtga actgggcctg catatcggtc gggaatgccg gatgcggcgc ggtacgtacg
    30961 ttaacagcct tcggacgttt gccatgcata tccaggctaa tccagtcttc gccgacttcg
    31021 atgtccgctc cagcgtcacg cagtttcgcc agcacggcgt cgagagtatc tggctgcgcg
    31081 ttacggcaga taattttgcc gcgagaaatc gccgccgcca ccaggaaagt accggtttcg
    31141 atacgatccg gtagcacgcg atagacaccg ccgcctaaac gttccacacc ttcgatgacg
    31201 atacgatcgg tgccctgacc gctaattttc gcacccagcg taatcaggaa gttcgcggta
    31261 tcgacgattt ccggttcacg cgctgcgttt tcaataatcg tggtaccttc cgccagggtt
    31321 gcagcacaca tgatggtcac cgttgcgcca acgctgactt tatccatcac gatatgtgcg
    31381 cctttcagac gaccatcgac ggaagcttta acgtaacctt cttccagttt gatggtcgcg
    31441 cctaattgtt cgaggccaga aatgtgtaga tcaaccggac gcgcaccgat cgtacaaccg
    31501 ccaggcagtg aaacttgccc ctgaccaaag cgcgctacca gcggccccag tgcccagata
    31561 gaagcacgca tggttttaac cagatcgtaa ggtgcgcaga atacattaac gtcgcgggca
    31621 tcaatatgca cagaaccatt acgttctact ttcgcaccca gctggcttag cagcttcatt
    31681 gatgtatcga cgtctttcag tttcgggacg ttctggatct ctaccggttc ttccgccagt
    31741 agagcggcaa aaaggatagg cagagcagca tttttagcgc cggaaattgt gacttcgccc
    31801 tggagcttcg ttggcccctg aacacgaaat ttatccattt agtttgttct cagttaacaa
    31861 ttcatatccg ctaccggcga atcgcccata gctcaaaagc cgttcagttt gcgatcgcgc
    31921 gcccactccg caggggtata cgctttgatc gacacagcat gaatgcggtt atccgcaata
    31981 tattccatca gcgggccata gaccgtctgc tgttttttaa cccgactcat gccgtcaaac
    32041 aactcaccca cggcaataac ctgaaagtgg ctgccatcgc cggaaacgtg gacttcctgg
    32101 agggagagag cgttcatcaa cacgctctga atttcattat tttccatggg atcttcaatc
    32161 atcagttaat aaaccagcga aacatcttag agcaaagttg cgctggcata aataagcaaa
    32221 aagcctcgct gataaatcag acaaggctcg acttgcaggc aggtttgccg gacaggcggt
    32281 taacgccata tccggcctga aaaaatttaa cgaggcagaa catcagcagg caaattatac
    32341 aatttcgcga gggtatacac tttgtcgttt accccctgaa gcgtcacgtt gttgccctgc
    32401 tttttcgcca gatcgataag atggagcagc agtgccagtc cacccgtatc cacgcgggac
    32461 acacggctaa gatcgatgca ggtaatcccc ttcaccgctt cctcacgcat ttcccaaagc
    32521 ggtagcagaa cgtcctgatc cagctctccg gataacgcca gcgtgtcacc cgtctgcatc
    32581 cagctcagtg actcgctcat tattttttct cttccagagt gattttctgt tgagaaatcg
    32641 atttcagttg cgcagtcagg ccgtcgatac ctttggtacg cagcagcgtt ccccactcgt
    32701 tttgtttggt ggtgatcata ctgacgcctt cagcaatcat gtcgtaagcc tgccagtttc
    32761 ccgtctggga gtttttacgc cactggaagt ccagacgcac cggcggacgg ccattcgggt
    32821 caataatggt aacgcgaata ggcacaatgg ttttatcgcc cagcggctgt tctggcgcaa
    32881 tctgataggt ttgaccgtga tacatcgcca gcgcctgacc gtaagcctgc ttcaggtact
    32941 cacggaaagc ggcaaagtag gcttcacgtt gagcaggggt cgcactcttg taatactggc
    33001 ccagcaccag cgcaccggcg tatttcacct gtacgtatgg cagcagttcc tgatcaacaa
    33061 tggtacgcag ataatccggg ttggcccgaa tttgcggttg ctcattcttc aggcgatcga
    33121 acgttttctg cgccgcctcg tccatcagct tatacggatt ggtctggtct gccgcggttg
    33181 ccgcactcag aggtgcaatc accagcaaag cgaccatcat taaacgttta aacatgcgtc
    33241 ggttctcctg aaattatttc gttgtaccca caggttcagt ggtttcatta ttacctggcg
    33301 cagcagctgg cgcatcgcca ctattcttat tgtcatcgcc tttactaccg taaaggaact
    33361 gaccaatgag atcttccagc accatcgcag acttagtgtc ctgaattgta tcgccatcct
    33421 tcaggatagc agtccccagt tccgggtctt caaaaccgac gtttaatgcc agatattgtt
    33481 cccccagcag gccggaagta cgaatgctca gcgaactggt atctggaatg tggttataac
    33541 gttgttcaat ttccagcgtt acgcgtggca gataggtttt cgggtccagc gtaatatccg
    33601 ccacccggcc cacaacaacg ccaccaatac tgaccggaga gcgcgctttc aggccgccaa
    33661 tgttatcgaa cgtcgcataa agcgtgtagg tcggttcagt acgtatggac gtcacgttcg
    33721 ccgccttcag gcaaacaaac agcgccgcca gcagtgctgc taataaaaag atacccaccc
    33781 aaatttcatt ttttttcgtt tgcatgaact caattcccaa acatcaatgc ggtcagcaca
    33841 aaatccagcc ccagaacagc cagagacgag tggacaacgg tgcgagtggt tgcccggcta
    33901 atcccggcag acgtcgggat ggcgtcgtag ccgttaaaca acgaaatcca cgtcaccgtg
    33961 atggcgaaca ccacgctctt aatcagacag ttgaccagat ccatacgcca gtcgacggca
    34021 ttttgcattg ccgaccagaa gaacccgcta tcaatgcctt tccaactgac gccgaccaga
    34081 gatccgcccc agatccccac ggcgacgaaa ataaccgtca acagtggtaa tgaaataacc
    34141 ccagcccaga aacggggaga aataacccga cgcagcggat ccaccgccat catctccata
    34201 ctggagagtt gctctgtagc gcgcatcagg ccgatttctg cggttagcgc cgaaccagca
    34261 cgcccggcaa acaacaacgc ggcaaccacc ggccccagtt cacgcagtag cgataacgcc
    34321 accagcatac ccagactggt ttccgcacta taagtggtca gaaccagata accttgcagc
    34381 cccaacacca ttccgatgaa cacgccagaa accacaataa tcagcatcga caggacgccg
    34441 acattataga gctggcgcac cagcagcggc gcatgtttgc gaaattccgg tttgccgacc
    34501 agcgcattga ataacattaa cccggcccgc ccgaacgttc tcagggtttt aatcccttta
    34561 tgtccgagcg acgccagcgc atttaacagc atgagtggct taactccctg gtaaaagatc
    34621 agcgtgataa tcgccggcag gatagcggaa cggaacaggc ccgtcagcta tcccgtccag
    34681 aaactgacgt acgcgcggat caggattcgc ctgcaacgcc tgggcactgc catgagcgac
    34741 aatttttttg tccgccagga tccaggcgtg atccgcaata cttaacactt ccggcacatc
    34801 gtgagaaacc accacacaag tcacgcccag cgcgctgttc agctcagaaa tcagcttcac
    34861 cagtacgccc atggtgatgg gatcttgccc aacaaaaggt tcatcaaaca tgatgagatc
    34921 cggctccagc gcaatcgcac gcgccagcgc tgcacgccgc gccatcccac cagaaagttc
    34981 agaaggcatt agcttagccg ctccacgcag ccccacggcc tccagcttca tcatcactgt
    35041 actatgcaac aatggcgcgg gaagttgggt atgttcgcgc agtggatagg cgacgttgtc
    35101 aaatacgttc atatcagtga acaacgcccc ggactgaaat aacatgctca tccgtttgcg
    35161 cactgtatac aggcgcgaac gagacatcgc cggaatattc tcaccatcga aaaggatctc
    35221 accatgatct ggtgcgattt gcccgccaat cagacggagt agcgtcgttt taccgatgcc
    35281 cgatggcccc atgattgccg tgatcttccc tcgcggcacg gtcagggaaa tattatcgaa
    35341 gatgcagcga ttgccacgcg taaaactgac atcgcgcata tcgactaaat tcgccacaga
    35401 ctgctccata attcaccctt cgtcttgcgt tgattttcta agcatggcgc tcaatttaac
    35461 cttgaaccca acatatttac agaatattac ccgccgtggt tagcgaaagc tggcatttgt
    35521 tttacttttt agccgcataa agtcaaaatt aagaatccgt tacggctttc tgaaaatctt
    35581 cagcggaccg gcgagtatac ctgaagaaag gatgttagat gcttttagct acggcactgt
    35641 taattgttgg tttacttttg gtcgtttaca gtgccgaccg cctggttttt gccgcgtcta
    35701 ttctttgccg aacctttggc atcccgccgc tgatcatcgg catgacggtg gtcagtattg
    35761 gtacatcgtt accagaagtc atcgtctcgc ttgccgcgtc tctgcacgaa caacgcgatt
    35821 tagccgttgg tacagccctc ggctcaaaca ttatcaatat attgctgatc ctcggtctgg
    35881 ctgcgctggt tcgtcctttt accgtccatt ctgatgttct acgccgtgaa ttacccttaa
    35941 tgttgttggt cagcgtggtg gccggttccg tactctatga cggacaactt agtcgcagcg
    36001 atggtatctt tctcctcttt ctggctgtgc tatggctgct gttcattgtt aaacttgcac
    36061 gtcaggctga acgtcagggg actgacagcc tgaccagaga gcagcttgca gagctgccgc
    36121 gtgacggcgg attgcccgtc gcgtttttat ggctcggcat tgcgcttatc atcatgccag
    36181 tggccacgcg gatggtggtt gataacgcca cggtgctggc gaattacttt gccatcagcg
    36241 agttgacgat gggtctgacg gcaattgcta tcggaaccag cctgccggaa ctggcaaccg
    36301 caatagcggg ggttcgcaaa ggtgaaaacg acattgctgt cggaaatatc attggcgcaa
    36361 acatttttaa tattgtcatc gtgttgggtt tacccgcgct gataacgcca ggagagattg
    36421 atccactggc gtacagtcgt gactacagcg tgatgttgct ggtgagcatt atttttgcgt
    36481 tgctgtgctg gcggcgctcc ccacaaccgg gccgtggtgt aggggtatta ttaactggcg
    36541 gatttatcgt atggctggcg atgttgtact ggttatcgcc aatactcgtt gaataactgg
    36601 aaacgcatta tgtcgcacgt agagttacaa ccgggttttg actttcagca agcaggtaaa
    36661 gaagtcctgg cgattgaacg tgaatgcctg gcggagcttg atcaatacat caatcagaat
    36721 ttcacgcttg cctgtgaaaa gatgttctgg tgtaaaggga aagttgtcgt catggggatg
    36781 gggaaatcgg ggcacatcgg gcgcaaaatg gccgcaacgt ttgccagcac cggtacacct
    36841 tcatttttcg tccatcctgg tgaagccgcg catggtgatt taggcatggt caccccacag
    36901 gatgtggtga ttgctatctc taactcaggt gaatccagcg aaatcacggc cttaattcca
    36961 gtgcttaagc gtcttcacgt accgttaatc tgcatcaccg gtcgcccgga gagcagcatg
    37021 gcgcgcgctg cagatgtgca tctgtgtgtt aaagtagcga aagaagcctg tccgttaggg
    37081 ctggcaccga ccagcagcac caccgccacg ctggttatgg gcgatgccct cgctgtcgcg
    37141 ctgttaaaag cacgcggctt tactgctgaa gattttgcgc tctcacaccc aggcggcgca
    37201 ctgggtcgta aacttctgct gcgcgtaaac gatattatgc atacgggcga tgagatcccg
    37261 catgttaaga aaacggccag tctgcgtgac gcattgctgg aagttacccg caaaaatctt
    37321 ggtatgactg tcatttgcga tgacaatatg atgattgaag gcatctttac cgacggtgat
    37381 ttacgccgtg tcttcgatat gggcgtggat gttcgtcagt taagtattgc cgatgtgatg
    37441 acgccggggg gaatacgtgt gcgccctggc attctggccg ttgaggcact gaacttaatg
    37501 cagtcccgcc atatcacctc cgtgatggtt gccgatggcg accatttact cggtgtgtta
    37561 catatgcatg atttactgcg tgcaggcgta gtgtaaagat tcaaggataa acaacaatga
    37621 gcaaagcagg tgcgtcgctt gcgacctgtt acggccctgt cagcgccgac gttatggcaa
    37681 aagcagagaa cattcgtctg ctgatcctcg acgttgatgg cgtactgtca gatggcctga
    37741 tttatatggg caataacggc gaagagctga aagcgttcaa tgttcgtgac ggttatggca
    37801 ttcgttgtgt gctcacctct gatattgaag tcgctatcat taccgggcga aaggctaaac
    37861 tggtagaaga tcgttgtgcc acattgggga tcactcactt gtatcagggg cagtcaaaca
    37921 aactgatcgc ctttagcgat ttgctggaaa aactggcgat tgccccggaa aatgtggctt
    37981 atgtcggcga tgatctcatc gactggccgg taatggaaaa agtgggttta agcgtcgccg
    38041 tggccgatgc gcatccactg ttgatcccgc gcgccgatta cgtgacgcgc attgctggcg
    38101 gtcgtggcgc agtgcgcgaa gtttgcgact tattactcct ggcgcagggc aaactggatg
    38161 aagccaaagg gcaatcgata tgagtaaagc cagacgttgg gttatcattg tgctatcact
    38221 ggcggttctg gtgatgatcg gcattaatat ggccgaaaaa gacgataccg cccaggtggt
    38281 cgtcaacaac aatgatccca cctataaaag cgagcatacg gacacgctcg tctataaccc
    38341 agaaggggca ctaagctatc gattgattgc tcaacacgtt gaatattatt ccgatcaggc
    38401 cgtttcgtgg tttacgcagc cggtacttac cacgtttgat aaggataaaa tcccgacatg
    38461 gtccgtaaaa gcagataaag ccaagctgac caatgaccgg atgctctatt tatatggaca
    38521 cgttgaagtc aacgcactcg tgccggactc tcaacttcgc agaatcacga cggataacgc
    38581 gcagatcaat ctggtgacgc aggatgttac ctctgaagac ctcgtcacgt tatacggaac
    38641 aacatttaac tccagcggtc tgaaaatgcg cggcaactta cgcagcaaga acgccgagct
    38701 gattgaaaag gttagaacat cctatgaaat tcaaaacaaa caaactcagc cttaatcttg
    38761 tgcttgccag ctcacttctg gccgccagca ttccggcatt tgccgtaacc ggagacactg
    38821 atcagccgat ccacattgaa tcggaccagc aatctcttga tatgcaaggc aacgtggtta
    38881 cctttaccgg taatgtcatc gtcacccagg gcaccatcaa aattaatgcc gacaaagtgg
    38941 ttgttacccg tccgggcggc gaacaaggta aagaagtgat tgacggctac ggtaaaccgg
    39001 caacgttcta ccagatgcag gacaacggta aaccggttga aggtcacgct tcccagatgc
    39061 actacgaact ggcaaaagat tttgtcgttc tgacgggtaa tgcttatctg cagcaggttg
    39121 atagcaacat taagggcgat aagatcactt acctggtaaa agagcagaaa atgcaggctt
    39181 tcagcgacaa aggcaagcgc gtaaccaccg ttctggtgcc gtcgcagctg caggacaaaa
    39241 acaacaaagg ccagaccccg gcacagaaga agggtaatta attcgttatg gcaacattaa
    39301 ctgcaaagaa ccttgcaaaa gcctataaag gccgtcgcgt ggtagaagac gtcagcctga
    39361 ccgtcaactc cggggaaatt gtcggtctgc tggggccaaa cggtgccggt aagaccacca
    39421 ctttctatat ggttgtaggc attgtgccgc gcgatgcggg caacatcatt attgatgatg
    39481 acgatatcag tctgctgcct ctgcatgcac gcgcgcgccg cggtatcggc tatctgccac
    39541 aggaagcctc cattttccgt cgcctcagcg tttacgataa cctgatggcg gtactgcaaa
    39601 ttcgtgacga cttgtctgct gaacaacgtg aagaccgcgc gaacgagctg atggaagagt
    39661 ttcacattga gcacctgcgt gacagcatgg ggcagtcact ctccgggggt gaacgtcgcc
    39721 gtgtagaaat tgcccgcgca ctggctgcga atccgaaatt tattctgctc gacgaaccgt
    39781 ttgccggtgt tgacccgatc tcggttatcg acattaaacg catcattgag cacctgcgcg
    39841 acagcggcct gggcgtgctg atcactgacc acaacgtgcg tgaaacactg gcggtttgtg
    39901 aacgcgctta tatcgtcagt caggggcatt tgatcgccca cggcacgcct acagaaatct
    39961 tacaagacga acacgttaag cgtgtatacc ttggggaaga cttcagactc tgatagggta
    40021 gaagtttgcg acgttttagc aggagagtac gattctgaac atgaagcaag gtttgcaact
    40081 caggcttagc caacaactgg cgatgacgcc acagctccaa caggcaattc gtctgttgca
    40141 gttgtcgacg ctggaacttc agcaggagct acagcaggcg ctggagagta atccgctgct
    40201 tgagcaaatc gacactcatg aagaaatcga cacccgcgaa acgcaagaca gtgaaacgct
    40261 ggacaccgcc gatgcgctcg aacaaaaaga gatgccggaa gagctgccgc tcgatgccag
    40321 ttgggacacc atttacaccg ctggtacacc atccggcacc agcggtgact acattgacga
    40381 tgagctgccg gtctatcagg gcgaaacgac gcagaccttg caggattacc tgatgtggca
    40441 ggtcgagctg acaccgtttt ccgacactga ccgcgctatt gctacctcta tcgtcgatgc
    40501 cgttgatgac accggttatc tgactgtccc gctggaagat attctcgaaa gtatgggcga
    40561 tgaagagatc gacatcgacg aggttgaagc cgtccttaag cggatccaac ggtttgatcc
    40621 ggtcggtgta gcggcaaaag atctgcgtga ctgcctgcta atccaactct cccaattcga
    40681 taaaaccacg ccgtggctgg aagaggccag attgatcatt agcgatcatc tcgatctgtt
    40741 agccaatcac gacttccgca ctttaatgcg cgtcacgcgt ctgaaagaag atgtgctgaa
    40801 agaagccgtc aatctgatcc agtcgctcga tccgcgcccc gggcagtcga tccagactgg
    40861 cgaacctgag tatgtcattc cagatgtgct ggtgcgtaag cataacggtc actggacagt
    40921 agaactcaac agtgacagca ttccgcgtct gcaaatcaac cagcactacg cctcgatgtg
    40981 caataacgct cgcaatgatg gtgacagcca gtttatccgc agcaatctgc aggatgccaa
    41041 atggttgatc aagagtctgg aaagccgtaa cgatacgcta ctgcgcgtga gtcgctgtat
    41101 cgttgaacag cagcaagcct tctttgagca aggcgaagaa tatatgaaac cgatggtact
    41161 ggccgatatc gcccaggctg tcgaaatgca tgaatcgacg atatctcgcg tgaccacgca
    41221 aaaatacctg catagtccac gaggcatttt tgaactgaag tatttctttt ccagtcacgt
    41281 caataccgag ggcggcggcg aagcttcctc cacggcgatt cgtgcgctgg tgaagaaatt
    41341 aatcgcggcg gaaaacccag cgaaaccgtt gagcgacagc aagttaacct ctttgctgtc
    41401 ggaacaaggt atcatggtgg cacgccgcac tgttgcgaag taccgagagt ctttatccat
    41461 tccgccgtca aaccagcgta aacagctcgt ttgacccaac cgataaggaa gacactatgc
    41521 agctcaacat taccggaaat aacgtcgaga tcaccgaggc actgcgcgaa tttgttacag
    41581 ccaaatttgc caaacttgag caatattttg accgaatcaa ccaggtctat gttgttctga
    41641 aagtggagaa agtcacccac acctcagatg caacactgca tgtaaacggt ggtgaaattc
    41701 atgccagcgc ggaaggtcag gacatgtacg ctgccattga tggcttaatt gataagctgg
    41761 cacgccagtt gaccaaacat aaagataaac tgaaacaaca ctaattgtcc gggcaattag
    41821 catgtgcatg gcggcctgtt gtgcggcaca acgggccatt tgtacggtta atgctccgag
    41881 cctgttccac tgtttgagtg ggcaggttct taggtgaaat tatgacaaat aatgatacaa
    41941 ctctacagct tagcagtgtt cttaacaggg aatgtacgcg aagccgcgtc cactgtcaga
    42001 gcaaaaaacg cgccctggaa atcatcagcg agctggcggc gaaacaactt agcctgccgc
    42061 ctcaggtggt ttttgaagct atcctgacgc gggaaaaaat gggcagtacc ggtatcggca
    42121 atggtattgc cattccgcat ggcaaactgg aagaagatac tctgcgcgcc gttggcgttt
    42181 tcgttcagct cgaaacgcct atagctttcg atgccatcga caaccaaccg gtggacttac
    42241 tttttgccct gctggtgccg gcagaccaaa ctaaaacgca cctgcatact ctgtcgctgg
    42301 tggcgaaacg tctggcggac aaaaccatct gccgccgttt acgcgcagcc cagagcgatg
    42361 aagagctgta tcaaatcatt acggataccg aaggtactcc ggatgaagcg tagttattcg
    42421 gtaatgtctc ttttagacgt tgtgaggaga aacagtacat ggtactgatg atcgtcagcg
    42481 gacgttcagg ttcaggtaaa tctgtcgccc tgcgtgcgct ggaagatatg ggtttttact
    42541 gcgtggataa ccttcccgta gtgttgttac ccgatctggc tcgaactctg gccgatcgcg
    42601 agatttctgc cgccgtcagc attgatgttc gtaatatgcc ggagtcacca gaaatattcg
    42661 aacaggcgat gagtaacctg cctgacgctt tctcaccgca actactgttc ctggatgccg
    42721 accgtaatac cttaattcgt cgttacagtg acacgcgccg actgcatccg ctttccagca
    42781 aaaacctgtc gctggaaagt gctatcgaca aagaaagcga tttgctggag cctctgcgtt
    42841 cgcgagcgga tctgattgtc gatacctcag aaatgtccgt tcacgagctg gctgaaatgc
    42901 tgcgtacccg tctgctgggt aaacgtgaac gcgaactgac catggtcttt gagtctttcg
    42961 gcttcaaaca cggtatccct atcgatgcag attacgtctt tgacgtgcgc ttcttgccga
    43021 acccgcactg ggatccgaaa ctgcgtccaa tgacaggtct tgataaacct gtcgccgcgt
    43081 tcctcgaccg ccacacagaa gtacacaatt ttatctacca gacgcgaagc tatcttgaac
    43141 tatggttacc tatgctggaa accaacaacc gtagctactt gacggtcgcc attggttgta
    43201 ccggcggaaa gcaccgttcg gtgtatattg cagagcaact ggcagactac ttccgctcgc
    43261 gcggtaaaaa cgtccagtca cgccatcgta cgctggaaaa acgtaaacca tgaccgtcaa
    43321 gcaaactgtt gaaatcacaa acaagctggg catgcatgcc cggcctgcaa tgaagctgtt
    43381 tgaattaatg cagggttttg acgctgaagt gctgttacgt aatgacgaag gcaccgaggc
    43441 cgaagccaac agcgttattg cgctgctgat gttggattct gccaaaggac ggcagattga
    43501 agttgaagcg accggcccac aggaagagga agcactggcc gccgttatcg ccctctttaa
    43561 ctctggtttt gatgaagatt aatcttcatc actttgacat acaactccct tcaaactccc
    43621 cccgacaata agaaaatcac gtactgaaat cgttctcaat caacgtcatt tgtacatttt
    43681 gtgcgctttt cactttcaga agaaccttaa gaaaacctta agagacattg tttaggtttt
    43741 gtttaagtta atcgaccata ctggagatcg tcagaaaata tttccaggag atggcatgat
    43801 tcgcttatct gaacaaagtc ccctgggcac cggaagacat cggaaatgtt atgcgcatcc
    43861 ggaagatgcc caacgctgta tcaagattgt ctaccatcgt ggcgatggtg gcgataaaga
    43921 gatccgccgt gagttaaagt actacgcgca tcttggtcgc cggttaaaag actggagtgg
    43981 aataccgcgc tatcacggta ccgttgagac ggattgcgga accggttatg tctacgatgt
    44041 gattgcagat tttgacggca aaccctccat tacgctgacc gaatttgctg aacaatgccg
    44101 ttacgaagaa gacattgcac aactgcgcca gctactgaaa cagctgaagc gttatttgca
    44161 ggataaccgt atcgtgacga tgtcgctgaa gccgcagaat atcctctgtc accgcatcag
    44221 tgagtcagag gttatcccgg tcgtctgtga caacattggt gaaagcacat tgattccact
    44281 ggcgacctgg tcgaagtggt gttgtctgcg taagcaagag cggttatgga aacgatttat
    44341 cgcccaaccg gcgctggcta ttgccctgca gaaagatctg caacctcgtg agagcaaaac
    44401 gctggccctc acttcgcgcg aagcttaatc cagctggtgt tgttgcataa acggctcacc
    44461 gcctaactga tacatctgcc gtaaaatcca cgcctgacgg ctacgcacgt agcccgatgg
    44521 cgaggagact ttgaaacgaa gtggattagg taatacagct gccagtaatg cagcttccga
    44581 ccgggtaagt ttgctcgcgg gtttgtggaa ataacgttgt gccgcagctt cgacgccaaa
    44641 cacaccgtcg ccaaattcgg cgatattcag gtaaaccgtc aggatacgct ttttgctcca
    44701 gactgtttct atccccagcg ttaatccagc ttccagccct tttcgtaccc agctacgccc
    44761 atcccataaa aagagatttt tggccgtctg ctgagaaatc gttgaagcac cgcgaatacg
    44821 gttttcattg cgctcgttgt gcgccagggc tttctcaatg gaagcgacat caaagcccca
    44881 gtgctcagga aatttctgat cttctgcggc aatcaccgcc agtcccatcc acggcgagat
    44941 ttgatccata ctgacccagt cagaatgtgc cacgtaacga aaattgccat gcagccaggc
    45001 gctgacctgt cgctcgacca ttaccgctga gaagggaaca ggcgcaacac taaacaacgc
    45061 gatgcccccg ccccagaaaa cggcgaggac aaccattaac cgcaaaagaa aacggcgaac
    45121 gaaactaaac accgttaagc ggcttttact cattcagcca gaaccagcac gcgggaaacc
    45181 agcttatcaa tgccgctcgc cgcttctgca atgttctgcg ccagcatata tgctggggtg
    45241 gtgacaatct tattgtcttc atcaaccacg atatcatcga caggacaggg cacatgctcc
    45301 gcgcccatct cttccagcac ttctgcggta tcgatatcag taccgatggt caaacgcagc
    45361 gggaaatcga aaattttcgg cagcatcgcc ggggcaatac acataaaacc aagcggtttt
    45421 ccggcctgat gcatcgcttg tgccagcgcc tttaattcac ggtcaacggt gcattcgcta
    45481 ccaagagtgg caaaattgct taaattcttc gccgcgccaa acccccccgg cacaatcaac
    45541 gcatccagtt cagcggcatc ggcctgggcc agaggacgga tttcaccgcg cgttattcgt
    45601 gccgcttcaa tcagcacgtt gcgcgtttcc gtcatcgctt cgccagttaa atggttgata
    45661 acatcaacct gctgcttatc cggtgcaaag cagaccgcct gcgcaccgct gcgtgaaata
    45721 gctaacaacg tcaacaccgc ttcatgaatt tcagaaccgt catagacgcc gcatccgctc
    45781 agaattacgc caattttctt cattgtgatc atcctttcgc aattgactga aacacattaa
    45841 tttttttaat aaaaatggta cgcatcacac atttaactga ttcatgtaac aaatcattta
    45901 agttttgcta tcttaactgc gtgcggcctg aaaaacagtg ctgtgccctt gtaactcatc
    45961 ataataattt acggcgcagc caagatttcc ctggtgttgg cgcagtattc gcgcaccccg
    46021 gtctagccgg ggtcattttt tagtggcttt tgccacccac gctttcagca cttctacgtc
    46081 gtgacgccac tcttctttca tctcttcaat ccattcaccg acgttatctt cccaggcagg
    46141 aaggtccgga gactgaattt gctgacccag ctgttgcaga tggcgtaacc ccactgaccc
    46201 cgccgcacct ttaattttat gtccttcctc tacaatgcct tttttatcct gcgccgtcag
    46261 attcgactcc agcacgctga catagcccgg catcatcttc tcaaacaccg ctaacccgtc
    46321 ggtgatcagc ttcggtccta caagttcgag atactgttcc agcatgggaa tatcgagcaa
    46381 tgcttctgat ttactgttct cttctgtcgt caccgtactc tcctcatcat cctgggtatc
    46441 ccagaatttc ttgatcatcg cggttagcgc cggaaccgaa agcggcttac tcagcacatc
    46501 atccattcca gcattgaggt actcttgttt gtctttcagc acgttagcgg ttaaggccac
    46561 cagcggcggt aaatcctcgc gcggataacg cttcgtcagt tcacgagaga tatccagccc
    46621 ggtcatatct ggcaactgaa tatccagcaa caccaggtcg tattcgcccg gtttaaacat
    46681 ctccagcgcc gccttgccgg tcatggcgac atcaacgctg ttacctaatt tttccagcac
    46741 agaacgcgca acaatcacgt tcagttcaat gtcttccacc agcagcacat tcagcgccgg
    46801 taaaggcata tcgtcttcat caaacgcatc atcgacctct tctgctaccg acggtgcgtg
    46861 gatcgtcaac gtaaaggttg aacctttgcc ctgttcgctg gtaaccgtaa tatcgccgcc
    46921 catatttttc gccagacgac gagaaacggc cagaccaata ccggtgccgg tggcaggttt
    46981 accgccatga ctgtctttca cctggtaata catggcgaaa attttatcca gctcatcctg
    47041 cggaatgccg ataccagagt cttccacttc aaaatgcagc atatcgcctt catcgtagcg
    47101 cacgcgcacg gtaacctggc cttgctgggt gaatttgacg gcgttactga tgaggttcca
    47161 caggatctgc cgtaaacgcg tcccgtcggt aatgacctga tgcggtaatg gcagcgtcgg
    47221 ctccaggtta aagcgcaatc ctttttgttg cgcctgcaat gcggagagat tttccagatc
    47281 ggcaaggaag ctggtgaaat caaccggttg attatcaagc tggaccttgc gccgttccat
    47341 cttatccatg tcgataatat cgttaaagat attccccagc gtgacggccg aaacatggat
    47401 ggtcttgaga tatttttcct gctcggcggt gagttcggta tccagcagaa tacggctcag
    47461 gccgacgata ccgttcagcg gtgtacgcaa ttcgtgactg atggtggaga taaacgtcgt
    47521 tttgtcgcgg ctggcccgtt caagcgcatc ctgataccgc ttacgctcgg taatgtcgcg
    47581 accaaagccc atcaaaccgt gacgtttacc cacgcggtcg tagtacggca ctttacggat
    47641 ttcaaagcag gctttgcgcc cgtccgggta atccagccac tgttcatagg tcagtgacac
    47701 attatgacgg aacacttttt catcggtttc aatgactttt gcggcggctt ccggtgagta
    47761 aacatcagca ggtttcaggt gaaccagttg tttttcgctt tttccggtca gcagctccat
    47821 cgcgcggtta cagccggaaa actctttatc ttcgttacga taaaaaacca ggtcgggtga
    47881 agcatcaagg aaggaacgta agaatgagga ttgctgctcg agctgaattt gtgtctcttc
    47941 gcgctctttg atttcaattt tcagttggcc gaaggtttcc tgcagttctg cttccgcttt
    48001 ttcacgaacg gcgatttcct gatttagctg ggcaatatta tcttttaact gaacgttgag
    48061 gctcaaatcg cgctcgcgca tctcctccag tttttgcacc agccgtgaca gacgttgtcg
    48121 tgactcctcc agttgctcga cgaccaccga tagaaagtag accgcccacg gcgtaatcag
    48181 caaaccaaag aagatagaac gaataacatc aatgctttcg acctgaccat gcagcaccat
    48241 ggttaccgcc atttgtacca caatggcaag aacgacgagg gccagcgcca gcaacattga
    48301 gaagcgcacc agacctaact tcatcatcag gtcaacataa tactgcgcca gcagacgaat
    48361 ttgcttcatt agggaattcc ttcacgacaa cctgccccaa taatacccaa ttctgacagt
    48421 tacgttataa attgtgtgag aaatgcggaa ttcccctcaa cgacctactc cgttggaggt
    48481 atccacggac gttccagcgc cgatccctgt acgccatgtt cattcagata gcgatccagc
    48541 tcgaccattc ccgtccatcg attttcgcac cacagaggag caagcagcgt cgggcgacgg
    48601 gcgctggcgg aaatgcggtg gtagatcact tccggtggcg tatggcgaat catttctccg
    48661 gcagtgagcg tgtaatcctc cagttcaata ccgttcaaac gccccgcttc ccaggctttc
    48721 gccataatgc tgcctttcac aatatgcagc ggatgcagct ttatgccgtc cacgccggtt
    48781 tcaaccactc gctccagcgt ttgcaaacat tcagcctgcc cttcgccagg caggccgaca
    48841 attaagtggg aacacacctt cagcccgcgc tgacgtgcca gctgggttgt acgctgataa
    48901 caggcaaaat catggccgcg gttgatgcga tgcagtgttt tgtcgtgggc ggtttgtagc
    48961 cccagctcca gccacacttc gtaaccctgg tccttatatt cgcaaagcag atccagcacc
    49021 gcatccggca cgcagtccgg gcgggtacca acacacaaac cgacaatatt ggcctggctc
    49081 accgcctgct gatacataga acgcagcacc tgaacttccg caaaggtgct ggtatacgcc
    49141 tgaaagtagg ccagatagcg tttagcgcgg ttaactaaat tcgcctggtg cgccagttgc
    49201 tcggcaatgg aacgatgctg ctgcgcttca tcggcaaacg aggcaacatt acagaatgtg
    49261 cagccaccac gcccgatggt accgtcacgg ttagggcagc taaaaccgcc gtgcagcgtc
    49321 agcttatgca ccttttgccc ataacgacgg gtgagatcac caccaaacat attgactaat
    49381 ttctgtaact gcataatctg atagaccgcg ccttgaaaag aggccaaagc ctgccatttt
    49441 tagctcaatt cggcgatgac ctggatcaat cgtcccggcc tgcttttatc aactgcataa
    49501 tcaatcaaaa ttaccgaaat ttcatgcata atcacataaa tcacttttgc ttatcttgtg
    49561 tcagattttt ttatctcctt atggatttta ggcaaaaaca gtagcatgaa acgtcattac
    49621 caattaaggc agtataaaat gctggctttc tcgtcagttc aaggcaggat aagggttaac
    49681 acgcctttat gacagtcagg aattgactgt ttctctaacg acttccctt
//
LOCUS       DAZGLJ010000008        48943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000008 DAZGLJ010000000
VERSION     DAZGLJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.008"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            807..2024
                     /gene="alaA"
                     /locus_tag="ACHK49_000436"
     CDS             807..2024
                     /gene="alaA"
                     /locus_tag="ACHK49_000436"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO9261132.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            2108..2707
                     /gene="yfbR"
                     /locus_tag="ACHK49_000437"
     CDS             2108..2707
                     /gene="yfbR"
                     /locus_tag="ACHK49_000437"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO9261133.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2766..4598)
                     /locus_tag="ACHK49_000438"
     CDS             complement(2766..4598)
                     /locus_tag="ACHK49_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO9261134.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4685..5335)
                     /gene="hxpA"
                     /locus_tag="ACHK49_000439"
     CDS             complement(4685..5335)
                     /gene="hxpA"
                     /locus_tag="ACHK49_000439"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO9261135.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(5346..5840)
                     /locus_tag="ACHK49_000440"
     CDS             complement(5346..5840)
                     /locus_tag="ACHK49_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO9261136.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5923..6378)
                     /gene="yfbV"
                     /locus_tag="ACHK49_000441"
     CDS             complement(5923..6378)
                     /gene="yfbV"
                     /locus_tag="ACHK49_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO9261137.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6716..7918
                     /gene="ackA"
                     /locus_tag="ACHK49_000442"
     CDS             6716..7918
                     /gene="ackA"
                     /locus_tag="ACHK49_000442"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO9261138.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7993..10137
                     /gene="pta"
                     /locus_tag="ACHK49_000443"
     CDS             7993..10137
                     /gene="pta"
                     /locus_tag="ACHK49_000443"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO9261139.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            10327..11847
                     /gene="yfcC"
                     /locus_tag="ACHK49_000444"
     CDS             10327..11847
                     /gene="yfcC"
                     /locus_tag="ACHK49_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO9261140.1"
                     /translation="MSATTESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11880..12422)
                     /gene="yfcD"
                     /locus_tag="ACHK49_000445"
     CDS             complement(11880..12422)
                     /gene="yfcD"
                     /locus_tag="ACHK49_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO9261141.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12480..13031)
                     /gene="yfcE"
                     /locus_tag="ACHK49_000446"
     CDS             complement(12480..13031)
                     /gene="yfcE"
                     /locus_tag="ACHK49_000446"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO9261142.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(13087..13731)
                     /gene="yfcF"
                     /locus_tag="ACHK49_000447"
     CDS             complement(13087..13731)
                     /gene="yfcF"
                     /locus_tag="ACHK49_000447"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO9261143.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13867..14514
                     /gene="yfcG"
                     /locus_tag="ACHK49_000448"
     CDS             13867..14514
                     /gene="yfcG"
                     /locus_tag="ACHK49_000448"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO9261144.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14571..14933
                     /gene="folX"
                     /locus_tag="ACHK49_000449"
     CDS             14571..14933
                     /gene="folX"
                     /locus_tag="ACHK49_000449"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO9261145.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14954..15847
                     /locus_tag="ACHK49_000450"
     CDS             14954..15847
                     /locus_tag="ACHK49_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO9261146.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15895..16785)
                     /gene="rpnB"
                     /locus_tag="ACHK49_000451"
     CDS             complement(15895..16785)
                     /gene="rpnB"
                     /locus_tag="ACHK49_000451"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO9261147.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16982..17755)
                     /gene="hisP"
                     /locus_tag="ACHK49_000452"
     CDS             complement(16982..17755)
                     /gene="hisP"
                     /locus_tag="ACHK49_000452"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO9261148.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17763..18479)
                     /locus_tag="ACHK49_000453"
     CDS             complement(17763..18479)
                     /locus_tag="ACHK49_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9261149.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18476..19162)
                     /gene="hisQ"
                     /locus_tag="ACHK49_000454"
     CDS             complement(18476..19162)
                     /gene="hisQ"
                     /locus_tag="ACHK49_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO9261150.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(19252..20034)
                     /gene="hisJ"
                     /locus_tag="ACHK49_000455"
     CDS             complement(19252..20034)
                     /gene="hisJ"
                     /locus_tag="ACHK49_000455"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO9261151.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(20255..21037)
                     /gene="argT"
                     /locus_tag="ACHK49_000456"
     CDS             complement(20255..21037)
                     /gene="argT"
                     /locus_tag="ACHK49_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO9261152.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(21303..21872)
                     /gene="ubiX"
                     /locus_tag="ACHK49_000457"
     CDS             complement(21303..21872)
                     /gene="ubiX"
                     /locus_tag="ACHK49_000457"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO9261153.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21967..23484)
                     /gene="purF"
                     /locus_tag="ACHK49_000458"
     CDS             complement(21967..23484)
                     /gene="purF"
                     /locus_tag="ACHK49_000458"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO9261154.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23521..24009)
                     /gene="cvpA"
                     /locus_tag="ACHK49_000459"
     CDS             complement(23521..24009)
                     /gene="cvpA"
                     /locus_tag="ACHK49_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO9261155.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(24268..24930)
                     /gene="dedD"
                     /locus_tag="ACHK49_000460"
     CDS             complement(24268..24930)
                     /gene="dedD"
                     /locus_tag="ACHK49_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO9261156.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKSKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24920..26188)
                     /gene="folC"
                     /locus_tag="ACHK49_000461"
     CDS             complement(24920..26188)
                     /gene="folC"
                     /locus_tag="ACHK49_000461"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO9261157.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(26258..27172)
                     /gene="accD"
                     /locus_tag="ACHK49_000462"
     CDS             complement(26258..27172)
                     /gene="accD"
                     /locus_tag="ACHK49_000462"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO9261158.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(27328..27987)
                     /locus_tag="ACHK49_000463"
     CDS             complement(27328..27987)
                     /locus_tag="ACHK49_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO9261159.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(28070..28882)
                     /gene="truA"
                     /locus_tag="ACHK49_000464"
     CDS             complement(28070..28882)
                     /gene="truA"
                     /locus_tag="ACHK49_000464"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO9261160.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVMRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTRAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28882..29895)
                     /locus_tag="ACHK49_000465"
     CDS             complement(28882..29895)
                     /locus_tag="ACHK49_000465"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO9261161.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29961..31097)
                     /gene="pdxB"
                     /locus_tag="ACHK49_000466"
     CDS             complement(29961..31097)
                     /gene="pdxB"
                     /locus_tag="ACHK49_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO9261162.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            31196..32191
                     /gene="flk"
                     /locus_tag="ACHK49_000467"
     CDS             31196..32191
                     /gene="flk"
                     /locus_tag="ACHK49_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO9261163.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(32188..33366)
                     /locus_tag="ACHK49_000468"
     CDS             complement(32188..33366)
                     /locus_tag="ACHK49_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9261164.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33650..34870)
                     /gene="fabB"
                     /locus_tag="ACHK49_000469"
     CDS             complement(33650..34870)
                     /gene="fabB"
                     /locus_tag="ACHK49_000469"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO9261165.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            35029..37035
                     /gene="mnmC"
                     /locus_tag="ACHK49_000470"
     CDS             35029..37035
                     /gene="mnmC"
                     /locus_tag="ACHK49_000470"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO9261166.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFNQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(37156..37434)
                     /locus_tag="ACHK49_000471"
     CDS             complement(37156..37434)
                     /locus_tag="ACHK49_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO9261167.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37468..38016)
                     /gene="epmC"
                     /locus_tag="ACHK49_000472"
     CDS             complement(37468..38016)
                     /gene="epmC"
                     /locus_tag="ACHK49_000472"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO9261168.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(38016..38825)
                     /locus_tag="ACHK49_000473"
     CDS             complement(38016..38825)
                     /locus_tag="ACHK49_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO9261169.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLFDSHGQEILHWLGMN"
     gene            complement(38825..39649)
                     /gene="mepA"
                     /locus_tag="ACHK49_000474"
     CDS             complement(38825..39649)
                     /gene="mepA"
                     /locus_tag="ACHK49_000474"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO9261170.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSSLWKPEI
                     FSLIKLAAQDKNVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39653..40738)
                     /gene="aroC"
                     /locus_tag="ACHK49_000475"
     CDS             complement(39653..40738)
                     /gene="aroC"
                     /locus_tag="ACHK49_000475"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO9261171.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40773..41705)
                     /gene="prmB"
                     /locus_tag="ACHK49_000476"
     CDS             complement(40773..41705)
                     /gene="prmB"
                     /locus_tag="ACHK49_000476"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO9261172.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41871..42422
                     /gene="smrB"
                     /locus_tag="ACHK49_000477"
     CDS             41871..42422
                     /gene="smrB"
                     /locus_tag="ACHK49_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO9261173.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42495..43346)
                     /locus_tag="ACHK49_000478"
                     /pseudo
     CDS             complement(42495..43346)
                     /locus_tag="ACHK49_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721620.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
     gene            complement(43348..43887)
                     /locus_tag="ACHK49_000479"
     CDS             complement(43348..43887)
                     /locus_tag="ACHK49_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9261174.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGARAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVAGQSAHLPVVFKLKDCKGPAGYNVKVTLTGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
     gene            complement(43884..44372)
                     /locus_tag="ACHK49_000480"
     CDS             complement(43884..44372)
                     /locus_tag="ACHK49_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9261175.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTNFSAYATLRVD
                     YQ"
     gene            complement(44369..44878)
                     /locus_tag="ACHK49_000481"
     CDS             complement(44369..44878)
                     /locus_tag="ACHK49_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9261176.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(44894..45646)
                     /locus_tag="ACHK49_000482"
     CDS             complement(44894..45646)
                     /locus_tag="ACHK49_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO9261177.1"
                     /translation="MFNLTNTAKIVVPALALLATVVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFICTGNSCQVDEEQSRKG"
     gene            complement(45666..48311)
                     /gene="yfcU"
                     /locus_tag="ACHK49_000483"
     CDS             complement(45666..48311)
                     /gene="yfcU"
                     /locus_tag="ACHK49_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFO9261178.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHIEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDVGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGY
                     RYEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPA
                     VKPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(48393..>48943)
                     /locus_tag="ACHK49_000484"
     CDS             complement(48393..>48943)
                     /locus_tag="ACHK49_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9261179.1"
                     /translation="KTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCSIVPDD
                     HKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSANSGNYY
                     TIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNFKAWLV
                     GAADAPDLGNFEANTTFQITYL"
BASE COUNT        11980 a        13237 c        12203 g        11523 t
ORIGIN      
        1 agtaaaaatg catatgagtt gcactaaaaa agcgactcac attgttccgt tataatgcct
       61 gaagtagatc acagaatata tcttcaggga tcgcatatct attaagttac tcactctttt
      121 ctatttatga catgcgcgtg tttgtataaa tgtaaatgtg agtccttgtt ccactctcgt
      181 gcagcatcgc tggtcatacg cgaacacgta caaacagcaa tggtgaggcg gcatcaagag
      241 cagggatccg tcatttatgc gagcatttta ccccaaaaac cttttattta taagggtcat
      301 tgcgaattat ctgatgcaaa gttatgttat gttaggcaaa gtaatcttct attattaata
      361 agcacatcaa aacctttttg aatattaaat aataattaat tagcatcatc ctcattcatt
      421 aattccgctt aacaatagtt tcacaccttg cccccattac gacacctcgg aaatcatcac
      481 gctgttaaac tctccgtaat gcggaaatat catcatcgcg tttcattagt gaattcttaa
      541 atgaggcatt ttcacacaat tatcttacag ataaaaaaac cagacttaca attaagaatc
      601 agaacaattc actatataac attgcatgta aagcatatac acctcattat tttgtcatta
      661 ttaagttatt aacagcacaa tcgagccttc ccctctggca aaatcttatt ctgcagacct
      721 tcaaaacacc gtcctggggg agtacattgt tctaagctga cttccacggc agggagtggc
      781 gataacagca aaaaaggtca agattcatgt cccccattga aaaatccagc aaattagaga
      841 atgtctgtta tgacatccgt ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag
      901 gtaacaaggt actgaaactg aacatcggca acccagcccc gttcggtttt gacgcgccag
      961 atgaaatcct cgttgacgtg atacgcaacc tgcctaccgc tcaagggtat tgcgattcca
     1021 aaggtcttta ctccgcgcgt aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg
     1081 atgttaccgt ggaagatatt tacatcggca atggtgtatc ggagcttatc gttcaggcaa
     1141 tgcaggcatt gctgaacagc ggggacgaaa tgttggttcc tgcaccagat tacccactat
     1201 ggaccgcggc ggtttcgctt tccagcggta aagcggtgca ttatctttgc gatgaatcct
     1261 ctgactggtt cccggacctc gatgatattc gcgctaaaat tacgcctcgt acgcgtggga
     1321 tcgttattat caacccaaat aacccaaccg gcgcggtata ttccaaagag cttttaatgg
     1381 agattgtgga gattgcacgt cagcataatc tcattatctt cgccgatgaa atttatgaca
     1441 aaattcttta cgacgacgct gagcatcact caattgcgcc gctggcacct gacctgctga
     1501 ccattacctt taacggactg tcgaaaacgt accgcgttgc aggcttccgt caggggtgga
     1561 tggtgttgaa cgggccgaaa aaacacgcca aaggctacat cgaaggtctg gaaatgctgg
     1621 cttcaatgcg cctgtgtgct aacgttcctg cgcaacacgc cattcagacc gcgctgggtg
     1681 gttatcagag catcagtgaa tttattaccc ctggcggtcg tctttatgag cagcgtaacc
     1741 gcgcgtggga actgatcaac gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc
     1801 tgtatatgtt cccgaaaatc gacgccaaac gctttaacat tcacgacgat cagaaaatgg
     1861 tgctggattt cctgttgcag gaaaaagttc tgttggtgca agggacggca ttcaactggc
     1921 cgtggccgga tcacttccgc attgtcacgc taccgcgtgt cgatgatatc gagctgtctt
     1981 tgagcaagtt cgcgcgtttc ctttctggtt atcatcagct gtaatcttaa tttcactgcc
     2041 ggagattgca tccggcagcg ttatcccgcc acaatgacct gatgatgtca tcatacgtaa
     2101 ggtcactatg aaacagagcc atttttttgc ccatctctcc cgcctgaaac tcattaaccg
     2161 ctggccgctc atgcgcaacg tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc
     2221 gatggtcgcc catgcgctgg cagctatcaa aaatcggaaa tttggcggta atgtcaacgc
     2281 cgaacgtatc gctttactgg cgatgtacca cgatgcctca gaagtgctca ccggcgatct
     2341 ccctactccg gtgaaatact tcaattcgca aatcgctcag gagtacaagg ctattgaaaa
     2401 aatcgctcag caaaaactgg tcgatatggt tccggaagag ctgcgggata tctttgcgcc
     2461 gttaattgac gagcatgcat atagcgatga agaaaaatcg ctggtgaaac aggcagatgc
     2521 actgtgtgca tatctgaaat gtctggaaga actcgcggcc ggaaataatg aattcttgct
     2581 ggcaaaaacg cgactggaag cgacgcttga agcgcgtcgc agccaggaga tggactactt
     2641 catggaagta tttgttccca gcttccatct ttcgctcgat gagattagcc aggattcacc
     2701 gctgtaagca gccggagtct gcgtcgcatc aggcaataag cgccggatgc gacatcaggc
     2761 tcttgtcaaa acggaaacag catcgggatc atcaccacac aaaccgccat cacgataatg
     2821 gtgaacggta cccccaactt cacaaagtca ctgaagctgt aatttcccgg acctaaaacc
     2881 agtgtgttaa caggtgaaga aaccggtgtc ataaaggcgg cggatgctgc catcgccacg
     2941 accatcgcga atggataagg cgacaccccc atcgttttgg cagcagccag cgcaatcgga
     3001 gccatcaaca ccgccgtcgc ggtattagag ataaatagcc caataaccgc cgacaagaca
     3061 aacaaacacc ccagcatcat atgtggcccg taaccaccgc caatgtccat cagccctttc
     3121 accgccagcg cgacacctcc cgttttctgt aatgccacag caaacggcat catcccaacg
     3181 atcaaaataa tgctcggcca gtgaatggat ttataggcgc tttcagcatc tatacagcgg
     3241 aatttcccca tcagcaggca ggcgatgata gcggcaacag gattaggaat ttcatctgtc
     3301 agcattaacg ccaccattag caccagacag aaaatggcat ggggtgcctg gctgtgcgcg
     3361 ggcgatgctt cactcacctc ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg
     3421 gccagcatac cgatcagttt ccagttaccc acaaccagga tgatatcgcc cagcagcaga
     3481 ggctcatccg ccagcgaacc ttccagcgcc acgccattgc gctttagccc caccacattc
     3541 agtccgtagc gggtacgaaa accaatttcg cgcaccgatt taccaatcag ttctgactca
     3601 ggaattaatg aaatctctgc catgcccaca tcaagggcct ggtcagaaaa atactcgccg
     3661 cgcagtacca tcggctccag caattgctca ctacaaaatt gccggagatc gacatcagcc
     3721 gcagacatat caataagcaa aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc
     3781 acgataacgc gacgaaaacg ccgccagcgt tcaacaccga tgacgttagc gccataacgc
     3841 tcacgtaatt tgagatcatc cagccgttga cctatcattg gcgatccggg gcgaatagcc
     3901 agacgtcgcg cacgcccggt cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc
     3961 gtccagcctt cgcgctgcgg ggtctgggta tcccctttca gcatgaaacg catcactaac
     4021 atatacaaaa tacccagcac cagcacgacc aggccaatag gtgttacgct aaagaaactg
     4081 aagccgtgat agccttcacg cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc
     4141 accagcgtca tcatgccgct aatcagcccg gcaaaactta acggcatcat cagacgtgac
     4201 ggcgaagttt gcatacgcat ggcaacgctt aacaccacgg gaataaagat agcgacaacg
     4261 ccggttgaac tcataaacgc gccaagcccc gcgacggtca gcatcaacaa aaccaacatt
     4321 ttgatttcac tgttgcccgc cactttgacc agccatgttc ccattacggt ggcaacaccg
     4381 gtacggacca aaccatcgcc aataataaac aaggcggcaa tcaggacaac gttaggatca
     4441 gaaaagccgg aaaatacttc ggggaccgtc agcgttccgc ttaatgcaaa cgcgacaata
     4501 acaaacaaag cgaccgcatc catacgcact ctgcccgtcg caaacaagac gatggcaacg
     4561 gccagtaatg aaagaaccca aatcaattca ccgttcacaa cttatccttg ttaattgagg
     4621 gggatgactt gattctgcca taaaaaagcc ccgacgagac ggggctaaat catgatcagg
     4681 tgtttcactg aataataaca tcgccatttg gctgtttggt cacagtaatt tgctccagac
     4741 tgtggaggac caaatcgacc tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa
     4801 tgacatggca acccgccgcc aggccagaaa gcacgccagc gggagcatct tccaccacca
     4861 cacactcctg cggcgcaagc cccagcagct gcgcacctaa cagatacgca tcaggttctg
     4921 gttttccgcg cttcactcgc tcagcggtta caaacacctc tggtgcggga agcccagcta
     4981 ttttatggcg cgctcgcgct accggcatgg agccagaagt cacaatggcc cacggaatac
     5041 ctgctttatt caaatgattg agtaaggcga ttgcccccgg aagcgcggta ataccttcgg
     5101 tttccgtggc ctcgatgtgc tccagacgcg taaactcggc ggcaatatca gcctcggatt
     5161 tgcccgccat aaaatggcgc aaagaggtga tcgcctgttt accgtgaatg aaagccagca
     5221 cctcttccgg cgctaaccca tgacgtctgg cccagttgct ccacgcccgt tctaccgcag
     5281 gcagggaatc caccagcgtt ccatcaagat caaacagaaa acctttgcac cgcacgcggg
     5341 cctcctcagg cgttgataat ttgattaatt tcgttggcgc tcaaatggta ctgacgcggg
     5401 caggcatgcc acacattaag catgcgctga tatttttccc acattggcgt ctgggcgtta
     5461 aaaccgtgag ttccggcgtc aaaatgggta tagcgccctt ccacattaac cataaagcgg
     5521 acataaccga ggtaacgtgc ttcagtggcg gcgtcaaagc cgaggaaggt gacacgacgt
     5581 tcatcgatgg attgctggtc ctgcaaatta gaccaggaaa catgcaacgc atgatacatc
     5641 tccataatgt cgatgatagt gcggcaggtt tcttctttca gctcgccaaa ctcgcgatcc
     5701 aattcacgca tctgtaatcc gtaaccacgc tcaataattg tttgcaggcg acggtaacgt
     5761 tcagcatttg ccggatcgag catagtcatc atcttgtact gattagacaa aataagacgt
     5821 tgcgcgttgg tcatttccat tgttgactcc tgtatcacta tactacggtg aaaaaaaaga
     5881 aggctgagta tgccttcttt tatatgcgta atcaggggtc aattacaaat catcaaggaa
     5941 agttttatcc agttgtttga aggcgcgctt aagcgtgtca gctaatgcct ggtaatcagg
     6001 cttgccttca acgggtgcca acacctgtcc agactcctgc aatttaccgc gaacttcata
     6061 aaaccagtta aggattgcag ggggtaatgg cgttacagaa cgcttgccca gccaccacaa
     6121 tccctgcatg ggtaaactta aggcgaacag cgcagtggca actgccggcc caagctgacc
     6181 gcccagggca atctgccagc agagagtaaa tacggcgatc ggcggcataa aacggatcgc
     6241 ataacgcgtc atcttgataa cgcgattttc gacaaagacc ggggcaaggc gtttttccag
     6301 cggccacgtc tttgagtaat gctgtccccg gcgaaacaag ctaaaaaaat taacagaacg
     6361 attatccggc gttgacatgc ttcacctcaa cttcacatat aaagattcaa aaatttgtgc
     6421 aaattcacaa ctcagcggga caacgttcaa aacattttgt cttccatacc cactatcagg
     6481 tatcctttag cagcctgaag gcctaagtag tacatattca ttgagtcgtc aaattcatat
     6541 acattatgcc attggctgaa aattacgcaa aatggcatag actcaagata tttcttccat
     6601 catgcaaaaa aaatttgcag tgcatgatgt taatcataaa tgtcggtgtc atcatgcgct
     6661 acgctctatg gctccctgac gtttttttag ccacgtatca attataggta cttccatgtc
     6721 gagtaagtta gtactggttc tgaactgcgg tagttcttca ctgaaatttg ccatcatcga
     6781 tgcagtaaat ggtgaagagt acctttctgg tttagccgaa tgtttccacc tgcccgaagc
     6841 acgtatcaaa tggaaaatgg acggcaataa acaggaagcg gctttaggtg caggcgccgc
     6901 tcacagcgaa gcgctcaact ttatcgttaa tactattctg gcacaaaaac cagaactgtc
     6961 tgcgcagctg actgctatcg gtcaccgtat cgtacacggc ggcgaaaagt ataccagctc
     7021 cgtagtgatc gatgagtctg ttattcaggg tatcaaagat gcagcttctt ttgcaccgct
     7081 gcacaacccg gctcacctga tcggtatcga agaagctctg aaatctttcc cacagctgaa
     7141 agacaaaaac gttgctgtat ttgacaccgc gttccaccag actatgccgg aagagtctta
     7201 cctctacgcc ctgccgtaca acctgtacaa agagcacggc atccgtcgtt acggcgcgca
     7261 cggcaccagc cacttctatg taacccagga agcggcaaaa atgctgaaca aaccggtaga
     7321 agaactgaac atcatcacct gccacctggg caacggtggt tccgtttctg ctatccgcaa
     7381 cggtaaatgc gttgacacct ctatgggcct gaccccgctg gaaggtctgg tcatgggtac
     7441 ccgttctggt gatatcgatc cggcgatcat cttccacctg cacgacaccc tgggcatgag
     7501 cgttgacgca atcaacaaac tgctgaccaa agagtctggc ctgctgggtc tgaccgaagt
     7561 gaccagcgac tgccgctatg ttgaagacaa ctacgcgacg aaagaagacg cgaagcgcgc
     7621 aatggacgtt tactgccacc gcctggcgaa atacatcggt gcctacactg cgctgatgga
     7681 tggtcgtctg gacgctgttg tattcactgg tggtatcggt gaaaatgccg cgatggttcg
     7741 tgaactgtct ctgggcaaac tgggcgtgct gggctttgaa gttgatcatg aacgcaacct
     7801 ggctgcacgt ttcggcaaat ctggtttcat caacaaagaa ggtacccgtc ctgcggtggt
     7861 tatcccaacc aacgaagaac tggttatcgc gcaagacgcg agccgcctga ctgcctgatt
     7921 tcacaccgcc agctcagctg gcggtgctgt tttgtaaccc gccaaatcgg cggtaacgaa
     7981 agaggataaa ccgtgtcccg tattattatg ctgatcccta ccggaaccag cgtcggtctg
     8041 accagcgtca gccttggcgt gatccgtgca atggaacgca aaggcgttcg tctgagcgtt
     8101 ttcaaaccta tcgctcagcc gcgtaccggt ggcgatgcgc ccgatcagac tacgactatc
     8161 gtgcgtgcga actcttccac cacgacggcc gctgaaccgc tgaaaatgag ctacgttgaa
     8221 ggtctgcttt ccagcaatca gaaagatgtg ctgatggaag agatcgtcgc aaactaccac
     8281 gctaacacca aagacgctga agtcgttctg gttgaaggtc tggtcccgac acgtaagcac
     8341 cagtttgccc agtctctgaa ctacgaaatc gctaaaacgc tgaatgcgga aatcgtcttc
     8401 gttatgtctc agggcactga caccccggaa cagctgaaag agcgtatcga actgacccgc
     8461 aacagcttcg gcggtgccaa aaacaccaac atcaccggcg ttatcgtcaa caaactgaac
     8521 gcaccggttg atgaacaggg tcgtactcgc ccagatctgt ccgagatttt cgacgactct
     8581 tccaaagcta aagtaaacaa tgttgatccg gcgaagctgc aagaatccag cccgctgccg
     8641 gttctcggcg ctgtgccgtg gagctttgac ctgatcgcga ctcgtgcgat cgatatggct
     8701 cgccacctga atgcgaccat catcaacgaa ggcgacatca atactcgccg cgttaaatcc
     8761 gtcactttct gcgcacgcag cattccgcac atgctggaac acttccgtgc cggttctctg
     8821 ctggtgactt ccgcagaccg tcctgacgtg ctggtggccg cttgcctggc agccatgaac
     8881 ggcgtagaaa tcggtgccct gctgctgact ggcggctacg aaatggacgc gcgcatttct
     8941 aaactgtgcg aacgtgcttt cgctaccggc ctgccggtat ttatggtgaa caccaacacc
     9001 tggcagacct ctctgagcct gcagagcttc aacctggaag ttccggttga cgatcacgag
     9061 cgtatcgaga aagttcagga atacgttgct aactacatca acgctgactg gatcgaatct
     9121 ctgactgcca cttctgagcg cagccgtcgt ctgtctccgc ctgcgttccg ttatcagctg
     9181 actgaacttg cgcgcaaagc gggcaaacgt atcgtactgc cggaaggtga cgaaccgcgt
     9241 accgttaaag cagccgctat ctgtgctgaa cgtggtatcg caacttgcgt actgctgggt
     9301 aatccggcag agatcaaccg tgttgcagcg tctcagggtg tagaactggg tgcagggatt
     9361 gaaatcgttg atccagaagt ggttcgcgaa agctatgttg gtcgtctggt cgaactgcgt
     9421 aagaacaaag gcatgaccga aaccgttgcc cgcgaacagc tggaagacaa cgtggtgctc
     9481 ggtacgctga tgctggaaca ggatgaagtt gatggtctgg tttccggtgc tgttcacact
     9541 accgcaaaca ccatccgtcc gccgctgcag ctgatcaaaa ctgcaccggg cagctccctg
     9601 gtatcttccg tgttcttcat gctgctgccg gaacaggttt acgtttacgg tgactgtgcg
     9661 atcaacccgg atccgaccgc tgaacagctg gcagaaatcg cgattcagtc cgctgattcc
     9721 gctgcggcct tcggtatcga accgcgcgtt gctatgctct cctactccac cggtacttct
     9781 ggtgcaggta gcgacgtaga aaaagttcgc gaagcaactc gtctggcgca ggaaaaacgt
     9841 cctgacctga tgatcgacgg tccgctgcag tacgacgctg cggtaatggc tgacgttgcg
     9901 aaatccaaag cgccgaactc tccggttgca ggtcgcgcta ccgtgttcat cttcccggat
     9961 ctgaacaccg gtaacaccac ctacaaagcg gtacagcgtt ctgccgacct gatctccatc
    10021 gggccgatgc tgcagggtat gcgcaagccg gttaacgacc tgtcccgtgg cgcactggtt
    10081 gacgatatcg tctacaccat cgcgctgact gcgattcagt ctgcacagca gcagtaatct
    10141 cgtcatcatc cgcagctttg cgctgcggat atctgaaccg gaaataatca ctatttccgg
    10201 ttttttattc tcttaatctg cattaatcct ttctgattat cttgcttaac tgcgctgcat
    10261 caatgaattg cgccatttca ctttgcatac ttaccacttt gttttgtgca agggaatatt
    10321 tgcgctatgt ccgcaaccac tgaatccaaa ccaacaagaa gatgggcaat gcccgatacg
    10381 ttggtgatta tcttttttgt tgctatttta accagccttg ccacctgggt agttccggtg
    10441 gggatgtttg acagtcagga agtgcagtat caggttgatg gtcaaacaaa aacacgcaaa
    10501 gtcgtagatc cacactcatt tcgcattctg actaacgaag caggcgaacc tgagtatcac
    10561 cgcgtacagc tgttcacgac gggcgatgaa cgcccgggcc tgatgaactt cccgtttgaa
    10621 ggattaacct caggatcgaa atacgggaca gctgttggca tcatcatgtt tatgctggtg
    10681 attggcggcg cgtttggcat tgtgatgcgt acaggaacca ttgataacgg tatcctggcg
    10741 cttattcgcc atacccgcgg gaatgaaatt ctctttattc ctgcgctgtt tattctgttt
    10801 tcacttggcg gcgcggtatt tggtatggga gaagaggccg tcgcctttgc cattatcatc
    10861 gcaccgctaa tggtccggct gggctatgac agtattacca ccgtcctggt gacctatatt
    10921 gccacgcaaa tcggttttgc cagttcgtgg atgaacccgt tttgtgtggt cgttgctcag
    10981 gggattgccg gcgttccggt gctttctggc tccgggttgc gcatcgtggt gtgggttatc
    11041 gccactctga ttggcctgat ctttaccatg gtgtacgcct cacgagtgaa aaagaatcct
    11101 cttctgtcac gcgtgcatga gtccgaccgc ttctttcgtg aaaagcaggc tgatgttgaa
    11161 caacgtccgt ttacctttgg tgactggctg gtattgattg tcctgaccgc cgtaatggtc
    11221 tgggtgattt ggggcgtgat cgttaatgcc tggtttattc cagaaattgc cagccagttc
    11281 ttcaccatgg gtctggtgat tggcatcatc ggcgttgttt tccgccttaa cggcatgacg
    11341 gttaatacca tggcttcatc ctttaccgaa ggggcgcgaa tgatgatcgc ccctgccctg
    11401 ctggtgggtt tcgccaaagg gattttgctg ctggtcggta atggtgaagc aggtgatgcc
    11461 agcgtgttaa ataccatcct caacagcatt gccaatgcca ttagcggtct ggacaacgct
    11521 gtcgcggcct ggtttatgtt gctcttccag gcggtattta atttctttgt gacatccggt
    11581 tctggtcagg cggcgttaac catgccgtta ctggcaccgc ttggcgatct ggtcggtgtt
    11641 aaccgtcagg ttaccgtgct ggcattccag tttggtgatg gcttcagcca catcatttac
    11701 ccaacctcag cttcgttaat ggcgacgctc ggtgtttgcc gggtggactt ccgtaactgg
    11761 ctgaaggtgg gcgcgacact gcttggactg ctgtttatta tgtccagcgt cgtggtgatc
    11821 ggcgctcagt tgatgggcta ccactaaaaa tgttaagagc cgcaattgcg gctctttttt
    11881 cattctgccg tttcagtctc tacggcttca tttttggcat tgcgcttcat ccacaacgcc
    11941 agcgctttca gcgagtctgg agtgaactca tcgcagcgtg cggtgatttc ttccggcgtc
    12001 agccagcaaa cttcactgac ttcatcttcc tgtagggcga agggaccgtg agagacgcag
    12061 ctgaacaatg cgccccagac acggcaattt ttatcttcga aatagaactg cccgtgctca
    12121 gcaaagggga caccggcaat gcccaactct tcttccgctt cgcgacgcgc ggattccagc
    12181 agttgctcat cggcctggac cacaccgcct gcggtcgcat ctaacatgcc gggtaaaaag
    12241 tcttttgtct cggtacgacg ctggaccaga attttgccca tgccatcatg cacgacgatg
    12301 taagttgcac gatgacgcag acactgtgcc cgcatttgtt cccggctggc ttgtgcaatg
    12361 acttcgttct cttcattgac aatatccacc cattcagtac ttgccaaacg acgctgttcc
    12421 attatcagga aaccttcttt ttctggcgct cttacggcgc attttgagtt gtgggtaaat
    12481 tacggattaa tcgcgacctg cgcaatgata ctttgatcat tgagtgcgat tacactaagt
    12541 acgtcattat ccagcatgcc ataactcgcc ggattaccgc ctttcggaat actcaccgag
    12601 ccggggttga agtggaaaat ctccccccgc tgttctgcca ctggcagatg ggtatggccg
    12661 tacaccagca catcgttctg gtttaaagca ggcagatttt ccgggccaaa aagatgacca
    12721 tgcgtcaaaa acagacgttg tttttccagt aatacctgtt gccacggcgc ggttatcggg
    12781 aaatgcagca gcatttgatc cacttcgctg tcgcagttgc cgcgcacagc gataacctta
    12841 tgtgccactt cattaagccg ttcagcgact ttggctggcg cgtaaccctc cggtaaagca
    12901 ttacgcgggc cgtgattcaa cacgtcgcca aggatcacca gccactgggc accgctttgg
    12961 gcaaacaact ccagaacacg ttccgtcgcc ggtaacgacc catgaatgtc cgatgcaaac
    13021 atcagtttca tcactcactc ctcgtcgaaa aaacgtaagc tatgatactg gattctgtcg
    13081 ttgctatcag ccagattgct tcgccgaaag tgcaataaaa cgctggacag acgctcgctg
    13141 ccactggaat gtcgcataat ccaccaggcg ttctggcacc tcatcgccat gtagtaccag
    13201 gcggttaatc attagcgcca gatcagtatc agcaatgcac cattcaccaa ataaattcgg
    13261 ctgacccagt actaacaaat gttctgccat cgcaaacagt ttctctgcac tggcttttcc
    13321 ctcggccgtt agtggcgctt ttttcgcccc cgcaaagaca acatccgtcg gacgctcttc
    13381 gcggatgggc atcagatcgc tgcgcagcca ggcctgaatc tgtcgtgcac gcgcacgatt
    13441 ttctaaatca agcggataaa tacgttccca ggtcggtggc gcaaatcgat cttccagata
    13501 ctccgcaatg gcagaagatt cactcaactc aaaatcatcg atttgtaata acggcacacg
    13561 gcgtgtctga ccgtaacctt gccacgtcgg ctgcaaatgt tcaccgctgt cgaggtcgat
    13621 ggtcttgata tgaaacgaca ggcctttttc ctgcaacgcc acccaggcgg ataacacata
    13681 aggggagaaa aagtgggcat ctgaccaaag cgtgatagcg ggtttactca ttatgtcctc
    13741 ggcaataaag ggggctggct atcaaactat agagcctttt tgtcactgtc acgtgacgaa
    13801 tactcacgta agtcgccgcg ctttctatac ttgtggtgtt cagatgatta aacaaccgga
    13861 gctgcaatga tcgatctcta tttcgccccg acacccaatg gtcacaaaat tacgctgttt
    13921 ctcgaagaag cagagcttga ttatcgcttg attaaggtag acctggggaa aggcggtcag
    13981 tttcgtccgg aatttttgcg catttcgccc aacaacaaaa ttccggcaat tgttgatcat
    14041 tctcctgccg atggcggcga accgttaagc ctctttgagt ctggtgccat tttgttgtat
    14101 ctggctgaga aaacaggact ctttttgagt catgaaacgc gtgagcgcgc cgccacatta
    14161 cagtggttat tctggcaggt aggcggactg gggccgatgc ttgggcaaaa tcatcatttt
    14221 aatcacgcag ccccccaaac cattccttac gctattgaac gttatcaggt tgaaactcag
    14281 cgtctttacc atgtactgaa caagcggctg gaaaactcgc cctggctggg aggcgagaac
    14341 tacagcattg cggatattgc ctgctggccg tgggttaatg cctggactcg ccagcgaatt
    14401 gacctcgcaa tgtatccggc agtcaagaac tggcatgagc ggatccgttc gcgccctgcc
    14461 accgggcagg cactgctaaa agcacaactc ggtgatgagc gttcggatag ttaacagaaa
    14521 caggttctcg tgtattattt catcctaagt aaaacaacgg agaacctgca atggcacaac
    14581 ctgccgctat tattcgtata aagaaccttc gtttgcgtac gtttatcgga attaaggaag
    14641 aagaaattaa caaccgtcag gatattgtta tcaatgtgac gatccactac cccgccgata
    14701 aagcgcgcac tagcgaagat atcaacgatg cgctgaatta tcgcaccgta acgaaaaaca
    14761 ttattcagca tgtagagaat aaccgtttct ctttgctgga aaaattaact caggatgtgc
    14821 tcgatatcgc acgtgaacat cactgggtga cgtatgctga agtggagatc gataaactgc
    14881 acgcgctgcg ctacgccgat tcggtatcca tgaccttaag ctggcagcgt taaccgccat
    14941 atcgggaggc tgcatgaaca tagtgatcac cggagggacg ggattaattg gtcgccattt
    15001 gattccgcgt ttgctggagc tgggccatca aatcacggta gtgacgcgta acccacagaa
    15061 agccagttcc gttctcggcc ctcgggtgac gctatggcaa gggctagccg atcaaagcaa
    15121 cctcaacggc gttgatgcgg taatcaacct ggccggagaa ccgattgctg ataaacgctg
    15181 gactcacgag caaaaagagc gtctctgcca aagccgctgg aatatcacgc aaaaactggt
    15241 cgatttgatt aatgccagcg acacgccgcc gtcggtactc atttccggct cggcaacggg
    15301 ctattatggc gacttaggtg aagtggtggt taccgaagag gaaccgccgc ataacgaatt
    15361 tactcataaa ctctgcgccc gctgggaaga gattgcctgc cgggcgcaaa gtgacaaaac
    15421 gcgagtgtgc ctgctgcgaa ccggcgtggt actggcaccg gacggcggta ttctcggtaa
    15481 aatgctgcca ccgtttcgtc ttggcctggg cgggccgatt ggttccggtc ggcagtatct
    15541 ggcctggatt catattgatg atatggtcaa cggcattctc tggctgctgg ataacgacct
    15601 gcgcgggcca tttaatatgg tttcgcccta cccggtacgc aatgaacaat ttgcccatgc
    15661 gctcggtcaa gcgctgcatc gcccggccat tttgcgcgtc cccgcaaccg ccattcggct
    15721 gttaatgggc gaatcttcaa tactggtatt aggtggacaa cgcgcgctgc ctaaacggct
    15781 ggaagaagcg ggttttgcgt ttcgctggta cgatttagaa gaggcgctgg cggatgtcgt
    15841 tcgctgatgt ggtttacagc aaacatccgc cagttaactc ccggtgttac aggattagtg
    15901 gctttgcgcg ataagatcgt ctggtgaaag tcgggtcacc atcataacta actctctgtc
    15961 taaacctcta tccagcatct cctgagcaat acgcagggct tcttcgtgtt tgccctgcat
    16021 tgcgccttct tcacgtaatc tgtcagcaat ggtcatcagt ttctcctttt cttgtggtgc
    16081 gcgttctgtt atctcaccaa taaatgcacg gaaacgctgg gcatcccctg tttgtaatac
    16141 gtaattaaac agggctttta gctgtctgtc attagtgttc cctgtaacta gcagcgaaac
    16201 aatttggtcg actaatccca acagatcgcg ctgacgaata tgtttctgaa ttaactccaa
    16261 cagcgccatt ttgcggtgtt gcataatctc gtcatccggc accacggtaa tatccaccaa
    16321 cggaaacgct gatgaatata ttttgcgggc tatagcaggc tcggcaaatt catcaagcca
    16381 gcagagtgaa taaggataag gacttctgca accatgataa aacagcatcg ggatcaccaa
    16441 tggaagctct ttatagcccg catcaagatg gttttgcatt gccgcaatgg aataacgcat
    16501 catgcgaaaa gccattaatt cttccggctt actttggtgc tctatcacta cataaatata
    16561 acccactccc tcctgcgttt tcacagacca caagaggtcg gaataatatt gccgcaggtc
    16621 gtcatcaata aaactgtttg gttccagttt aagcgtcgtt aaatcacaca gtttgcgcag
    16681 cggcgcggga agatgaatat caataaaatc ccgcgcggtg tctggatggc gtaaaaaaga
    16741 tttaaatacc gcatcatgcg gcgtggaagt tgtcgatatt gtcatggcgt tccgtcaccc
    16801 ctcaaattta agatgacgcg acaataacca cgattaacac ccaatgcagc aggcagtttt
    16861 tatctttgga cagcgaattc agaagtattt ttagcggact gcaactttta catttcagcg
    16921 gaagctgact cacaaattcc ccatgaaaat actccggcag cgttagccac cggagaggaa
    16981 attatttcag cgatccctta aggaactgtt gcagacgagg gctttgcggg ttgccaaata
    17041 actgctccgg cgcgccctct tcttctattt ttccctgatg gaggaaaatg acgtgagtag
    17101 aaacatggcg agcaaagccc atttcgtgag tcactaccac catggttttc ccctcttctg
    17161 ccagttgctg cataatacgt aacacttcgc ctaccagttc aggatcgagc gccgaggtag
    17221 gttcatcaaa cagtaaaact tccggttcca tcgccagcgc ccgcgcgata gaaacacgct
    17281 gttgctgacc gccggaaaga tgcaccggat atttcccctg cgcacgttcg tctatcccga
    17341 ctttcgccag atacttcacc gcccgctcgc gcgcttcctg cttgctcagg cccaacacct
    17401 gaatcggcgc ttccatgacg ttttccagca ccgtcatatg gctccagaga ttgaagtgct
    17461 ggaataccat cgtcaggcgt gtgcgcagta agcgcagttg atttttatcg gcgactttga
    17521 gttgaccatc tttgtcgcgc accagattga tcgtctggcc attgaccacg atcgaccctt
    17581 cactcggttt ttcgaggaag ttaatgcagc gcagaaaggt acttttcccc gatcccgacg
    17641 atccgatgat gcttattaca tctccggcat tcgcttgcag tgagacccct ttcagcactt
    17701 catgttcgcc gtagcgtttg tgcaaatcga taacgtttaa tttattctcg gacatcgtat
    17761 tctcagtgcg ttgaagaagg tttcacatgc tgcaaccagc gtttttccgc tctgcgaaag
    17821 aggctgatca ggacataaga gatgattaaa tagagcaccg cggcaatgcc gaaggcggta
    17881 aaaggttgat acgtggcggc gttaatatcg cgggctattt tcagcagatc cggcacagtg
    17941 gccgtaaacg ccagcgcagt agagtgcagc atcaggatca cttcgttgct gtatgccggt
    18001 aacgcaatac gcagcgcaga aggcaaaata atgcaacgat acattttaaa agtcgagaag
    18061 ccataggctc tggcggcttc aatttcccca tgcggaaccg aacggattgc cccagcaaaa
    18121 atctcggtag tgtaagcgca ggtgttaagc gtcagcgcca gcacggtaca gttcaggcca
    18181 ctgcggaaga aagcgttaag gaattcggtt cccttaacaa tctcaagcgt gtacatgccg
    18241 gaatagaaca ccagcaactg aacatacagc ggcgtaccgc gaaaaatata ggtaaataac
    18301 cagattggaa actggatgta tttattactg gagacacgac caatcgccag aaacagcgcc
    18361 aggactccgc ctatcactac cgacaaaata agcagccaca gagtgatcgc cacaccagta
    18421 aagcgataac cgtcggtcca cagcagcggt ttccagtatt catgtaagat ttcgatcaca
    18481 ggtcagccct cttcacaccc acggagtagc ggcgctcaag gaacagcagc acaccattgg
    18541 aaacagtggt gaaaaccagg taaatcacgc cacagacgat ggcgaaatag aacggttccc
    18601 aggtactttt gcctgccagt tgggtggctt tgaccacatc ttccaggccg agtaacgaaa
    18661 ccagtgcggt agatttgagg atcacctgcc agttgttgcc aatgcctggc agggcgtaac
    18721 gcatcatcgc cggaaacatg atccgccgaa acacttgccc acgagtaaaa ccgaacgccg
    18781 tcgccgcctc tatatgccct ttcggcactg ccataaacgc gccacggaac gtttcggtaa
    18841 aataagcacc gtagataaaa ccgagagtaa taataccagc gaccatcgga tcgatatcaa
    18901 tctgcccgac gcccatcgcc tccgtcaccg tgtttagtgc aatctgcaaa ccgtagaaaa
    18961 tcagcagcat caacaccaag tccggcacgc ctcgaatcag cgtggtgtac ccttcgaaaa
    19021 tcagccccga aagccgattt tgcgagagct taccgccagc gccaattaaa ccgatgatta
    19081 cagcgagcac tacagaactg atagccagct ccagcgtgac gagcgcaccc tgtaaaataa
    19141 cacctgaaaa cccatacaac atgctgcctg tcctgtcgtg tgtggtgaat taccgcctct
    19201 tgtctccctc cggttacccg aagggagcgg gctttctttc accgatggcg attaaccgcc
    19261 ataaacatca aaatcgaagt actttttcgc taatttctcg taagtaccgt cagcgcgcat
    19321 ttcggcaaag gctttgttca gtgcctcgcg cagttcgtta tcttctttac gcaggcccat
    19381 gccggtccct acgccaaaca gtttttcatc tttaacagac gggccaccga atttgtaatc
    19441 tttaccgaca ggttgtttga ggaaaccttc gctggcagcg acctcatcct ggaacgcggc
    19501 atcaatacgt ccggcagtca ggtcagaata aatgttgtcc tgcccctgat acgagacgat
    19561 ttcaatgcct tttggtgccc aatgttcatt accgaacgtc tcctgggtgg tgccctgcaa
    19621 tacgcctacc cgtttgcctt tcagcgactc gactgtcggc tgaatgtcgg aatttttcgc
    19681 caccaccaaa cgagaatcgg cagcgtacag tttgtcggtg aaggctattt cttgctgacg
    19741 tttttccgta atggaaagcg atgacatgat ggcgtcaatc ttcttcgctt ttaaggacgg
    19801 gattaacgca tccagcggat tttcgacaaa cgtacattgc gtattgatgc gtttgcataa
    19861 ttcctttgcc agatcgatat cgaagccaac cagttcgcct tgtgaatttt ttgattcaaa
    19921 tggcgcatag gtcgggtcgg taccgatgcg gatgttttgc ggaatcgcag caaacgccgc
    19981 agttgcgctg gagaaggcca gaaccagaga gagcgatagc accagttttt tcataatgtc
    20041 ctcaactgac agtcttttta tagggaatat ttacaggttt gttacactta tcgtgccata
    20101 aaattgacca tcaaggcaaa atatcccgcc ccgttagacg tattattttt cattaaaaat
    20161 gcttaagtat gcatttcaat gcaccacgat agtgcaacaa acctggcgtg caccatgatg
    20221 atcattccat caggtacagc ttcccagcga cgtatcagtc accgtagaca ttaaagtcga
    20281 aatacttttt cgccatcttg tcgtaggtgc cgtcctgacg cagctcgcca agcgccttat
    20341 tgaaggcagc cgtcagttca gcatcatctt tacgtagccc tacaccggtg ccatcaccga
    20401 agtatttttt gtcttttact gatgagccag caaaggcgaa atctttacca gcaggttgct
    20461 tgaggaatcc ttcgctggca gcaacttcat cttgtaacgc agcatccaga cgtcctgcag
    20521 ccagatcgga atagaccaaa tcctggttgg cataggccac cacatccacg cctttgctac
    20581 gccaggtctc gttagcgtaa gcttcctggg ttgatccctg cagcacacca acatgtttac
    20641 ctttcagtga atccagcgtt ggctgaatcg gtgaaccttt ggcagcaatc aaacgagaat
    20701 ctgcggcgta cagcttgtcg gagaaggcaa tctcctgctg acgtttatcg gtaatggaaa
    20761 gcgacgaaat aatggcgtcg atttttttcg ctttcagtga ggggatcagc gcgtcaaagt
    20821 cactggcaac ccaggtacat ttcacctgca tccgtttgca catctcgtta ccgagatcga
    20881 tatcaaagcc aacaaaatca cctttagcat ctttcgacga gaacggcgca taggtggtat
    20941 cggttccgat acgtaccgtc tccggtagcg ccgcatagct ggaagccgct gcggagagac
    21001 cgactaacaa agacagagcg agaatcgact tcttcataca taaccctcaa gtgaaaatgg
    21061 cttttttatg ttgtgtattg tgttgtgtgt ttgcaggctc tttcatgcag gtcttatgcc
    21121 atcttgacga caacagtaac attcaacgtt aaatatgtta ataagacgtt gcattattgt
    21181 cctgaagttg aagatagcag gtatagcggt tgaatcgcag cgtttcgatt gaatggcaga
    21241 aacaaaatgt cgaggatttg atcgcggttg caaaattgcc ctgaaacagg gcaacagcgg
    21301 agttatgcgc cctgccagcg ggcaaagaga tcttcaggaa gggttattgc aaactggtca
    21361 agaacacgat taaccgtctg atttatcaca tcatcaaggg attgcgggcg atgataaaac
    21421 gccggaacgg gaggcataat caccgcaccg atttctgccg cctgagtcat taaacgcaga
    21481 tggcctaagt gcaatggtgt ttcacgcacg cagagcacca acgggcgacg ctctttcagc
    21541 accacatctg ccgcacgggt cagtaagcca tcagtatagc tatggacaat gccggaaagg
    21601 gttttgattg aacagggtaa aatcaccatc cccagcgtct ggaaagaacc ggaagagatg
    21661 ctggcggaaa tatcgcgcgc atcgtgcgtg acatcagcta atgcctgcac ttcgcgcagg
    21721 gaaaaatccg tttcgaggga taaggtctgg cgcgccgcct ggctcatcac cagatgcgtt
    21781 tcgatatctg tgacatcgcg cagaacctgt aataagcgca cgccataaat cgcgccgctg
    21841 gcaccgctga tgcctacaat gagtcgtttc ataaatagtt gccctgtcag acttgcaggc
    21901 agactttgca gtatttcggc gggagttgca agtcagggtg ccagaccggc accctcagcg
    21961 aaggcatcat ccttcgttat gcatttcgag attttccact tcgttctgac gttgcactgc
    22021 tttggcgtca tcattacgta acgtatcgag gaaatcgagg tagccctgat caacatcttt
    22081 ggtgacgtag acgccgttga acaccgagca ttcaaactgc tggatatccg gattttcagc
    22141 gcgaacggcg tcgatcagat cgttcagatc ctggaaaatc aacccgtcag caccgatgat
    22201 ctggcgaatt tcatcaactt cgcgaccgtg agcgatcagt tccgtggcgc tcggcatatc
    22261 aataccataa acgttcggga agcgaatttc cggtgccgca gaagcgaggt acactttctt
    22321 cgctccggct tcgcgtgcca tctcgataat ctgctcagaa gtggtgccac ggacgatgga
    22381 gtcgtcgacc agcaggacgt ttttatcgcg gaactcggcg cggttggcat tcagtttacg
    22441 gcgcacggac ttacgacgca gctgctggcc cggcatgata aaggtgcggc caacatagcg
    22501 gtttttaacg aagccctggc ggtacggttt gcccagaata cgagcaattt ccagcgcgat
    22561 atcacacgag gtttctggga tcgggatcac cacgtcgata tccagatctt cccattcgcg
    22621 ggcaattttc tcgcccagtt tcgtgcccat attcacacgc gcgctgtaaa cggaaatttt
    22681 gtcgatgaac gagtccgggc gagcaaagta tacatactca aacaggcacg gattgctgac
    22741 cggattgtca gcacattgac gggtaaacaa ctgcccttct tcagtgatgt aaatcgcttc
    22801 gcccggcgcg acgtcacgca ggaaatcaaa gcccagcgta tcgagcgcta cgctttcgga
    22861 agcgaccata tattctgtac ggttctcgtc aatatcacgt tttcccagta ccagcggacg
    22921 aatcccgttt ggatcgcgga aagcaaccat accgtggccg ataatcatcg ccacacaggc
    22981 atatgcgccg cggattaagc ggtttgtggc agcaatggca gcgaaaatat tgtcggcttc
    23041 cagcgggtag tggcggaagt tgtccagctc gctagcgaag atattaagca gaatttccga
    23101 gtcggaagtg gtgttgatgt ggcggcgttt ttcttcaaac agttttttac gcaactcgtg
    23161 agcgttggtc agattgccgt tgtgggcaag cgtaatgcca tacggagagt taacgtaaaa
    23221 cggctgcgct tcagaggcgc tggagctgcc agccgtaggg taacgcacat gaccaatgcc
    23281 catattgccc tgcaaacgct gcatatggcg agcttcaaat acatcgctca ccagcccgtt
    23341 cgctttacgc aaacggaagc agttattggc atctatggtg atgatgccgg cggcatcctg
    23401 accgcgatgc tgaagcaccg ttaaggcatc ataaatcgac tggttaaccg gcataacacc
    23461 ggcgataccg acaataccgc acatacgtct ttttcctcgt taagccacat ctcagagcac
    23521 ttacgctctg ggcaagaaac ttgacgagct ttgcagataa tcaaaaaacc atctgatgat
    23581 aaaactgaat tgcgggatca gctgtgattt gctccagtct tcgcttttcg acaccccggt
    23641 aaaggagtcg agaaagaaga gaatggcggc aacaatcaac acaccgcgca acgcaccgaa
    23701 acagacgccc agcacccgat cggtgcctga caaccccgtt ttctccacca actggcctat
    23761 cacgaagttc acgatagcac caacgatcag ggtagcgata aacagtaccg cgatggcaat
    23821 tccatttcga accagttcgt cttcaaagcc cgtaaaccag actgacaggt aagtgtagta
    23881 atgactggca acaaagaaag cacaacccca tgtcaccagc gataacgctt cacgaacaaa
    23941 gccgcggatc aggctaacca gagaggaaaa agcaatcacc gcgattatgg cgtaatcaat
    24001 ccagaccata tgtgtcccac gattttacgc cctgtcatcc tgttcggggc gcattctaac
    24061 agaaaaagaa aacgtttgcg tagggatttc cttcccgcgc atcaataaaa atggcgctga
    24121 aaaaatattc aacgccatcg actttttatg cctttgcggc atcgtgcaat gcgtgtcgga
    24181 tgcggcgtaa acgccttatc cgacctacgg ttctaacact gcccaggcct gataagacgc
    24241 gccagcgtcg catcaggcaa gactgtatta attcggcgta tagcccatta ccacgccact
    24301 taagccagaa agttgcttca actcacccag cgaacctttc agcttatctt tcgaggcatc
    24361 cggcccaacc agaatacggg taattttacc ctgcactggc gtggatggcg acgtataaac
    24421 ccgataaccg gcaccgcgca gcttaccgac aatctcattc actttatcgg catttttcag
    24481 cgcacccagt tgcacaacat aggctttacc cgttggcgct gctttttctt ccacgaccgg
    24541 cttcggttct ggcttcggcg caggtggtgc ttcaaccttc ggtttaggcg gctccaccgg
    24601 tttcgacttc ggtggggcga ccggtgcagg ttccggttca aactcggtgt tattggctgc
    24661 aatagtggtc ggatcgagcg acggtgctgc ggcatcacct gcccgcacct cttccgctgc
    24721 gccttccggc ggctgcgtcg gcaacgcctg ggtggcggct ggcatcatat caggctcatc
    24781 gcgatcgccc gctttcggca ccagcgggat agccgcgaac tcatcctgat aatgtttttt
    24841 ctgcccgtcc agcagccctg gaagtacaat cacccccagc gccaccagca cgatcgtgcc
    24901 cactaaccga ttctgaaact tacttgccac cgcttctcct cgcgtcaatc acttccatga
    24961 catgtgcgac agtgtggaaa gaaccacaca ccagcacggt gtcttccgct ttagcgtccg
    25021 ccattgcggc atcccatgcc tgcgcaacgc tatcaaatga tttgccgtta cccaaatgct
    25081 caagcagttg ttctgccgtg gcaccgcgcg gcccttccag tggcgcacaa taccagtcat
    25141 caaccacgct tttcaaccag gccagagttc cggcaatatc tttatcatgt agcataccga
    25201 taaccgccag cacgcgcccg tttttcggta gcgctttcat acgcccggtg agatattccg
    25261 ccgcatgtgg attatgcgcg acatcaaaaa taacgcgtgg cgactcgctc acaatctgga
    25321 aacgtcccgg caaaattgcg ctggcaatcc cgtcgcgaat ggcattttca ctgacttcca
    25381 gcccgctggc acgcagtgcc gccagcgccg ttgcggcatt cggttgcggg acaagcggca
    25441 acggcagatt ttccagcgtg ccgtgagcat cgctaaacgc ccagtcatga tcggtgacgg
    25501 aatagttcca ctcaacgccc cgacgttgta acagtgcacc tttttcctgt gccacatcag
    25561 caatggtaga aggcatttcc ggctcaccga caattgccgg tttttcgctg cggaagatgc
    25621 ctgctttctc gcgaccaata ctttcgcgat ctggacccag ccagtcggta tgatccagcg
    25681 caatactggt tactaccgcg acatcggcat cgacaatatt ggttgcgtcc agacgaccgc
    25741 ccagccctac ttccagaatc accacgtcaa gttgtgcctg cttgaacagc cacaacgccg
    25801 acagcgtacc gtactcgaaa taggtcaggg aaatatcacc gcgtgccgat tcaatctccg
    25861 caaaagaggc ggtgtgggcc gattccggca attcctggcc ctgcacacgt acgcgctcgg
    25921 tataacgcac cagatgcggt gaactgtaga cgcccacttt gtaccctgcc gccatcagaa
    25981 tcgactccag cgtacggcag gtggtgcctt tgccattcgt acccgcaacg gtaaacacaa
    26041 atggcgctgg tttcagaacg ccaagacgcg ccgcgacctg gctcacgcgc tcaaggccga
    26101 gatcgatagt tttactgtgc aggttttcca gataagaaag ccacgaagcc agaggcgacg
    26161 cggcttgagg agtgcgtttg ataatcatgg tatccgctga ttcgttacgg tgagaatagc
    26221 aaaagggcag agccagtggc cctgccctta tcagttatca ggcctcaggt tcctgatccg
    26281 gtaccggggg taccactacg ccttcacgcg gcgcttcagg attcggcgct ggcagattca
    26341 tcaacttcgc cagaatgctc gccagtttca ggcgcatttc cggacgacgg acgatcatgt
    26401 cgatcgcgcc tttctcgatc aggaattcac tgcgctggaa tcctggcggc agtttttcgc
    26461 gaacggtctg ttcgataaca cgcggaccgg caaagccgat taacgctttc ggttcagcga
    26521 tgttgagatc gcccagcatg gcgaaacttg cagaaacacc gcccatcgtc gggtcggtca
    26581 gcacggagat gtacggcaag ccgcgctcct gcatttttgc cagtgccgca gaggttttcg
    26641 ccatctgcat cagcgacatc agtgcttcct gcatacgtgc gccaccagag gcggagaagc
    26701 agatcagcgg gcagttatct tccagcgcct gctcaacggc acgcacgaaa cgtgcaccca
    26761 caacagaccc cattgaaccg cccataaagg cgaactcgaa tgccgcagcg acaaccggca
    26821 ttccatacag agtgcctttc atcaccacca gcgcatcttt ttcgccagtt tctttctgcg
    26881 cagatgccag acggtcttta tacttcttgg agtcacgaaa cttcagcaca tctttcggct
    26941 caagctcgct acccagctcc acaaggcttc cttcatctaa caggctatgc aggcgattac
    27001 gcgctgtcat acgcatgtga tggtcacact tcggacagac ctcaagatta cgttccagct
    27061 cagcgcggta taaaacctga ccgcagctat cacacttagt ccacacccct tcaggaatgc
    27121 tcgccttgcg ggtgggagta atgttgcttt taattcgttc aatccagctc attagggacc
    27181 tttctgtctg aacctggttc gatgccagtt ttatctttgg ggacgcataa tgccattttt
    27241 gcccccaaca gaccatgaat gttgcacatt aaaacataac agcccgaaac tttggataaa
    27301 aaagtggtcg aaccgcggag ttacttttta ttttgcggcg cgtgccgcag cgcgtttgtg
    27361 acgaataaat tcgaaaaccc caggcaatat tgaaacaaaa ataatgccaa caatcatcag
    27421 cttaaggttg ctctgaatga aggggagcgt gccgaagaaa taacccgcgt aggtaaaaat
    27481 cagtacccac aacagtgcgc cgatcacgtt ataagcggcg aaatgacggt acgacatgtg
    27541 gcccattccc gcaacaaacg gcgcgaacgt tctgacgatc ggtacaaaac gggcgagaat
    27601 aatcgttttg ccgccatgtc tctcgtaaaa ctgatgagtc ttatcaagat aactgcgacg
    27661 aaagattttc gaatccgggt tactgaacag tttgtcacca aataaccgtc caatggtata
    27721 gttcactgca tcaccgacaa tagcggcaat caacataagc accaccatca tatggacatt
    27781 gagatcgtta gtttccagcg acgccaatgc accagcaaca aacagcagcg aatcaccagg
    27841 caggaatggc gttaccacca gccccgtttc gcagaacaga atcaaaaaca gaatggcata
    27901 aacccagacg ccgtactccg cgaccagctc tgccagatgc acgtcaatgt gcaggataaa
    27961 atcgatgagg aaataaatca ggtccatatt tgcctatgcc ttgtactcgt catctttcag
    28021 gctgtaacta cgttggctac agcctgaaat gttccgagca ttattctcgt tagtccgcca
    28081 gaaatagcgg gcccattggc ggttttggaa gatcataccg gtcagggtaa tccaccgcga
    28141 ccagatacag cccttccgct tttgccgttg ctgccgcccg cgttctgtcc tttgccgcca
    28201 gcaactctgc tatccagctc tccggctggt tgtgggcacc gacttccatc aggctgccga
    28261 caatattcct gaccatatga tgtacaaagg cattcgcttt gatatctacc accacataag
    28321 gaccgtgacg catgacatta atatgcataa cgttgcgcca cggggttcgg gactggcact
    28381 gcaccgcacg gaacgaggtg aaatcattct cgcccagcaa gcattgcgca gcccgatgca
    28441 tccgttcagc gtccagcggt tcgtaaaaat gggttacccc tttactcagt accgccgggc
    28501 gcagccgatg attgtagatg atgtagcgat aacggcgagc cgtggcgcta aatcgggcat
    28561 gaaaatcatc aggtacagct ttaacccaac gcacggcgat gtcaccaggt aaattcgcat
    28621 ttacgcccaa ggtccacgcc gcgtctttgc gctgcgcggt tgtttcgaaa tgcaccactt
    28681 gcccggtacc gtgtacccct gcgtcagtac gcccggcgca gaagacggtg atgggttcgt
    28741 tcgccacctg ggagagcgcc ttttccagct tctcctgcac actgcggact tcattctgcc
    28801 gttgccagcc gtaatactta ctgccgtcgt actcaatgcc cagcgcaatt ttataaactg
    28861 gcagttgttg ctggtcggac attagtacag atactcctgc accagtttct cggcgatttt
    28921 tactgccatc agcgcgccgc caaagcgaac gttatcggcc accgaccaga actggacttg
    28981 ctccggcata ccgtagtcat tacgcacgca gccaacagaa agatgcggcg tacccgaagc
    29041 atcccctacc tgagtcggga attcgttctc ttcagagagc acaatatctt cgccctgagc
    29101 aaacgcatca cgcgcttctt ctgccgccag cggacgcagt gcttcaaagt tgaccatctg
    29161 ggcatgaccg tagaataccg gtgcctggac gacgcttgcc gaaatcatca gcccttcgtc
    29221 ctgcaggatt ttgcgtactt cgtcaacgat acgacgttct tcacgcacgc taccttcgct
    29281 atccggcaac aacggcagca tattgaacgc cagttgacga ccgaagaaat cttcttcgtc
    29341 aatcggaatg ccgttgagca atttcgcact ctgccccgct aacgcatcga ccgctttttt
    29401 gccctgggcg gaggctgaaa tcaggctggt aacactgata cgcgacaaac cgccctgatc
    29461 gattaacggt ttcagtgccg ccagcagctg gctggtcaga ctgtctggta cggcgatgac
    29521 attccggttg cggtaatcgg tcagtacaaa cgggtttact tccggcacca ccagcggtac
    29581 gtcgggttcg agagcaaaca gtccgctgct gtcgatcacc aggcaacctg agttggtcgc
    29641 ttcttcaacc caggcagcgg tagcttcttt gcctgcgaca aaaaatgcca gctgcgcctg
    29701 cgtccagtcg aattcagcgg catcctgcac ggtgattgtt ttaccaccaa agcgcagttg
    29761 ttcgcctgcg ctttcgttac gtgccagtgc ataaatttcc ccaaccggga actgacgttc
    29821 agccagcgtt tcaagcaggg cttcgcccac agcgccagtt gcgcccagga cggcaatgtt
    29881 ccagccttca gacatggtgg tttactccag aaatagcaaa gctccctgcc aatgttacag
    29941 cagagagcat gaagaagaga ttaacgtgcc ggatgatgaa cggcgttaaa acccagttta
    30001 cacagcaatg atgccgcact ggcgtcatca caaattacat acagagatga ccattcacgg
    30061 cgctcaagat agtttttgcg cagtttatcg aactcacccg gtatcccggc gactttacgc
    30121 agcggtgcgt catcgcggcg cacatcatac accaaatgca ccagcctttt cagcgtcggt
    30181 tgatcgagcg ggccatgcag cgtaatgcga ccaaactctg gcgcgggcag taatgtatcc
    30241 agcgcaacgt gctgttcatg cccaataaac ttgctatagg cttcaaacac ttgcgtagta
    30301 ccgcgtgctt taccttccag ggtataacct gcgatatgcg gcgtgccgat atccactttt
    30361 gtcagcaact ccacgttaag ttccggttcg ccttcccaga catccagcac tacgcttaac
    30421 ttctggcctt cattcaggca ggtcagcaac gcagtattat cgacgactgc gccacggcag
    30481 gcgttaatca gaatcgctcc cggcttcagg ctacggatca gtttttcatc cgccagatgc
    30541 agcgttttgt acggcccatc tttgaagagt ggcgtatgga aagtcagaat atccgcatgc
    30601 tgaactaatt catccagcga gcggaaatca ccctcatccc cacggtcagc gcgaggcgga
    30661 tcgcaaagca atgttttaat ccctaacgct tccagtcgcg cctgcaatcg acgtccaacg
    30721 ttaccaacgc ccacgatccc caccgtacgg tcgtgcagtg aaaatccatc gcgttcggca
    30781 agcatcagta gtgaggagaa aacatattcc accaccgcaa tcgcattaca gccaggtgca
    30841 gcggaaaaac caattcccgc ctgcttcaac catgcttcat cgacatggtc ggtccccgca
    30901 gtggcagtgc caacaaattt aatgggtttt cctgccagca aagattcatt cactttcgtg
    30961 accgaacgca ccatcagcgc atccgcgtct gccagttgag caacggggat tggacgcccg
    31021 ggaaccgcgg tcacctcacc caaacggcta aataagtcgc gggcataagg catattttca
    31081 tcaacaagga ttttcacgtt tgtgttacct gtatgagacg agagttaacc ggacaagtgt
    31141 gccataatct cgcggccagg catacttgcg aagatttcag gtataaggat acgtaatgat
    31201 acaacctatt tccggccctc ctcctgggca accaccaggt cagggagata acctgccgtc
    31261 tggcgcgggc aatcagcctt tatccagtca gcaacgtact tcgctggaaa gcttaatgac
    31321 gaaagtgacc tcactgacgc aacaacaaag agcagaactg tgggcgggta tcaggcacga
    31381 tattggtctg tcgggagatt caccgctgct ttcgcgtcac ttccctgccg ctgagcataa
    31441 tctggcgcaa cgtctgctgg ccgcgcaaaa aagccattct gcccgccagc ttttagcgca
    31501 attaggggag tatttacgtc tggggaataa tcgtcaggcg gtcacggatt atatccgtca
    31561 taactttggt cagacgccgc tgaatcagct ctcaccggag caattaaaaa ccattctcac
    31621 cctgttgcag gaagggaaga tggttattcc gcaaccacag cagcgcgagg cgaccgaccg
    31681 tcctttatta ccggcggagc acaatgcgct aaaacagctg gtgaccaaac ttgcggcggc
    31741 aacgggggaa cccagcaaac agatctggca atcgatgctg gaactttccg gggtgaaaga
    31801 tggcgagtta attccagcga aactgtttaa ccatctggtg acctggttgc aggcgcgcca
    31861 gacgttaagc cagcaaaata cgccgacgct ggaatcacta cagatggcgc taaaacaacc
    31921 tttagatgcc agtgaactgg cggcgttatc ggcatatatc cagcaaaaat atggcctttc
    31981 tgcgcaatca tcgctttctt ctgcccaggc cgaggatatt cttaatcagc tttatcaacg
    32041 gcgggttaaa gggattgatc cgcgtgatat gcaaccgctg cttaatcctt ttccaccgat
    32101 gatggacacg ttgcaaaata tggcaacgcg tcccgcgctg tggatactgt tagtcgcgat
    32161 tatcctgatg ctggtctggc tggttcgtta gccccgacga aatgacagta tcgtgacaat
    32221 aattcccagc accgcagaga tcgccccggc aagaaatacc gaagagtaac caaacgtggt
    32281 cgccagcatt cccgccagcg gcccggagac gccgagggcg atatcctgaa acgcggcgta
    32341 accgcccagt gcggtgccgc gaacttgtga ggggacgcgt ttaaccacct ccacgcccag
    32401 cgcaggaaag ataagcgaac atccggctcc ggttaacgcc gcgcccgcta atgcgaccca
    32461 cgcacctggg gcttgccaga gcagcaacaa gcccaccgtt tctacaagca gagagacaat
    32521 cgccactttc acgccgccaa aacggtccgg catccagcca aacatgacgc gcatcacgac
    32581 aaatgcgccg ccaaacgcgg taagagtaaa gcccgccatc gcccatcctt tgctggcaaa
    32641 gtagagcgaa acgaaagtcc cgataaccgc aaaaccaacg ccttgtagtg ccagccctaa
    32701 ccctggtttc cagataaacc cgacaacgct ccacagcgat ggacgttctc ccgccagggc
    32761 cggtactttg cgcactgtgc cgttacaggc ccacgccagt aagggtaata ccattgtggt
    32821 gatcgccagt gcggcaaaac cgtaatggct atgaatcaac aggccaagcg gagcaccaac
    32881 agcgagggca ccgtaaatcg ccattccatt ccatgacatc actttgccag agtgttttgg
    32941 ccctacgatg cctaaccccc aggtcagagc gcctgtcagt aactggcttt caccaaaccc
    33001 aagaatcaaa cgcccgatga ccaacagggc aaatttgaac ggtgcggaga caggcaaaat
    33061 cgccgccagc agcaacgcgc cgccagccag accacaagct aacatcccct gaagcgccga
    33121 acgttttgca ccatattgat cggccagtcg cccggcatag ccacgcgtca gcaccgtagc
    33181 cagaaactga atcccgacgg caatcccgac catggtattg ccatagccca gatcatgatg
    33241 aacaaacagc gggataaccg gcaacggcag ccctacggtc atgtaggtga gaaaaaccgc
    33301 aaaagcgatg cggaagagcg aaaaattggc agaagatcgt gtttcggttt ggcttacagc
    33361 agtcatgcat tactccagaa tgcagcgcaa ggcgaggagt atccccgtct catctctctg
    33421 gtttcagggt tacggtgcgt tggcaggatt taacgcgtac gtcttttcag aaggaaatcg
    33481 acaaagcggg aagtttgcct ggaacaggcg gcgattgtca atgatgtgaa aaagggaacc
    33541 atcaggttcc cttttgcgtt agtgccggag gccgcattag cgcgtaacgt cggatgcgac
    33601 gctggcgcgt cttatccgac ctactccgac tactccggcc tactgcgaat taatctttca
    33661 gcttgcgcat taccagcgtg gcgttggtgc cgccgaagcc gaagctgtta gacataacgg
    33721 tggtcagttc gcgatcggtc gtttcggtca cgatgttcag acctgcagcc tgctcgtcca
    33781 gctcttcaat gttgatgctc ggggcgataa agccgtgttc cagcatcagc agagagtaga
    33841 tagcttcctg tacgccagca gcgcccagag agtgaccggt catggctttg gttgcagaaa
    33901 tcgccgggct cttatcgccg aacacttcac ggatagctgc cagttctttc acgtcgccaa
    33961 ccggagtcga agtaccatga gagttcaggt aatcgattgg ggtatcaacg ccgtgcatcg
    34021 ccatcttcat gcagcgtact gcgccttcgc cagacggagc aaccatgtct gcaccatcag
    34081 aggttgcgcc gtagccaacg atttcagcat agatgtgagc accacgcgcc agcgcgtgtt
    34141 ccagctcttc aaccactacc ataccgccgc cgccagcgat aacgaaaccg tcacggtgag
    34201 cgtcgtaagt acgggaggct ttttccgggg tgtcgttgta tttagtagac agcgcaccca
    34261 tcgcgtcgaa ttcgcaggcc atttcccagc acagctcttc gccgccgcca gcaaacacga
    34321 tgtcctgttt gcccagttgg atctgctcta ctgcgttacc gatacagtgt gcggaagtcg
    34381 cacacgcgga gctgatggag tagttaacgc catgaatttt aaacggcgtg gcgaggcagg
    34441 cagaaacgcc ggatgccatc gctttggtga ccacatacgg gccaaccgct ttcaggccgc
    34501 gcgggccgcg cattgcgtca gcgccgaaca cctggaaacg cggggaaccg ccgccagaac
    34561 ctgcaatcag gccaacgcgc gggttattct ggtaagcttc cggagaaagg cccgcatctg
    34621 cgattgcctg ctccatagaa aggaatgcat aaatggatgc gtcgctcata aagcgcacaa
    34681 ctttgcggtc aatgaggcca gtggtatcca gttttacgtt gccccagacg tggctacgca
    34741 tgccggaatc cttcagctcc tgagagaaag tgatccctga acgtccttca cgcagagatg
    34801 ccaggacttc ctgctggtta ttaccgatgc tggaaacaat gcccaggcca gtaatcactg
    34861 cacgtttcat tcaatacctc tgtaagtcgc acatagagta agtttcgaat gcacaatagc
    34921 gtacacttgt acgccgaaca agtccgatca gccatttaat agagaaattt gcgcagcctt
    34981 acacacatcg ctaagatcga gccaccgcct gtaagacgag taacttacgt gaaacactac
    35041 tccatacaac ctgccaacct cgaatttaat gctgagggta cacctgtttc ccgagatttt
    35101 gacgatgtct atttttccaa cgataacggg ctggaagaga cgcgttatgt ttttctggga
    35161 ggcaaccaat tagaggtacg ctttcctgag catccacatc ctctgtttgt ggtagcagag
    35221 agcggcttcg gcaccggatt aaacttcctg acgctatggc aggcatttaa tcagtttcgc
    35281 gaagcgcatc cgcaagcgca attacaacgc ttacatttca ttagttttga gaaatttccc
    35341 ctcacccgtg cggatttagc cttagcgcat caacactggc cggaactggc tccgtgggca
    35401 gaacaacttc aggcgcagtg gccaatgccc ttgcccggtt gccatcgttt attgctcgat
    35461 gaaggccgcg tgacgctgga tttatggttt ggcgatatta acgaactgac cagccaactg
    35521 gacgattcgc taaatcaaaa agtagatgcc tggtttctgg acggctttgc gccagcgaaa
    35581 aacccggata tgtggacgca aaatctgttt aacgccatgg caaggttggc gcgtccgggc
    35641 ggcacgctgg cgacatttac gtctgccggt tttgtccgcc gcggtttgca ggacgccgga
    35701 ttcacgatgc aaaaacgtaa gggctttggg cgcaaacggg aaatgctttg cggggtgatg
    35761 gaacagacat taccgctccc ctgctccgcg ccgtggttta accgcacggg cagcagcaaa
    35821 cgggaagcgg cgattatcgg cggtggtatt gccagcgcgt tgttgtcgct ggcgctatta
    35881 cggcgcggct ggcaggtaac gctttattgc gcggatgagg cccccgcact gggtgcttcc
    35941 ggcaatcgcc agggggcgct gtatccgtta ttaagcaaac acgatgaggc gctaaaccgc
    36001 tttttctcta atgcgtttac ttttgctcgt cggttttacg accaattacc cgttaaattt
    36061 gatcatgact ggtgcggcgt cacgcagtta ggctgggatg agaaaagcca gcataaaatc
    36121 gcacagatgt tgtcaatgga tttacccgca gaactggctg tagccgttga ggcaaatgcg
    36181 gttgaacaaa ttacgggcgt tgcgacaaat tgcagcggca ttacttatcc gcaaggtggt
    36241 tggctgtgcc cagcagaact gacccgtaat gtgctggaac tggcgcaaca gcagggtttg
    36301 cagatttatt atcaatatca gttacagaat ttatcccgta aggatgactg ttggttgttg
    36361 aattttgcag gagatcagca agcaacacac agcgtagtgg tactggcgaa cgggcatcaa
    36421 atcagccgat tcagccaaac gtcgactctc ccggtgtatt cggttgccgg gcaggtcagc
    36481 catattccga caacgccgga actggcagag ctgaagcagg tgctgtgcta tgacggttat
    36541 ctcacgccac aaaatccggc gaatcaacat cattgtattg gtgccagtta tcatcgcggc
    36601 agcgaagata cggcgtacag tgaggacgat cagcagcaga atcgccagcg gttgattgat
    36661 tgtttcccgc aggcacagtg ggcaaaagag gttgatgtca gtgataaaga ggcgcgctgc
    36721 ggtgtgcgtt gtgccacccg cgatcatctg ccaatggtag gcaatgttcc cgattatgag
    36781 gcaacactcg tggaatatgc gtcgttggcg gagcagaaag atgaggcggt aagcgcgccg
    36841 gtttttgacg atctctttat gtttgcggct ttaggttctc gcggtttgtg ttctgccccg
    36901 ctgtgtgccg agattctggc ggcgcagatg agcgacgaac cgattccgat ggatgccagt
    36961 acactggcgg cgttaaaccc gaatcggtta tgggtgcgga aattgttgaa gggtaaagcg
    37021 gttaaggcgg ggtaatctgc tctcgcagtg tttgccggat gcggcgtgaa cgccttatcc
    37081 gacctacgtg tgacgtgtag gcctgataag acgcgataag cgtcgcatca ggcatggtgc
    37141 tccagacgcc gcaacttact gctgtgacgc ctgctgaaac aggttttccc acatatcggt
    37201 caccagcgcc tggtcacgcg gcgacaattc accggcacca atggcttttt ccagactctg
    37261 gctaacggtc gtatgcaccg cctgagcgga gtggtcatca ccactttcca gttctgcgat
    37321 ggctaatgtc aggtggccac gcaaataccc actggcaaac aactcatcat cactggcatg
    37381 gtcaaccata ccgtcgatta atgccagaat gcgtgattca aactccgcga tcatcttctt
    37441 tcctcattgt aaaacgtggc accggcttca gttgagcgct tccggccacg ggaactgttc
    37501 cgccgtaagt tccggcgtgt gataataatt ttgtagtgct ttaatgaagc gtgccgggcg
    37561 ttcaggaatg ccgtttgcta ggtaatccat cacctgcgca tgtacccgcc gttgaaacac
    37621 cacacgatcc ggttcgaagt cgccttccag attgtcgcaa ctgacgttaa acggatatcc
    37681 cgccgccaca cagaacaacc agtcgaacgc ctgcggcttc acttcaacat cttcaaactg
    37741 gctttgcgtt tgggcatcgc gtccgtccgg gcaataccag tagccgaaat caaccagctc
    37801 acggcgcgct ttcccagcaa tacaccagtg cgaaatctca tgaattgcgc tggcataaaa
    37861 gccatgagca aagacgattc ggttatacgg tacttccgca tcagcaggaa gatagatcgg
    37921 ttcgtcgtcg cctttaatca gacgggtatt aaattcatcg gcaaagcagc tattaaaaat
    37981 ttcaatcaac tgctcatagt ggtgtgtact gttcattagt tcatccccaa ccagtggagg
    38041 atctcctgtc cgtggctatc aaaaagtaat ttggcactca tcaccgctga gacaataaca
    38101 atcatcgggc ggatcagctt ttgtcctttg ctcaacacca gtcgtgaacc catgcgcgcg
    38161 ccgaggaact gccccaccag catgacaaag cccgtcgccc aaatcacttt gccaccgaga
    38221 ataaacagca gtaaaccgcc gatgtttgac gttgcgttga gtaatttggc gtgcgccgtg
    38281 gctttggcga ggttgaatcc gcacagcgta acaaaggcca gtgcgtaaaa cgatccggct
    38341 gccggaccaa agaatccgtc ataaaaaccg acgcagccac cggcaattaa cgcgaagggt
    38401 aaaccgtaca tccgacgctg gcgatcttct tcacccagct ttggcatcag caagaaatag
    38461 aggccgatac aaattaccag aatgggcaaa atctgccgta agacatcagc ctgaacgtat
    38521 tgcaccagca gtgcgccgct cattgagccg acaaaggtca tggcgatatt gagtttctga
    38581 tcgcttaaac taaccacttt gcggcgaata aagtagatag tagcggaaat agagccgccg
    38641 caggcttgca gtttattggt tgccagcgca ttagcgggag acatccccgc tgccatcaat
    38701 gccggaatgg tgagtaaccc accgccaccg gcaatcgagt cgataaatcc cgccagcatg
    38761 gcgacaaaaa agagaactcc cagcaacagc ggggaaacca taaacaggct attaaacgtt
    38821 tccattagat cacgtgctca tccagtagcg cctggcagga aggcggcaac ggaggcggtg
    38881 tcttcttctc aggctttgtt gttcccggtt ttggaggttc aaaccagctt tgcagttctg
    38941 ccccgcaacc atcgcctggt ggcggtaaag gttgatcttc acactccaga ctatcggcag
    39001 gacaacgtaa tcgtacatgc atatgcgcac gatgctggaa ccagggtcgc actttgcgca
    39061 accagtcgcg atcggtgccc gcatctaggc aaagttgttg tttaatcgcc ggattaacaa
    39121 aaatgcgcgt gacgtttttg tcctgggcgg cgagtttgat caagctgaaa atttctggct
    39181 tccacagcga ggagacaacg tgtttaccgt cgcgggaaac taagtccagt gcttgcgggc
    39241 gcaagagctg cgcggaggtc cagcgagttt tcggcagttg cagaaagata tcgacatcca
    39301 gtccggtctg gtggctggca tgaccgccgt tgaaacgccc accagcaggc atccccatat
    39361 cgccaatcag caccgtaccc atgcccagat tgctcacctg gctactcaga cgctggataa
    39421 acatcaccag atccgggtga ccgaaatagc gacgctgatc ggtacgcatg acctgataat
    39481 gttcggactg tatcggcagc gtgtcagcgc cgacaataca gccattagaa aaactgccta
    39541 tcgattgtgc gctacccggc acaggttggg ttattttttg ccacggcgtc gctgccaggc
    39601 tagcgctact ggcaagcaga gccagcagcg caatcgcggt tttgttcatt ttttaccagc
    39661 gtggaatatc agtcttcaca tcggcatttt gcgcccgctg ccgtaacagg tgatccatta
    39721 aaacgatcgc cagcatcgct tctgcgatcg gcactgcacg gatcccgaca cagggatcgt
    39781 gacggccttt ggtgatcatc tcaacttctt cgccaaagcg gttaatagtg cgccccggca
    39841 cggtaatgct ggaggtcggt ttcagcgcca tatgggcaat gatttgctgc ccgctgctga
    39901 taccgccgag aatgccgccc gcatggttgc tctggaaacc gtctttggtg atttcgtcgc
    39961 ggttctggct gccgcgcagc gccactacgt caaaaccgtc gccaatttcc acgcctttca
    40021 ccgcgttgat gctcatcagc gcatgggcga tgtcggcatc caggcggtca aagaccggct
    40081 cgccaagtcc ggcggggaca ccactggcaa caacggtgac tttcgcgccg atggagtcgc
    40141 cctctttttt cagcgcgcgc atcagttcat ctaacgcgtc gattttgtcc gggtccgggc
    40201 agaagaacgg attttgttca accagcgacc agtctttgat ttccagcgga atgtcgccca
    40261 tctgggtcaa gcagccgcgg atttcaatac caaatttctc ggcgagatat tttttggcaa
    40321 tcgcccctgc cgccacgcgc atggcggttt cgcgggcgga agaacgtcca ccgccgcgat
    40381 aatcgcgcag accgtatttt tgttcgtagg tgtaatcagc atggcctggg cggaaaacgt
    40441 ctttaattgc gctgtaatcc tgagagcgct ggtcagtgtt ttcgatcaac aagccaatgc
    40501 tggtgccagt agtaacgcct tcaaaaacac cggagagaat tttgacctga tccggctcgc
    40561 ggcgctgggt ggtatagcgc gatgtcccag ggcgacgacg gtcgaggtca tgttgcaggt
    40621 ccgcttccgt cagcggaatg cctggcggaa caccatcgac gatgcagccg agcgccagcc
    40681 catgcgattc gccgaaggtg gttacgcgaa agagttgtcc aattgtgttt ccagccatca
    40741 cggctccgtt attgttgtgt ttgcgtgttt acttaatctt tataaatcgc gaaatattca
    40801 cgggccgcca gcagctgctc tttggtgagc ataaacacac catcgccgcc gttatcaaac
    40861 tccagccagg tgaacggaac atccggatat tgttccataa gatgtaccat gctgttgccg
    40921 acttcacaaa tcagcacacc atcatcagca aggtaatctg ccgcgttacc gagaatgcga
    40981 cgcgtcagtt tcaggccgtc ggtgccagat gccaggccca gttccggctc gtgacggtat
    41041 tcgtttggca ggtcggacat atcttccgcg tcgacatacg gcgggttagt gacaatcaga
    41101 tcgtattgca ctttcggcaa atcacggaac agatcggaac gaatcggaat gacgttgtgg
    41161 atcagaccgt gttcttcgat gttctgttcg gcaaccgcca gcgcgtcggg agagatatct
    41221 accgcgtcga cttccgcttc cgggaaggca taagcacagg caatggcgat gcagccgcta
    41281 ccggtacaca tatctaaaat atgctgcggt tgcttgctga tgagtccggc aaatttattg
    41341 ttgatcagtt caccaatcgg tgagcgcggc accagcacgc gttcatcgac gtaaaattca
    41401 tgaccgcaga accaggcttt gttggtcagg taagccaccg gaatgcgctc gttgacgcgg
    41461 cggatcacgc gttcaacaat acggtgtttt tcgctggagg tcagacgcgc ggtgcgcata
    41521 tcttccggga tatccagcgg caggtagagc gatggtaaca ccagctgtac ggcttcatcc
    41581 cacgggttat cggtgccgtg accgtaccag atatttgccg cgctgaagcg gcttaccgac
    41641 cagcgcaaca tgtcctgaat ggtttgcagc tcatttactg cttcatcaac gaaaatttta
    41701 tccacgtatt cctccagggc atgatcgcaa taatttcggc ggctagtttg ccatgaagat
    41761 gacgataaat cagcattcac gcgcggtgag tgaggaaaaa tacgtttaaa acgatcgatt
    41821 gtgctacgag tcgggtaaac tgtaggaaaa ttagaaatag agacagataa atgaaaaaga
    41881 aaacaacact tagcgaggag gaccaggctc tgtttcgcca gttgatggcg gggactcgca
    41941 agattaagca ggacacgatt gtccaccgac cgcagcgtaa aaaaatcagc gaagtgccgg
    42001 tgaaacgctt gatccaggag caggctgatg ccagccatta tttctccgat gagtttcagc
    42061 cgttattaaa taccgaaggt ccggtgaaat atgttcgccc ggatgtcagc cattttgagg
    42121 cgaagaaact gcgccgcggc gattattcac cggaattgtt tttggattta cacggtctga
    42181 cgcagctgca ggccaagcag gaactgggcg cgttgattgc cgcctgtcgc cgcgaacatg
    42241 tgttttgtgc ctgcgtgatg catgggcacg ggaagcatat tttgaagcaa caaacaccgc
    42301 tgtggctggc acaacatccg catgtgatgg catttcacca ggcaccgaaa gagtacggcg
    42361 gtgatgcggc tttgttggtg ttgattgaag tggaagagtg gttgccgccg gagttgccct
    42421 gaggagttga gcgtgttgtg ccaggagcgc ggaatggcag gggcgaatga ccctgccagg
    42481 tgagaaaagg tgcgctaata catattcagt atgtaagtga atcggcaggt agaatctatg
    42541 ggctgtcgag atgtcgtgtc cacgcgtagc ttcatatcat gcagttgcct ggacgtgatc
    42601 ggttgcggct ctggcgtcac cgtcactttg gcattatcac agccggaagt cgagaaggtc
    42661 atctgctgtg cgacaaaacg gcagtaggct ccacgctcgt caaaattact gccatcacac
    42721 agtttgccgt tttccatcag gaaagtgggc agtgtttgct caataacacc acctgttgac
    42781 tgcaacctga cgactttgtt atgtacaaca taattttcat tctaggaaac atcatacggt
    42841 gccagggact ctgtcgggca gtcagagcta acttttgttg gtcctataaa aattccggcc
    42901 ccgaaacgct gatccatagt actggcgctt tcaacataag acgacacaat ccagggacaa
    42961 ccgtgtaaat ctccatcgta ataaacgcca agtactaatt cattacatga ccaggccgtt
    43021 actggagaga gagcatcata ctcttctaat ccggggcagg tagaatccgg taaggttgta
    43081 ttattgcgct ccatcctgag ttcagggccg ggaggactcc cggtccaact aactaaaggc
    43141 aaacttgctg ccgcagttga attagattca tacgtgccat attcaactga ttgaggggtc
    43201 aggactgaaa gatgaaaacg atactttgca acaacgctgg tcgtccccgc gggtacatag
    43261 agtgtggcga tctttgtcgc agcctgtatc ccagaagcgc acagagccag caccaataaa
    43321 catataacat gacgtatttt atgcatttta tatatactca aacgtagctg tcaggctggc
    43381 ggtgaaatca ccgatggtta catcacgacc gcttttcgcc tggatccagg cccggaaatt
    43441 aatatcattg ttgccgttgg taagcgcaaa agtcgccccg gtggtgttgt taatatccac
    43501 ccgcttgccg tcgagcgttt ccatccctat ccccacgccc tgcgcggtgg aggtgtcgtc
    43561 gactgccaga aaacccggct gaccgctgtc ttccgttccg gtgagggtga ctttgacgtt
    43621 gtatcccgcc ggccctttgc agtctttcag cttaaacaca accggcagat gcgcggactg
    43681 cccggcgacc atcaaatccc ggttgctgac ggtgggaaaa tgaatttccg ccagattggc
    43741 accatccacc accagcgtgc aggatttact cagcaggttg ccgtaaaaat gcaggttgtt
    43801 atcgactgcc cgtgcccccg tgctggcggt cagcagcagg cctgctaaca gcgcataacc
    43861 gggtgaataa atgtgtgctg ttttcattgg taatccaccc gtaaggttgc gtaagcagaa
    43921 aaattcgtgt ccgtcagcac cgcatcagcc gctttgaccg gtaccgcctc cagctttggc
    43981 ttctggctaa aatcggtggc gttaatggaa agcggctcgt tgagcttaaa acgctgaccg
    44041 tcatgctgaa gctcaattcc gaagcccggt acatccgtct caatagcaga gtcgttgtag
    44101 gaggtggcag tccccgtcca ggagagtgtc atattccact cgggatcgcg gacatccgag
    44161 tcgcagctca gttcgtagtc cactttctga cgaccataat tgccatcaat gctgtcgata
    44221 ataagatcgc gaaattgcac ttcgatggtt tgcccgccgc tgatgctaca ggcgggcggc
    44281 gacatcagcg taccgtggaa tgtgatgtca tcttccgccg ccagcacagc cccgcaacca
    44341 gcgcacagta aaaggagcag gaattttttc attggtaatc caccaccatg gtcatcgtgg
    44401 cgttaaactc cgacggcgtc agttgcaccc cgctctgttt taccggcacc gctttcagct
    44461 gaggctgagt gttgacatta aaaggcgtcc agttattttc accaactgca aacagggagt
    44521 tgtcggcggc gttttctatc cgcaccccca ggccggcgat atccgtgctg agaaccgttt
    44581 ctccgttgat cacggcggta ttgcctttga gctgcatccg taagtcatcg ctgactttat
    44641 taccgcagtt gagggtgtat ttcgcatcct gttggtaatt ggtaccatcg atcctgttga
    44701 ttatcacatt gccaaatttc acagcgcttc ccttcacgga gcaaggcggc ggtccatcga
    44761 ccaggattcg cagcgtcaga tccacctgtt tgatatcact gccagcaaca gccaaaccgc
    44821 tgatgcccca tccggccagc accagcaacc ctgcgcccgc tacaaacttg tgactcataa
    44881 gtccctcccg gcatcagcct ttgcggctct gctcttcatc cacctggcag ctgttgccgg
    44941 tgcatatgaa gtacagcggc atccgcgcgc cgtagtcgtt gatgtaagtc agcaccggca
    45001 cgctgtccat cttcgcattc agcggtgcgg aggttttcgg ctccatcatc agcggtgtga
    45061 atcccgccgg cgggttgccg ccttttttag cgctggcgtt gctgatgatc acgtagtacg
    45121 gcgtcgggtt attgacggtg aagtcctggc cgttacggat gagcgtcacc ttatgctgcc
    45181 acggactttt catgctggtg ttctccagcg ccttcggtcg ccagaacagt ttgatacgcg
    45241 tctgcagcgc aatctgcagt gtgttcggtt tatttgattt cggcggaatt tcgcgcacgt
    45301 tgaaatagaa cacgctctcg cggtcggccg gcagtttgtt gatatccggc agcccctgca
    45361 ccttcacctg gccgttcatc atcgcatcga tacgctgcac tggcggaagc accgcaagcg
    45421 gcgagctgat tttgttgccc tttgcatcct ctatccagct ctgcgccaga taaggcagtg
    45481 tgggatcgtt gttgcgcagc gtgacgctga tggacttgtc gctttcgtta aacaccagcc
    45541 gtgtcctgtc gggcgtcacc gaagcgtggc tggagaaact gaccactgtc gccagcagcg
    45601 ccagtgccgg gacaacgatt tttgcagtgt tagtcagatt aaacatggat cattccttaa
    45661 tcacgttact ggtttactga aattgaagtc ggaggttccg ttggcgtcac ccgctgtgtc
    45721 tgttcctgaa tcgccggttt gaccgccggc gaagtgatag gtgctacatt gcctttatgc
    45781 tggcatggca gtaacaagcc gttaaacaga tcggcaggca gcgggtccgg caggttgata
    45841 tcgcaatgcg caacaccact ccagaacaca ctcatgtgtt cgccaggttt caccccagcc
    45901 agataaacat tgccgtcatc atcgacaagg cccactgtct gctcgttatc attttttact
    45961 tctgcgccaa acggaggatg gctgccgtcg ctcaggcgca gcaccgccat cgctttttgt
    46021 ccgctaatga cagcaaattt acggtagcca atggcacctt cagttagcgt ggcttgcacc
    46081 accgactggg ttgcttcagc gttttcaggc aatctgttga ggtcgatata cgcctgattg
    46141 cggtaatagt tattgacgtc agaaacgacg gctttaccaa acatattggt ataaacagca
    46201 gccccgttac cttccaccgg aacatcggca acgccatcgg catcaatcaa caatcgtgtc
    46261 ccgcccatat tctgggtacg gtgaagtgcg ccaccgtggg cagtaagcgt tgcgccgccc
    46321 tgtaacgaca agcccgcaga ggtgtattgc ccttcatggt agttcgcact gaggtcaacc
    46381 tgcgccagcg aaccatcatg gctgtaatag ccgtcaacgc tggtgtgttt gtcactggtg
    46441 ccgacgttca actgatagtg agtcgcgtca tcgacacggc tgaaataacc gacctgactg
    46501 ctgtccgtcc cactgccata gttaccgtta tagctaacgg tactgttgtc gccccacggc
    46561 atactgagcg aaatgtacat gcctttgtcg gcctggttgt catactcgta acggtagcca
    46621 gtcatcgaga tgctgacatt acgaatactg cccatattga aatagtgcga gagcatgatg
    46681 ttgtagtttg tctgctcctc gcgatcccag taggtatggc gggtatagtt gagataaacc
    46741 gaaacaccca catcgcggaa gttctggtta taagtggcgg tgtacatctc tttgtcgttg
    46801 cccgtgcgta ccattccgct gtcactggca tccagatact cgctcatggt cataaagttc
    46861 tcttccgaga agcgatatcc agcaaaagta acgcggctgt tgagctggtc aaagtctttg
    46921 gaataactca cacggaagga gttaccgtcc agcgaacctt tgccataagc ggtgtcttta
    46981 tccagtttgg tgtgcgagtg agtaacatca aacgcgaccg cgccgaatgt agacaaatcg
    47041 cgaccgacgc caagcgccgc agactgatag ttttcatctc ccagtgcgcc gccatataac
    47101 gaccagccgt tagcgatccc ccaggaagct tcagcaccag aaaaaaattc accctcgata
    47161 tggtgtcccc actcttgcgg acggcccatc atgatcttat agcgaacctg acctgggcga
    47221 gtgaggtatg gcatcgaggc ggtgctgatg tcatattcct gcacctggcc gttctgttct
    47281 tcaatgcgaa tatgcaacgt accggagaca gaatcaccaa gatcctgaat acgaaacggt
    47341 ccagccggaa cctgcgtttc gtaaatcaca cgccccatct ggctgacggt cacttttgct
    47401 gtggtgtgtg ccacgccgga aatgtctggc gcgtagccgc gcagattggg aggcaacatt
    47461 tgatcgtcag tactgacact gccaccaaca tagttaaagc catcaaaaat atcggatctg
    47521 aggtaatcct cgcccagcgc cagtttggct tttaatgatg gtaacgcccg ccaggcatag
    47581 tagcgactcc actcccattt tttttgagtt tcatcgccgc tgaattcatc gtcatcatca
    47641 ttactgcgag tatgttgata gttagtctgc cagtcagcac gcatacgcca cggccccagg
    47701 ttaaccccga ccgtcccgtt gccgctgatc tcgttactat catcaccgcc attttcttcg
    47761 tgccgggttt gtgcgttgat gctgtagtcc gcgacgatcc cggagatgcc gtcatcccaa
    47821 cgtgaaggcg gatcccagtc gggataagta tattcgaggt aagcctgtgg cagtgaaatg
    47881 actaatgcgg actggcttaa atcagcctta atttccatgc cttccagttg accggatttc
    47941 aggcatttag catcgtggct ccattgcaga tttttcgcca cgtcttcttt taaaccaaac
    48001 tgcgctacca gttccggtgt cagacaagca tagcttttgc tcgcgtcatc ttcaccagca
    48061 taccagtaaa tatcgtactc ttccgccaat ggctgtttat ttagttgaac ctgtaaattg
    48121 tattttccgg gctcaacata tccctggctg gaaaaacgct tcagatcaat ttttgtgtcg
    48181 ccttttaatt ccagaaaacg ggaatcaaac tgaatgtcgt cttcagccca gacactgcta
    48241 taactccctg acattgccag cgcaatgcac cacggaagaa tccgtaatcg aaaaagagaa
    48301 tggtcaggca tacacgtttc tatccgtttt tattcttgtt cggtcaaaat ccatttaaat
    48361 cccccatagc agtgtcgcta tgggggtatg ccttacaggt aagtaatctg gaaggtggta
    48421 tttgcttcga aattacccag atctggcgca tcagctgcgc cgaccagcca ggctttaaag
    48481 ttcagtgtct gcttcgcttt gcctttattg gtagaagtgt ctttcactat tttctggtca
    48541 atacccatgc cgcttttgta agaggtaccc agagagtcac cgatcgccag gctaacattg
    48601 ttaaatgccg caccggtatc ggtattgaaa atggtgtaat aattgccgct atttgcagaa
    48661 gaaacggtac cagtaaaggt agtggtcatt gtttcctgag tatcaaatac gcaatcttgc
    48721 agacgaatct ggaaactttt cggcgtggtg gtgccgttat ttttcagttt ttcggcaccg
    48781 atatcaccca tatcaacttc cagtttgtga tcgtccggaa cgatagaaca cactgagtct
    48841 tcaatggtgc cgtaaaaacg tgcagtaccg ttgttacccg cagcgattgt tgccgtagag
    48901 gtgaaaacgc ccatcaccat tgccgcagca atagctgttt taa
//
LOCUS       DAZGLJ010000009        48682 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000009 DAZGLJ010000000
VERSION     DAZGLJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.009"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            48..1028
                     /gene="ydcK"
                     /locus_tag="ACHK49_000485"
     CDS             48..1028
                     /gene="ydcK"
                     /locus_tag="ACHK49_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO9261180.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDGVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(1023..1559)
                     /gene="rimL"
                     /locus_tag="ACHK49_000486"
     CDS             complement(1023..1559)
                     /gene="rimL"
                     /locus_tag="ACHK49_000486"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO9261181.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(1622..1846)
                     /gene="ydcH"
                     /locus_tag="ACHK49_000487"
     CDS             complement(1622..1846)
                     /gene="ydcH"
                     /locus_tag="ACHK49_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO9261182.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(1986..3641)
                     /gene="opgD"
                     /locus_tag="ACHK49_000488"
                     /gene_synonym="mdoD"
     CDS             complement(1986..3641)
                     /gene="opgD"
                     /locus_tag="ACHK49_000488"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO9261183.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATIFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(3866..5209)
                     /gene="ydcJ"
                     /locus_tag="ACHK49_000489"
     CDS             complement(3866..5209)
                     /gene="ydcJ"
                     /locus_tag="ACHK49_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO9261184.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            5426..6349
                     /gene="ydcI"
                     /locus_tag="ACHK49_000490"
     CDS             5426..6349
                     /gene="ydcI"
                     /locus_tag="ACHK49_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9261185.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(6387..8027)
                     /gene="trg"
                     /locus_tag="ACHK49_000491"
     CDS             complement(6387..8027)
                     /gene="trg"
                     /locus_tag="ACHK49_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO9261186.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            8426..8608
                     /locus_tag="ACHK49_000492"
     CDS             8426..8608
                     /locus_tag="ACHK49_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFO9261187.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSRLGQARGFSPRI"
     gene            complement(8647..8820)
                     /locus_tag="ACHK49_000493"
     CDS             complement(8647..8820)
                     /locus_tag="ACHK49_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9261188.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(9065..9595)
                     /gene="cybB"
                     /locus_tag="ACHK49_000494"
     CDS             complement(9065..9595)
                     /gene="cybB"
                     /locus_tag="ACHK49_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO9261189.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            9784..10785
                     /gene="gap"
                     /locus_tag="ACHK49_000495"
     CDS             9784..10785
                     /gene="gap"
                     /locus_tag="ACHK49_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO9261190.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFLWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(10827..12266)
                     /gene="aldA"
                     /locus_tag="ACHK49_000496"
     CDS             complement(10827..12266)
                     /gene="aldA"
                     /locus_tag="ACHK49_000496"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO9261191.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPAAEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVDGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(12463..13263)
                     /gene="ydcF"
                     /locus_tag="ACHK49_000497"
     CDS             complement(12463..13263)
                     /gene="ydcF"
                     /locus_tag="ACHK49_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO9261192.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(13535..17437)
                     /gene="hrpA"
                     /locus_tag="ACHK49_000498"
     CDS             complement(13535..17437)
                     /gene="hrpA"
                     /locus_tag="ACHK49_000498"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO9261193.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRIDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            17638..18243
                     /gene="azoR"
                     /locus_tag="ACHK49_000499"
     CDS             17638..18243
                     /gene="azoR"
                     /locus_tag="ACHK49_000499"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO9261194.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAVPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(18294..19610)
                     /gene="ynbD"
                     /locus_tag="ACHK49_000500"
     CDS             complement(18294..19610)
                     /gene="ynbD"
                     /locus_tag="ACHK49_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO9261195.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(19600..21357)
                     /gene="ynbC"
                     /locus_tag="ACHK49_000501"
     CDS             complement(19600..21357)
                     /gene="ynbC"
                     /locus_tag="ACHK49_000501"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO9261196.1"
                     /translation="MENSRIHGEHFFTSSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTVFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(21373..22269)
                     /gene="ynbB"
                     /locus_tag="ACHK49_000502"
     CDS             complement(21373..22269)
                     /gene="ynbB"
                     /locus_tag="ACHK49_000502"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO9261197.1"
                     /translation="MLEKSLATLFALLILATLINHFLLWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(22269..22874)
                     /gene="ynbA"
                     /locus_tag="ACHK49_000503"
     CDS             complement(22269..22874)
                     /gene="ynbA"
                     /locus_tag="ACHK49_000503"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO9261198.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(23045..25351)
                     /gene="ydbD"
                     /locus_tag="ACHK49_000504"
     CDS             complement(23045..25351)
                     /gene="ydbD"
                     /locus_tag="ACHK49_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO9261199.1"
                     /translation="MFKSRICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNNTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(25415..26275)
                     /gene="pdxI"
                     /locus_tag="ACHK49_000505"
     CDS             complement(25415..26275)
                     /gene="pdxI"
                     /locus_tag="ACHK49_000505"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO9261200.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARNIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(26506..27096)
                     /gene="paaY"
                     /locus_tag="ACHK49_000506"
     CDS             complement(26506..27096)
                     /gene="paaY"
                     /locus_tag="ACHK49_000506"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFO9261201.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            complement(27078..28028)
                     /gene="paaX"
                     /locus_tag="ACHK49_000507"
     CDS             complement(27078..28028)
                     /gene="paaX"
                     /locus_tag="ACHK49_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFO9261202.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(28129..29442)
                     /gene="paaK"
                     /locus_tag="ACHK49_000508"
     CDS             complement(28129..29442)
                     /gene="paaK"
                     /locus_tag="ACHK49_000508"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFO9261203.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGGAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(29469..30674)
                     /gene="paaJ"
                     /locus_tag="ACHK49_000509"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(29469..30674)
                     /gene="paaJ"
                     /locus_tag="ACHK49_000509"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFO9261204.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPHSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(30674..31096)
                     /gene="paaI"
                     /locus_tag="ACHK49_000510"
     CDS             complement(30674..31096)
                     /gene="paaI"
                     /locus_tag="ACHK49_000510"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFO9261205.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(31086..32513)
                     /gene="paaH"
                     /locus_tag="ACHK49_000511"
     CDS             complement(31086..32513)
                     /gene="paaH"
                     /locus_tag="ACHK49_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFO9261206.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(32515..33303)
                     /gene="paaG"
                     /locus_tag="ACHK49_000512"
     CDS             complement(32515..33303)
                     /gene="paaG"
                     /locus_tag="ACHK49_000512"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFO9261207.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(33303..34070)
                     /gene="paaF"
                     /locus_tag="ACHK49_000513"
     CDS             complement(33303..34070)
                     /gene="paaF"
                     /locus_tag="ACHK49_000513"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFO9261208.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATD
                     TSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(34067..35137)
                     /gene="paaE"
                     /locus_tag="ACHK49_000514"
     CDS             complement(34067..35137)
                     /gene="paaE"
                     /locus_tag="ACHK49_000514"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFO9261209.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGERLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(35145..35642)
                     /gene="paaD"
                     /locus_tag="ACHK49_000515"
     CDS             complement(35145..35642)
                     /gene="paaD"
                     /locus_tag="ACHK49_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFO9261210.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(35657..36403)
                     /gene="paaC"
                     /locus_tag="ACHK49_000516"
     CDS             complement(35657..36403)
                     /gene="paaC"
                     /locus_tag="ACHK49_000516"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFO9261211.1"
                     /translation="MNQLTAYTLRLGDNYLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGIDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(36412..36699)
                     /gene="paaB"
                     /locus_tag="ACHK49_000517"
     CDS             complement(36412..36699)
                     /gene="paaB"
                     /locus_tag="ACHK49_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFO9261212.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(36711..37640)
                     /gene="paaA"
                     /locus_tag="ACHK49_000518"
     CDS             complement(36711..37640)
                     /gene="paaA"
                     /locus_tag="ACHK49_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFO9261213.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            37925..39970
                     /gene="paaZ"
                     /locus_tag="ACHK49_000519"
     CDS             37925..39970
                     /gene="paaZ"
                     /locus_tag="ACHK49_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFO9261214.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            40218..42491
                     /gene="tynA"
                     /locus_tag="ACHK49_000520"
     CDS             40218..42491
                     /gene="tynA"
                     /locus_tag="ACHK49_000520"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFO9261215.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDETL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(42549..44048)
                     /gene="feaB"
                     /locus_tag="ACHK49_000521"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(42549..44048)
                     /gene="feaB"
                     /locus_tag="ACHK49_000521"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HFO9261216.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            44284..45189
                     /gene="feaR"
                     /locus_tag="ACHK49_000522"
     CDS             44284..45189
                     /gene="feaR"
                     /locus_tag="ACHK49_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFO9261217.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGHLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(45361..45690)
                     /gene="ydbL"
                     /locus_tag="ACHK49_000523"
     CDS             complement(45361..45690)
                     /gene="ydbL"
                     /locus_tag="ACHK49_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO9261218.1"
                     /translation="MMKKTLLFCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(45695..45880)
                     /gene="ynbE"
                     /locus_tag="ACHK49_000524"
     CDS             complement(45695..45880)
                     /gene="ynbE"
                     /locus_tag="ACHK49_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO9261219.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(45877..48516)
                     /gene="ydbH"
                     /locus_tag="ACHK49_000525"
     CDS             complement(45877..48516)
                     /gene="ydbH"
                     /locus_tag="ACHK49_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO9261220.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNSEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
BASE COUNT        12241 a        12875 c        11537 g        12029 t
ORIGIN      
        1 ccgcagaatt tgctacggtt atagtaagtc acctgtggga aaacgctatg cgcaaatatc
       61 gtctaagtga agaacagcga gcctttagtt atcaggaaga tggcactaaa aaaaatgtgt
      121 tattacggca gattatcgcc attagcgatt ttaacgatgt gatagcagga accgctggcg
      181 gctggatcga tcgcgaaacg gtgttagcac aggaaggaaa ttgctggatt tacgaccaga
      241 atgctattgc gttcggcgga acggtgattt ccggtaatac gcgcatcacc ggcaccagcg
      301 tactgtgggg agaggtttac gcaacggata atgtctggat cgataatagt gaaatcagcc
      361 agggtgcata tataagtgac ggtgtcacca ttcatgactc actggtttgc gggcaatgta
      421 gaatatttgg tcacgcctta attaatcaac attctatgat cgtcgcagca caaggcttaa
      481 cgcctgacca tcaactcctg ctacaaattt atgatcgcgc aagggttagc gcctcgcgta
      541 ttgtccacca ggcacaaatt tatggcgatg ccgtcatccg gtatgctttt attgaacatc
      601 gtgccgaagt ttttgatttt gccagtattg aaggtaatga agagaacaac gtctggctat
      661 gcgattgcgc taaagtctac ggtcacgcgc aggtgaaagc tggcatagaa gaagatgcca
      721 ttccaacgat tcattacagt tcgcaggtgg cggaatatgc cattgtggaa ggtaattgtg
      781 tgttaaaaca tcatgtcttg attggcggaa atgccgtggt acgtggtgga ccgattctgc
      841 tcgacgagca cgttgtaata caaggtgaga gccgtattac aggtgcggtg ataattgaaa
      901 accatgtgga gttaaccgac cacgccgtcg ttgaagcctt tgatggcgac acggttcatg
      961 tccgtggtcc aaaagtcatt aacggcgagg agcgcattac gcgaacgccg ctggcagggt
     1021 tattatgaat cgataatacg cgcgtataag ttcacatcat cataggcatc attcaggaac
     1081 tcagcctgtt tcaggcaacc ttcaaggata aaaccattgc gcaaagcgac ctggttgctt
     1141 tgcggattgt ccacccgaca tttgatcacg aagcgtctaa gttcaccaga ctgggcgtaa
     1201 tgatgaatca atgcctgcag cgcctgagaa atgatcccct gcccctgatg agattcgtcc
     1261 agccagtagc ctatttcagc ggttttattc agtggttcaa tacgattaaa cgagataacg
     1321 ccgataagtt catcttcttt gaaaatcatg aacattttgg catagccgcg ttgatgcaac
     1381 atcacattac cctgcaccgt ttttcgcgtg tcctcttcac tctgaacgaa ctgcggccag
     1441 tttagcgact gctgtaacca ggttttattt ttacagatta actgataaag aggagtgacg
     1501 tgattttctg caacagcatg taattcaagt gattcgctta cttttatcgt ttcagtcatc
     1561 ttatctccag tcaatccatg cagttgttaa aaaagccgcc tggctggcgg cttaggaaag
     1621 tttacacctc tttgacgctc tcctgctgca ggattttgag catctcatct tttaactgta
     1681 gcttttgttt tttcatgcgg accacttccg cattgtaccc tcgaccgtcg gaaccttcct
     1741 ttctggcaat ttcatgatca agtttattgt gtttatcgaa caaggacata aagcgaggat
     1801 tttcgttttt cagacgggat attaaatctc gatattctgg aaacatacgc actccctgtt
     1861 agtggctgac aaggtgtatc tgaaatacac accatcagat tactcattta acttagggaa
     1921 aaatgcgagc gggatcgtgt ttaaccagac ggcacctccc gaagaaggtg ccgaagaaaa
     1981 aacgattaac tcatcacgcg gtcgtcaaca tactgacgtt tatccggcgc tggcgggaaa
     2041 tactgataca gccatgtttc actgatagcg tcgccctgac aacgcagata catccgcata
     2101 tcgaccggat cagtggagtc cgaagtcgga taccagtcaa actgaatacg ataaccatca
     2161 atgggttcaa tatagagaat ttcgatttgc ttcgcttccc cactggaaag cgtaatcacc
     2221 ggctcaatgc cttttggtgc ggcagctttc agatcaccac caacgaaatc gacggcaaaa
     2281 cgacgcgccc atttttcggg atagtgttca cctggtgccc aaccttccgg gaaaccgccc
     2341 atgccggtac gcgtcgccat aacgcgcgct aatgggcaat gaacaggcgg ttgcgcactc
     2401 cagtacagac gatactggaa tgcaaactca tcacccgcct ttacagcttt ttctggctgc
     2461 cagaagcaga caatgttatc cagcgtttcg cccgttgttg ggatttccat caggccgatg
     2521 gtgcccttac cccacttgtt acgcggttcc acccacagac ttgggcgttt gttataccag
     2581 cccataatgt cctgataatg ggagaaatca cgatccagtt gcaataaacc aaaccctttc
     2641 gggttgttgt cggtgtaagc attgaactgc aatttttgcg gattattcag cggacggcaa
     2701 atccactcgc cgttgccccg ccacatggac agacgatcag agtcatgaat ttgcggatga
     2761 atggtgtcgc acatccgacg ttcattagta ccgcagctga acatactggt catcggcgca
     2821 atgcccagct gtttaatgtc tttgcgcgca tacaggtgat tttccacatc cataatcacc
     2881 tgacttttct cacaatggat agtgaactta taggcaccag taatgctggc gctatcgagc
     2941 aacgcataaa cggtaaagat agttgccccc ggttttaccg tatcaaacca gaaggcggta
     3001 aagtcgggga actcttcttt actgtcggtg taagtgtcaa tcgccaggcc gcgggccgac
     3061 aaaccgtatt gatatgtgtc atcaacggcg cggaaataac tcgctccgag gaatgatact
     3121 acatcacggc gcgccagttc gggggcttta aacacgcgaa aaccggcaaa gccgagatcg
     3181 ctttgccctt ctaattgttt ggtatcaaca cctgcatcgt tgtatttgaa caactccggg
     3241 cgaaagtgaa tttcacgcgc cagatgtgtt gctggatcta cagaaaacat acgaacgcgg
     3301 cgacggaatc ccattcccat atggaagaac tgagcgtcca gttgacggtt ctcaacgtta
     3361 tgccagagcg atttttcggc gtcgtattga atactgttat aagcctgcgg cgtcattgtc
     3421 gccagcgtgt caggtaacgg acgaggcgca ccacgccacg ctgtttgcgc taagtcgtgc
     3481 gccattgact gtagaatgga gaagtcaaaa cgctgggttt gcccgtcggc aatatcagaa
     3541 tctgccgcga atgccgcctg agaaaaaaga gaagcaatgc cgctggtacc gcacacggcg
     3601 gccatagcca ttgaaccttt aataaatcgt ctacgatcca tacctgaaag tgagtccttc
     3661 tggtgttgtg aatggtcccg cgctggtcgt tttcagcggc aagaaagtgc ttatttatga
     3721 ccgctcactc tagacaatat ttattaataa tccaattgtt gaactcagag aatatgcata
     3781 aatcggcaat ttttttatgt cgatgggatg agcctgattc gtttgtaaaa tcaggcaaat
     3841 ttttgcaaag gcggatggtc agaatttaaa gcaaaccaca gcgacgttta ctgcgttctt
     3901 ccgcttcctg ataaagctgg aactcatcca aaaccggaca acccaacgcc tgctcaaatg
     3961 cctcgcgact ggcattaccg tgattgcgtg cctgcgtttc attacccaga tttgactgga
     4021 agatccccgc cgcgctaacg ggcaagaaat cttcataggt aatgggttgc gccgcgaccc
     4081 aaccacgttc aattaagggc tgtggatcgt ctccgggatg aatcgcctga cgatgcgcct
     4141 cacctgaagg cgtcagacgg taccggaacc atgccagccc ttgctgacgc attaaaaact
     4201 cactgtcagg aaaagtgcgg aaggtttcct gtaaatgcat ttggtgagtg agattatcct
     4261 gcccggttcc agcgttacgc agaagatcat catacagttg tcgccctttc ggcgttaatg
     4321 ccacgccacg ctgctcaatt tcaccaaagc gcgcggtatg cgtgccctgt ttctgccccg
     4381 caaacaacac cgtctcttcc agtgctttaa agctggtctg gcgtagtaaa atcggtacct
     4441 cgcggcgcgg cggcccctcg atgagaattt tgggttcaat tccgcattca ggcatcatcg
     4501 actgcacccg gtcaatatcc agcgtacgtg gcgtcaggtg gttgatatgg catccaggaa
     4561 aacagaccac atcagcaatc aaccgatgtt cgttgtgcaa ggcgcgatag gtttcttcat
     4621 ctaccgttgc tgactggtgc cagcgaaacg tttccagggc ttcctgcaca aactcctgtg
     4681 cctgtgtttc gttaaaaccg ccctgctgct catattcctc taacagttgt cgacaacgtg
     4741 gggtgaagat atcgcgctga cgcagaatct ccgccgcttt ctggcgcaaa atttcgttct
     4801 cgataagctc aaggcggagt aaggaggtaa aaacgcggaa gggattacgc gccagagaag
     4861 catcatcaat gggccgaaat gctgtcgaat gtaccggcac ccctgcctgc gagagatcgt
     4921 aatagctcac cgggtacatc cccataatgg caaacatccg ccgaagagta gcaagctctt
     4981 gtgcagtccc aacgcgaatc gccccatgac gttcaacatt cagtcgcgcc agctcgtctg
     5041 catttaccat tttttcgtgc agttgaggat tgttttccag cacagccaga ttcacatcag
     5101 ctaccagttc cagcagcgtg ccatattgcg gaacttcttg ctggtacatg gctgacattg
     5161 cctgcgaaaa ctgttcccga atctcatccg ccgtgatgct gttcgccata gtgtctgcct
     5221 ccagtggtca gtaatctgga gtgtaggtaa ccgcattcac tctggcggga agaatttaca
     5281 aactgtgatc tcgccgcgaa aacatcaata ttatccattt tgctgtaaca caattgcttt
     5341 aattgttaat aatattttgc aatcaagtta tcataatcaa acaacttcac ttgtcagcga
     5401 caccgcttcg tttttaacat cgcttatgga aaaaaatagt ctgtttagtc agcgcatccg
     5461 tttgcgccac cttcatacat tcgtagctgt cgcacaacaa ggaactttgg ggcgcgcggc
     5521 tgaaaccctt aatttgagtc aacctgcgct ctctaagaca ttgaatgaac tggagcagct
     5581 gactggcgct cgcttgtttg agcgtggtcg tcagggggcg caacttacct tacccggcga
     5641 acaattttta acgcatgcag tcagagttct tgacgccatc aacactgccg gacagtcgct
     5701 tcatcgtaaa gaaggtctta ataatgatgt cgtcagggtt ggtgcactac ctactgcggc
     5761 actggggata ttaccttcgg ttataggtca gtttcatcag caacaaaaag agacgacctt
     5821 gcaagttgcg acaatgagta accctatgat tctggcgggt ttaaaaaccg gggaaatcga
     5881 tatcggcatt ggtcggatgt cagatcctga actgatgacc gggcttaatt acgaactgct
     5941 gtttcttgaa tcgttgaagc tggttgtccg ccctaatcac ccgctacttc aggagaacgt
     6001 aacgctaagc cgggtgctgg aatggccggt cgttgtatca ccagaaggca ctgcgccacg
     6061 ccagcattca gatgcattag tgcagagcca gggctgtaaa attccttcgg gttgtatcga
     6121 aacgctgtct gcttcgctat ctcgtcaact tacggttgaa tacgattacg tgtggtttgt
     6181 cccttctggc gctgtaaaag acgacctgcg tcatgccacg ctggtggccc tgcctgttcc
     6241 gggacatggt gcaggcgaac cgattggaat actgacccgc gtagatgcga cgttctcttc
     6301 tggttgccag ttgatgatta acgctattcg aaaatcaatg ccgttctgaa aggtgaaggg
     6361 atctgtcgat ccctccttga acattttcac accgtagcga aactaactgg ttcacccgct
     6421 ccgcgaggtt ctgccgacac agaatgtttg tgcagacgga atacatccac cgcctcagtt
     6481 aatcgtgccg cctgttcttc aagggacacc gccgccgctg aggcctcttc taccagagaa
     6541 gcattctgtt gcgtcacctt atccatttca gaaatcgcct ggctaacctg cgttatgcct
     6601 ctactttgtt catccgaggc ggcggcgatt tcctgcatga tatgtgtgac actcgcgacg
     6661 gcatcaacaa tagtgctcat ggttttccct gccgttgcca cctcatccga ccccaggtca
     6721 attaacctga ctgattcact gatcaagcct tcaatctctt tcgccgcttg ggcgctgcgg
     6781 cttgcgagtg tccgtacttc gctggcgaca acggcaaatc cacggccttg ctcacccgct
     6841 cgcgcggctt caacggcagc attcagtgcc agaatattcg tctggaaagc aatactgttg
     6901 atgacggcgg tgatctcgga aattttcttc gaacttgtag agatagcgcc catcgttttt
     6961 actacaccgg aaaccgtctg cccgccatcg ctggctttaa tagaagcctc ttgcgccagt
     7021 ttgctggcat gatgcgcgtt atccgcattc tgtttcaccg tcgcagtgag ttgctccatg
     7081 ctagcggcag tttgttcgat agccgccgct tgttcttcgg tgcgagatga caggtccgca
     7141 ttgccagctg aaatttcgct ggtgccacga taaatctctt ccgcaccctg tcgaacagtc
     7201 cctactgtca tccccagtga atgctgcatt tgctgtaaat gacgacttaa gcgaccgatt
     7261 tcattacgac ccgccggttc atcattcatc gtcagatcgc cactggcgat tttttcaatc
     7321 cgttgtgcgg catgttgcag tggacgaatg acgatccgac gtagcaccat aaatgttatc
     7381 agcgtcatga ccagggcaag cacaaacgcg ccaatcatga acatcccacc caggcgggtg
     7441 cgctgatggg ccagttccgc cagttggttg gctctggtgc tacgtatctt aacggctttg
     7501 ttcaaaatat cggtataagc attatccagc ggtcggatct gctcactttc atgattgata
     7561 atcgcttcaa acatgccatt tttggcatat ttcaacatag gttgcatacc cgtgatataa
     7621 gcctgaaagc gttgatttaa ttcagtgtcg agggcttcat cagcaggtgt tttcaccggt
     7681 cgattctgat aagcacgata accttgctgc gactgtttaa tctccgattc ggcttgcgca
     7741 atatttcgct tcattgcttc catttccgca atacgacttg cagccccggc ctgaatcata
     7801 ttgatccggg ctgaacgcaa aaaatctgaa ctgttcgcta acccggtccg aatctcaatc
     7861 tccgcagtaa catcacgctg atcgcgatcg gcctgccaga ggaaatagcc agccagagca
     7921 gaacttaatg cgaatagaac taagataccg ccaagaatgc aggcaaataa cggaaccagc
     7981 ctgatgtgat gcaaaaaacc taatcgatgt gagggagttg tattcattac cgttgtctct
     8041 cgtccaggtt tactcctgat agaaagtcat cggcacttga cggtaattac ttatgaccaa
     8101 aaacctcttt gcgtcacatt tttacaacaa ttattgcaga gaaaattaat gctattggaa
     8161 tgaagagagt aacgaatggc taaactggct aacccgaatg acgaaaaatt ctggaaaaca
     8221 gcacgcaatt tcgtgaagtg attattttcg tccctaagga aacctcatag gatgcctgcg
     8281 ggtgcctgag actttctgtt ttcaggctaa ccgggaatca gaaagacgaa ggccccgacc
     8341 gatatttcta tcaaccgagg ccaacgaatg aacctagcaa ttcagatctt agcctcttac
     8401 ccgccatccg gcaaggagaa aggctatgaa gcacaaccct ctggtggtgt gtctgctcat
     8461 tatctgcatt acgattctga cattcacact cctgacccga caaacgctct acgaactgcg
     8521 gttccgggac ggtgataagg aggttgctgc gctcatggcc tgcacgtcca ggttagggca
     8581 agcgcgggga ttttccccgc gcatctgact agcagattca ttttctcatg gcaccccttt
     8641 tacacttcag ttagtgcatg tttttttcga ggcactaata gaaccatcat tacagacaaa
     8701 tttgccgcct gccgtacagt gtgagatccc ccctttcgaa ccagaacagg gtttgcgccc
     8761 ggcatcggca taaaaggaaa caagcgttcc cataaacaaa ataagtgcca gttttttcat
     8821 ataacctcca tggtttatcg aatcttatgt tcgatttgaa cgttatacgc tggtttattt
     8881 tgataccgtt gatgagatca aagatcgggt gaattgatta aaaataagcc agatggacgt
     8941 atgggcaagg attatggttt tatttgtcat acaaataaat ataataggcg cttccgatgt
     9001 agacccgtat tcttcgcctg taccacgggt cggttttagt acaggcgttt tctttaaata
     9061 tccttcagga acgtttacgc ggcatcatgc gtagaagtgt gttgtccttc cagaaatagt
     9121 ggtgtgccag tgcagccgca gcgtgcaacc cgatgacaaa atatcccaga ttcgccagcg
     9181 tctcatgcca cgactttaag ctatcaaccc gttcgaaatt ggcctctgaa gcgtaaggca
     9241 tcgtcaaacc aaacgcaaac cacgggttgc cccggttata catcatcacc aaaccaatca
     9301 ctggcagcgc aataaacagg agataaatca ccaaatgtcc caaatgcgcc agtcccgtca
     9361 tcatcggttt tggcttaggt ataatcggcg gggttgggta tttcagtctt aacaaaagac
     9421 gaaccaccat cagcacgaga attgagatac cacaggaaac atgaaccatg ttgatgagtg
     9481 gccgatcgct acgtgggaag aaaccacgaa actccattgc gcaatacgct gcgataacca
     9541 gcaaaaagac cagccagtga atgctgattt gtaaccttga gtatttattt tccataacat
     9601 ttcctgcttt aacataattt tccgttaaca taacgggctt ttctcaaaat ttcattaaat
     9661 attgttcacc cgttttcagt acgttacgtt tcactgtaag aatggagatt gcccatccca
     9721 caatcctggt ctaagcctgg aaaggatcaa ttttcatccg aatgttcctg acaggagaaa
     9781 accatgagta aagttggtat taacggtttt ggtcgtatcg gtcgactggt gttgcgtcga
     9841 ttacttgaag tcaaaagcaa catagacgtt gtcgctatta atgatctcac ttccccaaaa
     9901 attctcgcct acctgctgaa acatgattca aactacggac cattcctctg gagcgttgat
     9961 tttacggaag attcacttat cgttgatggg aaaagtatcg cggtttacgc cgaaaaagag
    10021 gctaaaaata ttccgtggaa agcgaaaggt gcagaaatca ttgtcgaatg tactggcttt
    10081 tatacctccg ccgagaaatc gcaggcgcat cttgatgctg gtgcgaagaa ggtgttgatt
    10141 tccgcccctg ccggtgaaat gaaaactatc gtttataacg tcaatgacga cactctggat
    10201 ggcaacgaca ccattgtttc cgtggcgtca tgcaccacta actgtcttgc gccgatggcc
    10261 aaagccttgc atgacagttt cgggatagaa gtcggcacga tgacgaccat tcatgcctat
    10321 actggcaccc agtcactggt ggatggccca cgtggtaaag atttacgtgc ttcacgcgca
    10381 gcggcagaaa atatcattcc ccacactacg ggggcggcaa aagccattgg tctggtgatc
    10441 ccggaactga gcggcaaact gaaaggtcat gcgcaacgcg tgccggtgaa aacaggttcg
    10501 gtcactgagc tggtgtccat tctcggaaaa aaagtgactg ccgaagaggt gaataacgca
    10561 cttaaacaag caaccaccaa taacgagtca tttggttata ccgatgaaga aatagtctct
    10621 tccgatatca ttggcagcca tttcggttcg gtgtttgatg ccacgcaaac ggaaattacc
    10681 gccgtgggcg atttacaact ggtgaaaacg gtcgcctggt acgataacga atatggcttc
    10741 gtcacgcagc ttattcgcac cctcgaaaaa ttcgctaaac tctgacgcgc acaggcggag
    10801 gaaaaaacct ccgcctcttt tactcattac gactgtaaat aaaccacctg ggtctgcaga
    10861 tattcatgca ggccatgttt accatctgcg ccgccaatac cggatttacg ccatccggcg
    10921 tggaagcctt gcatagcttc gaagttttca cggttgatgt aagtttcacc aaacttcagc
    10981 cctttaatgg ctttcatcgc gacgttcaga ttttgggtat agattgatga ggtcaggccg
    11041 taatcactgt cattagccat tgagatagca tcttccagcg tgtcaaatgc gaccaccggc
    11101 agaaccgggc caaaggtttc ctcatgcata atcgacattt cctggcgaac atccagcagc
    11161 aatgtcggcg gataataata tcctttcccg tctactgctt tgccacccaa cgccactctc
    11221 gccccttctt ctactgcgcg cgccactttt tgctcgaccc tttccagcgc cgcggcgtta
    11281 atcaacggcc ccatcgcaat gtcgttgcgt tcagcggggt taccaaattg aaccgcctgc
    11341 atcgcttcac ccagccgatt gacgaactga tcataaatgc ctttctgtac ataaacacgt
    11401 tctgcacagt tacacacttg cccactatta atgacgcgtg aatcaacgat ggctttgact
    11461 gccagttcaa gatcggcatc gtccattacg atagctggtg ctttaccacc caattccagg
    11521 cacactttgg tgatgttttt cgccgcagtc gccataatct tctcacctgc agagacgctg
    11581 cctgtcatac tgaccattgc gacctttggg ttacccgcca gttcttgccc aacggtttca
    11641 ccacgcccca gtacaaggtt aaacacgccg cgcggaaggc ctatttcatc gacgattttg
    11701 gcgaatgcaa tcgcattgtt tggcgtaaat tcactgggtt taatgacgat ggtattaccg
    11761 gtcaaaagag cgggagccat tttgcgggca atgaggaaga acgggaagtt ccacggcaga
    11821 atgccggtag tcacaccaag cgcacgttta aacaaaagaa tattttctcc tggacgatcg
    11881 ctttgaataa tctcgccctc gtaacgccgt gcccactccg ccatgtaatc gatatagtcg
    11941 gcagtaaaag ccacttcgac ttcagccagc tgctggatct tgcccccttc ttcaacaatc
    12001 agcgcactga tttcactggc gcgttcgcgg atgccggcgg agattttgcg caaccaactg
    12061 gcgcgttcaa tagcaggcaa cgcttcccat tctggttgtg cacgttctgc tgcatcgatt
    12121 gccttacggg catcctcggc ctgaccatcg ggtatgcggg aaatgacagc ctctgcagca
    12181 gggtttacca catcaatcca tgcgtctcca cgccaggtaa caaactgtcc atcgatatac
    12241 ataggatgtt gaacgggtac tgacatgggc gactcctgtg atttatatgt ttgttttcgg
    12301 tgaatgcatt gttaattaac atcaatcatc agagagacgg catcgtgaaa ggacaattct
    12361 gtgaagggct tcataaaaac aagtgtaaaa ggaataaaag gcagaaataa aagtcaggag
    12421 tcatgcccgt ttgcaaacaa gtggttcaca aacgggcaat ttttaacgta acgagcgact
    12481 ttccatcgcc tcgatgagca ccgcatcatg tttcagcgtt tgccatgcat ggatgacttc
    12541 tgccggaaaa tcaacgtgaa cgataaaatc tcgcccgcgg ggaccgtagc catcgctatc
    12601 atcgcgtaaa cgcggcagct cgccagtgag tagtgagaga taacgctcaa ccggccataa
    12661 tccttgtaac tgattaataa agattacact gtctgcgtta tttcctaact gaggaacgaa
    12721 tccgggataa cttaaccagc gtggtgcatc gggattgtcc ccagtcatac ggcggaacgt
    12781 cgccatcgtg cgccgctgca tggtggggtc ctgaacaacg atagccgtat gaactcgttc
    12841 tacggcctga ttcaatagcg cgatgctaaa gcgtgcgttt tcaccgcagt ttgttgactg
    12901 gtcttcaatc cagatttttt catgcggaat gtgccagaac tgatgagcga tatccgccag
    12961 gatggtcgct tctgctctgc cagtggtgcg gatagtgttg tagtgcggat gctgtgcgat
    13021 ggcgctatac aaaaaagttg tcgagtgacc gataccacca ctaatcagta aaggaatttg
    13081 ctgatcgcga gcaatcttac atgccgcgtc gatagtcggc ataaccgcat tgcctgcaag
    13141 gatcacgcaa tcggcctgat acggcacctc accggagaaa tcatcctgcg ccagccactg
    13201 tccgataaca tttatggcat ctatagttgc cggcgaaagc gtcggaaacg gggtaatgtt
    13261 catggttcct ccttatcgtt cccttaagaa tactgcgtga gataatagtg agaagcggag
    13321 cactctgaaa ggaggaagtc gtatgtcctt aaccgccact ccgcaagcct gacatatctt
    13381 ttaaggaaat gctgtagata aacattattc atcttgcagc agagaattgt atttttctga
    13441 attcctgaat ttttaccctt accgttttag aattaataaa aaacccggcg ctaaatgctt
    13501 accgggtttt ttctttatca ggcaaatagc agggttaacc gctaatctgc tccatcgcct
    13561 gcaaaatacg cttatctgaa atcggataag gcgtaccaag ttgttgagcg aagtaactaa
    13621 cgcgcaactc ttctatcatc caacggatct ctttcacgtc ttcatcctca cgacgtgcgg
    13681 gcggcagttt gttgatccat tgctgccacg cctgctggac gttttcgact ttcagcatct
    13741 gagcacggtc gcgatgtgga tcaaccgcca gtttttccag ccgtttttca atcgcctgca
    13801 aatatcgcag cgtgtcgccc agccgtttga agccgttacc agtgacaaaa ccgcgatata
    13861 ccaacccgcc catctgcgct ttaatgtcag aaagccccag tgccatggtc atatccaccc
    13921 gccctttcag acgtttgttg atattgaaca ccgcagtgag gatttgctcg acctgcttcg
    13981 caatatccac caccgtgtca ttcagttcgg cgcgcacttt ttcatgcagc gcagcaaagc
    14041 cttcttccgt ccagaccggg ccaccattgg cgtcgatcaa ttgatccaca ccgcaggaga
    14101 tacagtcgtc gatcagctcc agcactttgc catacgggtt aaagtacagt cccagcttgg
    14161 ctttgttcgg taacttttca tgtaaatatt tgattggcga tggaatattc agcagcagta
    14221 gacggcgaag accgttccac attgcctgct tttgctccag cgggttatca aacagtttga
    14281 tcgccacact gtcgcgctca tccaccagcg ccggccacgc cttcactttg tagttgccac
    14341 gcttctgttc gtagctttcc ggcagctgac caaaactcca gatatgtaag ccgctctgct
    14401 cgataccgtc atccgccacc gcagatagcg tttcctgcac tttgcctttc agcgcatctt
    14461 tcagatcttg tagcgagcgc ccttctttta gcttcttgtt tttgtcatcc accacgcgga
    14521 aggtaatttt caggtgatca ggcacctgat cccagtgcca gtcttcgcgg tcaacggtaa
    14581 cgccggtcat ccgccgtaac tcgcgctcaa ggctgtcgag caacggtaac tccagcggtt
    14641 tgacgcggcc taaaaacgct tcggcatagt ttggcgcggg tacaaaatta cggcgtaccg
    14701 gtttcggcaa cgatttaatc agagcaatca ccagttcgcg gcgcagaccg gggatctgcc
    14761 attcaaaccc gctttcctca acctggttaa gtaacggcag cggaatatgt acggtcacac
    14821 cgtcagcatc cgcgccgggc tcaaactgat agctcaaacg cagcttgaga ttgccctgat
    14881 gccagaagtt cgggtaatcc agcttgctga ttttttctgc gccctctttg atcaacatgc
    14941 ttttttcaaa gttgagcaaa tcaggggttt cgcggctgac ttttttccac cagctgtcga
    15001 agtgacgagc ggagattaca tcgtggctga tgcgctggtc gtagaactca aacaacgttt
    15061 cgtcatcaac cagaatatcg cggcgacgtg atttgtgttc cagctcttcc acttcggccc
    15121 gcagtttcag gttttcacgg aagaatgcgt gacgcgtctg ccagtcacct tccaccagcg
    15181 catggcgaat aaagagttca cggcataacg ccggatcgat ctggctgtag ttgaccttgc
    15241 gcgcggcaac aatcggcaaa ccataaacag tgactttttc cgttgccatc accgcgccct
    15301 gcgcccgttc ccagtgaggt tcgctgtagg tgcgtttaat caaatgctga gcgacaggct
    15361 ccacccattc cgggtcgata cgcgcagcaa tgcgccccca caggcggctg gtttctacca
    15421 gttccgccac cattacccat ttcggcggtt ttttgaataa accagaaccg ggaaagatgg
    15481 agaaacgcgc gttacgtgcg ccggtatatt cttgtttatc ggcatctttc atgccgatat
    15541 gggaaagtaa acccgtcagc aacgcaatgt gaatttcgcg atactccgcc ggttcgctgt
    15601 taaccggaat gccaagttct ttcaccacct ggcgcaactg ggtgtagata tcctgccatt
    15661 cgcgcacgcg cagatagttg agataatcga tacgacacag gcgacggaag gcgttggaag
    15721 aaagcgcctt ttgctgctcg ccaagataat tccacagatt cacaaacgcg aggaagtcag
    15781 actctttgtc gtggaagcga cgatgttttt cgtccgatgc ctgctgttta tccatcggac
    15841 gctcgcgcgg atcctgaatg gaaagcgcgg acgtgataat catcgcctca cgcacgcagc
    15901 catgtttttg cgcttccagc accatacgcg ccagacgtgg atcgacaggc aactgcgaga
    15961 gctggcgacc gagcggcgtc agtttatagg cgctggcctg ttcatccgtg gtgatcgcgc
    16021 ccagttcttc gagcagacgc acgccatcct ggatattgcg tttatccggt gcttcgacaa
    16081 acgggaacgc agcgatatcg cccagcccca gcgcggtcat ctgcaaaata accgaggcca
    16141 ggttggtacg cagaatctcc ggatcggtaa actccgggcg cgaaaggaaa tcgtcttcgg
    16201 aatagagacg aatacagatc ccttcggaca cacgcccaca gcggccttta cgctgattgg
    16261 cagacgcctg cgaaatgggt tcaatcggca aacgctgcac tttggtgcga tagctgtagc
    16321 ggctgatacg tgctgtaccg gggtcgataa cgtatttaat ccccggtacg gtcagcgagg
    16381 tttccgcgac gttggtcgcc agcacaatgc gccgtccgct gtgcgactgg aacacgcggt
    16441 tctgttcgct gttcgaaagc cgcgcataaa gcggcaagat ttcggtatga cgcagattta
    16501 gcttgttcag cgcatcggcg gtatcgcgaa tttcccgctc accactcata aagatcagaa
    16561 tgtcgccagg gctttcctga ctcagttcgt ctacggcgtc aaaaatcgcc tgcaactgat
    16621 cgcgctcggt gtcgtcggct tcttcaacaa tcggtcgata gcgcacttcc accggatagg
    16681 tccgaccgga gacttcgata atcggcgcat tattaaagtg gcgcgaaaag cgttccgggt
    16741 cgatagtcgc ggaagtgata atgattttta ggtcaggacg ccgcggcagc aactctttca
    16801 aatagccgag cagaaaatcg atattcaggc tgcgttcgtg tgcttcgtca ataatgatgg
    16861 tgtcgtactg catcagcagg cggtcttgct ggatctccgc cagcaggata ccgtcggtca
    16921 tcagcttgac catcgtgtta tcactaacgt gatcgctgaa acgcactttg taaccgatgc
    16981 aaccgcccgg ctccgttttc agctcttccg caatacggtt cgccaccgtt cttgccgcca
    17041 ggcgacgcgg ctgggtatgg ccgatcagcc ctttaatccc gcgccccagc tccatacaga
    17101 ttttcggtaa ctgagtcgtt ttaccagaac ccgtttcccc ggcgacgatc accacctggt
    17161 gatcacgaat cgcttcgaga atgtcctgtt ttttctgact aaccggtaaa ttgtcaggat
    17221 aagtaatttc cggtcgtgcc gcttcacgca gcaggacttt ccctgccgcc tggtcaatct
    17281 ctttcgccat ctcctggaaa atggcctgtt gtgcatcagg atttttaacc ttcttaacac
    17341 cgtgcagacg ccgagaaaaa cgcagtctgt cgcgcagcat cagcgaatcc agccgctgct
    17401 gcaaggccgt aaaggtcaat ttttgttgtt ctgtcataac gttagtgggc agtagtctgc
    17461 ctgattaatt ttcttttaaa tgatagatat agagtaccac atcgcggctt tctatgcgtt
    17521 gttcaataaa ttcgaacata ggcttcgatt tattgcgcta tctctgtggc aggattgtga
    17581 ataaagtgtc agcaagcaac ggggcatcgc ccattcaaac atctataagg aaacaccatg
    17641 agcaaggtat tagttcttaa atccagcatc ctggcagggt actctcagtc taatcagttg
    17701 tccgattatt ttgttgaaca atggcgcgaa aagcactccg ctgatgaaat caccgttcgc
    17761 gacctggctg caaatccgat tccggtactg gatggcgaac tggttggtgc actgcgtccg
    17821 agcgatgcgc cgctgactcc gcgtcagcag gaagctctgg cgctttccga tgagctgatt
    17881 gccgagctga aagcccacga cgttatcgtt attgcggtac cgatgtataa cttcaacatc
    17941 tcaactcagt tgaaaaacta ttttgacctg gttgcacgcg caggcgttac tttccgctat
    18001 accgagaacg gtccggaagg tctggtaacg ggtaaaaaag ccatcgttat taccagccgc
    18061 ggtgggatcc acaaagatgg accaacggac ctggtgacgc cgtatctgtc cacgttcctc
    18121 ggctttatcg gcattaccga tgtgaaattt gtcttcgccg aagggatcgc atacggtccg
    18181 gaaatggcag cgaaagcgca gtctgacgcg aaagcggcca tcgacagcat tgttgctgca
    18241 taaccggaca cgatcttgat gtggactgta gttcagtcca catctcaatc cacttacctg
    18301 ttttcccata atttcagcat cgctttgtga tcttccttaa gcacaatatg cgaacgtctg
    18361 gctcgaatat aactaatcgc ttcatcaacg gttttacagt gtccgtaaca taacaaccat
    18421 gccgccacta ccagcgcact gcgcgataat cccaacgcgc aatggaccag aacgctgcct
    18481 tgctcttcgc gtaatgtttc cagcatcgcc acggcctgtc ggagctcccc ctcttccgga
    18541 accaccagat ccagcatcgg tacacagaaa tagagtcgat cttttgtcgc tcgtccccga
    18601 gggaattcaa aggtgacgtc cagaaccgca ttctgtgccg gaatatgtcg tggaaacgcc
    18661 cctaaataaa caccagcagt aattttgctc accggctcca ggcgacgaca aaaccaacgc
    18721 atagacagcc acatcccgat gcgccagggc aatgtcagcc agtaaacggc gggcgggagt
    18781 ttcccctgac tatctttccc tgttgttatc gcgccaagcc caccgtaccc acggccaata
    18841 atgagtagcg ataatactgg ccaacataac cagactgacc accataactg aatcatcacc
    18901 attagctcca tcaacacaat gcacgagccc gcgcctacga catatggcag tgctattttg
    18961 attcgacgtt gatcaggttt ctgataattc caacgacgat cgacgggcac catccagtca
    19021 atcaacatac ctaccgccag ccctgtgatg acatcaataa aatgatgctg ccaggtcgtc
    19081 agagtagaaa tagcgatgag taaaaaccat ccgccacaga ctttacgcca cctctcagcc
    19141 agatgctgac gaaagtgacg ccaaagtagc cagcagagaa taatatgcag cgaaggagac
    19201 tggttataag gcagatcaaa cagttcaagt tgcgaaaata gccatcccgt caccccactc
    19261 acttcaggac ggataaaact aaacttcagc ggatagagca aaaaaccgca gcaggccatt
    19321 accgttgcca gaataagccg gtggacaagt cggcgctgtt cgaatgtcgt gctacaaacg
    19381 aacagcgaaa atccatataa aagatccaga ctccagtaag gaacaatagt ccagggaaga
    19441 aaagggatcg ccgtttccca accgaatacc tgactgggga tatcatggct gttaaggtcc
    19501 tgaaccgcgg tgaactgatt aagagatcca taggtgaaga agaaaaacgg ggccagcaac
    19561 aataaccagc cagcgccttg tagcaatacg ttccgacgtt cagttatcac gacgcaccgc
    19621 catcgaaacc gtaaaaatac cccactcatc aatccgttgt gtgcatttat caaatccggc
    19681 atcacgcacg agtgaatcca tctccccttg cgaacgcacg cgcattaccc acggtttacc
    19741 atctttatga ctggttaaca ccccggcaat catctccagt tgagggtgcc acggctgccc
    19801 ggtgtagatg agaatgccgc ccggttcgat ggcattggca agacctgcga gtgagttttt
    19861 tacctgctca ttttcgggaa aaagctcata caggccagag acaatcgcca gcgtaggccg
    19921 cggagttaac gcgctgagtt cctccgggtt aaacgcatcg ccctgttcaa aacgcacccg
    19981 cccagacatt ccccgttggg caatcatcgc ttgcccctgt gcaacattta actcactgta
    20041 atcacgtaac aaaatatcgc ttacggcagg ctcgtttgcc agcgcatcca gtacatagcg
    20101 cccatgccct gcggcaatgt caaccacgcg gacggctaaa cctttggcgt ggagatcggc
    20161 aacggcttgt ttaatcagta tttgtaaatg ggttttacgc tggcgaatac cgcgccagcc
    20221 aacactgttc aggtagattt tgtcaattaa gcgcccgaat gcgttactgc cttgcggttg
    20281 attttgatag acataatcca gggaactgcc tgaatcaaag ccggtgctca ttccgagatg
    20341 caggcccgca gagtgcgccc cgaacaattt catcgccttt cgcataaagc gatacgccaa
    20401 atcaaccggc gataatggca cgggtccacc tgaaaggagc cgccagcgat ccgctgatgg
    20461 tccagtgcgg tcttcatgct gataatcaaa tttttgtgac ttgttagcat ataaacgact
    20521 aataaagctt tgcatttttt caaatgcctg cgccctgttc tcttcaccca acgtgtcgtg
    20581 ataaaagcct ggcagcagat gcagctcttt cagagggcta cgtaatctct gataaaaatc
    20641 aatctgcggt tggcgatgca ccacatagtc atcgcctgat atcagaagtt gcgtggggag
    20701 cgtaatcgcc gccgcatctc taataatacg ttcagacgtt ttgtagagat cgagcaagat
    20761 attaacggca atcgcccgtg tgatcagcgg atcattattg aaactcgcca cccgttgccg
    20821 atcgtgggtc aaatagcgtc ctttcacata ggaattaata aaaaacagac cacgcagacg
    20881 atgccataac gccagcgcag gacgtgccag cggtacatac aatttaacct taaaggccgg
    20941 agaagccagc accagcccgc gaattgcagg cgcataatca tgaacccatg tggcaaccag
    21001 cactgcgccg acgctttgcg cgatcactac cacctcatcc agtccgacct ggctatcgct
    21061 ggcagcaaaa cgaacaaatt catcgacatc ctgcactgaa cgcgcaagag atgggctata
    21121 accacgcggc cccgaagttt gtccatgccc tcgggcatcc caggcataaa aaacagtatc
    21181 tggcatcgcc agttcatcaa cgagatgttg tagacgacca gaatgttcat gaccgcgatg
    21241 aaataagacg atgacctttt tagccccggg ctgtaaagcg ggccagtgcc gataaaacaa
    21301 cgctgtatta tcactgctgg taaaaaaatg ttccccatgg atgcgtgaat tttccatttt
    21361 ctctattttc cttcagtaac agcagtagcg aataaaataa aaaaataccg gagcggtgaa
    21421 aattaatgag tcaatcctgt caaggagtcc gccatgtcct ggtaatagtt tcccactatc
    21481 tttaacacca atatctcgtt tgatggctga catcacgaca tcgccgcaaa aaccactaat
    21541 accaattaac aaacccgcta ataatgcctg taatgtattt agcggtgtca gcaacggtcc
    21601 aataatcagt gacgcaatca tggtggtgat tacgcccccc accagacctt ccagcgtttt
    21661 tcccgggctg actttgggga ccacttttct tctgccgcag gatttccccc ataaatactg
    21721 tgcaatatcg tttgactcgg ttaatgccaa aagaaataac accagtaacg ccccgccctg
    21781 gatatttgtg gttggcaaca ttaataacca ggcggcatga ctcaaggcga aaacggtcat
    21841 tatccagccc caaaaaatgg cgctcacggt atgcagaaaa ccggaggggt cccccataaa
    21901 aacttgcccg gtggctaata ttagaaaccc agccaaaggt ataaataata gatacagctc
    21961 aaagcaatta aaaccgataa acaaatagtt aagaggaata acccaatata accaccgcgg
    22021 aaaatgtaca gatataagcg tacaatattc tttcaatgcc agaaaactta tcagcgcaaa
    22081 aaacgtcaac gtcatccagc gtggacctga aatcaccagt gaaaaacaaa tgacgatgcc
    22141 ccaccaggta cgaatacgta atgtaacttc atcctctttt ctctccggta accgccatag
    22201 cagaaaatgg tttatcagcg ttgctaaaat taacaacgcg aaaagtgttg ccagagattt
    22261 ttccagcatt atctttcagc gctcataaga agcacactcc gacaacgatt aatcgcagtc
    22321 cagagaagca gaattgaagc aatgctccat aaaagattat tccactgcat ccactgtgga
    22381 taaatggcaa cggccagacc ccacagacca aatattaacg cgcgatcgct tttgccaaat
    22441 ggaccggcat aactgcgaac accgttaatc gtctgcgcga gtaaaccgca aaactcggtc
    22501 aatatggtgc aaaataacat gagtatcacg agtgatgcgt tactttccgg taaaaataaa
    22561 aatggtaaat agagcgcaat atcggaaata acatcaccag tctcattcaa aattgcccca
    22621 agccgtgttt gctgattgca ctcacgcgcc aacatgccat ccagcgcatt gagcgccata
    22681 cggataaaaa gcacgatggg caatagcata aagaggatgg gttgtgccac caacatcaac
    22741 agcaatccgg taagaagaga aagcgccagt gcagcaagag tgatgtgatt cgctgtaacg
    22801 tggtgcttgt aaagccaaaa catcgtcggc cttaacagcg actgaaagag cggttttatc
    22861 tggtagagcg tcattatcaa tccttgataa ttcggtttgc gctatggtgc atcaattccc
    22921 ctgacggaga tatcgcaaca cgccgcttca ttaaaaattc agaatatatc aaggtattgg
    22981 ggactcactg tcaggaataa ttatccccaa ttaatcaaag ggttaagaaa tattacgttg
    23041 tggatcaata caactcatca agttttctca tcgcttgttc ccgtattgcg cgcgtcttac
    23101 cgttaagcgc cagctggcgg actaaatcca gttcagggtt aacaagctct aacgccagca
    23161 aaggatcacg tttcagcctc gcctttaata catcaactgg aaaacgggga ttattcatcg
    23221 ccacaatcca ccactcggga tcaatttctg ccgccagtac tgcaggcggt gtgtggcggt
    23281 tgttaagtgc gccgaacttg aaaaatgtga gattgctttt cgccagtgta ctccaccata
    23341 tacgatccgg ttgttttgcc agtacatcca gttgtgctaa cgcatcgggg tcgagtgcat
    23401 aatagacgcg taatgcttca tcaacctgcg cacgggagct gttttcctgc ctcagaaaaa
    23461 acttcgccca aatatcattg cgtggcgtat tttgctgtaa ttccttgagg acaacagcgg
    23521 gtaaattatc ccaactaact aatgcactct ctaaaaagtc ccagtcttcc aaacgtatca
    23581 gggcggtggc atttaatagc atctgatcat caacttcacc gtggctgata agctcagaca
    23641 aaatcatatc agcgctggtt tcatacgcag ctgaatctgc tttcggatta tttttccgat
    23701 gcgataaagc acgttgccaa attttttgtt tcatcgacgt gctcagatac tgatttttcg
    23761 ccagattact ccacccctca ggatcgttga aacgtgtcaa taaccgttgt agagcagcgt
    23821 cagagtgaat tacctgcata tcccgacagc aaaagtaggc aggggatttt ttctccatcc
    23881 ttttaacaaa attaaactgt aagttatctg gcaatgctaa aaataacaca cgtattagat
    23941 catcgtcatt ctcgaatttt tggtataaat aggctgcaac tttgtcactc tcttgtggcg
    24001 tcatccgcaa taattgacca tggtgtttca tctctgccag cttgaacgct aagcttgtga
    24061 caacattgtt tgatgaatcg ttcgccaact gcattgcctg attgtaggtg agttgtcctc
    24121 cagcaccgac ctgacgaata gaggaatcat cactgcggat atagccatca ataatggatt
    24181 caggcaataa ccacgcatct ttataaagct cctgaatgat catatcccaa tctcttttcg
    24241 ccagagtcat tacgatatcc agagggagat tttcacgtag cgcaagattc aacggaccga
    24301 caccatcagg tagacgactc attcgcgtca ttatttcgac agagagcggt tcacggtcga
    24361 gcaaccccag aacaacaggc tggtaaccca attgctgatc ggtcaactcc agaatctcat
    24421 tgtcaggcat atatctcgcc atactttcaa tcgtttgttc atcattgcta ttaataagtt
    24481 gtttgcgtag gttagctgaa attcctttaa gccttgggag agttcgcatt atggcaagat
    24541 caaatgtcgc aatatctcgt ccttcaaatg cagcaacgtt gccttcatcc agcatttgca
    24601 attcctgcac taactcagga gaaggcttag ggcgaaacag cgcttctttt ttcatctgat
    24661 tacagacatt ccctgccatg cgaatattgc ttcgcttaaa aatttctttc agagcatctt
    24721 catcatttat tagaccccaa taatatgcat caggatttat tatcatcagc gcttttttga
    24781 cattttcagt gttatttttg tcactcgcac ggaccatgaa ttcactattt gtcgaagacg
    24841 caacaacagg tgcaattatg tcattgataa atattccacg cgcattaata ctccccttaa
    24901 tcgaagacgg attatcgtaa tatgagtcga taaataccag tccacttgca tttatcgaac
    24961 cggtaattaa cagtggtgaa tccgtaacta tattcgttgc cgtcacgtta ccgagaataa
    25021 cccatggatt agaaagagag aaagtggaaa tattaccttt gactgtcaaa ttaccaagaa
    25081 caatcaattt ttgattactc tctatattac cttctaccac taaatcgcca cgatgcactt
    25141 tccagtccga tgcagataaa ccgaagggga caagattcac gctactgttg atagcttgtt
    25201 caacttgttg tttatcttca gcggtagccg cagaaaaaaa tggttcaata tcaactgtat
    25261 ttgcaacaca tcgaagttcc gcctgtactg gaagactcag cataaacagg ggaagtaaac
    25321 ctatccagcc gcaaatcctg gacttaaaca tatgcactcc atgccattaa aaaagaaaca
    25381 aaagggaaat gcatctttcc cttttgtata ttcgttattc tcgcgaaata ccatccaacg
    25441 tagacaacac ttcctcagaa agatgcaatt tttcagcagc catattctcc cgtaaatgcg
    25501 caacagaaga cgtccctggg atcagcaaaa tattcggtga acgttgtaac agccacgcca
    25561 gcgccacctg catgggtgtt gcacccaggc tcgcagcaac atccgacagc gtagaggatt
    25621 gcagcggtgt aaagcccccg agcgggaaga acggcacgta ggcaatgcca tcgtgggcca
    25681 aagcatcaat cattgcatca tcagcacggt gcgcgatgtt gtattcgttt tgcacacaga
    25741 caatttcggc aatattgcgc gcctctgcaa cctgcgtcgg tgtgacgttg ctcaggccaa
    25801 tatgttttac caggccttgt tgttgcatct ctgccagcac ggtcaggctg gcctcaatcg
    25861 atccttccgc tgggccatga ccatctccca tcataacgcg caggttaacc acatccagta
    25921 cgtccaggcc gagattacgt agattatcgt gcaccgcttt ttgcaactct gccggagaaa
    25981 aggcgggcaa ccaggaagcg tcctctccac gccgcgcacc aattttagtg acaattgtca
    26041 ggtcgtcaga gtaaggataa agcgcttcgc ggataatctg attggtgacg tgcggaccat
    26101 aaaagtcgct ggtatcaatg tgattgacgc ccaatgccag cgcctcgcgc agcacagtta
    26161 tagcgacgtg gcgatctctt gggggaccaa aaaccccagg acctgccagt tgcatcgcgc
    26221 cataaccaag acggttaacg gattttgtac cgagagtaaa tgtattgctg ctcatcgcac
    26281 cctgccctgt aataaaacgg attaagaaaa atcagtatag aaacgaaaag agataaataa
    26341 tgcgagggcg taaacaagat tactggatga gttgctctat tgtcctgaaa aacacaaaac
    26401 aaaacgcgta aagcgccgca tccggcaata aaaaaaccgc aacctgtatc agcgttgcgg
    26461 tttaggtatc acatgctaaa aattagaatg ttcagtatct ggacgttact gtttcggtcg
    26521 cagattctca tcaaatacca gacgtttcct gttcggttca acttcccgca atggctcaac
    26581 ttgatgcaac gtctgcttac aacgagtcac cagcacctga tactcatgcg taccctgctt
    26641 tttccatgcc agctcctgct cactgagttc acgaatcgct ttcgccgggc tgccgacaat
    26701 caggtaattg gcaggcattt ctgctttggc tttcacaaat gcggatgcgc caacaatgct
    26761 gttctcgcca atcaccgcgc cgtccatcac taccgcgttc attcccacta atgcattgcg
    26821 gcggataatg caaccatgaa ggatagcgct atgaccaata tgtccatctt cttccacgac
    26881 agtatcctgc tcgggaaaac cgtgcataac gcaattatcc tgaatgttcg cgccatcttt
    26941 caccacgata cgaccaaaat cgccacgcag gctggcattt ggcccaacgt aaacgccctt
    27001 gcctagaata acatcgccga tcaataccgc tgtcggatgg acaaaactct cttctggcac
    27061 aaccggagtc agaccatcta tctggtaaat tggcatatcg cctcctgttc aatattcaaa
    27121 ccgccaaaac gttgaaaata caggcttccc ggcgcaggca gttcaccgac cgaggtttca
    27181 cctttttcac taacgaacgc taaagcagca ggcgctacgc gctgataaat gttgatacac
    27241 agctgacgcg ccgtatgccc tgcccagtgc gccggaagca actcctctgg caacaatggg
    27301 tctttaagga cgacacggcg ataaaaatgg atcagtaaaa gctgaatatg aaatgcacgc
    27361 tccggggtta actcgtctgc tgcctctttt aacagcggca ccagcgggcg gaatgactga
    27421 ataaaggttt cgtacatggc attttgttca gttaaatgcc agcactcttc cactctggca
    27481 cgcagtgctg cacgagaaag cgccagtggt atttgcgctt caaaacaaat cacgttatcc
    27541 gccacccccg cttcatgcaa aagtgtctgt acatccgcca gtttttgcga cggcgatgcc
    27601 atcaggctgg gtgccagtgc gccaaaacct tgccagatca actgcttttt gacatcagcc
    27661 agcgttgatt tatctaaacc ttccgagagc aacaggagcc atttaccatc ccatgcaggt
    27721 tgctctgcgc gataaatttt actttctgcc cgtcgcgtca ggcgcaagcc tttatcactg
    27781 aggctataga aactgcgtcg cccgatgcgg gaaacatcca gccagccttc tttattaaga
    27841 cgaaacaaag cggtgcgcac gaaacgctca ccaaatccca gcccttccag caaagcagcc
    27901 agactaccca gccagatttc accaccacga tgggaaagcg aatcaccata cagagaggag
    27961 atcaaagatg tgccactgac cggaacagcg tttacagcat gttggataaa agtaacaagt
    28021 ttactcattc gattcagtct gttgttattg ttgtgattta tgaatcatag cacaggtcgt
    28081 atcgacttct ggccctggcg ctttacctca ccagggccag acgacacgtc aggcaccaac
    28141 aatattgcgc agatcaaaca cccgacacgc cttgccttct gaacgcggga tactgccaca
    28201 gttaacgatc atcacatcgg tagagatccc caccatcgac ttaatccgat ggcgcagctg
    28261 gtggcacacc tggcaacgct gctcatgcgt caatgtcagg ctgctttctt tcaactccac
    28321 tttcacagaa agtgaatcaa gatgcccccg gcggttcacc tccagttggt aatggggcga
    28381 taaatgttcg aatttgacaa tctcttcttc cagttgtgac gggaagacat ttacaccgcg
    28441 aatgatcaac atgtcatcgc tgcgtccgct gatgcgatcc atgcggcgca tagtccgcgc
    28501 cgttcctggt aacagtcggg ttaaatcacg cgtgcggtaa cgaatgaccg gcaatgcttc
    28561 tttggtcagc gtggtgaata acagttcgcc atgttcacca tcggcaagcg gtgtgccgtc
    28621 atgaggatta acaatttcag gatagaaatg atcttcccaa atggttgggc cgtcggtagt
    28681 ttccagacac tccatcgccc cccccggccc catcacttct gacaggccat aaatatccag
    28741 tgcggtgatc cccagacgcc gttcaatctc tttacgcatg gcctgtgtcc acggctcagc
    28801 accaaacact ccaacccgca gcgagcaacc gctggcatca ccgcccaact gccgctccag
    28861 ctcttcaatc aggttaaggc aataagatgg cgtaaccatg atcatatctg gttgaaaatc
    28921 gcggatcagt tgcgcttgtt tttccgtctg cccaccagac atcgggatca ccgtagcgcc
    28981 cagacgttcg gcaccatagt gcgcgcccag cccaccagta aacaggccgt aaccataggc
    29041 aacatgaatt ttgtctttgg gcgtgccacc tgccgcacgc aaggaacgcg ctacaatatt
    29101 ggcccagtta tcaatatcgt tttgcgtata gccgacaact gtcggtttac ctgtggttcc
    29161 cgaagaagca tgaatgcgca ccacttgttc catcggcacg gcaaaggtgt cgaagggata
    29221 gttgtcgcgc agatcctgtt tggtggtaca gggaaattta cgcagatctg aaagttccct
    29281 gaaatcatca ggatgtacgc ctgctgcgtc aaatttgcgc cgatacatcg ggacattttc
    29341 atacgcgtgt ttgagcgtcc atttcagacg ttgtgtttgc aacgcctgta actcatcaac
    29401 agacgcggtt tcaatcgggt caagctttgt attggttatc attgtagggt actcgcaggt
    29461 tgatatgctc aaacacgctc cagaatcatg gcgatgccct gaccgacacc gatgcacatg
    29521 gtgcacaatg cgtaacgacc gttacgccga tgcagctcat ggctggcagc cagtgccagg
    29581 cgggcaccac tcattcccaa cggatggcct aaggcgatag cgcctccgtt ggggttaaca
    29641 tgtggggcat catcaggcag ccccaattcg cgtagtacac ccaacgcctg ggccgcgaac
    29701 gcttcgttca gttcaatcac gtccatatcg tgaatactca gccccgcgcg ttccagcacc
    29761 cggcgagttg caggcaccgg accaagcccc atcaggcgcg gttccacccc ggcggttgcc
    29821 atggctacga ttcgcgcccg cggtgtcagt ccttgcgctg ctgccatctg ttcactggca
    29881 ataatcaacg cagcggctcc gtcattcacc ccggaggcat tgcctgcggt aatcacccca
    29941 ttggcacgaa atggtgcttt taacccacgt aactgttcca gcgtcgtttc cgggcgcaga
    30001 tgctcatcat gttgtatttc tgttacaacg cctttcttgt ttttcaacac aaccggaaca
    30061 atctcctcag ccagaatgcc tgaggattgc gcttttgccg tacgttgctg actgcgtagc
    30121 gcaaaactat cttgatcttc tcgcgatatt tttaacagtt cagctacatt ctctgccgtt
    30181 tccggcatgc tgtcagttcc aaattgttga gccatgagcg ggttcacaaa tcgccagcca
    30241 atagtggtat caaacatctc agcctgacga gaaaatgcac tggttgcctt gcccataaca
    30301 aacggtgccc gagacattga ctccacgcca ccagcgatca gcaaatcgcc atcgcccgct
    30361 ttaatcgccc gtgcggcgaa ccccagtgcg tccagcccgg aaccacacaa gcggttaatg
    30421 gttgtgccgg aaacactatg cggcagcccc gccagtaacg tcgccatccg ggctacgtta
    30481 cggttgtctt ctcccgcctg attagcacag ccgaggatca catcatcgat acactccgca
    30541 tcgagacgcg ggtttcgcac cagcagctcc cgcaaaggga tagcagccag atcgtcagcc
    30601 cgaacacctg ataatgcccc accatagcgg ccaattggcg tacgaattcc gtcacagata
    30661 aaggcttcac gcatcaggct tctcctgtaa tggtgccgcc gatgcggtga gatttaccgc
    30721 gaaacagcgc aaccgttttt tgttgttggt taacaatttc gatgtcgtaa acaccggttt
    30781 gcttgccctg atgacgtacc tgcgcagtag cagttaaggt gtctccggca aagcctggac
    30841 gcaaaaaatc aatcgtgcag gcagaagcga cggctgccag cccctggcta ttgcaggcgt
    30901 aggcaaaggc agtatcagcc agtgaaaata gctgcccgcc gtggcaactt tgatgaccgt
    30961 taagcatttg tgcagtgacg gtcatggtca ctacagcaaa gccttcatcc attgagataa
    31021 tgtcgatgcc aagcgctttg gcgcaggcat cgttctcata cattgcatgg gcattttgcc
    31081 aggccttatg actcataacc gctctccaga agcgctcgtt ggcgcagcaa tgaacatggg
    31141 cgatagcgtt cttcgccata gtgatgttgt agattttcca ggagccttaa tattcgctgc
    31201 cagccaagtt gcgctcccca ggcaagcggg ccatatggat aattcacccc aagacgcatg
    31261 gcggtatcga tatcctgttc agaggccacg cctttttgca gcgcatcaag ggcttcattg
    31321 atgatcattg ctaccgttcg ccaaatcagc attcctgggt aatctgcaat ttgcagcact
    31381 gttttgccct gctgttgcag gtaataaatg gccttgcggg tcgctgagtc cgggttcacc
    31441 gctgccgcag caatggtcac caccttgccc gccattttgt cgaccactac caccgggcgt
    31501 gccagtcgta ttgccagcgc ctgtgccgtc tcgccttgtg tctcaatcaa taaaacatcg
    31561 tcaatttccg tgacgccgtc acttttcttt tctactttca ttgggctaaa actgtcgctt
    31621 accgcttcca ggccaacaac tgcctcacgt tccgcgcgcc agtcgtacac gcccagccca
    31681 cttttcttgc ccaaccgtcc accaatcacc agttcctgtt gcaccagcga aggtaaaaaa
    31741 cgacgctcct gccagaaagc gttaaacacc gaacaggtga cagcaaaatt gacgtcctga
    31801 ccaatcagat cggttaattc cagcggcccc atcgggaaac cagcgccatc gcgaagtgca
    31861 gcgtcaatca cttctggtgc agcaacctgc tcttccagtg cccgccaggc ctcggaataa
    31921 taaggacgcg ccacacggtt aacgataaat ccaggggtcg aatgacagcg cacaggctgc
    31981 ttaccccaac tcaacgttag ttcacacaac tgctcaacaa cttccgctgc cgttgccagc
    32041 ccactgacca cctccaccaa cttcatcacc ggtgccgggt taaaaaaatg cagccccgca
    32101 acacgttcag gattttttac ctccgcagca atcgcggtta tagagattga cgaagtgtta
    32161 gtggtcaata gcgtttgtgg cgggcaaact tccgccagct gtgcaaagag cgcttttttg
    32221 acttccagac gttcagacgc cgcttcaatg accaggtccg cagctgccag cgcgtgaata
    32281 tcggtcaccg ggatcaggcg tttcaatgtg cgttcacagg tttcagcagt cagttttccc
    32341 cgcgtcacgc gtgaatttag ccgcgcgtgt atcccgtcga ttgcgcgggt cagcgcttca
    32401 gcagaaatgt catacagtaa aacctgatgt ccatgactgg cggcaacttc agcaatgcca
    32461 gcccccatgg tgccgctccc aatcactgcc acagtttgca catttatcat catgctattt
    32521 ccccgtgaac tgcggtgagc gtttagccag gaacgcactg acaccttcac gataatctgc
    32581 gctccgtccg gcaagtcgct gatagtcgcg ttccagatcc agttgcgtat cgagcgtatt
    32641 ggtttcagcg ctatttatcg cttgcttgat aagtccaaga ccaaatgtcg gttgtgtcgc
    32701 cagatgccgt gccagctgtt gcgcggtatc tgccagcgtt tcatcatcaa caacctgcca
    32761 gatcatcccc cattcgtgcg cctgttcagc actcagttga ttccccagca gtgccagccc
    32821 catggcgcgc gctcgcccag caacgcgtgg cagtaaccag gttccaccgc aatcgggtat
    32881 taagcctaac ttactaaacg ccatgacgaa ttttgctgaa cgggcagcaa taacgatgtc
    32941 gcaccccagt gccagtgttg cgcctgcccc tgccgccacg ccattgactg cacagatcac
    33001 cggttttggc agttttgcca ggcgacgtac cagtgggtta tagaaacgtt caactgacat
    33061 ccctaaatcc ggtgcggggc ctgtgggatc aacgttacga tcgttaagat cctgaccagc
    33121 acaaaatccg cgcccggcac ctgtaagtaa caggcaacgg atagtgtcgt cgcgctcgac
    33181 ctgtttcagg cactctgcca gttgtgcgtg catctcatca ttaaaactgt tcaggcgttc
    33241 cgggcggttg agcgttagtg tcatcacgcc cttttctaca tgactgagga tgaattccat
    33301 cattagcgtc ctttaaagtc gggcgtgcgt ttttgtaaga aagcggagat gccttcatga
    33361 cgatcttctg ttgccgccag caaggtgaat aactgtcgct cctgggcaag tccggcttgc
    33421 aaagccactt cctgcgactg gcgcagcgct tgctttgccg cttgtaaggc caacggcgag
    33481 tgacgtgcca ttttcgatgc cagctgtaag gcgtattcga gggttaaatc gctggggaag
    33541 acgtcgctaa ccagcccggc ctgctgtgct tgctgggcgg tgatactttc tccgctcagc
    33601 accattttgc tggctaacga tttaccgaca ctacggatta aacgttgcgt tccgcctgcg
    33661 ccaggcatga tgccgagagt gatttccggc aacccaaaac gcgcgttctc tccggcaacc
    33721 accacatcgc acaacaatgc cagttcgcaa cccgccccaa gcgcgtaacc attgacggct
    33781 gcgataagtg gtttattaaa ggcctgcaat cgcgcccata gctgcggacg tgtatcgttt
    33841 aaggtggccg cgagatcttt ttctgccatt tcgttgagat cggccccagc ggcaaaaaag
    33901 cgtgcattac cggtaatcac acagaccgaa atgctggtat cggtagccgc agcttccagt
    33961 tcatttacca gttgcatcag cagggcatta tttagcgcat tacgtgcggc gggacggtta
    34021 agggtcagca acaatactcg ttgctgacgg ctgacgatca gttcgctcat gccatcccct
    34081 tcgcgtcaaa gtcaaccacc acatcgctgg tcagcggcag tgcctggcaa ctcaacacat
    34141 aacctgcggc cagttcatcc ggttccagac tgtaattggt ttccatcgcc actttgccac
    34201 gcagcacttt gcatttgcag gtcgcacaga cgccgccttt gcaggcatag ggcagatcag
    34261 ccccctggcg caatgccgca tcgagaatgc tttcatcgtc ggcattaagc acgatttccc
    34321 gatcccgccc atcctgacgt acagtcactt tttgtccgtc actttgcacg ttaacgctac
    34381 gtttgacgcg cgtgccaggc gtattaaacc gctccagatg aatggtttta tctggcattc
    34441 ccagtgcttt taaggcggct tccgtctcat ccatcatcgc cgccggacca caaataaatg
    34501 cctcatcata aagacgaaaa ttaatgagcg aggccccaag tgactgtaat ctttcaccgt
    34561 caatacgccc gtgaagcaga tcgctgtcga gggtttcctg actgaaaatg cacaacaact
    34621 gtaaacgctg aggatatttg tctttcaggt ctgccagtgc ctggcgaaac atcatgctct
    34681 ggctggtacg gttaccgtag atcagggtga actgactttc aggctcggtt tgtaaagtgg
    34741 tggcgataat cgccagcatt ggcgtaatac ctgatcctgc tgcaatagcc agatagcggc
    34801 cctggcgttc ggcctggggc tgatagccga aatgcccctg cggcaccatg acctccagcg
    34861 tcataccctg gcggatgtgt tcgcgggcat agcgggagaa acgtccgcct tcaatggctt
    34921 tcaccgccac actaatttcg ccaggcaggt agctgcggca aatggagtaa caacggcgta
    34981 attcttcacc atcaagactg gcttttaagg tcaaatgttg accggggcga aagcgatacg
    35041 cctcctgcaa aggctggggc accgcaaagg taatggtcac cgcatcacgg gtttccggct
    35101 cgacttttgc cactgttaag gaatgaaacg ttgtcatggc atcctcaaat acatttgaaa
    35161 taatcgaaag gttcgcggca actatcgcag cggtacaatg ctttgcaggc cgtggaacca
    35221 aattcactga taagtgtggt atggacgctg gcgcagcgcg ggcaacgtac ttctggcggc
    35281 aaatgggcat ggcaactgtg tccggcgggc gggctaatgc catactgtcg cagacgctca
    35341 cgggcatcgg gggtcatcca gtcggtggtc catgccgggt cgagttgcag cacaacctga
    35401 acgggggtaa acccgtgggt tgtcattgcc tcacgtatcg cgccaatcaa atgttccgtt
    35461 gccggacaac cggaataagt cggcgtaaag ccgatcaccc atccttctcc catctgtgtc
    35521 acattacgca ccatgcctaa atcggtaatg gtcagcaccg ggatctccgg gtccgggatc
    35581 tggctgagta gcgcccatat ctcatgaacc tgtggcggtg caatggtggc cagacgttgc
    35641 atacccatct cctctgttac cattgctgac cgggcaagac acgctggaga tactgcattt
    35701 ctgccagcat gggtccaagg tgttcggtat gcagtccttt tttaccgcca gtgcgatacg
    35761 cctgctcttg cggtacgttc aatgtggctt cgttgattcc ggcaaaaact tcggcttccc
    35821 acgctgcgcg taaagtgcgt ggatcaaccg caataccctc ttcactcagt gcaatatcaa
    35881 tctcgtcggc atcgaacagt tcggcagtaa aacgccacaa cttgtcgatg gcctgctgca
    35941 tcttttgccc tgatacgtca ataccattgc ccagtcgctc cagccagcca cgactaaaac
    36001 gcaggtgata acgcgcttct ttaattgctt tggcagaaat cgccgccagt tgcggatcac
    36061 ggctttccat cagacgggta aagagcgcca catgccatgc atcgatgaaa tactggcgtg
    36121 caatggtgtc ggcaaaattg ccgtttggtt gttcaaccaa caataagttg ctgaactggc
    36181 gctcgtctcg ggtgaaggcc agggtatctt catcaccttc tcccgctaat tcagcggcat
    36241 acgataagaa gttacgtgcc tgacctaata aatcgaggcc aatgtttgcc agtgcgagat
    36301 cgatttccag ttccggtgcg tgaccgcacc attcccccaa ccgctgggag agcaccagat
    36361 agttatcgcc caggcgcaag gtgtaagccg ttaactgatt catttccgac ctcacatgtg
    36421 ctcaatgcca tcagggatgg tgtaaaacgt tggatggcga tagaccttgc tttcagccgg
    36481 atcaaaaaat tcaccgcgtt cttccggttg cgaggcaaca atttcactcg ccttcaccac
    36541 ccaaattgaa catccttcgc tacgacgggt gtaagcatca cgggcatttt ccagtgccat
    36601 ccgctcatcg gcagcatgta aactgccaac atggcggtgt gataagccct gtttgccacg
    36661 cacgaaaact tcgtataacg gccagtaaac attactcatt ttgaatctcc ttatgcgacc
    36721 ttacgggcaa gttgtttttg tgcatgggcc agcgctgctt cccgtaccca ggtaccttct
    36781 tcccaggctt tacgtttggc gtcgagcctc tcctgattac aaattccgcg accgttaatt
    36841 acttcgttaa actcctgcca gtcgatctca ccaaagcggt agtgaccgct ttcagtgtca
    36901 aaatgcagat ccgggtcagg aacggtcata ccgagcattt caacctgtgg aacggtgttg
    36961 tccacgaaac gctggcggag ttcgtcgttg gtgaaacgtt tgattttcca ggcgagactt
    37021 ctggcgctgt ttggcgagtt atcatcgttt ggcccgaaca tcattaaggc tggccaccag
    37081 aaacggttaa tggcgtcttg caacatctgc ttttgagctt cgctgccctg cgccagtgcc
    37141 atgcaggctt caaacccctg acgctggtga aaactctctt ctttgcagat tttcaccatt
    37201 gctctggcat acgggccata agaagttcgg cataacgcca cctgattgac gatcgctgcg
    37261 ccatccacca gccagccgat aacgccgata tcagcccaac tcaatgtcgg ataattaaag
    37321 atggaggaat atttcatccg tccgtcgagc attttttggt agatgtcttc tctggcacag
    37381 cccagtgttt cggcggcgct atagagatac agaccatgac cggcttcatc ctgcactttg
    37441 gctaacagaa tggctttacg ccgcaaggtt ggtgcgcgag tgatccagtt accttcaggc
    37501 aacataccaa caatctcgga gtgcgcatgc tgcccaatct ggcggatcaa tgtctttcgg
    37561 taagcatcgg gcatccagtc ctgcggctcg atagccgttt cctgggctat ccgttgctca
    37621 aagcgttctt cttgggtcac gtcatcacct ttacgattcc agttttcgtg aattaattaa
    37681 aaacacgaat cacgttgttt tatgaaagtt acacaatggt taactaaagt cacaaatttt
    37741 gtatacttga actgtgacag atttcgcact ctattttgat aactcattat ttatcagtgc
    37801 attaatgttt ttagtagttg atatacagat cacgcagtta acatttcgtt aaaagatccc
    37861 ttgcttttta tgattcgcga tttaactatt agcaacagaa atgtgaaaca tctggagagt
    37921 agcgatgcag cagttagcca gtttcttatc cggtacctgg cagtctggcc ggggccgtag
    37981 ccgtttgatt caccacgcta ttagcggcga ggcgttatgg gaagtgacca gtgaaggtct
    38041 tgatatggcg gctgcccgcc agtttgccat tgaaaaaggt gcccccgccc tccgcgcgat
    38101 gacctttatc gaacgtgcgg cgatgcttaa agcggtcgct aaacatctgc tgagtgaaaa
    38161 agagcgtttc tatgctcttt ctgcgcaaac aggcgcaacg cgggcagaca gttgggttga
    38221 tattgaaggt ggcattggga cgttatttac ttacgccagc ctcggtagcc gggagctgcc
    38281 tgacgatacg ctgtggccgg aagatgaatt gatcccctta tcgaaagaag gtggatttgc
    38341 cgcgcgccat gtactgacct cgaagtcagg cgtggcagtg catattaacg cctttaactt
    38401 cccctgctgg ggaatgctgg aaaagctggc accaacgtgg ctgggcggaa tgccagccat
    38461 catcaaacca gctaccgcga cggcccaact gactcaggcg atggtgaaat caattgtcga
    38521 tagtggtctt gttcccgaag gcgcaattag tctgatctgc ggtagtgctg gcgacttgtt
    38581 ggatcatctg gacagccagg atgtggtgac tttcacgggg tcagcggcga ccggacagat
    38641 gctgcgagtt cagccaaata tcgtcgccaa atctatcccc ttcactatgg aagctgattc
    38701 cctgaactgc tgcgtactgg gcgaagatgt caccccggat caaccggagt ttgcgctgtt
    38761 tattcgtgaa gttgtgcgtg agatgaccac aaaagccggg caaaaatgta cggcaatccg
    38821 gcggattatt gtgccgcagg cattggttaa tgctgtcagt gatgctctgg ttgcgcgatt
    38881 acagaaagtc gtggtcggtg atcctgctca ggaaggcgtg aaaatgggcg cactggtaaa
    38941 tgctgagcag cgtgccgatg tgcaggaaaa agtgaacata ttgctggctg caggatgcga
    39001 gattcgcctc ggtggtcagg cggatttatc tgctgcgggt gccttcttcc cgccaacctt
    39061 attgtactgt ccgcagccgg atgaaacacc ggcggtacat gcaacagaag cctttggccc
    39121 tgtcgcaacg ctaatgccag cacaaaacca gcaacatgct ctgcaactgg cttgtgcagg
    39181 cggcggtagc cttgcgggaa cgctggtgac ggctgatccg caaattgcgc gtcagtttat
    39241 tgccgacgcg gcacgtacgc atgggcgaat tcagatcctc aatgaagagt cggcaaaaga
    39301 atccaccggg catggctccc cactgccaca actggtacat ggtgggcctg gtcgcgcagg
    39361 aggcggtgaa gaattaggtg gtttacgagc ggtgaaacat tacatgcagc gaaccgctgt
    39421 tcagggtagt ccgacgatgc ttgcagctat cagtaaacag tgggtgcgcg gtgcgaaagt
    39481 cgaagaagat cgtattcatc cgttccgcaa atattttgag gagctacaac caggcgacag
    39541 cctgttgact ccccgccgca caatgacaga ggccgatatt gttaactttg cttgcctcag
    39601 cggcgatcat ttctatgcac atatggataa gattgctgct gccgaatcta ttttcggtga
    39661 gcgggtggtg catgggtatt ttgtgctttc tgcggctgcg ggtctgtttg tcgatgccgg
    39721 tgttggtccg gtcattgcta actacgggct ggaaagcttg cgttttatcg aacccgtaaa
    39781 gccaggcgat accatccagg tgcgtctcac ctgtaagcgc aagacgctga aaaaacagcg
    39841 tagcgcagaa gaaaaaccaa caggtgtggt ggaatgggcg gtagaggtat tcaatcagca
    39901 tcaaaccccg gtggcgctgt attcaattct gacgctggtg gccaggcagc acggtgattt
    39961 tgtcgattaa tcggtgaatg aagggcaacg gccaatagtt gcccttttat ttcactaagt
    40021 tttgtgacgt tgtcacatta tgcatgatgt gtacatctat tttcagggca cccactgtat
    40081 gaaaagctgg cacacctgcc aaacaacctg gcaggtgcag gcaatcccct ttgcatcagt
    40141 actgataatg tgaacctgac taaaccgccc acagagcgcg gttgctaaca agaacacaac
    40201 atctgacgag gttaataatg ggaagcccct ctctgtattc tgcccgtaaa acaaccctgg
    40261 cgttggcagt cgccttaagt ttcgcctggc aagcgccggt atttgcccac ggtggtgaag
    40321 cgcatatggt gccaatggat gaaacgctta aagaatttgg tgccgatgtg cagtgggacg
    40381 actacgccca gatctttacc ctgattaaag atggcgcgta cgtgaaagtg aagcctggtg
    40441 cgcaaacagc aattgttaat ggtcaacctc tggcactgca agtgccggta gtgatgaaag
    40501 acaataaggc ctgggtttct gacaccttta ttaacgatgt tttccagtcc ggtctggatc
    40561 aaacctttca ggtagaaaag cgccctcacc cacttaatgc gctaactgcg gacgaaatta
    40621 aacaggccgt tgaaattgtt aaagcttccg cggacttcaa acccaatacc cgttttactg
    40681 agatctccct gctaccgcca gataaagaag ctgtctgggc gtttgcgctg gaaaacaaac
    40741 cggttgacca gccacgcaaa gccgacgtca ttatgctcga cggcaaacat atcatcgaag
    40801 cggtggtgga tctgcaaaac aacaaactgc tctcctggca acccattaaa gacgcccacg
    40861 gtatggtgtt gctggatgat ttcgccagtg tgcagaacat tattaacaac agtgaagaat
    40921 ttgccgctgc cgtgaagaaa cgcggtatta ctgatgcgaa aaaagtgatt accacgccgc
    40981 tgaccgtagg ttatttcgat ggtaaagatg gcctgaaaca agatgcccgg ttgctcaaag
    41041 tcatcagcta tcttgatgtc ggtgatggca actactgggc acatcccatc gaaaacctgg
    41101 tggcggtcgt tgatttagaa cagaaaaaaa tcgttaagat tgaagaaggt ccggtagttc
    41161 cggtgccaat gaccgcacgc ccatttgatg gccgtgaccg cgttgctccg gcagttaagc
    41221 ctatgcaaat cattgagcct gaaggtaaaa attacaccat tactggcgat atgattcact
    41281 ggcggaactg ggattttcac ctcagcatga actctcgcgt cgggccgatg atctccaccg
    41341 tgacttataa cgacaatggc accaaacgca aagtcatgta cgaaggttct ctcggcggca
    41401 tgattgtgcc ttacggtgat cctgatattg gctggtactt taaagcgtat ctggactctg
    41461 gtgactacgg tatgggcacg ctaacctcac caattgctcg tggtaaagat gccccgtcta
    41521 acgcagtgct ccttaatgaa accatcgccg actacactgg cgtgccgatg gagatccctc
    41581 gcgctatcgc ggtatttgaa cgttatgccg ggcctgagta taagcatcag gaaatgggcc
    41641 agcccaacgt cagtaccgaa cgccgggagt tagtggtgcg ctggatcagt acagtgggta
    41701 actatgacta catttttgac tggatcttcc atgaaaacgg cactattggc attgatgccg
    41761 gtgctacggg catcgaagcg gtgaaaggtg ttaaagcgaa aaccatgcac gatgagacgg
    41821 cgaaagatga cacgcgctac ggcacgctta tcgatcacaa tatcgtgggt actacacacc
    41881 aacatattta taatttccgc ctcgatctgg atgtagatgg cgagaataac agcctggtgg
    41941 cgatggaccc agtggtaaaa ccgaatactg ccggtggccc acgcaccagt accatgcaag
    42001 ttaatcagta caacatcggc aatgaacagg atgccgcgca gaagtttgat ccgggcacga
    42061 ttcgtctgtt gagtaacccg aataaagaga accgcatggg caatccagtt tcctatcaaa
    42121 ttattcctta tgcaggtggt actcacccgg tagcaaaagg tgcccagttc gcgccggacg
    42181 agtggatcta tcatcgttta agctttatgg acaagcagct ttgggtaacg cgttatcatc
    42241 ctggcgagcg tttcccggaa ggcaaatatc cgaaccgttc tactcatgac accggtcttg
    42301 gacaatacag taaagataac gagtcgctgg acaacaccga cgccgttgtc tggatgacca
    42361 ccggcaccac acatgtggcc cgcgccgaag agtggccgat tatgccgacc gaatgggtac
    42421 atactctgct taaaccatgg aacttctttg acgaaacgcc aacgctaggg gcgctgaaga
    42481 aagataagtg attgtttcag acaaaaaaac ggcaccaggt gccgtttttt acttatgagc
    42541 gaaccagatt aataccgtac acacaccgac ttagtttcac accaaccgtc cagccagtcg
    42601 gggccaaaat cacggcccgt tcctgactgc ttcatcccac caaacggtaa gttagcgtca
    42661 attaaggtat ggctgtttac ccacaccgtc cctgcctgta agcgatcgct atattccaga
    42721 gcctgggaga gattttgcgt ccagacactg gcagttaagc catattccgt gtcgtttgcc
    42781 agttgtaacg cctcttctcc atccgctact cgcaccaggt ttaccaccgg accaaacacc
    42841 tcttcacgag ttaagcgcaa tttagcatcg ggatttacca ccagcgttgg cgcaacataa
    42901 tacccctctc cggctggtcc attcgacccg cgaatcagct ctgcttgctg tgcctgcgca
    42961 tcgtcgagga atgaacacac tttgtcgcag tgcgcacgag aaaccaaagg gttaatctgt
    43021 gcaacaggtg acatccccgg tcccacttgc aacgatttta ccgcctgctc aaatccacta
    43081 accagcgtgt caaacaacgg cgcttcaata taaattcgcg aactggcggc gcatacttgc
    43141 ccttgattca ggaagcttcc ggtcatcaag ccttcaataa cccattgcgg atcagcatct
    43201 tttaatacaa ttgccgggtt tttaccgccc agttccagcg ttacacgcgt taagtgatca
    43261 gcagcagttc tggcaatacc ttttcccgtc gcggttgaac cggtaaaact gattttcgca
    43321 acatgaggat gtgacgtcag ggccgcgccg catacagcac ctgacccggt gacgacatta
    43381 aaaacgccat cagggatacc agcctcgctg gccagttccg ccacgcgcaa catcgtcagt
    43441 ggcgtggttt ccgaaggctt aatcacgatt gaacagcctg ctgccagtgc tggcatcacc
    43501 ttccacatac caatcatcaa cggaaagttc catggcacaa ttcccgccac tacgccaacc
    43561 ggctctttac gcgtccaggc ctgataacgc gccccctggg gtaagggaat cgacaagtcc
    43621 agcgttttac ccgcgatttt ggtcgttaac ccggcggtat aacgcatcca gttcagcgta
    43681 cagcccactt caaaagcacg agaaatggca attgacttgc cttgctccag ggtttccagt
    43741 tgcgccagct cctcactgtg ctgctccacc agatcagcaa aacgtagcag aatacgttca
    43801 cgctctgcgg gtaatcgccc ggcccagcga cgcgagacaa aggcccgcca ggcagacatg
    43861 actgcgttat ctacatccgc ttcgttggca tcagcagtag acgcaatttc ttgcccggtg
    43921 gccggatcaa agatcgccaa tcgtttttca ctttgtgcgg ggcctggacg accatcaata
    43981 taaagaccgt gttgacgatc gagaaactgt tggacctggc ttaatactgc tacatgcggc
    44041 tctgtcatca cttttcctta ttatttaccc agtgtgattt cagtgtacgc agcgacaaga
    44101 aaagtcactt ttttcgctgt gacattcgct ttgtcagctg tgacaagctc cgcaaatcgt
    44161 gacaataacc gcttatgtct ctatgcctga gaagacttat ttcctatagt caggctcatt
    44221 agcttcgttt actgcaacac aaatgcaaca ataaaaatac atttcacaga gcgaaaacgt
    44281 gccatgaacc ccgcagtgga taatgagttt cagcaatggc tttcccaaat caatcaggta
    44341 tgcggaaatt ttaccggaca cctgctgact gagcgttaca cgggtgtact ggatacgcat
    44401 tttgccaaag gactaaagct gagtaccgtg acaaccagcg gggtgaattt atcccgcacc
    44461 tggcaggaag taaaaggcag cgacgatgcc tggttttaca ccgtttttca gcttagtggt
    44521 caggcaataa tggagcagga tgagcgtcag gtgcagattg gcgctggcga tattacgtta
    44581 ctcgatgcct cacgcccctg ttcgctttac tggcaggagt cttctaaaca gatttcatta
    44641 cttttgccac gcactctgct ggaacaatat tttccccatc aaaaacctgt ctgcgcagaa
    44701 agactggacg ctgacttacc catggtgcaa ctcagtcatc gcctgttaca ggagagcatg
    44761 aataatccgg cactttctga aacagaaagt gaagctgcgc tacaggcgat ggtgtgtctg
    44821 ctgcgcccgg tacttcatca gcgggaatct gttcaacctc gtcgtgaacg tcagtttcaa
    44881 aaagtggtta cgttgataga cgataatatt cgcgaagaga tattacgccc ggagtggata
    44941 gccggagaga caggtatgtc agtacgtagt ttgtaccgaa tgtttgccga taaaggtttg
    45001 gtagtcgcgc aatatattcg taaccgtcgt ctcgattttt gtgcagatgc gattcgccat
    45061 gccgcagatg atgaaaaact ggcaggcatc ggctttcatt ggggattttc tgaccagagt
    45121 catttttcaa cggtatttaa gcaacgcttt gggatgacgc caggcgagta tcgacgtaaa
    45181 ttccgctaac taattggcgt tgtgtgtact gcaacgccaa ttagttaaaa cagatatttg
    45241 actatgcatt tttcagggcg aaaggcaatt tgacaggagt ttttgaatat tcaccttgtt
    45301 tcaggaaaat agatcaatcg ctttctcctg tagcgttatc ccgatattgc ggttttgcta
    45361 ttaaaatttt cgcacccatt taccgtttat cccttgtaca tattcccccg gtttggcccg
    45421 agctacgagc ttttgcccgg cgagtttcgc gatatcatct acagacacat tattttgctt
    45481 tgccagttgt tggtaactcg ctttacgttc ggcattaatg tcggttacta atttctctgt
    45541 ttcagcatcc gttttcagcg caaccagata accataaaat gtttcaccta cccgcccctg
    45601 agttctggct tcatccagag tcaatgccat tacattgctg cttaccagcc caacaagaaa
    45661 cgcacaaaaa agtaatgttt tcttcatcat cgcctcagaa aagatcgcta cgagtttcaa
    45721 gcagctcttc gacatctttg tctgccttga tgatgatctc atgctcaatt ttaacgttca
    45781 tattgatagt gatcggttcc ttgggcgcag cgacttcaat acgcggcgta caggcagcca
    45841 gcataaaaga tgacgtcaac gcagccagta aaattttcat tgtttttcct cacactcttt
    45901 tccttgcgga cagtcatttc ccggcagacg tgcgttctgc tccaaccatg cctggagatt
    45961 atcgccaaag cgtaaactgc gccacagcgt aaaaatattc tcttcatgat ggtaattaag
    46021 gtttaccgta ccgcttttac catcaacgcg actggtacct gtaatgttgg cctgcatggt
    46081 taataaaccg agattatcta agttaatttt tgtcgatgac cggctaattt ccatatagcg
    46141 caaccagtta attgctgaac ccgcagtcat attgtctttc accaccgcat ctgcggtgtc
    46201 tttatccagc cgcaaggtca ttggcccgct atttgccagc caaccatctt tcactatcca
    46261 tttttcgctg tttagccata acggcaatgc accactaaat gccccggaca tggcgaattg
    46321 tttcggattg acggcgctaa ccagttcgct ggatgaaaga ttattcagcc gcaacagcgc
    46381 cgggtcatgt tgcggcatac gtaattgttt catcagtacg ttaccgccta acacatcgac
    46441 gctaacatca gtcaacagca agggttcttc ttcagtccac ggataacgcc cttgcaaatc
    46501 agccgtaata tttttcgctg tcaccagatt aatcacttcg gcaattcgca acgtaacggg
    46561 gccgcgagtc cccagatgcc aggctccatc ggcaaaacgg aaaggcagga caaaatcgac
    46621 accgttaacc tgattatctg gcatccaggc actaccgcct ttcaacacgc cgtgtcctcc
    46681 cgcgcggaat ccttgttcag gcgcagcaga aaatgcaacc tgagcataca gttcaccatc
    46741 gcgtaagttc atcttccagt cgggtggcac cagcggctgg aatacagtca gtgattgttt
    46801 tggccaccag gcgttaccgc gcagacgaat accgtcccag cgaccattta cccgaaccgg
    46861 gccaatctca ccagcatgta aatcgccttt aaagaggaaa tacgtcggat cgcgcccatc
    46921 gacgctaaat tttaaggttg atggcggtaa cacactgccg ccagtgaaca gcgtttgccc
    46981 ggcgtccagt gacagcgcgc cgctgaagga gggatgctgt gcgtcacgta cccagacgac
    47041 gggcttgtcg agaattaatc gcggcttttc taccgtcatc gtaccgtatt gtaactggtc
    47101 gaaaccggtg gagaggtcgg tcagcgtaat cgtgctgtca tgccattcac cttttcctgc
    47161 gacatcccag gtggcattca tcggtgtaaa actgcctttt ccccagtagc gccactgcca
    47221 gcggcccgcg tcagggagaa aatcattcgc cagcccatcc atatgcagca cgaaatcgcc
    47281 cagttcattt tcatgcgcct gcaaaatggc ctgcaaacgt ccgtcaacac cacgttgggt
    47341 gacttttaca cccgctaaag gccagcggat ttcatcgatg tccagcgaat cgatgactct
    47401 tcccttcgaa cgtaataacg cgcctggctc aaaggccagc gttgggtcag tcagacttcc
    47461 acttagctgc gcgggtaagc gggcatataa aatgaggtcc gcctgtttcg cttcaccggt
    47521 cagttgcaga ggcagctggc tgttatccat gcttaatttt cctgggccaa aatttagcac
    47581 cgcgttgcct ttacccgctt gcccctgggt cagcacactc agtcgtccgc tgatcagagc
    47641 gttctcaagc cctgcctgcc agttgtcgac tttgacaccc agtcggccac tcaaaggaaa
    47701 acctgcatac ggccaactcc agcgaccatc gcttacggtc aattgttgac gagtaatttg
    47761 ccacggcaaa tcgagcaacg gatcgccgtt atcccgtgcc agcacaatca attgcccgct
    47821 attttcctgc cagtccagct ctgcatccac cagtgacggt tcctgcggca agtttaacgt
    47881 cgcagtagca tgcccactta caggaagtcc atccggcacg agcggcatag taaattcccc
    47941 caccagttta accggcggct gattttcaaa cgcgacgaca tccagttcgc tgactgtaag
    48001 ttgttgccct ttcagctggc cttgaaattt aactttttcg ccctgataac gcagttgctg
    48061 gatatcagag gttaatgcga gagagagttt tccctgccat tcctgccagg gagaaaaaat
    48121 cagtttatcg atattgatcc aggtgttagg cagcatggac tgccactgcg cgagggtttt
    48181 tggagcagct ggcgattgct ccgtctgcgg caatttcgcc agacaagcag aatcaagttc
    48241 taccgtgccg acgttcaata accagcggct gggatgtgaa aggctggcgt tggtgatatg
    48301 cgcaagctga caatctccca ccagataacg gagatcggga atgattaaac ctttacgcgt
    48361 aatgcgtggg ctttcatcta atgcaatacg tgtcccgagc ggtagccaga tgcccgccag
    48421 agtgggaacc cacagcccga gcgtcatcaa cagcgtcaac ggcacaagaa taatcagtaa
    48481 taacagcgcg agaacggctt tatatttacc cagcatgggt agttaatatc ctgatttagc
    48541 gaaaaattaa gcattcaata cgggtattgt ggcatgttta accgttcagt tgaaggttgc
    48601 gcctacacta agcatagttg ttgatgaatt tttcaatatc gccatagctt tcaattaaat
    48661 ttgaaatttt gtaaaatatt tt
//
LOCUS       DAZGLJ010000010        45935 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000010 DAZGLJ010000000
VERSION     DAZGLJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.010"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            13..708
                     /gene="araD"
                     /locus_tag="ACHK49_000526"
     CDS             13..708
                     /gene="araD"
                     /locus_tag="ACHK49_000526"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO9261221.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMEIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            783..3134
                     /gene="polB"
                     /locus_tag="ACHK49_000527"
     CDS             783..3134
                     /gene="polB"
                     /locus_tag="ACHK49_000527"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO9261222.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            3299..6205
                     /gene="rapA"
                     /locus_tag="ACHK49_000528"
     CDS             3299..6205
                     /gene="rapA"
                     /locus_tag="ACHK49_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO9261223.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            6217..6876
                     /gene="rluA"
                     /locus_tag="ACHK49_000529"
     CDS             6217..6876
                     /gene="rluA"
                     /locus_tag="ACHK49_000529"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO9261224.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYVADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(6993..7808)
                     /gene="djlA"
                     /locus_tag="ACHK49_000530"
     CDS             complement(6993..7808)
                     /gene="djlA"
                     /locus_tag="ACHK49_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO9261225.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            8063..10417
                     /gene="lptD"
                     /locus_tag="ACHK49_000531"
     CDS             8063..10417
                     /gene="lptD"
                     /locus_tag="ACHK49_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO9261226.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            10470..11756
                     /gene="surA"
                     /locus_tag="ACHK49_000532"
     CDS             10470..11756
                     /gene="surA"
                     /locus_tag="ACHK49_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO9261227.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            11756..12745
                     /gene="pdxA"
                     /locus_tag="ACHK49_000533"
     CDS             11756..12745
                     /gene="pdxA"
                     /locus_tag="ACHK49_000533"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO9261228.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            12742..13563
                     /gene="rsmA"
                     /locus_tag="ACHK49_000534"
     CDS             12742..13563
                     /gene="rsmA"
                     /locus_tag="ACHK49_000534"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO9261229.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            13566..13943
                     /gene="apaG"
                     /locus_tag="ACHK49_000535"
     CDS             13566..13943
                     /gene="apaG"
                     /locus_tag="ACHK49_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO9261230.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            13950..14798
                     /gene="apaH"
                     /locus_tag="ACHK49_000536"
     CDS             13950..14798
                     /gene="apaH"
                     /locus_tag="ACHK49_000536"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO9261231.1"
                     /translation="MATYLIGDVHGCYDELFALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            14823..15422
                     /locus_tag="ACHK49_000537"
     CDS             14823..15422
                     /locus_tag="ACHK49_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HFO9261232.1"
                     /translation="MADYFEIRADYNQHTITIYQAYNDAIADVAVRDGRFGAPFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQHILAIRIKRTFFDTLLEQAVLTTPEAHVYPHAGI
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNLLINGKTTQAKSFLPPEKIYPVSAAARKALGMKNS"
     gene            15458..15925
                     /locus_tag="ACHK49_000538"
     CDS             15458..15925
                     /locus_tag="ACHK49_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9261233.1"
                     /translation="MRQINTGLNTREIILEMIDEADAPPFAEQRTWHRRRIAFLCDCI
                     SVAVDYGFFCSVSQQSIMNITKMAEDVWITESKTTQLIDIWQQLKQLYPPETIPYAQK
                     LPTKELLELLLSSPDDLRIIEIPDIIGDVFDDQTWIRLFKKHFADVIQLRCDV"
     gene            complement(16024..16203)
                     /locus_tag="ACHK49_000539"
     CDS             complement(16024..16203)
                     /locus_tag="ACHK49_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO9261234.1"
                     /translation="MNDKTFDFTSERDFVSRAHCVGLNLQATLQWNEVNEDAISALNE
                     FAEETGCFSDEYRKF"
     gene            complement(16224..16703)
                     /gene="folA"
                     /locus_tag="ACHK49_000540"
     CDS             complement(16224..16703)
                     /gene="folA"
                     /locus_tag="ACHK49_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO9261235.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(16895..18757)
                     /gene="kefC"
                     /locus_tag="ACHK49_000541"
     CDS             complement(16895..18757)
                     /gene="kefC"
                     /locus_tag="ACHK49_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO9261236.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(18750..19280)
                     /gene="kefF"
                     /locus_tag="ACHK49_000542"
     CDS             complement(18750..19280)
                     /gene="kefF"
                     /locus_tag="ACHK49_000542"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO9261237.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(19388..20719)
                     /gene="yaaU"
                     /locus_tag="ACHK49_000543"
     CDS             complement(19388..20719)
                     /gene="yaaU"
                     /locus_tag="ACHK49_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9261238.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(20778..21065)
                     /gene="fixX"
                     /locus_tag="ACHK49_000544"
     CDS             complement(20778..21065)
                     /gene="fixX"
                     /locus_tag="ACHK49_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO9261239.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(21062..22348)
                     /gene="fixC"
                     /locus_tag="ACHK49_000545"
     CDS             complement(21062..22348)
                     /gene="fixC"
                     /locus_tag="ACHK49_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO9261240.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(22399..23340)
                     /gene="fixB"
                     /locus_tag="ACHK49_000546"
     CDS             complement(22399..23340)
                     /gene="fixB"
                     /locus_tag="ACHK49_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO9261241.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(23355..24125)
                     /gene="fixA"
                     /locus_tag="ACHK49_000547"
     CDS             complement(23355..24125)
                     /gene="fixA"
                     /locus_tag="ACHK49_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO9261242.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNMPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            24599..26113
                     /gene="caiT"
                     /locus_tag="ACHK49_000548"
     CDS             24599..26113
                     /gene="caiT"
                     /locus_tag="ACHK49_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO9261243.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            26144..27286
                     /gene="caiA"
                     /locus_tag="ACHK49_000549"
     CDS             26144..27286
                     /gene="caiA"
                     /locus_tag="ACHK49_000549"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO9261244.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            27415..28632
                     /gene="caiB"
                     /locus_tag="ACHK49_000550"
     CDS             27415..28632
                     /gene="caiB"
                     /locus_tag="ACHK49_000550"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO9261245.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            28706..30259
                     /gene="caiC"
                     /locus_tag="ACHK49_000551"
     CDS             28706..30259
                     /gene="caiC"
                     /locus_tag="ACHK49_000551"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO9261246.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLLTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            30368..31153
                     /gene="caiD"
                     /locus_tag="ACHK49_000552"
     CDS             30368..31153
                     /gene="caiD"
                     /locus_tag="ACHK49_000552"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO9261247.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            31159..31749
                     /gene="caiE"
                     /locus_tag="ACHK49_000553"
     CDS             31159..31749
                     /gene="caiE"
                     /locus_tag="ACHK49_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO9261248.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(31835..32230)
                     /gene="caiF"
                     /locus_tag="ACHK49_000554"
     CDS             complement(31835..32230)
                     /gene="caiF"
                     /locus_tag="ACHK49_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO9261249.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(32491..35712)
                     /gene="carB"
                     /locus_tag="ACHK49_000555"
     CDS             complement(32491..35712)
                     /gene="carB"
                     /locus_tag="ACHK49_000555"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO9261250.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(35730..36878)
                     /gene="carA"
                     /locus_tag="ACHK49_000556"
     CDS             complement(35730..36878)
                     /gene="carA"
                     /locus_tag="ACHK49_000556"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO9261251.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(37334..38155)
                     /gene="dapB"
                     /locus_tag="ACHK49_000557"
     CDS             complement(37334..38155)
                     /gene="dapB"
                     /locus_tag="ACHK49_000557"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO9261252.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            complement(38322..39236)
                     /gene="rihC"
                     /locus_tag="ACHK49_000558"
     CDS             complement(38322..39236)
                     /gene="rihC"
                     /locus_tag="ACHK49_000558"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO9261253.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            complement(39302..40252)
                     /gene="ispH"
                     /locus_tag="ACHK49_000559"
                     /gene_synonym="lytB"
     CDS             complement(39302..40252)
                     /gene="ispH"
                     /locus_tag="ACHK49_000559"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO9261254.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(40254..40703)
                     /gene="fkpB"
                     /locus_tag="ACHK49_000560"
     CDS             complement(40254..40703)
                     /gene="fkpB"
                     /locus_tag="ACHK49_000560"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO9261255.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(40791..41285)
                     /gene="lspA"
                     /locus_tag="ACHK49_000561"
     CDS             complement(40791..41285)
                     /gene="lspA"
                     /locus_tag="ACHK49_000561"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO9261256.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(41285..44101)
                     /gene="ileS"
                     /locus_tag="ACHK49_000562"
     CDS             complement(41285..44101)
                     /gene="ileS"
                     /locus_tag="ACHK49_000562"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO9261257.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(44144..45085)
                     /gene="ribF"
                     /locus_tag="ACHK49_000563"
     CDS             complement(44144..45085)
                     /gene="ribF"
                     /locus_tag="ACHK49_000563"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO9261258.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLGVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(45093..45311)
                     /gene="yaaY"
                     /locus_tag="ACHK49_000564"
     CDS             complement(45093..45311)
                     /gene="yaaY"
                     /locus_tag="ACHK49_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO9261259.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            45414..45677
                     /gene="rpsT"
                     /locus_tag="ACHK49_000565"
     CDS             45414..45677
                     /gene="rpsT"
                     /locus_tag="ACHK49_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO9261260.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        11127 a        12465 c        11804 g        10539 t
ORIGIN      
        1 tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa cctggcgctg
       61 ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga tcgcgagcgc
      121 ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc tgacgatatg
      181 gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc ctcctccgac
      241 acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat tgtgcatacg
      301 cactcgcgcc acgccaccat ctgggcgcag gcgggtcagt cgattccagc aaccggcacc
      361 acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac cgacgcagaa
      421 atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt tgaaaaacag
      481 ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc gtttgcatgg
      541 ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt cgcttatatg
      601 gagatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac gctgctggat
      661 aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg actgtataaa
      721 accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga tggattttca
      781 gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg caagggacag
      841 aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt gcaccgcaag
      901 agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt ttgcagggtg
      961 aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag ccggtgtatg
     1021 gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg cgtgaaggtg
     1081 gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg gagcggttta
     1141 tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt aatgcccgtc
     1201 tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat attgaaacca
     1261 cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc atcgtttata
     1321 tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa tacgtcgcca
     1381 gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat cctgatgtga
     1441 tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat gccgagcgtt
     1501 accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc gagcacggct
     1561 ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac ggtatcgagg
     1621 cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc gctcaggagc
     1681 tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa attgaccgcc
     1741 gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc gagctggtga
     1801 cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca acggtgaacg
     1861 gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc tattttccgc
     1921 gaatgcatcg cgctggttat gtcgcgccta atctaggcga agtgccgccg cacgccagcc
     1981 ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg gtgctggact
     2041 ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc gggctggttg
     2101 aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat gcctggttct
     2161 cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg cgcgatgaag
     2221 ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg aatgcctttt
     2281 atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca tcgtcgatca
     2341 ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg cagggctacg
     2401 acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca cattcggaag
     2461 aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg tgggcggaaa
     2521 cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact catttctgcc
     2581 gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc tacgccgggc
     2641 tgattcagga gggcgacaag cagcggatgg tgtttaaagg gctggaaacc gtgcgcaccg
     2701 actggacgcc gctggcccag cagtttcagc aggagctgta cctgcgtatc ttccgcaacg
     2761 agccatatca ggagtacgtc cgccaaacca tcgacaaact gatggcgggt gaactggatg
     2821 cgcggctggt ttaccgtaaa cgccttcgcc gtccgctgag cgaatatcag cgtaatgttc
     2881 cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa tcaaaagcgc ggtcgcccct
     2941 tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc ccggagccgc
     3001 tggactacca acgttcacca ctggattacg aacactatct gacccgccag ctacaacccg
     3061 tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg accgggcaac
     3121 ttgggctatt ttgagcaaaa aaaagagttc gccagatacc attttgatgc gtgacgaatg
     3181 ctttgccatc cagtaccata gcgccctttc cattcctgga cctgaataac gccactacct
     3241 cataagcacg gtagcgggtg gttattgcct gcaattaaag atatagagcc gaacacatat
     3301 gccttttaca cttggtcaac gctggatcag cgatacagaa agcgaattgg gacttggaac
     3361 cgttgtcgcg gtggatgcgc gaactgtcac tttacttttc ccatctactg gtgaaaaccg
     3421 tctgtacgca cgcagtgatt cccccgtgac ccgcgtgatg ttcaaccctg gtgataccat
     3481 taccagccat gacggctggc agatgcaagt cgaagaagta aaagaagaaa atggcttgct
     3541 gacctatatc ggtactcgcc tggatactga agagtccggc gtagccctgc gtgaagtttt
     3601 ccttgatagc aaactggtgt tcagcaaacc gcaggaccgt ctgtttgccg ggcagattga
     3661 ccgtatggac cgctttgcgc tgcgttatcg cgcgcgtaag tattccagcg aacagttccg
     3721 tatgccgtac agcggcctgc gcggtcagcg taccagcctg atcccgcatc agctcaacat
     3781 cgctcatgat gttggtcgcc gccacgcgcc gcgcgttctg ctggctgacg aagtgggttt
     3841 agggaaaacc attgaagccg ggatgatcct gcatcagcaa ctgctctctg gcgctgctga
     3901 acgtgtgctg attatcgtcc cggaaacctt acagcatcag tggctggtag aaatgctgcg
     3961 ccgtttcaac ctgcgttttg cgctgtttga tgatgagcgt tatgccgaag ctcagcacga
     4021 tgcttacaac ccgttcgaca ccgaacagct ggtgatttgc tcgctggatt ttgcccgtcg
     4081 tagcaaacag cgcctggaac atctctgtga agccgaatgg gacctgctgg tggtcgatga
     4141 agcgcatcac ctggtgtgga gcgaagacgc gccgagccgc gaatatcagg ctattgaaca
     4201 actggcagag cacgtgccgg gcgttctgct gctgaccgca accccggaac agttgggaat
     4261 ggaaagccac ttcgcccgtc tgcgtctgct ggacccgaac cgtttccacg attttgcgca
     4321 gttcgttgaa gagcagaaaa attatcgtcc ggttgcggac gccgttgcca tgctgctggc
     4381 aggtaacaaa ctgagcaatg acgaactgaa catgcttggc gagatgatcg gcgagcagga
     4441 tatcgagccg ctgttgcaag cagcaaacag cgacagcgaa gatgcccaga gcgcccgtca
     4501 ggaactggtt tcgatgctga tggatcgcca cggcaccagc cgcgtgctgt tccgtaacac
     4561 ccgtaacggt gtgaaaggat tcccgaaacg cgagctgcac accattaagc tgccgctacc
     4621 gacgcagtat cagacggcta ttaaagtctc cggcattatg ggcgcacgta aaagtgcgga
     4681 agatcgtgct cgcgatatgc tctacccgga gcgtatttat caggaatttg aaggtgataa
     4741 cgccacctgg tggaacttcg atccgcgcgt tgagtggctg atgggctacc tgaccagcca
     4801 tcgctctcag aaagtgctgg tgatctgcgc caaagctgcc actgcgctgc aactggagca
     4861 ggtactgcgc gaacgtgaag gtattcgcgc tgcggtgttc cacgaaggta tgtcgattat
     4921 cgaacgtgac cgcgctgccg cctggtttgc cgaagaagac accggcgcac aggtactgct
     4981 gtgctcagaa atcggttctg aaggacgtaa cttccagttc gccagccaca tggtaatgtt
     5041 tgacctgcca ttcaacccgg atctgctgga gcagcgtatt ggtcgtctgg atcgtatcgg
     5101 tcaggcgcac gatattcaga tccatgtgcc ttatctggaa aaaactgctc agtcggtgct
     5161 ggtgcgctgg tatcacgaag gtctggatgc attcgagcac acctgcccga ccggacgcac
     5221 tatttacgat agcgtctaca acgatctgat taactatctg gcttcaccgg atcaaaccga
     5281 aggctttgac gatctgatca aaaactgccg cgagcaacat gaagcgctga aagcacaact
     5341 ggaacagggg cgtgatcgcc tgctggaaat ccactccaac ggtggcgaaa aagcccaggc
     5401 actggcagaa agcattgaag agcaggatga cgataccaac ctgatcgcct tcgccatgaa
     5461 cctgttcgat attatcggta tcaatcagga cgatcgcggc gacaacatga tcgtgctgac
     5521 cccgtccgat catatgctgg tgccggactt ccctggcctg tcggaagatg gcatcaccat
     5581 cacctttgat cgtgaagtgg cgctggcgcg tgaagatgct cagtttatta cctgggaaca
     5641 tccgctgatc cgcaacggtc tggatctgat tctttctggc gataccggta gcagcacgat
     5701 ttcactgtta aaaaacaaag cgttgccggt aggtacgctg ttggtggaac tgatttacgt
     5761 ggtcgaagcc caggcgccga agcagttgca gctcaaccgc ttcctgccac cgacgccggt
     5821 acgtatgctg ctggataaaa acggcaacaa cctggcggcc caggtggagt ttgaaacctt
     5881 taaccgccag cttaacgcgg ttaaccgtca caccggcagc aaactggtta acgccgtgca
     5941 gcaggatgtt cacgctatcc ttcagctggg tgaagcgcag atcgagaaat ctgcccgtgc
     6001 attgattgat gccgcacgta acgaagccga cgaaaaactg tctgccgagc tgtctcgtct
     6061 ggaagcgctg cgcgcagtga acccgaacat tcgtgacgac gaactgaccg ccattgagag
     6121 caaccgtcag caggtaatgg aaagcctgga tcaggcaggc tggcgtctgg atgccctgcg
     6181 tttgatcgtt gtaacgcatc agtaacggag ccgaaaatgg ggatggaaaa ctacaatcca
     6241 ccgcaggaac cctggttggt tatcctgtat caggatgacc acattatggt ggttaacaag
     6301 ccgagcggtt tgttgtctgt gccgggtcgt ctggaagagc acaaagatag cgtgatgacg
     6361 cgcattcagc gtgattatcc gcaggcagaa tcggtgcatc gtctggatat ggctaccagc
     6421 ggcgtgattg tagtggcgct gaccaaagcc gcggagcggg agttaaaacg ccagttccgc
     6481 gagcgcgagc cgaaaaagca gtatgtggcc cgcgtctggg ggcatccatc ccccgcagaa
     6541 ggtctggtgg atctgccgct gatttgcgac tggccaaacc gcccgaaaca gaaagtctgt
     6601 tacgaaacgg gtaaacctgc gcagacggaa tatgaagtgg tggagtatgt ggcggataac
     6661 acggcaagag tggtgttaaa accgattacc gggcgttcgc atcaattgcg tgtgcatatg
     6721 ctggcgctgg gtcatccgat tctcggcgat cgtttttatg catcaccaga agcgagagcg
     6781 atggcaccac gtttgttgct acatgcagag atgttgacga ttacccatcc ggcgtatggc
     6841 aatagtatga cgtttaaagc gccagcggat ttttaatcgt cacgctggcg cagttcggta
     6901 ggcggataag gcgttcacgc cgcatccggc aattgtgcgc cgattgcctg atgcgacgcg
     6961 ggccgcgtct tatcaggcct acagtttaaa acttatttaa accctttctg ctgctttatc
     7021 agctcatatg cctgctgaat ttcctgcgct ttctgcttcg ccatctccat catctcaggc
     7081 ggcaaacctt tcgccaccag cttatcggga tggtgttcac tcatcagctt acggtaggca
     7141 cgtttgatgg tggtcgcatc atccgtcggc ttcacgccca gcacattaca ggcatcttcc
     7201 agcgtcgggc cacgctgcgc ttgctgccag ttaccaccgc cagattgctg ctgataaccg
     7261 ccgccaaact gtgcaccgcc ctgcatcatg cgcaaaaact ggtcaaactg ggcgcgggag
     7321 atccctaatt cttcagcaat gacatacagc accgcccgtt cattcgggtg cagtgaacca
     7381 tcagcaaacg ccgcctgaat ctggatctcc agaaacatac gaattaagtc aaaacgacca
     7441 aagcagacac tgcgaaactg gcgcatcttt tcgcgcagcg ggtaattatc tgattttccc
     7501 actcggaacg cgttttgcgc cgcagtacgg gaagcgccat gaaggttcat tcggtccatc
     7561 aactggctgg cgatatgaat atcagcctcc gtgacgcgac ctttggattt ggttaaatgc
     7621 cccatcactt caaaagtggt ggcaaaaaac agcgcctgac gctcacgctg gttggcgaac
     7681 cacgccattt tacggctacg ggctttatca aacatatggc caattaacag gcctaacact
     7741 acgccccaaa agccgccgcc catcagtaag gccacggcca cgccaatgat ttttccccaa
     7801 tactgcatat attccccaaa tcgacacacg gatatcaggg ctatctccca caatataaag
     7861 gtgcttttac cgttttccgg ctgcggtcaa ttgtgggaca tcgcctataa tttgcattat
     7921 catacctgtc attcaatgcc gtgcctaaca ccactgacgc tattcggaca ggattaacac
     7981 tagcgtagag atgacgagta cgttagtctc tgaccgtttg tcacgcgcaa cgttaccgat
     8041 gatggaacaa taaaatcaac gtatgaaaaa acgtatcccc actctcctgg ccaccatgat
     8101 tgccaccgcc ctttatagtc aacagggact ggcagccgac ctcgcctcac agtgcatgtt
     8161 gggcgtgcca agctatgacc gtcctctggt acagggcgat accaatgact tacccgtgac
     8221 tatcaatgct gaccacgcga aaggggacta cccggatgac gccgtgttta ctggcagcgt
     8281 ggatatcatg cagggtaaca gccgtctgca ggccgacgaa gtgcagctcc atcaaaaaga
     8341 ggcaccagga caaccggagc cggtacgtac cgttgatgcg ctcggtaatg tccattacga
     8401 cgataaccag gtgatcctca aagggccgaa aggctgggcg aatctgaaca ccaaagatac
     8461 caacgtctgg gaaggtgatt accagatggt gggtcgccag ggtcgcggta aagcggacct
     8521 gatgaaacaa cgtggcgaaa accgctatac cattctggat aacggtagct ttacctcctg
     8581 tctgccgggt tctgacacct ggagcgtggt aggtagcgaa attattcatg accgcgaaga
     8641 acaagttgcg gagatctgga acgcccgctt taaggtgggt ccggtaccga tcttttatag
     8701 cccctatttg cagttgccgg tgggtgacaa acgtcgctct ggtttcttga tcccgaacgc
     8761 caagtacacc accaccaact actttgagtt ctacctgcca tattactgga acatcgcgcc
     8821 aaatatggat gccaccatca cgccgcatta tatgcatcgt cgtggcaaca tcatgtggga
     8881 gaacgaattc cgctacctct cccaggcggg cgctggcttg atggaactgg actatctgcc
     8941 ttcagataaa gtctatgaag atgaacaccc gaacgatgac agttcacgtc gttggttatt
     9001 ctactggaac cactccgggg tcatggatca ggtgtggcgt ttcaacgtcg actacaccaa
     9061 ggtcagcgat cctagctact tcaatgattt cgataacaag tacggttcca gtactgacgg
     9121 ctacgcaacg caaaaattca gcgttggcta tgcggtgcaa aacttcaatg ccaccgtttc
     9181 aaccaagcag ttccaggttt tcagcgaaca gaacaccagt agctactcgg cagagccgca
     9241 gttagacgtt aattactacc agaatgatgt tggtccattt gatacgcgta tttacggcca
     9301 ggcagtgcac tttgttaaca ccagagacga catgcctgaa gcaacccgtg ttcacctgga
     9361 accgaccatc aatttgccgc tctctaataa ctggggcagc atcaataccg aagcgaagtt
     9421 gctggcaacc cattatcagc aaaccaatct tgactggtat aactccagaa acacgaccaa
     9481 gctggacgaa tccgttaacc gcgtaatgcc gcaattcaaa gttgacggca aaatggtctt
     9541 tgaacgcgat atggaaatgc tggctccggg ttatacccaa acgctggaac cgcgcgcgca
     9601 gtatttgtac gtgccgtatc gcgatcagag cgacatctat aactacgact cgtctctgct
     9661 gcaatctgac tactctggcc tgttccggga ccggacttac ggcggtcttg accgtattgc
     9721 ctccgctaac caggtgacga ccggtgtcac atctcgcata tatgatgatg ctgccgttga
     9781 acgttttaat atttccgttg gtcaaatcta ctatttcacg gagtctcgca ctggcgatga
     9841 caacataaca tgggagaatg acgacaaaac gggttcactg gtgtgggcag gcgatactta
     9901 ctggcgtatc tccgagcgtt ggggattgcg tggcgggatt cagtacgata cacgtctgga
     9961 taacgtagcg accagtaact ccagcattga ataccgtcgg gatgaagacc gtctggtaca
    10021 gctgaattac cgttacgcca gcccggaata tattcaggct acgctgccta agtactattc
    10081 cactgctgag caatataaga atggtatttc gcaggtaggt gctgtcgcca gctggccaat
    10141 tgccgatcgt tggtccattg ttggggccta ctactacgac accaatgcga acaagcaagc
    10201 cgactctatg ttaggtgtgc aatacagctc ctgctgctat gcaattcgcg tcggttacga
    10261 gcggaagctg aacggttggg ataacgataa acaacatgcg gtatatgaca acgcaatcgg
    10321 ctttaacatc gaacttcgcg gcctgagctc caactacggt ctgggtacgc aagagatgct
    10381 gcgttcgaac attctgccgt atcaaaacac tttgtgattt gttgatttac cacgtaatcc
    10441 gcagtgcggt taattgaaat ggaaaaagta tgaagaactg gaaaacgctg cttctcggta
    10501 tcgccatgat cgcgaatacc agtttcgctg ccccccaggt agtcgataaa gtcgcagccg
    10561 tcgtcaataa cggcgtcgtg ctggaaagcg acgttgatgg attaatgcag tcggtaaaac
    10621 tgaacgctgc tcaggcaagg cagcaacttc ctgatgacgc gacgctgcgc caccaaatca
    10681 tggaacgttt gatcatggat caaatcatcc tgcagatggg gcagaaaatg ggagtgaaaa
    10741 tctccgatga gcagctggat caggcgattg ctaacatcgc gaaacagaac aacatgacgc
    10801 tggatcagat gcgcagccgt ctggcttacg atggactgaa ctacaacacc tatcgtaacc
    10861 agatccgcaa agagatgatt atctctgaag tgcgtaacaa cgaggtgcgt cgtcgcatca
    10921 ccatcctgcc gcaggaagtc gaatccctgg cgcagcaggt gggtaaccaa aacgacgcca
    10981 gcactgagct gaacctgagc cacatcctga tcccgctgcc ggaaaacccg acctctgatc
    11041 aggtgaacga agcggaaagc caggcgcgcg ccattgtcga tcaggcgcgt aacggcgctg
    11101 atttcggtaa gctggcgatt gctcattctg ccgaccagca ggcgctgaac ggcggccaga
    11161 tgggctgggg ccgtattcag gagttgcccg ggatcttcgc ccaggcatta agcaccgcga
    11221 agaaaggcga cattgttggc ccgattcgtt ccggcgttgg cttccatatt ctgaaagtta
    11281 acgacctgcg cggcgaaagc aaaaatatct cggtgaccga agttcatgct cgccatattc
    11341 tgctgaaacc gtcgccgatc atgactgacg aacaggcccg tgtgaaactg gaacagattg
    11401 ctgctgatat caagagtggt aaaacgactt ttgctgccgc agcgaaagag ttctctcagg
    11461 atccaggctc tgctaaccag ggcggtgatc tcggctgggc tacaccagat attttcgatc
    11521 cggccttccg tgacgccctg actcgcctga acaaaggtca aatgagtgca ccggttcact
    11581 cttcattcgg ctggcattta atcgaactgc tggatacccg taatgtcgat aaaaccgacg
    11641 ctgcgcagaa agatcgtgca taccgcatgc tgatgaaccg taagttctcg gaagaagcag
    11701 caagctggat gcaggaacaa cgtgccagcg cctacgttaa aatcctgagc aactaatggt
    11761 taaaacccaa cgtgttgtga tcactcccgg cgagcccgcc gggattggcc cggacttagt
    11821 tgtccagctt gcacagcgtg agtggccggt cgaactggtt gtttgtgccg atgccactct
    11881 ccttaccgac cgggcagcga tgctcggttt gccgctcacc ctccgccctt attcccccaa
    11941 ctcccctgca caaccgcaaa ctacgggcac attaacgcta cttcctgtcg cgctacgtga
    12001 atctgtcact gcggggcagt tagcgattga aaatggacat tacgtggtgg agacgctggc
    12061 gcgagcgtgt gatggctgtc tgaacggtga atttgctgcg ctgatcacag gccccgtgca
    12121 taaaggcgtc attaacgacg caggcattcc gtttaccggt cataccgagt ttttcgaaga
    12181 gcgttcgcag gcgaaaaaag tggtgatgat gctggcgacc gaagaacttc gcgtggcgct
    12241 ggcaacgacg catttaccgc tgcgcgatat cgcagatgct atcacccctg cacttttgca
    12301 cgaagtgatt gctattttgc atcacgattt gcgtacaaaa tttggtattg ccgaaccgcg
    12361 cattctggtc tgcgggctga atccgcacgc gggcgaaggc ggtcatatgg gtacggaaga
    12421 gatagacacc attattccgg tgctcgacga gctgcgggcg caggggatga aactcaacgg
    12481 gccgctgcct gccgataccc tgtttcagcc gaaatatctc gataacgccg acgccgtgct
    12541 ggcgatgtac cacgatcagg gtcttcccgt gctaaaatac cagggcttcg ggcgcggtgt
    12601 gaacattacg ctgggcctgc cctttattcg cacatcagtg gaccacggca ccgcgcttga
    12661 actggcggga cgtggcgaag ccgatgtcgg cagttttatt acggcgctta atctcgccat
    12721 caaaatgatt gttaacaccc aatgaataat cgagtccacc agggccactt agcccgtaaa
    12781 cgcttcgggc aaaactttct caacgatcag ttcgtgatcg acagcattgt ttctgccatt
    12841 aacccgcaaa agggccaggc aatggtcgaa atcggccccg gtctggcggc attgaccgag
    12901 ccggtcggcg aacgtctgga ccagctgacg gtcatcgaac ttgaccgcga tctggcggca
    12961 cgtctgcaaa cgcatccatt cttaggcccg aaactgacga tttatcagca ggatgcgatg
    13021 acctttaact ttggcgaact ggccgagaaa atgggtcagc cgctgcgtgt tttcggcaac
    13081 ctgccttata acatctccac gccgttgatg ttccatctgt ttagctatac tgatgccatt
    13141 gccgacatgc actttatgtt gcaaaaagag gtggtgaatc gtctggttgc aggaccgaac
    13201 agcaaagcgt atggtcgatt aagcgtcatg gcgcaatact attgcaacgt gatcccggta
    13261 ctggaagttc cgccgtcagc ctttacacca ccacccaaag tggattccgc cgtcgtgcgc
    13321 ctggttcctc atgcaacgat gcctcacccg gttaaagatg ttcgtgtgtt gagccgcatc
    13381 accaccgaag cctttaacca gcgtcgtaaa accattcgta acagcctcgg caacctgttt
    13441 agcgtcgagg tgttaacggg aatggggatc gacccggcga tgcgagcgga aaatatctct
    13501 gtcgcgcaat attgccagat ggcgaactat ctggcggaga acgcgccttt gcaggagagt
    13561 taacgatgat caattcgccc cgagtgtgta ttcaggttca aagcgtctac attgaggcac
    13621 aatcttcacc tgataatgaa cgttacgttt ttgcttatac cgtaaccata cgcaatctgg
    13681 ggcgagcgcc agtgcagttg ttggggcgtt actggctgat caccaatggc aatggccgtg
    13741 aaaccgaagt ccagggcgaa ggagtggttg gcgtccagcc acttatcgcg cctggcgaag
    13801 agtaccagta caccagcggt gcaatcattg aaaccccgct gggcaccatg cagggtcact
    13861 acgaaatgat cgatgaaaat ggcgtccctt tcagcatcga cattcccgta ttccgactcg
    13921 ccgttcccac actcattcat taaaagaata tggcgacata ccttattggc gacgttcatg
    13981 gttgttacga tgaactgttc gcattgctgc ataaagtaga atttacccct gggaaagata
    14041 ccctctggct gacgggcgat ctggtcgcgc gcgggccggg ttcgctggat gttctgcgct
    14101 atgtgaaatc cttaggcgac agcgtacgtc tggtgctggg caatcacgat ctgcatctgc
    14161 tggcggtatt tgccgggatc agccgcaata aaccgaaaga tcgcctgaca ccgctgctgg
    14221 aagcgccgga tgccgacgag ctgcttaact ggctgcgtcg ccagcctttg ctgcaaatcg
    14281 acgaagagaa aaagttggtg atggcccacg cagggatcac gccgcagtgg gatctacaga
    14341 ccgccaaaga gtgcgcgcgc gatgtagaag cggtgctatc gagtgactcc tatcccttct
    14401 ttctcgacgc tatgtacggc gatatgccaa ataactggtc accggaattg cgggggctgg
    14461 gaagactgcg ttttatcacc aacgccttta cccgtatgcg tttttgcttt ccaaacggtc
    14521 aactggatat gtacagcaaa gaatcgccgg aagaagcccc tgccccactg aaaccgtggt
    14581 ttgcgattcc tggccctgtc gctgaagaat acagcatcgc ctttggtcac tgggcatcgc
    14641 tggaaggcaa aggtacgccg gaaggtattt acgcgctgga taccggctgc tgctggggtg
    14701 ggtcattaac ctgcctgcgc tgggaagata agcagtattt tgtccagccg tcgaaccggc
    14761 ataaggattt gggtgaggga gaggcggtcg cgtcttaacg aattaccatt cagggaggaa
    14821 taatggctga ctattttgaa atacgtgctg attacaatca gcacaccatc acgatttatc
    14881 aggcttacaa tgatgcaatc gctgacgtcg ccgtacgtga cgggcgattt ggcgcacctt
    14941 tttcattcaa ccgcatgacc tggattaaac cctcttttat gtggatgatg gagcgtagca
    15001 actggggact gaaaaaagat caacaacaca ttctcgcaat ccgaatcaaa cgcaccttct
    15061 ttgatacatt gctggaacaa gcggtgctaa ccacgccgga agcccatgtg tatccacatg
    15121 caggaatttg ggaaacgtta tttgcacaag caaatgtgta tgttcagtgg gacccagaac
    15181 gcagcataaa tggtaaaaaa ctggagcacc gatcattaca gcttggtatt tctcgtaact
    15241 tgatcagtca atttaacgaa gacgcgattg ttgctattga tgatctgacg ccgctggtgc
    15301 gaaagtgtca taaccttttg ataaacggca aaacaacaca ggcgaaatcc ttcctgccgc
    15361 cagaaaaaat ttacccagtt agtgcagcag cgagaaaggc tttgggaatg aaaaattcct
    15421 gagacaaata cgcacctatt aagcggaaaa tatctaagtg cgacaaatca acaccgggct
    15481 taataccaga gaaattattc tggaaatgat tgacgaagca gatgcccccc cgtttgctga
    15541 gcagcggacc tggcatcgca ggcgaatcgc ttttctatgt gattgcatca gcgttgctgt
    15601 cgactacggc ttcttctgtt ctgtctctca gcagtcaata atgaacataa ccaagatggc
    15661 tgaagatgta tggataacag aaagtaaaac gacccaactc attgatattt ggcaacaact
    15721 taaacagctt tatccgccag aaactattcc ctacgcccag aagttaccca ctaaggaatt
    15781 gcttgaactg ctactttcta gtcctgacga tcttcgaata attgaaattc ctgacatcat
    15841 cggtgatgtt tttgatgacc aaacctggat tcgattattc aaaaaacact ttgctgatgt
    15901 tattcagcta cgatgtgatg tgtaacaatc caaaatatat cgccagacga tagaccaaaa
    15961 agaaagtatt tagcagatac tcaacattct gacttctgta cagaaatagc agagtttaaa
    16021 aacttaaaac ttccggtact catcagagaa acatccagtt tcttctgcaa attcatttaa
    16081 tgccgagatg gcatcctcgt ttacttcatt ccactgtaga gtggcctgaa ggtttagacc
    16141 tacgcaatga gcgcgactca caaaatcgcg ctcactggta aaatcaaacg tcttatcgtt
    16201 caatttgtag tcctggcaaa aaattaccgc cgctccagaa tctcaaagca atagctgtga
    16261 gagttctgcg catcagcatc gtggaattcg ctgaataccg attcccagtc atccggctcg
    16321 taatccggga aatgggtgtc gccttccact tctgcgtcga tatgcgtcag atacagtttc
    16381 tgcgcttttg gcaggaactg ctcataaacg cgaccgccgc caatcaccat gatttctggt
    16441 acgtcaccac acgccgcgat ggcttcatcc accgacttca cccacgttac gcgatcgtcc
    16501 gtacccggtt gactgctgag gataatattt ttgcgtcctg gcaacggacg accgattgat
    16561 tcccaggtat ggcggcccat aatcacgggt ttatttaagg tgttgcgttt aaaccaggcg
    16621 agatcggcag gcaggttcca cggcatggcg ttttccatgc cgataacgcg atctaccgct
    16681 aacgccgcaa tcagactgat cattgagatt tcccgataaa aaaaattgtc gccactatac
    16741 gtaaagcgta aaccgtcgtc gactggtgcg aggatgatgt tgaggaaaat tttatattct
    16801 gctggcgagt ccacgctctc tccctggact cgccgcatta caatgaaaca aaaacaaaca
    16861 gttagctgta aagtgtgatt tacgtcactc tttattagga tgagggtttc gtttccggtt
    16921 catccgccat gttgccggta tgtttacctt cttcggttcc ctgccagcca tgtcgttgaa
    16981 ttaatgacag atgttcgcgg tcctcggtaa tgatctcact taacatcgcg ctggtgcgtt
    17041 tatagaccgc cgcgcgggct ttggtgtcgt tctcaaccat tgccatctct tccaccatct
    17101 gaatattaaa gcggcggaac acatcggcac gttctcgcgc ttcatacggc cccagaccta
    17161 aactttccag tgccagacgc ccggttttca gcgcaccttc gaaggtttca cgctccggct
    17221 tttcaacgcc tgcctgacgc aaacgaatgt agtggtcgac atcgcgggcg cgggcaataa
    17281 tctgcaaatg cgggaaatgt tctttcacca tctccgtcag ttgcaggttg gtttgcggat
    17341 catcaatagc attaatcagc acttccgctt tcgccgctcc ggcagattcc agtaaatcca
    17401 tccgcgttgc atcgccataa aacactttca taccaaattt acgcagcgtt tcgatatggt
    17461 ccggatcgtg atcgagcacc accattttca ccccgctgga gagcagtaaa cgtccggtaa
    17521 tctgcccaaa acgaccgaat ccggcgataa tcacgcgcgg ctgttcttcg tcgatctcat
    17581 cggcttcacg cgcttcctca gtagaagatt gctcaaggcg attgaggatc accagcagaa
    17641 tcggcgttgc tgccatcgac agcgccaccg ccagggtcag cgatttcgcc cactccggct
    17701 ccagcacatt cgccatctgc gccgcgccaa ataccacaaa ggcaaactca ctgccctgcc
    17761 ctaacaacac cgcaaaccaa cgacgctgtt tatttggcac ttgcaacggt cgggcaatca
    17821 gccacagcat ggcgattttg atgatgagga aaccgagcag caaaatgaca atgcgcaatg
    17881 ggttttcaag cagcgtgcca aagtctatcg acatgccaac accgatgaaa aacagcccca
    17941 acagcaaacc tttaaatggt tcgatatcgc tctccagcgc atgacggtat tccgagcttg
    18001 ccagcagtac gcccgccaga aacgcgccca tcgccatcga caagccgacc tcttccagca
    18061 gcaaaccaaa gccaaacacg aggaataacg ccacggcact aaacacttcc cgcaagccag
    18121 agcgggctac aaaacgcagc gccggacgcg tgacatagcg ccccagcaat accaccagca
    18181 ccagcgcacc cgccactttt aacgccgaga gagcaaatgc gcccatcgtc gtcgaggcac
    18241 tgctcgttgc cagtagcgga atcatcgcca ccagcgggat cgccgcgata tcctggaaca
    18301 gcagcaccgc aaaggcactg cgacccattt gcgtcaccat cagattgcgt tcattcatcg
    18361 cctgcatggc aatcgccgta gaggagagcg ccagcgtcat gccgatcaat tccgcgacct
    18421 gccagcgcag cccaagtaac atgcagaaca gccccagcag gccgccgcaa atcaccatct
    18481 gcaatgcgcc accgccgaac actgccgcac gcagcttcca cagcctttgt ggatcgagtt
    18541 cgaggccgat aataaacagc atcagcacca ccccaatctc ggcaaagtgc agaatagatt
    18601 cggcatcggt caccagtcgc agcccccacg ggccaataat gcagccggcg atcaggtagc
    18661 caagtaccga tcccagacca agacgtaccg caatgggtac aatcagcgct gccgaaccga
    18721 gataaatcag cgcctgaatc agcgtatggc tatccatgat gggcctcctg ccattccagc
    18781 agacgttgct tatagtgacg cgcctgccct tcgagggttt cgtcgtcaca aataaaggtg
    18841 cagtgcatgg caaacggtgg cagccagttc agtccgcagt agattgccgt cgcctgtagc
    18901 ggctgcgaca gcacatcaaa gcccggatgc gcaccaattt caaaatggct ttccccgccg
    18961 ccggtcgtca ccgcccacag caaatgtttg ccatgcagcg ccgtgccgcc atgaccgtaa
    19021 gcccagccgt gggagaaaac tttatcgatc caaagtttga ggagcggagg aatgctgtac
    19081 cactgcatcg ggtgctgcca gacgatcaga tcggcgcgag acagcgcctc ctgctcggcg
    19141 gcaatatcga tattgaagtc aggatagagt tgataaagag agcgaatttc gacgccttcc
    19201 agcgtccttg cctgttcaag catccgttta ttcgcatggg aatgatgcgg atacggatgc
    19261 gcataaatta taagaatcat aggtagcccg cgtgattacg cctttgtttt cccacaaagt
    19321 gtagtcatat attcacgcgg cttatagtca ataatattga ttcagaaaag agagaaatct
    19381 gaacaattta ccccattctc tgcccgcgga tcgtcatatt gctggtttgc gccagtgaca
    19441 tccctcgagt ctccggggca aacgctacgg aaatcaacaa gccaaacagc gagatacccg
    19501 cccccattag catcgtgtta ctgataccgt aattattgat aaagatcggc agtgcccagg
    19561 tcgaaacaat ggtgccaata cggcttaagg acataatcac gcccacggca gaggcgcgga
    19621 tatctgtcgg gaagagttca ttaggataga gccactgcaa attacccggc ccgccagaga
    19681 aaaaggcata caccgcaaag gccatcacta ccagccagat ccccatatcc gggatcagcc
    19741 ccaaaaccgc cagcgccagc gtcatcatgg caaagctgcc aatcaacaat ggacgccgtc
    19801 cggcagtgtt taaccacagc atcggcggaa tacagccgag cataaagaac aggctaatca
    19861 ccacgttccc cagcgccgcg tttttgccaa cacccaatcc caacaaacca acgatttgcg
    19921 ggccaaaggt gtaaatggcg aacattggga tcacctggca ggtccagatg gcggcaacaa
    19981 acagaacaaa aggaaaatgg cggcgattaa acagatcgcg aaaacgggtt tgctgcggct
    20041 gctcttcatc gaaagccacc ggttcgccaa acagttttat catcatctct tcgcactctt
    20101 ttactcgccc tttgcgtaat aaccagcggg gagattcagg cagttcgaat cgaccaatca
    20161 aaatcaacaa acaggggatc gccgcgctac ccagcatcca gcgccagccg ccttccacat
    20221 cataaagcca gtagccgacc agatcggcgc aggtcgcgcc gacataccac atggcggcga
    20281 taaagctgat ggaaaacgcc cgctgacggg tactggagaa ctcggtgatc attgaggtgg
    20341 cgatgggata atctgcaccg atgacaatgc cgataagtac ccgcatcacc aacagttcga
    20401 cgggggatga aacaaacatc gtcgccaccg atatcacgcc gatggcgatg atatcaatga
    20461 ggaacatttt gcgccgtccg actttatcgg aaatatagcc aaacagcgat gtgccaacga
    20521 acagcccggc gagcgttccc gcgcccaaca agccaatcca gtcagcgtcc agttttagcg
    20581 ccggagtcag ttgctccagc gccacgccaa tcattaccag tacataacca tccagaaacg
    20641 gaccaccgct tccccacagc aaaatgcggc ggtgaattga ggagaatttg agatcgtcaa
    20701 agtttctgga cggttgcatg ggttattcct gtttttttgc aatgagagtc agtacggcgt
    20761 tacgtatcaa accaacatca gccgtaacgg aactccacac caaaggtgcc gcgcgggtat
    20821 tcccactgtt ccagcgccga ccccagcccc agaatgcgac aggtgccgca ctccagacat
    20881 ccggcgtaat cgaagcgcac actgccgtca tcctgcttct tgtacagacc tgcggggcac
    20941 gctttcacca gcagctccag ggcctgttta tcggcatcgg ccttcacaac aatgtgcgga
    21001 tgctcttcat cgacattgaa tttattgacg cccagtttga cgtccacatt gacgggagaa
    21061 gtcataaaac ggtcactcct ttcatgccat ccttgatcag attgatgaag cccactttct
    21121 tgccgtggcg gaggattttc ttgcgcatca gttccggcgc actgccgtca atggtgaaca
    21181 ggtcacgcgc cacgcccacc gccagttccg ggtagccgct aaacatgcgt gggttatcaa
    21241 ggaaggccgg tagtttctgg tacatacgca tatcgcgcag cgggccactc tcaagatgct
    21301 gacgatattc cgcgagtttt tgcttactga aatcgtcgct tttcatcgct gaaagcacgg
    21361 tttttgctgc agcttccccg gcggcaatcg ccagatccat accgcgaatg gtaaaaccga
    21421 ggttcataca cattccggcg gcatcaccgg caatcaatac gccgtcacca accaactccg
    21481 gcagcatgtt gatgcctgct tccggcacta cgtgagcgga atattccacc agcttgccgc
    21541 ccgcgatcag cggtgccact gccggatgct gtttgaaatc ttccagcatt tgcggcaccg
    21601 atttttttgc gtcatgcaga tgatgcagac cacaaaccag ccccagcgac agggtatttt
    21661 cattggtata aaggaagccg ccgcccatca ggccatcggt gggtgatccc gcaaacagac
    21721 aagccgcgcc ctgattaccc tgcaactgaa aacggtcttc gattaccgac ttcggtaact
    21781 cgatcagttc cttcacgcca accgccacat ccgtcggttt aacgcgtttt gccatcccca
    21841 gcttttcggc aaggatggag ttcactccat cagcaaggat caccgttttc gcttcaatca
    21901 catcgccatc ggcttctaca ccgacgactt tgccatcgcg ctgtacgagg ttatcgacgc
    21961 ggatcccggt aattaactgc gcgcccgctt cttcggcctg ctccatcagc caggcatcaa
    22021 atttactgcg caaaacggag taagaacgct gggatggcga ggtttcgtca ccattgcagt
    22081 agtccatagt catcgctgac ttttccgtca taaacgcgag tttttcatgg gtgatcaggc
    22141 gttctacggg ggcggagtcg gcgaaaccag gaataatgtg ttccaggctg tgggcataga
    22201 gacgcccgcc ggtgacgttc ttggcacctg cggaattgcc acgctcgata actaacactt
    22261 gcgccccttc gcgggcgagc accagtgcgg caaccgaacc ggcaagccca gcaccgacga
    22321 tgatggcgtc aaagatatct tcggacataa gagctccctg tcagcggcag ggccaaaatg
    22381 cagccctgcc agagtggatc aacgcgctaa agctgcggtc agcgccggaa ggatcttcac
    22441 ggcgtcacca acaatgccgt aatccgcgta ctggaagatc ggcgcatttt tatctttatt
    22501 gatggcgaaa atggtttgcg atgcattagc accaaccatg tgctggatct gcccggagat
    22561 ccccaccgcc aggtacagtt caggtttcag catcaggttg gagataccga catagcgttc
    22621 gtgctccatc catttttcgt tttccgccac cggacgagaa caggccaact ccgcacctat
    22681 cgccttgcaa agctgttctg ccagcgcaat gttctctttg ctgccaatac cgcgaccgac
    22741 gctgaccacc agacgggctt tgtcgagatc gacgctgttg ctctggcgcg cctgggttgc
    22801 cgtgcgggta atcgccacag ccggagcctg ccactccacg gtgtgcgttt cgccagtgcg
    22861 tgacgcgtct ggctgagccg catcgaacgt gccgctgctg atggtcagta ccgcatacgg
    22921 cgtggcaatg cgttcttcgc caatcgccag accaccgtaa accatgtgtt tcactgtcgc
    22981 tttaccgtcc tgtacgctga cggtgctggc atcgttagac accgccgctt taaggcgata
    23041 acccagtttt gccgccagta atttgccgcg acgggtgttt ggcagcagca ccaggccgtc
    23101 tgcgccgtgc tggcgaatag tgtcagccat gacaccggcg taatcttcga tcatccgatc
    23161 gtccggtttg ccgtttaatt tccagacatg attagctccg agctggattg cctgtacgcc
    23221 gtcggcatca ttgaggacaa aggtgttgat ttgattagct aaagcctgcg caccgttcat
    23281 cagttccggc agacgagaag gggtatcgct gaatacccag acttgagaaa acgtgttcat
    23341 agcatcccct gtaattaaat gactttgcga agattttcag caaatgcggc gatctgttct
    23401 tcgccgtcgc cttcaatcac gatgcgctga cgttcgcgct gtttcggcgc agcaacctgt
    23461 tgttcagacc aggcgtccac ggcgttaaaa ccgatatccg ccgccgacca tacctggact
    23521 ggctttttcg ccgcgccgag aatggctttc atcgaaggaa tttgtgggga gttgatatca
    23581 gtggaaacag caacaaccgc aggcagcgga atgcttaagg tttcggtttc atcttccagt
    23641 tcgcgctcaa cggtgagggt atctgcagtc agggagataa ttttgctgac gccgttaact
    23701 gccggcatat tgaggatttc gcccaccagc agaccaacct gctgggcata aaggtcggaa
    23761 gaaccatcgc cacagaggat caaatcaaag cctgctttct gggcggctgc agccagtgcg
    23821 ctcgccgttt gttgcggcag tgcctgctcg aactggtcat caatcaccac aatcagttca
    23881 tccgggccgc gcgatagcac atctttacgc cctttggcgt tggtcagggc tttaccgccc
    23941 acacttaagg ccgtcacctg cgcctctgct gcctgttgct ttagctggca agccgcttca
    24001 atagcgttca gatcgtattg gcttattttg gcatcggctt tgctgaagtc taatgaacca
    24061 tcagcattat tgaccgcaat atcctgttca tcaggcacgc acttatagca agtaataatc
    24121 ttcattgcat ctccagaaat catgaaggaa acgtcgttca tatcggtggt gttaattaca
    24181 gatatcacgc cctttaattt ggaaataaaa ctttaatcac caatattgaa aatgtcacac
    24241 gcagataaat ccaactttca atattgttaa gttcctcacc aatattgaaa acatcgcgta
    24301 gcaaaaagaa attttcaata ttgctttatg gatcaccaat attgaaagtc agatgctatt
    24361 tttttactca caacacaaca taacaaactg attaataatc aaaattaacg agaaaagtgc
    24421 cctctattca cccgaaacaa aaatgtgata acaatcacag aatgcagctt attgaatacc
    24481 cattatgagt tagccattaa cgcgtccacg aggttaataa taattatatt aaatgttaac
    24541 aaaaataaaa caaacgggaa cgcaaaataa atattcgttt tcacagtgga attaactcat
    24601 gaagaatgaa aagagaaaaa cgggaataga accgaaggtt ttctttccgc cgttaataat
    24661 cgtcggcata ctttgttggc ttacagtcag agatctggat gcagcgaatg tcgttattaa
    24721 tgctgtattc agttacgtca ccaatgtatg gggatgggca tttgaatggt atatggtggt
    24781 gatgcttttc ggttggttct ggctggtgtt tggcccgtat gccaaaaagc gtttaggtaa
    24841 cgaaccacct gaatttagta ccgccagttg gatctttatg atgttcgcct cctgtacgtc
    24901 tgctgccgta ctgttctggg gatcgattga gatctactac tacatctcca ccccgccgtt
    24961 tggcttagaa ccgaactcga caggggcgaa agagttgggg ctggcttaca gcttgttcca
    25021 ctggggacct ctgccgtggg ccacttacag cttcctttca gtcgccttcg cttacttctt
    25081 ctttgtccgc aaaatggaag tgattcgccc cagctcgaca ctggtgccgc tggtaggtga
    25141 aaaacacgcc aaagggttgt tcggcactat cgtcgacaac ttctatctcg tcgccttgat
    25201 cttcgcgatg ggtaccagtc tgggccttgc cacgccgctg gtgaccgagt gtatgcaatg
    25261 gttgtttggc attccgcata ccctgcaact ggacgctatc atcattacct gctggattat
    25321 cctcaacgcc atttgcgtcg cttgcggtct gcaaaaaggg gtacgtatcg ccagtgacgt
    25381 gcgtagttac ctgagcttcc tgatgctggg ttgggtgttc attgtcagcg gtgccagctt
    25441 catcatgaac tacttcaccg attcggtggg gatgttgctg atgtatctgc cgcgcatgtt
    25501 gttctatacc gatcccatcg ctaaaggcgg cttcccgcag ggctggaccg tgttctactg
    25561 ggcatggtgg gtgatttatg ctatccagat gagtatcttc ctcgcccgca tctcccgtgg
    25621 tcgtactgtg cgtgaactgt gcttcggcat ggtgctgggg ctgacagcgt caacctggat
    25681 cctgtggact gtactcggta gtaacactct gctgttgata gataaaaaca tcatcaacat
    25741 tccaaatctg atcgaacagt acggtgtggc gcgcgccatc attgaaacct gggccgctct
    25801 gccactcagc accgccacca tgtggggctt cttcatcctc tgctttattg ccaccgttac
    25861 gctggttaac gcctgctctt ataccctggc gatgtccact tgccgcgaag tacgcgatgg
    25921 tgaagaacca cctctgctgg tgcgtatcgg ttggtcaatt ctggttggca ttatcggtat
    25981 tgttctgctg gcgctcggcg gcctgaaacc gattcaaacc gccattatcg ccggaggatg
    26041 cccgctgttc ttcgtcaaca ttatggtgac gctctccttt attaaagacg cgaaacagaa
    26101 ctggaaagat taattaaccc ccaaaatatc aagaggttga aagatggatt ttaatttaaa
    26161 tgatgagcag gaactgtttg tcgccggtat ccgcgaactg atggccagcg aaaactggga
    26221 ggcctatttt gccgagtgcg accgtgacag cgtctacccg gaacgttttg tcaaagcact
    26281 ggcggatatg ggtatcgaca gtctgctgat ccctgaagag cacggtggtc tggacgcggg
    26341 gtttgttact ctcgccgccg tgtggatgga gctgggacgt ctgggggcac caacctatgt
    26401 gctgtaccag ttgccgggcg ggttcaacac cttcctgcgc gaaggcacac aagagcagat
    26461 cgacaagatt atggctttcc gcggcaccgg taagcagatg tggaactcag cgattaccga
    26521 accgggcgcg ggctccgacg tgggtagcct gaaaacgact tatacccgta gaaatggtaa
    26581 gatttatctt aatggtagta agtgttttat taccagcagc gcctacaccc cgtacatcgt
    26641 ggtgatggcg cgcgacgggg cttctccgga caaacctgtc tacaccgaat ggtttgttga
    26701 tatgagcaaa ccgggcatca aagtgaccaa acttgaaaag ctcggtctgc gtatggatag
    26761 ctgctgtgaa atcacctttg acgacgtgga actggacgag aaagacatgt tcggtcggga
    26821 aggtaacggc tttaaccgcg tcaaagaaga gttcgaccat gaacgtttcc tggtagccct
    26881 caccaactac ggtacggcga tgtgcgcctt tgaagatgcg gcgcgctacg ccaatcagcg
    26941 cgtgcagttt ggcgaggcta ttggtcgttt ccagttgatt caggaaaaat tcgcccacat
    27001 ggcgatcaaa ttaaactcca tgaaaaacat gctgtatgaa gcagcgtgga aagcagacaa
    27061 cggcaccatc acctctggcg atgcagcgat gtgcaaatac ttctgcgcca atgcggcatt
    27121 tgaagttgtg gatagcgcaa tgcaggtgct gggcggtgtc gggattgcgg gcaaccaccg
    27181 catcagccgc ttctggcgtg atctgcgtgt agaccgcgtc tccgggggat ctgacgaaat
    27241 gcagatcctg acgctgggtc gcgcggtgct gaagcaatac cgctaagtcc gtgcgctgcc
    27301 tgatgcgacg ctaatgcgtc ttatcaggcc tacgggagca cgaatgtagg ccggataaag
    27361 cgtttacgcc gcatccggca gtcatgcgct acataaattt ttcaggagct aataatggat
    27421 catctaccca tgccgaaatt cgggccgttg gccggattgc gcgttgtctt ctccggtatc
    27481 gaaatcgccg ggccgttcgc cgggcaaatg ttcgcagaat ggggtgcgga agttatctgg
    27541 atcgagaacg tcgcctgggc cgacaccatt cgcgttcaac cgaactaccc gcagctctcc
    27601 cgccgcaatt tgcacgcgct gtcgttaaat attttcaaag atgaaggccg cgaagcgttt
    27661 ctgaaattaa tggaaaccac cgatatcttc atcgaagcca gtaaaggtcc ggcctttgcc
    27721 cgtcgtggca ttaccgatga agtactgtgg cagcacaacc cgaaactggt tatcgctcac
    27781 ctgtccggtt ttggtcagta cggcaccgag gagtacacca atcttccggc ctataacacc
    27841 atcgcccagg ccttcagtgg ttacctgatt cagaacggtg atgttgacca gccaatgcct
    27901 gccttcccgt ataccgccga ttacttttct ggcctgaccg ccaccacggc ggcgctggca
    27961 gcactgcata aagtgcgtga aaccggtaaa ggcgaaagta tcgacatcgc catgtatgaa
    28021 gtgatgctgc gtatgggcca gtacttcatg atggattact tcaacggcgg cgaaatgtgc
    28081 ccgcgcatga gcaaaggtaa agatccctac tacgccggtt gcggtctgta taaatgcgcc
    28141 gacggctaca tcgtgatgga actggtgggc attacccaaa ttgaagagtg ctttaaagat
    28201 attggcctcg cacatctgct cggtacgccg gaaatcccgg aaggcactca gcttatccac
    28261 cgtatcgaat gcccttacgg cccactggta gaagagaaac tcgatgcctg gctggcggca
    28321 cataccatcg cagaagttaa agaacgcttt gccgaactga atatcgcctg tgccaaagta
    28381 ctgaccgtac cggaactgga aagcaatcca cagtatgttg cccgtgaatc aatcactcag
    28441 tggcaaacga tggatggtcg cacctgcaaa gggccgaaca tcatgccgaa attcaaaaat
    28501 aaccccggac aaatctggcg cggaatgccc tcacatggca tggacacggc tgccattttg
    28561 aaaaatatcg gctacagcga aaacgacatt caggagttgg tcagcaaagg tctggccaaa
    28621 gttgaggact aaacgcgtgg cgtcagtcgg cttgcctgac tagcgcccgc ccaataacaa
    28681 acgaacaaaa atggatagag gtgcaatgga tatcattggc ggacaacatc tacgtcaaat
    28741 gtgggacgat ctggcggacg tttacggtca taaaacggcg ctgatttgtg aatccagcgg
    28801 cggagtcgtt aaccggtata gttatcttga gttaaatcag gagattaacc gcacggcaaa
    28861 cctgttttat acgctgggga ttcgcaaagg cgacaaggtt gcactacatc tcgacaactg
    28921 cccggaattt atcttttgct ggttcgggct ggcaaaaatt ggcgcgatta tggtgccgat
    28981 taacgcccgc ctgttacgcg aggaaagcgc gtggatcctg caaaatagcc aggcgtgcct
    29041 gctggtgacc agtgcgcaat tctatcctat gtatcaacag attcagcagg aagatgccac
    29101 tcaattgcgg cacatttgcc tgacagatgt ggcacttccc gctgatgatg gcgtgagttc
    29161 gtttactcaa ctgaaaaatc aacaacctgc caccttgtgc tatgcaccgc cgctattgac
    29221 tgacgatacg gcggaaattc tcttcacctc cggcaccacc tcccgaccga aaggtgtggt
    29281 gattacccat tacaacctgc gcttcgctgg atattactcc gcctggcagt gtgcactgcg
    29341 tgacgatgac gtctacctga cggtaatgcc tgcgtttcat atcgattgcc agtgtactgc
    29401 ggcgatggcg gcgttttctg ccggggccac ctttgtgctg gtcgagaaat acagcgcccg
    29461 cgccttctgg ggacaggtgc agaagtaccg cgccaccatt accgaatgta ttccgatgat
    29521 gatccgtacg ttgatggtgc agccgccttc agcgaacgat cggcaacacc gcctgcggga
    29581 agtgatgttt tatctcaact tgtcggagca ggaaaaagat gcgttttgtg aacgcttcgg
    29641 cgttcgcttg ctgacgtctt atgggatgac ggaaaccatt gtgggcatta tcggcgatcg
    29701 ccctggcgat aaacgacgct ggccgtcgat tggtcgggcg gggttttgct acgaagcgga
    29761 gatccgcgac gatcacaatc gcccgctccc ggcaggtgag atcggtgaaa tctgtattaa
    29821 aggcgtacca gggaaaacca tcttcaaaga gtattttctc aacccgaaag ccactgcgaa
    29881 agtgctggaa gccgacggct ggctacatac cggcgatacc ggatactgcg acgaagaggg
    29941 ctttttttat ttcgtcgatc gccgctgcaa tatgatcaaa cgcggtggcg agaatgtctc
    30001 ctgcgtggag ctggaaaata tcatcgccac ccatccaaaa attcaggata tcgtggttgt
    30061 gggtattaaa gattcgattc gcgatgaagc catcaaagca tttgtggtgc tgaatgaagg
    30121 tgaaacattg agcgaagagg aatttttccg cttctgcgaa caaaatatgg cgaaatttaa
    30181 agtgccctct tatctggaga tcagaaaaga tctgccacgt aattgctcgg ggaaaataat
    30241 tagaaagaat ctgaaataaa tgaaacagca gggaactacc ctgccagcta ataatcacgc
    30301 tataaaacaa tatgcatttt ttgcagggat gttgtcgtcc ctgaaaaagc aaaaatggag
    30361 aaaaggaatg agtgaatcat tacatctgac ccgcaatgga tcaattctgg aaattaccct
    30421 tgatcgtcca aaagcgaatg ctattgatgc aaaaaccagc tttgaaatgg gcgaagtatt
    30481 tctaaatttc cgtgacgatc cgcaattacg tgtcgccatt attaccggtg ccggagagaa
    30541 gttcttttcc gcgggctggg atttaaaagc ggcagcagaa ggcgaagcac cggatgctga
    30601 ctttggcccg ggtggatttg cgggattaac cgaaattttc aatctcgaca aaccggttat
    30661 cgcagctgtg aacggctatg cctttggcgg cggtttcgaa ctggcgctgg cggcagattt
    30721 tattgtttgt gccgataacg ccagcttcgc cctgccggaa gccaagctgg gcatcgttcc
    30781 tgacagcggc ggcgtgctgc gtctaccgaa gatcctgccg cctgccatcg tcaatgaaat
    30841 ggtgatgacc ggcagacgaa tgggcgcaga agaggcgctg cgttggggga tagtcaaccg
    30901 cgtggttagc caggcggaac tgatggataa cgcccgcgaa ctggcgcagc agctggttaa
    30961 cagcgccccg ctggcgattg cggcgctgaa agagatcttc cgcaccacca gcgaaatgcc
    31021 ggtagaagaa gcgtatcgct atattcgcag cggcgtgttg aaacactatc cgtcggttct
    31081 gcattcggag gatgccattg aagggccgct ggcgtttgcc gagaagcgcg atccggtgtg
    31141 gaaaggacgt taacaaccgt gagctattac gcctttgagg ggttaattcc ggtggttcac
    31201 ccgacggcgt ttgtccatcc cagtgccgtc ttgattggcg atgtgattgt gggagccggt
    31261 gtctacatcg gcccactcgc ctcactgcgt ggtgactacg ggcggttgat cgtgcaagcg
    31321 ggagccaata ttcaggatgg ctgcattatg catggctact gcgacactga caccatcgtt
    31381 ggggaaaacg gccatatcgg gcacggagcg atcctgcatg gttgtgtgat tggtcgcgat
    31441 gcattggtcg ggatgaacag cgtgattatg gatggcgcgg tcattggcga agagagcatt
    31501 gttgccgcca tgagctttgt caaagcaggc tttcacggcg agaaacgcca gttgttgatg
    31561 ggtacgccag cccgcgctgt acgcagtgtt agtgacgacg agttacactg gaaacggttg
    31621 aataccaaag agtatcagga tcttgtcggg cgctgtcatg catcgttaca tgaaacacag
    31681 ccactgaggc aaatggagga aaatcgcccc cgtttgcagg ggacgacgga tgtgacgcct
    31741 aaacggtgat cttgccggat gcgacctggt tgctggataa gatgctacaa catcgcatct
    31801 ggcaagcagg acgcccggta tcaacgagat aaaattaacg acgcatactc tttgacaaca
    31861 tcatctgcca cttctgctcg cggttaagtt ccgtgagcgg aacggcagga atacgcggcg
    31921 tctttcttac gccgactaat ttatcccggc tgttattgcg caggcgcgcg taaatttgct
    31981 catcgatatc gaccactttt accagtcgct ggcactggca tccccgtcct tccagcttat
    32041 tagggatcat cttaacttcg cagcttattt ctgcgacttc cgacagaata taagtcaggg
    32101 tattaaccgc cttgctgtgt tcaatatcga aatgaataga gatctctctt gctatcaccc
    32161 accgattttc agccatcatc cattcggcga ttaacaagta gagtggtttt tcaacatatc
    32221 cttcacacat acgaactcca gtttcacaaa ttaaaagtct ggctatgact cgttttgaag
    32281 caattaaaca aggccatatt aaaattgtgg atatatcgat agtatgcaac ttcaccgtcg
    32341 ataagtgtga gctaaatcct gcttatgtca tcaaaaatat cgattcccca aaagatttac
    32401 ttaatgggca aaaaaaacgg tcctcatcag agaaccgtat tacttattcg atcaaattag
    32461 cggatgaaaa atatccgcca tgacacgcta ttatttgatc tgcgcgtgca tttcctgcac
    32521 cgaaattact ttttcggtcg cgtcggcatt cagcgccatc gcggtagcga aaccaccgtt
    32581 cagggtggtg tcataatgca ctttatattg cagcgcactg cgacggatca cgcgggagtc
    32641 ttcaatcgcg cgacggcctg aagtggtgtt gatgatgtag gtatattcgc cattcttgat
    32701 gcggtcctga atgtgcggac ggccttcatg caccttgttc accagacgcg gattaatgcc
    32761 cgcttcgccc agcacaatcg ccgtgccgtg ggtcgcatcc agctcgaagc cctgtttcag
    32821 cagttttgcc gccaggtcca ccacgcgttc tttatcgcct tcgcgcacgg aaagcaacgc
    32881 acgaccgtgt ttcttcatgg tggagttgct gcccagctgc gctttggcaa acgcttcagc
    32941 gaaggtgcgg cccacgccca tcacttcccc ggtggagcgc atttctggcc ctaacagcgg
    33001 gtcaacaccc gggaatttat tgaacggcag caccacttct ttcaccgagt agtacggcgg
    33061 gataacttct ttggttacgc cctgctcagc cagcgatttg ccagccatca cacgcgccgc
    33121 cacttttgcc agcggtacgc cggtggcttt ggagacgaac ggaacggtac gcgccgcacg
    33181 cgggttaact tcaatcaggt agacttcgtt gtttttcacc gcaaactgca cgttcatcag
    33241 accgcgcacc tgcaattcga aggccagttt ctgcacctgc tggcgcatca catcctgaat
    33301 ttcctgactt aaggtgtagg ctggcagaga acatgcggag tcaccggagt gcacgcccgc
    33361 ctgctcgatg tgctccatga tgccgccaat cagcaccatt tcgccgtcgc agatggcatc
    33421 cacgtcaact tctaccgcgt catcaaggaa gtgatccagc aacactggcg catcgttaga
    33481 cacgctgacc gccgtctgga agtagcgacg caggtcagct tcgtcataga cgatttccat
    33541 cgcccgaccg ccgagaacgt aagacggacg taccaccagc gggtagccaa tctctttcgc
    33601 cttctctacc gccatttcaa tagcggtaac ggtggcgttc gccggttgtt tcagtttcag
    33661 acgctcaacc gcatgctgga agcgttcacg gtcttctgca cggtcgatag catccgggct
    33721 ggtgccgata accggtacgc cagcagcttc cagcgcgcgc gccagtttca gcggggtctg
    33781 accgccgtac tgaacgataa cgcctttcgg cttctcaata cgcacgattt ctagcacatc
    33841 ttccagtgtt accggctcga agtagaggcg atcggaagtg tcgtagtcgg tggagacggt
    33901 ttccgggtta cagttaacca taatggtttc gtaaccgtct tcgcgcagtg ccagcgaggc
    33961 gtgtacacag cagtagtcga attcgatacc ctgaccgata cggttcgggc cgccgccgag
    34021 aaccatgatt ttttcacggt cggtagacgg attcgcttcg cactcttctt cataagtgga
    34081 gtacatgtaa gcggtgtcgg tggcgaactc tgccgcacag gtatccacgc gcttataaac
    34141 cgggtgcagg tcatactggt cgcgcagctt gcggatttcc gcttcgcgca cgcccgccag
    34201 ttttgccaga cgcgcatcgg caaagccttt gcgtttcagc tggcgcagga attcagcgtt
    34261 caggccagtg atgcccactt ccgctacttt ctcttccaga cgcaccagtt cttcaatctg
    34321 taccaggaac cagcggtcaa tattggtcag gttgaacacg ccgtccacag acaggcccgc
    34381 acggaaggca tcggcgatgt accagatacg ctctgcgcca gcatctttca gttcgcgacg
    34441 gattttggtt aacgcttccg ggtcatcgag gctcactttc gggtcgaatc cagtcgcacc
    34501 gacttccagg ccgcgcagcg ctttttgcag ggattcctgc tgcgtgcgac caatcgccat
    34561 cacttcgcca accgatttca tctgagtggt cagacggtcg ttagcaccag cgaatttttc
    34621 gaagttgaag cgaggaattt tagtaaccac atagtcgatg gacggctcga aggaggccgg
    34681 agtacgtccg ccagtgatgt cgttcatcag ttcgtcgagg gtgtaaccca ccgccagttt
    34741 cgccgccact ttagcaatcg ggaaaccggt cgctttcgac gccagcgccg aagaacggga
    34801 cacgcgcggg ttcatttcga taacaatcag gcgaccgttt ttcgggttca ccgcaaactg
    34861 gacattggaa ccaccggttt caacgccgat ttcacgcagc accgccatcg aggcgttacg
    34921 catgatttga tattctttgt cggtcagcgt ttgggctggc gcgacagtga tggagtcacc
    34981 ggtatggatg cccatcgcat caaagttttc gatagagcag acgatgatgc agttgtcgtt
    35041 tttatcacgc accacttcca tctcgtactc tttccagcca atcagcgact catcaatcag
    35101 caactctttg gtcggagaga gatccagacc gcgggcgcaa atttcttcaa actcttcgcg
    35161 gttataagcg ataccgccgc cgctaccgcc catggtaaag gatgggcgaa taatgcacgg
    35221 gaagcccacg tcagcggcaa ccgccagcgc ttcttccatc gtgtgtgcga taccggaacg
    35281 cgcggtttcc agaccaattt tcttcatcgc tacgtcgaaa cgacggcggt cttctgcttt
    35341 atcaatcgca tcggcggtcg caccaatcat agtcacgccg aactcttcca acacgccctg
    35401 acgctccagc tccagcgcgc agttcagcgc cgtctgaccg cccatcgttg gcagcaccgc
    35461 gtccgggcgc tctttttcaa taatcttgcg taccacttcc cagtgaatcg gctcgatgta
    35521 ggtcgcatcg gccatttccg ggtcggtcat gatggtcgcc gggttggagt tcaccagaat
    35581 gacccggtaa ccctcttcgc gcagggcttt acatgcttgc gcgccagagt agtcaaactc
    35641 acacgcctga ccgataacaa tcgggcccgc acccagaatc aggatacttt ttatatctgt
    35701 acgttttggc atggctcttt tactcctgat tacttagcgg ttttacggta ctgctcaatt
    35761 aactcgataa agtggtcgaa caacggcgcg gcgtcgtgtg gaccagggct ggcttcaggg
    35821 tgcccctgga agctgaacgc cggtttatcg gtgcgatgaa tgccctgtaa cgtaccgtcg
    35881 aacagggatt tatgcgtgac acgcaggttt gctggtaatg ttgcttcgtc caccgcaaaa
    35941 ccgtggttct gggcggtgat catcaccacg tttttctcaa catctttaac cggatggttg
    36001 ccgccgtggt gaccaaattt cattttgaca gtcttcgcac cgctcgccag cgccagcagc
    36061 tgatgaccga ggcagatgcc gaagaccgga atatcggttt cgaggaattt ctggatggcg
    36121 gtaatggcgt aatcgcacgg ggccgggtcg ccaggaccgt tggagaggaa gataccgtct
    36181 ggattcattt tcagcacatc ttccgcagaa gtttgcgccg gaacgatggt caggcgacag
    36241 cctctgtcca ccaacatgcg caggatgttg cgcttggcac caaaatcata agccacgacg
    36301 tggaacggca gctcgtcttc ttttttcgct tctggcaggc cgccggtcaa cgtccagctc
    36361 ccttgtgtcc agctataggc ttctgcggtg gtcacttctt ttgccagatc catgccattc
    36421 agacctggga acgcgcgggc tttttctaac gccaacgccg catccgggtt atcgcctgcg
    36481 ataatgcagc cattctgtgc gcctttctcg cgcagtaaac gcgtcagctt acgggtatcg
    36541 atatcggcaa tcgccacgat gttatggcgc ttcaggtaag aagagaggtc ttcggtatta
    36601 cggaagttgc tggcaatcag cggcaggtcg cgaatcacca gaccttgtgc atgtacctga
    36661 gaagattctt catcggcgtc attagtgccg acattgccaa tatggggata agtaagagta
    36721 acgatttgac gagaatagga aggatcagtg aggatttctt gataaccggt cattgaagta
    36781 ttgaaaacga cttccccaac cgccgaacct gttgccccta tggcccgacc gtgaaactgg
    36841 gttccgtctt ccagaaccaa tagcgctgac ttaatcaaaa caccctccag agaatattca
    36901 ctcactttat ttgcatatta attcacaatg atgacgtaaa tcaatgcaaa tctgcttacc
    36961 gacgaatttc tggcaaacgg cggcattctg gagatatggg cgctaaaagt caacttaatg
    37021 gcgggatttt ttatattatt ttgtggctca tgcatgtttt acaggcatta agcggcaaaa
    37081 agatcatata acctgttctg gaaaacgctt gcgcagcaag aagattgcat gaaataaaag
    37141 cgagtaggga aaagtggacc aaatggtcaa aatttacatt aaaacaacaa aaaccgaaga
    37201 caaaacatgt aatttttggc acttatagac aatttaaaaa cataacacta aaaataaaag
    37261 ggccaaaaat taaagccctt tacaatcaat aaattaaatg tgttattttt gcaccataac
    37321 aaatattttg tggttacaaa ctattgagat caagcacatc tcgcatatca aaaagaccac
    37381 tttccttacc actcaaccac aaagccgatc ttaccgcgcc gttagcaaat gtcatgcggc
    37441 tggacgcctt atgggtgatc tccagacgct cgccaatatc ggcaaacatc gcggtatgtt
    37501 cgccaacgat gtcacctgca cgcacggtgg caaaaccaat ggtgccaggc acacgttcac
    37561 cggtgtggcc ttcacgactg tagaccgcgc aatctttcag atctttatca agggcgtggg
    37621 cgatcgcctc tcccattgcc agtgcggtgc ctgacggcgc atcaacttta tgtctatgat
    37681 gtgcttcaat aatttcgata tcggtgtagt cacccatcac tttggctgct ttctccagca
    37741 gcttaagcat gacgttaacg ccaacgctaa aattggcggc aaagacaatc gcaatatcgg
    37801 cggcggcgtc acgaatcgcc tgtttaccgg cttcgtcaaa ccccgtagtg ccgatcacca
    37861 tccctttgcc atgctggcga caaaaagcga gatggttcag cgtaccttcc ggacgggtaa
    37921 aatcgataaa cacatcaaaa tcatctttta ccgcatcgag gctgctttgc acggtaacgc
    37981 ctgttttccc tgcaccagcc agctcaccgg cgtcgctgcc cagtaaagaa gatccttcgc
    38041 gctccagcgc cgcgcccagc tgcacgcctt ctaatgccag cgccgcctga atcaactggc
    38101 ggcccatacg ccccccggct cccgcgatgg caacgcggat gtttgcatca tgcatagcta
    38161 ttctcttttg ttaatttgca tagaccgttt tcagagtaac cagcgcatcg ctacgccgcc
    38221 agccaaaaac gctgataatt agaaattaat gataaacagg gagggatatc agtgaaaggc
    38281 atgagtcgat gaatgactgc atgccaataa catgtgacag gttacgacgc cagagccagc
    38341 acctcagcca cccactgctg gaagcctttc acatccagat ccaatgccac ctgtacattg
    38401 gctggcttgc ccaggcaacc gtcgatatca accaccgtcg tgcctgaggt aaattcgccc
    38461 tgagtttcca ctgccacaaa acagggtttg agagtgaaca ggtccgggcg caccagccag
    38521 gcgatggcgc agagatcgtg cattcgcaag ccgctttgca tactgccgct acggtagtgg
    38581 ctaaacaggg cgtgaagcat tttcccggta cggtttaact gcggcagtgt agagagatag
    38641 tcaggagtta atattgcctg attggtgaca tccaaaccgc acatgacgat ttcaatacca
    38701 ctgcggaaga cacaggcagc agcttctgga tcggcagcaa tattaaactc ggcgtttggc
    38761 gtacagttgc cgcgtccggc agaaccaccc atgatcacca gacggcgaat atacggcttg
    38821 cattccgggc attgtgaaag taacagcgca atattggtta acgggccgat ggccaccagg
    38881 gtaacaggct ctggtgcacg catcagggca tcccgaatcg ccagaaacgc cggtatcccg
    38941 agcggctttc ggttgtgctc aacaaagtcg tagccagcca ttcccgattc gccgtgcaca
    39001 gatgccgcat cacgcggtgc gcgtaccagc ggcacagcgg ccccttgggc gagcggaatc
    39061 tccgcattcc agaaatgcag cagttgcagg gcattgcggg tagttttctc aaccgagaca
    39121 ttacccgcga cggtggtcat cagttgcagg tcgagttcgg gtgcaaaaat cgcggcggca
    39181 atggcgacgg catcgtcaat gccggggtca gtatcgagga agataggtaa acgcatgttt
    39241 tctccataaa aaatgccggt aacaagaccg gcattttcgc ataacttagg ctgctaatga
    39301 cttaatcgac ttcacgaata tcgacacgca gctctttcgg cacttcgaaa acaatgtttt
    39361 cttcacggcc ttccagcgga atggcttcac caccgcccag ctgctgcaaa cgtgccacca
    39421 cattctgcac cagaatatcc ggagccgatg cgcccgcagt cacgccgacg catttaacct
    39481 ctttcaccca ctcttcctgg atatctttcg catcgtcaat caaaaacgcg cgtttgccca
    39541 tacgctgggc cagctccgcc agacggttgg agttggagga gtttttcgaa ccgaccacca
    39601 acacaacttc cgcctgttct gccagggcgc gtaccgcttc ctgacggtta gtcgtggcgt
    39661 agcagatgtc atctttgcgc ggaccgacaa ttttcgggaa gcgtttacgc agcgcgtcga
    39721 tcacatcaga cgtgtcatcc accgacagcg tggtctgggt cataaaggag agcttctctt
    39781 cgtttttgac cgtcagtttc cacacatcgt ccggcgattc gaccagatac attccccctt
    39841 ccgggttact gtactggccc attgtccctt ccacttccgg gtgcccggcg tgaccgatga
    39901 gaatagattc ttcgccacgg cgactggcgc gggcgacttc catatgcact ttggtcacca
    39961 gcggacaggt cgcgtcaaat accgtcaaat cgcgactttt tgcttcgtta cgtaccgcct
    40021 gagaaacacc gtgtgcggag aaaatcagga tcgcgccgtc cggtacttcg ctaatctgct
    40081 caataaagat agccccacgc tcacgcaagc tatcgaccac atagcggtta tgtaccactt
    40141 cgtgacggac atatatcggt gcgccgtaaa tggccagcgc gttttcaaca atgctgatag
    40201 cgcggtctac cccggcacaa aaaccacgcg ggttggccaa caggatctgc atgttacgcc
    40261 tccagtgccg gatcgatttc cagcacttca atatcaaaat gaacggtctg cccggccagc
    40321 ggatggttga aatcaacggt aatggagtcg ccgttaattt cgcggatcac gccaggcatc
    40381 tcactgccat ccattgcggt aaaaagcata attgcgccaa tttctggctc gcctgcatcc
    40441 ataaattcac ggcgggagaa gtactgaatc aggtccggtg acggcacgcc aaacgccgca
    40501 tcgggctcca gcgagaaggt ggttttatcg cccactttca gccccaacag gtgttgctcc
    40561 agcccttcag aaagagaagc atcgcccagg cggaacagcg ccggtttacc gttgttgcgg
    40621 gtagactcag cggtggtgcc atcatcgagt ttcagcgtga agtgcaccag gacggcgcta
    40681 ttgctctgta cagattcaga catgcaggtt tgctcttata ttttgaggat ttttgccgga
    40741 tgcggcctac gatttcgcag caatctgtag gccttatccg gcagggttta ttattgtttt
    40801 ttcgctttag aaggcaaaaa accttccagc acaatcagtg ccgcaccgac acaaatggca
    40861 gtatcggcaa ggttgaaggt ggcgaagtgc cagtcgccga cgtagaagtc gatcatatcg
    40921 acaacgaagc cgtgccacag gcggtcgaac aggttgccca gcgcgccgcc aataatcagc
    40981 gcataagcga tattgtttag cttctgcgtg gccttcgagc gatacatcat cactgccagg
    41041 atcacgctaa taccaatcgc aataccggca aagaaccaac gctgccagcc gccgctatcg
    41101 gcaaggaaac taaacgccgc gccatagtta cgcgcataat gcagattaag cgacgggaac
    41161 agcgggaccg tatcccccag agcaaagttc tggaggatca ggtatttgct gcccagatcg
    41221 ataatcagca cgactaccac cagccacagc cagcgtagcc ctgttgaaca gatcgattga
    41281 ctcatcaggc aaacttacgt ttttcaccgt caccggcgac gttgctgaca cagcggccgc
    41341 agatttctgc gtgttccgcc accttgccga catcctgggt gtagtgccag cagcgtgggc
    41401 acttctcacc ttcggcttta ctcaacgcga ctttcagccc tttgagtact tcgctctgct
    41461 gagcatcagc aggtgcgtca ttatagtctg caacggtagc gccggaggtc aacaggacaa
    41521 atcgtaattc atcgcccagc gcggtcagtt tcgccgccag ttccggttct gcatacaagg
    41581 ttactgccgc ttccagcgag ccacccactt tcttatcggc acgcgcttgc tcaatgactt
    41641 tgttcacttc gccacgcact ttcaacagct cgtcccagaa cgcatcgttc atcgcttcac
    41701 tgtctgccag accaaacagg ccttcgtacc actcaccggt aaagacgtat ttttcacgtt
    41761 cgcccggcag atagccccac acttcatcag cggtgaagga gaggattggt gccatccagc
    41821 gcaccagcgc ttcagcgatg tgatacagcg cagtctggca gctacgacgc gccacgctgt
    41881 ccgctttggc ggtgtactga cggtctttga tgatgtcgag gtagaaggaa cccatctcaa
    41941 cggagcagaa gcgcatcaga cgctgtacca cttcgtggaa atcgtatgct tcgtacgcct
    42001 tgaggatgtc ttcctgtgcc gctttcgcac aacctacggc ccagcgatcc agtaccacca
    42061 tctcttccgg tttcaccata tcttttgctg gatcaaaacc gttcaggttt gccagcagga
    42121 agcgcgcggt gttacggata cgacgatagc tatcggcagc acgtttcagg atctcgtcag
    42181 aaacggccat ttcaccggtg tagtcggttg atgccaccca cagacgcaga atatccgcgc
    42241 ccagtttgtt catcacatcc tgcggcgaaa cggtattgcc gatggattta gacatcttgc
    42301 gaccctgacc atccacggta aagccgtggg tcagtacctg acgatacggc gctttgcctt
    42361 tcatcgcggt ggagatcatt agggaagaca tgaaccaacc acggtgctgg tcagaacctt
    42421 ccagatacat gtccgctgcg tgaccggcaa attccggacg cacgtcaaca acagaagagt
    42481 gggtagatcc ggagtcaaac catacatcca atgtgtccgg cactttcacg tactgatcag
    42541 cttcatcgcc gaggatctct ttcgcatcaa gatcccacca cgcctggatg ccgtcaactt
    42601 caacgcgttt tgccacttct tccattagtt cgagagtacg cggatgcagc tcttccgtgt
    42661 ctttgtgcac gaacagtgac atcggtacgc cccaggtgcg ctgacgggag atacaccagt
    42721 caggacggtt agcaaccatc gactcgatac gcgcctggcc ccagtccggg atccactgca
    42781 cgcctttgat ctctttcagt gactgcgcac gcagaccttt ctgatccatg ctgacgaacc
    42841 actgcggcgt cgcgcggaag atgatcggcg ttttgtgacg ccagcagcac ggatagctgt
    42901 gctgcatttt ctcaacgtgc agcagcgcgc ctttttcctg cagcagcgca acgacgatgt
    42961 cgttcgcttt gaagacgttc acgccatcca gcgtcggata agtgcccggc agataagtgc
    43021 cgtccgggcc aaccgggtta gcggtttcca ggccgtattt ctgaccgatc acatagtcgt
    43081 ccgggccgtg gccaggcgcg gtgtgaacgg caccggtacc ggcatccagg gtaacgtgat
    43141 cgccgaggat tgccggaacg tcgaagccca taaacggatg ggtaaagcgc agcaactcaa
    43201 gctccgcacc ttttaccgtg ccgagaatgg tgtaatcggt cacgccgata cgctgcatta
    43261 cgctttcaac cagatctttc gccagaatca cggcctgacc gtcgatctgc accagcgcat
    43321 agtcgaaatc aggtgcaata gagattgcgc ggttcgcagg cagagtccac ggcgtagtgg
    43381 tccagatcac cagcgagatt gggccgttaa cgttgcttac ggcaaatttt gctttcagtg
    43441 catcctgatc gaccgcctga aaagcaacgt cgatggacgg agaagttttg tcgtaatact
    43501 caacttccgc ttccgccagc gcagaacggc agtcaacgca ccagtgaact ggcttcgcgc
    43561 ctttgtgcag gtgaccgttg ccgatgattt tgcccagcgc gcggatgatg ttggcttcag
    43621 ttttgaagtc catggtcagg tacgggtgcg accagtcgcc cagcacgccc agacggataa
    43681 agtctttgcg ttgaccgtca acctgggtcg ccgcgtattc gcggcacttg gcgcggaact
    43741 cggcggcggt gaatttctca cccggcttac cgtattcttg ctctactttc agctcgatcg
    43801 gcagaccgtg gcagtcccag ccaggcacat acggcgagtc ataaccggaa agccctttgg
    43861 acttcacgat aatgtctttc agaatcttgt taaccgagtg accaatatga atgctgccat
    43921 tcgcataagg agggccatca tgcagaatga aggttttttt gcctttttta gccgcacgga
    43981 tgatgccgta cagatcatca tcagtccaac gcgccagcat tcccggttcg cgcttggcga
    44041 gatcgccacg catcgggaac cctgtttccg gcaaattcag ggttgattta tagtcactca
    44101 tcagattctc ggttccgtgt ttcggtttga ttacataaca ggcttaagcc ggttttgtta
    44161 gcccaaaaaa ttcgcgggcg gttaattcat cacgcgcaat ctgcgctttc agttcgtcca
    44221 gcgacgcaaa tcgctgctca ttgcgtattt ttttacgcag cactacttgt atatggcgac
    44281 cgtaaaggtc cattgcaaca cctaacaaat gcacttccag ctgctggcga ataccggcaa
    44341 ccgttgggcg tgttccaatg tttgccacgc cgggtaacgg cttttcaccg aggcccagca
    44401 cttctaccgc ataaacccct ttcaccgggg aaacctgacg gcgtagcggt acattcgccg
    44461 tcgggaaacc tatagtgcgc cctaattcat caccgtggac tacacgcccg gagatggcaa
    44521 acgggtgccc cagtaaactt tctgccagag ccagattgtc atccgccagc gcctgacgca
    44581 cagcggtgct gctgatacgc acgccacctt cgcaaaaagt ttgcgtactg gtgatatcga
    44641 agccgtattc catgccagct ttctgtaata acaagaaatc gccttcacga ccagcgccaa
    44701 agcggaaatc atcacctacg gcaagaaatt ttacgcgcaa atgcttcacc agaagatcgc
    44761 tgatgaaatt ttgcgcggtt aacgccgcga aacgcctgtc gaaacgcacg cacagcacgt
    44821 aatcaacgcc acactctgca aggtaacgca gtttttcccg cagccgggtc agccgtgccg
    44881 gggctttatc ggtagcaaac agttccagtg gttgaggttc aaaaagcatc accatcaccg
    44941 gtaagttgcg cttgcgccct tcttcctgca agccctgtaa cagcgcgcga tgaccgcgat
    45001 gcacgccgtc gaaattacca atagtcagca cacacccttc ttgcggggcc tggctgagat
    45061 tatgtatgcc gcgtatcagc ttcatgtctg gctcaaaaca gtgaaaatcg tccgagtata
    45121 ccttgtacag cggtcaaggt taaccggcga ttgagtaccg aaatcagaaa gaagtggctt
    45181 ttcattgcca tggcgcaaat cacgggaaga aagtgaccgc ctgctgcaat ttttatcgcg
    45241 gaaaagctgt attcacaccc tgcaagctgg tagaatcctg cgccatcact acgtaacgag
    45301 tgccggcaca ttaacggcgc ttatttgcac aaatccattg acaaaagaag gctaaaaggg
    45361 catattcctc ggcctttgaa ttgtccatat agaacacatt tgggagttgg accttggcta
    45421 atatcaaatc agctaagaag cgcgccattc agtctgaaaa ggctcgtaag cacaacgcaa
    45481 gccgtcgctc tatgatgcgt actttcatca agaaagtata cgcagctatc gaagctggcg
    45541 acaaagctgc tgcacagaaa gcatttaacg aaatgcaacc gatcgtggac cgtcaggctg
    45601 ctaaaggtct gatccacaaa aacaaagctg cacgtcataa ggctaacctg actgcacaga
    45661 tcaacaaact ggcttaatcg ccaatttgct gaagctttgt gaaaaagccc gcgcaagcgg
    45721 gtttttttat gcctgctgct tttatgatgc gtgagagaac acactaccgg ttgccagata
    45781 gtcaaaagat gctggctttt gcagacatgc cgttacactt tctgaactct tcgtggctta
    45841 agaatcgtag aaccatcggc tgatacattc tgaacaagaa tatctactca atctccagac
    45901 tcccctacaa agaatgtgga tattgtgata cacat
//
LOCUS       DAZGLJ010000011        45772 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000011 DAZGLJ010000000
VERSION     DAZGLJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.011"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            238..864
                     /locus_tag="ACHK49_000566"
     CDS             238..864
                     /locus_tag="ACHK49_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO9261261.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            885..2987
                     /locus_tag="ACHK49_000567"
     CDS             885..2987
                     /locus_tag="ACHK49_000567"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO9261262.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            3000..3638
                     /locus_tag="ACHK49_000568"
     CDS             3000..3638
                     /locus_tag="ACHK49_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO9261263.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3702..4370
                     /locus_tag="ACHK49_000569"
     CDS             3702..4370
                     /locus_tag="ACHK49_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO9261264.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4367..5152
                     /gene="phsC"
                     /locus_tag="ACHK49_000570"
     CDS             4367..5152
                     /gene="phsC"
                     /locus_tag="ACHK49_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO9261265.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5156..5968
                     /gene="ydhT"
                     /locus_tag="ACHK49_000571"
     CDS             5156..5968
                     /gene="ydhT"
                     /locus_tag="ACHK49_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO9261266.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(5980..7584)
                     /locus_tag="ACHK49_000572"
     CDS             complement(5980..7584)
                     /locus_tag="ACHK49_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO9261267.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDIGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7710..8015)
                     /locus_tag="ACHK49_000573"
     CDS             complement(7710..8015)
                     /locus_tag="ACHK49_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO9261268.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8123..8199)
                     /locus_tag="ACHK49_000574"
     tRNA            complement(8123..8199)
                     /locus_tag="ACHK49_000574"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8163..8165),aa:Val,seq:gac)
     gene            complement(8204..8280)
                     /locus_tag="ACHK49_000575"
     tRNA            complement(8204..8280)
                     /locus_tag="ACHK49_000575"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8244..8246),aa:Val,seq:gac)
     gene            8588..9844
                     /locus_tag="ACHK49_000576"
     CDS             8588..9844
                     /locus_tag="ACHK49_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO9261269.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEERA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9885..11258)
                     /gene="mdtK"
                     /locus_tag="ACHK49_000577"
     CDS             complement(9885..11258)
                     /gene="mdtK"
                     /locus_tag="ACHK49_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO9261270.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            11473..12114
                     /gene="ribE"
                     /locus_tag="ACHK49_000578"
     CDS             11473..12114
                     /gene="ribE"
                     /locus_tag="ACHK49_000578"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO9261271.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12154..13302)
                     /gene="cfa"
                     /locus_tag="ACHK49_000579"
     CDS             complement(12154..13302)
                     /gene="cfa"
                     /locus_tag="ACHK49_000579"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO9261272.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAMVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13593..14804)
                     /gene="punC"
                     /locus_tag="ACHK49_000580"
     CDS             complement(13593..14804)
                     /gene="punC"
                     /locus_tag="ACHK49_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO9261273.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14917..15849
                     /gene="punR"
                     /locus_tag="ACHK49_000581"
     CDS             14917..15849
                     /gene="punR"
                     /locus_tag="ACHK49_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO9261274.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15846..16871)
                     /gene="purR"
                     /locus_tag="ACHK49_000582"
     CDS             complement(15846..16871)
                     /gene="purR"
                     /locus_tag="ACHK49_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO9261275.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17170..17259
                     /gene="cydH"
                     /locus_tag="ACHK49_000583"
                     /gene_synonym="ynhF"
     CDS             17170..17259
                     /gene="cydH"
                     /locus_tag="ACHK49_000583"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO9261276.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17425..18594
                     /locus_tag="ACHK49_000584"
     CDS             17425..18594
                     /locus_tag="ACHK49_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9261277.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18740..19321)
                     /gene="sodB"
                     /locus_tag="ACHK49_000585"
     CDS             complement(18740..19321)
                     /gene="sodB"
                     /locus_tag="ACHK49_000585"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO9261278.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19449..20276)
                     /gene="mepH"
                     /locus_tag="ACHK49_000586"
     CDS             complement(19449..20276)
                     /gene="mepH"
                     /locus_tag="ACHK49_000586"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO9261279.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20611..20958
                     /gene="grxD"
                     /locus_tag="ACHK49_000587"
     CDS             20611..20958
                     /gene="grxD"
                     /locus_tag="ACHK49_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO9261280.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21009..25625)
                     /locus_tag="ACHK49_000588"
     CDS             complement(21009..25625)
                     /locus_tag="ACHK49_000588"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO9261281.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQECSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDTAHFE
                     STSGATSNHVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVSDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECTARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIIAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25718..26365)
                     /gene="rnt"
                     /locus_tag="ACHK49_000589"
     CDS             complement(25718..26365)
                     /gene="rnt"
                     /locus_tag="ACHK49_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO9261282.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26468..26875)
                     /gene="gloA"
                     /locus_tag="ACHK49_000590"
     CDS             complement(26468..26875)
                     /gene="gloA"
                     /locus_tag="ACHK49_000590"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO9261283.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26956..28053)
                     /gene="nemA"
                     /locus_tag="ACHK49_000591"
     CDS             complement(26956..28053)
                     /gene="nemA"
                     /locus_tag="ACHK49_000591"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO9261284.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGANEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28090..28689)
                     /gene="nemR"
                     /locus_tag="ACHK49_000592"
     CDS             complement(28090..28689)
                     /gene="nemR"
                     /locus_tag="ACHK49_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO9261285.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            28792..29031
                     /locus_tag="ACHK49_000593"
     CDS             28792..29031
                     /locus_tag="ACHK49_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO9261286.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29080..29976
                     /locus_tag="ACHK49_000594"
     CDS             29080..29976
                     /locus_tag="ACHK49_000594"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO9261287.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQQLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            30057..30578
                     /gene="sodC"
                     /locus_tag="ACHK49_000595"
     CDS             30057..30578
                     /gene="sodC"
                     /locus_tag="ACHK49_000595"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO9261288.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30579..32591)
                     /locus_tag="ACHK49_000596"
                     /pseudo
     CDS             complement(30579..32591)
                     /locus_tag="ACHK49_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(32591..33448)
                     /locus_tag="ACHK49_000597"
     CDS             complement(32591..33448)
                     /locus_tag="ACHK49_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO9261289.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33451..33687)
                     /locus_tag="ACHK49_000598"
     CDS             complement(33451..33687)
                     /locus_tag="ACHK49_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO9261290.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33888..34322
                     /gene="slyA"
                     /locus_tag="ACHK49_000599"
     CDS             33888..34322
                     /gene="slyA"
                     /locus_tag="ACHK49_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO9261291.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34369..34836)
                     /gene="slyB"
                     /locus_tag="ACHK49_000600"
     CDS             complement(34369..34836)
                     /gene="slyB"
                     /locus_tag="ACHK49_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO9261292.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35110..36219
                     /gene="anmK"
                     /locus_tag="ACHK49_000601"
     CDS             35110..36219
                     /gene="anmK"
                     /locus_tag="ACHK49_000601"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO9261293.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVEPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36317..36646
                     /gene="mliC"
                     /locus_tag="ACHK49_000602"
     CDS             36317..36646
                     /gene="mliC"
                     /locus_tag="ACHK49_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO9261294.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36705..37361
                     /gene="pdxH"
                     /locus_tag="ACHK49_000603"
     CDS             36705..37361
                     /gene="pdxH"
                     /locus_tag="ACHK49_000603"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO9261295.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37490..38764
                     /gene="tyrS"
                     /locus_tag="ACHK49_000604"
     CDS             37490..38764
                     /gene="tyrS"
                     /locus_tag="ACHK49_000604"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO9261296.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38823..39686
                     /gene="pdxY"
                     /locus_tag="ACHK49_000605"
     CDS             38823..39686
                     /gene="pdxY"
                     /locus_tag="ACHK49_000605"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO9261297.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39730..40335)
                     /gene="gstA"
                     /locus_tag="ACHK49_000606"
     CDS             complement(39730..40335)
                     /gene="gstA"
                     /locus_tag="ACHK49_000606"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO9261298.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40441..41943)
                     /gene="dtpA"
                     /locus_tag="ACHK49_000607"
     CDS             complement(40441..41943)
                     /gene="dtpA"
                     /locus_tag="ACHK49_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO9261299.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42554..43189)
                     /gene="nth"
                     /locus_tag="ACHK49_000608"
     CDS             complement(42554..43189)
                     /gene="nth"
                     /locus_tag="ACHK49_000608"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO9261300.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     LVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43189..43884)
                     /gene="rsxE"
                     /locus_tag="ACHK49_000609"
     CDS             complement(43189..43884)
                     /gene="rsxE"
                     /locus_tag="ACHK49_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO9261301.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(43888..44508)
                     /gene="rsxG"
                     /locus_tag="ACHK49_000610"
     CDS             complement(43888..44508)
                     /gene="rsxG"
                     /locus_tag="ACHK49_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO9261302.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44512..45570)
                     /gene="rsxD"
                     /locus_tag="ACHK49_000611"
     CDS             complement(44512..45570)
                     /gene="rsxD"
                     /locus_tag="ACHK49_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO9261303.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45571..>45772)
                     /locus_tag="ACHK49_000612"
     CDS             complement(45571..>45772)
                     /locus_tag="ACHK49_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9261304.1"
                     /translation="EPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAA
                     VAAAIARAQAKKAAQQKVVNED"
BASE COUNT        11532 a        11315 c        11929 g        10996 t
ORIGIN      
        1 atttcttgct ttgtaatttt aattgataac gatcaatgtt aagggctcag aataatcaca
       61 ttattttttc tcctaaccat aaaggattag tttatcggcg ttaaatcttt tctcatacac
      121 tatcgggtta gggaaaatat ctggtgttgg ggacagagtc agcagcgtat tagttgaatg
      181 cttaatatca ggctgctgaa ttgtcagcac tgtgtttgcg aagaggatgc gaaataaatg
      241 aacccggttg atcgtccact attagatatt ggcttaacgc gattagaatt cttgcgtata
      301 tcaggaaaag gccttgctgg tctaacaatt gcccctgcgt tactgtcact tttgggttgc
      361 aaacaggaag atattgatag cggcacagta gggttgataa atacacccaa aggggtgctg
      421 gtgacccagc gagcgcgatg cacgggttgc caccgctgtg aaatctcttg taccaacttc
      481 aacgatggct cagtagggac attcttctcc cgtatcaaaa tccaccgcaa ttatttcttt
      541 ggcgacaacg gggttggctc tggcggcggc ctgtatggcg atctcaacta taccgcggac
      601 acctgccgtc aatgcaaaga gccgcaatgc atgaacgtct gcccgattgg tgcgattacc
      661 tggcagcaga aagaaggctg tattaccgtc gatcataaac gctgtattgg ctgtagcgcc
      721 tgtactacgg catgcccgtg gatgatggcc accgtaaata ccgaaagtaa aaaatcctcg
      781 aaatgtgtgt tatgcggtga atgcgcaaac gcctgcccaa caggggcgtt aaaaattatc
      841 gagtggaaag atattactgt gtgaatcttg caaaggaaaa aatcatggct aacggttgga
      901 caggtaatat attaagagtc aatctcacga caggaaatat taccctcgaa gattccagta
      961 agtttaaaag ttttgtcggt ggcatgggct tcggctacaa aattatgtat gacgaagtac
     1021 cgccaggcac gaaacctttc gatgaagcga ataaattagt ctttgctacc ggcccattaa
     1081 ctggatctgg tgccccctgt agttctcgcg taaatatcac ctcactttct acttttacca
     1141 aaggaaattt agttgtcgat gcccatatgg gtggcttttt tgcagcgcaa atgaaattcg
     1201 ctggatacga cgtcattatt atcgaaggga aggcgaaatc accggtatgg ctgaagatta
     1261 aagatgacaa agttagcctg gaaaaagccg atttcttatg gggaaaaggg acgcgcgcaa
     1321 cgacggaaga aatttgtcga ttgaccagtc cggaaacctg tgtggcggct attggtcagg
     1381 ctggggaaaa ccttgttcct ctctctggca tgttgaatag ccgtaaccac agcggcggtg
     1441 cgggaactgg cgcaattatg ggttcgaaaa acctgaaagc gattgcggtt gaagggacga
     1501 aaggggtcaa cattgccgat cgtcaggaga tgaagcgtct caacgattac atgatgactg
     1561 aacttattgg tgcgaataac aaccatgtcg tgccaagtac gccgcaatcg tgggcagagt
     1621 attcagatcc caagtcacgt tggacagcac gtaaagggct gttttggggc gcggctgaag
     1681 gtggtccgat tgaaacgggg gaaattccgc caggcaatca gaacacggtc ggctttcgta
     1741 cctataaatc cgtttttgac ttaggtccgg cggcagagaa atacacagta aaaatgagtg
     1801 gctgccactc ttgcccgatc cgttgtatga cccaaatgaa tattcctcgg gtgaaagagt
     1861 ttggcgtgcc cagcacaggt ggtaacactt gtgtagctaa ctttgtccat accaccatct
     1921 tcccgaacgg gccgaaagat tttgaagata aagacgatgg tcgtgtgatt ggtaacctgg
     1981 ttggtctgaa tttgttcgat gactacggcc tatggtgtaa ctacgggcag ctgcatcgcg
     2041 actttactta ttgttacagc aaaggggtgt tcaagcgtgt tctgccagct gaagagtatg
     2101 cggaaattca ctgggatcaa ctggaagcgg gtgacgttaa tttcattaaa gatttttact
     2161 accgtctggc gcatcgtgtg ggtgagctga gtcacctggc tgatggttca tatgccatcg
     2221 cagaacgctg gaatttgggt gaagagtact ggggctacgc aaaaaataaa ctctggtcgc
     2281 cgtttggcta tccggttcac catgccaatg aagcgtcagc gcaggtcggt tccattgtta
     2341 actgtatgtt caaccgtgac tgcatgacgc atacccatat caattttatt ggttcgggtt
     2401 tgccattgaa actgcagcgt gaagtggcga aagagctttt tggttctgaa gatgcctacg
     2461 atgaaaccaa aaactacacg ccaatcaatg acgcaaaaat taaatatgca aagtggtcgc
     2521 tgttgcgggt ctgtttgcat aacgccgtca ccctgtgcaa ctgggtctgg ccaatgaccg
     2581 tttcgccgct gaaaagccgt aattaccggg gcgatctggc gcttgaagcc aaattcttca
     2641 aagcgatcac cggcgaagag atgactcagg aaaaattaga tttagctgca gagcgtatct
     2701 ttacgttgca tcgtgcctac acggtaaaac tgatgcaaac caaagatatg cgtaatgaac
     2761 acgatcttat ctgttcctgg gtattcgaca aggatccgca gatcccggtc tttactgaag
     2821 gtactgacaa aatggatcgt gacgatatgc atgcttcgct cacgatgttc tacaaagaaa
     2881 tgggctggga cccacagctt ggttgtccga cccgcgaaac attgcagcgt ctggggctgg
     2941 aagatattgc cgccgatctg gcagcacaca atctactgcc tgtgtaagga atgggtgaga
     3001 tgaaccatcg ggatgaattg ccgttagcga aagtcagcga agttgacgag gcgaagcggc
     3061 aatggttaca ggggatgcgt caccccgttg atacggtaac cgaaccagaa ccggctgaaa
     3121 tcctggcaga gttcattcgc cagcattctg cggctgggca gctggtggcg cgcgccgtgt
     3181 ttctctcgcc accctattcg gttgcagaag aggagctgtc agttttgctg gaaagcatta
     3241 agcagaacgg agactacgcg gatatcgctt gtatgacggg aagccaggat gattactatt
     3301 actcaacgca ggccatgagt gaaaactacg cggcgatgtc tttgcaagtt gtggagcagg
     3361 acatttgtcg cgccattgca cacgccgttc gttttgagtg ccagacatat cctcgtcctt
     3421 acaaagtcgc catgctgatg caagcgccat attacttcca ggaagcgcag attgaagccg
     3481 cgatcgccgc gatggatgtt gcacctgaat atgccgatat acggcaggtg gagtcatcga
     3541 cggcggtgtt gtacttgttt agcgagcgat ttatgaccta tggcaaggct tatggcttat
     3601 gcgaatggtt cgaggtagag cagtttcaga acccctgaaa tttcagggag atgtcatgga
     3661 tagggtggac agttgcagcc actgcattag gggataatca gatgtccttc actcgacgca
     3721 aatttgttct ggggatggga acagtaatct tttttactgg ttctgcgtct tctctgttag
     3781 cgaacacgag gcaagaaaag gaagttcggt atgccatgat tcatgacgag tcacggtgca
     3841 atggctgtaa tatttgcgcc cgcgcatgcc gtaaaactaa ccacgtcccc gctcagggaa
     3901 gccgtttatc gatagcacac attccagtca ccgataacga caacgaaacg cagtatcact
     3961 tttttcgcca gtcatgtcag cactgcgaag atgctccgtg catcgacgta tgcccgacag
     4021 gggcgtcgtg gcgtgatgag caggggatcg tgcgggtaga aaaatcgcag tgcatcggct
     4081 gcagttattg cattggcgct tgtccgtacc aggttcgcta ccttaaccct gtcactaaag
     4141 tggcagataa atgtgatttc tgcgctgagt cccgactggc aaaaggcttt ccgcccattt
     4201 gcgtcagcgc ctgcccggaa cacgcattga tctttggtcg cgaagatagc ccagaaattc
     4261 aggcgtggtt gcagcaaaat aaatactatc aatatcagct acctggtgcc gggaaaccgc
     4321 atctgtatcg ccggttcggt caacatttga ttaaaaagga aaatgtatga acccgtcgca
     4381 acatgctgaa cagtttcaga gccagttagc gaactatgtg ccacagttca ctcccgaatt
     4441 ttggccggtg tggttgatca ttgccggagt attgctggtt gggatgtggt tggtgctggg
     4501 gctgcatgcc ttgcttcgtg ctcgtggcgt gaagaaatca gtcaccgatt atggtgagaa
     4561 gatttatctt tactgcaaag cggtcagatt atggcactgg tcgaatgcgt tactctttgt
     4621 attgttgctg gcaagtgggc tgataaatca ctttgcgctg gtgggcgcaa ctgcggttaa
     4681 aagtctggtt gcggtgcatg aagtttgcgg atttttgtta ctcgcatgct ggctcggctt
     4741 tgtgctgatc aatgccgttg ggggtaatgg tcaccactat cgcattcgtc gtcaggggtg
     4801 gctggagcga gcggcaaaac aaacgcgatt ttatttgttt ggcattatgc agggggaaga
     4861 acatcctttc ccggcaacaa cccagtctaa atttaatccc ttacagcagg tcgcctatgt
     4921 tggtgttatg tatggattgc tgccgttgtt actattgacg gggctgctgt gtctctatcc
     4981 gcaagccgtg ggagatgtgt ttcctggcgt aagatactgg ttattgcagg cgcattttgc
     5041 tctggcattt ataagcctct tttttatctt cggtcatctt tatctttgca ccacggggcg
     5101 tacgccacac gaaaccttta aaagcatggt cgatggctat caccggcact aacgaatgat
     5161 cattacacga gcagacctgc gagaatggcg tatcggagcg gtgatgtatc gctggttcct
     5221 gcgccatttc ccacgcggcg gtagttatgc cgatattcat catgcactga ttgaggaagg
     5281 atatacggac tgggcggaga gtctggttga gtatgcgtgg aaaaaatggc tggcggatga
     5341 aaacttcgcg catcaggaag tttcctctat gcaaaaactg gccacagacc ccggagagat
     5401 accattttgc agtcagtttg cccgcagcga cgatcatgct cgcatcggct gttgtgagga
     5461 taacgcccga atcgcaaccg ctgggtatgc cgcgcaaatt gccagtatgg gttatagcgt
     5521 gcggattgga agtgtcggct ttaacagtca cattggcagc tccggtgaac gcgcacgggt
     5581 tgctgtaacc ggtaattctt cgcggataag cagcgctgga gatagtagtc ggattgcgaa
     5641 taccggaatg cgagtacgcg tttgtacatt gggtgaacgg tgtcatgtcg ccagtaatgg
     5701 tgacctggtg caaatcgcca gctttggcgc aaacgccaga attgccaaca gcggcgacaa
     5761 tgtacacatc attgccagcg gtgaaaattc aacggtagtg agcacgggcg tggtcgactc
     5821 aattattctc gggccagggg gaagcgccgc actggcatat catgacggcg aaagagttcg
     5881 cttcgccgtc gccattgaag gtgaaaacaa tattcgaacc ggagtccgct atcgtcttaa
     5941 tgaacagcat cagtttgttg agtgctgata gctttacctt taatcaaacg aaagacgccg
     6001 acgagcacgg gatgcaggct tcacgaccgc ccgagccatc gcctcaccaa tttctgcaca
     6061 tgccgtaagt ccctgaacga aaggctggtc gtgcatcaac cagggcaacg cgccgaacgc
     6121 tacgcgtcca cgaatatctt cgggttgctg taacgtataa tcttcgccaa tatcagggat
     6181 ttcatcccct gttttctgta attgctcgcg tagcccaggg aaaggaatat ctttcacttt
     6241 aagcggacgc tgtccgcggg catcaataaa aacgtcaaac gagtagctgt tgtcttccgt
     6301 tttcaggacg gtgcgcgact cattaatttc cattttgtag tcttcaccga gggcgagaat
     6361 atgaatgatt cccgcttcac gtaaggcaag taggcgacga atagactctg aagggattgc
     6421 cgcataatta tcgatgaaaa ccctggcaag gcctttactg aaccgtttat ggtcgtgttc
     6481 attcagatga ggaacaattt cctgtacggc ttcatgcagg cgcagaatga cataacgcca
     6541 gggaacagta tgtttttctc gtttattgcg ttcgacttcc tggagatttt tttctgccca
     6601 gtcaaatggg tcgcgttgtt tgcgctcggc aaaccaggct tgagcaaagg aatcgacatt
     6661 caggctctct aaggctatgc gttggctcca gtctggatca gcaaacttga tttcctctac
     6721 tatcaatcta aatacccgat ccaaaaggcc ctcttcacct ttttgaatct cagcatttaa
     6781 tgcctgatcg gtgacgatgt gtaagggctc gtagggaata gggcaataga aatcggcttc
     6841 gggtaaaata cccgtgcgcg acatcaacgt aatatttagc ttttcacttg cgttatcgcg
     6901 gttaaaaacg acgtgttgtt tatcatcttc aatgaacgaa ccatgctgaa tagccactgc
     6961 cattgccgca tccagtccgc tcaaggatgt tcccataata cccacgttac acgcatcgac
     7021 ctttgcttcc atcaggcctg accacggact gggaaaatac gttctggttg cttcttcttc
     7081 atcaggccag acgtgacccg tggcaatcac cgctaaatca aacgtctcgc tgggtaaatc
     7141 ctgatttgta gcgagcatga cgccagcatt tgtaatttgc agatcggtaa cctggcatga
     7201 ttcataaaca gccactgcaa atttttgctg tcgtgcctgg tctactaatc gtaaaaattg
     7261 atcgcggaaa tattcgccca gcagaattcg cggtaaaaac tgacgatcgt gcaaggtttc
     7321 ttttttaacg ccataacgct ggagatggct ggcttcttgc ttttgtagcc attcgagata
     7381 cgtacaatta atcggcggta tttcaatact ggcaatattt gccagcatca tttttgagtt
     7441 ttcctcatca ctgtatggca tcccgacacc ggcctcgtca gcctgctcga agatagaaat
     7501 agaaagtgga gtttgttgct gtagaagcga gaataaggtg tagatccccg taggcccggc
     7561 acccacaata gcaatttttt tcattgttac ttacctgtac gcattctttc gaatttagtg
     7621 ttatcagata agtttggttg agatttttag aatttgagaa atttcagaca ggtatgaaaa
     7681 gggcgctaac cgcccttgat taaattctgt caggcgagtt ttgcctgatt gatttgactc
     7741 agtggctcat tgacatcgaa aactttggcg acaacttcct caacgccgag atttttaagt
     7801 cgggcagtat gtttttccag ataggcaagg gcgctttttt catcagtaaa aagatagatc
     7861 ccaccggctt cgtggttctt ttcactttct gtccatacct tccacagaaa accaggttcc
     7921 tgattaatcg actcagcaag tggtttaagc tgctcagcca ttgcgtcgcc aaatgggcca
     7981 ttaaaagcaa aatgaagttg taacaaagtt gccatagcaa ttctccttct gataacgcat
     8041 acgaagacag tgtaaatctt ttaacgtatt ggatacagag aagaaacaga ggaaaaaagg
     8101 agaagaccag attaagaaaa tctggtgcgt ccgagtggac tcgaaccacc gacccccacc
     8161 atgtcaaggt ggtgctctaa ccaactgagc tacggacgca ggatggtgcg ttcaattgga
     8221 ctcgaaccaa cgacccccac catgtcaagg tggtgctcta accaactgag ctatgaacgc
     8281 aacgttgtag gtgacaacgg ggacgaatat tagcggcaga gtgggaaggt ggcaagaggc
     8341 aaaacgtaat tttctgcgct atttcgaccg tttgcagagc ttttaagcaa attggctata
     8401 ttttgttgat ttgcaagggc gatttttatt caggatcgca tttacatctg atacaaccca
     8461 ataattcgtg tatcagagtt taaacagagt tgtttgccac catctcaaat ctgtcgtgga
     8521 taaatcctcc gtagccttta cacttaggta gcaataaagg cacacagatg cagcaaagga
     8581 ataaatcatg ggatctgatg cgaaaaactt gatgagcgac gggaatgtgc aaattgttaa
     8641 gaccggcgag gtcattggcg cgacgcaact tactgaaggc gagttgattg ttgaagctgg
     8701 cggaagagcc gaaaataccg tggtcacggg ggctggctgg ttgaaagtgg caaccggtgg
     8761 gatcgccaaa tgcacacagt acggtaacaa tggcacgcta tcggtcagcg acggtgccat
     8821 tgccacagat attgttcagt ccgagggagg cgcaattagt ctctctacgc tcgctacggt
     8881 taatggccgc catcccgaag gtgaattcag cgttgatcag ggttatgcct gcggtttgtt
     8941 gctggaaaat ggcggtaacc tgcgtgtact ggaaggacat cgcgcggaaa aaatcattct
     9001 cgatcaagag ggcggcctgt tggtcaatgg gacaacctca gctgtcgtgg tagatgaagg
     9061 tggtgaattg ttggtgtatc caggtgggga agccagcaat tgtgaaatta atcagggcgg
     9121 cgtttttatg ctggccggga aagccagtga tacgttgctt gctggtggca ccatgaataa
     9181 tctcggtggt gaagactctg acactattgt tgagaatgga tccatttatc gtctggggac
     9241 ggatggcctt cagctctaca gttccggtaa gacgcaaaac ctgtccgtga atgtgggtgg
     9301 tcgggctgaa gtgcatgccg gtacgctgga aaatgcggta atacaaggtg gaacagtgat
     9361 cctgttgtca cccaccagcg cggacgaaaa ttttgtcgta gaggaagagc gcgcaccggt
     9421 tgaactgacc gggagtgttg cattactgga cggcgcttca atgattattg gctatggtgc
     9481 agatctgcaa caatcaacga ttactgtaca gcagggcggt gtgttgattc tcgacggcag
     9541 tacggtaaaa ggtgacagtg tcacttttag tgttggtaac atcaatctga atggcggaaa
     9601 actgtggctg atcactggtg cggcaacgca tgtgcaactg aaagtgaaac gcctgcgcgg
     9661 agagggagcg atttgcctgc aaaccagtgc gaaagaaatc tcacctgact tcatcaatgt
     9721 gaaaggggaa gttaccgggg atatacgcgt tgagataaca gatgccagtc ggcaaactct
     9781 gtgtaacgca ctgaaactac agccagacga agacgggatt ggcgcaacgc tccagcctgc
     9841 gtaaaaacac aggcgcaacc cgtgaaggtt gcgccttgtc tttattagcg ggacgctcgt
     9901 tgcagaatga tggctgacgg cagacgttgc aggaaccgca tacgcagcat catcataatg
     9961 gctgccgacg tcaggccaat aataaagcct atccagaagc ctgctggccc cataggttca
    10021 acgaccagat cggtcagtgc cagaatatag ccgcttggca agcccagcac ccagtaagcc
    10081 gtaaaggtaa tatagaaaat ggaacgcgta tctttataac cacgcaaaat cccactgcca
    10141 atcacctgga tcgagtcaga aatctgatat accgccgcca gcaacatcaa atgcgcagcc
    10201 agcgttacaa cctcgggatt gtcgttgtac aacagggcga tttgctcccg cagtgaaacc
    10261 gtgaatatgg ccgtcagggt tgccatacag acacccacca taagcccggt ccgcgcagcg
    10321 gtttgcgcat ccagcgttga gccctgaccc agacgataac ctacgcggat agttaccgct
    10381 gccgccagcg acatgggaag cacgaacatt agtgaactaa agttcagggc aatctggtgt
    10441 cctgcgacat caacaatacc gagcggagac actaacagag ccacgacagc aaacagtgtc
    10501 acttcaaaga acagtgccag cgcaatcggc aaaccgagtt gaatcagtcg tttcataacc
    10561 gcaggatcgg gtttcgcggt gcctttttcg ttacgaatat cgcgcatgga gcgggcgcgt
    10621 ttaatgtaag aaaccatggc aaggaacata acccagtaca ccgccgcagt agccacgcca
    10681 caaccaacgc caccgagctc aggcataccg aaatgaccat aaataaagat atagttcacc
    10741 ggaatgttca ccagcaggcc gataaagccc attaccatac ccggcttggt ttttgccaga
    10801 ccttcacact ggttacgggc aacctggaag aacagatagc ccggcgcgcc ccacaacaac
    10861 gcacgcagat aacccacggc tttgtccgcc agagccggat cgatgttttc catggagcgg
    10921 ataatgtaac ctgcattcca cagcaccagc ataatgagaa cggaaacaaa acctgccagc
    10981 cagaaacctt gtcgcacctg atgcgcaatg cgctcacgtc gaccggaacc atttaattgc
    11041 gcgataaccg gcgttaatgc cagcagcagt ccgtgaccaa agaggatcgc cggaagccag
    11101 atagaagtac cgatagcgac cgccgccatg tcggtggcac tatagccgcc cgccatcacg
    11161 gtatcgacaa aacccatcgc agtttgggcg atttgcgcga gaatcaccgg gattgctaat
    11221 gctaataaca gacgcgcttc actgatatac ttctgcacgt gaacaccttt tatttgtagt
    11281 tatatgagag actaaaaagc cgcctgaatg ggcagcaaaa agaagaagca ggggaaattc
    11341 agtctattgt agcgaggatt tattaattct ccagtgaaaa aatagtcatt cttacccctt
    11401 cactggtaag ggcgtttgcc acctgctatt gtgctgaaca gaatgtctta actgatttca
    11461 ggagttgtaa gtatgtttac ggggattgta cagggcaccg caaaactggt gtcgattgac
    11521 gagaaaccaa attttcgtac gcatgtggtg gagttacccg accacatgct ggacggcctg
    11581 gaaaccggtg cgtccgtggc gcataacggt tgctgcctga ccgtgacgga aattaacggc
    11641 aaccatgtca gttttgacct gatgaaagaa acgttacgca ttaccaatct tggcgattta
    11701 aaagtggggg attgggtaaa cgttgagcgt gcggcgaaat tcagtgatga aattggcgga
    11761 cacttaatgt caggtcatat tatgaccact gctgaagtgg cgaaaatatt aacctcagaa
    11821 aataatcgcc agatctggtt taaagtccag gatagtcagt tgatgaaata tattctgtac
    11881 aaaggattta ttggcatcga cggtattagc ctgaccgtcg gcgaagtcac gccaacgcgt
    11941 ttttgcgtcc atttaattcc ggaaacactg gaacgcacga ctcttgggaa gaaaaaactc
    12001 ggcgcacgcg tcaacattga aattgatcca caaactcagg cagtggtaga tacggtagaa
    12061 cgtgtgctgg cggcacgaga aaatgccatg aatcaaccag gtacagaagc ctgatctgaa
    12121 ctcgcccccg aagagggggc gatagaatag cctttagcga gcgactcgaa ggccgttttc
    12181 aacaccgcgt gagaacacca cctgccagag ctgaatatca cgggcgcgga aagcacctgc
    12241 acaagcattc agataatagg taaacattcg tttaaagcgt tcactgtagt tatctgcaat
    12301 ttctggccat gcggcgagga atcgttcata ccacgccatc aacgtagtat cgtaatcagc
    12361 accgaagtta tgccagtctt ccatcacaaa gtggggttcg ctggactgag caatctggcg
    12421 tacagagggc aggcaaccgt tcggaaaaat atatttatta atccagggat caacattcag
    12481 atcggttttt ttcgaaccga tagtatggag caggaatata ccttccggtt tcaaattacg
    12541 atccaccatc gcaaaatagg tatcgtaatt tttcggtccg acgtgctcga acatccccac
    12601 agaaacaata cgatcaaact ggtcgttcag gtcacgataa tcttgcagca aaatggtgac
    12661 atccaggcct tcacagcgtt cctgagccat tttttgctgt tcggcagaaa tggtgacgcc
    12721 gaccacgctt acgtcatagt tagatgccat gtagtgcgcc agtccgcccc agccgcagcc
    12781 aatatccagt acgcgcatcc ctggttttaa ctgcaatttt tcacaaatca ttttgagctt
    12841 cgcctgctgg gcagattcca gattatcggc atctttccag taagcgcagg aatattgcat
    12901 gaagggatca agcatgcgac tgaacaagtc attacccaaa tcgtaatgct ctttgccgac
    12961 tatccaggca cgttttttac tctgcagatt gaagagacgg gcgcctgcaa tacgcagcgt
    13021 gtctttgaaa tgatggggga gttggttctc gagacctgcg cgtaagactt tgctaaaaaa
    13081 catatccagt cggtcacatt cccaccagcc atccatataa ctttcgccta accccaaaga
    13141 gccttcttgc agaacgcgtt taaaaaaatc ggggtttttc acacgaatat ccgccggggc
    13201 agaaccgtta atggctatac cggcacggct aagtaattcg ttggcgatac ggtaccagtt
    13261 gtcatccggt acactgactt cttctataca cgatgaactc atagtttctc catcactggt
    13321 cggtgatcag aaccttaaaa cagcgtagac gctttttggg ctttgtgaga aatctcgcag
    13381 aaaaaaccgc gagtctataa tccgacgtag aacagaggga aaggaggaac ccttgccgaa
    13441 atgaccatcc atagtgagtc gggaacgatc ctgtccccgt aaaaacataa tatttatcgt
    13501 agctttaaca actcgaaatt agtataggcc gctaatttca atcatacaac aaaattttgt
    13561 tagcagccta agtataagta tatcgatata gatcagtgtg attcgctatg agcgacttcg
    13621 gcattgccat gattctggca gccaacttct tcacaacgtt gcatcatgta acccagcgcg
    13681 accagcacta ctgttgataa catcacgctg gtggtggtga gcaatggcgt gctgatactg
    13741 atcagccagg aaactaccag acttgcgagg aagcacagac ccagttgcag agtgttctgc
    13801 aacgctgcgg cgcgaccggt tgcatgtggg aagggacgca gcgcctgggc gacaacaata
    13861 gggtagatcg cgccattggc aatcgccatc acacagaatg ggatcaggat ttcgaccagc
    13921 gacacatggc taatgaagcc cgcagcccag gtcgcaatga cgctgacagc aaacagcacc
    13981 agcaaccacg gtaataactg cttgccttgc catttctgca gtgcggcgcg acagccataa
    14041 ccaccaatca gaaacgcgat agtttgcggg acataactta aaccaataac tgccgggctg
    14101 tagcccattt cactaaggat gaacggtgaa ccggtcagcc atgcaaaaaa actggctgaa
    14161 caggctgcat atatcagcac gttgccgcga taggttttag aacgtagcag gtcggtaaag
    14221 gtcagaccat cctgactatt gttacgggcc ttcgtcgtgg gtttgagcca gaaaataggc
    14281 agaatcagca ccacggtaat ggcaaacagg gtggcgaaaa tcgcctgcca ggaaaaatgg
    14341 accagcagcc agcttcctaa caggggagcc agtgccggag acagacccac cagcggcatg
    14401 atggtcgcaa aaatacggtt gactttctgt gaaggataat aatctgtcac taatgcttgc
    14461 cagataaccg ccgcggcgca gacccccaca gcctgtacaa aacgcaatac cagcagcgta
    14521 gcggcgtttt ctacccacag catccccaga ctacctaacg caaaaattgt caggccgatt
    14581 aataacaccg gtttacgacc ataacggtcg gagagtggcc cccacagaag ctgggctgcg
    14641 gcaaaaccgg caaggaacag actaaggctg gcactgacag cagacgcagg cgtttgcagg
    14701 tcggcctgta tggcggcgaa agcaggcaga tacatatcgg ttgccagaaa accgagtacg
    14761 ctcaaacccg ccagccagac taaaaatctt ttcccaggtt gcatgtatat tctctaaatc
    14821 tctcaattaa acaccgccgc aaagtgtagg gagtgaaatt agtgttgtga aacggtaata
    14881 tttgatgact ggtttcaaaa attttgcagg cagactatgt ggtcagaata ttcactcgaa
    14941 gttgttgatg cggtagcgcg taatggtagt tttagcgctg cggcacagga gctgcatcgc
    15001 gttccttctg cggtcagcta taccgtgcgt cagctggaag agtggctggc ggtaccgctc
    15061 tttgaacgac ggcaccgtga tgtggagctg accgctgctg gagcgtggtt tctcaaagaa
    15121 gggcgctctg ttgtcaaaaa aatgcagatc acccgccagc aatgtcagca gatagcgaac
    15181 ggctggcgcg gtcagttagc tatcgcagtg gataatattg tcaggccaga acgtacacgg
    15241 cagatgatcg ttgattttta tcgccatttt gacgacgtcg aacttctggt ctttcaggaa
    15301 gtgtttaacg gtgtctggga tgcgctttcc gatgggcgcg tggaactggc gattggcgca
    15361 acacgcgcga ttccggtagg cggtcgttat gccttccggg atatggggat gctaagctgg
    15421 agttgcgttg ttgccagcca ccacccgctg gcgttgatgg atggcccgtt cagcgatgat
    15481 acgttgcgca actggccgtc gttggtgcgc gaagacacct cgcgaacgct acctaaacgt
    15541 attacctggt tgctggataa ccaaaaaaga gttgtcgtgc ctgactggga atcatcggca
    15601 acctgtatct cggcaggatt atgcataggg atggtgccaa cacatttcgc caaaccgtgg
    15661 cttaatgagg ggaagtgggt ggcattagaa ctggaaaacc cctttccgga ttcggcatgt
    15721 tgtctgacat ggcagcaaaa tgatatgtcg cctgcgttaa cctggctgct tgagtatctc
    15781 ggcgatagtg agacgctgaa taaggagtgg ctgcgggagc cggaagagac tcccgcaacg
    15841 ggtgattaac gacgatagtc gcggaacggg ccgtcagcca cggagcggcg ttcaatcaag
    15901 cgcggatgca cttcaatgga ctgcggttct tcacgtttgt tgacgatacg atccaacagc
    15961 atgttgaacg ctgtttcacc cagcgaatct tttggctggt ggatcgtggt cagcgccggc
    16021 gtaaaatagc gggcgttgcg cacgttatca taaccgatca gcgaaacatc ctgcgggacg
    16081 cgcagaccca tttcatcagc agcacaaagt gcgcccattg ccatgatatc gccaccacag
    16141 aagacggcag tagggcgatg cggctgcgac agtatttgct gcatggcgcg ataaccagat
    16201 tcgggttcaa agtcaccctg aacaatccag ctttccggca ccttgatcat cgcttcttcc
    16261 atcgccttca taaaaccggc aaggcggcct gcgccggtgt tacgttccag cgggccgggg
    16321 atgacgccga tttcgcggtg accgcgttca atcagataac gcccggccat gtagccgcct
    16381 tcgaacgcgt tatcaatgac cgcatcggtg aagtcagctt ttgcttcacc ccagtccatc
    16441 accaccattg ggatatggcg atactcttcc agcatcgcca gcaacggctc tgggtactca
    16501 gaacacatca ccagcagacc atcgacgcgt ttttgcgcca tcatcgacag ataagcccgc
    16561 tgtttctcaa gattgttcca cgcattaccc agaatcaggg tgtaaccttt ctggaagcaa
    16621 tttttttcaa ccgcttcaat gatctcggca aaataggccg cttcgctgct ggtcgccagc
    16681 aaaccgatag acttggtgtg gttaaccttc aggctacgcg ccaccgcgct aggggagtag
    16741 tgtaattctt taatcgctgc ccacacggcg ttgcgcgttt cttcagcgac gaaacgtgtt
    16801 ttgttgatta cgtgtgacac agttgtagtg gaaacgtttg ctcgtttcgc tacatctttt
    16861 attgttgcca ttccatttca ctccagaccc taacttcagc tcctgcaaaa tcgcaaggta
    16921 aacgtttgcc tttacacacc ttacgcacaa caaaagtgtt gcggtacgcc ggaaaaacga
    16981 cacggagcgt caggaagggg tcaatggcca gtacgcaaat agtgtaagcg tggaattttg
    17041 gccgatcttg acgaaaaggg gaagtggtaa aaacacttat cattataaat gacgggagat
    17101 tttttcatca cagtgtgtaa aaatgcgtaa tatcactatt tgtgtggtaa taaaaaagga
    17161 gaaaacttga tgagcaccga ccttaaattt tcactggtaa caacgattat cgtcctcggt
    17221 ttgatcgtgg ccgtgggttt gactgccgcg ctgcactgat ttcctctcga gggagcgcgt
    17281 tctccctcgc atctccttcc tgtttgttag caaaatggca aaagtgtttt ctctttttgt
    17341 tattttcatt tttttgtgat tgctgtcatg ctttcgccac tttaatgaat atcgcgtaat
    17401 gacgcgaaga ggtggagata aggcatgaaa attaactatc cgttgctggc gctggcgatt
    17461 ggcgcgtttg gtatcgggac aacggagttc tctccaatgg gtttgttgcc cgtcattgcg
    17521 cgcggtgtgg atgtctcgat tcccgctgcc ggaatgttaa tcagtgccta tgcagttggc
    17581 gtaatggttg gcgcgccgct gatgacgctt ctactttctc atcgtgcccg ccgcagtgcg
    17641 ttgattttcc tgatggcaat tttcacgctc ggcaacgtac tttccgccat cgcgccggat
    17701 tatatgaccc tgatgctttc acgcattttg accagcctga atcacggagc attttttggt
    17761 ttgggttcag tcgtggccgc aagcgtggtg ccaaaacata aacaggccag cgcagttgcc
    17821 actatgttta tggggttaac cctggcaaat atcggtggcg tgccggcggc gacctggttg
    17881 ggtgaaacca tcggctggcg gatgtcattt ctggcaacgg cggggctggg agtgatttca
    17941 atggtaagtc tgttcttctc attacctaaa ggtggtgcag gggcacgacc tgaagtgaaa
    18001 aaagagctgg cggtattaat gcgtccgcag gtgctgtctg cattgctgac gacggtactg
    18061 ggagctggtg caatgtttac tctctacacc tatatctctc cggtactaca aagtattacc
    18121 cacgcaacac cagtgttcgt cacggcaatg ctggtgctga ttggtgtcgg attctctatc
    18181 ggtaactatc tcggcggcaa actggcagat cgttcagtta acggcacgtt gaaaggcttt
    18241 ttgttgttgt tgatggtgat tatgctggca atcccgttcc tggcccgcaa tgagttcggc
    18301 gcagctatta gcatggtggt gtggggcgca gcaacctttg cggtcgtacc gccgttacag
    18361 atgcgcgtga tgcgtgtcgc cagtgaagcg ccaggtctgt cttcatcagt caatattggt
    18421 gcctttaatc ttggaaatgc gctgggagca gctgctggtg gtgcggtaat ttccgctggg
    18481 ctgggataca gctttgtgcc ggtgatggga gcgattgtcg cgggactggc attattgctg
    18541 gtgtttatgt cagccagaaa acaacctgaa acagtttgcg ttgctaacag ctaattaaac
    18601 aaaaacgcag aaggataacc ttctgcgtta cagttgctga caacgtgcga tttatcttgc
    18661 cgaatgcgct gttaacgcct catccgcgtt ggcatggtaa gcgaagcgca tcaggcaatg
    18721 ctgcatttgc catcagttat tatgcagcga gatttttcgc tacgaattcc cagttcacca
    18781 gcgcccagaa gtgctccaga tagccaggac gtgcattgcg atagtcgatg taataagcgt
    18841 gttcccagac atcaacggtc agcagcggag tcgcatcggt ggtcagcgga gtacccgcgt
    18901 tagaggttga aacgatagcc agtttgccat cgctgttttt caccagccag gtccagccag
    18961 aaccaaagtt tttgatcgct gcatcagtaa actgcgcttt gaaatcggca aagctaccaa
    19021 aagatgcggc gatagcttca gcgacttttc cagtcggttc gccaccggcg ttcggtgcca
    19081 ggcagttcca gtagaaagta tggttccaga cctgagctgc gttgttgaat acgccacctt
    19141 cagagctgcg aataatctct tccagtgatt taccttcaaa cgcggtacct ttaatcaggt
    19201 tgttcaggtt agtgacataa gtctgatgat gcttgccgta gtgatactcg atagtttccg
    19261 cagaaatgtg cggtgccaga gcatctttag catatggtag tgcaggtaat tcgaatgaca
    19321 ttgctactct cctttattat taatttgcat acgtacaata gccttattgt gcgtatggat
    19381 agggtagcaa attaaaaggt gagacaaaag atgaacttac cctgttgccg taacaacagg
    19441 gtaaagtttt agcgaagtgt ttttggggtc attacccgac gagcgccaac atagtggcgc
    19501 tgccagtagt cttcactgag agaagtgatt tgaatttcct gacctgtgcg cggtgactga
    19561 ataaatttgc cgttgccgac atacacgccg acatgatcgg ctgtgccgcg tccctgagta
    19621 cggaaaaaga ccaggtcgcc gtttttcagt tcactacgtt cgattggcgc tgcatcacgc
    19681 aggtgataca tttcattcgc cgtacgcgga atacgaattt tcaccaaatc tttataagcg
    19741 taataaacca ggccgctgca atcaaaaccg gtacgcggtg agctgccacc ccaacgatat
    19801 ggcttaccaa tttgctgcat cagtttattc attgccactt tcgtagcttt ttgcaccttc
    19861 gctttgtgcg cgtcggcaag agttcctgac gcggcatttt tgactttcac acaatgcgat
    19921 ttatagccct tacgtttggt acacttttcg gtaaagctgg ctgaggccgt tttattgacg
    19981 gcgtgtttgc tgcgagtttt ggcgttttta gaagcggtaa tggaagattt tttaacggaa
    20041 gaagcggttt tgcttttggt cgtactttta ctggcggctt ttttcgctgt tttctggctg
    20101 gctttttggg tctttttggt ggttgccgtg cttttctttt tatctgcttt tttggtgata
    20161 tgagtggtag cagaactctc cctggcttgc tttgaagcat gggccatagg cgttaaaggc
    20221 agggtggtaa aaagtaaagc acagagcgtg atcgaaatac ggtttatccg cgccactgag
    20281 cgtccctctg ttaaggcagg tctatcatgc ctgcgtctga tttacaaaac ttgatgattc
    20341 taatgcatag aagaagagaa agttaatagc ttttattgtt aaaaaatacg tttcgttggc
    20401 gagcgcaaaa aaatgcagca gtgaaagcta aaaatggtta taaatacgcc agtttgccga
    20461 gcatctggga agaaacgaat atttaaaatg caacaaatag agcaaaggga taaaatagca
    20521 aaagcgtgat gaaaatgtta ttttccgatg ccgcactgac ccgctacaat gtcagattac
    20581 tgccgtaaag aagtttgagg aagcaagaca atgagcacca ctatcgaaaa aatccaacgc
    20641 cagattgctg aaaacccgat cctgctgtac atgaaaggtt caccgaaact gccgagctgc
    20701 ggtttctctg cccaggcagt ccaggcgctt gccgcatgtg gcgaacgttt tgcctatgtt
    20761 gatattctgc agaacccgga cattcgtgcg gaactgccta aatatgctaa ctggccgacc
    20821 ttcccgcaac tgtgggttga cggcgagctg gtaggcggtt gtgatatcgt gatcgaaatg
    20881 tatcagcgtg gcgaactgca gcagctgatc aaagaaactg ccgctaaata caagtctgaa
    20941 gagccggacg cggaataatc cgtcctgcaa tactgacgga tgcgaacggg catccgtcag
    21001 tcctttctct atccccaatc cagcccttgt ggcgaacttg aaaatcccgc ctcacgcagc
    21061 agcgtaaaca tcggcgtttg ccggaccggt agatcattca cttctgttag cgtaaagcgc
    21121 agccgtggtt cgcgacgcag tgcggtagtc agcgcgtgga aaacctctgg ggcgagtaat
    21181 tcctcttttt cctgccagac cagcattttt ttgccacctt gcgccagata gagtaacaat
    21241 ttgccgccag aaacgaccac cagcgcaccc gcccgacgcg ttggaaccag cgttgccgga
    21301 tgtgcaggcc agggcagaag atttccccac acattagccg gatcgttggc agagagcgcc
    21361 actggtgtat agtggcgcgt ttgcgtggct tgtgtcgcca gatcgcgcaa tcggtcaata
    21421 gtcagacgtt cagcgaattg cgcgccaccc agaccttcta caaaacgacc tcgcataatt
    21481 cgcccggagt cttccatact tcgacaaagc gtttgcatcg atggaaaccc gccagggata
    21541 ttttcggcta tcaccgcctg acgactgatg atgccgtagc ggtcgagcat attttccgcc
    21601 agcgccagca tcctttcggt atcgtttagt ggctccacct gcaataacga ccagcgtcca
    21661 gccagatttg gtgtgttgta agataccagc ggcgagacgg gttgcgcata gacaggacgt
    21721 ccacgacgag cccggtgact gcggcgagtt gaggtgcgtg cgttggaact gctgcgggtg
    21781 agggcgcgta acggtgccca aatgtcgctg gtgatgacgc cttgccagac cagcgcccat
    21841 aacgcctctt gcagggcaga gagatcaacc gattcgccga ttttgtcgcg tatccgttgg
    21901 ctgatttgtt gtgcaaacca ggctcctccg tcagccagaa cagcgattat cgcttgctgc
    21961 agcgccgaac gattcgcctg atccgcttcg gcgggagtga acgattctgc ggcatattcc
    22021 tgtagatgca gtgccaccag gccgtcatct tcacccagct ttttttgtcc cgaccagata
    22081 accgctccgg ttgccagtaa ttcatcgagc atttctggcg aatagtcgcg tacgcgggca
    22141 ggcaggatct ggctttccca gagtgaggcg ggtaaaccga ctccggcaag ctgttcgatc
    22201 acccgcatca cgccatctac gccctcataa acgcctggcg atgttgaggc aaagagcgcc
    22261 gggctaccat cggtggcggg taatacgccc tgacgttcca gcagcaatcg cgcataggtc
    22321 gtggctgcaa cgggacgcgt cgcttctctg gcggcttgca gcgagcgcaa acgcagacga
    22381 cgaaatactt catcgctcac ccagatgtct tgttgcagat tcatcaccag accctgttca
    22441 cgcagttgct gaagctgctc ttcgacaatg gcaataccga gactaaactc atgagccagt
    22501 tgttcagccg tgaccagagc atgagcccgg agatagcgca gaaagaggtc gcgaagcggg
    22561 taactgactc tatgtaaata aatctctggc aatgactctg gtagtcgtac gccgagggca
    22621 tcacgcagcc tggcggcatc atccatacag gcaagacgct cctgtccgct aatcatcgct
    22681 gggaagattc gttttactgc aagaagattt tccagatacg acgcaacctc ttcactgctg
    22741 cctgtatgcc gttgcgccag gtcttcaacg gtcattggcc ccagttcgcg cagcaggtcg
    22801 aacaatcctt cttcaccttt cgctcttctg ccaggagcca gtcgttgcaa ctcttcttcc
    22861 acctggcgaa tgacctgcgg gtcgagtaat tcccccggat cgacctgtcc gagtagattg
    22921 cgcagtaact cgctgtccag cgacagtacg gatgcccggc gctctgccag cggggcgtcg
    22981 ctctggtaca taaattccgc gacatagccg aacaataaac ttgtggcgaa aggcgagggc
    23041 gtagtggtcg ttacatcgga tatttgaatt tcgccaccgt tcaggcgacg catcaaacgt
    23101 tccagtgcgg gaagatcata aacatcttgc agacattcgc gtagggtttc gagaatgacc
    23161 ggaaaatccg gatatccctg agcgatttcc agcaactgac tggcgcgcag ccgttgttgc
    23221 caaagcgggg tgcgatggcc cggagtgcgc cccggcatta ataatgcccg cgcggtgcat
    23281 tcgcgaaaac gggcggcgaa aagtgccgag ctgcctaccg cctcgcggac aatttgcagc
    23341 aacttttctg gttcaaacaa aaaaatcgcg gcgtcgggca atttaccatc agtgtcagga
    23401 atgcgcgcaa caatgccgtc atcactggcg accacagacg cgtcagcgcc ccatagggca
    23461 tgtattcgcc cggcaatcgc cagcgcccag ggttcatgca cccgtcttcc atagggagag
    23521 tgcaaaataa tacgccagtc gccaatttca tcacggcacc gttcgaggac cagatggcga
    23581 ctccccggaa cgatgcccgt cgcgttgcgt tgctcgtcaa tcaacccctg aatattggcg
    23641 ttcgtatttt cttctgccag ccacggggga atagtcccgg aaaagaacgc gccatccgcc
    23701 agtaaatgaa gaaaatcgcc gatcatctcg cctaattcag ccggacgtcc gttaccttca
    23761 ccacgccaga aggggagccg ggcagaacga cccggagcag gagtcacaat cacctgatcg
    23821 cgggtgattt gctggatccg ccatgaggta gcgccgagag tgataatgtc gctcacccgc
    23881 gactcatata ccatctcctc atccagttca cccacccgcc gcgaaccggc ctgttcttca
    23941 ccttcgggta ataacacgct atacatgcca cgatccggaa tggtgccgcc gctggtaacc
    24001 gccaacaatt gagcgccagg tcgggcggtc aatatcccgg tctccctgtt ccagaccagt
    24061 ttggggcgaa aagcagaaaa atcgccagag ggatagcgcc cggaaagcat atccagcgtg
    24121 gcgtcaaaaa cacgtcttgg cagatctttc cacggtgcgg cacggcgtac gcgggagtac
    24181 cattcgtcta cctgtaatgc atccatcgcc gcggcggcaa cggtttgctg cgcaaggacg
    24241 tcgagtggat tatgcggcgg tgtcaggttt tccagcctgc ctgcgaacat acactctaca
    24301 atgactgcgg aatcgactaa gtcacgccgg gtacgaggga aaaacagccc tttagataca
    24361 ccgccaacct gatgtccggc gcgaccaatg cgttgtaacc cactggcaac agaaagcggc
    24421 gttgccacct gaatcaccag atccaccgcg cccatatcaa tccccagttc gagactggag
    24481 gttgcgacca cgcagcgtaa ttcacccgat ttcagcgcct gttcggtgat tgctcgttgt
    24541 tctttagaga cggagccgtg gtgtgagcgg gcaataaaaa cgtcgctact ttgtacatgg
    24601 ttagaggttg cgccggaggt cgactcgaaa tgggccgtat caacggcgat agacggggaa
    24661 cgctgtaagc gtgcggcgta aagctcattt aatcgtgccg tcagtttttc cgccagccca
    24721 cgtgaattag taaagacaat ggtcgagcga tggcgcaaca cttcatcaag gatacccgtt
    24781 tcaatatatg gccagatgga gccttcccgg ccggcatggc tgtcttcgcc ggtgccgctg
    24841 gcgaccgatg agacatcatc catattggcg accggtacga caattcgtat ctgcggatgg
    24901 cgcattgcgg gcgggttgac taccgtaacc gggcgatcgc caccaagaaa tgctgccaca
    24961 tcgctggctg agcgcacagt ggcagaaagg ccaattcgct gtgctgaggt gtggagcagc
    25021 gcatcgagcc gctccagact taacgccaga tgcgcaccac gtttactgcc cgctaccgcg
    25081 tggacttcat caataattac cgtttcgacg ccgcgtagcg tttcgcgcgc gcgggaggtc
    25141 agcatcagat agagtgattc gggtgtggtg atcagaatat ccggcggatt acgggtgagt
    25201 ttgctgcatt cctgtgcagg cgtatcgcca gtacggatcc ctacgcgaag attgacttcc
    25261 gtttcgccgc gccgccgccg ttcatcggca atacccttca acgggagctg caagttgcgc
    25321 tgaacgtcgg tgcccagagc ttttatcggt gaaatataga ggatgcgtga ggttttacgc
    25381 ttatgcgcct cgcgggtatc ttcgccgcct tcgcggaaga gccgatcgag ggcgtagaga
    25441 aatgctgcca gcgttttccc ggagccggtc ggtgcaatca ccagcgcatg ttcgcttcgc
    25501 gccgccacat gccaggtttg cgactggaca gcggtcggct gtttaaaggc gcgaagaaac
    25561 cagtcgcggg tcgccggtga aaacacgtcc ggcaggagcg atgaagggtc tggattatct
    25621 gccattaacg ctattccccg tgaatatcat gcttgatgcg tggaatagcc tcactataga
    25681 tgcctgaatc attgcatgtc atcaggcatc gactcgatta cacctcttcg gcggcaggta
    25741 gcggccagcc tcccagacgt ttccagcggt tgacgatttc acaaaacagc acagcagtgc
    25801 gttcggtgtc gtacagcgcg gagtgcgcct gggtgctgtc gaagtccatg ccagcggtct
    25861 ggcaagcctt tgacaatacg gtttgtccga gtgccagccc ggccagtgca gcagtgtcaa
    25921 aagtggcgaa agggtggaac gggttacgtt tcagtgaggc gcgttctgcg gcggccatca
    25981 taaagctgtg atcaaaattg gcattgtgcg ccaccataat ggcgcggtta cagccgctcg
    26041 ctttaatacc tttacgtaca actttaaaaa tttcgtgcag cgcctcgtat tcgctgaccg
    26101 cgccgcgatc gggatcgttc gggtcaatgc cgttgaaggc gagggcctct ggctgcaaat
    26161 ttgcgccgac aaatggttcg acgtggaaat gtaatgtggt gtccggcatc agccagcctt
    26221 gttcatccat tttcagggtg atggcggcaa tctcaagcag cgcatcggtt ttggcgttaa
    26281 atccggctgt ttcaacatcg atcacaacag gataaaaacc acgaaaacgg tcgcacagac
    26341 cggtaagttg agcgttatcg gacatctgat tctcttaata cggataaatt gcagcgcgca
    26401 ttatgacaaa tattacttgt cgatgcagta aagatgcggg cgcgatgagt tcacgcccgg
    26461 caggagatta gttgcccaga ccgcgaccgg cgtctttctc ttcgattaac tcaattttgt
    26521 aaccgtccgg atcttccaca aacgcgataa ccgtagtacc gccttttacc ggacccgctt
    26581 cacgggtcac gttacccccg ttttgacgga ttttttcgca cgcttcagcg gcgttatcta
    26641 cgctaagcgc gatgtgacca taagcagtgc cgagttcgta tttatccacg ccccagttgt
    26701 aggtcagttc aatcaccgct tcttcggttt ccgggccgta gccaacaaac gccagtgagt
    26761 atttgtattc cgggttttcg ctggtacgca gcagtttcat gcccagcact ttggtataaa
    26821 aatcgatgga gcgttgcaga tcgccaacgc gcagcatggt atgaagaaga cgcatctttt
    26881 tatcctcaaa atggctaaca gattcaaaat gttgttttag tatagcggcg gctttacgcc
    26941 gctcgcaatg ttggattaca acgtcgggta atcggtatag ccttccgcgc cgccaccgta
    27001 gaaactttcg gcacgctgtg ggttaagctc agctttgcgc tgcaagcggg cgaccagatc
    27061 cgggttcgca atccagtcac gaccaaatgc caccgcatca attaaccctt tgccgatcag
    27121 cgtttcagct ttttctactg tgtatgcacc tgcgccgata atcggaccgt ggaaacgggc
    27181 gcgtactttt tcgcggaacg catcagtata cggttcaccc cccgcccaat ctggttctga
    27241 catatgcaga taagcaatgc cgcgtttacc cagttgttca atcagataca gtgcatcggc
    27301 ttcttcattc gcgccgttat ccgtgttctg gaaagtaccg attggcgaaa cgcgaatgcc
    27361 aatgcgatcg gcaccccatt cttcaatccc ggcatcgacc acttccagta ccagacgtgc
    27421 gcgattttcc acgctgccgc cgtactgatc ggtacgatgg tttgaagaag gagaaaggaa
    27481 ctgatgcagc aaataaccgt gagcagagtg gagctctacc agatcaaaac cggcttcacg
    27541 cgcgttagca atggcctgac ggaaatcatt gacgatacct ggaatctctt ccagttcaag
    27601 cgcacgcggc atggatgttt caacacggat cgcctgacca ttttcatcgc gcagagaagt
    27661 acgtgttccc gcgctaagtg ctgaaggcgc taccggtgcc tgaccgccag gttgcaggct
    27721 ggcgtgagaa atgcgtccgg tgtgccacag ctgcacggcc atatgaccat tttcagcatg
    27781 aacgccagcg gtgatttttt tccatgcggc aatttgctcc ggactatgga tgccaggcgc
    27841 acctgcatat ccttttgcct gggcagaaat ttgcgtggct tcactaataa tcaaaccggc
    27901 actggcacgt tggcgatagt attccgccat caacggggta ggaatgtcac ccggttcaat
    27961 actgcgcaga cgcgtcagcg gtgccataaa aatacggttt gccgccgtga tcgcgcccac
    28021 tttcagtggg gaatacagtt tttcagatga cataaaggct cccggtcgtc tggtgataaa
    28081 aaatgcctgc taaacggcag gcgtcgcaat aatgtttttt acatgggcca gcgcgttttc
    28141 cagtggctcg aaactgcgcg aaattttggc ctgcagattc gcacccagcc atagtgcgta
    28201 aagcacttgt gcctgttgta gcggttcgcc acaaaaggtt aaacaatggt tctcacggcc
    28261 attttccagc gcctgagaga gcagggcgat cacgccgcga gcacctttat ccatcgcgct
    28321 gcgcatatct tctgacagat cgcacacttc ggcagagagt tttactgtca ggcaaccact
    28381 gatggttcca tgttggcaaa actggttcag tgtttgctgg taataagcca gtatgcggtc
    28441 gcggtagtta ccttcgccgg attgcagcaa ctcagtcagt cgctggtgat atgcggcgta
    28501 atgacgctca agcatggcaa cgccaaacgc ttctttagag cgaaagtagt gatagaagga
    28561 ccctttcggc acttcagcgg tttttagtaa ttcgcttagc cccatcccgg tgaatccacg
    28621 ttgcaggcaa agttgctcgc ccgtcgccag gagatgttcg cgagtatcat gttcggtgtg
    28681 tttgttcatg cgttggagtg tagtagacca gtcggtctag tgcaagcaaa gttgctactt
    28741 tggacattaa cagcttcaat aatggtaata atcttatctg aggagggcga agtggcggag
    28801 caattagagt tctttcccgt ccagagcccg tgccggggaa tttgccagtc tgacgaacgc
    28861 gggttttgtc gcggctgttt tcgtagtcgt gatgagcgtt ttaactggaa taaaatgagc
    28921 gatggcgaaa aacaagaggt gctgcgcctt tgtcggcagc gattaatgcg caaattacgc
    28981 gcaaataagc cagcgtcatc agacgaaccg gagcaaccat cactcttttg aacctaatct
    29041 tgcgtatact cctctcatat ctgataagag gaagtgatta tggttcagcg tattactatt
    29101 gcgccgcaag gcccggagtt ttcccgtttt gtgatgggct actggcgatt gatggactgg
    29161 aatatgtccg cccgccagct ggtcagtttt attgaagagc atctggatct cggcgtgacc
    29221 accgtggacc atgctgatat ttatggtggc tatcagtgcg aagcggcgtt tggcgaggca
    29281 ctgaaactgg cacctcacct gcgtgaacgg atggaaatcg tcagtaaatg cggtatcgcg
    29341 acgaccgcgc gtgaagaaaa cgtcattggt cattacatca ctgaccgcga tcacatcatt
    29401 aagagcgccg aacagtcgct aattaatctc gcgaccgatc atctggatct gctgttaatc
    29461 caccgaccag acccgttaat ggatgccgat gaagtggcgg acgcgttcaa acatctgcat
    29521 cagagcggca aagtgcgtca ttttggcgta tcgaacttta cgcctgcgca atttgccctg
    29581 ttgcaatcac gtctgccgtt tacccttgcc actaatcagg tggaaatatc cccggtgcat
    29641 cagccgttac tgctggatgg cacgctcgac caactacaac aactgcgtgt tcgtccgatg
    29701 gcgtggtcct gccttggtgg tggtcgtctg tttaatgatg attatttcca gcagctgcgt
    29761 gatgaactgg ctgtggtggc agaggagtta aacgcgggct cgattgaaca ggtggtttac
    29821 gcctgggtat tacgtttacc atcgcagccg ctgccaatta tcggttcagg taaaattgag
    29881 cgcgtacggg cagctgtcga agcagaaaca ctgaaaatga cccgtcaaca atggtttcgt
    29941 atccgtaaag cggcactggg gtacgacgta ccgtaagcac ttttaggaat agccgccgtt
    30001 caaaaatgtg tcactggttt acacttattc aggaatgcac aatgaacgga ggtcctatga
    30061 aacgttttag tctggctatt ctggcgctgg ttgttgcaac cggcgcacaa gctgccagtg
    30121 aaaaagtcga gatgaacctc gtcacgtcgc aaggggtagg gcagtcaatt ggtagcgtca
    30181 ccattactga aaccgataaa ggtctggagt tttcgcccga tctgaaagca ttaccccccg
    30241 gtgaacatgg cttccatatt catgccaaag gaagctgcca gccagccacc aaagatggca
    30301 aagccagcgc cgcggaatcc gcaggcgggc atcttgatcc acaaaatacc ggtaaacatg
    30361 aagggccaga aggtgccggg catttaggcg atctgcctgc actggtcgtc aataatgacg
    30421 gcaaagctac cgatgccgtc atcgcgcctc gtctgaaatc actggatgaa atcaaagaca
    30481 aagcgctgat ggtccacgtt ggcggcgata atatgtccga tcaacctaaa ccgctgggcg
    30541 gtggcggtga acgctatgcc tgtggtgtaa ttaagtaatt aagaggccag cgtaccttgc
    30601 ggtggtgctt gctcaagttg cgaaagcgag cagtacagcc gccagacaat tgccgccagc
    30661 tcacgggcgg caggttgatg atgacgggca aggctgtcgc aaatccgctg taattcttca
    30721 agtgtggccg ccagtgattt ttgctgaacg ccacgctcac tcatcactcc ccgcaacagt
    30781 gaaatacagt tatcccgtac tcgcgataac ggatcggaac gcgattccca gtcgcgcaat
    30841 tgccagacaa catgagaaca gttcagcagc actacacccc agcgtaataa ccaacggcga
    30901 gccagcgcat cctggctgtt actcaactga ctgacgtgat gataagtgag cgattcaaat
    30961 tcgctttcac tcagtgttgg atggcggctt agctgatcga caaaatcccg gcgcagcgcg
    31021 cgaatatggc ggcggctttt acgagcatcc gatcctggac gcagaatggc gaacgctagc
    31081 caagccagcg caacgccaac gatttttgcc agattatcgt taagaaaatc agcaaaatca
    31141 tacaccgggg gattagtgac agcgataaac gaacccataa aaacaatcag ttgcccccac
    31201 agtgcggcaa atttgggcat ctgcaattta agaagctgca ttgtcgccag cagtggaaag
    31261 agaaacagta aaaattgcca cagatcgcta atctggacca tcaggccaaa tttgaccaca
    31321 aagctgaata gcgaaagtaa caccagcgtg cgcatcagca gtgacaacga cttaaacggt
    31381 gctgcgacgg cggagtagag tacgcagcta attgctgcca gcgttaatgc attggcaccg
    31441 gcatcccatt gcgatgcaat actccatgcg ccaatcatca tcaacgtaca aaatgtacgc
    31501 agcccgctcc acatagcttc ggcgttgtcg gtatgacgag ccagagcgga tgtccgtgcg
    31561 agtctggcat gatcatctac accgctttgc agacgatgta attcctgact actttgcaga
    31621 taaaggcggc aaaaataacg tagtcgctgc cagaaggcga cgtgccggta gtcggcgacg
    31681 ttggtcgggc gtagcggggc gataatacgt gcgacggtgt aagcatctgt ttacgaactg
    31741 gcgagcgccg tcagcaattg ttcgagaatt tctcgcgtgg cacctggcgg tgagggccag
    31801 ttgagcaaca tacgtcgcag gctggagatg acactggtca tacgtaattg ctggtggagt
    31861 agcgcattaa ggcgcgcgtt ttgctggcga aaacgatagt ggctccagaa agcctggata
    31921 cgcagcaaat tcatggtcag tatctgccca atcaccccct catgtgctgc acgaatggca
    31981 tcgtttgttt caggctgcca gagtaaactg gcatgttcca gtaatcgggc gtgcatgttt
    32041 ttcaatgcgg ttaaaagggc tgtagcatcg gaactgctag gcaggatcat catcatcatg
    32101 ccgccgcaca aaataccgac tatcacctcg caaacgcgcg cctgagcgat atcccacagc
    32161 tggctggcct cagtaatatt aaccatcgga aaggcgatga tggcagccgt gtagcccgcc
    32221 agttgaaatg catacgcgac gttattcgtg aagtgcgcac aggcccaggt acaaaagcca
    32281 agccacgccg acatgctcaa tagaaaaaac cacggctcat tgagcgtatg cccggcaaga
    32341 agcagtgccg caatggctcc cagtaaactg ccagcgatgc gtccgaggct tttgctgatg
    32401 acaccgccaa cggtgggaaa gctaaccact gcagccgagg tcatcgccca atagggttca
    32461 tccagattca aataataggc aaccgtcagc gccagacaca tggcaatggt attgcgtaac
    32521 gcataacgcc attgcgccag cgtggccttg aaccagggca aattgcgcaa ggaccatgac
    32581 gatgcgttca ttattgacct acggcaatgc tacaggtggt gcccgacacc agcgtgacat
    32641 cgcctggaac tttatccagc gcaaagcgga cgggaacgcg ttgggcgaga cgaacccagg
    32701 ggacgttagg tttcacatcc ggaataaggc ttgagtcact ttcaacgctt tgatcataaa
    32761 tcgcccgacc gatactggaa acgtgacccc gtaacgtttt gttatcacta tagagggtaa
    32821 tttgtgctgg tgcaccttcg cggatatggc gcaacttggt ttcttcaaaa taaccaatga
    32881 cgtaaaacga gtggctgtcg acaagggcaa acagcggttt cccggtgtca gcgtaatcgc
    32941 cgatgcgggt agtgaggtta gtcacccaac cactcaccgg ggcgcgaatt tccgtttgcg
    33001 ccagttgcca ttgtgtctgt ttcagcgtgg cctgtgcggc attaacactg gcctgcatcg
    33061 ctttaacatt gaggttagcg gtatcgagtt cttcggcaga aataaaattt tgtgagagat
    33121 gacgacgacg attcgcttcg ttattggctt ttgcgaggtc agattgcgct ttcgccagct
    33181 gagcctgtgc gttcagctcg gcgatctgaa agggcgtttt atcgatcgtg agcagaagat
    33241 cccccgcgtt aaccagttgg ttgtctttta tattcagctc aacaatacgt cccgacacct
    33301 gtggggtgat agaaaccttt tctgcgcgta ttttcccgtc gcgtgtccac ggagattgca
    33361 tgtaataatt ccacagccac catccggcaa gaaccgcaac tatcgctaca atgattgttg
    33421 agaaatactt aattgtttta attgacatag ttaccacgca atcagtattg ccagagcaag
    33481 gcaaacgcaa atcgcaaaca gcgataaatc cattaacaag ggatgccaga tgtcaccggc
    33541 gtagatccag ccacgaagca ggcggtgtac gacaagccag atgacgaatc caaacgcgaa
    33601 ggctttgaaa aacggaggaa aatagacgga cgcaccgaac accagatctt gtaagggcaa
    33661 tcctgttgca ttgagcataa acttcacaaa gtagattcct ttacgaccga atatattgcg
    33721 ttagattaat aaatattctt taagtgcgaa aaatttacgc gcaatttctg aaaaatatgt
    33781 ctgacggtaa ccaaatgcag caatacattt gttttagcaa tacaattgct gcacactatt
    33841 ctaaaagccg cataatatct tagcaagcta attataagga gatgaaattg gaatcgccac
    33901 taggttctga tctggcacgg ttggtgcgca tatggcgtgc tctgatagac catcgcctga
    33961 aaccgctgga gttaacacaa acccattggg ttacgttaca caatatccat cagttacctc
    34021 cagaccagtc gcaaattcaa ctggcaaaag cgattggcat cgagcagcca tcactggtcc
    34081 gtactctgga ccaactggaa gaaaaagggt taatttcgcg tcaaacttgt gccagcgatc
    34141 gtcgggctaa acgtattaaa ctgacggaaa aggcagagcc actgatcagc gaaatggaag
    34201 ctgttattaa caaaacccgc gcggaaatat tacatggcat ctccgcagag gaactggagc
    34261 aactgattac gctcatcgca aaacttgagc ataatatcat tgagttacag gccaaagggt
    34321 gaaatgaagg gggcgtgtgg tcagtaacct gaccacacgc aaacttattt agcgcggaga
    34381 aacggtcacc tgactgccat tgctggccag tacgacacgt tggcccggag agaaacgagt
    34441 gttgccttgt ttctgtacca ccatgatggt attaccatcg tctttacgaa tttccagctc
    34501 gacaccctgc gttttgttca ttgcactctg tacgccctga ccagctacgc cacctgcaac
    34561 agcgcctgct gcagtagcca gagaacgccc ggttccgcca ccaacagtat tccccaggaa
    34621 accaccaaga acagcaccgc caattgcacc gataacgttg gaatcatcac cgccctgaat
    34681 ctgtaccgga cgtacgttaa cgatggtgcc atagctgaca ttctgtactt gtttcgcttc
    34741 agaagcggta taaacatccc ctgacagggt gtcgttatta acacaaccga caagagacag
    34801 acctaccatt gaaacaacca atacgcgttt aatcattgaa aaatctcctg ttcaccatga
    34861 aacgccacgc aagcatccct catggtcaaa gtatatggca tatgtgaatc catggttaca
    34921 tgttctacca aatatgagca aatcataatg aaagtaggat caaccaatta taaacaaaac
    34981 attcatgatg attattgatt tgaagataat gttttactac caacggtaaa aatcggtcat
    35041 tccgcatggt attcacgaaa agtttatgtt tgagtggtgc gcaaacgtca gattgaaaaa
    35101 ggatgaacta tgaaatcggg ccgctttatt ggcgttatgt caggcaccag ccttgatggt
    35161 gttgatgttg tgttggcgac aatagatgaa caccgggtcg cacagctggc aagcttgagc
    35221 tggccgatcc cggtgtctct gaaacaggct gtactggata tttgccaggg ccagcagctt
    35281 acactttcgc agtttggaca gcttgatact caactcgggc gactttttgc tgatgcggtc
    35341 aatgccttgc ttaaggaaca aaacctgcag gcgagagata tagttgcgat cggctgtcac
    35401 ggtcaaaccg tctggcatga accgacgggc gtggaaccac acactttaca gattggcgat
    35461 aacaatcaaa ttgtggcacg caccggaatt acggttgtcg gtgattttcg ccgtcgcgat
    35521 attgccttgg gaggacaagg cgcaccgctg gtacctgcgt tccatcatgc cttgctggct
    35581 cacccaaccg agcgacgaat ggtgctcaat attggcggca tcgccaatct gtcactgctc
    35641 attcctgggc agccggttgg cggctacgat accggtcctg gtaacatgct gatggatgcc
    35701 tggatctggc gtcaggccgg taaaccttac gataaagatg ccgagtgggc acgggcgggt
    35761 aaagttattc tcccactgct gcaaaatatg ctcagcgacc catatttctc gcaacctgca
    35821 ccgaaaagca ccggacgcga atactttaac tacggctggc tggagcgcca tttacgccat
    35881 tttccgggtg ttgatccccg tgatgttcag gccacactgg cagaactcac cgccgtgacc
    35941 atttctgaac aagttttgtt gagcggtggc tgcgaacgat tgatggtatg tggtggaggt
    36001 agtcgtaatc cgctactcat ggcgcgtctg gcggcattac tgccaggcac agaagtcacc
    36061 accaccgatg ccgttggcat tagtggcgat gacatggaag cattggcttt cgcctggctt
    36121 gcctggcgga cgctggcggg attaccagga aatctgcctt ccgtcactgg cgcaagccag
    36181 gagacggtac tgggggctat tttccctgct aacccgtgat atcagagtta actgaatttt
    36241 tcttaccgcg tctgccgtta cacttggaac gttagggagg gcgtattgcc ctccagacca
    36301 ggaaagtctt cgggaaatgt ctatgaaaaa actgttactg atctgtttgc cggtgcttct
    36361 caccggctgt agcgcgttca atcaactggt tgagcacatg caaaccgcta cgctggaata
    36421 ccagtgtgat gaaaaaccgt tgacggtcaa actgaataat ccgcgccagg aagtcagttt
    36481 tgtttacgat aatcaactac tgcatctcaa acagggcatt tcagcgtctg gtgcacgtta
    36541 cactgacgga atctatgttt tctggtcgaa aggcgatgaa gcgactgtct ataaacgcga
    36601 ccgcatcgta ttgaataact gtcagttaca aaatccacag cgttgagatt tttccagggg
    36661 cggcgcacaa tagcgtcacc cactgacaat ccgtaaagaa aaccatgtct gataacgacg
    36721 aattgcagca aatcgcgcat ctgcgccgtg aatacaccaa aggcgggtta cgccgtcgcg
    36781 atcttcccgc cgatccatta accctttttg aacgttggct ctctcaggcg tgtgaagcca
    36841 aactggcaga ccctaccgcg atggtggtcg ctaccgtgga tgaacatgct cagccttatc
    36901 aacgcatcgt tttactcaaa cattacgacg aaaaaggcat ggtgttttac accaacctcg
    36961 gtagccgtaa agcacatcaa attgaaaata atccgcgcgt tagcctgctg ttcccgtggc
    37021 atacccttga acgccaggtg atggtgatcg gtaaagcaga acgactttcg actctcgaag
    37081 tgatgaaata ttttcatagc cgcccgcgtg atagccagat tggtgcatgg gtttcgaagc
    37141 agtccagtcg catttctgcc cgcggtatcc ttgaaagtaa attcctggag ctgaagcaga
    37201 agtttcaaca gggcgaagtg ccattgccca gcttttgggg cggttttcgc gtcagccttg
    37261 aacagattga gttctggcag ggcggtgagc atcgcctgca tgaccgcttt ttgtaccagc
    37321 gagaaaatga tgcgtggaag attgatcgtc ttgcaccctg aaaagatgca aaaatcttgc
    37381 tttaatcgct ggtactcctg attctggcac tttattctat gtctctttcg catctggcga
    37441 aaagtcgtgt accggcaaag gtgcagtcgt tatatacatg gagattttga tggcaagcag
    37501 taacttgatt aaacaattgc aagagcgggg gctggtagcc caggtgacgg acgaggaagc
    37561 gttagcagag cgactggcgc aaggcccgat cgcgctctat tgcggcttcg atcctaccgc
    37621 tgacagcttg catttggggc atcttgttcc attgttatgc ctgaaacgct tccagcaggc
    37681 gggccacaag ccggttgcgc tggtaggcgg cgcgacgggt ctgattggcg acccgagctt
    37741 caaagctgcc gagcgtaagc tgaacaccga agaaactgtt caggagtggg tggacaaaat
    37801 ccgtaagcag gttgccccgt tcctcgattt cgactgtgga gaaaactctg ctatcgcggc
    37861 gaacaactat gactggttcg gcaatatgaa tgtgctgacc ttcctgcgcg atattggcaa
    37921 acacttctcc gttaaccaga tgatcaacaa agaagcggtt aagcagcgtc tcaaccgtga
    37981 agatcagggg atttcgttca ctgagttttc ctacaacctg ttgcagggtt atgacttcgc
    38041 ctgtctgaac aaacagtacg gtgtggtgct gcaaattggt ggttctgacc agtggggtaa
    38101 catcacttct ggtatcgacc tgacccgtcg tctgcatcag aatcaggtgt ttggcctgac
    38161 cgttccgctg atcactaaag cagatggcac caaatttggt aaaactgaag gcggcgcagt
    38221 ctggttggat ccgaagaaaa ccagcccgta caaattctac cagttctgga tcaacactgc
    38281 ggatgccgac gtttaccgct tcctgaagtt cttcaccttt atgagcattg aagagatcaa
    38341 cgccctggaa gaagaagata aaaacagcgg taaagcaccg cgcgcccagt atgtactggc
    38401 ggagcaggtg actcgtctgg ttcacggtga agaaggttta caggcggcaa aacgtattac
    38461 cgaatgcctg ttcagcggtt ctttgagtgc gctgagtgaa gcggacttcg aacagctggc
    38521 gcaggacggc gtaccgatgg ttgagatgga aaagggcgca gacctgatgc aggcgctggt
    38581 cgattctgaa ctgcaacctt cccgtggtca ggcgcgtaaa actatcgcct ccaatgccat
    38641 caccattaac ggtgaaaaac agtccgatcc tgaatacttc tttaaagaag aagatcgtct
    38701 gtttggtcgt tttaccttac tgcgtcgcgg caaaaagaat tactgtctga tttgctggaa
    38761 ataatgcatt aagtggaaag ggggagtgag aaatcactcc ccctggtttt tatacaggga
    38821 acatgatgaa aaatattctc gctatccagt ctcacgttgt ttatggtcat gcgggtaaca
    38881 gtgcggcaga gtttcctatg cgccgcctgg gcgcgaacgt ctggccgctg aacaccgttc
    38941 aattttctaa tcacacccaa tacggcaaat ggactggctg cgtgatgccg cccagccatt
    39001 taaccgaaat tgtgcaaggc attgccgcca ttgataaatt acacacctgt gatgccgtgt
    39061 taagtggcta tctgggatcg gcggagcagg gtgaacatat cctcggtatc gtccgtcagg
    39121 tgaaagccgc gaatccgcag gcgaaatatt tttgcgatcc ggtaatgggt catccggaaa
    39181 aaggctgtat cgttgcaccg ggtgtcgcag agtttcatgt gcggcacggt ttgcctgcca
    39241 gcgatatcat tgcgccgaat ctggttgagc tggaaatact ctgtgagcat gcggtaaata
    39301 acgtcgaaga agcggttctg gcagcgcgcg aactcattgc gcaagggcca caaattgtgt
    39361 tggttaaaca cctggcgcga gctggctaca gccgtgaccg ttttgaaatg ctgctggtca
    39421 ccgccgatga agcctggcat atcagccgtc cgctggtgga ttttggtatg cgccagccgg
    39481 taggtgttgg tgatgtgacg agcggtttac tgctggtgaa actgcttcag ggggcaacgc
    39541 tgcaggaggc gctggaacat gtgaccgctg cagtctacga aatcatggtg accaccaaag
    39601 caatgcagga atatgagctg caagtggtgg ctgctcagga tcgtattgcc aaaccagaac
    39661 attacttcag cgcaacaaag ctctgaaata tctcatttcg gcccacaacg acctgtgggc
    39721 cgatggatat tactttaagc cttccgctga cagcgcgtct tgtacttccg gacgttcagc
    39781 catacgttgc ataaatgctg caatgtgctc taacccttcc agattcagtt tcaccgcgta
    39841 tgcccagcgc agaaccgtaa acagataggc atcagcaatt gtaaatcttt gcccgcagat
    39901 ccagtgctca tccttcagtg cctcgttcac atattgcagc ttcttctcca gctgcgcgcg
    39961 aactgtcggt ttgtactctt ccggtgtatc agggcgaaac agaggtgtga aacctttatg
    40021 cagctcggtg gcgatgtaat tcagccattc gatggtttta tagcgggaaa tactgtttac
    40081 cggtgccagc aactggcggt cggggacgct gtcggcaaga tactgcataa tcgctacgcc
    40141 ttccgtcagc aaagtaccgt catccagcag caatgcaggc acctgcccct tagggttaac
    40201 ggcaaagtaa tcgtcaccgt tttcgagacg ttttttcatt aaatccacac tgacgagggt
    40261 aaaatccttt ccgctctcac gcagggtgat atgggaagcg agagagcagg cacccggttt
    40321 gtagaacaat ttcatcggta actccttttg tctgtggttt aaagtatggt agtgcgttgc
    40381 tgaacaaaaa aaaagccgct aacgaaaagt tagcggcttg taaaagagtt tccctgaatt
    40441 ttacgctaca gcagctttcg ccgctttgtc tgcagcgtca tcctgcgtca tacggtgcag
    40501 tttaggcgcg gtcagcagca tcagtactgc aataatggca gtagcgacac caatctgcaa
    40561 gaatacgcga ccataaactt ccagtgacat cagcgggtcg gtaacgttat ccggcacagc
    40621 catcataccc gcaacataac caccaatcag gtttgcaccg gcagtggtca ggaaccagct
    40681 acccataatg aagcccatca gacgctgcgg aacgagttgt gcaaccattg ccagacccag
    40741 accagagatc atcagttccc cgatgctctg caggccatag cttgcgacca gccagcttac
    40801 agacacgata ccagcgtcag acgcgaattt cgcacccagc ggcagaatca ggaacgcacc
    40861 agaacacatc accatgccga ttgcaaactt ggttggcatc ggcagggtat cgcccatctt
    40921 gttatagata gcggccagaa tcggactacc gatgatgatc cagaacgggt tcagtgcctg
    40981 atactgctca ggttctacgg ccagacccag aatggagtgc tcaacgttac gaatcgcaaa
    41041 gaagttcagt gatgtcggca tctggctgta cagcacgaag aagatgattg cttcgagcat
    41101 caggatgaag gcaacgatca ttttacgacg cgcagcacct ttcatcgcga aggcttcttt
    41161 accgaagata accacgatac cgaaggcaac aacgcccagc gccatacgcg caacttcctg
    41221 attgtgcagc agccaggtgg cgatagcgat cagtgccaca acaccaataa tggttagcag
    41281 caggttacgg tagttgatag gctcgaagtc tggttttgaa ccgtactgtt taacccagcg
    41341 ttggcagaag gcgaagttaa cgatagtgat cagcaggcct acaacgctca atgcaaacgc
    41401 aacgctccag ccgtatttcg cggccagcca cggcgtagca atcatagaga agaaagagcc
    41461 gatgttgacg gacatgtagt acatggtgaa tgcaccgtcc agacgcgggt cgtttttctc
    41521 atagcatgta gaaagcagag aagacgggtt agccttaaac aggccgttac cgaccgcaat
    41581 agccgccata cccatataaa caataccggc gtcgtgacca gaccaggcaa ccagagcata
    41641 accaatcgcc agcacaatag cgccgagcat aattacgcgt ttagtaccca gtaccttgtc
    41701 acctaaccag ccgccgatag cgaccagacc ataaaccagg gcactaaagg aagagaaaag
    41761 ggtgattgaa tccgcttcag acatacccag ttgtttaacc aggtaaacag ccataattcc
    41821 ttgtaggccg taataaccaa aacgttccca taactcaatc gagaagatga gatagaacgc
    41881 cttcggttgt ttgaaagcgt tcaaactgac gctttcagtt ggtttttggt ttgcagtgga
    41941 cacttttacc tcttttttta catcccatat taacgggggt gctttctcgc gttatgtcta
    42001 aaaaacatac gcgcaattgt tatagtggga ggggaaacgg caggtaatgt tcactatctt
    42061 gcactttcag acaagtcctt tgtaataatc tgttacatat aactgaggcg gggaattcaa
    42121 ccattccggg aaatgttatc cagcgttaaa tttttcaaaa aacgtaacgg cagatttttt
    42181 cactgcactg agaggggggt gatggcttaa cggcgatatg ttctgctatt tagcgttttt
    42241 actagtgaga tagtccagtt tctgaaaaat agccagtgta atgttttgta ggtcaatatt
    42301 tgttgcgtgt gtaaatttaa atgattttaa cctgattttt gcaatgtttt tacattgcca
    42361 cagcatcgtg atcttgatca cgaatgaata gaaaatttcg ctattaaaaa aacaattaac
    42421 ctgcattttt ggttatccgc aaacggatta cctgatgata acgctaagca atggcatcat
    42481 tcacgttaaa aagatgtttt aaaagaggat aaagaaaggt tatcaatggg gtaatcggtg
    42541 ttaccccttt tcttcagatg tcaactttct ctttgtattc acaaagatct tcaataatac
    42601 aagagccaca gcggggcttg cgggcaatgc aggtataacg cccgtgcagg atcaaccaat
    42661 ggtggcagtc gactttaaac tctgccggaa ccacttttag tagcttttct tctacctgtt
    42721 cgacattttt ccccggcgca aattgagtac gattacaaac gcggaaaatg tgcgtgtcga
    42781 cagcaatagt cggccagccg aatgcagtgt ttaatacgac gttggctgtt ttacgaccta
    42841 cgccgggcag ggcttcaagc gcagcacgat cttccggaac caagccatta tgctgctcca
    42901 gtaagatacg gcaggttttg atgatatttt ctgctttgct gttataaagc ccaatcgttt
    42961 tgatataggt tttaacccct tcaacgccca gttcaagcat cgctgcaggc gtattcgcca
    43021 ccgggtagag tttcgccgtc gccttattaa cactgacatc ggtcgcctga gcggaaagca
    43081 gtacggcaat cagcaattca aaaggcgaac tgaaattaag ctcggtggtg ggatgaggat
    43141 tgttctcacg caggcgagtg aggatctcca ggcgttttgc tttgttcatc agacattccc
    43201 tgtttcaccg tttggcaatg cacgttcggc agctgcttca gtacggcgct ttttcattct
    43261 ttcatcaatc aggtattttc ctgccagcat cagtcccagg ccaataaatg cacctggagg
    43321 cagcatcgcc agcaggaaag gggagtcggt gtggaaaatt tccacgcgta atacttttgc
    43381 ccagctacct aacagcgcat ctgcaccgtc aaacaatgtg ccattgccga taatttcgcg
    43441 tagtgaaccc agcacgaaca tggcgcaggt tgcgcccata ccaattgaaa agccgtccag
    43501 tgccgaaagc gccggacctt ttttggcggc gaaggcttca gcgcggccca caacgataca
    43561 gttagtgaca atcagcggaa taaaaatccc taatgattga tacaggccaa aggcgtaggc
    43621 gttgatcagc atctgtacag cgctgaccac cgaggcgatg atcatcacgt aaatgggaat
    43681 gcggatctcg gctggcgtcc agtgacgcag cgtcgaaatg gtcaggttgg tcagcgtcag
    43741 taccagcgta gtcgcaagtc ctaaacccag agcgttagtg gcagtggacg tgaccgccaa
    43801 cagaggacaa aggccgagca actggaccag cgcagagttg tttttccaca acccctgaac
    43861 aataacgtct ttaatttcgc tcacgcttta ttctccacag gcaggaagtt gagaaagttg
    43921 tgccggtaac gtctgagcgt acaatccggc gcgttttacc gcattaacca ccgcgcgggg
    43981 agtaatcgtc gcgccggtga actggtcgaa atcaccacca tctttcttca ccgcccagtg
    44041 cgcatcatct gcaccactga tttttttacc cgcaaaatgg gtgatccagt cagaaaggcg
    44101 cagttcgatt ttatcgccaa gccctggcgt ttcgtggtgc tctgtcacgc gcgtgccaag
    44161 taccgtgccg ttaaaatcgg ctcccaccag cagctgaatc gcaccggaat agccatctgg
    44221 cgcggttgct tccagaacgg cggctaccgg tttgtcatcc tgtttggcga tgtaaacccg
    44281 atgctcacct ttacctaact ctggcgcagt taccagatag caactctgtg ccagcgcatt
    44341 gttatagcgt tcggctggca gcacctgatc aaataatgcc ttttgttgca gactggcctg
    44401 ttcagcaatc gtcgttttgg tcatctggtt gatggccgca gttaaccctg ttgaacccgc
    44461 tgcaaatagc gctaacgtaa tgccgtgttt tcggatagtt ttcagcatgg tttacccttt
    44521 gcgatggccg tagacgcgcg gacgcgtgta gtaatcgatc agaggaaccg tgatgttcgc
    44581 cagcaggacg gcaaaagcca cgccgtcagg atagccgccg aaactgcgga tcaaccagac
    44641 taataagccc gccagcgcgc cgaaaataag acgaccacga ttggtcgtag aagcggtaac
    44701 cgggtcagtc aaaataaaga atgcgccgag catggtcgca ccagacagca gatgaatttg
    44761 cggtgctgcc agtgtttctg gtgagaacaa ccagcccaac gttgcgcata acgccagcgt
    44821 tactaagaag ctgaggggaa tatgccagcg aatcgctttc tgccatagca accacaggcc
    44881 gccagccagc caggcgagat ttacccattg ccaaccagcg cccgccagaa taccgctgta
    44941 gatcggatat tgcataatct gttcaaccga atgaccggca cggacagagg ttttaaatgt
    45001 atccagcggt gtcgcctgac taatgccatc aatacctaag cgtagtgtgt tcatatcacc
    45061 accactggcg gtatgtccgc tgaaaataac ctgtatggcg tcgataaaac cagggatgtt
    45121 gaccgcaatt tcatgtggcg gtaaccagct ggtcatctgc accgggaagg agatcagtaa
    45181 gaccacataa ccaatcattg ccggattaaa cgggttttgc cccagaccgc catacaactg
    45241 tttagcgatg atcaccgcaa acaccgtacc cagcacgacc atccaccatg gcgcgagggg
    45301 gggaatactt accgccagca ataagcctgt cagcaatgct gagttatctt tcaacgttgc
    45361 ggctaccgac tgcttgcgta gtttgagtac gagagcttcg gctaacagag cactaaccga
    45421 tgccaacagg atctgaacga gagtacccca accaaaaaac cacagttgcg ctgcgattcc
    45481 tggcacggct gcgagcaaca ccaacagcat aatgcgcgat gtctggcgct ggttatgggt
    45541 ataaggggag ctagctattc tgaataccat ttagtcctcg tttacaacct tctgctgggc
    45601 ggcttttttg gcctgagcgc gggcaatagc cgcggcaact gccgctttgc gcggatcaac
    45661 ctgttcttct ggttctgcgt tagtctgttg ctgttccagc ttgcgcgctt tggcacgggc
    45721 aatagcggct tcgacggcgg ctttgcgcgg atcaacctgt tcttctggtt cc
//
LOCUS       DAZGLJ010000012        45454 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000012 DAZGLJ010000000
VERSION     DAZGLJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.012"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            124..1488
                     /gene="yajR"
                     /locus_tag="ACHK49_000613"
     CDS             124..1488
                     /gene="yajR"
                     /locus_tag="ACHK49_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9261305.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(1616..2107)
                     /gene="yajQ"
                     /locus_tag="ACHK49_000614"
     CDS             complement(1616..2107)
                     /gene="yajQ"
                     /locus_tag="ACHK49_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO9261306.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2275..3186
                     /gene="panE"
                     /locus_tag="ACHK49_000615"
     CDS             2275..3186
                     /gene="panE"
                     /locus_tag="ACHK49_000615"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO9261307.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3149..3739
                     /gene="yajL"
                     /locus_tag="ACHK49_000616"
     CDS             3149..3739
                     /gene="yajL"
                     /locus_tag="ACHK49_000616"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO9261308.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3793..5241)
                     /gene="thiI"
                     /locus_tag="ACHK49_000617"
     CDS             complement(3793..5241)
                     /gene="thiI"
                     /locus_tag="ACHK49_000617"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO9261309.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5447..5689
                     /gene="xseB"
                     /locus_tag="ACHK49_000618"
     CDS             5447..5689
                     /gene="xseB"
                     /locus_tag="ACHK49_000618"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO9261310.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5689..6588
                     /gene="ispA"
                     /locus_tag="ACHK49_000619"
     CDS             5689..6588
                     /gene="ispA"
                     /locus_tag="ACHK49_000619"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO9261311.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6613..8475
                     /gene="dxs"
                     /locus_tag="ACHK49_000620"
     CDS             6613..8475
                     /gene="dxs"
                     /locus_tag="ACHK49_000620"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO9261312.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8655..9629
                     /gene="yajO"
                     /locus_tag="ACHK49_000621"
     CDS             8655..9629
                     /gene="yajO"
                     /locus_tag="ACHK49_000621"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO9261313.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFVSMQDHYNLIYREEESEMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9683..10201)
                     /gene="pgpA"
                     /locus_tag="ACHK49_000622"
     CDS             complement(9683..10201)
                     /gene="pgpA"
                     /locus_tag="ACHK49_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO9261314.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10179..11156)
                     /gene="thiL"
                     /locus_tag="ACHK49_000623"
     CDS             complement(10179..11156)
                     /gene="thiL"
                     /locus_tag="ACHK49_000623"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO9261315.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11234..11653)
                     /gene="nusB"
                     /locus_tag="ACHK49_000624"
     CDS             complement(11234..11653)
                     /gene="nusB"
                     /locus_tag="ACHK49_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO9261316.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11673..12143)
                     /gene="ribE"
                     /locus_tag="ACHK49_000625"
     CDS             complement(11673..12143)
                     /gene="ribE"
                     /locus_tag="ACHK49_000625"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO9261317.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12232..13335)
                     /gene="ribD"
                     /locus_tag="ACHK49_000626"
     CDS             complement(12232..13335)
                     /gene="ribD"
                     /locus_tag="ACHK49_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO9261318.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13339..13788)
                     /gene="nrdR"
                     /locus_tag="ACHK49_000627"
     CDS             complement(13339..13788)
                     /gene="nrdR"
                     /locus_tag="ACHK49_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO9261319.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13939..14478
                     /gene="yajI"
                     /locus_tag="ACHK49_000628"
     CDS             13939..14478
                     /gene="yajI"
                     /locus_tag="ACHK49_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO9261320.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            14777..15661
                     /gene="tsx"
                     /locus_tag="ACHK49_000629"
     CDS             14777..15661
                     /gene="tsx"
                     /locus_tag="ACHK49_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO9261321.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15838..16185)
                     /gene="yajD"
                     /locus_tag="ACHK49_000630"
     CDS             complement(15838..16185)
                     /gene="yajD"
                     /locus_tag="ACHK49_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO9261322.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16314..17285)
                     /gene="secF"
                     /locus_tag="ACHK49_000631"
     CDS             complement(16314..17285)
                     /gene="secF"
                     /locus_tag="ACHK49_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO9261323.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17296..19143)
                     /gene="secD"
                     /locus_tag="ACHK49_000632"
     CDS             complement(17296..19143)
                     /gene="secD"
                     /locus_tag="ACHK49_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO9261324.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19171..19503)
                     /gene="yajC"
                     /locus_tag="ACHK49_000633"
     CDS             complement(19171..19503)
                     /gene="yajC"
                     /locus_tag="ACHK49_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO9261325.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19526..20653)
                     /gene="tgt"
                     /locus_tag="ACHK49_000634"
     CDS             complement(19526..20653)
                     /gene="tgt"
                     /locus_tag="ACHK49_000634"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO9261326.1"
                     /translation="MKFELDTTDGRARRGRLVFDCGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20709..21779)
                     /gene="queA"
                     /locus_tag="ACHK49_000635"
     CDS             complement(20709..21779)
                     /gene="queA"
                     /locus_tag="ACHK49_000635"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO9261327.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21872..22453
                     /gene="acpH"
                     /locus_tag="ACHK49_000636"
     CDS             21872..22453
                     /gene="acpH"
                     /locus_tag="ACHK49_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO9261328.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22458..24275)
                     /gene="malZ"
                     /locus_tag="ACHK49_000637"
     CDS             complement(22458..24275)
                     /gene="malZ"
                     /locus_tag="ACHK49_000637"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO9261329.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFDGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(24431..25804)
                     /gene="proY"
                     /locus_tag="ACHK49_000638"
     CDS             complement(24431..25804)
                     /gene="proY"
                     /locus_tag="ACHK49_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO9261330.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25880..27199)
                     /gene="brnQ"
                     /locus_tag="ACHK49_000639"
     CDS             complement(25880..27199)
                     /gene="brnQ"
                     /locus_tag="ACHK49_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO9261331.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27606..28901)
                     /gene="phoR"
                     /locus_tag="ACHK49_000640"
     CDS             complement(27606..28901)
                     /gene="phoR"
                     /locus_tag="ACHK49_000640"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO9261332.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWLRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28959..29648)
                     /gene="phoB"
                     /locus_tag="ACHK49_000641"
     CDS             complement(28959..29648)
                     /gene="phoB"
                     /locus_tag="ACHK49_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO9261333.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29838..31040
                     /gene="sbcD"
                     /locus_tag="ACHK49_000642"
     CDS             29838..31040
                     /gene="sbcD"
                     /locus_tag="ACHK49_000642"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO9261334.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            31037..34180
                     /gene="sbcC"
                     /locus_tag="ACHK49_000643"
     CDS             31037..34180
                     /gene="sbcC"
                     /locus_tag="ACHK49_000643"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO9261335.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRQDELQQEASRRQQALQQVLAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQRIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASETQEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34306..35490
                     /gene="araJ"
                     /locus_tag="ACHK49_000644"
     CDS             34306..35490
                     /gene="araJ"
                     /locus_tag="ACHK49_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO9261336.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCCGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35633..36541)
                     /gene="mak"
                     /locus_tag="ACHK49_000645"
     CDS             complement(35633..36541)
                     /gene="mak"
                     /locus_tag="ACHK49_000645"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO9261337.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36666..37577
                     /gene="rdgC"
                     /locus_tag="ACHK49_000646"
     CDS             36666..37577
                     /gene="rdgC"
                     /locus_tag="ACHK49_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO9261338.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37655..37738)
                     /locus_tag="ACHK49_000647"
     CDS             complement(37655..37738)
                     /locus_tag="ACHK49_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9261339.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(37735..38076)
                     /locus_tag="ACHK49_000648"
                     /pseudo
     CDS             complement(37735..38076)
                     /locus_tag="ACHK49_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(38224..38508)
                     /gene="ppnP"
                     /locus_tag="ACHK49_000649"
     CDS             complement(38224..38508)
                     /gene="ppnP"
                     /locus_tag="ACHK49_000649"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO9261340.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(38580..39257)
                     /gene="aroM"
                     /locus_tag="ACHK49_000650"
     CDS             complement(38580..39257)
                     /gene="aroM"
                     /locus_tag="ACHK49_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO9261341.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(39515..39706)
                     /gene="yaiA"
                     /locus_tag="ACHK49_000651"
     CDS             complement(39515..39706)
                     /gene="yaiA"
                     /locus_tag="ACHK49_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO9261342.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(39756..40280)
                     /gene="aroL"
                     /locus_tag="ACHK49_000652"
     CDS             complement(39756..40280)
                     /gene="aroL"
                     /locus_tag="ACHK49_000652"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO9261343.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(40463..40921)
                     /gene="yaiI"
                     /locus_tag="ACHK49_000653"
     CDS             complement(40463..40921)
                     /gene="yaiI"
                     /locus_tag="ACHK49_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO9261344.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            41041..41850
                     /gene="proC"
                     /locus_tag="ACHK49_000654"
     CDS             41041..41850
                     /gene="proC"
                     /locus_tag="ACHK49_000654"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO9261345.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(41867..42982)
                     /gene="adrA"
                     /locus_tag="ACHK49_000655"
                     /gene_synonym="dgcC"
     CDS             complement(41867..42982)
                     /gene="adrA"
                     /locus_tag="ACHK49_000655"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO9261346.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(43084..43404)
                     /gene="psiF"
                     /locus_tag="ACHK49_000656"
     CDS             complement(43084..43404)
                     /gene="psiF"
                     /locus_tag="ACHK49_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO9261347.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(43523..44938)
                     /gene="phoA"
                     /locus_tag="ACHK49_000657"
     CDS             complement(43523..44938)
                     /gene="phoA"
                     /locus_tag="ACHK49_000657"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO9261348.1"
                     /translation="MKQSTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITASGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(45039..45299)
                     /gene="iraP"
                     /locus_tag="ACHK49_000658"
     CDS             complement(45039..45299)
                     /gene="iraP"
                     /locus_tag="ACHK49_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO9261349.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT        11203 a        12181 c        11454 g        10616 t
ORIGIN      
        1 gaatgcaaaa aaacatacct gacacacaaa ttactctcct ctgtaatatt ttctcattaa
       61 cactctgttt acccaacatg cgctccgcac tacactatgt gggcattttt ttaaggggtg
      121 gtaatgaacg attataaaat gacgccaggt gagaggcgcg cgacctgggg tttagggacc
      181 gtattctcgt tgcgcatgct gggcatgttt atggttctgc cggttctgac cacgtacggc
      241 atggctctgc aaggtgccag cgaagcatta atcggtattg ccattggtat ttatggtctg
      301 actcaggccg tttttcagat tccgtttggc ctgctttccg accgcattgg tcgcaaacca
      361 ttaattgtcg gtgggctggc ggtgtttgcc gccggtagcg ttatcgctgc gctttccgac
      421 tccatctggg gaattattct gggccgggcg ctacaaggct ctggagcaat tgccgccgcc
      481 gttatggcgc tgctttccga tctcacgcgc gaacaaaacc gcaccaaagc gatggcgttt
      541 atcggcgtga gctttggcat tacctttgcc attgcgatgg tgcttggccc gatcatcact
      601 cacaaacttg ggctgcacgc gctgttctgg atgatcgcta ttctggcaac aaccggcatt
      661 gcgttgacca tttgggttgt acccaacagt agcactcacg tacttaatcg tgagtccgga
      721 atggtgaaag gcagtttcag taaagtgctg gcggaaccgc ggctgctaaa actcaacttt
      781 ggcattatgt gtctgcacat gcttctgatg tctacgtttg ttgccctgcc cggacagcta
      841 gctgatgcgg ggttcccggc ggctgaacac tggaaggtct atctggcgac gatgttaatc
      901 gcctttggct cggtcgtgcc tttcattatc tacgctgaag ttaagcgcaa aatgaagcaa
      961 gtctttgtct tctgcgtcgg gttgatcgtg gttgcggaaa ttgtgttgtg gaacgcccaa
     1021 acgcagttct ggcaactggt ggtcggcgtg cagcttttct ttgtggcgtt taatttgatg
     1081 gaagcccttc tgccctcact tatcagtaaa gagtcgccag caggttacaa aggtacggcg
     1141 atgggtgtct actccaccag ccagtttctt ggcgtggcga ttggcggttc gctgggcggc
     1201 tggattgacg gcatgtttga cggtcagggg gtatttctcg ctggcgcaat gctggccgca
     1261 gtgtggctgg cagtcgccag taccatgaaa gaaccgccgt atgtcagcag tttgcgcatt
     1321 gaaatcccgg cggacattgc cgcaaacgaa gcgttaaaag tgcgtttgct ggaaactgaa
     1381 ggcgtcaaag aagtgttgat tgcagaagaa gaacattcag cttatgtgaa aatcgacagc
     1441 aaagtgacga atcgctttga gatagaacag gcaattcgtc aggcataaaa aacgcggcga
     1501 attgcctgct gcgctacgct tatcaggcct acaaagtttc tgaattacaa acctttgtag
     1561 gccgaatcag gcgttcatgc cgcatctgac atgaacaaaa cgcacatagt cgcgattaat
     1621 cgcggaagtt tttgaactgg aacggctgac cgagatcgcc accacgtacc atcgccatga
     1681 cagcctgcaa atcatcacga gatttgcccg ttacgcggat ttcatcgccc tgaatttgcg
     1741 cctgcacttt cagtttgctg tctttgatca tcttgacgat tttcttctgg gtcgcgctct
     1801 caatgccctg tttcagtttc gcttccacaa accaggtttt accgctatga acgatattct
     1861 ccggtacatc cagcgaactg ccttcaatgc cgcgcttcag cagcttggca cgcagaatat
     1921 ccagcaactg attgacctgg aaatcggact cgctcaacac tttgatggtt ttgctggcgt
     1981 cgttcagctc aaatgaggct tcaacgttac ggaagtcaaa acgggactcc acttcgcggc
     2041 tcgcgttatc gaccgcgtta cgtgcttcct gaagatcaac ttcagagaca atatcgaaag
     2101 atggcatctt ttcctctccc ttcatttttg atgcgaagca taatacccgc aaagttaaat
     2161 aaccgacagt cctgcaccag gacgtatccc ttaattgtgc atagtcgcaa cagcatgcac
     2221 aattaaggga tagccttatt gaacacccgg agtggttgcg ggtgaggagg aacaatgaaa
     2281 attaccgtat tgggatgcgg tgccttaggg caattatggc ttacagcact ttgcaaacag
     2341 ggtcatgaag ttcagggctg gctgcgcgta ccgcaacctt attgtagcgt gaatctggtt
     2401 gagacagatg gttcgatatt taacgaatcg ctgaccgcca acgatcccga ttttcttgcc
     2461 accagcgatc tgctcctggt gacgctgaaa gcatggcagg tttacgatgc cgtcaaaagc
     2521 ctcgcgtcca cactgcctgt aaccacgcca atactgttaa ttcacaacgg catgggcacc
     2581 atcgaagagt tgcaaaacat tcagcagcca ttactgatgg gcaccaccac ccatgccgcc
     2641 cgccgcgacg gcaatgtcat tattcatgtg gcaaacggaa tcacgcatat tggtccggca
     2701 cggcaacagg atggcgatta cagttatctg gcggatattt tgcaaaccgt gttgcctgac
     2761 gttgcctggc ataacaatat tcgcgccgag ctgtggcgca agctggccgt caactgtgtg
     2821 attaatccac tgaccgccat ctggaattgc ccgaatggtg aattacgtca tcatccgcaa
     2881 gaaattatgc agatatgcga agaagtcgcg gcagtgatcg agcgcgaagg gcatcatact
     2941 tcagcagaag atttgcgtga ttacgtgatg caggtgattg atgccacagc ggaaaatatc
     3001 tcgtcgatgt tgcaggatat ccgcgcgctg cgccacaccg aaatcgacta tatcaatggt
     3061 tttctcttac gccgcgcccg cgcgcatggg attgccgtac cggaaaacac ccgcctgttt
     3121 gaaatggtaa aaagaaagga gagtgaatat gagcgcatcg gcactggttt gcctcgcccc
     3181 tggtagtgaa gagactgaag ccgtcaccac tatcgatctg ctggttcgcg gcggtatcaa
     3241 agtcaccact gccagcgtcg ccagcgatgg caacctggcc attacctgct cgcgcggcgt
     3301 gaagctgctg gcggatgcgc cgctggtcga ggtggctgat ggcgaatatg acgtgatcgt
     3361 actgcctggt ggcattaaag gcgcagagtg ttttcgcgat agtaccctgc tggttgaaac
     3421 cgttaaacag ttccaccgtt ccgggcgtat cgtcgcggct atttgcgccg cgccagccac
     3481 cgtgctggtg ccgcacgata tcttcccgat tggtaatatg accggcttcc cgacgctgaa
     3541 agacaaaatt cccgctgaac aatggcagga caagcgcgtc gtctgggatg cacgggtaaa
     3601 attgctgacc agccaggggc cggggacagc tatcgacttt ggtctgaaaa ttatcgacct
     3661 gctggttggg cgcgagaaag cccatgaagt ggcatcacaa ctggtgatgg cggcagggat
     3721 ttataattat tacgagtagt gtcggatgcg gtaagcgtcg catctgacca gatgcgacgt
     3781 tacaaccaca aattacgggc gatatacctt cacattgtta aagccctgct cgcgcagata
     3841 gagcgcctgc agacggctca tcaccccgcg ctcacaccac agtagccagg ttctgttctg
     3901 gtcgagatcg ccaaacttgg tgctcagttt atagaacggc agagaaacta catcaatccc
     3961 ttcgactttc agtggcttat cttcctgttc atcgatagaa cggatatcga ggatcacgtc
     4021 gttcgggccg aagccattga cggtttccac ttccaccact tcctgctcgg tctgctgggc
     4081 gatttcgcgg atatcaacgt tattcgcttc ttcaaccact ttatcgagaa tgctgaagtc
     4141 gaatttctct tcttccgctt caatcttcga cttaaccgct ttcaccgtcg ggcttttgga
     4201 gataacaccg caatattccg gcatcgtgcg agcaaagtct tcggtgccaa tctggcgagc
     4261 caggttgatg atgtgctctt tgtcgtaaga aatcagcgga cgcaggatca gcgtatcgga
     4321 gacgttatca atcaggcgca ggttggtcag cgtctgactg gacacctggc cgagcgcttc
     4381 gccggtgacc agcgcctgta cgccgtaacg ttcagccact ttagacgcgg cacgcaccat
     4441 catacgtttg aggataacgc ccatctgacc gtcgtcgatt ttctcgagaa tttccccgac
     4501 gaccggttcg aaattaatag cgacaaaacg cacgcggtgg gagctgccaa aacggttcca
     4561 cagataatgc gccacctgac gcacgccaat ttcatgcgcc gcgccgccaa ggttaaagaa
     4621 acagtaatgc acgcggcagc cgcgacgcat caacatataa ctggaaacac cggagtcgaa
     4681 accaccggaa atgagcgaca gcacatcttc ctgggtgccg atcgggaaac cgccaatacc
     4741 ttcgtagcgg cctttaatca gcaggagacg atcgtcttcc acttccagat ggacagtcac
     4801 ttccggattg gtcagcttca cgcgcgcgga ctcaatatgc tgatttaaac cgccgccgac
     4861 gtaacgttcc acatcaatcg agctaaaatc atgtttgcca cggcgcttca cgcgtacgca
     4921 gaaggttttg ccttccagct gatcgcgata ctgaaccaac gctttctcaa aaatatcgtg
     4981 catgtcggta aacggcacgt cttcgacttc gagaatatgg tggatacccg gaatacgggt
     5041 cagggcgtcg cgaatagcca gacgctggtt ttcatctttt gcgcgaactt cgatgttatc
     5101 ccagtggcgg acgacagcga gcgtctcatc atagtgcttt aaaacgttac gaatgttccc
     5161 ggtaaggatt tttataaagc gcaagcgcac agattggctt ttgatggtga tttccgggaa
     5221 caatttaatg ataaacttca tggcggcaat ggttcgttgg caagccttaa gcgacttgta
     5281 tagggaaaaa tacagcagcc cacacctgcg gctgcatcca ggcgcggaag tataccacta
     5341 acatcgcttt gctgcgcaca tcaccttacc attgcgcgtt atttgctatt tgccctgagt
     5401 ccgttaccat gacggggcga aaaatattga gagtcagaca ttcattatgc cgaagaaaaa
     5461 tgaggctccc gccagctttg aaaaggcgct gagcgagctg gaacagattg taacccgtct
     5521 ggaaagtggc gacctgccgc tggaagaggc gctgaacgag ttcgaacgcg gcgtgcagct
     5581 ggcacgtcag gggcaggcca aattacaaca agccgaacag cgcgtacaaa ttctgctgtc
     5641 tgacaatgaa gacgcctctc taaccccttt tacaccggac aatgagtaat ggactttccg
     5701 cagcaactcg aagcctgcgt taagcaggcc aaccaggcgc tgagccgttt tatcgcccca
     5761 ctgccctttc agaacactcc cgtggtcgaa accatgcagt atggcgcatt attaggtggt
     5821 aagcgtctgc gacccttcct ggtttatgcc accggtcata tgtttggcgt tagcacaaac
     5881 acgctggacg cacccgctgc cgccgtagag tgtatccacg cttactcatt aattcatgat
     5941 gatttaccgg cgatggatga tgacgatctg cgtcgcggtt tgccgacctg ccatgtgaag
     6001 tttggcgaag caaacgcgat tctcgctggc gacgctttac aaacgctggc gttctcgatt
     6061 ctaagcgatg ccaatatgcc ggaagtgtcg gatcgcgaca gaatttcgat gatttctgaa
     6121 ctggcgagcg ccagcggtat tgccggaatg tgcggtggtc aggcattaga tttagacgcg
     6181 gaaggcaaac acgtacctct ggacgcgctt gagcgtattc atcgtcataa aaccggcgca
     6241 ttgattcgcg ccgccgttcg ccttggtgca ttaagcgccg gagataaagg gcgtcgtgct
     6301 ctgccagtac tcgacaagta cgcagagagc atcggccttg ccttccaggt tcaggatgac
     6361 atcctggatg tggtaggaga tactgcaacg ttgggaaaac gccagggtgc cgaccagcaa
     6421 cttggtaaaa gtacctaccc tgcacttctg ggtcttgagc aagcccggaa gaaagcccgg
     6481 gatctgatcg acgatgcccg tcagtcgctg aaacaactgg ctgaacaatc actcgatacc
     6541 tcggcactgg aagcgctagc ggactacatc atccagcgta ataaataaac aataagtatt
     6601 aataggcccc tgatgagttt tgatattgcc aaatacccga ccctggcact ggtcgactcc
     6661 acccaggagt tacgactgtt gccgaaagag agtttaccga aactctgcga cgaactgcgc
     6721 cgctatttac tcgacagcgt gagccgttcc agcgggcact tcgcctccgg gctgggcacg
     6781 gtcgaactga ccgtggcgct gcactatgtc tacaacaccc cgtttgacca attgatttgg
     6841 gatgtggggc atcaggctta tccgcataaa attttgaccg gacgccgcga caaaatcggc
     6901 accatccgtc agaaaggcgg cctgcacccg ttcccgtggc gcggcgaaag cgaatatgac
     6961 gtattaagcg tcgggcattc atcaacctcc atcagtgccg gaattggtat tgcggttgct
     7021 gccgagaaag aaggcaaaaa tcgccgtacc gtctgtgtaa tcggcgatgg cgcgattacc
     7081 gcaggcatgg cgtttgaagc gatgaatcac gcgggcgata tccgtcctga tatgctggtg
     7141 gtcctcaacg acaacgaaat gtcgatttcc gaaaatgtcg gcgcgctcaa caaccatctg
     7201 gcgcagctgc tttccggtaa gctttactct tcactgcgcg aaggcgggaa aaaagttttc
     7261 tctggcgttc cgccaattaa agagctgctc aaacgtaccg aagaacatat taaaggcatg
     7321 gtagtgcctg gcacgttgtt tgaagagctg ggctttaact acatcggccc ggttgacggt
     7381 cacgatgtgc tggggcttat caccacgctg aagaacatgc gcgacctgaa aggcccgcag
     7441 ttcctgcata tcatgaccaa aaaaggtcgt ggttatgaac cggcagaaaa agaccccatc
     7501 actttccacg ccgtgcctaa atttgatccc tccagcggtt gtttgccgaa aagtagcggc
     7561 ggtttgccga gctattcaaa aatctttggc gactggttgt gcgaaacggc agcgaaagac
     7621 aacaagctga tggcgattac tccggcgatg cgtgaaggtt ccggcatggt cgagttttca
     7681 cgtaaattcc cggatcgtta cttcgacgtg gcaatcgccg agcaacacgc ggtgaccttt
     7741 gccgccggtc tggcgattgg tgggtacaaa cccattgtcg cgatttactc cactttcctg
     7801 caacgcgcct atgatcaggt gctgcatgac gtggcgattc aaaagcttcc ggtcctgttc
     7861 gccatcgacc gcgcgggcat tgttggtgct gacggtcaaa cccatcaggg cgcttttgac
     7921 ctctcttacc tgcgctgcat accggaaatg gtcattatga ccccgagcga tgaaaacgaa
     7981 tgtcgccaga tgctctatac cggctatcac tataacgacg gcccgtccgc ggtgcgctac
     8041 ccgcgcggta acgcggttgg cgtggaactg acgccgctgg aaaaactgcc aattggcaaa
     8101 ggcattgtga agcgtcgtgg cgagaaactg gcgatcctta actttggtac gctgatgcca
     8161 gacgcggcga aagtcgctga atcgctgaac gctacgctgg tcgatatgcg ttttgtgaaa
     8221 ccgcttgatg aagcgttaat tctggaaatg gccgccagcc atgaagcgct ggtcaccgta
     8281 gaagaaaacg ccattatggg cggcgcaggc agcggcgtga acgaagtgct aatggcccat
     8341 cgtaaaccag tacccgtgct gaacattggc ctgcctgact tctttattcc acaaggaact
     8401 caggaagaaa tgcgcgccga actcggcctc gatgccgccg gtatggaagc caaaatcaag
     8461 gcctggctgg cataatccct actccactcc tgctatgctt aagaaattat tcatagactc
     8521 taaataattc gagttgcagg aaggcggcaa acgagtgaag ccccaggagc ttacataagt
     8581 aagtgactgg ggtgaacgaa tgcagccgca gcacatgcaa cttgaagtat gacgagtata
     8641 gcgggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac
     8701 tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg taatcacgca tggacactgc
     8761 cggaagagag cagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg
     8821 ataccgccaa tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg
     8881 atttcgcccg tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt
     8941 taccggaagg attatcccgt gcacaaattt tgcgctctat cgacgacagc ctgcgccgtc
     9001 tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg
     9061 aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg
     9121 cgtcatccat gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct
     9181 gggcgcagtt tgtcagtatg caggatcact acaatctgat ttatcgcgaa gaagagagcg
     9241 agatgctgcc actgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc
     9301 gggggcgact gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg
     9361 ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacag
     9421 gcgtcagcga agaactcggt gcaacacgag cacaagttgc gctggcctgg ttgttgagta
     9481 aaccgggcat tgccgcaccg attatcggaa cttcgcgcga agaacagctt gatgagctat
     9541 tgaacgcggt ggatatcact ttgaagccgg aacagattgc cgaactggaa acgccgtata
     9601 aaccgcatgc ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt
     9661 cacatcaggc atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa
     9721 agtacaggat gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc
     9781 cgccatgtac attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac
     9841 ggaaaatcac aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg
     9901 tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat
     9961 ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg
    10021 agtagagctg ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc
    10081 ccatcgtgcc aggaacgatt gggcttaatc cacttccgaa tccgacagca agcagatgcc
    10141 acggattact catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca
    10201 tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata
    10261 tcggcggtca tttgcccgat acaggtaaac ggtacgccca ggtgtccgag agccacatcc
    10321 agcgcgccac ggttcagttc cggcacagtg aaacacaact cgtaatcttc accgccagag
    10381 agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc
    10441 agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg
    10501 gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc
    10561 tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca
    10621 tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta
    10681 ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc
    10741 ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca
    10801 atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc
    10861 cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc
    10921 atcgctgcca gatcgcttag gttcactgcc agtgctttat acgccagatc agcaggatcg
    10981 atatcatgga ggaagtggtt gcccgccacc agcgtatcag tgctgatcgc cagggtctgt
    11041 ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac atcaagacga
    11101 gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt
    11161 tatgcctcag caggtaaaaa agaaaaggcc ggcgatcgcg gaacggtctt ccgtgaatct
    11221 accggcctgg atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc
    11281 cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt
    11341 taatggccac tttgtatggc acatcgctac gtttagacag ctcgtacagc gcaatgcgca
    11401 gtactgcttt ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca
    11461 gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg aagtacagga
    11521 cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt
    11581 tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag
    11641 cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta
    11701 atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca
    11761 cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg
    11821 ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg
    11881 gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta
    11941 gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg
    12001 ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg
    12061 tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca
    12121 acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg
    12181 gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc
    12241 cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg
    12301 ggcgtcggct aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa
    12361 tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc
    12421 agcgagcgtt ggccccgctt ccacccagat actgttaatt tgctgtttac ccagttgcat
    12481 catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt
    12541 ttccggccac tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg
    12601 cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg
    12661 gcggagattt tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac
    12721 tgttaaggct ggatcatccg ccagcaccgt ggcgctgcta gttaaaatgg catgactttg
    12781 tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc
    12841 gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa
    12901 gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag
    12961 cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg
    13021 cgggttagga tcttgcatcg aggcaaccac gcgcgctacg ccagcggcga tgagtgcgtc
    13081 acagcacggt ggcgtacgac catgatggct acagggttcg agtgtgacat aggcggtcgc
    13141 acctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc
    13201 acgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc acccgacatt
    13261 cgggttggga tgcgtggtaa aacgtcctcg ttgcgccagc tttagcgctc gcgccatgta
    13321 atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat
    13381 atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt
    13441 caattgctcc atcaccagat tgccaatcat cttgctcggc acttcgcgct caccggtggc
    13501 gcgcagctgc gatttaatat gattgattgc catttcgacg tcatcggaac tcaccggacg
    13561 tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac
    13621 gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg
    13681 ttcattacac accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg
    13741 agagtcaatt accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga
    13801 ccaggtggtt aacagaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc
    13861 ttttgagata aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg
    13921 acgagaaagg gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg
    13981 ttcagcctta gcgcctgtgt gcagcagagc gaagtgcgac agatgaaaca cagcgtcagc
    14041 acgctgaacc aagagatgac gcaactcaat aaagaaacgg tcaaaatcac tcagcaaaac
    14101 aggctgaatg caaaatccag cagtggggtt taccttctgc caggagcgaa aacaccggca
    14161 agactggaaa gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgatgca
    14221 gatggcacca ccctgacgct acgtattcag ggcgagtcga atgatccctt gcccgcattc
    14281 agcggaactg ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat
    14341 gtgcagaatc aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt
    14401 ccgttacagt taaagggtat ttctgtagaa cagttagact tcgtacgtat ccacgatatt
    14461 caacctgtta tgcagtaaac gtatttcggg gcgattttgt gcgtctcgca acatctttcc
    14521 ccgtcatttt gttactccgc ttacatcacc ctgattgata gtaaaagttt gcaacaaggg
    14581 tgaaagtcag tacaatccct gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa
    14641 atgatagaac tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct
    14701 ctgtatacga aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc
    14761 aaacagtggc atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc
    14821 ttcgtctttt actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg
    14881 gcaccagagc gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa
    14941 cgatacctac cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta
    15001 tgcggatgcg ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg
    15061 ttctccgctg tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga
    15121 ccttagcttc ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg
    15181 tcgtaataaa gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac
    15241 tggtctgccg atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatgg
    15301 cgcagcgaac gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat
    15361 taccgatctg tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc
    15421 cgatttaggg gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc
    15481 tatcgcttcc agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc
    15541 tcgttactgg cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg
    15601 caacttcaac gttcgctcta ccggctgggg tggttacctg gttgtaggtt acaacttctg
    15661 attatgaaaa tgccgggatt tattcccggc atttcagatt gttaacaacg tgcgctttgt
    15721 tcatgccgga tgcggcgtaa acgctttgtc cgacccacaa aagcacgtaa attcaatata
    15781 ttgctgaaat aacgtagtcc tgataagcgt agcgcatcag gcaattttac gttttaatca
    15841 cttcttcttg ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac
    15901 atctttctgc gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata
    15961 tttcgaatgc tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc
    16021 cgggttattg gtatggtcgt gatcaatgtg gtgaacagta agttcacgca ggttggaata
    16081 aacaaactcg cgggaacagc gaccgcagac ccacggatat atttttaatg ctttttcgcg
    16141 atagccgctt tctaaccgcg catagttttt tgggatgata gccatgaaga gttacctgga
    16201 tgaaatgtag ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat
    16261 caaaaaaaat cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc
    16321 agaattgacg gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc
    16381 ttcataccca gtttcagagc caacgcagat gccacataga tggaagatgc agtaccgatg
    16441 gaaacaccga taagcatggt cagcgagaag ccttccagta ccggaccacc gaagaggtac
    16501 agcatcagga taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg
    16561 gtcaaggaca cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca
    16621 cgaatacggt ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac
    16681 gatgccacaa tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg
    16741 ataacgtcgt gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcgaaaa
    16801 cctacgtaca caaggataga cagcagcgcc gccatcaacg ccatcgcacc ggtttgcgca
    16861 aggtctgccc ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg
    16921 gtggattcgt taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct
    16981 tcagcaggcg gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc
    17041 tcttcaaaac cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg
    17101 agcgtaattt cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg
    17161 cccataataa cgatagcggc gattaacagc agaccagaga tgccgaaagc ccagtagtcc
    17221 cagcgcataa agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt
    17281 gccacatcgc actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt
    17341 tacgatggca cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt
    17401 agtaatcgcg aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat
    17461 cagcgtggtg atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat
    17521 tgcctgttga acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac
    17581 gttcgcatcg accgccaccg caagggttaa gacgataccc gcgatacctg gcatactcag
    17641 cgttgcgcct ggcaacagcg acataatgcc gacgattaag atcaagttgg caatcagagc
    17701 actggtcgca atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag
    17761 cagaccggca aggcaagcct ccagcccctg ttcaatgttc tgcataccca gggttgggcc
    17821 aatggtacgt tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag
    17881 tgacagctga cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag
    17941 acgagactgg atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc
    18001 atttgcatct ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc
    18061 gatgttgtcc ttagtgaagt tagacatgat gttaccacca gcgctatcga gcgagatgtt
    18121 aacctgcggt tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt
    18181 cagaattacg cgtttgtaca gcacaactgg ctgaccttcg cgggtctgtt tcacttcaga
    18241 gtcgcccggt acgcgaccgg atgccgcagc ggcctggtca acgttggtgt ttaccagacg
    18301 gaattccagc gttgccgtcg cacccagaat ctctttcgca cgcgcagtgt cctgaatacc
    18361 tggcagttca acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc
    18421 aagttggttt acacggttac gcaggatatt gatgttctgc tgcaccgcat attcacgcgc
    18481 ttcactcaga cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctactaat
    18541 caccaggtcc ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc
    18601 gcggaaagta atgctcaggc cgtagttgtt ttctttacga acggtggtat acgggatgcc
    18661 cttttcacgc agatcgctgc gcaggctatc gatattttgt tcctggagtt tgccaagcgc
    18721 ggtgtccata tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt
    18781 catcggctca gcgtgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc
    18841 cacgacgtat ttgtcaccca taacgcccat taatgcttca cgagcgcgca gctgggtgtc
    18901 agtggagtcg aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat
    18961 tttttcttct tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc
    19021 gcgcgcacca gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag
    19081 cagaccgatg acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa
    19141 cacggcaatt cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag
    19201 gacggcagct acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc
    19261 aatgtagccg ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac
    19321 ttcatcacct ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg
    19381 tggacgcagg atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga
    19441 catcgggcta ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct
    19501 cattaaattt ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct
    19561 gacgctggta aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta
    19621 aacccgccat caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta
    19681 atatttcgtt gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt
    19741 agcaatcaca ctcaggatcg agtgggccag tatcgctctt atacttcgca ttgcggattt
    19801 tcaccacgcc atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc
    19861 agtcaaacat atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc
    19921 ccatcaggta acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt
    19981 gcatatctgc tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga
    20041 tatctaccag acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga
    20101 taccaaacag cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc
    20161 aacgcagaga catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac
    20221 actcatcaaa gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt
    20281 caggatcgag gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg
    20341 tgattttacg aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg
    20401 gccccttcca ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg
    20461 ggcgcagcca caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt
    20521 cttccggcgt catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt
    20581 ccactacgcc acaatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca
    20641 gttcaaattt cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg
    20701 ccgcggaatt actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc
    20761 gcatcaccgt aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg
    20821 gtgtgttgat aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg
    20881 aagtttgtca ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg
    20941 gtatcgtcaa agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc
    21001 gaacgtactg aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt
    21061 accgcgtcta ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca
    21121 atggtgtcga cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc
    21181 atctccacgc ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg
    21241 gtcggggctg caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca
    21301 gcgtcttcgt ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga
    21361 atatccagca ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg
    21421 gtcattgttg cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc
    21481 gctttcgagg cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc
    21541 acttcaatct tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta
    21601 ttattaaaaa ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg
    21661 tgcgtcagcg cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc
    21721 atgggatagt gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg
    21781 acactgactc agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa
    21841 cctttacccc cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc
    21901 ccatctcgcg gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc
    21961 tgaagaaagt tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt
    22021 attaaccgac aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg
    22081 ccgcgttgcg cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc
    22141 gcagctgtcg ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat
    22201 gacgattttg ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga
    22261 gcagtggctg gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc
    22321 aagccgccgc ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta
    22381 tgacgccctc gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca
    22441 caaggcgtta taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga
    22501 atcccgtcag tcagttgccc atgcccttct ttgcattgcc attgcacggc attgagcaac
    22561 ggtgacgcgg gtagcaccac ttcacaagct tcgccacggt tgattgccac cagtacacgc
    22621 tgctgattca gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag
    22681 ccgccatgac gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg
    22741 aataacgccg tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg
    22801 tttttgccat ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg
    22861 aacagccaga ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat
    22921 cgcgcagtat cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc
    22981 cctgcgcggt aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg
    23041 taagagatat cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc
    23101 atggcggcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg
    23161 acaatatacg cttccggctg ggtttctttc gccgcttcgg tgatcccagc aacgtgctgc
    23221 atattattgc gcgccccacc cgcctccccc agcatatgca ccacatccag ccgccagccg
    23281 tccatactcc acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt
    23341 tcattcacca gactttccga ctgataatcc agcttcggca agctggcata gccaagccag
    23401 tcgagcgccg tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg
    23461 tgacaagctc cccccgtgcc acgattgtgc ctgtcaaacc aggcatggga atcgccactg
    23521 tggttaaaca cgccatccag caccagccgc attcccagct gctgcgtatt gtggcgtaag
    23581 cgcagcaacg ccccatcacc gtcaaactgc ggatcgacat ggcgataatc ctcggtatcg
    23641 tatttatgta cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc
    23701 tttttcagat acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat
    23761 ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct
    23821 gcatgatggt aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga
    23881 tcagggaaga tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca
    23941 tcgacggcaa actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac
    24001 caacgctggc gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg
    24061 gagagatcaa tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgctgctta
    24121 tgcatcgata ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt
    24181 gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat
    24241 gggggcaccg gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat
    24301 caccttgcca tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg
    24361 acgggggcgg ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag
    24421 aacgcaagta ttattggttt tcagccagct gacgatcgtg gcggcgttta aacatccagc
    24481 caatcaacag cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt
    24541 gataaccaat caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta
    24601 ccccacccgg cactttaaat ttcagcgcct taacttcttc tggcggcaaa cggcgacgga
    24661 aggcaatttg cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg
    24721 aagcgatcac caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca
    24781 gcgcggtagt catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga
    24841 aaattttcgg cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaata
    24901 cgtcactgtt aattgccgac agcgaagcag tcagcacaac aaagttaaga atgctggcgg
    24961 caaaggtaat gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct
    25021 gattccacgg gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac
    25081 gcatcggcac ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg
    25141 cggtaatccc gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca
    25201 ccatcccaag ccagccgtta ctgaagaagc cgccgttgct ccacaggtta tgaataccgg
    25261 tcggttgccc gccgttgcca atcccccaga tgatgatgcc gaaaccggcg acaatcatga
    25321 tgatgatggt ggcgacttta aagaacgaga accagaattc cagctcaccg aagaccttca
    25381 cgctcatcag gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg
    25441 gcaccgtcgg gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga
    25501 caataaggat ttcaaagcaa taggtccagc cggtaatgta acctgccagc gggccgaggt
    25561 tttcctgcgc ataacgcgag aaagagctgg ctgccgggtt atgtaccgac atttccccca
    25621 gcgcacgcat aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg
    25681 gaccggccat tttgatggcg tctgccgaac cgtaaaacag cccggtgcca attgctgaac
    25741 ccagtgccat aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt
    25801 ccatcaattc ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt
    25861 aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat
    25921 cccagataat ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt
    25981 cggccagcgg taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga
    26041 tcccgtcgag aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg
    26101 aattatgcca ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa
    26161 tagcggtcag caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca
    26221 ccatcgagaa gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg
    26281 caaagaattc tgcacaagca caggtcaggc caactgccgt taccaggcag gcgatgaaga
    26341 ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca
    26401 gaatagcagc gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga
    26461 acagcgccag gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat
    26521 aacgggtcag cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga
    26581 taccaaacac cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag
    26641 aaaacgcagc gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa
    26701 tcgcggcaac agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca
    26761 cggtatccag cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca
    26821 ggctgtagat aaacagcggc agcgcggaat cacccgtcag cggcgcaatc cccacttcaa
    26881 aggaaacggt agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg
    26941 ttgccagcag tacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc
    27001 caacttttgc cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc
    27061 cgaatgccgc agtccagacg tgttcgcctg cctgtaagcc gaccattgga gggaaaataa
    27121 tgttacctgc gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg
    27181 atcttaattg atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt
    27241 tttgtgccaa tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac
    27301 tcccaatcag gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg
    27361 acagcacctg aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa
    27421 accagaggta tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa
    27481 agacatggct aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat
    27541 tttgactgca aagaaaataa gccagcatcg gctggcttat ggcaataaaa gatgacaaag
    27601 gcggattaat cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt
    27661 ccttttccta ctgtactctc aatattcagg cgactttcgt gatgattcac cgcatgtttc
    27721 acgatcgcta accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga
    27781 taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca
    27841 acgctaaatt cggcaccgtg cggcactcgc agccagcgta cggtgatatg cgtgccttcc
    27901 ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg
    27961 ttgccagaca ccttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc
    28021 agagtctgag cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg
    28081 agcaattgcg tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct
    28141 tccatccgct gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc
    28201 agcggctgct catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac
    28261 tcatggctca cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg
    28321 acatcacgcg ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga
    28381 tgccgcccgg tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat
    28441 tgcgtaaact ccgggtaacg cagcaggtta aggatgttct gcccgttatc ttccggccag
    28501 cgcaaaccaa gaatttgttg cgccagaccg ttacaccaga aaataccgcc ctcttccgtg
    28561 gtcagcacca ctgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg
    28621 cccagttcac ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc
    28681 ggttcccagc taccacgccc cggtggcggg gtcatactac gatccaccca cagccaccat
    28741 gaaaggcgca ataaattcca gaaatgccag ataagcagtc ccgttaccga tgccagcaaa
    28801 aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc
    28861 agctccagca ccagcctttt ccacgacagc cgttccagca cgtaagatac tccagttaag
    28921 aaatcataag ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga
    28981 tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta
    29041 cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag
    29101 acgtggttta acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc
    29161 agcagtttaa attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga
    29221 gatgtcgggt cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa
    29281 atacggcgca ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg
    29341 atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg
    29401 gtcaacatca ccactggaat atcccgggtc atcgactcgc gcttgaggtg tttgatgaac
    29461 tggataccgg agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca
    29521 ttcagttgat tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt
    29581 tcgagcacga agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt
    29641 ctcgccatga tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat
    29701 gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt
    29761 tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt
    29821 ttcgccaggg aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact
    29881 tctacagtaa aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag
    29941 cacaaaccca tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc
    30001 cgcccagtta cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct
    30061 gtcatctggt ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg
    30121 atatcatggc gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct
    30181 tgcctcgtcg cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccac
    30241 gtgacattat taccagccag gcggggctta acggtattga aaaacagcag catttactgg
    30301 cagcgattac cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc
    30361 agcctctgcc catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg
    30421 ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag
    30481 ccgactacat cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg
    30541 ttcgctattg cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg
    30601 tccatctggt gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg
    30661 taacgcaacc catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac
    30721 agtggcgcga tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg
    30781 agtatctgca tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag
    30841 tattgctggt acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg
    30901 aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac
    30961 tggatgaatc gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg
    31021 ccggagaaca cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa
    31081 aggcgaatgg aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat
    31141 taccggccca acaggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta
    31201 tcatgaaact ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga
    31261 taccgccgaa tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt accgtgcatt
    31321 ctggagccag aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga
    31381 gctggcgcgc tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact
    31441 gacagcgacg ttaaccgggc tggattacgg gcgcttcacc cgttcgatgc tgctttcgca
    31501 ggggcaattt gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga
    31561 gttaaccggc actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc
    31621 ggcccgcaca gagctggaga agctgcaagc gcaggccagc ggcgtcgcgt tgctcacgcc
    31681 ggaacaagtg caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt
    31741 acttaccgcg cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt
    31801 gcagcaagaa gccagccgcc gtcagcaggc cttgcaacag gtgttagccg aagaagaaaa
    31861 agcgcaacct caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca
    31921 ctgggaacgc atcgcagaac acagcgcggc gctggcgcat attcgccagc ggattgaaga
    31981 agtaaatact cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc
    32041 gaagcagtca gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca
    32101 cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca
    32161 aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa
    32221 acttaatgcg cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctaccgccct
    32281 ggcgcaacat gctgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat
    32341 tgttccccaa caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga
    32401 acagacgcaa cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca
    32461 gcaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc
    32521 ccagcgtgca cagttacagg cgggtcagcc ttgtccactt tgtggttcca ccagccatcc
    32581 ggcggtcgag gcgtatcagg cgctggagcc tggcgttaat cagtctcgat tactggcgct
    32641 ggaaaacgaa gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc
    32701 attaacgaag cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca
    32761 agcacttact caacaatggc aagccgtcac ggccagcctc aatatcacct tgcagccaca
    32821 ggacgatatt caaccgtggc ttgatgcaca agatgagcac gaacgccagc tgcggttact
    32881 cagccaacga catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta
    32941 tcaacagcaa attgaacaac gccagcagca gctcttaacg gcattggcgg gttatgcact
    33001 gacattgcca caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca
    33061 gagctggcag caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac
    33121 gccgattctg gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc
    33181 gctggataac tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac
    33241 attacagcaa caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga
    33301 caccgcgcta caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga
    33361 tgaacaaaca ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca
    33421 ggcgcaaacg ctggtcactc agacagcaga aacgctggca cagcatcaac aacaccgacc
    33481 tgacgggttg gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca
    33541 ccaaaagttg cgtgaaaaca cgacgagtca aggcgagatt cgccagcagc tgaagcagga
    33601 tgcagataac cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa tgacgcagca
    33661 ggttgaggac tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg
    33721 caagtttgcc caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac
    33781 ccggctgcac gggcgctatc tgttacagcg caaagccagc gagacgcagg aagtcgaggt
    33841 tgttgatacc tggcaggcag atgcggtacg cgatacccgt accctttccg gcggcgaaag
    33901 tttcctcgtt agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg
    33961 tattgactcg ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac
    34021 cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca
    34081 cgtagaagcg atgaaagagc gtattccggt gcagatcaaa gtgaaaaaga tcaacggcct
    34141 aggctacagc aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa
    34201 tacagagggg cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta
    34261 ttggctacgg tgattggtag ccgttctggt agttgtgatg gtggtatgaa aaaagtcatt
    34321 ttatctttgg ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg
    34381 ctcacggagc tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg
    34441 tattatgcgc tgggagtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac
    34501 tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc
    34561 acgctctctt cgtcttacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat
    34621 ggcgcatttt ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc
    34681 accgccgccg tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg
    34741 ctgggaacgt atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt
    34801 tttaatattg cggtgatggc atcggtctat ttttgggtgc cggatattcg cgacgaggcg
    34861 aaaggaaagc tgcgcgaaca atttcacttt ttacgcagcc cggccccgtg gttaattttc
    34921 gccgccacga tgtttggcaa cgcaggtgtg tttgcctggt tcagctacgt aaagccatac
    34981 atgatgttta tttccggttt ttcggaaacg gcgatgacct ttattatgat gttagttggg
    35041 ctagggatgg tgttgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg
    35101 cgcattgcag cagtgactga ctttataatt gtactggcac tgctgatgct ctttttctgc
    35161 tgcggcatga aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc
    35221 ctttcagcac ctctgcaaat attgttacta caaaacgcca aaggcggaga gttattaggt
    35281 gccgcaggtg ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt
    35341 atgatgctga cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg
    35401 tttgctgcga tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat
    35461 agtccggtgc tggcgaaacc actggggtag attactgtca cggtgcgcgt tgcttattgc
    35521 cggatgcggc gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga
    35581 taagacgcgg caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt
    35641 gtggccataa ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca
    35701 ccggcgtttc acattcgccg ccgaagacaa attgtttaat caactgcgga accgtttgat
    35761 ataaacggtc tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat
    35821 tcacgacatg tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg
    35881 ctaccggatc gctttcttca accaggctga taatttcact gcctttcagc gcatgtccgc
    35941 tcaaacgacg ataatccgtc gcgaatccag tgcccgaaat aaaggtttca atacaacctt
    36001 gtttaccgca ataacaaggg acttcctcgc gataacgcag ttcgtcttcg tccatccacg
    36061 gtagcggatt gtgtccccac tcacctgccg tgccattgcc gccgatatgc gcccgcccat
    36121 tgaatgccac gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc
    36181 ccgctgccgc gccatctacc gcttctgaaa ccgccagaca gttggcgtca tttgccagcc
    36241 gcacttcccg ctgcaaccgc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg
    36301 ttgaattggc attcttcacc acaccggtgt agggcgaaat cgaacctgga atgcccatac
    36361 ctaccgttcc gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt
    36421 caatagtttg ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc
    36481 ctgcatcgcc cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca
    36541 cggtactctc cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa
    36601 tgcaaggccg ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa
    36661 ttatcatgct gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc
    36721 gtgcagaaga gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg
    36781 acatggcgaa gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg
    36841 ttgccaatgg tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg
    36901 tgattaaaca ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc
    36961 tgaagaaaac cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg
    37021 ctttcagccg ttttagccag acaatgatgt ggatcgacac ggttaacggt ttgattatgg
    37081 tggactgcgc cagtgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg
    37141 ggtcgttacc ggttgtacca ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat
    37201 gggttcgctc cggtagtgcg gcacagggct tccagctgct tgatgaagcc gagctgaaat
    37261 cgttgctgga agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga
    37321 tcaccaatca cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc
    37381 gcattcagtt tgtgatgtgc gacgatggtt cgctcaagcg tctgaagttc tgcgacgagc
    37441 tgcgcgatca aaacgaagat atcgaccgtg aagatttcgc ccagcgtttt gatgccgatt
    37501 tcatcctgat gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg
    37561 gcgaagcaca acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc
    37621 ttatccggcg tacgaagcca caccaggcat ataattattc gctacggcgg gcaataattt
    37681 ttagcgcagc aatattatgc gttttatgct gtaacttgct ccatggacgt tgtgtcattg
    37741 tttttcctca agccagtgac gcgcttcact gcgaattttc cgcattgccc cggttttcac
    37801 taaatcccgt aacagcgaaa gatcgggttg ttcgacaaat aagagtaatg atggatcctc
    37861 ttttaaccac gtcgttttca catcggcagc gagctgtgga ttattcatag ctagcgaccg
    37921 atgccgagac tcggtcaatg tcgttaacaa cgacgccggg gtatgtgcgt tgcgtaatgc
    37981 cgtttggcgc aaatgatcat tgcgactttg cagcaatgca cgccaccatt catcatctga
    38041 tttatctttt atcgtagcca gttccgcaac aatatctgga gatcttcact gatatttata
    38101 tcaaccttga tgaattatga atataccgga aattgaaaaa atcgcagcaa aggaaatatt
    38161 attatgggtt aaagagagga tatttcagaa aaatgccctc ccgttcaagg ggaaggcgag
    38221 gaattacaga tagcggcaca gataagaggt gggttcggca acttgcagat gaaactcact
    38281 gtgaccggga acattaaaca ccgaaccggc ttcatacacc tgccagtcgg tcgcgtcagg
    38341 cagtaacaca ttcagcgcgc cactgattac cgtcatctct tccggctcag cggtgctgaa
    38401 ggtgtattcg ccttcaacca taacacccac gctggcgcga ccagtgctgc tgctggaaaa
    38461 gccgattgat ttcactttgc cggaaaagta ctcattactt tgaagcataa actggccctg
    38521 tctggtagtt gaacatctac ttcactatag gggccagagg cgctggctgt cacgcaaaat
    38581 tacaccagta attccgcagc cagccgtgca atcaatacgt tagacagcaa gacaggaaca
    38641 tcgagctgtt tttgcagtaa atcgcgatga cgttggtgaa atcctaaaca atccagcatg
    38701 atgacatcag ccccttttgc cagtaattct ttcccggcat caatgatttt ttgttctgaa
    38761 tcatgaatgg ggttgcccaa tgaaaatacc ggcgatttct gcaaaatttg ccatttttgc
    38821 gcctgaacgg tcagcatctc ctcaaccgga acgataaccc ccacctgatg atcttcaaca
    38881 atagaggaaa ccagtggagg caatattcgc gacggctcaa gaaagatcgt attacgcgca
    38941 gtcatactac taatgtttgc tgtactcatt aataaaatga cgtcataacc ctgattatcg
    39001 agcacttcaa ccacaccttg caggtctcgc tccactttac gacgcgaaac atgggccagc
    39061 tggttgtcat ttaataatgt gagaatggtg tcttcgcctg cttctggcgc gtactccgcc
    39121 atcacttctt cacgacttaa cttccccagc aagctatgat gggaaatatt atcttcgtca
    39181 atgtattccg tcaggagcgg caaaacctcc tgcataggta caatgccgat ggtcaggatc
    39241 gccaacgacg cactcattgt tcatccacct tacttttctt tcgtcgttaa ttaccgggca
    39301 agtgtgaagc accactgctg acacttactt gtgctacaaa tgacaaaaag cgtagcagca
    39361 gacgtgcacg gcataatgta aagattccaa atgattccag taatggattg ttattgttta
    39421 atattctaat tattagaaaa acatgaatta tgaaaaatgt gacgcagatc ccttgtagtg
    39481 gcactcagaa tcccttccga gcagtctgat gcggtcactc tttgtcaggg tcctcatagc
    39541 gttttttcgc atccatcgct tcctgagccg tcggatgttc actgatcaac gagtctggtt
    39601 taggatgatc ggctctgagt tggtaccagg ttaccgtctg tcctggcttt cctttttcaa
    39661 tcgtcactat atatgcttca cgaggataag gcggtttcgt tggcatcgtt cttccttatt
    39721 tcacgggatg aacgttaagt ataggcgctc taaaatcaac aattgatcgt ctgtgccagg
    39781 gcgctacgaa tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat gataatatgc
    39841 gcaacttcgc gatatagcgc atcgcgttct tccagcactt cctgaacttc ttcgctcagc
    39901 ggttttcccg ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag tcggttaacc
    39961 aggactgata ctggcgcaca caaataaacc acgatcccgt tattttgcat gaagtgacga
    40021 ttaaattccg tcagaataat gccgccgcct gtagcgataa cggtggatgg cgcagttacc
    40081 gcttccagcg ccgccgtttc tctggcgcga aatcccgccc actcttccct ttcgacgatc
    40141 tccgcgaccg tcatattgag ctgtgattgc aaccactgat cggtatcgac aaaccgacgg
    40201 ttaagcgaat cggcaagggc cattccgacc gttgttttac cacagccccg aggcccgatc
    40261 agaaaaagag gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg gcgaaagccg
    40321 gtgtcatgag aatagcgatc atgccatcaa actagtacaa tttcgattgt aaagaaaaaa
    40381 ttccacttaa agtgaaaatc tcaatacatc ccttactata ccaataaata ttcaagaatg
    40441 aagtgtaaat aataaattac atttagccac gactacgttg cacttccagc caccacttct
    40501 ccagctccgc ggcaaaggcc tggcggtcac gttgtgaaag gctatctggt ccgccggtct
    40561 ggatcccact ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc tcacgaatgg
    40621 tcgctggcgt gtaacgttcg ccgcgcggat taagcgccgc tgcgcctttc tcgatggctt
    40681 cagcagccaa aggtatatct gcggtgatca ccaaatcgcc cgcttcacac tgccggacaa
    40741 tttcgttatc ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata aatcgcgatg
    40801 gcggcacgcg taaactctgg tttgctacca gtaccagcgg catctgcata cgttccgccg
    40861 cgcgatacaa aatctcttta attacattgg gacacgcgtc ggcatccacc caaattgtca
    40921 taaagtcatc ctttgttggg taatcctcta ttgtgtcgcg cttttgcctt ccggcatagt
    40981 tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag gagtgaggca
    41041 atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat tctcggcggt
    41101 ctgattgcca gcggtcaggt gcttccaggg caaatctggg tatacacccc ctccccggat
    41161 aaagtcgccg ccctgcatga ccagttcggc atcaacgccg cagaatcggc gcaagaagtg
    41221 gcgcaaatcg ccgacatcat ttttgctgcc gttaaacctg gcatcatgat taaagtgctt
    41281 agcgaaatca cctccagcct gaataaagac tctctggtcg tttctattgc tgcaggtgtc
    41341 acgctcgacc agcttgcccg cgcgctgggc catgaccgga aaattatccg cgccatgccg
    41401 aacactcccg cgctggttaa tgccgggatg acctccgtaa cgccaaacgc gctggtaacc
    41461 ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc ggaagtaatt
    41521 gctgagccga tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc ctacgtattt
    41581 atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg cgcccaggcg
    41641 tataaatttg ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga aacgggagaa
    41701 catccggggg cactgaaaga tatggtctgc tcaccgggag gcaccaccat tgaagcggta
    41761 cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac gaagtgtatg
    41821 gaaaaatcag aaaaactcag caaatcctga tgactttcgc cggacgtcag gccgccactt
    41881 cggtgcggtt acgtccggct ttctttgctt tgtaaagcgc caaatctgcc gatttcaacc
    41941 actcacgata gtgactcatt tgtgggttca gcggcgcaac ccccacacta atccgtaaag
    42001 ttacctgtgg cgtattcggc aagcgtaacg tgtttagccc ttcacgcacc cgtaacatgg
    42061 cggtaatggc gctctcagct ggcgtaccgg acatgattac tgcaaactca tcgccgccaa
    42121 accgaccaat cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta agcgccacaa
    42181 tcgcttcatc gcccacatca tgaccccagg tatcgttgat gctcttgaaa tggtcgatat
    42241 cgataatcag taacgttgca tcgcgattat gccgccgaca gttatcaaat tcattgcgta
    42301 acatagtttc ccaatgacgt cggttataca cgcccgtcat gccgtcgcgg gtactcatga
    42361 cctgcaacct gcgtttatgt tccgccagtt tggttgccgt ctggtagctg acccagccaa
    42421 acagcagagg ataaacgaca ataatgggaa gggagagcca ccattccagc ggcgcactat
    42481 tgaacgacac ggtaatgccc gtaagctcga gggtgacaag gcaggaaacc accatcaaca
    42541 ccagacccgc gacaaacaga cgggggccgc ctgcccccat caaattcaga cacataatca
    42601 tcaacatagc ggtggaaggc agcacgttta cgcccattac gcctacccac attcccgcta
    42661 atactgcatc ggtttttaag ttgtaaattt cccggctaag cggatcgacg gccctgctcg
    42721 ctatctgcca ggctaaatgc ggccagacga acgcccagcc gaccaacacc agccaccacc
    42781 agcccggcgg cgggtgcgaa accagcgttg aagcaatcgg taagaacatg ccagccaggc
    42841 caaccgcacg gggtagtctg acgcgacggg cgaagcgcag cccggaccgc tgatgttcgt
    42901 tttgaggagt taaaggaggt tcctccccat gcgccgccgc ttttttgaaa aagttttcat
    42961 cattcattat ttttgggaac attcagaaac aattttccca aattatagag acggggctaa
    43021 aggcagaagt atgaaatttc gggtgagcca ctttcgtagc tcacccgtta gcactatcag
    43081 gtatcaggcg gctttcttga ggcaggcact cataaactta ttacgatcat cacctttcag
    43141 agattgttgc gttgcttgat tattacattc gcgcatcttt tgctgctgtg gcgtcaaact
    43201 tttttcacca ggcgcagact tgctgttctt caggcaatca ctcatgtagg tcttacgagc
    43261 atcccctttc aacgcctgcg ccgtcgcctg ctgattacag gatgtcatac gctgttgttg
    43321 tggggttaag gttctctcgg cagcgccgac ggtggttaaa aaaaccagac caaaaagcaa
    43381 ggtaaccagt aatgttattt tcatagcacc atccctcttc atgttttaac catgagcgta
    43441 tgcgcccgtg atctgccatt aagtctggtt gctaacagca aaaaaaccac ccggcagcga
    43501 aaattcactg ccgggcgcgg ttttatttca gccccagggc ggctttcatg gtgtagaaga
    43561 gatcggtctg gtcggtcagt ccaacaacat tggcggcatg cgggccatac gccgcaatac
    43621 gcaactgact gccggtatgt tcttgtgaat cctcttcgga gttcccgtag ctcatcacca
    43681 tcactgcgcc atctttggta tttagcgcct gggtgaggcc cggagctttg gtatccggcg
    43741 cgacaatctg gctggcgtgg gcgtgatcag cggtgactat gaccagcgtg ttgccatcct
    43801 ttttagcgaa ttccagcgcc cgttgtacgg cttcatcgag atcgaccgtc tcgccaattt
    43861 gcccacaagg attcgcagcg tgatcctgtt tatcgattga cgcaccttca acttgcagga
    43921 aaaatccttt ctcattttta ctcaacaatt caatggcttt gtcggtcatc tgcgccaggg
    43981 ttggtacact gtcattacgt tgcggattag gcgtacaggt gactgcgggc ttatcgatat
    44041 tgccgtggta cgttgctttc ggtcctagcc agcgcactgg catattgccg tcagcaaaca
    44101 gtccaagcag aggtttttgc tgattcgctt ccgtcaccga attcagtgag gcagcatcgc
    44161 tgaccaactg ataaccacgc gcctgtgcct gttcacgcag cgtttttccc tgccattcac
    44221 cggcggttgc cgtttcagca aaggtttttg cgccaccgcc aagcgtaacg tcggcacgag
    44281 cgttaagcag ctgttcggta atcgatcctt ttccgccttt ttccagagcg ttacccggac
    44341 atttttcact ggtcgcgctc ggaccgtagc atttgcgcga ggtcacatgc gccaccagcg
    44401 cagcgggcgt ggcatcctgc aactctgcgg tagaaacgtt accggtcgcc agacctgcgg
    44461 cttttgccat ttccagaatc gttgggtgat ctttttcgtg aatatcgacg cccagcgcgc
    44521 cgttataggt tttgacaccg gttgaccagg cggttgctga tgcagccgaa tcggtgacgt
    44581 agtccggttt gccggttttt ttattcagcg catagtgagt gtattgcccg gtaagcggta
    44641 aggcatcgat acctttaaaa aagccgcccg caccttcggc ataattacgt gcggctgtaa
    44701 tttccgaatc ccccatccca tcgccaatca gcaaaataat attttttgca ggtttatcgc
    44761 taagagaatc acgcagagcg gcagtctgat cacccgttaa acggcgagca ccgccggatg
    44821 cagtaatatc gccctgagca gcccggtttt ccagaacagg catttctggt gtccgggctt
    44881 ttgtcacagg ggtaaacagt aacgataaga gtgccagtgc aatagtgctt tgtttcactt
    44941 tattttctcc atgtacaaat acattaaaaa ataaaaacaa agcgactata agtctcggcc
    45001 gtgacaactt tatgacagct gttgaaaaga ttaacttttt actgacgagg atgcttcaat
    45061 aacttcttta cgtaatcgcg cagcagctcc gtatcgtcgt caggaatgct ggcatcgggc
    45121 tttacctcgt acagcgcccc ctctacctga tcaatcaagc gctgttggtc attttgcgcc
    45181 atattgcgaa gcattgcagt gacgataatc tccaaagctt ctacctgcgc acacagttct
    45241 ttcgactctt cttctttttg ggcaagctta aataacaact cagcaatgag atttttcatg
    45301 tctgtatttc cttatccaaa gtatggagaa gttaacatta tgtttcacaa cttcatagtg
    45361 tctggtagta ttatttttat gacaaaggaa atactttgcg caaatatttt ttgtttgatt
    45421 accagcgaaa cgtttctctt atttaaataa ttac
//
LOCUS       DAZGLJ010000013        45274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000013 DAZGLJ010000000
VERSION     DAZGLJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.013"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            229..813
                     /gene="yadN"
                     /locus_tag="ACHK49_000659"
     CDS             229..813
                     /gene="yadN"
                     /locus_tag="ACHK49_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9261350.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            918..1658
                     /gene="yadV"
                     /locus_tag="ACHK49_000660"
     CDS             918..1658
                     /gene="yadV"
                     /locus_tag="ACHK49_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO9261351.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1693..4293
                     /gene="htrE"
                     /locus_tag="ACHK49_000661"
     CDS             1693..4293
                     /gene="htrE"
                     /locus_tag="ACHK49_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFO9261352.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            4310..4879
                     /gene="yadM"
                     /locus_tag="ACHK49_000662"
     CDS             4310..4879
                     /gene="yadM"
                     /locus_tag="ACHK49_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9261353.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLSGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            4894..5496
                     /gene="yadL"
                     /locus_tag="ACHK49_000663"
     CDS             4894..5496
                     /gene="yadL"
                     /locus_tag="ACHK49_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO9261354.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            5523..6119
                     /locus_tag="ACHK49_000664"
     CDS             5523..6119
                     /locus_tag="ACHK49_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO9261355.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            6103..7446
                     /locus_tag="ACHK49_000665"
                     /pseudo
     CDS             6103..7446
                     /locus_tag="ACHK49_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            7559..8353
                     /gene="panB"
                     /locus_tag="ACHK49_000666"
     CDS             7559..8353
                     /gene="panB"
                     /locus_tag="ACHK49_000666"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO9261356.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITDALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8365..9216
                     /gene="panC"
                     /locus_tag="ACHK49_000667"
     CDS             8365..9216
                     /gene="panC"
                     /locus_tag="ACHK49_000667"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO9261357.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIIRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9290..10192)
                     /gene="rpnC"
                     /locus_tag="ACHK49_000668"
     CDS             complement(9290..10192)
                     /gene="rpnC"
                     /locus_tag="ACHK49_000668"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO9261358.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            10466..10846
                     /gene="panD"
                     /locus_tag="ACHK49_000669"
     CDS             10466..10846
                     /gene="panD"
                     /locus_tag="ACHK49_000669"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO9261359.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10850..12079)
                     /gene="yadE"
                     /locus_tag="ACHK49_000670"
     CDS             complement(10850..12079)
                     /gene="yadE"
                     /locus_tag="ACHK49_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO9261360.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12143..12583)
                     /gene="yadI"
                     /locus_tag="ACHK49_000671"
     CDS             complement(12143..12583)
                     /gene="yadI"
                     /locus_tag="ACHK49_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO9261361.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDLAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12688..13458)
                     /gene="yadH"
                     /locus_tag="ACHK49_000672"
     CDS             complement(12688..13458)
                     /gene="yadH"
                     /locus_tag="ACHK49_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9261362.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13455..14381)
                     /gene="yadG"
                     /locus_tag="ACHK49_000673"
     CDS             complement(13455..14381)
                     /gene="yadG"
                     /locus_tag="ACHK49_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9261363.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14490..15152
                     /gene="can"
                     /locus_tag="ACHK49_000674"
     CDS             14490..15152
                     /gene="can"
                     /locus_tag="ACHK49_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO9261364.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15193..15729)
                     /gene="hpt"
                     /locus_tag="ACHK49_000675"
     CDS             complement(15193..15729)
                     /gene="hpt"
                     /locus_tag="ACHK49_000675"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO9261365.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15935..18325
                     /gene="gcd"
                     /locus_tag="ACHK49_000676"
     CDS             15935..18325
                     /gene="gcd"
                     /locus_tag="ACHK49_000676"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO9261366.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18372..19922)
                     /gene="cueO"
                     /locus_tag="ACHK49_000677"
     CDS             complement(18372..19922)
                     /gene="cueO"
                     /locus_tag="ACHK49_000677"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO9261367.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIILPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20088..20435
                     /gene="yacC"
                     /locus_tag="ACHK49_000678"
     CDS             20088..20435
                     /gene="yacC"
                     /locus_tag="ACHK49_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO9261368.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20541..21407
                     /gene="speE"
                     /locus_tag="ACHK49_000679"
     CDS             20541..21407
                     /gene="speE"
                     /locus_tag="ACHK49_000679"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO9261369.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            21423..22217
                     /gene="speD"
                     /locus_tag="ACHK49_000680"
     CDS             21423..22217
                     /gene="speD"
                     /locus_tag="ACHK49_000680"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO9261370.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22255..22617)
                     /gene="yacL"
                     /locus_tag="ACHK49_000681"
     CDS             complement(22255..22617)
                     /gene="yacL"
                     /locus_tag="ACHK49_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO9261371.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22792..25389)
                     /gene="acnB"
                     /locus_tag="ACHK49_000682"
     CDS             complement(22792..25389)
                     /gene="acnB"
                     /locus_tag="ACHK49_000682"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO9261372.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHSL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25744..27501
                     /gene="yacH"
                     /locus_tag="ACHK49_000683"
     CDS             25744..27501
                     /gene="yacH"
                     /locus_tag="ACHK49_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO9261373.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(27572..28996)
                     /gene="lpdA"
                     /locus_tag="ACHK49_000684"
     CDS             complement(27572..28996)
                     /gene="lpdA"
                     /locus_tag="ACHK49_000684"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO9261374.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29321..31213)
                     /gene="aceF"
                     /locus_tag="ACHK49_000685"
     CDS             complement(29321..31213)
                     /gene="aceF"
                     /locus_tag="ACHK49_000685"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO9261375.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31228..33891)
                     /gene="aceE"
                     /locus_tag="ACHK49_000686"
     CDS             complement(31228..33891)
                     /gene="aceE"
                     /locus_tag="ACHK49_000686"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO9261376.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34052..34816)
                     /gene="pdhR"
                     /locus_tag="ACHK49_000687"
     CDS             complement(34052..34816)
                     /gene="pdhR"
                     /locus_tag="ACHK49_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO9261377.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            35357..36730
                     /gene="aroP"
                     /locus_tag="ACHK49_000688"
     CDS             35357..36730
                     /gene="aroP"
                     /locus_tag="ACHK49_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO9261378.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAMIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(36773..37627)
                     /gene="ampE"
                     /locus_tag="ACHK49_000689"
     CDS             complement(36773..37627)
                     /gene="ampE"
                     /locus_tag="ACHK49_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO9261379.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPRAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(37624..38175)
                     /gene="ampD"
                     /locus_tag="ACHK49_000690"
     CDS             complement(37624..38175)
                     /gene="ampD"
                     /locus_tag="ACHK49_000690"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO9261380.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            38263..39156
                     /gene="nadC"
                     /locus_tag="ACHK49_000691"
     CDS             38263..39156
                     /gene="nadC"
                     /locus_tag="ACHK49_000691"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO9261381.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            39359..39799
                     /gene="ppdD"
                     /locus_tag="ACHK49_000692"
     CDS             39359..39799
                     /gene="ppdD"
                     /locus_tag="ACHK49_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO9261382.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            39809..41194
                     /gene="gspE"
                     /locus_tag="ACHK49_000693"
     CDS             39809..41194
                     /gene="gspE"
                     /locus_tag="ACHK49_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO9261383.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            41184..42386
                     /gene="hofC"
                     /locus_tag="ACHK49_000694"
     CDS             41184..42386
                     /gene="hofC"
                     /locus_tag="ACHK49_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO9261384.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(42421..43464)
                     /gene="guaC"
                     /locus_tag="ACHK49_000695"
     CDS             complement(42421..43464)
                     /gene="guaC"
                     /locus_tag="ACHK49_000695"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO9261385.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(43620..43664)
                     /locus_tag="ACHK49_000696"
     CDS             complement(43620..43664)
                     /locus_tag="ACHK49_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9261386.1"
                     /translation="MIIKTLKMSARKRN"
     gene            43689..44309
                     /gene="coaE"
                     /locus_tag="ACHK49_000697"
     CDS             43689..44309
                     /gene="coaE"
                     /locus_tag="ACHK49_000697"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO9261387.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            44309..45052
                     /gene="zapD"
                     /locus_tag="ACHK49_000698"
     CDS             44309..45052
                     /gene="zapD"
                     /locus_tag="ACHK49_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO9261388.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            45062..45259
                     /gene="yacG"
                     /locus_tag="ACHK49_000699"
     CDS             45062..45259
                     /gene="yacG"
                     /locus_tag="ACHK49_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO9261389.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
BASE COUNT        11335 a        11740 c        11018 g        11181 t
ORIGIN      
        1 ccaattgcac ttgtgaattt atttttgctt aattttgaat taagaaaatt taatttgttc
       61 aaaagccctt aaagtgcaca ataggcgtcc taatgaacaa tggttgtaaa ttttggtaac
      121 aaatatcatt gctcattaat atctagcatc tgcttataac atttgtgtca tgaatagtca
      181 ttcttcaaac cagtagtctg actaatggaa ttttattcaa ggatttttat gtctaaaaaa
      241 ttaggttttg ccctgtctgg tcttatgctg gcaatggttg caggtactgc ttctgctgat
      301 atggatggtg gtcagttaaa tatcagtggt ctggttgttg ataacacctg tgaaacccgc
      361 gttgacggcg gtaataaaga tggtctgatc ctgctgcaaa ctgcaactgt tggtgaaatc
      421 gatgctggcg tgctgaatga caccgttggc gctaaagcca aacctttcag catcaccgtt
      481 gactgcagca aagcaaatcc gaatccaggt agtacagcta aaatgacctt cggttctgtt
      541 ttctttggta atagcaaagg aacgctgaac aacgacatgt ctattaacaa cccgtctgac
      601 ggcgtgaata tcgctctgca caatattgat ggttccacta tcaaacaggt tcaaattaac
      661 aaccctggcg atgtgtatac taaagccctg gatgcaacga caaaatctgc tgtttatgat
      721 tttaaagcgt cttacgttcg tgccgtcgca gaccaaacag caactgctgg ttatgtaaaa
      781 actaacactg catacaccat tacttatcag taagtattca ccaattaaat tgattgaatg
      841 aatatacagg gaatagtgtt aataatttct atttttatat tattccctgt tttaattaac
      901 tctgtcaggg atggtttatg ttttttaaca ccaaacatac aacagcttta tgcttcgtaa
      961 cctgtatggc ttttagttca tcgtctattg cggacattgt catttcgggt actcgcgtaa
     1021 tatataaaag cgatcaaaaa agtgtcaacg tacgtctgga aaataaaggg aataacccgt
     1081 tgcttgtcca gagttggtta gatactggcg atgacaacgc tgagcctggc agtattacag
     1141 tcccttttac tgccacgccg ccagtatcgc gtattgatgc caaacgtggg caaacaatca
     1201 aattaatgta cacagccagc acctcactgc ctaaagacag agagagcgtg ttctggttta
     1261 acgtactgga agttccacca aaaccagatg cagaaaaggt cgcgaatcaa agtctgctgc
     1321 aactggcatt tcgcacacgt ataaaacttt tctatcgccc ggatggattg aagggaaatc
     1381 cctctgaagc cccgttagcc ctgaagtggt tctggtcagg ttcagaaggt aaggcgtcat
     1441 tgcgagtgac caatccaacc ccttactacg tctcttttag cagtggtgat ttagaagcta
     1501 gcggtaaacg ctatccgatt gatgtgaaaa tgattgcacc atttagtgat gaggtcatga
     1561 aagtcactgg ccttaatggc aaagcaagtt ctgcaaaagt acatttttac gccattaatg
     1621 attttggtgg cgccattgaa ggtaatgcca gtctgtaatc aggcaaggat ataattccgc
     1681 aggaagcata acgtgactat aaaaccaact aaaaattatc ataatcatct gacccgtatc
     1741 gctacgtttt gcgcgctgct gtattgcaat agtgctttct gtgctgaact tgttgaatat
     1801 gaccatacct tcctgatggg gcagaatgcc tcaaatattg atctcagccg gtatagtgaa
     1861 ggtaaccctg ctataccggg tatgtacgac gtcagtgttt atgtcaacga tcaacctatt
     1921 attaaccaaa gcattacatt tattgaaatt gaaggaaaaa aaaacgcaca agcctgtatc
     1981 acacttaaga atttattgca gtttcatata aattctcccg atataaataa cgaaaaagcc
     2041 gttctgcttg ccagggatga aacactcggc aattgcctta atttgacgga aattatccct
     2101 caggcttctg ttcgttatga cgttaacgag caacgtctgg atatagacgt tcctcaggca
     2161 tgggtaatga aaaattacca aaactatgtt gatccatcgt tatgggaaaa tggcattaat
     2221 gcggccatgt tgtcatacaa cctcaacgga tatcatagtg aaactcctgg tcgaagaaat
     2281 gacagcattt atgctgcttt taacggtggg atgaatttag gtgcatggcg actgcgtgcc
     2341 tcaggcaact acaactggat gaccgattct ggcagtaatt atgattttaa gaatcggtat
     2401 atacagcgtg atatcgcctc gctgcgttct caactcattc ttggtgagtc ttatacaacg
     2461 ggcgaaacct ttgattctgt cagtatccgc ggcattcgtt tatacagcga cagccgcatg
     2521 ttgcctccga cattagcgag ttttgcgcct atcattcatg gcgttgctaa taccaacgcc
     2581 aaagtcacta ttacgcaagg tggctataag atttatgaaa cgactgtgcc gccaggcgct
     2641 ttcgtcattg atgatctgag tccgtcaggt tacggcagcg atcttattgt tactgtcgaa
     2701 gaatccgatg gctcaaagcg gacattctcg caaccttttt catcggtcgt tcaaatgtta
     2761 cgccctggcg ttggacgttg ggatattagc ggcggccagg tcttaaaaga tgatattcag
     2821 gatgagccta atttatttca ggccagctac tactacggtc tgaataacta tctaacgggt
     2881 tatactggta ttcagattac cgataataac tataccgctg gtttgttagg tcttggtctg
     2941 aatacttcag tcggtgcatt ttctttcgat gtgactcact ccaatgttcg tataccggat
     3001 gataaaacat atcagggaca aagttatcgt gtttcctgga acaagttatt cgaagaaaca
     3061 agtacttcac tgaatatcgc ggcctatcgc tattcgacac agaattacct tggtcttaat
     3121 gatgcactaa ctctaattga tgaagtgaaa catcccgaac aagatcttga accgaaatcc
     3181 atgcgtaatt actcgcgcat gaaaaatcag gttacggtca gtattaacca accgctaaaa
     3241 tttgagaaaa aagattacgg ttcattttat ctttccggaa gttggtccga ttactgggct
     3301 tccggacaaa atcgtagcaa ttactctatt ggctacagta acagtgcatc ctggggcagc
     3361 tacagtgtca gtgcccagcg ttcatggaat gaagacggcg acactgacga tagcgtttat
     3421 ctcagtttca ccattcctat tgaaaaatta cttggcactg aacaacggac ttcaggtttc
     3481 cagagtattg atactcaaat gagcagtgac tttaagggta ataaccaact caacgtcagt
     3541 agcagtggct atagtgataa cgctcgcgtc agttatagcg taaatactgg ctatacgatg
     3601 aataaagcca gcaaagattt gagttatgtt gggggttatg ccagctatga atcaccgtgg
     3661 ggaaccctgg caggttcagt ttctgcaaat agcgataaca gtcgtcaagt gtctctcagc
     3721 accgacggtg gttttgtatt gcatagcggc ggcctgacct tcagtaatga cagttttagc
     3781 gactccgata cactggcggt agttcaggct ccaggtgctc aaggagcgcg aataaattat
     3841 ggcaacagta ctatcgatcg atggggttat ggtgtcacca gcgctctttc tccttatcat
     3901 gaaaaccgta tcgcgctgga tatcaacgat cttgagaacg atgttgaatt aaaaagtacc
     3961 agtgcagtag ctgtaccgcg tcagggttca gtcgtctttg ctgattttga aaccgtgcaa
     4021 gggcaatcag ccattatgaa catcacacga agtgatggta aaaatattcc atttgctgca
     4081 gatatttatg atgagcaagg caatgtcatt ggtaatgttg gacagggtgg acaagcattt
     4141 gttcgtggta ttgagcagca gggaaatatc agcattaaat ggctcgaaga aagtaaaccc
     4201 gtaagttgtc ttgcgcatta tcaacaaagt tcagaagcag aaaaaatagc gcaatctatt
     4261 attctgaatg gaattcggtg tcagattcag taactacaag ggacgtcaaa tgataaaaat
     4321 aacgccacat aaaatagcga tactgatggg aatattatta tcaccatcag tatttgcaac
     4381 ggatattaat gtagagttta cagccactgt caaagcgaca acctgtaaca tcacacttac
     4441 tggtaataac gtcacgaatg atggcaataa taactacaca ttgagaatcc ctaagatggg
     4501 tctggataag atcgcgaata aaacgacaga atctcaggct gattttaaac tggttgctag
     4561 tgggtgcagc agtggcatca gttggattga taccactctg agcggaaatg catcatcaag
     4621 ctcacctaag cttattatac cgcagtctgg tgattcatct tcgacgacaa gtaatatcgg
     4681 tatgggtttc aaaaaacgga ctactgatga tgccactttc cttaaaccta acagtgcgga
     4741 aaagatacgc tggagcacag acgagatgca gcccgataag ggtcttgaaa tgaccgttgc
     4801 gctacgtgaa acagatgcag ggcaaggcgt accggggaat tttcgtgcac tggcaacgtt
     4861 taatttcatc tatcaataag aaggataatt aagatgacat ttaaaaattt acgttatgga
     4921 ttatccagca gtgtcgtttt ggctgcctca ttgttcagcg taccctctta tgcggcaaca
     4981 gacagcattg ggctgaccgt gattactacc gtagaaatag gtacttgtac cgctacatta
     5041 gtaaatgact ctgatcagga catttctgtt gttgactttg gcgatgtata tatttctgaa
     5101 atcaatgcca agaccaaagt taaaacattc aaactcaaat tcaaagactg tgcgggtatc
     5161 cccaataaaa aagcacaaat aaaattaacc aagcgagcca catgcgaagg tactgctaat
     5221 gacggtgcgg ggttcgcaaa tggatccaca gccgcagata aagcaagtgc cgtcgccgtt
     5281 gaagtctgga gcaccgaaac tccggcaaca gggagcgcaa cacaatttag ttgtgtgaca
     5341 ccagcatctc aagaggtcac tatctccaat gcagccaacg cggttgttta ttatccgatg
     5401 agtgctcgac tggtcgtcga aaaaaataaa accgtaagca atgtcactgc gggtaaattt
     5461 tctgcacctg ccacatttac agtaacctat aactaacaga ctgacagatc caggaactat
     5521 atatgcatcc cactcaacgt aagctgatga agagaataat tctgtttctg tcactacagt
     5581 ttcccattac ctatccagcc atcgctgggc aggatattga ccttgttgcc aatgtaaaaa
     5641 acagcacttg caaaagcgga atcagtaacc agggtaatat tgatcttggc gtcgttgggg
     5701 tgggatattt ttcagataat gttactcctg aaagctatca accaggtgga aaagagttca
     5761 ctgtcacagt atcagactgt gcattacagg gaactggcga tgtgcttaat cagttacata
     5821 ttgattttag agcccttagc ggtgtcatgg ctgcaggttc aaggcaaata tttgccaatg
     5881 aagtcgcaac cggggctaaa aatgtcggtg tcgttctctt ttccattcag gacccgacca
     5941 atatattcaa tgttattagt tcagcaggaa attctcgttc cgtgtatcca gtaatgactt
     6001 ctgccctgca taattcttca tggaaatttt atgccagaat gcaaaaaatc gatcccgcgc
     6061 tggatgtcat atctggccag gtaatgagtc atatattagt tgatgtgtac tacgaataat
     6121 agatcaaaac aaataactgc gactgttaca gcagtagatg gtggagtgat aatgaagaaa
     6181 ttcttcagac attttctttt tttaattctg tgcttgagtt gttataccgc gagtgctggc
     6241 actgatgata acgtaggata tattgttggg aatagttacg gcgttggtcc gtcagatcaa
     6301 aaatggagag aaacggggcc gaatggcgat gctactgtca tattcagata tgcaacaagt
     6361 acaaataatt tagttttcta caaaccgacg cagcttggac ctacaggtgt aaaattacag
     6421 tggagtcagt tagataccgc ttctggtggt ggttttcttt attgcaacag aagtgatagc
     6481 acaagtggta gcgcaatgcg tattgaaaat gcaatggttg actcaggtaa aatgtatggc
     6541 tcccataaat tatttaatac atcagttcct ggtttgtatt acacattatt aatttcaaac
     6601 atgtggtcag cttacggtac cgtaactaac gttagttcac ctgggatata cattggtgac
     6661 tctgcagaac aatatttttc gtggtataat ccaagcgaag acgtgttata ttagagttgc
     6721 aataatgcga atagcacccg taaatactgg gctgtaggtg gtatttatca gacccttaca
     6781 attgaattct atacagatac aaactttgat ccaacagtaa ctcaacagat caaattatcc
     6841 agctcatcaa attatctgta ttcatttaaa gcctatggcg caggccaagg tataaatgag
     6901 catagttatt ttatcaaaat cgatttcgat ctgctcaatg tcaagttgac taatcccact
     6961 tgttttaccg ctatgcttag tggaacttct gtgactggtt ctacggtaaa aatgggtgaa
     7021 tatagtgcag aacaaatcag aaacggtgcc acaccggttc cttttgatat ttcacttcaa
     7081 aactgcgttc gtgtgactaa tattgagaca aaattagttt caacaaaggt tggtactgaa
     7141 aacgggcaac tccttggtaa tactctcaca ggtaatgacg cagcaaaagg agtcggtgta
     7201 ctcatagaag gtttagcaac tagtaaaaat cctctaatga cattgaaacc taatgattca
     7261 aattctgttt ataaagatta cgacccgaga ggcaaagacg atacaacagg aggagtttac
     7321 ccggatcaag atacaggtat aacataccct ctccatttcc aggccacgct acaacaggat
     7381 ggaactatac caatagaagc tggtgagttt aaagccacca gtactttcca ggtaacctac
     7441 ccttaataag tccaccgcac cgccatcatc tggcggtgca gtaattgata aatcattgcc
     7501 cccaccaatg tgactgataa aatgccaggc aacacgacat catttatcag gatacgttat
     7561 gaaaccaacc accattgctt cacttcagaa atgtaagcag gataaaaagc gtttcgcgac
     7621 catcaccgct tacgactaca gcttcgcgaa actatttgct gatgaaggac ttaacgtcat
     7681 gctggtaggc gactcgctgg gcatgacggt tcagggtcac gactccaccc tgccggtgac
     7741 cgttgctgat atcgcctacc acactgccgc cgtacgtcgc ggcgcgccaa actgcctgct
     7801 gctggctgat ctgccgttta tggcgtatgc cacgccggaa caagcctttg aaaacgccgc
     7861 aacggttatg cgtgccggtg ccaatatggt caaaattgag ggcggtgagt ggctggtcga
     7921 aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt ggtcacttag gtttaacccc
     7981 acagtcagtg aatattttcg gtggctacaa agttcagggg cgcggcgatg aagcgggcga
     8041 tcaactgctc agcgatgcat tagccttaga agccgctggg gcacagctgc tggtgctgga
     8101 atgcgtgccg gttgaactgg caaaacgtat taccgatgca ctggcgatcc cggttattgg
     8161 cattggcgca ggcaacgtca ctgacgggca gatcctcgtg atgcacgacg ccttcggcat
     8221 taccggcggt cacattccta aattcgctaa aaatttcctc gccgaaacgg gcgacatccg
     8281 cgcggctgtg cggcagtata tggctgaagt ggagtccggc gtttatccgg gcgaagaaca
     8341 cagtttccat taaggagtca cgttgtgtta attatcgaaa ccctgccgct gctgcgtcag
     8401 caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc tggtgcctac tatgggtaat
     8461 ctgcacgatg gccatatgaa gctggtcgac gaagccaaag cccgcgccga tgtggtcgtc
     8521 gtcagtattt tcgttaaccc aatgcagttt gaccgcccgg aagatctggc tcgttatcca
     8581 cggaccttgc aagaggactg cgaaaagcta aacaaacgta aagtggattt agttttcgcc
     8641 ccttcggtaa aagagatcta cccgaacggt actgaaaccc acacttacgt tgacgttcct
     8701 ggcctttcga ccatgctgga aggtgccagc cgtccgggac attttcgcgg cgtttcgact
     8761 attgtcagca agctgttcaa cctggtccag ccggacatcg cctgcttcgg tgaaaaggac
     8821 tttcagcaac tggcgctaat ccgcaaaatg gttgccgata tgggcttcga tattgaaatt
     8881 gtcggcgtcc cgattatacg cgccaaagat ggccttgcgc tcagttcccg taatggttat
     8941 ctgacggcgg aacaacgcaa aattgcgccc ggtctgtaca aagttttaag ttcaattgcc
     9001 gacaaattgc aggctgggga acgggatctc gatgaaatta ttaccattgc ggggcaagaa
     9061 ctgaatgaaa aaggcttccg cgccgatgat attcagattc gcgatgccga cacattgctg
     9121 gaagtttctg aaaccagcaa acgggcagta attctggtag ccgcctggct tggcgatgct
     9181 cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact taactggcgc tacggctgat
     9241 ggcgccagtt attaatttac cccacgtcat ctgtcataac acaggtaact taaataaggt
     9301 taattacctt atcaatctca gcaagaggta aatttgccat ctctgcaacg tcttcccgag
     9361 acattccttt acttagaaga cgcagggcga attcttgcct ttcctcctgt cttccctgct
     9421 gaatcccctt ctcaatccct ttctcttcaa accactgcgc cagcgtcatc atagactccc
     9481 ctcccgtttc cctgtctctc agcacaccat aaaacaaatc cgcttgttca gtatgaccgc
     9541 gttgcagcat atagttttgc atggcaacta actgacttcc gctagtgtac ccttcgtcaa
     9601 tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg ctggcgaata tgtttttgca
     9661 gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt gtgatggtga
     9721 tatccaccag cgggaaagga ctgttataga cgcgtcgtgc cagctccggc gagtaaaata
     9781 tatcaaacca gcacattgag agcggataag gtgtggcctc gccctgataa aacagtatcg
     9841 gcaccaccag cggcagctta tcgtgatccg cctccagatg ccggtgcatg gcagcaatag
     9901 aataacgcat catgcgaaag gccattttct tatccggctt gctttggtgt tcaatcacaa
     9961 catgcagata accgggattg ccctgcattt gcacggaata gagcacgtcc gtgctgtgtc
    10021 ctttcaggct ctcttcaatg aaactccccg actctaaatg aagcgtgttg aggtcacaaa
    10081 gttctcgtaa ttccactggc aaatgtatct ccagaaagtc gcgagccgtc tccgcgtgca
    10141 ttaaaaattg tttaaacact gcatcatgcg gtgtggtact cggtgcatcc atcttccctg
    10201 ccttccgtgt tgtcttgcgt gaaggcagta tccagtcaag gcgtgccatc gtcagccacc
    10261 tctttagtgt ttaccgagca gcgttcagag aaatttcaat tggttacagc cgttacatgg
    10321 cacgcttcgg cacggtacgc aaactgctga tgtaaatgcc gtcagatgag tagacactaa
    10381 acaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc aggcatcgcc tgcttcgtta
    10441 acgacagggt agaaaggtag aagttatgat tcgcacgatg ctgcagggca aactccaccg
    10501 cgtgaaagtg actcatgcgg acctgcacta tgaaggttct tgcgccattg accaggattt
    10561 tcttgacgca gccggtattc tcgaaaacga agccattgat atctggaatg tcaccaacgg
    10621 caagcgtttc tccacttatg ccatcgcggc agaacgcggt tcgagaatta tttctgttaa
    10681 cggtgcggcg gcccactgcg ccagtgtcgg cgatattgtc atcatcgcca gcttcgttac
    10741 catgccagat gaagaagctc gcacctggcg acccaacgtc gcctattttg aaggcgacaa
    10801 tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt tatccctgcg
    10861 gctggttact caccagccgc gacatcgtct ccagcgaatc cgttcttaag atataaagtc
    10921 gttttagtaa caacggatta tcccccggtt ttactttgcc tttcatggtt gtcaccgcca
    10981 ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc attaaatccg ccaaacggat
    11041 acgaaagata ccagacatgc ggattaaatt gcgccagagc gcggcgtgaa cgtgcaaaat
    11101 caaacagaat attgtgctca ctacggctca gtaatatggg tcggcgatag ccatccaccc
    11161 gatgcaaaaa atgggtatgt gactgaaaat caaaaacatc gcgaatttcg ttaagctcag
    11221 aaacgctcat aaattgcagc gattttgggt tccacttctg cgggtgacgt ttgatgcgcg
    11281 aggtaacaac aaacgccgtc gccttcatgc catattgttt caatacagga tacgcatagc
    11341 ggctcaccga cttgaggcca tcatcaaagg taatcaccac cgctcgcgca gggagattga
    11401 tcttattctt cacgtaacct tccagctgcg ccatgctcag tgtcgcgtat cccctgtcgc
    11461 gcagccaggc catttggtta ttgaaagcgc gtaccgaggt ggtcgtcgaa gtatggcgaa
    11521 aacgggtgtt ttcttcatcg cgcagaatat ggtgataggt tagcaccggc aggccattat
    11581 cgggttgggc atccagcgcg ctgatatagg cgagtcgatc gccaatacgg atctgatacc
    11641 aggtttgatt taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa ttgtccgcca
    11701 gtaccccaaa tggcgcactg cccgcactcg gcgcgttata gaccggcgta tctttccagg
    11761 taattaagtt ctgattactc agcggcttgt tgaggtcacc caaaccgtct tcaacttttt
    11821 gtcgcccctg aactggctcg agatgccctt tatcgataaa ccctttacca aagccaaaat
    11881 taaatgcgta ataacttgcg gcagtgggct ccacggcaat gatttgcccg gcccgaatat
    11941 tccccacggt caccatcttg tcgccaattt gcgcccagat cgcagcattt tcgatggttt
    12001 gcatataacg ggcaggtaac gcggcactga cacttgcggt aaacagcatc agcaggagaa
    12061 taacagcttg tttgtacatg aaaaaataac cgagggtgaa acaacgcttt tattgtagca
    12121 aaagcgctat aagaatcgtg ggttaatact catacaaatt atgtttgagg acgaaaggcg
    12181 gatttttttg ttgttggtgc caaagactac tcacctccgg cccacccagt tcgacaatac
    12241 gctcgcgaaa ctctgaactg gtcatggttt cacggcaagc catcatctgt tcaattaacg
    12301 gtaacgtgac accagaaatc acttcacaac gggagtgttt atggcttaat aatgaagcca
    12361 cgcggtacgg tggcgctccg gctaaatcgg ttaagaagat gacaccctca ccagagtccg
    12421 cttcatgcag agcatcgcac atcatgcggc tgagcatatt agagcttaat ccgcgccaga
    12481 aattcacggc ccggcactga agaagtgccc catgtttttt ctccagcgca tccagtatct
    12541 cttgcgcccg gtcatcgtga caggtaatta cccaacctaa catcgccttc ctccttagcc
    12601 gaaaatcagt ctaccctgac cattgttgat aaagggtgat agccgtcaaa actcctctcc
    12661 cccgaatcca gaggagagga aatagcctta gctacgcaaa ccacgtccac gttggatcag
    12721 cgaccaacag atcaaataaa acgccacaat aaagaccacc agtacgccaa aggtagtgac
    12781 cagcggcaca tcatttatac cgaggaagcc gtagcggaaa ccactgatca tataaacgat
    12841 tgggttcagg tgcgacagcc cttgccagaa cggcggcaac aaagtcaggg agtaaaacac
    12901 cccgcccaaa tacgtgagtg gcgttaacac aaaggttggc accaggctga tgtcatcgaa
    12961 cgttttggca aacacgccgt tcagcaaacc cgcaagggag aatagcaccg ccgtgagcac
    13021 cagggttaag gcaacgaata cccacgaatg cacctgaaac ggcacaaaaa acagtgaaat
    13081 tgccgtcacc agaatgccaa caaacagacc acgcgccacg ccaccgccga catatccggc
    13141 aataatgacg tgagtcggaa ccggcgctac cagcagctct tcaatattac gctggaactt
    13201 ggcaccaaaa aatgatgacg cgacgttagc gtaggcattg gtgatcaccg acatcatgat
    13261 cagccccggt acgatgaact gcatatagct gaagccatgc atatcgccaa tacgcgaacc
    13321 tatcaggtta ccgaagataa taaagtaaag ggtcatggtg atgactggcg gcaccagcgt
    13381 ctgcacccag atacgcataa agcgatggat ctctttcgcc cagatgcttt ttagcgccac
    13441 ccagtaaaga tgcatcatgc gcgatctcct tgtttttcat taaccagtga aacaaacagc
    13501 tcttccagac ggttagcttt gttacgcata cttaatacct gaatgccctg ctcacttaac
    13561 tgcgtaaata cgctgttgat cccctgctca cgcagcactt caacttccag cgtcgcggta
    13621 tcgaccagtc gatactgata gccatcgagc ttcggtaacg gacttttcgg tgcgagatcg
    13681 agaataaagg tttccgattt cagcttagcc agcagcgcct tcatcgaggt attttccacc
    13741 agctcaccgt gttgaataat gccgatattg cggcacagca tttctgcttc ttccaggtag
    13801 tgtgtggtga gaatgatggt ggtgcctttg tcgtttaaat ccttcaaaaa gccccacatt
    13861 gagcggcgaa gttcaatatc cacgcctgcg gtcggttcgt cgagaatcag tagtttaggt
    13921 tcatgcatta acgcacgggc aatcattaaa cggcgcttca tcccgccaga taacatacgc
    13981 gcacgttcgt tgcgttttcc ccatagatcg agctgcttaa gatatttttc gctgcggatg
    14041 tacgcctctt tgcgctccac accgtagtac cctgcctgat tcaccacaat ttgctgcacg
    14101 gtttcaaacg ggttgaagtt aaattcctgc ggcaccagtc ccaactgacg tttagcgttc
    14161 acgacatcct tctcgagatc gtaaccaaat acgctgaccc gcccggaggt tttatttacc
    14221 agagagctga taataccgat agtggtcgat ttcccggccc cgttcggccc gagaagcgca
    14281 taaaaatcac ccgcttcgac ctgcaaatct atcccacgaa gcgcctgaac gccgcctgga
    14341 taggtttttt taagctgttg aagttccagt gcaatggtca taaattttta cttaccttac
    14401 gttcttacac tttatctgtg gtttaaatcg tcccggagtt gccctatatt agccaaacgt
    14461 aattatttgg ttacaggtcg ttaacctcca tgaaagacat agatacactc atcagcaaca
    14521 atgcactatg gtcaaaaatg ctggtggaag aggatcccgg gttttttgag aaactggcac
    14581 aagcgcaaaa accgcgcttt ctatggattg gatgttccga cagtcgcgtt cctgcagaac
    14641 gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg taatgttgct aacctggtca
    14701 ttcacactga cctgaactgc ctttccgtgg ttcagtatgc agtggatgta ctcgaagttg
    14761 aacacattat tatctgtggc cactacggtt gcggcggcgt acaagccgca gttgaaaacc
    14821 cggaactggg gcttatcaac aactggctgc tgcatatccg cgatatctgg ttcaaacata
    14881 gctcattgct cggcgaaatg ccgcaagagc gccgtctgga taccttgtgt gaactgaacg
    14941 tcatggaaca ggtgtataac ctgggccact ccaccattat gcaatcagcg tggaaacgcg
    15001 ggcagaaagt taccattcac ggctgggcct acggcattca cgacggcttg ctgcgtgatc
    15061 tggatgttac cgccaccaac cgcgaaaccc ttgagcaacg ttaccgtcac gggatttcca
    15121 acctcaagct gaaacacgcc aaccacaaat aaaaatgcca tgccggatgc aacacatccg
    15181 gcaacttcac acttactcgt ccagcagaat cactttgccg atatacggca gatgacggta
    15241 acgctgtgcg taatcaatgc cgtaacccac cacaaactca tccgggatcg agaaaccgat
    15301 aaattctacc gggacgttca cttcacgacg ggacggttta tccagcagcg tacaaatcgc
    15361 cagcgacttc ggttcgcgca ggcttaagat ctcacgcact ttcgacagtg tattccccga
    15421 gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg atatcttcat ccagatcttt
    15481 gaggattttc acatcacggg tggtggacat gccgctaccg tagctggagg cggtcataaa
    15541 gtcgacttca tgagatacct gaacttcacg gcacaggtcc gccataaaca taaatgagcc
    15601 acgcagcaga cccaccagca ccatatcgct gccgctgtct ttgtaacgct cagtaatctg
    15661 acgacccagt tcggcgatac gcgctttaat ctccgcttcg gggatcatta cttctacagt
    15721 atgtttcata tctctaacca tatgatttaa aaacaaatca cttcgcacgg gcaggttttg
    15781 gcaccgcaac aaagcgcaag acacgcagta taccagtaaa actattcatt cgcggaatct
    15841 gttaataaag ctcgaattgt gatgacgatc acacatgtta aaacctataa ttaattgcta
    15901 ctaaaatatt aatgaattga aatggtgtct ctttatggca attaacaata caggctcgcg
    15961 acgattactc gtcacgctaa cagccctttt tgcagcgctt tgcgggctgt atctactcat
    16021 tggcggaggc tggctggtcg cgattggcgg ctcctggtac taccctatcg ctggccttgt
    16081 gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgt gccgcgcttt ggctatacgc
    16141 cgccctgctg ctcggcacca tgatttgggg cgtctgggaa gttggtttcg acttctgggc
    16201 gctgactccg cgcagcgata ttctggtctt cttcggcatc tggctgatcc tgccgtttgt
    16261 ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg tggcactgct
    16321 gattagcggt agtatcctga cctgggccgg atttaacgat ccgcaggaaa tcaacggcac
    16381 cttaagcgcc gatgccacac ctgcggaagc tatctcaccc gttgccgatc aggactggcc
    16441 tgcctatggt cgcaatcagg aaggtcaacg cttttcacct ctgaaacaaa ttaacgccga
    16501 taacgtccat aatctgaaag aagcctgggt gttccgtact ggcgatgtga agcagccgaa
    16561 cgatccgggc gaaatcacca atgaagtgac gccgattaaa gtgggcgaca ccctttacct
    16621 gtgtaccgct caccagcgcc tgtttgcgct cgatgccgcc agcggcaaag agaaatggca
    16681 ttacgatcct gagctgaaaa ctaacgagtc tttccagcac gtaacctgcc gtggcgtctc
    16741 ttatcatgaa gccaaagcag aaacggcttc gccggaagtg atggcggatt gcccgcgtcg
    16801 tatcattctt ccggtcaatg atggtcgact gattgcgatt aacgctgaaa acggcaaact
    16861 gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa agcaatatgc cagacaccaa
    16921 accgggtctg tatgaaccga cttcgccacc gattatcacc gataaaacca tcgtgatggc
    16981 aggttcagtt accgataact tctcaacccg cgaaacgtct ggcgtgatcc gtggttttga
    17041 tgtcaacacc ggggagctgc tgtgggcttt tgatcccggc gcgaaagatc cgaacgcaat
    17101 cccgtctgac gaacacacct ttacctttaa ctcgccaaac tcgtgggcgc cagcggccta
    17161 tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg accacgccgg atatctgggg
    17221 cggtaaccgc acaccggaac aggaacgtta tgccagctcg attctggcgc tgaatgccac
    17281 taccgggaaa ctggcgtgga gctaccagac cgttcaccac gacctgtggg acatggatct
    17341 tccggcacag ccgacgctgg cggacatcac cgttaatggt cagaaagtgc cagttattta
    17401 cgctccggcg aaaaccggca acatttttgt gctcgatcgt cgcaatggcg aactggtggt
    17461 tccggccccg gaaaaaccgg tgccgcaagg tgctgcgaaa ggcgattacg ttactccgac
    17521 ccagccgttc tctgaactga gcttccgtcc gaagaaagat ttgagcggtg cggatatgtg
    17581 gggagccacc atgtttgacc aactggtgtg ccgcgtgatg ttccaccaga tgcgctatga
    17641 aggcattttc acgccgccat ctgaacaggg cacgctggtc ttcccgggta acctgggaat
    17701 gtttgaatgg ggcgggattt ccgttgatcc aaatcgtgaa gtggcaattg ccaacccaat
    17761 ggcactgccg tttgtttcga aactgatccc acgcggtccg ggtaacccga tggagcagcc
    17821 gaaagatgcc aaaggcacgg gtacggaatc cggcattcag ccacagtacg gtgtaccgta
    17881 tggtgtcacg ctcaacccgt tcctctcacc gtttggtctg ccgtgtaaac agccagcatg
    17941 gggttatatc tcggcgctgg atctgaaaac caatgaagtg gtgtggaaga aacgtattgg
    18001 tacgccgcaa gacagtatgc cgttcccgat gcctgtaccg gtgccgttca atatgggtat
    18061 gccgatgctg ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg ctgctacggc
    18121 agataactac ctgcgcgctt acaacatgag caacggtgaa aaactgtggc agggccgttt
    18181 accagcgggt ggtcaggcga cgccaatgac ctatgaagtg aatggcaagc agtatgtggt
    18241 gatctccgca ggcggtcacg gttcatttgg tacgaagatg ggcgactata ttgtggctta
    18301 tgcgctgccg gatgatgtga aataaccata atgcccggag agatccgggc atatttccga
    18361 atacggtctt tttataccgt aaaccctaac atcatccccg tatcttcatg ctccagcaga
    18421 tggcagtgcg ccatataagc atgttctttc ggtgcgttgt gattaaactt caccagcact
    18481 tcactgacat tgccttctac cttaacggta tctttccagc ccgcgcgatg agccgctggc
    18541 ggtttgccat tttctgacaa gatacggaac tgggtgccgt gaatatggaa cggatgcagc
    18601 atcatgtcgc ccacgccaga gataacccaa cgttcgtact gccctttcgc cgccgcaaac
    18661 atcggcttat tcatatcaaa cgcctgaccg ttgattttat tggcatggtg gaaatcgaac
    18721 ttcccgccat ggttcatatg attcatattg ccgtgcccca tatggcccat catctggcta
    18781 tgatccatcc cggccatcgc ctgatcgcca tatttctcca tcagcatctg catccccatc
    18841 atatcgagca tcgggtccat agaaagttgc agcttgcgta ccgtcagccc ttccagcgaa
    18901 ggtaacgcag gcaggctact taatgtgtct ggcaaagcac cggaggcact aatagcaatc
    18961 ggctgaatcc gcattaccgg atgcggcttg tcaaacggcg caatcgccat ccccatctgg
    19021 ctgaccggta gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac cagcacttca
    19081 aaacgctcgc ccatcagcac cggcagctcg ctcaccttca ccggttcagg tagcagacca
    19141 ccgtcgctgg caatcacata cagcgggcga ttgtcgctgg tggcgaaatt gagcgagcgg
    19201 gcattacagc cattaagcaa acgcaggcgc agccaaccac gcggggcagc gtgttgtggg
    19261 tagattgcac cgttggtcag cagcgtatcg ccaaaccagc ccacggcggc ggtcatcaca
    19321 tccagttgat aatcaatctg cccatcagcg ttaaattttt tatcctgaac aatcaccgga
    19381 acatcatcga tgccccactg ttttggcagc attaatttca ggatctcgtc atcttcaatt
    19441 accaccagcc cagccaaccc catcgccacc tgtcgcccgg tcttgccatg ttgatgtgga
    19501 tggaaccagc aagtagcggc aggttgatca acgttcaacg tcaccgagcg cttgccacct
    19561 ggcagaataa ttccctgcgg gccgccgtcg acttcacccg gtacttccag cccgtgccag
    19621 tgcaacgtcg tctcttccgt cagttggtta tagatatcaa ccgttaccgc tttgccgcgc
    19681 tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt tgcagttttc
    19741 tcgccaaagg tagactgacc tgcgccaata gttaactgaa tgcgattacg ggcatcggtc
    19801 gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaagactgc gcggctccac
    19861 agcggcaagg ctgaagccac acccagcgcg acggaatatt ttaagaaatc acgacgttgc
    19921 atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc atagtccttc
    19981 cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt cgtcgcctcg
    20041 ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg aagacgtttt
    20101 tcagaacagt gttattcggc agcctgatgg ccgtttgcgc aaacagttac gcgctcagcg
    20161 agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg aagaacgatt
    20221 gtggttacca gaacttacct aacgggcaaa ttcgtcgcgc actggtcttt ttcgcccagc
    20281 aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagccctc ggtgaagaca
    20341 gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaagtgcaaa gccctggccc
    20401 gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt tgtagaaata
    20461 ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt tacgggtgtt
    20521 aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg ctacacgacc
    20581 agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc gatcaccagg
    20641 atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat ggcgtagtac
    20701 aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt ccgctactgg
    20761 cccacggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc atgctgcgtg
    20821 aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat gcgggtgtcg
    20881 tgtcgttctg ccgtcagtat ctgcccaacc ataacgccgg tagctacgac gatccgcgct
    20941 ttaagctggt gatcgacgat ggcgtcaatt tcgttaatca aaccagccag acctttgatg
    21001 tcattatctc cgactgcacc gatcctatcg gtcccggcga aagtctgttc acctcagcat
    21061 tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca caaaacggcg
    21121 tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc cattacttca
    21181 gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggtatc atgacttttg
    21241 catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt caggcgcgtt
    21301 ttctcgcttc tggcctgaaa tgccgttatt acaatccggc aatccatacg gcagcttttg
    21361 ccttacctca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga gaagataaga
    21421 aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg agtttttgta
    21481 tttacgatat ctgctacgcc aaaactgccg aagagcgcga cggttatatt gcttatatcg
    21541 atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt tccattatcg
    21601 gggccaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc agcgtcacta
    21661 ttctggtgag tgaagaaccg gttgacccga aactcatcga caaaacagaa caccccggcc
    21721 cactgccaga aacggtcgtt gcccatctcg ataaaagtca tatttgcgta catacctacc
    21781 cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa gtctctacct
    21841 gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt gagtccgata
    21901 tcgtaaccat tgattatcgc gtgcgcggtt ttacccgcga cattaacggt atgaagcact
    21961 ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg aaggcgctgt
    22021 atgacatggt ggatgtgaac gtctatcagg aaaatatctt ccataccaag atgttgctta
    22081 aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta accgacagcg
    22141 agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat tacgggcgca
    22201 atatgccagc tgtttaacgg ctctggcgga gctcccaggc tccgccagat ttatttactt
    22261 ctgctgcacg aaattgcggt aagccgccac gacctgcaga aaatcctcaa cgccgcatag
    22321 cgacaggctt tcttcgtcgt agtagttcat cccctcttcc atttcatcgc cagcgaattc
    22381 cagttgattg gcgcgaacca tcacctcttc accgtccatc cacagggtgt attcatgccc
    22441 tgcccgttgc caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt ccacttcatc
    22501 aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgacca cttcatgccc
    22561 catggacata cgcaccttta ccactccggt aatatcgcgc agaaattcgt aatccatagt
    22621 gtgttcctct gctcccggca atgcgccgaa atcgcattgc gccattgatg taattatcgc
    22681 agccttaccg gagaaaaaca gcggaacggc gatggtttag aaataaaaaa tgcccgggaa
    22741 tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt attacaccgc
    22801 agtctggaaa atcaccccat cggctttttc ggtgtactga gaaagctggt tgaagttcag
    22861 ataacgataa gtatcaacgg cagttttatc tacttgcgca acgtaggtct ggtactcttc
    22921 cggcgtcggc agtttgccaa tcagcgccgc aacagccgcc aattctgcag aagccaggaa
    22981 gacattcgcg ccagtcccca gacggttcgg gaagttacgg gtagaggtgg aaaccaccgt
    23041 cgcaccgtct gctacacgcg cctggttacc catacacagg gaacagccag ggatctcgat
    23101 acgcgcaccg ctcttaccga agacgctata gtagccttct tcggtcaact gcgcggcatc
    23161 catacgggtt ggcggtgcca cccacaggcg cgtcggcaac tggcctttgt gcgcatccag
    23221 cagtttaccc gcagcacgga agtgaccgat gttggtcatg caggaaccga taaacacttc
    23281 gtcgatcttc tcgccctgta ccgcagacag cggacgtgcg tcgtccgggt cgttcggtgc
    23341 acagaggatt ggctctttaa tatccgccag atcgatgtcg atcactgccg cgtattccgc
    23401 atctgcatcg gcttccagca gctcaggatt cgccagccat ttttccatgc cctgaatacg
    23461 acgttccagg gtacgacgat cgccgtaacc ttccgcgatc atccacttca gcaggacgat
    23521 gttagagttc aggtattcga tgatcggttc tttgttcagc ttgatggtac aaccagcggc
    23581 agaacgctcg gcggacgcat cggtcagttc aaatgcctgc tcaactttca gatccggcag
    23641 accttcaatt tccagaatgc gaccagagaa gatgtttttc ttgcctttct tctcaacggt
    23701 cagcagacct tgtttgatcg catacagcgg gatcgcgtgt accagatcgc gcagggtgat
    23761 gcccggctgc attttgcctt tgaagcgcac cagaacggat tccggcatat ccagcggcat
    23821 tacgcctgtt gcggctgcaa atgccaccag accagaaccc gccgggaaag agataccgat
    23881 cgggaaacgg gtatgggagt caccaccggt accgacggta tccggcagca gcatacggtt
    23941 cagccaggag tggattacgc cgtcacccgg acgcagcgac acaccaccac ggttcataat
    24001 gaagtccggc agcgtgtggt gcgtgttcac gtcaactggc ttcggatacg ccgcggtatg
    24061 gcagaaagac tgcatcacca ggtcagccga gaagcccagg cacgccaggt ctttcagttc
    24121 atcacgggtc atcgggccgg tggtgtcctg tgaacctaca gaagtcattt tcggttcgca
    24181 gtacgcgccc ggacgaatac ctttcacacc gcaggcacga cctaccattt tctgcgccag
    24241 cgagaagccg cgatcgctct cagcgacatc tttcgcctga cggaacacat cactgtgcgg
    24301 cagaccaagt gcttcacgcg ctttggtggt caggccacgc ccgataatca gcgggatacg
    24361 gccgccagca cgcacttcat caatcagcac gtcggttttc agttcgaagg tcgccagcag
    24421 ttcgccggtt tcgtggttac gcacttcacc tttgtacggg taaacgtcaa tcacgtcgcc
    24481 catgttcagg ttagagacgt cgacttcgat tggcagtgca cccgcgtctt ccatcgtgtt
    24541 aaagaagatg ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt tgttcggcac
    24601 atgtggaata tcatcgccca taaaccacag aacggagttc gtggcggatt tacgcgatga
    24661 acccgtaccc acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt gcagagcttc
    24721 aatctgtttg atcgggccaa caacacctgg ctggtctggc tcaatacctt cacgggcgtt
    24781 tttcagcatc gccagcgcgt gcagtgggat atccgggcgc gaccacgcat ccggtgccgg
    24841 agaaaggtca tcggtgttag tttcgccagt gactttgaag acagtaacgg tcagtttttc
    24901 agccagcgcc gggcgattca ggaaccattc ggcatccgcc caggactgca taacctgctt
    24961 cgcatattcg ttgcctgctt tcgctttctc ttctacgtca tagaagttat cgaacatcag
    25021 cagcgtgtga gaaagtgctt tggcagcaat aggtgccagt ttggcatcat ccagcgcgtc
    25081 gatcagcgaa tgaatgttgt aaccaccctg catggtgccc agcagttcga tggctttttc
    25141 cggagtcagc agaggggatt tggcttcgcc tttcgcgata gcagccagga agcctgcttt
    25201 gacataggcg gcttcatcga cgcctggggg aacacggttg gttaacagat ctaacaggaa
    25261 ttcttcttcg cccgcgggcg ggtttttcag cagctctaca agtgcggcca tttggtttgc
    25321 atccaggggt ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct tacggtattc
    25381 ttctagcacg acggttctcc tcgctctcat tgtcatagtg cggcaggcga ttctcttcac
    25441 gctcctgtga gacagcagtt tgtagggtaa atgcccagta ccgcaacggg cagaatagca
    25501 ggattttgac gatgtgttaa tctgtttaca aaaaagcaac ataaaaaagt ggctgaatcg
    25561 ttaaggatgg tctagagatt gtttccctcc gtaaaaactt cgttaattcg catggtgata
    25621 atcacctttc tcaacgaatc ccaagattag tcaaaattta aactaccgcc tctttatact
    25681 cggattcaca gcacctgcgg gtggcagttc gccgaccatt gcgatttcct tgagatccga
    25741 attatgaaaa tgactttgcc gtttaaaccc catgtgctgg cgctaatttg cagtgccggg
    25801 ctttgtgccg cctctaccgg gctatatata aaaagccgca cagtggaagc gcctgtggaa
    25861 ccgcaatcga cacaacagac tgcgcctgac atcaccgcag ttacgcttcc tgcaacggtt
    25921 tccgcaccgc ccgtaacgcc cgccgtcgtc aaatccgcat tcagcactgc acaaatcgat
    25981 caatgggttg cgcctgtcgc gctgtacccc gattctctgc tttcacaagt gttaatggca
    26041 tcaacctatc cggcaaacgt tgctcaagca gtgcaatggt cgcacgataa tccacttaaa
    26101 caaggcgatg ctgctattca ggcggtatct gaccagccgt gggacgccag cgttaaatca
    26161 ctggtggcct ttccacaatt gatggcattg atgggcgaaa acccgcaatg ggtgcaaaac
    26221 ctgggcgatg cttttctggc gcagccgcag gacgtgatgg actcggtaca gcgattgcgg
    26281 caactggcgc aacaaaccgg ttcgctgaag tcatcaaccg aacaaaaagt tattaccaca
    26341 acgaagaaag ctgtaccggt aaaacagaca gtcacggcac ccgtcatacc atccaatacc
    26401 gttttaactg ccagccccgt cattacagag cctgcaacaa ccgtcatttc cattgagccc
    26461 gccaatcctg atgtggtcta tattcccaac tacaacccaa ccgtggttta cgggaactgg
    26521 gccaatactg cgtatccgcc ggtttatctg ccaccaccag ccggagaacc gtttattgac
    26581 agctttgtgc gcggattcgg ctatagcatg ggtgttgcta ccacgtacgc actattcagc
    26641 agcatcgact gggatgacga cgatcatgac catcatcatc atgacgatga tgattatcat
    26701 caccacgatg gcggtcatcg tgacggtaat ggctggcaac acaacggcga caacatcaat
    26761 atcgacgtca acaatttcaa ccgtatcacc ggtgagcatc ttactgataa gaatatggca
    26821 tggcggcaca atccaaacta ccgtaatggt gtgccctatc atgatcagga tatggcaaag
    26881 cggtttcatc aaaccgatgt caacggcgga atgagcgcca cgcagctacc tgctccaaca
    26941 cgcgacagcc agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca cgccgccccc
    27001 gtcattacac gagataccca acgtcaggca gcggcacagc ggtttaatga agctgaaaac
    27061 tatgggagct atgacgactt ccgcgacttc agccgtcgcc aaccactgac ccagcaacaa
    27121 aaggacgccg ctcgtcagcg ttatcagtcg gcctcgcctg agcagcgcca ggcagtccgc
    27181 gagaaaatgc agactaaccc acagatccag cagcgaagag acgcagcgcg tgagcgtatt
    27241 cagtcagcct cgcctgagca gcgccaggtg tttaaggaaa aagtacagca gcgcccactg
    27301 aaccaacagc aacgtgataa cgcccgccag cgtgttcaat cagcatcacc tgaacaacgt
    27361 caggtttttc gggaaaaagt tcaggagagc cgcccacaac gtctaaacga cagtaaccat
    27421 actgtcaggc tgaataacga gcaacggtca gcagtacgcg aacgtctctc tgagcgcgga
    27481 gcaaggcgac aggaaaggta aattgcagac gtaaaaaaag cggcgtggtt agccgctttt
    27541 ttaattgccg gatgttccgg caaacgaaca attacttctt cttcgctttc gggttcggca
    27601 ggtcggtaat gctaccttcg aacacttctg ccgccaggcc cacagactcg tgcagagtcg
    27661 ggtgcgcgtg gatggtcagt gcgatgtctt cagcatcaca acccatttcg attgccaggc
    27721 cgatttcacc cagcagctcg ccgccgttgg taccgacaat cgcaccaccg atcacacggt
    27781 gagattcttt gtcgaaaatc agcttggtca taccgtctgc gcagtcggaa gcgatagcac
    27841 gaccagaagc agcccacggg aaggtggcgg tttcatagct gatgcctttc tctttcgctt
    27901 ctttctcagt cagacctacc catgcaactt ctggttcggt ataggcgatg gacgggataa
    27961 ctttcggatc gaagtagtgt ttcttaccgg cgataacttc agcggcaacg tgaccttcgt
    28021 gaacaccttt gtgtgccagc atcggctgac cgacgatatc gccgatagca aagatgtgcg
    28081 gtacgttggt acgcagctgt ttgtcaacgc ggatgaaacc acggtcgtcc acttccacgc
    28141 cagctttgcc tgcgtcgagg tttttaccgt tcggcacacg accaatcgct accagcacgg
    28201 cgtcgtaacg ctgcggttca gcgggtgctt ttttgccttc catcgtcaca taaataccgt
    28261 cttctttcgc ttcaacggcg gtaactttgg tttccagcat caggttgaat ttcttgctga
    28321 tacgcttggt gaagacttta acgatgtctt tgtcagctgc cgggataacc tggtcgaaca
    28381 tttcaaccac gtcaatctgt gaacccagcg cgtggtatac ggtgcccatt tccagaccga
    28441 tgataccgcc acccattacc agcaggcgtt ctggtacttc tttcagttcc agcgcgtcag
    28501 tggagtccca gatacgcgga tcttcatgcg gaataaacgg cagttggatc gggcgagaac
    28561 ccgctgcaat gatcgcattg tcgaagttga tcacggtttt gccgttctca ccttcaactt
    28621 ccagggtgtt agccccggtg aatttaccca gaccgttgac cactttgact ttgcggcctt
    28681 tcgccatacc agccagacca ccggtcagct gattgatcac tttctctttc caggtacgaa
    28741 tcttgtcaat atcggttttc ggttcgccga agacgatacc gtgttcagcc agcgctttgg
    28801 cttcttcgat aacttttgct acgtgcagca gtgctttaga agggatacag ccgacgttca
    28861 ggcaaacacc gccaagggtg ttgtaacgtt ctacgattac ggtttccaga cctaaatcag
    28921 cgcaacggaa ggcagcggag taacctgcgg ggcctgcccc aagtaccacg acctgagttt
    28981 tgatttcagt actcatcatg acctctatat atttatctcc ggcggtcata cccgtcgtct
    29041 ttcaggccgc agcggcgtta gcttcacgcc ctcaccccag tcacttactt atgtaagctc
    29101 ctggggattc tggcgcttgc taccttgctg cacccagaaa tccatagggt atgtgaagta
    29161 ataccctaac caccaccggg tcgttctatc cgtcggtatt ttacaaaatt gttaacaatt
    29221 tttaaacaac aaacggcaac cgatttgtct attcgctaat aacctcaata cattcatgag
    29281 attaccagaa aaaagccggc cgttgggccg gctcttttac ttacatcacc agacggcgaa
    29341 tgtcagacag cgtgttgtta atgatggtaa tgaaacgggc accatcagca ccgtcgatca
    29401 cgcggtggtc gaaggagaga gaaatcggca gcatcagacg cggcacgaac tctttaccat
    29461 tccacaccgg ctccatcgcg gacttggaaa cgccgaggat agccacttcc ggcgcgttca
    29521 caatcggcgc gaagtgggta gtacccaggc cgccgatgct ggagatggtg aagcaaccgc
    29581 cctgcatttc gcccgcagtc agcttaccgt cacgcgcttt cttagaaata gtcatcagct
    29641 cgcgagacag ctcgatgatg cctttcttgt tgacgtcttt gaataccgga acaaccagac
    29701 cgttcggggt atccaccgcc acaccgatgt tgatgtattt cttcagggtc agacgctgac
    29761 cgtcttccga caacgaactg ttgaagcgag gcatctgctc aagagctgca gcaacggctt
    29821 tcatgatgaa gacaaccggg gtgatcttca catccagctt acgtttcgcc gcttcttcgt
    29881 tctgctgttt acggaacgct tccagctcgg tgatatcggt tttgtcgaag tgagtaacat
    29941 gcgggatcat cacccagtta cggctcaggt tcgcaccaga aattttctgg atacggccca
    30001 gttccacttc ttcgatttca ccaaacttgc tgaagtccac cttcggccac ggcagcatgc
    30061 ctgggatacc gccgccagtc gccgccggag ctgcttctgc acgtttgata gcttctttca
    30121 cgtaagcctg aacgtcttcg cgcaggatac gacctttacg gccagtgccc ttcactttcg
    30181 ccaggttaac gccaaactcg cgtgccagac ggcggatcag cggagtcgcg tgaacgtaag
    30241 cgtcgttttc tgcaaattca gatttgcctt ccgctttcgc agccggtgct gctgccggag
    30301 cttcagcttt tgctgccgga gccggcgctg ccgcttcctg tttcgcagga gccgccgcag
    30361 gcgctgcgcc ttcaacttcg aagatcataa tcagcgagcc agttttcact ttatcgccaa
    30421 cgttgacttt cagttccttc acgacgcctg caaacggagc cggaacttcc atagaagctt
    30481 tgtcgccttc tacggtaatc agtgactgtt cagcggcaac tttgtcgccc actttcacca
    30541 tcacttcggt cacttcaact tcgtcaccgc cgatatccgg aacgttaact tctttcacgc
    30601 cagccgctgg tgcaggggcc gctgccggag ccgcttcctg tttagccgcc ggagctgccg
    30661 cgcctgcttc acccgcgact tcgaagacca taatcagcga gccggtagac actttgtcac
    30721 ccacgttcac tttgatctct ttcacggtgc cagcaaacgg agccggaact tccatagacg
    30781 ctttatcgcc ttctacggtg atcagcgact gttcagcttc aactttatcg ccaactttca
    30841 ccaggatttc ggtcacttca acttcgtcgc tgccgatatc cggaacgtta acgtcttttg
    30901 ccgctgcagc cgctggtgct gctgccggag ctgcttcttt cttctcttct gcctgagcag
    30961 gtgcagcgtc tgctgcaccg tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg
    31021 ttttatcgcc aacagagact ttgatctctt taacgatacc cgcctgcgga gacggaactt
    31081 ccatagaggc tttgtcgcct tctacggtga tcagcgactg ttcggcttca actttgtcgc
    31141 ccactttgac caggatctcg gtgatttcaa cttcatcagc cccgatgtcc ggtactttga
    31201 tttcgatagc cattattctt ttacctctta cgccagacgc gggttaactt tatctgcatc
    31261 gatgttgaat ttggcgattg cgtcagcaac cactttctta tcgatttcgc cacgtttagc
    31321 cagttcgccc agcgccgcta caaccacgta ggaagcatca acttcgaagt ggtgacgcag
    31381 gttctcacgg ctgtcggaac gaccgaagcc atcagtaccc agtacgcggt agtcgtcagc
    31441 cggtacgtaa gtacggacct gctcagcgaa cagtttcata tagtcggtag atgccactgc
    31501 cggagcgtcg ttcatcacct gagcgatata cggaacgcgc ggagtttcca gcgggtgcag
    31561 catgttccag cgttcacaat cctgaccatc tcgcgccagt tcggtgaagg aggtcacgct
    31621 ataaacgtca gaacctacgc cgtaatcttt cgccaggatc tctgccgctt cacggacgtg
    31681 acgcaggata gaaccggagc ccagcagctg aactttacct ttgctacctt caatggtttc
    31741 gagtttgtag atacctttac ggataccttc ctcagcacct tccggcattg ccggcatgtg
    31801 gtagttttcg ttcagcgtgg tgatgtagta gtaaacgttc tcttgttttt caccgtacat
    31861 acgctccaga ccgtcatgca taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga
    31921 gatacagttc ggaatagtca gcgactgaat gtggctgtga ccatcttcgt gctgcagacc
    31981 ttcgccgttc agggtggtac gaccggaagt accgccgatc aggaagccac gcgcttgctg
    32041 gtcgccagcc gcccagcaca gatcgccaat acgctggaag ccgaacatcg agtaatagat
    32101 gtagaacggg atcatcggca gattgttggt gctgtaagag gtcgccgctg ccagccagga
    32161 acaacctgcg cccagctcgt tgatcccttc ctgcagaatc tgacctttct cgtcttcttt
    32221 atagtaagca acctgctcgc ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat
    32281 accaatctga cggaacagac cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg
    32341 taccagacga tctttgatcg acttgttctt cagcattacg ttcagggcac gaacgaaagc
    32401 gatagtggta gagatctctt tgctctgctc ttccagcagc gcgccgaagt cttgcaggct
    32461 cggcagctca agcttctcgg tgaagttcgg ctgacggctt ggcagataac cgtgcagttt
    32521 ctgacgctga gcgtgcagat aggtatgctc ttcagaacct tccgggaagg tgatgtacgg
    32581 cagtttttcg atatctgcat cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac
    32641 gccgtccatg ttcattttct taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc
    32701 catgccgtaa cctttaatgg tatgagcaag gattactgtc gctttgcctt tggtttcctg
    32761 cgctttcttg aatgcagcgt agattttctt cggatcgtga ccaccacggt tcagtgccca
    32821 gatctgctcg tcagtccagt ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa
    32881 gtgttcacga acgtacgcac catctttcga tttgaaggtc tggtagtcgc cgtcaacggt
    32941 ttcgttcatc agctggatca gtttaccgct ggtatcttta cgcagcagtt catcccaacg
    33001 gctaccccac atcactttga tcacgttcca gccagcacct tcgaagatgc cttccagttc
    33061 gttgatgatc ttgccgttac cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat
    33121 aacgaagact aggttatcca gtttttcacg ggtagcgatg gtgatcgcac ctttggattc
    33181 cggttcgtcc atttcaccgt cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt
    33241 caggccacgg tgttccagat atttcaggaa tttagcctgg taaatagcac caatcggccc
    33301 cagacccata gatacggtcg ggaactgcca gaattccggc atcagtttcg ggtgcggata
    33361 ggaagagagg ccattgccgt gaacttcctg acggaagtta tccagctgct cctgagtcag
    33421 acgaccttct aggaaagcac gagcgtacac gcccggggag atgtggccct ggaagtaaac
    33481 caggtcgccg ccatcctgct cgttgcgtgc acggaagaag tggttaaagc acacatcata
    33541 aatggttgcg gaagactgga aggacgccat atggccgccc agttcgaggt cttttttcga
    33601 cgcacgcagc accgtcatga tggcgttcca gcggatagct gaacgaatac ggcgttccag
    33661 ttccagatta cccggatact ccggttgttc ttcaacgggg atggtgttga tgtagttgct
    33721 gatacctgtg cctgcggcta cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc
    33781 gatcagatac tgagcacgct caacaccttc ttcacggatg accgattcga tcgcctggag
    33841 ccagtcgcga gtttcgatcg gatccacgtc atttgggaaa cgttctgaca tgggttattc
    33901 cttatctatc taataacgtt gagttttctg gaacctgtcc cattgaactc tcgccggaaa
    33961 gctcaataag acaggttcta cgtttagttg ccgcgctttt atatgcgctt gatttacaac
    34021 atcttctgga taatttttac cagaaaaatc actaattctt tcgttgctcc agacgacgca
    34081 gagaacgctc acggcggctc tcttcacgac ttctgtcgag caaaatttct tcgataaagg
    34141 ccagatggcg atgcgatgct tcgcgcgctt cttccggctt accggccata atcgcttcaa
    34201 atatgcgggt gcggtgacta ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt
    34261 cgaagttctg gcggacattc tgggccaaca tcggctccat acaccttagc agatgaagca
    34321 gaaccacatt gtgggccgct tcagtgacag caatctgata ctggagtacg gcgtttgatt
    34381 ccgcgtccag atcgccagac tgctgcgcca gctctatggc gtggtggagt tcacggatgc
    34441 gttccttgtc ttcatcggta ctacgcagcg cggcgtaata agcggcgata ccttccaggg
    34501 cgtgtcgtgt ttcgagcaag tcatactgtg actcaggatg gtcggagagc agctccacca
    34561 gcggatcgct gaagctttgc cataggctgc tctggacaaa agtgccgcca ccctggcgac
    34621 gaagcaacaa gcccttcgct tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga
    34681 cgtcaaactg ttttgccagt tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc
    34741 cttcgaggat caaaaactcc agttgctgct caatcacatc ggagagtttt ggttggcgga
    34801 ttttgctgta ggccatgagt tcctgtctta agccacttgc cgaagtcaat tggtcttacc
    34861 aatttcatgt ctgtgacgct aaagtaacaa agtattcacc ttatgtccat acaggttttg
    34921 attgaaatca tgaaactgtg cacattttaa caacttgaca tatataacgt ttcaaagttg
    34981 taactatgca caaatgtaga ctttacgtag gaaaggagtt ttttaacgat tattaaacta
    35041 taaaaatccg aattgaaccg attcacttac caattttgtg atttttaatt caattaaaac
    35101 gaatttaaat tcattctaca tattgagagg ggttgaggct gagctttaca aacggtttct
    35161 ttttaagcaa ctcatcttca accatgcata aagcgggtgc attcgctgcc tcataccatt
    35221 attcttgatc tgacggaagt ctttttgtaa caattcaaac ttctttgatg taaacaaatt
    35281 aatacaacaa acggaattgc aaacttacac acgcatcact gcgtagatca aaaaaacaac
    35341 caccgcacga ggtttcatga tggaaggtca acagcacggc gagcagctaa agcgcggcct
    35401 taaaaaccgc catattcagc ttatcgcgct gggtggcgcg atagggaccg ggttattcct
    35461 gggtagcgcc tccgtaatac agtccgcagg gccagggatt atcctgggtt acgccattgc
    35521 tggttttatc gcctttctga tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt
    35581 cgcaggctcc tttagccact ttgcttataa atactggggc agttttgccg gtttcgcctc
    35641 tggctggaac tactgggtac tgtacgtttt agttgccatg gctgagctga ctgccgtggg
    35701 taaatacatt cagttctggt atccggaaat ccccacctgg gtttctgccg ccgtattctt
    35761 tgtggtgatt aacgccatca acctgaccaa cgttaaagtg tttggcgaga tggagttctg
    35821 gtttgccatt atcaaagtta tcgcggtggt agcgatgatc atcttcggcg gctggctgct
    35881 attcagtggc aacggcggcc cgcaggcgac cgttagcaac ctgtgggatc agggtggttt
    35941 cctgccgcac ggcttcaccg ggctggtgat gatgatggcg atgatcatgt tctcgttcgg
    36001 tggtctggaa ctggtgggga tcaccgcagc agaagctgat aacccggagc aaagtatacc
    36061 gaaagcaact aaccaggtta tctaccgcat cctgattttc tatattggtt cgttagccgt
    36121 tctgctctca ctgatgccgt ggacccgcgt taccgccgat accagtccgt ttgtgctgat
    36181 cttccacgag ttaggcgata cctttgtggc gaatgcgctg aacatcgtgg tactgactgc
    36241 ggcgctctcc gtgtacaaca gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc
    36301 acaacagggt aatgcgccaa aagcgctggc gtctgtcgat aaacgtggtg taccagtaaa
    36361 taccattctg gtgtctgcac tggtaacggc gttgtgcgta ctgattaact accttgcccc
    36421 agagtccgct ttcggactgt taatggcgct ggtggtatct gcactggtaa tcaactgggc
    36481 gatgattagc ctggcgcata tgaaattccg tcgcgccaag caggaacaag gcgtggtaac
    36541 tcgcttccct gctctgcttt atccgctggg taactggatc tgcctgctgt ttatggcggc
    36601 ggtactggtg attatgctga tgaccccagg aatggcgatt tcggtatacc tgatcccggt
    36661 atggctgatc gtgttaggta tcggctatct gtttaaagag aaaaccgcca aagccgtaaa
    36721 agcgcattaa tctctctacg ccctcacccg tacagggtga gggcaataat ctttacacca
    36781 acgccccgta aatcgtcagt agtgcaatca ccaccacgac cacgaacgag gttttcttcg
    36841 ccattgaaac cgctgccctc ggcgtctcca ccttatcgac atgcggttca cgcgccagag
    36901 agaactgcgc cagacgcgtt aacacctgat actgcgaagt atggaaatca cccagcgaag
    36961 caaaccaggc cggtaacgct ttctcaccat gaccgatcaa ggcatatacc acacccgcaa
    37021 gacgaaccgg cacccaatcc agtacatgaa gcacggcatc aatgccggac tgtaaacgat
    37081 gatgcggcgt ctgatatcgc gccagccagt attgccatgc acgcaaaaac gcatacccca
    37141 tcagcgtaac gggtccccag gttcccccca caatcagcca gaacagcggt gcaagataaa
    37201 aacgaaagtt aatccacagc aatgcatttt gcagctcacg caaatactca cgttcgtcgc
    37261 agcctgccgg gacgccgtga atcatggtga gttcgccagc catcgtggca cgggcatggc
    37321 tatcattacg tgaagcagct gtcagataag catgataatg aagacgaact ttacctgcgc
    37381 caatacacag caaaccaatc agcagccaca ccagtagcgt gggaacgttg aacaatactc
    37441 cctgcaatgc gcgtaacagt aaaaaagtca cgcccatcgc aataatggtc atgcctaacg
    37501 tgcgcccgag agaaaaatgt ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct
    37561 gccagtgctc gcccaactta aacaggcgct cgaaaattaa caccagtaag gttgtaaata
    37621 gcgtcatgtt gtctccttgc tgaccagcac acgaaaccgt gcccaatcaa atgcaggacc
    37681 gggatcggtt ttccgatccg gcgcaatatc acaatggccc gtcatgtttt tagcgatatc
    37741 cggatagcaa tcaatcagtg cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt
    37801 atacgccagc gtatcggtgc cttcaagctc aatcccaata gaaaaatcat tgcagcgttc
    37861 gcgcccctga tactgagaga ctcccgcatg ccatgcacgt ttatcgaaag gaacatactg
    37921 gactatttca ccatcacggc gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc
    37981 agcaaagaaa ggatgtgcct gcggatcaat agttccagtg aataatgcgt cgatccacgg
    38041 accgccaaac tcgcctggcg gcaggctaat attgtgcacc accagcaggg tgggtgtttc
    38101 gtcatccggg cggcaatcgt aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc
    38161 ctgttctaac aacatgcagg atctccttat atgtggtgct aatacccggt tcagagtagc
    38221 atgtttctac cttatgattc gttagctatc tggagtttta acatgccgcc tcgccgctat
    38281 aaccctgaca cccgacgtga cgagctgctg gaacgcatta atctcgatat ccccggcgcg
    38341 gtagcccagg cgctgcggga agatttaggc ggaacagtcg atgccaacaa tgatattacg
    38401 gcaaaacttt taccggaaaa ttctcgctct catgccacgg tgatcacccg cgagaatggc
    38461 gtcttttgcg gcaaacgctg ggttgaagag gtgtttattc aactggcagg cgacgatgtc
    38521 accataatct ggcatgtgga tgacggcgat gtcatcaatg ccaatcaatc cttgttcgaa
    38581 cttgaaggcc catcccgcgt gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa
    38641 accctttcag gagttgccag taaggtacgc cactatgtcg aattgctgga aggcaccaac
    38701 acgcagttgt tggatacgcg caaaacctta cccggcctgc gttcagctct gaaatacgcg
    38761 gtactttgcg gcggcggagc gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa
    38821 gaaaaccata ttattgcctc cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg
    38881 cacccggatg cgccagtaga agtcgaagta gagaatctgg aagaacttga tgaagccctg
    38941 aaagcaggag ccgatatcat catgctggat aacttcgaaa cagaacagat gcgcgaagcc
    39001 gtcaaacgca ccaacggcaa ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca
    39061 ctgcgtgaat ttgccgaaac gggcgtggac tttatctccg tcggtgcgct aactaaacac
    39121 gtacaagcac tcgacctttc aatgcgtttt cgctaatagt tgacagattt atccgctcca
    39181 tcgcgggcgg ataatctccc cttccccaac tttcttcgta acgcctcgca aatttcttgt
    39241 ctttcgctgc aacgaaataa aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc
    39301 tcgcctgtgc tgatggcgtt tgccaaagta gcgccaacta aatcaaggag cgaaacagat
    39361 ggacaagcaa cgcggtttta cacttatcga actgatggtg gttattggca tcattgccat
    39421 tttaagcgcc attggtattc ccgcttatca aaactacctg cgcaaagccg cactcaccga
    39481 catgctacaa acctttgtgc cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg
    39541 tggattagat acctgcgacg gtggcagcaa tggcattccc tcgcctacca ccacccgcta
    39601 tgtttcagcc atgagtgtgg caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa
    39661 tgggctaagc gtcgtcatga caccgggttg ggataacgca aacggcgtca ccggctggac
    39721 gcgcaactgc aatattcaaa gtgacagcgc attgcatcaa gcctgcgaag atgtcttccg
    39781 ctttgatgac gccaactaag gagcggcaat gaatattcca cagctcactg ccctgtgtct
    39841 gcgttatcat ggagtcttgc tggatgccag cgaagaggtg gttcatgttg cggtagtcga
    39901 tgcaccttcg catgagctac tggacgcatt gcatttcgct accaccaaac gtattgagat
    39961 cacctgctgg acgcgccaac aaatggaagg tcacgccagt cgcacacaac agacattgcc
    40021 cgtagctgtt caggagaagc atcagcccaa agcagagttg ctaactcgaa cgttacaatc
    40081 tgcgctggaa caacgcgcgt ctgatattca tatcgaacca gcggacaatg cctaccgcat
    40141 ccgcttgcgt atcgacggcg tattgcatcc tttaccggat gtttcaccgg atgccggagt
    40201 cgcattaacc gccagattaa aagtgctggg aaacctggat attgcggaac atcgcctgcc
    40261 gcaggacggg caattcactg tcgaactggc aggaaacgcc gtctcatttc gtattgcgac
    40321 cttaccatgt cggggtggtg aaaaggtggt attaaggttg ttacagcagg tgagccaggc
    40381 actggatgtc aacacgcttg gaatgcagcc gttacaactg gcggactttg ctcatgcctt
    40441 gcaacaacca cagggactgg tgctggtaac tggccctaca ggcagcggca aaacggtcac
    40501 gctttatagt gccctgcaaa cgctgaatac cgctgacatt aatatttgta gcgtcgaaga
    40561 tccggttgag atccccatag ccggactaaa ccagacgcaa atccatccgc gtgccggact
    40621 cacctttcag ggcgtgttgc gtgcgttatt gcgccaggat cctgacgtca tcatgatcgg
    40681 agagatccgc gatggcgaaa cagcagagat cgctattaaa gcggcgcaaa ctggtcacct
    40741 ggtgttgtct accctacaca ctaattccac ctgcgaaacg ctggtacgtt tacagcaaat
    40801 gggggtcgcc cgctggatgc tatcatcggc gcttacgctg gtaatagccc agcgtctggt
    40861 acgcaaactt tgcccacatt gtcgccggca gcaaggggag cccatccaca ttccagacaa
    40921 tgtatggcca tcgccgctgc cccactggca ggcacccggt tgtgtacatt gctaccacgg
    40981 tttttatggt cgtacggcct tatttgaagt tctgcccata acgccggtca ttcgtcagct
    41041 tatttccgct aataccgacg ttgaatcgct ggaaacgcac gcacgacagg cgggtatgcg
    41101 tacgcttttt gaaaacggct gcctggccgt ggagcaaggc ttaaccacct ttgaagagtt
    41161 aatccgcgta ctggggatgc cgcatggcga gtaagcaact ctggcgctgg catggcataa
    41221 ccggcgacgg caatgcgcaa gatgggatgc tatgggcaga gagccgtgct ttgctgctca
    41281 tggcactaca gcaacagatg gttaccccac ttagcctgaa gcgaatcgcc atcaattctg
    41341 cgcagtggcg aggagataaa agcgcggaag tcattcatca actggcgacg ctactcaaag
    41401 ccgggttaac gctttctgaa gggctggcac tgctggcgga acagcatccc agtaagcaat
    41461 ggcaagcgtt gctgcaatcg ctggcgcacg atctcgaaca gggcattgct ttttccaatg
    41521 ccttattacc ctggtcagag gtatttccgc cactctatca ggcgatgatc cgcacgggtg
    41581 aactgaccgg taagctggat gaatgctgct ttgaactggc gcgtcagcaa aaagcccagc
    41641 gtcagttgac cgacaaagtg aaatcagcgt tacgttatcc catcatcatt ttagcgatgg
    41701 caatcatggt ggttgtggca atgctgcatt ttgttctgcc ggagtttgcc gctatctata
    41761 agaccttcaa caccccacta ccggcactaa cgcaggggat catgacgctg gcagacttta
    41821 gtggcgaatg gagctggctg ctggtgttgt tcggctttct gctggcgata gccaataagt
    41881 tgctgatgcg ccgaccgacc tggcttatag tgcggcagaa attgctgtta cgcatcccga
    41941 ttatgggttc actgatgcgg ggacaaaaac tcacgcagat ttttacgatt ctggcgctga
    42001 cacaaagtgc aggcattact tttttgcagg gcgtagagag cgtcagagaa acaatgcgct
    42061 gcccgtactg ggtgcaactt ctgacacaaa tccagcacga tatcagtaac ggtcatccca
    42121 tctggctggc gctaaaaaat accggggagt ttagcccgct ctgtttgcaa ttagtgagaa
    42181 caggagaggc atccggctcg ctggacctca tgttagacaa cctcgcccat catcatcggg
    42241 ataacacaat ggcgctggcg gataacctcg cagccttact ggaaccggcg ttgttgatca
    42301 taacgggagg aattatcggt acgctggtgg tggcgatgta tctgccaatt ttccatttag
    42361 gcgatgcgat gagtgggatg ggataacgct ggcgtgttgc tccacaccag cgttggaaga
    42421 ttacaggttg ttgaagatgc ggttttcttg ttcctgcaca cggataaatg tggtgcgctt
    42481 ggtcagttct ttcaggcgtg aagccccaac gtatgtacaa gctgaacgca ggccgcccaa
    42541 aatatcacgc gcggtatttt caaccgggcc tcgcagcggc agcttaacgg ttttaccttc
    42601 tgctgcgcga tattccgcaa cgccgccaac gtgacgtttc atcgcagact cggagctcat
    42661 gccgtagaac agcataaatt tctcgccgtt ctcctcaacg atgcgaccgc cgctctcttc
    42721 gtggcccgcc agcatgccgc caagcatgac gaaatcggca ccgccgccaa aggcttttgc
    42781 gacatcgccc ggcgtggtgc agccaccgtc gctgacgatc attccgccca gaccgtgcgc
    42841 cgcatcggca cattcgatta ccgcagaaag ctgcggataa ccgacgcctg ttttgacgcg
    42901 agttgtacaa acagaacctg ggccaatgcc aactttaacg atatcagcac cggagaggat
    42961 aagctcctca cacatttcac cagtcactac gttgccagca caaatggttt tggtcggcca
    43021 cgcttcacgc gctttcgcaa cgaactgcac gaagtgttcg gaataaccat tcgccacatc
    43081 aatacaaacg aagtttaatg ccgggttcag gtcgagaatc tgtttagttt tttcgaaatc
    43141 cgcatcagac gtaccggtag aaaccatcac atgtttcagc acatcagcgg aagaattgtt
    43201 gataaacgct tgccactctt cgacagaata gtgtttatgc acagcagtca aaatatcaaa
    43261 agaagccagc gcagaggcca tagaaaatgt acctacggtg tccatatttg cggcgataat
    43321 tggcacgccg gaccagctct gacctgaatg tttgaaggtg aattgacgtt ccagttcaac
    43381 atcggaacgg cttttaagag tggagcgttt agggcggatg agaacgtctt taaaacctaa
    43441 cttcagatct tcttcaatac gcatgtgcgg attcctgggg ttaatggcta taagggtaag
    43501 agcgcaactc cagtgacgct atcatacgca ctaatcaatg cggcgcaaga ctgcgaaatt
    43561 gtcttttttt acgctacaat cccataaatt tacctggtaa atagtgagcg aacgtttcat
    43621 caatttcgct ttcgtgcact catttttaac gttttgataa tcatgatgaa ttcccgggaa
    43681 tataaattat gaggtatata gttgccttaa cgggaggcat tggcagtggc aagagtaccg
    43741 ttgccaatgc gtttgctgat ctcggaatta acgtcattga tgccgatatt attgcgcgtc
    43801 aggtggttga accaggtgca cctgcgctac atgccattgc tgatcacttt ggcgctaaca
    43861 tgattgctgc tgatggaaca ttgcagcgcc gggccttgcg cgagcggatc ttcgccaacc
    43921 cggaagagaa aaactggctt aacgccctgc tgcatccgct gattcagcaa gagacgcaac
    43981 accagatcca gcaagctact tccccctatg tactgtgggt tgtgccattg ctggtagaaa
    44041 actcactgta taaaaaagcg aatcgagtgc tggtggtgga tgtcagccca gaaacgcaac
    44101 ttaagcgcac catgcagcgc gatgatgtaa ctcgcgagca tgtcgaacaa atccttgctg
    44161 ctcaggcaac gcgcgaagcc cgccttgccg tggcagatga cgtcattgat aataacggcg
    44221 caccggatgc tatcgcatcg gatgttgccc gcctgcacgc acactatttg cagcttgcgt
    44281 cgcagtttgt ctcacaggaa aaaccgtaat gcagacccag gtcctttttg aacatccact
    44341 aaatgaaaaa atgcgtacat ggctgcgcat tgagtttttg attcagcaac tcaccgttaa
    44401 tttacccatc gttgaccacg ctggcgcgct gcatttcttc cgtaatgtca gtgaattact
    44461 ggatgttttc gagcgcggcg aagtccgcac tgagctgttg aaagaacttg accggcagca
    44521 acgtaaactc cagacctgga ttggcgtgcc tggcgtggac cagagccgta ttgaagcatt
    44581 aattcagcag ttaaaagcgg cggggagcgt attaatttcc gcgccgcgta tcgggcaatt
    44641 tctgcgtgaa gatcgtttga ttgctctggt gcgtcagcga ctgagcatcc caggcggctg
    44701 ttgcagcttt gatttaccta cattgcacat ttggctgcat ctaccccagg cgcagcgcga
    44761 cagccaggta gaaacctgga ttgccagcct gaacccgctc acccaggcac ttaccatggt
    44821 gctggattta attcgccagt cggctccctt ccgtaaacaa accagcctga atggttttta
    44881 tcaggataac ggtggcgatg ccgacttgct gcgcctgaat ctgtcgctcg attcacagct
    44941 ttatccgcaa atttccggac ataagagccg ttttgccatt cgttttatgc cgctggacac
    45001 tgaaaacgga caggtaccgg aacgtctgga tttcgaactg gcctgttgct aaggagtgaa
    45061 aatgtcagaa actattacgg tgaattgccc aacctgcggg aaaacggtgg tgtggggtga
    45121 aatcagcccg tttcggccat tttgctccaa acgttgtcag ctgatcgacc tcggagaatg
    45181 ggctgctgaa gaaaaacgaa tccccagcag tggcgatctt tccgaaagcg atgactggag
    45241 cgaagagcca aagcagtgac atttgtctga tgcc
//
LOCUS       DAZGLJ010000014        44800 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000014 DAZGLJ010000000
VERSION     DAZGLJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.014"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(303..845)
                     /gene="ycfP"
                     /locus_tag="ACHK49_000700"
     CDS             complement(303..845)
                     /gene="ycfP"
                     /locus_tag="ACHK49_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO9261390.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(868..1893)
                     /gene="nagZ"
                     /locus_tag="ACHK49_000701"
     CDS             complement(868..1893)
                     /gene="nagZ"
                     /locus_tag="ACHK49_000701"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO9261391.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(1904..2728)
                     /gene="thiK"
                     /locus_tag="ACHK49_000702"
     CDS             complement(1904..2728)
                     /gene="thiK"
                     /locus_tag="ACHK49_000702"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO9261392.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2709..3350)
                     /gene="lpoB"
                     /locus_tag="ACHK49_000703"
     CDS             complement(2709..3350)
                     /gene="lpoB"
                     /locus_tag="ACHK49_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO9261393.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3364..3741)
                     /locus_tag="ACHK49_000704"
     CDS             complement(3364..3741)
                     /locus_tag="ACHK49_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO9261394.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3744..4103)
                     /gene="hinT"
                     /locus_tag="ACHK49_000705"
     CDS             complement(3744..4103)
                     /gene="hinT"
                     /locus_tag="ACHK49_000705"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO9261395.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4437..6626
                     /gene="fhuE"
                     /locus_tag="ACHK49_000706"
     CDS             4437..6626
                     /gene="fhuE"
                     /locus_tag="ACHK49_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO9261396.1"
                     /translation="MLSTQFNRDNQYQASTKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQHIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLIGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HIKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(6686..8119)
                     /gene="ptsG"
                     /locus_tag="ACHK49_000707"
     CDS             complement(6686..8119)
                     /gene="ptsG"
                     /locus_tag="ACHK49_000707"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO9261397.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8414..9211)
                     /locus_tag="ACHK49_000708"
     CDS             complement(8414..9211)
                     /locus_tag="ACHK49_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO9261398.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9222..10226)
                     /gene="holB"
                     /locus_tag="ACHK49_000709"
     CDS             complement(9222..10226)
                     /gene="holB"
                     /locus_tag="ACHK49_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO9261399.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10223..10864)
                     /gene="tmk"
                     /locus_tag="ACHK49_000710"
     CDS             complement(10223..10864)
                     /gene="tmk"
                     /locus_tag="ACHK49_000710"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO9261400.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10854..11876)
                     /gene="yceG"
                     /locus_tag="ACHK49_000711"
     CDS             complement(10854..11876)
                     /gene="yceG"
                     /locus_tag="ACHK49_000711"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO9261401.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11879..12688)
                     /gene="pabC"
                     /locus_tag="ACHK49_000712"
     CDS             complement(11879..12688)
                     /gene="pabC"
                     /locus_tag="ACHK49_000712"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO9261402.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12808..14049)
                     /gene="fabF"
                     /locus_tag="ACHK49_000713"
     CDS             complement(12808..14049)
                     /gene="fabF"
                     /locus_tag="ACHK49_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO9261403.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14137..14373)
                     /gene="acpP"
                     /locus_tag="ACHK49_000714"
     CDS             complement(14137..14373)
                     /gene="acpP"
                     /locus_tag="ACHK49_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO9261404.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14584..15318)
                     /gene="fabG"
                     /locus_tag="ACHK49_000715"
     CDS             complement(14584..15318)
                     /gene="fabG"
                     /locus_tag="ACHK49_000715"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO9261405.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNEIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15331..16260)
                     /gene="fabD"
                     /locus_tag="ACHK49_000716"
     CDS             complement(15331..16260)
                     /gene="fabD"
                     /locus_tag="ACHK49_000716"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO9261406.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLIASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVINFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16276..17229)
                     /gene="fabH"
                     /locus_tag="ACHK49_000717"
     CDS             complement(16276..17229)
                     /gene="fabH"
                     /locus_tag="ACHK49_000717"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO9261407.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17297..18367)
                     /gene="plsX"
                     /locus_tag="ACHK49_000718"
     CDS             complement(17297..18367)
                     /gene="plsX"
                     /locus_tag="ACHK49_000718"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO9261408.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18448..18621)
                     /gene="rpmF"
                     /locus_tag="ACHK49_000719"
     CDS             complement(18448..18621)
                     /gene="rpmF"
                     /locus_tag="ACHK49_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO9261409.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18673..19194)
                     /gene="yceD"
                     /locus_tag="ACHK49_000720"
     CDS             complement(18673..19194)
                     /gene="yceD"
                     /locus_tag="ACHK49_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO9261410.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19393..19977
                     /gene="yceF"
                     /locus_tag="ACHK49_000721"
     CDS             19393..19977
                     /gene="yceF"
                     /locus_tag="ACHK49_000721"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO9261411.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20089..21048)
                     /gene="rluC"
                     /locus_tag="ACHK49_000722"
     CDS             complement(20089..21048)
                     /gene="rluC"
                     /locus_tag="ACHK49_000722"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO9261412.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21621..24806
                     /gene="rne"
                     /locus_tag="ACHK49_000723"
     CDS             21621..24806
                     /gene="rne"
                     /locus_tag="ACHK49_000723"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO9261413.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(25002..25955)
                     /gene="flgL"
                     /locus_tag="ACHK49_000724"
     CDS             complement(25002..25955)
                     /gene="flgL"
                     /locus_tag="ACHK49_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO9261414.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     RQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(25967..27610)
                     /gene="flgK"
                     /locus_tag="ACHK49_000725"
     CDS             complement(25967..27610)
                     /gene="flgK"
                     /locus_tag="ACHK49_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO9261415.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNTGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27676..28617)
                     /gene="flgJ"
                     /locus_tag="ACHK49_000726"
     CDS             complement(27676..28617)
                     /gene="flgJ"
                     /locus_tag="ACHK49_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO9261416.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28617..29714)
                     /gene="flgI"
                     /locus_tag="ACHK49_000727"
     CDS             complement(28617..29714)
                     /gene="flgI"
                     /locus_tag="ACHK49_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO9261417.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29726..30424)
                     /gene="flgH"
                     /locus_tag="ACHK49_000728"
     CDS             complement(29726..30424)
                     /gene="flgH"
                     /locus_tag="ACHK49_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO9261418.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30477..31259)
                     /gene="flgG"
                     /locus_tag="ACHK49_000729"
     CDS             complement(30477..31259)
                     /gene="flgG"
                     /locus_tag="ACHK49_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO9261419.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31397..32152)
                     /gene="flgF"
                     /locus_tag="ACHK49_000730"
     CDS             complement(31397..32152)
                     /gene="flgF"
                     /locus_tag="ACHK49_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO9261420.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32172..33377)
                     /gene="flgE"
                     /locus_tag="ACHK49_000731"
     CDS             complement(32172..33377)
                     /gene="flgE"
                     /locus_tag="ACHK49_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO9261421.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(33402..34097)
                     /gene="flgD"
                     /locus_tag="ACHK49_000732"
     CDS             complement(33402..34097)
                     /gene="flgD"
                     /locus_tag="ACHK49_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO9261422.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34109..34513)
                     /gene="flgC"
                     /locus_tag="ACHK49_000733"
     CDS             complement(34109..34513)
                     /gene="flgC"
                     /locus_tag="ACHK49_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO9261423.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34517..34933)
                     /gene="flgB"
                     /locus_tag="ACHK49_000734"
     CDS             complement(34517..34933)
                     /gene="flgB"
                     /locus_tag="ACHK49_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO9261424.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35088..35747
                     /gene="flgA"
                     /locus_tag="ACHK49_000735"
     CDS             35088..35747
                     /gene="flgA"
                     /locus_tag="ACHK49_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO9261425.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            35823..36116
                     /gene="flgM"
                     /locus_tag="ACHK49_000736"
     CDS             35823..36116
                     /gene="flgM"
                     /locus_tag="ACHK49_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO9261426.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36121..36537
                     /gene="flgN"
                     /locus_tag="ACHK49_000737"
     CDS             36121..36537
                     /gene="flgN"
                     /locus_tag="ACHK49_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO9261427.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(36577..38112)
                     /gene="murJ"
                     /locus_tag="ACHK49_000738"
     CDS             complement(36577..38112)
                     /gene="murJ"
                     /locus_tag="ACHK49_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO9261428.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGTMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVM
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38222..39145)
                     /locus_tag="ACHK49_000739"
     CDS             complement(38222..39145)
                     /locus_tag="ACHK49_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO9261429.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTVLWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39147..39794)
                     /locus_tag="ACHK49_000740"
     CDS             complement(39147..39794)
                     /locus_tag="ACHK49_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO9261430.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39805..40389)
                     /gene="rimJ"
                     /locus_tag="ACHK49_000741"
     CDS             complement(39805..40389)
                     /gene="rimJ"
                     /locus_tag="ACHK49_000741"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO9261431.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40625..41833
                     /gene="mdtH"
                     /locus_tag="ACHK49_000742"
     CDS             40625..41833
                     /gene="mdtH"
                     /locus_tag="ACHK49_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO9261432.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41897..42544
                     /gene="grxB"
                     /locus_tag="ACHK49_000743"
     CDS             41897..42544
                     /gene="grxB"
                     /locus_tag="ACHK49_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO9261433.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPPRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNLADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42678..43238
                     /locus_tag="ACHK49_000744"
     CDS             42678..43238
                     /locus_tag="ACHK49_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO9261434.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43344..44390
                     /gene="pyrC"
                     /locus_tag="ACHK49_000745"
     CDS             43344..44390
                     /gene="pyrC"
                     /locus_tag="ACHK49_000745"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO9261435.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44464..44709
                     /gene="dinI"
                     /locus_tag="ACHK49_000746"
     CDS             44464..44709
                     /gene="dinI"
                     /locus_tag="ACHK49_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO9261436.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        10827 a        12126 c        11155 g        10692 t
ORIGIN      
        1 attgagaata acatgaatgg tgcattggtc ataccaaaat tgatgttaat caagttttgt
       61 tgctgaaaag ataggcgact ttaccgggtg agatcaaaaa acagagttta ttgtttatta
      121 tttgagcaag tccggaaggg cgatataggg tgaagcgtat atcgagggcg tttggtcgtc
      181 ctgataagag gcgcatcagg cattgatgct cgaatgtcgg atgcggcgtg aacgccttat
      241 ccgacccaca gaatccggag cgcatcaggt accggcaagg ttacctgatg cgacgagggc
      301 atttacccga gggttttgaa cgctttaatg cgctgtaaat gcggggagat attcttgaat
      361 ttgtgcgtct gctcttcgtc ccagacaatc tcgtaataat gatgcagctc ttcagatgtc
      421 cgctggctgt taagcgcttc atcattacgc gacaaaatca ccaggcaacg atcgcgattc
      481 ttctcacgga agttggtcac acacttagtg gcaatatcgg catactcttc cgggcgatca
      541 atcttgcctt ccatgttctc ataagggaac aaattagggt tgaagatcac ctggcggata
      601 tcgcaaagaa aaccaatccg ttccgcccag tatccgccca gaccaacgcc gcaaatcagc
      661 ggacgctcgt cgacgttcag ttgcaacatt ttgtccactt ctttgagcag atgctgcata
      721 tcatgtttcg gatgccgcgt actgtagctt atcaagcgta catccgggtc aataaactgc
      781 aattgtaaga ctttctcgtg gttacccgga ctgttagagt caaaaccgtg taaatagata
      841 atcatcgctt cctcacataa gccagggtta gtgacctgct ttctcttcct gccagcgttc
      901 atgtaactga ttcagacggg tgctgatcgc tttccagcga gccgagtcca tcagttcctg
      961 tcgcgaaaat gaacctttat gatacaaacg tgtaacacgt tctgccttga tcggtgacag
     1021 attatctaac acgctgacgg cccctttacg attattgcag accaggatca tatcgcaacc
     1081 cgcatccagt gatgcctgcc cgcgttcggc ataactgccc ataatcgcgg caccttccat
     1141 cgataaatcg tcagagaaaa tcacgccgtc aaaacccagt tcctgacgca aaacggtttt
     1201 cagccagtag ggagaaccgc tcgccggacg cggatcaaca tcactgtaga tcacatgcgc
     1261 aggcataatg gcgtcgagtt tattttcgcg gattaaggaa ctgaagaccg acatatcttt
     1321 agcgcgaatc tccgcttgtg gacgtggatc gcacggtgtt tctttgtgtg agtctgccgt
     1381 tactgcaccg tgtcctggga agtgtttccc ggtcgttttc attccggctt catgcatacc
     1441 atcaataaac cggctggcaa ttgccagggc tttttgtgga tcggcatgat aagaacgctc
     1501 gccaatcgcc gcgctgatat gcccgacatc cagcactggc gcaaagctga tatcaatatc
     1561 catagcgatc atttcgctgg ccatcagcca acccgcctct tgcgccagtt tgccgccctc
     1621 ttccattcct gacagcgcag cgaatgattg cgccgctggc aagcgggtaa aaccttcacg
     1681 aaaacgctgc acgcgtccac cttcctgatc taccgccacc accagatgat tgcgcgaagc
     1741 tgcgcggatc tggcgcacca gttcacgcaa ctgggcagga tcatgatagt tacgcgtaaa
     1801 gagaatcagc cctcccacca gcggatgcgc aagtatttca cgctcttccg cgtccagttc
     1861 gtaaccttcg acatccaaca ttactggacc cacactgctc tccttattgt tttattaata
     1921 gctgccgcca ggtgtcatcg gccagcctga taaattgttg atcgccggtt tgtcgccagc
     1981 ggtactcaaa ccaccctgct ttgagcatca gcagccaggg aaaccatcgc ctgacctgac
     2041 gccataattg cgccggataa atcttcgcgc gagtggcata gtcattgacc aattgccggt
     2101 gctgttcagt attttccacc cacactgccg ccagttccag cgcgatatca ccatctccgg
     2161 catactccca gtcgatgagt tttaaccctg acgcgctatg cactaaattt ccggcgtgga
     2221 catccatatg caatggactt aagcgtaaag gccgtggttc ccgcgctttg cgcagacgtt
     2281 ttaacattcg cagccaaccc actgtccgcc gcgccggatc gctttgctgc cagtacagtt
     2341 ccagtaacgg caacagcgtt attcgccagc caaaacgtgg ttgttgatgt agataataca
     2401 gcaagcctgc cagttcgttg gtatccggca aatacgtttt tacctcgccg ggcagatagt
     2461 cgactaccat ccagtcacgg agatataaat gcggcttcgg tgcaatgctt gcgggtagtt
     2521 gtgataaagc ccgatactgg cgcaagaacg cggactgcgg cgcatcagga tcgtgcggct
     2581 gacgcacaac aaaacgctgg ccctgatgtt caatgagaaa actcccgcca ctaagcccac
     2641 tattaaacgt cgtgacggga tgaaactgcg ggaaaaagcg cgacagcaat tcgtcgcgca
     2701 tgatgggatt attgctgcga aacggcacct ttacctgacc agataatttc gcccgtctgc
     2761 accagcatca gctgcatttg tagggtcgga gcgttaacgt tgccagaggc gctggagtac
     2821 agcacgtaat gagcgccgac attgcgggca atgcctatgg ctttactacg ggtacctaaa
     2881 ctgtcctgcg gcgacaaacc taactgttgc ttagccatcg acagctgctg ggcggaaacc
     2941 agggtaaatt tcccgttatt agccagcgca tttcgcagcg tttcggtcgc ttctgcggca
     3001 ttcagcgaac cgttagtacg gttgttaacg ctatcaacca gcaggacgct acccgcagtc
     3061 accccgtcag ccccaagcat cttactgacc atcggctgca ttgcgccatt ccagtcataa
     3121 tggcgaatat gcggcgcagg cggtgccgtt tgatcttcgt gctcaattgg gcctggctgc
     3181 tgcgggatcg tcggcaccga gggcactgtg gggacaggct gttgtggctc ggctggttgt
     3241 tccggcgctg gtttcacttc ttctaccggt gcaggttcac gttgccccac acacccggcg
     3301 agaaacatcg ccagcgcggt aatcaaggcg tagcgactca tttttgtcat caagattcac
     3361 cccttacaaa tatagataaa gtctgacttt gtgcgccccc agaaaattgg cgctgccgta
     3421 cagcgttacc gccgaatgtg cgggaatggt aacgctgcgt ggcctttcca gaggatgcat
     3481 ctccagccct ctggcgtcat accagtaaaa acgataatga acggtaacgg gttcttgcct
     3541 ttcgttataa agcgttgagg atgctgaagg ttgaatatca gacgtcgaaa ggacgggctt
     3601 ttctgcactg atgcccgcag ccaataaaga tgactccatc accagcgatt gctcatcatt
     3661 caccggaatt tccggatgtg aacgacagcc caccagcagt aacaacgcca gagacaccag
     3721 cccaaagcat ccttttctca tcgttacaga cctttatgtg ccagcattgg tcccagcgga
     3781 cggccaccca acaagtgcat atggatgtgg taaacctctt gtccgccatg gcggttggta
     3841 ttcatgatca gacgatagcc atcttcggca ataccttctt gctcagcaat ttttgccgct
     3901 acggtgatca tgcgtcccag cgcctgctca tgctcagctg acacgtcgtt cacagtcgga
     3961 atgaggatat tcggaatgat cagaatatgc gttggcgctt gcggcgaaat atcgcgaaac
     4021 gccgttacca gatcatcctg gtagacgata tcggagggga tctcacgacg aataattttg
     4081 ctgaatatag tttcttctgc cacgacattt tcctttttca aatagccatg cgttgcgcca
     4141 tgctacgccg cgccacactg cgagtataga gtatggtcga gttaaacctg ctctttcaac
     4201 cttaactcac gatttcttaa gcaaaaaatc gttttgctgc cgtatatatc gccattattc
     4261 ccatttctgc ctgcgaatgc gctgaaaaga cattctctgt ttggtttaca cgtcaaatat
     4321 ggatgcgtat atttctcatt tgcatttaca tacaaaatta ttcgcacact aaatataacc
     4381 gctcatcacc ctcctccggc atgagcctga cgacaacata aaccaagaga tttcaaatgc
     4441 tttcaacaca atttaacagg gataatcaat atcaagccag caccaaacca tcactacttg
     4501 ccggttgcat agcattggca ctattacctt ctgccgcttt tgccgctcca gccactgaag
     4561 aaacggtgat tgttgagggt tcagccacag ctccagatga tggcgaaaat gattacagcg
     4621 taacgtctac ctctgcgggt accaaaatgc agatgactca acgtgatatt cctcagtcgg
     4681 tcactattgt tagccagcag cggatggaag atcagcagtt acaaacgctg ggcgaagtga
     4741 tggaaaacac gctggggatc agcaaaagtc aggcggattc cgatcgtgct ctttattatt
     4801 cccgcggatt ccagatcgat aactatatgg ttgatggtat ccccacctat tttgaatcgc
     4861 gctggaatct gggcgacgca ctttctgata tggcactctt tgaacgcgta gaagtagtgc
     4921 gtggcgcgac aggactcatg accgggacgg gtaatccatc tgcggcaatt aatatggttc
     4981 gaaaacacgc gaccagtcgt gaatttaaag gcgatgtctc ggcggaatac ggtagctgga
     5041 acaaagaacg gtatgtggcg gatttacaaa gcccactcac cgaagacggt aaaatccgcg
     5101 cgcgaattgt cggcggctac cagaataacg actcatggct ggaccgctac aacagtgaaa
     5161 agaccttctt ctccggcatt gtcgatgctg atttaggcga tcttacgacg ctttcagccg
     5221 gttacgaata tcagcacatt gatgttaata gcccaacctg gggcggttta ccgcgctgga
     5281 atactgatgg cagcagcaac agttacgatc gcgcacgcag taccgcacct gactgggcgt
     5341 acaacgataa agagatcaac aaggtcttta tgaccctgaa gcagcggttt gctgatacct
     5401 ggcaagcgac gctgaatgcc acccactctg aagtcgaatt tgacagcaaa atgatgtatg
     5461 tcgatgccta tgtaaacaaa gcggatggta tgctgattgg gccatacagt aattatggac
     5521 ctggctttga ttatgtcggc ggcaccggtt ggaacagtgg caaacgtaaa gttgatgcgc
     5581 tggatttgtt cgctgacggt agttatgaat tgtttggtcg tcagcacaat ctaatgtttg
     5641 gtggcagtta cagcaaacaa aacaatcgtt acttcagttc atgggccaac atcttcccgg
     5701 atgaaattgg cagtttctac aactttaatg gcaatttccc acaaaccgac tggtcaccac
     5761 agagcctggc gcaggacgat accacacata ttaaatcgtt atatgctgcc actcgtgtca
     5821 cccttgccga tccgctgcat ctgatcctcg gcgcacgtta taccaactgg cgggttgata
     5881 cgctgactta cagcatggag aaaaaccaca ccacgcctta cgctggtctg gtgtttgaca
     5941 tcaatgacaa ctggtcgacc tacgccagct atacctctat tttccagccg caaaatgatc
     6001 gtgacagttc aggcaaatat ctggctccaa tcaccggtaa caactacgag ctgggtctga
     6061 aatcggactg gatgaatagc cgtctgacca ccacgttagc catcttccgt attgagcagg
     6121 ataatgtcgc tcagtccacc ggtacaccta tccccggcag caacggcgaa accgcctata
     6181 aagcagtgga tgggacagtc agtaaagggg tggaatttga actcaacggc gcaattaccg
     6241 acaactggca gctgacattt ggcgcaacgc gctatattgc agaggataac gaaggaaacg
     6301 ccgttaatcc taatctgcca cgcaccacgg ttaaaatgtt caccagctat cggttgcctg
     6361 tcatgccaga gttgacagtc ggcggtggtg ttaactggca aaatcgcgtg tataccgaca
     6421 ccgtgacacc gtatggcacc ttccgcgccg agcaaggtag ctacgcgctg gtggatctct
     6481 tcacccgcta ccaggtgacg aaaaacttct cgttacaggg gaacgtcaat aacctgttcg
     6541 acaaaaccta cgataccaac gtggaaggtt ctatcgtcta cggcgcaccg cgtaatttca
     6601 gcattaccgg cacgtatcaa ttctgaatag cacctgtaaa aaaggcagcc atctggctgc
     6661 cttagtctcc ccaacgtctt acagattagt ggttacggat gtactcatcc atctcggttt
     6721 tcaggttgtc ggatttagta ccgaaaatcg cctgaacacc agaaccagca accactacgc
     6781 ccgctgcgcc cagtttcttc aggccagcct gatccacttt agacacatca gcaacgctga
     6841 cgcgcagacg ggtaatacat gcgtcgaggt tagtaatgtt ttctttacca ccaaatgcag
     6901 caaccagagc cggtgccatt tcgctggtac ctgtcgcttt tgcatcttca gtcgcgtctt
     6961 cacgacccgg cgttttcaga tccagtgctt taatcagcac gcggaagatg gtgtagtaaa
     7021 caatcgcata accgataccg acgatcggga acagccacag tttgctgctg ttaccagaca
     7081 gaacgatgaa gtcgatcaga ccgtgcgaga acgacgtacc gtcacgcatc cccagaagaa
     7141 tacagattgg gaatgccagg cctgccagaa tcgcgtggat gatgtacagg atcggcgcaa
     7201 cgaacatgaa ggagaactcg atcggctcgg tgataccggt caggaacgag gtcagcgccg
     7261 cggagatcat aataccgccc actttcgcgc ggttttctgg tttagcagag tgccagatag
     7321 caattgcggc agctggcaga ccgtacattt tgaacaggaa gccaccagac agtttacccg
     7381 cagtcgggtc acccgccata taacgcggaa tgtcgccgtg gaaaacctga cctgctgcgt
     7441 tggtgtattc accaatctgc atctggaaag gtacgttcca gatgtggtgc agaccaaacg
     7501 gtaccaggca acgttcgatg aaaccgtaaa tgccaaacgc aactaccggg ttctggtaag
     7561 cagcccactg agagaaggtc tggattgcag aaccaatcgg cggccaaatg aaggacagca
     7621 caacgccagt aaagatggca gccaggccag aaatgatcgg cacaaagcgt ttaccggcaa
     7681 agaagccaag atactcaggc agcttaatac ggtagaaacg gttaaacatg tacgctgcga
     7741 tcgcaccgga gataatccct ccgagtacgc cagtatccgc caggtgttta gaggcgattt
     7801 cttcagcagg taaatgcagt accagtggcg caaccacggc catggtttta accatgatgc
     7861 cataggcaac aactgcggcc agcgcggata cgccatcgtt attggtaaag ccgagggcga
     7921 caccgatcgc aaaaatcagt ggcatgtttg caaagacgga accgcctgct tctgccataa
     7981 catgcgatac aacggcgggc agccagctga aattcgcgga accgacgccc agcagaatac
     8041 ctgcgatagg cagtacggat accggcagca tcagcgattt accgaccttt tgcaggttag
     8101 caaatgcatt cttaaacata attgagagtg ctcctgagta tgggtgcttt ttttacgttc
     8161 tcacgcgtgg caagggggga gagcctcgcc gtgtacaggg catctaagcg ccctttattt
     8221 attacacaga gtaaaataat tcagtgccaa tttgtttgac ggctatcacg tttcaacttc
     8281 agatcattgg agaagttgca cattttgaca aaacatatgt cacaatattt caaacgtggt
     8341 tattcaccgc ccaattttcg gcggtgaact ttactcgttt tagccattaa ttacgagctt
     8401 taaaaaaact cattcaacgg atggattgaa ggcgggaagc gtcgatgtga aacagacggg
     8461 cgaagttatc ggtggttacc tgcgccagtt cttcaacggc aacacctttc aacacagcca
     8521 tgtattctgc aacgtcacga accatcgcag gttgattctc ttttcctcga tgcggtaccg
     8581 gcgcaaggta aggtgagtca gtttccacca gtaatcgatc cagggggaca taacgcgcag
     8641 catcgcgcag ttgctccgca ttacggaagg tcacaatgcc ggaaaaggag atgtaaaatc
     8701 cgagatccag taatttaccc gccgtttctc tgtcctctgt aaaacagtgt agtacgccac
     8761 cgcaatccgt cactttttct tcgcgcagaa ttgccagcgt atcggcacgg gcgtcacggg
     8821 tatggacgat aaccggcttg ttcagttcac gaccaatctg gatatgatgg atgaaggact
     8881 cttgctgacg tactttagtt tccggtgtgt aataataatc cagcccggtt tcacccagcg
     8941 ctacaacacc ctcttctgcc gccagacggc gtaaatcttc tacatcgtag ggatcattct
     9001 ggttgagtgg atgcacgcca caagaaaata cgacgttgtc acgttcgcct accagatccc
     9061 gcatatgtaa ataacccggt aatgttgtgg cgactgccag acaaaatttc acatcgcgtg
     9121 cggcggcttt cgccagaacg tcatccacgt ccttatgcaa agattcataa tccagaccat
     9181 cgagatggca gtgtgagtcg actaaaaaca tgatgtctct cttaaagatg aggaaccggt
     9241 agcacaacgc ccggttgcag gtaatgctca atacgcagta aaagatcggt gatgagaagc
     9301 tcgcggttga tgcctgtaac agacattaac tgttcacgaa tgtggcaaac atcccccagt
     9361 atagcctgca ggcgcgaggg agaaagatgg tttgccagtt cggcgaccag gcccggcaca
     9421 tcaacattgg tcacctgcgc agcaccatga tggcgtttta gcgcatccat cagcaacgtt
     9481 gccagccagt gtaaacgcgc cggagcttgt tcatgattaa gggccgctag cagcgaatac
     9541 cagtcgcccg atggcacgct atatgccaac gcctgacaca atgtttcacg agcctgccag
     9601 ttatctccct gaaacaacgc cagtgccgcg ccaggcgaac cggcgcttaa gcgcaatgcg
     9661 gcaagtaatg catcctgtga cattgtcact tcgcgtgaaa gccaggtcac ggcgtactgt
     9721 tccggcggcg gcgcaaggta atgtaaccga caacgactac gtaatgttgc cagtaaacgt
     9781 tcaggctcgc gggtagccag gaaaaaccag gtttctgctg gtggctcttc aagcgttttc
     9841 agcaatgcgt tagccgcggc gtcggtcagt aaggcagcat cggttaccca gacaactttc
     9901 gcaccaccta ggcgtgcgtg ctcattcagc ttttcggtga cctcacgtac cgcatcaatg
     9961 cccagcatat ttttcccttt ttcgggagcc agggtgtagt aatcgggatg cgtgccagcc
    10021 tgcatcaact gacatccacg acagtgaccg caacttttgt ggccctgcgg ttgttggcag
    10081 agtaaataac ggctcagggc gtagattaaa gcatcatcgc ccatgcccgg taacgcctga
    10141 atgagtagcg catggtgacc tcttccggcc tgatagctgg ctaccagttt ttcgaaatca
    10201 ggtcgtaacc atggatacca tctcatgcgt ccaactcctt aacccagtgg gtcacggtag
    10261 tgcggattgc atccatcacg gcctccagcg gctgggtggc atcaatggta tgaatacttt
    10321 tatcttgtgc agccagttcc agatagcggg cgcgggtgcg attaaagaaa tcgaaagact
    10381 cttgctcaat acgatccagc tcgccgcgcg cacgcgcgcg ttttaagcca acttccgggg
    10441 taacatcgag atagagcgtt aagtcggggc gaaaatcccc gagaacagca tcacgcagtg
    10501 ttgccagcat atgttggtca ataccacgtc ctccgccctg atacgcctga gtggagagat
    10561 cgtggcgatc gccaatcacc caggtgccgt tagccagagc tggtttgatg accgtttcta
    10621 ccagttgaac gcgcgcggca taaaacatca gaacttcggc tttatcggta atgacttcat
    10681 cgcctaccga tttgatatcc agcaccaggc ttcttaactt ttcggcaagt tgcgtaccgc
    10741 caggttcccg agtgaaaacc atgtcgcgga tacccagttg ctcaagcgtc tcaaccacca
    10801 cattacgcgc ggtagttttg cctgcgcctt ccagcccctc aatgacgata tacttactgc
    10861 gcattttttt ccttaagcac tttcagataa tcctgcacag acttgttatg gctggcaaga
    10921 ttggtattaa acgtgtgacc acctttacca tcggccacaa aatagagata cggcgttttt
    10981 gccggatgcg cagcagcctt cagcgaatcc gcccccggcg tagctatcgc acccggcggc
    11041 aaaccggtaa tggtataggt gttatacgct gtcggcgttt ccaggtctgc acgagaaagt
    11101 ttgccattat aacgctctcc catcccgtaa atcacggttg ggtcggtctg caggcgcata
    11161 ccaatgcgta aacggttgat aaataccgag gcaacctgat cgcgttcact ggcaacggcg
    11221 gtttcttttt cgataattga tgccatcgtc accagctgat ttttatcttt ataaggcaga
    11281 ccgtccgcac gcccttccca ggcgctatcg accgctttca ccattttctt gtgcgctcgc
    11341 ttgagtaacg cgacatcggt ggtattggcg gtatacatcc aggtgtctgg ccagaaccaa
    11401 ccttcaatcc actccgggtt ttccagttca agtgcctgcg ctacggtggc gtacttatcg
    11461 tcactcagcg tgtgcttgat atacggagcc tcacgcaatt gcttgaggta gtcgctcaga
    11521 cgcatccctt ctaccagtcg cagcgggaac tgtgcttctt taccgctttc caacaatttc
    11581 agcatctcgc gcacggtcat ctgcggtgta aagcggtaag tcccggcttt aaagtgagaa
    11641 agatccggtt cgatacgcag cagccattga aaaacccgtg ggcgattgat gatcttatca
    11701 gcataaagct gttcaccgag cgccagacgt ccggtccctg gcttcagggt aaatatcgtc
    11761 tcttctttga taagcaattt gctgtcggca agatggcgaa ccttccagac gcccacacca
    11821 gcggcgatac ccagtaccac cagcaataac aagataatca ataacacttt tttcatgact
    11881 aattcgggcg ctcacaaagt ggggctaaat attcatataa cgttgctgac gaaaaggaga
    11941 catcgccaca ggcacgtacg ggcatcactg gcattaacgc attacaaata accatctcat
    12001 ctgcctgcaa cgcctcttcc agagaggctt gcacttcgac aagctgataa gaggattgtg
    12061 ccagcaaacg gatacagaat tgtcgcataa tgccgttaac acctgcctga tccagtcgcg
    12121 gcgtataaac tacattgccc ttccgccaga acaaattagc cgcacagcat tccgtaaccc
    12181 acccttcgct gtcaaggacc agcgcctcat cagcgtttgt ctgctcaaga tgagagcgga
    12241 tcaatacttg ctcaagccga ttaagatgtt taatacctgc aagatgagga ttgcgcccca
    12301 gccgcaccgg gcttagcgcc aacgtcatcc cttcgttacg caaacggtcg taatgtgcag
    12361 gataaaccgt aacggagaga atccgcgttg ccggtccact gttcaatgtg ctgtaccctc
    12421 gcccgccact accgcgactg atcacgactt tcagcacacc attttgctgt tctgctgcca
    12481 gcgttttcat ctcttgttca agctgaggcc agaagtcaca gaaaatcatc aaccgctgac
    12541 aagcatcctg tagtcgctgg atatgcgccg ataacaaact gactttaccg tcgataactc
    12601 tggcggtggt aaaacaacca tcaccaaact gcgttgcccg atcgcttact gccagcgatt
    12661 cctgcttata accgttaatt aagaacatac cggctcctta tcagagaatt gttagtgtgg
    12721 cagcatgttc actacggaac aagtcggaat aaaagctaag aaaaaaggcc cgcaagcgga
    12781 ccttttatat gggtggaaaa tgacaactta gatcttttta aagatcaaag aaccattagt
    12841 gccaccgaag ccgaaggagt tacacagcgt gtattccatt ccgctgacct gacgtgcttc
    12901 atgcggtacg aaatccagat cgcaaccttc atccgggtta tccaggttga tggttggcgg
    12961 aacagcctga tcgcgcagcg ccaggatgga gtagatagat tctactgcac ccgccgcacc
    13021 taacaggtga ccggtcatag atttcgtgga gcttaccaac acacggcttg cagcttcacc
    13081 gaagatggtt ttcaccgcct gcgcttcagc tttatcgcca gccggcgtag aagtaccgtg
    13141 cgcgttaacg tagccaatct gactcgcttc aatgcctgca tcacgcagag catttgccat
    13201 tgccagagct gcgcctgcgc cattttctgg cggtgacgtc atatgataag catcgctgct
    13261 cataccaaag ccgacgagtt cagcgtaaat tttcgcaccg cgttttttcg cgtgttcgta
    13321 ctcttcaagt accagcatac cggcaccatc gcccagtacg aagccatcac gctctttatc
    13381 ccacgggcgg ctcgccgctt gcgggttatc attgcgggta gataatgcac gtgccgcgcc
    13441 aaaaccacca acgcccagcg gcgtactggc tttctctgcg ccacctgcaa ccatcacgtc
    13501 agcatcgcca tacgcgataa tacgcgcagc atgaccaatg ttgtgcacgc cggaagtaca
    13561 ggcggtcgcg atagagatgc ttgggccacg caggccatac atgatagtca gatgacctgc
    13621 caccatgttc acaatcgttg acggaacgaa gaatgggctg atcttacgtg gaccaccgtt
    13681 catcagagat gtgtggtttt cttcgatcag tccgaggccg ccaatcccgg agccaattgc
    13741 ggcaccaatg cgggttgcgt tctcttccgt tatttcaagg ccagaatcct gcatggcctg
    13801 aacgccagcg acaattccat attgaatgaa ggcatccatc ttgcgctgtt ctttgcgcga
    13861 gataatgtcc tcacagttaa aatcctttac taagccagca aatttcgttg cataggcgct
    13921 agtatcgaaa tggtcgatta ggctgatgcc actctgaccg gcaagcagag ctttccaggt
    13981 agactctacg gtattgccga caggagacaa catgcccagt ccggtcacaa ctacacgacg
    14041 cttagacacg tttgtcctcc agggagggaa aaaatgattc tagtgggaca aaaagataaa
    14101 actcaggcgg tcgaacgacc gcctggagat gttcacttac gcctggtggc cgttgatgta
    14161 atcaatggca gcctgaacgg tggtgatttt ctcagcttct tcgtccggaa tctcagtatc
    14221 aaactcttct tccagagcca ttaccagctc aacggtgtca agagaatccg cgcccaggtc
    14281 ttcaacgaaa gaagcattgt tggtaacttc ttcctgctta acgcccagct gttcgccgat
    14341 aattttctta acgcgttctt cgatagtgct catactctta aatttcctat caaaactcgc
    14401 tttcgcgatg gttttcgtag tgtataaaat gttgaaaaat ttgcaactaa atcccggcag
    14461 gtcttaccac gattttacgt tattttgagg ccttaccccc caataacgca aatcattttg
    14521 cactaattat tactcataac cacgcagact gcgcgcaaca tgagcatttt gtgcaaatcg
    14581 cggtcagacc atgtacatcc cgccgttcac atgcaaagtt tcacccgtga tgtaagctgc
    14641 ttcgtcggat gccaggaatg caaccgcgtt ggcgatttcc tgtgcgccgc cgaggcgacc
    14701 cgcaggaacc tgcgccagga tacccgcacg ctggtcatcg ctcagcgcac gtgtcatgtc
    14761 cgtttcaata aagcccggag caacaacgtt tacagtaata ccgcgtgacg caacttcgcg
    14821 cgccagtgat ttactgaagc cgatcaagcc cgctttcgcc gcagcgtagt tggcctgacc
    14881 gccatttccc atggtaccaa ccacagaacc gatagtgata atacgaccat gacgcttttt
    14941 catcatagcg cgcattaccg cttttgacag acggaaaaca gatgaaaggt tggtttcgat
    15001 aatttcgttc cactcttcat ctttcattcg cattaacagg ttatcacgag tgataccggc
    15061 attattgacc aggatatcca cttcaccaaa ttctgcgcga attttttcca gaacagattc
    15121 gatagatgcc gggtcggtca cattcaacat cagacctttg ccgttggcac ctaaataatc
    15181 gctgatcgcc tgagcgccat tttcactggt cgcagtgcca ataactttcg cgccacgggc
    15241 tgcgagcgtt tcagcaattg cgcggccaat tccgcggctt gcaccggtta ccagtgcgat
    15301 ttttccttca aaattcatga ttttcctctt ttaaagctcg agcgccgctg ccatcgctga
    15361 aggttcgttc agcgccgagg cggtcagggt gtcgacaatg cgtttcgtca ggccagtaag
    15421 caccttgccc gggccgactt catagagatg ttctacgcct tgcgctgcca tgtactcaac
    15481 agacttcgtc cactgaaccg ggttatacaa ctgacgtacc agtgcgtcac ggatggcatc
    15541 accattggtt tcgcatttca catcaacgtt attcacaaca gaaactgttg gtgcgttaaa
    15601 ggtgattttc gctaattcta ctgccagttt gtcggctgct ggtttcatca gcgcacagtg
    15661 agacggtacg ctcactggta acggcagcgc gcgttttgcg ccagcagctt tacaggcagc
    15721 gccagcacgc tcaaccgctt ctttatgacc ggcaataacc acctgtcccg gagagttaaa
    15781 gtttaccgga gaaacgacct gaccttctgc agcttcttca cacgctttcg caatagacgc
    15841 atcatccaga ccgatgattg ccgccatagc gccagtgcct tccggtacgg cttcttgcat
    15901 gaacttaccg cgcatctcaa ccagacgcac cgcatcagcg aaattaatca cgccagcgca
    15961 aaccagggcg gagtattccc ccaggctgtg accggccatc attgccggtg ctttaccgcc
    16021 ctgctgctgc catacgcgat acagcgcaac agatgcaatc aacagcgctg gctgggtttg
    16081 ccaggtttta ttcagttctt cagctggccc ctgctgggtc agcgcccaca ggtcgtagcc
    16141 cagcgccgca gaagcttcag caaacgtttc ttcgacaatt ggatagctcg ccgccatatc
    16201 agccagcatt ccaacggttt gagaaccctg tccagggaac acaaatgcaa attgcgtcat
    16261 gttttaatcc ttatcctaga aacgaaccag cgcggagccc caggtgaatc caccgccaaa
    16321 ggcttcaagc agaaccaact gccccggctt aatgcgcccg tcgcgtacag cttcatccag
    16381 cgcgcacggg acagaggccg cagaggtatt accgtggcga tccagcgtca ccacgacatt
    16441 gtccatagac ataccgagtt ttttcgccgt tgcactgata atacgcaggt tagcctgatg
    16501 cggaaccagc cagtccagtt gagaacggtc aagattattt gccgccagcg tctcatcaac
    16561 gatgtgcgcc agttccgtta ccgcaacctt gaagacttcg ttgcccgcca tcgtcagatg
    16621 aattgaattc tctggattca cacggtcagc attaggcagc gtcagcaact caccatagct
    16681 accgtcggca tgcagatggg tggagatgat tcccggctct tcagaggcag ccagcaccgc
    16741 agcgcccgcg ccatcgccaa aaataataat agtcccacga tcggttggat cgcaggtgcg
    16801 cgccagtaca tcggaaccga cgaccagagc atacttcacc gccccagatt tcacgtattg
    16861 atcggctacg cttaatgcat aggtgaaacc tgcgcaggct gctgcaacgt caaatgccgg
    16921 gcaaccttta atgcccaaca tgctttgaat ctgacaagct gcgctcggga aagcgtgcgt
    16981 agcagaagtc gttgccacaa cgatcaggcc aatctggtct ttctcaatgc ccgccatctc
    17041 aattgcgcgt gtcgccgctt caaagcccat ggttgaaacg gtttcgtttg gcgcggcaat
    17101 gtggcgttcg cggataccgg tacgagtgac aatccactcg tcagaggtgt ccaccatttt
    17161 ttccaaatcg gcgtttgtcc gcacttgttc gggcagatag ctgccagtac caataatctt
    17221 cgtatacatg tacgctcagt cacttttcgg ttatataccg tcacttgcaa actgcgagtt
    17281 cgctggcagc gtcctgctac cgcagagttc cgcttttgcc accgtccagc agctcaaaac
    17341 cagctgggta tacagattcc aggcgagcgg caattcgctg aggaacttgt cgctgcaccg
    17401 cctgcactgc ctgttcaatc gcgaccgcaa aagctcgctg attggctgca ccatgacttt
    17461 ttatcaccgt gccgcgcaat cctaacagac aggcgccgtt atactggtcg gggttgaggt
    17521 gactgaatcg cctcgtcagg ctcttttgta gccaacgctt taataacagt agccaccacg
    17581 accgtttttt cccttcaccc tgagatttca gcagagaaag gaacatcctg acaacacctt
    17641 ccatcgtctt taacgtgaca tttcctgtaa agccgtcaca aaccagcaca tctgtcttgc
    17701 cagttaacaa ctcattggct tcaagatagc cgatataatt gatagaaggg attgttttaa
    17761 gcaccgctga ggcatcccga atactgtcga gaccctttac ttcttcttca ccaatattga
    17821 gcaacgccac gcgaggattg ggaatttcca ccacctcttc agccagaact gagcccataa
    17881 tggcaaattg caccaacatt gtgctgtcac aatcgacgtt ggcccctaaa tcgaggacca
    17941 ccgttttgcc cttttgctga tgtggtaata ccgtcaccag cgccggacgc tcaatcccct
    18001 ccaggggctt gagtaataat tttgccagcc ccatcagtgc cccggtatta ccggcactga
    18061 cacaggcttg cgctcgacct tctttcacca gctccagggc catacgcatt gaactcccac
    18121 gactggcgcg gatagcttgc gaaggccggg catcactggc gataactgac tgcgcaggaa
    18181 taatctgcag acgcgaacgt tgttcaaagt cagctttagc aagtaatggc gtgatggcgt
    18241 cgggattgcc gactaaaaga agagtgagtt gcgaattaga attcagtgcc tgcaatgctg
    18301 caggcactgt cacggaaggg ccaaaatccc ctcccatgac atctaacgcc agggttagac
    18361 gtgtcaaggt atcgtcgccg cccggtttgg tctttcccca gttgcctggg gaaaatcctc
    18421 actaagcttc atcacgcaga cgcgtgatta cttagcgatg accttgcggc cgcggtagta
    18481 accgtcggca gtgatgtggt gacgcaggtg tttttcacca gaagttttgt ctacagacag
    18541 gctggtgact gcggtcagcg cgtcatggga acgacgcatg ccacgtttgg aacgggttgg
    18601 tttattctgt tgtacggcca tggaccttac tcctcaatta cggtttatcc ccgatccaac
    18661 ggggagcacc aattacttac gctttaagct ggctaatacg gcaaatgggt ttggcttttg
    18721 cgcttcttca ggcagttcac caaagaccat gtccgcttcg gacacttcac agtgttcaga
    18781 atcatgcacc ggaactaccg gcaaggcgag gatgatttca tcttcaacca ttgcaagcag
    18841 atcgatttca ccgaattcgt taacctcaat cggttcatac gcttccggca gtgcttcagc
    18901 ctgttcgtct gaacgcacag gactaaaaca atacgttgtg tagacctgat gagtaaacgg
    18961 cttcccgcaa cgctgacact cgagcgttac cgtcaccttc gcatcgccgt ttaacactgc
    19021 gagacgttgg ttatcgatag cgaacgacat ggagcattcc acatcactgt ccacactgac
    19081 tacggattcg gcgacgcgct caacctgatc aggggtatag ataccctggt aatcaaggcg
    19141 tttttgagcc gtacgaaccg gatcgagagt caggggtaat tttacctttt gcatagggcg
    19201 cgcatattaa ctttgtaacg tcatagagtc aaagaaaaag gcagcctgtg gttgcctttt
    19261 gccaataatt cgcacacatt gcgggttact gctttatttt cgttcagtgc tgataacgaa
    19321 catcaacctg caaaacgaag cgcatagttt aaaatggcta tcatcaatac gctatatctg
    19381 atggaaaaaa atatgcctaa acttatttta gcctccacct cgccatggcg tcgcgctctt
    19441 ctggaaaaac tgcaaatctc ttttgaatgt gcagcaccag aggtcgacga aaccccacgc
    19501 agcgacgaat caccgcgaca gttggtgctt cgactggcac aagaaaaagc gcaatctctg
    19561 gcgtcacgtt atccggatca tttaattatt ggttcagacc aggtatgtgt tcttgatggc
    19621 gaaatcaccg gtaaaccgtt aacggaagaa aatgcccgtc tgcaattacg caaagccagc
    19681 ggcaatatcg tcaccttcta tactggactg gcgttgttta attcggcgaa tgggcatcta
    19741 caaacagaag tggagccttt tgacgtccat ttccgtcacc tgagcgaggc ggagattgat
    19801 aattacgtgc gtaaggagca tcccctgcac tgcgcgggta gctttaagag tgaaggattt
    19861 ggcattacgc tgtttgagcg tttagaggga cgtgacccta acacgctggt tggtttaccg
    19921 ctaatcgcgc tgtgtcagat gttacgtcgg gaagggaaaa acccgctgat gggatagttg
    19981 tttgcgccgg atgcggttac gtcgcatccg gcaatcggtg caggcggcct gatgcggcgt
    20041 aaacgcctta tcaggccaac cgcccattat caggcttata tgcttgtatt agcgcgcgtt
    20101 acgcagcttt tgcaaacaac gcttcaaacc ttcatccatc ggcgcttcga tacgcatcac
    20161 ctcaccggtc cccggatggg taaacttcaa cgctgcggcg tgcaggaaca gacgatttaa
    20221 tcccgtgcct gcttcagtga gctgtctgtc aaattcacgg tcaccgtagc gatcgtcaaa
    20281 ggcaatcgga tgacccgcat actgtgtatg cacacggatc tgatgagtac gtccggttac
    20341 cgggctacaa cgcaccaggg tggcaaatgc atagcgttct tccactttaa agcgtgtttc
    20401 cgacggtttg ccttcctgac tgacacgcac gatacgttcg ccgctttgca gaatattttt
    20461 caataacggc gcttgaacgc tcttcacatg cgactgccac tgaccgcgca ccagcgccag
    20521 gtaatctttt tgcattcctt tttcacgtaa ttgctcatgc agagaacgca acgccgagcg
    20581 ttttttcgct accagcaaca cacctgaagt gtcccggtcg agacgatgaa ccagttcaag
    20641 gaaccgcgct tccgggcgca acgcccgcaa accttcgata acgccgaagc ttaaaccact
    20701 gccgccatgt accgccgtac cggaaggttt attcagcacc aggatatgat catcttcata
    20761 taagatgacg tccgccagcg ccgccacctt ttgcagatgt ggcgaaaccg cctcttcttc
    20821 ccgctcagca acgcgaaccg gtgggatacg cacctcatca cccgcttcga gtttatattc
    20881 aggcttaata cgttttttgt tcacccgcac ttcaccttta cgcaaaatac ggtaaatcat
    20941 actttttggt acgcctttca attgggtacg caaaaagtta tcgatacgtt gccccgcttc
    21001 gtcagcggtg atagcaacaa tttttacgga tggagtctct gttttcatgg tgggcgattc
    21061 taattagcca acaggattcg cgccactcat ttttctatgc ttatatttag ttttacacct
    21121 tattacttca ctacgtgatc actttattga tggttattaa accaatcacc agcaagaagt
    21181 gaaaaaactg tgagtaagcg ggtgataaat ggtaaaagtc atcttgctat aacaaggctt
    21241 gcagtggaat aatgaggccg tttccgtgtc catccttgtt aaaacaagaa attttacgga
    21301 ataacccatt ttgcccgacc gatcatccac gcagcaatgg cgtaagacgt attgatcttt
    21361 caggcagtta gcgggctgcg ggttgcagtc cttaccggta gatggaaata tttctggaga
    21421 gtaataccca gtctgtttct ttgataattg cgctgttttt ccgcatgaaa aacgggcaac
    21481 cgacactctg cgcctctttg agctgacgat aaccgtgagg ttggcgacgc gactagacac
    21541 gaggccatcg gttcacaccc ggaaaggcgt tactttgccc gcagcttagt cgtcaatgta
    21601 agaataatga gtaagttacg atgaaaagaa tgttaatcaa cgcaactcag caggaagagt
    21661 tgcgcgttgc ccttgtagat gggcagcgtc tgtatgacct ggatatcgaa agtccagggc
    21721 acgagcagaa aaaggcaaac atctacaaag gtaaaatcac ccgcattgaa ccgagtctgg
    21781 aagctgcttt tgttgattac ggcgctgaac gtcacggttt cctcccacta aaagaaattg
    21841 cccgcgaata tttccctgct aactacagtg ctcatggtcg tcccaacatt aaagatgtgt
    21901 tgcgtgaagg tcaggaagtc attgttcaga tcgataaaga agagcgcggc aacaaaggcg
    21961 cggcattaac cacctttatc agtctggcgg gtagctatct ggttctgatg ccgaacaacc
    22021 cgcgcgcggg tggcatttct cgccgtatcg aaggcgacga ccgtaccgaa ttaaaagaag
    22081 cactggcgag ccttgaactg ccggaaggca tggggcttat cgtgcgcacc gctggcgtcg
    22141 gcaaatctgc tgaggcgctg caatgggatt taagcttccg tctgaaacac tgggaagcca
    22201 tcaaaaaagc cgctgaaagc cgcccggctc cgttcctgat tcatcaggag agcaacgtaa
    22261 tcgttcgcgc attccgcgat tacttacgtc aggacatcgg cgaaatcctt atcgataacc
    22321 cgaaagtgct cgaactggca cgtcagcata tcgctgcatt aggtcgcccg gatttcagca
    22381 gcaaaatcaa actgtacacc ggcgagatcc cgctgttcag ccactaccag atcgagtcgc
    22441 agatcgaatc cgccttccag cgtgaagtcc gtctgccgtc cggtggttcc attgttatcg
    22501 acagcaccga agcgttaacc gccatcgaca tcaactccgc acgcgcgacc cgcggcggcg
    22561 atatcgaaga aactgcgttt aacaccaacc tcgaagctgc cgatgagatt gctcgtcagc
    22621 tgcgcctgcg tgacctcggc ggcctgattg ttatcgactt catcgacatg acgcctgtac
    22681 gccaccagcg tgcggtagaa aaccgtctgc gtgaagcggt gcgtcaggac cgtgcgcgta
    22741 ttcaaatcag ccatatttct cgctttggcc tgctggaaat gtcccgtcag cgcctgagcc
    22801 catcactggg tgaatccagt catcacgttt gtccgcgttg ttctggtact ggcaccgtgc
    22861 gtgacaacga atcgctgtcg ctctctattc tgcgtctgat cgaagaagaa gcgctgaaag
    22921 agaacaccca ggaagttcac gccattgttc ctgtgccaat cgcttcttac ctgctgaatg
    22981 aaaaacgttc tgcggtaaat gccattgaaa ctcgtcagga cggtgtgcgc tgtgtaattg
    23041 tgccaaacga tcagatggaa accccgcact accacgtgct gcgcgtgcgt aaaggggaag
    23101 aaaccccaac cttaagctac atgctgccga agctgcatga agaagcgatg gcgctgccgt
    23161 ctgaagaaga gttcgctgaa cgtaagcgtc cggaacaacc tgcgctggca acctttgcca
    23221 tgccggatgt gccgccagcg ccaacgccag ctgaacctgc cgcgcctgtt gtagccccag
    23281 caccgaaagc tgcaccggca acaccagcaa ctcctgcaca acctgggctg ttgagccgct
    23341 tcttcggcgc actgaaagcg ctgttcagcg gtagtgaaga aaccaaaccg accgagcaac
    23401 cagcaccgaa agcagaagcg aaaccggaac gtcaacagga tcgtcgcaag cctcgtcaga
    23461 acaaccgccg tgaccgtaat gagcgccgcg acacccgtag tgaacgtact gaaggcagcg
    23521 ataatcgcga agaaaaccgt cgtaatcgtc gccaggcaca gcagcagact gccgagacgc
    23581 gtgagagccg tcagcaagct gaggtaacgg aaaaagcacg taccaccgac gagcagcaag
    23641 cgccgcgtcg tgaacgtagc cgccgccgta acgatgataa acgtcaggcg caacaagaag
    23701 cgaaggcgct gaatgttgaa gagcaatctg ttcaggaaac cgaacaggaa gaacgtgtac
    23761 gtccggttca gccgcgtcgt aaacagcgtc agctcaatca gaaagtgcgt tacgagcaaa
    23821 gcgtagccga agaagcggta gtcgcaccgg tggttgaaga aactgccgct gccgaaccaa
    23881 ttgttcagga agctccagct ccacgcacag aactggtgaa agtcccgctg ccagtcgtag
    23941 cgcaaactgc accagaacag caagaagaga acaatgctga taaccgtgac aacggtggca
    24001 tgccgcgtcg ttctcgccgc tcgcctcgtc acctgcgcgt gagtggtcag cgtcgtcgtc
    24061 gctatcgtga cgagcgttat ccaacccagt cgccaatgcc gttgaccgta gcgtgcgcgt
    24121 ctccggaact ggcctctggc aaagtctgga tccgctatcc aattgtacgt ccgcaagatg
    24181 tacaagttga agagcagcgc gaacaggaag aagtacaagt gcagccgatg gtgactgagg
    24241 tccctgtcgc cgccgctgtc gaacctgttg ttagcgcgcc agttgttgaa gaaatggctg
    24301 aagtcgtaga agcccccgta ccggttgccg aaccgcaacc ggaagtggtt gaaacgacgc
    24361 atcctgaagt gattgccgcc gcggtaactg aacagccgca ggtgattacc gagtctgatg
    24421 ttgccgtagc ccaggaagtc gcagaacacg cagaaccggt ggttgaaccg caggaagaga
    24481 cggcagatat tgaagaagtt gccgaaactg ctgaggttgt ggttgctgaa cctgaagttg
    24541 ttgctcaacc tgccgcgcca gtcgtcgctg aagtcgcagc agaagttgaa acggtagccg
    24601 cggttgaacc agagatcacc gttgagcata accacgctac cgcgccaatg acgcgcgctc
    24661 cggcaccgga atatgttccg gaggcaccgc gtcacagtga ctggcagcgc cctacttttg
    24721 ccttcgaagg taaaggtgcc gcaggtggtc atacggcaac gcatcatgcc tctgccgctc
    24781 ctgcgcgtcc gcaacctgtt gagtaataat tagctcaaag taatcaagcc ctggtaactg
    24841 ccagggcttt tttatttcat ctttgaatca acatattaaa attcatcaaa tggcatcctt
    24901 gctaaccaac aatgcaaaat aggcaaaaaa atgcccgccc cggcgatccc agagcaggca
    24961 gacaaaacat acccagtttc atgatatgtt ttaaagcgag cttatttgtt gagctggaag
    25021 agcgacaatc cctgcatatc ggtaaatgct ttatacgatg cctgcaatgc cgtttgctgc
    25081 atgatgtaag atgaaatagt tgcattccag tccacatcaa ccagatcgct catctgctgc
    25141 gtttgcccta aagcgcgatc gctacctaat gaatccagcg actccagttc gttcagctgc
    25201 gtgcctaatt ccgcgcgaac tgtaaggaca ttattcagcg agtttttcag tccacggttg
    25261 gttttatcca atgccgcagc ggcgatttct ttatccgctt cgctatccgc gaccggcgtt
    25321 ttcagggctg cgatggcgct atccagcatg gcaaaaagat tggtttcaga agcgctaccg
    25381 tctggttccg ctaccgcgtt actggtaata ctgtcgaaaa ttttgtcgcc cgtatgtcct
    25441 atcaccatcg aacgcgaagc atcgacctgt tgcctaatac tttctgctcc accgacgtat
    25501 ttccctttct cttcgctaaa cggcgcagtc tctgttttat aaccggcaaa aatgtagcgc
    25561 ccgttaccgt cagtggtgtt cgccagattc agcaactggt cacgaagccc ctggatatcc
    25621 gtagccagcg aggcccggtc atcgtcactc aaagtgccat tgctggcgta gacaattttt
    25681 tcctgagcat tctggatagc agtggtgacc tggctaagta cactctcttc cagtgacact
    25741 ttttgagtgg cgaaagtacg cgccagcgtg tactggctgt tttgcgcctg tgcctgggag
    25801 agaacaaccg cttgtgatgc tgcaatggga tcgtcagaag ggttaacgac tcgcttacct
    25861 gtcgacatct gctcgccgta cttcatccat tccgcctgag aattggtgat gccacgcatg
    25921 ttttgctggt acatcatctg ggtactgaaa cgcatcttat ctccccttag cgaatgttaa
    25981 tcagcgcatc aaaaatcgcg tttgccgtct gcagaacctg cgcattcgcc aggtaatact
    26041 gctgaaaacg ttgcagattt ccgtactcct catcgagatt gacaccggaa atcgactgct
    26101 gctgattgga aagctgcgtc accacattac cttgcgtggc gctactggtt ttcaacgtcg
    26161 cggttttatt accgatatca ctcactaacg aggcataagc gtcgttaaag gatttcgcac
    26221 cgcccaccgt tttactgttg ctttgcagat ccagcagggc ctgaccgttg cggttatcgc
    26281 tatcacccgc atcttcttcg ctcgccatcg ctattttcgc ttcgtcggtg attaatacat
    26341 ccatgttgac gatagcgtca cttactggtt tcagcgtgaa gctgtcgtta acagcaggcg
    26401 ttcctgtaaa cgtcaactcc agaccatcaa atgccacttt accgttggca tccggcgtca
    26461 ccgtaaaagt ggtattactg gcaaggcggg tgacctgcca ctgattatta tcgaacgaga
    26521 ttttgtaatc agtcgccagt accgcggagg catcagttac cgtggcaccg atcgcaacgt
    26581 cacctgtgtt tttcgtgttt tgcagaaccg cgggcttacc gatagcaaag aaatcttcac
    26641 cagcatcgcc gttggcatca aatccggctt tgtgttggct gttgaaagcc tcggcaaatg
    26701 ccagcgccag ttgtccaagc gtattacgcg tctggtccag atcctgagaa cggaatgtca
    26761 gaatgccgcc cagcgacccg gtattcagta atttctccgg gatctcaata ttgcctgccg
    26821 tcccatcaac gtaagcgaca gtcgtacgag aagggtcggc gctggaagga actgccgcca
    26881 gttgccgcgc cgtacttccc tgaaccagtg agtaaccatt ggccatcgtg atgttataag
    26941 taccgccatc ctgaacgctg acttctacgc caacaatctg gtttaattcg ctcaccagtt
    27001 gatcgcgttg atccagcaga ttgttaggtg acgcccctgc ccccacgcct gtcaggcgcg
    27061 agatttgatc gttcaggctg gcaatttgtt tagcgtaatt gttgatctga tcaacgctgg
    27121 cacctatcgc gatattgacc tgtttgtcct ggtcgcgcag atattgatcg gtggttttaa
    27181 actgattcac caacccttct gatttcccaa tcagcgcctg gcgcgctgcc gggtcttccg
    27241 cgttactcac cagcgtttgc aggctggtga agaaatcctg catctgtgtt gccagcgaag
    27301 aggtactggt ggagagcata ttgtcgattt tcgacatctg ctcatagcgg gcagtcaggc
    27361 cgctactttg cgtctgcgcc gcacgtaact ggttggtaat aaaagcatca tactcacgct
    27421 gcacaccaga aacgtagaca ccattgccaa cccagccgcc agcgcccaac gtgctattgg
    27481 cctgcgccat aatagtggtt tggcgggtat atccggcaac gttatagctg gagatattat
    27541 tacttgccgt atttaacgcc gcctgggccg cgttcagtcc gctcatggcg ttattgatca
    27601 agctggacat ggaggttcct tataagcctt caattacaga gtattatcgg cagcgacccg
    27661 ccggacttga gttattcaga acagattatc aatgttcatg ctgtaggttt tgctcacctt
    27721 gtcgcttatc gatttcatct gctggatcat gttggtgagt ttacgggcat agtgaggatc
    27781 ggtagcatac cccgcgtcct gtagggcctg cgccccctgc tccgcactcg cggcggtcgt
    27841 cacggcggcg tagcgcgggt tacgtgttaa cagcccaacg taatccgaca atgcttccag
    27901 atacgagctg tagacccgaa acttcgcttt tactttcttc gcttcgccgt tttcatattc
    27961 agtcgtggtg atttcagtaa ctggcccttt ccagttgcca gaggctttga caccaaacag
    28021 gttatagctc ggctcgccgt tttcgcggcg gatttgccgt tgcccccaac cagattccag
    28081 tgccgcctga gcgaggatca aatgatgtgg cacaccgctt tgctggcttg ccagttgggc
    28141 gggcagcgag agttgcgcga ggaatgcttt actgtcaccc ggcagcgaat catcgtagtt
    28201 acgtggcacg gccttttgca ccagctgcga aagcgcctga ttttgataac gcaccacagt
    28261 ttcgagcggg aatttcatcg gtgctgctgg cgtggactcc tctggcaatg gttgttctgg
    28321 cgtcatctgt ttaaccatca tctctgcaag ccccagacct ttgcccgccg tcatctgttg
    28381 ggcaatctgc tggtcataca tactggtata caggcgagtg tgctcgctgc tgaacaggcc
    28441 atcttttggt aaagcgtcgc gcatgctttt caacatcatc tgcacgaaca tcccttccac
    28501 ctgacgggcc accggacgga tatttgccgc cggatcttcg cccgctttcg cctttagttc
    28561 gttgagtgat tgcgcatccc aggccgcact tgccagtagt ttgctgtcgc tgatcatcag
    28621 atgatttcca gttttgcccg cagacatccc gcactttgca ttgattgcag tatggacatc
    28681 agatccatcg gcgtagcgcc cagcgcattg agcgcgcgca ccacgttatt gaggctggcg
    28741 ctggaacgta cgctttgcag cgaaccgccg ctctggcgta aatcgatctg cgtttgtgga
    28801 gtaaccacag tctgtccacc accaaacggt gtatctggct ggctgacatt ggcctgacga
    28861 ttgactgtta ctgagagatt tccctgcgct accgcgcagc tgtcgagggt cacttcgcga
    28921 ttcatcacca ccgaaccggt gcgcgagtta atcactactt tagcgtcctg cggggtgaca
    28981 ttaacctgca tattctggat atctgcaagg aaacggacct gggaactgtt gccacttggt
    29041 acgcgcacct gaatagtccg cgcatctaac gcggtggcgc tgccatatcc acgcacgcgg
    29101 ttgatggtgt cagcgatttg ctgcgccatg ctgaaatctt cgtcgttaag ttgcaaatta
    29161 agggtattcc cgacgccaaa ctggctgggc aattcacgtt caataaccgc accattggtg
    29221 atccgtccac cgttcagttg gttcacctga acactgctac cgccagcgga ggctcctgcg
    29281 ccgccaacca gaatattgcc ctgcgccagc gcatacacct gactgtcaac gcccttaagc
    29341 ggcgtcatca acaacgtacc tccacgcaag cttttggcat ttcccatgga agaaaccacc
    29401 acatcgatgg tttgcccctg acgtccaaac ggaggaagtg acgctgtcac cattaccgca
    29461 gcgacgtttt ttagctgcat attggtgccc gtcggaacgg taattcccag ctgtgagagc
    29521 atgttattaa gcgtttgtgt ggtaaacggc gtctgggttg tctggtcacc ggtgccatcc
    29581 agccccacca ccagaccata gccaatcagt gagttttgcc ttaccccctg aacactggtg
    29641 agatcgcgaa tacgctcagc ctgagccgcc gtcgtaacca gtagaagaat taatgcagag
    29701 agaaatttaa tcaccacagc ctcacttaca ttggcgacag gttaaggaag aaacgctgca
    29761 accagcccat attttgcgct tcgttaatgt agccattgcc tacgtattca atgcgcgcat
    29821 ccgccacctg agtagacggt acggtattgc tgccgctgat agtgcgtgga ttaaccacgc
    29881 cagagaagcg aataaattcg gtaccctgat taatggcaat ctgtttttca cccaccacat
    29941 gcaggttgcc gttgaccagt acctggtcaa ccgtcaccgt caacgtgccg ctaaaggtat
    30001 tgctggcatt ggccccgccc tttccgttga acgtgttacc accggaggct tcgacatcgg
    30061 cacgagcgtt accaaacagc ccctgcaaat agcgcggcac agtatcaaag ccaaaattag
    30121 ttttaccgtc acggctggca ttcgcagagg agcttttgct ggcgctgacg ttctcctgca
    30181 acacgatggt cagcgtatcg ccaatattgc gtggtcgacg atcttcaaac agcggttgat
    30241 agccatagtt aatcggctga gcagactgga aaatagaacc gttggcgacg ggcgtcggcc
    30301 cgggaaccgg ttgtgcactg gtcgccccct gcaccagcgg cgtggagggt atccaggcgc
    30361 agccggttag tgaaagcacc aacaagctgg aaatggcata agtatgcgca gcgttttttt
    30421 gcatggctat catcttcaaa aatcagtaaa ccggtgaacc tgccaccggt taagccttag
    30481 agttgcgtca gtttttgcag catctgatcg gtggtggaca ccgctttact gttgatttcg
    30541 taagcgcgtt gcacctgaat catattgacc agttcttccg ccacgttgac gttagacgtt
    30601 tcaacatacc cttgatacag cagtcccgcg ccgttcaggc ccggcgtgct ttcgttcggt
    30661 gcaccagagg attgcgtttc ggtgtagagg ttttcgccaa tgctctccag cccggtgtca
    30721 ttcataaagg tggtgagatt gagctgccca acctgaaccg gagctgcctg gccttgttgg
    30781 gttacgctga ccacgccatc acgaccgatg gtgatactta acgcattcgc cggaatggtg
    30841 atcgctggct gcacctgaaa accaccagcc gtcaccagct gcccgttctg atccacctgg
    30901 aaagagccgt cacgggtata ggctgatgaa ccatctggca acatcacctg gaaaaagccc
    30961 tgccctttaa tcgcgacatc tttgctgttg ttggtctgcg acaggtttcc ctggctgtgt
    31021 aagcgttcag tggcgaccgg gcgtaccccc gtgccgattt gtaatccgga gggtaaggtg
    31081 gtttgttcgg aagactgtgc ccccggctgg cgaatggttt gataaagcag atcttcaaac
    31141 accgcgcgct gacgcttaaa accgttagta ctgacgtttg ccaggttgtt ggcaatgacg
    31201 tccatattgg tttgctgggc gtcaaggccc gttttggcga tccataatga actgatcata
    31261 aaatgtcctg tagtggatta gtgacaagca gagcagaagc acgcgacagt gtgaatttcc
    31321 cctcacccta accctctccc caaaggggcg agggaatagt ccgcgcgctt ttattgcaat
    31381 gtcatcaatc gttcaattaa ctcatcgaca gcagttggtt ggcacggcct gcgttatcat
    31441 cgacgctgct gatcaccttc atctgcattt caaaacgccg cgcgctggca atcatgtcgc
    31501 tcattgccgc aacggcattg acgttactgc cttccagaac ccccgacatc acacgcaagg
    31561 ttggatctgc ctgcagtatc ggcccacgcg tggcctggct ttctgcgctt aaacgaaaaa
    31621 tgccgtcgtc accgcgttgc acttcgctgc ccgtggcttt caccagtttc agacgcccca
    31681 ctggcgcaac cgtatttgcc ggatcgcccg gattgagcgc cgagattgtg ccatcggcag
    31741 caatagtgat ttccgcccct tccggcacag caattggccc agcctcgcct atcaccggat
    31801 gcccctgaat tgtcagttgc ccggtgggat caacctgaat gctgccatta cgcgtatacc
    31861 cttcgctgcc gtcagcggac tgcacggcca gccagccatc ctgctgcaat gcaacgtcca
    31921 gcgggcgcga ggtgtaatcc atcttgccgg gcgtcatgtc tgcacccggt gttgacgccg
    31981 ttaccagcgt gcgcgtgggc agtgaaagcc cttccaccgg cactgcacgc aaggcgttta
    32041 actgcgcgcg aaaaccgggc gtagaggcat tggccagatt actggcggtt accgcctgtt
    32101 gattcagcgt ctggctggct gctcccatcg cggtataaat tgcgtgatcc attgagctat
    32161 cccgtcagcg attagcgtaa gttaaccagc gtgttgagga tctggtcctg ggttttgatg
    32221 gtctgggcgt tagactgata gttacgctgg gcaacgatca tattgaccag ttctttactg
    32281 agatcgacgt tggacgcttc caacgcaccg ttggtcaggg tgccaaagtt gcccgtcccg
    32341 gctgtcccca acagcgccac gccagaagat tgcgtcgcag accagacgtt gtcgccttcg
    32401 gatgccagac cttcgttgtt ggcaaagttc gccagtacaa tctgccccag cagttgtgtt
    32461 tgttcgttgg aatagttgcc gacaaccgta ccgtcatcat tgatttgata actcaccaga
    32521 tcgcccggtt tgtagccatt ctgggtggtt gccacaatat tgttagcgcc ggtattttgc
    32581 tgcatggagt tgaggaagct caggctaaac gtggcgggat ctgcgccgtt aattgcgcca
    32641 gtggtaatat tctctgttgg atttgaggtc agaacgccat tggcattaaa caccagcttc
    32701 attgcaggct cggctgtacc tgttggatca ctgctatcct gggtgtagac ctgccagtta
    32761 ttatccccgg tcttcacaaa gtagacgctc atgtcatgag cattaccctg actgtcgaaa
    32821 acagtcaccg aacctttttt gttatagcta tccgcattgc tggcgctgaa tggcgtaaca
    32881 gtaggaagcg gatcactgga attcaggttg atctgcatcg atgccgtggt ggtagttttc
    32941 gctgccatca gggtattcgg gatcgaaata ttggtcggat tcgccccttg ctgaatagtc
    33001 ggcggcgtac cggttgccgg gtaacccgtc agctgtaaac cttgcatatt caccaggtta
    33061 cggttttcat ccagcttaaa ttgtccgtta cggctgtaga acaccgaacc gttgctgtct
    33121 accagacgga aaaaaccgtt ctggctgata gcaacgtcca gacctcgccc ggtgttggtg
    33181 gtcgtgccat cggtaaagtc ctgagtgata ccggcaactt ttacccccag tcccactttc
    33241 gaaccggcaa acatatcggc aaaagaggcc gtgcctgatt taaagccgta ggtggcggag
    33301 ttggcgatat tgttgccaat aacatcgagg ttggtggcgg cagcgtttaa tccgctaacc
    33361 gcttgagaaa aggccatgac tgactcctga aatgtgaagg cttaaattat ctgccgtact
    33421 tcgtcgaggg tggtggtgcc gtaagtgccg agatccagcg tattaccgct gttgccgcgg
    33481 atcacaccct gcaccagagc aaactgcagc ggctgggcaa ccagttgtgt accaccgtta
    33541 ctggcgctaa tcgctacatt gtaagaaccg ttcggcgcag tgctgccgtc agtcaacgta
    33601 ccgtcccagg tgaaactgtg aactccagcg gtcagttcac caatatcaat ggtgcgcaca
    33661 accgcaccat ttttatcggt gatggtggcc gtaactttgt ctgccgcctg ttgcagctca
    33721 acaccaaacg gcgtggtcgt ggtcacagct ccttcttcac tgccggttcc cgcaagaaca
    33781 gtggtgccgg ggatcatcac gccgtgaccg atcaggttac tggcctgtaa cgactggctg
    33841 ttatcaatct gtccggaaat agatccgagt gtggtattga gtttttcaat cccgctgacc
    33901 gtgctgattt gtgccaattg cgacgtcagc tcgttgtttt ccattggatt ggtcgggtcc
    33961 tggtttttca gctgcgccac cagcaaagtc agaaaactgc tttgtaaatc tgcggcgttg
    34021 ctgcccgtga gcgaactact gctggtggta ctgacgccgg tatttgtcgg atcggtggtg
    34081 gttaccgcaa tggacatagc tttctccttt attgaccgag cgtaagggtt ttcagcatca
    34141 tgcttttcac cgtgttgagc acttcaacgt tggcctgata gctgcgtgac gccgacatgg
    34201 tgttaaccat ctccccgacg acatcaacgt tcggcatttt tacgtagccc tttgcatctg
    34261 ccagcggatt acccggttca taaaccagtt tgtccggggc ctgactttct ataacatcgg
    34321 caacctttac gccgcctgtc gcagcacctg gtgcagcgtt aacctggaat accacctgtt
    34381 ttgcccgata tggctgtcca tcaggaccgg tcacgctatc agcatttgcc agattactgg
    34441 ccgccacgtt caggcgctgg gactgggcag ttaacgccga cccggcgata tcaaaaatat
    34501 tcagcagtgc catccgttaa tttccgctct gtaaaacgtt catcatgcct ttgatttgcc
    34561 cgctcaacgc gctaaggctc atctggtatt gcaggctgtt atcggcaaac tgggtgcgtt
    34621 cgcgatccat atcgacggta ttaccgtcaa gcgaaggctg gtccggaata cggtattgca
    34681 gttctgcggt aggaggcgtc agcgcctgcg ccgggatgtg ttgcgttgag gtcatcgtca
    34741 gtgcaaccac actggttgca tcccgtccac gttgcatgac ttttttaagt tcactggcaa
    34801 aatcgatatc gcgcgcctga taaccagggg tatcggcatt ggcgatgttt gctgccagca
    34861 cttcctgacg ctgggcgcgc agattgagcg cctcttgttg aaaacgtaag gcggcgtcga
    34921 gcttatcgag catatctcct ccgcaggtat caaaattctg ccatcagctt aaatgccttt
    34981 acgcgcgcgt tatcggcgga ataaacgcaa aatgggtcgc tatttatgcc gttgatggtc
    35041 attgcggaca ggtacaattc acgttgtaga aatggctggg ggcgaaaatg ctgacaataa
    35101 aacgtagcgt ggcgatcatc gcgatactgt tcagtccgtt aagtacggcg agcaatctca
    35161 catcgcaatt gcacaacttt tttagcgccc aactcgcggg ggtaagtgat gaggttcgtg
    35221 tttctattcg tacagcgccc aatctactac cgccatgcga gcagccattg ctttcgatga
    35281 gcaataattc ccgcctgtgg ggcaatgtga atgtgttggc acgctgcggt aacgacaaac
    35341 gatatttaca ggttaatgtg caggccacag gaaattatgt ggttgccgcg atgcccattg
    35401 tgcggggagg aaagctggaa gctggcaatg tcaaactaaa acgcggacgg ctggataccc
    35461 tgccaccgcg tacggtgctg gatatcaatc aacttgttga tgccattagc ctgcgcgatc
    35521 tatcacccga tcaacctatc cagttaaccc agtttcgcca ggcatggcgg gtaaaagcgg
    35581 gacaacgcgt caatgtgatc gccagcggtg atgggtttag cgccaacgca gaaggtcagg
    35641 cgctgaacaa tgcagccgtc gcacagaatg cgcgggtgcg catggtatcg ggacaggtag
    35701 tcagcggcgt tgttgatgca gatgggaata ttcttataaa cctgtaagct gttaaagatt
    35761 acccgtccct tgccgataaa taagcaacac atgataaaag cgccctcaat gaggaataaa
    35821 ccatgagtat tgatcgcact tcgcctctga agcctgtaag caccgttcaa ccgcgcgaaa
    35881 ccactgacgc gccggtaacg aacagccggg cggcaaaaac aaccgcctcc accagcacca
    35941 gtgtgacgtt aagcgacgcg caagcaaaac tgatgcaacc cggcagcagt gatatcaatc
    36001 ttgaacgtgt cgaagcgtta aaactggcga ttcgtaacgg tgaactaaaa atggacaccg
    36061 gcaaaattgc cgatgcgctg atcaacgaag cgcagcaaga cttgcagagt aactgatcgt
    36121 atgacacgtc ttgcagagat cctcgaccag atgtccgctg tgcttaacga tctcaaaacg
    36181 gtaatggatc aagagcagca acatctctct atggggcaga tcaacggcag ccagttgcaa
    36241 tggattacag aacaaaaaag ctcactgctg gcgacgctgg attacctcga acagttacgc
    36301 aagaaagaac ccaatacagc aaatagcgtt gatattagtc aacgctggca ggaaattact
    36361 gtgaaaacgc agcaactacg ccaaatgaat caacataacg gctggttact ggaaggacag
    36421 attgagcgca atcaacaggc gctggaaatg ttgaaaccgc atcaggaacc gacgctatat
    36481 ggggcgaacg gtcagacctc aacaacccat cgcggcggta aaaagatttc gatctgaata
    36541 ttatctccgg cctgcactgc aggccggaat gcattgttac accgtccggc gggcaaattc
    36601 tttaactttg aagcccagta ccgccagtgc agcgaagtac gcggcaatcc ccgccagcac
    36661 gaccgccatt aaacgcagta agcgccaggg catggtaccc aatgaccact ccggcatgat
    36721 atgtaacata cctaaaagca cgccagacat caccagtacc gccatcacca gacgcaacag
    36781 aaacgccatc cagccgggtt gcggagtaaa gattttctgc ttacgcaact gccagtaaag
    36841 cagcgaagca ttcagacacg ccgccagacc aatagaaagt gacagcccgg catgtttcaa
    36901 cggaccaata aacgccaggt tcatcaattg cgttaaaatc agcgtaacga tggcaatttt
    36961 cactggcgtt ttaatgtcct ggcgggaata aaagccagga gccaacactt tcactacaat
    37021 caggccaatc aaacccaccg agtaggcaat taacgcccgc tgggtcatca gcgcatcaaa
    37081 cgcggtaaat ttaccgtact ggaacagcga aacggttaac ggaccagaaa gaatgcccaa
    37141 cgcaaccgca ctcggcagcg ccaacaggaa acaaagacgc aacccccagt ccatcaaacg
    37201 gttgtattca tcatgattgc cactggcaaa acttttcgac agcgacggca gcaaaatggt
    37261 gccaagcgcc acgcccagca caccggacgg aaactccatt aagcggtcgg cgtaatacat
    37321 ccaagacacc gaaccggaag caagaaacga ggcaaaaatg gtgttgatga ttaaggagat
    37381 ctggctcaca gagacgccaa ggatcgccgg tcccatctgt ttcaccacgc gcattgttcc
    37441 ggcatcgtgg aagttaatgc gcggcaggac cagcatgccg atcttcttca ggtgcggtag
    37501 ctgatacacc agctgcagga tgccgccgac cgtaacagcc cacgccagcg ccagcaccgg
    37561 tgggttaaag tacggtgcgg caaacagcgc gaaaccaatc atgctgatgt taagcagtgt
    37621 tggagcaaac gccggaatcg agaagcggtt ccacgtattc agaatcgctc ccaccagcga
    37681 cgccagggag atcagcaaga tatagggaaa ggtaatcttt agtagctggc tggtcagggc
    37741 aaatttgtca gctgtgtcag cgaagcctgg cgcggtcacc atgatcaccc acggtgcggc
    37801 gagcatgcca gcgaccgtca caaccgccag cgcgagtgtc agcaggccag aaacataaga
    37861 gacaaagacc cgcgtggcgt cttcaccctg cttactttta tattccgcca gaatcggtac
    37921 aaatgcctgg gaaaatgccc cttcggcaaa gatacggcgt aacaagttag gaagtttaaa
    37981 agcgacgaaa aaggcgtcgg ttgccatccc tgcgccaaag attctggcga caattgcgtc
    38041 tcgtgcgaag ccaagcacac gcgaaaacat ggtcatcgag ctgacggcgg ccagcgattt
    38101 taataaattc atcggtgttc taatccagac ccacgggcaa aacgcctgca aggcaggcgc
    38161 tgaaagaggc gaatagtcta cctggattat ggtgaattgc taccgccaga tgttacaggg
    38221 tttattcact catcgcatcg cgccagatct tgtcaacgat acgttgcgcc agcacggcct
    38281 gttcgccggc ggtttgcgga actgtctgat tttgcacaca ttcaatgaag tgccgcgcac
    38341 agccgacaaa tccacgttgt tcaagagtgc tctgccaacc aggaatcggt ttatgcacta
    38401 cgccctgccc gcgctcctca cgccattcgc gcatatccgt aatgtcgatg agcgcaccgt
    38461 cagtcacggc ctgcacggtt tcacgctgac ttccggcacg gcgatgcata caggtggtga
    38521 tctgcaaagg accggccgaa aaatggtgct cggcaaacag catttcgcca gcgtcgttag
    38581 tcagtagcgt accgccatcc agagaggctt tgccgcccga caaccacagc acggtatcca
    38641 ccacatgcag ataatcatcc agcaacgtga aataaagatc gtgtggcccg acactattgc
    38701 tacgatgttt atccattctt agcgaagctg cggtagcgag ttgcgttttt aactcaccgt
    38761 agagtggtgc gaaacgacgg ttaaaaccga ccatcaacgt cagtttttta cgcgccgcca
    38821 gttccaccag ccgttcagca tcgcgcagat tttctgccag cggtttatcg acacagacat
    38881 gtacacccgc attgagtaac gtactgacca cgtcaaagtg gctggcggtg ctggaatgca
    38941 caaaaaccgc atcgcaactg gcggcaaggc tggataacga atcggcataa ggaatgcgcc
    39001 agctttcaca aattggcagg gctttcgcgc gcgtaggcga ccaggctcct tgtaacgtcc
    39061 agtcagacgc tgccgccagc accggtaacc acgctttttg cgcaatgcca cctaatccca
    39121 ctacgccgat acgtaatttt ttcactttaa tctcccagat gggccagcaa cgaatcaaga
    39181 cgctgtttca gttctgccac ttcgatttcc agggcttcga cgcgggcctg taaatcacca
    39241 tcaaccgcgt tcgacatatc cgtcaccgcc ggctgatctt caacctcacc actgaaaaga
    39301 tgcatgtagc ggctttcgcg tttacccggt tcgcgggcca gacgcaccac aaaaggacca
    39361 tcttcgcgat ttgccagttg ttccagcgtc gactccactt ccgccatatc gctgaattca
    39421 tacattcgcg cggcgcggct gcgcagttca cctggcgtct gggcaccacg caataacaac
    39481 gtggtgatta acgccacttc cgctgcgctc agtttcagat cgccaaattc tgaattacaa
    39541 aaacgttgct catatttggt gacccgatta ccaaaaccgc tcactgtgcg tagataatga
    39601 cgtttgacca gattatccag ctgttcctgc acttcggatt cactcagatt catgaccggt
    39661 tcacggttcg ttttctgatt acaggccgtg actacaccat tgactgagag cgggtattgc
    39721 tccggcgtgg tcacctgctt ttccagcaag caaccaatca cgcgagcttc aagggcagta
    39781 agttgatatt tcatgagatt ctccttagcg gccgggcgtc cagtctgggg tagttaatgc
    39841 cgtcagtacg tgatcgcgcc attgtccatc aatcaacaga tagtctttcg catagccttc
    39901 tttttcaaaa cccagtcgcg ccagtaaatc accgctgcgt ttattgtgcg gcatataatt
    39961 agccataatg cgatgaatat gttgggtgcg ctgcatataa cgaatggctg cggtcagggc
    40021 ttcaaacatg agtcctttgc cctgccattt ttgcccaatc gaataaccga gatagcaggc
    40081 atgaaaagag ccacgaacaa cattggaaaa attggcaacg ccaataatct ctttttcgtc
    40141 cgggtcgaat aagccaaagt agaaagctga accttgttta tgaaattcgt taatcatccc
    40201 cagcctggcc tgccagcctg atggataaca gtggctttcg tcgcgcactg gctcccaggg
    40261 cttgaggaaa tggcgattct ctgcgtaata atccgcaaga cgccaggcat cacgatcatg
    40321 caccagacgc acgaccagtc ggtctgtggt taagcgcact tttggcacgt tactgcgata
    40381 gccaaacatt ctatacctac tccttcccgt aacgtcttta atacgctgta agtattacta
    40441 tacctgcgca accagaccct gtgaaaagtc caaaatgcca ttttctgcat tattcacata
    40501 tttgataaaa actctctatc aaagcgacgt tttgataaaa aaatattgtc cccgaagtga
    40561 tgggaaaagt cttagcggca atgcagaata gaaagtgctc atcttttatt tcccgggagg
    40621 ggaaatgtcc cgcgtatcgc aggcgaggaa cctgggtaaa tatttcctgc tcatcgataa
    40681 tatgctggtc gtgctggggt tctttgttgt cttcccgctg atctctatcc gcttcgttga
    40741 tcaaatgggc tgggccgccg tcatggtcgg tattgctctc ggtctacgcc agtttattca
    40801 gcaaggtctg ggtattttcg gcggcgcaat tgccgaccgc tttggtgcca aaccgatgat
    40861 tgttaccggt atgctgatgc gcgccgccgg attcgccaca atgggtatcg cccacgaacc
    40921 gtggctattg tggttttcat gcctgctctc gggactcggc ggcacgttgt ttgatccgcc
    40981 gcgttcggcg ctggtggtga aactaatccg tccacaacaa cgtggtcgtt ttttctcgct
    41041 gttgatgatg caggacagtg ccggtgcggt cattggcgca ttgttgggga gctggctgtt
    41101 gcaatacgac tttcgcctgg tctgcgccac aggggccgtt ttgtttgtgc tgtgtgcggc
    41161 gttcaatgcg tggttgttac cggcatggaa actctccacc gtacgcacgc ccgttcgcga
    41221 aggcatgacc cgcgtgatgc gtgacaagcg ttttgtcacc tatgtcctga cgctggcggg
    41281 ttactacatg ctggctgtac aagtgatgct gatgctgcca attatggtca acgacgtggc
    41341 tggcgcaccc tctgccgtta aatggatgta tgccattgaa gcgtgtctgt cgttaacgtt
    41401 gctctaccct atcgcccgct ggagtgaaaa gcattttcgt ctggaacacc ggttgatggc
    41461 tgggctgttg ataatgtcat taagcatgat gccggtgggc atggtcagcg gcctgcaaca
    41521 acttttcacc ctgatttgtc tgttttatat cgggtcgatc attgccgagc ctgcgcgtga
    41581 aaccttaagt gcttcgctgg cggacgcaag agctcgcggc agctatatgg ggtttagccg
    41641 tctgggtctg gcgattggcg gcgctattgg ttatatcggt ggtggctggc tgtttgacct
    41701 gggcaaatcg gcgcaccagc cagagcttcc gtggatgatg ctgggcatta ttggcatctt
    41761 cactttcctt gcgctgggtt ggcagtttag ccagaaacgc gccgcgcgtc gtttgcttga
    41821 acgcgacgcc tgatttgata ctcccccctg actcctccat actgaaagtg aactgacagc
    41881 caaatggagg agtcacgtga agctatacat ttacgatcac tgcccttact gcctcaaagc
    41941 ccgcatgatt ttcggcctga aaaatatccc cgtcgaatta catgttctgc tcaacgacga
    42001 cgcagaaaca cccccccgga tggtcggtca aaaacaggtt cccattctgc aaaaagatga
    42061 cagccgctat atgccagaaa gcatggacat cgttcactat gtcgataaac tcgacggcaa
    42121 accgttactg accggcaaac gttcccctgc cattgaagag tggctgcgca aggtcaatgg
    42181 ctacgcaaac aaactgctgt tgccgcgttt tgccaaatcg gcatttgatg agttttctac
    42241 tcccgccgcg cgcaaatatt tcgtcgacaa gaaagaggcc agcgcgggta atttagccga
    42301 cctgctggcc cactctgacg gtctgattaa gaatatcagc gatgatttgc gtgcgctgga
    42361 caaactgatc gtcaaaccga acgccgtgaa tggcgaactt tcggaagatg atattcagct
    42421 atttccgcta ctgcgtaatc tgacgctggt agccggaatc aactggccaa gccgcgttgc
    42481 tgattaccgc gataatatgg cgaaacagac acaaatcaat ttgttatcat caatggcgat
    42541 ttaatcctga cccgccgcgg cagcctgtat tcgcgggttg ccgccgtttt cagttttttc
    42601 tgctggcgag tttatcgctg gtgacacatg ctgcgttgtg tcaggatatt gacgcagatt
    42661 tcatgtcgag gactaccatg aacaagtttt tatttgccgc tgcattgatc gtcagtggcc
    42721 tgctcgttgg ctgtaatcaa ctcacccaat acaccatcac cgaacaagaa attaaccagt
    42781 cgcttgcgaa acataataat ttctcaaaag atatcggttt acccggcgtg gctgacgccc
    42841 atattgttct gacaaacttg accagccaaa ttggtcgcga agagccgaat aaggttaccc
    42901 taaccggaga cgccaatctg gacatgaact ccctgttcgg tagtcagaaa gcgaccatga
    42961 aactgaagct gaaagcgctg cccgtgtttg ataaagaaaa aggtgcgatc ttcctgaaag
    43021 agatggaagt ggtcgatgcg acggtacaac cggaaaaaat gcaaacggtg atgcaaacgt
    43081 tgcttcccta tttgaaccag gcattacgca attactttaa ccagcaacct gcttacgtcc
    43141 tgcgcgaaga tggcagtcag ggcgaagcaa tggcgaaaaa actggcgaaa ggcattgaag
    43201 tgaagccagg cgaaattgtc attccgttta cggattaatg acgagggcgc attcgcgccc
    43261 tttatttttc gtgcaaagga aaacgtttcc gcttatcctt tgtgtccggc aaaaacatcc
    43321 cttcagccgg agcatagaga ttaatgactg caccatccca ggtattaaag atccgccgcc
    43381 cagacgactg gcaccttcac ctccgcgatg gcgacatgtt aaaaactgtc gtgccgtata
    43441 ccagcgaaat ttatggacgg gctattgtaa tgcccaatct ggctccgccc gtgaccactg
    43501 ttgaggctgc cgtggcgtat cgccagcgta ttcttgatgc cgtacctgcc gggcacaatt
    43561 tcaccccatt gatgacctgt tatttaacag attcgctgga tcctaatgag ctggagcgcg
    43621 gatttaacga aggcgtgttc accgctgcaa aactttatcc ggcaaacgca accactaact
    43681 ccagccacgg cgtgacgtca attgacgcaa tcatgccggt acttgagcgc atggaaaaaa
    43741 tcggtatgcc gctactggtg catggtgaag tgacacatgc agatatcgac atttttgatc
    43801 gtgaagcgcg ctttatagaa agcgtgatgg aacctctgcg ccagcgcctg actgcgctga
    43861 aagtcgtttt tgagcacatc accaccaaag atgctgccga ctatgtccgt gacggaaatg
    43921 aacggctggc tgccaccatc actccgcagc atctgatgtt taaccgcaac catatgctgg
    43981 ttggtggcgt gcgtccgcac ctgtattgtt tacccatcct caaacgcaat attcaccaac
    44041 aggcattgcg tgaactggtc gccagcggtt ttaatcgagt attcctcggt acggattctg
    44101 cgccacatgc acgtcatcgc aaagagagca gttgcggctg cgcgggctgc ttcaacgccc
    44161 caaccgcgct gggcagttac gctaccgtct ttgaagagat gaatgctctg cagcactttg
    44221 aagcattctg ttctgtaaac ggcccgcagt tctatggctt gccggtcaac gacacattca
    44281 tcgaactggt acgtgaagag caacaggttg ctgaaagcat cgcactgact gatgacaccc
    44341 tggtgccatt cctcgccggg gaaacggtac gctggtccgt taaacaataa aaatcgtagc
    44401 ttcctgttgt cattaggtta ttttacctgt ataaataacc agtatattca acagggggct
    44461 attatgcgaa ttgaagtcac catagcgaaa acttctccat tgccagctgg ggctattgac
    44521 gccctggctg gcgaactttc ccgccgtatt cagtatgcgt ttcctgataa tgaaggccac
    44581 gtatcggtac gttatgccgc agcgaataat ttatcggtta ttggcgcaac aaaagaagat
    44641 aaacagcgca ttagcgaaat tctccaggaa acgtgggaaa gcgccgatga ctggtttgtc
    44701 agcgaataat atgcagtgat ttttgttttt gccgggtcgc cccggctttt ttattttact
    44761 tacaaatttt gagtacaaaa tttctacact tcctcaaaat
//
LOCUS       DAZGLJ010000015        44506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000015 DAZGLJ010000000
VERSION     DAZGLJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.015"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(53..1417)
                     /gene="mnmE"
                     /locus_tag="ACHK49_000747"
     CDS             complement(53..1417)
                     /gene="mnmE"
                     /locus_tag="ACHK49_000747"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO9261437.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLYDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(1523..3169)
                     /gene="yidC"
                     /locus_tag="ACHK49_000748"
     CDS             complement(1523..3169)
                     /gene="yidC"
                     /locus_tag="ACHK49_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO9261438.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3172..3429)
                     /gene="yidD"
                     /locus_tag="ACHK49_000749"
     CDS             complement(3172..3429)
                     /gene="yidD"
                     /locus_tag="ACHK49_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO9261439.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3393..3752)
                     /gene="rnpA"
                     /locus_tag="ACHK49_000750"
     CDS             complement(3393..3752)
                     /gene="rnpA"
                     /locus_tag="ACHK49_000750"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO9261440.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3769..3909)
                     /gene="rpmH"
                     /locus_tag="ACHK49_000751"
     CDS             complement(3769..3909)
                     /gene="rpmH"
                     /locus_tag="ACHK49_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO9261441.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4139..4219)
                     /locus_tag="ACHK49_000752"
     CDS             complement(4139..4219)
                     /locus_tag="ACHK49_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9261442.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            4516..5919
                     /gene="dnaA"
                     /locus_tag="ACHK49_000753"
     CDS             4516..5919
                     /gene="dnaA"
                     /locus_tag="ACHK49_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO9261443.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNIPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            5924..7024
                     /gene="dnaN"
                     /locus_tag="ACHK49_000754"
     CDS             5924..7024
                     /gene="dnaN"
                     /locus_tag="ACHK49_000754"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO9261444.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7024..8097
                     /gene="recF"
                     /locus_tag="ACHK49_000755"
     CDS             7024..8097
                     /gene="recF"
                     /locus_tag="ACHK49_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO9261445.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8126..10540
                     /gene="gyrB"
                     /locus_tag="ACHK49_000756"
     CDS             8126..10540
                     /gene="gyrB"
                     /locus_tag="ACHK49_000756"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO9261446.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            10780..11178
                     /gene="yidB"
                     /locus_tag="ACHK49_000757"
     CDS             10780..11178
                     /gene="yidB"
                     /locus_tag="ACHK49_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO9261447.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            11293..12105
                     /gene="yidA"
                     /locus_tag="ACHK49_000758"
     CDS             11293..12105
                     /gene="yidA"
                     /locus_tag="ACHK49_000758"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO9261448.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMELPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12151..12807)
                     /gene="yidX"
                     /locus_tag="ACHK49_000759"
     CDS             complement(12151..12807)
                     /gene="yidX"
                     /locus_tag="ACHK49_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO9261449.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYHWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13085..13774
                     /gene="dgoR"
                     /locus_tag="ACHK49_000760"
     CDS             13085..13774
                     /gene="dgoR"
                     /locus_tag="ACHK49_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO9261450.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            13771..14649
                     /gene="dgoK"
                     /locus_tag="ACHK49_000761"
     CDS             13771..14649
                     /gene="dgoK"
                     /locus_tag="ACHK49_000761"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO9261451.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            14633..15250
                     /gene="dgoA"
                     /locus_tag="ACHK49_000762"
     CDS             14633..15250
                     /gene="dgoA"
                     /locus_tag="ACHK49_000762"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO9261452.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            15247..16395
                     /gene="dgoD"
                     /locus_tag="ACHK49_000763"
     CDS             15247..16395
                     /gene="dgoD"
                     /locus_tag="ACHK49_000763"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO9261453.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            16470..17807
                     /gene="dgoT"
                     /locus_tag="ACHK49_000764"
     CDS             16470..17807
                     /gene="dgoT"
                     /locus_tag="ACHK49_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9261454.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17804..18868)
                     /gene="cbrA"
                     /locus_tag="ACHK49_000765"
     CDS             complement(17804..18868)
                     /gene="cbrA"
                     /locus_tag="ACHK49_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO9261455.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEIYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKRIMRSGMAHIPQLKD"
     gene            18969..20183
                     /gene="yidR"
                     /locus_tag="ACHK49_000766"
     CDS             18969..20183
                     /gene="yidR"
                     /locus_tag="ACHK49_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO9261456.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYMPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAEVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(20185..20517)
                     /gene="yidQ"
                     /locus_tag="ACHK49_000767"
     CDS             complement(20185..20517)
                     /gene="yidQ"
                     /locus_tag="ACHK49_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO9261457.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    20757..20829
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            20823..21236
                     /gene="ibpA"
                     /locus_tag="ACHK49_000768"
     CDS             20823..21236
                     /gene="ibpA"
                     /locus_tag="ACHK49_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO9261458.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            21348..21776
                     /gene="ibpB"
                     /locus_tag="ACHK49_000769"
     CDS             21348..21776
                     /gene="ibpB"
                     /locus_tag="ACHK49_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO9261459.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            21973..23634
                     /gene="yidE"
                     /locus_tag="ACHK49_000770"
     CDS             21973..23634
                     /gene="yidE"
                     /locus_tag="ACHK49_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO9261460.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDMPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(23631..24347)
                     /gene="yidP"
                     /locus_tag="ACHK49_000771"
     CDS             complement(23631..24347)
                     /gene="yidP"
                     /locus_tag="ACHK49_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO9261461.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            24643..26259
                     /locus_tag="ACHK49_000772"
     CDS             24643..26259
                     /locus_tag="ACHK49_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO9261462.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            26259..27073
                     /locus_tag="ACHK49_000773"
                     /pseudo
     CDS             26259..27073
                     /locus_tag="ACHK49_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27070..27963)
                     /gene="yidL"
                     /locus_tag="ACHK49_000774"
     CDS             complement(27070..27963)
                     /gene="yidL"
                     /locus_tag="ACHK49_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO9261463.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            28130..29845
                     /gene="yidK"
                     /locus_tag="ACHK49_000775"
     CDS             28130..29845
                     /gene="yidK"
                     /locus_tag="ACHK49_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO9261464.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            29842..31335
                     /gene="yidJ"
                     /locus_tag="ACHK49_000776"
     CDS             29842..31335
                     /gene="yidJ"
                     /locus_tag="ACHK49_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO9261465.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDGLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(31382..31831)
                     /gene="yidI"
                     /locus_tag="ACHK49_000777"
     CDS             complement(31382..31831)
                     /gene="yidI"
                     /locus_tag="ACHK49_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO9261466.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            31941..32288
                     /gene="yidH"
                     /locus_tag="ACHK49_000778"
     CDS             31941..32288
                     /gene="yidH"
                     /locus_tag="ACHK49_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO9261467.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            32278..32640
                     /gene="yidG"
                     /locus_tag="ACHK49_000779"
     CDS             32278..32640
                     /gene="yidG"
                     /locus_tag="ACHK49_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO9261468.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            32637..33134
                     /gene="yidF"
                     /locus_tag="ACHK49_000780"
     CDS             32637..33134
                     /gene="yidF"
                     /locus_tag="ACHK49_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO9261469.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(33142..34326)
                     /gene="emrD"
                     /locus_tag="ACHK49_000781"
     CDS             complement(33142..34326)
                     /gene="emrD"
                     /locus_tag="ACHK49_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO9261470.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            34408..34482
                     /gene="ysdE"
                     /locus_tag="ACHK49_000782"
     CDS             34408..34482
                     /gene="ysdE"
                     /locus_tag="ACHK49_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO9261471.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(34745..34834)
                     /gene="tisB"
                     /locus_tag="ACHK49_000783"
     CDS             complement(34745..34834)
                     /gene="tisB"
                     /locus_tag="ACHK49_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO9261472.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            35399..35497
                     /gene="ivbL"
                     /locus_tag="ACHK49_000784"
     CDS             35399..35497
                     /gene="ivbL"
                     /locus_tag="ACHK49_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO9261473.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            35603..37291
                     /gene="ilvB"
                     /locus_tag="ACHK49_000785"
     CDS             35603..37291
                     /gene="ilvB"
                     /locus_tag="ACHK49_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO9261474.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDVFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            37295..37585
                     /gene="ilvN"
                     /locus_tag="ACHK49_000786"
     CDS             37295..37585
                     /gene="ilvN"
                     /locus_tag="ACHK49_000786"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO9261475.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            37660..38250
                     /gene="uhpA"
                     /locus_tag="ACHK49_000787"
     CDS             37660..38250
                     /gene="uhpA"
                     /locus_tag="ACHK49_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO9261476.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            38250..39752
                     /gene="uhpB"
                     /locus_tag="ACHK49_000788"
     CDS             38250..39752
                     /gene="uhpB"
                     /locus_tag="ACHK49_000788"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO9261477.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            39762..41081
                     /gene="uhpC"
                     /locus_tag="ACHK49_000789"
     CDS             39762..41081
                     /gene="uhpC"
                     /locus_tag="ACHK49_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO9261478.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGVSALLLLPFLNAQAPREA"
     gene            41337..42728
                     /gene="uhpT"
                     /locus_tag="ACHK49_000790"
     CDS             41337..42728
                     /gene="uhpT"
                     /locus_tag="ACHK49_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO9261479.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(42773..>44506)
                     /gene="adeD"
                     /locus_tag="ACHK49_000791"
     CDS             complement(42773..>44506)
                     /gene="adeD"
                     /locus_tag="ACHK49_000791"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO9261480.1"
                     /translation="ISRAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIK
                     GRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLT
                     TVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQM
                     LAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITA
                     GIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRN
                     PWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVL
                     LSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFT
                     PGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRLAPACGLLGGFG
                     LNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIA
                     GLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGE
                     KFAFTTLEVTE"
BASE COUNT        10241 a        11454 c        12100 g        10711 t
ORIGIN      
        1 acaatgaagc gaagccgcat ctgacagtca gaatacggct tcgtaagcgc ggttacttac
       61 caatacagaa gctggagaaa atccgcccca gcaggtcgtc tgaagtaaat tccccggtga
      121 tttcgcttaa gttctgctgt gccaggcgca actcttccgc cagcagttca cccgcccagg
      181 cacctaacag ttgcgcttta ccttgctgca aatgctccgc tgcctgttcc agcgcctgta
      241 agtggcgacg gcgcgccagg aaaccgcctt ccatgttggt gtcaaagccc atgctctgtt
      301 tgagatggtt acgcagcacg tccacgcctt caccagtcct tgccgagaga cgaattaacg
      361 cgtgaccgtt cacttcactc attcccagcg tttcgccggt gatatcggct ttattgcgca
      421 ccacggtgat cggcagtttc gctggcagac gggcgataaa ttccggccag atctctgccg
      481 gatccacggc gtctgttgtg gtgccatcga ccataaacag cacccggtcg gcctgttcaa
      541 tttcctgcca cgcgcgctcg atcccaatac gttccacttc gtcactggct tcacgtagcc
      601 cggcggtgtc gatgatatgc agcggcattc cgtcaatgtg gatatgctca cgcaacacgt
      661 cacgcgtagt tccggcgata tcggttacga ttgctgcttc acggccagcc agcgcgttta
      721 acaggctcga tttaccggcg ttaggacgtc cggcaatcac cactttcatc ccttcgcgca
      781 acaaactacc ctgacgtgct tcagcacgca ctgcatcaag atcggcaata acgtcattga
      841 gctgggcttc aattttgccg tcggagagga aatcgatctc ttcatcaggg aaatcaattg
      901 ccgcttcgac gtaaatgcgc aagtgggtga gggcttctac cagatgatta acccgtgcgg
      961 agaatgcgcc ttgcagcgag ttaagtgccg aacgggccgc ctgttccgag ctggcgtcga
     1021 taagatcggc aatcgcctca gcctgggcta agtcgagttt atcgtaaaga aacgcgcgtt
     1081 cggaaaactc accagggcga gcaatccgca ggccgggaat ggtcagaatg cgttttaaca
     1141 gcaggtcgag gatcaccgga ccgccatgac cctgcagttc aagcacatct tcgccggtga
     1201 acgagttcgg gccagggaac catagcgcaa tcccctgatc gagcacgctg ccgtcggcgt
     1261 ctttaaatgg caaataatcg gcgtagcgcg gcttaggtag tttacccagc acggtttcgg
     1321 caacttcacg ggctttgagg ccggagatgc gcaggatgcc aacaccgcca cgtcccggag
     1381 gcgtggcctg ggctacgata gtgtcattat cgctcatgat gttcctgttg ctttgtgtgg
     1441 cggatgcgcg gtgcttatcc gccctacgaa aagaaaaaag gcggtcaact gaccgccctt
     1501 attttagcga aaactcaccg aatcaggatt ttttcttctc gcggctatgc aggccacgtt
     1561 tttccagacc acggtaaatc agctgctgct gaataatggt taccaggttg ctgacgatat
     1621 agtacagcac cagacctgac gggaaccaca ggaagaacac ggtgaagatg accggcataa
     1681 aggtcatgat cttctgctgc atcgggtcgg tcactgtggt cggcgacatc ttctgaatga
     1741 agaacatcgt tacgcccatc aggatcggca ggatgtagta cgggtcctgt gccgacaggt
     1801 cgtggatcca cagtgcaaac ggtgcctgac gcagttcaac ggaacccatc agcatgtagt
     1861 acaacgccag gaagattggc atctggatca gcagcgggaa gcagccgccc agcgggttaa
     1921 ccttctcagc tttgtacagc gccatcattt cctggctgat acgctgtttg tcatcgccca
     1981 gacgctcacg cattgcctga atcttcggct gcagcatacg catcttcgcc atggaggtgt
     2041 actgcgcttt ggtcagcggg tacatgatgc cacgaacgat aaaggtgatg atgataatgg
     2101 agaagcccca gttacccaca aagctatgga tccatttcag cagtttgaac agcggctgag
     2161 agatgaacca caaccaaccg taatcaacgg tcagatccag gtgcggagca acagctgcca
     2221 ttttgtcctg gatttccggg ccaacccaca gggtgctgtt catcgcgcca gtctgaccag
     2281 gctgaaccag taccggctga gatttatagc cgatagcggc gatgccgtta cccagattag
     2341 cggtatagaa gttgttggta ccgtcgttat gcgggatcca cgccgtcgcg aaatactgtt
     2401 gcagcatcgc cacccaacca cctttcgaag agatgttcag gttttcgtta tcggcaatgg
     2461 tatcgaactt gtatttctca tacttctcgt caggcgtgga gtacgccgcg ccacggaagg
     2521 tgtgcagtgc gaagttgctg cttccggtat cgagatgcgg tggcagagtg atggattgct
     2581 tcaactgacc aaaggtggag atttccagcg gtttctcgcc agcgttctgc acgttgtagt
     2641 tgacgttgac agcgtaatca ccacgtttca ggacaaacgt tttggtaaac gtgttgcctg
     2701 ccgcgtcggt atacgtcatc ggcacctgca gttcgttttg accttcagcc agcacataag
     2761 cgtctttttc aacgttatac agcggacgcg ggccgttagc cgggttatcc gggccatcac
     2821 gaccggtcag accgctctgt gcctgataaa taaactgcgg tgaagtttcc agcagctgga
     2881 acggctgggt agagttcagc tctttcgggt aagcaggcag cagagcttgc tcaacatcac
     2941 caccacgggt gttgatggtc agatcaagca cgtcggtctt aaccgagatc agtttcccct
     3001 ggccactggc cggtacgccc tggtcggcgg cgctacccgc tgcggtggtc gttgtctgcg
     3061 tggtctgttg ggcctgaggt tgcgggtttt tatcctgctc ccaggcttgc cagatcatga
     3121 aagacacgaa cagcaaagcg atgactaaaa gattgcgttg cgaatccatc gttagtgttc
     3181 tctggtatca aatggtccgg gcgggacggg atcgtcacca ccagggtgta aagggtggca
     3241 ttttaatacg cgtttcaccg tcaaccaact gccttttatc actccaaacc tgcgcaatgc
     3301 ctcaattccg tagcttgaac aggttggagt gaaacgacaa tgcggcccga gtagcggact
     3361 aatcaggcgt tgatagaccc gaatgagggc tatcaggacc cgcgagccag gcgacagtgg
     3421 cggcgccata atttttccaa cgcttccgag agagcacggt tatcgaggtc ggcaacccct
     3481 tttttcgcca ccaccacgaa atccatagcc gggagttcat gttggcgcag acggaagctt
     3541 tcacgcgtca gacgtttaat ccgattgcgt tcatgggcgc gtcgaacgtt tttcttggcg
     3601 actgtaagac cgatacgggg atgccccagc gaattcaggc ggccgagaat ggtaatttgc
     3661 ggcgtgccag cccgttgtgg ctgctggaag acgaatgtga attgactggg agttaacaag
     3721 cgtaactccc tgggaaatgc gagcttaacc actcaggggt tagctttatt acttagaaac
     3781 ggtcagacga gcgcggcctt tagcacgacg acgtgccaga acctgacgac catttttagt
     3841 agccatacga gcacggaagc cgtgagaacg gttgcgcttc agtacagacg gttgaaaagt
     3901 gcgtttcatg gcgatttcta cctaaacttg aataaattca atggctttat tggatatccg
     3961 ccgaaaaatg aaacgatgga caccgaagcc atgggtgatt aaagaggccg gattgtaata
     4021 attgtacact ccggagtcaa ttctctttcc ttatttaccg cgcttttccg caccttttcg
     4081 cagggaaaat gtacgacctc acaccagtga aaaccagcat ggcgcgccgg gtggaggatt
     4141 atacgggctg atgggtaaag cgcaaggatc gtcctggatc tttattagat cgattaagcc
     4201 aatttttgtc tatggtcatt aaattttcca atatgcggcg taaatcgtgc ccgcctcgcg
     4261 gcaggatcgt ttacacttag cgaggtctgg aaagtcctgt ggataaatcg ggaaaatctg
     4321 tgagaaacag aagatctctt gcgcagttta ggctatgatc cgcggtcccg atcgttttgc
     4381 aggatcttga tcgggcatat aaccgcagac agcggttcgt gcgtcaccct caagcagggt
     4441 cttttcgacg tacgtcaaca atcatgaatg tttcagcctt agtcattatc gacttttgtt
     4501 cgagtggagt ccgccgtgtc actttcgctt tggcagcagt gtcttgcccg attgcaggat
     4561 gagttaccag ccacagaatt cagtatgtgg atacgcccat tgcaggcgga actgagcgat
     4621 aacacgctgg ccctgtacgc gccaaaccgt tttgtcctcg attgggtacg ggacaagtac
     4681 cttaataata tcaatggact gctaaccagt ttctgcggag cggatgcccc acagctgcgt
     4741 tttgaagtcg gcaccaaacc ggtgacgcaa acgccacaag cggcagtgac gagcaacgtc
     4801 gcggcccctg cacaggtggc gcaaacgcag ccgcaacgtg ctgcgccttc tacgcgctca
     4861 ggttgggata acatcccggc cccggcagaa ccgacctatc gttctaacgt aaacgtcaaa
     4921 cacacgtttg ataacttcgt tgaaggtaaa tctaaccaac tggcgcgcgc ggcggctcgc
     4981 caggtggcgg ataaccctgg cggtgcctat aacccgttgt tcctttatgg cggcacgggt
     5041 ctgggtaaaa ctcacctgct gcatgcggtg ggtaacggca ttatggcgcg caagccgaat
     5101 gccaaagtgg tttatatgca ctccgagcgc tttgttcagg acatggttaa agccctgcaa
     5161 aacaacgcga tcgaagagtt taaacgctac taccgttccg tagatgcact gctgatcgac
     5221 gatattcagt tttttgctaa taaagaacga tctcaggaag agtttttcca taccttcaac
     5281 gccctgctgg aaggtaatca acagatcatt ctcacctcgg atcgctatcc gaaagagatc
     5341 aacggcgttg aggatcgttt gaaatcccgc ttcggttggg gactgactgt ggcaatcgaa
     5401 ccgccagagc tggaaacccg tgtggcgatc ctgatgaaaa aggccgacga aaacgacatt
     5461 cgtttgccgg gcgaagtggc gttctttatc gccaagcgtc tacgatctaa cgtacgtgag
     5521 ctggaagggg cgctgaaccg cgtcattgcc aatgccaact ttaccggacg ggcgatcacc
     5581 atcgacttcg tgcgtgaggc gctgcgcgac ttgctggcat tgcaggaaaa actggtcacc
     5641 atcgacaata ttcagaagac ggtggcggag tactacaaga tcaaagtcgc ggatctcctt
     5701 tccaagcgtc gatcccgctc ggtggcgcgt ccgcgccaga tggcgatggc gctggcgaaa
     5761 gagctgacta accacagtct gccggagatt ggcgatgcgt ttggtggtcg tgaccacacg
     5821 acggtgcttc atgcctgccg taagatcgag cagttgcgtg aagagagcca cgatatcaaa
     5881 gaagattttt caaatttaat cagaacattg tcatcgtaaa cctatgaaat ttaccgtaga
     5941 acgtgagcat ttattaaaac cgctacaaca ggtgagcggt ccgttaggtg gtcgtcctac
     6001 gctaccgatt ctcggtaatc tgctgttaca ggttgctgac ggtacgttgt cgctgaccgg
     6061 tactgatctc gagatggaaa tggtggcacg tgttgcgctg gttcagccac acgagccagg
     6121 agcgacgacc gttccggcgc gcaaattctt tgatatctgc cgtggtctgc ctgaaggcgc
     6181 ggaaattgcc gtgcagctgg aaggtgaacg gatgctggta cgctccgggc gtagccgttt
     6241 ttcgctgtct accctgccag cggcggattt cccgaacctc gatgactggc agagtgaagt
     6301 cgaatttacc ctgccgcagg caacgatgaa gcgtctgatt gaagcgaccc agttttcgat
     6361 ggcgcatcag gacgttcgct attacttaaa tggtatgctg tttgaaaccg aaggtgaaga
     6421 actgcgcacc gtggcaaccg acggccaccg tctggcggtc tgttcaatgc caattggtca
     6481 atctttgcca agccattcgg tgatcgtacc gcgtaaaggc gtgattgaac tgatgcgtat
     6541 gctcgacggc ggcgacaatc cgctgcgcgt gcagattggc agcaacaata ttcgcgccca
     6601 cgttggcgac tttatcttca cctccaaact ggtggatggt cgcttcccgg attaccgccg
     6661 cgttctgccg aagaatccgg acaaacatct ggaagctggc tgcgatctgc tcaagcaggc
     6721 gtttgcccgt gcggcaattc tctctaacga gaaattccgc ggcgtgcgcc tgtatgtcag
     6781 cgaaaaccag ctgaaaatca ccgccaacaa cccggaacag gaagaagcgg aagagatcct
     6841 cgacgttacc tatagcggtg cggagatgga aatcggcttc aacgtcagct atgtgctgga
     6901 tgttctgaac gcgctgaaat gcgaaaacgt ccgcatgatg ctgaccgatt cggtttccag
     6961 cgtgcagatt gaagatgccg catcacagtc ggctgcctat gttgtcatgc caatgagact
     7021 gtaatgtccc tcacccgctt gttgatccgc gatttccgca acattgaaac cgcggatctc
     7081 gctttatctc ccggctttaa ctttctggta ggtgccaacg gcagtggcaa aaccagcgtg
     7141 ctggaagcca tctatacgct cggccatggt cgggcgtttc gcagtttgca gattggtcgc
     7201 gtcattcgcc atgagcagga ggcatttgtt ctccatgggc gattacaggg cgaagagcgc
     7261 gagacggcga ttggcttaac caaggacaaa cagggcgaca gcaaagtccg catcgacggt
     7321 actgacgggc ataaagtcgc ggaactggcg cacctgatgc caatgcagct gataacgcca
     7381 gaagggttta ctttactcaa cggcggcccc aaatacagaa gagcattcct cgactgggga
     7441 tgctttcaca acgaacccgg atttttcacc gcctggagca atctcaagcg attgctcaag
     7501 cagcgcaatg cggcgctgcg ccaggtgaca cgttacgaac agctacgccc gtgggataaa
     7561 gaactgatcc cgctggcgga gcaaatcagc acctggcgcg cggagtatag cgccggtatc
     7621 gcggccgata tggccgatac ctgtaagcaa tttctccctg agttttctct gactttctct
     7681 ttccagcgcg gctgggagaa agagacagaa tatgctgagg tgctggaacg taattttgaa
     7741 cgcgatcgcc agctaaccta caccgcgcat ggcccgcata aagcggactt acgcattcgc
     7801 gccgacggtg cgccggtgga agatacctta tcgcgtgggc agcttaagct gttgatgtgc
     7861 gccttacgtc tggcgcaagg agagttcctc acccgtgaaa gcgggcggcg gtgtctctac
     7921 ctgatagatg attttgcctc tgagcttgat gatgagcgtc gtgggttgct tgccagccgc
     7981 ttaaaagcga cgcaatcaca ggtctttgtc agcgcgatca gtgctgaaca cgttatagac
     8041 atgtcggacg aaaattcgaa gatgtttacc gtggaaaagg gtaaaataac ggattaaccc
     8101 aagtataaat gagcgagaaa cgttgatgtc gaattcttat gactcctcca gtatcaaagt
     8161 cctgaaaggg ctggatgcgg tgcgtaagcg cccgggtatg tatatcggcg acacggatga
     8221 cggcaccggt ctgcaccaca tggtattcga ggtggtagat aacgctatcg acgaagcgct
     8281 cgcgggtcac tgtaaagaaa ttatcgtcac cattcacgcc gacaactctg tctctgtaca
     8341 ggatgacggg cgcggcattc cgaccggtat tcacccggaa gagggcgtat cggcggcgga
     8401 agtgatcatg accgttctgc acgcaggcgg taaattcgac gataactcct ataaagtgtc
     8461 cggcggtctg cacggcgttg gtgtttcggt agtaaacgcc ctgtcgcaaa aactggagct
     8521 ggttatccag cgcgagggta aaattcaccg tcagatctac gaacacggtg taccgcaggc
     8581 cccgctggcg gttaccggcg agactgaaaa aaccggcacc atggtgcgtt tctggcctag
     8641 cctcgaaact ttcaccaatg tgaccgagtt cgaatatgaa attctggcga aacgtctgcg
     8701 tgagttgtcg ttcctcaact ccggcgtttc cattcgtctg cgcgacaagc gcgacggcaa
     8761 agaagaccac ttccactatg aaggcggcat caaggcgttc gttgaatatc tgaacaagaa
     8821 caaaacgccg atccacccga atatcttcta cttctccact gaaaaagacg gtattggcgt
     8881 cgaagtggcg ttgcagtgga acgatggctt ccaggaaaac atctactgct ttaccaacaa
     8941 cattccgcag cgtgacggcg gtactcacct ggcaggcttc cgtgcggcga tgacccgtac
     9001 cctgaacgcc tacatggaca aagaaggcta cagcaaaaaa gccaaagtta gcgccaccgg
     9061 tgacgatgcg cgtgaaggcc tgattgcggt cgtttccgtg aaagtgccgg acccgaaatt
     9121 ctcctcccag accaaagaca aactggtttc ttctgaggtg aaatcagcgg ttgaacagca
     9181 gatgaacgaa ctgctggcag aatacctgct ggaaaaccca accgacgcga aaatcgtggt
     9241 tggcaaaatt atcgatgctg cccgtgcccg tgaagcggcg cgtcgcgcgc gtgaaatgac
     9301 ccgccgtaaa ggtgcgctcg acttagcggg cctgccgggc aaactggcag actgccagga
     9361 acgcgatccg gcgctttccg aactgtacct ggtggaaggg gactccgcgg gcggctctgc
     9421 gaagcagggg cgtaaccgca agaaccaggc gattctgccg ctgaagggta aaatcctcaa
     9481 cgtcgagaaa gcgcgcttcg ataagatgct ctcttctcag gaagtggcga cgcttatcac
     9541 cgcgcttggc tgtggtatcg gtcgtgacga gtacaacccg gacaaactgc gttatcacag
     9601 catcatcatc atgaccgatg cggacgtcga cggctcgcac attcgtacgc tgctgttgac
     9661 cttcttctat cgtcagatgc cggaaatcgt tgaacgcggt cacgtctaca tcgctcagcc
     9721 gccgctgtac aaagtgaaga aaggcaagca ggaacagtac attaaagacg acgaagcgat
     9781 ggatcagtac cagatctcta tcgcgctgga tggcgcaacg ctgcacacca acgccagcgc
     9841 accggcattg gctggcgaag cgttagagaa attggtgtct gagtacaacg cgacgcagaa
     9901 aatgatcaac cgcatggagc gtcgttatcc gaaagcaatg ctgaaagagc ttatctatca
     9961 gccgacgctg acggaagccg acctctctga tgagcagacc gttacccgct gggtgaacgc
    10021 gctggtcagc gaactgaacg acaaagaaca gcacggcagc cagtggaagt ttgatgtcca
    10081 caccaatgcc gaacaaaacc tgttcgagcc gattgttcgc gtgcgtaccc acggtgtgga
    10141 tactgactat ccgctggatc acgagtttat caccggtggc gaatatcgtc gtatctgcac
    10201 gctgggtgag aaactgcgtg gcttgctgga agaagatgcg tttatcgaac gtggcgagcg
    10261 tcgtcagccg gtagccagct tcgagcaggc gctggactgg ctggtgaaag agtcccgtcg
    10321 cggcctctcc atccagcgtt ataaaggtct gggcgagatg aacccggaac agctgtggga
    10381 aaccaccatg gacccggaaa gccgtcgtat gctgcgcgtt accgttaagg atgcgattgc
    10441 cgctgaccag ttgttcacca cgctgatggg cgacgccgtt gaaccgcgcc gtgcgtttat
    10501 tgaagagaac gccctgaaag cggcgaatat cgatatttaa tggcgctaac catgcgagcg
    10561 tgcctgatgc gctacgctta tcaggcctac gaaaatcttg caatgtattg aattttcatg
    10621 attttgtagg ccggataagg cgttcacgcc gcatccggca agaagctata agaaaagggc
    10681 ggagatcatc tccgcccttt ttatttctgc aatccgcaat tatcatcccg caaatctata
    10741 ctttttgggt caacacgtaa cgctttacag gtgattatca tggggctttt tgatgaagtt
    10801 gtcggtgcct ttctgaaagg cgatgcgggg aaatatcagg ctattttaag ttgggttgag
    10861 gagcagggcg gcattcaggt gctgctggaa aaactgcaaa gtggcggctt aggggccatt
    10921 ctctcaacct ggctgagtaa tcaacagggc aatcaatcgg ttagtggcga gcaactggaa
    10981 tcggcgctcg gcacaaatgc ggtgtccgat cttggacaaa aacttggcgt ggataccagt
    11041 acagcttcca gtttactggc agaacaattg ccgaagatta ttgatgcgct ctcaccgcaa
    11101 ggtgaagtgt ctccacaagc caataacgat ctgctttccg caggcatgga actgctgaaa
    11161 gggaaactct tccgctaagc aaatggggag catgcaaccc gcgttgcatc ccccttttcc
    11221 ctcgggtgtt gtactgattt ttgagcggaa tcgcgttagc atgggtcagg aactaatcta
    11281 cctggggaac tcatggctat caaactcatt gctatcgata tggatggcac ccttctgcta
    11341 cccgatcaca ccatttcacc cgccgttaaa aatgcgattg ccgcagctcg cgcccgtggc
    11401 gtgaatgtcg tgctaacgac gggtcgccct tatgcaggtg ttcacaatta cctgaaagag
    11461 ctgcatatgg aactgccggg cgactactgc attacttata acggcgcgct ggtacagaag
    11521 gccgctgatg gtagcaccgt ggcgcaaacc gctctcagct atgacgacta ccgtttcctg
    11581 gaaaaactct ctcgcgaagt cggttctcat ttccacgccc tggaccgcac cacgctgtac
    11641 accgccaacc gtgatatcag ctactacacg gtgcatgaat ccttcgttgc cacaattccg
    11701 ctggtgttct gcgaagcgga gaaaatggac cccaataccc agttcctgaa agtgatgatg
    11761 attgatgaac ccgccatcct cgaccaggct atcgcgcgta ttccgcagga agtgaaagag
    11821 aaatataccg tgctgaaaag tgcgccgtac ttcctcgaaa tcctcgataa acgcgttaac
    11881 aaaggtacgg gggtgaaatc actggccgac gtgttaggta ttaaaccgga agaaatcatg
    11941 gcgattggcg atcaggaaaa cgacatcgca atgattgaat atgcaggcgt cggtgtggcg
    12001 atggataacg ctattccttc ggtgaaagaa gtggcgaact ttgtcaccaa atccaacctt
    12061 gaagatggcg tggcgtttgc tattgagaag tatgtgctga attaatcagt ggacgggcaa
    12121 acagcataaa cgcgtttgcc cgcttactga ttattggttt actgcatata caaccagaaa
    12181 ttgcagcggt gcagtaatga tgtccagcgt aacagtcact ggcagtaatg ctgcggaaac
    12241 accatccgga atgcctctgg gtgaatagta ttcataaaat cgcactttaa acggatgata
    12301 gaacgccatc acctttgagt aatccatgtt tttatttttc tcatggattg tgccttttag
    12361 gttttccagt gacctggtac agtgttgaac accacaggca aaaccataat gcgttgccag
    12421 tgccttttct tcttcgttat tccagtggta gtaaagcgat attgtgccgg taaatttctt
    12481 tttccccgca tttaaaacaa atcttgcgtc atcggcaacc tgaatattgt gccggttaag
    12541 tgctggatca ttgagcatct taacgatgtc atcggcacca tcagtgataa ggtaatccag
    12601 cgattcccct acaaaaacat agccttttgt tccattacta tcttttgcct gcgatagccc
    12661 agtgatggtg tctgactgcg attgatactt tcctgaacta ccctttgcgg tatgggaaac
    12721 cagagcatac gagatacagc ctgaaagcat cagcgccagt gggaataaac cggcagcctt
    12781 aaaaaatccc ttaaaattca acttcatgat taatccttta aacgagtagc taagccgttg
    12841 aaagcatact cttctttccc tcctgtttag cgcctgatat cccttttcag atttctgccc
    12901 gacgcatgtc atttttttat gcattgttct ttttgtgatc taaattgtag tacaacaata
    12961 taagtttgta ctacattacg cacacggcaa acgcgaacgt catcacgctg gtactacaaa
    13021 gttgccgcgt tatgcatcga tcggggtaaa gtagagaaga acatacagag cacaaggact
    13081 ctccatgact ctcaataaaa ccgatcgcat tgtcattacg ctgggtaaac agatcgttca
    13141 cggcaaatac gtacctggct cgccacttcc ggctgaggcg gagctctgtg aagagtttgc
    13201 aacctcgcgc aacatcatcc gtgaggtgtt ccgttcgttg atggcgaaac ggctgattga
    13261 aatgaaacgt tatcgcggcg cgtttgtggc accgcgtaac cagtggaatt acctcgacac
    13321 tgacgtactg caatgggtgc tggaaaacga ctacgaccca cggcttatca gtgccatgag
    13381 cgaagtgcga aatctggtgg aaccggcgat tgcccgttgg gcagcagagc gcgcgacttc
    13441 cagcgatctg gcgcagattg aatcggcgct gaacgagatg attgccaaca atcaggaccg
    13501 cgaagcgttt aacgaagcgg atattcgcta ccacgaggcg gtgctgcagt cggtacataa
    13561 cccggtgtta cagcaactta gcattgcgat cagttcgttg cagcgggcgg tttttgaacg
    13621 aacctggatg ggcgatgagg ccaacatgcc gcaaacgctc caggaacata aggcgctgtt
    13681 cgatgcaata cggcatcagg acggcgatgc ggcagagcag gcggcgctta ccatgatcgc
    13741 cagctcgaca cgaaggttaa aggaaatcac atgacagctc gctacatcgc aattgactgg
    13801 ggatcgacca atcttcgcgc ctggctttat cagggcgacc actgcctgga gagcaggcaa
    13861 tcagaagcag gcgtcacgcg cctgaacgga aaatctccgg ctgcggtgtt agcagaagtc
    13921 acgaccgact ggcgtgaaga gaatacgcca gtggtaatgg caggaatggt cggcagcaat
    13981 gtcggctgga aagttgctcc gtatttatct gttcctgccc gtttttcgtc tattggcgaa
    14041 caattaacgt ctgttggcga caatatctgg attattcccg gattatgcgt ctctcatgac
    14101 gataaccaca atgtgatgcg cggcgaagaa acacaattga tcggcgcgcg aactctggct
    14161 ccttcctctc tttatgttat gcccggtacg cattgcaaat gggtgcaggc cgatagccag
    14221 caaatcaacg attttcgcac cgtgatgacc ggtgaattac atcatttact gttaaatcac
    14281 tcattgattg gcgcaggttt gccgccgcag gaaaactctg ccgatgcctt cgcggctggc
    14341 cttgagcgcg gccttaatgc gcccgccata ttgccgcagc tttttgaagt tcgcgcctcg
    14401 catgtgctgg gaacacttcc ccgcgaacag gtcagcgaat ttctctctgg tttgttgatt
    14461 ggcgcagagg ttgccagtat gcgcgactat gtgacccatc aacacgccat cacccttgtc
    14521 gccggaacat cgctgaccgc gcgctaccag caagcctttc aggcgatggg ttgcgacgtg
    14581 acggcggtgg cgggcgacac ggcatttcag gctggtataa ggagcatcgc tcatgcagtg
    14641 gcaaactaaa cttccgctga tcgccatttt gcgcggcatt aagcccgacg aggcgctggc
    14701 gcatgttggc gcggtgattg acgccgggtt cgacgcggtt gaaatcccgc tgaattcccc
    14761 acaatgggag caaagcattc ccgccatcgt tgatgcgtat ggcgacaagg cgttgattgg
    14821 cgcaggtacg gtactgaaac ctgaacaggt cgatgcgctc gccaggatgg gttgtcagct
    14881 catcgttacg cccaatatcc atagtgaagt gatccgccgt gcggtgggct acggcatgac
    14941 cgtctgcccc ggctgcgcga cggcgaccga agcctttacc gcgctcgaag cgggcgcgca
    15001 ggcgctgaaa atatttccgt catcggcttt tggtccgcaa tacatcaaag cgttaaaagc
    15061 ggtattgcca tcggacatcg cagtctttgc cgttggcggc gtgacgccag aaaacctggc
    15121 gcagtggata gacgcaggtt gtgctggggc gggcttaggc agcgatctct atcgcgccgg
    15181 gcagtccgta gaacgcaccg cgcagcaggc agcagcattt gttaaggcgt atcgagaggc
    15241 agtgcaatga aaatcaccaa aattaccacg tatcgtttac ctccccgctg gatgttcctg
    15301 aaaattgaaa ccgatgaagg cgtggtcggt tggggcgagc ccgtgattga aggccgcgcc
    15361 cgtacggtgg aagcggcagt tcacgagctg ggtgactatt tgattggtca ggatccttcg
    15421 cgcatcaatg acttatggca agtgatgtat cgcgccggat tttatcgtgg cggtccaatc
    15481 ctgatgagcg ccattgccgg gatcgaccag gcgttatggg atatcaaagg caaagtgctg
    15541 aatgcgccgg tctggcaact gatgggcggc ctggttcgcg acaaaattaa agcctacagt
    15601 tgggtcggcg gcgatcgtcc ggcggatgtt atcgacggca ttaaaaccct gcgcgaaatc
    15661 ggcttcgata ccttcaaact gaacggttgt gaagaactgg ggctaattga taactcccgc
    15721 gcggtagatg cggcagtcaa caccgtggca caaattcgtg aagcttttgg caatcagatt
    15781 gagtttggtc ttgatttcca cggtcgcgtc agcgcgccaa tggcgaaagt gctgattaaa
    15841 gaactggagc cgtatcgccc gctgtttatt gaagagccgg tgctggcgga acaagccgaa
    15901 tactacccga aattggcggc acaaacgcat attccactgg cggcaggtga gcgcatgttc
    15961 tcacgtttcg attttaaacg cgtgctggag gcaggcggta tttcgattct gcaaccggat
    16021 ctctcccatg caggcggtat taccgaatgc tacaaaattg ctggaatggc agaagcctat
    16081 gatgtgaccc ttgcgccgca ctgtccgctc ggaccgattg cactggcggc ttgcctgcat
    16141 atcgactttg tttcctataa cgcggtactt caggaacaaa gtatgggcat tcattacaac
    16201 aaaggcgcgg agttactcga ctttgtgaaa aacaaagaag acttcagtat ggttggcggc
    16261 ttctttaaac cgttaacgaa accgggctta ggtgtggaaa tcgacgaagc taaagttatt
    16321 gagttcagta aaaatgcccc ggactggcgt aatccgctct ggcgtcatga agataacagc
    16381 gtagcagagt ggtaattcct gccacgtaag cccctcatcg ggcactaaaa cagcaataca
    16441 aaaatataac cctctgtaaa ttacagggca tggtgagcgg cttcgctatg cccaaaatct
    16501 ggagaaagct cgctatggat attcccgtta atgcagcaaa gccggggcgt cggcgttatc
    16561 tgacgctggt gatgatcttt attacggtgg tcatttgtta tgttgaccgc gctaacctgg
    16621 ccgtggcttc cgcccatatt caggaagagt tcggcattac gaaagcggaa atgggctatg
    16681 tattttcggc cttcgcctgg ctttatacgc tatgccagat ccccggcggt tggtttttag
    16741 atcgcgtagg ttctcgcgtg acttatttta ttgcgatatt tggttggtca gtggcgactt
    16801 tattccaggg ctttgccacg ggattaatgt cattaattgg tctgcgtgcg ataaccggta
    16861 ttttcgaagc gcccgctttc ccgaccaata accggatggt gaccagctgg ttcccggaac
    16921 atgaacgcgc ttccgccgtt ggtttttata cgtctggtca gtttgtcggt ctggcgtttt
    16981 tgactccgct gctgatctgg attcaggaga tgttgagctg gcactgggtg ttcattgtca
    17041 ctggtggtat cggcattatc tggtcgctga tttggtttaa ggtttatcag ccgccgcgcc
    17101 tgaccaaagg tatcagcaaa gctgaactgg attacattcg tgatggcggc ggtctggtgg
    17161 atggtgatgc gccggtgaag aaagaggcgc gtcagccgtt aacagccaaa gactggaaac
    17221 tggtgttcca tcgtaaactg atcggcgttt atcttgggca atttgcggtg gcttctacac
    17281 tgtggttttt cttaacctgg ttcccgaact atttaaccca ggaaaaagga atcacggcgc
    17341 tgaaagcagg ctttatgacc acggtgccat tcctcgcggc gtttgtcggc gtcctgctct
    17401 ctggctgggt cgcggatctg ctggtacgta agggcttttc actgggcttt gcgcgtaaaa
    17461 cgccgattat ctgcggcttg ctgatctcca cctgcattat gggcgctaac tacaccaacg
    17521 atccgatgat gattatgtgc ctgatggcgc tggcattctt cggcaacggt tttgcttcga
    17581 ttacctggtc gctggtctct tctctggcac cgatgcgcct gattggttta accggcggcg
    17641 tgtttaactt cgccggtggt ctgggcggca tcaccgttcc gctggtggtg gggtacctgg
    17701 cgcagggtta cggtttcgca cctgcactgg tttatatctc cgccgtcgcg ttgattggcg
    17761 cgctctctta catcctgctg gtgggcgatg tgaagcgcgt tggctaatct ttcaactgtg
    17821 gaatatgcgc cataccactg cgcataatcc gctttcttaa agccggtgcg gtcaggcatc
    17881 ggctttttac tatcttgccg aagagtttta aacgcagttt gcgggtggcg cgccagtaag
    17941 cggtattgag cttctctggc tgcttcagta acaccgaacg cagaatgtct gcgctatcca
    18001 gcgcatagct aatcccctcc agcgaactgg cgctgataaa tcctgccgct tcgccaatca
    18061 gaaaggcgtt gtccttaccg cagacaaaat cctgccagcg tgagggaaac agcaccgtgc
    18121 atttttcgct tttcaccgcc ttaccaaact ggaactgaaa ggcgctcatt ttctctttca
    18181 gcgtcgtgaa acgcgtctga ccgtctttca ttggataagc accgccaaag ataaaataac
    18241 cgtctttgct gatactccat gaataacagt tagttatcga attatcaaag atgcaggagt
    18301 agaacggcac cggatgtttc tctgcgaacc actgctggat agcgacatat ttacggattt
    18361 gatgatccgg gtagagatgt cgccgcacca tcgagtttgc gccatcggca ccgaccagat
    18421 agcgggcagt aatatgttgc tcccagccgt ctgcacgaaa aatgacatgc catttatcat
    18481 cctcacgcca gatttttcga cacaggctgt cgtgataaat ctcaacgctg gcggggatca
    18541 gcgatttcat ccacaagtcg aaagcatggc gattaatatt gatatagctt cgctggtagt
    18601 tacgcgtgag cgatgcggcg acgtcgacag ttttgacgct gaaaatctgc gggttggcga
    18661 tcacatcgac aggaagcgtc agtccatcgc gaataaaaga tcgctgcgcg tccggtgcca
    18721 gcagaccgcc gcaaggtttg ctgaaacctt cagtaccaca ctggtgcttt ttatccagcg
    18781 cgatcacctg cattttgcct gctaattttc gcgccaacgc cgatcctgcc gggccgaggc
    18841 caataatcgc cacgtcgaaa tgttccatct tttcactcac attcatcacg atggcaaaag
    18901 actattgtct gaaagtgaag tccttgtgaa gtatgatggc gggacccgtt ttgtatcagg
    18961 accgcgccat gaaacagata acctttgctc cccgtaatca cctgctcacc aataccaata
    19021 cctggacgcc cgacagccag tggctggtat ttgacgtgcg tccttctggg gcgtcgttta
    19081 ccggcgagac cattgagcgt gtgaatatcc ataccggcga ggtcgaggtt atctatcgcg
    19141 cgtcacaggg cgcacacgtc ggcgtggtga ccgttcatcc gaagtcagag aaatatgttt
    19201 tcatccatgg cccggaaaat cctgatgaaa catggcatta cgatttccat catcgacgcg
    19261 gagtcattgc tgaaggcggc aaggtgagca atctcgatgc aatggatatt accgcgccgt
    19321 atatgccagg agcgctgcgc ggcggcagcc atgtgcatgt ctttagcccg aacggtgaaa
    19381 gggtgagctt tacctataac gaccatgtaa tgcatgaact cgatccggcg ctggatttgc
    19441 gaaacgtcgg tgttgctgcg ccgtttggcc cggtcaacgt acaaaagcag catccgcgtg
    19501 aatacagcgg tagccactgg tgcgtgctgg tgagtaaaac cacgcccacg ccgcagcctg
    19561 gcagcgatga aatcaatcgt gcttatgaag aaggatgggt aggaaatcac gcgctggcgt
    19621 ttattggcga cacactttcg ccaaagggcg agaaagtgcc ggagctgttt atcgttgagt
    19681 taccgcaaga tgaagctggc tggaaagcgg caggtgatgc gccgttaagt ggaacggaaa
    19741 ccaccctgcc cgcgccaccg cgcggcgtcg tgcagcgacg tttaaccttt acccaccatc
    19801 gggcttatcc ggggttagtc aacgtcccgc gccactgggt gcgctgtaat ccgcagggta
    19861 cgcaaatcgc gtttttaatg cgtgatgata acggcattgt gcaactgtgg cttatctcgc
    19921 cacagggcgg cgagccgcgc cagttaaccc ataacaaaac ggatattcag tctgcattta
    19981 actggcatcc gtcaggagaa tggttgggct ttgtgctgga taatcgaatt gcttgtgccc
    20041 atgcgcaaag cggcgaggtt gagtatttaa ccgaaaacca cgccaatcca ccttctgcgg
    20101 acgccgaggt cttctcgccg gatggtcaat ggctggcgtg gatggaaggt ggccagctgt
    20161 ggatcaccga aactgatcgc taaatcagtt atcaggcatt ctggccggtg gaataaccgc
    20221 attggtggct tgcgcgttgg cttcgctttt ttcaacgcgt gagcgcaccg agctgtcttt
    20281 gcggaacaca tcccacggca gtaaaagcgt gtccatcaca gcggtaaacg gcatatcgag
    20341 aatcgccagc gacttagtgc cccagttggt ttcatcgtcg ccaatcatgg ttgcgctggc
    20401 gcgggtgccg ggatacgttc cttctttccc tccggtatgg gacatcacgc tggagcatcc
    20461 tccgagtaat gtcattccgc tgcatatcat gaacgctaac aacacatttc ttatcataat
    20521 aaattcatct gttgatcgtg ggtgttggcc tgatgagtta tagcgatccc ttgctgaaaa
    20581 taacatcatc attacgtcgc actgtggcgg ctatcgcact ttaacgtttc gtgctgcccc
    20641 ctcagtctat gcaatagacc ataaactgca aaaaaaagtc cgctgataag gcttgaaaag
    20701 ttcatttcca gacccatttt tacatcgtag ccgatgagga cgcgcctgat gggtgttctg
    20761 gctacctgac ctgtccattg tggaaggtct tacattctcg ctgatttcag gagctattga
    20821 ttatgcgtaa ctttgattta tccccgcttt accgttctgc tattggattt gaccgtttgt
    20881 ttaaccactt agaaaacaac cagagccaga gtaatggcgg ctaccctccg tataacgttg
    20941 aactggtaga cgaaaaccat taccgcattg ctatcgctgt ggctggtttt gctgagagcg
    21001 aactggaaat taccgcccag gataatctgc tagtggtgaa aggtgctcac gccgacgaac
    21061 aaaaagagcg cacctatctg taccagggca tcgctgaacg caactttgaa cgcaaattcc
    21121 agttagctga gaacattcat gttcgtggcg ctaacctggt aaatggtttg ctgtatatcg
    21181 atctcgaacg cgtgattccg gaagcgaaaa aaccgcgccg tatcgaaatc aactaattcc
    21241 ctaaggccgc ctggcgcggc ctgacatctc catgctcgcc gttagggagc atatgcgaat
    21301 cttcggattt gcaggtactt actcgcttct tagaaggaga aatgactatg cgtaacttcg
    21361 atttatcccc actgatgcgt caatggatcg gttttgacaa actggccaac gcactgcaaa
    21421 acgccggtga aagccagagc ttcccgccgt acaacattga gaaaagcgac gataaccact
    21481 accgcattac ccttgcgctg gcaggtttcc gtcaggaaga tttagagatt caactggaag
    21541 gtacgcgcct gagcgtaaaa ggcacgccgg agcagccaaa agaagagaaa aaatggctgc
    21601 atcaagggct tatgaatcag ccatttagcc tgagctttac gctggctgaa aatatggaag
    21661 tctctggcgc aaccttcgta aacggtttac tgcatattga tttaattcgt aatgagcctg
    21721 aacccatcgc agcgcagcgt atcgctatca gcgaacgtcc cgcgttaaat agctaactag
    21781 ctattctctt tgccccgcca ttctggtggg gctttttttg tcttacttac tcaacaatgc
    21841 gttaaatttc gactgtttaa gatatttcgg cacgttcttt cccgtgataa tgtgcgctat
    21901 aaggcaaatc tgcttcaccg attcagccat aatccgtagc aattaaaaat gttatctcaa
    21961 gagaaatgga cgatgagtga tatagcatta acggtcagta ttctggcttt ggtggcagtc
    22021 gtcggtttgt ttatcggcaa tgtcaaattt cgcggcatag gattaggtat tggcggcgtg
    22081 ctgtttggtg ggatcatcgt cggccatttt gtttctcagg cggggatgac attaagtagc
    22141 gatatgctgc atgttattca ggaatttggc ctgatcctgt tcgtttatac tatcgggatt
    22201 caggtagggc cgggcttctt tgcctcattg cgcgtctccg gattacgcct caacctgttt
    22261 gctgttctga tcgtcatcat cggtggtctg gttaccgcca tcctgcataa actgtttgat
    22321 atgccactgc cggtagtgct ggggattttc tccggtgcgg ttaccaatac gccagcgctg
    22381 ggggcagggc agcagatttt gcgcgacttg ggtacaccaa tggaaatggt cgatcagatg
    22441 gggatgagtt acgcgatggc gtatccattc ggcatttgcg ggattttgtt caccatgtgg
    22501 atgttgcggg ttattttccg cgtcaatgtc gagacagaag ctcagcagca cgagtcttca
    22561 cgcaccaatg gcggcgcgct gatcaagact atcaatattc gcgttgagaa ccctaacctg
    22621 catgatttag ccattaaaga tgtaccgatt ctcaacggcg acaaaattat ctgctcgcgt
    22681 ctgaaacgcg aagaaaccct aaaagttcct tcgccagata ccattatcca actgggcgat
    22741 ttgctgcatc tggtggggca gccagcggat ttacataatg cgcaactggt gattggtcag
    22801 gaggtcgata cttcgctgtc cacgaaaggc actgatttgc gcgtcgagcg tgtggtggtc
    22861 accaatgaaa acgtgctcgg aaaacgtatt cgcgacctgc actttaaaga acgctatgac
    22921 gttgttatct cgcgcctgaa ccgtgccggg gtcgaactgg tcgccagtgg cgatatcagc
    22981 ctgcagttcg gcgatatcct caatctggtg gggcgtccgt ccgcaattga tgccgttgcc
    23041 aatgtgctgg ggaatgcgca gcaaaaactg caacaggttc agatgctgcc agtgtttatt
    23101 ggcatcgggc ttggcgtatt gttaggttct attcccgtct ttgtgcctgg gttcccggcc
    23161 gcgttgaaac tggggctggc gggcggtccg ctgattatgg cgttgatcct cgggcgtatc
    23221 ggcagtatcg gcaagctgta ctggtttatg ccgccaagtg ccaacctcgc gctgcgggag
    23281 ctggggatcg tgctgttcct ctcggtcgtt ggtctgaaat ctggtggaga ttttgtgaat
    23341 accctggtca atggcgaagg gctaagctgg attggttatg gtgccctgat caccgccgtt
    23401 ccgctgatta ctgttggcat tctggcgcgg atgttagcca aaatgaatta cctgaccatg
    23461 tgcgggatgc tggcaggttc catgaccgat cctccggcgc tggcgtttgc taataatctt
    23521 catccaacca gcggtgcggc ggcgctctct tacgccactg tctatccgtt agtgatgttc
    23581 ctgcgcatta tcacccccca attactggcg gtgctcttct ggagtatcgg ttaactcttt
    23641 tccgggtgga gtcgccgtaa atggtactcc acctggtatt cgctggcatt tctgaacatc
    23701 actgaataat tcaaaaactc cccgctctcg ctatatgaca acgaggtgat ccgcagcagt
    23761 ggcgtgtgtt ccgctacctt catttgccgc gccagcagtc tatcggcgag cactggcgtc
    23821 aggctttcat aattaccgcc aatcaaaatc ccgcactctt gttcaatata ttcaaacttc
    23881 gacccttcca gatgttgcag cgaaagatta cggaacagtt ttaccggcat atagctgtct
    23941 tccagcatca gcggtttccc ttccacaaaa cgaacgcgac gggagaagta gatctgctcg
    24001 ttgatttgaa tccgtaactg gctggcaatg gccggaggcg caggcatgat ttcaaaaatc
    24061 agcacctggc tggtgaccgt ttttccctgc cgttttaaca cctccaccag cccggtcagg
    24121 ctggcggttt gatgcagtac atctttgcgc accaggtaag tgccgctgcc gtggcggcgg
    24181 accaccaatc cccacgctac cagcaggtca atggctttac ggatggtcat ccgcgatacc
    24241 gcaaattctt ccgccaactt tttttcaccg ggaagaaggc tatttagcgt gaaatctgcg
    24301 gaattaagtc gaattcttaa ccgctccgca atgcttttgt agatcatctc tagatctctt
    24361 ttttgcctgc aagcacgggt aatgcgccca caagttctgc ttaatcgatt gaaaaaacat
    24421 ctttttttta aagatgtgtt cgatgctgtg tacctgtgct cacagatgtc tactttttcg
    24481 cgaaaacgta gatctctacc gcccaacgaa aagcatgaaa gcgatcacga atcccattgg
    24541 gtcgtcatgt tctgccgatc gcatcttcct atcctcgctc caggcctgcc gcataaccaa
    24601 tcaggcttcc tacttacaga attgagaaaa gaggatgtgg aaatgctcag tcaaattcaa
    24661 cgctttggcg gcgcgatgtt cacgccagtg ctgctgtttc ccttcgccgg gattgtggtg
    24721 ggtcttgcca tcttgctgca aaacccgatg tttgtcgggg aatcactgac cgatccgaac
    24781 agtttattcg cgcaaatcgt acacattatt gaagagggcg gttggacggt gttccgtaat
    24841 atgccgctga tttttgctgt cggtttaccc attggccttg ctaagcaagc gcaggggcgt
    24901 gcttgtctgg cggtgatggt gagtttcctg acctggaact atttcatcaa cgcgatggga
    24961 atgacctggg gaagctactt cggcgtcgat ttcactcagg acgcggtggc aggtagcggt
    25021 ctgacaatga tggccgggat taaaaccctc gataccagca ttatcggcgc aattatcatt
    25081 tccggcattg tgacggcgct gcataaccgt ctgttcgata aaaaactgcc ggtttttctc
    25141 ggcattttcc aggggacgtc ttatgtggtg attatcgcct tcctggtgat gatcccctgt
    25201 gcctggctga cgttgctcgg ctggccaaaa gtacaaatgg ggattgaatc tctgcaagcg
    25261 ttcctgcgtt cggcgggtgc gcttggggtg tgggtttaca ccttcctcga acgtattctg
    25321 atcccaaccg gtttacacca cttcatctac ggaccgttta tctttggtcc ggcagctgtt
    25381 gaaggcggca ttcagatgta ctgggcgcag catctgcaag agttcagtct gagcgccgag
    25441 ccgctgaaat cgttgttccc ggaaggcggt tttgccctgc acggtaactc aaaaatcttt
    25501 ggtgccgtgg gcatttcttt agcgatgtac ttcactgccg caccggaaaa tcgggtaaaa
    25561 gtggcgggct tgctgattcc cgcaacctta accgccatgc tggtgggaat taccgaaccg
    25621 ctggaattta ccttcctgtt catttcaccg ttgctgtttg cggtacacgc cgtgctggcg
    25681 gcctcaatgt cgaccgtgat gtatctcttt ggtgtggtgg gcaacatggg cggaggtctg
    25741 attgaccagg ttttaccgca aaactggatc ccgatgttca gcaaccacgc ggatatgatg
    25801 ctgacccaaa tcgccattgg gttgtgcttt accctgctgt acttcgtggt tttccgcacc
    25861 ctgattctgc agttcaacat gtgcacgccg ggacgtgaag atgcggaagt gaaactctac
    25921 tcaaaagccg aatacaaagc ctcgcgaggc caaaccaccg cggcagagcc aaaaaaagag
    25981 ctggatcagg ctgccggtat tctgcaagcc ctgggcgggg tcggcaatat ctccagcatt
    26041 aacaattgcg cgacgcgtct acgcattgca ctgcatgaca tgtcacaaac gctggatgac
    26101 gaagtcttta aaaagctggg agcgcacggc gtcttccgta gtggcgatgc cattcaggtg
    26161 atcattggtc tgcatgtatc ccaattgcgt gaacagctcg atagcttaat taattctcat
    26221 caatcagcag aaaatgttgc cattacggag gcagtataat gaccaaattc tcagtggttg
    26281 tcgcaggcgg tggaagcacc tttacgccag gcatcgtgtt gatgctcctg gcgaatcagg
    26341 accgtttccc gcttcgtgca ctgaaatttt atgataacga tggcgcgcgg caggaagtga
    26401 ttgccgaagc ctgtaaagtc atccttaaag aaaaagcgcc ggacattgcg tttagttaca
    26461 ccaccgatcc tgaagtagcg ttcagcgacg ttgattttgt catggcgcac atccgcgtcg
    26521 gcaagtaccc gatgcgcgaa ctggatgaaa aaatcccact gcgccacggt gttgttggtc
    26581 aggaaacttg cggacccggc ggaatagcgt acggcatgcg ttccattggc ggcgtcctgg
    26641 aactggtgga ttatatggaa aaatattcac caaacgcctg gatgctcaac tactccaacc
    26701 cggcagcaat tgtcgcagaa gccacgcgtc gtctgcgccc gaatgcgaaa atcctcaaca
    26761 tctgtgacat gccaatcggt attgaaagcc ggatggcgca aattgttggg ctgcaagatc
    26821 gcaaacagat gcgcgtgcgc tactacggcc tgaaccactg gtggtcggcg atatcccgca
    26881 gtttcagaaa gggttaatga gtcagcaagt ggcggtggaa aaactggtcg tggacgcctg
    26941 ggaacagcgt tcatatcagc acctgtggca ggcgattacg ttgtcgaaaa cggtaccgag
    27001 cgcctcggtc gccaaagcta ttctggatga gttgctggag gccaacaaag cgtactggcc
    27061 agagttacgt taattgtgtg gactggcatc cgttgggtgc tggtccgtat tctttagcac
    27121 ggctgacggc gcataaccaa atttatgttt aaaggctttg ctgaaatgaa aggagtcgaa
    27181 gaagttgagc gtatcagcga cttcgccaac cgagtttcct tgttgcttta atagcgatag
    27241 cgccagatcc agacgcgcat cgaggtaata ttctttcggc gtcttgccgg tatagcgtaa
    27301 aaacagacga cgcaaccagg cttcgctaca ggggatcgtg gcagccatat cagctacgct
    27361 ccagcgttgt tgcagactgg catgtaaagt ggcaattaat ttttcaattt gccgccgttg
    27421 tggatctttt ttaccatctg cgtacataag acaaatccac tgataaataa ttttcgtcag
    27481 aaaagcgact gccagattat tttttattgc ttctggtgaa gttattaact cagcaacttc
    27541 ggtgagttcc tgattataaa tttcgccatt ataaataacg ctttgctgac caacgggaat
    27601 atccatcata ctggtggggg taaattccat ccagtactgt tcccagacta aaccttcaca
    27661 gtgataagag tgaatatcca ttggctttaa gaatataata cagttgccat gcagtgttat
    27721 ttgttcgcca ttctttaaaa gaattttccc acatccttgt acggtataca ccgcaaccca
    27781 tgagttggcg ataagcggtt tcttcttgtc ccgcggccag atcacccggt aatgctcgtc
    27841 ggcgtaggta tgccagagcg agatcagaga aattccgctg gagatcacat tttcgttaat
    27901 caataaggga atattgtacg gagtttcgtt ttttacatct gaactttgca attttccatt
    27961 cattttaata tccctccggc gaattatgct cacaacctga acttaacaga aaacaatccg
    28021 ttcagggata cgacgtttgt attttaagaa tctgactgcc tgacccgacg cattttacct
    28081 ctccctatat tcatgcgtcc gggacataaa cataaataag ggctatgaga tgaattcgtt
    28141 acaaatcttg agttttgtcg gttttacgct gctggtggcg gtgatcacct ggtggaaggt
    28201 ccgcaaaaca gataccggat cgcaacaagg ctattttctt gccggacgtt cactaaaagc
    28261 gccggttatt gccgcttcgt taatgctaac caacctttcc acggaacaac tggtcggcct
    28321 ttccgggcag gcctacaaaa gcggcatgtc ggtgatgggc tgggaagtga cttcagcggt
    28381 gacgctgatc ttcctcgcgc taatcttttt accgcgctat ctgaagcgcg gcattgccac
    28441 catccccgat tttctggagg aacgttatga taaaacgacg cgtattatca tcgacttctg
    28501 cttcctcatt gccaccggcg tctgctttct gccgattgtt ctctactccg gcgcgttggc
    28561 gctcaacagc ctgtttcacg tcggggaatc gctacagatt tctcacggtg cggctatctg
    28621 gctactggta attttgcttg gtctggcggg aattttgtat gcggtgatcg gcggactgcg
    28681 cgcaatggca gtggcggact ccatcaacgg tattgggctg gttatcggcg ggttgatggt
    28741 gccggtattt ggcctgatcg cgatgggcaa gggcagcttt atgcaaggca ttgagcaact
    28801 caccaccgtt cacgccgaga aattaaactc aatcggtggc ccgaccgatc ccttgccgat
    28861 tggcgcggca tttaccggtt tgattctggt gaacaccttt tactggtgta caaatcaggg
    28921 catcgtgcaa cgcacgctgg cgtcaaaaag cctggcggaa gggcaaaagg gggcgctgtt
    28981 aacggcggtg ctgaaaatgc tcgacccgct ggtactggtg ctgccagggt tgattgcgtt
    29041 tcatctgtat caggatttac cgaaagccga catggcctac ccgacgctgg tcaataacgt
    29101 tctgccagtg ccaatggtgg gtttcttcgg cgcggtgtta tttggtgcgg tgatcagtac
    29161 cttcaacggc tttctgaata gcgccagtac gttattcagt atgggtattt accgtcgcat
    29221 cattaaccag aatgccgagc cgcagcagct ggtcaccgtc gggcgcaaat ttggtttctt
    29281 tatcgctatc gtttcggtgc tggtcgcgcc gtggatcgcc aacgcgccgc aggggctgta
    29341 tagctggatg aaacagctca acggcattta caacgtgccg ctggttacca tcatcattat
    29401 gggctttttc ttcccgcgca tcccggcgct ggcggcaaaa gtggcgatgg ggattggcat
    29461 aatcagctac atcaccatca actatctggt gaagttcgac ttccatttcc tctatgtgct
    29521 ggcctgtacg ttctgcatca acgtggtcgt gatgctggtg atcggtttta tcaaaccgcg
    29581 cgccacgccg ttcaccttca aagatgcgtt tgcggtggac atgaaaccgt ggagaaacgt
    29641 caagatcgcg tcaattggca tcctgttcgc gatgattggc gtctatgccg ggctggctga
    29701 attcggcggc tacggtacgc gctggttagc gatgatcagt tatttcattg ccgccgtagt
    29761 gattgtctac ctgatttttg acagctggcg gcatcgtcac gacccagccg taacctttac
    29821 tcccgacgcg aaggatagcc tatgaaacgc cccaattttc tgttcatcat gaccgatacc
    29881 caggccacca atatggtcgg ttgctatagc ggtaaaccgc tgaatacgca aaatattgat
    29941 agtctggcgg cggaaggtat tcgctttaat tccgcctaca cctgttcacc ggtttgtacg
    30001 cctgcacgcg ccggactatt taccggtatc tacgctaacc agtccggccc gtggaccaac
    30061 aacgtcgcgc caggcaaaaa catctccact atggggcgct actttaagga tgccggctat
    30121 cacacctgtt acatcggcaa atggcatctc gacggtcatg actatttcgg cactggcgag
    30181 tgtccgccgg agtgggacgc tgattactgg ttcgatgggg cgaactacct tagcgaactg
    30241 acggaaaaag agatcagcct gtggcgcaat ggcctaaaca gcgttgagga tttacaggcg
    30301 aaccatatcg acgaaacctt cacctgggcg caccgcatca gcaatcgggc agtagatttt
    30361 ctgcaacagc ccgcgcgcgc cgaggaaccc ttcctgatgg tggtttcgta tgatgagccg
    30421 catcacccgt tcacctgtcc ggtggagtat ttagagaaat acgctgattt ttactacgat
    30481 ctgggcgaga aagctcagga tggcctggcg aacaaaccgg aacatcaccg cttatgggcg
    30541 caggcgatgc catcgccagt cggtgatgac gggctttatc accatccgct ctattttgcc
    30601 tgtaatgact ttgttgatga ccaaatcgga cgggtcatca atgccttaac gccagagcaa
    30661 cgtgaaaata cgtgggtcat ttatacttcc gatcacggcg aaatgatggg cgcacataag
    30721 ctgatcagta aaggagcggc gatgtatgac gacatcaccc gtattccgct gatcatccgt
    30781 tcgccgcaag gggagcggcg acaggtcgat acgccagtca gtcatatcga tttactgccg
    30841 acaatgatgg cgctggcaga tattgaaaaa ccagagattc tgccggggga aaatatcctt
    30901 gccgtgaaag agccacgcgg tgtaatggtg gaatttaacc gctacgagat tgagcatgac
    30961 agctttggcg gttttattcc ggtgcgttgc tgggtgacgg atgactttaa actggtactc
    31021 aacctcttca ccagtgatga actttacgat cgccgtaatg acccaaatga aatgcataac
    31081 ctgatcgatg atatccgttt tgcagacgtt cgcagcaaaa tgcatgacgc cttattggat
    31141 tacatggaca aaattcgcga tccgttccgc agttaccaat ggagcctgcg tccgtggcgt
    31201 aaagatgcac tgccgcgctg gatgggggca tttcgtccac gcccacaaga tggctattcg
    31261 ccggtggtac gtgactatga caccggccta ccgacgcaag gagtgaaagt ggaagagaaa
    31321 aaacagaagt tctgacgata atgcccgctg atgaacaccc ggcgggcatt attcaggctg
    31381 cttaaaccag tgaaccgccg gtcaggaagg aaaaaatcag gaatatcagc aaaatgctgc
    31441 acacgatgcc gataccaaaa agcaaggtat ggaaagccag cgtcccgccg ccaaaaacca
    31501 gcgcgccgcg aatgccccag cgattttcct tacgcatacc gccaataaat gcgcaaagta
    31561 gcgcagcaat ggccagtgcg ataccgcttt gatcgaggat tttatcgaca tctacgtcct
    31621 cttttttctc cacggtggta attttttcgc cttttattcc ggcgagaaga cctcttttaa
    31681 tttcagccgt tttgtccgcc acgatgcttt caaacgttgg cggtggtgcg gaaaaagggc
    31741 cgaatgaaaa gtggataatc cccatcatta aagcgatggc accgaacagc atgcccaatg
    31801 aactgatttt attttgagct atgataccca tctgacctcc ttgtagcgga tggcgaaagc
    31861 atatcaggaa acgggcggac gaaattctta aaaaaaaacc gctaaaggta gagcgttagt
    31921 gaaattgcaa aggagcaaca atgaaaattt cccgccttgg agaagcgccc gattaccgct
    31981 tctcgctggc taatgagcgt acttttctgg cgtggatccg taccgcacta ggatttctgg
    32041 cggcgggggt tgggcttgat caacttgcgc cagatttcgc cacgcccgtc attcgtgaac
    32101 tgctggcgtt gttgttgtgt ctgttttccg gcgggttagc gatgtatggc tacctgcgct
    32161 ggttacgcaa tgaaaaagcg atgcgtctga aagaggacct gccctacacc aacagcttat
    32221 taatcattag cttaatattg atggtcgtgg cggtgatcgt tatgggactg gtgttgtatg
    32281 ccggatagcc gcaaagccag acgcattgcc gacccaggac tgcagccgga gcgcacatca
    32341 ctggcgtggt ttcgtaccat gctgggctac ggcgcgttga tggcgttggc tatcaaacac
    32401 aactggcacc aggcgggcat gttattctgg atttcgattg gcattctcgc catcgtggcg
    32461 ctgatcctct ggcactacac ccgtaatcgc aatttaatgg atgtcacgaa tagcgatttt
    32521 tcccaatttc acgtagtccg tgacaaattt ttgatctccc tcgcggtgtt atctctcgca
    32581 atactgtttg ctgtaacgca tatacatcaa cttatcgtat ttattgagag agtcgcatga
    32641 caggaagtca ggtcattgat gctgaagagg acaggcataa actggttgta gagtacaagg
    32701 atgctctgca acctgccgat ttttatcaca atttcaaaca gcggggcatc cgctccgtac
    32761 agctcattcc ctatcttgaa tttgatgacc ggggcgatct gacggccgct tcggtgacag
    32821 cagagctgtg ggggaaattt ttaattgccc tgttcgagtg ttgggtgcgg gcagatatca
    32881 gtcgtatctc gattgagctt tttgacgcca ccttacaaaa atggtgtggc agcgaaaacc
    32941 cgcaaccgcg ccgtgactgc caggcgtgcg actggcatcg cctgtgcccg cacgctcgcc
    33001 aggagacgcc agacagcgtg ctttgtgcgg gctatcaggc gttttattct tactccgcac
    33061 cgcatatgcg ggtgatgcgt gatctgataa agcagcatcg ttctcccatg gagctgatga
    33121 cgatgctgcg gtgacgtgcg cttaaacggg ctgcccctga tgcgacatcc gcgtcgccag
    33181 cggcagccag cacagcacga tcaacaatcc cattaaggtc atcaacaacc ccaggctgcc
    33241 ctgaccggtt tgcggcaaca tcgcagagag cgacgccagc acgccggaac caatgttttg
    33301 cagaccgccg accagcgcgc cagccgtgcc cgccaggaag gggaacggct ccatcgcgcc
    33361 gctggtcgcc agcggaaaca gcatcccggc accgaaaaag aacagcgcgg cgggaacgag
    33421 cagcgtccag acattcatca cgccaaacca gtcggggatc cacatcagca agccagccag
    33481 caggcagcag ataaccgact gccacattaa cgtggagaag cgtttattgg gacgtccggc
    33541 aaaccatgcg ccaaaaaacg ctgccggaat cggcagaata aacaaaatac tgaccgtcat
    33601 actgctcagc cctaacaccg cgcccatcag cacgccggag caggcttcaa aggcggcaat
    33661 cccggccaga ccgccaatca gcatcagcaa ataacagtta aaaccgctgt taccgaaaag
    33721 cgttttataa ctggtaagca ggcgcgtgcg cggtgcatcg accggacgcg tttccggcat
    33781 ccagcgggcc atactgaagg tcacaccagc acaaagaacc aacaaaaaga gataacaggc
    33841 gcgccagttc cacatcgtat ccagcagacc gccgattagc ggtgcgagca acggactgac
    33901 gagaatcccc atgtttaaca ggctgttagc atggcgcaac tgtgtccgtt catataaatc
    33961 tcgcggcaaa gtacgcgcca ttacgccgcc aacgccggtg cccatcccct gcatcgcgct
    34021 ggcggcaatc aacaccgtca aactggaggt cgtgaccgcg accagcgttg ccagcataaa
    34081 aatggacatt ccgacgagga tcaccggtcg gcggcccacg cggtcggaaa tcgggccata
    34141 aaacagctgt gagacaccgt aagtcagcag ataagcgccc attacgctct gcaccgcccc
    34201 ttcacggacg ttgagatcgc gcgccatatc ggcaatagct ggaatataaa tggtttgcgc
    34261 catctgaccg acggccacga gtaataccaa catcaataac aaattgacgt ttctttgcct
    34321 tttcattatc acggatgctt ttataaaaat aattagaata agtcactacg cattcccata
    34381 aatgcgggag aggaatctat cacattgatg aatgttagcc agatatacgc caggaatggg
    34441 gaattgttta gcggcagaat atgtaaacaa aagcggcaat aaatgttgcc gggagggatt
    34501 acgtgcgaac tgtaaccctc tccccaaagg ggcgaggggg aaaccaccgt tccctttcag
    34561 aaaatgagct aaacattgca cgggacggtc ccctcgcccc tttggggaga gggctagggt
    34621 gaggggcaca ggcagcgcag gtgcaaacat tctgctggta gaagaagccc ccaactccaa
    34681 cccgcacgct aaatataaaa ggggagcggt tacccgctcc cctttggtgc gacttgaatc
    34741 tgaattactt caggtatttc agaacagcat caagcagttg cagtgctgca acaatgagtt
    34801 tgaggataag aatggcgata tccaccaggc tcatacgcgt ctcctgtggt tcaggagcac
    34861 tgccagctga cgtacctttc cgctgcctgt tgccagctgt tgtgcacgcg gtatcttaag
    34921 gagaaacgtt gtgcttgttg acataacaca gtgtgctcac gcggggttaa ggcgctgacg
    34981 gcaccacccg tttcagccag gacttagtgc gccgggtaac gatgcggccc ccgcataaca
    35041 cattgcgtac agtgatatta tagaagttta ctgtataaat aaacagtaat atttggacaa
    35101 aacgcaaact gtgatcgata ctcaactatg ccctaaatac gtcaaaattc gtgccgaaat
    35161 tgcgcgttct gcgcggaaca cgtatacttt cagtgttgac ataatacagt gtgctttgcg
    35221 gttaccagcc gcaggcggct gatgaaacct cgctccggcg gggttttttg ttacctgcaa
    35281 ttcagtacaa aacgtgatca gcccctcaat tttcccttgc tgaaaaattt tccattgtct
    35341 cccctgtaaa gctgtgcttg tataaatatt gttaaacaca aaaccaacaa ggtccccaat
    35401 gactacttcc atgttcaacg caaaactact accaactgcg ctatccgccg cagtggtcgt
    35461 cgtgcgtgtg gtggtggtcg tcggcaatgc gccgtaggga ctggaacaac acacgattcc
    35521 aaaaccccgc cggcgcaaac cgggcggggt ttttcgttta agcacctccc ggaaagtcgg
    35581 cccagaagaa aaggactgga gcatggcaag ttcgggcaca acatcgacgc gtaagcgctt
    35641 taccggcgca gaatttatcg ttcatttcct ggaacagcag ggcattaaga ttgtgacggg
    35701 cattccgggc ggttctatcc tgcctgttta cgatgcctta agccaaagca cgcaaatccg
    35761 ccatattctg gcccgtcatg aacagggcgc gggatttatc gctcagggga tggcgcgcac
    35821 cgacggtaaa ccggcggtct gtatggcctg tagcggaccg ggtgcgacta acctggtgac
    35881 cgccattgcc gatgcgcggc tggactccat cccgctgatt tgcatcaccg gtcaggttcc
    35941 cgcctcgatg atcggcaccg acgtcttcca ggaagtggac acctacggca tctctatccc
    36001 catcaccaaa cacaactatc tggtcagaca tatcgaagaa ctcccgcagg tcatgagcga
    36061 tgccttccgc attgcgcaat caggccgccc aggcccggtg tggatagaca ttcctaagga
    36121 tgtgcaaacg gcagtttttg agattgaaac acagcccgct atggcagaaa aagccgccgc
    36181 ccccgccttt agcgaagaaa gcattcgtga cgcagcggcg atgattaacg ctgccaaacg
    36241 cccggtgctt tatctgggcg gcggtgtgat caatgcgccc gcacgggtgc gtgaactggc
    36301 ggagaaagcg caactgccta ccaccatgac tttaatggcg ctgggcatgt tgccaaaagc
    36361 gcatccgttg tcgctgggta tgctggggat gcacggcgtg cgcagcacca actatatttt
    36421 gcaggaggcg gatttgttga tagtgctcgg tgcgcgtttt gatgaccggg cgattggcaa
    36481 aaccgagcag ttctgtccga atgccaaaat tattcatgtc gatatcgatc gcgcagagct
    36541 gggtaaaatc aagcagccgc atgtggcgat tcaggcggat gttgatgacg tgctggcgca
    36601 attgatcccg caggtggaag cgcaaccgcg tgcagagtgg caccagttgg tagcggattt
    36661 gcagcgcgaa ttcccgtgtc caatcccgaa agcgtgcgat ccattaagcc attacggcct
    36721 gatcaacgcc gttgccgcct gtgtcgatga caatgcgatt atcaccaccg atgtgggtca
    36781 gcatcagatg tggaccgcgc aagcttatcc gctcaatcgc ccacgccagt ggctgacctc
    36841 cggtgggctg ggcacgatgg gttttggcct gcctgcggcg attggcgcgg cgctggcgaa
    36901 cccggatcgc aaagtgttgt gtttctccgg cgacggcagc ctgatgatga atattcagga
    36961 gatggcgacc gccagtgaaa atcagctgga tgtcaaaatc attctgatga acaacgaagc
    37021 gctggggctg gtgcatcagc aacagagtct gttctacgag caaggcgttt ttgccgccac
    37081 ctatccggga aaaatcaact ttatgcagat tgccgccgga ttcggcctcg aaacctgtga
    37141 tttgaataac gaagccgatc cgcaggctgc attgcaggaa atcatcaatc gccctggccc
    37201 ggcgctgatc catgtgcgca ttgatgccga agaaaaagtt tacccgatgg tgccgccagg
    37261 tgcggcgaat actgaaatgg tgggggaata agccatgcaa aacacaactc atgacaacgt
    37321 gattctggag ctcaccgttc gcaaccatcc gggcgtaatg acccacgttt gtggcctttt
    37381 tgcccgccgc gcttttaacg ttgaaggcat tctttgtctg ccgattcagg acagcgacaa
    37441 aagccatatc tggctactgg tcaatgacga ccagcgtctg gagcagatga taagccaaat
    37501 cgataagctg gaagatgtgg tgaaagtgca gcgtaatcag tccgatccga ctatgtttaa
    37561 caagattgcg gtgttttttc agtaaccgct caaggcttga acaacatcgc gcttatcgtt
    37621 aaggtaagcg cgtttttttt tacccgccag gacaagacca tgatcaccgt tgcccttata
    37681 gacgatcacc tcatcgtccg ctccggcttt gcgcagctgc tggggctgga acctgatttg
    37741 caggtagttg ccgagtttgg ttcggggcgc gaggcgctgg cggggctgcc ggggcgcggt
    37801 gtgcaggtgt gtatttgcga tatctccatg cccgatatct ccggtctgga actgctaagc
    37861 cagctgccga aaggtatggc gacaataatg ctctctgttc atgacagtcc ggcgctggtt
    37921 gagcaggcgc ttaacgcggg ggcacgcggc tttctctcca aacgctgtag ccctgacgaa
    37981 ctgattgcgg cggtgcatac ggttgccacg ggcggctgtt atctgacgcc ggatattgcc
    38041 attaaactgg catccggtcg tcaggacccg ctaaccaaac gcgaacggca ggtggcggaa
    38101 aaactggcgc aaggaatggc ggtgaaagag attgctgccg aactgggctt gtcgccgaaa
    38161 acggtgcacg tccatcgcgc caacctgatg gaaaaactgg gtgtcagtaa cgacgtagag
    38221 ctggcgcgcc gcatgtttga tggctggtga tgaagacgtt gttctcccgc ttaattaccg
    38281 ttattgcctg cttttttatc ttctctgccg catggttttg cctgtggagt atcagcctgc
    38341 acctggttga gcgccctgat atggcggtgc tgttatttcc gtttggtctg cgtctggggc
    38401 taatgctgca atgcccgcgc ggatactggc cggtgctgct gggcgcggag tggctgctga
    38461 tttactggct aacgcaggcg gtcggtttaa cccattttcc gttattgatg atcggtagtt
    38521 tactgacgtt actgcccgtg gcgctgatct cgcgctatcg ccatcagcgt gactggcgca
    38581 ccttgctgtt acagggtgcg gcgttaacgg cggcggcgtt gttgcagtcg ctgccctggc
    38641 tttggcacgg caaagagtcg tggaatgcgc tgttgctgac tttaactggc ggcctgacgc
    38701 tggccccgat atgtctggtg ttctggcact atctcgccaa taacacctgg ctgccgctcg
    38761 gcccgtcact ggtttctcag ccgatcaact ggcgcgggcg acatctggtc tggtacttgc
    38821 tgctgtttgt tatcagtctc tggctccagt tgggattgcc ggacgaactg tcgcgcttta
    38881 cgccattttg tctggcgctg ccgattatcg cgctggcctg gcactatggt tggcaagggg
    38941 cgctgattgc gacgttgatg aacgccatcg cgctgatcgc cagtcaaacc tggcgcgatc
    39001 atccggtgga tttattgctc tcgctgctgg tgcaaagtct gacagggttg ttgcttggcg
    39061 ctggcatcca gcggttgcgt gaacttaacc agtcgctgca aaaggaactg gcgcgcaatc
    39121 agcatctggc tgaacggttg ctggaaaccg aagagagcgt gcgccgtgat gtggcgcgtg
    39181 agctgcatga tgatatcggt cagaccatca cagccattcg tactcaggcg ggcattgttc
    39241 agcggctggc ggcagataac gccagcgtga agcagagcgg gcagctcatc gaacaactat
    39301 cgctgggcgt ttacgacgcg gtgcgccgtt tgttgggtcg gttacgtccg cgccagttgg
    39361 atgatctcac cctggagcag gccatccgct cactgatgcg ggaaatggag ctggaagggc
    39421 gcggtattgt cagccatctc gaatggcgaa tcgatgaatc agcgttaagc gaaaaccagc
    39481 gcgtgacgct gtttcgtgtc tgccaggaag ggctgaacaa cattgtgaaa catgctgatg
    39541 ccagcgcggt caccctgcaa ggctggcagc aggatgaacg gttgatgctg gttattgaag
    39601 acgatggcag cggtttgccg ccgggttccg ggcaacaagg ttttggcctc accggaatgc
    39661 gcgagcgcgt aacggcgctg ggtggcacat tacacatttc ctgtctgcac ggcacgcgtg
    39721 tcagcgtttc tctacctcaa cgctatgtct aaggtttgat gatgttgccg tttctgaaag
    39781 cgcctgccga tgcgccatta atgactgata aatatgaaat tgatgcccgc tatcgctact
    39841 ggcgtcggca tattctgctg accatctggc tgggttacgc gctgttttac ttcacgcgga
    39901 aaagttttaa cgccgcggta ccagaaatcc ttgctaacgg cgtgctcagc cgtagcgata
    39961 tcggcctgtt agcgaccctg ttttacatta cttacggcgt gtcgaagttt gtctccggca
    40021 ttgtcagcga tcgctcaaat gcccgttatt ttatggggat agggcttatc gccacgggca
    40081 tcatgaacat tctgtttggc ttctcgacgt cgctatgggc gtttgccgtg ctctgggtgc
    40141 tgaacgcctt tttccagggc tggggttcac cggtgtgtgc gcgtctgtta acggcctggt
    40201 attcacgtac cgagcgcggc ggttggtggg cattatggaa cacggcgcat aacgtcggcg
    40261 gcgcactcat tcccattgtg atggcagcga gcgcgctgca ttacggctgg cgtgccggga
    40321 tgatgattgc tggttgtatg gcgatagtcg tggggatttt tctctgctgg cggctacgcg
    40381 atcgcccgca ggcgttaggt ttaccggcgg tcggtgaatg gcggcacgac gcgctggaaa
    40441 ttgctcaaca acaagaaggg gcagggttga cgcgtaaaga gatcctgacc aaatatgtgt
    40501 tgctgaatcc gtatatctgg ctgctttcgt tttgctatgt gctggtctat gtggtccggg
    40561 cggcaatcaa cgactggggc aatttgtata tgtccgagac gctgggcgtc gatctggtca
    40621 cggcgaatac ggcagtgacg atgtttgaac tgggcggatt tatcggtgcg ctggtagccg
    40681 gttggggctc ggacaaattg tttaacggca accgtgggcc gatgaatttg attttcgccg
    40741 ccggaatttt gctttcagtc ggctcgctgt ggctgatgcc atttgccagc tacgtgatgc
    40801 aggcaacctg cttcttcacc attggttttt ttgtctttgg cccgcagatg ttaatcggca
    40861 tggcggcggc agagtgttcc cacaaagagg ctgcaggggc ggcgacgggg tttgtcggct
    40921 tgtttgctta tctgggggcg tcgcttgctg gttggccgct ggcgaaagta ctcgatacct
    40981 ggcactggag cggatttttt gtggttatct ctatcgccgc cggggtttcc gcactgctgt
    41041 tactgccctt tttgaacgcc caggcaccgc gcgaagcgtg atgcatctca ccttttcact
    41101 tcatatccgg caaaactaag aaattttcca ggttttgcct ggacgctatc tcaggcctga
    41161 tttgctgctg atttttacaa tgccatcatt cattaattct ttcccaaaat aattcaagtt
    41221 gcaggaacgc agcaaacgcg tgagtcccgg gagcatagtg aactatgtga ccggaaggaa
    41281 tgcgtgcagc taaggcacct gcggcttgaa gtatgaaggg aaatcaggag taacccatgc
    41341 tggctttctt aaaccaggtt cgcaagccga ccctggacct tccgctcgaa gtgcggcgca
    41401 aaatgtggtt caaaccgttc atgcaatcct acctggtggt ctttatcggc tacctgacga
    41461 tgtacctgat tcgcaagaac tttaacatcg cgcagaacga catgatctcg acctacgggt
    41521 tgagcatgac gcagctgggg atgatcggcc tgggtttctc catcacttat ggcgtgggta
    41581 aaacgctggt ttcctactac gccgacggca aaaacaccaa acaattcctg ccgttcatgc
    41641 tgatcctgtc tgccatttgt atgctgggct tcagtgccag tatgggcagc ggctcggtta
    41701 gcctgttcct gatgattgcc ttctacgcct taagcggctt tttccagagt accggcggtt
    41761 cgtgcagtta ctccaccatc accaaatgga cgccgcgtcg taaacgcggg acattcctcg
    41821 gtttctggaa tatttctcac aaccttggcg gtgcaggcgc agcaggtgtg gcgctgttcg
    41881 gggcaaatta cctgttcgat ggccatgtca tcggcatgtt tatcttcccg tcgattatcg
    41941 cgctgattgt cggttttatc ggcctgcgtt acggcagcga ctccccggaa tcttatggcc
    42001 tcggcaaagc tgaagaactg ttcggcgagg agatcagcga agaggacaaa gagacagaat
    42061 ctaccgatat gaccaagtgg cagatctttg ttgagtatgt gctgaaaaac aaagtgatct
    42121 ggctgctgtg cttcgccaac attttcctct atgtggtacg tattggtatc gaccagtggt
    42181 caaccgtata cgcgttccag gaactgaaac tctctaaagc ggtggcgatt cagggcttta
    42241 cgctgtttga agctggtgca ctggtcggta cgctgctgtg gggctggctc tctgacctgg
    42301 cgaacggtcg ccgtggcctg gtggcctgca tcgcgctggc gctgattatc gccacgctcg
    42361 gtgtgtatca acatgccagc aacgaatata tctatctggc ttctctcttt gcgttgggtt
    42421 tcctggtctt tggcccgcaa ttgttgattg gtgtggctgc tgttggcttt gtacctaaaa
    42481 aagcgattgg cgctgccgat ggtattaaag gcacctttgc ttacctgatt ggtgacagct
    42541 ttgccaagtt aggtctggga atgattgccg atgggacgcc ggtattcggc cttaccggct
    42601 gggcaggcac cttcgccgca cttgatatcg ccgcgattgg ttgtatctgc ctgatggcga
    42661 tagtggcggt gatggaagaa cgcaaaatcc gccgcgagaa aaaaattcag cagttgacag
    42721 tggcataaac gtaactggtg acttttgccc ggcatgacgc cgggcttttt tattattccg
    42781 tgacttccag cgtagtgaag gcaaacttct cgccatcaaa tagcccctga ctggttagtt
    42841 ttagcgcggg gatcactggc agagaaagaa acgccatctg aataaacggc tcatcgggta
    42901 acggaccgca ttcacgggcg gcggctttca aggcgtcaat ttgttccgcc agcgactgcg
    42961 ccgtgtcggt gctcatcagg ccggcaatgg gcaacggcag atgactttgg acctggccgt
    43021 tacgtaccac gcacagcccg ccgccatcct gaatcacctg attgaccgcc agcgccatct
    43081 cttcggcact gcgaccgatc accacaatat tatggctgtc atggctgacc gtcgcagcca
    43141 gcgcaccttc attcagtcca aagccgccaa gcaaaccaca agccggagcc agccgttgcc
    43201 cgtaacgctc aagtacggca ataaagctca catcatcgcg atcaaaacca ttttcgctgt
    43261 agacgctgga gtgggagtgc gtaatcaatt cgttatggat gacgtcaatg acccgatagc
    43321 gttttccggg cgtaaattgc agggcaaagt cgctggcgga aactggctgg cgggcaatgg
    43381 tgttgccata tggcggagcg gattgtgcca gtctcgccga ctcttccgcc tgtaaggttt
    43441 gcgcatcaat cggctcgcct ttcaccagta cctgctgcac cgtgacctta cgcgcatcgc
    43501 tcaacaggac gatatcggcc tgcttgccgg gtgccagtaa gccgaggtga ttcagaccaa
    43561 agtggcgcgc cgtcgaccag ctggcgacgc gatatgccac atgcagcggc acattgtgtt
    43621 gttcgatcag gcggcgaatt aaggcatcga tgtgtccttc atgggcgatc tcccacgggt
    43681 tacggtcatc ggtacagagc atgcattgcg ggctgttaaa ttcgttgatc aacggtgcca
    43741 gcgcgttgag attgcgggca gcggaccctt cgcggatcat caacgacatg ccgagttgta
    43801 atttccggcg tccttcttcc agctgataac tttcgtggca gttttcaata cccgcagtaa
    43861 tataggcgtt aagttcttta ccacccaaac ccgggcagtg accgtccagc gtcaggtggc
    43921 gaaatgcatc cagtttatcg agcagcgcat tctgcccgct aattacgcca gggtagtcca
    43981 tcatttctgc aaggccggta acctgcggat ggtcccgcca ggcgagcatc tgttcaaggg
    44041 taaaactggc accgttaaca tcgcagcctt ccagggcggg tacgcaagag ctgacctgta
    44101 agtactggtt ttgccttgcc tgttcggcac agcgggcaaa ccaggcgaat ccggcttctc
    44161 ccatcacgtt gacgatttca tgagggtcgc aaataacggt cgtcaggccg cgcggcaggg
    44221 tagcggtttc aaaagtgacc ggcgtcatca tgctggattc aatatgcagg tgagcatcaa
    44281 taaaccctgg caccgccgtt gcgccgcgag catcaatccg ctgcaaagcc ggagcatcag
    44341 cgtattctgc gccaacaccg gcaatgtaac gtcctttaat cacaattggg ccggaaattt
    44401 ctccgccatt gatcaggtcg agaatagaga cattatcaat aatataatcg gcaacagcgt
    44461 cgccacggga aacggctaac aattcctggt attcagcccg gctaat
//
LOCUS       DAZGLJ010000016        41328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000016 DAZGLJ010000000
VERSION     DAZGLJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.016"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(219..431)
                     /gene="rpmE"
                     /locus_tag="ACHK49_000792"
     CDS             complement(219..431)
                     /gene="rpmE"
                     /locus_tag="ACHK49_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO9261481.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            634..2832
                     /gene="priA"
                     /locus_tag="ACHK49_000793"
     CDS             634..2832
                     /gene="priA"
                     /locus_tag="ACHK49_000793"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO9261482.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2988..4013
                     /gene="cytR"
                     /locus_tag="ACHK49_000794"
     CDS             2988..4013
                     /gene="cytR"
                     /locus_tag="ACHK49_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO9261483.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4105..5064
                     /gene="ftsN"
                     /locus_tag="ACHK49_000795"
     CDS             4105..5064
                     /gene="ftsN"
                     /locus_tag="ACHK49_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO9261484.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5157..5687
                     /gene="hslV"
                     /locus_tag="ACHK49_000796"
     CDS             5157..5687
                     /gene="hslV"
                     /locus_tag="ACHK49_000796"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO9261485.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5697..7028
                     /gene="hslU"
                     /locus_tag="ACHK49_000797"
     CDS             5697..7028
                     /gene="hslU"
                     /locus_tag="ACHK49_000797"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO9261486.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7095..8021
                     /gene="menA"
                     /locus_tag="ACHK49_000798"
     CDS             7095..8021
                     /gene="menA"
                     /locus_tag="ACHK49_000798"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO9261487.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8114..8599
                     /gene="rraA"
                     /locus_tag="ACHK49_000799"
     CDS             8114..8599
                     /gene="rraA"
                     /locus_tag="ACHK49_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO9261488.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8684..8929)
                     /gene="zapB"
                     /locus_tag="ACHK49_000800"
     CDS             complement(8684..8929)
                     /gene="zapB"
                     /locus_tag="ACHK49_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO9261489.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9354..10199
                     /gene="glpF"
                     /locus_tag="ACHK49_000801"
     CDS             9354..10199
                     /gene="glpF"
                     /locus_tag="ACHK49_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO9261490.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10222..11730
                     /gene="glpK"
                     /locus_tag="ACHK49_000802"
     CDS             10222..11730
                     /gene="glpK"
                     /locus_tag="ACHK49_000802"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO9261491.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11865..12875
                     /gene="glpX"
                     /locus_tag="ACHK49_000803"
     CDS             11865..12875
                     /gene="glpX"
                     /locus_tag="ACHK49_000803"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO9261492.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12972..13718
                     /gene="fpr"
                     /locus_tag="ACHK49_000804"
     CDS             12972..13718
                     /gene="fpr"
                     /locus_tag="ACHK49_000804"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO9261493.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13723..14151)
                     /gene="uspD"
                     /locus_tag="ACHK49_000805"
     CDS             complement(13723..14151)
                     /gene="uspD"
                     /locus_tag="ACHK49_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO9261494.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14178..14477)
                     /gene="yiiS"
                     /locus_tag="ACHK49_000806"
     CDS             complement(14178..14477)
                     /gene="yiiS"
                     /locus_tag="ACHK49_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO9261495.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14689..15129)
                     /gene="yiiR"
                     /locus_tag="ACHK49_000807"
     CDS             complement(14689..15129)
                     /gene="yiiR"
                     /locus_tag="ACHK49_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO9261496.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15230..15829
                     /gene="yiiQ"
                     /locus_tag="ACHK49_000808"
     CDS             15230..15829
                     /gene="yiiQ"
                     /locus_tag="ACHK49_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO9261497.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            15937..16704
                     /gene="tpiA"
                     /locus_tag="ACHK49_000809"
     CDS             15937..16704
                     /gene="tpiA"
                     /locus_tag="ACHK49_000809"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO9261498.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16759..17514)
                     /gene="cdh"
                     /locus_tag="ACHK49_000810"
     CDS             complement(16759..17514)
                     /gene="cdh"
                     /locus_tag="ACHK49_000810"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO9261499.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(17621..18610)
                     /gene="sbp"
                     /locus_tag="ACHK49_000811"
     CDS             complement(17621..18610)
                     /gene="sbp"
                     /locus_tag="ACHK49_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO9261500.1"
                     /translation="MNKWGVGLTFLLAATNVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLV
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18929..19891)
                     /gene="pfkA"
                     /locus_tag="ACHK49_000812"
     CDS             complement(18929..19891)
                     /gene="pfkA"
                     /locus_tag="ACHK49_000812"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO9261501.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20072..20974)
                     /gene="fieF"
                     /locus_tag="ACHK49_000813"
     CDS             complement(20072..20974)
                     /gene="fieF"
                     /locus_tag="ACHK49_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO9261502.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21123..21623)
                     /gene="cpxP"
                     /locus_tag="ACHK49_000814"
     CDS             complement(21123..21623)
                     /gene="cpxP"
                     /locus_tag="ACHK49_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO9261503.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21773..22471
                     /gene="cpxR"
                     /locus_tag="ACHK49_000815"
     CDS             21773..22471
                     /gene="cpxR"
                     /locus_tag="ACHK49_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO9261504.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22468..23841
                     /gene="cpxA"
                     /locus_tag="ACHK49_000816"
     CDS             22468..23841
                     /gene="cpxA"
                     /locus_tag="ACHK49_000816"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO9261505.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(23947..24621)
                     /gene="yiiM"
                     /locus_tag="ACHK49_000817"
     CDS             complement(23947..24621)
                     /gene="yiiM"
                     /locus_tag="ACHK49_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO9261506.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24770..25753)
                     /gene="kdgT"
                     /locus_tag="ACHK49_000818"
     CDS             complement(24770..25753)
                     /gene="kdgT"
                     /locus_tag="ACHK49_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO9261507.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26013..26633)
                     /gene="sodA"
                     /locus_tag="ACHK49_000819"
     CDS             complement(26013..26633)
                     /gene="sodA"
                     /locus_tag="ACHK49_000819"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO9261508.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            26918..27952
                     /gene="rhaT"
                     /locus_tag="ACHK49_000820"
     CDS             26918..27952
                     /gene="rhaT"
                     /locus_tag="ACHK49_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO9261509.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWSNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(27949..28797)
                     /gene="rhaR"
                     /locus_tag="ACHK49_000821"
     CDS             complement(27949..28797)
                     /gene="rhaR"
                     /locus_tag="ACHK49_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO9261510.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQHVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLAMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(28871..29708)
                     /gene="rhaS"
                     /locus_tag="ACHK49_000822"
                     /pseudo
     CDS             complement(28871..29708)
                     /gene="rhaS"
                     /locus_tag="ACHK49_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
     gene            29996..31465
                     /gene="rhaB"
                     /locus_tag="ACHK49_000823"
     CDS             29996..31465
                     /gene="rhaB"
                     /locus_tag="ACHK49_000823"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO9261511.1"
                     /translation="MTFRNCVAVNLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDNF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            31462..32721
                     /gene="rhaA"
                     /locus_tag="ACHK49_000824"
     CDS             31462..32721
                     /gene="rhaA"
                     /locus_tag="ACHK49_000824"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO9261512.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARQALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            32804..33628
                     /gene="rhaD"
                     /locus_tag="ACHK49_000825"
     CDS             32804..33628
                     /gene="rhaD"
                     /locus_tag="ACHK49_000825"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO9261513.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAEVLVKIYSMGGMKQTITREELIALGERFGVTPLASALAL"
     gene            33700..34848
                     /gene="fucO"
                     /locus_tag="ACHK49_000826"
     CDS             33700..34848
                     /gene="fucO"
                     /locus_tag="ACHK49_000826"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO9261514.1"
                     /translation="MSFMLALPKISLHGAGAIGDMVNLVAHKQWGKALIVTDGQLVKL
                     GLLDSLFIALDQYEMSYHLFDEVFPNPTEELVQKGYAAFQDAACDYIIAFGGGSPIDT
                     AKAVKILTANPAPSTAYSGVGKVTNPGVPLVAINTTAGTAAEMTSNAVIIDTARQVKE
                     VIIDPNIIPDIAVDDASVMLEIPASVTAATGMDALTHAVEAYVSVGAHPLTDANALEA
                     IRLITLWLPKAVDDGHNLEAREQMAFAQYLAGMAFNSAGLGLVHALAHQPGATHNLPH
                     GVCNAILLPVIENFNRPNAIARFARIAQAMGVDTRDMSEEAASTEAINAIRALSHRVG
                     IPAGFSQLGITKEDIEGWLDKALADPCVPCNPRTASRDEVRELYLEAL"
     gene            34845..35159
                     /gene="rhaM"
                     /locus_tag="ACHK49_000827"
     CDS             34845..35159
                     /gene="rhaM"
                     /locus_tag="ACHK49_000827"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO9261515.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            35472..35918
                     /gene="frvA"
                     /locus_tag="ACHK49_000828"
     CDS             35472..35918
                     /gene="frvA"
                     /locus_tag="ACHK49_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO9261516.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFINQLRQGDADQVLALLNQTLSS"
     gene            35929..37380
                     /gene="frvB"
                     /locus_tag="ACHK49_000829"
     CDS             35929..37380
                     /gene="frvB"
                     /locus_tag="ACHK49_000829"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO9261517.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            37370..38440
                     /gene="frvX"
                     /locus_tag="ACHK49_000830"
     CDS             37370..38440
                     /gene="frvX"
                     /locus_tag="ACHK49_000830"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO9261518.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPQSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAKH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYFPNQKLVAALKSCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVSAFSQFRQVD"
     gene            38440..40188
                     /gene="frvR"
                     /locus_tag="ACHK49_000831"
     CDS             38440..40188
                     /gene="frvR"
                     /locus_tag="ACHK49_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO9261519.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEII
                     AGLTGYEAFMELLHKG"
     gene            complement(40238..41236)
                     /gene="yiiG"
                     /locus_tag="ACHK49_000832"
     CDS             complement(40238..41236)
                     /gene="yiiG"
                     /locus_tag="ACHK49_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO9261520.1"
                     /translation="MALGLTGCDDKKAETETPPPANSQPAAPAPEAPVAKAEAKPETP
                     AQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQGPTGEERTVYGIY
                     GISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMDKYYTQENYK
                     DDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELKNIEEREGKTFHYY
                     SLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMNFSFINSAGQY
                     QLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT        10061 a        10667 c        10673 g         9927 t
ORIGIN      
        1 acaatgctac tgccatactg cgtcatgtcg ccctgacgcg ctgccggttt tccgctcatc
       61 gtaaaatcct tttgaaataa ataagaacca gaagcctagc gccagtcaac aacaggaaat
      121 gataatgagt taacaccccg gaatttttca gaatttataa aaggcaaatt acaggcacaa
      181 aaaaagcgcc gtgcggcgct tttttcggaa atccggtctt atttgctgcc cgggatgttg
      241 aaacgcttgt tgaagcggtc aacacggcca ccggtagcaa catcacgctg tttgccagtg
      301 aagaacgggt ggcacttgct gcacacgtcg aggttcaggt catgaccaac ggtggagcgg
      361 attttcatta cgttaccgca agagcagcta gcagtaattt cttcgtattt cgggtgaata
      421 tcttttttca tgggaaaacc tcggtttaag gccgcgtcgc tcttccagcc ctaacgccag
      481 acaccacgcg atgttaaaag tatagcttca atacgatcat ttcgtacgaa ggcgcgaaat
      541 catacagaaa ttaaccagcg tatgcaaact gatccgcact cttctacggc aatgtgtata
      601 ctaacccacc gaatttcaag tcaggatgat gctatgcccg tagcccacgt tgccttgccc
      661 gttccgcttc ctcgtacctt tgactatctg ctgccagaag gcatgacggt taaagctggg
      721 tgtcgcgtgc gcgtgccgtt tggcaaacag caggagcgca tcgggattgt ggtatcagtt
      781 agcgatgcca gcgaactgcc gctcaatgag ctaaaagcgg tagtcgaagt gctggacgtt
      841 gagccggtat ttactcactc cgtctggcgg ttactgctct gggcggcaga ttactatcat
      901 catccgattg gcgatgtgct gtttcatgcc ttgccgattt tactgcgcca ggggcggcct
      961 gcggcgaacg cgccgatgtg gtactggttt gccactgaac aaggtcaggc ggtggatctt
     1021 aacagcctga aacgttcgcc aaagcaacaa caggcgctgg cggcgttacg gcaaggcaaa
     1081 atctggcgcg accaggtcgc ggaacttgaa tttaatgatg ccgcgttgca ggcgctgcgc
     1141 aaaaaaggtc tgtgtgattt agcaagtgaa acaccagagt ttagcgactg gcgaacgaac
     1201 tatgccgttt ctggtgagcg gttgcgattg aataccgaac aagccaccgc cgttggcgca
     1261 attcatagcg cggcagatac tttttctgcc tggctgctgg cgggcgttac cggttccggt
     1321 aaaacggagg tttatctcag tgtactggaa aacgtgctcg ctcagggcaa acaggcgctg
     1381 gtgatggtgc cggaaatcgg cctgacaccg caaactatcg cccgttttcg tgaacgtttt
     1441 aatgcccccg tggaagttct gcattccggc ctgaacgaca gcgagcggct ttcggcgtgg
     1501 ttgaaagcga aaaatggcga ggcggcgatt gtgatcggca cccgctccgc gctgtttacg
     1561 ccgtttaaaa atctcggcgt gattgtcatt gatgaagagc acgacagctc ctacaagcag
     1621 caggaaggct ggcgctatca tgcccgcgac ctggcggtgt atcgtgcgca tagcgagcaa
     1681 atcccgatta ttcttggctc cgcaacgccc gcgctggaaa cgttatgcaa cgtccagcag
     1741 aaaaaatacc gcctgctgcg cctgacccgt cgggcaggga atgcgcgtcc ggcaattcaa
     1801 catgtgctgg atttaaaagg tcagaaggtg caggcaggtc tggctccggc gttaatcacc
     1861 cgtatgcgcc agcatttaca ggccaacaat caggttatcc tctttcttaa tcgtcggggt
     1921 tttgcgcctg cgctgctgtg ccacgactgc ggctggatag ccgaatgccc acgttgcgat
     1981 cactactaca cgctgcatca ggcgcaacag catttgcgct gtcaccactg tgacagtcag
     2041 cgtccggtgc cgcgccagtg cccttcctgc ggttccacgc acctggtccc cgtggggctg
     2101 ggcaccgaac agcttgaaca gacgcttgcg ccgatgttcc ccggcgtgcc catttcacgt
     2161 atcgaccgcg ataccaccag ccgcaaaggg gcgctggaac agcaactggc ggaagtgcat
     2221 cgcggcggcg cgaggatctt gattggcacg caaatgctgg cgaaaggtca ccactttccg
     2281 gatgtgacgc tggtggcgtt actggatgtg gacggcgcgc tgttttctgc cgattttcgc
     2341 tcggcagaac gtttcgccca gctttacacc caggtcgccg gtcgtgccgg gcgtgcgggt
     2401 aaacagggcg aagtggtgct gcaaacgcac catccggaac atcctctgtt gcaaacgttg
     2461 ctctataaag gctacgacgc ctttgccgaa caggcgctgg ctgagcgacg catgatgcag
     2521 ctgccgccgt ggaccagcca tgtgattgtg cgtgcggaag atcataacaa tcagcatgcg
     2581 ccgctgttcc tgcaacaact gcgtaatctg atcctttcca gcccgctggc agacgacaaa
     2641 ctgtgggttc tcggcccggt tccggctctg gcacctaagc gtggtggccg ctggcgctgg
     2701 cagatcttgt tgcagcaccc ttcccgcgtg cgcttgcaac acatcattag tggtacgctg
     2761 gcgcttatta atacaatacc ggattcccgc aaggtgaaat gggtgttgga tgtcgatccc
     2821 atcgaaggct aatgcgcaaa cgttgcgagg cggatcgaaa aattcaatat tcatcacact
     2881 tttcatgaaa attctgtaac cgttttcacg cgctatctgc taaaaatgtt gccgatgtga
     2941 agtaaacatg gatgtagtac gcctgacgtg ccaggcgagg agtgagtgtg aaagcgaaga
     3001 agcaggaaac tgccgcgacc atgaaagacg ttgccctcaa ggcaaaagtc tctacagcga
     3061 ctgtctcccg agcattaatg aatcccgata aagtctccca ggccacccgt aatcgggttg
     3121 aaaaagcggc ccgggaagtg ggttatttac cgcagcctat ggggcgcaac gtcaagcgta
     3181 atgaatcccg caccattctg gtgattgtcc cggatatctg cgatcccttc tttagcgaaa
     3241 ttattcgcgg tatcgaagtt acggcggcaa atcacggata tctggtgctg attggcgact
     3301 gtgcgcatca aaatcagcag gaaaaaacct ttatcgattt gatcatcacc aagcaaattg
     3361 atggcatgtt gctgctgggt tcaaggctgc cgtttgatgc cagcattgag gaacagcgta
     3421 atctgccgcc gatggtgatg gcgaacgaat tcgcaccgga actggagcta cctacagttc
     3481 atatcgacaa tctgaccgcc gcatttgatg cagtaaatta tctatatgag caagggcata
     3541 aacggattgg ctgtatagcc ggtcccgaag agatgccgct gtgtcactac cgcctgcaag
     3601 gttacgttca ggcgctgcgt cgctgcggca ttatggttga tccgcaatac atcgcccgtg
     3661 gcgacttcac cttcgaagcc ggaagcaaag cgatgcagca gctgcttgat cttccacaac
     3721 cgcctactgc tgtcttctgc catagcgatg tgatggcgct cggcgcactt tctcaggcaa
     3781 aacgccaggg gctgaaagtc ccggaagacc tttccataat cggttttgat aacatcgacc
     3841 tgacgcaatt ttgtgatccg ccgctgacaa ccatcgcgca gccgcgttac gaaatcggtc
     3901 gggaagctat gctgttattg cttgatcaaa tgcaggggca acacgttggc agtggctctc
     3961 gtttaatgga ctgcgaactt atcatccggg gatcaacacg cgcgttacct taaagtaaac
     4021 ctttaagact gccgtgactg gtcaaaggcc cgccgcttaa gtaacatggc gggctgacga
     4081 acgaataaat acagcgaaac gatagtggca caacgagatt atgtacgccg cagccaaccg
     4141 gcaccttcgc ggcgaaaaaa gagcacctca cggaaaaagc aacgaaatct gcctgcggtt
     4201 tctcccgcta tggtcgctat tgctgccgcc gttcttgtga cctttatcgg tggtctgtac
     4261 ttcattacgc atcacaagaa agaagagtcc gagacgctgc aaagccagaa agtgaccggt
     4321 aacggactac caccaaaacc agaagaacgc tggcgctaca ttaaagagct ggaaagtcgc
     4381 cagccgggtg tgcgtgcgcc cacagaacct tctgccggtg gtgaagtgaa aacgccggag
     4441 caactgacac cagaacaacg tcagcttctt gaacaaatgc aggctgatat gcgccagcag
     4501 ccaacgcagc tggttgaagt tccgtggaat gaacagacgc cggaacagcg tcagcaaacg
     4561 ctacaacgcc agcgtcaggc gcagcaatta gcggagcagc aacgcctggc tcagcagtcg
     4621 cgtacgactg aacaaagctg gcagcagcag acgcgtacgt cgcaagccgc gccggtacag
     4681 gcgcaaccgc gccagtctaa accggcttct actcagcagc cgtaccagga tctgctgcaa
     4741 acccctgcgc acacgaccgc gcagtcgaag ccgcagcaag ctgcgccggt tgctcgtgcc
     4801 gctgacgcgc caaaaccgac ggcggagaaa aaagacgaac gccgctggat ggtgcagtgc
     4861 ggttcgttca gaggcgcgga acaggcagag acggtacgtg ctcagctggc gttcgaagga
     4921 tttgactcga aaatcaccac caacaatggc tggaatcgtg tggtcattgg cccggtgaaa
     4981 ggcaaagaga acgcagacag caccctcaat cggttgaaga tggcgggtca tacaaactgc
     5041 attcggctcg ccgccggggg ttgaaaccct caaaatcccc cccatctata attgcattat
     5101 gccccgtact tttgtacggg gtttgtactc tgtattcgta accaaggggt cagctcgtga
     5161 caactatagt aagcgtacgc cgtaacggcc atgtggtcat cgctggtgat ggtcaggcca
     5221 cgttgggcaa taccgtaatg aaaggcaacg tgaaaaaggt ccgccgtctg tacaacgaca
     5281 aagtcatcgc gggctttgcg ggcggtactg cggatgcttt tacgctgttc gaactgtttg
     5341 aacgtaaact ggaaatgcat cagggccatc tggtcaaagc cgccgttgag ctagcgaaag
     5401 actggcgtac cgatcgcatg ctgcgcaaac ttgaagcact gctggcagtc gcggatgaaa
     5461 ctgcatcgct tatcatcacc ggtaacggtg acgtggtgca gccagaaaac gatcttattg
     5521 ctatcggctc cggcggccct tacgcccagg ctgcggcgcg tgcactgtta gaaaatactg
     5581 aactgagtgc tcgtgaaatt gctgaaaagg cgttggatat tgcaggcgac atttgcatct
     5641 ataccaacca tttccacacc atcgaagaat taagctacaa agcgtaagga tctcccatgt
     5701 ctgaaatgac cccacgcgaa atcgtcagcg aactggataa gcacatcatc ggccaggaca
     5761 acgccaagcg ttctgtggcg attgctctgc gtaaccgctg gcgtcgcatg cagctcaacg
     5821 aagagctgcg ccatgaagtg accccgaaaa atatcctgat gatcggcccg accggtgtcg
     5881 gtaaaactga aatcgcccgt cgtctggcta agctggcgaa tgcgccgttc atcaaagttg
     5941 aagcgaccaa attcaccgaa gtaggctacg tcggtaagga agtggattct attattcgcg
     6001 atctgaccga tgccgccgtg aaaatggtac gcgtccaggc tatcgagaaa aaccgttatc
     6061 gcgctgaaga actggcagaa gaacgtattc tcgacgtgct gatcccacct gctaaaaaca
     6121 actggggaca gaccgaacag cagcaggaac cgtccgctgc tcgtcaggca ttccgcaaaa
     6181 aactgcgtga aggccagctt gatgacaaag aaatcgagat cgatcttgcc gcagcaccga
     6241 tgggcgttga aattatggct cctccaggca tggaagagat gaccagccag ctgcagtcca
     6301 tgttccagaa cctgggcggc cagaagcaaa aagcgcgtaa gctgaaaatc aaagacgcca
     6361 tgaagctgct gattgaagaa gaagcggcga aactggtgaa cccggaagag ctgaagcaag
     6421 acgctatcga cgctgttgag cagcacggga tcgtgtttat cgacgaaatc gacaaaatct
     6481 gtaagcgcgg cgagtcttcc ggtccggatg tttctcgtga aggcgttcag cgtgacctgc
     6541 tgccgctggt agaaggttgc accgtttcca ccaaacacgg gatggtcaaa actgaccaca
     6601 ttctgtttat cgcttctggc gcgttccaga ttgcgaaacc gtctgaccta atcccggaac
     6661 tgcaaggtcg tctgccaatc cgcgttgaac tgcaggcact gaccaccagc gacttcgagc
     6721 gtattctgac cgagccgaat gcctctatca ccgtgcagta caaagcactg atggcgactg
     6781 aaggcgtaaa tatcgagttt accgactccg gtattaagcg catcgcggaa gcggcatggc
     6841 aggtgaacga atctaccgaa aacatcggtg ctcgtcgttt acacactgtt ctggagcgtt
     6901 taatggaaga gatttcctac gacgccagcg atttaagcgg tcaaaatatc actattgacg
     6961 cagattatgt gagcaaacat ctggatgcgt tggtggcaga tgaagatctg agccgtttta
     7021 tcctataatc gcgttcaatc attttcatca ttgtttgatg gggctgaaag gccccatttt
     7081 tattggcgcg tattatgact gaacaacaaa ttagccgaac tcaggcgtgg ctggaaagtt
     7141 tacgacctaa aaccctcccc ctcgcctttg ctgcaattat cgtcgggacg gcgctggcat
     7201 ggtggcaagg tcacttcgat ccgctggtcg ccctgctggc actaattacc gccgggctat
     7261 tacagatcct ttctaacctc gccaatgatt acggcgatgc ggtaaaaggc agcgataaac
     7321 ctgaccgcat tgggccgcta cgcggcatgc aaaaaggggt cattacccag caagagatga
     7381 aacgggcgct cattattacc gtcgtgctca tctgtctctc cgggttggca ctggttgcag
     7441 tggcatgcca tacgctggcc gattttgtcg gtttcctgat tcttggcggg ttgtcgatca
     7501 ttgccgctat cacctacacc gtgggcaatc gtccttatgg ttatatcggt ctgggtgata
     7561 tttccgtact ggttttcttt ggctggttga gtgtcatggg gagctggtat ttacaggctc
     7621 atacattgat tccggcactg atccttccgg cgaccgcatg cggcctgctg gcaacggcag
     7681 tactgaatat taataacctg cgtgatatca atagcgaccg cgaaaatggc aaaaacacgc
     7741 tggtggtgcg cttaggtgaa gtgaacgcgc gtcgttatca tgcctgcctg ctgatgggct
     7801 cgctggtgtg tctggcgctg tttaatctct tttcgctgca tagcctgtgg ggctggctgt
     7861 tcctgctggc ggcaccatta ctggtgaagc aagcccgtta tgtgatgcgg gaaatggacc
     7921 cggtggcgat gcgaccaatg ctggaacgta ctgtcaaggg agcgttactg actaacctgc
     7981 tgtttgtttt agggatattc ctaagccagt gggcagcata actgacaaat atcaattaac
     8041 aattgatgat tttgccaaca gcccacatag cgcgatatac tgaaaattct cgcagcaact
     8101 gaatgttaag cctatgaaat acgatacttc cgagctttgt gacatctatc aagaagatgt
     8161 taacgtcgtg gaaccgctgt tctccaactt tggcggacgg gcgtcgtttg gcggacaaat
     8221 aatcacggta aaatgtttcg aggacaacgg gttgctgtac gatctgctcg aacagaatgg
     8281 ccgtggtcgt gttcttgtcg tcgatggcgg tggttctgtt cgtcgcgcac tggtcgatgc
     8341 tgaactggcg cgtctggcag tacaaaatga atgggaaggt ctggtcattt acggcgcggt
     8401 gcgtcaggta gatgacctgg aagagttgga tatcggcatc caggcgatgg cggcaattcc
     8461 ggttggtgcc gctggcgaag gcattggcga aagcgatgtc cgcgtcaatt ttggcggtgt
     8521 caccttcttc tccggcgacc atctttatgc cgacaatacc gggattattc tttctgaaga
     8581 tccgctggat attgaatgat agaaaaggca ccgcaaggtg ccttttttct gcgttacctg
     8641 ttggcctaca cagtaaagaa attacgcgga agatgaagcg taatcagacc tcttccatgc
     8701 gacccagcag ggcctgcaga cgttcctgcc agccgttctg ctgttctttc agatggttgt
     8761 tctcacgctc cagctcttcg cgctgatgct gggcattttg aacttcctgc gacagtgagt
     8821 tgtttttttc tttcagctct tcgatttcca tctgcaacag agtgatggta tcaatcgcct
     8881 gctgtacttt tgcttccagt ttctcaaaca cttctaatga cattgtcata cctctcctga
     8941 attgcaaggc gttgatggat aaaaatcctc gtcccgatta ccggtgacgc cttaataaat
     9001 acgagcgcac tttagttagc tccgattgta tgaagccgcg ccatcgctgt ccagcggcac
     9061 gccttgcaga ttacggtttg ccacactttt catccttctc ctggtgacat aatccacatc
     9121 aatcgaaaat gttaataaat ttgttgcgcg aatgatctaa caaacatgca tcatgtacaa
     9181 tcagatggaa taaatggcgc gataacgctc attttatgac gaagcacaca cattttaagt
     9241 tcgatatttc tcgcttttgc tcgttaacga taagtttaca gcatgcctac aagcatcgtg
     9301 gaggtccgtg actttcacgc atacaacaaa cattaactct tcaggatccg attatgagtc
     9361 aaacatcaac cttgaaaggc cagtgcattg ctgaattcct cggtaccggg ttgttgattt
     9421 tcttcggtgt gggttgcgtt gcagcactaa aagtcgctgg tgcgtctttt ggtcagtggg
     9481 aaatcagtgt catttgggga ctgggggtgg caatggccat ctacctgacc gcaggggttt
     9541 ccggcgcgca tcttaatccc gctgttacca ttgcattgtg gctgtttgcc tgtttcgaca
     9601 agcgcaaagt tattcctttt atcgtttcac aagttgccgg cgctttctgt gctgcggctt
     9661 tagtttacgg gctttactac aatttatttt tcgacttcga gcagactcat cacattgttc
     9721 gcggcagcgt tgaaagtgtt gatctggctg gcactttctc tacttaccct aatcctcata
     9781 tcaattttgt gcaggctttc gcagttgaga tggtgattac cgctattctg atggggctga
     9841 tcctggcgtt aacggatgat ggcaacggtg taccacgcgg ccctttggct cccttgctga
     9901 ttggtctact gattgcggtc attggcgcat ctatgggccc attgacaggt tttgccatga
     9961 acccagcgcg tgacttcggt ccgaaagtct ttgcctggct ggcgggctgg ggcaatgtcg
    10021 cctttaccgg cggcagagac attccttact tcctggtgcc gcttttcggc cctatcgttg
    10081 gcgcgattgt aggtgcattt gcctaccgca aactgattgg tcgccatttg ccttgcgata
    10141 tctgtgttgt ggaagaaaag gaaaccacaa ctccttcaga acaaaaagct tcgctgtaat
    10201 atgactacgg gacaattaaa catgactgaa aaaaaatata tcgttgcgct cgaccagggc
    10261 accaccagct cccgcgcggt cgtaatggat cacgatgcca atatcattag cgtgtcgcag
    10321 cgcgaatttg agcaaatcta cccaaaacca ggttgggtag aacacgaccc aatggaaatc
    10381 tgggccaccc aaagctccac gctggtagaa gtgctggcga aagccgatat cagttccgat
    10441 caaattgcag ctatcggtat tacgaaccag cgtgaaacca ctattgtctg ggaaaaagaa
    10501 accggcaagc ctatctataa cgccattgtc tggcagtgcc gtcgtaccgc agaaatctgc
    10561 gagcatttaa aacgtgacgg tttagaagat tatatccgca gcaataccgg tctggtgatt
    10621 gacccgtact tttctggcac caaagtgaag tggatcctcg accatgtgga aggctctcgc
    10681 gagcgtgcac gtcgtggtga attgctgttt ggtacggttg atacgtggct tatctggaaa
    10741 atgactcagg gccgtgtcca tgtgaccgat tacaccaacg cctctcgtac catgttgttc
    10801 aacatccata ccctggactg ggacgacaaa atgctggaag tgctggatat tccgcgcgag
    10861 atgctgccag aagtgcgtcg ttcttccgaa gtatacggtc agactaacat tggcggcaaa
    10921 ggcggcacgc gtattccaat ctccgggatc gccggtgacc agcaggccgc gctgtttggt
    10981 cagttgtgcg tgaaagaagg gatggcgaag aacacctatg gcactggctg ctttatgctg
    11041 atgaacactg gcgagaaagc ggtgaaatca gaaaacggcc tgctgaccac catcgcctgc
    11101 ggcccgactg gcgaagtgaa ctatgcgttg gaaggtgcgg tgtttatggc aggcgcatcc
    11161 attcagtggc tgcgcgatga gatgaagttg attaacgacg cctacgattc cgaatatttc
    11221 gccaccaaag tgcaaaacac caatggtgtg tatgtggttc cggcatttac cgggctgggt
    11281 gcgccgtact gggacccgta tgcgcgcggg gcgattttcg gtctgactcg tggggtgaac
    11341 gctaaccaca ttatacgcgc gacgctggag tctattgctt atcagacgcg tgacgtgctg
    11401 gaagcgatgc aggccgactc tggtatccgt ctgcacgccc tgcgcgtgga tggtggcgca
    11461 gtagcaaaca atttcctgat gcagttccag tccgatattc tcggcacccg cgttgagcgc
    11521 ccggaagtgc gcgaagtcac cgcattgggt gcggcctatc tcgcaggcct ggcggttggc
    11581 ttctggcaga acctcgacga gctgcaagag aaagcggtga ttgagcgcga gttccgtcca
    11641 ggcatcgaaa ccactgagcg taattaccgt tacgcaggct ggaaaaaagc ggttaaacgc
    11701 gcgatggcgt gggaagaaca cgacgaataa tgtaaatgcc gaatgaagcg tttatgccgc
    11761 atccggtagt cccgaaacgt gcgggggcaa ccccgcacac atcaataatc cctcccttcc
    11821 cctgtgctac acttcgcgcc attccttact gcttagagtt tgctatgaga cgagaacttg
    11881 ccatcgaatt ttcccgcgtc accgaatcag cggcgctggc tggctacaaa tggttaggac
    11941 gcggcgataa aaacaccgcg gacggcgcgg cggtaaacgc catgcgtatt atgctcaacc
    12001 aggtcaacat tgacggcacc atcgtcattg gtgaaggtga aatcgacgaa gcaccgatgc
    12061 tctacattgg tgaaaaagtc ggtactggtc gcggcgacgc ggtagatatt gctgttgatc
    12121 cgattgaagg cacgcgcatg acggcgatgg gccaggctaa cgcgctggcg gtgctggcag
    12181 taggcgataa aggctgcttc ctcaatgcgc cggatatgta tatggagaag ctgattgtcg
    12241 ggccgggagc caaaggcacc attgatctga acctgccgct ggcggataac ctgcgcaatg
    12301 tagcggcggc gctcggcaaa ccgttgagcg aactgacggt aacgattctg gctaaaccac
    12361 gccacgatgc cgttatcgct gaaatgcagc aactcggcgt acgcgtattt gctattccgg
    12421 atggcgacgt tgcggcctca attctcacct gtatgccaga cagcgaagtt gacgtgctgt
    12481 acggtattgg tggcgcgccg gaaggcgtag tttctgcggc ggtgatccgc gcattagatg
    12541 gcgacatgaa cggtcgtctg ctggcgcgtc atgacgtcaa aggcgacaac gaagagaatc
    12601 gtcgcattgg cgagcaggag ctggcacgct gcaaagcgat gggcatcgaa gccggtaaag
    12661 tattgcgcct gggcgatatg gcgcgcagcg ataacgtcat cttctctgcc accggtatta
    12721 ccaaaggcga tctgctggaa ggcattagcc gcaaaggcaa tatcgcgact accgaaacgc
    12781 tgctgatccg cggcaagtca cgcaccattc gccgcattca gtccatccac tatctggatc
    12841 gcaaagaccc ggaaatgcag gtgcacatcc tctgattgat ttgatcgatt gagccttcca
    12901 gtccttcggg actggaattt ttttgttcgg agaacgaaga taaggcaagt caatcaaaac
    12961 aggagaaaaa catggctgat tgggtaacag gcaaagtcac taaagtgcag aactggaccg
    13021 acgccctgtt tagtctcacc gttcacgccc ccgtgcttcc gtttaccgcc gggcaattca
    13081 ccaagcttgg ccttgaaatc gacggcgaac gcgtccagcg cgcctactcc tatgttaact
    13141 cgcccgataa tcccgatctg gagttttacc tggtcaccgt ccccgatggc aaattaagcc
    13201 cacgattggc ggcactgaaa ccaggcgatg aagtgcaggt ggttagcgaa gcagctggct
    13261 tctttgttct ggatgaagta ccggactgcg aaacgctatg gatgctggca accggtacag
    13321 cgattggccc ttatttatcg attctgcaac taggcaaaga tttagatcgc ttcaaaaatc
    13381 tggtcctggt tcacgccgca cgttatgccg ccgacttaag ctatttgcca ctgatgcaag
    13441 aactggaaaa acgctacgaa gggaaactgc gcattcagac ggtggtcagt cgggaaacgg
    13501 cagcggggtc gctcaccggg cggataccgg cgttaattga aagtggggaa ctggaaagcg
    13561 cgattggcct gccgatgaat aaagaaacca gccatgtgat gctgtgcggc aatccacaga
    13621 tggtgcgcga tacacaacag ttgctgaaag agacccggca gatgacgaaa catttacgtc
    13681 gccgaccggg ccatatgaca gcggagcatt actggtaagc ggttacttat cgataaacgg
    13741 cacgatgagc aaatccgcac tcatcttatt gatcatcccg cgatatgccg gcatcaaacg
    13801 gttgataaat gagtgatgat gaccacagac aaggaggtcg cactgctctt tttgcataat
    13861 ttccagcagt gtttccggca tttctccgcg ttcaatacgc agttttgtct tcggccattg
    13921 aatatttttc gtcagtttat acagcttgtt atccgactta ttcttcaaca attgaagaat
    13981 atcttctgtt gcagggaagt agatacccgg atacaactcg cttaagccat catcaatatg
    14041 aattaacgtc aggtgagcgt cattatgtct ggcgagctcc agagctttat tcaccagtaa
    14101 ggcatcttct tcattcccgg aaattgccac gccaatgtgt ttataagcca tgtttaactc
    14161 cttctaaagc aactccatca agctaacgaa cgcagtgata actcaaaaat aatcatctcg
    14221 gcctggcatg agaaagtgaa ggccgcatca agctcaactt gcccctccag atccttaatc
    14281 ttgtaggcca cgctggcccc ttcatctgca gaggatgcag cggcggcaag ctctttcagt
    14341 ttggtcataa aagactctgc ttcttcgcgg gtggcaaaaa agcgcgaaaa cttactggta
    14401 cagttgtcgt tatcaatcac cgtaccgata tcaatcgcac atcctttagt actgcattta
    14461 tctacgacat ctttcatagg gcacctctgt atattcgcct ctctgtcgat tacgacttaa
    14521 gaataatgcg ccgttaaggg aagaacaaac aagtaatgat cttatggatt accatttttt
    14581 ttgatattgc ttaaggtgaa gagttcagaa ggattttccg ggtattgtca tttgcatcgt
    14641 ggaaataaca atcccggaaa aaaatagcag taagagatat attaaaggtt aattacttga
    14701 tttattgtcg gctttatact tcacatcctg agtatcttta ccatatttat tttcgccttg
    14761 tgtgccaaca aacgcaccaa gatcgataag catcatcacc agaatcaacg tcgggacaaa
    14821 acgccccacc gcccattgcc agacacccgg taaaatcgcc cagttacccg ccagcagcat
    14881 ccacgccaca atcatcagaa atgcccatgc gccggaacgc ccgcgatcat gcaagcgctt
    14941 aacagttact gccgctgttg gccaaagcaa gcacacaagg caaaacgccg cggtctgaat
    15001 atcgagtaaa tttttacccg ccagtgagaa aagcaccagc atgcctgcga accacaggcc
    15061 tatccaaatc cagaaatcac ggcgtccaat acgcccttta aatgagaata accattgctg
    15121 tatggtcatg taagttcctt gatggttgtc ttttccagga ttctaccctt ttgacaaggg
    15181 cgacagatat cgttttaatc ggagccagtc ataacaaaag gtactgtcaa tgaagccagg
    15241 gtgtacgctg ttttttctct tatgttctgc attaaccgtt acaacaacgg cgcatgcaca
    15301 aacaccagat acggcaacga ccgcgcctta tctgctggct ggagccccta ctttcgatct
    15361 ctccatcagc cagtttcgag aagactttaa cagccagaat cctagcctgc cactgaacga
    15421 atttcgtgcc atcgacagca gtcccgacaa agccaatctc actcgtgctg caagtaaaat
    15481 taacgagaac ttgtatgctt ctacagcgct ggagcgcggt accttaaaaa tcaaaagcat
    15541 tcaaatgacc tggctaccca tccaggggcc agagcaaaaa gcagcgaaag cgaaagctca
    15601 ggaatacatg gcagcagtga tccgcacact caccccatta atgaccaaaa cacaaagcca
    15661 gaaaaaactg cagtcgctac taacggcggg gaaaaacaaa cgttattaca ccgagacaga
    15721 aggtgcactg cgttatgttg tcgcggacaa cggcgaaaag gggctgacct tcgctgttga
    15781 accgattaag ctggcgctat ctgaatcgct tgaaggtttg aataaatgac aaaaagcaaa
    15841 gcctttgtgc cgatgaatct ctatactgtt tcacagacct gctgccctgc ggggcggcca
    15901 tcttccttta ttcgcttata agcgtggaga attaaaatgc gacatccttt agtgatgggt
    15961 aactggaaac tgaacggcag ccgccacatg gttcacgagc tggtttctaa cctgcgtaaa
    16021 gagctggcag gtgttgctgg ctgtgcggtt gcaatcgcac caccggaaat gtacatcgat
    16081 atggcgaagc gcgaagctga aggcagccac atcatgctgg gtgcgcaaaa cgtggacctg
    16141 aacctgtccg gcgcattcac cggtgaaact tctgccgcta tgctgaaaga catcggcgct
    16201 cagtacatca tcatcggcca ctctgaacgt cgtacttacc acaaagagtc tgacgaactg
    16261 attgcgaaaa aattcgcggt gctgaaagag cagggcctga ctccggttct gtgcatcggt
    16321 gaaaccgaag ctgaaaacga agcgggcaaa actgaagaag tttgcgcacg tcagatcgac
    16381 gcggtactga aaactcaggg tgctgcggca ttcgaaggtg cggttatcgc ttacgaacct
    16441 gtatgggcaa tcggtactgg caaatctgca actccggctc aggcacaggc tgttcacaaa
    16501 ttcatccgtg accacatcgc taaagttgac gctaacatcg ctgaacaagt gatcattcag
    16561 tacggcggct ctgtaaacgc gtctaacgct gcagaactgt ttgctcagcc agatatcgac
    16621 ggcgcgctgg ttggcggtgc ttctctgaaa gctgacgctt tcgcagtgat cgttaaagct
    16681 gcagaagcgg ctaaacaggc ttaagtctga caggtgccgg atttcatatc cggcacttac
    16741 tttccttaac tcctcgcctt aacgcaaaat ctcacactca tgatcctgga tttcctcggc
    16801 tgaagcacgg ttcagcgtca gtagatttcg ttgtgtcgcc agcaacacaa acgaattatc
    16861 actctgccgt accatcgcca gcccgtagct tcccatatgt tcccgcgcct caggcacttc
    16921 ttctgcaagc atcataaatg ggctgcgttg taccagttcg ctttccgtta cccgacgcgc
    16981 caggtattca tgcccgcgca aaccacctgg cagtggcaac cagcggctgc tgatgttcgc
    17041 cagattttta tccagttgtt cgcgcacatc aggacgaata caagagatat gaatatgaaa
    17101 atggttttgc gtacgcccgg tgcgggagtt gatcgccaaa gaaaccgcgc gatcgggaac
    17161 cggctggccg tattttttgc tcataaaatc acgcgcctgc caggccaacc aaaagaagtt
    17221 cggcgttgaa ggatcggtca acaaaggact ttcagtaccg ttaatacgat acgttggcat
    17281 caacagatat tgcagtggac cattaagatc ttttaaaacc acgtatccgg cattgggttt
    17341 gacttccgca catggcgaag gattttgatt ttgctgctga ttgggcaaac attcctcaag
    17401 gacaatctta cgtaatgtat ccgactcttc accggttaat ttccagtaac caataccggc
    17461 agccacaacg gcgataacta tcatcaccaa aaaaagaaga cccgcttttt tcatcttttt
    17521 ttccctgtac ctcaaagaga tgcaagggta acgcaaaatc gtgacaaata aaaaacccgg
    17581 tcgcgagaaa ccaccgggtt tgataattat cctggggaaa tcagcgtttg ctgatctgat
    17641 cgaacgtacc gccgttagca aaatgctctt tttgcgcttt cgtccagccg ccgaactctt
    17701 catcaatggt gaataacttc agctttggaa acgcattttc gtactttttc gctacttcag
    17761 cgtcgcgcgg acggtagtag tttttcgcgg caatttcctg accttctggc gagtagagat
    17821 atttcaggta ggcttccgcc acctctttgg tgcctttttt ctcgaccact ttatcgacca
    17881 ccgacacggt tggctctgcg aggatagact cactcggcgt gacgatttcg aatttatctt
    17941 tccccagttc attcgctacc agcagagctt cgttttccca ggcgatcagt acatcgccaa
    18001 tcccgcgctc gacaaaggtg ttagtggagc cgcgcgcgcc agaatccaga acttcgacgt
    18061 ttttatacag tgcccgaaca aaatcctgtg cttttgcctg atcgttgttg ttgtgatgca
    18121 gcgcgtagcc ccaggcagcc aggtagttcc agcgtgcgcc accagagctt ttcggattag
    18181 gcgtgatcac cgaaacaccc ggtttaatca gatcgttcca gtcatggatc tgcttcggat
    18241 tgcccttacg caccaggaaa acaatggtgg aagtgtacgg tgcggagtta tccggcagac
    18301 gtttgatcca ctctttatca atccgcccgc gttccgcaat cgcgtccacg tcataggcca
    18361 gagccagcgt gacaacatca gcttcaatac cgttgattac cgacgtcgct tgtttacctg
    18421 aaccaccgtg tgactgacga atcaccacgt tatcaccagt ttgctgtttc cagtgggcgc
    18481 tgaatgcctt gttgtactgt tcgtacaatt cgcgcgttgg atcatatgaa acgttaagaa
    18541 gctgaatatc ctttgccata acgttggttg ccgccagcaa aaatgttaac cctacgcccc
    18601 acttgttcat cgcccgactc tcttatgttg tgttgtgatg agcaaagcgt gccagaaggt
    18661 taaccaaaca ttaaagaata aaaaaagatt ggctataact tgcgggtata tgttgggggt
    18721 taaaaaggcg gaagaaatcc gcctcatatt gctgacaaag tgcgctttgt ccatgccgga
    18781 tgcggcgcga acgccttatc cggcctacaa aagtttgcaa attcaataaa ttgcagaatt
    18841 catgtaggcc tgataagcga agcgcatcag gcatttttgc ttctgtcatc ggtttcaggg
    18901 taaaggaatc tgcctttttc cgaaatcatt aatacagttt tttcgcgcag tccagccagt
    18961 cacctttgaa cggacgcttc atgttttcga tagcgtcgat gatgtcgtgg tgaaccagct
    19021 gttcgttctg gatacctaca caacgaccgc cgtaacctgc cagcagcaga tcgatagcgt
    19081 aagcgcccat acgggaagcc agaatacggt cgtaaggcac cggagaacca ccgcgctgga
    19141 tgtggcccag cacagttgcg cgggtttcac gaccggtttc tttctcgatg aaatgcgcca
    19201 gttcgtcaac atcacacata tgttcggtaa tcgccacgat cgcgtgtttt ttacctttcg
    19261 cgatacccgc tttgatttcg tttaccaggt cttcacggct gaattcaact tccggaacca
    19321 caacgaattc acagccaccg gcaatggccg cagccaacgt cagatcgcca caataacggc
    19381 ccatcacttc caccacggaa atacgctggt gagaagaaga ggtgtcacgc agacggtcga
    19441 tcgcttctac aacggtgctc agcgcagtga agaaaccgat agtgtagtca gtgcctttga
    19501 tgtcgttgtc gatagtgccc ggcagaccga tgcacgggaa gcccatttcg gtcagacgca
    19561 ttgcacccat gtaggaaccg tcaccgccga taaccaccag cgcgtcgatc ccacgttttt
    19621 tcaggttttc gatagccacg gcgcggatgt tctcgtcgcg gaattccggg aagcgcgcag
    19681 aaccgaggaa cgtaccgcca cggttgatca tgtcagaaac gctgtaacgg tctagctgta
    19741 ccatacggtc ttcatacaga cccagatagc cgtcataaat gcccattact tccagacctt
    19801 ctgtcagcgc agaacgaaca accccgcgaa ttgcggcgtt catgcctggc gcatcaccgc
    19861 cgcttgtcaa cacaccgatt ttcttaatca tgactacctc tgaactttgg aatgcaaaat
    19921 aaaatctgtt gccggaagtc ttcttgcaca tcgaagtgat ccaacgaatg tgcaaatagt
    19981 ataacaatca cttcctgctg aattgattca ggtcaggcca aatggcggta ttttatacac
    20041 aaaatgcggg tctggctctc ttttatactg attatgaaag catagaccgt ttaccctccc
    20101 tgggtacgac ggaacagggg tcctgatgga taattacatc cgatcccgga aaacgccgta
    20161 aaatagcctg ctctacctga tccgccacca tatgtgcctg aaccaaaggc agagagtctt
    20221 ccatttccaa atgaatctga ataaagcggg tcggccctga ctgccgcgtg cgaagatcgt
    20281 gagcgccgct aacacccggc caggaagtca cgatatcaat aatttcttgc cgttcctcat
    20341 caggcaatgc gcgatccagt aatgactgta ccgcctcata tcccatgcgt aacgcgctat
    20401 ataaaatata gatgccgatt cccaatgcaa acagagcatc ggcgcgatgc cagccgtacc
    20461 aggacaaccc cagcgccagc agaattgcgc cgttcatcat aacatcagac tggtaatgta
    20521 gcatatcagc ccgcaccgcc tggctttgcg tccggcgcac cacccaacgc tgaaacgaga
    20581 caaggataat cgtacaaatt agcgccacaa ttgtcacgat aaccccgacg cctggatctg
    20641 tcatcggtgt tggagatatc agatgttgaa tacccgtcaa aaacaggaat agtgccgaac
    20701 cggagataaa catactttgc gccagcgccg cgagggactc tgctttaccg tgaccaaacg
    20761 agtgattatc gtcggcaggt tgcagggaat atcgcaccac cagtaaattc gtcaacgacg
    20821 cgccgatatc caccagcgaa tccaccagcg cggcgagaat actcaccgac ccggtatacc
    20881 accatgcaaa aattttaatc agcaatagca acgaagccat cgccgtcgca gcaatcgccg
    20941 cccgactgac cagccgtcca taagattgat tcataaatac tcccgctatc aactgacgct
    21001 agtataacgg aagcaaatca tctgcaatgc attaagcagc aggcaaattg aggataaaaa
    21061 aaacccccac atcatgtggg ggaagacagg gatggtgtct atggcaagga aaacagggtt
    21121 tactactggg aacgtgagtt gctactactc aatagcttca acgatgaact tttttgccat
    21181 tgcgtcacgt cacgcaactg ctccattcgt tgttgatgtt tctcgtttaa aaccgcttgc
    21241 tgctccggcg ttaacaggcg atacatttgg ttgcggactt ttgccatctc aacctgacga
    21301 gcaatttgct cattcgccat tttttctgcc tgtgcgcgca cagcgttttc atcaaaattt
    21361 tctgcggtga caaggcgatg cattgtctcc agttcgctaa cattaacagg aggctgttcg
    21421 tgccgggcct gttgcataag atctcgcatc tgctgacgct gatgttcggt taaacttatg
    21481 ccgtcgaaca tatggctctg cgtactgcgc tgcgtaagtt cttcacccgg atgccagtta
    21541 tcgcctgaac cgacttcagc agcgtggctt aatgaactga ctgccagcgt tgaggccatg
    21601 acggcagcgg taactatgcg catcatttgc tcccaaaatc tttctgtcgc gattcaacga
    21661 tagagagttt acgattcagg ctgcaaacat gcgtcagggg gtgtaaaaca acgtaaagtc
    21721 atggattagc gacgtctgat gacgtaattt ctgcctcgga ggtatttaaa caatgaataa
    21781 aatcctgtta gttgatgatg accgagagct gacttcccta ttaaaggagc tgctcgagat
    21841 ggaaggcttc aacgtgattg ttgcccacga tggggaacag gcgcttgatc ttctggacga
    21901 cagcattgat ttacttttgc ttgacgtaat gatgccgaag aaaaatggta tcgacacatt
    21961 aaaagcactt cgccagacac accagacgcc tgtcattatg ttgacggcgc gcggcagtga
    22021 acttgatcgc gttctcggcc ttgagctggg cgcagatgac tatctcccga aaccgtttaa
    22081 tgatcgtgag ctggtggcac gtattcgcgc gatcctgcgc cgttcgcact ggagcgagca
    22141 acagcaaaac aacgacaacg gttcaccgac actggaagtt gatgccttag tgctgaatcc
    22201 aggccgtcag gaagccagct tcgacgggca aacgctggag ttaaccggta ctgagtttac
    22261 cctgctctat ttgctggcac agcatctggg tcaggtggtt tcccgtgaac atttaagcca
    22321 ggaagtgttg ggcaaacgcc tgacgccttt cgaccgcgct attgatatgc acatttccaa
    22381 cctgcgtcgt aaactgccgg atcgtaaaga tggtcacccg tggtttaaaa ccttgcgtgg
    22441 tcgcggctat ctgatggttt ctgcttcatg ataggcagct taaccgcgcg catcttcgcc
    22501 atcttctggc tgacgctggc gctggtgttg atgttggttt tgatgttacc caagctcgat
    22561 tcacgccaga tgaccgagct tctggatagc gaacagcgtc aggggctgat gattgagcag
    22621 catgtcgaag cggagctggc gaacgatccg cccaacgatt taatgtggtg gcggcgtctg
    22681 ttccgggcga ttgataagtg ggcaccgcca ggacagcgtt tgttattggt gaccaccgaa
    22741 ggccgcgtga tcggcgctga acgcagcgaa atgcagatca ttcgtaactt tattggtcag
    22801 gccgataacg ccgatcatcc gcagaagaaa aagtatggcc gcgtggaact ggtcggtccg
    22861 ttctccgtgc gtgatggcga agataattac caactttatc tgattcgtcc ggccagcagt
    22921 tctcaatccg atttcattaa cttactgttt gaccgcccgc tattactgct gattgtcacc
    22981 atgttggtca gtacgccgct gctgttgtgg ttggcctgga gtctggcaaa accggcgcgt
    23041 aagctgaaaa acgctgccga tgaagttgcc cagggaaact tacgccagca cccggaactg
    23101 gaagcggggc cacaggaatt ccttgccgca ggtgccagtt ttaaccagat ggtcaccgcg
    23161 ctggagcgca tgatgacctc tcagcagcgt ctgctttctg atatctctca cgagctgcgc
    23221 accccgctga cgcgtctgca actgggtacg gcgttactgc gccgtcgtag cggtgaaagc
    23281 aaggaactcg agcgtattga aaccgaagcg caacgtctgg acagcatgat caacgatctg
    23341 ttggtgatgt cacgtaatca gcaaaaaaac gcgctggtta gcgaaaccat caaagccaac
    23401 cagttgtgga gtgaagtgct ggataacgcg gcgttcgaag ccgagcaaat gggcaagtcg
    23461 ttgacagtta acttcccgcc tgggccgtgg ccgctgtacg gcaatccgaa cgccctggaa
    23521 agtgcgctgg aaaacattgt tcgtaatgct ctgcgttatt cccatacgaa gattgaagtg
    23581 ggctttgcgg tagataaaga cggtatcacc attacggtgg acgacgatgg tcctggcgtt
    23641 agcccggaag atcgcgaaca gattttccgt ccgttctatc ggaccgatga agcacgcgat
    23701 cgtgaatctg gcggtacagg attggggctg gcgattgttg aaaccgccat tcagcagcat
    23761 cgtggctggg tgaaggcaga agacagcccg ctgggcggtt tacggctggt gatttggttg
    23821 ccgctgtata agcggagtta aactccgcat ttgtaggcag gataaggcgt ttacgccgca
    23881 tccggcattt gagcaggatg cctgatgcga cgctgatagc gtcttatcag gcctacactc
    23941 cgggggttat tttccccaca accggcggga aaaatcttcg atcttgccgc tcagtcggcg
    24001 cttttgcatc gtccgcgtcc agcttttcga taacccggcg gcggagagta aacggtgata
    24061 ctgatcgtca tcaaacggca tatgccatgc gatggcggca gcctcctgca cggtcacatc
    24121 gctgacacgg gaaaccaact ccagcggcgc gtccgcagac actttccctg gcgctatcac
    24181 gctatacaac caccccacct tacctgtgtt ttgcatcaac tgcgctatat cgctgatatc
    24241 aaaatggtaa ttgagtttat agcaaggcga gcgcggctgg ctgacctgaa ttaatgcctc
    24301 tccccagcgg aaaatatcgc ccatatgaac attactttcc gtgagaccgt cggttgagag
    24361 attttcgcca aacgcaggcg caacaaacaa ctccgcctgt tccggaaatt cccgcaccca
    24421 gtagagatag tgctcacgag gataatgaca cagcgctctg tctggcccgc cgtgaacctt
    24481 tttctccgcc tgctcgtcac cttccagccc cagctccgtc agcatcaact caccatcaac
    24541 ctggattttg gcaattgcgc tgggtttgcc ttcgggataa gcctgaatct tgcctgtgta
    24601 tacatcaacc ggatatcgca tctgttccct ctccacgaga aatttcccca ttagaccata
    24661 tttattttat gtcaggcgtg ctgttttcgc ggtaagtatc caggcaggaa ctcaatagca
    24721 aaccggacga acgttagttc atccggctgt ggggattaag gaagcaggtt tatttcaccg
    24781 tacctaaaat ttcgattttc gctgctctgg ctttgacttt acgtgaccag atagaggtaa
    24841 gaattggcac cagaatcgaa gtcacaatga ccgccgtcgc taccagcgaa gttgctgccg
    24901 gagccatcgg tttaaacgca ggcaccattt ctgcaatcag cacaggtgtc gctaccgcgg
    24961 cccctgcgga actggaagcg gcaattccgg cagtgccatc gccaccgcca atcaatttat
    25021 cggcgataat caacggaata ccggtcacga taattactgc cacacccaac aggatcccca
    25081 gcaaaccagt ctgggcaatc acagtcaaat caatggtatt gcccagcgcg aaggcaaaga
    25141 atggaatcag cgtttgcacc gctttgctga aaaattctcg taattcaggg tcaaggttcc
    25201 ccagggcaaa gcccaccagg aacggtaata ctgcgccgac gaaaacatgc ggttcaaacg
    25261 aggcaatccc tgcagtgccc agaataatca tcgtcatgag cggcccggac tccaacgaca
    25321 tcaacacaaa tgccccagct tcttcttttg tgccgtactg ctgcatgatg gaagcgtaaa
    25381 gtccgccatt ggtcatatcc atcgccgcca ccagcgccag cgttgaaagt ccggcaaaga
    25441 atccaacttc aacaccatgt tccggaataa tgcgcgaggc aattgccgca accacccacg
    25501 cgacggcaat ttttgttacc accagcgtac cggatttacg caataccgtt cccgtcgcgc
    25561 ttaattttat tgacgccccc atgcaaaaaa accacaccgc cagaatgggc accgtaccgg
    25621 taatcatccc gttggtgaat gatccaaaat atttccccgc ccccggcgag aaggtgtggc
    25681 acagtgcgcc aaggaatagc gggacgagca tcatcccccc cgggattttc tcaatcgagc
    25741 gttttatctg catttccatc acctgtcggc atatttttaa gggctgatgc gtcattcctc
    25801 aatggagcat cagtgttgat ggaaacgaag atagacattc atttaatcaa taaaagtgat
    25861 caaaaccaca tcaattgaaa cgctgtttta tttttatcgg atcattgttt ttattttgaa
    25921 attgatcaca aaaaaaccac cgccgttggc gatggttcat tttttaagct gatatgcggc
    25981 ctatacgcct cattgcagca ggcggcaaat gattattttt tcgccgcaaa acgtgccgct
    26041 gcttcgtccc agttcaccac gttccagaac tctttaatgt agtccggacg gcggttctgg
    26101 aatttcaggt agtaagcatg ttcccacaca tccaggccca gaatcgggaa gccggaagcg
    26161 ccagaaatag cttcacccat cagcggagaa tcctggttag cagtagaaac caccgccagt
    26221 ttatcgcctt tcagcaccag ccatgcccag ccggaaccaa agcgggaagc tgccgctttt
    26281 tcaaattctg ctttgaagtt atcaacggag ccgaagtcac gttcgatagc cgctttcagg
    26341 tcaccctgca gggtggtgcc ttttttcaga cctttccaga acaggctgtg gttagcgtga
    26401 ccgccagcgt tgttgcgcag tacggttttc ttgtctgctg gcagctggtc cagtttggtg
    26461 atcagctctt caaccggcag gttggcaaat tctggcaggc tttccagcgc cgcgttggcg
    26521 ttgtttacgt aggtctgatg gtgtttggtg tggtggattt ccatggtctg cttatcgaag
    26581 tgcggttcca gggcatcgta agcatacggc agggatggca gggtatagct catattcatc
    26641 tccagtattg tcgggcggcc gattgttaat gccgcgtaag cagttggttc attatagtta
    26701 attaaatgat attgaaaatg attatcaatg ccgtactttt cgtaagggta tggttttgca
    26761 ggaaaatgcc cgagatgtga agcaaatcac ccacttaatg ccgtgattgc cagtaaatcg
    26821 acaacggcgg caacaggcga aaggttaatc gacagcacga tttttacact catctcgtcg
    26881 gagatgtgaa gcgacgaaaa atgatgagga taagaagatg agtaacgcga ttacgatggg
    26941 gatattttgg catttgatcg gcgcggccag tgcagcctgt ttttacgctc cgttcaaaaa
    27001 agtaaaaaaa tggtcatggg aaaccatgtg gtcagtcggt gggattgttt cgtggattat
    27061 tctgccgtgg gccatcagcg ccctgttact accggatttc tgggcgtatt acagctcgtt
    27121 tagtctctct acgctgctgc ctgtttttct gttcggcgct atgtggggga tcggtaatat
    27181 caactacggc ctgaccatgc gttatctcgg catgtcgatg ggaattggca tcgccattgg
    27241 cattacgttg attgtcggta cactgatgac gccaattatc aacggcaatt tcgatgtgct
    27301 gattaacacc gaaggcggac gcatgacgtt gctcggcgtt ctggtggcgc tgattggcgt
    27361 agggattgta actcgagccg ggcagttgaa agagcgcaag atgggcatta aagccgaaga
    27421 gttcaatctg aaaaaagggc tggtgctggc ggttatgtgc ggcattttct ctgccgggat
    27481 gtcctttgcg atgaacgccg caaaaccgat gcatgaagcc gctgccgcac ttggcgtcga
    27541 tccactgtat gtcgctctgc caagctatgt tgtcatcatg ggcggcggcg cgatcattaa
    27601 cctcggtttc tgttttattc gtctggcaaa agtgaaggat ttgtcgctaa aagccgactt
    27661 ctcgctggca aaaccgctga tcattcacaa tgtgttactc tcggcactgg gcgggttgat
    27721 gtggtatctg caattctttt tctatgcctg gggccacgcc cgcattccgg cgcagtatga
    27781 ctacatcagt tggatgctgc atatgagttt ctatgtattg tgcggcggta tcgtcggact
    27841 ggtgctgaaa gagtggagca atgcaggacg ccgtccggta acggtgttga gcctcggttg
    27901 tgtggtgatt attgtcgccg ctaacatcgt cggcatcggc atggcgaatt aatctttctg
    27961 cgaattgaga tgacgccact ggctgggcgt catcccggtt tcccgggtaa acaccaccga
    28021 aaaatagtta ctatcttcaa agccacattc ggtcgaaata tcactgatta acaggcggct
    28081 atgctggaga agatattgcg catgacacac tctgacctgt cgcagatatt gattgatggt
    28141 cattccagtc tgctggcgaa attgctgacg caaaacgcgc tcactgcacg atgcctcatc
    28201 acaaaattta tccagcgcaa agggactttt caggctagcc gccagccggg taatcagctt
    28261 atccagcaac gtttcgctgg atgttggcgg caacgaatca ctggtgtaac gatggcgatt
    28321 cagcaacatc gccaactgcc cgaacagcaa ctctgccatt tcgttagcaa acggcacatg
    28381 ctgactactt tcatgctcaa gctgaccgat aacctgccgc gcctgcgcca tccccacgct
    28441 acctaagcgc cagtgtggtt gccctgcgct ggcgctaaat cccggaatcg ccccctgcca
    28501 gtcaagattc agcttcagcc gctccgggca ataaataata ttctgcaaaa ccagatcgtt
    28561 aacggaagcg taggagtgtt tatcgtcagc atgaatgtaa aagagatcgc cacgggtaat
    28621 gcgataaggg cgatcgttga gtacatgcag gccattaccg cgccagacaa tcaccagctc
    28681 acaaaaatca tgtgtatgtt cagcaaagac atgttgcgga taacggtcag ccacagcgac
    28741 tgcctgctgg tcgctggcaa aaaaatcatc tttgagaagt tttaactgat gcgccaccgt
    28801 ggctacctcg gccagagaac gaagttgatt attcgcaata tggcgtacaa atacgttgag
    28861 aagattcgcg ttattgcaga aaaccatccc gtccctgacg aatatcacgc ggtgaccagt
    28921 taaactctcg gcgaaaaagc gtcgaaaagt ggttactgtc gctgaatccg caatgatagg
    28981 cgatgtcagt aacgctggcc tcactgtggc gtagcagatg tcgggccttc atcagtcgca
    29041 ggcggttcag gtaacgctga ggcgtcagtc ccgtttgctg cttaagctgc cgatgtagcg
    29101 ttcgcagtga aagagaaaat tgatccgcca cggcatccca attcacctca tcggcaaaat
    29161 ggtcctccag ccaggccaga agcaagttga gacgtgatgc gctgttttcc aggttcttct
    29221 gcaaactgct tttacgcagc aagagcagta attgcataaa caaagatctc gcgactggcg
    29281 gtcgagggta aatcattttc cccttcctgc tgttccatct gtgcaaccag ctgtcgcacc
    29341 tgctgcaata cgctgtggtt aacgcgccag tgagacggat actgcccatc cagctcttgt
    29401 ggcagcaact gattcagccc ggcgagaaac tgaaatcgat ccggcgagcg atacagcaca
    29461 ttggtcagac acagattatc ggtatgttca tacagatgcc gatcatgatc gcgtacgaaa
    29521 cagaccgtgc caccggtgat ggtatagggc tgcccattaa acacatgaat acccgtgcca
    29581 tgttcgacaa tcacaatttc atgaaaatca tgatgatgtt caggaaaatc cgcctgaggg
    29641 agccggggtt ctatcgccac ggacgcgtta ccagacggaa aaaaatccac actatgtaat
    29701 acggtcatac tggcctcctg atgtcgtcaa cacggcgaaa tagtaatcac gaggtcaggt
    29761 tcttacctta aattttcgac ggaaaaccac gtaaaaaacg tcgatttttc aagatacagc
    29821 gtgaattttc aggaaatgcg gtgagcatca catcaccaca atgaagcaaa ttgtgaacat
    29881 catcacgttc atctttccct ggttgccaat ggcccatttt cctgtcagta acgagaaggt
    29941 cacgaattca ggcgcttttt agactggtcg taatgaaatt cagcaggatc acattatgac
    30001 ctttcgcaat tgtgtcgccg tcaatctcgg cgcatccagt gggcgcgtga tgctggcgcg
    30061 ttacgagcgt gaatgccgca gcctgacgct gcgcgaaatc catcgtttta aaaatgggct
    30121 gcatagccag aacggttatg tcacctggga tgtggatagc ctggaaagtg ccattcgcct
    30181 tggcttaaac aaagtgtgtg aggaagggat tcgtatcgat agcattggga ttgatacctg
    30241 gggcgtggac tttgtgctgc tcgaccaaca gggtcagcgt gtgggcctgc ccgttgctta
    30301 tcgcgatagc cgcaccaatg gcctaatggc gcaggcacag caacaactcg gcaaacgcga
    30361 tatttatcaa cgtagcggca tccagtttct gcccttcaat acgctttatc agttgcgtgc
    30421 gctgacggag caacaacctg aacttattcc acacattgct cacgctctgc tgatgccgga
    30481 ttacttcagt tatcgcctga ccggcaagat gaactgggag tacaccaatg ccaccaccac
    30541 acaactggtc aatatcaata gcgacgactg ggacgagtcg ctactggcgt ggagcggggc
    30601 caacaaagcc tggtttggtc gcccgacgca tccgggtaat gtcattgggc actggatttg
    30661 cccgcagggt aatgagattc cggtggtcgc cgttgccagc catgataccg ccagcgcggt
    30721 tatcgcctcg ccgttaaacg gttcacgcgc cgcttatctc tcttctggca cctggtcatt
    30781 gatgggcttc gaaagccaga cgccatttac caatgacacg gcgctggcag ccaacatcac
    30841 caatgaaggc ggggcggaag gtcgctatcg ggtgctgaaa aatattatgg gcttatggct
    30901 gcttcagcga gtgcttcagg aacggcaaat caacgatctc ccggcgctta tcgccgcgac
    30961 acaggcactt ccggcctgcc gcttcatcat caatcccaat gacgatcgct ttattaatcc
    31021 tgaggcgatg tgcagcgaaa ttcaggctgc gtgtcgggaa acggcgcaac cgatcccgga
    31081 aagtgatgct gaactggcgc gctgtatttt cgacagtctg gcgctgctgt atgccgatgt
    31141 gttgcatgag ctggcgcagc tgcgcggtga agatttctcg caactgcata ttgtcggcgg
    31201 cggctgccag aacacgctgc tcaaccagct atgcgccgat gcctgcggta ttcgggtgat
    31261 cgccgggcct gttgaagcct caacgctcgg caatatcggc atccagttaa tgacgctgga
    31321 tgaactcaac aatgtggata atttccgtca ggtcgtcagc accaccgcga atctgaccac
    31381 ctttacccct aatcctgaca gtgaaattgc ccactatgtg gcgcagattc actctacacg
    31441 acagacaaag gagctttgcg catgaccact caactggaac aggcctggga gctggcgaaa
    31501 cagcgtttcg cggcggtggg gattgatgtc gaggaggcgc tgcgccaact tgatcgttta
    31561 cccgtttcaa tgcactgctg gcagggcgat gatgtttccg gttttgaaaa cccggaaggt
    31621 tcgctgaccg gtgggattca agctactggt aattatccgg gcaaagcgcg taatgccagt
    31681 gagctacgta ccgatctgga acaggctatg cggctgattc cggggccgaa acggcttaat
    31741 ttacatgcca tctatctgga atcagacacg ccagtctcgc gcgaccagat caaacctgag
    31801 cacttcaaaa actgggttga atgggcgaaa gccaatcagc tcggtctgga ttttaacccc
    31861 tcctgttttt cgcatccgct aagcgccgat ggctttacgc tttcccatgc cgacgacagc
    31921 attcgccagt tctggattga tcactgcaaa gccagccgtc gcgtttcggc ctattttggc
    31981 gagcaactcg gcacaccatc ggtgatgaac atctggatcc cggatggtat gaaagatatc
    32041 accgttgacc gtctcgcccc gcgtcagcgt ctgctggcag cactggatga ggtgatcagc
    32101 gagaagctaa accctgcgca ccatatcgac gccgttgaga gcaaattgtt tggcattggc
    32161 gcggagagct acacggttgg ctccaatgag ttttacctgg ggtatgccac cagccgccag
    32221 acggcgctgt gcctggacgc cgggcacttc cacccgactg aagtgatttc cgacaagatt
    32281 tccgccgcca tgctgtatgt gccgcagttg ctgctgcacg tcagtcgtcc ggttcgctgg
    32341 gacagcgatc acgtagtgct gctggatgat gaaacccagg caattgccag tgagattgtg
    32401 cgtcacgatc tgtttgaccg ggtgcatatc ggccttgact tcttcgatgc ctctatcaac
    32461 cgcattgccg cgtgggtcat tggtacacgc aatatgaaaa aagccctgct gcgtgcgttg
    32521 ctggaaccta ccgccgagct gcgcaagttg gaagcggcgg gcgattacac tgcgcgtcag
    32581 gcactgctgg aagagcagaa atcgttgccg tggcaggcgg tatgggaaat gtattgtcag
    32641 cgtcacgata ccccagcggg tagcgaatgg ctggagagcg tgcgggctta tgagaaagaa
    32701 attttgagtc agcgtgggta aacactgcca gatgcggcac gagcgcctta tccggcctac
    32761 agatcgtgca tctgacaaca ccccatttac aggaacaccg aacatgcaaa acattactca
    32821 gtcctggttt gtccagggaa tgatcaaagc caccaccgac gcctggctga aaggctggga
    32881 cgagcgcaac ggcggcaacc tgacgctacg cctggatgac gctgacatcg caccgtatca
    32941 cgacaatttc caccaacaac cgcgttatat cccgctcagc caacccatgc ctttactggc
    33001 aaatacaccg tttatcgtca ccggctcggg caaattcttc cgtaacgtcc agcttgatcc
    33061 tgcggctaac ttaggcgtcg taaaagtcga cagcgacggc gcgggctacc atattctctg
    33121 ggggttaacc aacgaggccg ttccaacttc tgaacttccg gcacactttc tctcccattg
    33181 cgaacgcatt aaagccacca acggtaaaga tcgggtgatc atgcactgcc atgccaccaa
    33241 cctgatcgcc ctcacctatg tactggaaaa cgacacggcg gtcttcactc gccaactgtg
    33301 ggaaggcagc accgagtgcc tggtggtatt cccggatggc gttggcattc tgccgtggat
    33361 ggtgcccggc acggacgaaa tcggtcaggc gaccgcacaa gaaatgcaaa aacattcgct
    33421 ggtgttgtgg cccttccacg gtgtgttcgg ttccggaccg acactggatg aagcctttgg
    33481 tttgatagat accgcagaga aatcagcgga ggtgttagtc aaaatctact cgatgggcgg
    33541 tatgaagcag accattaccc gtgaggagct gattgcactc ggtgagcgtt ttggcgttac
    33601 gccactggcc agtgcgctgg cactttaata tcccaacaga tatccggcgg tgtgggaaac
    33661 gtcgccggac agatggattt gaacttaagg agagtcatta tgagttttat gttggcatta
    33721 ccgaaaatta gcctgcatgg tgctggggca attggcgata tggtaaatct ggttgcgcat
    33781 aaacagtggg gaaaagcact gatcgttacc gatgggcaac tggttaagtt gggattactg
    33841 gacagcctgt ttatcgcact ggatcaatac gaaatgtctt atcacttatt tgatgaggta
    33901 tttcctaacc ccactgaaga actggtgcaa aaaggctacg ctgcatttca ggatgccgca
    33961 tgtgactaca ttattgcttt cggtggcggt agcccaattg atactgcaaa agccgtaaaa
    34021 atccttaccg ctaatcctgc gccttctacg gcgtactctg gtgttggtaa agtgacaaat
    34081 cccggcgtac cgctggtggc aatcaatacg actgccggaa cagctgctga gatgaccagc
    34141 aatgcggtga tcatcgacac cgcgcggcag gtaaaagagg tcattatcga cccgaatatt
    34201 attcccgata ttgcggtgga tgatgccagt gtaatgctgg agatcccggc ctcggttacc
    34261 gccgccacgg gtatggatgc actgactcac gcagttgaag cttatgtttc tgttggtgca
    34321 catccattaa ccgacgctaa cgcactggaa gctatccgtt taattacgct gtggctgcca
    34381 aaagcggtag atgatggtca taatctggaa gctcgtgaac aaatggcatt cgcccagtac
    34441 ctggcgggta tggcatttaa cagtgctggt ctgggccttg ttcatgcact tgcgcatcag
    34501 cccggcgcca cccacaacct gccgcatggt gtgtgcaatg ctatcttgtt gccagttatt
    34561 gaaaacttta accgcccgaa tgccatcgca cgctttgccc gcattgctca ggcaatgggt
    34621 gtcgatacgc gtgatatgag tgaagaagcg gcaagtactg aagcaattaa cgctatccgc
    34681 gcattaagcc atcgcgtggg tattccggca ggattcagcc agcttggcat caccaaagaa
    34741 gatattgaag gctggttaga taaagccctg gctgacccgt gtgtgccatg taacccgcgt
    34801 accgccagcc gcgacgaggt gcgcgagctg tatctggagg cattatgatc cgtaaggcat
    34861 ttgtgatgca ggttaacccc gacgctcacg aagagtatca acgtcggcat aaccccatct
    34921 ggccggaact ggaagcagtg ttgaaatctc acggtgcgca taactacgcc atttacctcg
    34981 acaaagcgcg taatctgctg tttgccacgg tagagattga atctgaagag cgctggaatg
    35041 cggttgccag tactgatgtt tgccaacgtt ggtggaaata tatgaccgac gtcatgcccg
    35101 cgaacccgga taacagcccg gtgagtagcg aactgcaaga agtgttttac ctgccgtaat
    35161 tgcctaatgt gccggagacg cctccggtac aatattttta atttcgattt gaaaaataaa
    35221 catattacac cttcagttaa tatatctcgg taacttatat ttccccgatg cattgtgagt
    35281 aaatcacaaa aataatgaat aactcattaa tgattcatgt ggtttattta aataaccatt
    35341 atgtgcatta ctccgcaaat ctggcctttc actctgtctc aaattgtcat tgtttaccga
    35401 catatcacct ctcatcactt aatcgctcca ccattacaat tgattaaaat atattttatg
    35461 gagtccgatt aatggcagct cttactgcaa gctgtattga cctgaatatt cagggcaatg
    35521 gcgcttattc cattctgaag cagttggcga caatggcgtt acaaaacggt tttatcaccg
    35581 actcacacca gttcctgcaa accctgctgc tgcgcgaaaa aatgcactct actggatttg
    35641 gttccggcat cgccgtgccg cacggtaaaa gcgcctgcgt taaacaaccg ttcgtgttat
    35701 tcgcccgcaa agcgcaggcc attgactgga aagccagcga cggcgaagac gtcaattgct
    35761 ggatctgcct cggcgtgccg caaagtggcg aagaggatca ggtcaaaatc atcggcacac
    35821 tgtgtcgcaa aattatccat caggatttta tcaaccaact gcgacagggc gacgccgacc
    35881 aggtacttgc tttgttaaat caaaccctca gctcataagg aagtggcgat ggagtcatcc
    35941 ttacgtattg tcgcgatcac caactgcccc gccgggatcg ctcacaccta catggtggcg
    36001 gaagccctgg aacagaaagc ccgttctctc ggccatacca taaaagtgga aactcaaggg
    36061 tccagcggcg ttgaaaaccg cttatccagc gaagagattg ccgctgccga ttacgtcatt
    36121 ctagctaccg ggcgtggcct gagcggtgat gaccgggcgc ggtttgccgg gaagaaagtt
    36181 tatgagattg ccatctccca ggcgctgaaa aatatcgacc agattttcag cgaattacca
    36241 acaaactcgc agctttttgc cgcagatagc ggcgtgaagc tcggcaaaca ggaagtgcaa
    36301 agcggcagcg tgatgagcca cctgatggcg ggcgtctctg cggcgctgcc atttgtcatc
    36361 ggcggcggta ttctggtggc gcttgccaac atgctggtgc agttcggttt gccatatacc
    36421 gatatgtcga aaggtgcgcc gtcgtttacc tgggtagttg aatccatcgg ttacctcggc
    36481 ttcaccttta tgatccccat catgggcgct tatattgcct cgtcgattgc cgataaaccc
    36541 gctttcgctc cggcatttct ggtttgctat ctggcgaacg ataaagccct gctcggcacc
    36601 cagtcaggcg cgggcttcct cggcgcggtg gtgctggggc tggcgattgg ctatttcgtc
    36661 ttctggttcc gtaaagtccg tcttggcaaa gcattacaac cgctgctcgg ctccatgctt
    36721 atcccattcg ttactctgct ggtttttggc gtgctcactt actacgttat cgggccggtg
    36781 atgtccgacc tcatgggcgg gctgctccac ttcctgaata ccattcctcc atcaatgaag
    36841 tttgcggcag cgtttctggt cggtgcgatg ctggcgttcg atatgggcgg ccccatcaat
    36901 aaaaccgcct ggttcttctg cttctcactg ctggaaaaac acatttatga ctggtacgcc
    36961 atcgtcggcg ttgtcgcgct gatgccgccc gtcgcggcgg gtcttgcgac gttcattgcg
    37021 cccaaactgt ttacccgaca ggaaaaagag gctgccagta gtgccattgt ggtcggtgcc
    37081 acagtcgcaa ccgaaccggc tattccttac gctctggcag ctccgttgcc gatgattacc
    37141 gccaatacgc tggcgggcgg tattaccggc gtgctggtga tcgctttcgg aatcaaacgt
    37201 ctggcacctg gtttgggtat ctttgacccg ctgatcggcc tgatgtcacc ggtaggttcg
    37261 ttttatctgg tgctggcgat tggcctcgcg ctgaacattt cattcattat cgtcctgaaa
    37321 ggattgtggt tacgccgcaa agcgaaagcc gcgcaacagg agcttgtcca tgaacattga
    37381 gttactgcaa cagttgtgcg aagccagcgc cgttagcggc gatgaacagg aagttcgcga
    37441 cattctgata aacactctgg aaccttgcgt gaatgaaatc acctttgatg ggctgggcag
    37501 ctttgtcgcc cgtaagggaa ataaagggcc gaaagttgcc gttgtcgggc atatggatga
    37561 agtcggcttt atggtcaccc acatcgacga gagcggtttt ctgcgtttca ccaccattgg
    37621 tggctggtgg aatcagtcga tgctcaacca ccgggtaacc atacgcacac acaagggagt
    37681 gaaaatccct ggtgtgattg gttccgtcgc gcctcatgcg ttaacggaaa agcaaaagca
    37741 acagccgcag tcatttgatg agatgttcat tgatattggc gcaaacagtc gcgatgaggt
    37801 ggaaaaacgc ggtgtggcga tgggcgattt tattagcccg gaagccaatt ttgcctgctg
    37861 gggcgaagat aaagtggtcg gcaaggcgtt ggataaccgc atcggctgcg caatgatggc
    37921 tgagctacta cagacggtga ataatcccga aattacgctg tatggcgttg gcagtgtgga
    37981 agaagaagtt gggctacgcg gggcgcaaac ctcggctaaa cacattaaac cggatgtggt
    38041 gatcgtgctg gacaccgctg tcgcgggcga tgttccaggc attgataaca ttaaatatcc
    38101 gctgaaactg ggcaacgggc cggggttgat gctgtttgac aagcgctact tccccaacca
    38161 gaaactggta gcagcgttaa aaagctgtgc cacgcagaat gatttaccgc tgcaattttc
    38221 caccatgaaa accggtgcga cggatggcgg gcgctacaac gtgatgggcg gtgggcgtcc
    38281 ggttgtcgcg ctgtgtttgc caacgcgtta tctgcacgcc aacagcggca tgatttcaaa
    38341 agccgattac gatgctctgc tcacgctgat acggggtttt ctgaccacct taaccgcgga
    38401 aaaagtcagc gcgtttagcc agttccgtca ggtggattaa tgctaaacga acgccagtta
    38461 aagattgtcg atctgctgga gcaacagccg cgcacgcctg gcgagctggc gcaacagact
    38521 ggcgtttctg gcaggaccat actgcgtgat attgactatc tcaactttac ccttaacggc
    38581 aaagcccgca tttctgccag tggtagtgcg ggctatcagc tggaaatctt cgagcgccgc
    38641 agcttttttc agttgctgca aaagcacgat aacgacgatc ggctgctggc actgttatta
    38701 ctgaatacct ttactccccg tgcgcaactc gcctcggcgc ttaatttgcc ggaaacgtgg
    38761 gtcgcagagc gtctgccccg gttaaaacag cgttatgaac gcacttgctg cctggccagt
    38821 cgccccggtt tgggccattt cattgatgag acagaagaaa aacgcgttat attgctggcg
    38881 aacttgctgc gcaaagatcc gtttttaatt ccgctggcgg gcgtaacacg agacaacctt
    38941 cagcatttat ccacggcctg cgacaaccaa caccgctggc cgctcatgca gggtgattat
    39001 ctctccagcc tgattctggc gatttacgcc ctgcgtaatc aactgaccga tgagtggccg
    39061 caatatcccg gtgacgagat aaaacaaatc gttgaacata gcggtctgtt tcttggtgat
    39121 aacgctgtaa gaaccctgac aggtttgata gagaaacagc atcagcaagc gcagataatt
    39181 tctgccgata atgtgcagcg gttgctgcaa agggtgccgg gcatcgcgtc attgaatatt
    39241 attgatacgc agctggttga gaacattacc gggcatttat tacgttgcct tgccgcaccg
    39301 gtgtggattg ctgagcaccg ccaaagcagc atgaataacc tgaaagccgc ctggcctgcg
    39361 gcctttgata tgagtctgca ctttattacg ctactgcgtg aacagctcga tattcccctt
    39421 ttcgacagcg atctgatcgg tttgtatttt gcctgtgcgc tggaacggca tcaaaacgaa
    39481 cgccagccga tcattttgct ctcggaccag aacgcgattg ccactattaa tcagctcgcc
    39541 attgagcgcg atgttttaca ttgtcgggtg attattgccc gtagcttaag cgaacttgtt
    39601 gccattcgcg aagagattga gccgttattg atcattaaca acagccatta tttactggat
    39661 gacgcggtaa ataattacat caccgtaaaa aatatcatta cggctgccgg tatcgaacaa
    39721 ataaaacatt ttctggcgac ggcatttatt cgccaacagc cggagcgttt tttctctgct
    39781 cccggaagtt ttcattattc gaatgtacgc ggtgaaagct ggcaacatat tacccggcaa
    39841 atttgtgcgc aattagtcgc acaacaccat attaccgccg atgaagcaca acgcatcatc
    39901 gcccgcgaag gcgaaggtga aaacctgatt gttaatcgcc tcgccatccc acattgctgg
    39961 agcgaacagg agcgacgttt tcgtggattt tttattaccc tcgcccaacc agttgaggtg
    40021 aataacgaag tcattaacca tgtcttgatc gcctgcgccg ccgccgatgc gcgtcatgag
    40081 ctaaaaatat ttagctatct ggcaagcata ttgtgtcagc atccggcgga gattattgcc
    40141 gggttaacag gatatgaggc atttatggag ttacttcaca aggggtgaac tgtgtcggat
    40201 gcgccgcatc cgacactgca tttatcatca aaaacgttta acgcaggcta ttatagtcat
    40261 caaccatttc gttgtactcg cgtaatgctc tcgggaaaga gtcttcgacc atccagcttg
    40321 agtttgcgtc ctgaagttgc tctttatccc agtcagagta cgggacttta tctctgatgc
    40381 ggcgaacgta ttttttagcc tcgagttgat actggcctgc cgaattaata aacgagaaat
    40441 tcatgccgcc tttatccgct tctttggcct gcgccactag cgtttccagt tcagacactt
    40501 tcttcattgc cgcatcaaca tcaaacttct cttgcgatat caggttatta atttgtttcg
    40561 ctgaaatcat gactgccaga gagtagtagt ggaatgtttt cccttctctt tcttcaatat
    40621 ttttcagttc cgcaagctgg cgcttatcgt taatttcctg aatcgccgca tgataagatt
    40681 ctgctacagg ttcaaaggct tccagatttt ttaagaatgt ctggtgcagc gttttccctt
    40741 tcgcaaacgc atcgtcttta taattttcct gggtgtaata tttatccatt tcgttaatgg
    40801 tattacccaa cgccacggca gcatcgatat aactcaccgc cacgccatca attggttgca
    40861 gcgcaggcgt taacgccaca gcacttttta cgcctttttc acactcagcg aggttggatt
    40921 cactaatgcc gtagatgcca taaacagtac gctcttcacc ggtaggcccc tgtttaaaat
    40981 ctttcagcca gtcagcataa cgcgccagac tgctctgtac agggatctgt aacttgttat
    41041 agcacttgat atagacatcc attttttcgt cgaaaaccgc ttgttcatcg accaccggtt
    41101 gcgcaggtgt ttcaggttta gcttctgctt ttgcaacggg agcttcagga gctggtgcgg
    41161 caggttgact attggcaggc gggggcgttt ctgtttcggc ttttttatca tcacaacccg
    41221 tcagaccgag ggccatgatg gcgactataa tagcggaaga taataaattc cttttcattt
    41281 aattgcctta taaatatata gataagagag tgaacataat aaatgaaa
//
LOCUS       DAZGLJ010000017        40760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000017 DAZGLJ010000000
VERSION     DAZGLJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.017"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..106
                     /locus_tag="ACHK49_000833"
     CDS             <1..106
                     /locus_tag="ACHK49_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO9261521.1"
                     /translation="TTMTITTPTGEGVTSARTFVRLRRCVLVDAFRIV"
     gene            117..953
                     /gene="eutJ"
                     /locus_tag="ACHK49_000834"
     CDS             117..953
                     /gene="eutJ"
                     /locus_tag="ACHK49_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO9261522.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGLRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            943..2130
                     /gene="eutG"
                     /locus_tag="ACHK49_000835"
     CDS             943..2130
                     /gene="eutG"
                     /locus_tag="ACHK49_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO9261523.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQSRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            2247..3473
                     /gene="eutH"
                     /locus_tag="ACHK49_000836"
     CDS             2247..3473
                     /gene="eutH"
                     /locus_tag="ACHK49_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO9261524.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            3470..4873
                     /gene="eutA"
                     /locus_tag="ACHK49_000837"
     CDS             3470..4873
                     /gene="eutA"
                     /locus_tag="ACHK49_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO9261525.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLMETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            4885..6246
                     /gene="eutB"
                     /locus_tag="ACHK49_000838"
     CDS             4885..6246
                     /gene="eutB"
                     /locus_tag="ACHK49_000838"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO9261526.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            6267..7154
                     /gene="eutC"
                     /locus_tag="ACHK49_000839"
     CDS             6267..7154
                     /gene="eutC"
                     /locus_tag="ACHK49_000839"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO9261527.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            7164..7823
                     /gene="eutL"
                     /locus_tag="ACHK49_000840"
     CDS             7164..7823
                     /gene="eutL"
                     /locus_tag="ACHK49_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO9261528.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            7836..8336
                     /gene="eutK"
                     /locus_tag="ACHK49_000841"
     CDS             7836..8336
                     /gene="eutK"
                     /locus_tag="ACHK49_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO9261529.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            8382..9434
                     /gene="eutR"
                     /locus_tag="ACHK49_000842"
     CDS             8382..9434
                     /gene="eutR"
                     /locus_tag="ACHK49_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO9261530.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHCEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(9440..10339)
                     /gene="hemF"
                     /locus_tag="ACHK49_000843"
     CDS             complement(9440..10339)
                     /gene="hemF"
                     /locus_tag="ACHK49_000843"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO9261531.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERECNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(10343..11212)
                     /gene="amiA"
                     /locus_tag="ACHK49_000844"
     CDS             complement(10343..11212)
                     /gene="amiA"
                     /locus_tag="ACHK49_000844"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO9261532.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            11426..11851
                     /gene="ypeA"
                     /locus_tag="ACHK49_000845"
     CDS             11426..11851
                     /gene="ypeA"
                     /locus_tag="ACHK49_000845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO9261533.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            11838..12287
                     /gene="yfeZ"
                     /locus_tag="ACHK49_000846"
     CDS             11838..12287
                     /gene="yfeZ"
                     /locus_tag="ACHK49_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO9261534.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            12348..12923
                     /gene="yfeY"
                     /locus_tag="ACHK49_000847"
     CDS             12348..12923
                     /gene="yfeY"
                     /locus_tag="ACHK49_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO9261535.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            13019..13918
                     /gene="yfeX"
                     /locus_tag="ACHK49_000848"
     CDS             13019..13918
                     /gene="yfeX"
                     /locus_tag="ACHK49_000848"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO9261536.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(13976..15280)
                     /gene="pbp4b"
                     /locus_tag="ACHK49_000849"
     CDS             complement(13976..15280)
                     /gene="pbp4b"
                     /locus_tag="ACHK49_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO9261537.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(15285..16709)
                     /gene="murP"
                     /locus_tag="ACHK49_000850"
     CDS             complement(15285..16709)
                     /gene="murP"
                     /locus_tag="ACHK49_000850"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO9261538.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(16713..17609)
                     /gene="murQ"
                     /locus_tag="ACHK49_000851"
     CDS             complement(16713..17609)
                     /gene="murQ"
                     /locus_tag="ACHK49_000851"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO9261539.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            17773..18630
                     /gene="murR"
                     /locus_tag="ACHK49_000852"
     CDS             17773..18630
                     /gene="murR"
                     /locus_tag="ACHK49_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO9261540.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            18759..19550
                     /gene="ucpA"
                     /locus_tag="ACHK49_000853"
     CDS             18759..19550
                     /gene="ucpA"
                     /locus_tag="ACHK49_000853"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO9261541.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTDMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            19721..20737
                     /gene="cysP"
                     /locus_tag="ACHK49_000854"
     CDS             19721..20737
                     /gene="cysP"
                     /locus_tag="ACHK49_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO9261542.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            20737..21570
                     /gene="cysT"
                     /locus_tag="ACHK49_000855"
     CDS             20737..21570
                     /gene="cysT"
                     /locus_tag="ACHK49_000855"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO9261543.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            21570..22445
                     /gene="cysW"
                     /locus_tag="ACHK49_000856"
     CDS             21570..22445
                     /gene="cysW"
                     /locus_tag="ACHK49_000856"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO9261544.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQDKRAQQEEHHEH"
     gene            22435..23532
                     /gene="cysA"
                     /locus_tag="ACHK49_000857"
     CDS             22435..23532
                     /gene="cysA"
                     /locus_tag="ACHK49_000857"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO9261545.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            23666..24577
                     /gene="cysM"
                     /locus_tag="ACHK49_000858"
     CDS             23666..24577
                     /gene="cysM"
                     /locus_tag="ACHK49_000858"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO9261546.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(24580..24948)
                     /gene="yfeK"
                     /locus_tag="ACHK49_000859"
     CDS             complement(24580..24948)
                     /gene="yfeK"
                     /locus_tag="ACHK49_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO9261547.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            25053..25904
                     /gene="pdxK"
                     /locus_tag="ACHK49_000860"
                     /gene_synonym="thiJ"
     CDS             25053..25904
                     /gene="pdxK"
                     /locus_tag="ACHK49_000860"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO9261548.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(25946..26455)
                     /gene="crr"
                     /locus_tag="ACHK49_000861"
     CDS             complement(25946..26455)
                     /gene="crr"
                     /locus_tag="ACHK49_000861"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO9261549.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(26496..28223)
                     /gene="ptsI"
                     /locus_tag="ACHK49_000862"
     CDS             complement(26496..28223)
                     /gene="ptsI"
                     /locus_tag="ACHK49_000862"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO9261550.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(28268..28525)
                     /gene="ptsH"
                     /locus_tag="ACHK49_000863"
     CDS             complement(28268..28525)
                     /gene="ptsH"
                     /locus_tag="ACHK49_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO9261551.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(28909..29880)
                     /gene="cysK"
                     /locus_tag="ACHK49_000864"
     CDS             complement(28909..29880)
                     /gene="cysK"
                     /locus_tag="ACHK49_000864"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO9261552.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(30065..30826)
                     /gene="cysZ"
                     /locus_tag="ACHK49_000865"
     CDS             complement(30065..30826)
                     /gene="cysZ"
                     /locus_tag="ACHK49_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO9261553.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            31056..32042
                     /gene="zipA"
                     /locus_tag="ACHK49_000866"
     CDS             31056..32042
                     /gene="zipA"
                     /locus_tag="ACHK49_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO9261554.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            32113..34128
                     /gene="ligA"
                     /locus_tag="ACHK49_000867"
     CDS             32113..34128
                     /gene="ligA"
                     /locus_tag="ACHK49_000867"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO9261555.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            34130..34348
                     /gene="ypeB"
                     /locus_tag="ACHK49_000868"
     CDS             34130..34348
                     /gene="ypeB"
                     /locus_tag="ACHK49_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO9261556.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(34345..35343)
                     /gene="yfeH"
                     /locus_tag="ACHK49_000869"
     CDS             complement(34345..35343)
                     /gene="yfeH"
                     /locus_tag="ACHK49_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO9261557.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            35433..36359
                     /gene="yfeR"
                     /locus_tag="ACHK49_000870"
     CDS             35433..36359
                     /gene="yfeR"
                     /locus_tag="ACHK49_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9261558.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(36398..37162)
                     /gene="yfeN"
                     /locus_tag="ACHK49_000871"
     CDS             complement(36398..37162)
                     /gene="yfeN"
                     /locus_tag="ACHK49_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO9261559.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            37411..38244
                     /gene="xapA"
                     /locus_tag="ACHK49_000872"
     CDS             37411..38244
                     /gene="xapA"
                     /locus_tag="ACHK49_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFO9261560.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            38304..39560
                     /gene="xapB"
                     /locus_tag="ACHK49_000873"
     CDS             38304..39560
                     /gene="xapB"
                     /locus_tag="ACHK49_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO9261561.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            39613..39771
                     /locus_tag="ACHK49_000874"
     CDS             39613..39771
                     /locus_tag="ACHK49_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFO9261562.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            39812..40696
                     /gene="xapR"
                     /locus_tag="ACHK49_000875"
     CDS             39812..40696
                     /gene="xapR"
                     /locus_tag="ACHK49_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO9261563.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
BASE COUNT         9509 a        10257 c        11287 g         9707 t
ORIGIN      
        1 gaccaccatg accatcacca cgccaaccgg tgaaggggta accagcgcgc ggacgtttgt
       61 ccgtctgcgt cgctgtgtat tagtcgatgc gtttcgcatt gtttaaggag taaccgatgg
      121 cgcacgacga acaatggctc accccacgtc tgcaaacggc ggcaacgctg tgtaaccaga
      181 cgcctgccgc gacggaatct ccgctgtggc tgggggtgga tctgggcacc tgcgatgtgg
      241 tgtcgatggt tgttgaccgc gacggtcagc cggtggcggt atgcctcgac tgggccgatg
      301 tggtccgcga cggcatcgtc tgggatttct tcggtgcggt caccattgtt cgtcgccatc
      361 tcgacactct tgagcagcaa ttcggtcttc gttttagcca tgcggcgact tcatttcctc
      421 caggtacgga cccgcgtatt tccatcaacg tgctggaatc tgccgggctg gaagtgagcc
      481 atgtgctgga tgagccaacc gcagtcgcgg atctgctgca actggacaac gccggtgtag
      541 tggatatcgg cggcggcact accggcattg ccatcgtgaa aaagggcaag gtgacgtact
      601 cggcggatga agccaccggc ggacatcaca tctctctgac tctcgccgga aatcgtcgta
      661 tttcgctgga agaggcagag cagtacaagc gcggtcatgg tgacgagatt tggcccgcgg
      721 tgaaaccggt gtacgaaaaa atggcggaca ttgtcgcccg gcatattgaa gggcagggca
      781 ttactgattt atggctggcg ggcggctctt gtatgcaacc gggcgtggcg gaactgtttc
      841 gcaaacaatt cccggcgtta caggtgcatt taccgcagca cagtttgttt atgaccccgc
      901 tggcgatcgc cagtagtggg cgagagaaag cggaggggct atatgcaaaa tgaattgcag
      961 accgcgctct ttcaggcgtt cgataccctg aatctgcaac gggtaaaaac atttagcgtt
     1021 ccaccggtga cgctttgcgg tccgggctcg gtgagcagtt gcggacagca agcgcaaacg
     1081 cgtgggctga aacatctgtt cgtgatggca gacagctttt tgcatcaggc agggatgacc
     1141 gccgggctga cgcgtagcct ggccgttaaa ggtatcgcca tgacgctctg gccatgtccg
     1201 gtgggcgaac cgtgcatcac cgacgtgtgt gcagccgtgg cgcagttgcg tgagtcaggc
     1261 tgtgatgggg tgatcgcatt tggcggcggc tcggtgctgg atgcggcgaa agccgtggcg
     1321 ttgctggtga cgaacccgga tagcacgctg gcagagatgt cagaaaccag cgttctgcaa
     1381 tcgcgcctgc cgttgattgc cattcccact accgccggaa ccggctctga aaccaccaac
     1441 gtaacggtga ttatcgatgc ggtgagcggg cgtaagcagg tgttagccca tgcctcgctg
     1501 atgccggatg tggcgattct cgacgccgca ttgaccgaag gtgtgccgtc gcatgtcacg
     1561 gcgatgaccg gcattgatgc gttaacccat gccattgaag catacagcgc cctgaacgct
     1621 acaccgttta ccgacagtct ggcgattggt gccattgcga tgattggcaa atcgctgccg
     1681 aaagcggtgg gctacggtca tgaccttgcc gcgcgcgaga gcatgttact ggcttcatgt
     1741 atggcgggaa tggcgttttc cagtgcgggg cttgggttgt gccacgcgat ggcgcatcag
     1801 ccaggggcgg cgctgcatat tccacacggt ctggcgaacg ccatgttgct gccaacggtg
     1861 atggaattta accggatggt ttgtcgcgaa cggtttagtc agattggtcg ggcactgcga
     1921 actaaaaaat ccgacgatcg tgacgctatt aacgcggtaa gtgagctgat tgcggaagtt
     1981 gggattggta aacgactggg cgatgttggc gcgacatctg cgcattacgg tgcatgggcg
     2041 caggccgcgc aggaagatat ttgtctgcgc agcaacccgc gtaccgccag cctggagcag
     2101 attgtcggcc tgtacgcagc ggcgcaataa atgccggatg cgacgcttgc cgcgtctgat
     2161 ccggccttcg ggattgcaca tgtagggcgg ataaggcgtt tacgccgcat ccgccaataa
     2221 ataacaacta acagggagta aaggcgatgg gaattaacga aatcatcatg tacatcatga
     2281 tgttctttat gctgatagct gccgtagaca ggatcctgtc gcagttcggc ggttctgctc
     2341 gtttcctcgg taagttcggt aaaagtatcg aaggatcagg cggtcagttc gaagaaggct
     2401 ttatggcaat gggcgcactg ggcctggcga tggtcggtat gaccgcgctg gcaccggtac
     2461 tggctcacgt actcgggccg gtaattattc cggtttacga aatgctcggc gctaacccat
     2521 cgatgttcgc cggaacactg ctggcgtgcg atatgggcgg cttcttcctc gccaaagagc
     2581 tggcgggcgg cgacgtagca gcgtggctat actctgggtt aattctcggg tcgatgatgg
     2641 ggccaacgat tgtgttttcc attccggtgg cgctcggcat tatcgaacct tctgaccgtc
     2701 gttatctggc gctcggcgtg ctggcgggca ttgtgaccat tccgattggc tgtattgctg
     2761 gtggtctggt tgctatgtac tccggtgtgc agatcaacgg tcagccggtg gaattcactt
     2821 tcgccctgat cctgatgaac atgatcccgg tgatcattgt cgcgattctg gtggcgctgg
     2881 ggctgaaatt catcccggaa aaaatgatca acggcttcca gatcttcgcc aaattcctcg
     2941 tggcattgat caccctcggt cttgccgctg cggtagtgaa attcctcctt ggctgggaac
     3001 tgatccccgg actggatcct atctttatgg cccctggcga taaacccggt gaagtgatgc
     3061 gcgccattga agttatcggt tctatctcct gcgtgctgtt aggggcgtat ccaatggtgc
     3121 tgctgctgac tcgctggttt gaaaaaccgc tgatgagcgt cggtaaagtg ctgaatatga
     3181 acaacatcgc ggcagccggc atggtggcaa cgcttgccaa caacatcccg atgttcggca
     3241 tgatgaagca gatggatacc cgcggcaaag tcatcaactg cgccttcgcc gtttccgctg
     3301 ctttcgccct gggcgaccac ttaggcttcg ccgctgccaa catgaacgcc atgatcttcc
     3361 cgatgattgt cggcaagttg atcggcggcg taacggcgat tggcgtggcg atgatgctgg
     3421 tgccaaaaga agacgcgacc gcgactaaaa ccgaagcgga ggcacaatcg tgaacactcg
     3481 ccagctattg agcgtcggta tcgatatcgg caccaccacc acccaggtga ttttctccca
     3541 cctggagctg gttaaccgtg cggcggtgtc gcaggtgccg cgctacgaat tcattaaacg
     3601 cgaaattagc tggcaaagtc cggtgttctt tacccctgtc gataaacagg gcggtttaaa
     3661 agaagcggaa ctgaaaacct taatactcga gcaatatcag gctgcgggta ttgcgccgga
     3721 aagcgttgat tctggtgcca tcatcatcac cggtgaaagc gcgaaaaccc gcaatgctcg
     3781 cccggcggtg atggcgctct ctcaatcgct gggggatttt gtcgttgcca gcgccgggcc
     3841 gcacctcgaa tccgtgatcg ccggtcacgg agctggggcg caaacccttt ctgaacaacg
     3901 gctgtgtcgg gtactgaata tcgacatcgg cggtggcacc gcgaactacg ccctgttcga
     3961 tgccggaaaa atcagcggca ctgcctgtct caacgtcggt ggtcgcctga tggaaaccga
     4021 cagccagggg cgcgtggttt acgctcataa accggggcag atgattgtgg atgagtgctt
     4081 cggtgcaggc actgacgccc gttcgctgac cggcgcgcag ctggtgcagg ttacccggcg
     4141 gatggcggcg ctgattgtcg aagtgattga cggaacgctt tcgccgctcg cgcaggcatt
     4201 gatgcaaacc ggtttgctgc ccgcaggtgt tacgcccgaa atcattacgc tttctggagg
     4261 cgtgggcgaa tgttatcgcc accagcccgc cgacccgttc tgttttgccg atattggccc
     4321 acttctggca acggcgctgc atgaccatcc gcgcctgcgt gagatgaatg tgcagtttcc
     4381 ggcgcaaacc gtacgcgcca cggtgattgg cgcgggtgca catacccttt cgctctctgg
     4441 cagcacaatc tggctggagg gcgtacaact gccgctgcgc aatttgccgg tggcgatccc
     4501 gattgatgaa acggatctgg tgagtgcctg gcaacaggcg ctgcttcagc tggatcttga
     4561 tcccaaaact gacgcgtacg tgctggcgct tcccgcctcg ctgcctgtgc gttacgccgc
     4621 ggtactgacg gtcatcaacg cgctggtcga tttcgtcgcg cgttttccga atccgcatcc
     4681 cctgctggtg gtggccgggc aggactttgg taaagctctg ggcatgttgt tgcgcccaca
     4741 gctacaacaa ctcccgttgg cagtcattga cgaagtgatt gtccgcgcgg gggactatat
     4801 cgacattggt acgcctcttt ttggcggatc ggttgtgccg gtgacggtga aatcactcgc
     4861 atttccttcc tgagggaacg acttatgaaa ctaaagacca cattgttcgg caatgtatat
     4921 cagtttaagg atgtaaaaga ggtgctggct aaagccaacg aactgcgttc gggggatgtg
     4981 ctggcgggcg ttgcagcggc aagctcacag gagcgcgtgg cggcaaagca ggtgttgtcg
     5041 gaaatgaccg tagcggacat ccgcaataat ccggtgattg cctatgaaga tgactgcgtg
     5101 acgcggctga ttcaggacga tgttaacgaa acggcctaca accagattaa aaactggagc
     5161 atcagcgaac tgcgtgagta tgtgctgagc gatgaaacca gcgtggacga cattgccttt
     5221 acccgcaaag ggctgacctc ggaagtggtc gcggcggtag cgaagatttg ctccaacgcg
     5281 gacctgatct acggcgcgaa gaaaatgccg gtaatcaaaa aggccaatac caccattggt
     5341 attccgggca ccttcagcgc ccgtttgcag ccgaacgata cccgtgacga cgtgcaaagt
     5401 atcgctgcgc aaatctatga agggctttcc tttggggtgg gcgatgcggt gatcggcgtt
     5461 aacccggtaa ctgacgacgt ggaaaactta agccgcgtgt tggacaccat ttacggcgtg
     5521 atcgacaaat tcaacatccc aactcagggc tgcgtactgg cgcacgtcac cacccagatc
     5581 gaagcgattc gtcgcggcgc gcctggcgga ctgattttcc agagtatctg tggcagcgaa
     5641 aaagggctga aagagtttgg cgtggaactg gcgatgctcg acgaagcgcg cgcagtgggt
     5701 gcagagttca accgtatcgc cggggaaaac tgcctctact tcgaaaccgg acaaggctct
     5761 gcgctatccg ctggcgctaa cttcggcgct gaccaggtga cgatggaagc acgtaactat
     5821 gggctggcgc gtcattacga tccgtttatc gtcaacaccg tggtcggctt tattgggccg
     5881 gagtatctct acaacgaccg ccagattatc cgtgctggct tagaagatca ctttatgggc
     5941 aagctgagcg gcatctctat gggctgtgac tgctgttaca ccaaccacgc tgacgctgac
     6001 cagaacctca acgaaaacct gatgatcctg ctcgccaccg caggctgcaa ctacatcatg
     6061 gggatgccgc tgggtgatga catcatgctc aactaccaga ccaccgcatt ccacgatacc
     6121 gccactgtgc gtcagttact caacctgcgt ccgtcaccgg agtttgaacg ctggctggaa
     6181 agcatgggca ttatggcaaa cggtcgcctg accaaacggg cgggcgatcc gtcactgttc
     6241 ttctgatgac gcggagataa cacatcatgg atcaaaaaca gattgaagaa attgtacgca
     6301 gcgtgatggc gtcaatggga caaacggccc ccgcgccgtc agaagcaaag tgcgccacca
     6361 ccaactgtgc ggcaccggtg acctcggaaa gctgcgcgct ggatttaggt tctgcggaag
     6421 caaaagcgtg gattggtgtt gaaaacccgc atcgcgcaga cgtattaaca gaactgcgtc
     6481 gcagcaccgt ggcccgcgtt tgtaccggtc gtgccggtcc gcgtccgcgt acgcaggcgt
     6541 tgctgcgttt cctggccgat cactctcgtt cgaaagatac cgtactgaaa gaagtgccgg
     6601 aagagtgggt gaaagcgcag ggcttgctgg aagtacgctc cgaaattagc gacaaaaatc
     6661 tctacctgac tcgcccggat atgggccgcc gcctgtgtgc agaagctgtt gaagcgctga
     6721 aagcacagtg tgttgctaat ccggacgtac aggtcgttat ttctgatggc ctgtcaactg
     6781 atgccatcac cgtgaactac gaagagatcc tgccgccgct gatggcgggc ctgaaacagg
     6841 ccgggctgaa agtcggcacg ccattctttg ttcgttatgg tcgcgtgaag attgaagatc
     6901 agatcggtga gatcctcggc gcgaaagtgg tgatcctgct ggtgggggaa cgtccggggt
     6961 tagggcagtc agaaagcctc tcctgctacg ccgtttactc gccgcgtatg gcgaccaccg
     7021 ttgaggccga tcgcacctgt atctctaaca ttcaccaggg cggcacgccg ccggttgaag
     7081 ccgcagccgt cattgtggat ttggcgaaac gtatgctgga gcagaaagca tccggcatca
     7141 acatgacccg ataaggaggc atcatgccag ctttagattt gattcgaccg tcggtaaccg
     7201 ccatgcgggt gattgcctcc gttaacgctg aatttgcgcg tgaactaaaa ttgccgccgc
     7261 atattcgtag cctcgggctg atttctgccg attctgacga cgtcacctac attgcggctg
     7321 acgaagcgac caaacaagcg atggtcgaag tggtatatgg tcgctcgctg tatgcgggag
     7381 cggcacacgg cccgtcaccg actgccggtg aagtgctgat tatgcttggt gggccaaacc
     7441 cggcggaagt gcgtgccggt ctggatgcga tggttgcaca tattgaaaat ggcgcagctt
     7501 tccagtgggc caatgacgca gaaaacaccg ccttcctggc acatgtagtt tcgcgtaccg
     7561 gttcttatct ctcatcaacc gccgggatca cgcttggcga tccgatggcg tatctggtgg
     7621 caccgccgct ggaagcgacc tacggcattg atgcagcgtt gaaatctgcc gacgtacagc
     7681 tggtgaccta tgtcccgcca ccgtctgaaa ccaactactc ggcagcattt ttaaccggta
     7741 gccaggccgc gtgtaaagca gcctgtaacg cctttaccga tgcagtgctg gaaatcgcgc
     7801 gtaatccaat ccagcgtgcg taacggaggt tgccgatgat caatgcactg ggattgctgg
     7861 aagtggacgg catggtcgcc gcgatagatg cagcggatgc catgctcaaa gcagctaacg
     7921 ttcgtctgct cagtcacgaa gtgcttgacc ctggtcgctt aacgctggtg gtggaaggcg
     7981 atctggcggc gtgtcgtgcg gcgctggacg caggttgtgc tgccgcgatg cgtaccgggc
     8041 gtgtcatcag ccgcaaggag atcggtcggc cagacgatga cacccagtgg ctggttactg
     8101 gctttaaccg ccagccgaag caacccgtaa gggaacccga cgcgccagtt atcgtcgcgg
     8161 aatctgctga cgagttgttg gcgctgttaa catcagtacg tcagggaatg acggcaggag
     8221 aagtggctgc ccactttggc tggccgctgg aaaaagccag aaatgcgctc gaacagctct
     8281 tttctgccgg gacgttacgt aaacgcagta gtcgttatcg tctcaagccc cattaacctg
     8341 tcggaggtgc cgggtgtcct acaacacccg gcattaacat catgaaaaag acccgtacag
     8401 ccaatttgca ccatctttat catgaaccct tacccgaaaa cctgaagctc acgccgaagg
     8461 tcgaagtgga taatgttcat caacgacaga caacggatgt ctatgaacat gctttaacga
     8521 ttaccgcctg gcagcagatt tacgatcagc tgcatccggg caagtttcat tgtgaattta
     8581 cggaaattct actcgatgat attcaggttt ttcgtgaata caccggtctg gcgctgcgtc
     8641 agtcgtgcct ggtctggccg aactcgttct ggtttggcat tccggcgacg cgcggtgagc
     8701 agggatttat cggttcgcaa tgtctgggaa gcgcggaaat cgccacccgc cctggtggca
     8761 ctgaatttga actgagcacg ccggatgatt acacgatcct gggcgtggtg ctttctgaag
     8821 atgtcatcac ccggcaggct aactttttgc ataacccgga tcgggtatta catatgctgc
     8881 gtagccagtc ggcgctggaa gtgaaagagc agcataaagc cgcgctgtgg ggctttgtcc
     8941 aacaggcgct ggcgacgttt tgcgagaacc cggaaaatct ccatcagcct gcagtgcgaa
     9001 aagtgctggg ggataatttg ctaatggcga tgggggcgat gctggaagac gcgcaaccaa
     9061 tggtgacggc ggaaagcatc agtcatcaga gttaccgtag attactttcc cgcgcccgtg
     9121 aatatgtgct ggaaaatatg tctgagccgg tgacggtgct ggacttgtgt aatcaactgc
     9181 atgtcagtcg ccgcacgcta caaaacgcgt ttcacgctat tttaggcatt ggcccaaacg
     9241 cgtggctgaa acgcattcgc ctgaacgccg tacgccgcga actgataagc ccgtggtcgc
     9301 aaagcacaac ggtaaaagac gccgccatgc agtggggatt ctggcatctg gggcaatttg
     9361 ccacggatta ccagcagctg tttgccgaga agccgtcgtt gacgttgcat cagcggatgc
     9421 gggaatgggg gtgagggagt tacacccaat ccctgacctt aataaactca cttaacgccg
     9481 cttctgggct gccatctttt ggctgataat catactccca gcgtaccagt ggcggcattg
     9541 acatcaggat cgactcggtg cgcccgccag tttgcaggcc aaacagagtg ccgcgatccc
     9601 agaccagatt gaactcgaca taacgaccgc gacggtagag ctgaaaattg cactcgcgct
     9661 cgccgtaggc catcgcttta cgtcgctcta caattggtaa ataagcgtcg gtgtagcctt
     9721 tgcctaccgc ctgcataaag gcaaaacagt ggtcgaaatc tggcgtgttc agatcatcaa
     9781 agaacagccc gccaataccg cgctgttcgt tgcgatgttt gaggtagaag taatcgtcgc
     9841 accacttttt gtaacgggga taaacgtctt caccaaatgg caggcacagg tcacgggcgg
     9901 tgcggtgcca gtgaatggcg tcttcttcaa aaccatagaa aggggttaaa tcgaagccgc
     9961 cgccaaacca ccagacgggc tctgcacccg gtttttcggc aataaaaaaa cgcacattcg
    10021 cgtggctggt gggaacatac gggttatgcg gatgcactac cagtgaaacg cccatcgcct
    10081 cgaaactgcg cccggcaagt tccgggcgat gagcggtggc ggaagctggc atcgcctcgc
    10141 cgtggacgtg agaaaagttg acgcctgcct gttcgaaaac accaccatta cgcaacaccc
    10201 gactacgccc gccgccgcca gcttcgcgct gccaactatc ttcgacaaat tctgcgccat
    10261 cgacggcggt cagctgctga caaatcgtat cctgaaggtt gagcagaaac tgtttaacct
    10321 ggtgtgcgtc gggtttcata acttatcgct ttttcgaatg tgctttctgg ttgtcgaacc
    10381 agtggaaata actgatcacg ccttcagcaa tcgctgtggc gattttctga cgaaacgccg
    10441 ccgtgcctaa cagccgctct tcttccgggt tggtgataaa cgaggtttcc accagcaccg
    10501 aaggaaccga cggcgatttc aacaccacaa acgcggcttg ttcggtgttg cggctgtgca
    10561 gtttatgcac cggcttaatc tttttcagaa tatgcgagcc gagcgtcagg ctgtttttaa
    10621 tagtatcggt ttgcaccaga tcaaacagca cttgctgcaa taggtgatcc ttgtcagtcg
    10681 cctttttacc ggcaacttca tcggcgcggt tttcgcgttc agacaggtat ttcgccattg
    10741 cgctacttgc cccacggtta gagagggcaa ataccgaagc accggcagct ttcgggttgg
    10801 taaagccatc ggcatgaatt gacataaaca gatctgcgcc atgtttatgg gcgatttcaa
    10861 cgcgatcgta aagtgggata aacgtatcgc cagaacgcgt taaacgcgca tcaatcccat
    10921 gattacgcaa aatggaacgg acgtttttag caatcgccag caccacatgt ttttctttcg
    10981 aaccgttgcg tccgatcgct ccggtatcaa ttccgccgtg gcctggatcg agaacaacga
    11041 cacgtttgcc gccagatttt ttggctttcg gcttgctgtg tccgttgctg gtttttaaag
    11101 gttcgtcttt ggcgatggct tgcgacattc ctgacaacgt cagggcagcc aaaccggctt
    11161 tcagcacctg gcggcgcgaa gtgagtgttt ttagtggttt aaaagtgctc atacggcctg
    11221 agttgtaata ataaagttcc agatgttata tcgtatcgcg taatccgtta cgacggtttg
    11281 atgttgagaa ttgttttact tttcatttca atagttgaca actccccatt atgccacttt
    11341 ttcaaccgat tttcgtcaga tattggctaa ttcgaccgtt cacgccataa tcagcctttt
    11401 aacccgcaaa aaggtaacga ataccatgga gatacgcgta tttcgccagg aagatttcga
    11461 agaggtcatc accctttggg agcgttgcga cttgctgcgt ccgtggaacg atccggaaat
    11521 ggacatcgag cgtaagatga accatgacgt cagtttgttt ttggtcgctg aggtaaacgg
    11581 tgaagtggtc ggaacggtga tgggcggtta tgacgggcat cgcgggtctg cttattatct
    11641 tggcgtgcat ccagagtttc gtgggcgtgg gattgccaat gcgttgctta atcggctgga
    11701 gaaaaagctg attgctcgtg gctgcccgaa aattcagatc aatgtgccgg aagataacga
    11761 catggtgctc ggaatgtatg aacgcctggg atatgaacac gccgacgtgc tgagtctggg
    11821 taagcgtttg attgaagatg aagagtactg agttccatcc tgtccattat gatgcgcacg
    11881 gacgcctgcg tttacccttg ctcttctggc ttgtgctatt gcttcaggcg cgaacctggg
    11941 tgctgtttgt cattgccggt gcgtcgcgcg agcagggtac tgcgctgcta aatctgtttt
    12001 atcccgatca cgataatttc tggctggggc tgattccagg cattccggcg gtgctggcgt
    12061 ttttgttgag cggtcggcgg gctacatttc ctcgcacttg gcatgtgctc tattttctgt
    12121 tgctgttggc gcaggtggtt ttactttgct ggcaaccgtg gctgtggctg aacggtgaat
    12181 ccgttagcgg tatcggtctg gcgctggtag tggcggatat tgtggcgtta atctggctgc
    12241 tgaccaatcg acgtttacgc gcttgtttta atgaagaaaa agaataacgg cactttttgg
    12301 tgaatttgca ctccaagcaa cgttattgaa taaccaaagg aagtgacatg aaatcgctgc
    12361 gtttaatgtt atgcgctatg ccgttgatgc tgaccggctg ttccacgatg tcgtcagtta
    12421 actggtcggc cgctaacccg tggaactggt ttgggtcgtc caccaaagtg agcgagcagg
    12481 gcgttggtga attaacggcg tccacaccac tgcaagaaca agccattgcc gatgcgcttg
    12541 atggcgatta tcgcctgcgc agcggaatga aaaccgcgaa cggcaacgtg gtgcgctttt
    12601 ttgaagtgat gaagggcgac aacgtggcga tggtgattaa cggcgatcaa ggcacgatca
    12661 gtcgcattga cgtgctggat agcgatattc ctgctgacac cggtgttaaa atcggtacac
    12721 cgtttagcga cctttacagc aaagcatttg gcaattgcca aaaagctgat ggtgatgata
    12781 atcgtgccgt cgaatgtaaa gccgaaggca gtcaacatat tagctaccag ttcagcgggg
    12841 aatggagtgg tcctgaaggg ttaatgcctt cggacgatac cctgaaaaac tggaaagtca
    12901 gtaaaattat ctggcggcgt taatttgcgt ctgaacaaac cgccgccgcg aaaaaacacg
    12961 taaaatagcg ctcaacaatg ccacggattg cgtggcatta ttattttcag gaggaacaat
    13021 gtctcaggtt cagagtggca ttttgccaga acattgccgc gcggcgattt ggatcgaagc
    13081 caacgtgaaa ggggaagttg acgccctgcg tgcggccagt aaaacatttg ccgacaaact
    13141 ggcaactttt gaagcgaaat tcccggacgc gcatcttggt gcggtggttg cctttggtaa
    13201 caacacctgg cgcgctctga gcggcggcgt tggggcagaa gagctgaaag attttccggg
    13261 ctacggtaaa ggccttgcgc cgaccaccca gttcgatgtg ttgatccaca ttctttctct
    13321 gcgtcacgac gtaaacttct ctgtcgccca ggcggcgatg gaagcctttg gtgactgcat
    13381 tgaagtgaaa gaagagatcc acggcttccg ttgggttgaa gagcgtgacc tgagcggctt
    13441 tgttgacggt acggaaaacc cggcgggtga agagacgcgt cgcgaagtgg cggttatcaa
    13501 agacggcgtg gatgcgggcg gcagctatgt gtttgtccag cgttgggaac acaacctgaa
    13561 gcagctcaac cggatgagcg ttcacgatca ggagatgatg atcgggcgca ccaaagaggc
    13621 caacgaagag atcgacggcg acgaacgtcc ggaaacctct cacctcaccc gcgttgatct
    13681 gaaagaagat ggcaaagggc tgaagattgt tcgccagagc ctgccgtacg gcactgccag
    13741 tggcactcac ggtctgtact tctgcgccta ctgcgcgcgt ctgcataaca ttgagcagca
    13801 actgctgagc atgtttggcg ataccgatgg taagcgtgat gcgatgttgc gtttcaccaa
    13861 accggtaacc ggcggctatt atttcgcacc gtcgctggac aagttgatgg cgctgtaagc
    13921 gtttctgatt cattgattaa aggccaggca gtaacattac tgactggcct ttttattact
    13981 tttgctttaa cgccgcatac acctgatcaa ctacccaacc atacgttgca atcggcagct
    14041 gaccgctttc gaacatattg ggattctttt gcggatcggc aaccggcgaa tgtggcttgt
    14101 tgcttaacat cacaatcgcc atatgattca ccggatcgat aacggtcacc gttccggtcc
    14161 agccagtgtg accgtaggtt tgcgggcttg ccagcgtgcc aaacgtcggc gtcatggtgg
    14221 cattaccatt cacgcgccag ccgaggccaa aagtggcatc ttccttagag ctggtggtga
    14281 acatcttcac cgtttccgca ctgaacagct gcacatcgcc atagcccccg ccgttcagca
    14341 tcgtttgcat taacaccgca atatcgccgg tattggaaaa caaacctgcg tgcccggaaa
    14401 cgccgcccat cgaataaaag gctttttcat cgtgcacctg accccagaga gtggaggtgc
    14461 ggatattcgg gaaatggatc acgccatcgc gggtattgcc gttcaattcc gtggcggcaa
    14521 tttgctgcgg tttaaagcct ttcagcagcg ggttaaatac cgtatgtgtc aggccgagcg
    14581 ggcgataaat cgactcttca acatagcggt caagcggctg accggtgacc gactcgacga
    14641 taaatccaag cagcatataa tcgacatcac tgtaaatatg cttgctgccg ggctgatact
    14701 ccagcggcgt gcgcttgatc atctccagcg tctggccttt atcctgggaa tataacgcgc
    14761 ccgcgaccgc tttattcgga tattgtgggt ccgccgggaa accgccgctg tgatgcagca
    14821 ggtcagaaat ccgcagcgtg tttttgcctt tgatggtgtc atttggacta tcggcaaacc
    14881 ccggaatata tttcgcaatc cgatcgtcag gatgcagttt gccttcgctc atcagctttt
    14941 gcagggcgaa gttcgtggcg tacattttgg tgtttgaggc caggtcatac agcgtcccgg
    15001 tggtggcttt gacaggctgt tccatcaaca cgctgccatc gtactttttc gccgctcccc
    15061 aggctttgcg atacacaatc tgattatctt taatgatcag caggtttacg ccgggataac
    15121 cggcgtccac ttgctggcta atccagcgat ccatctggtt aagccgttcg acgttgaacc
    15181 ctgctttctc tggcgaactt tctgtcagaa cagggtattt tgcggcgctg acgctacaac
    15241 taacagccag cagagaaaga tagagcattg tccgtttcat gatatcagtc cagattgacg
    15301 ttacggcagc caaagagcgt ggtgaaaata aacccgcaaa cccatgccac cagaatcccg
    15361 ccagcataaa ccgccattgc cggaaggatg ccttgtgcgg aagtcatcag cggcagcgcc
    15421 accagaccag acggcccaaa ggcgctgttt aagcccatcg gcagacccca ccaggctatc
    15481 aggccgataa acaaaccgcc cgctgcgccg cctaaacagg cggtaataaa cggtttcatg
    15541 cggggcaggg tgacgccgta aatcaacggt tcaccaacgc ccagcagacc gggaataatc
    15601 gccccgcgta cctgactgcg taatgcactg tgcggttgcg cccgccagta gagtgccagc
    15661 gccgcgccca cctggcccgc gcctgccatt gaaaggatgg gaaataagct gttgaatccc
    15721 tggctgtcca ttaacgcgag gtaaacagga ataaagccct gatgcacgcc aaataccacg
    15781 gcgatcagga acagcccggc taaaaccgca caaccgaacg gattactgtt caggtgcata
    15841 aacagccatg acataccttc gaataaccag ccacccagcg gcataatgat caggtacgca
    15901 agcgtagcgg tgatcagcag ggtgattaac gaggtcagca gcatgtcgag atcgtccggc
    15961 ataaagcggc gcaccatgcc ttcaatgcgg gcgcaggccc acgcggcaat cagcacgccg
    16021 ataatattgc cgcgcggatc gatgggcaga ccaaagaaat cgtgaaaacc ggcgtagtaa
    16081 ccggtggttg cagcggggtt ataaccgagc aaaaagagtg cggcaataat tgcgccattg
    16141 acgcccgtgc cgccaaatgc ctgggcggcg ttatagccca ccagaatcac caggaaagtg
    16201 aacaaacctt tgctgaacac cttcataaaa ttcagcgcgt cgggtagcgt gccctgagca
    16261 tctgccggaa cgtgcatcac cgtggcaatt aacgtcgcta tccccagcag cagaccggcg
    16321 gcaataaaac cggggatcag cggcgtgaag atggtggcga atttggcgag aaattgttgc
    16381 acgccggagg tttgtttggc ttttaactga cgtttgttct gggcggcgat ttctgcggca
    16441 tcctgtaccg gtgcctcgcc cagcagctcg ctcatcgctt ttgcggcacg atgcgcttta
    16501 ccaggcccaa aaacgacctg cacctggtcg ctggtcaaaa tgacgccctt cacgccttcc
    16561 agcgttttga tattgggatc aaccagtgaa ctgtcatgta cacccagacg caggcgcgtc
    16621 atacagttac cacaactggc gatatttccc ggtccgccga cacgggtaag aatggtgttc
    16681 agaagttcac tgctgatctc tttggccatg ggttattcct tgtctaaaac ctgacgaata
    16741 aagccgccgt gttgatccag gcgtttttta gcttctgcgg catcgagatt tttcagcacc
    16801 atcacaatgg ccgttttaca gttgcgctcg caggcaatta acgccgcttc cgcttgttcg
    16861 gcattacatc cggtggcgtt tttaacgata ttgacctggc gcacatgcag tttttcgttg
    16921 gtggcgacca catcgaccat caggttgccg aacactttgc cagatttaat catcagcccg
    16981 gtggaaagca tattgagcac cagtttctgc gctgtgcctg ctttcattcg tgaagaacca
    17041 gtgaccactt cggcacctac aatcggtgta atggcaaact cggcggtggt ggaaacggca
    17101 ctccccggat tacaggaaat tcccactgtg cggcagccga gctggcgtgc gtattccagt
    17161 ccggcaatca catacggcgt gcgaccgctg gcagcaatgc caaccaccac atcctgtgcc
    17221 gttaaattaa tatttttcag atcgttaaca ccaccttccc ggctatcttc agcgccttcc
    17281 accgcgtgct gaatggcata ttcgcctcca gcaatcaaac caaccaccag acccggtttc
    17341 acgccgtagg tgggcggaca ttcgctggca tccagaatcc ccagacgacc ggatgttccc
    17401 gcaccgagat aaatcagacg accgccgccg ctgacctggg cgtggataac atcgatcgcc
    17461 gcggcgatat ccggcaggac gcgctcaacg gcaagcggta cggttttatc ttcatcgttg
    17521 ataatccggc acatttccag cgtcgatacg cggtcaattt cagccgaggc ggtgttcgag
    17581 ccttcagtaa tcatcttttc tagttgcatc ggtggtgacc ttactattta cgatcaaata
    17641 attgcagatg tatggaatca tatatcccga attgacagac ttaaaggaat cattgagtct
    17701 acactcgcag agtgtgaaga gcgtcataag aacttaagcg gaatttatgc tgccagccca
    17761 ggagagtgga agatgttgta cctgacgaaa ataagcaatg cggggagtga attcaccgaa
    17821 aacgagcaaa aaatcgctga ctttctgcag gccaacgtga gtgagctgca atccgtctct
    17881 tcgcgcaaga tggcgaaaca gctcggcatc agccagtcga gtattgtgaa gtttgcccag
    17941 aagctcggcg cacaaggatt tactgaattg cgtatggcgt taattggcga atacagtgcc
    18001 agccgggaga aaacgaatgc cacggcgctg cacctgcaca gttcgattac cagtgatgat
    18061 tcgctggaag tgattgcccg caaactgaat cgcgaaaaag agctggcgct ggagcaaacc
    18121 tgcgcgttgt tcgactacgc gcggctgcaa aaaatcattg atgtcatcag taaagcgcaa
    18181 tttattcaga taacgggcct gggcggatcg gcgctggtgg ggcgcgatct gtcgttcaaa
    18241 ctgatgaaaa ttggatatcg cgtggcctgc gaagccgaca cgcacgtaca agccaccgtt
    18301 tcccaggcgt tgaaaaaagg cgatgtacaa atcgctatct cttacagcgg cagtaaaaaa
    18361 gagattgtct tgtgcgcaga agcggcacga aagcaggggg caacggtgat tgccattact
    18421 tctctggctg actctccgct gcggcggctg gcgcatttca cgctcgatac cgtttccggt
    18481 gaaaccgaat ggcgtagctc gtcgatgtct acccgcacgg cgcaaaattc tgttaccgat
    18541 ttactgtttg ttggtcttgt gcaactcaat gatgtcgagt cattgaaaat gattcagcgc
    18601 agcagtgagc taacacaacg cctgaaatga tgcataaagc agcgactgga ttgagatttt
    18661 cctgaattag tgagctgatc cgcagcaata ttttgtttat cctgtatttt cagagggaat
    18721 ggagtgtaac gctctgtatt aacaaggaga gcattaaaat gggtaaactc acgggcaaga
    18781 cagcactgat tacgggcgca ttgcagggaa ttggcgaagg aattgccaga acttttgcgc
    18841 gtcatggcgc aaacctaatc ttgctggata tctcccctga gatcgaaaag ctggcggacg
    18901 aactgtgtgg tcgtggtcat cgctgtacgg cggttgtcgc cgatgtgcgt gacccggcgt
    18961 cggtagccgc agctatcaaa cgcgcgaagg aaaaagaagg gcgcattgat atcctggtga
    19021 ataacgcagg cgtttgtcgt ctgggcagtt tcctcgatat gagcgatgaa gatcgcgatt
    19081 tccatattga catcaatatt aaaggcgtat ggaacgtcac gaaggcggtg ctgccagaga
    19141 tgattgcgcg caaagatggt cgcattgtga tgatgtcttc agtcactggt gatatggtgg
    19201 ccgatccggg tgaaacggcg tacgccttaa cgaaagcggc gattgttggc ctgacaaaat
    19261 cgctggcggt ggagtacgcg caatccggta ttcgcgttaa cgccatctgc ccggggtacg
    19321 tccgcacgcc aatggcggaa agcattgccc gccagtcgaa cccggaagat ccagagtcgg
    19381 tgctgactga tatggcaaaa gcaatcccgc tgcgtcgcct cgccgatccg ctggaagtcg
    19441 gcgaactggc ggccttcctc gcatcggatg aatccagcta tttaaccggt acacagaatg
    19501 tgattgatgg cggcagcaca ctgccggaga cggttagcgt cggtatctga ttcacctctg
    19561 tttcctccct gcatgtgtgg ggaggatttc gttttaaacc cggttcttat tcccttttca
    19621 acttccaaat caccaaacgg tatataaaac cgttactcct ttcacgtccg ttataaatat
    19681 gatggctatt agaaagtcat taaatatata agggtgcgca atggccgtta acttactgaa
    19741 aaagaactca ctcgcgctgg tcgcttctct gctgctggca ggccatgtac aggcaacgga
    19801 actgctgaac agttcttatg acgtctcccg cgagctgttt gccgccctga acccgccgtt
    19861 tgaacagcaa tgggccaaag ataacggcgg cgacaaactg acgattaaac aatctcatgc
    19921 cgggtcatca aaacaggcgc tggcgatttt acagggctta aaagccgacg ttgtcactta
    19981 taaccaggtg actgacgtac aaatcctgca tgacaaaggc aagctgatcc cggcggactg
    20041 gcagtcgcgc ctgccgaata atagctcgcc gttctactcc accatgggct tcctggtgcg
    20101 taagggcaac ccgaagaata tccacgactg gaacgacctg gtgcgctccg acgtgaagct
    20161 gattttcccg aacccgaaaa cgtcgggtaa cgcgcgttat acctatctgg cggcatgggg
    20221 cgcagcggat aaagctgacg gtggcgataa agccaaaacc gaacagttta tgacccagtt
    20281 cctgaaaaat gttgaagtgt tcgataccgg cggtcgtggc gcaaccacca cttttgccga
    20341 gcgtggcctg ggcgatgtgc tgattagctt tgaatcggaa gtgaacaaca tccgtaaaca
    20401 gtatgaagca cagggctttg aagtggtgat cccgaaaacc aatattctgg cggaattccc
    20461 ggtggcgtgg gtcgataaaa acgtgcaggc caacggtacg gaaaaagcag caaaagccta
    20521 cctgaactgg ctctacagcc cgcaggcgca gaccatcatc accgactatt actaccgcgt
    20581 aaataacccg gaagtcatgg acaaactgaa agataaattc ccgcagaccg agctgttccg
    20641 cgtggaagac aaatttggct cctggccgga agtgatgaaa acccacttca ccagcggcgg
    20701 cgagttagac aagctgttag cggcggggcg taactgatgt ttgcagtttc atcaagacgc
    20761 gtgctgccgg gctttacctt aagcctcggc accagtctgc tgtttgtgtg cctgattttg
    20821 ctgctgccgc tctccgcgct ggtgatgcag ctggctcaga tgagctgggc gcagtactgg
    20881 gaggtgatca ccaacccaca agtggtcgcg gcctacaaag tgacgctgct gtcggcgttt
    20941 gtggcatcga tttttaacgg cgttttcggt ctgctgatgg cgtggatttt aacacgttat
    21001 cgctttccgg gccgcacgct gcttgatgcg ctgatggatt taccctttgc gctgccaacg
    21061 gcagtcgccg gtttaacgct ggcctcgctc ttttccgtaa acggttttta cggtgaatgg
    21121 ctggcgaagt ttgatatcaa agtcacctat acctggcttg gtattgcggt ggcgatggcc
    21181 tttaccagca ttccgtttgt ggtgcgtacc gtgcagccgg tgctggaaga gttaggcccg
    21241 gaatatgaag aagcggcgga aacgcttggt gcaacgcgct ggcagagttt ctgcaaagtg
    21301 gtgctgccgg aactttctcc agcgctggtg gcgggcgtgg cgttgtcgtt tacccgtagt
    21361 cttggtgagt ttggcgcggt gatatttatc gccgggaaca tcgcctggaa gacggaagtg
    21421 acgtcgctga tgatttttgt gcgcttgcag gagtttgatt acccggcggc gagcgcgatt
    21481 gcttcggtga tcctcgcggc atccctgctg ctgctgttct caattaacac tctgcaaagt
    21541 cgctttggtc ggcgtgtggt aggtcattaa tggcggaagt tacccaattg aagcgttatg
    21601 acgcgcgccc gattaactgg ggcaaatggt ttctaattgg catcgggatg ctggtttcag
    21661 cgttcatcct gctggtgccg atgatttaca tcttcgtgca ggcattcagc aaggggctga
    21721 tgccggtttt acagaatctg gccgatccgg acatgctgca cgccatctgg ctgacggtga
    21781 tgatcgcgct gattgccgta ccggtaaacc tggtgttcgg cattctgctg gcctggctgg
    21841 tgacgcgctt taacttccct ggacgccagt tactgctgac gctactggac attccgtttg
    21901 ccgtatcgcc ggtggttgcc ggtctggtgt atctgctgtt ctacggctct aacggcccgc
    21961 tgggcggctg gctggatgaa cataatctgc aaattatgtt ctcctggccc ggaatggtgc
    22021 tggtcaccat ctttgtgacg tgtccgtttg tggtgcgcga gctggtgccg gtgatgttaa
    22081 gccagggcag ccaggaagac gaagcggcga ttttgcttgg cgcgtctggc tggcagatgt
    22141 ttcgccgcgt cacattgccg aacattcgct gggcgctgct ttatggcgtg gtgttgacca
    22201 acgcccgcgc aattggcgag tttggcgcgg tgtcggtggt ttccggctcg attcgcggcg
    22261 aaaccctgtc gctgccgtta cagattgaat tgctggagca ggactacaac accgtcggct
    22321 cctttaccgc tgcggcactg ttaacgctga tggcgattat caccctgttt ttaaaaagta
    22381 tgttgcagtg gcgcctggag aatcaggata aacgcgcaca gcaggaggaa catcatgagc
    22441 attgagattg ccaatattaa gaagtcgttt ggtcgcaccc aggtgctgaa cgatatctca
    22501 ctggatattc cttcaggtca gatggtcgcg ttgctggggc cgtccggttc cgggaaaacc
    22561 acgctgctgc gcattatcgc cgggctggag catcaaacca gcgggcatat tcgcttccac
    22621 ggcaccgacg tcagccgcct gcacgcgcgc gatcgcaagg tcggtttcgt gttccagcat
    22681 tacgcgctgt tccgtcatat gacggtgttc gacaatatcg cttttggcct gacggtgctg
    22741 ccacgtcgcg agcgcccgaa tgccgcagcc atcaaagcga aagtgacaaa attgctggag
    22801 atggtgcagc ttgctcatct ggcagatcgt tatccggcac agctttccgg cggccagaaa
    22861 cagcgcgtgg cgctggcgcg cgcgctggct gtagaaccgc aaattctgct gcttgatgaa
    22921 ccgtttggcg cgctggatgc gcaggtgcgt aaagagctgc gtcgctggct gcgtcaactg
    22981 catgaagagc tgaaattcac cagcgtgttc gtgacccacg accaggaaga ggcgaccgaa
    23041 gtagccgatc gtgtagtggt gatgagtcag gggaatattg aacaggctga tgcgccgaat
    23101 caggtatggc gcgaaccagc gacccgtttt gtactcgaat ttatgggcga agtgaaccgc
    23161 ctgcagggaa ccattcgcgg cgggcagttc catgttggcg cgcatcgctg gccgctgggc
    23221 tacacacctg cgtatcaggg tccggtggat ctcttcctgc gcccgtggga agtggatatc
    23281 agccgccgta ccagtctcga ttcgccgctg ccggtacagg tactggaagc cagcccgaaa
    23341 ggtcactaca cccaattagt ggtgcagccg ctggggtggt acaacgaacc gctgacggtc
    23401 gtgatgcatg gcgacgatgc cccgcagcgt ggcgagcgtt tattcgttgg tctgcaacat
    23461 gcgcggctgt ataacggcga cgagcgtatc gaaacccgag atgaggaact tgctctcgca
    23521 caaagcgcct gataggttga gtgaatgtta aacgcccgga ggcgcttccc gcgatccggg
    23581 ctttttaatg gcaaggtttg taacctgtag acctgataag acgcgcaagc gtcgcatcag
    23641 gcaacaccac gtatggatag agatcgtgag tacattagaa caaacaatag gcaatacgcc
    23701 tctggtgaag ttgcagcgaa tggggccgga taacggcagt gaagtgtggt taaaactgga
    23761 aggcaataac ccggcaggtt cggtgaaaga tcgtgcggca ctttcgatga tcgtcgaggc
    23821 ggaaaagcgc ggggaaatta aaccgggtga tgtcttaatc gaagccacca gtggtaacac
    23881 cggcattgcg ctggcaatga ttgccgcgct gaaaggctat cgcatgaaat tgctgatgcc
    23941 cgacaacatg agccaggaac gccgtgcggc gatgcgtgct tatggtgcgg aactgattct
    24001 tgtcaccaaa gagcagggca tggaaggtgc gcgcgatctg gcgctggaga tggcgaatcg
    24061 tggcgaagga aagctgctcg atcagtttaa taatcccgat aacccttacg cccattacac
    24121 cactaccggg ccggaaatct ggcagcaaac cggcgggcgc attacccatt ttgtctccag
    24181 catgggaaca accggcacta tcaccggcgt ttcacgcttt atgcgcgaac aatccaaacc
    24241 agtaacgatt gttggcctgc agccggaaga aggcagcagt attcccggca ttcgccgctg
    24301 gcctgcggaa tatctgccgg ggattttcaa cgcttctctg gtggatgagg tgctggatat
    24361 tcatcagcgc gatgcagaaa acaccatgcg cgaactggca gtgcgggaag gaatattctg
    24421 tggcgtcagc tccggcggcg cggttgccgg agcactgcgg gtggcaaaag ctaaccctgg
    24481 tgcggtggtg gtagcgatca tctgcgatcg tggcgatcgc tacctttcca ccggggtgtt
    24541 tggggaagag cattttagcc agggggcggg gatttaagat tattgcgcag gcaccgtttt
    24601 atccgtctgc gcaattagcg catgtaaaaa aggctgtgcg ttctcatcgc tcttaccggg
    24661 gatcttcaca atatacggct tcccggtaat cgatgacgac gaagcaacct tatcaataaa
    24721 ctgctcggca gtgtcaatgc ggttacgggt attgcccagc ttcagacgca gatgagaaac
    24781 cgcttcatcg caggtatgtt catcaccgtt gcgcacaaat atcaaatcct ttttctgtgc
    24841 taatccctcc agcatggcgt tgatgcgggc ttcttcatgg gcagttaact tcgcgtaagc
    24901 ggggagtgtc attagtagtg taatgaccag acaaatgatt ttcttcattg aattacctta
    24961 tccttgtttt gtgatagccg atttgactgc cagaatcagg taaagtttcg gttggaatac
    25021 gatgaggatc atcggcaagg agaaaaaatt aaatgagtag tttgttgttg tttaacgata
    25081 agagcagggc gctgcaggcg gatatcgtcg ccgtgcagtc gcaggtggtt tacggcagcg
    25141 tgggcaacag cattgccgtg cctgctatca aacagaacgg tctgaatgtt tttgccgtgc
    25201 cgacggtatt gctgagcaat acgccgcatt atgacacttt ctacggtggt gcgattccgg
    25261 atgaatggtt tagcggttat ttgcgtgcgc ttcaggagcg tgatgcgctg cgccagctgc
    25321 gcgctgtaac cacgggctat atgggaacgg catcgcaaat caaaatcctt gccgagtggc
    25381 tgactgcgct acgcaaagac catcctgacc tattgatcat ggtcgatccg gtgattggcg
    25441 atattgatag cggaatttat gtcaaacctg accttcccga ggcgtatcga caatatttac
    25501 tgccgctggc gcagggaatt acccccaata tctttgagct ggaaatcctg accggtaaaa
    25561 attgccgcga tctcgacagt gccattgctg ccgcaaaaag tctgctttca gacacattaa
    25621 aatgggtggt gattaccagc gcctccggta atgaagaaaa tcaggagatg caggtggtgg
    25681 tggtcagtgc cgacagcgtg aatgtcattt cccattcacg ggtaaaaacc gacctgaaag
    25741 ggactggcga cctgttttgt gctcagctta tcagtggttt gctgaaaggg aaggcgttaa
    25801 acgatgcggt gcaccgtgcg gggttgcgcg tactggaagt gatgcgctac acccagcagc
    25861 atgagagcga tgaattgatt ttgccgccgc tggcggaagc ataaaaaatg gcgccgatgg
    25921 gcgccatttt tcactgcggc aagaattact tcttgatgcg gataaccggg gtttcaccca
    25981 cggttacgct accggacagt ttgatcagtt ctttgatttc gtccatgttg gagataacaa
    26041 ccggagtcag ggtagacttg gctttctctt ccagtagcgg cagatcaaat tcaatgacag
    26101 tatcgccaac tttcacgcgc tgaccttctt cagcaatacg cttgaagcct tcgcctttca
    26161 gttcaacggt gtcgataccg aagtggacga acagttcaac gccgctatca gattcgatag
    26221 agaacgcgtg attggtttca aagattttac caatggtacc gtctacaggc gcgaccattt
    26281 tgttacccgt tggtttgata gcaataccat caccaacgat tttttccgca aaaacgacat
    26341 ccggcacgtc ttcgatattg acgatctcgc cagagagcgg agcaatgatc tcaatagttc
    26401 cggtatcctt cttgtcgtcg gaaaccagag atttcagttt atcgaacaaa cccatgatct
    26461 tctcctaagc agtaaattgg gccgcatctc gtggattagc agattgtttt ttcttcaatg
    26521 aacttgttaa ccagcgtcat taactcgtcc gttgtcggtt gagcaagagc ctgctctgct
    26581 aacaccttcg catcttcgaa gttcgtgtta cggataatct tcttaatgcg cgggatagaa
    26641 atggcgctca tagagaattc gtccagaccc atccccagca acagaagtgt agcacgttca
    26701 tcgccagcaa gctcaccaca catgccagtc catttgcctt cagcatgaga agcatcaata
    26761 acttgcttga tcaagttcag cacggacggt gacattggtt ggtaaaggtg tgaaatcata
    26821 tcattaccac ggtcaactgc cagagtgtac tgcgttaaat cattggtgcc gatactaaag
    26881 aaatcaactt ctttggctaa atgacgtgca attgttgcgg cagccggtgt ttccaccatt
    26941 acgccgattt caattgactc gtcaaacgct ttaccttcgt cgcgcagttc ctgtttgtag
    27001 atttcgatct ctttgcgcag tgcacgcact tcttcaacag agatgatcat cgggaacata
    27061 atgcgcaatt taccgaaagc cgaggcacgc aggatagcgc ggagctgatc gcgcaggatc
    27121 tctttacgat ccatcgcgat acggatagcg cgccagccga ggaacgggtt ctcttctttc
    27181 gggaagttca tgtatggcag ctctttgtcg ccgccgatgt ccatggtacg aacgataacc
    27241 gcctgcgagc cacacgcttc agccactgct ttgtaagcag caaactgttc ttcttcagtg
    27301 ggcagcgcgt cgcggtccat gaacaggaac tcagtacgat acagaccaac gccttcagcg
    27361 ccgttacgct ctgcaccttc aacgtcacga accgtaccaa tgttagcgca cacttctacc
    27421 tggtgaccgt ccagcgtaat agctggcaga tctttcagtt tagcaagctc tgctttttca
    27481 gaagccactt gctcctgaac agcgcgcatt ttatcaataa cttcgttggt tggattgacg
    27541 taaacctgat tatttacggc atccagaatc agatagtcgt catttttcac ctgagaggtg
    27601 acgctaccgg tacccacgat agcaggcagt tccagagaac gcgccatgat agaggtgtgg
    27661 gaagtacggc cacccgcgtc ggtgatgaaa cccagcacct tcttcaggtt cagctgtgcg
    27721 gtttcggacg gcgtcaggtc agcggcaacc agaatgactt catcctgaat ggcgctcagg
    27781 tcgataatct tcaggcccag gatgttgcgc agcaggcgct taccgatatc acgtacgtca
    27841 gccgcacgtt ctttcaggta ttcatcatcc agctcttcca gggcagaagc ctgaccttcg
    27901 ataacttcat gagcagctgc gtcagctgtc atgtgcttat ctttaatcag ggctatgatt
    27961 tcctgctcca gctcctcatc ttcgagcagc ataatatgcc cttcaaagat ggcttctttt
    28021 tcttcaccga acgtttcacc agctttcgtt ttgatcgttt ccagctgggc tgatgccttg
    28081 gcacgaccgc tcagaaaacg ttcaacttcc tgatcaacct ggtcggcaga aatttttttc
    28141 cggtcaatga caatttcgtc ttctttcaga agcagagctt taccgaaagc gatacccggg
    28201 gatgctaaaa tgcctgaaat cataacccta ccttacttgt gactgatttt taaaagaacc
    28261 cgggaaatta ctcgagttcc gccatcagtt taaccagatg ttcaaccgct ttctgctcgt
    28321 cttcgccttc tgcggagata gtcacaacgg taccttgagt caggcccaga gtctgcagtt
    28381 taaacaggct tttcgcgctg gcgcttttgc cgttggaagt cacagtaatt tcagaagtga
    28441 agcccttagc ttcttttaca aactgggcag cagggcgggt gtgcagaccg ttcggagcgg
    28501 taatggtaac ttcttgctgg aacattgtat ttccccaact tataggttta gtgttgtgga
    28561 actaaagtct agcctggcgg ttcgacttta gcctgtattg ttagcgccag tttttaacag
    28621 acgcgacgca cgaagagcgc aatacagtcc attgctggcg ggtgttgacc gctgacgttt
    28681 cgtacgtcat taaacattat gccgctaaag gaagaattga accaaatcat aaaatcgatt
    28741 cagctttggc ttttcctgta acgattaatt tcgcgcatca aaataattag tctggttaaa
    28801 taccagttcc gacaggctga atcaatgcca ggaggggcga aagtttgaag caggccacaa
    28861 aaaagcacct aaaaaggtgc ttttttacgc atttttaaca agctggcatt actgttgcag
    28921 ttctttctca gtgaagagat cggcaaacaa tgcggtgctt aaataacgct cacccgatga
    28981 cggtagaata accacaatat tcttgttggt aaagctttca tcttcttgta gtttcaacgc
    29041 cgcagcaaca gctgctccag aagagatacc tgcaagaata ccttcttctt ccatcagacg
    29101 acgcgcggta gaaatcgctt cttcattggt gatgccaatg actttatcga ccagcttgag
    29161 atcgaggtta gctgggataa aaccagcgcc aataccctga attttatgcg ggccaggttt
    29221 aatctcttca ccagccagtg cctgggcgat aactggagaa tcggttggct caacggcgac
    29281 agagataaga tcggtcttgc ctttggtgcc tttaatgtag cggctgacgc cagtcagcgt
    29341 accgccagtc ccaacgccag caataaatac atcaacctga ccgtcggtat cttcccagat
    29401 ctccggaccg gtggtttttt cgtgaatttc agggtttgcc ggattgctga attgttgcag
    29461 cagcaggtat ttctctggat tgctggcgac aatttcttct gctttttgga ttgcgccttt
    29521 catgccttta gcaccttccg tcagcaccag gtttgcacct aacgctttca gcagcttgcg
    29581 gcgttcaata ctcatggttt ctggcatggt cagggtgagt ttgtaaccgc gagcggcagc
    29641 tacataggcc agtgcaatcc cggtattacc gctggtcggt tcaaccagtt caacgcctgg
    29701 tttcagcacg ccgcgctttt cggcatccca aatcatgttg gcaccgatac ggcacttaac
    29761 gctgaagctg gggttacgag attccacctt cgccagaatg cgtccgttac cgatgcgatt
    29821 caggcgaacc agcggcgtgt gaccgatagt cagcgagtta tcttcaaaaa tcttactcat
    29881 ggcctgtcct taactgtatg aaattgggat acaacaggta gcatacccgc tcagagaata
    29941 tgcggaagta aggatttagc atatctatat acagaaggga aataatgaca tgcaagatgg
    30001 aataaggggc ggcataagcc acccctgttt cacacaaacg gtttacaaaa taaccggtag
    30061 attgttaccg ccacatcgcg tgtttatcgc gatagcaatc gacccacatc gccgtcgcgc
    30121 cacaaacggc aacgggcatg atgaacagat taagcagcgg gatcatcgta aacaggctgg
    30181 ttaaagcacc aaactgcata ttggtgattt tgcgtgtgcg cagggcggtg cgcatctctt
    30241 taaacggcac tttgtggtta tcgaaggggt aatcgcaata ctggatggct aacatccagg
    30301 cgctaaacag gaaccacagt accggcgcga cggtttgccc aataccaggg attaagtaaa
    30361 gaattagcaa tacaattgcg cgcggcagat accaggcaaa tttttgccat tcgcgtttca
    30421 tgattcgcgg cacatctttc atgataccga aaatcccggt atctggcggt gtagcgccag
    30481 tcaatcgtgc ttccagttgt tcagccaata aaccgttaaa cggagcggca atccagttag
    30541 caatcgtgga gaagaaatag ccaaacacta acagcacaga gatgacagcc agaggccaca
    30601 acagataact cagccattgt agccagtccg gaacgtaact catgagagtc gggatccaga
    30661 catcgagctg tgtaaagagc caccagaatg cgccccccat caacaaaata ttgaccagca
    30721 gcggtaaaat aacgaaacgc cgaatcccag gttgcgagac gagcttccag ccttgcgcaa
    30781 aatagtaaaa accgctgcgt ggggcagatg tgaatgatga aaccataatc aggatgagct
    30841 ccttttgacc aatcccagga aaattctgcg tattttaccg ggtaattgcg caatggacag
    30901 ttaggatatg ttcgaaaaaa cagcaaaaag cacgatttca tctatctttg tgctgtgaaa
    30961 gttaatagtg cacttgcact tgaggtaatc ggcaaatact cttagtgagt aaatgtttgc
    31021 cgtggtggca aggtgttaga acaacagaga atataatgat gcaggatttg cgtctgatat
    31081 taatcattgt tggcgcgatc gccataatcg ctttactggt acatggtttc tggaccagcc
    31141 gtaaagaacg atcttctatg ttccgcgatc ggccattaaa acgaatgaag tcaaaacgtg
    31201 acgacgattc ttatgacgag gatgtcgaag atgatgaggg cgttggtgag gttcgtgttc
    31261 accgcgtgaa tcatgccccg gctaacgctc aggagcatga ggctgctcgt ccgtcgccgc
    31321 aacaccagta ccaaccgcct tatgcgtctg cgcagccgcg tcaaccggtc cagcagccgc
    31381 ctgaagcgca ggtaccgccg caacatgctc cgcgtccagc gcagccggtg cagcagcctg
    31441 cctatcagcc gcagcctgaa cagccgttgc agcagccagt ttcgccacag gtcgcgccag
    31501 cgccgcagcc tgtgcattca gcaccgcaac cggcacaaca ggctttccag cctgcagaac
    31561 ccgtagcggc accacagcct gagcctgtag cggaaccggc tccagttatg gataaaccga
    31621 agcgcaaaga agcggtgatt atcatgaacg tcgcggcgca tcacggtagc gagctaaacg
    31681 gtgaactgct tcttaacagc attcaacaag cgggcttcat ttttggcgat atgaatattt
    31741 accatcgtca tcttagcccg gatggcagcg gcccggcgtt attcagcctg gcgaatatgg
    31801 tgaaaccggg aacctttgat cctgaaatga aggatttcac tactccgggt gtcaccatct
    31861 ttatgcaggt accgtcttac ggtgacgagc tgcagaactt caagctgatg ctgcaatctg
    31921 cgcagcatat tgccgatgaa gtgggcggtg tcgtgcttga cgatcagcgc cgtatgatga
    31981 ctccgcagaa attgcgcgag taccaggaca tcatccgcga agtcaaagac gccaacgcct
    32041 gatacactta aggcaaatta actcctcttc gaacccccgc ttgtcggggg tttttagcat
    32101 tgatggtgcg atatggaatc aatcgaacaa caactgacag aactgcgaac gacgcttcgc
    32161 catcatgaat atctttatca tgtgatggat gcgccggaaa ttcccgacgc tgaatacgac
    32221 aggctgatgc gcgaactgcg cgagctggaa accaaacatc cagaactgat tacgcctgat
    32281 tcgcctaccc aacgtgtagg cgctgcgccg ctggcggctt tcagccagat acgccatgaa
    32341 gtaccaatgc tgtcactgga taacgttttt gatgaagaaa gctttcttgc tttcaacaaa
    32401 cgtgtgcagg accgtctgaa aaacaacgag aaagtcacct ggtgctgtga gctgaagctg
    32461 gatggccttg ccgtcagtat tctgtatgaa aatggcgttt tagtcagtgc cgcgacccgt
    32521 ggcgatggca ccaccgggga agatatcacg tctaatgtgc gtactattcg cgctattccg
    32581 ctgaagctgc acggagagaa tatcccggcg cgtctggaag tgcgtggtga agtgttcctg
    32641 ccgcaggcgg ggttcgaaaa gattaacgaa gatgcgcgac gcacgggcgg gaaagttttt
    32701 gctaacccac gtaatgcggc agctggttca ctgcgtcagc ttgatccgcg tattacagcg
    32761 aagcgaccgc tcactttttt ctgctatggc gttggtgttc tggaaggtgg cgagctgccg
    32821 gatactcatc ttggccgttt actgcaattt aaaaagtggg ggttgccggt cagcgatcgg
    32881 gtaacgcttt gtgaatcggc ggaagaagtg ctggcgttct atcacaaagt ggaagaagac
    32941 cgcccgacgc tgggctttga tatcgacggc gtggtgatta aggtcaactc actggaacag
    33001 caggagcagc ttggctttgt cgcgcgtgcc ccgcgctggg cggtagcgtt taaattcccg
    33061 gcgcaggagc agatgacctt tgtgcgtgac gtcgagtttc aggttgggcg tactggcgcg
    33121 attacgcctg ttgcgcgtct ggaacctgtc catgttgctg gcgtgctggt gagtaacgca
    33181 accttacaca atgcggatga aatcgaacgt cttggtttac gcattggcga taaagtggtg
    33241 attcgccgcg ctggcgacgt gatcccgcag gtggttaacg tcgtgctttc tgaacgcccg
    33301 gaagataccc gtgaggttgt attcccgacg cattgtccgg tatgtggttc tgacgttgag
    33361 cgtgtggaag gtgaagcggt tgcccgctgt accggtggcc tgatttgcgg tgcgcagcgt
    33421 aaagagtcgc tgaaacactt tgtttcccgc cgtgcgatgg atgttgacgg aatgggcgac
    33481 aaaatcatcg atcagctggt tgaaaaagaa tatgtccata ctccggcaga tctgttcaaa
    33541 ctcaccgcag gcaaactgac cggactggag cgtatggggc caaaatcggc acaaaacgtg
    33601 gttaacgcgc tggaaaaagc gaaagaaacc acctttgctc gcttcctcta tgcacttggc
    33661 atccgtgaag tcggcgaggc caccgcagca ggtctggcgg catatttcgg cacgctggaa
    33721 gcgctggaag ccgcttcgat tgaagagctg caaaaggtgc ctgatgttgg cattgtcgtt
    33781 gcatcccacg ttcacaactt ctttgccgaa gaaagcaacc gcaatgtcat cagcgagctg
    33841 ttggcggaag gtgttcactg gcctgcgccg atcgttatca acgcggaaga gattgacagc
    33901 ccgtttgctg gtaaaaccgt ggtgcttacg ggcagcttaa gccagatgtc gcgtgatgac
    33961 gctaaagctc gactggttga actgggcgcg aaagtcgcgg gcagcgtgtc gaagaaaacc
    34021 gatctggtga ttgccggaga agcggcaggt tctaaactgg cgaaagcgca ggaactgggc
    34081 attgaagtca tcgacgaagc ggaaatgctg cgtttgctgg gtagctgaga tggaaaaaga
    34141 gcagctgatt gaaatagcca atacgataat gccgtttggc aaatataaag ggcgtcgctt
    34201 aatcgacctg ccggaggagt atctgctgtg gtttgcccgc aaagatgaat tcccggcagg
    34261 gaagctcggt gagctaatgc aaatcacgct gctgattaaa accgaggggc tgacgcaact
    34321 ggtccagccc ctgaagcgtc cgctttaagc tttatcggcg ctgctttcct gctgtgcctg
    34381 taactgttcg gtctggcgtt tgtatcgacg cgccagcacc gcacagacca tcaattggat
    34441 ctgatggaaa atcattaggg gcagcaccat cataccgatc accgatgtgg ggaacagaat
    34501 gtttgccatc gggatgccat ttgccagact ctttttcgaa ccacaaaaga cgatagtaat
    34561 ttcatctgcc ttattgaagc ccagtcggcg tgccataaag acgttaacta caatcacgat
    34621 agccagaaga acgcagctga ccaccacgat aaacagcaat gatccccagc caactttatg
    34681 ccagatacca ttaacgacgg cttcgctgaa tgctgtataa accaccaaca gaatggacgt
    34741 ctggtcagtt ttcgcaatcc attttttatt gcgcgacacc cagtcaccaa tccacggccg
    34801 ggaaagatgc cccaacacaa acggcagcag cagttgcagc ataattttac cgacctgctc
    34861 aaggctgccc cctgcaccgt gaacattcat caccagacca accagcaatg gtgaaaggaa
    34921 aatccccagc aggctggatg ctgacgcaga acaaaccgcc gccgcgacgt taccgcccgc
    34981 cattgacgtg aaggcgattg cagactgcac ggtagccggg agaatgcaca agtagagaaa
    35041 accggagtag agcatcgggt cgacatttac cggtttccac caggcaaaca gtacacccag
    35101 aatcggaaac agcacgaagg tgctgcaaat tacccacaaa tgcagtcgcc agtgaccacc
    35161 gccagcaata atcgcctcac gcgacaactt cgcgccgtgc ataaagaaca gcagggcaat
    35221 cgctgcggtg gtcagatttt caaagaaggg gacgaaatcg cctctggccg gaaagaaaga
    35281 ggccagcaac accaccgtga tcagggttaa ggtgaaagga tcgaggatac gaaaaagttt
    35341 cataaagact ccagatgatc gatggttcta ttgtgcttct tccagtttga gaaataaaat
    35401 tgatttattg catccattca tgaagaaaac agatgaatta ttctttaaaa caattaaagg
    35461 ttttcgtcac agtagcgcag gagaaaagtt ttagtcgtgc aggagagcgt atcggcctga
    35521 gccagtcggc agtgagtcac agtgtgaagg aactggaaaa tcatactggt gttcgcctgc
    35581 tggacagaac cacgcgtgaa gtggtgctta cagatgcagg gcagcagctg gctttgcgtc
    35641 ttgagcgact gctggatgaa ctgaacagca cgttgcgcga taccgggcgt atggggcaac
    35701 aactgagcgg aaaagttcgg gtcgctgcca gccagaccat ttccgcgcat cttattccgc
    35761 aatgcattgc tgaaagccat cgccgctatc cagatattca gtttgtcctg cacgatcgtc
    35821 cgcagcagtg ggtgatggaa agtattcgcc agggagatgt cgactttggc atcgtcatcg
    35881 atccaggccc agtgggcgat ctgcaatgtg aagcgattct ttccgagcct ttctttctgc
    35941 tttgccatcg cgatagcgct ttggccgtcg aagattatgt accctggcag gcattacagg
    36001 gagcgaaatt ggtgttgcag gattacgcgt caggcagccg accgctgatt gacgcagcgc
    36061 tggcgcgcaa tggtattcag gcgaatattg tgcaggagat tggacatccg gcgacgctgt
    36121 tcccgatggt agccgcaggc atcggcatca gtattcttcc cgcactggcg ctaccattgc
    36181 ctgaaggtag cccactggtg gtaaagcgca ttacgccggt tgttgaacgc cagttgatgc
    36241 tggtgcgcag gaagaaccgt tcactttcca ccgccgctga agcgttatgg gacgtggtac
    36301 gagaccaggg caatgcgcta atggctggtc gggaagggga tccgttgtat cagatataga
    36361 gtgaattcat actgccggga gccccggcag taatcaatca gaagttaaat ttgatggagt
    36421 agattatcgc atcctggtaa aaatcttcgc ccagtttgtc atccgcatat cgatactgaa
    36481 tccccgtagt aatgtgtgat gttgcattcc accacagtgc aactgcgcca ttcagccctt
    36541 ctttaccgcc attacccgct gcataggtag cgtcacggtc aaactcgtac tcgttccagt
    36601 tggtaagagt gaatttctcg ctgcccagca taaagttgta acctgcaacc cagccggcga
    36661 catagccgtt gtcgccagtg taataggttt gatctgtata acgcttagca aagaacggtt
    36721 tgaaccacca accgctgccg gtgaaattgt agccgatacc gtacaggaac atatcgtcgt
    36781 ggaaattcac gcgattcgca gaaccgtagg tgccatacgc atgcaagtag agattaaatc
    36841 cggtgtcacc caggtaaata cggttggtat ttttaaaggt ataacgctgc tcgctacctg
    36901 gtttattatg gcgaccgtta tagaagtttt cccagtcgaa gaacccgtac atttctcccc
    36961 aactaaagtt agcgccacct tcaaattcaa gataaccaaa atcatctttg tgtgatttgg
    37021 tcgacgattt ttcggtagtc cggctggtcc agtccagata atgcacgccg atatctgcaa
    37081 aaccgccttt aaattctgca tgagatacaa caggtatcgc taatatagag gaaagtgtca
    37141 gagttaaaag atgttttttc atagcataat tccctatgcc gatcgttatt agtagattgc
    37201 ttaataaagt ggtttgctaa aaacgacata ttgttaatgc aaaggaatta atatcgccaa
    37261 tacagttttt accgtggatt aatagagaaa agatataaat ccatcgatat cgaatcgcta
    37321 ttgaatgcgt gagaaaagtg tattggtaag agccgggaca acctcgctat aaaaaggcgt
    37381 tagattccac cctacagaaa aaggatatgt atgtctcagg ttcaattttc tcataacccg
    37441 ctgttttgca tagatattat caagacttat aaacctgatt tcacgccacg agtggccttt
    37501 attttaggtt ccgggctcgg cgcgctggcc gatcagattg agaacgctgt cgcaatttcc
    37561 tacgaaaagc tgccgggctt cccggtaagt acggtacatg gtcatgcggg ggagttagtg
    37621 ctgggccatc tccagggcgt accggtggta tgtatgaaag gtcgcggaca tttctacgaa
    37681 ggtcgtggaa tgaccattat gactgacgca attcgtacct ttaagctgct gggctgcgag
    37741 ctactgttct gcaccaatgc ggcaggctcg ctgcgtccgg aagtgggggc aggcagcctg
    37801 gtcgcattga aagatcatat caacaccatg cccggtacgc cgatggtggg tcttaacgat
    37861 gatcgttttg gagagcgctt cttctcactg gcaaatgcct acgatgcgga ataccgcgca
    37921 ctgttacaaa aagtggcgaa agaagagggt ttccctctga cggagggcgt gttcgtctcg
    37981 tatccggggc cgaatttcga gactgcggcg gaaattcgca tgatgcaaat tattggtggg
    38041 gatgttgttg gtatgtctgt ggtgcctgag gttatttcag ctcgccattg cgaccttaaa
    38101 gtcgttgcgg tctctgcgat taccaatatg gcggaaggtt taagcgatgt gaaattgtca
    38161 catgcgcaaa cgctggcggc tgcggagctc tctaagcaga acttcatcaa ccttatttgt
    38221 ggctttctgc gcaaaattgc ctgagaaaaa caataagaaa cggcctcgcg ggtgaggcca
    38281 aatgatgcga caaggaaacg attatgagca tcgcgatgcg cttaaaggta atgtcctttt
    38341 tgcaatattt tatctggggg agctggctgg ttaccctcgg ctcttacatg attaataccc
    38401 ttcatttcac cggcgctaat gttggcatgg tttacagttc caaagggatc gccgcgatta
    38461 ttatgcctgg tataatgggg atcatcgcag acaaatggct gcgcgcagaa cgtgcataca
    38521 tgctgtgtca cctggtgtgt gcgggcgtac ttttttatgc ggcatccgta actgatccgg
    38581 atatgatgtt ttgggtgatg ttagtcaatg cgatggcgtt tatgccgact attgcgttat
    38641 cgaacagcgt ctcttattcc tgtcttgccc aggcagggct tgacccggtg accgctttcc
    38701 cgcccattcg cgtttttggt acggtggggt tcattgtcgc gatgtgggca gtaagcctgc
    38761 tgcatctgga attgagtagt ctgcagctgt atatcgcgtc cggtgcgtca ttgctgctgt
    38821 cggcttatgc gctgactttg ccgaagattc cggttgcgga gaaaaaagcg accacatcgc
    38881 ttgccagcaa gctgggtctg gatgccttcg tgctgtttaa aaatccacgc atggccatct
    38941 ttttcctctt tgccatgatg ctgggtgcgg tactgcaaat taccaacgtt tttggtaatc
    39001 cgttcctaca tgatttcgcc cgtaacccgg agtttgctga cagttttgtg gtgaaatatc
    39061 cctccatttt actgtcagtt tcacagatgg cagaagtggg ctttatactg actatcccat
    39121 tctttttaaa gcgatttggc attaaaaccg tcatgctgat gagtatggtg gcctggacgc
    39181 tgcgctttgg cttcttcgcc tatggcgatc cgtcaacaac cggatttatt ttgctgctgc
    39241 tgtcgatgat tgtttatggc tgtgcattcg atttcttcaa tatttctggt tcggtatttg
    39301 tcgaacagga agttgattcc agcattcgtg ccagcgcgca ggggctcttt atgaccatgg
    39361 taaatggtgt cggcgcatgg gttggctcga ttctgagtgg catggcagta gattactttt
    39421 cggtggatgg cgtaaaagac tggcaaacta tctggctggt gtttgcagga tatgctcttt
    39481 ttctcgcagt gatatttttc tttgggttta aatataatca tgaccctgaa aagataaagc
    39541 atcgagcggt ggctcattaa aaggattcgc agctctgctc ttcagagctg cttttatgat
    39601 aaaggttaat tagtgaaaga tattttattt tcttccggcg ttggttttgg cattggtgct
    39661 ttgtttacaa tcgtgcgctt gcctattcct gtaccaaatg tattaccagg tatattatcc
    39721 attgtgttta tgtatgtcgg atatctggtg gtgaaatact ttatgccatg ataatttaat
    39781 acgatgtatt tattatatgg agcacttaat tatggaacgc gtatacagaa cagatcttaa
    39841 gttgctccgt tattttcttg ccgtagcgga agagttgcat tttggccgcg cagcagcgcg
    39901 tttaaatatg tctcagcctc cgctcagtat tcatattaaa gagctggaaa atcaactcgg
    39961 cacgcagctt tttattcgcc attcgcgcag cgtcgtactg acacacgcgg gcaaaatctt
    40021 gatggaagaa tcgcgtcgat tgctggtgaa tgctaataat gtattggctc gggttgaaca
    40081 aataggtcgg ggagaagcag ggcggattga actcggcgta gtgggaacgg ctatgtgggg
    40141 acggatgcgc ccggttatgc ggcgattcct cagggaaaat cctaacgttg aagttctttt
    40201 tcgcgaaaag atgcccgcga tgcaaatggc cctgctggaa cgacgcgaac tcgatgccgg
    40261 gatctggcga atggcgacag aaccaccgac aggttttacc agcttacggt tgcatgaatc
    40321 ggcgtttctg gtggcgatgc ctgaagagca tcatctctca tcattttcca ccgttccgct
    40381 ggaagcgcta cgtgacgagt attttgttac aatgccgccc gtgtacactg actgggattt
    40441 tttgcagcga gtttgccagc aggtgggatt ttcaccggtt gttattcgcg aagttaatga
    40501 accgcaaacg gtactcgcca tggtcagtat gggtattggt atcacattga tagcggacag
    40561 ctacgcacaa atgaactggc caggtgtcat tttccgtccg ctcaaacagc gcatccctgc
    40621 agatttatat attgtttatg aaacacagca ggtgacgccc gcgatggtta agctgttggc
    40681 tgcattgaca cagtaggtga ggagacagga attgccggaa atagtcgtct gctaagtgaa
    40741 gcgcatcagg caatttcgca
//
LOCUS       DAZGLJ010000018        40749 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000018 DAZGLJ010000000
VERSION     DAZGLJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.018"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(119..361)
                     /gene="yiiF"
                     /locus_tag="ACHK49_000876"
     CDS             complement(119..361)
                     /gene="yiiF"
                     /locus_tag="ACHK49_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFO9261564.1"
                     /translation="MNSLAGIDMGRILLNLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(580..798)
                     /gene="yiiE"
                     /locus_tag="ACHK49_000877"
     CDS             complement(580..798)
                     /gene="yiiE"
                     /locus_tag="ACHK49_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO9261565.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(1403..1693)
                     /gene="nadS"
                     /locus_tag="ACHK49_000878"
     CDS             complement(1403..1693)
                     /gene="nadS"
                     /locus_tag="ACHK49_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO9261566.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(1694..2005)
                     /locus_tag="ACHK49_000879"
     CDS             complement(1694..2005)
                     /locus_tag="ACHK49_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9261567.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            2234..3142
                     /locus_tag="ACHK49_000880"
     CDS             2234..3142
                     /locus_tag="ACHK49_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9261568.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(3206..4147)
                     /gene="fabY"
                     /locus_tag="ACHK49_000881"
                     /gene_synonym="yiiD"
     CDS             complement(3206..4147)
                     /gene="fabY"
                     /locus_tag="ACHK49_000881"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO9261569.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(4192..4629)
                     /gene="dtd"
                     /locus_tag="ACHK49_000882"
     CDS             complement(4192..4629)
                     /gene="dtd"
                     /locus_tag="ACHK49_000882"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO9261570.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(4626..5498)
                     /gene="yihY"
                     /locus_tag="ACHK49_000883"
     CDS             complement(4626..5498)
                     /gene="yihY"
                     /locus_tag="ACHK49_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO9261571.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(5492..6091)
                     /gene="yihX"
                     /locus_tag="ACHK49_000884"
     CDS             complement(5492..6091)
                     /gene="yihX"
                     /locus_tag="ACHK49_000884"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO9261572.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(6190..6975)
                     /gene="csqR"
                     /locus_tag="ACHK49_000885"
     CDS             complement(6190..6975)
                     /gene="csqR"
                     /locus_tag="ACHK49_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO9261573.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(7009..7905)
                     /gene="yihV"
                     /locus_tag="ACHK49_000886"
     CDS             complement(7009..7905)
                     /gene="yihV"
                     /locus_tag="ACHK49_000886"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFO9261574.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            8073..8969
                     /gene="yihU"
                     /locus_tag="ACHK49_000887"
     CDS             8073..8969
                     /gene="yihU"
                     /locus_tag="ACHK49_000887"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO9261575.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            8993..9871
                     /gene="yihT"
                     /locus_tag="ACHK49_000888"
     CDS             8993..9871
                     /gene="yihT"
                     /locus_tag="ACHK49_000888"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFO9261576.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            9888..11129
                     /gene="yihS"
                     /locus_tag="ACHK49_000889"
     CDS             9888..11129
                     /gene="yihS"
                     /locus_tag="ACHK49_000889"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFO9261577.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            11267..12169
                     /gene="yihR"
                     /locus_tag="ACHK49_000890"
     CDS             11267..12169
                     /gene="yihR"
                     /locus_tag="ACHK49_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFO9261578.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            12368..14404
                     /gene="yihQ"
                     /locus_tag="ACHK49_000891"
     CDS             12368..14404
                     /gene="yihQ"
                     /locus_tag="ACHK49_000891"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFO9261579.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDNDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            14450..15835
                     /gene="yihP"
                     /locus_tag="ACHK49_000892"
     CDS             14450..15835
                     /gene="yihP"
                     /locus_tag="ACHK49_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9261580.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            15878..17281
                     /gene="yihO"
                     /locus_tag="ACHK49_000893"
     CDS             15878..17281
                     /gene="yihO"
                     /locus_tag="ACHK49_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9261581.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            17349..18041
                     /gene="ompL"
                     /locus_tag="ACHK49_000894"
     CDS             17349..18041
                     /gene="ompL"
                     /locus_tag="ACHK49_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFO9261582.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(18132..19397)
                     /gene="yihN"
                     /locus_tag="ACHK49_000895"
     CDS             complement(18132..19397)
                     /gene="yihN"
                     /locus_tag="ACHK49_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9261583.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(19499..20479)
                     /gene="yihM"
                     /locus_tag="ACHK49_000896"
     CDS             complement(19499..20479)
                     /gene="yihM"
                     /locus_tag="ACHK49_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO9261584.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(20487..21197)
                     /gene="yihL"
                     /locus_tag="ACHK49_000897"
     CDS             complement(20487..21197)
                     /gene="yihL"
                     /locus_tag="ACHK49_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO9261585.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPKTLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(21414..23237)
                     /gene="typA"
                     /locus_tag="ACHK49_000898"
     CDS             complement(21414..23237)
                     /gene="typA"
                     /locus_tag="ACHK49_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO9261586.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            23610..25019
                     /gene="glnA"
                     /locus_tag="ACHK49_000899"
     CDS             23610..25019
                     /gene="glnA"
                     /locus_tag="ACHK49_000899"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO9261587.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            25338..26387
                     /gene="glnL"
                     /locus_tag="ACHK49_000900"
     CDS             25338..26387
                     /gene="glnL"
                     /locus_tag="ACHK49_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO9261588.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            26399..27808
                     /gene="glnG"
                     /locus_tag="ACHK49_000901"
     CDS             26399..27808
                     /gene="glnG"
                     /locus_tag="ACHK49_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO9261589.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            27920..28030
                     /gene="yshB"
                     /locus_tag="ACHK49_000902"
     CDS             27920..28030
                     /gene="yshB"
                     /locus_tag="ACHK49_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO9261590.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(28220..29593)
                     /gene="hemN"
                     /locus_tag="ACHK49_000903"
     CDS             complement(28220..29593)
                     /gene="hemN"
                     /locus_tag="ACHK49_000903"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO9261591.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKLW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(29782..30291)
                     /gene="yihI"
                     /locus_tag="ACHK49_000904"
     CDS             complement(29782..30291)
                     /gene="yihI"
                     /locus_tag="ACHK49_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO9261592.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(30410..30576)
                     /locus_tag="ACHK49_000905"
                     /pseudo
     CDS             complement(30410..30576)
                     /locus_tag="ACHK49_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            30873..31505
                     /gene="yihA"
                     /locus_tag="ACHK49_000906"
     CDS             30873..31505
                     /gene="yihA"
                     /locus_tag="ACHK49_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO9261593.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(31887..34673)
                     /gene="polA"
                     /locus_tag="ACHK49_000907"
     CDS             complement(31887..34673)
                     /gene="polA"
                     /locus_tag="ACHK49_000907"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO9261594.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPNDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFSLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            35037..35969
                     /gene="yihG"
                     /locus_tag="ACHK49_000908"
     CDS             35037..35969
                     /gene="yihG"
                     /locus_tag="ACHK49_000908"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO9261595.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDQLLTSLMSSQRQDK"
     gene            complement(36010..37440)
                     /gene="yihF"
                     /locus_tag="ACHK49_000909"
     CDS             complement(36010..37440)
                     /gene="yihF"
                     /locus_tag="ACHK49_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO9261596.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(37595..38221)
                     /gene="dsbA"
                     /locus_tag="ACHK49_000910"
     CDS             complement(37595..38221)
                     /gene="dsbA"
                     /locus_tag="ACHK49_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO9261597.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(38238..39224)
                     /gene="srkA"
                     /locus_tag="ACHK49_000911"
     CDS             complement(38238..39224)
                     /gene="srkA"
                     /locus_tag="ACHK49_000911"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO9261598.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(39301..39570)
                     /gene="yihD"
                     /locus_tag="ACHK49_000912"
     CDS             complement(39301..39570)
                     /gene="yihD"
                     /locus_tag="ACHK49_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO9261599.1"
                     /translation="MKSKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            39640..40224
                     /gene="mobA"
                     /locus_tag="ACHK49_000913"
     CDS             39640..40224
                     /gene="mobA"
                     /locus_tag="ACHK49_000913"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO9261600.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            40206..40733
                     /gene="mobB"
                     /locus_tag="ACHK49_000914"
     CDS             40206..40733
                     /gene="mobB"
                     /locus_tag="ACHK49_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO9261601.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT        10068 a        10334 c         9839 g        10508 t
ORIGIN      
        1 aataaacttc aatatattga atttgcatga ctttgtaggc cggataaggc gttcacgccg
       61 catccggcat gaacaacgcg cacgttgtca gcaatctgat tgacatcatt ttttcccatt
      121 accattcctc gcgcagctta cgctgatatt cgacaccatc ttcctcacac ccttgccaga
      181 tgccaggaac actggttctt gctgtttgcg attgatttat caggtattga tctaccgctt
      241 cccttaatag gtccgcacga ggaagattac gctgcacctc gagatcatca agttgcttaa
      301 tcacctcatt cgataaattg agtaaaattc tgcccatatc tatacctgcc agagagttca
      361 taaatatctc gccaatatca tttttgaatc tatggagaga aaagtaccct tgccgaatct
      421 ttaaagaaag ctcatttacg catcattttt tatttttgcg aggcgttttc cagatacgat
      481 gtaacagttg cagttattat aagttcaagt aaaacagcct tgtattaata ccagaataag
      541 ggcatctgtt tttttatatt caagaatgaa aaattttttt cattccttat gctccttaca
      601 ctgatcatca acaccaacta cctccccctt ttgcctgcct ccctttgctt tacgcaccgg
      661 aaattgctgc cgctccagat attgctcaac cgcttcgcgt aaaattgcag atcgtgaaac
      721 cttacgccag ccccgaagtt tgttcagccg tttaattgcc tcttctgata aatgaagaaa
      781 aaccgtattc atcgccatta ccaccacctt acctatatta ctcattaatg tatgtgcgaa
      841 aattacgccg gaaaatcatt taaaaaaagc ggatttttgc atgttgtttt gtttttgcga
      901 ggcgttttcc agaaataata agagtgttgc agttgtttca tttattactc aaaaatggtt
      961 tagttatcgc ccaggatgag ttatggatgc tatgaatata ttatattcag ggatgtttat
     1021 tttttgattg gatctttatt tgcataatct gaatggtgta cccgaaaaat gtcagcatcc
     1081 taacagcacc cggatttaag gtgaaattaa tcatccatca cagaccggtc agtactgcca
     1141 ccaaccggtc tttacttgtt catcgttata attttgttgg taacagttcg ggaaacaagg
     1201 aattccttcc ggaacccccc ggtatcatcc gatagcattg acgtcaatca tcagcgcgac
     1261 gatgagtttt tttaatcgca tcatagggtg gcgacgttca gcacgattac gggtcatagc
     1321 aaaactcctg ggcaggcgac cactatgccg ctttcacagg agttctgaat gctagtgctt
     1381 tctgtatggc ataaccatgc ctctaacggt taagtgcttg cagcacatgt tgcggatcat
     1441 tggcaatcgc tcgcagcaaa gcctttgcag gcccggtcgg tgaacgtcgc ccctgctccc
     1501 agttacgcaa cgtgtctacg cttacagaaa taagatcggc aaatttagat tgtgacaacc
     1561 ctgaagcgcg gcgtatttct ttgattttca tagcatcaac ttcaaaagtc cgcgaaggct
     1621 gtcgcgttcc ttcaataatt tcattcatct gggccatgct gtgagtcaac cgctcaaaaa
     1681 gtgccttatc catttaccac ctcgtattta acaaccggag cattgctttt tcctggggag
     1741 acagatcatc tttaataccc ttacgataaa taagcagtaa tctgatctca taatgtttaa
     1801 cctgatgaaa gtaaataacc ctgacaccag cacgtttccc ctttcctcct gacacccatc
     1861 gaacctttct taacccacca gtttcaggta ttacctcacc atagtcagga ttcaaggcca
     1921 ggaaaaactg aaaacgactg aattcatcgt cggtcagcag tttttgaaca tcttcagtaa
     1981 aaatttccgt ttcgatgaag agcataatta tacgccattg gcttagagta acaatccttt
     2041 ttgtacgtca ttgacgtacc cttgaaacca ctagcttact gatagtgctc cagcaaaacc
     2101 acgtctctgc cgggtttgtc tttagatttg cgaggcgctt tccagatttt tcacgcccgt
     2161 gaatttccct gtatccatcc agcaaaaacg ggtgtataag actgtacatt catacagcaa
     2221 aataagttct actatggcac tggaaaaagg aattgcagag ttagtcgaaa agtttatcgc
     2281 tgcgggtcgc ccctcatcgc gtcaacaaag tattgcgcag cgcagggagg gctatatcgc
     2341 cagcgctgtc cttgcgggtg agacagaaac gcgggtagat attcagacta tcgaacttga
     2401 agggatgacc ttacgaatag tgtcgccgct gaatgccccg acattactcc cgacgataat
     2461 ttattactac ggtggctgtt ttgttagcgg cgggttcgca actcacgaca accagctgcg
     2521 ccagttagcc tattacgggc aatgtcgggt tattgccgtc cagtatcggc tggcaccgga
     2581 acatactttc cccgctgccc atgatgatgc gcaacgaggt gcagaaatag tctggcaaca
     2641 cgccgaacga ctcggcgttg ataaacaacg tataactctt gccggtgaca gtgcgggcgg
     2701 acatctggcg ctggtaactg ctttgcgatt aaagcgtgct ggcgagtggc aaccggcaca
     2761 actcatctta atttatccta tgcttgatgc gactgcgcat ttcgaaagct acatacgcaa
     2821 tggtcacgat tacatcatca cacgcgacac tttactgagc ggctttgaga tgtatctgcc
     2881 aggcattgaa cgcaggcatc ctgaagccag ccctctttgg cgcgatgatt tcaatggctt
     2941 gccacctgta catatcatta ccgctgaata cgacccactt tgcgatgaag gtgaagcgtt
     3001 gtatcaccgc atgacagggc aaggagtgca atgcacctgc caacgctacc ttggcgttat
     3061 ccacggattt tttcagttag gtggtatcag tgaggccgcg cgatcggcac tacgggatgt
     3121 tgcctggaga gcgggaaggt aacttcggac ctgggccctg aaaaggtttt cagattgaaa
     3181 accttttcag tcaagtaaaa cgcccctact cttcttcgtt cccgccctct tcatacgggc
     3241 caaatggctt cgcgggcaga acgatatacg tgccttcaaa cactgcaccc ggcgtctcgt
     3301 cgccaaagat ttcaacttgc atctgcaccc gtgcttttcg tccgcgcgcc agacggtcga
     3361 gatcgccgct taaggcacca aggtcggcta ccgcatgagg tttaccgcta atcggcttgc
     3421 tgtagcggat atgcgcatcc gccagaataa tcgttccgcc gagatggcgt tcgcgcagca
     3481 tcagccagat aagcccccaa ccggtgagcg ttgccagtga gaataaactc ccggcaaaca
     3541 gcgtatggtg cggattctga ttaccggttt ccggcatggt ggtgataaat ttttgcccgg
     3601 tatattgttg aatacgcacg cccatttttt cactaagcgg gatatgttcg taccacgcct
     3661 gttgcagctg cgcgcaccag tcgccgcgat gcaaaatatc atccagagtg gcgacgggct
     3721 taatcatcaa aaaatggcga atcggcgtgg tggttggcgt ggtgatttct ccctgattaa
     3781 caaaccccag cttggcgaaa aactccaccg cgtcttcacg ggcgctacag gtcacgcgct
     3841 taacgccttc ctgacgcgcc accgactcca gggtcatcgc catcagcgtt cctaaccctt
     3901 tgtcctgcac gtccgggtga acggccataa agcgaatgga cgcttcattg tcggcattaa
     3961 tatacaatcg gcctaccgcc accagattac cctgctcgtc gacgaccatc tggtgatgcg
     4021 ccatcgcatc ccacgcgtcg cgttccgaac cttttggttg atgcaggggc ttacgcaaca
     4081 tttcccagcg aaactgatag taacgctcta actcttcttc tgtttgtgga acccgaaggt
     4141 gatacatagc gatactctct cttgtttccc gtgacaaccc tggaagctgg ctcatacctg
     4201 caaccagaat gtcacggggc catcattgac cagcgatacc tgcatatcgg cagcgaagcg
     4261 tcctgtttgc gtgttcatct cttgctggcg gcagcgttcg acgaaataat catataacgc
     4321 ctctgcgcga tccggtgatg cgcctttaga gaaacttggg cgcatccccc gttcggtatc
     4381 tgcggcgagg gtaaactggg aaaccaccag tacactgccg cccgcctgtt gcacgttgag
     4441 attcatcttg ccttcggcat cgctaaagat gcggtagccg agcacacgct cgcacagacg
     4501 gtttgctttc tgttcgtcgt catccttttc gacacccaat aacaccaaaa gtcccgcgcc
     4561 aatttcgccc gtcacttctc cctccacggt gacgctggca cgggttacgc gttgaattaa
     4621 tgcaatcatg gttcgtcgtc ttcttcttgt tcagctgctt gttttagttt gcggtattcc
     4681 ccgagagtga cagtaatttc cgcgccaagc aagacgatac accatgtcca gtagacccaa
     4741 acaaagagaa tggggatcac cgccaacacg ccgtaaatga gctgatatga cgggaacatg
     4801 gtgatataaa gcgcgaaacc tttctttcct gcttcgaaca ggagcgcggc gacaaacgcg
     4861 ccgacaatcg cgtcgcggtt aggtacgcgg atggtaggaa caatgctgta cagcaaccag
     4921 aaggagatcc acgacaacag cagcggaaaa atacgcagca cgttatcgat gacagtattg
     4981 agatcgctcg cccagcgcag ggagagcaaa taggaactga tcgccagact ggcccctgcc
     5041 agcagcggcc ccagcgttaa aatcatccag tacacggcga acgagtaaat ttttggtcgc
     5101 gctcgtttac tgcgccagat ggtattcaac gcgctatcga tggagtacat cagcaataac
     5161 gcggtgacga tcagcccgca cgccccaacg gcggtcatct tgttggaatt ggcaacaaat
     5221 tgttcgatat accgctgaat aacatcgcca gtagcaggca gaaagttggc aaaaataaag
     5281 tgacgtaact gaatgctgac gtcggaaaac atgggaaaag cggcgaaaag cgcaaaaaca
     5341 acggcaacca gcggcactaa tgagagcaac gacacatagg caaggttacc tgccagggtt
     5401 gtcatgttgt cctcatcaat acgttgccag agtagtttta gccaggccca tagtggacgg
     5461 gtacggtgcc tggctttgtc ctgaatggtt tttagcataa caccttcgcg aaatagtccg
     5521 ggatggtggt tttatctttc accagaatac tggtaatgcc cagctgattg gctccttcta
     5581 tattatcggc gttatcgtcg aaaaagaccg tatcgctggg tgaaaaacct tccgcctgca
     5641 aaacatgctg gtaaattcgt gcttcaggtt tgcgcatccc cagatcttgc gacagataga
     5701 tatggtcagc agcatcacga atttccgggt attcttccgg ccagaaggtg gtatgcaggc
     5761 ggttagtatt ggaaagcacc accacgcgat gcccctgctc acgcagttta tgcatgatgg
     5821 cgatcacttc cgggcgcagc gcaacaaaca ccgcctgcca gccgtgagag aactgctcgt
     5881 agcttagcgg cagggccatc tcatgacaca gcgcctctgc gaacgcttcg tcgctaattt
     5941 ccccacgctc atgctgatga aacgcctccc ccatgtggaa actcttctta agcgtagcca
     6001 gtggaacacg cgttaaatcg ctccaggctc ccagcacacg gttaaagtcg atatcgacaa
     6061 tcacattacc taaatcaaag atatagagca tttttgcctc ctttcgcgcc acaagaaatt
     6121 aactgtagcg ggaaaggatg ggtttggcta tgtggcaagg tatgaagcca ggttaacggt
     6181 cacaaaaatc tacgcgtctt cctggggaag aatgatttca atttggctgt cttgtaagcg
     6241 tgattttagc gcagcgggcg gcagctcgtc ggtaaagagc gcagtgacct gtgccacgtt
     6301 accaatttca accgccgccg aagcatgata cttagtgtga tcggcgacca gcagaatatt
     6361 tctcgcgtgc gccatcatcg ttttcaccac gttagcttcg tttacatcaa actccatcaa
     6421 cgcgccatcg ctctcaatcg ccccaacgct tgttaccaga taatcagcac gaaaatcagc
     6481 cacaaaggac gccgctgaag ggccaatgat cccgctatta tgagagcgca acgtaccgcc
     6541 gggcaccatc acttcaaagc gcgggttgtg gtaaagaata tgcgccacac gcaggctgtt
     6601 ggtgattatc cgcaaatgat tatggttaag taacgcccgg gcaacatgct caacagtcgt
     6661 accaatggtg ataaatattg ttgaaccatc aggaatatag tctgccaccg cttcggcaat
     6721 cgcttttttt tcctcggttt gcgaaacctc acgctgctcg aacgccgtat taacgacgct
     6781 ggaagcccga cccgcgccac cgtgatggcg cgtaatcagg ccttgctcgc ttaatttacg
     6841 aatatcccgg cggaccgtct gcgtggagac atccagcaga tttgccagct catcaatatt
     6901 catataccca cgctcggcga tcagcatgag gagttggtcg tgccgcgggt taccggttag
     6961 ttcggtaagg ctcatgaaaa ttcctcgaaa aaccatcacc ctggcatttt atacaaaaag
     7021 tgacaaaaaa gatcgggttt gatcacagtc agggatcccg gctcgtccac cgggacgtgt
     7081 gcattttaac gccgctacac cgctggcgaa gcggactgac tccgctaaat ccccacttgt
     7141 tgccagcgcc accgccaaag cgccgtgaaa aacatcaccc gcacctgtgg tatctaccac
     7201 atcaactttg aaggccggtt gatgctgacg cccaccattt tccagccagt cgcagcctgc
     7261 gctaccctgg gtcacataga catgaccatt tgtgagcgtt tgtgcctgtt ttagcgcact
     7321 ggccatctct ttcacgcccg ttaagcgcgc cagacccggt tctgaaaagg ccgcgtgatc
     7381 gcttaatgcc accagctcac tgatatcctg cggcgtaatg tccccgtcca gaacggtcat
     7441 cacacccgcc tgacgggcca gggtgaaggc ttttttagcg ccgtcgtgcc agcgtacatc
     7501 tgccagcaca acatcccact gagagaaatc aatttcctcc aaccactctg cgtcaggcag
     7561 cagatccggg ctggggtagt taatgattat ccgctcgcct ttggtatcca ccatgatggc
     7621 ggattgcgaa gatttcgcct ggttataccg tttggtgtaa cgggtgttaa cgccccagga
     7681 ttccagctct gccagcaggc tgttgcccgt gtcgtcatca cctacgcgac caataaaatc
     7741 gacctgcgcc cccagccttg ccgccgcaac cgctgccgtc gccgctggcc cgccaccaac
     7801 ttccgtataa tttctcgcca cgtatttacc gctctccgtc ggtaaccctt ccacgtaata
     7861 gatgcgatcc atcacggtta tacctacaca agcaacacga atcatggtca ttccttaaac
     7921 attttgggat gcgttcattt taatttcacc aattgtttaa aaagtgacgg ctgtcaattt
     7981 tttgacgata aattacaaat acgatcaaaa acagacaagc aaagcatgtc ataaatgaca
     8041 aaaagtgaca tcactgtatt caggagaggg ttatggcagc aatcgcgttt atcggtttag
     8101 gacaaatggg ttcgccaatg gcgagcaatt tattgcagca agggcatcaa cttcgcgtct
     8161 ttgatgtgaa cgccgaagct gtgcggcatc tggtagacaa aggtgcgact cccgccgcca
     8221 acccggcgca ggccgctaaa gatgccgaat ttatcattac catgttgccg aatggcgatc
     8281 tggtgcgcaa cgtgttgttc ggtgaaaacg gcgtttgcga aggcttatct accgatgcgc
     8341 tggtcattga tatgtccacc atccatccgc tgcaaaccga taaattgatt gccgatatgc
     8401 aagccaaagg cttcagcatg atggatgttc cggtaggccg tacttctgca aatgccatta
     8461 ccggtactct gttactgctg gctggcggca ccgctgaaca agttgaacgt gccacaccga
     8521 tcctgatggc gatgggcagt gagttgatca acgctggcgg tccgggcatg gggatccgcg
     8581 ttaagctcat caacaactac atgagcatcg cgctcaatgc gctttcggca gaagccgccg
     8641 ttttgtgcga agccctgaat cttcccttcg atgttgccgt caaagtgatg agcggtaccg
     8701 ccgccggtaa aggtcacttc accacttcct ggccaaacaa agtcctcagc ggagatcttt
     8761 ctcccgcctt catgatcgat cttgcccata aggatcttgg catcgccctt gatgtcgcca
     8821 accagctgca tgtgccaatg ccgctggggg ccgcctcacg ggaggtttat agccaggcgc
     8881 gcgcagcggg tcgcggtcgc caggactggt ccgccattct ggaacaggtc cgtgtcagtg
     8941 ccgggatgac tgccaaagta aaaatgtaac gactggataa aggaataaat gaatgaataa
     9001 gtacaccatc aacgacatta cgcgcgcatc gggcggtttt gccatgctgg cggtcgatca
     9061 gcgcgaagcc atgcgcatga tgtttgccgc ggctggggca cccgctccgg tagccgatag
     9121 cgttttaact gatttcaaag ttaacgctgc aaaggccctc tcgccttatg cctcggcgat
     9181 tctggtagat caacaattct gctaccgcca ggtggttgag caaaacgcga ttgccaaaag
     9241 ttgcgccatg attgtcgccg ccgatgagtt cattcctggc aacggtattc cggtcgatag
     9301 cgtggttatt gaccgcaaaa tcaatccgct acagatcaaa caagacggtg gcaaagcctt
     9361 aaaactgctg gtgctgtggc gtagtgatga agatgcgcag caacgtctgg atatggtgaa
     9421 agagttcaac gaactgtgcc actcacacgg tctggtaagc atcattgagc cagtcgtccg
     9481 cccaccgcgt cgtggcgata aattcgatcg cgaacaagcg atcatcgatg ccgccaaaga
     9541 gctgggcgac agtggcgctg acctctacaa agttgaaatg ccgctttatg gcaaaggtcc
     9601 gcaacaagag cttctctgtg cttcacaacg tctgaatgac catatcaata tgccatgggt
     9661 gatcctctct tccggtgtcg acgaaaaact gttcccgcgt gccgtacgcg tggcaatgac
     9721 ggcgggcgca tcgggattcc tggcaggccg tgcagtctgg gcatcggtcg tcggtttacc
     9781 agacaacgag ctgatgctgc gtgacgtttg cgcaccgaaa ttacaacaac ttggcgatat
     9841 cgtcgacgaa atgatggcta aacgccgcta agatgaggat aaaaggaatg aaatggttta
     9901 acaccctaag ccacaaccgt tggctggaac aggaaaccga ccgcatcttt gattttggta
     9961 aaaattcagt agtgccgact ggttttggct ggttaggcaa taaagggcaa atcaaagaag
    10021 agatgggcac ccatctgtgg atcaccgctc gtatgttaca cgtttattcc gttgctgcgg
    10081 cgatgggtcg acctggcgct tactcgttgg ttgatcacgg tatcaaagcc atgaacggcg
    10141 cactgcgtga taaaaaatat ggcggctggt atgcctgcgt aaatgacgag ggcgtggtgg
    10201 atgcctccaa acagggctat caacacttct ttgctctgct gggtgccgcc agcgccgtca
    10261 caacgggtca cccggaagcg cgcaagctgc tcgattacac cattgaaatt atcgagaaat
    10321 atttctggag cgaagaagag cagatgtgcc tggaatcctg ggacgaagcc ttcagcaaaa
    10381 ccgaagagta ccgcggcggc aatgccaata tgcacgcggt ggaagctttc ttgattgttt
    10441 atgacgtcac tcacgacaaa aaatggctgg atcgcgcgat tcgcgtggct tccgtgatta
    10501 tccacgacgt cgccagaaat aatcattatc gcgttaacga acacttcgat acccagtgga
    10561 atccgctgcc ggattacaac aaagataacc cggcgcatcg cttccgcgcg ttcggaggta
    10621 caccaggcca ctggatcgaa tggggccgtt taatgctgca catccatgct gccctggaag
    10681 cccgttgcga acaaccacca gcatggctgc tagaagatgc caaaggtctg tttaacgcca
    10741 ccgtgcgcga tgcctgggca cccgatggcg cggacgggat tgtttatacc gttgactggg
    10801 aaggaaaacc ggtggtccgc gaacgtgtac gttggcctat cgtcgaagca atgggtacgg
    10861 cctacgcgct ctacaccgtc actggcgatc gccagtatga aacctggtat caaacatggt
    10921 gggagtactg cattaagtac ctgatggact atgaaaatgg ttcctggtgg caggagctgg
    10981 atgcggacaa taaggtcacc accaaagtct gggacggcaa acaggatatt tatcacctgc
    11041 tgcattgcct ggtgatcccg cgtatcccgt tagcgcccgg catggctcca gcggttgcag
    11101 cgggtctgct ggatattaat gcgaaataac tgatctgaaa atagggtgcg ggttttcccc
    11161 tcaccctaac cctctcccca aaggggcgag gggaccgtcc ggtgcagttt ttgggtttga
    11221 catcagcctc acgggaggct gtatgtcgtt aattaaggtg ccttgtatgc aaatcaccaa
    11281 tatgcattgc agtggacaga ccgtttcact tgccgcgggg gattatcacg cgaccattgt
    11341 gaccgtcggt gctggtctgg ctgaattaac ctttcagggt tgtcatttag tcatcccgca
    11401 caaacccgaa gagatgccgc tggcgcatct gggtaaagtg cttattccgt ggccgaaccg
    11461 gatcgccaac ggctgttacc gctatcaggg gcaggaatat cagttaccca ttaatgaaca
    11521 tagttcaaaa gccgctattc acgggctgct tgcctggcga gactggcaaa ttagtgaatt
    11581 aaccgccacc agcgttaccc tgacagcttt tctaccgccc agttacggct atccgtttat
    11641 gctggcttca caagttgttt attcgctcaa cgcacacacg ggtctttccg ttgagatcgc
    11701 aagccagaat atcggcactg tcgcagcgcc ttatggcgtg ggtattcata cttatttaac
    11761 ctgcaactta acatctgttg atgaatatct gttccagctt ccggcaaacc aggtttatgc
    11821 cgttgatgaa cacgtaaatc cgacaacgct tcaccacgtt gatgagctgg atttaaattt
    11881 cacgcaggcg aaaacaatag ccgcgacaaa aatcgaccac acctttaaaa ccgctaatga
    11941 tctgtgggaa ataaccatta cccatccaca acaggcgctt tcggttagcc tttgttgcga
    12001 tcaaccctgg gtacaaattt atagcggtga aaaattacag cgtcagggac tggcagttga
    12061 acctatgagt tgcccgccta acgcctttaa ttcaggtatc gacttattat tactggaacc
    12121 aggaaaaccg caccgcctgt tttttaacat ttacggacag agaaaataac ttgtattgat
    12181 tagcagtcaa aatcattgaa gaacatttca ctcgctaaag gcaacattat gtatcttcaa
    12241 tttattaagt taaaaacagc tcagcttctg taaagttttt atatatcagt tttatgagaa
    12301 tcattttaca taagcctgta ataacaggaa ggcaacaatc tcgttctcaa ctctatggag
    12361 attagttatg gatacgccac gtccacagtt attagatttt caatttcatc agaataacga
    12421 cagttttacc ctacattttc aacaacgtct tattttaacc catagcaaag ataatccttg
    12481 tttatggatt ggctcaggta tagcggatat cgatatgttc cgcggtaatt tcagcattaa
    12541 agataaacta caggagaaaa ttgcgcttac cgacgccatc gtcagccagt caccggatgg
    12601 ttggttaatt catttcagcc gtggttctga cattagcgcc acgctgaata tctctgccga
    12661 cgatcagggc cgtttattgc tggaactaca aaacgacaac cttaaccaca accgtatctg
    12721 gctgcgcctt gccgctcaac cagaggacca tatctacggc tgcggcgaac agttttccta
    12781 cttcgatctg cgtggcaaac cgttcccgct atggaccagt gaacaaggcg ttggtcgcaa
    12841 caaacaaacc tatgtcacct ggcaggccga ctgcaaagaa aatgcgggcg gcgactatta
    12901 ctggactttc ttcccacagc ctacgtttgt cagcacgcag aagtattact gccatgttga
    12961 taacagttgc tatatgaact tcgactttag tgccccggaa taccatgaac tggcgctgtg
    13021 ggaagacaaa gcaacgctgc gttttgaatg tgctgacaca tacatttccc tgctggaaaa
    13081 attaaccgcc ctgctgggac gccagccaga actgcccgac tggatttatg acggagtaac
    13141 gctcggcatt cagggcggga cggaagtgtg ccagaagaaa ctggacacca tgcgtaacgc
    13201 gggcgtgaag gtcaacggca tctgggcgca ggactggtcc ggtattcgta tgacctcttt
    13261 tggcaaacgc gtgatgtgga actggaagtg gaacagcgaa aactacccgc aactggattc
    13321 acgcattaag cagtggaatc aggagggcgt gcagttcctg gcctatatca acccgtatgt
    13381 tgccagcgat aatgatctct gcgaagaagc ggcacaacac ggctatctgg caaaagatgc
    13441 ctctggcggt gactatctgg tggagtttgg cgagttttac ggcggcgttg tcgatctcac
    13501 taatccagaa gcctacgcct ggttcaagga agtgatcaaa aagaacatga ttgaactcgg
    13561 ctgcggcggc tggatggctg acttcggcga gtatctgccc accgacactt acctgcataa
    13621 cggcatcagc gccgaaatta tgcataacgc ctggcccgcg ctgtgggcga aatgtaacta
    13681 cgaagccctt gaagaaacgg gcaagctcgg cgagatcctt ttctttatgc gcgccggttc
    13741 taccggtagc cagaaatact ccaccatgat gtgggcgggc gaccagaacg tcgactggag
    13801 tctcgacgat ggcctggcgt cggttgtccc ggcggcgctg tcgctggcaa tgaccggaca
    13861 tggcctgcac cacagcgaca ttggcggtta caccaccctg tttgagatga agcgcagcaa
    13921 agagctgctg ctgcgctggt gcgatttcag cgccttcacg ccgatgatgc gcacccacga
    13981 aggtaaccgt cctggcgaca actggcagtt tgacggcgac gcagaaacta tcgcccattt
    14041 tgcccgtatg acctccgtct tcaccaccct gaaaccttac ctgaaagagg ctgtcgcgct
    14101 gaatgcgaag tccggcctgc cggttatgcg cccgctgttc ctgcattacg aagacgatgc
    14161 gcacacttac accctgaaat atcagtacct gttaggtcgc gacattctgg tcgctccggt
    14221 gcatgaagaa ggccgtagcg actggacgct ctatctgccg gaggataact gggtccacgc
    14281 ctggacgggt gaagcgttcc ggggcgggga agttaccgtt aatgcgccca tcggcaagcc
    14341 gccggtcttt tatcgcgccg atagcgaatg ggcggcactg ttcgcgtcgt taaaaagcat
    14401 ctaatcacat ccgcccgtgg ggaaactcat gggcaaaggg agaacatcga tgagtcacat
    14461 cacaacggaa gatccagcaa ctttacgcct gccctttaaa gagaaactct cttacggtat
    14521 tggcgacctg gcctctaaca tcctgctgga tatcggtacg ctttatcttt tgaagtttta
    14581 taccgacgtt ctggggctgc caggcaccta tggcggcatt atctttttga tttcaaaatt
    14641 ctttactgcg tttaccgata tgggtaccgg cattatgttg gattcccgac gcaagatcgg
    14701 tccaaaaggt aagttccgtc cttttattct gtatgcgtca ttcccggtca ccttactggc
    14761 gatcgccaac tttgtcggca caccgtttga tgtcaccggt aaaacggtga tggccactat
    14821 tctgtttatg ctttacggac tgtttttcag catgatgaac tgctcctacg gcgcaatggt
    14881 tcccgctatc accaaaaacc ccaacgaacg cgcctcactg gcggcatggc gtcagggcgg
    14941 cgctacgctg ggcctgctgc tgtgcacggt gggattcgtg ccagtgatga atcttatcga
    15001 gggtaatcag caacttggct atatcttcgc cgccacgctg ttttcactgt tcggcctgct
    15061 gtttatgtgg atctgctact caggcgtgaa agagcgttat gtcgaaaccc aaccaaccaa
    15121 tccggcgcaa aagcctggcc tgttgcaatc tttccgcgca attgccggta accgcccact
    15181 gttcattctg tgtattgcca acctctgcac cttaggggcg tttaacgtca agctcgccat
    15241 tcaggtctat tacacccagt acgtacttaa cgatcccatc ctgttgtcgt atatgggatt
    15301 tttcagcatg ggctgtattt tcatcggcgt gttcctgatg cctggcgcag tcagacgttt
    15361 tggtaagaag aaggtctata tcggcggcct gctgatttgg gtgctgggcg atctgctcaa
    15421 ctatttcttc ggcggcggct cggtcagctt tgtggcgttc tcctgcctgg cgttcttcgg
    15481 ctcagcgttt gttaacagcc tgaactgggc gctggtttcc gacaccgtcg agtacggcga
    15541 gtggcgcacc ggcgtgcgtt cggaaggcac ggtctacacc ggcttcacct tctttcgcaa
    15601 agtgtctcag gcgctggctg gtttcttccc cggctggatg ctgacgcaaa ttggctatgt
    15661 gccgaacgtc gcacaggctg accacaccat tgaagggtta cgccagttga tcttcatcta
    15721 cccaagcgca ctggcggtag tcaccattgt ggcgatgggt tgcttctaca gcctgaacga
    15781 gaagatgtat gtccgcattg tggaagagat agaagcccgt aaacgcacgg cgtaataata
    15841 ataaacgacg ccctgcgggg cgttataagg agtgattatg tctgaccata atccactgac
    15901 attaaaactg aatctgcggg aaaaaatcgc ctatggtatg ggcgacgtcg gttcgaattt
    15961 aatgctctgc atcggtactc tgtatctcct caaattttat accgatgaat tagggatgcc
    16021 tgcttactat ggcgggatta tctttctggt ggcgaagttt tttaccgcgt ttaccgatat
    16081 gctcaccgga tttttactcg actcgcggaa aaatattggg cctaaaggca aatttcgtcc
    16141 ctttatttta tatgcagcag ttccggcggc gttaattgca acgctccagt ttatcgccac
    16201 caccttttgt ttgccggtta aaacgacgat tgccaccgcg ctgtttatga tgtttggact
    16261 ttcatacagt ctgatgaact gctcgtatgg tgcgatgatc ccggcaatta ccaaaaaccc
    16321 gaatgagcgc gcgcagctcg cggcttaccg ccagggtggt gcaaccatag ggctattgat
    16381 ttgtaccgtg gcgtttattc cgttgcagtc gcttttttct gactcaaccg tcggttatgc
    16441 ctgtgcggca cttatgttct ccattggcgg ctttattttt atgatgctgt gctacagagg
    16501 cgtcaaagag cattatgtgg acacagcgcc aaccggacat aaagccagta ttctcaaatc
    16561 tttttgcgcg atatttcgta atccaccatt gctggtttta tgcattgcta acctgtgtac
    16621 tctggcggca tttaatatca aactggcgat tcaggtctat tacacccagt atgtgctgaa
    16681 tgatattaat ttattgtcgt ggatgggatt cttcagtatg ggatgcatcc tcgtcggcgt
    16741 attactggtg ccagtaactg taaaatgttt tggtaaaaaa caggtttatt tagctggcat
    16801 ggtgctatgg gcggtgggtg atatactgaa ttatttctgg ggaagtaact ctttcacttt
    16861 cgtcatgttc tcttgtgtcg ccttttttgg cacggccttt gtaaacagcc tgaactgggc
    16921 actggtacca gataccgtag attacggtga atggaaaacc ggtattcgtg ccgaaggttc
    16981 tgtttatacc ggttatacct tctttcgtaa aatttctgcc gcacttgctg gcttcttgcc
    17041 aggcattatg ctgacgcaaa ttggttatgt tcccaacatc gcgcaaagcg atgcgacatt
    17101 acagggtttg cgtcagctca tttttatctg gccttgtgca ctggcaatta ttgctgcatt
    17161 gacgatggga ttcttttaca cactcaatga aaaacggttt gcattgatta ttgaggaaat
    17221 caaccaacgc aaaaataaag agatggcgac agaagaaaaa acggcttccg taacgttata
    17281 aatgtcaata gccgctattt ccatcctggt gggtggcggc ctccctacgt ttaaaaaatg
    17341 gacttattat gaaaaagatt aatgcaataa ttttattatc ttctctgacc tctgcctcgg
    17401 tatttgctgg cgcatacgtc gaaaacaggg aggcatataa tcttgcctcc gatcagggcg
    17461 aggttatgct acgtgtgggt tataacttcg atatgggcgc gggtattatg ttaaccaata
    17521 cctacacctt tcagcgagaa gatgaactaa aacatggcta taatgaaatt gaaggttggt
    17581 atcctttatt caaaccaacc gataaattaa ccatccagcc cggtggctta attaatgata
    17641 agagtatcgg ttcgggtggt gcagtgtatc tggacgtcaa ctataaattt gtaccatggt
    17701 ttaatctgac agtacgaaat cgctataacc ataataacta tagttcaaca gatttgagcg
    17761 gggaactgga taataatgac acctatgaaa ttggcaccta ctggaatttt aaaatcaccg
    17821 ataaattttc ctatacattt gagccacatt atttcatacg cgtaaatgac tttaatagca
    17881 gcaatggaaa agatcatcac tgggaaatca ctaatacctt ccgttatcgc attaatgaac
    17941 actggctgcc ttatttcgaa ttacgttggt tagatcgtaa tgtcgaaccg taccaccgcg
    18001 agcaaaacca gattcgtatc gggacgaagt atttcttctg atgtgaaact gccggatgcg
    18061 gcgtaaacgc cttatccggc ctacggttga tacggttttc gttggccgga ttaagcacat
    18121 ccggccatta atcaagcttc agccgccagc atctctttct tacgttggct aattcgctta
    18181 accagcatta cggaaaccac cgcaccacag aatgcgaagc aggccataat gccaaacacg
    18241 attttgtagc caataatccc cggatttaaa tccagaatgt agccatacag actgaagcag
    18301 aacatcgccg gagcgtaacc aataaagcta cccaacgcca tcgccgcgcc tgttttattt
    18361 tcagcaattt ttgcttcgcc gataggtgca aaaaatacgg ctcgctgtgt aaagactatc
    18421 gcgccaaagc ccagcgtaca tgccatccct aaatagaccg gcatactttc gtgcggcagc
    18481 ataatcaaca gtacgagcgc agcggtactg atgataaagg tgtagcatag atatttactc
    18541 ggcgatttca ggatcttatc tgaaatcatg ccaccaatcg gtccaccaat cattttcaga
    18601 cagtattggt taatgatgcc gtaagccccc accagcgcaa cgggcaatag atagatgttt
    18661 ttcaggaatg gaatgaagaa tgtcaggcca cagtaaaccg cgtaaacgaa gaagacgtta
    18721 aaagcgataa gccagatggt tttgtctctc agcaccgagg tcatactggt gttttgcgat
    18781 gctccgtctt cttttttcac ctcaacggac ggtgcctctt ctttgtcatt caggacaaag
    18841 aaaataataa tgcctacggc aatcacaata agggaataga accagatgcc tgctttaaaa
    18901 cctaataagc cactgccaaa ccaggtaaat actgccaacg cagaaaatgc caccacggta
    18961 tcgacaatgc cacgccctgt ttcgaagaag ccaaacaacc gaccttgttg ttcgctattg
    19021 cccaatcgac ttaccgactt gagcaagacc ggccagttca tcatgtcgca agtaacgcca
    19081 aatagcgccc agacaaagag gatgccccaa taccccggca ttgtcgtcaa ataaacaccc
    19141 agtaatcctg tcgcaatgag tgagaatgac atggtgtatc tgcgcggtag tttatcggca
    19201 aaataaatag acagaaaaaa gcccactgtg gtgacaaatg agtttaccga catagcatta
    19261 ccaatttgac cattggtcaa atggaaatat tcctgcatcg ggatataaaa cgcatctttc
    19321 agcgacggta atttataaat tgtaccgcca cacaatgtta atagactaaa taacgcccat
    19381 ttctttttcg tgagcataac atcccctaag cgatttttaa aatcactgtt ttaaaggcaa
    19441 taagataccg accattcata tggaatggtt ttattaactg cgtatgagtt aaaacttatt
    19501 agtggttcag aagatggttt gcctcttgtt tgatttgttg tagtacgtta cgcacatgat
    19561 ttatctgatt aattgcatct tctttttctt tacgtaaccg cgcatccagt aaatgatttt
    19621 ctggcaatgg tgtttcactc atctggcgat aagggatcca cggattatca tcttcgtctt
    19681 caaagcggaa cgccggcgca tagtaatcaa cctcttcttc caggccatat gccgtcacct
    19741 gcggctcatc atcacccagg catataaggt gcgttaataa cgctttgaac ggcatatcac
    19801 cctgacctga aatacaggct ttatgaccca ggccacccgg ttctttaacg atcaaggcat
    19861 ctttgatatg gacctgggta atatgcggcg ccatcgtttt taaagcgtcg atgggatgct
    19921 catttgcatt gatcatgttc gcaaaatcaa acagtaagga aagagattcc atctcacttt
    19981 ctttgaccag cgacaccagc tcatggcttt ttaaatcttc atgctgttcg atcgtaaaag
    20041 tcaggccgct gtcctgatac gtttcccgta cataggcaat gtcgttagcg atgatcgata
    20101 atacgtcgcg caaattacct tcataacgtg gataaaaacg tacggacgat gccccagttt
    20161 tcaacgcaat ggcgacggct tcgtcaatag atgccttatc tgaagcgctg gtttcaatat
    20221 gaatatcaag gttcagacgg cgggctttat ccccaaaggc agagagttct ttatcgtcca
    20281 tattaccaag agaaaaacgc tcgccatcca gaacatggat tttgacgcca cgtagattat
    20341 tttcacttgc gatatcaagt aaatcagcag gcaacactct ttccagccgc atatttaaat
    20401 gaaaggcata agcatgtaaa taaagaggaa gagtgtcgac acgttgtaga atctttcttg
    20461 cgttatttat tgtcaccata aaacacctac agattaatga tgaggtctac ggcctcaaat
    20521 cgccaatatt caatatctat ctggacaata ttgccttgct ggtcggcgcg atgcttttca
    20581 attaagatcg ctggcatacc cgcagaaccg ccaagatact tgctgatatc gcccggcatt
    20641 ctgacgggtt tgaaaatcaa tttttttact accgtctctt tttggtagca cttttcccag
    20701 actgcagaaa atgagtggtt ttcaagttgt tcaataaaac ccggagcaac ttcaggatta
    20761 atatatgttt cgtgatagaa aactttatgt ccttccagcg ccccccagcc ggtgatgcga
    20821 tagagttcag ttgatggtgt cactgcaatc agtggcgcga gcgtcttggg aatatcgctg
    20881 gtacggtttt tctcggtaaa cccccaggaa ggctctcgtc cttgctcaat tgcggcccgc
    20941 tgaaagctcg ccgacaactc cggactgtaa ttaaatcgcg gctgggtcac gaaccacccc
    21001 ttacgatcct tacggaagat tttggattct gcctcgaggt tcaacaacgc ctggcgcagc
    21061 gtcatacgtt taatgcccag cagttctccc agctctcttt ctgaagggag ttttccaccc
    21121 ggcgtggtga tgccatcttt caaccaccga tccagtttct cttttgcatt ttctacggtg
    21181 gattgattct ccgccattgt ttaatccttt tggtttaaac cagatgaagc atcttacgct
    21241 tacgccaaaa aagaaagcaa ttaacggcgt atgactttgc agataattgt gacaaagctc
    21301 tctattgacg taatcccaga cttataaggg agatactcgc attggtgtgc gctcacgaca
    21361 ggtaaaaaaa aacctgccag cgatggcagg ttttttataa ctaaaaaacg aaattaatcg
    21421 tctttcggtg cgcggttggc gcggcgacga tcgttttccg tcaggtgacg tttacgaata
    21481 cggatagagg tcggagtcac ttctaccagt tcgtcatcat cgatgaactc cagagcttgt
    21541 tccagagtca tgcggatagg cggaaccaga acaacggctt cgtcagtacc ggaagcacgc
    21601 atgttggtca gtttcttacc ggtcaggcag tttaccgtca ggtcgttaga gcggctatga
    21661 ataccgataa tctgaccttc gtaaacttct gcaccgtgac cgaggaacag cttaccgcga
    21721 tcctgcagac cgaacagcgc gaacgcgacc gctttaccct gaccgttaga gatcagtacg
    21781 ccgttctgac gctgacccac ttcacccgga cgtacgtcgt cgtagtggct gaaggtggag
    21841 tacagcagac cagtaccgga agtcatggtc atgaactcag aacggaagcc aatcagacca
    21901 cggcttggga tcacgtagtc gagacgtacg cggcctttac cgtctggatt catgtttttc
    21961 aggtcgcctt tacgttcgcc cagcgcctgc attacagaac cctgatgctg ttcttcaacg
    22021 tccagagtca cgttttcata cggctcttgt ttacgaccgt cgatttcacg gaagataact
    22081 ttcggacggg ataccgccag ttcgaaacct tcacgacgca tgttttcgat cagaacagac
    22141 aggtgcagtt cgccacgacc agaaacgcgg aacgcatcgg cgtcttcggt ttcttctacg
    22201 cgcagcgcaa cgttgtgtac cagttctttg ttcagacgat ccaggatctg acgagacgtt
    22261 acgaacttac cttctttacc gcagaacggc gaggtgttaa cgcagaagaa catagaaacg
    22321 gtcggctcat caacggagag tgccggcagc gcttcaacgt tttgcgtgtc gcaaacggtg
    22381 tcagaaatgt tcagttcgcc aaggcccgtg atcgcaacga tatcgccagc ttccgccaga
    22441 tcggtttcga tacgttccag accgaggtgg cccagcactt taccgacttt cgcgttgcgg
    22501 gttttgcctt cgctatcgat gatagtgacc tgctggttcg gcttcacttt accgcgcttg
    22561 atgcggccaa tgccgataac gccaacatag ctgttgtaat cgagctgaga aatctgcatc
    22621 tggaacggac cgtcaaggtc aacgtccggc gcaggaacgt ggtcaacaat cgcctggtac
    22681 agcggggtca tgtcttccgc catatcttcg tggtccagac ccgcgatacc gttcagcgca
    22741 gaagcgtaaa cgatcgggaa gtccagctgc tcgtcggtcg cgtcgaggtt aacgaacaga
    22801 tcgaatacct gatccacaac ccaatcagga cgcgcgccag ggcggtcaac tttgttgata
    22861 acaacaatcg gcttcaggcc gtaagcaaac gcttttttgg ttacgaagcg cgtttgcggc
    22921 atcgggccgt caaatgcgtc aaccaccagc agcactgagt ctaccatgga cattacacgt
    22981 tcaacttcac caccgaagtc ggcgtgcccc ggggtatcaa cgatgttgat acggtaatca
    23041 ttccatttga tagcggtgtt tttcgcgagg atggtaatcc cccgctcttt ctccaaatcg
    23101 ttggagtcca tcacgcgctc ttgggtttcg gcacgagagt cgaacgtacc ggattgttgg
    23161 agcagcttgt ctaccagggt ggttttacca tggtctacgt gcgcgatgat ggcgatatta
    23221 cgcaattttt cgatcacaac tttgcctcag gcattagaaa tagcgcgtta ttgtacacgg
    23281 attaatcgca ctacaaaaca ggatcacaaa catcctccgc aaacaagtat tgcagagtcc
    23341 ctttgtgatc gctttcacgg agcataaaaa gggttatcca aaggtcattg caccaacatg
    23401 gtgcttaatg tttccattga agcactatat tggtgcaaca ttcacatcgt ggtgcagccc
    23461 ttttgcacga tggtgcgcat gataacgcct tttaggggca atttaaaagt tggcacagat
    23521 ttcgctttat cttttttacg gcgacacggc caaaataatt gcagatttcg ttaccacgac
    23581 gaccatgacc aatccaggag agttaaagta tgtccgctga acacgtactg acgatgctga
    23641 acgagcacga agtgaagttt gttgatttgc gcttcaccga tactaaaggt aaagaacagc
    23701 acgtcactat ccctgctcat caggtgaatg ctgaattctt cgaagaaggc aaaatgtttg
    23761 acggctcctc gattggcggc tggaaaggca ttaacgagtc cgacatggtg ctgatgccag
    23821 acgcatccac cgcagtgatt gacccgttct tcgccgactc caccctgatt atccgttgcg
    23881 acatccttga acctggcacc ctgcaaggct atgaccgtga cccgcgctcc attgcgaagc
    23941 gcgccgaaga ttacctgcgt tccactggca ttgccgacac cgtactgttc gggccagaac
    24001 ctgaattctt cctgttcgat gacatccgtt tcggatcatc tatctccggt tcccacgttg
    24061 ctatcgacga tatcgaaggc gcatggaact cctccaccca atacgaaggt ggtaacaaag
    24121 gtcaccgtcc ggcagtgaaa ggcggttact tcccggttcc accggtagac tcggctcagg
    24181 atattcgttc tgaaatgtgt ctggtgatgg aacagatggg tctggtggtt gaagcccatc
    24241 accacgaagt agcgactgct ggtcagaacg aagtggctac ccgcttcaat accatgacca
    24301 aaaaagctga cgaaattcag atctacaaat atgttgtgca caacgtagcg caccgcttcg
    24361 gtaaaaccgc gacctttatg ccaaaaccga tgttcggtga taacggctcc ggtatgcact
    24421 gccacatgtc tctgtctaaa aacggcgtta acctgttcgc aggcgacaaa tacgcaggtc
    24481 tgtctgagca ggcgctgtac tacattggcg gcgtaatcaa acacgctaaa gcgattaacg
    24541 ccctggcaaa cccgaccacc aactcttata agcgtctggt cccgggctat gaagcaccgg
    24601 taatgctggc ttactctgcg cgtaaccgtt ctgcgtctat ccgtattccg gtggtttctt
    24661 ctccgaaagc acgtcgtatc gaagtacgtt tcccggatcc ggcagctaac ccgtacctgt
    24721 gctttgctgc cctgctgatg gccggtcttg atggtatcaa gaacaagatc catccgggcg
    24781 aagccatgga caaaaacctg tatgacctgc cgccagaaga agcgaaagag atcccacagg
    24841 ttgcaggctc tctggaagaa gcactgaacg aactggatct ggaccgcgag ttcctgaaag
    24901 ccggtggcgt gttcactgac gaagcaattg atgcgtacat cgctctgcgt cgcgaagaag
    24961 atgaccgcgt gcgtatgact ccgcatccgg tagagtttga gctgtactac agcgtctaat
    25021 agttgaagtt gtactacccg gcgcaacaac gccggaggtt caaaccaggc ccatctggcg
    25081 taattgttgc agccagtttg aacacggaca gcgcgcagaa cccggagcgt acactggtac
    25141 gtgaggagtt cgagcactgc ccaggttcaa aatggcaaat aaaatagcct gatgggactg
    25201 gtttttagtt gccgtggaaa tttcagccca tctctgcatg ggcttttttc tccgtcaatt
    25261 ctctgatgct tcgcgctttt tatccgtaaa aagctataat gcactaaaat ggtgcaacct
    25321 gttcaggaga ctgctttatg gcaacaggca cgcagcccga tgctgggcag atcctcaact
    25381 cgctgattaa cagtattttg ttaatcgatg acaacctggc gatccattac gccaaccctg
    25441 ccgcgcaaca actgctcgcc caaagctccc gcaaattgtt tggtacaccg ttaccggaac
    25501 tgttgagcta cttctcatta aatatcgagc tgatgcaaga aagtctggag gcggggcaag
    25561 gttttaccga taacgaagtg acgctggtca tcgacgggcg ctcgcatatc ctttctgtga
    25621 cggcccagcg tatgccggac ggcatgatcc tgctggagat ggctccgatg gataaccagc
    25681 gccgcttaag tcaggaacag ctacagcacg cccagcaggt tgctgcccgt gatttagtgc
    25741 gcggcctggc acatgagatt aaaaatccgc ttggcggttt acgtggcgcg gcgcagctgc
    25801 tcagcaaagc gttacctgac ccatcactac tcgaatatac caaagtgatt atcgaacagg
    25861 cggaccggct gcgaaatctg gtcgaccgtc tgttggggcc gcagctgccc ggtacgcgcg
    25921 ttaccgaaag tattcacaaa gtggctgaac gcgtggtaac gctggtgtcg atggaactgc
    25981 cggacaacgt gcggttgatt cgtgattacg atcccagcct gccggaactg gcgcacgacc
    26041 cggatcaaat tgaacaggtc ttgctgaata ttgtgcgcaa tgcgctacag gcgctggggc
    26101 cggaaggcgg tgaaatcatt ctgcgtaccc gcaccgcgtt tcaactgacc ttacacggcg
    26161 agcgctaccg gctggcggcg cggattgatg tggaagataa cgggccgggc attccgcctc
    26221 atttgcagga tacgctgttt tacccgatgg tcagcggccg cgaaggtggc accgggcttg
    26281 gcttatccat cgctcgtaat ttgattgatc agcattcagg caaaattgaa tttaccagtt
    26341 ggccagggca taccgagttc tcggtttacc tgcctatcag gaaataaagg tgacgtttat
    26401 gcaacgaggg atagtctggg tagtcgatga cgatagttcc atccgttggg tgcttgaacg
    26461 tgcgctcgct ggggcaggtt taacctgtac gacgtttgag aacggcgcag aagtgctgga
    26521 ggcgctggcg agcaaaacgc cggatgtgct gctttcagat atccgtatgc cgggaatgga
    26581 cgggctggcg ctgctcaagc agattaaaca gcgccatcca atgcttccgg tcatcattat
    26641 gaccgcacat tccgatctgg atgctgccgt cagcgcctat caacaagggg cgtttgatta
    26701 tctgcccaaa ccgtttgata tcgacgaagc agtggcgctg gttgagcgcg ctatcagtca
    26761 ttaccaggaa cagcagcagc cgcgtaatgt tcagcttaac ggcccaacga ccgatatcat
    26821 cggcgaagcg ccagccatgc aggacgtgtt ccgtattatc ggtcggcttt cgcgttcttc
    26881 tattagcgtg ctgattaacg gcgaatccgg caccggtaaa gaactggtcg ctcatgccct
    26941 gcatcgccac agtccgcgcg ccaaagcgcc gtttatcgcg ctgaatatgg cagctatccc
    27001 aaaagatttg atcgaatcag aactgtttgg ccacgagaaa ggcgcgttta ctggcgcgaa
    27061 taccattcgt caggggcgtt ttgaacaggc cgatggcggt acattattcc tcgacgaaat
    27121 tggtgatatg ccgctggatg tgcagacgcg tttgctgcgc gtgctggcag acggtcagtt
    27181 ttaccgcgtt ggcggctatg cgccggtgaa agtggatgtg cggattatcg ctgccactca
    27241 ccagaatctc gaacagcgag tgcaggaagg taagttccgt gaggatctgt tccaccgcct
    27301 gaacgttatc cgcgttcatc tgccgccgct gcgcgaacgt cgggaagata ttccccgtct
    27361 ggcgcgccat tttttacagg ttgccgcgcg cgaactgggc gtagaagcga agttactgca
    27421 tccggaaacc gaagctgctc tgacgcgtct ggcgtggcca ggcaacgtgc gccagctgga
    27481 aaacacctgc cgctggctaa cggtgatggc cgccgggcag gaagtgttga ttcaggattt
    27541 gcccggcgaa ctgtttgaat caacggttgc ggagagtact tcgcaaatgc aaccggacag
    27601 ctgggcgacg cttcttgcgc agtgggcaga cagagcgctg cgttccggtc atcaaaatct
    27661 gctttccgaa gcgcagccag agctggagcg gacgttactg acgaccgcgt tgcgacatac
    27721 gcaggggcat aaacaggaag cggcgcggct actcggctgg ggccgcaaca ccctgacgcg
    27781 taagttaaaa gagctgggga tggagtgatt cacagcttgt gtgtaaagat tgattattga
    27841 gcgcaaattg ctggtatttt acgctttact gttccgataa gttcagtatg atcttgcccg
    27901 gaaaacgggg agagtcatta tgctggaatc aataattaat ctggtatcga gtggcgcagt
    27961 tgacagccac acaccgcaaa ctgccgttgc tgccgtgctg tgtgccgcaa tgattgggct
    28021 gtttagttga gtttcagatt gctgatgaag tgctctttgt tcatgccgga tgcggcgtga
    28081 acgccttatc cggcctacaa aagcaatatt ttcaacaggt tttgtaggcc tgataagcgt
    28141 agcgcatcag gcaatcttgt tctcagcaca ttgatggcgc tttcgttttc tactttgtaa
    28201 acgaagcgcc attcactatt taaatcaccc gtgagaactg ctgcatccgc gctttctggc
    28261 gcagataggt atcaaagcac atgcaaatgt tgcggatcag caagcgacct ttcgccgtca
    28321 cctgtattcc cttctcatcc acatccacca gcccatcttt tgctaacggg gcgagcagct
    28381 tgagatcttc cgcaaagtaa tcagcgaagt gcaaatccca cagtttctca ataggggcgt
    28441 aatccagacg gaagttgcag atgagcgact taatcacatc gcggcgaata cagtcatcac
    28501 gcgttagcgc aataccacgc cacagcgcat tgccttgttc atccacttgc tgatagtact
    28561 gcttcaactc tttctggttc tgcgcgtagc agtcgccaat catgctgatg gcggaaacgc
    28621 ccatccccag cagatcggta tcgccctgag tggtgtagcc ctggaagtta cgatgcagca
    28681 cgccttcacg ctgggccacc gccagctcgt catccggacg ggcaaagtga tccataccga
    28741 taaactgata gcccgattgc gtcaggaagg cgatggtttc ctgcaggata tcgagttttt
    28801 gctgcggact cggcaggtca gcatctttga ttttgcgctg agcagcaaaa atggtcggca
    28861 gatgcgcgta gttaaagaca ctcagacgat cggggttcag ctccgccaca cgtttcaggg
    28921 taaaggcgaa actctccggc gtctgtttcg gcaggccgta aatcaggtcg atgttggtgg
    28981 aggtaaaacc aatctcacgc gcatggttaa gcagtgcaaa gatgaactct tcatcctgct
    29041 cgcggttaac cagacgttgc acttctttgt tgaagtcctg cacgcccatg ctcaggcgat
    29101 taaagccttc ggcgcgtaaa tgatcgagta catccagttc gatttcccgc ggatcgactt
    29161 cgatcgaaat ctccgcatcg gcattgaact ggaagttttc gcgcagcagc ttcatcaggc
    29221 ggctgatttg cgctttattc agatacgtcg gcgttccgcc gccccagtgc aattggctga
    29281 cgtgacgccc ggcgaacagc ggtgcacgat ggacgatttc ttgctccagc gcgtccagat
    29341 actgatcggc cttgtgctgc tggcgagtaa caatcttatt gcaaccgcag aagtaacaaa
    29401 gcttatggca gaacgggata tgtacgtaga gagataatgg acgctcagga tagcgcgcca
    29461 cggcttgtaa aaacgcctgt tcgccgaagt cttctgaaaa ctccagcgcg gtcgggtacg
    29521 aggtgtatcg tggcccggaa tagttatatt tctggatcag ggccagatcc cagtcgattt
    29581 gctgtacaga catgctcact ccttccgatg gcgtctctgg cggctacggc gtaccggcgg
    29641 atgcccgttt cgggtcatca gccggttgcg cagccacttt tgtcgccgcg acaaccgctg
    29701 tagtttaacg aataaccaca ccagataaca tataaccgga agggttatca gcagaacggg
    29761 aaggcccacg gcggcaatcc gttagttgcc ccgcagcaga cgcatcatgt cttcttgctt
    29821 ctcgtcttct tcctcttctt cgtcatcgtc ataagagagg ccgagtttct gcatcaactc
    29881 atcaatacga tccagtttag catccaccca ggattgctct tcggcgctca gggtttcgcc
    29941 tgcttccaga cgttccagca gcgcatccag acgctcatcc gtttccagta attccaactc
    30001 cgcctgcggt gaaagcatag gtttctcact cttcggtttg tgctgtttgg tgactttttc
    30061 agtcacgccc aatggaatag gggttttact gccaatacgt ggatcttttg gtgcgttctg
    30121 gcctttgctg cctgacgtgg tgtttccgcc cgctgcgcgg ctgcccggcg catgaccacg
    30181 acgttttttc tgacgcttgc gatcacgagc ttcctgatcc agctcctcgc gtgtttttcg
    30241 acgcgctttt gcatgacctt tgctgcgtga gtttgaagat gatggtttca taatggttcg
    30301 tcttttagcc gttttattca gtatagattt gcggcggaat ctaacagaaa gcaagcaaag
    30361 aaaaaaggcg acagattact ctgtcgcctt ttttcctgac tcataaccct taacgggtct
    30421 tacaatcctt gcaggccgcc aacacaccct gtttttcctt tagccggaac aatccgtgtt
    30481 gattccattt ccgtttaatt acgtctccgg acgtttgtct tcctgacaat cctctgtctt
    30541 cgcctcctgg cgctcctgac cctcatcctg agtcattgtt cctgttagcg tcctcgctct
    30601 atgggtgcta ctttacgcct ttgctttaaa caaacaatca accgccatca gtatttgcac
    30661 attttcagag ttttatataa aagcaattca ctgatttata tagcttattt gacatgcatc
    30721 gcagaatttt ctctgctatt tcgcataaag actatcggca atgtctcaca aagcgtatgg
    30781 cttttactta caacgctcac gccgaaaaat ccgcctttta ttatggttca ggtataatcg
    30841 ccacaaagct ttgactgacg gagacgaccg ctttgactaa tttgaattat caacagacgc
    30901 attttgtgat gagtgcgcct gatattcgcc acctaccttc cgataccgga attgaagtgg
    30961 cttttgcagg ccgttccaac gcaggtaaat ccagcgcgct gaacacgctg actaaccaga
    31021 aaagcctggc tcgtacctca aaaaccccag ggcgcaccca gcttatcaac ctgtttgaag
    31081 tggctgacgg caagcgtctg gttgacttgc ctgggtacgg ttatgcggaa gtcccggaag
    31141 agatgaagcg caaatggcag cgtgcgctcg gcgaatacct cgaaaaacgt cagagcctgc
    31201 aaggtctggt ggtgctaatg gatattcgcc atccgctgaa agatttggat cagcagatga
    31261 ttgagtgggc ggtagacagc aatatcgccg ttctggtgct gctgaccaaa gcggacaaac
    31321 tggcaagcgg cgcacgtaaa gcgcaattga atatggtgcg tgaagctgta ctggcgttta
    31381 acggtgatgt gcaggttgaa acgttttctt cgttgaagaa acaaggcgtg gacaagctgc
    31441 ggcagaaact ggatacctgg tttagcgaga tgcagcctgt agaagaaacg caggacggcg
    31501 aataattttc ttgccttaat gcttgtgccg gatgtggcgt atccggcccg taaattcaat
    31561 agattacaat tctaccgttg gcctgataaa catagcgcat caggcattac ggatcttttc
    31621 tttcgcccaa taaaaaacgc cccagtcatt actgactggg gcggctaaaa tattcagcca
    31681 aatccgatta cgtgaagtaa aaggtctgaa agatagaaca tcttacctct gtaccctacg
    31741 cgactaactt tactcttttt tgttcagttc agaaagcact ttttgtaatt ttttttcagt
    31801 tgttgcatag gaatttctaa tagccatcac aaaacgtgac agcttatgtt gcttacttac
    31861 gaaaaaaggg catgttcagg cgaatcttag tgcgcctgat cccagttttc gccactcccc
    31921 acttccacca gcaacggcac atccagacgg gtacagtttt ccatcagttg atgaatctgc
    31981 ttcgcgacgg catcaacatc atctttatga acttcaaata ccagttcatc gtgtacctgc
    32041 atgatcatac gtacacgcgg ttgctcagcc tgtaaccacg catcaacggc aatcatcgcc
    32101 cgtttgataa tgtcggcggc ggttccctgc attggcgcgt taatggctgc acgttcagcc
    32161 gctgcacgac gagcaccatt gctggatttg atatccggca gatacagacg gcgtccgtcc
    32221 agcgtttcaa cgtagccctg ctctttcgcc tgagcacggg tgcgttccat atactccagc
    32281 acgccagggt agcgttcgaa gtaaaggtcc atgtacttct gcgcttcttt acgtggaatg
    32341 ttcaattgcc gcgccagacc gaaagcactc atgccataaa tcagaccaaa gttgatcgct
    32401 ttcgcgctac ggcgttgctc gctggtgacg gtttccagtg gcaaactaaa cacttctgcc
    32461 gccgttgccc ggtggatatc ttttccttcc gcgaatgcgg tcagcaagcc tttgtcacgc
    32521 gaaagatgcg ccataatgcg cagttcaatc tgcgagtagt ccgctgagac aatcacataa
    32581 tcctctggcg caataaacgc ctggcggata cgacgacctt cttcgttacg caccggaatg
    32641 ttttgcaggt taggatcggt tgacgataaa cgtcccgttg cagttactgc ctggtgataa
    32701 gaggtatgca cacgcccggt tttcgggttg atcatcagcg gcagcttgtc ggtgtaggtc
    32761 gatttcagct tcgccagacc acgatactcc agaatcactt ttggcaacgg atagtccagc
    32821 gccagttctt ccagtacctc ttccgacgtt gacggcgcgc cacccggcgt tttcttcagc
    32881 ggtttaatac cctgtttttc aaagagaatg gtttgcaact gcttggtgga agaaaggtta
    32941 aattcttcac ctgcaatttc atgcgctttc ttttccagct cagccagacg caaggtgagc
    33001 tcttcagaat gattgtgcag cactttcgga tcgatcttca caccgttacg ttcaatgcgt
    33061 gaaagcaccg gcaccagcgg catttcgata ttctcgaaga cgttcaacgg ccctttgtgt
    33121 ttttgcagat ctggccacat tttcagatgc aactgcaagg tgacatcagc atcttcggcg
    33181 gcgtaacgtc cggcctcttc gagggcaatc tggttaaagg tcagttgatt tttgccttta
    33241 cccgcaatct cttcaaaagt gatggttttg tgcttcaacc aacgttccgc gaggctgtcc
    33301 atatcgtgac gcccggcaac gctattgaga atgtaggact ccagcatggt atcaaacgca
    33361 atcccacgca gctcaatgcc gtaattcgcc agaataccgc gatcgtattt caggttttgc
    33421 ccgaccttca gcgccttttc atcttccagc agcggtttta gcaactcgag tgcacgctcg
    33481 cgagagattt gatcgggcgc gtcaagataa tcatgagcaa ccggaatata tgccgctacg
    33541 cctggctcga tagcaaaaga aagcccgacc aggttagcag agatgttatc aaggctgtcg
    33601 gtttcggtat caaatgcaaa taccggcgct ttttccagct tcgcaatcca cgctttcagt
    33661 gtttcttcat caaggatggt gacgtagttg tcataagaaa tcaccgttgc cgtcacttct
    33721 ggcgcttcgt ctgcaacact ggtttcctgc ggcttcgcgg ctggttttgc ccctttggcc
    33781 tgtaaccatt tgcccgcttc gacatcagca gtccagcgtt tgaactcata ctttttgaac
    33841 agccccaaca actcttccgc tgccggttgc tgcacttcca gttgttcaca ggtcagctcc
    33901 agttcaacgt cggttttaat cgtcgccagc tggtatgaga gataagcaac ttctttgttt
    33961 tgctcgagct tcgctgccat tgttttcgcg ccacggaagc tcaacccagc aattttttct
    34021 ggctcggcat acagcgtatc cagcccgcca agaccttgca gcaatgcctg cgcggttttt
    34081 tcaccgacgc ccggtacgcc aggaatgtta tcggaggagt cacccatcag cgccaggaaa
    34141 tcgatgatca gttctggcgg cacgccgtac ttattcacca cctcttccgg tccgaggatg
    34201 gtattcgtca tggtattgat aagcgtaata tttggcgtca ccagctgcgc catatcttta
    34261 tcgccagtgc tgatcagcac cggacgcccg gctttttcgg cttcgcgcgc cagagtaccg
    34321 ataacgtcgt ccgcttctac gccagaaacc gccagcagcg gcagtcccat cgctttaacc
    34381 atcgcgtgca ggggttcgat ttgtgctcgc agatcgttcg gcattggcgg gcgatgtgat
    34441 ttgtaatgtt caaacagttc atcacgaaag gtttttccct tggcgtcaaa gaccaccgct
    34501 gcatgcgtcg gtttatattg catgatcaga ctgcgcagca tgttgaggac accatacatc
    34561 gcaccggtcg gctcgcctgc gctgttagtc agcgggggaa acgcgtgata tgcgcgataa
    34621 agataagatg aaccatctac aaggataagt ggattttggg ggatctgaac cataatgtcc
    34681 gtgcctgttt atcagattat gggtaaagga tgccacagac aggatgaaaa catgagtttt
    34741 tcaccgcttt tcgtgaaaat cttttgtaga tcttctggat cgctcgcaaa tcgtcatgtg
    34801 gataactttg tgcataaatt atttacatcg cttttaattt attatcatgc tttttacagc
    34861 atgatatttg tctttgtttt tcatgcagtt aaaaataaag ccatccctaa taattgagat
    34921 aatgggattt tttctaatgt ggatagatat gaattatttt tctccttaag gatcatccgt
    34981 tatttgggtc gttttctgct aaaatctgcg ccttgcgctt ctttagcgca ctgtctatgg
    35041 ctaacctttt gaataaattc attatgacga gaatactcgc tgcgataacc cttttgctga
    35101 gtatcgtatt gaccattttg gtcactattt tttgttccgt cccgatcatc attgccggga
    35161 ttgtaaaact attgctacct gtgccagtca tttggcgaaa ggtttcgcgt ttttgtgatt
    35221 ttatgatgta ttgctggtgt gaaggtctgg cggtattact gcatctgaac ccacacctgc
    35281 aatgggaagt tcacgggctg gaagggttaa gtaagaagaa ctggtatctg cttatttgta
    35341 accaccgtag ttgggcagat attgtcgtac tgtgcgtgct gtttcgtaag cacattccga
    35401 tgaataaata tttcctcaag caacagctgg cctgggtgcc atttcttggc ctggcgtgct
    35461 gggcgctgga tatgccgttt atgaagcgtt attcccgcgc ttatttatta cgtcatccgg
    35521 agcgacgtgg taaagatgtt gaaaccactc ggcgttcttg tgaaaagttt cgcctgcatc
    35581 ccaccaccat tgtcaatttc gttgaaggct cccgtttcac gcaagaaaaa catcagcaaa
    35641 cccactccac ttttcaaaac ttgttgccac caaaggcggc aggcattgcg atggcgctaa
    35701 atgtactggg taaacaattc gataaactgc ttaatgttac gctgtgttat ccggacaata
    35761 accgtcagcc gttcttcgat atgttaagcg gtaaactgac gcgaattgtc gttcatgtgg
    35821 atttacagcc tattgccgat gagttacatg gcgactacat caatgacaaa agctttaagc
    35881 gccattttca gcaatggctg aactcactgt ggcaggaaaa agaccaactt ctgacgtcgt
    35941 tgatgtcatc acagcggcag gacaagtaaa ttttgctcgc ctctgaatga gcagaggcga
    36001 gtgagtattt taatgaacaa aacgtccggc acgagacata aattcttctt cgctcatctc
    36061 ctgtccgtta aaaacaactt tacccggtgt ataacgcagt tgcagcgagg cggtattgtt
    36121 gtcaatcgtg attaactgaa acatctgacc taaggtcatc atcccgctga tttgtttatc
    36181 agcccgcttt tgcgcttttt ccgcatccat tccttcagat aaattaagct gttttgcggt
    36241 ttctgtgacg acattaagcg gtaacttcac atcaaaattg agcgatttaa tatctttgtt
    36301 tgtggatgat gaagatttgg ctgggtcggc aatactgata tccagattgg catttagttc
    36361 gccgagtgcg ttcttccatt ttaccggttg tttaatggtc ggctcacttt tttgtaataa
    36421 cggtaaatac tctttgaaca atgcagcatt cacttcttgc agagcaactt catcgtttgc
    36481 taactcaggg tgtgcagcaa gctgcttctg cattgcaatg ttatactgga tgataaattg
    36541 gcgcaccgct gagggatcaa tagattcagc aatcaatgac cactcaccac taccgagttg
    36601 ctgattccct tttgttaact tatcaagttc gtaggtcaga ttgacattga tataatcttt
    36661 gtctttaacg cgatccagca tggtttttgc aacgaaagca tcgatttggg caacgtcttc
    36721 cccgtgattg ataatgttaa tctgattaaa tttcgactcg ctttcaccaa ccggaaattt
    36781 cttttcttct aatcgcgcca gagaatcaat ggtaaaactt tttgccgtta ctttgttcat
    36841 tggtgataat tgcagaacaa tatcttcgac tttgccttca atatccaggt tagacaatga
    36901 actaccatca ccattaaaag taaactggcc ctcaccaaaa gaaaactttc catactctgc
    36961 aggggcaaca tctaatgtcg tagagaatga gccaccaaac gcgaatcgca atgtcgcttc
    37021 cacgggcgat ttatttttcg cggcgataaa tagcggttgt gtcagttcat tattcactaa
    37081 gttcactttt gccgctgcca gtgctgggat gacattacca tgcattaaca ttgtgatggg
    37141 caacggaccg tgctcaacat ccacatcaaa tacaactttc tggcctggct tgatattgag
    37201 atcgggtgca ccgttatcga atgtcatttg catttgaaaa cgggaattaa aaaagccttt
    37261 gtcaaagttt ttataactta acgtcatatc gtaggcatgt tcacctttct ttttcgcatc
    37321 gttaaaatct ttaataaact tttcgatacc aggctgaatt tgcgtacctg tgtaccatgt
    37381 gccaccaccc cagattacgg ctaacgcaac aataacacct gtagctgact tacgtatcat
    37441 ataaatattc cctgataaaa cagcaaaact ttgaatatcc acttatgctg aaataatgtc
    37501 cagcggcagg atgcattatc agaatgtatt tatatttatt aaataataaa aaaagcccgt
    37561 gaatattcac gggccttatg taatttacat tgaattattt tttctcggac agatatttca
    37621 ctgtatcagc atactgctga acaaaaacat ccatattgct ggtatccata ccctgcggat
    37681 tcagctgata tttaccgtta acaaacatcg ccggaacgcc acgcaattgc acgtcagctg
    37741 cagctttttc ctgctgagcg accagagatt tgaccacgaa gctgttccac gccgcgtcgt
    37801 actcttcacc tttaatacct gcgttgataa atacatcgcg gatatcagat gctgaacgaa
    37861 tggtctgggt tttctgtacg ccttcaaaca gcggaacagt gactttgtct tccacgccca
    37921 gcgccatcgc cacagcccat gcctgagtca gatctttgcc caggtcacca cccatgaagt
    37981 tgacgtggta tttagtcatc ttcacgcctt ccggcagttt tttcttcaca ttatcagaaa
    38041 tatgcagaac ttcttcaaac tgatagcagt gcggacagaa gaaagagaaa aactccagca
    38101 cttgcggcgc gccagctacc ggtttttcca gggtagtgta ctgtttacca tcttcatact
    38161 gcgccgccga tgcgctaaac gctaaaacta aaccagccag cgccagccaa atctttttca
    38221 tgatctactc tctccgatta atacataggt gttaattgta aagggggttc ttgtagaact
    38281 tttgcctgtt ctataaaagt cgccgtttgt cgcagccagt aatcttcccc ggttaaccac
    38341 gggaaatttt tcgggaacgc gggatcagcc caacgccgca ttagccaggc aagataataa
    38401 accaaacgca tggcgcgtaa aggttcaatc agtccgattt cagcggtgtc gaactcgcta
    38461 aattcttcat aagcttcaat aatagtttcc agttgcatcc gctgctcggc tttatcgcca
    38521 ttgagcaaca tccacaaatc ctgaatagct ggaccattac gtgcatcatc cagatcaaca
    38581 aacattggac catcgcgcca gagaatattc ccggcgtggc agtctccatg tagccgcaga
    38641 acggtgaaat cttcccgcca gtgcgctgta acggcggcga tcagctcatc tgtcgctttc
    38701 aggaatgccg ctttcaaccc ggaaggtatc agtgtagcgt cctcaaacag cttgcgtggc
    38761 tcaatgagat attcattcag accgatggtc gggcgatgga taaaaagctg tttgcgcccc
    38821 gtctggtgca tacgccctaa ataacgccca accgcttcca tctgatcgat attatcagct
    38881 tcgaactggc gaccaccgac gcttggaaaa acagcgaaat aaaatccctg atgattcaat
    38941 aaagtctgac cgttaaaggc cacaggtgct gcgaccggaa cttcatcatt taccaactgc
    39001 aacgcaaatt gatgttcttc gaggatttga tcggctgtcc aacgttcagg gcgataaaat
    39061 ttgacgacaa aacgtcgacg atcttcgtcc tgaaattgat agacacggtt ttcatagctg
    39121 ttaagcgggg taagaccgga atccacccgg atcccttgct caaatagagc atccatgatg
    39181 gtatccgggt gtagtgtctg gaaagtaaaa gcgctgttgt tcatcccatc atccggaaaa
    39241 cattacgaat gatgaaggat atcatttcac gatgattttg gtggcaccgc ttacaagctt
    39301 ttactcttta attacgccgc gagcgcgtaa cagcgccgtt ttgaagtctt cttcataatc
    39361 tttctgcaaa cccgggatca ccgcatcttt cgcggaatca cgcattttca ggtgatagat
    39421 cagaatgtca tccgtcaaat ccgccagttc gccgtcaaaa cctgactctt tcgccagttt
    39481 ctgcaaaaat tgcaacaggt taaggtctgg ctctttttgc caggctggct ggaggagttc
    39541 aataacttca ttcagacgtt tacttttcat agtaatgctc cttactcttg agacagacac
    39601 gttagcaggg tcaatcccac aataaaagag gcgatatcgg tgaatctgat gacgacgata
    39661 acaggcgttg tgctggcagg cggtaaagcc agacgaatgg gcggcgtaga taaaggattg
    39721 cttgaattaa acggcaaacc attatggcaa catgtcgctg acgcgcttat gacgcagctc
    39781 tctcacgtcg tggttaatgc caatcgccat caggaaatct atcaggcaag cggtctgaaa
    39841 gtgattgaag attcactggc ggattaccca ggccctctgg caggaatgct ttcagtaatg
    39901 cagcaggaag caggcgagtg gtttttgttt tgtccgtgcg atactcctta tattcccgag
    39961 gatttagcag cccggcttaa tcatcagcgc aaagatgcgc ctgtcgtgtg ggtccacgac
    40021 ggtgaacgcg atcacccgac tattgctctg gtaaaccgcg caattgagcc tttattactg
    40081 gaatatctgc aagcaggaga acgccgggta atggcattta tgcgtctggc tggcggtcat
    40141 gcggttgatt tcagcgataa taaagatgca tttattaacg tgaatacgcc agaggagctt
    40201 gcccgatggc aggaaaaacg atgataccgt tactcgcctt tgccgcgtgg agtggtaccg
    40261 gaaaaactac gcttttgaaa caactgatcc cggcattatg cgccagaggg atccgtccag
    40321 ggctgattaa gcatacgcac catgatatgg atgttgataa gccaggcaaa gatagctatg
    40381 agctgcgcaa ggctggcgcg gcacaaacca tcgttgccag ccagcaacga tgggccttga
    40441 tgacggaaac accagacgaa gaagagctgg atctacagtt tctcgcaagt cgaatggata
    40501 cctcaaagct ggatttgatt ctggtcgaag ggtttaagca tgaagagatc gcaaagattg
    40561 tgctgtttcg cgatggagcc ggacatcgac ctgaagaatt agtgatagac aggcatgtta
    40621 ttgctgtagc cagtgatgtg ccgcttaatc ttgatgtcgc attactggat atcaatgatg
    40681 ttgaggggct ggctgatttt gtggtggagt ggatgcaaaa gcagaacgga taagcaagag
    40741 taggctgga
//
LOCUS       DAZGLJ010000019        40230 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000019 DAZGLJ010000000
VERSION     DAZGLJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.019"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(120..833)
                     /gene="lpxT"
                     /locus_tag="ACHK49_000915"
     CDS             complement(120..833)
                     /gene="lpxT"
                     /locus_tag="ACHK49_000915"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO9261602.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(872..1858)
                     /gene="yeiR"
                     /locus_tag="ACHK49_000916"
     CDS             complement(872..1858)
                     /gene="yeiR"
                     /locus_tag="ACHK49_000916"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO9261603.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(1976..3442)
                     /locus_tag="ACHK49_000917"
     CDS             complement(1976..3442)
                     /locus_tag="ACHK49_000917"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO9261604.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLVEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHQGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            complement(3665..4237)
                     /gene="yeiP"
                     /locus_tag="ACHK49_000918"
     CDS             complement(3665..4237)
                     /gene="yeiP"
                     /locus_tag="ACHK49_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO9261605.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            4392..4646
                     /locus_tag="ACHK49_000919"
     CDS             4392..4646
                     /locus_tag="ACHK49_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO9261606.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(4643..5824)
                     /gene="setB"
                     /locus_tag="ACHK49_000920"
     CDS             complement(4643..5824)
                     /gene="setB"
                     /locus_tag="ACHK49_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO9261607.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            6192..7322
                     /gene="fruB"
                     /locus_tag="ACHK49_000921"
     CDS             6192..7322
                     /gene="fruB"
                     /locus_tag="ACHK49_000921"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO9261608.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQLAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFTEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIA
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            7322..8260
                     /gene="fruK"
                     /locus_tag="ACHK49_000922"
     CDS             7322..8260
                     /gene="fruK"
                     /locus_tag="ACHK49_000922"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO9261609.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            8277..9968
                     /gene="fruA"
                     /locus_tag="ACHK49_000923"
     CDS             8277..9968
                     /gene="fruA"
                     /locus_tag="ACHK49_000923"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO9261610.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(10061..10207)
                     /locus_tag="ACHK49_000924"
     CDS             complement(10061..10207)
                     /locus_tag="ACHK49_000924"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9261611.1"
                     /translation="MPELQKRGICDGMHRTGTLKKGRKESALEEITLVFDERFRFLLK
                     DKTI"
     gene            10392..11333
                     /locus_tag="ACHK49_000925"
     CDS             10392..11333
                     /locus_tag="ACHK49_000925"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO9261612.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRGDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            11321..12259
                     /gene="psuG"
                     /locus_tag="ACHK49_000926"
     CDS             11321..12259
                     /gene="psuG"
                     /locus_tag="ACHK49_000926"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO9261613.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            12353..13603
                     /locus_tag="ACHK49_000927"
     CDS             12353..13603
                     /locus_tag="ACHK49_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO9261614.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(13671..14369)
                     /locus_tag="ACHK49_000928"
     CDS             complement(13671..14369)
                     /locus_tag="ACHK49_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO9261615.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            14499..15440
                     /gene="rihB"
                     /locus_tag="ACHK49_000929"
     CDS             14499..15440
                     /gene="rihB"
                     /locus_tag="ACHK49_000929"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO9261616.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            15540..16790
                     /locus_tag="ACHK49_000930"
     CDS             15540..16790
                     /locus_tag="ACHK49_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO9261617.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(16846..17934)
                     /locus_tag="ACHK49_000931"
     CDS             complement(16846..17934)
                     /locus_tag="ACHK49_000931"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO9261618.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(17938..18795)
                     /gene="nfo"
                     /locus_tag="ACHK49_000932"
     CDS             complement(17938..18795)
                     /gene="nfo"
                     /locus_tag="ACHK49_000932"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO9261619.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(18869..19918)
                     /locus_tag="ACHK49_000933"
     CDS             complement(18869..19918)
                     /locus_tag="ACHK49_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO9261620.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            20017..20898
                     /gene="yieE"
                     /locus_tag="ACHK49_000934"
     CDS             20017..20898
                     /gene="yieE"
                     /locus_tag="ACHK49_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO9261621.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIKDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            21103..22572
                     /gene="lysP"
                     /locus_tag="ACHK49_000935"
     CDS             21103..22572
                     /gene="lysP"
                     /locus_tag="ACHK49_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO9261622.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            22865..24856
                     /gene="cirA"
                     /locus_tag="ACHK49_000936"
     CDS             22865..24856
                     /gene="cirA"
                     /locus_tag="ACHK49_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO9261623.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(24888..25724)
                     /gene="yeiG"
                     /locus_tag="ACHK49_000937"
     CDS             complement(24888..25724)
                     /gene="yeiG"
                     /locus_tag="ACHK49_000937"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO9261624.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTIKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            25982..26650
                     /gene="folE"
                     /locus_tag="ACHK49_000938"
     CDS             25982..26650
                     /gene="folE"
                     /locus_tag="ACHK49_000938"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO9261625.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            26667..27824
                     /gene="yeiB"
                     /locus_tag="ACHK49_000939"
     CDS             26667..27824
                     /gene="yeiB"
                     /locus_tag="ACHK49_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO9261626.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIAQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            27966..29006
                     /gene="galS"
                     /locus_tag="ACHK49_000940"
     CDS             27966..29006
                     /gene="galS"
                     /locus_tag="ACHK49_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO9261627.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            29286..30284
                     /gene="mglB"
                     /locus_tag="ACHK49_000941"
     CDS             29286..30284
                     /gene="mglB"
                     /locus_tag="ACHK49_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO9261628.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            30345..31865
                     /gene="mglA"
                     /locus_tag="ACHK49_000942"
     CDS             30345..31865
                     /gene="mglA"
                     /locus_tag="ACHK49_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO9261629.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDISFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            31881..32891
                     /gene="mglC"
                     /locus_tag="ACHK49_000943"
     CDS             31881..32891
                     /gene="mglC"
                     /locus_tag="ACHK49_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO9261630.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(32962..34197)
                     /gene="preA"
                     /locus_tag="ACHK49_000944"
     CDS             complement(32962..34197)
                     /gene="preA"
                     /locus_tag="ACHK49_000944"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO9261631.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(34191..35429)
                     /gene="preT"
                     /locus_tag="ACHK49_000945"
     CDS             complement(34191..35429)
                     /gene="preT"
                     /locus_tag="ACHK49_000945"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO9261632.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(35748..35987)
                     /locus_tag="ACHK49_000946"
     CDS             complement(35748..35987)
                     /locus_tag="ACHK49_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO9261633.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(35990..36709)
                     /gene="sanA"
                     /locus_tag="ACHK49_000947"
     CDS             complement(35990..36709)
                     /gene="sanA"
                     /locus_tag="ACHK49_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO9261634.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(36859..37743)
                     /gene="cdd"
                     /locus_tag="ACHK49_000948"
     CDS             complement(36859..37743)
                     /gene="cdd"
                     /locus_tag="ACHK49_000948"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO9261635.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(37873..38568)
                     /locus_tag="ACHK49_000949"
     CDS             complement(37873..38568)
                     /locus_tag="ACHK49_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO9261636.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(38565..38963)
                     /locus_tag="ACHK49_000950"
     CDS             complement(38565..38963)
                     /locus_tag="ACHK49_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO9261637.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            39202..40152
                     /gene="dusC"
                     /locus_tag="ACHK49_000951"
     CDS             39202..40152
                     /gene="dusC"
                     /locus_tag="ACHK49_000951"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO9261638.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
BASE COUNT         9931 a         9863 c        10512 g         9924 t
ORIGIN      
        1 atgcaatcca ttcatttact tagagaatga tttttgcgcg cgatggatga aagcacggtg
       61 cgcatcatca ggattatcct cactataaaa ataagcctga tgatgttaat tactgtgagt
      121 tatttgtttt ggaaatgttt gttttttccc ggtagtgatt tgtcaaaaaa ggtgattaat
      181 cgatcactta atggcgtcag caacacccag ggcaaaccga tcaatatcac ggtcatcgaa
      241 ccgacaatga tgtcagtaaa ccagtgtgcg ccaatcatta ctctgggaaa tgcaaaaacc
      301 acaaaaataa taagggcgat aaggcctgca actttgccga aataacgcca catgaatgcc
      361 gaaaaaataa gcagcatcat gccgtgatcg ccgggaaagc tatctcgtga ggcatctttc
      421 gtgggaacag agagcagttc gctgacgcgg ttaatatcgg taaaagtcaa tgttgggctg
      481 gcccgtttta caggaattaa tgcctgaccc agctggttta ataccactgc agttaatagc
      541 atgaccagac ccataatcac gatacgtcgt ctgccagggg cgttttcttt cagccagaaa
      601 ctcagcatca acattcccat cgccagcaga gaacaaccgt cgaaagcgcg attgttggtc
      661 aatgcaacca gccacaaaaa ggccttactt tcgaccagtt tctgattaaa gaaataaaaa
      721 atatccgcat caatcggcaa ccagaatcca tgattaacgg gaatatacca ggaaagaaac
      781 agcgcgaggc cgacaatatt caacaacact atttgcggca aatttttaat catattttct
      841 ctaattatct tactgaaaaa cgcaggcaac cttacgcggt agtcgctaaa cgaagcttca
      901 acagcgcgct ctgcaaagca ttccagtcag cttcgctgct ggaaatcagc tcaatacggc
      961 tgtccggtgg cgcaacgttt tgcgtttcaa tgtgcaggtc atcgccctga cggttgattc
     1021 gtaccagccc ttcgggaata cgcagcacgc ctttgacgcg ttccaccggt gcaagtcgcg
     1081 cccattccag aatgccaatg gtgtcgaata ccgtatcagc gtcgaatatc cagccgcagg
     1141 cctgatatcc ttgcccgctg ttcagactgc gacgccagcg ttgatgctct ggcaggctta
     1201 acgctgctaa cccttttttc acgacatgct gatgagaatg cgcggcgctg gcgggcaact
     1261 cggctaaatt gcgacgcggc aaatccagaa gatgaccgtc aactttccca tgctcactgt
     1321 gaattaattg tcgatcgcca ccattttgct gccaccaacg ctgtagcgct tgctcacttt
     1381 cgggcgtcgc acggtcggat ttattggcga caatgatgtc tgcggcagcc agctggtcac
     1441 ggaagttttc attgctggcg cttttttcgt ccagcagcaa gcgcggatcg agaatgcaca
     1501 aggtggcgcg cagatctatc cacggttcat agactggtgc ggttaacaga tcgaggatct
     1561 gtttcggatg gcccagcccg gtcggctcta tcaacaagcg gtctggtttt ccctgacgca
     1621 gtaaggtatt caaccctacc tgcatgggta aaccattaac gcagcacatg cagccgccgg
     1681 ggatctcttt cagcaatgcg ccgctatcgg cgagcaaagc accatcaatt ccgacttccc
     1741 caaattcatt aaccagtacc gcccattttt cgttgggatc tttatgggct aacagatgaa
     1801 gaatcgacgt ggttttcccg ctgccgagaa aaccggtgat gaggttggtc ctggtcacaa
     1861 cggctccgca atactctggt aagttaactg agcgttaatc ctggctaaaa aaagcagaaa
     1921 agagaagggg agagcggacg aaggtgagct tcatccgctt taaccgcaga aatcgttaaa
     1981 ttttgagggt gtttaatagc gcctgatgtg cgccgaattg tacgatttgt tgccaggctt
     2041 gttcgatggc ctgcacaaaa tgcgggttat ctggcagatc atcaccgaaa acttcacgca
     2101 gggaaagcag ggcggttacg cgttgttcac tgctgctgcc cgcaacaagt tcgcgaattt
     2161 tatcgctaag cggatcgcga acatcaatgg catttccggc atcatcaacg ccgctgacgt
     2221 aacgcatcca gcctgcaacg cctaatgcca gcaacgacca gtccgtttcg cgcccctgat
     2281 gtatgcgaat acctgccagc atgcgttgcg gtaatttctg gctgccatcc atcgcgattt
     2341 gccaggtctt atgtttcagc gccggattag caaaacgtgc aattaactta tccgcatatt
     2401 gtgttaaatc gacatcttta atttgcagtg tcggcgcttg ctcatccagc attaatgttc
     2461 tggcggcatg gcgaaatgcg cgatcctgca tgcaatcact gatatgggcg aatcctgaga
     2521 ggtaacccag ataagcgaga aaagagtggc tgccattaag catccgcagt ttcatctctt
     2581 cccatggcag gacatcattc accatttgta cacctgcgac ttcccaggca ggacgcccag
     2641 cgacgaagtt atcttccacc acccactgga taaacggttc gcagctaatc gcgcagggat
     2701 cattcacccc cagatgctgg ctgatttcca ccagtgattc gtcggttgca gccggaacaa
     2761 tgcggtcgac catggttccc ggaaaactga cgtgctcttt tatccacccg gcgagttctg
     2821 gcgaacgttt ttctgccatt cccagcaccg cgtttttcac cacatgacca ttgtcgggaa
     2881 tattgtcgca ggagagcacg gtaaacggtg taaggccgcg ctcacggcgg cgtttcagtg
     2941 cttcgacgag aatacccggt gcggagtgag gttcttcagg ggtttgtaga tcgtgaataa
     3001 tccgcggatt actggtgtcg agtgcaccgg tggccgggtc aatacaatag cctttttcgg
     3061 taatcgtcag ggaaacaatt gccacctgtg gctcgcaaaa tttctcaata attgccgcta
     3121 aggaatccag ttttgcatta aggcattcgt gaacggcacc gacaattatc acctgattgc
     3181 cgtccgcacc tttctccagc acggtatata aatggttctg tgcgcggagc tggctcatca
     3241 gttgatcacc gctgaacaag ctgatttcac agatccccca gtcgccgccc tgggcattca
     3301 gcacacgatc ggtcagtaac gcctgatgag cgcggtgaaa ggcgccaaag ccaaaatgaa
     3361 cgatacgtga ttgcaactgc tggcgatcgt agcgtggagc gtgtacatga tgcgggagcg
     3421 taacggaggc aattgtcttc ataggagtca cgtcttagta acgataaatt aacaatgaca
     3481 gtgtaaagtt atatgacagc aattgaaatt ggtctggatt gttaatgcga tgaagtgtga
     3541 gggcgatcaa tcaacgagtg aaaattgagg gcgtaaggtg ttcaggaagc gcaaaataga
     3601 agattggtat aataaattaa aaaagggaca gcctgagcta tcccttttct gcatgcggct
     3661 gaagttagtc agcacgcccc atataacggc gttcttcgat atggatacga attttttcgc
     3721 ccgggcttaa gtattctggt acctgaatca ccagaccagt gctcaatgtc gccggtttgt
     3781 tacgggcgct ggcagatgcc cctttgatgc ctggtgcggt ttcaacgatt tccagatcaa
     3841 cggtctgcgg cagctcaagc gccagcagtt gaccgtccca ggtcagcacc tgcatgtccg
     3901 gcatgccgcc ctctggcata aacagcaact cttcttcaat ctgatctttg gtgaaggtat
     3961 acggggtata gtcttcttta tccataaaga catattcgtt gccatcgaca taggagaagt
     4021 caacgtaacg gcgggtcagc gtcacggtgt caacgatatc atcacctttg aaacgctctt
     4081 ctactttcag cccggtacgg acatcagaaa aacgcatttt gtacagcgta gcggcaccgc
     4141 gggcagtggg cgactgaata tcaatatcct taaccagcag cagtttgccg ttgtaattca
     4201 gtaccatacc ttttttaatt tcgttcgctc ttggcatcga aaaattcctg tagtcgaaaa
     4261 tgtcaaaaat atcgcgacaa agtactcgcg acgggccttt cgggcaagtg gtattcgcac
     4321 ttttgctggt gcaaaaaagg tggtactgtg cgcgctcatc aatccggtgg ttaaccttaa
     4381 gagaacaacg tatggaatgc cgtccgggtt gcggggcgtg ttgcactgcc ccttcaattt
     4441 ccagccctat tcccggtatg ccagatggca agcccgccaa tacgccctgc attcagcttg
     4501 atgaacagca acgctgtaaa attttcacct cgccactgcg accgaaagtc tgtgcaggct
     4561 tacaagccag cgctgaaatg tgcggtaact cgcgtcagca ggccatgacc tggcttatcg
     4621 atctggaaat gctgaccgcg ccttaaacat ctttaatccg cagtaagcaa aacagagtgg
     4681 cgataatcat caccatcgca aaccagaaca cagcgtgata attccagatc tcggcgacga
     4741 tgcccgccac tgatcctgcg atgatccagc ccacgcgcga agtgttggta tagagcgtgg
     4801 tggctgaacc cgcctgaccg ggcatcagat cctgaaaata gagcatcccg atgccgccca
     4861 gaatgccaat aaaaatagca tttagcagct gcaagcccaa cagaatgaca ggtgaatgcg
     4921 ccatcagcat tcctgcgtaa aaacagacgc cacccacggc agcaacgcgc attaagaaac
     4981 gcttacccag acgtttggcg aaatatccgg caatcaacat cgtcgggatt tccagcccgg
     5041 cggcggtccc catcatcaca ccggccagtt tctcgggaag atgcagttcg ttgataataa
     5101 atagcggcat gttgatgatg tacaggctgt tcgagcccca cattaatgta caaatgacaa
     5161 acagcagtag cgtatcgcga cggttacggc gcggcgcttc aaccgtgccg gtcgccagcg
     5221 gaagctcttt ttgcatcgac ggtaaaaaca gccacaccat cacgccgcaa acaataaacg
     5281 ctaccgctgc gctcagatac attaccgtaa agctgaaacc catcgctaac gcataagcca
     5341 gcggtgggcc aatgacccat gccagtgaaa cctgagcgcg taaaaaagag ctgaacatca
     5401 ccgcctcacg tccggttttg tcggcatgtt cacgggcaag ggcaaacatt tgcgggttag
     5461 cggtcgagcc aaagctgcta agaaagacgc caacgaatag caaaacaaag tagttgcgat
     5521 tccaggcaaa aagggtgcag gccagcacgc ctaacaggca gcaaaagaca atcagcgatt
     5581 tgcgatcgcc gcgcttatca gagcgcccgg cgagaaactg acttaccaga atcccaatga
     5641 cagcgctgcc ggtaaagaag aatcccacca tcgccggacg ggcatgtact tcatcggtaa
     5701 gaaaaatact gagtgtcggg gtttgcagag cgcccgcaat accggtgaga aaggcaacga
     5761 ttaaaaacgc cgtcgaggtc aggtcaaacg atttcgcgct ggagactgcg ggggagttat
     5821 gcatgctggt cgatcactgg aagaggaaga cgcggagttt acgctgcttg tcagaaaata
     5881 aacagacagc gaaatcaaaa gcgccacgaa taatcaaaat gccatttcac tgttgctgat
     5941 tggctgctga cgctgctgaa atgaaattgc tgataacgga ttttcccatc agcaattagg
     6001 aaaaatgaca aaaaattgtg cagcacatca aacttttgct cataacttta cggctttcct
     6061 tgcgtgctga aacagaagta ttatgctttc ttgaaacgtt tcagcgcgat cttgtcttta
     6121 accctaagcc aggttggcgc tttttttctc atagaagctg aatcgtttca attcagcaag
     6181 agaggagaac tatgttccag ttatccgtac aggacatcca tccgggcgaa aaggccggag
     6241 acaaagaaga ggcgattcgc cagctcgctg cggcgctggt gcaggccggt aacgtagcag
     6301 aaggctacgt caatggcatg ctggcgcgcg aacagcaaac ctcaacgttc ctcggcaatg
     6361 gtattgctat tccacacggc actaccgaca cccgcgatca ggtgctgaaa accggcgttc
     6421 aggtatttca gttcacggaa ggcgtcacct ggggtgacgg tcaggtagcg tacgtagcga
     6481 tcggtattgc tgccagctcg gatgaacatc tgggcctgct acgccagctg acccacgtac
     6541 tgagcgatga ttccgttgct gaacaactga agtcagcaac aacagcagaa gaacttcgcg
     6601 cattgctgat gggcgaaaag cagagtgagc agctgaagct cgacaacgaa atgctgacgc
     6661 tggatatcgc cgccagcgat ctgctgactc ttcaggcgct gaacgctgcg cgtctgaaag
     6721 aggcgagggc agttgacgcc actttcgtca ccaaagccat caatgaacaa ccgctgaacc
     6781 tcggacaggg tatctggctg agcgatagcg ccgaaggcaa tctgcgtagc gcgattgcgg
     6841 taagccgtgc ggcaaatgct tttgatgtgg acggcgaaac ggcagccatg ctggtgagtg
     6901 tggcgatgaa tgacgatcag cccattgcgg ttcttaagcg tctcgctgat ttgttgctcg
     6961 acaataaagc tgaccgcttg ctgaaagcgg atgcggcaac gttgctggcg ctgctgacca
     7021 gcgatgatgc gccgaccgac gacgtgttaa gcgcggagtt tgtggtgcgc aatgaacacg
     7081 gcctgcatgc tcgtccaggt accatgctgg tcaacaccat taaacaattt aacagtgata
     7141 ttaccgtgac aaaccttgat ggcaccggca aaccggcaaa cggacgtagt ctgatgaaag
     7201 ttgtggcact tggcgttaag aaaggtcatc gcctacgctt taccgcccag ggtgcagatg
     7261 ctgaacaggc gctgaaagca atcggcgacg ctatcgctgc tggtcttggg gagggcgcat
     7321 aatgagcaga cgtgttgcta ctatcaccct taatccggct tatgaccttg ttggtttctg
     7381 cccggaaatt gaacgcggcg aagtgaacct ggtgaaaacc accggtctgc atgcggcggg
     7441 taaaggcatc aacgtggcca aagtattaaa agacctggga attgatgtca ccgttggcgg
     7501 cttcctgggt aaagacaatc aggatggttt tcagcaactg ttcagcgagc tgggcattgc
     7561 caaccgtttc caggttgtac aggggcgcac ccgaattaac gttaagctga cggaaaaaga
     7621 cggcgaagtg accgacttca acttctcggg ttttgaagtc acccccgccg actgggaacg
     7681 ctttgtgact gattctctga gctggctcgg tcagttcgat atggtctgtg tcagcggaag
     7741 cttaccgtca ggcgtcagcc cggaagcgtt caccgactgg atgactcgcc tgcgtagtca
     7801 gtgtccttgc attatctttg atagtagccg tgaagcgtta gtagcaggtt tgaaagcggc
     7861 accgtggctg gtgaaaccta accgccgcga gttggaaatc tgggcaggcc gtaaactgcc
     7921 tgaaatgaaa gatgtgattg aagctgcaca tgcgctacgt gaacaaggta ttgcgcatgt
     7981 tgttatttca ctgggtgccg aaggcgcgct ttgggttaat gcctccggcg aatggatcgc
     8041 caaaccaccg tcagtcgatg tcgtaagcac cgttggcgca ggggattcta tggttggtgg
     8101 cctgatttat ggcttgctga tgcgtgaatc cagtgaacac acactgcgtc tggcgactgc
     8161 tgttgcagcc ctggcggtaa gtcaaagcaa tgtgggtatt accgatcgtc cgcagttggc
     8221 cgcaatgatg gcgcgcgtcg acttacaacc ttttaactga cagcaggaga ggcataatga
     8281 aaacgctgct gattattgac gctaatctcg gtcaggcacg cgcctatatg gcgaagaccc
     8341 tgctgggcgc ggcggcgcga aaagcaaaac tggaaatcat cgacaatccg aacgacgcag
     8401 aaatggcgat tgttctcggt gattccatcc cgaatgacag cgcgctgaac ggtaaaaatg
     8461 tctggctggg tgatatttcc cgggcagttg cgcaccctga gctgttcctg agtgaagcca
     8521 aaggccatgc gaaaccttac actgcgccgg tcgctgcgac agcaccggtt gccgccagcg
     8581 gtccgaaacg cgtagttgcg gtgactgctt gcccgactgg cgtagcacac acctttatgg
     8641 cggctgaagc cattgaaacc gaagcgaaaa aacgtggctg gtgggtgaaa gttgaaaccc
     8701 gtggttctgt tggtgcgggt aatgcaatca cccccgaaga agtagccgca gcggatctgg
     8761 tgattgtggc ggcagatatc gaagtggatc tggcgaaatt tgctggtaaa ccgatgtatc
     8821 gtacctctac cggtctggcg ctgaagaaaa ccgcgcagga actggataaa gcggttgctg
     8881 aagcaacgcc gtatgaaccg gcgggcaaag ctcaaacggc gacctctgaa ggtaagaaag
     8941 agagtgcagg cgcataccgt cacttgctga cgggcgtctc ttacatgctg ccgatggtcg
     9001 ttgcaggtgg tctgtgtatc gcgctttctt ttgcttttgg tatcgaagcg tttaaagagc
     9061 cgggtacgtt ggcagcggcg ctgatgcaga ttggtggtgg ttcagccttt gcgctgatgg
     9121 tgccggtact ggcaggttac attgccttct ccattgccga tcgtccgggt ctcacgccgg
     9181 gtctgattgg cggtatgctg gcggtcagca ccggttctgg cttcattggc ggtattattg
     9241 cgggcttcct ggctggttac attgcgaagt taatcagtac gcaactgaaa ctgccacaga
     9301 gtatggaggc gctgaaaccg atcctgatca ttccgctaat ttccagtctg gtggtcggtc
     9361 tggcgatgat ctacctgatc ggtaaaccag ttgctggcat tctcgaaggg ttgactcact
     9421 ggctgcagac catggggact gcgaatgcgg ttctgctggg ggcgatcctc ggtggcatga
     9481 tgtgtactga catgggcggt ccggtaaaca aagcagcgta cgcattcggt gtgggtctgc
     9541 tgagtactca aacctatggc ccgatggcgg cgattatggc ggcaggtatg gtgccaccgc
     9601 tggcaatggg tctggcaaca atggtggcgc gtcgcaaatt cgacaaagcg cagcaggaag
     9661 gtggcaaagc cgctctggta ttgggactgt gcttcatttc ggaaggtgca attccgtttg
     9721 ctgctcgtga tccgatgcgt gtgctgccgt gttgtatcgt tggtggggcg ctgactggcg
     9781 caatctcaat ggcgattggt gcgaaactga tggcaccgca cggtggtctg tttgttctgc
     9841 tgatccctgg cgctattacg ccggtactgg gttacctggt agcaattatt gccggtacgc
     9901 tggtggcggg tttggcctat gccttcctga aacgtccgga agtggacgca gtagcgaaag
     9961 ccgcgtaata aaaggtgtgt tacagggcag aaatttactg ccctgtatca ttggatgagc
    10021 gcaaagagaa tgaccggttt gtgaatgtaa ttttatgaat ttaaatggtt ttatctttca
    10081 gtagaaacct gaaacgttca tcaaacacta aggttatttc ctcaagggca gattcttttc
    10141 tgcccttttt tagtgttcct gttctgtgca tcccgtcaca aattcctcgc ttttgtaatt
    10201 ctggcattta atcgcatttt gtgagcctgc tcaaatctgc ccgctttgcc tctctggcgg
    10261 caatttactt tacgtaaaac atacatacta tgagcacatg tttaaatatg aacacatgtt
    10321 taaactgtct ttgcagtatc tatattcttg tggattgaca gggttggtaa ttttttacca
    10381 gaggacgtgc tatgcgcgaa aaggattatg tcgtaattat aggttcggcg aatattgatg
    10441 tcgccggata ttcacatgaa tcattaaatt atgcggattc aaatccaggt aaaataaaat
    10501 ttacacctgg tggagtaggg cgaaatattg cacaaaacct ggcgttgctg ggtaacaaag
    10561 cctggttact gagcgcagta ggtagtgatt tttatggtca atcgctgcta acgcaaacca
    10621 atcaatctgg cgtttatgtc gataaatgcc tgattgtgcc gggagaaaat acgtcgagtt
    10681 atttatcatt actcgataat accggtgaaa tgctggttgc tataaatgac atgaatatta
    10741 gcaacgctat tacagctgaa tatctcgcac agcaccgtga atttattcag agggcaaagg
    10801 tcattgtcgc ggactgtaat atcagtgaag aggcactggc atggattctg gataatgctg
    10861 ccaacgtacc cgtatttgtc gatccggttt cagcgtggaa atgtgtcaaa gtgcgcgacc
    10921 gtctaaatca gatccacact ctgaagccaa accgccttga agcggaaaca ctgagtggga
    10981 ttgcgctgtc agggcgtgga gatgtggcaa aagttgctgc ctggttccat caacatggcc
    11041 tgaaccgact ggtattgagc atgggcggcg atggcgttta ttacagtgat atcagcggtg
    11101 aaagtggctg gtctgccccg atcaaaacca atgttattaa tgttaccgga gcgggcgatg
    11161 ccatgatggc gggacttgct tcgtgttggg tagacggaat gccgtttgcc gaatctgttc
    11221 gtttcgcaca gggatgttcg tcaatggcgc tctcctgtga atacaccaat aaccccgatt
    11281 tatcgattgc caacgttata tcgttagtgg agaacgcaga atgtctgaat taaaaatttc
    11341 ccctgaatta ttacaaattt ccccggaagt gcaggacgct ttaaaaaaca aaaaaccggt
    11401 tgtggcgctg gaatcgacca ttatttctca cgggatgccg ttcccacaaa atgcccagac
    11461 cgcaattgaa gtagaagaaa ctattcgtaa acagggcgca gtacctgcca ctatcgccat
    11521 tattggcggc gtgatgaaag tgggtttaag caaagaagaa attgaattac tgggtcgtga
    11581 agggcataac gtgaccaaag ttagtcgtcg cgatttacct tttgttgttg ccgccggaaa
    11641 aaatggcgca accactgtgg cttcaacgat gattattgcg gcgcttgccg gaattaaagt
    11701 atttgccacc gggggaattg gtggtgtgca tcgcggggcg gaacatacct tcgatatttc
    11761 tgccgatttg caagaactgg caaatactaa tgtcaccgtt gtttgtgccg gggcgaaatc
    11821 tattctcgat ttaggattaa caacagaata tttagaaacc ttcggtgtgc cgttaattgg
    11881 ctatcagaca aaagcgctgc ctgcgttttt ctgccgtacc agcccatttg aagtcagtat
    11941 tcgccttgat agtgccacgg aaatcgctcg tgcaatggca gtgaaatggc aaaccggttt
    12001 gaacggcggc ctggtagtgg cgaatccgat cccggaacag tttgcgatgc cggaggaatc
    12061 tatcaatgca gccatagatc aagccgtcgc cgaagccgaa gagcagggcg ttattggtaa
    12121 agaaagtaca ccgttcctgc tggcgcgcgt tgctgagctg accggcggtg acagcctgaa
    12181 atccaatatc cagctggtgt tcaacaacgc cattctggcg agtgaaattg ccaaagaata
    12241 ccagcgtctc gcgggttaat ttctgacggg cagaaagccg tcgcccgtac tcatcaagca
    12301 taccaaaacc tggcgctgac gccgggttta ctggcatgaa gggaaaatca ttatggatat
    12361 aatgagaagt gttgtgggga tggtggtgtt actggcaata gcatttctgt tgtcagtgaa
    12421 taaaaagagc atcagtttgc gcacggttgg agccgcactg ctgctgcaaa tcgctattgg
    12481 tggcatcatg ctctacttcc caccgggaaa gtgggcagta gaacaggcgg cattaggcgt
    12541 tcataaagtg atgtcttaca gtgatgccgg tagcgccttc atttttggtt cgctggttgg
    12601 gccgaaaatg gatgtcctgt ttgacggtgc gggttttatc ttcgcctttc gcgtacttcc
    12661 ggcgattatt ttcgttactg cgctcatcag tctgctgtac tacattggcg tgatggggct
    12721 gctgattcgc atccttggca gcattttcca gaaagctctt aacatcagca aaatcgaatc
    12781 ttttgtcgca gtcacaacta ttttcctcgg gcaaaatgag atcccggcga tcgttaaacc
    12841 gtttatcgat cgcatgaatc gcaacgagtt gtttaccgca atttgtagcg ggatggcgtc
    12901 cattgctggt tcgatgatga ttggttatgc cggaatgggc gtaccaattg actacctgtt
    12961 agcggcatcg ctgatggcga tcccaggtgg tattttgttt gcacgtattc ttagcccggc
    13021 caccgagcct tcacaggtca catttgaaaa tctgtcgttc agcgaaacgc cgccaaaaag
    13081 ctttatcgaa gcggcggcga gcggtgcgat gaccgggctg aaaatcgccg ctggtgtagc
    13141 gacggtggta atggcgtttg tcgcaattat tgcgctgatc aacggcatta tcggcggaat
    13201 tggtggctgg tttggtttcg ccaatgcctc tctggaaagt atttttggct atgtgctggc
    13261 accgctggcg tggatcatgg gtgtggactg gagtgatgcc aatcttgcgg gtagcctgat
    13321 tgggcagaaa ctggcgatta acgaattcgt cgcttacctg agtttctccc catacctgca
    13381 aacgggcggc acgctggaag tgaaaaccat tgcgattatc tcctttgcgc tttgtggttt
    13441 tgctaacttt ggttctatcg gtgttgtcgt tggcgcattt tcggctattt cgccaaaacg
    13501 cgcgccggaa atcgcccagc ttggtttacg ggcgctggca gcagcaacgc tttccaacct
    13561 gatgagtgcg accattgccg ggttctttat tggtttagct tgagtttcat tgccggatgt
    13621 acgcgcttat taagtgttgc atccggcaat cgcgctattg caaataattt ttactgcatc
    13681 atcccggaga atttattctc cgggtccatc tccagcgcca gtcctgacaa ctgctttctg
    13741 tttttaatta gatacccttt cttgctttta attaataaac cgtcgtgaat gaattgcgcg
    13801 agaacatata aaagatgtcg ataagaaacg cctaaatact ctgccgcttg cgtatgcttt
    13861 tcgtgataaa gatcaccttc ctgcgagagt aatataaatg ctgccaggcg attcactaac
    13921 ggaaatgatt gattctgagt taaagaaaca atattacgat aatttttatg acttaaggtg
    13981 acgcagagtt ttcgtaaaaa tagcgtgtcg tttaataacc gcgggcggta atgtttcata
    14041 gggagcgcaa ggcaccaaca ctcttcaata gcctgcaccg cacgcggttc atggtctttg
    14101 tcgattaact caatctcgcc aataaaacag ggggcggcaa agaaatcgat cagcgacacg
    14161 cgaccattag ctagcgtggc gtaaagcctg gcgcggcctc gcgtcaggta aaacagccag
    14221 gacggctgtt gcccttcctg cacaatgtag tcacgcgcta aaaaatgaaa cagccgcgta
    14281 tcggctgaaa catccgttaa aaagcaatcc ttaaacgcgg attcactcat caattgctgc
    14341 ttaagatcat tattgtggat ttctttcacg atgtttatcc gctatgagat ttctcatatt
    14401 tagcatacgt acgcggttgg taagattcaa tcatcggccc ggtcgaattt tctttgctgt
    14461 agaaaaaata acgcaactgg aaacagagga aataaacaat ggaaaagaga aaaattattc
    14521 tggattgtga tccgggtcat gatgatgcta ttgcaatgat gatggcagcg aaacatccgg
    14581 caatagattt attaggcatc actattgtag cgggtaatca gacgcttgat aaaacattaa
    14641 ttaatggcct gaatgtttgc cagaaactgg agattaatgt tccggtttac gcggggatgc
    14701 cgcaacccat tatgcgtcaa caaatcgttg ccgataatat tcacggtgaa accggactgg
    14761 atggcccggt attcgagccg ctaacccgcc aggcagaaag tacccacgcg gtgaaataca
    14821 tcattgatac cctgatggca agcgatggcg atatcactct ggtgccggtt ggtccgcttt
    14881 caaatatcgc ggtggcaatg cgtatgcaac ccgcgatcct gcccaaaatc cgtgaaattg
    14941 tgctaatggg cggcgcttac ggtacaggca acttcacgcc atctgccgag ttcaacatct
    15001 ttgctgaccc ggaagcagcc cgcgtggtgt tcacctctgg cgttccatta gtgatgatgg
    15061 gcctcgatct caccaaccag accgtttgca ccccggacgt gattgctcgg atggaaaggg
    15121 caggcggccc tgccggagag ctgttcagcg acatcatgaa cttcactctc aaaacgcagt
    15181 tcgaaaacta cggcctggct ggcggcccgg tgcacgacgc cacctgcatc ggttatctga
    15241 ttaaccctga tggcattaaa acccaggaga tgtacgtcga agtggacgtc aacagtggcc
    15301 cttgctatgg gcgcaccgtc tgcgacgagc tgggcgttct tggcaagccc gccaatacca
    15361 aagtcggcat cactattgat accgactggt tctggggctt agtcgaagag tgcgtgcgcg
    15421 gctacatcaa aacccattaa gcgttgataa caagccgggc cgatagcgca agatgcaggc
    15481 ccggctattt ctgatgggca gaaagccgtc gcccaatatg acatgaagag ataatcacta
    15541 tggatgtcat gagaagtgtt ctgggaatgg tggtattgct ggcaattgca tttttgctgt
    15601 cagtaaacaa gaagaagatc agcctgcgta ccgttggcgc ggcgttagtg ttacaggttg
    15661 tgattggcgg cattatgctt tggttaccgc cagggcgttg ggtcgctgaa aaagtcgctt
    15721 ttggcgtgca taaagtgatg gcgtacagcg acgcgggtag cgcatttatc ttcggttcgc
    15781 tggtcgggcc gaaaatggac acgctgtttg atggcgcagg atttatcttt ggtttcagag
    15841 tattaccggc aattatcttc gttacagcac tggtgagtat tctctactac atcggtgtga
    15901 tggggatttt aattcgcatt ctcggcggta tcttccagaa agcgttaaat atcagcaaga
    15961 tcgagtcatt cgtcgcggtc actaccattt tcctcgggca aaacgaaatt ccggcaatcg
    16021 tcaaaccctt tatcgatcgt ctgaatcgca atgaattatt tacagcgatt tgtagtggca
    16081 tggcctcgat tgctggttcg acaatgattg gttacgccgc cctgggcgta cctgtggaat
    16141 atttgctggc ggcatcgtta atggcgatcc caggcgggat cttgtttgcc cgcctgttaa
    16201 gcccggctac ggaatcttcg caggtttcct ttaataacct ctctttcacc gaaacaccgc
    16261 caaaaagcat tattgaagcc gctgcgacag gggcaatgac cgggctgaaa atcgccgccg
    16321 gtgtagcgac agttgttatg gcatttgtcg ccatcattgc gttaattaat ggtattatcg
    16381 gcggcgttgg cggctggttt ggttttgcac atgcctcgct ggagtccatt ttaggttacc
    16441 tgttggcccc attggcgtgg gtgatggggg ttgactggag tgatgcaaat cttgccggga
    16501 gtttgattgg gcagaagctg gcgatcaatg aatttgtcgc ttatctcaat ttctcgccat
    16561 atctgcaaac gggtggcact ctggatgcta aaaccgtggc gattatttct ttcgcgttgt
    16621 gcggtttcgc taactttggt tctatcgggg tggtggtggg ggcgttttct gcggttgcgc
    16681 cacaccgtgc gccggaaatc gcccaacttg gtttacgcgc gctggcggcg gcgacacttt
    16741 ctaacctgat gagtgctact attgcaggat tctttattgg tttagcgtag tgcgcaattt
    16801 atgagccgga taagatgcgg tcaagcgtcg aatccggcaa cattgttaca ccattggcac
    16861 taatgaaagc gcgttatcgg cagacagggt agggttgttt aagctgccgc tggcgcgcga
    16921 aattgccgcg caggctaccg caaaacgggc gctgtcgcgg aaactgtatc cttcaagaaa
    16981 gctatatacc aggcccgcca taaaaccatc gtcagcacca aaactgtcta ctgtcgtatg
    17041 cgctggcgcg gtcagcaaaa attgttctcc atccttatcg ctgcaaaata cagattcatc
    17101 tttcagataa acaaaaatgc gtttaacacc ttgttgatgc agcgaattta cagctctgat
    17161 gcgatcgttg tcgtctttaa tcggttgccc ccacaaaatt tccagttcat tttgtgttgg
    17221 tttcagagtg tggatccggc tgtaccagtt ttttactttg ttcgctttaa attcggaaac
    17281 ggtatcgaca aacatcggaa tttcatcagc aatggtaaag acccactcca gggcttcagg
    17341 tgtgagatta caatcagcca gcaccacacc agcatggcga atcaagtccc gtgaagtatt
    17401 gagcagttga ggcgttagct gttgcagaat atgtgtgtca ttgattgcca ggatagtttc
    17461 ttcctgctta ttagcgatag cgagataagt tgcagtgctg tgtccatgca gacgaatgca
    17521 gtttgaaacg ttcacgcccg cgcggcgcgt ttcttccagt aatgtctcgc cgtaaaaatc
    17581 gttgccaata gctgatatta aatgcacatc ccgaccaagc agagccaggt tatgcgcgat
    17641 attacgtccg acgccgcctg cggaacagtg aacagaacct ggatgagagg ctgcttgcgg
    17701 gtaacggata tccgccattc cgcgaatatc catattgatt gcacccacaa ccacacagta
    17761 atcctgctcg gtaaggatat atcctttgcc tttaatcaaa cccttgcgca tcaaatccat
    17821 aatatgagcg gcaacacgtg agcggctgat ttgcagaata tcggcgattt cattctgttg
    17881 aatcagcggg ttacgccgta aaattttaag aatctgcttt tccctgtcgt tcatcgatca
    17941 ggccaccgct ttttcagttt gttgcgcttt cagccaggcg atctcttctg cccagatatc
    18001 cgggttgatg gtttcaagga tcagcggaat gccgtcgaaa cggtcgtcct gcatgatcca
    18061 gcggaacgca tcatgaccga tattgccttc accgaggcta tgatggcggt caacgcggct
    18121 gccaaaggtg cttttcgcat cgttaaggtg catcccgcgc agatacttaa agccaacaat
    18181 acgggcaaaa tcagcgaacg ttttttcgca ttcggctgga gtacgcaaat catacccggc
    18241 agcgaaagca tggcaggtat caatgcagac gccgacacgg gatttatctt ccacgccgtc
    18301 gataatcgcc gcgagatgtt cgaatttaaa ccctaagtta ctgccctgac cggcggtgtt
    18361 ttcaatcacc gctgttacac cttgagtttt atccagcgca atgttgatgg attcggcaat
    18421 acgcgcaagg caatcctctt ctgaaatctg catcagatgg ctgccagggt ggaagttgag
    18481 caaagaaagc cccagttgtt cgcaacgctg catttcatct ataaaggcat cgcgcgattt
    18541 ttccagagct tcagtgaccg gatgtccgag gttaatcaga tagctgtcgt ggggaagaat
    18601 ttgcgccgat gtgtagtgat atttttcaca ggccgctttg aattcatcga tggtttgcgt
    18661 cgtgagcggt gcggcacgcc actgacgttg gtttttggtg aacaaggcaa acgcggttgc
    18721 gtcgatttcg gcggcgcgaa ttgcggcatt tgccagaccg ccagcagcac taacgtgcgc
    18781 tccaatgtat ttcatgcgag gactcctgtt aaacccgctg gaggaaaacg gtaatgatag
    18841 cgggttaaca ggagcaagat gtagtggttt atgcgatgac gctttgaatc acatagttaa
    18901 tcgcaccacc accaacaatc agccaggcaa acagtaccag tgccatcagc agaggtttcg
    18961 ccccggcttt tttcagcgcg ctgacgtgag tggtcagacc cagcgccgcc atcgccattg
    19021 ccagcaggaa ggtatccagc gttaccagca tgttcaccac gctctgcggt aacaggtgga
    19081 acgagttaaa gatggcaact acgatgaaca agatggcaaa ccacggaata gtgattttgc
    19141 ttttctcgcc gctgttcgcc ccagacagct gtttaacacg cgccgccagc aggatgagga
    19201 acggagccag catcatcacg cgcagcattt tggaaataac tgctgcgttt tccgcatccg
    19261 ggctgatggc atgacctgcc gccaccacct gcgccacttc gtgcacagta gaaccaatgt
    19321 agataccgaa agtttccgga ctaaaccatt gagacatcag cggatatatc gccgggtaga
    19381 ggaaaatagc gacggtcccg aagataacaa cggttgcaac agccacggtt actttactgg
    19441 cttccgcttt cactaccggc tcagtcgcca gtaccgcggc agcaccacag atactgctac
    19501 cggcaccgat caaccagctg gtgtgcttat ccagaccaaa cactttctgc cccaggaagc
    19561 aagccagcag gaaggtactg gacagcgtca acacgtcaat gatgatccca ctgataccga
    19621 catcggcaat ttgcgagaac gtcagacgga agccataaag aatgataccc agacgtaata
    19681 aatattgctt ggcaaacagc acaccaccgt cacagctttt ccagatgtgc ggatagatgg
    19741 tgttgcctaa aaccatcccc aacaagattg cgagggtgag ggcactaaac ccggcacccg
    19801 caaccgcggg aatggaacca ccccacaggg cgaccccggt gataactgca ctcagggcta
    19861 accccggaat aaaatgccac agtgtacgat gttgtttctg taaggtgata ttcgtcataa
    19921 ccctctcctt taaccgggca taaggttacg actaactggt ttaaaaataa aattgattat
    19981 atatttataa ttaatcttta taagtggtaa gcgactatgc acatcaccct ccggcagttg
    20041 gaagtttttg cagaagtatt gaaaagtgga tcaaccaccc aggcgtcggt gatgctggcg
    20101 ttgtcgcaat cagcagtgag cgcagccttg accgacctgg aagggcagct tggcgtgcaa
    20161 ctgtttgatc gcgtggggaa aagactggtt gttaatgaac acgggcggct gctctatccg
    20221 cgtgcgttgg cattgcttga acaggcggtt gaaatcgaac aactgtttcg cgaagacaac
    20281 ggcgcgattc gtatctatgc cagtagtacc atcggtaact acattctgcc tgcagttatc
    20341 gcccgttatc gccatgatta tccgcagttg ccgattgaac ttagcgttgg gaacagccag
    20401 gacgtgatgc aagcggtgct ggatttccgc gttgatattg gctttattga aggaccgtgc
    20461 cacagcactg aaatcatttc tgaaccgtgg ctggaagacg agctggtggt tttcgccgcg
    20521 ccgacttcgc cgttggctcg tggtccggtc accttagaac agctggccgc tgcgccgtgg
    20581 atcctgcgtg aacgcggttc cggcacgcgg gagattgtcg attatctgtt gctgtcacat
    20641 ttaccgaagt ttgagatggc gatggaatta ggtaactccg aggcaatcaa acatgcggtg
    20701 cgtcatgggt tgggaattag ttgcctgtcg cgacgggtga ttaaagatca attgcaggca
    20761 ggcacattaa gtgaagttgc ggtccctctc ccgcgcctga tgcgtacgtt gtggcgtata
    20821 catcatcggc aaaaacacct ttccaacgcg ctacggcgct ttctggacta ttgcgatccc
    20881 gcgaatgtac cgcgttaagt tgctgtacaa gaacatgctg gtgctgtgtc gatttcgtga
    20941 cgcagcgccc tcagcatgca ttcgccagaa aagagattgg ctgctttact tataatccct
    21001 gggcgatcat gaaggtgtct tataaccgtg tatttctgcc ggaaggattg ccaatcgtct
    21061 gctacaatcg cgcctcattt ttaagatgga tagcattttt gtatggtttc cgaaactaaa
    21121 accacagaag cgccgggctt acgccgtgaa ttaaaggcgc gtcacctgac gatgattgcc
    21181 attggcggtt ccatcggtac aggtcttttt gttgcctctg gcgcaacgat ttctcaggca
    21241 ggtccgggcg gggcattgct ctcgtatatg ctgattggcc tgatggttta cttcctgatg
    21301 accagtctcg gtgaactggc tgcatatatg ccggtttccg gttcgtttgc cacttacggt
    21361 cagaactatg ttgaagaagg ctttggcttc gcgctgggct ggaactactg gtacaactgg
    21421 gcggtgacta tcgccgttga cctggttgca gctcagctgg tcatgagctg gtggttcccg
    21481 gatacaccgg gctggatctg gagtgcgttg ttcctcggcg ttatcttcct gctgaactac
    21541 atctcagttc gtggctttgg tgaagcggaa tactggttct cactgatcaa agtcacgaca
    21601 gttattgtct ttatcatcgt tggcgtgctg atgattatcg gtatcttcaa aggcgcgcag
    21661 cctgcgggct ggagcaactg gacaatcggc gaagcgccgt ttgctggtgg ttttgcggcg
    21721 atgatcggcg tagctatgat tgtcggcttc tctttccagg gaaccgagct gatcggtatt
    21781 gctgcaggcg agtccgaaga tccggcgaaa aacattccac gcgcggtacg tcaggtgttc
    21841 tggcgaatcc tgttgttcta tgtgttcgcg atcctgatta tcagcctgat tattccgtac
    21901 accgatccga gcctgctgcg taacgatgtt aaagacatca gcgttagtcc gttcaccctg
    21961 gtgttccagc acgcgggtct gctctctgcg gcggcggtga tgaacgcagt tattctgacg
    22021 gcggtgctgt cagcgggtaa ctccggtatg tatgcgtcta ctcgtatgct gtacaccctg
    22081 gcgtgtgacg gtaaagcgcc gcgcattttc gctaaactgt cgcgtggtgg cgtgccgcgt
    22141 aatgcgctgt atgcgacgac ggtgattgcc ggtctgtgct tcctgacctc catgtttggc
    22201 aaccagacgg tatacctgtg gctgctgaac acctccggga tgacgggttt tatcgcctgg
    22261 ctggggattg ccattagcca ctatcgcttc cgtcgcggtt acgtattgca gggacacgac
    22321 attaacgatc tgccgtaccg ttcaggtttc ttcccactgg ggccgatctt cgcattcatt
    22381 ctgtgtctga ttatcacttt gggccagaac tacgaagcgt tcctgaaaga taccattgac
    22441 tggggcggcg tagcggcaac gtatattggt atcccgctgt tcctgattat ttggttcggc
    22501 tacaagctga ttaaaggaac tcacttcgta cgctacagcg aaatgaagtt cccgcagaac
    22561 gataagaaat aagtttcctc ccttccttgc taagccctct caaccgagag ggctttttca
    22621 attccatttc cctgacaaat catgcggata taaaatttaa catttggatt gataattgtt
    22681 atcgtttgca ttatcgttac gccgcaatca aaaaaggctg acaaatcaga ggctgttccg
    22741 gctttctggg atgatcacct gcatacaaat aagtccaccg cgatgctgcc gtacgcaagg
    22801 ggacgtgaag aagatgtgag cgataaccca ttttattttc gtagttacct catggagata
    22861 tggaatgttt aggttgaacc ctttcgtacg ggtcgggctg tgtttgtccg ctatttcttg
    22921 tgcatggcct gtgttagcgg tcgatgatga tggcgaaacg atggttgtca ctgcatcttc
    22981 cgttgaacaa aacctcaaag atgcacccgc cagtatcagc gtcattaccc aggaagacct
    23041 gcagcgaaaa ccggtacaga atctgaagga tgtcctcaaa gaagtgcctg gcgtacaact
    23101 gacgaacgaa ggggataacc gtaagggcgt tagtattcgt ggtctggaca gcagctacac
    23161 cctgattctc gtcgacggta aacgcgttaa ctcccgcaat gccgtcttcc gccacaatga
    23221 tttcgatctg aactggatcc cggtcgattc catcgaacgt attgaagtgg ttcgtggccc
    23281 gatgtcgtcg ctatacggct ccgatgcgct cggcggtgta gtgaatatca tcaccaaaaa
    23341 aatcggtcag aaatggtcgg gtaccgttac cgtcgatacc accattcagg aacatcgcga
    23401 tcgcggtgat acctataacg gtcagttctt taccagtgga ccattaattg atggtgtgct
    23461 gggaatgaaa gcttacggca gcctggcaaa acgtgaaaag gatgacccgc aaaactcaac
    23521 gaccaccgat accggagaaa cgccgcgtat tgaaggattc tccagccgcg acggcaatgt
    23581 cgaatttgcc tggacaccga atcaaaatca cgattttact gccggatacg gtttcgaccg
    23641 tcaggatcgt gattccgact cgctggacaa aaaccgcctg gaacgccaga actactccgt
    23701 cagccataat gggcgttggg attacggcac cagcgaactg aaatactacg gtgagaaagt
    23761 cgagaacaaa aaccctggca acagcagccc gataacttcc gaaagcaata cggtcgacgg
    23821 caaatacacg ttgccgctga cggcaattaa tcagtttctc acggttggcg gtgaatggcg
    23881 tcacgacaaa cttagcgatg cggtgaacct gaccggggga accagctcca aaacgtctgc
    23941 cagccagtac gcgctgtttg tggaagatga atggcggatc ttcgagccgc tggcgctgac
    24001 gaccggcgtg cgtatggacg atcacgaaac ctacggtgaa cactggagtc cgcgtgccta
    24061 cctggtttat aacgccaccg acaccgtaac ggtgaaaggg ggctgggcga cggcatttaa
    24121 agcaccttct ctgttgcaac ttagccctga ctggacgagc aattcctgcc gtggcgcatg
    24181 taagattgtg ggtagcccgg atctgaaacc agaaaccagc gaaagttggg agctggggct
    24241 ttactacatg ggtgaagaag gctggctgga aggggttgaa tccagcgtta ccgttttccg
    24301 taacgatgtg aaagatcgta tcagcatcag ccgtacgtct gacgtcaacg ctgcaccggg
    24361 ctaccaaaac tttgttggtt ttgagacggg cgctaacgga cggcgcatac cggtatttag
    24421 ctactacaac gttaacaaag ctcgtattca gggcgtggaa accgaactga aaattccgtt
    24481 caacgatgaa tggaaactgt cgatcaacta cacctacaac gatggtcgtg atgtcagcaa
    24541 cggcgaaaac aaaccgctat ccgatctgcc gttccatact gctaacggta cgctggactg
    24601 gaaaccgctg gcgctggaag actggtcatt ctatgtttct ggtcactata ccgggcagaa
    24661 acgcgccgac agcgcgacgg ctaaaacacc gggcggttat accatctgga ataccggcgc
    24721 ggcctggcag gtgactaaag acgtcaaact gcgcgcaggc gtgctgaacc ttggcgacaa
    24781 ggatctcagt cgtgacgact acagctataa cgaagacgga cgtcgttact ttatggcagt
    24841 ggattatcgc ttctgatgag aagatgcccg gcgaaccggg cggactttca cttcagtaaa
    24901 tactgcgcat ggaagcgcag gtgatcctct ataaaagagg cgatgaagta gtaactgtga
    24961 tcatatcccg gctgaatacg cagcgtcatc ggccacgctt tctggcgcgc ggcttctgcc
    25021 agtaccgcag gttgcaactg gtcggcaaga aactgatcat tatcgccctg atcgataagc
    25081 gtcgggatcg catcctgcgc gttactggca tacatcagcg cgcaactgtc ccattccagc
    25141 catgcatttt tgtcctcacc taaatagctg ctaaacgctt tgattcccca cgggacgctg
    25201 cacggattca caattggcgc aaaggccgaa acgctggtgt atttacccgg atttttcagc
    25261 gccataatca gcgcaccgtg accacccatt gaatgaccgc taatggcgca gcggtcgctg
    25321 acattaaatt gcgactgaac cagcgccggt aattcatcgc gcagataatc atacatccgg
    25381 taatgcgtcg cccacggcgg ttgcgtggcg ttaagataaa accccgcgcc ctggcctaaa
    25441 tcgtagccat catcgttggc aacctgttcg ccgcgcgggc tggtgtctgg catcaccagt
    25501 acaatcccca gttctgccgc cacgcgctgg gcacccgcct tgatggtgaa gttctcgtca
    25561 ttgcaggtta atccggaaag ccagtacagc actggtggcg gggtgtgatc acgaggtggt
    25621 gggagaaaga tactgaacgt catcggacag tttaaggtac tggagtcgtg tcgccagcgt
    25681 tgctgccagc cttcaaaaca gcggtgctct tcgagcattt ccatgcgtgg ctccttgttg
    25741 tgttgtttgc aatttcttca tcataccgtt tattcaatgt gctgtgagta actttcactt
    25801 ccgtatttgc ataacgatgt tttaacatct gctgatgaaa ggcagcggca attacaataa
    25861 ttatcgctgt gaatactgga ttatgtgcgc cgcctcacgc acaataatca agctgtaaat
    25921 cagcttaata actttgcccc cacgcagggc ggaggcgtca cacctgcagg agaaatcata
    25981 aatgccatca ctcagtaaag aagcggccct ggttcatgaa gcgttagttg cgcgaggact
    26041 ggaaacaccg ctgcgcccgc ccgtgcatga aatggataac gaaacgcgca aaagccttat
    26101 tgctggtcat atgaccgaaa tcatgcagct gctgaatctc gacctggctg atgacagttt
    26161 gatggaaacg ccgcatcgca tcgctaaaat gtatgtcgat gaaattttct ccggtctgga
    26221 ttacgccaac ttcccgaaaa tcaccctcat tgaaaacaaa atgaaggtcg atgaaatggt
    26281 caccgtgcgc gatatcactc tgaccagtac ctgtgaacac cattttgtta ccatcgatgg
    26341 caaagcgacg gtggcttata tcccgaaaga ttcggtgatc ggtctgtcaa aaattaaccg
    26401 tatcgtgcag ttctttgccc agcgtccgca ggtgcaggaa cgtctgacgc agcaaattct
    26461 tattgcgcta caaacgctgc tgggcaccaa taacgtggct gtctcgatcg acgcggtgca
    26521 ttactgcgtg aaggcgcgtg gcatccgcga tgcaaccagt gccacaacaa cgacctctct
    26581 tggtggattg ttcaaatcca gtcagaatac gcgccacgag tttctgcgcg ctgtgcgtca
    26641 tcacaactga ttaaaaggca ggaaccatgg agcgcaacgt cacgctcgat tttgttcgcg
    26701 gcgtcgccat tctggggatc ctgctattaa acatcagcgc ctttgggcta ccaaaggcgg
    26761 cttatctcaa tcccgcctgg tacggtgcta ttacgccgca ggatgcatgg acctgggcat
    26821 ttcttgatct catcgcccag gtgaaattcc tcacgctttt tgcgctgctg tttggtgctg
    26881 gactgcaaat gttgctgccc cgtggcagac gctggatcca gtcgcggtta acgctgttag
    26941 tcttattggg ctttattcac ggtttattgt tctgggacgg cgatattctg ctggcttacg
    27001 ggctggtggg cttaatctgc tggcggctgg tgcgcgatgc gccatcggta aaaagcctgt
    27061 ttaatacagg cgtcatgctt tatctggtgg ggcttggcgt tttgctgtta ttggggctga
    27121 tttccgacag ccagaccagc cgcgcctgga cgccggatgc atcggctatt ttgtatgaaa
    27181 aatactggaa gcttcacggc ggcgttgaag cgatcagtaa tcgtgccgat ggtgttggca
    27241 acagtttact ggcactgggc gcacagtatg gctggcaact ggctgggatg atgctcattg
    27301 gtgcagcgct gatgcgcagc ggctggctga aagggcagtt cagcttacgt cactatcgtc
    27361 gtactggttt tgtgctggtg gcgattgggg tgaccattaa ccttcctgcc atcgccctgc
    27421 aatggcagct ggactgggcg tatcgctggt gtgccttctt acttcaaatg ccgcgggaac
    27481 tgagtgcgcc gtttcaggcg attggctatg cgtcgctgtt ttatggcttc tggccgcaat
    27541 tgagccgctt taagctggtg cttgcgatcg cctgcgtcgg acggatggcg ctgaccaact
    27601 atctattgca aacgctgatt tgtaccatgc ttttttacca cctcggtttg tttatgcatt
    27661 ttgaccgcct ggagctgctg gcgtttgtta ttccggtatg gctggcgaat atcctcttct
    27721 ctgttatctg gctgcgttac ttccgccagg ggccggtgga atggctctgg cgtcagttaa
    27781 ctttgcgtgc tgccggaacg gcaatatcta aaacatcaag ataacgatct ggatcacatt
    27841 cgttaacaaa acggctgtaa ccgtttccat tgctgtgact cgattcacga agtcctgtat
    27901 tcagtgctga caaaatagcc gccagcaagc agtcatttac tgcaatctca taacaggtag
    27961 tgaatatgat caccattcgt gatgtagcgc gtcaggctgg cgtctctgtg gcaacggttt
    28021 cccgggtgct caataacagc acgctggtca gtgccgacac gcgtgaagca gtaatgaaag
    28081 ccgtgagtga gctggattat cggccaaacg ccaatgccca ggcgctggca actcaggtta
    28141 gcgacaccat tggcgtggtg gtgatggacg tttctgatgc gtttttcggc gcgctggtaa
    28201 aagcggtgga tctggtcgct cagcagcatc agaaatacgt gctaatcggc aatagctatc
    28261 atgaagcgga aaaagagcgt cacgccattg aggtgttaat tcgccagcgt tgtaatgcgt
    28321 tgattgttca ctcaaaagca ttgagtgacg atgaactggc gcaatttatg gataacattc
    28381 ccggtatggt gttaatcaac cgcgttgtgc cggggtacgc ccatcgttgc gtttgcctgg
    28441 ataatctcag cggtgcccga atggcgacgc gcatgttgct gaataacggt catcaacgta
    28501 ttggttatct ttcttccagt cacggcattg aagatgacgc catgcgtaaa gcaggctgga
    28561 tgagtgcgtt gaaagagcag gatattattc cgccggaaag ctggattggc actggtacgc
    28621 cggacatgcc gggcggtgag gcggcgatgg ttgaactgct ggggcgcaat ctacaactta
    28681 ccgctgtatt tgcttataac gacaatatgg ctgctggcgc actgacagca ttaaaagata
    28741 atggcattgc gattccgtta catctctcaa tcatcggttt cgatgatatt cccatcgccc
    28801 gttacaccga cccgcaatta acgaccgtgc gttatcccat tgcttcaatg gcgaaattag
    28861 ccaccgaact ggccttgcag ggggcagcag gcaatattga tcctcgtgct agccactgtt
    28921 ttatgccgac gttagtgcgt cgccattctg tcgcaacacg ccagaatgcg gcgacgatca
    28981 ctaactcaac aaatcaggcg atgtaaccgc tttcaatctg tgagtgattt cacagtatct
    29041 taacaatgtg atagctatga ttgcaccgtt ttaacgttgt aacccgtatg taacagtgaa
    29101 taatcacttt caccgaggta atagcgtcat aacaacaatt aaagccgttt tctggagcgt
    29161 taccgggcat ggaagaacga attttaaaaa gtgagcttcg gcgttcagta acacttcatt
    29221 aactctactg ccccgccgag catttatctc aagcactacc ctgcataaga aaaaccggag
    29281 ataccatgaa taagaaggtg ttaaccctgt ctgctgtgat ggccagcatg ttattcggtg
    29341 ccgctgcaca cgctgctgat actcgcattg gtgtaacaat ctataagtac gacgataact
    29401 ttatgtctgt agtgcgcaag gctattgagc aagatgcgaa agccgcgcca gatgttcagc
    29461 tgctgatgaa tgattctcag aatgaccagt ccaagcagaa cgatcagatc gacgtattgc
    29521 tggcgaaagg ggtgaaggca ctggcaatca acctggttga cccggcagct gcgggtacgg
    29581 tgattgagaa agcgcgtggg caaaacgtgc cggtggtttt cttcaacaaa gaaccgtctc
    29641 gtaaggcgct ggatagctac gacaaagcct actacgttgg cactgattcc aaagagtccg
    29701 gcattattca gggcgatttg attgctaaac actgggcggc gaatcagggt tgggatctga
    29761 acaaagacgg tcagattcaa ttcgtattgc tgaaaggtga accgggccat ccggatgcag
    29821 aagcacgtac cacttacgtg attaaagagc tgaacgacaa aggcattaaa actgaacagt
    29881 tacagttaga taccgctatg tgggataccg ctcaggcgaa agataagatg gacgcctggc
    29941 tgtctggccc gaacgccaac aaaatcgaag tggttatcgc caacaacgat gcgatggcaa
    30001 tgggcgcggt agaagcactg aaagcacaca acaagtccag cattccggtg tttggcgtcg
    30061 atgcgctgcc agaagcgctg gcgctggtga aatccggtgc actggcgggc acagtactga
    30121 acgatgctaa caaccaggcg aaagcgacct ttgatctggc gaaaaacctg gccgatggca
    30181 aaggtgcggc tgatggcacc aactggaaaa tcgacaacaa agtggtccgc gtaccttatg
    30241 ttggcgtaga taaagacaac ctggctgaat tcagcaagaa ataagactga ttgggcgcag
    30301 tcgtttactg cgcccttatt gtatggccaa caaggtaaaa ttatatggtc agctcaacga
    30361 ctccgtcttc cggggaatac ttgttggaaa tgagcggtat caacaagtcc tttcctggtg
    30421 ttaaggcact tgataacgtt aatttaaaag tccggccaca ttctatccat gcattaatgg
    30481 gggaaaacgg tgcaggaaaa tcgacattat taaaatgcct gtttggtatt tatcaaaaag
    30541 actccggcac cattttattc cagggtaaag agatcgattt ccattccgcc aaagaggcac
    30601 tggaaaatgg tatttcgatg gtacaccagg agttaaacct ggtattacaa cgttcggtga
    30661 tggataacat gtggctgggg cgatatccca ccaaaggcat gtttgtcgat caggacaaaa
    30721 tgtaccgcga aaccaaagcg atttttgatg aactggatat tgatatcgat ccgcgtgcgc
    30781 gcgtcggcac attatccgtt tcgcaaatgc agatgatcga aatcgccaaa gcgttttcct
    30841 ataacgcgaa aattgtgatt atggatgaac cgacttcttc gttaaccgaa aaagaggtca
    30901 atcatctgtt cactattatt cgtaaattaa aagagcgcgg ctgcggtatt gtttatatct
    30961 cgcataaaat ggaagaaatc ttccagttat gtgatgaagt taccgtattg cgcgacggtc
    31021 agtggatcgc caccgaaccg ctggcaggac tgacgatgga caagatcatc gccatgatgg
    31081 ttgggcgttc tcttaatcag cgttttcctg acaaagaaaa caagccgggc gaagtcatcc
    31141 tcgaggtgcg taacctaacg tcactgcgcc agccgtcgat tcgcgatgtc tcgtttgatc
    31201 tgcataaagg ggagatcctc ggtattgccg ggctggtggg ggcgaaacgt accgatattg
    31261 ttgagacgtt atttggtatt cgcgagaaat cggctggcac cattacgttg cacggcaaac
    31321 agatcaataa ccataatgcc aacgaagcca taaaccacgg atttgcactg gtaactgagg
    31381 agcgccgctc aacgggaatt tatgcctatc tggatattag ttttaactcg ttaatttcca
    31441 atattcgcaa ctacaaaaat aaagttggtt tactggataa ctcgcggatg aaaagcgata
    31501 cccagtgggt gattgattcg atgcgggtaa aaacgccggg tcatcggacg caaattggtt
    31561 cgctctccgg tggtaatcag caaaaggtga ttattggtcg ctggctacta acgcaaccag
    31621 aaatattaat gctcgatgaa ccgacgcgcg gtattgatgt cggggcgaag tttgaaattt
    31681 atcagttaat tgccgaactg gcgaagaaag gcaaggggat tattattatc tcctctgaaa
    31741 tgcctgagtt gttagggata acagaccgta ttctggtcat gagcaatggt ctcgtttccg
    31801 gaattgtcga tacaaaaaca acaacgcaaa acgaaattct gcgtcttgcg tctttgcacc
    31861 tttaagatta ggggcttccc atgagtgcgt taaataagaa aagttttctt acttacctga
    31921 aagagggcgg tatttacgtc gttcttttag ttttgctggc gattattatt ttccaggacc
    31981 caacattttt aagtctgttg aacttaagta atattctcac ccagtcatcg gtgcgtatta
    32041 ttatcgcgct cggtgtggca gggttaattg tcacccaggg gaccgatctt tctgctggtc
    32101 gtcaggtagg gctggcggca gtggtggctg cgacattatt gcagtccatg gataacgcca
    32161 acaaagtgtt cccggaaatg gcgacgatgc cgattgcgct ggttattctg attgtctgtg
    32221 ccattggtgc ggtgatcggt ttgatcaacg gtctgattat cgcttatctc aacgtgacgc
    32281 cgttcattac cacgctcggc acgatgatca tcgtctatgg aatcaactcg ctctattacg
    32341 actttgtcgg ggcgtcgcca atttctggtt ttgacagtgg cttctctacc tttgctcagg
    32401 gctttgtcgc gctggggagt ttccgtctct cttacatcac cttctacgcg ttgattgcgg
    32461 tggcgttcgt ctgggtgttg tggaacaaaa cccgcttcgg taagaacatt tttgccattg
    32521 gcggtaaccc ggaagcggca aaagtatctg gtgtcaacgt cggcctgaac ctgctgatga
    32581 tctacgcgtt gtctggcgtg ttctatgcct ttggcgggat gttagaagcc ggacgtatcg
    32641 gctctgccac caacaacctc ggctttatgt atgagctgga tgctatcgcg gcgtgcgtgg
    32701 taggcggtgt atcgttcagc ggcggtgtgg ggacggtgat tggcgtggtg accggggtaa
    32761 ttatttttac cgtcatcaac tatggcctga cgtatatcgg cgtaaaccca tactggcagt
    32821 acatcatcaa aggggcgatt attatcttcg ccgtagcgct ggattcactg aaatacgcgc
    32881 gtaagaagtg atgtgattgt ggatattgcc ggatgcgctg catccggcaa tcggtgccaa
    32941 atgccggatg cgtctctggt tttacaacgt taccgggtgt tctttctcgc ctttcttaaa
    33001 cttcacttcc ccgagctcaa tacaacccac cgggcagacg tgaccacaaa gcagacaacc
    33061 cacacatttc tcggtattac aatgcggtgt gcgggttttc tcgctccatt ccatcgcctg
    33121 gtgaccgccg tcgtaacagg aaatataaca gcgtccacag ccaacacatt tatcaagatt
    33181 gatacgggga tagacaatat aactgcggtc taaatcttcc gccgggacaa tattgttatt
    33241 cgccagacct accatctcct gcagcgaatc aaatccctga tcggcgagat aatgcgacaa
    33301 cccgcttgcc atatcttcca ctatccgata cccgtactgc atgatgccgg tggtcacctg
    33361 taacgttgct gcgccgagca ataaaaactc agccgcatcc tcccaggttt caatgccgcc
    33421 gataccgctg attgggaaat cgcgcagttc tggatgggtg cgcatctgct gaatgaagcg
    33481 cagggcgatc ggttttaccg ctttaccgga atatccggaa atactcgatt ttccgttaac
    33541 gatcggcata ccgattttct gattaagatc gatattggtg atggatttaa cggtgttaat
    33601 cgccgcaatg ccatctgcgc cgccgcgctt cgccgccagc gccacttcgc acatatcacc
    33661 gatattcggc gtcatcttcg ccagcattgg cagcgtggaa ccccgtttca ctgcccgaca
    33721 atatttttct accagctccg ggctttgccc gacatcgcta cccatcgcat gagaagtcat
    33781 ttgcggacag gagaagttac actcgatcat atccgcgcca gcttcttgca ccaggcgcgc
    33841 cagctcctcc cattgctgct cattttcccc catgatcgaa gcgatcaata ctttgtccgg
    33901 gtaatcttcc ttcagctgac gcagggcggc cagattctct tccaacggat gttcagcaat
    33961 ctgctccata tttttgaagc cgataaaacc ggtatcttct ttcaccagat gatcaaaacg
    34021 cggcgagact tcgttggcga taaaaaagcc gatcgtttta aacaccacgc cgccccaacc
    34081 tgtgtcgtag gctttggcac acatctcata gcagttgcct acaggcgaag aagagaggca
    34141 gaacgggttg ggaaacttca cgccgcaaaa agtaatcgaa agatctttcg ttaacatgag
    34201 caagctccct ctaaatagtg atgaatcgcc tcggcggctt ctttcccggt tttcacggca
    34261 tagaccacgg ttttgtcacc ctcaacaata tcgccagcag caaagacttg cggatcgcgg
    34321 gtctgataat tttgtgtttt gatggtgtta cgctgcggct ctaactccgc aaaggcatcc
    34381 agtctggcat gctgaccgac ggcgagaata attttatctg ccgccatcgt cagttcgccc
    34441 ggtaaccgta catgcttaaa cgtgacttta ttgccttcca cggctactgg cgtgaatcca
    34501 tcgatgatcg aaacgcccag ctcccgggcg ctggcaaact ctttttcgct tgccggaaac
    34561 tcatctaact cttcacgcgc tacgcaggtt accgcctgac agccgagaac tttcagcgtg
    34621 ctggctacgt ccatcgcaac atcaccgccg ccgataatta atgcgctttg tggaatgctg
    34681 atatcgcctt gcgcctgtcg tgcacgttgc aggaagtcga cggcaatctc aacgtcgcta
    34741 tgctcaaaca gcgataaccc ggaaccgctc gataacccca cggtgaccag taccgcgcgg
    34801 ttttctgctt tcagctgctc aagggtgagt gtgttaccga cttcgttgtt gcacttaatg
    34861 gtcacgccca ttttttcaat acgggcgatc tctgcatcca gcactgattg cggtaaccgg
    34921 aattgcggaa taccgttacg caaccagcca ccggggtgcg cttctttctc ataaatcgtg
    34981 acgtcataac cctggtttgt cagtgttaca ctggcctgca atccggcagg acctgcgcca
    35041 ataatcgcga ctttgccgag cgttttagta ccgggctgat aaatttccat cccggtttgt
    35101 tgttcaaaat cagtaacaaa acgctgtaag cgaccgatat caatgggagt atcaacaccg
    35161 gcacgggtac aaccgctttg acataatttc tccgtcgggc acactctggc acaaacggca
    35221 ccgagggcat tattttcgcg aattgtctcg gcagcgcctt taaaattacg aaagtagatt
    35281 gagcgaataa atttccccgg atcggtctgt gccgggcaag cctgactaca gggagcgtca
    35341 tgacataata aacagcgaga ggcttcttta atcgccagta aaggcgtaaa agccggagtg
    35401 agttcatcca gataattttg ttgcggcatg tgatcttcct taatgctatg agtaattaag
    35461 gcgaacgcta aatatactcg tcatacttca agttgcatgt gctgcgtctg cgttcgctca
    35521 ccccagtcac ttacttatgt aagctcctgg ggactcactc acttgtcgcc ttcctgcaac
    35581 tcgaattatt tagagtataa atattgcgtg ctgatattaa ggctatgcgc gtggggaaat
    35641 ttatactgtg aaaggattca caaatagctg cggttcagaa tgttatatcc gcagcttatt
    35701 gagtgaagta ataattattc tcaataatta acagaaaaga gaaaatatta acgatagaaa
    35761 ataagataaa caatcatgcc gatagataaa attccgaagc cgatatatgc caccatataa
    35821 gcgaaaaaga gtccgggatt ttttgctcgc cagacataca ccggttccgg tgcattttta
    35881 acccgtttat caatcgcgcc tgcgcgccag cctttccatg cttcgcggaa acaaaatatc
    35941 ggaataagga aaaaaacggc aacgacaaaa aactgctgta catccataac tactttcctt
    36001 gtttcttttg taattcaagt aactgttcgg gtgtgacggc gggatacccc tgcgcatctt
    36061 ccggcacctg gtgcatagcc ggaataggga ccagcggccc taaaaaacgc ggttcacgtt
    36121 taaaaatata aaggtcagcc agcgcaccga aacgggcggc aaattcgcga atacgtactg
    36181 acagcatatc tttcggtgac ggtacggcat aacactgagc ctgaatcccc atatgcagcg
    36241 caataaataa tgcccgctca cagtggaaac gttgggtgat aataatgaaa tcattagtat
    36301 cgaaaacttt gcgtgtacgc acgatggaat ccagcgtacg aaagcctgcg taatcgagaa
    36361 caatatctga tgggtcgaca ccagcagcga ttaaatcttt gcgcatggtc atcggctcat
    36421 tataactttg caatgcgtta tcgccgctca gtaatagata atttacctta ccgctgttat
    36481 aggcattaat cgctccttga atgcggtagc gataatactg attaattacg ccagtacgat
    36541 aatattttgc tgttccgagc accacaccga cctggcggta ggggagatcc tgcaattcgt
    36601 cgtagatata aggcgcggtt ttccagctca tccagcgatc gaggccgagc acagtcaaca
    36661 gcagcaagcc gatcaggact aacaggctga ggaacacgcg ctttaacatg caggagactc
    36721 gatctaagga aaatttccat caggctactt tacccgctgg ttttgcgcaa gaaacggcaa
    36781 actgtttgcc gggaatttcg acaccaggag agggctggca agccggacaa ggcgttcacg
    36841 ccgcatccgg caccaggctt aagcgagaag cactcggtcg atactgtgac agccgagagc
    36901 tttcagcgtt gcggaggtgg catcccactg aatcaacggc gcatcggctt tttctgccag
    36961 aaccgcgcgc tggatatccg ggtaatcata acccttgaga ttcaacagaa ttaacgctcc
    37021 ctgcaacggt ggcagagtcg ggttgaatgc ggcgttttca gcgtagctgc cactgaaaat
    37081 acgaccgtct ttacattcca gcgcgacacc gcttggcgac ttactgtaag gcatgtgcga
    37141 acggtttgcc gctgcaatcg ccgcctgaga aagcgcatca ccagtcagcg catagccgtg
    37201 atcctgctcg tccatcagca gcgttttaat ctccagatct ttcggcccaa aggcatccgg
    37261 cagatagtca cgcagcgcgt gtgcctcgcg gcccggcaga tgaatacgca gatccagacc
    37321 gctgttcagt tcattcataa actgacggca gtgaccacaa ggcgtgtagt taacggtgat
    37381 ggctgcaagc gctttttcac cactcaacca ggcgtggctg atcgcgcttt gttcggcatg
    37441 aacggtttgc tgcattgtcg caccaataaa ttccatattg gcaccgaaat accaggttcc
    37501 gctcacaccg cgcgcaattg cgccaacatt aaaattcgac aatggcgtac gcgcacaggc
    37561 cgccgccagc ggaagtagtg cgaatgccag cgcgtcttcg tccagccccg ttgcgctctt
    37621 cagcgatgag acttgctccc cggtcaacaa agcggggaag tacttgtctg ccagaatagg
    37681 ttccagtgca gattgcaagt tatccgcaag ttgggcaaaa gcggtttgaa aacgtggatg
    37741 catggcgttg cctcataatc atgtaatggg ataacagttt acgaacccgt tatggcttta
    37801 tatgtgatct gaatctcatt aattatgcaa cgcgtgaaac ttacatcaaa aatgcgcgac
    37861 gcatcgcaaa ttttagccca ttactgccag aataatcggg aaaaggaacg gtgcgatcag
    37921 cgaagtaatt atcccgcata acaccagcgc tagcgaacta aatgcacctt cctgataatc
    37981 cagctcggcg cagcgcgccg taccgagggc gtgcgaggca gtccccattg ccagaccgcg
    38041 cgcagctttg gtacgaatac gcatcgcatt aagcaatgta tggccaaata ccgcgccgag
    38101 gatgccgacg aaaatcacgc aaactgcgct gattgccgga ataccgccaa tactgccgcc
    38161 aaccgccatt gcaataggcg tggtgactga tttcggcagg attgacgcgg cgatttccgg
    38221 tgaagcgccc atcaacaatg ccacggaagt ccccgtcacc atcgcaacca cgctgccgat
    38281 aaaacagatg gtgatgatcg atttccagcg cgcgcggatc tggtgtagct gctcatataa
    38341 aggataggcc agcgcgacga ccgccggttg cagcagatcg tttagcactt cgctaccttt
    38401 aaagtagctg tcgtaagaga tgccggtcag cattaaaaaa ggaatgatca ccaccattgc
    38461 taccagcaac gggttaagca ggggaaactt ataccgtgcc gccagtttgc gggcggcaaa
    38521 aaagacaatc aacgtcagcg gtaatgacca ccagatattt gccatcattc ttctgatcct
    38581 ttctgaccta cgactttacg ttcaccgtgt accagttgcg aactccagct caccaccaga
    38641 aaaaccacca gcgtactgat tgcacaggaa accactaccg ggccaaactg tgcgcggagc
    38701 aaatcaaaat attgcatgac gcctacgcca atcggcacaa acaatagcgc catatagcga
    38761 atcagtacgt agcaccccgg attgacccat tttgccggaa gaatttgcaa ggccagcagg
    38821 acaaacagga tcagcatacc gatgatgctg cccggaatgg ttaccggtag cagggaagca
    38881 atgaaaatgc ctgcatacag gcaggcataa atcaggacga aagcgcgtaa atattgccag
    38941 ataatgttca gtgtcttact cataatcaac accccagatg agatggattc atcatacaat
    39001 taattcgtaa aacgtgctac cgatcacatt aagaaatcag cgcttatttt ttcgctcccc
    39061 ggcattacgc aatgcgcaac gaactcaggc atacatcaag catagagagg gattacagtt
    39121 tgcagcaatc cctctttgca gacctttaat ccgttgacca taggcgctaa catagcgcct
    39181 catttttttg cgggtgaaga tatgcgtgtg ttactggctc cgatggaggg tgtgcttgac
    39241 tctctggtgc gtgaattgct gaccgaagtt aacgactacg atctgtgcat caccgagttt
    39301 gtccgcgtgg tggatcaact gctgccggta aaagtttttc atcgcatttg ccctgagcta
    39361 caaaacgcca gccggacacc atccggtacg ctggtgcgcg tgcagttgtt gggtcagttc
    39421 ccacaatggc tggcagagaa cgccgcccgt gcggtggaat taggttcctg gggcgtggat
    39481 ctcaattgcg gctgcccgtc gaaaacggtc aacggtagcg gtggtggggc gacgttactc
    39541 aaagatcctg aactcatcta ccagggtgca aaagcgatgc gtgaagctgt accggcgcat
    39601 ttgcccgtca gcgtgaaagt gcgtctgggc tgggacagcg gtgagaagaa atttgaaatc
    39661 gccgatgcgg ttcaacaggc tggtgctacg gagctggtgg tgcatggacg gacaaaagag
    39721 cagggttacc gcgcggagca tattgactgg caggcgattg gcgagattcg ccagcggctg
    39781 aatattccgg tgattgccaa cggcgaaatc tgggactggc agagcgcgca acaatgcatt
    39841 gcgatcagcg gctgcgacgc agtgatgatt ggtcgcgggg cgctcaatat tcccaacctg
    39901 agccgggtag taaaatataa cgaaccgcga atgccgtggc cggaggtggt tgctttgctg
    39961 caaaaatata cccgtctgga aaagcagggc gataccgggt tatatcacgt tgcgcggatt
    40021 aaacagtggt tgagttattt gcgtaaagaa tacgatgaag caacggaatt atttcagcat
    40081 gttcgggtgt tgaataattc ccctgatatt gcaagggcta ttcaggcaat tgatatcgat
    40141 aagctgcgat aatagtggaa tcgcagttta tcagagacaa tgaatcgctt attcagcttc
    40201 atcatcttgt tgcgctcctt tcatgagcta
//
LOCUS       DAZGLJ010000020        39620 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000020 DAZGLJ010000000
VERSION     DAZGLJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.020"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            87..941
                     /locus_tag="ACHK49_000952"
     CDS             87..941
                     /locus_tag="ACHK49_000952"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HFO9261639.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            977..1867
                     /locus_tag="ACHK49_000953"
     CDS             977..1867
                     /locus_tag="ACHK49_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO9261640.1"
                     /translation="MKNIKLLGSAMLVSLALFSQNSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLNGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            1928..3427
                     /locus_tag="ACHK49_000954"
     CDS             1928..3427
                     /locus_tag="ACHK49_000954"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO9261641.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPDENVPAKLEVKNLSHPSLFKEI
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            3428..4417
                     /locus_tag="ACHK49_000955"
     CDS             3428..4417
                     /locus_tag="ACHK49_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9261642.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFILLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGI
                     NVTRLKIKVFMITAVLAGISGMILASRRYSGQPNSALSYELDAIAAAVLGGTSLNGGY
                     GTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            4439..5401
                     /locus_tag="ACHK49_000956"
     CDS             4439..5401
                     /locus_tag="ACHK49_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO9261643.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            5398..6444
                     /locus_tag="ACHK49_000957"
     CDS             5398..6444
                     /locus_tag="ACHK49_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO9261644.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGIVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDGIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVLRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSVTPRTP
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEMSALKAEIAGYR"
     gene            complement(6536..7885)
                     /gene="accC"
                     /locus_tag="ACHK49_000958"
     CDS             complement(6536..7885)
                     /gene="accC"
                     /locus_tag="ACHK49_000958"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO9261645.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7896..8366)
                     /gene="accB"
                     /locus_tag="ACHK49_000959"
     CDS             complement(7896..8366)
                     /gene="accB"
                     /locus_tag="ACHK49_000959"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO9261646.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPIMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9232..10062)
                     /locus_tag="ACHK49_000960"
     CDS             complement(9232..10062)
                     /locus_tag="ACHK49_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9261647.1"
                     /translation="MTSLFNRLTGKAVSRTAFVEHLGQEVVQHHPNWKVMISTDHKLM
                     RIDTGHQTVEITLDDAYKRYQAAPDPLQEVAADMLKQLAEMLPTGRCIVPVIKEKAWL
                     DTVRQQLSQYESDPEVLNASLDFAWEDFNDELIILFAEDGSETMRYLMRYEVTIDREQ
                     AISNLKQILPEVTAEIVDTGANDGKVAVISAGGTYEPSLMLIDDLWVQSPDPECGDLL
                     VTVPSRGKLFFTHNDQPANLSALRQLTKQCFREDDHPLTTQIFIRRNGHFYPWVETLH
                     "
     gene            complement(10099..11073)
                     /locus_tag="ACHK49_000961"
     CDS             complement(10099..11073)
                     /locus_tag="ACHK49_000961"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO9261648.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            11225..13165
                     /gene="csrD"
                     /locus_tag="ACHK49_000962"
     CDS             11225..13165
                     /gene="csrD"
                     /locus_tag="ACHK49_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO9261649.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13470..14513
                     /gene="mreB"
                     /locus_tag="ACHK49_000963"
     CDS             13470..14513
                     /gene="mreB"
                     /locus_tag="ACHK49_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO9261650.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            14579..15682
                     /gene="mreC"
                     /locus_tag="ACHK49_000964"
     CDS             14579..15682
                     /gene="mreC"
                     /locus_tag="ACHK49_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO9261651.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            15682..16170
                     /gene="mreD"
                     /locus_tag="ACHK49_000965"
     CDS             15682..16170
                     /gene="mreD"
                     /locus_tag="ACHK49_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO9261652.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            16179..16772
                     /gene="yhdE"
                     /locus_tag="ACHK49_000966"
     CDS             16179..16772
                     /gene="yhdE"
                     /locus_tag="ACHK49_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO9261653.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            16762..18231
                     /gene="rng"
                     /locus_tag="ACHK49_000967"
     CDS             16762..18231
                     /gene="rng"
                     /locus_tag="ACHK49_000967"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO9261654.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            18299..22099
                     /gene="yhdP"
                     /locus_tag="ACHK49_000968"
     CDS             18299..22099
                     /gene="yhdP"
                     /locus_tag="ACHK49_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO9261655.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTSVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            22255..23700
                     /gene="tldD"
                     /locus_tag="ACHK49_000969"
     CDS             22255..23700
                     /gene="tldD"
                     /locus_tag="ACHK49_000969"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO9261656.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNCR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(23828..24757)
                     /gene="aaeR"
                     /locus_tag="ACHK49_000970"
     CDS             complement(23828..24757)
                     /gene="aaeR"
                     /locus_tag="ACHK49_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO9261657.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            24940..25143
                     /gene="aaeX"
                     /locus_tag="ACHK49_000971"
     CDS             24940..25143
                     /gene="aaeX"
                     /locus_tag="ACHK49_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO9261658.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            25151..26083
                     /gene="aaeA"
                     /locus_tag="ACHK49_000972"
     CDS             25151..26083
                     /gene="aaeA"
                     /locus_tag="ACHK49_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO9261659.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            26089..28056
                     /gene="aaeB"
                     /locus_tag="ACHK49_000973"
     CDS             26089..28056
                     /gene="aaeB"
                     /locus_tag="ACHK49_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO9261660.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     SMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            28148..28420
                     /locus_tag="ACHK49_000974"
     CDS             28148..28420
                     /locus_tag="ACHK49_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO9261661.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(28476..28739)
                     /gene="yhcN"
                     /locus_tag="ACHK49_000975"
     CDS             complement(28476..28739)
                     /gene="yhcN"
                     /locus_tag="ACHK49_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO9261662.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(29104..29574)
                     /gene="argR"
                     /locus_tag="ACHK49_000976"
     CDS             complement(29104..29574)
                     /gene="argR"
                     /locus_tag="ACHK49_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO9261663.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            30009..30947
                     /gene="mdh"
                     /locus_tag="ACHK49_000977"
     CDS             30009..30947
                     /gene="mdh"
                     /locus_tag="ACHK49_000977"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO9261664.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            complement(31010..32077)
                     /gene="degS"
                     /locus_tag="ACHK49_000978"
     CDS             complement(31010..32077)
                     /gene="degS"
                     /locus_tag="ACHK49_000978"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO9261665.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(32167..33534)
                     /gene="degQ"
                     /locus_tag="ACHK49_000979"
     CDS             complement(32167..33534)
                     /gene="degQ"
                     /locus_tag="ACHK49_000979"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO9261666.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(33688..34086)
                     /gene="zapG"
                     /locus_tag="ACHK49_000980"
     CDS             complement(33688..34086)
                     /gene="zapG"
                     /locus_tag="ACHK49_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO9261667.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            34280..35407
                     /gene="zapE"
                     /locus_tag="ACHK49_000981"
     CDS             34280..35407
                     /gene="zapE"
                     /locus_tag="ACHK49_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO9261668.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            35626..36054
                     /gene="rplM"
                     /locus_tag="ACHK49_000982"
     CDS             35626..36054
                     /gene="rplM"
                     /locus_tag="ACHK49_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO9261669.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            36070..36462
                     /gene="rpsI"
                     /locus_tag="ACHK49_000983"
     CDS             36070..36462
                     /gene="rpsI"
                     /locus_tag="ACHK49_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO9261670.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            36857..37495
                     /gene="sspA"
                     /locus_tag="ACHK49_000984"
     CDS             36857..37495
                     /gene="sspA"
                     /locus_tag="ACHK49_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO9261671.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            37501..37998
                     /gene="sspB"
                     /locus_tag="ACHK49_000985"
     CDS             37501..37998
                     /gene="sspB"
                     /locus_tag="ACHK49_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO9261672.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(38041..39408)
                     /gene="dcuC"
                     /locus_tag="ACHK49_000986"
     CDS             complement(38041..39408)
                     /gene="dcuC"
                     /locus_tag="ACHK49_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO9261673.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVAV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9704 a         9528 c        10455 g         9933 t
ORIGIN      
        1 tttgctgtgc tagtttgctt tcaaaatgaa agtgaatatt ctctttcttc attttgtaag
       61 gcgaacgaca aataacagga gctgcgatgt acatcatttc aagcaaaaac atgctgaaaa
      121 aagcccagtc agagggatat gccgtccccg ctttcaacat tcataacctt gaaactttac
      181 aggtagttgt tgagagcgct gcacaacttc gctctcccgt tatgctggcg ggaacacccg
      241 gtacttatcg ttatggtggc gtgggaagtt taatttcgtt agtacaatcg ctggctcgcg
      301 agtacaacct gccgttagtc ctgcatctcg accatcatga agagagtgat gacattttta
      361 acaaggttcg cgcagggatc cgttctgtca tgattgatgg ctcccatttg ccatttgaag
      421 acaacattgc gcgagtgcaa gaagttacca ccttttgcca tcgctttgat gttagcgttg
      481 aggccgagct agggcgtctt ggcggccagg aggacgatct gctggtggat gaaaaagatt
      541 ccgcttatac ctcaccagcc gcagctgcag agtttgttca acgtacccaa attgactccc
      601 tggccgtagc aatcggcaca gcacatggtt tatatgcgca tgaaccacga ctggattttg
      661 tgcggctgga acagattcgt cagcgagtgg agattccact ggtgttgcat ggtgcttcgg
      721 gcttatcaga acaggacgtt cagcactgta ttcgccgcgg tatttgtaaa gtgaatgtcg
      781 cgacagaatt gaaaattgcg tttgccgatg cgttaaaagg atatttctac aacaatcctg
      841 cggcaaatga tccacgccac tatatgcagc ccgcaaaggc cgccatgaaa gaagtcgtaa
      901 ctcgtattat tggtgtctgt ggtagtgaag gaaaaattta agacgttgta ccctacaaaa
      961 acaatgagga ttgtttatga aaaatattaa attacttggt tcagctatgt tggtgagcct
     1021 ggcattgttc tcgcaaaatt cgttggcaaa agagtataaa attggtgcgt cattattaac
     1081 acagcaacat cctttctata ttgatcttgc gaatgccatg aaagaagagg caaaaaaaga
     1141 caatgtcaat ttaagtgtct ctattgccaa tcaggattta aacaaacaac tttctgatgt
     1201 tgaggatttt attactaaaa aagtggatgc cattattatc tctccggtcg attcgaaagg
     1261 cgtccaggcg gcgataatta aagccgataa ggccggtatt cccgttatca ccgtcgacgt
     1321 cgctgcggaa ggcgttccgg tcgtatcgca tgttgcgacc gataactatg cgggaggcgt
     1381 tgaagcaggc aagttaatgg gcaaactttt gaatggtaaa ggtactgtag ccattatttc
     1441 ttatccggca ctacaatcgg tggttgcgcg cgttgatggt tttaagaaag ggttaagcga
     1501 tacaccggat attaaaattg ttgctgaaca gcctggtatt acgcgggctg aagcgttaac
     1561 gactgcgcaa aacattatgc aggcaaatgc caacctgaat ggtctgtttg gctttggtga
     1621 tgatgcggcg ctggcagcgg ttatcgcagc aaaatctgcg cataacgaca acatcaaaat
     1681 cattggtttt gacggcatga aagaagcgcg tgatgccgtt gacagtgaaa aaacgtttgc
     1741 ggccgtgatt cggcaatatc ccgatcaaat gggtgctaaa gccattgatg ccgctgttga
     1801 tcacctcaat ggtaaaccgg tagaaaaaat gatcccggta caacccggcg tttatacagg
     1861 taaatagtaa acatcttcac ctcacaacaa aacgttattg gcgttttgtt gtgacaccga
     1921 ggtcattatg acggatgtta ttcttgatgt ttcgcatatt gcgaagacat ttggtcatgt
     1981 tcaagcatta aaagatatta ccctctcttt acgtaaaggg cgggtgcata ctttattagg
     2041 cgagaatggt gccggtaaat cgacattaat gaaaatactg gctggtgttt atccgccaac
     2101 ccagggaacg ataacactac gtggtgaaac tatcaccatt aataatccgc agcattcacg
     2161 ccagttagga attgcgatta tttttcagga attgagcctg agtaataata tgaccgtggc
     2221 ggaaaatatt tatgccaata atgaaccgcg acgttttggc attattaacg ataaaaaaat
     2281 gctcgcagat tgccagaatc tcctcgcgga actgggcata ccactagacc cactagaaat
     2341 ggtcggcaat atgtcgatgg cacataggca actggtagaa attgctaaag ccttaagcta
     2401 tgcagcggat gtcgtgatta tggatgaacc gacatcttcg ctaagtgata atgaagcgga
     2461 gattctgttt aacattattg aaaaattaaa gcagcgaggc tgtgcggtta tttatatctc
     2521 tcatcgcatg gatgaaatta tgcggatttc agatgatatt tcggtgatcc gcgacggtga
     2581 gtatatcgcc acgcatgaaa agaaaaacag tgatatccag catttgattg cccaaatggt
     2641 tggtcgggaa atgaaaaata tttggccagc acgtcttggg gaaataccgg atgaaaatgt
     2701 tcctgcaaaa ctggaagtaa aaaatctttc ccatccttcg ctttttaaag agatcagttt
     2761 cgctgtgcgt ccgggggagg tcctcggatt ttttggcctg gtgggggccg ggcgttccga
     2821 tgtgatgaag gcgctctttg gcctcgtttc gtatcagggg actgtgttga ttgatggtaa
     2881 ggaagtcagg atcgctaatc ctaaacaggc tattgatcat ggcattgctt ttgtgaccga
     2941 aaaccgcaag gaagaagggt tagtgctgat gcacgacgtc aatatgaaca cgcaccatgt
     3001 ggcgtttcaa tataacgctt cgcggatggg actcatcaat caccgacaag aagaggcgaa
     3061 gacgctgcaa tcaattgcca gaatgaacac taaagtgagt tctgtacatc aggcagtggg
     3121 agcgctgagc ggcggtaatc aacagaaaat cgtgttatcg aagtggctgg agaaaactcc
     3181 gcgtatttta ttgctcgatg agccaacgcg cggagtggat gtcggcgcaa aatttgaaat
     3241 ctataacgtg atacgtcaac tggctgcggc agggacagca attattctgg tgtcctctga
     3301 actgccagaa gtcatggcac ttagcgatcg tctggtggta atgcgtaata aaacgattgc
     3361 cgatatttat tcatgtgaaa atttaacgca gacacaggta atgaccgctg caacaggagt
     3421 acgttaaatg acagagaaaa ctcttgccag tccgaaacct tcggaaattc ccattgcaaa
     3481 taattttaaa aaagtcttca ggcaatatgg cggtatcctc agtggaatgg tggtgttaat
     3541 cattctgttc agttttatta atgacagttt ttttaccgcc aacaacatta ctaatattat
     3601 tcttcaggtt tcgataattg caattaccgc ctatggaatg acttatgtac ttttgcttgg
     3661 cgatattgat ttatcggtcg gttcaaccat tgcattgatt ggcacatttg ctgcgcttgg
     3721 ggcttcatgg ggaattccat ttattttatt ggtcccatta tccattattg cagcactggc
     3781 tctggggatg ataaatggtg gcttgacggc tatagcgggg atcccgtcgt ttattgtcac
     3841 tgttgctact atggggatct tccgtggtat cgcttatata gtgaccgatg gtatgcccat
     3901 catgattaaa gatgatgcct ttctggcact ggggaatgga gaatttctct atatacctat
     3961 tccaatctgg atattaatca tcttattgct gattaaccac ttcattctta ctaaaacaac
     4021 ctttggacga aaaatttata tcactggtgg taataaagaa gcggcaattt attcagggat
     4081 aaatgtcacc cgcttgaaaa ttaaagtctt tatgattaca gccgtgctgg caggtatcag
     4141 cgggatgatc ctcgcctcac gccgctattc cgggcaaccg aattccgcgt tgagctacga
     4201 actggatgct attgctgcgg ctgtactggg aggcaccagc ctgaacggcg gctatggaac
     4261 cgtggtgggg acggtgatcg gcgcattaac tatcggcgtt attaataatg gcatgaactt
     4321 aatgaatgtt ccctattttt accagatggt ggttaaagga ttagtcattc ttgtagccgt
     4381 ttattttgac gtaagaaata aaagaaaacg cagctaatca tttcaaggaa ttaatcatat
     4441 gagtaaggtc ttcacaattg gagaaattct ggttgaaatt atggcgagta aaatcggcca
     4501 gccatttgat caaccaggta tctggaacgg cccttatccc agtggtgcgc ccgctatttt
     4561 tatcgaccag gtgacacgtc ttggcgttcc gtgcggcatc attagctgtg tggggaatga
     4621 tggctttggt gatattaata tacatcgtct ggctgctgac ggtgtggata tcagggggat
     4681 ttcagtctta cctctggaag ctaccgggag tgcctttgtt acttatcata actctggcga
     4741 tcgcgatttt atttttaata ttaagaatgc cgcttgcggt aaattatcgg cgcagcatgt
     4801 tgatgaaaat attctgaagg actgtaccca ttttcatatt atgggctctt ccctgttttc
     4861 gtttcatatg gtagacgcag taaaaaaagc ggtaactatc gttaaagcaa atggtggtgt
     4921 tatttctttc gaccctaata ttcgtaaaga gatgctcgat attccagaaa tgcgtgacgc
     4981 cctccacttt gttcttgagt taacggatat ctatatgccc agcgagggcg aagtgttgct
     5041 cttgtcgcca cattcgacgc cggagcgtgc aatcgcaggt tttcttgaag aaggtgttaa
     5101 agaggtgatt gtgaagcgcg gcaatcaagg tgccagttac tactccgcga atgagcagtt
     5161 tcatgttgag agctatccgg tggaagaagt tgacccgacc ggtgcgggag actgctttgg
     5221 tggcgcgtgg attgcctgcc gccagctagg gtttgacgcc catcgagcgc tgcaatatgc
     5281 caatgcctgc ggtgcgctgg cggtaaccag acgtggccca atggagggaa cgtctcgcct
     5341 tgcggaaatc gaagcgttca tccagcgcca tgacatgtcc attcgggaag cagcacaatg
     5401 aaaaacgaaa tttggtcgtg gattggcgat ctctcgcagg ttgccggaat taaacactat
     5461 gagttacgta gtggcagagc aaagggtaca gaagcgtttg atgtccgtac tggtgcggga
     5521 ctggcattta cggtggtaaa agatcgcgcg ctggatattg catgggccag ctacaaggac
     5581 acggcgcttt cttttatcac ccccaacggc attgtcgccc ccgctttttt tgagtctcag
     5641 ggcaacggat ttttacgtag cttttacgcg gggttactga ctacctgcgg acttagctac
     5701 attggcacgc cttgtgagga tgaaggagaa acgctggggt tacacgggcg tttggctgcg
     5761 actccagcag aagaggtggg gtatcgcaca gaacggaccg acgacggcat tgaatttgtc
     5821 atcaatggca aagtacggga aacgcgactg ttcggcgaaa atctgacgct ggaaaggacc
     5881 attcgctgcc gttatggcga aaatgtgctg cgcattgaag acaaggtgac taatcatggt
     5941 ttcacccgcc agccgctgca aattctttat cactttaatt acggctggcc tttattatcg
     6001 ccacaggcag aaatcttgct gtcggctaaa agcgtaacgc cacgaactcc gcatgcggcg
     6061 gaagggttaa ccagtcatct ggagatttgt accccacagc ccggttttga cgagcaggtt
     6121 tactatctca cgttgaatag cgacagtcag gggatgagta aagttgctct ggtgaacgcc
     6181 gaactggggt ggggaattta cgagaaattc gacaccatgc agctacccaa ctttatccag
     6241 tggaaaaatt taggcgcggg tgagtatgtt atgggactgg aggtcagtaa tagttttcct
     6301 gacggtcgtg ataaagaacg agcgcagggg cgtctgccct ttattgagcc aggggaaaca
     6361 aagaaatact gctttgagct gggaattgtt gatggcgatg ctgaaatgag cgcgttgaaa
     6421 gcggaaattg caggctaccg ttagctaacg gcaataaaaa cgccgggcag cgttaacagg
     6481 cccggcgttg attacgttct ttgctaagcg cgatgaaaac accgcgcgtc acaaattatt
     6541 tttcctgaag accgagtttt ttctccagat agtggatgtt agtgccacca tgctggaagt
     6601 tctcgtcatt catgatgcgg atctgcagat caacgttggt tttgataccg tcgatgatca
     6661 gctcctgcag cgcattcttc atgcgggcaa tcgccacgtc acggttttca ccgtagcaaa
     6721 tcagcttacc gatcattgag tcatagtacg gcggtacggt gtagcccgcg tagatatgag
     6781 actcccaacg tacgccaaaa ccgccaggtg cgtggaaacg ggtgattttg cctgggcttg
     6841 gcaggaaggt gttcggatct tcggcgttga tacgacattc caccgcatgg ccgcgaacgt
     6901 gaacttcttc ttgcttgatc gacagcggtt gaccggcagc gatacgcagc tgttctttga
     6961 tcaggtcaac gccggtgatc atttctgtaa ccgggtgttc tacctgaata cgggtgttca
     7021 tttcgatgaa atagaactcg ccgttttcga acaggaactc gaaagtacct gcaccgcgat
     7081 agccgatatc aacacacgct ttagcgcaac gttcgccgat gtagcgacgc agttccgggg
     7141 taatgcccgg tgctggtgct tcttcgacca ctttctggtg gcggcgctgc atggagcagt
     7201 cacgttccgc cagatagata gcgttgccct gaccgtcagc cagtacctga atctcgacgt
     7261 ggcgaggatt ttccaggtat ttctccatgt aaaccatatc gttgctgaaa gcagctttcg
     7321 cttccgcacg ggtcatggag atggattgtg ccagttcagc gtcgccgcgc actacgcgca
     7381 taccgcgacc accgccgccg ccggaggctt tgataatcac cggataacca atgcgtttag
     7441 caatggcacg gtttttatcc atatcgtcgc ccaacgggcc gtcagaaccc ggtacgcaag
     7501 ggacgcccgc ttttttcatc gccgcgattg cggatacttt gtcgcccatc aggcgaatgg
     7561 tttctgcttt cgggccgatg aagataaagc cggagcgttc aacctgctcg gcaaagttgg
     7621 cgttctcgga gaggaagccg taacccggat ggattgctac tgcgccggtg atttcagcgg
     7681 cgctgatgat tgccgggatg ttcagataac tttttactga cggagcaggg ccaatacaga
     7741 ccgtttcatc tgccagtaat acgtgtttta gatcgcgatc cgcgctggag tgcacagcga
     7801 cagtcttgat gcccagttct ttacaggcac gaagaatacg caatgcaatc tcgccgcggt
     7861 tggcaataac aattttatcc agcatgttcg cctcgttact cgatgacgac cagcggctcg
     7921 tcaaattcta ccggttgtcc actttcgacc agaattgctt tcacggtacc ggatttgtcc
     7981 gcttcgatct ggttcatcat tttcatggct tcaacgatgc acagggtatc gcccacgttg
     8041 actttctgac ccacttcgat gaacgctttt gcgtccgggc ttggggtgcg gtagaaagta
     8101 ccaaccatcg gggaacgtac gatgtgacca ctgatttccg ctgctgctgg cgcttccatg
     8161 gaaggaacgg tcgccggagc ggctgcgtta gattgagctg gctgctgcat tattggtgca
     8221 gcgtaagctt gttgcatcac agggaaactt gcggcaggag ctgcacggct aatgcgtact
     8281 gactcttcgc cttcagaaat ttccagttcg gagatgcctg attcttcaac cagctcgatc
     8341 agttttttaa tcttacgaat atccatgagt gggttccgta ctctttgttt agtgtgattg
     8401 tgacaggcgt ttcaccgccg tctgtaaagc gtatgccccg acagctgtat gcatagcgat
     8461 aaattccagc aggccgggac gccggtctat tttgccccag agtgtcaatg ttagacttga
     8521 cggacattgt gcagcgatct gctgctatcc tccggcaaaa aacaaaatat accttcgttg
     8581 cgccaatgtc accttttccg cagcgcaaaa actgcgtcag ggaggacgag gtcgcacatt
     8641 ataacgattt cgtagcaatt ggcagctaaa tactggtctt atcagggaag ataatcaaca
     8701 gctaacatgt aaataacctt caacaccgtg taatttgcaa caagccgcac aattcacgaa
     8761 attagcgcca tcatcgacgg aacttcttat aacgtaaggt gaaaagctgt aaagccagcc
     8821 ctgcgaagat gaggggctgc aatgagataa tcttcacaga ccacaaataa tgtagggatg
     8881 ccaggatcgc gacaagatag acgacattac gcagcggttg ctggggatta cccaattttc
     8941 actgcattgt ctggatagat gttaatgcta aagcacgcaa aattaaccga ttgacaatgc
     9001 tcagcattaa ataggttcga gtgattaatt tgtagtcaaa caaaatcaga ttattccccc
     9061 cattttgcag aaggtaaact gttttgtcag gcggcagtgc ggctatctga tgctttcaga
     9121 atttgccacc tgacaataaa aaagccgatt tggtagcaaa atcggcttcg gtgagtttgc
     9181 agttttcatc acatactccg acaagctttt tcaagcatga cggaggttgc gttaatgcaa
     9241 ggtttctacc cagggataaa agtgaccgtt acggcggatg aatatctgtg ttgtcagagg
     9301 atggtcatcc tcacggaagc attgttttgt tagctgtcgt aatgcactga gatttgctgg
     9361 ctgatcattg tgggtgaaaa acagttttcc tctggaggga acggtcacta acaggtcgcc
     9421 gcattcagga tcgggtgatt gcacccaaag atcgtcaatg agcatcagac tgggctcata
     9481 agtaccccct gcggagataa cggcaacttt tccatcgttt gcgcctgtat caacgatttc
     9541 agccgtaact tccggcaata tttgcttcaa attactgatg gcttgttcgc ggtcaatggt
     9601 cacttcgtag cgcatcagat agcgcatggt ttcgctacca tcttcagcaa acaagataat
     9661 taactcatcg ttaaagtctt cccaggcaaa atccagcgag gcgttgagga cctctggatc
     9721 agactcatac tggctaagct gttggcgaac ggtatccagc caggcttttt ctttaatcac
     9781 cggaacaata cagcgcccgg tcggcagcat ttctgcaagc tgtttcagca tatctgcagc
     9841 cacttcttgc agcgggtctg gtgcggcttg ataacgtttg taggcatcat caagggtgat
     9901 ttccaccgtt tggtgacccg tatcaatgcg catcaatttg tggtcagtcg aaatcatgac
     9961 tttccagttt ggatgatgtt gtacaacttc ctgaccaagg tgttcgacga aagctgtccg
    10021 gctaaccgct tttcccgtta accgattgaa taatgatgtc aaaatgagtt ccctctctta
    10081 ttattccttg ctaaatggtt agttaacctt caccagcgtg cgaccctgga tctggttatt
    10141 aatgatggcc tcggcaaagt tcggtgcctc tgacagagat atctcttttg ccgcctgggt
    10201 atagaatgat tccggtaaat cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg
    10261 tggcgtcatt actgaatcca ccccttgcaa acggacatta cgcagaataa atggcatgac
    10321 cgtggttggc agagtaaaac cacccgccag accacaggca gccacgcagc cgccgtaatt
    10381 catttgcgcc agcacttttg ccagcacttt gtcgccaaca gtgtcaattg ccccagccca
    10441 gacttgtttt tccagaggac gggattcggc aaactcatca cgagggagaa tacgactggc
    10501 acctaaactt ttcagatatt catgggtact ttcgcgaccg gaaacggcaa cgacctgata
    10561 acccaactta tgcagcagcg ccacggcggt actgccgacg ccaccactgg cacccgtcac
    10621 gacaatctcc ccgtcctgcg ggcgaacacc ggcatcttcc agcgccatca cacacagcat
    10681 ggcggtaaaa ccggcagtac cgataatcat tgctttacgc gcgtccagcc cttgcggcat
    10741 ggcaaccagc cagtcacctt tcactcgcgc ctgctccgcc agcccacccc agtggttttc
    10801 accaacgccc cagccagtga gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct
    10861 ggtgcgtaca gttccggcaa aatcgatccc aggaatcatc ggaaaattac ggatgatttt
    10921 tcccttaccg gtaatcgcca gcgcatcttt atagttcagg ctcgaccagt gaacatcaac
    10981 cgtgacatcg ccctccggca ggcgactttc gtcaagtgtc tgtaccgatg cgagagtttt
    11041 gccgtcctgc tgttctaaaa gtaacgcctg cataagtggt cctcatgtgc atgatggatt
    11101 ggaaaataat ttctgaagac tatactcgct aatggaaaag caaagccgat gaagcgcaat
    11161 aaattgcgag ataaatctga tttgctagta tgcccgcttc ctcactatcg gagttaacac
    11221 aaggatgaga ttaacgacga aattttcggc ctttgttacg ctgctcaccg ggttaacaat
    11281 ttttgtgact ttgctgggct gttcgctaag tttctacaac gcaattcagt ataagtttag
    11341 tcatcgcgtt caggcggtgg cgacggcgat tgatacccac cttgtgtcga atgacttcag
    11401 cacattaagg ccacaaatta ccgaattaat gatgtcggca gatatcgttc gtgtagacct
    11461 gctccatggt gataaacagg tttataccct ggccagaaat ggtagttatc gtccggttgg
    11521 ctccagcgat ctgtttcgcg aactgagtgt tccgttgata aagcatccgg ggatgtcgct
    11581 gcgtctggtt tatcaggatc cgatgggcaa ctatttccat tctttaatga ctaccgcgcc
    11641 gctcacgggg gcgattggct ttatcattgt tatgctcttc ctggcggtac gctggttaca
    11701 acggcaactt gccgggcaag aattgctgga aacccgggct actcgtatct taaacggtga
    11761 gcgtggctct aatgtgttgg gaaccatcta tgaatggccg cccagaacca gcagtgcgct
    11821 agatacgctg cttcgtgaaa ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct
    11881 gatccgctct tatgccgccc aggacgtgaa aaccggcctc aataaccgac tctttttcga
    11941 taatcagtta gcaacgttac tggaagatca ggagaaagta ggtacccacg ggatcgtgat
    12001 gatgattcgt ctgccggatt tcaatatgtt gagcgatact tgggggcaca gccaggttga
    12061 agaacagttc ttcactctga cgaatctgct gtcgacattt atgatgcgct accctggcgc
    12121 actgctggcg cgttaccacc gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa
    12181 agaggcagag agcatcgccg gtcagttaat caaagccgtc gataccttgc cgaacaataa
    12241 aatgctcgat cgcgacgata tgatccacat tggtatctgc gcctggcgta gtggtcagga
    12301 taccgagcag gtaatggaac atgcagagtc tgccacgcgt aatgcgggat tgcagggcgg
    12361 caatagctgg gctatttacg atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg
    12421 gcgtacgctt atcgagcaaa tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc
    12481 ggttactcgc gaaggtcagg ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa
    12541 tgaagaggtt agctcggcgg agtatatgcc gatggtcttg cagtttggct tatcggaaga
    12601 gtatgaccgt ctgcaaatca gccgtcttat tccactattg cgttactggc cagaggaaaa
    12661 tctggcgatt caggttaccg ttgagtcgct gattcgcccg cgttttcagc gttggctgcg
    12721 cgatacgtta atgcaatgtg aaaaatcaca acgaaaacgc ataattattg aacttgcaga
    12781 ggccgatgta ggtcaacata tcagtcgttt acaacctgtt attcgtttag tgaatgcttt
    12841 aggggtacgg gtagccgtca accaggctgg tttgacgctg gtaagcacca gttggatcaa
    12901 agaacttaat gttgagttac tcaagctcca tccggggctg gtcagaaaca ttgagaagcg
    12961 aacggagaac cagctgctgg ttcaaagcct ggtggaagcc tgctccggga ccagcaccca
    13021 agtttacgcc accggtgtgc gttcgcgaag cgagtggcag accctgattc agcgcggtgt
    13081 tacaggcggg caaggggatt ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa
    13141 atattcacaa agatactcgg tttaacctgc cgtttaatct gttttcacgt agaataacgc
    13201 gcgctgcgtc tcatgggagt gtgcttgtct gctcgccaga ttgttgcagc acatatgcag
    13261 atgaatgacc ttacgcggtt gcaaacaggc gaggaatgct gctgatgcat taagcctttc
    13321 tggactcagg cagagatttg taacaaagga aacgaactgc actaattttc accgtagcag
    13381 atgatttttg cgccttgtcg ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc
    13441 cgccccagct ttcaggatta tcccttagta tgttgaaaaa atttcgtggc atgttttcca
    13501 atgacttgtc cattgacctg ggtactgcga ataccctcat ttatgtaaaa ggacaaggca
    13561 tcgtattgaa tgagccttcc gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa
    13621 gcgtagctgc agtaggtcat gacgcgaagc agatgctggg ccgtacgccg ggcaatattg
    13681 ctgccattcg cccaatgaaa gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc
    13741 tccagcactt catcaaacaa gtgcacagca acagctttat gcgtccaagc ccgcgcgttc
    13801 tggtttgtgt accggttggc gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc
    13861 agggcgctgg tgcccgtgaa gtcttcctga ttgaagaacc gatggctgcc gcaattggtg
    13921 ctggcctgcc ggtttctgaa gcgaccggtt ctatggtggt tgatatcggt ggtggtacca
    13981 ctgaagttgc tgttatctcc ttgaacggcg tggtttactc ctcttccgtg cgcattggtg
    14041 gtgatcgttt cgacgaagct atcatcaact atgtgcgtcg taattacggt tctctgatcg
    14101 gtgaagccac cgcagaacgt atcaagcacg aaatcggttc ggcttacccg ggcgatgaag
    14161 tccgtgaaat cgaagttcgt ggccgtaacc tggcagaagg tgttccacgc ggttttaccc
    14221 tgaactccaa cgaaatcctc gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg
    14281 taatggttgc actggaacag tgcccgccgg aactggcttc cgacatctcc gagcgcggca
    14341 tggtgctcac cggtggtggc gcactgctgc gtaaccttga ccgtttgtta atggaagaaa
    14401 ccggcattcc agtcgttgtt gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca
    14461 aagcgctgga aatgatcgac atgcacggcg gcgacctgtt cagcgaagag taatcggatg
    14521 caggcagggg aaatgtctgt ttaccctgcc tggtctgata cgagaatacg cataacttat
    14581 gaagccaatt tttagccgtg gcccgtcgct acagattcgc cttattctgg cggtgctggt
    14641 ggcgctcggc attattattg ccgacagccg cctggggacg ttcagtcaaa tccgtactta
    14701 tatggatacc gccgtcagtc ctttctactt tgtttccaat gctcctcgtg aattgctgga
    14761 tggcgtatcg cagacgctgg cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg
    14821 tcaggaactg ttgctgaaaa acagtgaact gctgatgctt ggacaataca aacaggagaa
    14881 cgcgcgtctg cgcgagctgc tgggttcccc gctgcgtcag gatgagcaga aaatggtgac
    14941 tcaggttatc tccacggtta acgatcctta tagcgatcaa gttgttatcg ataaaggtag
    15001 cgttaatggc gtttatgaag gccagccggt catcagcgac aaaggtgttg ttggtcaggt
    15061 ggtggccgtc gctaaactga ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct
    15121 gccaatccag gtgctgcgca acgatatccg cgtaattgca gccggtaacg gttgtacgga
    15181 tgatttgcag cttgagcatc tgccagcgaa tacggatatt cgtgttggtg atgtgctggt
    15241 gacctccggt ctgggcggtc gtttcccgga aggctatccg gtcgcggttg tctcttccgt
    15301 aaaactcgat acccagcgcg cttatactgt gattcaggcg cgtccgactg caggactgca
    15361 acgtttgcgt tatctgctgc tgctgtgggg cgcggatcgt aacggcgcta acccgatgac
    15421 gccggaagag gtgcatcgtg ttgctaatga acgtctgatg cagatgatgc cgcaggtatt
    15481 gccttcgcca gacgcgatgg ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc
    15541 gactccgcag caaccgacga caggaaatgc agctactgcg cctgctgcgc ccacacagcc
    15601 tgctgctaat cgctctccac aaagggctac gccgccgcaa agtggtgctc aaccgcctgc
    15661 gcgtgcgccg ggagggcaat agtggcgagc tatcgtagcc agggacgttg ggtaatctgg
    15721 ctctctttcc tcattgcgct gttgctgcaa atcatgccct ggccggataa cctgattgtt
    15781 ttccggccaa actgggtgtt actcatcttg ttgtattgga tcctggcctt gcctcatcgc
    15841 gtaaatgtgg gcacaggttt tgtgatgggt gccatactgg atctgatcag cggctcgacg
    15901 cttggcgtac gcgtattggc gatgagcatc attgcttacc tggtggcgct gaaataccag
    15961 cttttccgca acctcgcatt atggcagcag gcgctggtcg tcatgttgct ttcgctggtg
    16021 gtggatatta ttgttttctg ggcagagttt ttagtgatta acgtctcttt cagaccggaa
    16081 gtgttctgga gtagtgtagt caatggggtg ctctggccgt ggattttctt gctgatgcgt
    16141 aaagtccgtc agcagtttgc agtgcaataa aggtttctat gacttctctg tatttagctt
    16201 ccggttctcc gcgtcgtcag gagttacttg cgcaacttgg cgtgaccttt gaacgtattg
    16261 ttacgggcat tgaggagcag cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc
    16321 tggcgcgcga gaaagcacag gcgggtgtcg cgcaaacggc gcaggatctc ccggtgctgg
    16381 gtgcggatac tatcgttatc ctgaatggag aagtgctgga gaaaccgcgc gacgcagagc
    16441 atgcggcgca gatgttgcgc aaattatcgg gtcagaccca tcaggtgatg acggcagtgg
    16501 cgttggccga cagccagcac attctcgatt gcctggtggt caccgatgtg actttcagaa
    16561 cgttaacaga cgaagacatc gcgggctatg tcgccagcgg tgaaccgtta gataaagcag
    16621 gtgcatacgg tattcagggg ctgggtggct gttttgtcag gaagataaat ggcagctatc
    16681 acgccgtagt cggcttaccg ctggttgaaa cgtatgaatt attaagtaat tttaacgcac
    16741 tgcgtgagaa aagggataaa catgacggct gaattgttag taaacgtaac gccttcggaa
    16801 acgcgagtgg cgtatattga tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg
    16861 cgacgcggaa tagtaggcaa tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg
    16921 caggcggctt ttgtagatat tgggctggat aaagccgcgt ttcttcatgc atccgatatc
    16981 atgccgcaca ccgaatgtgt ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc
    17041 tcggaactgg ttcgtcaggg gcaagatctg atggtgcagg tggtgaaaga tccgcttggc
    17101 actaaaggtg cgcgcctgac caccgatatc acgctgcctt ctcgctatct ggtgtttatg
    17161 ccaggggctt ctcacgttgg ggtttcccaa cgtattgaaa gcgaatcaga acgtgaacgc
    17221 ctgaaaaaag tggtcgcaga gtattgcgac gagcagggcg ggtttatcat ccgtaccgca
    17281 gcggaagggg ttggcgaggc tgaactggcc tccgatgccg cttatctgaa acgcgtctgg
    17341 accaaagtta tggagcgtaa aaaacgcccg cagacccgtt atcagctgta cggcgaactg
    17401 gcgctggcgc agcgtgttct gcgtgatttc gccgacgccg aactggaccg cattcgcgtt
    17461 gactcacgcc tgacttacga agcattgctg gagtttacct cggagtacat tcccgagatg
    17521 accagcaagc tggagcatta caccggacgc cagccgattt tcgatctctt tgatgtcgaa
    17581 aacgaaatcc agcgagcgct ggaacgcaaa gtagaactga aatccggtgg ttatctgatt
    17641 atcgaccaga ccgaagcgat gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat
    17701 cgcaatctgg acgacaccat tttcaatacc aatattgaag cgacgcaggc tatcgctcgc
    17761 cagttacggt tgcgtaatct gggtgggatt atcattattg atttcatcga tatgaataat
    17821 gaagatcacc gccgccgcgt gctgcactcg ctggagcagg cgttgagcaa agaccgggtg
    17881 aaaaccagcg ttaatggttt ttcggcgctg gggctggtgg agatgacgcg taaacgcacc
    17941 cgcgaaagca ttgagcacgt actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg
    18001 gtgaaaaccg tggaaacggt atgctatgaa atcatgcgcg agattgttcg tgtccaccat
    18061 gcttacgact ccgaccgttt cctggtctat gcatctccgg cagtagctga agccttgaaa
    18121 ggcgaagagt cacactcgct ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta
    18181 caaattgaac cgctctataa ccaggagcag tttgacgtcg taatgatgta aacagatgct
    18241 gggccgccat ccggcaaagg gtttttgagt cacattttta gcagacaagg agtgacgggt
    18301 gaggcgattg ccggggattt tactgcttac tggagccgcg ctcgttgtga tcgctgctct
    18361 gctggttagc ggcctgcgta ttgctttacc gcatcttgac gcctggcgtc cggaaatcct
    18421 caacaaaata gaatccgcga ctggcatgcc ggtagaagcc agtcagctct cagccagctg
    18481 gcagaatttt ggcccgacgc ttgaagcaca cgacatccgt gcagaactaa aagatggcgg
    18541 cgaattttcg gttaaacgcg ttactctggc gctggatgtc tggcagagcc tgttacatat
    18601 gcgctggcag tttcgcgacc tcactttctg gcagctgcgc tttcgcacca acactcctat
    18661 caccagcggt ggtagcgatg acagtctgga agccagtcac atcagcgatc tgtttcttcg
    18721 tcaatttgac catttcgatc tccgcgacag tgaagtcagt tttctgacgc catccggtca
    18781 gcgcgccgag ctggcgatcc cacaactcac ctggctgaac gatccacgtc gacaccgtgc
    18841 ggaaggcctg gtaagcctct ccagccttac cggacagcac ggcgtgatgc aggtgcgcat
    18901 ggatttgcgc gatgatgagg ggttgttaag caatggtcgc gtctggctac aggcggatga
    18961 catcgacctg aagccgtggc tcggtaaatg gatgcaggac aatattgctc tggaaacggc
    19021 acagttctcc cttgaaggct ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt
    19081 ctggctgaaa cagggcggtg ccagctggtt gggcgagaag caaacgcata cgctgtcggt
    19141 ggataatctg accgcgcata ttacgcgtga aaatccgggc tggcagttct ctattcccga
    19201 tacacggatc acgatggacg gcaaaccctg gccgagcgga gcattgacgc tggcctggat
    19261 accggaacag gacgttggcg gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc
    19321 cagtaatctg gagctggcag gcctggaggg catacgcccg ctggccgcga aactttcacc
    19381 tgcactgggt gatgtttggc gctccacaca accgagcggc aagattaaca ctctggcgct
    19441 ggatatcccg cttcaggcgg cagacaagac ccgttttcag gcatcgtgga gcgatctggc
    19501 ctggaagcaa tggaaattat taccgggtgc ggaacacttc tccgggacgc tttccggcag
    19561 cgttgaaaat ggtttgctta ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt
    19621 attccgtgcg ccactggaaa tcgccgacgg ccaggcaact ataagctggc tgaacaatga
    19681 caaaggtttc cagctggatg ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg
    19741 cggcggtttt cgttacctgc aacctgctaa cgatgaaccc tggctgggta ttctggctgg
    19801 catcagtacc gatgatggtt cacaagcctg gcgctatttc ccggaaaact tgatgggtaa
    19861 agacctggtt gattacttaa gtggcgcgat tcagggcggt gaagcggata acgcgacgct
    19921 ggtttatggt ggcaatccgc aactcttccc ctataaacac aacgaaggtc agtttgaagt
    19981 gctggtgccg ctgcgcaacg cgaagtttgc cttccagccg gactggcctg cattaactaa
    20041 ccttgatatt gaactggact ttattaacga cggtttatgg atgaaaaccg atggcgttaa
    20101 tctgggcggc gtgcgcgcga gtaatctcac cgcagtgatc cctgactact cgaaagaaaa
    20161 actgctgatt gacgctgaca ttaaaggtcc gggtaaagcc gttggccctt actttgatga
    20221 gacaccgctg aaagattctc tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt
    20281 gaatgctcgc ttacatcttg atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga
    20341 agtgacgctg cgtaataaca gtctgtttat caaaccactc gacagcaccc tgaaaaattt
    20401 gagcggtaaa ttcagcttta tcaatggcga tctgcaaagt gaaccactga cagcaagctg
    20461 gtttaatcag ccgttgaacg tggatttttc caccaaagaa ggggcaaaag cctaccaggt
    20521 tgcggtgaat ctcaacggta actggcaacc ggcgaaaacc agcgttctgc ctgaagcggt
    20581 gaacgaagca ttgagtggca gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta
    20641 tcatgctggc gcgacctata acgtagagct gaacggcgat ctgaataatg tgagtagtca
    20701 cttaccttca ccgttagcca aacctgcggg tgaaccacta gcggtaaacg ttaaggttga
    20761 tggcaatctc aacagctttg aattaaccgg acaggctggt gcggataacc atttcaatag
    20821 ccgctggttg cttggtcaaa agctgacgct cgatcgtgct atttgggcgg cagacagtaa
    20881 aacgctcccg ccgttgccgg aacaaagtgg cgttgaactc aatatgccgc cgatgaatgg
    20941 tgccgagtgg ctggccctgt tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag
    21001 tttcccacaa cacataacgt tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa
    21061 taacctgagt attgtttcgc aaccgacggc aaatggcacc ctggttgagg cgcaagggcg
    21121 tgaaatcaac gccacgctag cgatgcgtaa taacgcgccg tggctggcga atatcaaata
    21181 tctttattac aacccgagcg tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt
    21241 cccgacaacg gagcgcatta acttccgtgg ctggccggac gcacaaatac gatgcacaga
    21301 gtgctggttc tgggggcaaa aattcggtcg cattgacagt gatatcacca tttctggcga
    21361 tacgttaacg ctgaccaatg gactgattga tactggtttc tcgcggctta ctgccgatgg
    21421 tgaatgggtt aataatccgg agaatgaacg tacctcgctg aaaggaaaac tgcgcgggca
    21481 gaaaattgat gccgccgcag aattttttgg tgtcacgacg cccatacgcc agtcgtcatt
    21541 taatgtggat tacgatttac actggcgcaa agcaccctgg caaccagatg aggcgacgtt
    21601 gaatggcatc attcatactc aactgggtaa aggcgaaatt accgaaatca ataccggaca
    21661 tgccgggcaa ttgctgcgct tattgagcgt tgatgccctg atgcgtaagc tgcgttttga
    21721 tttcagagac acttttggcg aagggttcta ttttgactcc attcgcagca ccgcgtggat
    21781 taaagacggg gttatgcaca ccgacgacac gctggtggat ggcctggagg cggatatcgc
    21841 catgaaaggg tcggtaaatc tggtacgtcg cgacctgaat atggaagcgg ttgtcgcacc
    21901 agagatttct gcgacggtgg gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc
    21961 ggcagtattt gcagccagta aagtgctggg gccgctgtgg agcaaagtct ccattttgcg
    22021 ctatcacatt tcgggtccgc tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg
    22081 taaagaaaaa gcgcaatgat ttgacgaggg cgcgtaattg ccccaatctc atatgataat
    22141 cgttgccaaa ggccaacgag ccggaacata actgtaggcc ggatagggcg ttcacgccgc
    22201 atccggcagc cgtaaaaaat cctctactgc agtaactaac gagtagcaaa aacgatgagt
    22261 ctgaacctgg taagtgaaca attgctagcg gcgaacggcc tgaaacatca ggacttgttc
    22321 gcgatcctcg gtcaactggc cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg
    22381 agctatcacg aatcctgggt tttagaagac cgcattatta aagatggttc ttacaacatc
    22441 gatcagggcg ttggtgtgcg tgcaatcagt ggtgaaaaaa ccggatttgc ttacgctgac
    22501 caaatcagcc tgctggcgct ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat
    22561 agtggtgatg gcaaagtaca gacgctgggc gcggtagagc atagcccgtt gtatacctcg
    22621 gtagatccgc tgcaaagcat gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat
    22681 aaggttgccc gcgaagcgga caagcgcgta caagaagtga ctgccagcct cagcggcgtt
    22741 tatgaattaa ttctggttgc ggccaccgac ggcacgctgg cggcggatgt tcgtccgctg
    22801 gtgcgtcttt ccgtgagcgt tctggtcgaa gaagatggca aacgcgaacg cggtgccagt
    22861 ggcggcggcg gtcgttttgg ttatgagttc ttccttgccg atcttgacgg cgaagtccgt
    22921 gcggatgcat gggcaaaaga agcagtgcgt atggcgctgg tcaatctttc tgccgttgct
    22981 gcaccagcgg gcaccatgcc ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat
    23041 gaagcggtag gtcacggtct ggaaggcgac ttcaactgcc gcggcacttc agtatttagt
    23101 gggcaggtcg gggagctggt ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg
    23161 gtcgatcgcc gtggttcggt ggcgattgat gacgaaggta cgccaggcca gtacaacgtg
    23221 ctgattgaga acggcattct gaaaggctac atgcaggata aactcaacgc gcgtttgatg
    23281 gggatgacgc cgactggcaa cggtcgccgt gaatcctacg cccatctgcc catgccgcgt
    23341 atgaccaaca cctatatgct gccgggtaaa tcgaccccgc aggaaattat tgaatccgtt
    23401 gagtacggta tctatgcacc gaactttggt ggcggtcagg tggatattac atccggcaaa
    23461 ttcgttttct ccacttcaga agcgtatctg attgaaaacg gtaaagtaac gaagccggtg
    23521 aaaggcgcga cgttgattgg ttccggtatc gaaaccatgc agcagatttc gatggttggc
    23581 aacgacctga aactggataa cggcgtgggt gtctgcggta aagaagggca aagtttgccg
    23641 gttggcgtgg gccagccaac gctgaaagtt gataacctga ctgttggcgg tactgcgtaa
    23701 taatttcatt attttcagaa ggataattaa tctttctacg tgcacgaacg gtcccctcgc
    23761 tcctctgggg ttagggtgag gggaacccgt tggcacaggt ttgtagaacg taacagtaca
    23821 atatgaatta cttctctttc ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa
    23881 caaaataatc cgtcagcgag ttgatcacga cctgtacctt cagcggcagc ttatcttttt
    23941 cggtatataa cgcataaacc gggcgtggat ctgactgata acgcggcagc aggatctcca
    24001 gctcaccgcg attgatctcg ttgatcaccc acatcagcgg cacgtaggcg atcccggcac
    24061 ccgccgttag ccagcgcacc agcgtcatcg gatcattagt cacaaatctc ccttgcggga
    24121 tcaggcgagt cgagatccct tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc
    24181 tgtattcaag ccatgaatgg ctacttaaat cggcgggttt ttccggtatg ccgtattgtg
    24241 tgagatagct tttcgcggcg cacaccacca ttggcatcgc gccaagacgg cgggaaaaca
    24301 ggctggaatc ctgcaacgcg ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt
    24361 cgggggctgg aattccggta accagattga cgctcaaacc tgggtattct ttcagcattt
    24421 tggctgtcag cccggcgaga acattttgtg ccatagttga agaacagcca atgcgtagcg
    24481 tcccgatggg ggtgttattg aaggcataca gttgctcatg aacatcctgc acttcatgaa
    24541 gcatacgacg gcagccctgg tagtaaattc taccggcttc ggtcaggcca atgctgcgtg
    24601 tgctacggtt taacagcttt acctgcaact catcttccag ttttgacacc gtctgactga
    24661 tggacgaaac gctcatctgt agctgtctgg cggcggcggt aaaagagcca aattcaacta
    24721 ctttggcaaa caccgacatg cgttttagtc gttccattat tcactctgac ttaaaagtga
    24781 tttagatcac ataatataga taacagcata acagttacgc taatatatta aatatcaatc
    24841 tacagcaatg ttgctctcgc ccggctttcc atgcccttct ctgcggcgac agatgctgaa
    24901 aataataacg cctgctctct ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc
    24961 gtggtgtttg ggctgtcctt cccaccgata ttttttgaat tgcttttatc actggcgatt
    25021 ttctggctgg tgcgccgggt acttgtgcca acaggtatct acgactttgt ctggcatccg
    25081 gcgttgttca acaccgcgct ctattgctgc ttgttttatt tgatatcgcg actgttcgtt
    25141 tgaggttgaa gtgaaaacac taataagaaa attctcccgt acggccatca cggtcgtatt
    25201 agtcattctg gccttcatcg caatttttaa tgcctgggtc tattacaccg aatccccctg
    25261 gacgcgtgac gcgcgcttta gcgctgacgt cgttgcgatc gcgccggacg tttctggact
    25321 cattacccag gtgaatgttc atgataacca gctggtgaaa aaaggacaga tactgttcac
    25381 catcgaccag ccgcgctatc aaaaggcgct tgaggaagcg caagccgatg ttgcctatta
    25441 tcaggtactg gcacaggaga aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca
    25501 ggcgatgtct cgcgaagaga tcgaccaggc taacaacgta ctacaaacgg ttctgcatca
    25561 gttagcgaaa gcgcaggcga cccgcgatct ggcaaaactg gatcttgaac gcacggtgat
    25621 ccgcgcgcca gcagatggct gggtgaccaa cctcaacgtc tataccggtg agtttattac
    25681 tcgaggatca acggcggttg cgctggtgaa acagaactcc ttctatgtac tggcctatat
    25741 ggaagaaact aagctggaag gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg
    25801 cagtaacaaa gtgctgaaag ggactgttga tagtgttgcc gctggggtca ccaacgccag
    25861 cagcacgcgt gacgacaaag ggatggcgac catcgactct aaccttgaat gggtgcgttt
    25921 ggcgcaacgt gttccggttc gtattcgtct cgacaaccag caagagaaca tctggcctgc
    25981 gggcaccact gctacagtgg tggtcactgg caaacaagat cgcgacgaaa gccaggattc
    26041 gttcttccgt aaaatggccc atcgcctgcg tgagtttggt taatcacgat gggtattttc
    26101 tccattgcta accaacatat tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg
    26161 ctgtttgttg gctttcactt ccagctggaa acgccacgct gggcggtact gacagcggcg
    26221 attgttgccg ccggtccggc ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc
    26281 tatcgtggct ttttgcgcat catcggcaca tttattggct gtattgccgg actggtgatc
    26341 atcattgcga tgatccgcgc accattattg atgattctgg tgtgctgtat ctgggccggt
    26401 ttttgtacct ggatatcctc gctggtacga atagaaaact cgtacgcgtg ggggctggcc
    26461 ggttataccg cgctgatcat tgtgatcacc attcagccgg aaccattgct tacgccgcag
    26521 tttgccgtcg aacgttgtag cgagatcgtt atcggtattg tgtgtgcgat tatggcggat
    26581 ttgctctttt ctccgcgatc gatcaaacaa gaagtggatc gagagctgga aagtttgctg
    26641 gtcgcgcaat atcaattaat gcaactctgt atcaagcatg gcgatggtga agttgtcgat
    26701 aaagcctggg gcgacctggt gcgacgcacc acggcgctac aaagcatgcg cagcaacctg
    26761 aatatggaat cttcccgctg ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta
    26821 tcgctgacgc tgattaccca atcctgcgaa acttatctta ttcagaatac gcgcccggaa
    26881 ttgatcactg atactttccg cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc
    26941 cacaagcagc tcaaacgcct gcggagagtt atcgcctgga ccggggaacg ggaaacgcct
    27001 gtcaccattt atagctgggt cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt
    27061 atcagtaaca caaaaatcaa cgccaccgaa gaagagatcc tgcaaggcga accggaagtc
    27121 aaagtagagt cagccgaacg tcatcatgcg atggttaact tctggcgaac cacactttcc
    27181 tgcattctgg gcacgctttt ctggctgtgg acgggctgga cttccggcag tggtgcaatg
    27241 gtgatgattg cggtcgtgac gtcactggca atgcgtttgc cgaatccacg catggtggcg
    27301 atcgacttta tctacgggac gctggccgcg ctgccgttag ggctgctcta ctttttggtg
    27361 attatcccta atacccaaca gagcatgttg ctgctgtgta ttagcctggc agtgctggga
    27421 tttttcctcg gtatagaagt acagaaacgg cgactgggct cgatgggggc gctggccagc
    27481 accataaata ttatcgtgct ggataacccg atgactttcc atttcagtca gtttctcgac
    27541 agcgcattag ggcaaatcgt cggctgtgtg ctcgcgttca ccgttatttt gcttgtgcgg
    27601 gataaatcgc gcgacaggac cggacgtgta ctgcttaatc agtttgtttc tgccgctgtt
    27661 tccgcgatga ctaccaatgt ggcacgtcgt aaagagaacc acctcccggc actttatcag
    27721 cagctgtttt tgctgatgaa taagttccca ggggatttgc cgaaatttcg cctggcgctg
    27781 acgatgatta tcgcgcacca gcgcctgcgt gatgcgccga tcccggttaa cgaggattta
    27841 tcggcgtttc accgacaaat gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat
    27901 gataaacgtc gtcggtactt tggccagttg ctggaagaac tggaaatcta ccaggaaaag
    27961 ctacgcatct ggcaagcgcc accgcaggtg acggaaccgg ttcatcggct ggcggggatg
    28021 ctccataagt atcaacatgc gttgaccgat agttaagtca aaaccgacgc caaaagcgtc
    28081 ggttttttca tggctatact tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg
    28141 gcaacagatg aatatttata cctttgattt tgatgagatt gagagtcagg aggattttta
    28201 tcgtgacttt agccaaacct ttggtctggc taaagataag gtacgcgatc tcgactcact
    28261 atgggatgtg ttaatgaacg atgtcctgcc gctaccactt gagattgaat ttgttcatct
    28321 gggagagaaa acgcgtcgcc gttttggcgc gttaatattg ctgtttgatg aggcagagga
    28381 agagctggaa gggcatttgc gttttaatgt tcgtcattag cgcaaaaaaa agcccccgaa
    28441 ccgggggcaa tatcgtcgga caagacgatg agggtttatt tgtacagttc agccgtagcg
    28501 tgccaggtgt caccgctacg agcttcagta atctggtagg ccgttgcgcc tttctcttcc
    28561 gcttttttgt tcagcatttc acgcatatcc attggcgaag acgccacacc acttacggat
    28621 acggtcccga ttgcttcacg attttgtgct tgtgcagcat caatggagtc ggcagcgaat
    28681 gcaccgaaag agagaacaga aagtacgctt aatgcagcta cagtggtttt gattttcatg
    28741 atttttacct cgacataatc ttttagctgg gcctttgttt cgtgacccat atcacaaaat
    28801 caagtataca ctaattattg aactaattaa taccacgcta actgtttcag ttgatactaa
    28861 gtgttaaaaa tgtgaatttt aataacaaaa aagtataaaa aacacggtta tttattatta
    28921 attatagtat tttcaattag ataatgaatt ttgcataatg tgcttttttt accgtatgta
    28981 ttcattttgt gaatgatgtg tcgcggtaaa acgcactatt cgttaaatgt atcgcaggag
    29041 aaagaagggg aatgagaggg ataagcaaca ttttccccga cgccagaagc gacggggcag
    29101 agattaaagc tcctggtcga acagctctaa aattgcttcg tacaggtctt tgacggtgaa
    29161 accgttagca ggggtggtaa agatggtgtc atcgccagcg atggtgccca gaataccttc
    29221 tgctttgccc agtgagtcca gcaggcgagc aattaactgc gccgcgccag ggctggtatg
    29281 aatcacgaca actgcatcgt tgtagtcgat atccagcacc agattcttca atggactgga
    29341 ggtagttggt acacccagtt cagctggcag gcagtaaacc atttccattt tggcattgcg
    29401 tgtacgtaca gcaccaaact tggtcaacat ccgcgagact ttagactgat taatattgtc
    29461 aaagccttgc tcctgcaacg cggcgacgat ttcgccctgg gagctaaatt tctcttcttt
    29521 aagtaatgct ttaaatgctt taactagttc ttcttgctta gccgagcttc gcataagtca
    29581 cccgatatgg tggttgatac aacattattg tgcatacaga tgaattttta tgcaaacagt
    29641 cagccctgaa gaaggctgaa ataatgttat gaaagagcgg gattttatca aatttcgtta
    29701 ttgagaaaca tgcctgcgtc acggcatgca aattctgttt aaaagtaaat taattgttat
    29761 caaattgatg ttgttttggc tgaacggtag ggtatattgt caccacctgt tggaatgttg
    29821 cgctaatgca taagcgactg ttaattacgt aagttaggtt cctgattacg gcaattaaat
    29881 gcataaacgc taaacttgcg tgactacaca ttcttgagat gtggtcattg taaacggcaa
    29941 ttttgtggat taaggtcgcg gcagcggagc aacatatctt agtttatcaa tataataagg
    30001 agtttaggat gaaagtcgca gtcctcggcg ctgctggcgg tattggccag gcgcttgcac
    30061 tactgttaaa aacccaactg ccttcaggtt cagaactctc tctgtatgat atcgctccag
    30121 tgactcccgg tgtggctgtc gatctgagcc atatccctac tgctgtgaaa atcaaaggtt
    30181 tttctggtga agatgcgact ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag
    30241 gtgtagcgcg taaaccgggt atggatcgtt ccgacctgtt taacgttaac gccggcatcg
    30301 tgaaaaacct ggtacagcaa gttgcgaaaa cctgcccgaa agcgtgcatt ggtattatca
    30361 ctaacccggt taacaccaca gttgcgattg ctgctgaagt gctgaaaaaa gccggtgttt
    30421 atgacaaaaa caaactgttc ggcgttacca cgctggatat cattcgttcc aacacttttg
    30481 ttgcggaact gaaaggcaaa cagccaggcg aagttgaagt gccggttatt ggcggtcact
    30541 ctggtgttac cattctgccg ctgctgtcac aggttcctgg cgttagtttt accgagcagg
    30601 aagtggctga tctgaccaaa cgtatccaga acgcaggtac tgaagtggtt gaagcgaaag
    30661 ccggtggtgg gtctgcaacc ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc
    30721 tggttcgtgc actgcagggc gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg
    30781 gtcagtacgc ccgtttcttc tctcaaccgc tgctgctggg taaaaacggc gtggaagagc
    30841 gtaaatctat cggtaccctg agcgcatttg aacagagcgc actggaaggt atgctggata
    30901 cgctgaagaa agatatcgcc ctgggcgaag agttcgttaa taagtaatta attagcgaat
    30961 aataaaaaac cggagcacag actccggttt tttgttttga gcactcgact taattggttg
    31021 ccggatattc ctgaatggtg acctgcagcg ttaactgctt atcatcacgc atcactacaa
    31081 ccgggatcac cgaaccaggg cgaatttctg ccacctgatc catcgtctcc agagcagaga
    31141 tggccggttt gttatccacc gaaataatca gatcgttgac ctgaataccc gcattcgccg
    31201 ccgggccgtc aggtgacact tcattaacca cgatcccttg cagttgatct ataccaccgc
    31261 cctgcgcgtg cagtggtgcg atctcgcgtc cgccgatacc aatgtagccg cggatcacgc
    31321 gaccatcgcg gatcagctta tccataattt tggttgctaa ctggaaagga atcgcaaagc
    31381 cgataccttc cggcgtttcg ccatcgttac tcttatcaaa cgacagcgtg ttaatgccca
    31441 tcagttctcc cagcgagttc accagcgcgc cgccagagtt accgtggtta atggaagcat
    31501 cggtttgcag gaagttttgc cgcccggtcg ggttcagacc gattcgaccc gtagcactaa
    31561 taatcccctg ggtaatggtc tgcccgaggt tgtacgggtt accgatcgcc agtaccacgt
    31621 cgccaatgtg cggtacgcga cgtgcattga tcggaatggt aggtaaaccg ccagtggcat
    31681 taattttaag taccgccaga tcggttagag agtcagatcc caccagcaat gcttcaaata
    31741 cacgtccatc ctgtaaggcg acgatgatct gatcggcgtc gttgatgacg tgtttattgg
    31801 tgatgatata accgcgttga tccatgatta caccggatcc cagggtgcgg atctcaagct
    31861 ggttgtgaga gttggtgttc aaaccacggt tgtaaacgtt aaccaccgct ggcgcggcgc
    31921 ggcgaaccgc cagattataa ctggcaggcg tctcatcggt actgtcaaat tgcggagtgg
    31981 aaagcgggtt aaggctgcgc agcgaaggca tggcaaccag cagaatagcg ccgacaatta
    32041 atccaatcgc aacggaacgt aagagcttca caaacatgat ggaggcgtca ttaaaaaagg
    32101 gaacggcagc agcataccac gagttaaccg gacatcacac gtaagcctga tgcccggttt
    32161 acgacattaa cgcatcagca gatagatgct ttcattaccg cgtacaattt gcagggcgat
    32221 gatggccggt tttgccgcca gcactttacg catttcagca atcgagttca cccgatcgcg
    32281 gttgacgcca atgatcacat cgtctttttg caagccagcc tgagcagctg ggcttccttt
    32341 gacaacttca tcgatcttaa tacctttgcc gccatctttt agctgaccat cgctcaacgt
    32401 tgcaccttcc agcgctggcg tgatcatttc agcgctggcc gacgaagagg tgctggtatc
    32461 aagcgtcact tctacttcca gtggtttgcc gttacgcagc aggccaagct tcactttcgt
    32521 gcccggctct gtagtcgcga tacgagagcg caactcagca aagctattca gcggtttgcc
    32581 gttgaggctg gtaataatat cgcccgcttt gacgcccgct tttgccgagc cagaacctgg
    32641 caacacttcg ctgacaaacg cgccacgctg cacgtcaagg ttgaaggctt tggcgatatc
    32701 ggcactcatc tcggtgcctt tgatgcctaa caaaccgcgt ttgatttcac caaagtcgat
    32761 aagctgctgc gccagtgttc gcgccatatt actggggatg gcaaatccaa tcccgacgct
    32821 cccgccgcca ggcgcaagga ttgcagtgtt gatgccaatt aactcaccgt taaggtttaa
    32881 tagtgcaccg ccggagttac cgcggttaat ggaagcatct gtctggataa agttttccag
    32941 accttcaaga ttcaacccgc tgcggcctaa tgcggaaaca atgccagagg tggcggtttg
    33001 cccaaggcca aatgggttac cgaccgctac ggcaaaatca ccgacgcgca atttatcgga
    33061 gtcggcaata gcgatttgcg ttaatttgct cgggttttga atttgtaaca gggcgatatc
    33121 gctctggtca tcgctaccaa tcagttttgc atcaaactcg cgcccatcat tgagctgaat
    33181 actgattttc tgtgcctgat taatcacatg gttgttggtc agcacatagc ctttactggc
    33241 gttgatgatg acaccggaac ctaaaccttc gaagggttgt gcaggttgat ccggtaaatc
    33301 atcaccaaaa aactttttga attcttccgg gattttctgt ccctgactgg ccgttccttc
    33361 cacccgtacg ctcaccactg ccggaagcac tttttccagc attggagcca gactggggag
    33421 aggggcctga tcggcaacct ggcctggaat cgacgcgacg gcctgaaatg acgccgagag
    33481 agttaacccg acacttaacg ctaatgcact caacagctgg gtttgttttt tcattattcc
    33541 tgctctcgta cctgaatgat aagaaaagag attcaaaaca gtttgatgtt attgaatttt
    33601 cagacggcta acaatgagac accagattaa atgatagtcg gggagggaga gaagaggagg
    33661 gcgcaatggc tgcgcccgaa aaataaatta gtcgcgcttc gcgccagtac gcagcaggcc
    33721 ggatgcgcct tcagaatagt cgcgaggcat ctgcaccggt gcctgatcgt tgctggcttc
    33781 agactctgcc agacgattac ggaacgggtt tgcttcagca gacagttccg gcagcaggct
    33841 gctggagctt ttcgccatgt gctgatacag ctggcgatag tcgtgcgcca tggtatccag
    33901 taattccgcg ctgcgggcaa agtggctaac cagctcttcg cgatactcgt ccagttcagc
    33961 tttattcttt tccagttcgt actgcaacgc ctgttgctgg cgtagtttac gattaccaaa
    34021 acgcatggcc acagcaccaa taatgatgcc gacgactaac ccaattagcg catattccca
    34081 ggtcatgaac atctcccgtt gtcttttgtt tccgtagggt gttggcttca ggctccatgc
    34141 ctgcggctga ttatgccact ataaccgtta attccacaga agtggaatcc cgactgcata
    34201 tcgcgtagtg tagaacggcc tttttttcgt caaccgtgaa caacggcgca ccgattattc
    34261 aaggaataac aataagatca tgcaaagcgt taccccaaca tcgcaatacc tgaaggcgct
    34321 caatgaaggc agccatcaac ccgacgacgt tcaaaaagag gccgtcagcc gcctggaaat
    34381 tatttatcaa gaactcatca atagcaggcc accagccccc aggacgagtg ggctaatggc
    34441 gcgggtcggt aagctgtggg gtaaacgcga agacacaaag catacgccag tgcgtggctt
    34501 atatatgtgg ggcggtgtag gacgcgggaa aacctggctg atggaccttt tctatcaaag
    34561 cctgccggga gagcggaaac agcgcctgca ctttcaccgt tttatgctgc gggtgcacga
    34621 agagctaact gccttacagg ggcagaccga tccgctggaa attattgccg atcgctttaa
    34681 agccgaaact gacgtgctct gttttgacga attttttgtt tctgatatta ccgacgccat
    34741 gctacttggc ggtctgatga aagccctgtt cgcccgcggc attaccctgg tagcgacgtc
    34801 aaatattccg ccggatgaac tttatcgaaa tggcctgcaa cgtgcgcgtt ttctgcctgc
    34861 aatcgatgcc attaaacagc attgtgatgt aatgaacgtg gacgctggtg ttgattatcg
    34921 actgcgtaca ctcactcagg cgcatctgtg gctttcgcca ctcaacgatg aaacccgggc
    34981 gcagatggat aaactatggt tggcgctggc gggggcgaaa cgagaaaatt caccgacgtt
    35041 agaaatcaac catcggccat tggcgacaat gggcgtcgag aaccagacgc tggcggtctc
    35101 ttttactacg ctgtgcgtcg acgcccgcag tcagcatgac tatattgcgc tctcacgtct
    35161 ctttcatacg gtcatgttgt ttgatgtacc agttatgacg cggttgatgg agagcgaagc
    35221 gcggcgcttt attgcgctgg tggatgagtt ttacgagcgc catgtcaaat tagtggtgag
    35281 tgcagaagtg ccgctgtatg aaatttatca gggcgatcgg ctgaagtttg agttccagcg
    35341 ttgcctgtca cgtctgcaag agatgcaaag cgaagagtat ctgaagcgcg agcatttggc
    35401 gggttaaaac ctgtcacaaa tcacaaaaag gggtcgatct ttgaccccga cttctctata
    35461 atcctgcgac cccacgttac aagaaagttt ttttcccaaa actttttgtg tgctggcata
    35521 ggctattcga aggggtaggt ttgccggact ttgtcgtgtg aacctcaaca attgaagacg
    35581 tttgggtgtt caccaacgtg taactattta ttgggtaagc ttttaatgaa aacttttaca
    35641 gctaaaccag aaaccgtaaa acgcgactgg tatgttgttg acgcgaccgg taaaactctg
    35701 ggccgtctgg ctactgaact ggctcgtcgc ctgcgcggta agcacaaagc ggaatacact
    35761 ccgcacgtag ataccggtga ttacatcatc gttctgaacg ctgacaaagt tgctgtaacc
    35821 ggcaacaagc gtactgacaa agtgtactat caccacaccg gccacatcgg tggtatcaaa
    35881 caagcgacct ttgaagagat gattgctcgc cgtcctgagc gtgtgattga aatcgcggtt
    35941 aaaggcatgt tgccaaaagg cccgctgggt cgtgctatgt tccgtaaact gaaagtttac
    36001 gcgggtaacg agcacaacca cgcggcacag caaccgcaag ttcttgacat ctaatcggga
    36061 ttataggcaa tggctgaaaa tcaatactac ggcactggtc gccgcaaaag ttccgcagct
    36121 cgcgttttca tcaaaccggg caacggtaaa atcgtaatca accaacgttc tctggaacag
    36181 tacttcggtc gtgaaactgc tcgcatggta gttcgtcagc cgctggaact ggtcgacatg
    36241 gttgagaaac tggacctgta catcaccgtt aaaggtggtg gtatctctgg tcaggctggt
    36301 gcgatccgtc acggtatcac ccgcgctctg atggaatacg acgagtccct gcgttctgaa
    36361 ctgcgtaaag ctggcttcgt tactcgtgac gctcgtcagg ttgaacgtaa gaaagtcggt
    36421 ctgcgtaaag cacgtcgtcg tccgcagttc tccaaacgtt aattggcttc tgctccggca
    36481 gaaaacaatt tgcgaaaaaa cccgcttcgg cgggtttttt tatggttaaa ttctgaatca
    36541 gcgtaaaaat tggaaagttg ctttttgcta ccgtctgaca gacaggcaaa acaaaaacca
    36601 catcgccaat aagggactaa gtcaactatt tcagactaaa gcgcatctct ttttccccat
    36661 ttccggcatc gactcaccac aaaggtcgca aaatctggta aactatcatc caattttctg
    36721 cccaaatgtc gggtattgct cattttttgt ttgattttcg aacaaagaga gtcgttcctt
    36781 actgggtaac acaacttctg actggccacc tggtggctgg tagcagtaaa aattctgact
    36841 atacctggag gttttcatgg ctgtcgctgc caacaaacgt tcggtaatga cgctgttttc
    36901 cggtcctact gacatctata gccatcaggt ccgcattgtg ctggctgaga aaggtgtaag
    36961 tttcgagatc gaacacgtgg aaaaggacaa tccgcctcag gatctgattg acctcaaccc
    37021 gaatcagagc gttccgaccc tggtggatcg tgagctgacc ctgtgggaat ctcgcatcat
    37081 tatggaatat ctggatgagc gtttcccgca tccgccactg atgcctgttt acccggtagc
    37141 tcgcggtgaa agccgtctgt acatgcatcg catcgaaaaa gactggtaca cgctgatgaa
    37201 caccatcatc aacggttcag cttctgaagc agatgccgca cgtaagcaac tgcgcgaaga
    37261 actgctggcg attgcgccgg tcttcggtca gaagccgtac ttcctgagcg atgagttcag
    37321 cctggtcgat tgctatcttg ctccgctgct gtggcgtctg ccgcaactgg gcatcgagtt
    37381 cagcggcccg ggtgcgaaag agctgaaagg ctatatgacc cgcgtctttg agcgtgactc
    37441 tttccttgct tctttaactg aagcagaacg tgaaatgcgt ctgggccgga gttaatctgt
    37501 atggatttgt cacagctaac accacgtcgt ccctatctgc tgcgtgcatt ctatgagtgg
    37561 ttgctggata accagctcac gccgcacttg gtggtggatg tgacgctccc tggcgtgcag
    37621 gttcctatgg aatatgcgcg tgacgggcaa atcgtactca acattgcgcc gcgtgctgtc
    37681 ggcaatctgg aactggcgaa tgatgaggta cgctttaacg cgcgctttgg cggcattccg
    37741 cgtcaggttt ctgtgccgct ggctgccgtg ctggctatct acgcccgtga aaatggcgca
    37801 ggcacgatgt ttgagcctga agctgcctac gatgaagata ccagcatcat gaatgatgaa
    37861 gaggcatcgg cagacaacga aaccgttatg tcggttattg atggcgacaa gccagatcac
    37921 gatgatgaca ctcatcctga cgatgaacct ccgcagccac cacgcggtgg tcgaccggca
    37981 ttacgcgttg tgaagtaata caaaaacagg ccctggtggc ctgttttgtc ttttaatggc
    38041 ttaataaaat agcgtgattg tggcaatcat attcaccacg aaaccgactg ccatggggat
    38101 cgctgttcgc ttcaccaccg caaaaggcga aacgcctgca attcctgacg taacgaccac
    38161 caccgcagta atcggcgaaa ccgcgcgcgc tagcgtcgtg gcaaaatgca tcggcataat
    38221 cattacaacc gctggtacat gtagtccggc tgcgatattc ggaataagac tggcaaatga
    38281 cataaacggc gcattgccag agcccatcac aatggcacaa atggcaatga ctagcgccat
    38341 aataatcatc acgccaatac cgcccagacc agaatgctcc gcacccctga taaccgcatc
    38401 aacagtgcca atcgtcgtta agcctttcgc aaatatttca cccgcgacca ccagcgttac
    38461 cacgttggca aactgcgtac ccatgccgtc aaaaaacgcc tgcacatcgt ccattgtttc
    38521 gcgcaagtta tgcttgcgga agaactcaac aaacatcgtc accgtcaaac tcagtaacat
    38581 caccgccacc agatgcagtt ctgactgcat cagcccgatg tgggcgagga acagcgagcc
    38641 gagcatcaag attaacggca tcacaggtaa aatggcgtaa tagagcggcg ggacattatc
    38701 gagagctttt agctctgcct gttcgtgatt gatattttta tcttttttgt caaaagcgcg
    38761 ttgcacgaaa aagtgggaga tcgccaccga gataatgacg caagaggcga ccggaagctg
    38821 gtagtggaag aagtaagtgg caattttcat tcccgcgacc tgggctgcaa aaatggagtt
    38881 cgtttccaga atcccccact caatggacat cgtggttgcg ataaccgcca ccgcagagag
    38941 gcgacttact cccagactca ccagcgtcgg aaataaggtg accatcagca acatacccag
    39001 gccggaggcg ctggtaataa actgcgccat gatttggccg atgacgtaag ttgccgacag
    39061 aataatatac ggcgagcgaa tgagttttaa cgggcggctt agcaagctca ccatcgcgcg
    39121 actggctcct gtgcgctcca tgtagcgggc ataaccgccc actgccataa tcgacagccc
    39181 caatccggca atgcggttgg acagcatgcg caggatttca ttataaatat cgacgatcaa
    39241 gtagccgctg cttttggcgg gatcgagtac accaccgaac cctaaccaga caccgcacat
    39301 catcaggaag atacctgcgg cagccagcac cacctgaggt ttgtagtttt tcaggatcaa
    39361 atagcccatc gtaattaata cgatgacaga tataattatg ccgaacattg ttgaactccg
    39421 tgtcaaaaga aaacggtcaa tcccataaac ggcagattga aaacaatgat gttatatttt
    39481 ttgcaagtct atttatggtg cggatgtcgt gtttttaatt gtaggtgagg tgatttttca
    39541 ttaaaaaata tgcgcttatg attattttgt aagaacacat tcataatatt cataatgctc
    39601 gtgaatagtc ttataaataa
//
LOCUS       DAZGLJ010000021        39205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000021 DAZGLJ010000000
VERSION     DAZGLJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.021"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(135..326)
                     /gene="yfgG"
                     /locus_tag="ACHK49_000987"
     CDS             complement(135..326)
                     /gene="yfgG"
                     /locus_tag="ACHK49_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO9261674.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            344..496
                     /locus_tag="ACHK49_000988"
     CDS             344..496
                     /locus_tag="ACHK49_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9261675.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            678..2921
                     /gene="pdeF"
                     /locus_tag="ACHK49_000989"
     CDS             678..2921
                     /gene="pdeF"
                     /locus_tag="ACHK49_000989"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO9261676.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2960..4501)
                     /gene="ppx"
                     /locus_tag="ACHK49_000990"
     CDS             complement(2960..4501)
                     /gene="ppx"
                     /locus_tag="ACHK49_000990"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO9261677.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4506..6572)
                     /gene="ppk1"
                     /locus_tag="ACHK49_000991"
     CDS             complement(4506..6572)
                     /gene="ppk1"
                     /locus_tag="ACHK49_000991"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO9261678.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANVQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6743..7381)
                     /gene="purN"
                     /locus_tag="ACHK49_000992"
     CDS             complement(6743..7381)
                     /gene="purN"
                     /locus_tag="ACHK49_000992"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO9261679.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7381..8418)
                     /gene="purM"
                     /locus_tag="ACHK49_000993"
     CDS             complement(7381..8418)
                     /gene="purM"
                     /locus_tag="ACHK49_000993"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO9261680.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTQLDGKPL
                     ADYLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8743..9369
                     /gene="upp"
                     /locus_tag="ACHK49_000994"
     CDS             8743..9369
                     /gene="upp"
                     /locus_tag="ACHK49_000994"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO9261681.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9455..10744
                     /gene="uraA"
                     /locus_tag="ACHK49_000995"
     CDS             9455..10744
                     /gene="uraA"
                     /locus_tag="ACHK49_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO9261682.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHHSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10839..11540
                     /gene="hda"
                     /locus_tag="ACHK49_000996"
     CDS             10839..11540
                     /gene="hda"
                     /locus_tag="ACHK49_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO9261683.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11678..12037)
                     /gene="arsC"
                     /locus_tag="ACHK49_000997"
     CDS             complement(11678..12037)
                     /gene="arsC"
                     /locus_tag="ACHK49_000997"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO9261684.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(12058..13521)
                     /gene="bepA"
                     /locus_tag="ACHK49_000998"
     CDS             complement(12058..13521)
                     /gene="bepA"
                     /locus_tag="ACHK49_000998"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO9261685.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13734..14795
                     /gene="yfgO"
                     /locus_tag="ACHK49_000999"
     CDS             13734..14795
                     /gene="yfgO"
                     /locus_tag="ACHK49_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO9261686.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14833..15681)
                     /gene="focB"
                     /locus_tag="ACHK49_001000"
     CDS             complement(14833..15681)
                     /gene="focB"
                     /locus_tag="ACHK49_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO9261687.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(15703..17715)
                     /gene="hyfR"
                     /locus_tag="ACHK49_001001"
     CDS             complement(15703..17715)
                     /gene="hyfR"
                     /locus_tag="ACHK49_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO9261688.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17745..18158)
                     /gene="hyfJ"
                     /locus_tag="ACHK49_001002"
     CDS             complement(17745..18158)
                     /gene="hyfJ"
                     /locus_tag="ACHK49_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO9261689.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(18151..18908)
                     /gene="hyfI"
                     /locus_tag="ACHK49_001003"
                     /pseudo
     CDS             complement(18151..18908)
                     /gene="hyfI"
                     /locus_tag="ACHK49_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(18905..19450)
                     /gene="hyfH"
                     /locus_tag="ACHK49_001004"
     CDS             complement(18905..19450)
                     /gene="hyfH"
                     /locus_tag="ACHK49_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFO9261690.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(19460..21127)
                     /gene="hyfG"
                     /locus_tag="ACHK49_001005"
     CDS             complement(19460..21127)
                     /gene="hyfG"
                     /locus_tag="ACHK49_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO9261691.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDHRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDAQVIPVGPLHITSDELGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(21117..22697)
                     /gene="hyfF"
                     /locus_tag="ACHK49_001006"
     CDS             complement(21117..22697)
                     /gene="hyfF"
                     /locus_tag="ACHK49_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO9261692.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22702..23352)
                     /gene="hyfE"
                     /locus_tag="ACHK49_001007"
     CDS             complement(22702..23352)
                     /gene="hyfE"
                     /locus_tag="ACHK49_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO9261693.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(23364..24803)
                     /gene="hyfD"
                     /locus_tag="ACHK49_001008"
     CDS             complement(23364..24803)
                     /gene="hyfD"
                     /locus_tag="ACHK49_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFO9261694.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLLFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASKVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(24820..25767)
                     /gene="hyfC"
                     /locus_tag="ACHK49_001009"
     CDS             complement(24820..25767)
                     /gene="hyfC"
                     /locus_tag="ACHK49_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO9261695.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMASLFVALFLSFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25778..27796)
                     /gene="hyfB"
                     /locus_tag="ACHK49_001010"
     CDS             complement(25778..27796)
                     /gene="hyfB"
                     /locus_tag="ACHK49_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFO9261696.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADFTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGFAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27796..28413)
                     /gene="hyfA"
                     /locus_tag="ACHK49_001011"
     CDS             complement(27796..28413)
                     /gene="hyfA"
                     /locus_tag="ACHK49_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO9261697.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(28666..29136)
                     /gene="bcp"
                     /locus_tag="ACHK49_001012"
     CDS             complement(28666..29136)
                     /gene="bcp"
                     /locus_tag="ACHK49_001012"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO9261698.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(29136..29708)
                     /gene="gcvR"
                     /locus_tag="ACHK49_001013"
     CDS             complement(29136..29708)
                     /gene="gcvR"
                     /locus_tag="ACHK49_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO9261699.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29854..30732
                     /gene="dapA"
                     /locus_tag="ACHK49_001014"
     CDS             29854..30732
                     /gene="dapA"
                     /locus_tag="ACHK49_001014"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO9261700.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30749..31783
                     /gene="bamC"
                     /locus_tag="ACHK49_001015"
     CDS             30749..31783
                     /gene="bamC"
                     /locus_tag="ACHK49_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO9261701.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31996..32709
                     /gene="purC"
                     /locus_tag="ACHK49_001016"
     CDS             31996..32709
                     /gene="purC"
                     /locus_tag="ACHK49_001016"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO9261702.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32863..33090
                     /gene="vapB"
                     /locus_tag="ACHK49_001017"
     CDS             32863..33090
                     /gene="vapB"
                     /locus_tag="ACHK49_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFO9261703.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            33090..33488
                     /locus_tag="ACHK49_001018"
     CDS             33090..33488
                     /locus_tag="ACHK49_001018"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO9261704.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            33635..34498
                     /gene="ypfJ"
                     /locus_tag="ACHK49_001019"
     CDS             33635..34498
                     /gene="ypfJ"
                     /locus_tag="ACHK49_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO9261705.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            34513..36528
                     /gene="tmcA"
                     /locus_tag="ACHK49_001020"
     CDS             34513..36528
                     /gene="tmcA"
                     /locus_tag="ACHK49_001020"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO9261706.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            36602..37300
                     /gene="ypfH"
                     /locus_tag="ACHK49_001021"
     CDS             36602..37300
                     /gene="ypfH"
                     /locus_tag="ACHK49_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO9261707.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(37410..37610)
                     /gene="ypfN"
                     /locus_tag="ACHK49_001022"
     CDS             complement(37410..37610)
                     /gene="ypfN"
                     /locus_tag="ACHK49_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO9261708.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(37638..38765)
                     /gene="dapE"
                     /locus_tag="ACHK49_001023"
     CDS             complement(37638..38765)
                     /gene="dapE"
                     /locus_tag="ACHK49_001023"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO9261709.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAANLQLLARMYQRIMEQLVA"
     gene            complement(38769..39125)
                     /gene="yffB"
                     /locus_tag="ACHK49_001024"
     CDS             complement(38769..39125)
                     /gene="yffB"
                     /locus_tag="ACHK49_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO9261710.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9597 a        10034 c        10298 g         9276 t
ORIGIN      
        1 caggataaac atcagtcagg acaaagacgt aaaatttaag atgaaagaat atctgccgct
       61 ttgataattc caaagagcaa aagcatccga cagcgaagtg ccgttaggtt ctgtgaaagt
      121 ggagaaggta accgctaacg ttgtaccggt gattcgacgg agagtgtgga ttgctgagct
      181 tcttttttgc tctgatggtg ctgccaggca ccgacggacg agtagataaa acggccaaag
      241 aagaagataa agctgatgag cagtacgata cgggtcatgc gactgttaaa tcggtgtcgt
      301 tttcgcatac tggttgcctg actcacaaaa ggttccttga agtatgtccc acgctgtgga
      361 cggtacttac attaaggcac aacaggacaa aatggtcaat tcttcgttat gtaaaaaagc
      421 gtcagtggat acatatttta atgttatgga agttaattta attatttaca atgatgatgt
      481 aataatgatg aattaacatg ataaacgtaa taattcatta atcagggtta ttttgtttga
      541 tatatatcaa ttggaacttt catatgacac ttagaatcaa tgctctctat agtgatgaat
      601 aatcatcatt cgaagtcagg tgggatgcct gtctgaatac acctccttca ggatgtgggg
      661 gattctgctg agcatctatg aaactgaatg caacttatat aaaaatacgt gataaatggt
      721 gggggcttcc gctgttcctg ccttctttaa tcttgcccat tttcgcccac attaatactt
      781 tcgcgcatat ttcttccggt gaggtttttc tcttttatct gcctttggca ctgatgatca
      841 gcatgatgat gtttttcagc tgggcggcat tgccagggat caccttaggg atttttgtcc
      901 gcaaatatgc agagctgggt ttttacgaaa cgctctcatt aacggctaat tttattatca
      961 ttatcattct ctgttggggc ggttacaggg tctttactcc ccggcgtaac aacgtttcac
     1021 atggtgatac ccgtttaatt tcccagcgta tattctggca gattgtgttt cctgcaacgc
     1081 tgtttctgat acttttccag tttgctgcat ttgtaggatt actggcgagc agagaaaatc
     1141 tggtcggtgt catgcctttt aacctcggga ctttaatcaa ttatcaggcc ttactggtgg
     1201 gtaatctgat cggtgtcccg ctgtgctact tcatcattcg ggtagtgcga aatccatttt
     1261 atttacgtag ctattattcg caattaaaac agcaggttga tgccaaagtc accaaaaaag
     1321 agttcgcgct ctggctactg gcattaggtg ctttattgtt gctgttatgc atgccgttaa
     1381 atgaaaaaag cacaattttt agcaccaatt ataccttgtc attattgctg cccctgatga
     1441 tgtggggagc gatgcgctat ggttataagc tgatttcgct gctctgggcg gtcgtgttga
     1501 tgatcagcat ccacagctat caaaattaca ttcccattta tcctggctat accacgcagc
     1561 tgaccataac ctcctccagt tatctggtat tctcttttat tgtcaattat atggctgtac
     1621 tggcaactcg tcagcgagcg gtagtcagac gcattcagcg gcttgcgtat gtggacccgg
     1681 tggttcatct gccaaatgtt cgcgccctga atcgcgcgtt gcgtgatgcc ccctggtctg
     1741 cgctttgtta tttacgcatc cctggcatgg aaatgctggt taagaactat ggcatcatgc
     1801 tgcggattca atacaagcaa aaactttctc actggctgtc acccttgctg gaaccgggtg
     1861 aagatgttta tcagctttcg ggtaacgatc tcgcgctgcg gctgaataca gaatcgcacc
     1921 aggagcgcat taccgcactg gatagccatc tcaagcaatt tcgtttcttt tgggatggca
     1981 tgccgatgca accgcagatt ggcgtcagtt actgctatgt gcgctcgcca gtgaatcata
     2041 tctacctgct gctgggagag ctaaatacgg tcgccgaact ttccatcgtg accaacgccc
     2101 cggaaaatat gcagcgtcgc ggggcaatgt atttgcaacg cgaattgaaa gataaagtcg
     2161 cgatgatgaa tcgactacag caggcgctgg aacacaacca ttttttcctg atggcccagc
     2221 cgattaccgg tatgcgtggt gatgtttacc atgaaattct tctgcgcatg aaaggtgaga
     2281 atgatgaact gatcggcccc gatagcttct tgccggtcgc gcacgaattt ggtttatcgt
     2341 cgagtatcga catgtgggtc attgagcata cgctgcaatt tatggctgaa aacagagcga
     2401 agatgcccgc tcaccgtttt gctattaatc tgtctccaac ctcggtatgt caggctcgtt
     2461 ttcctgttga agtcagtcag ttgctggata aatatcagat tgaagcgtgg caacttattt
     2521 ttgaagtcac cgaaagtaat gctctgacca atgttaagca ggcgcaaatc accttgcagc
     2581 atcttcagga attaggctgc cagattgcga ttgatgattt cggcaccggc tacgccagct
     2641 atgcgcggct taaaaatgtg aatgccgatc tgcttaaaat tgacggcagt tttatccgca
     2701 atattgtgtc aaatagtctg gattatcaga tagtggcatc gatttgccac ctggcgcgaa
     2761 tgaagaaaat gcgggtagtg gcagagtacg ttgaaaacga agagatccgc gaggcggtgc
     2821 tctctttggg gatcgattat atgcagggtt atcttattgg taagccgcaa ccgttaattg
     2881 atacgctgaa tgaaatcgaa cccattcgcg aaagtgcctg aataatgcgg gccgacattt
     2941 ctcgtcggcc cgcaaagcat taagcggcga tttcaggtgt actttcttct tcaattttca
     3001 accgccagcc agccacgcct tcccagtatt cttgctcctt ttccagatca agcagtacca
     3061 gcgcattctg actaaaccag tcatgcggga aacgcagtgt ccagtgactg tcatcagtaa
     3121 tcagtgtcaa tgttggcggt gtggtggttg cctgacgttg attgtttagt aatacgccaa
     3181 ggcgcaatag ctgtatcagt ggcaggaact gtttcttctt aaacaaggta aagcgcggca
     3241 gatcgtcgag cttaatcgct ttacggtgat agcgcaccag tgtcgccatc atcagctgct
     3301 gttcctgatt aaaacccggc aagtcactgt tttgcagaat ataagcggag tggcgatgca
     3361 aaccgctgtg gttgatattc aacccgacct catgcagcat ggcggcccat cgcagtagcg
     3421 cctccagttg cggatgcgcc agcttcggtt gctgttcccg ccactgttcg tacatttgca
     3481 tagtggtatc cagcactcgt cgggcctgtt cgctgtcgat gtgatactgg ttggcgaggc
     3541 tgctggcggt gcgactacgc acatcctgat gacggaaacg tccttccatt tcatacagta
     3601 cgccttcgcg taacgccccg tcagaaaggc gcagttcacg gatggctaaa gcatcaaaca
     3661 caccacataa aatcgccagt ccaggaacga agactgtttt ccgctcttcg gaaagacccg
     3721 gtaaactcag cgatgcgaaa ttacggtgac gtaaaacttc ttttaccagt ttttccagac
     3781 gttccggggt aattatcccg tctttctcgc ccatttccat taatacttca tgggcggctt
     3841 ttatggtacc ggaagcgccc attgccacgt tccagccctg aatacggaat tgccaggtta
     3901 aagtttccag tttttgtgct gccgccatgc gagcgcgctg aaaattctct ttattgatga
     3961 ccccgccagg gaaataaagc tgggcaaagc tgacacaacc catccggcgg ctttcaacga
     4021 gaataggttc gaaattttca ccaatcacca gttccgtaga tccgccgcca atatcaataa
     4081 ccagtttgcg acctttttcc ggttgggtat gttccacgcc cataaaaatc agacgggctt
     4141 cttcattacc ggaaataatt tcaatcgggt agggaatgac cttttccgcg cgtttcagaa
     4201 agtcagtggc gttcagcgcc tgacgcagtg tatgggtgcc aactatacag acgctggcag
     4261 gagaaaaccc ttgtagccgt tcggcaaaca gcgacagaca gttcaaaccg cgcgtcattg
     4321 cctcttcact caacatatta tctggcccca ggccgtccgc cagatgcacc cgctgtttca
     4381 ggcggccaat aatctgcatg gcaccatcta ccacacgggc tatgaccatg tgaaaactgt
     4441 ttgaaccaag atcgaccgca gcaaactcct gcggacgagg ggatttatcg tgtattggca
     4501 tagggttatt caggttgttc gagtgatttg atgtagtcat aaatcgccaa ctgcgcccgt
     4561 actttgcggc gattgccgcg gggaacgtag cgattactga gttctttatc gatataacgt
     4621 gctttgaccg tatcgctgaa caatatgtcg atgatgtcca gcacccgctg cttcaggcgc
     4681 ggatcgagca gcggtgtcgc cacttcaata cgataatcaa tattgcgcgt catccagtcg
     4741 gcggaagaaa ggtagacctt tttatcgccg ccattttcaa aaatataaac ccggtcatgt
     4801 tcaaggtaac ggtcaacaat actgatggca cgaatgttgt cgctaatgcc ttccagattg
     4861 gggatcagcg aacacattcc gcgaaccagc agattaaccg gtacgccgga gctggaggcc
     4921 gcatacagac gatcaaccag gcctttatcg acaaggttat ttagcttcag ggtgatacca
     4981 ctgggcagcc cttgctgcac gttggcgatt tcgcggtcta ccatttcata taacagacgg
     5041 cgagagtttt gcggtgacac cattaaataa tcaaatgtca ctgggcggta tgggttttca
     5101 ataaagttaa atacccgccg tacttcgttg gtgatacgcg catctgcggt cagcaacgaa
     5161 tagtcagtat aaagacgcgc ggttttttcg ttaaagttcc cggtcccgat gtgtgcgtaa
     5221 cggaccactt caccgttttc tttacgtgaa atcaggaaca gtttggcgtg aattttcagc
     5281 cccggcgcag agaagataac gtgcacgcct gcttcggtca ggcgcttcgc ccagtgaatg
     5341 ttggcttctt cgtcgaaacg cgcctgtaac tcaaccacca cagtgacttt cttaccgtta
     5401 tgtgcggcgt ggatcatcga gtcgatgatg cgtgaatctt tcgccacgcg gtaaatgtta
     5461 attttaatcg ccagtacgct cgggtcgaac gaagcctgac gcagcagttc cagcacatgc
     5521 tcaaaggtgt gataaggata atagagcaac acatcgcgtt cgcgaatggc atcaaaacca
     5581 ttgcggaact gggctttatc aaaccaaatg tggcgtaaac gcggcagtgg tttgttcacc
     5641 agattggctt tgccgacatt ggggaaatta ataaagtctt taaaattatg ataacgaccg
     5701 ccggggacga tggagtcgta gcgggaaata gtcagttttt cgcgtaacac ttcaaccagc
     5761 gcattgggca tatcgcgctg ataaacaaaa cgcaccggct cagcagttaa acgctgcttg
     5821 agactggaag acatcaactc catcaggctg gcttccatct catgcactaa atcgtattcg
     5881 gcatcgcgtg tcatcttcat tgaataggca ttcagcgcgt cataatcaaa gaagcctttg
     5941 aaaatatcat caaggcagta acgcagaatg ttatccagaa gaatcatcgg cttgcgtcga
     6001 cgcggcgctt ctggcggtaa attcacaaag cgcggcactt tatctgatgg gatctccagc
     6061 agcgcgtaac ggatggtatc accacggata atttccaccg ccagataggt gtaatcatcc
     6121 ttcaggaact gcactaagtc agtgtcagga ttgattaaaa tcggcgtaat gtgctgacgc
     6181 agatactgct taaaataatg acgaagccag ttttgttgat tgacggagag ctggcgttca
     6241 ttaatcagga agatctggtt gcgcgccatc tccagcaata gctcgttgta gaggccgtcg
     6301 aattcctgat cggctttcag cacccgggac tgaattttgc ccagtaaatg gcgggaatga
     6361 gagttggagc cttgttcttc gctaataatg atgcgtcgct tcagttcagc gaagcggact
     6421 ttatagaact catcaaggtt attggaatag atccccagga aacgcatcct ttcaatcagc
     6481 gggttagatt tgtccgccgc ttcctgaagc acgcgttcat tgaacgataa ccaactgagc
     6541 tctttttcga tgtatagctt ttcctgaccc attaccactt ttactccgtt ttattcacgg
     6601 gacactgcct ggatattatg gcgagcaata ttggcagatg tccaactatg ccagaaaagg
     6661 gggaaaaaaa ctgctggagc ttgcgattta caggtgaatt gaaaaacgcc agcggcagag
     6721 ctggcgcttt aactacgggg cattactcgt cggcagcgta gccctgcggc ggcagacgtt
     6781 gaccatccag ccacgcggcg ttttcgtgca ttttcagacg accatcggca aaccagctaa
     6841 tcaccagtgg ataaatagcg tgttcctggg tttgcacgcg ggcggtgatg tcatcttccg
     6901 tatcaccagc aaataccggg actttcgcct gtaaaataac cgggccaccg tccagttcat
     6961 cggtgacgaa atgcaccgat gtaccgtgct cttcatcacc attttccagc gcttgacgat
     7021 gggtgtgtaa tccgggatat ttcggcagca gagaagggtg aatgttcagc aaacgcccgg
     7081 catagtggga gacaaacgcc gggctgagaa tgcgcataaa accagccagc acgaccacat
     7141 cgggtgcgta catgtcgatt tcatgaatca actcccggtc ataggcttca cgactgtcaa
     7201 acgcgctggc gatgagcgta tgcgttgcaa tacccgcctg gcgggcgcgt tcaaggccga
     7261 acgcgtcggc cttattgctg aaaactgccc gtacggtgcc tttaattttg ttggttttac
     7321 aggcgtcaat aattgcctgt aaattacttc cgttaccgga aataagcacc acaatattca
     7381 ttattcgata accacgcgtt gttcggaatc agaggctttg atgataccga ttttccacgc
     7441 gttttcaccg ttggcattga gcagggcgag ggctttgtcc acttccggag caggcagggc
     7501 aataatcatc ccgacgccgc agttgaaggt gcgatacatt tcatggcgct caacgttacc
     7561 tgccgtttgc agccagttga acacttccgg ccactgccag gaagattcat caatcactgc
     7621 ctgagtatta tctggcaata cgcgtggaat gttttcccag aagccgccgc cggtcaggtg
     7681 cgcaatggca tgcacatcga ccttttcaat caactccagc actgacttca cgtaaatgcg
     7741 ggtcggtgcc agcagataat cggctaatgg cttaccatca agttgggtgg tttgcggatc
     7801 acaaccgctg acttcaagaa ttttgcgcac cagcgaatag ccgttcgagt gcggaccgct
     7861 ggaaccgagt gcaatcagca catcgccgtc gctgacttta gagccgtcga tgatttctga
     7921 tttttctacc acgccaacgc agaaacccgc gacatcgtaa tcctcaccgt gatacatccc
     7981 cggcatttct gccgtttcgc cacccaccag tgaacagccc gattgcagac atccttccgc
     8041 gataccgctg atcaccgctg aagcggtatc aacatccagt tttccggttg cgtaatagtc
     8101 gaggaaaaac agcggctctg caccttgcac caccaggtca ttaacgcaca tggcgaccag
     8161 atcaatacca atggtgtcgt gacgttttaa gtccattgcc agacgcagct tggtacctac
     8221 gccgtcagtg ccggaaacca gcacgggttc acgatatttt tgcggcaatg cacacagcgc
     8281 accgaagccg cccagaccgc ccatcacttc cggacgacgc gttttcttca ctacgccttt
     8341 gattcttcca accagagcat tacccgcgtc aatatcaaca ccggcatctt tgtagctaag
     8401 agaggtttta tcggtcactg cttgggtccc cacgcgttac ttgcggtaga aaaataaaat
     8461 tcggcgcaat tctaacaggg aaagcaaacg tttgcgagac tgctttacac aacctttttg
     8521 cacgtctttt gcccaggcgc gcggcgaaag aagacttgta ccagggtaaa ggttagtttt
     8581 cggatggaat aatcttcttt cataaccatt tgaatataaa ataactttat ctcaagccgt
     8641 tatcattttg actaaagtca acgaaaagaa tattgccgcc ttgaagaaag gaggtataat
     8701 ccgtcgattt tttttgtggc tgcccctcaa aggagaaaga gtatgaagat cgtggaagtc
     8761 aaacacccac tcgtcaaaca caagctggga ctgatgcgtg agcaagatat cagcaccaag
     8821 cgctttcgcg aactcgcttc cgaagtgggt agcctgctga cttacgaagc gaccgccgac
     8881 ctcgaaacgg aaaaagtaac tatcgaaggc tggaacggcc cggtagaaat cgaccagatc
     8941 aaaggtaaga aaattaccgt tgtgccaatt ctgcgtgccg gtctggggat gatggacggt
     9001 gtgctggaaa atgttccgag cgcgcgcatc agcgttgtcg gtatgtaccg taatgaagaa
     9061 acgctggagc cggtaccgta cttccagaaa ctggtttcta acatcgatga gcgtatggcg
     9121 ctgatcgttg acccaatgct ggcaaccggt ggttccgtta tcgcgaccat cgacctgctg
     9181 aaaaaagcgg gctgcagcag catcaaagtt ctggtgctgg tagctgcgcc agaaggtatc
     9241 gctgcgctgg aaaaagcgca cccggacgtc gaactgtata ccgcatcgat tgatcaggga
     9301 ctgaacgagc acggatacat tattccgggc ctcggcgatg ccggtgacaa aatctttggt
     9361 acgaaataaa gaataaaaat aattaaagcc gactttaaga gtcggctttt ttttgagtaa
     9421 agcgcctata acacataata cagaggataa tactatgacg cgccgtgcta tcggggtgag
     9481 tgaaagaccg ccacttttac agacaatccc gcttagtttg caacatttgt tcgccatgtt
     9541 tggtgcaacc gtcctggtgc ccgtcttatt tcatattaac ccggcgactg tactgttatt
     9601 taacggtatt ggaacgctgc tgtatctctt catctgtaaa gggaaaattc cggcttatct
     9661 tggttccagc tttgccttta tttcaccggt attgttactg ttgccgttag ggtatgaagt
     9721 cgcgctgggc ggctttatta tgtgcggcgt gctgttctgc ctggtttctt ttatcgtgaa
     9781 gaaagcgggg accggctggc tggacgtgct gtttccacct gcggcaatgg gcgcaatcgt
     9841 tgccgtcatc ggtctggagc tggcgggcgt agctgccggt atggcgggtt tactcccggc
     9901 tgaagggcaa acgccagact ccaaaaccat catcatctct attaccaccc tggcggtcac
     9961 ggttttaggt tccgtgctgt ttcgtggttt cctggcaatt atcccgattt taattggcgt
    10021 gctggtgggg tacgcgctct ctttcgcaat gggaattgtc gataccacgc cgattattaa
    10081 tgctcactgg tttgcgctgc caaccctcta tacgccgcgc ttcgagtggt ttgccattct
    10141 gactattctg ccagcggcgt tagtggttat tgccgaacac gtagggcacc tggtagtaac
    10201 ggctaatatc gtcaaaaaag atctgctgcg cgatccaggt ctgcaccatt cgatgtttgc
    10261 taatggcttg tcgaccgtga tttccggctt ctttggctct acgccaaata ccacttacgg
    10321 agaaaacatt ggcgtgatgg cgatcacccg tgtttacagt acctgggtta tcggcggggc
    10381 ggcgattttc gctatcctgc tttcctgcgt cggtaaactg gctgccgcta tccagatgat
    10441 cccattgccg gtgatgggcg gcgtttcgct gctgctttat ggtgtcatcg gtgcttccgg
    10501 tattcgtgtt ttgatcgaat cgaaagtgga ttacaacaaa gcacagaacc tgatcctgac
    10561 ttccgtgatt ttgatcatcg gcgtcagtgg cgcgaaggta aacatcggcg cggcggagtt
    10621 aaaaggtatg gcgctggcga ccatcgtcgg tatcggctta agtttgatct tcaaactgat
    10681 cagtgtgctg cgccctgaag aagtggtgct ggacgcagaa gacgcggata taacagacaa
    10741 ataatgtacc gaccgggcag tgttgctgcc cggttcaaac atcatgggat tctgtggtaa
    10801 acttctcgcg attttgtgaa atcctggttg aggtatctct gaacacaccg gcacagctct
    10861 ctttgccact ttatcttcct gacgacgaaa cctttgcaag tttctggccg ggggataact
    10921 cctctttact ggctgcgctg caaaacgtgc tgcgtcagga acatagcggt tacatctatc
    10981 tctgggcacg cgaaggcgcg gggcgcagcc atctgctgca cgcggcttgc gcggaattgt
    11041 cgcagcgtgg cgatgcggtg ggctatgtcc cgctggataa acgcacctgg tttgttccgg
    11101 aagtgctcga cggtatggag catttgtcgc tggtctgtat cgacaacatt gagtgtattg
    11161 caggcgatga gttgtgggag atggcgattt tcgatctcta caatcgaatt ctggaatcgg
    11221 gcaaaacacg attgttgatc accggcgatc gtccaccgcg gcagttgaat ctgggattac
    11281 cggatctcgc gtcgcgactc gactgggggc agatctacaa attgcagcca ctttctgatg
    11341 aagataagtt gcaggcgcta cagttacgcg cgcgtttgcg tggttttgaa ctgccggaag
    11401 atgtggggcg tttcttgctg aagcggctcg acagagaaat gcgcacgcta tttatgacgt
    11461 tggatcagtt ggatcgtgcg tcgattaccg cgcaacgtaa gctgaccatt ccgtttgtga
    11521 aagaaattct gaagttgtag ataaggtgtt tattgtcgga tgcggcgcga acgccttatc
    11581 cgaactacgc gaagttaatc catagtattc gtaggccgga taagacgtgg caagcgtcgc
    11641 atccggcaat gtgcttcaac tagcgatgga gcacatctta acccacgatt tccagtacct
    11701 gctccggtgg acgaccaatc cgcgctttgc cattcgcgac cacaatcggg cgctccatta
    11761 gcttcggatt atccaccatt gcctgaatca gcgcctcttc gctaagcgaa ctgtcagcca
    11821 ggttcagttc tttataaaga tcctctttct ggcgcatcaa ttcacgggcg ctattcatcc
    11881 ccagcatttt cagcaaatcg cgcagcgttg ccgcatcggc gggtgtctca aggtagagta
    11941 ccacttctgg ctccacgccg ttttctttca gcaggttcag cgtttcccgg ctctttgaac
    12001 agcgtgggtt atggtagatt tttacctgtt tggtcattgt tcttccttta atgcgaatta
    12061 catcttggta taaggcttaa agcgttcctg cagctggcgc aactggtcga tgcgcgcatc
    12121 gtaacgcgct tgttgcaggc tgcctaattt cacctgcgaa ctggcgctac tcaacagcga
    12181 aatggcctga tcgagtcgtc cggcgagcgc ataaccttct gctcgcgcag ccagctcctg
    12241 atcgcggtta tttagcgcgg cttccgcctg tgccagtaaa tcccagccgt tgctgtcatc
    12301 tttattatta aaggtgtagc gattcagaat attggccgct tcttgtggtt gaccgccttg
    12361 tagataagcg ttcgccaggt tgagctgcaa caccggattg gtgcgcaaat cacgggcatt
    12421 tttcaggcga ttgattgcct cattggcttt gttttgccca agatcgatat cggtagccag
    12481 atcgagatac catgcgttgc caggttctgc cgccagtaac ggttgcagcg ttttacgcgc
    12541 ctcgtcgtat ttattggctt ccatcgcctg taaagcacga ccatattgcg ccgctcgttg
    12601 ctgacgaacg tttcctttcg cccattcatc cagcaaatca ctggtgagct ggttacgtcc
    12661 ggaattatac atccccagtg tgcgcgcttt cgccagatag aaatcttccg acgactgcac
    12721 caccatcggt cgcatctgat tagcacggtt gcgggcatct gccagacgac tttccggcaa
    12781 cgggtgagtc aataaaattt ccggcgggcg cgaggagtaa cgcgcctgat cgagtaattt
    12841 ttcgaggaag gttggcatcg cctgcggatc gaatcccgag cgttgcagca cctgaatacc
    12901 aatgcggtcc gcttcctgtt cattttgctg ggtgaaactg atcattccct gacgtgttcc
    12961 cgccagtgta ccggtcagcg ccgccatccc cgcctgcgga ctggccatcg ccagtaaaat
    13021 agaacctaac gcgccgaccc aggtcagcgg cgcgctgcgt tgttgatctt ccatcgctcg
    13081 cgccaggtga cgttgggtga cgtgggagat ttcgtgcgcc ataactgaag ccagttgact
    13141 ttcgttatcg gaataacgga acagggcaga gtgcagcacc acgttgccgc caaagaaagc
    13201 aaaggcgtta atttcgtcgt tgttgatcag aaaaaaatga aacggtgtct taaccgaatt
    13261 ggcatgcgaa accagacgca tccccagcga attaatatat tgcgttaaca gcgggtcatt
    13321 aattaacggc gcgctgccgc gtagctggcg gacataatag tcgcccatct gcatttcctg
    13381 accaatggaa agcgtgcttc ctgcggaggt tcccatatcc ggcaaggtgt ctgcgctgtc
    13441 ggcaaacgcc ggggctacct gaccaatggt catagcagca atgagggttg caaccaggtt
    13501 ttttttcaac tgcctgaaca taacctctat cctgtatttc tgaagatagt catttgaccg
    13561 atactgttaa atatcgttcc caccgcgccg cctttgagtg taacggcgaa gattagccca
    13621 ctcaaatgac ttttcagttt cctgaatccg tcataaaaag cgaagtcttt tttgtgacat
    13681 ttcgatacaa ttgcggatcg caatcccgct cttgaggttc agggaaggtt tttatgctcg
    13741 aaatgttgat gcaatggtat cgccgccgtt ttagcgaccc ggaagcgatt gccttgctgg
    13801 ttattttagt tgccggattt ggcattatct ttttctttag tggcctgctt gctccgttgc
    13861 tggtggctat tgtgctggcc tatttgctgg aatggccaac cgtgcgcctg caatctattg
    13921 gctgctcccg ccgctgggcg acgtcgattg tattggtggt ttttgtcggt atattgctac
    13981 tgatggcgtt cgtggtactg cctatcgcct ggcaacaggg catctactta atccgcgata
    14041 tgccggggat gctcaataag ctttctgact ttgccgccac gttgccgcgc cgctatccgg
    14101 cgttaatgga cgcgggcatt attgatgcaa tggccgaaaa tatgcgcagt cggatgctga
    14161 ccatgggcga ttcggtggtg aaaatttccc tcgcctcgct ggtcggtttg ctgaccattg
    14221 ccgtctatct ggtgctggtg ccattgatgg tcttcttcct gctgaaagac aaagagcaga
    14281 tgctgaacgc cgttcgtcgg gtgctgccgc gtaaccgtgg gctggcagga caggtgtgga
    14341 aggagatgaa tcaacaaatc accaactata tccgcggcaa agtgctggag atgatcgtgg
    14401 tagggatcgc cacctggctg gggttcttgc tcttcgggct gaactattcg ctgctgctgg
    14461 cggtgctggt cggcttctcg gttcttattc cgtacattgg cgcatttgtg gtgaccattc
    14521 cggtggttgg cgtggcgcta ttccagtttg gcgcaggcac ggaattctgg agctgcttcg
    14581 cggtgtatct gattattcag gcgctggacg gcaacctgct agtaccggtg ttgttctccg
    14641 aagcggttaa cctgcatccg ctggtgatta ttttatcggt ggtgatcttc ggtggtttgt
    14701 ggggattctg gggcgtattc ttcgccattc cattggcgac gctgatcaaa gccgtgatcc
    14761 acgcctggcc cgatgggcaa atcgcgcaag aataatcttc agcccgacga actgtttcgc
    14821 cgggctgatt tttcaggatt cctgacgtaa ataaatagcc cgataacaca tactcaccag
    14881 cactgcaccg ccgataatat tacccagcat caccgggagc agattggcgg taataaaatg
    14941 gctgaccgtc agtgccggaa aattgtctgc gctactgtgc gccagttgcc agaaggaggt
    15001 aggggcgaaa tggcgaatgg caatggcgaa cggaatcaca aacaaattgg cgatacagtg
    15061 ctcaaagcca ctggcgacaa acagggtgat gggcaaaatc atggcgacga ttttgtcgca
    15121 taacgaacgc ccgcaataac tcatccacag cgccaggcaa accatcagat tgcacataat
    15181 gccgaggctg acagattcag taaatgtatg atgcattttg ccctcggcgc agtgtaaaac
    15241 cgcgactccc cacatcgcgt tttcactcat caccagcccg gaaaaccaga ttaacaaact
    15301 gaaacaggca atacctgcca gattaccgca ggccaccaga agtgcgttaa tcagccaagt
    15361 tcgccaacta ataacgcccc tactttttgc cattaccgtc attaccgacg aggtgaacag
    15421 gttggtgccg caaaccgcca gcaaaataaa gccaagtgta aagcaaaggc cgcccaccag
    15481 atgggttaat gcctgtgaag acggggcatc ggcgataact gaaaggtaaa aagtaaatcc
    15541 gatcgccata aacacgccag cggacatcgc cattaaaaag accgacactt tactgcgggc
    15601 aattttatgg gcggcaatcc gttcagcgat tgccgctttt ctggcgctca actgcaagtc
    15661 gaaagagagt ttgtttctca tgcatactcc tgcaaaagca gattacaaca cctcgcgaac
    15721 cgagattccc agacgctgca ttcgtgacag cagcgtggtg cgcttcatcc ctaatcgtgt
    15781 cgccgcgcca cggggcccgg caacaatgcc attggtttct cgcaatacct gaacaatgcg
    15841 ctgacgttct tcttcgtcat tctctggcgt cgtcgggtgc agcaactgcg ccattgaact
    15901 gcgaagcgct gaatcttcat ttagcgtcgg cagtaaacgg ctttgtcgga cattaagatg
    15961 taaattcaga ctgttaccgc gagtcagcag taccgctcgc tcaatcacgt tttccagctc
    16021 gcgcacgttg cccggccagt cccacgacat caactggcgt agcgcctcgg tcgggatggc
    16081 gtcaattgaa cgattcatat ggcgcgccat tttttgcgtg aaatgctttg ctaaaagagg
    16141 gatatcttcc ggacggtcgc gcagcggcgg caattccagt gggaagacat tcaggcgata
    16201 aaagagatcg ctgcgaaact ggcgatcttc aaccatttgc cacaaatcac ggttggtggc
    16261 ggcaatgact ctgacattta ccgggatcgt tctactcccg ccgagacgct caatctcccg
    16321 ttcctgcaat acgcgcagca gtttaggctg aagttctaac ggcagatcgc caatttcatc
    16381 gagaaacaac gtgccgccat cggcaatttc aaaacgacca cgatgggtat taatcgcacc
    16441 agtaaacgcc cctttgtcat gaccgaataa ctcactttcc agaagactgg cggggatggc
    16501 agcgcagttg attttgacca gcggcttgtc gcgtcgcggg ctaagttgat ggatcgctct
    16561 ggcgatcacc tctttgccgg ttccggtttc accgcaaatc aacaccgtac tgtcgctctt
    16621 cgccacaata tctacctgct ggagtaggtc ttccattgcc tggctttgat agataatgtc
    16681 accgatgccc agattcgaaa ggagctgctc gctaagctgg tggttttctt gctgcaaact
    16741 ttcctgcaaa tcggtcatgc tacgccaggc atcggcattg ccaacggcaa tagcgatgcg
    16801 atcggctatg tgttgtagta gctggcagtt ttcctcacta aagagagtgg aagaggtatg
    16861 cgccagcaac aatgcacccg gtgtatggtt gccaaaggta agcggtataa ggagcgcaga
    16921 ttcgcagccg ttagatgcaa gtaagagtaa taacgggtcg cgttgccaga gaaacagatc
    16981 gtctgcacgg tgcgtcaagg tcggttgtcg ggtttgtagc gtttgtgtca gcaatacact
    17041 gtcgccaggc atattgcgtg gcagacacgc acaatgtgag gcagaaagat cgctgcacca
    17101 caggctaaac ttctcgttct ttcgatgatc gcccagtacc atactgactg aagccagacc
    17161 gaaaaaatga tggatctcac gagcgacgtc agcgatcaga tcgtcgagat caagatgtga
    17221 cagcactgca ttagtaatat caaccagtac ccgatgatga tcgcgatctt tctccagcaa
    17281 acgcagcgcc acctgctgtt cttcatcatt gagccatgat tgcagaaccg gggtgattat
    17341 ccgcgccagc cagttaaaca gtgtgcaatc ctggggagta aaacggctgc cgtgggtacg
    17401 aataaagcgt agctccgcca ttccactact gccgagaagg acggcgtgac gcgaataatc
    17461 gaccatctcg ccctgatact ctgttgttgc cgtcacatcg ctgccatcat catggtagcg
    17521 gtaatagaag ttgcttttga gaacatgcag ttcaataagt tgtaccgggc tgaacggcgg
    17581 cggcatcggg atgaaggcgc gtaataaaga cgccttaccg tgtttctgtg ctaaccgctc
    17641 cgcgagcatt ccgtttaccg cttcaattgt tattccttgt ggcggggcaa acatcgcctc
    17701 gtctgacata gccatcaaca gtccttatta atgagaaatc cttctcacgc aatttttctg
    17761 gccatcagat agatggccgg ttcctgcaca atttcatcca acagatgaat gaattgaatg
    17821 ctacgcgcct gccacacaga cgtcgcgtca tccgccagtg gcgcgaatgc ccgggtcaac
    17881 agggcggtgt ggctggcgtc aataacaatc tcaccaaaag tcatcacccc cagcatctta
    17941 cgtcgggctt gctcctcttc gacctgggca agccaatctt cgtattccgt cagtgggcag
    18001 gggaaggcga cattcagaca atcaatcacg ccaacgtgat gaccgatagc cagcgagtaa
    18061 tacattacct gagagctgtg ttccggcatc tcgtcgctac tggcgacaaa cttttttcgc
    18121 agcgtccaga aaacaatttc cccgcactct tcagtcatgt aacccccgga gtacgcgaaa
    18181 gagttgctga acgatactat tgagacgtgg atcgtcggca tcgcgcaacc aggtgttaac
    18241 ccgatttcct gtaggatcgt cgcttaaatg gcgcaggagc cgatcgcaaa tttctcgccc
    18301 ctgacgatag cccgccagcc gccgcgcttc tcgctcaatg gcgatacgct gtgatggcgg
    18361 gatctgcggc cacaacggtt gcatagtcac cccggtggga tcgcgataat ccaccgcgtg
    18421 aatcttctgt tgcagcaaac cgagcgccac ggcgaaaccg tgaatggtgg cggccggtgt
    18481 tggcgggcag ccggggatcc aaacatcaat ggggacaatg gtgtcgctgc cgccccagac
    18541 gctgtagaga tcgtggaaaa tccgccgccg gcaccgcacg cgccgtagga aacacagatt
    18601 ttatgatcgg gggcagactc atacgcccga agtgcaggca tacgcatcgc ccgggtgact
    18661 gcgcctgtaa ataacaaaat atcggcgtga cgcggtgatg aaataacctt aatgccaaaa
    18721 cgttctgcgt cgaatactgg tgtgatggcg gcaaaaattt cgatttcaca ggcgttgcag
    18781 ccgccgcaat cgacgcgata aacgtaagcc gagcgacgga tatcctgtag caaatgctgc
    18841 ttcatctttt gcgtttgctc gtccagcgtg atgggctggc tgacatgttg tgtaagcact
    18901 ggactcatag ctgctcctta gccaccagcg gtacatctgt atcgtcgttg agcagcgtcg
    18961 cgcgttgttt gcactccggg cagacgctcg cttgcgccca taacatttcg cggttttgtg
    19021 gcgcattttg ttgctgtgcc aacaattcag cagccagtgc gacggttttt tgcggggcaa
    19081 acgggcgttc gcaacggctg caacgttgta gatggaacgt cgcgcgggta tagagatcgg
    19141 ctttattggt gacggtcagt tcaaagttat tggtaagctg gatggctctg gtcgggcaca
    19201 cttcttcaca acgtccgcag taaatacaac gcctcagata gagctgccag gtgcgcgaat
    19261 tttgctggtc gtcggtctgg atagtcagcg catttgccgg acaagcacag gcgcaggcac
    19321 cgcaggcaat acattggctg ggcatcaggt ccggtttccc gcgaaagcct gggctgacct
    19381 ccagtggcgc gaagggatat ttcaccgtcg cggttccggc gcgcataata gttttcagta
    19441 acttcagcat cttctgtcct tacttcagcg gcgaacggtt acgatcaata ccgtagcgtt
    19501 cgatctcttt atacggcacg gtttttgact ggcgcttgcg cacatctacc agcgtcacac
    19561 ggtcggtaca ggagtagcag ggatcaaggc taccgataat cagcggtgcg tcagaaacgg
    19621 tattgccgcg cagcatgtaa cgcaacaccg gccagttggc gtaggtggcg gcacggcagc
    19681 gccagcggaa caatttttgg ttatcaccga gcatgctcca gtgcacgtct tcaccgcgtg
    19741 gcgcttcaac aaagcccagc gcgaatgcgt gaggtttata gctaaagcct tcggtcagca
    19801 gtggggtatc cggcatgttg tcgagggcaa attccagcat tgccagcgaa tcaaacgtct
    19861 ctttgacacg gaccatcacg cgggagaaaa catcgccgcc ggtaaaggta aacagtgttt
    19921 ttggaatatt gccgtagtcg gcgtaggggt gatcaaagcg caaatcacgg gcgatttgtc
    19981 ggtcgagaat gccaatgccc tgagtgcgtt gttccatatt cggcgtagca agcagcatct
    20041 ctaccagctc cgacacgtcg gcgcgcatct cgcgcaccag tttcagcgtt tgcagacgtt
    20101 gctctttgag aatatcgcgg cgaacaccac caatcagatt cagaccgtag gttttacgcg
    20161 acccgatcag caattccgcc atcgtcatcg acttttcccg cacgcggaaa aattgcataa
    20221 agccggtatc aaaaccaacg aaatggcagg agaggccaag gttaagcaaa tgactgtgta
    20281 gccgttcgac ttccagcaga atcgagcgaa tagtatgtgc tcgttgcggc acctcaatcc
    20341 ccagtgcatt ttcaaccgag ttggtatagg ccacactgtg ggcaaaaccg caaatcccac
    20401 acacgcggtc cgataagaag gtcacttcgt tgtagcccat ccgcgtttct gccagtttct
    20461 ccatgccgcg atggacataa aacaggcggt aatcagcatc gacaatttgc tcgccatcca
    20521 caaacaagcg gaagtgaccc agttcatcgg aggtgatatg cagcgggccg acagggatca
    20581 cctgcgcatc gctgttgccc tcattgataa acggatacgt ttcggaatca gtcgtcggtt
    20641 cagggcgcag tcgataatcc atcgcatctt tgcgcagcgg atgcatatct tccggccagt
    20701 catcgggcaa caccaggcga cgatgatccg gcaggccaac cggaatcagc ccgtacatat
    20761 cgcgaatttc tcgctcgccc cagaccgcgg cagggacgcg cggtgtgact gacggaaact
    20821 cccgactgtc ggcatcgacc agtgccttca ccacgatcca gcatttttcg gccccttcca
    20881 ttgaaagggc ataataaacc gcgtaatgac cgttaagtgt ccgctcgtcg ttgccaaaca
    20941 ggactggaag ccagccatca tgttgataat aaagataatg tacaacgtca ggcagcagat
    21001 tgattttcac cgtaatggtg acctgttcag gcgtttgtcg ctcttcatcc agcaccgcgc
    21061 cggggaactg cgttttcagg gcggcgagaa tcgcttcgcc acgatttgac gatgaattaa
    21121 cgttcactgt gtttctccga aacagatgcg gtgcctgaag gcaaaaaatc atgccaggtg
    21181 ctacgttgtg caggcaggtc gtgcgtccct gagaggacta tagtggaagc gcccgccagg
    21241 atcctgatga caggttgtgg aatatgcgtt cccatcgcca gcatcatgac cagcagaatc
    21301 accattggcg aggtggtcaa ccagccgaga tcaccccggt taacggcctg cggcggtttc
    21361 gccattaaca cccgcgctgc catccgtacc aggcccgcca gcaccagcgt taacagcaat
    21421 aacagcagga cgataatcag caggtgatta cgtgccagtc cggcggtaat ggtcataaat
    21481 tcgctaagaa aaatgttgaa gggcggcatc cctgccagcg ccagcgcacc gccgccaaac
    21541 agcacggcgg taaatggcat gattttgagc atcccacaga cgacgttgag atcgcgcgtg
    21601 ccgtacttga gcagtacatt gccggaaccg cagaacagca gcgtttttgc cagactgtgg
    21661 tttaaggtgt gcagcagcgc ggcaaaaatt cccagtggcc cgccaatgcc tagcgccacc
    21721 gcgaccagtc ccatgttctc cacgctggag tacgccagca gacgcttaat gtcccgctgt
    21781 accagaatga aaaatgccgc cacggcaacc gacaacatgc cgaagatgag caacaaccgg
    21841 ttggggaaat cgctgccgat ggcctggcag ataatgatgt aatagcgaat tagcaccaac
    21901 agcgcgcagt tcagcaatac ggcggagagc agggcgctga ccggactcgg tgcttcactg
    21961 tgagcatccg gcagccaggc gtgcatggga aatagcccgg ttttggtgcc aaagccaatt
    22021 agcacaaaca caaaggccaa cagcattaat gttgggtcaa gcaaggacga ttgcttaaga
    22081 acctcgctcc agaatatcgc catttctgcc tgcggcataa cgctggcgac gttggcgtat
    22141 accagcacgg taccgaacag accaaaagcg acaccaacag tacaaataat gatgtacttc
    22201 catgctgctt ccagcgatga acgctgaccg taaatgccta ccagaaacgc cgagcttaag
    22261 gtggtggctt cgatcgccgc ccacatcaca atcaggttat tgctggtaac aaccagcagc
    22321 atggtaaaca aaaacagatg gaagaaaccg tagtaatcgc acagcgtaac gggggaaagc
    22381 tcgccgtgtg ccacttcatg acgcatgtag ccaatcgagt aaacaccggt gagaaagcca
    22441 atcacaccaa gaatggcgag gaacaaaccg cccagaccat caatatgaag ccacagtccc
    22501 gcagcgaata tttctcctgc atcagcggca gtttggacca cccagagtgc cagaatcagc
    22561 agcagtgtga tccctaagct atgtaataca gtcaccgtac aagtcgcaga gagcccccgt
    22621 ttccggcagg caaaacagag cagcgaaaaa agcagcggcg tgagcaggag taaagcgaac
    22681 atcacagaat aactcatctc attatccctt cagcgcggtc aagttgttca cgtccagcgt
    22741 gccgtgggta cgccatattt ttcttgccag taacaccatc acaatgacgg cgaagatggc
    22801 gtcggtagcg atacctattt ccaccagttc cggtgctcgc caggcaagaa gcgccagcac
    22861 cagatgggag ccgttttcca tcaggcagta accaaaaatt tgccgcagga tattgcgctg
    22921 gctgacaatg cacagcaggc caagcagaaa atgacctaac gctaccgcca gcgccggttt
    22981 cagcccggta gccatcggta gcttcacggg ctgaacgaca aatgcgcaaa gcaggacaat
    23041 taacgccgcg agcagtgcca tcattgccgg accgaataac gctttttccg ggatgttctg
    23101 gggaatattt cgtgcagcgt aagtcatgat taacggtacc agcagcactt tggtgataaa
    23161 ggcgctggcg gaccagatca gcagttgctc tgcggcgaac aggcacgaga gagtggcgaa
    23221 aatagacacc agcaccagcg actggcaggc gtaaaatccg catgacaggc gatagctttt
    23281 gacgctaatc acaaacagcg atgtcagcat catcagtccc gccagattat ttacgatcat
    23341 agaaccggtc attcatcatc tccttactgc aaccaggtcg cggcgattac gctggaaatc
    23401 agcgacatca caatcagtac aatcaacacc aggcgcattg accctggcag cggtgcggca
    23461 tcggcgacgg cctcgctcgg tttgccaggc acaacgcgac caaaccagcg aataaaccag
    23521 gcgaaactgg cgaccgattc aatcatcagc agaatcatgg cgggcagcag gatccagtac
    23581 tccactgaca acgcaaaacc ggcagcaaac agcgggaatt tactgaagaa gccgttgaac
    23641 ggcggcacgc cggtaatcgc cagcgctgcc acgcagaaac ccacgcctgg caacggcagg
    23701 gtgtgcaata cgccacgcag acgcggcaac aagcgcgtgc cgcagctgta actcagcgca
    23761 cctgctacaa ggaaaaacag gcttttagcg aacgcgtggt tgacgatgta ggcgatgctg
    23821 ccctccagcg ccagccgcga gccgaagatg gagagcgaca aaccgaagaa catccagcca
    23881 agttgagtga tggtcgacca ggccagcaac cgcttcatat cctgctgtgg caaatacatc
    23941 agaaagccat aaagaatggt gaccagcgcc atgaccatgc caacgccgcc aatcacatgc
    24001 gggatattgc cgccgtcgat aatggcgcgg gcaaaaatgt aaacgcccac tttcaccttc
    24061 gatgcggcgt ggagataggc actgatcggt gttggcgctt ccattgcatc cggtagccac
    24121 gcttgcatcg gtagctgggc cgatttcccc cacgcggcaa acaggatgcc gccataaacc
    24181 agataacgtg cgtcgccgtg taactcgctc atcgcgctaa gcgcaaacgt tccggtctgc
    24241 aaaaacagcg tggcggcggc aagatacaac cccaacgagc cgatatgagt gataagtaac
    24301 gcttttagtg ctgaacgctg tgctttatcg ctctggtaat aactgatcaa cgcccaggag
    24361 cagccgcccg taatttcaaa aaacaacaac tgaccgagca gcgtcgagga gagtaccagt
    24421 cccgccatcg cgccgataaa caacagcaaa aatgcgtaat aacgattcgt gccgttatgc
    24481 gggtgttcgc gatttttatc cgtcagataa cccgtggagt agatcgtgac cagcaaaccg
    24541 agaaacacca ccacaaacag aatcagcgta ctcacgcgat caatgaccag gccaaacaat
    24601 gccacatccc ccacgttgac caatgtcaac gtgacggcaa ctttatcggc ctgataaaac
    24661 gcggagatca gtgacaacat gcacagcgtg gtcagcgcgg cgaacaaaac cccccattcg
    24721 gcggcccgac gttgtggcga aaacgaaacg accagtgcgc cgataaaagg cagcaataac
    24781 gtcgtcagag caagattttc catattccgt cagtgctcct tacagaccgg ttaaccagaa
    24841 gacccatgca agcgcagcaa ggctgaagcc aagccaggtc acatggtgaa tgagtaaaaa
    24901 acgcccgcgt gccagcgtgt tttctgcgat tgaggccagt acaaaaatca gcaaaacctt
    24961 gagcagcgta acgacaagtg aagtcagcag gcaggcgaga gaaagttctt gcgcgcgccc
    25021 aaaggacaga aacagggcca caaacagtga tgccatcacg acctgtttca gccccagccc
    25081 ccatttcgct agcgccagcc cggcaccgga atattcggtc agcgggcctt cctgtaattc
    25141 ctgttctgct tcagcaacat caaagggaat ttttcccatc tcaatgaagc aggcaaaacc
    25201 acaggccagt aacgccagta cggtggttag cggcgagttc cagcccatcg ccagcgtatt
    25261 gctgatcatc tcgatatgcg tggaacctgc tatcagcgcc aataccagca gtgagagaat
    25321 aagcattggt tcgaccagaa tgccgagcgt caactcgcga ctggcaccga ctcccgcaaa
    25381 cggacttccg gtatccagcc cggaaagagc aaagaaaaaa cgaaacaggg caagaagata
    25441 gataagggtg atcagatcgc cgccgcccgc aaaaggggaa acggtaataa acagtggtaa
    25501 ggccatcgcc agcaccagca tgctgctgat taatacccac ggcatcaggc ggaacatcag
    25561 acctgaagat gtcggcgcaa cttcctggcg tttaaacagt ttgtggatat cgcgataatc
    25621 ctgccagatc cccggcccgc ggcgggagtg catacgcgcg cgtatctgcc gggaaatacc
    25681 cgtaaaaagt ggggttagca tcagcagaat cacggcctgt gctaacgcaa aaataaccag
    25741 atatccgtcg caaagagttt gtctcatggt gatttcctta gacggcaata gcgattagca
    25801 gcacaaccag tgcggcgacc acatacaggc aatagagacg aaagtcgccg ctttgcagat
    25861 gctgtatttc tttggccagc cgttgggtgg cgctcacgat ggggcggatc acccgctcat
    25921 cccagaaggg ttctgtgctc tgagccctgg cggtgacgtg cgcaaaacct ttatttagcc
    25981 tcagcgtagg gtcgagctgt tgtcgtagac gaaataacgc agagaagacc acacgcagcg
    26041 gctgcatcac gccattgcct gacggggcca tcgcattttc ccagccgtag ccgcatgccc
    26101 aggcatctcc tgtgcgacga aacgctgcgc ggcgcgaacg acaccacagc cagtaaaggc
    26161 caggcagcaa aggcatcgcc agtagtaaca gcaacagtaa tgagggggtg acctgtgtat
    26221 gaaacgtgcc gggtacaagt gctattccgc tggcgacagt gacgggaggg gtattggtaa
    26281 acgcatgggc aatatgcatt atcttcggtg ccagccaact ggcactaagc gcaattaata
    26341 cgcagagtgc cgcgagcagt agcatggcga cgatcatcgt atttggcact tcctgtgcct
    26401 cttcagcgtg tgtactgcgc ggcgcaccac agaaagtaat accgtacatt tttacgaagc
    26461 acattactgc cagcccacca gtgactgcca gcattaccat agcaatagga cccgcaagtt
    26521 gtagcgctac ggcttccaca cgacttagtg agaacagcga ctgccaggtg taccattcgc
    26581 tgataaaacc attcagcgga ggaatggctg atatcgcgag gcaaccaatc aggcatgctg
    26641 cggctgtcca cggcatccgt ttcgccagtg cccccatttt ttccatgtcg tgggtatgca
    26701 aacgcgaaat aatcgcgccc gcgccgagaa atagcagccc tttgaacagc gcatggttga
    26761 gcagatgaaa cagtgcgccg agcagtccaa ccacggtgag cagcgggtcg tgcagtgaca
    26821 gaccgaccat cgccacaccg actgccagca aaataatgcc gacgttttcg acggtactcc
    26881 aggccagcag ccgtttgata tcctgttccg ccagcgcata cagcacgccc aggagcgcgg
    26941 agattgcgcc gatcgccatc accagaatgc cccaccacag cggcaaaccc gtttgcgcca
    27001 gcaggtccat cgctactttc aggatgccga aaataccgat tttgaccatt acgccagaca
    27061 tcaacgccga agcgtgcgat ggtgcggcag ggtgagcgcg cggcaaccag ctgtgcaacg
    27121 gcatcatccc ggctttcgcg ccaaaaccga aaaaggccag caggaacacc gccgacgcca
    27181 gccccggaga aagtgaaagc gtgcggaaac tggcaaaatc gaggctgccg ctttcgcgcc
    27241 ccatcagcaa gaaggcgatc ataatcagca ccgatccggc gtgggcgata aaaaagtaga
    27301 gcatgccagc gttgatcgac gttttatcct gcctggcaat gaccagaaac caggaagaca
    27361 gcgacatcat ttcaaacagc acgatgaacc aaaaagcgtt gtccatcacc agcagggcaa
    27421 ccatcgatgc gatgaaaaga ttcataaaga agcccatcgc cgccgcgcct ttgccctcgt
    27481 attcgcgcat ataagtcaat gaatagagcg aacaaaccac caccagcaag gagataacca
    27541 gcaccataaa tgccgagagg ctatccattc gcagggtaaa atcggcaaag tcaaaagggg
    27601 tggcaaaacg ggcgactaaa gttacaccag catgtaattg cgtaattccg ctgatgatgc
    27661 caatcacgcc gcccaccagc gatgtgatgc cggaaagctt aatagccagt ctgtccagac
    27721 cgagtaaaaa cagcgaagcc agactggcaa acagatagag gatcagcgac caggttaata
    27781 attgcagggc atccattagc gttgctcctg agtgagggaa aggggatcct ccccgccatt
    27841 ggcaaaccag cttgcggcaa ggcgctgttt ttctttcatc tgtcgttgca ggctatcgtc
    27901 ggtgatcagc cgtaacgcct gattcgggca agcgcgaacg caggccggac cttctggcaa
    27961 gaaatcacac aggtcgcatt tcaccgcgac ggtctggaca cccggttccc agcgcagcaa
    28021 tgcatgttgg ggcggcacgt tttcttcgcc gtctttgagt gagccttcag cctgaaaaac
    28081 atattgcgca ggggcattca ccggacggct tcctgaagcg ctgattgcgc caaatgggca
    28141 gaccacggcg caaagcttgc agccaataca gaggctttcg ttgagttgga tcgcatcatc
    28201 cctctgggag atggcattaa ccgggcagac ctgcaggcaa ggggcttcct cacagtgatg
    28261 acacacgaca ggggcagtga gtgttgacgt cttcgccagg gccaggcgcg ggtgttgctg
    28321 taaaccttgc gttttatgca cgtccgaaca ggcagcgaga caggtattac atcctgtaca
    28381 ccacagtggt tcggccacca caaagcggtt catgtcggct ccttgaaaat gatatgaaac
    28441 attgcatttc atattgcata aaggatgcca ttctgattaa ctcaatatat tcaataagtt
    28501 atgtattgac tggattctga acggtacaaa aatgatggtc attaacgttc agcaaaaaat
    28561 ggattcgaca cccgtgtcgc aggggaaagc gagaatgtga tcttgatcac gagaagtgag
    28621 gtatgctggc cgcaagcgca gccagcacgg aatggagcaa agtaatcagg cgtgttcttt
    28681 cagccagttc agcacaacgt cgtggtgatt gctggttttg aaatcgtcaa agacatgttc
    28741 gattttgcca tcagcgtcaa tcaggaagct gatgcgatga atgccatcgt aggttttgcc
    28801 catgaaggac ttttcacccc agacgccgaa ttgttcgcac acctggtggt cctcatcaga
    28861 caggagcgta aagttaagca gctctttttc cgcaaaacgg gagagttttt cgggtttatc
    28921 ggtgctgata cccagcacat caacgcccgc ttttttcaac tcatccatgt tatcgcgtaa
    28981 gccgcaggcc tgtacggtac agccgggggt catggctttc gggtagaaat aaaccagaac
    29041 acgctgtccc tggaagtcgg tcaaattaac ttgttctccg tcttgatccg gcaagctaaa
    29101 tttcggtgcg atatcaccgg ctttcagtgg attcattact taactccatc ctgttcatca
    29161 tgttgggaat aattgacgac gttaatactg ccttgtgcat tgagttctgt acatagggct
    29221 ttgaacgctt gctcaatatt tgctgcgtcc gcagatgcgg ggctgtgggc ggttatctga
    29281 atatgcaact gcgcagcccg ttcattttca gcaggttgcg tgcgcgacac cagctccgca
    29341 atgttcatat gatgcgcgtc gaaaagtgct gtgaagcgtt caattaaatg cggggagtct
    29401 gccacatcga cctgaaccca gacagatgct ggcattggcg gacgcggacg cgccgtcgtg
    29461 cgcttcatca cgattaaaag atccagttcg gcacctttca acggtaacgt tgattcaatc
    29521 agagtgatgg cattccatga accggaaagc agcatgataa acgtgaactc ttctcccagc
    29581 atcgccaggc gactgtcttc aatattacag ccgcaactac tgacatgacg ggtgatggtg
    29641 ttcacaattc cagggcgatc ggcacccaac gcagtgatca ccagataatg ttgcgatgac
    29701 agtgtcaaac tggttattcc tttaaggggt gagttgttct taaggaaagc ataaaaaaaa
    29761 catgcataca acaatcagaa cggttctgtc tgcttgcttt taatgccata ccaaacgtac
    29821 cattgagaca cttgtttgca cagaggatgg cccatgttca cgggaagtat tgtcgcgatt
    29881 gttactccga tggatgaaaa aggtaatgtc tgtcgggcta gcttgaaaaa actgattgat
    29941 tatcatgtcg ccagcggtac ttcggcgatc gtttctgttg gcaccactgg cgagtccgct
    30001 accttaaatc atgacgaaca tgctgatgtg gtgatgatga cgctggatct ggctgatggg
    30061 cgcattccgg taattgccgg gaccggcgct aacgctactg cggaagccat tagcctgacg
    30121 cagcgcttca atgacagtgg tatcgtcggc tgcctgacgg taacccctta ctacaatcgt
    30181 ccgtcgcaag aaggtttgta tcagcatttc aaagccatcg ctgagcatac tgacctgccg
    30241 caaattctgt ataatgtgcc gtcccgtact ggctgcgatc tgctcccgga aacggtgggc
    30301 cgtctggcga aagtaaaaaa tattatcgga atcaaagagg caacagggaa cttaacgcgt
    30361 gtaaaccaga tcaaagagct ggtttcagat gattttgttc tgctgagcgg cgatgatgcg
    30421 agcgcgctgg acttcatgca attgggcggt catggggtta tttccgttac ggctaacgtc
    30481 gcagcgcgtg atatggccca gatgtgcaaa ctggcagcag aagggcattt tgccgaggca
    30541 cgcgttatta atcagcgtct gatgccatta cacaacaaac tatttgtcga acccaatcca
    30601 atcccggtga aatgggcatg taaggaactg ggtcttgtgg cgaccgatac gctgcgcctg
    30661 ccaatgacac caatcaccga cagtggtcgt gagacggtca gagcggcgct taagcatgcc
    30721 ggtttgctgt aaagtttagg gagatttgat ggcttactct gttcaaaagt cgcgcctggc
    30781 aaaggttgcg ggtgtttcgc ttgttttatt actcgctgcc tgtagttctg actcacgcta
    30841 taagcgtcag gtcagtggtg atgaagccta cctggaagcg gcaccgcttg cggagcttca
    30901 tgccccggct ggaatgattt tgccggtgac ctccggtgat tatgcaatcc cggtgaccaa
    30961 cggtagtggt gctgtcggta aggcgctgga cattcgtcca ccagcccagc cgctggcact
    31021 ggtttctggc gcgcgtaccc agttcacggg cgataccgct tcattgctgg tggaaaatgg
    31081 tcgtggcaat actctgtggc cgcaggtggt tagcgtgctg caggcgaaaa actacaccat
    31141 cacccaacgt gatgatgctg gtcagacact gaccaccgat tgggtacaat ggaaccgtct
    31201 ggacgaagac gagcagtatc gtggtcgtta tcaaatctct gttaagccgc agggttatca
    31261 gcaggcggtt acggttaaac tgctgaacct ggaacaggcg ggcaaaccgg ttgcagacgc
    31321 ggcttccatg cagcgttaca gcacggagat gatgaacgtt atttccgccg gtctggataa
    31381 atctgccact gacgccgcga acgctgcgca aaatcgtgcc tccaccacta tggacgtaca
    31441 aagtgcagct gatgacaccg gtttaccaat gctggtcgta cgcgggccgt tcaatgtggt
    31501 ttggcaacgt ctgccagcgg cgctggaaaa agtgggcatg aaagtgaccg acagcacccg
    31561 ttcgcagggc aacatggccg taacttataa gccgctgtct gacagcgact ggcaggaact
    31621 gggcgcgagc gatccaggcc tggcatccgg tgactataaa ctgcaggttg gcgatttaga
    31681 taaccgcagc agcctacagt tcatcgatcc gaaaggtcat actctgactc agagtcagaa
    31741 cgacgcgctg gtagctgtct tccaggctgc gtttagcaag taaaaataca gggctggaat
    31801 catccggccc ttttttctga tatgatacgc aaacgtgtgc gtcggcagga aaacgcgatt
    31861 ttagcggtaa ttcgcacgaa atttgtttgt cggacgtagt tcggataagg cgttcacgcc
    31921 gcatccgaca aaacatccgg cacaccagac agcaaaagat tttaaaacgt taattcacac
    31981 ccaggagtga taaagatgca aaagcaagct gagttgtatc gtggtaaagc gaaaaccgta
    32041 tacagcacgg aaaacccgga cctgttggtg ctcgaattcc gcaatgatac gtcagcaggg
    32101 gatggcgcgc gcattgagca gtttgatcgc aaaggtatgg tgaacaacaa gttcaattac
    32161 ttcattatga gcaaactggc tgaagcgggt atcccgactc aaatggagcg tctgctctcc
    32221 gataccgaat gtctggtgaa aaagctggat atggtgccgg ttgagtgtgt cgtgcgtaac
    32281 cgtgctgctg gctctctggt gaaacgtctt ggaatcgaag aaggtattga gctgaacccg
    32341 ccgctgttcg atctgttcct gaaaaacgac gccatgcacg atccgatggt caacgaatct
    32401 tactgcgaaa cctttggctg ggtgagcaaa gagaacctgg cgcgtatgaa agagctgacc
    32461 tacaaagcga acgacgtgct gaaaaaactg ttcgatgatg ctggtctgat tctggtcgac
    32521 ttcaagctgg agtttggcct gtacaaaggt gaagtggtac tgggcgatga gttctccccg
    32581 gacggcagcc gcctgtggga caaagaaaca ctggagaaaa tggacaaaga ccgtttccgc
    32641 cagagcctcg gtggcctgat cgaagcctat gaagccgtcg cccgccgcct gggtgtacag
    32701 ctggactgat ttttctggtt gcttgtttct ttagccggag ggtactccgg ctaaatgtct
    32761 ttgatacccc cgctacaata cttctcatta tctccctttg acaggaaagt cataccaggc
    32821 caatatgtat atacataacg tatatcccaa aaaggggggg ctatggaaac tactgtattt
    32881 ttaagtaatc gcagtcaggc cgtcagatta ccgaaagcgg tcgctctgcc agaagacgtt
    32941 aagaaagtag aaattattgc gattggtcgt acaagaatca tcactcccgc cggtgagtcc
    33001 tgggacagtt ggtttgatgg cgaaaatgtg agtgcggatt ttatggatat ccgtgatcaa
    33061 cccgcaatgc aggaaaggga atcattttga tgctgaaatt catgctggat accaatatct
    33121 gtattttcac tattaagaat aaaccagtac atgtcagaga gcggtttaac ctgaattctg
    33181 gccggatgtg tataagttct gtaacattga tggagcttat ctatggggca gaaaaaagtc
    33241 agatgccaga acgcaatttg gcggttattg aaggtttcgt atctcgtttg gtagtgcttg
    33301 attatgatag tgctgctgcg acgcataccg ggcaaatcag ggccgagctt gcccggcagg
    33361 gaaggcctgt tggacctttt gaccagatga tagcgggaca tgctcgcagt cgcggtttga
    33421 ttgtggttac caacaatacc cgagagtttg agcgagtggc gggaatcaga ctagaagact
    33481 ggagctaaca ttctctttta cttctaacat tcgtaaatta tataacgtgg catgaagcct
    33541 taattcctcc gccatttcat cccgctttcc tgtggtaatc ttcctctgaa ccacctacga
    33601 tcatttctat aatgaatata ttgttgaggt atctatgcgt tggcaagggc gacgtgaaag
    33661 tgacaatgtt gaagacaggc gcaacagctc cggtggtccg tctatgggcg gtcccggttt
    33721 tcgcctgcca agcggtaaag gcgggttgat tttactgtta gtcgtgctgg ttgcaggcta
    33781 ctatggtgtt gatttaaccg ggttaatgac tggacagccg gtttcccaac aacaatcaac
    33841 gcggtccatt agccctaatg aagacgaagc tgcaaaattc acctcggtga ttctggcaac
    33901 cacggaagac acctggggac aacagttcga gaagatgggt aagacctatc agcaaccgaa
    33961 gctggtcatg taccgtggaa tgacgcgcac cggctgcggg gctggtcagt ccattatggg
    34021 gccgttctat tgcccggcgg atggcacagt ttatatcgat ctctccttct atgatgacat
    34081 gaaagacaaa cttggcgcgg atggcgattt tgcccagggg tacgttatcg cccatgaagt
    34141 cggtcatcat gtgcagaaac tgttaggcat cgagccgaaa gttcgtcaac tgcaacaaaa
    34201 cgcgacgcag gcggaagtga accgcttatc tgtgcgtatg gaactccagg ccgactgttt
    34261 tgccggtgtc tgggggcata gtatgcagca gcaaggcgtt ctggaaaccg gcgatctgga
    34321 agaggcgctg aacgcggcgc aggccatcgg cgatgaccgt ttacaacagc aaagtcaggg
    34381 gcgagtagta ccagatagtt tcacacatgg cacttctcag caacgctaca gctggtttaa
    34441 acgtggtttc gacagcggcg atccggcaca atgcaatact tttggtaaaa gcatttaact
    34501 tccggggcag ggatggctga actgactgcg cttcacacat taacagcgca aatgaaacgt
    34561 gaagggatcc gccgcttgct ggtgttgagc ggggaagagg gttggtgttt tgatcatgcg
    34621 cttaagttgc gtgatgcctt acctggcgac tggctgtgga tttcgccgca gccagatgct
    34681 gaaaaccact gttcaccctc ggcgctacaa actttacttg ggcgcgagtt ccggcatgcg
    34741 gtattcgacg cccgccacgg ctttgatgcc gctgcctttg cagcacttag cggaacgttg
    34801 aaagcgggaa gctggctggt tttgttactc cctgtatggg aagagtggga aaaccaacct
    34861 gatgccgact cgctgcgctg gagtgattgc cctgacccta ttgcgacgcc gcattttgtc
    34921 cagcatctca aacgcgtact tacggcggat aacgacgcta tcctctggcg gcaaaaccag
    34981 ccgttctcgt tggcgcattt tactccccgt actgactggc accccgcgac cggcgcacca
    35041 caaccagaac aacagcaact cttacagcag ctactgacca tgcctccggg cgtggcagcg
    35101 gtaacggctg cgcgtgggcg cggtaagtcg gcgttggcag ggcaactcat ttctcgtatt
    35161 gcgggcagtg cgattgtcac cgcgcccgca aaagcggcaa cggatgtact ggcacaattt
    35221 gcgggcgaga agtttcgctt tattgcaccg gatgccttgt tagccagcga tgagcaagcc
    35281 gactggctgg tggtcgacga agccgcagcc atacctgcgc cgttgttgca tcaactggta
    35341 tcgcgttttc ctcgaacgtt gttaaccact acggtgcagg gctacgaagg caccggacgt
    35401 ggttttttgc tgaaattttg cgctcgcttt ccgcatttac accgttttga actgcaacag
    35461 ccgatccgct gggcacaggg atgcccgctg gaaaaaatgg ttagtgaggc actggttttt
    35521 gacgatgaaa acttcaccca tacaccacaa ggcaatatcg tcatttccgc atttgaacaa
    35581 acgttatggc gaagcgagcc agaaacgccg ttaaaggttt atcagttatt gtctggtgcg
    35641 cactaccgga cttcgccgct ggatttacgc cggatgatgg atgcaccagg gcaacatttt
    35701 ttacaggcgg ctggcgaaaa cgagattgcc ggagcgctgt ggctggtgga tgagggggga
    35761 ttatctcaac aactcagtca ggcggtatgg gcaggttttc gtcgcccgcg gggtaatctg
    35821 gtggcccagt cgctggcggc gcacggtagc aatccactgg cagcgacatt gcgtggacgg
    35881 cgggtcagcc ggatagcagt tcatccggcg cgtcagcggg aaggcacagg gcagcaactt
    35941 attgctggtg ctttgcaata tacgcgtgac ctcgactatc tttcggtgag ttttggttac
    36001 accggggagt tatggcgttt ctggcaccgc tgcggttttg tgctggtgcg gatgggtaat
    36061 catcgtgaag ccagcagcgg ttgctatacg gcgatggcgc tgttaccgat gagtaatgcg
    36121 ggtaaacaac tggctgaacg tgagcattac cgtttacgtc gcgatgcgca agctctcgca
    36181 aagtggaatg gcgaaacgct tcctgttgat ccactaaacg atgccgtcct ttctgacgac
    36241 gactggcttg aactggccgg ttttgctttc gctcatcgtc cgctattaac atcgttaggt
    36301 tgcttattgc gtctgctaca aaccagtgaa ctggcattac cggcgctgcg tgggcgttta
    36361 cagaaaaacg tcagcgacgc gcagttatgt accacactta aactttcagg ccgcaagatg
    36421 ttactggtcc gtcagcggga agaggccgcg caggcgctgt tcgcacttaa tgatgttcgc
    36481 actgagcgtc tgcgcgatcg cataacgcaa tggcaatttt ttcactgact ccttcaattt
    36541 tctcccatgg tcattgtgag tcagcggcgt aaaattaatg acatcagtta aggagttcgc
    36601 catgaaacat gaccattttg ttgttcaaag cccggataaa cctgcgcaac agttgttact
    36661 gcttttccac ggtgtcgggg ataacccggt ggcaatgggg gaaataggta gctggtttgc
    36721 accgctgttt cctgatgcgc tggtggtgag tgtcggtggc gcggagccga gcggtaatcc
    36781 ggcggggcgt cagtggtttt cagtgcaggg tattacggaa gataatcgcc aggcgcgtgt
    36841 ggatgccatc atgccgacgt ttattgaaac ggtacgctac tggcagaaac agagcggagt
    36901 aggggctaac gccacggcgc taattggttt ctcacaaggc gcgattatgg cgctggagag
    36961 cattaaagcc gaaccgggcc ttgcttcgcg cgtcattgcc tttaacggac gctatgcgag
    37021 cctgccggag accgcgtcta cagccacgac aattcacttg attcatggtg gtgaagatcc
    37081 ggttatcgat ctggcgcatg cagttgcggc gcaggaagcg ttaatcagtg ccggtggtga
    37141 cgtgacgctg gatatcgtgg aggatttagg ccatgcgatc gacaatcgca gtatgcaatt
    37201 tgccctggat cacttgcgtt ataccattcc gaaacattat ttcgacgaag cgctaagtgg
    37261 cggtaagcct ggcgatgatg atgtgattga aatgatgtaa aagacagatt acttggcctg
    37321 ataagacgcg tgagcgtcgc atcaggcatc gttcaccatt gccggatgcg gcgttaacgc
    37381 cttatccggc ctacggttga gcactcagct tacttcttcg gttgatcctt tttcggccag
    37441 tcgtcgtcat cgtcccactt atcgttgaaa tcacgatgcg ggggaagctc cggcttgttg
    37501 gcgagaaatt tcttatggtc gacgcgcttg agatctttaa tcacgttcag caggacgcct
    37561 accaaaaaga caatcaccag aatccaccaa tatttagcca gccagtccat gcttatttcc
    37621 tcttgcagaa ccactcatca ggcgacgagc tgttccatga tacgttgata catacgggca
    37681 agtagctgca ggttggcagc gttcacacat tcattaattt tatgaatagt ggcattgacc
    37741 ggcccgagtt ccaccacctg cgcccccatg cgagcaataa agcgcccgtc ggacgttccg
    37801 cctgtggtca gtagctgcgg tttaatttca ttatagtgct caaccgcgtt aacgaccgca
    37861 tccaccagtt taccgcgcgc ggtcaaaaat ggctgcccgg aaagccacca gtccaccgta
    37921 taacgcagtt gatgtttttc aagcagggca agcacctgtg ctttgatcat ctcatcagtc
    37981 agttcggtgc tgaagcggaa gttaaactgc acaaacagtt cacccggaat aacgttgtta
    38041 ctgcccgttc ccgcctgaat attggcaatc tgcatactgg tcgccgggaa gaattcattg
    38101 ccctgatccc actcaatagc cactaattca ttaaggaaag gtgctgcgcg atgtaccgga
    38161 ttgtcagcca gatgcgggta ggcaacatgc ccctgaacgc catgaatggt gaggttgcag
    38221 gttaacgatc cgcgacgacc atttttcacc acatcaccta ccacttcgat actcgacggt
    38281 tcgccgacca ggcagtaatc gagacgctca ttacgtgcca ttaacgcttc gacgactttt
    38341 accgtaccgt tgtgtgcact ggcttcttca tcagaggtga tcagaaatgc cagtcgcccc
    38401 gtatggttgg gatgttgtgc gacaaagcgt tctgccgcta ccaccatcgc cgccagcgag
    38461 cctttcatat ctgccgcacc gcgcccgaat aacatgccat cacgaatggt gggttcaaac
    38521 ggcggattga tccaacgatc ggcgtcgcca ggcggcacca cgtcggtatg cccggcaaag
    38581 gctaacgttt caccttgccc acgccatgcc caaaaattct gcgtatcggc aaagtccatg
    38641 cgttcaacgg taaaaccgat cgcctgcaaa cgttcaatca acaaagcctg gcatcctgcg
    38701 tcatcaggac tcagggaagg gcggcgaata agctgttgtg tcagctcaat aaccgggcac
    38761 gacatagact acacctcatg gaaaaattgc tgataactgg aatcactgaa acccagcagc
    38821 ataggcttac caggcgcgca gagcaatgga cgtttgataa ttgcaggcat ttcagtcatt
    38881 aatgccgccg cagaggccgc atcggtgatt ttattgcggg tggtttcgtc cagtttacgc
    38941 caggttgtac cacgggtgtt gagtaacgct tcccagccta attcgttgat aaaaccgttc
    39001 aataattcgc tgtccagccc atcgacgcgg taatcatgaa aacgatagtc gatgttattg
    39061 gcttccagcc aacggcgagc ctttttaatg gtgtcacaat ttttgatgcc gtaaagtgta
    39121 accataatga atcctttttc gatttatgag aataaatact catttaaggg ggaaaacatt
    39181 ttgcctgcaa atgattatca gaaaa
//
LOCUS       DAZGLJ010000022        38730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000022 DAZGLJ010000000
VERSION     DAZGLJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.022"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..733
                     /gene="nadE"
                     /locus_tag="ACHK49_001025"
     CDS             <1..733
                     /gene="nadE"
                     /locus_tag="ACHK49_001025"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO9261711.1"
                     /translation="YLQTYPFIKSLVLGISGGQDSTLAGKLCQMAINELRQETGNESL
                     QFIAVRLPYGVQADEQDCQDAIAFIQPDRVLTVNIKGAVLASEQALREAGIELSDFVR
                     GNEKARERMKAQYSIAGMTSGVVVGTDHAAEAITGFFTKYGDGGTDINPLYRLNKRQG
                     KQLLTALGCPEHLYKKAPTADLEDDRPSLPDEVALGVTYDNIDDYLEGKNVPEQVART
                     IENWYLKTEHKRRPPITVFDDFWKK"
     gene            963..1850
                     /gene="cho"
                     /locus_tag="ACHK49_001026"
     CDS             963..1850
                     /gene="cho"
                     /locus_tag="ACHK49_001026"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO9261712.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(1810..2385)
                     /gene="ves"
                     /locus_tag="ACHK49_001027"
     CDS             complement(1810..2385)
                     /gene="ves"
                     /locus_tag="ACHK49_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO9261713.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(2588..3073)
                     /gene="spy"
                     /locus_tag="ACHK49_001028"
     CDS             complement(2588..3073)
                     /gene="spy"
                     /locus_tag="ACHK49_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO9261714.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPIMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIVASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(3403..4371)
                     /gene="astE"
                     /locus_tag="ACHK49_001029"
     CDS             complement(3403..4371)
                     /gene="astE"
                     /locus_tag="ACHK49_001029"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO9261715.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(4364..5707)
                     /gene="astB"
                     /locus_tag="ACHK49_001030"
     CDS             complement(4364..5707)
                     /gene="astB"
                     /locus_tag="ACHK49_001030"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO9261716.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(5704..7182)
                     /gene="astD"
                     /locus_tag="ACHK49_001031"
     CDS             complement(5704..7182)
                     /gene="astD"
                     /locus_tag="ACHK49_001031"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO9261717.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(7179..8213)
                     /gene="astA"
                     /locus_tag="ACHK49_001032"
     CDS             complement(7179..8213)
                     /gene="astA"
                     /locus_tag="ACHK49_001032"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO9261718.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(8210..9430)
                     /gene="astC"
                     /locus_tag="ACHK49_001033"
     CDS             complement(8210..9430)
                     /gene="astC"
                     /locus_tag="ACHK49_001033"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO9261719.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQLGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            9876..10682
                     /gene="xthA"
                     /locus_tag="ACHK49_001034"
     CDS             9876..10682
                     /gene="xthA"
                     /locus_tag="ACHK49_001034"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO9261720.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            10849..11559
                     /locus_tag="ACHK49_001035"
     CDS             10849..11559
                     /locus_tag="ACHK49_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO9261721.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLLGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            11564..12241
                     /gene="ydjY"
                     /locus_tag="ACHK49_001036"
     CDS             11564..12241
                     /gene="ydjY"
                     /locus_tag="ACHK49_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO9261722.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            12256..12963
                     /locus_tag="ACHK49_001037"
     CDS             12256..12963
                     /locus_tag="ACHK49_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO9261723.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            12963..13511
                     /locus_tag="ACHK49_001038"
     CDS             12963..13511
                     /locus_tag="ACHK49_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO9261724.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            13521..14687
                     /locus_tag="ACHK49_001039"
     CDS             13521..14687
                     /locus_tag="ACHK49_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO9261725.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            14660..16195
                     /locus_tag="ACHK49_001040"
     CDS             14660..16195
                     /locus_tag="ACHK49_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO9261726.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPTLLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            16195..16848
                     /locus_tag="ACHK49_001041"
     CDS             16195..16848
                     /locus_tag="ACHK49_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO9261727.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMEQWSENYNKLR"
     gene            16915..18222
                     /gene="ynjE"
                     /locus_tag="ACHK49_001042"
     CDS             16915..18222
                     /gene="ynjE"
                     /locus_tag="ACHK49_001042"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO9261728.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(18231..18851)
                     /locus_tag="ACHK49_001043"
     CDS             complement(18231..18851)
                     /locus_tag="ACHK49_001043"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO9261729.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            18938..19345
                     /gene="nudG"
                     /locus_tag="ACHK49_001044"
     CDS             18938..19345
                     /gene="nudG"
                     /locus_tag="ACHK49_001044"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO9261730.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(19311..19583)
                     /locus_tag="ACHK49_001045"
     CDS             complement(19311..19583)
                     /locus_tag="ACHK49_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO9261731.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            19819..21162
                     /gene="gdhA"
                     /locus_tag="ACHK49_001046"
     CDS             19819..21162
                     /gene="gdhA"
                     /locus_tag="ACHK49_001046"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO9261732.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLERQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(21279..22319)
                     /locus_tag="ACHK49_001047"
     CDS             complement(21279..22319)
                     /locus_tag="ACHK49_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO9261733.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEYFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(22447..24408)
                     /gene="topB"
                     /locus_tag="ACHK49_001048"
     CDS             complement(22447..24408)
                     /gene="topB"
                     /locus_tag="ACHK49_001048"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO9261734.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQSVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(24413..25456)
                     /gene="selD"
                     /locus_tag="ACHK49_001049"
     CDS             complement(24413..25456)
                     /gene="selD"
                     /locus_tag="ACHK49_001049"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO9261735.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIH"
     gene            complement(25573..26124)
                     /locus_tag="ACHK49_001050"
     CDS             complement(25573..26124)
                     /locus_tag="ACHK49_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO9261736.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            26285..28141
                     /gene="sppA"
                     /locus_tag="ACHK49_001051"
     CDS             26285..28141
                     /gene="sppA"
                     /locus_tag="ACHK49_001051"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO9261737.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            28308..29324
                     /gene="ansA"
                     /locus_tag="ACHK49_001052"
     CDS             28308..29324
                     /gene="ansA"
                     /locus_tag="ACHK49_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO9261738.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            29335..29976
                     /gene="pncA"
                     /locus_tag="ACHK49_001053"
     CDS             29335..29976
                     /gene="pncA"
                     /locus_tag="ACHK49_001053"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO9261739.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(30137..30409)
                     /locus_tag="ACHK49_001054"
     CDS             complement(30137..30409)
                     /locus_tag="ACHK49_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO9261740.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(30451..30864)
                     /gene="msrB"
                     /locus_tag="ACHK49_001055"
     CDS             complement(30451..30864)
                     /gene="msrB"
                     /locus_tag="ACHK49_001055"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO9261741.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            31206..32201
                     /gene="gapA"
                     /locus_tag="ACHK49_001056"
     CDS             31206..32201
                     /gene="gapA"
                     /locus_tag="ACHK49_001056"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO9261742.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            32285..33169
                     /locus_tag="ACHK49_001057"
     CDS             32285..33169
                     /locus_tag="ACHK49_001057"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO9261743.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(33220..34074)
                     /gene="yeaE"
                     /locus_tag="ACHK49_001058"
     CDS             complement(33220..34074)
                     /gene="yeaE"
                     /locus_tag="ACHK49_001058"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO9261744.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(34164..34910)
                     /gene="mipA"
                     /locus_tag="ACHK49_001059"
     CDS             complement(34164..34910)
                     /gene="mipA"
                     /locus_tag="ACHK49_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO9261745.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            35346..37280
                     /gene="yeaG"
                     /locus_tag="ACHK49_001060"
     CDS             35346..37280
                     /gene="yeaG"
                     /locus_tag="ACHK49_001060"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO9261746.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            37393..38676
                     /locus_tag="ACHK49_001061"
     CDS             37393..38676
                     /locus_tag="ACHK49_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO9261747.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         9352 a        10186 c         9781 g         9411 t
ORIGIN      
        1 ctacctgcaa acttatccgt tcattaaatc actggtgctc gggatcagcg gcggtcagga
       61 ctccacgctt gccggaaagc tgtgccagat ggcgattaat gagctgcgcc aggaaactgg
      121 caacgaatca ctgcaattta ttgccgtacg cctgccctat ggtgttcagg ctgacgaaca
      181 agattgccag gatgccattg cctttattca accggatcgc gtattaaccg ttaatatcaa
      241 gggcgcggta ttggctagcg agcaggcatt gcgggaagca ggcattgaac tgagcgattt
      301 tgtccgtggc aatgaaaaag cgcgtgagcg gatgaaagca caatatagca ttgcgggtat
      361 gaccagcggt gtcgtggtgg gcaccgatca tgcagcagaa gccattaccg gattcttcac
      421 taaatatggt gacggcggta cggatattaa tccgctgtat cgtctcaaca aacgccaggg
      481 taaacagttg ctgacggcat taggttgccc ggaacacctt tataagaaag ccccaacggc
      541 cgatctggaa gatgatcgcc cttctctgcc agatgaagtg gcactcggcg tgacctatga
      601 caatatcgac gactatctgg aagggaaaaa cgtacctgaa caggtcgcca gaacaataga
      661 gaactggtat ctgaaaaccg aacataaacg ccgtccgcca attaccgttt tcgatgattt
      721 ctggaaaaag taataatttg cacataatgg tttgtgcgaa aaagagtaat ttgttcacgc
      781 cggatgcggc gtgaacgcct tattcgacct acaaaactat gcaaattcaa tatattgcag
      841 gagtcgaata ggcctgacag gcgtagcacg tcagacggtg taacctttgt catcgacccg
      901 cctctttttt aatcgcttcc cgcctgttac actggataga taaccagcat tcggagtcaa
      961 cagtggtacg gcgtttaact tctccgcggc tcgaatttga agctgcggca atttatgaat
     1021 atcccgaaca tttacgttca ttccttaatg acttacccac ccgaccaggg gtgtatctgt
     1081 ttcatggcga aagtgacacc atgccgctct atatcggcaa aagcgttaat atccgcagtc
     1141 gggtgctttc tcatttacgc accccggatg aagccgccat gctacggcaa tcccggcgaa
     1201 ttagctggat ctgcaccgca ggcgaaatcg gcgctctgct ccttgaagcg cgattaatca
     1261 aagaacaaca gccgctgttt aataaacggt tgcgccgcaa tcgccagctc tgtgccctgc
     1321 aattaaatga aaagcgcgtc gatgtggtgt atgccaaaga ggtggatttt tcacgagccc
     1381 ccaacctgtt tggcctgttt gccaataggc gcgcagcttt gcaagcattg cagagcatcg
     1441 ctgatgaaca aaaactttgt tatggcctgc tgggactgga accgttaagt cgcggtcgtg
     1501 catgttttcg ttcagcgcta aaacgttgcg ccggagcatg ctgcggtaaa gagagccatg
     1561 aggaacatgc gctacgcttg cgccagtctc tggagcgttt gcgggtggtg tgttggcctt
     1621 ggcaaggggc ggtggcgctg aaagaacagc acccggaaat gactcaatat catattattc
     1681 aaaactggct gtggctgggg gcggttaatt cgctggaaga cgcgacaacg ttaattcgga
     1741 cacccgccgg gtttgatcac gacggttata aaattctttg taagccgctg ctttccggta
     1801 actatgaaat tactgaactt gatccggcga atgaccagcg agccagttga tttcactgaa
     1861 caacagttta ccttgcggtt gtaaaagccg cagggtgtgc ctgccatcga accagcacgc
     1921 gccctggtcg gtagtgagca gtttgtcgcc cagttgccac gccccgttaa tcacaaacac
     1981 taccccgccg cgtgagccaa aggtagtaaa cgtacgttcg gcaattctga ctttcgcctt
     2041 acagacatcc aggcgcgtca taatattgaa atccatcgac atctgccctg ccgtcagttt
     2101 cgcttttacc acctggtccg ccgcaaaggc aaaaggctgt aacggtttta aggtatggtt
     2161 aaagtggtct gcgctttcaa ggaacatctc gccgccttcc agcaacgtca ctatcctttc
     2221 cataccggga aacaaagaaa actccccatt cgccgcgatg gatgcaatgc tggcacgcca
     2281 gtaaaaatca cgttttgccg gtgggaaagt gcaaatttcg cgcgtttcac cggcagcatt
     2341 tcgccacaga ttcaccgaca ttttacgcat atcaaagtat tccatactcg ctcccggccc
     2401 gttcttttca ttgttatcgt tcaggtaacc gtggatggtt atcggcaact cgggtgattt
     2461 acgttagtgg tgatcaggct ttttttacaa ctgtttgaga agagaaaaga aaaccgccga
     2521 tcctgtccac cgcattactg caaggtagtg gacaagaccg gcggtcttaa gttttttggc
     2581 tgaaagatta ttcagcagtt gcaggcattt taccttttgc cgctggacgt tctgtcagac
     2641 gcttctcaaa attagcatta aattgctttt tctgttccgg cgtcaggatg ttgtaaattt
     2701 tgttctgggt ttccatgtgc gccagcatgt tagctttgcg ctgttcttcc atttttgcga
     2761 tctgcgcttc agcttttgct ttatcgaagg tatcgctggc aacgatgtca tgcattgcgc
     2821 ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg gcctttcatg atttcgcgga
     2881 tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa catcatgtcc tgatgcggac
     2941 cgaacttgcc tttgtggtgc ataatcggct tcgcgtcagc cggtgctgcg gtagtggtgt
     3001 ctgcggcatg ggccaggtta gccgcgccaa gagccagggt agaggcaaca aacagtgcag
     3061 ttaatttacg catattctat atccttcctt tcagttattt attacggctt tcttaagtag
     3121 cgtgccgtgt tgacgagatt aactttactg actttagcgt caattaatca gagcaacggt
     3181 aaaacaatga aagtgtaaaa aacacttttg cgccaattat ggagaaaaaa agaaaatttg
     3241 atgaagagtg atgagagaat attacaacac gatgattttg cagagattat gaagaactat
     3301 accggatgac tggtgataaa taaagcaaat aaccaggatt aatctgtatt aatttataag
     3361 aaagcaactt aatacccgca gagtgatttc tgcgggtaag tattagctta ttttttcgag
     3421 cattaatccc gcgcgtaatc ccaacgctac caacggatta gggaataaca catactctac
     3481 atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc agcaatgttc ctttctcaaa
     3541 cggcataaaa ttcagcgtgt cacttgccat atgcatttcg aaggacggcg agtggcgagt
     3601 aatttgcgaa accacccgat aacggagcgg cggtgttctc acgataccga cactctcacc
     3661 agatagcagc gcagcaattg cgctggcagt tactgcaaac tggcgaagat cgttttgccc
     3721 aaagggcaac gctttgccaa gttccagcgt acaggccagc gcgccaaaat gtctggcgct
     3781 gaaatgggta aacgtaccac caggttcctg atggaacacc agcgcctcca gccccgccgc
     3841 acccagccac gtcagaaatt tctcgtccca gggaatgtcg cgttgcggta atacaccgaa
     3901 ctgcggatgc aaggagccac gaattgcggt atgtagatca aggtgccagc gcacagattc
     3961 tttgccctgg tcataaaaat cttccaggca ctgttccagt tcgcgcgccc gacaggtttc
     4021 tccgctttca gcaaatagct gccaacgacc gccaaacatt cgattcatat cgctatggca
     4081 ataacgtttc ccttgcttca gcgcaggagg attcccgagg atcaccagca accgccaacg
     4141 taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg tccagcatct ccacaggtgc
     4201 cgtctcatta ccgtgtattc ccgctgaaat caccagtgcg ccttgcggtg gcgttaatgg
     4261 cgtcagttcc agcacaccat cgcccagcca gcgccagcga acgccgttga tttctcgctc
     4321 ggtgataacc ggttttttac ccgttaaggt cagagcaaga aaattatcca ttgccccctc
     4381 cctcgcgctg gaacggataa accgaaccga gattcagtaa ttggctcaat acatccagtg
     4441 cttcccgccc ttcgcgcagc aattgcgggt cggccagatc ggcagcagta aggcgatcgc
     4501 ggtagtaacg atccacccag tcattgagcg cattaaacag cgtatcgttc atcatcaccg
     4561 ccggattcac cgcccggcgt tcttcttctg tcaataccac ccgcaaccgc aggcacgccg
     4621 ggccgccgcc attcgccatg ctttcacgta aatcaaagac ttttagttcg ctaatcgggt
     4681 tgtcagcggc aaggagttca ttgagataac cccatactcc ggcgtgttcc cgacactcct
     4741 gaggcagcac caacatcata gaaccatcat cgcggctcaa cagttggctg ttaaacagat
     4801 aggtagacac cgcatccgac acggaaacct gagttgccgg aacttctatc gccataaaac
     4861 cgttcacccg cgcacgcagg tttgccagta actgtgactg gcgagcgaac gcctgttggt
     4921 ggcaaaacag cacctggcgg ttactcacgg caatcacgtc attatgaaaa acgccctggt
     4981 cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg gggattcacc tgattcagcc
     5041 ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg ataacgggaa ggccgggtat
     5101 cattgccttc ttctcgcccg tagacaaaaa gttgcatacc cggctcaccg taatgaccgc
     5161 cgagacgatt gtggtttgcc gccccctcat caccgagcaa cgctacctgc ggcaacgccg
     5221 aatgaacgct aaatttctct tcgtcgttaa aaatcgcttt taaaagtgat tcggtgacgt
     5281 gcgcttccag cgagcggtga aatttattgt tcaggttggc aacggtgaga tgcactttgc
     5341 catccagcgt atcggcagat ggcgcgatcg ttgccgcatt ggctacccac attggcgaag
     5401 cggaactgac gctggaaagc cagtgcggtg cctggcgtgc aactttttcc agtacctgct
     5461 catcgctacc actgaatccc aactgacgca gcaccggaat aaacggacgc tcgtgcggcg
     5521 ggatcacggc ctgggggaat cccgcatcgg caagggcttt cattttcagt aagccctgtt
     5581 ttgccgcctg tcgcgggtta gacacctgaa aacggtgacg ggtagaggct tcattaccaa
     5641 acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc gtcgaaatta acttcccagg
     5701 cgttcatcgc accacctcat cggaaaaatc cagcccgggg ttaagcgttg cgggcaaagt
     5761 taacgagtcc gactccaggc tcgccatagg ccatgcgcag taatctgcgg cataccaggc
     5821 gctggggcga tggttaccgg atgcaccaat gccgccgaat ggcgcggtac tggcagcacc
     5881 agtaagcggt ttgttccagt taacaatccc cgcccgcgcc tccagcaaca gttgatcgaa
     5941 tttttcccgc tcgggggaaa ccagaccgca agagagtccg aagcgagtgt tattcgccat
     6001 tagaatcgct tcctcgaaag aatcataacg ccagacgcgc aataacggtc cgaacacctc
     6061 ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc cccggcgtca gcaacgatgt
     6121 ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg cccatcgctt ccagttgctg
     6181 ccaggcagta accacctgct gtgcggcctg ttcagaaatc agcccgccaa taaacggctg
     6241 cggttcgtca tcccagttgc ccggcgttaa tcgctggctg acggcaacca gacgagcaag
     6301 aaacgcatcg ccctgcgctc cgcttttgag caataaacgg cgggcgcagg tgcagcgttg
     6361 cccggctgtg acaaacgccg actgaatggt cagatggaca gccgcgtcga tatccgccac
     6421 ctcatcgata attagcggat tattaccgcc catctcaagg gcgagaattt tctccggctg
     6481 accggagagc tggcgatgca gctggtagcc ggtattggcg ctaccggtaa acagcaaacc
     6541 gtcgagatcc tccagcgcac tcagcgcctg acccgtttca cgcccgccct gcaccaggtt
     6601 cagtacgccc ggcggcaagc cagcctgctg ccataaacgc attaccgctt cgccactcca
     6661 cggtgtcagt tcgctgggtt taaagataat ggtgttacct gccagcaatg ccggaacgat
     6721 atgtccgttc ggcaaatgac cagggaaatt atacggccca aacaccgcca gcacgccgtg
     6781 cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca ctacgctgct cgccggtacg
     6841 aacgtgatac gccttaattg atatcgcgat tttattgatc atcgccgtca cttcggttgc
     6901 cgcttcccag cgcggcttac ccgtttctct ggcaataatc gcggttaatt cgcctttatt
     6961 cctttccagc aatccggcaa agcgttcgac aacgacctga cgttcagcca atgagagccg
     7021 cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc tgttcgacct gagcggcatc
     7081 ggcatcattg ccttgccata acacctcgcc cgataccgga ttacgcttca cacgcgatgc
     7141 gccctggccc gttatccagt caccgttaat ccataaagtc atgctgtttt ctcctctgcg
     7201 cacaggcgca ccagacgaac gcgatcccct gcgtggcatt tgagggcatc cagttgtgcg
     7261 gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg ccagcaccac gcggaaatgg
     7321 tgataatttt cattggcgac caggcaggcc ggaaaatcgc cctgcgcagg ctgcccttct
     7381 gccacttcca ccagccgact tttacgaatg gcgcgcacgc ggtcgatgtc acactcaagc
     7441 gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac gaaaaccttc tttctccagc
     7501 accgcgcggg caggcgcggt ttgcggatgt acctgaccga tgacgtcctg ggcttcctgg
     7561 gataaaaagt gggtatagat cggatgtttc ggcatcagtt ctgcaataaa tgccttttgc
     7621 ccggtgccgc agagaaaatc ggcgcggcta aaatccatcg aaaagaagcg tttaccgagg
     7681 ctttgccaga acggtgaata gccgtgttcg tcaatcaccc cgcgcatttc agcaaccact
     7741 ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa agcgcgattt cgacagcaaa
     7801 tagccgttgc cctctttgcg ccagtccggg tcgagaaaca gcgtgcacag ctcgctgctg
     7861 ccggtgtgat cgttactgag aaacagcgtc ggcaatgcgt tatagacatt cagctctttt
     7921 gaggcgtgaa ccaacgtgcc gacgcgatag ttgtaccagg gatcgttcag cccaaccgcc
     7981 acctcaatgg cacaaatccc cgccacggtg cctgtctcgc tatcttccag cacgaacaca
     8041 tagccctgct cacttttggg cagttcgcct tgccaggttt tgattgccct ttcgatacgc
     8101 gccgaaagcg tggcttcatt ggcgggaagc gacgtcaggc cgccgcccgt tttgctggca
     8161 agctgcatca gcgccgagac atctgatcgc tcaacgggac ggatgaccat catgatgaac
     8221 ctcggctaac aaagtgttcg caagcagctg caaagcgatc cagtccggtc gtcacctctt
     8281 cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt gccacccgca atcagtacca
     8341 tcacgcctgc tttcgccgct tcctgagaga tctgtttcgc ttgcccggcg taatcggcat
     8401 tcagtacaca gccaatcagc aaacctaagc cgcgaacttc actgaacaaa ccatagcggt
     8461 gattaatagt attaagacgc tcaacaaacc agtcgtgacg ctgtttaacg ccattaagca
     8521 tctctggtgt gttgatgagc tccagcactt tgcctgccac cgccgaggcc agcggattac
     8581 cgccataggt ggtgccatga gtgccaacgg tcatcacgcg ggcgcactct tcggttgcca
     8641 acaacgcacc gaccgggaaa ccgccgccca gcgctttggc ggtagttaac agatcaggcg
     8701 tcacgccgta gtgcatatag gcatacagtt ccccggtgcg cccgacgccg gtttgtactt
     8761 catcaaaaat caacagcgca ttgtggcggt tacacaattc acgcagacct tgtaaaaacg
     8821 cgttgctggc tggcaccaca ccgccttccc cctggatggg ttcgacaatc accgcacagg
     8881 tagagtcgtc aatcagcgcg ctggcagagt taatatcgtt atatgcagca tgacgaatat
     8941 ccgccggcag tggcgcaaaa tcctgtgaat aggctggctg cccacccgca ctgacagtaa
     9001 acagcgtgcg accatgaaac gcatttttga acgccacgat gccgctctta tggctgccgt
     9061 agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc ttcgttggct tccgcaccgg
     9121 agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat caattttttc gccagtcgca
     9181 gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa cttactcgcc tgttcgttca
     9241 gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt caccgcaatg ccacccgcga
     9301 agtcgatata ctctttcccc agctgatccc acaagcgcga accttcgcca cgtaccggta
     9361 taaagggtgc cggagcgtaa acaggtatca tccattcatc aaagttttca cgcgtaattg
     9421 gctgagacat agcgacctct acagtaaata attcgttatt tatatgttaa caataagtaa
     9481 tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc cagtgataaa agtgcataaa
     9541 cggcggaggc taactggaaa tcaaggagtt ataaccaaac catatgcatt taaagtgcat
     9601 ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa taaaaaacgc aatgttatgc
     9661 agaagtaaaa tataattctg gaattgtgat cattgacgaa atttactgga aattactgcg
     9721 ccattctgac gcagcgcgca ccaaaagcgg gcattttttg cgccatcgtt gacatcatta
     9781 acaaccatcg atcaaatcac ttaacaacag gcggtaagca acgcgaaatt ctgctaccat
     9841 ccacgcactc tttatctgaa taaatggcag cgactatgaa atttgtctct tttaatatca
     9901 acggcctgcg cgccagacct caccagcttg aagccatcgt cgaaaagcac caaccggatg
     9961 tgattggcct gcaggagaca aaagttcatg acgatatgtt tccgctcgaa gaggtggcga
    10021 agctcggcta caacgtgttt tatcacgggc agaaaggcca ttatggcgtg gcgctgctga
    10081 ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg tgacgacgaa gaggcgcagc
    10141 ggcggattat tatggcggaa atcccctcac tgctgggtaa tgtcaccgtg atcaacggtt
    10201 acttcccgca gggtgaaagc cgcgaccatc cgataaaatt cccggcaaaa gcgcagtttt
    10261 atcagaatct gcaaaactac ctggaaaccg aactcaaacg tgataatccg gtactgatta
    10321 tgggcgatat gaatatcagc cctacagatc tggatatcgg cattggcgaa gaaaaccgta
    10381 agcgctggct gcgtaccggt aaatgctctt tcctgccgga agagcgcgaa tggatggaca
    10441 ggctgatgag ctgggggttg gtcgatacct tccgccatgc taatccgcaa acagcagatc
    10501 gtttctcatg gtttgattac cgctcaaaag gttttgacga taaccgtggt ctgcgcatcg
    10561 acctgctgct cgccagccaa ccgctggcag aatgttgcgt agaaaccggc atcgactatg
    10621 aaattcgcag catggaaaaa ccgtccgatc acgcccccgt ctgggcgacc ttccgccgct
    10681 aatttagcag ctctcctggc tcaaactggg tcaggagaat taaccttgag aaaaatcaac
    10741 aaactgtcag taatgatttg ttgcctgccg tcctttgtta taccgtcact gcgtttttag
    10801 ttgtctgacc acttctctat tatcaagttt gatataggaa actccacgat gaacgctgag
    10861 cgtaactttc tgtttgcctg tcttattttt gcgctggtca tttacgctat ccacgctttc
    10921 ggtttattcg atctgctcac cgatttaccc cacttacaga cactcattcg ccagagcgga
    10981 ttgttcggtt atagcctcta tattctgtta ttcatcattg ccaccctctt tctgttacca
    11041 ggaagcatac tggtgatcgc tggaggaata gtttttggcc cgctcttagg gacactactc
    11101 tcattaattg ccgcaacgct ggcctcctcg tgctcattcc tgctggcgcg ctggctggga
    11161 cgtgatttac tgctgaaata cgtgggccat agccatacct tccaggccat tgaaaaaggc
    11221 attgcgcgta acggtattga ttttcttatt ctgacccgct taatcccgtt gtttccttac
    11281 aatattcaaa attacgctta cggattaacc accatcgcct tctggcctta tacccttatt
    11341 tcggcactca cgaccctgct cggtattgtt atttataccg tgatggcaag cgatctcgcc
    11401 aatgaaggca ttacgctgcg ctttatttta caactctgtc tggcgggcct ggcgctgttt
    11461 attctcgtcc agctcgcaaa actctacgcc cgacacaaac atgtggatct gtctgcttcg
    11521 cgccgcagcc cacttactca cccaaaaaat gaaggataga acgatgtcgc aacattattc
    11581 agtgtcatgg aaaaaaggac tggctgcact ctgcttactg gctgttgccg ggcttagcgg
    11641 ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac gacggacttt cgaacagcca
    11701 accgctgcgc gtcgatgcca ataaccatac ggtaaccatg ctggtgcaaa ttaatggtcg
    11761 tttcctcacc gacgacactc gtcacggtat tgtgtttaaa gatggctcca acggacataa
    11821 atcgctgttt atggcttatg cgaccccgaa agcattttat gaagccctga aagaggcagg
    11881 tggtacgccg ggcgaaaaca tgacgatgga taataaagaa acgactcatg tcacaggcag
    11941 caaactggat atttcggtca actggcaagg ggcggcaaaa gcgtattcct tcgatgaagt
    12001 gattgttgac agtaatggca agaaactgga catgcgcttt ggcggtaatt taacggcagc
    12061 agaagagaag aaaacgggtt gcctggtgtg tctggatagc tgcccggtcg gcatcgtcag
    12121 caatgcaaca tacacttatg gtgcggtgga aaaacgcggc gaagtgaaat tcaaaggcaa
    12181 tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt acctttaaaa tcaccgaata
    12241 aagccaggat aaaggatgat gaagatacaa tcgcgtaaaa tctggtacta ccgtataacc
    12301 ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc tgcttcccgg cgtccatgag
    12361 tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc aacagggtat agaacgcttt
    12421 attcagtctt acggcgcact ggcggcagtt gtctcgttct tgttgatgat tttacaggcc
    12481 attgctgcac cgctacctgc gtttttgatc acctttgcca atgcgtcgct gtttggcgcg
    12541 ttctggggcg gcttgctgtc gtggaccagt tcgatggccg gcgcggcgct gtgctttttt
    12601 atcgccagag tgatgggccg cgaagtggtg gaaaaattaa ccggcaaaac cgtgcttgac
    12661 agtatggacg gctttttcac tcgctacggc aaacacacca tcctggtctg tcggttattg
    12721 ccttttgtcc ctttcgatcc aatcagctat gctgccggtc tgacttcaat acgttttcgc
    12781 tcgtttttta tcgccaccgg gcttggtcaa ttaccggcga ctattgttta ttcctgggcg
    12841 ggcagcatgt taacaggcgg tactttctgg tttgtcaccg gactgtttat tctgtttgcc
    12901 ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg agcgccagaa gaggaatgcc
    12961 tgatgggttt accgccgctt agcaaaattc ctttcatttt acgtccgcag gcgtggctgc
    13021 atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg gtgggggcgg atcccgttta
    13081 tcttttattt ggtgtcaatg tttgttgggt ggctggagcg caaacgctca ccgctcgatc
    13141 cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat gtgcctgtgt gagttttgcg
    13201 tggatatcac cagtatgaaa gtcgccgagc gtaccggcag caccgataaa ctgctggcag
    13261 tggctgactg gcggcaaagc ccgctcttca gcgatgaaga acggctggcg ctggagtacg
    13321 ccgaagccgc aagcgtaacg ccgccaacgg tcgatgatgc cctgcgcacc cgactggctg
    13381 cgcattttga cgctcaggcg ctcaccgaac tgacggcatt gatcggcctg caaaacctgt
    13441 cggctcgttt taattctgcc atggacattc ccgctcaggg gctgtgccgt attccggaaa
    13501 aacgttctta aggagagaag atgcgccatt gtgggtggtt gctgggattg ttatcgctgt
    13561 tttctctggc aacacatgcc agtgactggc aggaaattaa aaatgaggcc aaagggcaaa
    13621 ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa ccgctatctc gactgggtta
    13681 gcggcgagat gaaaacccat tacgctataa acctgaagat tgttcgtctg gcggatgccg
    13741 cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg acgtaaaacg ggcggctcgg
    13801 tggatctgct ctgggtgaac ggcgaaaact tccgcacctt aaaagaggcc aatttattac
    13861 aaacgggctg ggcggagact ctgcccaact ggcgctatgt cgacacacag ctgccggtgc
    13921 gggaagattt ttcagtgcct acagaagggg ctgaatcgcc ctggggcggc gcacaactga
    13981 cgtttatcgc ccgccgcgat gttacgccac agccaccaca aacgccgcaa gccttactgg
    14041 agtttgctaa agccaatccc ggcacggtta cctatccgcg cccaccggac tttaccggca
    14101 cggcgtttct tgaacagttg ctgattatgc tgacgcccga tcccgccgcg ttaaaagttg
    14161 ccccggataa agccactttc gcccgcgtca ctgcgccctt gtggcaatat cttgacgcgc
    14221 tacatcccta tttatggcgc aaaggaaagg atttcccgcc ttcgcccgcg cggatggatg
    14281 ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt taaccctgct catgcccagc
    14341 aaaaaatcgc cagcggcgat ttgcctgcaa gcagttacag ttttggtttt aaccagggga
    14401 tgattggcaa cgtgcatttc gtcaccattc ctgctaacgc aaatgccagt gctgcggcga
    14461 aggtggttgc caacttcctg ctctcaccca atgcgcaact gcgtaaagcg gatcccgctg
    14521 tctggggcga tccttctgtt ctcgatccgc aaaaactgcc tgatgggcag cgcgaaacat
    14581 tgcaatcaag aatgccgcaa gatctgccgc cggtactggc tgaaccgcac gcaggttggg
    14641 taaatgcgct ggaacaagaa tggctacacc gttacggtac gcattaatct ttttgctgtg
    14701 ggcgatggtg gcggtgattt atgcaccact gatcccggca gctctcacgc tgatatcgcc
    14761 tgctttgtcg ttgacacact ggcaggcgtt atttgccgat ccacagttac cgcaggcatt
    14821 actggcaacg ctggtgtcga caaccatcgc ggcggtcggg gcgttgttga ttgccctgct
    14881 ggtgattgtg gcgctgtggc cttggccgaa atggcagcgt atgtgtgccc gtctgccgtg
    14941 gctgctcgcc attccccatg tggcttttgc caccagcgcc cttctgctct ttgctgacgg
    15001 agggctgctt tatgactatt tcccgtattt cactccgcta atggacaaat tgggtattgg
    15061 gctgggcctc acccttgcag tgaaagaaag cgcatttctg ctgtggatct tagcggcagt
    15121 attgagcgaa aaacggctgt tgcagcaggt cattgtgctg gattcgctgg gctacagccg
    15181 ctggcaatgc ctgaactggc tgctcttgcc ctccgtcgca cctgcactgg caatggcgat
    15241 gttggcgatt gttgcctggt cgctttcggt cgtggatgtg gcgattattc ttgggccagg
    15301 taatccaccg acgctggcag taattagctg gcagtggtta actcagggcg acgcggatca
    15361 acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg ttactcgccg cctacgtttt
    15421 gctgagctat ctgctatggc gcagctggcg gcgcactatt ccccgcgtag atggcattcg
    15481 caagcctgcc acacctttat taccaggcac tacgctggcg agttttttac ccttaaccgg
    15541 tgtgctgtgt gtggttctgc tggcgatcct cgcggatcag tcgacgatca atagtgaagc
    15601 actcatcaac agcctgacaa tggggctggt ggcgacattc atcgctttgc tcctgttact
    15661 gctgtggctg gaatgggggc cacagcgtcg ccagttgtgg ctctggttgc ccactctatt
    15721 gcccgctctg ccactggtgg cgggccagta cacgctggcg ctatggctga acctggatgg
    15781 aagctggacg gcggtggtct gggggcatct gctgtgggta atgccgtgga tgctgtttat
    15841 cctgcaaccc gcctggcagc gcattgattc acggttaatt ttgattgcgc aaacactgag
    15901 ctggtcgcgg gccaaaatct tcttttacgt gaaatgtccg cttatgctac gccctacgct
    15961 gattgccttc gcggtgggat tttcagtcag tattgcgcag tatatgccga cgctgtggct
    16021 gggcgcgggg cgttttccga cgctcaccac tgaggcggtg gcattaagca gcggcggcag
    16081 caatggtatt ttcgccgccc aggctttatg gcaactgcta ttaccgctta ttatttttgc
    16141 cctgaccgcg ttagtcgcaa aatgggtagg ttatgtcaga caaggactcc gctaatgctc
    16201 tgcgtgaaaa atgtttcgct acgtttacca gaaagccgct tgctgacaaa cgttaacttt
    16261 acggtggata aaggtgacat tgtcacgtta atggggccgt ctggctgtgg aaaatccact
    16321 ctgttttcat ggatgattgg tgcactggct ggacagtttt cttgtacagg tgagctatgg
    16381 ctcaatgagc aacggattga catgctaccc accgcacagc gtcagattgg cattcttttc
    16441 caggatgcac tgttatttga ccagttcagc gtcgggcaaa atttactgct ggcgctaccg
    16501 tcgacgctca aggggactgc caggcgcaat gccgtgaaag acgcgcttga tcgagcggga
    16561 ctggctgaaa cttatcacca ggaccctgcg acattatcag gcggtcagcg agcgcgggtg
    16621 gccctgctgc gcgcccttct cgctcagcca aaagcgttac tcctggatga gccattcagc
    16681 cgtcttgatg tggctctgcg cgataatttt cgccagtggg tgttcagcga agttcgcgaa
    16741 ctggcgatcc ccgtcgttca ggtaacgcac gatctccagg atgttcctgc tgatagttct
    16801 gttctggata tggagcagtg gtcagaaaat tacaacaaac tgcgataacg caaagttttt
    16861 ctcaatgcgt cagttcagaa tggcgcactc aaaactacaa tgtcgggatt ttcgatgaaa
    16921 cgtgtttctc aaatgaccgc gctggcaatg gctttagggc tggcttgcgc ttcttcgtgg
    16981 gccgctgaac tggcgaagcc tcttacactt gaccagcttc aacaacaaaa tggcaaagcg
    17041 atagatactc gccccagcgc gttttataac ggctggccac aaaccttaaa tggcccttct
    17101 ggtcatgaac ctgccgcctt aaacctctct gccagctggc tcgacaaaat gagcaccgaa
    17161 cagctcaacg cgtggatcaa gcaacataac ctgaaagccg atgccccggt ggcgctgtac
    17221 ggtaatgaca aagatgtcga cgccgtcaaa acgcgactgc aaaaagcagg ttttacgcat
    17281 atctccattc tgagtgacgc gctaagcgaa ccttcccgtc tacaaaaact gccgcacttt
    17341 gagcagctgg tttatccgca atggctgcac gacctgcaac aaggtaaaga ggttacggcg
    17401 aaacctgccg gtgactggaa agtcattgaa gcggcctggg gcgctcctaa gctttacctt
    17461 atcagccata ttcccggtgc tgactacatc gataccaacg aagtggaaag cgaaccgctg
    17521 tggaacaaag tttctgatga acaactaaaa gcgatgctgg caaaacacgg cattcgccat
    17581 gacaccacgg tcattctgta cgggcgtgac gtatacgctg gagcgcgtgt ggcgcaaatt
    17641 atgctctacg caggcgtgaa agatgtgcgt ctgctggatg gcggctggca aacctggtcc
    17701 gacgcgggac tgcctgttga gcgaggaacg ccaccgaaag tgaaagcgga accggatttc
    17761 ggcgtgaaga tcccggcaca accgcagttg atgctcgata tggaacaggc ccgtggactg
    17821 ctgcatcgcc aggatgcatc gctggtgagc attcgttcgt ggccagaatt tatcggtacg
    17881 accagcggtt acagctatat taaaccaaaa ggtgaaatag ccggagcacg ttggggacac
    17941 gctggtagcg actcgacgca tatggaagat tttcataacc cggatggcac catgcgcagc
    18001 gccgatgata ttaccgctat gtggaaagcg tggaatatca aaccagagca gcaagtttca
    18061 ttctactgcg gcaccggctg gcgcgcgtcc gaaaccttta tgtacgcacg cgccatgggt
    18121 tggaagaatg tttccgtgta tgacggcggc tggtacgaat ggagcagcga tccaaaaaat
    18181 ccggtagcaa ccggtgaacg cggcccggac agtagcaaat aacattgcaa ttactgacgc
    18241 tggtgcgact tcagcgtcag atacccactc cacacccgcg taaaggtcgt catccagcac
    18301 aacgcaccga agatccacgc aaaccacgca aaccatgcag ggaataaaca tcccagcaca
    18361 aacagtaaga tcgtttctgt gccttcggtt aacccgccca gataataaaa cgatttatgc
    18421 gcataaccgg gattatcaat ctgatgtttc gccgccagcg cagcaaaagc gagaaaactg
    18481 ctgcctgtgc cgataaacgc aaacagcagc cagccgcctg ccagggcgtt ttgctgcggt
    18541 gcggcgagga taaacccaaa aggtaccagc gcgtaaaaaa gaaaatcgag agaaatatca
    18601 agaaagccgc ccgcgtcggt aagccctctg cgccgcgcca gcgcaccgtc cagaccatca
    18661 agcagcctgt tcaacaaaat gacgacaagc gccgccagat accagcccag cgccagaaaa
    18721 ggcagtgcca gcacgccgat ggcaaatccc actaatgtta aaccatctgg cgtaatgccc
    18781 ggtttatcaa gaacccgcac gcactgatgc aacaacggtt taatccgggg atgaagatgg
    18841 cggtctagca caggcactcc ttaaatataa agcctttctg attgagcaac agtgcggata
    18901 ttatggcatt tttcgcttat ctgcccgtgt gtaatttatg aaaatgattg aagttgttgc
    18961 cgccatcatt gaacgtgatg gcaaaatttt actcgcgcaa cgccccgccc agagcgatca
    19021 ggcgggatta tgggagtttg ccggtggtaa agtcgagccg gatgaaagcc agcggcaggc
    19081 gctggtgcgt gagttacgcg aagaactggg catcgaagca actgtgggtg agtatgttac
    19141 cagccatcag cgagaagttt cgggccggat tatccatctt catgcctggc acgtacccga
    19201 cttccacggg acgttacagg cacatgaaca tcaggcgctg gtctggtgct cacctgaaga
    19261 ggcgctgcaa tatccgctgg cccctgctga cattccatta ttagaggcgt ttatggcttt
    19321 acgcgccgcc agaccagcgg attagtgcta agggttttgt catcccgctg gcattgcagc
    19381 agtattccct cggctttaat taccgcgcct tcagaataat ttttatcctg ataaacgcag
    19441 cactgagtac agggctgcgc tgactgcccg cctgaactga acacttctgg cggtacgttt
    19501 acctccacgt ccggacgata atgcgggtta gccagtgcaa ttaatggaaa tgctaatact
    19561 acggcgaaca atgctcgact cacagggaac tccttaacgt tattgtctct gctactgata
    19621 acggtagccg ggtggcaaaa ctttagcgtc tgagttatcg catttggtta tgagattact
    19681 ctcgttatta atttgctttc ctgggtcatt tttttcttgc ttaccgtcac attcttgatg
    19741 gtatagtcga aaactgcaaa agcacatcac ataaacaaca taagcacaat cgtattaata
    19801 tataagggtt ttatatctat ggatcagaca tattctctgg agtcattcct caaccatgtc
    19861 caaaagcgcg acccgaatca aaccgagttc gcgcaagccg ttcgtgaagt aatgaccaca
    19921 ctctggcctt ttcttgaaca aaatccaaaa tatcgccaga tgtcattact ggagcgtctg
    19981 gttgaaccgg agcgcgtgat ccagtttcgc gtggtatggg ttgatgatcg caatcaggta
    20041 caggtcaacc gtgcatggcg tgtgcagttc agctctgcca tcggcccgta caaaggcggt
    20101 atgcgcttcc atccgtcagt taacctctcc attcttaaat tcctcggctt cgaacaaacc
    20161 ttcaaaaatg ccctgactac cctgccgatg ggcggtggta aaggcggcag cgatttcgat
    20221 ccgaaaggaa aaagcgaagg tgaagtgatg cgtttttgcc aggcgctgat gactgaactg
    20281 tatcgccacc tgggcgcgga taccgacgtt ccggcaggtg atatcggggt tggtggtcgt
    20341 gaagtcggct ttatggcggg gatgatgaaa aagctctcca acaataccgc ctgcgtcttc
    20401 accggtaagg gcctttcatt tggcggcagt cttattcgcc cggaagctac cggctacggt
    20461 ctggtttatt tcacagaagc aatgctaaaa cgccacggta tgggttttga agggatgcgc
    20521 gtttccgttt ctggctccgg caacgtcgcc cagtacgcta tcgaaaaagc gatggaattt
    20581 ggtgctcgtg tgatcactgc gtcagactcc agcggcactg tagttgatga aagcggattc
    20641 acgaaagaga aactggcacg tcttatcgaa atcaaagcca gccgcgatgg tcgagtggca
    20701 gattatgcca aagaatttgg tctggtctat ctcgaacgcc aacagccgtg gtctgtaccg
    20761 gttgatatcg ccctgccttg cgccacccag aacgaactgg atgttgacgc cgcgcatcag
    20821 cttatcgcca atggcgttaa agccgtcgcc gaaggggcaa atatgccgac caccatcgaa
    20881 gcgactgaac tgttccagca ggcaggcgta ctgtttgcac cgggtaaagc ggctaatgct
    20941 ggcggtgtag caacgtcagg ccttgaaatg gcgcaaaacg ctgcgcgcct gggctggaaa
    21001 gccgagaaag ttgacgcacg tttgcatcac atcatgctgg atatccacca tgcctgtgtt
    21061 gagcatggtg gtgaaggtga gcaaaccaac tacgtgcagg gcgcgaacat tgccggtttt
    21121 gtgaaggttg ccgatgcgat gctggcgcag ggtgtgattt aagttgtaaa tgcctgatgg
    21181 cgctacgctt atcaggccta caaatgggca caattcattg cagttacgct ctaatgtagg
    21241 ccgggcaagc gcagcgcccc cggcaaaatt tcaggcgttt atgagtattt aacggatgat
    21301 gctccccacg gaacatttct tatgggccaa cggcatttct tactgtagtg ctcccaaaac
    21361 tgcttgtcgt aacgataaca cgcttcaagt tcagcatccg ttaactttct gcgatagcag
    21421 cagatatgcc agtaaagaaa ccccattaga ctattttttt gataatcttc ttcgctttcg
    21481 aacaactcgt gcgcctttcg agaagcaagc attatataat gccaggccag ttcttcttca
    21541 attgtcccgt tttgaaaagc tgtgcttgat atcgaggtca tccatgataa ttccgccgcc
    21601 catattagct tcgccgagga tttaccggag cgattattag cgcaatcaga gatatagtct
    21661 gagggaaaaa cagcaaattt attcaacaag gcgataacct gctctggggc ttcctccatg
    21721 tttgctttaa aggtattggc tccatggtcg ccagaaagaa aatactccat taaggcacaa
    21781 taactttcgc tatcttcgat accccattga tcctctaaag actcgcgtct tttacttatg
    21841 atatcgatcg agtcaaaggg aagcacatga tattggaagg tatctttgcc aggttcaggc
    21901 tttcgcggcc agaactccag cgtttcagac cattgcttat gatagaaccg ataaggtgcg
    21961 atcaattgta gcgcctgtaa cttctcgata ctgagcggct caataccttt agcctgataa
    22021 taatgcagtt gttctttttt tgctttaaaa ccggcccgaa caataagccc catcataatt
    22081 aatagataaa gaaaagagca tcccgcggta atcaggcctc tttcattcaa accgttggat
    22141 gttatcgctg cgaacacaaa cattacagcg acaacacatg ttaaataaaa cccccactta
    22201 caaagcagca tggccttatt ttctttaatc atccgttcaa aattactatt aaatatttcc
    22261 cagccattaa aagaatactt ctcgctccca ggatggtttt gtaataaaac ttttttcatc
    22321 acattccctg ttattacata ctttagaggc gataatttta tccatgcaaa aaaaatatcc
    22381 aacgaatttt cttgatccgc tggatatttc agaatatgac tcgaatagca cgaaagattc
    22441 actcgcttac gctatcgccc cgcttccgac ttcatctgct ggcggacttt ttttcgcact
    22501 acgtttacgc ggtgcagcct ttttcttatc agcactgcca ccactgcccg gagccacaat
    22561 gccgcgaaac tgccgcaccg gcgtacgttt ggcttgatca ataagctgat atagcgtccc
    22621 caccagcggc tgcataaagt cctgatagcg acactgcttt tcgctgattt gcgtcagcac
    22681 cgattcccag tgcgcggtca tgtccggtcg cgtcgccatt tctggcagcg aatggaataa
    22741 cgcttttccg gcgtcggtag agtggatata gcgccctttt ttggtcagaa aaccacgctt
    22801 gaacagcagt tcaataatcc cggcacgggt tgcttctgtc cccagaccat cggtcgcacg
    22861 aaggatcttt ttcagatctt tgtcctgcac aaagcgcgcg atcccggtca tcgccgaaag
    22921 cagtgttgca tcggtaaaat ggcgcggcgg ctgggtttgc cgctctacca cttcaccttt
    22981 ttcacacagc aactcatcgc ctttcgccac cacaggcaat ggcgtaccgt cgttttcttc
    23041 atcgcgctct ttgctgccta acagcgtgcg ccagcctgct tcagcaagaa aacgggcttt
    23101 agcgacaaat ttgcctttgg caatgtccag ttcgataaca cacttgcgga acaccgcatc
    23161 cgggcagaac tgcatcaggt attgacgggc aatcaggtta tagaccttcg cttcgttctc
    23221 cgtcaggttg atcgcagaac tccgtgcggt cgggatgatg gcgtggtgcg catcgacctt
    23281 tttgtcatcc caacagcggt tgcgtatatc tggatctacc actgactgcg gcaacagatc
    23341 cggtgcatga acgctgatgg cattcatcac cgcgtggcgt ccggcaaaat gttcttctgg
    23401 caaatagcga caatcggaac gcggataagt gattagcttg tgcgtttcgt acagcttctg
    23461 gcagatatca agcacgttct gcgcgctcag accgaagcgt tttgccgctt caatctgtaa
    23521 cgccgaaagc gaaaacggca gcggcgcgga ttctgattcc cgtttatcgt tatagctggt
    23581 gacaatagcc ggttgaccac taatgcggtt aaccacatgc tccgccagtg gacgatgtaa
    23641 caagcgccct tcttcatcct ggtacggttc acacgcttcg ctcggttgcc agatagcggt
    23701 aaaccgctca tcggcaggtg tcacgatatg cgctttgact tcaaagaagt ctttcgccac
    23761 gaagttttca atctcttcat cgcggcgcac caccagcccg agcacgggtg tctgcacgcg
    23821 tcccacggaa agtacgccct gataaccggc attgcgaccg agaatggtat acgcacgggt
    23881 catattgatg ccgtacagcc agtcggcacg cgctcgcgcc agcgcagaaa cgcacagcgg
    23941 tacaaactca ctgttggaac gaagacggtc gatcgcccgc tcaaccgcct gcgggttcag
    24001 gtcgtttatc aagcaacgct gcacctgctg gcgcttttcc ggtgccagtt gcagatagtc
    24061 cagcacttca tccaccagca attgcccttc acgatccggg tccccggcgt gaacgatttc
    24121 gctggcttca tgcaggaagc gtttgatgac gttaagttgt ttggtcacgg agggtcgggg
    24181 ctgtaattgc cacttttccg ggacaatcgg caaatccgca agattccagc gcgcatagcg
    24241 gctgtcgtag gcgtctggct gcgcctgctc aagcaggtga ccgatacacc aggtcaccac
    24301 ctgaccatta ccgcactcga taaagccatc gcctttacgg tgcggtttgg gcaggacatc
    24361 agcaatggcg cgcgccagac tcggtttttc ggcaataaac aaccgcattg aattaatgga
    24421 tctcaaccat ggcacgaccg ccgcgcgctg gcaccagttc gccaatcgcc gtcagttcaa
    24481 tgccaaactc ggcggctgta gttttaacct cattttctgc ttccggcatg accgccagca
    24541 gcaaaccgcc agaagtttgc ggatcgcaca gcagatcgcg cacttcacgc ggcatttcac
    24601 ccatcagatg accgtagctg gcaaagttac gttcggtgcc gccaggcact gcgcccaact
    24661 taatgtactc ttcaaccccc ggcaattttg ggatcgcgtc atagtcaacg cgcgcctgca
    24721 cacctgcgcc ctgacacatt tcgctcaaat ggcccagcag accaaaacca gtaacgtcgg
    24781 tcatcgcttt tacgccttcg atgttggcaa aggacgcacc tgcgatgttc atccggcaca
    24841 tcacttccgt cgccagtccc tgatgttctg gtttcaacag tgattttttc tcagccgtgg
    24901 taagaacgcc gatccccagc ggtttcgtca ggaacagttt gcatccggct tgtgcggtac
    24961 tgtttttctt cacccgctcg gtcggtacga tccccgttac cgccagacca aaaatcggct
    25021 ccggcgcatc gatggaatga ccgccagcca gcgcaatacc cgcctgacgg catgcatagc
    25081 gtccaccttc ggttacttcg cgggcaattt ctggggaaag tttgttaatc ggccagccga
    25141 ggatcgcaat cgccataatc ggtttgcccc ccatcgcgaa gatatcgctg atggcgttag
    25201 tcgccgcaat gcggccaaaa tcgaaaggat tatcaacgat cggcataaag aagtcggtgg
    25261 tactgataac gctggtgcca ttgcccagat cgtacaccgc cgcatcgtcg cgggtctcat
    25321 tacccacaag caaattcgga tcaacaaact tcgcctgctc actgtgcagg atggtttcca
    25381 acacttttgg ggaaatttta cagccgcaac cagctccgtg gctgtattgg gtcaaacgaa
    25441 tcgagttctc gctcatggac atctcctgtc aatgcaatcc gggtatggta accctcattc
    25501 cgtgaagtga taagtgagag tgtctgaatt cctgcgcctt tgctcacaat ccagacagtt
    25561 tcgcgacaat tatcagaaat aagttacaaa cggcgtcggg tccgggacgt taatcgacgt
    25621 agatgctttc aactgcggcg tgccgaggta gagaaaaccg acaattttat cctgctcacg
    25681 gcaaccgaat gcttcacgca ctaccggact ttcagttaat gcgccactgc gccagatgcc
    25741 gccaaatcct tgggcaacgg ctgccatttg catcgccatg accgcgcatc cggcagacat
    25801 ttcctgttcc catcgcggga ctttatgatt ttcttcgcat ttcgccacca ccgtgatgat
    25861 gagcggtgcg cggaacggcg cattacgggc tttgtcgata gctttgtcat cactaccggc
    25921 agcaatcgcc ccctgttcca gtacggcgct gaaacgctcg cgcccttccc cttcaatcac
    25981 aaaaaaatgc cacggttgca tggacttatg gtccggcgca cgcatacccg cacgcaggat
    26041 gttttgcagt tgttcacccg ttggcgcggg ttcagccagg cgggaggcgc tacggcgatt
    26101 gatcaatagt tcgagtgcat ccatttgatt aactcctgtc gtgatattta ttcacaaaat
    26161 taacacgaga gtggattttg ttacagcaca gtccgcaatt cctgctgaca agtgccggtt
    26221 gggtcattac gataaccaca tctattgcgc ctgtgacagg tgtgacctta agttgggaga
    26281 atacatgcga accctttggc gatttattgc cggatttttt aaatggacgt ggcgtctgct
    26341 gaatttcgtc cgtgaaatgg tacttaacct gttctttatt ttcctcgtac tggttggtgt
    26401 ggggatctgg atgcaggtca gtggtggtga ttcgaaagaa acggccagtc gtggcgcact
    26461 gctgctggac atttctggtg tgatcgtcga taaacccgac agttctcagc ggtttagtaa
    26521 attaagccgc cagctgcttg gtgccagttc cgatcgtctg caggaaaact cactgtttga
    26581 tatcgtcaac accattcgcc aggcgaagga cgaccgcaat atcaccggta tcgtgatgga
    26641 tctgaaaaac ttcgcaggcg gcgaccagcc gtctatgcag tacatcggca aagccctgaa
    26701 agagtttcgt gacagcggga aaccggttta tgccgttggc gagaactaca gccaggggca
    26761 atattatctc gccagtttcg ccaataaaat ttggctgtca ccgcaaggcg tggttgatct
    26821 gcacggtttt gctaccaacg gtctgtacta caaatcgttg ctggataagc tgaaagtttc
    26881 cacccatgtg ttccgcgtgg gtacgtataa atctgccgtt gaaccgttta ttcgtgatga
    26941 tatgtcaccg gcagcccgcg aagctgacag ccgctggatt ggtgagctgt ggcaaaacta
    27001 tctgaatact gttgccgcta accggcagat ccctgctcag caggtattcc ctggcgcgca
    27061 agggttgctt gagggtttaa ccaaaaccgg tggcgatacc gcgaaatatg cactggaaaa
    27121 caagctggtc gatgcactgg catcgagtgc cgaaatcgaa aaagctctga ccaaagagtt
    27181 cggctggagt aagactgata aaaattatcg cgccatcagt tattacgatt acgcattgaa
    27241 aacgccggca gataccggtg acagcatcgg tgtcgtcttc gctaatggcg caattatgga
    27301 tggcgaggaa actcagggga atgttggcgg tgataccact gcggcacaaa tccgcgacgc
    27361 tcgccttgac ccgaaagtga aagcgattgt cctgcgtgtt aatagcccag gcggcagcgt
    27421 taccgcgtct gaagtgattc gcgctgaact ggcagcagcc cgggcagcgg gtaagcctgt
    27481 ggttgtatcg atgggcggca tggcggcatc tggtggttac tggatttcca cgccagctaa
    27541 ttacattgtg gctaacccca gcaccctgac cggttctatc ggtatcttcg gcgtgatcac
    27601 caccgtagaa aatagtctgg attcgattgg tgttcatact gatggtgtct caacttcacc
    27661 gctggcggat gtttctatca ccagggcact gccgccggaa gcgcagcaga tgatgcaatt
    27721 aagcattgag aatggctata aacgctttat cacgctggtt gctgatgcgc gtcattcgac
    27781 gccggagcaa attgataaaa tcgcccaggg ccacgtctgg accggtcagg atgcaaaagc
    27841 taacgggctg gtcgatagtc tcggggattt cgatgatgcg gttgccaaag cagcagagct
    27901 ggcaaaagtg aaacagtggc atctggaata ctacgttgat gaaccgacct tcttcgacaa
    27961 agtgatggac aacatgtctg gttctgtccg ggcaatgttg ccagatgcgt tccaggccat
    28021 gttacctgca ccgcttgcct cggtagcctc taccgttaaa agtgaaagcg acaagctggc
    28081 cgcgtttaac gacccacaaa accgttatgc gttttgcctg acctgcgcca acgtgcgtta
    28141 agtcttgtac tgagtggccg acagatcgtc ggccacatta ttttttacct cgacgaatcc
    28201 tcttcccgct gtctcgaccc atatttcctt atccgcatag tatcaggtgc gctcctcctg
    28261 cctcacgtat atacttttgc tctttcgata tcattcatat caatatcatg caaaagaaat
    28321 caatttacgt cgcctacacg ggcgggacca tcgggatgca gcgttccgag cagggttata
    28381 ttccggtgtc aggtcatcta caacgccaac tggcgctgat gccggaattc catcgcccgg
    28441 agatgccaga tttcaccatt catgaatata cgccgttgat ggattcttct gatatgacgc
    28501 cggaagactg gcagcatatt gctgaagata ttaaagcgca ctatgacgac tatgatggtt
    28561 ttgtcattct acacggtacc gacacgatgg cgtataccgc ctctgcgctg tcgttcatgc
    28621 tcgagaatct cggtaaaccg gtcattgtga cagggtcaca aatcccgctg gctgagttac
    28681 gctctgacgg acaaattaat ctgctgaatg cgttgtacgt tgcggcgaat tatccgatca
    28741 acgaagtaac gctctttttc aataaccgat tgtatcgcgg caaccgcact accaaagccc
    28801 atgccgatgg ttttgatgcg tttgcctctc caaaccttcc tccgttactg gaagctggta
    28861 tccatattcg tcgtttgaat acgccacccg ccccgcacgg tgaaggggaa ttaatcgttc
    28921 atccaatcac gccacaacca attggcgtag tgacgattta tccagggatt tctgctgacg
    28981 tcgtgcgcaa ttttctgcgc caaccggtga aagcattgat tctgcgctcc tatggcgtgg
    29041 gtaatgcgcc acaaaacaaa gccttcctgc aggaattaca agaagccagc gatcgcggta
    29101 ttgtggtggt caacctgaca caatgtatgt ccggtaaagt gaatatgggt ggttatgcca
    29161 ccggtaacgc cctcgcccat gccggcgtta ttggcggtgc agatatgact gtagaagcca
    29221 cgctaaccaa actgcattac ctgctgagcc aggaactgga tactgaaacc attcgcaagg
    29281 ccatgagcca aaacctgcgc ggcgaactga cgccggatga ttaaggagac tgcaatgccc
    29341 catcgcgccc tgttactggt cgatttacaa aatgatttct gtgctggtgg cgcgctcgcc
    29401 gtgccggaag gtgacagtac ggtagatgtc gctaaccgcc tgattgactg gtgccagtcg
    29461 cgcggtgaag cggttatcgc cagtcaggac tggcacccgg cgaatcacgg cagttttgcc
    29521 agtcagcacg gtgtagagcc ttatacgcca ggccaactcg acggtttgcc acaaaccttc
    29581 tggccagatc actgtgtgca gaacagtgaa ggcgcacaat tacatccgtt actgaaccaa
    29641 aaagcgatcg cagcggtgtt ccataaaggc gaaaatcctt tagttgacag ttacagtgcc
    29701 ttttttgata acggccgtcg gcagaaaacc tctctcgatg actggttacg cgatcatgaa
    29761 atcgatgaat tgatcgttat gggcctggct actgactatt gcgtgaagtt taccgtgctg
    29821 gacgcgttac agttaggtta taaggtaaac gtgattaccg atggttgtcg tggcgtgaat
    29881 atccagcccc aggacagtgc gcacgcgttt atggagatgt cagcagctgg ggcaacgcta
    29941 tatacgctgg cagactggga agagacgcag gggtaatatt agttgaattc atctctggcc
    30001 tacatttcct caatgacatt gtaggccaga taatacttgt cagtgttgca tttaacctac
    30061 ttgcagctaa aacacgacgt cctaacaagt atatttgccc tcccttaata acaggcttaa
    30121 atactagata actcattcac ttaaacatag caataggctt tgctgagata ccaaaatctt
    30181 cttttaactg ctgtttgctc ttcatgacca tctgaccatt tgtgtcaatt gtcatatgct
    30241 gtgcttcggt attatggcgg gcttgccaca gcatcaccag ttgcaggcaa ttctcttttt
    30301 gttcctcggt caacgcaacg ccatcaggcc atttccccag ttcaacggcg gtcgacaaac
    30361 gctggtatac ctcaggcgtc atgctgttga taatgtcatc aagattcata atttttccgc
    30421 tcctgtggaa taagttgctg aatcgttttt tcaaccgttg atttcttcgc cgttttcgcc
    30481 atcagtgaag cgtaaagaag cagagttaac acaataacgt tcgcctgttg gttgcggccc
    30541 gtcgggaaag acatgcccca gatgggcatc acagttaccg caacgaattt ctatgcgctg
    30601 cattccatgt gacaagtctt tgatataacg aatggattct tcactcaccg gttcgtagaa
    30661 actgggccag ccacagccgg aatcatactt ggtttcggaa tgaaacagcg gggcatcgca
    30721 gatcaaacag tgatatacgc cgtcacgctt gttatgcagt aaacgacccg taaatggcgg
    30781 ttctgtccca tgattctgcg tcacgtaaaa ctgcatctcg gacaaatttt ttttcagttc
    30841 ttctgccgaa ggtttattag ccatttgctc acatctcact ttaatcgtgc tcacattacg
    30901 tgactgattc taacaaaaca ttaacaccaa ctggcaaaat tttgtcctaa acttgatctc
    30961 gacgaaatgg ctgcacctaa atcgtgatga aaatcacatt tttatcgtaa ttgcccttta
    31021 aaattcgggg cgccgacccc atgtggtctc aagcccaaag gaagagtgag gcgagtcagt
    31081 cgcgtaatgc ttaggcacag gattgatttg tcgcaatgat tgacacgatt ccgcttgacg
    31141 ctgcgtaagg tttttgtaat tttacaggca accttttatt cactaacaaa tagctggtgg
    31201 aatatatgac tatcaaagta ggtatcaacg gttttggccg tatcggtcgc attgttttcc
    31261 gtgctgctca gaaacgttct gacatcgaga tcgttgcaat caacgacctg ttagacgctg
    31321 attacatggc atacatgctg aaatatgact ccactcacgg ccgtttcgac ggtaccgttg
    31381 aagtgaaaga cggtcatctg atcgttaacg gtaaaaaaat ccgtgttacc gctgaacgtg
    31441 atccggctaa cctgaaatgg gacgaagttg gtgttgacgt tgtcgctgaa gcaactggtc
    31501 tgttcctgac tgacgaaact gctcgtaaac acatcaccgc tggtgcgaag aaagtggtta
    31561 tgactggtcc gtctaaagac aacactccga tgttcgttaa aggcgctaac ttcgacaaat
    31621 atgctggcca ggacatcgtt tccaacgctt cctgcaccac caactgcctg gctccgctgg
    31681 ctaaagttat caacgataac ttcggcatca tcgaaggtct gatgaccacc gttcacgcta
    31741 ctaccgctac tcagaaaacc gttgatggcc cgtctcacaa agactggcgc ggcggccgcg
    31801 gcgcttccca gaacatcatc ccgtcctcta ccggtgctgc taaagctgta ggtaaagtac
    31861 tgccagaact gaatggcaaa ctgactggta tggcgttccg cgttccgacc ccgaacgtat
    31921 ccgtagttga cctgaccgtt cgtctggaaa aagctgcaac ttacgagcag atcaaagctg
    31981 ccgttaaagc tgctgctgaa ggcgaaatga aaggcgttct gggctacacc gaagatgacg
    32041 tagtatctac cgatttcaac ggcgaagttt gcacttccgt gttcgatgct aaagctggta
    32101 tcgctctgaa cgacaacttc gtgaaactgg tatcttggta cgacaacgaa accggttact
    32161 ccaacaaagt tctggacctg atcgctcaca tctccaaata agttgagatg acactgtgat
    32221 ctaaaaagag cgacttcggt cgctcttttt tttacctgat aaaatgaagt taaaggactg
    32281 cgtcatgatt aagaaaattt ttgcccttcc ggtcatcgaa caaatctccc ctgtcctctc
    32341 ccgtcgtaaa ctggatgaac tggacctcat tgtggtcgat catccccagg taaaagcctc
    32401 ttttgcatta cagggcgcac accttctctc gtggaaacct gcgggtgaag aagaagttct
    32461 gtggttgagc aacaacacac cgttcaaaaa tggcgtcgct attcgcggtg gcgtaccggt
    32521 ttgctggccg tggtttggtc cggcggcaca acaaggtctg cctgcgcacg gttttgcccg
    32581 caacctgccg tggacgctga aatcacatcg tgaagatgct gatggcgtag cgctgacttt
    32641 tgaattgacg caaagcgaag agacgaaaaa attctggccg cacgacttta cgctgttagc
    32701 gcatttccgc gtgggtaaaa cctgtgaaat cgatcttgaa tcacatggcg aatttgaaac
    32761 cacctctgcc ctgcatacct actttaacgt gggtgatatc gctaaggtaa gcgtcagtgg
    32821 gctgggcgat cgcttcattg ataaagtaaa tgacgcgaaa gaagatgtac tgaccgacgg
    32881 tattcagacc ttccctgacc gtaccgatcg cgtgtatctg aatccgcaag attgcagcgt
    32941 gattaatgat gaagcgctga atcgtattat cgccgtaggc caccagcatc atctgaacgt
    33001 tgtcggctgg aacccgggac cggcgctttc agttagcatg ggcgacatgc cggatgatgg
    33061 ctacaaaaca tttgtttgtg tagaaacggc ttacgcttca gaaacgcaaa aagtgaccaa
    33121 agagaaacct gcacatctgg cgcaatccat tcgcgttgcg aaacgttaat ttgcgttaat
    33181 gttgtgtgcc gggtgcgatg catccggcac gtatcaacat cacaccatat ccagcgcagt
    33241 ttttcctttt ggtgccggat acgctttatc cagcatggtt aattccgctg acgaaagttc
    33301 gacctcaagc acagccgcat tctgttggac atgggcaatc gtggccgctt ttggaatcgc
    33361 catcacaccc tgatggctga ttacccacgc caacaatact tgtgccgcgc tgatattgtg
    33421 agcatgtgca atttcgttga ctaccgcgtt ttttaacagt ccattgcgca accgcccggc
    33481 ctgggctaat ggactgtaag ccatcaccgg catctgctgt tgctggcacc aggggagtag
    33541 atcgtactca atccctcgtg aaccgagatg gtaaagcacc tgattagtgg cacactgatt
    33601 tccccccggc agctgccaga gttcctgcat atcagcatga tcaaggttag aaacgcccca
    33661 gcggcggatt tttccctggg cgatcaattt ttccatcgct gccacagtct cttcaaaagc
    33721 gaaactgcca gaccagtgta ataagtagag atcgagataa tcagtattga gacggcgtaa
    33781 actggcttcg catgcattta tcgctttttg cccgccagca ttccacggat agactttaga
    33841 gacgagaaag acgttatcgc gcagaccggt taatgcttcc ccaaccacct tttcggcacc
    33901 gccatcggca tacatttcgg cggtatcaat gagggttaaa ccgagctcaa tgcccgcgcg
    33961 tagtgcagca acttctgttt tgcgctgact ggcatcttcg cccatatacc atgttccctg
    34021 ccctatggct ggcagtgaga catcgccact aaattgaatc attttttgtt gcattgtttc
    34081 ctcccaggta ttgcaccacc gtaatgcaaa acagggcgtg acgccctgtt tttatgcaca
    34141 aaatgccctg gaaagatgca ttatcagaat ttgtaggtga tcccggtaga aatcaggcca
    34201 gtccaggatt tatccaccat cgggctgtca gtaacttcat cagacagacg ggtatagcgc
    34261 gcggtaccgt aaacactcca gtcgccgagg aagttgtagc tggcgctcag ctccaggtaa
    34321 gggctccagc tgtcgttcgg gttatagcca cgcagaccgc tgcgagcgga ctctttgcgc
    34381 gatacgccat aatagtattc gttctggttt tcgctgttcc actgcacacc gatacccggg
    34441 gtcacggtca ggccgccgtt ggtgtaacga tacaaccagg ccatatccca gacgatgccg
    34501 ttgctgttat ccagggtatc gccagccagg gtggtacgca ggtaaccgta ctgggtaaag
    34561 tgagcataag acagaccagc catcatggtg ctcttacggt catccaggtg acgcatttgg
    34621 tgatcgccac tgtctttcgc tttgaagtaa agcggcgacc aataagcggt aattgaaagt
    34681 ttgtccgttg cgtcattcca caggtagtaa ccaccaccta agccacggaa ccagaagtta
    34741 tcgccttcat agttgattac cggtactggg taaacatcgg tatcgtaatc tttatatggg
    34801 tgctcaacga cacctacgcc tgcgcccagg gaaaatttac cttcagcgtg cgctacgcct
    34861 gcagacgttg cgataagcac tccaagtgcc agaagtttga gtttggtcac aattaatcat
    34921 tccttaaaca aatgtttagc gggcgacaaa gtttacccgt caatacatcg aaacccaacc
    34981 tttttacgtt ttcatttttt aaagtaactg tttaattttc ctgacgcgga tgacaccgcg
    35041 cttacagcca aatgaattta gcgttactgg ctaccctggt ctttacatta attatgcaaa
    35101 atttatggat gagttgttga tatgccattg aaattaagaa agccgtgcag gcaagttttc
    35161 catttgccat ctacgcttaa ttttgaaggt gtatcaccgg gcacgttgtt ctcatcgtcg
    35221 ataaaatggc atgagagttg ctgtgtttta gcaagagacg tcgttcagtt tacctcttcc
    35281 gggagcctct actattcata tgaacggctc ttaacctgtg ctaaaaaacg aaaggacggc
    35341 ataccatgaa tatattcgat cactatcgcc agcgatatga agctgccaag gacgaagagt
    35401 tcacactgca ggagtttctt accacttgtc ggcaagatcg cagtgcttat gccaacgcgg
    35461 ctgagcggct attgatggct atcggtgagc ctgtcatggt cgatacagcc caggaaccca
    35521 gactttctcg actcttttct aaccgggtca ttgcacgtta tccggcattt gaagagtttt
    35581 acggcatgga agacgcgatt gaacagattg tctcttatct gaaacacgcg gctcaggggc
    35641 tggaagagaa gaaacaaatc ctgtatctgc tggggcctgt gggtgggggt aaatcatcgc
    35701 ttgctgagcg actgaaatca ttaatgcagc tcgtaccgat ttatgtattg agcgccaacg
    35761 gtgagcgtag cccggtcaac gatcatccgt tctgtctttt caatccgcag gaagatgctc
    35821 agattctgga aaaagagtat ggcattcctc gccgttatct cggcaccatc atgtcgccat
    35881 gggcggcaaa acgcctgcat gaatttggtg gcgatatcac taagttccgg gtagtgaagg
    35941 tctggccgtc aattctgcaa caaattgcta tcgccaaaac ggaacccggt gatgagaaca
    36001 accaggacat ctccgcgctg gttgggaaag tcgatattcg taaactcgaa cactacgcgc
    36061 agaatgaccc ggacgcctac ggctattccg gtgcgctgtg ccgcgccaac caagggatca
    36121 tggaatttgt cgagatgttt aaagcaccga taaaagtgct gcatccgctg ttaaccgcca
    36181 ctcaggaagg taactacaac gggacggaag gtatctccgc cctgccgttc aacgggatta
    36241 ttctcgccca ctcgaacgaa tccgaatggg tcactttccg taataacaaa aacaacgaag
    36301 ccttcctcga ccgtgtttac atcgtgaagg tgccgtattg cttacgcatt tccgaagaga
    36361 tcaaaatcta cgagaaattg cttaatcaca gtgaattgac tcacgccccc tgcgcccctg
    36421 gcacgctgga aacgctgtca cgtttttcca ttctttcgcg ccttaaagag ccagaaaact
    36481 ccagcattta ttcaaagatg cgggtttatg atggcgaaag tctgaaagac accgatccca
    36541 aagccaagtc gtatcaggaa tatcgtgact acgccggtgt cgatgaaggg atgaacggtc
    36601 tgtcgacgcg ttttgcgttt aagatcctct cccgcgtgtt caacttcgat catgtagaag
    36661 tggcggcaaa cccggtccat ctgttctacg tcctggaaca gcagatcgag cgcgagcagt
    36721 tcccacaaga gcaggcagaa cgctatctgg agttcctgaa aggttatctg atcccgaaat
    36781 atgccgagtt tatcggcaaa gagatccaga cggcctacct tgaatcctat tccgaatatg
    36841 ggcaaaacat tttcgaccgt tatgttacct acgcggattt ctggattcag gatcaggagt
    36901 atcgcgatcc ggataccggg cagctgtttg accgcgagtc tcttaacgcc gagctggaga
    36961 aaatcgagaa accggcgggg atcagtaatc caaaagattt ccgcaacgag attgttaact
    37021 tcgtactgcg cgccagagcg aataacagcg gacgcaatcc gaactggacc agctatgaaa
    37081 aactgcgcac ggttatcgag aagaaaatgt tctccaatac cgaggagctg ttgccggtta
    37141 tctcgtttaa cgccaaaacg tcaaccgacg agcagaagaa acacgacgac tttgtcgacc
    37201 gtatgatgga aaaaggctac acccgtaaac aggtgcgttt actgtgcgaa tggtatttgc
    37261 gcgtacgtaa atcgtcttaa caaccctggc ccggtacaca tgttaccggg cctacaacga
    37321 cagcgaaccg tgggcctgag aagcggcaac acaggcgtag catacagttg gcaaatgtag
    37381 tacggggggc atatgacctg gtttattgac cggcgtctga acggcaaaaa caaaagcatg
    37441 gtgaatcgcc agcgtttttt acgccgttat aaagcgcaaa ttaaacagtc gatctccgag
    37501 gccattaata agcgttcggt gactgacgtc gacagcggcg aatccgtatc cattcccacg
    37561 gaagatatta gcgaaccgat gtttcatcag gggcgtggcg gtctgcgcca ccgcgtgcat
    37621 ccgggcaatg accatttcgt ccagaacgac cgaattgaac gtccccaggg tggcggcgga
    37681 ggttccggta gtggtcaggg ccaggccagc caggatggtg aaggtcagga tgaatttgtc
    37741 tttcagattt cgaaagatga gtatcttgat ttgctctttg aagatttggc cttaccgaat
    37801 ctgaaacaaa accaacaacg ccagctgacc gaatataaaa cgcatcgggc gggttatacc
    37861 gctaacggcg ttccggcaaa tatcagcgtt gtgcgctcct tgcagaactc actggcgcga
    37921 cgcacagcca tgacggcagg caagcggcgg gaacttcatg cactggaaga gaatttggcc
    37981 atcatcagca acagtgaacc tgcgcagctg ctggaagagg aacgtctgcg caaagaaatt
    38041 gcagaattac gtgccaaaat tgaacgcgtc ccttttattg acaccttcga tttacgttac
    38101 aagaactacg agaagcggcc cgatccctcc agccaggcag tgatgttttg cctgatggac
    38161 gtttccggtt caatggatca atccactaaa gatatggcta agcgttttta tattctgctg
    38221 tatctgttcc tcagcagaac gtataagaac gtggaagtcg tatacatccg ccatcatacc
    38281 caggcgaaag aagtcgatga acatgagttt ttctactcgc aggaaacagg cggcaccatt
    38341 gtttccagcg ccctgaaact gatggatgag gtagtgaaag agcgttataa cccggcacag
    38401 tggaatattt acgctgcaca agcatcggac ggcgataact gggccgatga ctctccgctt
    38461 tgccatgaaa tcctggcgaa aaaattatta cctgttgttc gttattacag ctatatcgaa
    38521 attacccgtc gtgcacatca gacattgtgg cgagaatatg agcatctgca atctactttc
    38581 gacaactttg cgatgcagca catccgcgac caggatgata tttatccggt gttccgtgaa
    38641 ctgtttcata aacaaaatgc aacagctaaa gactaaaact atcagccagg tcattatcgc
    38701 ctggctgatt tttagcttac tgtaaattat
//
LOCUS       DAZGLJ010000023        36706 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000023 DAZGLJ010000000
VERSION     DAZGLJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.023"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1414
                     /locus_tag="ACHK49_001062"
     CDS             <1..1414
                     /locus_tag="ACHK49_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO9261748.1"
                     /translation="NQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPF
                     YFLFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYLARKNGTYTDLPQNVDTAEDS
                     VQPEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIETCD
                     SVNQNQQESEQNDQKTEQNSPNAQQNEPETKQEPEKACTACGQTGGGSCPDCGAVISD
                     ATYQEIFDEENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPLIKANG
                     HHNITSTSRAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGEMGPEFSKTIDLETAG
                     GVIDRDTIKWWLKQSREAQSAILTDEIPLDDALLQLREFIDENSGEFFVQVWGNGATF
                     DNVILRRSYERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGERHNALNDAR
                     YQAKYVSAIWQKLLPSQADF"
     gene            1482..1724
                     /locus_tag="ACHK49_001063"
     CDS             1482..1724
                     /locus_tag="ACHK49_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO9261749.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            1702..2721
                     /locus_tag="ACHK49_001064"
     CDS             1702..2721
                     /locus_tag="ACHK49_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9261750.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            3129..3788
                     /gene="yccA"
                     /locus_tag="ACHK49_001065"
     CDS             3129..3788
                     /gene="yccA"
                     /locus_tag="ACHK49_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO9261751.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            3879..4208
                     /gene="tusE"
                     /locus_tag="ACHK49_001066"
     CDS             3879..4208
                     /gene="tusE"
                     /locus_tag="ACHK49_001066"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO9261752.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(4205..4483)
                     /gene="yccX"
                     /locus_tag="ACHK49_001067"
     CDS             complement(4205..4483)
                     /gene="yccX"
                     /locus_tag="ACHK49_001067"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO9261753.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMLWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            4578..5768
                     /gene="rlmI"
                     /locus_tag="ACHK49_001068"
     CDS             4578..5768
                     /gene="rlmI"
                     /locus_tag="ACHK49_001068"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO9261754.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEIFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            5826..6143
                     /gene="hspQ"
                     /locus_tag="ACHK49_001069"
     CDS             5826..6143
                     /gene="hspQ"
                     /locus_tag="ACHK49_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO9261755.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(6188..6601)
                     /gene="yccU"
                     /locus_tag="ACHK49_001070"
     CDS             complement(6188..6601)
                     /gene="yccU"
                     /locus_tag="ACHK49_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO9261756.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            6774..7436
                     /gene="yccT"
                     /locus_tag="ACHK49_001071"
     CDS             6774..7436
                     /gene="yccT"
                     /locus_tag="ACHK49_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO9261757.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            7532..7990
                     /gene="mgsA"
                     /locus_tag="ACHK49_001072"
     CDS             7532..7990
                     /gene="mgsA"
                     /locus_tag="ACHK49_001072"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO9261758.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(8022..10076)
                     /gene="helD"
                     /locus_tag="ACHK49_001073"
     CDS             complement(8022..10076)
                     /gene="helD"
                     /locus_tag="ACHK49_001073"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO9261759.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            10199..10645
                     /gene="yccF"
                     /locus_tag="ACHK49_001074"
     CDS             10199..10645
                     /gene="yccF"
                     /locus_tag="ACHK49_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO9261760.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            10655..12817
                     /gene="yccS"
                     /locus_tag="ACHK49_001075"
     CDS             10655..12817
                     /gene="yccS"
                     /locus_tag="ACHK49_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO9261761.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(12780..13409)
                     /gene="sxy"
                     /locus_tag="ACHK49_001076"
     CDS             complement(12780..13409)
                     /gene="sxy"
                     /locus_tag="ACHK49_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO9261762.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            13628..14137
                     /gene="sulA"
                     /locus_tag="ACHK49_001077"
     CDS             13628..14137
                     /gene="sulA"
                     /locus_tag="ACHK49_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO9261763.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            14494..15534
                     /gene="ompA"
                     /locus_tag="ACHK49_001078"
     CDS             14494..15534
                     /gene="ompA"
                     /locus_tag="ACHK49_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO9261764.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(15609..16061)
                     /gene="matP"
                     /locus_tag="ACHK49_001079"
     CDS             complement(15609..16061)
                     /gene="matP"
                     /locus_tag="ACHK49_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO9261765.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            16247..18007
                     /gene="ycbZ"
                     /locus_tag="ACHK49_001080"
     CDS             16247..18007
                     /gene="ycbZ"
                     /locus_tag="ACHK49_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9261766.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLSDFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            18076..18594
                     /gene="fabA"
                     /locus_tag="ACHK49_001081"
     CDS             18076..18594
                     /gene="fabA"
                     /locus_tag="ACHK49_001081"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO9261767.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18664..18831)
                     /gene="rmf"
                     /locus_tag="ACHK49_001082"
     CDS             complement(18664..18831)
                     /gene="rmf"
                     /locus_tag="ACHK49_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO9261768.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(19087..19650)
                     /gene="pqiC"
                     /locus_tag="ACHK49_001083"
     CDS             complement(19087..19650)
                     /gene="pqiC"
                     /locus_tag="ACHK49_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO9261769.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASAWSQEAASIAQEIKRLP"
     gene            complement(19647..21287)
                     /gene="pqiB"
                     /locus_tag="ACHK49_001084"
     CDS             complement(19647..21287)
                     /gene="pqiB"
                     /locus_tag="ACHK49_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO9261770.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            complement(21292..22545)
                     /gene="pqiA"
                     /locus_tag="ACHK49_001085"
     CDS             complement(21292..22545)
                     /gene="pqiA"
                     /locus_tag="ACHK49_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO9261771.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            complement(22675..24582)
                     /gene="uup"
                     /locus_tag="ACHK49_001086"
     CDS             complement(22675..24582)
                     /gene="uup"
                     /locus_tag="ACHK49_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9261772.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            complement(24594..26702)
                     /gene="rlmKL"
                     /locus_tag="ACHK49_001087"
     CDS             complement(24594..26702)
                     /gene="rlmKL"
                     /locus_tag="ACHK49_001087"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO9261773.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLGGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            26946..28055
                     /gene="ycbX"
                     /locus_tag="ACHK49_001088"
     CDS             26946..28055
                     /gene="ycbX"
                     /locus_tag="ACHK49_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO9261774.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PGAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            complement(28052..28594)
                     /gene="zapC"
                     /locus_tag="ACHK49_001089"
     CDS             complement(28052..28594)
                     /gene="zapC"
                     /locus_tag="ACHK49_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO9261775.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(28768..29778)
                     /gene="pyrD"
                     /locus_tag="ACHK49_001090"
     CDS             complement(28768..29778)
                     /gene="pyrD"
                     /locus_tag="ACHK49_001090"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO9261776.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(29889..30626)
                     /gene="ycbF"
                     /locus_tag="ACHK49_001091"
     CDS             complement(29889..30626)
                     /gene="ycbF"
                     /locus_tag="ACHK49_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO9261777.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(30592..31107)
                     /gene="ycbV"
                     /locus_tag="ACHK49_001092"
     CDS             complement(30592..31107)
                     /gene="ycbV"
                     /locus_tag="ACHK49_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9261778.1"
                     /translation="MLKRIIWILCLLGLTGSSELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGMDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(31115..31657)
                     /gene="ycbU"
                     /locus_tag="ACHK49_001093"
     CDS             complement(31115..31657)
                     /gene="ycbU"
                     /locus_tag="ACHK49_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9261779.1"
                     /translation="MKKKTIYQYVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(31669..32739)
                     /gene="elfG"
                     /locus_tag="ACHK49_001094"
     CDS             complement(31669..32739)
                     /gene="elfG"
                     /locus_tag="ACHK49_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9261780.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNEGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(32730..35330)
                     /gene="elfC"
                     /locus_tag="ACHK49_001095"
     CDS             complement(32730..35330)
                     /gene="elfC"
                     /locus_tag="ACHK49_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO9261781.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            complement(35355..36056)
                     /gene="elfD"
                     /locus_tag="ACHK49_001096"
     CDS             complement(35355..36056)
                     /gene="elfD"
                     /locus_tag="ACHK49_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9261782.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            complement(36139..36681)
                     /locus_tag="ACHK49_001097"
     CDS             complement(36139..36681)
                     /locus_tag="ACHK49_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9261783.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAAKKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
BASE COUNT         9096 a         9484 c         8772 g         9354 t
ORIGIN      
        1 aaatcagagc gcaactgata ctctctcgtc gccagtacca gaaactgatg cagtggaaac
       61 ggcaattccg gacaacgaaa aaaccgaatg caaagtggaa gtcgaaccat ctgtagagcg
      121 tgaggggccg ttctacttcc tcttcaccga caaggatggc gaaaaatacg gtcgcgcaaa
      181 caaactttct ggtctggata aggcactggc tgccggggct actgaaatca cgaaagaaga
      241 atatcttgcc cgcaaaaacg gaacatacac ggacttaccg caaaatgtgg ataccgctga
      301 agattccgta caaccggagc cggtaaaagt taccgctgac gaagtaaaca aaattatgca
      361 ggcagccaat atcagccagc ctgacgccga taaattgctt gctgcatcac gtggtgaatt
      421 tgttgaaggg attagcgatc cgaatgatcc gaaatgggtg aaggggattg aaacctgcga
      481 ttctgtgaac cagaaccagc aagaatcgga acagaacgac cagaaaacgg aacaaaacag
      541 cccaaatgcg caacaaaacg agccagaaac gaaacaggag ccggaaaaag cctgcaccgc
      601 ctgcggtcag accggcggcg gtagctgccc tgattgtggt gcggtgataa gtgacgcaac
      661 ataccaggaa atattcgatg aagagaatca gcctgaagtt caggaaaatg atccggagga
      721 aatggaaggc actgcgcatc agcacaagga gaacactggc ggcaatcagc atcatgccag
      781 cgatagtgaa actggcgagg cgtcagatcc cttaattaag gcgaacggtc atcataatat
      841 cacatccacc agcagagcgg ggattcatct gatgatcgac cttgaaacca tgggaaaaaa
      901 tcctgatgcc ccgattatct caataggtgc aatatttttc gatccgcaaa ccggagagat
      961 ggggccagaa ttcagcaaaa ctatcgatct ggaaactgct ggcggagtca ttgatcggga
     1021 caccattaag tggtggctga aacagtcacg cgaagcgcaa tccgccattc ttaccgatga
     1081 aatcccgtta gatgatgcac tgctgcaatt acgggaattt atcgacgaaa actccggtga
     1141 attttttgtt caggtctggg gtaacggtgc aactttcgac aacgtgattt tacgccgttc
     1201 atatgaacgg caggggatcc cctgcccgtg gcgttacacc aatgatcgcg atgtaagaac
     1261 gatggttgct ctgggactgg tgatggattt cgacgcaaga acgactattc cattcgaagg
     1321 tgaacgccat aacgctctga acgatgcacg ttaccaggcg aaatacgttt cagccatctg
     1381 gcaaaaactg ctcccgagtc aggctgattt ttgatgttca acccatatcg ccgcccacca
     1441 gctatagtgg cggcggtcat gctgtaaagg cacgtgacca catgtacgaa ttaactctat
     1501 ctccagcaga gattaaagag atcacgaaat acgagcgata cacaaaacag caacaccagt
     1561 taagactgca cggcatccca tttgtaatcg gccctaaaaa cgaacccata gttctccgca
     1621 gggatattcc acacggtctg acaacgatgc caaaaacatc tgaactggtt tctgctgaac
     1681 ccgattttga ggcgctgaac aatgggaaga ccaagaaaaa ataaaaaaga taatgtactg
     1741 ccaccgcggg ttagatcgaa tggttacagt tacgtgtgga aacccgaagg aagtacaaga
     1801 agtatagggc taggaagagt gcggaaaacc agcgtagcta aagtctggca aaattatgaa
     1861 ctggaaaaag caaaactcca caacataatg accgtagcta aattatggca catgtttatg
     1921 gactcccctg catttacaga actggccccc cgaacccaaa aagattatcg acaacatcag
     1981 aaggcgttgc tgatggtatt cggaaaagtg cttgctgata atgtcaaaac tgagcaggta
     2041 agaattttca tggataaacg agggcttgag agcaagaccc aggcaaatca tgaactggca
     2101 agcctgagtc gagtatacgg gtggggatat gagcgtgggt atgtgaagaa taacccatgc
     2161 aaaggagtca gaaaattctc tcttaaagcc cgcactgttt acatcaccga tgaacagtat
     2221 gcggcgatat atgcggaagc aattccacag ttacgcattg caatggagat ttcctatctc
     2281 tgtgcggcaa gactcggtga tgtgcttgag ttgaaatggc aggatattat ggataaaggg
     2341 atctacattg agcaaaacaa aaccggcacc aaacaaatca aggaatggtc accgcgatta
     2401 cgtacagcga tccagttagc ccgaaatgta tcttcctgta catgcgaata tgtgatcaat
     2461 acaaccaaag gcgggaaagt catagctaaa acgctgaata actggtggaa tcaggctaaa
     2521 cgcgcagccg agcaaaaagt tggcgttccg ttcgggtgca attttcacga cataaaagcc
     2581 aaggggatct cagattacga aggcagcagt cgcgacaaac aaattttcag cgggcataaa
     2641 acagaaaatc aggtgttgat ttacgatcgt aaaacaaaaa tcacaccaac actggatttg
     2701 ccgctcgtgg ttagcaagta gacaatccag aaaaatttct ggcaaaaaga cttggtatac
     2761 tagctatgag caacttccta ataccttgat ttttcttggt gttaaatttg attaactttt
     2821 cagcaatttc acgtattgca ctgattttta tttgtttttc ttcggtcttg aaaaccggcg
     2881 acccgaaagg gttccagagt tcgaatctct gcgcttccgc caaataagat aaggggttag
     2941 ctaaatgcta accccttttt cttttgcctg tcgaaattct cagggcgtta tatttgctta
     3001 atgacctgat aatccgctgt taaacctgtt ccagatcaaa tgcgtaaaga tgggtaaaac
     3061 ttctgggtgc ccttacgcat tatcattatg ctgcttaatt aattacatct gtcatagaga
     3121 gtgactcaat ggatcgtatt gttagttctt cacatgaccg tacatcactg cttagcaccc
     3181 ataaggtgct gcgtaatacc tattttctgc tgagcctgac gctggccttt tcggcaatta
     3241 ccgcaactgc cagtacggtg ctgatgctgc catctccggg tctgattctg acgctggtgg
     3301 gtatgtatgg tttgatgttc ctgacctata aaacggcgaa taagccgacc gggattatct
     3361 ccgcattcgc ctttaccggt tttctgggtt atatcctcgg gcctattctg aacacctatc
     3421 tgtctgccgg aatgggtgac gtaatcgcta tggcactggg cggaacggcg ttagtgttct
     3481 tctgctgctc tgcatatgtg ctgaccaccc gcaaagatat gtcgttcctc ggcggtatgc
     3541 tgatggcggg tattgtggtg gtgctgattg gtatggttgc taatatcttc ctgcagctgc
     3601 ctgctctgca tctggcgatc agcgcggtct tcattttgat ctcctctggc gctattttgt
     3661 ttgaaaccag caacatcgtt catggcggtg aaacgaacta tattcgtgcc acggttagcc
     3721 tgtatgtttc gctgtacaac atcttcgtca gcctgctgag cattctgggc ttcgccagcc
     3781 gcgattaatc tcacccgcta acactttcat agcctcgctt tatgcggggc tttgtttttt
     3841 gttacactgc cgccagtctg attaaggtag taataattat gctgatcttc gaaggtaaag
     3901 agatagaaac ggataccgaa ggctatctca aagaaagcag ccagtggagt gagccgctgg
     3961 cagtggtgat tgcagagaac gaagggattt cgctgtcacc ggaacactgg gaagtggtgc
     4021 gttttgtgcg tgatttctat ctggaattca atacttctcc ggcgattcgt atgctggtaa
     4081 aagcgatggc gaataaattt ggcgaagaaa aaggtaacag ccgctattta taccgactgt
     4141 ttccgaaagg tccggctaag caagccacca aaattgctgg cctgccaaaa ccggtaaaat
     4201 gtatttaacg gatacgaaaa tcggttaatt cccccgaggg atgatgcggc tcgctgagca
     4261 cccgttctac acgcgcagaa cgcgggccgc cactctttaa ccacagcatt aatttttcca
     4321 cctgcccttc ttcaccgcag gcaaccactt ccacgctgcc atcgtcaaga tttttggcgt
     4381 acccggttaa acccagtctt ttcgcttcgt actgtgtggt gtagcgaaat cctacgccct
     4441 gaactcgccc gtaaacccag gcaattatgc agactttcga cattgctgct ctccttatcc
     4501 ttaatggacg cgttgcaaat ccatggaaaa ctccggacaa tgacgcccat ttctttgcat
     4561 cgacagaaaa gtaaattatg agtgtacgtt tagtgttagc caaagggcgc gaaaaatcat
     4621 tacttcgtcg ccatccgtgg gtcttttccg gggccgttgc ccgcatggaa ggtaaagcca
     4681 gcctcggtga aaccatcgat attgttgatc atcagggaaa atggttagca cgcggcgctt
     4741 attcaccagc ttcgcaaatc cgggcgcgcg tctggacgtt tgacccgtct gagtctatcg
     4801 acattgcttt tttttcccgc cgtttgcaac aagcacaaaa atggcgtgac tggctggcgc
     4861 aaaaagatgg cctcgacagc tatcgtttaa tcgccggaga atctgatggc ctgccgggta
     4921 ttactatcga tcgtttcggt aattttctgg tgctgcaact gctgagtgct ggggcagaat
     4981 atcagcgcgc ggcattagtt agtgccctgc aaacgctgta cccggaatgt gcgatttacg
     5041 atcgcagcga tgttgcggta cgtaaaaaag aagggatgga gctgacccag ggcctcgtca
     5101 ccggcgagtt gccgcctgcc ctgctgccga ttgaagaaca cggcatgaag ctgctggtgg
     5161 atattcagca cggacacaaa acgggctact acctggacca gcgtgatagc cgcctggcta
     5221 cccgccgcta cgttgaaaat aaacgtgtgc tgaactgttt ctcctatacc ggtggtttcg
     5281 ccgtatcggc actgatgggc ggttgcagcc aggttgtcag cgttgatacc tcccaggaag
     5341 cgctggatat tgcacggcag aacgttgagc tgaacaaact ggatctgagc aaggctgagt
     5401 ttgtccgtga tgatgtcttt aaattgctgc gtacttatcg cgatcgcggt gaaatatttg
     5461 acgttatcgt gatggacccg ccgaagtttg ttgagaataa aagccagttg atgggcgcgt
     5521 gtcgtggcta taaagatatc aacatgctgg cgattcagtt gctgaatgaa ggcggtattc
     5581 tcctgacttt ctcctgttcc ggtctgatga ccagcgattt atttcagaaa atcatcgcgg
     5641 atgccgcaat tgatgccggc cgtgatgtac aatttataga gcagttccgt caggcagccg
     5701 atcatccggt gatcgctacc tatccggaag ggctatatct gaaagggttt gcctgtcgcg
     5761 tcatgtaact tgaaatgtgg aattttaccc ttatatcaaa ggtaaagatt tcccgggagg
     5821 tgactatgat tgccagcaaa ttcggtatcg gccagcaggt ccgccattcc ctgttaggtt
     5881 atctcggagt ggtcgtggat atcgacccgg tttattcgct ctctgaaccg tcgcctgatg
     5941 agctggcggt gaatgacgag cttcgcgccg ctccgtggta tcacgtggtg atggaggacg
     6001 ataacggcct accggttcat acctacctgg ctgaagccca gctaagtagc gaattgcagg
     6061 atgaacatcc tgaacagcca tccatggatg agctggcgca gaccatccgc aaacaactcc
     6121 aggccccgcg tctgcgtaac tgatccctga acgaatcccc ggaaggtttt tgccttccgg
     6181 ggatttttta tttggccagg cccaggcgag ggatctcaat agccgggcag cgatccatca
     6241 ccacattcaa cccggcatcc cgtgccagta ctgccgcttg ctcattaatc acccccagtt
     6301 gcatccagag ggttttcgcg ccaatggcaa tagcttcctg tgccacgccc cacgccgctt
     6361 ctgaattacg gaaaacatcg accatatcga ccttttccgg aacgtctgcc agcgtgccat
     6421 acccctgctg gcccagcagc gttttgccgg caacttttgg cgaaaccgga atgacgtgat
     6481 acccctggtc gagtagatat ttcatcacgc gatagctggg acgatcgggt ttgtcgcttg
     6541 cccccaccag cgcaatggtg tgagtggacg ttaaaatgcc tgcaatatcg gtttctttca
     6601 ttatttcctc cagactgttt aacaaagtgt acgacaaagc cgacatgcca accatgcagc
     6661 taatacgaat gaatgagagc aaaagcggga aatatgtcta tatgttagta agtatgatta
     6721 aagaaaaatt ttgacacatt attattcggg cggtcttttg acaggagcta cctatgaaaa
     6781 ccggcatcgt gaccaccttg attgcattat gtttgccggt atccgttttt gccaccacgc
     6841 tgcggctttc aaccgatgtc gatcttctgg tactcgacgg caagaaggtt tccagctctc
     6901 tgctgcgcgg tgcggacagc attgaactgg ataatggacc gcatcagtta gtgtttcgcg
     6961 ttgagaagac gattcatctt tccaatagtg aagaacggct gtatatctcc ccaccgctgg
     7021 tggtcagttt taatacccag ctcatcaacc aggtcaattt tcgcctgcct cgcctggaga
     7081 atgagcggga agctaaccat tttgatgccg cgccgcgcct tgaattgttg gatggcgatg
     7141 cgacgccgat tccggtaaag ctggatattc tcgccattac ctcaacggca aaaacgattg
     7201 attatgaggt cgaggttgaa cgctataaca aatccgcgaa acgcgcttca ctaccgcaat
     7261 ttgccacgat gatggcagat gacagtacgc tgctttcggg tgtttccgag ctggatgcta
     7321 ttccaccgca gtctcaggtg ctcacagaac aacggctgaa gtattggttt aaactggctg
     7381 acccacaaac gcgaaatact ttcctgcagt gggcggaaaa acaaccatct tcctgagatt
     7441 tttgtgcctg tgcgcaggct ttttcggtct ttatcttgca gcgataagtg cttacagtaa
     7501 tctgtaggaa agttaactac ggatgtacat tatggaactg acgactcgca ctttacctgc
     7561 gcggaaacat attgcgctgg tggcacacga tcactgcaaa caaatgctga tgagctgggt
     7621 ggaacggcat caaccgttac tggaacaaca cgtactgtat gcaacaggca ctaccggtaa
     7681 cttgatttcc cgtgcaaccg gcatgaacgt caacgcgatg ttgagtggcc caatgggggg
     7741 tgaccagcag gttggcgcat tgatctcaga agggaaaatt gatgtattga ttttcttctg
     7801 ggatccacta aatgccgtgc cgcacgatcc tgacgtgaaa gccttgctgc gtctggcgac
     7861 ggtatggaac attccggtcg ccaccaacgt ggcaacggca gacttcatta tccagtcgcc
     7921 gcatttcaac gacgcggtcg atattctgat ccccgattat cagcgttatc tcgcggaccg
     7981 tctgaagtaa tattgcacag gtggcaaacg ccacctgttt cttacggttt tctcgccacc
     8041 ggcacatcca gatttttcag tatttccaca aagggagagg gattctcttt gttaaacagt
     8101 gcccataccc gatggcgtgc ccgggtcagc gccacgtaca ttaaccgccg ttcttcagcg
     8161 tccgggaaat cctcaaccgg tggcagtagc gcctcttcca taatcgactc ccgcgccgca
     8221 gccggaaaac catcactccc ctcctgcaag ccaacgatga tgacgtaatc cgcctgttgc
     8281 cctttgctgg catgaatggt cataaagtcg atttgcaact tcggccagcg tgttgccgct
     8341 ttttccaggc tggcaggcct catgtgatgg taacgcgcca ggataagaat gcgctcttcc
     8401 ggtttggcat aaccagagag cttatccagc aaagcgtcaa gttgactctc atccaataac
     8461 gtgacggctt tttcgtctcc attggttaag ctgtttagcg gctttttcaa ctggcctggg
     8521 ttctgctgaa taaaccggtt tgccacctca ccgatacgac tgttaaaacg gtaagtcgtg
     8581 tctaaatcac agcgatcgcc ttcaccaaag ttttcatgga aagcggtggt gagcgacatt
     8641 tgcgcgccgc tgaatcggta aatcgcctgc cagtcatcac caacggcgaa caacgtcgtc
     8701 tgactgtttt gcttgcgtaa tgccgctaac aacgctgccc gctgcggcga gatatcctga
     8761 aattcatcaa ccagaatatg cttccacggg ctgataaagc gacctttctc cagaatcaca
     8821 atcgcctgat gaataaggcc cgaaaaatcg acagcgtttt ctgccttcag cgcacctttc
     8881 caggctttta ataacggggc catcaacttg atacgtttac tgaacagatc gcgaatctct
     8941 tcgggtgcac tggcaatcat ttctgcctgt gcaccaccgt gcatccgcat cagacttacc
     9001 cagcgatcga ggcgagaggc aaggcgacgc tgtaattttt catcatccca gaagttacct
     9061 tctggcactg accactgcat ttcttccgtc agccattgcc gccagccctt cgcctgcgct
     9121 ttcttttcgc tgcattgctt gcgccactca gcaataaaga gttcatgacg ggcagcggta
     9181 tcattttcca gtttgctgac tatcggaact tttttgctgc cctgctgaat aatatgcagc
     9241 gccagcgcat gaaacgtgcg tgcggtaatg tcttcggtat gtagccgttc gcgaatccgc
     9301 tcgtccatct cttcagcggc tttgcgacca aacgccagca ataaaatttg ctcaggggac
     9361 gcttcaccac gcgccagcaa ccagcctgca cgggccacca gcaccgacgt ttttccgctt
     9421 cctgcacctg ccagcactaa cagagaatgc tcgccattaa cgactgcccg ggcctgcgcc
     9481 ggattcagcg gtgaagactc gacctggcgg aaaaaatccg catactcggt aagcatggct
     9541 tcggtatacg cctggttatg ctgcaaccga gcgctttcaa tatctttcaa ccaggcctga
     9601 catttacgcc acgcctcacg gcagttatcg aattcttcca gtcggttaac cggcaacggc
     9661 aacgccgaca aagcctggcg gatttgttgc tgcacgccag aggtttgctc acgcgtcagc
     9721 catttatttt ccccagtgcg cgtggcaatc aaatccagtt gctggcgtaa aacaccagac
     9781 gcaatttcgc tcatctcgcc actccaccgc cgccagtgag catcaagatg atggtaaaaa
     9841 cgctgcgtct cgccccattc ggtgccgtgc agacgcacca ctttttcgtc cggcagtaca
     9901 aattccagct cgccccatac cagaccgcgc ttacagtgaa tcgccagtaa ttgattgaaa
     9961 ggaataaggt attcgtggcg atcgccggag actttaatcc cggcattgag gatcaccgcc
    10021 cgatcgtaag ggtgctgtgc cagacgtttt ccaagcgttg tcgctttcag ttccatgact
    10081 ctgtagatcc acacgaaatt atttcttatc agtgtaaccg tcagagaaaa cgtgctccag
    10141 cccaaaaaaa cgttacaatt gccgcccatt actgaaaacc acagtaaagc gaggttttat
    10201 gcgtaccgtt ttgaacattc tgaactttgt gcttggcgga tttgccacca ctctgggctg
    10261 gctgttggcg actctggtca gtattgtgct gatttttacc ttaccgctga cacgatcctg
    10321 ctgggagatc actaaactgt ctctggtgcc ttatggcaat gaagctattc atgtcgatga
    10381 actgaacccg gctggcaaaa atgtactgct gaatactggc ggtacggtat tgaatatttt
    10441 ctggctgatt ttctttggct ggtggttatg cctgatgcac attgcaacgg gcatcgcaca
    10501 atgtatttca atcattggca ttcctgtcgg cattgcgaac tttaaaattg ccgctattgc
    10561 actatggccg gttggtcgtc gcgtggtatc ggtagaaaca gcgcaagctg cgcgtgaagc
    10621 caatgcacgt cgtcgttttg aataatccac taatatggcc tttatgctaa gtcctttgct
    10681 caaacgctat acctggaaca gcgcctggct gtattacgcg cgtattttta ttgcgctttg
    10741 tggaaccaca gcgtttccgt ggtggctggg tgatgtaaaa ctgacgattc cgctaacgct
    10801 ggggatggtg gcagcggcgc tgaccgatct cgatgaccga ctggcgggac gtttgcgtaa
    10861 cctcatcatt acgctgttct gcttttttat cgcctcggcc tcagtagaat tgctgtttcc
    10921 ctggccctgg ctatttgcga ttggcttaac gctctctacc agcggcttca ttttgctcgg
    10981 cggtctgggt caacgctatg caacaattgc cttcggtgca ttgctgatcg ccatttacac
    11041 tatgttggga acatcactgt atgagcactg gtatcagcag ccgatgtatc tgctggccgg
    11101 tgccgtctgg tacaacgtcc tgacacttat tggtcatctg ctgttcccgg tccgcccgct
    11161 gcaggacaac ctggcgcgtt gctatgaaca actggcgcgt tatcttgagc tcaagtcgcg
    11221 catgtttgat cctgatattg aagatgaaag ccaggcaccg ctgtacgatt tggctctcgc
    11281 caacggtctg ctgatggcga cattgaatca gacgaaactc tcgctgctga cccgcttacg
    11341 tggcgatcgt ggtcaacggg gaacgcgtcg cacgctgcat tattactttg tcgcacagga
    11401 tattcacgag cgtgccagct cttctcatat tcagtatcaa acattgcgtg aacattttcg
    11461 ccacagcgac gtgctgttcc gttttcagcg gctgatgtcg atgcagggcc aggcgtgcca
    11521 gcaactgtca cgctgtattt tgttgcgtca gccttatcaa catgatccgc attttgagcg
    11581 cgcttttacg catattgatg ctgcgctgga gcggatgcgc gataacggcg cacccgccga
    11641 tttactcaaa acactgggat ttttgctgaa caatttacgc gccattgatg cccaactggc
    11701 aacaattgaa tcagaacagg cccaggcact accccataat aatgacgaaa atgagctcgc
    11761 tgatgacagc ccgcacgggt tgagtgatat ctggctgcgt cttagccgtc acttcacgcc
    11821 ggaatccgcc ctcttccgtc atgcggtaag aatgtcgctg gtgttgtgct tcggctacgc
    11881 catcattcaa ataaccggaa tgcatcacgg gtattggatc ttgctgacaa gtttgtttgt
    11941 ctgccagcca aactataacg ccacgcgcca ccgcctgaag ttaaggatta ttggtacgct
    12001 ggtaggtatc gccattggca ttcctgtgct gtggtttgtg ccatcactgg aagggcagct
    12061 ggtgctgctg gttattaccg gcgtgctctt ttttgccttc cgtaacgtgc aatacgctca
    12121 tgcaacgatg ttcatcacac ttttggtgct actgtgtttt aacttactgg gtgaaggttt
    12181 tgaagtagcg ttacctcgcg taatcgatac gctgattggt tgtgccattg cgtgggcggc
    12241 agtgagctac atctggcctg actggcagtt tcgcaatctg ccgcgcatgc tcgaacgcgc
    12301 cacagaggcc aactgtcggt atctcgatgc catactggag caataccatc aggggcgtga
    12361 taaccgtctg gcgtatcgta ttgcccgccg cgatgcacac aaccgtgatg ctgagctggc
    12421 gtcggtggta tcaaatatgt ccagcgagcc gaacgttacc ccgcaaattc gcgaagccgc
    12481 gtttcggttg ctgtgcctta accatacgtt taccagctat atctcagccc tcggtgctca
    12541 ccgggagcag ttaactaatc ctgaaattct ggcgtttctt gatgacgcag tttgctatgt
    12601 tgatgacgcg ttacatcatc aacctgctga tgaagaacgc gtcaatgagg cattagctag
    12661 cctgaaacag cggatgcagc aacttgaacc acgggcagac agcaaagaac ctctggtcgt
    12721 acaacaagtt ggattattga ttgcattact gcctgagatt ggtcgtctgc aacgccagat
    12781 tactcaagtt ccgcaggaaa ctcctgtttc ggcgtaagag agtcagccca ttctgcaagc
    12841 tcctggcggc gtgccaccgg gagcgcagct tcatgaatgc cgataatggc accttcaagc
    12901 ataaacagaa tcttttctgt caccagactg ttttgctgcc gcagtcgcaa ccaacacatt
    12961 tttgccccaa gtatacgtaa cgcccgtaca tccttaatcc ccacctcccc gagaatcgct
    13021 tccagatgaa aagacatatt gggcaaatct ttcagtcttt cccgggtatt gcgcgtgctt
    13081 ttctctttca gcgctgcatc gagagaatat ttcgacagac gcaccagctt cagttgattt
    13141 cgccatagac tttcatcaac ccgatagtaa ttgagggtaa cggatcggcc acacttttta
    13201 tatgtcagcc agacaggcgg atgttttaca cagtactgtg cactttgctc acaagcccga
    13261 agatacaact caccatcaga aaccatcgca aacaccgtgt cgtcaacggt caggctgtaa
    13321 ctgccaaaca atgatcggta ttcaattgtg cccaacgttg ccaggtattc ttgtgattta
    13381 tagatccgct tataggagag gcttttcata aaattccttt taaaatcata acataaaaga
    13441 atgattcaca ttaacggatc cgttaactac gaaaataggc aacttattct taaggggcaa
    13501 gattaattta tgttttcccg tcaccaacga caaaatttgc gaggctcttt ccgaaaatag
    13561 ggttgatctt tgttgtcact ggatgtactg tacatccata cagtaactca caggggctgg
    13621 attgattatg tacacttcag gctatgcaca tcgttcttcg tcgttctcat ccgcagcaag
    13681 taaaattgcg cgtgtctcta cggaaaacac tacagccggg cttatcagtg aagttgtcta
    13741 tcgcgaagat cagcccatga tgacgcaact tctactgttg ccattgttac agcaactcgg
    13801 tcagcaatcg cgctggcaac tctggttaac accgcaacaa aaactgagtc gggaatgggt
    13861 tcaggcatct gggctaccct taacgaaagt aatgcagatt agccagctct ccccttgcca
    13921 cactgtggag tcaatggttc gcgctttacg cacgggcaat tacagtgtgg tgatcggttg
    13981 gttggcagat gatttgactg aagaagagca tgctgaactt gttgatgcgg caaatgaagg
    14041 taacgctatg gggtttatta tgcgtccggt aagcgcatcc tctcacgcca cgagacaact
    14101 ttccgggcta aaaattcact ctaatttgta tcattaagta aatttaggat taatcctgga
    14161 actttttttg tcgcccagcc aatgctttca gtcgtgacta attttccttg cggaggcttg
    14221 tctgaagcgg tttccgcgat tttcttctgt aaattgtcgc tgacaaaaaa gattaaacat
    14281 accttataca agactttttt ttcatatgcc tgacggagtt cacacttgta agttttcaac
    14341 tacgttgtag actttacatc gccaggggtg ctcggcataa gccgaagata tcggtagagt
    14401 taatattgag cagatccccc ggtgaaggat ttaaccgtgt tatctcgttg gagatattca
    14461 tggcgtattt tggatgataa cgaggcgcaa aaaatgaaaa agacagctat cgcgattgca
    14521 gtggcactgg ctggtttcgc taccgtagcg caggccgctc cgaaagataa cacctggtac
    14581 actggtgcta aactgggctg gtcccagtac catgacactg gtttcatcaa caacaatggc
    14641 ccgacccatg aaaaccaact gggcgctggt gcttttggtg gttaccaggt taacccgtat
    14701 gttggctttg aaatgggtta cgactggtta ggtcgtatgc cgtacaaagg cagcgttgaa
    14761 aacggtgcat acaaagctca gggcgttcaa ctgaccgcta aactgggtta cccaatcact
    14821 gacgacctgg acatctacac tcgtctgggt ggtatggtat ggcgtgcaga cactaaatcc
    14881 aacgtttatg gtaaaaacca cgacaccggc gtttctccgg tcttcgctgg cggtgttgag
    14941 tacgcgatca ctcctgaaat cgctacccgt ctggaatacc agtggaccaa caacatcggt
    15001 gacgcacaca ccatcggcac tcgtccggac aacggcatgc tgagcctggg tgtttcctac
    15061 cgtttcggtc agggcgaagc agctccagta gttgctccgg ctccagctcc ggcaccggaa
    15121 gtacagacca agcacttcac tctgaagtct gacgttctgt tcaacttcaa caaagcaacc
    15181 ctgaaaccgg aaggtcaggc tgctctggat cagctgtaca gccagctgag caacctggat
    15241 ccgaaagacg gttccgtagt tgttctgggt tacaccgacc gcatcggttc tgacgcttac
    15301 aaccagggtc tgtccgagcg ccgtgctcag tctgttgttg attacctgat ctccaaaggt
    15361 atcccggcag acaagatctc cgcacgtggt atgggcgaat ccaacccggt tactggcaac
    15421 acctgtgaca acgtgaaaca gcgtgctgca ctgatcgact gcctggctcc ggatcgtcgc
    15481 gtagagatcg aagttaaagg tatcaaagac gttgtaactc agccgcaagc ttaagttctc
    15541 gtctggtaga aaaaccccgc tgctgcgggg tttttttgcc tttagtaaat tgaactgact
    15601 ttcgtcagtt attccttacc cagcaatgcc tgcagatcct gcttcagaga agacatttta
    15661 ttcgcgtatt tctctttgtt ttccgcatct tcaatcagct gaacaatcgt ttcagaaagc
    15721 gttttaccgc gacgctgcgc aagaccagcc agacgttgcc agacaataaa ttccagatcg
    15781 atcgattttt tgcgcgtatg ctggtgttcc gcattaaaat ggcgctttcg tcttgcccga
    15841 atggtttgct tcatgcgatt gaccagttcc ggattcatat gcttgtcaat ccagccattt
    15901 accagcacgg gttcattttc gagcgagagc aacacatcga cggcttcctg ggcggcactg
    15961 gcttctatgt aacgggtgat taactcccct tcgcgatgct tcttcaccag atacttccat
    16021 ttccaaccgc tttcaagatt ttcaagttgt tgatatttca tagcgatctc aatgttaccg
    16081 tgtaactctt tacagaatat cagcttttta ggccagtgaa gaaaagaatc tccatcctgt
    16141 gagctttaac gcccatgcca ggagtattgt cggtgctttc tacgtgtgct gtagtgccgg
    16201 ttacggtata atcgcggctt tgacaacaga ctaaaaaaca tcaactttga ccattacgaa
    16261 acttgcatgg cgtgacctgg ttcctgatac cgatagctat caggaaatat ttgctcagcc
    16321 acatttgatt gacgaaaacg atcctttatt cagtgatact caaccgcgac tgcaattcgc
    16381 actggagcag ttgctgcata cgcgagcatc ctcctctttt atgctggcga aggccccgga
    16441 agagtctgag tatctgaatc ttattgccaa tgccgcgcgt acgctacaaa gcgatgcagg
    16501 ccaactggtg ggcggtcact atgaggtttc cggccactcc atccgcttac gtcacgcagt
    16561 gagtgcagat gataattttg cgactttaac gcaagttgtc gctgccgact gggtagaagc
    16621 ggagcaactc tttggctgcc tgcgccagtt taatggcgac attaccctgc agcctggtct
    16681 ggtgcatcag gcaaatggcg gtattctcat catctctttg cgtacactgc tggcgcaacc
    16741 tctgctgtgg atgcggctga aaaatatcgt taaccgcgag cgttttgact gggttgcgtt
    16801 tgatgagtcg cgccctctcc ccgtctctgt gccttcgatg ccattgaagc tgaaagtcat
    16861 tctggtaggc gaacgcgaat cattgtctga tttccaggag atggagccag agctttcaga
    16921 gcaggctatt tatagcgaat ttgaagatac tctgcagatt gtcgatgcag agtcagtaac
    16981 ccagtggtgt cgctgggtga catttaccgc cagacataat cacttacctg caccgggagc
    17041 ggatgcctgg ccggtactta tccgcgaagc agcacgctac accggtgaac aagaaacact
    17101 tccgcttagc ccgcagtgga tcctccgcca gtgtaaagag gtcgcctccc tgtgtgatgg
    17161 cgacaccttc tccggcgagc agctaaactt aatgctgcag cagcgtgaat ggcgcgaagg
    17221 tttcctcgct gaacgtatgc aggatgagat ccttcaggag caaatcctga ttgaaaccga
    17281 aggcgaacgc atcgggcaaa ttaacgccct ttcggtcatt gaatttccgg gtcatccacg
    17341 cgcttttggc gaaccttctc gcattagctg cgttgtgcat attggcgatg gtgaattcac
    17401 cgacatcgaa cgcaaagcgg agcttggcgg caatatccat gcgaaaggga tgatgatcat
    17461 gcaagcgttc ctgatgtcgg aactacagct tgagcaacag atccccttct cagcatcgct
    17521 gacatttgag cagtcataca gtgaagttga tggagatagt gcctcgatgg ctgaactctg
    17581 cgccctgata agcgccctcg ccgatgtgcc ggtgaatcag agtatcgcta tcacaggttc
    17641 agtcgatcag ttcggtcgcg cccagccggt cggtggttta aatgagaaaa tcgaaggctt
    17701 ctttgctatt tgccagcaac gtgagttaac cgggaaacaa ggtgtcatta tccccacagc
    17761 taacgttcgc catttaagtc ttcacagtga actggtgaaa gcggtagaag aaggcaaatt
    17821 caccatctgg gcagtagacg atgtgactga cgcactgccg ttattattaa atctggtgtg
    17881 ggatggcgaa ggccaaacga cgctgatgca aaccatccag gaacgtatcg cgcaagcatc
    17941 gcaacaggaa ggacgtcacc gttttccatg gccattacgt tggctgaact ggtttattcc
    18001 gaactgatcg gacttgttca gcgtacacgt gttagctatc ctgcgtgctt caataaaata
    18061 aggcttacag agaacatggt agataaacgc gaatcctata caaaagaaga ccttcttgcc
    18121 tctggtcgcg gtgaactgtt tggcgctaaa ggcccgcaat tgccagcacc gaacatgctg
    18181 atgatggacc gtgtggtcaa aatgaccgaa acgggtggta acttcgacaa agggtatgtt
    18241 gaagcagaac tggatatcaa tccggatctg tggttcttcg gatgccactt tattggcgat
    18301 ccggttatgc cgggatgcct gggcctggac gcaatgtggc agctggtagg gttctacctc
    18361 ggctggctgg gcggcgaagg taaaggccgc gcgctgggcg ttggcgaagt gaaattcact
    18421 ggtcaggtac tgccgacagc gaaaaaagtg acctaccgta ttcactttaa acgcattgtt
    18481 aaccgtcgtc tgattatggg cctggcggat ggcgaagtgc tggttgatgg tcgtctgatc
    18541 tataccgcca gcgacctgaa agtcggtctg ttccaggata cgtctgcctt ctgattataa
    18601 tcagacagag tatcaaaagg cgaaacctcc gcaatgcgga ggtttctttt taaagagaca
    18661 gaatcaggcc attactaccc tgtccgccat ggcttctcgc cagcctccca gccattgtga
    18721 cctttgattc agcgtctgat agggacacat ttcttttgag cgtccggcga tgccggcctg
    18781 ataaccacgt tgatgtgccc gttccaggcg atctcgtttt tgtctcttca tgcctcgttt
    18841 ccctcatact ggtttctggt ggaaaagaaa acagtgatta ctaaatgtgc aatcacagtt
    18901 aagcaatacc gcgaatcata actgccgtca atgcgcaaaa ttcacgccac tgtcatattt
    18961 gtgagctaca cgggtatgcc ggttgtacaa aaaatgtgaa gaggaacaac tttttccgca
    19021 cgttagagac aaaaaaaccg cctcaggcaa cataccggag gcggttatat ttacaaatct
    19081 ttataattaa ggtagacgct ttatctcttg cgcaatagaa gcggcttcct gactccaggc
    19141 actggccagt actttaacca tctcatcgta accatcctga gtttgcaccc cttccagacg
    19201 gaacggacgt ttgatcagtt gtccctggtg gttcaacagc cactcaccac tgacaatgac
    19261 cttgccatca tagcgaccgt taaactccgt gacggtaaca ttgagcgtgt cctgggcgct
    19321 tcccagaggc tgggaggcaa ccacccagcc gggcagttgc gtactcaggt tggcaaccag
    19381 ggtgttgcgc aactgttgat ccaacgggct ggcccacaag ttgttattgg caatcacata
    19441 cttcacatca ctggtttgat aaaccacacc attccccgcc agatagtcag gaacagtgac
    19501 ctgctctacc cataacagac gattgccctg gctggcggta ctttgtgtac cgctctgcac
    19561 cacaggtaac tggtaatagt ttttattaat ttcgccggag ctgcatccgg ccagccacag
    19621 tgctgcaatc gtcactagcc actttttcat tgtttcgccc tcttcggctc tggatctttt
    19681 ttgtccttcg cttcaaatac cagcgcgtta ctcttctcat tgagcgtttt cagcaccggt
    19741 tgcagttctc gcaacacctg atcaaggcgc tgcatatccg ccaccatctt gttgtaggct
    19801 gcggagccag gctggaagcc ctgcatgctg cgattcaatt cacgcaacgt tgactgcata
    19861 tccgtcggca actgctgcat cgactggcta gcgaggatct tgttcatgct atccagcgtc
    19921 gtttgcaggt ttttcattgt gcgctgactt tcagaaagcg tactggttgc ctgttcaatc
    19981 atcggattca atggcagttt gttgatctta tccaacgctt ccatcagtcg ttgctggatt
    20041 tgcgccaggc cgccgctaac ggtcgggata atctgataac cattaaattc acgaataccg
    20101 gttattgcag gcgtatttgg atagaaatcg agatcaacat acagtgcacc agtgaccagg
    20161 tttccggttt tcagcgatcc gcgtaaacca cgtttcaaca attcgccaag gtgctcaaca
    20221 acatccgcat tttcgccaag ctgcattttc agccgctctg gctcgatacg aatcagtacc
    20281 ggaatacggt aatcatcgtt aaatgtctga cgcatattcg gcgcaaagaa tggcactttg
    20341 cttacggtac ccaggcgaat accccggaac tctaccggag ctcccggttg cagaccgcgt
    20401 accgaatctt taaagaacat cagataatca atgtgatcgg tgtacaacga atcctgaatg
    20461 ctcttctgat catcatacaa aacgaaagct gttttcggtg ccactggctg ccctaaatcc
    20521 agaccttccg gcacatcaaa gctgacaccg ccactcagca gcgttgtcaa tgagcccatc
    20581 tccacacgca tccctgctga cgtcagatca accgcaatgc cactatcttt ccagaagcga
    20641 acattgttgg tcaccagtcg gtcataaggt gcattgatga acagttgata gctgatattg
    20701 cgtttttgtg tatcgaaggt gctggtttca accgaaccta cccgatagcc acggaacagc
    20761 accggatctc ctggcgagag ctgcccggct tttttgctat cgagaatcac acggatacct
    20821 ttcgcatcag gcggggccaa cggtggcgag tccagcaaat cgtatttatc cattttgctg
    20881 cctttcgcgc ctggctgcag ttcgatataa accccagaca gcagggttcc cagaccgcta
    20941 atcccttctc gaccaatctg cggtttcacc acccaaaaga cggtgtcttt atgcagcaat
    21001 ttttccatac cggaattcag ccgcgctttg atttcaacgt gcgtcaaatc atcagccagt
    21061 gtggcgcttt caaccacgcc gacgtcaacg ctacggcttt taatggtggt tttgccacct
    21121 tcaattcctt ccgcattcgc ggtgatcagg gtcacttccg gtccctgatg gctgtaatga
    21181 taaaaaagaa cccaggcccc aatgagcgcc gtgacgatag gaaatatcca cacgggagac
    21241 cagttcttca ctttctggat tttggcttcc ccattattag attccatact gtcaggactc
    21301 ctcatgctct gattcaggtt gacgatccca cgacaaacgc gggtcaaacg tcatagcaga
    21361 aaacattgtc attatgacga ctaaagcaaa cattaatgca cccattgccg gataaatact
    21421 cattaaacct cccatacgca ccagcgccga gagcaccgcg ataacgaaaa cgtcaatcat
    21481 cgaccagcgg cctacaaact caacaacttc ataaatcaaa tgcattcttt cactgtcgcg
    21541 cttgccatgc cctttggcat cccaacacag ccacgcgatg gcgatcatct ttaacgttgg
    21601 caccataata ctggccagaa agatcaccgc agcgacggga taagagcctt cgctccataa
    21661 cagaatgacc ccagcgagaa tcgtcgacgg catcttcgag cctaataaat ccgtcaccat
    21721 gatgggcaaa atattagccg gaagatacag catgatggac gttacaagca gcgcgagtgt
    21781 ccactgcagg ctgtttctac gccgaacgta ccctttggta ctacaacgcg ggcacacggg
    21841 ttcatcagca ggaaggattg ccgtacaaca ggagcacgaa cgcagcccct gacgtatccc
    21901 cgtgacgcct ggttttagcg gctggcgcag ttctggcatc ggggcgatgt cgtcccataa
    21961 ccagcgacga tcaacgcact gaaaagcgcg cagttgcagg acacaaaata aacaccaggg
    22021 gagaaagctg ctgcctaccc caatgctgcc gtaagccatc agtttaacga aactgaccag
    22081 cacaccagcg aggaaaatct ccgccattcc ccaggttttg agttgaaaaa gcacccgtgc
    22141 cagttgctct tttaaacgga ccggtaattc cgcgcgattc accagtaaca gaatggttat
    22201 cagacaaaac gcgggaacca gttgcacaaa caacaggaaa aaggtgccga ggctggcgta
    22261 gtcctcagaa aaaagcacgc cgggaatttc cagtaatgta atttcactgg taactcctgc
    22321 aacgttcata ttcacaaaag gaaacaagtt ggacagcaac agcatgaaca gtgcagccaa
    22381 cgcataggcg gtgggacgct gccgaggggc atcccacgcc acggttaacg ttgtgccaca
    22441 ccggggacat gccgctttct gaccatgctc aaggcgcggt aacgccacca gcatgtcaca
    22501 ctgcgagcac aggatgtgct tcgcggcatg atgatgttcg cacattggta ctccttaatg
    22561 atgcgctatt ttacagatga ctaacaaagt gttggttttc atgcctgata aacccagttc
    22621 atcaggcaat tttaccgttg tcagctgtct ctgtttaaat cgactatttt gcgatcagcc
    22681 accatttttt aacgcttcaa gatactccca gcgttcaaag gcttgctcca gctcctgctc
    22741 tgcagcagcc atatcggcga gcactttttg tgtctgctca tgtggctgac tgaagaagga
    22801 agcatccgcc acctgcgtct gtagggcttc cagcttcgcc tccagatctt cgagcaattg
    22861 cggtagctgc tccagttcgc gctgcaattt atagcttagt ttgctactgc tgcgttttac
    22921 agtttctgct tttgccgcag cggcttcttc ggtttttttc accgcaggct gtttgagcgc
    22981 cacatactgc tcttgctgac cacgggcatc atgataaccg ccgacataac gaccaatttt
    23041 accgccgcct tcgaagatcc aacattctgt aacggtgtta tcgacgaact gacgatcgtg
    23101 gctaaccagc aataccgtgc cctgatagct gtcgatcagt tcttccagca gttccagcgt
    23161 ttcgacatca agatcgttgg tcggttcgtc aagaatcaat aagttgcttg gtttgaggaa
    23221 caaacgcgcc agcagcaagc ggttccgctc accgccagaa agcgcacgta ccggcgtcat
    23281 cgcccgtttc ggatggaaca gaaagtcctg caaatagccc aatacgtggc gtggcttgcc
    23341 gttaaccatc acctcttgct taccttcggc aaggttatcc atcaccgttt tatcgggatc
    23401 cagttccgcg cggtgctgat cgaaataagc cacttccagt ttggtgccaa cgtgaatacg
    23461 cccgctgtcc gcttgaagct gaccgagcat cagtttcagc agcgtggttt tgccgcaccc
    23521 attcggacca atcagggcaa ttttgtcgcc acgtagaacc tgggccgaaa aatctttcac
    23581 cagttgctta ccgtcaacct ggtagcaaac gtcttccatt tcgaaaacga tcttaccgga
    23641 gcggctggcc tcttccacct gcatctttgc ggtacccatc acttcgcgac gttcaccacg
    23701 ctcgcgacgc attgctttca gggcgcgtac gcggccttca ttacgggtac ggcgtgcttt
    23761 gatcccctgg cgaatccaca cttcttcctg cgccagtttg cgatcgaact cggcattttg
    23821 tagttcttcc acgcgcaggg cttcttcttt ttcctgcagg tactggtcgt aattccccgg
    23881 ataggtcacc agcttgccgc gatcgagatc aacaatgcgc gtcgccatat tgcggataaa
    23941 cgaacggtcg tgggagatga aaataatcgt cccattgaag gttttaagga acccttccag
    24001 ccagtcgatg gtttcgatat ccagatggtt agtcggttcg tccagcagca atacgcgcgg
    24061 attgctcacc agcgcgcgtc ctaatgccgc tttacgcaac cagccgccgg aaagcgacga
    24121 cagcgcaaca ttaggatcta accccagttg cgccagcact tcgttgatgc ggttttccag
    24181 ctgccacaag ttgtggtgat ccagctgttc ctgaaccttc gccagttcgt tgagattttt
    24241 ctcgctcggg tcgttcatca ccaggcgcga aatatcgtga tagcgtttca gatattccgc
    24301 ttgttcttca atgccttcgg caacgaaatc ataaacgcta ccctcaacgt tacgcggcgg
    24361 atcctgttgc agacgagcta caatcaaatc ttgctcgtaa ataatgcgac cgtcatccag
    24421 cccttgttca cggttgagga ttttcattaa cgtcgatttg cctgcgccgt tgcggcctac
    24481 cagacaaaca cgttcattat cttcgatatg cagttctgcg ttatcgagaa gcggcgcgtc
    24541 gctgaacgac agccaggcgc catgcatact gattaatgac attactattc ctttcaggct
    24601 gcggtaatca gccagcagtt gtggatctgg cggttacggg cgaaatcctg ggagagcgtt
    24661 ttttgcgtaa tttcttgtgc tttcagtccc agttttgcca ggccgccgag atccatacgg
    24721 aagccacgtt tgttgttcga gaacatgatc gtcccacctg cacgcagcag acgtttcaaa
    24781 tctttcatca gcgccagatg atcgcgctga acatcaaacg catcttccat tcgttttgag
    24841 ttagagaagg ttggcggatc gataaagatc agatcgaact gttcatttgc ctcacgcagc
    24901 cacgccaggc aatcggcctg aatcaggcga tgcgcacgcc cggtcaggcc attcagacgc
    24961 aggttgcgtt ctgcccactc cagataagta cgcgacatat ccacggtggt ggtgctgcgt
    25021 gcaccgccta atcccgcatg cacggtggcg ctgccggtat aagagaacag gttgaggaaa
    25081 tctttgcctt tgctcatctg gccgagcata cgacgggcga tacggtgatc gagaaacaga
    25141 ccggtatcga ggtaatccgt caggttcacc aacaagtgag cgttatattc ggtaacttca
    25201 agaaactcgc ccttctcgcc cagtttctgg tactgatttt tgcccttctg gcgttcacgg
    25261 gttttcagca ccagtttgtt tggcgcaatc cccagtaccg aaatggttgc agcgataata
    25321 tcgaacagac gctgacgcgc tttgtgagca tcaatagttt ttggcggcgc atactcctgc
    25381 accaccaccc agtcggcata acggtcaacg gcaacgttat attccggtag atcggcgtca
    25441 tacaggcggt aacattcaat tccttcctgg cgagcccact tctcgaattt tttgaggttc
    25501 ttacgcaggc ggttggcgta gtcttccgct accatcgccg gtttgctgtc tggagtgctt
    25561 tcggcaacgt ggtaattttt ctgtacgcag tccagcgggc cgtttttcgc cttgtactgt
    25621 ttgtctgcac gcagctgcaa gcagcttagc agatccggcg aggcactaaa caaagagaga
    25681 ttccagccac caaactggtt tttcatgatc cgacccagca ggctatgcag cgcaatcagc
    25741 gccggttcgc tgtccagacg ttccccgtat ggcgggttgc tcaacactgt gccgtacggc
    25801 cctttcggca gcggattggt cagttgcgcg acatctttca cctcaaaggt gatcagttca
    25861 ccaatccccg caagacgggc gttagtgcgt gcacgttgaa tcacccgtgc gtcgctgtcc
    25921 gaaccgtaaa aatgagagct atactcagcc aggcctttac gggcgcgagt ttgcgcttcc
    25981 gctttcactt cctgccagat agcttcatca tgctgcgccc agccgctaaa gccccaacgc
    26041 ccacggtgca agcctggtgc gcgatcggtc gccagcatcg ccgcttcaat cagcaacgta
    26101 ccggaaccac acatcggatc gagcagcggt gttcctggct gccagccgga tcgcatcaca
    26161 atagcggctg ccagggtttc tttgatcggc gcaataccag cacgatcgcg atagccacgc
    26221 agatgtaaac catcaccact cagatcaaga gcgatactgg cggtttcttt atgcagccag
    26281 acgttaacgc ggatatccgg cgcatcgcga tcaacatttg gacgcggcag atttttccgc
    26341 gtgaaagcat cgacgatcgc gtctttcact ttcatcgcgc cgtactgact gttgcggatg
    26401 gtgtcattca aaccactgaa gtggacagcg aaggtcgcgc caggattaaa catctctgtc
    26461 cagttgatcg cctgaacacc gagatagagg tctaaatcgc tgtaaacctt acactcgccc
    26521 agcggcaaca taatacgcga ggccaggcgg ctccacatca ggctctggta aacaagccgt
    26581 gtgtcgccct tgaaatggac cccaccctga accacctggc attcaacggc ccccaggttt
    26641 tccagttcag tttttaacag ctcttccagc ccacgggccg tactggcaaa cagagaattc
    26701 atatcgtcac ttttactcta agagcctacc gcagtaggcg taattgttgc agtcagtctg
    26761 gacatggaca gcgcggagaa accggagcgt acacaagtac gtgaggattt cgagcacttc
    26821 cctgggccaa actgacaaat aaaatagcct aacgggacag gcgctaagaa aattgttgcg
    26881 cattatagct aataagcgcg tcatgtcata aagttgaggg cttattttca tttgaggacc
    26941 gcaccgtggc gacattaacc cggcttttta ttcatcctgt taaatcgatg cgcggcattg
    27001 gtcttacaca taccctggca gatgtgagtg gtctggcctt cgatcgcatc tttatgatca
    27061 cggaacctga tggtacgttt attactgctc gccagtttcc gcaaatggta cggtttactc
    27121 cttcacctgt gcatgatggc ttgcatctca ccgcaccaga tggcagtagc gcatatgttc
    27181 gttttgctga tttcgccaca caagacgcac caaccgaagt ttggggcaca cattttaccg
    27241 cgcgaattgc gccaggcgcg atcaacaaat ggctaagtgg atttttctcc cgcgaagtgc
    27301 aattacgctg ggtggggcca caaatgaccc ggcgcgtgaa acgccacaac actgtacctc
    27361 tgtcatttgc tgatggctat ccttaccttc ttgctaacga agcctcgtta cgtgatctcc
    27421 aacaacgttg tccggccagt gtaaaaatgg agcaattccg ccccaatctg gtggtttccg
    27481 gcgcgtcagc ctgggaagaa gatcgctgga aagtgattcg cattggtgat gtggtgtttg
    27541 atgtggttaa accttgtagc cgctgtattt tcaccaccgt cagcccagaa aaagggcaaa
    27601 aacatccggc aggcgaacca ttaaaaacat tgcaatcttt ccgcactgcc caggataacg
    27661 gcgatgtcga ttttggtcag aatttaattg cccgtaatag cggcgtgatt cgcgttggcg
    27721 atgaggtgga aattctggca acggctccgg caaaaattta cggcgcagct gccgctgatg
    27781 ataccgccaa catcacgcaa caaccggacg caaatgtaga tattgactgg cagggacagg
    27841 catttcgtgg aaataaccaa caggtgttgc tggagcaatt agaaaatcag ggaattcgta
    27901 tcccttattc ttgccgcgcg ggcatttgtg gaagttgccg tgttcagctt ttagaaggcg
    27961 aagtcacgcc gctaaaaaaa tcagcaatgg gcgatgatgg caccattctt tgctgtagct
    28021 gtgtaccgaa gactgcactt aagttggcgc gttagactgc ctgttcgagg ctgaagctgt
    28081 caacattaac ctgcggtttc agcctgtcgt tcatgatttt aatggcgtcg cccaactgca
    28141 tcgcgcgacc cgcaataaca acgcagggtt gtgccagcag gcatagtgcg gcattttcgc
    28201 cagattcaac gaccagcaaa ttgacctgct cgtgagtatc gctcaaccaa acgcaggccg
    28261 catcgcccgg catgggcacc cacatttcac catgggaaac aaaatgccag cttttcggca
    28321 tttgcggttt gagatagcga attgctacca gtgcattcag caccagttcg gctttttgtt
    28381 ctttagataa attaaaatcg cggcactttt cttcaaaaga gaaatagagc gcggcgtcgt
    28441 caacgcaaaa gcctgcggga gaaaaggcat ctggagtcaa catcttgcgc gcaaaacgtg
    28501 agcgaaatag cataccattg gctaaatcga gcatcattcg atcgtgctct tcatcgtaat
    28561 accaacgcca gttatcgtct ggtttaattc gcatctgcct ctcccctacc ctataaaacc
    28621 gcagcaacac aatccattgt cgcttcgctt aataacctca acaataaaag tacaaaatgt
    28681 ctaaataagc aacagcgatg aatataaaac aaccagggct gaaaataaag ccctgattat
    28741 cttataaaga ggaattcgaa gaaataatta gatatgggta acgatttctt taatcagcgg
    28801 cggaccttta aaaataaaac cagaataaat ttgcaccagt gaggcacccg cagcaatctt
    28861 ttcacgcgca gcgataaccg agtcgatgcc gccaacaccg atgatcggta agcgaccgtt
    28921 taattccagt gacaagcggc gaataatttc ggtgcttttt aactgaagcg gacgaccact
    28981 taagccaccg gtttgatcgc aatttttcat tccctgaaca agagaacgat cgagtgtggt
    29041 attggttgca ataacgccat caatattatg gcgaactaaa ctatcggcaa cctggatcaa
    29101 ttcttcttca gaaagatccg gcgcgatctt cactgcgatc ggcacatatt tatggtgcat
    29161 cgcttgcaaa tcattttgct tatttttaat cgcggttaag agatcatcca gcgcttcacc
    29221 atattgcagc gtgcgtaatc ctggggtatt cggcgatgaa atattgatgg cgatatatcc
    29281 cgcataggca tagatttttt ccatacaaat cagatagtca tctttgccct gctccactgg
    29341 cgtatcttta tttttgccga tgttaatacc caggacgccg tcataatggg ccttttttac
    29401 gttctctacg aggttatcaa cgccaagatt attaaagccc atacggttga tcaaaccttc
    29461 ggcatctacc agacgaaaga gacgcggctt gtcattacct ggctgtggac gtggcgtgac
    29521 ggtaccgatc tcgatcgatc caaatcccat cgcgcctaac gcgtcaatgc actccccgtc
    29581 tttatcaaga ccggctgcca gaccaagcgg atttttaaac gtcaggccca tgcagttaac
    29641 aggtttcgca ggcactttct gccgcaccag tgcttcaaac ggcgttcctg taatacggcg
    29701 taattgctga aaagtaaact catgagcgcg ctctggatcg agctggaaaa gggctttacg
    29761 aacgaagggg tagtacatga actctcctgg attcccggtg tgcaaaccgg gggcgtatta
    29821 tgtgcgagtt cggaccaaaa gggaattgac ctgcggcaaa aaaaacgcaa acgttttccg
    29881 aacctgtttc agggcagatt ttgctccttc tttgcactca acccgccgta atcatttatc
    29941 gcccgccagg cgacatgaca cggtgcgatt gcctgacacc agcttgtctg acgctggctt
    30001 ttaggcggca caactcccgc attacttaac gctttaccat ttacgctcaa ctgaatcagg
    30061 ttaatgtagt aaggcgttgg gttagtgagt tgtacaccct gcgaattccg tgtccagcgt
    30121 aattgttgat aagcttccag cgggtcgccc ggcaaacctt cgggtcgcca gaacagttta
    30181 aataccgagc gcatcgccac ttttacgctc tgattttcaa ctttgccgga tggcacgctg
    30241 gcaatgctta actcaaatag cgtttcgcga tccacaggca agatgtcgct ttccgttctc
    30301 agcaagcgca acgtgcctgt agtaccgggc ttcaggagaa taagtggcgg agcggccagt
    30361 aatggtgctg gcactgtcga cgcttccccc ccggcccaac gcgttgggcg gttgatttta
    30421 ctgttaataa gccaggattc ctgactggta ttagtcagta aaatagatat cgattctctg
    30481 tctgccggaa agataaatcg cgttccgccg accaccactc ctgcccgcaa ttcacccgcc
    30541 accagcagcg ataaaacggc gaaaatcaag agcgccaatc tgttccacgt attagtcata
    30601 atgaagtaca aatgtcgcgc tggcattcac tttacccgac tgcacatcgc tattgacagg
    30661 tcgcaattgc gcataaaaag ttaatggcac cttaccgcta tgtaggctgt agtctttact
    30721 cgcctggtta agcggaatca atatcttcgc atcatccaga atcgcaattg ccagcccgct
    30781 ggctgcatcc ataccacttt caatggacag catttcgctc tgcactccgt cgggggttcc
    30841 tgaaaaggtg acgtcaaccg tgctgacatc tgttccgcaa tcctgcaaat taatgatgaa
    30901 agatttctcc gggccataat tcccgtgaga aaaacgtgtc agcgagacat cgcctaactg
    30961 cacattgatt tgcttgctat cctgtgccag cacgcatgta ttacgacgaa acgatccgct
    31021 aatgttgacc gtgccatcat gagcaaataa ctcactactc cccgttaatc ccagtaagca
    31081 cagaatccag attatccgtt tcaacatcac tccttcactg gtaaaccaga tcaaaataga
    31141 gaaccgccgt cgcattaccg cccgttgctc ccgcctttgt ggacttataa cgaagttgat
    31201 atttgagtgt gttatccccg gcttttaagg gcgtaagagg ctggacctga ttgagcggga
    31261 tttcttgctg actttgcgca tcaagaattt gcacggcaat ccccgttgcc ataccaccgc
    31321 ttccgcctgt gtcagttagt ttaagcagtg tcggcgcaag ggtgtcagcc tcgccgctaa
    31381 aaaggacgtc cgcgccagta ataccagcgg cacaaccggt gatatcgata ttcaaatcag
    31441 ctgcagtgct tacggtattc gcggcctgaa aatcactgat attaaaatcg ccaatatgaa
    31501 tattttgtaa tgcacttttg gtggcgacat cacagccctg atcgaccaca ttcccataaa
    31561 tatgcatact gacttgttca ggcccagcca tcccgacatg gatgttaaga aggctaaaga
    31621 acaaaataac gtactgatat atcgtttttt tcttcatacg ttcacgcgtt aatcaaaatt
    31681 tattctcacc gttgccgtgg cttcaaatcg cccggtttct ggtggtacgc ctgtcgtgct
    31741 gactggatag acattcattc gcgcgatact gtctgcatca tcaggaatag agagcggtag
    31801 tgtcccacca gcaggggaaa taatgttgtt ctggctatcg gtcaccacca cgcccacgcc
    31861 aggacgtgac gtcttaatcg cctggggata gctggggtct gaggttgccg tcagactcag
    31921 gttgacgtta actaaccctt cgtttcccgt gttacacctg actggaattg cgagttctgc
    31981 cgttctggcc ccaaggggtt tatgccctac aacacgaaag ttagccgcaa atacgtcgcc
    32041 cagatcgacc tcgatagtct ctccggcatt gacggaacat gagccaagtg aattgatcgt
    32101 accagacagg taatacacta aaaccggtgt acctgtagtc gtgcaggaat cacctgcggg
    32161 tatgttgtag cattcaaata atcgcgccag cgccatagga ggaataacga ccgaaccaac
    32221 gaacggctgc aatattttca gggaaaagct accgctatta ccgctagccc cctggccacc
    32281 taaacgttga ccgccgggat cgcaaatatg ccatcgtgta tctgcccctg tgcccacgtt
    32341 ttcgaaagga accgcagtag gagaactgcc tttaacctgc aataaaactt tggcggcaag
    32401 atagtcatta attttgtacc agttgtcgcc taaatagact aacggtgaat ccgcggcgaa
    32461 tgcccatcgg ccacttctga cattgtcttt atcgcaatca caggaaacat aataatcatt
    32521 cgattcgttc cactgataaa atttgtcttt aactactcca gccgtatttt cttcttcctc
    32581 cagggtttta acgccaaaat tgacgccaat atacggattc ccctgagtgg tatgacactg
    32641 cccgctataa gcagcagttt gagcgcacca tagcattaag acggcggcaa aaaatagtcg
    32701 tagtaataac acgcattttt ctccaaagat tatctgcata tggcatccat tcgcgtaatc
    32761 gcagagttaa tttgttgttc ggtaagctgg taactgatat gacaaatttg atcgggagct
    32821 tcgccccagc gtacagttat cgctccctga gcgggcaaac cggacatata gagcgagcca
    32881 tcatcatcaa taatgccgct attagtctgt atgccgtcca gcgtcgctat cgcgccaaaa
    32941 cgcagcggta taccctgctt tgatgttttc attaataccc ttgctcccgc atgggcattg
    33001 aactccgcca gaaccaacgc ccctttggtg ggaaccacgt tgactactgc attttcaaga
    33061 tccacgttac gttttaacga tgccgcatca agtgcgattc gattttcccg ataagaagtg
    33121 gcatagggga caacggtata tccgcgccag tcagttttca gcccggtgct gttcgccaca
    33181 cgagtattct ctgcgcctgg tgctgcaatc agcacgttag tttcccccag cgattgcccc
    33241 agggtaatgc cctgtgaatg ggcaactaaa ctgcctgaaa gagcgtagtt cagttgttgt
    33301 tgactgccgt tatcgctgta gttgtagccc actcgggcat ctgcaaacgc ccctttgtag
    33361 tccatgctgg cgctaccatt agccgttttc ccctcgctgt tatatccctg ttgcacgctg
    33421 tagctcaggt taccgtcatc gagtagtgtc tccgttaaac ccaggttttg tgtaatgtgc
    33481 ccttcgttat cgatactggt gttagaggtc atccaggcgt aatttttttt cctggtatag
    33541 ctatcattac ttctggggag caacagattg agcggcaggg aaaaatttag tgcaaacacc
    33601 tgatcagcat caggttgacc acgggactta ctgtagttcc aggaaacccc cagcgagaga
    33661 tctttaattt gcgtgttgta gccaaactgt aatagccgat cctgttgatc ggtatgccag
    33721 taagtttgct ggctaccact taaataaaac gaaccgtact cgcctaattg ctgggagata
    33781 ttgacctgca gtttgccacg tttggtgtaa aacaaattgt aataacgcga ccacatcggc
    33841 gtatcttcat catcaccgtc attaaattcg tagccatcca tatgtttata catggtgtcg
    33901 gaaagggtgt agaaacccga cgtcgaatag cgatagccca tcaattgaaa attagtgccc
    33961 aattggttga gcgttttggc atacagaaaa cgatatgact gacctttatg ttcgctttgg
    34021 tctgccagcg tactcttcgc ctgggtcgca tcgaacgaaa ttgctccgaa atcgccaagg
    34081 ttaaatccca gaccaaacat ggcggcacgg taatattcag catattgtcc accaccatac
    34141 catgtcgttc cccacggtcc gccccactgc aacgtggcct gggcaaattt gctctcctgc
    34201 tgttcattac tattggttct gtatttcgcc agtgtcaccg cgtatttgat tcgcccctga
    34261 cgctggagta atggcacgct ggaaaacggt acgctgtagc tattgacgct gccgtccgct
    34321 tctttgattt caactaacaa atcaccgctc gacgacgtgg aatagagatc actaatttca
    34381 aaagcaccag gcgatacata agtttggtaa atggtgtaac cattttgctt aatcgttatc
    34441 tgggcattgc ttttggcgat accacgcacc acaggcgcaa accctttcag gctgtctggc
    34501 agcatgttgt catctgacgc cagctgcaca ccacgaaagc tgacactatc gaaaaaatcg
    34561 cctgcagtat aatcatcacc tacggtcagt tcgcctttta gtggaataac cgcccgctgc
    34621 aaatagctgc tgagattctt ccattctgcg gtttggccac tactgcgact ccatgttgaa
    34681 ttgttgcgca atctccatgg gcctaaatta acgccactgt ttaaattcag aaaatagctg
    34741 tcgccagaat cactgtctgc gctgctatga atactgttag ccccgctaaa cgaatatccc
    34801 agtaatagcg cgttgatccc ttcatcccaa cgttcagggg ggacataacc acgggcatta
    34861 cttttgatgg cgatttgcgg tactgaaatt tccagacgga gtttattcac attaaacgtt
    34921 acgctggcat ccggaataat ctccgccaat ggcacacacg cttgtttgtc cagttgttta
    34981 aaagccggaa acgcatcagt attaacaccc attgcatcca ggctttcagt cgtcaaacag
    35041 gcggcaatcc ccttctctgg cgttatctca acaaaattga cgttgcgcgt atctacaatt
    35101 gtctgattca gaacaatatc gacacgataa acccctggcg ttattttttg tcctttttcg
    35161 aaacgagaca aatcggccac cattgaggga tcatctttga aaaaagcagg atcgaaccag
    35221 gtttcagctt gtgcattgaa cgctgccgac acaaacacca ccagcccacc aataaacgat
    35281 ggcactccac cagagcttaa caggctgtgt tgtcgatgag ttctatacat ctcgctctct
    35341 taactgaaga cggcttacga tttacagttg agttgcggcg tttccgcccc gtagtcattg
    35401 atggttcgat acgttacgct gctatttttg gccccctttg gcagagaaaa ttggtattta
    35461 tcaaaggggg aaatcatttg atttttaact acttttccac ctattttcag gtctgtcagg
    35521 gtaatgaaat aaggtgttgg gttactgacc gtaaccatgt cagctgacga agtgcaacgc
    35581 agtttcgccg gagcctcgcc taagggatac gctaaacctt ttggtcgcca gaaaagtttg
    35641 atgacgcttt gtgtggcaat cttcagggta tttccctctt cccgcttcgt tgttgaaggg
    35701 actgcccgta cgctagtgaa gaaaagggtt tctctgtctt tcgccaacgg cgcaccaatg
    35761 tacataatgc gtaacagatt ctcattagcc ggattcagca caaatagcgg tggtgtaata
    35821 ataaaatctg ctgatttttt attatctggg gtcatcaccc atgactgaat aagataagga
    35881 acatccggac tggtgttacg tacacccagt gaaatttgct cttcaccttc tgaataaaca
    35941 agacgtgttg ctgcaagtcc aatcccgcct ttacctgccg cccatgcgga tgagcagctg
    36001 agtaatattg ccgttaaact tacggtcaca attccctttg ttatgcaagt tttcataaat
    36061 agtcactctt aaaaaatatg aatatcccct ccagaacact agaggggtac taattaataa
    36121 aacaattaat gagttctatt aataatagtt aatatggaaa tttgttaccg cattcacatt
    36181 accagaagtc gctttccccg tagcgatata atcaacttta aaggtcactg tttgctcagc
    36241 agaatccgct aactgaatat tcgacgtttc ggtaccggga gttaccgatg tagaaccatc
    36301 tggcagatac atttgtagag caacattcgt cgctgcatcg gtggtagcct ggttggcaaa
    36361 tgctgtaggt tgcacgccgt cagccgtacc actaaaggta aacgtcgcat ttttagttac
    36421 agtaacatca cagccttcta aggcaattgg tacatcagtt ttaacgccag agactgctcc
    36481 attagtttgc aacttggtgg cgctgacatt tggcaatgtg actactgaat ctttcttcgc
    36541 agctaccagg gtacaagctg gagcaataac tttaccatta aatgttactt tgccatccgt
    36601 gatagcatta tcatcagctg ccataacgct ggacgttgca cataccacag ttataaacgc
    36661 cgtcaataca ctttttttca tcgtcatcat cctgatctct tcgtaa
//
LOCUS       DAZGLJ010000024        35295 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000024 DAZGLJ010000000
VERSION     DAZGLJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.024"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..473)
                     /locus_tag="ACHK49_001098"
     CDS             complement(<1..473)
                     /locus_tag="ACHK49_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO9261784.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVGIEYIFVAEKIVHYAESNLEKKLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLEEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALH"
     gene            complement(733..1986)
                     /gene="amiC"
                     /locus_tag="ACHK49_001099"
     CDS             complement(733..1986)
                     /gene="amiC"
                     /locus_tag="ACHK49_001099"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO9261785.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2218..3549
                     /gene="argA"
                     /locus_tag="ACHK49_001100"
     CDS             2218..3549
                     /gene="argA"
                     /locus_tag="ACHK49_001100"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO9261786.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3611..5437)
                     /gene="recD"
                     /locus_tag="ACHK49_001101"
     CDS             complement(3611..5437)
                     /gene="recD"
                     /locus_tag="ACHK49_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO9261787.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMVL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVVSGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(5437..8979)
                     /gene="recB"
                     /locus_tag="ACHK49_001102"
     CDS             complement(5437..8979)
                     /gene="recB"
                     /locus_tag="ACHK49_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO9261788.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVELAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEELELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8972..11860)
                     /gene="ptrA"
                     /locus_tag="ACHK49_001103"
     CDS             complement(8972..11860)
                     /gene="ptrA"
                     /locus_tag="ACHK49_001103"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO9261789.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSDKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQHLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEDQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12036..15404)
                     /gene="recC"
                     /locus_tag="ACHK49_001104"
     CDS             complement(12036..15404)
                     /gene="recC"
                     /locus_tag="ACHK49_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO9261790.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLADDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSVEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPDDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15417..15740)
                     /locus_tag="ACHK49_001105"
     CDS             complement(15417..15740)
                     /locus_tag="ACHK49_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO9261791.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15725..16132)
                     /locus_tag="ACHK49_001106"
     CDS             complement(15725..16132)
                     /locus_tag="ACHK49_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO9261792.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(16129..16692)
                     /locus_tag="ACHK49_001107"
     CDS             complement(16129..16692)
                     /locus_tag="ACHK49_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO9261793.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16683..17153)
                     /locus_tag="ACHK49_001108"
     CDS             complement(16683..17153)
                     /locus_tag="ACHK49_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO9261794.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17337..18131)
                     /gene="thyA"
                     /locus_tag="ACHK49_001109"
     CDS             complement(17337..18131)
                     /gene="thyA"
                     /locus_tag="ACHK49_001109"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO9261795.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18138..19013)
                     /gene="lgt"
                     /locus_tag="ACHK49_001110"
     CDS             complement(18138..19013)
                     /gene="lgt"
                     /locus_tag="ACHK49_001110"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO9261796.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19164..21410)
                     /gene="ptsP"
                     /locus_tag="ACHK49_001111"
     CDS             complement(19164..21410)
                     /gene="ptsP"
                     /locus_tag="ACHK49_001111"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO9261797.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21423..21953)
                     /gene="rppH"
                     /locus_tag="ACHK49_001112"
     CDS             complement(21423..21953)
                     /gene="rppH"
                     /locus_tag="ACHK49_001112"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO9261798.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(22306..22452)
                     /gene="ygdT"
                     /locus_tag="ACHK49_001113"
     CDS             complement(22306..22452)
                     /gene="ygdT"
                     /locus_tag="ACHK49_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO9261799.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            22638..23327
                     /gene="mutH"
                     /locus_tag="ACHK49_001114"
     CDS             22638..23327
                     /gene="mutH"
                     /locus_tag="ACHK49_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO9261800.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23396..24109
                     /locus_tag="ACHK49_001115"
     CDS             23396..24109
                     /locus_tag="ACHK49_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO9261801.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            24247..24465
                     /gene="ygdR"
                     /locus_tag="ACHK49_001116"
     CDS             24247..24465
                     /gene="ygdR"
                     /locus_tag="ACHK49_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO9261802.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24573..25613
                     /locus_tag="ACHK49_001117"
     CDS             24573..25613
                     /locus_tag="ACHK49_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO9261803.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25645..26838)
                     /gene="lplT"
                     /locus_tag="ACHK49_001118"
     CDS             complement(25645..26838)
                     /gene="lplT"
                     /locus_tag="ACHK49_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO9261804.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26831..28990)
                     /gene="aas"
                     /locus_tag="ACHK49_001119"
     CDS             complement(26831..28990)
                     /gene="aas"
                     /locus_tag="ACHK49_001119"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO9261805.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            29576..30607
                     /gene="galR"
                     /locus_tag="ACHK49_001120"
     CDS             29576..30607
                     /gene="galR"
                     /locus_tag="ACHK49_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO9261806.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(30614..31876)
                     /gene="lysA"
                     /locus_tag="ACHK49_001121"
     CDS             complement(30614..31876)
                     /gene="lysA"
                     /locus_tag="ACHK49_001121"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO9261807.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31998..32933
                     /gene="lysR"
                     /locus_tag="ACHK49_001122"
     CDS             31998..32933
                     /gene="lysR"
                     /locus_tag="ACHK49_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO9261808.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32920..33612)
                     /gene="ygeA"
                     /locus_tag="ACHK49_001123"
     CDS             complement(32920..33612)
                     /gene="ygeA"
                     /locus_tag="ACHK49_001123"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO9261809.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33741..35159)
                     /gene="araE"
                     /locus_tag="ACHK49_001124"
     CDS             complement(33741..35159)
                     /gene="araE"
                     /locus_tag="ACHK49_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO9261810.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8685 a         9656 c         8677 g         8277 t
ORIGIN      
        1 atatgcaatg ctataaaacc aatctcatca tccggtagtt gaacgctaaa ttgctcatta
       61 atgattgtta atgcatcacg acttattgca tactctgctt tgtacaacgc tttgatttcc
      121 tcaaggaaaa cattattaat ttgtatgcct gagacgactc gggatattgc attcgaaatg
      181 tgatcagcaa gaatcagtag tagcgatgga ttgagttttt tttcgagatt actctccgca
      241 taatgcacta tcttttcggc aacaaaaata tactcaatac ccacatgctc aatcaatttg
      301 tagagtttat ttttttgctc atttctgaca taaaaaatcc ggtttgcagg atgctcaggg
      361 accgacatac catatttttt attgtaacca acgccaggcc cagaaatgat aacctcctgt
      421 ccgttcaccg atgcctgtac acaattattg ttcatgactt tttcgatgat cattccatgg
      481 ctcccaaaaa aaaaggcaag gcatgtcaga agtaataatt acttcaaaca ttgccttgcc
      541 tgatttaaca gttacaagat aattaactaa tgcgtatacc ttacacggaa ttgtgcttaa
      601 taaaagagat gacaggctta ttttttaagc cgatattcaa atattcgaca gcagtcacaa
      661 aaccatcgtt caacatttat gtaaataata tttattcaat gaaacataaa ttaattgaat
      721 aaattaaaaa catcatcccc ttctcgccag cgtcgcccca tcggcaaaat acgctttaat
      781 ccccgcaaga atagactccg caacttcctg ctgaaaggtc gccgttttca gtttacgctc
      841 ttcctcaacg ttactgataa acgccgtttc gaccagaatg gagggaatat ctggtgcctt
      901 tagtacggca aacccggcct gttcaacctg atttttatgc agcttgttga ttttaccgag
      961 cttattcagt accgctttac caaacttaag gctgtcagca atggtcagcg actgcaccat
     1021 atcgaacatg gtgtggtcga catagcggtc accgcttttg ctcacgccac caatcaagtc
     1081 cgaggcgttc tgggtttgtg ccagatattt tgccgcagta ctggttgcac ctttggttga
     1141 gagcgcaaac acagaggaac cgctcggctg acgactggta aaggcgtcgg catggataga
     1201 gacaaacagg tcagcacgct gtttctgggc ttttgctacg cgcacttgca acggaatgaa
     1261 gatgtcttca ttgcgcgtca tgtacacctt catattgccc tctttctcga tcagagagcg
     1321 caaacggcga gctatttgca ataccacatc tttttctcgc gttttgtatt tccccaccgc
     1381 accggagtct tcgccaccgt ggccagggtc aagcataatg acaatcggac gatcgcgccc
     1441 tgctttaccc ggttgtggac cactttgtgc tggcggcacc tgcttttcga ggtcgccttt
     1501 gttgtaatcc tccagcagcg ccagcagcgg gtcctgcata tcctgtgcat tggccggata
     1561 gaggtccatc accagacgct ctttaaaccc ggcgaccggc gcaagggcaa acagctgcgg
     1621 ttttacgttt tgctttaatt caaaaaccat ccgtacggtc tgcgggtcaa attgcccgac
     1681 gcgcgccgac ttgatgaacg ggtcgtctgc acgaatttgt gccgccatcc ccttgagtac
     1741 cgagttcagg tttacatctt cgatatccac caccacgcgt tccgggttac tcaacgcgaa
     1801 ctgcttatat ttcagctgac gatttgattc taccgtcaca cgggtgtagc tggacgcagg
     1861 ccagacgcgc accgccacga cctggctgac cgcagccaga ctgacctgac ttacgctcaa
     1921 tagccacatg gcacccgcgc cttgcagtaa acgacgacgg ctgattgcag tgttggatcc
     1981 tgacatgcct ctcccgagca aaagaaatct aagctgtgta acaagtaaac gactaatttg
     2041 accggaaact ttagcgaaag acgcataatc tgtcatctaa taaacggtaa acattctttt
     2101 tatattcacg gcattactga taaaaaagtc gctctcgcat aaaatttaca cttgcaccct
     2161 gcgaaaaaac agaataaaaa tacactaatt tcgaataatc atgcaaaggg gtgtgccgtg
     2221 gtaaaggaac gtaaaaccga gctggtcgag ggattccgcc attcggttcc ctatatcaat
     2281 acccaccggg gaaaaacgtt tgtcatcatg ctcggcggtg aagccattga gcatgagaat
     2341 ttctccagta tcgttaatga tatcgggttg ttgcacagcc tcggcatccg tctggtggtg
     2401 gtctatggcg cacgtccgca gatcgacgca aatctggccg cgcatcacca cgaaccgctg
     2461 tatcacaaga atatacgtgt gaccgacgcc aaaacactgg aactggtgaa gcaggctgcg
     2521 ggaacattgc aactggacat cactgctcgc ctgtcgatga gtctcaataa cacgccgcta
     2581 cagggcgcgc atatcaacgt cgtcagtggc aattttatta ttgcccagcc gctgggcgtc
     2641 gatgacggcg tggattactg ccatagcggg cgtatccggc ggattgatga agacgcgatc
     2701 catcgtcaac tggacagcgg tgcaatagtg ctaatggggc cggtcgctgt ttcagtcact
     2761 ggcgagagct ttaatctgac ctcggaagag attgccactc aactggccat caaactgaaa
     2821 gctgaaaaga tgattggttt ttgctcttcc caaggcgtca ctaatgacga cggtgatatt
     2881 gtctccgaac ttttccctaa cgaagcgcaa gcacgggtag aagcccagga agagaaaggc
     2941 gattacaact ccggtacggt gcgctttttg cgtggcgcag tgaaagcctg ccgcagcggc
     3001 gtgcgtcgct gtcatttaat cagttatcag gaagatggcg cgctgttgca agagttgttc
     3061 tcacgcgacg gtatcggtac gcagattgtg atggaaagcg ccgagcagat tcgtcgcgca
     3121 acaatcaacg atattggcgg tattctggag ttgattcgcc cgctggagca gcaaggtatt
     3181 ctggtgcgcc gttctcgcga gcagctggag atggaaatcg acaaattcac cattattcag
     3241 cgcgataaca cgactattgc ctgcgccgcg ctctatccgt tcccggaaga gaagattggg
     3301 gaaatggcct gtgtggcagt tcacccggat taccgcagtt catcacgggg cgaggttctg
     3361 ctggaacgca ttgccgctca ggcgaagcag agcggcttaa gcaaattatt tgtgctgacc
     3421 acgcgcagta ttcactggtt ccaggaacgt ggatttaccc cagtggatat tgatttactg
     3481 cccgagagca aaaagcagtt gtacaactac cagcgtaaat ccaaagtgtt gatggcggat
     3541 ttggggtaag cgatgaaaat cgccggatgc gacatgcgta acactcgtgc gtcgcatcag
     3601 gcaattacgt ttattcccgc gaactaaaca atgccgccag accactgcgc cgctcagtgc
     3661 gcgtggcgat tgccgcactt aatatgcgct catcggcata cagcgacaga cggcgacgcg
     3721 cgcgggtcac tgcggtgtaa accagctctc gcgttactac cggcgtgcgt tggctcggta
     3781 aaatcaacgc cgcatggtcg aactccgatc cctgcgattt atgtaccgtc atcgcccacg
     3841 tcgtttcgtg ctctggcagg cggctcggtt gcacagactt aatattgccg tccggcatcg
     3901 caaaccaaac gcgcgtcccc tgcccgcgat ccagcgcaat accgatatcg ccattaaaca
     3961 acccaagcgc gctgtcatta cgggcaatca tcaccggtcg accttcgtac caacgagagt
     4021 gcggatgacg atgaattttg cgcttctgtt gcataaactg ctcaattcgc tcattcagtc
     4081 cagccacgcc aaacggccct tcccgcaggg cgcacaaaag ttggtactca ttgaacgcct
     4141 gaatgattaa atccggctcg gcacgcgctt gcagcagatc cagataacgt ccataacccg
     4201 caagagcttc ctcaagcatc gcaatataat cttctccgct ctgtaaaagt cgtttttcga
     4261 tatcagtaaa atcctgctga aaaacggttt tcactgccgt tttatcacca cggttgatcg
     4321 ccgcagctaa ctgaccaatg ccagaatcgc tgccgaaacg atagcttttt tgcagcaggc
     4381 agagactgtc gcgcaaagat gccgcttctg tgccggttcc tgccggaacg tgagtccccg
     4441 tcaggcggct tagctgcctg gcacgctcgg cggtaaagcc cgcgttggca taagcgcaga
     4501 tatcgcccag cacagcccca gcctcaaccg aggccagttg atcacgatcg ccgagaaaga
     4561 tcactcgcgc atgatctggc aaggcgtcga tcagtctcga catcataggc agatcgatca
     4621 ttgacgcttc atctaccacc agcacatcaa gatgcagcgg gttaccggca tgatgacgta
     4681 aacgctggct acccggctgc gcgcccagca atcggtgcaa agtgctggca tcttccggaa
     4741 tgcgtttctt ttgttcatcg gtcagcggta actgtcgcaa agccttgccg agagattcgg
     4801 ttaagcgcgc ggcagcttta cccgttggtg cagccagacg gatacggcag cgttcgccgt
     4861 cggccatttg aattaacgct gccagcaact tcgccacggt ggtcgtttta ccggtgcctg
     4921 ggccgccgga aaccaccgag atccgccgcg tcagcgccac tgccgctgca actttttgcc
     4981 agttaatttc atcgcttact ggaaaaagtt tgtccagggt ttgcgccagt agagcttcat
     5041 caacctcaat ggcatgattc acctcattga agaagcgcgc taccgtccgc tcgttacacc
     5101 acatgcgatt caaataaaga cgatcgccac agaggaccat cggcgtgggt tcatctcccc
     5161 tgctgaccgc ttgagaagcc agcaagcatt cttcccaatt ttgtagctca ccgatttcac
     5221 tgacacaggt cgccaacagc ggatgcgacg cctcgttatt ttccagtcgt gaaagcggca
     5281 aacagacgtg tccctctccg gcatcatgac ttaacagtgc cgccgcgagg gtgacggcag
     5341 gatgttcatc tcccgccacg gtcagggcaa attgcacatc cagcgggcgt agctgtttgt
     5401 gctccacagc ttccagtaat tgcttttgca atttcattac gcctcctcca gggtcatacc
     5461 ggcaaacatc tcatccatca gggcaatcaa cccggcgttg ggtcgggttg tgtaaatccc
     5521 ttgttgcgga tgttctttat caacgccacg caggaacaga tagataacgc cgccaaagtg
     5581 acgatcatag tcgtaatcag caatgcgatg gcgcagataa cgatgcagcg ccagggtata
     5641 aagctgatat tgcagatcat agcggtgtgc ctgcatcgct gctgccatag cctgttgggt
     5701 gtaagccgaa ctgtcttcac ccagccagtt ggatttatag tcgagcaggt aataacgccc
     5761 ttcgtggcgg aataccaggt cgataaagcc ttttaacatg ccgcgtacct gcatgaactc
     5821 cagcggcggg cagccagcgg atagcgggtc aaactggcga attagtgcat caagctggct
     5881 ggcgatgagc ggttcgctaa tcggcaggta aaactccatc tccacctgtt tatcgcggtc
     5941 ggaaagctga ctcaggctaa cacccgtttc attgagaggt gcctggagga cagccgtgat
     6001 ccactcggtc aacaccggtt cccactgcga ttcaaagccg ccgagttcca gttcttcctg
     6061 tacccagttc gggtcaaccg gctgggtaaa atcgaggtct tcaaacaaac tgtgcaagaa
     6121 tgtccccggt gacgcaccgc gcgggaactg atgcggtgtt aacgtcggtt cttcaacgac
     6181 gctgaccacg cctgcggcat caacatccag ccgtggcatc agatcctgag cgataccgtg
     6241 accacgctgc tgcaagccgg agtagctggt gacacgccag ttatcgccgg gcaatcgttg
     6301 tagcgtcctc gcattcagtt ctgcagttaa agcatcatta acctgccagg gctggttatc
     6361 accagtttgt gccgtttgcc aggcaatatc atcatcgcat aacgcttcaa tacaggtgcg
     6421 aagccctgcc gcatcttgcg gttccccttt ttgcagcaaa cgcccgagcg cactttggtg
     6481 gacgtcggtg tcaccttttt tatcgccacg acggcgcacc agcggtgcaa cgccgagact
     6541 gcaatgccaa accgaacgcg tcagcgccac gtaaagcaaa cgcagatctt ccgccagacg
     6601 ttcgacctcc gcaagctcga cgctttctgg cgcagcgtta agatccagaa cagcctcaaa
     6661 cgagtggcga tcgtgataaa acgcctgatc ctggacgcgg aaattggtaa taaatggcaa
     6721 ccagaccagc ggatattcca gccctttcga tttatggatc gtgacaatct gcaccagatg
     6781 tttatcactt tcgaggcgca tttgttggct ggaggcatta ctgtctggct cgaggatatg
     6841 ttgcgataac cagcgtacca gcgcatgttc actttccagc tgcgttccgg cttcttgtag
     6901 cagttcgctg atatgcaaga tatcggtaag acgccgctca ccgcctgccg ttgccagcaa
     6961 gttttctgca atgttacgcg ccgacatcag cgcccgcagc atcggcataa cgccacgttt
     7021 gcgccagatt tgccgataac catcgaactc ttcaactacc gcatcccacg catgttcgtc
     7081 attattcagc gtttcgatat ccagcgcgtt cagccccatc attgacgttg ccagcgcact
     7141 gcgcagggtg ttctcacgtt cgggcgtcat caccgcctgc aacaaccaaa gcatttcctg
     7201 cgcttccaga gtttcaaaaa cactgtcgcg gttcgaaagg taaacggaag ggatttccag
     7261 caacgttaag gcatcgcgca cctgggcggc ctcctggcgg ctgcgcacca gcacactgat
     7321 gtccgaagca cgcaccggac gcgcgtcgtc gccgttcatc agcaacgctt cgccccgctg
     7381 tccggcttgt agccagtcgc ggatttgcgc agcacatacc tgcgccatgg tactttgata
     7441 atcgccaacg ccgcagcttt cgccttccat cagccacatt ttcatcgcag gctgcgtttc
     7501 acctttaaac acaaaacgta acgcctgatt tttcccggct gatttcactg gaataaacgg
     7561 tatttcgcga aacatgaacg cgtcatcagt ctggctgaaa agcttgttca cactgttcac
     7621 cattccaggt gcggagcgcc agttggtgtc taaagtgtag tgggcgtgaa cttcactacg
     7681 cgccttcata taagtgaaga tatccgcacc ccggaatgca tatatggcct gcttcgggtc
     7741 gccaatcagc aacaatgcgg tttccggctg atggtgccag atacggcgaa aaattcggta
     7801 ctgctggggg tcggtatcct gaaattcatc gatcattgcc accgggaatc gcgtacggat
     7861 cgccgctgcc aacacctcac cgctttcgct acgcagcgcg gaatcgagcc gacttaacat
     7921 gtcatcaaaa cccaattcgc cacggcggcg tttttcacgc gctactgttt cgcggatctc
     7981 agccaatgcg cgggtgatca ccagatcgcg gatcgacaat ggttctgcaa gcagttgatc
     8041 gatcgcctca aacagtggat gtcgcggggt ttcccccccg gccttcgtgc gatcttctaa
     8101 gaaacgctgg gagaattttt ccagcgactc cggcaactga taactgtttg tctcttcttc
     8161 tgcccaggcg ctgatctttt cgatccattt agcctgattg ctacggttaa acttgcgtcg
     8221 atcaatacca gaagattcga tcagcgcatc aagttcaccc actgcgtcgc gccactgctg
     8281 ttttaccgta tcaatacgcg ccacaatttg cgcgtggcgg gaagccagcg tttcatcatc
     8341 gggcggcggt gctttgataa ccggtgcttc gccttgcaga taacgattaa tatcgcgcag
     8401 caacgcctgc ggccctttcc aggtttcaaa gaccaccagg gcaatttcac gcggcagcgg
     8461 gtagcagtgg cgacgccaga aatcggcgca ggcctggtag cgtagcagag actcatcttc
     8521 aatcagctgc tgctcaaaca gcataccgga ttcaaaggca ttcaggttga gcatgcgctg
     8581 gcaaaaaccg tgaatagtaa agactgccgc ttcatccatc tgccgttcgg ctaacaacaa
     8641 ccactgcgcg gcttgcgctt tatcgtcgat ctcttccagc aggcgttcgt acagtggatt
     8701 gtcggtggtt tcacgcagac aggcgatgcg caactcgtgg atattgctac ggatacgacc
     8761 acgcaattct gccgtggcag cctcggtaaa ggtgaccacc agcagttctt caacggtcag
     8821 cgggcgggga aaggcggcgg aaccgcctag tccaagtaac aggcgcaaat agagcgccgc
     8881 aatcgtaaag gttttgcctg tgccggcaga ggcttcaatc aggcgctcgc cctgtaaggg
     8941 caagcgcaaa ggatctagtg tctcggcgac atcactcatt cttttcactc atcaggggca
     9001 ttgtttgctg caacgcgctg acgttctccc acactttcca gccttcagga tgcacatatt
     9061 cggctttccc gttctggctg ccggaaatct gcgacagaat agccatgcct tgcggctcga
     9121 ccaccgcctg atggaagaaa tcagcaagtt tttgcggcgt cagcagtttt atctgggcca
     9181 cgattttatc acgcgaatcg aagcgcatat tgccgcgatc gaaatcttta cttaacttcg
     9241 atgcttcttc gccgagcgtt tgcggtgcct gcagcatctg ggtaattacc gcctgctgga
     9301 tttgcgcaaa ctcttctggc ttcatcgccc gcaattttgc ctctgcggtt gggaaaaacg
     9361 ccttgtaacg ctcccacaag aaggaaggct gtttatcatt gctttgcaaa aggaagccca
     9421 tgccccactg acgccccacg ctcattggaa atgcaaacac ggcatagccc aattgttctt
     9481 cggtacgcaa ctgattgtag aaccacggct gtacgatctg ccccaacaga gagctatagg
     9541 ctgagctggt gtattcatcg tagccagtcg gtacaaatac cgctgccagt gcggagtcgg
     9601 tgctgttacc agctttttca aagatgacgg attgtttttt atcgaccacg acatctttgt
     9661 tacgacacca ctctgaacca tcagcgccca actgtttttg cacatcgcgt gccagcgttg
     9721 ttgcctggtc ctcggtcatg ttgccgataa ccataaactc tggtcgagcc cctgatttta
     9781 aggcgtcgcg ataggccagc acctctttca acgtaatgga gggcaaaatt ttgcgccgtt
     9841 catctcgcga gaagtacggc acttgcgaga gcatctgcgc gggcataatc gcctgctcga
     9901 acgctttgcc cttttctgcg gaatccatca tctggttata ccaggacttc gcctgctcaa
     9961 gctgatcttc cgtggcggta tagctaaagt agccttccag caatgcctgg aacagctgcg
    10021 gcagatgctg ggtgtaacca ttagcattaa ccataaggcc gttgttagcg ttggtggaaa
    10081 aacttatgcc accaaccgac gcctggttgc ttaactgatc aagcgccagc cctgcgagat
    10141 aatcattgag cgcaaacatc acctgattgc gggcgctgtc catggctttc ggattacgca
    10201 aaatcaggct gacatcggct tttggctcgc tggcaaaata acggcttggc gcatacacca
    10261 cgcgcagatt cgactcatca acaatcagct ctggatggtc atatttcttg tctgacttaa
    10321 tcagcgagaa atcgtcagga atataagggt taagctctgg caaagagagc gcaatgtcgg
    10381 cggctttttt ctgccagtcg gcgaaagttt gtgcgctgat tttatcgacc tgatacggcg
    10441 catcgacaaa gtaagccgtt ttgttgtgcg gctctttcgg gctgatatac cagatacgcg
    10501 cattctgcgg cgtcatcatc gccagacgtt cctttactgc tttagcatcg taccgatcgg
    10561 caatattgac tgcatccagc gtatgctcaa caggaacgcg aatcatggta tctgccagcc
    10621 attcgacgta atccatatca cgggtgattg acggataacg gaagtcgata tccagcacat
    10681 tcgccagttc atcgaagtat tgtttatcga tccctttttc acgtaacaaa ttgaggtagc
    10741 taaaaatagc cgccacaacc tgatcgcgat tcgccagacc tttatcggtt aaagacgcag
    10801 agatcgctaa tacgccgctg ttgccgttga cgataggatc ggagttggcg ctaatgccct
    10861 caactaatcc ctgcttttgc agccagtcgg aaagtgtacc tgggctgcga ttaccaatca
    10921 gataggtaat caattcatcg gttttgctac ggaatttcgc cgagttgtta tcaatacgaa
    10981 actctacgcg caacacttta cgcggcagcg ccgggacgta atgaatgata atgccctttt
    11041 gcgcgtcggt gactaccggc acggtgattt ccggtttttt gctctctttg ttcggcacgc
    11101 gaccaaaggt gtccgccgcc atttttgcca actccggcag cggtttattg ctgtaaataa
    11161 ccgccttcat cagattggcg gaatagtact tctcgtggaa atctttcagc gcctgctgca
    11221 ccggattacc aggtttgtcg cttaaagttt cgaggttacc accagaaaac tttgaaccgg
    11281 ggtgtgccgg gttaatggtt tctgcgctga cctgtgccat gcgcatcccg tcacgcgtac
    11341 gcgccatggt taattcagcg ttcactgcat tacgctcacg ttcggcgtat ttcttgtcga
    11401 gcaagggttc tgcaatagca tcagccaggc ggtctaccgc accaggcaag gcgtcgttct
    11461 caacttccag atagaaagcc gtgcgatacg gcgcagtgct ggcattgtga ctaccgccgt
    11521 gcattttgag atattcggcc agactgtcag cctgcgggta ctttttcgac cccatcagac
    11581 tcatatgttc aaggtaatgt gccagcccct ggtacgcctc gggatcttcc agcgacccaa
    11641 cgggcaccac cagcgccgag agcgatttaa ctgcctgcgg atcagaaacc agcaagacca
    11701 ccataccgtt atccagacgt atagcctgat actggcggtt atctttatca cttttacgga
    11761 tggtttcctg aatcggctgc catcccgttt ctgcctgact taagggtgcc caaagggcaa
    11821 ctaacaacaa taatgctttg aaccaggtgc tgcggggcat tcacggacct cataatcaac
    11881 ttaattttct gtccagattc aacacgttaa cgcaaatcat ctgccagaat ttaatcttgt
    11941 gctgcacgag tcagcctatg tttatataac catcagtccg tgactggtgc gcatcataaa
    12001 gtaagcggat agattgcgca atttttatac agcactcatg actgattaaa gcgaaacagc
    12061 ggtaacagga aacgttgcga ctgttcaacg atggcctcca ttgtctctgg tgttaattgc
    12121 cgccagagcc tttgatacca gatatcatca ccttcgccac gcaccatcat gttgccttcg
    12181 taagcctgaa ggaatttcgt acgggctttt tgcaacgtgg aatcgtcatc cagcatggca
    12241 tcgttttgcg cgtcataaca ggtttttagc cacgcgccgc cactttcagg taacaccagc
    12301 aatggcgcgg acatcccttc acgatacccc tcaatcagtt gtgagaggta atgcaaagcc
    12361 tgttcggctg caagcggcgg aaaacgccac tcgccgtctt tgcgtagaaa aaggcgactt
    12421 tcaccattac caccgctggc acagtagaca aggtgttcca gccaaagttg cattccctgc
    12481 gccacactta ataaagaggg acgccagcgc aacaggccat ccggctgcac ctgcggcaac
    12541 cagccagtta tctgcacacc gttgcaggcg agatcaattt ccatactctg ccccggctgg
    12601 cgacaggcaa tgactctgtc ggcaagctgc tgcatctcct ggcactgtgt ttcccagaaa
    12661 atttcaccaa aagcgccata cggtaaatcc cctgccgccc ggaagcggcg gaacaagcgt
    12721 tcggcatcat cctgctcaac cagtgcattc aataactgct gattgatttg ataacggcta
    12781 agcccttcca gaataaatgg ctcggtgtct gggatttcgc tgtcttcagt acggaagttc
    12841 acctgcaaac gcatctggaa gaacgcccgc accggatgtg cccagaatcg ttgtagcgtt
    12901 tccagcggca cggtttccgg taaggtaaac ggcagcggct gaacaaattc agaatgtgct
    12961 ttaccagcct ggctggccgc aggtagccat tcacgagcat agctttgtcg ttcgcctggc
    13021 tggtagtttt gcggatcaaa cggcatccgg gtatggaggc aagtaagatg cgcttttacc
    13081 cttgcctcgc tttcatcaca gttgagcgct tcatcgtccg gtagataatg actttgcccg
    13141 atgtagtcga tcagttcctg caccagtacc gacgggaaac gttcactgtt atcctgaatg
    13201 gaacgcccga tatagctgat atagagtttt tgctgcgcgg aaattaacgc ttccaggaac
    13261 aggtagcggt cgtcatcgcg acggctacgg tcgccacgct tcggtttctg gctcatcagg
    13321 tcaaagccca atggcgcaag ctgacgtgga taaacgccgt cgttcattcc cagcaggcaa
    13381 accactttga acggaattga acgcattggc atcagagtac aaatgttaac cggtccggcg
    13441 agaaaacgct ggctgatacg ttcttgatcc agacgctgcg ccagttcatc acgcaatagt
    13501 gacagcggca ccgcgtcgcc atactgcgca cctaaacctt cggcgataat cgcctgccat
    13561 tgttgttcga tcagcgtcat cgccgcttcg gtttccgcat ccggcaggaa gaaggcgttg
    13621 agcatatcgc gacaaaccgg caaccactct tctagcggac gctcctgtgc cagcccgcga
    13681 cgccagatgt ttagctgcat tagcagtgaa gccagatgcc ccaccagttc tgcaattaag
    13741 ccgctcgatt catcataagg tagaaccgat tgccactcgc cctgcgcgct ctccatcgcg
    13801 tagcccagca acatacgcgt caggccaaat cgccaggtgt gttgtccggt ggcggggagt
    13861 tccagctcgc gaacgttgtc gtcatctatc ccccaacgaa tgccggattc gttgacccac
    13921 tggcgtaaat aacgcagccc ttcttcggtg atgtcaaacc gcgccgccag caccggcaca
    13981 tccagtaatg ccagcacgtc ttccgacaca aagcggctgt ctggcaacga taacaggctg
    14041 ataaacgctt ccagtaccgg atgcgactgc cgcgcccgac ggtcggaaat ggcgtaaggc
    14101 aggtaacgat ccgcaggtgc gctaccaaac acagcctgaa taaacggact gtagctgtcg
    14161 atatcagcca ccatcacgat gatgtcgcgc ggagtaagtg tcgggtcttc ttccagcatc
    14221 gccagcaggc gatcgtgcaa aacttcaact tcacgctgcg ggctatggca aacgtggaag
    14281 gtgatactgc tatccagtgg atcaagcggg cgtttgttat cgctacggga aaactcttcg
    14341 atgttcacac cagcaacggc acggttttcc agttccagaa tgtcagactg aatgttatgc
    14401 agcaggttat ctggcgtgac atcgacaaag gcgtccagct cctggctgct ctccaggtca
    14461 gaaaggagat aaatgtagtc gcgcccaagc ttaccccatg aagccagcag cgggttgccg
    14521 acatcctgtt caccatcgct gttaaagagc tgcccggcat tttcgctatc acgaaatagc
    14581 ggcaattcgc gatcttcaac actgtgtcga cgctgacggg tcagcagttt cgccagataa
    14641 gcaggatctt taatatcgcc ccagtaataa cggcaggggt tggtaaacag gagatggatt
    14701 tcaatatgtt tacccagcgc ctggagcgcc tggagataaa caggcggtaa cgcggaaata
    14761 ccgcatataa agacgcgcga aggtaacccc ggcgggcagg tcgtcgcgga ctccagcgtt
    14821 tcgataaagc gctgatagag attggcgcgg tgccaacgcg gttgtccgag ttcatgggta
    14881 tattccacca gcgccttcca caacggcgct tgccaggcct gtgcttctcc cagcccttca
    14941 accaaatgtc ctgtttccca ctgtgccagc cactccgggc gatagaccag atactggtca
    15001 aacaggtccg ccgcttttga ggaaagctgg aacagttttc gcttgtcgct atcgtcagcc
    15061 agataatgcc gcaacagggt aaagtcttcg cgatccagca attgcggcag cagagtcatc
    15121 agtttccagc tcatgctctg tttgttaaag gcgctctctt tggggatctc cggtaacacc
    15181 cggacgaaca tatcccagat aaagctcgct ggcagcggaa aatcaatgtt tgccgcaata
    15241 ccaaactttt gcgacagggt catttgcagc cactgcgcca taccggtact ttgcaccaga
    15301 atcatctctg gttcgaaagg atcgtccagc cgttcgcgtt cgacaataaa ctccatcaac
    15361 gcttccagca cgtccagacg attggaatgg tagaccctta acatagcggc tcctgactac
    15421 tgacgattcg ggcaatgcag gcgggtcatc tcgccttctc tgcccccggg tgaaactagc
    15481 gtaacgctga tgctgacaca tcccgcctgc gatgtctgca ttcggttgac ctgccagttg
    15541 gcaggtggcg aaatcgcgcg cagttgcgtt tgctgccaac catgccgcca gagctgttgg
    15601 tactggtttc tgctggcgag actgttcatt aatgttcgct ggatacccga taacgcagtg
    15661 acaatcatca ccatcagcac catcgcaacc attacctccg gcaggctaaa gccttgttga
    15721 ttcttcaggg aagctgacat aacgccctct ctttcagcgg acaaaaatcg ctccagccgc
    15781 gtggcgaaaa aacaatgttt ccatcaatga cttcgcctgt tcgccacaac gaaacgcctt
    15841 cataaccggc aaccaataag gcttcattat ctgccagtaa acgcaaacaa acccgggcat
    15901 cggttccagc gtactgcgag cactgaactg cgggctgcgt ctgccaggag tgcattttcc
    15961 cccacgccag cgccgactga acgatggcct ggcggcgcaa tgactgactt tccatgctca
    16021 cgcgagaggc aaaactgcga tcctgctgac tcattccttg caatagcaag ctacccaaaa
    16081 ccagcagcat caggaccaga gccagtgacg aaacaccttt ttcgcggttc acaggttgaa
    16141 tcctgtcacg ctatagctgg catccaccac ggtttgcggt tcagacttac tggcagcacg
    16201 catattaacc gtcaacaccg gcgagaagcc gctgacatcc tgacgtacga cctgaaaagt
    16261 gtcgataatg atggcatccg gattagtgac tttatcccag cccttacctt cacaggatgt
    16321 cgcaccgcgt agcgtttcca gcacatgctc cttcagacga aatccaatct ggtcagactc
    16381 ttttaccggt tcgcgatccc agataccgtt actgttcgca tcccactgca caatgataca
    16441 gtcaccctgt ccgacaattt ccagcccttc gccggtacag ctgccatgac aataacccgc
    16501 cctctggaga tgcttcgcga cggtaaatac ccgcagccag atttcatctt ccagcgccag
    16561 cttacgggtg ctcgttaaac tttcacgctg taacgcaggc agaaagcgtg cagcccccag
    16621 caacaatacg ctactgatcg ccatagcaat caacacttcc agcagagaaa aaccttgctc
    16681 ttttacaggc atccttctgt ttctccttgc tgacaaagcc ggagtcttcc ccacggcgaa
    16741 accaccagcc accactcgcc cgttgagttt ttgaagcgaa tatgcccggc ccatgcggta
    16801 ttgcgcaggc caaagaaagc aagcgaaggt gtcaggtcgc tcatttcgac ttcgggccag
    16861 cgtggcacaa agaccaatgg tgaactgcca tgacaggtat tggccccagc agcggaactc
    16921 acaaggcacc ataacgtccc ctccctgata acgctgatac tgtggtcgcg gttatgccag
    16981 ttggcatctt cacgtaaata gagcaaatag tcccgcgcct ggctggcggt ttgccatagc
    17041 cgttgcgact gctgccagta ttgccagcca tagagtccac ttgcgcttag catgaccaaa
    17101 atcagcatcg cgaccagcgt ttcaatcagc gtataaccac gttgtgtttt catgccggca
    17161 gtatggagcg aggagaaaaa aagacgaggg ccagtttcta tttcttcggc gcatcttccg
    17221 gactatttac gccgttgcag gacgttgcaa aatttcggga aggcgtctcg aagaatttaa
    17281 cggagggtaa aaaaaccgac gcacactggc gtcggctctg gcaggatgtt tcgtaattag
    17341 atagccaccg gcgctttaat gcccggatgt ggatcgtagc cttcaatctc aaagtcttcg
    17401 aaacggtagt cgaagatgga ttcgggttta cgtttgataa tcaacttcgg cagcggacgc
    17461 ggttcgcggc ttaattgcag atgagtttga tccatatgat tgctgtacag gtgcgtgtcg
    17521 ccccccgtcc agacaaaatc gcccacttcc agatcacact gctgcgccat catatgcacc
    17581 agtaacgcgt agctggcaat gttaaacggc aggccgagga aaacgtcaca ggagcgctga
    17641 taaagctggc aagagagttt gccgtctgcc acatagaact ggaagaacgc atggcacggt
    17701 gccagcgcca ttttatccag ttcgcctacg ttccacgctg aaacaataat gcggcgcgaa
    17761 tccgggtcgt ttttcagctg gttcagtacc gtagtgatct ggtcaatatg acgaccatcc
    17821 ggcgttggcc aggcgcgcca ctgtttacca tacactggcc cgaggtcgcc gttttcatcg
    17881 gcccattcgt cccagatggt gacattgttt tcgtgtagat aagcaatgtt agtgtcgccc
    17941 tgcagaaacc acagcagttc atggatgatg gaacgcaggt ggcaacgttt agttgtcacc
    18001 agcgggaatc catcctgcag gttaaaacgc atctgatgac caaaaatgga aagcgttccg
    18061 gttccggtac ggtcgttttt ctgtgtgcct tcgtcgagca ctttttgcat cagttctaaa
    18121 tactgtttca tggttcctca ggaaacgtgt tgctgtgggc tgcgacgata tgcccagacc
    18181 atcatgatca cacccgcgac aatcatcggg atggaaagaa tttgtcccat gctgatgtac
    18241 tgcacccagg caccagtaaa ctgcgcgtcg ggctggcgga aaaactcaac aatgatgcga
    18301 aacgcgccgt aaccaatcag gaacaaacct gagacagctc ccattgggcg cggtttacga
    18361 atatacaggt tgaggataat aaacagcact acaccttcca gcaacagctc gtaaagctgt
    18421 gatgggtggc gcggcagcac accgtaagta tcgaaaatgg attgccactg cgggttggtt
    18481 tgcagcagca aaatatcttc tgtacgggag ccagggaaca gcatggcaaa cgggaagttc
    18541 gggtcaacgc ggccccacaa ttcaccgtta ataaagttgc ccagacgccc ggcaccaaga
    18601 ccaaacggaa tgagtggtgc gataaaatca gagacctgga agaaggaacg tttagtacgg
    18661 cgggcgaaga taatcatcac cacgataacg ccaatcaggc cgccgtggaa ggacatgccg
    18721 ccgtcccaga cacggaacag atacagcgga tcggccataa actgcgggaa gttgtagaac
    18781 agaacataac caatacgtcc accgaggaag acgccgagga agcccgcata gagtaagttt
    18841 tcaacttcat ttttggtcca gccactgccc ggacgattcg cccgtcgtgt tgccagccac
    18901 attgcaaaaa tgaaacccac cagatacatc aggccgtacc agtgaagcgc cacgggtcct
    18961 attgagaaaa tgaccggatc aaactccgga aaatgcagat agctactggt catctgtcac
    19021 cacaagttct tgttatttcg ctgaaagaga acagcgattg aaatgcgcgc cgcaggtttc
    19081 aggcgctcca aaggtgcgaa taatagcaca aggggacctg gctggttgcc ggataccgtt
    19141 aaaagatatg tatatgatcc gcgctataac cctccgcgaa tcagcccgcc catgccacga
    19201 cgctccataa aggctgcaac ctgatggcga acttcggtcg ccagttgcgc ttccagacta
    19261 cgctgcgcaa gattttctgc ttcggcataa tcaatgcgcc gcagcaggta ttttacccgc
    19321 gctacagaac gtccgttcat agacagatgg cgatacccaa gcccaatgag gattgccacg
    19381 cacatgggat cgcccgccat ttcaccgcac aaacggagat cgattccatg tatttccgct
    19441 tcccgggcga tcatcgccag agctcgtaac attgcaggat gaagactgtc ataaatgttc
    19501 gccacccggg tattgttgcg atcaacggcc agaatgtatt gagtcagatc gttggtgcca
    19561 acagagatga aatcgacccg ctttgccaga tgcggcagca taaataccat tgacggcact
    19621 tccagcatga tgccgatacg tggtttggga atttcgtaac cgatcatctc ctcgacttca
    19681 cgtccggcac gttcaatcag gcggcgtgct tcatcaactt catcgaggct tgtgaccatc
    19741 ggcaacagaa tattcaggtt gcccgtagcg gcattagcac gcagcatcgc ccgcacctgg
    19801 atcaagaaga tctccggctg atcgagcgta atgcgaatcc cacgccaacc caggcatgga
    19861 ttctcttcgc tgatcggcat gtaaggcagc tgcttatctg ctccgacatc cagcgtacgc
    19921 aaggtgacgg gtttatcatt gaacatttgc agcatcccct gatactgcgc cacctgttct
    19981 tcttccgacg ggaaaccact ttgcagcatg aatgggattt cagtgcgata aagaccgatg
    20041 ccatcaatac ggctgcccag tttttcttca tgttccgggc ttaaaccagc attgagcatg
    20101 acttttatac gctcaccgct ttttaactgc gcgggtaaat tgacgtcatc ttccgccaga
    20161 cggctaagct caatctcttc actaattagc cgctgatatt cttgcagcag taccggctcc
    20221 ggatcgacca gcaattcacc gcgatagcca tcaacgatca gcgtcctacg atgcagcacc
    20281 gaaggctgaa tatccgcgcc catcacggta gggatcccca gcgcacgtac catgatcgca
    20341 gcatgggagt tggctgcgcc atctcgcacg acaacaccga ctaagcgatc ctggggcagc
    20401 tcagcaagag ttgtcgctga cagttcatct gccaccagaa tgaaacgttc cggccaggcg
    20461 ttcggccctt gattagcgtc atcaagatga aacagcaatc gctgacccag cgcacgtaaa
    20521 tcgccagccc gctctttgag atagttatcg cttagcgcgg caaactgttc ggcaaatttt
    20581 tcaatgaccg tttttaccgc ccactctgcc accgagcctt tatcaacctc ggcaaacaat
    20641 tcgcgacgca gccgggtgtc cgaaagtagg tgcgagtaaa gatcgaaaat agccgccgtt
    20701 tctttttgtg ctccggcggc aaagcgtttg ctgtagcggc gaaactcgtt tgccgcttct
    20761 tccagcgccc cggtcagtcg ttcgcgttcc agagccggat ccagcgttga tgcctgatac
    20821 acctgttcca ttaaaggtaa cgtggcatcc tgccagcctt cggcaatcgc cacaccaggt
    20881 gctgccggta atgcgcggat tcgcgtctgg cgatattgcc caaacagggc agtcaactgc
    20941 gactgggaaa gaatagctgc catctgggtg gcaagcgtca ccaggaagga ttcttcactt
    21001 tcgtcatact ggcgcaactc tcgttgctgt accaccagta caccaagcaa ctggcgacgt
    21061 tgaataattg gtacgcctaa aaacgcgcgg aaacgttctt cttttacgga ggggatgtat
    21121 ttgaagctgg ggtgcttttg cgcatctgca aggtttatcg gttccgccag cctgccaacc
    21181 aggccgacga tcccttcatc aaacgcgagc gttacagtgc gaccgcgtgg ttttttcagc
    21241 ccccgggtcg ccatcaggta gtaacaacgt cgatcatgat cggccaggta gaccgaacag
    21301 acctcggtat ccatcgcaag acagatgtcg gtaaccagaa tatttaacgc ctcattcagg
    21361 cgtggtgcgc tggctacctt ttcgactatt tcgcgcaggc gagtgagcat aattggcgtg
    21421 acttaacctc ttttacgtcg ataagcagat gcgttttgtg gttttggcgt attttcctgc
    21481 agtgacatca ccacactcgc gaactctttc attaccctac ggtagacatc acgtttaaat
    21541 gacaccacct gtctgaccgg ataccagtaa cttacccatc gccagccgtc aaactctggt
    21601 gtactgctgg tttgcatatt gatttctgca tcgccgctta ccagctgcaa gagaaaccat
    21661 ttttgttttt ggccgataca aaccggcttc gtgtcccaac gcaccaaacg tttcggtaat
    21721 ttgtagcgca accagttacg cgttgaagca aggattcgaa cgtctttgcg gcttaatcct
    21781 acttcttcaa acaattcacg gtacatcgcc tgctctgcgg attctccggg gttgattccg
    21841 ccttgcggaa attgccagga gtgctgacca aatcgccggg cccacattac ctgcccctgg
    21901 cgattacaaa tcacgatacc tacgtttggg cggtagccat cgtcatcaat caccggacta
    21961 cctcaaaata aagctttata tacgaatgat tgtttcatac tccaggaaga cggtaaacca
    22021 ctctctgcag ggcattacac actaataaca attgaataac tcacagttat gtgcagagtt
    22081 ataaacagag gaaggggtgg atagccgttt ttattcacct tttctgtgga tagagttgtg
    22141 aagaactacg gaattactac gggaaaaccc ggagcattct gaataagccc gatatagaca
    22201 ttttaaaata ttcattatta gtcaatgcat tacatgtaat ttgagtaatg aaaaagagta
    22261 attcgtgacc cagctcacac ctctcattta cgggttggcg aaagatcaac caatgccgta
    22321 tttatccaca gaatgtgcca ctaagttaag cactgaacca ctaaaaactg gagtttcgtc
    22381 gcacgtcaag gctgtaaatg gaaacagtag tggaggtttt tcacagttat cccagctttc
    22441 tgtggataac atggtgtaag atcctgttta ttttcagtga ccagatttgg aaaacctatt
    22501 gcagtgttgc gcaactcgat taccggcagc ctaaaaagca atataaatca gagaattgaa
    22561 caacacatat ggaaaaagtt acactgcgaa tattcggcac ataattgctg tttgtttttt
    22621 aaccaaggta tcatgacatg tcccaacctc gcccactgct ctctcctccc gaaactgaag
    22681 aacagttgtt agcgcaagca cagcaacttt ctggttatac attgggagaa ctggcggcac
    22741 ttgccgggct ggttacgcca gagaatttaa aacgcgataa gggctggatt ggcgtgttac
    22801 tggagatctg gctaggtgcc agcgcaggga gtaaacctga gcaagatttt gctgctctgg
    22861 gcgtggaact taaaactatc cctgtggata gtcttggtcg tccgctggaa acaacattcg
    22921 tttgtgttgc cccgttaacg ggtaatagcg gggtgacctg ggaaaccagc catgtgcgcc
    22981 acaagctcaa acgcgtactg tggataccgg ttgaaggcga gcgcagcatc ccgctggcgc
    23041 agcgtcgcgt aggatcaccg ttgctgtgga gcccgaatga agaggaagac cgacagctac
    23101 gcgaagactg ggaagaatta atggatatga ttgttctcgg tcaggttgag cggatcaccg
    23161 ctcgtcacgg ggaatattta cagataagac cgaaagcagc gaatgcgaaa gcgcttaccg
    23221 aagccattgg tgcccggggc gaacggattc tgacgctgcc acgcggcttt tatttgaaga
    23281 agaatttcac cagtgcacta ctggcccgtc attttctgat ccagtagcca tcgctttgac
    23341 ctgccgcttt ccgggcatat aattaccgct tcattttttt ggcagggctt tttagatgtt
    23401 atttgcatgg ataaccgatc ctaacgcctg gcttgcgctc ggtacgctga cgctgctgga
    23461 gatcgttctt gggatcgaca atattatttt cctttctctg gtggtggcaa agcttcccac
    23521 agcacaacgt gctcatgcgc gccgtctggg gttggcggga gccatggtta tgcgtctggc
    23581 gctgctggca tcaatcgcct gggttacgcg cctgacaaat ccgcttttta caatattcag
    23641 tcaggaaatt tctgcccgtg atttgattct gcttctgggt ggcttgttcc ttatctggaa
    23701 agccagcaag gaaatccacg aatccattga aggtgaagaa gaagggctga aaacacgcgt
    23761 ttcatcattc ctcggcgcta tcgtgcagat tatgctgctg gatattatct ttagcctcga
    23821 ctcggtgatt acggctgtgg gtctgtcaga tcacctgttt attatgatgg cagccgtggt
    23881 gattgccgta ggcgtgatga tgttcgccgc gcgctcgatt ggtgattttg tcgaacgcca
    23941 tccttcggta aaaatgctgg cgctctcttt cctgattctg gtgggcttta ccctgattct
    24001 ggaaagtttc gacatccacg taccgaaagg ttacatctac ttcgcgatgt tcttctctat
    24061 tgcggttgaa agcctcaact tgattcgcaa caaaaagaat ccgctctgat actccgtacg
    24121 ctctcctgcg ggagggcgta ctcactcctt ttaccctcac agtttaagat tttcctgctt
    24181 tcaagatata taacgtcggt ttataaacag actattatca taggtaggcc aaatgaggat
    24241 aaccggatga aaaaatgggc agtaataatt tctgcagtcg gactggcgtt tgctgtttcc
    24301 gggtgttcca gtgattacgt catggcgacc aaagatggcc gtatgatttt gaccgatgga
    24361 aaacctgaaa ttgatgatga taccgggctg gtgagttatc acgatcagca aggtaacgcg
    24421 atgcaaatta accgtgatga tgtttcgcaa attattgaac gttaacaaat aaggtcagca
    24481 tccggctggc cttaagattt ttctctttcc cttttccctt ccctctgcca tttttatatt
    24541 ccttatgtcg tgattataaa aaggaaacgg ctatgcaata tcaccgtata ccccacagtt
    24601 cgctggaagt cagcacgctg gggcttggca cgatgacgtt tggtgaacag aacagcgaag
    24661 ccgacgccca cgcacaactc gactatgccg tcgctcaggg cattaacctt atcgacgttg
    24721 ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg
    24781 gcaactggct ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg
    24841 gaccgtcgcg caataatgac aagggcatcc gcccggatca ggcgctggat cggaagaata
    24901 tccgcgaagc gctgcatgac agcctcaagc gcctacagac tgattacctc gatctttatc
    24961 aggtgcactg gccgcagcgc ccgaccaact gcttcggcaa actcggttat agctggacgg
    25021 attctgcgcc tgcggtttcg ctgctggata cgctggacgc actggcagag taccaacgcg
    25081 cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc atttggcgta atgcgctacc
    25141 tgcatctggc ggacaaacac gatctgccgc gtattgtcac cattcagaac ccttacagtc
    25201 tgttaaaccg cagttttgaa gtaggtctgg cagaagtcag ccagtatgaa ggggtcgaac
    25261 tgctggccta ttcgtgcctg ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa
    25321 aacccgctgg cgcacgtaat acgctcttta gtcggttcac ccgctatagc ggtgagcaaa
    25381 cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg tcatggcctg gaccctgctc
    25441 agatggcgct cgcgtttgta cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa
    25501 ccacgatgga tcagctgaaa actaacatcg aaagtttgca tctggagtta agcgaagacg
    25561 tattagctga aattgaagcg gtgcatcagg tttatactta tccggcacca taagattaac
    25621 gccggttaaa accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat
    25681 tgccagcgca aacagcgcac cgaagccaat gccaatgggc acgaccggga tgcctatcat
    25741 taccgccagc gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg
    25801 tactgcaatc gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt
    25861 gagcggaacg acaaaaaagc cccccatcac gccaatcagc atcaacaagg cataggctgg
    25921 cagcagctcg tgttgcaggg aaaaaatcag taccaccacg ccaatcaaaa tcccggctgg
    25981 catacagcgt gacacggttt ccagcgtaac taacttcgct gccgcacctg cgccaaccac
    26041 gataccaatc gctaccatcg cgttgagata ggtgggcgta gcgttatcgg taatgcccag
    26101 cgccaccggt acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa
    26161 actggtgccc accagcgaaa aacgcgtttc accattgcgc catagcgagg tgcaggcatt
    26221 caggaaactg cgggtcatgt tgatgagatt ccaggactgc cccggacgcg ccgccgccag
    26281 tttgggaatg tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag
    26341 ggcgacgagg acatgccagt cagccagcac accaccggct acggaaccga gcaaaatcgc
    26401 cgctatggta gaagcttcca ttaaaccgtt agctttcact aacttactac ccgtggttaa
    26461 ttcgccgaga ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt
    26521 atagccgaga aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc
    26581 gttggcaaac atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg
    26641 cgcaaaaaga atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg
    26701 atagaactgc gctttcagta acgccagagt ggcaaacagt agggcattat cgccaaacgc
    26761 agagagaaac tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt
    26821 gtgcactgac tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa
    26881 agtcaggttt gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta
    26941 cagcaagctc cggcacgccg tgctcgcggg catactgttg caacttatcg cgcgtcagtt
    27001 cgttatctgt ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag
    27061 tggcatggac tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca
    27121 ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct
    27181 gctcgtcaaa acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa
    27241 cattctcggc ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca
    27301 ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc gataccagga accgacaaca
    27361 ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta
    27421 cgttgataga aacgacaggc gcgcactcgg ttacaccata gccttcaagg atgcgcaggc
    27481 caaatttatc ctgccaaagc tgtttggtac tttcttgtag tttttctgcg cctgccacca
    27541 catagcgtag acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag
    27601 tcgaggtacc gaacaacacg gtgcaactgc ggtcatacac caactccggc acaatgcggt
    27661 aatgcagcgg gcttggataa aggaacactt ctgcacctgt aagcagtggc gtaaacaggc
    27721 ctaccgtcag cccaaaggag tgaaacagcg gtaatgccga cataaagcga tcgttggtgg
    27781 tgaagtcggc aatcgtttta atctgctcga cgttcgccag aatgctttta tggctatgga
    27841 cgacgccttt tggatggcct tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg
    27901 gctgctgttt tacctgcgcc aggcgcggca tcagcaaatg agcgaagatc catactttgt
    27961 cggcagtggt gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt
    28021 gctccggcag atgccagagt ttgcctttat cgagaaactg gcgggaagtg aagatggttt
    28081 tgatttcagc cgccgtaata gcactggtca gcccttttac cccagcagtg tagttcatca
    28141 ttgcgggaat gcggcgacgc gcgatggccc caaaaatcac tgccgcactg atgcctgcat
    28201 tgggcagcat taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa
    28261 caaacagcgt tttcgtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga
    28321 cacatttctt cccggctccg aagcggtaca ttgcactcag taaagattcg tacagagttt
    28381 cacgcgggcg caccgccata cgcgcttcca tcattatttg atgcagcatt tcgccagcga
    28441 ttttgcgacg gtcacgggca cgcggcgcat ccggcatcgc cacctgcgtt ggtggcaaaa
    28501 tatgcagagt aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt
    28561 gcgtaagttc cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga
    28621 caaaacccgc gccatcgtag attttcatca gcgagcctgt agtggtgatg cgtccttcag
    28681 ggaaaatcac cactggtcgg ccctgttcca ccagacgtac cagatgttta atagccatag
    28741 gttgcgtcgg gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact
    28801 gttggcttat tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca
    28861 aaatgccatc aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg
    28921 cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc
    28981 taaaaagcat tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca
    29041 cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc
    29101 aggttggtgc aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct
    29161 ctgggatctt gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat
    29221 gaagggaaat gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca
    29281 aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac
    29341 ttcacctatc aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc
    29401 ggtcaatcgc aacagcgttt cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc
    29461 ttacttattt gcgtttggct cgcattccca cgatgaaaac acgccacccc ttgaaccaac
    29521 gggcgttttc cgtaacactg aaagaatgta agcgtttacc tactaaggta ttttcatggc
    29581 gaccataaag gatgtagccc gactggcagg cgtttcagtc gccaccgttt cccgcgtcat
    29641 taataattca cctaaagcca gtgaagcttc ccggctggct gtgcatagtg caatggagtc
    29701 tcttagctat cacccgaacg ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt
    29761 cggtctgatc gttggtgatg tttccgatcc gtttttcggt gcaatggtga aagcggtcga
    29821 acaggtggct tatcacaccg gtaatttctt attgattggc aacggttacc acaacgaaca
    29881 aaaagagcgt caggctattg agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca
    29941 tgccaaaatg atcccggatg ccgatttagc ctcattaatg aaacaaatgc ccggtatggt
    30001 gctgatcaac cgtatcctgc ctggctttga aaaccgttgt attgctcttg acgatcgtta
    30061 cggtgcctgg ctggcaacgc gtcatttaat tcagcaaggt catacccgca ttggttatct
    30121 gtgctctaac cactctattt ctgacgccga agatcgtctg caagggtatt acgatgccct
    30181 tgctgaaagt ggtattgcgg ccaatgaccg gctggtgaca tttggcgaac cagacgaaag
    30241 cggcggcgaa caggcaatga ccgagctttt gggacgagga agaaatttca ctgcggtagc
    30301 ctgttataac gattcaatgg cggcgggtgc gatgggcgtt ctcaatgata atggtattga
    30361 tgtaccgggt gagatttcgt taattggctt tgatgatgtg ctggtgtcac gctatgtgcg
    30421 tccgcgcctg accaccgtgc gttacccaat cgtgacgatg gcgacccagg ctgccgaact
    30481 ggctttggcg ctggcggata atcgccctct cccggaaatc actaatgtct ttagtccgac
    30541 gctggtacgt cgtcattcag tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag
    30601 cgactaaccg cagttaaagc aattccagcg ccagtaattc ttcgatggtc tggcgacggc
    30661 gaatcaaccg cgcctgacca ttatcaaaca gaacttctgg taacagcgga cggctattgt
    30721 agttggatga cattgatgcg ccatatgccc ctgtatcatg cagtaccaga taatcacccg
    30781 ccttcacttc cggcaaggcg cgggtttcaa catttccccc ttcctgctgg gtaaagacat
    30841 cgcccgattc acataatggc ccggcgacga cggtttccac cgttggcgcg tgttccagag
    30901 aacgaccatc agctgccagg gcactgatat ggtggtaact accgtacatt gccgggcgca
    30961 tcagatcgtt gaacccggca tcaaccagca caaagtggcg gctacccatt tgtttgacgc
    31021 tccgcacctg agtaattaac acgccagact gcgctaccag aaagcgaccc ggttcaattt
    31081 ccagtttcac agggtggccc aaatggcgag cgatttgctc acgcgcggca ttccacagac
    31141 cataataatg ttcggtatca accgcctctt caccctgttg ataaggaata gaaagcccac
    31201 cgcccgcaga aatagcctgt aaatcctgac cgaattcgat gacctgacgt accatagcac
    31261 cacacacctg ttccagatgg gcataatcaa cgccagaacc aatgtgcatg tgaatgccga
    31321 ccagctgcaa atgatgacgt tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga
    31381 taccgtgctt gctgttttcg ccaccggtat tggttttttg gctatgtccg tgaccaaacc
    31441 ccgggttaac gcgcagccat acccgatgcc ctggcgaaac ctggcccagt tggtcgagca
    31501 tatcaacaga acccgcattc accggaattt gcaattcact gacgcgttca agcgtcgcct
    31561 gatcgataac atctgccgta aaaacaatat catcggggtg cgtttgcgga ttgtaacccg
    31621 ccgccaacgc acgctctatt tcgcctaacg agacggaatc cactttcacg ccctgctcac
    31681 gcattaatcg caaaatatga atattggaac aggctttctg tgcaaagcgc accacatcaa
    31741 actgtttcag cgctgcaatc tgccgacgaa taatttgcgc atcgtagacc cacaccgggc
    31801 agccaaattc agcgggcaaa cgcagcagat tttcggcggt gagatcggta tcggtgctga
    31861 acagtgaatg tggcataaca aactccagat gagtgctttt ttatgattac gccacatcat
    31921 aaaaagaata aaaaatatcg atttatgtcg agtctatgca aaaatgatat ggattaccgg
    31981 attgcgagag agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg
    32041 taatgaccgc cggaagcctg actgaggcag cacacctgct acacacctca cagccaaccg
    32101 tcagccgcga actggcgcgc tttgagaagg tgatcgggct aaaattgttt gagcgcgtac
    32161 gtggacgatt acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct
    32221 ggtacggact ggatcgcatt gtcagcgccg cagaaagtct gcgcgagttt cgccagggag
    32281 aactgtctat tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac
    32341 cctttctggc acgttatccc gatgtcagct taaatatcgt gccccaggaa tcaccgctac
    32401 ttgaagagtg gctctcggcc cagcgtcatg atttaggact cactgagaca ctccatacgc
    32461 ctgcgggaac agaacgtacc gaattactct ctttagatga agtgtgtgtg ttacctccgg
    32521 gtcatccgct ggcggtaaaa aaggtattaa cgccggatga ttttcagggt gagaactaca
    32581 tcagcctttc ccgtactgac agctatcgcc agttgctgga tcagctattt actgaacatc
    32641 aggttaaacg acgcatgatc gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac
    32701 gggcgggggt aggtgtttcg gtggttaacc cgctcaccgc actggattat gcggcaagcg
    32761 gtttagtggt acggcggttc agtattgcgg ttccgttcac cgtcagcctg atccgccccc
    32821 tgcaccgccc gtcatcagcg ctggttcagg cgtttagtga gcatttacaa gcggggttac
    32881 cgaaactggt cacttctctt gacgcgattt tgtcgtcagc tacgacagca taaaagcgac
    32941 agcatcctcg gcatggatcg ccgcagtatc aaacacaggc aaaacactgc gctcttctgg
    33001 aaccaataaa ccaatttctg tgcagccaaa aatgacaccc tgcgccccct gttctgcaag
    33061 gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag
    33121 ttcttcaaaa ataatctgat taattttcgc ccgttcatcc gcttcaggaa taagacaatt
    33181 gatggaaaat tgttccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt
    33241 acccagcagc gccacacgag tcattccggc cccggtaatt gcacgtccgg tggcatccgc
    33301 aatgtgtaag aaaggcagag tgcaacgtga ctcaatggca tccgccactt tatgcatcgt
    33361 attggtacat aacacaatac cttctgcgcc cgcccgctgt aagccaagcg ccgcctcagc
    33421 cagaatgtcc ccggttttat cccattcccc gcgacgctgg cactcttcta tttcatgaaa
    33481 atcgacgcta tgtagcagca cttgcgcaga gtgaagccca ccaagccgct gtttaatgcc
    33541 ttcatttatc agacgatagt aaggaatggt ggattcccag ctcattcctc ccagcaaacc
    33601 aattgttttc attcctctct cctgatgtgt gttaccgcaa caaggctgaa taaggaacga
    33661 gacaaacgcc tcaacggcca ggtgcccgat ctctattaac gaaaaaaggg ccggatgtac
    33721 agcacatccg gcccgtgaaa tcagacgccg atatttctca acttctcgcc tgccatcagt
    33781 ttgcgttcga tatgttccag cgtgacattt ttggtttccg gaatgagcca gaaagtgatg
    33841 cccacaaacg caatgttcag cgcagtgtag agccagaacg taccggcagc gccaatgcta
    33901 tcaagcagtg tcaggaaggt cgcgccgata atcatattcg acacccagtt tgtcgtcgtc
    33961 gaacaggtaa taccgaaatc gcggcatttc agcggctgaa tttcagagca caggatccac
    34021 accactggcg cggcgctcat cgcataaccg gcaatacaca tcatcgtcat gccaacagag
    34081 agccaggaca agccactgga agccgtaccg ttatcaaact gcatcaggca atagcccagc
    34141 accagagtgc ctaacgccat cacgctgaaa ccaattttca gagccggttt acgacctgct
    34201 ttatctaccg taaagaccgc aatgaaggtc gcgaacataa aggtcagtcc cacgaccaga
    34261 gtcgcaatca tctgttgttc tgtggtcgta aagcccgcca ttttgaagat acgcggcgca
    34321 tagtacatga tgatgttcat accggtaaac tgctgcatcg cctgcaacaa cataccgaga
    34381 aacacagcac gacggacgtt acggttgatc ttaaacagtg cccaaccgcc ttgttttaac
    34441 ttcaggcttt cacgaatttc gttgagttct tctcgcgctt tttccgacgt atcgcgcagc
    34501 atacgcaaca cttcttccgc ctcaatatga cgcccctttt ccgccagcca gcgcgggcta
    34561 tttggcagga agaccaccag aataatcagc aggactgctg gtaaagcaag aacccccaac
    34621 attgcgcgcc agttaccgct ataactgaac gctgtatcgg ataaaaacgc cagcacgatg
    34681 ccgagtgtga ccatcaactg gtacatactg atcatcttac cgcgaacgtt ttcacttgcc
    34741 atttcagaaa gatacagagg agcggtgtaa gacgcgatcc cgacagcaat gcccagcacc
    34801 acacgagcgg cgattaacat ctctacgctg gtcgcaaaag cggaccctat agaaccgagt
    34861 acaaacagga tggcccccgc catcaggctg tatttacgac ccaggcggaa cgacagccaa
    34921 ccattaaaca gcgcaccaat tgctgcgccg agcatcatgc tactaaccac ccattcctgc
    34981 aaacgactgg tcagcacaaa gtgatcggta atgaacggca acgctccggc gattacgccg
    35041 atatcaagac caaataacaa tcctgcgacc gcagcagcta ccgaaacaaa catattcata
    35101 cgccgcgtat cccgcaaaga acgtggcgtt aaagcagatt ccgtattgat agtaaccatt
    35161 ttttcctgcc agcagagagt aagacatagt gaaaaaatac gtgaacaact cacgcaggtg
    35221 tcaggtcgga aacagcataa atatggatta aattgctgcg acatgtcgtt atgtgatgga
    35281 tattccaatt ttcaa
//
LOCUS       DAZGLJ010000025        35122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000025 DAZGLJ010000000
VERSION     DAZGLJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.025"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..232
                     /locus_tag="ACHK49_001125"
     CDS             <1..232
                     /locus_tag="ACHK49_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFO9261811.1"
                     /translation="QKISLLSFQKYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRF
                     FRIWFFLQRHKDFAFKPFFTNFNTIERIENYL"
     gene            229..721
                     /locus_tag="ACHK49_001126"
                     /pseudo
     CDS             229..721
                     /locus_tag="ACHK49_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
     gene            complement(763..1713)
                     /gene="corA"
                     /locus_tag="ACHK49_001127"
     CDS             complement(763..1713)
                     /gene="corA"
                     /locus_tag="ACHK49_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO9261812.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1865..1921)
                     /gene="ysgD"
                     /locus_tag="ACHK49_001128"
     CDS             complement(1865..1921)
                     /gene="ysgD"
                     /locus_tag="ACHK49_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO9261813.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2083..2847
                     /gene="yigE"
                     /locus_tag="ACHK49_001129"
     CDS             2083..2847
                     /gene="yigE"
                     /locus_tag="ACHK49_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO9261814.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(2994..5156)
                     /gene="uvrD"
                     /locus_tag="ACHK49_001130"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(2994..5156)
                     /gene="uvrD"
                     /locus_tag="ACHK49_001130"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO9261815.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(5240..5956)
                     /gene="yigB"
                     /locus_tag="ACHK49_001131"
     CDS             complement(5240..5956)
                     /gene="yigB"
                     /locus_tag="ACHK49_001131"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO9261816.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREVEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(5956..6852)
                     /gene="xerC"
                     /locus_tag="ACHK49_001132"
     CDS             complement(5956..6852)
                     /gene="xerC"
                     /locus_tag="ACHK49_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO9261817.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(6849..7556)
                     /gene="yigA"
                     /locus_tag="ACHK49_001133"
     CDS             complement(6849..7556)
                     /gene="yigA"
                     /locus_tag="ACHK49_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFO9261818.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(7553..8377)
                     /gene="dapF"
                     /locus_tag="ACHK49_001134"
     CDS             complement(7553..8377)
                     /gene="dapF"
                     /locus_tag="ACHK49_001134"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO9261819.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(8414..8617)
                     /gene="yifL"
                     /locus_tag="ACHK49_001135"
     CDS             complement(8414..8617)
                     /gene="yifL"
                     /locus_tag="ACHK49_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO9261820.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8967..9521
                     /locus_tag="ACHK49_001136"
     CDS             8967..9521
                     /locus_tag="ACHK49_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO9261821.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            9859..11367
                     /locus_tag="ACHK49_001137"
     CDS             9859..11367
                     /locus_tag="ACHK49_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO9261822.1"
                     /translation="MIGTSRQDGFMHYRNVVCLLSCSLFLSSAWGCRLDPPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKR
                     TDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPDSFRVLAQK
                     SWDQRGKFSTAFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLL
                     TWRDKNGLQRKVLDKENLEWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQ
                     FQPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAE
                     GWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLW
                     RYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            11364..12689
                     /locus_tag="ACHK49_001138"
     CDS             11364..12689
                     /locus_tag="ACHK49_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO9261823.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTSDRQWSKWIDLPDIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDRSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTHVREENVRELHNRDIIIPLTDDSCRNAATDGQTLKP"
     gene            12737..13057
                     /gene="cyaY"
                     /locus_tag="ACHK49_001139"
     CDS             12737..13057
                     /gene="cyaY"
                     /locus_tag="ACHK49_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO9261824.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(13097..15643)
                     /gene="cyaA"
                     /locus_tag="ACHK49_001140"
     CDS             complement(13097..15643)
                     /gene="cyaA"
                     /locus_tag="ACHK49_001140"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO9261825.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDNDTPLLQQYFS"
     gene            16030..16971
                     /gene="hemC"
                     /locus_tag="ACHK49_001141"
     CDS             16030..16971
                     /gene="hemC"
                     /locus_tag="ACHK49_001141"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO9261826.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            16968..17708
                     /gene="hemD"
                     /locus_tag="ACHK49_001142"
     CDS             16968..17708
                     /gene="hemD"
                     /locus_tag="ACHK49_001142"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO9261827.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            17730..18935
                     /gene="hemX"
                     /locus_tag="ACHK49_001143"
     CDS             17730..18935
                     /gene="hemX"
                     /locus_tag="ACHK49_001143"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO9261828.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            18938..20134
                     /gene="hemY"
                     /locus_tag="ACHK49_001144"
     CDS             18938..20134
                     /gene="hemY"
                     /locus_tag="ACHK49_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO9261829.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            20813..20950
                     /locus_tag="ACHK49_001145"
                     /pseudo
     CDS             20813..20950
                     /locus_tag="ACHK49_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(21058..22293)
                     /gene="aslB"
                     /locus_tag="ACHK49_001146"
     CDS             complement(21058..22293)
                     /gene="aslB"
                     /locus_tag="ACHK49_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO9261830.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(22440..22516)
                     /locus_tag="ACHK49_001147"
     tRNA            complement(22440..22516)
                     /locus_tag="ACHK49_001147"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22480..22482),aa:Pro,seq:tgg)
     gene            complement(22559..22645)
                     /locus_tag="ACHK49_001148"
     tRNA            complement(22559..22645)
                     /locus_tag="ACHK49_001148"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22609..22611),aa:Leu,seq:cag)
     gene            complement(22666..22741)
                     /locus_tag="ACHK49_001149"
     tRNA            complement(22666..22741)
                     /locus_tag="ACHK49_001149"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22706..22708),aa:His,seq:gtg)
     gene            complement(22800..22876)
                     /locus_tag="ACHK49_001150"
     tRNA            complement(22800..22876)
                     /locus_tag="ACHK49_001150"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22840..22842),aa:Arg,seq:ccg)
     gene            complement(22979..24364)
                     /gene="yifK"
                     /locus_tag="ACHK49_001151"
     CDS             complement(22979..24364)
                     /gene="yifK"
                     /locus_tag="ACHK49_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9261831.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(24555..25295)
                     /gene="wecG"
                     /locus_tag="ACHK49_001152"
     CDS             complement(24555..25295)
                     /gene="wecG"
                     /locus_tag="ACHK49_001152"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO9261832.1"
                     /translation="MNNNTMAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(25298..26650)
                     /gene="wzyE"
                     /locus_tag="ACHK49_001153"
     CDS             complement(25298..26650)
                     /gene="wzyE"
                     /locus_tag="ACHK49_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO9261833.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(26647..27726)
                     /gene="rffT"
                     /locus_tag="ACHK49_001154"
                     /gene_synonym="wecF"
     CDS             complement(26647..27726)
                     /gene="rffT"
                     /locus_tag="ACHK49_001154"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO9261834.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDLSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEGA"
     gene            complement(27723..28973)
                     /gene="wzxE"
                     /locus_tag="ACHK49_001155"
     CDS             complement(27723..28973)
                     /gene="wzxE"
                     /locus_tag="ACHK49_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO9261835.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPATIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(28975..30105)
                     /gene="rffA"
                     /locus_tag="ACHK49_001156"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(28975..30105)
                     /gene="rffA"
                     /locus_tag="ACHK49_001156"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO9261836.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(30110..30784)
                     /gene="rffC"
                     /locus_tag="ACHK49_001157"
                     /gene_synonym="wecD"
     CDS             complement(30110..30784)
                     /gene="rffC"
                     /locus_tag="ACHK49_001157"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO9261837.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(30762..31643)
                     /gene="rfbA"
                     /locus_tag="ACHK49_001158"
     CDS             complement(30762..31643)
                     /gene="rfbA"
                     /locus_tag="ACHK49_001158"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO9261838.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(31662..32729)
                     /gene="rffG"
                     /locus_tag="ACHK49_001159"
     CDS             complement(31662..32729)
                     /gene="rffG"
                     /locus_tag="ACHK49_001159"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO9261839.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(32729..33991)
                     /gene="wecC"
                     /locus_tag="ACHK49_001160"
     CDS             complement(32729..33991)
                     /gene="wecC"
                     /locus_tag="ACHK49_001160"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO9261840.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(33988..35118)
                     /gene="wecB"
                     /locus_tag="ACHK49_001161"
     CDS             complement(33988..35118)
                     /gene="wecB"
                     /locus_tag="ACHK49_001161"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO9261841.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
BASE COUNT         8844 a         9431 c         8813 g         8034 t
ORIGIN      
        1 tcaaaaaata tctcttttaa gttttcaaaa atacgctata ggatggagtg caacaattca
       61 ttttgtagca caggaccact tcggtcttga tgtaactgat atcaaaaaca agacttacag
      121 caaatataga ttttttcgca tatggttctt tttacaacgg cataaagact ttgctttcaa
      181 accttttttc accaatttta acactatcga aagaatcgaa aattatctat gaaaagcaaa
      241 ctccagaaaa ttatattatg tttatttctt ctatgctgca tctataattt atggacattg
      301 cgtcctgtac agattttgta tacatactct gatgctggca attcagtctt tctggtcgtt
      361 gatcatctgc catggactga cagcgataaa ataaattggt atctgaagca tcagaatgaa
      421 attaaaaatc aacatcccct accagaaggt agttggcata cctggtatgt cattgatatc
      481 ggtaatggct tcactgacta taaaaaaata tatcgaaggg ccgtatgaag atctctactg
      541 tttcccgaca attaagagca atgataattg catcgtgaaa aattacttaa tggttattaa
      601 tgaataccct tatcgtaaca ctcatattgg tattaacgat tttactgagt atcaattaac
      661 acaagaaaat aaaatagagc gagttttcaa tccgcatgac tttaaaaaag acaattttta
      721 gaaataagcc ggataaaaaa atagctttat ccggcactca tattacaacc agttcttccg
      781 cttaaagtac agatacggtg ccaggcccgc gaggatcata aagataatcg cgcctgggta
      841 gccgaaactc cacttcaact ctggcataaa ttcaaagttc atcccgtagc tggaagccac
      901 cagcgtcggt ggcaggaaca ccacggatac caccgagaag attttgatga tgcggttctg
      961 ctcgatgttg ataaagccca tcgccgcctg catcaggaag ttcaccttct ggaacaggga
     1021 ttcgttatgc ggcagcaggg attcgatatc tcgcaggatt tcacgcgcct gctccagttg
     1081 cccacccggt aaacgcgcct tgcgtaccag gaagttgagc gcgcgctggg tatccatcag
     1141 acacaggcga actttccagc cgatatcttc cagttccgcc agcgtggaga gcgcctcgtc
     1201 gtactcatcg ccctgatgcc cttccataat cacccggctc aactgctcca ggtcgctata
     1261 gatattttca atttcatctg ccaactgttc gattttggtt tcgaacagat ccagcagcaa
     1321 ctcgtaggcg ttaccgtcta ccatcgactg gctacgggca cgcatacgat acagacgaaa
     1381 agcgggcagt tcacgctcac gcagagtaaa cagacgacca tcacggatgg taaatgccac
     1441 agtggagtta ccggcgtgat cttccgcatc ttcaaagaag aagaaggagt gaatatgcag
     1501 gccgtcgtcg tcttcaaaga aacgtgccga tgcttcgatg tcttccagtt ccgggcgggt
     1561 tgccaggctc tggccaagtt cagattgtac gcgcagtcgc tcgtcgtcgt ccggttcgac
     1621 aagatcaatc catactgcat ttacaagggg ttgtgactct tcgacttcca gccgggtcag
     1681 tcggttattt tccagttgaa atgcgctcag catgaccggg actcccaatg cttaaaatat
     1741 cggacagttc ggtgggcaca cagaaacaaa ttgggtttca gaccattaaa cagcctgact
     1801 cagcgcgacg ggaaaaattg aggtcgctga caaccgctaa ggctatcagc aaaaagggat
     1861 agccttagga gttgatcctg gatgacagga tagtgagcca gtatctactg ggtgtgtcca
     1921 aggcgaatgt cctcttagag tgatcgtgcg cgcatgttac gccagcaaaa ttttgccgtc
     1981 aacacgcaac agaacaccac agaacaatag ttgaacttcg cgctttttgg tactcgtcaa
     2041 ggtcgctaac aggataaaaa aatccaacat ccaggaccta taatggcgca tcggctactc
     2101 attggtaaag gaatgatcac cttgaatctc aaacggattt ttctcaccct caccttactt
     2161 cccttgtttg ctgttgccgc tgatgattgc gcactctcag atccgacgct gaccgtacag
     2221 gcgtataccg ttaatcctca aacagagcgg gtgaaaatgt actggcaaaa agccaatggc
     2281 gaagcgtggg gaactttaca tgctctgctg gcggatatga atagtcaggg tcaggtgcag
     2341 atggcgatga acggcggcat ctatgatgaa agctatgcgc cgctcggttt gtacatcgaa
     2401 aacggtcagc agaaggtggc gttaaatctc gcttcaggtg aagggaattt ctttatccgt
     2461 cctggcggcg tgttttatgt cgcgggagat aaagtcggca tcgttcgtct ggatgccttc
     2521 aaaaccagta aagagattca gtttgcggtg cagtcagggc caatgttgat ggaaaacagt
     2581 gtaattaatc cgcgtattca tcccaacgtc gcctcacgta aaattcgtaa cggtgttggg
     2641 attaataaac atgggaatgc cgtgtttttg ttgagccagc aggcgacaaa tttttatgat
     2701 tttgcctgtt atgccaaagc gaaactgaac gttgagcagc tgctttatct tgatggcacg
     2761 atttcacata tgtatatgaa aggcggggca atcccgtggc aacgctatcc ttttgtcacc
     2821 atgatttccg tagagcggaa aggttaaaac gatagttttg tgtcagatac tgaagatggc
     2881 gcagatgccg gatgcggcgt aaacgcccta tccggcctac atgacgttgc aatttattga
     2941 atctgaatga ttttttaggc caagtaaggt gcgcagcacc gcatccggca acgttacacc
     3001 gactccagcc gggcataagc cgccaccagc catttaatac cctggccctg aaatgccacc
     3061 tgcaaacggc tatgctcacc gctgccttcc atattgacaa tggtgccttc accaaactta
     3121 gcgtggcgta cgcgctggcc gagcttgtag ccgctgtcgt tctcgaccat cggcgtaccc
     3181 atccgctgat ggctgaccgg gcggcttacc gtggcgcgca ggcgcacctc ttccacacac
     3241 tcttccggca gctcgccaat aaagcgcgac gggcgatggt aaacctcttt gccatacaga
     3301 cggcgagttt ccgcgtaggt cagcgtcagt ttctgcatcg cgcgggttac gccaacgtag
     3361 gccagacggc gttcttcttc cagacgcccg ccttcatcca gcgacatctg gcttgggaac
     3421 atgccctctt ccataccgac gataaacacc tgcgggaact ccagaccttt cgccgagtgc
     3481 atggtcatca gttgtaccgc atcttgccag gtatccgcct gcccttcgcc tgcttccagc
     3541 gccgcatggg ataggaacgc ctgcagcggc attaaatctt cgtcttcttc gttgtagctg
     3601 aactggcgcg ttgccgtcac cagttcctct aagttttcga tacgcgtctg acctttttcg
     3661 cccttctcct gctcgtacat ggtacgcagg ccggagtctt taattacccg gtcagtctgt
     3721 acatgcagcg gcatatcggc agtttcctgc gctaaggcgt cgatcagttc cataaaccgc
     3781 tgtaaggcgc tggcagcacg tccggcgagg gctttttcct gcaacagttc acgacatgcc
     3841 tgccagagtg ttaactggcg atcgcgcgat gtctgacgta ccacgtccag cgtccggtca
     3901 ccaatacccc gcgttggcgt attcaccaca cgctcaaagg ccgcgtcgtc gttacggttg
     3961 gcaatcaggc gcagatacga gagcgcatct ttgatttcct ggcgttcgaa gaagcgcatc
     4021 ccgccgtaaa tacggtacgg catactcgcc tgcaacagtg cttcttccag cacacgcgac
     4081 tgggcgttac tgcggtagag aatggcgcac tcggcaagcg ccccgccgtt gtcctgccag
     4141 gttttgatgc ggttaaccac aaaacgcgct tcatcgagtt cgttaaaagc gcaatagagg
     4201 gaaataggct caccgtccgc gccatcggtc cacagttttt tacccaaacg cccgttgttg
     4261 ttttcaatca gggcgttcgc ggcgctcaga atattgctgg tagagcggta gttctgctcc
     4321 agacggatag tttcagcacc ggggaaatca ttgaggaaac gctgaatatt ctccacctgc
     4381 gctccacgcc agccgtagat tgactgatcg tcatcgccaa cgatcatcac tttgccggta
     4441 tcgcccgcca gcaggcggat ccacgcatac tgaatgttgt tggtatcctg gaattcgtcc
     4501 accaggatat tggtaaaccg ttcgcgatag tgctgaagga tatgcggctt gttaagccac
     4561 aactcgtgag cacgtagcag cagctcggcg aagtccacca ggcccgcgcg gtcacacgct
     4621 tcctgatacg cctgatacac cttctgccag gtctgctcca ccggattacc gtagctttga
     4681 atatgatgtg gacgcaggcc ttcatctttc tggctgttga tgtaccacat tgcctggcgc
     4741 ggcggccact gcttctcgtc gaggttcatc gccttgatta ggcgtttgag caggcgtagc
     4801 tggtcctcgc tgtcgaggat ctgaaaatcc tgcggcagat tggcgtccat atggtgcgca
     4861 cgcagcagac ggtgcgccag cccgtggaag gtgccgaccc acatgccgcc ctggctggtg
     4921 cccatcagct gcccgatacg atgacgcatc tccgccgccg ctttgttggt aaacgtcacc
     4981 gccataatcg agtatggcga gcagttttcc acgctcatca gccaggcgat acgatgcacc
     5041 agtacgcgcg tcttaccact gcccgcgccc gccagcacca gaaggttgct gcgtggcgcg
     5101 gccaccgctt cgcgctgttt gtcattaagg ctgtcgagca ggtaagaaac gtccattggc
     5161 accgccgcgt aaaaataggt tgggcggaga agcgcaacgc cctccgccaa aaagctgggt
     5221 atatatacag atttgctgat tatatcagcg aggtcagaga tgccaaccgg gaaatttcca
     5281 gatgcggcag taaacggctg tcccaggttt gcatcagatc gccattttcc ggtctgatcc
     5341 aacaggcctg cattccgctg cgaattgccc cacccacgtc agtggtgaga tcgtccccaa
     5401 catgtaagat ctcgccgatc ggcacgttga gtttttccgc agccagaaag tacatatcgc
     5461 tgaacggttt tgagcgcccg tgcgggccag cgcgcagcac aaactcaaaa taatccccca
     5521 aaccaaacag ttccggctgg gcgttaccgt tggtgatcgc caccagcggc catttcttcg
     5581 ccagctgttt taaggtgtcg tgagtttgct gcgggacgtc gattcggctg cgccatttgg
     5641 caaagttgat cattgctgcg tgtgcgcctg cactggcttc ttcggcactc agcccggcgt
     5701 cgagcatcgc ttgttcaatc gaacgaaaac gccagcgcgt cacgtcgtga taaatctcgg
     5761 gttccacttc ccgtaccgcc tggcgcaggc gttgcagatc ttcattctgg aagctgcgca
     5821 gcgccggatg ataattttgc acaaaggtaa gcgcctctcg ctcggtgcgc aaaatcaccg
     5881 gacggttatc gtaaagggta tcatccaggt caaaggtgag cgccgagatg cgccccaaag
     5941 gccggtaaaa acgcattatt tcccccgttt ggcgcgtgga tgcgccgcat cgtacaccga
     6001 ggcaaggtgt tgaaaatcaa gatgagtata gatttgcgtg gtggagaggt tggcatgacc
     6061 cagtagctcc tgcacaccac gaagatcgcc gctcgactcc agcatatgcg tagcgaacga
     6121 gtgacgtaat ttatgtgggt gaacgtgatt attcagccct tgttttatgc cccattcggc
     6181 aaagcgtttc tgcacattac gcgcggagat acgcttgccc agtttcgaca gaaaaagcgc
     6241 gtcgtcttcg ctaccaaaca ggtcgcgcaa atcaagccag tgctcaatcc acgccacagc
     6301 gttgcgacca atcggcaggc ggcgctcttt gctgcctttc cccataaccc acacttcacc
     6361 agactccagg tcgaggtgtt taatatccag ccccaccagc tcagaaagac gcagacccgc
     6421 gccgtacatc acttccagca ttgcacggtc gcgtacagcg aggggatcgt tgatatcaat
     6481 atccagcagc cgattcatat cgtcgacgtc gatgtttttc ggcagatgac gcggcgcttt
     6541 cggtgccgaa acacctttcg ccgggttagc tttgagttcg ttctggctga ccagccagtc
     6601 aaaaaagcta cgtagcgcag aaagccgtaa cgccagactt gctgctccca gccctttacg
     6661 gcgactgcgt acagcaaaat tgcgcaccat cgtcacatca cattgctgcc agctttgcag
     6721 gccgttttcg ctggcaaaat tgatgatcgc ctcaagctga cgctggtagt taagcagggt
     6781 tatcgggcta agctggcgct ccacgctcag ataacgtagg tagcgttcta catcggtgtg
     6841 taaatcggtc atacgcgttc aatccaacgc tccagaagct ccggcaacat cagcgcaatt
     6901 tcatgaagta actgcgttcc ttgcccttgt tgatagtgac tggcatcgcg actggtaaac
     6961 agcacgacac ccaaatcagc atcgcttccc agcatcgaca tcgccaccga tcccaccgct
     7021 ttcgcttccg gtagcaccac cagcagctct ggtccgttaa gcggcccaag atagtgctgt
     7081 tcctgcccca aacgctgaat acgcagcggt tcgaaagact gacggcttaa tgccagatga
     7141 gtgtggttcg acggcgcacc taagcgccag cgatccggaa acaggcgcag actcgcacct
     7201 gccaggccga gatcgcgcgc ccagcggtga aagcgcatca gcatatcgtc gagactgctg
     7261 gcggcggtga gactgcgctg caggtagagt aggcgataaa acaggccttc gttggcgata
     7321 gcctgttcca tcaacagcgc catgttctct tccagaacat gaatatgatt acgtgcgcgg
     7381 gccatgtgcc actcgaccaa cgaaacggtg ccgcgtaccg gatgcggcac acgtatcgct
     7441 tctactgcgc gcgcattacg gataaaaaac tcaggatttt taatcagata atcgacaacc
     7501 gcccggtcat caagctccgt gagtgtttcc tgcagttctt cccctggttg cttcatagat
     7561 gaataaatcc gtcgtagaca tgtaccgccg ggccagtcat atataacggg tgacccggac
     7621 ctttccaggc gatatcaaga cgaccgccgg ggagttccac gcgtacttct tcggccagca
     7681 aaccttgctg aatccctact gcaaccgccg cacacgcgcc gctgccgcag gcctgggttt
     7741 ctcctgcccc acgctcataa acgcgtaaac gaatatgctc gcgcttaacc acttgcataa
     7801 aaccgatatt ggcgcgctcc ggaaaacgct cgtggctttc cagaacagga ccaagcgttt
     7861 ctaccgccgc ggtatcgaca tcatcgacct gaatcacgca atgcggattt cccatcgaca
     7921 ccacgccgca taagattgtc tgctcggcgg cgcgcataat ataggtcttt tccgctttgt
     7981 tagcgcgaaa cggcacggcg gaaggttcga agttgggttc gcccatattt acgcggacca
     8041 gatcatcatc ggtgacggtc agaaccatcc gcccgttggc ggtgctgacg cggatatcac
     8101 gcttattggt cagtcctttc agacgcacaa aacgggcaaa gcagcgcgca ccgttgccgc
     8161 actgcgccac ttcactgcca tcagcattga aaatgcgata gtgaaaatcc agttcaggat
     8221 catacggcgg ctcaaccacc agcagttggt caaaccctac ccccaggtgc cgatcagcca
     8281 ggcgacgaat cagctccggt gaaaaaaaga cattctgcgt taccgcgtcg acgaccataa
     8341 aatcgttgcc aaggccatgc attttcgaga actgcatcat ttactccaat cacgcgggta
     8401 cagaaactga cttttagtaa ttcacctggg atggaccatc gccagtggcg cgatcgtttt
     8461 tatccggcac cgtggattgc gtttgcgtct ctaccggttt ggtcggcggc ggtgcgtttt
     8521 tatctgcagg cgggaaatag agcggacctt tcagaccgca gcccgtcagg ctgaagagag
     8581 taattaatac agtgagtgcc ttaaacacgt ttttcattat ggattgcctg taaagttcat
     8641 cgctttctgc tttctatcat cgcaggagaa gacgtaaaag caagcgttta gcgactcttt
     8701 atgaagtctg aaaagtgagg aaatgctgcg agtataacgc ttactcgtgg ggatttatgt
     8761 tttgctggta gtttcgtgat cggcacctcg cgccaggatg gctctatgca ctatcgcaat
     8821 gtagtttgtt tgttggcttg ttcgttgttc ttatcgtcgg catggagctt ttaaaagagt
     8881 ggcgcaccat gcaattttgt tgtctgacct tagtcgccag tgacatacaa ctttgccaac
     8941 ctggtgataa gagggaataa aatctgatgg ggctatttac ccaactggaa aaatttgacc
     9001 aaaagcttac gcgcggttat gcccggtggg ggcgctgggt atggcggacg ttaattgtcg
     9061 ttccggtact ggtcgtgttg tggaatatag gacaggcagt atggggagga ccaagaggtg
     9121 gagtgattct ggaaatccac agtgaaattg accgcccgat actgggattc agtgttaacg
     9181 gcgtggcggg cgcgaatgct tttgcaaatg gtggcggttc aacaacctgt tgtggtgatg
     9241 ttagtgggga tacggcagag gttatctgga cattaagtac tactcgtaca cagtataatg
     9301 ctggaatgcg tctggagaaa cggaatatga cgttacctct ccccaaaaga gaatggggag
     9361 aagattttct gcatgtgcat tttatgcctg gcgataaagt gctgctgggg tggagtaaag
     9421 attctttttc tccgtatgaa gatcttcaca acggggggta caagacccgc gttcggcagg
     9481 atgtgaaaga taaactttat ggtacaggga aaatgaattg atagatttct ataaaaatca
     9541 cgttcggcag aaagcgtaat aatgtgggga atgttagata ccggtggtgg gacctaagta
     9601 caacacgcga aagacaataa agtaggtaac ggtgggcaga gtcgcatgtt gacacccttc
     9661 ccggcgtcaa tccggcacat tttaacgtga tcagtcacac catggcgcag tagctcgtat
     9721 ttatatcgtg aatgaaaacc tcaagtttac cccagcaact atcagaaaat agcgatggcc
     9781 tttatagatt ctgaaaagtg acgatttgcc gcaaaagtta cgcttacgtg cgaggaatta
     9841 tgttctgctg gtagtttcgt gatcggcacc tcgcgccagg atggctttat gcactatcgc
     9901 aatgtggttt gtttgttgtc ttgttcattg tttttatcgt cggcatgggg atgtcggctt
     9961 gatccgcctg aacataatat ttaccagaaa cagggaaaag gcgtggtata cctgcgccct
    10021 tataaagaaa caaatctttc tcttcctgac gtgaattaca aaagtttgcg ccgtttaccc
    10081 aatttattaa tagacccgac aacactagac aaatgggata aagtacctcc tctgactgat
    10141 ctgacaaccg actacctcta tgaaggcgca caggcctggt atcctcatta ctcctggcat
    10201 agcgatggtc gctatatcct ctacgcgggt gaagttgtgc aaaacccgcc agacaaacct
    10261 cccgtcgatg tcgcctcgtt caaggcgtgg ggtgattttg ccgcagataa acacagtctc
    10321 tatttcgaag gcaagcgcac cgataacaat ggcgggggaa atcgcttaga tatcaaaaca
    10381 ctgcatcagg tggaattccg gcctccctgg gatcctgact tactggggct gatattacgt
    10441 gacgctaatt tcttgtatat caatgggcat cgcctcgcag atcccgatag tttccgtgtg
    10501 ctggcacaaa agtcatggga tcagcgagga aagttttcta ccgcgtttaa tccctgtgtg
    10561 gctatgccat ttggcccctg ggataccctg gctcgtacac ggaccaaaat cctgatcaac
    10621 ggcgaacagc ttgatgccga cccggacacc ttttccgtcg tgcgctggat gcccggctca
    10681 ctcttgacct ggcgtgataa aaacgggctg cagcgtaaag tcctcgacaa ggaaaatctg
    10741 gagtgggatg aagatttaac aaagcactgt ctggattttt ctctgctgga aaagaaagtg
    10801 ttctggcgta aaggacctgc ttgtaaacag gaagaattac ccggactcga tccggagcag
    10861 tttcaaccca tcagtgatgc tgtcgcccag tatcaggact cgctttatac catcatcgaa
    10921 acagagtctg gtgaccgcaa gctggaaatc gtgaaacttg atgatcccaa tcttattatc
    10981 aacaaacgtt tcaacgccgg gaaacgccac ggctatttac ttacgcgtgc cgaagggtgg
    11041 ccataccatt ccggtttaca tgtgtttgaa tctgacggac cgctgatctt actggataac
    11101 cgttctccgg atgaacgcga agcccatctt aatgaccatc cctttttgcg cagatggtat
    11161 gcccgcgata accgctacat ttacagcttt gatggcgcgc agctctggcg ataccgcacc
    11221 gctgatccga aacaagttcg cttaatctgg aaggaacaac attcgggata tggctatggc
    11281 gtaaattaca aaacgggata tctggacgga aaaattactg atgacggcga atttattcct
    11341 gccccgcgca atgaggcgac aaaatgaagg gtcaaataat cacagttgca ctcatcctgt
    11401 tgggacacac atttctgacg cctgctgtac aggccatagg gttcgactat tacaatgatc
    11461 acggtgttat gtcttacggt aaaggttatt ggggggataa gaaaatcatt gcccagtttc
    11521 cggagatgag cagggccgat ttgcgccttg tgacaaatat atccggggag cgggagttgg
    11581 tggaaggcta tatacccacc gatgaaataa gcataaaaaa tgaggaatac cactgggtga
    11641 cagacggccg cgttattctc tggcgtggca aaatcgttag caatccaccg ggaacaccca
    11701 ctgtcgatat tgccagcttt caggctatgg gccgcttcgc ggtcgataaa tatagcctct
    11761 attttgacgg acagcgcact gaaagtaata gcggcgcgtc acgtgttgat ctggcgacac
    11821 taaaagctat cgaaggtaac tctaccacgc tggtggatag taaaaatctc tatttgtccg
    11881 gtcgacgtca ggggagcagc agtaatgtta ctgtattaga aaaaagatgg tggggtatta
    11941 atccacgtct tatgagtgta aacagaaatt tgtattccaa tgatctgctt atccgcagtg
    12001 ggcagaatat ttatttaaat ggcgttcacc ttacggcgaa tgcagactca tttgagataa
    12061 ttcgctggat acctcattca ctgctggttt ttcgcgacaa taagggtatg catcgttatc
    12121 cctttgggca attgtctggc aaagcgatac ccgtagatga tgacgtctct tttgaagtag
    12181 gggaaagtcg cgttcgctgg cgtaaacagc tcacgtccga ccgtcagtgg agcaagtgga
    12241 tagacctacc agatattgaa cctgaacaat tccatctgat tactggcaat attgcgcaat
    12301 ataaagatcg gctgtatgta acaaaattat cgacatttgg tgaagaccag cttgagataa
    12361 tcccgctgga tacgccagac ctggtcattg atcgctcatt taatagcggc aaacagcatg
    12421 cttattttat ccgccaatta cggtcaaaga gcgtgcaaat tattccagtt aacggtccgc
    12481 taactaaaaa cgatcgcttc gcttatgacg atcgcaatgt ttatacatgg accgatacag
    12541 aggtaaggat tacgccctcc ccctgcccgg cgaaaacaca cgtcagagaa gagaatgtgc
    12601 gtgaacttca caacagagac attattattc cgttgacgga tgactcatgc cggaacgcag
    12661 caacagacgg gcaaactttg aagccctgac cccataccgc tatactgccg ccatcactta
    12721 aagacaggat acaacaatga acgacagtga atttcatcgc ctggctgatc aactctggct
    12781 gaccattgaa gaacgcctgg acgactggga tggcgacagc gatatcgact gcgaaatcaa
    12841 cggcggcgta ctgaccatta cctttgaaaa cggcagcaaa atcattatta accgccagga
    12901 gccgctgcac caggtgtggc tggcaaccaa acagggcggc taccattttg acctaaaagg
    12961 cgatgaatgg atttgcgatc gcagcggcga aaccttctgg gatttgctgg aacaggcggc
    13021 gacacagcaa gcgggtgaaa cagtcagttt ccgctaagat tgcatgccgg ataagcctcg
    13081 ctttccggca cgctcatcac gaaaaatact gctgtaatag cggcgtatcg ttgtcctgat
    13141 tggcgggcgg catgttaccg atagattttg tgcggaacgg aatcacctgt tcacgaccat
    13201 caaccttcac aatctgatag aactgcggca ggttgaagtt gataaagctt gagccgtagg
    13261 tgaagcgatc gtgcgatgac gagtagaagc gactgacgtc acgtaccagc tcctctttgc
    13321 tgccttcgca gtggtgatac acctcaaccc ggttgctttc gtcgagaatg tagatattaa
    13381 agccattctc gtcttgcgtt tcttcgaaga agaactggat gatcccttcg ctggcaaagc
    13441 cgtccaccac cgccggtaat ttgacgtgat tggtttcaac ctgcactgac aagccgtgca
    13501 gtttgttatg cgaaatagcg ccataaaact cgatggcgtt ttccagtttt tgtaccgata
    13561 cattcaggcg ttcgaagaac aacccccagg tttgaccaga aacgcgcagc gccttgaaac
    13621 gcccggtttc ctggcgggtg ctggaaaggc gcagttcaat acactcagaa accagttgct
    13681 gcacgcgagt acggattaac ccgcgcagat gctggctata acagaagact tccacgctat
    13741 ctggcggtgc ggcgtcctga tgcattttgc cgagaatagt tttcagggct tcgatcatcg
    13801 attgctcgcc gttgaagtgc agcgtacgca cttcgttcca cgagttgcgg tacagcaggt
    13861 caacgctacc taccaggcaa ttttgattct cgccaaagct gaagacatcc agcttacgga
    13921 aatcaaaatg caccacctga ttgcggaacg ccgctgtcgg gtcatattcc aggttaacga
    13981 taatcgccag atggcggatc tcacacgggc tgtagagcgc cttcggtgtc ggtgcaggta
    14041 agcgcagcgg gaaatggtgc gacacgtcgg cgaccatctc ctgcaactta ggcaaatcga
    14101 caatgccgtt acctttaata tacaaacggg tgcgcgaggt cagcaggccg ttaaaccatg
    14161 cccacgccac cagtttgttc aggtaacggt tatattccag cggctgatgg ctgatgatcg
    14221 actcaatatt tggcgcgcgg ttatacagat accaacctga acggttagca cggcccggcg
    14281 gcacataaat aaaggtcaga ttcggttccg agagatcggg tgaaatctgc gggtttacca
    14341 gcgtcacttt acctggtaat gcttcaaacg cggcatacag cttacgcgtc agcacgccga
    14401 tatcctgcgg actggcggag acgctaaggt tattgcgacg cgcaaagcgg atcagattac
    14461 ggtagctctg catcatcgcg tcgagcaact cgttgtgcgc ctcacgcacc tgatcaatct
    14521 tccagttagc gcggttatcg agcattgcca gacgagcttc gtcccaaccc cactcgctca
    14581 ctaactggct caacactgcg cgacgccagc ctacgcaggc gcgttcacgg ctgagctttt
    14641 cgcacacttt taaatagaag cagcgacgta ctaaatccag acgggtaaaa tcttcaatcg
    14701 ccgtcaggta ttcagtaaca cgctccagca tcatgcagta tggatcgaga ccaaacgata
    14761 caatctcgcc gtcgtgcaaa cgctgtttga tatctttcgc cagcagacgt gggttcgggt
    14821 attcccagga ataggcttcc agcagcagtg ttttcagtac cgctttgtat ggggaatcga
    14881 tactcttgta gagctgccaa aggctggcac caaagtactc ttcagcagaa agcgagctta
    14941 agccaccgag atccagccat tcatttggcg tcagcacgcc ctgcgcgtaa agcgtcatca
    15001 catagtcgtc gtaatgctct tcttcgtcgc acggcaccat attccacaga atacgcttac
    15061 cggcgagacg cacggcggta cgataaaatt cgtcaagcag cagtatatgc tgggtggagc
    15121 cacaatcttc gccccccagg ctgccgcttt cattatgacg gaagcggttt tcatcaatca
    15181 ggaagaagct gacttccaca cccagcgagg cggcccagct ttccagcaga ctacatttac
    15241 gttgtagtaa ctggcgctct tcgctatcga gccaggattg atgacagacc cagatatcca
    15301 ggtcagagga acaactttgc cctacggacg aggtgctgcc catggtgtat acaccagtaa
    15361 ttggaagctc acctttcggt gggtcctgta ctgacattcc acgatacagt tcaagctcgt
    15421 tcaggtagtg gcgttgagtt tcatcaggcg tgtaaaggca aatgcctttg ggaacgttac
    15481 catcaaggta acccggcatt agcggatggt gatagtgcaa caatgtcggc agtagactgt
    15541 agacctgttg gaatgcaggc cccatagcag caagcgcgcg atccacacgc aattgattta
    15601 tggcatccag tctctgtttc agagtctcaa tatagaggta caagacgtat cgcctgattt
    15661 gctacccgtc atgactgtga ttccgccaac atcaacggta acacgcggca ttcgggatat
    15721 ttcgtatgtc aaaggtaacc gttaccactt ttcgcgcctg gtttttttag tttcacgacg
    15781 aaaaaatggt ctaaaacgtg atcaatttaa caccttgctg attgaccgta aagaaagatg
    15841 cgctacatac aagtgtagca ccgtttattc tctgtaaatt ccttattacg acggcgtgaa
    15901 acgcctgtca ggatccactg ccagacctca ttttacggtt tgcgcaggcg tctacgtttc
    15961 accacaacac tgacatcact ctggcaagga tgttaggatg gaccacggat gataatgacg
    16021 gtaacaagca tgttagacaa tgttttaaga attgccacac gccaaagccc acttgcactc
    16081 tggcaggcac actatgtcaa agacaagttg atggcgagcc atccgggcct ggtggttgaa
    16141 ctggtaccga tggtgacgcg cggcgatgtg attcttgata cgccgctggc gaaagtaggc
    16201 ggaaaaggct tatttgtaaa agagctggaa gtcgcgctcc tggagaatcg cgccgatatc
    16261 gccgtacatt caatgaaaga tgtgccggtt gaattcccgc aaggtctggg gttggtcact
    16321 atttgtgagc gtgaagatcc tcgcgatgcc tttgtgtcca ataattatga cagtctggat
    16381 gcgttaccgg caggcagtat cgtcgggacg tccagtttac gtcgccagtg ccaactggct
    16441 gaacgccgcc cggatctgat tatccgctcc ctgcgcggca acgtcggcac tcgcctgagc
    16501 aaactagata acggcgaata cgatgccatc attcttgccg tagccggact aaaacgttta
    16561 ggtctggagt cccgcattcg cgccgcgtta ccacccgaga tttctcttcc ggcggtagga
    16621 caaggtgcag tcggtattga atgccgcctt gatgatacgc gcactcgcga gctgcttgcc
    16681 gcgctgaatc accacgaaac tgcactgcgc gttaccgcag aacgcgccat gaatacccgt
    16741 ctcgaaggtg gatgtcaggt gccaattggt agctacgccg agcttattga tggtgaaatc
    16801 tggctgcgtg cgctggtcgg cgcgccggac ggttcgcaga ttattcgcgg tgaacgccgc
    16861 ggtgcgccgc aaaatgccga acaaatgggg atttcgctgg cagaagagct actgaataac
    16921 ggcgcgcgcg agatcctcgc tgaagtctat aacggagacg ccccggcatg agtatcctgg
    16981 tcacccgccc gtctcccgct ggagaagagt tagtgagccg tctgcgcaca ctggggcagg
    17041 tggcctggca ttttccgctg attgagtttt ctccgggtcg acaattaccg caacttgctg
    17101 atcaactggc tgcgctgggg gagagcgatc tgttgtttgc cctctcgcaa cacgcggttg
    17161 cttttgccca atcacagctg catcagcaag atcgtaaatg gccccgacta cctgattatt
    17221 tcgccattgg acgcaccacc gcactggcgc tacataccgt aagcggacag aagattctct
    17281 acccgcagga tcgggaaatc agcgaagtct tgctacaatt acctgaatta caaaatattg
    17341 cgggcaaacg tgcgctgata ttacgtggca atggcggtcg tgagctaatt ggggataccc
    17401 tgacagcgcg cggtgctgag gtcacttttt gtgaatgtta tcaacgatgc gcaatccatt
    17461 acgatggtgc agaagaagcg atgcgctggc aatcccgcga ggtgacgatg gtcgttgtta
    17521 ccagcggtga aatgttgcag caactctggt cgctgatccc acaatggtat cgtgagcact
    17581 ggttactaca ctgtcgacta ttggtcgtca gtgagcgttt ggcgaaactc gcccgggaac
    17641 tgggctggca agacattaag gtcgccgata acgctgacaa cgatgcgctt ttacgggcat
    17701 tacaataact ctcataacag gaagccataa tgacggaaca agaaaaaacc tccgccgtgg
    17761 ttgaagagac cagggaggcc gtggacacca cgtcacaacc tgtcgcaaca gaaaaaaaga
    17821 gtaagaacaa tactgcattg attctcagcg cggtggctat cgctattgct ctggcggcgg
    17881 gtgtcggttt gtatggctgg ggtaaacaac aggccgtcaa tcagaccgcc accagcgatg
    17941 ccctggctaa ccaactgacg gcattgcaaa aagcccagga gagccaaaaa gccgagctgg
    18001 aaggcattat taagcaacaa gctgcacagc ttaagcaggc gaatcgtcag caagaaacgc
    18061 tggcaaaaca gttggatgaa gtccaacaaa aggtcgccac catttccggc agcgatgcta
    18121 aaacctggct gctggctcag gccgattttc tggtgaaact cgccggacgg aagctgtgga
    18181 gcgatcagga cgtcacgacc gctgcagcgt tgctgaaaag tgcagatgcc agcctggcgg
    18241 atatgaatga cccgagtctg attaccgttc gtcgggcaat taccgatgat atcgccagcc
    18301 tttctgcagt ttcgcaggtg gattatgacg gcatcatcct taagcttaat cagctttcaa
    18361 atcaggtaga taacctgcgt ctggccgata atgacagcga tggttcgccg atggattccg
    18421 acggtgaaga gctttccagt tccatcagcg aatggcgtat caatctgcaa aaaagctggc
    18481 agaactttat ggacaacttc attacgattc gccgtcgtga tgacaccgcc gtaccgctgt
    18541 tagcgccaaa tcaggatatc tatctgcgcg aaaatattcg ctctcgcctg ctggtcgcag
    18601 cacaagctgt accgcgtcac caggaagaga cttatcgcca ggcgctggag aacgtctcca
    18661 cctgggtacg agcttactac gatactgatg atgccaccac caaagcgttc ctcgacgagg
    18721 tggaccagtt aagccagcaa aatatctcga tggatcttcc ggaaaccctg caaagccagg
    18781 cgatgctgga aaaactgatg cagacccgcg tgcgtaacct gctggcacaa ccggcagcgg
    18841 ggacaacgga agctaaacct gcacctgcac ctgcacctgc acctgcacct gcaccgcaag
    18901 gtgatactcc ggcagccgcg ccgcaaggag aataaccatg ctaaaagtgt tattactctt
    18961 tgttttgctg attgcgggga tagtggttgg cccgatgatt gccggtcatc agggttacgt
    19021 gctgatccag actgacaact acaatatcga aaccagcgtc acgggcctgg cgattatttt
    19081 gattctggcg atggtagtgc tgtttgccat tgagtggcta ctgcggcgga tcttccgcac
    19141 aggcgcacac acccgtgggt ggtttgtcgg acgtaagcgt cgccgtgccc gtaagcagac
    19201 cgaacaggcg ctgctgaaac tggcggaagg cgattatcag caagttgaaa agctgatggc
    19261 gaaaaatgcc gatcacgcgg aacaaccggt ggtgaactat ctactggctg ccgaagccgc
    19321 gcaacaacgt ggtgatgaag cacgcgccaa ccaacatctg gaacgcgcag cagagctggc
    19381 cggcaatgac accattccgg tagaaatcac ccgtgtacgt ctgcaactgg cccgtaatga
    19441 aaaccatgct gcacgccacg gcgtggataa gctgctggaa gttacgccac gccatccgga
    19501 agtattacgt ctggcggaac aggcgtatat ccgcacaggt gcatggagtt cgctgctgga
    19561 tatcatccca tcaatggcga aagcccatgt tggtgatgaa gaacatcgtg caatgctgga
    19621 acaacaggca tggattggcc tgatggatca ggcgcgtgcc gataacggta gtgaaggttt
    19681 gcgtaactgg tggaaaaacc aaagccggaa aacgcgtcat caggtagcgt tgcaggtggc
    19741 aatggcggaa catcttattg aatgtgacga tcatgatact gcccagcaaa ttatcatcga
    19801 tggcctgaaa cgccagtacg acgatcgcct actgctgccg attcctcgac tgaaaaccaa
    19861 caatccggaa cagctggaaa aagtgctgcg ccagcagatc aaaaacgtcg gcgatcgccc
    19921 gctgttgtgg agcacactgg gccagtcact gatgaagcac ggagaatggc aggaagcatc
    19981 gctcgccttc cgcgcggcgc tgaaacaacg tccggacgcc tacgattacg catggcttgc
    20041 cgacgcgctg gacagactgc acaagccgga agaagctgca gcgatgcgtc gcgacggttt
    20101 gatgttaacg ttgcagaaca acccaccaca gtagttcctt ctcacccgga ggcaagcacc
    20161 tccgggacct ttctgatgca taaaaaaacg cctgctctta ttacggagca ggcgttaaaa
    20221 caggtctgta tgacaacaag tgggtgcttc actcaacgtt gtgtccatgg tgtctgatga
    20281 ggcataagcg acatctgtca gtggacgata agcaccgtaa acggctctgc gtcattccgg
    20341 agtttatgag gcactaaggc gaacataaga gatggaatga gcatctaccc gtttattatg
    20401 ccacagagaa tcgggaaata acatccctta acacttgtta tgagataatt ctgtaatcct
    20461 ctttgcttcc tgagtaataa cttcctgagt gaatatttaa cctgagcttg atcctacaca
    20521 tacttattat gaatgataaa attcattcaa ttaataacac atatattaat tgccgttaaa
    20581 actaaaaaaa gcatcaataa tcaacgcgat ataataaacc tgccttacat atcaactgcg
    20641 ccagaggtag gattgaaaac gctctcctga ttttccaatt cattttctgg ataaataaat
    20701 aatttatttt tgtcactatt atttatgtaa tcatcctgtc agggagaggg atctcaatta
    20761 tcaatgctta attacgtcat cattttgatg acatgaaacc ggagcatttt gcatggaatt
    20821 ttcgttttca cccaaacttc ttgttgttgc tgtcgccgcc gctcttcctc tcatggccag
    20881 cgcagcagat accccgtcaa ctgccaccgc acgcaaaggc tttgccggat acgatcaccc
    20941 aaaccagtat ccacagaacg ctgcaattta ttgaatttgc acgatcatgt aggccggata
    21001 aggcgtttac gccgcatccg gcaatcaacc gcaggcggcc acaaaatggc cgctttttta
    21061 cttactcacc accagcaaat gcgcatgcat aatgtcactg gccgggcgac cgtgcgccag
    21121 caaatcagcc attgctttaa gatatggcgg tagatggcgg aaataacgct gatacccggc
    21181 acacaaataa ttcagccccg gtttgccgct ggcatcgagc atgaagcggt gtttcgggca
    21241 gcctccccag cacgctttta acacgttaca actgcgacac tgcgccggta attgcttaaa
    21301 tttatcttca ccaaacacct gctgttgcgg ggaatcgatc atttctgcaa ttgtttgctg
    21361 atgcatattc cccagccgat attgcggata aacatagtga tcgcaggcgt aaacgtcgcc
    21421 gttgtgctca acaatcaccg agcgcccaca ggttggctga tgatggcaaa ccgcacccgg
    21481 cgcaccgaca aaattggcaa acgcccattc gatattcatc acgaaaatct tgccgacgtc
    21541 gcgtttgatc cagtggtcga atatcgccac cagaaattca ccaaattcat cggggcgcac
    21601 cgaccattcc gtcagttccc cctgaatatc accaggcgca tgtagtttca gtccatcgct
    21661 ggcagctgtt tcatcagcca ggcgctcgac caccggaata aactggatga attcgactcc
    21721 cgcatcgcac aaaaaatcat aaacctgcaa cggttgctgc gcgctggtgc ggttgacgca
    21781 gaccagcacg ttatagtcga catgatgttt ttgcagcagc gtcagggcac gcatcaccag
    21841 cttatgcgtt ggtctgccac ctttggtcac gcgatattga ttgtggatct cagccggacc
    21901 gtccagcgat aacccaacaa gaaaatgatt ttctgccaga aatgcacacc atttatcatc
    21961 gagcagcacg ccgttagtct ggaagctgtt acttatcttc ctgccagcac cgtatttcgc
    22021 ctgtaacttt acggcacggc ggtaaaaatc cagcccgagt aacgttggtt cgccgccctg
    22081 ccaggtaaaa gccacttcgt tttgcggttc gctggcagcg atatagtgac ggacatacgc
    22141 ttccagcgtg tcatcgtcca tatgcgtgac tggcttttcg cggtaaaggg attgtttttc
    22201 gagataaaaa cagtagtcac agttcagatt acaatcggaa ccactcggtt tcgccatcac
    22261 atgaaaagca cgcgttggaa cctgttgcag catggtcact ccttgcctga agtcatcctt
    22321 caatacgtta agggcgaaat cataccgtgg ggcaatgagt tattacgggc gtttattgcg
    22381 aagggagggt gacgaaatgc acagaaaaca aaaaaccccg ccgaagcggg gttcaaaatt
    22441 ggtcggcgag agaggattcg aacctccgac ccactggtcc caaaccagtt gcgctaccaa
    22501 gctgcgctac tcgccgaaat actgcttttt gaatttttag ttcaattctt taaagtcgtg
    22561 gtgcgagggg ggggacttga acccccacgt ccgtaaggac actaacacct gaagctagcg
    22621 cgtctaccaa ttccgccacc ttcgcattgt cacaacttct aataatgggg tggctaatgg
    22681 gattcgaacc cacgacaact ggaatcacaa tccagggctc taccaactga gctatagcca
    22741 ccactacaaa tcttgttacg cggtattact accaccgcag ctcaagcgcc gggactaaat
    22801 ggcgcgcccg acaggattcg aacctgagac ctctgcctcc ggagggcagc gctctatcca
    22861 gctgagctac gggcgcttag cgccgttgcg ggggtggata atacggactt cccacccctc
    22921 tgtctagtcc ctttttaaat aaattgcgcg tttggttacg gtttgtgcgt tttgctgatt
    22981 attcctccag tttatgcgct ttcccgtggc gattaaggcc aaaaacttta taaatcgccg
    23041 tcaccgccag cataaagatg atgccaacaa acagcgacat acgcgtatct tcattaaagt
    23101 acatgccgat caaaacgcaa atcaggaatg ccattgttac gtagttggcc cacgggaaca
    23161 ggattgagcg gaacggatgg ctggcaatcg ccgctttatg tgcacgccga aaacgcagct
    23221 ggcttatcaa tatcacaaac catggcacca tccccggaag cacgctggca ctgtagacgt
    23281 agacaaacac acgctgcgga ttgggaatga tgtagttcag gcatgagcca attagcagaa
    23341 tagcaataga tactgccaca cccgcaactg gtacgccgtg acgggaaact ttcgccatcg
    23401 ccgccggtaa ctgacggttt ttcgccagtg cgtagagcat acgtccgcaa ctgtacatgc
    23461 cgctgttaca gccagagagc gcagccgtca gcaccacaaa gttgataatg cccgccgctg
    23521 cggtaatacc gattttggca aaagtcagta cgaacgggct gccgttgctg ccaatttcat
    23581 tccacgggaa gatggtgacg ataacgaaaa tcgcgcctac gtagaaaatc aggatccgcc
    23641 acagcacttt gcctacggca ctgcgcagcg tcacctgcgg attcttcgct tcaccggcag
    23701 taatgccaat cagctccacg ccctggtagg acgccaccac aatacacagc gcggtcagga
    23761 accctttcca gccacccgca aagaaaccgc catgctctgt gagattgcta aaaccaatcg
    23821 actgcccgcc attgccaaag ccaaagaaaa tcacgcccag gccaatgaca atcatcacga
    23881 taatcgtggt gactttgatc atcgcgaacc agaactcgat ttcgccgtac aaccgcaccg
    23941 ccgccagatt cgccaacgcc accagcgcca ctgcgatcaa tgcgggtatc cactgcgcca
    24001 tctccgggaa ccagaactgg acataaacgc caatggcggt gatttctgag atccccaccg
    24061 ccatccacat aaaccagtaa gaccaggcgg tgagataacc aaagaacggg ctcatataac
    24121 gatgcgcata aacggcgaac gaaccggtaa ccggttcgag gaacaacatt tcgcccattg
    24181 aacgcatgat gaaaaagacg aatagcccgg cgatgatata ggccaacaat acggatggcc
    24241 cggcccattt caaggtactg gcggccccca taaacaggcc gacgccaatg gtgcccccca
    24301 gggcgatgag ttcgatatgt cgagcttcca gcccacgctg tagctctggt ttgttatctg
    24361 ccataaatcc tcgtgttgtg tttgcatgct ttccggtgtt accggttatc gttatgggta
    24421 catcgagtgt tgcgaatgtt ttcgtaattc aggagaaatg gcaaataaag cattaaaaat
    24481 ttgaatgctt tgtgtaataa aaaagcagac aggcgacgga gtgaccactc cgtcgcttta
    24541 caaagagagg aaaatcagag gttgccggtg tagtgccagc gtaaataacg cagcaaacga
    24601 agctgacgct taatgcggct cggctgcgaa agcaggcggt agagccactc cagtcccagc
    24661 gtttgccaga ttttcggtgc gcgttttacg tgaccggtga aaacatcgta agtcccgcca
    24721 acgcccatat acagcgcatc tggatgcacc agacggcagt cgcgcatgaa gatctcctgc
    24781 tttggcgatc ccatcgcaac ggtgacgatt tgcgctccgc tggcatgaat gcgttcaaac
    24841 agcgcctgac gctgctcggg tttaaaataa ccatcctgac tgccaacgat attcacattc
    24901 cactggttgc gcagtttagc ttcagtttgc gccagcactt caggtttacc gcccacaaga
    24961 aataccggcg tcccttcttt gcctgcgcgc gccatcagct cttcccagag atcagcaccg
    25021 gcaacgcggg aaacctgcgc ctgcgggtac tttttacgta ctgaacgtac aacgctgatg
    25081 ccatccgcat atttaaattc ggcagcgtta attagctccc tgacctcagc attatcttca
    25141 atggtcagca ttttttcggc attaatggca accagcgttc cctgcttaag ctgcccgtca
    25201 gcaaacagat aatcgagggc gtgctgcata tcacgccaac caatcaactg taagccacgc
    25261 agcgtatagg tcggtgccat ggtgttgtta ttcattgtta tccttcaacc tgcgtccgga
    25321 gcgatgattt tgtacgttta tgaatgagtc cggcgctttc aaaaagccag tacaacagtt
    25381 ttgcgatcat cagacatgcg ccgaagacca cgataaaaaa gactacgcgt gagacaaacg
    25441 aatccagccc ttcacgtgcc agcacgatca tattgaagat cgccccaaag cagaaactgt
    25501 gcaatatcgc agccttatag cgattaggct cgcggttgcc cagctcatac agccagtcga
    25561 accatttgat gatcagacca accacgatcg ccccgagcgg gatgaacaac gcgccgccca
    25621 tcaccaccag tgagcctata agcgtaggcg agatcgccag tccggagtgg ttattcagca
    25681 cttcccaggt aaagtagttg gctgagttca gcaccatact cgggcgaccc ggccacagcc
    25741 aggaagggat aaagacatag aaatcgcgga caattggagc caggccctgg aagtcgatgt
    25801 tgtcgtagtt ctgcaacagc aacgccagat tctcccacgg cgagaaggtg tcgcgagtga
    25861 gatagagaaa cgtatagaac gcttcatcgc cgctcacatt cattccatag cgttttagcg
    25921 ccagccagaa catgcccaca atccccagca cgcccgccgc cgccagcatc cacaacgaaa
    25981 tccagccgcg aataatgcca ataaacagga agatagcgaa tgcgatgatg atattggcgc
    26041 gagtgccgcc gacaatcata taagtcagca agccaaaggc gaccgtgctg acgaggaaaa
    26101 acagccacgc tttgctatcc tggcgcagaa agtagaccac cagcatcgcc gggatgaaaa
    26161 agtaaaagaa gcgttttaac gccacgccgg agacttcact ggagaagatc tgactgtagg
    26221 agttaagccg gaacagcaaa aagccgttgt gcatgaagaa gatgccgacg cttaccagcg
    26281 cgatgcccat caggatcacc cacgtaagat tggtctccac gcggttcatg gtaaacagcg
    26341 gacggcgcgg tacatcagca acgcgtttgc gtaggcgggt tttgtaggtg acatagtaaa
    26401 ccgcgtagaa gcagcccgca gaaagcaacg cctgcaacaa gatttctggc ggcgcgacac
    26461 caacatcaaa gcgaaatacc agcacgctgg tcagcgggaa gccgaagaaa aaggtgagca
    26521 aaaacagcaa tgaaaagaag acattgaagt taaagcgcac acggcgaaac tcaaaccagg
    26581 tcagcgtggc aataaacagc gtgcagagca gccagaccac aaacaggcca ctgaattgca
    26641 gcagactcat gcgccctccc cggcggcaat cgccaacgcc cgctgccagc cttgtagata
    26701 gttagggcta aagaaggcaa tggtgttttt atccaccgac gccaactggc gctgcgcttc
    26761 acgcacaata tcctcgttga gatcgtcggt agtaaacagc actggcaaat gctgttccgt
    26821 catatcctgc cagaacggat tttcccggtt aagcacacaa ggaatgcccg cctgaatcag
    26881 taagcacagc gtaccaatgc cctgctggcg agcaaaaata aagtaaccaa gatcgcactg
    26941 acgaagtagc gccagatagg cgtcaaattc cagtttttcg ctcaaaattt gcagattttc
    27001 ttcgctgaat aactccagcc ccgcctgacg aacttcctca atgtacgctt cgttattagg
    27061 cggatatccc atcggcacca ccacttttac cgtatcgcca aattgctgat gaacggcacg
    27121 taaagcggcg acatgctcat tgctgaggtc gccggagttc cccaccagaa tggtcatttt
    27181 tccttcacgt tgccgatcgt tcgccatcgt attgagagaa gggtccatcc gcgtcgggaa
    27241 gtacagcagt tctccccgca cctttgggtg cgttttggca aaaaagctca aatcgccgcg
    27301 ggtggcaaat acacagccga ctcgcttttg cgccaggcga cgtagtgggt aaaaaagctt
    27361 atatctcaag ccactggaaa gctcatacag gtctgccccc cagatatgcc agaaaaactg
    27421 gctgggctta atgccaccac tcagcagagc cagccacagt gtgggattga actgaccgtg
    27481 gaaaaaaaat cgctgctgac ggttagcttt tgctttcgcg atgaccgctt ccgccagcga
    27541 ttttttccca gggaaaaatt gcacagaaag cgccggacag ctatcactta agccgtcgtc
    27601 cttgccaaca accataaact cgcgcgcgtg ctcgctcgtc gcagccagcg cgtcattgaa
    27661 aaaccgcaaa acggttcggt tatggtgagg gatatccgat cccagtacgt gaatcagtac
    27721 agtcatgccc gcctacgcca gagtaaaaac acgccacaac aaagagaaaa atagacgata
    27781 taagttgcca tatatgcctg cgccgcgccc agtgcaccat gcgcagggat tagccagtgg
    27841 gcaaatacca tcaataaagt gaactggctg atttccgcca gaatataaaa ccgcaatgac
    27901 gctttggcga tcaccagata accaaagaca taagcgccca cttttaacac atcacccact
    27961 aactgccagg caaagagatc gcgcatagcg gtaaatttat tcgacaacag cagccagata
    28021 gcaaaatcac gcagcagcca gacggtaaaa ctcgccgccg ccactgccgg taagacgaat
    28081 ttcagcgatt taaccacttc ccgggtgata tcgcgctttt ccgttagccg cgacaacgtg
    28141 ggcagcaaat atacgctgaa cgatgccgta ataaattgca ggtaggcatc ggaaatactg
    28201 ctcacccctt gccagatccc cacttcatcc cagctatact gcgccgccag cagtttacgc
    28261 atcatgatgt aagcaacagg caaggtcacc gacgtaatca acgccatgag cgtaaatttg
    28321 ctcaactgcc ctgccagacc gttatcccag ctgggtttca gatagcttaa cgggatgaca
    28381 ccacgtttga tcaacatgat agtggcagga attaccacca gcgcgggaat cagcgccaga
    28441 cccagcaacg ccccttcata accgcccaaa cggtaactga cgtagtacgc gagaacgcca
    28501 atcaagctgc cgacaatcag cgataacgca ttacctgcgg catcgcgaaa gcctttcatc
    28561 agcgccagta acaggttgcc ccaggcgatc cccatttgca ccagcgccac taaacgcacc
    28621 agcccctgat agtcggtatt accaaacaat ccctggctga ttggcgcagc tgccagcaca
    28681 aaaaccagcg ccatcagcgt agagaaacca agtaccatcg ctgatgaagt gccgaccacg
    28741 cggcgcagct gttgcggatt atcatggtac tgggcaacgt atttggttac accgttaaag
    28801 atgccagctc cggcaagcac gccgagcacg gtaatcaact ggcggaaatt tgccgccagc
    28861 ccaagccccg ccggaccaaa tgacacagcc agcaacttac cgaccagtaa cccggcacca
    28921 atcttgacca gtgtactggc cgccgtccac aaggacgctt ttgccaacga catatcagga
    28981 gaagtagttc aacaaagtcg caattaccgt acgctgattg acgggcgaca ggttgtagaa
    29041 cagcggcagg cgcagcaggc gctcgctctc tttggtggtg tagcgatctt caccgtggaa
    29101 ctcaccaaag cgttcccccg cagggcaacc gtgcagcgga atgtaatgga acaccgccat
    29161 gatttccgct tctttcagaa agttaatcaa cgcgctccgg tcatcaatat cccgcagttt
    29221 aatgtagaac atatgcgcgt tctgcacgca gccatcggga atcgacggca gctcgatacg
    29281 cccggctttc gccagaggcg ctaacgcatc gtagtagttt tgccacagcg ccagacgttg
    29341 ctggttgata cgatccgctg cttccagttg cgcccacagg tacgcagctt gcagatcgga
    29401 catcaaatag ctggagccaa tatcgcgcca ggtatattta tcgacctgac cacggaagaa
    29461 ctggctgcgg ttagtgccct tttcacggat gatctcggct cgttcgatta acgctttatc
    29521 gttaatcagc gtcgcgccgc cttcaccacc cgccgtatag tttttggttt catggaagct
    29581 aaagcagcca atatgaccaa tggttcccag tgcacgccct ttgtaagtgg acatcacgcc
    29641 ctgagcggca tcttctacca caaacagatt atgctttttc gccaacgcca taatggtgtc
    29701 catttcgcag gccacacccg cgtaatggac cggcacgata acgcgcgttt tgtcggtgat
    29761 cgccgcttca atcagcgttt cgtcgatgtt catggtgtcc gggcgaacat ccacaaaaac
    29821 gatttttgcg ccacgcagca caaaggcatt ggcggtggag acaaaggtgt agctcggcat
    29881 gatcacttca tcgccaggct ggatatcgag cagcagcgcc gccatctcca gcgaagcggt
    29941 gcaggacggc gtcagtaaca ctttggcgct gccaaaacgt tgctccagcc actgctggca
    30001 gcgacgggta aaaccgccat cgccacacag tttgccgcta cccattgccg actgcatata
    30061 gtcgagttcg gttcccacca ccggcggtgc gttaaatgga atcatgtgat cacctgtata
    30121 accagtacgc ggtgctttct acattcgcac cactttgtat gtatcgttta agcgcggcgg
    30181 tgttgcccat ttgggtcgcc acccgcaaag ttgttttacc gcgagcatac gcccagttta
    30241 gcgccgtttg catcagctca gcacctacac cgcgtccagc cagcaggcca attcgcgcat
    30301 ctgtcgcatt gagttcccgt aaagagacat aaccgcgaat accgccggac gccgcacgta
    30361 aaatcagaca ttgatgatca aaggtgccgc gcacggcatt ttcaatccac tgtgcataaa
    30421 agcgtctgct ggcgtcaggc gcataccacg gcgcacgaaa acggctttgc gcaaatgcgg
    30481 cgctggctaa ctgacgtaat gcgggaatat cggtctcttg tgccactaca gcaccactat
    30541 cactgacatt gttcacgggt agtgccaaat caacttcacc ttctaccagg gagaatccca
    30601 gctgttgcag ggcatccagt tcacccgtat ttgatgccgc aattttggcc tgcacccgtg
    30661 accacggcgc taacacgtct ggcgtcagga gcggtgcttc agacgtaatg cgcacgatgg
    30721 cgctgttaac accaaagaag gcgttttccc aggttagtgg ctcaatactg gcgcggacgg
    30781 gcacgaagta actccagcag atattggccg tagccagttt tcgctaatga actggcagca
    30841 cgcttcacac cctcgtcatc gagccagccg ttacgccagg cgatctcttc caggcaggca
    30901 atcttaaagc cctggcgttt ttctactgtc tgcacaaagg tactggcttc aatcaggctg
    30961 tcgtgagtgc cagtgtccag ccaggcaaat ccgcgcccga gcagttcaac ggtcaggttg
    31021 cccgcctcga ggtacatctg gttgatggag gtaatctcca gttcaccacg ctccgacggc
    31081 ttcacctgct ttgcgtactc cacgacttta ctgtcgtaga aataaagccc ggtcaccgcc
    31141 cagtttgact tcggctgttt tggcttttct tccagcgaga tagcgcggaa attgtcgtca
    31201 aactccacca cgccaaagcg ttccgggtcc atcacctgat agccaaatac cgtcgcccct
    31261 tccgtgcgcg ccgcaacatg acgcagcttc ggactgaagc cctgaccgaa gaagatgtta
    31321 tcgcccagca ccagacaaga aggttcaccg ttgaggaagg tttcaccgat gataaaggcc
    31381 tgcgccagac cgtccgggct gggctgttcg gcgtattcca gctggatacc gaactcacta
    31441 ccatcgccca gcaggcgctg gaaataacct ttatcttccg gcgtagtgat gatgagaatt
    31501 tcgcggatac cggccaacat cagcaccgac agcggatagt aaatcattgg cttatcgtaa
    31561 atcggcaaca gttgcttcga tacgccgcgc gtaatcggat gcaatcgggt gccggaaccg
    31621 cccgccagga taataccttt catgtttgcc tccgctgaaa attagccttt cagacctaaa
    31681 cgctcgccct gatagctgcc gtcctgcacc tgcttccacc agctttcatt agccagatac
    31741 cactgcaccg ttttacgcat tccactttca aaggtttcct gtggcagcca accaagttca
    31801 cgggcaattt tcgaagcatc aatggcataa cgcagatcat gccccggacg gtcagcgaca
    31861 aaggtgatca agtcacgata gtgcgccacg ccgtgcggct tgttcggagc cagttcttcc
    31921 agcagctcgc aaatggtttc cacaacatcg agattcttac gctcgttgtg accaccaata
    31981 ttataggttt caccgacttt cccggtggtc gccacgcaat acagcgcgcg ggcgtgatct
    32041 tccacataca gccagtcacg gatttgctgc ccgttgccat ataccggcag cggtttaccc
    32101 gccagcgcgt tgaggatcat cagcgggatc agtttttccg gaaagtggta aggaccgtag
    32161 ttattcgagc agttggtgat cagcgtcggc agaccgtagg tccgcagcca ggcgcgcacc
    32221 agatggtcgc tactggcttt tgacgcggaa taagggctgc tcggcgcata cggcgtggtt
    32281 tcggtgaaga aatcatccgt cgagtgcagg tcaccatata cttcgtcggt ggagatatga
    32341 tgaaaacgga acgctgattt tttatcttcc gtcagcgtat tccagtaagc ccgcgccgct
    32401 tcaagcaatg tataagtccc gacaatgttg gtttcaataa acgctgccgg gccgtcaata
    32461 gaacggtcaa catggctttc ggctgccaga tgcatgacac agtctggctg atgctcagtg
    32521 aatacgcgtg ccagttctgc ccgatcgcag atatcaactt tctcaaaggc aaagtgctcg
    32581 ctttgcgcga ccggtgccag cgacatcagg tttccggcgt aggtcagctt atcgaccact
    32641 accaccgcat cgctcgtttc gttgatgata taacgcacca gcgccgagcc aataaacccg
    32701 gcaccacctg ttatcagaat ttttctcatc agcgccagac tcctttggca tcgacgacat
    32761 actgctgatg gacattgtcg ccattgataa ctttgaactg actatgatcg accagcatca
    32821 ccagcacgtc tgccgttgcc agcgcctcgt caagctgcgc cagagtacaa agcccggtca
    32881 gttttttcgg caactggtgg atgttaggct caacaaccag cgtttcgccg ctgtgccact
    32941 gggcgatcag ttcagcgatt tccatcgccg ggctttcgcg caggtcatca atattcggtt
    33001 taaacgccag accaaagcag gcgattttca gttcactggc gcgtttatcg gtagcagcaa
    33061 ggcagtcggc aaccgccgct ttcacctgat cgataaccca gaacggtttg tgatcgttaa
    33121 cttcgcgcgc ggtacggata agccgcgcct gctgggggtt ctgtgccacg ataaaccacg
    33181 gatcaacagc aatgcagtga ccgcccacgc cagggccagg ctgaagaata ttaacgcgag
    33241 ggtgacgatt cgccaggcga atcagttccc agacgttaat cccctgatcg gcacaaatca
    33301 gcgacaattc attagcaaaa gcgatattca catcgcggaa gctgttttcg gtgagcttac
    33361 acatttccgc cgtccgcgag ttagtgacga cacactcacc ttcgaggaaa attttgtaca
    33421 gttcgctggc gcgggtcgaa caaaccggcg tcataccacc aatcacgcga tcgtttttaa
    33481 tcagctcgac cataacctgg cccggtaaca cgcgttccgg gcagtaagca atgttgacgt
    33541 ccgcctgctc gcccacctgc tgtgggaaag tgagatccgg acgcatctct gctaaccatt
    33601 ctgccatctt ctcggttgac cccaccggcg aggtggattc aaggatcacc agcgcgcctt
    33661 ttttcagcac tggcgcaatg gagcgagcag ccgattcaac gtaggtcata tctggctcat
    33721 gatcgccttt aaacggcgtg ggtacagcaa tcagccaggc atccgcttca actggcgtcg
    33781 tgctcgctcg taaaaaaccg ccttctacgg cagtttttac tacgctcgcc aaatcaggct
    33841 cgacgatatg gatttcgcca cgattgatgg tatcaaccgc gtgttggttg atatcgacac
    33901 caatcacctg tttttgccgt gaggcaaacg ctgctgctgt aggcagcccg atgtaaccca
    33961 gtccgataac agaaatggtc gcaaaactca tagtgatatc cgattatttt ttaacgcttc
    34021 cagaatgcga gagcatgcct gaccatcacc atacgggtta tgggcgcggc tcatagtttg
    34081 atattcgttt tcgtctttta aaagacgcgt cacttcctcg acaattcgct gcttatccgt
    34141 gcctaccaga cgcaccgtac ccgccgtcac cgcttccgga cgctcagtgg tatcgcgcat
    34201 caccagcacc ggtttcccca gcgaaggcgc ttcttcctga atgccgcctg agtcggtcaa
    34261 aatcagccag gcgtggttca tcagccagac aaacggtaaa tactcctggg gatcgatcag
    34321 aatgacattt ttcacatgcc ccagaatgcg attgaccggc tctctgacgt tcgggttgag
    34381 atgcaccgga tagacaatct ggatgtcctg gtgcgtggtg gcgatgtctg ccagcgcgtg
    34441 gcagatttct tcaaagccac gaccgaaact ttcacgcctg tgaccggtca ccagaatcat
    34501 ctttttatcg gggtcgataa acgggtaatt tgccgccagt tctgaacgca gcgtgtcgct
    34561 gctcatcacc tgatcacgca cccataacag tgcatcaatg actgtattac cagtaatgaa
    34621 gattcggcta tccgcaacgt tttcacgcag caagttttgc cgggaagttt cggttggaga
    34681 gaagtgatac atcgccagat gcccggtcaa tgtacggtta gcctcttccg gccacggcga
    34741 atagagatcg cccgtgcgta aaccagcctc aacgtgacca acaggaatac gctgataaaa
    34801 cgccgccagg ctggttgcca gcgtcgttgt tgtgtcgccg tgaaccagca cgacgtctgg
    34861 tttgaactcg gcaagaatag gttttagccc ttccagaatc cgacaggtta tctctgtcag
    34921 gccctgtcct ggctgcatta tgttgagatc gtagtcaggt acaatggaaa agagtttcag
    34981 cacctgatcg agcatctccc gatgctgcgc agtgacgcaa actttagcct caaaaaaagg
    35041 atcttttgcc aacgcatgca ccaacggagc catcttgatg gcttccgggc gcgtaccaaa
    35101 tacagtcagt actttcacat cg
//
LOCUS       DAZGLJ010000026        32769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000026 DAZGLJ010000000
VERSION     DAZGLJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.026"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..281)
                     /gene="tccP2"
                     /locus_tag="ACHK49_001162"
     CDS             complement(<1..281)
                     /gene="tccP2"
                     /locus_tag="ACHK49_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
                     /protein_id="HFO9261842.1"
                     /translation="MINSINSFFSSIPHSISSVTRNSSFTASQHKSTPNTVKTSSPLS
                     PSNSPASATTIFKVKNSYTESGLQRSTSYTQSSIEKNALHRPLPDVAKRL"
     gene            complement(727..1131)
                     /locus_tag="ACHK49_001163"
                     /pseudo
     CDS             complement(727..1131)
                     /locus_tag="ACHK49_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspV"
     gene            complement(1214..1301)
                     /locus_tag="ACHK49_001164"
     tRNA            complement(1214..1301)
                     /locus_tag="ACHK49_001164"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1265..1267),aa:Ser,seq:tga)
     gene            1728..2846
                     /gene="hyaA"
                     /locus_tag="ACHK49_001165"
     CDS             1728..2846
                     /gene="hyaA"
                     /locus_tag="ACHK49_001165"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO9261843.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            2843..4636
                     /gene="hyaB"
                     /locus_tag="ACHK49_001166"
     CDS             2843..4636
                     /gene="hyaB"
                     /locus_tag="ACHK49_001166"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO9261844.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            4655..5362
                     /gene="hyaC"
                     /locus_tag="ACHK49_001167"
     CDS             4655..5362
                     /gene="hyaC"
                     /locus_tag="ACHK49_001167"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO9261845.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWMGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            5359..5946
                     /gene="hyaD"
                     /locus_tag="ACHK49_001168"
     CDS             5359..5946
                     /gene="hyaD"
                     /locus_tag="ACHK49_001168"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO9261846.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            5943..6341
                     /gene="hyaE"
                     /locus_tag="ACHK49_001169"
     CDS             5943..6341
                     /gene="hyaE"
                     /locus_tag="ACHK49_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO9261847.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVLLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            6338..7195
                     /gene="hyaF"
                     /locus_tag="ACHK49_001170"
     CDS             6338..7195
                     /gene="hyaF"
                     /locus_tag="ACHK49_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO9261848.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            7329..8874
                     /gene="appC"
                     /locus_tag="ACHK49_001171"
                     /pseudo
     CDS             7329..8874
                     /gene="appC"
                     /locus_tag="ACHK49_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
     gene            8886..10022
                     /gene="appB"
                     /locus_tag="ACHK49_001172"
     CDS             8886..10022
                     /gene="appB"
                     /locus_tag="ACHK49_001172"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO9261849.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPSAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            10035..10127
                     /gene="cbdX"
                     /locus_tag="ACHK49_001173"
     CDS             10035..10127
                     /gene="cbdX"
                     /locus_tag="ACHK49_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO9261850.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            10207..11505
                     /gene="appA"
                     /locus_tag="ACHK49_001174"
     CDS             10207..11505
                     /gene="appA"
                     /locus_tag="ACHK49_001174"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO9261851.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(11620..13800)
                     /gene="etk"
                     /locus_tag="ACHK49_001175"
     CDS             complement(11620..13800)
                     /gene="etk"
                     /locus_tag="ACHK49_001175"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO9261852.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(13820..14266)
                     /gene="etp"
                     /locus_tag="ACHK49_001176"
                     /gene_synonym="yccY"
     CDS             complement(13820..14266)
                     /gene="etp"
                     /locus_tag="ACHK49_001176"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO9261853.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(14254..15393)
                     /gene="gfcE"
                     /locus_tag="ACHK49_001177"
     CDS             complement(14254..15393)
                     /gene="gfcE"
                     /locus_tag="ACHK49_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO9261854.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(15439..17535)
                     /gene="gfcD"
                     /locus_tag="ACHK49_001178"
     CDS             complement(15439..17535)
                     /gene="gfcD"
                     /locus_tag="ACHK49_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO9261855.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(17535..18281)
                     /gene="gfcC"
                     /locus_tag="ACHK49_001179"
     CDS             complement(17535..18281)
                     /gene="gfcC"
                     /locus_tag="ACHK49_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO9261856.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(18278..18922)
                     /gene="gfcB"
                     /locus_tag="ACHK49_001180"
     CDS             complement(18278..18922)
                     /gene="gfcB"
                     /locus_tag="ACHK49_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO9261857.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(19029..19334)
                     /gene="gfcA"
                     /locus_tag="ACHK49_001181"
     CDS             complement(19029..19334)
                     /gene="gfcA"
                     /locus_tag="ACHK49_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HFO9261858.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(19776..19988)
                     /gene="cspH"
                     /locus_tag="ACHK49_001182"
     CDS             complement(19776..19988)
                     /gene="cspH"
                     /locus_tag="ACHK49_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO9261859.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            20274..20486
                     /gene="cspG"
                     /locus_tag="ACHK49_001183"
     CDS             20274..20486
                     /gene="cspG"
                     /locus_tag="ACHK49_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO9261860.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            20497..20685
                     /gene="ymcF"
                     /locus_tag="ACHK49_001184"
     CDS             20497..20685
                     /gene="ymcF"
                     /locus_tag="ACHK49_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO9261861.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            20660..20890
                     /gene="ymcE"
                     /locus_tag="ACHK49_001185"
     CDS             20660..20890
                     /gene="ymcE"
                     /locus_tag="ACHK49_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO9261862.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            20880..21053
                     /gene="gnsA"
                     /locus_tag="ACHK49_001186"
     CDS             20880..21053
                     /gene="gnsA"
                     /locus_tag="ACHK49_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO9261863.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(21101..22174)
                     /gene="yccM"
                     /locus_tag="ACHK49_001187"
     CDS             complement(21101..22174)
                     /gene="yccM"
                     /locus_tag="ACHK49_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO9261864.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(22246..24990)
                     /gene="torS"
                     /locus_tag="ACHK49_001188"
     CDS             complement(22246..24990)
                     /gene="torS"
                     /locus_tag="ACHK49_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO9261865.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISTHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVADLEAWLNKKDLNAI
                     "
     gene            25073..26101
                     /gene="torT"
                     /locus_tag="ACHK49_001189"
     CDS             25073..26101
                     /gene="torT"
                     /locus_tag="ACHK49_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO9261866.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(26074..26766)
                     /gene="torR"
                     /locus_tag="ACHK49_001190"
     CDS             complement(26074..26766)
                     /gene="torR"
                     /locus_tag="ACHK49_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO9261867.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            26896..28068
                     /gene="torC"
                     /locus_tag="ACHK49_001191"
     CDS             26896..28068
                     /gene="torC"
                     /locus_tag="ACHK49_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO9261868.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            28068..30614
                     /gene="torA"
                     /locus_tag="ACHK49_001192"
     CDS             28068..30614
                     /gene="torA"
                     /locus_tag="ACHK49_001192"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO9261869.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            30611..31210
                     /gene="torD"
                     /locus_tag="ACHK49_001193"
     CDS             30611..31210
                     /gene="torD"
                     /locus_tag="ACHK49_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO9261870.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVGRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(31303..31608)
                     /gene="cbpM"
                     /locus_tag="ACHK49_001194"
     CDS             complement(31303..31608)
                     /gene="cbpM"
                     /locus_tag="ACHK49_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO9261871.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVTHP"
     gene            complement(31608..32528)
                     /gene="cbpA"
                     /locus_tag="ACHK49_001195"
     CDS             complement(31608..32528)
                     /gene="cbpA"
                     /locus_tag="ACHK49_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO9261872.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         7778 a         8312 c         8605 g         8074 t
ORIGIN      
        1 aggcgtttgg ccacatcagg tagaggccga tgtaaagcgt ttttttctat acttgactga
       61 gtataagatg tggaacgttg aagtcctgat tccgtataag aattttttac cttaaaaata
      121 gttgttgcac tggcaggact gttgctggga gaaagaggtg agctggtttt taccgtgtta
      181 ggtgtgcttt tgtgctgtga ggcagtgaag ctactgttgc gcgtaacact tgatatactg
      241 tgaggaatgc tggaaaaaaa agaattaatg ctatttatca tatagataac cttataagaa
      301 ctttatgtga tagtctaatt tatccgtttg catgaaatct accttttgtg aaattctcgt
      361 caatatttta ctcaaaatga tcggctgtag gaataaacta tttgatatta ttgcgttttt
      421 agagttgttt tcaatcagct atagataggg cttggcgcca gaatggagaa tatttaacga
      481 gaaatagaag ctttgtatca gacgggcttc cctgaataga gaagttaaat aatcaggatg
      541 atgctaatga atatcaggaa caaggtgagt gtcttcatgt tcactgaaac agggtgtcac
      601 aaaaaatatg gctataagat gatcaatgtg atttgaaata tttataggtt tacagggggc
      661 tgggcatgca tatcttactg caatgcagaa tatgtgctgc ttggcgcagc tctttattga
      721 gaatgttcaa tcttttgttt cagtttcgtg aaggatacgg acggtttctt gtgtaatatt
      781 atacatcgcc tgtttatctt tactgcatac tcctgcaagt gttaatgcta ctccagcata
      841 aatttctgga taactatgtg taaatatatc cagtgagttt acatgtttct ctttcattcc
      901 tgcatcacaa cgaaccttat ttatacctgt cgccattgat tggggagggc tagagccttc
      961 tgcacgaaaa tagtgatgtg acagatctga gatagctttg tatccttctg cctggttcag
     1021 gcctggagca gtccatgttt tacaaaaata ctcggcagcg taacgtgttg caagagcggc
     1081 atcagacgag gaacctgagg ttccgctcat gaccgataat ctcatccgaa taaaaatata
     1141 ccaattggta taccaagttt ataccaagag ccaaaaaaca aaggggctac catttggtaa
     1201 ccccttgatt atttggcgga agcgcagaga ttcgaactct ggaacccttt cgggtcgccg
     1261 gttttcaaga ccggtgcctt caaccgctcg gccacacttc cggaatgacg cgcactataa
     1321 acatcccgat gcggcgtgta aacccctaat ttgtttgttt gcctgaaaaa cagccaaaag
     1381 tgcattgata gcgtgaaata acagcagatt gatcatttca tcaccatgaa ttccttctct
     1441 tttactcgtt tagcaaccgg ctaaacatcc ccaccgcccg gccaaaagaa aaataggtcc
     1501 atttttatcg ctaaaagata aatccacaca gtttgtattg ttttgtgcaa aagtttcact
     1561 acgctttatt aacaatactt tctggcgacg tgtgccagtg cagaaggatg agctttcgtt
     1621 ttcagcatct cacgtgaagc gatggtttgc cttgctacag ggacgtcgct tgccgaccat
     1681 aagcgcccgg tgtcctgccg gtgtcgcaag gagaagagac gtgcgatatg aataacgagg
     1741 aaacatttta ccaggccatg cggcgtcagg gcgttacccg gcgcagcttt ctcaaatatt
     1801 gtagtctggc tgccacgtcg ctgggattag gcgcgggaat ggcaccaaag attgcctggg
     1861 cgctggagaa caaaccccgc attccggtgg tatggatcca cggtctggaa tgcacctgct
     1921 gtaccgaatc ttttatccgc tccgctcacc cactggcgaa ggacgtcatc ctttccctga
     1981 tttccctcga ttatgacgat actttgatgg ctgccgccgg aacccaggcg gaagaagtct
     2041 tcgaagacat catcacgcaa tacaatggca aatatatcct cgcagtagaa ggtaatccgc
     2101 cgctgggcga gcaggggatg ttctgtatca gcagcggtcg accgtttatt gagaaactca
     2161 aacgtgccgc tgccggagcc agcgcgatta tcgcctgggg aacctgcgcg tcctggggct
     2221 gcgtgcaggc cgcgcggccc aatccgacgc aggcaacgcc tatcgacaaa gtcatcaccg
     2281 acaaacccat tatcaaagta cctggctgcc cgccgatccc ggatgtgatg agcgccatca
     2341 ttacttacat ggtgaccttt gatcgcttgc cagatgtcga cagaatgggc cgtccgctga
     2401 tgttctatgg tcagcgaatc cacgataaat gctatcgccg cgcccacttc gacgccggag
     2461 agttcgtcca gagttgggat gatgacgctg cccgcaaagg ttactgcctg tacaaaatgg
     2521 gctgcaaagg gcctaccacc tataacgcct gttcttccac acgctggaat gatggcgttt
     2581 ctttcccaat ccagtctggt cacggctgcc tgggctgtgc ggaaaatggt ttctgggatc
     2641 gcggttcgtt ctacagccgc gtggtcgata ttccgcaaat gggtactcat tccaccgccg
     2701 ataccgtcgg tttaaccgcg cttggcgtgg tggcagcggc tgttggtgtg cacgcagtcg
     2761 ccagcgccgt tgaccagcgc agacgtcata accagcaacc tacagaaacc gaacatcagc
     2821 caggcaatga ggataaacag gcatgagcac tcagtacgaa actcagggat acaccatcaa
     2881 taatgccgga cgccgcctgg tggtcgaccc gattacgcgc atcgaaggcc acatgcgctg
     2941 cgaagtgaat attaacgatc agaatgtgat caccaatgcc gtctcctgcg gcaccatgtt
     3001 tcgcgggctg gagatcatcc tacaagggcg cgacccgcgc gatgcgtggg cgttcgttga
     3061 acgtatctgc ggcgtctgta ctggcgtaca cgccctggct tcggtttacg ccatcgaaga
     3121 tgctatcggt attaaagtgc cggacaacgc aaatatcatc cgcaacatta tgctggcaac
     3181 gctctggtgc cacgatcatc tggtgcactt ctatcagctt gccgggatgg actggatcga
     3241 tgtgttagat gcgctgaaag ccgacccgcg gaaaacctcc gaactggcgc aaagtctctc
     3301 ctcttggccg aaatcatccc ctggctattt cttcgacgta caaaaccgcc tgaaaaaatt
     3361 tgttgaaggc gggcagttgg ggatcttccg caatggctac tgggggcacc cgcagtacaa
     3421 actgccgcca gaagctaacc tgatgggctt tgcccactat ctcgaagctc tcgatttcca
     3481 gcgtgaaatt gtcaaaatcc acgcggtctt tggcggtaaa aacccgcatc caaactggat
     3541 tgtcggcggg atgccttgcg ccatcaacat tgacgaaagc ggcgcggtcg gggcagtcaa
     3601 tatggaacgc ctgaacctgg tgcagtcaat tatcacccgc acggcggact tcattaacaa
     3661 cgtgatgatc cccgacgcct tagccatcgg tcagttcaac aaaccgtgga gcgaaatcgg
     3721 cactggtctt tctgataaat gcgttctcag ctacggcgca ttcccggata ttgccaacga
     3781 ctttggcgag aaaagtctgc tgatgcctgg cggcgcggtg attaacggcg acttcaacaa
     3841 tgtgctgcca gtggatttgg ttgatccgca gcaggtgcag gagtttgtcg accacgcctg
     3901 gtatcgatat cccaacgatc aggtcgggcg tcatccgttc gatggcatca ccgacccgtg
     3961 gtacaacccc ggcgatgtca aaggcagcga taccaacatt cagcagctga atgaacagga
     4021 acgctactcg tggatcaaag cgccacgctg gcgcggtaac gcgatggaag tggggccgct
     4081 ggcgcgcacg ttaatcgctt atcacaaagg cgatgctgcg accgttgagt cggtcgatcg
     4141 catgatgtcg gcgttgaacc tgccgctttc cggtatccag tcaacgttag gccgcatttt
     4201 gtgccgcgcg cacgaagcgc agtgggccgc aggtaagttg cagtatttct tcgacaagct
     4261 gatgaccaac ctgaaaaacg gcaatctcgc cactgcttcc acggaaaaat gggaacctgc
     4321 aacctggccg acagagtgcc gtggtgtcgg ttttaccgaa gcgccgcgcg gggcgttagg
     4381 ccactgggcc gccattcgcg atggcaagat tgatctctac cagtgcgtgg tgccgaccac
     4441 ctggaacgcc agcccgcgcg atcccaaagg gcagattggc gcttatgaag cggcgctgat
     4501 gaacaccaaa atggcgatcc ccgagcaacc gctggagatc ctgcgtactc tgcacagctt
     4561 tgacccgtgc ctcgcctgtt caacacacgt gctgggcgac gacggtagcg agctgatctc
     4621 tgtgcaggtg cgttaacagc gaaggagaat catcatgcaa cagaaaagcg acaacgttgt
     4681 cagccactat gtctttgaag cgccagtccg catctggcac tggttgacgg tgttatgcat
     4741 ggcggtgttg atggtcaccg ggtactttat cggcaagccg ctaccttccg tcagcggcga
     4801 ggcgacgtat ctgttctata tgggctacat caggttaatt cacttcagcg ccgggatggt
     4861 ttttaccgtg gttttgctga tgcggatcta ctgggctttt gttggcaatc gatactcccg
     4921 cgagctgttt atcgtgccgg tatggcgtaa aagctggtgg cagggcgtgt ggtatgaaat
     4981 ccgctggtat ctgtttctgg caaaacgtcc gagtgccgat ataggccata atcccatcgc
     5041 ccaggcggcg atgttcggct atttcctgat gtcggtcttt atgatcatca ctggttttgc
     5101 gctgtatagc gaacacagcc agtacgctat ttttgcgccg ttccgttatg tggtggaatt
     5161 tttctactgg atgggtggca actcaatgga cattcacagc tggcatcggc tggggatgtg
     5221 gctgattggc gcgtttgtga tcggtcatgt ctacatggcg ctgcgtgaag acatcatgtc
     5281 cgacgacacg gtgatctcca ccatggtcaa cggctaccgt agccacaaat ttggcaaaat
     5341 aagtaacaag gagcgttcat gagcgagcaa cgcgtggtgg tcatggggct gggcaacctg
     5401 ctgtgggccg atgaaggctt cggcgtgcgg gtggcggaac ggctgtatgc ccattaccac
     5461 tggcccgagt atgtggagat tgtcgatggc ggtactcagg gactgaactt gctggggtat
     5521 gtcgaaagcg ccagccatct gttgattctc gatgccattg actacgggct ggaacctgga
     5581 acgctgcgaa cctatgccgg agaacgcatt ccggcttatc tcagcgcgaa gaaaatgagc
     5641 ctgcatcaga acagtttctc cgaagtgttg gcgctggcgg atatccgcgg acatctgcca
     5701 gcacatattg ccctcgtcgg tctgcaaccc gcaatgctcg acgactacgg cggtagcctg
     5761 agcgaactgg cacgggagca actgcccgct gcggaacagg cggcgctggc gcagctggct
     5821 gcgtggggaa ttgtgccgca accggctaat gaatcgcgct gtctcaatta tgactgtctg
     5881 tcgatggaaa attacgaagg cgttcgcttg cgccagtacc ggatgacaca ggaggagcag
     5941 ggatgagcaa cgacacgcca tttgatgcgt tgtggcaacg aatgctggcg cgcggctgga
     6001 cgccagtcag tgaatcccgt cttgacgact ggcttacgca agcgccagac ggcgtgctgt
     6061 tattaagcag tgacccgaaa cgcacgccag aggtcagcga taatccggta atgattggcg
     6121 aattactgcg cgagtttccc gactatacat ggcaggtggc gattgctgac cttgagcaga
     6181 gcgaagccat cggcgatcgc tttggcgtct ttcgctttcc tgccacttta gtgtttaccg
     6241 gcggaaacta tcgcggcgtg ctgaatggta ttcacccgtg ggcggaactg ataaacctga
     6301 tgcgcgggct tgtcgaaccg cagcaggagc gtgcctcatg agcgaaacat ttttccatct
     6361 gctggggcca ggaacgcaac cgaacgatga cagtttcagc atgaatccac tgccgatcac
     6421 ctgtcaggtg aatgatgaac cgagtatggc ggccctggag caatgtgctc acagcccgca
     6481 ggtgattgcg ctgttaaacg agttacaaca tcaactaagc gaacgccaac cgccgttggg
     6541 cgaggtgctg gcagtcgatc tgttaaatct caacgccgac gatcgtcact ttatcaatac
     6601 gcttctcggg gaaggggaag tgtcagtgcg cattcagcag gctgacgaca gtgaaagtga
     6661 aatacaggag gcgatcttct gcggattatg gcgggtgcgc agacgtcgcg gcgaaaagtt
     6721 gctggaggac aaactggagg ctggctgcgc gccgctggcg ttgtggcagg cggcaacgca
     6781 aaatctcttg ccgacagatt cgctgttacc gccgcccatt gatggcctga tgaatggcct
     6841 accgttggcg catgagttac tggcacatgt acgtaacccc gacgcgcagc cgcacagcat
     6901 taatctgacg caattaccca tcagcgaggc tgatcggctt tttctctcac gtctctgtgg
     6961 gccgggaaat attcagattc gtaccattgg ctatggcgag agctatatca acgccacggg
     7021 gttacgccat gtctggcatt tacgctgtac ggacacctta aaaggcccgt tactggaaag
     7081 ttatgaaatc tgcccaatac cggaagtggt gctggcagcg ccagaagatt tggtcgactc
     7141 tgcgcagcgg cttagcgagg tatgtcagtg gctggcggaa gctgcaccga cgtaaaaaga
     7201 cggtaagtat cgctttcagt cttatgaata tcgcaatcgg cgaatacctc tggtcgtaga
     7261 gtttcaggat aaagagggag atctaccatt atcgggttat ttttctctct tcgcctacag
     7321 gagtgcgcat gtgggatgtc attgatttat cgcgctggca gtttgctctg acctgcgctg
     7381 tatcactttt tatttgtacc ccttaccctg gggctgattt ttttgctggc tatcatggaa
     7441 accatttacg tggtcaccgg caaaacaatc taccgcgata tgacgcgctt ctggggtaag
     7501 ctcttcggta tcaattttgc tcttggcgtg gctaccggcc tgaccatgga gtttcagttt
     7561 ggtactaact ggtcattcta ttccaactat gtgggcgata tttttggcgc accactggcg
     7621 atggaagcat taatggcctt cttcctcgaa tccacctttg tcgggctgtt cttcttcggc
     7681 tggcaacggc tgaataaata ccagcacctg ctggtgacgt ggctggtggc gttcggttca
     7741 aatctctctg cgttgtggat attgaatgcc aacggttgga tgcaataccc gaccggtgcg
     7801 cattttgata tcgacaccct gcgtatggag atgaccagct tcagcgagct ggtctttaac
     7861 ccggtcagcc aggtgaaatt tgtgcacacc gtaatggcgg gctacgtgac cggggccatg
     7921 tttattatgg cgatcagcgc ctggtattta ctgcgcggac gggagcgcga tgtcgcatta
     7981 cgttcgtttg ccatcggttc cgtcttcggt actctggcga ttatcggtac cctgcaactc
     8041 ggagacagtt ctgcgtatga agtcgcgcaa gtacaaccgg taaaactggc ggcgatggaa
     8101 ggggagtggc aaacggaacc tgcacctgca ccgttccatg tggttgcctg gccggaacag
     8161 gatcaagagc gtaacgcctt tgccatcaaa attcccgcgc tgctagggat cctcgccact
     8221 cactcattag ataaacccgt gccgggtctg aagaatttga tggctgaaac ctacccacgc
     8281 ttgcaacgcg gacgtatggc ctggctgtta atgcaggaaa tatcgcaagg caatcgtgag
     8341 ccgcatgtgt tgcaggcatt ccgggaactg gaaggtgacc tgggctacgg catgttgctc
     8401 tcccgctatg cgccggatat gaatcatgtc acagccgcac agtaccaggc ggcgatgcgt
     8461 ggcgcgatac ctcaggttgc gccggtattc tggagtttcc gcatcatggt gggctgtggt
     8521 tccctgctgc tactggtgat gctgattgcg cttgtccaga cgctgcgtgg caaaatcgac
     8581 cagcatcgct gggtgctgaa aatggcgctc tggagtttgc cgttgccgtg gattgcgatt
     8641 gaagccgggt ggtttatgac cgagtttggt cgtcagccgt gggcgataca ggacatctta
     8701 ccgacatact ccgcgcactc cgctttaacc acaggacaac tggctttctc actgatcatg
     8761 atcgtagggc tttacaccct gttcttaatc gccgaagtct acctgatgca gaaatatgcc
     8821 cgtctggggc cgagcgcgat gcagagtgaa caaccgacgc agcaacaggg gtaaaggaga
     8881 aaatcatgtt tgattatgaa acattgcgct tcatctggtg gctgctgatt ggcgtgatcc
     8941 tggtggtctt tatgatctcc gacggatttg acatggggat cggctgtctg ctgccgctgg
     9001 tggcgcgtaa tgatgatgaa cgccggatag tgataaacag cgttggtgca cactgggaag
     9061 gcaaccaggt ctggttgatc ctcgctggtg gggcattatt tgccgcctgg cccagagtgt
     9121 atgcagcggc gttttccggc ttttatgtgg cgatgatcct ggtgctgtgc tcactgttct
     9181 tccgcccgct ggcctttgat tatcgcggaa aaatcgccga tgcacgctgg cgtaaaatgt
     9241 gggacgccgg tctggtcatc ggcagtctgg tgccgccggt agtcttcggt atcgccttcg
     9301 gcaacttgtt gctcggcgtg ccgtttgcct tcacaccgca attacgcgtg gagtatctcg
     9361 gcagcttctg gcaactgctg acgccattcc ctttattgtg cggattgctc agccttggga
     9421 tggtgatttt gcaaggtggc gtctggttac aactgaaaac tgttggtgtg attcatctgc
     9481 gttcacagct ggccaccaaa cgcgctgcac tgttggtgat gctgtgcttt ttgctggcgg
     9541 gttactggct gtgggtcggt attgatggct ttgtactgct cgcccaggat gctaacggtc
     9601 cttccaatcc gttaatgaaa ctggtggcag tgctacctag tgcctggatg aataatttcg
     9661 tcgaatcgcc cgttttgtgg atcttcccgc tgctggggtt cttctgccca ttgctgacgg
     9721 tgatggcgat ttatcgtggt cgcccgggtt ggggattttt aatggcgtca ttgatgcaat
     9781 ttggcgtgat tttcacggca ggcatcacgc tgttcccctt tgtcatgccg tcaagcgtga
     9841 gtccaatctc cagcctgacg ttgtgggaca gcacttccag tcagctgacg ctgagcatta
     9901 tgttggtaat cgtgctgata tttttgccca ttgtgttgct ctacactctc tggagctact
     9961 acaaaatgtg gggacgcatg acaacagaaa ctctccgccg taacgaaaac gagttgtact
    10021 aaggagcagg aacaatgtgg tatttacttt ggttcgtcgg cattttgttg atgtgttcgc
    10081 tctccaccct tgtgttggta tggctggacc cgcgtctgaa aagttaacga acgtaggcct
    10141 gatgcggcgc attagcatcg catcaggcaa tcaataatgt cagatatgaa aagcggaaac
    10201 atatcgatga aagcgatctt aatcccattt ttatctcttc tgattccgtt aaccccgcaa
    10261 tctgcattcg ctcagagtga gccggagctg aagctggaaa gtgtggtgat tgtcagtcgt
    10321 catggtgtgc gtgctccaac caaggccacg caactgatgc aggatgtcac cccagacgca
    10381 tggccaacct ggccggtaaa actgggttgg ctgacaccgc gcggtggtga gctaatcgcc
    10441 tatctcggac attaccaacg ccagcgtctg gtagccgacg gattgctggc gaaaaagggc
    10501 tgcccgcagt ctggtcaggt cgcgattatt gctgatgtcg acgagcgtac ccgtaaaaca
    10561 ggcgaagcct tcgccgccgg gctggcacct gactgtgcaa taaccgtaca tacccaggca
    10621 gatacgtcca gtcccgatcc gttatttaat cctctaaaaa ctggcgtttg ccaactggat
    10681 aatgcgaacg tgactgacgc gatcctcagc agggcaggag ggtcaattgc tgactttacc
    10741 gggcatcggc aaacggcgtt tcgcgaactg gaacgggtgc ttaattttcc gcaatcaaac
    10801 ttgtgcctta aacgtgagaa acaggacgaa agctgttcat taacgcaggc attaccatcg
    10861 gaactcaagg tgagcgccga caatgtctca ttaaccggtg cggtaagcct cgcatcaatg
    10921 ctgacggaga tatttctcct gcaacaagca cagggaatgc cggagccggg gtggggaagg
    10981 atcaccgatt cacaccagtg gaacaccttg ctaagtttgc ataacgcgca attttatttg
    11041 ctacaacgca cgccagaggt tgcccgcagc cgcgccaccc cgttattaga tttgatcaag
    11101 acagcgttga cgccccatcc accgcaaaaa caggcgtatg gtgtgacatt acccacttca
    11161 gtgctgttta tcgccggaca cgatactaat ctggcaaatc tcggcggcgc actggagctc
    11221 aactggacgc ttcccggtca gccggataac acgccgccag gtggtgaact ggtgtttgaa
    11281 cgctggcgtc ggctaagcga taacagccag tggattcagg tttcgctggt cttccagact
    11341 ttacagcaga tgcgtgataa aacgccgctg tcattaaata cgccgcccgg agaggtgaaa
    11401 ctgaccctgg caggatgtga agagcgaaat gcgcagggca tgtgttcgtt ggcaggtttt
    11461 acgcaaatcg tgaatgaagc acgcataccg gcgtgcagtt tgtaatgcat aaaaaagagc
    11521 attcagttac ctgaatgctc tgaggctgat gacaaacgaa gaactgtcta atgcgtagac
    11581 cggaaaaggc gttcacgccg catccggcca ctttcagttt tactctttct cggagtaact
    11641 ataaccgtaa tagttatagc cgtaactgta agcggtgctg gcgcgtttaa tcacaccatt
    11701 gaggatagcg cctttaatat tgacgcctgc ctgttccaga cgctgcattg acaaactcac
    11761 ctctttggcg gtgttcaagc caaaacgcgc aaccagcagg ctggtgccaa cagaacgccc
    11821 cacgaccgcg gcatcactca ccgccagcat cggcggcgta tcgacaatca ccagatcgta
    11881 atggtcgttc gcccattcca gtaattgacg catccgatcg cgcatcagca gttcagacgg
    11941 gttaggtggc acctgaccgc gagtaatcac atcaaagcct cctttgccaa aatgctggat
    12001 cactttgttg agctcatctt tacctgccag atattccgac aagccatgtt cattactcac
    12061 ggtaaacagg ttatgcgaat aaccacggcg taagtcggca tcaataaata acactttttg
    12121 atcggactgg gcgatcaccg ctgccagagt tgaactgaca aacgttttac cactgtctgg
    12181 cgtcgcaccg gtgatcatca gaatgttatt ctccgtctcc atcatagcga aatgcagact
    12241 ggttcgtagc gcacgtacgg cttccacagc agaatccgcc gggttatcca ccgccaggaa
    12301 ggggatattt ttagtacgat ggcgctgctg attagaaaat aaatttttct tacgcagacg
    12361 ggtgcgttta tccagccact cggacattgg gatagtggca taaacgctga tgccgtgctc
    12421 ttccagttgt tccggggctt ctacaccacg acgcaacatc gcacgcgcca gcacggcacc
    12481 cacagaaata aacaggccaa gaataaaacc aagcaccaca ttcaacgctt ttttcggttt
    12541 cactggctgc ggctgagtga ctgccgggtc gataatccgc acgttaccaa tggcactgga
    12601 tttcgaaata ctcaactcct gctggcggtt aagtaattgc agatataccg cacggcccgc
    12661 ttctacgtca cgacttaaac gcaacacttc ctgttgggtg gaaggcattg ccgatacccg
    12721 cttattcagg cgtttgcgtt cttgctccag cgtctggcgt ttttccagca gcgcacgata
    12781 agttgggtga tctttcttat acagctggga gatctctgcc tcgcggaagg tcagctcatt
    12841 gagttgatta tcaacgttca caatctgctc aagaacggct ttggcttcca ggttaaggtc
    12901 aaccgaatcg cgctgctggc gataaacgtt gagtttttct tccgcctggt ccagctcgct
    12961 gcgcacttca ggtaactggc gctgtaagaa ttcaaggctt tgtgaatcct gcgccgcctg
    13021 gcgagcgata ttctgttgca aatagttgtt agcgatgctg ttcagaatac gagtaatcaa
    13081 ctggggatca tcaccagtca tggtaagttc cagcatcccg ctttctttac tgcgttcgct
    13141 aacggtaaag gtttcctgca atgcgttaat cgcttccagt tcggtacgct ggctcaggac
    13201 aaactgtgtt cctggtttgg ccttaatgtc cgcgatagtc agcgcaacgc catctttttc
    13261 cagacgctgg ccgaccatac cattgacggt gaactcttca ccttccagtg tatagtggcc
    13321 gttttcccca accgtgagtg tcagttgctg atcctgacca ttcagttgtg gaatatgcat
    13381 ccagctgatc gccagctcac ctggtttttc tttggttaat ctcgcccagc cgcgacccac
    13441 aatcggaaaa tacttctgct caactatgtc gcgcagattc agttcagcaa tggttttacc
    13501 gagaatcatg cgcgattgca gcagttggat ctccggtgca gactcgggcg atgagttagg
    13561 gatcatatcg ctcaggccgc tgagaatggc gttgccctgt ttttgctcaa cctggaccag
    13621 agtatctgcc tgataaattg gtgtgcttaa cagcgagtaa gcgacagcga tcagcgtgaa
    13681 taacgcggtc acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag
    13741 cagatcgatc tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat
    13801 cgtattttat aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct
    13861 ccaacatacc gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct
    13921 ctttctgttc cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg
    13981 cggtaacctg agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca
    14041 tctcagcagt gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa
    14101 catctgccgc tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga
    14161 cttttacgcc cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt
    14221 tgccggtaca aaccaccagg attgagttaa attttagttg ggccatgttt gaatccaccg
    14281 tgaagtctct gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg
    14341 gttccaacga gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa
    14401 ttccgttccg agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat
    14461 tttgccattc tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac
    14521 atcctgattc ataccctcag cgttacccag cgcttccgcc agggtcatac cgctgcggtc
    14581 cattttcagc gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt
    14641 gcgcggaata aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg
    14701 ctgcatcagc gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt
    14761 gcgccagtct gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag
    14821 aggaatattg gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt
    14881 ttgcgagcgg aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatccag
    14941 acgcgctgta atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc
    15001 aatatagggg tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct
    15061 acggtattgc cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac
    15121 atccccaatg ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc
    15181 ctgcgacata atcggtttgg cgcgcaactg atcaatcaat cctggcgtga cgggatagat
    15241 attgaccatt ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact
    15301 ggtcgataaa ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt
    15361 cagtaccgaa aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat
    15421 gttatgaatt acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggata
    15481 gagctggtat ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac
    15541 caccgcgcgg ttgcggttag gtccaatggt catcaagtcg aatggaatcg agatataaaa
    15601 acctttacta aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca
    15661 tacccctacc gccacgccgc tgtcaaagcg tttggcgacg tcgatcgttg cgcctttatc
    15721 ttttgccaga tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt
    15781 ccagtaagcc gtcacgaaac ctgttggcgt ggaataatcg gtgaaacgca tcatgttgtc
    15841 ccagtcacgt tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg
    15901 gcgataaagc agctcggaac cgacaccggc gtacatcgtt tccagataac cgccatacac
    15961 ctggccatag aaaccattgc ctaagtcggc aaagtagttc gcctgcaagt tgttgagata
    16021 aacgtcattg cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc
    16081 gggcaacagc gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgcc
    16141 atcaagcagc aggtggtcgg taaaccagta gcgggcactg gacatcagcc ccagctggaa
    16201 catatagaaa tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct
    16261 aaagcgatct tcacgaatac ggtagccccg accaaaggca gaaagatctt ctgcttcaac
    16321 acgttgttgt tgaagttgct ctgattgacc aggcgctgta ccagccagct gtttgcgcag
    16381 gctggctacg tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct
    16441 aatcttctca acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc
    16501 ttcgcgagaa tcacggtatt tgtattgttg accagacata tacagcgtct tatcgcgcag
    16561 ctgaatttcc ggtgcttcaa aacctgcgtt gtacttcagc gcggtaagtt ggttcgctac
    16621 tgtggtgtac tgcaatcctt cagattcagg cgcaggttga tatgccggtt ttggcgtatc
    16681 gcgcagggca gggcgcagat cgttgaaatt ggtccgtaac gtgaagccaa acatcaacgt
    16741 gttaccgcgt tcataactca ggttgagatc tgcccagctg gcagcgcgat aaactgcgcc
    16801 gacgttgaaa tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc
    16861 atcgtattcg agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa
    16921 gatggaagcc gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc
    16981 tgcgcgatga caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt
    17041 accggcgtag ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc
    17101 caccagatac tcaccgtcaa acagacccgt accagcaata tcacgtttac caaacgccac
    17161 ttgcggtagc cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt
    17221 gtatgactga tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt
    17281 ataacgaatg gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta
    17341 ttgatcgtta tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg
    17401 catctgcaac aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta
    17461 ggttaatact tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata
    17521 agaattcttc ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt
    17581 aaatcagcat atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca
    17641 ggcggtggtt ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc
    17701 tcaggcgtaa tcaccataac gttattcttg tccgcgcctg caaggcgggg gtgatcttgc
    17761 agataatcgg taacgctacg gcctgcctgc cagggcaatt gcccggcccc agaaaccgca
    17821 cccagcagag taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac
    17881 gggggattgc tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga
    17941 ccagtgatat tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg
    18001 tcgtcagctt ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc
    18061 gctttagctt tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc
    18121 agttgcggtt gtgtcaccaa ctggacaaca ttttccatgg ggccaacgga aagtgtctgt
    18181 tgttcgccag gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg
    18241 taaagtacgc tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg
    18301 tcatttctac gggaataacc cctgcgccta acatttgccg actttgacga acctggccag
    18361 tagccgaatc gacccagaat gtgttttgcc actgtttttc tggccgcgtg gaagtcacct
    18421 cttcctgcca gacattacac agcacattgc gacccgcaat attcagcgtc tctaatccag
    18481 caaaggaaaa agtagaactc agggtcgcag agcgaaaatg gttgtcttca ctccagagaa
    18541 tatcgcgcgt ccagcggctg ccatctttta ttgccagcgc gttacgcaga gggtcctgcc
    18601 cggaatttgt cacttccagc agattattat taagtttgac ggtttttaaa agacgtccat
    18661 tgtgggtaac cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat
    18721 atcccagtac cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag
    18781 gcaacgcctg gatctgctga tccgctacgg tgatatcctg attatcgaaa aggctggcgc
    18841 gaaatgtatc gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa
    18901 tcgataaaat aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc
    18961 tctaaagccc ggtacgaata tcgcaaccgg gtatcattta actttaattt cgacgcgtta
    19021 aagcagtatt agcgtgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca
    19081 ctgtcatttg ctgcaacaac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca
    19141 actgtcgccg tgccgacagc gccagcagcc acgccaccag tggtagtagc agtcgtggtt
    19201 gcgccggtag tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgtt
    19261 gcttcagggg cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa
    19321 agcttgtgtt tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc
    19381 aggattgtct actttgagta taacatcctt gtgaaatctc cctttcctca taccgcttgc
    19441 actatatact gcgggtagga aaggcgcgca gagggaaata taagattgtt aactatcccc
    19501 ctctggaaat acgacttgtg agcataagat aaatcttagc aaaatacaaa ttaaagataa
    19561 ggaaattgtt acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactatag
    19621 ctggctgtta aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag
    19681 tttctttgtg gaaaagtaac tggtgttata atgtatgatg ttgactattg cactattcca
    19741 ataaaaccag tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct
    19801 gttggtcctc gcaggccatt tacgcggcaa aattccacac gtaatcctgg tataagcact
    19861 tctgcgtcgc ggggagtgaa tgcggaaata tggacctgaa cttctttacg accgtcggag
    19921 gggataatga atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta
    19981 cgggacaaac aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata
    20041 aagccagcga tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat
    20101 catgcgttaa tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag
    20161 cagttctcat tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtag
    20221 tttctgtatt aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta
    20281 ataaaatgac tggtttagta aaatggttta acgcagataa aggttttggc tttatcactc
    20341 ctgatgatgg cagcaaagac gttttcgtcc atttcactgc catccagagc aatgaattcc
    20401 gcacgctgaa cgaaaatcag aaagttgaat tttctattga gcaggggcaa cgtggccccg
    20461 cggcagcgaa cgttgttacg ctctaaggtt gccgttatta ctcaacatct ccatttccgc
    20521 tgtccatgtt gtcatggttc acagtaccgt acatcggcat tcgatgtgac ggagcgaaac
    20581 cctttgggcg ctaagtgtat tttttgtaaa tcgacgatga tcacctttga taacgtcgcg
    20641 ctgcaaatac gcacggacca tgcgccgctg gatttcacaa aataatatca ggctccctcg
    20701 tggagccttt tttatatctg ccttattttt cttcaacgct gtatgtatag taagcgataa
    20761 cctgttgatt attgaatctt tcggggagat ggcttataac atttcttacc tgaccagggt
    20821 accgggaacc aacaccttac tggcgtgttg ctgtctttca agaccagaag aggttaacag
    20881 tgaatattga agagttaaaa aaacaagccg aaacggaaat cgccgacttt atcgcgcaaa
    20941 aaatcgccga gctgaacaag aatacaggga aagaagtctc tgaaattcgc ttcaccgcac
    21001 gagaaaaaat gaccgggctt gaaagttatg atgtcaaaat caaaataatg tgattttgtg
    21061 aacatcaccc gtgcgaggtg atgttccgct tgttgctaat ttagtgacca atcattggcg
    21121 cttgtggaat taagcgtcgg tacaattcat ccggtaccgg gctttgccat actcccgcat
    21181 acattgcgta accaatcacc gcaaacataa tccccagaac cagtagcgtc attaaccagc
    21241 cagacaacgc aaaggctttt ttatttgccg caggtttttg cagtgaaaag gtcaatgttg
    21301 aggctaccgg acatgactct acgcaagtca tacagccggt acattccact gttcgtacct
    21361 gaattaattt atcgaccggg atccgtgatg ggcaattttt tgcgcatttg ccacagtcga
    21421 tacaactttc ggcattgcga cgaatcttaa acggcgacaa tagcgaaacc acgcccatca
    21481 gcgcgccata tgggcaaaga taacgacacc aggcatggcg aataaacagg ctggcaatca
    21541 gcaaaacggt cacgctgatt aatgtcgcgg tccccatatg acgaaagaaa tcgagcattt
    21601 taacgtccat caccacgctg tagggcgaca acataaaata gtgaatcgcc tgagcgggca
    21661 tcaataatgc gatatagaga aaaaaactca acagcaaata cttcacgccg cgcagaggaa
    21721 tatccagcca gcggggaagg acacattgcc gaccaaacag tttgttaccg agatcgccga
    21781 ttaattcaga aagcgtacca accgggcata accatgagca aaaggccttt ttgagtaata
    21841 gactgatgac gataaaagcg accaataaca gcatcgcggc ggcgtggacg gacggtaact
    21901 gacctgttgt aaggctatat ttcagattca tcagaccggc aatcggtagc cagccttcga
    21961 tacctcccgg tctggcgaca aatgtcgtgc tacttgccgt ttcgtaatag cgcacccaat
    22021 accagaacgt gatggcaata taaatattca ttgccaacag taataattgc gtcgctttac
    22081 gccaggtcgt ggcattgcgc cagtcattcc acggtaattt gccgcccgtc gtgcctggcc
    22141 gccgctgcca gcgggttctt tttttctctg ccatgatttt gccagtccgt taagttgcat
    22201 accaaatgcc actattctag ttgttcttaa ctggctgata ttgattcaaa tcgcgttcag
    22261 gtctttctta ttcaaccatg cttccagatc ggcaacactg cgtgtaattt cttcgtgtgg
    22321 aaggggggca gataatggct gctgctccag ttgtgcgcat agctggctgg cgatacgcat
    22381 tcccaaactc gagcaactgc tttttagctg atgcgcggca cgctttattt tctcgctatc
    22441 ctgactggcg cgggcaatgt cgatttcatc gagaagcggc agggcatgtt gtgtaaataa
    22501 taccagccat tcgtggatct tctccgtccc cattaactga gcatcttcat tgagttgcga
    22561 tacatccagc gattgatcgt tattgacttg cagttggaga tagtgcgcca gtaactgacc
    22621 gagcacttca cgcggcaccg gtttagggat aatcccgcgg aataatgaac tggtacgctg
    22681 gcgcagcgtt tcgtcaatga catgggcgct aaagccaatc aaaaccagcg acggatattg
    22741 ctgtgccagt tgtcgggcaa gcgtaatgcc gtcgacatcc ggcagatcaa aatccaccag
    22801 tgcggcagca aacggttcgc tattttgcag tgtctctaaa gcctgcgcgg cattgccaac
    22861 agcaacaacc tgcgcaccac tggtgttcag catctcaacg gtaattcgct gggttagcgg
    22921 gttatcttca attaacagca aacgtaaacc gtcaagacgc accgcctgat tgactgtttt
    22981 tggcacgggt gccgtggcaa cacgtaacgg caagcgtaaa caaaaacagc ttccaacctc
    23041 cggcgtgctg gtggcgctca gttcgccgcc catcgcctgg gccagacggc tactgatagt
    23101 cagccccagc ccggtgccgc cgcgtttgcc gcttacctgc acaaatggct ggaagatttc
    23161 tgccagtttc gcgggatcaa taccgcagcc gctgtcttcc acttcgacca gccattgctc
    23221 gccatcagtg cgactacgca ggataatgta cccttcgtca gtaaaacgca gggcgttgct
    23281 caacaggttg gttataacct gacgaatacg tcgtggatcg cccattaacg cgcacggcat
    23341 atcatcggca attgccgttg ccaggcgaat cgggcgacct ttcacccgtc cgctcattaa
    23401 ttgcagggta ctttccagca gcgggcgcgg ttcaaagggc tcatcgctga ccgaaacatt
    23461 cttgccacct gcttcgatag cggaataatc gagaatatcg ttgaggatgg tcagcaacga
    23521 ttcaccagaa tcagtaattg cccgcaaatc atcacgctgg gcgttaagtg cggggttatc
    23581 tgccagcagt tgagcagtgc cgagaatacc gtacagcggt gtgcggatct catggctcat
    23641 cgccgccaga aacgccgatt ttgcctggct ggctttttct gcttccgccc gtgcctgtcg
    23701 gtgttctatc accagttcct gcaattcagc tgtacgcgct ttgacctgcg ccgccagctg
    23761 ttcgcggtgg cgattcagtg catgaacatt gctgcgaaac gcatccatca gccgcccgat
    23821 ggtatccagc tcccgtacgc cagcggtttc cgggaaaggg gagtcaatat caccgtccag
    23881 cagccgttgc agcgcctgcg tttgttcggc aagtggacgc gtgactgagc gataaaccac
    23941 gcgccagagg atcagaatca gtgcgcaaag agaaactatt cccagcaata acaggctgta
    24001 ttgcccgcgt gcactcgctt tttccagatg cgccagtccg tgctgattac gcagctcaat
    24061 ggtatcgacc agctgactga cttcgctact aaactgcgcg aactgggcga tgttattttg
    24121 ggcgagagtt tgcaggtgag tgctgatttc actgtcctgc tgatacagcg ccagtagatc
    24181 gctatattgg ctaacggtag ttaacgttgt tgcgacctgc gcacgaactc ccggatcttc
    24241 aatgcgtatt tgccgacgtt gcagaatttt caccgcatta ttgagctgct tttccagcgt
    24301 tggtgcattt ttctggatct gctccagccc cagattcatc accatttgct gcacccgcag
    24361 agcgctaagg cgcagttcat tcatctggtt aacatactca agatcgatat caatcagccg
    24421 atcgagtgca ctttcagcag cctgacgctg atcttgttcg atcaaatcgt aaatcccggc
    24481 ctgggtcgct ccagcggaag ttgtcgcatt attcgcctga ccttgcgcca ggcgtgcgat
    24541 ctcatcggcg gcagcgacta tctgctgact gagttgccgt tgttgctggc gtagttgcag
    24601 acgccgcccc accagttccc cttgctgacg taatgaacgg gagatctcct gctcctgttg
    24661 ttcaatagcg gtggtatcaa aaccttgttc ccgtaacgct tgcagcaacg cattaatctt
    24721 caggctttgt gcggtgagca ttcgcccctg cgcctgccac atcttttcgt tatcggcact
    24781 ggtcaggttc tgcgcggcga aaagttccca ggcgctggct tcgctcaact ggcgcgccat
    24841 attcatggta ggaatcaatg cctgagtgtt gtctttttcc acctggctga taaagcgcag
    24901 gttgtaccat cccaccaggg tactggtcag ggttaacagc gccatcaggg caaagcccat
    24961 ccagagtctt cgggtcaggg ttaaattcac ggtcggtgca cttaaggtga aaaaggttga
    25021 gtcgcaaagc ggaatgcatc tagcataaag ccttattatt gatgaggcta tcatgcgcgt
    25081 actgctattt ttacttcttt cccttttcat gttatcggca ttttcggctg ataacctgtt
    25141 gcgctggcat gatgcgcagc attacacggt gcaagcctct acgccgctta aagccaaacg
    25201 cgcatggaaa ctgtgcgcgc tttatcccag cctgaaagat tcatactggt tatcgttgaa
    25261 ctatggtatg caggaggcag ctcgccgcta tggtgtggat ttaaaagtgc tggaggcagg
    25321 cggctacagc cagttggcta cccagcaagc acaaatcgac cagtgtaaac agtggggcgc
    25381 agaggccatt ttgctcggta gtagcactac gtcatttccc gacctgcaaa agcaggtagc
    25441 aaatctgccg gtgatcgaac tggtaaatgc tcttgatgat cccctggtga aaagccgcgt
    25501 tggtgtaccc tggtttcaga tgggctatca acctgggcga tatttggtgc aatgggcgca
    25561 cggtaaacca ctgaacgtgc tgttgatgcc cggacccgat aatgccgggg gcagtaaaga
    25621 gatggtcgag ggttttcgcg cagccattgc cggaagcccg gtgcgtattg ttgatattgc
    25681 gcttggtgat aacgatattg aaatccagcg taacctgttg caggagatgc tggaacgcca
    25741 cccagaaatc gacgtcgttg ccggaacggc cattgcggca gaggcggcaa tgggtgaagg
    25801 gcgtaacctg aaaacgccgc ttaccgtggt gtcgttttat ctttcacatc aggtgtatcg
    25861 cgggctgaag cggggaagag tgattatggc tgccagcgat caaatggtct ggcaggggga
    25921 actggcggtt gagcaggcca tcaggcaatt acaggggcaa tcggtgtctg ataatgtcag
    25981 cccaccgatt ttagttctga cgccgaaaaa tgccgaccgt gaacatattc gccgctcgct
    26041 gtcaccaggg ggatttcgtc cggtctattt ttatcagcac acatcagcgg ctaagaaata
    26101 accttcacca tgttgcgtca ccagtaaatc cgcgctgagt ttatgacgta aacgacgaat
    26161 taacacatcg acggtgcgca ggtcagggtt ttccacccga cgcgcagaaa gcatacgtag
    26221 cagacgttca cggctgagaa tttcgcccgg attcgtcaca aatgccacca acatttcata
    26281 ctctgcgcgg gtcagtttaa tcggctcgcc atcccgctcc agcgtatggc gcgacacatt
    26341 caggcaataa ccggcaaagc gatagcagtt gtcctgagtg tgcggttgag cttgtcgcgc
    26401 gaggtcgatt cgccagagca gatttttcac ccgtactacc agttcgcgca gttccagcgg
    26461 tttggtgacg taatcgtctg cgcccatttc cagcccaaca atacggtcaa tccgatcgct
    26521 gcgtccggta accagaataa tccccaccgt tgagcgttct cgcagggcgc gggttaacat
    26581 caggccattt tcatcgggta agttgatatc cagcagaatt aaatctaccg gctgattctg
    26641 cataatttcc cgcagcccgg caccgctcgc tgtaacggaa acggtatacc cctcctgagt
    26701 gaagtaagat tgtaatcgcg cctgggtaac cggctcatct tcaacaataa caatgtgatg
    26761 tggcatcaga gggttttact cattctgttc atatctgttc atattctgcc gtaagccgtt
    26821 catcctgacc agtgccgctg ttcatatttg ctcattaaga tcgcttcact aaaccataat
    26881 tttacagggg ctattatgcg gaaactctgg aacgcgctac gccgacccag tgctcgttgg
    26941 tcggtactgg cgctggtcgc aattgggatt gtgattggca ttgcgctgat tgtattgcca
    27001 cacgttggga tcaaagtcac cagcacaacc gaattttgtg tcagttgcca cagtatgcaa
    27061 ccggtgtatg aagaatataa acagtcggtg catttccaga acgcctccgg cgtgcgagct
    27121 gaatgccatg actgtcatat cccgccggat attccaggca tggtgaagcg caaactggaa
    27181 gcgagcaatg atatctacca gacctttatt gctcactcca ttgatacacc tgaaaaattc
    27241 gaagccaaac gcgcggaact tgccgagcgt gaatgggcgc gaatgaaaga aaacaactcg
    27301 gcaacctgcc gctcctgcca taactacgat gcgatggatc atgcgaagca gcatcctgaa
    27361 gcggcacgtc agatgaaggt ggcagcgaaa gataatcaat cctgcatcga ctgtcataaa
    27421 ggtattgccc accagttacc ggatatgagt agcggcttcc gtaagcagtt cgatgagctg
    27481 cgcgccagtg ctaatgacag tggtgacacg ctgtactcta ttgatattaa gccaatttat
    27541 gcggcgaaag gcgataaaga agcctctggt tctctgctgc ctgcttcgga agtgaaagtc
    27601 cttaaacgtg acggcgactg gctacaaatt gaaattaccg gctggacgga aagcgccgga
    27661 cgtcagcgtg tactcaccca attcccaggt aaacgcatct ttgttgcctc gattcgtggt
    27721 gatgtgcagc agcaggtaaa aacgctggag aaaaccaccg ttgccgacac caataccgag
    27781 tggagcaagt tgcaggccac tgcgtggatg aagaaaggcg acatggtgaa cgatatcaaa
    27841 ccgatctggg cttatgcgga ttcgttgtac aacggcacct gtaaccagtg ccacggtgca
    27901 ccggaaatcg cccactttga cgctaacggt tggatcggca cgctcaacgg catgattggc
    27961 tttaccagtc tcgataaacg tgaagaacgc accttgttga aatatctgca aatgaatgcg
    28021 tctgacaccg caggtaaggc tcacggcgat aagaaggaag aaaaataatg aacaataacg
    28081 atctctttca ggcatcacgt cggcgttttc tggcacaact cggcggctta accgtcgccg
    28141 ggatgctggg gccgtcattg ttaacgccgc gccgtgcgac tgcggcgcaa gcggcgactg
    28201 acgctgtcat ctcgaaagag ggcattctta ccgggtcgca ctggggggct atccgcgcga
    28261 cggtgaagga tggtcgcttt gtggcggcaa aaccgttcga actggataaa tatccgtcga
    28321 aaatgattgc cggattgccg gatcacgtac acaacgcggc gcgtattcgt tatccgatgg
    28381 tacgcgtgga ctggctgcgt aagcgccatc tcagcgatac ctcccagcgc ggtgataacc
    28441 gttttgtgcg cgtgagctgg gatgaagccc tcgacatgtt ctatgaagaa ctggaacgcg
    28501 tgcagaaaac tcacgggccg agtgccttgc tgaccgccag tggttggcaa tcgacgggga
    28561 tgttccataa cgcttcgggg atgctggcga aagctattgc cttgcatggt aatagcgttg
    28621 gtacgggcgg agattactct accggtgctg cgcaggtgat cctgccgcgc gtggttggct
    28681 caatggaagt gtatgaacag caaacctcct ggccgctggt attgcagaac agcaaaacca
    28741 ttgtgctgtg gggctctgat ttgctgaaaa accagcaagc gaactggtgg tgcccggatc
    28801 acgatgttta tgaatattac gcgcagctaa aagcgaaagt cgccgccggt gaaattgagg
    28861 tcatcagcat cgatccggtt gtcacatcca cccatgagta tctggggcgc gagcatgtga
    28921 agcacattgc ggttaacccg caaactgatg tgccgctgca actggcgctg gcgtatacgc
    28981 tgtacagtga aaacctgtac gacaaaaact tcctcgctaa ctactgtgtg ggttttgagc
    29041 agttcctgcc gtacctgctg ggtgagaaag acggtcagcc gaaagatgcc gcatgggctg
    29101 aaaaactgac cggcattgat gccgaaacca ttcgtgggct ggcgcggcag atggcggcga
    29161 acagaacgca aattattgct ggctggtgcg tgcagcgtat gcagcacggt gaacagtggg
    29221 cgtggatgat tgtggttctg gcggcgatgc tggggcaaat tggcctgcca ggtggtggct
    29281 ttggttttgg ctggcactat aacggcgcag gcacgccggg gcgtaaaggc gttattctga
    29341 gtggtttctc cggctctacg tcgattccgc ctgttcacga caacagtgat tacaaaggct
    29401 acagcagcac cattccgatt gcccgtttta tcgatgcgat cctcgaaccg gggaaagtga
    29461 tcaactggaa cggtaaatcg gtaaaactgc cgccgctgaa aatgtgtatt tttgccggaa
    29521 ctaacccatt ccatcgccat cagcagatca accgcattat tgaaggctgg cgcaagctgg
    29581 aaacggttat cgccatagat aaccagtgga cctcaacctg ccgctttgcc gatatcgtgc
    29641 tgcctgcgac cacgcagttt gagcgtaacg atctcgacca gtacggcaac cactccaacc
    29701 gtggcattat cgccatgaaa caggtggtgc cgccgcagtt cgaggcgcgc aacgactttg
    29761 atattttccg cgagctgtgc cgtcgcttta atcgcgaaga agcctttacc gaagggctgg
    29821 acgaaatggg ctggctgaaa cgcatctggc aggaaggtgt acaacaaggc aaaggacgcg
    29881 gcgttcatct gccagcgttt gatgacttct ggaataacaa agagtacgtc gagtttgacc
    29941 atccgcagat gtttgttcgc caccaggcat tccgcgaaga tccggatctc gaaccgctgg
    30001 gcacgccgag tggcctgatt gagatctact cgaaaaccat cgccgatatg aactacgacg
    30061 attgtcaggg gcatccgatg tggtttgaga aaatcgaacg ctcccacggt gggcccggct
    30121 cgcagaagta tccgttgcat ctgcaatctg tgcatccgga tttccgactt cactcgcagt
    30181 tatgtgagtc ggaaactctg cgtcagcaat atacggtagc gggtaaagag ccagtgttca
    30241 ttaacccgca ggatgccagc gcgcgcggta ttcgtaacgg tgatgtggta cgcgtcttta
    30301 acgctcgcgg tcaggtgttg gcaggggcag tagtttctga ccgctatgca cccggcgtgg
    30361 cacgaattca cgaaggggca tggtacgatc cagataaagg cggcgagcct ggtgcgctgt
    30421 gcaaatacgg taaccccaac gtgctgacca tcgacatcgg tacttcgcag ctggcgcagg
    30481 cgaccagtgc gcacactacg ctggtggaaa ttgagaagta caatggagca gtggagcagg
    30541 tgacggcgtt taacggccct gtggagatgg tggcgcagtg cgaatatgtt cccgcgtcgc
    30601 aggtgaaatc atgaccacgc tgacagcaca acagattgcc tgtgtttacg cctggctggc
    30661 gcagttgttc tcccgtgaac tggacgatga acaactgacg caaatcgcca gtgcgcagat
    30721 ggctgaatgg ttttcgttgc tgaaaagcga accgccgctg gctgcggcgg tgaatgagct
    30781 ggaaaactgt attgccacgc tgacagtacg tgacgatgcc cgtctggaac tggccgctga
    30841 cttttgcggc ctgtttctga tgaccgacaa acaagcggcg ctgccgtatg catcggccta
    30901 caaacaggac gagcaagaga ttaaacgctt gttagttgag gcagggatgg aaaccagcgg
    30961 caatttcaac gaaccggcag atcatctggc gatctatctc gaattgctca gccatctgca
    31021 tttttcgctg ggagagggga ccgttcctgc gcgaagaatc gacagtttgc ggcaaaaaac
    31081 actgacggcg ctgtggcaat ggttaccaga gtttgttggg cgttgtcgtc agtatgacag
    31141 ctttggtttt tacgcggcac taagccagtt attgctggtg ttagtggaga gcgaccacca
    31201 aaacagataa cgtcgtttgt gcgcctgaaa agacgcgttt agcgtcgcat caggcattat
    31261 ggggcagttg ccggatgcgt catcacatcc ggcaaacaca catcacggat gagttacaaa
    31321 ccgggaaagt cgctggcgca gcaggcggtt ttcctgcttc aggtgcgcaa tatcatccat
    31381 taacgtcagc gccaccgcga tcccaggcca gtccagagcc agttcatgac gcaggcgtac
    31441 tgcgcgttgc accacgatgg cggcatggtc gtcaaatacc caggttgttt cctggatctc
    31501 acgcggttca accaccccca aaccgacaat ttcattcaac tcctcttcag agatgccggt
    31561 atgcaggcaa aattcggtaa tagtaaaagt caccgtaaca ttagccatta tgctttcccc
    31621 caatctttac gtggatcaaa agacgactgg gcgtctgcca gttgctgcca cagcgcggca
    31681 gtgttttcat ccggtttcgg cggcatcacg attttcagta ccgcatacag atcgccggtc
    31741 tgttttttgc tcaccagacc tttgccttta acgcgcaatc gttgcccggc ctggctgcct
    31801 ggcgggatag tcagcaaaat gctttctttc agtgttggaa cggtgacttt agcacccagc
    31861 gccgcttccc acgggctaac cggcactaca atttccagat catggccgac aatatcgaac
    31921 agcggatgtg gcgcaatatg aatcaccagc cacaaatcgc catttggacc gccgttttcg
    31981 cccggcgtcc cctggccttt cagacggatg cgttgaccat tgccgacgcc cgccgggatc
    32041 ttgacattca gcgttttcgg aatttcctgt tcgatcatgc caaaggcgtt ataaaccggc
    32101 aggttatagc tgatggtacg cttatgctca gtaagcgttt cttcgaggaa taccgccact
    32161 tcgatttcaa tatcgtggcc gcgtgtggcg gggcgttgac ggctctggcg ggcatgctga
    32221 ccgaaaattg acgagaagat atcgtcaaaa tcttcggcgt taaaactctg accgtcgcta
    32281 tgatggaact gacggttaaa ttgcggatcg ttgcgatgtt gccacatctg atcatactca
    32341 gcgcgacgtt gttcatcact taacacttcc caggcttcag cgacctcttt gaagcgggct
    32401 tcggcatccg gttctttgct gacatcagga tggtatttgc gggccagtcg acgataggcg
    32461 gtcttgattg tcttgagatc gtccgtcggt ttcacgccca tgatggcgta ataatccttt
    32521 aattccatag cgttatctcg cgtaaatcaa cacaaattga aggaacccct gtaaggtaac
    32581 tcctataagt gtagggtaat cctcaaaatt tcatatgcca acacagaata tgttattgaa
    32641 atcatcgcgg agaggaggtc gcctcaaggt ggggtgctga acatatttta aacaggtgaa
    32701 aaagggtgag cgatttttga tagtggaacc aggcacttta agtttaactg gggcgtcatt
    32761 atttattaa
//
LOCUS       DAZGLJ010000027        32140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000027 DAZGLJ010000000
VERSION     DAZGLJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.027"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(64..2133)
                     /gene="glyS"
                     /locus_tag="ACHK49_001196"
     CDS             complement(64..2133)
                     /gene="glyS"
                     /locus_tag="ACHK49_001196"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO9261873.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2143..3054)
                     /gene="glyQ"
                     /locus_tag="ACHK49_001197"
     CDS             complement(2143..3054)
                     /gene="glyQ"
                     /locus_tag="ACHK49_001197"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO9261874.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3149..3448)
                     /gene="ysaB"
                     /locus_tag="ACHK49_001198"
     CDS             complement(3149..3448)
                     /gene="ysaB"
                     /locus_tag="ACHK49_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO9261875.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLSMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3623..4618
                     /gene="wecH"
                     /locus_tag="ACHK49_001199"
     CDS             3623..4618
                     /gene="wecH"
                     /locus_tag="ACHK49_001199"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO9261876.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4660..5097)
                     /gene="yiaA"
                     /locus_tag="ACHK49_001200"
     CDS             complement(4660..5097)
                     /gene="yiaA"
                     /locus_tag="ACHK49_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO9261877.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(5143..5484)
                     /gene="yiaB"
                     /locus_tag="ACHK49_001201"
     CDS             complement(5143..5484)
                     /gene="yiaB"
                     /locus_tag="ACHK49_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO9261878.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5653..7107)
                     /gene="xylB"
                     /locus_tag="ACHK49_001202"
     CDS             complement(5653..7107)
                     /gene="xylB"
                     /locus_tag="ACHK49_001202"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO9261879.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7179..8501)
                     /gene="xylA"
                     /locus_tag="ACHK49_001203"
     CDS             complement(7179..8501)
                     /gene="xylA"
                     /locus_tag="ACHK49_001203"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO9261880.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMAVALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8867..9859
                     /gene="xylF"
                     /locus_tag="ACHK49_001204"
     CDS             8867..9859
                     /gene="xylF"
                     /locus_tag="ACHK49_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9261881.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            9937..11478
                     /gene="xylG"
                     /locus_tag="ACHK49_001205"
     CDS             9937..11478
                     /gene="xylG"
                     /locus_tag="ACHK49_001205"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO9261882.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAEEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11456..12637
                     /gene="xylH"
                     /locus_tag="ACHK49_001206"
     CDS             11456..12637
                     /gene="xylH"
                     /locus_tag="ACHK49_001206"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO9261883.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12715..13893
                     /gene="xylR"
                     /locus_tag="ACHK49_001207"
     CDS             12715..13893
                     /gene="xylR"
                     /locus_tag="ACHK49_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO9261884.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(14001..14825)
                     /gene="bax"
                     /locus_tag="ACHK49_001208"
     CDS             complement(14001..14825)
                     /gene="bax"
                     /locus_tag="ACHK49_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO9261885.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSNSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15145..17175
                     /gene="malS"
                     /locus_tag="ACHK49_001209"
     CDS             15145..17175
                     /gene="malS"
                     /locus_tag="ACHK49_001209"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO9261886.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTETFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTQPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASQVRVCPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDMTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17353..18606
                     /gene="avtA"
                     /locus_tag="ACHK49_001210"
     CDS             17353..18606
                     /gene="avtA"
                     /locus_tag="ACHK49_001210"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO9261887.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18758..19231)
                     /gene="ysaA"
                     /locus_tag="ACHK49_001211"
     CDS             complement(18758..19231)
                     /gene="ysaA"
                     /locus_tag="ACHK49_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO9261888.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHCWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19333..20181)
                     /gene="yiaJ"
                     /locus_tag="ACHK49_001212"
     CDS             complement(19333..20181)
                     /gene="yiaJ"
                     /locus_tag="ACHK49_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO9261889.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20382..21380
                     /gene="yiaK"
                     /locus_tag="ACHK49_001213"
     CDS             20382..21380
                     /gene="yiaK"
                     /locus_tag="ACHK49_001213"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO9261890.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21392..21859
                     /gene="yiaL"
                     /locus_tag="ACHK49_001214"
     CDS             21392..21859
                     /gene="yiaL"
                     /locus_tag="ACHK49_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO9261891.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            21977..22450
                     /gene="yiaM"
                     /locus_tag="ACHK49_001215"
     CDS             21977..22450
                     /gene="yiaM"
                     /locus_tag="ACHK49_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFO9261892.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            22453..23730
                     /gene="yiaN"
                     /locus_tag="ACHK49_001216"
     CDS             22453..23730
                     /gene="yiaN"
                     /locus_tag="ACHK49_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO9261893.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            23743..24729
                     /gene="yiaO"
                     /locus_tag="ACHK49_001217"
     CDS             23743..24729
                     /gene="yiaO"
                     /locus_tag="ACHK49_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO9261894.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            24733..26229
                     /gene="lyxK"
                     /locus_tag="ACHK49_001218"
     CDS             24733..26229
                     /gene="lyxK"
                     /locus_tag="ACHK49_001218"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO9261895.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHTLPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTVHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            26226..26888
                     /gene="ulaD"
                     /locus_tag="ACHK49_001219"
     CDS             26226..26888
                     /gene="ulaD"
                     /locus_tag="ACHK49_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO9261896.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            26881..27741
                     /gene="sgbU"
                     /locus_tag="ACHK49_001220"
     CDS             26881..27741
                     /gene="sgbU"
                     /locus_tag="ACHK49_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO9261897.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            27735..28430
                     /gene="araD"
                     /locus_tag="ACHK49_001221"
     CDS             27735..28430
                     /gene="araD"
                     /locus_tag="ACHK49_001221"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO9261898.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(28462..28563)
                     /locus_tag="ACHK49_001222"
                     /pseudo
     CDS             complement(28462..28563)
                     /locus_tag="ACHK49_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            28567..28830
                     /locus_tag="ACHK49_001223"
                     /pseudo
     CDS             28567..28830
                     /locus_tag="ACHK49_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28777..29517)
                     /gene="yiaT"
                     /locus_tag="ACHK49_001224"
     CDS             complement(28777..29517)
                     /gene="yiaT"
                     /locus_tag="ACHK49_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO9261899.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITNSPMIDRNESFTFITGVSWRF"
     gene            29641..30615
                     /gene="yiaU"
                     /locus_tag="ACHK49_001225"
     CDS             29641..30615
                     /gene="yiaU"
                     /locus_tag="ACHK49_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9261900.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQQPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(30612..31748)
                     /gene="yiaV"
                     /locus_tag="ACHK49_001226"
     CDS             complement(30612..31748)
                     /gene="yiaV"
                     /locus_tag="ACHK49_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO9261901.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYKRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(31754..32077)
                     /gene="yiaW"
                     /locus_tag="ACHK49_001227"
     CDS             complement(31754..32077)
                     /gene="yiaW"
                     /locus_tag="ACHK49_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO9261902.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
BASE COUNT         7989 a         8240 c         8007 g         7904 t
ORIGIN      
        1 ctgtattatt tagcgataat aaaaaaagcc tgccagatgg caggcttttt aataacggcg
       61 ttattactgc aacagcgaaa tatccgcaac gcgcaggaac agttcgcgca gtttctccag
      121 catggtcaga cggttgagac gcaattcttt gtcatcaacc atgaccatca ctttatcgaa
      181 gaaagcatca accggctcac gcagctctgc cagttcgacc agcgcatcct ggtaacgacc
      241 ttccgcaaag tacggctcca gcttgtcacg tagcaccaca acctgcatcg ccagtttaat
      301 ttcttccggc tctttcaggg tagaggcatt cacgcggtcg ctcagcactt cgtcagattt
      361 cgccaaaatg ttggagacac gcttgttcgc cgccgccagt gcagcagctg cttcaagggt
      421 acggaagtgc gataccgctt tcatgcgggc atcgaaatca gccggacgag tcggacgacg
      481 cgccagtacc gcctggatgg tgtcaacggt gtaaccttcg tcctgatacc aggcgcggaa
      541 gcgaccgagc ataaagtcga taacatcatc aaccacgttg gcgttagtca gcttatcgcc
      601 atacagacgc actgcttctt cggtcagcgt ttgcagatca aggttgaggt tcttctcaac
      661 gataattcgc agcacgccaa gtgcggcacg acgcagcgca aacgggtctt tgtcgccttt
      721 cggatgctgg ccgataccga agatacccgc cagagtatcc atcttgtcag caatcgccag
      781 cgcacaggct accgggttag acggcaggtc atcaccggca aagcgcggct gatactgctc
      841 attcagcgcc accgcgacat cttccgcttc gccatcgtga cgcgcatagt gcatccccat
      901 aacgccctgg gtgtcggtga actcgaagac catgttggtc atcaggtcac acttagacag
      961 caggcccgca cgggttgcgt ggttaacgtc agcgccaatc tgttcagcaa tccagccagc
     1021 cagcgcctgg atgcggtcag ttttgtcgcg cagcgtaccc agctgttgct ggaacagcac
     1081 ggtttgcagg cgcggcaggt tatcttccag acgtttttta cggtcggtat tgaagaagaa
     1141 ctcggcatcc gccagacgtg gacgaacgac tttctcgtta ccagagataa tttgctgcgg
     1201 atctttcgat tcgatgttgg caacgaagat gaagttcggc agcagtttgc catcgttcgc
     1261 atacaccggg aagtatttct ggtcaccttt catggtgtaa accagggctt cagccggcac
     1321 cgcgaggaat ttctcttcga attttgcggt cagaacgacc ggccactcca ccagcgaagc
     1381 cacttcttcc agcaggcttt cgcttaagtc agcgttaccg ccaatcttac gcgctgcttc
     1441 ttcggcatcg gctttaatct tcgctttacg ttcttcgtaa tcggcgatga ctttcccgcg
     1501 ctcacgcaga atttccggat actgatcggc gttgtcgatg gtgaattccg gttcgcccat
     1561 aaagcggtgg ccgcgaatca cgcgatcgga ctgaatgccc agaatggttg ccggaatgac
     1621 tttgtcgccc agcagcaggg tcacggtgtg caccggacgc acgaagtgca cgtcgcttgc
     1681 gccccaacgc atcagtttcg ggatcggcag tttcgccaga gaagtcgcaa ccatattcgg
     1741 cagcagtgct tcggtgcttt cgcccttcac atgggcgcga tacagcagcc attcgccttt
     1801 atcggtagtc agacgctcag cctggtcaac ggtaataccg caaccacgcg cccaaccttc
     1861 tgccgctttg ctcggtttgc cttcagcgtc gaacgcctgg gcaatcgccg ggccgcgttt
     1921 ttcgatttca cgatccggtt gcgcttccgc caggttagct actttcagcg ccagacgacg
     1981 cggagcagca aaccattgaa cggtgccgtg tgcgaggcca gcgttatcca gctccgcagt
     2041 aaaattcgca gcaaaggact cagccaggct gcgcagtgct tttggtggca gctcttcagt
     2101 gccgatttcc accagaaaag ttttctcaga catagccgcc tcttacttat ctttgttgca
     2161 catcgggaag ccgagggctt cacgggaagc gtagtatgct tctgccactg ctttggtcag
     2221 ggtgcgaatg cgcagaatgt agcgctgacg ctcggtgacg gagatggctt tacgcgcatc
     2281 cagcaggttg aagctgtggg cggctttcag aatacgctcg taggctggca gcggcagcgg
     2341 attttccagc gccagcagct gctgcgcttc tttctcgtac tgctcaaagc aggtgaacag
     2401 gaagtccaca tccgcgtatt cgaagttgta agtggactgc tccacttcgt tctgatggaa
     2461 cacgtcgccg taggtggttt tacccagcgg gccgtcgctc cagaccaggt cgtaaacgct
     2521 gtctacgccc tgaatgtaca tggccagacg ttccagaccg taggtgatct cgccggtaac
     2581 cggtttacac tccagaccac caacctgctg gaagtaagtg aactgcgtca cttccatgcc
     2641 gtttagccac acttcccagc ccagtcccca ggcacccaga gtcgggtttt cccagttatc
     2701 ttccacgaaa cggatgtcgt gaatagtcgg gtccatgccc agctctttca gagaaccgag
     2761 gtacagctcc tgaatattgt ccggcgatgg cttaatgacc acctggaact gatagtagtg
     2821 ctgtaaacgg ttggggtttt cgccgtagcg accatcggtc gggcgacgag aaggctgaac
     2881 ataagcagcc gccatcggtt ctggccccag cgcgcgcaga caggtcattg ggtgagaggt
     2941 tcccgcgccg acttccatgt ccaatggttg aacaatggtg cagccctggc gagcccagta
     3001 atcctgtaag gtcaggatca agccctggaa ggtcctggta tcaaactttt gcatattatt
     3061 tcgtgctgga tacgtgtgga tttaaaggaa gggatcagta tacccgctgg atggaagata
     3121 tacagtacga aacgggaaaa agcagggctt aacgcatgga aagatgcaaa aaatggccat
     3181 ctgcgtcaaa agaacagaca aactgctctt tacggaaggt ataaccgcgc atttcatagc
     3241 ttccctggaa ctgttcgaag gcggtgacgt cgattttttg ctcgccggtg ttataacgtt
     3301 ttgccgcctg atccttgcat aacgcttcca tactcaacga acgcagagga tcaaccttta
     3361 cccgttgtgc tttctgcacg ggtgacggtg tagaacaacc cactagcacc ataagcgcca
     3421 ttgccggaaa gaaagcgttc atcatcatta aattaccgtc tggtttgcag gtgagtctta
     3481 ttatttatat cggtatagtg gctgatatct tacgaatttc cttaagcctc tataagctgt
     3541 agtgataaaa ctcagattat tccagtgcgc caaccaaatg atttatcggt cagacttttt
     3601 ttaaccagat acagagacac agatgcagcc caaaatttac tggattgata acctgcgagg
     3661 gatagcgtgt ttaatggtgg tgatgattca caccactacc tggtatgtga ccaatgctca
     3721 tagtgttagc cccgtcacat gggatatcgc caatgttctg aattctgcct ctcgtgtcag
     3781 cgtgccgcta tttttcatga tttccggcta tctctttttt ggcgaacgca gcgcccagcc
     3841 gcgccatttc ttgcgtatcg gcttatgtct gattttttat agcgcaatcg cactgctcta
     3901 cattgcgctg tttacctcca tcaatatgga gttagcgctg aaaaacctgc tgcaaaagcc
     3961 agtgttttac cacttgtggt ttttcttcgc gattgcggtg atttatctgg tttcaccgct
     4021 gattcaggtg aagaacgtcg gcggaaaaat gttgctggtg ctaatggtgg tgattggcat
     4081 tatcgctaac ccaaacacag tgccgcagaa aattgacggt tttgaatggc tgccaattaa
     4141 cttatatatc aatggcgata ctttttacta cattctgtat ggcatgttgg gccgcgctat
     4201 agggatgatg gacacacagc ataaagcact gtcgtgggtg agcgccgcgc tgtttgcgac
     4261 gggggttttt attatctctc gcgggacatt atatgaattg cagtggcgcg gaaattttgc
     4321 cgatacctgg tatctttact gtgggccgat ggtttttatc tgcgcaatcg cgctattgac
     4381 tctggttaaa aacacgctgg atacgcgtac cattcgcgga cttggcttaa tctcccgcca
     4441 ttcattgggt atatacggat tccacgcctt gattatccat gcgctgcgca cccggggaat
     4501 tgagcttaaa aattggccaa tactggatat tatttggatc ttttgcgcga cgttggcagc
     4561 gagtttgtta ctttctatgc tggtacaacg aatcgacaga aacagattag tgagttaagt
     4621 aaaagcccgt tcacattgga ctgaccgggc ttacgtgaat tattcgctgt attcttcctg
     4681 ggtaacctgt gtttcttttg gcgggtttat tccggtatca cgaatattct tctgcaccgc
     4741 tactgcacca aacaagctta agaagaaagc cagtccataa aaaccttttt cactgagtaa
     4801 taatgtcgca ttccacagac ctaccatcag taatgcaaca gagataataa agacagtcag
     4861 gcaggtcata taataaatgg aagtggtcgg tatgccttca tacttgtccc gaacggtctt
     4921 ttgataagac gcagcagaaa acagtcctaa caccagtacg gcaaaataat accctttttc
     4981 atttaactgc atctctgcat tccatagccc taacagatag gtaacgatac caccaacgag
     5041 agctatccat gacacaatac taaaggccgg tgaataggtt gatattttgt tgtccatcac
     5101 agtattcctt tcattcctga atgtgtaaga gctttatgtt gctcataccg agcgaaacaa
     5161 acgcatttga ccaaacaagc agacaaaaaa ggcgatgggg tagatcacca tttcataagc
     5221 attgataggt gcatttaaaa caccgataaa caagagaccg atagtgatta acgcaactaa
     5281 ctggcagata tgggtaaaaa aattatccag ttgcccgagt ttttctgcgc gaagatatgc
     5341 atagttacca aaagttgctg tcattaacac gccaagaaaa tagccttttc cacttagcaa
     5401 tgggcatgag atccatagcc caaccagata aaccagcgcc ccgacaacaa ataatagttt
     5461 tgccacagtt tttgaggttt tcataacgat ctccatatct accagcgata cattacgagt
     5521 aaccaacgaa agacaaaact gaaaaatgcc attaacaaat gattttcaga ataaattcat
     5581 actaaatatt aattaattag tgagatatat agatgtgaat tatcccccac ccggtcaggc
     5641 aggggataac gtttacgcca ttaatggcag aagttgctga tagaggcgac ggaacgtttc
     5701 acgtcgtggc tgataagcgg cataacgctg cgcatctggt agatgcgact gttctaacgg
     5761 tagctgcggc aacaactcaa tgagcgattt ctctggattc gccgcaatct gcgccagcct
     5821 tgctgcgccc agtgctggcc ccacatcccc ccccgtacgg taatcgagct gctgaccgct
     5881 gatatccgcc agcatctgac gccagtactc actacgcgcc ccgccaccaa tcaacgtaac
     5941 actttgcggt ttaatgccgc aggcatgcac gacatccatg ccatctgcca gcgcataacc
     6001 cacgccttcc agcactgctc gcgccagttc attggggcca tgttgatgag tcaaaccaaa
     6061 gaaaaccccc ttcgcctggg gattattgtg tggtgtacgc tcgccggaaa gataaggcag
     6121 aaaccaaact ggctcggcac tttcatcagc ctgttgagct gcagcgatta aagctgggac
     6181 attgcacagg ccggttaatt tcgcgaccca atccagacac gatgctgcac tcagcattac
     6241 agacattaaa tgccaacgct gcggtagcgc atggcaaaag ctgtgtacag cgctttctgg
     6301 cttgcttaag aacccttcgc tgacagcaaa atagaccccc gacgtcccca gcgataacat
     6361 tgcctgatta gcatcaacca ttcccacacc aactgcacca gctgcattgt cgccaccgcc
     6421 tgcgacaact ggcaccgtcg ccatacccca cgctttcgca acttcaggta acaaagcacc
     6481 agtaatttcg ctgccttcgt ataatgcggg catctggtca cgagataagt cgcaagcctg
     6541 cagcatgacg tcactccagt cacgctttgc gacatccagc cacatggtgc cagctgcgtc
     6601 agacatatcg ctggcaaact cccccgtcat acgcagacgc aagtaatctt tcggtaataa
     6661 tactttgtcg atttgacgga atatctccgg ctcatgccgc tgaacccata gcaatttagg
     6721 cgcagtaaat ccgggcatca tcaggttgcc ggtaatcact cgtgattgcg gaactctcgc
     6781 ttccagcaaa gtgcactctt gcgcacagcg cccgtcgttc cacaaaatgg cagggcgcaa
     6841 tacccgttgt tgagcatcca gtaaggttgc tccatgcatc tggccggcaa tacccaatgc
     6901 tttaacgtcc tgcagagaat gctgatcgcc cagagctttc attgcgcgat cagttgcctg
     6961 ccaccactgt tccgggtctt gttccgacca gagtggatgc gggcgcgaaa cggtcagctt
     7021 ttccgtttgc gaagcaacca cctcaccctg ctcgttgagc aaaataactt ttacgcccga
     7081 ggtgccaaga tctatcccga tatacatatc gatcgttcct taaaaaaatg cccggtatcg
     7141 ctaccgataa ccgggccaac ggactgcaca gttagccgtt atttgtcgaa cagataatga
     7201 tttaccagat tttccagttg ctcctggcga ccactctgat gcaccggaga caaattatgt
     7261 tcctgagcat atttggctaa atctgccagt gacatttggc ctttcaggat ttgctggccc
     7321 aattcgctat tccagccgga ataacgctgc gcgatgcgtt tatccagctc gccatcttca
     7381 atcatgcgcg ctgcaatttt cagcgccact gccatcgtat ccatcgcgcc gatatgaccg
     7441 taaaacagat catatttatc agtactttga cgacgtactt tggcatcgaa gttcagacca
     7501 ccggtggtga aaccacctgc tttgagaatt tcatacatca ccagcgcatt ctcttccaca
     7561 ctgttcggga actggtcggt gtcccagccc agttgcgcat cgccacggtt ggcgtcgaca
     7621 gaaccgaaca ggccaagcgc aatggcggtg gctatttcat gatggaaaga gtgacctgcc
     7681 agcgtcgcgt ggttagcttc aatgttcagt ttaatctctt tttccagacc aaactgtttc
     7741 aggaagccat agaccgtcgc ggcatcgtaa tcatattgat gtttagtcgg ttcttgcggt
     7801 ttcggttcga taagcaacgt gccctggaag ccgattttat gtttatgctc aaccaccatc
     7861 tgcataaagc ggcccagttg ttcacgctcc tgacgcaagt cggtatttaa cagcgtttcg
     7921 taaccttcac gaccacccca caggacatag ttttcaccgc ccaatttatg ggttgcttcc
     7981 atcgctgtaa caacttgcgt tgccgcccag ctgaagactt caggatctgg gttcgtcgcc
     8041 gcacccgcgc catagcgagg gtttgtaaag cagttagcgg ttccccacag cagcttcacg
     8101 ccgctctctt cttgcttggc tgccaggaca tcaaccattt gcgcaaaatt attgatgtac
     8161 tcttttaacg acgcgccctc aggggaaaca tccacatcgt ggaagcaata aaatggcaca
     8221 tgtaacttgt ggaaaaactc aaatgcgaca tctgctttac gcttcgccaa cgccagtgcc
     8281 tcaccaggct gctgccacgg acgattaaac gcccccacac caaacatgtc cgccccgttc
     8341 cagcagaagg tgtgccagta gcaggcggca aaacgcaagt gctcttccat acgcttaccc
     8401 aacaccagtt cgtcgggatt gtagtgacgg aatgctaacg ggtttgagga ttttgagcct
     8461 tcataacgaa cgcgatcgag ctggtcaaaa taggcttgca tattgaactc cataatcagg
     8521 taatgccgcg ggtgatggat gatgtcgtaa tattgggcac tccctttcag ttgctcaatt
     8581 atgttatttc acactgctat tgagataatt cacaagtgtg cgctcgctcg caaaataaaa
     8641 tgggatgatg aaactgggta attcctcgaa gataaaaatg caataagtac aattgcgcaa
     8701 caaaagtaag atctcggtca taaatcaaga aataaaccaa aaatcgtaat cgaaagataa
     8761 aaatctgtaa ttgttttccc ctgtttagtt gctaaaaatt ggttacgttt atcgcggtga
     8821 ttgttactta ttaaagctgt cctctaacta cagaaggccc tacaccatga aaataaagaa
     8881 cattctactc accctttgca cctcacttct gcttaccaac gttgcggcac acgccaaaga
     8941 agtcaaaata ggtatggcga ttgatgatct ccgtcttgaa cgctggcaaa aagatcgaga
     9001 tatttttgtg aaaaaggcag aatctctcgg cgcgaaagta tttgtacagt ctgcaaatgg
     9061 caatgaagaa acacaaatgt cgcagattga aaacatgata aaccggggtg ttgatgttct
     9121 tgtcattatt ccgtataacg gtcaggtatt aagtaacgtt gtaaaagaag ccaaacaaga
     9181 aggtattaaa gtattagctt acgatcgtat gattaacgat gcggatatcg atttttatat
     9241 ttctttcgat aacgaaaaag tcggtgaact gcaggcaaaa gccctggtcg atattgttcc
     9301 gcaaggtaat tacttcctga tgggcggctc gccggtagat aacaacgcca agctgttccg
     9361 cgccggacaa atgaaagtgt taaaacctta cgttgattcc ggaaaaatta aagtcgttgg
     9421 tgaccaatgg gttgatggct ggttaccgga aaacgcattg aaaattatgg aaaacgcgct
     9481 aaccgccaat aataacaaaa ttgatgctgt agttgcctca aacgatgcca ccgcaggtgg
     9541 ggcaattcag gcattaagcg cgcaaggttt atcagggaaa gtagcaattt ccggccagga
     9601 tgcggatctc gcaggtatta aacgtattgc tgccggtacg caaactatga cggtgtataa
     9661 acctattacg ttgttggcaa atactgccgc agaaattgcc gttgagttgg gcaatggtca
     9721 ggaaccaaaa gcagatacca cactgaataa tggcctgaaa gatgtgccct cccgcctcct
     9781 gacaccgatc gatgtgaata aaaacaacat caaagacacg gtagttaaag acggattcca
     9841 caaagagagc gagctgtaag cgttacgccc cggcgcggag cgggggcgtg atttctctcc
     9901 atgccgcgtg aatgaattgg cttaggtgga gtcgttatgc cttatctact tgaaatgaag
     9961 aatattacca aaaccttcgg cagtgtgaag gcgattgata acgtctgctt gcggttgaat
    10021 gctggcgaaa tcgtctcact ttgtggggaa aatgggtctg gtaaatcaac gctgatgaaa
    10081 gtgctgtgtg gtatttatcc ccatggctcc tacgaaggcg aaattatttt tgcgggagaa
    10141 gagattcagg cgagtcacat ccgcgatacc gaacgcaaag gtatcgccat cattcaccag
    10201 gaattggccc tggtgaaaga attgaccgtg ctggaaaata tcttcctggg taacgaaata
    10261 acccacaatg gcattatgga ttatgacctg atgacgctac gctgtcagaa gctgctcgca
    10321 caggtcagtt tatccatttc acctgatacc cgcgttggcg atttagggct tgggcaacaa
    10381 caactggttg aaattgccaa ggcacttaat aaacaggtgc gcttgttaat tctcgatgaa
    10441 ccgacagcct cattaactga gcaggaaacg tcggttttac tggatattat tcgcgatcta
    10501 caacagcacg gtatcgcctg tatttatatt tcgcacaaac tcaacgaagt caaagcgatt
    10561 tccgatacga tttgcgttat tcgcgacgga cagcacattg gtacgcgtga tgctgccgga
    10621 atgagtgaag acgatattat caccatgatg gtcgggcgag agttaaccgc gctttaccct
    10681 aatgaaccac ataccaccgg agatgaaata ttacgtattg aacatctgac ggcatggcat
    10741 ccggtcaatc gtcatattaa acgagttaat gatgtctcgt tttccctgaa acgtggcgaa
    10801 atactgggta ttgccggact cgttggtgcc ggacgtaccg agaccattca gtgcctgttt
    10861 ggcgtgtggc ccggacaatg ggaaggaaaa atttatattg atggcaaaca ggtagatatt
    10921 cgtaactgtc agcaagccat cgcccagggg attgcgatgg tacccgaaga cagaaagcgc
    10981 gacggcatcg ttccggtaat ggcggttggt aaaaatatta ccctcgccgc actcaataaa
    11041 tttaccggtg gcattagcca gcttgatgac gcggaagagc aaaaatgtat tctggaatca
    11101 attcagcaac tcaaagttaa aacgtcgtcc cccgatcttg ctattggacg tttgagcggc
    11161 ggcaatcagc aaaaagcgat cctcgctcgc tgtctgttac ttaacccgcg catcctcatt
    11221 cttgatgaac ctaccagggg tatcgatatt ggcgcgaaat acgagatcta caaattaatt
    11281 aaccaactcg tccagcaggg tattgccgtt attgtcatct cttccgaatt acctgaagtg
    11341 ctcggcctta gcgatcgggt actggtgatg catgaaggga aactaaaagc caacctgata
    11401 aatcataacc tgactcagga gcaggtgatg gaagccgcat tgaggagcga acatcatgtc
    11461 gaaaagcaat ccgtctgaag tgaaattggc cgtaccgaca tccggtggct tttccgggct
    11521 gaaatcactg aatttgcagg tcttcgtgat gattgcagct atcatcgcaa tcatgctgtt
    11581 ctttacctgg accaccgatg gtgcctactt aagcgcccgt aacgtctcca acctgttacg
    11641 ccagaccgcg attaccggca tcctcgcggt aggaatggtg ttcgtcataa tttctgctga
    11701 aatcgacctt tccgtcggct caatgatggg gctattaggt ggcgtcgcgg cgatttgtga
    11761 cgtctggtta ggctggcctt tgccacttac catcattgtg acgttggttc tgggactgct
    11821 tctcggtgcc tggaacggat ggtgggtcgc gtaccgcaaa gtcccttcat ttattgtcac
    11881 cctcgcgggc atgttggcat ttcgcggcat actcattggc atcaccaacg gcacgactgt
    11941 ttcccccacc agcgccgcga tgtcacaaat tgggcaaagc tatctacctg ccagcactgg
    12001 cttcatcatt ggcgcgcttg gcttaatggc ttttgttggt tggcaatggc gcggaagaat
    12061 gcgccgtcag gctttgggtt tgcagtctcc ggcctctacc gcagtagtcg gtcgccaggc
    12121 tttaaccgct atcatcgtat taggcgcaat ctggctgttg aatgattacc gtggcgttcc
    12181 cactcctgtt ctgctgctga cattgctgtt actcggcgga atgtttatgg caacgcggac
    12241 ggcatttgga cgacgcattt atgccatcgg cggcaatctg gaagcagcac gtctctccgg
    12301 gattaacgtt gaacgcacca aacttgccgt gttcgcgatt aacggattaa tggtagccat
    12361 cgccggatta atccttagtt ctcgacttgg cgctggttca ccttctgcgg gaaatatcgc
    12421 cgaactggac gcaattgcag catgcgtgat tggcggcacc agcctggctg gcggtgtggg
    12481 aagcgttgcc ggagcagtaa tgggggcatt tatcatggct tcactggata acggcatgag
    12541 tatgatggat gtaccgacct tctggcagta tatcgttaaa ggtgcgattc tgttgctggc
    12601 agtatggatg gactccgcaa ccaaacgccg ttcttgattt tgataaaaat tttctcaaag
    12661 ccggttacgt attaccggtt ttgagttttt gcatgattca gcaggaaaag aaccatgttt
    12721 actaaacgtc accgcatcac attactgttc aatgccaata aagcctatga ccggcaggta
    12781 gtagaaggcg taggggaata tttacaggca tcacaatcag aatgggatat tttcattgaa
    12841 gaagatttcc gcgcccgcat tgataaaatc aaggactggt taggagatgg cgtcattgcc
    12901 gacttcgacg acaaacagat cgagcaagcg ctggctgatg tcgacgtccc cattgttggg
    12961 gttggcggct cgtatcacct tgcagaaagt tacccacccg ttcattacat tgccaccgat
    13021 aactatgcgc tggttgaaag cgcatttttg catttaaaag agaaaggcgt taaccgcttt
    13081 gctttttatg gtcttccgga atcaagcggc aaacgttggg ccactgaacg cgaatatgca
    13141 tttcgtcagc ttgtcgccga agaaaagtat cgcggagtgg tttatcaggg gttagaaacc
    13201 gcgccagaga actggcaaca tgcgcaaaat cggctggcag actggctaca aacgctgcca
    13261 ccgcaaaccg ggattattgc cgttactgac gcccgggcgc ggcatattct gcaagtatgt
    13321 gaacatctac atattcccgt accggaaaaa ttatgcgtga ttggcatcga taacgaagaa
    13381 ctgacccgct atctgtcgcg tgtcgccctt tcttcggtcg ctcagggcgc gcggcaaatg
    13441 ggctatcagg cggcaaaact gttgcatcga ttattagata aagaagaaat gccgctacag
    13501 cgaattttgg tcccaccagt tcgcgtcatt gaacggcgct caacagatta tcgctcgctg
    13561 accgatcccg ccgttattca ggccatgcat tacattcgta atcacgcctg taaagggatt
    13621 aaagtggatc aggtactgga tgcggtcggg atctcgcgct ccaatcttga gaagcgtttt
    13681 aaagaagagg tgggtgaaac catccatgca atgattcatg ccgagaagct ggagaaagcg
    13741 cgcagtctgc tgatttcaac caccttgtcg atcaatgaga tatcgcaaat gtgcggttat
    13801 ccatcgctgc aatatttcta ctctgttttt aaaaaagcat atgacacaac gccaaaagag
    13861 tatcgcgatg taaatagcga ggtcatgttg tagcgatgat gagaattgtc ggcgtcacat
    13921 cagggaactc agcacagcca cctgatgcga gcaaaagcct tatccgaccg acaaatcaga
    13981 taaggctcaa ggaaatgcaa ttacatatgc gccgcgatta accgttggtt atcctggtac
    14041 attgcgaaca ggtagttgtt ataactcttc cctttggtcg agtagccctt cagcttgtga
    14101 atcatcgctg tggcagtcac ttcctgatcc gctttacgca gctgcgcacg cgatttacgg
    14161 aatgaagagt aagccggatg cgtattgagg ttagtgacat aggcgctcac cgattctttg
    14221 acagaactaa actgtgagta ccctttcact ttacccggcg cattggtaca acgtcctttc
    14281 atgcatttca tgccgaacag attgttgttg ttgcgcgcca gctttgacgt tccccaaccg
    14341 ctttctgctg cagccatcgt cgccaccata ctggtgggga taatgtctac gcgttcaagc
    14401 aaggtattcc acgggatttt tcgcgtatta ccagaccact tcaccttgta gcgtttggcg
    14461 atgtctttca gacgcgcacg ctcagcaggt gaccattggc cctgatactg ttttgaaatc
    14521 agccagttac gttccgcagt aatggctgca ttttggctgg taatgtaagg cattacggtc
    14581 cggagaaacg cctttttcct tggtgttccg gaagggtatt ttcgcaaatc aggaagtgaa
    14641 ttactctttg cactattgcg agaatactct tgtttactgc ttacctgttt attactagct
    14701 ttagttaagt gggacttttg actcgctgtt gttgtgtgcg tcttcgctaa cacctcactc
    14761 gaaaacacca gagtgagtaa cataagaatc atcgccccat atcgtcgtat gggagtcaaa
    14821 atcatcaggt ctcctggtcg gatttaatca ttccaacacc ttatattttt cacaaatttg
    14881 agagttgaat ctcaaatcat atcaaaaata gctgtcaaga gcaccccaag gaatagtcca
    14941 aatctgaaac tatgtcacga gttaacgatt cagattggcg ctaaatcgca gaaaatgtgg
    15001 ggtttatcgc aaaattcagc cgttttttgc gcgagatcgc tcacccttgc ttctcatcct
    15061 gtggacttac cgctcaggga tgagttttgt ttggcttatc gctggcaaac tgtctgaaat
    15121 cgcagcaata aggactcatc cgccatgaaa ctcgccgcct gttttctgac actccttcct
    15181 ggcttcgccg ttgccgccag ctggacttct ccggggttcc ctgcctttag tgaacaggga
    15241 acggaaacat ttgtcagcca cgcgcagttg cccaaaggta cgcgtccact aacgctaaat
    15301 tttgaccaac agtgctggca gcctgcggat gcgataaaac tcaatcagat gctttccctg
    15361 caaccttgta gcaacacgca gcctcaatgg cgattgttca gggacggcga atatacgctg
    15421 caaatagaca cccgctccgg cacgccaaca ttgatgattt ccatccagaa cgccgccgaa
    15481 ccggtagcaa gccaggtccg tgtatgcccg aaatgggatg gattaccgct cacgctggat
    15541 gtcagcgcca ctttcccgga aggagccgcc gtacgggatt attacagcca gcaaattgcg
    15601 atagtgaaga acggtcaaat aacgttacaa cccgctgcca ccagcaacgg tttactcctg
    15661 ctggaacggg cagaaactga cgcctctgcc cctttcgact ggcataacgc cacggtttac
    15721 tttgtgctga cagatcgttt cgaaaacggc gatcccagta atgaccagag ttacggacgt
    15781 cataaagacg gtatggcgga aattggcact tttcacggcg gcgatttacg cggcctgacc
    15841 aacaaactgg attacctcca gcagttgggc gttaatgctt tatggataag cgccccattt
    15901 gagcaaattc acggctgggt cggcggcggt acaaaaggcg atttcccgca ttatgcctac
    15961 cacggttatt acacacagga ctggacgaat cttgatgcca atatgggcaa cgaagccgat
    16021 ctacggacgc tggttgatag cgcacatcag cgcggtattc gtattctctt tgatgtcgtg
    16081 atgaaccaca ccggctatgc cacgctggcg gatatgcagg agtatcagtt tggcgcgtta
    16141 tatctttctg gtgacgaggt gaaaaaaacg ctgggtgaac gctggagcga ctggaaacct
    16201 gccgccgggc aaacctggca tagctttaac gattacatta atttcagcga caaaacaggc
    16261 tgggataaat ggtggggaaa aaactggatc cgtaccgata tcggcgatta cgacaatcct
    16321 ggattcgacg atctcaccat gtcgctagcc tttttgccgg atatcaaaac cgaatcaact
    16381 accgcttctg gtctgccggt gttctataaa aacaaaacgg atacccacgc taaagccatc
    16441 gacggcttta cccctcgcga ttacttaacc cactggttaa gtcagtgggt ccgcgactat
    16501 gggattgatg gttttcgggt cgataccgcc aaacatgttg agttgcccgc ctggcagcaa
    16561 ctgaaaaccg aagccagcgc cgcgcttcgc gaatggaaaa aagctaaccc cgacaaagca
    16621 ttagatgaca aacctttctg gatgaccggt gaagcctggg gccacggcgt gatgcaaagt
    16681 gactactatc gccacggctt cgatgcgatg atcaatttcg attatcagga gcaggcggcg
    16741 aaagctgtcg attgtattgc gcagatggat atgacctggc agcaaatggc ggagaaattg
    16801 cagggtttca acgtgttgag ctacctctcg tcgcatgata cccgtctgtt ccgtgaaggg
    16861 ggcgacaaag cagcagagtt attactatta gcgccaggcg cggtacaaat cttttatggc
    16921 gatgaatcct cgcgtccgtt cggtcctaca ggttctgatc cgctgcaagg tacacgttcg
    16981 gatatgaact ggcaggatgt tagcggtaaa tctgccgcca acgtcgcgca ctggcagaaa
    17041 atcagccagt tccgcgcccg ccatcccgca attggcgcgg gcaaacaaac gacactttcg
    17101 ctgaagcagg gctacggctt tgttcgtgag catggcgacg ataaagtgct ggtcatctgg
    17161 gctgggcaac agtgactttt ccggcttccc gttcgtcagt acctcgggaa gccgccaacc
    17221 aggataaaat gtcagcccta atcagcgttg caggataaag caccgctcac tcttcaacag
    17281 accgatttgc accccagcaa atgtagcgtt attgttacct tccttgctac agagttcgac
    17341 agatatcccg ctatgacatt ctcccttttt ggtgacaaat ttacccgcca ctccggcatt
    17401 acgctgctga tggaagatct gaacgacggt ttacgcaccc ctggcgcgat tatgctcggc
    17461 ggcggtaatc cggcgcagat cccggaaatg caggactact tccagacgct actgaccgac
    17521 atgctggaaa gtggcaaagc gactgatgca ctgtgcaatt acgacggtcc acaggggaaa
    17581 acggagctac tcacactgct tgccggaatg ctgcgcgaga agttgggttg ggatatcgaa
    17641 ccacagaata ttgcactaac aaacggcagc cagagcgcgt ttttctactt atttaatctg
    17701 tttgccggac gccgtgccga tggtcgggtc aaaaaagtgc tgttcccgct ggcaccggaa
    17761 tacattggct acgctgacgc cggactggaa gaagatctgt ttgtctctgc gcgtccgaat
    17821 attgaactgc tgccggaagg ccagtttaaa taccacgtcg attttgagca tctgcatatt
    17881 ggcgaagaaa ccgggatgat ttgcgtctcc cggccgacga atccaacagg caatgtgatt
    17941 actgacgaag agttgctgaa gcttgacgcg ctggcgaatc aacacggcat tccgctggtg
    18001 attgataacg cttatggcgt cccgttcccg ggtatcatct tcagtgaagc gcgcccgcta
    18061 tggaatccga atatcgtgct gtgcatgagt ctttccaagc tggggctgcc tggctcccgc
    18121 tgcggcatta tcatcgccaa tgaaaaaatc atcaccgcca tcaccaatat gaacggcatt
    18181 atcagcctgg cacctggcgg tattggtccg gcgatgatgt gtgaaatgat taagcgtaac
    18241 gatctgctgc gcctgtctga aacagtcatc aaaccgtttt actaccagcg tgttcaggaa
    18301 actatcgcca tcattcgccg ctatttaccg gaaaatcgct gcctgattca taaaccggaa
    18361 ggagccattt tcctctggct atggtttaag gatttgccca ttacgaccga gcagctctat
    18421 cagcgcctga aagcacgcgg cgtgctgatg gtgccggggc acaacttctt cccagggctg
    18481 gataaaccgt ggccgcatac gcatcaatgt atgcgcatga actacgtacc agagccggag
    18541 aaaattgagg cgggggtgaa gattctggcg gaagagatag aaagagcctg ggctgaaagt
    18601 cactaaaaat tgcctgatgc gctacgctta tcaggcctac atgatttctg caatatattg
    18661 aatttggaag aatttgtagg ccggataagg cgtttacgcc gcatctggca taaacaaagc
    18721 gcactattcc ggcgttgaga aacgccggaa aaacgtttca aacaggctgc tgccgtagcc
    18781 gatgccgctg cactttctcg acatccatgc actgcaacgc ctttgtcggg caggcttcaa
    18841 cacaggccgg tcccgtctcc cgatgccagc acagatcgca cttaatcgcc ctcgcctttt
    18901 tgcgcgaaga aacgacctcc atcgcaccaa acgggcaagc cagcatacag cttttacagc
    18961 caatgcaacg tgtttgttca acgaaaatat gcccatgttc gcggcttatc gcgtcaacag
    19021 ggcagacatt cgcgcacggt gcatcttcac actgatgaca ggctactgcc gtggtccagc
    19081 agtggtcttt aatgacacga atacgggaaa taaactcgtc tggtgacaac gcagcgcaat
    19141 cctgattctc ctgatgcgac actgcgcaag ccacttcaca ggtacggcaa ccgatacatt
    19201 tcgtcgcatc cgcaataata aaccggttca tgatcactcc tttgcactaa aacagcagag
    19261 tgccagagag cgcgtcccta ctgctttgat ccaacgaagg aaatgttaaa gttttggcac
    19321 ttgtccaaag cgttatgtga tcgcgcccag gtcatccctg acagtaaatc ccagttcatt
    19381 agaaatagcc tgcgcggttt cacgcagtgg tttcaggaga tttttctctc ccacctgttt
    19441 cagacgtgat gtcgaaagcg aaatcgacac ggcgtacggc acccgcccat gaatatcaaa
    19501 caccggaaca gcaatacagg agacgccgag ttcgttttct tctctgtcca tcgccgctcc
    19561 gctttcacga atgtgcgcca gttcgtcgaa catcgcgggc agctcggtaa tggtattgcg
    19621 ggttaacggc tggatctcat gctgatggtt ttcccagtat gacttcacgt agtccgggtg
    19681 accaaacgcc atgtagatct tgcccattgc ggaacagtag agcggcatat gctggccaat
    19741 ataggcacgg gttcgcagca tcccggttgt gggttccagc ttataaatca aaatagcgtg
    19801 atcgtcttcg cggctggaga agttaatggt ttcaccagtg gcgatgttca gtgcctcaag
    19861 atgcggagcg gcgatatgaa tgatattcag cgaagacagc gccttctgcc cgacggcaat
    19921 aaatttggta gtcaggcggt aactccctgc ggcgggcgcg gtggtcacat agccgcagga
    19981 ctgtaatccc tgcaataagc gatggacggt actcttattt aaaccagcca gctccgaaag
    20041 atgcgccaac ggacaaccgt ttggatagtt gctcaaaatc tcaatcagca tcaacccgcg
    20101 aaacagactc tggcttccgg ctggacgctc tttttcctgc gccatctcgt tctctttttt
    20161 tcccatcact tctttcccca ttttgtcgcg tcctgatggt agcgcaaagt gtgccgtagt
    20221 tcacgatctc gacagataat ttataaccac ttgattttta tgtcttttga aattcatcaa
    20281 tcagattgct ttgttaaaaa gtgatcgata tatttgaaat caagtttcgc atattgaaat
    20341 tttaagccaa aaaagcgatc aaaaaaacaa ggaagcctgg gatgaaagtg acatttgagc
    20401 agttaaaagc agcctttaat cgggtcttaa tttcacgcgg cgttgacagc gaaacggctg
    20461 acgcctgtgc agagatgttc gcccgcacca ccgaatccgg cgtttattct cacggcgtta
    20521 atcgtttccc tcgtttcatt caacaactgg aaaacggcga tatcattcct gatgcccaac
    20581 ccaaacgtat aaccagcctc ggcgcaattg aacagtggga tgcccagcgt tcgatcggca
    20641 acctgacggc gaaaaagatg atggatcgcg ccattgaact ggctgccgat cacggtattg
    20701 gtctggtggc acttcgtaat gccaaccact ggatgcgcgg cggcagctac ggctggcagg
    20761 cggcggaaaa aggctatatt ggcatttgct ggaccaactc catcgccgta atgccgccgt
    20821 ggggcgcaaa agagtgtcgc attggcacta acccgctgat cgtcgccatt ccttccacgc
    20881 cgatcaccat ggtcgatatg tcgatgtcga tgttctctta cggcatgtta gaagttaacc
    20941 gcctggcggg ccgtcagctc ccggtcgatg gtggctttga tgatgagggc aatttgacca
    21001 aagaacctgg cgttatcgag aagaatcgcc gcattttgcc gatgggctac tggaaaggtt
    21061 ctggcatgtc gattgtgctg gatatgatcg ctactctcct ttccgacggc gcatcggttg
    21121 ccgaagtcac ccaggacaac agcgacgaat acggcatttc acaaattttt attgccattg
    21181 aagtggacaa gcttatcgac ggtcccaccc gcgatgccaa gctgcaacgt atcatggatt
    21241 acgttactac cgctgaacgc gctgacgaaa accaggccat ccgcttaccc ggccacgaat
    21301 ttactaccct gctggccgaa aaccgccgta acggtattac cgttgatgac agcgtgtggg
    21361 ccaaaatcca ggcgttataa ggagataagt catgattttt ggacatatcg cgcagcccaa
    21421 tccgtgccgt ttgcccgccg ccattgaaaa ggcgctcgat tttctgcgcg ctaccgattt
    21481 caacgccctg gagccgggcg ttgtcgaaat cgacggcaaa aatatttatg cgcaaattat
    21541 cgatttaacc acacgcgaag cggtggaaaa ccgtccggaa gtgcatcgtc ggtatatcga
    21601 tattcagttt ctggcgtggg gtgaagagaa aatcggcatt gctattgata cgggaaataa
    21661 taaagtcagc gaatcactat tagagcagcg cgatattatt ttttatcatg acagtgaaca
    21721 tgaatcattt atagaaatga taccgggcag ctacgcaatt ttctttccgc aggatgttca
    21781 ccgaccaggt tgtattttgc aaacagcctc tgagatacga aaaattgtgg ttaaagtcgc
    21841 attaacggcg ctgaattaat cgaataatcg tctacgccag aacgactggg taatgttatt
    21901 gctctaacta tattaataat ttaggttgct gcacatgcaa cttgaattat gacagacata
    21961 gcctcaagga atagctatga aaaaaatact tgaagcaata ctggcgatta atctcgccgt
    22021 actttcctgt attgtgttta taaacattat tttaagatat ggttttcaga caagcatttt
    22081 atctgtagat gaattgtcac gttatttatt tgtctggtta acatttattg gcgcgattgt
    22141 ggcttttatg gataacgccc acgttcaggt cacctttctg gtggagaaac tctcccccgc
    22201 atggcaacga cgagtcgctc tggttaccca ttccttaatc ttatttattt gtggcgcact
    22261 ggcctggggc gcaacgctaa aaacaattca ggactggagc gattattcac cgatcctcgg
    22321 tttacccatc ggcctgatgt atgccgcttg tctgcccacc agcctcgtta tcgcattctt
    22381 tgaactgcgt catttatatc aactcatcac gcgtagcaat tctctaacgt cgccaccgca
    22441 aggagcttaa tcatggctgt gctgattttt ctgggctgtc tgttgggtgg tattgctatc
    22501 ggtttgccta ttgcctgggc actgttgttg tgcggggcgg cgttaatgtt ctggctggac
    22561 atgtttgatg tccagatcat ggcacaaacg ctggtgaacg gagccgatag cttctccctg
    22621 ctggcgattc cgttctttgt gctggcgggt gaaatcatga atgcgggcgg cttgtcaaaa
    22681 cgcattgttg acctgccgat gaaactggtg gggcataaac caggcgggct gggctacgtc
    22741 ggcgtgctgg cagctatgat tatggcgagc ctttctggtt ctgccgttgc ggataccgcc
    22801 gccgttgccg ctctactggt gccgatgatg cgcagcgcca actacccggt caaccgggcg
    22861 gcggggctga ttgcctctgg cggcattatc gcgccaatta ttcccccttc cattccgttt
    22921 attatcttcg gcgtttccag cggattatcc atcagtaagc tgtttatggc gggcattgcc
    22981 cctggcatga tgatgggcgc aacgctgatg cttacctggt ggtggcaggc cagtcgcctt
    23041 aatttacccc gccagcaaaa agcaacgatg caggaaatct ggcactcttt tgtctccggt
    23101 atctgggcgc tgtttcttcc agtcatcatt attggcgggt ttcgctccgg tctgtttacc
    23161 cctaccgaag ccggggccgt tgctgctttc tatgcgctgt ttgtcgccac agttatttac
    23221 cgtgaaatga ccttcgccac actttggcat gtactcattg gcgcggcgaa aaccacctca
    23281 gtggttatgt ttctggtggc ctcggcacaa gtttccgcct ggctgattac cattgctgaa
    23341 ctgccgatga tggtttctga tttactgcaa ccgttggtcg attcaccgcg tctgctgttt
    23401 atcgtcatta tggtggctat cctgattgtc ggcatggtca tggatttaac gccgacggta
    23461 ttaattctta ccccggtatt aatgccttta gttaaagagg caggaatcga tccgatttat
    23521 ttcggtgtca tgtttatcat taactgctca atcggcttaa ttacaccgcc tatcggtaat
    23581 gtacttaacg ttatttcagg ggtggcaaaa ctcaaattcg atgatgcagt cagaggcgtt
    23641 ttcccttacg tcctggtttt atattcatta ttagtcgtat ttgtttttat tcccgatctc
    23701 atcatcctcc ctttgaaatg gattaattaa aaggaaaata ttatgaaatt acgctctgta
    23761 acctacgcat tattcattgc tggcctggct gcattcagca catcttctct ggcggcgcaa
    23821 tctttacgtt tcggttatga aacctcacaa accgactcgc aacatattgc ggcgaaaaaa
    23881 ttcaatgatt tattgcagga gagaaccaaa ggcgagctga aattaaaatt gtttccggat
    23941 agcaccctcg gtaacgcgca ggcgatgatc agcggcgtac gtggcggcac catcgatatg
    24001 gaaatgtccg gctcgaataa ctttgccgga ttatcaccag tgatgaactt gcttgatgtc
    24061 cctttcctgt tccgcgatac cgctcacgcg cataaaacgc tcgacggcaa agtcggtgat
    24121 gatctgaaag cctcacttga aggtaaagga ctaaaagtac tggcctactg ggaaaacggc
    24181 tggcgcgatg tcaccaactc gcgcgcaccg gttaaaaccc ccgccgacct gaaagggctg
    24241 aaaattcgca ccaacaatag cccgatgaat atcgccgcat tcaaagtctt tggcgctaac
    24301 ccgatcccga tgccgtttgc cgaagtctat accgggctgg aaacccgcac tatcgacgct
    24361 caggaacacc cgatcaacgt cgtctggtca gcaaaatttt tcgaagtgca gaagtacctt
    24421 tctctgacgc accacgccta ttccccgctt ctggtggtga tcaacaaagc gaagtttgat
    24481 ggcttaagcc cggagttcca gcaagcgcta atttcatctg cgcaggaagc gggtaactac
    24541 cagcgcaaac tggttgccga agatcagcaa aaaatcatcg acggcatgaa agaagcgggc
    24601 gtggaagtca tcaccgatct cgaccgcaaa gcctttagcg acgcactggg tactcaggtc
    24661 cgcgacatgt ttgtaaaaga tgttccgcag ggtgctgatc tactgaaagc cgtggatgag
    24721 gtgcaataaa ccatgacgca atactggctg gggttagatt gtggcggtag ctggctgaaa
    24781 gccgggctgt atgaccgcga aggccgggag gcaggcgtgc agcgcctgcc gctgtgcgca
    24841 ttaagcccgc agccaggttg ggcagagcgc gatatggcag aactgtggca atgctgcatg
    24901 gctgtcattc gcgaactgct tacccattcc ggcgttagcg gggaacaaat tgtcggtatc
    24961 ggtatctccg cacaaggaaa aggcttgttt ttgctggata aaaacaacaa gccgctcggt
    25021 aacgccattt tatcctcgga tcgccgggcg atggaaatcg ttcgtcgctg gcaggaagat
    25081 ggcatcccgg aaaaactcta cccgctgacc cgacaaacct tgtggaccgg gcatccggta
    25141 tcgctgttac gttggcttaa agagcacgaa ccggaacgct acgcgcaaat tggctgcgtg
    25201 atgatgacgc acgactacct gcgctggtgt ttaaccggcg tcaaaggctg tgaagagagc
    25261 aatatttccg agtccaatct ctacaacatg agtcttgggg aatatgaccc gtgcctcacc
    25321 gactggctgg ggattgctga aatcaatcac accctgccgc ctgttgtcgg atctgccgaa
    25381 atctgcgggg agatcaccgc tcagacagcc gtactgaccg gtctgaaagc gggtacgccc
    25441 gttgtcggcg gcctgtttga tgtggtttcc accgcactct gcgccgggat cgaagacgaa
    25501 tttaccctta atgcggtgat gggcacctgg gcggtgacca gcggcataac ccgcggttta
    25561 cgtgacggtg aagcgcatcc gtatgtctat ggtcgctacg ttaacgatgg tgaatttatc
    25621 gttcacgaag ccagccccac ctcttccggc aacctcgaat ggtttaccgc acagtgggga
    25681 gaaatctcgt ttgatgagat caaccaggcc gttgccagct tgccgaaggc cggtggcgat
    25741 ctctttttcc tgccgttcct gtacggcagc aacgccggac tcgagatgac cagcggtttc
    25801 tacgggatgc aggccattca cacccgcgca cacctgttgc aggccattta tgaaggggtg
    25861 gtgttcagcc atatgaccca tctcaaccgg atgcgcgaac gttttactga tgtgcacacc
    25921 ctgcgcgtca ctggcggccc ggcgcattcc gatgtctgga tgcaaatgct ggcggacgtc
    25981 agcggtctgc gtattgagct gccgcaggtg gaagaaaccg gctgctttgg tgcagcactt
    26041 gccgcccgcg tcggcaccgg ggtttatcgc gatttcagcg aagcccagcg tgatttacag
    26101 cacccggtgc gcaccctgct gccggatatg actgtccatc agctttacca acaaaaatac
    26161 caacgctatc agcatctcat tgccgcactt cagggctttc acgcccgcat caaggagcac
    26221 atattatgag ccgaccactt ctgcaactgg cactcgacca ctcatcactt gaagccgcac
    26281 agcgcgacgt gacgcagtta aaagacagcg tcgatattgt cgaagcgggc accattctct
    26341 gtttaaacga agggcttggc gcggtgaaag ccttgcgcga acagtgccct gacaaaatca
    26401 tcgttgccga ctggaaggtc gccgacgctg gtgaaacgct cgcgcaacag gcatttggcg
    26461 caggcgctaa ctggatgacc atcatctgcg ccgcaccgct cgcgacggtg gaaaaaggcc
    26521 acgcgatggc gcaacgctgc ggtggtgaaa ttcagatcga gctgttcggt aactggacgc
    26581 tggacgacgc ccgcgactgg catcgtattg gcgtgcggca ggcgatttat catcgcggtc
    26641 gtgatgcaca ggccagcggg caacagtggg gcgaagccga tctggcacgc atgaaggcgc
    26701 tttccgatat cggccttgag ctttccatta ccggcgggat tacccctgct gacctgccgc
    26761 tgtttaaaga tattcgcgtg aaagcgttta ttgccgggcg agcactggca ggctcagcaa
    26821 atccggcgca ggtggctggc gatttccatg cgcaaatcga cgctatctgg ggaggcaagc
    26881 gtgcgtaatc atccgttagg gatttatgaa aaggcgctgg cgaaagatct ctcctggccg
    26941 gagcggctgg tgctggcaaa aagctgcggt tttgattttg tcgaaatgtc ggtggatgaa
    27001 accgacgaac ggctttcacg tcttgactgg agcaccgcac aaaggacttc gctggttgcc
    27061 gcgatgatcg aaacgggtgt tgggatcccg tcgatgtgcc tgtccgcaca tcgtcgcttt
    27121 ccttttggta gccgtgacga cgcggtacgc cagcgagcac gggagatcat gagcaaagcg
    27181 attcgtctgg cacgcgatct cggcatacgc accatccagc tggcgggcta cgacgtctat
    27241 tacgaagacc acgatgaagg cacccggcaa cgttttgctg aagggctggc gtgggcggtc
    27301 gaacaggcgg cggcatcaca agtcatgcta gcggtggaga ttatggacac cgcgtttatg
    27361 aactccatca gtaaatggaa aaagtgggac gagatgctcg cctcgccgtg gttcaccgtc
    27421 tacccggacg tcggcaacct cagcgcctgg ggcaatgatg ttcccgccga actgaaactg
    27481 ggcattgacc gtatcgccgc gatccacctg aaagacaccc agccagttac cgagcaaagt
    27541 cccggacagt tccgcgacgt gccgtttggc gaaggctgcg tcgatttcgt tggcatcttc
    27601 aaaacgctgc ataaactgaa ctatcgcggt tctttcctga ttgagatgtg gaccgaaaaa
    27661 gccaaagagc cggtgctgga gattattcag gcgcggcgtt ggattgaagc gcgtatgcag
    27721 gaggctggat ttatatgtta gagcaactga aagccgacgt gctggcggcg aatctggcgc
    27781 ttcccgctca ccatctggtg acgttcacct ggggcaatgt cagcgcggta gacgaaacgc
    27841 ggcaatggat ggtaatcaaa ccttccggcg tcgagtatga cgtgatgacc gccgacgata
    27901 tggtggtggt tgagatagcc agcggcaagg tggtggaagg tagcaaaaag ccctcgtccg
    27961 acacgccaac gcatctggcg ctttaccgtc gctacgccga aatcggtggc attgtgcata
    28021 cccactcacg ccacgccacc atctggtcgc aggccgggct ggatctcccc gcctggggca
    28081 ccacgcacgc tgattatttc tatggtgcca tcccctgcac gcgacagatg accgcagagg
    28141 agattaacgg cgaatatgaa tatcagaccg gcgaagtgat cattgaaacc ttcgaagaac
    28201 gtggcaggag tccggcacaa atcccggcgg tgctggtgca ttctcacggc ccgttcgcat
    28261 ggggtaaaaa cgccgccgat gccgtgcata acgccgtagt actcgaagaa tgcgcctata
    28321 tgggcctatt ctcgcgccag cttgcgccgc agctccctgc gatgcaaaac gaactgctgg
    28381 ataagcacta cctgcgtaag catggggcca atgcctatta cgggcagtaa tccctcacgc
    28441 cggggcttca tcgccccggc actacgaatt gatatgttcc ttgctgtaac gccgcttcca
    28501 cgctgctggc gttaaaccgg tgtgcttgcg aaaaatctgc cggaaatagc caacgtcatt
    28561 aaaccgtatt gcatggagca ggccgacaac gtcgagtcac tgcataacct gttgctgccc
    28621 gccgatgcgc catttacgac atttgaaggc aagggattgt tcagccataa gatcctgatt
    28681 cagacgccag gttcccggta tgaacagagc aatccagagc aacaaccttt gtggcattac
    28741 gacggagcac cagccgcacc gacatccacc ggtgaattaa aaacgccagc tcacgccggt
    28801 gataaaggta aaactctcat tacgatcaat catcgggctg ttggtaatct cttccggcag
    28861 ctcgctgtat cctgcgctcg ccatcaatac gacgtgctct cctatcgggt attttgccgt
    28921 caggctgaca tagggcaccc aggcatcctg cgcggaataa cttgccagac cgctacgacg
    28981 ggactcactc tctgaaatgc catagtaata gtcactgaaa ttctcgtcat aatagagaac
    29041 gcccagcgca ggtgtcagcg acaggcgacc tatctgcatt ttgtggaata ccgatagctc
    29101 ccccacccag ccgttgctgt tatccagaac gtccgcagcc gcagaggctt tcacggttcc
    29161 ccaacgctcg tggtgatacc aggcaacccc cgccatagcc gtactatcac gcttatcaag
    29221 ctgttgcatg gcataatcgt cattatcggc agggtcaaat tccagcggca tccaggatgc
    29281 ggtcaggcta agttcatttt tttcactttg cgacagaata aaacctaatg tggtctgacg
    29341 aacataaaaa gaatcacctt cataactaat cagcggaatt gcctttgtat tttcattata
    29401 accgcgataa ggcgattcat tataagctgc accagcacca atggataatt cagaagcagt
    29461 tgcgcttgcc ataaaaggca acgcaaataa cgccacaata ttgcgattaa ttaacatgtc
    29521 cggtattcca ttttaaaata agataaaaaa gagtcggcat aataatgctt acaatttctc
    29581 cggtgcaaga ttcatattta tatagtaata tataaaatta tatataattg ggctgttgcg
    29641 atgacaaaat tacaacttaa gtaccgggag ttaaaaatta tctcggtaat cgctgccagt
    29701 gaaaatatca gccatgccgc gactgtactt ggcatcgcac aggccaacgt cagcaaatat
    29761 cttgctgatt ttgaatcaaa agtgggttta aaagtttttg accgaaccac ccggcagctg
    29821 atgctcacac cttttggcac cgcgctgtta ccgtacatca acgacatgct ggacagaaac
    29881 gagcaactta ataattttat tgcagattat aagcatgaga aacgtggtcg ggtcaccatc
    29941 tacgccccaa ccggcataat tacctattta tccaaacatg taatcgataa aattaaagat
    30001 atcggtgata tcaccttatc gctaaaaacc tgcaatcttg agcgtaatgc cttttatgaa
    30061 ggcgtcgaat ttcctgatga ttgtgatgtt ttaatcagct acgctccacc aaaagatgaa
    30121 tcgctggttg ccagttttat tacccaatat gccgtaaccg cttatgccag ccagcgctat
    30181 cttgagaaac atcccattag ccgtcctgac gaactcgaac atcactcctg tattttgatc
    30241 gactcgatga tgatcgacga cgcgaatatc tggcgcttca acgtcgccgg gagtaaagag
    30301 gtgcgggatt atcgcgtgaa gggaaattac gtttgcgaca acacgcaatc tgcgctggag
    30361 ctggcacgaa atcacctggg gattgtgttt gcgccagaca aaagtgtgca gagcgacctg
    30421 caagacggca cgctggtgcc ctgctttcag caaccctatg aatggtggct ggatctggtg
    30481 gctatcttcc gtaaacggga ataccagccc tggcgggtcc agtatgtgct ggatgaaatg
    30541 ctgcgtgaaa ttcgccacca gcttgctcag tcgcagcaac tgcggcctga acaagcggcg
    30601 gaaagcgaag attaatgatc gagataaagg taatgcaccc agctggtcat gcgtaatagc
    30661 actttgcgca tcaccgagac atggctgaaa tgatcagagt aaaccgccac ctgggcgtaa
    30721 ataccgtctg gtaaagcgct caaatcagtg tgctcatcca gttcaatggt cgcgataacg
    30781 ccatctgaac ccggcgctgt gtttaacgtc tgtaaggtgc cggtcgactg ataagctccg
    30841 ccgggaacgg ctggactaat ggctgccagc ttaccgctga ataccttacc tggcagagca
    30901 ttaaacacca cttccgcatc gtcgccagga gccagtcgca gcaaggagtt ctgacggaac
    30961 tgcgccacga tttgtcgttt ctgatcgggt ataaacacca tcaccggacg tagcggcagc
    31021 gacgcggcat aggtacccgg acgaatcagt acctgggtca cataaccatc gctcggcgca
    31081 cgcactatcg tctgctcaag gttatatttt gcttccgcga gctgtgcttt caggctggcg
    31141 atttgagaat gttcacccaa caccaggcta tccagctggc tctggatctg tttttgttcc
    31201 gccgccgatg acttcacgga ggcttcctgc gccagataat tttgccgcgc cacatcgata
    31261 tcgcgttctg aaaacgggtt tactttcgcc tggctgccac gtgcgtaacg cttatactct
    31321 ttagcgaatt tatcccgcgt ggcctttgcc tgctgagtat tcgccgccat ctcatctaac
    31381 tctgcgccca acgcccgctg tttatgttct gcggtaacga tatccgccat cagccgatcc
    31441 acccgcgcct gataacgcgt cgggtccagt cgaaatagca cctcaccttt tttaatcagc
    31501 gtatttttct tatccgtcac ttcgatcacc acgcctgtca cctgtgggac aacaggaata
    31561 gaaataaccg ctttttgcgc tttgaaggta tacggatggt tatagttcat taacagaatt
    31621 aaaccactga caataaatat gccacccagg gccgctgtgg gaatggtcca tttatttaca
    31681 ggaattttga agatcttaaa cattgcccat gcaaaagcca cataggtcaa aataatcagt
    31741 aaatccataa gtattactcg gcagaagagg atttgatgtc ggcgagctgt ttctccagcc
    31801 cggcaatacg ctgttgtagt tgcacaacgg aggagtcatg gttttgcatt ccccatcccc
    31861 gctccgggcg gtaaagcgtg gcccaaatcc acaaaaatgg ccagataaca tgcagcgtaa
    31921 aaagactcac ccaaccagca acatgaatgg cgtcggcatg aggatgatta cgttttttag
    31981 caatcaggta gggaatatca tgtaaaataa taatcccata gaatatcacc agaaatacaa
    32041 aaataagcac tcccagtgca aaataatcca gaaacatatt tccctcagag aatataaaaa
    32101 agaacaatta acgcatatta tgaaatccca tgatgcagtg
//
LOCUS       DAZGLJ010000028        31575 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000028 DAZGLJ010000000
VERSION     DAZGLJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.028"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(6..893)
                     /gene="rarD"
                     /locus_tag="ACHK49_001228"
     CDS             complement(6..893)
                     /gene="rarD"
                     /locus_tag="ACHK49_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO9261903.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(945..1412)
                     /gene="yigI"
                     /locus_tag="ACHK49_001229"
     CDS             complement(945..1412)
                     /gene="yigI"
                     /locus_tag="ACHK49_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO9261904.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1577..2446
                     /gene="pldA"
                     /locus_tag="ACHK49_001230"
     CDS             1577..2446
                     /gene="pldA"
                     /locus_tag="ACHK49_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO9261905.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2579..4408
                     /gene="recQ"
                     /locus_tag="ACHK49_001231"
     CDS             2579..4408
                     /gene="recQ"
                     /locus_tag="ACHK49_001231"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO9261906.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4472..5092
                     /gene="rhtC"
                     /locus_tag="ACHK49_001232"
     CDS             4472..5092
                     /gene="rhtC"
                     /locus_tag="ACHK49_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO9261907.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5154..5774)
                     /gene="rhtB"
                     /locus_tag="ACHK49_001233"
     CDS             complement(5154..5774)
                     /gene="rhtB"
                     /locus_tag="ACHK49_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO9261908.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5885..6907
                     /gene="pldB"
                     /locus_tag="ACHK49_001234"
     CDS             5885..6907
                     /gene="pldB"
                     /locus_tag="ACHK49_001234"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO9261909.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6915..7715
                     /gene="yigL"
                     /locus_tag="ACHK49_001235"
     CDS             6915..7715
                     /gene="yigL"
                     /locus_tag="ACHK49_001235"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO9261910.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7791..8690
                     /gene="bioP"
                     /locus_tag="ACHK49_001236"
     CDS             7791..8690
                     /gene="bioP"
                     /locus_tag="ACHK49_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO9261911.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8578..9531)
                     /gene="metR"
                     /locus_tag="ACHK49_001237"
     CDS             complement(8578..9531)
                     /gene="metR"
                     /locus_tag="ACHK49_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO9261912.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9649..11910
                     /gene="metE"
                     /locus_tag="ACHK49_001238"
     CDS             9649..11910
                     /gene="metE"
                     /locus_tag="ACHK49_001238"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO9261913.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRLG"
     gene            complement(11949..12764)
                     /gene="ysgA"
                     /locus_tag="ACHK49_001239"
     CDS             complement(11949..12764)
                     /gene="ysgA"
                     /locus_tag="ACHK49_001239"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO9261914.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13026..13787
                     /gene="udp"
                     /locus_tag="ACHK49_001240"
     CDS             13026..13787
                     /gene="udp"
                     /locus_tag="ACHK49_001240"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO9261915.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            13928..15355
                     /gene="rmuC"
                     /locus_tag="ACHK49_001241"
     CDS             13928..15355
                     /gene="rmuC"
                     /locus_tag="ACHK49_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO9261916.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFSLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15450..16205
                     /gene="ubiE"
                     /locus_tag="ACHK49_001242"
     CDS             15450..16205
                     /gene="ubiE"
                     /locus_tag="ACHK49_001242"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO9261917.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16219..16824
                     /gene="ubiJ"
                     /locus_tag="ACHK49_001243"
     CDS             16219..16824
                     /gene="ubiJ"
                     /locus_tag="ACHK49_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO9261918.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            16821..18461
                     /gene="ubiB"
                     /locus_tag="ACHK49_001244"
     CDS             16821..18461
                     /gene="ubiB"
                     /locus_tag="ACHK49_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO9261919.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18540..18809
                     /gene="tatA"
                     /locus_tag="ACHK49_001245"
     CDS             18540..18809
                     /gene="tatA"
                     /locus_tag="ACHK49_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO9261920.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            18813..19328
                     /gene="tatB"
                     /locus_tag="ACHK49_001246"
     CDS             18813..19328
                     /gene="tatB"
                     /locus_tag="ACHK49_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO9261921.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19331..20107
                     /gene="tatC"
                     /locus_tag="ACHK49_001247"
     CDS             19331..20107
                     /gene="tatC"
                     /locus_tag="ACHK49_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO9261922.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20149..20931
                     /gene="tatD"
                     /locus_tag="ACHK49_001248"
     CDS             20149..20931
                     /gene="tatD"
                     /locus_tag="ACHK49_001248"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO9261923.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(20928..21416)
                     /gene="rfaH"
                     /locus_tag="ACHK49_001249"
     CDS             complement(20928..21416)
                     /gene="rfaH"
                     /locus_tag="ACHK49_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO9261924.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21583..23076
                     /gene="ubiD"
                     /locus_tag="ACHK49_001250"
     CDS             21583..23076
                     /gene="ubiD"
                     /locus_tag="ACHK49_001250"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO9261925.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23122..23823
                     /gene="fre"
                     /locus_tag="ACHK49_001251"
     CDS             23122..23823
                     /gene="fre"
                     /locus_tag="ACHK49_001251"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO9261926.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24015..25178)
                     /gene="fadA"
                     /locus_tag="ACHK49_001252"
     CDS             complement(24015..25178)
                     /gene="fadA"
                     /locus_tag="ACHK49_001252"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO9261927.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25188..27377)
                     /gene="fadB"
                     /locus_tag="ACHK49_001253"
     CDS             complement(25188..27377)
                     /gene="fadB"
                     /locus_tag="ACHK49_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO9261928.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALKRPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27567..28898
                     /gene="pepQ"
                     /locus_tag="ACHK49_001254"
     CDS             27567..28898
                     /gene="pepQ"
                     /locus_tag="ACHK49_001254"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO9261929.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            28898..29512
                     /gene="yigZ"
                     /locus_tag="ACHK49_001255"
     CDS             28898..29512
                     /gene="yigZ"
                     /locus_tag="ACHK49_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO9261930.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29551..31002
                     /gene="trkH"
                     /locus_tag="ACHK49_001256"
     CDS             29551..31002
                     /gene="trkH"
                     /locus_tag="ACHK49_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO9261931.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFLVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31014..31559
                     /gene="hemG"
                     /locus_tag="ACHK49_001257"
     CDS             31014..31559
                     /gene="hemG"
                     /locus_tag="ACHK49_001257"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO9261932.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7294 a         8072 c         8669 g         7540 t
ORIGIN      
        1 aattcttaac tttttctacg ctgcgtataa atcgcatcca tcacaaaaat tgccagcgcc
       61 acccaaataa aggcgaaagt caccatctta tcggcacccg gtttttcacc ataaaacgtc
      121 acagccagca ggaacatcag cgtcgggcca atgtactgga aaaagcctaa cgttgagaga
      181 cgcaagcgcg tggcagcggc ggtaaaacac aacagcggta cggtagtgac aataccggcg
      241 gcgatcagca gtaaattcag cgacatcggg ttttgcccca tatggctggt tgagctgtcg
      301 gcaatagcaa acaggtaaat tgccgccacg ggcagcagcc acatggtttc gattaacatg
      361 ccggtttgcg cttcaacggc aatcttcttg cgtaccagac cgtagaaggc aaaactaaat
      421 gccagtccca gcgcgataat aggtagcgaa ccaaaagtcc acagctggac taacacgcca
      481 catatcgcca gaatcaccgc cagccattgc atccggcgga atcgctcgcc gaggaaaatc
      541 atccccagca caatgttcac cagcgggtta ataaagtaac caaggctcgc ttccagcata
      601 tggtgattgt tcaccgccca gataaacagt agccagttgc caccaatcag cacggcagag
      661 actgccagca taaaaatttt ctgtggcgtc tgaatcagcg tttttaaata ggaccactgg
      721 cggcaaatgc tcatcagcac caccataaag aaaaacgacc agatcacgcg atgcgtcagg
      781 atttcatcgg caggcacgta gtaaatcaac ttgaagtacg ctggcgctat accccaaata
      841 aaataagcgg caagagcgag taatacgccc tgccgcgttt gttttgcatc catcgggaat
      901 actcattttt aattggtaac agcagtttac ctgcttttat gtcttcaacc taccatatag
      961 gtggcggtgg cactggcaat ataaagctgt tcttcattgt gtaattcaac gcgggcgacg
     1021 gcgactttat tgcctgcacg caacaggcta ctggtagcag taaaacgctc gcccctgcct
     1081 gggcgcagat aatcaacgcg aagatcaatg gtccccatcc gcgatagccg ctggcgtagt
     1141 tcatcttcac tgatggtttc gtggcgggtt aaggtacttc ccacgcacac cagacctgcg
     1201 gcgacatcca gcgctgacgc aataaccccg ccgtgcaaaa tgctttgcgc ccagttaccc
     1261 accatcattg gctgattttt aaaggccagc tgtgcgaact ctttttcgta acgctccagc
     1321 tccatcccta acgcgcggtt aaatggcatg tgataaacaa acatctcacc cactaatttc
     1381 agggcttgtt cagcggtcag tacggcagac atatcatcct tacacttcat tggttaatga
     1441 aatgttgatt ttatgcttct ttgttgttgg tttctacttt aggaagggat aactaacggc
     1501 tatggagtta agtatgtaaa atagcccgca gaaaaatatt caccttatca ataattcgtt
     1561 acggagaaca cgaccgatgc ggactctgca gggctggttg ttgccggtgt ttatgttgcc
     1621 tatggcagta tatgcacaag aggcaacggt gaaagaggtg catgacgcgc cagcggtgcg
     1681 tggcagtatt atcgccaata tgctgcagga gcatgacaat ccgttcacgc tctatcctta
     1741 tgacaccaac tacctcattt acacccaaac cagcgatctg aataaagaag cgattgccag
     1801 ttacgactgg gcggaaaatg cgcgtaagga tgaagtaaag tttcagttga gcctggcatt
     1861 tccgctgtgg cgtgggattt taggcccgaa ctcggtgttg ggtgcgtctt atacgcaaaa
     1921 atcctggtgg caactgtcca atagcgaaga gtcttcaccg tttcgtgaaa ccaactacga
     1981 accgcaattg ttcctcggtt ttgccaccga ttaccgtttt gcaggttgga cgctgcgcga
     2041 tgtggagatg gggtataacc acgactctaa cgggcgttcc gacccgacct cccgcagctg
     2101 gaaccgcctt tatactcgcc tgatggcaga aaacggtaac tggctggtag aagtgaagcc
     2161 gtggtatgtg gtgggtaata ctgacgataa cccggatatc accaaatata tgggttacta
     2221 ccagcttaaa atcggctatc acctcggtga tgcggtgctc agtgcgaaag gacagtacaa
     2281 ctggaacacc ggctacggcg gcgcggagtt aggcttaagt tacccgatca ccaaacatgt
     2341 gcgcctttat actcaggttt acagcggcta tggcgaatcg ctcatcgact ataacttcaa
     2401 ccagacccgt gtcggtgtgg gggttatgct aaacgatttg ttttgatgaa cggttgagtg
     2461 gttggcaaat ctggaaccca gcatccagga ttaccctctc agagactaaa agcattgcag
     2521 tttctcgcgc aggcgctgaa aatagcgcct gtttttattt caggcaatcg gggtgaatgt
     2581 ggcgcaggcg gaagtgttga atctggagtc cggagctaaa caggttttac aagaaacctt
     2641 tggctaccaa cagtttcgcc ccggccagga agaaattatc gacactgtgc tttccggtcg
     2701 cgattgcctg gtcgtcatgc ccaccggtgg cggaaaatcc ctttgctatc aaattcctgc
     2761 cttattgcta aacggcctta ccgtggttgt ttcaccgctg atttcgttga tgaaagatca
     2821 ggtggatcaa ctgcaagcca acggcgtggc ggcggcgtgc cttaactcga cgcaaacccg
     2881 cgaacagcaa cttgaagtga tgacaggctg ccgcaccggg caaattcgtc tgctttatat
     2941 cgccccggaa cgcctgatgc tggataactt tcttgagcat ctggcgcact ggaatccggt
     3001 gttattagcc gttgatgaag cgcactgtat ctcccaatgg ggccacgatt tccgcccgga
     3061 atatgccgcg ctcggtcagt tgcgccagcg gttcccgacg ctgccgttta tggcgctgac
     3121 cgccacagcc gacgacacca cgcgccagga tatcgtgcgc ctgctggggc tgaacgatcc
     3181 gctgattcaa atcagcagtt ttgaccgtcc gaatattcgc tacatgctga tggagaagtt
     3241 caaaccgctc gatcagttga tgcgctacgt gcaggaacag cgcggtaagt ccggcattat
     3301 ctactgcaac agccgggcga aagtagaaga caccgctgcg cgcctgcaaa gcaagggtat
     3361 tagcgcggcg gcctatcatg ccgggctgga aaataatgtc cgcgccgacg tgcaggagaa
     3421 attccagcgc gatgacctgc aaattgtggt ggcgacggtg gcgttcggca tgggcatcaa
     3481 taaaccaaac gttcgcttcg tggtccactt tgatattccg cgcaatatcg aatcctatta
     3541 tcaggaaacc ggtcgcgccg ggcgtgatgg cctgcccgcg gaagcgatgc tgttttacga
     3601 tccggccgat atggcgtggc tgcgccgttg tctggaagag aagccgcagg ggcagttgca
     3661 ggatatcgaa cgtcataaac tcaatgcgat gggcgctttt gccgaagcgc aaacttgccg
     3721 tcgtctggta ctgctgaact attttggcga agggcgtcag gagccgtgcg ggaactgcga
     3781 tatctgcctc gatccgccga aacagtacga cggttcaacc gatgctcaga ttgccctttc
     3841 caccattggt cgtgtgaatc agcgttttgg tatgggctat gtggtggaag tgattcgcgg
     3901 cgctaataac cagcgtatcc gcgactatgg tcatgacaaa ctaaaagtct acggtatggg
     3961 ccgtgataaa agtcatgaac attgggtgag cgtgatccgc cagctgattc acctcggtct
     4021 ggtgacgcaa aatattgccc agcattctgc cctacaactg acagaggctg cgcgtccggt
     4081 gctgcgcggc gaatcctctt tgcaacttgc cgtgccgcgt atcgtggcgc tcaaaccgaa
     4141 agcgatgcag aaatcgttcg gcggcaacta tgatcgcaaa ctgttcgcca aattacgcaa
     4201 actgcgtaaa tcgatagccg atgaaagtaa tgtcccgccg tacgtggtgt ttaacgacgc
     4261 aaccttgatt gagatggctg aacagatgcc gatcaccgcc agcgaaatgc tcagcgttaa
     4321 cggcgttggg atgcgcaagc tggaacgctt tggcaaaccg tttatggcgc tgattcgtgc
     4381 gcatgttgat ggcgatgacg aagagtagtc agcagcataa aaaagtgcca gtatgaagac
     4441 tccgtaaacg tttcccccgc gagtcaaatg tatgttgatg ttatttctca ccgtcgccat
     4501 ggtgcacatt gtggcgctta tgagccccgg tcccgatttc ttttttgtct ctcagaccgc
     4561 tgtcagtcgt tcccgtaaag aagcgatgat gggcgtgctg ggcattacct gcggcgtaat
     4621 ggtttgggct gggattgcgc tgcttggcct gcatttgatt atcgaaaaaa tggcctggct
     4681 gcatacgctg attatggtgg gcggtggcct gtatctctgc tggatgggtt accagatgct
     4741 acgcggtgca ctgaaaaaag aggcggtttc tgcacctgcg ccacaggtcg agctggcgaa
     4801 aagtgggcgc agtttcctga aaggtttact gaccaatctc gctaatccga aagcgattat
     4861 ctactttggc tcggtgttct cattgtttgt cggtgataac gttggcacta ccgcgcgctg
     4921 gggcattttt gcgctgatca ttgtcgaaac gctggcgtgg tttaccgtcg ttgccagcct
     4981 gtttgccctg ccgcaaatgc gccgtggtta tcaacgtctg gcgaagtgga ttgatggttt
     5041 tgccggggcg ttatttgccg gatttggcat tcatttgatt atttcgcggt gatgccagac
     5101 gcgtcttcag agtaagtcgg ataaggcgtt tacgccgcat ccgacattat ttttcacgca
     5161 tgcctcgccg atgctaacag cgctcccacc agcataaaca acgagccgaa aatcttattc
     5221 agcgccttca tctgctttgg tcctttaatc catagagcaa tccgttgagc aagggtggcg
     5281 taaccgatca tcacaataat atcgaccaca atagtggtga cgccgagcac gatatactgc
     5341 atcagttgcg gctgttgcgg catgatgaat tgcggaaata gcgccgccag aaacacaata
     5401 cttttgggat tggtgagatt cacaaaaact gcgcgctgga acaaatgtcg acgcgattga
     5461 gtagaggcca gcgatttaag gtcaattgca ccagcggcgc gccactgctg gattcccagc
     5521 caaatcaagt aagccgcgcc tgcccacttc aacacttcaa acgcaatcac tgagcgggaa
     5581 aatagcgtcc ccaaccccac gccaaccagc acaatatgaa tcgccagtcc ggtctgaagc
     5641 ccagcaatag acgccaccgc gccgcgataa ccgtggttga gcgaggtggt catagtgttg
     5701 attgcaccag agcctggcga caggcttaaa atgatcgatg tcagcaggta ggcaaaccac
     5761 cattctaagg tcatgatgaa ctcccggtgt gtctattttt gtgccacaat acgctactgt
     5821 cgcagcgttg tgtcaggcac gctaaaaaaa acgattttac gtggtttaag aggcagatta
     5881 cccgatgttt cagcagcaaa aagactggga aacaagagaa aacgcgtttg ctgcttttac
     5941 catgggaccg ctgactgatt tctggcgtca gcgtgatgaa gcagagttta ctggtgtgga
     6001 tgacattccg gtgcgctttg tccgttttcg cgcacagcac catgaccggg tggtagtcat
     6061 ctgcccgggg cgtattgaga gctacgtaaa atatgcggaa ctggcctatg acctgttcca
     6121 tttggggttt gatgtcttaa tcatcgacca tcgcgggcag ggacgttccg gtcgcctgtt
     6181 agccgatccg catctcgggc atgttaatcg ctttaatgat tatgttgatg atctggcggc
     6241 attctggcag caggaggttc agcccggtcc gtggcgtaaa cgctatatac tggcacattc
     6301 gatgggcggt gcgatctcca cattatttct gcaacgccat ccaggtgtat gtgacgccat
     6361 tgcgctaact gcgccaatgt ttgggatcgt gattcgtatg ccgtcattta tggcacggca
     6421 gatcctcaac tgggccgaag cgcatccacg tttccgtgat ggctatgcaa taggcaccgg
     6481 gcgctggcgc gcgttgccgt ttgctatcaa cgtactgacc cacagcagac agcgatatcg
     6541 acgtaactta cgcttctatg ctgatgaccc aacgattcgc gtcggtgggc cgacctacca
     6601 ttgggtacgc gaaagtattc tggctggcga acaggtgtta gccggtgcgg gtgatgacgc
     6661 cacgccaacg cttctcttac aagctgaaga ggaacgcgtg gtggataacc gcatgcatga
     6721 ccgtttttgt gaactccgca ccgccgcggg ccatcctgtc gaaggaggac ggccgttggt
     6781 aattaaaggt gcttaccatg agatcctttt tgaaaaggac gcaatgcgct cagtcgcgct
     6841 ccacgccatc gttgattttt tcaacaggca taactcaccc agcggaaacc gctctacaga
     6901 ggtttaaatt tcttatgtac caggttgttg cgtctgattt agatggcacg ttactttctc
     6961 ccgaccatac gttatcccct tacgccaaag aaactctgaa gctgctcacc gcgcgcggca
     7021 tcaactttgt gtttgcgacc ggtcgtcacc acgttgatgt ggggcaaatt cgcgataatc
     7081 tggagattaa gtcttacatg attacctcca atggtgcgcg cgttcacgat ctggatggta
     7141 atctgatttt tgctcataac ctggatcgcg acattgccag cgatctgttt ggcgtagtca
     7201 acgacaatcc ggacatcatt actaacgttt atcgcgacga cgaatggttt atgaatcgcc
     7261 atcgcccgga agagatgcgc ttttttaaag aagcggtgtt caaatatgcg ctgtatgagc
     7321 ctggattact ggagccggaa ggcgtcagca aagtgttttt cacctgcgat tcccatgaac
     7381 aactgctgcc gctggagcag gcgattaacg ctcgctgggg cgatcgcgtc aacgtcagtt
     7441 tctctacctt aacctgtctg gaagtgatgg cgggcggcgt gtcgaaaggc catgcgctgg
     7501 aagcagtggc gaagaaactg ggctacagcc tgaaggattg tattgcgttt ggtgacggga
     7561 tgaacgacgc cgaaatgctg tcgatggcgg ggaaaggctg cattatgggc agtgcgcacc
     7621 agcgtctgaa agaccttcat cccgagctgg aagtgattgg tactaatgcc gacgacgcgg
     7681 tgccgcatta tctgcgtaaa ctctatttat cgtaatcgtt ctttatttgg tcagttgtca
     7741 acctgatact tcgctacaat ggatactcgt taatcaaaga gttttccatt gtggcgctac
     7801 ttatcatcac cacgattctg tgggccttct cctttagctt ttatggcgag taccttgcgg
     7861 ggcacgtcga tagctatttt gcggtgctgg tgcgcgttgg cctggcggca ctcgtttttc
     7921 tgccgtttct gcgtacccgt ggcaatagcc tgaaaacggt cggcctgtat atgttggtgg
     7981 gcgcgatgca gctcggcgtg atgtatatgc tgagtttccg cgcgtatctc tacctgacgg
     8041 tctccgaact gctgctgttc accgtactga cgccgctcta catcacgctg atttatgaca
     8101 tcatgagtaa gcgccgtctg cgctggggct atgcctttag cgccttgttg gcggtgattg
     8161 gtgccgggat tattcgctat gatcaggtca ccgaccattt ctggactggc ttgctgctgg
     8221 tgcaactctc caatatcact tttgccatcg gcatggtggg ttacaaacgc ctgatggaaa
     8281 ctcgcccgat gccacagcat aacgcctttg cgtggttcta tcttggcgcg tttctggtgg
     8341 cggtaattgc atggttcttg ctgggaaatg cgcagaaaat gccgcaaacc acgttgcaat
     8401 ggggcattct ggtgtttctt ggcgtggtgg cttccgggat tggctacttt atgtggaact
     8461 acggcgcgac gcaggtggac gccggaacgc tgggcattat gaataatatg cacgttccgg
     8521 cagggctgct ggtaaacctg gctatctggc accaacagcc gcactggcca acgttcatta
     8581 caggcgcgct ggtgatcctg gcctcactgt gggtgcatcg taagtgggtc gctccgcgct
     8641 cttcacaaac ggcagatgat cgcaggcgtg attgcgcgct gagcgaataa atgcttctgt
     8701 aatcggctga cgctgctcgc catcgcgcac ggcggcgtac agtcggctcc acaagccttc
     8761 gcccagggtt ttggtcacca ccagaccctg gcgctcaaaa ctctctacta cccaatgcgg
     8821 tagcgcggca atacccatcc gcgcggcaac catctgaatc aacaataagg tgttatcgac
     8881 gcttttcagt gacgggctga cgcctgccgg ctgaagaaaa tgccgccaga catccagtcg
     8941 actacgctgc accggataaa tcaacagcgt ctcgctggcg agatcttccg gtgtaattcg
     9001 cgttttcgcc gccagtggat ggtcaggtgc taacaccaga cgcacttcat agtcgaacat
     9061 cggcgaataa tgcaggccac tgcgcggcag gatatcggag gtcatcacca gatccagctc
     9121 gccctgttgc aacgcgggct gcgggtcaaa cgtcacgccc gatttaaaat ccatctctac
     9181 ctgcggccag ttcttatgga aattttctaa cgcgggcgtc agccactgaa tacagctatg
     9241 gcactcaatg gcaatgcgca gacgcgtctg ctgcggttca ttgcaggctt gcagtgcctg
     9301 gctaatttgc ggcagcacct ggtttgccag ttgcaacagg atttctccct gcggtgtaaa
     9361 gcgtagcggc tggctcttac gcacaaatag ccggaagcca aggcgttgtt ccagatcgct
     9421 aaactggtga gacagggcgg attgcgtctg atgcaacgtc gccgcagcgg ctgcgagaga
     9481 gccgcagttc cgcaacgctt gtagcgtttt caggtgtttt acttcgatca tgaaagtcct
     9541 tcacttcggc atgaataatt tgcgcttgag gaatatacag taaccgccaa ttatggatgt
     9601 gtaaacatct ggacggctaa atactaaaaa ttccataagg ggcatataat gacaattctt
     9661 aatcacaccc tcggtttccc tcgcgttggc ctgcgtcgcg agctgaaaaa agcgcaagaa
     9721 agttattggg cggggaactc cacgcgtgaa gaactgctga cagtagggcg tgaactgcgt
     9781 gctcgtcact gggatcaaca aaagcaagcg ggtatcgacc tgctgccggt gggcgatttt
     9841 gcctggtacg atcatgttct gaccaccagt ctgctgctgg gtaatgttcc gccacgtcat
     9901 cagaacaaag acggttcggt agatatcgac accctgttcc gtattggtcg tggacgtgcg
     9961 ccgactggcg aacctgcggc ggcagcggaa atgaccaaat ggtttaacac caactatcac
    10021 tacatggtgc cggaattcgt taaaggccaa cagttcaaac tgacctggac gcagctgctg
    10081 gaggaagtgg acgaggcgct ggcgctgggc cacaacgtta agcctgtgct gctggggccg
    10141 gttacttacc tgtggctggg gaaagtgaaa ggtgaacagt ttgaccgcct tagcctgctg
    10201 aacgacattc tgccggttta tcagcaagtg ctggcagaac tggcgaaacg cggcatcgag
    10261 tgggtacaga ttgatgaacc cgcgctggta ctggaactac cacaggcgtg gctggacgca
    10321 tacaaacccg cttacgacgc gctccaggga caggtgaaac tgctgctgac cacctatttt
    10381 gaaggcgtaa cgccaaatct cgacacgatt actgcgctgc ctgttcaggg tctgcatgtt
    10441 gacctcgtac atggtaaaga tgacgttgct gaactgcaca agcgcctgcc ttctgactgg
    10501 ttgctgtctg cgggtctgat caatggtcgt aacgtctggc gcgccgatct taccgagaaa
    10561 tatgcgcaaa ttaaggacat tgtcggcaaa cgtgatttgt gggtggcatc ttcctgctcg
    10621 ttgctgcaca gccccatcga cctgagcgtg gaaacgcgtc ttgatgcaga agtgaaaagc
    10681 tggtttgcct tcgccctaca aaaatgccat gaactggcac tgctgcgcga tgcgctgaac
    10741 agtggtgaca cggcagctct ggcagagtgg agcgccccga ttcaggcacg tcgtcactct
    10801 acccgcgtac ataatccggc ggtagaaaag cgtctggcgg cgatcaccgc ccaggacagc
    10861 cagcgtgcga atgtctatga agtgcgtgcc gaagcccagc gtgcgcgttt taaactgcca
    10921 gcgtggccga ccaccacgat tggttctttc ccgcaaacca cggaaattcg taccctgcgt
    10981 ctggatttca aaaagggcaa tctcgacgcc aataactacc gcacgggcat tgcggaacat
    11041 atcaggcagg ccattgttga acaggaacgt ttgggactgg atgtgctggt acatggcgag
    11101 gccgagcgta atgacatggt ggaatacttt ggcgagcacc tcgacgggtt tgtctttacg
    11161 caaaacggtt gggtacagag ctacggttcc cgctgcgtga agccaccgat tgttattggt
    11221 gacgttagtc gcccggcacc gattaccgtg gagtgggcga agtatgcgca atcgctgacc
    11281 gacaaaccgg tgaaagggat gctgacgggg ccggtgacca tactctgctg gtcgttcccg
    11341 cgtgaagatg tcagccgtga aaccatcgcc aaacagattg cgctggcgct gcgtgatgag
    11401 gtggccgatc tggaagcggc agggattggt atcatccaga ttgacgaacc ggcgctgcgc
    11461 gaaggtttac cactgcgtcg tagcgactgg gacgcctatc tccagtgggg cgtagaggcc
    11521 ttccgtatca acgccgccgt ggcgaaagat gacacacaaa tccacactca catgtgttat
    11581 tgcgagttca acgacatcat ggattccatt gcggcgctgg atgcagacgt catcaccatc
    11641 gaaacctcgc gttccgacat ggagttgctg gagtcgtttg aagagtttga ttatccgaat
    11701 gaaatcggtc caggcgtcta tgacattcac tcgccaaacg taccgagcgt ggaatggatt
    11761 gaagccctgc tgaagaaagc ggcaaaacgc attccggcag agcgcctgtg ggtcaaccca
    11821 gactgtggcc tgaaaacgcg cggctggcca gaaacccgcg cggcactggc gaacatggtg
    11881 caggcggcgc agaatttgcg tctggggtaa atccaaaccg ggtggtaata ccacccggtc
    11941 ttttctcatt acagcgactt cttcccacca tactgcttaa accattccag catacgctgc
    12001 cagccatctt tcgcagattc ggcatgatag ctcgggcgat aatcagcgtt gaatgcatgc
    12061 ccggcatccg ggtagacgat aatctctgct ttcgcattag cagcccgcag cgcctggcgc
    12121 atcgtttcaa cgctctcctg cggaatgctg ttatcctgac cgccatataa gccgagaacc
    12181 ggcgcgttaa gatcggttgc gatatcaaca ggttgtttcg gtgaattcag cgacttatcg
    12241 cccgtcagtt tgccgtacca cgccactgcg gcttttagct gtggattatg cgcggcatac
    12301 agccaggtga tacgtccacc ccagcagaat ccggtgatca ttaaacgatg aacatcgccg
    12361 ccgttgcggg aagcccaact ggcgacatga tcgagatcgg ccagcacctg cgagtcaggc
    12421 acttttgcta ccagaccgct aagcaacgtg gggatatcgg caaaatcatt cggatcgcct
    12481 tcgcggaagt aaagttcagg tgcgatagcc agatacccct ccagcgccag acggcgacaa
    12541 atgtcgcgga tatgttcatg cacgccaaaa atttcctgca ctacaatgac cactggcagt
    12601 gggccatcgc tttgctttgg tctggcatgg taagcaggca tgttatcccc ttgtgaaggg
    12661 atagaggtga agcccgccac aatcgcgtcg tccggggtct gaacgatggt cgaagcgaga
    12721 ggcgatgcag caggtgcaaa tccagattgt tgtgttgttg ccatggtatt ctccgtaccc
    12781 ttataaaaat gttgcgcaat gttaactata gtcagcatgc aacaaatcac attgcctgaa
    12841 tcggctcatc ttttatgcag tcctgcagaa tgaagagtga tttatgtgat ttgcatcact
    12901 tttggtgggt aaatttatgc aacgcatttg cgtcatggtg atgagtatca cgaaaaaatg
    12961 ttaaaccctt cggtaaagtg tctttttgct tcttctgact aaaccgattc acagaggagt
    13021 tgtatatgtc caagtctgat gtttttcatc tcggcctcac taaaaacgat ttacaagggg
    13081 ctacgcttgc catcgtccct ggcgacccgg atcgtgtgga aaagatcgcc gcgctgatgg
    13141 ataagccggt taagctggca tctcaccgcg aattcaccac ctggcgtgca gagctggatg
    13201 gtaaacctgt tatcgtctgc tctaccggta tcggcggccc gtctacctct attgctgttg
    13261 aagagctggc acagctgggc attcgcacct tcctgcgtat cggtacaacg ggcgctattc
    13321 agccgcatat taatgtgggt gatgtcctgg ttaccacggc gtctgtccgt ctggatggcg
    13381 cgagcctgca cttcgcaccg ctggaattcc cggctgtcgc tgatttcgaa tgtacgactg
    13441 cgctggttga agctgcgaaa tccattggcg cgacaactca cgttggcgtg acagcttctt
    13501 ctgatacctt ctacccaggt caggaacgtt acgatactta ctctggtcgc gtagttcgtc
    13561 actttaaagg ttctatggaa gagtggcagg cgatgggcgt aatgaactat gaaatggaat
    13621 ctgcaaccct gctgaccatg tgtgcaagtc agggcctgcg tgccggtatg gtagcgggtg
    13681 ttatcgttaa ccgcacccag caagagatcc cgaatgctga gacgatgaaa caaaccgaaa
    13741 gccatgcggt gaaaatcgtg gtggaagcgg cgcgtcgtct gctgtaattc tcttctcctg
    13801 tctgaaggcc gacgcgttcg gccttttgta tttttgcgta gcgcctcgca ggaaatgcct
    13861 ttccaactgg acgtttgtac agcacaattc tattttgtgc gggtaagttg ttgcgtcagg
    13921 aggcgttgtg gatttctcaa tcatggttta cgcagttatt gcgttggtgg gtgtggcaat
    13981 tggctggctg tttgccagtt atcaacatgc gcagcaaaaa gccgagcaat tagctgaacg
    14041 tgaagagatg gtcgcggagt taagcgcggc aaaacaacaa attacccaaa gcgagcactg
    14101 gcgtgcagag tgcgagttac tcaataacga agtgcgcagc ctgcaaagta ttaacacctc
    14161 tctggaggcc gatctgcgtg aagtaaccac gcggatggaa gccgcacagc aacatgctga
    14221 cgataaaatt cgccagatga ttaacagcga gcagcgcctc agtgagcagt ttgaaaacct
    14281 cgccaaccgt atttttgagc acagcaatcg ccgggttgat gagcaaaacc gtcagagtct
    14341 gaacagcctg ttgtcgccgc tacgtgaaca actggacggt ttccgccgtc aggttcagga
    14401 cagcttcggt aaagaagcac aagaacgcca taccctgacc cacgaaattc gcaatctcca
    14461 gcaactcaac gcgcaaatgg cccaggaagc gatcaacctg acgcgcgcgc tgaaaggcga
    14521 caataaaacc cagggcaact ggggcgaggt agtattgacg cgggtgctgg aggcttccgg
    14581 tctgcgtgaa gggtatgaat atgaaaccca ggtcagcatc gaaaatgacg cccgctcgcg
    14641 gatgcagccg gatgtcatcg tgcggctgcc gcagggaaaa gatgtggtga tcgacgccaa
    14701 aatgacgctg gtcgcctatg aacgctattt taacgccgaa gacgactaca cccgcgaaag
    14761 cgcgctacag gaacatatcg cgtcggtgcg taaccatatc cgtttgctgg gacgcaaaga
    14821 ttatcaacag ctgccggggc tgcgaactct ggattacgtg ctgatgttta ttcccgttga
    14881 acccgctttt tcactggcgc ttgaccgcca gccggagctg atcaccgaag cgttgaaaaa
    14941 caatatcatg ctggttagcc cgactacgct gctggtggcg ttgcgtacta ttgccaacct
    15001 gtggcgttat gagcatcaaa gccgcaacgc ccagcaaatc gccgatcgtg ccagcaagct
    15061 gtacgacaag atgcgtttgt tcatcgatga catgtccgcg attggtcaaa gtctcgacaa
    15121 agcgcaggat aactaccgcc aggcaatgaa aaaactctct tcaggtcgcg gaaatgtgct
    15181 ggcgcaggca gaagcgtttc gcggtttagg agtagaaatt aaacgcgaga ttaatccgga
    15241 tttggctgaa caggcggtga gccaggatga agagtatcga cttcggtcgg ttccggagca
    15301 gccgaatgat gaagcttatc aacgcgatga tgaatataat cagcagtcgc gctagcccat
    15361 taggggtagt taagccgggt agaaatctag ggcatcgacg cccaatctgt tacacttctg
    15421 gaacaatttt ttgatgagca ggcattgaga tggtggataa gtcacaagaa acgacgcact
    15481 ttggttttca gaccgtcgcg aaggaacaaa aagcggatat ggtcgcccac gttttccatt
    15541 ccgtggcatc aaaatacgat gtcatgaatg atttgatgtc atttggtatt catcgtttgt
    15601 ggaagcgatt cacgattgat tgcagcggcg tacgccgtgg gcagaccgtg ctggatctgg
    15661 ctggtggcac cggcgacctg acagcgaaat tctcccgcct ggtcggagaa actggcaaag
    15721 tggtccttgc tgatatcaat gaatccatgc tcaaaatggg acgcgagaag ctgcgtaata
    15781 tcggtgtgat tggcaacgtt gagtatgttc aggcgaacgc tgaggcgctg ccgttcccgg
    15841 ataacacctt tgattgcatc accatttcgt ttggtctgcg taacgtcacc gacaaagata
    15901 aagcactgcg ttcaatgtat cgcgtgctga aacccggcgg ccgcctgctg gtgcttgagt
    15961 tctcgaagcc aattatcgag ccgctgagca aagcctatga tgcatactcc ttccatgtgc
    16021 tgccgcgtat tggctcactg gtcgcgaacg acgccgacag ctaccgttat ctggcagaat
    16081 ccatccgtat gcatcccgat caggataccc tgaaagccat gatgcaggat gccggattcg
    16141 aaagtgtcga ctactacaat ctgacggcgg gggttgtggc gctgcatcgt ggttataagt
    16201 tctgacagga gaccggaaat gccttttaaa cctttagtga cggcaggaat tgaaagtctg
    16261 ctcaacacct tcctgtatcg ctcacccgcg ctgaaaacag cccgctcgcg tctgctgggt
    16321 aaagtattgc gcgtggaggt aaaaggcttt tcgacgtcat tgattctggt gttcagcgaa
    16381 cgccaggttg atgtactggg cgaatgggca ggcgatgctg actgcaccgt tatcgcctac
    16441 gccagtgtgt tgccgaaact tcgcgatcgc cagcagctta ccgcactgat tcgcagtggt
    16501 gagctggaag tgcagggcga tattcaggtg gtgcaaaact tcgttgcgct ggcagatctg
    16561 gcagagttcg accctgcgga actgctggcc ccttataccg gtgatatcgc cgctgaagga
    16621 atcagcaaag ccatgcgcgg aggcgcaaag ttcctgcatc acggcattaa gcgccagcaa
    16681 cgttatgtgg cggaagccat tactgaagag tggcgtatgg cacccggtcc gcttgaagtg
    16741 gcctggtttg cggaagagac ggctgccgtc gagcgtgctg ttgatgccct gaccaaacgg
    16801 ctggaaaaac tggaggctaa atgacgccag gtgaagtacg gcgcctatat ttcatcattc
    16861 gcactttttt aagctacgga cttgatgaac tgatccccaa aatgcgtatc accctgccgc
    16921 tacggctatg gcgatactca ttattctgga tgccaaatcg gcataaagac aaacttttag
    16981 gtgagcgact acgactggcc ctgcaagaac tggggccggt ttggatcaag ttcgggcaaa
    17041 tgttatcaac ccgccgcgat ctttttccac cgcatattgc cgatcagctg gcgttattgc
    17101 aggacaaagt tgctccgttt gatggcaagc tggcgaagca gcagattgaa gctgcaatgg
    17161 gcggcttgcc ggtagaagcg tggtttgacg attttgaaat caagccgctg gcttctgctt
    17221 ctatcgccca ggttcatacc gcgcgattga aatcgaatgg taaagaggtg gtgattaaag
    17281 tcatccgccc ggatattttg ccggtcatta aagcggatct gaaacttatc taccgtctgg
    17341 ctcgctgggt gccgcgtttg ctgccggatg gtcgccgtct gcgcccaacc gaagtggtgc
    17401 gcgagtacga aaagaccttg attgatgaac tgaatttgct gcgggaatct gccaacgcca
    17461 ttcagcttcg gcgcaatttt gaagacagcc cgatgctcta catcccggaa gtttaccctg
    17521 actattgtag tgaagggatg atggtgatgg agcgcattta cggcattccg gtgtctgatg
    17581 ttgcggcgct ggagaaaaac ggcactaaca tgaaattgct ggcggaacgc ggcgtgcagg
    17641 tgttcttcac tcaggtcttt cgcgacagct ttttccatgc cgatatgcac cctggcaaca
    17701 tcttcgtaag ctatgaacac ccggaaaacc cgaaatatat cggcattgat tgcgggattg
    17761 ttggctcgct aaacaaagaa gataaacgct atctggcaga aaactttatc gccttcttta
    17821 atcgcgacta tcgcaaagtg gcagagctac acgtcgattc aggctgggtg ccaccagata
    17881 ccaacgttga agagttcgaa tttgccattc gtacggtctg tgaacctatc tttgagaaac
    17941 cgctggccga aatttcgttt ggacatgtac tgttaaatct gtttaatacg gcgcgtcgct
    18001 tcaatatgga agtgcagccg caactggtgt tactccagaa aaccctgctc tatgtcgaag
    18061 gggtaggacg ccagctttat ccgcagctcg atttatggaa aacagcgaag cctttcctgg
    18121 agtcgtggat taaagatcag gtcggtattc ctgcgctggt gagagcattt aaagaaaaag
    18181 cgccgttctg ggtcgaaaaa atgccagaac tgcctgaatt ggtttacgac agtttgcgcc
    18241 agggcaagta tttacagcac agtgttgata agattgcccg cgagcttcag tcaaatcatg
    18301 tacgtcaggg acaatcgcgt tattttctcg gaattggcgc tacgttagta ttaagtggca
    18361 cattcttgtt ggtcagccga cctgagtggg ggctgatgcc cggctggtta atggcaggtg
    18421 gtctgatcgc ctggtttgtc ggttggcgca aaacacgctg attttttcat cgctcaaggc
    18481 gggccgtgta acgtataatg cggctttgtt taatcatcat ctaccacaga ggaacatgta
    18541 tgggtggtat cagtatttgg cagttattga ttattgccgt catcgttgta ctgctttttg
    18601 gcaccaaaaa gctcggctcc atcggttccg atcttggtgc gtcgatcaaa ggctttaaaa
    18661 aagcaatgag cgatgatgaa ccaaagcagg ataaaaccag tcaggatgct gattttactg
    18721 cgaaaactat cgccgataag caggcggata cgaatcagga acaggctaaa acagaagacg
    18781 cgaagcgcca cgataaagag caggtgtaat ccgtgtttga tatcggtttt agcgaactgc
    18841 tattggtgtt catcatcggc ctcgtcgttc tggggccgca acgactgcct gtggcggtaa
    18901 aaacggtagc gggctggatt cgcgcgttgc gttcactggc gacaacggtg cagaacgaac
    18961 tgacccagga gttaaaactc caggagtttc aggacagtct gaaaaaggtt gaaaaggcga
    19021 gcctcactaa cctgacgccc gaactgaaag cgtcgatgga tgaactacgc caggccgcgg
    19081 agtcgatgaa gcgttcctac gttgcaaacg atcctgaaaa ggcgagcgat gaagcgcaca
    19141 ccatccataa cccggtggtg aaagataatg aagctgcgca tgagggcgta acgcctgccg
    19201 ctgcacaaac gcaggccagt tcgccggaac agaagccaga aaccacgcca gagccggtgg
    19261 taaaacctgc tgcggacgct gaaccgaaaa ccgctgcacc ttccccttcg tcgagtgata
    19321 aaccgtaaac atgtctgtag aagatactca accgcttatc acgcatctga ttgagctgcg
    19381 taagcgtctg ctgaactgca ttatcgcggt gatcgtgata ttcctgtgtc tggtctattt
    19441 cgccaatgat atctatcacc tggtatccgc gccattgatc aagcagttgc cgcaaggttc
    19501 aacgatgatc gccaccgacg tggcctcgcc gttctttacg ccgatcaagc tgacctttat
    19561 ggtgtcgctg attctgtcag cgccggtgat tctctatcag gtgtgggcat ttatcgcccc
    19621 agcgctgtat aagcatgaac gtcgcctggt ggtgccgctg ctggtttcca gctctctgct
    19681 gttttatatc ggtatggcat tcgcctactt tgtggtcttt ccgctggcat ttggcttcct
    19741 tgccaatacc gcgccggaag gggtgcaggt atccaccgac atcgccagct atttaagctt
    19801 cgttatggcg ctgtttatgg cgtttggtgt ctcctttgaa gtgccggtgg caattgtgct
    19861 gctgtgctgg atggggatta cctcgccaga agacttacgc aaaaaacgcc cgtatgtgct
    19921 ggttggtgca ttcgttgtcg ggatgttgct gacgccgccg gatgtcttct cgcaaacgct
    19981 attggcgatc ccgatgtact gtctgtttga aatcggtgtc ttcttctcac gcttttacgt
    20041 tggtaaaggg cgaaatcggg aagaggaaaa cgacgctgaa gcagaaagcg aaaaaactga
    20101 agaataaatt caaccgcccg tcagggcggt tgtcatatgg agtacaggat gtttgatatc
    20161 ggcgttaatt tgaccagttc gcaatttgcg aaagaccgtg atgatgttgt agcgcgcgct
    20221 tttgacgcgg gagttaatgg gctactcatc accggcacta acctgcgtga aagccagcag
    20281 gcgcaaaagc tggcgcgtca gtattcgtcc tgttggtcaa cggcgggcgt acatcctcac
    20341 gacagcagcc agtggcaagc tgcgactgaa gaagcgatta ttgagctggc cgcgcagcca
    20401 gaagtggtgg cgattggtga atgtggtctc gactttaacc gcaacttttc gacgccggaa
    20461 gagcaggaac gcgcttttgt tgctcagcta cgcattgccg cagaattaaa catgccggta
    20521 tttatgcact gtcgcgatgc ccacgagcgg tttatgacat tgctggagcc gtggctggat
    20581 aaactgcctg gtgcggttct tcattgcttt accggcacac gcgaagagat gcaggcgtgc
    20641 gtggcgcgtg gaatttatat cggcattacc ggttgggttt gcgatgaacg acgcgggctg
    20701 gagctgcggg aattgttgcc gttgattccg gcggagaaat tgctgatcga aactgatgcg
    20761 ccgtatctgc tcccgcgcga tctcacgcca aagccatcat cccggcgcaa cgagccagcc
    20821 catctgcccc atattttgca acgtattgcg cactggcgtg gagaagatgc cgcatggctg
    20881 gctgccacca cggatgctaa tgtcaaaaca ctgtttggga ttgcgtttta gagtttgcgg
    20941 aactcggtgt tcttcacact gtgcttaatc tctttattaa taagattaag caatagcatg
    21001 gagcgagcct caccatcggg ttcggtgaaa atggcctgaa agccttcgaa cgcgccttcg
    21061 gtaataatca ccttatcacc cggataaggg gttgccggat cgacaatgtc tttcggttta
    21121 tataccgata gctgatgaat caccgccgat gggactatcg ctggcgacgc gccaaagcgc
    21181 acgaagtggc tgacaccgcg ggtcgcgttg atagtcgtgg tatgaatcac ttctggatca
    21241 aattcgacaa acaggtagtt gggaaacaat ggctcactga ctgcagtacg ttttccacgc
    21301 acgatttttt ccagggtgat catcggtgcc aggcaattca cagcctgtct ttcgaggtgt
    21361 tcctgggcac gttgaagttg cccgcgcttg cagtacagta aataccagga ttgcataatg
    21421 actcttatcc gtttaatcgg ggcgcaagga tagcaaaagc tttacgctaa gttaattata
    21481 ttccccggtt tgcgttatac cgtcagagtt cacgctaatt taacaaattt acagcatcgc
    21541 aaagatgaac gccgtataat gggcgtagat taagaggcta caatggacgc catgaaatat
    21601 aacgatttac gcgacttctt gacgctgctt gaacagcagg gtgagctaaa acgtatcacg
    21661 ctcccggtgg atccgcatct ggaaatcact gaaattgctg accgcacttt gcgtgccggt
    21721 gggcctgcgc tgttgttcga aaaccctaaa ggctactcaa tgccggtgct gtgcaacctg
    21781 ttcggtacgc caaagcgcgt ggcgatgggc atggggcagg aagatgtttc ggcgctgcgt
    21841 gaagttggta aattattggc gtttctgaaa gagccggagc cgccaaaagg tttccgcgac
    21901 ctgtttgata aactgccgca gtttaagcaa gtattgaaca tgccgacaaa gcggctgcgt
    21961 ggtgcgccct gccaacaaaa aatcgtctct ggcgatgacg tcgatctcaa tcgcattccc
    22021 attatgacct gctggccgga agatgccgcg ccgctgatta cctgggggct gacagtgacg
    22081 cgcggcccgc ataaagagcg gcagaatctg ggcatttatc gccagcagct gattggtaaa
    22141 aacaaactga ttatgcgctg gctgtcgcat cgcggcggcg cgctggatta tcaggagtgg
    22201 tgtgcggcgc atccgggcga acgtttcccg gtttctgtgg cgctgggtgc cgatcccgcc
    22261 acgattctcg gtgcagtcac ccccgttccg gatacacttt cagagtatgc gtttgccgga
    22321 ttgctgcgcg gtaccaagac cgaagtggtg aagtgtatct ccaatgacct tgaagtgccc
    22381 gccagtgcgg agattgtgct ggaagggtat atcgaacaag gtgaaacggc gccggaaggg
    22441 ccgtatggcg accacaccgg ttactataac gaagtcgata gtttcccggt atttaccgtg
    22501 acgcatatta cccagcgtga agatgcgatt taccattcca cctataccgg gcgtccgcca
    22561 gatgagcccg cggtactggg cgtggcgttg aacgaagtgt ttgtaccgat tctgcaaaaa
    22621 cagttcccgg aaattgtcga tttttacctg ccgccggaag gctgttctta tcgtctggcg
    22681 gtagtgacga tcaaaaaaca gtacgccgga cacgcgaagc gcgtcatgat gggcgtctgg
    22741 tcgttcttac gccagtttat gtacactaaa tttgtgatcg tttgcgatga tgacgtcaac
    22801 gcacgcgact ggaacgatgt gatttgggcg attaccaccc gtatggaccc ggcgcgggat
    22861 actgttctgg tagaaaatac gcctattgat tatctggatt ttgcctcgcc tgtctccggg
    22921 ctgggttcaa aaatggggct ggatgccacg aataaatggc cgggggaaac ccagcgtgaa
    22981 tggggacgtc ccatcaaaaa agatccagat gttgtcgcgc atattgacgc catctgggat
    23041 gaactggcta tttttaacaa cggtaaaagc gcctgatgcg cgtttgtttt gccctattta
    23101 tcgatccgac agagaaagcg catgacaacc ttaagctgta aagtgacctc ggtagaagct
    23161 atcacggata ccgtatatcg tgtccgcatc gtgccagacg cggccttttc ttttcgtgct
    23221 ggtcagtatt tgatggtagt gatggatgag cgcgacaaac gtccgttctc aatggcctcg
    23281 acgccggatg aaaaagggtt tatcgagctg catattggcg cttctgaaat caacctttac
    23341 gcgaaagcag tcatggaccg catcctcaaa gatcatcaaa tcgtggtcga tattccccac
    23401 ggagaagcgt ggctgcgcga tgatgaagag cgtccgatga ttttgattgc gggcggcacc
    23461 gggttctctt atgcccgctc gattttgctg acagcgttgg cgcgtaaccc aaaccgtgat
    23521 atcaccattt actggggcgg gcgtgaagag cagcatctgt atgatctctg cgagcttgag
    23581 gcgctttcgt taaagcaccc tggtctgcaa gtggtgccgg tggttgaaca accggaagca
    23641 ggctggcgtg ggcgtactgg taccgtgtta acggcggtat tgcaggatca tggtacgctg
    23701 gcagagcatg atatctatat tgccggacgt tttgagatgg cgaaaattgc ccgtgacctg
    23761 ttttgcagtg agcgtaatgc gcgggaagat cgcctgtttg gcgatgcgtt tgcatttatc
    23821 tgagatataa aaaaacccgc ccctgacagg cgggaagaac ggcaactaaa actttattca
    23881 gtggcattta gatctatgtc gcatccggca atcggtgcac gatgcctgat gcgacgctgg
    23941 cgcgtcttat caggcctaca ttggtgcagg atcggtagac cggataaggc gttcacgccg
    24001 catccggcaa gtggttaaac ccgctcaaac accgtcgcaa taccctgacc cagaccgata
    24061 cacatcgtcg ccagaccaaa ctgaacgtct ttgcgttcca tcagattcag cagcgtggtg
    24121 ctgatacgcg caccggaaca gcccagcgga tgacccagcg cgatcgcacc accgttgagg
    24181 ttgatcttct cgtcaatctg ctcaatcagt cccagatctt taatacatgg caggatctgc
    24241 gcggcaaagg cttcgttcat ctcaaacacg ccgatatcgc tggcagaaag ccccgctttt
    24301 ttcagcgcca gcttcgaggc cggaaccggg ccgtaaccca taatcgatgg gtcacaacca
    24361 acgaccgcca tcgaacgcac acgagcgcgc ggcttaagac ctaattcatg ggcgcggctt
    24421 tcactcatca ccagcatggc agctgcgcca tcggaaagtg cagaagatgt gcccgccgtt
    24481 accgtaccgt ttactggatc aaacgccgga cgcagcgtgg cgagggcttc cacggtggtt
    24541 tccgggcgaa tcacttcgtc gtaattaaac tgcttcagga cgccgtcggc atcgtgacca
    24601 ccagttggga tgatttcatt tttaaatgcg gccgactgcg tggcggccca ggcgcgggcg
    24661 tgtgaccgcg cggcaaaggc atcctgcatt tcacggctga taccgtgcat acgcgccagc
    24721 atttctgctg ttaagcccat catgcccgcc gctttggcga cattgcggct caggccgggg
    24781 tgaaaatcga cgccgtgact catcggcaca tggcccatat gctccacgcc gccaaccaga
    24841 catgcctgcg catcgccggt cataatcatc cgtgctgcgt catgcagtgc ctgcatggat
    24901 gaaccacaca agcgattgac ggtaaccgcc gggacagagt gtggtacttc tgccagcagc
    24961 gccgcgttac gggcgatgtt aaaaccctgc tccagcgtct gctgcacaca accccagtaa
    25021 atatcgtcga gggccgccgc ttccagcgcc gggttacgcg ccagcaggct acgcattaaa
    25081 tgagcggaga gatcttctgc acgcacgtta cgaaaagcac cgcccttcga acggcccatc
    25141 ggggtgcgaa ttgcatcgac aatgacaacc tgttccattg tgactcctta agccgttttc
    25201 aggtcgccaa ccggacgggc tggctcaacc ggaggatagt acggttcgtt atgacgcgct
    25261 ttattacgca gaccttccgg cacttcatac agcgggccga ggtgctgata ttgctgtgcc
    25321 atatcgaggt attttgcgct accgagggtg tccagccagc ggaacgcgcc gccgtggaac
    25381 ggagggaagc ccaggccgta gaccagcgcc atatccgctt ccgccggagt ggcgataatg
    25441 ccttcctcca gacagcgcac cacttcgttg accatcggga tcatcatgcg ggcgataatc
    25501 tcctcttcgc tgaaatcgcg cttcggctgg ctcacttctg ccagcaggtc ttcaacggcg
    25561 gcgtcttctt ctttcttcgg cttacctttg ctgtcttctt tataacgcca gaaaccgagg
    25621 ccgttcttct gaccaaagcg gttggcatca aacagcgcgt cgatggcatc gcggtaatct
    25681 ttctgcatcc tctgcgggaa gcctgctgcc atgacagcct gggcatgatg cgcggtgtca
    25741 atgcccacga catccagcag atatgccgga cccatcggcc agccaaactg tttttccatc
    25801 actttgtcga tcttgcggaa atccgcgccg tcgcgcagca gctggctgaa accggcgaaa
    25861 tacgggaaca gcacgcggtt aacaaagaag ccggggcagt cgttaaccac aatcggcgtc
    25921 ttgcccatct tgctcgccca ggcgacaact ttcgcgatgg tttcgtcgga gcttttctcg
    25981 ccgcgaataa tttctaccaa cggcattcgg tggaccgggt taaagaagtg catcccgcag
    26041 aagttttccg ggcgtttcag cgcgttggcc agttcgctga taggaatggt tgaagtgtta
    26101 gacgccagca cggtatcctg gcgtactttt tgttcggttt ctgccagtac ggcttttttc
    26161 actttcgggt tttcaacaac cgcttctacc acaatatcca cgcggtcaaa tccggcgtag
    26221 tcgagcgttg ggtggattgt ggagatcacg ccagccagtt tcagaccatc gatcttgccg
    26281 cgctcaagct gcttgttcag cagtttcgcg gcttcggtca tgccgagggt taacgacttg
    26341 tcgttgatat ctttcatgac aaccggcacg cctttccacg cagactggta agcgatgccg
    26401 ccgcccataa tgcctgcacc cagcaccgcg gcctgtttcg gggtttcaac gtctttggtg
    26461 agtttcttcg ctttgccttt tacatattga tcgttaagga aaatgccgac cagtgcgcgg
    26521 gcttcgttgg tatgcgccag cgggacaaaa cttttgtttt ccaggtttaa ggcttcttca
    26581 cgaccaaaac gggccgcagc ttcaatggtt tttactgcgg tgatgggggc cggataatgt
    26641 ttccccgctg tttgtgcgac catcccttta gcgatggtga agctcatggt ggcttcaatc
    26701 ttgctcagtt ttagtggttc cagcttcggc tgacgttttg ctttccagtc gaggtcgccg
    26761 ttaatggcct ggcgtaaaac cgcctttgcg ccttcaacca gtttttctgc tttgactacg
    26821 ccatccacca gaccgatttt cagcgcctga tccgcgccga catctttacc ggcggcaatg
    26881 atttccagcg cactgtctgc gcccagcata cgtggcatac gtacagaacc gccaaagccc
    26941 ggcatgatgc ccagtttggt ttccggcaga ccgatgcgca gatccggcgt cgccagacga
    27001 taatcggtcg ccagcacgca ttcgcagcca ccgcccagcg catagccgtt gacggcagca
    27061 atggtcggca ccggcagatc ttccaggcga ttaaacacgc tattggcaaa gtgcagccac
    27121 tgacttaact gttcttcagg aacgaggaac agggacaaaa attcggtgat atcagcaccg
    27181 acgataaagg ctgctttgtt cgaacgcagc agcagccctt ttagatctga ttgctgttcc
    27241 agcacgccga tggcctcgcc gaggctggcg acggtcgcag tgtcgagttt attaactgaa
    27301 cctggggcat caaataccag ttcggcaatg ccatcttcca gccagtcaag gtacagggtg
    27361 tcgcctttgt aaagcatgtc agtctcctga atccgcaagg tgatctggtc gtaccagatg
    27421 catcgaagtg tgtattttgt gttaaaaata tgcaaataaa agattaaaga aatgccgatc
    27481 tgatcacgct cggcagaaat cacgctctgg atgaacgatg tgctaagatg cggagactta
    27541 aggtcaaaaa aacagaaggg taaaaaatgg aatcactggc ctcactctat aaaaatcata
    27601 tagctacctt acaggaacgg actcgcgatg cgctggcgcg cttcaagctg gatgcgttac
    27661 ttattcactc cggcgaactg ttcaacgttt ttctcgacga tcatccctat ccgtttaaag
    27721 tgaacccgca attcaaagcg tgggtgccgg taactcaggt gccaaactgc tggctgctgg
    27781 tggatggcgt gaacaagccg aaactgtggt tctatctgcc ggttgattac tggcacaacg
    27841 tcgaaccgct gccgacctct ttctggactg aagacgtgga agtgatcgcg ctgccgaaag
    27901 ccgatggcat tggtagcctg ctgcctgctg cgcgcgggaa tatcggttat atcggtccgg
    27961 tgccggaacg tgcgctgcaa ctgggtattg aggccagcaa tatcaacccg aaaggggtga
    28021 tcgactacct gcattactac cgctccttca aaaccgagta cgaactggcc tgtatgcgtg
    28081 aagcgcagaa aatggcggtc aacggtcatc gcgcggcaga agaagcgttc cgttctggca
    28141 tgagcgagtt cgatatcaac attgcctatc tgaccgcgac cggtcatcgt gataccgacg
    28201 taccttacag caacattgtg gcgctcaacg aacacgctgc ggtgttgcat tacaccaaac
    28261 tggaccacca ggcaccggaa gagatgcgca gcttcctgct ggatgccggg gccgaatata
    28321 acggctatgc cgctgacctg acccgtacct ggtcggcaaa aagtgacaac gattacgcac
    28381 agctggtgaa agacgtaaat gatgaacaac tggcgctgat cgcgaccatg aaagctggcg
    28441 ttagctatgt ggattaccac atccagttcc atcagcgcat cgccaaactg ctgcgtaaac
    28501 atcaaatcat caccgatatg agtgaagaag cgatggtcga aaacgatctc accggaccgt
    28561 ttatgccgca cggtatcggc catccgctgg gcctgcaggt gcatgacgtc gccggtttta
    28621 tgcaggatga tagcggtacg cacctcgcgg caccggcaaa atatccgtac ctgcgctgca
    28681 cccgtattct ccagccgggc atggtgttaa ccatcgaacc gggtatctac ttcattgaat
    28741 cgctactggc accgtggcgt gaagggcagt tcagcaagca cttcaactgg cagaaaattg
    28801 aagcactgaa accgttcggc ggcattcgta tcgaagacaa cgtggtgatc cacgaaaata
    28861 acgtggaaaa catgacccgg gatctgaaac tggcgtgatg gaaagctggt taattcctgc
    28921 ggcaccggtc acggtcgttg aagagatcaa aaagagccgc ttcataacga tgttggcgca
    28981 taccgatggc gttgaggcgg cgaaagcgtt tgttgaatcg gtgcgggcag aacaccccga
    29041 tgcccgccac cattgcgtgg cgtgggtggc gggtgcgccg gatgattcac aacagctggg
    29101 tttctctgac gacggggagc cggcgggaac ggcaggtaaa ccgatgctcg cccagctaat
    29161 gggcagcggc gttggggaaa ttaccgctgt ggtggtgcgc tactacggcg gcatattgct
    29221 tggcaccggt gggttagtga aagcgtatgg cggcggcgtg aatcaggcgc tgcgccagct
    29281 aacgacccaa cgcaagacgc cattaaccga atatactttg caatgtgaat atcatcagtt
    29341 aaccggcatt gaagcgttgc tggggcagtg tgacggcaaa attatcaaca gtgattatca
    29401 ggcattcgtt ctgctgcggg tggcgcttcc ggcggcgaaa gtggctgaat tttccgcaaa
    29461 gctggcggat tttagccgtg gttcattgca attgttagcg attgaagaat aatccccact
    29521 tcgttttgca gaactaagga agcggcagag atgcattttc gcgccattac ccgaatcgtt
    29581 ggactactgg tcatcttatt ttcagggacc atgattatcc ctgggctggt agcactcatc
    29641 taccgggatg gagcgggccg cgcttttacc cagacctttt ttgtcgccct cgccattggc
    29701 tctatgctgt ggtggccgaa ccgcaaagag aaaggcgaac ttaaatcccg tgaggggttt
    29761 ctgatagtgg tgctgttctg gaccgtgctg ggtagcgtcg gtgcgctccc ttttatcttc
    29821 tcggaaagcc cgaacctcac gattaccgat gcgttttttg aatctttctc tggcctgacc
    29881 accaccgggg ccactacgct ggtggggctg gattcgctcc ctcacgccat cctcttttat
    29941 cgccagatgc tgcaatggtt tggcgggatg gggatcatcg tgttagcggt tgcgatactg
    30001 cctatcctcg gcgtgggtgg gatgcagctc tatcgcgcag aaatgcccgg cccgctgaaa
    30061 gataacaaaa tgcgcccgcg aattgcggaa acggcgaaaa ccctgtggtt gatttatgtc
    30121 ttgctgaccg tcgcctgtgc gctggcgttg tggtttgccg gaatggatgc ctttgatgcc
    30181 atcggccata gctttgcgac tatcgctatt ggcggcttct cgacacatga tgccagtatc
    30241 ggttatttcg atagcccgac tattaacact atcattgcta tcttcctgct gatctccggc
    30301 tgtaactacg gtctgcactt ttcactgtta agtgggcgta gtctgaaggt ttattggcgc
    30361 gatccggaat ttcgcatgtt tatcggcgta cagtttacgc tggtggttat ttgtaccctc
    30421 gtactgtggt ttcataatgt ctacagttcg gcgctgatga caattaacca ggcgtttttc
    30481 ctggtggtgt cgatggcgac aaccgccggg tttacgactg acagcattgc ccgctggccg
    30541 ctctttttgc cggtactgct tttatgttcg gcttttatcg gcggttgtgc cgggtcaacg
    30601 ggcggtggcc tgaaagtgat ccgcatcctg ctgctgttta agcaggggaa ccgtgagctg
    30661 aaacgactgg tgcatccgaa cgccgtctat agcattaagc tggggaatcg cgcactgccg
    30721 gaacgtatcc tcgaagccgt ttggggattt ttctccgcct atgcattggt gtttattgtc
    30781 agtatgctgg cgattatcgc cacgggcgtg gatgactttt ctgcctttgc ctcagttgtt
    30841 gcgacattga ataacctggg gccagggctt ggcgtggttg ctgataactt taccagtatg
    30901 aacccggtgg ctaaatggat cctgattgcc aacatgctgt ttggtcgtct cgaggtcttt
    30961 acattgctgg tgctctttac cccgactttc tggcgtgaat gatggagtaa tacgtgaaaa
    31021 cattaattct tttctcaaca agggacggac aaacgcgcga gattgcctcc tacctggctt
    31081 cggaactgaa agaactgggg atccagacgg atgtcgccaa tgtgcaccgc attgaagaac
    31141 cacaatggga aaattatgac cgtgtggtca ttggtgcttc tattcgctat ggtcactacc
    31201 actctgcgtt ccaggaattt gtcaaaaaac atgcgacgcg actgaattcg atgccgagcg
    31261 ccttttattc cgtaaacctg gtggcgcgca aaccggagaa gcgtactcca cagaccaaca
    31321 gctacgcgcg gaagtttctg atgaactcgc aatggcgtcc cgatcgctgc gcggtcattg
    31381 ccggggcgct gcgttaccca cgttatcgct ggtacgaccg ttttatgatc aagctgatta
    31441 tgaagatgtc aggcggtgaa acggatacgc gcaaagaagt tgtctatacc gattgggagc
    31501 aggtggcgaa tttcgcccga gaaatcgccc atttaaccga caaaccgacg ctgaaataag
    31561 cacaaagaat aaaaa
//
LOCUS       DAZGLJ010000029        31075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000029 DAZGLJ010000000
VERSION     DAZGLJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.029"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            248..631
                     /gene="secE"
                     /locus_tag="ACHK49_001258"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="ACHK49_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO9261933.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="ACHK49_001259"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="ACHK49_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO9261934.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="ACHK49_001260"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="ACHK49_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO9261935.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="ACHK49_001261"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="ACHK49_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO9261936.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="ACHK49_001262"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="ACHK49_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO9261937.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="ACHK49_001263"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="ACHK49_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO9261938.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="ACHK49_001264"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="ACHK49_001264"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO9261939.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="ACHK49_001265"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="ACHK49_001265"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO9261940.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12676..13215
                     /gene="yjaZ"
                     /locus_tag="ACHK49_001266"
     CDS             12676..13215
                     /gene="yjaZ"
                     /locus_tag="ACHK49_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HFO9261941.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13625..14758)
                     /gene="thiH"
                     /locus_tag="ACHK49_001267"
     CDS             complement(13625..14758)
                     /gene="thiH"
                     /locus_tag="ACHK49_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO9261942.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14755..15525)
                     /gene="thiG"
                     /locus_tag="ACHK49_001268"
     CDS             complement(14755..15525)
                     /gene="thiG"
                     /locus_tag="ACHK49_001268"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO9261943.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15527..15727)
                     /gene="thiS"
                     /locus_tag="ACHK49_001269"
     CDS             complement(15527..15727)
                     /gene="thiS"
                     /locus_tag="ACHK49_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO9261944.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15711..16466)
                     /gene="thiF"
                     /locus_tag="ACHK49_001270"
     CDS             complement(15711..16466)
                     /gene="thiF"
                     /locus_tag="ACHK49_001270"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO9261945.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16459..17094)
                     /gene="thiE"
                     /locus_tag="ACHK49_001271"
     CDS             complement(16459..17094)
                     /gene="thiE"
                     /locus_tag="ACHK49_001271"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO9261946.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17094..18989)
                     /gene="thiC"
                     /locus_tag="ACHK49_001272"
     CDS             complement(17094..18989)
                     /gene="thiC"
                     /locus_tag="ACHK49_001272"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO9261947.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19070..19170)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19222..19698)
                     /gene="rsd"
                     /locus_tag="ACHK49_001273"
     CDS             complement(19222..19698)
                     /gene="rsd"
                     /locus_tag="ACHK49_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO9261948.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19793..20566
                     /gene="nudC"
                     /locus_tag="ACHK49_001274"
     CDS             19793..20566
                     /gene="nudC"
                     /locus_tag="ACHK49_001274"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO9261949.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20606..21670
                     /gene="hemE"
                     /locus_tag="ACHK49_001275"
     CDS             20606..21670
                     /gene="hemE"
                     /locus_tag="ACHK49_001275"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO9261950.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21680..22351
                     /gene="nfi"
                     /locus_tag="ACHK49_001276"
     CDS             21680..22351
                     /gene="nfi"
                     /locus_tag="ACHK49_001276"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO9261951.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22394..22984
                     /gene="yjaG"
                     /locus_tag="ACHK49_001277"
     CDS             22394..22984
                     /gene="yjaG"
                     /locus_tag="ACHK49_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO9261952.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGDSNIGIIFQQ"
     gene            23171..23443
                     /gene="hupA"
                     /locus_tag="ACHK49_001278"
     CDS             23171..23443
                     /gene="hupA"
                     /locus_tag="ACHK49_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO9261953.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23456..24151
                     /gene="yjaH"
                     /locus_tag="ACHK49_001279"
     CDS             23456..24151
                     /gene="yjaH"
                     /locus_tag="ACHK49_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO9261954.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRAAQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24153..24578)
                     /gene="zraP"
                     /locus_tag="ACHK49_001280"
     CDS             complement(24153..24578)
                     /gene="zraP"
                     /locus_tag="ACHK49_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO9261955.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24816..26192
                     /gene="zraS"
                     /locus_tag="ACHK49_001281"
     CDS             24816..26192
                     /gene="zraS"
                     /locus_tag="ACHK49_001281"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO9261956.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVGQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26189..27514
                     /gene="zraR"
                     /locus_tag="ACHK49_001282"
     CDS             26189..27514
                     /gene="zraR"
                     /locus_tag="ACHK49_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO9261957.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDTLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCVALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLASHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERSVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTCGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27511..28800)
                     /gene="purD"
                     /locus_tag="ACHK49_001283"
     CDS             complement(27511..28800)
                     /gene="purD"
                     /locus_tag="ACHK49_001283"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO9261958.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28812..30401)
                     /gene="purH"
                     /locus_tag="ACHK49_001284"
     CDS             complement(28812..30401)
                     /gene="purH"
                     /locus_tag="ACHK49_001284"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO9261959.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7355 a         8042 c         8486 g         7192 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcatttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat cctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaacatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg ggccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcacc gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgtt ctgggtctgt actatatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgcaaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggta tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgtgatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcgaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatatcctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aagtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaatactg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagta ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggtca gaccattacg cgtcagacag acgaattgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgattc cgggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacaccc tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagca cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgttactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgttaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atggggggag tttagggaaa gagcatttgt
    12541 cagaatattt aaggaatttc tgaatactca taatcaatgt agagatgact aatatcctga
    12601 aactgactga actaattgag tcaaactcgg caaggattcg atactattcc tgtgtaactt
    12661 tcttaaggaa cgagaatgaa acaggaagtg gaaaagtggc gaccttttgg acatccggat
    12721 ggtgatattc gtgatttatc atttcttgat gctcatcagg ctgtctacgt tcagcatcat
    12781 gagggcaaag agcctttaga gtatcgcttt tgggttacct actctcttca ctgcttcaca
    12841 aaagattatg aacatcagac gaacgaagaa aaacaatcgt taatgtacca cgcgcctaaa
    12901 gaatctcgtc ccttctgcca gcaccgttat aacttagcgc gcacacactt aaaaagaact
    12961 attttggcgc tgccagaaag caacgttatt catgccgggt atggtagcta tgccgtgatt
    13021 gaggtggact tagacggagg agataaggca ttttactttg ttgcgttcag ggctttcagg
    13081 gaaaagaaaa aactccgttt gcatgtaact agcgcttatc ccatttctga aaaacagaaa
    13141 ggtaaatcag tgaaattttt caccattgcc tacaacttat tgagaaataa gcagcttcct
    13201 cagccctcaa aataacaaaa cccaccttaa ggtgggtttc gccagagaat tatctctggt
    13261 attcagaacg ccattaccgg actttgcctt gaccttgcga taatcgcagg ttgcgggatg
    13321 tctgaatttc ttcagtctgc tgcatcctgg aagatgagaa catgtgttct tattttcgtc
    13381 tctatcatag ttgagtattt actctcttac aatcagatct ctttcattgc tcaacaggcg
    13441 atggcttcag actttgcatt acggaatttt taagaaaggc agggcgaaac gaggaagaag
    13501 cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca caacgctttc
    13561 aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt tcttacattc
    13621 cgtctcatag tctttgcgag gcccgtccca gatagctgtc ccagtctttc cataccggct
    13681 gcaaaccctg agcggttaac gcggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg
    13741 agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt ttcgagaagg
    13801 cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt gattcccgcg
    13861 tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta
    13921 actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg cgcaggcgcg
    13981 gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc aaatgttcgg
    14041 caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg ccgaggccta
    14101 tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag aagaagtcct
    14161 gtttttttcc tttcagatga tggcgggcat aagtcgcctc gtgatatgtc tcctgataaa
    14221 ccatcacgcc atccagacca agctgcttta actcggcgta ttccgtctcc gccagcggtt
    14281 gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga cgacgaaagt
    14341 aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt tcaaagccca
    14401 tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt ttgcgcttga
    14461 tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat
    14521 aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga cgctgcgccc
    14581 gttgggccag ttgttccaga tagccactgg cggctggcga taacagcgcc atcatatcgt
    14641 cgcgggtgag ttgcgaggca tttagcgccc gttctacgtc agcagccgtt ttgccgttga
    14701 tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg atcgctgaag gttttcatgc
    14761 cgatgcctcc agaaatccag tcagcgggct ggtggcatga gcaaaatgac tgcggctgcc
    14821 cggtccggac tgacgtgcca gcaggcctgc ttctaccgcc agacgaaatg ccttcgccat
    14881 gttgacggga tcgtccgcga cggcaattgc cgtattcact aacaccgcgt cggcccccat
    14941 ttccagcgcc tgcgcggcat ggctgggaac gccgatgcca gcatcgacaa ccaccggcac
    15001 tgtggcctgc tggataataa tctccagcat ggcgcgggtt tccagtccct gattcgagcc
    15061 aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac gtttacacaa
    15121 taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca gcgtttcggc
    15181 ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt gaatctctaa
    15241 ttttaaccag tttgtgccta acgcttcacg agccagatgg gcggcgaaaa tggcttcttc
    15301 cgctgttttc gccccggatg tatttggcag cagggtcaca cccgccgcga taagcggttc
    15361 gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg
    15421 gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc
    15481 ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatttcaa ccccctgcaa
    15541 taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgcc cactgctcac
    15601 gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct
    15661 ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg ttaaacagga
    15721 tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc
    15781 cactggctcg atttaccgtc gaacagtcgg agttctcccg caggtgtctc tataccgctt
    15841 aataacttaa tggcttccag tgcctgcaaa gtgcccataa ccccgaccac cgggccaacc
    15901 acgcccgccg tgcggcagtt gcgttctggc tcctggttat ctggccacag gcagcggtaa
    15961 cacccctgct cccagggcgg cgtcagtacc atcaactgac cgccaaatcc gaccgcgctg
    16021 gcggtgataa gcggcgtgtt gagtgccacg caggcggcat taatctcctg gcgagtcgcc
    16081 atattgtcgg tacagtcgag caccacatcg gcccgtgcaa ccgcatcttt taacgcctca
    16141 cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc
    16201 tgttggctga cctgcgattt cgggcgatcg atatcttcag tggtaaagag gatttgtcgt
    16261 tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc gacgccagcg
    16321 cccgccaggt acagcgcagc aggtgtaccc agcccgccca gaccgataat cagcacctgg
    16381 ctgtcgagca gtttttgctg cccgtccaga gcgatatcgt cgagcaggat ttggcggcta
    16441 taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca actgtgccgt
    16501 tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac tgccgacacc
    16561 cgttgctatc accgcaggcg cgcgtgccag actgataccg ccaatcgcca cggtgggata
    16621 atccgccagt cgctcaacat gccgtgccag ctgttccagc ccctgcggtg cagaaggcat
    16681 ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc gtgctgccag
    16741 cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg
    16801 gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc catacgcctg
    16861 atgcttgatc gccagccgcc agtaatcgtt gataaacaat cgcgcgttat agcggcggcc
    16921 cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat ctttgatgcg
    16981 tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta cgctgtccac
    17041 caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat acatcacgct
    17101 tcctccttac gcaggtagat ttctccgcct ctggcacgga agttctccga catatccgcc
    17161 attcccattt caatagtttg cgtggcggcg taatcacgca cttcctggct gattttcatc
    17221 gagcaaaatt tcggtccaca catggagcaa aaatgggcga ctttgccgga ctcttggggc
    17281 agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt aaactggtct
    17341 tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg cgcgcccgga
    17401 tgccctttcg ccaggtcagc ggcgtgggcg gcaatcttat aggtgataag cccctgctta
    17461 acatcttctt tattaggcag acccagatgc tcttttggcg ttacgtaaca gagcatcgcg
    17521 cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc atagcccggc
    17581 gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca gtgctctaac
    17641 tcctcggtca tattgcggcg gatcatctgc atcggcacgt ggcctgggcc ttcaatcatc
    17701 acctgcacgt catattccca ggcaattttg gtcagttcgc ccagcgtatg cagctcggca
    17761 aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc gcccagcgac
    17821 agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg atagaggaaa
    17881 ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg agagacgata
    17941 ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc atggatagtg
    18001 aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta
    18061 agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac tgtaccgatc
    18121 ggcaccgggc tgttacgcaa aatccactcg cgggtttcgt gaatatagcg accggtggag
    18181 agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc cacttcttct
    18241 tcgatggaag aggtgaccgc agagttgccg atattggcgt taacttttac caggaaattg
    18301 cgaccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat aatcgcacgt
    18361 ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag acgtgcgcca
    18421 aagctcattc ccggatgctg gtggcgtaaa acttcgctac ggatgcgctc gcggcccata
    18481 ttctcgcgga tggcgatgaa ttccatttcc ggtgtgatga tgccctggcg ggcgtagtgc
    18541 agttgggtga cgcggcgtcc tgctttggcg cgttttggtg ttagtacgcc gctaaaacgc
    18601 agttcgtcga ggccatcatc tgccagccgc gctttagtgt aatcggaact gcgcacggta
    18661 agttcttcgg tatcgccgcg cgcatcgatc cacggctggc gtagttttgc cagcccttgc
    18721 tgcacgttaa tggcaatctg cggatcacca tacgggccgg aggtgtcgta gaccggaatc
    18781 gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt cgggctaagc
    18841 tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt
    18901 gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt
    18961 tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag gacgtggctt
    19021 gtcagacgac ggatgaagca agagacgatc gccgcagggg cgatgcgata gcagattaac
    19081 tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa taaacggtct
    19141 cagccagtta aggcactccg acaagaaatt agccccgcaa atggggcatt gaatgtaaat
    19201 tacgcgttaa cagcgcagaa ctcaagcagg atgtttgacg cgggcggcgt caagcaccag
    19261 cagaatcagc ttatcttcca gcacaaagcg cgcttccagc gcctcgccga tgtcagataa
    19321 aacctgttga aactcaaggt aattatcatg atcgatggcg gtttccagac tggaatcgta
    19381 gtaatccata atctgttgag tattggcttc gagttgcggc caaatcttcg cggcgcgcgc
    19441 gagttgcccg ttgccttcca gcttatgaag aatgcgctca taaatactga aatgtccggc
    19501 agacaagtaa tcgaccaggc tctgacaaaa atcatcaagg gctttttcgt ttagcctcat
    19561 gtacgattct ttgccaggct taatgccaac cagattgtag taagccacga gcagatgctt
    19621 acgtacatgt agccagcgat caaccagttt gttacttcct ctgacgcgct ccgtcaggtt
    19681 atcgagctgg ttaagcatga ttgactccgc aagtttgtat tcaaaaactg ctcagtgaga
    19741 aatgtaaaaa ccatgttaaa catgccagtg atgcaaaggt agtgcaagag ctatggatcg
    19801 tataattgaa aaattagatc acggctggtg ggtcgtcagc catgaacaaa aattatggtt
    19861 gccgaaggga gaattgccat atggcgaagc ggcaaatttc gatcttgtgg gtcagcgcgc
    19921 actacaaatc ggcgaatggc agggggaacc tgtttggtta gtacaacagc agcggcgtca
    19981 cgatatgggg tcggtacgtc aggtcattga tctcgatgtt gggctgtttc aactggccgg
    20041 acgaggcgta caactggcgg agttttaccg atcgcataaa tactgtggtt actgcgggca
    20101 tgaaatgtat ccgagcaaaa ccgaatgggc gatgctgtgc agccattgcc gtgagcgtta
    20161 ctacccgcaa atcgccccct gcattattgt tgctatccgt cgcgatgatt cgatcctcct
    20221 cgcccagcat acccgccatc gtaacggtgt ccatacagta cttgccggat tcgtcgaagt
    20281 gggcgaaacc ctcgagcagg cagtcgcgcg ggaagtgatg gaagagagcg gaattaaagt
    20341 taaaaacttg cgttacgtga cttctcagcc gtggccgttt cctcagtctt taatgaccgc
    20401 gtttatggcg gaatatgaca gcggcgacat cgtgatcgac ccgaaagaat tgctcgaggc
    20461 gaactggtat cgctatgacg atttgccgtt actcccgccg cccggcaccg tagcgcgccg
    20521 tctgatagaa gatacggtgg cgatgtgtcg ggcagagtat gagtgatgat acactgaccg
    20581 cctgacgcac taaggaacag ccaaaatgac cgaacttaaa aacgatcgtt atctgcgggc
    20641 gctgctgcgc cagcccgttg atgtcactcc agtatggatg atgcgccagg cgggtcgcta
    20701 tctaccggaa tataaagcca cgcgcgccca ggcaggcgat tttatgtcgc tgtgcaaaaa
    20761 cgccgagctg gcgtgcgaag tgactttgca accgctgcgt cgctacccgc tggatgcggc
    20821 gatcctcttt tccgatatcc tcaccgtgcc ggacgcgatg gggttagggc tctattttga
    20881 agccggagaa ggtccgcgtt ttacctcgcc agtcacctgc aaagctgacg tcgataaact
    20941 gccaattccg gacccggaag atgaactggg ttacgtgatg aacgcggtgc gtaccattcg
    21001 tcgcgaactg aaaggcgaag tgccgctgat tggtttttcc ggcagcccgt ggacgctggc
    21061 gacctacatg gtggaaggcg gcagcagcaa agccttcacc gtgatcaaaa aaatgatgta
    21121 tgccgatccg caggcgctgc acgctctgct cgataaactg gcgaaaagcg tcactttgta
    21181 tctgaatgcg cagattaaag ccggtgctca ggctgtgatg attttcgaca cctggggcgg
    21241 cgtgcttacc gggcgcgatt atcaacagtt ctcgctctat tacatgcata aaattgttga
    21301 tggtttactg cgtgaaaacg acggtcgccg cgtgccagtc acgctgttta ccaaaggcgg
    21361 cggacagtgg ctggaagcga tggcagaaac cggttgcgat gcgctgggcc tcgactggac
    21421 aacggatatc gccgatgcgc gccgccgcgt gggcaataaa gtcgcgttgc agggcaatat
    21481 ggatccgtcg atgctgtacg cgccgccagc ccgcattgaa gaagaagtag cgactatact
    21541 tgcaggtttc ggtcacggcg aaggtcatgt ctttaacctt ggtcacggca ttcatcagga
    21601 tgtgccgcca gaacatgctg gcgtgttcgt ggaggcagtg catcgactgt ctgaacagta
    21661 tcaccgctaa ggagtgatta tggatctcgc gtcattacgc gctcaacaaa tcgaactggc
    21721 ttcttctgtg atccgcgagg atcgactcga taaagatcca ccggatctga tcgccggagc
    21781 cgatgtcggg tttgagcagg gcggagaagt gacgcgagcg gcgatggtgc tgctgaaata
    21841 tccctcgctt gagctggtgg agtataaagt ggcccgcatc gccaccacca tgccttacat
    21901 tccaggtttt ctttccttcc gcgaatatcc tgcgctgctg gcagcgtggg agatgctgtc
    21961 gcaaaagccg gatttagtgt ttgtcgatgg tcatgggatc tcgcatcccc gccgtcttgg
    22021 cgtcgccagc cattttggct tattggtgga tgtgccgacc attggcgtgg cgaaaaaacg
    22081 gctctgcggt aaattcgaac cgctctccag cgaaccgggc gcgctggccc cactgatgga
    22141 taaaggcgag cagctggcct gggtctggcg cagcaaagcg cgctgtaacc cgttgtttat
    22201 cgctaccggc catcgggtca gcgtggacag cgcgctggcg tgggtacaac gctgcatgaa
    22261 aggctatcgt ctgccggagc caacgcgctg ggcggacgcg gtggcctcgg aacgtccggc
    22321 gttcgtgcgc tatacagcaa atcagcccta attcaggtaa actgcggata atttccgtat
    22381 ttgagaactc aacatgttac aaaacccaat tcatctgcgt ctggagcgcc tggaaagctg
    22441 gcagcatgtc actttcatgg cttgcttatg cgaacgcatg taccccaatt acgccatgtt
    22501 ctgccagcaa accggttttg gtgatgggca aatttaccgt cgcattctcg atctcatctg
    22561 ggaaacgctg accgttaaag acgcaaaagt aaatttcgac agccaactgg agaaatttga
    22621 agaagcgatt ccttcagccg acgatttcga tctgtacggc gtttatccgg caatcgatgc
    22681 ctgcgtggcg ttaagtgaac tggtccattc gcgtttgagt ggtgaaacgc tcgaacacgc
    22741 ggtggaagtg agtaagacct ccatcacgac cgttgcgatg ctggaaatga ctcaggctgg
    22801 tcgcgaaatg agcgatgaag agcttaaaga aaacccagct gtagagcaag aatgggacat
    22861 tcagtgggaa atattccgac ttttagctga gtgcgaagaa cgcgatatcg agctgataaa
    22921 aggccttagg gcagacctgc gtgaggcggg tgatagcaat attggtataa tttttcagca
    22981 ataagaccag aaaacgtgat ttaacgcctg atttgtcgta cctggagtct tccctttcgc
    23041 cccccgtctg gtctacattt ggggggcgaa aaaaagtggc tatcggtgcg tgtatgcagg
    23101 agagtgctat tctggcattt ccgtcgcact cgatgcttag caagcgataa acacattgta
    23161 aggataactt atgaacaaga ctcaactgat tgatgtaatt gcagagaaag cagaactgtc
    23221 caaaacccag gctaaagctg ctctggagtc cactctggct gcaattactg agtctctgaa
    23281 agaaggcgat gctgtacaac tggttggttt cggtaccttc aaagtgaacc accgcgctga
    23341 gcgtactggc cgcaacccgc agaccggtaa agaaatcaaa attgccgcag ctaacgtacc
    23401 ggcatttgtt tctggcaagg cactgaaaga cgcagttaag taagattgcg tggcagtgaa
    23461 cagttttaac gaaggggtgg tttcacccct tttgtctttc tggcgtcgat cattgatgct
    23521 ggcaggtgcg ctgtttctca ctgcctgtag ccacaactct tcacttcctc cctttaccgc
    23581 cagtggattt gctgaagacc agggcgcggt acgcatctgg cgaaaagaca gcggcgataa
    23641 tgtgcatctg cttgccgtgt ttagcccgtg gcgcagtggc gataccacga cgcgagagta
    23701 tcgctggcag ggcgataacc tcacgctcat caatatcaat gtttacagca aaccgccggt
    23761 gaacattcgc gcgcgttttg acgatcgcgg tgatctgagc tttatgcaac gtgaatccga
    23821 tggggaaaag cagcagcttt ctaacgacca aatcgattta taccgttatc gtgctgctca
    23881 gatccgccag attagcgatg ccttacgtca ggggagagtc gtgctgcgcc aggggcgctg
    23941 gcatgcgatg gaacagaccg tgaccacctg cgaagggcaa accattaaac ctgatttaga
    24001 ttcgcaggcg atagcgcata tcgagcgccg ccagagccgc tcttctgttg atgtcagcgt
    24061 ggcatggctg gaagcgcccg aaggttcgca attactgtta gtggcaaact ctgatttctg
    24121 tcgctggcaa cccaacgaga aaacgttctg atttaccagt ggcccatacc catatgaccg
    24181 ccaccaccgc agccgccgta gcccatgccc attccggctc cgcgcggaat acccgcttca
    24241 gccatcgcga tatcccgttt cacccgtaac tcatctaacg actgacgcaa attctccatc
    24301 tctttggcga ccgcgttaat tttgctgcta tccggtgggt tcgcggctaa cagggcattg
    24361 tattcataac gcttcgtcac cagttgctgt tgcagtgcgc tgctttgagc gtaaaagtca
    24421 ttatggattt tctgccacgc tgtctgttgt tcgctggtca aaggcgcggc attttgctgc
    24481 cacataccgt gtccgccgtg agcaaatgca gatgtcgatc ccatcgccat tgctgaaagc
    24541 gccatcatta ccagggcaat tttcgtgttc cgtttcatgg ttaatcctcc agtggttgtc
    24601 ttacttcggg tattgcatct tccgtgccaa cgatgaaacg ctgatatgac gggtaatctg
    24661 gcatgataaa cgagtaaaaa tgactcgcct gctgcgggta gcgagtcatt tttactcatt
    24721 gaaacttaag cctttgtgtt acagcgcagg gtaagcgctg ataaaagatg gcatgatttc
    24781 tgctgtcaga aagggatgag caggcaaaga agaagatgcg ttttatgcaa cgttctaaag
    24841 actccttagc taaatggtta agcgcgatcc tccccgtggt cattgttggg ctggtagggt
    24901 tgtttgcggt gacggtgatt cgcgattatg ggcgcgagac tgccgccgcc agacaaacgc
    24961 tgctggaaaa aggcagtgta cttattcgcg ctcttgaatc cggctcgcgc gtcggcatgg
    25021 ggatgcgcat gcatcatgcg cagcagcagg ccttactgga agaaatggcc gggcagcctg
    25081 gagtacgttg gtttgcggtc acggatgaac aaggaacaat cgtgatgcat agcaactccg
    25141 gcatggtggg aaaacagctt tattccccgc aggaaatgca gcagttacat ccgggagatg
    25201 aagaagcgtg gcggcggatc gatagcgcag acggcgagcc tgttctggaa atttatcgcc
    25261 agtttcaacc gatgtttgct gctggaatgc accggatgcg ccatatgcag cagtatgccg
    25321 cgacaccaca agcaattttc atcgctttcg acgccagtaa tattgtgagt gccgaagatc
    25381 gtgagcagag aaacaccctg attatcctct tcgccctggc gacggtcttg ctggcaagcg
    25441 tattgtcatt cttctggtat cgccgctatc tgcgctcgcg ccagcttcta caagatgaaa
    25501 tgaagcgcaa agagaagcta gtggcgctgg ggcatcttgc ggcaggcgtt gcccacgaaa
    25561 tccgtaaccc actttcctcg attaaaggac tggcgaaata ctttgccgag cgcgcgcctg
    25621 cagggggaga agcgcatcaa ctggcgcagg tgatggcgaa agaggccgac cgtttaaacc
    25681 gcgtggtaag cgagctgctg gaactggtta agccaacgca tctggctttg caggcggtgg
    25741 atctcaacac gctgattaac cactcattac agctggtagg ccaggatgca aacagccggg
    25801 agatccagtt acgctttacc gccaacgaca cattaccgga aattcaggcc gatccggaca
    25861 ggctgactca ggtcctgttg aatctctatc tcaatgctat tcaggcgatt ggtcagcatg
    25921 gcgtgattag cgtgacggtc agcgaaagcg gcgcgggcgt gaaaatcagc gttaccgaca
    25981 gcggtaaggg aattgcggca gatcagcttg aagccatctt cactccgtac ttcaccacca
    26041 aagccgaagg caccggattg gggctggcgg tcgtgcataa tattgttgaa caacacggtg
    26101 gtacaattca ggtcgcaagc caggagggaa aaggctcaac gttcaccctc tggcttccgg
    26161 tcaatattac gcgtaaggac ccacaaggat gacgcacgat aatatcgata ttctggtggt
    26221 ggatgatgac attagccact gcactatttt gcaggcttta ctgcgcggct ggggctataa
    26281 cgtcgcgctg gcgaacagcg ggcgacaggc gctggagcag gtgcgggagc gggtttttga
    26341 tcttgtgctt tgcgatgtgc gaatggcaga gatggacggc attgccacgc taaaagagat
    26401 caaagcgtta aatccggcga ttccggtact gattatgacc gcatactcca gcgtcgagac
    26461 ggcggtagag gcactgaaaa ccggggcgct ggattatctc atcaagccgc tggatttcga
    26521 caccctgcag gcgacgctgg aaaaggcgct cgcgcatacg cacagtattg atgctgaaac
    26581 gcctgcggtg actgccagcc agttcggtat ggtcggtaaa agcccggcga tgcaacacct
    26641 gctcagtgaa atcgccctcg tcgcgccatc ggaagccacg gtgctgatcc acggcgattc
    26701 cggcaccggt aaagagctgg tcgccagggc gattcacgcc agtagcgcac gtagcgaaaa
    26761 accgctggta acgctcaact gtgtggcact caacgaatcc ttgctggaat ctgaattgtt
    26821 cggtcacgaa aaaggggcgt ttactggagc cgataaacgc cgggaagggc gctttgttga
    26881 ggcggacggc ggcacgctgt ttctcgatga aattggcgat atctcgccga tgatgcaggt
    26941 gcgtctgctg cgtgcgattc aggagcgcga agttcagcgt gtcggtagca accagactat
    27001 ctcggttgat gtccggctga ttgcggcgac ccatcgcgat cttgccgcag aggtgaatgc
    27061 cgggcgtttt cgccaggatc tctactatcg cctgaatgtg gtggcgattg aagtaccatc
    27121 gctgcggcaa cggcgggagg atatccctct gctggctagc cattttcttc agcgttttgc
    27181 cgagcgtaat cgcaaggcgg taaaaggctt tacgccacag gcgatggatt tgctgatcca
    27241 ctacgactgg ccgggaaaca ttcgtgaact ggaaaacgcg gtggaaaggt cggtggtgct
    27301 gctgaccggg gaatatattt ccgaacgcga gctgccgctg gcgattgcca gtacgccgat
    27361 cccgctgggg caaagtcagg atattcagcc gctggtggaa gtcgaaaaag aagtgattct
    27421 ggcggcactg gagaaaacgt gcggcaacaa aaccgaagcc gcccgtcagt tagggatcac
    27481 gcgcaaaacg ctattggcaa aactgtcgcg ttagttctgc tcgcgttcga tagcgcgcca
    27541 gccgatatct ttccggcaga agcagtcgtc ccagtgaata tcggtcatta aggcataagc
    27601 gcgtttctgc gcttctgcca cggtatgacc cagcgcggtg acgcacagta cgcgcccgcc
    27661 gctggtcact acctgctcgt tatccgccag tttcgtgccc gcgtggaaca ctttgccgcc
    27721 tgccacttct tccagcggca ggccgtggat cacgtcaccg gtgcggtaat cacccggata
    27781 tccacccgca gccatcacca cgccgagaga agcgcgttca tcccactctg acgttttctc
    27841 gtccagtttg ccttcacagg ccgccaggca gagctcaacc agatcggact tcatgcgcag
    27901 cataatcggc tgggtttctg gatcgccaaa gcggcagtta aattcgataa ccttcggatt
    27961 gccctgtttg tcgatcatca ggcccgcgta gagaaaaccg gtgtaggtgt tgccttccgc
    28021 cgccatgcct ttcacggttg gccagatgat acgttccatg gtgcgctgat gaacttcatc
    28081 ggttactacc ggcgcggggg agtaagcgcc catcccgccc gtgtttggtc cggtatcttt
    28141 atcgcctacg cgtttgtgat cctggctggt ggccatcggc agcacatgct cgccgtctac
    28201 catcacgata aagctcgctt cttcgccatc gaggaactct tcgataacga tgcgatgacc
    28261 cgcgtcgcca aaagcgttgc ccgccagcat atcgtgaaca gccgcttccg cttcttccag
    28321 cgtcatcgcc acgataacgc ctttcccggc agccagaccg tccgctttaa tgacgattgg
    28381 cgcgcctttc tcacgcagat acgccagcgc aggttctacc tcggtgaagt tctggtattc
    28441 cgccgtaggg atcttatggc gggccaggaa atctttggta aacgcttttg agccttccag
    28501 ttgggccgca cctgcggttg ggccgaagat tttcagcccg gcggcgcgga aggtatcgac
    28561 cacgcctttc accagcggcg cttccgggcc gacgatggtc agatcaatct tttcgttttg
    28621 tgcgaaatcc aacagcgccg ggatatcggt cacgccaata gcaacgtttt gcagcgcggg
    28681 ttccagtgca gtgcctgcat tacccggagc aacaaaaaca gtctcaacca gcggcgactg
    28741 ggccgctttc caggccagcg cgtgctcgcg cccgccgtta ccaataacta atactttcat
    28801 ctattgctcc attaatggcg gaagtggcgc atgtcggtga agagcatcgc aataccgtgc
    28861 tcgtcggcgg cggcaatcac ttcgtcatca cggatggaac cgccaggctg gattacgcag
    28921 gtcacgcccg cagcggcggc ggcatcaata ccgtcgcgga acgggaagaa cgcgtcagaa
    28981 gccatcgagg aacctttcac ttccaggcct tcatcggcag ctttaatacc ggcgattttc
    29041 gcggagtaca cgcggctcat ctggcccgcg ccaataccga tggtcatatt gtttttggca
    29101 tagacgatag cattggattt cacgaacttc gccactttcc agcagaacag cgcatcgcgc
    29161 agttcctgtt cggtcggctg acgttgggtg acgacgcgca gttcttccgc accgaccatc
    29221 cccaggtcac gatcctgaac cagcagaccg ccgttcacgc gtttgaaatc aagacccgga
    29281 acacgctcgc cccactgacc gcaggtcaga acgcgtacgt tctgcttggc ggcggtgatt
    29341 ttcagggctt cttcgctggc ggaaggcgca ataatcactt cgacaaactg acgagaaatg
    29401 atggcctgtg cggtttccgc atctagctcg cggttaaagg caatgatgcc gccgaatgcg
    29461 gaggttgggt cggttttgta cgcgcgatcg taagcatcaa gaatggaatt gccgatagct
    29521 acaccgcaag ggttggcgtg cttcacaatc acacatgccg gctcggcgaa ctctttcacg
    29581 cactccagcg ccgcatcggt atcggcgatg ttgttataag agagggcttt accctgaacc
    29641 tgggttgcgg tagcaacgga ggcttctttc acattctctt ctatatagaa ggcagcctgc
    29701 tggtggctgt tctcgccgta acgcatatcc tgcttcttaa tgaagttcag gttcagcgtg
    29761 cgtgggaagc gaccagcggc ttctttgctt tcaccgtggt acgccggaac catgctgccg
    29821 aagtagttgg caatcatgct gtcgtaggcg gcagtgtgtt cgaaggcttt gatggcgagg
    29881 tcgaaacggg ttgcaagcgt cagcgatcct tcgttgtcat ccatctcttt aataatggcg
    29941 tcgtagtcgc tgctcttcac cacgattgcg acatctttat ggttcttagc ggcggagcgc
    30001 accatggtcg ggccgccgat atcgatgttc tcaaccgcat cttccagcga gcaaccttca
    30061 cgggccacgg tctgggcgaa cggatacagg ttaacaacca ccatatcgat aggctggatc
    30121 tgatgttctt ccataatggc atcgtcctgg ccgcgacggc ccagaatgcc accatgtact
    30181 ttcggatgca gggtcttcac gcgtccatcc atcatctccg ggaaaccggt gtaatcggaa
    30241 acttcggtta ccggcagacc tttttctgct aacagacggg cagtgccccc tgtagacagc
    30301 agctccacac cgcgtgcgga aagtgcctgg gcgaattcga cgataccggc tttgtcagaa
    30361 acactgagca gagcgcggcg gactggacga cgttgttgca tggtaaatcc cctggatttg
    30421 actattacag agagcgttag ctgaattttt cgcgaaaaac tcagctaacg cccctaacgg
    30481 ggcatcctta tttttcgccc acattgtaac gaaaacgttt gcgcaacgct cgcgaatttt
    30541 tctctttcaa tggtgatcac aattttgaca gtggttaccg tgggaaaaat acagaaatta
    30601 cattgatgat tgtggataac tctgtgcgta aaaaggtata aagcggggtt ttgctgggga
    30661 atgcagcagt cagtcatttt tctgcaattt ttctattgcg gcctgcggag aactccctat
    30721 aatgcgcctc catcgacacg gcggatgtga atcacttcac acaaacagcc ggttcggttg
    30781 aagagaaaaa tcctgaaatt cagggttgac tctgaaagag gaaagcgtaa tatacgccac
    30841 ctcgcgacag tgcgctaaag cgcgtcgcaa ctgctcttta acaatttatc agacaatctg
    30901 tgtgggcact cgaagatacg gattcttaac gtcgcaagac gaaaaatgaa taccaagtct
    30961 caagagtgaa cacgtaattc attacgaagt ttaattcttt gagcatcaaa cttttaaatt
    31021 gaagagtttg atcatggctc agattgaacg ctggcggcag gcctaacaca tgcaa
//
LOCUS       DAZGLJ010000030        30322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000030 DAZGLJ010000000
VERSION     DAZGLJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.030"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            153..1736
                     /gene="yegH"
                     /locus_tag="ACHK49_001285"
     CDS             153..1736
                     /gene="yegH"
                     /locus_tag="ACHK49_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO9261960.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFCSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHGIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(2010..3863)
                     /gene="asmA"
                     /locus_tag="ACHK49_001286"
     CDS             complement(2010..3863)
                     /gene="asmA"
                     /locus_tag="ACHK49_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO9261961.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3885..4466)
                     /gene="dcd"
                     /locus_tag="ACHK49_001287"
     CDS             complement(3885..4466)
                     /gene="dcd"
                     /locus_tag="ACHK49_001287"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO9261962.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4558..5199)
                     /gene="udk"
                     /locus_tag="ACHK49_001288"
     CDS             complement(4558..5199)
                     /gene="udk"
                     /locus_tag="ACHK49_001288"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO9261963.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5517..8827
                     /gene="dgcE"
                     /locus_tag="ACHK49_001289"
                     /pseudo
     CDS             5517..8827
                     /gene="dgcE"
                     /locus_tag="ACHK49_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(8866..9723)
                     /gene="alkA"
                     /locus_tag="ACHK49_001290"
     CDS             complement(8866..9723)
                     /gene="alkA"
                     /locus_tag="ACHK49_001290"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO9261964.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            9857..11209
                     /gene="yegD"
                     /locus_tag="ACHK49_001291"
     CDS             9857..11209
                     /gene="yegD"
                     /locus_tag="ACHK49_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9261965.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRERLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11242..13161)
                     /gene="yegI"
                     /locus_tag="ACHK49_001292"
     CDS             complement(11242..13161)
                     /gene="yegI"
                     /locus_tag="ACHK49_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO9261966.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQA"
     gene            complement(13158..13919)
                     /gene="pphC"
                     /locus_tag="ACHK49_001293"
     CDS             complement(13158..13919)
                     /gene="pphC"
                     /locus_tag="ACHK49_001293"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFO9261967.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(13916..14575)
                     /gene="yegL"
                     /locus_tag="ACHK49_001294"
     CDS             complement(13916..14575)
                     /gene="yegL"
                     /locus_tag="ACHK49_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO9261968.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14796..14855)
                     /gene="ibsA"
                     /locus_tag="ACHK49_001295"
     CDS             complement(14796..14855)
                     /gene="ibsA"
                     /locus_tag="ACHK49_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO9261969.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15128..15184)
                     /locus_tag="ACHK49_001296"
     CDS             complement(15128..15184)
                     /locus_tag="ACHK49_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO9261970.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(15456..15512)
                     /locus_tag="ACHK49_001297"
     CDS             complement(15456..15512)
                     /locus_tag="ACHK49_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO9261971.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            15791..17038
                     /gene="mdtA"
                     /locus_tag="ACHK49_001298"
     CDS             15791..17038
                     /gene="mdtA"
                     /locus_tag="ACHK49_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO9261972.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17038..20160
                     /gene="mdtB"
                     /locus_tag="ACHK49_001299"
     CDS             17038..20160
                     /gene="mdtB"
                     /locus_tag="ACHK49_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO9261973.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVSTTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIASYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20161..23238
                     /gene="mdtC"
                     /locus_tag="ACHK49_001300"
     CDS             20161..23238
                     /gene="mdtC"
                     /locus_tag="ACHK49_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO9261974.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIVISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23239..24654
                     /gene="mdtD"
                     /locus_tag="ACHK49_001301"
     CDS             23239..24654
                     /gene="mdtD"
                     /locus_tag="ACHK49_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9261975.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            24651..26054
                     /gene="baeS"
                     /locus_tag="ACHK49_001302"
     CDS             24651..26054
                     /gene="baeS"
                     /locus_tag="ACHK49_001302"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO9261976.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26051..26773
                     /gene="baeR"
                     /locus_tag="ACHK49_001303"
     CDS             26051..26773
                     /gene="baeR"
                     /locus_tag="ACHK49_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO9261977.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            26964..27296
                     /gene="yegP"
                     /locus_tag="ACHK49_001304"
     CDS             26964..27296
                     /gene="yegP"
                     /locus_tag="ACHK49_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO9261978.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27505..27801
                     /locus_tag="ACHK49_001305"
     CDS             27505..27801
                     /locus_tag="ACHK49_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO9261979.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            27803..28099
                     /locus_tag="ACHK49_001306"
     CDS             27803..28099
                     /locus_tag="ACHK49_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9261980.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            28202..29563
                     /gene="yegQ"
                     /locus_tag="ACHK49_001307"
     CDS             28202..29563
                     /gene="yegQ"
                     /locus_tag="ACHK49_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO9261981.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29893..30210)
                     /gene="yegR"
                     /locus_tag="ACHK49_001308"
     CDS             complement(29893..30210)
                     /gene="yegR"
                     /locus_tag="ACHK49_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO9261982.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         7024 a         8071 c         7986 g         7241 t
ORIGIN      
        1 ttattattac cacttttaat tcagggataa tgtgaggtta ttaccctcaa ataatatgag
       61 ataaatagtg ctgcaacatt gcatttttgc cccgatttat ccatgatcga attatgacat
      121 ttgtcataca acgaataggt tttgtactta ctatggaatg gattgccgat ccgtctatct
      181 gggccgggtt aatcacgctg attgtgatcg aactggtcct cggcattgat aacctggtct
      241 ttattgccat cctcgccgaa aaactaccgc cgaagcagcg cgaccgcgca cgggttaccg
      301 ggctgctgct ggcgatgtta atgcgcctgt tactgctggc gtcaatctcc tggctggtca
      361 ccctgactca accgctgttc agcttctgct cgtttacctt tagcgcccgc gacttaatca
      421 tgctgtttgg tggtttcttc ctgttgttca aagccacgat ggagctgaac gaacggctgg
      481 aagggaaaga cagcaataat cccacacaac gcaaaggtgc gaagttctgg ggcgtggtga
      541 cgcaaattgt ggtactggac gccatcttct cactcgactc agtgattacc gccgtcggga
      601 tggtcgacca tttactggtc atgatggccg ccgtggttat cgctatcagc ctgatgttga
      661 tggccagcaa gccgttaacg caatttgtta acagtcaccc gaccatcgtt attctctgct
      721 taagcttcct gttgatgatt ggctttagcc tggtggcaga aggtttcggc ttcgtcattc
      781 cgaaaggcta cctgtacgct gccattggtt tctcggtgat gatcgaggcg ctcaatcagt
      841 tggctatctt taaccggcga cgttttcttt ccgctaacca gacgctgcgc cagcggacta
      901 ccgaagcggt aatgcgcctg cttagcgggc aaaaagaaga tgcggaactg gacgccgaaa
      961 ccgcgtccat gctggtggat catggtaacc agcagatctt taatccgcag gaacggcgga
     1021 tgattgagcg ggtacttaat cttaaccagc gtactgtcag cagcattatg acgtcgcgcc
     1081 acgatattga gcatatcgat ctcaacgcgc cggaagaaga aatccgccag ttgctggagc
     1141 gaaatcagca tacgcggctg gtggttaccg acggtgatga cgcagaagat ttgctcggtg
     1201 ttgttcacgt tatcgacctg ctacaacagt cactgcgcgg cgaaccgctc aacctgcggg
     1261 tgttgattcg ccagccgctg gtgttcccgg aaaccttgcc gttgttacct gccctggagc
     1321 agttccgtaa tgcccgcacg cactttgctt ttgtagtgga tgagtttggc tcggtggaag
     1381 ggattgtgac attaagtgac gtcactgaaa ccattgccgg taacttaccg aacgaagtgg
     1441 aagagatcga cgcccgccat ggtattcaga agaatgcgga cggttcctgg acggcgaatg
     1501 gtcatatgcc gctggaagat ctggtgcaat atgtgccgct gccgctggat gaaaaacgtg
     1561 aatatcacac cattgccggg ctgttgatgg aatatttaca gcgtattcca aaacctggcg
     1621 aagaagttca ggtgggggat tatttgctta aaacgttgca ggtggaaagc catcgcgtgc
     1681 agaaggtgca gattattccg ctgcataagg atggcgagtt ggagtacgag gtgtgatggt
     1741 atcggtcatt ttacccgata gccgaaagga ataagatgag ggaaccgcgc cagcggcgat
     1801 ttaccgccgg gagcccgggt tgccagggtg gtggcgattg agccaccctg gcacgttcac
     1861 cggcttgagc gttccatagt agtgaggaac atcaagtgaa cggaacagcc ttcgatctta
     1921 catattcaga atataatggt gccgccgcga acgcctgatg cgacgcttta cgcgtcttat
     1981 caggcctaca ggacaggtca cctggtaacc tacatcttct ccagcaactt cttcacatct
     2041 ttgccattgc gggaatcttt attccgctcg gcccagtcat tcaggcgacg tttcgcttcg
     2101 tcctgtagat gtttgcgcag taactgatcc acttgcagac tgtaattgag ttgctgccag
     2161 ttgccataaa cccgcagcgg tactggcgtt tctttgagga aatcaatcag tttgctttcc
     2221 ccgttccagc cacccacgac ccgaatatca aactgggtgt cgcaggtttg atctgccaga
     2281 ttcaacatgc cttcccctgt cagcgccagc acaggcgatt gaccttgcat gtcgtttaac
     2341 gtcacgacgc catccttcaa cgtcaaatcg gtggtaaagc ggtcaagacg cgttacgtta
     2401 tcgaagtttt cagcggcctt cacatcacca ccattacgtt ctaccgcttg ctgaatcatc
     2461 tgctggaagt tcatcccttc catgcgcgtg tcggtcattt cgacatgtgc ctgtccttgc
     2521 cagttgtggc ggaatgcgtc ggcatctatg tcagcaccgg agaaatcacc agccagtgac
     2581 atttttccgg tcaacgaaat cggatagtta aacgccttca gaatggtacc aatctcaacg
     2641 ttttccagcc gtggctggaa gtttatccgc ggatttattg atgtcgcgtc cagcgtgccc
     2701 ggcagtgaaa cctgtccacc gttaagcttg ccctgcagtt gagtaatttc cagcaaaccc
     2761 gacttgttgg tcatttgcgt ggcaacatct gtaaaattca ttccgcgcca gcgcacgtta
     2821 ctggcctgca acaaaatatc agccgtaaag ccttgcagtc cctgatatgc cggttcatca
     2881 atacgcgaag aaatgaccgg gcgcggcaat gttgattggc tctgcccctg ctgcgcggca
     2941 ccgttttcac cattcgctgt ttcattaagc gggatgaggt tgtcaagatt cagttgcggg
     3001 aattgcagtc tcagctgcca ttcaggtttc tctgtcagcg tgacctgtgc ttgcccgctc
     3061 agcgtgctgt cattggcggt caagctgatt tggttaaatg aaaggcgttt atgtgactcc
     3121 tgccactggg cctggaaact cccctgcccc tgaatacctt gttttggtaa atctgcaccc
     3181 tgcaactgcc agttaatttg ttcaatagcc gccgttaaat catgcggata atcagaagca
     3241 tctaccgtac cgttaaggga tatcgtgaga tcgcgctgat cgcgattaac ccgcccggag
     3301 aactcaaatg agccacgatg ttggggatct tgttccattt gcaggcggat attgcggatt
     3361 gtcacctgct cgtcatcttc atgctggaac accagcacgc tgtccgccac cttaagactg
     3421 gatatatcaa acgaccatcc gcgatcgtct gacagatccg gcaaggtatt gtcgcgcggt
     3481 gcaaccggag cgtcttcact gcgcaccgct tccgtctgcg gcgtcagttg gatcactgcc
     3541 ccttttagca tcacctgctt aacgctcagt tgatgactca gtagtggtaa aagcgccacg
     3601 tccagacgca tgttgtcggc gcgaaccagt ggctggcttg cgccctgggc ggtgagagac
     3661 attcgcccgg agaggatact aagctgcggc cagacgtgcc aacgcagtgg cccgtcgagc
     3721 tgcaattgat aaccgctacg tgcagcaact tgcttgacca tatagtcgcg gaaatcattc
     3781 ggattcacca gcaacactaa cgcagataac ccggccacca gcacgaccag gagtatcatc
     3841 agcgtcgtca gaaatcgtct catggtatcc tcaatgggcc tgaattagtc tttatcgatt
     3901 cggctggcta ccgcgccctg ctggttgcga tatttcgcat cttcacggcg gttgtaaggt
     3961 cgcgccgccg ggccggaaag cggctcaaag ctcagcgcac caattaacat gcccggacgc
     4021 agcgccagcg gcagcttacc ggagttgtag aactccagca caatgcaacc agaccagccc
     4081 ggatcgatac ggtgcgcggt gacgtgcacc atcagcccca gacgcgccaa agaggaacgc
     4141 ccgtccagcc agcccaccag atcggctggc agcgtcaccg actccagcgt caccgccagc
     4201 gccagctctc ctgggtgaag atagaacgcc tcgccctcgt cgagaacgat ctcatcgctc
     4261 atcacgcggt caagcgcggc gctcacttca tctttgggac cgctcagatc gataaacgct
     4321 gccgtgtgac cacggaaggt acgaaattta ttgcccaggc gtacatccac cgtcgcgccg
     4381 ttaatacgct ccactggcgg acgtgggttg atcgacaaac ggccttcatc aagccaggct
     4441 tcaatatctc ggtcacacag acgcatggca ttttctcctt tcgcgcatca ctcccttaac
     4501 gccaattacg tcaagggcat accaggttat cactgaacgg tacacaattt atcaagctta
     4561 ttcaaagaac tgacttattt tcgctttcaa tatatcgatc gcgatgcggt ttttcccgcc
     4621 gcgcggcacg ataatgtccg catattgttt agaaggctca atgaattgca ggaacatcgg
     4681 gcgcacggtt ttttgatatt gcgccatcac ggaatccatt gaacgcccac gctcgttaac
     4741 gtcacgcttg atgcggcgca tcaggcagat atccagcggg gtatcaacga aaatggagaa
     4801 gttaagttcg tcacgcaaac gcgcatccgt cagcaacaaa atgccttcga gaatgatgac
     4861 cttcttcggc tcaaccgtca ccgtttcttt catacgcgta tgttcaacat agctgtaaac
     4921 cggcaggtca attgccgagc cgcgtttcaa cgcttgtaaa tgctcaagca acaggctgtg
     4981 atccatcgcg ctgggatggt cgtagttggt cttaacgcgt tcttccatcg acagatggct
     5041 ttgatctttg taatagcagt cttcgggaat tacgccgatg tgttcatcac cgacttgctc
     5101 acgtaattca cgataaaggg tactggcaat aagactcttg ccggaagccg atgcgccagc
     5161 gataccgata atgacgcact gatgagactg atcagtcata tatttagcga cctgattaac
     5221 ctggatgtta ggaagggggc gacgaagcgc caaacgcggc aattataggg atttcatccg
     5281 cctgatacca gtcgaatagc gctgccgcgc gctcagagtt aattgttgac gaagaattcc
     5341 cggtggcaaa ttacgttgat cagttttata caaggtaaaa aatgttatac gcagttgcgc
     5401 aaattatccg cctttacgtc actttatgag caattcgcat ataaaatgta aaacttttgt
     5461 actagcataa acacagaaac gaatactggc gacctggtct tgcggataaa gcggtaatga
     5521 gcaaacaatc acagcatgta ttaattgccc tgccccaccc gctgcttcac ctggtcagtt
     5581 taggtttagt ctcgtttatc tttacccttt tctcgcttga gctttcgcag tttggcaccc
     5641 aactcgcccc actgtggttc ccgacgtcca tcatgatggt ggcgttttat cgccatgccg
     5701 ggcgcatgtg gccgggaatt gcgctgagct gctcgctggg aaatatcgcc gcatccatcc
     5761 tgcttttttc caccagctcg ctgaacatga cctggacgac catcaatatt gttgaagccg
     5821 tggtcggggc agtgctgcta cgtaaattgc tgccgtggta taaccccttg caaaatctgg
     5881 ctgactggct gcgtctggca ttcggcagcg ccattgttcc gcctctgcta gggggtgttc
     5941 tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata tgggtactgt
     6001 cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa ccacactatc
     6061 tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta gccatcacac
     6121 tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc attattgtgc
     6181 tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc ttccttacca
     6241 cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct acgccgcgta
     6301 catacctgat gagccatatg ccgtggctac cgtttttgct gatcctgctg cccgccaaca
     6361 tcatgacgat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc gaaagcgaaa
     6421 cccgttttcg gaacgccatg gaatattccg ctatcggtat ggcgttagtg ggcaccgagg
     6481 gacaatggct gcaaaccaac aaagcgctct gccagtttct cgggtacagt caggaagagc
     6541 tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa gatctccaac
     6601 aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa cgctactaca
     6661 accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc cacacggatg
     6721 gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa cgcaccgaac
     6781 aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc gggattggca
     6841 tctgggagtg ggagttgaag ccgaatattt ttagctggga taagcggatg ttcgagctgt
     6901 atgaaattcc tccacatatc aaaccgaact ggcaggtgtg gtacgagtgc gtgctgccgg
     6961 aagatcgcca gcatgccgaa aaagtgattc gttgcaatca cgctcgccct ttaaactgga
     7021 atttcgcatt accgtaaaag acggtattcg ccatatccgc gccctcgcca accgggtact
     7081 gaataaagaa ggcgaagtcg aacgcctgct cggcattaat atggatatga ctgaggttaa
     7141 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacataacgc tggactccat
     7201 cggcgaagcc gtggtctgta ttgatatggc aatgaaaatt acctttatga atccagtggc
     7261 ggagaagatg agcggctgga cgcaggaaga agcattaggt gttccgctcc tgacggtgtt
     7321 gcatattacc tttggcgaca atggtcccct gatggagaac atttacagtg ccgacacctc
     7381 acgttccgcg attgaacaag atgtggtgtt gcactgccgg agcggcggca gctacgacgt
     7441 gcattacagt attacgccgt taagtactct ggacggcagc aatattggtt cggttctggt
     7501 gattcaggac gttaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
     7561 tgatgcactg acgcatctcg ccaatcgcgc cagttttgag aagcaactgc gtatgctgct
     7621 gcaaacggta aacagtacac atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
     7681 taaagcggtg aatgacagcg ccgggcatgc ggcgggcgac gctttgctgc gcgaactggc
     7741 gtcgttgatg ctgagtatgc tgcgttccag cgacgtgctg gcgcgactcg gtggggatga
     7801 atttggtctg ctgctaccag actgtaatgt tgaaagcgcg cgttttatcg ctacacgcat
     7861 tatcagtgcc gtgaatgact atcactttat atgggaaggc cgtgtgcatc gggtaggtgc
     7921 cagtgccggg attaccttga ttgatgacaa caatcatcag gcggcagaag tgatgtcgca
     7981 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
     8041 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
     8101 gatgattaaa gagaatcagt tgatgatgat ggcccacggt gtcgcttcgc cgcggatccc
     8161 ggaagcgcgt aatttgtggc tgatttcgct taaactctgg agttgcgaag gcgagattat
     8221 tgatgaacaa aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
     8281 acgggtattc cacgaatttt tccagctggc cgcaaaagcg gttgccagta aaggcataag
     8341 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
     8401 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggcaga
     8461 cgcgatttta gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt
     8521 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
     8581 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
     8641 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
     8701 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
     8761 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
     8821 taactgaggg agcgtttgcc cattgcctga tgcgacgcat caggcctaca attcgtccgt
     8881 tccgtctggt tgccagcctt ccgtatacca gatatgcaac agcgcataag aacgccaggg
     8941 cttccagcgc tcggcataac ggcggatttg tgccggtgtc atccccggaa atcgctgttt
     9001 aatcagataa tcatccggca gaaaaacatc tttcgcctgc cagccacgca aagcgaaata
     9061 attcgccgtc cagcgcccga tacccggaaa agtttgcagc gttttcatcg cctgctccac
     9121 atcgcccgga attgtcattg gtaagctgcc ctccagcgcc gcatttgcca gatgaatcag
     9181 tgcctctgcc cgtttcaacg gcatgcctaa cgcttttaat gcctgcgggt cggctgctgc
     9241 cagccgctga ggcgtgggga agcagatata ctccggaaaa tcatccagcc gttcgccata
     9301 aagctgtaca actttggcag tcaattttgc cgccatcgcc acgctcacca gttggcctaa
     9361 aatcgcccgc acaccctgct caaaagcatc aatacagccg ggtaaacgca atccgggccg
     9421 cgccgcgcct aacttgccca acgcaccgtt aacaatctgc gggttacatt gcagatcaaa
     9481 caggcggctc attttcgcca gacactcagc ggcaacgggt tctaaacctg cacttaaatt
     9541 tatgtgcaga gtatggcggg ctatatccgg aatagcagtc accacgccgc gatattcgcc
     9601 caccgccaga ctacgggcat aataatcctc agcgaccgtt tccacaccac tcactgcacg
     9661 ggcggcgaga aatcccaaca tccacgacca gtcatacggc ggctgccagt tcagggtata
     9721 catcgcatct ccttattcat ccgctttcag cataaacgtt attcagacgc tacgctttgc
     9781 tttcatattc cggttgtcgc gacagcaaca tttcgctaaa gtcacgcccc ttcttcaccg
     9841 gcatggggat taattcgtgt ttattggttt tgattacggt acagcaaact gttcagtggc
     9901 ggtcatgcgt gacggtaaac cgcaattact gaaaatggaa aacgacagca cgctgctgcc
     9961 ttcaatgctt tgcgcgccaa cgcgtgaatc ggtaagcgaa tggctgtacc gccatcatga
    10021 tgttccggca gacgacgatg aaacgcaggc gctgttacgt cgggcgattc gttataaccg
    10081 cgaagaagat atcgatgtta cggcgaaaag cgtgcagttc ggtctttcct cactggcaca
    10141 gtacattgat gatccagaag aagtgtggtt tgtgaaatca ccaaaatcgt tcctcggtgc
    10201 cagcggctta aaaccgcagc aggtagcgct gtttgaggat ctggtctgcg caatgatgtt
    10261 gcacattcgc cagcaggcgc aggcgcagct gccagaagcg attactcagg cggtgattgg
    10321 tcgcccgatc aacttccagg ggctgggcgg cgatgaagca aacgcccagg cgcaagggat
    10381 tctggaacgc gcggcgaagc gtgccggatt cagggacgtg gtattccagt acgagccggt
    10441 cgcggctggg ctggattacg aagccacctt gcaggaagaa aaacgggtgc tggtggtgga
    10501 tatcggcggt ggtacgactg actgttcatt gctgctgatg gggccgcagt ggcgttcgcg
    10561 tctcgatcgt gaagccagcc tgctgggtca cagtggttgc cgtattggcg gtaacgatct
    10621 ggacatcgca ctggcgttta aaaacctgat gccgttactg ggcatgggtg gcgaaaccga
    10681 aaaaggcatc gccctgccga ttctgccgtg gtggaatgcg gttgccatca acgacgtacc
    10741 tgcgcagagt gatttctaca gtagtgccaa cggtcgtctg cttaacgatc tggtacgcga
    10801 tgcccgcgaa ccggaaaaag tggccctgct acagaaagtc tggcgtgagc gtttaagcta
    10861 tcgcctggtg cgtagcgcag aagagtgcaa aattgctctt tcaagcgtag cggaaacccg
    10921 cgcctcactg ccgtttatca gcgatgaact ggctacgctg attagccagc aagggctgga
    10981 aagcgccctc agccagccac tggcgcggat tctggaacag gtgcaactgg ccctggataa
    11041 cgcccaggaa aaaccggacg ttatctacct gaccggcggt agcgcccgtt cgccgctgat
    11101 taaaaaagcg ctggcagaac agttgccggg aattccgatt gcaggcggcg atgactttgg
    11161 ctccgtcacc gccgggctgg cacgctgggc ggaagtggtg tttcgttaaa agaaaatggc
    11221 tcagcagcgg cttaaatctg cctacgcctg agccagtttt tctgccgcct gacgtaaagg
    11281 ttccatcaac ggcatggtcc gtgctggagc atgaaggcgg aatcgctgca actctgtcgc
    11341 gccgacagtc aacgtcgatt ccagccgatg tcgtttggcg gtcattttcg ccattaccgc
    11401 ttgtctgtct gctggcgtta tcgcttcatt cggcctgaac acaaaacggc gttcacagct
    11461 cgctttccag tcgataaccg cctgggtcag atggtcgcca aaccctttca cttgcttaac
    11521 gccacgacgg gtaacatctg cggctgtttc aataccaaaa gaccgtaacg ccgctttacg
    11581 cgcagggcca acgccgggaa tagaggcaac atcaataaaa aatccctcca gaaacttctg
    11641 cttctgccgt tcccttgcag tgtcatgaag tgctgccaga gcgcgtttct cttcttccgg
    11701 taacccgaga atttcgtcct tcattttttc gagcatcgcc cgtttggcga taaaaccttc
    11761 cagcccgccc aactgttgga tctggctgac taaatgctca tagtccattt tcgcgcgatt
    11821 aaatgcctca cggcgttgct ggatttctgc tttgtacgct ttgcttgtca gactgccaat
    11881 aatccagata gccgacagca caggaaccaa aataatataa cgcggttctg cctgaaggcc
    11941 gcaaagcaac agcgataacg ctgacagtgc gatctcaatc agaatgatgt attcgcgccg
    12001 taacaggcct aaaggaagcg gcctgccagt cggttggaaa tgatcgggta atggcaattg
    12061 caatgctggc ggtgctactg acgccatcac catcgcccag actttcgcca gcacaaaatc
    12121 accgccggtg gtaatgacct cttcgccgag atcaataaaa tagataacgc cttgattatc
    12181 cagcgcacac cacgggcagt cggtcaaatg agcgggataa acatgcattg ccgaaacggg
    12241 acatttcttt aactgttggc gcagagaatc aagtgccgct acccacgctt tagccgtcgg
    12301 acgcccggtt gccacgccac tttccgtgaa tgcctgctga aacatggctt caacatcgcc
    12361 cggtaacatc gatagcggaa tcgatcgtgg cggcggtttt aaaccacgtc gctgattatc
    12421 tgacgcgtag gcataacgga aatgggtaat atccgtctcc agcgcattac ccgcatcaga
    12481 gataagcggc acaccagaat aaggatgccg gccaccaaac aagacgtgaa aaatcagcaa
    12541 cgcaaggcca aaattatcgt gattttctgt gcgttcaaag ccgacaaatg acgacaaggt
    12601 ttgtagctct ggcggcgtaa aatgcgacac gccgacttcg cataaatgca gtgtgccatt
    12661 ggcgttaatc tgaaaggagt cgctatcaat cagtaccact ttgctgtcgc gacctaccat
    12721 aaaactgttc tggtttacgt cacccacgac gtgcccgtgc tcgtgaaccg tagcaaaaga
    12781 tgaagcaata ttgcgcgcaa catagagtag aaaatcccac gcacaatgag ggtaacgttg
    12841 acgacgatgt gccgggctat agatcatatg aatcggttct ttaccagaaa cttttggcat
    12901 cataaaaccg ataacttttc cgcctcgtcc accgtgaagc gttgcctgcg gccaggcgac
    12961 ataattcaac aattgcgcgt cagctgtcgc agccataaag gcaagtttgt cctgttttaa
    13021 ggcgggtggc ggcgtgtgat aaatcttggc gacgctatcg acaaactcat tgatatcata
    13081 aaccgcgcct tcaccgcctt tgcccagttc acggcctggc gtaacgcact caccagtagc
    13141 agtatataaa gtgggtttca tggtaaccac agcgccagtg ccaacgtttt atcatcatcc
    13201 gtacgctcgt ttaccgccgg gctggacaaa aactgcttta acaattcagg taataaatcg
    13261 agttgttcct gcgttgctga cgccagtcca ttgaaaaacg gggtaaagaa aggtacatga
    13321 ggagaattat ccagcatatt taacgccagt cgctggatac catccgtaaa tgctgcaact
    13381 ttatgcacac gctcagtgct ggtgaaagtt tccagtctgg aaacggcatc ttcatcggta
    13441 atgaagtgcg tcatattggc atattcaccc accatcggcg taagcggcaa ttgcagaccg
    13501 tgaccaaaat ccaccaccac gccgccatcg ccaatctgca taatcaaggt gccattagcc
    13561 gatgaaatca atcccagaaa cgtgcaggca aaatcgcgaa ctgctaattc tttggcttcc
    13621 gcctcggcaa aaagtcgctg acgaacggtc agcacaatat ccgtggcgag gatatcatta
    13681 agccccaatt ccccaccctg cactttttgc gacatataag ccatcgcttc attgacggcg
    13741 agcgtcgcgc cttctcctcc ctgcgaaacg ctacctgcgc catcagcaag gaacatcacc
    13801 agtaatggct gctggtcatt tagccaggca acctgcatct ggcaggcatc ctggcaaggc
    13861 aagtccgcgc tgatgtgcga tgttccaaca gcggatgcat aaaccagacg ccagctcaca
    13921 ctgacgtcca gccttttggc gcttccagca caacttccgt tcccggcgtg gagcgggaaa
    13981 ccgaacgcag cgaactggat aaccagctaa acagttcacg gaattgtaat ccttgcagag
    14041 gcaaaggctg acgaacgctg atttgtgcca gagtcttcat atccgccccc tgaacaccga
    14101 tggaaaagaa agcaaacctt ttatcttctt ccccccgaaa cactttgttg gctgcagcct
    14161 gccactcatc ggttggtgca ccatcggtaa tcaggaaaat ccacgggcga taatacgaaa
    14221 taccatttgc acgatactca cgtttccgct cctctaccat atccagggct ttggtaatgg
    14281 ccgcacccat tggtgtatcg ccctgggcaa acaggatggg cggaaaaaaa ttagcggcgc
    14341 tggtaaaggg ttgttccaca tgcaccgggc cgaacgtcac aatgccgagt tccactcttt
    14401 ttaaagccag cggatcggca agcagttcat cacgaaaggt caccaatccg gcgttaagtt
    14461 cattaatagg tctgccgctc atcgagccag aaacatccag cagcaaaatg caggggcaac
    14521 gtggttccgg gttactggca aaatcgctgg tggcaaacgt gatttgttcg ctcataagaa
    14581 atcctttctt agagaattat cgaggtccgg tatcataccg actgcaataa tgaaataaga
    14641 gcaaacgaag aaactttcag aattaggccc ggcggcgcag aaaattttgc tattcgcctc
    14701 gcaagaacgg agagccgtcg attgacacta agggcggagt gacataattt caggagtgag
    14761 ggttagggag aggtttcccc ctccccctgg tgttcttagt aagcctggaa gctaatcact
    14821 aagagtatca ccagtatgat gacgtgcttc atcataaccc tttccttatt aaaagccctc
    14881 ttctccggga gaggctttcc cgtttcagcg tcccgctgaa atcatcggct tacctccttt
    14941 cgccctgaat gcagtctatc gctaacgcgt gaataaaata gtttcctctg tgattattgc
    15001 gtaacgactc gcaaagaata tttgctggca ggatcgcaga ctacaaagcc tgcggattga
    15061 caatcttatc gtgaaggcat actttcagga gtgagggtag agcggggttt cccccgccct
    15121 ggtagtctta gtaagcgggg aagcttatga ctaagagcac cacgatgatg agtagcttca
    15181 tcatgaccct ttccttattt aaggcccctt cctcgggagg ggctttcccg tttcagcgtc
    15241 ccgctgaaat cgtcggctta cctcctttcg ccatgcactt agtctatcgc tgacttgcag
    15301 ataaaatagt ttccagtgtg attattgcgt aacgtctcgc aaagaatttt tgctggcagg
    15361 atcgcagacg gcaaagcctg cggattgaca atcttatcgt gaaggcatac tttcaggagt
    15421 gagggtagag cggggtttcc cccgccctgg tagtcttagt atgctgggta gctaatcact
    15481 aagagcacca cgatgatgag tagcttcatc atgacccttt ccttatttaa ggccccttcc
    15541 tcgggagggg ctttcccgtt tcagcatccg gctgaaatct gcggcttacc tcctttcgcc
    15601 ctgtatgcag tctatcgcta acgcgtagat aaaatagttt cctgtgttat tactggatgc
    15661 gtgctcgcaa atgtgcccgc cattcagacg attcccgata gtgtttcata attcctccat
    15721 ttttctccct tattggctgg ctacactagt atcattccgc gaaacgtttc aggaagagaa
    15781 actcttaacg atgaaaggca gttataaatc ccgttgggta atcgtaatcg tggtggttat
    15841 cgccgccatc gccgcattct ggttctggca aggccgcaat gactcccgga gtgcagcccc
    15901 aggggcgacg aaacaagcgc agcaatcgcc agcgggtggt cgccgtggta tgcgttccgg
    15961 cccattagcc ccggttcagg cggcgaccgc cgtagaacag gcagttccgc gttacctcac
    16021 cgggcttggc accattatcg ccgctaatac tgttacggtg cgcagccgcg tggacggtca
    16081 actgatggcg ttacatttcc aggaaggcca gcaggtcaaa gcaggcgatt tactggcaga
    16141 aattgacccc agccagttca aagttgcatt agcacaaacc cagggccaac tggcaaaaga
    16201 taaagccacg cttgctaacg cccgccgtga tctggcgcgt tatcagcaac tggcaaaaac
    16261 caatctcgtc tcccgtcagg agctggatgc ccaacaggcg ctggtcagtg aaaccgaagg
    16321 caccattaag gctgatgaag caagcgtcgc cagcgcgcag ttgcaactcg actggagccg
    16381 catcaccgca ccagtcgatg gtcgcgttgg tctcaagcag gttgatgttg gtaaccaaat
    16441 ctccagtggt gataccaccg gaattgtggt gatcacccag acgcatccta tcgatttagt
    16501 ctttaccctg ccggaaagcg atatcgctac tgtagtgcag gcgcaaaaag ccggaaaacc
    16561 gctggtggta gaagcctggg atcgcaccaa ttcgaagaaa ttaagtgaag gcacgctgtt
    16621 aagcctcgat aaccaaatcg atgccacaac cggtacgatt aaagtgaaag cacgctttaa
    16681 taatcaggat gatgcgctgt ttcccaatca gtttgttaac gcgcgcatgt tagtcgacac
    16741 cgaacaaaac gccgtagtga tcccaacagc cgccctgcaa atgggcaatg aaggccattt
    16801 tgtctgggtg ctgaatagcg aaaacaaggt cagcaaacat ctggtgacgc cgggcattca
    16861 ggacagtcag aaagtggtga tccgtgcagg tatttctgcg ggcgatcgcg tggtgacaga
    16921 cggcattgat cgcctgaccg aaggggcgaa agtggaagtg gtggaagccc agagcgccac
    16981 cactccggaa gagaaagcca ccagccgcga atacgcgaaa aaaggagcac gctcctgatg
    17041 caggtgttac ccccgagcag cacaggcggc ccgtcgcgcc tgtttattat gcgtcctgtc
    17101 tccaccacgc tgctgatggt ggcgatctta ctcgccggga ttatcggtta tcgcgccctg
    17161 cccgtttcgg cgctgccgga agtggactat ccgaccattc aggtggtcac gctctaccca
    17221 ggtgccagcc cggatgtcat gacctctgcc gttaccgcgc cgctcgaacg ccagttcggg
    17281 cagatgtctg gcctgaaaca gatgtcgtcg caaagttccg gcggtgcgtc agttatcact
    17341 ttgcagttcc agctaacatt accgctcgat gtcgccgagc aggaagtgca ggccgcgatt
    17401 aacgctgcga ccaacttgtt gccgagcgat ctgcctaacc cgccggttta cagcaaagtg
    17461 aacccggcag atccgccgat catgacgctc gccgtcacct caaccgccat gccgatgacg
    17521 caagtggaag atatggtgga aacccgcgtc gcgcagaaaa tctcgcagat ttccggcgtc
    17581 ggcctggtga cgctttccgg cggtcagcgt ccggctgttc gcgtcaaact taacgctcag
    17641 gcgattgccg ccctcggcct gaccagcgaa accgtgcgca ccgccattac cggcgctaac
    17701 gttaactcgg caaaaggtag cctcgacggc ccttcccgtg cggtcacgct ttccgcgaac
    17761 gaccagatgc aatccgccga agagtatcgc cagctaatca tcgcctacca gaacggcgcg
    17821 ccaattcgtc tgggcgatgt cgcaactgta gagcaaggtg cagaaaacag ctggctcggc
    17881 gcgtgggcga acaaagaaca ggccattgtg atgaatgttc agcgccagcc cggtgctaac
    17941 attatctcca ccgccgacag cattcggcag atgctgccac agctcactga gagtctgccg
    18001 aaatcggtga aggtgacagt gctttccgat cgcaccacca atatccgcgc atccgtcgat
    18061 gatactcagt ttgaattgat gatggctatc gcgctggtag tcatgattat ctacctgttt
    18121 ttgcgcaata ttccggcgac catcattccc ggtgttgctg taccgctgtc gttaatcggc
    18181 actttcgcgg ttatggtgtt tctcgatttt tcaatcaata acctgacact gatggcgtta
    18241 actatcgcca ccggattcgt ggtcgatgac gccatcgtgg tgatcgaaaa catttcccgc
    18301 tatatcgaaa aaggcgaaaa accgttggcg gcggcgctca agggcgcagg tgaaatcggc
    18361 tttaccatta tctcgctgac cttctcactg attgcggtgt tgatcccact gctgtttatg
    18421 ggcgatatcg tcgggcgact gttccgcgaa tttgctatta cgctggcggt agcgattttg
    18481 atctcagcgg tggtgtcgct gaccctgaca ccgatgatgt gcgcgcggat gctcagccag
    18541 gagtcgttgc gtaaacagaa ccgcttctcc cgtgcctcgg aaaaaatgtt cgacaggata
    18601 atcgcctcct atggtcgtgg actggcgaag gtgctgaatc atccgtggct gaccttaagc
    18661 gtggcgctca gcacgctgct gcttagcgtt ctcttatggg tgttcattcc gaaaggtttc
    18721 ttcccggtac aggacaacgg cattattcag ggcactttgc aggcaccgca atccagctcc
    18781 tttgccaata tggcccagcg acaacgccag gtcgcggacg tgattttgca ggatccggca
    18841 gtgcaaagcc tgacctcatt tgttggcgtt gatggcacta acccgtcgct gaatagtgca
    18901 cgtttacaaa tcaacctcaa accgctggat gaacgtgatg accgagtgca aaaagtcatt
    18961 gcccgtctgc aaacggcggt ggataaagtg ccgggcgtcg atctcttcct gcaaccaacg
    19021 caggatctga ctattgatac tcaggtcagc cgcacccagt accagtttac cttgcaggcc
    19081 acctcactgg atgcgctcag tacctgggtg ccacagttga tggaaaaact ccagcaactg
    19141 ccacagcttt ctgatgtctc cagcgactgg caggacaaag ggctggtggc gtatgtcaat
    19201 gttgatcgcg acagcgccag ccgtctgggg atcagcatgg cggatgtcga taacgccctg
    19261 tacaacgcgt ttggtcagcg actgatttcc actatttata ctcaggctaa ccagtatcgc
    19321 gtagtgctgg agcacaacac cgaaattacc ccgggcctcg cggcgctgga taccattcgc
    19381 ctgaccagta gcgacggcgg cgtggtgccg ttaagctcaa ttgccaaagt tgaacagcgt
    19441 tttgcgccgc tttccatcaa ccatctggat cagttcccgg taacgaccat ctcctttaac
    19501 gtgccggata actattcgct gggcgatgcg gtgcaggcga ttatggacac cgaaaagacg
    19561 ctgaatctgc cggtggatat caccacgcaa tttcagggca gcaccctcgc cttccagtcg
    19621 gcgctgggca gcactgtctg gctgattgtc gcggcggtgg tggcgatgta tatcgtgctc
    19681 ggcattctgt acgagagctt tattcacccg atcaccattc tctcgacgct acccaccgca
    19741 ggggtcggcg cgctgctggc attaatgatt gccggtagcg aactggatgt gattgcgatt
    19801 atcggcatta ttttgctgat cggtattgtg aagaagaacg ccatcatgat gatcgacttc
    19861 gcgctggctg ctgagcgcga gcaaggcatg tcgccgcgcg atgcaatcta ccaggcttgt
    19921 ctgttgcgtt ttcgtccgat cctgatgacc accctggcgg ctctgcttgg cgcgctgccg
    19981 ctgatgttga gtaccggggt cggcgcggaa ctgcgtcgtc cgttaggtat cggcatggtc
    20041 ggcggtctga ttgtcagcca ggtgctgacg ctgttcacca cgccggtgat ttatctgctg
    20101 ttcgaccgcc tggcattgtg gaccaaaagt cgctttgctc gccatgaaga ggaggcgtaa
    20161 gtgaagtttt ttgccctctt catttaccgc ccggtggcga cgattttact gtcggtcgcc
    20221 attaccctgt gcggcatact gggcttccgt atgctgccgg tcgccccgct gccgcaggtc
    20281 gattttccgg tgattatggt cagcgcctcg ctgcccggtg cgtcaccaga aacaatggcg
    20341 tcttccgttg ccacgccgct ggagcgctca cttgggcgca ttgccggagt cagtgaaatg
    20401 acctccagca gttcgctcgg cagcacgcgt attattttgc agtttgattt tgaccgggat
    20461 atcaacggcg cggcgcgcga tgtgcaggcg gcgatcaacg ctgcacaaag tttgctgccc
    20521 agcgggatgc ccagccgtcc gacctatcgc aaagctaacc cgtcggatgc gccaattatg
    20581 atcctcacgc tgacatccga tacttattcg cagggtgaac tgtacgattt tgcctcgacg
    20641 cagctggctc cgacgatttc gcaaatcgac ggtgtcggtg atgtcgatgt tggcggcagc
    20701 tcgctgcccg ccgtacgcgt cgggctgaat ccgcaggcgc tgtttaatca gggcgtctcg
    20761 ctggacgacg tacgcaccgc catcagcaat gccaacgtgc gtaaaccgca gggggcgctg
    20821 gaagatggca ctcaccgctg gcagatccag accaatgatg agctaaaaac cgccgccgaa
    20881 tatcagccgt tgattattca ttacaacaac ggcggcgcgg ttcgtctggg cgatgtggcg
    20941 acggtgaccg actcggtgca ggatgtgcgc aacgccggga tgaccaacgc caaaccggct
    21001 attttgctga tgatccgcaa gctgccggaa gccaatatta tccagacggt agacagcatc
    21061 cgggcaaaat taccggagtt gcaggaaacc attccggcgg cgattgatct gcaaattgct
    21121 caggatcgct cccccaccat tcgcgcctcg ctggaagaag tcgagcaaac gctgattatc
    21181 tcggtggcgc tggtgattct ggtggtgttt ttattcctgc gctcgggtcg cgccactatt
    21241 attcccgccg ttgcggtgcc ggtttcgctg attggtacgt ttgcggcgat gtacctgtgc
    21301 ggattcagtc tcaataacct ttcgttaatg gcgctcacca tcgctactgg tttcgtggtg
    21361 gatgacgcca tcgtggtgct ggaaaacatt gcccgtcatc tggaagcggg gatgaaaccg
    21421 ctacaggccg cgctacaagg tactcgcgaa gtcggcttta cggtgctatc gatgagtctg
    21481 tcactggtgg cggtgttcct gccgctgctg ttgatgggcg gattgccggg ccgactgtta
    21541 cgcgaatttg ccgtgacgct ttctgtcgcc attgttatat cgttgctggt ttctctgaca
    21601 ttaacgccaa tgatgtgtgg ctggatgctg aaagccagca agccgcgtga gcaaaagcga
    21661 ctgcgtggtt ttggccgcat gttggtagcc ctgcaacaag gctacggcaa gtcgctgaaa
    21721 tgggtgctca atcatacccg tctggtgggt gtggtgctgc ttggcaccat tgcgctgaat
    21781 atctggctgt atatctcgat cccgaaaacc ttcttcccgg agcaggacac cggcgtgctg
    21841 atgggcggga tacaggcgga tcagagcatt tcgtttcagg cgatgcgcgg caaattgcag
    21901 gatttcatga aaattatccg tgacgatccg gcagtggata atgtcaccgg ctttaccggc
    21961 ggttcacgag ttaacagtgg gatgatgttt atcaccctca agccacgcga cgaacgcagc
    22021 gaaacggcgc agcaaattat cgaccgtctg cgcgtaaaac tggcgaaaga accgggggcg
    22081 aatctgttcc tgatggcggt acaggatatt cgcgttggtg ggcgtcagtc gaacgccagc
    22141 taccagtaca cgttgttatc cgacgacctg gcggcactgc gcgaatggga gccgaaaatc
    22201 cgcaaaaaac tggcgacgtt gccggaactg gcggacgtga actccgatca gcaggataac
    22261 ggcgcggaga tgaatctggt ttacgaccgt gacaccatgg cacggctggg aatcgacgta
    22321 caagccgcca acagtctgtt aaataacgcc ttcggtcagc ggcaaatctc gaccatttac
    22381 cagccgatga accagtataa agtggtgatg gaagtggatc cgcgctatac ccaggacatc
    22441 agtgcgctgg aaaaaatgtt cgttatcaat aacgaaggca aagcgatccc gctgtcgtat
    22501 ttcgctaaat ggcaaccggc gaatgcccca ctatcggtga atcatcaggg attatcggcg
    22561 gcctcgacca tttcgttcaa cctgccgacc ggaaaatcgc tctcggacgc cagtgcggcg
    22621 atcgatcgcg caatgaccca gcttggtgtg ccttcgacgg tgcgcggcag ttttgccgga
    22681 acagcgcagg tgttccagga gacgatgaac tcacaggtga tcctgattat cgccgccatc
    22741 gccacggtgt atatcgtgct gggtatcctt tacgagagtt atgtgcaccc gctgacgatt
    22801 ctctccaccc tgccctcggc gggtgttgga gcgctgctgg cgctggagct gttcaatgcc
    22861 ccgttcagcc taatcgccct gatagggatc atgctattaa tcggcatcgt gaagaaaaac
    22921 gccattatga tggtcgattt tgcgcttgaa gcccaacggc acggtaacct gacgccgcag
    22981 gaagctattt tccaggcctg tctgctgcgt tttcgcccga ttatgatgac taccctggcg
    23041 gcgctgtttg gcgcgctgcc gctggtgttg tcgggcggcg acggctcgga gctgcggcaa
    23101 cccctgggga tcaccattgt cggcggactg gtaatgagcc agctccttac gctgtatacc
    23161 acgccggtgg tgtatctctt tttcgaccgt ctgcggctgc gtttttcgcg taaacctaaa
    23221 caaacggtaa ccgagtaaat gacagatctt cccgacagca cccgttggca attgtggatt
    23281 gtggctttcg gcttctttat gcagtcgctg gacaccacca tcgtaaacac cgcccttccc
    23341 tcaatggcgc aaagcctcgg ggaaagtccg ttgcatatgc acatggtcat tgtctcttat
    23401 gtgctgaccg tggcggtgat gctgcccgcc agcggctggc tggcggacaa agtcggcgtg
    23461 cgcaatattt tctttaccgc catcgtgctg tttactctcg gttcactgtt ttgcgcgctt
    23521 tccggcacgc tgaacgaact gttgctggca cgcgcgttac agggcgttgg cggcgcgatg
    23581 atggtgccgg tcggcagatt gacggtgatg aaaatcgtac cgcgcgagca atatatggcg
    23641 gcgatgacct ttgtcacgtt acccggtcag gtcggtccgc tgctcggtcc ggcgctcggc
    23701 ggtctgctgg tggagtacgc gtcgtggcac tggatctttt tgatcaacat tccggtgggg
    23761 attatcggtg cgatcgccac attgatgtta atgccgaact acaccatgca gacgcggcgc
    23821 tttgatcttt ccggcttttt attgctggcg gttggcatgg cggtattaac cctggcgctg
    23881 gacggcagta aaggtacagg tttatcgccg ctggcgattg ctggcctggt cgcagttggc
    23941 gtggtggcac tggtgcttta tctgctgcac gccagaaata acaaccgcgc cctgttcagt
    24001 ctgaaactgt tccgcactcg taccttttcg ctgggcctgg cggggagctt tgccggacgt
    24061 attggcagtg gcatgttgcc ctttatgaca ccggttttcc tgcaaattgg cctcggtttc
    24121 tcgccgtttc atgccggact gatgatgatc ccgatggtgc ttggcagcat gggaatgaag
    24181 cgaattgtgg ttcaggtggt gaatcgcttt ggttatcgtc gggtactggt agcgaccacg
    24241 ctgggtctgt cgctggtcac cctgttgttt atgactaccg ctctgttggg ctggtactac
    24301 gttttgccgt tcgtcctgtt tttacaagga atggtcaact caacgcgttt ctcctccatg
    24361 aacaccctga cgctgaaaga tctcccggac aatctggcga gcagcggaaa cagcctgctg
    24421 tcgatgatta tgcaattgtc gatgagtatc ggcgtcacca tcgccgggct gttgctggga
    24481 ctttttggtt cgcagcatgt cagcgtcgac agcggcacca cacaaaccgt ctttatgtac
    24541 acctggctta gcatggcgtt gatcatcgcc cttccggcgt tcatctttgc cagagtgccg
    24601 aacgatacgc atcaaaatgt agctatttcg cggcgaaaaa ggagcgcgca atgaagttct
    24661 ggcgacccgg tattaccggc aaactgtttc tggcgatttt cgccacctgc attgtcttgc
    24721 tgatcagtat gcactgggcg gtgcgtatca gttttgagcg tggctttatt gattacatca
    24781 agcatggtaa tgaacaacga ttacaactgt taagtgatgc gcttggcgag cagtatgcgc
    24841 agcatggcaa ctggcgcttc ctgcgcaaca atgatcgctt tgtctttcag atcctgcgtt
    24901 catttgaaca cgataattcg gaagataaac ccggcccggg tatgccaccg cacggctggc
    24961 gtacccagtt ctgggtggtt gatcaaaaca acaaagtgct ggttggtccg cgagcgccga
    25021 ttccacctga cggtacacgg cgacccattc tggtcaacgg tgcggaagtt ggcgcggtga
    25081 tcgcctcccc cgttgagcgg ttaacgcgca atactgatat caatttcgat aaacaacagc
    25141 ggcaaaccag ctggctgatc gtcgccctgg caacgttact cgcggcgctc gccacctttc
    25201 tgctggcgcg cggtttgctg gcaccggtaa aacgacttgt cgatggcacg cacaaactgg
    25261 cggcgggcga tttcactacc cgcgtaacgc ccaccagtga agatgaactg ggcaaactgg
    25321 cgcaagactt caaccagctt gccagcacgc tggagaaaaa ccagcaaatg cggcgcgatt
    25381 ttatggccga tatttcacac gaactgcgta cgccattagc ggtgctgcgc ggtgaactgg
    25441 aagccattca agatggcgtg cgcaaattca cgccggagac ggtggcgtct ttacaggcgg
    25501 aagtcggtac actgactaaa ctggttgacg atcttcatca gttgtcgatg tctgatgaag
    25561 gcgctctcgc ctatcaaaaa gcaccggtag atttgatccc actgctggaa gtggcgggcg
    25621 gcgcatttcg cgaacgattt gccagccgtg gcctgaaact gcagttttcc ctgccagaca
    25681 gtattaccgt atttggcgat cgcgaccgtt taatgcagtt attcaataac ttactcgaaa
    25741 acagcctgcg ctacactgat agcggcggca gcctgaaaat ctctgccgag cagcacgaca
    25801 aaacggtgcg cctgaccttt gccgacagtg cgccaggtgt cagtgacgag cagctacaaa
    25861 aattatttga acgtttttat cgcaccgaag gttcccgcaa ccgcgccagc ggtggttccg
    25921 ggctggggct ggcgatttgc ctgaacattg ttgaagcaca taatggccgc attattgctg
    25981 cccattcgcc ttttggcggg gtaagcatta cagtagagtt accgctggaa cgggatttac
    26041 agagagaagt atgaccgagt taccaatcga cgaaaacaca ccgcgtattt tgatcgtgga
    26101 agatgaaccg aagctggggc agttgctcat tgattatctg cgtgctgcga gctatgcgcc
    26161 gacgcttatc agccacggcg atcaggtact gccgtatgtg cgccagacac caccggatct
    26221 gatcctgtta gatctgatgc tccctggcac cgatggcctg acgctgtgcc gggaaattcg
    26281 tcgtttttct gacattccga tcgtgatggt gacggcaaaa atcgaagaga tcgatcgcct
    26341 gctggggctg gagattggcg cagatgatta tatctgtaag ccgtacagcc cacgggaagt
    26401 ggtagcgcgc gtcaaaacca ttttgcgccg ttgcaaaccg cagcgcgagt tgcagcaaca
    26461 ggatgctgaa agcccgttga ttatcgacga aggtcgtttt caggcttcat ggcgcggtaa
    26521 aatgcttgac ctgacgcctg cggaatttcg tctgctgaaa acgctctctc atgaaccagg
    26581 aaaagtgttc tcccgcgagc atttgctcaa tcatctttat gacgactacc gcgtagtaac
    26641 cgaccgcacc atcgacagcc acattaaaaa cctgcgccgc aagctggaat ctctcgacgc
    26701 cgaacagtca tttatccgcg ccgtttacgg cgttggttac cgctgggaag ccgacgcctg
    26761 ccgcatcgtt tagttttagc gacatgactt tgttagccgg agatggcgtt cagaccagat
    26821 ccggcaacat tatcccacgc atggtcagca aactgacatt atctctccgc cctggcaggc
    26881 atcagaaaat cgctcatact ttaatcggta aacagcacct ttagatgctg ttttcgatac
    26941 gcaatttcaa tcaaggagtc attatggctg gttggtttga actcagtaag agcagtgata
    27001 atcagttccg gtttgtgcta aaagcgggca atggtgagac tatcctcacc agcgagctgt
    27061 atacctcaaa agcctctgcg gaaaagggca tcgcgtcggt gcgtagcaac agcccgcaag
    27121 aagaacgcta tgagaaaaaa acggcaagta acggcaaatt ctatttcaat ctgaaagccg
    27181 ctaatcatca aattatcggc tccagccaga tgtacgccac cgcgcaatct cgtgaaaccg
    27241 gaattgcctc cgttaaagcc aatggcacaa gccagacggt gaaagacaat acgtaattac
    27301 gatgccgggc gtgggttgtt ggcgtccggc tttatatcca aataacgctt agttattaat
    27361 aattaagagt aaccgccgcc attaaaatag tgggcactga cgagcactcg ttgtgctttc
    27421 tttgtttacc atcccatact tatcctcctg aacatgttca tgtgccaaaa tatcttctaa
    27481 tactttcgtg acgctatccg gtttatgaca tacactgatg taagattttt acttacaggc
    27541 gggcgtaaaa tggccagaac gatgacggtt gatctcggcg atgagttgcg tgaatttatc
    27601 gagtccttaa ttgaatccgg cgactatcgc acccaaagcg aagtgatccg tgagtcactg
    27661 cgattacttc gagaaaagca agcagagtct cgcctgcaag cgttacgtga catgctcgca
    27721 gaagggttga gtagtggtga agctcagccg tgggaaaagg acgcgttctt acggaaggtg
    27781 aaagcaggga taaggaaata atgtgcgtat tattaaactc atgccaaaag ccaatgagga
    27841 tttagaaggc atctggtatt acagctatca ccatttcggc gaaccacaag ccgacagata
    27901 tgtggaacat ctttcagacg ttctccagat tctgagtaac aataatatag gtacgcccag
    27961 acctgaatta ggcgaaggta tttttgttct tccttttgaa cgccacgtca tttatttcct
    28021 gcaatcacca ggggaaatta tcgtcattcg tatccttaac caaaaccagg acgccaccag
    28081 acatttacac tggagctaaa cccgccgatt gcgctacaat gcccgccctt aaagtggggg
    28141 cactccccta accgcttcat caggtgaagc ggatctgacc tgtcatcaga acgagagaat
    28201 tatgtttaaa ccggaactcc tttccccggc gggaacgctg aaaaatatgc gttacgcttt
    28261 cgcttatggc gcagatgctg tttatgcggg ccagccgcgt tattccctgc gtgtgcgcaa
    28321 caacgaattc aaccacgaaa atcttcagct cggcatcaat gaagcccacg cgctggggaa
    28381 aaagttttat gtcgtggtca acattgcacc gcacaacgcc aagctgaaaa cctttatccg
    28441 tgacctgaaa ccggtggtgg aaatggggcc ggatgcgctg attatgtccg atccagggct
    28501 gattatgctg gtgcgtgagc acttccctga aatgccgatc cacctttcgg tgcaggctaa
    28561 cgccgtgaac tgggcgacgg tgaaattctg gcagcaaatg ggcctgaccc gcgtgatcct
    28621 ctctcgcgag ctgtcgctgg aagagattga agagatccgc aatcaggtgc cggatatgga
    28681 gatcgagatc ttcgttcacg gcgcgctgtg catggcctac tccggtcgct gcctgctctc
    28741 tggctatatc aacaagcgcg acccgaacca gggcacctgc accaacgcct gccgctggga
    28801 gtacaacgtc caggaaggga aagaagatga tgttggcaac atcgtacaca agtacgagcc
    28861 gattccggtg caaaatgttg agccgacgct gggtatcggc gcaccaaccg acaaagtgtt
    28921 tatgatcgaa gaggcccagc gtccgggcga gtatatgacc gcgtttgaag atgagcacgg
    28981 cacttacatc atgaactcga aagatctgcg cgccatcgcc catgtagaac gcctgaccaa
    29041 aatgggcgtg cattcgctga aaatcgaagg tcgtaccaaa tctttctact attgtgcacg
    29101 caccgcacag gtttaccgca aagctatcga tgacgccgct gcgggaaaac cgttcgatac
    29161 cagcctgctg gaaactctgg aaggtctggc gcatcgtggc tataccgaag gtttcctgcg
    29221 tcgtcatact cacgacgatt atcagaacta cgaatacggt tattcagttt ctgaccgcca
    29281 gcagtttgtt ggtgagttta ccggtgagcg caagggggac ctcgcggcgg tagcggtgaa
    29341 aaataaattc tccgttggcg acagccttga gctgatgacg ccgcaaggca acattaattt
    29401 tacccttgag cacatggaaa acgccaaagg cgaagctatg ccgatagcac caggcgatgg
    29461 ttatactgtg tggctcccgg tcccgcagga tcttgagctc aattacgcgc tgctgatgcg
    29521 taatttctcc ggggaaacca cgcgtaatcc ccacggtaag tgattaattt cgattatttt
    29581 tcccggatgg aaaattctta gaaaccgatc acatacagct gcatttatta aggttatcat
    29641 ccgtttcgct gaaaaacata acccataaaa tgctagctgt accaggaacc acctccttag
    29701 cctgtgtaat ctcccttaca cgggcttatt ttttatgcat aagccctatc cctggtcacc
    29761 gtcttccatt gaccacatcg atagaatctc ccttcatagc acgatgcctt tcacgtaacg
    29821 gcatcgtgct cgcacaggtt ccggctaagc acaaccagaa cgcgcatgtt tgacgcttac
    29881 caaaaaatat tctcactctc cacatttgaa tgtcagacga gcgacgccat gtaatcctgc
    29941 accttctgtc ttcaggtcaa ctatctgcat ttttttgccc tgagtaacac agaaatgggc
    30001 tgcatcattt tttactatat tttctgcacc agatattctg cccctggcta aagaagcttc
    30061 ggcttcggtg tagtattggt tatcgagttt acgctgaata ttacttttat atgcaagacc
    30121 aaatttaccg atacttgtct catcattatg cacagcacaa ccagacataa taaaaatact
    30181 aattaatgat atagcagcta tctttttcat ctcaccttcc cccattaaat accaacgaca
    30241 ctctctagtg tttaaatata ataatggcat gattattata attgaatagg attataataa
    30301 atgttctgta caacatttcc ta
//
LOCUS       DAZGLJ010000031        30295 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000031 DAZGLJ010000000
VERSION     DAZGLJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.031"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHK49_001309"
     CDS             complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHK49_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO9261983.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHK49_001310"
     CDS             complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHK49_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO9261984.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHK49_001311"
     CDS             complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHK49_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO9261985.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLSVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(1087..1581)
                     /locus_tag="ACHK49_001312"
     CDS             complement(1087..1581)
                     /locus_tag="ACHK49_001312"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO9261986.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHK49_001313"
     CDS             complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHK49_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO9261987.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHK49_001314"
     CDS             complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHK49_001314"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO9261988.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHK49_001315"
     CDS             complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHK49_001315"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO9261989.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHK49_001316"
     CDS             complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHK49_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO9261990.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHK49_001317"
     CDS             complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHK49_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO9261991.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHK49_001318"
     CDS             complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHK49_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO9261992.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            8195..9235
                     /gene="gpr"
                     /locus_tag="ACHK49_001319"
     CDS             8195..9235
                     /gene="gpr"
                     /locus_tag="ACHK49_001319"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO9261993.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(9275..9769)
                     /locus_tag="ACHK49_001320"
     CDS             complement(9275..9769)
                     /locus_tag="ACHK49_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO9261994.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIINVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9960..10844
                     /gene="yghA"
                     /locus_tag="ACHK49_001321"
     CDS             9960..10844
                     /gene="yghA"
                     /locus_tag="ACHK49_001321"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO9261995.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHK49_001322"
     CDS             complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHK49_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO9261996.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHK49_001323"
     CDS             complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHK49_001323"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO9261997.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            12534..13721
                     /gene="metC"
                     /locus_tag="ACHK49_001324"
     CDS             12534..13721
                     /gene="metC"
                     /locus_tag="ACHK49_001324"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO9261998.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13861..14520
                     /gene="yghB"
                     /locus_tag="ACHK49_001325"
     CDS             13861..14520
                     /gene="yghB"
                     /locus_tag="ACHK49_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO9261999.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHK49_001326"
     CDS             complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHK49_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO9262000.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHK49_001327"
     CDS             15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHK49_001327"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO9262001.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHK49_001328"
     CDS             16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHK49_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO9262002.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17948..18874
                     /locus_tag="ACHK49_001329"
     CDS             17948..18874
                     /locus_tag="ACHK49_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO9262003.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18925..19182
                     /gene="yqhH"
                     /locus_tag="ACHK49_001330"
     CDS             18925..19182
                     /gene="yqhH"
                     /locus_tag="ACHK49_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO9262004.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(19225..21444)
                     /locus_tag="ACHK49_001331"
     CDS             complement(19225..21444)
                     /locus_tag="ACHK49_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO9262005.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="ACHK49_001332"
     CDS             complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="ACHK49_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO9262006.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="ACHK49_001333"
                     /gene_synonym="parF"
     CDS             complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="ACHK49_001333"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO9262007.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(24012..26270)
                     /gene="parC"
                     /locus_tag="ACHK49_001334"
     CDS             complement(24012..26270)
                     /gene="parC"
                     /locus_tag="ACHK49_001334"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO9262008.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLEQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(26816..27298)
                     /locus_tag="ACHK49_001335"
     CDS             complement(26816..27298)
                     /locus_tag="ACHK49_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO9262009.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPIMFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="ACHK49_001336"
     CDS             complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="ACHK49_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO9262010.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            27895..28554
                     /gene="qseB"
                     /locus_tag="ACHK49_001337"
     CDS             27895..28554
                     /gene="qseB"
                     /locus_tag="ACHK49_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO9262011.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            28551..29900
                     /gene="qseC"
                     /locus_tag="ACHK49_001338"
     CDS             28551..29900
                     /gene="qseC"
                     /locus_tag="ACHK49_001338"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO9262012.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(29946..30278)
                     /locus_tag="ACHK49_001339"
     CDS             complement(29946..30278)
                     /locus_tag="ACHK49_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO9262013.1"
                     /translation="MLKHKIQTIFEALLYIMLTYWLIDAFFAFNKYDWMLESGDNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLF
                     WGRFIFCY"
BASE COUNT         7487 a         8087 c         7641 g         7080 t
ORIGIN      
        1 aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg
       61 ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact
      121 ggcccagtag atcggcaggg ttaccttcac aaatcagggc gatattcacg tcgcgcttga
      181 taccacatac ttcgacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc
      241 ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt ttgacgatca
      301 gcgaatcgcc ggtatcgaca cgcaaccgct cgccgtgaca gagcggacac tgcgcatcgt
      361 gctgatgaat ctccaccacc tggctgcaat cccagcacca agcctgggcg ggtttataga
      421 cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac
      481 ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc
      541 gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagag
      601 acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt
      661 ggtacatccg gattcgtgac tgacagcgaa aggatcattc gtgcgcagtc atccgtcagg
      721 cgttggccct cttcaatcga catgctgtgc gaaagcggcg acatcagcga acaggagaga
      781 tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tagtgccata cggcagttgc
      841 agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata
      901 actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta
      961 aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc
     1021 cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct
     1081 atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgccagatt cacgcagcgc
     1141 agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat
     1201 gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcacc
     1261 ggtgaagcgc agggccgaca atacgtcggc caggccaagc tgatgcggag agattttgtt
     1321 ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg gcgtgttctt
     1381 tttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag
     1441 ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg
     1501 ctctaacgct tcgacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgac
     1561 ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcggca
     1621 tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact
     1681 tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc gtaaggaccg
     1741 acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac tgcctggtag
     1801 ttgctgatga taccgtcttt aatcaccatc cagtgagaga gcataccgcg cggcgcttcg
     1861 aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac aaaggtggtg
     1921 tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc
     1981 aattcacagc agtgaacggt acgaccaata atacggccca gcgtggagtg cagctgtgcc
     2041 acttccagcg tgttgccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc
     2101 tgggtagatt ctcgacctgc cgccagtttc accagcatat tagccagcgg acccacttct
     2161 accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa
     2221 ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc gtctttatac
     2281 caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg
     2341 atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg
     2401 ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg
     2461 cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt ataaacctgc
     2521 tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc
     2581 aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccactgc caggttctga
     2641 atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc
     2701 agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgct
     2761 gggtgacccc agtagccatt agcgaaaata cccaactgac cgctggcaac cagatctttg
     2821 atcttgttct gaactttagt gaactcttcc ggactgttca gatgccaggt cgaaacgcct
     2881 ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat gtccacccag
     2941 tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga
     3001 atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc
     3061 gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc
     3121 atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta
     3181 ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc
     3241 ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat
     3301 gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga
     3361 tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg
     3421 ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg
     3481 ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa tcattacgca
     3541 gcttcgccac acgcagaacc accagcggga agagcatcag caggacttca atccagaaca
     3601 tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc
     3661 caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttgacga
     3721 acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc gaaccttcaa
     3781 agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt
     3841 catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagt gaccccattg
     3901 aagactggtg catggtcggc aacagcgcgc caagtgcaat gatgaagaac atcaccttgt
     3961 tcagacgctt gagagaaacc ttccagccca gacgttcaaa cagtgccgga gcaaactcca
     4021 gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt accgagttca
     4081 cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag
     4141 tgatcgacaa gccccccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca
     4201 gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac
     4261 aggcaaagcc ggtaccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc
     4321 cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac
     4381 agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg
     4441 gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga
     4501 cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg
     4561 gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg
     4621 agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg
     4681 ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc
     4741 gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc
     4801 gccatcagct cttcacgcgt accaaaaatt accgcgcccg ccgggcacac ttctacgcag
     4861 ccaggcaaac cgcctttatc gagacgttcc acacctttct ggttgcacaa ctcgcactta
     4921 tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtatgg gcaggcgacc
     4981 atgcagtaac ggcaaccggt gcacacatct ttgtcgtaat ggacaatgcc ggttttcgga
     5041 tcttttttca gcgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata
     5101 cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgccg
     5161 ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag
     5221 gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa
     5281 gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc
     5341 gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg
     5401 caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt
     5461 tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca
     5521 gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag
     5581 cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga
     5641 tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga ccaatcgcta
     5701 ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg
     5761 gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca tcaccgaact
     5821 ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac
     5881 ggccataggc gaaggtcgga cgatttttgt catccagttt cggcggtttg ccgtaagtga
     5941 tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga
     6001 taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag
     6061 ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct gctgcgcctt
     6121 ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa taaataccgt
     6181 tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg tacttctcaa
     6241 gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat
     6301 actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt gcacgaagca
     6361 gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg
     6421 ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg
     6481 ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg
     6541 tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca
     6601 atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga
     6661 tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta
     6721 aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg
     6781 caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc
     6841 atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc
     6901 gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt
     6961 cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa
     7021 atggttattc atcgttcacc tcattcgcta tatatcgcca tatatagcga tattttaggc
     7081 aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa tccagtaaga
     7141 gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat
     7201 cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg
     7261 gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg taagcaggcc
     7321 agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct
     7381 tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg
     7441 gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca
     7501 ctttgcctgc cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca
     7561 tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat
     7621 aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc
     7681 gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc actaccacgg
     7741 ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac
     7801 catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct
     7861 gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc
     7921 ccaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc ccgtgagcgt
     7981 aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg
     8041 ttgcactcct caactggtgt ttttttaagc aaagcataag cacgtgtttt tgcccagttt
     8101 ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca
     8161 ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg
     8221 ttacgggcag atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc ccgcgttatc
     8281 gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct
     8341 gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt acgggccgcc
     8401 tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg ccgcttatcg
     8461 cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc cttacggctc
     8521 tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct
     8581 tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac
     8641 cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc
     8701 ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct
     8761 tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa gcggcctgct
     8821 ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg ctcagggatt
     8881 gctgaccgga aaatatctca acggcattcc gcaagattca cggatgtatc gtgaagggaa
     8941 taaagttcgt ggtctgacac cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt
     9001 attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt taagctggtt
     9061 gttgaaagat gatcgcgtga cgtcggtatt gattggtgcc agccgcgcgg agcaactaga
     9121 ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg cgcagattga
     9181 tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata aatgacctgt
     9241 taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt
     9301 caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg acgtaccaca
     9361 tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtagg tgaatggaag
     9421 aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca
     9481 gccagttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa ttctcataac
     9541 cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca
     9601 acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg ttaataatct
     9661 cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag tacacggggg
     9721 caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc
     9781 gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca acagcaaatc
     9841 aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc ctggttccct
     9901 tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg agaaaaacga
     9961 tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac
    10021 aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc gagaaaacct
    10081 atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc gattccggaa
    10141 taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc
    10201 ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggacgca
    10261 aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg ctggttcacg
    10321 aagcgcacaa ggcgttaggt gggctggata ttatggcgct ggtcgccggg aaacaggttg
    10381 ccattccgga tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg
    10441 ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa ggtgcaagta
    10501 tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg
    10561 ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc gcggagaaag
    10621 gtattcgggt gaatattgtc gcgccaggcc ctatctggac ggcactgcaa atttccggcg
    10681 gacaaacgca ggataagatc ccgcagtttg gtcagaaaac gccgatgaag cgtgcggggc
    10741 aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca
    10801 ccgcagaagt gcacggtgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt
    10861 gaaaagcaac cgggcatcat tgtgaattca ctcttacctg atgtggcgaa aacgccttat
    10921 ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc ctgataagcg
    10981 tagcgcatca ggcattgagc acctaatgcc ggatgcggcg tgaacgcctt atccggccta
    11041 caaacgcacg aagattcaac atgttgcaat agatattgta ggcctgatga gcgtagcgca
    11101 tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac ctatcttcag
    11161 gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat cgacggtttt
    11221 atccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt
    11281 aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt ctgccttcac
    11341 cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag caggcaagtt
    11401 caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca gcagaaccaa
    11461 catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt
    11521 ttcgttaaga tgcattgcca tcggatatta tcctgcgcgt aatttttgtg cgacacgaac
    11581 cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca acaatacctg
    11641 cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa atacgttata
    11701 gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca gagcttctgc
    11761 gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac caataaagct
    11821 gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg cgccgatggt
    11881 tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc gacgctccag
    11941 acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg acagttccag
    12001 ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa aatcagcggc
    12061 gatatcgttg gcctggttta aggaacgcgc ttcagccagc agttgctgct cgcgcttaag
    12121 gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc aagtgactac
    12181 ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt gctgatacat
    12241 accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc tccaggacgc
    12301 aagtcacaaa atctgcgcat aataatatca aaatgacgtc gaattgatag tcgttctcat
    12361 tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc agtaaaaaag
    12421 tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag gatgcgcccg
    12481 cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc gacatggcgg
    12541 acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac actctcggcg
    12601 cggtaaatag cgtgattcag cgcgcttctt cgctggtctt tgacagtgtg gaagccaaaa
    12661 agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc ggaacgttaa
    12721 cccatttctc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc tgcgcgctat
    12781 ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc ttttgtcgaa cagggcgatc
    12841 atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc aaaatcctca
    12901 gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat atcgttaagc
    12961 atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc aggctccatc accatggaag
    13021 tccacgacgt tccggcgatt gttgccgccg tacgcagtgt ggtgccggat gccatcatta
    13081 tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg
    13141 tttctattca agccgccacc aaatatctgg ttgggcattc agatgcgatg attggcactg
    13201 ccgtgtgcaa tgcccgttgc tgggagcagc tacgggaaaa cgcctatctg atgggccaga
    13261 tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta ggtgtgcgtt
    13321 tgcgtcaaca tcatgaaagc agtctgaaag tggctgaatg gctggcagaa catccgcaag
    13381 ttgcgcgagt taaccaccct gctctgcctg gcagtaaagg ccacgaattc tggaaacgag
    13441 actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc aataatgaag
    13501 agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg tggggcgggt
    13561 atgaatcgtt gatcctggcg aatcaaccag aacatatcgc cgccattcgc ccccaaggcg
    13621 agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat gtcgacgatc
    13681 tgattgccga tctggacgcc ggttttgcgc gaattgtata acattgccac ttttggacaa
    13741 ttttgcagac atttaattgt gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc
    13801 ggacgaatca ggagtacaat aggcagataa aggcttaaac gctgttccac aggaaagtcc
    13861 atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc cgcgctggcg
    13921 gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac
    13981 ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc aggcgcattg
    14041 attgcccaag gggttatgga ttttctgcct acgattgcga ttctgaccgc cgcagcaagt
    14101 ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa aacggtgaaa
    14161 ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat gtttgaccgc
    14221 cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac gctgctgcca
    14281 accatggcgg gaatttccgg tctgccaaac cgccgcttcc agtttttcaa ctggttaagt
    14341 ggattgctgt gggtcagcgt ggtaaccagt tttggctatg ccttaagtat gattccgttc
    14401 gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat tgccttgtta
    14461 accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg taacgcctga
    14521 cgattttccc cgttcccggc tgctgtaccg ggaacgtatt taattcccct gcatcgcccg
    14581 cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact cacggctaaa
    14641 ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctggccttca tgccgtcatg
    14701 gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca acggcgaggt
    14761 gctggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg cttctgccgc
    14821 cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca gcacgcggct
    14881 aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc
    14941 ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac gcgcatccag
    15001 tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt
    15061 ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt cgccaatgtc
    15121 catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg ccagcggcac
    15181 ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca gcaggtattc
    15241 attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc cggaaaagag
    15301 aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa
    15361 caaacgtaca tcgggcaaca gttctgacaa actattttct ttaattttca gtttattaac
    15421 tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt tcttaggaat
    15481 aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct ggaggaatag
    15541 gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac aatgaaaaac
    15601 atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag taatgaacaa
    15661 ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg
    15721 cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa
    15781 aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc tggaatttgg
    15841 cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac tggttcgcga
    15901 acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg gcaccaaatt
    15961 tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc tgcaaacggg
    16021 cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg
    16081 ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt
    16141 ccattctgcc catgttcagc cggtatttgc cgtgctcgat ccggtttata cctacaccct
    16201 gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg tggaacagta
    16261 tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca ttttgctgac
    16321 gctgatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg tgcgcgccaa
    16381 cgtcatgtgg gcggcgacgc aggcgctgaa cggtttgatt ggcgctggcg taccgcagga
    16441 ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg atcacgcgca
    16501 aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca agcgcgctaa
    16561 gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgatg atgagcgtat
    16621 tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct
    16681 ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac tggaagagca
    16741 cggcatgacc caactgggcg aaaatcatga cattacgttg gatgtcagcc gccgtatata
    16801 cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat ttcgtccaga
    16861 cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa ggaggaacgt
    16921 atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca gctgggactg
    16981 ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg
    17041 ggttatcgct cgattgatac cgccgcggcc tacaagaacg aagaaggtgt cggcaaagcc
    17101 ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac
    17161 gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca gcttgattat
    17221 atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg
    17281 aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc
    17341 cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag
    17401 atcgaacttc atccgctgat gcaacaacgc cagctacacg cctggaacgc gacacacaaa
    17461 atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa
    17521 gtcattcgcg atctggcaga taaatacggc aaaaccccgg cgcagattgt tatccgctgg
    17581 catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg tattgccgaa
    17641 aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc
    17701 gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaaca tgcaaattct
    17761 cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc ccctcgcgtc
    17821 ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc atcatgcaga
    17881 gccggaagct cttaaaagaa caactcatct atatccggga taaacgcaac ggagaggtga
    17941 aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta ccgtacacgc
    18001 acagcccccc tcactgaccg tagaacaaac agtccggcat atttatcaga actataaatc
    18061 agatgccact gccccttatt ttggtgaaac cggagagcgg gcgataactt ctgcgcgtat
    18121 tcaacaggcg cttaccctga acgacaatct tacgctgccg ggcaatattg gctggctgga
    18181 ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgctagaaa gcgttgcgat
    18241 aacccaaact gacgccgatc atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga
    18301 taaagaaaag accacgcaga cactgaaaat ggtggcggaa aatggtcgtt gggtcattga
    18361 cgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac
    18421 gctggccgct ttagcttcgt tgcaaaaaga acagccggaa gcctttgttg ccgaactctt
    18481 tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact cttaccgcca
    18541 ggcggttaat gccttctata aaaccacctt caagacggcc aataatcccg atgaagatat
    18601 gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag agtcgctatt
    18661 ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc gtattcatgt
    18721 tcgttttacg ctgaccaatg gcaacaacga agagcaagaa ctggttttac agcggcgcga
    18781 aggcaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac ttaagcagat
    18841 tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaataaca attagccgga
    18901 acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagc tgttgttctg
    18961 gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcagct ggcgagcaat
    19021 gtgcaaacat taaatgccaa aatcgcccgg cttgagcagg atatgaaagc actacgccca
    19081 caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga tgctcaggac
    19141 tattttgatt gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa tccccggcag
    19201 catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc gtttcttcgc
    19261 accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag caggcgtctg
    19321 acgctgggtc gccatcggcg tatgtttcgt caacgccgga cgggtattgc ggttctggcg
    19381 acgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc gacggctgcc
    19441 aatcagatgc tttttgccca tcgcttccag cgcctggcgg attaacggcc agtttgccgg
    19501 atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct tcggtacgaa
    19561 gacgtcttcg ctcttataac caatcttcgc cagcgggttt ttcccggtgt aatacatggt
    19621 ggttgagttc gccagcggcg atgggtagaa gttctgtacc tggtcgagac ggaaacgatg
    19681 ctttttcagc cacagcgcca gattcaccat atcttcatca cgcgtaccgg ggtgcgcgga
    19741 gatgaaatag gggatcagat actgctcttt acctgcctgt tttgagtaag tatcgaacag
    19801 ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgata acggcccttc
    19861 ttcggtatgt tccggggcaa tcttcagata accgccgacg tgatgggtcg ccaactcttt
    19921 gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcga tgaggatttt
    19981 tttaatgcct ttcagatcac gcgcacggcg atagaggttg atcgtcggtt cgtggttagt
    20041 gtccatgtgc ggacaaatat ccggataaac gcacgacaaa cggcgacaag tttgttcagc
    20101 gcgtggcgat ttgcagcgca acatatacat gttggcagtt ggcccaccga gatcggaaat
    20161 tacgcccgta aaaccaggaa cggtgtcgcg gatcgcttcg atctcattaa tgatcgaatc
    20221 ttccgaacgg ctctgaataa tgcgcccttc gtgctcggtg atagaacaga aagagcagcc
    20281 gccaaagcag ccgcgcataa tgttgaccga gaaacggatc atttcgtaag ccggaatacg
    20341 ggcattgcca taggccggat gcggcacgcg cttgtacggc agcgcaaaaa cgctgtccat
    20401 ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt caccgtgttt
    20461 ttgcatcaat gcgcgggcgc agcctgggtt ggtttcgtgg tgcagaatgc gcgaagcatg
    20521 agcgtacagg actttatcgc ccttcacttt ctcgaaagaa ggcagcaata cgtaggtttt
    20581 ttcccacggt ttcgggcgcg gcggctgcac ggttacagct ttcgcttcct gctttttcgg
    20641 cgcaacaggt ttgttatccg cgcacggcag atcttcgcca tacggatgcg ggattgggtc
    20701 gattttccct ggggtatcaa gacgagtaga atccacaccg ctccagccag gcagcgcctc
    20761 ttttacgata attgcggtat tacgcacatc gcggatttcg ctaattggct cgcccatcgc
    20821 cagacgatgc gctacttcca ccagcggacg ttcaccgtta ccaaacatca gcatgtcggc
    20881 tttcgaatcc accagcacgg aacggcgcac ggtatcggac cagtaatcat aatgcgcggt
    20941 acggcgcaga ctagcctcaa taccgccaag gatcaccggc acatctttcc acgcctcttt
    21001 acaacgctgg gtataaacca gtgtggcgcg atccggtcgc ttacccgcga cgttatccgg
    21061 tgtgtaggca tcgtcatgac gtaaacggcg atcggcggta tagcggttga tcatcgaatc
    21121 catgttgcca gcagtaacac caaaaaacag attcggttta cccagacgca taaagtcgtc
    21181 tttgctgctc cagtccggct gggcgatgat cccgacgcga aagccctgcg cttccagcat
    21241 acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc cagtaaccaa
    21301 aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca tcggcaaaaa
    21361 cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt cgcgatccgg
    21421 ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat aatcaaaggc
    21481 cggggattat aagccggaac gaaagagaaa tcgaaaggta ttccatactc gccctcctcg
    21541 ggcgagtatg aagattacgg taccggattg accaacagtt gcccaatcga gccacggtcc
    21601 gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga aggctgaccg
    21661 aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca gcctctgtct
    21721 tccgggaacg gcatcgcgcc attcacgtta cggatctgga acatcacgcc ttcaatatgg
    21781 aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg ctgcgcggtg
    21841 acatcaatac ggttgacgtc ccacagctgt ccattaatac ccgggtcatc acccagacta
    21901 atatcgcgac tgcgaatcgg cgaaccggcc atgatttcag ttggcagcaa gcgcatcgga
    21961 agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac cagggtagaa
    22021 accaaaatgc tggatggctc aaagaagcca cgaatacgat caacaatgct cgccgcttcg
    22081 ccacaggtga tcgacacttc atcaccgttg ctcatatcca ccagaatctc gcggcgctcg
    22141 cccggtgcca gcgaaagttg cttcaccgat acaggagcag gcaggaatcc ctgatcgcca
    22201 gaaatcacat gtaacgggcg accatcgctc atctgtagtt gatagcgacg agagttcgac
    22261 gcgttcagca gtcgcaagcg cacccagcca cgcgagactt caacgtacgg gctttgtaca
    22321 ccgttaacca gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt gtattctggc
    22381 gtaccaaagt tatccagccg tttatcctgg ataatgaccg gaaaatcatc cacaccataa
    22441 tggttgggga taggcagcga cttgctgact tcatcttcca ccagccacat tccggcaagg
    22501 ccgttataga cctgctgagc cgtgcggttg ggagtattgg cgtgatacca cagagtagct
    22561 gcgttctggc gaatgggcag tacgggtgcc cagtcagcgt ttggcgacat catccgtgcc
    22621 ggaccgccca tcagcggacc tggtacctgt agcccggcca ccgtcattga gacattttct
    22681 gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg gatagtcggc
    22741 cccaggtaac gaccattgat tccccagacc gacgcgcgtg tccctggcgt aaatgaccag
    22801 tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc gagattcaag tagcggcgga
    22861 acgggtagcg gttgctgttg cccggctgcg ctggccttca ggggaacagc gcctgcacaa
    22921 agtgcaatcc ccgatgcctg aatgaactga cgccgactga gtgacatatt tgctccatgt
    22981 aaaactggct aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac agattgccga
    23041 cttaaacttt tccggcggct tcgcgttctg cgacttcttt atcgagctcg gcgatttttt
    23101 gttccattat cgaacgacaa tgggcagcca gctcacgaac ctgatctttg ccatactgac
    23161 tgacgtcaat tggcggcagc atttcgacaa tcaccagacc gttgtgcagt cgattaagat
    23221 taatcttatt cgaagttgta gagacgcaca cgggaataat cgggacgccc gccgcaattg
    23281 ccgcgtgaaa tgctccagtc ttgaacggta gcaggccgcg accacggctg cgggttcctt
    23341 ccgggaacat ccagatggaa atacggcgtt ttttgaaatg attcacgact tccgcaatgg
    23401 tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa gttgccggtt aaccagtaca
    23461 actgcccgaa gaaggggatc cacagcaagc tctttttacc taccgtcacc gtcggcggtt
    23521 gcacaatgtt cgatgctgtc accatgtcat agttgttctg gtggttagcg atatagatag
    23581 cattgccgta gctttcagca tctgcaggtt tgcggcactc aactttcagg ccgaacagcg
    23641 gcgcaagacg gccaaacata tgcccaaagg tggccacatg tttcgggtta cgcgggctga
    23701 aaaggcagta aatggagccg aatacacaga ctaagatgct gtaaatcacg gtaataataa
    23761 gacgaaagat atatagcata acaccctctg aaggtctgag agactggcat tgtacgtcac
    23821 ctgaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat tgttaatcgc
    23881 aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg ttgaatacgc
    23941 tgccggatga cgacttaacg tttcatccgg cgttccttgc aagcgggagg aaacaggccc
    24001 tccccggcat attactcttc gctatcaccg ctgctggcac ggcgaggaga gtcgatctca
    24061 acacgatcga tacgctgcaa accgcgcatc aacgtaccgc ggcgtccacg ttcgccagtg
    24121 actttctgta actcttccgg gcgcagttta attttgcgtt tcccaacatg aatggtcagc
    24181 gtgctttgcg gcggcagaac gtacaattgc gccagaccat cttctccacg cgcggcttct
    24241 gccgatggaa tgttgataat cttgttgcct ttgcccttcg acagctgcgg cagatcgctt
    24301 accgggaaca tcaacatacg gcctgcctga gtgattgcca gcagcatatc ggaagcatct
    24361 tcaatcacca ccggcggcat aacatgggca ttttccggta aggtgatcaa agccttacct
    24421 gcacggttac gcgccaccag atcgttaaag gtgcagacga aaccgtaacc cgcatcggaa
    24481 gccatcagca gtttctgatc gtcgctttcc atcagcatat ggtcaacggt cgccccaggc
    24541 ggcaacgtta atttgccggt gagtggttcg ccctgaccac gcgccgacgg cagcgtaatc
    24601 gggtcgatgg catagctacg accggtggaa tcaacaaaca ctaccggttg gttgctctta
    24661 cctttcaccg ccgctttgaa gctatcaccc gctttataat tcaggcccgg cgcgtcgata
    24721 tcatggcctt tagcgctgcg tacccagccc atctgcgaca gcacaatggt gacaggttca
    24781 gacggcagca tgtcgtgctc gctcatcgct ttcgcttctt cgcgttcctg caacggcgaa
    24841 cgacgatcgt caccgtaggc ttgcgcgtct gcctgcagtt ctttcttcag caggttattc
    24901 attttacgct cggaagccaa aatgccctgc aactggtcgc gctctttttc cagctcactc
    24961 tgctcaccgc gaatcttcat ctcttccagt ttggcaagat gacgcagttt cagttcgagg
    25021 atcgcttccg cctgggtttc cgtaaggcca aaccgcgaca tcagcgccgg tttcggttca
    25081 tcttcgttac gaatgatctc aatcacttcg tcgatgttga gaaacgccac cagcaaacct
    25141 tccaggatat gcaggcgctt gaggactttc tccagacgat agttcagtcg gcggcgcacg
    25201 gtatcgcgac ggaacaccag ccattcggag aggatttcca gcaggttttt caccgccgga
    25261 cgaccatcca gaccgatcat attgaggtta atgcgatagc tcttttccag atcggtggta
    25321 gcgaagaggt ggttcatcac ctgatccata tccacgcggt tggaacgcgg cacaatcacc
    25381 agacgggtcg ggttctcgtg gtcagattca tcgcgcagat cgtcaaccat cggcagcttt
    25441 ttgttgcgca tttgcgcagc aatttgctcc agtacgcgcg cacctgaaac ctgatgcggt
    25501 aatgcgctga taaccaccgc gccatcttct ttcttccaca ccgcgcgcat acgcactgaa
    25561 ccacgtccgt tctcgtagat tttacggatc tcggcgcgcg aagtgataat ttccgcttca
    25621 gtcggataat ccggcccctg cacgatatcc agcagctgct cgagcgtggt tttcggctgg
    25681 tcgattaatg cgattgccgc ctgagctact tcacgcaggt tatgcggtgg aatatcggtc
    25741 gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa tgtttggcag acgagcaggc
    25801 agcattttcg gctcctgcaa agtgccgtcg aagtttggca cccagtcagc cgtcccctgc
    25861 cccagctcgc tcaatagcag ctcggaatat ttcgacaacc gggattcggt gtaacgcatt
    25921 gccgcgaacg atttcggatc gtccggcgcg ccccagttcc cctgaccatc aaccagcgga
    25981 taacggtaag agaacggctg cgccatcagg accatcgctt cataacaggc gctatcgccg
    26041 tgcggatggt atttacccag tacgtcaccg acggtacggg ccgatttttt aaatttggcg
    26101 ctggcattca ggcccagttc agacatcgca tacacaatgc ggcgctgaac aggtttaaga
    26161 ccatcgccaa taaacggcaa cgcacggtcc atgatcacgt acatggagta gtttaagtag
    26221 gcgttttccg taaattcatg tagcgcaagg cgctctgcca tatcgctcat taattctgat
    26281 tcctcaactt attcgccagc cttcaaaggg gcaatattgc cggagatact acattatctg
    26341 ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc ggacgtgctc cgacggcgat
    26401 aaaggcatca atgtgccttg atatacaact ctttgctgta gacttgatcg gcatagaggg
    26461 cagcacaagg ttgcgcccat gccacaccac tcggcatggg ggtccgctcc gggcgattcg
    26521 agaagttgag gttatgcgag tccagggaag cctaaagcat caaagtagtc gactgtaagt
    26581 atacggttca gcccagttta gctgtgtctc caccagtgcc ccgcgcctcc tgccataagt
    26641 gcggcttgct catggatacc cccgagacgg cttacggcgc accggataat aaatttcgcg
    26701 tatacatggt attccttacc ggagaaatta taagcccaac aacaatacgc ctgtggcatt
    26761 gttcgctcaa acttatcgcg agtgatttgg ctcacactcc ggtaaagatt ccccatcacg
    26821 ccaacggcac ataaacatcc gtttccagtc gctgctctgt caccccttcg gcaagattgg
    26881 tgtagtgaaa cataatcggt gctttacgca ttttctcgcc gcttgcaggc agccagtggc
    26941 gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc gccaacgtgg cgggccacgg
    27001 cataacgtcc accggtaagt tcaccattgc tcacaccata acgattatcg ggaatcggtt
    27061 cgctaacgct gccgcagata tcaaagcgaa acgcttccgg tgcggtggtg gcagggtcgt
    27121 cccaggcgac gccaaaagtc tggctttggt taacaggaga aagccccgtc tctttacgcc
    27181 acatgataaa tttcgccaca ctgtagttga gcaactcagg gctacagcgg tgcggcaaca
    27241 tcgccacagg gattgatggg aaatcgataa tgtttacatc cagtgtcagg tttgtcattt
    27301 tgcctccctg cgaaattatt cccgggaacg cgtcccggga gcggtaacaa ttacggattt
    27361 actttgcgga tctgtttgac gtcaatttca acagaattcc agtctttatc gacttcaccc
    27421 tgaatctcaa ccgtatcttt cggcgtcacc gtcacaccgt tccagcgttt gtggtcgata
    27481 tcaacattga tagtaccgct ggcatcttta aacacgtaga gatcgtcaga gatgcgttca
    27541 acgatattgc cgcgcagggt tacccaggtg tcgtcacgca gggattttgc gctttctacg
    27601 gtcgttacgc tgccgttcgg cccctggaat cctccggcct gactttgcgt tgccgatggg
    27661 ccagaaaaac cgccctgctc tgctgccatc accggtgcgc tgcacagggc cattactgcg
    27721 attactgctg cgaatttttt catgtttatt actcccttta atgtctgttt ccgagcattt
    27781 aacaagatag tccttaacaa cttcttaagg gaaaaaaata aaatttagtg ctgtacagag
    27841 cgcgttacaa cacggtttac tggcagcaaa tacggttatc gcagggatga aaaaatgcga
    27901 attttactga tagaagatga catgctgatt ggcgacggca tcaaaacggg ccttagtaaa
    27961 atgggtttta gcgtcgactg gtttacacaa ggtcgtcagg gaaaagaggc gctttatagc
    28021 gcgccttatg atgcggtgat cctggattta accttaccag gcatggatgg tcgcgatatt
    28081 ttgcgcgaat ggcgagaaaa aggtcagcgt gagccggtac tgatcctgac cgcgcgcgat
    28141 gcgctggagg aacgtgtaga agggctgcgt ctgggagctg acgattatct gtgtaaacct
    28201 tttgcgttga tagaagtcgc cgccaggctg gaagctctga tgcgccgaac caacggccag
    28261 gccagcaacg agctgcgcca cggtaacgtc atgctcgacc ccggcaaacg tatcgccacg
    28321 ctggctggcg aacccttaac actgaaacca aaagaatttg ccctgctgga attactgatg
    28381 cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag agaaactgta tacctgggac
    28441 gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc atctgcgacg caaactcggt
    28501 agtgatttta ttcgtaccgt gcatggtatt ggctacacat taggtgagaa atgaaattta
    28561 cccaacgtct tagtctgcgc gtcaggctga cgctaatctt tttaattctg gcctcggtga
    28621 cctggctgct ttccagcttt gtcgcctgga aacaaacaac ggataacgtc gatgaattgt
    28681 tcgacaccca actgatgctg tttgccaagc ggttaagtac gctcgatctc aacgaaatca
    28741 acgcggcgga tcgcatggca cagacgccaa ataaattaaa acacggtcat gttgatgacg
    28801 atgcgctgac ctttgccatc tttacccacg acggcagaat ggtccttaac gatggcgata
    28861 acggagaaga tattccttat agctatcaac gggaaggttt tgctgacggg caactggtgg
    28921 gcgataaaga tcaatggcgt tttgtctgga tgacctcacc tgatggcaaa tatcgcatcg
    28981 ttgttggtca ggaatgggaa taccgtgaag acatggcgct ggcgattgtt gccgggcaat
    29041 tgatcccgtg gctggtcgca ctgccgatta tgttaatcat catgatggta ctattgggtc
    29101 gtgaactcgc gccgctgaac aaactggcgc tggcgctgcg tatgcgtgac cctgactcgg
    29161 aaaaaccact aaacgcgact ggcgtaccca gcgaagtgcg tccgttggtt gagtcgctaa
    29221 atcaactgtt cgcccgcaca catgcgatga tggttcgtga acgacgcttt acctccgacg
    29281 cagctcacga actacgtagc ccgttaacgg cgctgaaagt gcaaaccgaa gttgcgcagc
    29341 tttctgacga tgatccgcaa gcgcggaaaa aagcgctgct tcaattacat tacgggatcg
    29401 atcgcgctac tcgtctggtt gatcaactgc tcactctatc gcggctggac tcactggata
    29461 accttcagga cgtcgcggag atcccgcttg aagatctcct gcaatcgtcg gtgatggata
    29521 tttaccacac ggcgcagcag gcgaaaattg acgtgcgact gacactcaat gcccacggca
    29581 tcaaacgcac cggacaaccg ctattgctaa gtttgttggt gcgaaatttg ctggataacg
    29641 ccgtgcgcta cagtccacag ggcagcgtga tagacgtcac gctgaatgct gataatttca
    29701 tcgtgaggga taacggcccc ggtgtgacgc cagaggctct ggcgcgaatt ggcgaacgct
    29761 tctatcgccc ccccggacaa accgctaccg gcagtggact tgggctatcg attgtccagc
    29821 gaatcgccaa attgcatggc atgaatgttg agtttgggaa tgcggaacaa ggtggatttg
    29881 aggcgaaggt aagctggtaa cttctctgtc tatgagagcc gttaaaacga ctctcataga
    29941 ttttactaat agcaaaatat aaaccgtccc caaaaaagcc accaaccaca aaccagacaa
    30001 ataccgagcg agaatacata tacccaaaac tcgaacattt cccgcataaa gagttttctt
    30061 aagataagaa taataagtgg cgtaagaaga aaaaatgctg caatcattgc ttgcaatata
    30121 cgatcttcgc ctgaaacgga aggtatactg caaatattgt ctcccgactc aagcatccag
    30181 tcatacttat tgaaagcaaa aaaagcatct attaaccaat aagtaagcat tatgtataac
    30241 agagcttcaa atattgtttg aattttatgc tttaacatat atcagtacgc tcata
//
LOCUS       DAZGLJ010000032        29956 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000032 DAZGLJ010000000
VERSION     DAZGLJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.032"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..189)
                     /locus_tag="ACHK49_001340"
     CDS             complement(<1..189)
                     /locus_tag="ACHK49_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9262014.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVS"
     gene            357..941
                     /locus_tag="ACHK49_001341"
     CDS             357..941
                     /locus_tag="ACHK49_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9262015.1"
                     /translation="MNMKNSSFLDEGKEPVIERIFRLAERYKSRSEAARAWGINVQTL
                     QNYYKRRNNQPPPQPRKHHLQNIAEREHVSMEWLVNGYGDEPSMKKEPIEQTKRTKKQ
                     RIDDSDSIIMRAWDSLTSSEQEILSNLLIRKGAELLTVLLDTDIQKLHSLSGVKRALA
                     LSLQELPDDTVREIYEECEAKVNHLNVTQKKAGA"
     gene            1216..1491
                     /locus_tag="ACHK49_001342"
                     /pseudo
     CDS             1216..1491
                     /locus_tag="ACHK49_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1491..2537
                     /locus_tag="ACHK49_001343"
     CDS             1491..2537
                     /locus_tag="ACHK49_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9262016.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDTQPTS"
     gene            complement(3027..3617)
                     /locus_tag="ACHK49_001344"
     CDS             complement(3027..3617)
                     /locus_tag="ACHK49_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO9262017.1"
                     /translation="MSEINYQALRERYSPVPVPKCPICGEEMSIQRISGAQVVYACSG
                     YGDDGDFKIGRTLADEHYEKSRVTVLDVGDPEVLALLDWLETKDNRIAELEKIATDYA
                     LKFQKAQDALKYAALLHSRTAQQTNNFAVSLPDISEYFINDVFQPLRYERDVERAIIK
                     AGGKALWQEKHEDRTHQSCDVNRGWFSPLTTDKNNT"
     gene            complement(3681..3992)
                     /locus_tag="ACHK49_001345"
     CDS             complement(3681..3992)
                     /locus_tag="ACHK49_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO9262018.1"
                     /translation="MTTPTRRISFYLKPTAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWAGDEALSVTQTRTERPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            complement(3997..4956)
                     /locus_tag="ACHK49_001346"
     CDS             complement(3997..4956)
                     /locus_tag="ACHK49_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO9262019.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNIIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            complement(5033..7873)
                     /locus_tag="ACHK49_001347"
     CDS             complement(5033..7873)
                     /locus_tag="ACHK49_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO9262020.1"
                     /translation="MSEFAWSWNEPRPAIDPARFTEHRQETETDLQRAIRYYLEADKK
                     ALEEQEAKEEAFFAQSTVGKKLMASLEEAGQREKLAQSIISKRQATEQDPVARAFATL
                     KVLPVYLREPLSRHLSFLRKKQEADRRKGKKSWQAERYARGTLRKIFERLDRTDHRWL
                     TPGYRSLAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTM
                     DVTLKAYQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRK
                     LWLLRCEWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFT
                     IDLETVYYAGVSNPVHRKAEMMATMKGLELLAEARSDRAVFLTVTCPSKYHATTENGH
                     PNPKWNGATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFA
                     HPDEIETIVSHVCDIAIQEDRHELGNDITPRFKAEYVDGSKGTPTSYIATYIGKNLDS
                     RTVDGIDPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRR
                     LASQMARNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLA
                     DELNDYGEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEPEDNAHENGFDLQGGP
                     AAPWTRGNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVNEWMRSLKRHERRALMRS
                     LRDKQAKNSSDEMQNWTQSRKQPRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLR
                     GGSLYVDGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLE
                     QLGASDPEAAAHLASTLQQDQNTMKTPVTVLSDMLRAIRDAVHAQRISETTERAHRKA
                     DLLRGSLTSGNKKQTETGLTNPVNEQKTCRDI"
     gene            complement(7870..8259)
                     /locus_tag="ACHK49_001348"
     CDS             complement(7870..8259)
                     /locus_tag="ACHK49_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HFO9262021.1"
                     /translation="MIDAHDFTRWVRTQDTRLAPVLQGLFDLYIRGRDNRARTTKPEN
                     ADTLYFTVDDCYRVDFTPHGLALHCLTPHGESLLAYYDSPASVFAAMLAHRTAGGCAS
                     LSEYTAEFNRLSALFSQKWQRVTGYQP"
     gene            complement(8332..8562)
                     /locus_tag="ACHK49_001349"
     CDS             complement(8332..8562)
                     /locus_tag="ACHK49_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO9262022.1"
                     /translation="MANRKQQRAYAARRHIQTEINRRLFRASRVAQIMHINMLHERSY
                     ALSNIYSASVFSYLADDLRELQQLFQQQNKLH"
     gene            complement(8885..9184)
                     /locus_tag="ACHK49_001350"
     CDS             complement(8885..9184)
                     /locus_tag="ACHK49_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9262023.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLNEL
                     EHARTTAPAIRLTLHHEIADFCATLEAPGEPETPEAIQQELLQRIDKVFDFFLNQ"
     gene            complement(9181..9447)
                     /locus_tag="ACHK49_001351"
     CDS             complement(9181..9447)
                     /locus_tag="ACHK49_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO9262024.1"
                     /translation="MTILDYITTHPGCSGGEIAAALNTPTTAINAELRRLWRGGLVIR
                     TNRSTGGRARKTGGQASYHANPMPFGCSNPLTHMFNQLLKEART"
     gene            complement(9444..9647)
                     /locus_tag="ACHK49_001352"
     CDS             complement(9444..9647)
                     /locus_tag="ACHK49_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9262025.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     DTYFCATNVARVNLIRQVQEPRK"
     gene            complement(9734..9847)
                     /locus_tag="ACHK49_001353"
     CDS             complement(9734..9847)
                     /locus_tag="ACHK49_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9262026.1"
                     /translation="MSIFISWLVLIISVACAIGIMRIINSVKKIERFFTGE"
     gene            complement(9844..10086)
                     /locus_tag="ACHK49_001354"
     CDS             complement(9844..10086)
                     /locus_tag="ACHK49_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO9262027.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            complement(10098..10376)
                     /locus_tag="ACHK49_001355"
     CDS             complement(10098..10376)
                     /locus_tag="ACHK49_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9262028.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGVVPSEKNAATYVYLN
                     GTRLTVELPYQTVHGIISEAEKARKINGDEPYIEIICMDSEAEIQKAD"
     gene            complement(10387..10725)
                     /locus_tag="ACHK49_001356"
     CDS             complement(10387..10725)
                     /locus_tag="ACHK49_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO9262029.1"
                     /translation="MLKQRRNFCTGTERQANRFATSASRSNIRYSLSETHATPDGHTV
                     KQIGEHTWLIEKAGIVVHRCQRNPFTGNRIFALSNGDNQFGQDFTLYEALRTVDRLLR
                     GQSFIKQTDL"
     gene            complement(10740..11018)
                     /locus_tag="ACHK49_001357"
     CDS             complement(10740..11018)
                     /locus_tag="ACHK49_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO9262030.1"
                     /translation="MTKLLNTYEQADFERLAAFYPYRDEHGLPVLEESLKDYAKRTNQ
                     TVNAVKRQADRAALPINQEEKNSKRTVNLFAIFLKTIRNAEKYVQMTK"
     gene            11110..11421
                     /locus_tag="ACHK49_001358"
     CDS             11110..11421
                     /locus_tag="ACHK49_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9262031.1"
                     /translation="MSSGYEKKLKEIRKSEGLTQAEFADVTGINLGTIKNYESGKREV
                     GLSVVDRVINSKDFEKYTIWLMTGKTNEAAGQISPSLSPDGPENTSSSQKSRKTGTQP
                     G"
     gene            11510..12463
                     /locus_tag="ACHK49_001359"
     CDS             11510..12463
                     /locus_tag="ACHK49_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9262032.1"
                     /translation="MRPEGRKGKRIRKKFKTKSDAVLYERWVLAQQHNNEWKGNSIDR
                     RPLSVLIDLWWKYHGQLMKSGHNTRLKLLRLSEAMDDPCVHKLNTTMLTELRVSRIEQ
                     GIQPSTINREIGALSAMFTALISSGHFLNDNPVQGLKGMKVNEREMGYLSKSECVQLL
                     DALAENPDERLAVEILLSTGARWGEVAALEQRRVLHCRITFSKTKNSKNRTVPISESL
                     FEKIKKRGGKLVFPTLDYSLVRDVIKTVAPDVPDGQAVHALRHTFASHFMMNGGNILT
                     LQKILGHAKIQTTMIYAHLAPDYLQDAVRFNPLGDALYEAS"
     gene            12942..13379
                     /locus_tag="ACHK49_001360"
     CDS             12942..13379
                     /locus_tag="ACHK49_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HFO9262033.1"
                     /translation="MSDKLERRIERLEGDLSLTRNDLATLAERTTNLSTKADVGEVKG
                     ELKADIAHLKGDLKCDIANLKGELKSDTAHLKEQLKSDINSLKGELTEAMDKRFDKIM
                     DEMNRRFDKVDDNTKWRWSGIIVPVCTTIFTAAVAVFVAKFVG"
     gene            13572..14552
                     /gene="btuC"
                     /locus_tag="ACHK49_001361"
     CDS             13572..14552
                     /gene="btuC"
                     /locus_tag="ACHK49_001361"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO9262034.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            14615..15166
                     /gene="btuE"
                     /locus_tag="ACHK49_001362"
     CDS             14615..15166
                     /gene="btuE"
                     /locus_tag="ACHK49_001362"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO9262035.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            15166..15915
                     /gene="btuD"
                     /locus_tag="ACHK49_001363"
     CDS             15166..15915
                     /gene="btuD"
                     /locus_tag="ACHK49_001363"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO9262036.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            15993..16457
                     /gene="nlpC"
                     /locus_tag="ACHK49_001364"
     CDS             15993..16457
                     /gene="nlpC"
                     /locus_tag="ACHK49_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO9262037.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            16704..17417
                     /gene="ydiV"
                     /locus_tag="ACHK49_001365"
     CDS             16704..17417
                     /gene="ydiV"
                     /locus_tag="ACHK49_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO9262038.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            17480..18916
                     /gene="selO"
                     /locus_tag="ACHK49_001366"
     CDS             17480..18916
                     /gene="selO"
                     /locus_tag="ACHK49_001366"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO9262039.1"
                     /translation="MTLSFITRWRDELPATYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSYLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(18920..19111)
                     /gene="hemP"
                     /locus_tag="ACHK49_001367"
     CDS             complement(18920..19111)
                     /gene="hemP"
                     /locus_tag="ACHK49_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO9262040.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(19243..20289)
                     /gene="aroH"
                     /locus_tag="ACHK49_001368"
     CDS             complement(19243..20289)
                     /gene="aroH"
                     /locus_tag="ACHK49_001368"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO9262041.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLEMARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNSRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(20446..21279)
                     /gene="ppsR"
                     /locus_tag="ACHK49_001369"
     CDS             complement(20446..21279)
                     /gene="ppsR"
                     /locus_tag="ACHK49_001369"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO9262042.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKLLDIMGLSRRM
                     Y"
     gene            21612..23990
                     /gene="ppsA"
                     /locus_tag="ACHK49_001370"
     CDS             21612..23990
                     /gene="ppsA"
                     /locus_tag="ACHK49_001370"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO9262043.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(24047..25693)
                     /gene="fadK"
                     /locus_tag="ACHK49_001371"
     CDS             complement(24047..25693)
                     /gene="fadK"
                     /locus_tag="ACHK49_001371"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO9262044.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(25750..26043)
                     /gene="ydiT"
                     /locus_tag="ACHK49_001372"
     CDS             complement(25750..26043)
                     /gene="ydiT"
                     /locus_tag="ACHK49_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO9262045.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(26040..27329)
                     /gene="ydiS"
                     /locus_tag="ACHK49_001373"
     CDS             complement(26040..27329)
                     /gene="ydiS"
                     /locus_tag="ACHK49_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO9262046.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCIMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(27385..28323)
                     /gene="ydiR"
                     /locus_tag="ACHK49_001374"
     CDS             complement(27385..28323)
                     /gene="ydiR"
                     /locus_tag="ACHK49_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO9262047.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(28343..29107)
                     /gene="ydiQ"
                     /locus_tag="ACHK49_001375"
     CDS             complement(28343..29107)
                     /gene="ydiQ"
                     /locus_tag="ACHK49_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO9262048.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            29423..>29956
                     /locus_tag="ACHK49_001376"
     CDS             29423..>29956
                     /locus_tag="ACHK49_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFO9262049.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKK"
BASE COUNT         7078 a         7310 c         7850 g         7718 t
ORIGIN      
        1 cgatacccca agagcatcag caattatttg ctgatacggt cggcaaggag tacgtaatac
       61 gcttttcagc gaatccctgc tgtaaccagc ctttaaagat aaagagcgca gtgataagcc
      121 agccatgtgt aagcgcgcct tgatgatttc aggcggccaa tcgtgatctg aaacttctct
      181 tctactcata cttcctctat tctaaaaagt tacccgcgcg gatattcgtg cggatatctg
      241 tacgggtaaa taatagaaca aacaaaagaa ccaaatcaag gtattttgtt ctttttattc
      301 tttgcggtgt ttttgttatt aatatcaata agataaatac aaaggaacct gagagcatga
      361 acatgaaaaa cagttctttt ctcgacgaag gaaaagaacc agtgatcgaa cggatttttc
      421 gtcttgcaga gcgttacaaa tcgagaagtg aggcggctag agcatgggga ataaacgttc
      481 agacattgca gaactactac aaaaggagaa acaatcagcc gccaccacaa ccaagaaagc
      541 accatctcca gaacattgca gaaagagaac atgtatctat ggagtggcta gtcaatggtt
      601 atggtgatga gccaagcatg aaaaaagaac caattgaaca aacaaaaaga acaaaaaaac
      661 aaagaattga tgatagtgat tccatcatca tgagggcgtg ggactcgcta acttcatctg
      721 aacaagagat tttgtccaac ttattgatca gaaaaggggc cgaactacta accgttttgc
      781 ttgatacaga tattcaaaaa ctgcactcat tgagcggagt caaaagagca ctagcattga
      841 gtctacaaga gttacccgat gatactgtaa gagagattta tgaagaatgt gaagctaagg
      901 ttaatcattt aaatgtaacc cagaaaaagg ctggtgcgta agggagtggt gcaatttttg
      961 tgctgtagta cactccttta aagacaatct ggagaaaaac gttttaagcc tttttaaagc
     1021 cattttaagc gtcaatccgt agtatcaaaa gttttgccga ttttagaata aacgattaat
     1081 tattgcacaa tcggtatcaa atagacttta agcgctcatc cagacatcac ctgttttatt
     1141 tcagtatctt acaggtgatt tcacctcctt tcactaaaac cctcgttggt atcaaatgat
     1201 tcacctggtt acaacacctt cacgctgtca tcaaaacggt cgctggtact gaaaatgctg
     1261 caaataatgc gtgccgggcg gtgggaatac gatcgcgccg aacgcgagct ggtcgcggcc
     1321 tttaacgccg tgcgtaaggt gaaaacaccg ggcggcttta tcacttacga aacggaccgc
     1381 gcgaggggga tcagccacgg cgaccttgcg tgggcaacca tgcttgctgt cattaacgaa
     1441 ccgattggcg gcgaaggaga aaacgagcgt ttcacggtta tggagttctg atgagcagaa
     1501 aaaataaaaa agtgcgcatg agttcacgca ttgatctcgc tgatgcgctc aggaaagaat
     1561 catcgctcag tgcattcaca tttgatggtc cttatcgcct gaccgggcat gacctgctgg
     1621 acaatatgta ctgtgctgat aacggacggt ggtatgaaac cccggtggac tggtacggtc
     1681 tggcaagagc agcccggcaa acgtcctggc atcagtctgc gctttacttt aagcgcaatg
     1741 tattactcgg ttgctacatc ccgcacccgc tgctttcccg gcaggatttc tcggcgctgg
     1801 cgctggactg gtttgtgttc ggtaacgcat tccttgagct tagaagcaat atgctcggcg
     1861 aaccgcttaa attacggcac gccctggcga aatacatgcg acgcggaagc gatcttgaat
     1921 catggtggta tgtgcaggat ggcaaggacg cgttccagtt tcgccctggc aaagtgtgcc
     1981 acctgatgaa tccggatatt aaccaggaaa tctacggcat gccggaatat cttggcgcat
     2041 tactctcggc cagcctttct cattcggcgg acatgttcag aaaactgtac tacgacaacg
     2101 gatcccacgc cgggtgcatc atctacatcg gtgcagcgca ggtaaaccgc gaaagcatgg
     2161 actccctgaa agaaacgtta cagggcgcgc gtggtggtgg tgcgtttaaa aacgtactca
     2221 tccatgcgcc caacgggggc aaagaaggtg tgcaaatttt gccgttccag cagatcactg
     2281 caaaggatga gttcatgaat gttaaggcgg catcccgtga tgatgtgctg gctgcgcacc
     2341 gcgttccgcc gcaactgatg ggggcgatgc cgggcgaaaa aagtgcgttt ggtgatgtgg
     2401 agaaggccgc gcgggtttac gcaattaacg agctgatgcc cgtcatggag gccatgaagc
     2461 acatcaatga ctggcttggc gaagaggtga tccgctttaa cccttacgca ctgctagata
     2521 cccagcccac atcctgacgc gcttcgcttg tctgctgctt cgccggggca taaaaaattt
     2581 atgccccgac tctccagctc ctgtatcaat cagataattt cacgacgctt ttctgcttat
     2641 tgtcatcatc ggcggtcaga ctcttacgca atcccaccgc gttgactgca tgttctcgcc
     2701 gcctcagtgc gattttgacg gccttatctg ccacccatca aatcaaaagc cctcacgtct
     2761 ttttcacgct cagcgtgaga aatacagcca ttctgttgtg tcgctgcgac atcgttcagg
     2821 ggatgctatt taccccctga aacgcgggct gttcccccgt cacctgcgcg cagaaaaagc
     2881 gcgttttttt gtgcacgcac ggatccttga cggatccagc cgccatgcgg gccggaaggg
     2941 taaaaagtcg ttcaaaaaaa ttgtgcaaac ttgtgcacta ttgtgcagca gggcgatgcg
     3001 cgattatcgc cctggtttga gggggatcag gtgttatttt tatctgtcgt cagtgggcta
     3061 aaccatccac gatttacatc gcaggactga tgcgttctgt cctcgtgttt ctcctgccac
     3121 aatgcttttc cgccagcctt tatgatggct ctttcaacat cccgctcgta tcgcaagggc
     3181 tgaaatacgt cattaatgaa atattcgctt atgtccggaa gcgatactgc aaaattattt
     3241 gtttgttgcg ccgtcctgct atgcagcaaa gcggcatact ttaatgcgtc ctgtgctttc
     3301 tggaatttaa gtgcatagtc agtggcgatt ttttccagtt cagcgattcg gttgtctttg
     3361 gtttccagcc aatcaagtag cgccaatact tcaggatccc cgacatccaa cactgttacg
     3421 cgtgattttt cataatgttc gtcggcaaga gttcggccaa ttttgaaatc tccatcatca
     3481 ccataaccgg agcaggcata aacaacctgt gctccagata ttcgctgaat tgacatttcc
     3541 tcgccgcaaa tagggcattt cggtactggc acaggtgaat aacgttcacg taatgcctga
     3601 tagttgattt cgctcattaa gtcgttctcc ttgtttatgt tctgaggtgg agagcagggc
     3661 gaactcgccc tgatgttgtg ttattcagga aataacgccc ggatatttcc ggccatctga
     3721 ctggttatct gtgcggttga tactggctgt gatgcggggc gttctgtcct ggtttgtgtc
     3781 actgataacg cttcatcacc agcccatgca gccagtcgat aagcctctgc cggattcatt
     3841 ttcagaagtg ccagcccggc cagaaaagcc acgcgttggc cgcttttgcg ggcttctggt
     3901 gtaaggctgt ccagccaggc gcatgcttct ccttcgttct tgacggcggt gggcttcaga
     3961 tagaaactta tccgtctggt tggagtcgtc attggtttac tccttgtcca ttgcgtacag
     4021 cccattaacc agagcaaact gtggcacccc gtccgcgatg aaagtcgcat taactccgca
     4081 ggcttcgcgg atagcgggtg ccacaatctc cgcccctcca ccgacaacca tcacccgccc
     4141 gtaacccgaa aaacccgcca gcgcgcggat cacgcgttgt ttcagtgttt cttccttttc
     4201 acgaataacc gccatcaggc tggcgtaatg cgcgtcattg tggatgtgct ggcgcagcca
     4261 ggcttcatca tggcgatgtt cgataatggt attggcgatg tggtgactgg tgcgcatacc
     4321 gttagtggcc atcaccgaca gtacggcatc ggccatcaga gaaacgccca cgtgtggatc
     4381 gcaaaacacc tggctgatac ctgccagttg cccctgaacc tttgccacat ccagagtggt
     4441 tccgcccaaa tccacaatca gcagggattc aaacggactc atgtcagcca gtgccttaaa
     4501 gccagccgga atggattcag gcataacccg tacgttacgg atagtgaatg cttcgccgtt
     4561 ctggtactcc accgggcgca tgacgttcgc ttttttgcgg ttgatgttgg ccatgtccgg
     4621 ctgtgcgttt gtatcgaaat actcgctcag tggcagggtg acaaccacat ccacttcctg
     4681 tggtgtgatg cctgatttga ccagcgcgtg atgaatggca attatattca catcgctgta
     4741 ctggtattgc gtgtcggtcg tctggacaaa gcgatcgctg accggatcaa aaccatagcg
     4801 cacgccatca agcatgtagt tcgcgggctg cgtgccaccg aacggcgcag accattccga
     4861 cttgaagctg ttcgggctga tggcgttgcg gcgttcgccg ttctcagtcc atgccagctt
     4921 gatgttggtg gagccgtcgt cgatacaaat tttcatgtcg cttttccttg tgttgattaa
     4981 ttaatcggtt acaggatttt taaatcccgc ttttgcctgt tttgtgcgcg cttcatatat
     5041 cgcggcacgt tttttgctca tttacgggat ttgtgagtcc cgtttctgtc tgttttttgt
     5101 ttccactggt caggctaccc cgcagcaggt ctgctttgcg gtgggcgcgt tcagtggttt
     5161 cactgattct ctgtgcgtgc actgcgtcac ggatggcgcg cagcatgtca gaaagcacgg
     5221 taacgggggt tttcatggtg ttctggtcct gctgaagtgt ggatgccagg tgtgcggcgg
     5281 cttcggggtc tgatgccccc agctgttcca gatagctggc gaccgggtta tggcggatct
     5341 ccgtgctgct tacgccgtga ttacggctca ggcgctgcca gagttgcgtg attcggctgt
     5401 ccgggcgggt atctggtttg cgtacaattt caaatccctg cggtgcaatg atgctgccgt
     5461 caacgtacag actgccgccc cgtaacaggt gctgcatctg ctgttcaccg atatgcaggc
     5521 cgagagattc agcagactcc cgccattctt tagcgagtaa ctcgtggtta tcaggcaaag
     5581 gccgtggctg tttgcggctc tgtgtccagt tctgcatttc atcactgctg ttttttgcct
     5641 gtttgtcacg cagcgaacgc atcagcgccc ggcgttcgtg ccgtttcagt gagcgcatcc
     5701 attcattcac gtcaacgccg tcaggaagct gcggccacgg tgctggcctt tcttccggct
     5761 gttctgtccc gttgttgtcc gtttcctgta cacggggaca gttattgcca cgagtccaag
     5821 gggcggcagg gccgccctga aggtcaaaac cattttcgtg ggcgttgtct tctggttctg
     5881 gtttacgtct taccaacttc cagttatccg gatgcgtgca cacacgggag gattccccga
     5941 tgagtggtga ccagatcccg taaatctgta cgctctgttc gccgtaatcg ttcagctcat
     6001 ctgcgaggtc gtaggcggtg cgaatcaggt agtccttgcg tggaacaagc acgccaccct
     6061 gtttttcaat gtaggtggca aaacacccgg catcggcggc agcgagtacc gcatccattg
     6121 catcatcttt cagccgttgc gggccttccg gattgcgtgc catctggctg gcaaggcggc
     6181 gcagttcacg ccacacctga cgggagggga tgccaaagaa ctggaactga cggacccggt
     6241 gaaggcgcgc ccagccgatg gcgcgctcca cgctctcggc cattgattta cctgtttcgt
     6301 ggtcaacgcg tggcttgccc gttttcgggt cgatgccatc cacggtgcgg ctgtccaggt
     6361 tctttccgat gtaggtggcg atgtagctgg ttggcgtgcc ttttgagccg tcgacgtact
     6421 ccgccttaaa acgcggagtt atgtcattgc ccagctcgtg gcggtcttcc tgaatggcaa
     6481 tatcgcagac gtgggacacg atggtttcaa tctcgtccgg atgtgcaaag accatcatat
     6541 gccagtgcac agtgccgtca tggtgaggct ccaccgtgcg gatgccatac cagcgcaggc
     6601 cgtcgcggtt cagttttttg cggaccgccg caaaaaacgt gttaaccagg taatcgctag
     6661 agtcgcgcat ggtggccccg ttccatttgg ggttcggatg accgttctcc gttgttgcgt
     6721 ggtattttga cgggcaggtg acggtcagaa acaccgctct gtcgctacgg gcttcggcca
     6781 gaagttccag tcccttcatg gtggccatca tttctgcctt acggtgaacc gggttactta
     6841 ctcccgcgta atacaccgtc tcgagatcaa tcgtgaaccc gtcttcattt tccagcatga
     6901 aacttttcag gaaatcgcgt gttttctcgc gctgtgcgcg aaactcgctt aacgcgtcct
     6961 ggctcagata gggcgatgtt tttctggaaa ccagacaggc ggcgcggagt tgttcttccc
     7021 gccactcgca acgtaacagc cacagtttgc gtttccacca ttccgcacag gtcaggcgaa
     7081 ggattgcgcc cggcagcagc tccgtgtctg gttcgttcct ccggtctttg tctgttgtca
     7141 gtgcgtcata atgtggaggc atggcgtgca ggtgtaacgc catgcgggcc agcatctgat
     7201 acgccttcag cgttacatcc atggtcagct cgccatcggt cgcgccaaag ccatcgcaga
     7261 gtttttcgaa ggtgctgctg aacatcgccg ccgtcatggt ggccagcgtc tgtatctggt
     7321 gtttgttgag ctgcggcagg taaagcaaat cgtccaggcg ttcgcgtccg gcaagggagc
     7381 gataacccgg tgtcagccag cggtgatcgg tgcggtccag acgttcgaat attttgcgca
     7441 gggttccgcg cgcgtagcgt tccgcctgcc agctcttttt gcctttccgg cgatcggctt
     7501 cctgtttttt gcgcaggaaa gagaggtggc ggctcagagg ttcacgcaga taaacgggaa
     7561 gcaccttcag tgtggcaaaa gcacgggcca ccgggtcttg ttctgttgcc tgacgcttgc
     7621 tgatgatgct ttgtgccagc ttttcacgct gtccggcttc ctcaagggat gccatgagtt
     7681 ttttacccac ggtggattgt gcgaaaaagg cttcctcctt cgcttcctgt tcttccagag
     7741 cctttttgtc tgcctcaagg tagtaacgga tggcgcgttg caggtcggtt tcagtttcct
     7801 gcctgtgctc cgtaaatctg gccggatcaa tggctggccg tggttcattc cagctccatg
     7861 caaactcact catggctggt atcccgtcac gcgctgccac ttctgcgaga agagggcaga
     7921 aaggcggtta aattcagcgg tgtattcact cagcgaggca cacccgccag cagtgcgatg
     7981 cgccagcatt gccgcaaata cggaggccgg ggagtcgtaa tacgccagca gtgattctcc
     8041 gtgtggtgtc aggcagtgca acgccagccc gtgtggtgtg aagtccacgc ggtagcagtc
     8101 gtctactgtg aaataaaggg tgtctgcatt ttccggtttt gtggtgcgtg ctctgttgtc
     8161 acgaccacgg atgtagagat caaataatcc ctgaagaacg ggagccagac gggtgtcctg
     8221 tgtgcgcacc catcttgtga agtcatgagc gtcaatcatg ctgcaattct ctttactaca
     8281 gatgtgcgaa ggcctcccgc cgcaaggtgc aggaaaggcc cggaacagga attaatggag
     8341 tttgttttgc tgctggaaga gttgttgcag ctcgcgcaga tcatccgcca gatagctgaa
     8401 aacagaggcg gaatagatgt ttgatagtgc gtagctgcgc tcatgcagca tattgatgtg
     8461 catgatttgc gcgacgcgtg atgcgcggaa aagtctgcgg ttgatttcag tctggatgtg
     8521 acgacgcgca gcgtatgcgc gctgttgttt gcggtttgcc atggtgtggc ctctgtagtt
     8581 gcaagttttg aaaactcacc atccagaggt ggaaaactcg gggtggtgag acgtacaggg
     8641 tttccacaac cggcaactac agaacccggc ccgaccgaag tcggccccgt acgccccacc
     8701 ataattcgtg tgcgaaaaag acgtggcgat acagtacgca caaaaaaacc gctggcgcgg
     8761 ttgtgcgctg tagttgtcag cggggtggaa atcccggcac ccgttttatg aggtgcagcg
     8821 gaaatgtaac ctgactgatt gcggcatggc aagcggtttt tttgtgtgtg catgttctgg
     8881 tttcttactg gttcagaaaa aaatcaaaaa ccttgtcaat gcgttgcagc agctcttgct
     8941 gtattgcttc cggcgtttcc ggttcgcccg gcgcctccaa cgtcgcgcag aaatcagcga
     9001 tttcatgatg gagcgtcagg cgaatggcag gggccgtggt tctggcgtgc tccagctcat
     9061 tcagcagtgc cagcacagca gacggcgaga gcattgcgcg aaatgccagt aatttttgag
     9121 gcgttgccat tcgttgcagg gcaaatgcca gttcgcgtag cttctggtgg ttgatggtgc
     9181 tcatgttctg gcttccttca gtagctggtt aaacatgtga gtaagtggat tgctacaccc
     9241 gaacggcatc gggtttgcgt ggtaagaagc ctggcctcca gttttgcgag cgcgaccacc
     9301 tgtgctgcgg tttgttctga tgactaagcc gccgcgccaa agtcggcgta actcagcatt
     9361 aatagctgtg gttggggtgt tcagtgctgc ggcgatttct ccgccgctac aacccggatg
     9421 tgtagtgatg tagtccagaa tggtcatttg cgtggctcct gtacctgtcg gataagattc
     9481 acccgcgcca cgttggtggc gcagaagtaa gtgtcgtcag tgaggtagat gtggtgtgca
     9541 tccttttccg aacggtgttt gtcgattgtg gtaatcaggc gttcgtcgac ttcgtattca
     9601 cgtcctctgg aggtaaaacg aacgacagga aaatgcttaa ttgccattac gcctccttgg
     9661 cgtgtgtgaa tacctccgcg aatgcggatt gtttttacat tttcttattt aacctgtggt
     9721 tttatttgcg ctgttattcg ccagtgaaaa agcgttcaat ctttttcact gaattaataa
     9781 ttcgcataat cccaatggcg caggccaccg aaataatcag aacaagccat gagataaata
     9841 tactcatgcg atatttccca gcttatacgg ttcaatatgt tccccgcatt ctgcggcaca
     9901 gatcagctcg gaaagttcgt taagtgcatc cagatcatca gcgtaaaaag ccacgtcata
     9961 cagacttcgg attgctctgg tcaatgagtc acgggccgca cgttcagcat gagcgcctga
    10021 tgcacttaag cgaaaataaa aacgctcaag tgctttgtta atgagagttt tatattcttt
    10081 gcccatcgca acgcccttta atctgctttc tgaatttcag cttctgaatc catacaaata
    10141 atttcgatat agggttcatc gccattaatc ttacgtgctt tttcagcttc gctaatgatt
    10201 ccgtgtacag tctggtacgg aagttctacg gtcaggcgcg tgccgttcag ataaacgtaa
    10261 gtagctgcat ttttttctga tgggacaact ccatcaatag ctgatgcgcg caataacagt
    10321 tcaccgcgaa aatcaataaa acggataaat acaccttgtg catgctcttt ggtcataacg
    10381 cacctgttat aaatcagtct gtttaataaa actttgcccg cgaagcagac gatcaaccgt
    10441 gcgaagtgct tcgtacaatg tgaaatcctg tccaaactga ttgtcgccgt tgctcagagc
    10501 aaaaatgcgg tttccggtaa atgggttgcg ttggcatctg tgaaccacga ttccagcttt
    10561 ttcaatcagc caggtgtgtt cgccaatttg ttttactgta tggccatctg gcgttgcgtg
    10621 tgtctcgctc aggctgtagc ggatgttgct gcgtgatgcg ctggtagcga aacggttagc
    10681 ttggcgttct gttccggtac aaaaattacg gcgttgcttc agcataaaat gacacctcgt
    10741 tattttgtca tctgcacgta tttctctgcg tttctgatgg ttttcaggaa aattgcgaag
    10801 agatttactg tacgttttga gtttttttct tcttggttga tgggaagggc tgctctgtca
    10861 gcctgccttt tcactgcatt aacagtttga ttggtacgct tcgcgtaatc tttcaggctt
    10921 tcttcgagta ccggtaatcc atgctcatcg cggtatgggt agaacgccgc caaacgctca
    10981 aaatccgctt gctcgtatgt gttcaagagc tttgtcatga tgtgataacc tgttcagtct
    11041 gtgtttattt gttgctaaaa gtcgtctcta ggcgactttt agggttaatt tagtcgtctg
    11101 gagactacca tgtcaagtgg gtatgaaaaa aaactgaaag agatacggaa aagtgaaggg
    11161 ttaactcaag ctgagtttgc tgatgttact gggataaatc tcggaactat aaagaattat
    11221 gagagcggta aaagagaggt tggtttaagc gttgttgatc gcgtaattaa ttctaaggat
    11281 ttcgaaaaat acactatatg gcttatgacg ggaaaaacaa atgaggctgc tgggcagatc
    11341 agtccttctc tctcccctga tgggccagaa aacacatcgt cttctcaaaa atcccgcaag
    11401 actggcacac agcccggcta atcatggaac gctgggggca tggtggtctt gtaacgctgg
    11461 ggcttcacga atgagcataa aatcaattcc gggagggtat cttcttgaca tgcgtcctga
    11521 ggggcgtaaa ggcaaacgca ttcgcaaaaa atttaaaacg aaatcggatg cagttttata
    11581 tgagcggtgg gtgctggcgc aacagcataa caatgagtgg aaaggaaact ccattgatcg
    11641 ccgtccgctg tcggtgctta ttgacttgtg gtggaaatac cacggccagc taatgaagtc
    11701 agggcataac acgcgcctta aattgctgcg cttgagtgag gcaatggatg acccgtgtgt
    11761 gcataaactt aatacaacga tgctcaccga gctacgtgtg tccaggatag agcaggggat
    11821 acagcccagc accattaatc gagagattgg ggcgttaagc gcgatgttta ccgcactcat
    11881 ctcatccggc cattttctta acgataaccc cgttcaaggc cttaaaggaa tgaaggttaa
    11941 cgagcgcgaa atgggatacc tgagtaagtc tgaatgtgtt cagttgctgg atgcactggc
    12001 tgaaaatccc gatgaacggc tggctgtcga aatccttctg tcgaccgggg cgcgatgggg
    12061 cgaggtagcg gcactggagc agcgccgtgt tcttcattgt cgaatcactt tttcaaaaac
    12121 gaagaacagc aaaaaccgta ccgttcctat ttctgaaagc ctgtttgaaa agatcaaaaa
    12181 acggggcggg aaactggtgt ttccgacgct ggattattca ttggttcgcg atgtcatcaa
    12241 aacggtcgca cctgatgttc ctgacggcca ggctgttcat gcgctgcgcc acaccttcgc
    12301 cagtcatttc atgatgaacg gcggcaatat tctgaccctc cagaaaattt tggggcacgc
    12361 aaagattcag acaacgatga tttatgccca tcttgcgccg gattacctgc aggatgcggt
    12421 gagatttaat ccccttggag atgccttata tgaagcctct taaatttgat gattttccat
    12481 atgcagacag gtctcttaat gataatgaac gtgggcattt gcaggttctg caacagattt
    12541 cgcccaaatt atttattcaa ttcctcaaag atagaggagc cagaacctct tgtctctcgt
    12601 gtgggcgccc agatttgttt attccacata cggttgttca cggcacagac cctgaacttg
    12661 atgattatga tgattcaaat gattgggaat atgtcactcc catacacaag gagaacgagc
    12721 ccattaacat ctacaatact cggtatgaag tgtcatgttc ttattgcggc ttcacatcca
    12781 catatacagc ccacacagtt gtttgttggg caagagacaa aggatatata gtttgggagg
    12841 gaatctgagt ggcatatgct aaaagtgagc atggcataac ccttattcgt gaggacattg
    12901 gcaattcttt cgatggggga ggcggtgata gtggaggcgg aatgtcagat aaacttgaaa
    12961 ggcgaattga gcggctcgaa ggtgatttat cgctaacaag aaacgacctt gcgacgcttg
    13021 ctgaacgcac tacaaacctc tcaaccaaag ccgatgttgg tgaggtgaaa ggtgagctca
    13081 aagcagacat tgctcatctg aaaggtgatc ttaaatgcga tattgcgaat ctgaaaggtg
    13141 agcttaaatc tgatacagct cacctgaaag agcagctcaa atcagacatt aacagcctga
    13201 agggtgagct taccgaagcg atggataaac gctttgacaa gattatggat gagatgaatc
    13261 ggcggtttga caaggttgat gataatacga agtggcgttg gagtggcatt attgtgccag
    13321 tatgcacaac cattttcacg gcggcggttg ctgtatttgt tgctaaattt gttggctgat
    13381 gatccacaaa ttgaccacat ctctgttatt tgttgtggtt ggctgtgttt ttgtgtgtct
    13441 gtaagtcttt gataattatc taacttgttg atttttgttt gtgtttatgg ccgctctgcg
    13501 gccttttttc ttttcactgt cgaagagtca ccgtaaaatc aacgccatga cacttcagca
    13561 gaacggatac catgctgaca cttgcccgcc aacaacagcg acaaaatatt cgctggttat
    13621 tatgcctgtc agttttgatg ctgctggcgc ttctcttaag cctttgcgcc ggtgaacaat
    13681 ggatctcgcc aggtgactgg tttactcctc gtggcgaact gttcgtctgg caaattcgcc
    13741 tgccacgtac gctggctgta ttgctggttg gtgcggcgct ggctatatcc ggcgctgtaa
    13801 tgcaggcgtt gtttgaaaat cctctggcag aacctggact acttggcgtc tctaacggcg
    13861 caggcgtggg gcttatcgcc gcggtattgc ttgggcaagg gcaactcccc aactgggcgc
    13921 tagggctgtg tgcgattgct ggcgcgctta tcatcacttt aatactctta cgtttcgccc
    13981 gtcgtcatct ttcgaccagt cggttattgc tggctggcgt tgcattaggg attatctgta
    14041 gcgcactaat gacgtgggct atctactttt ccacctctgt tgatttacgt cagctgatgt
    14101 actggatgat gggcggtttt ggcggcgtag actggcggca aagctggctg atgctggcat
    14161 tgatccccgt gttgttgtgg atctgttgtc agtccaggcc gatgaatatg ttagcacttg
    14221 gcgagatctc ggcgcggcaa ctgggtttac ccctgtggtt ctggcgcaat gtgctggtgg
    14281 ccgcgaccgg ctggatggtt ggcgtcagtg tggcgctggc gggtgctatc ggctttattg
    14341 gtctggtgat cccacatatt cttcggttgt gtggtttaac cgatcatcgc gtattacttc
    14401 ccggctgcgc gctggcaggg gcgagcgcat tgctgctggc cgatattgta gcgcgcctgg
    14461 cattagctgc cgcagagctg cctattggcg tggtcaccgc aacgttgggt gcgccggtgt
    14521 ttatctggtt attgttaaaa gcaagacgtt agccgcaaaa agacggtcta tgattaaaag
    14581 ctaagatttt tactgaccac acccaggaga aacgatgcaa gattccattc tgacgaccgt
    14641 agtgaaagat atcgacggtg aagtgaccac gctggagaag tacgctggta atgtgctgtt
    14701 gattgtcaat gtcgcctcaa agtgtggctt aacgccgcaa tatgagcagt tggagaatat
    14761 tcagaaagcc tgggccgatc gaggttttgt ggtgctggga ttcccgtgca accagtttct
    14821 ggaacaagaa ccgggcagcg atgaagagat taaaacttac tgtaccacca catggggggt
    14881 gacgttcccg atgttcagta agattgaagt taatggcgaa ggacgccatc cgctgtatca
    14941 aaaattgatt gccgcagcgc cgaccgcagt cgcgccggaa gagagcggat tctatgcccg
    15001 tatggtcagc aaaggtcgtg caccgctgta ccctgatgat attttatgga attttgagaa
    15061 atttttagtt ggcagggacg ggttggtcat ccagcgtttt tccccggata tgacgcctga
    15121 agatcccatt gtgatggaaa gcattaaact ggcgttggca aaataatgtc tattgtgatg
    15181 cagttacagg atgttgcgga aactacccgc ctggggccgc tttctggcga ggttcgggct
    15241 ggggagatcc tgcacctggt ggggccgaat ggcgcgggta agagtacctt gcttgcacga
    15301 atggccggaa tgaccagcgg taagggaagc attcagttcg cggggcaacc actggaagca
    15361 tggtccgcaa caaaactcgc gctgcatcgc gcctaccttt cacaacagca gacgccgccg
    15421 tttgcaatgc cggtctggca ctacctgaca ctgcatcagc acgataaaac gcgtaccgaa
    15481 ctactgaatg atgtcgcagg ggcgctggct cttgatgaca aactcggacg tagcaccaat
    15541 caactttccg gcggtgaatg gcaacgcgta cgtcttgctg cggtggtgct gcaaatcaca
    15601 ccacaagcca atcccgcagg tcaattgctg cttcttgatg agccgatgaa cagtcttgat
    15661 gttgcgcaac aaagtgcgtt agacaaaatt ctgagcgctc tgtgtcagca aggactggcg
    15721 attgtgatga gcagtcacga tctcaaccac acattgcgtc atgcgcatcg ggcgtggttg
    15781 ctaaaaggtg gaaaaatgct ggccagtgga cgcagagaag aggtgctcac gccgccaaat
    15841 ctggcgcagg cctatgggat gaattttcgc cgtctggata tcgaaggtca cagaatgctg
    15901 atttcgacca tctgatttca ttagtatctt gaaaatcggg taattacgac gctaaattaa
    15961 tgcaagaata aaaaacagag gaaccgtacg gaatgcgttt ctgccttatt ttgatcacag
    16021 cactgtttct ggccgggtgt agccaccata aagcaccgcc gccaaatgcc agactttctg
    16081 attcgattac cgttattgcc ggtttgaacg accagctaca aagctggcat ggcacgccgt
    16141 atcgttatgg tggcatgacg cggcgcggtg tggactgttc gggatttgtg gttgtaacga
    16201 tgcgcgatcg tttcgatttg cagctgcccc gagaaaccaa agaacaagcc tctatcggca
    16261 cgcaaattga taaagacgag ttgctgcctg gtgacctggt ctttttcaaa acgggttccg
    16321 gacaaaatgg tttgcatgta ggtatttacg ataccaacaa ccaatttatc cacgcctcta
    16381 ccagcaaggg agtgatgcgt tcctcacttg ataatgtcta ttggcagaaa aatttctggc
    16441 aggcgagacg aatttagtat gtcgaaaaat ggacggcgat gatacatcgc cgtccattta
    16501 ctcctgaata cagtagtatt ttgcagacaa aaattccgta aatggattat tcgtttataa
    16561 tcaggataat ctaaaaaaag gacccctgaa aaagtggtta acatggaaca aatgaaagtt
    16621 actccttatg taattgttat gtcttatggc ctcatccctt ttagccggat actgaaaaac
    16681 atccttcgag agggacggtt accatgaaga tttttttgga gaatctttat cattctgatt
    16741 gctatttcct gccgataaga gataaccagc aagaccttgt cggcgttgag ttaattaccc
    16801 atttctccag tgaagatggt acggttcgta tcccgacgag tcgggttata gcgcaactta
    16861 ccgaggagca acattggcaa ttattttcag agcagttgga attactaaaa tcatgccaac
    16921 atttttttat tcaacataaa ctctttgcct ggcttaattt aacgccacaa gtggctacat
    16981 tattattaga gagggataat tacgcaggcg aactattaaa atatccgttt attgaactgt
    17041 tgattaatga gaattacccg catctcaatg aaggaaaaga taatcgcgat cttttatcct
    17101 tatcgcaaat gtatcccctt gtgttaggca atttgggagc aggtaatagc acaatgaaag
    17161 cggtttttga tggtcttttc acccgcgtca tgctggataa aagttttatt cagcaacaaa
    17221 tcacgcatcg ctccttcgaa ccgtttattc gtgcgatcca ggcgcaaatt tccccttgtt
    17281 gcaattgcat catcgcgggg ggcattgata cgacagaaat attggcacag attaccccct
    17341 tcgatttcca tgctctccag ggatgcctgt ggcctgccgt tcctataaat cagataacga
    17401 cgttggttca gcgataaccc tcctgtttgc tggtgtttaa gacgagagta accgtctaca
    17461 ctattaaaca ggaggatcta tgaccctgtc ttttattacc cgctggcgcg atgaattgcc
    17521 agcaacctat acagcacttt cccctacgcc tttaaataat gcccggctga tttggcataa
    17581 taccgaactc gctaacacgc tgagtattcc atcgtcgctg tttaaaaatg gcgcaggtgt
    17641 ctggggcggc gaaaccctac tgcctggtat gtcaccactg gcgcaggttt acagtggtca
    17701 tcagttcggc gtctgggcgg gccaactggg tgatgggcgc ggtattttac tcggcgaaca
    17761 actgcttgct gatggcacta caatggactg gcatctgaaa ggtgctggcc tgacgcctta
    17821 ttcgcgaatg ggtgatggac gggcggtttt acgttcgacg atacgagaaa gtctcgccag
    17881 tgaggcgatg cattatctgg gcattccgac gacccgcgcg ttaagtatcg tcaccagcga
    17941 ttcgccagtg tatcgggaaa cggtggagcc aggcgcgatg ctgatgcgtg tggcaccaag
    18001 tcatctgcgc tttggtcatt tcgaacattt ttactatcgc cgcgagccgg aaaaggttcg
    18061 tcagttagct gactttgcca ttcgtcatta ctggtcatat cttgaagatg atgaggacaa
    18121 ataccgcctc tggtttagcg atgttgtcgc acgtaccgca tcgttaattg cccaatggca
    18181 gacggtcggc tttgctcatg gggtgatgaa taccgataac atgtcgctgc tggggctgac
    18241 gcttgattac gggccgtttg gttttctgga tgattacgaa cccggtttta tttgtaatca
    18301 ctcggatcat caagggcgtt atagctttga taatcaacct gctgtcgcgt tgtggaattt
    18361 acagcgtctg gcgcagacat tgtcaccatt tgttgccgta gatgccctga atgaggccct
    18421 ggacagctat cagcaggttt tgttgacgca ttatggacaa cggatgcggc agaaactggg
    18481 cttcatgacg gagcaaaaag aggataacgc gctactgaat gaattattca gtctgatggc
    18541 gcgagagcgc agcgattata cccgcacatt ccgcatgctg agtctgaccg agcagcacag
    18601 cgcggcgtca ccgctacgtg atgagtttat tgatcgtgcg gcatttgatg actggtttgc
    18661 ccgttatcgg gggcgtttgc aacaagacga ggttagcgat agtgagcgtc agcagctgat
    18721 gcaaagcgtt aacccagctc tggtgttgcg caactggttg gcgcaacggg cgattgaagc
    18781 ggcagaaaag ggtgatatga cggaattgca ccgcctgcat gaggcgttgc gaaatccttt
    18841 cagcgacaga gctgatgact atgtcagtcg tccacctgac tggggtaaac ggctggaagt
    18901 cagctgctcg agttaaaggc tacttggtca acagcagctt gccagcctga gttttacgga
    18961 gcagatattc ttgcccgtca tgatcgataa tcagtttgcc atccggacct aacagtgtct
    19021 ggctggaaat tcgccgaata ggctgcgggg aagcggtctt gtgattggca tccgtttcag
    19081 gcgtgagttt tctgctatcc gtataacgca taaaaattaa taataattga tataacaatg
    19141 attcttatta tcaatattat ggctgaccag agcaagcagt aatgtgctga gaagacaaca
    19201 agttgcgcag aaatgcccat cccatcagga atgggcacgc attcagaagc gggtatctac
    19261 cgcagaggca agtttttcga ccaggcgttc ggtatcttcc cagcccagac acgggtcggt
    19321 aatggattga ccgtaagtga gcggctgacc gccgacgatt ttttgcgttc cttcgcgcag
    19381 gaaactttcc gccataattc cagcaatcgc cgtagagcca ttgcggattt gctgacaaat
    19441 atcctcacaa acttctaact ggcgacggtg ctgcttctgg cagttaccgt ggctgaaatc
    19501 caccaccaga tgttcaggta aatcaaactc gtgcagcgta tcgcaggctg cggcgatatc
    19561 atcggcatga taattcggtt ttttgccgcc acgcataata atgtggccat acgggttgcc
    19621 gctggtctga tagatggtca tctgaccatt tttgtctggc gagagaaaca tatggctggc
    19681 gcgggctgcg cggatagcat ccacggcaat ccgcgagttg ccatcggtac catttttaaa
    19741 acctaccgga caggagagtg ccgaagccat ttcacggtgg atctgacttt cggtagtacg
    19801 tgcgccaatc gcgccccaac tgattaaatc agcaataaac tgaccggtca ccatatcgag
    19861 gaactcagtc gcggtaggga cacccagctc atttacctgt aaaagtaatt tgcgcgccat
    19921 ctccagaccg tgatttaccc gatagctgcc gtttaaatcc ggatcggaga ttagcccttt
    19981 ccagccgaca acagttcgtg gtttttcaaa ataggtgcgc attacgattt ccagccgtga
    20041 ctggtactgg ttgcgcagcg actgcagacg ggtggcgtac tccattgcag cggtgagatc
    20101 gtggatcgag caggggccaa taatgaccaa cagtcgctta tcttcaccat tcagtatttt
    20161 ttcaattctg cggcgggagt cggtgacatg ggtggcgacg ccaggcgtta cgggataccg
    20221 tagcgcgagt tcggcgggcg ttaccaggct ctcaatacgc gcagtacgga gttcgtcagt
    20281 tctgttcatt acaagtctca gaaatttgtt gcttctacgg caggactacc gggaagtgat
    20341 gcgcatcacg ataaaccaat cgcagctgat ttcaagtgcg aggtgtgtca cacctcgccg
    20401 ggtggttagc atgataacaa aaaaataagc taatgcacta gttctctagt acattcggcg
    20461 gctaagcccc atgatatcga ggagcttggt ggcaatctct tctaccgaat aattggtact
    20521 gttaatccac gggatctgat ttttacggta caacgcttct acttccgcga cttccatcct
    20581 gcactgacga agcgaggcat agcggctgtt ctcccgacgt tcctcgcgaa tcgccgccag
    20641 acgttccggg tcgatagtca ggccgaacaa tttatgctga agcggtttga gcgacgcggg
    20701 tagcaccaga ttgtccatat cgtcggcaat aaaggggtag tttgccgcgc ggataccaaa
    20761 ctgcattgcc agatacagac tggtgggggt tttaccacag cgagaaacac cgagcaggat
    20821 cacctgagcc tggtccagat tgcgcaacga aatgccgtca tcgtgggcga gggtgtaatc
    20881 aatcgccgca atgcgcgcat catatttatt gagattatta gggttaaggc catgggtacg
    20941 atgagcaatc ggcgttggat ccagtttcat ctcttgttgt agcggggcaa ccagcgcctg
    21001 aacaatatcc tggcaaaagc cttcactttg caagatgatg gcgcgaatct ccggcaacac
    21061 gatggagtag aagaccagcg ggcgcacacc tgtctggtga taaattgcgt caatctgatc
    21121 cttcactgcc cgtgcacggc tctcattttc gacaaacggc agcgtaatgc tgctgatagt
    21181 gacgggaaat tgtgacatta ctgcgtgtcc taatacctcc gcagttattg ccgtaccatc
    21241 agaaatataa aaaacgtggc gatcaacagc attatccatt ttgttcttcc cgtgatgcag
    21301 acataattct atgaaagcat aaattaaaaa cgcacagaag cgtaggacgt aatgtctggt
    21361 ttataaaatg aaccttcaat tttatttttt atgaaaacag catttcattt ttatggtttc
    21421 gtttataccg atggtttatg tggaaattgt cgaatagagc aggtttgcgc aacgctggga
    21481 tcagtcttaa aaagtaaaaa aatatatttg cttgaacgat tcaccgtttt tttcatccgg
    21541 ttaaatatgc aaagataaat gcgcagaaat gtgtttctca aaccgttcat ttatcacaaa
    21601 aggattgttc gatgtccaac aatggctcgt caccgctggt gctttggtat aaccaactcg
    21661 gcatgaatga tgtagacagg gttgggggca aaaatgcctc cctgggtgaa atgattacta
    21721 acctttccgg aatgggtgtt tccgttccga atggtttcgc cacaaccgcc gacgcgttta
    21781 accagtttct ggaccaaagc ggcgtaaacc agcgcattta tgaactgctg gataaaacgg
    21841 atattgacga tgttacccag cttgcgaaag cgggcgcgca aatccgccag tggattatcg
    21901 acactccctt ccagcctgag ctggaaaatg ccatccgcga agcttatgca cagctttccg
    21961 ccgatgacga aaacgcctct tttgcggtgc gctcctccgc tactgcagaa gatatgccgg
    22021 acgcttcctt tgccggtcag caggaaacct tcctcaacgt tcagggtttt gacgccgttc
    22081 tcgtggcagt gaaacatgta tttgcttctc tgtttaacga tcgcgccatc tcttatcgtg
    22141 tgcaccaggg ttacgatcac cgtggcgtgg cgctctccgc cggtgttcaa cggatggtgc
    22201 gctctgacct cgcatcttct ggcgtgatgt tctccattga taccgaatcc ggctttgacc
    22261 aggtggtgtt tatcacttcc gcatggggcc ttggtgaaat ggtcgtgcag ggcgcggtta
    22321 acccggatga gttttacgtc cataaaccga cacttgcagc gaatcgcccg gctatcgtgc
    22381 gtcgcaccat ggggtcgaaa aaaatccgca tggtttacgc gccgacccag gagcacggca
    22441 agcaggttaa aatcgaagac gtaccgcagg aacagcgtga catcttctcg ctgaccaacg
    22501 aagaagtgca ggaactggca aaacaggccg tacaaattga gaaacactac ggtcgcccga
    22561 tggatattga gtgggcgaaa gatggccaca ccggcaaact gttcattgtg caggcgcgtc
    22621 cggaaaccgt gcgctcacgc ggtcaggtca tggagcgtta tacgctgcat tcacagggta
    22681 agattatcgc cgaaggccgt gctatcggtc atcgcatcgg cgcgggtccg gtgaaagtca
    22741 tccatgacat cagcgaaatg aaccgcatcg aacctggcga cgtgctggtc actgacatga
    22801 ccgacccgga ctgggaaccg atcatgaaga aagcatctgc catcgtcacc aaccgtggcg
    22861 gtcgtacctg tcacgcggcg atcatcgctc gtgaactggg cattccggcg gtagtgggct
    22921 gtggtgatgc aacagaacgg atgaaagacg gtgagaacgt cactgtttct tgtgccgaag
    22981 gtgataccgg ttacgtctat gcggagttgc tggaatttag cgtgaaaagc tccagcgtag
    23041 aaacgatgcc ggatctgccg ttgaaagtga tgatgaacgt cggtaacccg gaccgtgctt
    23101 tcgacttcgc ctgcctgccg aacgaaggcg tgggccttgc gcgtctggaa tttatcatca
    23161 accgtatgat tggcgtccac ccacgcgcac tgcttgagtt tgacgatcag gaaccgcagt
    23221 tgcaaaacga aatccgcgag atgatgaaag gttttgattc tccgcgtgaa ttttacgttg
    23281 gtcgtctgac tgaagggatc gcgacgctgg gtgccgcgtt ttatccgaag cgcgtcattg
    23341 tccgtctctc tgattttaaa tcgaacgaat atgccaacct ggtcggtggt gagcgttacg
    23401 agccagatga agagaacccg atgctcggct tccgtggcgc gggccgctat gtttccgaca
    23461 gcttccgcga ctgcttcgcg ctggagtgtg aagcagtgaa acgagtgcgc aacgacatgg
    23521 ggctgaccaa cgttgagatc atgatcccgt tcgtgcgtac cgtagatcag gcgaaagcgg
    23581 tggttgaaga actggcgcgt caggggctga aacgtggcga gaacgggctg aaaatcatca
    23641 tgatgtgtga aattccgtcc aacgccttgc tggcagagca gttcctcgaa tatttcgacg
    23701 gcttctcaat tggctcaaac gatatgacac agctggcgct cggtctggat cgtgactccg
    23761 gcgtggtgtc tgaattgttc gatgagcgca acgatgcggt gaaagcattg ctgtcgatgg
    23821 ctatccgtgc cgcgaagaaa cagggcaaat atgtcgggat ttgcggtcag ggtccgtccg
    23881 accatgaaga ctttgccgca tggttgatgg aagaggggat cgatagcctg tctctgaacc
    23941 cggacaccgt ggtgcaaacc tggttaagcc tggctgaact gaagaaataa aataaatccc
    24001 cggcggcgtt cagtcgccgg gtttatgtga tccccgaaga taaaacttat tcaatctctt
    24061 cacagacatc ctgcgttaat cgccgcataa tatcttttct taacaaaaac ttttgtattt
    24121 tacctgaggc agtgcgcggt agtttttcga ttaccacgat atgttcagga tatttatatt
    24181 ttgcgacccg tttacggcta aaaaaagcca ccacctcttc cagcgataat gaatgatgcg
    24241 gcgctttcag cacgacataa gcgcatgatc gttcacctaa acgttcatcg ggcattgcaa
    24301 ccacacaggc atcgtgaatt ttaggatgct gcaataaaat atcttccact tcacggctgc
    24361 taatattttc gccgccgcgg acaataatat cttttttgcg tccggtaatt tttatatagc
    24421 cagcctcatc catacggcag agatcgccgc tgtaatacca gccttcttca tccagcgcac
    24481 gggcggttaa ttcaggttca tcaaaatatc ccataaacac attggggcca cgcgaggctt
    24541 cttcaccttc gcaacctggc ggtaaggtct tgcgtgcgtc atcgaccact ttaatctcta
    24601 cacctgcggc agcgtaacca tcggtgtgca taaagcgcga caaaggatca tcgagattca
    24661 ccaccgcatg cggcgaactt tctgtggaac cataaacact taataattta atgccgagct
    24721 gctggcattc acgcgccact tttttgggga ttgtggttcc gccgcaaaga aagaaacgca
    24781 gcgctgacag gtccgcgggt tgtttctcta gtacattcaa aagatcatag acaaacggcg
    24841 ttgcgcctag catacaggtg caacgctgct gctcaagcag cgcgagacac gcatcaggag
    24901 tgaaaatatc taacaacacg ctgcgagcgc caattaagaa tggtgccgtt acgccatgca
    24961 gaaagcccgt tgcgtggccg agtggcgcag gcatcataaa gacatcctgc caggtcagat
    25021 tcagccgcgc gcaataagcc cgctcactgg cgagaatatt gttatgcgtt agcatcacgc
    25081 cctttggcag accctcggtt ccggaggtaa acagcaccgc agctaattca tcgccgtggg
    25141 tcgttatcgc cgtggtcagt ggggtattgt cggcgataat ctgacttaat gagagggaag
    25201 aggtggcggg agccagtttg tccacgccga caatttgttg tagttgtgga agctgatttt
    25261 gcagcggcag gattaaatct accgggcgcg tttgtttaaa caacgtcggt gcaaagaaca
    25321 tttttgcctg acacttattg agcacccaca ccagttctgc ttcccgccag gaaggcaaca
    25381 gcggcacgga aaccgcaccg attttcaggc aggcaagata gataacggta aattcacacc
    25441 agccaggcag ttgaaatgcg atgcgatcgc ctgattcaat acccttcgct aacatccagt
    25501 ttgccagaca gctcgcggcg tgatcgagcg cgctataggt gtacgatgca ccatgattat
    25561 cgaccacggc aattttgtct ggcatcgcac gagcggtctg ctgccagtaa tcggccagcg
    25621 aagcatcgcc ccatagccct tgctgacgat acgccgcacg acgttgttcg ttgaacgtta
    25681 atgtcacttt catgttggac tctcgaaaca gaacatcagg cccgagatgc gggcctgtgg
    25741 gatgcatgat tagccgtagc gaaaatcaat accgaaggtg cctgcgggat attgccactg
    25801 ttcgagaata gtgttaccgc acagcacccg acaggtgccg cactccagac aaccggcaga
    25861 atcaaaatga atgttgccgg cgtcatcctg cttataaagt ccggcagggc aggctttcat
    25921 taatttacgg aattcattga tatcgggatt tgccgccaga atgatatgcg ggtggccctc
    25981 atcaacatgg aatttattga cgcctaattt gatgtcgacg ttaaccgtag cgttctggct
    26041 catagcgcgg ttgctccctt aatgccatct ttcagcaagt tgatcagccc aattttcttc
    26101 gcatgtccca tgatcatttt gcgtaccggc tggtttggtt tgccgtcaat ggtgaacatc
    26161 tcgttcatga tgtcggcgac cattcgtggg tattggctaa acaggcgcgg gttttccatc
    26221 agcgccggga tcttgcggaa atgctgcata tcgcgcatga tgcagctttg ttccagctca
    26281 cgtttgtatt gcgccagact gctggcggag aaatcctcgc gttctttggc ggcgatcacc
    26341 gttgtggcgg cagcctgagc cgatgcaatg gctaaatcca tgccacgaac tgtaaaaccc
    26401 aaattcaggc agaagcctgc ggcgtcacca acgatcatca cgccatcgtt aaccatctgc
    26461 ggcaccattg ccagaccgcc ttctggcacc atatgcgcgg aatattcaag cagtttgccg
    26521 ccgctaatta gtgggcgaat ggcggggtgt tgtttaaaat cttccagcat ttgcggcacg
    26581 cttttttgcg catgggcgat atcacccaat ccacaaacca gccccaagga tatggaatcc
    26641 ttgttggtat agagaaatcc cccgcccatc aggccgtcag aaggggaacc ggcaaacagc
    26701 caggcggcac cttcctcgcc cgtaatatta aagcgatcgt tgatctgttc tggtgtgagg
    26761 ccaataacct ctttaacacc aacagcgtaa tgatgcggat cggaagcggg aaccattccc
    26821 agcgagcggc caagcatcga gttaacgcca tcagccagaa tcaccacatt cgcttcgaga
    26881 atatcatccc cagcctgcac gccagtgacc ttgtttcctt cacgaaccag cgcatcgacg
    26941 cgaacgcctg ggataaactg cgcgcccgcc tgctcggctt gttccatcaa ccacgggtcc
    27001 agacgattac gcaatacggt ataagatgcg tgttgcggaa catctggttg ctcgcggtga
    27061 aaatcgaggg taacggcgct ttcttcggtt aagaaggaga ttttctcgcg tgtgaccttg
    27121 cgttctaccg gcgctgatac tgcaaagcct ggaatgattg cttcaagtgt gtgggcataa
    27181 agacgcccgc cggtcatgtt tttacatccg gcactgtcgc cgcgttctat caccaggaca
    27241 tccagcccgg ctcgcgccat gaccagtgcg gcaacgctac cagcaacgcc cgcaccgacc
    27301 acaatggcat caaatttgtc ttccgacatg cgttatgctc ccttcccgct gcgtttacag
    27361 cgggagatgt ggttgaaagg ggaattagcg gctcaactgg ctaatcaggg cagggacgac
    27421 tttgtagata tcgcccacca gaccgtagtc ggcataattg aagattggcg catttttatc
    27481 tttattgatg gcgacaatca cttttgcgcc gttgccgcca accatatgct ggatctgccc
    27541 ggagatcccc agcgtcaggt agagatcgga tttcagcaac acgccggaga caccgatata
    27601 acgttcacgc tccatccagt tctcgccttc ggcaattgga cgtgaacagc cgacttcagc
    27661 attcagcacc gccgccagtt cgtggaccat tttcaggtca tcctgcgccg ccagaccacg
    27721 accgacgcca accacacgtt ttgctttgct caggtccacg ctgcttgcgg ctttcgcacg
    27781 gcgttcgcga cagagaattt catgacgcgg agcaacataa ggtaccgttt ctgtcgggca
    27841 ctgatgagag gtatcactgg tgcacggttc ctgaacaccg ggtgcaaggg taatgatcgc
    27901 cagcgggctg ttgatctttt cctgagcgaa cgctaacccg ccatacatcc ggtgttcggc
    27961 gcaaatgtga ccatcgacaa tatccaccgc cgtggcatcg ttcaccagcg ccgcattcag
    28021 ttgcacactt aaccgtgctg ccagagcttt accacgtttc gtcgcggcca acagcaacat
    28081 agcggggtgt ttatctttca gcagggcagc aatgctttcg gcgtaatttt cagtgcgttg
    28141 cagcgcgtcg ttttgctcaa gaacataaat acattttgga ccataaggca taactgcctg
    28201 cgcctggtcg gtattttgta caatggcata cacctgttgg ccccattgct gagcgccgcc
    28261 aaacagttca gcataacgtt caggattatc gctaaagacc cagacgctgt ttaattgact
    28321 catcgtttct tctccgtagg cttcagttca gggctttctt cagatgttcc gccagctcgg
    28381 caatggcctc cggcgaatcg ttatcgataa tgatgtgctt acgttctgtt tgcggcggta
    28441 cgcgaatgcc gaccagttca gcaagtggcg cgctctggct ccagtcaata tcacttgcct
    28501 gccactgatt taccggtttt ttacccgcgc cgagaatggc tttcatcgaa ggaatgcgtg
    28561 gcacgttaat atcggaggtg acgcagagca cggctggaac agagagttca ataacttcaa
    28621 catcatcttc aagcgtgcgt tcaatcacca gtgtattgcc ctgacgctga atagcactca
    28681 ctgcattaat caccggaagt tgcagaattt ctccgaccag caagccaacc tgctgggcat
    28741 aaaggtcgcc ggaaccttca ccaaagatca gtaaatcgaa gccgatcttt tcaattgctg
    28801 ccgccagcgc ttttgcggta tcgagaggca gtgcatgttc aagttgcgca tcctgcacca
    28861 aatacaggct gtgcggcccg cgggatagca cgtctttgcg cactttcgag ttctgcaaca
    28921 atgagccgcc aacggtcagc gcggctatct catcgtcatc tgttgcgagc tggcttgcag
    28981 cttcaatggc attgagatcg aactggctga ttttggcgtc ggcattgtcg aaattcaggg
    29041 tgtattctgg agtgacaaca atgtcctgtt cttcaggcac cagcttaaag caggttatta
    29101 ttttcatgat ttctcctgtg agtcgcatac ggaaccatct ccgatggaag gtaaaaatgg
    29161 cctgaaaata gtgtgcgata gcggtgcggg gttttcgacg gagcaagctg ttttccttgc
    29221 caacaatctt gcaaaaaaaa tgcgatcgct aaaacaaaag tgcaatttgc ggcaatatag
    29281 ttattccatg accgtacaat cttgcattca gtgtaaataa tcttgtttag ctctcgctaa
    29341 acgcggttgt ataaccgcaa tacacgccgt attttatccg gcatatattg tttttgcttg
    29401 ctcatggaat agggaggttg tcatgtatca acgctgtttt gataacgcca gtgaaacgct
    29461 gtttgtcgcc ggtaaaacgc cacggctttc acgttttgca tttagcgatg atccaaaatg
    29521 ggagtccgga catcacgttc atgacaatga aaccgagttg atttacgtca agaaaggggt
    29581 tgcaaggttt accatcgatt cttcgttata tgtcgcacat gcggatgaca ttgtggtgat
    29641 agaacgcggc aggctacatg cggtggcctc tgacgttaac gatccggcaa cgacgtgtac
    29701 ctgtgcgctg tacggctttc agtttcaggg ggctgaggaa aatcagctac tgcaaccgca
    29761 ttcttgtccg gtaattgccg cggggcaggg aaaagaagtc ataaaaacct tatttaatga
    29821 gctaagtgtg attttgccgc aaagtaaaaa tagccaaaca tcttcgttat gggatgcatt
    29881 tgcctatacg ttagccattc tttactacga aaattttaaa aatgcttatc gttcggagca
    29941 ggggtatatt aaaaaa
//
LOCUS       DAZGLJ010000033        29675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000033 DAZGLJ010000000
VERSION     DAZGLJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.033"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(130..777)
                     /gene="gloC"
                     /locus_tag="ACHK49_001377"
     CDS             complement(130..777)
                     /gene="gloC"
                     /locus_tag="ACHK49_001377"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO9262050.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(804..1352)
                     /locus_tag="ACHK49_001378"
     CDS             complement(804..1352)
                     /locus_tag="ACHK49_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO9262051.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="ACHK49_001379"
     CDS             complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="ACHK49_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO9262052.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="ACHK49_001380"
     CDS             complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="ACHK49_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO9262053.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLANYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQITEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="ACHK49_001381"
     CDS             complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="ACHK49_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO9262054.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="ACHK49_001382"
     CDS             complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="ACHK49_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO9262055.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="ACHK49_001383"
     CDS             complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="ACHK49_001383"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO9262056.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            11026..11805
                     /gene="elyC"
                     /locus_tag="ACHK49_001384"
     CDS             11026..11805
                     /gene="elyC"
                     /locus_tag="ACHK49_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO9262057.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGDGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(11782..12675)
                     /locus_tag="ACHK49_001385"
     CDS             complement(11782..12675)
                     /locus_tag="ACHK49_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO9262058.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYLQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="ACHK49_001386"
     CDS             complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="ACHK49_001386"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO9262059.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="ACHK49_001387"
     CDS             complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="ACHK49_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO9262060.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(13806..15038)
                     /gene="ycaQ"
                     /locus_tag="ACHK49_001388"
     CDS             complement(13806..15038)
                     /gene="ycaQ"
                     /locus_tag="ACHK49_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO9262061.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="ACHK49_001389"
     CDS             complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="ACHK49_001389"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO9262062.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="ACHK49_001390"
     CDS             complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="ACHK49_001390"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO9262063.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(17843..20107)
                     /locus_tag="ACHK49_001391"
     CDS             complement(17843..20107)
                     /locus_tag="ACHK49_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO9262064.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVYLPGSWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="ACHK49_001392"
     CDS             complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="ACHK49_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO9262065.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="ACHK49_001393"
     CDS             complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="ACHK49_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO9262066.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="ACHK49_001394"
     CDS             complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="ACHK49_001394"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO9262067.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="ACHK49_001395"
     CDS             complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="ACHK49_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO9262068.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="ACHK49_001396"
     CDS             complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="ACHK49_001396"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO9262069.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(25685..26773)
                     /gene="serC"
                     /locus_tag="ACHK49_001397"
     CDS             complement(25685..26773)
                     /gene="serC"
                     /locus_tag="ACHK49_001397"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO9262070.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(26972..27664)
                     /locus_tag="ACHK49_001398"
     CDS             complement(26972..27664)
                     /locus_tag="ACHK49_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO9262071.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            27794..29554
                     /gene="ycaO"
                     /locus_tag="ACHK49_001399"
     CDS             27794..29554
                     /gene="ycaO"
                     /locus_tag="ACHK49_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO9262072.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         7214 a         8064 c         7194 g         7203 t
ORIGIN      
        1 tcccctgcaa tattttgaat ttgcacgatt ttgtaggccg gataaggcgc tcgtgccgca
       61 tccggcataa acaaagcgca ctttgccaga aatccaggcc cgcattaaaa gcgggctttt
      121 ttatgtgcct taccagacgg gcatttcgtc ttgcaggaag ggattatgca ggcgttcata
      181 accaagtgtg gataatggtc cgtgacccgg aataaatgtc acgtcatccc ccagtggcag
      241 caatttatct ttgattgaag aaatcagttg attatgatcg ccacgcggga agtcactgcg
      301 ccctactccg cctttgaaaa taacatcgcc agaaatcagc agctttgccc gatcatcaaa
      361 aaacacgaca tgacccggcg tatgcccagg gcaatgtaac acctgtaaag tcacattccc
      421 tatgctgatg gtatcgcctt cgttcagcca acgatctggc gtcagcggct ggcactcttc
      481 cagaccaaac atacgacttt gcgcaggcaa gccttgcagc cagaactcat cttctttttc
      541 cgggccaaaa accggcacgc cgtaatgttg cgccagttcc gccgctgcgc caacgtggtc
      601 cagatgacca tgcgtcagca ggatctgcat cagtgtcagg ccgctgtcat caacttcctg
      661 tttgattttt tccgcatcgc cgccaggatc gaccagtgcg gccagacggg tttgttcaca
      721 ccagattaat gaacagttct gggagaatgc ggtgaccgga ataatacgat agttcatact
      781 gcccctgttt cgttaagcaa ttactaccag tgccgtgctg gcccggtatc aatatgcaca
      841 aagttactac gtggataata tcctacacca cctgcgcgca tagataacgc ggctttgcga
      901 atattgctta acgcgatacc ttcaatatgg aaatccatcg cctggccttt agtgtgatag
      961 cttttcttcg ctactccacg gctgcgggcg cgtagttcat tgttggtatc aatagaacga
     1021 taaccggaaa tgagttgcac cggtttgcgc gtgcctaaca acccttgcag gcgatacaac
     1081 tggtcgaata atcctgggtc gatggacttt attttgttcg cgcggtaatc gcggaaaaaa
     1141 tggttaagtt ttgccaattc ttcctgaata tagcctctgc catcgaaaaa ctccgctttg
     1201 attgactctc cggtatgaag attattgagt gtcaaaatgc gcgggcgtgg ggtagagagt
     1261 gttgcaaacg caggggtcgg caggatggcg gcaccgagtg caacgccacc aagcgccagc
     1321 aatttgcggc gattagcgtc gaatttgtcc atgataatca agtctacagg tcaatgttat
     1381 cgtttatatg cacttctggc gcacaagagg caccttaacc gcccgtaaag cagacgtcaa
     1441 gatgactcaa ccccagcagt gacgggggct gcagagaatc gcccccaatc atgctaatta
     1501 ttacgacaac tgatttcccc gaactacttc atttacctga ttaattgttc cgctttcgat
     1561 acgatttgcg agctggatcg cgcaggcaga tcataattgt aaatatctgt acgatactgg
     1621 gtacgaccat ctgcaccaac aaaggccgtc aggtagtaga gattcaccgg aatcgactgc
     1681 cgaatattga cgtaacgtgt atcaccctgc ttcagcgcat cagaaatacg tttgtcattc
     1741 cagcctgcat cctgcaacag catattcgcc agatcggaag ctttattcac tcgtacacag
     1801 cctgagctca atgcgcgtgt atcacgcttg aacagattgt ggttcggcgt gtcatgcaaa
     1861 taaatggcct ctgaactcgg catattgaat ttatagcgcc ccagcgagtt ccgtgggcct
     1921 ggagcctgct ggaagcggaa cggtaaattc gaggccgtga ttgtagacca gtcaacctgc
     1981 catgggtcaa tcgcttctct gctgttccag ccgcgcatca ccgtatagcc atggctttcg
     2041 agatatcccg gatcgttgcg cacttttggc agaatatctt tgcgtgccag agttggaggt
     2101 acgttccacg gcgggtttac cactacgttg ttaagggcac tgctcatcat cggcgttttg
     2161 cgatcggggc gaccgacaat gactcgcgaa tccagcacct gattgccgtt ctgatagtag
     2221 accagcgaat aggccggaat gttaaccatg atcccggtag aaagctctgt tggcagcaag
     2281 cgcaatcgct ggatgttgag agccaacacg ccagcacgct gggcgggcgt tacgtttaac
     2341 cagtcacgtg ttgccgggcc aatagcacca tctgccccca atccttgcca tgcctgaaaa
     2401 cgtttaacgg cttccaccag ttcattatcg taggcggcac gaacggcagg cgcaggttta
     2461 ctacgagacg tcgtttgctt gtccatcggc ttagtttctg ctgtttcaac agtaacagcg
     2521 gatgggctga ctaccgcgtc agttggcgtg tcatcgccag gtagagtaat tttcggcccc
     2581 ccgtccaaca tgcctgtgcg ttgcaatatt tcgcgcaacg ccggtacgtc gttactccac
     2641 tgccctgggc gcaacgttgc tttgccggtc agttggggcc acggtttggt gtcactgagt
     2701 aaggccagta acgattcatg catcgccgca tattgcggat gctgcggtgc cagtcctgca
     2761 acaaacgtag gcaattgacc tttatccagc gccagctgcc attggttaat caccgagagc
     2821 ggcggcgttg caagtgcata aggtttactg ctatatagcc agcgagtgcc tttgaccgga
     2881 atatttgcaa tgaaatggag atagcccatc atcgcatcag agagcactac gtcgcgtgcc
     2941 atcccgttaa caccaggatc ggtcagtaac tctacccatt tattaaactg cggctggaaa
     3001 ccggcaatcg ccacctctgc cagctgttgc tggaaggctt taacagcatc acggttttcc
     3061 cacatgggtt gcatatcgcg tgcggcatac aacagttgaa gctggttaag ataaaccggc
     3121 ttgtaacctg cgggtaattg agattcgatt tgcgtgcggg ctttttctgc cgcaccttca
     3181 ggcaatggct ggatccccgc cattattgcc gtggcttgcg cctgcggcag tgcctcgccc
     3241 tgttcactga cagccaccgg gctgtcgcca gggattactt caggctcatc ggcctgcgca
     3301 ttgaacagtg gagcgaatgt tacggccagg cacaaactga ttgccgacag ccgacgacca
     3361 cacatcatat taagcaacat cccttgcccc ctgtttttat catactctga atagccaggc
     3421 tgttatttta cgacttgcct gtacaacgcc aatactcacg aaagtatgac gagcaagcct
     3481 gttactcatc aggcttatct tcagtatata aacgccgata cgtttttaca taacctaatg
     3541 taaagaagtt taaagaaaag cgcgccgtaa tgtgccactt ttttagtgcg gaaacggagt
     3601 tttcggaaaa agaaaaggcg gcattgctgc cgccttaatt ttaactcgcc tgagaaggcg
     3661 cttcgtcact tcctggaagc gtttcaggga gttgcggcgc aaatcctcgc aggccgacga
     3721 cgtgaacgtg ttcggtattc tggaagactt tacgcaccag tttataggtg gtgcctttct
     3781 ccgggctgat attttccggc gctgcgatga tgagctgcat ttgcaaacgc tcacacaatt
     3841 caaacagcgt ggcgatagaa cgagcatcca gtcgcgctgc ttcatcgagg aacagcaggc
     3901 ggcaaggaga gatatcttta ccgcgcaggc ggcgagattc atcttcccag ctttgtacca
     3961 ccatcaccag aatcgacata ccggtaccaa tcgcctcacc ggtcgacaat gcaccagact
     4021 ctgcgcgcaa ccaaccatcg gaaccacggt taacctcaac ttccatttcc agatagttgc
     4081 ggtaatccag cagttcttcg ccaatggtct gcggcgtgcg ctgtcccata tcaatctgcg
     4141 gattaagacg ctgatacaat ttcgccaacg cttccgagaa ggtcagacgg ttgctgttaa
     4201 acagatcctg atgctgctcg tgctgttcag agagcacatc cagcagcatg gcgtgcgttt
     4261 cacgcacgtt cacgttgagg cgcacgctgt tcacctgacc aaacgatacg ttctgcaacc
     4321 cctggttgag catacggata cggttctgct cgcgctgaat ggttttgcga atgatgttcg
     4381 ccacgctgcg ggaactgatc gccagtttct gttcacggga ggttaattct tcggtcagac
     4441 ggctaagttc gatctccatc tgttcgatag cttccaccgg atcatcggta cgaataatat
     4501 cctgacgaat acgttcacgc agatgctgat aaaccgccac gaagaactga attttacgct
     4561 ccggacgttt cggatcttcc gacatgcgca gcacgtcgcg cagatgttcg ttatccgcca
     4621 ccgccagacg cagcgcaccc aatgccttat ccgacataga gcgcaaatca tcagcggaga
     4681 gataagccag ctcacgacgg tgtaagcggc gctcaacgcc gttatctttc accatgcgca
     4741 tcaccgcaca ccagcccgct ttggcggtca ctacctgctc gcgcatctca aagtaatccc
     4801 gctccagctt gcgcagtttg cgggtcaggt tgtccatctc cgcttcgcag aaggtaagcg
     4861 ctttttccag ttgattgcgg cgtgaacggt tattgctcag ttgcgcatgc agctcgtcac
     4921 ggcgaatacg cgcccgctct tctgccccgc tatcagcacg cacgccgata tcctgcaatt
     4981 cacgttgcag atcgttgagt agctcttttt tggtgtcgta agaacttttc agcgaagcca
     5041 gcacctggtt gtactgactc aactgcgctg cgtgaccacg caacgcttca cgagcgcgag
     5101 tacgctccgc ttccgcctgt tccagacgtt cacgcagctt ttcgttgaga tcgctattac
     5161 cgctcagcat ttctgccgag tcagaatagc taaagtgcgc acgacgctgc accacttccg
     5221 tcagggcaaa cgcctgctga cgggcatcgc gctgcatctg ctgagagtac gcgtaatctt
     5281 cttttagctg ttcgaactgt tccgggtcgc tctgcaatac cgaaacgatc ggttccagtt
     5341 tcgccagttg attgccaaac tgctgaacaa aacgcgcggc ttcctgggct tcatccagac
     5401 gttcgcggat ttcatcgacg cgatccgcca ggctgtcatc agccaacagg ttgagacgcg
     5461 gcagaatgcg gttcagcgcc gtaacgcctt ctttcgcctg ctcaaactga atacgctgct
     5521 gctggttatc attttcatga ttactcaacg cccgctccag ttcgacgcga cggctgttca
     5581 gttgacggat ttctgcttcc gggtcagact caaacgcaac cgccagatga ctgccgataa
     5641 agcggctgaa cgcctgatgc agacgctgag ttttctgtac atcaaaggag agcgtggcga
     5701 agcgttcgga aagcacttca cgctcggcat ggaggctttc aatacggctt tcacgcgcag
     5761 cacgaccaaa cagcggcact tccgggaaac gtgaataacg ccactgacga tcggcgattt
     5821 tcaccactac cgctttttcc agctcatcaa cgctgaacac gctgtcatcg aatgactgcg
     5881 gatctccttc gatcagatag agatcttccg ggcaatcggt caagccttcc aggtgttcag
     5941 ttatctgtga cagatctggc accacgatgg cgtggcgtga cgggccatac agcgctgaga
     6001 agtacggcgc atcttccagg ctaacgtcgt cataaatttc tgacagcagc acaccaccaa
     6061 aacgctccgc cagcgcgttc agacgctgat cttcagagcc gccaggctgg cttaaacgtt
     6121 cgatctcttc atcgacggcg tttttgcgcg cgcccacttc atcgcgttca acaatcgcct
     6181 ctcgctcacg ctccagcaac tgttgcagat attcggtgac gtcctggctg gaggtaaact
     6241 cttcgccgca ctgttcgctc aactggttga gactgttttg cgctgccagc caaaccggcg
     6301 cacgctgcat caaactctga atgcgagact gcagctgttc ctgttcctgg cgcagtgcca
     6361 tgcgctcttc acgggcgtta gacacgctat cggaaagaga ggcaatgcgc gcttccagct
     6421 cctgatgcag agcttccagc tcgtcaatgt caaaattctt gccctgacgt ttgcagaaat
     6481 ctgccagcag acgctcagct tcttgctgtt cgcgcagacg ctgttccagt tcgcttaagc
     6541 gcatccgcaa cggctgaacc tgctctgcca ggtgacgctg atcgacccct tcgcgcaata
     6601 gttcgcgagc gacatcccac gcctcgttac gcgccagcgg gccgttgatt gccaccacca
     6661 gctgataagc ctgctcaaac tggctgtgcg cggtttgcgc catgctcatt ttctgctcaa
     6721 gagagagcat tttttcagtc gcttccagct ctttcgcctg gaaggtttcc agccattcgg
     6781 cggcgctgtc ggcggttaag tccggcagat ggcacagttc tttggcgcga ttaagcgcag
     6841 caatcgcctg gttatactgg atcgcgcgcg tttgctggac gtccagcgcc tgctggtagt
     6901 tagcaagctg gcttttcagc tcgtccactt ccagttcggc agcttccgcg cgagcctcgt
     6961 tctcttcctg gcgttcgatg gcttctgcca ccacttcgtt ttgctcttcc agacggatct
     7021 gcagctcatc gagatccgct tcgtagcgtt cgatcttctc ctgctgacgc agtgcggttt
     7081 gcaccaggtt caggtgatcg ctggccgcct gataatccgc ttccagatcg ccttcggcac
     7141 cgttgtgctc tgccagctca cgcgccatat cgacgtgttt gtactgctca gccgccagtt
     7201 gctgacgcga agtatgtaac tcgcgacgaa actccagggc tttgtcgaga tggacacggc
     7261 gctcgttggc gtgacgcatg tagtccgccg ccacgtagtt ggtagcttcg ctgatcagat
     7321 gcttaaacag gtcacggtcc gactgggtga cacgaatcgc ttccagcgtc atgcggtttt
     7381 cacgcagcgc cgcttccatg tcctggaacg ctttacgcac gccgctgttt tccggcaaca
     7441 ggtagtcgcg cagagaacgg gtaatggcac tggagatccc gccatacagc gaagcttcga
     7501 tcagacgata gaatttgcta cggtcagatg cggagcgcag acgacgcgcg atgatgccca
     7561 gatcgaacat cagcgagtgg taatcggtaa tggagttgaa ctgtttaaac tgcacgcctt
     7621 ccatcgcctc gagcttgtct ttcagctcgt taagcggcag cacgcgcgcc tggcgttcgt
     7681 tcagggtttc ggtcaccagc tgtgtcggct gcaccgacat cggcagtccc tgaatggcaa
     7741 atggtttgat atccacttta cgatcgcgtc cggcaacctg ttgcagacgc acaccgacca
     7801 ccacgcgctg gtggcgcgag ttaatggtgt cgagcatcga ataacagaca cccgctttca
     7861 gcttaccgtg cagaccttta tcgcgcgaac cgctggtggc cccggcttcc gtagtgttac
     7921 ggaaatgcag cagggtcagg tcggggatca gcgccgtaac gaacgccgcc atggtggtgg
     7981 atttacccgc cccgttaccg ccagaaagcg tcgtgaccag ctcgtcaagg tcaaaagttc
     8041 gggcaaaaaa gccgttccag ttaatcagcg tcagtgagcg aaatttaccg cgttcaatca
     8101 ttattcttcc tctccgctat ctggctgatt ttcttcggtt tcatcgttga gttgcagatg
     8161 attttcaatc ggcattgctt cgccatcacg aatcaggcga cgctgtgctt cacggggatc
     8221 gtcgccagca cgcacatcgg ctccgaagcg gaacaccgat tcggtaatgc gaaacttgct
     8281 gctgtcgtgg cccataaacc acaccatgcc taaacgacgc agacggttga gcgaagaacg
     8341 tactttctcc tgcaacttct gacggtcaac gtctgaaccg gttgaacggt tgttcaccag
     8401 tttcagcagt tttgcttcat cggccagggt gagcagttcg tcgtacagtt cctgctgggt
     8461 gaaaatcccc tcattcgcca gccgttccgg gctgagatag agataacaga ggattttccc
     8521 gaccatcata tccagttccg acaagacgga acgagggatc agcgtggtgg aacgtgggcg
     8581 taaatagaag aacccttctg gtgcgcgaat aagctcaacg ttataacgcg cgtaaaactc
     8641 ttccagatat tcctgaaaat ccatcaggaa tgcatgatta tccagttcgt cgaggccaat
     8701 atggcggcct gaacgtaagg cgctgtccag cgccggaaat aacggattcg ccagcgcctg
     8761 cgccagctta accggcatca cttgttcaat atttgtcgat gacatgcgcc tgtaccttgg
     8821 ctccgtaatc attaatcggc tgccatttcg ctggcagtcc ggtgaaatct gcttgcgcta
     8881 cgccaagacg taccgcctga tcaataacaa tacgcgcaac gtcaaagtga cgtgcacgcg
     8941 gatactgtga cagatattcg cgtaccacca gaccaagatc cagcggcact tgtctggttt
     9001 tgtacacggc aagttgttct tcgatgatcg ccgccagctg ttcgcggatc tcgttaaact
     9061 cttcgtattc cagatcctca ggaagttccc cagtcacttc ttcatcgcgc agtgccatct
     9121 cttcgtcacg catatccagc agacgatcgg cattggcata agttagcgcc cacggctcat
     9181 caaaataggt ttgtaccgac tgacgtaacc gctgagcaaa gacgcggttt ttatccatat
     9241 cgatcgcggt acgaataaat ttgtgtacgt ggcggtcgta gccaatccac aagtcgatgg
     9301 attgctggcc ccaactgata atacgatcga gtttgctctg cagatcgaac accagacgat
     9361 cgacgaaatg cagatcgtca tgggtcatcg tcgcatcctg aatgcgcaac agattagcct
     9421 gcaatttgtc gcctgccgct tccagcgtat cctgcaattc acgcagcgtc ccggaagttt
     9481 ccgaaagcaa caattcacag ctggaaatcg ccgcccgcca gtctttgttc agcaactggg
     9541 cgatatcgtc cttcacctgc tgctgctgtt cgtccatcag acgttgcgtc aggtcgatac
     9601 tgtcgaaaat ttctgctacc gaatatttca gtggcgcata gacattacgg tgccagtgaa
     9661 attcatcacc gccctcttcg gcggcatctg ctgcgcgttt gagctcaccc gccacaatcg
     9721 acaactgcat agaaagacgc agcgtagaaa actcgcgctg acggatgtag tagtcagtaa
     9781 tgccgatgcc gagcggcgtc agacggtaaa ttgcgttccc ttccgcctgc tcgctggtaa
     9841 agcggttcag cagacgttga cgcaccatgt cgttgatcgc gttattggcg cgcacgccga
     9901 tggtttcgct ggtttgctca aacgcatcac tcacatggcg gaatgcatcc accagctcgc
     9961 cttcactcat ctcaccatcc agacgctcgc cgttcagcgt ggcaaccgcc agcagaaaag
    10021 agagtcggtc taccggcagc gagatggaga agtcattttt tctggcccag gcaaccagtt
    10081 cggggactgt ctgggaaaat tcactcatac tttatccttg ctctgcggtt tgcgcgcggt
    10141 gacatgaata taacgcccca gggtaatata cggttcctga cggcaataac gcgtttctaa
    10201 ttcaagtaat gcttcatagc agtcgcgctg ctggtgcttc tcgcgcagat aatcatgaaa
    10261 cacgcgaacg cctgtcttac ccataatttg ccaaccagct tcttccagcc acagataaac
    10321 ctgcgccggg tcgcgtggat aatctggcga aagcgtccgt tttttctttt tcggcattcc
    10381 cgcctgcacg taatcaaaat tcccggcgac catgttatgc atcaacaaac catgcgcatt
    10441 gtagaacatt aacgacaaca cgccgcctgg acgcaacact gaccacaggg tctgcaatac
    10501 actgcgggga tcggccaccc actcgagcac cgcatggaac aatatcagat caacgggcgt
    10561 ttccaaatgc gaagcaacat cctgagcggc gcaatgtata aattgcatgt tgtcgctcac
    10621 accttttgct tctgccgcct gttttgcgcg gtcgatcatc tgcgcagaaa gatcgcataa
    10681 aatgacctga tgcccacgct cggccatttt gattgcggtc tgcccttctc caccgccagc
    10741 atccagcaca cgcagttttt gcgggcccat ttccgccagc acgcgatcga gatcctgcca
    10801 cagaatagcc tgtcgaagct gccctttggt ggtgccgtaa atgttacggg aaaacttttc
    10861 cgcaatatca tcaaaattgc gatcctgcat tactttctcc accggttggc taagacgttt
    10921 tctgataaag acgctatttt gtcacagcca cggcgagaat gaacccgtct ggtgtaatat
    10981 cgctggcgat cccttgctat atggttaaaa aaggaaccag aaaggatgct ttttacactg
    11041 aaaaaagtga ttggcaatat gttgctgcca cttccgctga tgttgttaat tattggcgct
    11101 ggcctggcgc tgctgtggtt tagccgtttt caaaaaaccg ggaagatttt tattagcatt
    11161 gggtggctgg cactcttgtt attgagtttg caaccggtag ccgaccgcct gctgcgtccc
    11221 atcgaaagca cctatccgac atggaataat tcgcaaaaag tggactatat cgtggtgctc
    11281 ggagatggtt atacctggaa cccgcagtgg gcaccgagct ctaatttaat caataacagt
    11341 ctgccacgcc tgaatgaagg tattcgctta tggcgcgaaa atccgggatc aaaactgatc
    11401 ttcacgggag gcgtagcaaa aaccaatacg gtgagtacag cggaagtagg tgccagagtt
    11461 gcgcaatcgc tgggcgtgcc gcgcgagcaa attatcaccc tggatttgcc aaaagatacc
    11521 gaagaagaag ctgcggcagt gaaacaggcg attggtgacg cccctttcct gctggtgacc
    11581 tccgcatcac atctgccgcg ggcgatgatc ttttttcagc aggaagggtt aaatccgcta
    11641 ccagccccgg cgaatcagtt ggccatcgac tcgccgctta acccgtggga acgggcaatc
    11701 ccttctccgg tctggctgat gcatagcgat cgtgttggct atgaaacgct tggccgcatc
    11761 tggcagtggc tgaaaggtcc gtcaggcgag ccacggcaag agtgattttg atgccagatc
    11821 gaagttgcgc cgactaaatc gtccggtatt aaccagttgc gcaacttcat cccataacac
    11881 atacaaccaa cgccgccaga tgaacgactc cgccactggc gcgcgttgca ggtaactcca
    11941 gagcaaatct tccgccagag aattatccat tagtcggaac agttcgtatt ctcgcggtgc
    12001 ccaaagcatt agtcccggcc cgaccatcgc cagtaactga tcgctgcgcg aatctttcag
    12061 catgctgcgt aaacagaagt taccgtgaat caacacacaa ttgtcgttaa agccttcaaa
    12121 taatgccggg agacattcgc gagtgcgaaa caggatccgc ttatcctgca tcgtcagacc
    12181 ggtgttattg aactgattga gcgtggtcca tagcacttca acatgttgca ggtaccatga
    12241 gggccagaaa ttttcctgag tattgtcgac cgcgccgacg caaccgcgac tgtcctgacg
    12301 gtgccaggcc agtaaggctt caacgatttg gtctttgagc tgttcccagc gttctggtgt
    12361 tcgggctggt gcctccaccg aaacaccacg catccgctcc agtagcagga catcagggcc
    12421 ggggtgttct tcatgcgtca tcacaccata aatagtcggc atacggacag tcccacttct
    12481 tgccagcatg gtggttttcc atgccagttg tcgggctttt ccgggcgtac taaagcttct
    12541 tgccattaac ggcattgggt ttccctggct gtcatacaaa gcccacaacg ccgtatccgc
    12601 tttttcattg acgcactcaa tacgactgag tttttcgccc agtaaatggc ttaattcggc
    12661 acgcagctgt tccattcaga ttaccctcat aaaagctact gctttcataa tgagcgccaa
    12721 tataaaagat gtcacctgaa tgagatcaat tgttgaaaag aaaaattgag gaaaatcgta
    12781 acggtacgac actcctccca aaattggctg aagtgtcgtg aagtgaaatt agcgcatttc
    12841 agcgcgaacg cgctcaagat cttcaggggt atccacacct gtgccaggaa cttcctgagc
    12901 aacagcaaca tggatttttt cgccgtacca cagaacacga agctgctcta acatttcgat
    12961 gtgttctaac ggacttgcct gccagttgac gtaacgacgg ataaagcctg cacggtagcc
    13021 ataaatacca agatgacgca ggaagttatc gccaacggtt tcaaggcctt ctgcaaaacg
    13081 atcacgatcc caaggaatgg tggcgcgtga gaagtacagt gcataccctt cagcgtcgag
    13141 aaccactttc accgcattcg ggttaaacgc ttcttccgca ttgtggattg gcaccgccag
    13201 agtcgccata cccacctgac gctgagcgag gttatcagca acctgacgaa tgattgtcgc
    13261 agggatcatc ggttcatcac cctgcacatt aacgatcacc gtgtcgtcgc tgaatgcgca
    13321 tttttcgaca acttccgcca gacgttctgt tcctgactga tgatcggcgc gcgtcataca
    13381 tacttcaccg ccagcggctt caacggcgcg ggcaacatcc tcatgatcgg ttgccacgat
    13441 gatgcgctcg gcacctgatt cacgcgcgcg ttcaagaaca tgaacaatca tgggtttgcc
    13501 gttaatatca accaatggtt taccgggcag acgcgtggac gcgtagcgcg cgggaataat
    13561 gaccacaaaa ctcatgattt actctcatca gcagtcagca cgcgggcttc cgtttccagc
    13621 aacactggaa tgccatcgcg gagcgggaaa gccaggttat ccagtttgca aataagctct
    13681 tgtttttcct ggttatacca aagttttccg ttgcaaaccg ggcaggcaat gatttcaagc
    13741 aaacgatgat ccatagttcc tccagatgga tcgggttatg aatgcataaa tcttatcata
    13801 atcatttatg cgacggggtc tatttcccag ccagtgcggc aatcagtaaa gagaccttgc
    13861 gggcagcgtc ctaatgtcac ccgcgttgcc tgctgccagc tcgcgaaatc agtaatcgcc
    13921 tgacgtaacc ctttttgcag cattgtcgtt ggtttaatgc cttcctgtaa ccacagagag
    13981 ataacttcaa ggatgcctgt ctggcgatgc attttggcat ccattcgccc aactaattgc
    14041 ccacgatgta ataatggcag aacaaaatag ccatactggc gtttcggcgc tggggtatag
    14101 cactccagcc ggtagctaaa atcaaaaagc tgctctgcgc gtttgcgatc ccagacaaca
    14161 ggatcaaaag gcgaaagtac cgcgctgtgc gttgcagtga gctttccggc cagcgcacgc
    14221 tcgagtagcg gcagcaaatc agcatgcagc caaagattgc ccaatttttc aacatgcaca
    14281 gcaatgattt gctgctgttc agccctcgct tcgcgccacg ccgccagtgc cggacgtttc
    14341 agccgatagt aatctgccag ccactgttcg cggaatattc ccaggctacg cgcactgtta
    14401 tccagcatga tgatttctgc ttctgtttgc gaaacgagat cgcgctcatc atcccagtca
    14461 ggcatgacac ggtgggttaa atcataaacg cgctggaagt tgcgccgttc aatcaccatc
    14521 acctttccgg cagtaaataa accttccaga tgccgtttat gcggcttcca ttcccaccag
    14581 ccgcttgcac ctttacgagg atgctcaaaa tcggctgaac gtaccggccc cttatcatga
    14641 atatgctgaa ttaactgtgc aatttccgcc tcatgttcct gcatccaggc gtctttgtat
    14701 ttccagccca ttttttcagg tgccagcatg cggtggcgaa taagacgaaa gtcgctacgc
    14761 ggcataaagc aggcttcatg cgcccagtat tccattaatt cgccacgcgc cagagactca
    14821 tccagccact gggcaggata atttcccaga cgactgaaaa gcaccagata tggactacgg
    14881 gcaacaatat tgatggtatc gatttgcagc aaggacatgc gggagatcgt tgccggaata
    14941 tcctccaacg acgcttgacg gcggggtttg tttaacaggc cttgtgcggc aaggtgaaga
    15001 ttacgcgcat cagcaaggga gaggtgcggc agcgacattc atgactccat caatcgaacg
    15061 ctgccgcggc gtaactagtt gccagaagcc agcgaggtta gttgcgtaag cagtttcgct
    15121 ggttcatcac ctgaaagctg tgcgtctaca ggcaaatacc accaattttc ttctgcaaag
    15181 gcccggcatt tcaccgcatc tttttcagtc attaccagcg tttgcccggc gcttaccaac
    15241 gcactgacat ccgcatggtt caaagactga tgatcggcca gcggtacaca tttttccggt
    15301 tgtacgccac acatcttcag cgtggcaaaa aagcgcggcg gatgcccaat ccccgccatc
    15361 gccactacat gttcaagctg agcaacgtca caacgcgtac cggtacgtaa attcaccgcc
    15421 tgacccggca gcagatgcat ggggatttca ccgctgcgag ggacaccgcc gttgacgatt
    15481 accgcatcaa ccgactttaa gcgccccgct cgctcacgca ttggccccgc cggcaaccac
    15541 cagccattgc caaagcgacg cacaccatca ataacgacaa tttccacatc acgcgccaga
    15601 cggtaatgct gtaaaccgtc gtcggttacg atgatctgca catcagggtg ttgcgccaga
    15661 atggctttta ccgcatcaga acgaacggga gaaaccgcaa caggcgcatc agtgcgttga
    15721 taaatcaaca caggttcatc acccgcctgt gctgttgtgg tatctgccga caataacagc
    15781 ggataagatt cagccttacc accatatccc cgcgatacga cccccacgcg aataccgcgc
    15841 tgttgcaact gttccaccag ccagacaacg accggggttt ttccgttgcc gcctgcggtg
    15901 agattaccaa ccacgacaac cggtacgggg gcacgccagg cgcgcttcag ttttagttta
    15961 tagcaaagac ggatcgcgcc acttaccagg ccatacaacc aggagagtgg cagcaatagc
    16021 cgccacaaag gggattcacc agaccagatt ttttcgatca ttggccaaac tgcattttgt
    16081 gaagttgcgc gtaaacgccg cggtgctcaa gcaaatcgtt atgcgtaccg cgttccacaa
    16141 tgacaccatc ctcgacgacc acgatttcgt ctgccttttc aatggtagac aagcggtggg
    16201 caatcaccag agaggtacgg tttttctgca actcatccag tgccgcctga atcgcacgtt
    16261 cggattcggt atccagagcc gaggtagctt catccagaat cagaatcggg ctatcacgca
    16321 gcaaggctcg agcgatagca atacgctgac gctgaccgcc agagagcagc acgccgtttt
    16381 caccaatcac tgtatcgaga ccgttatcca tcttattgat gaagtccatg gcgtaggcca
    16441 tacgcgccgc ttcttcaatt tgctcacggc tgtactgttc agtccgtgcg taagcaatgt
    16501 tgttagcaac cgtatcgtta aacagatgga cattctgcga caccagagca acctggttac
    16561 gtaacgacgc cagggtatac tcgcgcagat cgtgaccatc catcaggatt tcgccttcat
    16621 caatatcgta aaaacgagtg atcaggctgg cgatggttga tttacctgaa ccagagcgtc
    16681 caaccagagc aaccgtcttc cctgccggaa ttttcaggtt gatgttacgc aatgcaggta
    16741 cgtcacgtcc cggataagta aaggtgacat tgcggaattc cacgtcgcca gtcgcacgct
    16801 cgatcacgcg cttaccttcg tctttctcct gctcactgtc cagaatggta aacagcgtct
    16861 gacaagccgc cataccgcgc tggaactggg cgttaacgtt ggtcagtgat ttcagcggac
    16921 gcatcagtgc aatcattgaa gagaaaacaa cggtaatcgt accggcagtc aggctatcca
    16981 tgacacttgg gaagctcgcc gcatacagaa caaacgccag cgccaaagag gcgatcagct
    17041 gaatgatcgg atcagagatg gaagaggctg aaaccatttt catcccctga agacgcattc
    17101 ggttgctgac tttatcaaag cgtttcgttt ccacttcctg accaccgaaa atcaatactt
    17161 ctttgtggcc cttcagcatt tgttcggcgc tggtggtcac ctgccccatg gtgttctgca
    17221 tgtttttact gatgttgcga aaacgcttcg atactacgcg aatcgcaatc gaaacaatcg
    17281 gtgccagcac aatcaaaatg atcgacagtt gccaactgta atagaacatc atgatgaaca
    17341 ggccgatgat tgacgcacct tcacgcacaa ccgttatcag tgcgccggaa gaagaagaag
    17401 caacctgttc ggaatcgtag gtgatacgtg acaacagcgt ccccgttgac tgtttgtcaa
    17461 aaaatgaaac cggcattccc atcatgtgac caaacaggcg gcgacgcatg gtcattacca
    17521 cctttcctga tacccaggag atacagtagc tggagacata gctggtgata ccacgtaaaa
    17581 tcatcagccc gatcaccacc agcggcatcc acaccagcac ggagcgatct gttttaccaa
    17641 agccatcatc aagaagtggc ttaaggagcg ataacatgaa ggtatcgctg gctgcgttga
    17701 ggattaacgc tacgcccgcc acgatcagac ccgctttaaa aggcgcaatg gttggccaca
    17761 gtcggcggaa cgtctgccac gtagagagat ctttgtcgtt atgcattcaa aaaaccagca
    17821 tttgttgaaa tagccgcata ttctacccgt tatccactgg cacgccaaac cactgatgat
    17881 accaacgagg taaaatttgc tctctgaggc tgctaatccg ccagccttgc gctgaaaaat
    17941 tgaccgtcac ttgaccctga tgtggagtat caagccattg atatccttgc tgttgatagc
    18001 gatgcttaac tttattagag ggcaatcgcc atgcgttata gcgcgatgcc gatgcgagtg
    18061 ccacttttcc attcactcgc tgaattaatg gcaacgatga tgaggtatta ctgccatggt
    18121 gaggtacctg aagcaatgtt gtctgcactt gctgccagta acggcttagc atcttttgtt
    18181 cagcggggac ttcaatatca ccggttagaa gaatgctatt cgtcccgtca tcaaccttaa
    18241 ccacacagga atggttattt cctttatcgt tgctagcttg taaaggccag tgcacgctga
    18301 aacgcaatcc ttgccattgc cacgcttcgc cacgcacaca gggctgatga tgttcccagt
    18361 ttaacggact tctgatccat aacatcggcc atgtgtgcaa tattgagtcc agccctcccc
    18421 ggtgatccag atgttcatga ctcagaataa cgccttccgg ttcaagatta tgccagtgga
    18481 gccaggggat aatcagttgt tgcccactat ccccttcggg ccaggccagt cctgtgtcat
    18541 agagaatcgc tttgccgttt ctggctatca ccattgccag cccttgcccg acatcaagca
    18601 tgtacacctg ccactcgtca ggtcgaggtt tttgccacag cggccagcac atcagcaagc
    18661 ctacagccac acacattgct ggcaacgttc gccaggcgtt taatcgccat accaccagta
    18721 agaaccatgg ggaaaatgat agccattgcc aacgttcagc aatgttgatc cacccttctg
    18781 gcaaactctt taacccccag aaaagtaaag ccaaagaccg gtcggcaaga aaccataacc
    18841 cttgctccag gattaacggc ccgcttaaat gcacaaccat cgcggcgagg atcaacggaa
    18901 ccgtgataaa tgtcaccaag ggaattgcta atagatttgc aataaacgag gtcagactaa
    18961 tgccatgaaa tatgacgatt tgcacaggca taagcaggag tgtgattccc agttgcaaat
    19021 gaatgaggga aacaactgca cgcaataccg gcggcagttg ccactcagga cagggaaacc
    19081 actggtacca aaaaatcagt gccgcgaccg cagcggcaga gagccataaa ctttgcgaga
    19141 gaatggcaac aggatccatc agcaaaattg ccgccagaca acatatccat acatcccagc
    19201 cactccactg tcgcccactt aacttaagca ttccccacgt agcaagcgcc accacggtac
    19261 gcaatgcagg aggttgcatt cccgtcaacc aggcataaaa agcagcacag cagattccac
    19321 caattaatgg catttgccag tggatccagc tcccaggcag ataaacttgc ccaccgcgaa
    19381 tgagtccggc agccagcaac gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat
    19441 gcgccgttcc agtatcacgc atgatatttt tgatttcttt ggggacggat aaccgttccc
    19501 ccatacctaa accaagaata accgcattcc acatataggg ttgcagcgtt gtttgtagtg
    19561 acgccagata ctgtgcacgc aggctacaat tcggttcaat gacacttgcc tgcagaaagc
    19621 ggccggtgag cggctgatgc tgggcaatgg cataacgctg gctatcaaag ccgccatcat
    19681 taagttggcc atgaactgca cgaactttga gtttcattga ccattgttgt ccagcacaaa
    19741 ccgcttgcgg aagatattcg ccatacagca caaggcctgg cgcagggaat atacgtcgac
    19801 cttgtagatg agtaatttga ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa
    19861 caattgcatc ctgcgtcgcg ccagttaagg tttctcctgc ccacaaaatt tgctttgctg
    19921 ataatatgcc ccaaacgaaa aacagcaacg tcagcgcgat ataacggacg gttttaacag
    19981 ggataaatgc cagtacgcag gcgaagagag tcagaaacgc aagggttaat gtcccaggca
    20041 attggggcaa aatcagcaac ggaaaaattc cgcaaattat gcatacgccg actgtcgtta
    20101 ttttcatgca cccctccctg tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt
    20161 gggaattttg cgttccccga ggcacattca gaagaattta acgaaagggt gaaaactgct
    20221 caaaactctg cgaggcagct tccagttcct gattagagaa atgaaaaaag cacccgacag
    20281 gtgcttttct cgcgttcaag tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg
    20341 cagttcttta ccaggtttaa agtgaggaac gtattttcct tccagttcta ctttatcgcc
    20401 agtcttcgga ttacgtccgg tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc
    20461 gcggatttca atacgctcgc cctgcgcaag agtcgaggcc atatgctcca gcatctcttt
    20521 tactgcatct tcaaccgtct tggcgggaat gtgcgattgc tgggtggcaa gtctttctat
    20581 caattctgac ttggtcatga ttcctccggt tccttaaagg caaattaagc ggctgctgtt
    20641 gctgcattga ttaagggcgg ccgtagccgc ccttagtgct tgattacagg acgaaacctg
    20701 caatctgtca agtaaactca acaaacttcg gaataaaaat cccgaagagt cagagaatta
    20761 ctcgccttta gctgctttga aagcttcagc cattgcgttg ttggagaagt ttgcatcttc
    20821 ctgtttgtta acagttgcga ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga
    20881 caggctgatt gcgcggtttt tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc
    20941 aacgctcaga accagggtag cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag
    21001 gtaaccttca acgccgtcag ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac
    21061 tttaccggtt acgatagcgc ctttcttgtt cagagcaacc cagttgttga acggatcttc
    21121 tgcgagctgt ttaacgccca gggagatacg ttcacgttct gcgtcaacct gcagaacaac
    21181 tgcagcgatt tcgtcgcctt ttttgtattc acgaactgct tcttcgcctg caacgttcca
    21241 ggagatgtca gacaggtgaa ccaggccgtc gatgccgccg tccaggccga tgaagatacc
    21301 gaagtcagtg atagacttga ttttaccttc aacacggtcg cccttgttgt gggtttccgc
    21361 gaactgctgc cacgggttag ctttgcactg tttcagaccc agggagatac gacgacgttc
    21421 ttcgtcgata tccagaacca taacttccac tacatcgcca acgttaacaa ctttggacgg
    21481 gtggatgttt ttgttggtcc aatccatttc ggaaacgtgt accaggcctt caacgccttc
    21541 ttcgatttca acgaagcagc cgtagtcggt caggttggtc acgcgaccag tcagtttggt
    21601 accttccgga taacgtttag cgatagctac ccacggatct tcgcccaact gtttcaggcc
    21661 cagggataca cgggtacgtt cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc
    21721 cacgttgacg atttcgctcg gatgcttaac gcgtttccag gccatgtcag tgatgtgcag
    21781 caggccgtca acgccgccca gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat
    21841 acctttaact tccatgcctt cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt
    21901 ttcggattcg ataacggcac gacgagaaac aacaacgttg ttgcgcttct gatccagctt
    21961 gattacttta aattcaagct ctttgccttc caggtgcaga gtgtcacgca ccggacgaac
    22021 gtctaccaga gaacctggca ggaacgcacg aataccgttc agctcaacag tgaagccgcc
    22081 cttaactttg ccgttgataa caccggtaac agtttcagca tcttcgtaag ctttttccag
    22141 cgtgatccag gcttcgtgac gtttagcttt ctcacgggac agcagagttt caccgaagcc
    22201 gtcttctact gcgtccagag caacgtcaac ttcatcacct acctggattt ccagctcgcc
    22261 ctgggcgttt ttgaactgct cagccgggat ggcggactca gatttcagac cagcgtcaac
    22321 cagtactacg tctttgtcga tagcaacaac aacgccacga acgatagaac ccgggcgggt
    22381 ttcgatttct tttaaggact cttcaaagag ttgagcaaaa gattcagtca tgtttaatct
    22441 tcaggtttat atttaacgtc cacctggctc catgccggat ggggttgttt aacatacccg
    22501 ctgtcattcc attgcaacgg gggtactgca aattcggtcg cttatgcgag agccaatttc
    22561 tggcgcgcgt attgtagcgc tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc
    22621 aacactaaag catcggctgc cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg
    22681 tcgcgttctt tgatctcggc caaaaggcgc tcaaagttaa cactaaagcc cttctcctgc
    22741 aactgtagca tgcggcgatg cgcacgttct tccgaggagg cgtcaaggaa aattttcact
    22801 ggcgcatcag ggaataccac cgttcccatg tcgcggccat cggcaatcag gccgggtaat
    22861 tcgcgaaacg cgcgttggcg acgcaataat gcttcacgaa cgcgtgggaa tgccgcgact
    22921 tgtgaagctg cattcgccac ttcctgagta cgaatttcgc cgctgacatc ttccccttcg
    22981 aggatcactt ccagattgcc attggttgac acaaaacgta catccagatg ggatgccagc
    23041 ggtaccagcg catcttctga cgcaacatca acatggtgat gtaatgccgc caatgccagt
    23101 acgcgataaa ttgcacccga atccagcaga tgccattgca atgcttccgc catagcctta
    23161 cacaaggtgc ctttccctgc accgcttggg ccatcaatgg taataaccgg ggcaattgcc
    23221 gtcatcttta tctccttaaa acaggcgtac cgttaacata accgcgcgca ttatacgcgc
    23281 cgacgccaca aatcgttaac gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg
    23341 atttattgcg caattatagc gggctggcag aagaccagcc cgaaagatga caatgattta
    23401 cttaccagag gcgatacgat cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga
    23461 gtcaaacatg gactgggtgc gaccaccatc cagactagcc agtttttcaa agctgccaac
    23521 cagcccctgg gtgctgatgc cacgtttctt caacagatca aaggagaaat catctgcttc
    23581 ggactcttta tcacgggagt atttagcatt aatagcgcct tctgcgatat cacccaattg
    23641 tgagcgggaa agctgggaag ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac
    23701 gatcgcataa gaagctttca tttcagccag cgagtgaccc aacgcgacgt ggcccagttc
    23761 atggcccaga acaccttcaa tttcgttgtc attcatcatg tccatcaggc cactgtagac
    23821 acggacacag ccgttcgcca tcgcccatgc gttgacgtcg ctggtcatat aaaccttata
    23881 gttgaccggc gtgccattaa tattgttacc caatgctttg gcgatttttg ccagacgttt
    23941 accgtacttg ctggatttac ttgcgacttg attgccgctg tccatttcgg cacagccctg
    24001 attggcaatc gcttttgcat cggcatcgct taatgttgct gctttatagg catttaaacc
    24061 ggagctgata gccatattgg tatcaatacc gtgggtattt tgacaccctg tcagtaatgc
    24121 tgcagaggtc gcaatcgcca gcagtaattt agtattcttc atgtgaatca tccgttgatt
    24181 aatcatatat aaatattatg tatcaacaac ttacattgac aagcgacgct accattaatc
    24241 aaaatgactc acaaggtccg aaaaaaaacg ccgacaatgt gccgacgttt tgataaagaa
    24301 agatttggct atttattgcc cgttgttcat tcaggctgcc tggctaatcc gcgccaactg
    24361 ctcgaaataa tccggaaatg ttttggccgt acatttggga tcaagaatcg tcactggtgt
    24421 atctgacaac gccaccagcg agaaacacat cgccatccgg tgatcattgt acgtcgcgat
    24481 ctcggcaaag ttcagttttt ccggtggagt gatacgaatg taatcgtgcc cctcttctac
    24541 ttccgcaccg actttacgca gttctgttgc catcgcaaac aggcgatcgg tttctttaac
    24601 acgccagtta tagatattgc gcagcgtggt ggtgcctttt gcaaataacg ccgccgtggc
    24661 aatggtcatc gccgcatcgg gaatatggtt catatccata tcaatagcgt tcagttcacc
    24721 acgcgtgcag gaaatataat catcgcccca gcaaatggtc gcgcccattt tttccagcac
    24781 atcagcaaag cgaatatcac cctgcatact gttacgtcca ataccggtca cttttacagt
    24841 gccgcctttg attgctgctg ctgccagaaa gtaagaagcc gaagatgcat cgccttcgac
    24901 caaataagta cccggagact gataagactg cccgcctttt acgacaaatt gttgatagtg
    24961 ctgattttca atttcaacac caaacgtctt catcagattg agtgtgatgt cgatataagg
    25021 tttagaaacc agatcgcctt taatacgaat caccgtatct tccggcgcaa gaggcgcagt
    25081 cattaacagt gcggtgagga attggctgga aacggagcca tcaacgtcaa cgttgccgcc
    25141 agtaaagccg ccctgtaaac gcaacggcgg ataattttct tgttccaggt aagtgatctt
    25201 cgccccgccc aggcgcagcg catccaccag atgaccaatc gggcgttctt tcatacgcgg
    25261 ctcaccggtc agcacaatat cattgctacc cagacaaaga gctgccgcca gcggacgcat
    25321 tgccgttccg gcgttaccga ggaacaactc cagggcacct tctgcgtgta atggaccgcc
    25381 gttaccgata atttcgcaac gcgtacgatc ggctgaaagc gtatagctta cccctaacgc
    25441 tgttaatgca ttcagcatat ggcgcacgtc atcgctatcc agcagattgg ttaatactgt
    25501 tttgccgtgt gctaatgccg ccagcaataa agcgcggtta gaaacgctct tggaaccggg
    25561 cagattaata gtgccatcga cacgagcgat gggttgtaac gtcagggatt ccatgaactc
    25621 aactctctac aacagaaata aaaaccccac aggctggctg tggggattaa gcaaaatttc
    25681 ggcattaacc gtgacggcgt tcgaactcaa ccatgaagtc tgtcagcgct ttaacgcctt
    25741 ccagcggcat ggcgttataa atagaagcgc gcattccgcc gaccacacgg tgacctttca
    25801 gtgcatgaag gccagcagca aaagactctt caaggaacaa tttgtcaagc gcactgtccg
    25861 ccaactggaa cggcacgttc atccgcgaac ggttagcttt cgccacgtca ttgcggtaga
    25921 aatcgctgtt atcaatcacc ccatatagca gttctgcttt ttgctgattg attttatcca
    25981 tttcagctac accgccgttc gctttcagcc atttaaagac cagaccagat agataccagg
    26041 caaatgtcgg cggcgtgtta aacatggagc cgttatcgtt gaggatggaa taatccagaa
    26101 tcgacggaca cgcgatattc gctttgccca gcaaatcttc acgaacgatg acgattgtca
    26161 ggccagccgg gccgatattt ttctgcgcgc cagcgtaaat cacgccataa cggctgacgt
    26221 caatcggacg ggaaagaatg gttgaagaga agtcggcggc gaccaccaca tctttgccga
    26281 agtctggcgt ttcgtcgatg gcgataccgt cgatggtttc attcgggcag taatgcatat
    26341 aagcagcatt atcagagagt tgccattcac gcattggctt aaccgcgcgc agaccatcaa
    26401 cagtcacttt ggcgtcaaag acattaggcg tgcagtattt tttcgcttct ttaatggcac
    26461 ttgccgccca gtaaccggca tcaacataat ctgcggtggt tttatcaccg agaatattca
    26521 gcggtaccgc agcaaactga ccgcgaccac cgccatggca gaataatacc ttgtagttgg
    26581 aggggacatt aagaagatcg cgaaaatcct tctcggcttc ctctgcaacc tgaatgaact
    26641 ctttgccacg gtgactcact tccatcaccg acgtaccaag accgttccag tcgcgcagtt
    26701 cctgttgagc ctgtttaagc acctctgccg gtagcattgc cggaccagaa ctaaaattga
    26761 agatttgagc catttcccct caccacgttg cgttgctatt aacccgctgt caatcgattg
    26821 cgaccacagg taatacatta ttcctgtggc tatcggtttt atcattcagt gacacacatc
    26881 gcaatgagta aaaccgttga gggcaaaaat gtggcttgca tcacacaaaa gaatctctcg
    26941 gattattagc aaaaagccga tgtttttact gtcaggtcac ccgttttgcc cgacttgcct
    27001 tcgtccattc tggatttgca cagcgcggac ataattgttt ttcccccgct ttcatatgaa
    27061 tgatttcact acaacgaata caggcatagt ccgccgactc cggcaccact ttgtaacgct
    27121 gcgtacaatc actgggtaaa ataagtaaac ccggtttcac cttgtcatct tcaaagaaat
    27181 agacactgat ttgcccgttg gtttcgagaa tcgccagacg tacctgcccc agctgctcca
    27241 cgccacgcaa tcgtagctcc ataaagaact caaattccgt catgttggag ttattgagtt
    27301 tcgaccaggc cagctcgcca tcttcaataa taacaaccgg cttaccttcc agtagatctt
    27361 ccagtttttc actgtgcgcc atcaaccaca ttaccaggcg gtataacaac gccagagtga
    27421 taaagacgat aagtaccggc accatcggca catcatcata aaacgccaca tctcccgccg
    27481 ctgaacccag cgtgagaatg attaacactt caaacagcga catctgtcgc acaccgcgtc
    27541 ttccggtcat tttgaggaac aaaaaaacca gtacaaaggt ataaagacta cgtagtgcaa
    27601 cttcgccaag gaaatcaaaa ggcactttat caaatgccat acggtggaga tcgaacgcct
    27661 tcatttttat gctcttatcg tggtttatcc gctaaaagca tagtcaacag ttcatcggca
    27721 tggcggcggt gatagcgcct ttgagataaa acccgtatta ttgcgcgctt tccgtacgac
    27781 taaagtgatt ttcatgacgc aaacatttat ccccggcaaa gatgccgctc tggaagattc
    27841 catcgctcgc ttccagcaaa aactttcaga cctcggcttt cagattgaag aggcctcctg
    27901 gctgaatcct gtgcctaacg tctggtctgt acatattcgc gacaaagagt gcgcactgtg
    27961 ttttaccaac ggaaaaggcg caaccaaaaa agcggcgctg gcttctgcac ttggtgaata
    28021 tttcgagcgt ctctcaacca actacttttt tgctgatttc tggctgggcg aaaccatcgc
    28081 caacggtccg ttcgtgcatt atcccaacga aaaatggttc ccactgaccg aaaatgacga
    28141 tgtgccagaa ggactactcg atgaccgtct gcgcgcgttt tatgatccgg agaatgaact
    28201 gaccggcagc atgctgattg acctacaatc cggtaacgaa gatcgtggta tttgcggcct
    28261 gccgtttacg cgtcagtccg acaatcagac cgtttatatt ccgatgaata tcattggtaa
    28321 cctgtacgtc tccaacggta tgtccgcagg taatacccgc aacgaagcac gcgttcaggg
    28381 attgtctgaa gttttcgaac gctacgtgaa aaaccgcatt attgctgaaa gcatcagcct
    28441 gccagagatc ccggcagacg tgctggcgcg ttacccagca gtagttgaag ccatcgaaac
    28501 gctggaagcg gagggtttcc caatcttcgc ttatgacggt tcgcttggcg gccagtatcc
    28561 ggtgatttgc gtggtactgt tcaatcctgc taacggtacc tgctttgcct ctttcggtgc
    28621 gcatcctgat tttggcgtag cactggaacg taccgtgacc gagctgctgc aaggtcgtgg
    28681 cctgaaagat ttggatgtgt ttactccgcc aaccttcgat gatgaagaag tcgctgaaca
    28741 taccaacctc gaaacgcact ttatcgattc cagcggttta atctcctggg acctgttcaa
    28801 gcaggatgcc gattatccgt ttgtggactg gaatttctcc ggcaccacgg aagaagagtt
    28861 tgctacgctg atggctatct tcaacaaaga agataaagaa gtttatattg ccgattacga
    28921 gcatctgggc gtttatgctt gccgtattat cgtgcctggc atgtccgata tttatccggc
    28981 tgaagatctg tggctcgcga ataacagtat gggcagccat ttacgtgaaa cgattctttc
    29041 gctaccaggc agcgagtggg aaaaagaaga ttacctgaac ctcatcgagc aactggatga
    29101 agaaggtttt gatgacttta cccgcgtgcg tgagctgttg ggtctggcga ccgggtcgga
    29161 taacggttgg tacaccctgc gtatcggtga attaaaagcc atgctggcgc tggctggtgg
    29221 cgatctggaa caggctctgg tctggaccga atggacgatg gagtttaact catcagtatt
    29281 tagtccggaa cgcgccaact attatcgctg cctgcaaacg ttgttattac tggcacagga
    29341 agaagatcgc cagccgctgc aatatctgaa tgcgtttgtt cgcatgtacg gcgcagatgc
    29401 cgtagaagcc gccagtgcgg caatgagcgg cgaagcggcg ttttacggcc tgcaaccggt
    29461 agatagcgat ctgcacgcgt ttgctgcaca tcagtcgttg ttgaaggcct acgaaaagct
    29521 gcagcgcgcc aaagcagcat tctgggcaaa ataaaatcaa atagcctacg caatgtaggc
    29581 ttaatgatta gtctgagtta tattacgggg cgttttttta atgccccgct ttacatatat
    29641 ttgcattaat aaaataattg taattataag gttaa
//
LOCUS       DAZGLJ010000034        29325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000034 DAZGLJ010000000
VERSION     DAZGLJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.034"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            235..1122
                     /gene="rhtA"
                     /locus_tag="ACHK49_001400"
     CDS             235..1122
                     /gene="rhtA"
                     /locus_tag="ACHK49_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO9262073.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            1421..1924
                     /gene="dps"
                     /locus_tag="ACHK49_001401"
                     /gene_synonym="pexB"
     CDS             1421..1924
                     /gene="dps"
                     /locus_tag="ACHK49_001401"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO9262074.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            2328..3074
                     /gene="glnH"
                     /locus_tag="ACHK49_001402"
     CDS             2328..3074
                     /gene="glnH"
                     /locus_tag="ACHK49_001402"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO9262075.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            3213..3872
                     /gene="glnP"
                     /locus_tag="ACHK49_001403"
     CDS             3213..3872
                     /gene="glnP"
                     /locus_tag="ACHK49_001403"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO9262076.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVLYLIITLVLSFILRRLERRMKI
                     L"
     gene            3869..4591
                     /gene="glnQ"
                     /locus_tag="ACHK49_001404"
     CDS             3869..4591
                     /gene="glnQ"
                     /locus_tag="ACHK49_001404"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO9262077.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            4708..6933
                     /gene="ybiO"
                     /locus_tag="ACHK49_001405"
     CDS             4708..6933
                     /gene="ybiO"
                     /locus_tag="ACHK49_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO9262078.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPPPPGEPT
                     L"
     gene            complement(6930..7856)
                     /gene="rlmF"
                     /locus_tag="ACHK49_001406"
     CDS             complement(6930..7856)
                     /gene="rlmF"
                     /locus_tag="ACHK49_001406"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO9262079.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            8132..8377
                     /gene="mcbA"
                     /locus_tag="ACHK49_001407"
                     /pseudo
     CDS             8132..8377
                     /gene="mcbA"
                     /locus_tag="ACHK49_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
     gene            8655..10937
                     /gene="fiu"
                     /locus_tag="ACHK49_001408"
     CDS             8655..10937
                     /gene="fiu"
                     /locus_tag="ACHK49_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO9262080.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            10979..11656
                     /gene="ybiX"
                     /locus_tag="ACHK49_001409"
     CDS             10979..11656
                     /gene="ybiX"
                     /locus_tag="ACHK49_001409"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO9262081.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            11730..11996
                     /gene="ybiI"
                     /locus_tag="ACHK49_001410"
     CDS             11730..11996
                     /gene="ybiI"
                     /locus_tag="ACHK49_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO9262082.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKGLQKPAYTGYSRRGSKDSQLR"
     gene            12261..12521
                     /gene="ybiJ"
                     /locus_tag="ACHK49_001411"
     CDS             12261..12521
                     /gene="ybiJ"
                     /locus_tag="ACHK49_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO9262083.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(12753..13235)
                     /gene="ybiA"
                     /locus_tag="ACHK49_001412"
     CDS             complement(12753..13235)
                     /gene="ybiA"
                     /locus_tag="ACHK49_001412"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFO9262084.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            13355..15505
                     /gene="dinG"
                     /locus_tag="ACHK49_001413"
     CDS             13355..15505
                     /gene="dinG"
                     /locus_tag="ACHK49_001413"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO9262085.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            15533..16495
                     /gene="ybiB"
                     /locus_tag="ACHK49_001414"
     CDS             15533..16495
                     /gene="ybiB"
                     /locus_tag="ACHK49_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO9262086.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNTAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            16636..17721
                     /gene="hcxB"
                     /locus_tag="ACHK49_001415"
     CDS             16636..17721
                     /gene="hcxB"
                     /locus_tag="ACHK49_001415"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO9262087.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKESGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHKHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGALLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            17788..17847
                     /gene="ybiE"
                     /locus_tag="ACHK49_001416"
     CDS             17788..17847
                     /gene="ybiE"
                     /locus_tag="ACHK49_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO9262088.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(17952..19316)
                     /gene="rhlE"
                     /locus_tag="ACHK49_001417"
     CDS             complement(17952..19316)
                     /gene="rhlE"
                     /locus_tag="ACHK49_001417"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO9262089.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGDGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            19545..20216
                     /gene="cecR"
                     /locus_tag="ACHK49_001418"
     CDS             19545..20216
                     /gene="cecR"
                     /locus_tag="ACHK49_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO9262090.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            20219..21214
                     /gene="hlyD"
                     /locus_tag="ACHK49_001419"
     CDS             20219..21214
                     /gene="hlyD"
                     /locus_tag="ACHK49_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO9262091.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            21207..22943
                     /gene="ybhF"
                     /locus_tag="ACHK49_001420"
     CDS             21207..22943
                     /gene="ybhF"
                     /locus_tag="ACHK49_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO9262092.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            22936..24069
                     /gene="ybhS"
                     /locus_tag="ACHK49_001421"
     CDS             22936..24069
                     /gene="ybhS"
                     /locus_tag="ACHK49_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9262093.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            24080..25186
                     /gene="ybhR"
                     /locus_tag="ACHK49_001422"
     CDS             24080..25186
                     /gene="ybhR"
                     /locus_tag="ACHK49_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9262094.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(25148..25558)
                     /gene="ybhQ"
                     /locus_tag="ACHK49_001423"
     CDS             complement(25148..25558)
                     /gene="ybhQ"
                     /locus_tag="ACHK49_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO9262095.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            25691..26452
                     /gene="ybhP"
                     /locus_tag="ACHK49_001424"
     CDS             25691..26452
                     /gene="ybhP"
                     /locus_tag="ACHK49_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO9262096.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPKGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            26449..27690
                     /gene="clsB"
                     /locus_tag="ACHK49_001425"
     CDS             26449..27690
                     /gene="clsB"
                     /locus_tag="ACHK49_001425"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO9262097.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            27690..28646
                     /gene="ybhN"
                     /locus_tag="ACHK49_001426"
     CDS             27690..28646
                     /gene="ybhN"
                     /locus_tag="ACHK49_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO9262098.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPVGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(28682..>29325)
                     /locus_tag="ACHK49_001427"
     CDS             complement(28682..>29325)
                     /locus_tag="ACHK49_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO9262099.1"
                     /translation="LITALGLVATAGLAWGAKYLEITATKYDSPTMYVAIGLLLVCMY
                     GLSKDINKINAVIAGVIYLFLLSLVAIVVASLAPVPAIIVVFSTAGAMFLISMLAGLL
                     FNVDPGSHRFIIMMTLTGLALVIIMNAALMSERPVWVISCLMIVLWSGIISHGRNKLL
                     ELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFGILAAIAITLVWQRHTRFFH"
BASE COUNT         6951 a         7624 c         7766 g         6984 t
ORIGIN      
        1 acaccgctat ccatctaaat ttaaatcact ttttcagaga actgcgtaag tattacgcat
       61 gttttccctg tcattcatcc agattattcc taatcaccag actaatgatt ccatcaatcc
      121 tggcgcattt tagtcaaaac gggggaaaat tttttcaaca aatgctcaac catcattggg
      181 tatatccagt acactccacg ctttacttaa gtctagatat ttgtgggaga aaggatgcct
      241 ggttcattac gtaaaatgcc ggtctggtta ccaataatca tattgctcgt tgccatggcg
      301 tctattcagg gtggagcctc gttagctaag tcactttttc ctctggtggg cgcaccgggt
      361 gtcactgcgc tgcgtctggc attaggaacg ctgatcctca tcgcgttctt taagccatgg
      421 cgactgcgct ttgccaaaga gcaacggtta ccgctgttgt tttacggcgt ttcgctgggt
      481 gggatgaatt atctttttta tctttctatt cagacagtac cgctgggtat tgcggtggcg
      541 ctggagttca ccggaccact ggcggtggcg ctgttctctt ctcgtcgccc ggtagatttc
      601 gtctgggttg tgctggcggt tcttggtctg tggttcctgc taccgctggg gcaagacgtt
      661 tcccatgtcg atttaaccgg ctgtgcgctg gcactggggg ccggggcttg ttgggctatt
      721 tacattttaa gtgggcaacg cgcaggagcg gaacatggcc ctgcgacggt ggcaattggt
      781 tcgttgattg cagcgttaat tttcgtgcca attggagcgc ttcaggctgg tgaagcactc
      841 tggcactggt cggttattcc attgggtctg gctgtcgcta ttctctcgac cgctctgcct
      901 tattcgctgg aaatgattgc cctcacccgt ttgccaacac ggacatttgg tacgctgatg
      961 agcatggaac cggcgctggc tgccgtttcc gggatgattt tcctcggaga aacactgaca
     1021 cccatacagc tgctggcgct cggcgctatc atcgccgctt caatggggtc tacgctgaca
     1081 gtacgcaaag agagcaaaat aaaagaatta gacattaatt aaatttacat ttctgcatgg
     1141 ttatgcataa ccatgcagaa tttctcgcta cttttcctct acaccgtctt tatatatcga
     1201 attatgcaaa agcatattta ttccgaaaat tcctggcgag cagataaata agaattgttc
     1261 ttatcaatat atctaactca ttgaatcttt attagttttg tttttcacgc ttgttaccac
     1321 tattagtgtg ataggaacag ccagaatagc ggaacacata gccggtgcta tacttagtct
     1381 cgttaattac tgggacataa catcaagagg atatgaaatt atgagtaccg ctaaattagt
     1441 taaatcaaaa gcgaccaatc tgctttatac ccgcaacgat gtctccgaca gcgagaaaaa
     1501 agcaacagta gagttgctga atcgccaggt tatccagttt attgatcttt ctttgattac
     1561 caaacaagcg cactggaaca tgcgcggcgc taacttcatt gccgtacatg aaatgctgga
     1621 tggcttccgc accgcactga tcgatcatct ggataccatg gcagaacgtg cagtgcagct
     1681 gggcggtgta gctctgggga ccactcaagt tatcaacagc aaaaccccgc tgaaaagtta
     1741 cccgctggac atccacaacg ttcaggatca cctgaaagaa ctggctgacc gttacgcaat
     1801 cgtcgctaat gacgtacgca aagcgattgg cgaagcgaaa gatgacgaca ccgcagatat
     1861 cctgaccgcc gcgtctcgcg acctggataa attcctgtgg tttatcgagt ctaacatcga
     1921 ataaatccat cgctgatggt gcagaacttt agtacccgat aaaagcggct tcctgacagg
     1981 aggccgtttt gttttgcagc ccacctcaac gcacttattt agtgcatcca tctgctatct
     2041 ccagctgatt aagtaaattt tttgtatcca catcatcaca caatcgttac ataaagattg
     2101 ttttttcatc aggttttacg ctaaataatc actgtgttga gtgcacaatt ttagcgcacc
     2161 agattggtgc cccagaatgg tgcatcttca gggtattgcc ctataaatcg tgcatcacgt
     2221 ttttgccgca tctcgaaaaa tcaatgagtt gcaaaactgg cacgattttt tcatatatgt
     2281 gaatgtcacg caggggatcg tcccgtggat agaaaaaagg aaatgctatg aagtctgtat
     2341 taaaagtttc actggctgca ctgaccctgg cttttgcggt ttcttctcat gccgcggata
     2401 aaaaattagt tgtcgcgacg gataccgcct tcgttccgtt tgaatttaaa cagggcgata
     2461 aatatgtggg ctttgacgtt gatctgtggg ctgccatcgc taaagagctg aagctggatt
     2521 acgaactgaa gccgatggat ttcagtggga tcattccggc actgcaaacc aaaaacgtcg
     2581 atctggcgct ggcgggcatt accatcaccg acgagcgtaa aaaagcgatc gatttctctg
     2641 acggctacta caaaagcggc ctgttagtga tggtgaaagc taacaataac gatgtgaaaa
     2701 gcgtgaaaga tctcgacggg aaagtggttg ctgtgaagag cggtactggc tccgttgatt
     2761 acgcgaaagc aaacatcaaa actaaagatc tgcgtcagtt cccgaacatc gataacgcct
     2821 atatggaact gggcaccaac cgcgcagacg ccgttctgca cgatacgcca aacattctgt
     2881 acttcatcaa aaccgccggt aacggtcagt tcaaagcggt aggtgactct ctggaagcgc
     2941 agcaatacgg tattgcgttc ccgaaaggta gcgacgagct gcgtgacaaa gtcaacggcg
     3001 cgttgaaaac cctgcgcgag aacggaactt acaacgaaat ctacaaaaaa tggttcggta
     3061 ctgaaccgaa ataataacgc tacacctgta aaacgcactg gcagttccct ctcccctctg
     3121 gggagaggat tagggtgagg ggcgcaaacc cgctccgggg ccattaatta ccctgaattt
     3181 gattatttac accacggtaa caggaacaac atatgcagtt tgactggagt gccatctggc
     3241 ctgccattcc gcttctgatt gaaggtgcca aaatgaccct gtggatttcg gtcctcggtc
     3301 tggcaggcgg tctggtaatc ggattgctgg caggttttgc acgcaccttc ggtggttgga
     3361 tagccaacca cgtcgcgctg gtctttattg aagtgatccg cggcacacct atcgtcgtcc
     3421 aggtgatgtt tatctatttc gccctgccga tggcgtttaa cgacttacgc atcgacccat
     3481 ttactgcggc ggtggtcacc atcatgatca actccggcgc gtacattgcg gaaatcacgc
     3541 gtggtgcggt gctgtctatc cacaaaggtt ttcgtgaagc aggactggcg ctcggtcttt
     3601 cacgttggga aaccattcgc tacgtcattt taccgctggc actgcgtcgt atgctgccgc
     3661 cgctgggtaa ccagtggatc atcagcatta aagacacctc gctgtttatt gtgatcggcg
     3721 tggcggaact gacccgtcag gggcaagaaa ttattgccgg taacttccgc gcccttgaga
     3781 tctggagcgc cgtggcggtg ctctatctga ttattaccct ggtgctgagc tttattctgc
     3841 gtcgtctgga aagaaggatg aaaatcctgt gattgaattt aaaaacgtct ccaagcactt
     3901 tggcccaacc caggtgctgc ataatatcga tttgaacatt gcccagggcg aagtcgtggt
     3961 gattatcggg ccgtccggtt ccggtaaatc gaccctgctg cgctgcatca acaaactgga
     4021 agaaatcacc tccggcgatc tgattgtcga tggcctgaag gttaacgatc cgaaagttga
     4081 cgagcgcctg attcgccagg aagcaggtat ggtgttccag cagttttacc tcttcccgca
     4141 tctgacggcg ctggaaaacg tcatgtttgg tccgctacgc gtgcgtggcg cgaacaaaga
     4201 agaggcggaa aaactggcgc gtgagctgct ggcgaaagtc ggtctggcag aacgtgcaca
     4261 tcactaccct tccgaacttt ctggtggtca acagcagcgt gtggcgattg cccgcgcgct
     4321 ggcggtgaag ccgaaaatga tgctgtttga tgaaccgact tccgctcttg acccggaact
     4381 gcgccatgaa gtgctgaagg ttatgcagga tctggctgaa gaagggatga cgatggtgat
     4441 cgtgacccac gaaatcggtt ttgccgagaa agtagcttcg cggctgatct ttatcgacaa
     4501 aggccggatt gcggaagatg gcgatccgca ggtattgatc aagaacccgc cgagccagcg
     4561 cttgcaggaa tttttgcagc acgtctctta acttctctgc ctccctttca cccgaaaggg
     4621 aggcacacca gattcctctc atttaaaatc gcccctcccc cagcatctat acttatcttt
     4681 ttgctctgtt ttctcactgg aggagtcatg cggtggatcc tgttcatcct cttctgcctg
     4741 ctgggcgcac ctgcccacgc ggtatccata cccggcgtta caaccacaac gacaacggac
     4801 tcaacgactg aaccggcccc ggaaccggat atcgaacaaa aaaaagcggc ctatggcgca
     4861 ctggcggatg tgctggataa cgacacctcg cgtaaagaat tgatcgacca gttgcgtact
     4921 gtcgccgcga cgccccctgc ggaaccggtc ccgaagatcg tgccgccgac gctggttgaa
     4981 gagcaaaccg tgctgcaaaa ggtcaccgaa gtcagccgcc attatggtga agccctttcc
     5041 gcccgcttcg ggcaacttta tcgcaatatc accggctccc cgcataagcc atttaatcca
     5101 caaaccttca gcaatgcgct gagccatttt tcaatgttag cggtattagt gtttggtttt
     5161 tactggctga ttcgcctgtg cgcactgccg ctgtatcgca aaatgggtca gtgggcgagg
     5221 caaaaaaatc gtgagcgcag caactggttg cagcttccgg cgatgattat cggggcgttt
     5281 attatcgacc tgctgttact ggccctgaca ttatttgtcg gccaggtatt aagcgacaac
     5341 ctgaatgcag gcagtcgcac catcgctttc caacaaagtt tgtttctcaa tgcctttgcc
     5401 ctgattgaat ttttcaaagc cgtactacgc ctgatttttt gcccaaacgt ggcggagctg
     5461 cgtccgttca cgattcagga cgagagcgcc cgttactgga gtcgtcgcct gagctggtta
     5521 agcagtttga taggttatgg cctgattgtg gccgtgccga ttatctctaa tcaggtgaat
     5581 gtacagatag gtgcgctggc gaacgtcatc attatgctgt gcatgaccgt ctgggcgttg
     5641 tacctgatct ttcgtaataa aaaagagatt acccagcatt tgctcaactt tgcggagcat
     5701 tcgctggcct ttttcagcct gtttatccgc gcctttgcgc tggtgtggca ctggctggca
     5761 agcgcctatt ttatcgtgct gtttttcttt tcgttgttcg atccgggcaa cagcctgaaa
     5821 tttatgatgg gtgcaacggt gcgcagcctg gcgattattg gtatcgcagc gtttgtttcc
     5881 ggtatgtttt cccgctggct ggcgaaaacc atcaccctct cgccacatac tcagcgtaac
     5941 tatccggagc tacaaaaacg gctgaatggc tggctttcgg cggcgctgaa aacggcgcgt
     6001 attctgaccg tctgcgtggc agtaatgttg ctgttgagcg catggggatt gttcgatttc
     6061 tggaactggc tgcaaaacgg cgcggggcag aaaaccgtag atatcctgat ccgcatcgca
     6121 ctcattcttt tcttctcggc ggttggctgg acggtgctcg ccagtttgat cgaaaaccgg
     6181 ctggcttcgg atattcatgg ccgcccgcta cccagcgccc gtacgcgtac cctgctgacg
     6241 ctgtttcgta acgcgctggc ggtgattatc agtaccatca ccatcatgat tgtgttgtcg
     6301 gaaatcggcg tcaatatcgc gccattgctg gcaggtgccg gggcattagg tctggctatc
     6361 tcgtttggtt cgcaaacgct ggtgaaagat atcattaccg gggtgtttat tcagtttgaa
     6421 aacggcatga acactggaga tttggtgact atcgggccgt tgaccggcac agtggaacgg
     6481 atgtcgattc gctccgtggg cgtgcgccag gataccgggg cgtatcacat cattccgtgg
     6541 tcttcgataa ccacctttgc taacttcgtc cgcggtattg gttcggtagt ggcaaactac
     6601 gatgttgatc gccatgaaga tgctgataaa gccaatcagg cactgaaaga tgcggtagcg
     6661 gaattgatgg aaaacgaaga aattcgcggg ctgattattg gtgaaccgaa ttttgccggg
     6721 attgtcggct taagcaatac cgcgtttaca ctgcgtgttt cgttcaccac gctgccactc
     6781 aaacagtgga cggtacgctt tgccctcgac agccaggtga aaaaacattt cgacctggcg
     6841 ggcgttcgcg cgccagtgca gacttatcag gtgctgcctg ctccgggcgc gaccccggct
     6901 gaaccgccgc cgccggggga gccaacgctt taacgttggc gattaacaaa acggcggcgc
     6961 tgctcgtcgt tcataaaggt ccaggcaata aagcgactct gcttttgccc ctgggccatc
     7021 tcttttttta ccaccttcac cgcgcccacg tcggtcagag cacgatacaa cggcggtaag
     7081 ttttcaccac gagataccag cgaggtaaac cacatcacct gcttcgcgaa gcctttgctc
     7141 tcttcaatca tctttttgat aaaggcgact tcaccacctt cacaccacaa ctcctgttgc
     7201 tgaccaccaa agttcagtgc atcgtctttg ttcagcccca ggttacggcg tttacgctca
     7261 ctacctgccc gcgctgcagc ggcggaatcg tggaatggcg ggttacacaa ggtcgcgtcg
     7321 tattgctcgt ttttatggat gatgccgtta aaaattgccc cactctcttt ttgccgacgc
     7381 agacgaatgg cgcggttaag ccccggatta gcactgataa tcgcctgcgc actgcttaac
     7441 gcctggctgc tggtttcact accggtaaaa cgccagccat attcatgtac gccaatcagc
     7501 ggataaatac agttcgcacc aacgccgata tccagaatgc tggcattcgc tggaattgtt
     7561 ccgcttgctt cagccagtaa atcggcaagg tgatgaatat aatccgcccg gcccggtact
     7621 ggtgggcaga gaaaaccgtc ggggatatcc cagttcgcta cggcgtaaaa atgggccagc
     7681 aacgccttat tgagcgcctt caccgccagc ggattggcaa agtctacgct ttgctccccg
     7741 gcgggtgtaa gcgtgaggaa ttgcctgagt tcaggattga cctgacaaag cgtggcgaga
     7801 tcgtagcggc tgtgatgacg gttgcgcgga tgcaaccccg gtttctgggc ggacatagca
     7861 ttctccttta agcagcggcg taagataccc gcataacgcg acagggtaaa tatctggctg
     7921 cgtttatcat tccccaaaac cttaacatgg ctatggttta tggcaattca tcataaaaat
     7981 cagcattatg catgtcattg atttgtctta ttttgtaaaa atatatcgtt caaaaaataa
     8041 gcacatctct gtcaattttt tgcgtcattt tcatcgccca ggcgacctac acttatcagg
     8101 cactacccgt tgtatttatg aggatgtcat tatgaaaaag tgcctcacac tactgattgc
     8161 caccgtcctg agcggaatct ctctcacggc ttatgccgcg caaccgatga gtaacctgga
     8221 cagcggtcag ctacggcccg ccggtaccgt ttcggcaact ggcgcatcaa acctaagcga
     8281 tctggaggat aaactggcag aaaaagcgcg cgagcaaggc gcgaaaggtt atgtcattaa
     8341 ttccgcaggc ggaaatgacc agatgttcgg tactgcacca tctacaaata accccaccaa
     8401 cgtaactgca ttcgcctctg gatgcagttt tcgcctgttt tgtaatcaaa aaacatcaaa
     8461 tatgaacgca atgtaaataa atgtatttct ttttcgcgca atgggtgata gaaaatcgcg
     8521 ccaagtgata atgcttatca aaattattat cacttgcacg aacactatca cgagattaac
     8581 agtggcatcg catccgcaga gaggctttct cgtggcagtg aaaatttcaa catataaaaa
     8641 aagtcacctg caaaatggaa aacaatcgca atttccctgc cagacaattt cattcgctca
     8701 cgttctttgc cggtctttgt attggcatca cgcctgtggc tcaggcactc gccgccgaag
     8761 ggcaaactaa cgcggatgac acgctggttg tcgaagcatc aacgccttcg ctttatgcgc
     8821 cacaacaatc tgccgatccg aaattctcgc gtccggtagc ggatactacc cgcacgatga
     8881 cggtgatttc tgaacaagtg attaaagatc agggcgcaac caaccttacc gacgcgctca
     8941 aaaacgtccc cggcgtgggt gcgttttttg cgggtgagaa cggtaactcc accactggcg
     9001 acgccattta tatgcgcggt gctgatacct ctaacagtat ttatattgat ggcattcgcg
     9061 atatcggcag cgtctcgcgc gacaccttca atactgagca ggtcgaagtg attaaagggc
     9121 cttccggcac cgactacggg cgcagcgcgc cgacgggctc gatcaatatg atcagcaagc
     9181 agccgcgcaa tgattccggc attgacgcct ccgccagtat tggcagcgcc tggttccgcc
     9241 gcggcacgct ggacgtcaat caggtcattg gtgataccac tgcggtgcgc ctgaatgtga
     9301 tgggcgaaaa aacgcacgat gccggacgcg acaaagtcaa aaatgagcgt tacggcgtcg
     9361 ccccttctat cgcttttggc cttggtacag cgaatcgttt gtatcttaat tatctgcatg
     9421 tcacccagca caacacgcca gacggcggca ttccgaccat cggtttgccg ggttattctg
     9481 ccccatctgc gggaacagcg gctctgaatc attccggaaa agttgatact cataactttt
     9541 acggcacgga ttccgattac gacgattcga ccaccgacac cgccaccatg cgttttgagc
     9601 acgacatcaa cgataacacc accattcgca atactacccg ttggtcgcgc gtaaagcagg
     9661 attacctgat gacggcgatt atgggcgggg cgtcgaatat tactcagccc accagcgatg
     9721 tgaatagctg gacctggtca cgcacggcga ataccaaaga tgtgagtaat aaaattctca
     9781 ccaaccagac caacctgacc tcgacgttct ataccggttc tatcggtcat gatgtcagta
     9841 ccggcgtgga atttacccgt gaaacgcaga ctaactacgg cgttaatccg gtgacgttac
     9901 ccgcggtaaa tatttatcat cctgacagca gcattcatcc cggcggcctg acgcgcaacg
     9961 gcgcaaacgc caatggtcag acggatacct tcgcaattta cgccttcgat acgctgcaaa
    10021 tcacccgtga ttttgagctg aacggcggga tccgtctgga taattatcat actgaatatg
    10081 acagtgccac cgcctgcggc ggcagcggac gcggtgccat cacctgccca actggtgtgg
    10141 caaaaggttc tccggtcacc accgtcgaca ccgccaagtc gggcaatctg gtgaactgga
    10201 aagccggggc gctgtatcac ctgacggaaa acggtaatgt ctatattaac tatgccgttt
    10261 cccagcagcc tccgggcggc aacaacttcg cccttgcgca gtctggcagc ggtaacagtg
    10321 ccaaccgcac cgattttaaa ccacaaaaag ccaacaccag cgagattggc accaaatggc
    10381 aggttctgga taaacgcctg ttgctcaccg ccgcgctatt ccgtactgat atcgaaaatg
    10441 aagttgagca aaatgatgac ggaacttact cgcaatacgg taagaaacgt gtcgaaggct
    10501 atgagatatc cgtggccggg aatatcacgc ccgcgtggca ggtgattggc ggctataccc
    10561 agcaaaaagc aaccatcaaa aacggcaaag atgttgccca ggatggttcc tcatcgctgc
    10621 cgtatacccc ggagcacgcc ttcaccttat ggagccagta tcaggcaaca gatgatatct
    10681 ctgttggcgc gggcgcacgc tatatcggca gtatgcataa aggttcagac ggcgcggtgg
    10741 gaacgccagc gtttaccgaa ggttactggg tcgccgatgc caaactgggg tatcgggtta
    10801 atcgcaatct cgacttccag ctaaacgttt acaacctgtt tgataccgat tacgtcgcct
    10861 caatcaacaa gagcggctac cgttatcacc cgggcgagcc aagaaccttc ttgctcacag
    10921 ccaatatgca tttctgattc agatgtgggg cgcaggcccc actttttgga gaacttgtat
    10981 gatgtaccac attcccggcg tgttatcgcc acaggacgtc gcccggtttc gcgaacaact
    11041 ggaacaagcc gaatgggtgg atggacgcgt caccaccggc gcacaaggtg cgcaagttaa
    11101 gaacaatcaa caggtcgaca cccgcagcac gttatacgcc gccctgcaaa atgaggtgct
    11161 gaacgcggtt aaccaacatg ctttattctt tgccgcggcc ttgccgcgta ccctttccac
    11221 gccgctgttt aatcgctatc agaacaatga aacctatggt ttccatgtgg atggcgcagt
    11281 acgcagccat ccacaaaacg gctggatgcg tactgacctt tctgccacgc tgtttttaag
    11341 cgatccacaa agctacgacg gcggcgaact ggtcgttaat gacaccttcg gacaacatcg
    11401 ggtaaaactc ccggcaggcg atctcgtgtt gtatccctcc agcagcctgc attgcgtgac
    11461 acccgtaacc cgcggcgtac gagtggcatc atttatgtgg atccagtcga tgatccgtga
    11521 tgataaaaag cgcgccatgc tgtttgaact ggacaacaat attcagtcgc tgaaaagccg
    11581 ctacggcgaa agtgaagaga tcctgtcgct gcttaatctt tatcataatc tgctgcggga
    11641 atggtcggag atctgatgct tgaactgccc aggttcgtcc ctggattagc tacacttaac
    11701 tgtacaagca ttgatatggg gaggacgata tggcatccgg ttgggctaac gatgacgccg
    11761 tcaacgaaca gatcaacagt acaattgaag atgcgattgc ccgcgctcgg ggtgaaattc
    11821 cgcgtggcga aagcctggat gaatgtgaag agtgcggtgc ccccatcccg caagcccgtc
    11881 gggaagccat tcctggcgtg cgcttatgca ttcattgtca gcaggagaaa ggtttacaaa
    11941 aaccagcgta tacaggatat agtcgcagag gttcgaaaga cagccagtta cgttaactac
    12001 aactggcgag atgcatagcg agtataattt gtattttgcg tggcattccg tgcctttaac
    12061 gctgctgtga ctgccacgcg gcataaaaca agtccataag tacaacaaat aaatggttta
    12121 tcagtatgtt agatatcaat caatttattt gaacaaggcg gtcaattctc ttcgatttta
    12181 tctctcgtaa aaaaacgtga tactcatcac atcgacgaaa caacgtcact tatacaaaaa
    12241 tcacctgcga gagattaatt atgaaaacta tcaatactgt tgttgctgct atggctcttt
    12301 caactctgtc atttggcgtg tttgccgcgg aaccggtaac ggcatcccag gcacagaaca
    12361 tgaataaaat cggcgtggtt tctgccgatg gcgcatccac cctcgatgcc ctggaagcga
    12421 aactggctga gaaagccgcg gcagccggtg ccagtggata cagcatcact tccgccacca
    12481 acaacaataa attaagcggt actgcggtaa tttataagta attcgtagga cggataaggc
    12541 gtttacgccg catccggcaa cagatgcctg atgcgacgct accgcgtctt atcagtatct
    12601 acataatctc tgcaatctat tgaatttgca tgtttttgta ggccggataa ggcgtttacg
    12661 ccgcatccgg catttcacaa caagcacttg tcagcaattt gagaacacgg gaaaaaattt
    12721 atctgtttta ccgcccataa gccaccgaga cgttacttct ctatagccaa ttgttcgcgc
    12781 aactccatta aaaggtagcc cagtcgattc ttgcccttac cattaccacc gtctccccag
    12841 taagcatcgt tttccgtatg ctcaaccagt tttgcgggcg cggttgccag caagagcgca
    12901 cgcagttctg gatgctgttc gaatttagcg cgaagtgctt ttcgcatcac ctgttctttg
    12961 accgactccc agtttttacg cagaggctta gaacgatcgc gccccatgcg tgcggcgacc
    13021 ataggggaag aaacccgacg gatctcttcg cggtattttt catcaaggaa tttttgtgcc
    13081 tgaaaatagt gttctgaggt gggccaggtt ttcccgtcaa ccttgatggg ccaggcggca
    13141 aagttagaaa aatcaccgta gtcatcgctg gtgctgtaga aatttatgat ggtgtcttgc
    13201 atgacgtgtt ggatcctttg tgctcgaacg ggcattaaac cgcattatgt tggtggttat
    13261 tgcgagccgc tttccagaaa cagaaaaacc attacccctg aaaaccgaaa aatgccacaa
    13321 tattggctgt ttatacagtg tttcaggttt tctcatggca ttaaccgccg cgcttaaagc
    13381 gcaaattgcc gcctggtata aggcgcttca ggaacagatc cccgacttta ttccccgtgc
    13441 gccgcagcgg cagatgattg cggacgtcgc caaaacgctg gccggagaag aagggcgaca
    13501 tctggcgatt gaagccccca ccggcgttgg gaaaacgctc tcctatttga tccccggcat
    13561 cgccattgcc cgcgaagagc aaaaaacgct ggtggtgagt accgccaacg tggcattgca
    13621 ggatcagatt tacagcaaag atttaccgct gctgaaaaag atcattcccg atcttaaatt
    13681 cactgccgct tttgggcgtg ggcgctacgt ttgcccgcgt aatctgacgg cgctcgccag
    13741 cactgagccc acgcaacagg atctgctggc gtttcttgac gacgaactga cgccaaataa
    13801 tcaggaagag caaaaacgtt gtgcgaagct gaagggcgat ctcgacactt ataaatggga
    13861 tggtctgcgc gatcatactg atatcgctat agatgacgat ctctggcgtc gtttaagtac
    13921 cgacaaagcc agctgcctca accgcaactg ttactactat cgtgaatgcc cgttttttgt
    13981 tgctcgtcgg gagattcagg aagcggaagt ggtggtggca aaccatgcgc tggtgatggc
    14041 ggcgatggaa agcgaagccg tactacctga cccgaaaaat ctgctgctgg tgctggacga
    14101 aggtcatcac ctgccagatg tggcccggga tgcgctggag atgagtgccg aaatcaccgc
    14161 gccctggtat cgactacagc tggacttgtt cacgaaactg gtcgctacct gcatggagca
    14221 gtttcgcccg aagaccatcc caccgctggc gatccctgaa cgtttgaatg cgcattgtga
    14281 agagttgtat gagcttatcg cctcgttaaa caacattctc aatctctaca tgcctgccgg
    14341 gcaggaggca gagcaccgtt ttgcgatggg cgaactgcct gatgaagtgc tggagatctg
    14401 ccagcggctg gcaaaactca ccgagatgct gcgtggtctg gcggagttat ttcttaacga
    14461 tttaagtgag aaaaccggca gccacgatat tgtgcgtctg catcggttga ttttgcagat
    14521 gaaccgcgcg ttggggatgt tcgaggcgca aagcaaactc tggcggctgg cgtctctggc
    14581 gcaatcttcc ggtgcgccgg tgaccaaatg ggcgacgcgg gaagagcgcg aagggcagct
    14641 gcatctctgg tttcactgcg tgggaatacg tgtcagcgat cagctggaaa ggctgctgtg
    14701 gcgcagtatt ccgcacatta ttgtcacctc cgcaaccttg cgttcgctga acagtttttc
    14761 gcgtttgcag gagatgagtg gtctgaaaga gaaagcgggc gaccgttttg tggcgctgga
    14821 ttcccccttt aaccactgcg aacagggcaa aattgttatt ccccggatgc gctttgagcc
    14881 ttccatcgac aacgaagagc agcatattgc cgaaatggcg gcctttttcc gtaagcaggt
    14941 ggagagcaaa aaacatctcg gtatgttggt actgtttgcc agcggacggg cgatgcagcg
    15001 ctttctcgac tatgtgacgg atttacgcct gatgctgctg gtgcagggcg atcagccgcg
    15061 ttatcgctta gttgaactgc accgcaaacg cgttgccaac ggtgagcgta gtgtgctggt
    15121 gggcttacag tcatttgccg aagggcttga tttgaaaggt gatctgctca gccaggtgca
    15181 tatccacaaa atcgcttttc cgcccatcga cagcccggtg gtgatcaccg aaggggaatg
    15241 gctgaaaagc ctcaaccgct atccgtttga ggtgcaaagc ctgccgagcg cctcgtttaa
    15301 cctgattcag caggttgggc gactgattcg aagccacggt tgctggggcg aagtggttat
    15361 ctacgacaaa cgcttgctga ccaaaaatta tggcaagcga ctactggatg cattaccggt
    15421 atttccgata gagcaaccgg aagtccctga aggtatagtt aaaaagaaag aaaaaacgaa
    15481 atccccacgc cgtcggcggc gttaatgatg tgagtcaggt aaggagtcgt aaatggacta
    15541 tcgcaaaatc attaaagaga tcgggcgcgg gaaaaaccac gcgcgcgatt tagaccggga
    15601 tactgcgcgc ggtctgtatg cccatatgct caatggtgaa gtccctgacc ttgagttggg
    15661 cggcgtattg attgcgctgc gtatcaaagg ggaaggggaa gcagagatgc tcggctttta
    15721 cgaagccatg caaaatcaca ccatcaagct gacgccgcct gcaggcaagc cgatgccgat
    15781 tgtcatcccc agttacaacg gcgcgcgtaa acaggccaac ctgacgccgt tgttggcgat
    15841 tctcctgcac aaactcggtt ttcctgtggt ggttcacggg gttagcgaag atccaacccg
    15901 cgtgctgact gaaaccattt ttgaattgat gggtattacg ccaacgttgc acggcggaca
    15961 ggcgcaggcg aagctcgacg aacatcaacc ggtgtttatg ccagtcggcg cgttttgccc
    16021 gccgctggaa aaacaactgg cgatgcgctg gcggatgggc gtgcgtaaca ctgcacatac
    16081 cctggcgaaa ctggcaacgc catttgccga aggtgaggcg ctgcgtcttt ccagcgtttc
    16141 gcatccggaa tacattggac gcgtcgcgaa gttctttagc gatatcggcg ggcgggcgct
    16201 actaatgcat ggtactgaag gtgaagtgta tgctaatccg cagcgctgcc cgcagatcaa
    16261 tctcattgac cgtgaaggga tgcgggtgct gtatgaaaaa caggacactg ctggtagcga
    16321 gttactgcca caagcaaaag atccggaaac cacggcgcag tggattgagc gttgccttgc
    16381 tggcagcgaa ccgattcccg aatcgctgaa aatccagatg gcttgctgcc tggtggctac
    16441 gggtgaagcg gcaactatca gcgacggcct ggcgcgcgtt aatcaggcat tttaattctt
    16501 ttctcagccg gatgagccat gtcatccggc ttttacccca ccgcaatatg aaattcctgc
    16561 atctttattg accttcccac gcccggcgtg cagcataaaa atacaacaaa cacataacat
    16621 aaacaggagt taaccatgga aagtggtcat cgctttgatg ctcagacgct gcacagtttt
    16681 attcaggctg tatttcgtca gatgggtagc gaggaacaag aagcgaaatt agttgccgat
    16741 catttaatcg cggcaaacct ggcagggcat gattcacatg gtattggcat gatcccaagc
    16801 tatgtacgct cctggagtca ggggcacctg caaattaacc atcatgccaa aaccgttaaa
    16861 gagtcggggg cggcggtcac gctcgatggc gatcgcgcat ttggtcaggt cgcggcacat
    16921 gaagcgatgg cgctggggat tgagaaagcg cataagcacg gtattgccgc cgtggcgcta
    16981 cataactcgc atcatatcgg ccgtatcggt tactgggcgg agcagtgtgc agcggcgggg
    17041 tttgtctcta tccactttgt tagcgtggtc ggtattccaa tggtcgcgcc gttccacggt
    17101 cgcgacagcc gctttggcac caatccgttc tgtgtggttt tccctcgtaa agataatttc
    17161 ccgctgttgc ttgattacgc caccagcgcc attgcatttg gtaaaactcg cgtcgcctgg
    17221 cataaaggcg tccccgtgcc gccaggttgc ctgattgacg ttaacggcgt gccgacgacc
    17281 aatccggcgg taatgcagga gtcgccgttg ggggcgctgt tgacctttgc cgaacataaa
    17341 ggctacgccc ttgcagcgat gtgtgaaatt cttggcgggg cgctttctgg cggtaaaacg
    17401 acgcatcagg aaacgttaca aaccagtccc gatgccattc ttaactgcat gaccactatc
    17461 atcatcaacc cggaactctt cggcgcgccg gattgtaacg cgcagaccga agcctttgcc
    17521 gagtgggtga aagcctcgcc gcatgatgat gataagccga ttttgctacc gggcgagtgg
    17581 gaagtgaaca cgcgtcgcga acggcagaag caggggattc cactggatgc gggaagctgg
    17641 caggccattt gtgatgcagc gcggcagatt ggtatgccgg aagagacgtt gcaggctttc
    17701 tgtcagcagt tagccagcta aaaaaaagcc cgtccagtgg cggacgggca aacaagggta
    17761 acataggatc aatgagggtt agagcatatg cgtctgtcgg caaacagaca gggaaatact
    17821 tgtgctggac gtagcgtaaa cgcctgatcc gtccaacggt tcggattttg tagatactga
    17881 taagcgtagc gcatcaggca gttttgcgtt tgttcatcag cctgatgccg ggcatagccc
    17941 ggcataaaag attactgcgc agcggtaggt ttacgcggac ggcggcgacg ttgttgttcg
    18001 cctgccggtt tggcatcgcc gaggcggcga gacggttttt ctgcaggttt cgcgcttgca
    18061 gattttgcgc caccttcccc acggcgtggt tgctgttgac cgcgaccacc accttgccca
    18121 cgaccgccgt cgccacgttg ctggcgaccg ttctggatcg gttcggcttt gattgacggg
    18181 tccggctcat agcccggaat cgcaatgcgc gggatctctt ttttcagcag tttttcgata
    18241 tcacgcagca gtttgtgttc atcaacacac accagcgaca acgcttcacc ggtagcagcc
    18301 gcacgaccgg tacgcccgat acggtggaca taatcttccg gtacgtttgg cagttcatag
    18361 ttgacgacgt gcggcaactc ttcgatatcc agaccgcgcg cagcgatatc ggtcgccacc
    18421 agtacgcgaa tgtcacccga tttaaaatca gccagcgcac gggtacgcgc accttgcgat
    18481 ttattcccgt ggatcgccgc actacggatg ccatctttat tgagctgctc agccagatgg
    18541 ttagcgccgt gtttggtacg ggtaaacacc agcacctgct gccagttccc tttaccaatc
    18601 atgtgcgaca gcaattcgcg tttgcgtttc ttatcaacaa agtgaacgtg ctgggtcacc
    18661 tgatcagacg cggtattgcg gcgtgccact tcgatttcca gcgggttgtg caacagtttt
    18721 tccgccaggg ctttaatatc gtcagagaag gtcgcagaga acagcaggtt ctggcgcttc
    18781 gctggcagtt ttgttaacac gcgacggata tcgtggataa agcccatgtc gagcatgcgg
    18841 tcagcttcat cgaggacgag gatttcaacc tgatccagct tcactgcatt ttgatgttcc
    18901 aggtccagca aacgtcccgg tgttgccacc agaacatcaa cgccgccgcg cagcttcatc
    18961 atttgcgggt taatgctgac accgccaaac accaccagcg aacgaatgtt caaatatttg
    19021 ctgtaatcac ggacgttttc gccaatctgc gcagccagtt cacgggtcgg ggtaagaatg
    19081 agcgcacgta ccggacgacg ccctttggcg tgcggctggc gagtgatcag gtgttgcaac
    19141 agcggcagcg taaagcccgc tgttttaccg gtaccggtct gggcgctagc catcaggtcg
    19201 cggccttcca gcaccgcagg gatcgcctgc tgctgaatag gggtgggttc acggtaaccc
    19261 tgctcggcaa cggcgcgcag gatatcaggg cttaaaccca aagaatcgaa agacataact
    19321 actccgaacc gccccgaccg ttaccggtgt agttttcagg gagatactga aaaggaaaat
    19381 gacaaaaacc acaatgtcat gagggagcgg agtgtagcag tttttgtgac gcagcgcata
    19441 aattatccct gtgactggcg tagctaaaat tcttagccat actggacaac tcccatatgg
    19501 agtcataatc ttaatcaatc atttgattaa gaatggactg tgcgatgaat aatcctgcca
    19561 tgacaatcaa gggtgaacag gcgaaaaaac agctgattgc tgccgcactg gcgcagtttg
    19621 gtgaatatgg aatgaacgcc accacccgcg agatagccgc ccaggccggg cagaatatcg
    19681 ctgccatcac ctactacttc ggttcgaaag aagatttgta cctcgcctgc gcccagtgga
    19741 ttgccgattt tattggcgag cagttccgtc cgcacgccga agaggccgaa cgcttgttcg
    19801 cacaaccaca gcctgatcgc gcagccatcc gtgaactgat ccttcgcgcc tgcaggaaca
    19861 tgattaagct gctcacccag gatgacaccg tcaacctcag caagtttatc tcccgtgagc
    19921 agctctctcc cacggcagcc tatcacctgg tgcacgaaca ggtgattagc ccgctacaca
    19981 gccacctgac acgcctgatt gccgcctgga ccggctgcga cgccaacgat acccgcatga
    20041 tcctccatac tcatgcgttg attggtgaga ttctggcgtt ccgtcttggc aaagaaacaa
    20101 ttctgttacg taccggctgg accgcgttcg atgaagaaaa gaccgaactg atcaaccaga
    20161 cggtgacctg tcatatcgac ctgattttgc aaggattatc gcaaaggagt ttgtagtgat
    20221 gaaaaaacct gtcgtgatcg gactggcggt agtggtactt gccgccgtgg ttgccggagg
    20281 ctactggtgg tatcaaagcc gccaggataa cggcctgacg ctgtatggca acgtggatat
    20341 tcgtacggtg aatcttagtt tccgtgttgg ggggcgcgtt gaatcgctgg cggtggacga
    20401 aggtgatgct atcaaagcgg gccaggtgct gggcgaactg gatcacaagc cgtatgagat
    20461 tgccctgatg caggcgaaag cgggtgtttc ggtggcacag gcgcagtatg acctgatgct
    20521 tgccgggtat cgcgatgaag aaatcgctca ggccgccgca gcggtgaaac aggcgcaagc
    20581 cgcctatgac tatgcgcaga acttctataa ccgccagcaa gggttgtgga aaagccgcac
    20641 catttcggca aacgacctgg aaaatgcccg ctcctcgcgc gaccaggcgc aggcaacgct
    20701 gaaatcagca caggataaat tgcgtcagta ccgttccggt aaccgtgaac aggacatcgc
    20761 ccaggcgaaa gccagcctcg aacaggcgca ggcgcaactg gcgcaggcgg agttgaattt
    20821 gcaggactca acgttgatag ccccgtctga tggcacgctg ttaacgcgcg cggtggagcc
    20881 aggcacggtc ctcaatgaag gtggcacggt gtttaccgtt tcactaacgc gtccggtgtg
    20941 ggtgcgcgct tatgttgatg aacggaatct tgaccaggcc cagccggggc gcaaagtgct
    21001 gctttatacc gatggtcgcc cggacaagcc gtatcacggg cagattggtt tcgtttcgcc
    21061 gactgctgaa tttaccccga aaaccgtcga aacaccggat ctgcgtaccg acctcgtcta
    21121 tcgcctgcgt attgtggtga ccgacgccga tgatgcgtta cgccagggaa tgccggtgac
    21181 ggtgcaattc ggtaacgagg caggacatga atgatgccgt tatcacgctg aacggcctgg
    21241 aaaaacgctt tccgggcatg gacaagcccg ccgtcgcgcc gctcgattgt accattcacg
    21301 ccggttatgt gacggggctg gtggggccgg atggtgcagg taaaaccacg ctaatgcgga
    21361 tgctggcggg attactgaaa cccgacagcg gcaatgccac ggtgattggc tttgatccaa
    21421 tcaaaaacga cggcgcgctg cacgccgtgc tcggctatat gccgcagaaa tttggtctgt
    21481 atgaagatct cacggtgatg gagaacctca atctgtacgc ggatttgcgc agcgtcaccg
    21541 gcgaggcacg taagcaaact tttgctcgcc tgctggagtt tacgtctctt gggccgttta
    21601 ccggacgcct ggcgggcaag ctctccggtg ggatgaaaca aaaactcggt ctggcctgta
    21661 ccctggtggg cgaaccgaaa gtgttgctgc tcgatgaacc cggcgtcggc gttgacccta
    21721 tctcacggcg cgaactgtgg cagatggtgc atgagctggc aggcgaaggg atgttaatcc
    21781 tctggagtac ctcgtatctc gacgaagccg agcagtgccg tgacgtgtta ctgatgaacg
    21841 aaggcgagtt gctgtatcag ggggaaccaa aagccctgac acaaaccatg gccggacgca
    21901 gttttctgat gaccagtcca cacgagggca accgcaaact attgcaacgc gccttgaaac
    21961 tgccacaggt cagcgacggc atgattcagg ggaaatcggt acgtctgatc ctcaaaaaag
    22021 aggccacacc agacgatatt cgccatgccg acgggatgcc ggaaatcaac atcaacgaaa
    22081 ctacgccgcg ttttgaagat gcatttattg atttgctggg tggtgccgga acctcagaat
    22141 cgccgctggg cgcaatatta catacggtag aaggcacacc cggcgagacg gtgatcgaag
    22201 cgaaagaact gaccaagaaa tttggtgatt ttgccgccac cgatcacgtc aactttgccg
    22261 ttaaacgtgg ggagattttt ggtttgctgg ggccaaacgg cgcgggtaaa tcgaccacct
    22321 ttaagatgat gtgcggtttg ctggtgccga cttccggcca ggcgctggtg ctggggatgg
    22381 atctgaaaga gagttctggt aaagcgcgcc agcatctcgg ctatatggcg caaaagtttt
    22441 cgctctacgg taacctgacg gtcgaacaga atttacgctt tttctctggt gtgtatggct
    22501 tacgcggtcg ggcgcagaac gaaaaaatct cccgtatgag cgaggcgttc ggcctgaaaa
    22561 gtatcgcctc ccacgccacc gatgaactgc cattaggttt taaacagcgg ctggcactgg
    22621 cctgctcgct gatgcatgaa ccggacattc tgtttctcga cgaaccgacg tccggcgttg
    22681 atccgattac tcgccgcgaa ttctggctac atatcaacag catggtagag aaaggcgtca
    22741 cggtgatggt caccacccac tttatggatg aagcagaata ttgcgaccgc atcggcctgg
    22801 tgtaccgtgg gaaattaatt gccagcggca cgccggatga tttgaaagcg cagtcggcca
    22861 acgacgagca acccgatccc accatggagc aagcctttat tcagttgatc cacgactggg
    22921 ataaggagca tagcaatgag taacccgatc ctgtcctggc gtcgcgtacg ggcgctgtgc
    22981 gttaaagaga cgcggcagat cgttcgcgat ccgagtagct ggctgattgc ggtagtgatc
    23041 ccgctgctac tgctgtttat ttttggttac ggcattaacc tcgactccag caagctgcgg
    23101 gtcgggattt tactggaaca gcgtagcgaa gcggcgctgg atttcaccca caccatgacc
    23161 ggttcgccct acatcgacgc caccatcagc gataaccgtc aggaactgat cgccaaaatg
    23221 caggcgggga aaattcgcgg tctggtggtt attccggtgg attttgcgga acagatggag
    23281 cgcgccaacg ccaccgcacc gattcaggtg atcaccgacg gcagtgagcc gaataccgct
    23341 aactttgtac aggggtatgt cgaagggatc tggcagatct ggcaaatgca gcgagcggag
    23401 gacaacgggc agacttttga accgcttatt gatgtacaaa cccgctactg gtttaacccg
    23461 gcggcgatta gccagcactt cattatcccc ggtgcggtga ccattatcat gacggtcatc
    23521 ggcgcgattc tcacctcgct ggtggtggcg cgagaatggg aacgcggcac catggaggct
    23581 ctgctctcta cggagattac ccgcacggaa ctgctgctgt gtaagctgat cccttattac
    23641 tttctcggga tgctggcgat gttgctgtgt atgctggtgt cagtgtttat tctcggcgtg
    23701 ccgtatcgcg ggtcgctgct gattctgttt tttatctcca gcctgttttt actcagtacc
    23761 ctggggatgg ggctgctgat ttccacgatt acccgcaacc agttcaatgc cgctcaggtc
    23821 gccctgaacg ccgcttttct gccgtcgatt atgctttccg gctttatttt tcagatcgac
    23881 agtatgcccg cggtgatccg cgcggtgacg tacattattc ccgctcgtta tttcgtcagc
    23941 accctgcaaa gcctgttcct cgccgggaat attccagtgg tgctggtggt aaacgtgctg
    24001 tttttgatcg cttcggcggt gatgtttatc ggcctgacgt ggctgaaaac caaacgtcgg
    24061 ctggattagg gagaagagca tgtttcatcg cttatggacg ttaatccgca aagagttgca
    24121 gtcgctgctg cgcgaaccgc aaacccgcgc gattctgatt ttacctgtgc ttattcaggt
    24181 gattctgttc ccgttcgccg ccacgctgga agtgactaac gccaccatcg ccatctacga
    24241 tgaagataac ggcgagcatt cggtggagct gacccaacgt tttgcccgcg ccagcgcctt
    24301 tactcatgtg ctgctgctga aaagcccaca ggagatccgc ccaaccatcg acacacaaaa
    24361 ggcgttacta ctggtgcgtt tcccggctga cttctcgcgc aaactggata ccttccagac
    24421 cgcgccgttg cagttgatcc tcgacgggcg taactccaac agtgcgcaaa ttgccgccaa
    24481 ctacctgcaa cagatcgtca aaaattatca gcaggagctg ctggaaggaa aaccgaaacc
    24541 taacaacagc gagctggtgg tacgcaactg gtataacccg aatcttgact acaaatggtt
    24601 tgtggtgccg tcgctgatcg ccatgatcac cactatcggc gtaatgatcg tcacttcact
    24661 ttccgtcgcc cgcgaacgtg aacaaggtac gctcgatcag ctactggttt cgccgctcac
    24721 cacctggcag atcttcatcg gcaaagccgt accggcgtta attgtcgcca ccttccaggc
    24781 caccattgtg ctggcgattg gtatctgggc gtatcaaatc cccttcgccg gatcgctggc
    24841 gctgttctac tttacgatgg tgatttacgg tttatcgctg gtgggattcg gtctgttgat
    24901 ttcatcactc tgctcgacac aacagcaggc gtttatcggc gtgtttgttt ttatgatgcc
    24961 cgccattctc ctttccggtt acgtttctcc ggtggaaaac atgccagtat ggctgcaaaa
    25021 cctgacgtgg attaacccta ttcgccactt tacggacatt accaagcaga tttatttgaa
    25081 ggatgcgagt ctggatattg tgtggaatag tttgtggccg ctactggtga taacggccac
    25141 gacagggtca gcggcgtacg cgatgtttag acgtaaggtg atgtaacttc ttatctttcg
    25201 ccagcaaaga cactaccgcc gggccggcaa ggattgccag ccctgcaatt gccagcaaaa
    25261 agttgttttc cagtacacgg gacaacagcc acagcacaat cagcgctgcg ccaaaatacc
    25321 aggtcgtggt cagctcttcc agcacgtctg ccacctcacg ccagcgttgc tcggggtggc
    25381 gctgaaacac caacatcatc actaccgtga cacaatacag tgcgcataat cccacgccga
    25441 cgtgaaccag agtcttactc atccagccca ccaccagcag cgccactacc agtaaatgca
    25501 acataatctg ccagcaactt agaccagttg cgacacgaac acgttgttgc cacttcatgg
    25561 cttctctcct gaagaattta tctctactta ctcagagtac cgaactttac ggaaaattcc
    25621 gtaagaccgc acctttttgt gacgccgact acactatttc aatgagataa gaggcaaaga
    25681 caggaaaatt atgcccgatc aaacacaaca attttcgttc aaggtgctca ccatcaacat
    25741 tcacaaaggt tttaccgcgt ttaaccgacg cttcattttg ccggaacttc gcgacgcggt
    25801 gcgtaccgtc agcgccgata ttgtttgcct gcaggaagtg atgggcgcgc acgaagttca
    25861 tccgctgcat gtggaaaact ggcccgatac ctcgcactac gagtttctcg ccgacacgat
    25921 gtggagcgat tttgcctacg gtcgcaatgc cgtatacccg aaagggcatc acggcaacgc
    25981 cgtactgtcg cgttatccca ttgaacatta tgagaatcgc gatgtttcgg tcgatggtgc
    26041 ggaaaagcgc ggcgtgctct actgccgcat tgtgccaccg atgaccggaa aagcgattca
    26101 tgtgatgtgc gtacatctgg gcctgcgtga ggcgcaccgt caggcgcagc ttgcgatgct
    26161 cgccgaatgg gtgaatgagc taccggacgg cgaaccggta ttggtggcgg gtgatttcaa
    26221 tgactggcgg caaaaagcta atcatccgtt aaaagtgcag gccggactgg atgagatttt
    26281 tacccgcgcc cacggacgcc cggcgcgcac gtttccggtg caatttcctc tactacgact
    26341 ggacaggatc tacgtcaaaa atgccagcgc cagcgcgcca accgcgttgc cgctgcggac
    26401 atggcgacac ctttctgatc atgccccttt aagtgcggag attcatttat gaaatgtagc
    26461 tggcgcgaag gcaataagat ccagttgctg gaaaacggcg agcaatatta tcccgcggtg
    26521 tttaaggcga ttggcgaggc acaagaacgc atcattcttg aaacgtttat ctggtttgag
    26581 gatgacgtcg gcaaacagct gcatgcggca ctactggcag cagcgcaacg cggggttaaa
    26641 gcggaagtct tgctggatgg ctacggttcg ccggatctca gcgatgagtt tgtcaatgaa
    26701 ctgacggcag ctggcgtggt atttcgctac tacgatcccc gccctcgcct ttttggtatg
    26761 cgcaccaatg tgtttcgccg gatgcatcgc aaaattgtgg tgatcgacgc gcgtatcgcc
    26821 tttattggcg ggctgaatta ctccgccgag catatgtcca gctacggtcc agaggctaaa
    26881 caggattacg cggtacgcct tgaagggccg attgtcgaag atattctcca gtttgagctg
    26941 gaaaacctgc ctggacagag cgcggcacga cgctggtggc gacgtcatca caaagcggaa
    27001 gagaaccgcc agccgggaga agcgcaggta ttgctggtct ggcgcgataa cgaagaacat
    27061 cgcgatgata ttgaacgtca ctatctgaaa atgctcactc aggcgcggcg agaagtcatt
    27121 atcgccaacg cctacttctt ccccggctat cgatttttac acgccttgcg taaagcggca
    27181 cggcgcgggg tgcggatcaa actgatcatt cagggcgaac cggatatgcc gattgtcaga
    27241 gtcggtgcgc gcttgctgta taactatctg gttaaaggcg gcgttcaggt atttgagtac
    27301 cgccgccgcc cgctccacgg caaagtggca ttgatggacg atcactgggc gacagtaggg
    27361 tccagtaatc tcgatccgct cagtttgtca ctgaatctcg aagcaaatgt catcatccac
    27421 gatcgtcatt ttaaccagac gctgcgcgat aatctgaacg gcattattgc cgcagattgt
    27481 cagcaggtcg atgagaccat gctgccgaaa cgcacctggt ggaacctgac caaaagcgtg
    27541 ctggcgttcc actttttacg ccacttcccg gcgctggttg gctggcttcc ggcacacaca
    27601 ccacgtctgg cgcaggttga tccgcccgca caaccgacaa tggaaacgca ggatcgggta
    27661 gaaactgaaa acacgggggt aaaaccctga tgagtaaatc acacccgcgc tggcgcttag
    27721 caaagaagat cctcacctgg ctgtttttta tcgcggtgat tgtgttgctg gtggtctacg
    27781 ccaaaaaagt ggactgggaa gaggtctgga aggtcatccg cgactacaat cgcgttgcgc
    27841 tgcttagtgc ggtcgggctg gtggtcgtca gctatctgat ttacggctgc tatgacctgc
    27901 tcgcccgctt ttactgcggt cacaaactgg cgaagcgcca ggtgatgctg gtgtcgttta
    27961 tctgctacgc cttcaacctg acgctcagta cctgggtcgg cggcattggt atgcgctatc
    28021 gtttgtactc ccgactaggg ttaccgggca gcactattac gcggattttc tcgctcagta
    28081 ttaccaccaa ctggctgggc tatattttac tggcagggat tatctttacc gcaggcgtgg
    28141 tggagttgcc ggatcactgg tatgtcgatc aaactacgct gcgtattctc ggcattggct
    28201 tactgatgat tatcgcggtt tatttgtggt tttgcgcttt cgcgaagcac cgccatatga
    28261 ccatcaaagg gcaaaagctg gtgctgcctt catggaaatt cgccctcgct cagatgctga
    28321 tttccagcgt taactggatg gtaatggggg cgattatctg gctgttactt ggtcaaagcg
    28381 tgaactattt ctttgtactg ggcgtgttac tggttagtag tattgctggc gtcatcgtgc
    28441 atattccggt ggggatcggt gtgctggaag cggtgtttat cgcactactg gctggggagc
    28501 atacctcgaa agggagcatt atcgccgccc tgctcgctta tcgcgtcctg tattacttta
    28561 tcccgctgct gctggcgctg atctgttacc tgctactgga aagccaggcg aagaagctac
    28621 gggcgaaaaa tgaagcggcg atgtgaagtt tagttaagtt ctttagtatc tgcatttacg
    28681 actaatgaaa aaaacgcgta tgcctttgcc agacaagcgt tatagctatc gcggcaagta
    28741 tcccaaagaa gccgataaaa tagtaataga gtgttaatgc acctgtaaca gcgcaacgaa
    28801 ccggactcca caactcttca ctatggcatt tccccgccaa ttcaaggagc ttatttcgtc
    28861 catgcgagat aatgcctgac cataacacaa tcattaagca acttattacc caaacgggtc
    28921 gttcactcat taatgcggca ttcatgatga tcaccagggc caaccctgtc aacgtcatca
    28981 taatgataaa acggtgagaa ccaggatcaa cattaaataa taaaccggcc agcatactga
    29041 ttaaaaacat cgcgcccgca gtgctgaaca cgacgataat ggcaggtaca ggagctaaac
    29101 ttgcaacgac aatcgccacc aaagagagta aaaacagata tattacgcct gcgatgacgg
    29161 catttatctt gttgatatcc ttacttaagc catacataca aactaataat aaccctatgg
    29221 cgacatacat tgttggtgaa tcatatttgg ttgccgtaat ttcgagatac ttagctcccc
    29281 aggccagtcc tgcggttgcc accaaaccta aagcggtaat gagcc
//
LOCUS       DAZGLJ010000035        28839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000035 DAZGLJ010000000
VERSION     DAZGLJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.035"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            241..1098
                     /gene="focA"
                     /locus_tag="ACHK49_001428"
     CDS             241..1098
                     /gene="focA"
                     /locus_tag="ACHK49_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO9262100.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1153..3435
                     /gene="pflB"
                     /locus_tag="ACHK49_001429"
     CDS             1153..3435
                     /gene="pflB"
                     /locus_tag="ACHK49_001429"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO9262101.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3627..4367
                     /gene="pflA"
                     /locus_tag="ACHK49_001430"
     CDS             3627..4367
                     /gene="pflA"
                     /locus_tag="ACHK49_001430"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO9262102.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4449..5039)
                     /gene="ycaK"
                     /locus_tag="ACHK49_001431"
     CDS             complement(4449..5039)
                     /gene="ycaK"
                     /locus_tag="ACHK49_001431"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFO9262103.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            5139..6046
                     /gene="ycaN"
                     /locus_tag="ACHK49_001432"
                     /pseudo
     CDS             5139..6046
                     /gene="ycaN"
                     /locus_tag="ACHK49_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(6047..7477)
                     /gene="ycaM"
                     /locus_tag="ACHK49_001433"
     CDS             complement(6047..7477)
                     /gene="ycaM"
                     /locus_tag="ACHK49_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO9262104.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPFLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTASAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(7686..8834)
                     /gene="ycaD"
                     /locus_tag="ACHK49_001434"
     CDS             complement(7686..8834)
                     /gene="ycaD"
                     /locus_tag="ACHK49_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9262105.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            9149..9775
                     /gene="ycaC"
                     /locus_tag="ACHK49_001435"
     CDS             9149..9775
                     /gene="ycaC"
                     /locus_tag="ACHK49_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO9262106.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(9811..10674)
                     /gene="dmsC"
                     /locus_tag="ACHK49_001436"
     CDS             complement(9811..10674)
                     /gene="dmsC"
                     /locus_tag="ACHK49_001436"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO9262107.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(10676..11293)
                     /gene="dmsB"
                     /locus_tag="ACHK49_001437"
     CDS             complement(10676..11293)
                     /gene="dmsB"
                     /locus_tag="ACHK49_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO9262108.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(11304..13748)
                     /gene="dmsA"
                     /locus_tag="ACHK49_001438"
     CDS             complement(11304..13748)
                     /gene="dmsA"
                     /locus_tag="ACHK49_001438"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO9262109.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            complement(13987..15279)
                     /gene="serS"
                     /locus_tag="ACHK49_001439"
     CDS             complement(13987..15279)
                     /gene="serS"
                     /locus_tag="ACHK49_001439"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO9262110.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            complement(15370..16713)
                     /gene="rarA"
                     /locus_tag="ACHK49_001440"
                     /gene_synonym="mgsA"
     CDS             complement(15370..16713)
                     /gene="rarA"
                     /locus_tag="ACHK49_001440"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO9262111.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            complement(16724..17335)
                     /gene="lolA"
                     /locus_tag="ACHK49_001441"
     CDS             complement(16724..17335)
                     /gene="lolA"
                     /locus_tag="ACHK49_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO9262112.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            complement(17490..21596)
                     /gene="ftsK"
                     /locus_tag="ACHK49_001442"
     CDS             complement(17490..21596)
                     /gene="ftsK"
                     /locus_tag="ACHK49_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO9262113.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQSYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            complement(21731..22225)
                     /gene="lrp"
                     /locus_tag="ACHK49_001443"
     CDS             complement(21731..22225)
                     /gene="lrp"
                     /locus_tag="ACHK49_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO9262114.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            22769..23734
                     /gene="trxB"
                     /locus_tag="ACHK49_001444"
     CDS             22769..23734
                     /gene="trxB"
                     /locus_tag="ACHK49_001444"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO9262115.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGSGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            23857..25623
                     /gene="cydD"
                     /locus_tag="ACHK49_001445"
     CDS             23857..25623
                     /gene="cydD"
                     /locus_tag="ACHK49_001445"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO9262116.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            25624..27345
                     /gene="cydC"
                     /locus_tag="ACHK49_001446"
     CDS             25624..27345
                     /gene="cydC"
                     /locus_tag="ACHK49_001446"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO9262117.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFRLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            27387..28091
                     /gene="aat"
                     /locus_tag="ACHK49_001447"
     CDS             27387..28091
                     /gene="aat"
                     /locus_tag="ACHK49_001447"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO9262118.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            28376..28594
                     /gene="infA"
                     /locus_tag="ACHK49_001448"
     CDS             28376..28594
                     /gene="infA"
                     /locus_tag="ACHK49_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO9262119.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
BASE COUNT         7054 a         7510 c         7100 g         7175 t
ORIGIN      
        1 ttattcgcct gatgctccct tttaattaac tgttttagcg gaggatgcgg aaaaaattca
       61 actcatttgt taatttttaa aatttatttt tatttggata atcaaatatt tactccgtat
      121 ttgcataaaa actatgcgag ttacgggcct ataagccagg cgagatatga tctatatcaa
      181 tttctcatct ataatgcttt gttagtatct cgtcgccgac ttaataaaga gagagttagt
      241 gtgaaagctg acaacccttt tgatctttta cttcctgctg caatggccaa agtggccgaa
      301 gaggcgggtg tctataaagc aacgaaacat ccgcttaaga ctttctatct ggcgattacc
      361 gccggtgttt tcatctcaat cgcattcgtc ttctatatca cagcaaccac tggcacaggc
      421 acaatgccct tcggcatggc aaaactggtt ggcggcattt gcttctctct ggggctgatt
      481 ctttgtgttg tctgcggagc cgatctcttt acttccacag tgttgattgt tgttgctaag
      541 gcgagtgggc gcatcacctg gggtcagttg gcgaaaaact ggctaaatgt ctattttggc
      601 aacctggtcg gcgcactgct gtttgtactt ttaatgtggc tttccggcga gtatatgacc
      661 gcaaatggtc aatggggact aaacgtccta caaaccgccg accacaaagt gcaccatact
      721 tttattgagg ccgtctgcct tggtatcctg gcaaacctga tggtatgtct ggcagtatgg
      781 atgagttatt ctggccgtag cctgatggac aaagcgttca ttatggtgct gccggtcgcg
      841 atgtttgttg ccagcggttt tgagcacagt atcgcaaaca tgtttatgat cccgatgggt
      901 attgtaatcc gcgacttcgc atctccggaa ttctggaccg ctgtcggttc tgcaccggaa
      961 aatttttctc acctgaccgt gatgaacttc atcactgata acctgattcc ggttacgatc
     1021 ggtaatatta tcggcggtgg tttgttggtt gggttgacat actgggtcat ttacctgcgt
     1081 gaaaacgatc accattaatg gttgtcgaag tacgcagtaa ataaaaaatc cacttaagaa
     1141 ggtaggtgtt acatgtccga gcttaatgaa aaattagcca cagcctggga aggttttacc
     1201 aaaggtgact ggcagaatga agtaaacgtc cgtgacttca ttcagaaaaa ctacactccg
     1261 tacgagggtg acgagtcctt cctggctggc gctactgaag cgaccaccac cctgtgggac
     1321 aaagtaatgg aaggcgttaa actggaaaac cgcactcacg cgccagttga ctttgacacc
     1381 gctgttgctt ccaccatcac ctctcacgac gctggctaca tcaacaagca gcttgagaaa
     1441 atcgttggtc tgcagactga agctccgctg aaacgtgctc ttatcccgtt cggtggtatc
     1501 aaaatgatcg aaggttcctg caaagcgtac aaccgcgaac tggatccgat gatcaaaaaa
     1561 atcttcactg aataccgtaa aactcacaac cagggcgtgt tcgacgttta cactccggac
     1621 atcctgcgtt gccgtaaatc tggtgttctg accggtctgc cagatgcata tggccgtggc
     1681 cgtatcatcg gtgactaccg tcgcgttgcg ctgtacggta tcgactacct gatgaaagac
     1741 aaactggcac agttcacttc tctgcaggct gatctggaaa acggcgtaaa cctggaacag
     1801 actatccgtc tgcgcgaaga aatcgctgaa cagcaccgcg ctctgggtca gatgaaagaa
     1861 atggctgcga aatacggcta cgacatctct ggtccggcta ccaacgctca ggaagctatc
     1921 cagtggactt acttcggcta cctggctgct gttaagtctc agaacggtgc tgcaatgtcc
     1981 ttcggtcgta cctccacctt cctggatgtg tacatcgaac gtgacctgaa agctggcaag
     2041 atcaccgaac aagaagcgca ggaaatggtt gaccacctgg tcatgaaact gcgtatggtt
     2101 cgcttcctgc gtactccgga atacgatgaa ctgttctctg gcgacccgat ctgggcaacc
     2161 gaatctatcg gtggtatggg cctcgacggt cgtaccctgg ttaccaaaaa cagcttccgt
     2221 ttcctgaaca ccctgtacac catgggtccg tctccggaac cgaacatgac cattctgtgg
     2281 tctgaaaaac tgccgctgaa cttcaagaaa ttcgccgcta aagtgtccat cgacacctct
     2341 tctctgcagt atgagaacga tgacctgatg cgtccggact tcaacaacga tgactacgct
     2401 attgcttgct gcgtaagccc gatgatcgtt ggtaaacaaa tgcagttctt cggtgcgcgt
     2461 gcaaacctgg cgaaaaccat gctgtacgca atcaacggcg gcgttgacga aaaactgaaa
     2521 atgcaggttg gtccgaagtc tgaaccgatc aaaggcgatg tcctgaacta tgatgaagtg
     2581 atggagcgca tggatcactt catggactgg ctggctaaac agtacatcac tgcactgaac
     2641 atcatccact acatgcacga caagtacagc tacgaagcct ctctgatggc gctgcacgac
     2701 cgtgacgtta tccgcaccat ggcgtgtggt atcgctggtc tgtccgttgc tgctgactcc
     2761 ctgtctgcaa tcaaatatgc gaaagttaaa ccgattcgtg acgaagacgg tctggctatc
     2821 gacttcgaaa tcgaaggcga atacccgcag tttggtaaca atgatccgcg tgtagatgac
     2881 ctggctgttg acctggtaga acgtttcatg aagaaaattc agaaactgca cacctaccgt
     2941 gacgctatcc cgactcagtc tgttctgacc atcacttcta acgttgtgta tggtaagaaa
     3001 actggtaaca ccccagacgg tcgtcgtgct ggcgcgccgt tcggaccggg tgctaacccg
     3061 atgcacggtc gtgaccagaa aggtgctgta gcgtctctga cttccgttgc taaactgccg
     3121 tttgcttacg ctaaagatgg tatctcctac accttctcta tcgttccgaa cgcactgggt
     3181 aaagacgacg aagttcgtaa gaccaacctg gctggtctga tggatggtta cttccaccac
     3241 gaagcatcca tcgaaggtgg tcagcacctg aacgttaacg tgatgaaccg tgaaatgctg
     3301 ctcgacgcga tggaaaaccc ggaaaaatat ccgcagctga ccatccgtgt atctggctac
     3361 gcagtacgtt tcaactcgct gactaaagaa cagcagcagg acgttattac tcgtaccttc
     3421 actcaatcta tgtaattaga tttgactgaa atcgtacagt aaaaagcata caataaaggc
     3481 tccacgaaag tggggccttt tttagcacga gagccttttt tgtcagctat ctatacttta
     3541 aggtgactgc caaaacagac tcgacgtagc cttcgagctg cgcaccaaca cggcctcaga
     3601 tgggccacat ctggagaaac accgcaatgt cagttattgg tcgcattcac tcctttgaat
     3661 cctgtggaac cgtagacggc ccaggtattc gctttatcac ctttttccag ggctgcctga
     3721 tgcgctgcct gtattgtcat aaccgcgaca cctgggatac gcatggcggt aaagaagtta
     3781 ccgttgaaga tttaatgaaa gaggtggtga cctatcgcca ctttatgaac gcttccggcg
     3841 gcggcgttac cgcatccggc ggtgaggcaa tcctacaagc tgagtttgtt cgtgactggt
     3901 tccgcgcctg caaaaaagaa ggcattcata cctgtctgga caccaacggt tttgttcgtc
     3961 gttacgatcc ggtgattgat gaactgctgg aagtaaccga cctggtaatg ctcgatctca
     4021 aacagatgaa cgacgagatc caccaaaatc tggttggagt ttccaaccac cgcacgctgg
     4081 agttcgccaa atatctggcg aacaaaaatg tgaaggtgtg gatccgctac gttgttgtcc
     4141 caggctggtc tgacgatgac gattcagcgc atcgcctcgg tgaatttacc cgtgatatgg
     4201 gcaacgttga gaaaatcgaa ctcctcccct accacgagct gggcaaacac aaatgggtgg
     4261 caatgggtga agagtacaaa ctcgacggtg ttaaaccacc taagaaagag accatggaac
     4321 gcgtgaaagg cattcttgag cagtacggtc ataaggtaat gttctaattt ccccgtaaag
     4381 cggcaacttt attgagttgc cgctttttta ttccgcgctt cattctgcta cagcgttttt
     4441 aacacatttt atagtgcatc taccatatcc cgtacctgag ataataacga ctgataatag
     4501 ctcgcatgaa gttcttgccc atcgaatacc actgtattac acaagaaagt gacatcggca
     4561 tcttcaatac caagataact gcacatattt ttcaaataat cgcttatatt tttttcccag
     4621 cccatctgga caaatgattc tttgtctcct ccaaccagcg ccacccaacg aactttattg
     4681 aatggtaatt tgtgcccatc tccataagcc agcccattat tccatactct gtcaatatat
     4741 ccttttagca ttgccgggaa gctataccac cagagaggaa aaaccaccac taacgtgtca
     4801 tgttcaagca gctctgaata aagctgatga acctctggag aataacgttt atccatattc
     4861 ttccagtctg gttcatcttc cggcgtcatc actgggttga aattacgccg atataaatcg
     4921 agttccgtca cctgtatttt ccgctccatt gcccgctgat ggatcgcatc agcaatattt
     4981 gcggtcaatg aatcatgacg aggatgggcc cataccaaat aaatacgttc agactgcata
     5041 attttacctc cacgttaagt tctggagcgc actttacgca ggcggcataa tcctgtaaac
     5101 gtgatgattc aggatgagtt aatgaaggat tttcactaat gcggatgaat atgtctgact
     5161 ttgccacttt ctttgccgtc gcccgtaatc aaagctttcg tgcagcgggc gatgagttag
     5221 gcttatcctc gtccgccatt agccatagta ttaaaacact ggaacaacgt cttaaaattc
     5281 gcttattcaa tcggacaacc cgaagcgtat cgttaacgga agcgggttct aatctctatg
     5341 aacgattgcg cccagctttc gacgaaattc agatcatgtt agatgagatg aatgattttc
     5401 gtctgacacc gacaggaacg ctcaaaatca atgccgcccg ggtagctgca cgtatatttt
     5461 taatgccttt actggttggt ttcacacgtg aatatcctga tattaaagtc gagctcacaa
     5521 ctgacgattc tcttgtcgat atcgtccaac agggctttga cgctggcgtt cgacttagtg
     5581 gcatcgtcga aaaagatatg atcagcgtgg cgatcggccc accagtgaaa ttatgtgtag
     5641 cagcaacgcc agagtacttt gcaagatatg gcaaaccccg ccatcctcat gatctgctta
     5701 atcatcaatg tgttgttttt cgttatccca gcggcaaacc tttccactgg caatttgcaa
     5761 aggagctgga aattgctgtc gcaggaaata tcattctgga tgatgttgat gctgaactgg
     5821 aggccgtttt gatgggtgct ggtatcgggt atctgctcta tgaacagatc aaggagtacc
     5881 ttgatactgg tcgtttagaa tgtgtactgg gagactggag cacagagcgt ccaggcttcc
     5941 agatctatta tcaaaccgcc agtatatgtc ctgcggcttg cgcgcatttc tcgattatgt
     6001 aaaaacgggt cagatctgcc agagtcagcg tcaccgacca caataattat ttactattcg
     6061 ctttacgcgc cagcagcggg aaaatcagtc ctaatcctac cagcacaaac ggcgttgcaa
     6121 cattcagcgc caactggaag gtccactctg cagtgaaggc ttccattttc gggaagatcc
     6181 ccgtcaaaca ggcaaaggcg gtaaaggcaa aacaccaaat cccgacggtc attgccagag
     6241 gcttattacg aataaagaca tattctggtt tatatttctg cgccaggcga acgactgcaa
     6301 taaatgcaac aaatacccat aaataacgca gcggcatcac taccgagtta agattcaaca
     6361 gccatttata gagattgttc atatcaccga tgccgagagt cggcagcata atcagaattg
     6421 ccaccagcac cagggtcaga aaatagccat taacgggcgt accagaagcg ttggtacgac
     6481 ataaactggc tggaatatat ttgctgtcag catcacctaa tagcacttta agcggggcat
     6541 caatcgagaa taccagcgca gctacttgtc ccagggtatt cgcaatggcg taaatcacca
     6601 ttaaagtatt acccatgttg taatactcgc ccagcttctg aaaggcgtaa tattgaccgt
     6661 tagtcattaa gtcatccggg atattacgcg agtcaaacat catccccatc gccagtgagc
     6721 ccagaatggc acaaaccgca accatcaccg ccaggcatag catccctttt ggaaattctt
     6781 ttcctgggtt gcgcgtttga ttaacgtaag gagaaatctt ctctgctccg ccaaccgcga
     6841 aaaccagcat tgaaatagtg gtaatgtagg taaagtcgat atgaggaatg aacgtttccc
     6901 aggtaatgtt tgtggtcgca atatgcactt cagtaatcgc agacgcggtt accgccatcg
     6961 ccacatacag gagcgacata acaaacatcg caatccctgc cacagaaccg acgattttca
     7021 gcgatttcat accgcgtgaa gcaacccaca taaagaagat aaacagcacc agcgttaacc
     7081 cctgtaacgc tacgactgaa tattctttga ttagcgaacc gtcgcctttc atcgcccaac
     7141 cgagcgcaat cagaattgcc tggggttttt gtgccagaaa gggaatatgc accacccagt
     7201 aggtccacgc ggcgagataa gccagtccgg gtcccatcgt gtgtttaatc caggtactga
     7261 ccccgccctt cccatctttg aaggtcgagc ctaactgacc aacaattagc gcataaggtg
     7321 tgaaatagag tgcaaagata aacacccatg aaaaaacaac caccagcccc tggttggcat
     7381 agttattaac aacgttgcca aaaccccaga cagtgataaa agacatcagc gcaatgttgt
     7441 accatcgcaa ctgtttttcc tgaacattcc cagccataga caaatccttc gccaaaatta
     7501 aaggggcatc tgtaaaacga gggattatgc gcggcaaata agaagtgcgg aaatagcgaa
     7561 atgcaaaatt ataacctgct gcacatttta tttttaaacg aataaaattt ggaataaagc
     7621 aagtcagata gaagaaaaaa aaagcagagt gtgaaacagt aagttcacac tctgcttgaa
     7681 ttcatttaca cgtgagcaac gggtttcggc gtatgaccgg cgttgcgcag cagcatcagc
     7741 aaatagataa acgatacgct ggcgatcatg ataaacaata aattatcgga gaaattctgc
     7801 attagcatag cggtaaatga cgggccaagc agacttccca cagtatagct caacagtaag
     7861 gcctggttca tcgccaccag ttgatgatgt tcaactttct cgcaagccca tgccatcgcc
     7921 accggatata gcgtaaagcc agcggcaccg aggatgaata acgctggggc catcgccgcc
     7981 tggctaagca tcgcgatact gccgagaatg acgacaaaga cctgaacacg caacaccagc
     8041 agtcgaccaa acttatccgc cagacgtcca atcggccatt gtccaaggat acccgcactg
     8101 accagtaccg ccatccagaa accaatgctg gcattgctca cccctttgtg attgaggtac
     8161 agcggcatca ggccatatag agatcccaga acgattcctg agataatgca gccattcacg
     8221 ccaagccgcg cctgacggag ttttagcatt gacgtaattg acgtcgaatc atggttttca
     8281 gcctgctgat tcagcacacg cgtaaacaac agcggtaaga tccctgccaa cgtcaaacct
     8341 gtaacccacg gcaatacgga catcagctcg gttgaaactt tgctgaccag taactggcct
     8401 aaaaacgttc ccacgtaata aaccatcata tacgcagcaa gcaaacgccc acggttacgt
     8461 gacgtcccac tgcacatcag cgcgctctca acaaccaccc aaatcatggc acagccgacg
     8521 cccgcgacaa aacgccaagc caaccagctc cagaatccaa tcatcaggcc aaggccggca
     8581 cagccagcgg caaaaatgaa ggaggccaga taatagctgc ggttaaagcc aatgcgctta
     8641 atgacatacc ctgtcagcaa tgtaccgaca aggttgccgg taaaatagga tgagctgaca
     8701 acgcctacct gccatgtgga catgtgttcc tgggcgagcc aaagcggcac gagtgtattt
     8761 aacaccgcga tcgccagagt caacaaaagc aggccagaca gcaaaagcat gacaggccgg
     8821 gtatacgtgg acatgaatta aaaccgtgag gaagttcaaa tttcatgcgc atcatgccac
     8881 cgccaaaaac attgtcaatg ggtgaaaata cgcgcatatc gcagcggcgg tattatcgat
     8941 ttatattact cattcataac gctaatgctg aagagatgat taatctcatc tgattgtttt
     9001 tattgatcta acacaaattt catacgcttc gttcagtcga aaaatttcat ggtcacagca
     9061 aactcatatt tcccgtctat gctttcagag caatgcatca cgtctgaccg atttcagacg
     9121 taccactgac tctcgaaagg agaagaggat gaccaaaccg tatgttcgtc ttgataaaaa
     9181 tgatgctgcc gttttgcttg ttgatcatca ggctggttta ctttcccttg tacgggatat
     9241 cgaacccgat aagtttaaaa ataacgtgct ggcgctgggt gacctggcta agtatttcaa
     9301 cttacctact attcttacca ccagttttga aaccggtcct aacggtccat tagttccaga
     9361 actgaaagca caattccccg atgcacctta cattgctcgc ccgggaaata ttaacgcctg
     9421 ggataacgaa gattttgtaa aagcagtcaa agcgacaggt aaaaaacagt taattattgc
     9481 cggtgtggta accgaagttt gcgtggcatt cccggcgctt tcagccattg aagaagggtt
     9541 tgatgtcttt gttgtgactg acgcgtcagg gacctttaat gaaattaccc ggcattcggc
     9601 atgggatcgc atgtcgcaag caggtgcgca attgatgaca tggttcggcg tggcgtgcga
     9661 gctgcaccgt gactggcgta atgatattga aggactggcg acgttgttct ctaaccatat
     9721 tcctgattat cgtaatttga tgaccagtta tgacacgtta acgaagcaga aataaaagag
     9781 aaccggggcc gaaaccccgg ttgttactga ttagcttgcg acggccatcc ccacggtcat
     9841 atgcaaacca tagaatacgc cgcgaccgat taattcccct gccagaagca gaatgaatga
     9901 gacggatagt agcggaaccg caggctgata accttttagc tgtggtgcaa tccacaagca
     9961 cagggcaacg gccaaaagca cgatccgcca ggacatcagc gcaccatagt ccggcaccag
    10021 cgctgcggcc tgctgcacag aactatgaat agttgccagc tctgtgccct gcatcactga
    10081 catcacgcca ctcaccacca gtgccagtac agaaatggct ggcagcagac gcatcgccca
    10141 gccatcgacg ccagccagac tcaacaacag gtaacccagt aacgggccgc ccataaacat
    10201 cgtcaggaag aagcccatcg gcgtccagat gctgtaccag gtcggaacgg tatcaatgct
    10261 gttatacaca cgcaccatca tccagacaaa gatgacgcca agaaccatcg tcactatcag
    10321 ccacagcgta cgcaatgccg gtgacagctt tttcagcatt gccagcaacc agccgatgcc
    10381 gcctacggca aaaaagatcg aaccgctggc gatttcgtta ctgagtgctg aagcccctac
    10441 ccggttgagc gagttaaaag cgcgcattgg tgaaccaaga tggagcatag aggcgataaa
    10501 gccaatgccc atcagcaccc ataaaccaaa catgcaggcg ataacacgct gctgggcttc
    10561 tgctcgcagg tcgcctttga gcagcgccaa agccagaacg ataaaaccac ctgctacaca
    10621 ttgcccgaag accgtgaaga tcatcagcgg ccattcatgc catccacttc ccatctcaca
    10681 cctccttcgg gtttgccaga tagccagtgg tatccccggt cgggcggcta ttggcattgg
    10741 gtttgatcac aatattcggt ttggtaaagt gagctcgcgg caacggcgca acggccgcca
    10801 gatcgccatg ttttttacgc agctcgtcga tagggccgaa atccagcgcc cgcagcggac
    10861 aagattcaac acagatcggc tttttaccct ccgcaacacg gtcataacaa ccatcgcatt
    10921 tggtcatatg gcctttcgtt tcgttatatt gcggtgcgcc atacgggcaa gccatatggc
    10981 agtagcggca gccaatgcac acatcttcat cgaccacaac aaaaccatct tcacgtttat
    11041 gcatcgcacc gctcgggcag actttagtac aagccggatc ttcgcagtgg ttacatgaaa
    11101 tcgacagata gtaggcaaac acgttctggt gccagacacc gttatcttcc tgccagtcgc
    11161 cgccagcata ttcataaatg cggcggaagc tgacttctgg cgtcaaatct ttgtagtctt
    11221 tacaggccag ctcgcaggtt ttgcaaccgg tgcaacggct ggaatcaata aaaaatccat
    11281 actgggttgt catcggttac tccttacacc ttttcaacct gaacaaggtt tgtatgtgac
    11341 ggattcccct tagcgagagg agacggacgt tgagtggtca gtacgttgat acaaccgccc
    11401 ttatcgacac gttttgtatc cgggtcatac caggcacctt cacccagtgc gaccacaccc
    11461 ggcatcattc gtggcgtcac tttcgcctca atatgaacct caccacgatc gttaaagatc
    11521 ctgactttat cgccgttgtt gataccgcgt ttttgcgcat caagcgggtt gatccacatt
    11581 tcctgacggc aagctgcttt cagcacatca acgttgccgt aagttgagtg aacgcgagat
    11641 ttatagtgga aacccgtaag ctgcagcgga tactgtttgt tcagcggatc ctgataactt
    11701 tcaaagcccg gcgtgtagat cggcagtgga tcgatcacat cgccttctgg caattcccag
    11761 gtagcggcaa tgtcagccag cgcctgcgaa taaatctcaa ttttgcccga tggcgtagtc
    11821 agtgggtttg cctgcggatc ttcacggaag gctttataag caacgtgatg cccttgtggg
    11881 tcgcgctttt taaagatccc ctgcttgcgg aactcttcaa acgttggcag ttcaggaatc
    11941 gcttcccgcg actgggcata cagatgccgc atccactctt cctgggtacg gccttcagta
    12001 aactgttgct caacgccaag acgttttgcc agttcgctgg tcatttcata gatggtctta
    12061 cattcaaagc gcggtttaat cacctgatcg ttgaaaatca cgtaagacat attcccgcag
    12121 gatgcatcca gtgcaaagtc catctgttcg gaagcggtgc agtcaggcag caggatgtca
    12181 gcatatttcg ccgatgaggt catgtggcag tcgataacca caatcagctc gcacttctta
    12241 tcatcctgaa ggatttcatg ggtacggttg atttcagaat gctggttaat caggcagtta
    12301 ccggcatagt tccagatcat tttgatcggc acatccagct tatctttccc gcgtacacca
    12361 tcacgcagcg ccgtcatttc cgggccacgt tcaatggcat cggtccacat aaacatcgaa
    12421 atgctggtct ggatcgggtt ttccaaggtc ggcatacgga caaacggtaa gctgtatgaa
    12481 ccttcacgcg cgccgctgtt gcctccgtta ataccaacgt taccggtcag aatcgccagc
    12541 atcgagatag cacgggttgc gatttcaccg ttagcgtgac gctgcgggcc ccatccctgg
    12601 ctgataaacg ccggtttggt actgccgatt tcacgagcca gtttgatgat tttgtctgcc
    12661 ggaacaccgg tgatttgtga ggcccattcc ggtgttttag ccacgccatc tggcccttca
    12721 cccagaatat aagctttata gtggccattt ttcggcgcac tggctggcag ggttttctca
    12781 tcgtagccaa cgcaatattt atcgaggaat gcctgatcca ccaggttttc agtgatcatg
    12841 acgtacgcca gaccgttaac cagtgcggca tctgttcccg gacgaatagg gatccactca
    12901 tcttcgcgcc cggcaccggt gtcggtatag cgcggatcaa tgatgatcat gcgggcatta
    12961 gatttctggc gtgcctgttc aagatagtaa gtcaccccac cgccactcat tcgcgtttcg
    13021 ccagggttat taccaaacag cactaccagc ttactgtttt cgatatccga cgggctgttg
    13081 ccatctgccc agccgccgta ggtatagttc aaaccttccg caatttgcgc ggaggagtag
    13141 tcgccgtaat ggttcagata gccgccgcag cagttcatca gccgcgcgac cagggtattt
    13201 cccggcggcc aggagcgggt catggtgccg cccagcgtac cggtgccata gttcagatag
    13261 atagactcgt tgccgtactc tttgatcagg cgctgcatat tggtcgcgat gatgtcgtag
    13321 gcttcttccc agctaatgcg ctcgaatttg ccttcaccgc gcgccccgac tcgtttcatc
    13381 ggatatttca ggcggtccgg attgtagaca cggcgacgca tggaacgccc acgcaggcag
    13441 gcgcgaacct ggtgcaggcc gtcgtaattg tcatcgccgg tattgtccgt ttcgacatat
    13501 ttgatttcac cgtccacgac gtgcatacgt agcgggcagc gactaccaca gttaactgta
    13561 caggcgctcc agataacctt ttcgtctgat tttgttggaa tggcgctatc gacagcgtgc
    13621 gcaatccgac taaaaggtaa tgttaatgcg ctgctggcca ttgccaggcc gccgatcgct
    13681 gtcgttttta ccaaaccacg gcgactcacc tcagcagcca ataccgcatc agggattttc
    13741 gttttcataa tggctcactc aagcttgctc acgattgatt ccagtaacaa accgtattgt
    13801 gtaattattt ctatagcagc tcgctatata ggcttgtata catcgaataa tttgaaaatc
    13861 aaagagatag gtagattttc actcgaaagt agtattacgt gtaagtgagg agtaattatt
    13921 gttcggtatc aaagggagac aaaaaaagcg cccgcaggcg ctttttagat tcagaaaaat
    13981 tgggtattag ccaatatatt ccagtccgtt catatacgga cgcagaactt ctggtacttc
    14041 aatacgacca tcagcctgct gatagttttc cattactgca accagcgtac gaccaacagc
    14101 cagtccagaa ccgttcaggg tatgaaccag acgggttttc ttgtccgact tgctgcggca
    14161 gcgtgcctgc atacgacgcg cctggaaatc ccaaacgttg gagcaggaag agatctcacg
    14221 gtaggtgttc tgtgccggga tccatacttc caggtcgtaa gttttgcaag cgccaaagcc
    14281 catgtcgcca gtgcaaagga tgattttacg gtacggcagg cccaacaact gcaggacttt
    14341 ttctgcatga ccagtcatct cttccagcgc cgccattgag tcttccgggc gcacgatctg
    14401 caccatttca actttgtcga actggtgcat acggatcaga ccacgggtgt cacgaccata
    14461 tgaaccggct tcagaacgga agcatggggt gtgggcggtc atcttaattg gcagatcatc
    14521 ttcatcgatg atttcaccgc gcaccaggtt agtcagcgga acttctgccg ttgggatcag
    14581 cgcatagtta ctggtgtctg cttcttcttc cagcggacga gtatgaaaca gatcgccagc
    14641 aaatttcggc agctgacccg taccgtacag cgtgtcctgg ttaaccaggt acggaacata
    14701 gttctcactg tagccatgct gttcggtatg cagatccagc ataaactgcg acagtgcgcg
    14761 gtgcatgcga gcaatctgcc ctttcattac cacaaagcgg gaaccagtca gcttaactgc
    14821 agctgcaaag tcgaggccag agtgcatttc acccagcgtc acatggtcac gaacttcaaa
    14881 gtcaaactca cgcggggtgc cccagcggct gacttcgacg ttgtcatttt cgtctttacc
    14941 taccggcact tcatctgcag gcaggttagg gatggtcagc gcgatatcgc gaatttcagc
    15001 ctgtaaagca tccagctcgg cttttgctgc atccagctct tcgcccagtt tgttcacttc
    15061 cagacgtaaa ggctcgatat cttccccgcg cgctttcgcc tggccaatgg atttcgatcg
    15121 ggagttacgc tccgcttgca ggttttccgt tttgacctgc aatactttac gacgctcttc
    15181 aagagcgccc agcttatcta catccagctt aaagccccgg cgtgccagtt tttcagcgac
    15241 tgcgtctggc tcattacgca gcagattggg atcgagcatg cttatcctgt gcttatcgaa
    15301 ttaaatggga gatgtggcca cagcctgcga ccacagggat acagtaacaa cattaccgca
    15361 acgataacat taacggtagc gttttatggg gctattttga tcctgttcag ccagccaggc
    15421 gagcttttcg ccaatcttgc cttcaaggcc cctgtttgtc gggaaataat agcgtgtttg
    15481 tgctatttcc ggcgggaagt aaacctcacc ggcagcataa gcgtttgctt catcatgagc
    15541 gtaacgatat tcctgcccgt agcccatttc cttcattaat ttcgtcggcg cattacgcaa
    15601 atgaaccggc acgtcataat ccggacgttc gcgagcatcc gccagcgcag ctttaaacgc
    15661 agtgtagaca gcgttgcttt ttggcgcgca ggccaggtaa acaatcgcct gagcaatggc
    15721 gcgttcacct tccgccgggc caacgcgagt aaagcaatcc caggccgcaa ttgccacctg
    15781 catcgcccgt gggtcggcat tgccgacatc ttcggaagca attgccaggc aacgacgcgc
    15841 gacatataac ggatcgccac cagcggtaat aattcgcgca taccagtaga gcgccgcatc
    15901 gggtgcgcta ccacgtaccg acttatgcag tgcggaaatg agatcgtaaa agcgatcgcc
    15961 tttgttatca aagcgggcgc ttcgctcacc ggcgatttcg gtcaataatt caggcttcag
    16021 gacccgctta ccgctatcat tgacttcggc catatccgcc atcatttcca gcgtatttaa
    16081 cgcccggcgc gcgtcgccat tcaccagttc agcaatggcg cgtcgtgttt catctggcag
    16141 aacaatatcc tgaccaccat aaccacgggt cttgtcttcc atcgcctgag ttagtacttg
    16201 ctcaatatcc tctgtaccca gggatttcaa cagatagaca cgggcacggg aaagcagtgc
    16261 cgaattaagc tcaaacgacg ggttttcagt ggttgcgcca ataaaagtga tggtgccgtc
    16321 ttcaatatgt ggcagaaatg catcctgctg gcttttgttg aatcggtgca cttcgtcaac
    16381 aaaaagaata gtgcggcgac ctgcattgcg gttttgccgg gcgcgctcga tcgcctcgcg
    16441 aatctctttc acgccagagg tgacggcaga aatacgttcc acatcagcgt tcgcatagcg
    16501 ggcaatcact tcagcgaggg ttgttttgcc ggtacccggt ggcccccaga ggatcataga
    16561 atgtaaatgc ccggcttcga tagcgcgcgg caacggcttc cccgcagcca gcaaatgttg
    16621 ctggccgata tactgtgcta aattttctgg ccgcatacgc gcggccagag gttgaaaagt
    16681 attatccgaa aaatcgagcg acagattgct cactcaggtg cctctactta cgttgatcat
    16741 ctaccgtgac gccttgcggc ggggtgaagg taaatttcgc tgcatccaca gccccatttt
    16801 gctgggattt cagttgataa ctgctgcgct gatcgtcctg ctccaccgcg ctaaactgat
    16861 ggattgtgcc atcacgtccc acgttaatgg tgaactgctt cagattgcca ttgctggctt
    16921 tcggcgtcag gacaaagtca tcgccattct gtttgatatt gtactgctgc cagtcgctgg
    16981 actggttgcg ggcaatcagc ataaacggcg tattaccggt ggcatctttc agccaggttg
    17041 ccgtagcttg ctcaacgaac gggttataga accacagtgt tttaccgtca gaaaccagaa
    17101 tgctttcatc aggttgtgtc atatgccagt tgaataagtt tggacgtttc acccacagat
    17161 cgccctgacc ttcctgcacc gccgcgccgc taccgtcagt cactttttgt gtgaagctgg
    17221 cgtggaagct gctgacttta tccaggcggc ttttcagatc gcttgcggca tcagcccaaa
    17281 cgctgcttgc tactaagctt gagagtaatg cacaggtgat ggcaattttt ttcatcatta
    17341 ttcctcaaat tacgtcactc ccgatcggga gttccctcta ctctctattc caggctaata
    17401 atcagcttga ggaaagaaga aaatactgaa ttttcagttg atagagtgcc ggatgcgacg
    17461 ctaccgcgcc ttatccggca atgcattaat tagtcaaacg gcggtggggc cagcacttca
    17521 cgattaccgt tgtgcccctg ttcgctgaca atcccctgcg cttccatctg ttcgataata
    17581 cgcgctgcac ggttataacc aatgcggaac tgacgctgta cgccagaaat tgacgctttg
    17641 cgtttttcag tgacaaactg caccgcctga tcgaacaacg gatccagttc ttcagcgcca
    17701 tcgaaaccgc ccgcgccacc ttcgctttca ctgtcggaag tgatgccatc aacatactgt
    17761 gggcgaccac gcgctttcca gtcctgcacc acggcatgaa cttcctgatc gcgaacaaaa
    17821 gcaccatgga cacgtaccgg caacgtggag ttcggcccag agtagagcat atcccccatc
    17881 cccagcagtg attccgcgcc agcctgatca agaatggtac gtgagtcaat cttactggat
    17941 acggtaaagg cgatacgggt cggaatattc gctttaatca gaccagtaat aacatcaacc
    18001 gatggacgct gagttgccag tacgaggtgg atacccgcgg cacgggcttt ttgcgccaga
    18061 cgtgctatca gctcttccac ttttttacct accgtcatca tcaggtcggc aaattcgtca
    18121 accaacacca caatgtatgg ttcttttttc agcaccggat gctgggcatc catactgtca
    18181 cccggcttcc agtacgggtc tggaatcgga cgcatcatgc gatcggcttc agcaattttt
    18241 tcgttataac ccgccagatt acgcacaccc agcgcagaca tcagtttata ccgacgctcc
    18301 atctcgttaa cacaccagcg cagcgcgttg gcggcatctt tcatatcagt aacgacttcc
    18361 gttaacagat gcggaatgcc ttcataaacc gaaagctcca gcattttcgg gtcgatcatg
    18421 atgaaacgca catcttctgg ctgtgcttta taaagcatgc tcaggatcat cgcgttcaca
    18481 ccgacagatt taccggaacc ggtagtcccc gcaaccaaca agtgcggcat tttcgccaga
    18541 tcggcaacca ccggctcacc ggcgatatct ttacccagca ccacggttaa tggcgacgga
    18601 ttatcgcgga atttggcgtt atccaaaact tcgcgcagat aaacggtttg tcgtttttta
    18661 tttggtaact ccagacctac atagggtttg ccaggaataa cttcaacgac acgcaccgcc
    18721 accgtcgaaa gtgaacgggc aaggtcccgt gacaagttag aaatgcgcgc cgcttttacg
    18781 ccaggagcca ggttaagttc aaagcgagtg ataaccggcc ccggagagta attgacgaca
    18841 tcggctttaa tacggaaatc agccagacgc gcttccacca ggcgagccat ttgttcaagc
    18901 gcaaaggtat ctaccggctc cacttcgctc ggcggcggtg tcagcaaatc cagagaaggc
    18961 agcggcgtcg tcggtttatg caacggacgg ctgtcgccat tacgcatcaa cagcggatga
    19021 agcagggtat cctgctgctg cggtgcaacc ggttgttgcg gctgttgata ctgcggctgc
    19081 ggagcaactg gttgttgcgg ctgctgatat tgcggctgtg gcgcaaccgg ttgttgcggc
    19141 tgctgatatt gtggctgcgg cgcaacctgc tgttgtggct gctgatactg cggctgcggc
    19201 gcaaccggct gttgcggctg ctgatattgc tgctgtggcg gaactggttg ttgcggctgc
    19261 tgatactgct gctgcggtgc aaccggttgt tgcggctgct gtacaggttc aacaattggc
    19321 gtaaacaacg gttcgtgtgg accatcatcc agcaacgctt tcattggcga aaattcaaaa
    19381 tcatccagcg agaacggatt cgccccagcc ggttgttcgc cggaataacg ttgttgttga
    19441 gtttgcgcaa actgacgagc cagttcagcc tctgccgcag catctgcatc ttctgcgttt
    19501 acgggcacat catgttgata ctgttcgcca tagcgttgct gctgtgtctg ggcgaactga
    19561 cgtgccagtt catcctgctg catcgcatcg atttcatcat cgttatactg atcgccagaa
    19621 tcatactgat tgcgctgggc ttcacgggct ttctcttccg ccgcacgctg tgagggcagc
    19681 ttaataccgt aagacgccag ttcacgacga gttggcacgc ggatacgttt cggtcgtggc
    19741 aactgcgggc caatcccctc tttgacttgc ggacgcggtc caccgctatt tgccagactg
    19801 aagactggcg cggcaacggt tgcggcagcc cccgtcgcca gtgtcgcttt tttcacgcca
    19861 gatgccagcg gagaaacagc ggcagcggct tctactggag gtactgctgc aacagaaggc
    19921 gctttcaacg aagatttgat cggttctggt tctttaaccg gttctggaat cggttgatac
    19981 caggccgcaa gttgttcacg ttcacgggct cgcttctctt ccacttcttc aaaatagtaa
    20041 agcggcggac gcgcgggttt tgtctcttct acaacgggtt caggctccac aacaggctgc
    20101 tgttcaacgg attgcggctg ttggtacaac ggctcctgag cggctggttg ctgataagtt
    20161 tgctcagtct ggtatgtaga ctgtggagca aaagtggatt gctgctcttc ggcttgccag
    20221 gcgttacctg ccaccggttg ttctggcgca ggggcgtaat acggctgttg tgcaggctgt
    20281 tccggcgcag ttgcgtaata cggttgctga acaggttgtt ccgccgcagg agcgtagtaa
    20341 ggttgttgcg caggttgttc agctgcaggt gcataatacg actgctgcgg ctgtactggt
    20401 tgttgcagcg gctcattata ttgcactgca ggctgcgcat attgtgactg ctgtgggtaa
    20461 ccttccggtg caggagcaat aaccggctct cccgtttgtg gacccggtac aggctgccag
    20521 gctactgtag gttgcgaggg tggaacatca acagaggcaa caggcggcgt ctgagtcaca
    20581 ggttcaaccg gcgcagccca gctttgtgtc gccgtggtag cagcagctgc tacagcgaca
    20641 ggttcggtaa ttggcgcacc gtttaataat ggatcgtatt cgtcatattc tggctgcgtt
    20701 gcacgattgc ccgaaaatag gacgtcgtcc gggtcggcag ccacaccgcg tgcagtgtag
    20761 gtaatctctt cgtcatcatc catccgctta ccggagaaca acgcagcgtc tgtttgccgc
    20821 cccatcggat taatgaattt ttccgccaac cgtttacgac gcgctagcgc gccgcgaaga
    20881 atacgggcac ggcgtgattc atgctgtttg ccgtggtttt catcttcata ctcttcgtca
    20941 tcttcatatt catcttcatc gacccaggta tcatcgcgac gggtacgatt actggcgaag
    21001 gtgagaatgt ttaaaatcca gccgccgagt ttttcagcaa tggtcaccca tgaccaaccg
    21061 gtgaacagcg tcaggcccgc agcccaaacg cagagcagcg caatagttcc cccgctactg
    21121 tgtagcagtg gttgtagcgt agtacttagt aagctgccaa tgacgccacc ggaggcaaaa
    21181 taccagatat cgtcagcgtt gattgccgcc agaccacagg aggtaaggat gagcgccaaa
    21241 acgccaatga tgcgtagcga aacggcaaaa taatcaatgt attcgtcgct ggactgatga
    21301 cgccaggcaa accaacaacc gccgacaata atgacgggaa tggtgtaagc catcacgcca
    21361 aaaataaaga acagcgtatc tgccaaccac gcaccgggca tcccacctaa attatggata
    21421 ggttcatgcc aggccgtttg cgaccagctg gggtccgaag ggttaaagct tagtaaggca
    21481 gccatcaacc agacggcaaa caggacaata aggatcagca acgcctccag aaggcggcgg
    21541 ccgctactta actttgtcaa tgtgacttct ttgtcttcag tgtattcctg gctcaagaaa
    21601 ggctctccag gttacaggct tttcctgccc gatgctaaaa cggacaacag taccaggtta
    21661 ccccagtact gttgctgtat ggattaacag gagtgtaatc aaaatacgcc gattttgcac
    21721 ctgttccgtg ttagcgcgtc ttaataacca gacgattact ctgcttgact tcttccataa
    21781 caacgtatgt ccgtgtgtca ttgacgccag gcagacgcag cagggtttcc cccagcaact
    21841 tacggtaagc tgacatatcc ggcacgcgtg ttttcaacag gtagtcgaaa tcaccagata
    21901 ctaaatgaca ctcctgaatt tcttcaagtt tttgtacagc ggtattgaat tgttcaaaca
    21961 catccggtgc gccacgattc agagtaatct caacgaatac cagaagtgat gcatccagat
    22021 aatgggggtt cagcagcgcc gtatagccct gaataaaccc ttgtctttcc agccgacgca
    22081 cacgctcaag gcacggcgtt ggggaaagtc ccacacgttt agaaagctcg acgttagaaa
    22141 tacgcccatc cttttgcaac tcattaagaa tgttacgatc gatacggtcg agatctttgc
    22201 cagggcgctt cttgctatct accattatta ttgtctctct gtattccttc cctactcctg
    22261 tctgaccctg aaacatcact gttcagagtc gctacccgtc acgatagacc gaaatcatga
    22321 agattcacgg cgcgcaatgc ctgggtctac ggtgagaaga ccgttgcctt gtggcagcta
    22381 tcgacatcac gaatggcgtc tgtccacgcc atacgtagat ttcactagca cggtaaacat
    22441 ggtatttaca tgcatggtta tgcgcatata agaatactgt ttttttcttc ctcgaatgtt
    22501 ttcgcaaaac accaggggat tgtcaaagca aaacatcgat ttttagtaca acatgccaga
    22561 tattcattaa caggggcgtt tattcatcat ttaatcggct tataaagcgg gaaatttaag
    22621 tagcagaggt gataatgacg ggagaatttg acctgcttat cgatgctgcc gattgcacaa
    22681 attgttaaca aaatcgtcat actctttttt tacgtctgta aattccctac aatcctgccc
    22741 attgtctgcc aacaactatg gggatctcat gggcacgacc aaacacagta aactgcttat
    22801 cctgggttca ggcccggcgg gatacaccgc tgctgtctac gcggcgcgcg ccaacctgca
    22861 acctgtgctg attaccggca tggaaaaagg cggccaactg accaccacca cggaagtgga
    22921 aaactggcct ggcgatccaa acgatctgac cggtccgtta ttaatggagc gcatgcacga
    22981 acatgccacc aagtttgaaa ctgagatcat ttttgatcat atcaacaagg tggatctgca
    23041 aaaccgtccg ttccgtctga atggcgataa cggcgaatac acttgcgacg cgctgattat
    23101 tgccaccgga gcttctgcac gctatctcgg cctgccctct gaagaagcct ttaaaggcag
    23161 tggggtttct gcttgtgcaa cctgcgacgg tttcttctat cgcaaccaga aagttgcggt
    23221 catcggcggc ggcaataccg cggttgaaga ggcgctgtat ctgtctaaca tcgcttcgga
    23281 agtgcatctt attcaccgcc gtgacggttt ccgcgcggaa aaaatcctta ttaagcgtct
    23341 gatggataaa gtggagaacg gcaacatcat tctgcacacc aaccgtacgc tggaagaagt
    23401 gaccggcgat caaatgggtg tcactggcgt tcgtctgcgc gatacgcaaa acagcgataa
    23461 catcgagtca ctcgacgttg ctggtctgtt tgttgctatc ggtcacagcc cgaatactgc
    23521 cattttcgaa gggcagctgg aactggaaaa cggctacatc aaagtgcagt cgggtattca
    23581 tggtaatgcc acccagacca gcattcctgg cgtctttgcc gcgggtgacg tgatggatca
    23641 tatttatcgt caggccatta cttcggccgg tacaggctgc atggcagcac ttgatgcgga
    23701 acgctacctc gatggtttag ctgacgcaaa ataattttac aaatcagtaa caaaagtaaa
    23761 gaaggcgaca ccatgcgact atgggtcgcc tttatttttt ccccgttgta acattgctct
    23821 gcaaataatt ctgataactc acctgctaag cgtgcaatga ataaatcccg tcaaaaagag
    23881 ttaacccgct ggttaaaaca gcaaagcgtc atctcccaac gttggctgaa tatttctcgt
    23941 ctgctgggct ttgtaagcgg catattgatc attgcccagg cctggttcat ggcgcgaatt
    24001 ctgcaacata tgattatgga gaatattccc cgtgaagccc tgctgcttcc ctttacgtta
    24061 ctggttctga cctttgtact gcgcgcatgg gtggtctggt tacgcgaacg ggtgggttat
    24121 cacgccgggc agcatatccg ctttgccatc cgccgtcagg ttctcgaccg tctgcaacaa
    24181 gcagggccag cgtggattca gggtaaacct gcggggagct gggcgacgtt ggtactcgag
    24241 caaattgacg atatgcatga ttactatgca cgctacctgc cgcaaatggc gctggcagtg
    24301 tcggtgccgt tgctgattgt ggtggcgatc ttcccctcta actgggctgc ggcgctcatt
    24361 ctgctgggca cagcaccgtt aattccgttg tttatggcgc tggttgggat gggggctgcc
    24421 gatgctaacc ggcgtaactt tctcgctctt gctcgcttaa gtgggcattt cctcgatcgc
    24481 ctgcgcggca tggaaacatt gcgtattttt ggtcgtggtg aagctgaaat tgaaagtatt
    24541 cgttctgctt cggaagattt ccgccaacgg acaatggaag tgctacggct ggcgttttta
    24601 tcctccggca ttctcgaatt ttttacctcg ctgtcaattg ctctggtggc ggtctacttt
    24661 ggtttttcct atctcggcga gctggatttt ggtcactacg ataccggtgt gacgctggct
    24721 gcgggttttc tggccctgat ccttgcgcca gagtttttcc agccattgcg cgatctcggt
    24781 acgttttatc atgctaaagc ccaggctgtt ggtgcagctg acagtctgaa aacgtttatg
    24841 gaaaccccgc tcgcccatcc gcagcgtggc gaggtggaat tagcatcgac cgatccggtg
    24901 accattgagg ccgaggatct gtttatcacg tcgccggaag gtaaaacgct ggccggaccg
    24961 ctgaacttta ctttgccagc aggccaacgt gcagtgttgg ttggtcgcag cggttcaggt
    25021 aaaagctcac tgctgaacgc gctttctggt tttctctcat atcagggatc attacgaatc
    25081 aacgggatag aattacgcga tttatcacca gaatcatggc gtaaacatct ctcctgggtt
    25141 gggcaaaacc cacaattacc ggcagcaaca ttgcgggata acgtactact ggcgcgacct
    25201 gatgccagcg aacaagaatt acaagcagcg ctggataacg cctgggtcag cgagtttcta
    25261 ccgctcctgc cacaaggcgt tgatacacct gttggcgacc aggctgcccg cctttccgtg
    25321 gggcaggcgc agcgcgtggc ggtggcccgt gcgttactaa atccctgttc gctattactg
    25381 ttggatgaac ccgctgccag ccttgatgct cacagtgaac agcgcgtaat ggaggcgctg
    25441 aatgccgcct ctctgcgcca gacaacgtta atggtcaccc accagttaga agatcttgct
    25501 gactgggatg tcatttgggt tatgcaggat ggccggatta ttgagcaagg acgttacgcg
    25561 gaattaagtg tggctggtgg cccattcgcc acattactgg cccatcgtca ggaggagatt
    25621 taaatgcgcg ctttgctacc ctatctggca ctgtataaac gtcataaatg gatgttaagt
    25681 cttggtatcg tgctggcaat tgtcacgctg ctcgccagta tcggtctgtt gacactttcc
    25741 ggctggttcc tctcggcctc agcggttgcg ggggttgccg gactgtacag cttcaactat
    25801 atgctacccg ctgcgggcgt gcgtggcgca gcaatcaccc gtactgccgg gcgttatttt
    25861 gaacgtctgg taagtcacga cgcgactttc cgcgtgttgc agcatctgcg catttacacc
    25921 ttcagcaaat tgctgcccct ctcccctgcc ggactggcgc gctatcgtca gggcgaattg
    25981 ctcaatcgcg tggtggcgga tgttgatacg ctcgatcatc tttatctgcg cgttatctcg
    26041 ccgctggtgg gcgcttttgt ggtgattatg gtggtgacaa tcgggttaag tttccttgat
    26101 ttcaccctcg cctttaggct gggcggcatt atgttactga cgcttttcct gatgccaccg
    26161 ctgttttatc gtgcgggaaa aagcaccggg caaaatctga ctcatcttcg cggacagtat
    26221 cgccaacaac tgacggcctg gctgcaaggg caagctgagc tgaccatttt tggcgccagc
    26281 gatcgttatc gcacgcaact agagaataca gaaattcaat ggctggaagc gcaacgccgt
    26341 caatctgaac tgaccgcatt gtcgcaagcg ataatgctgc tcattggcgc gttagcggtg
    26401 atcctgatgc tgtggatggc gtctggcggc gttggcggca atgctcaacc cggcgcgtta
    26461 attgccctgt ttgtcttctg cgcgttagcc gcgtttgaag cactggcacc agtaacgggt
    26521 gcatttcagc atctggggca agtcattgcc tctgccgttc ggatcactga tttaacggat
    26581 caaaaaccgg aagtcacctt ccctgatacc caaactcgtg ttgccgatcg cgtttcgctg
    26641 acattacggg atgttcagtt cacttatccg gagcaatccc aacaggcact taaagggatt
    26701 tctcttcagg taaacgccgg ggaacatatt gcgattctcg ggcgaaccgg atgcggcaaa
    26761 tcaacactgt tacaactgct gacccgcgct tgggacccgc aacagggaga gattttgctt
    26821 aacgatagcc ccatagccag cctgaatgaa gcggctctac gacagaccat cagcgttgtt
    26881 cctcagcgag tgcatctgtt tagcgccacg ctgcgtgata atctgttact cgcctcgcct
    26941 ggcagtagtg atgaggctct ggcggagagc ttgcgtcgcg ttggtctgga gaagctgctc
    27001 gaggatgcag gtctcaacag ttggttaggt gaaggcggac gccagctctc cggtggtgaa
    27061 ctgcgccgtc tggctatcgc ccgtgcgctg ttacatgatg cgccactggt gttgctggat
    27121 gaacctaccg aaggcttaga tgccacaacc gaaagccaga tccttgaatt gcttgcagaa
    27181 atgatgcgtg agaaaacggt gttaatggtc acccatcgac ttcgcggact ctctcgtttc
    27241 caacaaataa tagtgatgga caacgggcaa attattgagc aaggtactca cgcagagctg
    27301 cttgccagac aggggcgtta ttaccagttc aagcagggtt tgtaagctat tattgaacga
    27361 tccgacttgc gtggagtttt gcggtcatgc gcctggttca gctttctcgc cattcaatag
    27421 ccttcccttc cccggaaggc gcattacgtg agcctaacgg cctgctggca cttgggggcg
    27481 atcttagccc tgcgcgcctg ttaatggctt accagcgtgg tatttttccg tggttttctc
    27541 caggcgaccc catcctctgg tggtcgcccg atccccgcgc ggtgctatgg ccagaatcac
    27601 tgcatatcag ccgtagtatg aagcgatttc ataaacgctc gccctatcgt gtcacgatga
    27661 attacgcttt tggtcaggtc attgaaggct gtgccagcga tcgcgaagaa ggaacctgga
    27721 tcacgcgtgg cgtggtcgaa gcctaccatc gccttcacga actcggccat gcccactcca
    27781 ttgaagtctg gcgtgaagat gagcttgtcg gcggtatgta cggcgtggcc cagggaacgc
    27841 tattttgtgg cgagtccatg ttcagccgga tggaaaatgc gtctaaaacg gcgcttctgg
    27901 tattctgtga ggaatttatc ggtcatggcg gtaagcttat cgactgccag gtccttaacg
    27961 atcacacagc atcgcttggt gcctgcgaaa ttccccgccg ggattacctt aattatctca
    28021 atcaaatgcg cctcggacga ttgccgaata atttctgggt accacgatgc ttgttttcac
    28081 cacaagaatg aatgttttcg gcacatttct ccccagagtg ttataattgc ggtcgcagag
    28141 ttggttacgc tcattacccc gctgccgata aggatttttt cgcgtcaggt aacgcccatc
    28201 gtttatctca ccgctccctt atacgttgcg cttttggtgc ggcttagccg tgtgttttcg
    28261 gagtaatgtg ccgaacctgt ttgttgcgat ttagcgcgca aatctttact tatttacaga
    28321 acttcggcat tatcttgccg gttcaaatta cggtagtgat accccagagg attagatggc
    28381 caaagaagac aatattgaaa tgcaaggtac cgttcttgaa acgttgccta ataccatgtt
    28441 ccgcgtagag ttagaaaacg gtcacgtggt tactgcacac atctccggta aaatgcgcaa
    28501 aaactacatc cgcatcctga cgggcgacaa agtgactgtt gaactgaccc cgtacgacct
    28561 gagcaaaggc cgcattgtct tccgtagtcg ctgattgttt taccgcctga tgggcgaaga
    28621 gaaagaacga gtaaaaggtc ggtttaaccg gcctttttat tttgtgatat gtatgaagta
    28681 ctttggaagt ataagtccgt aacttgtctc gatgtagata tactgcacaa cttgtttaca
    28741 gtatgagcaa acaataatta tcattaaaat tcttcttgac cgttttactc caactcccta
    28801 tagtagcgcc ccgttgccac ccatgaggtt tggtagcaa
//
LOCUS       DAZGLJ010000036        28402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000036 DAZGLJ010000000
VERSION     DAZGLJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.036"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            455..1594
                     /gene="wza"
                     /locus_tag="ACHK49_001449"
     CDS             455..1594
                     /gene="wza"
                     /locus_tag="ACHK49_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO9262120.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1600..2043
                     /gene="wzb"
                     /locus_tag="ACHK49_001450"
     CDS             1600..2043
                     /gene="wzb"
                     /locus_tag="ACHK49_001450"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO9262121.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            2046..4208
                     /gene="wzc"
                     /locus_tag="ACHK49_001451"
     CDS             2046..4208
                     /gene="wzc"
                     /locus_tag="ACHK49_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO9262122.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4301..5140
                     /gene="wcaA"
                     /locus_tag="ACHK49_001452"
     CDS             4301..5140
                     /gene="wcaA"
                     /locus_tag="ACHK49_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO9262123.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5143..5631
                     /gene="wcaB"
                     /locus_tag="ACHK49_001453"
     CDS             5143..5631
                     /gene="wcaB"
                     /locus_tag="ACHK49_001453"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO9262124.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5628..6845
                     /gene="wcaC"
                     /locus_tag="ACHK49_001454"
     CDS             5628..6845
                     /gene="wcaC"
                     /locus_tag="ACHK49_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO9262125.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVVKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITCTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6820..8037
                     /gene="wcaD"
                     /locus_tag="ACHK49_001455"
     CDS             6820..8037
                     /gene="wcaD"
                     /locus_tag="ACHK49_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO9262126.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8048..8794
                     /gene="wcaE"
                     /locus_tag="ACHK49_001456"
     CDS             8048..8794
                     /gene="wcaE"
                     /locus_tag="ACHK49_001456"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO9262127.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            8810..9358
                     /gene="wcaF"
                     /locus_tag="ACHK49_001457"
     CDS             8810..9358
                     /gene="wcaF"
                     /locus_tag="ACHK49_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO9262128.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9384..10505
                     /gene="gmd"
                     /locus_tag="ACHK49_001458"
     CDS             9384..10505
                     /gene="gmd"
                     /locus_tag="ACHK49_001458"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO9262129.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10508..11473
                     /gene="fcl"
                     /locus_tag="ACHK49_001459"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10508..11473
                     /gene="fcl"
                     /locus_tag="ACHK49_001459"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO9262130.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVMRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11476..11955
                     /gene="gmm"
                     /locus_tag="ACHK49_001460"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11476..11955
                     /gene="gmm"
                     /locus_tag="ACHK49_001460"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO9262131.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            11952..13175
                     /gene="wcaI"
                     /locus_tag="ACHK49_001461"
     CDS             11952..13175
                     /gene="wcaI"
                     /locus_tag="ACHK49_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO9262132.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPERIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLHNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGELCETFSGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13178..14614
                     /gene="cpsB"
                     /locus_tag="ACHK49_001462"
     CDS             13178..14614
                     /gene="cpsB"
                     /locus_tag="ACHK49_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO9262133.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPTGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14807..16177
                     /gene="cpsG"
                     /locus_tag="ACHK49_001463"
                     /gene_synonym="manB"
     CDS             14807..16177
                     /gene="cpsG"
                     /locus_tag="ACHK49_001463"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO9262134.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16313..17707
                     /gene="wcaJ"
                     /locus_tag="ACHK49_001464"
     CDS             16313..17707
                     /gene="wcaJ"
                     /locus_tag="ACHK49_001464"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO9262135.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17709..19187
                     /gene="wzxC"
                     /locus_tag="ACHK49_001465"
     CDS             17709..19187
                     /gene="wzxC"
                     /locus_tag="ACHK49_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO9262136.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVACVAVFLLSDAI
                     GDMLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPALHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWSSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19333..20613
                     /gene="wcaK"
                     /locus_tag="ACHK49_001466"
     CDS             19333..20613
                     /gene="wcaK"
                     /locus_tag="ACHK49_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO9262137.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDSGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKSLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLELMKRSNITTSKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTIGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQVMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVKSVLEHIREAK"
     gene            20610..21830
                     /gene="wcaL"
                     /locus_tag="ACHK49_001467"
     CDS             20610..21830
                     /gene="wcaL"
                     /locus_tag="ACHK49_001467"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO9262138.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQILRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21841..23235
                     /gene="wcaM"
                     /locus_tag="ACHK49_001468"
     CDS             21841..23235
                     /gene="wcaM"
                     /locus_tag="ACHK49_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO9262139.1"
                     /translation="MPFKKLSRRTFLTASSVLAFLHTPFARALPARQSVNINDYNPRD
                     WIASFKQAFSEGQTVVVPAGLVCLNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ACNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNVEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23410..24303
                     /gene="galF"
                     /locus_tag="ACHK49_001469"
     CDS             23410..24303
                     /gene="galF"
                     /locus_tag="ACHK49_001469"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO9262140.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGELSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSK"
     gene            24675..25760
                     /gene="rfbB"
                     /locus_tag="ACHK49_001470"
     CDS             24675..25760
                     /gene="rfbB"
                     /locus_tag="ACHK49_001470"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO9262141.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEQSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVENVKSGAYQSWIAQNYEGRQ"
     gene            25760..26659
                     /gene="rfbD"
                     /locus_tag="ACHK49_001471"
     CDS             25760..26659
                     /gene="rfbD"
                     /locus_tag="ACHK49_001471"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFO9262142.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVRSIRPDIIVNAAAHTAVDKAESEPEFAQLLNATSVEAIAKAANEVGAWLIHY
                     STDYVFPGNGDMPWRETDATAPLNVYGETKLAGEKALQEYCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQKFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            26717..27595
                     /gene="rfbA"
                     /locus_tag="ACHK49_001472"
     CDS             26717..27595
                     /gene="rfbA"
                     /locus_tag="ACHK49_001472"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO9262143.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGLRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNYVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            27600..28142
                     /gene="rfbC"
                     /locus_tag="ACHK49_001473"
     CDS             27600..28142
                     /gene="rfbC"
                     /locus_tag="ACHK49_001473"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFO9262144.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSEIAEFVYKTTNYYHPESEGCIKWDDSFLMIDWPNK
                     PIAISEKDKKGSSILRLNDY"
     gene            28132..>28402
                     /locus_tag="ACHK49_001474"
     CDS             28132..>28402
                     /locus_tag="ACHK49_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001430002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9262145.1"
                     /translation="MTIDVEKKLQKIKEYHSVLELAIIQGANAIFPVLVFPFFLITLG
                     ENIFSSIAVGEVLALYVLIFSLYSFDIISVQKVISSVTKDEIFKVY"
BASE COUNT         7140 a         6845 c         7584 g         6833 t
ORIGIN      
        1 cctaaagaaa ctcctaaaaa ccatattgaa tgacacttaa tataattctt aaaaatagcc
       61 aattaccgaa ttgttacctt gcctgctatt ccgttagctg taacacttcc tcctgcatta
      121 ttggagagcc aatattcaat taccataatg tcaccttata ttcggaagtt aatttccgca
      181 ataacccttt ctcatcaacg attgtatgca gagagggttt gaaaaattaa gctaaatcag
      241 tgtattggta gctaaaaagc caggggcggt agcgtgtctg gatgcctgaa agaccagtct
      301 gaattattct gtcaatagcc tgcggatgaa atcaattttt ttaggactga tgccagttaa
      361 atttttattc aattaactgc aaggtaaatg tgcatggcga cattgctatt aatagtgcac
      421 aggataatta ctctgccaaa gtgataaata aacaatgatg aaatccaaaa tgaaattgat
      481 gccattattg gtgtcagtaa ccttgataag cggttgcaca gtacttccgg gcagcaatat
      541 gtcgacgatg ggcaaagacg tcatcaaaca gcaggacgct gatttcgatc tcgacaaaat
      601 ggtgaatgtt tatccgctga ccccgcgcct gattgaccaa ttacgcccac gcccgaatgt
      661 agcgcgcccc aatatgacgc tggaaagcga gatcgcgaat tatcagtatc gcgtcgggcc
      721 gggggacgtt cttaatgtca ccgtctggga tcacccggaa ctcaccacgc cagccggtca
      781 gtaccgcagc tccagcgaca ccggcaactg ggtacagcct gacggcacta tgttttaccc
      841 gtatatcggc aaggttcacg tagtcgggaa aacgctcgct gaaatccgca gtgatattac
      901 cgggcgctta gcgacgtaca tcgctgaccc gcaggtggac gttaatatcg ccgccttccg
      961 ctcgcaaaag gcctatatct caggtcaggt gaataaatcc ggtcaacagg cgatcaccaa
     1021 cgtaccactg accattctcg acgccatcaa cgccgcaggt ggcctgaccg acaccgctga
     1081 ctggcgcaac gtggtgctaa cacacaatgg tcgtgaagaa cgcatttctt tgcaggcgct
     1141 gatgcaaaac ggcgacctca atcagaaccg actgctttat cccggcgata ttctctacgt
     1201 gccgcgtaat gatgatctga aagtatttgt gatgggtgaa gtgaagaaac agagcaccct
     1261 gaaaatggac tttagcggca tgaccctgac tgaagctctg ggcaatgccg aaggcatcga
     1321 catgaccacc tccaacgcca gcggcatctt tgtcattcgt ccgttgaaag gcgagggcgg
     1381 gcgtaacggc aagattgcca atatctacca gctggatatg tccgatgcga cgtcgctggt
     1441 gatggcgaca gaattccgcc tgcaacctta tgacgtggtg tatgtcacca ccgccccggt
     1501 ttcccgctgg aaccgtctga tcaatcagtt gctgccaact attagcggtg tccgttacat
     1561 gacggataca gccagcgaca ttcataactg gtaatcgtca tgtttaacaa catcttagtt
     1621 gtctgtgtcg gcaatatttg ccgttccccg acggcggaac gcttactgca acgttatcac
     1681 ccggagctga aagtggagtc cgctggactc ggcgcgctgg tcggtaaggg cgctgatcct
     1741 accgctatca gcgtcgccgc agaacatcaa ctgtctctgg aaggtcactg tgcacggcaa
     1801 atcagccgcc gcctgtgtcg caactatgac ctgattttga ccatggaaaa gcgccatatc
     1861 gaacgcttat gcgagatggc acctgagatg cgcggcaaag tgatgctgtt tggtcactgg
     1921 gataacgaat gtgaaatccc cgatccgtat cgcaaaagcc gggaaacgtt tgcagcggtg
     1981 tacacattac ttgaacggtc tgcccgccag tgggcgcagg cattgaacgc agagcaggta
     2041 taagaatgac agaaaaagta aaacaacatg ccgctccggt aacgggcagt gatgaaatcg
     2101 atattggtcg cctggtcggc accgtcattg aagcgcgctg gtgggtgatt ggcatcaccg
     2161 ctgtatttgc cctctgtgct gtggtttaca ctttcttcgc cacgccgatt tatagtgccg
     2221 acgcactggt acaaatcgaa caaaacagcg gcaattcgtt agtgcaggac attggctcgg
     2281 cattagccaa caaaccgcct gcatcggacg ccgagatcca gttgattcgt tcgcgtctgg
     2341 tgcttggtaa aacggtggac gatctagacc tcgatattgc ggtgagcaaa aacacgttcc
     2401 cgattttcgg tgcgggctgg gatcgtttga tgggacgtca gaacgagacg gtgaaagtga
     2461 ccacctttaa ccgcccgaaa gagatggcgg accaggtgtt tacgcttaat gtgctggaca
     2521 ataaaaacta caccctgagc agtgatggcg gctttagcgc ccgtgggcaa gcgggccaga
     2581 tgctgaaaaa agaaggcgtc acgctgatgg ttgaagccat tcacgcccgc ccgggcagtg
     2641 agtttaccgt caccaaatac tccacgctgg ggatgatcaa tcaactgcaa aacagcctga
     2701 cggtaacgga gaacggcaaa gacgcaggcg tactgagcct gacttatacc ggtgaagatc
     2761 gtgaacagat ccgcgacatt cttaacagca tcgcccgtaa ctatcaggaa caaaatattg
     2821 agcgcaaatc ggcggaagcg tcgaaaagcc tcgctttcct cgcgcaacag ttaccggaag
     2881 tacgtagccg cctcgatgtt gccgaaaaca aactgaatgc cttccgtcag gataaagatt
     2941 ctgttgatct gccgctggaa gcgaaagcgg tgctcgattc gatggtgaac atcgacgcgc
     3001 agttgaacga actgaccttt aaagaggcgg aaatctccaa gctgtacacc aaagttcacc
     3061 ccgcgtaccg cacgctgctg gagaaacgtc aggcgctgga agacgaaaaa gccaaactta
     3121 atggtcgcgt aacggcgatg ccgaaaaccc agcaggaaat tgtccgtctg acccgcgatg
     3181 tcgaatctgg tcagcaggtc tatatgcaac tgctgaataa agagcaggag ctgaaaatca
     3241 ccgaagccag caccgtcggc gatgtgcgca ttgttgaccc ggcaatcact cagcctggtg
     3301 tgctaaaacc gaagaaaggg ctgattatcc tcggcgcgat tatccttggc ctgatgctct
     3361 ctatcgtggg tgtgctgctg cgctcgttgt ttaatcgcgg catcgaaagc ccgcaggtgc
     3421 tggaagaaca cggtatcagc gtctatgcca gcatcccgtt gtcggaatgg cagaaagcgc
     3481 gcgatagcgt caaaaccatc aaagggatta aacgctataa acagagccag ctactggcgg
     3541 tggggaatcc aaccgatctg gcgattgaag ccatccgcag tcttcgtacc agtttgcact
     3601 tcgcgatgat gcaggcgcag aacaatgtgt tgatgatgac cggggttagc ccgtcaatcg
     3661 gtaaaacctt tgtctgcgcc aacctggcgg cggtgatcag ccagaccaat aaacgcgtgt
     3721 tgttgatcga ctgcgatatg cgcaaaggct acacccacga gctgttgggc actaataacg
     3781 ttaatggcct gtcggaaatt ctgattggtc agggcgatat tactaccgct gctaaaccga
     3841 cctctattgc caaatttgac ctgatcccgc gcggtcaggt gccgccaaat ccttctgaac
     3901 tgttgatgag cgaacgcttt gccgaactgg tgaactgggc gagtaaaaac tatgacctgg
     3961 tgttgattga tacgccgccg attctggcag tgaccgatgc ggcaattgtt ggtcgtcatg
     4021 ttggaaccac gttaatggtg gcgcgttatg cggtcaacac attgaaagaa gtggaaacca
     4081 gtctgagccg ctttgagcaa aatggtattc cggtgaaagg ggtgattctg aactccatct
     4141 tccgccgcgc cagcgcgtat caggattatg gctattacga atacgaatat aagtcggatg
     4201 cgaaataacg acgcctgcat tcgcaccgcc ccgtaggccg gataaggcgc tcacgccgca
     4261 tccggcaagc aaaccagctc ataagccggg agaacaacct atgaaagaca atccgctgat
     4321 ctcaatctat atgccaacct ggaaccgcca acaactggcg attcgggcaa taaaatcggt
     4381 tttgcgccag gactacagca actgggagat gatcatcgtg gatgattgct ccacctcctg
     4441 ggaacaactg caacagtacg tcaccgccct caacgatccg cgtatcactt acattcacaa
     4501 cgatattaac agcggggcgt gcgcggtacg taaccaggcg attatgctgg cgcaagggga
     4561 atatatcacc gggattgatg acgatgacga atggacaccc aaccgtctga gcgtcttcct
     4621 cgcccataaa cagcaactgg tcacacacgc ctttttgtac gctaacgact acgtatgcca
     4681 gggagaagtt tattcccaac cggcaagctt accactgtat cccaaatcgc cgtactcacg
     4741 ccgcctgttc tataaacgca atatcattgg caatcaggtc tttacctggg catggcgttt
     4801 caaagagtgt ttgttcgata ccgaactgaa agcagcacag gattacgaca tcttcctgcg
     4861 gatggtggtg gagtacggcg aaccgtggaa agtggaagag gcgacgcaga tcctgcatat
     4921 caatcacggt gaaatgcaga tcacctcgtc accgaaaaaa ttctccgggt acttccactt
     4981 ttaccgcaag cacaaagata aatttgaccg tgcaagcaaa aaatatcagc tgtttaccct
     5041 ctaccagatc cgcaataaac gtatgacctg gcgcactttg ctgacgctgc tgtcggtgcg
     5101 taacggcaaa cgtctggctg atggtattcg ggggcgctaa agatgctgga agatctgcgt
     5161 gccaacagct ggagtttacg cccgtgctgc atggttcttg cctatcgtgt tgctcatttt
     5221 tgttcggtgt ggcgcaaaaa gaacgtcctc aacaatctgt gggcggcccc gctgctggtg
     5281 ctgtatcgca ttatcaccga atgctttttc ggttatgaaa tccaggccgc cgcgaccatt
     5341 ggccgccgct ttactatcca tcacggttac gccgtagtga tcaataaaaa cgtggtagcg
     5401 ggggatgatt tcaccattcg ccacggcgtc actatcggca atcgtggtgc cgataacatg
     5461 gcatgtccac acattggcaa cggcgtcgaa ctcggtgcca acgtcattat tcttggtgat
     5521 atcacgcttg gtaacaacgt caccgtgggc gcgggcagcg tggtgctcga ctctgtcccg
     5581 gacaacgcgc tggtggtggg agaaaaagcg cgagtgaagg taattaaatg aatattctgc
     5641 aatttaatgt gcgactggcg gaaggcgggg cggcaggtgt ggcgttagat ctccatcagc
     5701 gtgcgctgca acaggggctg gcgtcacatt ttgtctacgg ctacggcaaa ggcggcaaag
     5761 agagcgtcag ccatcaaaac tatccgcagg tcgtcaaaca tacgccgcgg atgaccgcga
     5821 tggcgaacat tgcgttgttt cgtctgttta atcgcgatct gtttggcaat ttcaatgagt
     5881 tatatcgcac cattacttgc acaccgggtc cggtggtcct gcattttcat gtgctgcaca
     5941 gctactggct aaatcttaag agcgtggtgc gcttttgcga aaaggtgaaa aaccacaaac
     6001 cggatgtcac tctggtctgg acgctgcacg accactggag cgttaccggg cgctgcgcct
     6061 ttactgacgg ttgtgaaggc tggaaaacgg gctgccagaa atgcccgacc ttaaataatt
     6121 atccgccggt gaagattgat cgggcgcacc agctggtggc gggcaaacgc cagttattcc
     6181 gtgagatgct ggcgctgggc tgtcagttta tttcccccag ccagcatgtg gctgacgctt
     6241 tcaatagcct gtacggtcca gggcgttgcc ggattatcaa taacggtatt gatatggcaa
     6301 ccgaagcgat tctggcggat ctgcctccgg tgcgcgaaac ccagggtaag ccgaaaatcg
     6361 cggtggtggc gcatgacctg cgttacgacg gcaaaactaa ccagcaactg gtgcgtgaga
     6421 tgatggcgct gggcgacaaa atcgaactgc atacctttgg taagttctcg ccgttcaccg
     6481 ctggcaacgt ggttaatcac ggctttgaaa ctgacaagcg caagttgatg agcgcgctca
     6541 atcagatgga tgcgctggtg ttcagttctc gcgtcgataa ctacccgctg attttgtgtg
     6601 aggcgctatc gattggcgtg ccggtgattg ccacccatag cgacgcggcg cgggaagtgc
     6661 tgcaaaaatc cggcggtaaa accgtcagcg aagaagacgt gctgcaactg gtgcagttaa
     6721 gcaaaccgga aatcgcgcag gcgatatttg gtaccacgct ggctgagttc agccaacgca
     6781 gccgcgcggc ctacagtgga caacagatgc tggaggagta tgtcaacttc tatcagaatc
     6841 tgtagctacc tgttgttgcc gctgatttat ctgctggtta acgtcaaaat cgcccagctt
     6901 ggcgaaagtt tccccatcac catcgtcact tttttacctg tcttgttact gctgttttta
     6961 gagcgcatca gcgttaaaaa attgatgatt gccttaggga ttggtgcggg actcacggcg
     7021 tttaactatc tgtttggtca gtcgctggat gccagtaaat acgtcacttc aactatgctg
     7081 tttgtctata ttgtgatcat tattggcatg gtgtggagta ttcgttttaa aacaatttcg
     7141 ccacacaacc atcgcaagat attacgtttc ttttatctgg tggtcgggtt ggtggtggcg
     7201 ctggcggcgg tggagatggc gcaaattatc ctcaccggcg gcagcagtat tatggagtcg
     7261 atttcgaaat atctgattta cagcaacagc tatgtactga atttcattaa attcggcggc
     7321 aagcgcacga cagcacttta tttcgaaccg gcatttttcg ctctggcatt aatctcaatt
     7381 tggctcagca tcaaacagtt tggtatcaaa acgcctaaaa ccgatgctat gattctcgca
     7441 gggataatat tatccggatc gttttcaggg gttatgacct ttatcctgtt ttatttgctg
     7501 gagtgggcat ttcaatatct gaataaagag gcgattaaga aaaagttacc gttagcattg
     7561 atttctctgg ctgtattcct ggttggtgtg gtaatcgcgt ttccttatat ttccacccgt
     7621 ctgggcgatt taggtacgga aggatcgtca tcgtattatc gtattgtcgg tccgctggtg
     7681 atggtcggtt attctttgac ccatattgac ggtgtagtca gatttggctc actttatgaa
     7741 tatgtcgcat cattcggaat atttaacggt gcggatgtcg gaaaaaccat agacaatggt
     7801 ttgtatctgc tgattattta tttttcctgg ttcgcagtgt ttttatcgct gtggtacatg
     7861 gggaaagtga taaaaatgat gatcaacgct tttggtgata accgcaattt tcgcgtgcaa
     7921 ttatatcttt ttactccggt gtcgctgttt tttaccggtt cgatatttag cccggaatat
     7981 gcatttttaa tcgtctgtcc gtttattttg cgaaaagcgt taaatattac gaggtaagaa
     8041 taagaacatg ttgcttagca taatcactgt cgcgtttcgt aacctcgaag ggatagtcaa
     8101 aacacatgcc tcgctggcgc atctggcgca ggcggaagat atcagcttcg aatggattgt
     8161 tgtcgatggc ggttccaatg acggcacccg tgagtatctg gaaaatctca atggtatcta
     8221 taacctacgc tttgtcagcg agccagataa cggtatctac gacgccatga acaaaggcat
     8281 tacgatggca caaggcaagt tcgcgttgtt tctcaattcg ggcgatattt ttcatcagga
     8341 tgccgcaaat tttgtccgta agttaaaaat gcaaaaagat aacatgatga tcaccggcga
     8401 tgcgctgctg gattttggcg acgggcataa aattaaacgt agcgccaaac cgggctggta
     8461 tatttatcac agcctgcccg ccagtcatca ggcgatattt ttccctgtat ccggcttgaa
     8521 aaaatggcgt tatgacctgg aatataaagt ttcttccgac tacgcgctgg cagccaaaat
     8581 gtataaagcc ggttatgcat ttaaaaaact caatggcctg gtgtctgaat tttccatggg
     8641 tggggtatct accaccaata atatggaatt gtgtgctgac gcgaaaaaag tccaacgaca
     8701 aatattacat gtacctggct tttgggctga attatcctgg catttacgcc aacgtactac
     8761 ctcaaagacg aaagccttat ataacaaagt ctgaatataa ggagaaccaa tgcaagattt
     8821 aagcggtttc tcggtgccga aagggttccg gggcggcaac gctattaaag tgcaattatg
     8881 gtgggcagta caggcaacaa tatttgcctg gtcgccacaa gtattgtatc gctggcgggc
     8941 ttttttatta cgtttattcg gagcaaaaat aggaaaaaac gtagttattc gtccgtcagt
     9001 aaaaattacc tatccgtgga aattaacctt aggtgattac gcgtgggtcg gcgatgacgt
     9061 caatttatat accctcggtg aaataaccat tggcgcacat tcggtgatat cgcaaaaaag
     9121 ttatttatgc accggtagcc acgaccatgc aagtcaacat ttcaccatta acgccacgcc
     9181 tattgtgatt ggcgagaaat gctggctggc aaccgatgtc tttgttgccc caggcgtcac
     9241 aatcggcgac ggcaccgtcg tgggtgcacg aagcagtgtt tttaaatcgc ttccggcaaa
     9301 tgtggtttgc cgggggaatc ccgcagtggt gatacgcgaa cgcgttgaaa ctgaataaat
     9361 tcaaaaatac agaggaataa gacatgtcaa aagtcgctct catcaccggt gtaaccggac
     9421 aagacggttc ttacctggca gagtttctgc tggaaaaagg ttacgaggtg catggtatta
     9481 agcgtcgcgc atcgtcattc aacaccgagc gcgtggatca catttatcag gatccgcaca
     9541 cctgcaaccc gaaattccat ctgcattatg gcgacctgag tgatacctct aacctgacgc
     9601 gcattttgcg tgaagtacag ccggatgaag tgtacaacct gggcgcaatg agccacgttg
     9661 cggtctcttt tgagtcacca gaatataccg ctgacgtcga cgcgatgggt acgctgcgcc
     9721 tgctggaggc gatccgcttc ctcggtctgg aaaagaaaac tcgtttctat caggcttcca
     9781 cctctgaact gtacggtctg gtgcaggaaa ttccgcagaa agaaaccacg ccgttctacc
     9841 cgcgatctcc gtatgcggtc gccaaactgt acgcctactg gatcaccgtt aactaccgcg
     9901 aatcctacgg catgtacgcc tgtaacggta ttctcttcaa ccatgaatcc ccgcgccgcg
     9961 gcgaaacctt cgttacccgc aaaatcaccc gcgcaatcgc caacatcgcc caggggctgg
    10021 agtcgtgcct gtacctcggc aatatggatt ccctgcgtga ctggggccac gccaaagact
    10081 acgtaaaaat gcagtggatg atgctgcaac aggaacagcc agaagatttc gttatcgcga
    10141 ccggcgttca gtactccgta cgtcagttcg tggaaatggc ggcagcacag ctgggcatca
    10201 aactgcgctt tgaaggcacg ggcgttgaag agaagggcat tgtggtttcc gtcaccgggc
    10261 atgacgcgcc gggcgttaaa ccgggcgatg tgattatcgc cgttgacccg cgttacttcc
    10321 gtccggcgga agttgaaacg ctgctcggcg acccgaccaa agcgcacgaa aaactgggct
    10381 ggaaaccgga aatcaccctc agagagatgg tgtctgaaat ggtggctaat gacctcgaag
    10441 cggcgaaaaa acactctctg ctgaaatctc acggctacga cgtggcgatc gcgctggagt
    10501 cataagcatg agtaaacaac gaatttttat cgctggccat cgcgggatgg tcggttccgc
    10561 catcaagcga cagctcgaac agcgcggtga tgtggaactg gtattacgca cccgcgacga
    10621 gctgaacctg ctggacagcc gcgccgtgca tgatttcttt gccagcgaac gtattgacca
    10681 ggtctatctg gcggcggcga aagtgggcgg cattgttgcc aacaacacct atccggcgga
    10741 tttcatctac cagaacatga tgattgagag caacatcatt cacgccgcgc atcagaacga
    10801 cgtgaacaaa ctgctgtttc tcggatcgtc ctgcatctac ccgaaactgg caaaacagcc
    10861 gatggcagaa agcgagttgt tgcagggcac gctggagccg actaacgagc cttatgctat
    10921 tgccaaaatc gccgggatca aattgtgcga atcttacaat cgccagtacg gacgagatta
    10981 ccgttcagtc atgccgacca acctgtacgg gccacacgac aacttccacc cgagtaattc
    11041 gcatgtgatc cctgcattgc tgcgtcgctt ccacgaggcg acggcacaga atgcgccgga
    11101 cgtggtggtg tggggcagcg gtacaccgat gcgtgaattc ctgcacgtcg atgatatggc
    11161 ggcggcaagc attcatgtga tggagctggc gcacgaagtc tggctggaga acacccagcc
    11221 gatgctgtcg cacattaacg tcggcacggg cgttgactgc actatccgcg agctggcgca
    11281 aaccatcgcc aaagtggtgg gttacaaagg tcgggtggtt tttgatgcca gcaaaccgga
    11341 tggcacaccg cgcaaactgc tggatgtgat gcgcctgcat cagcttggct ggtatcacga
    11401 aatatcactg gaagcggggc ttgccagcac ttaccagtgg ttccttgaga atcaagaccg
    11461 ctttcggggg taatgatgtt tttacgtcag gaagactttg ccacggtagt gcgctccaca
    11521 ccgcttgtct ctctcgactt tattgtcgag aacagtcgcg gcgagtttct gcttggcaaa
    11581 agaaccaacc gcccggcgca gggttactgg tttgtgccgg gagggcgcgt gcagaaagac
    11641 gaaacgctgg aagccgcatt tgagcggctg acgatggcgg aactggggct gcgtctgccg
    11701 ataacagcag gccagtttta cggtgtctgg cagcactttt atgacgataa cttctccggc
    11761 acggatttca ccactcacta tgtggtgctc ggttttcgct tcagagtcgc tgaagaagat
    11821 ctgttactgc cggatgagca gcatgacgat taccgctggc tgacgccgga cgcgctgctc
    11881 gccagtgaca atgttcacgc taacagccgc gcctattttc tcgctgagaa gcgtgccgga
    11941 gtacccggat tatgaaaata ctggtctacg gcattaacta ctcgccggag ttaaccggca
    12001 tcggcaaata caccggcgag atggtggaat ggctggcggc acaaggtcat gaggtgcggg
    12061 tcattaccgc accgccttac tacccgcagt ggcaggtggg cgagaactat tccgcctggc
    12121 gctacaaacg agaagagggg gccgccacgg tgtggcgctg cccgctgtat gtgccaaaac
    12181 agccgagcac cctgaaacgc ctgttgcatc tcggcagttt tgccgtcagc agttttttcc
    12241 cgctgatggc gcaacgtcgc tggaagccgg agcgcattat cggcgtagtg ccaacgctgt
    12301 tttgcacgcc gggaatgcgt ctgctggcga aactctctgg cgcgcgtacc gtgctgcata
    12361 ttcaggatta cgaagtagat gccatgctgg ggctgggcct tgccggaaaa ggcaaaggcg
    12421 gcaaagtggc acagctggca acggcgttcg aacgtagcgg actacataac gtcgataacg
    12481 tctccacgat ttcgcgttcg atgatgaata aagccatcga aaaaggcgtg gcggcggaaa
    12541 acgtcatctt cttccccaac tggtcggaaa tcgcccgttt tcagcatgtt gcagatgccg
    12601 atgttgatgc ccttcgtaac cagcttggcc tgccggataa caaaaaaatc attctttact
    12661 ccggcaatat tggtgaaaag caggggctgg aaaacgttat tgaagctgcc gatcgcctgc
    12721 gcgatgaacc actgattttt gccattgtcg ggcagggtgg cggcaaagcg cgactggaaa
    12781 aaatggcgca gcagcgtgga ctgcacaaca tgcaattttt cccgctgcaa tcgtatgacg
    12841 ctttacccgc actgctgaag atgggcgatt gccatctggt ggtgcaaaaa cgcggcgcgg
    12901 cagatgccgt attgccgtcg aaactgacca atattctggc ggtaggcggt aacgcggtga
    12961 ttaccgcaga ggcccacaca gaactggggg agctttgcga aaccttttcg ggcattgcgg
    13021 tttgcgtaga accggaatcg gtcgaggcgc tggtggcggg gatccgtcag gcgctcctgc
    13081 tgcccaaaca caacacggtg gcacgtgaat atgccgaacg cacgctcgat aaagagaacg
    13141 tgttacgtca atttataaat gatattcggg gataattatg gcgcagtcga aactctatcc
    13201 agttgtgatg gcaggtggct ccggtagccg cttatggccg ctttcccgcg tactttaccc
    13261 caagcagttt ttatgcctga aaggcgatct caccatgctg caaaccacca tctgccgcct
    13321 gaacggcgtg gagtgcgaaa gcccggtggt gatttgcaat gagcagcacc gctttattgt
    13381 cgcggaacag ctgcgtcaac tgaacaaact caccgagaac attattctcg aaccgacagg
    13441 gcgtaacact gcacctgcca ttgcactggc ggcgctggcg gcaaaacgtc atagcccgga
    13501 gagcgacccg ttaatgctgg tattggcggc ggatcatgtg attgccgatg aagacgcgtt
    13561 ccgtgccgcc gtgcgtaatg ccatgccata tgccgaagcg ggcaagctgg tgaccttcgg
    13621 cattgtgccg gatctacctg aaaccggtta tggctatatt cgtcgcggtg aagtgtctgc
    13681 gggtgagcag gatacggtgg cctttgaagt ggcgcagttt gtcgaaaaac cgaatctgga
    13741 aaccgcccag gcctatgtgg caagcggcga atattactgg aacagcggca tgttcctgtt
    13801 ccgcgccgga cgctatctcg aagaactgaa aaaatatcgc ccggatatcc tcgatgcctg
    13861 cgaaaaagcg atgagcgccg tcgatccgga tctcgatttt attcgcgtgg atgaagaagc
    13921 gtttctcgcc tgcccggaag agtcggtgga ttacgcggtc atggaacgta cggcagatgc
    13981 cgttgtggtg ccgatggatg cgggctggag cgatgtcggt tcctggtctt cattatggga
    14041 gatcagcgcc cacaccgccg agggcaacgt ttgccacggc gatgtgatta atcacaaaac
    14101 agaaaacagc tatgtgtatg ccgaatctgg cctcgtaacc accgtcgggg tgaaagatct
    14161 ggtggtagtg cagaccaaag atgcggtgct gattgccgac cgtaacgcgg tgcaggatgt
    14221 gaaaaaagtg gtcgagcaga tcaaagccga tggtcgccat gagcatcggg tacatcgcga
    14281 agtgtatcgt ccgtggggca aatatgactc tatcgacgcg ggcgaccgct accaggtgaa
    14341 acgcatcacc gtgaaaccgg gcgaaggctt gtcggtacag atgcaccatc accgcgcgga
    14401 acactgggtg gtggtcgcgg gaacggcaaa agtcactatt gacggtgata tcaaactgct
    14461 tggtgaaaac gagtccattt atattccgct gggggcgacg cactgcctgg aaaacccggg
    14521 gaaaattccg ctcgatttaa ttgaagtgcg ctccggctct tatctcgaag aggatgatgt
    14581 ggtgcgcttc gcggatcgct acggacgggt gtaaacgtcg catcaggcgc tgtttgtcgg
    14641 atgcggcgtg aacgccttat ccgacctacg gttcggattt gtaggcctga taagacgcgg
    14701 cagcgtcgca tcaggcaatg aatgcgaaac cgcgatgtaa ataacgacaa aaacaaaatt
    14761 ggccgtttcg gtcagggcca actattgcct gaaaaagggt aacgatatga aaaaattaac
    14821 ctgctttaaa gcctatgata ttcgcgggaa attaggcgaa gaactgaatg aagatattgc
    14881 ctggcgcatt ggtcgcgcct atggcgaatt tctcaaaccg aaaaccattg tgttaggcgg
    14941 tgatgtccgc ctcaccagcg aaaccttaaa actggcgctg gcgaaaggtt tacaggatgc
    15001 gggcgtcgat gtgctggata ttggcatgtc cggcaccgaa gagatctatt tcgccacgtt
    15061 ccatctcggc gtggatggcg gcattgaagt taccgccagc cataatccga tggattataa
    15121 cggcatgaag ctggttcgcg agggggctcg cccgatcagc ggagataccg gactgcgcga
    15181 cgtccagcgt ctggctgaag ccaacgactt ccctcccgtc gatgaaacca aacgcggtcg
    15241 ctatcagcaa atcaacctgc gtgacgctta cgttgatcac ctgttcggtt atatcaacgt
    15301 caaaaacctc acgccgctca agctggtgat caactccggg aacggcgcag cgggtccggt
    15361 ggtggacgcc attgaagccc gctttaaagc cctcggcgca cccgtggaat taatcaaagt
    15421 gcacaacacg ccggacggca atttccccaa cggtattcct aacccactac tgccggaatg
    15481 ccgcgacgac acccgcaatg cggtcatcaa acacggcgcg gatatgggca ttgcctttga
    15541 tggcgatttt gaccgctgct tcctgtttga cgaaaaaggg cagtttattg agggttacta
    15601 cattgtcggc ctgctggcag aagcgttcct cgaaaaaaat cccggcgcga agatcatcca
    15661 cgatcctcgt ctctcctgga ataccgttga tgtggtgact gccgcaggcg gcaccccggt
    15721 aatgtcgaaa accggacacg cctttattaa agaacgtatg cgcaaggaag acgccatcta
    15781 cggtggcgaa atgagcgccc accattactt ccgtgatttc gcttactgcg acagcggcat
    15841 gatcccatgg ctgctggtcg ccgaactggt gtgcctgaaa ggaaaaacgc tgggcgaact
    15901 ggtgcgcgac cggatggcgg cgtttccggc aagcggtgag atcaacagca aactggcgca
    15961 acccgttgag gcgattaacc gcgtggaaca gcactttagc cgtgaggcgc tggcggtgga
    16021 tcgcaccgat ggcatcagca tgacctttgc cgactggcgc tttaatctgc gctcctccaa
    16081 caccgaaccg gtggtgcggt tgaatgtgga atcgcgcggt gatgtaccgc tgatggaagc
    16141 gcgaacgcga actctgctga cgttgctgaa cgagtaatgt cattactttc cccctcaccc
    16201 tgacgctcta ctgcctgcgg agagaagtga gcacggaact ttcttctccc cctcgcccct
    16261 ttggggagag ggccggggtg aggggcgagg cgacaataga acaggaacaa cgatgacaaa
    16321 tctaaaaaag cgcgagcgag cgaaaaccaa tgcatcgtta atctctatgg tgcaacgctt
    16381 ttcagatatc accatcatgt ttgccggact atggctggtt tgcgaagtga gcggactgtc
    16441 attcctctac atgcacctgt tggtggcgtt gattacgctg gtggtgttcc agatgctggg
    16501 cggcatcacc gatttttatc gctcatggcg cggtgttcgg gcagcgacag aatttgccct
    16561 gttgctacaa aactggacct taagcgtgat tttcagcgcc ggactggtgg cgttcaataa
    16621 tgatttcgac acgcaactga aaatctggct ggcgtggtat gggctgacca gcatcggact
    16681 ggtggtttgc cgttcatgta ttcgcattgg cgcgggctgg ctgcgtaatc atggctataa
    16741 caagcgtatg gtcgccgtag cgggggattt agccgccgga caaatgctga tggagagctt
    16801 ccgtaatcag ccgtggttag ggtttgaagt ggtgggcgtt taccacgacc cgaaaccggg
    16861 tggcgtttct aacgactggg cgggcaatct gcaacagctg gtcgaggatg cgaaagcggg
    16921 caagattcat aacgtctata tcgcgatgca aatgtgcgac ggcgcgcgag tgaaaaaact
    16981 ggtccatcaa ctggcggaca ctacctgttc ggtgctgctg atccccgatg tctttacctt
    17041 caacattctc cattcccgta ttgaagagat gaacggcgta ccagtggtgc cgctgtatga
    17101 cacgccgctt tccggggtta accgcctgct caaacgtgca gaagacattg tgctggcaac
    17161 gctgattctg ctgctgatct ccccggtgct gtgctgcatt gcgttggcgg tgaaactgag
    17221 ctcaccgggg ccggttattt tccgccagac tcgctacggc atggacggca agccgatcaa
    17281 agtgtggaag ttccgttcga tgaaagtgat ggagaacgac aaagtggtga ctcaggcgac
    17341 gcagaacgat ccgcgcgtca ccaaagtggg gaactttctg cgccgcacct cgctggacga
    17401 attgccgcag tttatcaatg tgctgaccgg ggggatgtcg attgtcggtc cacgtccgca
    17461 cgcagtagcg cataacgaac agtatcgcca gctcattgaa ggctacatgc tgcgccacaa
    17521 agtgaaaccg ggcattaccg gctgggcgca gattaatggc tggcgcggcg aaaccgacac
    17581 gctggagaaa atggaaaaac gcgtcgagtt cgaccttgag tacatccgcg aatggagcgt
    17641 ctggttcgat atcaaaatcg ttttcctgac ggtattcaaa ggcttcgtta acaaagcggc
    17701 atattgatat gagcttacgt gaaaaaacca tcagcggcgc gaagtggtcg gcgattgcca
    17761 cggtgatcat catcggcctc gggctggtgc aaatgactgt gctggcgcgg attatcgaca
    17821 accaccagtt cggtttgctg actgtgtcgc tggtgattat cgcgctggca gatacgcttt
    17881 ctgacttcgg tatcgctaac tccattattc agcgaaaaga aatcagccac cttgaactca
    17941 ccacgttgta ctggctgaac gtcgggctgg ggatcgtggc gtgtgtggcg gtatttttgt
    18001 tgagtgatgc cattggcgac atgctgaata acccggacct ggcaccgttg attaaaacat
    18061 tgtcactagc gtttgtggtg atcccccacg ggcaacagtt ccgcgcgttg atgcaaaaag
    18121 agctggagtt caacaaaatc ggcatgatcg aaaccagcgc ggtgctggcg ggctttactt
    18181 ttacggtggt tagcgcccat ttctggccgc tggcgatgac cgcgatcctc ggttatctgg
    18241 tcaatagtgc ggtgagaacg ctgctgtttg gctactttgg ccgcaaaatc tatcgccccg
    18301 ctctgcattt ctcgctggcg tcggtggcac cgaacttacg ctttggtgcc tggctgacgg
    18361 cggacagcat catcaactat ctcaacacca acctttcaac actcgtgctg gcgcgtattc
    18421 tcggcgcggg cgtggctggg ggatacaacc tggcgtacaa cgtggccgtt gtgccgccga
    18481 tgaaactgaa cccgatcatc acccgcgtgt tgtttccggc gtttgccaaa attcaggacg
    18541 ataccgaaaa gctgcgcgtc aacttctaca agctgctgtc ggtagtgggg attatcaact
    18601 ttccggcgct gctggggctg atggtggtgt cgaataactt tgtaccgctg gtatttggcg
    18661 aaaagtggag cagcattatt ccggtgctac agctgctgtg tgtggtgggt ctgctgcgct
    18721 ccgtgggtaa cccgattggt tcgctgctga tggcgaaagc gcgggtcgat atcagcttta
    18781 aattcaacgt atttaaaaca tttctgttta ttccggcgat tgttattggt gggcagatgg
    18841 cgggcgcaat cggcgtcacg cttggcttcc tgctggtgca gattatcaac actattctga
    18901 gctatttcgt gatgattaaa ccggttcttg gttccagtta tcgccagtac atcctgagtt
    18961 tgtggctgcc gttttatctc tcgctgccaa cgctggtggt cagttatgcg ctgggcattg
    19021 tgctgaaagg gcaactggcg ctggggatgc tgctggcggt gcaaatagcc gcgggtgtgc
    19081 tggcgtttgt ggtgatgatt gtgctgtcgc gccatccgct ggtggtggaa gtgaagcgtc
    19141 agttttgtcg cagcgaaaaa atgaaaatgc ttttacgggc ggggtgagtt gttgttttcg
    19201 gttccgacaa cggttgccgg gtgcagcgta aaacgcctta tccgtcctac ggttctgtgc
    19261 tcgcatcagg caaaaaacgg caacgaacac aacaaacaca gcctcacctg gcatcaaatt
    19321 tatgaggaaa aaatgaaatt acttattctg ggcaaccaca cttgcggcaa tcgtggcgac
    19381 agcgccatct tgcgcggctt acttgatgcc atcaacatcc tcaatccaca tgccgaagtg
    19441 gacgtgatga gccgctatcc tgtcagttct tcctggctac tcaaccgccc ggtaatgggc
    19501 gatccgctgt tcctgcaaat gaaacaacac aacagcgcgg cgggcgttgt cgggcgcgtt
    19561 aaaaaagtcc tccgtcgccg ctaccagcat caggtattgc tctcacgcgt caccgacagt
    19621 ggcaagctgc gtaatatcgc catcgcccag ggattcaccg acttcgtgcg cctgctgtca
    19681 ggttacgacg ccattattca ggtcggcgga tcgttttttg tcgatctcta cggcgtgcca
    19741 cagtttgaac atgcactttg cacgtttatg gcgaaaaagt cgctgtttat gattggtcac
    19801 agtgttggcc cgttccagga tgagcaattt aaccaactgg cgaactacgt ttttggtcac
    19861 tgcgacgcgc tgatcctgcg cgaatcggtc agccttgaac tgatgaaacg cagcaatatc
    19921 accacttcaa aagttgaaca tggcgtcgat accgcgtggc tggtcgatca ccacacagaa
    19981 gacttcaccg ccagctatgc tgttcaacac tggctggacg ttgccgcaca acagaaaacg
    20041 gtggcaatta ccctgcgcga actggcaccg ttcgacaaac gtctcggcac cactcaacaa
    20101 gcatatgaaa aagcctttgc cggggtggtc aatcgcattc tcgacgaagg ctatcaggtg
    20161 attgcgctct ccacctgtac gggtatcgac agctataaca aagacgaccg catggtggcg
    20221 ctcaacctgc gccagcacat cagcgatcct gcccgttacc acgtagtgat ggatgaactc
    20281 aacgatctgg aaatgggcaa aattctgggg gcctgtgaac tcaccatcgg tacgcgcctg
    20341 cactccgcca ttatctcaat gaactttgcc accccggcga ttgccatcaa ttatgaacat
    20401 aaatccgccg ggattatgca gcagctggga ctaccggaga tggcgattga tatccgtcat
    20461 ttattagacg gcagcctgca agtgatggtt gcggatacct taggccagct tccggcactg
    20521 aacacgcgac ttaacgaagc cgtcagtcgc gaacgtcaga cgggaatgca gatggtgaaa
    20581 tctgtgcttg aacacatcag ggaggcgaaa tgaaggtcgg cttcttttta ctgaaatttc
    20641 cgctgtcgtc ggaaaccttc gtcctcaatc aaattaccgc gtttattgat atgggctttg
    20701 aggtggagat tgtcgcgctg caaaaaggcg acacccagaa cacccacgcg gcatggacga
    20761 aatacaacct tgccgccaga acccgttggt tacaggacga acctacgggc aaagtggcga
    20821 aactgcgcca ccgtgccagc cagatcttac gcggcattca tcgtaaaaat acctggcagg
    20881 cacttaacct caaacgctat ggtgccgagt cgcgaaacct gattttgtct gccatttgcg
    20941 gccaggtcgc aacaccattt tatgccgatg tctttatcgc tcattttggc cctgcggggg
    21001 taaccgcagc aaaactccgc gaactgggtg tcattcgcgg caaaattgcc actatcttcc
    21061 acggcattga tatctccagt cgggaggtgc tcaaccatta cactcccgaa tatcagcaac
    21121 tgttccgccg tggcgatctg atgctaccaa taagcgatct gtgggccgga aggttgcaaa
    21181 aaatgggctg cccgagggaa aaaatcgccg tatcgcgcat gggcgtggac atgacgcgtt
    21241 ttagcccgcg tccggtgaaa gcgcccgcaa cgccgctgga aatcatttcc gtcgcacgct
    21301 taaccgagaa aaaaggcctg catgtggcga ttgaagcctg ccgtcagttg aaagagcagg
    21361 gcgtggcatt tcgctatcgc atcctcggca ttggcccgtg ggaacgacgc ctgcgcaccc
    21421 tcatcgaaca atatcaactg gaagatgtgg tagagatgcc gggctttaaa ccgagccacg
    21481 aagtgaaagc gatgctcgac gacgcggatg tcttcctgtt gccatcggtt acaggtgcgg
    21541 atggcgatat ggaaggtatt ccggtggcac taatggaagc gatggcggtc ggcattccgg
    21601 tggtttctac tctgcatagc ggaataccgg aactggtgga ggccgataaa tccggctggc
    21661 tggtgcctga gaacgatgct cgcgcactgg cgcaacgact ggcggcgttt agccaactgg
    21721 acaccgacga actggctccg gtcgtcaaac gtgcgcgcga aaaagtcgaa cacgatttta
    21781 accagcaggt gatcaatcga gaactcgcca gcttgctgca ggctttatag aggagaacgc
    21841 atgccattta aaaaactctc ccgacgcacc ttcctgacgg caagctcggt gcttgccttc
    21901 ctccataccc ctttcgctcg cgcgcttccc gcccgacaaa gcgttaacat taacgactac
    21961 aaccctcgcg actggatcgc ctcatttaaa caagccttca gcgaagggca aacggtcgtc
    22021 gtgcctgccg gattggtttg cctcaatatc aacaccggca tctttatccc tcctggcaaa
    22081 acgttacaca tccttggaag cctgcgcggc aacggcagag ggcgatttgt cttacaggac
    22141 ggcagccagg tgacagggga ggagggcggc agtatgcata acatcaccct ggatgtgcgc
    22201 ggctctgact gcaccatcaa agggctggcg atgagcggct ttggtccggt aacgcagatt
    22261 tatatcggcg gcaaaaacaa acgggtcatg cgtaacctga ccatcgataa cctgaccgtt
    22321 agccacgcta attacgccat cttacgccag ggatttcata accagattat cggtgccaac
    22381 atcaccaatt gtaagttcag cgacttacag ggcgacgcca ttgaatggaa tgtggcgatt
    22441 aacgaccgtg atattttgat atctgaccat gtcatcgagc gcatcaactg tactaacggc
    22501 aaaatcaatt ggggcatcgg cataggtctt gcgggaagca cttatgataa caactatccg
    22561 gaagaccagg cagtgaaaaa ctttgtcgtg gcgaacatca cgggatcgga ttgtcggcag
    22621 ttgatacatg ttgaaaatgg taaacatttt gttattcgta atatcaaagc ctgcaatatc
    22681 acgccggatt tcagtaagaa agcaggtatt gataacgcca cggtcgctat ttacggttgt
    22741 gacaatttcg tgattgataa tattgaaatg attaatagcg ccgggatgtt aatcggctat
    22801 ggggtaatta aaggcaaata tctctcgata ccgcagaatt tccgagtgaa taatattcag
    22861 ctggataata cccaccttgc ttacaaattg cgcggcatcc aaatctccgc cgggaatgct
    22921 gtctcctttg tggcgctgac taacgttgag atgaagcgtg cgtcgctgga gttacacaac
    22981 aagccgcaac acctttttat gcgtaatatc aaagtgatgc aggaatcctc tgttggaccc
    23041 gcattgagca tgaacttcga catgcgcaaa gacgttcgcg gcgtgtttat ggcgaaaaaa
    23101 gaaacactgc tatctcttgc aaatgttcat gcggtgaatg aaagagggca aagctccgtc
    23161 gatatcgaca ggataaatca ccatattgtt aatgtggaaa agattaactt tagattgccg
    23221 gaacggaggg agtagatttg cgaccattcc tggaaaaatg gagccatact taggaacaat
    23281 gctactgcaa tctacaacga agcggcgtaa catcacaagt aattcagtaa tcaattcagg
    23341 gtaattgatg ctggcgaaaa aaatcgaaca agctataatt cagcaaccat tttacaggtg
    23401 gatgaaataa tgacgaattt aaaagcagtt attcctgtag cgggtcttgg gatgcatatg
    23461 ttgcctgcca ctaaggcgat tcccaaagag atgctaccga tcgtcgacaa gccaatgatt
    23521 cagtacattg ttgacgagat tgtggctgca gggatcaaag aaatcctcct ggtaactcat
    23581 gcgtccaaga acgcggtcga aaaccacttc gacacctctt atgaattaga atctcttctt
    23641 gaactgcgcg tgaagcgtca actgctggcg gaagtacagt ccatctgtcc gccaggcgtg
    23701 accattatga acgtgcgtca gggcgaacct ttaggtttag gccactccat tttgtgtgca
    23761 cgacccgcca ttggtgacaa tccatttgtc gtggtactgc cggacgttgt gatcgacgac
    23821 gccagtgccg acccgctgcg ctacaacctt gctgccatga ttgcgcgttt caatgaaacg
    23881 ggccgtagcc aggtgctggc aaaacgtatg ccgggtgagc tctctgaata ctctgtcatc
    23941 cagaccaaag aaccgctgga tcgcgaaggt aaagtcagcc gcattgttga atttatcgaa
    24001 aaaccggatc agccacaaac gctggactca gacatcatgg ccgttggtcg ctatgtactt
    24061 tctgccgata tttggccgga actggaacgt actcagcctg gtgcatgggg acgtattcaa
    24121 ctgactgatg ctattgccga gctggcgaaa aaacagtccg ttgatgccat gctgatgact
    24181 ggggacagtt acgactgcgg taaaaaaatg ggctatatgc aggcgttcgt gaagtatgga
    24241 ttacgaaacc tgaaagaagg ggcgaagttc cgtaaaggca ttgagaagct gttaagcaaa
    24301 taatgaaaat ctgaccggat gtaacggttg ataagaaaat tataacggca gtgaagattc
    24361 gtgatgaaag taatttgttg cgaatattcc tgccgttgtt ttatataaac aatcagaata
    24421 acaacgagtt agcaatagga ttttagtcaa agttttccag gattttcctt gtttccagag
    24481 cggattggta agacaattag cgtttgaatt tttcgggttt agcgcgagtg ggtaacgctc
    24541 gtcacatcgt agacatgcat gcagtgcact ggtagctgta aagccagggg cggtagcgtg
    24601 cattaatacc cctattaatc aacctaagag ccgcttattt cacagcatgc tctgaagtaa
    24661 tatggaataa taaagtgaag atacttgtta ctggtggcgc aggatttatt ggttctgctg
    24721 tagttcgtca cattataaat aatacgcagg atagtgttgt taatgtcgat aaattaacgt
    24781 acgccggaaa cctggaatca cttgctgatg tttctgattc tgaacgctat gtttttgaac
    24841 atgcggatat ttgcgatgca gctgcaatgg cgcggatttt tgctcagcat cagccggatg
    24901 cagtgatgca cctggctgct gaaagccatg ttgaccgttc aattaccggc cctgcggcat
    24961 ttattgaaac caatattgtt ggtacttatg tccttttgga agccgctcgc aattactggt
    25021 ctgctcttga tagcgacaag aaaaatagct tccgttttca tcatatttct actgacgaag
    25081 tctatggtga tttgcctcat cctgacgaag taaataatac agaagaatta cccttattta
    25141 ctgagacgac agcttacgcg ccaagcagtc cttattccgc atccaaagca tctagcgatc
    25201 atttagtccg cgcgtggaaa cgtacctatg gtttaccgac cattgtgact aactgttcga
    25261 ataactacgg cccttatcac tttccggaaa aattgattcc gctggtaatt cttaatgctc
    25321 tggaaggtaa ggcattacct atttatggca aaggggatca aattcgcgat tggctatatg
    25381 ttgaagatca tgcgcgtgcg ttatataccg ttgtcaccga aggtaaagcg ggtgaaactt
    25441 ataacattgg cggacacaac gaaaagaaaa acatcgatgt tgtgctgact atttgtgatt
    25501 tgttggatga gattgtaccg aaagagcaat cttatcgtga gcaaattact tatgttgccg
    25561 atcgtccggg acacgatcgc cgttatgcga ttgatgctga gaagattagc cgcgaattgg
    25621 gctggaaacc gcaggaaacg tttgagagcg ggattcggaa gacagtggaa tggtacctgt
    25681 ccaatacaaa atgggtcgaa aatgtgaaaa gtggtgccta tcagtcatgg attgcacaga
    25741 actatgaggg ccgtcagtaa tgaatatcct ccttttcggc aaaacagggc aggtaggttg
    25801 ggaactacag cgtgctctgg cacctctggg taatttgatt gctcttgatg ttcactctac
    25861 tgattattgc ggtgatttta gtaatcctga aggtgtagct gaaaccgtaa gaagcattcg
    25921 gcctgatatt attgtcaacg cagccgctca caccgcagta gacaaagcag aatcagaacc
    25981 ggagtttgca caattactta acgcaacaag tgtcgaagcg attgcgaaag cagccaatga
    26041 agtcggcgcc tggcttattc attactcgac tgattacgtc ttccctggaa atggcgatat
    26101 gccatggcgg gagacggatg caaccgcacc actaaatgtt tacggtgaaa ccaagttagc
    26161 cggagaaaaa gcgttacagg aatattgcgc aaagcatctt attttccgga ccagctgggt
    26221 ctatgcagga aaaggaaata acttcgccaa aacgatgtta cgtctggcaa aagagcgtga
    26281 agaattagcg gttattaatg atcagtttgg tgcgccaaca ggtgctgaac tgctggctga
    26341 ttgtacagca catgccattc gtgtcgcact gaataaacca gaagtcgcag gcttgtacca
    26401 tctggtagcc agtggtacca caacctggca cgattatgct gcgctggttt ttgaagaggc
    26461 acgcaaagca ggtattcccc ttgcactcaa caagctcaac gcagtaccaa caacagccta
    26521 tcctacacca gctcgtcgtc cgcataactc tcgccttaat acagaaaaat ttcagcagaa
    26581 atttgcgctt gttttgcctg attggcaggt tggcgtgaaa cgaatgctca acgaattatt
    26641 tacgactaca gcaatttaat agtttttgca tcttgttcgt gatggtggag caagatgaat
    26701 taaaaggaat gatgaaatga aaacgcgtaa aggtattatt ttagctggtg gttcgggtac
    26761 tcgtctttat cctgtaacta tggctgtcag taaacagttg ttaccgattt atgataaacc
    26821 gatgatctat tacccgttgt ctacactgat gttagcgggt cttcgcgata ttctgattat
    26881 tagtacgcca caggatactc ctcgttttca acaactgctg ggtgacggga gccagtgggg
    26941 gctaaatctt cagtacaaag tgcaaccgag tccagatggt cttgcgcagg catttatcat
    27001 cggtgaagag tttatcggtg gtgatgattg tgctttggtt ctaggtgata atatctttta
    27061 cggtcacgat ctgccgaagt taatggatgt cgctgttaac aaagaaagtg gtgcaacggt
    27121 atttgcctat cacgttaatg atcctgaacg ctacggtgtc gttgagtttg ataaaaacgg
    27181 tacggcgatc agcctggaag aaaaaccgct acaaccaaaa agtaattatg cggtaaccgg
    27241 gctttatttt tatgataact acgttgtcga aatggcgaaa aatcttaagc cttctgcccg
    27301 cggtgaactg gaaattaccg atattaaccg tatttatatg gaacaggggc gtttatccgt
    27361 tgccatgatg ggacgtggtt atgcatggct ggacacgggg acacatcaaa gtcttattga
    27421 ggcaagcaat tttatcgcaa caatagaaga acgtcagggg ctgaaagttt cctgcccgga
    27481 agaaattgct taccgtaaag ggtttatcga tgctgagcag gtgaaagtat tagctgaacc
    27541 gttgaagaaa aatgcttatg gtcagtatct gctgaaaatg attaaaggtt attaataaaa
    27601 tgaacgtaat taaaacagaa attccagatg tactgatttt tgaaccgaaa gtttttggtg
    27661 atgagcgtgg tttctttatg gaaagcttta atcagaaagt ttttgaagaa gctgtagggc
    27721 ggaaggttga atttgttcag gataatcatt ctaaatcatc taagggtgtg ttacgcggac
    27781 tgcactatca gctggaaccc tatgctcaag gtaaattagt tcgttgtgtg gtcggtgaag
    27841 tatttgatgt agcagttgat attcgtaaat cgtcacctac atttgggaaa tgggttgggg
    27901 tgaatttgtc tgctgagaat aagcgtcagt tgtggatccc tgaggggttt gcgcatggtt
    27961 ttttggtgtt aagcgagata gcagagtttg tttataagac gacaaattat tatcatcctg
    28021 aatcagaagg ttgcataaaa tgggatgatt cttttttgat gattgattgg ccaaataaac
    28081 cgatagcaat ctcagaaaaa gataaaaaag gctcatcaat tttgaggcta aatgactatt
    28141 gatgttgaaa aaaaacttca aaaaataaag gaatatcatt cagtattgga gttggcaata
    28201 attcagggtg cgaatgccat atttcctgtg ttggtattcc cattttttct tattacctta
    28261 ggggaaaaca tcttttcaag tattgctgtt ggtgaagtac tagcactata tgtgcttata
    28321 ttttcgctat acagttttga tattataagt gtgcagaagg taatttcaag tgtgacaaaa
    28381 gatgaaatat ttaaagttta ca
//
LOCUS       DAZGLJ010000037        28314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000037 DAZGLJ010000000
VERSION     DAZGLJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.037"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(70..1173)
                     /gene="gldA"
                     /locus_tag="ACHK49_001475"
     CDS             complement(70..1173)
                     /gene="gldA"
                     /locus_tag="ACHK49_001475"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO9262146.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(1184..1846)
                     /gene="fsa"
                     /locus_tag="ACHK49_001476"
     CDS             complement(1184..1846)
                     /gene="fsa"
                     /locus_tag="ACHK49_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO9262147.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAISDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(1858..4359)
                     /gene="ptsP"
                     /locus_tag="ACHK49_001477"
                     /pseudo
     CDS             complement(1858..4359)
                     /gene="ptsP"
                     /locus_tag="ACHK49_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            4668..5747
                     /gene="frwC"
                     /locus_tag="ACHK49_001478"
     CDS             4668..5747
                     /gene="frwC"
                     /locus_tag="ACHK49_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO9262148.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            5762..6082
                     /gene="frwB"
                     /locus_tag="ACHK49_001479"
     CDS             5762..6082
                     /gene="frwB"
                     /locus_tag="ACHK49_001479"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO9262149.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            6133..8430
                     /gene="pflD"
                     /locus_tag="ACHK49_001480"
     CDS             6133..8430
                     /gene="pflD"
                     /locus_tag="ACHK49_001480"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO9262150.1"
                     /translation="MTNRISRLKTALFSNTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            8396..9274
                     /gene="pflC"
                     /locus_tag="ACHK49_001481"
     CDS             8396..9274
                     /gene="pflC"
                     /locus_tag="ACHK49_001481"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO9262151.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDVPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            9276..9617
                     /gene="frwD"
                     /locus_tag="ACHK49_001482"
     CDS             9276..9617
                     /gene="frwD"
                     /locus_tag="ACHK49_001482"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO9262152.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(9604..10455)
                     /gene="yijO"
                     /locus_tag="ACHK49_001483"
     CDS             complement(9604..10455)
                     /gene="yijO"
                     /locus_tag="ACHK49_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO9262153.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10604..12337)
                     /gene="eptC"
                     /locus_tag="ACHK49_001484"
     CDS             complement(10604..12337)
                     /gene="eptC"
                     /locus_tag="ACHK49_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO9262154.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLHPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(12520..15171)
                     /gene="ppc"
                     /locus_tag="ACHK49_001485"
     CDS             complement(12520..15171)
                     /gene="ppc"
                     /locus_tag="ACHK49_001485"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO9262155.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(15523..16674)
                     /gene="argE"
                     /locus_tag="ACHK49_001486"
     CDS             complement(15523..16674)
                     /gene="argE"
                     /locus_tag="ACHK49_001486"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO9262156.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            16828..17832
                     /gene="argC"
                     /locus_tag="ACHK49_001487"
     CDS             16828..17832
                     /gene="argC"
                     /locus_tag="ACHK49_001487"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO9262157.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPCCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            17840..18616
                     /gene="argB"
                     /locus_tag="ACHK49_001488"
     CDS             17840..18616
                     /gene="argB"
                     /locus_tag="ACHK49_001488"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO9262158.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            18677..20050
                     /gene="argH"
                     /locus_tag="ACHK49_001489"
     CDS             18677..20050
                     /gene="argH"
                     /locus_tag="ACHK49_001489"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO9262159.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVGELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            20317..21234
                     /gene="oxyR"
                     /locus_tag="ACHK49_001490"
     CDS             20317..21234
                     /gene="oxyR"
                     /locus_tag="ACHK49_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO9262160.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(21217..22617)
                     /gene="sthA"
                     /locus_tag="ACHK49_001491"
     CDS             complement(21217..22617)
                     /gene="sthA"
                     /locus_tag="ACHK49_001491"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO9262161.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            22948..23598
                     /gene="fabR"
                     /locus_tag="ACHK49_001492"
     CDS             22948..23598
                     /gene="fabR"
                     /locus_tag="ACHK49_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO9262162.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFTSLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            23598..23957
                     /gene="yijD"
                     /locus_tag="ACHK49_001493"
     CDS             23598..23957
                     /gene="yijD"
                     /locus_tag="ACHK49_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO9262163.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(23997..25097)
                     /gene="trmA"
                     /locus_tag="ACHK49_001494"
     CDS             complement(23997..25097)
                     /gene="trmA"
                     /locus_tag="ACHK49_001494"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO9262164.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      25211..25401
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            25466..27310
                     /gene="btuB"
                     /locus_tag="ACHK49_001495"
     CDS             25466..27310
                     /gene="btuB"
                     /locus_tag="ACHK49_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO9262165.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            27255..28112
                     /gene="murI"
                     /locus_tag="ACHK49_001496"
     CDS             27255..28112
                     /gene="murI"
                     /locus_tag="ACHK49_001496"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO9262166.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         6470 a         7120 c         7806 g         6918 t
ORIGIN      
        1 actgataagc gtagcgcatc aggctatttt gcgttcaaac tcccggacaa gccgggagtt
       61 tggagtaggt tattcccact cttgcaggaa acgctgaccg tactggtcgg ctaccagcag
      121 agcggcgtaa acctgatctg gcgtcgcgcc gccaggcatg ttgtggatgg tttcaccttc
      181 tgcacatgcc gcttctgcca caattcgcat tttcgccggg acatcttctt taatatccag
      241 ttgagcgaga gttattggca aacctaccgc atggctaagt gcagctacgg tttcgatttc
      301 ctcaaccggc gcgttttcca gtaccagctg cgtcagcgta ccgaatgcca ctttttcacc
      361 gtgataatag tgatgcgcgt ccgggatagc ggtcaggccg ttatgcaccg catgagccgc
      421 cgccagtccg ccgctttcaa aaccaacgcc gcttaaatag gtgttcgctt caatcacgcg
      481 ttccagcgca ggggttacta catgctgttc ggcggcaagc atcgcttttt cgccttcttc
      541 cagcagagtg ttgtagcaca gttctgccag tgccagcgcc gcctgggtgc acttgccgcc
      601 cgccatagtg gtagcgccgc tacgggaaca ggcgcgtgct tcaaaccagg ttgccagcgc
      661 atcgccgata cccgccgcta acagacgtgc aggagcgccc gcgacgattt tggtgtcgac
      721 aatgaccata ttcgggttat ttggcaacag tagatagcgg tcaaactcac cctcatcggt
      781 gtagataaca gacaatgcgc tgcacggtgc atcggtagag gcgatagtcg gtgcgatcgc
      841 taccggaaca cccatgaaat gtgccagtgc tttggcagta tcgagggttt ttccgccacc
      901 gataccgaga attgcgccac actgcgcagt ctccgcgatg ccacgcagac gatcgatctc
      961 attttgcgaa cattcaccgc caaacggcgc aatttctact accagtccag catctttaaa
     1021 gcttttctcg acagtggatt gagcaaaacc taaaacaaat ttgtcaccca ccactaacca
     1081 gcgttctgcc agcggcttca ggtattcgcc cagacgatta atcacatcag cgccctggat
     1141 gtatttaccc ggtgattgaa taatgcggtc cataattgct cctttagaga tgagtagtgc
     1201 caaatgcggc attccagtcg tgttcgaact tctctatagc tgactctacc gcaggggtgt
     1261 tgagcatttg ttgcgctaca tctaaaggca gggtgatgga ctcacatcct gccagcaaac
     1321 aatccagtgc ctgacgcggc gttttaaagc tggctgcaag caccatgctt tctggcgcgt
     1381 gcatttctaa cagcgcttgc agctcctgaa ccgtacgaat gccgtccccg ccctgggcat
     1441 ctacgcggtt aacatacgga gcaacgtatt ttgcccctgc cagcgcggcc agtaaccctt
     1501 gtgcagcact gtacactgcg gtgccaagcg tagtaatacc ctcttttttc agcatcttaa
     1561 tggcggccag accttcggaa gtcaccggga ttttcaccac aatgcccgga atggcgtcgc
     1621 gcaggcgctt cgcttcttcc accataccct gcgcgtcacg actcatagtc tgagcaaaca
     1681 gaatgccctc atcactaatc gctttttgca gacgcggcag aacttcccat atggactcct
     1741 tgctggcagc gataatgctc gggttagttg tcaccccggc aatggggaat atgcgtgcca
     1801 gacgttcgac ttctgcgacg ttagcggtgt ccagatacag ttccatgatg tttcctttta
     1861 cagttccagt tcatgttgca gcaggctggc gatagcgtcg gcagaggcgg cgttaaccag
     1921 cgcgttacgg aattcttcgt gcatgatgcg gcgagcgagg cgcgagaaaa tgcgcatgtg
     1981 ctgatcgccc gcagcgtgtt tgttcagggt taacataatg atgaattgcg cttcatcatc
     2041 gccccagcgc accggcgctt gcagacgcgc tacactgatg gtggattgct caatgtgttc
     2101 tgatttgctg tggggaatgg caaaactaaa gcccagaccg gtagagaaaa cggcctcgcg
     2161 cgcccacaag tcggcttcca gtttacgcgg atagcggcag cggcccgcca gcagcaggtt
     2221 atcggtcatg cctttgagca cttcttcttt gctgcgtcag tcgctttcca gcgtgatgca
     2281 ctcggcggtg accagcggtg cgtcctgttg ggtcatgcgg aattgcgcca gcaggtgttc
     2341 cacttccagc gaagtacggc aggccattgc ctggttgagc aactggcggc attcgcggct
     2401 atcaagttgc gccattcgcg ctttcgccgc cggaattgac ggtgcgctca tgctcagctc
     2461 atccagccct aaaccgacca gcaacggcag cacggaacct ttcgctccca gctcaccgca
     2521 cagaccaatc catttgccct ggcgatgcac cgcttgcacg gcgtaatcga gcgcccgcaa
     2581 gaatgccgga ttcaggctgt tgtagtgacg agtaacctta gcattatcgc gatccaccgc
     2641 cagcagatat tgcgtcaggt cattactacc aatactaaag aaatcaatct cttcgcagca
     2701 ttgatcgatg atgaacatca ccgacggcac ttccagcata atgccgagct ggatcttctc
     2761 atcaaacgga atgtgttcgt tacgcagttg ctgtttggct tccgccagtt tttctttcac
     2821 ccataagatc tcttccattg aagagatcat cgggatcatg attttcaggc tgccgtgagc
     2881 ggaggcgcgc aggatcgacc gtagttgtgt ggtgaacagc gaggcgtatt cttcataaat
     2941 acgcacagcg cgataaccga ggaacgggtt agcttctgcc ggaatgttca gataatcaac
     3001 gggtttgtcg ccgccaatgt ccatagtgcg cacaataatg ctgcgtccgt tggcagactc
     3061 cagcgcctga caaaaaatgt tgtacagctc gctttcgccc ggtgcactgg tgcgatccat
     3121 atagagcatt tcagtgcgga acaaaccaac gccttccgca ccattgccga atgcggcctg
     3181 cgcttccacg gagtgagcga tgttagcggc aatttcaatg cggataccgt cagcggtacg
     3241 ggcttgttgg gtcagccaga cacgctgttg ctcacgcagg gcatcctgta cgcgggcttc
     3301 ttgctgataa taacgggcta cggcttcccc tggctcaacc acaatcgctc cggcgttgcc
     3361 gtcgatataa atcgtttgat gttgccacgg ggtaagggca tcaatatcca caccaaccag
     3421 cgtcggaatg ttgaacgaac gggcaaggat caccgtatgt gaggtggtgc cgccgctttt
     3481 gagcaacaat cctttgaggt gatttttatc cagttcgagg aactggctgg gggtcagttc
     3541 atcagccata caaatggcgg gctgcgtcag tttgcccggt gccgggaagc gttgctcacc
     3601 gtagatttgc tggagtaact ggaagcagac gtcgcgtacg tccagggcac gttcttgcag
     3661 gtagctgctg ctggaacggg aaaactcttc acaaaagtga ttcgcactgg cgacaatcgc
     3721 ttcggcgcag cttaatccgg cgctgacgcc tgccagtaaa tgttcgtgca gggaagtatc
     3781 gccagccagg gatcggtgcg cttccagaat cgcgctggtg gcaccgtcgc tatccagcag
     3841 acgaaactca atgtttttca gtaccagcgt caggccgttt tccagtgcgg attgctcggc
     3901 gtcaacgtct ttggctgcgg gaagattact cagcgcattg agatctaaag aagagatcgg
     3961 cgtcagaatg ccgcctgcgc taccgctgca cacggtgcgg gcgcggataa tttgcggatt
     4021 cagattggtc agtgaaaccg gcagtggttc cagttcgtca gatttaactt ccgccagcgg
     4081 tgcgtcgcag tgggggaatt catcgcgcag ccattggctt aaacgctggt gcgcttcctg
     4141 ttcgtcggcc ccggaaatca gtaaccggca gttatcgccc gccagcgtgt cggtgccaat
     4201 cagcgcaagg gcgcttttgg cgttgccctt gcggtcagtg cgcaggttat gccactcaat
     4261 ttgtgatgaa aaagtattac acagcgtttc aacgtggctt gccggacgtg catgtacgcc
     4321 gttaggtagc tcacaaataa attccacaat cagggccatt gcctctctcc cggtacggtt
     4381 ttctgtttac aggataagag cgcacggcaa cggcctgcca tgtgacaaat ctgccaaaag
     4441 ctggacaaat gtaatgtaac cgtcaatttg cgacgcgtct cacaagacgc cgttttgcgg
     4501 catgcttccg gattatcgca agttatgagg cggatcgcat ttttgtactg atattacaaa
     4561 agtccagtaa atggcctttt tatccactgt ttgctccgcc tgcgattgcc tattgtttcc
     4621 tccatcaaaa atatggacat gggctttcgc aagcccggga gcagcttatg aatgagttgg
     4681 tgcagatcct gaaaaatacc cgtcagcatt taatgacggg cgtttcacac atgattccct
     4741 tcgtggtatc gggcggtatt ttgctggcgg tttccgtcat gttgtatggc aaaggcgcag
     4801 tgccggatgc cgtagccgat ccgaatctga aaaaactgtt tgatatcgga gttgcaggtt
     4861 tgacgctgat ggtgcctttc ctcgccgctt acatcggtta ctccattgca gagcgttctg
     4921 cgctggcccc gtgcgctatc ggtgcctggg ttggtaacag ctttggtgcg ggcttctttg
     4981 gtgcgctgat cgccgggatt atcggcggca tcgtggtgca ttacctgaag aaaattccgg
     5041 tgcataaagt tctgcgctcg gtgatgccta tcttcattat tcctatcgtc ggcacactga
     5101 ttaccgcagg catcatgatg tggggcttgg gcgagcctgt aggggcgttg accaacagcc
     5161 tgactcagtg gcttcagggg atgcagcagg gcagcattgt tatgctggcg gtgatcatgg
     5221 gtctgatgct ggcgttcgat atgggcggtc cggttaacaa agtggcctat gccttcatgc
     5281 tgatttgcgt tgctcagggt gtttataccg tggtggccat tgctgccgtg ggtatttgta
     5341 tcccgccgct ggggatgggg ctggcgacgc tgattggtcg taaaaatttc tccgcagaag
     5401 agcgcgaaac cggcaaagcg gcgctggtga tggggtgcgt tggggttact gaaggggcga
     5461 ttcctttcgc cgctgccgat ccgctgcgtg tcattccttc catcatggtc ggttctgttt
     5521 gtggtgcggt aactgcggcg ctggtcggtg cgcagtgcta tgcaggctgg ggtggtctga
     5581 ttgtgctgcc ggtggttgaa ggcaagctgg gttatatcgc agcagtggct gtcggagcag
     5641 tggtgacggc tgtttgcgtg aacgtgctga aaagtctggc gcgtaaaaat gggtcttcga
     5701 ctgatgagaa ggaagacgac ctggatttgg attttgaaat taattaattg aggaacgagc
     5761 catgacgaaa attattgcag taaccgcgtg tccatctggt gttgcccata cctatatggc
     5821 ggcagaggcg ctggaaagcg ccgcgaaagc gaaaggctgg gaagtgaaag tagaaactca
     5881 ggggtcaatt ggtctggaaa acgaactgac tgcggaagac gtggcgagcg ccgatatggt
     5941 gattctgacc aaagatatcg gcatcaagtt tgaagagcgt tttgctggca agaccatcgt
     6001 gcgcgtcaac atcagcgatg cggtaaaacg tgctgacgcc attatgagca aaattgaagc
     6061 tcacctggcg caaaccgctt aagttcttct cccgctcgca agggcgggtt cgctttccca
     6121 caggagttcc tcatgacgaa tcgtatctct cgcctcaaaa ctgcactgtt ttccaatacc
     6181 cgtgaaatct cgctggagcg ggcgctgctt tataccgcca gccatcggca aaccgaaggc
     6241 gaaccggtga tattgcgccg ggcgaaagca acagcgtata tccttgaaca tgttgaaatt
     6301 tcgattcgtg atgaagaact gattgccggt aaccgcaccg taaaaccgcg cgccgggatt
     6361 atgtcgccgg aaatggaccc ttactggctg ctgaaagagc tggatcaatt cccgacgcgt
     6421 ccgcaggacc gctttgctat cagcgaagaa gataaacgta tctaccgcga agagttgttc
     6481 ccgtactggg aaaaacgttc gatgaaagat ttcatcaacg ggcagatgac agatgaagta
     6541 aaagccgcga ccagcacgca gattttcagc attaaccaga cggataaagg ccaggggcac
     6601 attattattg attacccacg cctgctgaat cacgggctgg gggagctggt ggcgcagatg
     6661 cagcaacatt gtcagcaaca gccggagaat cacttttatc aggccgcgct gttactgctg
     6721 gaagcctcgc agaaacacat tctgcgttac gctgaactgg cggaaacaat ggcggcaaac
     6781 tgcacagatg cccagcgtcg cgaagaactg ctgactattg cggagatctc ccgccataac
     6841 gccgaacata agccgcagac gttctggcag gcgtgccagt tattctggta catgaacatc
     6901 attctgcaat acgaatccaa cgccagttcg ctgtctttag ggcgcttcga ccagtatatg
     6961 ttgccgttct atcaggcttc tttaacccag ggcgaagatc cggcgttcct gaaagaactg
     7021 ctcgaatctt tatgggtgaa atgcaacgac atcgtgctgt tgcgctccac cagcagcgcg
     7081 cgttatttcg caggtttccc gaccggctat accgcactgc tcggcgggtt aaccgagaac
     7141 ggacgtagcg cggtgaacgt gctttcgttc ctttgccttg acgcctatca aagcgtgcaa
     7201 ttaccgcaac cgaacctcgg cgtgcgcact aacgccttga tcgacacgcc gttcctgatg
     7261 aaaaccgccg aaaccattcg cctcggcacc ggtattccgc aaatctttaa cgatgaagtg
     7321 gtggtaccag cgttcctcaa ccgtggcgtt tcgctggaag atgcgcgcga ctattccgta
     7381 gtgggctgtg tggaattatc tattcccggc agaacctacg gcttgcatga catcgcgatg
     7441 tttaatctgc tgaaagtgat ggaaatctgc ctgcatgaaa atgaaggcaa tgctgcgctg
     7501 acttatgaag gtttactgga gcagatccgc gccaagatca gccactacat caccctgatg
     7561 gttgagggca gcaatatttg tgatatcggc catcgcgact gggcacctgt accgctgctc
     7621 tcgtctttta tcagcgattg tctggaaaaa ggtcgcgata ttaccgatgg cggcgcgcgt
     7681 tataacttct ccggcgtaca ggggatcggt atcgccaacc tgagcgattc tctccatgcg
     7741 ttgaaaggga tggtttttga gcaacagcgt ttaagttttg acgaattgct gtcggtatta
     7801 aaagccaact ttgcaacgcc agaaggcgaa aaagtccgtg ctcgcttaat taaccgcttc
     7861 gagaaatacg gtaacgatat cgacgaggtg gataacatca gcgccgaact gttacgccac
     7921 tactgcaaag aagtggaaaa ataccagaac ccgcgcggtg gttacttcac gccgggatcg
     7981 tataccgttt ctgctcacgt cccgttggga tcggtggttg gcgcgacgcc agacggtcgt
     8041 tttgccgggg aacaactggc agacggcggc ttgtcaccta tgctgggcca ggacgcacaa
     8101 gggccaacag cggtactgaa gtcagtcagt aagctcgata atacgctgct gtctaacggt
     8161 acgttgctga acgtgaaatt cactccggcg accctggaag gtgaagcggg attacgcaaa
     8221 ctggccgact tcttacgggc gtttacccag cttaagttgc agcatattca gtttaacgtg
     8281 gtgaacgccg acacgttgcg ggaagcgcaa cagcgcccac aagattatgc cgggctggtg
     8341 gtgcgcgttg ccggatacag cgccttcttt gtcgaactgt cgaaggagat ccaggatgac
     8401 atcatccgcc ggacagcgca tcagctgtaa cgttgtggaa acgcgccgca atgatgtggc
     8461 gcgcattttc aacattcagc gttattcact gaatgacggc gagggcattc gtacggtggt
     8521 cttttttaaa ggctgtccgc atctttgccc atggtgtgct aatccggagt cgatctccgg
     8581 caaaatccag acggtacgca gagaggcgaa atgtctgcac tgtgcgaaat gtttgcgtga
     8641 tgcggatgaa tgcccctccg gggcgtttga acggattggt cgcgatatca gccttgacgc
     8701 gctggaacgg gaagtgatga aagatgacat tttctttcgc acgtccggcg gcggcgtcac
     8761 gctttctggc ggcgaagtgt taatgcaggc ggagtttgct acccgttttt tacagcggct
     8821 gcggttgtgg ggcgtgtcat gcgccattga aactgccgga gacgtgccag ccagcaagct
     8881 gttaccactg gcgaaattgt gcgatgaagt gctgttcgat ttaaaaatta tggacgcgac
     8941 tcaggcgcgg gatgtggtga agatgaacct gccacgcgtg ctggagaatc tgcgtttgct
     9001 ggtgagtgag ggtgtcaacg tgatcccgcg tttaccgttg atccctggtt tcacgctcag
     9061 ccgggagaat atgcagcagg cgctggatgt actgatcccg ctgaaaatca ggcagatcca
     9121 tctgttaccg tttcatcagt acggcgaacc gaaataccgc ctgctgggga aaacatggtc
     9181 gatgaaagag gtgcctgcgc cgtcgtcagc cgatgtgaca acgatgcgcg aaatggcaga
     9241 acgggccgga tttcaggtta ccgtgggagg ttaaaatggc atacctggtg gcagtaaccg
     9301 cctgcgtaag cggcgtggcg catacttata tggcggcgga acggctggaa aagttgtgcc
     9361 agttagagaa gtggggcgtt agcattgaaa ctcagggcgc gctgggaacg gagaatcgtt
     9421 tagcggacga ggatatccgt cgggcggatg ttgctctgtt gattacggat atcgagcttg
     9481 ccggtgccga gcgatttgaa cattgccgct atgtgcaatg cagcatctac gcattcctgc
     9541 gtgagccgca gcgggtaatg agcgcggtgc gcaaagtgct ttctgcgccg cagcaaaccc
     9601 atcttattct ggagtagtcg gtttttctgt cagttggctg tggtactgcc gacgatattc
     9661 cgacggcgag cgttcggtgt ttttacgaaa cagacggcag aagtagttgc tgtcgacaaa
     9721 gccgcaggcg tgcgccactt cttttacctt caggtcatag cctttcaata acgtcttagc
     9781 gtgctccagt cgcgtgtgat tcaggtattc gttaaaacca atggcccccg ttttttgaaa
     9841 caggtgcgag agatagtttg gtgaaatata gaatgcctgt gcgacagatt cgcgggtaag
     9901 cgcggaggca tagcgttcgt cgatataatc gcgaatagct tcaaatagtg cctggctgcg
     9961 tgaggcggtc tggatttggc tgccgagcaa atcgcggcag tggctaagca ggctggcgac
    10021 gataagcctt gccgtttgct gctcctgcgg ctgcatttgc atttcattga gcgtttgtag
    10081 cagaaaagaa cctatgcgtg ggccgcgccg ggcgacgtgt tgcttcgcca gattttgata
    10141 ttgtttgcca tcccattgca cgacgctgaa gccgagttgc tgtttgccaa acagcacgct
    10201 aaaggtagta gcgggggctt gccattgtgg aaaattccag ccaccagcag gaacgtacag
    10261 cacatcgccg ggaactaacg ttgctccagc gccggtatca acaaactcac cttccagcac
    10321 aatttccagc cgtggaaaat cgacctgata cgcgagatcg ggcaccgggc cgttactgct
    10381 ggcaaagtaa atctggcgca gggacagcgg gccgttgatc aggcgggaga gcagatagct
    10441 gacgtcgtga tacatgttat tcccttaggt atgtagacgg gaaaacagta actggcatta
    10501 ttaaaaaagg ccacctgaaa agtgtggcct gaggggagtt cgatgtaaac attgcggcag
    10561 tatcgcatcc ggcaaatagc gccagacgcg atacaaaggt catttactga ttacccacct
    10621 gatcgccata cggcagcgtg tcgtaatcga tcagcgcgtt tttcttgtac gggttaccaa
    10681 tccagcgggt agtttctttg aactgcggat tcaccactga acgggttgga tcgtaaccgt
    10741 cgtaagataa gcctgctaaa tctgaccagg tgtggatcag ttccgccagg ctgtatttac
    10801 gatcaacatc ctgcgagaaa tcacgcggat gagtcgcttg ccatttttcc gacgtccaca
    10861 gcaggaacgg aatggtgtac atgtggcgcg tcgggttgtc ttcgttacgc ccctgagttt
    10921 tgtgcggcgg cgtgtcgtaa acctcttcac cgtggtcaga gaaataaacc aggaaaccgt
    10981 tcgggtttgc tgctttaaag tctttaatca ggctggcaac cacatgatcg ttatacaggt
    11041 tagcgttgtc ataatcgtta tatgactcca gctcttccgc gtttaatcca ggcggaacat
    11101 gatcggtatt gccatcaaac ttgccctgat tttccgggta gcggtatttg tatttgatat
    11161 gcgtacccag cagatgaacg atgatcagtt tcttcggcgc cgggtccttc agcacttcct
    11221 ggaatggttt cagcacgttg gtgtcgtatt cacgcgcact ctgcgtacgt tgctggttca
    11281 tatagaactg tttgtcggtc tgcttcgaaa ataccgtcag catggtattg cgggcggtca
    11341 tcgtctgctg gttggtgatc cagaaggttt tataacctgc ctgtttcatc atgttcatca
    11401 gcgacggttg tgtcaggtac aggtccgggt tcttttcatt agcaaaggtc agcgcctgtt
    11461 gcaggatttc aatggtgtac ggacgagacg taaccacgtt attaaacacg gtcagattcg
    11521 gatcggtttt atgcagcgca tccagctccg gcgtggtttc acgcggataa ccgtacagac
    11581 tcatgcgccc gcgttgggtc gattcgccaa tcaccagcac taaggtacgc ggttcgttac
    11641 ccgattcatc tttgaaatta gccagaggcg gcaaggcatt attttcattc agtaactttg
    11701 ttagcgagtt tagttgctga cgatactgat aatagccggt caggaattgc cacggtgcgg
    11761 caggctccat acgcgaggcc aggttatcca acgttttctc aaacggcttg tttttgataa
    11821 acgtattcat ggcgatcgga tgcagaatca agccataaag cagggcaaaa gagacaacat
    11881 aacgccacgg ctttggaata tagaccgggt gcaggcgtgt ccacagcaga actgccaccg
    11941 ccgtataggc cagcgcgata agcacaattt tcaggctgaa atactggctt aaatactcgc
    12001 tggcttcgtt ggtgttggtt tcgaacatca caaacagaac gctctgcgag aactcctgcc
    12061 cgtagatgac gtagtagcat agcgccgcca gagaggccgc ccatagcacc acgccgatta
    12121 ctgcggcaat aattttaatc cgcttcggaa agaggaatac cgggatcaac cacagcgagc
    12181 tgaataacag cgagtcgcga atgccgttgg tgccactata accactgatg taaataatgg
    12241 cctgtagcag agtagagaaa aaccaaaagt agagcagtgc ccaacccagg gctttccagc
    12301 taaaaagggg tttagcctgg acttctgtgg aatgcatagt aagaacctgt cttgaaaaaa
    12361 atatcgccga atgtaacgac aattccttaa ggatatctga aggtatattc agaatttgaa
    12421 taaaatgcag acagaaatat attgaaaacg agggtgttag aacagaagta tttcagaaaa
    12481 ccctcgcgca aaagcacgag ggtttgcaga agaggaagat tagccggtat tacgcatacc
    12541 tgccgcaatc ccggcaatag tgaccattaa cgcttgttcg acgcgaggat ccggttcctg
    12601 gccttctttt tctgcctggc gggagcggtg cagtaactcg gcctgcaata cgttcagcgg
    12661 gtcggtgtaa atattccgta gctgaataga ctctgcaatc cacggcagat cggccattag
    12721 atgggaatcg ttggcaatcg ccagcaccac tttgatgtct tcttcttgca ggttgcgtaa
    12781 ctctttacct aacggccaca gtgctttgtc taccaggcgt tggtcatagt attccgccag
    12841 ccacaggtct gctttggcga agaccatctc cagcatgccg agacgcgtcg agaagaatgg
    12901 ccaatcgcgg cacatggctt ccagctcgct ctgtttgcca tcttcgacca ctttttgcag
    12961 cgccgtacct gcacccagcc aggcggggag catcaggcgg ttttgcgtcc aggcaaaaat
    13021 ccacggtatg gcgcgcagcg actcgacgcc gccggttggg cgacgtttcg ccggacgtga
    13081 acccaacggc agtttgccca attcttgttc cggcgtagcg gagcggaagt aaggcacaaa
    13141 atctttgttt tcacgtacgt agccacggta gagatcgcag gagatgactg acagttcatc
    13201 cataatgcga cgccagctct ctttcggctc aggtggtggc agcaggttgg cttccagaat
    13261 agccccggtg tacagcgaca ggctgctgac ggtgatttct ggcagaccat atttaaagcg
    13321 gatcatctcg ccctgttcag tcacgcgcag gccacctttc aggcttcctg gcggttgtga
    13381 cagcagcgcc gcatgagcag gtgcgccgcc gcgaccaatg gaaccgccgc gaccgtggaa
    13441 caacgtcagc tcaatacccg ctttttcgca ggttttgatt aatgcatcct gtgcctgata
    13501 ttgcgcccag gaagctgcca tcactcccgc atcttttgct gagtcggaat agccaatcat
    13561 caccatctgt ttgccctgaa tcaggccacg ataccagtca atattgagca gctgggtcat
    13621 gacatcgttg gcgttgttca gatcatcgag ggtttcaaac agcggagcaa ccggcatcgc
    13681 aaacccgata cccgcttctt tcagcagcag gtggacagcc agtacgtcgg acggcgtttt
    13741 cgccatcgag atcacgtagg cggcaatgga gccttgcggt gcttcggcaa tcacctggca
    13801 ggtatcgagc acttcgcgcg tttcggcgct tggttgccag ttgcgcggca gaagcggacg
    13861 tttggagttc agttcgcgga tcaggaacgc ctgtttgtcg gcctctgacc agctttcgta
    13921 gtcgccgata ccgaggtagc gggtcagctc gcccagcgct tcggtatgac gcgtgctctc
    13981 ctgacggata tcaatacgga ccagcggtac gccgaaacat ttcacgcggc gcagggtgtc
    14041 gagcagatcg ccgttggcga taatacccat gccacacgcc tgaagtgact ggtagcaagc
    14101 gtagagcggt tcccacagtt cttcgttttg tgtcagcagg ccttctggtt ttggcagttc
    14161 ttcgcctttc aggcgcgctt ccagccatgc ctgtgtcgcc atcaggcgag aacgcaggtt
    14221 tttcatcaga tagcgatacg gttctgcggc accttcttcg ccaaccagcg ccagcagttc
    14281 aggggtcgct tcaaccatcg acagttcaga aaccagcacc tgaatatctt tcaggaacaa
    14341 atcggtggct ttccagcggc tgagtagcag gacgtggcgg gtgatatcgg cagtgacgtt
    14401 cgggttgccg tcgcggtcgc cgcccatcca cgaagtaaaa cggaccggaa caaattcgac
    14461 gggcagtttg tagccgaggt tctcttccag ttgttcgttc agttcgcgca ggtaatttgg
    14521 tacgccttgc cacaggctgt tttccactac ggcaaagccc catttggctt catctaccgg
    14581 gcttggacgc agcttacgga tttcatcggt atgccatgac tgggcgatta actggcgcag
    14641 gcggcgcatc agctggttgt gttcgtagtc agcgatatct ttgttatcga gctgttttaa
    14701 acaggcgttc acttccacca ttttgtggat cagtgtacga cgggtaattt cggttgggtg
    14761 agccgtgagg accagttcca gcgacagcga ttccactgct tttttgatgg tgtcttcgct
    14821 cagttccggc tggtttttca gtttacgcag ggtgcgggcg atcacttccg ggttgctggc
    14881 agcttcgcct ttcggcgaaa tgctgtggta ttgctcggcg gtgttggcca ggttcaggaa
    14941 ctgactaaac gcacgcgcaa cgggcagcag ctcgtcgttc gacaaatttt gtaaggtggt
    15001 gagcaactcc tggcggttag catcattgcc agcgcgtgaa gatttcgaca acttacggat
    15061 agtttctacg cgttcaagaa tgtgttctcc caacgcatcc ttgatggttt ctcccagcac
    15121 tttgccgagc atactgacat tactacgcaa tgcggaatat tgttcgttca tattacccca
    15181 gacaccccat cttatcgttt gatagccctg tatccttcac gtcgcattgg cgcgaatatg
    15241 ctcgggcttt gcttttcgtc gtcttttata aagccacgta aaagcggtga cgtcaaatgc
    15301 tgcgaaatcg cttcagcaaa cgaataaata gcaggaattt acgccattaa attcacgacg
    15361 ctttaaataa gcgtaactta tggaaatgtt aaaaaatcgc catacataac accaaaggtg
    15421 taggtcggat aagatgcgca agcatcgcat ccgacattat tgcggcacag gagtttggca
    15481 acggtgccgg atgcggcgcg agcgccttat ccggcctatg ttttaatgcc agcaaaaatg
    15541 gtgaattacc tgggttatca gttcgcgggt gggcttgata aaccgtgttt ccagatattc
    15601 atcaggttga tgagcctgat taattgagcc aggccccaac accagcgtcg ggcataacgt
    15661 ttgaataaac ggcgcttcgg tacagtagtt caccacttcg gtctttgctc cgagcaattt
    15721 ctcaactact tcaaccagtt gatgattcgg tgggcattca tagccaggga tcggcggatg
    15781 cagctcgtcg accgtcagac gacccggcca gcgttcgctc accggagcca atgcatcgtt
    15841 gagcaaacca ttaagttcat tgagtgtcat gccaggcagc ggacgaatat ccatatgcaa
    15901 ctcacagcaa gcgcaaatac ggttagaagc gtcgccaccg tgaatatgcc cgaggttgag
    15961 cgtagggtat ggcacggtaa acgcttcgta gtgataacgt tctttcaggt tatcgcgcaa
    16021 ttgcaaaata tgcccgatgg cgtcgtgcat tagttcgata gcgttaactc cgcgtgctgg
    16081 atcgctggag tgccccgact ggccctgaat acggatggcg ttagagatat gacctttatg
    16141 tgcgcgtacc ggttgtagtg acgtcggttc gccaatgatg gcgcaatccg ggcgcagggc
    16201 ggtagtttcg gcaaaataac gcgctccggc catactggtt tcttcatcag cagtcgccag
    16261 aatgtagagc ggttttttca gtttcgtgac gtcgacatcg cgtagcgcat caaggataaa
    16321 cgcaaaaaag cctttcatgt cggcggtgcc taagccgtaa agcttgccgt catgctccgt
    16381 cagtgtaaac ggatcgcgcg tccagcgacc gtcatcaaat ggcaccgtat cggtatgccc
    16441 cgccagcaac aagccgccag ccccctgtcc gatacttgcc agcatattga atttgttgcg
    16501 agttcctggc acaggctgca cttccacatt gaagcctaaa tctttaaacc agtccgccag
    16561 cagagtgatt aaatctgcat tgctttgatc gagtgcctct tccgtggcgc ttattgaagg
    16621 tgtggcaatc agagcacggt aaatctcgat aaatggcggt aatttgtttt tcattgttga
    16681 cacacctctg gtcatgatag tatcaatatt catgcagtat ttatgaataa aaatacacta
    16741 acgttgagcg taataaaacc caccagccgt aaggtgaatg ttttacgttt aacctggcaa
    16801 ccagacataa gaaggtgaat agccccgatg ttgaatacgc tgattgtggg tgccagcggc
    16861 tacgctggcg cagagctagt gacctatgta aatcgccatc cgcatatgaa cataaccgct
    16921 ttgactgttt cagcgcaaag caatgatgcg ggaaagttaa tctccgattt gcatccgcag
    16981 ctaaaaggca tcgttgatct gccgttgcag ccgatgtcgg atatcagcga gtttagccca
    17041 ggggtggacg tagtgtttct cgccaccgcc catgaagtta gccacgattt agcgccgcag
    17101 tttcttgaag cgggctgcgt ggtgttcgac ctttccggcg cgtttcgtgt taacgacgcc
    17161 accttctatg aaaaatatta cggctttacc catcaatacc cggaactgtt ggaacaggca
    17221 gcctacggtc tggcggagtg gtgcggtaat aaattaaaag aagcgaattt gattgcggtg
    17281 ccgggctgtt atccgacggc ggcacagctg gcgctgaaac cgttgattga tgccgatctt
    17341 cttgacctca atcagtggcc ggtgatcaac gccaccagcg gcgtgagcgg tgcaggacgt
    17401 aaggcagcga tttcaaatag tttttgtgaa gttagcctgc aaccgtatgg cgtctttacc
    17461 catcgccatc aaccagagat cgccacacac ctcggtgctg acgttatctt caccccgcat
    17521 ctgggcaatt tcccgcgcgg cattcttgaa accattacct gccgcctgaa accgggtgtg
    17581 acccaggcgc aagtggcgca agcgttacag caggcgtatg cccataaacc gctggtgcgg
    17641 ctgtatgaca aaggcgttcc ggcgctgaaa aatgtcgttg ggctgccatg ttgcgatatc
    17701 gggtttgccg ttcagggcga gcatctgatt attgtggcga ccgaagacaa cttactgaaa
    17761 ggcgcggcgg cacaagcggt acagtgcgcc aatattcgtt tcggctatgc ggaaacgcag
    17821 tctcttattt aagggtgcaa tgatgaatcc attaattatc aaactgggcg gcgtactgct
    17881 ggatagtgaa gaggcgctgg aacgtctgtt tagcgcactg gtgaattatc gtgagtcaca
    17941 tcagcgtccg ctggtgattg tgcacggcgg cggttgcgtg gtggatgagc tgatgaaagg
    18001 gctgaatctg ccggtgaaaa agaaaaatgg cctgcgggtg acgcctgcgg atcagataga
    18061 cattatcacc ggagcactgg caggaacggc aaataaaacc ctgctggcat gggcgaagaa
    18121 acatcagatt gcggccgtag gtttgtttct cggtgacggc gacagcgtca aagtgaccca
    18181 gcttgatgaa gagttaggtc atgttggact ggcgcagcca ggttcgccta agcttatcaa
    18241 caccttgctg gagaacggct atctgccggt ggtcagctcc attggcgtaa cagacgaagg
    18301 gcaactgatg aacgtcaatg ccgaccaggc ggcgacggcg ctggcggcaa cgctgggcgc
    18361 ggatctgatt ttgctctctg atgtcagcgg cattctcgac ggcaaagggc aacgcattgc
    18421 cgaaatgacc gccgcgaaag cagaacaact gattgagcag ggcattatta ctgacggcat
    18481 gatagtgaaa gtgaacgcgg cgctggatgc tgcgcgcacg ttgggccgtc cggtagatat
    18541 cgcctcctgg cgtcatgcgg aacagcttcc ggcactgttt aacggtatgc cgatgggtac
    18601 gcggatttta gcttaagttt tgttggccgg aggcgcagct ttccggcatt gaatttcaaa
    18661 ataaggaaac agagttatgg cactttgggg cgggcgtttt acccaggcag cagatcaacg
    18721 gttcaaacaa ttcaacgact cactgcgctt tgattaccgt ctggcggagc aggatattgt
    18781 tggctctgtg gcctggtcca aagccctggt aacggtcggc gtgttaaccg cagaagagca
    18841 ggcgcaactg gaagaggcgc tgaacgtgtt gctggaagat gttcgcgcca ggccacaaca
    18901 aatccttgaa agcgacgccg aagatatcca tagctgggtg gaaggcaaac tgatcgacaa
    18961 agtgggccag ttaggcaaaa agctgcatac cgggcgtagc cgtaatgatc aggtagcgac
    19021 agacctgaaa ctgtggtgca aagataccgt tggcgagttg ctgacggcta accggcagct
    19081 gcaatcggcg ctggtggaaa ccgcacaaaa caatcaggac gcggtgatgc caggttacac
    19141 tcacctgcaa cgcgcccagc cggtgacgtt cgcgcactgg tgcctggcct atgttgagat
    19201 gctggcgcgt gatgaaagcc gtttgcagga tgcgcttaag cgtctggatg tcagcccatt
    19261 aggctgtggc gcgctggcgg gaacggccta tgaaatcgac cgtgaacagt tagcaggctg
    19321 gctgggcttt gcttcggcga cccgtaacag tctcgacagc gtttctgacc gtgaccatgt
    19381 gttggaactg ctttctgctg ccgctatcgg catggtgcat ctgtcgcgtt ttgctgaaga
    19441 tctgattttc tttaacaccg gcgaagcggg gtttgtggag ctttctgacc gcgtgacttc
    19501 cggttcatca ttaatgccgc agaagaaaaa cccggatgcg ctggagctga ttcgcggtaa
    19561 atgcggtcgg gtgcaggggg cgttaaccgg catgatgatg acgctgaaag gtttgccgct
    19621 ggcttacaac aaagatatgc aggaagacaa agaaggtctg ttcgacgcgc tcgatacctg
    19681 gctggactgc ctgcatatgg cggcgctggt gctggacggc attcaggtga aacgtccacg
    19741 ttgccaggaa gcggctcagc agggttacgc caacgccacc gaactggcgg attatctggt
    19801 ggcgaaaggc gtaccgttcc gcgaggcgca ccatattgtt ggtgaagcgg tggtggaagc
    19861 cattcgtcag ggcaaaccgc tggaagatct gccgctcgac gagttgcaga aattcagccc
    19921 ggtgattgac gaagatgtct atccgattct gtcgctgcaa tcgtgcctcg acaagcgtgc
    19981 ggcaaaaggc ggcgtctcac cgcagcaggt ggcgcaggca attgcttttg cgcaggcgcg
    20041 gttggggtaa gaacatttat atgtataaat ttgagcctgg cttatcgccg ggctttttta
    20101 tggcaaaaaa aagcggatcc tggagatccg caaaagttca cgttggcttt agttattcga
    20161 gttgagaaac tctcgaaacg ggcagtgact tcaagggtta aaagaggtgc cgctccgttt
    20221 ctgtgagcaa ttatcagtca gaatgcttga tagggataat cgttcattgc tattctacct
    20281 atcgccatga actatcgtgg caatggagga tggataatga atattcgtga tcttgagtac
    20341 ctggtggcat tggccgaaca ccgccatttt cggcgtgcgg cagattcctg ccacgttagc
    20401 cagccgacgc ttagcgggca aattcgtaag ctggaagatg agttgggcgt gatgttgctg
    20461 gagcggacca gccgtaaagt gttgttcacc caggcgggaa tgctgctggt agatcaggcg
    20521 cgtaccgtac tgcgtgaggt gaaagtcctt aaagagatgg caagccagca gggcgagacg
    20581 atgtccgggc cgctgcacat tggtttgatt cccacagttg gaccgtacct gctaccgcat
    20641 attatcccta tgctgcacca gacctttcca aagctggaaa tgtatctgca tgaagcacag
    20701 acccaccagt tactggcgca actggacagc ggcaaactcg attgcgtgat cctcgcgctg
    20761 gtgaaagaga gcgaagcatt cattgaagtg ccgttgtttg atgagccaat gttgctggct
    20821 atctatgaag atcacccgtg ggcgaaccgc gaatgcgtac cgatggccga tctggcaggg
    20881 gaaaaactgc tgatgctgga agatggtcac tgtttgcgcg atcaggcaat gggtttctgt
    20941 tttgaagccg gggcggatga agatacacac ttccgcgcga ccagcctgga gacgctgcgc
    21001 aacatggtgg cggcaggtag cgggatcact ttactgcctg cgctggctgt gccgccggag
    21061 cgcaaacgcg atggggttgt ttatctgccg tgcattaagc cggaaccacg ccgcactatt
    21121 ggcctggttt atcgtcctgg ctcaccgctg cgcagccgct atgagcagct ggcagaggcc
    21181 atccgcgcac gaatggatgg ccatttcgat aaagttttaa aacaggcggt ttaaaccgtt
    21241 taacgcagct acccgatagg cttccgccat cgtcgggtag ttaaaggtgg tgttgacgaa
    21301 gtactcaata gtgttgccgc cacctttctg ttccataatc gcctgaccga tatgaataat
    21361 ttcggcagcg cgctcgccaa agcagtgaat acccagaatc tcttttgttt cccgatggaa
    21421 caaaattttc agcgtgccca cgttcatgcc gacgatttgt gcgcgtgcca gatgtttaaa
    21481 ctgggcgcgg cccacttcat atggcacttt cattgcggtc agctgctgtt cggttttgcc
    21541 cacagagctg atttccggga tggtgtaaat gccggtaggg atatcttcaa tcagatgtgc
    21601 ggtggcttcg ccttttacca gcgcctgcgc ggcaatgcgc ccctggtcat aggccgctga
    21661 tgccaggctc ggataaccaa tcacgtcgcc caccgcgtaa acgtgcggct gtgcggtctg
    21721 atacatgctg ttgaccttca gctgtccgcg gctgtcagtt tccagcccaa tgttctgtaa
    21781 cgccagcgaa tcggtattac cggtgcgacc gttggcatag agcaggcagt cagctttcag
    21841 ttttttaccc gacttcaaat gcatgatcac accatcgtca cagccttcga tcttctcgta
    21901 ctcttcgttg tgacgaatca ctacgccact gttccagaag tgataggaga gagaatctga
    21961 catctcttga tcgagaaatg ccagcaggcg atcgcgggtg ttgatcagat ccacttttac
    22021 atccataccg cggaagatcg acgcatattc acagccgatc actccagcac catagataag
    22081 tacatggcgc ggttcgtggt gcatgctaag aattgagtcg ctgtcgtaaa tgcgtggatg
    22141 ggtgaaatca acatctgttg gatgatatgg acgagagccg caggcaataa caaatttttc
    22201 agcggttagt gtttcaacgc tgccgtccgg gcaatccagc gccaacgtat gctcgtcaac
    22261 aaagcgagcg tttccctgca atatttcaca gtgattacgt tcgtaaaatc cctgacgcat
    22321 gcgcgtttgt tgattaatca cgttatcggc atggttaagg atatcggcaa aagaagagcg
    22381 gagcagtcgg gaatggtcgc tgtaaagtgg gttttgattg aattctataa tgcggctgac
    22441 ggcgtgacgg agagctttcg acgggatggt gccccagtgg gtgcaaccgc cgccaacatt
    22501 ttgataacgc tcgataactg cgacgcgcgc accttgctta accaggccca ttgcagcgcc
    22561 ttcgccgccg gggccggaac ctattactat ggcatcgtaa tcgtaggaat gtggcatggt
    22621 agggcttacc tgttcttata cataaaagca acagaatggt aacattttat cgcgggtaag
    22681 ccaattgatc cccgtcattt atctggctat atcctgagcg gcctttgctt tgtctgtttc
    22741 ttacttttgc cctgacgttt tattggattt ttatcgacga tactctccgt ttaagcggca
    22801 ggtttccgct gtacgtaaaa gaaccggcca aagaattgca gtaaatatgt tttattgcgt
    22861 taccgttcat tcacaatact ggagcaatcc agtatgttca ttctctggta tagtgccagc
    22921 agtacttttg gcaaggattc agacatcgtg atgggcgtaa gagcgcaaca aaaagaaaaa
    22981 acccgccgtt cgctggtgga agccgcattt agccaattaa gtgctgaacg cagcttcacc
    23041 agcctgagtt tgcgtgaagt ggcgcgtgaa gcgggcattg ctcccacctc tttttatcgg
    23101 catttccgcg acgtagacga actgggtctg accatggttg atgagagcgg tttaatgcta
    23161 cgccaactca tgcgccaggc gcgtcagcgt atcgccaaag gcgggagtgt gatccgcacc
    23221 tcggtctcca catttatgga gttcatcggt aataatccta acgccttccg gttattattg
    23281 cgggaacgct ccggcacctc cgctgcgttt cgtgccgccg ttgcgcgtga aattcagcac
    23341 ttcattgcgg aacttgcgga ctatctggaa ctcgaaaacc atatgccgcg tgcgtttact
    23401 gaagcgcaag ccgaagcaat ggtgacaatt gtcttcagtg cgggtgccga ggcgttggac
    23461 gtcggcgtcg aacaacgtcg gcaattagaa gagcgactgg tactgcaact gcgaatgatt
    23521 tcgaaagggg cttattactg gtatcgccgt gaacaagaga aaaccgcaat tattccggga
    23581 aatgtgaagg acgagtaatg aaacaagcaa atcaagatag aggtacgctg ctgctggcgt
    23641 tagttgctgg cttatcgatt aatggtactt tcgcagcgct gtttagctcc attgtgccat
    23701 tttctgtatt cccgattatt tccctggtgc tgacggttta ctgcctgcat caacgttatc
    23761 ttaatcgcac tatgccggta ggcttgccgg gtctggcagc tgcctgtttt attctcggcg
    23821 tactgctgta cagcacggta gttcgtgcgg aatatccgga tatcggctct aacttcttcc
    23881 cggcagtact ctccgtcatt atggtgttct ggattggcgc gaagatgcgt aaccgtaagc
    23941 aggaagttgc tgaataatcg gtattatgcc ggatgctggc gcatccagca tgggttttac
    24001 ttcgcggtca gtaatacgcc gcactccata tggtgcgtgt aggggaactg atcaaacaga
    24061 gccagacgtt cgaccttgtg cgtctggctt aatgtttcca gattcttgca taacgtttcc
    24121 gggttacagg agatgtacaa aatacacgga tacgcctgca ccattttctc ggtttcactg
    24181 tccagaccgc tgcgcggtgg gtcgacaaaa atggtttcgc actgataact ctttaagtcg
    24241 atcccttgca ggcggttaaa ctcgcgcaca ccattcatcg cctgagtaaa ttcttctgcc
    24301 gccatacgaa taatttgtac gttatcaata tggttagctg cgatgttgta ttgcgcagca
    24361 gcaaccgacg gcttagcgat ttcggtggct aatacccgat caaaattgcg cgccagcgct
    24421 aatgaaaagt taccgttgcc gcagtacagc tccagtaaat cgccttttga gcctttggtt
    24481 acgtccagcg cccattccag catctgaata ttcatcgccg cgttcggctg ggtaaagctg
    24541 ttttctacct gacggtagat catctctttc cctgcgaccg gcagacgctc gtcgatgtaa
    24601 tcttgatcca gctcgatttt ggttttcgtt gcccgaccaa tcagatgcac attcagattc
    24661 tgcgcgcgca gtgcatcgcg cagggcctcc gcttcctgac gccactcatc atccagcttc
    24721 ttatggtata gcagggaaac caccgcctga ttactcagcg tggtgaggta atcaatctgg
    24781 aacaacttgt ggcgcagaac gggattatta cgcacacccg caatcatcgc cgtcatcaac
    24841 tggttgataa gttcactggc ggcggggaag ctatccacgc ggatgcggct tttggtttgt
    24901 tgatcgaaaa tgatgtgata caggtcatcg ccatcgtgcc agatgcggaa ctccgcgcgc
    24961 atccggtaat gactgaccgg cgagcgaaac acttccggaa ccaggtcaga aaacggtgcc
    25021 atcatacttt gcaaacgtac cactttttcg gctaactgcg cttcatactg ttctgttgga
    25081 aggtgttcgg gggtcatgat gtatcctgaa aaattaaagt acgcggcgat tgtagggatt
    25141 gctcatcaga tgtccagatc ttgatgaatt cctatttgtg agctacgtct ggacagtaac
    25201 ttgttacaac ctgtagcatc cacttgccgg tcctgtgagt taatagggaa tccagtgcga
    25261 atctggagct gacgcgcagc ggtaaggaaa ggtgcgatga ttgcgttatg cggacactgc
    25321 catccggtgg gaagtcatca tctcttagta tcttagatac ccctccaagc ccgaagacct
    25381 gccggccaac gtcgcatctg gttctcatca tcgcgtaata ttgatgaaac ctgcggcatc
    25441 cttcttctat tgtggatgct ttacaatgat taaaaaagct tcgctgctga cggcgtgttc
    25501 tgtcacagcc ttttccgctt gggcacagga taccagcccg gatactctcg tcgttactgc
    25561 taaccgtttt gaacagccgc gcagcactgt gcttgcacca accaccgttg tgacccgtca
    25621 ggatatcgac cgctggcagt cgacctcggt caatgatgtg ctgcgccgtc ttccgggcgt
    25681 cgatatcacc caaaacggcg gttcaggtca gctctcatct atttttattc gcggtacaaa
    25741 tgccagtcat gtgttggtgt taattgatgg cgtacgcctg aatctggcgg gggtgagtgg
    25801 ttctgccgac cttagccagt tccctattgc gcttgtccag cgtgttgaat atatccgtgg
    25861 gccacgctcc gccgtttatg gttccgatgc aataggcggg gtggtgaata tcatcacgac
    25921 gcgcgatgaa cccggaacgg aaatttcagc agggtgggga agcaatagtt atcagaacta
    25981 tgatgtctct acgcagcaac aactggggga taagacacgg gtaacgctgt tgggcgatta
    26041 tgcccatact catggttatg atgttgttgc ctatggtaat accggaacgc aagcgcagac
    26101 agataacgat ggttttttaa gtaaaacgct ttatggcgcg ctggagcata actttactga
    26161 tgcctggagc ggctttgtgc gcggctatgg ctatgataac cgtaccaatt atgacgcgta
    26221 ttattctccc ggttcaccgt tgctcgatac ccgtaaactc tatagccaaa gttgggacgc
    26281 cgggctgcgc tataacggcg aactgattaa atcacaactc attaccagct atagccatag
    26341 caaagattac aactacgatc cccattatgg tcgttatgat tcgtcggcga cgctcgatga
    26401 gatgaagcaa tacaccgtcc agtgggcaaa caatgtcatc gttggtcacg gtagtattgg
    26461 tgcgggtgtc gactggcaga aacagactac gacgccgggt acaggttatg ttgagaatgg
    26521 atatgatcaa cgtaataccg gcatctatct gaccgggctg caacaagtcg gcgattttac
    26581 ctttgaaggc gcagcacgca gtgacgataa ctcacagttt ggtcgtcatg gaacctggca
    26641 aaccagcgcc ggttgggaat tcatcgaagg ttatcgcttc attgcttcct acgggacatc
    26701 ttataaggca ccaaatctgg ggcaactgta tggcttctac ggaaatccga atctggaccc
    26761 ggagaaaagc aaacagtggg aaggcgcgtt tgaaggctta accgctgggg tgaactggcg
    26821 tatttccgga tatcgtaacg atgtcagtga cttgatcgat tatgatgatc acaccctgaa
    26881 atattacaac gaagggaaag cgcggattaa gggcgtcgag gcgaccgcca attttgatac
    26941 cggaccactg acgcatactg tgagttatga ttatgtcgat gcgcgcaatg cgattaccga
    27001 cacgccgttg ttacgccgtg ctaaacagca ggtgaaatac cagctcgact ggcagttgta
    27061 tgacttcgac tggggtatta cttatcagta tttaggcact cgctatgata aggattactc
    27121 atcttatcct tatcaaaccg ttaaaatggg cggtgtgagc ttgtgggatc ttgcggttgc
    27181 gtatccggtc acctctcatc tgacagttcg tggtaaaata gccaacctgt tcgacaaaga
    27241 ttatgagaca gtctatggct accaaactgc aggacgggaa tacaccttgt ctggcagcta
    27301 caccttctga accacgtccc accgtgctgg tgtttgactc cggcgtcggt gggttgtcgg
    27361 tctatgacga gatccggcat ctcttaccgg atctccatta catttatgct ttcgataacg
    27421 tcgcttttcc gtatggcgaa aaaagcgaag agtttattgt tgagcgagtg gtggcaattg
    27481 tcaccgcagt gcaagaacgt tatccccttg cgctggctgt ggtcgcttgc aacaccgcca
    27541 gtaccgtttc acttcctgca ttacgcgaaa agttcgactt cccggttgtt ggtgtcgtgc
    27601 cggcgattaa acctgctgca cgtctgacgg caaatggcat tgtcggatta ctggcaaccc
    27661 gcggaactgt taaacgttct tatactcatg agctgatcgc gcgtttcgct aatgaatgcc
    27721 agatagaaat gctgggctcg gcagagatgg ttgagttggc tgaagcgaag cttcatggcg
    27781 aagatgtttc tctggatgca ctaaaacgta tcctacgccc gtggttaaga atgaaagagc
    27841 cgccagatac cgttgtattg ggttgcaccc atttccctct actacaagaa gaactcttac
    27901 aggtgctgcc agagggaacc cggctggtgg attctggcgc agcgattgct cgcagaacgg
    27961 cctggctgtt agaacatgaa gctccggatg caaaatctgc cgatgcgaat attgcctttt
    28021 gtatggcaat gacgccagaa gctgaacaat tattgcccgt tttacagcgt tacggcttcg
    28081 aaacgctcga aaaactggca gttttaggct gatttggttg aatgttgcgc ggtcagaaaa
    28141 ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc accactgaca
    28201 cggaacaacg gcaaacacgt cgccgggtca gcggggttct cctgagaatc tcaacagaga
    28261 aaagcaaaaa atgcttgact ctgtagcggg aaagcgtatt atgcacaccc cgcg
//
LOCUS       DAZGLJ010000038        28265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000038 DAZGLJ010000000
VERSION     DAZGLJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.038"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..702
                     /gene="bcsQ"
                     /locus_tag="ACHK49_001497"
     CDS             <1..702
                     /gene="bcsQ"
                     /locus_tag="ACHK49_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO9262167.1"
                     /translation="ITAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWARA
                     MLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQLKASGRYQ
                     WILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINNFRIGS
                     QVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLANWC
                     LLNYSGLKTPVGSAS"
     gene            699..3317
                     /gene="bcsA"
                     /locus_tag="ACHK49_001498"
     CDS             699..3317
                     /gene="bcsA"
                     /locus_tag="ACHK49_001498"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO9262168.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            3328..5667
                     /gene="bcsB"
                     /locus_tag="ACHK49_001499"
     CDS             3328..5667
                     /gene="bcsB"
                     /locus_tag="ACHK49_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO9262169.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            5674..6780
                     /gene="bcsZ"
                     /locus_tag="ACHK49_001500"
     CDS             5674..6780
                     /gene="bcsZ"
                     /locus_tag="ACHK49_001500"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO9262170.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDIWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            6762..10235
                     /gene="bcsC"
                     /locus_tag="ACHK49_001501"
     CDS             6762..10235
                     /gene="bcsC"
                     /locus_tag="ACHK49_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO9262171.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTLEPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            10317..12305
                     /gene="hmsP"
                     /locus_tag="ACHK49_001502"
     CDS             10317..12305
                     /gene="hmsP"
                     /locus_tag="ACHK49_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO9262172.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSMLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12488..13774
                     /gene="dctA"
                     /locus_tag="ACHK49_001503"
     CDS             12488..13774
                     /gene="dctA"
                     /locus_tag="ACHK49_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO9262173.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13994..15490
                     /gene="yhjJ"
                     /locus_tag="ACHK49_001504"
     CDS             13994..15490
                     /gene="yhjJ"
                     /locus_tag="ACHK49_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO9262174.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(15586..16515)
                     /gene="kdgK"
                     /locus_tag="ACHK49_001505"
     CDS             complement(15586..16515)
                     /gene="kdgK"
                     /locus_tag="ACHK49_001505"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO9262175.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16747..17514
                     /gene="pdeH"
                     /locus_tag="ACHK49_001506"
     CDS             16747..17514
                     /gene="pdeH"
                     /locus_tag="ACHK49_001506"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO9262176.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            17584..19644
                     /gene="yhjG"
                     /locus_tag="ACHK49_001507"
     CDS             17584..19644
                     /gene="yhjG"
                     /locus_tag="ACHK49_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO9262177.1"
                     /translation="MSKAGKITAAISVAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19878..21200)
                     /gene="yhjE"
                     /locus_tag="ACHK49_001508"
     CDS             complement(19878..21200)
                     /gene="yhjE"
                     /locus_tag="ACHK49_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9262178.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21611..22624)
                     /gene="yhjD"
                     /locus_tag="ACHK49_001509"
     CDS             complement(21611..22624)
                     /gene="yhjD"
                     /locus_tag="ACHK49_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO9262179.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22673..23572)
                     /gene="rcdB"
                     /locus_tag="ACHK49_001510"
     CDS             complement(22673..23572)
                     /gene="rcdB"
                     /locus_tag="ACHK49_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9262180.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            24092..24694
                     /gene="yhjB"
                     /locus_tag="ACHK49_001511"
     CDS             24092..24694
                     /gene="yhjB"
                     /locus_tag="ACHK49_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO9262181.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(24745..26394)
                     /gene="treF"
                     /locus_tag="ACHK49_001512"
     CDS             complement(24745..26394)
                     /gene="treF"
                     /locus_tag="ACHK49_001512"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO9262182.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            26799..28196
                     /gene="ccp"
                     /locus_tag="ACHK49_001513"
     CDS             26799..28196
                     /gene="ccp"
                     /locus_tag="ACHK49_001513"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO9262183.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT         6637 a         7163 c         7843 g         6622 t
ORIGIN      
        1 atcaccgccg cattagcctg gtcattacaa atgttgggag aaaatgttct ggtggtcgat
       61 gcctgcccgg acaacttgtt gcgcctgtca tttaatgttg attttaccca ccgtcagggc
      121 tgggccagag cgatgctgga tggccaggac tggcgtgacg ctgggttgcg ctacacctcg
      181 cagctcgatt tgctgccttt tggtcagtta tccattgaag aacaagaaaa tccacagcac
      241 tggcaaaccc ggctgagcga tatttgctcc ggcttacagc aactaaaagc cagcgggcgt
      301 taccagtgga ttttaatcga cttaccgcgt gatgcctcgc agataaccca ccagctgctg
      361 agtttgtgcg atcactcgct ggctatcgtc aatgtggatg ccaactgcca tatccgcctg
      421 catcagcaag cgctgccgga tggcgcacat attttgatta ataacttccg tattggcagt
      481 caggttcagg acgatattta ccagctttgg ttgcaaagcc agcgccgatt gctgccgatg
      541 ctcattcatc gtgatgaagc gatggctgaa tgcctggcgg ctaagcaacc agtcggtgaa
      601 taccgcagtg atgcgctggc ggcagaagag atactgacgc tggcgaactg gtgcctgttg
      661 aactattccg ggctgaaaac gccagtcggg agtgcatcat gagtatcctg acccggtggt
      721 tgcttatccc gccggtcaac gcgcggctta tcgggcgtta tcgcgattat cgtcgccacg
      781 gtgcgtcggc tttcagcgcg acgctcggct gtttctggat gatcctggcc tggattttta
      841 ttccgctgga gcacccgcgc tggcagcgta ttcgcgcaga acataaaaac ctgtatccgc
      901 atatcaacgc ctcgcgtccg cgtccgctgg acccggtccg ttatctcatt caaacatgct
      961 ggttattgat cggtgcatcg cgcaaagaaa cgccgaaacc gcgcaggcgg gcattttcag
     1021 gtctgcaaaa tattcgtgga cgttaccatc aatggatgaa cgagctgcct gagcgcgtta
     1081 gccataaaac acagcatctg gatgagaaaa aagagctcgg tcatttgagt gccggggcgc
     1141 ggcggttgat cctcggtatc atcgtcacct tctcgctgat tctggcgtta atctgcgtta
     1201 ctcagccgtt taacccgctg gcgcagttta tcttcctgat gctgctgtgg ggggtagcgc
     1261 tgatcgtacg gcggatgccg gggcgcttct cggcgctaat gttgattgtg ctgtcgctga
     1321 ccgtttcttg ccgttatatc tggtggcgtt acacctctac gctgaactgg gacgatccgg
     1381 tcagcctggt gtgcgggctt attctgctct tcgctgaaac gtacgcgtgg attgtgctgg
     1441 tgctcggcta cttccaggta gtatggccgc tgaatcgtca gccggtgcca ttgccgaaag
     1501 atatgtcgct gtggccgtcg gtggatatct ttgtcccgac ttacaacgaa gatctcaacg
     1561 tggtgaaaaa tactatttac gcctcgctgg gtatcgactg gccgaaagac aagctgaaca
     1621 tctggatcct cgatgacggc ggtagggaag agtttcgcca gtttgcgcaa aacgtggggg
     1681 tgaaatatat cgcccgtacc actcatgaac atgcgaaagc gggcaacatc aacaatgcgc
     1741 tgaaatatgc caaaggcgag ttcgtgtcga ttttcgactg cgaccacgta ccaacgcgat
     1801 cgttcctgca aatgaccatg ggctggttcc tgaaagagaa acagctggcg atgatgcaga
     1861 cgccgcacca cttcttctcg ccggacccgt ttgaacgcaa cctggggcgt tttcgtaaaa
     1921 cgccgaacga aggcacgctg ttctatggtc tggtgcagga tggcaacgat atgtgggacg
     1981 ccactttctt ctgcggttcc tgtgcagtaa ttcgtcgcaa accattggat gaaattggcg
     2041 gtattgctgt cgaaacggta accgaagatg cgcacacgtc tctgcgcctg caccgtcgtg
     2101 gctacacctc tgcatatatg cgtattccgc aggcggcggg gctggcgacc gaaagtctgt
     2161 cggcgcatat cggtcagcgt attcgctggg cacgcgggat ggtgcaaatc ttccgtctcg
     2221 ataacccgct taccggtaaa gggctgaaat tcgcccagcg gctgtgctac gtcaacgcca
     2281 tgttccactt cttgtcgggc attccacgac tgatcttcct gactgcgccg ctggcgttcc
     2341 tgctgcttca tgcctacatc atctatgcgc cagcgttgat gatcgccctg ttcgtgctgc
     2401 cgcatatgat ccatgccagc ctgaccaact ccaagatcca gggcaaatat cgccactctt
     2461 tctggagtga aatctacgaa acggtgctgg cgtggtatat cgcaccaccg acgctggtgg
     2521 cgctgattaa cccgcacaaa ggcaaattta acgtcaccgc caaaggtgga ctggtggaag
     2581 aagagtacgt cgactgggtg atctcgcggc cctacatctt ccttgtcctg ctcaacctgg
     2641 tgggcgttgc ggtaggcatc tggcgctact tctatggccc gccaaccgag atgctcaccg
     2701 tggtcgtcag tatggtgtgg gtattctaca acctgattgt tcttggcggc gcagttgcag
     2761 tatcggtaga aagcaaacag gtacgccgat cgcaccgcgt ggagatgacg atgcccgcgg
     2821 caattgcccg cgaagatggt cacctcttct cgtgtaccgt tcaggatttc tccgacggtg
     2881 gtttggggat caagatcaac ggtcaggcgc agattctgga agggcagaaa gtgaatctgt
     2941 tgcttaaacg cggtcagcag gaatacgtct tcccgaccca ggtggcgcgc gtgatgggta
     3001 atgaagttgg gctgaaatta atgccgctca ccacccagca acatatcgat tttgtgcagt
     3061 gtacgtttgc ccgtgcggat acatgggcgc tctggcagga cagctacccg gaagataagc
     3121 cgctggaaag tctgctggat attctgaagc tcggcttccg tggctaccgc catctggcgg
     3181 agtttgcgcc ttcttcggtg aagggcatat tccgtgtgct gacttctctg gtttcctggg
     3241 ttgtatcgtt tattccgcgc cgcccggagc ggagcgaaac ggcacaacca tcggatcagg
     3301 ctttggctca acaatgatga taacgcgatg aaaagaaaac tattctggat ttgtgcagtg
     3361 gctatgggga tgagtgcgtt cccctctttc atgacgcagg cgacgccagc aacgcaacca
     3421 ctgatcaatg ctgagccagc tgtagccgcc cagacggaac aaaatccgca ggtggggcaa
     3481 gtgatgccgg gcgtgcaggg cgctgatgcg ccagtcgtgg cgcagaacgg tccttcgcgt
     3541 gatgtgaagc tgacctttgc gcaaattgcg ccgccgccgg gcagcatggt gctacgtggc
     3601 attaacccga acggcagcat tgagtttggc atgcgcagcg atgaagtggt gacgaaggcg
     3661 atgctcaacc tcgaatacac gccatcgcca tcgttactgc ctgtccagtc gcagttaaag
     3721 gtttatctca atgatgagct gatgggcgtg ctgccagtga ccaaagaaca gttgggtaaa
     3781 aaaacgctgg cgcaaatgcc cattaaccca ctgtttatta ccgacttcaa ccgtgtgcgg
     3841 ctggagtttg tcggccatta tcaggacgtg tgcgaaaacc cggccagcac cacgctttgg
     3901 ctggatgttg ggcggagcag tggactggat ctgacctatc agaccctgaa tgtgaagaat
     3961 gacctgtcac acttcccggt gccattcttt gacccgcgcg ataaccgtac taacaccttg
     4021 ccgatggtct ttgcgggtgc gccggatgtt gggctgcaac aagcctctgc cattgtcgcc
     4081 tcgtggtttg gttcgcgttc tggctggcgt gggcagaact tcccggtgct ctataaccaa
     4141 ctgccggatc gtaatgccat tgtctttgca accaacgaca aacggccgga cttcctgcgc
     4201 gatcatccgg cggtaaaagc ccctgtgatt gagatgatta accatccgca gaatccttac
     4261 gtcaaactgc tggtggtgtt tggtcgtgac gacaaagacc tgttgcaggc agcgaaaggt
     4321 atcgctcagg gtaacattct gttccgtggt gaaagcgtgg tagtgaatga agtgaaaccg
     4381 ctgctaccgc gtaagccgta cgatgcgccg aactgggtac gtaccgatcg tccggtcaca
     4441 tttggcgaac tgaaaaccta tgaagaacag ttacaatcca gcggtcttga gccagcagcg
     4501 attaacgttt cgctaaacct gccgccggat ctctacctga tgcgcagtac cggcattgat
     4561 atggatatta attaccgcta caccatgccg ccggtgaaag acagttcgcg gatggatatc
     4621 agcctgaata accagttcct gcaatccttc aatctgagca gcaaacagga ggcgaaccgc
     4681 ctgctgctgc ggattccggt attacaaggt ttgctggatg gcaaaacaga tgtctctatt
     4741 ccggcgctga aactgggcgc gaccaaccag ctgcgcttcg actttgagta tatgaacccg
     4801 atgccgggcg gttcggtgga taactgtatt accttccagc cggtgcagaa tcatgtggtg
     4861 attggtgacg actccaccat cgacttctcg aagtattacc acttcatccc gatgccggat
     4921 ctacgcgcct ttgctaacgc gggcttccca ttcagccgga tggcggatct gtcgcaaacc
     4981 atcaccgtga tgccgaaagc gcctaacgaa gcacagatgg aaacgttgct gaatactgtt
     5041 ggttttatcg gcgcacagac gggcttcccg gcgattaatc tgacggtgac cgatgatggc
     5101 agcaccattc agggcaaaga tgccgacatc atgatcatcg gtggtatccc ggacaaactg
     5161 aaagacgata agcagatcga cctattggtg caggcgaccg aaagctgggt gaaaacaccg
     5221 atgcgccaga ccccgttccc cggcattgtg ccggacgaga gcgatcgcgc ggcagaaacc
     5281 cggtcaacgc tgacctcttc cggtgcgatg gcggcggtga ttggcttcca gtcgccgtat
     5341 aacgaccagc gcagcgtgat tgcgctgttg gcagatagcc cacgcggtta tgaaatgctt
     5401 aacgatgcgg tgaacgatag cggcaaacgc gccaccatgt tcggttcggt cgcggtgatc
     5461 cgcgagtccg gtatcaacag cctacgtgtt ggcgacgttt attacgtagg tcatctgccg
     5521 tggttcgagc gcgtgtggta tgcgctggca aaccatccga ttctgctggc ggtgctggcg
     5581 gctatcagtg tgatattgct ggcatgggta ctgtggcgtc tgctgcgaat tattagtcgt
     5641 cgtcgtctta acccggataa cgagtaattg aagatgaatg tgttgcgtag tggaatcgtg
     5701 acgatgctgc tgctggctgc ctttagtgtt caggcagcct gtacctggcc tgcctgggag
     5761 cagtttaaaa aggattacat cagtcaggaa gggcgcgtca tcgaccccag cgacgcgcgc
     5821 aaaatcacca cctccgaagg gcaaagttac ggcatgttct ttgccctggc ggctaacgac
     5881 cgtgcagctt tcgataatat tctcgactgg acgcagaaca atctcgctca gggttcttta
     5941 aaagaacgtt tgcccgcctg gctgtggggc aagaaagaga acagtaagtg ggaagtgctg
     6001 gacagcaatt cggcctccga tggtgatatc tggatggcct ggtcgttgct ggaggcgggg
     6061 cgtttgtgga aagagcagcg ttataccgac atcggcagcg cgttgctaaa acgtatcgcg
     6121 cgggaggaag tggtgacggt gcctgggctg ggttccatgt tgttaccggg caaagtgggt
     6181 tttgctgagg ataacagctg gcgttttaac cccagctacc tgccgccgac gctggcgcag
     6241 tatttcaccc gctttggcgc gccgtggacc acgctgcgcg aaaccaatca acgtttattg
     6301 ctggaaaccg ccccgaaagg tttttcgcca gactgggtgc gctatgagaa agacaaaggc
     6361 tggcagctaa aagccgaaaa aacattgatc agcagctacg acgctatccg cgtttacatg
     6421 tgggtaggca tgatgcctga cagcgatccg caaaaagcgc ggatgctcaa ccggtttaaa
     6481 ccgatggcga cattcactga gaaaaacggt tatccgccgg aaaaagtgga tgtggctacg
     6541 gggaaagcgc agggtaaagg accggtcggt ttttctgccg ccatgctgcc ctttttacaa
     6601 aaccgcgatg cgcaggccgt tcagcgccag cgcgtggccg ataactttcc cggcagcgat
     6661 gcctattaca actatgtgct gaccctgttt ggacaaggct gggatcaaca ccgtttccgc
     6721 ttctcgacaa aaggtgagtt attacctgac tggggccagg aatgcgcaaa ttcacactaa
     6781 acatattcac gctttccctc ggtctggccg tcatgccgat ggtcgaggca gcaccaaccg
     6841 ctcagcaaca gttgctggag caagttcggt taggcgaagc gacccatcgt gaagatctgg
     6901 tgcaacagtc gttatatcgg ctggaactta ttgatccgaa taacccggac gtcgttgccg
     6961 cccgtttccg ttctttgtta cgtcagggcg atattgatgg cgcgcaaaaa cagctcgatc
     7021 ggctgtcgca gttagcgccg agttcaaatg cgtataaatc gtcgcggact acgatgctac
     7081 tttccacgcc ggatggtcgt caggcactgc aacaggcacg attgcaggcg acgaccggtc
     7141 atgcagaaga agctgtggcg agttacaaca aactgttcaa cggtgcgccg ccggaaggtg
     7201 acattgctgt cgagtactgg agtacggtgg cgaaaattcc ggctcgccgt ggcgaagcga
     7261 ttaatcagtt aaaacgcatc aatgcggatg caccgggcaa tacgggcctg caaaacaatc
     7321 tggcgctatt gctgtttagt agcgatcgcc gtgacgaagg ttttgccgtc ctggaacaga
     7381 tggcaaaatc gaacgccggg cgcgaagggg cctctaaaat ctggtacggg cagattaaag
     7441 acatgcccgt cagtgatgcc agtgtgtcgg cgctgaaaaa atatctctcg atctttagtg
     7501 atggcgatag cgtggcggct gcgcaatcgc aactggcaga acagcaaaaa cagctggccg
     7561 atcctgcttt ccgcgctcgt gcgcaaggtt tagcggcggt ggactctggt atggcgggta
     7621 aagccattcc cgaactacaa caggcggtgc gggcgaaccc gaaagacagt gaagctctgg
     7681 gggcgctggg ccaggcgtat tctcagaaag gcgatcgcgc caatgcagtg gcgaatctgg
     7741 aaaaagccct cgcactggac ccgcacagca gcaacaacga caaatggaac agtctgctga
     7801 aagtaaaccg ctactggctg gcgatccagc agggcgatgc tgcgctgaaa gccaataatc
     7861 ctgaccgggc agaacgcctg ttccagcagg cgcgtaatgt cgataacacc gacagttatg
     7921 cagtgctggg gctgggcgat gtggcgatgg cgcgaaaaga ttatcccgcc gccgaacgtt
     7981 attatcagca gaccttgcgt atggacagcg gcaacactaa cgccgtgcgc gggctggcaa
     8041 atatttaccg ccagcaatcg ccagaaaaag ctgaagcgtt tatcgcctcg ctctctgcca
     8101 gtcagcggcg tagcattgat gatatcgaac gcagcctgca aaacgaccgt ctggcacagc
     8161 aggcagaggc actggaaaac cagggcaaat gggcgcaggc ggcagcactt cagcggcaac
     8221 gactggcgct ggaccccggc agcgtatgga ttacttaccg actttcgcag gatctctggc
     8281 aggccggaca acgcagccag gccgatacgt taatgcgcaa tctggcgcag cagaagccga
     8341 acgacccgga gcaggtttac gcttacgggc tgtacctctc tggtcatgac caggacagag
     8401 cggcgctggc gcatatcaac agcctgccgc gcgcgcagtg gaacagcaat attcaggagc
     8461 tggttaatcg actgcaaagc gatcaggtgc tggaaaccgc taatcgcctg cgagaaagcg
     8521 gcaaagaagc tgaagcggaa gcgatgctgc gtcagcaacc accttccacg cgtattgacc
     8581 tcacgctggc tgactgggcg cagcagcgac gtgattacac cgccgcccgc gctgcatatc
     8641 agaatgtcct gacgctggag ccaactaacg ccgatgccat tctggggctg acggaagtgg
     8701 atattgctgc cggtgacaca gcggcggcac gtagccagct ggcgaaactg cccgctaccg
     8761 ataacgcctc gctgaacaca cagcggcgcg tggcgctggc gcaggcgcag cttggagata
     8821 ccgcagcggc gcagcagacg tttaataagt tgatcccgca ggcaaaatct cagccaccgt
     8881 cgatggaaag cgcgatggtg ctgcgcgacg gtgcgaagtt tgaagcgcag gcgggcgatc
     8941 caaagcaggc gctggaaacc tacaaagacg ccatggtcgc atccggtgtg actacgacgc
     9001 gtccgcagga taacgacaca ttcacccgac tgacccgtaa cgacgagaaa gatgactggc
     9061 tgaaacgcgg cgtgcgcagc gatgcggcgg acctctatcg ccagcaggat cttaacgtca
     9121 ccctcgagca cgattactgg ggttcgagcg gcaccggtgg ttactccgat ctgaaagcgc
     9181 acactaccat gttgcaggtg gatgcgccgt attctgacgg gcggatgttc tttcgcagtg
     9241 atttcgtcaa tatgaacgtc ggcagtttct ccactaatgc cgatggcaaa tgggatgaca
     9301 actggggcac ctgtacatta caggactgta gcggcaaccg cagccagtcg gattccggtg
     9361 ccagcgtggc ggtcggctgg cgaaatgacg tctggagctg ggatatcggt accacgccga
     9421 tgggcttcaa cgtggtggat gtggtcggcg gcatcagtta cagcgatgat atcgggccgc
     9481 tgggttacac cgttaacgcc caccgtcggc ccatctccag ttctttgctg gcctttggtg
     9541 ggcaaaaaga ctccccgagc aataccggga aaaaatgggg tggcgtacgt gccgacggtg
     9601 tggggctaag tctgagctac gataaaggtg aagcaaacgg cgtctgggca tcgcttagtg
     9661 gcgaccagtt aaccggtaaa aatgtcgaag ataactggcg cgtgcgctgg atgacgggct
     9721 attactataa ggtcattaac cagaacaatc gccgcgtcac aatcggcctg aacaacatga
     9781 tctggcatta cgacaaagat ctgagtggct actcactcgg tcagggcggt tactacagtc
     9841 cgcaggaata cctgtcgttt gccataccgg tgatgtggcg ggagcgcacg gaaaactggt
     9901 cgtgggagct gggggcgtct ggctcgtggt cgcattcacg caccaaaacc atgccgcgtt
     9961 atccgctgat gaatctgatc ccgaccgact ggcaggaaga agctgcgcgg caatccaacg
    10021 atggcggcag cagtcagggc ttcggctaca cggcgcgggc attacttgaa cgacgtgtta
    10081 cttccaactg gtttgttggc acggcaattg atatccagca ggcgaaagat tacgcaccca
    10141 gccatttcct gctctacgta cgttattccg ccgccggatg gcagggtgac atggatttac
    10201 cgccgcagcc gctgatacct tacgccgact ggtaagtttt cagatagcgc ctctcttaat
    10261 gccgctgcga tcgggtatac tcgggcggca atctgggatt tccgggggga gacaatttgc
    10321 gcgtaagtcg ctcgttaaca atcaagcaga tggcaatggt ggcagccgtt gtcctggtgt
    10381 tcgtttttat tttttgcacc gttttgctgt tccatctggt ccaacagaat cgctataaca
    10441 cggctacgca actggaaagc attgctcgct ctgtccgcga acccttatct tcagctattt
    10501 tgaaaggcga tattcccgaa gcggaagcta ttcttgccag cattaaaccg gcaggcgtgg
    10561 tcagccgtgc cgatgtagtg ctgcctaacc agttccaggc gctgcgtaaa agttttattc
    10621 cagagcgccc ggtgccggta atggttactc gcctgtttga gctaccggtt caaatctcgc
    10681 tgggcgttta ctcgctcgaa cgtccggcaa acccgcagcc aattgcctat ctggtactac
    10741 aggcggattc cttccgtatg tataagttcg tgatgagcac cctctcaatg ttagtgacca
    10801 tttacttact tttgtcgctt atcctgaccg tcgccatcag ctggtgcatt aaccgcctga
    10861 ttttgcatcc gttacgcaat attgctcgcg aacttaacgc catcccagcc caggagcttg
    10921 ttggtcacca actggcatta ccgcgtctgc atcaggacga tgaaatcggt atgttggtgc
    10981 gcagttacaa cctcaaccag caattgctgc agcgccatta tgaagaacag aacgaaaatg
    11041 cgatgcgctt cccggtgtcg gatttgccga acaaagcctt gctgatggag atgctggagc
    11101 aggttgtcgc gcgtaaacaa accaccgcgc tgatgatcat cacctgtgaa accctgcgtg
    11161 atactgcggg cgtgctgaaa gaggcgcaac gagaaattct gctgctgacg ctggtggaaa
    11221 aactcaaatc ggtactgtcg ccacgtatga tcctcgcgca gattagcggt tatgactttg
    11281 ctgtcattgc caacggtgta caggaaccgt ggcacgcaat caccttaggt cagcaagtgc
    11341 tcactatcat gagcgagcgc ctgccgattg aacgtattca actccgtccg cactgtagca
    11401 ttggcgtggc gatgttctac ggcgatctca ccgccgaaca gctttacagt cgcgctattt
    11461 ctgcggcatt taccgctcgc cataaaggca agaatcagat tcagttcttt gatccgcagc
    11521 agatggaagc cgcccagaag cggttgacgg aagagagcga tatccttaat gcactggaaa
    11581 atcatcagtt tgctatttgg ttacagccac aggtcgagat gaccagcgga aaactggtca
    11641 gtgcggaagt gttactgcgt atccagcaac cggatggcag ttgggacctg ccggatggct
    11701 taatcgatcg cattgagtgc tgtgggctga tggttaccgt cggtcactgg gtgctggaag
    11761 agtcctgtcg attgcttgca gcctggcaag agcgcggcat tatgctgccc ttgtcggtaa
    11821 acctctctgc gctgcaactg atgcacccaa atatggtggc ggatatgctg gaactgttaa
    11881 cccgctatcg cattcagccg ggaacactga ttctggaagt gacagaaagc cgacgtattg
    11941 acgaccctca tgctgcggtg gcaatcctcc gtccgctgcg caatgccgga gttcgggtgg
    12001 cgctggatga tttcggcatg ggctacgcag ggctgcgtca gctgcagcat atgaaatcgt
    12061 tgccaatcga cgtactgaaa atcgacaaaa tgtttgttga aggcttgccg gaagatagca
    12121 gcatgattgc tgcaattatc atgctggcgc agagcctgaa cttacaaatg attgccgaag
    12181 gcgtggagac tgaagcacaa cgcgactggc tggcaaaagc gggcgttggt attgcccagg
    12241 gcttcctttt tgctcgccca ctccctattg aaatcttcga agagagttac ctggaagaaa
    12301 agtagctacc ccaaactgat tacaaaactt taaaaagtgc tggtttgtgc gagccagctc
    12361 aaacttttta acctttttgt ttcaattatg atccaggtac atttctgtga tgttgtctgg
    12421 gtgttatttt aaggccgcag gtaccccata accttacaag acctgtggtt ttactaaagg
    12481 acaccctatg aaaacctctc tgtttaaaag cctttacttt caggtcctga cagcgatagc
    12541 cattggtatt ctccttggcc atttctatcc tgaaataggc gagcaaatga aaccgcttgg
    12601 cgacggcttc gttaagctca ttaagatgat catcgctcct gtcatctttt gtaccgtcgt
    12661 aacgggcatt gcgggcatgg aaagcatgaa ggcggtcggt cgtaccggcg cagtcgcact
    12721 gctttacttt gaaattgtca gtaccatcgc gctgattatt ggtcttatca tcgttaacgt
    12781 cgtgcagcct ggtgccggaa tgaacgtcga tccggcaacg cttgatgcga aagcggtagc
    12841 ggtttacgcc gatcaggcga aagaccaggg cattgtcgcc ttcattatgg atgtcatccc
    12901 ggcgagcgtc attggcgcat ttgccagcgg taacattctg caggtgctgc tgtttgccgt
    12961 actgtttggt tttgcgctcc accgtctggg cagcaaaggc caactgattt ttaacgtcat
    13021 cgaaagtttc tcgcaggtca tcttcggcat catcaatatg atcatgcgtc tggcgcctat
    13081 tggtgcgttc ggggcaatgg cgtttaccat cggtaaatac ggcgtcggca cactggtgca
    13141 actggggcag ctgattatct gtttctacat tacctgtatc ctgtttgtgg tgctggtatt
    13201 gggttcaatc gctaaagcga ctggtttcag tatcttcaaa tttatccgct acatccgtga
    13261 agaactgctg attgtactgg ggacttcatc ttccgagtcg gcgctgccgc gtatgctcga
    13321 caagatggag aaactcggct gccgcaaatc ggtggtgggg ctggtcatcc cgacaggcta
    13381 ctcgtttaac cttgatggca catcgatata cctgacaatg gcggcggtgt ttatcgccca
    13441 ggccactaac agtcagatgg atatcgtcca ccaaatcacg ctgttaatcg tgttgctgct
    13501 ttcttctaaa ggggcggcag gggtaacggg tagtggcttt atcgtgctgg cggcgacgct
    13561 ctctgcggtg ggccatttgc cggtagcggg tctggcgctg atcctcggta tcgaccgctt
    13621 tatgtcagaa gctcgtgcgc tgactaacct ggtcggtaac ggcgtagcga ccattgtcgt
    13681 tgctaagtgg gtgaaagaac tggaccacaa aaaactggac gatgtgctga ataatcgtgc
    13741 gccggatggc aaaacgcacg aattatcctc ttaatctcat cacttatgcc cgcactccct
    13801 tccccgagtg cgggtgtttg cgcataattg acccctgtgc ctgaacttac cacctgggtg
    13861 agttcaggtt tatttcactt cttccgtgac attttcatgt tcttgcggtc taacacgaag
    13921 tgtttttaac gtcatttaga ctgaccgaca acgggcagtt ttttattacc aggatagttg
    13981 atcaggggtt cacatgcagg gcacaaaaat tcgactttta gcgggcggtt tgctgatgat
    14041 ggccactgct ggctatgtgc aggcagatgc gctccagcct gatccagcat ggcaacaggg
    14101 gacgctttcc aacggtttac agtggcaagt gctgaccacc ccccagcgtc ccagcgatcg
    14161 tgttgaaatt cgcctgctgg ttaataccgg ttcgctcgcc gaaagtacac aacagagcgg
    14221 ttacagtcac gccatccctc gtattgcgct aacgcaaagc ggtggccttg acgcagcaca
    14281 ggcgcgttca ttgtggcagc aggggatcga ccctaaacgc ccgatgccgc cggtaattgt
    14341 ctcttatgac accacgctgt ttaatctgag tttgcccaat aaccgtaacg atttgctgaa
    14401 agaagcgctc tcttatctgg caaatgccac tggcaaattg accatcacac cagaaaccat
    14461 caaccacgcg ctgcaaagtc aggacatggt ggcaacctgg cctgccgata ctaaagaggg
    14521 ctggtggcgc tatcgtctga aagggtcaac cttgttaggt cacgatcctg ccgatccgct
    14581 gaaacaaccc gttgaagcgg aaaaaattaa agatttctat cagaaatggt acaccccgga
    14641 tgcaatgacg ctactggtgg tgggaaacgt ggatgcgcgc tcggttgtcg accaaataaa
    14701 caaaacgttt ggcgaactga aaggcaaacg tgaaacgcca gctccggtgc cgacgctttc
    14761 tccgctgcgt gcggaagcgg tgagtattat gactgacgcg gtgcgtcagg accggttatc
    14821 tatcatgtgg gatacgccgt ggcagccgat tcgtgaatca gccgcactgc tgcgctactg
    14881 gcgtgcggac ctggcccgcg aggcgctgtt ctggcatgtt cagcaagcgt taagcgccag
    14941 taacagcaaa gacatcggtc ttggatttga ctgccgtgtg ctgtatctgc gtgcgcagtg
    15001 tgccatcaac atcgaatcac caaacgacaa gctgaacagc aaccttaatc tggtggcgcg
    15061 tgaactggcg aaggttcgcg ataaaggtct gccggaagaa gagttcaatg cgttagtggc
    15121 gcaaaagaaa ctggagctgc agaaactgtt tgccgcctat gcgcgggctg ataccgatat
    15181 tctgatgggt cagcggatgc gttcgttgca aaatcaggtc gtcgatatcg cgccggagca
    15241 gtatcagaaa ctgcggcagg atttccttaa cagcctgacg gtggagatgt taaatcagga
    15301 tctgcgtcag cagttgtcga atgatatggc gttaatactg ctgcagccga aaggcgagcc
    15361 ggaatttaac atgaaagcgt tgcaggcggc ctgggatcaa atcatggccc catcgactgc
    15421 tgctgccgcc acctctgtcg ccacggatga cgtacatcct gaagtgacgg atattccacc
    15481 tgcacagtaa tgcctgatgg tgatgagtta gcacaaccat caggtattta tggatgagct
    15541 ggatagaggg gtaacacttt atccagcctt ttgcatatgc tgcgtttacg ctggcatcgc
    15601 ctcacgcggg ataatcgcgc cgcgatactg aataacggta cttgcggtca ggtgcccgcg
    15661 tttcgccgcg ttttccgcac tgccgcccgt caaacgtacc gccagataac cggcgctgaa
    15721 agagtcgcca gctgcggtgg tatcgatcac tttttctttc ggcagtttca ccgccggaac
    15781 atccactaac ccttcgccag caatggacac caggcaagaa tccgccccgc gtttcaccac
    15841 cacttctttc acgcccgcgt tatgggtgcg cgcaatgacg tcttccaccg gctgttgacc
    15901 ccacagcgcg tcttcatcgt ccagcgtcag gaaggcgata tccgtgcatt ccagcatttg
    15961 ttggtacacc tgctgtgtct cttctttgct ggcccacagg cgcggacgat agttattgtc
    16021 gaaaatcact tttccgccgt tggcgcggca ttcgcgcagc agggaaagca gcttttcgcg
    16081 gctggtcggg cttaagatcg ccaggctaat cccgctcagg tagagataat cgaaattcgc
    16141 cagctcttcg caaatcgccg cagactgctc actctccagc cagaatttgg cggcggcttc
    16201 gttccgccag tagtagaacg tacgctcgcc ggtgctgtcg gtttcaatgt agtaaaggcc
    16261 cggcagacgg ttttccatcc gttgggtcag ggaagtatca acgttctcgc cgtgccaggc
    16321 gtccagcatt tgctggctaa aactgtccgt tcccagcgcc gttacgtaat gaacggttaa
    16381 tgccgcagga tcgacctgac gggcgatata gacggaagtg ttcagggtat cgccgccgaa
    16441 accgcgctta acgtccgcgc ctttctcgga aagctcaatc atgcattcgc caatcacggc
    16501 aatctttttg gacatagtcg tgaacctgat ctgtaaaaaa ttagcgttag tttgcgctgt
    16561 ggtggtttgc tggtcaacca tattaaaaca gtgttccatt atttttttga gccagaacgt
    16621 attcctgaaa gattccgttg tggagtgagg aaatttaccc cggccaggcg gtaaagttct
    16681 gcccttgcgc gccgataatc tttgtcgagt ccgggcagca tcacttttaa acacaggaca
    16741 tctttgatga taaggcaggt tatccagcga ataagcaacc ctgaagcaag catcgagagc
    16801 ttgcaggaac ggcgtttttg gttgcagtgt gagcgtgctt acacctggca gccgatctat
    16861 caaacatgcg gacggttaat ggccgtggag ctattaacgg tggtcacgca tcccttgaac
    16921 ccttcgcaac gcctgccgcc ggatcgctat tttactgaaa tcaccgtcag ccatcggatg
    16981 gaggttgtga aagagcagat tgatttgctg gcgcaaaaag ccgacttctt tatagagcac
    17041 ggcctgctgg catcggtcaa tattgatggc cctacgctca tcgccctgcg tcagcaacca
    17101 aaaatcctgc gccagattga gcgtcttccc tggctgcgtt tcgaactggt ggagcatatc
    17161 cgactgccga aagattcaac ctttgcctcg atgtgtgaat ttggcccgct gtggctggat
    17221 gattttggta ccgggatggc aaatttctct gcactaagtg aagtgcgtta tgactacatc
    17281 aaaatcgcgc gagaactgtt tgtgatgctg cgtcagtcgc cggaaggacg cacactcttt
    17341 tctcagcttt tacatctaat gaatcgctat tgtcgcgggg tgattgtcga gggcgtagaa
    17401 acgccggaag agtggcgtga tgttcagaac tcgcccgcat tcgccgcaca aggctggttt
    17461 ctttcacgcc cggcaccgat agaaacgctg aatacggcgg ttctggcgct ataagctgcc
    17521 tcattttccg cctggctgga ctatctttag gactggcaca ggaagaatat gaggaagcga
    17581 acgatgagca aggcaggcaa aataaccgct gcgatttcag tggctttctt gttgttgatt
    17641 gtcgtggcga tcattttgat tgcaactttt gactggaatc gactcaaacc gaccatcaac
    17701 cagaaagtct ctgcggagtt gaatcgtccg ttcgctatcc gtggcgatct gggcgtggtg
    17761 tgggagcggc aaaaacaaga aactggctgg cgcagctggg tgccgtggcc ccatgtacac
    17821 gcggaagaca tcattcttgg caatccaccg gatattcccg aagtcacgat ggtgcatttg
    17881 ccacgcgtag aggcaacgct ggccccgctg gcgctgctga ccaaaacggt ctggctgccg
    17941 tggatcaagc tcgaaaagcc cgacgcgcgc ctgattcgcc tctccgaaaa gaacaataac
    18001 tggacgttta atctcgccaa cgatgataac aaagacgcga atgcaaagcc gtcggcatgg
    18061 tcgtttcggc tggataatat tcttttcgat caagggcgga tcgccattga tgacaaagta
    18121 agcaaagcgg atctggagat ttttgttgat ccgttaggca agccgctgcc gttcagcgaa
    18181 gttactggat cgaaaggtaa agcggataaa gaaaaggtgg gcgattacgt ttttggcctg
    18241 aaggcgcagg gacgttataa cggtgaaccg ctcacgggta cgggaaaaat aggcggtatg
    18301 ctggcgctgc gtggcgaagg gacgccgttt ccggtacagg ctgatttccg ctctggtaac
    18361 acccgtgttg cttttgatgg cgtcgtgaat gatccaatga agatgggcgg tgtcgattta
    18421 cggcttaaat tttctggcga ttcactgggt gatctctatg aactgacggg cgttctgctg
    18481 cccgataccc cgccgtttga aacggatggt cggctggtag cgaaaatcga cactgaaaaa
    18541 tcgtcggtct ttgattatcg cggttttaat gggcgaattg gcgatagcga tatccacggt
    18601 tctctggtct acaccaccgg aaagccacga ccaaaactgg aaggtgatgt cgagtcgcgg
    18661 caattgcggc tggcggacct gggaccgtta attggcgttg actccgggaa aggggcagaa
    18721 aagtcgaaac ggtctgaaca gaagaagggc gaaaaaagcg ttcagcctgc gggcaaagtg
    18781 ctgccttatg accgcttcga aaccgataaa tgggacgtta tggatgccga tgttcgcttc
    18841 aaagggcggc gcattgagca tggcagtagc ctgccgatta gtgatctttc tactcatatc
    18901 atcctcaaaa atgctgacct gcgcctgcaa ccgctgaaat ttggcatggc gggcggcagc
    18961 attgcggcga atattcatct ggaaggcgat aaaaagccga tgcaggggcg ggcagatatt
    19021 caggctcgtc gactgaaact gaaagaactg atgcccgatg tggaactgat gcagaagacg
    19081 ctgggggaaa tgaacggtga cgcggaacta cgcggtagcg gtaactcggt ggcggcactt
    19141 ttaggcaaca gtaacggcaa cctgaaactg ttgatgaatg acgggctggt gagccgcaac
    19201 ctgatggaga ttgttgggct gaatgtcggc aactacattg tcggtgcgat atttggtgat
    19261 gatgaggtgc gggtgaactg cgcggcggcg aatctggata ttgccaacgg cgtggcgcgc
    19321 ccgcagatct ttgctttcga tactgagaac gcgttgatta acgttaccgg cacggcaagt
    19381 tttgcttccg aacagctgga tttgactatt gatccggaga gtaaaggaat tcggattatc
    19441 acactgcgtt cgccgctgta tgtgcgtggg acgtttaaaa atccgcaggc tggggtgaaa
    19501 gccgggccgt tgattgcccg tggtgctgtt gctgcggcac tggcaacgct ggtaacaccg
    19561 gcggcggcgt tactggcact gatctcacct tccgaagggg aggctaatca gtgtcggacg
    19621 atattgtcgc agatgaagaa gtgatgaggt tggattgtgt ttgctggtga gttcgggagc
    19681 agcgtgttcc cctcacccta accctctccc caaaggggcg aggggactgt tttgtgcgtg
    19741 ttgaaacaac gagtaccgct tttccgttca ctttctgggg gagggctgtt atgttcgcga
    19801 tataacatct cgctccgctt ttctccctct ccctttcagg gagagggctg gggtgagggt
    19861 ggctgcccac acgatactta caacgactga tgtcgcgtct catgggtcag cagcagggcg
    19921 attaacgtca agccagccat cgccgccaga tataacccca ccgcacctaa cccgtagtta
    19981 gcctgcaacc aggctgcgat atatggtgca acggaagccc cgagaatcga cgctacgttg
    20041 taagagaacg atgctccggt gtaacgcact tctgtcggga acagctctgg taacagcgcg
    20101 cccatcggcc cgaaggtcag tcccatcaga cttaacccca gcagcaggaa ggcaaaaacc
    20161 agaatcgggt tgccagaacc gagcagcggg ttaaaggcga acagcgcgaa caggatgatc
    20221 agcgtggtga tgattaccat gcttttacgg cgaccaaagg catcagccag taatccggcg
    20281 actggcacca tcacgccaaa accaataact gccatcatca gcatccacaa cacttcgtta
    20341 cgcggcaggc caagcccaac tggcgcggcg gcggtactga aggtcataga gtagaccgtc
    20401 atgatgtaaa acagcgtata ggttgccagc ataatgaacg tacccagtac ggttacgcga
    20461 acatgtttgg tcagcagcgt acccagcggg atcttcacct gttttttcgc tttagcgact
    20521 ttctcaaaca ccggcgactc atgcagcgac acgcgaacat acaggccgat aatgaccagc
    20581 accgccgaga agataaatgg cacacgccag ccccagctca taaactgctc gtcggtcagt
    20641 agccaggaaa gcagcaagaa agtgccgttg gcaaagaaga agccaatcgg tgcgcccagc
    20701 tgtgggaagg agccatacag tgcacgtttg cgcggtgggg cgttttcagt cgccagcagc
    20761 gccgcgccgc cccattcacc gcctaagccc agaccctgac caaatcgagc cagcgccagc
    20821 agcagcgggg cgaaaatacc aatcgtggca tagcccggca gcagaccaat caccacggtc
    20881 gaaatcccca tcgttagcaa cgaggcgacc agcgtcgctt tacgcccaac gcgatcgcca
    20941 aaatgaccaa aaacggcaga gccaatgggg cgcgcgacga aggcgatggc gaaggtggcg
    21001 agcgactgta gcgttgctgc cgttggatcg ccctgcggga agaagatatg cggaaacaca
    21061 ataacggccg cagtggcgta aatgtaaaag tcgaagaact caatggccgt gccaatgaga
    21121 gaggcgacaa ggactttatt acgcgagttg atcggcgtgt attcttgctc gtggtcgagt
    21181 gttgtggctg ttgcttgcat aatcttttct tattttttgg ctaacgaata gccatattac
    21241 gcacagcaaa agtgatattt caatgtgtaa caaatcgcgg aaatggtcaa aaaccaggca
    21301 aaaaggggat aatgtttagg ccagtgattt ttcttctatg aaatgcttca cataatttta
    21361 taaatagtgg ataaaactgg tttcaggtta aataaaagtt accaactgga tttatatgct
    21421 ggggttatga gttggaatga aattgtttgc tcccaaatag atggctgaag aggctctggg
    21481 gaagagtttc aagaggttat gtgcgcatta tagccggatg cgatgctggc gcatcttatc
    21541 cggcctacgg ggagccaggc cgaataaaat cgtatgccgg ataaggcgtt tacgccgcat
    21601 ccggcctatt ttaaggctgc gttttccctg gcattcgcgg gtcgtcttta tattcggcgg
    21661 tggcaatcca cgccgcgcaa aacagcgtca aacgggcgaa gaagtagaaa aatgccatca
    21721 gccccagcac ggaaccaaat gctgcgccag agggggattt catcagcgat ggcaaggtgt
    21781 aggtcatcac gattttaatc acttcaaaac caatagcagc cagaaatgtt ccgcgaatca
    21841 gggctttttt gcgcggacgg tggcgcggca accgccagaa gatccagaag aaaagcagat
    21901 agttggcgaa gatggaaatc gccaggccaa tcaaccgcca cgtcggtttc agccactcaa
    21961 tgctgttcag gtgcagggcg ctaataatca tttgctgcgc cgaaccggca accgaggtga
    22021 tcgacagcgt cacaatcagc gcaattaaca aaccaatcag tgaaataaaa tcacgcagat
    22081 attttaccca gaacttctcc tgatcttgcg gcgagcgttc ccagacatcg cgcgactggg
    22141 cacgaatcgc ttcacgcagg ttacccatcc agttgatgcc ggaataaagt gccaccgcca
    22201 ggccgacaag ccctacagtc gtacgctgct gaacggcggt gttgatggtg tttttcaacg
    22261 tggcggctag cgtcggatcg ctgatgtttt gcagaatttt gtcgaagata tcctgtagca
    22321 acatcggatg ggaggccagc acaaagcccc ctgcggcaaa cgacaccatc aaaatcggga
    22381 tcatcgacaa aaatgagaaa taggtgatag ccgcaccaaa ctggttaccc agccgatcgt
    22441 taaagcgttc tgtcgcacga atcaggtgcg caataaccgg ttggcgttgg actttttcgg
    22501 cagtggtggt gacggtttcc agcgcctggc taactttgcc gcctttctcg ctattatcca
    22561 actgtttaat cggctcgtgc tccagatcct gggtgggacg tttgatctcg ttttcctgcg
    22621 tcatccgctc ttttgtcctt ctgtcgtggt cattcttaaa aagtatagtc ggtcagtcca
    22681 ggtgctcttt cattacgcca gccagccact gcataaacag gtttacacgc cgggaaagct
    22741 cccgacgctg cgggtaaacc agcgaaaggg agagcggctc ggcacggtag cctggcaaca
    22801 cttcaataag ccgcccggca cgcagggctt cgcgcacggc aatgcgcggg atctgaataa
    22861 tccccagacc ggcaagaccg gcggcgagat agttttcgct actgtttacc gtcaacatgc
    22921 cgccagactt aaaccactgg acgccattaa cgctggcaac ctcaaaacct aacggatgta
    22981 cacccaggtg cggtgtataa cgcactattg cgtgtgaagt cagatcatcg gggctttgcg
    23041 gataaccaaa gcgcgtcaga tagtgcggac tggcacagtt gaccatggtc agtttgccga
    23101 ggggacgggc gataacgcca tcttccggta acgcgccagt gcgtatcacg caatcaaaac
    23161 catcatgaag aatatctacc ggacggtcat ggctactcag ttccagctca attcccggat
    23221 gcagatagag aaattccgac aggcgcggca gtaacaggct tttcgcgatt cctggcggga
    23281 tgtcgatgcg taatttaccg ctgatactgg tggcatcctg ttgaaacaga ccgtccagtt
    23341 cgctgagatt actcaacaca tcttttgctc gttgataata ggtcatgcct tctggcgtga
    23401 gtttgacccg tcgcgtggtg cgctggagaa gctgggtgcc aagttgatgt tccagtgcct
    23461 gtatctggcg cgaaacactt ccttttggca aagcaaagaa atccgccgcg cgggaaaaac
    23521 tttccagctc cgcgactttg atgaacaact gcattgcgtg aattttatcc atttctttga
    23581 ccgattgttg ttcacagcga aacagtaacg cgcatatcgc attgtttatt gttttttcta
    23641 acatctaatg ccgggtttca caatgttgtc atgacgaatg tggcgatttc tcagcaggaa
    23701 aagggcggaa tagctttctg agaaaggtga atccccctaa acagagcgta tttttcagcg
    23761 attcccttta aagtgaatag aggacgtgtt ggctatttgt gcttattttt gtctcattgc
    23821 cgtttgttat ttttgcttgt ggtcgcggag tttcattttt aattgcatgc gtactaacca
    23881 ccaatataag gaaatcctca aaattgtttt aaatagtgcc ctcgacatgt aatttcaggc
    23941 gtttagctgt aactatttat atcggtaact attttctacg cgtcatctct gtagaatggc
    24001 caccgccaaa tggcgaattc ttattttttc tcgcgggtaa tacccttcca ctacttaagt
    24061 ctttttaacg tattcaggtg gagattcagt tatgcaaata gtcatgtttg acaggcagtc
    24121 aatatttatt catggaatga aaatcagttt acagcagcgt attccaggag tgagtattca
    24181 gggggccagt caggcagacg agttatggca aaagctggaa agttaccctg aagccttagt
    24241 tatgctcgat ggcgatcagg atggtgagtt ttgctactgg ttgctgcaaa aaaccgtggt
    24301 gcagtttcct gaggttaagg tgttaatcac ggcgacggat tgcaataaac ggtggttaca
    24361 ggaagttatc cattttaatg tgctggccat tgtgcctcgt gattcaaccg tcgagacatt
    24421 tgcgttggcg gtaaatagtg cggcgatggg gatgatgttt ctgcctggag actggcggac
    24481 gacgccggaa aaggacatta aagatctcaa atcgttaagc gcccgccagc gggagatttt
    24541 aaccatgtta gcggcgggcg aatcaaataa ggagatcggc agagcgctga atatcagtac
    24601 cggaacggta aaagcgcatc tggaatccct gtatcgccgt ctggaagtga aaaatcgcac
    24661 ccaggcggca atgatgttaa atatctcctc ctgaagagga taattttaca gccagcagga
    24721 agttagcggc tggctgtaaa aatattatgg ttcgccgtac aaaccaatta aacggcgtac
    24781 cacaccgtta gtccagccaa acccatcctg caacggatac tcgccaccgc cgccttcgcg
    24841 gggaacacca tcggcaatat ggtatttctc gatcattttg tgctgttcca gatagaactg
    24901 attcaccgtt ttcagccagc tgcgcgcgat ttcatcaccc agaaggtcat cgccatacat
    24961 tttaaatccc tgaattgcca tccattgtaa cggtgcccag ccattgggtt tatcccactg
    25021 ttcaccggtt tcgtactcgc ttgccagaat cccgccaggt gtcagtaacc ggctgcgtac
    25081 ggcgtttgcc agacgatcgg cctgttcatg gttcgccatg ccgacataga gcggcacaat
    25141 ggcggcagcg gaaaacagcg ccagttgttc gcgtcgccag tcgtaatcgc ggtagatgcc
    25201 gttttcatca tcccagaggt aacggtttac cgcatcgcga cgggcactgg ccttctggcg
    25261 gaacagcgct tctgtctctt tctcgccttt cagcgccgag atgttggcga tggcgctctc
    25321 cagtttaaac aggaaggcat tcagatcgat ggggatgaac tgggtggtac gaatgctcgc
    25381 cagacgacca gtatcacgca gccaacggga agagtaatcc caacctgagg ccgctcccgc
    25441 gcgtaaatcg cggtacacct cgttgggcgg gcgaccagaa tgtttcgcgg tttcaacgtc
    25501 ctcaagccag gattcgtcac gcggcgtgtc gcgatcgtcc caataacggt tgagcagcga
    25561 tccgtccggc atccgcacaa catggcgata ggcctgatta gggatcaacg attctgcacc
    25621 gtccatccag aaggcatatt ccattttaag gtggtcgaga tagcggcgcg caccgcgtac
    25681 accatcttct tcaaacaact ccaccatcag cgcaaaaacc ggtggttgcg atcggctcaa
    25741 ataataggtg cggttgccgt tggggatgtg accatagttt tcgatcatcc aggcgaagtt
    25801 atcggccatg catttcagca aatcttcccg accactttcc gccagcccca gcatggtgaa
    25861 ataggaatcc caatagtagg tttcgctaaa acggccgccc gggacaatat atgactgcgg
    25921 tagcgccagc agagaagacc acggaatgtg atcctgtggt tcgcgggtta gcaccggcca
    25981 cagctggtcg atatgctctt tcagggaatt ttgcgggtcc gatacatact cgctggagta
    26041 gacctccggc agccagaagt gattttcaac aaacttgcgc aagtcaaaat cacgatgacg
    26101 gcgcacttta cggtagcgga ttaaaatatc cagcgggtcc attttgggtg cgcagtcggg
    26161 aaaggtttta ctgtcgggga aaattttcgc cgactgaaca tgctcgaaca gttcgagata
    26221 gcgatcggca ggcgtcagcg catcagaggc gggcagccct tcaatcatct cgggttccgg
    26281 ttctgcctcg atcatctcat ccagttttaa ttcatacggg tccagttcat agcagagatc
    26341 gacctcgatc atcagttcgt ctggattagg gttttgaatt ttctgattga gcataaggat
    26401 ccaacctccg aaattcgtcg ctaattatcg ggcttttctt taagggtagt cattcgctcc
    26461 gcctggtggg cgacaaagcg tgcggtggat cacgtttatt aacttgtcaa aaaatataac
    26521 gcactaatat caatggaatt tataaaaata cggatttcgt gttgtttttc attttcagaa
    26581 cattcaatcc tgctctcgcc gcaatcatga tatgccattc ctgatctggg taataggcac
    26641 aggctatctt attgatagtt tatattcatg taattgattg attcctatca aaaagcgtat
    26701 ctccctgttt tttatgatgg ctttgtctcg cagagacgtt tgcagcattc tggaaaattt
    26761 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
    26821 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
    26881 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
    26941 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
    27001 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
    27061 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
    27121 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
    27181 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
    27241 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
    27301 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
    27361 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
    27421 ctcattgcca tgcgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
    27481 gtggcgcagt tgggccgatt aacgcgccga cggtatttaa ctcagtattt aacgttgagc
    27541 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
    27601 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
    27661 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
    27721 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
    27781 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
    27841 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
    27901 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
    27961 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
    28021 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
    28081 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
    28141 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
    28201 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
    28261 aatat
//
LOCUS       DAZGLJ010000039        28130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000039 DAZGLJ010000000
VERSION     DAZGLJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.039"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..405)
                     /locus_tag="ACHK49_001514"
     CDS             complement(<1..405)
                     /locus_tag="ACHK49_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO9262184.1"
                     /translation="MNSNYMPFTQRDSLGRYYTKESISALLVSQMKAEKVNNIIDLAS
                     GEGSLTYAALDRWKNAEAYSLDIESRMSKKVCDNLTHIVTDALVHSFPEMLARHQGNF
                     DVAVCNPPFTLPEWRDDYFKIISEIGADKYISV"
     gene            complement(647..1855)
                     /locus_tag="ACHK49_001515"
     CDS             complement(647..1855)
                     /locus_tag="ACHK49_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9262185.1"
                     /translation="MKLNARQVDAAKPREKAYKLADGAGLYLEVVPSGSRYWRMKYRF
                     NGKEKRMAFGVYPAVSLAQARALRDDAKKKLAEGIDPSLAKKEEKLVRDVQLNNTFQA
                     VALEWHGTKVSRWSEGYASDIIEAFNKDIFPYIGQQPVNEIKPLVLLNVLRRIESRGA
                     TEKAKKVRQRCSEVFRYAIVTGRAEYNPAADLTSAMSGHESKHYPFLTVEELPDFFKA
                     LAGYTGSPLVVLAARLLILTGVRTGELRGAFWSEFDLEKAVWEIPAERMKMKRPHLVP
                     LSTQALEIVQQLKVMSGQYPLVFPGRNDPRKTMSEASINQVFKRIGYTGRVTGHGFRH
                     TMSTILHEEGFNTAWIETQLAHVDKNAIRGTYNHALYLEGRREMMQWYADYINANHNS
                     CISLLVNATK"
     gene            complement(2019..2094)
                     /locus_tag="ACHK49_001516"
     tRNA            complement(2019..2094)
                     /locus_tag="ACHK49_001516"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2059..2061),aa:Thr,seq:cgt)
     gene            complement(2209..3462)
                     /gene="proA"
                     /locus_tag="ACHK49_001517"
     CDS             complement(2209..3462)
                     /gene="proA"
                     /locus_tag="ACHK49_001517"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO9262186.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(3474..4577)
                     /gene="proB"
                     /locus_tag="ACHK49_001518"
     CDS             complement(3474..4577)
                     /gene="proB"
                     /locus_tag="ACHK49_001518"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO9262187.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            4865..5920
                     /gene="phoE"
                     /locus_tag="ACHK49_001519"
     CDS             4865..5920
                     /gene="phoE"
                     /locus_tag="ACHK49_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO9262188.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNFYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(5959..6360)
                     /gene="crl"
                     /locus_tag="ACHK49_001520"
     CDS             complement(5959..6360)
                     /gene="crl"
                     /locus_tag="ACHK49_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO9262189.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6418..7662)
                     /gene="frsA"
                     /locus_tag="ACHK49_001521"
     CDS             complement(6418..7662)
                     /gene="frsA"
                     /locus_tag="ACHK49_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO9262190.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(7754..8212)
                     /gene="gpt"
                     /locus_tag="ACHK49_001522"
     CDS             complement(7754..8212)
                     /gene="gpt"
                     /locus_tag="ACHK49_001522"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO9262191.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            8473..9930
                     /gene="pepD"
                     /locus_tag="ACHK49_001523"
     CDS             8473..9930
                     /gene="pepD"
                     /locus_tag="ACHK49_001523"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO9262192.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(9987..10601)
                     /gene="prfH"
                     /locus_tag="ACHK49_001524"
     CDS             complement(9987..10601)
                     /gene="prfH"
                     /locus_tag="ACHK49_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO9262193.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSLRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(10598..11737)
                     /locus_tag="ACHK49_001525"
     CDS             complement(10598..11737)
                     /locus_tag="ACHK49_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO9262194.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(11983..12435)
                     /locus_tag="ACHK49_001526"
     CDS             complement(11983..12435)
                     /locus_tag="ACHK49_001526"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9262195.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(12432..13487)
                     /gene="dinB"
                     /locus_tag="ACHK49_001527"
     CDS             complement(12432..13487)
                     /gene="dinB"
                     /locus_tag="ACHK49_001527"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO9262196.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(13558..14481)
                     /gene="lafU"
                     /locus_tag="ACHK49_001528"
     CDS             complement(13558..14481)
                     /gene="lafU"
                     /locus_tag="ACHK49_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO9262197.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            complement(14484..15347)
                     /gene="motA"
                     /locus_tag="ACHK49_001529"
     CDS             complement(14484..15347)
                     /gene="motA"
                     /locus_tag="ACHK49_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO9262198.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(15360..16076)
                     /locus_tag="ACHK49_001530"
     CDS             complement(15360..16076)
                     /locus_tag="ACHK49_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HFO9262199.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLVGRELEDQFVTQQMLQQALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(16096..16563)
                     /locus_tag="ACHK49_001531"
     CDS             complement(16096..16563)
                     /locus_tag="ACHK49_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HFO9262200.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            complement(16571..17632)
                     /locus_tag="ACHK49_001532"
     CDS             complement(16571..17632)
                     /locus_tag="ACHK49_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO9262201.1"
                     /translation="MNPTLLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRQTQQQELTPLPPQLQELISQLPQAKPEQQARLATYASEDLHAITPTQ
                     PRVSTQPARPKSELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELLSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(17629..17940)
                     /locus_tag="ACHK49_001533"
     CDS             complement(17629..17940)
                     /locus_tag="ACHK49_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HFO9262202.1"
                     /translation="MEKQRRKLFALVEAMNEALDKQRWRRLPALHQQLIRQFHDYAAA
                     ETDAAQLDAVKARLYDAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(17945..18337)
                     /gene="fliS"
                     /locus_tag="ACHK49_001534"
     CDS             complement(17945..18337)
                     /gene="fliS"
                     /locus_tag="ACHK49_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO9262203.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(18360..19676)
                     /gene="fliD"
                     /locus_tag="ACHK49_001535"
     CDS             complement(18360..19676)
                     /gene="fliD"
                     /locus_tag="ACHK49_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO9262204.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQTELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSASVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ATGPEITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSTSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(19883..20797)
                     /gene="lafA"
                     /locus_tag="ACHK49_001536"
     CDS             complement(19883..20797)
                     /gene="lafA"
                     /locus_tag="ACHK49_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HFO9262205.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            21283..22125
                     /locus_tag="ACHK49_001537"
     CDS             21283..22125
                     /locus_tag="ACHK49_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFO9262206.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLEKLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSLPDSSPAKKLIITDQIQETVPEEISSTTPPVPVRKKHKVIMGLALAAAVIFIGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNSVPLLSQTLAPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     LQKC"
     gene            complement(22180..23157)
                     /locus_tag="ACHK49_001538"
     CDS             complement(22180..23157)
                     /locus_tag="ACHK49_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HFO9262207.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVAPTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQNTALMQML
                     DKRTALSGGAVDRQLQPVLQGEARVTFHSPDLANLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAAQYQTRLTNALRRVGVQLHQQADGISFSTTEKQWPHIERTLSVRTDGDK
                     SAFMPLKTFAEPSEADRLAQSLQQGGAGISQTLESINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQVLLQAVNGQARISSQTVRNLLG"
     gene            complement(23174..24103)
                     /gene="flgL"
                     /locus_tag="ACHK49_001539"
     CDS             complement(23174..24103)
                     /gene="flgL"
                     /locus_tag="ACHK49_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO9262208.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGDLAHVVELMATGKQILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLAVRDDLLEAANGTN
                     TADSLASLGQDIESLTESMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEVTLKTYSKVSELNLFSVL"
     gene            complement(24118..25494)
                     /gene="flgK"
                     /locus_tag="ACHK49_001540"
     CDS             complement(24118..25494)
                     /gene="flgK"
                     /locus_tag="ACHK49_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO9262209.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGDLSQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTSTMSLTFAGTTSTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMASQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PNITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSAAQNQQSSVSGVSMDEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
     gene            complement(25676..25975)
                     /locus_tag="ACHK49_001541"
     CDS             complement(25676..25975)
                     /locus_tag="ACHK49_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HFO9262210.1"
                     /translation="MKVNASGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMIVKQLSPKHK"
     gene            complement(25975..27075)
                     /locus_tag="ACHK49_001542"
     CDS             complement(25975..27075)
                     /locus_tag="ACHK49_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO9262211.1"
                     /translation="MQNWIKAAVIAVSLALPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVTVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(27090..27755)
                     /gene="flgH"
                     /locus_tag="ACHK49_001543"
     CDS             complement(27090..27755)
                     /gene="flgH"
                     /locus_tag="ACHK49_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO9262212.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPKTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FSHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADACISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(27838..>28130)
                     /locus_tag="ACHK49_001544"
     CDS             complement(27838..>28130)
                     /locus_tag="ACHK49_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HFO9262213.1"
                     /translation="DVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGED
                     GLGTLQDNALEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
BASE COUNT         6466 a         7585 c         7070 g         7009 t
ORIGIN      
        1 gacagatata tatttatctg cgccaatctc actaatgatt ttaaaataat catccctcca
       61 ttcagggaga gtgaatggag gattacatac tgcaacatca aaatttcctt gatgtcgggc
      121 aagcatttca ggaaatgaat gaacaagggc atctgttaca atatgagtaa gattatcaca
      181 tacttttttt gacatccgag attctatatc caaagaatat gcttctgcat tcttccatcg
      241 gtctaaagca gcataggtta agctcccctc accagaagct aaatcaataa tattattgac
      301 tttttcagct ttcatctgag aaaccaataa tgcacttatg gattcttttg tatagtatct
      361 tccgagacta tctcgttgtg tgaatggcat atagttactg ttcatttttt atactcatct
      421 taagtacgct accgccctag gcgttacggc tacccaagaa ccaacacaat catgtaatct
      481 attgatattt ataaacttac cttcaaatct ttcagcccta gaacctaaaa aagatagaag
      541 tgtaggtaac aaagggcgat cggatatagg gctaatgttt aaacttaagt atcaaaatat
      601 acggttttag gtcattttcc taatcaatcc tatcatcacg tcaaatctac tttgtagcgt
      661 tcaccagcaa agaaatacat gaattatgat tagcgtttat atagtccgca taccactgca
      721 tcatctccct acgtccttcc aaatacaacg catggttata cgtcccacga atcgcatttt
      781 tatcaacgtg agccagctgg gtttcaatcc acgctgtatt gaaaccttcc tcatgcaaaa
      841 tcgtactcat cgtgtgacgg aaaccatgcc ccgttaccct ccccgtatat ccaatccgct
      901 taaaaacctg attaatactc gcttcactca tcgtcttgcg gggatcatta cgtcccggga
      961 acaccagtgg atattgccct gacatcactt tgagttgctg tacgatttcc agcgcttggg
     1021 tagagagggg gacaaggtga ggccgtttca tcttcatacg ctcggcaggt atctcccaca
     1081 ccgctttttc aagatcaaac tcactccaga aagcacctcg gagttcgcca gtgcgaactc
     1141 ccgtgaggat cagcagacgt gccgcaagaa caactaacgg gcttcctgtg tagcctgcga
     1201 gagctttaaa aaagtctggt aactcctcaa cagtaaggaa gggataatgc ttcgattcat
     1261 gccctgacat cgcgctggta agatccgctg caggattgta ttccgcacga ccggttacga
     1321 tggcgtaacg aaagacttcg ctgcaacgct ggcgaacctt cttggccttc tctgtcgcgc
     1381 cacggctttc aattcgacgc agcacattaa gcagaaccag cggtttgatt tcattcaccg
     1441 gttgttggcc aatataggga aaaatatctt tattaaaggc ttcgataatg tccgaggcat
     1501 aaccttctga ccatcggctc accttcgttc catgccattc aagtgccaca gcctgaaacg
     1561 tattatttag ctgcacatct cgaaccagct tttcttcttt cttggcaagc gatggatcga
     1621 taccttcggc aagctttttc ttggcgtcgt cacgcagtgc cctcgcttgt gcaagggaca
     1681 ctgccggata gacaccaaag gccatacgct tctcttttcc attgaagcga tatttcatcc
     1741 gccagtatct ggaaccagag ggaacaacct caagatacaa accagcacca tctgccagct
     1801 tataggcttt ctctctgggt ttagctgcgt ctacctgtcg cgcattaagt ttcattgggg
     1861 gcatctccct agaccgaaca caaaatgccc ccacttatgc ccccaactgc gacttgattt
     1921 cggttgagtc tagttgataa caggagataa gatacgggcc agaaaccgca gtatacgagc
     1981 ttttagttga tttcggtaga cttgggaaga gtttgaaatg gtgccgataa taggagtcga
     2041 acctacgacc ttcgcattac gaatgcgctg ctctaccaac tgagctatat cggccctgaa
     2101 aggacatgtt cacgaacgtg aatcacggtg gacaaggtta aaactaaccg ggcgatgcgt
     2161 caatggcctt gtgaatcaaa tggctacttt tgcatcaccc ggttttattt acgcacgaat
     2221 ggtgtaatca ccaatgccga tccacttgta agtggtcagt gcttccagcc ccattgggcc
     2281 acgcgcgtgg agtttttgtg tgcttaccgc cacttccgca cccagaccaa actggccgcc
     2341 gtcggtaaaa cgcgtagagg cgttaacgta aacagcggac gaatccactt cgttaacaaa
     2401 acgctgggcg ttgcgcatat cgcgggtcag gatcgcatcg gagtgttgtg tgccgtgttc
     2461 acgaatatgg gcgatggcat cgtcaagatc gctgacgatt ttgacgttca aatctaatga
     2521 cagaaactca tcgtcatact cttcggcttt aacagcaacc accttcgcag ggcctgcctg
     2581 caactgcgcc agtgcagctg catctgcgtg taatgtcacg ccgctttccg ccatttgttt
     2641 gcttaatgcg ggcaggaagc tatcggcgat gtttttattc accagcaacg tttcaaccgt
     2701 attacatgtg ctcggacgct gagttttcgc gttgacgatc acttttaatg cttcagcgat
     2761 ctctacactt tcatcaacgt aaatatggca tacgcctata ccacctgtga tcaccgggat
     2821 tgtcgactgt tcacggcaca gtttatgcaa accagcgcca ccacgcggga tcagcatgtc
     2881 gatgtattta tccatacgca gcatttcact gaccagcgca cggtcaggat tatcaatcgc
     2941 ctgcacggca cccgccggta agccgcagga tttcagggcg tcctgaatca ccgccaccgt
     3001 tgcagcgtta gtgcgacacg tttctttgcc accgcgcagg atcaccgcat taccggtttt
     3061 caggcacagc gaagcgacat caaccgtcac gttcgggcgc gcttcataaa tcacgccaat
     3121 aacccccagc ggtacgcgac gacgctcaag acgcaggccg ctgtccagta cgctgccatc
     3181 gattacctgc cccaccggat cggcgaggtt acacacctgg cgcacatcat cggcaatgcc
     3241 tttcagccgt gcgggcgtca gtgccagacg gtcaagcatc gcttcgccaa ggccattggc
     3301 acgcgcgtca gcaacatcct gggcgttagc gttgaggatg atttcgcttt gtgcttccag
     3361 ttcatcggcg attttttcca gcacgcgatt tttttcgcgg ctggagagtt gcgctaattt
     3421 atacgaggct tgcttcgcgg caatgcccat ttgttccagc atcagcctgc tccttaacgg
     3481 gtaatcatgt catcacggtg aacggcaacc gggccgtatt catatcccag tattgcatca
     3541 atttcttgcg agtggtgccc ggcaatacgg cgtaatgcat cgctgttgta acgactgacg
     3601 ccgtgggcga tatcgcgacc ttcgaggttg caaatgcgga tgacttcacc acgcgagaaa
     3661 ttgccagtca cgcttttaat gcctttcggc aacagggagc tgccgcgttc aagaatggcg
     3721 gcagttgccc cttcatctac cgtgatttca cccgccggcg gcgcaccgaa aatccagcgt
     3781 ttacggtttt caagcggagt cgcctgggca tggaacagcg taccgacgga aatgccttcc
     3841 atcacatcac caataacgcc cggcttgctg cccgcggcaa taatggtgtc gatacccgca
     3901 cggcaagcca cgtcagcagc ctgcaatttg gtactcatgc cgccagttcc gaggcctgaa
     3961 acgctgtcac cggcaatcgc gcgcagtgcg tcatcaatgc cgtaaacatc tttaatcagt
     4021 tctgcctgcg gattgctgcg cgggtcagcg gtatacaaac ctttttgatc ggtcagcagc
     4081 aacagtttat cggcacccgc cagaatcgcc gccagcgcag aaaggttatc gttatcgccg
     4141 actttaatct ctgccgtagc gacagcatcg ttctcattga ttaccggaac gatattgtta
     4201 tcgagcaacg cacgcagggt gtcgcgggcg ttcaggaagc gttcacggtc ttccatatca
     4261 gcacgggtca gcagcatttg cccgacgtga atgccataaa tcgaaaacag ctgttcccac
     4321 agttgaatca gtcgactctg ccctaccgct gccagcagtt gtttcgaggc gatagtcgct
     4381 ggcagttccg ggtaacccag gtgctcacgt ccggcggcga tcgcgcccga cgtcacaata
     4441 acaatccgat gcccggcggc atgtaactgc gcgcactggc gaacaagttc aacgatatgg
     4501 gcacggttca gacggcgcga tccgcctgtt agcacactgg tgccgagttt taccaccagc
     4561 gtctggctgt cactcatgat tctctgccat tcaattttag gaaaaatgat atcaaacgaa
     4621 cgttttagca ggactgtcgt cggttgccaa ccatctgcga gcaaagcatg gcgttttgtt
     4681 gcgcgggatc agcaagccta gcggcagttg tttacgcttt tattacagat ttaataaatt
     4741 accacatttt aagaatatta ttaatctgta atatatcttt aacaatctca ggttaaaaac
     4801 tttcctgttt tcaacggggc tctcccgctg aatattcgcg cgttaattaa aatcaggaat
     4861 gaaaatgaaa aagagcactc tggcattagt ggtgatgggc attgtggcat ctgcatctgt
     4921 acaggctgca gaaatatata ataaagacgg taataaactg gatgtctatg gcaaagttaa
     4981 agccatgcat tatatgagtg ataacgccag taaagatggc gaccagagtt atatccgttt
     5041 tggttttaaa ggtgaaacac aaattaacga tcaactgact ggttatggtc ggtgggaagc
     5101 ggagtttgcc ggaaataaag cggagagtga taccgcacag caaaaaacgc gtctcgcttt
     5161 tgccgggttg aaatataaag atttgggttc tttcgattat ggtcgtaacc tgggcgcgtt
     5221 gtatgacgtg gaagcctgga ccgatatgtt cccggaattt ggtggcgatt cctcggcgca
     5281 gaccgacaac tttatgacca aacgcgccag cggtctggcg acgtatcgga acaccgactt
     5341 cttcggcgtt atcgatggcc tgaacttaac cctgcaatat caagggaaaa acgaaaaccg
     5401 cgacgttaaa aagcaaaacg gcgatggctt cggcacgtca ttgacatatg actttggcgg
     5461 cagcgatttc gccattagtg gggcctatac caactcagat cgcaccaacg agcagaacct
     5521 gcaaagccgt ggcacaggca agcgtgcaga agcatgggct acaggtctga aatacgatgc
     5581 caataatttt tatctggcaa ctttttattc tgaaacacgc aaaatgacgc caataactgg
     5641 cggctttgcc aataagacac agaactttga agcggtcgct caataccagt ttgactttgg
     5701 tctgcgtcca tcgctgggtt atgtcttatc gaaagggaaa gatattgaag gtatcggtga
     5761 tgaagatctg gtcaattata tcgatgtcgg ggctacatat tatttcaaca aaaatatgtc
     5821 agcgtttgtt gattataaaa tcaaccaact ggatagcgat aacaaattga atattaataa
     5881 tgatgatatt gtcgcggttg gcatgaccta tcagttttaa tgattattgc cggatgtgat
     5941 gcatccggca catttcactc acgccgttaa cttcaccggc tcgtcacgaa aatcatccgc
     6001 cggttccagc ttcagattca gcgtcgtcag cagttcacgc agcttctcgt gaaactcacg
     6061 cagggtgtgc tccagtcgtt caaccacttc agtgtctttt accggaacac tcttccagtc
     6121 gcctgcttta tcgaacagac caaactggta actgtaggta aaacgggatt cctgcgcttc
     6181 aagctccatc caccagcccc agaactcacg cacttccggt gccggtttca cgttgacgca
     6241 tacagccaga caatcgaaaa agaatcgatt atcttcgcac ttaccttcac gaatatacgg
     6301 gcctagtgcg gtaaattttt tgatcaatct gctcttcggg tgtccactcg gtaacgtcat
     6361 tgctatctcc tgttgtgatg caactgtttt accaaattgg caaaatttag caaattttta
     6421 acacaagcgt ttttcgatcc agccggtgat ttcctgaaga cctttgtcaa aattccgata
     6481 caccgggtta aatgggatct ctaataattt accgtcagca gatgatgagg tgattaagcg
     6541 tgagtcctct tccgggctga acggatcgtt cttccagtag cctgataaca ttggcgttgg
     6601 gcagcgacgc cccagcaatc cttgcacttt taatgaatag cgattcagct ccacgcgtaa
     6661 cgcgtcatcg gaagcatcat gcatccccaa acgactcgcc agaacgtcaa gatacatttc
     6721 cggcacctgt tgctggcact taaaatcact caacagggta tgaactaccg gaccaagaca
     6781 ggcaaccgct ttcagacgcg gcgattcaag atatgccaga cgcacggcaa cgttagcgcc
     6841 gaaacggaaa ccaaaggccg cgacgcgagt gtgatccacc cacggtacgt taggcagcgc
     6901 ctttaagacg tgctgatgca acaggctgga gtcctgggtg agcttccatt ttgaagaaaa
     6961 gcccaccgat ggcatatcaa tagtcagcat cgcaatgccg cgcggcgcaa aataacgttc
     7021 atacaggctg taatagtccg tctgcatcgc atccagacca ccacacatta atactgtcgg
     7081 gaacgggcca tcgcctttcg gcatatgcaa aaagccggtg atgggcgcac cgccgggtac
     7141 ggtaaactcc atctgccgca tcgtgcccgg cagacgctgg gcggcctctt cataggcgcg
     7201 gtttgataaa gcctgcgctt gctcggccag gtcatctcct ttcagatgag gataggcggc
     7261 aatgttgtac aacgtagccg catgcagcca gtgtcgacca ctgagttgcg gatcttcttc
     7321 cgcacaggct ttttgttgcc acaccatcgc ctgggtggcc cactcataaa tccagttgcc
     7381 accacggtag ccaatcacag tatcgtataa atcatcgtcg gtacgttcag catcgctcat
     7441 cacaatacgt gcctggacgt cgaggatttc acgtgggtca ataccgcgcc agatccacat
     7501 cagacggtta atcatgcgat accagtgagg gatggtttta ccatcaagag ccgactgcac
     7561 aggcggttgt gcgccgtgat taaagcggcg gactagcgtc gaggtttcag gatgtttaaa
     7621 gcggggtttg aacagggttt cgctcaggtt tgcctgtgtc atggatgcag cctccagaat
     7681 acttactgga aactattgta acctgcctga agttaaaaag aacaacgccc ggcagtgcca
     7741 ggcgttgaaa agattagcga ccggagattg gcgggacgaa tacgacgccc atatcccacg
     7801 gctgttcgat ccaggtatct tgcgggatat caacaacata gtcatcaacc agcggacgac
     7861 cagccggttt tgcgaagatg gtgacaaagt gcgcttttgg atacatttca cgaatcgcaa
     7921 cagcagtacc accggtatcc accaggtcat caataacgat gaagccttcg ccatcgcctt
     7981 ctgcgcgttt cagcacttta agctcgcgct ggttgtcgtg atcgtagctg gaaatacaaa
     8041 cggtatcgac atgacgaata cccagttcac gcgccagtaa cgcacccggt accagaccgc
     8101 cacggcttac ggcaataatg cctttccatt gttcagaagg catcagtcgg cttgcgagtt
     8161 tacgtgcatg gatctgcaac atgtcccagg tgacgatgta tttttcgctc atgtgaagtg
     8221 tcccagcctg tttatctacg gcttaaaaag tgttcgaggg gaaaataggt tgcgcgagat
     8281 tatagagatc tggcgcacta aaaaccagta tttcacatga gtccgcgtct ttttacgcac
     8341 tgcctctccc tgacgcggga taaagtggta ttctcaaaca tatctcgcaa gcctgtcttg
     8401 tgttgacaac attttctgct aaccctgtga cctgcaatac tgttttgcgg gtgatcgaca
     8461 aggagactta acgtgtctga actgtctcaa ttatctccac agccgctgtg ggatattttt
     8521 gccaaaatct gttctattcc tcacccgtcc tatcatgaag agcaactcgc tgaatacatt
     8581 gttggttggg caaaagagaa aggtttccat gtcgaacgcg atcaggtagg taatatcctg
     8641 attcgtaaac ctgctaccgc aggtatggaa aatcgtaaac cggtcgtctt acaggcccac
     8701 ctcgatatgg tgccgcagaa aaataacgac accgtgcatg acttcacgaa agatcctatc
     8761 cagccttata ttgatggcga atgggttaaa gcgcgcggca ccacgctggg tgcagataac
     8821 ggcattggta tggcgtctgc gctggcggtt ctggctgacg aaaacgtggt tcacggcccg
     8881 ctggaagtgc tgctgaccat gaccgaagaa gccggtatgg acggtgcgtt cggcttacag
     8941 agcaactggt tgcaggctga tattctgatt aacaccgact ccgaagaaga aggtgaaatc
     9001 tacatgggtt gtgcgggggg tatcgacttc acctccaacc tgcatttaga tcgtgaagcg
     9061 gttccagctg gttttgaaac cttcaagtta accttaaaag gtctgaaagg cggtcactcc
     9121 ggcggtgaaa tccacgttgg cctgggtaat gccaacaaac tactggtgcg cttcctggcg
     9181 ggtcatgcgg aagaactgga tctgcgcctt atcgatttca acggcggcac actgcgtaac
     9241 gccatcccgc gtgaagcctt tgcgaccatt gctgtcgcag ctgataaagt cgacgtcctg
     9301 aaatctctgg tgaataccta tcaggagatc ctgaaaaacg agctggcaga gaaagagaaa
     9361 aatctggcct tgttgctgga ctctgtagcg aacgataaag ctgccctgat tgcgaaatct
     9421 cgcgatacct ttattcgtct gctgaacgcc accccgaacg gtgtgatccg caattccgac
     9481 gtggcaaaag gtgtggtcga aacctccctg aacgtcggtg tggtgaccat gactgacaat
     9541 aacgtagaaa ttcactgcct gatccgttca ctgatcgaca gcggtaaaga ctacgtggtg
     9601 agcatgctgg attcgctggg taaactagct ggcgcgaaaa ccgaagcgaa aggcgcatat
     9661 cctggctggc agccggacgc taattctccg gtgatgcatc tggtacgtga aacctatcag
     9721 cgtctgttca acaaaacacc gaacatccag attatccacg cgggcctgga atgtggtctg
     9781 ttcaaaaaac cgtatccgga aatggacatg gtttctatcg ggccaactat caccggtcca
     9841 cactctccgg atgagcaagt tcacatcgaa agcgtaggtc attactggac gctgctgact
     9901 gaactgctga aagaaattcc ggcgaagtaa ttatttgatt tgctgccgga tggcgtttaa
     9961 tcgccttccg gcagtttcat ccttcattat ccttcgataa aagccatccc tgtaaatgtc
    10021 cgtctcgggt tgccacgttc aatctggtga tggaacattc gccgcagcga tttcagcacc
    10081 gcgctatttt cctgttgctg ctgctccagc ttccaggcaa tcagcaatcg tgccagccgc
    10141 ttgttagcat gctgactacg ctctgactga accttcacgc taataccgga tgccaaatgc
    10201 gtggcgcgta ccgccgagtc ggttttattg acatgttgac cgcccggccc cgacgaacgc
    10261 aacgtctcat aacggattgc atccgattgt tcctgctcat cagcggtaaa acgcccaatg
    10321 cccagaaacc agtttttgcg cccatgatga ggccgatacg gactcggaca aatccactga
    10381 atagtgccac accagctttc gcttaatgcc catgcgttat cgccatccag agaaatcagc
    10441 gccgaacgca gcgtgtcaga gtagtggccc gtttctgttt ccagcaccgt taccgcgacg
    10501 tcttgtcggg cagcttcttt aattagcctg tccagcgcct ttttcactgc gagacaacat
    10561 tcttccggcc cctgcgctga ggagagttgt agcaagatca tccttttttc cctccactgt
    10621 ttttgagcgt tagcaccgga cgcagccgcg cgacaggaat aattaaaccg gcctgcacca
    10681 gacattgcac cacgctttca gccgatttat aggcttgtgg cgcttcttca aagatgagtt
    10741 gtttatcgcg acaaattacc cgactgccaa gttcagtacg cgagagttgc gtcgccgtgt
    10801 atttcgctgc cagacgccct ttacactcgg tgcgccccca tttacgccct gccccatgcg
    10861 ccagcgagtg gagcgttttt tcgttcgcaa caggctgtac aatccaggag taatcaccac
    10921 gtgaaccggg aataatcacc agaccgttgt catccggcgt ggcaccttta cgatgcaacc
    10981 agccctgttg atcgccgatt tgacacgcgc taacaaagtt atgcgccaca tccagaaccg
    11041 gacttcccgt ggccttaact tgttgcatta tgcgcaaagc gatcatctgg cgattaatac
    11101 acgcaaacgc cagcgcatcg tcatgttccg caatataccg tagcgcgtcg tcactgcctt
    11161 gaggcaaacc atgatgcgag aacgaggcaa tatgccgctg taaaatagac tgccccaccc
    11221 cccgcgagcc gctatgaacc aacagttgca gatgttgtgc atccagacct gccagtgcaa
    11281 acagttcagc gttgatgatt tgatcaactt gttgcagttc agcgaaatga ttgccgccac
    11341 cgatagagcc aagcgagctg cgccacggat gctgtgcaaa tgctgacggc aggttttcct
    11401 ccagccagct ttcttcagca acgtcatcca gatctgataa tcgcttttca aacttatcgg
    11461 cgttgtattt gcgagcgaga atatctgttt gccatagcgc cataccgcag ccgatatcat
    11521 tgccgaccag tgccggataa aaacgaccaa cagagaagaa cgctgcgcca atcggatagc
    11581 cgcgtccggg atgtaaatct ggcatcccca ctacgcgctg catgttgggt aaatttgccg
    11641 tggtgtgtaa ttgttggatc gctaaactct cgatccacag gtcatcagat gcgatggtaa
    11701 ataccgcatc ggataaggga cgaatataat tgcccatata ccattccgta aatagactcg
    11761 tcatacttca agttgcatgt gttgcgactt gaattattga gagtaacaat taaaaaatca
    11821 ggaaatggca gacgaatcca gggcaagaaa aaaagaatta atcctggaaa ggtctgcaaa
    11881 gagtcattga taaaggattt gtcatgattt acctccttgc agtttgtgga ataagaaaac
    11941 gcgcacatac taggcttgcg ttattttcat tgcaagctgg atttaatgtt gctgtttata
    12001 acgcatataa aaattaataa accactctcc ccgacattca acgcgttgct gcttaactgt
    12061 ctgaaaacca taacgttcaa aaaagggttt tgcggttatg cttgcgtcca ccgtaagttc
    12121 ggattcagac ttaatcaaag gttttaacaa agcgctggca accccacggc gggtgtattc
    12181 agggtcaaca aataacatat cgatatagtg ttcaacgcag gtaataaagc cgaccagttt
    12241 tgcattaatg actgcaaccc gcacttgtga tttcgcgagt ttctccttcc agcgagattc
    12301 gtcaatctgc gcccaggcgg caatttgttg tggtgaataa tgctgactgg cagtcatcgt
    12361 aaccgctcta atgaaaatag cgcatagttg ctgaaaatcg ccaggctgat agtctcttat
    12421 ttgtatgtta ttcataatcc cagcaccagt tgtctttcca tttgcgggtc aagcaacgtc
    12481 acatgcagcc ccaccagtcg cacaccgcgc ccgccgcggc gttcatccca ggttttacgc
    12541 gcggtggcga ttaagtcagc tttattcagc cgcggccaga cgtgctcctg agtggtttgc
    12601 tgaaaatcat caaactttaa tttcactccc tgacgggcga tcagtaaatc aggttttacc
    12661 tttgccagac ggcgttcaag ttccggatac agccgctcga taatcgcttc acattcagac
    12721 cagtgatgaa tatcttccgc catcgtgcgt tccacgccga cggattttcg taaccgttcg
    12781 ctgttaacgt cgcgctcgtc aatcccctga ctacgctccc acaaaatgcg gccaaatttg
    12841 ccaaagcgtt taagcagcat caccagatca cacttttgta catcaccgca ggtccgcagc
    12901 cccatcgctt ccagttttgc cgccgagact ttgccgacgc cggggatttt tgccagcggt
    12961 aaggtttgta aaaatgccgg aacttctgcc ggcgtaatca caaactgacc gttgggttta
    13021 ttcatgtcgg aggcgatttt ggcgagaaac tttactggtg ccacgcccgc agacgctgtc
    13081 agttgcagct cgctgaaaat cgtctggcgg atttcctggg cgatgagggt cgcagaaccg
    13141 tggcaatgga cgctatcggt gacgtcgaga taagcctcat ccagtgacaa cggttcaata
    13201 cgcgaggtgt agcgcgagaa gatttcacgg atatgatttg aggcttcttt gtaggcgtca
    13261 aagcgccccg gaagcaaggt gagatgcggg cataatttga gcgccatccc tgtcggcata
    13321 gcgctacgta cgccaaattt acgcgcggga taattggcgg tgctgatcac cccccgacgt
    13381 tcgcggctgc cgccaatagc aatagggata tcgcgcaggg cgggattgtc gcgcatctcc
    13441 accgctgcga aaaagcagtc catatccaca tgaatgattt tacgcattac tcacctctca
    13501 acactggtaa agtatacagt gatttcaggg tttgagaaat gcgtaaagat tcagcattta
    13561 acgcgttcgc tgcgaaagca cctgctgctt atccagcttt tgcaccagcg gctgcaccac
    13621 tttatcccca tgctgaccaa agtattgata cagcgtatcg gacgcacttt tggtcagcac
    13681 cataatctca atgcgccggt tgcccgcgct ttgcggattt ttggaatcca gcagcatctg
    13741 gtccgccatt gcgcttacct gcatcacttt atcttccggc attccggcct cttccagcac
    13801 ccgacgagcc gaaagcgcgc ggtcacccga aaggttccag ttgttgtaga tattgttttt
    13861 gtaagccatc gcatcggtat gcccggtaat aataattttg ttatcgagcg agtcgaaaac
    13921 tggcgcgagc tccaccagca gcgttttaaa gaacggcata atctgcgcgc tgccgcgttc
    13981 gaacatattg cgattctggt cgtctttaat cagtacgcgt aatccctggg gaacaatctc
    14041 catctccaga ttcgcttcca tatgcgcatc acgggcaatg gtgttgatac tggttgccag
    14101 ctcgccaagc tcggtggcgg atttcttctt cagcagcgcc gttttctcat tcacatcacg
    14161 cgcttttttc tccgtctctt ccggcaccgc cacggtggca ggttttggcg gatgagacgg
    14221 gctgatttta tttaacggcg acagcccgcc accgttaaaa atcgactgcc cgtgcagcgc
    14281 ggcgataatg ctttcacgct cggatttact gacgctgttg acaatccaca gcgtcataaa
    14341 cagcgccatc atcgccaggg taaagtcggc gaacgccacc ttccacgccc cgccatgatg
    14401 cccggcatga ttctttttga tctgccgccg gacgatggtg gtttttgccc cacgctcgcg
    14461 gcggttgccc gtctttctca tgcttattct tgctccagca tcgcattcac ccacgcgtcg
    14521 aggttggcga aggtcggttt ggacgccaga tgcagcagct tacgcccggc atccaccgcc
    14581 agcagcgtcg gtttgccgcc tgcctgcgcc accagcacag tgcgcacgca ttccagcaaa
    14641 gaatgttctg cccgcgcctg ttgttccatg gcgttggcga gcgggtccat caggcagtag
    14701 cagataaaga cgcctaaaaa cgtccccacc agcgctgccg ccactttcag gccgatcagg
    14761 gcaatagagc cgtcaatcga ctgcatggtg atgatgatcc ccagcactgc tgcacaaata
    14821 ccaaatcccg gcatcgcttc ggcggtacgt tgcagcgagc gcgacggcgt gagcagcgac
    14881 tcttccgcag tgtccagttc ctgatcgaga atgccttcca gttcatgggc gtcgatttta
    14941 cccatcgcca tcaggcgaaa gttatcggca ataaaggtca ccagccgctt ctgggttaac
    15001 accagcgggt atttctggaa aatggtgctc tcttccggtt gctcaatatg ttgatccagc
    15061 atgcgcagac caccgttttg caccatttcg agcaactcat acaggcacat taatagctga
    15121 cgctgaaact ccggcccgag ctgtttttta ctgatcacac ctttaatttg atgggcaatc
    15181 tctttcagca catgtttcgg gttaccaata atcatcgccc cgaaaccggc acccaaaata
    15241 atgatcatct ccgccggttg ccagatagcc actaattgcc cgcccgcttc aaaataaccg
    15301 ccaaatacgc agccgagcac caccagtaaa cctaatattt tttgcatgat ttcttcactt
    15361 taattcggat agacaaaatc acgaattttc tgcgcgatct gtttattcag ttgacaaatt
    15421 cgggcctcgg ttagccctaa taccagcgca atctctttga gattcatctc atgctggtaa
    15481 tacatggaga ggatcagttg ctctttttcg cttaattgtg aaagcgcctg ttgcagcatt
    15541 tgctgagtga caaactgatc ttccagctcc cggccaacaa gcggaatgcc tggcccttcg
    15601 ccgttcagca gttcgtccag gctggcgagc gtttccgccc cttccagttg ctgatattcg
    15661 agataatctt catgtgagat cccttccacg gccagttctg accactccgg ttcgtgcccc
    15721 agttttttgc gcgtttcgcg aatcagatct ttcatctgat gatatttctg tcgcagctgg
    15781 cgcggtcgcc agtcgagagc gcgcagctca tccaggatcg cgccgcgaat acggtgcacg
    15841 gcatagcccg caaaaccttc gtccggcagc ccgtagcggc ggatggcatt aagcagcccc
    15901 attaacgccg tttgttccat atcctgacga tcaataatgc aattacactg cggggcaagt
    15961 tgccgcacca ctttacgcac caacggtaaa taggcctgca agtagtggct ctcctgctgc
    16021 ggggttaata ccggcgcggc agcaaattct tctgactcga atacatcctg tagcatgttt
    16081 atatccgcct ggtgattact ggaagaccac ttttttcacc agcaaatcct tatacggtgc
    16141 gctcattttg cgacgctgaa gatctttctt taacgcgtca gaaatgagtt tgcggatggc
    16201 agatattttc atgccgcgca aatcttcaaa tttcatttcc gtcaggcatt caaccgcaat
    16261 actttgatac agcggctcct gatgttttat tttttccgct tctttgtcac tggcgaccac
    16321 catcactaat tccgccgaca aataatggtc cgcgccgttg ttgtcatgca gcgtgacaat
    16381 ggtttccggc aacgtcacaa acacgctttt gctttcatcc agggtttcca ccgtttccgt
    16441 ctgcgcggcg gcagtcggtt ttccttttcc ggcataatga tgccagacac cccatccagc
    16501 tccggccccc accaccaacg ccagaacggc gctgaccacg cccgccacca cgattttctt
    16561 catattgcct ttacactttg atcaaaacgg attcgttctg ttgaaattga ggcgcatcat
    16621 caagctgtaa ggcggcactc acctcttcct gctgatggcc cgactgttgt tgttgctgcg
    16681 actgcccttc ggaggaaacc tgtacgttca cctccataaa attttgcgcc gtcagatgct
    16741 ggcggagatc gtcgctaaac tgctgtaacg cgcggcaaac ttcactttgg ttcgcgccga
    16801 tgtgcaccat cagtttgccg ttgtcgagtt gtacggcgat ctcgagcttg ccaagcgacg
    16861 gtggatcgag acggatggtg gagatctgct gttgtttgtt gagctgaaag tggatctgat
    16921 ctttcaaaag cgtcgtcagt ttttcgccca gttcgtccat cgaaagcgtg gctggtttgg
    16981 cgacaatctc ctgatgatcg ctgagcagct ccggcgtctt aacctgcaac accgcacgcg
    17041 caataaccgg ttcactgtcc ggcacttttt ccgttttacg ctcgacctgc gggcgcgcgg
    17101 tcacacgggt tagttcagat ttcgggcgag ctggctgtgt tgagacgcgc ggctgcgtcg
    17161 gcgtaatggc atgtaaatct tcactagcgt aagtcgccag tctggcctgc tgttcgggct
    17221 tcgcctgcgg caactgactg atcagttcct gcaactgcgg cggcaacggc gtgagttcct
    17281 gctgttgcgt ctggcgttgc ggcgcgtttt gcaccaccgc tttcgtcaat tgctggataa
    17341 ccggcgatgt tgcgacgttt cgcggctgcg gcgtgtcctg atgcggcgcg gcaggttgcg
    17401 gaagtaacag cgccattagc gcctgcatcg ccaccggatc gttctcctgc gggcgcgtcg
    17461 cctgttgctg actggttttt gcgctatgaa cgcgctccgc caccgccgcg accgccattt
    17521 tcggcagggt aaacgcgggc gcgttttcac cgctcaccgg cggtaaaata tccgccttca
    17581 acgaagcggt ttccgcaagc gttccgagcg tggcaagcaa tgtcggattc atgacttctc
    17641 cgaaagtaaa ttaacaattg agtaggccag tacgccttcc tgatgccgct gatgctgttc
    17701 catacgcgct ttcaacactt ctgcgcgctg ctcgcgccgt ttaatcaggg cgttaaacgc
    17761 gtcatacagt cgcgctttca ctgcatccaa ctgcgccgca tcggtttccg ccgccgcgta
    17821 atcgtgaaac tggcgtatca actgctgatg cagtgccgga aggcgtcgcc agcgttgctt
    17881 gtcgagggct tcattcatcg cctcgaccag tgcgaaaagt ttcctgcgtt gtttttccat
    17941 cgtcttaacc cagcttgccc gaaagccctt cccagccttc gcgcaggttg cttaagatca
    18001 gcatcacttc atcgattttc tctgccgaca gttcgccgct ggcctcatac aatcggtaaa
    18061 cgcagtggtc atagagtcgc gaaaggttga ccaccagctc gccgccagtt tcaaactcca
    18121 gcgaactggt gagcgcattg agaatgtcga tgcatttatt gatgctctgg gctttcttct
    18181 cgaaacgccg cccttcaata tggcttttgg cgcgttccag ttcgtccatc aggccagaaa
    18241 acagcaccag caccagttcc agaggcgatg ccgccgcagt acgcgccgcg agatcgatct
    18301 ctttatattg cgaatagccg tcctgtactt ggtacatctt tagggatatt ccttctgagt
    18361 tacgcgaatg ccgccatgct ggctttcatg gtgtacacct cgaccagcgt gttggtgtac
    18421 tcctcgagat aacggtcgta gttggcgtta taggtatcag taagctgatc gccttcgtcc
    18481 tgaattttgc tcatctgatc gtcaatgttc tgctggcgca gggtgatgat gccgttgctg
    18541 gagtccgtgt aggtgttaat aaggtcgtcc atctgcgcca ccatgctgtt gtcgccaacg
    18601 aaaatagagg tcagaccgtc aggatttttc gccatttcgt cgttgaactg gtcggagtca
    18661 atctgtaagt ggccgtgaga atcgagggtg atgccatagt caacaatcga cacgccgttg
    18721 tagctggcat gggcgatgtc atcgagctgg ttcgccagtg aactgagtcc ggcgtcgcct
    18781 gcaaataccc cggcgctggt actgtcatca ccgtgggtgg ttagcgaatc aaccgtatca
    18841 atcaaggtgt tataggcatc gacaaaggtc tgtactttct cctggctggc gctggaatct
    18901 tcgctgatgt taaaggtagt gagatcgctg tcagaatcgc tgacttcggt gaaagtcatg
    18961 gtgacaccgg gaatcacatc atcaaacgta ttgctgctgt tggtgatctc aggccccgtg
    19021 gcgctgccaa gatgaataat cgcgtcctga gcggaggaaa catccgtggc aacgggcgcg
    19081 ctggtgctat cgttgctggc atcatgcccc gttacgctca ccgaaaacgc gctctgcgcc
    19141 ccggtgctat ccgaggtgag cataatcgtt gtggtgccgt cggtttttac cagcgccgcc
    19201 gacacgcccg gattatcatc ggagtcgtta atcgcattca ccagttcact ggcgtcgata
    19261 aaaccgtcgc cgtcaccatt ctgatctgcc gccgacagat cgatatccat ggtgctgtcg
    19321 cccatcgtca gttcaaaagt gccggtggcg gaaaaggcgt cgtcgcccat actgaacgtg
    19381 gtttgctgcc cctgtgccag ttgctcaacg aaaaatgagt agcttcccgc ctgcgcctgg
    19441 gaattggcgg aaacgctcgc cgagtcatta ttgctggtgg cggcaaacgt caccgggccg
    19501 tcggtatcgc tgttcagcgc gtcaacggcg ctctgaaaat cgctcaacgc tgaactgagc
    19561 gaatccaggc cgctgctttc agcatccagc tctgtttgct tctcctgcaa gtttgccgcc
    19621 tgagtggcga catccgcata cgcgatttct tgcgcaatgg ttcttgggtt gatcattgat
    19681 gactccggat aacgatgtca ggttattggc aaaaggcgtg ccaatatttt ttatgtgaat
    19741 aatcaataga ttaaaaaaca ggcggaaatg gcgtttccgg tctggcggaa aaaagcctgc
    19801 cgcagaaagc acaacaccgc ccgcaggcgg tgttgttcgt agtccgaata aggcgtttac
    19861 gccgcatccg gcaagtccgc cattactgca gcaggctgga aaccatgctg gacatgctgt
    19921 tggactgttt cagcatggtg atgccagtct gcatcagcac ctgctgtttg gtcatattgg
    19981 acgcttccgt cgcgtagtcg gtatccataa tgttaccgat agcgacttcg gtgttgtcct
    20041 gcatactggt caggttgttg gcggtgtcgt tcagacggtt gatagacgca cccagcttgg
    20101 actggatctg cgaaacgtca tccatcgcgg tagagatgct ggtgatcatc tggttagccg
    20161 agccagacgc ggtcagctca gtaccgccag aaacacctgc agtacccgtg gtgtctgcct
    20221 gatccgcgct aaaggagttg ctgatagcag aaagatcggt caccagcgtg ttcagttggc
    20281 tggagatgtt cacggtcatg gtgtcggaag attccgcgcc cacctggaaa gttaccgcgc
    20341 tctggaacag accgtcagta ccggtgttgc tggtaccgga aacaccgaac aggttggtgc
    20401 cgccgtaagt ggtgttttgc agcatgtcag acatctgctg acccagttcg tcgtattcgt
    20461 cctgcatcgc ctgcagatcg tcgtcgctgt aggtgccgtt cgctgcctga gttgacaggt
    20521 ctttcatacg acccagcaca tcagacattt catcgaacat actatcggcg gtttgcagca
    20581 tcgcggttgc gtcattgatg ttgctcagcg ccacgcccat accgctggac tgtgctgtca
    20641 gacggttagc aatttgtttg cctgcggcgt catcggcaga agagttaatc cggttacctg
    20701 tcgccaggcg ttccatagat gtagacaaag aagagctgct tttgctgatt gcattaacgg
    20761 cggccattga ggcgttattg gtgttaatag ataacatgat actgctcctt gggattaatc
    20821 gttttcgttt cgataccctg taaaaacgac acctaaggag caaacttaaa accgccgtta
    20881 aaaaaatttc actgcgattt atctgatgcc acttttagct aaaaaaagca gagtttaaat
    20941 tcagataaaa agattattag caatatctgc cacatggcca ggtatcaaaa tatgtcttta
    21001 tcaaacgctt gatatagtta agattaccat caattaacgc gttttcttct caatgtactg
    21061 atgccaaaat ttacattgcg ttgcaaatag tacacatttg gctattgtac cagtcctgtc
    21121 atttcgttac atttccatgc catatagtga ttaagaataa tcctattacc ataaaatatt
    21181 agttatcacg cagaaaaatc atcttctcag ggacgtataa gactgtgctt tatgcaaagc
    21241 actatcagga aacatttatc ccttgctaag gtaaactcaa ctatgagtat tattatcaac
    21301 aactggcgga tggacccgtc gcttaatgcc ttaatacatt gcgaaacggg tgaaacacgt
    21361 cgtctcggcg aataccattt tattttgctg gaaaaattag caaaaaatgc cgatgtggtt
    21421 ttatcgcgat cttatttatg tgccgaagta tggaagaacc gcattgtcgg cggaaacagc
    21481 ctgccgacgg cgatccatgc gttgcgcgtt gctatcgatg atgatggcaa acaacagaac
    21541 attattaaaa caattccgaa gaaaggttac ttatgcaata aagagtatgt gtctttgccg
    21601 gactcatcgc ctgcaaaaaa actgattatt accgaccaga tacaggaaac agtaccagaa
    21661 gagatatctt ctacaacacc gcctgtgcct gttagaaaaa aacataaagt aataatgggg
    21721 ctagcgttag ccgcggctgt tatatttatt ggctcgaccg tggggtattc acacttgaaa
    21781 agtacacctg atgccccgca actggtaaaa gagtcaatta acagtccaag aataaaaata
    21841 tttcacctta gttctggaaa agaaaataat tccgtaccgt tactgtcaca aaccctcgca
    21901 ccaggaaaag acaaactcga taatttattg tcagcgcata atatgacaat gacaacctat
    21961 tataaatatg ttcgtaaccg actggaaagt gatattgttc tgcgcaacca gtgcaatggt
    22021 agctggcaat taacttttaa tgtggaaggc tggcagaaca gcgatattaa tagcgcgatg
    22081 tatcagaatt tagagaagtt gttaaatact ttgcagaaat gctgatgagt gccaatatat
    22141 gccggatgcg acgctaaacg cgtcttatcc ggctgataat taccccaata aattcctcac
    22201 cgtctggctg gaaattcgcg cctgaccatt caccgcctgc aataaaacct gatagttatg
    22261 attcgcgcta tccagcacgc cgccgaggtt ttccgacgcc tgcaccgcgt tttccgtttg
    22321 cgggaagcgc gacatgccgt cgatcaactg ccgcgccttt tcctgctgta ccgccatctg
    22381 cgcacgctgc tgattaatgc tttctaacgt ctgagaaatt cccgcaccgc cctgttgcag
    22441 gctttgagct aaacggtctg cctcagacgg ctcggcaaaa gttttcagcg gcataaacgc
    22501 cgacttatcg ccatcggtgc gcacgcttaa ggtacgttcg atatggggcc actgtttttc
    22561 ggtggtggag aaactgatgc cgtccgcttg ctgatgtagc tgcacgccca cgcggcgcag
    22621 ggcgttggtc agccgcgtct gatactgcgc ggcgctgtca tcctcagaca gcatcaccgc
    22681 cgaaagctga gtctgccgcc cgtcagagac gctgaacatg cgcgttcccg gcgtgggatt
    22741 gtgtaccaga ttcgccagat ccggcgagtg aaaggtcacc cgcgcttcgc cctgtaacac
    22801 cggctgcaac tggcggtcca ccgcaccgcc ggaaagcgcg gtgcgtttgt ccagcatctg
    22861 catcagcgcc gtattctgcg cctgcccgcc tttacgctgg ctgtgacgat agtcgagcaa
    22921 ctgctgctcc agttgcccga gataatgatc cgcctgctgc aacgtggtga gctggtcgtt
    22981 aagctgcacg ctgtaacgct gcgggcgagt ggtaatcagc ggcgaggccg gatattcact
    23041 cgccgtcgcc gggagttttt gccgcaccgc ctgagtgggc gcaacggttg acgacgatac
    23101 cgcagcgcta tgcgcgccgc cggtggcgag tgatgaagat gtaagtccga cctgcatact
    23161 gttcctgaaa gagttagaga acgctgaaca aattcaactc actcactttc gagtaagtct
    23221 tgagggtaac ttccattgcc acttccagtc cggtaaaggt gatcgacgcc gggccgtaat
    23281 cgagatcctg taatgaacca atcagctcat cattagaggt ggagatatcg gtctgcgcat
    23341 cgctcagcat cgacatggta ttttgcgtct cgccgagtga cgcaatcgag gcgttgaggt
    23401 cgtccgaggc ggtgtccacc acatcaacgg cattctgaat atcgctctgc acctgcggat
    23461 ctgccgggtc gacgtccggg ttttgcaact cctgggatag ggaattcagg gtgttgagca
    23521 cgtctaaatt gctgccaaag aaatcactcg ccgccacgtt ggtgtccact tccacgccgt
    23581 tcgacaccgt ggtctggcgg tggtcgctgt taccctgata gctgtagctg tcggtgacgc
    23641 catcgccgtc atcgtccact gccacaatcg gcggctgatc gttgatggtg ccgccaaaca
    23701 cgtagtgccc gtcttcgtcc tgataattga gcgccgccac cattgattcg gtgagcgact
    23761 caatatcctg cccgaggctt gcgagtgaat cggcggtatt ggtgccattc gccgcttcca
    23821 gcaggtcgtc gcgcaccgcc agcaggctgt tgttgacgcc atcaaggatc gactcctgct
    23881 ggcttaagcc cgccgacgcg gagtcgatat tgctctgata ctgctcaatc gccgactgct
    23941 ggcggtttaa ctgggtgatt cgcgtggcgg cgatcgggtc atccgacggc tgcaaaatct
    24001 gcttgccggt tgccatcagc tcgacaacat gcgccagatc gccagagaga ttattaaaac
    24061 tttgcgtcat cgagacgtag gtttgttggg tagttactcg cattttcgcg ctcccgctta
    24121 gctgcacatt tccagcaccg aatcgaaaat ctcggccccg gcggaaatga ctttcagatt
    24181 ggcttcataa atttgttgat aggtgatcag gttcaccgcc tcttcgtcca tgctgacgcc
    24241 gctgacgctg ctctgctggt tttgcgctgc ggaatagaca ttggacgcgg catcgacttc
    24301 cgtctggttt tgctgactgt aaatgccgat attgctgatg attgacgagc acgcctgccc
    24361 gaccgtcacg ctgccgaggt tggctatttc cagcggttcg gtggagatat tgatcagcgc
    24421 ctgcaggttg tcgctgttgc cgctttcgtc cggtgaactg gaaaacgcca gctcatcggc
    24481 ggtgatattc gggttaacgg tcagcgggcc atcggcattg ctggcgtcat aaataaacag
    24541 cggctcgccg gggttaccgt tgagatcgta gccctgcgcc agctggttgt tgaccgcatc
    24601 ggcgaactgc gacgccatgc tgttgatggt gtcggtcagc ggcgtcagca catcgttttg
    24661 ataatcaaac agtgcgccta atgaaccgcc ggtgtcggtg gtcatcgtcg aggtggtgcc
    24721 agcgaaggtc agcgacatgg tcgacgtgcc atcggcgttg gtttccagcg cgatggtcga
    24781 actttgctgc ccactcacca gcggctgacc gtttttcagg gtaacgttgt agttgccctg
    24841 gtcgtcgata ttgacctgca catccatcat cccgctcagt tcttccacca tctggtcgcg
    24901 ggcgtcgtac agcgcggagg cgttatcgcc attggcttca gcctgagcga tttgctggtt
    24961 atagctggcg atgccgctgg tgagcgtatt gatttgcgac accatcgcct gctgctggct
    25021 gatgatttcc gtgctttgcg agtcgatgta atccagcgtg ttatcaatac gcaacgacag
    25081 cgccccggct tcactgatca cctgctcgcg cagggcggaa tcatcggggc tggtggttgc
    25141 ttcgttaagg gcggcgaaga agttatcgaa gccgccgctc aggctgctgt tatcgtcgct
    25201 cagtaccgct tccagttggc tgagatcccc ctgctgggtg ctgtaatagc cgtaatcgct
    25261 ggcggcatac cacacctgat tcacctgata ctggttggag acgcggcgaa tgctgtcgac
    25321 ctgtacgccg ttaccggcgc tgttcggcga accgccgctg gcaccgatgg tgctgatctc
    25381 cgccacctga cgggtgtagc ccgtggtcat ggcgttggcg gtgttttgcg ccgtgacgtt
    25441 aagctccacc tgcgcggttg atgcaccgct gtagccgatg ttaatcatgt ccatgttgga
    25501 tccttaccgg taaatcgtgc cggttaaaag cggcgtaaag ttggggattc ttaagttccg
    25561 taaactgggt gccgaacgcc ggatgcggcg tggacgcctt atccggccta catacgggcg
    25621 actcgtaggc ctgataagac gcgacagcgt cgcatcgtgc accgaacgcc gaagctcatt
    25681 tatgcttcgg cgacaactgc ttcacaatca tcgccgcaat gccaatcccg tgctgctgcg
    25741 ccagggtgtt acacaactca tcgtcataaa acccctgcat catgcgcacc gaatcgctgt
    25801 taaacgggtt atcttttgaa tcaaacaact cgttggtttt ccgcatctct ttcagcatgt
    25861 tgcgtaaaaa aatcgcctca aactgttcgg cggcctgctt gatatcattg gcctgaacct
    25921 tcggccccat taacgcgtcg ctgccgtcaa tcccgccgct ggcgttcact ttcatcagat
    25981 cacctccagg tcggcatcca gcgcgcccgc ctcatgcagc gcctgcaaaa tcgacataat
    26041 gtcgtccggc gtcgcgccta aactgttcag cgcattgacg agcgttttca ggctgctcga
    26101 ctccggcaaa ctcaccacgc ccggacgggc atgattcacc gcgatattgc tctgcggcgt
    26161 aaccgccgtg cgcccacccg caaaggcatt cggctggctg acgttgctgg tttcgttaat
    26221 cgaaacggtc aggctgccgt gcgacaccgc cgccgcgtgt agcgcaacgc catcgcccat
    26281 taccaccgtg ccggtacgcg agttaaacac cacgcgagcg cggattttct ccgcctgcac
    26341 ctgcacatcg tccagttgcg acataaatgc cacccgcgcg ccggggctgg tgggcgcgcg
    26401 cacggtaacg ttggtggaac tctgcgcggt ggcaataccg ccaaacgagg cgttgatcgc
    26461 cgccgcaatg ttgttggcgt ctttaaacga cgggcgtttc aggttgaggg tgatggtatc
    26521 gcccatctga aaatcgctgg ggatctcgcg ctccaccgtt gcgccgttag gaattaaccc
    26581 ggcggttggc gtattaaccg ttacgctgga accgctggcc ccggtggcgt tcatcccgcc
    26641 caccaccacg ctgccctgtg ccagggcgta aacttcccca tcagcgccgt gcagttgggt
    26701 cagcagcaaa gtgccgccgc gaatactttt ggcatcgcca atcgacgaaa cggtgacatc
    26761 aatggtctgc ccgcgcgagt acatcggcgg cagcgtggcg ctcaccgcca ccgcggcgac
    26821 gtttttcacc ttcgggtcga ttttattcgg cagttgcacg ccaaactggc gcagcatatt
    26881 ggtgatggtc tggttggtga atttgacctg gtttttatca ccggtgccgt cgaggccgac
    26941 caccaggcta tagcccacca gttggttttc gcgcacgccc tgtacgttga ccagcgactc
    27001 cagcgattgc gccagcgcca caccaggcag cgccaggctc acggcgataa ctgcggcctt
    27061 tatccagttt tgcattgcac tctccggcat taaatcggga acagaggatg gttaaacagc
    27121 cgcgtcagcc agcctgcggc gttggcgtcg cttaacgcgc cgcgtccggc ataggagatg
    27181 caggcatcgg cgatccgctg ggaggaaacc gagttgtcgt tctggatgtc gtcggcgcgc
    27241 accaggccgc ttaagcggat gtactcatcg ccctggttga gggtgagcca tttctcgccg
    27301 cgaatttcca ggatgccgtt gggctgcacc gcatggacgg aaacggtgat ctccccgcgc
    27361 aggctgttct gctgctggga agtggcgctg ccgtcaaagt cgcggttggc gtcgatagag
    27421 ccggaaagct tatctttagt gtgactaaaa atggtcggtg cgccgatatc gacggtgttg
    27481 cttttgccga agttggtttt cgcctgtttg ctcgactggg tggattcttc gaggatcacc
    27541 gtcaggatat cgcccacgcg ataagcccgc cgatcggcgg tcagcgacca gttgtagcca
    27601 gtttcaaata caccgccagc acgcccgtct gccggaggcg gtgcttcggt tttcggtggc
    27661 gcaaagtatg cgtcgtcttt tttgaccaga atggcctgcg attcacaccc gctaagcagg
    27721 ggaagcagcg ccaccagcca gagataattt ttcacgttct gcctgtttaa atacagtgag
    27781 ttatgcgctt ttgtaggtcg gataagacgc gtcagcgtcg catccgacat ttcggcctta
    27841 cagcgtctga ctgatgtact gcaacatatc gtccgcagcc gacaccattt tggcgttcat
    27901 ctcgtaagcc cgttgcactg taatcatcgc caccatttcg ttgacgatat ctacgttcga
    27961 gccttcgagg gcattgtcct gcaaagtgcc aagcccatct tcgcccggca caccttcggt
    28021 cggctgaccg ctggcggcgg tttccagata caggttgtca ccctcagcgg aaagcccagc
    28081 cgggttagcg aagttaacca gcgtcagttg ccctaactcg gtcacgtcgc
//
LOCUS       DAZGLJ010000040        27403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000040 DAZGLJ010000000
VERSION     DAZGLJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.040"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            15..89
                     /gene="tnaC"
                     /locus_tag="ACHK49_001545"
     CDS             15..89
                     /gene="tnaC"
                     /locus_tag="ACHK49_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO9262214.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            310..1725
                     /gene="tnaA"
                     /locus_tag="ACHK49_001546"
     CDS             310..1725
                     /gene="tnaA"
                     /locus_tag="ACHK49_001546"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO9262215.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMSLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1816..3063
                     /gene="tnaB"
                     /locus_tag="ACHK49_001547"
     CDS             1816..3063
                     /gene="tnaB"
                     /locus_tag="ACHK49_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO9262216.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3195..4370
                     /gene="mdtL"
                     /locus_tag="ACHK49_001548"
     CDS             3195..4370
                     /gene="mdtL"
                     /locus_tag="ACHK49_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO9262217.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4345..5304
                     /gene="yidZ"
                     /locus_tag="ACHK49_001549"
     CDS             4345..5304
                     /gene="yidZ"
                     /locus_tag="ACHK49_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO9262218.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5461..6210
                     /gene="yieE"
                     /locus_tag="ACHK49_001550"
     CDS             5461..6210
                     /gene="yieE"
                     /locus_tag="ACHK49_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO9262219.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            6232..6798
                     /gene="yieF"
                     /locus_tag="ACHK49_001551"
                     /gene_synonym="chrR"
     CDS             6232..6798
                     /gene="yieF"
                     /locus_tag="ACHK49_001551"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO9262220.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6852..8189)
                     /gene="adeP"
                     /locus_tag="ACHK49_001552"
     CDS             complement(6852..8189)
                     /gene="adeP"
                     /locus_tag="ACHK49_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO9262221.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8356..9021
                     /gene="yieH"
                     /locus_tag="ACHK49_001553"
     CDS             8356..9021
                     /gene="yieH"
                     /locus_tag="ACHK49_001553"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO9262222.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9088..9561
                     /locus_tag="ACHK49_001554"
     CDS             9088..9561
                     /locus_tag="ACHK49_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9262223.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELYENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9610..10197
                     /gene="cbrC"
                     /locus_tag="ACHK49_001555"
     CDS             9610..10197
                     /gene="cbrC"
                     /locus_tag="ACHK49_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFO9262224.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10259..10981)
                     /gene="yieK"
                     /locus_tag="ACHK49_001556"
     CDS             complement(10259..10981)
                     /gene="yieK"
                     /locus_tag="ACHK49_001556"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO9262225.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALD"
     gene            complement(10996..12165)
                     /gene="yieL"
                     /locus_tag="ACHK49_001557"
     CDS             complement(10996..12165)
                     /gene="yieL"
                     /locus_tag="ACHK49_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO9262226.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(12192..13808)
                     /gene="bglH"
                     /locus_tag="ACHK49_001558"
     CDS             complement(12192..13808)
                     /gene="bglH"
                     /locus_tag="ACHK49_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO9262227.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13894..15288)
                     /gene="bglB"
                     /locus_tag="ACHK49_001559"
     CDS             complement(13894..15288)
                     /gene="bglB"
                     /locus_tag="ACHK49_001559"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO9262228.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDSVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15307..17184)
                     /gene="bglF"
                     /locus_tag="ACHK49_001560"
     CDS             complement(15307..17184)
                     /gene="bglF"
                     /locus_tag="ACHK49_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO9262229.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(17318..18154)
                     /gene="bglG"
                     /locus_tag="ACHK49_001561"
     CDS             complement(17318..18154)
                     /gene="bglG"
                     /locus_tag="ACHK49_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO9262230.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(18440..19165)
                     /gene="phoU"
                     /locus_tag="ACHK49_001562"
     CDS             complement(18440..19165)
                     /gene="phoU"
                     /locus_tag="ACHK49_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO9262231.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(19180..19953)
                     /gene="pstB"
                     /locus_tag="ACHK49_001563"
     CDS             complement(19180..19953)
                     /gene="pstB"
                     /locus_tag="ACHK49_001563"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO9262232.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(20044..20934)
                     /gene="pstA"
                     /locus_tag="ACHK49_001564"
     CDS             complement(20044..20934)
                     /gene="pstA"
                     /locus_tag="ACHK49_001564"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO9262233.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(20934..21893)
                     /gene="pstC"
                     /locus_tag="ACHK49_001565"
     CDS             complement(20934..21893)
                     /gene="pstC"
                     /locus_tag="ACHK49_001565"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO9262234.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(21980..23020)
                     /gene="pstS"
                     /locus_tag="ACHK49_001566"
     CDS             complement(21980..23020)
                     /gene="pstS"
                     /locus_tag="ACHK49_001566"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO9262235.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(23268..24341)
                     /gene="lpfD-O113"
                     /locus_tag="ACHK49_001567"
     CDS             complement(23268..24341)
                     /gene="lpfD-O113"
                     /locus_tag="ACHK49_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFO9262236.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPSPGQFTATATITVEIVR"
     gene            complement(24352..26874)
                     /gene="lpfC-O113"
                     /locus_tag="ACHK49_001568"
     CDS             complement(24352..26874)
                     /gene="lpfC-O113"
                     /locus_tag="ACHK49_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFO9262237.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVST
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(26899..>27403)
                     /gene="lpfB-O113"
                     /locus_tag="ACHK49_001569"
     CDS             complement(26899..>27403)
                     /gene="lpfB-O113"
                     /locus_tag="ACHK49_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFO9262238.1"
                     /translation="FNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRES
                     VFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNL
                     IAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFINDYGAVS
                     DPIKMTL"
BASE COUNT         6964 a         6883 c         6424 g         7132 t
ORIGIN      
        1 aggataagtg cattatgaat atcttacata tatgtgtgac ctcaaaatgg ttcaatatcg
       61 acaataagat tgtcgatcac cgcccttaat ttacccttct gtagccatca ccagagccaa
      121 accgattaga ttcaatgtga acgattagtt tgctatatct taattttgcc ttttgcaaag
      181 gccatctctc gtttatttac ttattttagt aaatgatggt gcttgtatat atacatggcg
      241 aattaatgga tattgcagat gtaatattca cagggatcac tgtaattaaa ataaatgaag
      301 gattatgtaa tggaaaactt taaacatctc cctgaaccgt tccgcattcg tgttattgag
      361 ccagtaaaac gtaccactcg cgcttatcgt gaagaggcaa ttattaaatc cggtatgaac
      421 ccattcctgc tggatagcga agatgtgttt atcgatttac tgaccgacag cggcaccggg
      481 gcggtgacgc agagtatgca ggccgcgatg atgcgcggcg acgaagccta cagcggcagc
      541 cgcagctact atgcgttagc cgagtcagtg aaaaatatct ttggttatca atacaccatt
      601 ccgactcacc agggccgtgg cgcagagcaa atttatattc cggtactgat taaaaaacgc
      661 gagcaggaaa aaggcctgga tcgcagcaaa atggtggcat tctctaacta tttctttgat
      721 accacgcagg gccatagcca gattaacggc tgtaccgtgc gtaacgtcta tatcaaagaa
      781 gccttcgata ctggcgtgcg ttacgacttt aaaggtaact ttgaccttga gggattagaa
      841 cgcggtattg aagaagttgg cccgaataac gtgccgtata tcgttgcaac catcaccagt
      901 aactctgcag gtggtcagcc ggtttcactg gcaaacttaa aagcgatgta cagcatcgcg
      961 aagaaatacg atattccggt ggtaatggac tccgcacgct ttgctgaaaa cgcctatttc
     1021 atcaagcagc gtgaagcaga atacaaagac tggaccatcg agcagatcac ccgcgaaacc
     1081 tacaaatatg ccgatatgct ggcgatgtcc gccaagaaag atgcgatggt accgatgggc
     1141 ggcttgctgt gcatgaaaga cgacagcttc tttgatgtgt acaccgagtg cagaaccctt
     1201 tgcgtggtgc aggaagggtt cccgacatat ggcggcctgg aaggcggcgc gatggagcgt
     1261 ctggcggtag gtctgtatga cggcatgagt ctcgactggc tggcttatcg tatcgcgcag
     1321 gtacagtatc tggtcgatgg tctggaagag attggcgttg tctgccagca ggcgggcggt
     1381 cacgcggcat tcgttgatgc cggtaaactg ctgccgcata tcccggcaga ccagttcccg
     1441 gcacaggcgc tggcgtgcga gctgtataaa gtcgccggta tccgtgcggt agaaattggc
     1501 tctttcctgt taggccgcga tccgaaaacc ggtaaacaac tgccatgccc ggctgaactg
     1561 ctgcgtttaa ccattccgcg cgcaacatat actcaaacac atatggactt cattattgaa
     1621 gcctttaaac atgtgaaaga gaacgcggcg aatattaaag gattaacctt tacctacgaa
     1681 ccaaaagtat tgcgtcactt caccgcaaaa ctgaaagaag tttaattaat actacagagt
     1741 ggctataagg atgttagcca ctctcttacc ctacatcctc aataacaaaa atagccttcc
     1801 tctaaaggtg gcatcatgac tgatcaagct gaaaaaaagc actctgcatt ttggggtgtt
     1861 atggttatag caggtacagt aattggtgga ggtatgtttg ctttacctgt tgatcttgcc
     1921 ggtgcctggt ttttctgggg tgcctttatc cttatcattg cctggttttc aatgcttcat
     1981 tccgggttat tgttattaga agcaaattta aattatcctg tcggctccag ttttaacacc
     2041 atcaccaaag atttaatcgg taacacctgg aacattatca gcggtattac cgttgccttc
     2101 gttctctata tcctcactta tgcctatatc tctgctaatg gtgcgatcat tagtgaaacg
     2161 atatcaatga atttgggcta tcacgctaat ccacgtattg tcgggatctg cacagccatt
     2221 ttcgttgcca gcgtattgtg gataagttcg ttagccgcca gtcgcattac ctcattgttc
     2281 ctcgggctga agattatctc ctttgtgatc gtgtttggtt ctttcttctt ccaggtcgat
     2341 tactccatcc tgcgcgatgc caccagcacc actgcgggaa cgtcttactt cccgtatatc
     2401 tttatggctt tgccggtgtg tctggcgtca tttggtttcc acggcaatat tcccagcctg
     2461 attatttgct atggaaaacg caaagataag ttaatcaaaa gcgtggtatt tggttcgctg
     2521 ctggcgctgg tgatttatct cttctggctc tattgcacga tggggaatat tccgcgcgaa
     2581 agctttaagg cgataatctc ctcaggcggc aacgttgatt cgctggtgaa atcgttcctc
     2641 ggcaccaaac agcacggcat tatcgagttt tgcctgctgg tgttctctaa cttagctgtt
     2701 gccagttcgt tctttggtgt cacgctgggg ttgttcgatt atctggcgga cctgtttaag
     2761 attgataact cccacggcgg gcgtttcaaa accgtgctgt taaccttcct gccacctgcg
     2821 ttgttgtatc tgatcttccc gaacggcttt atttacggga tcggcggtgc cgggctgtgc
     2881 gccaccatct gggcggtcat tattcccgca gtgcttgcaa tcaaagctcg caagaagttt
     2941 cccaatcaga tgttcacggt ctggggcggc aatcttattc cggcgattgt cattctcttt
     3001 ggtataaccg tgattttgtg ctggttcggc aacgtcttta acgtgttacc taaatttggc
     3061 taaatccttc aagaagccag ccattcgctg gcttcttgcc tctcaggaaa tcacttatgt
     3121 ccaaatggca actcgcctga ttctccttca ccacgtatgc tttgcgtcac cttactatca
     3181 ggacgcttta gcccatgtcc cgctttttaa tttgtagttt tgccctggtt ttactttatc
     3241 ccgccgggat tgatatgtac ctcgttggtt taccgcgcat cgccgccgat ctcaatgcca
     3301 gcgaagcgca gttgcatatt gcgttctccg tttatctggc gggcatggca gctgcgatgt
     3361 tatttgccgg taaagtggcc gatcgttcag ggagaaagcc tgtcgccatc cccggcgcgg
     3421 cgctatttat tatcgcttcg gtgttctgtt cactggctga aaccagcacg ttatttcttg
     3481 cagggcgctt tctacagggg ttgggcgcag gctgttgtta cgtggtggcg ttcgccattt
     3541 tgcgcgacac gctggatgat cgacgtcggg ctaaagtgct gtcgttactc aacggtatta
     3601 cctgcatcat tccggtgtta gcgccggtgc tcggacacct gattatgctt aaattccctt
     3661 ggcagagtct gttctggacg atggcaatga tgggcatcgc ggtactgatg ttatctttgt
     3721 ttattttaaa agaaacgcgc ccagcgtccc ccgctgcttc ggacaaacca cgagaaaata
     3781 gcgagtcgct gcttaatcgg tttttcctca gccgtgttgt tatcaccacc ctcagcgttt
     3841 cggtgatcct cactttcgtc aatacatcgc cggtattgct gatggaaatc atgggttttg
     3901 agcgcggaga atacgccacc attatggcgt tgaccgctgg cgtcagcatg accgtttcat
     3961 tctccacgcc atttgcgctg ggaattttta agccacgtac gttgatgatc acctcgcagg
     4021 tgttattcct tgcggcgggg atcacccttg ccgtttcacc ttcccatgcg gtttctctgt
     4081 ttggtatcac gctgatttgc gccggtttct cggtaggttt tggcgtagcg atgagtcagg
     4141 cgttaggacc attttcatta cgcgcgggcg tagccagctc gaccttaggt attgcgcagg
     4201 tttgcggttc gtcactgtgg atttggctgg cagcggtggt tggtatcggc gcatggaata
     4261 tgctgatcgg gattctgatt gcctgtagca tagtgagcct gttactgatt atgttcgtcg
     4321 cgcctggacg ccccgttgcc gctcatgaag aaatccatca ccacgcttga tctcaatctg
     4381 ctgctctgtc tgcaactgct gatgcaggag cgcagcgtga ccaaagcggc gaagcggatg
     4441 aacgtgaccc cttcagcggt gagtaagtcg ctggcaaagt taagagcgtg gtttgacgac
     4501 ccgctctttg tgaactcacc gctggggctg tcgcccacac cgctaatggt cagcatggag
     4561 caaaatctgg cggaatggat gcaaatgagc aatctgctgc tggataaacc gcaccaccag
     4621 acaccgcgcg gcctgaagtt tgagttggcg gcggaatcgc cgctgatgat gatcatgctt
     4681 aatgcgctgt cgaaacggat ctaccaacgt tacccgcagg cgaccatcaa actacgtaac
     4741 tgggattacg attccttaga tgccattact cgtggtgaag tggatattgg tttttccggt
     4801 cgcgaaagcc atcctcgctc gcgggagctg ttaagctcgc taccgttagc cattgattat
     4861 gaagtgctgt ttagtgatgt accctgcgtc tggttacgca aagatcatcc ggcactgcat
     4921 gaaacgtgga atctggacac ctttttacgt tatccgcata tcagcatttg ctgggaacag
     4981 agcgacacct gggcgctgga caatgtgtta caggagctgg ggcgcgaacg cacgattgct
     5041 atgagcctgc cggaatttga gcagtcactg tttatggcag cgcaacccga caatctgcta
     5101 ctggcgaccg cgccgcgcta ctgtcagtac tacaatcaac tccatcaact gccgttggtt
     5161 gctcttcctc tcccgtttga cgaaagccag caaaaaaagc tggaagttcc ttttaccctg
     5221 ctgtggcata aacggaacag ccataatccg aagatcgtct ggttacggga aaccattaaa
     5281 aacctttacg cgtcgatggc ataaccgaat cgtatgaaac gcgacccaat ttcacaataa
     5341 aatgtaaaaa agttgtaata agcttgtctg aatcgaactt ttagccgctt tagtctgtcc
     5401 atcattccag taaatgatta ctcttatatt cataatggac cattaagcat ggagcgaaaa
     5461 atggcgactc actttgcccg agggatttta acggaaggac atctgatttc tgttcgtctc
     5521 ccctcccagt gtcatcaaga agcccgaaac attccccctc atcgtcaaag ccgttttctg
     5581 gcgtccagag gtttactcgc agaactgatg ttcatgctgt atggcattgg cgaattgccg
     5641 gaaatcgtca ccctgccgaa aggtaaaccg gttttcagtg ataaaaattt gccttcgttt
     5701 tctatttcct atgccgggaa tatggttggc gtggcgttaa caaccgaagg tgaatgtggc
     5761 ctcgatatgg aactacagcg tgcgacgcgc gggtttcata gcccacacgc gcccgataac
     5821 cacacctttt ccagcaatga atcgctatgg atcagtaaac aaaacgatcc taacgaagcg
     5881 cgggcgcagc tcatcacgct gcgccgaagc gtgctaaaac taaccggtga tgttttgaat
     5941 gacgatccgc gcgatctgca gctgctgccc attgccggac gcctgaaatg tgctcatgta
     6001 aatcatgtag aagcgttatg cgacgcggag gacgtgctag tgtggtccgt ggcggtcacg
     6061 cccgcgattg aaaagctcag tgtctgggag ttagatggca aacacagctg gaaaagcctg
     6121 ccggatattc acagccgcgc caacaatcct accagccgga tgatgcgttt tgcccagctc
     6181 tctaccgtga agtctttttc gccaaattga tagacaacca caggagtcat tatgtctgaa
     6241 aaattgcagg tggttacgtt actggggagc ctgcgcaaag gctcatttaa tggcatggtt
     6301 gcacgtaccc tgccgaaaat tgctccggcg agcatggaag tcaatgcgtt accttccatt
     6361 gccgacattc ccctgtatga cgctgacgta cagcaggaag aaggttttcc agcaacggtt
     6421 gaagctctgg cggaacagat ccgtcaggct gacggtgtgg tgatcgtcac gccggaatat
     6481 aactactcgg taccgggtgg gctgaaaaat gccatcgact ggctttcccg cctgccggat
     6541 caaccgctgg ccggtaaacc ggtgttgatt cagaccagct caatgggcgt gattggcggc
     6601 gcgcgctgtc agtatcacct gcgccagatt ctggttttcc tcgatgcaat ggtgatgaac
     6661 aagccggaat ttatgggcgg cgtgattcag aacaaagttg atccgcaaac cggagaagtg
     6721 attgatcagg gtacgctgga ccacctgacc gggcaattga ccgcatttgg tgagtttatt
     6781 cagcgagtta agatctaaat aaaaaacccg ccagcaatca tgcatggcgg gtttttaacg
     6841 cgctatcgat tttagtgagc gtcgataaag acaatcttca ggataaacag cagcgcaacg
     6901 atgattacgc acgggcttag atcacgcaga cgtccggtac cgattttcat cacgcagtag
     6961 gagataaagc ccagcgcgat accttcggta atcgagaagc tgaacggcat catcacggcg
     7021 gtaataaacg ccggaacaga ttcagtaaga tcctgccagt tcacgcgtgc cagactggaa
     7081 gtcatcagca cgccaacgta aatcagcgca ccagctgcag cgtagcctgg caccatcccc
     7141 gccagcggcg acagaaagat aaccagcagg aacagcagac caacaaccac tgccgtcaga
     7201 ccggtacgac cgccaaccga tacgccggaa gaggactcaa tataagccgt aacggaagaa
     7261 gtaccgataa acgaaccggt cacggaagag atactgtcga catacagcgc ctgcttcatg
     7321 cgcgggaatt tacccttctc atccgccagg cctgctttat cggtcacgcc aatcagcgta
     7381 ccggaggagt caaacaggtt gaccagcatg aaagagaaaa tcacccctgc cagcccgagg
     7441 ttaaacgacc cggctaaatc tacatgaccc acaactgtca ttacgctcgg cggcgcagaa
     7501 acgatgccat tgtagtgcac atcacccagc atccagccca gcagcgtcgt caccacgata
     7561 gaaaccagca ccgctgcgtg aatgttgcgc gaggccagaa tagcaatgat gaagaagccg
     7621 aggataccca gaagtacgct gtgagaagtc agattaccga tgctcaccag cgtttccggg
     7681 ttagcgacaa tcacacctgc gtttttcagc cccatcatgc caatgaacag accgataccg
     7741 ctggtaatac ccacacgcag actcaccgga atgttggcaa tcatccagta gcgaacgcgg
     7801 aaaatcgtca gtaacagcag acctatcgcg ccccagaaga ttgcgcccat cccgacctgc
     7861 cacggcaagc ccatcgcctg tacaacgaca aaagcgaaga acgcattcag gcccatagcg
     7921 ggtgccagtg caactggcag gttagcaaac agtcccatca taatactgcc gaatgcagcg
     7981 atcagacagg tagtgacgaa gacggcgctg gtatccatgc cagcaacgcc aagaatttgc
     8041 gggttaacaa aaacgatgta aaccatcgtc aggaaggtgg taaaaccggc gatcacttcg
     8101 gtccgtgccg tcgtgccatg ttcgcgcagt ttaaacacgc gttccagcat cccctggcca
     8161 gaagcctggg ttgtgtgttg ttgactcatt atctatttcc gaacaaggag ggaaaatccg
     8221 tcgctatcgt ataccaaaat gagacaatat ggacggctga agataacttt ttttcattgt
     8281 atttgcttat acggcaacga ttgcgtcatg cgaattggca tgacgaaaac gttaaactaa
     8341 cgataaagga aagacatgtc ccagatagaa gcggtatttt tcgactgcga cggtacgctg
     8401 gtcgacagtg aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt
     8461 acgctcgatc ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc
     8521 gatattgttt cccttgaaca tggtgttacg ttagcgaaaa cagaagctga acacgtttac
     8581 cgtgcagaag tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctggagcg
     8641 ctcctgtcag cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg
     8701 cagcattcta tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc
     8761 tacgatattc agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg
     8821 aatgtaaatg tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt
     8881 atcgacgcag gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt
     8941 cacccgaaag tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt
     9001 ggttgggata ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg
     9061 ctcaaaggta agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga
     9121 ctttattttg cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc
     9181 ttcgcaaaag aaccgctggt tcttttgcga agaatacagg tacttccatt gtttctctta
     9241 ttgtcgatta ctacgggagt tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg
     9301 ccggaaaaga tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc
     9361 gttatcaccg agatctattg tgcagttatt gtacatatta agggcatggc ttcctcggag
     9421 ttgtatgaaa acattctttc tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg
     9481 gcaggtgtgg tgatgagcag aatcattacc cgtctgcccg gattggatat ttcatgtcct
     9541 gaaacagact ctttaagtta agcgggatac tttatctttg ggctactcaa aagcagacag
     9601 gatgttacta tgactcaaaa tatcaggccg ttaccccaat tcaaatatca tcccaagcca
     9661 ctggaaacag gcgcatttga acaggataaa accgtagagt gcgattgctg tgaacaacag
     9721 acgtcagttt attactcggg tcccttttat tgcgttgatg aagttgaaca tctctgtccg
     9781 tggtgtattg cggacggttc tgctgctgaa aagtttgcag gtagttttca ggatgatgcc
     9841 agcatagaag gtgttgaatt tgagtatgat gaagaggacg aatttgccgg tattaagaac
     9901 acatatcctg atgaaatgct gaaagagttg gttgaacgca cgccaggtta tcatggatgg
     9961 caacaggaat tctggcttgc gcattgtggc gatttctgtg cttttatcgg ctatgtgggc
    10021 tggaatgata taaaagatcg cctcgatgaa tttgccaacc ttgaagaaga ttgtgagaat
    10081 ttcggtatta gaaattctga tctagctaaa tgcctgcaaa agggtggtga ttgtcagggt
    10141 tatctcttcc gctgtctcca ctgcggcaag ctgagactgt ggggtgattt ttcgtagtta
    10201 tttaaataat gagaacaggc cggagcgtaa ttcatacatc cggccttatt tcttcagctt
    10261 aatcgagcgc taattctgcg gcagcggctt tatcagcgat taccatcagc gaaggatgca
    10321 gctgcaacac ggaagctgga acatcttcgg taaccggacc ttgcagcaca tttttcagtg
    10381 cctgcgcttt acctgcgcca ctgacaatta tcagcaagtt tttcgccgcc atgatgcttt
    10441 tcggccccat cgtcacgtag ctgtcaggca ctaacgaaaa gtcgccgccc aactcaccat
    10501 gcgccacaat atcaaccatc tctccctgga tcgggaactc cacggtttgc tcatggaaat
    10561 gggtcgtatt cggcaaattg ccacaaaaat gaccatctgc acctaatccc agcaccacca
    10621 aatccaggcc gccttcacgc gccagtttct gatcatgctc gcggtagtta tcaatagtga
    10681 gcttctggat attctcttct ttgatccccg caggggtgaa aaacagatta cgcagattgg
    10741 taatcgttac accttctccc tctttgccgc gaaagggaat ttcatcgaaa ttatagaaat
    10801 agcagttatc ataccagggc ttacctttaa ccagggtggt gaggtattca tacatgcctt
    10861 tgggcgtgct accggcggta attgccaggt taacacgacg cgtcttcgac atataaccaa
    10921 gcagatggtg tgccgcgaca cggctcattt cctggtaatc ttcggtaatg attaatttca
    10981 tcttagtgcc ttattttatt taaataattt ctgtacgaag gctgcatagg caggccgcca
    11041 gacatccatt tcatggttca ggccgggata ttcctggtaa tcaaacttaa tttttttctg
    11101 ctcaagctca gttttcagcc cggcgatatc cttgccggtt acgacatctt tatcaccaac
    11161 gaccacagta aaattacgta gttgctggtt aatagctgcc ggatcgttca gccgggccgc
    11221 gacaccttca tccggtacgg ttgtcgtggt aacaccactg aatgtggcca gccagccaaa
    11281 gctttccaga tgattcattc cggaaaccag cgcctggtac ccgccttgtg aaagccctgc
    11341 cagcgcgcga ccatcggcat ctttacggac attaaaacgc ttgctaatca gcgggataat
    11401 atcgttcatc agttcgcgat ctgccgcttt agcattcagc ggataaaaga ctttacgtct
    11461 ttcctgaggc acgaaatctt cgggaataat gcccttcgca tcggtttcgg tatccgggat
    11521 caccaccagc atcggtttaa ttttcccttc agccagcagg ttatccatga tttgcgggat
    11581 acgcccctga tcgatagcgg aacgtccggt atcaccaaaa ccgtgataga aatagagcac
    11641 tggcaaaggc tcgcccatgc cggtgtaacc tggcggggtc cagacataca tctgacgttc
    11701 agattgcaat gcgttggagt ggtaagttat ggcgatcaaa tcaccatgcg caacagaacg
    11761 cgtatccaga taactgcctg gcaccagaat catactggag ttaacctggc gctgaggctt
    11821 agtcattgcg gtgcctgtat caatgctgcg tacaccatca acattgaaaa aatactcgta
    11881 caggttgcct ttcaggatag gtgtgcgcca cgaccagact cctgcttcgt ctttggtcat
    11941 cgggtgaata ttgtccggaa ccggaacacc caccactaca gagacatttt ttgccccagg
    12001 ggcaaagtag cgaaaggtca cgctgttatc ggcattgacc tgagtcacat actgtttcac
    12061 cgggatagtg ggtgccgggc tggctggcat atccgctgct atggcccact gagcagaaat
    12121 accgctggca atagccagcg ttaaagcagc aatttttata ttcatcgtaa tattcctttc
    12181 attaagttcc attaccacca gatttcagcc tgggcaccga cagcgaactg gtcgtcttta
    12241 ttgtcttcga aggtgaagcc atccagttcg ttttccaggg ctttgatata cgtggcgtag
    12301 aaacgaattt ccggacgcga ggtcaacata ctggtattga ctttaaaggt atggaagagc
    12361 gtggttttat aaccagactc attaaattta ttactattcg catctttgtt ttgctgggtg
    12421 aaatagccca gttcaacacc tgtctggtta tattggtccc aaatataggc cgggcgaaca
    12481 accgcacgaa tagattcgaa atcagagtgt gcgcctgttt cgtagctata aatatcgtta
    12541 ccgaaggagt aaacaatcgc gttagccaca ataaaatgat cgccgatata ggcttcgccc
    12601 tgtgaagtca gacgaactgc tgttccgccg gtgtgatcac cataataacg accattaaag
    12661 gtggtaaatg gacttgcgcc agcataacgg ccaaagttac tggcgataga gttattcgca
    12721 accaggaagg agaattcgtt gaagccacct ttatcaaatt tctgcgttaa agatgtgcca
    12781 aacatccagg tatctttcca gtcataatag ccgttattat cctgattatc tttttccgat
    12841 gcgctttcgt ttgccgtgac ataacgacca ctcaccatta aggtcgcttt atcccataac
    12901 gggatatctt tatagcgtaa atcaatagta ttggtattaa tctgctgttt gttttgcagg
    12961 ctgcgatcgt aatcatcgac atcttcgcga accagcgcga tatcaatttt acccggaccg
    13021 actttccagt tttccagacc tacacccgct gcggcatcag tacgctgcgt tttccagtcc
    13081 agcatctgga tttcaatttt cggcgcaccg tgtttaccca cccagaaatc agcctctggc
    13141 gcaaagggca ggaaaccttt ggtggtaacg tacatatcgg agaactgcat aaagttctcg
    13201 ccaccggcgt tatcgccaaa ccagccggta gagtactgct gaccaacgtt accatccatc
    13261 atcacaacgg catcaacccg tttgccgttt tcgttgtaga cacgttgttt taactgcaaa
    13321 tcaaaccagc cggagtattc gttaccaaag cggcccagag aaccaatcgc ccatgattta
    13381 ggtgaaccat gagaggcggt cccccaacca gaacggtaat agccgttgta actaaagcca
    13441 atttcatctt tcacaaactt actgaaatct ttcagagtca tggaagaata aatcgagcct
    13501 gtctcactgg catttttctc ttcatttttg accagtacag catcaggttt tgcggcacta
    13561 ctggtcggga acggattggc ggcataccct tgatcattcg tacttacgct acgattcacc
    13621 gtcgctggcg tatatttttt cttctcttca tctttatact ttttgagttc gctttgcgtt
    13681 tctcttaacg ctttttctaa taactcaagg cgatgttcca ccgttaatga ttcagccaat
    13741 gattgtgcag aaaaggctaa cggtgccatt aataagatgg cagaggtaat aagatttcgt
    13801 ctaaacataa tatcccttta tggtgcaaag aaagaattaa cgtatcgcat ccagactgtt
    13861 ctgaatgcga cgataattaa ggtgctttat tggttatttt tttaatgaca gcccccgcgt
    13921 tttgatcact tctgcatacc atccgaagct tttcttacgc gttcttgtga ggcttccttc
    13981 gccattatca tcacgatcca cataaataaa gccgtagcgc ttagacattt gtgaatgaga
    14041 tgcactgact aaatcaattg gcccccaact ggtgtacccc ataatatcca caccatcggc
    14101 aatcgcttca tttacctgta ccaggtgatc gtttaaatag gcaattcgat aatcgtcctg
    14161 tatcgaacca tccgcttcaa cgctgtcttt tgcgcctaat ccgttctcga caataaataa
    14221 cggtttttga taacgatccc aaagcgtatt taacagaacc cgtaatccaa ccgaatcaat
    14281 ttgccacccc cactctgaac ttttcagatg cggattgggg atcatattca gtatgttgcc
    14341 ctgcgcattt ttattaatgc tttcgtcgtg ggaaacacaa ccagtcatgt aataactaaa
    14401 agagatgaaa tcgacggtat gttttaaatc ttctgcgtca ctttcagtca tctcaatggt
    14461 gatattgtgg tcgcggaaga aacgctgcat atagccggga tactggccac gcgcctgaac
    14521 atcaccaaag aacatccagc gccggttctc ttccatggcc tgcaacatat cctgtggctg
    14581 gcaggtgagg gggtaaacca gcccaccgag aagcatattg ccgattttcg cttcggggag
    14641 caggctatga caggctttaa ctgcccgcgc actggcaacc agttgatggt ggatagcctg
    14701 ataaacttcc gcctcgccac tctcttctgc cagccccacg cccgtgaatg gctcgtgtaa
    14761 cgacatgttg atttcattaa acgtcagcca taacgccact ttatgttggt agcgagtaaa
    14821 gaccgtgcgg gcgtaatgct cgaagtgatc gatgaccgct cgattagccc aaccgccgta
    14881 gtttttcacc agcccatatg gcatttcgta atgggataac gttaccagcg gcttgatccc
    14941 cgcctgcgcc atttcatcaa acagccgatc gtaaaacgct aaccccgctt cattcggttc
    15001 gacttcgtcg ccctgaggga aaattcgcgc ccaggcaatg gaaatacgca gacaggtgaa
    15061 gcccatctcg gcaaataacg cgatatcttc cgggtaacgg tgataaaaat cgatggcgac
    15121 atctttgata ttctctttcc ccaggatgcg cggttccatt tttcccatta cgccatgagg
    15181 ctgtaaatct gaggtcgaga tccctttgcc atcttcctgc caggcacctt ccacctgatt
    15241 ggcagctgtt gcgccacccc aaagaaatgt ttctggaaat gctttcataa ttaactcctt
    15301 ttatcgttag cgaatgatgg ataacagcgg ttcacctgcg cttatctgcg ccgtgccgtg
    15361 gggtaatacg tccgtaaaat catcgctatt actgattaat accggcgtcg tcagatcaaa
    15421 tccggcctcg cgaatagcag ggatatcaaa agaaatcagc cgatcgcctg tattgacctt
    15481 gtcacccacg ttgacgtgag cggaaaagaa tttgccgtcc agttttacgg tgtcgatacc
    15541 gacatgaatc aggagctcca caccatcatc tgactcaatg ccaatggcgt gtaatgtggc
    15601 gaacaacgaa gcaattcgac ccgcaaccgg agaacgcact tcaccaaccg agggcagaat
    15661 ggcgatacct ttacccaaca ggccactggc aaacgtggta tcagcgacgt gaatgagcgg
    15721 cacaatctct cccgtcatcg gtgaacagat accgccctgc tcaggtggtg taataacctc
    15781 tggtgttttc tcttgcgggg caccctgcgc tggctgacgt ttagcggtga tgaaatgaag
    15841 catcaccgta ccgacaaatg cgcaaccgat ggcaatgaca ccgccaataa cgctggccca
    15901 gacggtgaaa tcaattcccg ttgacgggat ggtttgcatg aaggtgaaaa tacttggcaa
    15961 accaaaggag tacactttcg tttgcgcgta gccaatgatg gtggccccca aagccccact
    16021 gatacaggcg ataacaaagg ggtacttacg cggcagatta acgccatata ccgctggttc
    16081 ggtgatacca aacaggcccg tcaacgccgc tgatcccgcc accacttttt tctgcgcatc
    16141 gcgttcgcag aggaagacgc cgagcgccgc cccgacctgc gccataatgg cgggcattaa
    16201 cagcgggatc atggtgtcgt agcccagcac ggtgaagtta ttgatacaca gcggcaccag
    16261 gccccagtgc agtccgaaca tgacgaagat ttgccagaag ccgcccatta ccgcgcctgc
    16321 aaatgcagga accgcctgat aaagccagag ataaccggcg gcaatcagtt cgcttatcca
    16381 ggttgatagc ggccccacca gcagaaaggt gacgggtgtg ataaccatca gacatagcaa
    16441 tggtgtgaag aaatttttga ttgccgacgg taaccacgca ttaagtcggc gttccagaat
    16501 gctgcacaac caggcagaaa aaataatggg aataaccgat gacgagtaat tcaacaatgt
    16561 gaccggaata cccaggaaat ccagccccag ctcatccgct ttttgcccgt tctcgaaagc
    16621 agtcagaatt aatggatgca ctaacgctcc accaatcacc atggcagtaa atggattacc
    16681 gccgaagcgt ttccccgcgg tgtatcccag gattatcggg aagaaccaaa acaaggcatc
    16741 actggcgctg aataaaatta aataagtacc actttgttcg gtcgtccact gaaaagtgag
    16801 cgccagagcc agcatacctt tcaggatccc ggttgccgcc atcaaaccga tcagaggcgt
    16861 aaaaatacct gaaataacat aaacaaagcg gtttagcaga ttacctttct catcattttc
    16921 cggtgcctgt tgcgcttttt cgccaaggcc tgccacgctg ttgaccgcca ggaagacatc
    16981 ggccacatgg ttacctatga ccacctgaaa ctgaccaccg ctttccacca ccataataat
    17041 accgggggtc tttttcagta cctcagcttg cgctttgctt tcatccttta acttaaaacg
    17101 taatcgcgtt gcgcaatgca tcagactcac aatgttatct gcgcccccga ctcctgcgac
    17161 tatttttctg gctaactccg tcataacttg ccctctaccg ctttgcggca aaactccaaa
    17221 aaaaaacctg aaaaaaacgg cctgacgtga atcaagcaat ttttttcagg ttttgcccgc
    17281 ttagtgcggt aacaatcctt tactcagtaa taatatttca gtgttctttg cgcacgcgct
    17341 ctatatttat ggctaaaaac ataatctctg cgggtgaaat tttacgttga tactgcaaac
    17401 caataaaaat ggcgatccgt tccgcacatt gccatgcttg cgggtaattt tgttttactg
    17461 cttgttgtaa tgattcatca ctatcgttaa ttgaagcatg ttcaagaata cgccaggata
    17521 aaaacttcag atgtgtaacc agtcgctgat aactcaagct ttcttcctgg taattaaggc
    17581 tgaactgaaa ttttattaat tgcagcattt cgcgcattaa ctgcgtgaca cctgcaacat
    17641 cctccatatt tccgctcatt tgggcactga ccagatgcat ggcaataaag cccacttcat
    17701 ctttcggtaa ctgcacgccc aaccgtttat caataatggt taacgcttct tcccctagct
    17761 ggaactcttt cgggtaaagc cgctggatat cccacagcaa cgggttgggc agcaacacgt
    17821 tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt
    17881 cctgtaattt tcccaagcgc tcctgcgcta aagagataat acgatcacag gttgccatca
    17941 cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca
    18001 aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc
    18061 caattcctcg ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat
    18121 tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa
    18181 aaacccgact tcaccagtat tctctggtta tgtcaggttt tgcctgcgaa tgcagtaaca
    18241 atccagtcat ttattaatgg tttttataac gaacatccag gttcggaaat taatttaatt
    18301 gcgtgcagag aaaatagcaa tgcgctattg ataaaaatat gaccatgctc gcagttatta
    18361 actttgtgta attttaggaa tttataaagt tatatataac aaatcccaat aattaagtta
    18421 ttgggatttg tctggtgaat tatttgtcgc tatcttttcc cgccagcagt ttatccagct
    18481 catcaccgcc tacatgacgg aaatcctgcc ccttcacgta gtagaagata aactcacaaa
    18541 tattctggca gcggtcgcca atacgttcga tagaacgcgc gcagaacagc gcagtaagta
    18601 cgctcggaat ggtacgcgaa tcttccatca tgtaggtcat cagttgacga acaatacctt
    18661 cgtattcctg atcgactttt ttatcttcac gataaatacg taccgcttcg tcaatgtcca
    18721 tccgcgcgaa cgcgtccagc acgtcgtgca gcatctggat ggtatgacgg cccagcgact
    18781 ccagacttac cagcaacggc tgatgctgct gggagaattt ctccagcgca gtacggcaga
    18841 ttttgtccgc cacgtcgcca atacgctcca gctcggcaat ggttttactg atcaccataa
    18901 ccagtcgcag gtcgctcgcc gtcggctgac gtttggcgat aatgcgcacg caggcttcat
    18961 cgatcgccac ttccatcatg ttgacgttct tgtcgccttc gatcacgcgc ttcgccagat
    19021 cgctgtcctg gttatgcatc gcggtgattg catcagaaag ctgctgctcc accatgccgc
    19081 ccatggtcat cacctgcgtg cggatacttt ccagttcggc gttgaactgg ccggaaatat
    19141 gtttattaag attgagactg tccataacgc actcctgaat caaccgtaac gaccggtgat
    19201 gtagtcttct gtttgtttct tcgctggctt ggtgaacaga tcgtccgtgt tgctgaactc
    19261 aatcaattcg cccaggtaca taaacgccgt gtggtcggaa caacgcgcag cctgctgcat
    19321 gttgtgggtg acgatcacca cggtgtaatc ctgcttcagt tcggtgatca gctcttcaat
    19381 acgcccggta gagatagggt cgagcgccga acacggttcg tcgagcagca gcacttccgg
    19441 gcgaatggcg ataccacgcg caatacacag acgctgttgc tgaccaccag agagagagta
    19501 accgctctgg tgcaatttat ctttggtttc gttccacaat gcggctttgg tcaatgccca
    19561 ctgcacgcgc tcgtccatgt cggcacggga gagcttctca aacagacgaa cgccaaaagc
    19621 gatgttgtcg tagatggaca tcggaaacgg cgtcggtttc tggaacacca tgcccacttt
    19681 cgcacgcagc agggcgatat cctgagagtt ggtcaggatg ttgtcgccat caagcagaat
    19741 ttcaccttcc gcacgctgct ccgggtacag ttcaaacatt ttgttgaagg tacgcagcag
    19801 cgtcgattta ccgcagccgg acggcccgat aaacgccgtt acctggtttt tagcgatatc
    19861 caggttgatg tttttcaggg catggaattt gccgtagtag aagttcaaat tacgaacctg
    19921 aattttactc ggggcagttt caaccatact cattgcaatc ttttcctcat cgtgcctgat
    19981 gcacttcgct aatcaggctt accgtaggcc ggatcaggcg ttcacgccgc atccggcaaa
    20041 gtgttaaccg tgtttattct tcgcaaaaac aacgcgcgcc agaatgttca gcagcagtac
    20101 gcacagggta atgatcaata ccccggccca ggccagttgc tgccattccg caaacggact
    20161 catcgcaaac ttaaagatcg tcaccggcag gttggcgatc ggctgcatca tgtccgtgct
    20221 ccagaactgg ttggagagcg cggtaaacag cagcggcgcg gtttcaccag caatacgggc
    20281 aatcgccagc aagataccgg tcatgatccc ggacaccgac gctttcagcg taatcgcgga
    20341 gatcatcttc cacttcggtg tacccagcgc ataagccgct tcacgcaggc tgtccggcac
    20401 cagtttcagc atgttctcgg tggtgcggat aacaatcggc acctgcaaca gcgccagcgc
    20461 aattacgccc gcccagccgg agaagtgctc catctgtgcc accacgatgg tgtagacaaa
    20521 cagaccaacc acaatcgacg gcgcggagag cagaatgtcg ttaatgaagc gaatcacttc
    20581 tgccagccag gatttacgac catattccgc cagataaatc cccgccataa tgcccagcgg
    20641 cgtaccgaat accgtggccc acaaaattaa cagcccgcta cccgccaggg cgttcgccag
    20701 accaccacct tccgtattgg gcggcggcgt catttcagtg aacagcgcca gcgacatacc
    20761 gtcgataccg cgagtgatgg tggacattaa aatccagatc agccagaaca ggccgaaggc
    20821 catcgtcgcc atcgagagcg tcagcgcaat acggtttttg aggcggcgac gcgcctgcat
    20881 tttgcggcga gattcagcca gcgccgcagt ggtttgcatt tcaaccatag ccattagcgt
    20941 gccccctcat tcttagccag gcgcataatc ataaacttcg atgcggcgag gacgatgaag
    21001 gtaatcacaa acaggatcag gcccagttcc atcagtgcgg caacgtgcag accggattcc
    21061 gcttccgcaa attcgttcgc cagcgcagag gtgatactgt tgcccggcat atacagcgag
    21121 gcgctgtcga gctggtaggt gttaccgatg ataaaggtca ccgccatggt ttcaccgagc
    21181 gcgcggccca gccccagcat gatgccgccg ataacaccat ttttggtgaa cggaagaacg
    21241 atacgccaga taacttccca ggtggtgcag ccaataccgt aggccgactc tttcatcatc
    21301 accggggttt gttcgaacac atcacgcatt accgccgcaa tgtacggaat aatcatgatg
    21361 gcgaggatca cgcctgccgc gaggataccg ataccaaatg cggggccaga gaacagcgcg
    21421 ccaacaatcg ggatattcga catgatattg ccgaccggct cctgaaagta aacggcgaac
    21481 agcggcgcaa agataaacag gccccacatg ccgtaaacga tacttggaat ggctgccagc
    21541 agctcaatgg cgatacccag cgggcgtttc agccagccag gcgcaagctc agtcaggaac
    21601 agggcgatac cgaaactcac cgggacggcg atcagcagcg cgataaacga agtcaccaac
    21661 gtaccgtaga tcggcaccag cgccccgtag atatcgttcg gtgcatccca ctctttggtc
    21721 cataggaaag ccagaccaaa tttctgaatg ctcggccagg aggagatgat cagagagaca
    21781 ataatgccac ccaacatcaa tagcacaatc agcgccgcca gttttaccag cacgctgaaa
    21841 attatgtcgc cctttttacc cggtgggtta aaagcaggct tggttgcagc cataagttac
    21901 tcttcagtta aacgcgttta caaagtcatt ttgtaatgcc ggatgcggcg taaaacaccg
    21961 tacccggcct ggagttttat tagtacagcg gcttaccgct actgtcttta atattggtct
    22021 tccacgcagc gcgaacctgt tcaactacac tatccggcag gctggcgtaa tccaggtcgt
    22081 tcgcctgttt agccccggtt ttgtacgccc agtcgaagaa tttcagcact tctgtgcctt
    22141 gttctggttt cttctgatct ttgtggatca gaatgaacgt ggtagaggta ataggccatg
    22201 catcttcgcc tttctggttg gtcagatcct gagcgaaggt tttgctccag tctgcacctt
    22261 ttgctgcatt agcgaagttt tcttcggtcg gactaaccgg tttaccatca gcggagatca
    22321 gtttggtgta cgccaggttg ttctgcttcg cgtaagcata ttcaacataa ccaattgcac
    22381 ccggcagacg ctgaacgaac gcggcgatac cgtcgttacc tttaccgccc agaccgatcg
    22441 gccattttac ggtagagcca gtaccaacgt tgtttttcca ctcttcgttc actttcgcca
    22501 ggtagctggt gaagacgaag gaagtcccgg agccatctgc gcggcgtact acagcaatgt
    22561 tttgtgaagg cagtttcaga cccggattca gtttggcgat ggcttcatca tcccacttct
    22621 tgattttgcc caggtagatg tcgccgaggg ttttaccatc cagcaccagt tcgccagact
    22681 tcagccctgg aatgttaacc gccagcacca cgccgccaat cacggtcggg aactggaaca
    22741 gaccttcctg agccagtttt tcgtcagaca gcggcgcgtc agaggcacca aaatcaacgg
    22801 tattagcgat aatctgtttt acgccaccgg aagaaccgat accctggtag ttaactttat
    22861 taccggtttc tttctggtaa gtgtcagccc atttggcata caccggcgca gggaaggttg
    22921 cacctgcacc tgtcaggctt gcttctgcaa acacagagaa agcgctcatc gataaggtcg
    22981 cggcgacaac agttgcgacg gtggtacgca taactttcat aatgtctcct gggaggattc
    23041 ataaagcatt gtttgttggc tacgagaagc aaaataggac aaacaggtga cagttatatg
    23101 taaggaatat gacattttta tgacagaaag gaattaccaa ataacttact gattttaaat
    23161 gtgaagatga aaatcccaca tgtcatcttt ttgtcatgat gcatttaaaa gtgataaaga
    23221 aatgggtgag atgacaacga tgtgatgaac aatcgttgtc agacctatta gcgcacaatc
    23281 tcaacagtta tcgtcgctgt tgcagtaaat tgccccgggg atgggcgagc tccagtaagg
    23341 ctcgcaggag cagctgaaaa agttacacta cctgattttt caccatacac tgttgattta
    23401 cccatgtctg ttggcaactc accaccgttc actttgattt ccttattatt tttgtcgtaa
    23461 atttttatcc ccacgttaga gtttgaagtt acgaccattg aattgtccgt attgggttcg
    23521 gtagcataaa aagacgtact gatatcatcg gtattcagta tccccaggtt ttcacactca
    23581 atagtgaccg ttttagtaat tttatgtgtt gttaccgcac ttccaacaac agatgaaaaa
    23641 tctgcagctg gtatttcttt aaaatcaaaa taaactggct gtaaattatt tatttcgcaa
    23701 ctttgcggag ctgtaatatc gccccgaata taaacatcag ccatcggctg tgagtaatcc
    23761 accggggtgt cacggctaat tgttccatac aatgtcacta tctttgtttc aggaataatc
    23821 aattttcctg caaatgtctt cttgatataa aaatatacta tcgctttagc cccactggat
    23881 atacttaaag gttcaatgcg gttgcaattg tactttgtat taccagacgg gttgttagca
    23941 tgatattcaa aaggtgcatt cacataccca accccaagaa tatctatagc aatcccaaca
    24001 tcaagattcg gattaagatc ataataatta atattagagt gaaccccagc ataagttaat
    24061 gcctggttta tttttgacgt ataatagata taggggaatt ctcctacgtt accagtatca
    24121 caatgacagc gtccaggata tgaggatcca ttagttacag tggtgttaaa tgtcgttcca
    24181 accttatttt cgttcgcggt aataagatat cccgagaaat caagcgtact gtgaaatacg
    24241 ccattatcag gggtacagat acctttagga gctgcaagtg ctacactact taacgagagc
    24301 ataaacacag caaagcacca ctgtttgata tacttgttca taaaaatcct tttaatgaca
    24361 tgtcacaccc tgttcaatta atccgctctc actttctggt gtcgaaagtg aataattgaa
    24421 gatgcatttt gttgaggcgt tttctcccca ggataacgta acctgtcctt tttctggtag
    24481 accagaaaga taaagttcgc cacggtcacc gacaattcct gaatctgcca aacttgcatc
    24541 ttgtatcgca gcaatggctc caaaaggtac cggtttacca tttgcattta acactctgaa
    24601 gagaacgcga taacccacat gagtcacaaa ctccgcttta accactgcac cacgcgtgga
    24661 aaccatcgta acgacggtat tatcaagttc tatattcgac ttcgagaaat agttatcgct
    24721 gattgctacc cgattttcat cataagggtt tagctgtgtc ttgaccgtat agccgcgcca
    24781 gtccgtcgca gcaccgcgca tattatctat ttctaacccg gcggccccag gtgctttaac
    24841 aagagcaata gtttcaccta actcctgact taacgttacc ccttcgctgt gtgccagaat
    24901 tccccctctg ataccataat tcagcctctg ggaattttgg ctgtagttat agctgccgtt
    24961 caccgtgcca taagagccat cataggtcag ggacatatta ccgccaacct catcatcatg
    25021 actgttatag gactgcatta aattccatga aaggttattt cgttccagcg ccgtaccgct
    25081 gagtccgagt tgatgtgagc taccggaatt atcgttttgc gaaacagaat aggtggcgta
    25141 actattaccc caccagcccg ataaaggaac agataaagag agcatgtaga tctgctcact
    25201 ggtgttatca ctggcatggt tgttccccgt cccctgtctg gcagtattgg aataactcca
    25261 ggagacgttc cacgagagac gcttccagct actgctgtag ccaaattgca ttaatcgttc
    25321 gacatcatcg gtatgccagt attgttgccg gcttaatgtc aggtataaat tgccataatc
    25381 tctccccaac gactgcgtta gcgttccctc cacgcgacta cgtcggttac ctgtttccca
    25441 aaagtcttca gggctgttgc gccgcgatgc ccactcattg agggtatagt agccctcggt
    25501 ggagtaacga tagccaacca ggcgcaagct agtgtctgtg tcgtcaaatc gttttgaata
    25561 gagaaagcga tatgactgac ccgtttctgt atcatcatgg ctaaaattcg cacgagcatg
    25621 tgtcacatcg aaagaaattg cgcccaaagc gccaagatct ttaccgacac ccagagctgc
    25681 agaaccataa ttttcagcag cctgcatacc tccatataaa gtgatcccgt gggaaaaacc
    25741 atgaagtatt tgcgcctgaa ggacgtccgg tgtaaatccg tcttcatcgc gcaattcacc
    25801 cacgctgaca tcaacatctg tctgaccttc acgtttaaga atcgccaatg aagcataagg
    25861 ttgggtgaat cgttgttcgc taccgtcttc ttctttgatg gttacgtcca aatcgccacc
    25921 tgtactggcg gtgttgagat cggtgatctc aaaagcacct tgtggaacgg cagactgata
    25981 aatcacgtag ccattctgcc gaatgatgac ggtggcgttg gactttgcaa tcccacgaac
    26041 cacaggagca aagccattct ggctggcggg tagcatatcg ttatcagtat acaaacgcgc
    26101 gccgcgaatt tgcgtactgt cgaaaatatc gctcgccgtc cacgtatcac caatcataat
    26161 ctggcttctt aacgcggcaa tatcgcgctg tatccaggta ctaatggagt tgtattccgc
    26221 acggccatcg ctggtgttta aagtactata attacgtagc cgccacggtc caatattcag
    26281 tccattgcgc agattgagat aactggtatc tgtctcttgt gaaccataat ctgaacgtag
    26341 tgtatgtgaa ccagaaaaat cgtagttgat ggtgagcgcg ttaatcccct catcccaacg
    26401 cgtggggctg atgtaatcat aagccatcgc gtccatgtag atttggggaa tggatatatt
    26461 cagctgcaat ttatcactgt caaactgcca ggatgagcct tcaatacgtg gagcaggatc
    26521 gaggcaaacc gtctcatcca catcattgaa cgatttgagc gcatcaactt ttatgccaaa
    26581 ggaggacagt gtttttttaa tggcaataaa gcaagcgtgc agatcagcat ctggattatc
    26641 ctgaacaaat gtcacatcct gacgttcaaa cattttaccg ttgataaaaa tatcaacgct
    26701 ataaacacct gggggcatca ggtttgcccg attaaactgg cgaatatcga ctccggattg
    26761 atggtcgatt tccagaagcg ccggattgaa tacactgacc tcggccatca ggttttgcga
    26821 gaaaatcaga cacgtccctg ccgtgaggcc aaccactatt ctggtcgtca tcataatacg
    26881 cctatcctta tcccttgctt acagcgtcat ttttatcggg tcactgactg cgccgtaatc
    26941 attaataaat cgccaggtca tttggcctcc cgcagaatta gcaggtaaag gtaacgtgag
    27001 cgtttcaaaa ggacggatca tattgagggg cacatcatgg ccatcaacga tgacatccat
    27061 cacactgata tgaattgggt tgggattctc tgctatcaga tttcgccctt cccggcgcca
    27121 tttcactttc ggcgcagcat cttcgggaat aaggcggata tttgctggtc gccagaacag
    27181 cttgatacgc gtttttactg ctatttccag cgagttcgtc gctttgttgc tcgctgatgg
    27241 cggaatattt ttaatattca gccagaagac tgactcgcga tcatcaggca atgacacacc
    27301 tttggtgtga ataatgcgta atattttttc ttgcgaaggt tctaatcgtg atactggcgg
    27361 aataacggta aacggcgctt tgcttttatc ctctttatta aat
//
LOCUS       DAZGLJ010000041        27378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000041 DAZGLJ010000000
VERSION     DAZGLJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.041"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(363..1025)
                     /gene="ytfE"
                     /locus_tag="ACHK49_001570"
     CDS             complement(363..1025)
                     /gene="ytfE"
                     /locus_tag="ACHK49_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO9262239.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(1133..2098)
                     /gene="ytfF"
                     /locus_tag="ACHK49_001571"
     CDS             complement(1133..2098)
                     /gene="ytfF"
                     /locus_tag="ACHK49_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO9262240.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(2206..3066)
                     /gene="qorB"
                     /locus_tag="ACHK49_001572"
     CDS             complement(2206..3066)
                     /gene="qorB"
                     /locus_tag="ACHK49_001572"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO9262241.1"
                     /translation="MIAITGATGQLGHYVIESLKKTVPVSQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            3155..3535
                     /gene="ytfH"
                     /locus_tag="ACHK49_001573"
     CDS             3155..3535
                     /gene="ytfH"
                     /locus_tag="ACHK49_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO9262242.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(3664..5607)
                     /gene="cpdB"
                     /locus_tag="ACHK49_001574"
     CDS             complement(3664..5607)
                     /gene="cpdB"
                     /locus_tag="ACHK49_001574"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO9262243.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            5797..6537
                     /gene="cysQ"
                     /locus_tag="ACHK49_001575"
     CDS             5797..6537
                     /gene="cysQ"
                     /locus_tag="ACHK49_001575"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO9262244.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(6527..7084)
                     /gene="ytfJ"
                     /locus_tag="ACHK49_001576"
     CDS             complement(6527..7084)
                     /gene="ytfJ"
                     /locus_tag="ACHK49_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO9262245.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            7409..7615
                     /gene="ytfK"
                     /locus_tag="ACHK49_001577"
     CDS             7409..7615
                     /gene="ytfK"
                     /locus_tag="ACHK49_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO9262246.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(7677..9020)
                     /gene="paeA"
                     /locus_tag="ACHK49_001578"
     CDS             complement(7677..9020)
                     /gene="paeA"
                     /locus_tag="ACHK49_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO9262247.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(9343..9981)
                     /gene="msrA"
                     /locus_tag="ACHK49_001579"
     CDS             complement(9343..9981)
                     /gene="msrA"
                     /locus_tag="ACHK49_001579"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO9262248.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            10187..11920
                     /gene="tamA"
                     /locus_tag="ACHK49_001580"
     CDS             10187..11920
                     /gene="tamA"
                     /locus_tag="ACHK49_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO9262249.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11917..15696
                     /gene="tamB"
                     /locus_tag="ACHK49_001581"
     CDS             11917..15696
                     /gene="tamB"
                     /locus_tag="ACHK49_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO9262250.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTVQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     NRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            15699..16040
                     /gene="ytfP"
                     /locus_tag="ACHK49_001582"
     CDS             15699..16040
                     /gene="ytfP"
                     /locus_tag="ACHK49_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO9262251.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            16252..16503
                     /gene="chpS"
                     /locus_tag="ACHK49_001583"
     CDS             16252..16503
                     /gene="chpS"
                     /locus_tag="ACHK49_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFO9262252.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            16497..16847
                     /gene="chpB"
                     /locus_tag="ACHK49_001584"
     CDS             16497..16847
                     /gene="chpB"
                     /locus_tag="ACHK49_001584"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFO9262253.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(16927..17457)
                     /gene="ppa"
                     /locus_tag="ACHK49_001585"
     CDS             complement(16927..17457)
                     /gene="ppa"
                     /locus_tag="ACHK49_001585"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO9262254.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            17767..18723
                     /gene="ytfQ"
                     /locus_tag="ACHK49_001586"
     CDS             17767..18723
                     /gene="ytfQ"
                     /locus_tag="ACHK49_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO9262255.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            18863..20365
                     /gene="ytfR"
                     /locus_tag="ACHK49_001587"
     CDS             18863..20365
                     /gene="ytfR"
                     /locus_tag="ACHK49_001587"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO9262256.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            20379..21401
                     /gene="ytfT"
                     /locus_tag="ACHK49_001588"
     CDS             20379..21401
                     /gene="ytfT"
                     /locus_tag="ACHK49_001588"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO9262257.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            21388..22383
                     /gene="yjfF"
                     /locus_tag="ACHK49_001589"
     CDS             21388..22383
                     /gene="yjfF"
                     /locus_tag="ACHK49_001589"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO9262258.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(22416..23414)
                     /gene="fbp"
                     /locus_tag="ACHK49_001590"
     CDS             complement(22416..23414)
                     /gene="fbp"
                     /locus_tag="ACHK49_001590"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO9262259.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            23590..24963
                     /gene="mpl"
                     /locus_tag="ACHK49_001591"
     CDS             23590..24963
                     /gene="mpl"
                     /locus_tag="ACHK49_001591"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO9262260.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(25119..25670)
                     /gene="yjgA"
                     /locus_tag="ACHK49_001592"
     CDS             complement(25119..25670)
                     /gene="yjgA"
                     /locus_tag="ACHK49_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO9262261.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWHDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            25764..27116
                     /gene="pmbA"
                     /locus_tag="ACHK49_001593"
     CDS             25764..27116
                     /gene="pmbA"
                     /locus_tag="ACHK49_001593"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO9262262.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            27299..>27378
                     /locus_tag="ACHK49_001594"
     CDS             27299..>27378
                     /locus_tag="ACHK49_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="electron transport protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO9262263.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLE"
BASE COUNT         6550 a         6865 c         7460 g         6503 t
ORIGIN      
        1 tgccagaatg gacggacagg aaaatggagg gggagtataa aagaggcgag aaattccatg
       61 tgctgtggtc gctgatgaac cgccatgatg gcgggtcggt gtgggtggaa gcgtagcggt
      121 agctggatgt acacgtaaaa accagggaat gggtgatttt attctggctt atacaggagc
      181 gatgaataag taattatatt aatgaaaaaa ataagttagt aaccgttgaa ggaacttatt
      241 attggctgaa taatgaattt tcccagttca gtagatacaa aagattgtcc cggagtggtg
      301 aataactcaa agcatttgaa aacgcacctg attgtgtaaa ccaggtgcgt tgatcgaaca
      361 aattactcac ccgccagcgc gcgtgggaac agtacattgt tttccaggct gatgtgatcc
      421 atcaggtcat caatcagttc attaataccg ttatacatcg ctttccaggt ggtgcaggct
      481 tccggcggcg gtgtgacgtt attggtggtg tgtttaatca cttccagcag ttcgcccgct
      541 tcatcgtgct cgctttccat cacgctgatt ggtcccattg cctggctgcc catgccttgt
      601 ttgatcatcg ggaagaggat ctgctcttct ttcatcatgt ggctggaaag ctcttcatgc
      661 agcatagtca ggtattttgt cagccctttt ggcacgctcg gtttgtcggc gtgaacgcgc
      721 tcgactttag tcgcttgcag gatcagctcc ggcagctgct cgcggtgacg atcgtggtag
      781 cgcacgatga tatggtcaat gatttctgcc agcggggcgc tacgccagtc tttctcaatc
      841 ggttgttcag caagctttgc cagttcagct tcaatgacct caacatccag ttctttacgt
      901 gccgccgcgc gcgccagcgt ctgcttaccg ccacagcagt aatccatatc atatttacga
      961 aacagagccg aagcgcgagg gatagagagc gccagttcac ctaaaggttg gtcgcgataa
     1021 gccatagctg atacctcatt cttaataaga taagatgtat tttaaatgca tctttaaggc
     1081 aaaagctata acccttacgt agcagaggct tatttaactc actgccagcg tgtcaacttt
     1141 ctgagacaga ttcagttaaa ggtttttctg gttttgctct gaccgcaatg accacgccaa
     1201 tcactaacag cgcgataccg ctcagcgtca ttagcggcgg catttgctgg cggagtaaaa
     1261 aggtgtacag caaacctgcc agcgtttcga aaacaatcag cggcccgaga atcactgtcg
     1321 gtaatcgctg gctggcgacg ttccagcaga gtgcgccaac ccatgagcaa agcacggcta
     1381 tcgcaaccat cagactaata aacaccagcg gacgggggcc aaaaggtaag gagaagtccg
     1441 gcgtttgtat attcagccag taacaggcga cgagatagcc gatgagagaa accggcagcg
     1501 tgaccagcgc ctgcgccgtc gcccacatca tcggatgttt gtcgggattt tcccgcagcc
     1561 agcgggcgtt gcgcagggca taccatgccc agcagaccac ggaaactaac gctagcacga
     1621 tgccagaggt ataacgtgcc cagtcaaaat cggggagtcc gtggtttaac tcagcaatat
     1681 tcacactcgc caggccgatg ccaatacaaa tcagtgccgg ggcgagtttt ccccacgcga
     1741 gtttgccgtc gcgctggcta taaagcagat tggcaaagac aggaatgacc accggcaggg
     1801 tgccgataat catcgtggaa acaggcgcgc cagtacgttg aatggcactg gcaaggcaga
     1861 agtaataaat gaggttgccc atcatagtga gcatcaaggc ggtaagccag tcccgacgcg
     1921 ccaactgacg cagacgcacg cgtcccagcc aggcaatggg cagcgcaatt aaccctaacg
     1981 ccagataacg ccccatcgac tgcaacatcg ccgggtattc cggtacgatc aacggcccga
     2041 caaaaataag cccccacatc aaccctgcta acagggcgta cagcacgccg ctaatcatta
     2101 ctggcatcca ttggtctgtc agaagaaagt tcaggaagca gacatgattg ctcccggcat
     2161 cccgtcatta ttagagagga tgcgggagga tgccacttta attaactagt tattaacatt
     2221 aaaaagatgg cttacgcttt cggctaacgt tgtcgttggg cggccaatca atttgctaag
     2281 cgttttgctg ttatcaaaca gaccaccttt cgatgcgcca acgtcagaat ccgccagcat
     2341 atccgccagt ccgtcgggca gtccgacgct tttcagtgcc gcggcgaaat cggcttcgct
     2401 cagattttga taggtaacct gtttgccgct ctgtttggtc agctctgccg ctaactgtgt
     2461 caatgtccag gcactatcgc ccgccagttc gtaaaccttg ccttcgtgac cggcttcgct
     2521 aatcacgcgt gccgcagctg ccgcataatc tgcccgcgtt gctgaggcga ttttgccatc
     2581 gcccgccgca ccgataaata cgccgtgttc cagtgctgcc ggggcgctgg cgaggtagtt
     2641 ttcgctgtac cagccgttgc gcagcagggt gtaaacgatg ccagaatcag ccaacatttt
     2701 ctccgtctcg atgtgctcat cggcgaggcc gagcggggag gtatctgcat gtagcaggct
     2761 ggtataagcg ataaatttca caccagccgc ctttgcggca ttaataacat tacgatgctg
     2821 cggggcacgt tgacccactt cgctggaaga gatcagcagt agtttttcca ctccctgaag
     2881 tgcagatgtc agtgcggctt catcgccgta gtcagcctga cgcacggtaa tgccttgtgc
     2941 tgccagagcc tgggcttttg ccggattacg aacgatagcc actatttggc tgacaggaac
     3001 cgttttcttc aaggattcaa taacatagtg accaagttgg ccagtggcac cagtaatagc
     3061 gatcatggga agtctccatc gttttgtctt gtgtttaaat acgctaacac ctaaacttac
     3121 ttttagtaag tacgcacaaa aaggtaagta tgaaatgagt caggttagcc tgtcgcaaca
     3181 actgaaagag ggtaatctct ttgcggaaca gtgcccgtcg cgcgaggtgt tgaaacacgt
     3241 caccagccgt tggggggtgt tgattctggt ggcgctacgc gaaggtactc atcgctttag
     3301 cgacctgcgg cgcaaaatcg gcggggtgag cgaaaagatg cttgcgcagt cgttacaggc
     3361 gttagaacag gatggttttc ttaaccgtat cgcgtatccg gtggtgccgc cgcatgtgga
     3421 atatagcctc acgccgctgg gcgagcaggt gagcgaaaag gttgctgcac tggcggactg
     3481 gattgagttg aatttgcccg aggtgttggc ggtgcgggat gaacgtgcgg cataactgcc
     3541 ggatgcgctg agcttatctg gtctacggtg catggcttgt agggcggata agatgcgcca
     3601 gcatcgcatc cgccaataaa tgccggatgc ggttgatgat ttataggccg aaaaagaagt
     3661 gttttactta ctcaaatcca cctgataaat cgcaaacccg atatcatcgg tcgcgacttt
     3721 attcatcgga tattgccctt tctctttaat aaacgctgcg gctttatctg acggagaagt
     3781 ttcgaaacgg atatccagtt tcttatcgcc agctatcggt gctaaacgcc agttgttatc
     3841 tgccgccggg tgaatttccc ccgcacgttt cgactcatca gcaatccacg ctgccagcac
     3901 cgagcggttc tcatccggtg aagcaaaagc gatatggctg tcgcccgtac cggcaaattt
     3961 gccgccgtaa gcgcgatagt tattggtggc aacgaggaac atggcgttcg gatcaatcgg
     4021 cttgccatta aaggtcaggt tcttaatcct ttccgcattg gcattaatca tctggcactc
     4081 gccgtcataa cgggcaggct gggtaacatc aatctgataa ttcacaccat caatcacatc
     4141 aaagttatag gtgcggaaac catcccagtt gatgagtgat tgcggtttcg tgctgttagg
     4201 atcaatctgg ttaaactgtc cggcggagca ctccagccac tctttcacct ctttaccgct
     4261 ggctttcacc acaatcagcg tattggggta gagataaaga tcggcggcat tacggaaggt
     4321 caactggcct ttttccacct ccacatagct tgccgggtca tttttgcgac caccaacttt
     4381 aaacggtgcg gcagctgaaa gcaccggcag ttttgccaga tccggatcgc cctgaatgta
     4441 atgttcgaca tacgcttttt gcgcgttgtt cactacttgc acggtcggat cgtcctgcac
     4501 cagcgccaga tagctataca tattgtcggc agatttaccg attggcttgc tgacgaactg
     4561 gcgtgtggca tcgtgatcgg ctttgagtgt ttctaccagc ttgctgtctt ccgccgcgag
     4621 ggatttttta ttggcgatgt cgtaaatcgg ccgagcttcc gctttcgcct gcgtcacctg
     4681 ccatttaccg ctgtcattac tgagttgtaa gtcgaccacc ccaagatgat cgccccacat
     4741 gcccggcatt accgccggaa caccattcag cgtgcctttg gtgatatcag ccccttcgat
     4801 atcagcaaaa tctttacccg ggaaaacggc gtgagcatga ccaaacataa tggcgttaac
     4861 gcccggaatt tcactgaggt aataaactga gttttccgcc attactttat acggatcggc
     4921 agacagcccg gaatgcgcca gaacgacaac gacatcggca cctttctcgc gcatttcagg
     4981 cacgtatttg cgcacggttt cggtaatatc attcaccgtc acttttccgg ataaattagc
     5041 tttatcccag cccatgattt gcggcggcac gacgccaata tagccaatct tcagcgtctg
     5101 tttttttccg tctttatcga ccacttcggt atctttaatt aaatacggtg taaacattgg
     5161 ctgtttggtt ctggcgtcaa tgacgttggc atttacataa gggaatttcg ctcccgccag
     5221 cgcatttttc aggtaatcca gaccgtagtt aaattcatga ttgccgagtg taccgaccgt
     5281 ataatccagc gtattcagcg ccttataaac cggatgaaca tcacctgctt ttaatccttt
     5341 cgccgacatg taatcggcca gcggactccc ctgaatcaaa tcgccgttat cgaccagtac
     5401 gctgtttttc acttcattgc gggcatcgtt aatcaggctt gccgtacgta ccagtccgaa
     5461 tttttccgtg gcggtgtctt tgtaataatc gaaatccatc atgttgctat gcagatcagt
     5521 ggtttccatg atacgcagat cgaccgtcgc tgcattcaca ctggcggcaa tcagcgtggc
     5581 caggagcgtt gcgctaaact taatcatcag ggacatcctt ttatcatcgg gaatacgaaa
     5641 gaaaagggag aataaacgtc ttacttatag aacagtgaag aatgccacaa ttttacgctt
     5701 tgaaaatgat gacactatca cagttggcgc attcattaac gatagggtat aagtaaaaca
     5761 ataagttaac accgctcaca gagacgaggt ggagaaatgt tagatcaagt atgccagctt
     5821 gcacggaatg caggcgatgc cattatgcag gtctacgacg ggacgaaacc gatggacgtc
     5881 gtcagcaaag cggacaattc tccggtaacg gcagcggata ttgccgctca caccgttatc
     5941 atggacggtt tacgtacgct gacaccggat attccggtcc tttctgaaga agatcctccc
     6001 ggttgggaag tccgtcagca ctggcagcgt tactggctgg tagacccgct ggatggtact
     6061 aaagagttta ttaaacgcaa tggcgaattc accgttaaca ttgcgctcat tgaccatggc
     6121 aaaccgattt taggtgtggt gtatgcgccg gtaatgaacg taatgtacag cgcggcagaa
     6181 ggcaaggcct ggaaagaaga gtgcggtgtg cgcaagcaga ttcaggtccg cgatgcgcgc
     6241 ccgccgctgg tggttatcag ccgttcccat gcagatgcgg agctgaaaga gtatctgcaa
     6301 cagcttggcg aacatcagac cacgtccatc ggctcttcgc tgaaattctg cctggtggcg
     6361 gaaggacagg cgcagctgta cccgcgcttc ggaccaacga atatttggga caccgccgct
     6421 ggacatgctg tggccgcagc tgccggagcg cacgttcacg actggcaggg taaaccgctg
     6481 gattacactc cgcgcgagtc gttcctgaat cctgggttca gagtgtctat ttattaatta
     6541 atttatgcag caggtccatc acttgctgca cctcttcctg agtaagcgcc ccgtctttgg
     6601 cccattgcac gcgaccgtct ttatccagca ccactacagc ggaacttttt tcatccagct
     6661 gccaggcacc gcgtgcgacg ccattgctat caacaataaa ctgcgaccag ggataaagct
     6721 ttttattact ctccagactg ctgcgcacaa acatgccgga acccggaatt gcgtcgtcgg
     6781 tgttaacaat ggtggtggtc tggtaacgat cgtgcggtaa cttcgctgat ttaatcgctt
     6841 caatcagcgt cgcgtttttc tcttttgcag aggtgcgacc agcaatatgt tgcagtactc
     6901 gcacttttcc cactaactgc gcgctgttcc aggttttgta gctaaactga tctttatcaa
     6961 gcaccaactc gccacgatcg gtgatgccaa tcggcggcac tcgctgaccg gtttcgaact
     7021 gatgtgcgga agccatcatc ggcaacagca ggcaggtgag tgccagaatc ttgcgtaggg
     7081 tcatggtgtt tccttcttat gatatgcagg tgatccgacc acttgggtct gagttttaat
     7141 catatgtgct atttatcgaa tttcccgcaa gtgtgatgcc agtttgcggt caagcgcaca
     7201 aatcatatga aaaatgaatg cttatactga agaccgcgct tcggtaaaaa gataattctg
     7261 aataattgta acctttaggt aaaaaaagtt atacgcggtg gaaacattgc ccggatagtc
     7321 tatagtcact aggcattaaa atttgcgcct cataatagtt gggccgattg tggcaccgca
     7381 caggcgtaat actcagcagg agataacaat gaaaattttc caacggtaca acccacttca
     7441 ggtggcgaag tacgtaaaga tcctgttccg tggacggtta tacatcaagg acgttggcgc
     7501 ttttgaattt gataagggta agattcttat cccaaaagtg aaggataagc tgcatttgtc
     7561 agtgatgtcc gaagttaacc gtcaggttat gcgtctgcaa acagagatgg cttaaccaaa
     7621 gtgctatgca gtaataagac ggctcctgat tcaggagccg ttgatgtttc tgggggttac
     7681 gcgacgcttt cttctttatc tttagcgtca ggcagttttg gcgaaagggc ggtggccttg
     7741 ctgtcgatcc gggtcaccag cagctggtcg atgcggtagt tatcgatatc caccacttca
     7801 aatttgtagc cggcgaattt caccgaatcg gtgcgtttcg ggatcttacg cagcataaac
     7861 atcataaagc ctccgatggt ttcgtagttg cccgactgcg ggaactcgtc aatatccagc
     7921 acgcgcatga cgtcgtcaat tggggtgccg ccgtcaatca gccatgagtt ctcatcacgg
     7981 gcgacaatct gctcttccag cccctgaccg accagatcgc ccatcagcgt ggtcatcacg
     8041 tcgttgaggg tgatgatccc caccaccagc gcgtactcgt tcatgatcac cgcgaagtct
     8101 tcacctgcgg ttttaaaact ttccaacgcc tctgaaaggg ttaacgtatc cggcacaatc
     8161 agcgtgttgc gaatttgtac gccgctgttc agtgccaggc tttggttagc cagcacgcgg
     8221 ttcagcaggt ctttagaatc gacataaccg atgatgtggt caatatcttc attacagacg
     8281 aggaacttag agtgcggatg ttccgccacc ttattcttca ggctttgctc atcttcgtgg
     8341 agatcaaacc aaatcacgtt ttcacgcggt gtcattgaag acggaacggt acgggattcc
     8401 agctcaaaga cgttttcaat cagctcgtgt tcctgtttac gtaacacgcc cgccagcgca
     8461 ccggcttcca ctaccgcgta gatgtcatca gaagtgatgt catctttacg taccattggc
     8521 agtttgaaaa tacggaagat catgttcgcc aggccgttga agaaccacac cagcggggtg
     8581 caaacgtaca ggcagaagcg catcgggttg atgatacgca aagccaccgc ttctggcgca
     8641 atcataccga tgcgtttcgg ggttaaatcc gcaaacagga taaacatgcc agtcactaac
     8701 gagaaagaga gaataaagct cagttgctca gagagttctg ccgacatata gcgggagaac
     8761 aggctgtgaa aagctggaga aaatgccgca tcaccgacga taccgccgag aatcgcaact
     8821 gcgttcagac cgatttggac caccgtaaag aacatgccgg gattttcctg catattcaga
     8881 acgcgttggg catttatatt gccttcatca gccagcagtt taagtttgat tttgcgtgag
     8941 gcggcaagtg agatctcaga catcgagaag aacgcactta cagcgatcaa gcagagtatg
     9001 actaaaatac tgtttaacat atcttatccg acctttcagg ccggatcctc ggaaggggaa
     9061 gttgattaat ttgtgtgaaa tacacattga aagccgattc gaagaaagtg aaccggcatt
     9121 ttcagtggct agtatagcgt aaggtactgt aaagtcgcca gagggttaaa attcagactg
     9181 ctgataaatg caatactgcc tgatgcgcta tgccgatcag gcctacgcag tccctgcaat
     9241 atattgaatt tggacgattt tgtaggcctg gtaaggcatt cacgccgcat tcggcataaa
     9301 caaaacgcac tttatcaaca atctacattt gtacccgtaa cgctatgctt ccggtggcag
     9361 acagacgcca attccgccaa ttccacagta accatacggg tttttatgca gatattgctg
     9421 gtggtcatct tcggcataat aaaacggtgt ggcgttagcg atttccgtgg tgatgtgacg
     9481 atcgtcatcg gcggcaagca tcgccgcctg aaaacgttcc agactggcgc gagctgcggc
     9541 atcctgttct ggggtcagcg gataaatcgc tgaacgatac tgcgtgccgt ggtcattgcc
     9601 ctgacgcatt ccctgggcgg gatcgtgatt ctcccaaaat acctgtagca actgctcata
     9661 gctgatgaca gaaggatcgt aaacaatgcg taccgcttcg gcatgacccg tatcaccgga
     9721 gcacacttcc cgataagtcg gatttggcgt atagccgccg gtatagcctg cggcggtgct
     9781 gtaaacgccg ggtaactgcc agaacagacg ctccacaccc cagaaacaac ccatcgcaaa
     9841 aatggcaatc tccattccgt caggtacatt ggtcattgag tgaccgttga ccgcatgcag
     9901 cgtggctacg ggcatcgggg tgttacgtcc aggcagggca tcggcggggg aaaccagatg
     9961 ctttttatca aataaactca tggtgtcgct ctcccgaaaa tcggtcattg gggttaaggt
    10021 tgtaacaaga ggcgtatttg cacacaataa ccaccgtgaa taggtctaaa gtaaaacata
    10081 agaaatattt ggggtttagt ctgcttttta atccatatta ctggattttt gttaagccgt
    10141 ttaacggcgt tccaggggca ggaaaaaagg atattcagga gaaaatgtgc gctatatccg
    10201 acagttatgc tgtgtaagct tactctgctt aagcggatct gccgtcgccg cgaacgtccg
    10261 tctacaggtc gaggggttat cgggacagct ggaaaagaac gttcgtgcgc agctttctac
    10321 gattgaaagt gatgaagtga cgccagaccg tcgctttcgc gcacgcgtcg atgatgccat
    10381 ccgcgaaggt ctgaaagcgc tgggttatta ccagccgacc attgaatttg atctccgtcc
    10441 accgccaaag aaagggcggc aggtattgat cgccaaagtc acgccaggcg tgccggtgtt
    10501 aattggcggc accgatgtgg tattgcgcgg cggcgcgcgg accgataaag actatttgaa
    10561 attgctcgat actcgcccgg ctattggcac ggtactgaac cagggcgatt atgaaaattt
    10621 caaaaagtcc ttaaccagca ttgcgttgcg taaaggttat ttcgatagcg aatttaccaa
    10681 agcgcagctg ggcattgcgc tcggcctgca taaagccttc tgggatattg attataacag
    10741 tggcgaacgt taccgctttg ggcatgtgac ctttgaagga tcacaaatcc gcgatgaata
    10801 cctgcaaaat ctggtgccgt ttaaagaggg cgatgagtac gaatcgaaag atctggcgga
    10861 acttaaccgt cgactttctg ctaccggctg gtttaactcg gtcgtagtgg ctccacaatt
    10921 tgataaagcg cgcgaaacga aagtattacc cttgacgggc gtggtttcgc cgcgaactga
    10981 aaacaccatc gaaaccgggg tcggttactc tacggacgtg ggaccgcgcg tgaaagctac
    11041 gtggaaaaag ccgtggatga actcttatgg tcacagtctg accaccagta ccagtatttc
    11101 cgcgccggaa cagaccctcg acttcagcta taaaatgccg ctgctgaaga atccactgga
    11161 acaatattat ttggtgcagg gcggttttaa gcgcactgac ctgaacgata ccgaatctga
    11221 ctccactacg ctggtggctt ctcgctactg ggatctctcc agcggctggc agcgtgccat
    11281 taacctgcgc tggagtctcg accactttac tcagggtgaa attaccaaca ccacgatgct
    11341 gttttatcct ggggtgatga ttagccgcac gcgttctcgt ggtggcctga tgccaacctg
    11401 gggcgactcg caacgctact ctatcgacta ctccaacacg gcctggggct cagatgtcga
    11461 tttctccgtt ttccaggcgc agaacgtctg gatccgcaca ctgtacgatc gccatcgttt
    11521 cgttacacgc ggcacgctgg gctggattga aaccggtgat ttcgacaaag taccgccgga
    11581 tctgcgtttt ttcgccgggg gcgatcgcag tattcgcggc tacaaataca aatctatcgc
    11641 cccgaaatac gccaacggtg acctgaaagg ggcctcgaag ttgataaccg gatcgctgga
    11701 ataccagtac aacgtgaccg gaaaatggtg gggcgcggtg tttgtcgata gtggcgaagc
    11761 ggtaagcgat attcgccgca gcgactttaa aaccggtacc ggggtcggcg tgcgctggga
    11821 atcgccggtc gggccaatca aactcgattt tgccgtaccg gtcgcggata aagacgaaca
    11881 cgggttacag ttttacatcg gtctggggcc agaattatga gtttatggaa aaaaatcagc
    11941 ctcggcgtgg ttatcgttat cttactgttg ctgggatcgg tggcgtttct ggtgggcacc
    12001 accagcggcc tgcatcttgt atttaaagcg gcggatcgct gggtgccagg actggatatt
    12061 ggcaaggtca ccggcggctg gcgcgatctc accttgtctg acgttcgtta tgagcagcca
    12121 ggcgtggcgg taaaagcggg taatctgcat ctggcggtcg gtcttgagtg cctgtggaac
    12181 agtagcgttt gtattaatga cctggcgctg aaagacattc aggtcaacat cgacagtaaa
    12241 aaaatgcctc cttctgaaca ggttgaagaa gaggaagata gcggtccgct ggatctctcc
    12301 acgccgtatc ccatcaccct gacacgggtg gcgctggaca acgtcaacat caagattgat
    12361 gacaccacgg tgtcggtgat ggacttcacc tccggcctga actggcagga gaaaaccctg
    12421 accctgaaac cgacgtcgct gaaaggcctg ctgattgctc tgccgaaagt ggcggaagtg
    12481 gcgcaggaag aagtggtcga accgaaaatt gaaaatccgc agccggagga aaagccgctc
    12541 ggcgaaacgc tgaaagatct cttttctcgc ccggtattgc cggaaatgac cgacttgcat
    12601 ttgccgctta acctgaacat tgaagagttt aaaggcgagc agttacgcgt gacgggcgac
    12661 acggacatca ccgtgcgcac catgctgctg aaagtgagca gcattgacgg caatactaaa
    12721 ctggacgccc tggatatcga ttccagtcaa gggatcgtca acgccagcgg cacggcgcag
    12781 ctgtcagaca actggccggt ggatatcacc ctcaacagca cactgaacgt ggagccgttg
    12841 aaaggtgaaa aagtgaagct aaaagtgggc ggcgcgctgc gcgaacagct ggagattggc
    12901 gttaatcttt ccggtccggt ggatatggat ttacgcgccc agacgcgact ggcggaagcc
    12961 ggattgccac tcaacgtgga agtgaacagc aaacagcttt actggccgtt caccggtgag
    13021 aagcagtatc aggcggatga tctgaaactg aaacttaccg gcaaaatgac cgattacacg
    13081 ctctctatgc gtacggcagt gaagggactg gagatcccgc cagccaccat tacccttgat
    13141 gccaaaggta atgaacagca ggtcaatctc gacaaactca ccgtcgcggc gctggaaggg
    13201 aaaactgaac tcaaggcgtt gctcgactgg cagcaggcca ttagttggcg cggtgagcta
    13261 acgcttaacg gcattaacac cgccaaagag atcccagagt ggccgtcgaa actcaatggc
    13321 ttgattaaaa cccgcggtag cctgtacggc ggcacctggc agatggaggt gccagaactg
    13381 aagctgaccg gtaacgtcaa acagaacaaa gtgaacgttg acggcacgct gaaaggcaac
    13441 agttatatgc agtggatgat cccagggctt catctggaac tggggccaaa cagtgccgaa
    13501 gtgaaaggcg agctgggggt aaaagatctc aatcttgatg ccaccatcaa cgcgccgggg
    13561 ctggataacg cgctgccggg gcttggcggt acagcgaaag ggctggtgaa agttcgcggc
    13621 acggtggaag cgccacaact actggcggac atcaccgcgc gcggcctgcg ctggcaggag
    13681 ctaaccgtgg cgcaggttcg cgtggaaggc gatatcaaat ccaccgttca gattgcgggt
    13741 aaactcgacg tgcgcgttga gcaaatttca cagccggatg taaatatcaa cctcgtcacc
    13801 ctgaatgcca aaggcagcga aaaacagcac gagttacagt tgcggattca gggcgagccg
    13861 gtttccgggc agcttaatct ggcaggaagt tttgatcgca aagaagaacg ctggaaggga
    13921 actcttagca atacccgctt ccagacgccg gtcggcccgt ggtcgctgac ccgcgatatt
    13981 gcgctggatt accgcaataa ggagcaaaaa atcagcatcg ggccacactg ctggcttaac
    14041 ccgaatgcgg aactgtgcgt gccgcaaact atcgatgcgg gggccgaagg gcgtgcggtg
    14101 gtgaatctca accgcttcga cctcgccatg ctgaaaccgt ttatgccaga aaccactcag
    14161 gccagcggta tcttcacggg taaagcggat gttgcctggg acaccacgaa agaggggctg
    14221 ccgcagggca gtatcaccct ttcggggcgt aacgtgcagg taacgcaaac cgtcaacgat
    14281 gcggcgctgc ccgtggcgtt tcagacactg aatctgacgg cggaattgcg taacaaccgt
    14341 gccgaattgg gctggaccat ccgcctgacc aataacggcc agtttgatgg acaggtgcag
    14401 gtgaccgatc cgcaaggccg ccgtaatctt ggtggcaacg tcaatatccg taacttcaac
    14461 cttgcgatga taaaccccat ctttaaccgt ggggaaaaag cagcggggat ggtgagtgcc
    14521 aacttgcgtc tgggtggtga tgtgcaaagc ccgcagttgt ttggtcagct tcaggttacg
    14581 ggtgtggata tcgacggcaa ctttatgccg tttgatatgc aaccgagcca gcttgcggtc
    14641 aactttaatg gtatgcgctc gacgcttgcc ggtacagtac ggacccagca gggtgaaatc
    14701 tacctgaacg gtgatgccga ctggagccaa attgaaaact ggcgggcgcg ggtaacggcg
    14761 aaaggcagta aagtgcggat caccgtgccg ccgatggtac gaatggatgt atcgccagat
    14821 gttgtcttcg aggctacacc aaacctgttt accctcgatg gtcgcgtgga tgtcccgtgg
    14881 gcgcgcatcg tggtgcacga tctgccggaa agcgcagtag gcgtctccag cgatgtggtg
    14941 atgcttaacg ataacctgca acctgaagag ccgaaaacgg cgtcgattcc gattaacagt
    15001 aacctgattg tccacgttgg caacaatgtg cgcattgacg cctttggcct gaaagcgcgg
    15061 ctgacgggcg atcttaacgt cgttcaggac aaacaagggc tgggcctgaa cgggcagatc
    15121 aacatccctg aagggcgctt ccatgcctat ggtcaggatc tgattgtgcg taaaggtgag
    15181 ttactgttct ctggtccgcc ggatcaaccg tatcttaata ttgaagctat tcgtaacccg
    15241 gatgctacag aagacgacgt aatcgccgga gttcgcgtca ctggtctggc ggacgaaccg
    15301 aaagcggaga tcttctctga cccggcgatg tcgcaacaag ctgcattgtc ttatttgcta
    15361 cgtggacaag ggctggagag cgatcagagc gacagtgcgg caatgacctc gatgctgatt
    15421 ggtctggggg ttgcgcaaag tggccagatt gtgggtaaaa tcggcgagac gtttggcgta
    15481 agcaatttag cgctcgacac ccagggagta ggcgactcct cccaggtagt ggtcagcggc
    15541 tatgtattgc caggtctgca agtgaaatat ggcgtgggta tatttgactc tatagcaaca
    15601 ctcacgttac gttatcgcct gatgcctaag ctatatctgg aagccgtgtc tggtgtagac
    15661 caggcactgg atttgctcta tcagttcgag ttttagcaat gcgaatattt gtctacggca
    15721 gtttacgcca caaacaaggc aacagtcact ggatgaccaa tgcccagtta ctgggcgatt
    15781 tcagtatcga taactaccag ttgtatagcc tgggccacta tccaggcgca gttccgggga
    15841 acggaacagt acacggtgaa gtttatcgta ttgacaacgc cacgctggcc gaacttgatg
    15901 ccttgcgcac caggggcggt gaatacgcgc gccagttgat tcagacgccg tacgggagtg
    15961 catggatgta cgtttatcaa cgacccgtcg atggattaaa gctaattgaa agcggcgact
    16021 ggttagacag ggataagtaa ccatatgcat acgccacctt cgggtggcgt tgttttttgc
    16081 gagacgactc gcattctgtt ttgtaattcc ctcacccttt gcttttctct ccgagccgct
    16141 ttccatatct gttaacgcat aaaaaactct gctggcgttc acaaatgcgc aggggtaaaa
    16201 cgtttcctgt agcaccgtga gttatacttt gtataactta aggaggtgca gatgcgtatt
    16261 accataaaaa gatgggggaa cagtgcaggt atggtcattc ccaatatcgt aatgaaagaa
    16321 cttaacttac agccggggca gagcgtggaa gcgcaggtga gcaacaacca actgattctg
    16381 acacccatct ccaggcgcta ctcgcttgat gaactgctgg cacagtgtga catgaacgcc
    16441 gcggaactta gcgagcagga tgtctggggt aaatccaccc ctgcgggtga cgaaatatgg
    16501 taaagaaaag tgaatttgaa cggggagaca ttgtgctggt tggctttgat ccagcaagcg
    16561 gccatgaaca gcaaggtgct ggtcgacctg cgcttgtgct ctccgttcaa gcctttaatc
    16621 aactgggaat gacgctggtg gcccccatta cgcagggcgg aaattttgcc cgttatgccg
    16681 gatttagcgt tcctttacat tgcgaagaag gcgatgtgca cggcgtggtg ctggtgaatc
    16741 aggtgcggat gatggatcta cgcgcccggc tggcaaagcg tattggtctg gctgcggatg
    16801 aggtggtgga agaggcgtta ttacgcttgc aggcggtggt ggaataaggt gtgtttattt
    16861 atcgcgggca taaaaaaacc cttactaacc gaagctcggc gttcagggtt attgcgctag
    16921 aagaacttat ttattctttg cgcgctcgaa ggaagcaacg atttcagctt tagcggcttc
    16981 tgcgttttcc caaccttcaa ctttcaccca cttgcctttt tcgaggtctt tgtagtgctc
    17041 gaagaagtga gcgatctgcg ctttcagcag ctctggcaga tcgttaacgt ctttaatgtg
    17101 atcgtattct ttgctcagct tgctgtgcgg aaccgcaacc agtttcgcat cttcaccggc
    17161 ttcgtcggtc attttcagaa cgccaaccgg acggcaacgg atcacagaac ccggctgcag
    17221 cgggtacgga gtcgggacca gtacgtcaac cgggtcaccg tccagagaca gggtgtggtt
    17281 gatgtaaccg tagttgcacg gatagaacat cgcggtggac atgaagcggt caacgaacag
    17341 tgcgccgctc tctttgtcga tttcgtattt gatcggatct gcgttagccg ggatctcaat
    17401 aacaacgtag atgtcttccg gcagatcttt acccgcaggg acgttgagta agctcatgtc
    17461 tgtttccttt aaaaatatgt ggcaaacaag tgccgagtat tatagccaac tcgcgccgaa
    17521 tgtctttgct tgttttcgtc ttcattttcc tttttcgcca gttttaagac ggtatacaga
    17581 gcaggaaaat ccataaccgt agccgcattt ttcatagtga gatgaaagcg attacaaact
    17641 tgtgattaac gttttattta cttttttgaa gtgtgatgta acgcaatccg ttacataacg
    17701 aattgtctat agttttttcg cgaacatctt ttcaccaata ataattaccc cgacgaggac
    17761 aaccctatgt ggaaacgctt acttgtagtc tctgcagtct cggcagccat gtcgtctatg
    17821 gcgttggccg ctccattaac cgtaggattt tcgcaggtcg gatcggaatc cggctggcgc
    17881 gctgcagaaa ccaatgtggc gaaaagtgag gccgaaaaac gcggaattac gttgaaaatt
    17941 gccgatggtc agcaaaagca ggaaaaccag attaaagcgg tacgttcctt cgttgcacaa
    18001 ggggtggatg cgatctttat cgctccagtg gttgcgacgg gttgggaacc ggtattaaaa
    18061 gaggcgaaag atgccgaaat cccggtcttc ttgcttgacc gttccatcga tgtgaaagac
    18121 aaatctctct atatgaccac cgtcactgcc gacaacatcc tcgaaggcaa gttgattggt
    18181 gactggctgg taaaagaagt gaatggcaaa ccatgcaacg tggtggaact gcagggcacc
    18241 gttggggcca gcgtcgccat tgaccgtaag aaaggctttg ccgaagccat taagaatgcg
    18301 ccaaatatca aaatcatccg ctcgcagtca ggtgacttca cccgcagtaa aggcaaagaa
    18361 gttatggaga gctttatcaa agcggaaaac aacggcaaaa acatctgcat ggtttacgcc
    18421 cataacgatg acatggtgat tggtgcaatt caggcaatta aagaagcggg cctgaaaccg
    18481 ggcaaagata tcctcacggg ttccattgac ggcgtaccgg atatctataa agcgatgatt
    18541 gatggcgaag cgaacgccag cgttgaactg acgccgaaca tggcaggccc cgcttttgac
    18601 gcgctggaga aatacaaaaa agacggcacc atgcctgaaa agctgaccct gaccaaatcc
    18661 accctttatc tgcctgatac cgcaaaagaa gagttagaga agaagaaaaa tatggggtat
    18721 tgagggttgc tatgcctgat gccgattcgt aggccggata aggcgctcgc gccgcatccg
    18781 gcgatggtgc actgaagcct gatgcgacgc ttaccgcgtc ttatcatgcc tactgggagc
    18841 acgctttaca ccgggggaaa ccatgacgac cgaccaacac caggagatcc tccgcaccga
    18901 aggattaagt aaatttttcc ccggcgtcaa agcgttagac aacgttgatt tcagcctgcg
    18961 ccgtggcgaa atcatggcgc tgcttggtga aaacggggcg ggaaaatcaa cgctaatcaa
    19021 agcattaact ggtgtatacc acgccgatcg cggcaccatc tggctggaag gccaggctat
    19081 ctcaccgaaa aataccgccc atgcacaaca actcggtatc ggcaccgtct atcaggaagt
    19141 caacctgcta cccaatatgt cggttgctga taatctattt ataggccgcg aacccaaacg
    19201 tttcggcctt ctacgccgta aagagatgga aaagcgcgcc accgaactga tggcatctta
    19261 cggtttctcc ctcgacgtgc gcgaaccgct taaccgtttt tcggtggcga tgcagcaaat
    19321 cgtcgccatt tgccgggcta tcgatctctc cgccaaagtg ctgatcctcg atgaacccac
    19381 cgccagcctc gacacccagg aagtggagct attatttggc ctgatgcgtc agttgcgcga
    19441 tcgcggcgtc agcctgatct ttgtcactca ctttctcgat caggtctatc aggtcagcga
    19501 tcggatcacc gtcttacgca acggcagttt cgtaggctgt cgggaaacgc gcgagctacc
    19561 gcagatcgaa ctggtaaaaa tgatgctggg gcgcgagctg gatacccacg cgctacagcg
    19621 tgccgggcga acattgttga gcgacaaacc cgttgccgcg ttcaaaaatt acggcaaaaa
    19681 aggaacgata gcaccgtttg atctcgaagt gcgccccggc gagatcgtcg gtctggctgg
    19741 cttgctggga tccggacgta ccgaaaccgc cgaagtgatc ttcggtatca aacctgctga
    19801 cagcggcacg gcgttgatca aaggcaaacc gcaaaccctg cgatcgccac atcaggcttc
    19861 agtactgggc atcggattct gcccggaaga caggaaaacc gatggcatca tcgctgccgc
    19921 ctcggtgcgg gaaaatatca tcctcgctct ccaggcccag cgcggctggc tacgacccat
    19981 ttcccgcaaa gagcagcaag agattgccga acgctttatc cgccagcttg gcattcgcac
    20041 accttcaact gaacaaccga ttgaatttct ctctggcggc aatcagcaaa aagtgttgct
    20101 ttcacgttgg ctactgacac gaccgcaatt tctgatcctc gacgagccaa cccgcggcat
    20161 tgatgttggt gcccacgccg agatcatccg cctgattgaa acgctgtgtg ctgacggtct
    20221 ggcgctgttg gtgatctcct ccgaactgga agagctggtg ggctatgccg atcgggtgat
    20281 tatcatgcgc gatcgcaaac aggtggcgga gatcccgctg gcagagcttt ccgttccggc
    20341 gatcatgaac gccattgcgg cgtaaggaga acagtgtgat gcctcaatct ctcccggaca
    20401 ctacgccgcc gaaaaggcgc tttcgctggc caacgggaat gccgcagctg gcggcgctat
    20461 tgctggtgct gctggtcgat agcctggtag ccccgcattt ctggcaggtg gtgcttcagg
    20521 atgggcgttt gttcggtagc cctatcgaca ttcttaaccg tgcagctccc gttgcgttac
    20581 tggcgattgg tatgacgctg gtgatcgcca ccggtgggat tgatctctcc gtaggggcgg
    20641 tgatggctat cgccggagcc acaacggctg cgatgacggt cgcgggattc agcctgccga
    20701 ttgttttgtt aagcgccctg ggcaccggca tcctggcggg attgtggaac ggcatactgg
    20761 tagcgatcct caaaattcag ccgtttgtcg ccaccctgat cctgatggtc gccgggcgcg
    20821 gcgtggcgca actgatcacc gccgggcaga tcgtcacgtt taactcgccg gatctctcat
    20881 ggtttggcag cggatcgctg ttgttcctgc caacgccggt cattatcgcg gtgctgacgc
    20941 ttctcctgtt ctggctgttg acccgcaaaa cggcgctggg gatgtttatc gaagccgttg
    21001 gtatcaacat tcgggcggca aaaaatgccg gggtaaacac gcggatcatc gtcatgctca
    21061 cctacgtgtt gagcgggctg tgtgcggcga ttgcgggcat tatcgtggcg gcggatattc
    21121 gcggtgccga tgccaataac gccgggttat ggctggagct ggacgccatt cttgcggtgg
    21181 tgattggcgg cggatcgctg atgggcgggc gctttaacct gctgctctca gtcgtggggg
    21241 cgctgattat tcaggggatg aacaccggaa ttttactttc tggctttccg ccggagatga
    21301 accaggtcgt aaaagcggtg gtggtgcttt gtgtgctgat tgttcagtcg caacgcttta
    21361 tcagtctgat taaaggagta cgtagccatg ataaaacgta acttgccgct gatgatcacc
    21421 atcggcgtct ttgtgctggg ctatctctac tgcctgaccc agtttcccgg ttttgcttcc
    21481 acaagagtga tctgcaatat cctgaccgat aacgcctttc ttgggataat cgccgttggc
    21541 atgacctttg tgatcctctc tggcggtatc gatctctccg tcggttcggt gatcgccttt
    21601 actggcgtgt ttctggcaaa agtgattggc gatttcggcc tctcgccgct gctggcgttt
    21661 ccgctagtgc tggtgatggg ctgtgccttt ggcgcattta tggggctttt gatcgacgcc
    21721 ctgaagatcc cggcatttat cattaccctg gcaggaatgt tctttttgcg cggcgtcagc
    21781 tatctcgttt cagaagagtc gatcccgata aaccatccca tttatgacac gctctcaagc
    21841 cttgcgtgga aaatccctgg cggcggtcgc ttaagtgcga tgggactgct gatgctggcg
    21901 gtggtggtta tcggcatatt cctcgcgcat cgtacccgtt ttggtaatca ggtatacgcc
    21961 attggcggca acgcaacgtc ggcaaacctg atggggattt ctacccgcag caccactatt
    22021 cgcatttata tgctctccac cggactggca acgctggcgg ggattgtctt ctcgatttat
    22081 acccaggccg gatatgcgct ggcgggcgta ggtgtggaac tggatgctat cgcctcagtg
    22141 gtaattggcg gtacgctttt gagcggtggc gttggaacgg tattagggac gctttttggc
    22201 gtggcgattc aggggctaat tcagacttat atcaactttg acggtacgct gagttcctgg
    22261 tggacgaaaa tcgccatcgg cattttgctg ttcattttta ttgccttaca gcgtggatta
    22321 acggtgctgt gggagaatcg tcagagttcg ccagtgacaa gagtcaatat tgcgcagcga
    22381 taaaaacgcc tctccgtgtg gagaggcgca ggagattacg cgtccgggaa ttcacggata
    22441 aagcgttcga catcttcaac catatggtcg ttgccgacaa agaacgaacg gcgctggtgc
    22501 agagtttccg ggatgatatc cagaatacgc tctttgccat cgctcgcttt accgcccgct
    22561 tgttccgcca ggaatgccat cgggttgcac tcatacagca aacgcagttt gccgtccggg
    22621 tggctggcgg tgcttgggta gagataaata ccgcctttca gcaggttacg gtggaaatcc
    22681 gcgaccagtg aaccgatata acgtgaggta tacgggcggt tggtggattt atcttcttcc
    22741 tggcagaatt taatgtactt cttcaccccg ttcggaaact taatgtagtt tccttcgttg
    22801 atggagtagg ttttgccttt ctccgggaag cgcatccgtt cctggcacag gcagaaaacg
    22861 cccagcgaag gatcgtaagt aaaggcgtga acaccgcatc cggtggtgta aaccagcatg
    22921 gtagaggagc cgtataccac gtaacctgcc gcaacctgtt tgttaccagg ctggaggaaa
    22981 tcttcttccg ttaccggcgt gccaacaggc gtaacgcggc ggtagatgga gaaaatggta
    23041 ccgacagaga cgttaacatc gatgttggac gagccatcca gggggtccat cagcaccacg
    23101 tattttgcgt gttcacagcc ttcaaagacg acaatctcat cttcttcttc agaggcaatg
    23161 cccgcaacga tatcgcgtgc tttcagtgcg gctttcagtt tttcattagc gaacaagtcg
    23221 agtttctgct gaacctcgcc ctgcacgttc tcagcaccgc tggcacccag gatatcaacc
    23281 agtcctgctt tgttgatatc gcgatggata atcttggcgc ccagttttat tgccgacagc
    23341 aaagcagtga gctcaccggt agcatgagaa aactcgtgct gcttttcgac aataaattca
    23401 cctaacgttt tcataaaact ttccctgcaa tgtttatgga gtaaagcgac cgcaacaatc
    23461 ttaacaaata atctcaatgt tgcgctcagg tgaatcgcgc cagcaaattg cggattatcc
    23521 tgaaatgcgt ttctcacttg cccgacatat gcgtaaaatg agcggcagat taaaaaagga
    23581 tagtgacgta tgcgcattca tattttagga atttgtggca catttatggg cggtctggcg
    23641 atgctggcgc gccagttagg ccatgaagta acgggttcgg acgccaatgt gtatccgccg
    23701 atgagcacct tacttgagaa gcaaggcatt gagctgattc agggttacga tgccagccag
    23761 ctcgatccgc agccggatct ggtgattatt ggcaacgcca tgacccgtgg aaatccgtgt
    23821 gtggaagcgg tactggaaaa aaacatccct tatatgtcag gtccacagtg gctgcacgat
    23881 tttgtgctgc gcgaccgctg ggtgctggcc gttgccggta cacacggcaa aactaccacc
    23941 gcgggaatgg cgacctggat tctggaacag tgcggttaca aaccgggatt tgtgatcggc
    24001 ggtgtgccgg ggaactttga ggtttcggcg cgtctgggcg aaagcgactt ctttgttatc
    24061 gaagcggatg agtatgactg cgccttcttc gacaaacgct ctaaatttgt ccattactgc
    24121 ccgcgtacgc tgatcctcaa caaccttgag ttcgatcacg ccgatatctt tgacgacctg
    24181 aaagcgatcc agaaacagtt ccaccatctg gtgcgtatcg ttccggggca gggccgtatt
    24241 atctggccgg aaaatgacat caacctgaaa cagaccatgg cgatgggctg ctggagcgag
    24301 caggagctgg tgggtgagca gggtcactgg caggcgaaaa agctgaccac tgacgcttct
    24361 gaatgggaag tcttgctgga tggcgaaaaa gtgggcgaag tgaaatggtc gctggtgggc
    24421 gaacataata tgcacaatgg cctgatggcg attgcggcgg ctcgccatgt tggtgtagcg
    24481 cctgcagatg ccgctaacgc gctgggttcg tttattaacg ctcgtcgccg tctggagttg
    24541 cgtggtgaag cgaatggcgt gacggtatat gacgattttg cccatcaccc gacggcaatt
    24601 ctggcaacgc ttgcggcgct gcgtggcaaa gttggtggta cggcgcgcat tattgctgtg
    24661 ctggagccgc gctcgaatac catgaaaatg gggatctgca aagacgatct ggcaccttca
    24721 ttaggtcgtg ccgatgaagt cttcctgctg caaccggcgc atattccgtg gcaggtggca
    24781 gaagtggcag aagcctgcgt tcagcctgca cactggagtg gcgatgtgga tacgctggca
    24841 gatatggtgg tgaaaaccgc tcagcctggc gaccatattc tggtgatgag caacggcggt
    24901 tttggtggga tccatcagaa actgctggat ggtctggcga agaaggcgga agctgcgcag
    24961 taattcggcc tcagcctgat atagtgtgcg taggccggat aaggcgttca cgccgcatcc
    25021 ggcatttgag catagtgcct gatgcgacgc ttgatgcgtc ttatcaggcc tacaggtgca
    25081 ccgcatccgg caaaccatac tcacatcaac aacgaaaatt acccttcatt ctccgccagt
    25141 tcgcgcagat actggaaaat ctggcgtgcg gatttcggcg gcttattccc ttctttctct
    25201 ttcttcgcgt tgcggatcag agtacgcagc tgctgacgat cggcatcgtg ccacagattt
    25261 aatacctcag cgatggcgtc atcaccttga tcgatcagac gatcgcgcag gttttccagt
    25321 ttatggaaca gcaccacctg ctggttgtga cggtttttca gcttatccag cgcctgacgg
    25381 atcggttcta catcgcgctg gcgcagcatc ttaccgatga gctgcaactg gcggcggcga
    25441 ccttccatct taatacgctg ggcgagctca atagccgcgc gcagatccgc atctaacggg
    25501 atcttatcca gcgcgttttt ccccagatca acaatttccg cgccaaggcg ttttagctcc
    25561 tcggcatcac gtttaatttc acttttactg acccagataa tttcatcgtc ttcgtcttcg
    25621 atgtcatcac cgggaacgtc gtcgagccag tcttcgggct gcttagtcat ctcaggctcc
    25681 ttaaaaaaag aggctaatgt taccagttaa gatgcgcact gaaaaacggt tctctgttag
    25741 acttcagaga aactctctac attatggcac ttgcaatgaa agtaatctct caagttgaag
    25801 cgcagcgcaa gattctggaa gaagcagttt ccactgcgct ggagttggcc tcaggcaaat
    25861 cggacggtgc ggaagttgcc gtcagcaaga ccaccggcat tagcgtaagc acgcgttatg
    25921 gtgaagtgga gaatgttgaa ttcaatagcg atggcgcgct ggggatcact gtttatcacc
    25981 agaaccgcaa aggtagcgca tcatccaccg atttaagccc gcaggccatt gcccgtactg
    26041 tacaggcggc gctggatatt gcccgttata cctcgccaga tccctgtgcc ggcgtggcag
    26101 acaaagagct gctggccttt gacgcaccag atctcgactt gttccaccct gcggaagttt
    26161 ccccggatga agccattgaa ctggcggccc gcgcagaaca ggcggcattg caggcggaca
    26221 aacgcattac caataccgaa ggtggcagct ttaacagcca ctacggtgtc aaagtttttg
    26281 gcaacagcca cggcatgttg cagggttact gctcaacgcg tcattcgctc tccagctgtg
    26341 taattgccga agaaaatggc gatatggagc gtgattacgc ctacaccatt ggccgcgcga
    26401 tgagcgatct gcaaacgcca gagtgggtcg gggccgactg tgctcgccgt actttatcgc
    26461 gcctgtcacc gcgtaaactc tccaccatga aagcgccagt catttttgcc aatgaagtgg
    26521 caaccgggct ttttggccat ctggtggggg cgatagcggg tggctcggtt taccgtaaat
    26581 ctacctttct gctggattcg ctgggtaaac aaattctgcc ggactggctg accattgaag
    26641 agcatccgca tctgctgaaa gggctggcgt cgacgccatt cgacagcgaa ggtgtgcgca
    26701 ccgagcgtcg cgatattatt aaagatggca tcctgactca gtggctgctg accagctact
    26761 cggcgcggaa actggggctg aaaagcaccg gacatgcggg cggtattcac aactggcgga
    26821 ttgccggaca agggctaagc ttcgaacaaa tgctcaaaga gatgggcacc gggctggtgg
    26881 tgacggagct gatgggccag ggcgtgagtg ccattaccgg tgattattcc cgtggtgcag
    26941 cgggcttctg ggtagagaat ggcgaaattc agtatcccgt tagtgaaatc accatcgcag
    27001 gtaatttaaa agatatgtgg cgcaatattg tcaccgtcgg taacgatatt gaaacacgca
    27061 gtaatataca gtgtggttct gtgctgttgc cggagatgaa aatcgccggg cagtaaaaaa
    27121 ctggcgcgac ctgccgcgct ataaactaaa aaattacaca aaatcattcg cactgcatcg
    27181 agacggaaag tgaatgaggg ccggggagcg tactcgcagt acgtgacccg ggcgaatgag
    27241 cgtagccgtc gatgaggcag tgtgaaggat gaagtgtaaa taaaaaagga agtgagcaat
    27301 gcgtaaaagc ctgttagcta ttcttgcagt ctcctcgttg gtattcagtt ctgcgtcgtt
    27361 tgccgctgat cttgaaga
//
LOCUS       DAZGLJ010000042        26925 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000042 DAZGLJ010000000
VERSION     DAZGLJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.042"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..567
                     /locus_tag="ACHK49_001595"
     CDS             <1..567
                     /locus_tag="ACHK49_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO9262264.1"
                     /translation="IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKE
                     TQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESE
                     VYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHP
                     IAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            750..944
                     /gene="bfd"
                     /locus_tag="ACHK49_001596"
     CDS             750..944
                     /gene="bfd"
                     /locus_tag="ACHK49_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO9262265.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            1017..1493
                     /gene="bfr"
                     /locus_tag="ACHK49_001597"
     CDS             1017..1493
                     /gene="bfr"
                     /locus_tag="ACHK49_001597"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO9262266.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1490..1957)
                     /locus_tag="ACHK49_001598"
     CDS             complement(1490..1957)
                     /locus_tag="ACHK49_001598"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO9262267.1"
                     /translation="MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            2336..2647
                     /gene="rpsJ"
                     /locus_tag="ACHK49_001599"
     CDS             2336..2647
                     /gene="rpsJ"
                     /locus_tag="ACHK49_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO9262268.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2680..3309
                     /gene="rplC"
                     /locus_tag="ACHK49_001600"
     CDS             2680..3309
                     /gene="rplC"
                     /locus_tag="ACHK49_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO9262269.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3320..3925
                     /gene="rplD"
                     /locus_tag="ACHK49_001601"
     CDS             3320..3925
                     /gene="rplD"
                     /locus_tag="ACHK49_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO9262270.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3922..4224
                     /gene="rplW"
                     /locus_tag="ACHK49_001602"
     CDS             3922..4224
                     /gene="rplW"
                     /locus_tag="ACHK49_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO9262271.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4242..5063
                     /gene="rplB"
                     /locus_tag="ACHK49_001603"
     CDS             4242..5063
                     /gene="rplB"
                     /locus_tag="ACHK49_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO9262272.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            5080..5358
                     /gene="rpsS"
                     /locus_tag="ACHK49_001604"
     CDS             5080..5358
                     /gene="rpsS"
                     /locus_tag="ACHK49_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO9262273.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5373..5705
                     /gene="rplV"
                     /locus_tag="ACHK49_001605"
     CDS             5373..5705
                     /gene="rplV"
                     /locus_tag="ACHK49_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO9262274.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5723..6424
                     /gene="rpsC"
                     /locus_tag="ACHK49_001606"
     CDS             5723..6424
                     /gene="rpsC"
                     /locus_tag="ACHK49_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO9262275.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6437..6847
                     /gene="rplP"
                     /locus_tag="ACHK49_001607"
     CDS             6437..6847
                     /gene="rplP"
                     /locus_tag="ACHK49_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO9262276.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6847..7038
                     /gene="rpmC"
                     /locus_tag="ACHK49_001608"
     CDS             6847..7038
                     /gene="rpmC"
                     /locus_tag="ACHK49_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO9262277.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            7038..7292
                     /gene="rpsQ"
                     /locus_tag="ACHK49_001609"
     CDS             7038..7292
                     /gene="rpsQ"
                     /locus_tag="ACHK49_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO9262278.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7457..7828
                     /gene="rplN"
                     /locus_tag="ACHK49_001610"
     CDS             7457..7828
                     /gene="rplN"
                     /locus_tag="ACHK49_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO9262279.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7839..8153
                     /gene="rplX"
                     /locus_tag="ACHK49_001611"
     CDS             7839..8153
                     /gene="rplX"
                     /locus_tag="ACHK49_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO9262280.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8168..8707
                     /gene="rplE"
                     /locus_tag="ACHK49_001612"
     CDS             8168..8707
                     /gene="rplE"
                     /locus_tag="ACHK49_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO9262281.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8722..9027
                     /gene="rpsN"
                     /locus_tag="ACHK49_001613"
     CDS             8722..9027
                     /gene="rpsN"
                     /locus_tag="ACHK49_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO9262282.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            9061..9453
                     /gene="rpsH"
                     /locus_tag="ACHK49_001614"
     CDS             9061..9453
                     /gene="rpsH"
                     /locus_tag="ACHK49_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO9262283.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9466..9999
                     /gene="rplF"
                     /locus_tag="ACHK49_001615"
     CDS             9466..9999
                     /gene="rplF"
                     /locus_tag="ACHK49_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO9262284.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            10009..10362
                     /gene="rplR"
                     /locus_tag="ACHK49_001616"
     CDS             10009..10362
                     /gene="rplR"
                     /locus_tag="ACHK49_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO9262285.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10377..10880
                     /gene="rpsE"
                     /locus_tag="ACHK49_001617"
     CDS             10377..10880
                     /gene="rpsE"
                     /locus_tag="ACHK49_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO9262286.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10884..11063
                     /gene="rpmD"
                     /locus_tag="ACHK49_001618"
     CDS             10884..11063
                     /gene="rpmD"
                     /locus_tag="ACHK49_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO9262287.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            11067..11501
                     /gene="rplO"
                     /locus_tag="ACHK49_001619"
     CDS             11067..11501
                     /gene="rplO"
                     /locus_tag="ACHK49_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO9262288.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11509..12840
                     /gene="secY"
                     /locus_tag="ACHK49_001620"
     CDS             11509..12840
                     /gene="secY"
                     /locus_tag="ACHK49_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO9262289.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12872..12988
                     /gene="rpmJ"
                     /locus_tag="ACHK49_001621"
     CDS             12872..12988
                     /gene="rpmJ"
                     /locus_tag="ACHK49_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO9262290.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13135..13491
                     /gene="rpsM"
                     /locus_tag="ACHK49_001622"
     CDS             13135..13491
                     /gene="rpsM"
                     /locus_tag="ACHK49_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO9262291.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13508..13897
                     /gene="rpsK"
                     /locus_tag="ACHK49_001623"
     CDS             13508..13897
                     /gene="rpsK"
                     /locus_tag="ACHK49_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO9262292.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13931..14551
                     /gene="rpsD"
                     /locus_tag="ACHK49_001624"
     CDS             13931..14551
                     /gene="rpsD"
                     /locus_tag="ACHK49_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO9262293.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14577..15566
                     /gene="rpoA"
                     /locus_tag="ACHK49_001625"
     CDS             14577..15566
                     /gene="rpoA"
                     /locus_tag="ACHK49_001625"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO9262294.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15607..15990
                     /gene="rplQ"
                     /locus_tag="ACHK49_001626"
     CDS             15607..15990
                     /gene="rplQ"
                     /locus_tag="ACHK49_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO9262295.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            16097..16465
                     /locus_tag="ACHK49_001627"
     CDS             16097..16465
                     /locus_tag="ACHK49_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO9262296.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16476..16901
                     /gene="zntR"
                     /locus_tag="ACHK49_001628"
     CDS             16476..16901
                     /gene="zntR"
                     /locus_tag="ACHK49_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO9262297.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16957..17175
                     /gene="arfA"
                     /locus_tag="ACHK49_001629"
     CDS             16957..17175
                     /gene="arfA"
                     /locus_tag="ACHK49_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO9262298.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(17172..17582)
                     /gene="mscL"
                     /locus_tag="ACHK49_001630"
     CDS             complement(17172..17582)
                     /gene="mscL"
                     /locus_tag="ACHK49_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO9262299.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(17712..19088)
                     /gene="trkA"
                     /locus_tag="ACHK49_001631"
     CDS             complement(17712..19088)
                     /gene="trkA"
                     /locus_tag="ACHK49_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO9262300.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(19110..20399)
                     /gene="rsmB"
                     /locus_tag="ACHK49_001632"
     CDS             complement(19110..20399)
                     /gene="rsmB"
                     /locus_tag="ACHK49_001632"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO9262301.1"
                     /translation="MKKQRNLRSMAAQAVEQIVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGVYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQEELLAGFYASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGTEEGDGFFYAKLIKK"
     gene            complement(20445..21392)
                     /gene="fmt"
                     /locus_tag="ACHK49_001633"
     CDS             complement(20445..21392)
                     /gene="fmt"
                     /locus_tag="ACHK49_001633"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO9262302.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21407..21916)
                     /gene="def"
                     /locus_tag="ACHK49_001634"
     CDS             complement(21407..21916)
                     /gene="def"
                     /locus_tag="ACHK49_001634"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO9262303.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            22046..23170
                     /gene="dprA"
                     /locus_tag="ACHK49_001635"
     CDS             22046..23170
                     /gene="dprA"
                     /locus_tag="ACHK49_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO9262304.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            23142..23616
                     /gene="smg"
                     /locus_tag="ACHK49_001636"
                     /pseudo
     CDS             23142..23616
                     /gene="smg"
                     /locus_tag="ACHK49_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
     gene            23645..24187
                     /locus_tag="ACHK49_001637"
     CDS             23645..24187
                     /locus_tag="ACHK49_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO9262305.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCLACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            24192..24764
                     /gene="tsaC"
                     /locus_tag="ACHK49_001638"
     CDS             24192..24764
                     /gene="tsaC"
                     /locus_tag="ACHK49_001638"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO9262306.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24769..25587
                     /gene="aroE"
                     /locus_tag="ACHK49_001639"
     CDS             24769..25587
                     /gene="aroE"
                     /locus_tag="ACHK49_001639"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO9262307.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25584..25841
                     /locus_tag="ACHK49_001640"
     CDS             25584..25841
                     /locus_tag="ACHK49_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO9262308.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25817..26371)
                     /locus_tag="ACHK49_001641"
     CDS             complement(25817..26371)
                     /locus_tag="ACHK49_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO9262309.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            26844..>26925
                     /locus_tag="ACHK49_001642"
     rRNA            26844..>26925
                     /locus_tag="ACHK49_001642"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6931 a         6385 c         6983 g         6626 t
ORIGIN      
        1 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
       61 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      121 ggtatcaaag agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      181 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      241 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      301 tctgaagtgt acattctgtc caaagatgaa ggcggccgtc atactccgtt cttcaaaggc
      361 taccgtccgc agttctactt ccgtactact gacgtgactg gtaccatcga actgccggaa
      421 ggcgtagaga tggtaatgcc gggcgacaac atcaaaatgg ttgttaccct gatccacccg
      481 atcgcgatgg acgacggtct gcgtttcgca atccgtgaag gcggccgtac cgttggcgcg
      541 ggcgttgttg ctaaagttct gggctaatta cacgttaatt agttttgaat tgaaaagggc
      601 gcttcggcgc cctttttgct gcccgtaatt tgagctcgaa atatttagtc gtaattttgc
      661 tcgctgaact atggcgcaaa taaaactgtg ctattgtaat cataaccatt ctcatttaca
      721 ctttgcgcag aatttgaacg ggagcaatca tgtacgtttg tttgtgtaat ggtgtaagtg
      781 acaaaaaaat tcgccaggca gtccgtcagt ttcatccgca atctttccaa caattgcgta
      841 agtttattcc tgtgggaaat caatgcggta agtgcattcg cgccgcgcgc gaagtgatgc
      901 aggacgagtt gatgcagatg ccagaattta aagagattgc ctgaagacca cgcttatttt
      961 ttgacatccc gctagcccca tctacgctct aatagtggaa gcggagggac tataaaatga
     1021 aaggtgatgt taaaatcata aattatctca ataaactatt gggaaatgag cttgtcgcaa
     1081 tcaatcagta ttttctccat gcccggatgt ttaaaaactg gggcctgatg cgtctcaatg
     1141 acgtcgaata ccatgaatcc atcgatgaga tgaaacacgc tgataaatat atcgagcgta
     1201 ttttgttttt agaaggaatc cccaacttac aggatttagg caaactgggg attggtgaag
     1261 atgtcgagga gatgctgcaa tccgacctgc gattagaact ggaaggcgcg aaggatctcc
     1321 gtgaggctat cgcgtatgcc gacagcgttc atgactatgt tagccgtgat atgatgatcg
     1381 agatccttgc cgatgaagaa ggtcatattg actggctcga aactgagctg gatctcatcg
     1441 gtaaaatcgg tttacaaaat tacctgcaat cacaaataaa agttaaagat taacggcctg
     1501 ctagcaaact atcccaacct acaacgaaac ccgcagccgc cagaaatggc ccaaacggca
     1561 gcgggttttt taatgactct tttcctctca tgagcagacc aacaactacg gctccgcaag
     1621 cgaatgaggc agcaaggaaa accagtcgtg gcaagaaggt ccaggtatgc caggcaccaa
     1681 gggcggcgag aaacttcaca tcaccatagc ctaatccttc tttatgacgc agtatgcgat
     1741 acccccagta aatgacagcg aatgtgccgt aaccaataat cgccccccat aacgcatcgg
     1801 ctaaaccgtc aggattacaa acctgagaga acaaaagacc tgaccagagt aacgggcagg
     1861 taaatctgtc aggtaacaag ccatgctttg catcccataa aaaaagtagg acagataaac
     1921 aagcgtacaa aatcagaaag ggaaggttag cgtccataaa acatcctctg taattggagt
     1981 atcaccatcg aataactccg gatgctcagc aacagagaaa tcgctgattt gttaacctgg
     2041 ttccggtgaa aaacgctatc ttcacaacct tacaaatttc gtggaataca gctcgcaatc
     2101 tgcgcgttgc gattaatttc tacccgatgc ttgcgtcagg ctcagatttg cgtataatgc
     2161 gcgggcttgt cgtagttgac agcaggttca atctgaacct cagttagccg aatttggcta
     2221 cctaacaatg ctcccaatcg gggagctacg taagaacggt tacactctcc catcaatcgt
     2281 aatgggtctg aggagtaatc attttcgttt ataaaataat tggagctctg gtctcatgca
     2341 gaaccaaaga atccgtatcc gcctgaaagc gtttgatcat cgtctgatcg atcaagcaac
     2401 cgcggaaatc gtcgagactg ccaagcgcac tggtgcgcag gtccgtggtc cgatcccgct
     2461 gccgacacgc aaagagcgct tcactgttct gatctccccg cacgtcaaca aagacgcgcg
     2521 cgatcagtac gaaatccgta ctcacttgcg tctggttgac atcgttgagc caaccgagaa
     2581 aaccgttgat gctctgatgc gtctggatct ggctgccggt gtagacgtgc agatcagcct
     2641 gggttaatca ggtcattgag cgattgagag gttgaaacaa tgattggttt agtcggtaaa
     2701 aaagtgggta tgacccgtat cttcacagaa gacggcgttt ctatcccagt aaccgtaatc
     2761 gaagttgaag caaaccgcgt tactcaggtt aaagacctgg ctaacgatgg ctaccgtgct
     2821 attcaggtga ccaccggtgc taaaaaagct aaccgtgtga ccaagcctga agctggccac
     2881 ttcgctaaag ctggcgtaga agctggccgt ggtctgtggg aattccgcct ggctgaaggc
     2941 gaagagttca ctgtaggtca gagcattagc gttgaactgt ttgctgacgt taaaaaagtt
     3001 gacgtaactg gcacctctaa aggtaaaggt ttcgcaggta ccgttaagcg ctggaacttc
     3061 cgtacccagg acgctactca cggtaactcc ttgtctcacc gcgttccggg ttctatcggt
     3121 cagaaccaga ctccgggcaa agtgttcaaa ggcaagaaaa tggcaggtca gatgggtaac
     3181 gaacgtgtaa ccgttcagag ccttgacgta gtacgcgttg acgctgagcg caacctgctg
     3241 ctggttaaag gtgctgtccc gggtgcaacc ggtagcgacc tgatcgttaa accagctgtg
     3301 aaggcgtaag gagatagcaa tggaattagt attgaaagac gcgcagagcg cgctgactgt
     3361 ttccgaaact accttcggtc gtgatttcaa cgaagcgctg gttcaccagg ttgttgttgc
     3421 ttatgcagct ggtgctcgtc agggtactcg tgctcagaag actcgtgctg aagtaactgg
     3481 ttctggtaaa aaaccgtggc gccagaaagg caccggccgt gcgcgttctg gttctatcaa
     3541 gagcccgatc tggcgttctg gtggcgtgac ctttgctgct cgtccgcagg accacagtca
     3601 aaaagttaac aagaagatgt accgcggcgc gctgaaaagc atcctgtccg aactggtacg
     3661 tcaggatcgt ctgatcgttg tcgagaagtt ctctgtagaa gcgccgaaaa ctaagctgct
     3721 ggcacagaaa ctgaaagaca tggctctgga agatgtgctg atcatcaccg gtgagctgga
     3781 cgaaaacctg ttcctggctg cgcgcaacct gcacaaggtt gacgtacgcg atgcaactgg
     3841 tatcgacccg gttagcctga tcgccttcga caaagtcgta atgactgctg atgctgttaa
     3901 gcaagttgag gagatgctgg catgattcgt gaagaacgtc tgctgaaggt gctgcgtgca
     3961 ccgcacgttt ctgaaaaagc gtctactgcg atggaaaaat ccaacaccat cgtactcaaa
     4021 gttgctaaag acgcgaccaa agcagaaatc aaagctgctg tgcagaaact gtttgaagtc
     4081 gaagtcgaag tcgttaacac cctggtagtt aaagggaaag ttaaacgtca cggacagcgt
     4141 atcggtcgtc gtagcgactg gaaaaaagct tacgtcaccc tgaaagaagg ccagaatctg
     4201 gacttcgttg gcggcgctga gtaagtcgga ggagtaatac aatggcagtt gttaaatgta
     4261 aaccgacatc tccgggtcgt cgccacgtag ttaaagtggt taaccctgag ctgcacaagg
     4321 gcaaaccttt tgctccgttg ctggaaaaaa acagcaaatc cggtggtcgt aacaacaatg
     4381 gccgtatcac cactcgtcat atcggtggtg gccacaagca ggcttaccgt attgttgact
     4441 tcaaacgcaa caaagacggt atcccggcag ttgttgaacg tcttgagtac gatccgaacc
     4501 gttccgcgaa catcgcgctg gttctgtaca aagacggtga acgccgttac atcctggccc
     4561 ctaaaggcct gaaagctggc gaccagattc agtctggcgt tgatgctgca atcaaaccag
     4621 gtaacaccct gccgatgcgc aacatcccgg ttggttctac tgttcataac gtagaaatga
     4681 aaccaggtaa aggcggtcag ctggcacgtt ccgctggtac ttacgttcag atcgttgctc
     4741 gtgatggtgc ttatgtcacc ctgcgtctgc gttctggtga aatgcgtaaa gtagaagcag
     4801 actgccgtgc aactctgggc gaagttggca atgctgagca tatgctgcgc gttctgggta
     4861 aagcaggtgc tgcacgctgg cgtggtgttc gtccgaccgt tcgcggtacc gcgatgaacc
     4921 cggtagacca cccacatggt ggtggtgaag gtcgtaactt tggtaagcac ccggtaactc
     4981 cgtggggcgt tcagaccaaa ggtaagaaga cccgcagcaa caagcgtact gataaattca
     5041 tcgtacgtcg ccgtagcaaa taattttaga ggataagcca tgccacgttc tctcaagaaa
     5101 ggtcctttta ttgacctgca cttgctgaag aaggtagaga aagcggtgga aagcggagac
     5161 aagaagcccc tgcgcacttg gtcccgtcgt tcaacgatct ttcctaacat gatcggtttg
     5221 accatcgctg tccataatgg tcgtcagcac gttccggtat ttgtaaccga cgaaatggtc
     5281 ggtcacaaac tgggtgaatt cgcaccgact cgtacttatc gcggccacgc tgctgataaa
     5341 aaagcgaaga agaaataagg taggaggaag agatggaaac tatcgctaaa catcgccatg
     5401 ctcgttcttc tgctcagaag gttcgccttg ttgctgacct gattcgcggt aagaaagtgt
     5461 cgcaggctct ggatattttg acctacacca acaagaaagc ggctgtactg gtcaagaaag
     5521 ttctggaatc tgccattgct aacgctgaac acaacgatgg cgctgacatt gacgatctga
     5581 aagttacgaa aattttcgta gacgaaggcc cgagcatgaa gcgcattatg ccgcgtgcaa
     5641 aaggtcgtgc agatcgcatc ctgaagcgca ccagccacat cactgtggtt gtgtccgatc
     5701 gctgagactc tggagactag caatgggtca gaaagtacat cctaatggta ttcgcctggg
     5761 tattgtaaaa ccatggaact ctacctggtt tgcgaacacc aaagaattcg ctgacaacct
     5821 ggacagcgat tttaaagtac gtcagtacct gactaaggaa ctggctaaag cgtccgtatc
     5881 tcgtatcgtt atcgagcgtc cggctaagag catccgtgta accattcaca ctgctcgccc
     5941 gggtatcgtt atcggtaaaa aaggtgaaga cgtagaaaaa ctgcgtaagg tcgtagcgga
     6001 catcgctggc gttcctgcac agatcaacat cgccgaagtt cgtaagcctg aactggacgc
     6061 aaaactggtt gctgacagca tcacttctca gctggaacgt cgcgttatgt tccgtcgtgc
     6121 tatgaagcgt gctgtacaga acgcaatgcg tctgggcgct aaaggtatta aagttgaagt
     6181 tagcggccgt ctgggcggcg cggaaatcgc acgtaccgaa tggtaccgcg aaggtcgcgt
     6241 accgctgcac actctgcgtg ctgacatcga ctacaacacc tctgaagcgc acaccactta
     6301 cggtgtaatc ggcgttaaag tgtggatctt caaaggcgag atcctgggtg gtatggctgc
     6361 tgttgaacaa ccggaaaaac cggctgctca gcctaaaaag cagcagcgta aaggccgtaa
     6421 ataaggagcg tcgctgatgt tacaaccaaa gcgtacaaaa ttccgtaaaa tgcacaaagg
     6481 ccgtaaccgc ggtctggcgc agggtacgga tgttagcttc ggcagcttcg gtctgaaagc
     6541 tgttggccgt ggtcgtctga ctgcccgtca gatcgaagca gcacgtcgtg ctatgacccg
     6601 tgcagttaag cgtcaaggta agatctggat ccgtgtgttc ccggacaaac cgatcactga
     6661 aaagccgctg gcagtgcgta tgggtaaagg taaaggtaac gtggagtatt gggttgcctt
     6721 gattcagccg ggtaaagtcc tgtatgaaat ggacggtgtt ccggaagagc tggcccgtga
     6781 agcattcaag ctggcagcag cgaaactgcc gattaaaacc acctttgtaa ctaagacggt
     6841 gatgtaatga aagcaaaaga gctgcgtgag aagagcgttg aagagctgaa caccgagctg
     6901 ctgaacctgc tgcgtgagca gttcaacctg cgtatgcagg ctgcaagtgg ccagctgcaa
     6961 cagtctcacc tgttgaagca agtgcgtcgc gatgtcgcac gcgttaagac tttactgaac
     7021 gagaaggcgg gtgcgtaatg accgataaaa tccgtactct gcaaggtcgc gttgttagcg
     7081 acaaaatgga gaaatccatt gttgttgcta tcgaacgttt tgtgaaacac ccgatctacg
     7141 gtaaattcat caagcgtacg accaaactgc acgtacatga cgagaacaac gaatgcggta
     7201 tcggtgacgt ggttgaaatc cgcgaatgcc gtccgctgtc caagactaaa tcctggacgc
     7261 tggttcgcgt tgtagagaaa gcggttctgt aatacagtac actctctcga tacgaataaa
     7321 cggctcagaa atgagccgtt tattttttct acccatatcc ttgaagcggt gttataatgc
     7381 cgcgccctcg atatggggat ttttaacgac ctgattttcg ggtctcagta gtagttgaca
     7441 ttagcggagc actaaaatga tccaagaaca gactatgctg aacgtcgccg acaactccgg
     7501 tgcacgtcgc gtaatgtgta tcaaggttct gggtggctcg caccgtcgct acgcaggcgt
     7561 aggcgacatc atcaagatca ccatcaaaga agcaattccg cgtggtaagg tcaaaaaagg
     7621 tgatgtgctg aaggcggtag tggtgcgcac caagaagggt gttcgtcgcc cggacggttc
     7681 tgtcattcgc ttcgatggta atgcttgtgt tcttctgaac aacaacagcg agcagcctat
     7741 cggtacgcgt atttttgggc cggtaactcg tgagcttcgt agtgagaagt tcatgaaaat
     7801 tatctctctg gcaccagaag tactctaagg agcgaatcat ggcagcgaaa atccgtcgtg
     7861 atgacgaagt tatcgtgtta accggtaaag ataaaggtaa acgcggtaaa gttaagaatg
     7921 tcctgtcttc cggcaaggtc attgttgaag gtatcaacct ggttaagaaa catcagaagc
     7981 cggttccggc cctgaaccaa ccgggtggca tcgttgaaaa agaagccgct attcaggttt
     8041 ccaacgtagc aatcttcaat gcggcaaccg gcaaggctga ccgtgtaggc tttagattcg
     8101 aagacggcaa aaaagtccgt ttcttcaagt ctaacagcga aactatcaag taatttggag
     8161 tagtacgatg gcgaaactgc atgattacta caaagacgaa gtagttaaaa aactcatgac
     8221 tgagtttaac tacaattctg tcatgcaagt ccctcgggtc gagaagatca ccctgaacat
     8281 gggtgttggt gaagcgatcg ctgacaaaaa actgctggat aacgcagcag cagacctggc
     8341 agcaatctcc ggtcaaaaac cgctgatcac caaagcacgc aaatctgttg caggcttcaa
     8401 aatccgtcag ggctatccga tcggctgtaa agtaactctg cgtggcgaac gcatgtggga
     8461 gttctttgag cgcctgatca ctattgctgt acctcgtatc cgtgacttcc gtggcctgtc
     8521 cgctaagtct ttcgacggtc gtggtaacta cagcatgggt gtccgtgagc agatcatctt
     8581 cccagaaatc gactacgata aagtcgaccg cgttcgtggt ttggatatta ccattaccac
     8641 tactgcgaaa tctgacgaag aaggccgcgc tctgctggct gcctttgact tcccgttccg
     8701 caagtaaggt agggttacta aatggctaag caatcaatga aagcacgcga agtaaaacgc
     8761 gtagctttag ctgataaata cttcgcgaaa cgcgctgaac tgaaagcgat catctctgat
     8821 gtgaacgctt ccgacgaaga tcgttggaac gctgttctca agctgcagac tctgccgcgt
     8881 gattccagcc cgtctcgtca gcgtaaccgc tgccgtcaaa caggtcgtcc gcatggtttc
     8941 ctgcggaagt tcgggttgag ccgtattaag gtccgtgaag ccgctatgcg cggtgaaatc
     9001 ccgggtctga aaaaggctag ctggtaattg tcaccaattg aatcacggga gtaaagacag
     9061 atgagcatgc aagatccgat cgcggatatg ctgacccgta tccgtaacgg tcaggccgcg
     9121 aacaaagctg cggtcaccat gccttcctcc aagctgaaag tggcaatcgc caacgtgctg
     9181 aaggaagaag gttttattga agattttaaa gttgaaggcg acaccaagcc tgaactggaa
     9241 cttactctga agtatttcca gggcaaagct gttgtagaaa gcattcagcg tgtcagccgc
     9301 ccaggtctgc gcatctataa acgtaaagat gagctgccga aagttatggc gggtctgggt
     9361 atcgcagttg tttctacctc taaaggtgtt atgactgatc gtgcagcgcg ccaggctggt
     9421 cttggtggcg aaattatctg ctacgtagcc taatcggagg aaaaaatgtc tcgtgttgct
     9481 aaagcaccgg tcgttgttcc tgccggcgtt gacgtaaaaa tcaacggtca ggttattacg
     9541 atcaaaggta aaaacggcga gctgactcgt actctcaacg atgctgttga agttaaacat
     9601 gcagataata ccctgacctt cggtccgcgt gatggttacg cagacggttg ggcacaggct
     9661 ggtaccgcgc gtgccctgct gaactcaatg gttatcggtg ttaccgaagg cttcactaag
     9721 aagctgcagc tggttggtgt aggttaccgt gcagcggtta aaggcaatgt gattaacctg
     9781 tctctgggtt tctctcatcc tgttgaccat cagctgcctg cgggtatcac tgctgaatgt
     9841 ccgactcaga ctgaaatcgt gctgaaaggc gctgataagc aggtgatcgg ccaggttgca
     9901 gcggatctgc gcgcctaccg tcgtcctgag ccttataaag gcaagggtgt tcgttacgcc
     9961 gacgaagtcg tgcgtaccaa agaggctaag aagaagtaag gtaacactat ggataagaaa
    10021 tctgctcgta tccgtcgtgc gacccgcgca cgccgcaagc tccaggagct gggcgcaact
    10081 cgcctggtgg tacatcgtac cccgcgtcac atttacgcac aggtaattgc accgaacggt
    10141 tctgaagttc tggtagctgc ttctactgta gaaaaagcta tcgctgaaca actgaagtac
    10201 accggtaaca aagacgcggc tgcagctgtg ggtaaagctg tcgctgaacg cgctctggaa
    10261 aaaggcatca aagatgtatc ctttgaccgt tccgggttcc aatatcatgg tcgtgtccag
    10321 gcactggcag atgctgcccg tgaagctggc cttcagttct aaggtagagg tgtaagatgg
    10381 ctcacatcga aaaacaagct ggcgaactgc aggaaaagct gatcgcggta aaccgcgtat
    10441 ctaaaaccgt taaaggtggt cgtattttct ccttcacagc tctgactgta gttggcgatg
    10501 gtaacggtcg cgttggtttt ggttacggta aagcgcgtga agttccagca gcgatccaga
    10561 aagcgatgga aaaagcccgt cgcaatatga ttaacgtcgc gctgaataac ggcactctgc
    10621 aacaccctgt taaaggtgtt cacacgggtt ctcgcgtatt catgcagccg gcttccgaag
    10681 gtaccggtat catcgccggt ggtgcaatgc gcgccgttct ggaagtcgct ggggttcata
    10741 acgttctggc taaagcatat ggttccacca acccgatcaa cgtggttcgt gcaactattg
    10801 atggcctgga aaatatgaat tctccagaaa tggtcgctgc caagcgtggt aaatccgttg
    10861 aagaaattct ggggaaataa accatggcaa agactattaa aattactcaa acccgcagtg
    10921 caatcggtcg tctgccgaaa cacaaggcaa cgctgcttgg cctgggtctg cgtcgtattg
    10981 gtcacaccgt agagcgcgag gatactcctg ctattcgcgg tatgatcaac gcggtttcct
    11041 tcatggttaa agttgaggag taagagatgc gtttaaatac tctgtctccg gccgaaggct
    11101 ccaaaaaggc gggtaaacgc ctgggtcgtg gtatcggttc tggcctcggt aaaaccggtg
    11161 gtcgtggtca caaaggtcag aaatctcgtt ctggcggtgg cgtacgtcgc ggtttcgagg
    11221 gtggtcagat gcctctgtac cgtcgtctgc cgaaattcgg cttcacttct cgtaaagcag
    11281 cgattacagc cgaagttcgt ctgtctgacc tggctaaagt agaaggcggt gttgtagacc
    11341 tgaacacgct gaaagcggct aacattatcg gtatccagat cgagttcgcg aaagtgatcc
    11401 tggctggcga agtaacgact ccggtaactg ttcgtggcct gcgtgttact aaaggcgctc
    11461 gtgctgctat cgaagctgct ggcggtaaaa tcgaggaata agtagcagat ggctaaacaa
    11521 ccgggattag attttcaaag tgccaaaggt ggcttaggcg agctgaaacg cagactgctg
    11581 tttgttatcg gtgcgctgat tgtgttccgt attggctctt ttattccgat ccctggtatt
    11641 gatgccgctg tacttgccaa actgcttgag caacagcgag gcaccatcat tgagatgttt
    11701 aacatgttct ctggtggtgc tctcagccgt gcttctatct ttgctctggg gatcatgccg
    11761 tatatttcgg cgtcgatcat tatccagctg ctgacggtag ttcacccaac gttggcagaa
    11821 attaagaaag aaggggagtc tggtcgtcgt aagatcagcc agtacacccg ttacggtact
    11881 ctggtgctgg caatattcca gtcgatcggt attgctaccg gtctgccgaa tatgcctggt
    11941 atgcaaggcc tggtgattaa cccaggcttt gcattctact tcaccgctgt tgtaagtctg
    12001 gtcacaggaa cgatgttcct gatgtggttg ggcgaacaga ttactgaacg aggtatcggc
    12061 aacggtattt caatcattat cttcgccggt attgtcgcgg gactcccgcc agccattgcc
    12121 catactatcg agcaagcgcg tcaaggcgac ctgcacttcc tcgtgttgct gttggttgca
    12181 gtattagtat ttgcagtgac gttctttgtt gtatttgttg agcgtggtca acgccgcatt
    12241 gtggtaaact acgcgaaacg tcagcaaggt cgtcgtgtct atgctgcaca gagcacacat
    12301 ttaccgctga aagtgaatat ggcgggggta atcccggcaa tcttcgcttc cagtattatt
    12361 ctgttcccgg cgaccatcgc gtcatggttc gggggcggta ctggttggaa ctggctgaca
    12421 acaatttcgc tgtatttgca gcctgggcaa ccgctttatg tgttactcta tgcgtctgca
    12481 atcatcttct tctgtttctt ctacacggcg ttggttttca acccgcgtga aacagcagat
    12541 aacctgaaga agtccggtgc atttgtacca ggaattcgtc cgggagagca aacggcgaag
    12601 tatatcgata aagtaatgac ccgcctgacc ctggttggtg cgctgtatat tacctttatc
    12661 tgcctgatcc cggagttcat gcgtgatgca atgaaagtac cgttctactt cggtgggacc
    12721 tcactgctta tcgttgttgt cgtgattatg gactttatgg ctcaagtgca aactctgatg
    12781 atgtccagtc agtatgagtc tgcattgaag aaggcgaacc tgaaaggcta cggccgataa
    12841 ttggtcgccc gagaagttac ggagagtaaa aatgaaagtt cgtgcttccg tcaagaaatt
    12901 atgccgtaac tgcaaaatcg ttaagcgtga tggtgtcatc cgtgtgattt gcagtgccga
    12961 gccgaagcat aaacagcgcc aaggctgatt ttttcgcata tttttcttgc aaagttgggt
    13021 tgagctggct agattagcca gccaatcttt tgtatgtctg tgcgtttcca tttgagtatc
    13081 ctgaaaacgg gcttttcagc atggaacgta catattaaat agtaggagtg catagtggcc
    13141 cgtatagcag gcattaacat tcctgatcat aagcatgccg taatcgcatt aacttcgatt
    13201 tatggcgtcg gcaagacccg ttctaaagcc atcctggctg cagcgggtat cgctgaagat
    13261 gttaagatca gtgagctgtc tgaaggacaa atcgacacgc tgcgtgacga agttgccaaa
    13321 tttgtcgttg aaggtgatct gcgccgtgaa atcagcatga gcatcaagcg cctgatggat
    13381 cttggttgct atcgcggttt gcgtcatcgt cgtggtctcc cggttcgcgg tcagcgtacc
    13441 aagaccaacg cacgtacccg taagggtccg cgcaaaccga tcaagaaata atcggggtga
    13501 ttgaataatg gcaaaggcac caattcgtgc acgtaaacgt gtaagaaaac aagtctctga
    13561 cggcgtggct catatccatg cttctttcaa caacaccatc gtgactatca ctgatcgtca
    13621 gggtaacgcg ttgggttggg caacagccgg tggttccggt ttccgtggtt ctcgcaaatc
    13681 cactccgttt gcagctcagg ttgcagcaga gcgttgcgct gacgccgtga aagaatacgg
    13741 catcaagaat ctggaagtta tggttaaagg tccgggtcca ggccgcgaat ctactattcg
    13801 tgctctgaac gccgcaggtt tccgcatcac taacattact gatgtgactc cgatccctca
    13861 taacggttgt cgtccgccga aaaaacgtcg cgtataacgc ctcgttttcc aggtttgttg
    13921 gagaaagaaa atggcaagat atttgggtcc taagctcaag ctgagccgtc gtgagggcac
    13981 cgacttattc cttaagtctg gcgttcgcgc gatcgatacc aagtgtaaaa ttgaacaagc
    14041 tcctggccag cacggtgcgc gtaaaccgcg tctgtctgac tatggtgtgc agttgcgtga
    14101 aaagcaaaaa gttcgccgta tctatggtgt gctggagcgt cagttccgta actactacaa
    14161 agaagcagca cgtctgaaag gcaacaccgg tgaaaacctg ttggctctgc tggaaggtcg
    14221 tctggacaac gttgtatacc gtatgggctt cggtgccact cgtgcagaag cacgtcagct
    14281 ggttagccat aaagcaatta tggtaaacgg tcgtgttgtt aacatcgctt cttatcaggt
    14341 tagtccgaat gacgttgtaa gcattcgtga gaaagcgaag aagcagtctc gcgtgaaagc
    14401 cgctctggag ctggctgagc agcgtgaaaa gccaacctgg ctggaagttg atgctggcaa
    14461 gatggaaggt acgtttaagc gtaagccgga gcgttctgat ctgtctgcgg acattaacga
    14521 acacctgatc gtcgagcttt actccaagta aagcttagta ccaaagagag gacacaatgc
    14581 agggttctgt gacagagttt ctaaaaccgc gcctggttga tatcgagcaa gtgagttcga
    14641 cgcacgccaa ggtgaccctt gagcctttag agcgtggctt tggccatact ctgggtaacg
    14701 cactgcgccg tattctgctc tcatcgatgc cgggttgcgc ggtgaccgag gttgagattg
    14761 atggtgtact acatgagtac agcaccaaag aaggcgttca ggaagatatc ctggaaatcc
    14821 tgctcaacct gaaagggctg gcggtgagag ttcagggcaa agatgaagtt attcttacct
    14881 tgaataaatc tggcattggc cctgtgactg cagccgatat cacccacgac ggtgatgtcg
    14941 aaatcgtcaa gccgcagcac gtgatctgcc acctgaccga tgagaacgcg tctattagca
    15001 tgcgtatcaa agttcagcgc ggtcgtggtt atgtgccggc ttctacccga attcattcgg
    15061 aagaagatga gcgcccaatc ggccgtctgc tggtcgacgc atgctacagc cctgtggagc
    15121 gtattgccta caatgttgaa gcagcgcgtg tagaacagcg taccgacctg gacaagctgg
    15181 tcatcgaaat ggaaaccaac ggcacaatcg atcctgaaga ggcgattcgt cgtgcggcaa
    15241 ccattctggc tgaacaactg gaagctttcg ttgacttacg tgatgtacgt cagcctgaag
    15301 tgaaagaaga gaaaccagag ttcgatccga tcctgctgcg ccctgttgac gatctggaat
    15361 tgactgtccg ctctgctaac tgccttaaag cagaagctat ccactatatc ggtgatctgg
    15421 tacaacgtac cgaggttgag ctccttaaaa cgcctaacct tggtaaaaaa tctcttactg
    15481 agattaaaga cgtgctggct tcccgtggac tgtctctggg catgcgcctg gaaaactggc
    15541 caccggcaag catcgctgac gagtaaccgg atcacaggtt aaggttttac tgagaaggat
    15601 aaggtcatgc gccatcgtaa gagtggtcgt caactgaacc gcaacagcag ccatcgccag
    15661 gctatgttcc gcaatatggc aggttcactg gttcgtcatg aaatcatcaa gacgactctg
    15721 cctaaagcga aagagctgcg ccgcgtagtt gagccgctga ttactcttgc caagactgat
    15781 agcgttgcta atcgtcgtct ggcattcgcc cgtactcgtg ataacgagat cgtggcaaaa
    15841 ctgtttaacg aactgggccc gcgtttcgcg agccgtgccg gtggttacac tcgtattctg
    15901 aagtgtggct tccgtgcagg cgacaacgcg ccgatggctt acatcgagct ggttgatcgt
    15961 tcagagaaag cagaagctgc tgcagagtaa tctgaagcaa cgtaaaaaaa cccgccccgg
    16021 cgggtttttt tatacccgta gcatccccac ttatctacaa tagctgtact ctttttgttc
    16081 atcccctgga gtatttatgt ggttacttga ccagtgggca gagcgtcata tagcagaagc
    16141 gcaagcgaaa ggtgagtttg ataacctggc aggtagcggc gaaccattga tactggatga
    16201 tgattctcat gtgccaccgg aattacgtgc ggggtatcgc ttgctgaaga atgccggttg
    16261 cttaccgcca gaacttgagc agcggaggga agcaattcag cttctggata tcctgagagg
    16321 tatccgtcac gatgatccgc aatatcaaga ggttagccgt cgattgtcat tactggaatt
    16381 gaagctgcga caagctggat tgagtaccga ttttttacgc ggcgattatg ctgacaagtt
    16441 gttggacaaa atcaacgata actagtggag tatgtatgta tcgcattggt gagctggcaa
    16501 aaatggcgga agtaacacct gacacgattc gttattacga aaaacagcag atgatggagc
    16561 atgaagtgcg tactgaaggc gggtttcgcc tgtataccga aagcgatctc cagcgattga
    16621 aatttatccg ccatgccaga caactaggtt tcagtctgga gtcgatccgc gagttgctgt
    16681 cgatccgcat cgatcctgaa caccatacct gtcaggagtc aaaaggcatt gtgcaggaaa
    16741 gattgcagga agtcgaagca cggatagccg agttgcagag tatgcagcgt tccttgcaac
    16801 gccttaacga tgcctgttgt gggaccgccc atagcagtgt ttattgttcg attcttgaag
    16861 ctcttgaaca aggggcgagt ggcgttaaga gtggctgttg attttttgca ctgacaggat
    16921 tacactcgcg ccgtaaaata accaactgga gtttttatga gccgatatca gcatactaaa
    16981 gggcagataa aggataatgc gatagaagca ttactacatg atcccttatt ccgacagcga
    17041 gtagagaaaa ataagaaagg gaaaggcagt tacatgcgaa aaggaaaaca tggcaatcgg
    17101 gggaactggg aggccagtgg caagaaagta aatcactttt ttaccactgg tcttctgctt
    17161 tcaggcgctt gttaagagcg gttattctgc tctttcagca aatcacgaat ttctgccagt
    17221 aatacttctt ctttagttgg tgcaggtgcg gcagctggtt cttctttttt ccgattcagt
    17281 ttgttgatta gcttaatcgc cataaagatg gcaaaggcca caatcagaaa atcaaagacg
    17341 ttttgaatga agacaccgta atgcatcaca acagcaggga tatccccctg cgcatcgcgt
    17401 agcgtgacag caaactgttt aaaatcgatc ccgccaatta ataagcccag aggaggcatg
    17461 atgatatcgg caaccagtga agagacaatc ttcccgaatg ccgcaccgat aatgacaccc
    17521 accgccaaat ccaccacgtt cccgcgcatc gcaaattcgc gaaattcttt aataatgctc
    17581 atgttattct ccctatgaag ccgacaacca taagtctaac aaatgttaag ccattttcct
    17641 gccaggaatg aatttaatta atccttgata atcaaaggga aataataagg cgtcattaga
    17701 cgccttatta attacaagaa gaaagggctt ggttggaaga gtctttcgac gtcggtaata
    17761 aactttttat ctgtgaggaa cataattacg tgatcgcctt gctcaatgcg cagattgtca
    17821 ttggcaatca tcacgtcgtt tccacgtacc accgctccaa taatcgttcc tggcggtagc
    17881 ttgatttcgt caatgactct gccgacaacg cgtgaggtgc tttcatcacc gtgagcaacg
    17941 gcttcaatag cttctgctac gccgcggcgc aatgaggaaa caccaacaat atctgctttt
    18001 cgcacatggc taagcaacgc agaaatagtt gcttgttgtg gtgaaatcgc aatatcgatc
    18061 acactcccct gaaccagatc cacataagcg cgacgctgga tcaataccat cacctttttc
    18121 gcacccatac gtttggcaag catggcggac atgatattgg cctcgtcatc gttggtgacg
    18181 gcaataaaca gatcaacttg atcgatatgt tcttcggcca gcagttcttg atccgacgca
    18241 tcaccaaaaa agacgatcgt attctgcaac ttttccgcca gttcggcagc gcgctgctga
    18301 ttacgttcga tgagtttaac gctgtaatct ttttccagac gacgcgccag ccctgcaccg
    18361 atattaccgc caccaaccag catgatccgc ttatacggtt tttccagtcg ctgtaattca
    18421 ctcatcaccg cacggatatg ctgtgaagcg gcaataaaga acacttcatc accagcttcc
    18481 acaatggtcg aaccttgtgg acgaatgggg cgatcgtggc ggaaaattgc tgccacacga
    18541 gtatcgatat gtggcatatg ttcgcgcatg gtcgaaagtg cgttaccaat cagcgggccg
    18601 ccataatagg ctttaaccac agcgaggctg actttaccct cagcgaagtt caccacctgc
    18661 aatgcgccgg gatactcaat cagtcggtaa atattatcga taaccaactg ctctggtgcg
    18721 atcagatgat caatcggtac agcatctgaa tgaaatagct tatcggcatc gcgcacgtag
    18781 tctggtgagc ggatgcgagc gatgcgatta ggggtgttga aaagtgagta ggctacctgg
    18841 caggcaacca tattggtttc atctgaactg gttacagcaa ccagcatatc ggcgtcgtcg
    18901 gcacctgcct cccgcaatac gcgtggatga gagccatgcc cctgcacgac tcgcaggtca
    18961 aatttatcct gcaaggtccg cagacgctca ccgttggtat cgacaacagt gatatcgttg
    19021 ttctcgccaa ccaggttttc cgccagtgtg ccgccaacct ggccggcacc cagaatgata
    19081 attttcatca gtcgcgaccc gttatctcat cactttttga ttagcttagc gtaaaagaag
    19141 ccgtcgccct cttcggtacc aggcagattt tgtttacccg gttgctctgg tgttcctgtt
    19201 tcgcaaagtt cggcatcagc ggtacgttgc aaaaaggctt taatctgcag gctattctct
    19261 tccggtaaca ccgaacaggt ggcatagacc agggttccac cggattttaa atgcggccaa
    19321 atggcgtcga gaatttcaga ctgcaattgc gcgagttccg ggatatcgcg atcgcgacgt
    19381 aaccatttaa tatccggatg gcgacgaatc acaccggttg ctgaacaagg cgcatctaat
    19441 aaaatgcgat caaactgttg ctcgccacac cattgggaag ggtagcggcc atcaccttgt
    19501 ttcacggtcg ctttcatacc aaggcgtttt aaattgtcgt aaacgcgaga gaggcgctgt
    19561 tcgtcaatat caaccgcaac aacctgcgct tctggtgcca cctcaaggat atgcgttgtt
    19621 ttaccgccgg gggcggcaca aagatccaga atgtgttcac cgttttgtgg cgcaagccag
    19681 gtcatgcaac cttgtgctga tgcatcctga acggtaaccc atccgtcttc aaaaccaggt
    19741 agcgcatgaa caggtgcagg tgtttccagc cgtacagcat cagggtaatc cgcatgcggg
    19801 aaacctttca ttcctgcttc atccaacaat gcaagccagc tgtcgcggga atgatgcgta
    19861 cgattaacgc gcagccacat tggcggacgc tggttattag cttcgacgat ggattgccac
    19921 tgctctggat acgctttttg cagacgcttc agcaaccagg aaggatgcag ataacgtgca
    19981 tcactggcat aaaacccggc taataactct tcttgctgac gctggaactg gcgtaataca
    20041 ccgttaatca gccccttaag ttgcgggcgc ttaattgcga cagcgccttc aaccgtttca
    20101 gccagcgcag catgaggtgg aatgcgggta taaagcagtt gatacacacc aaccataatc
    20161 aggtaatgca cggtccgctg tttgccggtc atcggacggg ccattaactt attaatcagc
    20221 cagtctaact gtgaaagcgt acgcagtacg ccaaagcaca actcttgaag aagtgctttg
    20281 tctttatcgg aaactttttg ctggagcggt ggcagaatgt tgcttaatga ttgcccttgc
    20341 tcgacgattt gttcaacggc ctgtgccgcc atgctacgta aattacgttg ttttttcata
    20401 agtataaaaa tgcccggtaa gaccgggctt agaagagtgg actatcagac cagacggttg
    20461 cccggaacaa accattcccg acgagaattc aggaggtctt gcgcgctcat cgctttctta
    20521 cccgcaggtt gtaacgagag caggttcagg atgccatcac cagtcgcaac ctgaatgcct
    20581 tgtttattgg cttcaaggat cgttcctggt gcagcgttgg ttgccgtatc aatgaccgac
    20641 gctttccaga ctttaaccgg ttgtccttca atttccagcc agctcattgg ccatgggttg
    20701 aaagcgcgaa tgcagcgttc aagctgcgct gccgaaagcg accagtcaat acgcgcttct
    20761 tctttactca acttctcggc gtaagtgaca agagtttcgt cctgaacttc tggtttcgcc
    20821 gtgccgtctg ctagttgttt caacgtggtg ataagccctt gtgggccaag ctctgccagc
    20881 ttgtcgtaca gcgtaccact ggtatcttct gcagtaatcg ggcaggagag cttataaagc
    20941 atgtcaccgg tgtctaaacc aacatccatt tgcataatgg tcacaccagt ttctgcatca
    21001 cccgcccata gtgagcgttg gattggtgca gcaccgcgcc agcgtggcag cagtgaacca
    21061 tgaacgttga tacagccaag acgcggcatc tccagcactg ctttaggcag aattaagcca
    21121 taggcgacga cgaccataac atcagcctgc agatcggcga ccagttgctg gctttcttgt
    21181 ggacgcaggg aaacaggttg aaaaacgggc agaccttttt cctcagccag aactttaacc
    21241 gggctgggca tcagtttttt accgcgtcct gccggtcggt ctggctgggt gaacacgcca
    21301 acgacgttat gaccagaaga caacagcgcg tcgagatgac gcgctgcaaa gtcaggtgta
    21361 cccgcaaaaa taatacgtag tgattctgac acgttagttc ttatccttaa gcccgggctt
    21421 tcagacgatc tagtttttca actttctgac gaatacgttg ttgtttcagc ggtgacagat
    21481 aatccataaa cagtttgccg accaggtgat ccatctcatg ctgaatacag atggctaaca
    21541 gcccctctgc ttccagttca aatggtttac cgtcgcggtc aagggcgcga attttaactt
    21601 tctctgcgcg cggcactaaa gcacgttgtt cagggatcga caggcaacct tcttcaatgc
    21661 ctgtttcgcc gcttttttct aaaagctccg gattgattaa caccagccgt tcgtcacggt
    21721 tttccgaaac atcaataaca atgatacgtt gatggatatc aacctgggtt gccgccaggc
    21781 caataccttc ttctgcgtac atcgtctcga acatatcatc gacgatacgc tgtatttctg
    21841 cattcacttc ttctaccggt ttagcaactt tgcgaagccg ctcgtccgga atatgtaaca
    21901 cttgcaaaac tgacataaat ctccagagat gtgttcagga gttagaaaga ttatttcttc
    21961 tattctagac aaatccccct ctgattgaca gcatcactga ccaatcgcaa agattgctaa
    22021 ggctgcttat ggcagggaga taaggatggt cgatacagaa atttggctgc gtttaatgag
    22081 tatcagcagc ttgtacggcg atgatatggt ccgtatagct cactggctgg caaaacagtc
    22141 gcaaattgat gcggttggat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
    22201 tctttcattt ccgcgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
    22261 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttctggcga cgacagatta
    22321 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
    22381 agtggggagt cgggcgcatt catggtatgg cgagcgatgg ggacggttat tttgcgaaac
    22441 tctggcgacg cgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggc
    22501 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
    22561 taccattcat ccccgccgcc atgcccgact ggctgccagt ctgcttgaac atggcggagc
    22621 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
    22681 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
    22741 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
    22801 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
    22861 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
    22921 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
    22981 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
    23041 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
    23101 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
    23161 atttgtttga aacctatatt cacacagaag ctgagttgcg tgtggatcaa gacaaacttg
    23221 aacaggatct taccgacgca ggatttgatc gagaagatat ctacaatgcc ctgctttggc
    23281 tggaaaagct tgcggattat caggaaggac tggcagaacc gatgcaactg gcctctgatc
    23341 cactctccaa tgcgtattta tacccctgag gagtgtgaaa gactggatgc cagctgtcgt
    23401 ggttttctgc ttttccttga gcaaattcag gtgcttaacc ttgaaacccg tgaaatggtg
    23461 atagagcgag tgctcgcgct ggataccgca gagttcgacc tggaagacct gaaatgggta
    23521 atcctgatgg tgttgttcaa tattccgggc tgcgaaaatg cgtaccagca aatggaagaa
    23581 ttactctttg aagtgaatga aggtatgctg cattaatcat ttctttaatt cagcataagt
    23641 tgttatggcg aaatcagcac tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg
    23701 ggctgaactg gttattcgat ccgggaaaca cggtccgttt cttggatgct cacagtatcc
    23761 ggcgtgtgac tacgtccgtc ctctgaaatc ttcagcggat ggacatattg tcaaagttct
    23821 ggaggggcag gtttgccttg catgtggcgc aaatctggta ttacgccagg gacgctttgg
    23881 tatgtttatt ggttgcagta actaccctga atgcgaacat accgaactta tcgataaacc
    23941 ggacgaaaca gcaattacat gcccccaatg ccggacgggc catctggtcc agcgccgctc
    24001 ccgttatggc aaaacatttc actcctgtga tcgctacccg gagtgtcaat ttgccattaa
    24061 cttcaaaccc atagctggag aatgccctga gtgtcattat ccactactca tcgaaaagaa
    24121 aaccgcgcag ggtgtaaaac acttttgtgc cagtaaacaa tgtggaaagc cggtttcggc
    24181 ggaataataa cgtgaataat aacctgcaag gagacgctat cgcagctgcg atagatgtcc
    24241 tcaatgaaga acgtgtcatc gcctatccaa cggaagccgt tttcggtgtt gggtgcgatc
    24301 ctgatagcga aacagcagtg atgagactgt tggagttaaa acagcgtccg gttgataagg
    24361 ggctgatttt aatcgcggca aattacgagc agcttaaacc ctatattgat gacaccatgc
    24421 tgactgatgc gcagcgtgaa accatttttt cccgctggcc aggtcctgtc acctttgtct
    24481 ttcccgcgcc tgcgacaaca ccgcgctggt tgacgggccg ctttgattcg cttgctgtac
    24541 gagtcaccga ccatccgttg gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt
    24601 ctaccagtgc caacttgagt ggattgccac cttgtcgaac agtagacgaa gttcgcgcac
    24661 aatttggcgc ggcgttcccg gttgtgcctg gtgaaacggg ggggcgttta aatccttcag
    24721 aaatccgcga tgccctgacg ggtgaactgt ttcgacaggg gtaacataat ggaaacctat
    24781 gctgtttttg gtaatccgat agcccacagc aaatcgccat tcattcatca gcaatttgct
    24841 cagcaactga atattgaaca tccctatggg cgcgtgttgg cacccatcaa tgatttcatc
    24901 aacacactga acgctttctt tcgtgctggt ggtaaaggtg cgaatgtgac ggtgcctttt
    24961 aaagaagagg cttttgccag agcggatgag cttactgaac gggcagcgtt ggctggtgct
    25021 gttaataccc tcaagcggtt agaagatgga cgcctgctgg gtgacaatac cgatggtata
    25081 ggtttgttaa gcgatctgga acgtctgtct tttatccgtc ctggtttacg tattctgctc
    25141 atcggcgctg gtggagcatc tcgcggcgta ctactgccac ttctttccct ggactgtgcg
    25201 gtgacaatca ctaatcggac ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac
    25261 actggcagta ttcaggcgtt gggtatggac gaactggaag gtcatgagtt tgatctcatt
    25321 attaatgcaa catccagtgg catcagtggt gatattccgg cgatcccgtc atcgctcatt
    25381 catccaggca tttattgcta tgacatgttc tatcagaaag gaaaaactcc ttttctggca
    25441 tggtgtgagc agcgaggctc aaagcgtaat gctgatggtt taggaatgct ggtggcacag
    25501 gcggctcatg cctttcttct ctggcacggt gttctgcctg acgtagaacc agttataaag
    25561 caattgcagg aggaattgtc cgcgtgaatc aggccatcca gtttccggac agagaagagt
    25621 gggacgagaa taaaaaatgt gtatgttttc ccgctctcgt gaatggcatg caactgacat
    25681 gtgcgatctc tggcgagagt ctggcgtatc gttttactgg agatacgcca gaacagtggt
    25741 tagcgagttt tcgtcagcat cgctgggatc tggaagaaga agcggaaaac ttaattcagg
    25801 aacaaagtga agatgatcaa ggctgggtct ggttaccctg atccagatat tcgtccttcc
    25861 atttcacgta attattcgcg gaatagcgta acccagcctt ctcttcatca cttaacgggc
    25921 ggatctgttt gacggggcta ccgagataca gatatccgct ctccagccgt ttattttgtg
    25981 ggaccagact acccgcacca atcatcacat catcttctac tattgcgcca tcaagtaaaa
    26041 ttgagcccat cccaaccaaa actcgattgc caatggtgca gccgtggagc atcaccttgt
    26101 gaccaacagt gacatcttcg ccaatggtta atgggttgcc atctgggttg tacgaggatt
    26161 tatgagtgac atgcaacata ctgccatcct ggatattggt gcgtgctccg atctgtacat
    26221 aatgtacatc tccacgaatc acaacgagcg gccagatccc cacatcatca gccagacgaa
    26281 cgtcaccaat cacgacactg ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa
    26341 gatcgcggta tgggcgtaaa acatcagaca tacttacctc agcaataaat gatttactaa
    26401 tgactttggg ggcattattg gccttgtgca agtcttttag tatgcaaaaa agcaccgttt
    26461 tgtgtgcgaa tgcagcaaaa agggtgaaaa aacaacaaac agaaaaaaag atcaaaaaag
    26521 tacttgtgca aaaaattggg atccctataa tgcgcctccg ttgagacgac aacgtgaaac
    26581 acttcacaag atggtcgtaa caacgaagag aaaaaatcct gaaattaagg gttgactctg
    26641 aaagaggaaa gcgtaatata cgccacctcg cgacagtgag ctgaaagccg cgtcgcaact
    26701 gctctttaac aatttatcag acaatctgtg tgggcactcg aagatacgga ttcttaacgt
    26761 cgcaagacga aaaatgaata ccaagtctca agagtgaaca cgtaattcat tacgaagttt
    26821 aattctttga gcatcaaact tttaaattga agagtttgat catggctcag attgaacgct
    26881 ggcggcaggc ctaacacatg caagtcgaac ggtaacagga agaag
//
LOCUS       DAZGLJ010000043        26218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000043 DAZGLJ010000000
VERSION     DAZGLJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.043"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(18..206)
                     /locus_tag="ACHK49_001643"
     CDS             complement(18..206)
                     /locus_tag="ACHK49_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO9262310.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(210..569)
                     /gene="yeaR"
                     /locus_tag="ACHK49_001644"
     CDS             complement(210..569)
                     /gene="yeaR"
                     /locus_tag="ACHK49_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO9262311.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(742..1380)
                     /gene="leuE"
                     /locus_tag="ACHK49_001645"
     CDS             complement(742..1380)
                     /gene="leuE"
                     /locus_tag="ACHK49_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO9262312.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(1507..2430)
                     /gene="dmlR"
                     /locus_tag="ACHK49_001646"
     CDS             complement(1507..2430)
                     /gene="dmlR"
                     /locus_tag="ACHK49_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO9262313.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            2533..3618
                     /gene="dmlA"
                     /locus_tag="ACHK49_001647"
     CDS             2533..3618
                     /gene="dmlA"
                     /locus_tag="ACHK49_001647"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO9262314.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            3869..5479
                     /gene="yeaV"
                     /locus_tag="ACHK49_001648"
     CDS             3869..5479
                     /gene="yeaV"
                     /locus_tag="ACHK49_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO9262315.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            5511..6635
                     /gene="yeaW"
                     /locus_tag="ACHK49_001649"
     CDS             5511..6635
                     /gene="yeaW"
                     /locus_tag="ACHK49_001649"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO9262316.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSIELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            6691..7656
                     /gene="yeaX"
                     /locus_tag="ACHK49_001650"
     CDS             6691..7656
                     /gene="yeaX"
                     /locus_tag="ACHK49_001650"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO9262317.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(7710..8825)
                     /gene="rnd"
                     /locus_tag="ACHK49_001651"
     CDS             complement(7710..8825)
                     /gene="rnd"
                     /locus_tag="ACHK49_001651"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO9262318.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKVFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPALISGWRGELMAEALHNLLQEYPQ"
     gene            complement(8907..10592)
                     /gene="fadD"
                     /locus_tag="ACHK49_001652"
     CDS             complement(8907..10592)
                     /gene="fadD"
                     /locus_tag="ACHK49_001652"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO9262319.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(10797..11378)
                     /gene="yeaY"
                     /locus_tag="ACHK49_001653"
     CDS             complement(10797..11378)
                     /gene="yeaY"
                     /locus_tag="ACHK49_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO9262320.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(11418..12113)
                     /gene="tsaB"
                     /locus_tag="ACHK49_001654"
     CDS             complement(11418..12113)
                     /gene="tsaB"
                     /locus_tag="ACHK49_001654"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO9262321.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALVYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(12171..14081)
                     /gene="yoaA"
                     /locus_tag="ACHK49_001655"
     CDS             complement(12171..14081)
                     /gene="yoaA"
                     /locus_tag="ACHK49_001655"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO9262322.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            14213..14557
                     /gene="yoaB"
                     /locus_tag="ACHK49_001656"
     CDS             14213..14557
                     /gene="yoaB"
                     /locus_tag="ACHK49_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO9262323.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            14980..15279
                     /gene="yoaC"
                     /locus_tag="ACHK49_001657"
     CDS             14980..15279
                     /gene="yoaC"
                     /locus_tag="ACHK49_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO9262324.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(15399..15578)
                     /gene="yoaH"
                     /locus_tag="ACHK49_001658"
     CDS             complement(15399..15578)
                     /gene="yoaH"
                     /locus_tag="ACHK49_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO9262325.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            15652..17013
                     /gene="pabB"
                     /locus_tag="ACHK49_001659"
     CDS             15652..17013
                     /gene="pabB"
                     /locus_tag="ACHK49_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO9262326.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            17017..17595
                     /gene="nudL"
                     /locus_tag="ACHK49_001660"
     CDS             17017..17595
                     /gene="nudL"
                     /locus_tag="ACHK49_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO9262327.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            17779..19143
                     /gene="sdaA"
                     /locus_tag="ACHK49_001661"
     CDS             17779..19143
                     /gene="sdaA"
                     /locus_tag="ACHK49_001661"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO9262328.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            19274..20872
                     /gene="pdeD"
                     /locus_tag="ACHK49_001662"
     CDS             19274..20872
                     /gene="pdeD"
                     /locus_tag="ACHK49_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO9262329.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(20876..22432)
                     /gene="yoaE"
                     /locus_tag="ACHK49_001663"
     CDS             complement(20876..22432)
                     /gene="yoaE"
                     /locus_tag="ACHK49_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO9262330.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(22420..22578)
                     /gene="yoaL"
                     /locus_tag="ACHK49_001664"
     CDS             complement(22420..22578)
                     /gene="yoaL"
                     /locus_tag="ACHK49_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO9262331.1"
                     /translation="MDRHRRHFSIRPFNAYLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            22895..23866
                     /gene="manX"
                     /locus_tag="ACHK49_001665"
     CDS             22895..23866
                     /gene="manX"
                     /locus_tag="ACHK49_001665"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO9262332.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAQAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            23929..24729
                     /gene="manY"
                     /locus_tag="ACHK49_001666"
     CDS             23929..24729
                     /gene="manY"
                     /locus_tag="ACHK49_001666"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO9262333.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            24742..25593
                     /gene="manZ"
                     /locus_tag="ACHK49_001667"
     CDS             24742..25593
                     /gene="manZ"
                     /locus_tag="ACHK49_001667"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO9262334.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            25648..26106
                     /gene="yobD"
                     /locus_tag="ACHK49_001668"
     CDS             25648..26106
                     /gene="yobD"
                     /locus_tag="ACHK49_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO9262335.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         6399 a         6786 c         6512 g         6521 t
ORIGIN      
        1 agatggaaaa ccgcgcctta tatccccgcc ctgaaggacg gggttttacg gcgcaccgga
       61 taagaacgca cggtattcac cagatctttt atcacttcag ccgccacttc tggcaccagc
      121 aaagtcatcg gcgtctctgt ttcataatcg acagaaacgc cattgctgtt attggtgacg
      181 gtcacggtat acgttgcttt gcccatgatt catttcccgt tatgaatgac tttccgttgt
      241 tgcgcacctt ccatcaggac ttcaggagcc acgaagaagt caatgttgaa ataagtatcg
      301 tcagtcatgg cttcaatgtt gtgccacttt tctggaggga acaccgcaaa ctgccccgct
      361 tcgataagga tcacctgatc aggctctgca ctgtgttcat cagcgtagcc gagatatttg
      421 accgccccat gcataacgga aaggcgtggg taaacccccg ggcgcgttcc tttatcaaga
      481 tgacgttcga atattccggc aggcgcagtt tgtttattcc agaaaggcgt tgagcgcgta
      541 tgaatataat tctgtgggat ttgcagcatc ctattccctc cttcggggaa tgcgctgaaa
      601 acggtttatt ccagccgttt cagggtacgc ctgataattt gcattttaaa taccatttat
      661 tggttacttt ttagcaccat atcagagaaa aatcagggag gattacaagt ggggagcccg
      721 cgacaacgcg ggctgaatgt atcaggattg cagcgtcgcc agtcgggcgg cgaaacccac
      781 gaacatcaaa ccaatcagtg agttgccaac tttagccagt ttctttttgg tacgtatgta
      841 ctgcgtgaca aaagcaccag aaataatcag gaagctcaaa tagcagaaac tcaccagttc
      901 cagcgtcgtc gccagaataa agaatgaaat tcccgtatgt ggggcattaa catcgataaa
      961 ctgtacgaaa aacgacacat agaacaaaat ggctttcgga ttagtcaggc tcaaaattaa
     1021 cgcgcgttta aaaatggccc cgtattgggg ctcatcggat ttggcctcgt tatttttacc
     1081 cttcagggtc gcgtagagaa ttttactccc cagatagagc aaataaaacg caccaagata
     1141 acgtacaatg ttgaataata tcggggtggt cttaattaat gtcgccactc cagcccatgc
     1201 cagaaacatc aatactgcat cgccaataaa tacaccgcag gccgcaagat aaccgccttt
     1261 cataccgcta ctgacgctat ttttgagtac aaacagggta tttggccctg gcaccaacac
     1321 aataaaaatg gccccaacca gataggtcca gtaattcaga accccgtatt cagcgaacac
     1381 attaacctct ttaattatct ttcgatcatg cgcgattaaa ggtgaatatg ctaaccaatc
     1441 tgtagcggct tagaaaggag aaaatcaggt tttaacccga taccaacccg ataattgaat
     1501 cattaatcag gcatgctcca gtgaaaaatt cgggtagtgc tcggcaaaat actggcgtaa
     1561 aaattctacc gttatccgca ctttcgctga cgtcgccagc cttgaaacat aaacggccca
     1621 gacgttcgct ggctggtaat attccggtag cacttgcact aaatgaccac tggcaatgtt
     1681 ttcgctaaca tcccaccagg agcgcagagc aatcccctgc ccatcgagac accactgatg
     1741 gacaatttct ccatgattag acgacagtgg gccggtgact ttaatcgcat ggtggccttc
     1801 tttattgcgt aattgccaga caccaaaagg atgatcgcgc tctttaatga ccaggcacgg
     1861 caatgcggaa agatcggtta aatgtttcgg cgcgccatgt tgcgcaataa attccggcga
     1921 cgcgcagaga atgcggtaat tagtcgccag tttgcgggcg attaaattag gggcgatgtc
     1981 atcgccaatg cgaatatcga gatcaacacc ttcattgacc aaatcgacca accggtcttc
     2041 cacatcaaaa cgtaattcca gttgcggata cgccttagcc agcgctgata acgccggagc
     2101 caccacctgt cgtccaaaac caaagctgct gataatacgc agcatcccct gcggcacctg
     2161 acgcacgtca gaaagttcgt ccatcatctg accgacatcc tgcaaaatcc gctgcgccca
     2221 ttcataaatc cgctctcctt cttcggtaat ggtgacgcgg cgggtggtgc ggtgtaacaa
     2281 caccacgttt agcgtttgct ccagcaaggc gatgcgcttg ctgacgaacg ccggtgaaac
     2341 gcccagttct tccgccacgg cggcaaaacc agcccgacga gccaccagca taaagacgcg
     2401 caaatcattc agcagcggta aattattcat gattcgtgtt ttatgtttca ccagttacgg
     2461 ggattaattc cttttcagtc aattatagga tggtgatgtt gtcaattttg atggtcagga
     2521 agtgagaacc caatgatgaa aacgatgcgt attgctgcga tcccgggaga cgggattggc
     2581 aaagaagtcc ttcctgaagg gattcgcgtg ttacaggctg ccgctgagcg ctggggcttc
     2641 gccttgagtt ttgagcaaat ggagtgggcg agctgcgagt attacagcca tcacggtaaa
     2701 atgatgccgg acgactggca tgagcaactt agccgtttcg acgccatcta ttttggtgcc
     2761 gtcggctggc cggacaccgt tcctgaccat atttcgttgt ggggttcgct gctgaaattt
     2821 cgtcgtgaat tcgaccagta cgtcaacctg cgcccggttc gtctctttcc tggcgttccc
     2881 tgcccgctgg cgggaaaaca gcctggcgac atcgattttt acgtggtcag ggaaaacacc
     2941 gaaggcgaat attcctcgct cggcggtaga gtgaatgaag gtacagagca tgaagtcgtc
     3001 attcaggaat cggtatttac gcgtcgtggt gtcgatcgca ttttgcgtta tgccttcgaa
     3061 cttgcgcaaa gccgcccacg taagacgcta acttctgcca ctaaatcaaa cggtttagcc
     3121 atcagcatgc cgtactggga tgagcgagtg gaagcaatgg ccgagaatta cccggagatc
     3181 cgctgggaca agcagcatat tgatattctc tgcgcgcgtt ttgtgatgca gccggaacgc
     3241 ttcgatgtgg tggtggcgtc caatttattt ggcgatatcc tttccgatct tggcccggcc
     3301 tgcaccggca ccattggcat tgccccatcc gccaacctga atccggaacg cactttcccg
     3361 tcgctcttcg agccagtcca cggttccgcg ccggatatct acgggaaaaa cattgctaac
     3421 cctatcgcca cgatttgggc cggtgcaatg atgctcgatt ttctcggcaa tggcgatgag
     3481 cgtttccagc aagcgcataa cggtattctg gcagcaattg aagaagtgat tgctcacggg
     3541 ccgaaaacac ctgatatgaa aggcaatgcc accacgccac aggttgccga cgcgatttgc
     3601 aaaattattt tgcgttaagg tcaaaccagt ttatttgaac cgcgtcactg acgcggtttt
     3661 tttattcgtt ctttgcagta aataacctgc gtcatttcac cttttattgt ttccgtttcg
     3721 tgttttatgg ctttccgtat tcttaattgt ttaatttatg taacatgcaa ataatcaatt
     3781 tgttaaacac aacatacaac tcattaccct gcaaataatt acaactggca aaagccagag
     3841 acgggctttt atgcccagga gatgactcat gatgagcaat gttaagaaaa aagatgtgcc
     3901 gctgataagc atcagcctgg tggccattct tttcatcgca gctgcattaa gccttttccc
     3961 acaacaatcg gccgacgcgg ccaacgcaat atacactttt gttacgcgta cgttaggttc
     4021 cgccgtacag gtattggttt tgctggcaat gggactggtg atttatttag ccaccagtaa
     4081 atacggcaat attcgtcttg gcgaaggaaa accggaatac agcacgctct cctggctgtt
     4141 tatgtttatt tgtgccggtt taggttcttc tacgctttat tggggggttg ctgaatgggc
     4201 ctattattat caaacgcctg gattaaatat cgcaccgcgt tcacaacagg cactcgaatt
     4261 tagcgtccct tactccttct tccactgggg catcagcgcc tgggcaactt atacgctggc
     4321 ctcattaatc atggcttatc actttcatgt gcgtaaaaac aaaggtctga gtctttccgg
     4381 cattattgcc gcgattaccg gcgttcgccc gcaaggccca tggggaaaac tggttgattt
     4441 gatgttcctg atcgccactg tcggcgcact gactatttcc cttgttgtca ccgcagcaac
     4501 ctttactcgt ggactttccg cgctgaccgg tttacccgat aacttcaccg tgcaggcatt
     4561 tgtgatcctg ctttccggcg gcattttttg cctaagctcg tggattggta tcaacaacgg
     4621 tttgcaacgt ctgagcaaaa tggttggctg gggcgcgttc ctgctgccat tactggtgct
     4681 gattgtcggc ccaaccgaat ttattaccaa cagcatcatc aatgccatcg gtctgaccac
     4741 gcaaaacttc ctgcaaatga gcttattcac cgatccgctt ggcgatggtt catttacccg
     4801 caactggacc gttttctact ggctgtggtg gatctcatac acccctggcg tagcaatgtt
     4861 tgtcacccgc gtttcccgcg gtcgtaagat taaagaagtt atctggggac tgatcctcgg
     4921 cagcaccgtc ggttgctggt tcttcttcgg cgtaatggaa agctatgcca ttcatcagtt
     4981 tatcaatggc gtaatcaacg tcccacaggt gctggaaaca ctgggcggcg aaacagctgt
     5041 acagcaagtt ctgatgtcgt tgccagccgg taaattgttc ctcgccgcat acctgggcgt
     5101 gatgattatt ttccttgcct cgcatatgga tgcggtggcc tacaccatgg ctgcgaccag
     5161 tacgcgtaat ctccaggaag gtgacgatcc tgaccgtggg ctgcgtcttt tctggtgcgt
     5221 ggtgatcact ctgatcccgc tttccatctt gtttaccggt gcttcgctgg aaacgatgaa
     5281 aaccaccgtc gtgctcacag cccttccctt cctcgtcatt ttactggtga aagtcggcgg
     5341 atttattcgc tggctgaaac aggattacgc cgacattccg gctcatcaag ttgaacatta
     5401 tctcccgcag acaccggttg aagccctgga aaaaacgcca gtgctccctg cgggaaccgt
     5461 attcaaaggc gacaactgag cgccatcatc ctaacgataa aggtatccct atgagcaatc
     5521 tgagccctga ctttgtacta cccgaaaatt tttgcgctaa cccgcaagag gcgtggacca
     5581 ttcctgcccg tttttatacc gatcagaacg cgtttgaaca cgaaaaagag aacgtcttcg
     5641 ccaaaagctg gatttgcgtc gctcacagca ttgaactggc gaatgccaat gattatgtga
     5701 cgcgtgagat cattggcgaa agcatcgtgc tggtacgcgg tcgtgataag gttttgcgcg
     5761 cgttctataa cgtgtgtccg caccgtggtc atcagttgtt gagcggtgaa ggaaaagcaa
     5821 aaaatgtgat tacctgcccg tatcacgcat gggcattcaa actcgatggc aatctggccc
     5881 atgcacgtaa ctgcgaaaac gtcgccaatt tcgatagcga caaagcgcaa ctggttccgg
     5941 tgcgtctgga agaatatgcc ggattcgtct tcatcaacat ggaccccaac gccaccagcg
     6001 tagaagatca gttacccggt ctgggcgcga aagtgctgga agcctgcccg gaagtccacg
     6061 atctgaaact ggcggcccgc tttaccaccc gcacgcctgc caactggaag aacattgtcg
     6121 ataactatct cgagtgctat cactgtggtc cggcgcatcc aggtttctcc gactccgtac
     6181 aggttgatcg ttactggcac accatgcacg gtaactggac gctgcaatac ggtttcgcca
     6241 aaccgtccga acagtcgttt aaatttgaag agggtacgga tgcggcattc cacggtttct
     6301 ggctgtggcc gtgcacgatg ctgaacgtca ccccgatcaa agggatgatg acggtcattt
     6361 atgaattccc ggtggattct gaaactaccc tgcaaaacta cgatatttac ttcaccaatg
     6421 aagagttaac cgacgagcaa aaatcgctga ttgagtggta tcgcgatgtg ttccgtccgg
     6481 aagatttacg tctggttgaa agcgtacaga aagggctgaa atcgcgtggc tatcgtggtc
     6541 aggggcgcat catggccgac agtagcggta gtggcatttc cgaacatggt atcgcccatt
     6601 tccataatct gctggcgcag gtgtttaagg actaatgaca tcggcggcgg tattttccgc
     6661 cgctgggctg atttttgatg gagtacagca atgtcagact atcaaatgtt tgaagtacag
     6721 gtgagtcagg tggaaccgct taccgaacag gtgaaacgct tcacgctggt ggcaaccgat
     6781 ggcaaaccgc tgcctgcgtt caccggagga agtcacatca ttgtgcagat gagcgatggt
     6841 gataaccagt acagcaatgc gtattcacta ctgagttcgc cgcatgacac ctcttgttat
     6901 cagattgccg ttcggctgga ggaaaactcg cgcggcggtt cccgcttttt gcatcagcag
     6961 gtaaaagtgg gcgatcggtt aacgatttca acgcctaata acctgtttgc gctaattccc
     7021 tcagccagaa agcatctgtt tatcgcgggc ggtattggta tcaccccttt cctgtcgcac
     7081 atggcagagc tgcaacacag cgacgtcgac tggcagctac attactgctc gcgaaatcca
     7141 gaaagttgcg catttcgtga tgagctagtc cagcatccgc aggctgagaa agttcacttg
     7201 catcactcat caaccggaac acgactggaa ttagcgcgat tattggcgga tatcgaacct
     7261 ggcacacacg tttatacctg tggtcccgag gcgctaaatg aagcggtaag aagtgaagct
     7321 gcgcgtctgg acatcgtcgc cgatacgctg cactttgagc aatttgctat cgaagacaaa
     7381 accggcgatg cctttaccct ggtgcttgcc cgttccggaa aagagtttgt ggtgccggaa
     7441 gagatgacta ttttgcaggt tattgaaaat aataaagccg cgaaagtgga atgtttatgt
     7501 cgtgaagggg tatgcggaac ctgcgaaaca gcaatactgg aaggtgaagc tgaccatcgg
     7561 gatcagtatt ttagcgatga agagcgtgcc agccagcaaa gtatgttgat ctgttgttcg
     7621 cgtgcgaagg gtaaacgcct ggtgttggat ttgtagtttg ccaaagccgg atgtggcgct
     7681 gagcgcgccc agtccggctt cggaagattt tactgcggat attcctgcaa taaattgtgt
     7741 aatgcttccg ccatcagctc accacgccag ccggaaatca gcgccggcaa attgttctgc
     7801 ggtttcagtt tccagtgcca gttcagcagt tggttgattt gccgacgcga tgccagcaat
     7861 tcggcgctga tcttatgcgt ttcgctcacg tcagtaatca gcgacttaat cgctttaaac
     7921 actttacgat aaccaggcat gtccatcagg ttaagcatcg gctgcggtaa ggcctcttcc
     7981 ggcaatgtct gcgctttttc caccagcgcc agcagcgttt taccgtgaaa gcggatttcg
     8041 ctaccggata aacccaggct gtccagttcg cctaaacttc ccggcatata acgcgcgacc
     8101 gaccacaaat gctcttcacg cacgacaaag ttcaccgcca gatcacgctc tcgcgccttg
     8161 cgcagtcgcc agtcggctaa cagttgcaga caggccagtt ggcgtgtgcg taactgccag
     8221 gcattggtga tatcacgcca ggcatcttcc ggcgcaacga cttcctgacg acgcatttgc
     8281 atcaggcggc attcatccag cgccgcaggt agccagccgg aggcctccgt ttctaccata
     8341 agcttggcgg tgatcggtaa cagataccag acatccgccg ctgcgtattc acactgacgt
     8401 tcggtcagcg gtctggccag ccagtcggtg cgcgattcac tcttgtccag cgtaacgccg
     8461 gaatactctt ccaccatgga agcgaaaccc catgacatcg ggcgtccgca gaaggcagca
     8521 aggatttgcg tgtcaatcaa gggttgtggt aattcgccaa agacattgag gaacacttcc
     8581 agatcttcac tgcctgcatg gagaaatttt gtgatggacg gatcgcgcag gatcgctttc
     8641 agcggtgacc agtcggtgat cccgagagga tcaattagcg ccagatgctc gccatcgaaa
     8701 agttgaatca accccagctg cgggtaataa gtgcgcgtac gaacaaattc agtatccagg
     8761 gctatcgccg gaaaggcacg gacggcttca cacaaagaag ccagcgcatc gtccgtggta
     8821 atcatttggt aattcaaatt gttttctctt tagtgggcgt caaaaaaaac gccggattaa
     8881 ccggcgtctg acgactgact taacactcag gctttattgt ccactttgcc gcgcgcttcg
     8941 tcacgtaatt ctcgtcgcaa aatttttccg acgttagatt tcggtaactc atcacgaaac
     9001 tccaccagct tcggtacttt gtagcccgtg agctgacggc ggcaaaaggt caccagtgac
     9061 tcttcggtaa gcgatggatc ttttttcact acgaagattt tcaccgcttc accactggag
     9121 ccggaaggta cgccaacagc cgcgacttcc tgtacgccag gatgctgcat gacgacatct
     9181 tcaatctcgt tgggatagac gttaaaaccg gaaaccagaa tcatgtcttt tttacgatcg
     9241 acaatgcgca ggaacccttc ttcatccatc accgcgatgt cgccggtgtg taaccagcca
     9301 tttttgatga tctcatctgt agcatccgga cgctgccagt aacccagcat cacctgcggt
     9361 cctttgacac aaagctcacc cggttgaccc ggtggtactt cattatcatc atcatccacc
     9421 agtttggctt ccgtcgacgg caccggcaaa ccgatgctac cactatgata atcaatatca
     9481 tatgggttaa cgctgaccag cggcgcacac tcggtaaggc catagccttc cagcagatac
     9541 tgtcctgtca gtttcaccca acgctctgcc accacttgct gcactggcat ccctccgcct
     9601 gcggaaagat gcagactgga gaaatccagc tgctggaact ctttattgtt cagcaacgca
     9661 ttgaacaagg tgttaacgcc cgtgatagcg gtaaacggat atttcgctaa ctcttttacc
     9721 aaccctggaa tatcgcgcgg gttagtgata agcaggttct gcccacccag ttcgataaac
     9781 agcaggcagt taatggtcag ggcaaaaatg tgatacagcg gcagcgccgt caccaccagc
     9841 tctttgcccg gatgcaacag cggaccatag gtcgcgttaa cctgttccag gttcgccagc
     9901 atattgcggt gagtcagcat cgcgcctttc gccacaccag tggtgccgcc ggtgtattgc
     9961 agaaaagcta aatcttccgg caccagttcg ggtttgacgt actgcatccg gtagccgtta
    10021 tgcagtgcgc tacgaaatga aatggcatct ggcagatggt atttcggcac caaacgcttg
    10081 atgtatttaa caacgaaatt gactaccgtg ccttttgccg tagatagctg atcgcccata
    10141 cgggtcagaa ttacgtgctg aacggcggtt ttatcaacca ctttttccag tgtgtgagca
    10201 aagttagaca cgataacaat cgccgatgcg ccgctatcgt taagctgatg ctcaagctca
    10261 cgcggggtat acaacgggtt aacgtttacg acgatcatcc cggcacgcaa aatgccaaac
    10321 agcgccaccg gatattgcaa taaattaggc atcatcaacg caacgcgatc gcctttcttc
    10381 agccccaacc cttgttgcaa ataagcggca aacgcgcgac tgcgttcttc cagcttgcgg
    10441 aaggtcatta cctcccccat attcacaaac gcaggttgat cggcgtagcg cgcgaccgac
    10501 tgctcaaaca tatctaccag agattgataa cggtcagggt tgatctccgt cggaacgtcc
    10561 gcgggataac ggttaagcca aaccttcttc aattcttcac ctctaaaatg cgtgttcgtc
    10621 gtcatcgcaa ccccaaatga tatacatgcc gttaacataa tattaactca tcataccagc
    10681 ttgataatta cccaacgaaa aggttgcgaa gcgcgtcact atttattttt atctttaccg
    10741 taagaatgca gaaacagcgg accagccgct gtttcttttt ctttaaaaac aagcaattat
    10801 tcagttacaa ctgtttgtac tctcgcgggg ccgggattat accagcccca tccgccgtag
    10861 ccatagggcc agccacgacc gccataaaac catggatcaa tcggctgagg cggcataatc
    10921 acctgctggg ttaaatgcca acgtttgtaa cccgttactt gcatcaccat aaatttatag
    10981 ggcgtattgc cgattttgcc gtcaaccgca ccagtgattg gcccgactac cgtaacaagt
    11041 tgtccacgga aatccaccgg gtccagaaaa ccgttcacat cggcataaat gcgaccgcga
    11101 gaaggttctc ccagcgtcgg tctggctccg ctgtccagcg gtacggtagc aatttccagg
    11161 cgggttttcc cttgctggtt ttgtaccgca accactttgc caccaaagcg tgcctcctga
    11221 ccaacgtaca gctgcggcgc actcatcacc cgaactaaat cttgttgcgg cgtgggactg
    11281 ctgcctttaa tggcgtccgg cacagtgaca caaccgctca gcattagcgc aaacgtacct
    11341 gccagtatgc ctttgataac attcttttga accgccatga tgcgactcct ttttttctca
    11401 gggcatactc ttaagattca ttctttgccc ggaagtttct tccatgcgac gttgttacgt
    11461 aaataaaccg gttcggcatg ttccaccgcc accgttttac cctcggcaaa catctgacac
    11521 gcaatcggca gcatatcttc cgccgctggc agtaaaactt ctccgtcgcg caaaaccagc
    11581 ccgctctctt taccgagatc cggccaggct tgccagcccg tccctaccgt tacccattcg
    11641 ccggaaagct gttgcattcg ttcatgaacg agttcgggtt tgagtacggc ttcggtttct
    11701 tcaccgcgcc agataccgtt ttcatcacgc tgatattcgg cccagtaaac ttcgcccatt
    11761 cgcgcgtcaa tggctgccag cacgcgggtt gcgccgtttt tgcgccacgc cccttgcgcc
    11821 atcgtcatta gcgtggagac gccaatcatc ggtagctctg cgccaagtgc cagcccttgc
    11881 gcaatgccaa taccaatgcg cacgccggta aagctaccgg ggccgcgccc gtaaaccaga
    11941 gcgttaatat cagtcaggga agttccgctg gtggtcagga tatcctgcac catcggtaag
    12001 attcgttgag tatgttcacg agggcaaagc tcaaaatgag cgttgacagt accgtcgttc
    12061 cacagggcga cagagcaggc ctctgtcgcg gtatcgatag ccagaattcg catgggtctt
    12121 cgtgcttaga tcaataaaaa ggcgcgcatc ataccatact ccgtaacaaa ttacctggag
    12181 gatggtatcg caaggaaacg aaccgcacgg gcaatgtcac gagtgcgtgg cgcgggcggc
    12241 agactggcga gaaacgtcgc gccgtaagga cgcatcacca gccgattgtc acaaatcacc
    12301 agcacgccac gatcgtcggc gtcgcgaatc agtcgcccta ccccctgttt gagagtaatg
    12361 acggcatctg gtagttgcac ttcatcgaac gggtcgccac cgcgcaaacg acaatcttcc
    12421 atgcgcgctt ttaacagtgg atcatccggc gaggtaaacg gcaacttgtc gataatcacc
    12481 aatgacaatg tatcgccacg cacgtccacc ccttcccaga aactactggt tgccacaaga
    12541 agcgcgttac cggcgctgac aaattgctgc aacagttgcc ctttgctggt ttccccctgc
    12601 aacaaaacgg gaagcgtcat ggtagcgcgg aactgctcgg ccagatcacg catcatggcg
    12661 tgcgaggtac aaagcataaa acaacgaccg ttgttagctt cgatgatcgg tcgcagcatt
    12721 gccgctaact ggcgagcaga acctggctgg ttggtttgcg gcagattgcg cggcacacag
    12781 agtaacgcct ggcggctgta atcaaacggg cttggcaata gcaacgactc cgcctgttct
    12841 atcccaagcc gcgaggtgaa atgatgcaga tcgtcgttca ccgacagcgt tgctgaggtg
    12901 aagatccagc taccaggctt ttgcgccatt aactctttga atttatccgc cacgctgagc
    12961 ggcgtgagag ccagagtaaa atggcgcgaa gtgcattcgt accagtagct gtagcccggc
    13021 tgattgatct cttttagccg cttcagccgt gtgcgataca acgtagcgcg ctcaaatgcc
    13081 gcatccagca aggcggaacg ccccagtgac agtttcgcca cgtcataaca aagttccagg
    13141 gtgtcatcga gcagtaaaaa tgcccgctga atttgcggat tagctaacag ctcacgcagg
    13201 ttaccgcgat aacctggctc accgagttgc agacgaaaat cctgcgcact ctgggcaaga
    13261 cgatcagcgc acttttgtaa ctgctgggtg tcttttaatt cggtgcggta agcgatggtg
    13321 atgtcttttg ccaggtcgag cagttgtcga ctggagagtg actgaccaaa atactggctg
    13381 gcaatgtccg gtagctggtg ggcttcgtcg aagatcatga cgtccgcttc cgggatcagt
    13441 tcgccaaatc cactctcttt aaccaccata tccgccagaa agagatgatg gtttaccacc
    13501 accacatcgg cgtccatcgc ttttttacgt gctttgacca caaagcaatc tttatacatc
    13561 gggcagtcgc tgccaagaca gttgtcgttg gtgctggtga ccagcggcca cgcctgtgaa
    13621 tcttccgcca cgctgacgca ggtgctgata tcaccatcga ctgtttgatt agaccaggag
    13681 cgcagcagga tcacatcgct taagatttgt accggcagat cgccccccgc cagcgcctgc
    13741 tgttcgagac gctcgaggca gaggtagttt gagcgccctt tcagcagcgc cacgttgccc
    13801 gtatatttca atgcctttga gactgttggc aaatcgcggc tgtagagctg atcctgcaac
    13861 gcttttgagc cggtcgagat aatgactttc tttttcgccc gcagcgcagg agccaggtaa
    13921 gcgtaggttt tgcccgtacc ggttcctgct tccaccacca gcggctggcc tttttctatc
    13981 gcctgggtga cggctaccgc catctgtcgc tgtggttctc gcggcttaaa gcctggtatc
    14041 gctttcgcca gctgaccgtc tggtgcaaaa tcgtccgtca cactaccccc tgttgatttg
    14101 aacagggatt atgtcaggat gagggcgctt tcgccagttg aattggtgac ggcgacctca
    14161 cattgtggca gtctttgcag cacgaaaatg gaaggataat gaggacaaaa tgatgactat
    14221 cgttcgtatc gatgctgaag cccgctggtc tgatgtggta atccacaaca acacgctcta
    14281 ctacactggt gtaccggaaa acctcgacgc cgatgccttt gagcaaaccg ccaacacgct
    14341 ggcgcagatt gatgccgtgc tggaaaaaca gggcagcaat aaatcgagca ttctggatgc
    14401 caccattttc ctggccgata aaaacgactt cgcggcgatg aataaagcgt gggatgcctg
    14461 ggttgtcgcg ggtcatgcgc cggtgcgctg cacggtacaa gcgggtttga tgaacccgaa
    14521 gtataaagtt gaaattaaga ttgtggctgc ggtgtaagct ttatcgaagc aaaataagtc
    14581 agacgataat ttatcgataa tactggtcgg ttttacataa atcgaccaga gattaagata
    14641 ttacctgttg atgctcctgc taattcatta tttattttta taatttcaat tttatctata
    14701 aaactaacta tataaataac cataaccatg ccgttgtgtt cgccctctga ctagcaactc
    14761 tggttttctt tcttagctga ataatctggt tatagcgtga ggactgaccg ttaaatttct
    14821 ccatcttact ggataacaca ccatgagctg ctacctttaa aacacctgaa tgtcaacagg
    14881 ttaactcgtg ccatatcgtt ataatccctt tgatattaga tgcaaattaa ggtcatatat
    14941 agtcttatcg ataacatggt taattttaag gataagaata tgcctgctgt aatagataaa
    15001 gccctggatt tcattggtgc catggatgta tcagcgccaa caccaagttc gatgaatgaa
    15061 agcacggcga agggaatctt taaatattta aaagaactgg gagtacccgc gagtgccgct
    15121 gatattacgg cgcgagctga ccaggaaggc tggaaccctg ggttcacgga aaaaatggtt
    15181 ggatgggcaa aaaaaatgga gacaggtgaa cgctctgtga ttaaaaatcc tgaatacttt
    15241 tcaacatata tgcaggaaga actaaaagca ctggtctgag ttaaatttat atcagcataa
    15301 atgggtcagg acgcttttaa tcacatataa aaagcgtcct ttttctcaca atcaaatgat
    15361 catcggcttc tataacgaaa tcgatacgga ttagtgtgtt actcgtcttc atcctcaaaa
    15421 cgcgccacga ttcgctcgcc ggagtggttg gcacgcagct cttctgccac cagcgcgatt
    15481 gcctggccgc tgctcatccc ttgtgccatc agttcctgga tccgctcgac agctttttgc
    15541 tgctgttcat gggtgagtga aggtaaacct gcaaacattg ttaactcctg ctaaattgtt
    15601 ggcgctaatt atttcatgct acccggcaca tagccagtag agtcaggact gatgaagacg
    15661 ttatctcccg ctgtgattac tttaccctgg cgtcaggacg ccgctgaatt ttatttctcc
    15721 cgcttaagcc acctgccgtg ggcgatgctt ttacactccg gctatgccga tcatccgtat
    15781 agccgctttg atattgtggt cgccgatccg atttgcactt taaccacttt cggtaaagaa
    15841 accgttgtta gtgaaagcga aaaacgcaca acgaccactg atgacccgct acaggtgctc
    15901 cagcaggtgc tggatcgcgc agacattcgc ccaacgcata acgaagattt gccatttcag
    15961 ggcggcgcac tggggttgtt tggctacgat ctgggccgcc gttttgagtc actgccagaa
    16021 attgcggaac aagatatcgt tctgccggat atggcagtgg gtatctacga ttgggcgctc
    16081 attgtcgacc accagcgtca tacagtttct ttgctgagtc ataatgatgt caatgcccgt
    16141 cgggcctggc tggaaagcca gcaattctcg ccgcaggaag atttcacgct cacttccgac
    16201 tggcaatcca atatgacccg cgagcagtac ggcgaaaaat ttcgccaggt acaggaatat
    16261 ctgcacagcg gtgattgcta tcaggtgaat ctcgcccagc gttttcatgc gacctattct
    16321 ggcgatgaat ggcaggcatt ccttcagctt aatcaggcca accgcgcgcc atttagcgct
    16381 tttttacgtc ttgaacaggg tgcaatttta agcctttcgc cagagcggtt tattctttgt
    16441 gataatagtg aaatccagac ccgcccgatt aaaggcacgc taccacgcct gcccgatcct
    16501 caggaagata gcaaacaagc agaaaaactg gcgaactcag cgaaagatcg tgccgaaaat
    16561 ctgatgattg tcgatttaat gcgtaatgat atcggtcgtg ttgccgtagc cggttcggta
    16621 aaagtaccag agctcttcgt ggtggaaccc ttccctgccg tgcatcatct ggtcagcact
    16681 ataacggcgc gactaccaga acagttacac gccagcgatc tgctgcgcgc agcttttcct
    16741 ggtggctcaa taaccggggc tccgaaagta cgggctatgg aaattatcga cgaactggaa
    16801 ccgcagcgac gtaatgcctg gtgcggcagc attggctatt tgagcttttg cggcaacatg
    16861 gataccagca ttactatccg cacgctgact gccattaacg gacaaatata ctgctctgcg
    16921 ggcggtggaa ttgtcgccga tagccaggaa gaagcggaat atcaggaaac ttttgataaa
    16981 gttaataaga tattacgcca actggagaag taagacgtgg aataccgtag cctgacgctt
    17041 gatgattttt tatctcgctt tcaacttttg cgcccgcaaa ttaaccggga aaccctaaat
    17101 catcgtcagg ctgccgtgtt aatccccatc gtccgtcgac cgcaaccggg gttgttgctg
    17161 actcagcgtt caattcatct gcgtaaacac gctggacaag tggcattccc tggaggtgca
    17221 gtcgatgaca cggacgcatc agttatcgcc gccgcgctgc gcgaagctga agaagaggtc
    17281 gctataccgc cttccgccgt tgaagttatc ggcgtgctgc cgcccgtcga tagcgtaact
    17341 ggctatcagg taaccccagt ggtcggcatt atcccgcccg atctgccgta tcgcgccagt
    17401 gaagatgaag tctcggcggt gtttgaaatg ccgctcgccc aggcattaca tctgggtcgt
    17461 tatcaccctt tagatatcta ccgccgtggc gattcacatc gggtatggct gtcctggtac
    17521 gaacagtatt ttgtatgggg aatgaccgca ggcataattc gtgagctggc gctgcaaatt
    17581 ggtgtgaaac cctgactata cttatcttta catctacaaa acactacttg agacaatcat
    17641 cgcaatatta gttaaatcgc ggtttttgat tagtttaatt catgtgaata gttaagccag
    17701 tcgccgcgtt ccctcttaca ctatgcgctg ttattagttc gttactggaa gtccagtcac
    17761 cttgtcagga gtattatcgt gattagtcta ttcgacatgt ttaaggtggg gattggtccc
    17821 tcatcttccc ataccgtagg gcctatgaag gcgggtaaac agttcgtcga tgatctggtc
    17881 gaaaaaggct tactggatag cgttactcgc gttgccgtgg acgtttatgg ttcactgtcg
    17941 ctgacgggta aaggccacca caccgatatc gccattatta tgggtcttgc aggtaacgaa
    18001 cctgccaccg tggatatcga cagtattccc ggttttattc gcgacgtaga agagcgcgaa
    18061 cgtctgctgc tggcacaggg acggcatgaa gtggatttcc cgcgcgacaa cgggatgcgt
    18121 tttcataacg gcaacctgcc gctgcatgaa aacggtatgc aaatccacgc ctataacggc
    18181 gatgaagtcg tctacagcaa aacttattat tccatcggcg gcggttttat cgtcgatgaa
    18241 gaacactttg gtcaggatgc tgccaacgaa gtgagcgtgc cgtatccgtt caaatctgcc
    18301 accgaactgc tcgcgtactg taatgaaacc ggctattcgc tgtctggtct cgctatgcag
    18361 aacgaactgg cgctgcacag caagaaagag atcgacgagt atttcgcgca tgtctggcaa
    18421 accatgcagg catgtatcga tcgcgggatg aacaccgaag gtgtactgcc aggcccgctg
    18481 cgcgtgccac gtcgtgcgtc tgccctgcgc cggatgttag tttccagcga taaactgtct
    18541 aacgatccga tgaatgtcat tgactgggta aacatgtttg cgctggcagt taacgaagaa
    18601 aacgccgccg gtggtcgtgt ggtaactgcg ccaaccaacg gtgcctgcgg tatcgttccg
    18661 gcagtgctgg cttactatga ccactttatt gaatcggtca gcccggacat ctatacccgt
    18721 tactttatgg cagcgggcgc gattggtgca ttgtataaaa tgaacgcctc tatttccggt
    18781 gcggaagttg gttgccaggg cgaagtgggt gttgcctgtt caatggctgc tgcgggtctt
    18841 gcagaactgc tgggcggtag cccggaacag gtttgcgtgg cggcggaaat tggcatggaa
    18901 cacaaccttg gtttaacctg cgacccggtt gcagggcagg ttcaggtgcc gtgcattgag
    18961 cgtaatgcca ttgcctctgt gaaggcgatt aacgccgcac ggatggctct gcgccgcacc
    19021 agtgcaccgc gcgtctcgct ggataaggtc atcgaaacga tgtacgaaac cggtaaggac
    19081 atgaacgcca aataccgcga aacctcacgc ggtggtctgg caatcaaagt ccagtgtgac
    19141 taatacttct tactcgccca tctgcaacgg atgggcgaat ttatacccgc tttctcgtct
    19201 gctgtaatat tccccactac acttccactg ttgcgtcagg cgtttgtcgc catacgctta
    19261 cagggtggcc cgcatgcaaa aagcacaacg gatcattaaa acctatcgcc gtaatcgaat
    19321 gattgtttgt acgatttgcg cactcgttac gctcgcttcg accctgagcg tgcgatttat
    19381 ttcacagcgt aacttaaatc aacaacgggt agtacaattc gccaatcacg ctgtagagga
    19441 attagataaa gtactgcttc ccctacaggc aggtagcgaa gtcttgcttc cgctgattgg
    19501 tctgccctgc tctgtcgccc atttgccatt acgtaaacag gcggcaaaac tccaaactgt
    19561 gcgatccatt ggcctggtgc aagacggcac actctattgc tccagcattt ttggttatcg
    19621 caatgtgccc gtcgtggaca ttctggctga acttcctgca ccgcaaccac ttttacgcct
    19681 gacgatcgac cgtgccctga ttaaaggcag tccggttttg attcaatgga cgcctgcagc
    19741 gggcagtagc aatgctgggg tcatggagat gattaacatc gacttactga cggcaatgct
    19801 ccttgagcca caactgccgc aaatcagtag cgccagcctg acggtggaca aacggcattt
    19861 gctctatggt aatgggctgg tagattccct tccgcaacct gaagacaatg aaaactatca
    19921 ggtttcttcg caacgctttc cttttaccat taacgttaat ggtccggggg ctacggcgct
    19981 ggcatggcac tatcttccaa cacaattacc gctggcggtg ctgctaagtt tactggtggg
    20041 ctacatcgcc tggctggcga ccgcttaccg gatgagcttt tcccgcgaaa tcaatctggg
    20101 cctggcgcaa catgagttcg aattgttctg tcagcctttg cttaatgcgc gcagccagca
    20161 atgtattggt gtagagattt tgctgcgctg gaataatccg cgtcagggct ggatttcacc
    20221 ggatgtgttt attcctatcg cggaagaaca tcatttaatt gtgccactga cccgctatgt
    20281 gatggcagaa accattcgtc agcgccatgt tttcccgatg agtagtcagt ttcatgttgg
    20341 cattaacgtc gcacccagcc attttcgccg tggtgtgctg ataaaagatc tcaatcagta
    20401 ctggtttagc gctcacccga ttcagcaact gatcctcgaa atcaccgaac gcgatgcctt
    20461 actggatgtt gattatcgga ttgcccgcga gctacatcgt aaaaacgtca aactggcgat
    20521 tgatgacttc ggcaccggca acagttcatt ttcctggctt gaaacattac gtcctgacgt
    20581 gctgaaaatt gataagtcat ttaccgcagc tataggttct gacgcggtta actcgacggt
    20641 gaccgatatc atcatcgcgc tggggcaaag actgaatatt gaactggtgg cggagggcgt
    20701 ggaaacgcaa gaacaggcga agtatttgcg ccgtcatggc gtgcatattt tgcaagggta
    20761 tttgtacgca cagccgatgc cgctacgtga ttttcccaaa tggctggcgg gcagccaacc
    20821 gccgcccgcc cggcataatg gacatatcac gcccattatg ccgttacgtt aatgcttact
    20881 catcttcatc gtgcgccggt tgctctttaa caatgcgaac cagatcaaca cgataatcat
    20941 tggcttcaat gatggtgata tgcagtggcc ctacatcaat cacatcgccc acacggggaa
    21001 tgtgaccatt tgccgagatc acgaggcccg cgaccgtcgc gatatcatcg tcatcggcaa
    21061 ggtgctcaac atcaagcgcc tgctgcaagg catgcaaatc tgtaccgcct tttaccagcc
    21121 agccgtcacc atcagtaatg atttccggcg tttcgtcagc gtccgggaat tcacccgcaa
    21181 tggcttccag cacatccagc ggcgtgacca gaccttgtac cacaccaaac tcgttggtca
    21241 cgataacaaa gctcccgcga gcacgacgca gcacgcccaa caggttgatc ggatcgaggg
    21301 tttccgggac gataatcgcc ggagacgccg aagcaatcgc cgccacatca acgccctctt
    21361 ccagcgccac cagcagttct ttagcacgta caataccgat gatttcatcc agttcaccgc
    21421 gacataccgg gaacagactg tgcggtgaag agagcagttg ctcgcggatt tcatcgaccc
    21481 cgagattagc gtcaacccag cttatttcac cgcgcggcgt catgatcccg cgcagagaac
    21541 gcgacgccag cgtcagtacg ccgttaatca tgtaacgttc ttcttcggca aatgcacctt
    21601 ccgggatcgg catcggcatc gggttatcgg catcgtgctg aacattggcc tgacgtttcc
    21661 cgcccatcaa acgcaggatg gcatcggcag tacgcgctcg cagcggcaaa gtcgactggt
    21721 ggcgaataaa gttgcgacgc gcaatctggt taaacacttc gatgatgatc gagaagccaa
    21781 tcgcggcata caggtaacct ttcggaatgt ggaaaccgaa accttctgcc accagactca
    21841 gaccaatcat taacaggaag ctcagacaga gcaccaccac cgtggggtgc tggttaacga
    21901 atcgcgtcag cggtttggat gccagcaaca taaccgccat cgcaatcact accgccgcca
    21961 tcatcaccgg cagatggtta accatcccta ctgcagtaat taccgcatcc aacgagaaga
    22021 cggcgtcaag gatgacaatc tgtgtgacga ccacccagaa actggcgtag cctttaccgt
    22081 ggccggaatc atgatcgcgg ttttccagcc gttcatgcag ttcggttgtt gctttgaaca
    22141 gcaagaatat ccccccgaac aacataatca ggtcgcgtcc ggagaaggag aaatccatga
    22201 cggtaaatag cggtttggtc agcgtgacca tccatgaaat cagcgacagc agccccagac
    22261 gcataatcag cgccagtgat aaccccagca aacgcgcttt atcgcgttgt tttggcggca
    22321 gtttgtcagc aagaatggcg atgaagacca ggttatcgat acccagcaca atttcgagaa
    22381 caacaagcgt gagtagcccc gcccaaattg aggggtccat taagaattcc atgacaagct
    22441 cctgcttaag gaatagctat tcgacgccag aaataatgca ggcgtaacga caaaatgcaa
    22501 acgaaaggtg cggcatagag agccagaaag gtaggcgtta aaaggcctga tgctgaaatg
    22561 acgtcggtga cgatccatac tgcgggctac tgccctatac tccatggttg ttaaacggga
    22621 gttaaacata tcagagacgc ctctgatttg gcaaagattt accttccttt gcaaacgaat
    22681 gtgacaagga tattttacct ttcgaaattt ctgctaatcg aaagttaaat tacggatctt
    22741 catcacataa aataattttt tcgatatcta aaataaatcg cgaaacgcag gggtttttgg
    22801 ttgtagccct tatctgaatc gattcgattg tggacgacga ttcaaaaata catctggcac
    22861 gttgaggtgt taacgataat aaaggaggta gcaagtgacc attgctattg ttataggcac
    22921 acatggttgg gctgcagagc agttgcttaa aacggcagaa atgctgttag gcgagcagga
    22981 aaacgtcggc tggatcgatt tcgttccagg tgaaaatgcc gaaacgctga ttgaaaagta
    23041 caacgctcag ttggcaaaac tcgacaccac taaaggcgtg ctgtttctcg ttgatacatg
    23101 gggaggcagc ccgttcaatg ctgccagccg cattgtcgtc gacaaagagc attatgaagt
    23161 cattgcaggc gttaacattc caatgctcgt ggaaacgtta atggcccgtg atgatgaccc
    23221 aagctttgat gaactggtgg cactggcagt agaaacaggc cgtgaaggcg tgaaagctct
    23281 gaaagccaaa ccggttgaaa aagccgcgca agcacccgct gccgcagcac caaaagcggc
    23341 tccaactccg gcaaaaccaa tggggccaaa cgactacatg gttattggcc ttgcgcgtat
    23401 cgacgaccgt ctgattcacg gtcaggtcgc cacccgctgg accaaagaaa ccaatgtctc
    23461 ccgtattatt gttgttagtg atgaagtggc tgcggatacc gttcgtaaga cactgctcac
    23521 ccaggttgca cctccgggcg taacagcaca cgtagttgat gttgccaaaa tgattcgcgt
    23581 ctacaacaac ccgaaatatg ctggcgaacg cgtaatgctg ttatttacca acccaacaga
    23641 tgtagagcgt ctcgttgaag gcggcgtgaa aatcacctct gttaacgtcg gtggtatggc
    23701 attccgtcag ggtaaaaccc aggtgaataa cgcggtttcg gttgatgaaa aagatatcga
    23761 ggcgttcaag aaactgaatg cgcgcggtat tgagctggaa gtccgtaagg tttccaccga
    23821 tccgaaactg aaaatgatgg atctgatcag caaaatcgat aagtaacgta ttgtgttgat
    23881 tatcactcag ttttcacact taagtcttac gtaaacagga gaagtacaat ggagattacc
    23941 actcttcaaa ttgtgctggt atttatcgta gcctgtatcg caggtatggg atcaatcctc
    24001 gatgaatttc agtttcaccg tccgctaatc gcgtgtaccc tggtgggtat cgttcttggg
    24061 gatatgaaaa ccggtattat tatcggtggt acgctggaaa tgatcgcgct gggctggatg
    24121 aacatcggtg ctgcagttgc gcctgacgcc gctctggctt ctatcatttc taccattctg
    24181 gttatcgcag gtcatcagag cattggtgca ggtatcgcac tggcaatccc tctggccgct
    24241 gcgggccagg tactgaccat catcgttcgt actattaccg ttgctttcca gcacgctgcg
    24301 gataaggctg ctgataacgg caacctgaca gcgatttcct ggatccacgt ttcttctctg
    24361 ttcctgcaag caatgcgtgt ggctattccg gccgtcatcg ttgcgctgtc tgttggtacc
    24421 agcgaagtac agaacatgct gaatgcgatt ccggaagtgg tgaccaatgg tctgaatatc
    24481 gccggtggca tgatcgtggt ggttggttat gcgatggtta tcaacatgat gcgtgctggc
    24541 tacctgatgc cgttcttcta cctcggcttc gtaaccgcag cattcaccaa ctttaacctg
    24601 gttgctctgg gtgtgattgg tactgttatg gcagtgctct acatccaact tagcccgaaa
    24661 tacaaccgcg tagccggtgc gcctgctcag gcagctggta acaacgatct cgataacgaa
    24721 ctggactaac aggtgagcga aatggttgat acaactcaaa ctaccaccga gaaaaaactc
    24781 actcaaagtg atattcgtgg cgtcttcctg cgttctaacc tcttccaggg ttcatggaac
    24841 ttcgaacgta tgcaggcact gggtttctgc ttctctatgg taccggcaat tcgtcgcctc
    24901 taccctgaga acaacgaagc tcgtaaacaa gctattcgcc gtcacctgga gttctttaac
    24961 acccagccgt tcgtggctgc gccgattctc ggcgtaaccc tggcgctgga agaacagcgt
    25021 gctaatggcg cagagatcga cgacggtgct atcaacggta tcaaagtcgg tttgatgggg
    25081 ccgctggctg gtgtaggcga cccgatcttc tggggaaccg tacgtccggt atttgcagca
    25141 ctgggtgccg gtatcgcgat gagcggcagc ctgttaggtc cgctgctgtt cttcatcctg
    25201 tttaacctgg tgcgtctggc aacccgttac tacggcgtag cgtatggtta ctccaaaggt
    25261 atcgatatcg ttaaagatat gggtggtggc ttcctgcaaa aactgacgga aggggcgtct
    25321 atcctcggcc tgtttgtcat gggggcattg gttaacaagt ggacacatgt caacatcccg
    25381 ctggttgtct ctcgcattac tgaccagacg ggcaaagaac acgttactac tgtccagact
    25441 attctggacc agttaatgcc aggcctggta ccactgctgc tgacctttgc ttgtatgtgg
    25501 ctactgcgca aaaaagttaa cccgctgtgg atcatcgttg gcttcttcgt catcggtatc
    25561 gctggttacg cttgcggcct gctgggactg taagactgtt gtacactacc ggggcctttt
    25621 ggccccgttt ttttatctgg aggattaatg acaatcacgg acctggtact gattcttttc
    25681 atcgcagcac tcctggcctt cgcgatctac gatcagttca tcatgccccg ccgtaacggc
    25741 cccaccctgc tggcaattcc tttgctccgg cgtggtcgca tcgatagcgt tatcttcgtc
    25801 ggattgattg tcattcttat ctataacaac gtcacgaatc atggtgcgtt aataacgaca
    25861 tggttattaa gcgcactggc tctgatgggt ttttatatat tctggatccg cgttccgaag
    25921 atcatcttta aacaaaaagg ttttttcttc gccaatgtct ggattgaata tagccgaatc
    25981 aaagcgatga acttgtcgga agatggcgtg ttggtgatgc aattagaaca gcgtcggctg
    26041 ttaatccgcg ttcgaaatat cgacgatctg gaaaaaattt ataagcttct cgtttcaact
    26101 caataagtta tgaatttagc caaagctatg tttagtgtat ttttaataat cagacatagc
    26161 ttaggctata ttacctcttc ccttatttgt tatttatttt aacgtttcat tgatatat
//
LOCUS       DAZGLJ010000044        26176 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000044 DAZGLJ010000000
VERSION     DAZGLJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.044"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            370..744
                     /gene="tomB"
                     /locus_tag="ACHK49_001669"
     CDS             370..744
                     /gene="tomB"
                     /locus_tag="ACHK49_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO9262336.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            770..988
                     /gene="hha"
                     /locus_tag="ACHK49_001670"
     CDS             770..988
                     /gene="hha"
                     /locus_tag="ACHK49_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO9262337.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            1160..1711
                     /gene="maa"
                     /locus_tag="ACHK49_001671"
     CDS             1160..1711
                     /gene="maa"
                     /locus_tag="ACHK49_001671"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO9262338.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPVRIIKKL"
     gene            1827..2297
                     /gene="ylaC"
                     /locus_tag="ACHK49_001672"
     CDS             1827..2297
                     /gene="ylaC"
                     /locus_tag="ACHK49_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO9262339.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            2461..4011
                     /gene="pdeB"
                     /locus_tag="ACHK49_001673"
     CDS             2461..4011
                     /gene="pdeB"
                     /locus_tag="ACHK49_001673"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO9262340.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGQLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(4053..4406)
                     /gene="ybaA"
                     /locus_tag="ACHK49_001674"
     CDS             complement(4053..4406)
                     /gene="ybaA"
                     /locus_tag="ACHK49_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO9262341.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(4526..4622)
                     /gene="ffs"
                     /locus_tag="ACHK49_001675"
     ncRNA           complement(4526..4622)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHK49_001675"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4784..5095
                     /locus_tag="ACHK49_001676"
     CDS             4784..5095
                     /locus_tag="ACHK49_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO9262342.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(5126..5698)
                     /gene="ybaY"
                     /locus_tag="ACHK49_001677"
     CDS             complement(5126..5698)
                     /gene="ybaY"
                     /locus_tag="ACHK49_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO9262343.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            5916..6776
                     /gene="tesB"
                     /locus_tag="ACHK49_001678"
     CDS             5916..6776
                     /gene="tesB"
                     /locus_tag="ACHK49_001678"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO9262344.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(6825..8111)
                     /gene="amtB"
                     /locus_tag="ACHK49_001679"
     CDS             complement(6825..8111)
                     /gene="amtB"
                     /locus_tag="ACHK49_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO9262345.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(8141..8479)
                     /gene="glnK"
                     /locus_tag="ACHK49_001680"
     CDS             complement(8141..8479)
                     /gene="glnK"
                     /locus_tag="ACHK49_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO9262346.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(8660..10441)
                     /gene="mdlB"
                     /locus_tag="ACHK49_001681"
     CDS             complement(8660..10441)
                     /gene="mdlB"
                     /locus_tag="ACHK49_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO9262347.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(10434..12206)
                     /gene="mdlA"
                     /locus_tag="ACHK49_001682"
     CDS             complement(10434..12206)
                     /gene="mdlA"
                     /locus_tag="ACHK49_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO9262348.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHRALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(12236..12694)
                     /gene="decR"
                     /locus_tag="ACHK49_001683"
     CDS             complement(12236..12694)
                     /gene="decR"
                     /locus_tag="ACHK49_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO9262349.1"
                     /translation="MLDKIDHKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(12847..13665)
                     /gene="cof"
                     /locus_tag="ACHK49_001684"
     CDS             complement(12847..13665)
                     /gene="cof"
                     /locus_tag="ACHK49_001684"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO9262350.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGRVTKICFCGGHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            13765..15465
                     /gene="ybaE"
                     /locus_tag="ACHK49_001685"
     CDS             13765..15465
                     /gene="ybaE"
                     /locus_tag="ACHK49_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO9262351.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKHIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGNSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPYWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15530..16225
                     /gene="queC"
                     /locus_tag="ACHK49_001686"
     CDS             15530..16225
                     /gene="queC"
                     /locus_tag="ACHK49_001686"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO9262352.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(16277..16675)
                     /gene="fadM"
                     /locus_tag="ACHK49_001687"
     CDS             complement(16277..16675)
                     /gene="fadM"
                     /locus_tag="ACHK49_001687"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO9262353.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(16769..17140)
                     /gene="ybaV"
                     /locus_tag="ACHK49_001688"
     CDS             complement(16769..17140)
                     /gene="ybaV"
                     /locus_tag="ACHK49_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO9262354.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(17291..19162)
                     /gene="ppiD"
                     /locus_tag="ACHK49_001689"
     CDS             complement(17291..19162)
                     /gene="ppiD"
                     /locus_tag="ACHK49_001689"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO9262355.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKVVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(19354..19644)
                     /gene="hupB"
                     /locus_tag="ACHK49_001690"
     CDS             complement(19354..19644)
                     /gene="hupB"
                     /locus_tag="ACHK49_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO9262356.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(19835..22189)
                     /gene="lon"
                     /locus_tag="ACHK49_001691"
     CDS             complement(19835..22189)
                     /gene="lon"
                     /locus_tag="ACHK49_001691"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO9262357.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(22377..23651)
                     /gene="clpX"
                     /locus_tag="ACHK49_001692"
     CDS             complement(22377..23651)
                     /gene="clpX"
                     /locus_tag="ACHK49_001692"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO9262358.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(23777..24400)
                     /gene="clpP"
                     /locus_tag="ACHK49_001693"
     CDS             complement(23777..24400)
                     /gene="clpP"
                     /locus_tag="ACHK49_001693"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO9262359.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(24647..25945)
                     /gene="tig"
                     /locus_tag="ACHK49_001694"
     CDS             complement(24647..25945)
                     /gene="tig"
                     /locus_tag="ACHK49_001694"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO9262360.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
BASE COUNT         6313 a         7091 c         6252 g         6520 t
ORIGIN      
        1 tttaataatc cataatcatt tagaggctat tcttaattat ttgcggtaat tctttattca
       61 ttcctcggtt attacgtcat attcagagca atcctggtat tagtgtcacc aatttcatct
      121 ggcgataatc ctgaaatgtt atgaacagtt cgagcaaact gcttttacct gctgcgggtt
      181 agtgctaata tgaaaaagtg agtcctgtcc cgattccttc ctaattgtaa tttttcgtaa
      241 taatgcgatg aaaacctgca aagagtggct tatagttaag ctaacaaacg agagggcaag
      301 tccaggtcag taagtttttt ccatcccgaa aggtgtccgt tagttcaacc actaagaagg
      361 ggacgcgtta tggatgaata ctcacccaaa agacatgata tcgcacagct taagtttctc
      421 tgtgaaaccc tgtatcatga ctgtcttgca aaccttgaag aaagcaatca tggctgggtt
      481 aacgacccaa cctcggcgat caacctccag ttgaatgaac tgattgagca tattgcgacc
      541 ttcgcactta attacaaaat taagtataat gaagacaata agctcattga gcagatcgac
      601 gaatatctgg atgacacctt tatgttgttc agcagttatg gtattaatat gcaggatctt
      661 cagaaatggc ggaagtcagg taatcgacta ttccgttgtt ttgtcaatgc gacgaaagag
      721 aatcctgcga gtttatcttg ttagaattat tacaaccatg ggtagaagta tgtccgaaaa
      781 acctttaacg aaaaccgatt atttaatgcg tttacgtcgt tgccagacaa ttgacacgct
      841 ggagcgtgtt atcgagaaaa ataaatacga attatcagat aatgaactgg cggtatttta
      901 ctcagccgca gatcaccgcc tcgccgaatt gaccatgaat aaactatacg acaagatccc
      961 ttcctcagta tggaaattta ttcgctaata aataattcgc tttcggagct ataaccggct
     1021 gtttattaag aattttatac tttttcacca tgaagacata ccctatgtga tctttatcac
     1081 acagatgtaa taggaacgtt ctcttcactg acttttcgtc ttactgtgtt gccgcatttt
     1141 cagcaaccgg aggtcagtaa tgagcacaga aaaagaaaag atgattgctg gtgagttgta
     1201 tcgctcggca gatgagacgt tatctcgcga tcgcctgcgc gctcgtcagc ttattcaccg
     1261 atacaatcat tccctggcgg aagagcacac attacgccag caaattctcg ctgatctatt
     1321 cggtcaggtg acagaggctt atattgagcc aacgtttcgc tgtgactatg gctataacat
     1381 ttttctcggt aataattttt tcgccaactt cgattgcgtg atgcttgatg tctgccctat
     1441 tcgcatcggt gataactgta tgttggcacc aggcgttcat atctacacgg caacacatcc
     1501 catcgaccct gtagcacgta atagcggtgc tgaactgggg aaacccgtca ccatcggtaa
     1561 taacgtctgg attggcggac gcgcggtcat taaccctggt gtgaccattg gtgataacgt
     1621 cgtggtagcc tcaggtgcag ttgtcacaaa agatgtcccg gacaacgttg tcgtgggcgg
     1681 taatccagtc agaataatta aaaaattgta atcggttttt cgcaactgtt atgcaaaatt
     1741 gtggtagatc tgttacttcc cctctactat tcccacgtta aaatagggtg ttccctggaa
     1801 agttgcagat accacgaagg caaacgatga ccgaaataca acgcctgctg accgaaacga
     1861 ttgagtctct gaatacccgc gaaaaacgcg acaacaaacc ccgctttagt atcagtttta
     1921 tccgtaaaca tccggggctg tttatcggta tgtacgttgc tttttttgcc accctggcgg
     1981 tgatgttgca gtccgaaacg ctgtcaggct ctgtctggct actggttgta ttatttatcc
     2041 tgcttaatgg tttcttcttt ttcgatgtct acccacgcta ccgctatgaa gatatcgacg
     2101 tgctggattt ccgcgtttgc tataacggcg aatggtacaa cacgcgcttt gtacctgccg
     2161 cgctggttga agccatcttg aactctccgc gtgtcgcgga tgttcataag gaacaactgc
     2221 aaaaaatgat cgtccgtaaa ggtgaactgt ctttttacga tatttttacc ctcgctcgcg
     2281 ccgaatcaac atcttaagtt agggttacat accaggcgta aagctctgcg cctggtcaaa
     2341 tgacaatgat cgtttccacc catcacttca tgaaatacca gctctacctc cttatctcct
     2401 gccagccttt ttccacaatc agatatactt tccctacact gtgttaataa ggatatgctg
     2461 gtgagaacac gacatctggt cggccttatt tcgggagtac tgattctttc agtattgctg
     2521 cctgtcggct taagcatctg gctggcccat cagcaggtag aaacatcgtt tattgaagag
     2581 ctggatacct attcctcccg cgtcgctatt cgggccaata aggtggcgac acaagggaaa
     2641 gatgcgctgc aagagctgga aagatggcaa ggcgctgcct gtagcgaagc ccatctcatg
     2701 gaaatgcgtc gggtatctta cagttatcgc tatattcagg aagtggttta tatcgataac
     2761 aacgttcccc agtgttcgtc tctggagcat gaaagtccgc ccgatacctt ccccgagcca
     2821 ggtaaaattt cgaaagatgg ttatcgtgtc tggttaacat cgcataacga tttaggcatt
     2881 atccgttaca tggtcgccat gggaacggca cattatgtcg tcatgatcga ccccgcttcc
     2941 tttattgatg tcattcccta tagctcatgg caaattgatg ccgccattat tggcaatgcc
     3001 cataacgttg tcataaccag cagcgatgaa attgctcagg gaattattac caggctacaa
     3061 aaaacacccg gtgagcatat cgaaaataat ggaatcattt acgatatcct gcccttcccg
     3121 gagatgaata tttcgatcat cacctgggct tcaacgaaaa tgttgcagaa aggctggcat
     3181 cggcaagtct ttatttggtt accgctcggg ttggtgattg gccagctggc agcgatgttt
     3241 gtgctgcgta ttttgcgccg tatccagtca ccgcatcatc ggctgcagga tgctatcgaa
     3301 aatcgtgata tttgcgtgca ctatcagccg attgtctcct tagccaatgg caaaattgtc
     3361 ggtgctgagg cactggcgcg ctggccgcag acagacggta gttggttgtc accagatagt
     3421 tttattccgc tggcacagca aacgggcctt tctgagccat tgacgctact gattataaga
     3481 agcgtctttg aagatatggg cgactggctg cgtcagcatc cacagcagca tatttcgatc
     3541 aatcttgaat ccaccgtgct cacctcggaa aaaatcccgc aattgctgcg tgaaatgatc
     3601 aatcactatc aggttaatcc cagacagatc gcgcttgaac tcactgaacg cgagtttgcc
     3661 gatccgaaaa ccagcgcccc gataatttct cgctaccggg aggcgggcca tgaaatttat
     3721 cttgatgatt ttggtacggg atattcaagt ttaagttatt tacaggatct ggatgtcgac
     3781 attctgaaga tcgataaatc tttcgttgat gcgctggaat ataaaaatgt cacgccgcat
     3841 atcatcgaaa tggcaaaaac actgaaactg aaaatggtag cggagggaat cgaaaccagt
     3901 aaacaagaag agtggctacg ccagcatggc gtgcactacg gtcagggctg gctctacagc
     3961 aaggcattac cgaaagaaga tttcttacgc tgggccgagc aacatttgtg aatttttggg
     4021 ctgacggcac gccgcacagc ccgtcacgct acctattcgt cgatgattga ctcgaatccg
     4081 ccatagatca ttcgcttgcc gtcaaacggc atggactcgc cgaactcttt catccgtggg
     4141 tccgacatca tcttttgatt agcagcgtcg cggacctctt ttgaagggta ttcaatccag
     4201 ctaaagacaa cctcttcatt ctcttccgct ttcaccgcca tacgaaaatc ggtcactttg
     4261 ccatccggta catcgctggc ccagcattcg acaatacgaa gcgcgccaaa ctctttaaac
     4321 aatggcgcag ccttagcggc catttcccga taggcatcct ttttatcggc aggaacggca
     4381 accacaaaac catcaacata cttcatcaga catacctccg aagcgtactg cgcagccaga
     4441 caattcttgc gacgtcagtt gtggttaaag tttagccatg ttggtttttt ttgacgaaac
     4501 ggtgggaggc agaaatgggt gggggccctg ccagctacat cccggcacac gcgtcatctg
     4561 ccttggctgc ttccttccgg acctgacctg gtaaacagag tagcgttgcg ggagaaccaa
     4621 caggccccca ttgagagcgt tgagaaccaa cgcgcaggcg cattatcact gctgccccgg
     4681 ctaattgcaa cccatcgcgt gccagatgct ggtttcttgc gcaatgcgac ttcactcggg
     4741 cgtttttcca gattatgctg aaaaattacc tcaggaagaa aagatggaaa aagaagattc
     4801 atttccccaa cgcgtctggc aaatcgtcgc cgctattccc gaaggctatg tcaccactta
     4861 cggtgatgtg gcgaaactgg cgggatcgcc ccgcgccgcg cgccaggtgg gcggtgtgtt
     4921 aaagcgtctc cctgaaggca gcaccttacc ctggcaccgg gtggttaatc gccacggcac
     4981 aatttcgcta accggaccgg atttacagcg tcagcgacag gcattactgg cagaaggtgt
     5041 gatggtatcg ggaagcgggc aaatcgactt gcagcgttat cgctggaact actaaccctc
     5101 tccgggcgga gagggttaac gtcgcttaat attgcgtagg agctggaact gccgaagacg
     5161 gattcacctg agttggtgaa gtcgaaggta cggtagtcgt tgcaccaccg ctggcctgaa
     5221 ccggcacggc ggtttgctgc accggcacca atgtcaggtc ggctttagtt ccgcccttgt
     5281 tgatcaccgg ctgaacggta tcggtaataa ataccagttt gtcattcacg gtaatcgccg
     5341 cactcaacag aatacgcgcg ttcggctgaa catctgccgg attaaatggc agaacaaagc
     5401 tgaatggtga ctgtttacct tcggtacgca ccgctttctg cgccagcact tttgacggtg
     5461 catcggctaa cgacgcgtca gaaagtgtca cggtcagcac agcatcaggc ggcagtgcga
     5521 ctttctgccg gatccagacg gtaccggaga cattcggttg ctggatagca ggttgttgtg
     5581 ttgctgaaat agatgtattt gcagccgggg ctggcgtctg aatatccgcg cttttatctg
     5641 cgcaagccgc caacgcaatc gcaaccgcta aaccactggc catgtgcacg agtttcattc
     5701 atttctcctt attatcaatg caccagcggg ctaactttcc tcgccggaag agtggttaac
     5761 aaaatagtaa cgtcaacaag tgtggcacac atcacgcatt tctgcctgta attagcccgt
     5821 aattcagacc attgcaccca tcggaccagt agcaaatttg cgttatactc aactcacttt
     5881 ggcttgctgc ggcagctttg ttactggaga gttatatgag tcaggcgcta aaaaatttac
     5941 tgacattgtt aaatctggaa aaaattgagg aaggactctt tcgcggccag agtgaagatt
     6001 taggtttacg ccaggtgttt ggcggccagg tcgtgggtca ggccttgtat gctgcaaaag
     6061 agaccgtccc tgaagagcgg ctggtacatt cgtttcacag ctactttctt cgccctggcg
     6121 atagtaagaa gccgattatt tatgatgtcg aaacgctgcg tgacggtaac agcttcagcg
     6181 cccgccgggt tgctgctatt caaaacggca aaccgatttt ttatatgacg gcctctttcc
     6241 aggcaccaga agcgggtttc gaacatcaaa aaacaatgcc gtccgcgcca gcgcctgatg
     6301 gcctgccttc ggaaacgcaa atcgcccaat cgctggcgca cctgctgccg ccagtgctta
     6361 aagataaatt catctgcgat cgtccgctgg aagtccgtcc ggtggagttt cataacccac
     6421 tgaaaggtca cgtcgcagaa ccacatcgtc aggtgtggat ccgcgcaaat ggtagcgtgc
     6481 cggatgacct gcgcgttcat cagtatctgc tcggttacgc ttctgatctt aacttcctgc
     6541 cggtagctct acagccgcac ggcatcggtt ttctcgaacc ggggattcag attgccacca
     6601 ttgaccattc catgtggttc catcgcccgt ttaatttgaa tgaatggctg ctgtatagcg
     6661 tggagagcac ctcggcgtcc agcgcacgcg gctttgtgcg cggtgagttt tatacccagg
     6721 acggcgtgct ggttgcctcg accgttcagg aaggggtgat gcgtaatcac aattaaaaaa
     6781 aatagccgga ggtaaaaccg tccggctatt ttttgcagtg cttgttacgc gttataggca
     6841 ttctcgccgt gactgttgac atccagccct tctcgctcct gctcttccgg tacacgcaga
     6901 ccaaccgtca gatccgccag tttgtagcca ataaatgcca ccacaccgga ccagacgatc
     6961 gtaatggcga tgctttccag ctgtaccagc aactgatggc ccatcgtcac accttcagcg
     7021 aagcccacgc cacccagcga actggcggca aaaatcccag tcatgataca gccgacaatg
     7081 ccacaaacgc cgtgcacacc gaagacatcg cagggatcat ccacccgcag caagcgtttg
     7141 agcatagtaa cgccccacaa gcccgccaga ccagctacca cgccgataat caacgcgccg
     7201 ccaaccccaa tgtagccaca cgctggcgtc acgccgacca gaccggcaat cgcgccagaa
     7261 cacgctccca gcagagaagg cttaccacgc agcgcccatt caccgaagat ccagccaaga
     7321 attgccgccg ccgttgcgac cacagtattc acaaatgcca gtgccgcgat ttcattcgcc
     7381 gtgcccgctg acccggcgtt aaagccaaac caaccaatat agagaatggc agtaccggtg
     7441 aagaccattg gcaggttgtg cggtttaaac gcctctttgc cgaagcccac gcgtttgcct
     7501 atcagatacg cgcccaccag accggcgatt gcggcgttaa tatgcaccac cgtgccaccc
     7561 gcgaaatcca gcgcaccgtg agaagccagc aaaccaccgc cccacaccat atgcgcaatc
     7621 ggaatgtaag agagcgtcag ccataccacc acgaaaatca acacagctga gaagcggatt
     7681 cgttccgcca gcgccccaac gatcaagccg acggtaatgc aggcaaacga gccctgaaac
     7741 gccacgtgga tatactgata aatgctgccc atcaccgccg tcagttcgat gttttttagc
     7801 atcaaccagt taatgttgcc gaagaagttg ttgccctcac caaacgccag cgagtaaccg
     7861 taaaccaccc agagaataca gaccagtgca aacgtcaccg tcacctgcgt cagcatcgac
     7921 agcacgtttt tgccgcgaat caacccaccg taaaacaggg caatccccgg aatagtcata
     7981 aacagcacca gcgcagtaca aatcatcata aacgcattgt cggctttatc ggccaccgca
     8041 ggtgcagcca ttaccagtcc cggcagcatc gccagtgaag caagcccagt ttttatcgtc
     8101 gctatcttca tttttcgttc ctgttgctgt gtgccagaga ttacagcgcc gcttcgtcgg
     8161 cttcgccggt acgaatacga atgacgcgtt gcaattcagc gacgaagatt ttgccgtcgc
     8221 caatttttcc ggtgtaagcc gccttactga cgatatcgat cacttcatcg agttggtcat
     8281 cagcaatcgc cacatcaatt tttacttttg gcaggaagtt gacgctgtat tccgcccccc
     8341 ggtacagctc ggcatgccct ttctgacgcc cgaaaccttt cacttcggtg acggtcaggc
     8401 cctgaatacc aatggaagat aacgcttcac gaacgtcttc cagcttgaat ggtttgatta
     8461 tcacggtcac cagcttcata gatcccctcc ggtcagaatt cggtaatggt tctgctacac
     8521 gaagaaggta ttgcaagcgg tgtgccagaa atgaaaaacg agaaagagag caggaagtta
     8581 agaagattga aaatgaaaac gcactatgac agtgcacagt gcgttacatt tttgcaccaa
     8641 cgatgaaata ttgcgctatt caggcgctca atgattcttc ttcacgcacg ctggctgcca
     8701 gctcttcgcc cgcaagttgc agttgataca tctgccagta gcgtccctgg gccgccagca
     8761 gttgctggtg agtgccctgc tccacggctt gcccacgatg aagcaccaga atggtgtcgg
     8821 catcaacaat ggtcgataag cggtgagcaa tcaccaccag cgtggtatgt tcacgcacag
     8881 ccgccagagc atgttgaatc gcctgttcag taccggagtc aatgctggcg gttgcctcat
     8941 caaggatcag gatttgcggc gtctccacca acacgcgtgc cagtgccagc agttgctttt
     9001 gcccgacgga gagattattc ccctgctcgc ccagcggcgt ataaatacca tcactcatgc
     9061 tgcgcgccag ctccgccagt tgcacggttt ccagcgcctg ccagacgcgt tcttcggaga
     9121 tatcacgccc cagcgtcacg ttggcgagga aggtatccgc cagcaccacc ggatcttgct
     9181 gcaccattgc cacgccctgg cgcagcgcgc tgtgacttag cgaacttaat ggacgaccat
     9241 caaggcgaat ctcaccttcc gttagcgggt aatagcccat caataaactg gcgagggtgc
     9301 ttttgccact gccggtatgc ccgaccagtg ccacaaagtt acgcgaaggc acagagagat
     9361 taatgttctt tagcaccaga ttgtcatcgc gataagcaaa tgacacgtta tcgacttcga
     9421 tggtgccact ctgtaacggg cgatcatcat tgccatattg ctggcgcggt ccgtccatca
     9481 gttcaaacac gcgctcacca gcaacaacag cctgttgcag catcgcctgt tgcgtggtca
     9541 gttcgattaa tggttcgtta agtcgcccaa gatagctgat aaacgcatac agcacgccca
     9601 cttcaatggt gccgctagcg gagaagccaa acagcatcaa caagccacaa agaatgagcg
     9661 atgaaaacag actcagcagc ggacgcagca gaaaaccgtc gaggcgcagg gtttgcatcc
     9721 tggccatata gtgtgaacga ctggcctccc ccatacgttc gccaaatcgc gcctgctgac
     9781 ggaactgctg gataacgctc atgccattga tgatttcgtt aaagccgtcg ttgatatccg
     9841 ctagataggc gcgcacacga cggacaatcg gcgtgctgta acgctggtat atcaccatta
     9901 ccaccagcac caccgggaaa atcattatcg ccaccagcgc cattcgccag tcgaggctga
     9961 acatcgccac cagcatcgcg cccaccagtg cggcgctacg caggacagtt gccactacgg
    10021 taacgtagag atcgcggatc acttcagtgt cattagtgac gcgggaaatc acctgcccga
    10081 cgggttgggt atcaaactcg cttaatggct ggcgcaatgc ggcatccatc acgtcggtac
    10141 gcaactgttg cactacgcct actgccgccc gattaaacag cagcgactgc gcgtaatgta
    10201 gcccggcggc aaacagttgt agcccaacat acgccgcagc cagccctgca accattttca
    10261 acggcaggtt atttttcgct accatattgt cgataaaata gctgataagc agcggcccac
    10321 tgacttctgc cgctgccgca acccacatca tcaggaccgc aatccccagc ggtttacgcc
    10381 acggcgaacc gtacgctaac agacgcttga gagtcggcca cagttggcta aaactacgca
    10441 tcgacggcct cctcgcgaat ttccggagcg tcgtcgagcg ccgcctccag ttgttgatag
    10501 cgatacatat cgcgatacca gccgctttgt tgcgccagct catcatgatt gccacgctgg
    10561 gcgatatgtc cgtgctgcat cacaataatt tcactggctt ccgtcagtgc cgaaaggcga
    10621 tgggcactga tgattaccgt tctaccctgt ccccactgac gcaggttatg caggatctgg
    10681 tgctctgtgc gtccgtccac cgccgaaagc gcatcatcaa ggatgaggat ttccgcgttg
    10741 actaataacg cacgagcaat agagatacgc tgtttttgcc cgccggaaag catcacgccg
    10801 cgctcgccca cctctgtatc gtaaccttgc ggtaaacgca aaatatcgtc atgtacgctg
    10861 gctaaccgcg cgacatgctc aatctcttgc tgggtggcat tcgggcaacc caacgcgatg
    10921 ttgttcgcta cggtgtcgga aaaaaggaac ggtgtctggc taactaccgc caggcggcta
    10981 cgccagctat cgagttgtaa cttcgtcaga ggaatatcat gaaagcgaat atccccctcg
    11041 ctgacgtcga aatgacgctg aatgagcgac aacagggtac ttttgccgga accagtcggc
    11101 ccgcagatac ccagcatctg accgggtttc agggcgaaat tgacgttttc cagcgcacga
    11161 tggtcagtct gcggatacgt gaactggtga atatttacat caagttcgcc acgcccttcc
    11221 ggcacgggtt cgctaccatc gttcaccacc ggcgcttccg ccagcatcgc acgaatacgg
    11281 ctgtacgcag cactaccacg ttccacaatg ttaaacatcc atgccagcgc cagcattggc
    11341 caaatcatca gacctaaata catcataaaa ctggtgagct ggcccagcgt taaactgccc
    11401 tgcaccacca tccaactacc gccgccaatc gccagcaagt tcgccatacc aatcgcgata
    11461 tagattgtcg ggtcgaaacg agcatcaata cgcgccaccc gcatgttttt tttgccggta
    11521 tcttcggcat ccgcggcaaa taacgccgac tggcgatctt ccagaccaaa ggctttgatc
    11581 atgcggatac tggtgaggct ttcctgggtg cggtcattaa gactggaaaa cgccgcctgt
    11641 gccagcttaa agcgttcatg caaggcatcg ccgttgcgct taatcatgat cgccatcact
    11701 ggcatcggta acagggcaaa taaggtcaac tgccagctaa tttgcgtaga catcataatc
    11761 aacacagcgc agcccattac cagtgaatcc accagcgtca gcaccccttc tccggcggca
    11821 aacacgacgc gatcgacgtc attggtcgca cgagccatga gatcacccgt gcgatgacgc
    11881 aggtaaaact ccgggtgctg ccggcttagc tgacggtaat aatcttcacg cagttcaaca
    11941 gccagttggt aagacgcgcc aaacagcaat acgcgccaga cataacgcag gagataaacc
    12001 acaacggcaa tcagcaccat ggtggcgatc cacatcagga tcagcccggt agtaaagtgt
    12061 tgttctgtca cgccatcgac aacaatacca accacttttg gcggaaccag ttgcagcatc
    12121 gcgataatga caagcaaggc gacagccccg agatagcgac gccattcccg acggaaatac
    12181 cagcttaatt gagcaaataa tcgcacgcgt tgtgtcctga cctgattctg gatatttatt
    12241 cgatgggtaa agaagtggtg tatttaatct gttccatcgc gaagctggaa gtgacgtccg
    12301 acagccccgg cacgctgttt accagacgct tataaaactc gtcgtagcgt ttcatgtcgg
    12361 caacctggac gcgcatcaga taatcgtatt caccagccat gcgccagaac cccagcactt
    12421 ctggcatttc ggtaaccacc gtgacaaagc ggcaatacca ttcgctgctg tgatgttgcg
    12481 tttttatcag cacaaaagcg gtcaggccga ggcctatttt ttccggatcc agcagggcga
    12541 ctttgccgat aaggataccg tcgtcctcca gccgtttcag acgcttccag caaggggtgg
    12601 ttgtcagatt aacggcttca gccagtgcct gcaaagagag ggtgcaatcc tgctgcagta
    12661 aggccagcag cttatggtca attttatcta acatagccct tccacagaga atttttttct
    12721 cttttaattc tattttaaag gtgaaatcac gacaatttat tctgtgcggt ttaaacacca
    12781 cggcacaaaa tgacaaattc aacgcaaaaa aaaccgggca attgcccggt gctggaaggg
    12841 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
    12901 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
    12961 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
    13021 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtcag
    13081 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
    13141 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
    13201 aagatcgtcg tgaccgccac agaagcagat cttggtgact ctgccgagtg gcattttttt
    13261 gacatcgatt atctgataac gaaaaccgct atagacaaat gcctgcaaca acgcagggat
    13321 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
    13381 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
    13441 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
    13501 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
    13561 gtcgcgttca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
    13621 catcaataaa gtgccatcca tatcaaatgc tgccagacga gccatttctt tctccactct
    13681 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
    13741 taaataatat tgttccgggt ttatatgcga ttgctcaacc gtcttaacca gtatcaacgt
    13801 ctgtggcaac cttcagccgg aaagccgcaa accgtcaccg tcagcgaact ggccgaacgc
    13861 tgtttttgca gcgaacgcca tgttcgtacg ctgttgcgtc aggcacagga ggcgggatgg
    13921 ctggagtggc aggcgcagtc aggacgcgga aagcgcggac aattacgctt tctggtcacg
    13981 ccggaatcgc tacgcaatgc gatgatggaa caggcactgg aaaccggaaa gcagcaagat
    14041 gtgctggagc tggcgcaact ggccccaggt gagctgcgca ctctgttaca gccgtttatg
    14101 ggcggacaat ggcaaaacga tacacccacg ttgcgtattc cctactatcg cccgctcgaa
    14161 ccgctacaac caggcttttt gcccggccgt gccgagcagc atctcgccgg gcagatattt
    14221 tccggcctga cccgcttcga taataatact cagcgcccga ttggcgattt agcgcatcac
    14281 tgggaaacct ctactgacgg gttacgctgg gacttttatc ttcgttcaac cctacactgg
    14341 cataacggcg atgcagtaaa agcctcacac ttacaccagc gattattgat gctgttacaa
    14401 ctgccagcac tggatcaatt atttattagc gtgaagcata ttgaagtcac ccatccgcag
    14461 tgtctgacct tctttttaca tcgccccgat tactggcttg cgcaccggct ggcgagctat
    14521 tgcagccatc tggcgcatcc gcaattccca ctgatcggca ctggtccttt tcgcttaaca
    14581 caattcacag cggagctggt gcgcctggag agccatgatt attaccattt gcgtcatccg
    14641 ctgcttaaag cggttgagta ctggataact ccgccgcttt tcgaaaaaga tttgggaaac
    14701 agttgtcggc atcccgtgca aatcaccatc ggcaaaccgg aggagctgca acgggtcagc
    14761 caggtcagta gtggcatcag tttaggtttt tgctatttaa cgttgcgcaa aagtccccga
    14821 ctctccctct ggcaggcgcg aaaagtgatc tccattattc atcaatccgg tttattacaa
    14881 acgttagaag tcggagaaaa cctgatcacc gccagtcatg cattactgcc aggctggact
    14941 attccgtact ggcaagtacc ggatgaagtc aaactaccga aaaccttgac gctggtttat
    15001 cacctaccga tagaacttca taccatggca gaacgcctac aggcgacact ggcagcggaa
    15061 ggctgtgaac tcacaattat ttttcataac gcaaaaaact gggacgacac gaccctactg
    15121 gcacacgcag acctcatgat gggcgacaga ttaattggcg aagcaccgga atatactctg
    15181 gagcaatggc tacgttgcga tccactatgg ccacatgttt tcgacgctcc agcatatgca
    15241 catctgcaat cgacgctgga cgcggtgcaa gtaatgcctg atgaggaaaa ccgatttaat
    15301 gccctgaaag cggtttttag ccagttaatg gcagacgcga cgctgacgcc gctgttcaac
    15361 tatcactatc gcattagtgc ccctcccggc gtgaacggtg tgcgactgac accgcgcggc
    15421 tggtttgaat ttaccgaagc ctggcttccc gcgccgtcgc aatgaatagc tggtccgggt
    15481 taatcgcagg cgttaccata acgcttttat tgttcaagca ggattatcta tgaaacgtgc
    15541 tgtcgttgtg ttcagtggag gccaggattc caccacctgt ctggtgcagg cattacaaca
    15601 atatgatgaa gtccattgcg tgacgttcga ttacggtcag cgacatcgcg cagaaatcga
    15661 cgtggcacgc gaactggcgc tgaaactggg agcacgcgcg cataaggttc ttgatgtaac
    15721 gctgctcaac gagctggcgg tcagcagcct gacacgtgac agcattccgg tgcctgatta
    15781 tgaacctgaa gccgatggca tcccgaatac gtttgtccct gggcgtaata ttttgttcct
    15841 gacgctggcg gcaatatatg cgtatcaggt aaaagcagaa gccgtaatta ccggcgtctg
    15901 tgaaacggat ttctccggct acccggattg ccgcgatgag tttgtgaaag cactaaacca
    15961 tgccgtcagt ttgggcatgg cgaaagatat tcgttttgaa acgccgctga tgtggattga
    16021 taaagcggaa acctgggcgc tggcagatta ttacggcaaa ctggatttag tccgtaacga
    16081 aacgttgacc tgctataacg gctttaaagg cgacggttgc ggtcattgtg cggcatgtaa
    16141 tttacgcgcc aacggtttga atcattatct ggccgataaa ccgacggtga tggcagcgat
    16201 gaagcagaaa accgggttga ggtaattatt ccgggtgtcg ccggatgcgg cttgagcatc
    16261 cggcaccaca aaacgtttac ttaaccatct gctccagctt ttcgcgcaat tccccttcca
    16321 gagctaatgc tttctgcgtt ttaagatcaa tacaaacaaa cgtaataagc gcatccgcta
    16381 ccacctgccc ttccggctcc agtgtaatga cctggcttaa gatgccgctt ttaccgttta
    16441 attgctgcaa ctgactggta atagttaaca ggtcgcttaa taccgctggg cgacgatagt
    16501 taatattgat attgaccacg acgaaggcaa tgttatgggc cgtcatccac tgaaaactgt
    16561 cgctattttc caacccatcc cagcgggctt cttcgagaaa ttcaaggtag cgggcgttgt
    16621 tgacgtgctg gtaaacgtcg agatgatatc cacgaacttt gatttgtgtt tgcatagcgc
    16681 aataacctta cgttgttgtt ataagtacag aggtcataac tggtatgacc tcttcagtct
    16741 ggcaaaaaat tgccactatg caaattaatt acagggttaa taccgccaga ttacgttcca
    16801 ccagcgaatt gcccatcccc ggcacctgct ttagatcctc caccgtttta aacggaccgt
    16861 actcttcgcg ataactgaca atcgcctgcg ctttcttcag gccaacgcca ttcatcgcgc
    16921 gggctagctc ttccgcgctg gcattattaa tgctgacccg ggtgccttct tcgtcactgg
    16981 ctttcgccgg tactgctgct ttactttgtg ctgcaggagc ttccgctttg gtttctaccg
    17041 ccgtcggttt cgccacagaa gctgccgcca gcgcgctatg agacattccg gcacaggcca
    17101 gggacagggt aatgagcagt gctttaattc cgtgtttcat actgttttct ccttgtttgt
    17161 taacagtgga atcaccttaa cgggagtgaa aacagtaaac aaatggcaat gttcagaaat
    17221 ggaaaaggcc gcgaaagcga ccttttcttg ttacagctgc aacgcatttt tttgcgaggc
    17281 ggcttcagga ttattgctgt tccagcgcat cgccaatttt gattttcgcc tctttacgca
    17341 ggttactcat cagagcttca aagacgattt gtgcgttgtt ctgggtgata ccctgcacca
    17401 tcgctttttt ctgatcttcc ggcattgaac cttgtttcac ttcatccagc gccagcagaa
    17461 ccacattacc ttgcatatcg gtcgccatac cgtagctcgg tttgtctttc gctggcagtg
    17521 gcagtgcaaa cgccgcctgg ctaatcgggt cacgaccgga acggcttaag attttcggct
    17581 cgccaaattt cagaccggca gcctgcatag cttccgcacc tttgccggct ttcaaatcaa
    17641 ccagcagttt ctcagcatcc actttcgcct gttgttcagc tttgttgtgc tgaaccagtg
    17701 ccttaacttg ttcctgaaca tctgccaacg gtttcaccgc ttccggtttg tgctcgctga
    17761 tgcgcagcac gaatgcgcgg tcgccgtcta cggtgatgat gtcagagttg atgcccggcg
    17821 cgccgttttc acctaccaga ccgccgttaa agatagcgtc ggcaaccggc ttgaagttca
    17881 actcttccgg caggttatct ttgctaaacc aacccgtctg agtggcttta acgccggcag
    17941 cttgctctgc accggccaga gactccgtgt cgttgcttgc cgcatcgctc actttctgct
    18001 gcagcgcata gtacgcatcg agggcttttt cgtgtttcac tttcgccgca atgtcgtcac
    18061 gtacttcgtc taacgatttc actttcgctg gctgaatgtc gtccagacgt acaatcagga
    18121 aaccgaccga agatttgatg acaccagaca gttgcccttt ttctttcaga ccagcatttt
    18181 tcagttcgtc ggggatagtg gcatcttcta accaacccat atcgccgccg ttacgggcag
    18241 agataatatc ggcagatttt tctttggcta atgcagcaaa atcaccgcct ttattcagct
    18301 catcaagtac cactttcgct tcatcttcag ttttggtctg gatgatgctg tagcgggtac
    18361 gctgcggctg ggtgaattga tcctgatgct gatcgtagta gctctggata tccgcatcgc
    18421 taaccggttg ctgcatcgtt gcggcatcca gcttgatgta actcacgcgg aattgttccg
    18481 gcgtcatgaa attgtttttg ttttgttcgt agtagctggc aatctcctgt tcggtcacag
    18541 gctgcttcgc cgccagcgcg ttaacatcga tagtcgcctc acgcaccacg cgttgttgcg
    18601 cgaccagtgc cgccagctcg tcggtttcac ctttcagcat aaaatcggta ccggcaacac
    18661 cgttaatcag ctgttgggta gtgagctggt tacgcagcgc ctgggcgtac tgatcggcgg
    18721 tcatccccat ctggttgagg ataccgttat agcggctgtt atcaaatttg ccgtcaacct
    18781 ggaaggctgg ggtcgcgaaa atcgcctgtt taacctgctc atcactgata cccagtttca
    18841 gctcacgagc gtactgatcc aacagcgcct cgtcgatcag acgattcagc acctgttgac
    18901 gcagggtttt catatagcct tcgttcgctg ccagctcgga gtattgatcg cccagctgtt
    18961 gctgcatgcg attacgctcg ctgttaaagg cgttctcgaa ctgcccacgg ctgatttcct
    19021 ggtcattcac ttttgcggcg tagttattgc ctccgccaat caggtaacca ctcacgccgg
    19081 tcaatatgaa cgacacgata atgataccga aaataatctt gagcacgaga ctgtttgcag
    19141 ccgtgcgtaa gctgtccatc atggtgtaac aacactccgc tgtagatgac tgtgtacctc
    19201 acgcaacgta tcgatgcgcg caaaggccta ttgtgacaag aaacgggggc aattgtcagc
    19261 ccacaagaca cagaaagtca ccgaatacaa ataaaaaagg cacatcagta gatgcgccct
    19321 tgaacttcgt cacatcccca ctggggacaa cgcttagttt accgcgtctt tcagtgcttt
    19381 acctgcacgg aagctcggta ctttagcagc agcgatggtg atctctttac cggtctgcgg
    19441 gttgcggcca gtacgggcag cacgctcttt aacggcaaaa gtaccaaaac ctaccagtgc
    19501 tacatcatcc ccttctttca gagattcagt tacggaagca ataatagcat ctaacgcacg
    19561 gccagccgca gctttagaga tatcagcccc tgcagcaatc ttgtcgatca attgagattt
    19621 attcactctt ctcttcctct ttataattta tatcgcactt gaatccttca aggtacgaac
    19681 gcgcagcagt tatatcaggc cagccatccc cttacaacac cccttaataa gggcaagccc
    19741 gatccgccat ctaacttagc gagacaaaaa aaggctggca agcccgaatt agcctgccag
    19801 ccctgttttt attagtgcat tttgcgcgag gtcactattt tgcagtcaca acctgcatgc
    19861 cagacggttc attttgcagc gccagagtca gaacttcctc aatgcgcttc acaggatgaa
    19921 tgtccagatc ggcaattacg ttgtcaggaa tctcttccag atcgcgttta ttttcgaacg
    19981 gaattagcac tgttttaatc ccgccgcgat gcgctgccag gagtttttct ttcaaaccac
    20041 cgatcggcag tacctgacca cgcagagtga tctcaccggt cattgccaca tcggcacgaa
    20101 ccgggttacc ggtcaggcaa gaaaccagcg cggtgcacat agcaataccg gcactcggac
    20161 catctttcgg cgtcgcacct tccggtacgt ggacgtggat gtcacgtttt tcgtaaaaat
    20221 cagggttgat ccccagtttt tccgcacgcg cacgaaccac cgttaacgcc gcctgaatag
    20281 actcctgcat cacttcgccg agcgaaccgg tataggtcag tttgccttta cccggaacac
    20341 atgcggtttc aatggtcagc aagtcaccgc ccacttccgt ccacgccaga ccggttacct
    20401 gaccgacacg gttttcgtta tcagcgcgac catagtcgaa acgctgaaca ccgaggtagt
    20461 catgcaggtt atcgccgtta atttcgatat gttttaatga cttatcgagc agtaactgct
    20521 taaccgcttt gcggcacagt ttggagattt cacgctccag accacgcacg cccgcctcac
    20581 gggtgtagta acgaataatg ccgataatgg cgctatcgtc gacggtcagc tcaccttttt
    20641 tcagtgcatt acgttcaatc tgcttcggca gcaggtgacg tttggcgatg ttcagttttt
    20701 catcttcggt ataaccggag aggcgaatca cttccatacg atccagcagc ggtgccggaa
    20761 tgttcatgga gttcgacgtc gcgacaaaca tcacgtcgct gagatcgtaa tccacttcca
    20821 ggtagtggtc gctgaacgct acgttctgct ctggatccag cacttcaagc agtgcagagg
    20881 ccggatcgcc tcgcatgtca gaagacattt tgtcgatctc atcgagcagg aacagcgggt
    20941 ttttcacgcc cactttcgcc attttctgga tcagtttacc cggcatagaa ccgatgtaag
    21001 tacggcggtg accacggatt tccgcttcat cacgcacgcc gcccagcgcc atacggacat
    21061 atttacgccc ggtggctttg gcaatggact gaccaagaga ggttttacct acccccggcg
    21121 gccctaccag gcagaggatc ggtcccttga ttttgttgac acggctttga accgcaaggt
    21181 actcaaggat gcgatctttc acgcgctcca gaccataatg gtcggtatca aggatttcct
    21241 gcgcctgacg caggtctttt ttgaccttgc tgcgcgcatt ccacggtacc tgtaccatcc
    21301 agtcgatata accacgcact acggtcgctt ctgccgacat cggagacatc attttcagct
    21361 tctgcaactc tgcttccgct ttctcttttg cctctttcgg cattttcgcc gcgtcgattt
    21421 tgcgcttcag ggcttcgttt tcgtccggcg cgtcgtccat ttcaccgagt tctttctgaa
    21481 tagctttcat ttgctcgttc agatagtact cacgctggga tttctccatc tgctttttaa
    21541 cgcggttgcg aatgcgtttc tcaacctgca gcagatcgat ttccgattcc atcattgcca
    21601 tcagatattc cagacgttcg ttaacgtcgg acatctccag aacggactgt ttgtcagcca
    21661 gtttcagcgg catatgtgca gcaatggtat ccgccagacg cgccggatcg tcgatgctat
    21721 tcagcgacgt cagcacttct ggtgggattt ttttgttcag cttgatgtag ccttcgaact
    21781 ggctgattgc agtacgcacc agcacttcct gttcccgctc atcaatggtc ggcgactcca
    21841 gatactccgc cttcgcagaa aagtgttcgc cattgtcaga gagcgcagaa atacgcgcgc
    21901 gctgtaaccc ctcgaccagc actttgacgg tgccgtcagg cagtttcagc atctgcaata
    21961 tagaggccac ggtcccgacg gtgaaaagat cgtttacacc cggctcatcc gttgaagctt
    22021 ctttctgcgc gaccagcata atttttttat catggtccat cgccgcttcc agacaacgga
    22081 tagatttttc ccgcccgaca aataagggga tgaccatgtg cggataaacc accacatcgc
    22141 gcagcggcaa tacggggatt tcaatgcgtt cagaacgctc aggattcata gagctctctc
    22201 ttagtttaat ttccgccagg taatcagatg acacgactgt gcttcacgcc atctattaac
    22261 atgtacgtca gtatatgggg atgtttcccc cacattcaac gccgagaata gaggaaaaat
    22321 taaaggggag ataaaatccc ccctttttgg ttaactaatt gtatgggaat ggttaattat
    22381 tcaccagatg cctgttgcgc ttccggctta ccataaatca gcaacggttt gctttgacca
    22441 tcaattaccg actcgtcgat aaccactttt tcgacatctt ccatggacgg cagatcgtac
    22501 atggtatcga gcagtgcggc ttctacgatg gaacgcaggc cacgggcacc ggttttacgc
    22561 gccatcgctt tcttagcgat agcatccagc gcctcgtcac ggaattccag atccacgcct
    22621 tccagattaa acagcgcctg atactgcttg gtcagggcgt ttttcggctc tttgaggatc
    22681 tgaatcagag cttcttcgct cagttcattc aacgttgcga caaccggcag acgaccaata
    22741 aactcaggga taagaccaaa cttgatcaga tcttccggtt caacctgcgc cagcagctcg
    22801 ccttcgcttg ctttgtcgga cttcgctttt accgtcgcgc caaaaccaat gccggagccg
    22861 gtttctacac ggtgggaaat cactttatcc agaccggcaa acgcaccgcc acaaataaac
    22921 aggatcttag aggtatcaac ctgcaagaat tcctgctgcg gatgtttacg cccaccttgc
    22981 ggtggaacag cagctaccgt accttcgatc agtttcaaca gtgcctgctg tacgccttca
    23041 ccggaaacgt ctcgggtaat ggacgggttg tctgacttac gagaaatctt gtcgatctca
    23101 tcgatgtaga caatcccgcg ctgcgctttc tgtacgtcgt aatcgcactt ctgcaacagc
    23161 ttctgaatga tgttttcaac gtcttcgccc acataaccgg cttcggtcag cgtggttgcg
    23221 tcggccatgg tgaacgggac gtccagcagg cgcgccagcg tttcagccag cagcgtttta
    23281 ccggaaccgg tcggaccgat cagcagaatg ttacttttgc ccaactcgac accattgctg
    23341 gtatcgccgt tgcgcagacg tttgtaatgg ttgtataccg cgaccgccag cacttttttc
    23401 gcctgttcct ggccgataac gtaatcgtcc aggtggttgc gaatttcatg cggcgtcggt
    23461 agcgcactgc gttcacgatg cggtgcaact tctttaatct cttcgcgaat gatgtcgtta
    23521 cataaatcaa cacattcgtc gcagatatac acggatggac cggcaatcag cttgcgcact
    23581 tcatgctggc ttttgccgca aaaagagcaa tacagcaatt tgcctgagcc atctttgcgt
    23641 ttatctgtca tgggtcaaaa cctcttcttt gttctttgtg ccgcacacga cgacgcaaat
    23701 gccattctca ggcgcaagcc gcttacagcg ttgtgccgcc ctggataagt atagcggcac
    23761 agttgcgcct ctggcatcaa ttacgatggg tcagaatcga atcgaccaga ccgtattcca
    23821 ccgcttcagg ggcggaaagg aagcgatcgc gctcggtatc acgttcaatc tgttctaatg
    23881 attgacccgt atgaagcgcc ataagttcat tcatgcgccc tttaactttc agaatttcac
    23941 gggcatgaat ttcgatatcg gtcgcctggc cctggtagcc gcccaacggt tggtgaatca
    24001 tcacgcgcga attcggcagg caaaaacgtt taccttttgc ccctgcggtc aacaagaaag
    24061 cgcccatcga ggccgcctgg cccatacaga tggtgctgac atcaggcttg ataaactgca
    24121 tggtgtcata gatagacatc ccggcagtga tcaccccgcc tggggagtta atgtacagat
    24181 agatatcttt ttctgggttt tccgcttcca ggaacagcat ctgcgccaca atcaggttag
    24241 ccatgtggtc ttcaacctgg ccagtcagaa aaatgacgcg ttccttaagt agacgagaat
    24301 agatatcaaa agagcgctca ccgcgtgagg tctgttcaat gaccatcggc accagcgcca
    24361 tatggggtgc aaagttatct cgttcgccgc tgtatgacat ttccgtctcc tggataaaat
    24421 tgaaaaaacc tgctgtaccg attgtaacct tatggacgga ttatcagcta gtccctgtgt
    24481 tatgggtacg gcatcaccat atttcaagca taacaatctt ttgttgttac gctaacaccg
    24541 aaagaggctt ttcgcacggt tccatgcaaa attcatgaca aaaaaaaagc ccaccacctg
    24601 gcggtgacgg gcctttgtgc gaatttagcg cgttatgctg cgtaaattac gcctgctggt
    24661 tcatcagctc gttgaaagtg gtttcttttt cagtcacttt cgctttcgcc agtacagctt
    24721 caacagcctg ttcttccaga gcaacattgc gcatgttgtc catcagttct ttgtttttgc
    24781 tgtagaactc gataacttct ttcggatctt cgtacgcaga agccatctct tcgatcaggc
    24841 ctttcacgcg ctcttcgtca gctttcagct cgttggtgcg gataacttcg cccagcagca
    24901 ggccaacaac tacgcggcgt ttagcctgtt cttcgaacag ttcgcgcggc agttccagag
    24961 cttgtttttc gttgccaccg aaacgctgtg cagcctggcg acgcagaacg tcgatttcgc
    25021 tgtcgatcag cgcagccggt acgtcgatgt cgttagcttt taccagacct tcgatcgcct
    25081 gagacttaac gcggttacgg atggcgctct tcagctcgcg ctccatgttt ttacgcactt
    25141 cagcgcgcag accttctacg gaaccatctt caacgccgaa acgtttgatg aattctgcag
    25201 tcagttccgg cagttcacgc tcttcaactt tcttcaggtt gatagcgaat ttcgctgctt
    25261 tacctttcag gttttctgcg tggtattctt ccgggaaggt cacgtcgatg gtgaactctt
    25321 cgccagcttt gtggcctttg ataccgtctt caaagcccgg gatcatacga ccctggccca
    25381 tcgccagtac gaaatcagac gctttaccgc cttcgaactc ttcgccgtct acagaaccgg
    25441 tgaagtcgat ggttacgcgg tcttctgctt caacagcgcc gtctttttct ttccaggtcg
    25501 cctgctgttt acgcagagta tccagcatgc cgtcaacgtc agcgtcggtc acttcaacga
    25561 tcggtttttc aacttcgatc gcttccagac cctgcagttc aacttccgga taaacttcaa
    25621 actctacaga gtaagtgaag tcttcaccca gcttgtattc gcccggaaca taagtcggtg
    25681 cgccagccgg attgattttt tctttaatga tggcgtcaat gaagttacgg ctcatcaggt
    25741 cacccagaac gtcctggcgt acagacgcgc cataacgctg agcaacgata ttcattggca
    25801 ctttgccttt gcggaagccg tcaatacgta cttttttcgc aacgttgacc agctcgcttt
    25861 taacagcggt ctcgatgctg tcagcagcga tagtaatcgt tacacggcgg ccaaggcctt
    25921 gagtggtttc aactgaaact tgcatcttgt tacctcaaaa aatcacagtg ctcggtcaac
    25981 tcttaccccg caagcacagg ttgttggctt cgcggaaccg ggatgttctt ataatcaatc
    26041 acattccctg ttaccagaat catcccgaaa gcattcaaat aggacgcggc attatagcgg
    26101 catcactttt atgagtcgag aacggttatt gcgcgctgtc gcgtattttt tcacaattcc
    26161 cgggcaattt tcgtct
//
LOCUS       DAZGLJ010000045        25594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000045 DAZGLJ010000000
VERSION     DAZGLJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.045"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(163..341)
                     /locus_tag="ACHK49_001695"
                     /pseudo
     CDS             complement(163..341)
                     /locus_tag="ACHK49_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421665.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            571..1308
                     /locus_tag="ACHK49_001696"
     CDS             571..1308
                     /locus_tag="ACHK49_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HFO9262361.1"
                     /translation="MKKVIAVSTLAMAGMFSAQTLADENKTGFYVTGKAGVSVMSLSE
                     QRFVDGEGAWADKYKGSDKSDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGNAESKY
                     RLSYWESAGGAEFDDAQNKLSVNTLMLNAYYDFRNSSAFTPWISAGLGYARVHHKTSY
                     IYTDNSPAGSEVYSASASKYENNLAWSLGAGVKYDVTQDFSLDLSYRYLDAGDSTLTY
                     KDEDGAKYKSSVDVRSNEFMLGATYNF"
     gene            1732..2628
                     /locus_tag="ACHK49_001697"
     CDS             1732..2628
                     /locus_tag="ACHK49_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HFO9262362.1"
                     /translation="MVDSTRNRSAERLIDILVELQNYGVVSRYNLMKKYNITERTAYR
                     DLNMLSPFIEACGDGKYRLISARAGNQSKESLHKSLARLLDTDAIFPERDEGFWQKLE
                     NRATEKHIRVQFHNPEHTIRDDLRKYLDVLEKAICNSNVCQIAYAGKTRIVHPYKLTN
                     QRSIWYLLATEENKLKSFSLAKIKWLDIKKEKFAKSDEIQSLVSESCDPWVSDKTFDV
                     VLFIYSNIAHYFLRRDLLPYQQLLHKHDNGITLSCKAAHKNQIVPLILYWLPNVEIIE
                     PVWLKEAVLTMLGKYLTAENVS"
     gene            2677..3756
                     /locus_tag="ACHK49_001698"
     CDS             2677..3756
                     /locus_tag="ACHK49_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HFO9262363.1"
                     /translation="MALPFIILGVAAGAAAFGGKKAYDGYQKKSEAEDILEKAKERYE
                     EHKEKFEQAEAQTSSKLTTLGNKELEIGKDFSEFDRIAAELLARLEKEGNKDLKLSVP
                     QHNINKIQNLAISATEYLSTVVAAGVSGAAAGFAVYSGVMAFAAASTGTPIAALSGAA
                     AYNATMAAIGGGSLAAGGMGMAGGAMVLGTAVAAPLIAIAGWAYDRHATKALENAHQC
                     ACDVARAVKKMNLAREHFAKVNQYVDEILAALNRMHAVFIKHYFEPLKSMHSVITEQQ
                     DISVSDEVIVLIDHGYSLAAIMTDIITTPLFKPRKQENGEAVIKDNVIEMETDDNGMN
                     VINKDELDDVLASSVVKFDDFSSQS"
     gene            3803..5374
                     /locus_tag="ACHK49_001699"
     CDS             3803..5374
                     /locus_tag="ACHK49_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HFO9262364.1"
                     /translation="MNVEKFDWTEELHQTMVKSLVTSFGLDFLLLNDRKGGDVDTIHN
                     VRNGIYATEAERQRYEGRGEYDSHHYHSHENYIATNREGKRAHQAGTLTDAYTGEVFA
                     SDDKKNLDHIISAKEIHDDPGRILAERDGAELANDASNLAFTHESLNKSKKADSMDAF
                     ICTLQKNREDTLRQIAELESQATLTEKEKKRLISLRKKADADTERMERADKYAREKYE
                     ATINQSYYLSSKFATAVASASLNSGFRMGTRQMLGLILAEIWFELRERIPHIIRQQRN
                     SFSAGKCLSEITSALRACQERVQEKFSSFLIAFKDGVIGGILSGITTTLLNIFLTTER
                     MIVRLIREMWNNLVQAFKVLVFNPEGLSPGQLAKAVTKLIAAGVAVAGGVMINEAMAQ
                     ILIFPFGSELAAFCGALTTGILGLVMNYYLEYSEVMQRVWAFLDKFKDKFQRSLEYFQ
                     AVNAELDRYLVELTALEFSVDAAALNHFSLHLDSVNSEIERGLLLREEVRRRGIALPF
                     ESGNVASVRDWLNKR"
     gene            5777..5974
                     /locus_tag="ACHK49_001700"
     CDS             5777..5974
                     /locus_tag="ACHK49_001700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9262365.1"
                     /translation="MRLLLKGLVGMALAWLVIGFGMELFFLADRVHPALRVIVVLGML
                     FLLIMIAGGMCRYLLSRKKTS"
     gene            6040..7713
                     /locus_tag="ACHK49_001701"
     CDS             6040..7713
                     /locus_tag="ACHK49_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO9262366.1"
                     /translation="MYLPVTACSASGAGQKYRFYSIIMKINLPFIHWPRISNDKRLVL
                     SVVVFTLSNLLVSSGAHLIYRILFFILALLIIKAMKSITLRLIIAFPFTLLTAADISI
                     SLYSWCTFGTTFNDGFAISILQTDPDEVIRMFRMYVVYVIAFIILFLLFFCSAINKTS
                     SLPSEKVTVITFLLLITVTLYSSFQFALKKQYQINEVDPYIVASRFATYTPFFNLNYF
                     ALAAKEHQRLMTIADTIPHYDLVITDNNTDVFVLVIGESARTDNMSIYGYSRPTTPEL
                     QKQKSRLKLFTQAISGAPYTALAVPLALSADSVLHHDIRNYPDNIINMANQAGFDTWW
                     LSAQSAFRQNGTAVASIAMRARNRIYVRGYDELLLPHLAEALNSNPGSRKLIVLHLTG
                     SHEPVCSNWPRDKAVFKPLDTEEVCYDNSIHYTDSLLGQVFAMLETRRASVMYFSDHG
                     LEYDPTKEHAYFHGGIKPSQQAYHVPMFIWYSPTLGEHVDRQTVNSVFSTAYNDYLIN
                     AWMGVTRPAQPKTPEEVITRWQGKSVVFDASHNVFDYKVLRKNFNELQE"
     gene            complement(7857..8900)
                     /locus_tag="ACHK49_001702"
     CDS             complement(7857..8900)
                     /locus_tag="ACHK49_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO9262367.1"
                     /translation="MNDRLRFEVNDNQGRFVFPDTWFGPLLGEFEEVLDAYDTDEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRLIPESFSG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHKQAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNWHPFPLAVRHNFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQKLLRERIDETLSEEIVQKCRNINGEYVWPWILPF
                     SAAGMKHSSIQHQ"
     gene            complement(9334..10524)
                     /locus_tag="ACHK49_001703"
     CDS             complement(9334..10524)
                     /locus_tag="ACHK49_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9262368.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQATDILLQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNAIRGTYNHAQYLDGRREMMQWYADYIDSLSRQ
                     ESQG"
     gene            complement(10701..10776)
                     /locus_tag="ACHK49_001704"
     tRNA            complement(10701..10776)
                     /locus_tag="ACHK49_001704"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10741..10743),aa:Phe,seq:gaa)
     gene            complement(10882..11589)
                     /gene="yqgA"
                     /locus_tag="ACHK49_001705"
     CDS             complement(10882..11589)
                     /gene="yqgA"
                     /locus_tag="ACHK49_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO9262369.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            11742..11825
                     /gene="yqgH"
                     /locus_tag="ACHK49_001706"
     CDS             11742..11825
                     /gene="yqgH"
                     /locus_tag="ACHK49_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFO9262370.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            11987..14122
                     /gene="speC"
                     /locus_tag="ACHK49_001707"
     CDS             11987..14122
                     /gene="speC"
                     /locus_tag="ACHK49_001707"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO9262371.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(14172..15428)
                     /gene="nupG"
                     /locus_tag="ACHK49_001708"
     CDS             complement(14172..15428)
                     /gene="nupG"
                     /locus_tag="ACHK49_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO9262372.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(15630..16709)
                     /gene="mltC"
                     /locus_tag="ACHK49_001709"
     CDS             complement(15630..16709)
                     /gene="mltC"
                     /locus_tag="ACHK49_001709"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO9262373.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(16774..17049)
                     /gene="yggX"
                     /locus_tag="ACHK49_001710"
     CDS             complement(16774..17049)
                     /gene="yggX"
                     /locus_tag="ACHK49_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO9262374.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(17077..18129)
                     /gene="mutY"
                     /locus_tag="ACHK49_001711"
     CDS             complement(17077..18129)
                     /gene="mutY"
                     /locus_tag="ACHK49_001711"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO9262375.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            18290..19009
                     /gene="trmB"
                     /locus_tag="ACHK49_001712"
     CDS             18290..19009
                     /gene="trmB"
                     /locus_tag="ACHK49_001712"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO9262376.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            19009..19335
                     /gene="yggL"
                     /locus_tag="ACHK49_001713"
     CDS             19009..19335
                     /gene="yggL"
                     /locus_tag="ACHK49_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO9262377.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            19519..20238
                     /gene="yggN"
                     /locus_tag="ACHK49_001714"
     CDS             19519..20238
                     /gene="yggN"
                     /locus_tag="ACHK49_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO9262378.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            20414..21460
                     /gene="ansB"
                     /locus_tag="ACHK49_001715"
     CDS             20414..21460
                     /gene="ansB"
                     /locus_tag="ACHK49_001715"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO9262379.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVAAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            21577..22584
                     /gene="yggM"
                     /locus_tag="ACHK49_001716"
     CDS             21577..22584
                     /gene="yggM"
                     /locus_tag="ACHK49_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO9262380.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQAFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(22640..23941)
                     /locus_tag="ACHK49_001717"
     CDS             complement(22640..23941)
                     /locus_tag="ACHK49_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO9262381.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLVYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(23941..24444)
                     /locus_tag="ACHK49_001718"
     CDS             complement(23941..24444)
                     /locus_tag="ACHK49_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO9262382.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            complement(24489..25475)
                     /locus_tag="ACHK49_001719"
     CDS             complement(24489..25475)
                     /locus_tag="ACHK49_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFO9262383.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         6891 a         5748 c         6323 g         6632 t
ORIGIN      
        1 ttcaacacgc caataaaaaa tctaattcta ttaatcttga cctggtctat caaaatgata
       61 gaccaggctt ctgttatcta cattacttct agtgaaatag ccaaccaatc gtccctataa
      121 ggcaggttaa atagttttaa tttagcaaat ttgataaaca actcactcgc cattaccccg
      181 attgatcttc gcatagtcaa acggcgtaat cgctctctcc cgattggcat ccagaaaatc
      241 tgcccaccat tgcagcatca gcttgcgctc atccagatgc tccgctttat ggatatacgc
      301 tgcctgcact gagttacgtc catgtgggtc atctgacgtt cagccgcttt atctcgtgct
      361 gttctggtct caatacttcc atgccgttgc aattacctct taaaccattc cttatcattt
      421 tcatgtcttc gataagaaat cttcatcatt catgatcgat tttgtcgatc gattcgatcg
      481 aattttctct ttttaccttg tcttttgcat ggttttaaaa tgagcaacct tctttatcgg
      541 gtggggtaag aaccaacgga gatgattgaa atgaaaaagg ttattgctgt ttcaacactt
      601 gcaatggcag gcatgttttc ggcccagact ctggctgatg agaacaaaac tggcttttat
      661 gtgaccggta aagccggtgt ttctgttatg tcactttcag agcaacgttt cgttgatggt
      721 gaaggggcat gggcggataa atacaaaggc agtgacaaaa gtgatacggt ttttggcgca
      781 ggccttgcgg tgggatacga tttttatcag cactacaatg tgccggtgcg tactgaagtg
      841 gagttttatg gccgcggtaa cgctgaatca aaatatcgtc tgagttattg ggaaagtgcc
      901 ggtggtgccg agttcgatga tgcacagaac aagctcagtg taaacacgct gatgctgaat
      961 gcgtattatg atttcagaaa cagcagtgca ttcacgccat ggatatctgc tggtctgggt
     1021 tatgcacggg ttcatcataa aacttcgtac atatataccg ataacagccc ggcaggcagt
     1081 gaagtttatt cagcttcagc gtcaaaatac gaaaacaacc tggcatggag cctgggtgcc
     1141 ggtgttaaat acgatgtgac gcaggatttc agccttgacc tcagctaccg gtatctggat
     1201 gcaggggatt ccactctgac ttataaagat gaggatggtg ctaaatataa atcctctgtt
     1261 gatgtcagaa gtaacgagtt tatgttaggc gcaacgtata acttctgaca gccatttcta
     1321 cctgcgcttt ggggcacagt aaagtgcagg tatgacaaat tgtcaacaac agatatcccg
     1381 atgatattct ccctctggca actaataatt ttagatgaat gaaatataaa tagagtaaaa
     1441 atattactcg cgttgatttt ttacagaggt gcaggtgata cgcagtatgg ttgtcagtct
     1501 gttaaatgaa aaaggactgt tgttgggaca cgttgttttt tgtcatatca gcccttccgg
     1561 aaacaataaa ccgtattacg aaggaggctc ccggacatct ggtttaaagt ttgaacataa
     1621 tcaaataaaa atctgttgcc ggagggcgta gttttgtgga actgacaaat gatgtcagga
     1681 gtatttatat aattcactta cacaagagta atactacagg tgcagtgatt tatggttgac
     1741 tcaacgagaa atcgctcagc ggagcgtttg attgatatcc ttgttgaatt acaaaattat
     1801 ggcgtggtca gtcgctataa tttgatgaaa aaatacaaca tcactgagcg aacagcatat
     1861 cgtgatctga atatgctttc tccctttatt gaagcctgtg gcgatggaaa ataccggctt
     1921 atttcagcaa gagcaggaaa ccagagcaaa gaatcactgc acaaatctct ggccaggtta
     1981 ctggatactg atgcgatttt tcctgaacgg gatgaaggtt tctggcagaa attagaaaac
     2041 agggcaaccg aaaagcatat tcgtgttcag tttcataacc ctgagcacac tatcagggat
     2101 gatttaagaa aatatcttga tgttcttgaa aaagcgatct gcaacagtaa tgtttgccag
     2161 attgcatatg ccggtaagac cagaatcgtt catccctata aattaactaa tcagagaagt
     2221 atatggtatt tgctggctac tgaagagaac aagttaaagt cgttttcatt agctaaaata
     2281 aaatggctgg atataaaaaa agaaaagttt gcaaagagtg atgaaataca gtcactagta
     2341 agtgagtcat gtgacccgtg ggtgagcgac aaaacatttg atgttgtgtt gttcatttac
     2401 agtaatatcg cccattattt tttgcgacgg gacttacttc cttaccagca attgctacac
     2461 aaacatgaca atggcattac gctgtcatgc aaggctgcac ataaaaatca aattgtcccg
     2521 ttgattttat actggctacc gaatgttgaa attattgaac cagtatggct gaaagaagcc
     2581 gtgttaacaa tgttaggaaa ataccttact gctgagaatg tttcctgaaa tatttcagtt
     2641 gactgatgat aaagcaatct gtacaggagg aaaagaatgg cgttaccttt tattattttg
     2701 ggtgtggctg ccggcgctgc ggcattcggc ggaaaaaaag cttacgatgg atatcaaaaa
     2761 aagtcagaag cagaggatat cctcgaaaaa gcaaaagagc gttacgagga gcataaagaa
     2821 aaatttgagc aggctgaagc tcagacatca tcaaaattaa caacgctcgg aaataaagag
     2881 cttgagattg gtaaagattt cagtgagttc gacaggattg ctgctgagct cctggcgcgt
     2941 ctggaaaaag agggcaataa agacctgaaa ctgagtgtcc cgcaacataa catcaataag
     3001 attcagaacc tggccatttc agcgacagaa tatctcagta cggtcgttgc tgccggagtt
     3061 tccggggctg ctgcgggttt tgctgtgtat agcggagtga tggcatttgc tgcggcttct
     3121 acaggtacac ccattgcagc gctttctggt gccgcggctt ataacgcgac gatggcggca
     3181 attggagggg gctctctcgc ggcagggggc atgggaatgg ccggcggcgc aatggtatta
     3241 ggcaccgcag tggctgcacc gttgattgcc attgcgggct gggcttacga cagacatgcg
     3301 acgaaagctc ttgaaaatgc ccatcagtgt gcctgcgatg ttgcacgggc agttaagaaa
     3361 atgaatctgg cccgggagca ttttgcaaaa gttaatcaat acgttgatga aattctcgct
     3421 gcactgaaca gaatgcatgc tgtatttatt aaacactatt ttgagccgct taaatccatg
     3481 cattctgtta ttacagaaca acaggatatc agtgtatcag atgaagtcat tgtgcttatt
     3541 gaccatggat attcactggc cgctattatg acggatatta ttacaacgcc actgtttaaa
     3601 ccgcggaaac aggaaaatgg tgaggcagtg ataaaagata acgttattga aatggaaacg
     3661 gatgataatg gaatgaacgt tattaataag gatgagctgg atgatgtact ggccagttcg
     3721 gttgtgaaat ttgatgattt ttcatcccag agttaatgat ttaccgggcg ttaaagcccg
     3781 gctttacaag aagtgaaata acatgaatgt ggaaaaattt gactggacag aagagttaca
     3841 tcagaccatg gtgaagagcc tggttacatc attcgggctg gattttctgt tgctaaatga
     3901 tcgtaagggt ggtgatgttg acactatcca taatgtgcgt aatggcattt atgccacgga
     3961 ggctgagcga cagcggtatg aaggtcgtgg cgaatatgat tctcatcatt accacagcca
     4021 tgagaactat atcgcgacga accgggaagg aaagcgtgcg catcaggcag ggactcttac
     4081 tgatgcatat acaggcgagg tgtttgcttc tgacgacaaa aaaaatctgg atcatattat
     4141 ttccgctaaa gaaattcatg atgatccggg gagaattctt gctgaacggg atggtgcaga
     4201 actggcaaat gacgcttcaa atctggcatt cactcatgag tcgctcaata aatcaaagaa
     4261 agcagactca atggatgcgt ttatttgcac gctgcagaaa aacagagaag atacgttaag
     4321 gcagatagca gagcttgaat ctcaggcaac gctgacagaa aaggaaaaga aacgtctgat
     4381 aagtctgaga aaaaaagctg atgcagatac tgagcgtatg gaaagggctg ataaatatgc
     4441 ccgggaaaaa tatgaggcca cgattaatca gagctattac ctgagtagta agtttgctac
     4501 tgctgtcgcc agtgcttcgc ttaattccgg ttttcgtatg gggacgcgac agatgttggg
     4561 gcttattctt gccgagatct ggttcgaact gagggaacga atcccgcata ttatcaggca
     4621 gcaacggaac tcattcagcg ccggcaaatg cctgtccgaa ataacgtcag cattacgtgc
     4681 ctgccaggaa cgagtacagg aaaaattcag ttctttttta attgccttta aagatggtgt
     4741 catcggaggg atactgtcag gtattaccac aacgctgtta aatatattcc tgacaacgga
     4801 aagaatgatt gtccggctta tcagggaaat gtggaacaac ctcgttcagg cctttaaagt
     4861 cctggtattt aatccggagg gattatcgcc ggggcagtta gccaaagcgg tcactaaact
     4921 gatagcagca ggtgtggctg ttgccggagg ggtgatgata aatgaggcga tggcgcagat
     4981 ccttattttc ccatttggtt cagagcttgc tgccttttgt ggtgcgctga cgacaggtat
     5041 tctcgggctg gtgatgaatt attatctcga atatagcgag gttatgcagc gggtatgggc
     5101 atttctggac aaatttaagg acaaattcca gcggtcactg gaatactttc aggcagtcaa
     5161 tgctgaactt gatcgctatc ttgtggaact tactgcactg gaattttctg ttgatgctgc
     5221 cgcgttaaat catttttcac ttcatctgga tagcgtaaac agtgagatag agcgcggact
     5281 tctgctgaga gaagaagtcc gccgacgagg gattgcgttg ccgtttgagt caggaaatgt
     5341 ggcttctgtt cgtgactggt taaataagcg atgaaattac accctccatt tccctgtaac
     5401 cagtgtgggg cctgttgtcg tcatgtcaac agggcgggtg aaacgcaatg tctggatcgt
     5461 ggtgacggta tttgccgtca ctatcagact gattctcatt tgtgtgcaat ttatgataaa
     5521 cgcccacaga tttgccgggt tgaagaccag tatttactga attatcagtc tcagtacagc
     5581 tggcaggagt ttattgcatt aaaccaggcc gcatgcctta ttctgaataa actgtaatgc
     5641 cattaatggc ggcgtcagga cagaaactga gagaacataa aacagtaaaa tgtcatacca
     5701 ttcagcagcc agcgttactt tccccggctg ctgttattgt aaggcatttt cgttaagaat
     5761 acagaaggag aggagcgtga gattgctgtt aaaagggctc gtcggaatgg cgctggcctg
     5821 gcttgttatt ggtttcggga tggagttatt ttttctcgcc gatcgggttc atccggcatt
     5881 aagagtaatt gttgtcctgg ggatgttatt cctgctgata atgattgcgg gtggtatgtg
     5941 ccgttatctt ctgtcacgaa agaaaacatc atgacgttcc tgtttacgcc gttacatttg
     6001 tggcggcgga tgatgcccgt tttatgatta ttcagaaaaa tgtatttacc tgtcacggca
     6061 tgttcagcga gcggcgccgg acagaaatat cgcttttact caattattat gaaaattaat
     6121 cttccattca tacactggcc acggattagc aatgataagc gcctcgtgtt atcagtggtt
     6181 gtctttaccc tttctaatct ccttgtatcc tcaggagcgc atctgattta ccggatatta
     6241 ttctttatcc tggcacttct tattattaag gctatgaaat cgattacgtt gcgtttgatt
     6301 attgccttcc cgttcacatt actgactgct gcagatatca gtatcagcct gtattcatgg
     6361 tgtacatttg gcacaacatt taatgatggt tttgcaatca gtatccttca gacagatcct
     6421 gatgaagtaa taagaatgtt caggatgtat gttgtttatg ttatcgcatt tattatcctc
     6481 tttttattgt tcttctgttc agcaataaat aaaacgtcct cattgccatc ggaaaaggtc
     6541 acggtaatta catttttatt gttaattaca gtaacgttat attcttcctt ccagtttgcg
     6601 ttgaagaaac agtatcaaat aaatgaagtt gatccttaca tagtcgcttc ccggtttgcc
     6661 acatataccc ctttctttaa tctgaactat tttgcactgg ccgcaaagga gcatcagaga
     6721 ttaatgacga ttgctgatac catacctcac tacgatctgg tgattacgga taacaacact
     6781 gacgtatttg ttctggtgat tggtgaatct gcaaggactg ataacatgtc catttacggg
     6841 tattcgcgcc ctaccacacc agagcttcag aaacagaaat cacgactgaa actgttcact
     6901 caggcaatca gtggcgcacc ttacaccgcg ctggctgttc cactggcgtt atctgccgat
     6961 agtgtactgc atcatgatat ccgtaattac cctgataaca ttatcaacat ggcgaatcag
     7021 gcggggttcg atacctggtg gctcagtgcg cagtccgctt tccgacagaa cggaacggct
     7081 gtggccagta tcgccatgcg ggccagaaac agaatttatg tcagaggcta tgatgagtta
     7141 ctgctcccac acctggctga agcactgaac agtaatccgg ggagcaggaa actgattgtt
     7201 ctgcatctga cgggcagcca tgaaccggtt tgcagtaact ggcccagaga taaagccgtg
     7261 tttaaaccgc tggatacaga agaagtctgt tacgataatt ccattcatta taccgacagt
     7321 ttgttgggac aggttttcgc catgctggaa acgcgtcggg catccgtcat gtatttttca
     7381 gatcacggtc tggagtatga cccgacaaaa gaacatgcct attttcatgg tggaataaaa
     7441 cccagtcagc aggcttatca tgttccgatg tttatctggt acagcccgac actgggagag
     7501 catgtcgatc gccagacggt gaacagcgtt ttttccactg cgtataatga ttatctgatt
     7561 aatgcatgga tgggcgtaac cagaccggct caaccgaaaa cgccggagga ggtgattacc
     7621 cgctggcagg ggaaatccgt ggtttttgat gccagtcata acgtgtttga ctacaaagta
     7681 ttacgaaaga actttaatga actccaggaa taattcgtta aagtaattta cgactctatt
     7741 cttttttaaa ggaaactttc agaaaatgcc cccagggcga taacctgggg aagggggact
     7801 gtgttattac tggtggataa attattactt gtttgaaaga tacactaatg caatatctac
     7861 tgatgctgga tgcttgagtg tttcattcct gctgctgaaa acggcaatat ccagggccag
     7921 acatattcac cgttaatatt tctgcatttc tgtacaattt cctcagaaag tgtctcatca
     7981 attctttccc gcaacagctt ctgctgtctt aaaatgcttt cgcatatttc gaaatcatct
     8041 tcctgcatca gcatttcggc gcatttaatg atttctgctc gttcatgcag cacagaggaa
     8101 tggttataga gccagtttac aaacagcaat gcttccggat attgtcccca taatggtgag
     8161 tatgttgcat agtaatcttc tgccgtatcc agagagccgg agaagttatg ccgcacagca
     8221 agtggaaatg gatgccaatt tccgcacagc atttccgcga tgtaagtgtt tgtagcaaag
     8281 ccatgacgaa aagctgtggc agccttaaca tgttccccat tcagaaaatg cagaagacca
     8341 agttcatacc agtaaggtga aaattcatca gcatgctctt tcagtacact gaaggcttgc
     8401 ttatgatcgc ctgttcgtaa cagttctgag cccaacaacc agcgggcacc ctgattatct
     8461 tcaggattgt atgccagtat tttatcagta agcataaccg catcctgatg acgctgtaag
     8521 tgcacatttg cgagaattgc tgcataaagc gcgcgcagat aaggccggtt ttcgggatgc
     8581 atccagatga tttcaccact gaaactttcc gggatgagac gattacctgc cgccagtcct
     8641 ttaagcgcgg cgttcagggc cttacgcggg gcattctgct ccagaaatgc atatgccaga
     8701 tgagcgtgta tatcaataaa gtcaggctcc cgttgagcaa ggcgtctgag tttgttgatg
     8761 taactcgtct cgctgatttc atcagtgtcg tatgcatcga gcacttcttc aaactccccc
     8821 agtaatgggc caaaccaggt atccggaaat acgaaacgcc cctggttatc gttcacttca
     8881 aagcgtaatc tgtcgttcat tttctgagcc tgtaaaacgg atgggacaca atctgaaaag
     8941 cataaaagtc gtgatgttgt gtatcaagtc ttgtttttgt ccgggatgaa tgttgtttac
     9001 aatatcatcg gcagtggttt tacatgagaa tgaaaattta tctttatctg cattccggtg
     9061 tattgtgaat tgtcggctga aacggcatat taatcgattt ttttagggga atggtgctga
     9121 aataacgaca tgatgatgat ttatattaca ggtatcaaaa ttgttagcgg tccataacgg
     9181 tgtgatgttt tgagattgtt tacagatgca atattaaata ttttgtcaaa aaacaaaaaa
     9241 tatctaaaaa taaagcatga aaacaaaatt cctccggacc tggaggccgg aggagaactt
     9301 gtggattgag agaaaatatc agaacaagtg ttcttaaccc tgactctctt gcctggaaag
     9361 cgaatcaatg taatctgcgt accattgcat catttcacgg cgaccatcga gatactgggc
     9421 atgattatag gtacccctga tggcgttttt atccacatga gctaactgca tttcaatcca
     9481 ggcagaatta aagccctgtt cgtgcagaat ggtactcatg gtgtgacgaa aaccatgacc
     9541 ggttgccctg ccatcgtaac cgatacgttt gagcaccata ttgatgctgg cttcgctcat
     9601 tggcttgttg atatcacaac gtcccgggaa aaccagccgg tattgtccgg agaaagtttt
     9661 gagctgcagc agaatgtcag tggcctgtcg tgacagtggt acgacatgtt tacgtcgcat
     9721 tttcatgcgg ctttcaggga ttgtccacag ggcattatca aaatcaattt ctgaccattc
     9781 agcggaccgt agttcaatcg ttctgaggcc tgtcagaagc agcagatttg tcgccatgcg
     9841 ggtaacagga ctgccatgat attcagcgag cttccggaga aaaacaggga gctcatctgc
     9901 tctcaggtgg gggaaatgtg cagcttttgg cgccgccagc gtactggtca gttcagatgc
     9961 cggattgaca gtggctcgtc cggtggcgat ggcataacga aaaatctgat tacagtattg
    10021 acgcacctta cgaagttttt ccagcgagcc acgtttttct atgcggcgca gaatttccag
    10081 catttccagc gggaggatat cagtaacgct tcgtttgccc agatacggga aaatatcttt
    10141 atccagcgct tccagtacgc ttctggcgta actttcagac catgacaaag atttatgctg
    10201 gtgccattcc gtagcgaggg catggaacgt attggtggca gagattttct gagtgagttt
    10261 ttcctttcgc ttcttttcgc ccgggtcacc accttcggag ataatttttc gggcatcgtc
    10321 gcgtttgatt ctggcttcag caagggagac gtcgggatag acgccgaggg ccagtttttt
    10381 ctcttttcct gctatccggt atttaagccg ccagtagcgg gcacccgtgg tttttaccag
    10441 cagatataaa ccacgttcgt cagagagttt atagtctttt tctttaggtc tggctgtttc
    10501 gacctgtctg gctgtaagtg ccatatgggg gctcctgaaa ctgaccgaac acaatgagcc
    10561 cccaccggtg cccccaatga acgcttgatt gagagagagt tcagtagagt tcggtggagt
    10621 tcaggatgtt cagattgctt gtaacgatgt gattatactt gagtttcgtg gagttcggga
    10681 gatgaatctg gaagtgaaag tggtgcccgg actcggaatc gaaccaagga cacggggatt
    10741 ttcaatcccc tgctctaccg actgagctat ccgggcaacg gggcgcatta aacctgattc
    10801 gcctcgtctc gtcaatcaaa tttcttcaat ttactgcaga ctgcacaatc tatcatcact
    10861 ttgccgctat tgcacgcatt gtcaggcaaa ccaggcggtc caggcggcgg aaagcggcat
    10921 tgccagaagg agtgctggaa gcatgttgac caccgggaac attttaatgc cacagatgcg
    10981 taatccggtt gccagcagca ataaaccgcc tacggcgctg aagtctgcca tcatcgacgg
    11041 tgtggtcagc ggtaatatca gcgcggcagc ccacgccagc gttaactgga tgatcagtaa
    11101 tgggatacta attaccgaca ccgcaatacc cagcgagcag gcgaagatca tcgccgtaaa
    11161 gaaatcaaga aatgacttgg cgattaaaat actcggatcg ccggtcatcc cttcgttcat
    11221 cgccccgaag atcccggtgc cgctggcgca aaacaggaca ataatcgcga cataattctg
    11281 aataaaagat tcatgcgctg gcttcttacg tgagtgacga aacagatttt gtgctttggc
    11341 gactgctgta ttgacacctt tctccagcaa acaaatttcg ccaattagag cgccaagtag
    11401 cgtagctaaa accatcgctg gaaggttggc gcatttcaca accagtaaaa taccaatccc
    11461 cagcgatgcc agaccaaaaa tagatgtcat ggagacgcgg atacgttccg gtaagcgttg
    11521 gctgagcagt gcgccgagaa cgccacccag taagactgca ctagcgttga taaaagggcc
    11581 gatgaccaca agagttcctg ttagctgtat tgtttgattt tcttattatg aactttttgc
    11641 cttgttgatg gcttgtctgg tcgcaaagtc ctggcttgca cgctttagcg aaaggtgcca
    11701 tgattgcgcg aattttctcc tctctgtacg gagtttgccc gatgcacgcc atctccttac
    11761 attctctcgc ttatcgccgt ttcgcgcgaa acgtttccct ttctatgtta ctgctcatgc
    11821 ggtgaagtta acgcacgctc actgcaggac aacagtaaaa tcagagcgtt tctgctttta
    11881 ctgatgtctg gcggtcggag ctggtgacca gtttgaccca tatctcatgg ggcagggttt
    11941 tccaccttgt ccggtattct tacttccccg aaacgggttt gcgcttatga aatcaatgaa
    12001 tattgccgcc agtagtgaac tggtatcccg actttcttct catcgtcgcg tggtggcgtt
    12061 gggagatact gattttacgg acgtcgcggc agtcgtcatt accgctgcgg atagtcgcag
    12121 tggcattctt gcgttgctta agcgcaccgg ttttcatcta ccggtgtttt tgtattccga
    12181 acatgctgtt gaattacctg cgggcgttac ggcggtaatc aacggcaacg agcagcagtg
    12241 gctggagctg gaatccgcag cctgtcagta tgaagagaat ttgctgccac cgttttatga
    12301 cacgctgacg cagtacgttg agatgggcaa cagcaccttt gcttgccctg gacatcaaca
    12361 tggtgcgttt tttaaaaagc atcctgccgg acgccatttt tacgatttct ttggtgagaa
    12421 cgtctttcgc gccgatatgt gtaacgctga cgtaaaattg ggcgatctgc ttattcatga
    12481 aggatcggcg aaagatgcgc agaaattcgc agccaaagtc tttcatgccg ataaaaccta
    12541 ttttgtgctg aacggcacat cggcagcgaa taaagtggtg acgaatgcgc tgttaacgcg
    12601 tggcgatctg gtgctcttcg accgtaacaa ccataagtcg aatcatcacg gcgcgctgat
    12661 tcaggcgggg gcgacgccgg tctatctgga agcttcacgc aacccgtttg gtttcattgg
    12721 cggtattgat gcgcactgtt ttaatgaaga gtatctgcgc cagcaaattc gcgacgttgc
    12781 gccagaaaaa gccgagctgc cgcgcccgtt tcgcctggcg attattcagc tgggaaccta
    12841 tgacggaact gtctataacg cccgtcaggt gatcgatacc gttgggcatc tgtgtgatta
    12901 cattctgttt gattccgcgt gggtcggtta cgaacaattt atcccgatga tggcggatag
    12961 ctcgccgctg ctgttagaac ttaacgaaaa cgatccgggg atttttgtga cccagtcggt
    13021 gcacaaacag caggctggat tctcacagac gtcgcagatc cataaaaaag ataaccatat
    13081 ccgcggacag gcgcgttttt gcccgcataa gcggttgaat aacgccttta tgctccatgc
    13141 ttctaccagc cctttctatc cgctgtttgc tgcactggat gttaacgcca aaattcatga
    13201 aggggagagt gggcgtcggc tgtgggctga gtgcgttgag ttgggtattg agtcgcgcaa
    13261 ggctattctt gcgcgctgta agctgttccg cccgtttatc ccgcccgttg ttgatggcaa
    13321 attgtggcag gattatccga catctgtgtt agccagcgac cgccgttttt tcagttttga
    13381 gccgggggcg aagtggcacg gctttgaagg atatgccgcg gatcagtatt ttgttgatcc
    13441 gtgcaagctg ttactcacta caccaggtat caatgccgaa actggcgaat atagcgactt
    13501 tggcgttccg gcgacgattc tggcgcacta tctgcgtgag aacggcattg tgccggagaa
    13561 gtgcgatctc aactccattc tgttcttatt aactccggcg gaaagccacg agaagctggc
    13621 acaactggtg gcgatgctgg cgcaatttga acagcatatt gaggatgact cgccgctggc
    13681 tgaggtgttg ccgagcgttt ataacaaata tccggtgcgc tatcgcgact acaccctgcg
    13741 ccagttgtgt caggagatgc acgatctgta tgtcagtttc gacgtcaaag acctacaaaa
    13801 agcgatgttc cgccagcaga gtttcccgtc agtggtgatg aacccccagg atgcgcatag
    13861 cgcttatatt cgcggtgaag tggagttggt gcggattcgt gatgccgaag ggcgaattgc
    13921 ggcagaaggg gcgttgcctt atccacctgg cgtgctttgc gtggtacccg gggaagtctg
    13981 gggtggggcg gttcaacgtt atttccttgc actggaagaa ggggtgaatt tgttgccggg
    14041 attttcgccg gagctgcaag gtgtttatag cgaaaccgat gcggatggca tgaaacggtt
    14101 gtacggttat gtgttgaagt aagaataaaa aaaacgggtc accttctggc gacccgtttt
    14161 tctttgcgta attagtggct aaccgtctgt gtgcctgtcg ggacacgaac gtgtttatat
    14221 ttgaacatcg ccatgaacgc gaaggccaga accacggagt aaccagcgaa aatcaaccat
    14281 acggtctgcc agtcggtaat gccgttttgg gtgtacatct caacaacttt accgctcacg
    14341 atgccgccga ggatacagcc gaagccgtta gtcatcatca ggaacatccc ttgtgcactg
    14401 gcgcgaattg ccgggctaac ttctttttcg acaaacaccg aaccagagat gttgaagaag
    14461 tcgaatgcgc aaccgtaaac gatcatcgac agtaccagca gtacagtacc gaacggagtc
    14521 gggtcgccgt aagcaaacag cgcaaaacgc aggatccacg ccacaatact gatcatcatt
    14581 acgttcttaa taccgtagcg gcttaagaag aacgggatgg tcagaatgaa cagggtttca
    14641 gagatctgcg aaatcgacat gatgattgac gcatgctgca caataaagct gctggcaaac
    14701 atcggatctt tgtcgaagct gtgcaggaag gtattaccga acatattggt aatctgcagt
    14761 tccgcgccca gcagcattga gaagataaag aagattgcca tacgcttgtt tttaaacagc
    14821 gcgaatgcat cgaggcccag cagggttgtc cagctctgat tcgcttgctg tttagcaacc
    14881 ggaatatgcg gcagagtcag ggtaaacaga accagaatgg cggaaagtgc tgcgccaata
    14941 tacagctgca tgtggcttaa ttcgaagcca gacaggctca ccacccacat tgccatgata
    15001 aagccgatgg tgccccagat acggattggc gggaagtcag taacgatatc catcccggca
    15061 ttttgcaggc gatagtaaga gatggtgttg attaacccaa gcgttggcat ataagcaaac
    15121 gagttaatca atatcacaag gaacatcgcc tccggtgtcg tgacctgtgc cgccatgaac
    15181 agcgtgatag cgccaatggt gtggcaaatg gcatataccc atttcgcact taaccatttg
    15241 tcggccacaa tccccagcag cgcaggcata aagaccgcgg cgatacccag tgagctataa
    15301 actgcgccaa tagaagcacc gtcaaacttc agggtaacaa acatatagga gccgagggtc
    15361 gtcagccaac ttccccacag acagaactgc agaaaagaga ggattttcag ctgcagctta
    15421 agattcatgt taatttcctc acatcgtgat gcggatgagt gtttgaaaaa gcacatttga
    15481 ccgtgcgaga tgatgggatt ctatcaactt cgcggactgg ttttttgtta cctgtggcaa
    15541 ataattgcaa acattcccaa tttgcaaaac gaaattgtga tgtggataac atttttgccc
    15601 ctgagcatcg tcaggggcgg ttaatggaat tatcggcggc ggtaggattt ttgcgcggta
    15661 ttcactttat aaagataacg gcgagattcc gcagagggat ggcgggtcgt cagcgtctga
    15721 taaacatcgc ccggcgtcat ggtgttaata atattggcag cctgaatctt atcattcgaa
    15781 aagactcgca gcacgctgcc tgcgccgccg ttataggcgg tgatgacggc ataacgccgc
    15841 gatgttgggt tatcaattcc gccgagataa acattgttca gcatcgccag atacgcggtg
    15901 ccggtatcaa tattgctggc aggatcaaac aagaaactgc ggctcggcgt gccggatttc
    15961 ccctgcgagc ggaacacatc tttcccggca gtatgttgta ccacctgcat taatcccagc
    16021 gcatcggaac ggctgaccgc atatgggtta aaggaagatt cggtctgcat aatcgccaga
    16081 atcagcgact catcaacgcc atatttccgt gacgcctggc ggaccatgcc gagatatttg
    16141 tgcgcacgtt tatcaaggtg gttcggcacc atgttaatgg taacgctgta gatgatacgc
    16201 agcccgttgc tgcggctctt cagacggttt ttcagcagat aatccgcgaa gttgctcgcg
    16261 cgaccttccc agcgaatcgg ctgcccggtg ttgtccacca cctgaccgta aaggaaaggt
    16321 tctttcgaaa tcgtaatatc atcaacgtcg gaatagagat cgaccgaact cggatcgtca
    16381 cccatcagca acgttttgat aattgcccgg cgcaaatgcg cggcaggttc tgtcccggcg
    16441 atagtttcga tagtaatcgt accgtcatcg aagttgatgt ggctgcgggt ctgatattga
    16501 tcggtgtatt tcacgtagtc cttaggaccg gcgatcacca cctctttgaa tccccagatg
    16561 ttctcaatat tgtgggcaaa ttgccccatc agaatatcaa aaccgttggt atctttgacc
    16621 caggcttcgt tataggtatc gccttttttg gtcgtcgaac aggagatgag caacggcgca
    16681 atcaaagcca gcgcgagata ttttttcatc attccgggtg cgtgttgtgt ttgtttttat
    16741 gcaagttgcc ggaggcgtgc tccggcactg tttttatttt ttatcttccg gcgtatagcc
    16801 ctcgatatgc acctctttac cctcgaacag gaagttgacc atctcctgct ccagcagctt
    16861 gcggtgctcg gcattcatca tgttgagttt cttttcatta atcagcatgg tttgtttgtg
    16921 ctgccactgc gcccaggctt ctttggagat ctcgttatag atgcgttttc ccagctcgcc
    16981 ggggtacagc tgaaaatcct gaccttctgc ttcacgttgc aggaaagtac aaaaaatcgt
    17041 tctgctcata aatcatcctc tttatcgact cacgcgctaa accggcgcgc cagtgcgtaa
    17101 ctgctgtaac aaacgctcca cgggagccgc caggccaact gacggcggtt gcgctaagtt
    17161 ataccagagc gcattgcctt catccatgca gccggtgaat gacgacacgg gaagccacat
    17221 aggcacaata tctaagtgga aatggctgaa ggtatgccga aacgcggtca gctgcgtcag
    17281 gttatcggca gcaatctgcc gttgcgccag ccactgccgc aaactttctt cgtcggcaaa
    17341 ctgcgggaaa cagtataaac cgccccacaa tccgctcggc ggacgctgcg ccagcaatac
    17401 ttcatcttcg tgctgtaata gcaaaaagta gccggtgcgc tccggcagcg tctgtttcgg
    17461 ttttttgccc ggataaagcg cccagctatt gttggcggcg gcaatacatc cgttttgtag
    17521 cggacagagc gaacatttcg gtttcgagcg cgtacaaatc atcgcaccca aatccatcat
    17581 cgcctgatta aaccgttcca cgccaaccgc gggcgtcacc tgctcgctca aactccataa
    17641 tttattctcg acctcttttt tccctggcca gccgcttaca gcatagcagc gcgccagcac
    17701 ccgtttgacg ttaccgtcga gaatcggaaa gtgcttaccc agagaaagcg agagaatcgc
    17761 acctgcggtg gaacgcccga cgcccggtaa cgccgcgact tcttcaaagg tttccgggaa
    17821 tttaccgccg tgtaaggtcg ccacttgttg tgccgcttta tgcaggttgc gcgcgcgggc
    17881 gtaatagcca agcccggtcc acaagtggag aacttcgtcg agcggcgcat tggcgagatc
    17941 ggtcaccgtc gggaagcgcg ccataaagcg ttcaaaatag gggataacgg tcgcaacctg
    18001 agtttgttgc aacatcactt ctgagagcca tactttgtag ggcgtcttgt caatttgcca
    18061 gggcagcgtt ttccgcccgt atttatcgta ccagtccaga acctgggctg aaaattgcga
    18121 cgcttgcatg gtcaccgaat tcactgttgt tgggggcaag attgcagcac agcgacggca
    18181 gggtgtaaac cggaactttc cgcagccacg gccttatcat gcttgcatcc ggcaactaac
    18241 tttggataat gcccgttttc agaacacttt cacaagcgac taaatcttta tgaaaaacga
    18301 cgtcatttca ccggaatttg atgaaaacgg ccgcccactg cgccgtatcc gtagttttgt
    18361 gcgccgccag gggcgactga ccaaaggcca ggaacatgcg ctggaaaact actggccggt
    18421 gatgggcgtt gagttcagcg aagatatgct ggatttcccc gcgctttttg gccgtgaagc
    18481 gccggtgacg cttgagattg gttttggcat gggggcgtcg ctggtggcaa tggctaaaga
    18541 tcgccctgag caggacttcc tcggcattga agtgcattca ccgggcgttg gtgcgtgcct
    18601 ggcttctgcg catgaagaag gtttaagcaa cctgcgcgtg atgtgtcacg atgcggttga
    18661 agtgctgcat aaaatgattc ctgacaattc attgcgcatg gtgcagctct ttttccctga
    18721 cccgtggcac aaagcgcgcc ataataaacg ccgtatcgta caggtgccgt ttgccgaact
    18781 ggtaaaaagc aaactgcagc tggggggcgt attccatatg gcgaccgact gggaacctta
    18841 tgcggaacat atgcttgaag tgatgtcttc tattgacggt tataaaaacc tgtcagagag
    18901 caatgattac gtaccgcgtc cggcatcacg tccggtgacg aaatttgaac aacgtggtca
    18961 tcgtcttggt cacggagtat gggacttaat gttcgagagg gtgaaataat ggcaaagaac
    19021 cgtagccgtc gtctgcgtaa aaaaatgcac atcgacgaat tccaggaatt aggattttcg
    19081 gtggcatggc gattcccgga aggtacatcg gaagaacaga ttgataaaac cgttgatgat
    19141 tttattaacg aggttatcga accgaacaaa ctggcctttg acggcagcgg ttatctggcc
    19201 tgggaaggtc tgatctgcat gcaggaaatc ggcaaatgca ccgaagaaca tcaggcgatt
    19261 gtgcgtaagt ggctggaaga gcgcaaactg gatgaggtac gcaccagcga acttttcgac
    19321 gtttggtggg actaagaaag catacgggcg atgacaaatg caaaactgcc tgatgcgcta
    19381 cgcttatcag gcctggaaag atgcacgatc gagtaggcgg gataaggtgt ttacgccgca
    19441 tccggcatgg aaaacgcgta ctttgttatc aatctggggc cagcaaatgc tggcctgatt
    19501 ttttcttgag ggaagactat gatgcgcaaa atgctgctgg cggcagcact ttcagtgacg
    19561 gcaatgaccg ctcacgccga ctaccagtgc agcgtcacgc cgcgtgacga tgtgattgtc
    19621 agcccgcaaa ccgtgcaggt gaagggcgaa aacggcaatc tggtgatcac gccagacggc
    19681 aacgtgatgt ataacggtaa gcaatattcc ctgaatgccg cccagcgcga gcaggcgaag
    19741 gattatcagg ctgaactacg cagcacgctg ccgtggattg atgaaggcgc gaaaagccgc
    19801 gtcgaaaaag cccgtattgc gctggataaa attatcgttc aggagatggg cgaaagcagc
    19861 aaaatgcgca gccgtctgac caaacttgat gcgcagctga aagagcagat gaaccgcatt
    19921 atcgaaacgc gcagcgatgg cctgacgttt cactataaag ccattgatca ggttcgcgcc
    19981 gaaggccagc aattagtgaa tcaggcaatg ggtggaattt tacaggacag cattaatgaa
    20041 atgggcgcga aagcggtgct gaaaagcggc ggtaacccat tacagaatgt gctgggaagc
    20101 ctgggggggc tgcaatcctc aatccaaacc gagtggaaaa agcaggaaaa agatttccag
    20161 cagtttggca aagatgtttg tagccgcgtt gtgactctgg aagatagccg caaagccctg
    20221 gtcgggaatt taaaataatc ctctatttta agacggcata atactttttt atgccgttta
    20281 attcttcgtt ttgttacctg cctctaactt tgtagatctc caaaatatat tcacgttgta
    20341 aattgtttaa cgtcaaattt cccatacaga gctaagggat aatgcgtagc gttcacgtaa
    20401 ctggaggaat gaaatggagt ttttcaaaaa gacggcactt gccgcactgg ttatgggttt
    20461 tagtggtgca gcattggcat tacccaatat caccatttta gcaaccggcg ggaccattgc
    20521 cggtggtggt gactccgcaa ccaaatctaa ctacacagcg ggtaaagttg gcgtagaaaa
    20581 tctggttaat gcggtgccgc aactaaaaga cattgcgaac gttaaaggcg agcaggtagt
    20641 gaatatcggc tcccaggaca tgaacgataa tgtctggctg acactggcga aaaaaattaa
    20701 caccgactgc gataaaaccg acggcttcgt cattacccac ggtaccgaca cgatggaaga
    20761 aaccgcttac ttcctcgacc tgacggtgaa atgcgacaaa ccggtggtga tggtcggcgc
    20821 aatgcgcccg tccacgtcca tgagcgcaga cggtccattc aacctgtata acgcggtagt
    20881 ggccgcagct gataaagcct ccgctaatcg tggcgtgctg gtggtgatga acgacaccgt
    20941 actggacggt cgcgatgtca ccaaaaccaa caccaccgac gtagcgacct tcaagtctgt
    21001 taactacggt cctctgggat acattcacaa cggtaagatt gactaccaac gtaccccggc
    21061 acgtaagcac accagcgata cgccattcga tgtctctaag ctgaatgagc tgccgaaagt
    21121 cggcatcgtt tataactacg ctaacgcatc cgatcttccg gctaaagcac tggtagatgc
    21181 gggctatgat ggcatcgtta gcgctggtgt gggtaatggt aacctgtata aatccgtgtt
    21241 cgacaccctg gcaaccgccg cgaaaaacgg cactgcagta gtgcgttctt cccgcgtacc
    21301 gacgggtgct accactcagg atgctgaagt ggatgatgcg aaatacggct tcgtcgcctc
    21361 tggcacgctg aacccgcaaa aagcgcgcgt cctgctgcag ctggctctga cgcaaaccaa
    21421 agatccgcag cagatccagc agatcttcaa tcagtactaa tcacctcgcc ccggtatcgt
    21481 gccggggctt tttcacttca gacttacgtc cattgccaat tttcattacc ctaatgataa
    21541 tcaccggaat aaattattcc gcgtgaggtt tttcgggtga aaaagcaatg gattgttggc
    21601 acggcactgc ttatgttgat gactggtaat gcctgggcag atggcgaacc gccaactgaa
    21661 aatatcttaa aagatcaatt caaaaagcag tatcacggca ttctcaagct tgatgctatc
    21721 accttaaaaa atcttgatgc taagggtaat caggccacct ggtcagcgga aggcgatgtc
    21781 tcttccagtg acgatctcta tacctgggtc ggtcagttgg cagattacga actgctcgaa
    21841 cagacctgga cgaaagataa accggtaaaa ttctcggcga tgttaaccag taaaggaacg
    21901 cccgcgtctg gctggtcggt gaacttttac tcttttcagg cggcagccag cgatcgtggg
    21961 cgggtggtcg acgatatcaa aacgaataat aaatatctga tcgtgaatag cgaagatttc
    22021 aattatcgct ttagtcagct tgaatctgcg ttgaataccc agaagaactc gattcctgcg
    22081 ctggaaaagg aagtgaaagc gcttgataag caaatggtgg cggcgcagaa agcagcggat
    22141 gcatattggg gaaaagatgc aaacggtaag caaatgaccc gcgaagaagc ctttaaaaaa
    22201 attcaccaac agcgtgatga gtttaataaa cagaacgata gcgaggcgtt cgcggttaaa
    22261 tatgacaaag aggtttatca accggcgatt gcggcatgtc ataaacagag tgaagagtgt
    22321 tatgaagtgc cgattcagca gaaacgagat ttcgatatca acgaacaacg gcgacaggct
    22381 tttctgcaat cacaaaaact cagccgtaaa ttgcaggatg actgggtaac gcttgaaaaa
    22441 gggcaatatc cgttaacgat gaaagtttca gaaataaaca gtaaaaaagt cgcgattttg
    22501 atgaaaattg acgatatcaa ccaagctaat gaacgctgga aaaaagatac cgaacaactg
    22561 cggcgcaatg gggtgattaa gtaagtttga tatagtgcct ttgccggata atataaaaag
    22621 ttatccggca aattattttt tacattagcc ctagtagctg tggaatgaac aaggatattt
    22681 gcggaatgta agtgatcatc atcaacgcta ctagcaatgc agcataaaat ggcagcaatg
    22741 gtttgattaa atgttgaatc ttcacacccg agatagaaca accaacaaat agcgcactac
    22801 ccaccggcgg tgttaacagc ccaatacaaa ggttggccac catcataata ccgaaatgta
    22861 ctgggtccat tcccagctct tgtgcaatcg gcaagaagat cggtgtaaag attagtacag
    22921 ccggtgtcat gtccataaag atgccgacaa ttaataagac gatattaatc agcagcaata
    22981 tgatcagtgg gttatcagaa atccccatca atgcgtcgct gatcatataa ggaatatcag
    23041 cattagtcat tgcccaggac atgcccacgg aaaaaccgat caacagtaat acaatcgaag
    23101 tggtaacaac cgactccaga atcagtttgg gtaaatggcg ccattttact tcccggtaaa
    23161 ccagcaccga gagaataaat gtgtagacaa cggcaattgc tgacgcttca gttgcggtaa
    23221 aaatgccgcc cagaatgccg cccatcacaa tgaacaccag aagcaaactt ggtaaggcat
    23281 ccagaaacgc tttacaggcc tgagcaaagg ttgctctttc tgaaagcggg tagccacggc
    23341 gtttagcaat aatgccgcag actatcatta ccgcaagacc cattaagatc cctggcaaat
    23401 atcccgccat aaatagcgat gctacagaaa cgccgccagc tgtcagggaa aacacaatta
    23461 acacgtttga gggcggtatc agtaatccag taatacaaga tgagacgttt acagcggtag
    23521 aaaatgccgg atcataacct tgtttggtct gaatgggatt cagtgtgcca ccaacggcag
    23581 cagctgccgc aacggctgaa ccagaaatgg aaccaaacat catattcgcc aggacgttca
    23641 catgaccaag tgaaccagga acccgaccaa ccattacctg ggcgaggtta atcaaacgta
    23701 tcgcaatacc gccgctgttc atcagcgttc cggcaaagat gaaaaagggg attgccaata
    23761 gggcaaagtt atccagtcca ttagccagtc gctgcgaaac agtgattgcc gcgatatccc
    23821 agggaaacat caaaagcatt gaagctacag tggcaatacc gattgagaac gaaatgggca
    23881 caccaataaa gaccaacaca aaaaaacagc caaacagtgt aatcgcgata taactatcca
    23941 ttaactggcc tcctgcgcat gttgtttcag ttgttgaatg ctttgattaa taaaacacaa
    24001 ggcataaaac atcatcacca atccacttaa tggcagaatg atatatacgt atcccatcgg
    24061 tatttgcatc gcggcagata cctgtgatgt tgccagcgtt ttatcaatta atttcaggcc
    24121 accagtcaca atgaccgaac cggcaaaacc taaaataaga acgttaatga ctatactaag
    24181 aagtaattgc ttgcgcttat tcagggcgag agcaaataaa tcgatagaga gatgtctctg
    24241 cgcgccgacg gtatatgcag cacctaataa ccctacccag atcatcaaaa atcgtgccag
    24301 ctcatcggtt aatgtacttg gctggttaag tacgtagcgg ctaaatactt gccagactac
    24361 gcatacaacc agagcaagca tgacggcgac agaaaacgcg gcaatgacgc ggtctaccgc
    24421 aagtttcagg cggttaaata tcattgtaag catcctggaa aatcagggcc agagactggc
    24481 ccggaatatt attgagcagc gttgtcgaac ttctctaaca aagcagcttg ttttggatct
    24541 tttttgaagt cgtcaaacag aggctgaact gcggcacgga atggtgcttt atccactttt
    24601 acaatttcac cccccatagc tttcgcctga gcacgtgtgt cagcgtcaat tttttcccac
    24661 aatttttgtt gataagcttc ggatttcttc gctgctgttt cgaggatttg ttgctgttct
    24721 ggggttaatt tattccatgt tttggtggag ataaccaaaa agtcaggaat tgaggcgtgt
    24781 tcatcttcag aaaaaacttt ggcaatttca atatgtcgag tctgcaccca ggaaggcacg
    24841 ttattttctg cgccatctac tacaccttgc tgcatcgcag tataaacttc gccaaatgaa
    24901 attggagtgg gtgaaccacc catcagttca atcattttga tggtggtcgg acttggctga
    24961 acacgaattt tcagcccttt aagatcttct ggcttagtaa tcggtttttt cgcatagaaa
    25021 ctgcgtgtac ccgctacata agctgataaa gcaaaaaagc ctttgtcttt tgttgagtcc
    25081 ataatttctt taccaacttc accaaaaaca actttgttga agtgagcctg atctttaaat
    25141 aagaacggca aattatagat agcataaata ttatcaaaag actccagatc actggcggaa
    25201 cctttggtca tatccagagc gccattttgc aacaactcca tagtttctcg cgcattaccc
    25261 atctggctgc tgggataaat acggatcacc atattacctt ttgacagctg ttttacctct
    25321 ttcgccagct cttcaaaact ctgatgtaca acatgacttc tttccaggtt gtgtgctaac
    25381 tttaacgtca ctttttctgc ggcgtttacg ccagtgctaa ccagagccag caaggatgcg
    25441 cagactaatg agcgggtaaa tgacctcatt ttcataaaaa aactccctgt tgaaggatgg
    25501 aaattggttg atatctaatt gtgcttatca gtgctttgtg cgacaaattg gttaacctgt
    25561 ctgacaactt gtctatgttt atcacaactt gaaa
//
LOCUS       DAZGLJ010000046        25392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000046 DAZGLJ010000000
VERSION     DAZGLJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.046"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            139..669
                     /locus_tag="ACHK49_001720"
     CDS             139..669
                     /locus_tag="ACHK49_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9262384.1"
                     /translation="MSQGKEPVIERIGMLTKRYPSRSAAARAWGININTLNSYFKGGQ
                     TQPAPRESILIRISESEGVSLEWLKEGKGESPNDVGICGNFGCSGDKGDQLTQMLSFL
                     TKDEREQLTNLLARKGVETVLTLLDKENQDLLQLEGRKRLAALMIVDMPDDCVREILE
                     KTETSRQSNPVESKAG"
     gene            complement(921..1415)
                     /locus_tag="ACHK49_001721"
                     /pseudo
     CDS             complement(921..1415)
                     /locus_tag="ACHK49_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
     gene            complement(1494..2231)
                     /locus_tag="ACHK49_001722"
     CDS             complement(1494..2231)
                     /locus_tag="ACHK49_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HFO9262385.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGAGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLEKNAEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(2231..3433)
                     /gene="flgE"
                     /locus_tag="ACHK49_001723"
     CDS             complement(2231..3433)
                     /gene="flgE"
                     /locus_tag="ACHK49_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO9262386.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVMCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFNASD
                     EAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPVDLTVDYSTCTQYGSEFSVTTN
                     AANGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(3457..4170)
                     /locus_tag="ACHK49_001724"
     CDS             complement(3457..4170)
                     /locus_tag="ACHK49_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HFO9262387.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQV
                     DFSVNPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(4170..4601)
                     /gene="flgC"
                     /locus_tag="ACHK49_001725"
     CDS             complement(4170..4601)
                     /gene="flgC"
                     /locus_tag="ACHK49_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO9262388.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(4604..4939)
                     /locus_tag="ACHK49_001726"
     CDS             complement(4604..4939)
                     /locus_tag="ACHK49_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HFO9262389.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            5021..5758
                     /gene="flgA"
                     /locus_tag="ACHK49_001727"
     CDS             5021..5758
                     /gene="flgA"
                     /locus_tag="ACHK49_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO9262390.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRQWHDYRYTFKIYIPSQIATAAPCTKTPGVTLTSPAEIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMTKSLVARDMPLTANDVELKPYNVSAQRRDVLMELDDAI
                     GLSSKHALQPGRPITKEELVSPVLVGRDQPVMIVYQSAGITASMPGVALKNGRKGEMV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            5839..6117
                     /gene="flgM"
                     /locus_tag="ACHK49_001728"
     CDS             5839..6117
                     /gene="flgM"
                     /locus_tag="ACHK49_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO9262391.1"
                     /translation="MKITPTMPGNRPTATTAGSSQPRKTTVTTTTTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSNKVAQVQAMLASGSPQVDTEQLASDMLSFFQN"
     gene            6130..6558
                     /gene="flgN"
                     /locus_tag="ACHK49_001729"
     CDS             6130..6558
                     /gene="flgN"
                     /locus_tag="ACHK49_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HFO9262392.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRICMIRRASEEL
                     LAVNDEITHHYEQLHGHSHQRHSLLKMLGVSVNRDGLAQVFTWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQSFLGSEADFLYQE"
     gene            6685..7614
                     /locus_tag="ACHK49_001730"
     CDS             6685..7614
                     /locus_tag="ACHK49_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HFO9262393.1"
                     /translation="MSFIECYEPEYVRKFLARHPGSPLYVRKVWKNEIRLSLSLTDIA
                     SCEAVLNDVRAFDLQLTYRPVEDNSAELSARDAIVNQVILSTLTLRDLTPELSLYAVG
                     ILLSCASKMPGRDGDILARLTTLPQALADHAKKGTLQAQYAQLPPVPQLARQLMTLLG
                     GCAFDWSILPESPRKASLPLQVTLLTLHDANSEALLQQQLQTQWQTTWQQHFATAPWM
                     MRNWLIYRVYHDMIGQTDGADYFPLVCDFYLLRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRASENALLVRQQIQSLCAADPLLSAFSLLT"
     gene            7793..10237
                     /locus_tag="ACHK49_001731"
     CDS             7793..10237
                     /locus_tag="ACHK49_001731"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO9262394.1"
                     /translation="MPTPTIIKTLATHREKPFVSVVTPTWNRGAFLPYLLYMYRYQDY
                     PADRRELIILDDSPQSHQHIIDRLTNGTPEAFNIRYIHHPEKLPLGKKRNMLNELARG
                     EYILCMDDDDYYPADKISYTIAMMQKHRALISGSDQIPIWYSHINRIFQSRSFGPHNI
                     LNGTFCYHRNYLKKHRYDDDCNLGEEKSFTDNFSVNPLQLPGERTILCISHSHNTFDK
                     DFIIGASTPVNTALTDIVRDPLLRNAYLSLHNATHHQAINHQAIDQVVVVNLDKRPDR
                     LQQIREELALLHIPPEKITRLAASEDENGQRGRRQSHLQALRLAQQHGWQNYLLLEDD
                     AVILKQEKHIQVLNTLLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAY
                     LVNSRYYPQLAQQMSNDEHSLEDCWQPLLRADKWLACYPSLCYQRPGFSDIQKKTTDN
                     IARYFNKLPVTTKPSALPIADTIGFFMETSFHYALYRPIITALQAQGLSCTLVINDRV
                     FKPFLDEMLETLKNIDDPQLKGMRLSEMQTHGQRVKCLVSPYHTPALNGLAAVNIRAM
                     YGLAKETWNHADWNRFYQSILCYSHYSQQALAHFGSAKVVGNPRFDAWHNGTFDRALP
                     ENIQSDYRKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLICKLHHGTCSRPEEAASL
                     ALVRRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMNDLLD
                     GEKSYSTAESADQRIREILPVAHDVAELRYLLSEAFDWGSVQARLTEIRHHYCDAFMD
                     GKAGERAAIVIVEALG"
     gene            complement(10355..10750)
                     /locus_tag="ACHK49_001732"
     CDS             complement(10355..10750)
                     /locus_tag="ACHK49_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HFO9262395.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARALGERLLVGVSSDALNIAK
                     KGRAPVYHQDDRMAIIAGLACVDGVFLEESLEQKAEYLRGYSADILVMGDDWAGKFDS
                     FAYICEVVYFPRTPSVSTTGIIEVIRGLL"
     gene            complement(10753..11187)
                     /gene="fliJ"
                     /locus_tag="ACHK49_001733"
     CDS             complement(10753..11187)
                     /gene="fliJ"
                     /locus_tag="ACHK49_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO9262396.1"
                     /translation="MRQIIDTLTQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YLVEKTSAGAAVSVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            complement(11190..12527)
                     /gene="fliI"
                     /locus_tag="ACHK49_001734"
     CDS             complement(11190..12527)
                     /gene="fliI"
                     /locus_tag="ACHK49_001734"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO9262397.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESAGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTSLEHQQSARALKQNYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPGVKKAEQ"
     gene            complement(12520..13230)
                     /gene="fliH"
                     /locus_tag="ACHK49_001735"
     CDS             complement(12520..13230)
                     /gene="fliH"
                     /locus_tag="ACHK49_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO9262398.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTTSISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGRKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPA
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALKETLTPESQGE"
     gene            complement(13233..14243)
                     /locus_tag="ACHK49_001736"
     CDS             complement(13233..14243)
                     /locus_tag="ACHK49_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO9262399.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVGRLS
                     ENMARLSGVKTSMARKVINNFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEEVLEQLQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAEQT
                     AE"
     gene            complement(14221..15867)
                     /gene="fliF"
                     /locus_tag="ACHK49_001737"
     CDS             complement(14221..15867)
                     /gene="fliF"
                     /locus_tag="ACHK49_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO9262400.1"
                     /translation="MNAQIKKLTLAFPAFRLRLADNKRWALMAGVGLAVAATAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEEALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLHASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTHASVANVLDSLVGSGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPVAANQMTNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTAQ
                     LTALLNNAAGIDAQRGDNLTLSLLNFVPQAVPVEPIIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPVMKRLTAVRAPIITPESEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            complement(15872..16213)
                     /locus_tag="ACHK49_001738"
     CDS             complement(15872..16213)
                     /locus_tag="ACHK49_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO9262401.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPAMTFSSTDPDVSF
                     HRIMQGALGHVDQFQQVAEQQQTAIDMGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            complement(16228..17214)
                     /locus_tag="ACHK49_001739"
     CDS             complement(16228..17214)
                     /locus_tag="ACHK49_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFO9262402.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENENAPYIGVNCAAIPENMLEATLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQEVAAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYSTVLVKNITLSESTRRAMLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPENDIADHSELQWPAQPAVHIAETSDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKHGIEVFS"
     gene            17601..18452
                     /locus_tag="ACHK49_001740"
     CDS             17601..18452
                     /locus_tag="ACHK49_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO9262403.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKAHNGLPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESCFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSLPLTLN
                     VKIAELSMNVADLTQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENSHE"
     gene            18445..18816
                     /locus_tag="ACHK49_001741"
     CDS             18445..18816
                     /locus_tag="ACHK49_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO9262404.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            18813..19565
                     /gene="fliP"
                     /locus_tag="ACHK49_001742"
     CDS             18813..19565
                     /gene="fliP"
                     /locus_tag="ACHK49_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO9262405.1"
                     /translation="MKRRTQLTLGLGLLALAPVALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTML
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GHAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            19568..19840
                     /locus_tag="ACHK49_001743"
     CDS             19568..19840
                     /locus_tag="ACHK49_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HFO9262406.1"
                     /translation="MLTIDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIIQLHDLCIHLFTQAALLVH"
     gene            19842..20624
                     /gene="fliR"
                     /locus_tag="ACHK49_001744"
     CDS             19842..20624
                     /gene="fliR"
                     /locus_tag="ACHK49_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO9262407.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYVPVLDNSALTVRVRI
                     ILSLALAIIITPLIPHPVPHDLISLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            20614..21753
                     /gene="flhB"
                     /locus_tag="ACHK49_001745"
     CDS             20614..21753
                     /gene="flhB"
                     /locus_tag="ACHK49_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HFO9262408.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPEVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIERLFSSEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFASFLGLQALPFKLAMNEGLSLYACVMRNFVILFIFFALIDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDSSRAAAPFVVAKGTDEIALYIRQVAAENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            21737..23830
                     /gene="flhA"
                     /locus_tag="ACHK49_001746"
     CDS             21737..23830
                     /gene="flhA"
                     /locus_tag="ACHK49_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO9262409.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGADAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RHQLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLASMAPRLAEDLSAVLNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNLVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALS"
     gene            23935..24213
                     /locus_tag="ACHK49_001747"
     CDS             23935..24213
                     /locus_tag="ACHK49_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO9262410.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            24206..24562
                     /locus_tag="ACHK49_001748"
     CDS             24206..24562
                     /locus_tag="ACHK49_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO9262411.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(24619..25323)
                     /gene="yafL"
                     /locus_tag="ACHK49_001749"
     CDS             complement(24619..25323)
                     /gene="yafL"
                     /locus_tag="ACHK49_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO9262412.1"
                     /translation="MLCLPFSSFASATTSHITFSYAARQRMQNRARLLKQYQTHLKKQ
                     ASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHN
                     ITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAKLPRTANEMYHYRR
                     ATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSQLAEPFWQ
                     DHFLGARRILTEETIL"
BASE COUNT         5446 a         7063 c         6916 g         5967 t
ORIGIN      
        1 acgggtaaat aatgcatcac taaaaatacc aaatcaaggt atttttggta ttttggctaa
       61 tttttgcttt tatccttatt tttcaattgg ataaatagaa agaaaccttt ccttagaaaa
      121 gcaccaagag gtatctttat gtcgcaggga aaagaacctg ttattgaacg tattggaatg
      181 cttacaaagc gctacccttc aagaagtgca gctgccagag cgtggggaat caatatcaac
      241 acattaaaca gctatttcaa gggaggacaa acacaaccag cccccagaga aagtatcctt
      301 atacgaattt ctgaaagtga aggagttagt cttgagtggc ttaaagaggg caagggtgaa
      361 tcaccaaacg atgttggtat ttgtggtaat tttggttgtt ctggtgataa gggagatcaa
      421 cttacacaga tgctttcatt ccttacgaaa gacgaacgag aacagttaac caatctcctt
      481 gctcgcaaag gtgtagagac ggttctgaca ctgctggata aagaaaacca ggatttatta
      541 caactagaag gcaggaagcg cctggctgcg ttgatgattg tagatatgcc agacgattgc
      601 gtcagagaga ttttggaaaa aaccgagaca agtaggcaat caaatccggt agaatctaaa
      661 gcgggttagt ttggcgaggc tggcccgtat gttagttgcc atatagattt aatgaagttt
      721 taaacgcttt aaaaagttga gatcgcagta tcaaaagttt tgccgatttt agaataaacg
      781 attaattatt gcacaatcgg tatcaaatag actttaagcg ctcatccaga catcacctgt
      841 tttatttcag tatcttacag gtgatttcac ctcctttcac taaaaccctc gttggtatca
      901 aatgattcac ctggttacac gctgtcgcca ggaagaatcg ccgtcaccgt gccgtcctca
      961 ccaaacgcaa cattcgtcgc ccccgccgga acgtcgattt ccggctgcaa tggcaagccc
     1021 tgtgagttgg tcagtacgcc atcggcgttc acctgcaaat tgccgtcgcg ggtataggcg
     1081 atatcgccgt tggccttttg cacctgcaaa aatccctgcc ccataatggc gacattcatc
     1141 gcgttgtcgg tggtttcgac gttgccttcg gtgaaggttt tctgggtgcc gacaatgcgc
     1201 acgccgctac cgaattgcag cccggtcggc atgatgttgt tctggtcgag catcgcgccc
     1261 ggcgcttcct gggtctggta gaacagatcc tgaaacatca cccggtcacg cttgaagccg
     1321 gtggtgttaa cgttggcgat gttgttggca atcgcgctca tttcagcatc ctgcgccgag
     1381 aggccggttt tgctgatcca taatgctgcg ttcatggtgg taaattccgt gttatttttg
     1441 ggccgcggtt gccggatgcg gcgtgaacgc cttatccggc ctacgatgtg cggttacgag
     1501 ccgcgcaata agcggttgcc cgcgtcgctg atatcttcgg cggttttcat catcttgatc
     1561 tgtgcttcga actggcggtt catggcgatg gacgacatca tttcgcttac cgccgagacg
     1621 ttgctgccct cgagaaaacc gccgctgact ttgatgtttt catcgcgctg ggccggaacg
     1681 ccgtcggcag tcaccagcat cccttcagca tttttctcca ggttagccac cgggatatcc
     1741 accagcttca ggcgatcgat atccatcgtc gcggtcacat cgccgtcatc gggcgtcacc
     1801 gacagcgtgc cgtcgctgcc aaacgaagca atggcgttcg gcggcagaat gatcggcccg
     1861 ttatccccca gcaccagatt gccgtcgatg gtcagttgcc cctggtcgtc ctgctgaatg
     1921 ttgccgttgc gggtatagac ttcgttaccg tttttatcct gcaccgcaat gtaacccgcg
     1981 ccctgaatcg ccacgtccag cgggcgttcg gttttctccg caacacccgt gctgtcgttc
     2041 acgccgctgg ccccttcctg cgccatatag cgcgtgtcga agccgccgcc tttcaccttg
     2101 tcgttggttg ccattgccat atcggcgcga aaaccattgg tgttgacgtt cgccaggttg
     2161 ttggcgctaa tttgctgctc atacagggtc tgagaagcac cgctcagggc ggtataaatc
     2221 aggcggtcca ttacattgcc tggaacagcg cgtcatcgag ctgcgtgctg gtggcgataa
     2281 ctttggtgtt ggcctgatag ttacgctggg cggtcatcag gttgaccagt tcgctggtga
     2341 tatcgacgtt agagctttcg agcacgcccg acgacagcgt accgcaggtg ccggagccgg
     2401 gaacgccaat cagcggggtg ccggattcgc ccgtttgtac ccatgcggtg ccgctcactg
     2461 cctccaggcc gttttcattc gggaaagtcg ccagcaccac ctgcccctgc aacatgcgct
     2521 cgccgttgct gtaggtggcg taaactttgc catcgtcatc aacctgaaca ccgttttgcg
     2581 tggcggaggc gtagccgttg gcggcgttgg tggtgaccga aaattcagag ccgtattgcg
     2641 tacaggtgga gtaatcgacg gttaagtcga cgggcgcggc ggcgtcggtt tgaaattcaa
     2701 tggtctgcgg cgtggttgga gaggtcagct tcccggtgtt cggatcgaag gttaacgtcg
     2761 tcgccggaac gccggtctgc tgctgaccgt cgaaggtgta ctgcacttcc caggtgttgt
     2821 cgctggtttt ggtgaagtac tggcataccg aatgttcgtt gcctaatgag tcatacaccg
     2881 tggtggtgta gctgtcggtg tacgaactgc tgttagtcgc gtcgaacgga acggtggtgc
     2941 gatcgatagc ttcatcgctg gcgttgaagt tagcggtaaa ggtcaggctg ctgctggcct
     3001 gcgccgggat attgccggtt ttgatctgga tatcggtgac cgtaccggtt tgcagagtgc
     3061 cagtgtcatc gaccggatag ccctgcaaat aagcgcccga ggcgttgacg atatagccgt
     3121 ttttgtcggt ttcaaacgat ccggcgcggg tataggagat attgcctgcg ctgtcacaca
     3181 tgacaaaaaa tccgtcgtcg ttaatcgcta aatcgagcgc attgccggtg gagaccagcg
     3241 aaccgccacg cgaaatactt tgcgcggtgc ccgccacgct gacgcccatc gcctggcttc
     3301 cggcatacat ggcggaaaac tgggtggtca tcgacttata gcccaccgtt ccggcgttag
     3361 cgatgttgtt actgatcccg tccagctgtt cgttaacggc attcagcccc gttgcggcaa
     3421 tttcataact cattgtgtct tcctgttaat tgattcttac gccacatcac tgtcggcatc
     3481 gggtacgcca aactggctaa tcatggtgaa cggcacttcg cccacgcctg caacgttaag
     3541 caccggggaa ctgccgtcga gcgggatgcg cacatcttcc acttcgccgg agacttcaat
     3601 cggcacctct tcttccccgg tattggtcac caccgagacg tcgtaatcgc ccggcgggat
     3661 gccgtaatcc gcgggattca ccgagaaatc aacctggcct ggtccgacgg acgaactgcc
     3721 ttctgggatc agcgagacgg tgtagtcatc gcctgcggca tcggtaaagt ggagatcggc
     3781 ggtggtacag gcgtcgtcca gcgtagcgcg accgttaatg gtctggtcgc tcacctgcaa
     3841 ggcggtggtc tgcaccataa tgtcgtcgcc caccaggtta ccgagcgcca tcacctgaat
     3901 gttgctcatc agcaccgcgt tggtgttggc ctgcacgctc atctcttcca tcatcgccac
     3961 ctgcgccatc gacgacagct ggtcgatgta ttcggtagcg tcggtcgggt cggtcgggtc
     4021 ctggttttgg atctcggcga caaacagcgt caggaaggta tcgacaggcg agctatcgct
     4081 gctgctttgc gtgtcgctgg ccgccacatt attgctttcc gccgtggcgg tcggctgcgg
     4141 cgattgcgca ttcagcgcca gggtattcat caggcttctc ccagtttcag caaactttgc
     4201 tgcatacttt tcacgttgtt gagcacatcg acgttggttt cgaaatcgcg cgaggccgac
     4261 atcatgtccg ccatctgctc caccacgttg acgtcgggat accagacgtc accgttggcg
     4321 tcggcgagcg gagaatgcgg ttcatagcgt ttcaccgccc cgccggtttg cagcacgtcc
     4381 tgtacctgca cgctggcacc gtcaatggca tgacgatgtg tacccgccag caggctgtgg
     4441 tgatacaccg cggcaaacac cgggctgcgc gctttatacg cctgcgcctc gctgctggcg
     4501 ggcgcattgg cattcgccag gttactggca acggtattca gacgtagcgt ttgcgccgtc
     4561 atcgccgagc cggaaatctg atagatatca gtaaatgaca tgcttatttc ccttcaatgg
     4621 cctctctgat gccgctgatt tgcagattca gaaaggtcaa actgctttga taatccatac
     4681 tattttgtga aaaccgcgcc tgctcgctgt tcagttccac ggtgttgcca tcttccgacg
     4741 gctgcatcgg cacgcggtac tggacatcca ccgccgcgtt atttgcccgt tgcatctccc
     4801 tggcaaaatc aatatcttta gccttgaaat ttggcgtatc gacgttcgcc agattcgcag
     4861 tcagtagctc ggtcctctca agacgtaact tcactgcctg cggatgtact cccaatgcct
     4921 gctgaaaact gatccccatt tcctgcatca ctcctgcttc aatccgcata cgctaagcaa
     4981 tcgccatgcc attttttaag tcactgatga gaaagggttt atgagttttc aattttgcag
     5041 gcggaagttt tacttcctcc tggcgctggc cttaccgggc tacgccgccg tccatcccgt
     5101 gcaacacagc gcgcgggaac aagtcaacgc ccaggtgctg aatgccgcga gccaaaaaat
     5161 tgaatcgctg gcgcagcagc ggcagtggca tgactatcgc tacaccttta agatctacat
     5221 tccctcgcag attgcgaccg ccgcgccctg tactaaaaca cccggcgtca cgctgacttc
     5281 gcctgcggaa atcgccctca accggatgaa cttcaccgtt agttgcccgc aaagctggca
     5341 gatgaacgtg gcggtgcgcc cggatgtact ggtgccggtg gtaatgacga agtcactggt
     5401 tgcccgcgat atgccgctca ccgcgaatga tgtggagctt aagccctaca acgtcagcgc
     5461 ccagcgccgc gatgtgttga tggagcttga tgacgcgatt ggtttaagca gtaagcacgc
     5521 attgcaacca ggcagaccca tcaccaaaga agaactggtg tctccggtgc tggtcgggcg
     5581 cgatcagccg gtgatgattg tttaccagag cgcggggatc accgcctcga tgcccggcgt
     5641 ggcgctgaaa aatggtcgta agggcgagat ggtgaaagtg cgcaatgcca gcagccagcg
     5701 ggtcatcagc gcgatggtgg cggaaagcgg cgtggtgaca acggtaagcg cggagtaatt
     5761 tgcgcaggcg attaaagttt tgccgcctgt taaccgcttt accgcagtaa cccacccaaa
     5821 taccgagacg aggttgcgat gaaaatcacc ccgactatgc ctggcaatcg cccgacagcc
     5881 acaacggcag gctccagcca gccgcgcaaa accacggtga ccaccaccac gacgctgagc
     5941 gccgacgaca ttactcaggc gggattgcag tctgcccagc aaacgctgaa cgaccagcaa
     6001 gagagcgata tcgacagcaa caaagtggcg caagtgcagg cgatgctggc gtccggcagc
     6061 ccgcaagtgg acactgaaca gttagccagc gacatgctga gttttttcca gaactaagag
     6121 gccgatgaag tgagtatccg gttacaacag gtgaaggcgt tgttgcaggg cattcgtgac
     6181 gatgacgcgc tgtatgattc cctgcgcgaa cttctccagc gtcagcggat ctgcatgatt
     6241 cgccgcgcca gcgaagagtt gctggcggta aacgatgaga taacccatca ctatgagcag
     6301 ttgcatggac atagccatca gcggcatagc ctgctgaaaa tgctcggtgt tagcgtaaat
     6361 cgtgacggtc tggcgcaggt tttcacctgg cttccggctg ttcaaaaagc ggctgcacaa
     6421 cagttgtggc aacgccttga gcaaaaagcg gaacgctgca aagcctacaa cgacaaaaac
     6481 ggcgagctgt tgattcgcca gtatgaattt attcagtcgt ttttaggcag cgaagcagat
     6541 tttctctatc aggaataacg cgaatacctt ccccatagcg gaaatggatt ttccgcctga
     6601 tttccaatcc cttgttttta attacaaaaa tccccggcac gcttattgcg tatcctgcac
     6661 agcattaaag actggaagcc acctatgagt tttattgaat gttacgaacc ggaatacgtg
     6721 cgcaaatttt tagcgcgtca ccccggttcg cctttatacg tccgcaaggt gtggaaaaac
     6781 gagattcgcc tgagcctgtc attgaccgat attgccagct gcgaagcggt gctcaacgat
     6841 gtcagggcct ttgatttaca gctcacctac cgccctgtgg aagataattc cgcagagctt
     6901 tccgcgcgcg atgcgatagt aaaccaggtg attttgagta ccctcacgct ccgtgattta
     6961 accccggagc tttccctgta tgctgtcggg attttgctct cctgcgccag caaaatgccg
     7021 ggcagagacg gagacatcct ggcccgtttg acgacgctgc cacaggcgct ggccgatcat
     7081 gcgaaaaaag gtacgctaca ggcacagtac gcccagctcc cgcccgtgcc acagctggcg
     7141 cgccagttga tgacgctgct cggcggttgt gcgtttgact ggtcgatttt gccggaaagc
     7201 ccgcgcaaag cctcgctgcc gttgcaggtt actttactga cgctgcatga cgccaacagc
     7261 gaagccctgt tacaacagca gcttcagacg cagtggcaaa ccacctggca acagcatttt
     7321 gccaccgcgc cctggatgat gcgtaactgg ctgatttacc gcgtttacca cgatatgatt
     7381 gggcagactg acggggcgga ttatttcccg ctggtgtgcg atttttatct cctccgcacg
     7441 ttaatcagtt tatggacgct ggatggttct ccgctgcggc aggaggatat tttcgccctc
     7501 tttgccatgt ttgaacgctg gcgagccagt gaaaatgcgc tgttggttcg ccagcaaatt
     7561 cagtcgttat gtgccgccga cccgctgctt tcagcgtttt cgctgctaac gtgagtcgct
     7621 ctgcgcgcgc tttgcccctc accctaaccc tctccccaaa ggggcgaggg gaccgatcga
     7681 gcacggtgtt aaaatcggcg ctgattttct ccctctccct gtgggagagg gtcggggtga
     7741 gggcatcagc gcgcacgttc atcacattca cacattaact cacccaaaca ccatgccaac
     7801 accaaccatc atcaaaacac tcgccaccca ccgcgaaaaa ccgtttgtca gcgtcgtcac
     7861 gccaacctgg aaccgcggcg cattcctgcc gtacctgctc tatatgtacc gctatcagga
     7921 ttacccggca gaccgccgcg aactcatcat cctcgacgat tccccacaaa gccatcaaca
     7981 catcatcgac cgcctgacca atggcacgcc ggaagccttt aacatccgct acatccatca
     8041 cccggaaaaa ctgccgctgg gcaaaaaacg caacatgctc aacgaactgg cgcgcggcga
     8101 atacatcctc tgcatggatg atgacgacta ctatccggca gataaaatct cttacaccat
     8161 cgctatgatg caaaaacacc gggcgctcat ctccggttcc gaccagatcc ccatctggta
     8221 cagccatatc aaccgtatct ttcaaagccg cagctttggc ccacataata tcctcaacgg
     8281 caccttttgc tatcaccgta actacctgaa aaagcaccgt tacgacgatg attgcaattt
     8341 aggcgaagag aaaagtttca ccgacaattt cagcgttaac ccgctgcaac tgcccggcga
     8401 gcgcacgatt ttgtgtatct cgcatagcca caacaccttt gataaagact ttattatcgg
     8461 ggccagcacg ccggtaaaca cggcgcttac ggatatcgtc cgcgatccgc tgctgcgcaa
     8521 tgcttatctg agtctgcata acgccactca tcaccaggcg atcaaccatc aggcgatcga
     8581 tcaggtggtc gtcgtgaatc tggataaacg cccggatcgc ttacaacaga tccgtgaaga
     8641 actggcattg ctgcatattc cacccgagaa gatcacccgc cttgccgcca gtgaagatga
     8701 aaacggtcaa cggggccgcc gtcagtcgca cttgcaggcg ctgcgtctgg cgcaacagca
     8761 cggctggcaa aactatctgt tactggaaga tgacgcggtt attctcaagc aggagaaaca
     8821 tattcaggta ctgaacacgc tgctggcgag cctggcgaaa attccctggc aggtgatgat
     8881 cctcggcggc gaaatttcgc aaggaacgat gctaaaaagt ttgcctgggc tggtgcacgc
     8941 gcgggactgc cgcaaagtgt gcgcttatct ggtgaacagc cgctactacc cgcaactggc
     9001 gcagcagatg agcaacgatg agcactcgct ggaagactgc tggcaaccgc tgctacgcgc
     9061 cgacaaatgg ctggcctgtt acccgagcct ttgttatcag cgaccgggct ttagcgatat
     9121 ccagaaaaaa acgactgata atattgctcg ctatttcaat aagttacccg taaccaccaa
     9181 gccatccgcg ttacccatcg ccgacaccat cggctttttt atggaaacct cattccacta
     9241 cgcgctgtac cgcccaatca tcaccgccct acaggcgcaa gggctgagct gcacgctggt
     9301 gatcaacgac cgcgtgttta aaccgtttct ggatgagatg ctggaaacac tgaaaaacat
     9361 cgacgatccg cagcttaaag gtatgcgtct gtcagaaatg cagacacatg gtcagcgggt
     9421 gaaatgcctg gtcagcccct accacacgcc tgcgctcaat ggcctggcgg cggtgaacat
     9481 ccgcgctatg tacggcttag ccaaagagac gtggaaccac gccgactgga atcgttttta
     9541 ccaaagcatt ttgtgttata gccactacag tcagcaggcg cttgcgcatt tcggcagcgc
     9601 aaaagtggtc ggcaacccgc gcttcgacgc ctggcataac ggcacgttcg atcgcgcgtt
     9661 gccggaaaac atccaatcag attaccgtaa acctacggtg ctctatgccc caacgtttgg
     9721 cgcattaagc tccctgcccc actgggctga aaaattgggc cgcttgagcg gtgatgtgaa
     9781 cctgatttgc aaactgcacc atggcacctg ctcacgcccg gaagaagcag catcgctggc
     9841 cctggtgcgc aggcatttaa aacagcgcac cgactccgcc cgccacacgc tggcgctgct
     9901 ggcaaaagcc gattacgtgc tgaccgataa cagcgggttt attttcgatg ctatccacgt
     9961 tgataagcgg gtgatcctgc tcgactttcc gggcatgaat gatttgctcg acggtgagaa
    10021 aagttattcc acggcagaga gcgccgacca gcgaatccgc gaaatattgc cggttgctca
    10081 tgatgttgca gaactgcgtt atttgttgtc agaggcgttt gactgggggt cagtgcaggc
    10141 gcggcttacg gagattcgcc atcactattg cgatgcgttt atggatggca aggcgggaga
    10201 acgggcggcg attgtgattg tggaggcgct ggggtaaacc gcgcaactgc ctgatgcgct
    10261 tctcttatca ggcctacgga tcgatcacgt cctgtaggcc ggataaggcg tttacgccgc
    10321 atccggcaag aaaatacatc tctgttttag tactttataa aagccctcta atcacctcaa
    10381 taatccccgt cgtcgacacc gacggcgtgc gggggaaata gaccacctca caaatatacg
    10441 caaagctgtc aaacttccct gcccagtcat cccccatcac taaaatatcc gcactgtacc
    10501 cgcgcaaata ctccgctttc tgctctagcg attcttccag aaatacgcca tccacgcacg
    10561 ccagcccggc gatgatcgcc atgcggtcat cctgatggta aaccggtgcc ctgccctttt
    10621 tggcgatgtt cagggcgtcg gacgatacgc cgaccagcag ccgctcgccc aatgcgcggg
    10681 cgcgttgtaa aaggcgcaga tgaccgacat gaaacacatc gaaggtgccg aaggtgatga
    10741 ccgttttcat ggctactccg ctaatttgtg ccgcagccag cactgcacgg cgatgtcgtc
    10801 caccgctttt tgcgctttcg ccgtggcgct ttcttgttgc tcgcggcgtt tgtcgtccag
    10861 cgtcagggcg actactttct cttcgcacgc cgccgccgtg aggtttttct gcattcgcgt
    10921 ggctttgata ttggccagcg ttttttcctg ctcctgccag gcgattacct tgcgcagggt
    10981 gcctttgtag ccgctgacgt ttttcagcga ttccaccgac accgccgcac ccgcagatgt
    11041 tttctcgaca aggtagccta acgccttgat attgttgtca taacccgcgc aaatctgttt
    11101 ttgctttgcc agctcgacgg ttttgtcttt gaccgattta tcccgcaggc gctgcaactg
    11161 cgtcagggtg tcgatgattt gtcgcatagt tactgttctg cctttttgac accaggaaag
    11221 agaatttgca ggcggctttg caccagttcg agggaggcgg gttcgcgcat ctcctggcgc
    11281 aaaaatcgct cgatggccgg gaacattttt accgctttgt cgacgctggc gtcggcacct
    11341 gccacataac cgccgagcgg gatcagaggt ttgatctcca tataggcggc atagttttgc
    11401 tttaacgcgc gggcggattg ctgatgttcc aggctggtga cctggttcat gcagcggctg
    11461 atcgactggc cgatatcaat ggccggatag tgccccgctt ccgccagttt gcgggttaat
    11521 acgatatgcc cgtccagcac cgcgcgcgcg cagtcgacaa tcggatcttg ctggtcgtca
    11581 ccttccgcca gcacggtgta gatggcggtc atcgatcccg cgctttcgct gttgcctgcg
    11641 ctttcgacca gtttggggat catgccgaat gccgatggcg gataaccttt ggtggcgggc
    11701 ggttcgccga gcgagagggc gatttcccgc tgcgccatcg cgtagcgggt cagggaatcc
    11761 accagcagta agacgtgatg cccgcggtcg cgaaaccagg cggcgatgga gtggcacagt
    11821 tcggtggctt tcagacgcat cagcggcgat tcatcggcag gcgcgacgac gacgatagac
    11881 tttgccagcc cgtccgcgcc cagcgagtgg tcgataaact ctttcacttc gcgcccgcgt
    11941 tccccaatca gcccaaccac cacgatatcg gctttggtct ggcgggtgat catccccagc
    12001 agcacgcttt tcccgacgcc gctgcccgcc attagtccga cgcgctggcc tttgccgatg
    12061 gtcagcaggc cgttgatcgc tttcacgccc acgtccagcg gttcgtctac cgaacgacga
    12121 gttaagggat taaccgaggg cggcaacggc ggcagaaggt cgttgccgtt caggcggcct
    12181 ttgccatcca gcggttcgcc gaggccattc accacgcggc ctaaccagct ttcgcctatc
    12241 aggatgtcgt gggctttttc ttccgggaag acgcgtgcgc ctgccatcag gccgaccggc
    12301 tgtttaaacg gcattaaata ggtgatgtcg cgattaaagc ccacggcctg cgcgtcgatc
    12361 agcgtgtgat tggcactttc gatgcggcac agctgcccgg tcattagcgg gcagccgaca
    12421 ctctccagca ggatgccgtt aaggcgcacc agccgcccgg cgacgcgggc cagcgggatc
    12481 gactccagcg agcgcagggc gttatcgaac gcagaaaaat cactcaccct gcgactccgg
    12541 cgtgagtgtt tctttcagcg cggtcatgca ttgttcaagg cgatgctcgc agccgatatc
    12601 cagttcggat ttgtcggtga tcacccggca ttcgcccggt ggcaaatccg gcgaaggcgt
    12661 cagcccccat tcgctgacct tttccggcac cgcgtcgcgc aggcggttaa actcttccgt
    12721 gctcagcatt acttgcaggg tttccggcat cgccgggaag gccgccagcg cctcttccac
    12781 cagcgccagc aactgcgtgg gttgcagcgc cagttcacaa cggatcacct ggcgggtgac
    12841 tttttccacc agttgcagca aatcttcccg ctgtttacgt tcgatatgag cgaggaagtc
    12901 gttcacttta ccggtgatgg cttccagcgg ctgcgccgcc tcaacaaact gtttgcgccc
    12961 ttcctgctgc ccggcgaggc tgccttcggt gtaaccctgg cgtcgcccct cggcaaaacc
    13021 ttgctgatga ccttcgctaa agccctgctc ctggccttcg gtcattcctt gagcaaaccc
    13081 tttttgcagc ccttcctgaa atccgtccat caactggcgc tggtaatcgg cggcgctaat
    13141 actggtagtg agcggttctc cggtcaccgc acgctggcgc ggcgggaaac ggtgcaggcg
    13201 atagcgcccg cgcagtgttt caatggtcat ggttactccg ccgtctgttc cgcaaagagc
    13261 tgtagctgaa tttcgcccgc ctcttccagt tcacgggcga tgcccatgat ttcgcggcgg
    13321 atctgttcga tacggctgac cggcaccggc cccagacgcg cggtgatggc ttccagttgc
    13381 tgcgcctgcc gttttggcat taccgcgtaa atcgaacggc gcagcagggc ttcggtgcct
    13441 ttcagcgcca ccgcccagtc ttccatcggc acttcgtcaa gcagacgtcg acgcacctct
    13501 tcgttctggc ggctgaggat aaagaagtcg tacatctcat cctgaagctg ctccagcacc
    13561 tcttcgtcgc gctcgcgcat ctggtcgagg atcacctgct ggttgccctg gaagcggttg
    13621 acgatatccg ccgcctgttt gatgcctttc acttttgagc cgtgttccga aagcaccgac
    13681 aggccgcgtt cgatcagcct gtccagttca tccaccacgt cgcggttgac gtcgttgagt
    13741 ttcgccaccc ggtagagaat ttcgttttgc accgattcgt tgagatacga gagcaccgct
    13801 gccgagattt ccggcgtcag aaacgcgagg aacaccgcct gtaattgcag atgttcttcg
    13861 gagatcagca tcgccagctg gcgtggttcc acccattgca ggcgcgccat ccgcgagcgg
    13921 atctcatcgc cgtagatgcc gttgatcaca ctgctggcga tctcggtgcc gagggctttg
    13981 ttgagaatac cctgcaacat ggatcgcgat gcgccgttga tcccgctctg ttcgcggaac
    14041 tcatcaaaga agttattgat cactttgcgc gccatgctgg ttttcacgcc ggaaagccgt
    14101 gccatgtttt cgctcaggcg ccccacttct tcgcggctga gtttttgcat caccgtcgcc
    14161 gccgcctctt cgcccagaca cagtagcagg atcgccgcct gttccaggta gctgttgctc
    14221 ttatttgttg tcaattcgct cattgctggt gatccattgt ctgagaactt cggctacgcg
    14281 atcggtgtcg ctcatggcca gtttttgcag gaactcgagt tttacttcca ggccggaact
    14341 ctgagacggc aggctgtcat cgccgggcag cgacggcaga tcgacgtttt tgcgctcctc
    14401 ttccggcatg gcaatccacg gttcgctgac ggcttccgat tctggtgtga taatcggtgc
    14461 acgtaccgcc gtcagccgtt tcattaccgg gcgcaccacg aagaacagca acaacagcgc
    14521 cagcaggccg cagccaatca ggcgcaccca ggccagcacg ctgtcatctt tccacagcgg
    14581 aataattggt tcgaccggca ccgcctgcgg cacaaagtta agcagtgaaa gcgtcaggtt
    14641 atccccacgt tgcgcgtcaa taccggcggc attgttcagc aacgcggtaa gctgcgcggt
    14701 ctgctccggc ttccagtttt tcagcgccgg agcgttttga ttgagcacca ccgccacatt
    14761 gaggcgtttc accgcaaagc cgggatgctg aatatgctgg acgctacggt cgtaagagta
    14821 atcgcgcttg ctttcgctgt gtttggataa cgcttcaggc gagcgatttt cttccgtacc
    14881 gttagtcatt tgattcgccg caaccggcgg acggttgctg agagagccgg gcacacccat
    14941 cgccacctga ttggtgtcgc tgtccagcac gctctcttcg cggtttattt tcggcgcgtc
    15001 gccgtagtgt tcctgagttt cgtcgatggt gctgaggtcg agatccggca tcacgctgac
    15061 acggtaattc ccgctgccga ccagcgaatc cagcacgttc gccacgctgg cgtgggtttt
    15121 gtcctgaatg tctttgagga tctgatcgcg tttacgggta gcggcagaga ccgcctcgcc
    15181 cgcgccgatg ccgtcggtca gcagatttcc cgcctgatca accacgctaa ctttgctggc
    15241 gtgcagcccc ggaatgctgc cggaaaccag atgcacaatg gcgttcacct ggtccatatt
    15301 cagcttcgcg ccgtagtgca gacgcaccac caccgaggcg ctgttttgcg gctcatcgct
    15361 gaccacaaac gagctttctt cgttgagcgc cagatgcacc cgcgcgctct ccaccgcgtc
    15421 cagcgacata atgctctgcg ccagctcccc ttcgaggctg cgtttatagc gcacgttctg
    15481 cacgaactgg ctgctgccaa gcacttcgtc tttgtccatc agctcgtagc cgctgggcaa
    15541 aatcgcctgc acgcctttgg cggcaagggt catgcgcgtt ttcgacagcg cctcttccgg
    15601 caccagtatc tgcccgcttt gcgggtcaat gcggtaggtg agcttttcgc cgtccagcac
    15661 ggtgacaatc tgcgacacgg gcagattttc ctggcgtccg tagagcgaga cgtagccgcg
    15721 attgccggtc cacagcacgc taacgataat cgccgtcgcc gccacggcaa gccccacacc
    15781 cgccatcagc gcccagcgtt tgttatctgc gagacgcagt cgaaacgccg gaaacgccag
    15841 ggttaacttc tttatttgtg cattcatagg gttagatcga catactcatc agatcgttaa
    15901 atccggtggc gattttgttg cgcacctgca ccagcgcgga gaaagagagc gacgcctgct
    15961 ggctggcgat catcgcacct gccagatcat cgcttttgcc catgtcgata gccgtttgct
    16021 gctgctccgc cacctgctgg aactggtcaa cgtgaccgag cgcgccctgc ataatgcggt
    16081 gaaacgaaac gtccgggtcg gtggaactga aggtcatggc gggcagcacc ggcgcctggg
    16141 cgttggcctg catccgctgc acgtcctgca tgatctgcgc ctgcatggtg ctgttgattg
    16201 tggtaatgct catttttaac ttccgggtca ggagaaaact tcgataccgt gcttgcgcat
    16261 ggaggccagt cgatagcgca gtgcgcgcgg ggtaatacct aacaggtcgg cgattttgct
    16321 gcggttgccc tgatatttac gcatcaggtc agcgatatat tgatactggg cgcttcgtcc
    16381 gtgctggcct aaatcgctgg tttcggcaat gtgtacggcg ggctgagcag gccattgcag
    16441 ttcgctgtgg tcggcaatgt cattttccgg caggcctaag gcgtccgggt aaatcacgcc
    16501 gtcgcgattt aaaatcattc cccgctgaat ggcgttctcc agctggcgca cattgccggg
    16561 ccagcggtaa ttcagcatcg cacggcgggt agattctgaa agggtgatat ttttcaccag
    16621 caccgtggag tattttttta taaatgactc tgccagcgga ataatatcgt tcagacgttc
    16681 acgcagcggc ggcatggtaa taggaataac cgccagacga taatagagat cttcacgaaa
    16741 acgcccggcg gcgacttcct gctcaaggtt tttattggtg caggcaatca ggcgaaaatt
    16801 gagtttaatt tgtcggttgc tgcctaatcg ctcaacctgt tgttcctgca atacgcgtaa
    16861 tattttggcc tgtaacgcca gcggcatatc gccaatttca tcgagtaata aggtgccgtt
    16921 attcgccagt tccatttttc ctggcacact ggcaattgcg ccggtaaatg cgcctttgtc
    16981 gtagccaaat aaggttgctt ccagcatgtt ttccgggatc gcagcgcagt tcacgccgat
    17041 atagggcgcg ttttcatttt caccaaaggc gactgtgtga atatatttcg ccacgcactc
    17101 tttgcccgcg ccggtttcgc cctgaatgag caccggcacg ttaaatgcgg cgacacgctt
    17161 tgccagagta aaagcgttaa tgctggaagc ggcagttgca ataagttcag acatagggtg
    17221 aacgatataa gtagcgacaa taatcaggat gttctgaagc gtatcaccgg cgggattaac
    17281 aagttcgaat taaacgataa taaaaacatt atttgtttat aaacaatgat ttatccatgt
    17341 aggccggata aggcgtttac gccgcatccg gcaattgtgc accggtgcct gatgcgatgc
    17401 ggcgcgtctt atcaggccta cggttcgtgc aatgtgttat gacgatgcct gatatctcgc
    17461 cctttaataa tttaattatt ttttcaccct attaataatt tgcccctttc acgccccctc
    17521 tatttaatac ctcaccttgt gtcactgccg gatttgcgtg cacaaaatcg cccataccat
    17581 tacagcagag aaagtaagcc atgctgaagt acagtaaaac gccaggcatc tttaaattag
    17641 aaggtaatcg gctcggacgc ccctaccatc gcctgccgac cctgtttaca gggaattttg
    17701 acgtgattga ttcgcatctg ggcagctact ttctcaaaaa gcatcgcagc aatatcaccc
    17761 taaaaaagat tggctgcgaa atggatatta ttaataaaaa cgctgaactg atggtttccc
    17821 aggtcgggca tctggcgttt gatattgatc gctctttgct gttaatgctg ctgggcaatt
    17881 tttatggtct ggagtcctct cttgaagagg cgaaagcgca taacgggctg ccgaccaaaa
    17941 ccgaaacgcg gttaaaaaac cgtctggcgc tggatatctg cacgctgatt tttaatctgc
    18001 aaacctccgg tattgcgctg aaattaaagc tggacagcag cacggtaatt acccactggg
    18061 cgtatcaatt aacctttacc cttgcgggcg atgaagagag ctgttttcgt attttgctcg
    18121 acgacgccca caccgatttt attttaaatc tgatccgcca cagcgagcac agtaaaccgc
    18181 agcaggtggc gaaatcggtc aataaaccgg ccctgattaa agagatcatc cgctccctgc
    18241 cgctgaccct gaacgtcaaa atcgccgagc tatcgatgaa tgtcgcggat ctcacgcaga
    18301 taaaagccgg ggacatttta cccatctcgc tgggagaaac gttcccggtg gcgattgggc
    18361 aatctgaatt atttagcgcc ctgattgtgg aagacaaaga caaactgttt ttgtcggagc
    18421 tggcgggcaa aaatgagaat agccatgagt aagcaagaag atattttagc cggggatttt
    18481 ggcctcaccg atgaagttgc gcctgtcgca aaatccgccg acgctgaaac gctggtcacc
    18541 cgcctggaag accgtttttc cgactcgatg acgctgctaa aacgcattcc ggtgacctta
    18601 acgctggaag tgtcatcagt agaaataatg ttagccgacc tccttaacat cgacgacgac
    18661 acggtgattg agctggataa actcgccggg gagccgctgg atatcaaagt gaacaatatc
    18721 ctgctgggca aagcggaagt ggtggtggtc aacgagaagt acggcctgcg cgtgctggaa
    18781 tttaacaccc gcgatatcaa cgacctggca ccatgaaacg ccgcactcaa ctgacgctgg
    18841 gccttgggct gctggcgctt gcgccggtgg cgttggccca gggcggcgat attgcgctgc
    18901 ttaacgtggt cacccacggc aacactcagg agtacagcgt caaaatccag gtgctgattc
    18961 tgatgaccct cgtcggtttg ctgcctacga tggtgctgat gatgacctgc tttacgcgct
    19021 ttattatcgt gctgtcgctg ctgcgccagg cgctagggct acaacaaacg ccgccaaacc
    19081 gcattctgat tggcattgcg ctctccctca ccatgctggt gatgcgcccc atctggctca
    19141 atatttacga ccacgcagta gtgccgtttg aaaatgatca gattaccctg accgatgcgt
    19201 taagcaccgc cgcgacgcca ctcaaacgct ttatgctggc gcagactgac aaaaaagcga
    19261 tggcgcaaat catgactatc ggcggcgcga agggccatgc cgccgaccag gatctctcga
    19321 ttgtggtgcc cgcctatgtg ttgagcgagc tgaaaaccgc cttccagatt ggctttatga
    19381 tttacatccc gtttctggtt atcgacctga ttgtcgccag cgtactgatg gcgatgggga
    19441 tgatgatgct gtcgccgctg atcgtttcgc taccctttaa gctgatgctg tttgtgctga
    19501 tcgacggctg gtcgctgacc atcggtacac tcaccaccag cattcgcggg ctggggctgg
    19561 gctgatgatg ctgaccatag acgtagccgc cgacattgtc gccagcggca taaaagtggt
    19621 gattttgctg gtttcggtgc tggtagtgcc gagcctgctg gtcggcctgc tggtgagcgt
    19681 gttccaggcg gtaacgcaga ttaacgaaca gaccctaagc tttttgccga ggctgattgt
    19741 cacgctggtg gtgctgggcg tgtgcggtaa atggatgatt atccagttgc atgatctctg
    19801 cattcacctg tttacccagg ccgcgttgct ggttcattga gatgcgcaca agcgacgtta
    19861 cgcaactgat ggatctggcg ctggggctgt ggtttccctt tgtgcgcatt atggcgtttc
    19921 tgcgctatgt gcctgtgctg gataacagcg ccctgaccgt gcgcgtgcgg attattttgt
    19981 cgctggcgct ggcaatcatc atcacgccgt tgatccccca tcctgttccc cacgacctga
    20041 tatcgctcaa cagcctgatc ctgacggtgg agcaaatttt atggggcatg ttattcgggc
    20101 tgatgtttca gtttttgttt ctggcgctac agctggccgg gcaaattctc tcgttcaata
    20161 tggggatgag catggcggtg atgaacgacc cgagcagtgg cgcatcgacc accgtgctgg
    20221 cggagctcat caacgtctac gcgatcctgc tgttttttgc catggacggg catctgctgc
    20281 tggtgagtgt gctgtataag ggctttacct actggccgat tggcaacgcg ctgcatccgc
    20341 aaaccctgcg caccatcgcg ctggccttta gctgggtgct ggcgtccgca tcgctgctgg
    20401 cgctgccaac cacttttatc atgctgattg tgcagggctg ctttggcctg ctcaaccgca
    20461 ttgcgccgcc gctgaacctt ttttcactcg gttttcccat caacatgctg gcggggctgg
    20521 tctgctttgc caccctgctc tacaacctgc cggatcacta tctgcatctg gcgaattttg
    20581 tgttgcagca actcgacgcg ctgaaaggtc actatggcgg atagcagcag cgaagaaaaa
    20641 acagagaaac cctcggcgca aaagctgcgt aaagcgcgcg aagaggggca actgccgcgc
    20701 tcgaaggata tggggctggc ggcgagcctg tttgcggcat ttgtggtgat ctccagcagc
    20761 ttcccgtggt atgccgattt tgtgcgcgag agttttatca gcgtgcacca gtatgcacag
    20821 gagattaata acccagaggt gatcggccag tttctgcgcc atcacctgtt aattctcggc
    20881 aagtttattc tcaccttgct gccgatgccc gccgccgcgc tactctcctc actagtgccc
    20941 ggcggctggc tgtttctgcc taaaaaaatc ctcccggatt tcagcaaaat cagcccgtta
    21001 aaaggcattg agcggctgtt ttccagtgaa catctggcgg aaacgggaaa gatgacggtg
    21061 aagtcggtgg tggtgctggt gatgctgtgg gtaagcctgc gcaataactt tgcctccttt
    21121 ctcggcctgc aggcgctacc gttcaaactg gcgatgaatg agggtttatc gctctacgcc
    21181 tgcgtgatgc gcaactttgt catcctgttt atcttctttg cgttaatcga cgtgccgctg
    21241 gcgaaagccc tgtttaccaa agggctgaag atgaccaaac aggagctgaa agaagagtat
    21301 aaaaaccagg aaggcaagcc ggaagtgaag gcgcgcgtgc ggcgtctgca acggcagttg
    21361 gcgatggggc aaattcgcaa agtggtgccg aaagccaacg tggtgatcac caacccgacc
    21421 cactacgccg tggcgttgca gtatgactcg tcgcgcgccg ccgcgccgtt tgtggtggca
    21481 aaaggcaccg atgaaatcgc cctctatatt cgccaggtcg ccgctgagaa tcaggtggaa
    21541 gtggtcgagt tcccgcgact ggcgcgctcg gtttactaca ccacccaggt gaaccagcaa
    21601 attccgtttc agctttaccg ggccatcgcc cacgttctga cctacgtcct gcaaatgaaa
    21661 cactggcgcg agggtgcaca gccgcgcccg gccctgaaca gacatatttc cattcccaaa
    21721 gaggtgctta aactggatgg cgaaaacaac taaatcgttc ctcgcgctgc tgcgcggcgg
    21781 caatcttggc gtgccgctgg tgatcctgtg tattctggcg atggttattt tgccgctgcc
    21841 gcctgcgctg ctggatattc tgttcacctt caacattgtg ctggcggtga tggtgctgct
    21901 ggtggcggtg tcggcgaaac gaccgctgga attcagcctg ttcccgacca ttttgctgat
    21961 caccacctta atgcgcctga cgctcaacgt cgcctctacg cgcgtggtgc tgctgcacgg
    22021 gcatctcggc gcggacgcgg cgggtaaggt gattgagtcg tttggtcagg tggtgatcgg
    22081 cggcaacttt gttgtcggct ttgtggtgtt tatcatcctg atgatcatca actttattgt
    22141 cgtcaccaaa ggggccgagc gtatttccga ggtttcagcc cgctttaccc tggatgcgat
    22201 gcccggtaag cagatggcga ttgacgccga tcttaatgcc gggttgatca atcaggcgca
    22261 ggcacaaacc cgacgtaaag atgttgccag cgaggccgat ttctacggcg cgatggacgg
    22321 ggcatcgaaa tttgtgcgcg gggatgctat cgccgggatg atgattctgg cgatcaacct
    22381 gatcggcggc gtctgtatcg ggatcttcaa atacaacctg agcgccgacg ccgccttcca
    22441 gcagtatgtg ctgatgacca tcggcgacgg gctggtggcg caaatccctt ccctgctact
    22501 ctccaccgcg gcggcgatta tcgtcacccg cgtcagcgac aacggcgata tcgcccatga
    22561 cgtgcgtcac cagttgctgg caagcccatc ggtgctctac accgctaccg gaattatgtt
    22621 cgtactggcg gtagtgcctg gaatgccaca tctgccgttt ttgctgttca gcgccctgct
    22681 tggctttacc ggctggcgga tgagcaaacg cccgcaggcg gcggaagcgg aagagaaaag
    22741 tctcgaaacg ctgacccgca ccatcaccga aaccagcgag caacaggtca gttgggaaac
    22801 cattccgctg atcgagccca tcagcttaag cctcggctac aagctggtgg cgctggtgga
    22861 caaagcccag ggcaacccgc tcacccagcg tattcgcggc gtgcgacagg tgatatccga
    22921 cggcaacggc gtgctgctgc cggagatccg cattcgggaa aacttccgcc ttaagcccag
    22981 ccagtacgct attttcatca acggcattaa ggctgatgaa gcggatattc cggcggataa
    23041 actgatggcg ctgccctcca gcgaaaccta cggcgagata gacggcgtgc tggggaacga
    23101 cccggcgtac gggatgccgg tgacctggat ccagcctgcg cagaaagcga aggcgctgaa
    23161 tatggggtat caggtgatcg acagcgccag cgtgattgct acccatgtaa ataagattgt
    23221 gcgcagctat attcctgatt tatttaacta tgacgatatt acgcagttgc ataaccgttt
    23281 ggcgtcgatg gcaccgcgcc tggcggagga tttaagcgcg gtgctcaatt acagccagtt
    23341 gctgaaagtg taccgggcgc tgctgaccga aggcgtttcc ctgcgcgata tcgtcaccat
    23401 cgccaccgtg ctggtcgcca gtagcgcggt gaccaaagat catattctgc tggcagccga
    23461 tgtgcgcctg gcgctgcggc gcagcattac ccatccgttc gttcgcaagc aggagctgac
    23521 ggtgtatacc ctgaataatg agctggaaaa tctgctgact aacctagtga atcaggcgca
    23581 acagggcggt aaagtgatgc tcgacagcgt gccagtggac ccgaatatgc tcaaccagtt
    23641 ccagagtacg atgccacagg tgaaagagca gatgaaagcg gcggggaaag acccggtgct
    23701 gctggtgcca ccgcagctgc gccctttgct ggcgcgttat gcaaggttgt ttgcgccggg
    23761 gctgcatgtg ctgtcgtata acgaagtgcc ggatgagctg gagttgaaga ttatgggggc
    23821 gttgagttga tgggggattc ttttccatgc tttgcagcac ctgacaccat taattgacgt
    23881 cacactgcaa agagactacg atgacaccat acaatgacgt catggattga tagtatgatt
    23941 aaggtcagcg cgctaggttc tcgtcataga aaaacattcg acgatgtaat gacggagccg
    24001 ccaaaatctg gaattaaatg ggatgatgtt gttgcactca ttaaagctgt gggtggaaca
    24061 ataaaaaata acaacggctc cagacgaaaa tttcagattg gaacgaccaa attcagcaca
    24121 catgagccac atccaaaaaa tgtgatggat aaaggcgcag tagcaggtct taaggagtgg
    24181 tttgttaact gtgtaggggt agattatgaa taaagcatcg aacattatca caattaaagg
    24241 tcaaccagcc gcagtaacat ttgaagccga tatcaacgca tttcgcggca agttcctcaa
    24301 tgtaaacggt tattgtgact ttgtagccac aagtattgag gcattatatc gtgaaggcga
    24361 aagcgcgctg gatgagtgga tggcggattg tgaagaggac ggtattgccc cttttcgtga
    24421 agaagaagag caaaaacgcc tgacgcttcg tgttcctcat cacattgatt cgcggctgac
    24481 catcgtcgcc agacaacatt ccatttcaaa aaaccagctc attgttgatg tcttagaacg
    24541 tgagttttca gcccagatgt aaacaggtcg gatgccgcgt ggcatccgac cttttgccag
    24601 ttcgtcaata ttacccattt acaaaatcgt ctcctccgtc aaaatccgcc gcgcgcctaa
    24661 aaagtgatcc tgccagaaag gttcagctaa ctgacttacc cgaatggttt ccccggtgcg
    24721 cggtgactca ataaactgac cgtcgcccag atacaccccc atatggtcgg caatctcgcg
    24781 gctgtggata tggaaaaata gcaaatctcc ccggcgcagg tcgttattcg ccacaatggt
    24841 tgcccggcga tagtgataca tctcattagc cgtgcgtggg agtttcgcct caaggatctt
    24901 gttgtaggca taaaaaacta acccgctaca gtcgaagcct tgatcgggcc gcgtaccgcc
    24961 ccagacgtaa ggcttgccaa gctgttgctc cagacggtga atcgccactt cggtgatgtt
    25021 atgcagatgg tcgctgctta agaaatggtt gttttccgcc agcgcctgcc agcgtctgtc
    25081 actcgccttg agcggcgcag gaaaccattc tggatgctgt tttatctgtt cgcggttgtg
    25141 ctggcgtagc gcccttctgc tttcggcatt gccttccaca atatagctgg cctgtttttt
    25201 cagatgggtt tggtactgtt ttaataaacg cgcacgattt tgcatccgct ggcgggcggc
    25261 gtagctgaaa gtgatatgtg atgtggtggc gctggcaaat gaagaaaacg gcaagcaaag
    25321 caacagtaat cccgacaaca caaaagaggg aatcgacggc aaggacatac tcaataatgg
    25381 ctgatcatgc at
//
LOCUS       DAZGLJ010000047        25126 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000047 DAZGLJ010000000
VERSION     DAZGLJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.047"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(104..772)
                     /gene="artM"
                     /locus_tag="ACHK49_001750"
     CDS             complement(104..772)
                     /gene="artM"
                     /locus_tag="ACHK49_001750"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO9262413.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(772..1488)
                     /gene="artQ"
                     /locus_tag="ACHK49_001751"
     CDS             complement(772..1488)
                     /gene="artQ"
                     /locus_tag="ACHK49_001751"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO9262414.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1495..2226)
                     /gene="artI"
                     /locus_tag="ACHK49_001752"
     CDS             complement(1495..2226)
                     /gene="artI"
                     /locus_tag="ACHK49_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO9262415.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2244..2972)
                     /gene="artP"
                     /locus_tag="ACHK49_001753"
     CDS             complement(2244..2972)
                     /gene="artP"
                     /locus_tag="ACHK49_001753"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO9262416.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3190..3705)
                     /gene="ybjP"
                     /locus_tag="ACHK49_001754"
     CDS             complement(3190..3705)
                     /gene="ybjP"
                     /locus_tag="ACHK49_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO9262417.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3831..4154
                     /gene="ybjQ"
                     /locus_tag="ACHK49_001755"
     CDS             3831..4154
                     /gene="ybjQ"
                     /locus_tag="ACHK49_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO9262418.1"
                     /translation="MQFSTTPTLEGQIIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4151..4981
                     /gene="amiD"
                     /locus_tag="ACHK49_001756"
     CDS             4151..4981
                     /gene="amiD"
                     /locus_tag="ACHK49_001756"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO9262419.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(4978..5991)
                     /gene="ybjS"
                     /locus_tag="ACHK49_001757"
     CDS             complement(4978..5991)
                     /gene="ybjS"
                     /locus_tag="ACHK49_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO9262420.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWTV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6090..7520)
                     /gene="ybjT"
                     /locus_tag="ACHK49_001758"
     CDS             complement(6090..7520)
                     /gene="ybjT"
                     /locus_tag="ACHK49_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO9262421.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYSTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(7531..8532)
                     /gene="ltaE"
                     /locus_tag="ACHK49_001759"
     CDS             complement(7531..8532)
                     /gene="ltaE"
                     /locus_tag="ACHK49_001759"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO9262422.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(8569..10287)
                     /gene="poxB"
                     /locus_tag="ACHK49_001760"
     CDS             complement(8569..10287)
                     /gene="poxB"
                     /locus_tag="ACHK49_001760"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO9262423.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(10420..11388)
                     /gene="hcr"
                     /locus_tag="ACHK49_001761"
     CDS             complement(10420..11388)
                     /gene="hcr"
                     /locus_tag="ACHK49_001761"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO9262424.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVATCRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(11400..13052)
                     /gene="hcp"
                     /locus_tag="ACHK49_001762"
                     /gene_synonym="priS"
     CDS             complement(11400..13052)
                     /gene="hcp"
                     /locus_tag="ACHK49_001762"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO9262425.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(13196..14095)
                     /gene="lysO"
                     /locus_tag="ACHK49_001763"
     CDS             complement(13196..14095)
                     /gene="lysO"
                     /locus_tag="ACHK49_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO9262426.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTSLAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(14590..15285)
                     /gene="aqpZ"
                     /locus_tag="ACHK49_001764"
     CDS             complement(14590..15285)
                     /gene="aqpZ"
                     /locus_tag="ACHK49_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO9262427.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            15711..17369
                     /gene="ybjD"
                     /locus_tag="ACHK49_001765"
     CDS             15711..17369
                     /gene="ybjD"
                     /locus_tag="ACHK49_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO9262428.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HSSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(17366..18322)
                     /gene="ybjX"
                     /locus_tag="ACHK49_001766"
     CDS             complement(17366..18322)
                     /gene="ybjX"
                     /locus_tag="ACHK49_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO9262429.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            18473..19588
                     /gene="macA"
                     /locus_tag="ACHK49_001767"
     CDS             18473..19588
                     /gene="macA"
                     /locus_tag="ACHK49_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO9262430.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            19585..21531
                     /gene="macB"
                     /locus_tag="ACHK49_001768"
     CDS             19585..21531
                     /gene="macB"
                     /locus_tag="ACHK49_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO9262431.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(21604..21828)
                     /gene="cspD"
                     /locus_tag="ACHK49_001769"
     CDS             complement(21604..21828)
                     /gene="cspD"
                     /locus_tag="ACHK49_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO9262432.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            21978..22052
                     /gene="yljB"
                     /locus_tag="ACHK49_001770"
     CDS             21978..22052
                     /gene="yljB"
                     /locus_tag="ACHK49_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO9262433.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            22151..22471
                     /gene="clpS"
                     /locus_tag="ACHK49_001771"
     CDS             22151..22471
                     /gene="clpS"
                     /locus_tag="ACHK49_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO9262434.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            22502..24778
                     /gene="clpA"
                     /locus_tag="ACHK49_001772"
     CDS             22502..24778
                     /gene="clpA"
                     /locus_tag="ACHK49_001772"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO9262435.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
BASE COUNT         6160 a         6392 c         6754 g         5820 t
ORIGIN      
        1 gcgtccacgc cgcatccggc acaggcaccg tgctgatgtc tgatgcgacg ctggcgcgtc
       61 ttatcagacc tacaaacccc cccggcgaat gtacgcagct acattaattt cgccgttcga
      121 atgccagtgc tttgcgctcg atcagacgca tcatcagcgt cagcaggccg ttgacgacca
      181 ggtaaataat ccctgccgca ccgaacacca ttacatcgta ggtgcgtccg tacaacaact
      241 ggctgtatcc catcacttcc atcagcgtaa tggtgtaagc cagagaggta cttttgaaca
      301 ccagcaccac ttcgttggaa taagaagaga gcgagcgttt aaaggcatac ggcagcagga
      361 tcgccagcgt atcttttttg ctcattccca gggcgctaca ggactgccac tgaccttctg
      421 ggatcgcacg aattgcaccg taaaacagct gcgtggtata cgccgcactg ttcagcgata
      481 acgcaatcag cgcacataac cacggttctg acaacaaatg ccacagtgcc ggatactcct
      541 gcaaagtcgg aaactggccc ggcccgtaat aaatcaggaa gatctgcacc agcagcggcg
      601 taccggtaaa cagcgtgata taaccccgca ccagccacac cagcaccggc gttttcagcg
      661 tcaggatgat ggtaaaaatc aatgccagaa tcagtgccac aatcagcgag gcaacggtta
      721 gcgtcaggct ggtgtgtagc cctttcatca gttcgggtaa atactcaaac attagctggg
      781 cctccgctca aaacgtgtcg cgcgcaggtc aatgcgtttg agaatgtact gactgagcag
      841 ggtgatcacc aggtaaatcg ccgccgccac aatgtaccag gtaaatggtt cctgggtacg
      901 agtagcgatg ctttttgttt gcagcattaa atcattcaca ctaatcaaac tgaccagcgc
      961 ggtatctttc agcagcacca gccactggtt accgaggcca ggcagcgcat gacgccacat
     1021 ctgcggcatc accagacgga aaaagatagc cgatttcgac agccccagcg cctgaccaga
     1081 ctcccactga cccaccggca ccgctttcaa cgcgccccga agcgtttgcg aggcataggc
     1141 ggcatacagc agtgacagag cgatgacacc acaaaggaac gggctaacgt cgaagttctc
     1201 aatgtccatc tgcactggga tctgcacgaa cccaagattg atagtgaagc catccgaaag
     1261 cgtcagcagc agctgcgagg agccaaaata gataaacagc accaccagaa tttctggcag
     1321 gccacgcaga atggttacca gcgctgaacc tgcccacgcg acaggacgcc attttgccga
     1381 ctcccatacc gcaaagaaca tcgccagcgc cagcccgaca atcaatgcac aaacggcaag
     1441 gccgacggtc atcccggcgg cgcttgctaa aggaaaaaat tcattcatca ggaattactt
     1501 ctggaaccat ttgttgtaga tggtttcgta agtgccatct ttcttcactt tttccagcgc
     1561 agtgttgagt ttctgctgca gctcagtgtt gccctgacgt accgcgatgc cgaggccagt
     1621 gccgaagtaa tctttatcgg tcactttgtc gcccaccgcc gccagtttcg ggttatcttt
     1681 cagccactca gtgaccactg cggtgtcacc gaagacgccg tcgatacgcc cgttttgcag
     1741 atccagtttt gcgttctggt agctgtcata cggaacggta gtgatttccg ggtgcttatc
     1801 cataatgaat ttctggtgtg tcgtcccgtt ctgtacgccg acttttttgc ctttcagctg
     1861 atcaacactg gtgtatttgc cttgctgacc cacaaacagg gcagagttgt catagtacgg
     1921 ggtggtaaac agcacctgct tttcacgctc cggagtgata tccatgcccg ccatcacggc
     1981 ttctacgcga cggaatttca ggcttgggat caggctgtca aacgcctggt tagagaaagt
     2041 gcaggttgca tcaatctctt tacacagcgc ttgtgccagg tcgacgtcaa aaccaacgat
     2101 ctggttgttt gcatcaatcg attcaaacgg aggataggat gcttcggtag caaaacgaat
     2161 ggtttcggca gctgtggcgg aaagactaaa acctgcaatt aacgcggcaa tcagaacttt
     2221 tttcattgtt gttatcccga atcttagtga gagagatagt ttttaaatgc ttcggtttgc
     2281 ggctcggtaa agcagctcgc gtcgccttgt tctacgatat gaccattttc catatacacc
     2341 actcggctgg cggttttacg cgccacttca acttcgtggg tgacgatcac ctgggtaata
     2401 ttcgtttctg ccagctcacg aatgatgctg acgatttgtg ccgtaatttc cgggtccagt
     2461 gcggcggtcg gttcatcgaa cagcagtacc tgcggttcca tcatcaacgc acgggcaata
     2521 gcaacacgct gctgctgacc accagaaaga tgcagcgggt aacgatcgct ataaggtttg
     2581 agacgcagac gttccagcag tttttctgca cgggccagcg cctgatcttt actcaacccc
     2641 agtacacggc agggcgcttc aatcaggttt tgctgcacgg tcagatgcgg ccacaggttg
     2701 tattgctgaa acaccatgcc aacgttacga cgcaaatcgc gaatcgcttt gtcagagggt
     2761 gttttggtga aatcgaaatg gttgcctgca atgttgagcg taccggagcg cggcatctca
     2821 agcagattga gtacacgcag cagcgagctt ttacccgcgc cgctggggcc aagtaacacc
     2881 agcgtttcgc cctgtgggca atccagcgtg atatcgaaca gcgcctgatg cgcgccgtag
     2941 aagcaattaa tgccgtttaa ttgaatactc attgacactc gtatactggc agtctgatag
     3001 ctattgaggt cgaagatagt acctttgaca gaataattat gcaatatttc tgctttaaaa
     3061 gttaaaagca aagcgcatta ttcaataaac atagcacaaa ataacggggg cggtggtcgg
     3121 cgagcataaa tgtcggcatt cctcacgaaa tgccggacaa tttacggggt ttattggttg
     3181 atcaaggcgt tagcgattct cgatggactg acggagcgta cccgccgtgg catgaacgct
     3241 gccgcctaaa tagcgtacat cgtcgatcac ccaacactgg ccttcctgaa tcattaacac
     3301 ttcatcctgc cagctctgat caccctgttt tagatccaca cgcagaggaa tatttcgcgc
     3361 atcacgattc gggattgttg aggcactggc aacacgggcg ctctctggca aggttgtgcg
     3421 actggagaat ggatcgttgg tcagtagttc ccgatggtta ttatcccggg aggcatcgct
     3481 aagcagtgtc gccagtttgt cgctcagata agggcgcaag gcggtgatgt cgttgctgcg
     3541 gtgcaaaatg cggtagtcat aaaattgctg ggccacgtta tccgggcctc cttcaacgca
     3601 aggaccactg cgtgtgccgt tatctttata agctggagtg actgtggtgc aggcactgag
     3661 gagcagtgcg caggggataa gcattgtcaa tttgctgtag cgcataatga tttccttata
     3721 agcgatcgct ctgaaagcgt tctacgataa taatgatatc ctttcaataa tagcgtatca
     3781 gtctgataat gctttagaga tcgaaggctt aacaaacaag gagatcgatc atgcaatttt
     3841 ctacaacccc aactctggaa ggccagatca tcgttgaata ttgcggtgtg gtgaccggcg
     3901 aagcgatttt aggtgccaat attttccgtg atttctttgc cggtatccgc gatatcgttg
     3961 gcggacgttc cggtgcgtat gaaaaagaac tgcgtaaagc acgggagatc gcctttgagg
     4021 aattaggctc ccaggcgcgg gcgctggggg ccgatgccgt cgtcggtatt gatatcgact
     4081 acgaaacggt cgggcaaaac ggcagtatgc tgatggttag cgtcagcggt acggcggtga
     4141 aaacgcgtcg atgagaagag tcttctggct gatcgctgcc gctctgttac tggcagggtg
     4201 tgcaggcgaa aaaggcattg tcgagaaaga gggatatcag cttgataccc gacgccaggc
     4261 gcaggcggcg tatccgcgca ttaaagtgct ggtgatccac tacaccgcag atgattttga
     4321 tagctcgctg gcgacactga ccgataagca ggtcagctcg cattatctgg tccctgcggt
     4381 accaccgcga tacaacggta aaccgcgcat ctggcaactg gtgccggaac aagaactggc
     4441 ctggcatgcg gggattagcg cctggcgcgg ggcaacgcgc cttaacgaca cctctattgg
     4501 cattgagctg gaaaaccgtg gctggcaaaa atcggccgga gtgaaatatt ttgccccgtt
     4561 tgaaccggca cagattcagg cgcttattcc actggcgaaa gatattattg cccgttatca
     4621 catcaagccg gaaaacgtag tggcacatgc ggatatcgca ccgcagcgca aagacgatcc
     4681 ggggccatta tttccctggc agcaactggc gcagcagggg attggtgcct ggccggatgc
     4741 gcagcgggtt aacttttacc ttgccgggcg cgcgccgcac acaccggtag ataccgcgtc
     4801 attgctggag cttttggcgc gctacggtta tgacgttaaa cctgatatga caccgcgcga
     4861 gcagcggcgc gtgattatgg cattccagat gcatttccgc ccgacgttat ataacggcga
     4921 agcggatgca gaaactcagg cgattgccga agcattgctg gagaaatacg ggcaggatta
     4981 gcgcggcagt tttccgtggt cgcgtagcca ggcggcagtt ttctcgatac cttcatccag
     5041 ggtgatgacc ggctgataac ctaactcttc ctgcgcacgc gtaatatcca gcgtaaagtc
     5101 aaaattcaac ttggagacgc cgtagtgggt cagcggcggc tcttttgctg acttgcggcc
     5161 taaacgctcc atgctgcggg cgatcatatc cagcatcggg taggggacgg aacgaatacg
     5221 acagtcaata ttcaactcgt cgatcagctt ctgcacgatg ctgcgcagtg tgcgatgctc
     5281 gccgttggtg atgttgtaca cacgcccgga aggtagctta tcgcaggctt cctggcttgc
     5341 cagccacatt gcgtgcacgg cattttcata gtaggtcata tcgaccagcg cactgccgcc
     5401 atgcggtaac agaatactgc cgtagtggtg catcatatgc gccagacggg gaataaagac
     5461 tttatcgtgc ggtccgaaca gactttgtgg gcgcagaata gtaaagcgcg tttgtggatt
     5521 cgcctgcgaa agcatattga tcacttcttc gctggctgct ttgctgcggg caaactcgtt
     5581 ggcgaagcgg tgagggcgaa aatcttcttt aatatcgcga tggtggtgat aatcgaagta
     5641 cagggagggg gaagagatat gaataaagtt acgcacaccc caggcgacag tccattcacc
     5701 caggcggcga gtggcgcgaa cgttagccag atcgaaagcc tgttgtgtcc cccagggtga
     5761 ggtaaagctg gagcagtgcc acagcgtatc aatgcccgcg agcatcactt tagcttgtga
     5821 tgaaaccagc tcggtcagat ccgccggaac aaactctgcg cccatttttt ccagcaattt
     5881 gcccattgcc tcgttgcgac cggtcgctcg cacgctgatg cctttctggc ataaaaactc
     5941 taccgcgttt cgacctaagc cgctggtggc gccggtaacc agtaccttca tatcaatcca
     6001 ctgttgttga gaaaataacg tgcgcattct tccgtgattt cccccatgat gcaatgggaa
     6061 acatgaaaga ataacgcagg ttttgtcgat taatctgtgc tttgttctgc cagtctggcg
     6121 atttgttttg ccattccgcg aaaaataaac agatgcgcgg ggatcatcaa taaccagtaa
     6181 aacagccccg gcataccgtg cggatgccag aaagcgcgga catcgatagt actatagtcg
     6241 cctttatctt ccaggctaaa acacagtcgt cccagccccg gcgctttcat gccaaataac
     6301 aacgtaagtt gtttttccgg ttcaacgaca atcactttcc agctatccac cgcatcgcca
     6361 gtctgtaaat attcgcgctc cgggcggcct ttcgccagct tatgaccgat cgcgcggtcc
     6421 atcaacgccc gtgtctgcca caaaatattg ccaaagaaat aacgctcttt accgccgatt
     6481 tggttcacta cctgccataa agcagcaagg ctggcggacg ttttaacggt aaaccccgcc
     6541 tgtttggcaa aataaccgta ctctggtcgc cagcgggcaa aggcctgagc gtcgtagccc
     6601 cagtcgctgg agttgaccag tttttcctcc tctttcaacg tgctacgtac cgcgtcatcg
     6661 aaagcgatca gccgttgtgg gatgagtgca cgtagcgcgg tatcatccgc cagcagatcg
     6721 tgtttcagcc cctgaatcaa cgccctggcg gtggtgggcg gtacggaagt aatcacattg
     6781 agaaaccaca ccgaaatcca gcgggtgggg agggggatgg ggatcaacca gcggcgctta
     6841 ccgctcaccg ccataaaatg ttcaaactgt tgctgataac tgagcacctc tggtccggcg
     6901 gcttcgaaga tgcggtgttc gctggccgga tgatctaaca acgccaccag atagtgcagc
     6961 aagttttcca gcgcgatggg cgtggtgcgt gaacgtaccc agcgtggcgg cgttaacact
     7021 ggcaggttgt agaccatatc gcgcatgact tcgaacgccg ctgaacctgc gccaacgata
     7081 attccggccc gaagttcggt cacaggtaca ttcgcttcac gaagaatgtc cgccgtagcc
     7141 tgacgagcac gcagatgatc cgactgctca tgtggcgggg cctgcaacga actgagaaag
     7201 attaattgct taactggtac ttcacgtagc gcatcgcgga cgttgagagc cacctggcgc
     7261 tcctgagcga taaaatcgcc gccttcgccc atgctgtgca ccagaaaata gacggtatcg
     7321 atatcctgca acagggccgg aaggttatcc ggccagctga gatcgacttt atggcaactg
     7381 acgtttgcca gttgcagctt tgcaagcctg tcgacatgac gtgccgccgc caggatctga
     7441 tgcccttgct ggctgagtgt gcgcaccaga tgctgaccaa tgtagccact ggcaccgaga
     7501 actaaaatgc gttgcggcac gtctctctcc ttaacgcgcc aggaatgcgc gccagtgggc
     7561 ggcgacttcc gccagttgtt cgcgcgagac gtcaagatgc gtcaccaggc ggacaatcgg
     7621 cgaggcgtta atcagcacgt ttctcgcttt catgtattcg cctaacgcgg cagcattttc
     7681 ttccccgacg cgaacaaaca gcatattagt gtcctgacgc atcacatccg cgcctgcttc
     7741 acgcagttgc tccgccatcc aggcggcgtt gtcgtgatct tcctgcaacc gtgcgacgtt
     7801 atttttcagg gcatacatcc cggcggcagc cagaatcccg gactgacgca tcccgccacc
     7861 ggccattttc cgccagcgga tggcacgttt aatgtaatca cgattaccga cgagtaatga
     7921 accgactggc gtcccaagac ctttcgacag gcaaatggtg aacgaatcac aatattgcgt
     7981 gatctctttc agttcgcagc cgtaagccac cacggcatta aagatgcgcg caccgtcaac
     8041 atgcagcgcc agattgcgct cgcgggtaaa ttcccatgct tctttcaggt attcacgcgg
     8101 cagcactttg ccgttgtggg tgttttccag actgagtaat ttggtgcggg cgaaatggat
     8161 atcgtcgggt ttgattttca tcgccacttt atccagcggt agcgtgccgt cggcagccgc
     8221 gtcgatgggt tgcggctgaa tactgcccag caccgctgcg ccaccggctt caaacagata
     8281 gttatgcgcg gcctgaccga caatatactc ttcgccacgt tcgcagtgac tgagcagagc
     8341 gaccaggttg gcctgagtgc cggtaggcag aaaaatggcg gcttctttac cggaaagctc
     8401 tgctgcatag tcctgcagag cattaacggt agggtcgtct ccgtaaacgt cgtccccaac
     8461 cggggcggcc atcatcgctt cgagcatggc gcggctcggt cgggtaacgg tatcactgcg
     8521 taaatcaatc atggcatgtc cttattatga cgggaaatgc cacccttttt accttagcca
     8581 gttcgttttc gccagttcga tcacttcatc gccgcgtccg ctgataattg cgcgcaacat
     8641 atacaggcta aaacctttgg cctgttcgag tttgatctgc ggtggaatgg ctaactcttc
     8701 tttggcgacc accacatcca ccaacaccgg accgtcgata gagaaggcgc gttgcagggc
     8761 ttcatcaact tcagacgctt tttctacacg gatacccgta atgccgcacg cttcggcaat
     8821 gcgggcaaag tttgtgtcgt gtagttcggt gccgtcagtc aaatagccac cagctttcat
     8881 ctccatcgcc acaaagccca gcacgctgtt gttaaagacg acaattttca ctggcagttt
     8941 catctgcact actgagagga aatcgcccat caacatgcta aaaccgccat cgccgcacat
     9001 ggcgaccacc tgacgttctg gctctgtcgc ctgcgcaccc agcgcctgcg gcatggcgtt
     9061 agccatcgaa ccgtggttaa acgaacctaa caggcgacgc ttgccgttca tttttagata
     9121 acgtgccgcc cacaccgttg gcgtaccaac gtcacaggtg aaaatagcgt catcggcggc
     9181 aaaatgacta atttgctgcg ccagatattg cgggtgaatg gctttctcgc tcggtttagc
     9241 taaatcgtcc agccctttgc gggcgtcgcg gtaatcttcc agcgctttat ccagaaactt
     9301 gcgatcggct ttttcttcca ccaatggaag caatgcacgc agagtcgact tgatatcgcc
     9361 gaccagtgcc atatccacct tgctgtgagc gccgatgctg gctgggttga tatcaatctg
     9421 aatgattttg gcatcggtcg ggtagaaggc gcggtaggga aattgcgtgc cgagtagcac
     9481 taacgtgtcg gcgttcatca tggtatggaa acctgacgag aagccgatta acccggtcat
     9541 tccaacatca tacggattat cgtattcgac atgttcttta ccgcgcaggg catgaacaat
     9601 aggcgcttta attttcccgg caaactcaac taactcttta tgcgcccccg cgcagccgct
     9661 gccacacatc agggcgatat tgctggaata acgcagcagt tgcgccagtt tgcgtaactc
     9721 ttcttcttcc ggcgtcacga ctggttgtgg cgcatgatac cagtgcgtgg ttgccccttc
     9781 tggcgcaggt tttaacgcca cgtcgcctgg taacacgaca accgaaacgc cacggttaag
     9841 caccgctttg cgcatggcaa tcgccagtac ttgtgggatc tgctccgggc tggaaaccag
     9901 ctcgcaatag tgactacatt cgcggaatag ctcttgtggg tgggtttcct ggaaatagcc
     9961 gctgccaatt tcgctggagg gaatatgagc ggcaatcgcc agtaccggaa cgtgattgcg
    10021 gtggcaatcg aacaggccgt tgattaagtg caggttgccg gggccgcacg atccggcgca
    10081 gaccgccagt tctccgctaa gttgtgcttc agcgccagcg gcaaaggccg ccacttcttc
    10141 gtggcgggtg gacatccact cgatggtgcc catgcgatta agactgtcac taagaccgtt
    10201 cagagagtcg cctgtgactc cccagatgcg tttcacccct gccgattcga gtgttttggc
    10261 gatataagct gcaaccgttt gtttcatggt tctccatctc ctgaatgtga taacggtaac
    10321 aagtttagtt catctgacgg agggggaagg gatgggagag aaaggaggca ctaacggtta
    10381 aatagcccga tgaaaggaat atcatcgggc ataaggcgat tatgcgagaa ccaaatcccc
    10441 ctgcggatgg caggagcagg ccagtacgta accttcagcg atttcggcgt cggtcagcgt
    10501 cattgtactg ctcaccgtat attcaccgga aaccactttt gtcttacagc agccgcaaac
    10561 acccgcacgg caggtagcga caaccggaac gttattgctt tccagcgcct ccagtagcgt
    10621 ggtgccaacc ggggcgtaaa attctcgtgc cggttgcagt ttggtgaatt tcagaccgct
    10681 ggtcgccgct tctgctactg gggtgaagaa tttctcttta aagaaacgcg tcacgccgag
    10741 cgctttcact tcctgctcta cccaatccat atacggagcc gggccgcagg tcatcacggt
    10801 acgtgaagct aagtcaggta cacctgccag cagttcgcga gtgagacgac cagcgataaa
    10861 gccttcggta acgttatttt ctgccaccag cgttaccgga tagttacgcc actcatcggc
    10921 gaaaataaca tcctgcggcg tacgcacgtt gtagatcacc cgcacatcgg cctgtggacg
    10981 gttcttcgca agccagcgac gcatcgacat aatcggcgtg acgccgcagc ctgccgccag
    11041 caacaggaat ttatcttctg ctttatcgtc gcaggtaaat tcccccatcg cgtccgaaag
    11101 ccagagataa tcaccgcgtt ttacatcgcg cgtcagccac tgggagccga caccgtcatc
    11161 aatccgccgc acggtcaggg tgatatattc actcacgcct ggcgtggagg aaatggtgta
    11221 agcacgcagc gtttccgctg agtttcgcac gctgaccagt gcatattgcc cggcgcgata
    11281 tgggtagtaa tcgtggcaaa tcagggaaat cgtccacaca tccggcgttt cttgcgtaat
    11341 gtgatgaacc tgcatccgcc acgggcattg attcgttggc atcgtcatcg acaaactcct
    11401 tacgcgctca acagttgctt catgtcttct tcaacagtgg tgatagaacg caggccaaat
    11461 ttctcgttca gcaccgccag caggtctggt gtcaggaaac caggtgcagt cgggccggtg
    11521 acgatatttt tcacgcccag agaaagcagc gtcagcagaa tgacgatcgc tttctgttca
    11581 aaccaggaga gcaccagcga cagcggcaga tcgttgacac cgcagcccag tttctctgcc
    11641 agagtgacag ccagaataat cgctgagtaa gcatcgttac attgacctgc atctaccaga
    11701 cgcggcagac cttcgatatc gccaaactca agtttgttaa agcgatattt accacaggcg
    11761 agggtcagga tcaggcagtc atccggcacg ctggtggcga aatcggtgaa gtagtggcgc
    11821 tcgccgcgtg cgccgtcaca gccaccaagc aggaagatat gacgcagttt ttcacggctc
    11881 accagatcaa tcagcgtatc agcagcgcca agcagcgtct ggcgaccaaa acccacggtg
    11941 ataaggtgcg gaatttcgct gtacgggaag cctgccatct gttgcgcctg ggtgataacc
    12001 gcagagaaat catcaccgtc cagatgacgc acgccaggcc agccaacaat gctgcgggtc
    12061 cagatacgat cgtcataagc gcctacggtt gggtcgatga tgcagttcga ggtcatcacg
    12121 atggggccag ggaaacgagc gaactccact tgctgattct gccagccact gccgtagtta
    12181 ccgaccagat gcttgaattt acgcagctcc gggtagccat gcgcaggcag catttcgccg
    12241 tgggtgtaga cattaacgcc cgtgccttcg gtctgttcca gcaggttgta gagatctttg
    12301 agatcgtgac cggaaatcag aatgcattta cccgccgtcg ctttgacgtt gacctgggtt
    12361 ggcgtcgggt gaccgtattt accggtttcg cctgcatcca gaatgctcat cactttgaag
    12421 ttcatctggc cgatttccat tgaacactca agaagcgcgt tcatatcggc aggccaggtc
    12481 cccagccacg ccatgatttt atgatactgg gcataaatat cgttgtcgta ttgaccgaga
    12541 acatgcgcgt gttccatata ggccgccgca cctttcaggc catacaggca cagcagacgc
    12601 aggccgagaa tgttttcgcc aatcgccgct ttatctttgt taggggtaaa ttctgcagcc
    12661 tgacgttgca gctcgccgag atcgtcgctc accagttgca ggtcagccat cgggttatcg
    12721 acgcgcgcgt tggcatctac agccaggcat tgcgctttca gcgcctcgcg cagggcaatc
    12781 gcttcacgag cgtagccgac aatacgcgga gaatcgaagt taacgttggt cagagttgag
    12841 aaaaaggcac gtggcgcgaa gctgtcaaca tcgtggttga tgatgccgta ttcacgcgct
    12901 tttaccgccc aggcagaaag cccttgcagc gccgcgatga gtaaatcctg aaggtcagaa
    12961 gtttccgccg ttttaccaca catcccctgc gcgtatgagc agccgtttcc tgccggagta
    13021 cggatagttt gttcacattg cacacaaaac atgatcacac cttttaaagt tatatttaat
    13081 atacatgttt aaggttaaga cgcttaacgc ggggataaaa gggatttttc atgcaacttt
    13141 aagggagatt gatttagcgc aattttggcg gcaggaatct accgccagag aggtattacg
    13201 cagagaaaaa ggcgatgagg atcggcacta acaggctaag aataaaaccg tgaacaattg
    13261 ccgccgggac catatccagc ccgccagtac gttgaagaac gggcagggtg aaatccattg
    13321 atgtggcacc gcataagccc agtgcagtag agcggctgcg gcgaatcagc ccagggatca
    13381 acataatagc aatcagttca cgggccagat cattaaaaaa cgccgcgctc ccgattaccg
    13441 gaccaaaaga ttcggtcaat aaaataccgg aaagagaata ccagccgaaa ccggaggcca
    13501 ttgccagcga ggtattgatg gggagatcaa gaataaaggc gttaattaaa ccaccaatta
    13561 atgaactgac aaccaccacc acggcgacaa tcattccccg gcgattaagg acaatctgct
    13621 ttaaggtcat gccattattg cgcaactgaa taccaacgag gaaaagtagc aaaattaacg
    13681 tgtattcact ggcttcggtc gcgtgttgta agaaagccag tccactcaga ccaatggcaa
    13741 aaccaatcac tactacgccg cacagtttta gcgactccag cgccatcgca atacgcgacg
    13801 ggagtttttc ttgctgatgg tggttgcgcc acggcaggcc tcgctccagc cacatcaggg
    13861 cggcaatatt acacagtaaa ataacggtaa tactgacggc agaataatgc agaatcgcca
    13921 acaggttact ggcgaggtta tcgaggaacg ccagacttat acccataaaa aagagaataa
    13981 ggtaaaccat ccagcttaat agctgattaa taacttttaa cgcagcttgt tggcgaagcg
    14041 gaatgaggta acccacaatc aggggaacca gaatgattaa cagcccagaa aacatgaaaa
    14101 cccagtcctt gcaaagatga agtcgaaatg cgcgatgaca cactactgaa agcggaagga
    14161 cgagtaaagt tgcaattaaa aggaaatgtt atgcataagg agcagtagag tattcgtttt
    14221 catttaaaga tattcttgcg ctttaattac aaactgcacc gatgttggtg gcgtcaaaat
    14281 cgccgaggcg ttccctgaag gccggggcag cccacatgga tgtgggctga gggcgcgttt
    14341 tacagggatg ttacctcgcg cccgacccgg tagccgtaag ggataagtcg agggcaccgc
    14401 gcagcggcga ttttgttcgc cagagcccgg gggtgcatgg ggcggcggcg attggccgcc
    14461 ccctgcgcgc tccttgcgcc agtggcaata tgtggcttag ctcatgaaag gagcgcaaca
    14521 agatgatgaa tcaacatata acaacatctt aaaaaaaggc ctgacattac gccaggcctt
    14581 ctgcgttaat taatcacgct tttccagcag ggtccggtaa atcagaccac cgataatgcc
    14641 gccgacaatt ggcaccaccc agaagaacca cagttgttct aatgcccagc cgccctggaa
    14701 gatagcaacc gcggtgctgc gcgccgggtt aacagaagtg ttagtcaccg gaatactaat
    14761 taagtgaatc agggttaagg ccagaccaat agcgatcggc gcaaaacctg ccggcgcgaa
    14821 tttgtcggtt gcgccgtgga tcaccaacag gaaacctgca ctcaatacca gttcaactac
    14881 cagcgcggaa agcatggaat aaccgcctgg tgaatgctcg ccataaccgt tagaagcaaa
    14941 accgctggct gccgcgtcaa aacccgtttt accactggca attaaataca gcagcgccgc
    15001 tgcaacaata ccgccgacaa cctgggcaat tacgtagcca acgacttctt ttgccggaaa
    15061 acgtccgcca gcccataaac caatagtgac cgccgggtta aaatgaccac cagaaatatg
    15121 accaacagca aaggccatcg tcagaacggt cagaccgaac gccaacgcca cgccggcaaa
    15181 accaatgcct aattccggga agcctgcggc cagtacagca ctaccacagc caccaaaaac
    15241 aagccagaaa gtaccaaaac attcagctgc taattttctg aacatatcca cctcaattaa
    15301 aaattgaccc tgtgaaaaat atggtcgttt tatagggtcg tcgtaaaaaa gtgacgacgg
    15361 aaataatgcg cggctatttt aaaaacgaag gcgagtcatt caccagataa ataaatccag
    15421 taaatttgat ttagggcaac agcgggttgc cccatatagt catttgtctg attgacagtg
    15481 tagtgcaagc aaaagattta atcctttagg cgtaataaaa aataatttat catgctaatt
    15541 atttgatttt gttgtttttg cagacttatc agcaagaggg agtataacgc gattattcgc
    15601 tcatttttca gacatttgcc atgcttaaat gtgatgtcat cacgtattag caaggccttt
    15661 cccgttatac tgccagcgta aaggataagt cacatatttc tggaggggat atgattcttg
    15721 agcgcgttga aattgtgggt tttcgcggta tcaaccgttt gtcgttgatg ctcgaacaaa
    15781 acaacgtcct gattggggag aacgcgtggg gtaaatccag cttgctggac gccttaaccc
    15841 tgctgctatc gccagaatca gatctctacc attttgagcg cgacgatttc tggttcccgc
    15901 cgggagatat caacgggcga gaacatcatc tgcatattat tttgaccttc cgcgaatcgc
    15961 tgccaggccg acatcgggtt cgccgttatc ggccgctgga agcgtgctgg acgccatgca
    16021 ccgatggcta tcaccgtatt ttttatcgtc tggaagggga gagtgcggaa gacggcagcg
    16081 tgatgacact gcgcagtttt ctcgataaag acggacatcc gattgatgtc gaggatatta
    16141 acgatcaggc acgccatctg gtgcgtttaa tgccggtgct gcgcttgcgt gatgcccgtt
    16201 ttatgcgccg tattcgtaac ggcacggtgc caaatgttcc caatgtggaa gtcaccgcgc
    16261 gccagctcga tttcctcgcc cgtgagttgt cctcacatcc gcaaaatctc tctgatggac
    16321 agattcgtca gggactttcc gcaatggtgc agttgcttga gcattatttc tctgagcagg
    16381 gggctggaca ggcgcgatat cgtttaatgc ggcggcgggc cagcaatgag caacgaagct
    16441 ggcgctatct ggacatcatc aaccggatga ttgaccgacc tggagggcgc tcgtatcggg
    16501 ttattttgct cggcctgttt gctactttat tgcaggcaaa aggcacattg agactggata
    16561 aagacgcgcg tccgttattg ctaattgaag atccagaaac ccgtttacac cccattatgc
    16621 tttcagttgc ctggcatctg ttgaatcttc tgccattgca gcgcattgcc accaccaact
    16681 cgggtgagtt gctttcgtta acgccggtag agcatgtttg ccgactggta cgtgagtcct
    16741 cgcgcgttgc cgcctggcgt ctggggccga gtggcttgag taccgaagat agccgacgca
    16801 tatcctttca cattcgtttt aaccgtccgt catcgctgtt tgctcgctgc tggttgctgg
    16861 tggaagggga aacggaaacc tgggttatca atgaactggc gcgccagtgt ggacaccatt
    16921 tcgatgccga agggatcaag gtcattgagt ttgcccagtc cgggctaaag ccactggtta
    16981 aatttgcccg ccgaatgggg attgaatggc atgtactggt cgatggcgat gaagcaggga
    17041 agaaatatgc cgctacggta cgcagcctgt tgaataacga tcgggaagcc gaacgagaac
    17101 atttaacggc gttaccggcg ctggatatgg aacattttat gtatcgccag ggattttccg
    17161 atgtgttcca ccgcgtggcg caaatcccgg aaaatgtacc gatgaatctg cgcaaaatta
    17221 tctcgaaagc gatccatagc tcttccaaac ccgatcttgc cattgaagtg gcaatggagg
    17281 ccggacgtcg tggtgtggac tccgtaccga cgctgctgaa aaaaatgttc tcacgcgtgc
    17341 tgtggctggc gcgcggtcgc gcggattaac cgcgaaacat cgtggccatt tgtggctgaa
    17401 tagcgtcgag catctcatag cgccgacggt attcagcccg ttttttactg gcgatttcgg
    17461 caatctcttt tcgtgctatc tgtgctggaa ggcggtaatg gcgttcagca tcacatacgc
    17521 cgccaaccga ttcccagaaa gcgttgtaat cggcatggat cttgccttct ttatcacgat
    17581 aacgcaggct gcggtaaata tgcgtttcat tgctgacggc aataatctgc tctacctgca
    17641 aacgttgggc aaacagacag gccgcttcca tcacgaggcg tttgggaaat agcccgtggc
    17701 aggctttcgt cgcattctgg atttcctgat gtgggatttc ccattttgcg ccttgcagtc
    17761 cgccaataaa catcgttctt ttcccctgat attcacacag ggtaaaagtg agctctgcca
    17821 gaggaatacc ttcgctgttg cggaacagga ttgtgctgtc accttcttta tccattgaga
    17881 tcatcatggt cagctcaagc gtgaactgct cgccgttttt gccttccagt ttagccagtt
    17941 gcagcccggg ggtattcaaa tataagctga attcttccgc cgacatacat ccgcggagta
    18001 acgcataatg gtaacgtaac gcctccagca attgcttacg gctaagattc gccgcaaggt
    18061 aagggcgatg cagacgcaca ggcagtcgcg gttggcgcgt taacaacaca ttgagattag
    18121 gccagtggga aagttcgttc atccactcaa cgcttaaacg cggcataatc aacgagcgca
    18181 gcaaaaattt ctggcgaaaa ctacggcgat gccagaattt acccggccga cactgtccac
    18241 gtgccagact aagaaaaagt gacaggctgc tgagagattc agatggcgta aaggtccgtt
    18301 cagttagctg cgacatattc atgaaatcaa tggttataca tgacgtcgat ttcaccattg
    18361 cgtatcttaa ccaaacatca atagtgtgat tactaacgta aattttaggg ttttgttgat
    18421 atttcgttga agttaatgac ccggattggc atatggagta ttcagaatat ttatgaaaaa
    18481 gcggaaaacc gtgaagaagc gttacgttat tgcgctggtg atagtcatcg ccggactgat
    18541 tacgttatgg agaattctta acgcacccgt gccgacttat cagacactga ttgtgcgccc
    18601 cggtgattta cagcaaagcg tgctggcgac cggaaagctg gacgcgctgc gtaaggttga
    18661 cgtgggcgcg caggtcagcg gtcagttgaa aactctgtcg gtggcgattg gcgataaagt
    18721 aaaaaaagac cagcttttag gggttattga tcctgaacag gctgaaaacc agattaagga
    18781 ggtcgaagca acgctgatgg agctacgtgc gcagcggcag caggcggaag cggagctgaa
    18841 actggcgcgg gtgacgtatt cccgtcagca acgtctggca caaacgcagg cggtttcaca
    18901 gcaggatctc gacaccgccg cgacggagat ggctgtgaaa caggcgcaaa ttggcaccat
    18961 tgacgcgcaa atcaagcgca atcaggcctc tctcgatacg gctaaaacca atctcgatta
    19021 cactcgcatc gttgccccga tggccgggga agtcacgcaa atcaccactc tgcaaggcca
    19081 gacggtgatt gccgcacaac aagcaccgaa cattctgacg ctggcagata tgagcaccat
    19141 gctggtaaaa gcgcaggttt ctgaagcgga tgtaatccac ctgaagccgg ggcaaaaagc
    19201 ctggtttacg gtacttggcg atccactgac gcgctacgag gggcaaatca aggatgtact
    19261 accgacgccg gaaaaggtta acgacgctat tttctattac gcccgttttg aagtccccaa
    19321 ccccaatggt ttgctgcggc tggatatgac tgcgcaagtg catattcagc tcaccgatgt
    19381 gaaaaatgtg ctgacgatcc ctctgtcggc gttaggcgat ccggttggcg ataatcgtta
    19441 taaagtcaaa ttgttgcgta atggtgaaac acgcgagcgt gaagtgacga ttggcgcacg
    19501 taacgatacc gatgttgaga ttgtcaaagg gcttgaagcg ggcgatgaag tggtgattgg
    19561 tgaggccaaa ccaggagctg cacaatgacg cctttgctcg aattaaagga tattcgtcgc
    19621 agctatcctg ccggtgatga gcaggttgag gtgctgaagg gcatcaccct cgatatttat
    19681 gccggtgaga tggtagcgat tgttggcgct tcaggttccg gtaaatcgac cctgatgaat
    19741 attctcggct gtctggataa ggccaccagc ggcacctatc gcgtcgccgg tcaggatgtt
    19801 gccacactgg acgccgatgc gctggcgcaa ctgcgccgcg agcatttcgg ctttattttc
    19861 cagcgttacc atttactttc gcatttaacc gccgagcaga acgttgaagt acccgccgtc
    19921 tatgctggtc ttgagcggaa acagcgactg cttcgtgccc aggagttgct gcaacggctg
    19981 gggctggaag accgtacaga gtattatccg gcacagcttt cgggtggtca gcaacagcgc
    20041 gtcagcatcg cgcgggcatt gatgaacggt ggtcaggtaa ttcttgccga tgaaccaacc
    20101 ggcgcactgg acagccattc tggcgaagag gtgatggcga tcctgcatca gctgcgcgat
    20161 cgtgggcata cggtgattat cgtcacccac gatccgcagg tcgctgctca ggccgagcgg
    20221 gtgatcgaaa ttcgcgacgg cgaaattgtg cgcaatcctc ccgccattga aaaagtgaat
    20281 gttgctggcg ggacggagcc tgttgtcaac acggtgtctg gctggcggca gtttgtcagc
    20341 ggttttaacg aggcgctgac gatggcatgg cgggcgctgg cagcgaataa aatgcgtact
    20401 ttactgacca tgctggggat tattatcggt attgcgtcgg tggtttccat tgtcgtggtg
    20461 ggtgacgccg ccaaacaaat ggtgctggcg gatattcgtt ctattggtac gaatactatt
    20521 gatgtctatc ccgggaaaga ttttggcgat gacgatccgc aatatcagca ggcgctgaag
    20581 tacgacgact taatcgccat ccaaaaacaa ccgtgggtcg cctcagccac acctgccgtc
    20641 tcgcaaaacc tgcgcctgcg ttataacaat gttgatgttg ctgccagtgc caatggcgtg
    20701 agcggcgatt attttaatgt ctatggcatg accttcagtg aaggaaacac ctttaatcag
    20761 gagcagctga acggtcgtgc gcaggtcgtg gttctcgaca gtaatactcg ccgccagctt
    20821 ttcccccata aagcagatgt ggttggcgag gtgattctgg tcggcaatat gcccgccaga
    20881 gtcattggtg tggcggaaga aaaacagtcg atgtttggta gcagtaaagt gctgcgtgtc
    20941 tggctacctt acagcacgat gtccgggcga gttatgggcc agtcgtggct gaactccatt
    21001 actgtcaggg taaaagaagg atttgacagc gccgaggcgg aacagcaact cacgcgttta
    21061 ctttcactgc gccacggaaa gaaggatttc tttacctgga acatggacgg cgtcttgaaa
    21121 actgttgaaa agaccacacg tactttacaa ctgtttctga cgctggtggc ggtgatttcg
    21181 ctggtggtgg gcggtattgg tgtaatgaat attatgctgg tgtcagtgac cgagcggacg
    21241 cgggaaattg gcattcgcat ggctgtaggt gcgcgagcaa gcgatgtttt gcaacagttc
    21301 ctgatcgaag ccgtactggt ttgcctggtc ggtggcgcgt tgggaataac actgtcactg
    21361 ttaattgctt tcaccttgca gcttttctta cccggctggg agattggttt ttcaccgttg
    21421 gcactgctgc tggcgtttct ctgctcgacg gtcaccggga ttttatttgg atggttaccc
    21481 gcacgaaatg cggcacgact ggatccagta gatgctctgg cacgagagta atttttgaga
    21541 taaaaatgcc agccgatcgg gctggcattt tgcctttagg atgtacacaa tgagacagaa
    21601 gagctatgcg actgccgctt ctacttcgac gggcacaata acactggcgt gattgccttt
    21661 tggcccctgg tggacatcaa actgaacgga ttgtccagct tttagcgttc tgtaaccatc
    21721 catctgaatg gtggaataat gagcgaaaat atcttcgccg ccgccttcag ggcagatgaa
    21781 accaaaccct ttggcattgt tgaaccactt aacagtaccc ttttccatgc ttcgacatcc
    21841 ttcgcaaatc ttatacaagt aagatggaat aaaccggggt cagagagggg gctgttcaaa
    21901 acctcgccaa ctctagaaat acaatttaga gaattagggc gagccgtcaa gcgtttgaca
    21961 ggggccaggg ggcaggtatg aatcaaaaat ttgaagcagt taacgctatt gacaggaatg
    22021 tgacagatgt cgctgatgcc aacgatagat gatagttatc tatcatgtgg agtagattgg
    22081 tcaggcaaat aagctcttgt cagcggcagg gcgttctgcc gataaccgta accgaagatg
    22141 ataactgaca atgggtaaaa cgaacgactg gctggacttt gatcaactgg cggaagaaaa
    22201 agttcgcgac gcgctaaaac cgccatctat gtataaagtg atattagtca atgatgatta
    22261 cactccgatg gagtttgtta ttgacgtgtt acaaaaattc ttttcttatg atgtagaacg
    22321 tgcaacgcaa ttgatgctcg ctgttcacta ccaggggaag gccatttgcg gagtctttac
    22381 cgccgaggtt gcagaaacca aagtggcgat ggtgaacaag tacgcgaggg agaatgagca
    22441 tccattgctg tgtacgctag aaaaagcctg aatgcaggca taaaaattgg gggaggtgcc
    22501 tatgctcaat caagaactgg aactcagttt aaatatggct ttcgccagag cgcgcgagca
    22561 ccgtcatgag tttatgaccg tcgagcactt gttactggcg ctgctcagta acccatctgc
    22621 ccgggaggcg ctggaagcgt gttctgtgga tttggttgcg ctccgtcagg aactggaagc
    22681 ctttattgaa caaaccacac ccgttctgcc tgccagtgaa gaggagcgcg acacacagcc
    22741 gacgctgagt tttcagcgtg tactgcaacg tgcggtcttc catgtccagt cctccggtcg
    22801 caatgaggta accggtgcaa acgttctggt cgctatcttt agcgaacagg agtcgcaggc
    22861 ggcatatctg ttgcgtaaac atgaagtcag ccgtctcgat gtggtgaact ttatctctca
    22921 tggcacgcgt aaagacgagc cgacacagtc ttctgatcct ggcagccagc caaacagcga
    22981 agaacaagct ggtggggagg aacgtatgga gaatttcacg acgaacctga atcagcttgc
    23041 gcgcgtgggc ggaatcgacc cactgattgg tcgtgagaag gagctggagc gtgctattca
    23101 ggttctctgc cgtcgccgta aaaacaaccc gctgctggtg ggggaatctg gtgtcggtaa
    23161 aaccgcgatt gcggaaggtc ttgcctggcg aattgttcag ggcgatgtgc cggaagtgat
    23221 ggctgactgt acgatttact ctctcgatat cggttctctg ttagcgggca ctaaatatcg
    23281 cggcgacttt gaaaaacgtt ttaaagcgtt gctcaagcag ctggagcagg acactaacag
    23341 catcctgttt attgatgaga tccacaccat tatcggtgcg ggtgcagcgt ctggtggcca
    23401 ggtcgatgcg gctaacctga tcaaaccgtt gctctccagc ggtaaaattc gcgtaattgg
    23461 ttcgacaacc tatcaggagt tcagcaacat tttcgagaaa gaccgtgctc tggcgcgtcg
    23521 cttccagaaa attgatatta ctgaaccgtc gatcgaagaa actgttcaaa tcatcaatgg
    23581 cctgaaaccg aagtatgaag cgcaccacga cgtgcgttat accgcaaaag cggtgcgtgc
    23641 agcggtagag ctggcggtga aatacattaa cgatcgtcat ctgccggata aagccattga
    23701 cgttatcgac gaagcgggcg ctcgcgcacg cctgatgccg gtaagcaaac gcaagaaaac
    23761 cgttaatgtg gcggatattg agtccgtggt ggcccgtatt gcgcgcattc cagagaagag
    23821 tgtttctcag agtgaccgcg ataccctgaa aaacctcggc gatcgcctga aaatgctggt
    23881 cttcggtcag gataaagcca ttgaggcgct gactgaagcc attaagatgg cgcgtgcagg
    23941 tttaggtcac gaacataaac cggttggttc gttcctgttt gccggcccta ccggggtcgg
    24001 gaaaacagag gtgacggtac agctttcgaa agcgttgggc attgagctgc tgcgctttga
    24061 tatgtccgag tatatggaac gccataccgt cagccgtctg attggtgcgc ctccgggata
    24121 cgttggtttt gatcagggag gtttgctgac tgatgcggtc atcaagcatc cacatgcggt
    24181 gctgttgctg gacgaaatcg agaaagcgca tccggacgtg ttcaatattc tgttgcaggt
    24241 gatggacaac ggtacgctga ccgataacaa cggacgcaaa gcggacttcc gtaacgtggt
    24301 gctggtgatg accaccaacg ccggggtacg tgaaactgag cgtaaatcca ttggtcttat
    24361 ccaccaggat aacagcaccg atgcgatgga ggagatcaag aagatcttta caccggaatt
    24421 ccgtaaccgt ctcgacaaca ttatctggtt tgatcatctg tcaaccgacg tgatccatca
    24481 ggtggtggat aaattcatcg tcgagttgca ggttcagctg gatcagaaag gtgtttctct
    24541 ggaagtgagc caggaagcgc gtaactggct ggccgagaaa ggttacgacc gggcaatggg
    24601 cgcacgtccg atggcgcgtg tcatccagga caacctgaaa aaaccgctcg ccaacgaact
    24661 gctgtttggt tcgctggtgg acggcggtca ggtcaccgtc gcgctggata aagagaaaaa
    24721 tgagctgact tacggattcc agagtgcaca aaagcacaag gcggaagcag cgcattaatc
    24781 tgattgttag gtaggttggt caagtccgta atctcgaaag aggttacgga ctttttgttt
    24841 atggggtgga ggaggttcag accctttttt aatgatgatg gtaagttgtt gataattagt
    24901 gctgcgggaa ggtaaggata aaaaagggtg ctgcaggaga atgggatggt tttgctttat
    24961 taacaacggg ctaaacgtgt agtatttgag ttcactgccg tacaggcagc ttagaaatcg
    25021 acggggtgcg gtaaaacctt tgcgaacgcg ttcactgccg tacagacaga taaaatgcga
    25081 aaaaaaagct cgcactttcg tacgagctct tctttaaata tggcgg
//
LOCUS       DAZGLJ010000048        24949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000048 DAZGLJ010000000
VERSION     DAZGLJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.048"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            258..1673
                     /gene="gltX"
                     /locus_tag="ACHK49_001773"
     CDS             258..1673
                     /gene="gltX"
                     /locus_tag="ACHK49_001773"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO9262436.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(1725..2117)
                     /gene="yfeD"
                     /locus_tag="ACHK49_001774"
     CDS             complement(1725..2117)
                     /gene="yfeD"
                     /locus_tag="ACHK49_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO9262437.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(2119..2478)
                     /gene="yfeC"
                     /locus_tag="ACHK49_001775"
     CDS             complement(2119..2478)
                     /gene="yfeC"
                     /locus_tag="ACHK49_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO9262438.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            2699..2774
                     /locus_tag="ACHK49_001776"
     tRNA            2699..2774
                     /locus_tag="ACHK49_001776"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2732..2734,aa:Ala,seq:ggc)
     gene            2814..2889
                     /locus_tag="ACHK49_001777"
     tRNA            2814..2889
                     /locus_tag="ACHK49_001777"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2847..2849,aa:Ala,seq:ggc)
     gene            3097..5286
                     /gene="pdeA"
                     /locus_tag="ACHK49_001778"
     CDS             3097..5286
                     /gene="pdeA"
                     /locus_tag="ACHK49_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO9262439.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGDGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(5336..6538)
                     /gene="nupC"
                     /locus_tag="ACHK49_001779"
     CDS             complement(5336..6538)
                     /gene="nupC"
                     /locus_tag="ACHK49_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO9262440.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            6874..8112
                     /gene="mntH"
                     /locus_tag="ACHK49_001780"
     CDS             6874..8112
                     /gene="mntH"
                     /locus_tag="ACHK49_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO9262441.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(8253..8579)
                     /gene="ypeC"
                     /locus_tag="ACHK49_001781"
     CDS             complement(8253..8579)
                     /gene="ypeC"
                     /locus_tag="ACHK49_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO9262442.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(8694..9950)
                     /gene="yfeO"
                     /locus_tag="ACHK49_001782"
     CDS             complement(8694..9950)
                     /gene="yfeO"
                     /locus_tag="ACHK49_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO9262443.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            10154..11119
                     /gene="glk"
                     /locus_tag="ACHK49_001783"
     CDS             10154..11119
                     /gene="glk"
                     /locus_tag="ACHK49_001783"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO9262444.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVYDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            11338..11664
                     /gene="fryB"
                     /locus_tag="ACHK49_001784"
     CDS             11338..11664
                     /gene="fryB"
                     /locus_tag="ACHK49_001784"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO9262445.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            11686..12933
                     /gene="fryC"
                     /locus_tag="ACHK49_001785"
     CDS             11686..12933
                     /gene="fryC"
                     /locus_tag="ACHK49_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO9262446.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKGLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            12948..14033
                     /gene="ypdF"
                     /locus_tag="ACHK49_001786"
     CDS             12948..14033
                     /gene="ypdF"
                     /locus_tag="ACHK49_001786"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO9262447.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            14033..15070
                     /gene="ypdE"
                     /locus_tag="ACHK49_001787"
     CDS             14033..15070
                     /gene="ypdE"
                     /locus_tag="ACHK49_001787"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO9262448.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            15095..17590
                     /gene="ptsP"
                     /locus_tag="ACHK49_001788"
     CDS             15095..17590
                     /gene="ptsP"
                     /locus_tag="ACHK49_001788"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO9262449.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILTRASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(17593..18450)
                     /gene="ypdC"
                     /locus_tag="ACHK49_001789"
     CDS             complement(17593..18450)
                     /gene="ypdC"
                     /locus_tag="ACHK49_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9262450.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(18463..19197)
                     /gene="ypdB"
                     /locus_tag="ACHK49_001790"
     CDS             complement(18463..19197)
                     /gene="ypdB"
                     /locus_tag="ACHK49_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO9262451.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFANKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(19212..20909)
                     /gene="ypdA"
                     /locus_tag="ACHK49_001791"
     CDS             complement(19212..20909)
                     /gene="ypdA"
                     /locus_tag="ACHK49_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO9262452.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            21286..22524
                     /gene="alaC"
                     /locus_tag="ACHK49_001792"
     CDS             21286..22524
                     /gene="alaC"
                     /locus_tag="ACHK49_001792"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO9262453.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(22589..22660)
                     /gene="ypdK"
                     /locus_tag="ACHK49_001793"
     CDS             complement(22589..22660)
                     /gene="ypdK"
                     /locus_tag="ACHK49_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO9262454.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(23016..23936)
                     /gene="lpxP"
                     /locus_tag="ACHK49_001794"
     CDS             complement(23016..23936)
                     /gene="lpxP"
                     /locus_tag="ACHK49_001794"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO9262455.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            24289..24531
                     /gene="yfdY"
                     /locus_tag="ACHK49_001795"
     CDS             24289..24531
                     /gene="yfdY"
                     /locus_tag="ACHK49_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO9262456.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(24608..24883)
                     /gene="ypdI"
                     /locus_tag="ACHK49_001796"
     CDS             complement(24608..24883)
                     /gene="ypdI"
                     /locus_tag="ACHK49_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFO9262457.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
BASE COUNT         6003 a         6131 c         6589 g         6226 t
ORIGIN      
        1 cgctgtgtgg aatcgcatta tagggagagt tcaaaatgag tcaacgcatt ttctaaagaa
       61 attgttcgtt cgtcgtaaat ttaagcaaga tgatcgcaaa acagaccgtg ttgcgcaatt
      121 tgtcaacgaa aacaataatg cgtaaggttg caacccgaac tacattgagg aatcaggcgg
      181 gagtgataga atatcgccca ctcaattttt ccaggatttg ccggttgtcg gcatctttct
      241 aaacgtaagg ccatttcatg aaaatcaaaa ctcgcttcgc gccaagccca acaggctatc
      301 tgcacgttgg cggcgcgcgt actgctcttt actcctggct ttttgcacgt aaccacggcg
      361 gtgagttcgt gctgcgtatt gaagacaccg atcttgagcg ttccacgccg gaagctatcg
      421 aagccattat ggatggcatg aactggctga gcctggagtg ggatgaaggt ccgtactacc
      481 agaccaaacg ttttgatcgc tacaatgcgg tgatcgatca gatgctggaa gagggcactg
      541 cttataaatg ctattgctct aaagagcgcc tggaagcgct gcgcgaagag caaatggcga
      601 aaggtgagaa gccgcgttat gacggtcgct gccgccacag ccatgagcat catgctgatg
      661 atgaaccgtg tgtcgtgcgt tttgctaatc cgcaggaagg ttctgttgtt tttgacgatc
      721 agatccgtgg tccgatcgag ttcagcaacc aggagctgga tgatctgatt atccgccgta
      781 ccgatggttc cccaacctat aacttctgtg tggttgtcga tgactgggat atggaaatca
      841 cccacgttat ccgtggcgaa gaccatatca acaacacgcc gcgccagatc aacattctta
      901 aggccctgaa agcgccggtg ccggtttacg cgcacgtttc tatgatcaat ggcgatgacg
      961 gtaaaaaact gtccaaacgt cacggggcag tcagcgtaat gcagtatcgt gatgacggtt
     1021 atttgccaga agcactgctg aactatctgg tgcgtctggg ctggtcccac ggcgatcagg
     1081 aaatcttcac tcgtgaagag atgatcaaat acttcacttt gaatgccgtc agcaaatctg
     1141 ccagtgcgtt caacaccgac aagctgctgt ggctgaacca tcactacatt aacgcgctgc
     1201 cgccggagta tgttgctact cacttacagt ggcacattga gcaggaaaat atcgataccc
     1261 gtaacggccc gcagctggct gatctggtga aactgctggg cgaacgctgc aagacgctga
     1321 aagagatggc acagagctgc cgttatttct acgaagattt tgctgagttc gatgccgatg
     1381 cggcgaaaaa acatctgcgt ccggtagcgc gtcagccgct ggaagtggtt cgtgacaaac
     1441 tggccgcgat tactgactgg accgctgaaa acgttcatca cgctattcag gcgacggcgg
     1501 atgagctgga agtgggtatg ggtaaagttg gtatgccgct gcgtgtcgcc gtaaccggtg
     1561 cggggcagtc tccagcactg gatgttaccg ttcacgcaat tggtaagacc cgcagtatcg
     1621 agcgtatcaa caaagcgctg gattttattg ctgaacgcga aaatcagcag taattagcgt
     1681 gtaaaaagat aaacggcagg agataatatc ctgccgtttt ttatttatgc tgattcgtca
     1741 atatcgagac gggcgaggaa attgcgaatt ccttcacgtg acaaaaattg cgcgactttt
     1801 tcctgctcaa tgtctgtcat attatctatc gcgttgatga tttgtcgata cgccggggcg
     1861 cgtgctccag gagcgtattc agcaattctc tcttcagctt ccatcaacat tggtgtgtta
     1921 tggaaggctg gcgtgttctg aataaactcg caaacttgcg tatcgaccag aataagtctg
     1981 gcacgaccgc ctttcacgcc gggaaatttt tccgttttcc agcctttttc tctgatccaa
     2041 cggttaacag tttgtttagc cacaccgagg cattcagcca gttcttcggt ggtcattttg
     2101 ttgcgtaatc ttttcatatt atttgctatc gcgaatccct aagcgttgta acaatccgat
     2161 aatcccttcc cgcagaagca aggatgtaaa ctgtttttgt tcaactggcg tcatctcttt
     2221 cgccagtgtc accagcaata cttcaagcgg cgcatcacca gaaagggcag gcgcttctcc
     2281 ctgaccttct ggacgttcag cattgcgaat atattcacga acctgttcat tgacgtgaac
     2341 cagtcgggct ttgccaccct ggacgccagg ttttggtgac gttgtccagc cttccttgcg
     2401 tacccattta ttaatggtct ggcggctata gccggtcagt ctggcaagtt catctggtgt
     2461 catcctctcc ttgaacataa caattccctg aatgtatgtg ggtctattag tagttcattt
     2521 tataacacca ctttacagga aaccgtgaca tgaataacta ctgaatgcga gcggttttcc
     2581 aaaattgttc atttgcctga tttttcagcg attgaaacta cacgttgcaa tttgccgttg
     2641 acacattcgg gcggaattca tatgatgccg cccgtcaact cgacaagctt tacgtgacgg
     2701 ggctatagct cagctgggag agcgcttgca tggcatgcaa gaggtcagcg gttcgatccc
     2761 gcttagctcc accaaatttt gcacccagca aatttggtac gtaaacgcat cgtggggcta
     2821 tagctcagct gggagagcgc ttgcatggca tgcaagaggt cagcggttcg atcccgctta
     2881 gctccaccaa atttccaacc ctcgctgcaa agcgggggtt ttttgtctct gctttttgcc
     2941 gcttttgtaa tacagtctac gtccgggtta gcgccgcctg gtgaaagcat cattggatga
     3001 aaaatcggca acaggctggc ccctgtttgc ttcgcgatgc gaataaactt attatttgtg
     3061 tgcctgaaaa ccccgatcag tgagagtagt gtactcatgt ttgtggagca taacctgata
     3121 aaaaatatca agatattcac actagcgttt acgctcaccg tggtacttat tcagctatcc
     3181 cgttttattt cgccacttgc cattatccat tccagttata tctttctggc gtggatgcca
     3241 ctgtgcgtaa tgctgtcaat cttgtttatc tttggctggc gcggtgtcgt tcccgtttta
     3301 tgcgggatgt tttgcaccaa tctgtggaac tttcatctct cttttttaca gactgcggtc
     3361 atgctcggta gccagacgtt tgtcgtgttg tgtgcctgcg caatattacg ctggcagctg
     3421 gggacgcgtt ggcgttatgg attgaccagc cgatatgtct ggcaacgtct gttctggctt
     3481 ggtttggtga cgccgatcgg catcaaatgc agcatgtatc ttgtggggag cttctttgat
     3541 ttcccgctaa agatatccac ctttttcggc gatgcggatg ccattttcac ggtcgttgat
     3601 ttgctaagcc ttttcaccgc tgtgctgatt tacaacatgc ttttctacta tctcactcgc
     3661 atgattgtaa gtccccactt tgcgcagata ttgtggcgca gggatatcgc tccgtcgttg
     3721 ggcaaagaga aacgcgcatt taccttaagc tggctggcag cgttaagcgt gctgctactt
     3781 ctgttgtgca caccttatga aaacgacttt attgccggtt acttggtacc cgttttcttc
     3841 atcatcttta ccctcggggt cggtaagctt cgctatccgt ttttaaatct cacctgggct
     3901 gtttcaacgc tttgcctgct gaattacaac cagaactttt tgcaaggggt ggaaaccgaa
     3961 tattcgctgg catttattct tgcggtgctg atttccttta gcgtttgcct gctctatatg
     4021 gtgcgcattt atcatcgcag tgaatggctt aatcgccgct ggcatttgca ggcgctgaca
     4081 gatccgttaa cgctcctacc caactttcgt gcgttggaac aagcgccgga gcaagaggcg
     4141 ggcaagagtt tttgctgcct gcgcattgat aatcttgagt ttatgagtcg tcattacggc
     4201 ttaatgatgc gcgttcactg tatccgctca atttgccgta cgctgctgcc gttgatgcag
     4261 gaaaacgaaa agttgtatca attgccgggt agtgaactgc tgttagtgct gagcgggccg
     4321 gaaacggaag ggcgactcca gcatatggtt aacatcctga atagtcggca aattcactgg
     4381 aacaataccg ggctggatat gggctatggt gctgcctggg ggcgttttga tggaaatcag
     4441 gaaaccctgc aacccttgtt ggggcagtta agctggctgg cggagcaatc ctgcgcacat
     4501 catcatgtgc tggcgctgga tagcagagag gagatggttt ccgggcagac cactaaacag
     4561 gtgctattgc tgaataccat tcgcacggcg ttagatcagg gtgatttgct gctctacgcc
     4621 cagccaattc gcaacaaaga gggtgacggt tatgatgaga tcctcgcgcg actgaaatat
     4681 gacggcggca ttatgacccc ggataagttt ctgcccctta ttgctcagtt taaccttagc
     4741 gcgcgttttg atttgcaagt gctggaatcc ttgttgaagt ggctggcaac acacccttgc
     4801 gacaaaaaag gaccgcgctt ttcagtcaat ttaatgccgc tcacgctgct gcaaaagaat
     4861 attgccgggc ggattattcg tctgtttaag cgttatcaca tctccccgca ggcggtcatt
     4921 cttgagatca ccgaggagca ggcgttttct aacgcagaaa gcagcatgta caacatcgag
     4981 cagctgcata agtttggttt ccggattgcg attgatgact ttggcaccgg atatgccaac
     5041 tacgaacggt taaagcgttt gcaggctgat atcatcaaaa ttgatggcgt ctttgtgaaa
     5101 gatattgtca cgaatacgct ggatgcgatg attgtgagat caattaccga tctggcgaaa
     5161 gcgaagtcat tgagtgtggt cgcggagttt gtcgagacgc aacagcagca ggcgctattg
     5221 cataagctcg gggtgcaata tctgcaaggg tatttgattg gtcgcccgca gccattagct
     5281 gattaacagg cgtaaaaaaa ccggggaatt atccccggct tttttatgta tggtcttaca
     5341 gcaccagtgc tgctattgac gcagacagca cactcaccag ggtagagccg taaaccagct
     5401 tcagaccgaa gcgagaaacc acgttacctt gctcttcatt caggccttta actgcacctg
     5461 cgataatccc gattgaagag aagttagcga aggaaaccag gaacacagag atgatgcctt
     5521 cagcacgcgg agagagcgtg gaagcaattt tctgcagatc catcatcgca acgaactcgt
     5581 tggaaaccag tttggtcgcc atgatactgc ccacttgcag tgcttcactg gaaggaacac
     5641 ccatcaccca tgcaatcgga tagaagatgt agcccaggat gccctggaag gagatgctgt
     5701 agccaaacca gccagtaacg gtggcaaaca gtgcgttcag cgcggcgatc agggcgataa
     5761 agccgatcag catcgcggca acgataatgg caactttgaa acctgccaga atgtattcac
     5821 ccagcatttc gaagaagctc tgaccttcgt gcaggttgga catctggatg ttttcttcac
     5881 tggcatcaac acggtaagga ttgatcagcg acagcacgat aaaggtgctg aacatgttca
     5941 gtaccagcgc agcaacgacg tatttcggtt ccagcatggt catgtatgca ccaacgatgg
     6001 acatcgacac ggtggacatt gccgtggcag ccatggtgta catacgatta cgggagattt
     6061 tgccgaggat atctttatag gcaataaagt tttcagactg acccagaatc agggagctga
     6121 cggcgttaaa ggattccagt ttgcccatgc cgttgacttt ggagagcagg aaaccaattg
     6181 cgcggatgat caccggcaac acgcgaatgt gctggagaat accgatcagt gcagagataa
     6241 agacgattgg gcacagcact ttcaggaaga agaatgccag gccttgatca ttcatgctac
     6301 caaagacgaa gttagtccct tcgttggcaa atccgagcag tttttcgaac atttcggaga
     6361 agcctttcac gaagcctaaa ccaacgtcgg agttcaggaa gaaccacgcc agtaacactt
     6421 cgataacaag cagttgaata acataacgga tacgaatttt tttgcggtcg ctgcttacca
     6481 gcagtgcgag aatcgcaaca acggcaagtg ccagtacaaa atgaaggacg cggtccatat
     6541 ttgctccaaa tatgaggcag gttaaatttc cgtgcacatt ctatgtaaca tggttaagga
     6601 aaacgagatc tgacacacac tataatgacg tcctgtgaca agcttcaaaa atagtgatct
     6661 gtcatacatt tctgctatgt tgtgtatgga agctgaaagt tatgtaaatg tgctaacatt
     6721 aaacataacc ataacattca agtcatcaat cttttcgtag tcataatcct ggtctatcaa
     6781 agaaatcacc acaacccatt caaatgaatt tgataatcat tctcgtttgg catagcatga
     6841 aacatagcaa aggctatgtt ttagaggcac aagatgacga actatcgcgt tgagagtagc
     6901 agcggacggg cggcgcgcaa gatgaggctc gcattaatgg gacctgcgtt cattgcggcg
     6961 attggttata tcgatcccgg taactttgcg accaatattc aggcgggtgc tagcttcggc
     7021 tatcagctac tgtgggttgt cgtttgggcc aacctgatgg cgatgctgat tcagatcctc
     7081 tctgccaaac tagggattgc caccggtaaa aatctggcgg agcagattcg tgatcactat
     7141 ccgcgtcccg tagtgtggtt ctattgggtt caggcagaaa ttattgcgat ggcaaccgac
     7201 ctggcggaat ttattggtgc ggcgatcggt tttaaactca ttcttggtgt ttcgttgttg
     7261 cagggcgcgg tgctgacggg gatcgcgact ttcctgattt taatgctgca acgtcgcggg
     7321 caaaaaccgc tggagaaagt gattggcggg ttactgttgt ttgttgccgc ggcttacatt
     7381 gtcgagttga ttttctccca gcctaacctg gcgcagctgg gtaaaggaat ggtgatcccg
     7441 agtttaccta cttcggaggc ggtcttcctg gcagcaggcg tgttaggggc gacgattatg
     7501 ccgcatgtga tttatttgca ctcttcgctc actcagcatt tacatggcgg ttcgcgtcaa
     7561 caacgttatt ccgccaccaa atgggatgtg gctatcgcca tgacgattgc cggttttgtc
     7621 aatctggcga tgatggctac agctgcggcg gcgttccact tttctggtca tactggtgtt
     7681 gccgatcttg atgaggctta tctgacgctg caaccgttgt taagccatgc tgcggcaacg
     7741 gtctttggat taagcctggt tgctgccgga ctgtcctcaa cggtggtggg gacactggca
     7801 gggcaggtgg tgatgcaggg gttcattcgc ttccatatcc cgctgtgggt gcgtcgtaca
     7861 gtcaccatgc tgccgtcatt tattgtcatt ctgatgggat tagatccgac acggattctg
     7921 gttatgagtc aggtgctgtt aagttttggt atcgccctgg cgctggttcc actgctgatt
     7981 ttcaccagtg acagcaagtt gatgggcgat ctggtgaaca gcaaacgcgt aaaacagaca
     8041 ggctgggtga ttgtagtgct ggtcgtggcg ctaaatatct ggttgttggt ggggacggcg
     8101 ctgggattgt agttgaatga gcgtcgcatc tggcactatt ggcggatgcg gcgtaaacgc
     8161 cttatccgcc ctacgcgtta aagagccggt ttgtaggcct gataagacgc gctagcgtcg
     8221 catcaggcat tgtgctccat tggaggacgc gtttaatggc ggtgcccatg tccgcggcct
     8281 tttccgcgat gatcgtcacg atcgcgccag ccttcacgat agccacgctc atatgcttta
     8341 cgcttatccc agccacgatg atagccatta tcatgacgcc accagcggtt tttgcgccat
     8401 tcataattac gatgccagta atcacgatct cgccagtgac caccgtccca gtaattaccg
     8461 taatgatcgc gatcgccaat ttgtaatttg atcgatggca gtagggtgat ttcacctgca
     8521 tttgctgcaa gcggggtaaa tgccatcagg gcggccgcca gaaacagtga cctgaacatt
     8581 gttattctcc ttcacgatcg aagccgtcag cggcctgtta acgcaatatt acggggaggt
     8641 aaagccccgt atcatcgcca taactcttaa atcataaaga agagcatttt ttgctaaaca
     8701 ttatcgtggg gtggctgttg cttcggacga ttgaccatca tcatcggctt acctgctaat
     8761 aacagccatg ccggaagcat gacgatacag agcagcggta gcaatgtggt attgggtaca
     8821 acgactgccg ccataaaaag acttaaccag ccatcgcgtg ttaccaccag cacgatgccg
     8881 agaatagcgc aggaaacggt tattgccgct ggaacggcgg gaacgtgctc atgcagcatc
     8941 aaccctaatg ccacgccgac aaacactgcc gggaagattc gcccaccgcg aaagccactg
     9001 gcggcagcaa cgaccagggc ggcaagttta attaccgcca gcaaaaagta atcgctggtg
     9061 ctgaaagcct gatttgccac catctgctgc atctcatcca gccctttaaa cagcgaaacc
     9121 ggcccaccaa taactcccag aataccgaga ataaatccgc caatacctag cacgagcacc
     9181 ggatttttca tttgatgcat catcgcgtgc aaccgtggta agcaccatac ggcgaccatt
     9241 cctgctgcga tggcaatcgc cgcgacaatt gcaccgctga gaatatcggt catttccatc
     9301 tgtccgtaat gcgcaatggg cagtgaaaaa tggggatgga aaaataatcc ggtagtaagt
     9361 gcaccagctg ctgccgccat taatggcgca aaaagacgat cccatagcgg aacttcacta
     9421 ctgccattta aggtttgcga aaatatcaac gccgccgcaa caggtgtacc aaacagcgcg
     9481 ccgatggttc cggcagaggc taaaatagtc cactccattc ggttgacgcg cggtaacaga
     9541 cgagcgccaa tggcaaccgc aagggcgata ttgacggtca tgatcggatg ttccggcccc
     9601 aggctgacgc cgccagcaag accgagaatt aatgctacga taagtcctgg tagcgcagag
     9661 ggcggaactg gtgcgccgat cagcggttca caggcggggt ctggtccggc atgaccctgg
     9721 ctgaaacgga taaccaaccc caccgcaata cccgttagcg ttaatacacc gatgatccag
     9781 agaggtgaat cctgggctat ccccagagtt cccggcagtc gttgccagag caaattctgt
     9841 aataccgagg cgattttcat caccacaatc agaataagac tggacgcaat cccaattgcc
     9901 acggcgggga gcgataataa caacatggtt ctggctcgcg gatggagcat gttctattcc
     9961 ttatgcgggg tcagatactt agtttgccca gcttgcaaaa aggcatcgct gcaattggtg
    10021 ctgaaacgat aaagtaaatg tgtgacccag atcgatattt acagggagcc tgcctttccg
    10081 gcgttgttgt tatgccccca ggtatttaca gtgtgagaaa gaattatttt gactttagcg
    10141 gagcagttga agaatgacaa agtatgcatt agtcggtgat gtgggcggca ccaacgcacg
    10201 tcttgctctg tgtgatattg ccagtggtga aatctcgcag gctaagacct attcagggct
    10261 tgattacccc agcctcgaag cggtcattcg cgtttatctt gaagaacata aggtcgaggt
    10321 gaaagacggc tgtattgcca tcgcttgccc aattaccggt gactgggtgg caatgaccaa
    10381 ccatacctgg gcgttctcaa ttgccgaaat gaaaaagaat ctcggtttta gccatctgga
    10441 aattattaac gattttaccg ctgtatcgat ggcgatcccg atgctgaaaa aagagcatct
    10501 gattcagttt ggtggcgcag aaccggtcga aggtaaacct attgcggttt acggtgccgg
    10561 aacagggctt ggggttgcgc atctggtcca tgtcgataag cgttgggtaa gcttgccggg
    10621 cgaaggcggt cacgttgatt ttgcgccgaa tagtgaagaa gaggccatta tcctcgaaat
    10681 actgcgtgcg gaaattggcc atgtttcggc ggaacgcgtg ctttctggcc ctgggctggt
    10741 gaatttgtat cgcgcaattg tgaaagctga caaccgcctg ccagaaaatc tcaagccaaa
    10801 agatattacc gaacgtgcgc tggctgacag ctgcaccgat tgccgccgcg cgttgtcgct
    10861 gttttgcgtc attatgggcc gttttggcgg caatctggcg ctcaatctcg ggacatttgg
    10921 cggcgtgttt attgcgggcg gtatcgtgcc gcgcttcctt gagttcttca aagcctctgg
    10981 tttccgtgcc gcatttgaag ataaagggcg ctttaaagaa tatgtctatg atattccggt
    11041 gtatctcatc gtccatgaca atccgggtct tctcggttcc ggtgcacatt tacgccagac
    11101 cttaggtcac attctgtaaa tccttcctgt tatatcggga ggtaactctc ccgataatct
    11161 tttaaatcat acagttaatt caatttttct ttgtgtcccc tcacaagacc gacctgcgtc
    11221 acacttccgt acagcgggat taattctcca gtaaatgcat tatttgtctg gtaacggcga
    11281 tttgttttgc acgttcataa tttcactcgt caactatcac tgaatgagga attaacgatg
    11341 agtaagaaac taattgcctt atgtgcctgc ccaatgggcc tggctcacac ttttatggcc
    11401 gctcaggcgc tggaagaagc ggcggtagaa gccggttatg aagtgaaaat tgaaacccag
    11461 ggcgcggacg gtatccagaa tcgcctgacg gcgcaggata tcgccgaagc aaccatcatc
    11521 atccactccg tggcagttac cccggaagat aatgagcgtt tcgaatcacg cgacgtttat
    11581 gaaatcactt tgcaggacgc aattaaaaac gctgcgggca tcatcaaaga aatcgaagag
    11641 atgattgcct ctgaacaaca gtaatattcg acagggataa ttgttatggc cattaaaaaa
    11701 cgcagtgcaa ccgttgtgcc tggcgcatcc ggtgcggcag cggcagttaa gaatccgcag
    11761 gcctctaaaa gcagcttctg gggtgaactc ccgcagcatg tgatgtcagg gatttcacgc
    11821 atggtgccga ccttaattat gggcggtgtg atcctcgctt tcagccagct gattgcttat
    11881 agctggctta aaattcctgc tgagatcggc atcatggatg ctcttaatag cgggaaattc
    11941 tccggtttcg acctctcctt actgaaattt gcctggctgt cgcagtcctt cggcggcgtg
    12001 ttgtttggtt ttgccattcc gatgtttgct gcttttgtgg cgaactctat cggtggcaaa
    12061 ctggcgttcc cggctggttt tatcggcggc ttgatgtcta cccagccgac gcaactgctg
    12121 aacttcgatc ccagcactat gcaatgggcg acctcttcgc cggtgccgtc caccttcatt
    12181 ggcgcgctga ttatttctat cgttgccggt taccttgtga aatggatgaa ccagaaaatc
    12241 cagttaccgg atttcctgtt agcgttcaaa accacatttt tgctaccgat tctttccgcc
    12301 atttttgtca tgctggcgat gtactacgtc atcaccccgt ttggcggctg gatcaacggc
    12361 ggtatccgta ctgtgctgac tgccgcaggt gagaagggcg cgctgatgta cgcaatgggg
    12421 atcgccgcgg cgacagcaat cgaccttggt ggcccgatca acaaagccgc aggtttcgtt
    12481 gccttcagct ttaccactga ccacgtatta ccggtcaccg ctcgttctat cgctatcgtt
    12541 attcctccga ttggtctggg tctggcgacc attattgacc gtcgtttaac cggcaaaggc
    12601 ctgttcaacg ctcaacttta tccgcagggt aagaccgcga tgttccttgc ctttatgggg
    12661 attagtgagg gtgcgattcc gtttgcgctg gaaagcccca tcaccgccat tccgtcgtat
    12721 atggtcggcg cgattgtcgg ctcaaccgcc gctgtctggc tgggcgcagt gcaatggttc
    12781 ccggaatccg ctatctgggc atggccgctg gtcactaacc tgggcgtcta tatggcgggg
    12841 atcgcgctgg gggcagtcat aactgcgctg atggtggtgt tcctgcgtct gatgatgttc
    12901 cgtaaaggca aattgttaat cgatagcctg taagaaggaa aatccagatg acattactcg
    12961 cttcgctgcg cgactggctt aaggcgcaac aactggatgc agtgcttctc tcctcacggc
    13021 agaacaaaca gccgcatctg gggatctcca ccggatcagg ctatgtggtt attagccgtg
    13081 aaagtgcgca cattctggta gattcgcgct atttcgttga ggtggaagcc cgtgcgcaag
    13141 gctaccagct gcatttgctt gacgcgacga acacgcttac cactatcgtc aatcaaatca
    13201 ttgccgatga gcagttgcaa acgctcggtt ttgaaggcca gcaggtgagt tgggaaaccg
    13261 cgcaccgctg gaagtctgaa ctcaatgcga aactggttag cgccacgccg gatgtgctgc
    13321 ggcaaatcaa aacgccagag gaggtggaga taatccgcct cgcctgtggg attgccgatc
    13381 gcggagcgga gcatattcgc cgctttattc aggcggggat gagcgagcgc gagatagccg
    13441 ctgaactgga gtggtttatg cgccagcagg gcgcagaaaa agcctctttt gacaccattg
    13501 tcgccagcgg ctggcgcggg gcgctgccgc acggcaaagc cagcgacaag attgttgcag
    13561 cgggcgagtt tgtcactctc gattttggtg cgctgtatca gggctactgc tctgatatga
    13621 cgcgcacctt gctggtgaat ggcgaagggg tgagcgccga atctcacccg ctgtttaacg
    13681 tctaccagat tgtcctgcag gcacagctcg cagcaatctc tgcaattcgc cccggcgtgc
    13741 gctgccagca ggttgacgat gccgcgcgcc aagtgattac cgaggcaggt tttagccact
    13801 atttcggtca taacaccggt catgctatcg gcattgaagt tcatgaagat ccgcgttttt
    13861 caccgcggga caccacgacg ctacagccag gcatgttact gaccgtggag ccggggattt
    13921 atttgccagg gcaagggggc gtgcgcatcg aagatgttgt gctggttacc ccgcaaggcg
    13981 cagaagtgct ctacgccatg ccgaaaacag tgttgctcac gggagaggca taatggattt
    14041 atcgctatta aaagcgttga gcgaggcaga tgcgatcgcc tcctcggaac aggaagtgcg
    14101 gcagatcctg ctggaagaag cggatcgcct gcaaaaagaa gtgcgatttg atggtctggg
    14161 atcggtgctg atccgcctca atgaatcgac aggtccgaag gtgatgatct gcgcccatat
    14221 ggacgaagtg ggatttatgg tgcgtagcat ctcccgcgaa ggggcaattg atgtgctgcc
    14281 ggttggcaac gtgcgcatgg ctgcccgcca gctgcagccg gtgcgcatca ccacccgtga
    14341 agagtgcaaa attccaggcc tgcttgacgg cgaccggcag gggaatgacg tcagcgccat
    14401 gcgcgtggac attggtgcgc gctcgtatga cgaagtgatg caggcgggaa ttcgtccagg
    14461 cgatcgcgtc acctttgata caacttttca ggttctccct caccagcggg tgatggggaa
    14521 agcctttgat gatcgcctcg gttgctatct gctggtgacg ttactacgtg agctgcacga
    14581 tgccgaactg cctgcggagg tgtggctggt ggcaagttcc agcgaagagg tgggattacg
    14641 cggtgggcaa accgctaccc gcgcggtgtc gccggacgtc gccattgtgc tggataccgc
    14701 ctgctgggcg aaaaactttg attatggcgc ggctaaccat cgccagattg gtaacggccc
    14761 gatgctggtg ttaagcgaca agtcactgat tgcgccgcca aaacttaccg cctggatcga
    14821 aaccgtggcg gctgaaattg gcgtaccgtt gcaggcagat atgttcagca acggcggcac
    14881 ggacggcgga gcggtgcact taaccggtac tggcgtaccc acagtggtaa tggggcctgc
    14941 cacccgccac ggacattgcg cggcatcgat tgccgattgc cgcgacattt tgcagatgga
    15001 gcaactttta tctgccctta ttcaacgtct tacgcgtgag acggttgttc aactgacgga
    15061 tttcagatga tctcctgatt aacccggagc ggttatgtta acgattcaat ttctctgtcc
    15121 tttgcctaac ggtctacatg ctcgtccggc gtgggaactt aaagaacagt gcagccagtg
    15181 gcaaagcgaa atcacctttt tcaaccatcg ccagaacgca aaggccgatg ctaaaagctc
    15241 gctggcgttg attggaactg gcaccttatt taatgacagt tgcagcctga acattagcgg
    15301 cagcgatgaa gagcaggcgc ggcgcgtgct ggaagagtac atcctggtgc gctttatcga
    15361 cagcgacagc gttcagccga cgcaggcaga actgacggcg catccgctgc cgcgttcatt
    15421 aagccgcctt aacccggatt tactgtacgg caatgtgctg gcaagcggcg tcggcgtggg
    15481 tacgctgacc ctgttacaaa gcgacagcct cgacagttat cgggcaatcc ccgccagtgc
    15541 gcaagattcc acccggctgg agcacagcct ggcaacgctt gccgagcaac tgaaccagca
    15601 attgcgtgag cgtgacggcg aaagcaaaac tatcctcagc gcccatttgt cgctgattca
    15661 ggatgatgaa ttcgcgggca atatccgtcg tctgatgaca gaacagcatc aagggctggg
    15721 ggcggcgatc atcagcaata tggagcaggt ttgcgccaaa ctttctgctt ctgccagtga
    15781 ttatctgcgc gaacgtgtta gcgacattcg cgatattagc gaacagttgc tgcatatcac
    15841 ctggccggaa ctgaagccgc gcaacaatct ggtgctggaa aaaccgacta ttctggtagc
    15901 cgaggattta accccaagcc agtttttgag cctcgattta aaaaatcttg caggcatgat
    15961 tctggagaaa actgggcgca cctcgcatac cctgattctg acccgcgcct cggcgatccc
    16021 ggtactgagc ggcctgccgc tggatgcgat tgcccgttat gccgggcaac cggcggtgct
    16081 tgacgcccag tgcggcgtgc tggcgattaa cccgaatgac gcggtgagcg gttattatca
    16141 ggtcgcacag acgctggcgg ataaacgcca aaaacaacag gcgcaggccg ccgcgcagct
    16201 ggcctattcc cgtgataaca agcgtattga tattgcggcg aatatcggca ccgctctgga
    16261 agcgccaggc gcgtttgcca acggcgcgga aggcgttgga ctgttccgta ctgaaatgct
    16321 ctatatggat cgcgacagcg cgccggacga gcaggagcaa tttgaagcct accagcaggt
    16381 gctactggca gcgggcgaca agccgattat cttccgcaca atggacatcg gtggcgataa
    16441 aagcattcct tatctgaata ttccccagga agagaacccg ttcctcggct atcgcgcggt
    16501 acgtatttac ccggaatttg ctggcctgtt ccgcactcaa ctacgggcga ttttgcgcgc
    16561 cgccagtttt ggcaacgccc agttgatgat cccgatggtc cacagtctcg atcagatctt
    16621 atgggtgaag ggcgagatcc aaaaagcgat cgttgagctt aagcgcgatg gcctgcgtca
    16681 tgcagagacg attacgctgg ggatcatggt ggaagtgccg tcggtgtgct acatcatcga
    16741 tcacttctgc gatgaggtcg atttcttcag tatcggctcc aacgatatga cccagtatct
    16801 gtatgcggtc gatcgtaata acccgcgcgt atcgccgctg tataacccga ttacgccatc
    16861 gttcctgcgc atgttgcagc agattgttac cactgcgcat cagcggggca aatgggtagg
    16921 catttgcggt gaactgggcg gtgaaagccg ttatctgccg ctactgcttg ggctgggcct
    16981 ggatgagctg agtatgagta gcccgcgtat tccggcggtg aaaagccagc ttcgtcaact
    17041 ggatagcgag gcgtgtcggg aactggcgcg tcaggcatgt gaatgccgca gtgcgcagga
    17101 aattgaagcg ttactcaccg cctttacgcc ggaagaagac gttcgcccac tgctggcgct
    17161 ggagaatatc tttgttgatc aggattttag caataaagag caggcgatcc agttcctgtg
    17221 cggcaacctc ggcgttaacg ggcgcactga acatccgttt gagctggaag aagatgtctg
    17281 gcagcgggaa gagattgtca ccaccggcgt tggttttggc gtagcgatcc cgcacaccaa
    17341 atctcagtgg atccgtcatt ccagtatcag cattgcccgg ctggcgaaac cggttgactg
    17401 gcagtcagaa atgggcgaag tcgaactggt gatcatgctg acgctgggtg ctaacgaagg
    17461 gatgaatcat gtgaaagtct tctcgcagct ggcgcgtaaa ctggtgaata aaaacttccg
    17521 ccagtcgttg tttgccgcgc aagatgcaca aagtatcctg acgctgctgg aaacagaatt
    17581 aaccttctga cgttagccct gaaaacgggc gctgtactct cccggcgtca gaccaaactg
    17641 acgccggaaa acgcgacaaa aatagtcgct atccggaaaa ccgcaacgct gtgccacttc
    17701 atgaattgac agatgatatt tctgcaaaat catcctcgcc ttcgccattc gcacccaacg
    17761 cacatactcg ataaaacgca tcgttccatg ctgagcaaac agttttgaca gatgattggg
    17821 cgtgatatta aaaaactgcg ccacgctctc gcgggtgagc ggctgggcat aattatcctg
    17881 aacccagtta caaatgctgt gatagagaaa atcgccgcgc ggttgcgaat tacccggcgg
    17941 catattcacc acgttgcggc aaagatgcag caaacttaac accagtggct gaatgatatt
    18001 ctgctccagc ggcgaacggc taagatgggt gagggcggta agcatcgctt caccttcacc
    18061 gcgttgcagg ctgggaagct ggatctggcg agcaggatgc aacaacgacg tggtgcggct
    18121 atcgtaaaac gataacccaa gccaggtcgg tgcgaacacc aggcttaaca gcatcaccgg
    18181 tttgttgttg atcggtaaat tagccgcgcg tgccggaata aacagcattt ctccttccac
    18241 cattcgctgc tgctttgctt ccagcaggtt gccgtattcg ccgcgcagca cgatatccag
    18301 acggggaaaa tcaatacata aactgcccac cggtaacgag gcaggctggc tggcaaacca
    18361 gacgcgcccc agtagttgcg ggttaagcac caggccgctg aacagatcgg cgaaaaattg
    18421 ttgatcggcg ggcaagcctg gtgctttcat gaaagttccc gattaaagat gcattaactg
    18481 gcgaaattct ttcactttgc tgcgactgac cggcacttca aaatccagat ctttcagtcg
    18541 cagaatgtag gtgttattaa accacggttc gatttcgcgt attttgttca gattgacaca
    18601 aaatgagcga tggcagcgga aaaaatgcga cggcggcagt ttgctgcaaa attcggtaat
    18661 gttcatcggc attacgtagg attcacgccg cgtatagaca aaggtcattt tctcgtgcgc
    18721 ttcggcgtaa tagatatcgt taattggcgt gacgattatt cgctcatctt tcaccagatt
    18781 aatggtgtca ttctcacgcg ttaccgtcgc ggcaggcgta ctgctggtct gctgttgttg
    18841 ccaggccgct tccagttttt gcagcatccc ggtaatacgt gactcctgat acggtttgag
    18901 aatgtagtca aacgcctcca gttcaaacgc ttctaccgca tgttctttcc acgcggtgat
    18961 gaacacaata aacggtttat tggcgaactg gctgatgttt tgcgccagca acacgccatc
    19021 cagcgacgga atattgatat ccagaaaaat ggcgtcgacg cggttatgct gcaaaaactt
    19081 caacacgtcc agaccgtcgt caaaggtgcc gacaatctcc atctggctgt gctctttaat
    19141 tagccagctc agttcctgtt gtgccaggaa ttcgtcttca acaatgatga ctttcacaat
    19201 atcactccgg ctcaaagcaa taacgtagcc tgtgaggcga ctggggtgcg ttggttagga
    19261 atgtaaaacg caatttccgt ccccggctcc aggcggcgga tatgcagccc ctcgccatac
    19321 aataacttca cgcgatgatg gacattcagc aggccaattt tattgcccgg catttcattc
    19381 gcttcgaccc gctcaatcac ctttggatcg atgccgtgcc cggtatctcg caccgcaatg
    19441 cgtacccgat ttccgcactc tgcaacgctg atggtgacaa cgcctttacc tttgcaaggc
    19501 tgaataccgt ggacaatggc gttctccacc aacggctgga tcagcaggct gggaatgcag
    19561 caattcacct cttcatcaat atcatagata accgtcagct tgtcaccaaa gcgggcctgc
    19621 tcaatggcaa tatagtcttt aatttgatac agctcttttt tgatatcgat ttgctcatcg
    19681 tcttttaatt caatgttata gcgcagataa cgcgacagat taaagatcaa ctggcgagcg
    19741 gtatccggat tcagacggat tgacgatgaa atagcgttca gagcgttaaa cagaaaatgg
    19801 ggattaattt tgctttgcag ggcgcgaagc tctgccttat ttgccatttc acgtagctgc
    19861 tcggcgcgtg aaacctccag ttgcgtggag atgatttgcg acagaccgac cgccatttcc
    19921 tgtaatgacg aggtgatctg atgcgcgtgg cagtagtaaa ttttcagcgt tccggtcacg
    19981 acccctttct cccacaatgg gatcaccagc atggaatgaa tctctggtgt gcggtgggct
    20041 tcatcattgt ttttaatgat gatttttccg taattcatcg cctgacgggt agtcgggcta
    20101 atgaagtcat cgccattctg atagttatgt tcacccacgc caacataggc cagcacatga
    20161 tcggtattag taatcgccac cgcatcggcg tgaatgtcat cgcggataat ttcgcagacc
    20221 ttgcgtaatg actcgctatt gacatggcga aacagcggta gcgttttgtt ggcgatatcc
    20281 agcgccagct tggcctgccg cgcggcgctg gcctcttttt cgccctcaac gctttgcacc
    20341 agaagcacaa taaagccgat acagacgcta ccgaggatca ttggaatgcc gattttagag
    20401 acgatatcga tccccaacgc ggtagttggt gcccagacaa ttaccagaat catggtcaac
    20461 gtctcacaca acatgccgcc gagaataccg acgcgccagc gctgtgcttt ggggattttc
    20521 aggttgatcc agcccgatat acaacccgcc agaatgctgg tgataaagca ggggatcgcc
    20581 gtcacgccgc cgatatcaat taaataccgg tgaatacccg caatcacgcc ggtaatgatg
    20641 cctacccacg ggccaaacag aatcccgccg gacatcaccg cgataatacg tacgttcacc
    20701 agcgagcctt ctacgggaac gccggaccag gtgctgaaca gggcgaacag cgagaaaatg
    20761 gcggtaacgg cgagcaattc ttttggggag tgcgccgact tgtgcaacag ttcgcgaaac
    20821 aggcggatac ggatgagaaa gaacaggcag ataagcatta acgctgcccg atcgaagacc
    20881 gccagcagca tgttgaatat ttcgtgcacg ggtgactctg aataaggatt aaacctctat
    20941 gataggtaac ctgaaggctg atgaccagca ggccgttttt gaggaaaaac cataaattta
    21001 aaaggggatt gaaagataaa tggtgtatta gcaatgtatc tcttgataat gcacttctgg
    21061 ttggtaaatt tattgaaatt ataatgttaa taaaattggt ggtaattttc ttgttagcgt
    21121 agaaaaattt cctgtttttg aagtggagtt cctcatttaa agccattgat aaaaatgttc
    21181 taaaacgctg gacagacaga aattaatcag gctattttca aaacagccac cattgtggca
    21241 gcgtcgctcg gacggtccgg gcgctaacgt taatctgagg aaattatggc tgacactcgc
    21301 cctgaacgtc gctttacgcg cattgatcgt ctcccgccct acgtttttaa catcaccgct
    21361 gaactgaaaa tggctgcgcg tcggcgcggc gaagatatta tcgatttcag catgggtaac
    21421 ccggacggtg cgactccgcc gcatatcgtc gaaaaattat gtactgtggc ccagcgcccg
    21481 gacacgcatg gttactccac ttcacgcggc attccgcggt tacgtcgcgc catttcccgc
    21541 tggtatcagg atcgctacga cgttgaaatc gacccggaat cagaagccat cgtcactatt
    21601 ggttcgaaag agggcctggc gcatctgatg ctggcgacgc tggatcatgg tgacacggtg
    21661 ctggtgccga atccaagtta cccgatccat atttatggcg cggtgattgc cggggcgcag
    21721 gtacgctcag tgccgctggt ggaaggtgtc gatttcttca acgaactgga gcgcgccatt
    21781 cgtgaaagtt atccgaaacc gaagatgatg atcctcggct tcccgtcgaa tccaaccgcg
    21841 caatgcgtgg agctggagtt cttcgaaaag gttgtggcgc tggcgaaacg ctacgatgtg
    21901 ctggtggtcc acgacctggc ctatgccgat atcgtctacg atggctggaa agcgccgtca
    21961 atcatgcagg taccgggtgc acgcgatgtg gcagtcgagt tctttacgct gtcgaaaagc
    22021 tacaacatgg cgggctggcg tatcggcttt atggttggca acaaaacgct ggtcagcgcc
    22081 ctggctcgta ttaaaagcta tcacgattac ggcaccttta cgccgttgca ggtggcggcg
    22141 attgcggcgc tggagggcga tcaacagtgc gtgcgcgaca ttgccgaaca gtacaaacgc
    22201 cgccgtgatg tattggttaa agggctgcat gaagcgggct ggatggttga aatgccgaag
    22261 gcttcgatgt atgtctgggc gaaaatcccg gaaccatatg cggccatggg atcgctggaa
    22321 tttgccaaga agctccttaa cgaagcgaag gtttgtgtct cgccggggat tggctttggc
    22381 gactatggtg atacgcatgt tcgctttgca ctgattgaaa accgcgaccg tattcgtcag
    22441 gcgattcgtg ggattaaagc gatgttccgt gccgacggtt tactacccgc cagcagcaaa
    22501 catattcacg aaaacgcgga ataaacaccg tcgtcagaaa tgcaaacagg agcctgatgg
    22561 ctcctgtttt tcattgcaca gtttgctttt agatcatcag ggcaaaagta ccggcccaaa
    22621 cgatgaccat aaaagaaatg cccataaaga aatatttcac tttacatctc tccgcgtaat
    22681 tactgttacg cctgaatgac ttcattacac ctgatacagt gagttgacac cgggcaaagc
    22741 gaccttaaaa gccctgtatt ttaccgtggg catcacccca gaattctgag ctaaactgct
    22801 cgagacggcg ctaatgtacg cctaaaactg aaacgagaca agagacattt tcttatttac
    22861 ttttagtaac ttacaagtgt ctcatatcgg cgacagttta atttctgacg gtaataatta
    22921 gatattcagc gaaggaaaaa accttaagaa aaaagccata aaaaccatga ggttattatg
    22981 gccgatttga ggagggaaag agtaagagca gtttgttaaa tgtacaacga cgattctccc
    23041 accgggcgcg ttttaaagcg acggtggatc cagaggtact gctccggtgc gcgcatgatc
    23101 tctttctcga taatcttgtt catataggca gcggcttgat tttcatctgt cgggtagcct
    23161 tccatctctg gggtgatgaa caaacgatat ccgctgtaat ccgcttttct taccatcgtt
    23221 acggtcaaca tggctgcgcc agagagacgg gagagaacat aggtgccatt ggttgtggcg
    23281 acattttcca ccgcaaagaa cggcgcgaag gagctgcctt tacgaccata atcctgatcg
    23341 ggagcaaacc ataccgcttc acctttcttc agtgcaccga caatgccgcg cagattattt
    23401 ctgccgatca tcgctttgtt agagcgcatg cgcccacggg tctgcaccca ttccatcagc
    23461 tgattattat gtggacgata ggtagccatc attggttggc acagtcccat cacgcggccg
    23521 cccagttcca gcgacataaa atggacgccg acaaccatta cgccgcgatt ttgcatttgt
    23581 gcgcgtttaa ggttatccaa cccttcaaca tcaaaccatt tacgtacgcg actgtcgggc
    23641 cagaaccatg ccatgccggt ttctaccagc gccatgccga gtgaacgaaa gttttcggca
    23701 atcatcttct cgcgttcttc cgcagaatgc tgcgggaaac aaagttccag gtttttacgg
    23761 gcgatagatt cacgacgttt caggaatggt cgcgccattg cgccaatacg cgtgccgaga
    23821 aagcagagaa caggataagg aagctgtacc caaagccaga gtacaccaag cccaaaccat
    23881 gtgagccagt agcgaggatg tagaaactcg cgggaaaatt tgcattgtgg aaacatagtt
    23941 accttctgta gatacgaatt gttgtatcgc agaagattcc atgtcgacag cggtacactc
    24001 aattggttgg gccatcatgt acgacaatgg ttcctgtaaa gagtgtaaag actagtcagg
    24061 acataaccac gaggagcatg aatgcggtat ctgtacttta tcactcgctg actgctttca
    24121 gctactgatt attaatcgtg caaagtaaat atttttccgt acacaacagt cttgcattta
    24181 acatgctgtt ttcattgatg cttagcaaaa attgccagag gttaaggatt gtcttactat
    24241 taaagtgact gaagttacag gttttttagc gcggcaggag gttgagctat gatttattta
    24301 tggatgtttc tcgccctgtg tattgtctgc gtaagcggct acatcggtca ggtactgaac
    24361 gtggtatcgg cggtctcttc tttcttcggc atggtgatcc tcgctgcact catttattac
    24421 ttcaccatgt ggttaactgg cggtaatgaa ctagtgacgg ggatatttat gtttcttgcc
    24481 ccggcttgtg gcttgatgat tcgctttatg gtggggtatg gcaggcgata gtgggtcagg
    24541 atggctcatt tgggcaggtg ttcacatcag catcagacat gattgttcac ctgccatgtg
    24601 acagacgcta tcgcacagga tgaataagat aattcgcctt tgtcggataa gtaggcgtga
    24661 tttcttctgt gaggtttaat ggtggcgcat cttctctcat cttaaacaaa atcgcctgtg
    24721 atggctgaag ttttgtagag tgtgtggttg tcatcgctga tgttttttca gtctctttat
    24781 agctcctctc acttacacca ccagaaatgg aaaatgcaaa aacattgcat gccataatag
    24841 agaccaataa aaataagctg aaaagtatta agtttacttt catagaaagt tatcctgaaa
    24901 atggttcact gagaacctac gatgttatgg ctgtatttac atataatta
//
LOCUS       DAZGLJ010000049        24876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000049 DAZGLJ010000000
VERSION     DAZGLJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.049"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..1013
                     /gene="yqiI"
                     /locus_tag="ACHK49_001797"
                     /pseudo
     CDS             1..1013
                     /gene="yqiI"
                     /locus_tag="ACHK49_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744394.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(1056..1256)
                     /gene="glgS"
                     /locus_tag="ACHK49_001798"
     CDS             complement(1056..1256)
                     /gene="glgS"
                     /locus_tag="ACHK49_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO9262458.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            1525..2154
                     /gene="yqiJ"
                     /locus_tag="ACHK49_001799"
     CDS             1525..2154
                     /gene="yqiJ"
                     /locus_tag="ACHK49_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO9262459.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            2181..3842
                     /gene="yqiK"
                     /locus_tag="ACHK49_001800"
                     /pseudo
     CDS             2181..3842
                     /gene="yqiK"
                     /locus_tag="ACHK49_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
     gene            4081..4140
                     /locus_tag="ACHK49_001801"
     CDS             4081..4140
                     /locus_tag="ACHK49_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO9262460.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            4457..4516
                     /gene="ibsE"
                     /locus_tag="ACHK49_001802"
     CDS             4457..4516
                     /gene="ibsE"
                     /locus_tag="ACHK49_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO9262461.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(4636..6069)
                     /gene="hldE"
                     /locus_tag="ACHK49_001803"
     CDS             complement(4636..6069)
                     /gene="hldE"
                     /locus_tag="ACHK49_001803"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO9262462.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(6117..8957)
                     /gene="glnE"
                     /locus_tag="ACHK49_001804"
     CDS             complement(6117..8957)
                     /gene="glnE"
                     /locus_tag="ACHK49_001804"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO9262463.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIVHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(8980..10281)
                     /gene="ygiF"
                     /locus_tag="ACHK49_001805"
     CDS             complement(8980..10281)
                     /gene="ygiF"
                     /locus_tag="ACHK49_001805"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO9262464.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYHDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            10523..11143
                     /gene="ygiM"
                     /locus_tag="ACHK49_001806"
     CDS             10523..11143
                     /gene="ygiM"
                     /locus_tag="ACHK49_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO9262465.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            11207..12445
                     /gene="cca"
                     /locus_tag="ACHK49_001807"
     CDS             11207..12445
                     /gene="cca"
                     /locus_tag="ACHK49_001807"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO9262466.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPLTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(12608..13429)
                     /gene="bacA"
                     /locus_tag="ACHK49_001808"
     CDS             complement(12608..13429)
                     /gene="bacA"
                     /locus_tag="ACHK49_001808"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO9262467.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(13519..13887)
                     /gene="folB"
                     /locus_tag="ACHK49_001809"
     CDS             complement(13519..13887)
                     /gene="folB"
                     /locus_tag="ACHK49_001809"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO9262468.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            13992..14609
                     /gene="plsY"
                     /locus_tag="ACHK49_001810"
     CDS             13992..14609
                     /gene="plsY"
                     /locus_tag="ACHK49_001810"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO9262469.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(14622..15554)
                     /gene="ttdR"
                     /locus_tag="ACHK49_001811"
                     /gene_synonym="dan"
     CDS             complement(14622..15554)
                     /gene="ttdR"
                     /locus_tag="ACHK49_001811"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO9262470.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            15761..16672
                     /gene="ttdA"
                     /locus_tag="ACHK49_001812"
     CDS             15761..16672
                     /gene="ttdA"
                     /locus_tag="ACHK49_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO9262471.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            16669..17274
                     /gene="ttdB"
                     /locus_tag="ACHK49_001813"
     CDS             16669..17274
                     /gene="ttdB"
                     /locus_tag="ACHK49_001813"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO9262472.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            17322..18785
                     /gene="ttdT"
                     /locus_tag="ACHK49_001814"
     CDS             17322..18785
                     /gene="ttdT"
                     /locus_tag="ACHK49_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO9262473.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(18828..19841)
                     /gene="tsaD"
                     /locus_tag="ACHK49_001815"
     CDS             complement(18828..19841)
                     /gene="tsaD"
                     /locus_tag="ACHK49_001815"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO9262474.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            20079..20294
                     /gene="rpsU"
                     /locus_tag="ACHK49_001816"
     CDS             20079..20294
                     /gene="rpsU"
                     /locus_tag="ACHK49_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO9262475.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            20405..22150
                     /gene="dnaG"
                     /locus_tag="ACHK49_001817"
     CDS             20405..22150
                     /gene="dnaG"
                     /locus_tag="ACHK49_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO9262476.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNLELAKK"
     gene            22345..24186
                     /gene="rpoD"
                     /locus_tag="ACHK49_001818"
     CDS             22345..24186
                     /gene="rpoD"
                     /locus_tag="ACHK49_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO9262477.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(24265..24771)
                     /gene="mug"
                     /locus_tag="ACHK49_001819"
     CDS             complement(24265..24771)
                     /gene="mug"
                     /locus_tag="ACHK49_001819"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO9262478.1"
                     /translation="MVEDILALGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT         6115 a         6695 c         6259 g         5807 t
ORIGIN      
        1 tgtatgggca atggactgct atgctgaaca tgaaggggga aataccgtag tcattggtta
       61 cgtaccaaga atttctatcc ccagtaatgg taaaaaaggt gataagatct ggcaaagcag
      121 tgagtatttt atgaatgtct tctgtaataa tgcattacct gccccatcac ctggagaaga
      181 atacccatca gcctggacta atataatgat gtttttacca ggcggtcagg acttttataa
      241 tcagaactcc tatatttttg gcgtaacata caatggagtt gattatgatt cgactgcccc
      301 taatgctatc gcagcaccag aatgcattga tataaaagga gcgggtacct ttaataatca
      361 ctataaaaaa ccccgctgtc tgtagtggag gtcctgagcc tcaattatca gtaacctttc
      421 cagctcgcgt acaactctat attaaattag caaaaaatgc caatagagtt aataaaaacc
      481 tggtgttacc tgatgaatat atcgctctag agtttaaagg tatgagtggc gcaggagcta
      541 tagaagtaga caaaaatttg accttcagga ttcgtggatt aaataatatt catgtccttg
      601 actgctttgt taatgtcgca ctggaaccag ctgatggcgt tgttgacttt ggtaaaataa
      661 attcccgaac aattaaaaat accagcgtta gtgagacgtt tagtgtagtc atgacaaaag
      721 atcctggtgc ggcctgtact gagcaattca atattttagg gagctttttc actacggata
      781 ttttgagtga ttatagccat ctggatatgg gtaatggact gctattgaag atatttcata
      841 aagatggaac agcaacggaa tttaaccgct tctcacaatt tgcttctttt tcatcgtcta
      901 gtgcaccttc ggtcaccgca ccattcaggg cagaacttag tgcgaatccg gcagagactg
      961 ttgttgaggg accgtttagt aaagacgtca tcctcaaaat cacctataac tagcatctaa
     1021 tacaaacact aaaacgggcc atcaggcccg ttatatcagt gctctaactc cagctctctt
     1081 gcctgcatca tctgttgaca ataccaggca taacgtgcgc aaaaccaggt tctatgttct
     1141 tcatccatgt taccagtaac ggcaagaata tcgaccgggc gaccttctgc gtgcattctg
     1201 gcaaaactac gcgccaggaa atcgaaatta tttaaagaat taagactatg atccataagc
     1261 acatcctccg gttatcgcat accttttaag gccatatgct ttttctgatt tcagtattcc
     1321 ccaatcagtg tagtgccttt aaacataacg tgcgtaaata tattgtcccc gatcacactt
     1381 tttagtgaaa aataaaaaac catattaaaa aactttctct tccagaactt ctctaacatg
     1441 tgttattatt aacaaagata aatgtaagca aggaaactat cccaaagtaa atattgcgtc
     1501 actaaatgga cattggagtg acatatgatt ttattcgccg actataatac cccttatctt
     1561 ttcgctattt cctttgtact gttgatcggc cttttagaaa ttttagcgct tatctgcggt
     1621 cacatgctct ccggtgctct tgatgcacat cttgaccatt atgattccat aaccacaggt
     1681 catattagcc aggcacttca ttatctcaat atcggaagat tgccagctct cgtcgttctc
     1741 tgcttgctgg ctggtttttt tggtcttatt ggcatcctgt tgcagcatgc ctgcatcatg
     1801 gtctggcaat caccactgtc gaacctgttc gtggttcctg tcagtttact gtttacgatt
     1861 attgcggtgc attataccgg taaaatagtt gcgccctgga ttccacgcga ccatagttca
     1921 gctattacag aagaagaata tattggtagc atggcattaa ttaccggtca tcaagccact
     1981 tcaggtaatc cctgcgaagg aaaactcacc gatcaatttg gccaaataca ttatttacta
     2041 ctggagcctg aagaaggaaa atttttcact aaaggagaca aagtgttaat tatttgccga
     2101 ctttctgcaa cgcgatattt ggcggaaaac aatccctggc cacaaatatt gtaatttatt
     2161 aatcaaaagg aaatttaata atggatgata ttgttaattc tgtgccctcc tggatgttta
     2221 ccgcgattat tgccgtatgc attctgttta ttattggaat tattttcgcc aggctctatc
     2281 gtcgcgcttc ggcagagcaa gcttttgttc gtactggttt aggtgggcaa aaagtggtga
     2341 tgagcggtgg cgcaatcgtg atgccgatct ttcatgaaat aatccccatc aatatgaata
     2401 ctctgaagct ggaagtcagc cgctcaacca ttgatagcct gattacgaaa gatcgtatgc
     2461 gtgtcgatgt agtagtcgct ttctttgtac gggtaaaacc gtctgtagaa ggcatcgcca
     2521 ctgcggcgca gacgttgggg caacgcacgc tatcaccaga agacttacgt atgttggttg
     2581 aagataaatt tgtcgatgcc ctccgtgcaa cagctgcaca aatgaccatg catgagttac
     2641 aggatacccg cgagaacttc gtgcagggag tacaaaatac tgtcgcagaa gacctgtcga
     2701 aaaacggtct tgagctggaa agcgtttcac ttaccaactt taaccagacc tcgaaagaac
     2761 atttcaatcc gaacaatgcc tttgacgccg aaggtttaac caaactgact caggagacgg
     2821 agcgccgtcg tcgcgaacgt aacgaagttg aacaggatgt agaagttgcg gtgcgtgaga
     2881 aaaaccgtga tgcactttcg cgcaagttgg agattgaaca acaagaagcg tttatgacgc
     2941 ttgagcagga gcagcaggtt aaaacccgta ctgccgaaca gaatgcacgt attgcggctt
     3001 ttgaagctga acgtcgtcgt gaagcagagt agacgcgaat tctggctgaa cgacaaattc
     3061 aggaaacgga gatcgagcgc gagcaggccg tccgctcaag aaaggttgag gctgaacgtg
     3121 aagttcgtat taaagagatc gaacaacagc aggtcaccga aatcgccaac cagacgaaat
     3181 cgatcgctat tgccgccaaa tcggaacaac agtcccaggc agaagcgcgt gctaaccttg
     3241 cactcgcaga agcagtaagt gcgcaacaaa acgtagaaac cactcgccag accgcagaag
     3301 ccgatcgtgc taaacaagtt gccctaatcg ctgccgcgca ggatgcagaa accaaagcgg
     3361 ttgaactgac cgtgcgggcg aaagcagaaa aagaagccgc agagatgcag gcggcggcta
     3421 tcgttgagtt agccgaagct acacgtaaaa agggtctggc ggaagcagaa gcacaacgtg
     3481 cgctgaacga tgctatcaac gtactttctg atgaacaaac cagccttaaa ttcaaactgg
     3541 ccttgttgca ggcgctacct gcggtaatag aaaaatccgt tgagccgatg aaatctattg
     3601 acggtatcaa gattattcag gtcgatggtc tgaatcgtgg cagcgctgcg ggtgatgcaa
     3661 acacgggtaa tgtggggggc ggaaacctgg cggaacaagc attatcagcc gctctctctt
     3721 accgcacaca ggcaccgctg attgactcct tgctcaatga aattggcgtt tcaggcggct
     3781 cactggcggc attgacttca cccttaacct caacaactcc cgtcgccgaa aacgtagaat
     3841 aaattttcag ctatgaacgt ccctgtcaca aaaagccggg gattttttat atctgcgttc
     3901 cgctaaaaac tgcaaatgat caggtcgttg caacgttttg cgtgaccgct cggggaaggc
     3961 aaaactgcct ctgggaaagc gttgcgcgag gtccggcgct catcaacaat cggggagtca
     4021 gcaaagggct gaaacgggaa agcccctccc taggaagggg ccttacagaa ggaaagggtt
     4081 atgatgaagc tcgtcatcat actgattgtg ttgttaatcg taagtttcgc agcttattaa
     4141 cagccaatca gaggggggag aaatcctccc tcacccttgt tcctttactt taggttgaaa
     4201 aaaaatcagc gtcaagaagg tttcattaac acatatccct ggcccaaaaa gccggggatt
     4261 ttttatatct gcgttccgct aaaaggtgca aatgctcagg acgttgcagc gttttgcgtg
     4321 accgctcggg gaaggcaaaa ctgcttcagg gaaagcgttg cgcggggtcc ggcgctcatc
     4381 aacaatcggg gagtcagcaa ggggctgaaa cgggaaagcc cctcccgagg aaggggcctt
     4441 atagaaggaa agggttatga tgaagttcgt catcatactg gttgtgttgt tactgttaag
     4501 tttcccgact tactaacaac tgatcagagg ggggagaaat cctcccttac ccttgttcct
     4561 ttactttagg ttgaaaaaac aacagcgtca ataggcctgc ctgctacgaa gcgagatctg
     4621 tgaaccgctt tccggttagc cttttttatc ctgttggatc ttcttgatga tgttagtggt
     4681 cgagcaaccg tcttcgaagt tgagcaccag cacttcgccg ccgttggccc agacttcttt
     4741 actcccggcg atctcttctg gtttatagtc gccgcccttc accagtaaat ctggcaggat
     4801 cccggcgatc aagcgctgcg gcgtgtcttc ttcaaacgac accacccagt cgaccgcttc
     4861 cagtgcgccc agcacaatca tacgctgttc gagcgggtta acggggcggg aatccccttt
     4921 cagccgtttg gtggaggcat cgctgttcac agcgacaatc agacggtcac ccagtttgcg
     4981 agcatttgcc agataggaga cgtgcccggc gtgcaggatg tcaaagacac cgttggtcat
     5041 taccaccttt tcgccgcgtt tacgcgctgc cgctacagca agcttcagtt cctcttcggt
     5101 catcacacca aagccggtat ctgcgcgccc acgcactgca ttttccagct cgatcggcga
     5161 aaccgtggag gttcccagtt tgccgaccac cacgccagcc gccgcattgg caaagaagca
     5221 ggcttcttcc agcgaattac ccgctgccag cgttgccgcc aggacgccaa tcaccgtgtc
     5281 gcccgcaccg gtaacgtcat acacttcctg cgcctgggtt ggcatatgca gcggcgcttt
     5341 acccggttgc agcagcgaca taccctgttc ggaacgggtc actaacagag ccgagagttc
     5401 gtaatcggca atcagtttca tgccgcgctc aacaatctct tcttcggtct tacatttacc
     5461 gacaacagct tcaaattccg agagattcgg tgtcagcagc gtagcgccgc ggtagcgctc
     5521 aaaatcggta ccttttggat caatcagcac cggaacgccc gctttacgcg ccagttggat
     5581 catctgctgt acgcttgcca gcgcaccttt ggcgtagtca gaaagcacca gcgcgccaat
     5641 cgaactcagc gcctgattaa tccgctcgtg cagcggctgc ggatcaacac cttcgaaacc
     5701 ttcttcaaaa tccaggcgga tcagctgttg gttgcgggaa agtacccgta atttggtgat
     5761 agtcggatgc gtcggtacag aaacgaagtc gcatttgacg ttgacgtcgg ccagagattt
     5821 actcagcgca cgcgccgcat cgtcaatgcc cgtcagcccg accaggcgag cattggcacc
     5881 gagagaagcg atgttcattg ccacgttagc cgcgccgccc ggacgttctt cgatggtatt
     5941 gactttaacc acgggcaccg gcgcttccgg cgagatacgg ctggtgggac cgtaccagta
     6001 acgatccagc atcacatcac caaccaccat cactcctgca cgttcaaact ctggcagcgt
     6061 tactttcatt cctgtctcct gagagattca aaatttgcgc gcgataatac catacttcat
     6121 tcttccacca gccacttctg ccagcttgcc cgcaccagtt cacgctctgc ggtgaagcaa
     6181 tcctccggca catggcccgg caattcctgt aatgccagat gatgaagttc atcgcgcagc
     6241 gtagtatacg cacgggtcag cgccatcgct tcctgctctt ccataatgtc gttttgcgcc
     6301 agtagttcca gaatgcgcac gttgtctgac cagcgcgtta acttcggttt ttcatgagcg
     6361 tagcgcaaca ccagatattg ggtaataaat tcgatatcgg taattccgcc ttcatcagct
     6421 ttgatatcaa agcgatcgcg atgtttattg ccgagatgag cgcgcatttt ctcgcgcatt
     6481 tcccgcactt ccgtttgcag agttttacct tcacgcggca gcgtcataat ctcgcgacgc
     6541 accgcgtcaa agtgcgcggt gagctgcgga tcgccgtaca ctacccgcgc acgcaccagc
     6601 gcctgatgtt cccacgtcca ggcctcgttt ttctgataat cggcaaatgc ttctgcggat
     6661 gtcaccagca ttcccgcggc cccggacggg cgtagccgtg catccacttc atacaaaatg
     6721 ccggaagagg tacgcgtact gaacagatgc ataatgcgtt gcgccagacg cagataaaac
     6781 tgtcgcccgt cgatttcccg ctcgccgtca gtcatcgcat ccattgggca atcatggagg
     6841 aagatcaggt caagatcgga actgtagcct aactcccagc cgcccagctt gccgtagccg
     6901 accaccgcaa aaccacggcc ttcgcgatcg ttgaggtgat ttggcttacc gtagcgggcc
     6961 accatttgaa cccacgcctg ctgaacgacg gcatctatca tagcctctgc cagccaggtt
     7021 aagtgatcgc tcactttcat caccggtagc gtaccagcga tatccgctgc ggcgatgcgt
     7081 aacagctgtg cctgtttgaa ctgacgcagc gcctcaagct gttgctcttc gtcatcttcc
     7141 ggcacgcgca gcaaatactg gcgcaactca tcgcggtagg catcggtcgc cgtcggctgg
     7201 taaagggtgt ttggatcgag caattcatcc agcaataatg gataacgcgc cagttggctg
     7261 gcaatcatcg gcgacgcggc acacagagaa atcaaatgtt taagcgccgc ggggaattca
     7321 ctgagcaatt ctaaataggt ggtgcgggta acaatcccca ccagcaaggc ggtaatgcgc
     7381 gacagcgtaa cggcagcgtc ttcacgcgca cagacatcac ttagcagatg cggcatcaga
     7441 tggtcgagca cctgacgtcc tcgcggcccg atggtgcgct tatccagctc tttgcggaaa
     7501 tcggcaatca gcgttagcac ctgtttgcga tcatcctcgc taagatgcgc cagcactggc
     7561 gtagtgtcat cttcctgcaa cgcatcctgc cacagctcac gccactgttc cgacagcgac
     7621 tcttcctgag tttcactttc gtcgtcgcca atcaattcat taaacacccg gcgcacattg
     7681 gtcatatgtg cggtcagcgc cccggtcagt tgcggccagt cagcaaagtc catcgcccac
     7741 gccagccgcg cacggttaag ctcatcagaa ggaagcgtct gggtttgttc gtcgttaatg
     7801 ctttgcagca ggttttccag acgccgcagg aacagatacg ccactcgcaa ttgttcagca
     7861 tcgttttcag aaagcagatg cagcgcggca atgacgctga gcgttggcag taaagagcgc
     7921 gactgcagcg acggttcgcg cccaccgcga atgagctgga acacctgaac gataaattca
     7981 atttcgcgaa tgccgcctgc gccgagtttg atattgtcgg tcaaaccacg tcgacgcact
     8041 tcacgggcaa tcatcccttt catgttgcgc agcgactgaa tcacgctgaa atcgatgtaa
     8101 cgacggaaaa caaacgggcg cagcatcgca cgcaactcgt tagcatagac gccttcgcta
     8161 tcgcccataa tccgcgcctt gaccatcgcg taacgctccc agtcgcgccc ctgctcctgg
     8221 taataatcct ccaacgcggc aaagctcagc accagcgggc cactttcgcc aaacggacgc
     8281 agccgcatat ccacgcgata gacgaagcca tcctgcgttg gttgatccaa cactttaatc
     8341 agccgctgcc ccatgcgggt aaaaaactgc gcgttatcca gttcccggcg tccgccctgc
     8401 gtacaaccat gttccggcca ggcaaaaatc agatcgatat ctgaggagaa attcagttcc
     8461 ccaccgccca gcttacccat gcctaaaatc agcagcggtt gcgcttcgcc ctgcgcattg
     8521 cacggcgttc cccactcgcg gcagcaagcg tcatacagcc agtcacgcgc ggcgacaatc
     8581 agcgtctccg ccagatggct gagctgctgc aatatgctct cttcagtaac caaagccagc
     8641 gtttgcgccc aggcgatgcg caccataatg cgccgccgga atagccgcag ctcgcgcatt
     8701 aacccggctt cgtcactcac attactgagc gcctcctgca accatgccgc gtaatgctgc
     8761 cattcgtcgg cctgcggcgg ttggctttcc agttccgtca gccactctgg atgcacaatc
     8821 acgctgtcct gcacaaaatc actaaaagta agtactgact tcgcctgtgc gctaagtgat
     8881 tcctcggcta aaggctctgg cagccgctca acaacggtct gccagtactg ctgtaacggt
     8941 gaagagagcg gcttcataag cgattttatc cttgcctgat taacgttttc cgctgtgcaa
     9001 ccagaacggt tcctgattgt ttgcctcatt acggaaatgt tcaatttcaa tgcgttgccc
     9061 ggtcgcaata gcgtgacgca gcccctgcca gttctccagc caggcttgcg cgacgacagg
     9121 atcatagtaa cctgccagca gcagtattga gtcaatatca cgcgtcaggc gtggcaactg
     9181 atcatggtag cgatcgccta acggctggca gaaaacgctt ttcagttcag cggcatggcg
     9241 ggaaagatgg atatcggcaa agcgtttgaa ggaatcgctg attttaccct gcgctttggc
     9301 atctaaaaac ggctgccagg ctttgcttac caaccattcg gtcaacgcca gctttgccat
     9361 tgccgtttcg gtagagtaga ccgccgtcac ggcagaaacc gcagaagcga tggtcgcttc
     9421 gcattgagtc agcaaatcac gtaagtgagt gctcgcttta cgcggcacaa tgccaccgaa
     9481 cagcatcagt gcatggcgaa caaggccaat ggctgccagc acctgctctt tcgccgcatc
     9541 gttgccgcgt acccacagtt cttcatgata ctgccattgc gctaacgcca gctcgaacgc
     9601 cgcttccagc ccctgttcca catcggcttt tgccgcaaca tgcaaaatgg tggtcggttt
     9661 gatttcacgc gccggattgc cctgcgccag atgatagcca cgcgccgctt tgctcaggct
     9721 gccctggcgt aatccggttt gcgataccag ttggttcgcc agtttcagca ccgcgcgcgt
     9781 gtcgccgcta agcagttcca gttccagctc acagataggt tcagcaaatt cccccgcttt
     9841 cacctccccc tgatcgagag cgatttcaat tcggctgtca tcgaccgcca ccagccattt
     9901 ttcgcgataa aaatcggtgc tgaacagcgg ttgtacgcgg gaagcgagat cggcgggcaa
     9961 ttcgccgttc ggccagactt ccgtcggtaa ctgcgccagg tcgagcgtcg gttcgctcaa
    10021 cgccacgtta tattccgggc gctggtgtaa gccgcctgtc actcttcctg caactttcat
    10081 ggtcatctca tagcgaccgt tttcgccacg aatacgtaag cccatatcgt gcccacgcag
    10141 ccagttatcc ggcgtttcgt agtaaatatt cagcaactgc acggggtcat ggtgctcgcc
    10201 gcccagcgta ttgagatggt cacgcaacgc ctcaacggca ctgtgattaa caataaactt
    10261 taattcgatt tcctgagcca tggcctttac ttatcggtta tgtcacataa gtgacgatga
    10321 acggcgaact taatgcgatc ttttttgtca gtagatagta ttttgcgcca aattgccatg
    10381 caacgggcaa tttgacgggc gtaaaagttt gaagcagtgg caagacctga cacagaggat
    10441 tccgattgat gtcgaatgct ttgcgtagcg acactgatgc cactactatc gttccacttt
    10501 ccatgacaat aacgacagcc tgatgccaaa attacgcctg atcggattaa ctttactcgc
    10561 acttagcgcc actgccgtct cacacgctga agaaacgcgc tatgtttctg atgaactgaa
    10621 tacctgggtc cgtagcggtc cgggagatca ttatcgcctc gtgggcacgg ttaacgccgg
    10681 cgaggaagtg accttattac aaactgacgc caacaccaat tatgcccagg tgaaagacag
    10741 ctctggccgt accgcctgga tcccgttgaa acaacttagc actgagccaa gcctgcgctc
    10801 ccgtgtgcca gatctggaaa atcaggtcaa aaccctgacc gataaactca ccaatatcga
    10861 taacacctgg aatcagcgca ccgcagaaat gcagcaaaaa gtggcgcaga gcgacagcgt
    10921 gatcaacgga ttaaaagaag aaaatcagaa attgaaaaac gagctgattg tcgcgcagaa
    10981 aaaggtcgat gccgccagcg tacagttgga tgacaaacag cgcaccatca tcatgcaatg
    11041 gtttatgtat ggcggtggcg tgctggggct tggcttgctg ctcggtctgg tactgccaca
    11101 cctgatccca acgcgcaaac gcaaagatcg ctggatgaac taaatcgcct tcttcgttgc
    11161 actgtcttat tattaggaat tgtgtatttg acgaagggat gatggcgtga agatttatct
    11221 ggtcggtggt gctgttcggg atgcattgtt agggctaccg gtcaaagaca gagattgggt
    11281 ggtggtcggc agtacgccac aggagatgct cgacgcgggc taccagcagg taggccgcga
    11341 ttttcctgtg tttctgcatc cgctaacgca tgaagagtat gcgctggcac gtaccgaacg
    11401 gaaatccggt tccggttaca ccggttttac ttgctatgcc gcaccggatg tcacgctgga
    11461 agatgatctt aagcgtcgcg atctgaccat taatgcgctg gctcaggacg ataacggcga
    11521 gattatcgac ccgtacaacg gtctgggcga tctgcaaaat cgtctgttgc gccatgtttc
    11581 ccccgctttt ggcgaagatc cgttacgcgt tctgcgcgtg gcgcgttttg ctgcgcgtta
    11641 tgcccacctc ggttttcgta ttgccgatga aactctgacg ctgatgcgcg agatgaccca
    11701 tgcgggtgaa ctggaacacc tgacgcctga acgggtatgg aaagagacgg aaagcgccct
    11761 caccacccgc aatccccagg tgttcttcca ggtactgcgc gattgcggtg cacttcgcgt
    11821 tttattcccg gaaattgacg ccctgtttgg cgttccggcc cctgccaggt ggcatccgga
    11881 aatcgacacg ggtattcata ccttaatgac gctctcaatg gcggcgatgc tgagtccgca
    11941 ggttgatgtc cgtttcgcaa ctttatgcca cgatctcggt aaaggactga cgccgccaga
    12001 actttggccg cgtcatcatg ggcatggccc ggcgggtgtt aagttagtgg aacaactatg
    12061 ccagcgtctg cgcgtgccaa atgaaattcg cgatttagcc agactggtgg ctgagtttca
    12121 cgatctcatc cacaccttcc caatgctgaa cccaaaaacc atcgtcaaat tatttgattc
    12181 catcgatgcc tggcgtaaac cgcagcgtgt cgagcaactg gcgctgacca gcgaggctga
    12241 cgtacgcggc agaaccggtt ttgaatcagc ggactacccg caaggccgtt ggttgcgcga
    12301 agcctgggaa gtggcgcagt cagtgccgac aaaagcggtc gttgaagcgg gatttaaagg
    12361 tgtggagatt cgcgaggagc tgacccgacg gcggattgcg gcggtagcca gttggaagga
    12421 acaacgttgc ccaaagcctg actgagtata aaactaaacg ttaacacgaa ccatcccctc
    12481 gccccttcgg ggagagggtt agggtgaggg gaacagacca gcaccggtgc gaacaatcac
    12541 ccccaccccg gccctctccc tacagagcga gggagaaaac caggcctgct cccgaacctt
    12601 ttctcaatca aaagaacacg acgtacaccg cagccgccac aataaagcga taaatggcga
    12661 acgggataaa cgaaatgcgc ttaatcaatt gcaggaaggt tttaatcgct atcagcgcca
    12721 ccacaaaagc ggtgataaac ccaacggcaa acatcgggat atcgccggtt gtcaggaagc
    12781 cccagctttt gtagagatcg agcgccgttg cgcccatcat catcggtacc gccagcagga
    12841 acgagaactc ggaagcagcg taacggctca cgcccatcag catcccacct gaaatggtgg
    12901 ccccggaacg ggagaaaccc ggccacagcg ccagacactg gaaacagcca atcataaacg
    12961 cctgacgata ggtcatatca tcaagacccg gcgcacgcgg ctctttcggc ttcaggcatt
    13021 cggcggcaat cagcaacaaa ccgccaacga ccagtgcata catcacattt atcgggttaa
    13081 acaatgactt aatcgtgtcg tggaacaaca gccccagtac taccgccgga atcatcccca
    13141 gcaaaatgtg gatcagcgtt aaacgacctt tgctttctcc ttcgtgctgc aacgggcggc
    13201 caaagtggat gccaatcagg ccaaacagac gccgccagaa catcactact accgccagaa
    13261 ttgatcctaa ctggatcaca acttcaaagg ttttcgccgt gtcgccctcg aaccccaaca
    13321 agtgaccgac aataatcata tggcccgtgc tggataccgg cagaaattct gtcaatcctt
    13381 cgaccacacc caatattgcc gctatcagca gcgagtgcat atcgctcatc aataaacccc
    13441 taaattatta aaatgtaccg cttgtccgaa ctactgcgta tgaccaggtt ataaccgttt
    13501 ggtttaacag ctgtaaaatt aattattttc tttcagatta ttgccacgct caatgattac
    13561 gccaacattc gccgcccgcg ccactgcgcc tggcttgctg agtttgatac gcacccacgg
    13621 cgagttaaag cgtgctaaca gcagctccgc cacctcttca gccacgcgtt ccaccagcgc
    13681 aaaacgcgcc ccctcgacgt ggctgaccac cgtttctgca atgtcagcgt aactgaggca
    13741 atccgccaca tcatcacttt tcgccgcttt acggttatcc catgccattt cgatatcgaa
    13801 cactaacttc tgttcgatgg tctgttccca gtcgtaaaca ccaatagtgg tgattaccga
    13861 aagttgctct ataaatacaa tatccatcac gtcctgcctg ctttttggct aaccggatac
    13921 cacttccggc gaaatgtgcg tattatccac agattcatcg ttgaacacga attttcaaaa
    13981 cggaacagct tatgagtgca atcgcgcctg gaatgatcct catcgcgtac ctctgcggct
    14041 ccatttccag tgccattctg gtttgccgct tgtgtgggct gcccgatccg cgaaccagcg
    14101 gctccggcaa tccaggcgca accaatgtgt tacgtatcgg tggcaaggga gcagccgtag
    14161 cagtactgat tttcgacgtt ctgaaaggaa tgttgcccgt ctggggcgcg tatgaattag
    14221 gtgtcagccc cttctggcta ggcttaattg ccatcgccgc ctgtcttgga cacatctggc
    14281 ccgttttctt cggatttaaa ggaggaaaag gcgttgctac cgcttttggt gccatcgcac
    14341 ccattggctg ggatctcacc ggagtaatgg cgggaacctg gttactgacc gtgctattga
    14401 gcggatactc gtcgctggga gcgattgtca gtgcactgat tgctccgttt tatgtatggt
    14461 ggtttaagcc acaattcacc ttcccggttt cgatgctctc ttgcctgatc ctgctgcgtc
    14521 atcatgacaa catccagcgt ctgtggcgtc gtcaggagac aaaaatctgg acgaaattca
    14581 aaagaaagcg cgaaaaggat cccgagtgat ttctggtgga tctacatgac ctgatagcct
    14641 tcatcgggct tgcccagccg ttgctggcac catgccgcca gaaattccac gcagacacgt
    14701 aatttcatgc tgcgatagag cggctcccgg taaacagccc agatattggc gctttgtgca
    14761 tactccggca atacttgcac cagtttgcca ctctccagaa acggcaacac atcccactcg
    14821 gaacgcagca taatcccttt gccctccagc gcccattgca acactatctc gccgctattg
    14881 gaggaaagat gcccgctaac cttcaccgat tttttctcct gaccgttccc caactcccat
    14941 attccgtggg tcatatcgcg ctctttggtc accagacagt catgacgact taattcctgt
    15001 aatgattgtg gctgcggata tttttgcaga tactcaggtg ctgcgcataa tattctttta
    15061 tttttcgtta acagatgcgc aatataataa tcaggaattt cgtcattaat acgaatatcc
    15121 agatcaatat tatcctgcac taaatcaatt tgccgatcga acaattcaaa atgcacctgt
    15181 aactcaggat aattgcgcat aagttcggta atggctggcg caatatggct gcgcccaaaa
    15241 ccaaaactgc aaccaatacg aatcatcccc tccgggcgcg ttttgatttg cgtgacgtca
    15301 tcgaccagtc gctgatattg ggtgaggatt tccagcgcat gttcgtaaca acgttggcca
    15361 ctttcagtca gcgccacgcc gcgagcggag cggttcagca gcgttgttgc cagcgtattt
    15421 tcaaggattt ggatccgctt ggtgacaaat gcaggcgtct gcccaagcgt cgccgctgca
    15481 gcactaaaac tcccgctgtg gacgatttct accagtacct gcaagtcttt ggctaaaggc
    15541 cagctattca gcatcgttaa ttatccgcag ttgtgataag cgcagtgtat ttcgcaaaac
    15601 attgccattc cctgctctgg cgcaatattc attcttattt tcgcgttgat tcacgaactg
    15661 ttaattacat attcacggtg gcaaaaaata taaaaccaca tttttagtgg tagtttgtgg
    15721 cggtgaattt ttccagacaa atacaaaaac tggagttgcc atgatgagcg aaagtaataa
    15781 gcaacaggca gtgaataagt tgacagagat tgtcgctaac tttaccgcca tgatttctac
    15841 ccgaatgcct gatgacgtgg tggataaact aaaacagcta aaggatgccg aaacgtcgtc
    15901 gatggggaaa attatctacc atacgatgtt cgacaacatg caaaaagcga ttgacctgaa
    15961 tcgtcctgcc tgtcaggaca ccggggagat tatgttcttc gttaaagtcg gttcccgctt
    16021 cccactgctt ggcgagctgc aaagcatact caaacaagcc gtggaagagg caaccgtcaa
    16081 agcgccacta cgtcacaatg cggtagaaat ttttgacgaa gtaaacaccg gcaaaaatac
    16141 cggtagcggc gtaccgtggg tcacctggga catcatcccc gacaatgacg atgcggaaat
    16201 cgaagtttac atggcaggcg gcggctgcac gctacctggc cgctcgaaag tgttaatgcc
    16261 gtcagaaggc tacgaaggcg tggtgaaatt cgtcttcgaa aatatctcca ccctcgccgt
    16321 aaacgcctgt ccaccggtac tggtgggcgt gggcatcgcc acctcggtgg aaaccgccgc
    16381 cgtgctctcg cgaaaagcca ttttgcgccc gattggcagc cgccatccca atccaaaagc
    16441 ggcagagctg gagctacgcc tggaagaagg actcaaccgt ctgggaattg gcccacaagg
    16501 gctgactggc aacagttcag tgatgggcgt gcatatcgaa tctgccgccc gccatccgtc
    16561 aaccatcggc gttgctgttt ctacaggctg ctgggcgcat cgtcgcggca cactgctggt
    16621 tcatgccgat ctcacctttg aaaatctgtc tcacacccgg agcgcgttat gaaaaagatc
    16681 ctgacaaccc cgatcaaagc tgaagatctg caagatattc gcgtcggcga tgtgatctac
    16741 ctgaccggta cgctggtgac ctgccgtgac gtttgtcacc gccgcttgat cgaactgaaa
    16801 cgtccgatcc cttacgatct caacggcaaa gcgattttcc acgctggccc catcgtgcgc
    16861 aaaaacggcg ataaatggga gatggtttcc gttggcccga caaccagtat gcgtatggaa
    16921 agttttgaac gtgaatttat cgagcagacc ggcgtgaaac tggtggttgg caaaggtggt
    16981 atggggccgc tgaccgaaga aggctgccag aaattcaagg cgctacatgt gattttcccg
    17041 gcaggctgcg cggtgctggc ggcaacccag gtagaagaga ttgaagaagt gcactggaca
    17101 gaactcggga tgccggagtc actgtgggtc tgccgggtca aagagttcgg cccgctgatt
    17161 gtctctattg atacccacgg caacaacctg atagccgaaa acaaaaagct gttcgccgaa
    17221 cgccgcgatc ccatcgtgga agagatctgc gagcacgtcc attacatcaa ataaccctcc
    17281 cggagaggct cacccctctc ctttttcgca ggcataacac gatgaaacct tccactgaat
    17341 ggtggcgata tcttgcgccg ctggcggtca tcgccattat tgctctactt ccggttcccg
    17401 cagggctgga gaaccatacc tggctctact ttgccgtttt tactggcgtg atcgttggac
    17461 tgatcctcga acccgtgccg ggtgccgtgg tggcgatggt gggtatctcc atcatcgcca
    17521 tactctctcc ctggctgctg ttcagccccg aacagctcgc ccagccaggc tttaaattca
    17581 ctgcaaaatc cctctcgtgg gcggtttccg gtttttctaa ttcggttatc tggctgattt
    17641 tcgccgcctt tatgtttggc acaggctatg aaaaaaccgg gcttggacgc cgcattgcgc
    17701 tgtttctggt gaaaaagatg ggacatcgca cgctgtttct cggctatgcg gtgatgttct
    17761 ccgagctgat cctcgcacct gtaacaccgt ccaactcggc gcgtggtgcg gggattatct
    17821 atcccatcat ccgtaacctg ccaccgctct atcaatcaca accaaacgac agcagttcgc
    17881 gcagcattgg ctcgtacatc atgtggatgg ggattgttgc cgactgcgtg accagcgcca
    17941 ttttcttgac ggcgatggca ccaaacttgc tgttaattgg actgatgaaa agcgcatctc
    18001 acgccacgct gagttggggc gactggttcc tcgggatgtt gccgctcagt attttgctgg
    18061 tgctgctggt tccctggctg gcttatgtgc tgtacccgcc ggtactgaag tctggcgatc
    18121 aggtgccgcg ctgggcagag acggaactgc aggcaatggg cccgctctgt tcgcgtgaaa
    18181 aacggatgct ggggctgatg gtaggcgcgc tggtgctgtg gattttcggc ggtgattata
    18241 tcgatgccgc gatggtcggt tacagcgtgg tggcactgat gctgcttctg cgcattatca
    18301 gttgggacga cattgtcagt aataaagcgg cgtggaacgt tttcttctgg ctggcctcgc
    18361 ttatcaccct cgctaccgga ctcaacaaca ctggttttat tagctggttt ggcaaactgt
    18421 tagcaggcag cttaagcggt tattcgccaa cgatggtgat ggtggcgttg attgtggtgt
    18481 tttatctgct gcgctacttt ttcgccagcg ccacggcgta tacctccgcc ctcgcaccga
    18541 tgatgattgc cgctgcgctg gcgatgccgg aaatcccgct gccggtgttc tgcctgatgg
    18601 ttggcgcggc aattggtctg gggagcattc ttacaccata cgccaccgga cccagtccga
    18661 tttactacgg tagtggttat ctgccaacgg tggattactg gcgactgggg gcgatttttg
    18721 gactgatatt cctcgtgttg ctggtgatta ccggcttgct gtggatgccc gtggtgttgc
    18781 tttaagtgag gacggtgccg gaaattgctc tccggcaccg gatagtttta cgcagccggt
    18841 aactctgcca gtggccagcg cggacgcacg ctaacgccga gatccgccgt cgcgcctgct
    18901 ttaaaccgca ccattccggc ataggcgatc atcgcgccgt tatcagtaca aaattccgga
    18961 cgcgcgtaga acacttcgcc gcggcgtttt ttcatcattt ccgccagctt cgcccgtaac
    19021 gtgcggttag cactcacgcc gcccgccatg accagtcgct taaagcccgt ctgatccaac
    19081 gctcgcttac acttaatcat caacgtatcg accaccgcat cttcaaaggc gcgggcgata
    19141 tcagcacgcg tctggtcgtc ggtgccgttg tcacgaatcg tgttcgccgc aaaggttttc
    19201 agaccagaaa agctgaaatc cagccccgga cggtcggtca tcggacgcgg gaaaacaaag
    19261 cgcccggcag taccctgagc cgccattttc gacagtaacg gtccgccagg ataatccagc
    19321 cccagcagct tcgcggtttt atcaaacgct tcaccggcgg catcatcgat agactcgccg
    19381 agcagctcgt actgaccaat gccagtcacg ctgattaact gcgtatgacc gccggacacc
    19441 agcagcgcga caaacggaaa ttccggcggg ttatcttcca gcatcggcgc taacagatgc
    19501 ccttccatat ggtgtaccgg gattgccgga acgttccagg caaacgccag agaacgcccc
    19561 acggttgcgc caaccagcag tgcgccgact aatccagggc ctgcggtata ggccacagca
    19621 tcaatgtctt ttgccgttaa accagactcc tttagcgccg cctggatcaa cggtacggtt
    19681 ttgcgcacgt gatcgcggga ggccagttca ggcacgacgc cgccgtagtc agcgtgcaat
    19741 ttcacctgac tatacaattg gttggctaac aaaccttttt catcgtcgta aatggcgatg
    19801 ccggtttcat cgcaggaagt ttcaataccc agtacacgca tgacttgttt tacctcgctt
    19861 tattaccgcg cagtgtagga ccaatgcggg ttgatgtaaa actttgttcg cccctggaga
    19921 aagcctcgtg tatactcctc acccttataa aagtcccttt caaaaaaggc cgcggtgctt
    19981 tacaaagcag cagcaattgc agtaaaattc cgcaccattt tgaaataagc tggcgttgat
    20041 gccagcggca aaccgaatta atcaaaggtg agaggcacat gccggtaatt aaagtacgtg
    20101 aaaacgagcc gttcgacgta gctctgcgtc gcttcaagcg ttcctgcgaa aaagcaggtg
    20161 ttctggcgga agttcgtcgt cgtgagttct atgaaaaacc gactaccgaa cgtaagcgcg
    20221 ctaaagcttc tgcagtgaaa cgtcacgcga agaaactggc tcgcgaaaac gcacgccgca
    20281 ctcgtctgta ctaattcccc gagagcgttg ctctccgatc agaccgagtt gtagttgtaa
    20341 ggccgtgctt ccgaaaggaa tgcgcggctt attttcgttt atgaattgct aaaaatcggg
    20401 gcctatggct ggacgaatcc cacgcgtatt cattaatgat ctgctggcac gcactgacat
    20461 cgtcgatctg atcgatgccc gtgtgaagct gaaaaagcag ggcaagaatt tccacgcgtg
    20521 ttgtccattc cacaacgaga aaaccccgtc attcaccgtt aacggtgaga aacagtttta
    20581 ccactgcttt ggatgtggcg cgcacggcaa cgcgatcgac ttcctgatga actacgacaa
    20641 gctcgagttc gttgaaacgg tcgaagagct ggcagcaatg cacaatcttg aagtgccatt
    20701 tgaagcaggc agcggcccca gccagatcga gcgccatcag aggcaaacgc tttatcagtt
    20761 gatggacggt ctgaatacgt tttaccaaca atctttacaa caacctgttg ccacgtctgc
    20821 gcgccagtat ctggaaaaac gcggattaag ccacgaggtt atcgctcgct ttgcgattgg
    20881 ttttgcgccc cccggctggg acaacgtcct gaagcggttt ggcggcaatc cagaaaatcg
    20941 ccagtcattg attgatgcgg ggatgttggt cactaacgat cagggacgca gttacgatcg
    21001 tttccgcgag cgggtgatgt tccccattcg cgataaacgc ggtcgggtga ttggttttgg
    21061 cgggcgcgtg ctgggcaacg atacccccaa atacctgaac tcgccggaaa cagacatttt
    21121 ccataaaggc cgccagcttt acggtcttta tgaagcgcag caggataacg ctgaacccaa
    21181 tcgtctgctt gtggtcgaag gctatatgga cgtggtggcg ctggcgcaat acggcattaa
    21241 ttacgccgtt gcgtcgttag gtacgtcaac caccgccgat cacatacaac tgttgttccg
    21301 cgcgaccaac aatgtcattt gctgttatga cggcgaccgt gcaggccgcg atgccgcatg
    21361 gcgagcgctg gaaacggcgc tgccttacat gacagacggc cgtcagctac gctttatgtt
    21421 tttgccggat ggcgaagacc ctgacacgct ggtacgaaaa gaaggtaaag aagcgtttga
    21481 agcgcggatg gagcaggcga tgccactctc cgcatttctg tttaacagcc tgatgccgca
    21541 agttgatctg agtacccctg acgggcgcgc acgtttgagt acgctggcac tgccattgat
    21601 atcgcaagtg ccgggcgaaa cgctgcgaat atatcttcgt caggaattag gcaacaaatt
    21661 aggcatactt gatgacagcc agcttgaacg attaatgcca aaagcggcag agagcggcgt
    21721 ttctcgccct gttccgcagc taaaacgcac gaccatgcgt atacttatag ggttgctggt
    21781 gcaaaatcca gaattagcga cgttggtccc gccgcttgag aatctggatg aaaataagct
    21841 ccctggactt ggcttattca gagaactggt caacacttgt ctctcccagc caggtctgac
    21901 caccgggcaa cttttagagc actatcgtgg tacaaataat gctgccaccc ttgaaaaact
    21961 gtcgatgtgg gacgatatag cagataagaa tattgctgag caaaccttca ccgactcact
    22021 caaccatatg tttgattcgc tgcttgaact gcgccaggaa gagttaatcg ctcgtgagcg
    22081 cacgcatggt ttaagcaacg aagaacgcct ggagctctgg acattaaacc tggagctggc
    22141 gaaaaagtga tttaacggct taagtgccga agagcgatcg ggaagccccc gacagccgca
    22201 ctgagaggca gcggcaaata tataagtacg ccctcgtaat tatcgttggc ggtaaacaac
    22261 cgttggattt cagcgttaac ggctgaagga catcgggtca atcgcccaac accaacctca
    22321 tgaaataagt gtggataccg tcttatggag caaaacccgc agtcacagct gaaacttctt
    22381 gtcacccgtg gtaaggagca aggctatctg acctatgccg aggtcaatga ccatctgccg
    22441 gaagatatcg tcgattcaga tcagatcgaa gacatcatcc aaatgatcaa cgacatgggc
    22501 attcaggtga tggaagaagc accggatgcc gatgatctga tgctggctga aaacaccgcg
    22561 gacgaagatg ctgccgaagc cgccgcgcag gtgctttcca gcgtggaatc tgaaatcggg
    22621 cgcacgactg acccggtacg catgtacatg cgtgaaatgg gcaccgttga actgttgacc
    22681 cgcgaaggcg aaattgacat cgctaagcgt attgaagacg ggatcaacca ggttcaatgc
    22741 tccgttgctg aatatccgga agcgatcacc tatctgctgg aacagtacga tcgtgttgaa
    22801 gcagaagaag cgcgtctgtc cgatctgatc accggctttg ttgacccgaa cgcagaagaa
    22861 gatctggcac ctaccgccac tcacgtcggt tctgagcttt cccaggaaga tctggacgat
    22921 gacgaagatg aagacgaaga agatggcgat gacgacagcg ccgatgatga caacagcatc
    22981 gacccggaac tggctcgcga aaaatttgcg gaactacgcg ctcagtacgt tgtaacgcgt
    23041 gacaccatca aagcgaaagg tcgcagtcac gctgccgctc aggaagagat cctgaaactg
    23101 tctgaagtat tcaaacagtt ccgcctggtg ccgaagcagt ttgactacct ggtcaacagc
    23161 atgcgcgtca tgatggaccg cgttcgtacg caagaacgtc tgatcatgaa gctctgcgtt
    23221 gagcagtgca aaatgccgaa gaaaaacttc attaccctgt ttaccggcaa cgaaaccagc
    23281 gatacctggt tcaacgcggc aattgcgatg aacaagccgt ggtcggaaaa actgcacgat
    23341 gtctctgaag aagtgcatcg cgccctgcag aaactgcagc agattgaaga agaaaccggc
    23401 ctgaccatcg agcaggttaa agatatcaac cgtcgtatgt ccatcggtga agcgaaagcc
    23461 cgccgtgcga agaaagagat ggttgaagcg aacttacgtc tggttatttc tatcgccaag
    23521 aaatacacca accgtggctt gcagttcctt gacctgattc aggaaggcaa catcggtctg
    23581 atgaaagcgg ttgataaatt cgaataccgc cgtggttaca agttctccac ctacgcaacc
    23641 tggtggatcc gtcaggcgat cacccgttct atcgcggatc aggcgcgcac catccgtatt
    23701 ccggtgcata tgattgagac tatcaacaaa ctcaaccgta tttctcgcca gatgctgcaa
    23761 gagatgggcc gtgaaccgac gccggaagaa ctggctgaac gtatgctgat gccggaagac
    23821 aagatccgca aagtgctgaa gatcgccaaa gagccaatct ccatggaaac gccgatcggt
    23881 gatgatgaag attcgcatct gggggatttc atcgaggata ccaccctcga gctgccgctg
    23941 gattctgcga ccaccgaaag cctgcgtgcg gcaacgcacg acgtgctggc tggcctgacc
    24001 gcgcgtgaag cgaaagttct gcgtatgcgt ttcggtatcg atatgaacac cgaccacacg
    24061 ctggaagaag tgggtaaaca gttcgacgtt acccgcgaac gtatccgtca gatcgaagcg
    24121 aaggcgctgc gcaaactgcg tcacccgagc cgttctgaag tgttgcgtag cttcctggac
    24181 gattaatcgg taggccggat caggcgttac gccgcacccg gcactagtcc ctttgcacaa
    24241 acgccacctt ttcggtggcg ttttttatcg cccacgcact accagcgcct ggtccagctc
    24301 gcgatatgct tcaaccagtt tctctagtga aacgcgactt aaaccgctgg gatttggcag
    24361 cacccaaatc tgcgtcgaac caatggtgag cgtttgtttc ccccactgtg caccgcgctg
    24421 gctgaatccc tgttcatatg cttgtttgcc cagaatcgcc aacgcctgcg gctgataatc
    24481 ttcaattttt tcaatcagct tacgcccacc tgcgtgcagc tcctgctttg aaacttcatt
    24541 ggcttgcacc gttggacggt ctaccagttt ggtgacgcca caacgataat ccagcagatg
    24601 ctgtgcctcc tgcggcttca actgacggtc ggtaaacccg gcctgatata tcaccttcca
    24661 gaagcgattt gctggatgag caaagggaaa accagtcccg gcggatgaaa gcccagggtt
    24721 gataccgcaa aacacgaccc gtaaccctag agccaaaata tcctcaacca tatttactcc
    24781 tttctaacat caccaggaaa agtataacgg attgaaaata cattgtttat aaaaacagca
    24841 ggcgcacggt aatggctgga ttgcgacacg gagtta
//
LOCUS       DAZGLJ010000050        24618 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000050 DAZGLJ010000000
VERSION     DAZGLJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.050"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            159..1364
                     /gene="gltS"
                     /locus_tag="ACHK49_001820"
     CDS             159..1364
                     /gene="gltS"
                     /locus_tag="ACHK49_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO9262479.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            1367..2233
                     /locus_tag="ACHK49_001821"
     CDS             1367..2233
                     /locus_tag="ACHK49_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9262480.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(2218..4299)
                     /gene="recG"
                     /locus_tag="ACHK49_001822"
     CDS             complement(2218..4299)
                     /gene="recG"
                     /locus_tag="ACHK49_001822"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO9262481.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVHHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(4305..4994)
                     /gene="trmH"
                     /locus_tag="ACHK49_001823"
     CDS             complement(4305..4994)
                     /gene="trmH"
                     /locus_tag="ACHK49_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO9262482.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(5001..7109)
                     /gene="spoT"
                     /locus_tag="ACHK49_001824"
     CDS             complement(5001..7109)
                     /gene="spoT"
                     /locus_tag="ACHK49_001824"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO9262483.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(7128..7403)
                     /gene="rpoZ"
                     /locus_tag="ACHK49_001825"
     CDS             complement(7128..7403)
                     /gene="rpoZ"
                     /locus_tag="ACHK49_001825"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO9262484.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(7458..8081)
                     /gene="gmk"
                     /locus_tag="ACHK49_001826"
     CDS             complement(7458..8081)
                     /gene="gmk"
                     /locus_tag="ACHK49_001826"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO9262485.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            8339..10021
                     /gene="ligB"
                     /locus_tag="ACHK49_001827"
     CDS             8339..10021
                     /gene="ligB"
                     /locus_tag="ACHK49_001827"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO9262486.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(10018..10635)
                     /locus_tag="ACHK49_001828"
     CDS             complement(10018..10635)
                     /locus_tag="ACHK49_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO9262487.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(10927..11751)
                     /gene="dinD"
                     /locus_tag="ACHK49_001829"
     CDS             complement(10927..11751)
                     /gene="dinD"
                     /locus_tag="ACHK49_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO9262488.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(11972..12835)
                     /locus_tag="ACHK49_001830"
     CDS             complement(11972..12835)
                     /locus_tag="ACHK49_001830"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO9262489.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            12962..13678
                     /gene="rph"
                     /locus_tag="ACHK49_001831"
     CDS             12962..13678
                     /gene="rph"
                     /locus_tag="ACHK49_001831"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO9262490.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            13744..14385
                     /gene="pyrE"
                     /locus_tag="ACHK49_001832"
     CDS             13744..14385
                     /gene="pyrE"
                     /locus_tag="ACHK49_001832"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO9262491.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(14422..15018)
                     /gene="slmA"
                     /locus_tag="ACHK49_001833"
     CDS             complement(14422..15018)
                     /gene="slmA"
                     /locus_tag="ACHK49_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO9262492.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(15125..15583)
                     /gene="dut"
                     /locus_tag="ACHK49_001834"
     CDS             complement(15125..15583)
                     /gene="dut"
                     /locus_tag="ACHK49_001834"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO9262493.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(15561..16781)
                     /gene="coaBC"
                     /locus_tag="ACHK49_001835"
     CDS             complement(15561..16781)
                     /gene="coaBC"
                     /locus_tag="ACHK49_001835"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO9262494.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            16953..17621
                     /gene="radC"
                     /locus_tag="ACHK49_001836"
     CDS             16953..17621
                     /gene="radC"
                     /locus_tag="ACHK49_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO9262495.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            17838..18074
                     /gene="rpmB"
                     /locus_tag="ACHK49_001837"
     CDS             17838..18074
                     /gene="rpmB"
                     /locus_tag="ACHK49_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO9262496.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            18095..18262
                     /gene="rpmG"
                     /locus_tag="ACHK49_001838"
     CDS             18095..18262
                     /gene="rpmG"
                     /locus_tag="ACHK49_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO9262497.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            18360..19169
                     /gene="mutM"
                     /locus_tag="ACHK49_001839"
     CDS             18360..19169
                     /gene="mutM"
                     /locus_tag="ACHK49_001839"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO9262498.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(19208..19687)
                     /gene="coaD"
                     /locus_tag="ACHK49_001840"
     CDS             complement(19208..19687)
                     /gene="coaD"
                     /locus_tag="ACHK49_001840"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO9262499.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(19695..20972)
                     /gene="waaA"
                     /locus_tag="ACHK49_001841"
     CDS             complement(19695..20972)
                     /gene="waaA"
                     /locus_tag="ACHK49_001841"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO9262500.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            21385..22443
                     /gene="rfaQ"
                     /locus_tag="ACHK49_001842"
     CDS             21385..22443
                     /gene="rfaQ"
                     /locus_tag="ACHK49_001842"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO9262501.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            22440..23564
                     /locus_tag="ACHK49_001843"
     CDS             22440..23564
                     /locus_tag="ACHK49_001843"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO9262502.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVCSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAPYIVEANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARYYADTQDLYSLPEKAADIITGGLDG"
     gene            23557..24354
                     /gene="rfaP"
                     /locus_tag="ACHK49_001844"
     CDS             23557..24354
                     /gene="rfaP"
                     /locus_tag="ACHK49_001844"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO9262503.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMHGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            24397..>24618
                     /locus_tag="ACHK49_001845"
     CDS             24397..>24618
                     /locus_tag="ACHK49_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO9262504.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYF"
BASE COUNT         5782 a         6067 c         6617 g         6152 t
ORIGIN      
        1 ggtatttttc tgcttaaaat ccatgccatt tgttcattaa cagctgagaa tttgacccag
       61 gaaagcataa ctcgataccc gtcatagttc atgaagttgc cactgcgcga tgaagtatga
      121 cgagtatgaa agagtgatgc ggatacaaag gagtaactat gtttcatctc gatactttag
      181 caacgcttgt tgccgcaacg ctgacgttgc tgctcgggcg taagttggtc cattccgtct
      241 cctttttgaa gaaatacacc ataccggaac ctgttgcggg tggtttgttg gtggcgctgg
      301 cgctactagt actgaaaaaa agcatgggct gggaagtcaa ctttgatatg tccctgcgcg
      361 atccgttaat gctggctttc ttcgccacca ttggcctgaa cgccaacatt gccagtttgc
      421 gtgccggtgg gcgtgtggtt ggcatcttct tgattgtggt tgttggtctg ttggtgatgc
      481 aaaatgccat tggcattggt atggctagcc tgttagggct tgatccgctg atggggctgt
      541 tggccggttc tattacgctt tccggcggtc acggtacggg cgctgcgtgg agtaaattgt
      601 tcattgaacg ttatggcttc accaatgcga cggaagtggc gatggcctgt gcaacgttcg
      661 gtctggtgct gggcggcttg attggcggtc cggtggcgcg ctatctggtg aaacactcca
      721 ccacgccgaa cggtattccg gatgaccagg aagtcccgac ggcgtttgaa aagccggatg
      781 tgggacgcat gatcacctcg ctggtgctga ttgaaactat cgcgctgatt gctatctgcc
      841 tgacggtggg gaaaattgtt gcgcaacttt tggctggcac tgcttttgaa ctgccgacct
      901 tcgtctgtgt actgtttgtt ggcgtgattc tgagcaacgg tctgtcaatg atgggctttt
      961 accgcgtctt tgagcgtgcg gtatccgtgc tgggtaacgt aagcctgtcg ttgttcctgg
     1021 cgatggcgct gatggggctg aaactgtggg agctggcttc gctggcgctg ccgatgctgg
     1081 cgattctggt ggtacagacc atcttcatgg cgttgtatgc catcttcgtt acctggcgca
     1141 tgatgggcaa aaactacgat gcggcagtgc tggctgcggg tcactgtggt tttggcctcg
     1201 gtgcaacgcc aacggcaatc gccaacatgc aggcgatcac tgaacgcttt ggcccgtcgc
     1261 acatggcgtt tttggtggtg ccgatggtcg gtgcgttctt tatcgatatc gtcaatgcgc
     1321 tggtgattaa gctgtattta atgttgccga tttttgccgg ttaaaaatga aaaagcgtcg
     1381 gaaggaaccg gaaacgctac gtgaacactg tcgccatatt tttggtgatg agccgccagt
     1441 gctctgtgta tgggaaacgg aatttgatta cgcagatgcc gaactcaagg cgctggcggc
     1501 taaagagtgg cagcagatca gcgaatggga tttgagtgct tactatgtac tcaatctcgt
     1561 ttataacgaa ccgatgcaaa tagagttgtt tcgctatctg tttccgctct gcctggcgca
     1621 atggcatgaa accgttttag ctggcggtta tggtgatcac ttcgaagaat ccttaatgaa
     1681 ggcattgtgt cggccctatc tctggcagga gatgatgaat gcttcccaac gccagcaggt
     1741 acggcaattt ttactggaca ccgcgttgca gcgtatggat aacgagcgtg gtttcaataa
     1801 cgttctttgc tggctggcgg tgttcaatac tctgggtggg gccgcgccgt taattcgttc
     1861 tctctggtcg cgctggtggg cgctggatac gccaggaaaa gcggtgtgtg ctattcagta
     1921 tgccgcgcat ctaatttatc ccatagaagc caatccgtta tggtcgcaag aatggatagg
     1981 ctgggggcat ccattaggcc ataaagatgg ctggtcctct gataatcgcg cttttcttcg
     2041 ccaaatgtta acacctgaaa tgattgtggc tggcgtacag gcagcggctg aaattctgcg
     2101 tggtgaaccg gaaggcgcaa tggcggcgcg aattgcacag gatgcgtatg aggcaatgga
     2161 tatcctcact atccagattg aagatttact gcgtgacttg tcttgtgatg agtctggtca
     2221 tgcgttggag taacgttccg tctccggcat ccagcgttct atcagggctt ttgcttgttg
     2281 tgggtaacgt tcgtgaatat gtcgcgccag gcgctgaact tccgggatca tcgcctgatc
     2341 gcgcagtaaa tccgccactt taaactcagc attacccgtc tgacgcgtac ctaacagttc
     2401 acccggtccg cgaatctcta agtctttctg tgcaatcaca aaaccgtcgt tgctgtcgcg
     2461 cagcacttgc agccgaattt gcgcggtttt ggagagcggc gttttgtaga gcagcacgca
     2521 gtgagaagcc accgcaccac gacctacacg cccgcgaagc tggtgtaact gcgccagacc
     2581 cagacgctcc gggttttcga taatcatcag actggcgtta ggcacatcaa cgccgacttc
     2641 aataacggtt gtggcaacca gcaggtgtag ctcaccttgt ttaaacgacg ccatcaccgc
     2701 ctgtttctcg gcaggtttca tccgcccgtg taccaggcca acgttcaact ctggtagcgc
     2761 cagtttcaac tcttcccagg tagcttccgc cgcctgcgct tccagcaatt ccgactcttc
     2821 aatcaacgta caaacccagt atgcctgacg accttcagtt atgcaggcgt ggtgcacgcg
     2881 gtcaatgatg tcggtacggc gggtatcagg aatagcgacc gtagtcactg gcgtgcggcc
     2941 tggcggcagc tcatctatca ccgaggtatc gagatcggca tacgcagtca ttgccagcgt
     3001 gcgggggatc ggcgtggcgg tcatgatcaa ctgatgcgga tggaagccct gctgctggcc
     3061 tttctcccac aatgccagac gctgatgcac gccaaaacga tgctgttcgt cgataatcac
     3121 cagcgccagg ccgttaaact gcacctgttc ctggaagatg gcgtgtgtac cgacaatcat
     3181 ctgcacctga ccgctggcga tggcttcctg ctgtgccagc cgtgctttac ctttctgctt
     3241 accggcgagc cagcccactt cgataccgag cggtgcaaac cagttgcgga agttattggc
     3301 gtgctgctcg gcgagtaatt cggttggtgc catcaatgct acctgtttgc cgtgggcaat
     3361 cgcacgcaac gcggcgaggg cggcgaccag cgttttaccg gaacctacat cgccctgcac
     3421 cagacgcatc atcggcacat ccagcgccat atcgcgctcg atctccgcca ctacgcgtgc
     3481 ctgtgcgccc gttggcttga acggtaaggc ggcgaggagt ttatttttca gcgtgtcatt
     3541 ggcgctcagc ggctgggcat gaaaacgctg tgctccggca cgtaaggcta acatgctgag
     3601 gttgtgcgcc agcagttctt ccagaatcag acgacgttgc gccggatgct gcccggtttc
     3661 cagatcgcta agctgtagcg tcggtggcgg gcggtgcaaa gtgcgcaacg cttccggtag
     3721 cgtcatcatt ccttgtgaca gttccggcgg caggagttct tcaatggcgc aggtgtcgag
     3781 cagatccagc gcctggtcgg ttaatttacg cagcgtggcc tgctttacgc cttccgttgt
     3841 tggataaacc ggcgtgagcg tttcctgtaa ttctggcgtg ctgagatcgc cctgcacgcg
     3901 gtattccggg tggatcatct ccgcaccata tttaccgcgc tttgcttcgc cataagccag
     3961 tacacggcgg cccgccgcca ggctattttt cattgccgcg ctgaaattga aaaagcgcat
     4021 ggtgaggatg ccggaaccgt cgctgatctg gcaggtcatc atccgccgac cgccgaagga
     4081 gatattgcag ttcagcactt cgccttccac cgtggcataa acgcccggca gtagttctcc
     4141 gatggggtag agatgggtgc gatcttcgta gcgcagggga aggtgtaaga gtagatcctg
     4201 cacggtatgc aggttgattt tcgccagttt gttactaagt gctgcgccaa cgcccgttag
     4261 ggaactgagt gggacagcat ctaacaggcg acctttcatg gcacttaccc tgcagcctgc
     4321 atagtagccc accagtcggc atcagcttcg atctcgcctt gctgattgac gtggggataa
     4381 ggcaggcctt tgcgttttgc gactttcgcc agcaccggat agccgccttc aaacaacagg
     4441 cgttgttgct ctgcttccgg caacatgctg ttttcacgca ggtacatgcc tgcattttgc
     4501 cgctgacgct gggcttcgta aagaatgagg gctgaggcaa cggacacatt cagcgactgc
     4561 accatgccga tcatcggaat gatgatgtcc tgatccgcca gggccaatgc ttcctgcgtg
     4621 atgcccgttt tctcctgtcc catcaaaatg caggtcgggc gagtgtaatc aatttcgcgg
     4681 aaatcgacag cgttatcaga aagatgggtt gccagaatct gcatgccctg gcctttgaga
     4741 tgagcgacgg catcgccaat ggtgcggtgt gttttcacct gtacccagct gttactaccc
     4801 gccgctgccg aagccatggt gcgcatgcgg ctaccaggcc agacggcgtg aacttcatgt
     4861 acgccaacgg catctgcggt acgaataatc gcagaaacgt tatgaggttt gtggacctgc
     4921 tccatgcaga cggtcagatc aggctgccgc ctggcgagca tttcgcagat gcgtgcataa
     4981 cgtgttgggt tcataaaaca ttaatttcgg tttcgggtga ctttaatcac gtctggcatc
     5041 acgcggattt tgcgcatgat attcgccaga tgcacacggt cacgagcggt cagacgaata
     5101 aaggcgctgt agacgcgacc atctttctct tccgtattca aactttgaat attcgaggtc
     5161 gtggtgttaa ttgccgccgt caggtttgcc agcgcaccct gatgattgaa catctccacc
     5221 ttgatttcgg tgatgaactc ctgcgccgtc tctttatccc attccacagc cataaacttc
     5281 tctggctctt tctggtagcc acggatgtta cggcaggatt catggtggat caccagacct
     5341 ttaccggggc tgacgtgggc gataatcggg tcgccaggaa tggggcggca gcatttcgca
     5401 aaggtgatca gcacgccatc agcgcctttg atcggcagat gcccgtggct ttgagtcgcc
     5461 ggtggaatgg aggcgtcccc atgttgtaga tttttcgcga ccaccacgct cattgcgtta
     5521 ccaaggccga tttctgccag cagatcgtca agcgttgcca gcttcatgcg atccagctcg
     5581 cgctgaatat tttcctgcgg gatttcattg agcttacggc taccacccaa agcatggttg
     5641 agcagacgac ggcccaggct tacagaatca tcacgcttga ggtttttcag caactgacga
     5701 attttggcgc gcgctttcga gctaacgaca aagttcagcc aagcggcatt cgggcgagcg
     5761 cccggagcgg taatgatttc aacggtctga ccgctagtaa gcggctgcga cagcgggtaa
     5821 ggctggcggt cgacgcgtgc gcccacgcag gcatggccga tatcggtatg cactgcataa
     5881 gcgaagtcga cgggcgttgc accggcaggc agctcgacaa tgcgcccttc cggtgtgaaa
     5941 acgtaaatct catccgggaa gagatcggat ttaacgctct cgataaattc aaacgaacta
     6001 ccggcgctct gttgcagctc cagcaggctt tgcatccagc gctgggcgcg gatttgcgcg
     6061 gtggtgctgg tttcgccgtg ctctttataa gcccagtgcg cggcaacacc catctccgcc
     6121 atctgatcca tatcttcggt acggatctgg acctcaaccg gcacaccgtg cgggccgatc
     6181 atcgaggtgt gcaaagactg atagccgttc gcttttggaa tggcgatgta gtctttcacg
     6241 cgacccggac gcggcttgta caggctatgc atttgcccga gcacgcgata acaggtgtcg
     6301 gaatcattga cgatcacgcg gaaagcgtag atgtccatga ttgagtgaaa acgctgctct
     6361 ttgagcacca ttttgcagta aatcgaataa agatgcttct cgcgaccact gacgcggcac
     6421 ggaattcccg cttcctgcaa acgcccttcg atttcagaga ggattttttg gatcatctct
     6481 ttacggttgc cgcgcgcggc tttcaccact tctttaatta cgcggtaacg attgggatac
     6541 agcgcctcaa aacccagctc ttcgagttcg gttttaatgt ggtggatacc taaacggtgc
     6601 gccagcgggc tgtaaatttc gagagtttca cgggcgatgc ggcgacgttt gtccgggcga
     6661 agtgagccca gcgtgcgcat gttgtgggta cggtcggcaa gtttgatgag gatgacgcgg
     6721 atatcctgca ccattgccat aatcatcttg cgaaagtttt cggcctgcgc ctctttctta
     6781 tcacggaact tgagtttatc aagtttcgac accccctcta ccagctcggc gacgctttta
     6841 ccaaaaagct gttccatatc ctggtaggtg gcgggagtat cttcaatcac gtcatgcagc
     6901 agcgccgcca tcagcgtttc atagtcgagt ttcatctcgg ccagaatgca ggcaaccgct
     6961 accgggtgcg tgatataggg ttcaccgctt gaacgtgttt gcccctcgtg agcatcacgt
     7021 gcaacgagat acgcctgccg cagacgcttg atttggtctt ccggcaggta ggtttgaatc
     7081 agttgattca ggctttcaaa cagatacaag ggcgacccgc tttgtgatta acgacgacct
     7141 tcagcaatag cggtaacggc ttgtaattca gcggcttcct gctcttgctg ttcctggcgt
     7201 tcgcgaacgt cgaggatctg gttgttgatc agaccttctt cgatttcgcg cagcgcgatt
     7261 acagtggttt tatcgttttc ttccggtacc agcggatcct ttccgcctac ctgcatctga
     7321 cgagcgcgac gcgcggcgac cagtaccagg tcaaaacggt taccaatttt ctctacagcg
     7381 tcctgaacag ttacgcgtgc catacttaaa aagctccaca ggtgaagaaa tgactgggca
     7441 tgatactgaa atcaggttca gtctgccaac aatttgctga ttaaagcgtc atgacgctgc
     7501 ttttggcggc tcatgcgcag acgttcggcg cgaataatgg tcttcaaatc ggtcaacgcg
     7561 gtatcgaagt catcattcac aatcagataa tcatattcgg cgtaatggct catttctgca
     7621 acagcttgcg ccatacgctt tgcaatgacc tcttcgctgt cctgaccgcg accgcgtaga
     7681 cggcggtcca gttcaatttt ggacggcggt aaaataaaga tactccgcgc gtgcggcatc
     7741 ttctggcgaa tttgctgcgc gccctgccag tcgatatcga gaaaaacatc gacaccggtc
     7801 gccagtactt gctcaatggc ctcacgcgaa gtgccatagt aattaccaaa aacttctgcg
     7861 tgttcgagga acgcatctct gctaatcatt tctttaaatt catcatgatt aacaaagaaa
     7921 taatgttcac cgtggacttc accaggacgc ggttggcgtg tggtgtgtga aacagaaacc
     7981 tgggtgtcat acaacggttg ggtttttaat aaagcctgaa tcaggctgga tttacccgcg
     8041 ccactggggg cagaaacaat ataaagcgtg ccttgagcca tgagtatctt tcgtatgtga
     8101 ttagcgaaat aaagcctaca tacgagctta ttatacacgg cgcggccatg tgacgtagcc
     8161 cttgctcaca ctttttgcgc aaagttgttg cgttttgcgt agctgtttcc gcatttgcct
     8221 gtgatttact gcaataaaga cgattctcag gaagcgtcct cgcagactcc gtttttgcca
     8281 ctcggctttg ccttcaccac gttgtatatc tgccagcctg actacagcgg aggtgaagat
     8341 gaaagtatgg atggcgatat taataagtat cttgtgctgg caatcatctg tgtgggcggt
     8401 ctgtccggcc tggtcgccag ccagagcaca ggaagaaatt tcccgcctgc aacagcaaat
     8461 aaaacagtgg gacgatgact actggaagga aggaaaaagt gaggtggaag acggtgttta
     8521 cgatcagtta agcgcccgtc ttacgcagtg gcaacgctgc tttgtgagcg agccccgcga
     8581 cgtgatgatg ccgcctttaa atggtgcggt tatgcatccg gttgcccata cgggtgtacg
     8641 caaaatggcg gataaaaacg cattaagtct gtggatgcga gaacgtagcg atctttgggt
     8701 gcagccaaaa gttgatggcg ttgcggtaac cctggtttat cgggacggga aactgaacaa
     8761 agcaatcagt cgcggtaatg gcctgaaagg cgaggactgg acgcagaaag ttagcttaat
     8821 ttccgctgtg ccgcaaaccg ttagcgggcc tttagccaac agtacgcttc agggggaaat
     8881 atttctccag cgcgaggggc atatccaaca acaaatgggg ggaataaatg cccgcgcaaa
     8941 agttgctggc ctgatgatgc gccaggacga tagcgacacg ctgaattctc tgggcgtttt
     9001 tgtctgggca tggccggatg gaccgcagtt aatgaccgat cgtttaaagg agctggctac
     9061 cgcaggtttt actctaacgc agaggtatac ccgtgcggtt aaaaatgctg atgaggttgc
     9121 gcgcgtacgc aatgagtggt ggaaagcgaa attacccttc gtcaccgatg gcgtggttgt
     9181 gcgaggggcg aaagaacccg aatcccgcca ttggctacca ggccaggcag aatggctggt
     9241 ggcctggaaa tatcaacctg tagctcaggt tgccgaagtg aaggcgattc agtttgcggt
     9301 gggtaagagc ggtaaaatat cggtggttgc gtcactcgca cctgtcatgc tggatgataa
     9361 aaaagtccag cgggtgaata ttggttccgt caggcgctgg caggagtggg atattgcgcc
     9421 tggtgatcag atcctcgtca gccttgccgg tcagggtatt cctcgcattg atgatgtggt
     9481 ctggcgcggt gcagaacgta caaaaccgac accaccagaa aaccgcttta actcgttgac
     9541 ctgctacttt gcttctgatg tttgtcagga acagttcatt tcacgcttag tctggctggg
     9601 gtcaaaacag gttctggggc tggatggtat tggtgaggcc ggctggcgcg cgctgcatca
     9661 gactcatcgc tttgagcata tcttttcctg gcttttatta acgccagagc aattacagaa
     9721 cacgccgggg atcgcgaaaa gtaaaagtgc gcagctatgg catcggttta atctggctcg
     9781 taagcagcct tttactcgct gggtgatggc aatgggaata ccgctaaccc gggcggcgct
     9841 taatgccagt gatgaacggt cctggtcgca acttttattt agcacggagc agttctggca
     9901 gcagctgccg gggactggat cggggcgcgc cagacaggtt attgaatgga aggaaaatgc
     9961 gcaaatcaag aagctgggca gttggctggc tgcccagcag atcacaggtt ttgaacctta
    10021 gtgtccttcg tggctgtagt aaaagaccgg taatcccagc ttaaggcgta gcgccagtaa
    10081 gcgggcaaaa aagccgaata ccagggtgga gataattacc acatcatggt tagaaacata
    10141 gtgttgcagc gcgatgtaca gcacggcgga ggcaaatgag acaccggcat acaactcttt
    10201 ctggaatacc agtgggatac gtttacagaa catatcgcgc aaaacgccgc caaacacgcc
    10261 ggtggttacc gccgcgacaa cggcaataat tgggccgtgc cccatatcca gtgcaacctg
    10321 tgcgccgatg atagaaaaga ccaccagtcc aagcgcatcg agcaccagaa acactttgcg
    10381 caggtaaggc attacaggag caacgatagt ggtgagtact gcggcggtag cgacgataat
    10441 cacatattcc gggtgtttga cccagccgag cggatagtgg cccagcagaa tatcgcgcac
    10501 tgaccctccg ccaattgcgg tggcggtcgc aataataatt acgccaaatg tatccatgcg
    10561 gcgtcgcccg gccgccagag cccccgtcat ggcttcggca gtaatgccaa ccaaatacaa
    10621 aatgtgtaac agcatggttc tctccctaaa aatctgtctg gcagggtagc gatttgtgcg
    10681 tattgtcacg attgagattt tttaaggtga gaggaaattg ataacttgta gttatgtgaa
    10741 atcgctattt tctgtagcgt agatgcattc ttctgacttc ttgtctgaga aaatgcataa
    10801 gaaaaaatag gttaaaattg aggggtggaa aagaaaggtg caatgtttag ccattgtgtt
    10861 caccgcacct ggactttggc ttacgcatcg ggtatctcaa tcaacaaaaa gagtttttag
    10921 tttctgctat ttcttctctg taatttttac actgttttcc aactgcttga tgctgacctg
    10981 cggggtcggc aactcctcag gcatggttcc gccaagttcc tgaatggttt gcctcacctt
    11041 gctacccacg tcaaagtgtg tggtattagc ttgctgtttt gaattcacgc catcccgctt
    11101 gagtttttct tctgtttggg tagctcgaaa gagattagcc gccagttctg ttgaacccat
    11161 atgatccagg atcttctgac tctttttcag ccccttccgc tgatggatgg ctttctgatc
    11221 taatccacca tacagccccc ggtaaccatg attctgaaat atggcgaaat cgatagctgt
    11281 cgctacccca gcttgctgcg ctgcctcaac caattgtttg ttatgttctt tcaattcatt
    11341 tctcagaaaa agacgctttt cgtcttcacg aagttgcctg aaagcttcat cgtcagccag
    11401 ctcctgccgt cgggtctgga tagcaaaata agtttgccct gccgcaataa ccggtttcgc
    11461 agggtcgccg ttttgtacta ccaggtaaca ggcatagcgg gagagatgaa catcttcaag
    11521 ttctcgttga gcccctgaac ctaatacaac cattttggtg gtttcaacga aatggtcaga
    11581 tgcagcttga ttgctggctt cgcacgcttg agtcgcgcgg gccagtacct tttgaaaatt
    11641 gcgccagtcc cgataatcca gtaacggtgc caattctcta gctgaccaaa attcttgccc
    11701 ttcagtgcca taatgccgta tttcttcaaa aggttgatga tgttcgttca ttgcccactc
    11761 catgtgatat cgattgcaaa gaaagtaaat ctgtaactgt atttatatac agttgtgagt
    11821 atctcacgtt ctcagtgtgc tgggcaaaaa aacatcgcaa aatctggagc aggcacgcaa
    11881 aacagaattg aagttgcggg gaagaaaaat gccgctctgt ttgagtgggc ggcatttgtg
    11941 aaggctgaaa agtaagaatt agtgttacga gttactcaat attctgaatc tgctcgcgca
    12001 tctgctcaat caacactttc agctcaatcg cggagtttgt cacttcggca ttgatagact
    12061 tcgacgcaag agtgttcgac tcgcggttga attcctgcat cataaagtcc agacgacgac
    12121 caacggcttc ttttttcttc agaatgttgt aggtctcttt gacatgcgct tccaggcggt
    12181 ccagttcttc ggcaacgtca attcgttgtg ccagcagaac caactcctgc tccaggcggt
    12241 tgttttccag ttgcacttga gcatcttcca gcttcgcgac cagacgctca cgctgccatt
    12301 gcaggatttc cggcatatgg gcgcggactt tgaccacttc ggcggtgacg ccttccagac
    12361 gctgctcgat caatgctttc agcgcctgac cttcggtttc acgtgcgacg ataaagtcgt
    12421 ccagggtacc atccaacgcc gcgagaattt cagcggcaat cgcgtcgaga tcctgctcct
    12481 gggctgccat tacgcccggc cagcgcagaa tatcaaccgg gttgatttcc ccttcgtcac
    12541 tctgcatttt tacccagttc gcggcagtta ccagctgttt agccagtttt tcgttgagga
    12601 tcagctcacc ttgcgcgcta acatctggct catagcgcag ggtacattcc actttaccgc
    12661 gcgtcaggcg agaacgaata cgctcgcgaa cgacaggttc aaggctacgg aactgctccg
    12721 gcagacgaaa gtaagtttcc agataacgct ggtttaccga gcgcatttcc caggttgcgc
    12781 tcccccattc acccttgatt tcacgccggg cgtaggcggt catactgcgg atcatagacg
    12841 ttcctgttta taaaaggaga ggtggaagga ttatagccat cgatgccttg tcaggatagg
    12901 aataaccgcc ggaagtccgt ataatgcgca gccacatttg tttcaagccg gagatttcaa
    12961 tatgcgtcca gcaggccgta gcaataatca ggtgcgtccc gttaccctga ctcgtaacta
    13021 tacaaaacat gcagaaggct cggtgctggt cgaatttggc gataccaaag tgttgtgtac
    13081 cgcctctatt gaagaaggcg tgccgcgctt cctgaaaggt cagggccagg gctggatcac
    13141 cgcagagtac ggcatgctgc cacgttctac ccacacccgt aacgctcgtg aagcggcgaa
    13201 aggtaagcag ggtggacgca caatggaaat ccagcgtctg atcgcccgtg ctcttcgcgc
    13261 ggcagtagat ttgaaagcgc tgggtgagtt caccattacg ctggactgcg acgtgcttca
    13321 ggctgatggt ggcacgcgta ccgcgtcgat tacgggtgcc tgcgtggcgc tggcagatgc
    13381 gctacagaag ctggtggaaa acggcaagct gaaaaccaac ccgatgaaag ggatggtagc
    13441 cgcagtttct gtcggtattg taaacggtga agcaatttgc gatctggaat acgttgaaga
    13501 ctcagccgca gagaccgaca tgaacgtagt gatgaccgaa gacgggcgca tcattgaagt
    13561 gcaggggacg gcagaaggcg agccgttcac ccatgaagag ctactcacct tgttggcgct
    13621 ggcccgaggg ggaatcgaat ccattgtagc gacgcagaag gcggcgctgg caaactgatt
    13681 tttaaggcga ctgatgagtc gccttttttt tgtctgtaga aaagtaagat gaggagcgaa
    13741 ggcatgaaac catatcagcg ccagtttatt gaatttgcgc ttggcaagca ggtgttaaag
    13801 tttggcgagt ttacgttgaa gtccgggcgc aaaagcccct atttcttcaa cgccgggctg
    13861 tttaataccg ggcgcgatct ggcactgtta ggccgttttt acgctgaagc gttggtggat
    13921 tccggccttg agttcgatct gctgtttggc cctgcttaca aagggatccc gattgccacc
    13981 actaccgccg tggcgctggc ggagcatcat gaccttgacc tgccgtactg ctttaaccgc
    14041 aaagaggcaa aagaccacgg tgaaggcggc aatctggttg gtagcgcgtt acaaggacgc
    14101 gtaatgctgg tagatgatgt gatcaccgcc ggaacggcga ttcgcgaatc gatggagatt
    14161 attcaggcta atggcgcgac gcttgctggc gtgttgattt cgcttgatcg tcaggaacgg
    14221 gggcgcggcg agatttcggc gattcaggaa gttgagcgtg attacaactg caaagtgatc
    14281 tctatcatca ccctgaaaga cctgattgct tacctggaag agaagccgga aatggcggaa
    14341 catctggcgg cggttaaggc ctatcgcgaa gagtttggcg tttaaagaaa ctcgccggat
    14401 gaaaagtcat ccggcgtcat attactgcaa ctgtgccgca attagcggcc agcgggcgtc
    14461 aaaatcatcc gtcgggcggt atttaaattc gctgcggaca aaacgtgaca gcataccttc
    14521 acagaaggcc aggatctggc ttgccagcag ggtttcatcg gtggtgtaac cttcaccctc
    14581 acgcattctc ttttcacgca atacctggcg cagctgcgct tcaatacgct cgaacagctg
    14641 gttgatgcgc ccttgcaggc gatcctgttc aaacattagc gcatgaccag tgaggatgcg
    14701 ggtcaggcca ggattacgct caccaaaacc gagaagcagc aacacaatca gacgcaggcg
    14761 cgctgtggtg tctttctcat ctttcagaat caggttgatg cgagtaatca ggctatcttc
    14821 gataaactca atcaggctat cgaacatgcg ggtcttactg gggaagtggc gatacagtgc
    14881 cgcttcggaa acgccgacag aggcggccag ttttgccgtc gtgatacgtt ggcttccatc
    14941 gctggattcc agcatcagcg ccagagactg aagtatttcc tcgcgacggt tccttttcgc
    15001 agtttgtttt tctgccatgt tacaaaatac ccctgaaaat aagcacttgc caggcggcac
    15061 ccacgctatg accgcaaacg aaatgtttgc ggctatgtta tgacgttatt cggatgcgta
    15121 tgtgttactg acgaccagag tgaccaaagc cgccttcacc gcggtcggtg gcgtcgaaat
    15181 cttccaccag attaaattca gcctgtacta ccggaacaaa aatcatctgg gcgatgcgtt
    15241 cgccaggttg aatggtgaag ctgtcctgac cacggttcca cacggaaatc atcaactggc
    15301 cctgatagtc agaatcgatc aatcctacca ggttaccaag cacgataccg tgcttatgtc
    15361 ccaatccgga gcgcggcagc atcattgccg ccagtgaagg atcggcaata tgaatcgcca
    15421 gcccggtcgg aaccagcgta gtgtcacccg gagccagttc tacggcgtcg tcgagacagg
    15481 cacgcaggtc aagtccggca gagccagagg tggcataagt cgggagcgga aattccttcc
    15541 caacgcgcgg gtccagaatc ttaacgtcga tttttttcat cataacgggt cacgatctcg
    15601 tcgagtaata attggccaag gagctctttg cgctcaagcg gtaagacttt atctccgtcc
    15661 tgccagaaaa ggtgtaatgc gttgttgtcg ctgttaaatc cttgagttgg ctgggaaaca
    15721 tcgttcgcgc agatcagatc aaggttttta cggatacgtt tttgccgggc gtattcttcc
    15781 acattatttg tttcggcggc aaatccaacg acgtagggtc gatggtcttt tagtgcggca
    15841 acgcctgcga cgatatcggg gtttttaacc atttttattg ttaattcatc accctgcgtg
    15901 gcctgctttt tgattttctc tggggccacg gtagctgcgc gataatccgc cacggcggcg
    15961 cagccgataa aaatattttg ctgctgtaca gaagcattca cggcggcttc catttccagc
    16021 gcagtcatca catcaacacg tttaacaaac ggtggcgtcg gtagtgaaac cggacctgat
    16081 accagcgtga cgttcgcgcc acgacgggca gcggcggcgg cgatagcaaa acccatcttg
    16141 ccggagctgt gattagagat ataacgcacc ggatcgagcg gttcacgcgt cggaccggcg
    16201 gtaatcataa tgttcagatg tttcaggtcg ttgacgggcg aaaaatgcgc caccgccata
    16261 tccacaatgg ttaacggatc gagcattcgc ccaggaccga tatcaccaca agcctgactg
    16321 ccactgtctg gcccccagat gagcaaacca cgggaagcaa gcacctctaa attatgctgc
    16381 gtggcagcgg cacggtacat ctgctggttc atggcgggga gcacggctac aggcgcaggt
    16441 gtagccagac aaatcgtcga taccaggtca ttcgccattc cggcagcaac acgggcaatc
    16501 aaatctgccg tggcaggggc gagaatcact aaatcagccc atttacccag ctcaatatgg
    16561 cccatagcgg cttcggctgc cgggtccagc aaactgtcgg aaacgggata accagaaacc
    16621 gcctgcaagc taagtggggt gataaaggct tttgccgctt cggtcatggc tacgcggacg
    16681 tcggccccgc gatcgcgcaa acgacgcacc agttcagggg ttttataggc agcaataccg
    16741 ccgctaacgc cgagaacgat ttttttaccg gccaggctca tgatgatttt tcctgttggg
    16801 tgacaccaga agttggcgat tttatcacaa tacttttgtt gtcgtgcctt cacctgagaa
    16861 tcactttgcg aggcgctttc caggattgaa aactggccgt cgatttaacg gaacggctat
    16921 gacaggatgc gagcaccaca aaggaggtga aggtgaaaaa caatgcacag ctgttgatgc
    16981 cgcgcgaaaa aatgctgaag tttggtatta gcgccttaac ggatgtcgag ctgctggcgt
    17041 tatttctgcg taccggaacg cgcggtaaag atgtattaac cctggcaaaa gagatgctgg
    17101 agaatttcgg ctctctttat ggcttgttaa cctctgaata tgaacaattt agtggcgttc
    17161 atggaattgg cgtagcgaaa tttgcccagt taaaggggat tgctgaactg gcgcggcgtt
    17221 actacaacgt gcggatgcgt gaagaaagcc ctttactcag cccggagatg acgcgggaat
    17281 ttttacaaag ccagctcacg ggtgaggagc gggagatctt tatggtgatc tttctcgact
    17341 cacaacaccg ggttataacg catagccgtc ttttttccgg cacgctaaac catgttgaag
    17401 tccatcctcg ggaaattatc cgcgaagcga taaaaataaa cgcctcggcg ctgatccttg
    17461 cacataatca cccttcgggt tgtgctgaac ccagtaaagc ggataaactc attactgaac
    17521 ggataataaa gagttgtcag ttcatggatt tacgcgtgct cgaccatatc gtgattgggc
    17581 gtggagagta tgtttctttt gccgaacgcg gctggattta acccgctatg cgcgatcctt
    17641 cgggatcttt gtctgttcgg gacttgagca catcgctgag tcagcgtata ctacgccacc
    17701 tttgagaatc tcgggtttgg catttgggcc tggcaatcga gagttcacat agaactgcga
    17761 tgaccgggct gtaaagcctg acgaggcgcc aataccccat acgaagctcg agctaatttg
    17821 atttttggag aatagacatg tcccgagtct gccaagttac tggcaagcgt ccggtgaccg
    17881 gtaacaaccg ttcccacgca ctgaacgcga ctaaacgccg tttcctgccg aacctgcact
    17941 ctcaccgttt ctgggttgag agcgagaagc gttttgtcac cctgcgcgta tctgctaaag
    18001 gtatgcgtgt aatcgataaa aaaggcatcg atacagttct ggctgaactg cgtgcccgtg
    18061 gcgaaaagta ctaagtactt agaggaaata aatcatggct aaaggtattc gtgagaaaat
    18121 caagctggtt tcttctgctg gtactggtca cttctatacc actacgaaga acaaacgtac
    18181 taagccggaa aaactggaac tgaaaaaatt cgatccagtt gttcgtcagc acgtgatcta
    18241 caaagaagcg aaaatcaaat aattctcgct ttgatgtaac aaaaaacctc gctccggcgg
    18301 ggttttttgt tatctgcttg cccccatatt gattgcatct gttcattcct ggagatgcta
    18361 tgcctgaatt acccgaagtt gaaaccagcc gtcgcggcat agaaccgcat ctcgttggtg
    18421 caaccattct tcatgcagtg gtgcgcaacg gacgcttgcg ctggccggtt tcagaagaga
    18481 tctaccgttt aagcgaccaa ccagtgctta gcgtgcagcg gcgggctaaa tatttgctgc
    18541 tggagctgcc tgagggctgg attatcattc atttggggat gtctggcagc ctgcgcatcc
    18601 ttccagaaga acttccccct gaaaagcatg accatgtgga tttggtgatg agcaacggta
    18661 aagtgctgcg ctacaccgat ccgcgccgct ttggtgcctg gctgtggacc aaagagctgg
    18721 aagggcataa tgtgctggcc catcttggac cggagccgct cagcgacgat ttcaatggtg
    18781 agtatctgca tcagaagtgc gcgaagaaaa aaacggcgat taaaccgtgg ctgatggata
    18841 acaagctggt ggtaggggta gggaatatct atgccagcga atcactgttt gcggcgggga
    18901 tccatccgga tcggctggcg tcatcactgt cgctggcaga gtgtgaattg ttagctcggg
    18961 tgattaaagc ggtgttgctg cgttcgattg agcagggcgg tacaacgctg aaagattttc
    19021 tgcaaagtga tggtaaaccg ggctatttcg cacaggaatt gcaggtttac gggcgaaaag
    19081 gcgagccgtg tcgggtgtgc ggtacgccga ttgtggcgac taaacatgcg cagcgggcaa
    19141 cgttttattg tcggcagtgc cagaagtaat tcatgcgcgc cggatggcat accatccggc
    19201 ataaacgcta cgctaacttc gccatcagcg cctgatggac attctccggc aggaaatggg
    19261 tgacatcgcc ctgatggcgc gccacctctt tcaccaacga tgaagagata aacgaccact
    19321 ctttcgacgg catcagaaac acactttcca gttccggcat taagtggcga ttcatatgcg
    19381 ccagctgcat ttcatattca aaatccgcca ccgcacgcag gccacgaatc agcaccgtag
    19441 cgtgttgatt acgggcgaag ttcgccatca aatcactaaa cccgaccact tccacgttcc
    19501 ccagatgcgc ggttgcctgc tgtgccagcg ccacacgctc ttccagggta aacatcggtt
    19561 ttttactggg gctggcggca atcgccagaa taacgtgatc gaacatttgc gtggcgcgcg
    19621 tcacgatatc gatatgacca ttggtaatgg gatcgaaagt acccggataa atcgcccgtt
    19681 tttgcataac aacctcaatg cgttttcggt ggcaggtaag gttccagcag ttgaagcaga
    19741 cgctgtagcg cgccctggtt ttgatacagt acttcaacgg catgacggcc atagaaacta
    19801 cggtaatcgg cgtcggtgag taaagaggac acctcttttg caagcgtagt ggcatcggta
    19861 acggtaatca gcccgcttgc ctgctccagc cgcgcgcaaa tgtctttaaa gttaaaagta
    19921 tgcggcccca tcaataccgg aatagcgtgt gcggcagctt ccagcggatt atgcccacca
    19981 cgttcaacca gtgaaccgcc aacaaaggcg agatcggcaa tgccgtacag taacatcaac
    20041 tcgcccatcg tatcgccaac cacaacctgc gtgctggtgg aggggacttc ccctgaagag
    20101 cgtgtgatat agcttagtcc agcctggcgg acaagattaa tcgcatccgg gaagcgttct
    20161 ggatgacggg gtaccaggat gagcaataaa ttcgggaatt gctgtaacaa tgcctgatgt
    20221 gcggcgatca ccacactctc ttcgccttcg tgagtgctgg tggcaatcca taccgggcgg
    20281 tgtggtgccc actggcggcg cagcgtcacg gctttagcag ccaactgcgg cgttacagaa
    20341 atatcgaatt tcaggctacc ggtaacggtc acctgattat tttttgcgcc cagcgccaca
    20401 aaacgtgcac catcttcttc attttgcgca gcaatcagcg taatacgacg cagcaagcga
    20461 cggacgaatt tacccagttt ggcataacct gcggccgagc gggcagagag tcgcgcgtta
    20521 gcgatcacca gcggaatttt acgtttgtgt agcgccgcaa tcaggttagg ccatagttcg
    20581 gtttccataa tcaacaccag tttggggtcg actttattca ggaaacggtt gagcgcatcg
    20641 ggcaggtcat acggcagata aacgtgctga acatccttcc cgaaagccga ttgtacgcgc
    20701 tccgaaccgg ttggcgtcat ggttgtaacg gtaatcggta aatcaggata acgatggcgc
    20761 agcgcgcgca ccaacgggat cgccgccaga gtttcaccga cggagacgga gtgcagcata
    20821 atgccgcctg gttttagcgg atggcggtaa aaaccgtaac gttcacccca gcgttttcga
    20881 taggccggag ccttacgtcc gcgcacccag agccgtatcc agatcagcgg ctgaataagg
    20941 tagagaaggg cggtgtaaag caattcgagc atagtaaata gctgacttat ggatgtgctg
    21001 gggattctat gtatttagct gtggctttac cattactttt cccgtttttg acttaaatag
    21061 caatttaccc gccaattctt gctgaacatc tatacttttt ttgtattttt atgcgattca
    21121 ttgatttctg ctgtacgtgc aggaaggaat cgcagagcaa cagtgaaaga atatggttgc
    21181 taatgatttt atttgattga tttctttatt gaaaatcaac aatgtgctta aaaatcattc
    21241 gggtcggatt gccgcgaaag aagtgataca ctagcgcgtc aatatcagtg cggtatcagt
    21301 attcaggtag ctgttgagcc tggggcggta gcgtgctttt tctgcttcac cttaatcgga
    21361 taatctcaac aaaaagagtt acttgtggat aagccatttc gaagaatttt gctcattaag
    21421 atgcgttttc atggggatat gttattaact actcccgtca ttagttcgct gaaaaaaaat
    21481 taccctgacg caaaaatcga tgtgctgctt tatcaggaca ccatcccgat cctgtctgaa
    21541 aatccagaga ttaacgcgct ctacggcata aaaaataaaa aagcaaaagc ctcagaaaaa
    21601 attgccaact tttttcatct catcaaggta ttacgtgcca ataagtatga ccttatcgtc
    21661 aatctcaccg atcaatggat ggttgctata ctggttcgct tattaaatgc ccgtgtgaaa
    21721 atttcccagg attatcatca tcggcagtct gctttttggc gtaaaagttt cacccatttg
    21781 gtgccgttgc agggtggaaa tgtggtggaa agtaacttat ccgtgctgac cccattggga
    21841 gttgattcgt tggtgaagca gacaaccatg agttacccgc ctgcaagctg gaaacgtatg
    21901 cgtcgcgaac ttgatcacgc tggtgttgga caaaattatg tggttatcca acctacggcg
    21961 cggcaaatct tcaaatgctg ggacaacgcc aagttttccg ctgtgattga tgccttacat
    22021 gctcgtggtt atgaagtcgt tctgacgtcc ggcccagata aagacgatct ggcctgcgtc
    22081 aatgaaattg cgcagggatg ccagacgcca ccagtaacgg cgctggctgg aaaggtgacc
    22141 ttcccggaac ttggtgcgtt aatcgatcat gcgcagctgt ttattggcgt tgattccgca
    22201 ccggcgcata ttgccgctgc agttaatacg ccgctgatat cgctgtttgg tgcgacagat
    22261 catattttct ggcgtccctg gtcaaataac atgattcaat tctgggcggg agattaccgg
    22321 gaaatgccaa cgcgcgatca gcgtgaccga aatgagatgt atctttcggt tattccggcg
    22381 gcagatgtca ttgctgctgt cgataaatta ctgccctcct ccacgacagg tacgtcgtta
    22441 tgatcgttgc tttttgttta tataaatatt ttccctttgg cggtttgcag cgtgatttta
    22501 tgcgtattgc tcagacagtc gccgcccgag gtcatcatgt tcgggtttat acccagtcgt
    22561 gggaaggcga atgccctgat gtatttgaac tgatcaaagt gccggttaaa tcgcatacca
    22621 atcacgggcg caatgcggag tattttgcct gggtgcaaaa acatttacgc gaacatcccg
    22681 tcgataaagt cgttggattc aacaaaatgc cggggctgga cgtttattat gccgctgatg
    22741 tttgttatgc cgagaaagta gcgcaggaaa aaggcttttt ctatcgcctg acgtcacgtt
    22801 atcgccatta tgccgccttt gagcgggcaa ccttcgaaca gggtaagccg acacagctgc
    22861 tgatgctgac agataagcaa atcgccgatt tccagaaaca ttatcagact gaagcggagc
    22921 gttttcatat tctgccaccg gggatttatc ctgatcgtaa atatagccag cagccagcca
    22981 atagccgtga aatcttccgt aagaagaatg gaataaccga acaacaatat ttattgttgc
    23041 aggtcggttc agacttcacg cgtaaaggtg tcgatcgttc cattgaagca cttgcttcgt
    23101 taccggattc gctgcgccac aacacattgc tatatgttgt tgggcaggat aaaccgcgaa
    23161 aatttgaggc gctggcagaa aaacgcggcg tgtgcagtaa tgttcacttt ttctcggggc
    23221 gcaatgatgt ctcggaatta atggcggcgg cggatttatt actgcatcct gcctaccagg
    23281 aagcggcggg aattgtgctg ctggaagcga ttactgcagg attaccggta ctaacaacag
    23341 ccgtttgtgg ctatgcgcct tatattgtcg aggctaattg cggcgaggct attgctgagc
    23401 cattccgcca ggaaacattg aatgagattt tacgcaaagc gttaacgcaa tcttcattgc
    23461 gccaggcctg ggcggaaaat gcgcgatatt atgctgatac acaagattta tacagtctgc
    23521 cagagaaagc ggcggatatc ataacgggtg gtctggatgg ttgaacttaa agagccgttt
    23581 gccacgttat ggcgcggcaa agatcctttt gaggaagtta aaaccttgca gggtgaggta
    23641 tttcgtgaac tggaaactcg ccgtactctg cgctttgaaa tggcgggcaa aagctatttt
    23701 ctcaaatggc atcgcggcac gaccctgaaa gagataatca aaaatttact ctcattgcgg
    23761 atgccagtat taggcgcaga ccgcgaatgg aatgcgattc atcgactgcg ggatgtcggc
    23821 gttgatacta tgcatggggt ggcatttggc gaaaaaggca tgaatccgct gaccagaact
    23881 tcatttatta ttaccgaaga tctgacacca accataagtc tggaagatta ctgtgctgac
    23941 tgggcgacta accctccaga tgttcgcgta aagcgtatgc ttattaagcg tgtcgcgacg
    24001 atggtgcgcg atatgcatgc tgcgggcatt aaccaccgtg actgttatat ctgtcatttc
    24061 ctgctgcact tgcctttttc cggtaaggaa gaggagttaa aaatttcggt aattgacctg
    24121 caccgggcgc agcttcgcac gcgcgttcca cgtcgttggc gggataaaga tcttattggg
    24181 ctttattttt cttcgatgaa tatcggcctg actcagcggg atatctggcg gtttatgaaa
    24241 gtgtattttg ccgccccgct taaagacatt ctcaagcagg aacaaggact gctgtcgcaa
    24301 gcagaagcaa aagccacaaa aatcagggaa agaacgattc gaaaatcgtt gtaacccgta
    24361 acttattttt gccaaaattt tggatacaga ataaatatgt ctcaactcaa tgatagtgac
    24421 atcatccttt ttgagtataa ttttcattat caaaatataa gatctaaaaa tactcttgat
    24481 atagcatttg gtattgacag aaattttctt tttggatgtg gtgtagccat cgcatctatt
    24541 ctattaaaca atagagaaat ctcttgtgaa tttcatgttt tcacagatta tatcagtgat
    24601 aaagacaaat tatatttt
//
LOCUS       DAZGLJ010000051        24384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000051 DAZGLJ010000000
VERSION     DAZGLJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.051"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..363
                     /locus_tag="ACHK49_001846"
     CDS             <1..363
                     /locus_tag="ACHK49_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO9262505.1"
                     /translation="YQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGY
                     TLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYL
                     SDHPKLQGIAQQNSFKHT"
     gene            519..2054
                     /gene="gadC"
                     /locus_tag="ACHK49_001847"
     CDS             519..2054
                     /gene="gadC"
                     /locus_tag="ACHK49_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO9262506.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2185..3504
                     /locus_tag="ACHK49_001848"
     CDS             2185..3504
                     /locus_tag="ACHK49_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO9262507.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLPQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVQELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3881..5263
                     /gene="dosC"
                     /locus_tag="ACHK49_001849"
     CDS             3881..5263
                     /gene="dosC"
                     /locus_tag="ACHK49_001849"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO9262508.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5288..7687
                     /gene="dosP"
                     /locus_tag="ACHK49_001850"
                     /gene_synonym="pdeO"
     CDS             5288..7687
                     /gene="dosP"
                     /locus_tag="ACHK49_001850"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO9262509.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7945..8526
                     /gene="ddpX"
                     /locus_tag="ACHK49_001851"
     CDS             7945..8526
                     /gene="ddpX"
                     /locus_tag="ACHK49_001851"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO9262510.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8540..10090
                     /locus_tag="ACHK49_001852"
     CDS             8540..10090
                     /locus_tag="ACHK49_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO9262511.1"
                     /translation="MKRSISFRPTLLALVLATNFPVSHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10092..11114
                     /locus_tag="ACHK49_001853"
     CDS             10092..11114
                     /locus_tag="ACHK49_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9262512.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11111..12007
                     /gene="ddpC"
                     /locus_tag="ACHK49_001854"
     CDS             11111..12007
                     /gene="ddpC"
                     /locus_tag="ACHK49_001854"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO9262513.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LALAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12004..12990
                     /locus_tag="ACHK49_001855"
     CDS             12004..12990
                     /locus_tag="ACHK49_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9262514.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTLRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            12983..13909
                     /locus_tag="ACHK49_001856"
     CDS             12983..13909
                     /locus_tag="ACHK49_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO9262515.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTVPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(13965..14396)
                     /gene="osmC"
                     /locus_tag="ACHK49_001857"
     CDS             complement(13965..14396)
                     /gene="osmC"
                     /locus_tag="ACHK49_001857"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO9262516.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            14742..14957
                     /gene="bdm"
                     /locus_tag="ACHK49_001858"
     CDS             14742..14957
                     /gene="bdm"
                     /locus_tag="ACHK49_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO9262517.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15059..15196
                     /gene="sra"
                     /locus_tag="ACHK49_001859"
     CDS             15059..15196
                     /gene="sra"
                     /locus_tag="ACHK49_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO9262518.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15353..17050
                     /gene="maeA"
                     /locus_tag="ACHK49_001860"
     CDS             15353..17050
                     /gene="maeA"
                     /locus_tag="ACHK49_001860"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO9262519.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17184..18194
                     /gene="adhP"
                     /locus_tag="ACHK49_001861"
     CDS             17184..18194
                     /gene="adhP"
                     /locus_tag="ACHK49_001861"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO9262520.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            18341..18625
                     /locus_tag="ACHK49_001862"
     CDS             18341..18625
                     /locus_tag="ACHK49_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO9262521.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(18676..19329)
                     /gene="fdnI"
                     /locus_tag="ACHK49_001863"
     CDS             complement(18676..19329)
                     /gene="fdnI"
                     /locus_tag="ACHK49_001863"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO9262522.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19322..20206)
                     /gene="fdnH"
                     /locus_tag="ACHK49_001864"
     CDS             complement(19322..20206)
                     /gene="fdnH"
                     /locus_tag="ACHK49_001864"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO9262523.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20219..23266)
                     /gene="fdnG"
                     /locus_tag="ACHK49_001865"
     CDS             complement(20219..23266)
                     /gene="fdnG"
                     /locus_tag="ACHK49_001865"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(22679..22681),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO9262524.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENDKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            23498..24379
                     /gene="yddG"
                     /locus_tag="ACHK49_001866"
     CDS             23498..24379
                     /gene="yddG"
                     /locus_tag="ACHK49_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO9262525.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         5957 a         6188 c         6178 g         6061 t
ORIGIN      
        1 taccaggttg ccgcttatct ggcggatgaa atcgccaaac tggggccgta tgagttcatc
       61 tgtacgggtc gcccggacga aggcatcccg gcggtttgct tcaaactgaa agatggtgaa
      121 gatccgggat acaccctgta cgacctctct gaacgtctgc gtctgcgcgg ctggcaggtt
      181 ccggccttca ctctcggcgg tgaagccacc gacatcgtgg tgatgcgcat tatgtgtcgt
      241 cgcggcttcg aaatggactt tgctgaactg ttgctggaag actacaaagc ctccctgaaa
      301 tatctcagcg atcacccgaa actgcagggt attgcccagc agaacagctt taaacatacc
      361 tgataacgtt taacggtaac ggtgtcccga aacgaacccg tttcgggaca atttccaaag
      421 tctgttcact ggcattagca acggaaaata ttgttctgaa tacgcttcag aacaaaacag
      481 gtgcggttcc gacaggaata ccgttttagg gggataatat ggctacatca gtacagacag
      541 gtaaagctaa gcagctcaca ttactcggat tctttgccat aacggcatcg atggtaatgg
      601 ctgtttatga ataccctacc ttcgcaacat cgggcttttc attagtcttc ttcctgctat
      661 taggcgggat tttatggttt attcccgtgg gactttgtgc tgcggaaatg gccaccgtcg
      721 acggctggga agaaggtggt gtcttcgcct gggtatcaaa tactctgggg ccgagatggg
      781 gatttgcagc gatctcattt ggctatctgc aaatcgccat tggttttatt ccgatgctct
      841 atttcgtgtt aggggcactc tcctacatcc tgaaatggcc agcgctaaat gaagacccca
      901 ttaccaaaac tattgcagca ctcatcattc tttgggcgct ggcattaacg cagtttggtg
      961 gcacgaaata cacggcgcga attgctaaag ttggcttctt cgccggtatc ctgttacctg
     1021 catttatttt gatcgcatta gcggctattt atctgcactc cggtgccccc gttgctatcg
     1081 aaatggattc gaagaccttc ttccctgact tctctaaagt gggcaccctg gttgtatttg
     1141 ttgccttcat tttgagttat atgggcgtag aagcttccgc aacccacgtc aatgaaatga
     1201 gtaacccagg gcgcgactat ccactggcta tgttactgct gatggtggcg gcaatctgct
     1261 taagctctgt tggtggtttg tctattgcga tggtcattcc gggtaatgaa atcaacctct
     1321 ccgcaggggt aatgcaaacc tttaccgttc tgatgtccca tgtggcaccg gaaattgagt
     1381 ggacggttcg cgtgatctcc gcactgctgt tgctgggtgt tctggcggaa atcgcctcct
     1441 ggattgttgg tccttctcgc gggatgtatg tcacagcgca gaaaaacctg ctgccagcgg
     1501 cattcgctaa aatgaacaaa aatggcgtac cggtaacgct ggtcatttcg cagctggtga
     1561 ttacttctat cgcgttgatc atcctcacca ataccggtgg cggtaacaac atgtccttcc
     1621 tgatcgctct ggcgctgacg gtggtgattt atctgtgtgc ttatttcatg ctgtttattg
     1681 gctacattgt gttggttctt aaacatcctg acttaaaacg cacatttaat atccctggtg
     1741 gtaaaggggt gaaactggtc gtggcaattg tcggtctgct gacttcaatt atggcgttta
     1801 ttgtttcctt cctgccgccg gataacatcc agggtgattc taccgatatg tatgttgaat
     1861 tactggttgt tagtttcctg gtggtacttg ccctgccctt tattctctat gctgttcatg
     1921 atcgtaaagg caaagcgaat accggcgtca ctctggagcc aatcaacagt cagaacgcac
     1981 caaaaggtca cttcttcctg cacccgcgtg cacgttcacc acactatatt gtgatgaatg
     2041 acaagaaaca ctaaccatgt ctgaatggtt ataagacaag ggagcgataa gtcatcgctc
     2101 cctttttcgt gcttgctgtc gtattgactt aaccgggata agtacgagaa tgagcgcaca
     2161 tctgtttacc ggaaaccagc acatatggat atctgctccc gaaacaagaa attaacgatt
     2221 agaagaccag cgatactagt tgcactggca cttttactgt gtagttgtaa aagcacgcct
     2281 ccagagtcca tggtgacacc accagcaggt tcaaagccac cagccacgac gcaacaatcg
     2341 tcacaaccga tgcgtggcat ctggctggcc acggtttctc ggctcgactg gccaccggtt
     2401 tcctcggtta acattagtaa ccccaccagc cgggcccgtg tacaacaaca ggcgatgatc
     2461 gacaaactgg atcatctgca acgtctcggc ataaacacgg tctttttcca ggtcaagccg
     2521 gacggtaccg ccctgtggcc atcgaaaatt ttgccgtggt ccgatcttat gaccggtaag
     2581 attggtgaaa atccgggtta cgatccgctg caatttatgc tcgacgaagc ccacaagcgt
     2641 gggatgaaag tacacgcctg gtttaacccc tatcgcgtat cggttaatac gaagcccggt
     2701 actatcaggg aactgaatag cactctgcct caacaaccgg cgagcgtcta tgtgcaacac
     2761 cgcgactgga tcagaacgtc tggcgatcgc tttgtcctcg acccgggcat ccctgaggtt
     2821 caggactgga tcacatcaat agtcgcagaa gtggtttccc gctatcccgt agatggcgtg
     2881 cagtttgacg actatttcta tacggagtca ccgggttcac ggctaaatga taacgaaacg
     2941 taccgtaaat acggaggcgc atttgcgtca aaagcagact ggcggcgcaa caatactcag
     3001 cagttaattg caaaggtatc gcacaccatt aaaagcatta agccgggagt cgaatttggt
     3061 gttagcccgg caggcgtgtg gcgtaaccga tcacacgatc cgctcggttc cgatacccga
     3121 ggcgcggcag cctatgacga atcctacgct gacacccgtc gatgggtgga acaaggattg
     3181 ctggattaca ttgctcccca aatttactgg ccgttctcac ggagtgccgc gcgttatgac
     3241 gtgttggcaa aatggtgggc ggatgtcgtt aaaccgacca ggacccgcct gtatatcggt
     3301 atcgccttct ataaagtggg tgaaccttca aagatagagc cagactggat gattaacggc
     3361 ggcgtacagg aactgaaaaa gcagctcgat cttaacgatg cggtaccaga aattagcggc
     3421 accatcttgt tccgtgagga ctatctgaat aaaccacaga ctcaacaagc ggtcagctat
     3481 ctgcaaagtc gttggggcag ttaaactccc attcttactt tgtaacaagc cggtgcttta
     3541 cccaccggct tgttaccctc ttgaactatc ccactactaa tcatgcttac taaagtcaaa
     3601 ttaaccacgc aattaaccac acttagttaa acagccataa atcccctaat aaaaatgtat
     3661 gaatatgaaa aaacatacga tattcgctgt caccgaaaaa taattacgat gggctatact
     3721 cttctgaaga acagtagcgt agattaaaac tgtcctaagt cgcttatttc ttttcagtat
     3781 atcttcatat ttcaggagaa tatggtgcag ttagtcgatc tcgcacgttg cgtttcgttt
     3841 tgattccgtc tgatttccca gccttataag ggtgtgaatt atggagatgt attttaaaag
     3901 aatgaaagat gagtggaccg gacttgtcga acaggcagat cctctcattc gtgctaaagc
     3961 cgcagaaatt gccgttgcgc atgctcatta tctgagtatc gagttttatc gaattgtccg
     4021 catcgacccg catgccgaag aattcttgag taatgaacaa gttgagcggc agttgaaaag
     4081 tgcgatggaa cgctggatta ttaacgtgct ttctgcccag gttgacgatg tcgaaaggct
     4141 aatacaaatc cagcataccg tcgcggaagt gcatgcccgc ataggaattc cggtagaaat
     4201 tgtcgagatg gggtttcggg tgctgaaaaa aatcctctat ccggtaatct tctcttcgga
     4261 ttattctgcc gcagaaaaac ttcaggtcta ccatttctcg attaacagta ttgatatcgc
     4321 tatggaagtg atgacccgcg cgtttacctt tagtgacagt agtgcctcaa aggaagatga
     4381 aaactatcgt atcttctcgt tactagaaaa cgccgaagaa gaaaaagaac ggcaaatagc
     4441 ctcaatactt tcatgggaaa tagatattat ctataaaatc ctgctggatt ctgatttagg
     4501 cagtagtttg cctttaagcc aggctgattt tggcctgtgg tttaaccata aaggtcgaca
     4561 ttattttagt ggcattgccg aagtaggcca tatctcccgt ctgattcagg atttcgacgg
     4621 tatattcaat caaaccatgc gtaacaccag gaatttgaat aacagaagtc tgcgggtgaa
     4681 atttttatta cagataagaa atactgtatc gcaaattatt accttgctgc gtgaattgtt
     4741 tgaagaagta tcgcgccacg aagtcggtat ggatgtactg acgaaattac ttaaccgccg
     4801 tttcctgccg actatcttca aacgcgaaat tgcccatgcc aaccggaccg gtacaccact
     4861 gtcagtgctg attattgacg ttgataaatt caaagagatc aacgatacgt ggggccataa
     4921 cactggcgat gaaattctgc gtaaagtctc tcaggccttt tatgacaacg tccgcagtag
     4981 tgattatgtt tttcgctacg ggggcgatga atttatcatt gttttgactg aagcttctga
     5041 aaacgaaacg ttacgtaccg cagaacgtat tcgcagtcgg gtggagaaaa ccaaactgaa
     5101 agccgcaaac ggcgaggata ttgccctctc actttccatc ggtgccgcca tgtttaatgg
     5161 tcatcctgac tatgagcgcc tcattcaaat agccgatgaa gctctgtata tcgccaaaag
     5221 acgaggtaga aaccgtgttg aactctggaa agccagtctt tagatgcgcc aggatgcaga
     5281 ggtaatcatg aagctaaccg atgcggataa tgccgccgat ggcatttttt tccccgccct
     5341 tgagcaaaat atgatgggtg cggtgttaat taacgaaaat gatgaagtga tgtttttcaa
     5401 ccccgccgca gagaagctct gggggtacaa acgtgaagaa gtcattggca ataacattga
     5461 tatgctgatt ccgcgggatt tgcgtcctgc gcatcctgaa tacattcgtc ataaccgtga
     5521 aggcggtaaa gcgcgtgtcg aggggatgag tcgggagctg cagctggaga aaaaagacgg
     5581 cagtaaaatc tggacccgtt ttgcgctatc gaaagtgagc gccgagggga aagtttatta
     5641 cctggcgctg gtacgggatg ccagcgtaga aatggcgcaa aaagaacaga ctcgacaatt
     5701 gattattgcc gttgaccatc tcgaccgacc ggtgattgtc ctcgatccgg aacgccatat
     5761 tgtgcagtgc aatcgcgcat ttaccgaaat gtttggttac tgcattagcg aagccagcgg
     5821 tatgcagccc gatacactcc tgaacattcc tgaattccct gccgataacc gcattcgttt
     5881 acaacagttg ctatggaaaa ccgcccgcga tcaggacgaa tttctgctgt tgacgcgcac
     5941 cggtgaaaaa atctggatta aagcctctat cagcccggtt tatgacgtgc tcgcgcatct
     6001 gcagaacctg gtaatgactt tctcggatat caccgaagaa cggcaaatcc gccagcttga
     6061 aggcaatatt ctcgccgcca tgtgcagcag cccgccattt catgaaatgg gggaaatcat
     6121 ttgtcgtaac atcgaatctg ttctcaacga atcgcatgtt tcgctgttcg cactgcgcaa
     6181 cgggatgccg atacactggg cgtcatcttc gcacggtgca gaagttcaaa atgcgcaaag
     6241 ctggtcagcg accattcgtc agcgtgatgg cgcgcctgcg gggatcctgc aaattaaaac
     6301 ctcgtcagga gcagaaacca gcgcctttat cgaacgcgtg gcagatatca gtcagcatat
     6361 ggccgcgctg gcgctggaac aggaaaaaag ccgccagcat attgaacaac tcatccaatt
     6421 tgatccgatg accggtctgc caaatcgcaa taacctgcac aattacctcg atgacctggt
     6481 cgacaaagcc gtctctccgg tggtgtatct catcggtgtt gaccatattc aggatgtgat
     6541 tgatagcctt ggctatgcgt gggccgatca ggcattgctg gaagtggtca atcgctttcg
     6601 tgaaaaactc aaaccggatc agtatctctg tcgtatcgaa ggtacgcagt ttgtcctcgt
     6661 gagcctggaa aacgacgtca gtaacattac ccagatcgcc gatgagctac ggaatgtggt
     6721 cagcaagccg ataatgattg acgataaacc attcccgctt accttgagta ttggcatcag
     6781 ctacgacgtg ggtaaaaacc gcgattacct actctccact gctcacaatg caatggatta
     6841 tattcgcaag aatggcggta acggctggca gttcttcagc ccggcgatga acgaaatggt
     6901 aaaagagcgt ttggttttag gcgcagcgct gaaagaagcg attagcaata accaactgaa
     6961 actggtttac cagccgcaaa tcttcgcaga aacgggtgaa ctgtacggca tcgaagccct
     7021 tgctcgctgg cacgatcccc agcatggtca tgtaccccct tcacggttta ttcctctcgc
     7081 agaagagatt ggtgaaatcg aaaatattgg gcgctgggtc atcgcggaag cttgccgtca
     7141 gttagcagaa tggcgtagcc agaatattca tatcccggcg ttatccgtga acttgtcggc
     7201 gctgcacttt cgcagtaatc agctgcctaa tcaggtgtct gatgcaatgc aagcctgggg
     7261 tattgacggc caccagctga cggtagaaat cacggaaagc atgatgatgg aacacgatac
     7321 cgaaatcttt aagcgcattc agatcctgcg tgatatgggc gtgggcttat cggtagatga
     7381 ttttggtacg ggcttttccg gattatcccg cttagtcagt cttccggtaa cggaaatcaa
     7441 aattgacaaa agttttgtcg atcgttgtct gaccgaaaaa cgcatccttg ccttacttga
     7501 agccattacc agcattgggc aaagcctcaa tttaaccgtc gtggcggaag gcgtcgaaac
     7561 caaagagcaa tttgagatgc tacgcaagat ccactgtcgc gttattcagg gatatttctt
     7621 ttcccgcccc ctacccgccg aagaaattcc aggctggatg agcagcgtgt taccgctgaa
     7681 aatctgacaa attcctctcg cccgcactcg cgggttttca tttaacgtga cactgtcact
     7741 taaatactgt gatttcagct acgattccgg gagattcctt ccttaacttc ccctcttgca
     7801 ccaaaactgc actacgctgc acaaatgttg atcaaaagtt aaaaaatata actttaagcc
     7861 agcattctgg cgcttatccc ggcatggcat gagatctgca taagcggaaa gcgcagcaat
     7921 ttttgtctta tacaggaacc gtttatgtcg gataccaccg aactggttga tttagccgta
     7981 atcttccctg atctggagat cgaattgaaa tacgcctgcg ctgataacat cacaggtaaa
     8041 gctatttatc agcaagcgcg ttgtctgcta cacaaggatg cgattaccgc gctggcgaaa
     8101 agtatcagta tcgcccagct gtcaggctta caactggtga tttacgatgc gtatcgccca
     8161 caacaagcac aggcgatgtt gtggcaagcc tgcccagacc cgcaatatgt tgttgatgtg
     8221 acggtcggtt ctaatcacag ccgtggcacg gcgatcgacc tgacgcttcg tgatgagcac
     8281 gggaacatcc tcgatatggg cgcagggttc gatgaaatgc acgagcgctc ccatgcctat
     8341 cacccttccg tcccgcccgc cgctcagcgc aatcggctgt tgctgaatgc gataatgact
     8401 ggtggcggtt ttgtcggtat ctccagcgaa tggtggcact tcgaattacc ccaggcagcg
     8461 agttaccctc tgctcgctga tcaattctct tgttttatat cccctggcac acagcacgtc
     8521 agttaatcag gaaactgtca tgaagagatc gatatcgttt cgtcccacat tgctcgcgct
     8581 cgtccttgcc acaaatttcc cggtttcgca cgccgccgta ccgaaagata tgctggtgat
     8641 tggtaaggcc gccgatccac aaaccctcga cccggcggta acaatagata ataacgactg
     8701 gacggtgacc tacccgtctt atcagcggct ggttcagtac aaaacggacg gtgataaagg
     8761 ctcaaccgac gttgaaggcg atctggcaag tagctggaaa gcgtctgacg atcaaaaaga
     8821 gtggacgttc accctgaaag ataatgctaa atttgccgat ggcacacctg tcactgccga
     8881 agcagtaaaa ctttcttttg agcggctact aaaaatcggc caggggccag cagaagcatt
     8941 tcccaaagat ttaaagattg atgctcccga cgaacatacg gtgaagttta cccttagcca
     9001 accattcgca ccgttcctct acacgctggc gaatgacggt gcatccatta tcaatccggc
     9061 ggtcttaaag gaacatgcag cggatgatgc tcgcggcttc ctcgcgcaaa ataccgccgg
     9121 ttccggacca tttatgctga aaagctggca aaaaggtcag caattagttc tggtgccaaa
     9181 tccgcattac cccggcaata aaccgaactt taagcgagta tcggtaaaaa ttatcggtga
     9241 aagtgcctcc cgtcgcctgc agctctcccg tggcgacatt gacattgccg atgcgctgcc
     9301 ggtggatcaa ctcaacgccc tgaagcagga aaataaagtc aatgtggcag agtatccgtc
     9361 actgcgcgtt acctatctgt atctgaataa cagcaaagcg cctcttaatc aggcggatct
     9421 acgtcgggcc atttcctggt ctaccgatta tcagggcatg gttaacggca ttctgagtgg
     9481 taacggaaaa cagatgcgcg gcccgattcc ggaaggcatg tggggctacg atgcgacggc
     9541 aatgcaatac aaccatgacg aaacgaaagc caaagccgaa tgggataaag tgacgagcaa
     9601 acccaccagc ctgacgtttc tctactccga taacgatccg aactgggagc ctattgcttt
     9661 ggcgacacaa tccagtctca acaagctggg tatcaatgtg aagctggaaa agctggcgaa
     9721 cgccaccatg cgcgacagag tgggtaaagg tgattacgac attgcgattg gcaactggag
     9781 tccggatttt gccgacccgt atatgtttat gaattactgg tttgagtcag acaaaaaagg
     9841 tctgccgggt aaccgctcgt tctatgaaaa cagtgaggtc gataagttac tgcgcaatgc
     9901 gcttgcgacc accgaccaga cgcagcgtac ccgggactac cagcaggcac agaaaatcgt
     9961 cattgatgac gctgcttatg tgtacctgtt ccagaaaaac taccaactgg cgatgaacaa
    10021 agaggtgaaa ggctttgtgt tcaatcccat gctggaacag gtcttcaata tcaataccat
    10081 gagtaaataa cgtgaccttc tggagtattt tacgccaacg ctgctggggg ctggtgctcg
    10141 tggtggcggg cgtctgcgtg attacgttta ttatctcgca tctgatccct ggcgatccgg
    10201 cgcggttact ggcgggtgac cgcgccagcg atgctatcgt ggaaaatatt cgccagcaac
    10261 tgggactgga ccagccactg tatgtacagt tttaccgcta cgtcagcgac ctgtttcatg
    10321 gtgacctggg aacatccatt cgtaccgggc gtccggtgct ggaagagttg cgtatatttt
    10381 tcccggcgac gctggagctg gcttttggtg ccctgctgct ggcactcctg attggcatcc
    10441 cgctgggcat actctctgca gtctggcgaa atcgctggct ggatcatctg gtgcgaataa
    10501 tggccattac cggaatctcc acacctgcgt tctggcttgg gctgggcgtc attgtgctgt
    10561 tttatggtca tctgcaaatt cttcccggcg gcggaaggct tgatgactgg ctggatccac
    10621 caacgcacgt taccggcttt tatctgctcg atgcgctgct tgaaggcaac ggtgaagtct
    10681 tcttcaatgc gttgcaacat ctcatcttac cggcattaac gctggcgttc gttcacctgg
    10741 gaattgtcgc tcgccagatc cgctcagcga tgctggaaca attgagtgaa gactacattc
    10801 gtaccgcccg ggccagcggc ttgcccggct ggtatatcgt tttatgttat gcgctaccca
    10861 atgcgttgat cccatcgatt accgtattgg gtttggcgct gggcgatttg ttgtatggcg
    10921 cagtgctcac cgaaaccgtt tttgcctggc ccggaatggg tgcatgggta gtaacatcaa
    10981 tacaggcgct cgacttcccg gcagtgatgg gctttgccgt cgtggtttca tttgcttatg
    11041 tgctggtcaa cctggtggtg gatttgctct atttgtggat tgatccgcgt atcggacgtg
    11101 gaggtggtga atgatgctaa gcgaggaaac gtccgccgta cgcccacaaa aacaaacgcg
    11161 atttaacggt gcaaaactgg tatggatgct gaaaggcagt ccgctcaccg tgaccggcgc
    11221 agtcatcatt gtattaatgc tattgatgat gattttttca ccgtggctgg cgacgcatga
    11281 tcccaacgcc attgatttaa ccgcccgcct tttgccgcct tctgcggcgc actggtttgg
    11341 caccgatgaa gtgggacgcg atctgttcag ccgcgtactg gtcggcagtc agcaatcaat
    11401 tctcgccgga ttagtcgtgg ttgccattgc gggtatgatt ggttcgctac tcggatgtct
    11461 atccggtgtg cttggcggac gcgcagacgc catcattatg cgcatcatgg acattatgct
    11521 gtcgattcct tcgttggtac tgacaatggc actggctgcc gctctcgggc cgagtttatt
    11581 taacgccatg ctggcgcttg ctattgtgcg aattcccttt tatgtgcgcc tggcgcgggg
    11641 gcaagcatta gttgtacgcc agtataccta tgttcaggcg gcgaaaacct ttggtgcgtc
    11701 gcgttggcat ctgatcagct ggcatatttt acgtaactcc ctgccgccgc tgatcgtgca
    11761 ggcatcgctg gatatcggta gcgcgatttt aatggccgcc acgttgggat ttattggcct
    11821 gggtgctcaa caaccgagtg ctgaatgggg cgcgatggta gcgaatggtc gcaactatgt
    11881 actcgatcag tggtggtatt gcgcatttcc gggggcagcg attttgctta ccgccgttgg
    11941 gtttaatctc tttggtgatg gtattcgcga tctgcttgac ccgaaagcag gaggaaagca
    12001 gtcatgaccc aacccgttct ggacattcaa cagctgcatt tgagtttccc cggttttaac
    12061 ggtgatgttc acgcgctcaa caatgtgtcc ttgcagatta accgcggcga aattgtcggt
    12121 ctggtgggag aatccggctc aggtaaatca gtcaccgcaa tgctgattat gcgtctgcta
    12181 cagacgggca gttattgcgt acatcggggg cagatttcac tgctgggaga cgatgttctt
    12241 aacgctcggg aaaaacagct tcgtcagtgg cgcggcgcac gagtggcgat gatctttcag
    12301 gaaccgatga ccgccctcaa tccgacactt cgaataggtc ttcagatgat ggacgtgatc
    12361 cgccatcatc aaccaataag tcgtcgggaa gccagagcta aagcgattgc cctgctggaa
    12421 gagatgcaaa tcccggatgc cgtggaagtt atgtcgcgct atccgtttga gctttcaggt
    12481 ggtatgcgcc agcgggtaat gattgcgctg gcattctcct gcgagccgca attgattatt
    12541 gccgacgaac cgactacggc gctggacgtc acggtgcagt tgcaggtact gcgtttgctt
    12601 aaacataaag cccgcgccag tggaactgcg gttctgttca tcagccatga tatggccgtg
    12661 gtgtcgcaac tgtgcgatag cgtttacgtg atgtatgccg gaagcgtaat tgaaagcggc
    12721 gtgacggcag atgttatcca tcatccccgg catccgtata ccattggttt gctccaatgc
    12781 gcaccagaac atggagtacc acgccagcta ttacccgcca ttccagggac ggtaccaaac
    12841 ctcacccatt tgcctgaagg ctgcgctttt cgcgatcgtt gctatgcggc aggtgcacag
    12901 tgtgaaaacg tcccggcgct gacagcgtgt ggtgacaaca accagcgctg cgcctgttgg
    12961 tatccgcagc aggaggtcat aagtgtctga cacgttatta acgttacgcg acgtccatat
    13021 caatttcccg gcccgtaaaa actggcttgg taaaactacg gaacatgttc atgccattaa
    13081 tggtattgat ttacagatcc gccgtggtga aaccttaggg atcgtcggcg agtcaggctg
    13141 cggcaaaagc accctcgcac agcttttaat gggcatgctg caaccgagcc acgggcagta
    13201 catccgttct ggctcacaac gcattatgca gatggtgttt caggacccgc tctcttcgct
    13261 caatccgcgc ttaccggtgt ggcgcatcat cacagaaccg ctctggatag ctaagcgtag
    13321 tagtgaacaa cagcggcgag cgttggcaga ggagctggct gtgcaggtgg gtattcgtcc
    13381 ggagtatctc gaccgcctgc ctcatgcgtt ctccggcggg cagcggcaac gcatcgccat
    13441 tgccagagca ctctcttcgc agcctgacgt gattgtgctt gatgagccaa cctcggcgct
    13501 ggatatctcc gtgcaggcgc agatcctcaa tttactggta acgctacagg aaaatcacgg
    13561 gctgacttat gtgctgattt cacacaatgt ctcggtgata cgtcatatga gcgatcgggt
    13621 ggcggtgatg tatctcgggc agattgtgga actaggggaa gcgcaacagg tgttgactgt
    13681 tccggcacat ccatataccc gattattgct ggattccctc cccgccattg ataaaccgct
    13741 ggaggaagaa tgggcattac gtaaaacgga tctgccggga aaccgcacgt tgccgcaagg
    13801 ctgttttttc tacgaacgtt gccctctggc aacccacgga tgtgaagtcc ggcaatcatt
    13861 aacaataagg gaggacggac gtgagatccg gtgctggcgg gcgctgtaga gatgctaaca
    13921 acgcatcagg cattcacgcg cctgacgcgt catccggcaa tgctttacga tttcaactgg
    13981 taatcaagcg taatttccgc tttcagtacc tgagaaaccg ggcatcctgc ttttgctttc
    14041 tggatgatgc cgtcaaaggt agaggcatca atacccggca ccgcaacttc actcttcagt
    14101 gcgattttcg taatcgcaaa accggcatcc actttatcca gcgacacatc ggcggtggta
    14161 tcaatcgatg ttggcgtaaa tcccgcttcc cccagcatta atgaaagcgc cattgagaaa
    14221 catgcggcat gcgctgcgcc aatcagttct tcagggttag ttcctttttc gccttcaaaa
    14281 cgcgtgttaa atccatacgg ctgttggttc agcacaccac tctcggtgga tactgttccc
    14341 ttcccgcgtt tgatatcgcc ttcccagtgt gcctgacctt tcttatggat tgtcattgtt
    14401 gctctcctgt gggcttaaaa aggaaattat agactacgtg agaaacagca cgaggataag
    14461 caggatattc cgaataaaaa tccggcagaa atattacttc tgcacttaaa ttaaatttcc
    14521 acaatagata accgggaata tataacccaa gagatattgc attaatatta atcaactcac
    14581 cacaataata atacctatca atatcatgag gttaaaatat aaaaatcatc aaccaggact
    14641 aatcttaaca acgaagcggc aaatatttgc catgctgaat gtgctttata aatctgcgat
    14701 ccgtagcaga caccataaat acacagacac ggagaatcac tatgtttact tattatcagg
    14761 cagaaaattc aacagcagaa cccgctctgg ttaatgccat tgagcaaggc cttcgggcag
    14821 agcacggtgt tgtcactgaa gatgacattc tcatggagct gaccaaatgg gtggaagctt
    14881 ctgataacga catcctcagt gatatctacc agcaaacgat caattatgtg gtcagtggcc
    14941 agcaccctac gctttaaggt gctatgcttg atcggcaacc taatttaggg gtttagcacg
    15001 tgtttcttcg ctacggcgat gttgtcctta aaactagcaa caggattgag gagttaaaat
    15061 gaaatcgaac cgtcaggcac gtcatattct tggactggac cataaaattt ctaaccagcg
    15121 caaaatagtt accgaaggtg acaaatccag cgtggtaaat aatccaaccg gcagaaaacg
    15181 ccccgctgaa aagtaattca taaccatcag tcctcaatga cgattaaaca ccattgcctg
    15241 cgcaatggtg tttttgtttt tatctgctct atacttgggg ccgacgccct ggcggtaaag
    15301 caaagacgat aaaagcgtgc cagggatgga tattcaaaaa agagtgagtg acatggaacc
    15361 aaaaacaaaa aaacagcgtt cgctttatat cccttacgct ggccctgtac tgctggaatt
    15421 tccgttgttg aataaaggca gtgccttcag catggaagaa cgccgtaact tcaacctgct
    15481 ggggttactg ccggaagtgg tcgaaaccat cgaagaacaa gcggaacgag catggatcca
    15541 gtatcaggga ttcaaaaccg aaatcgacaa acacatctac ctgcgtaaca tccaggacac
    15601 caacgaaacc ctcttctacc gtctggtaaa caatcatctt gatgagatga tgcctgttat
    15661 ttatacccca accgtcggcg cagcctgtga gcgtttttct gagatctacc gccgttcacg
    15721 cggcgtgttt atctcttacc agaaccggca caatatggac gatattctgc aaaacgtgcc
    15781 gaaccataat attaaagtga ttgtggtgac tgacggtgaa cgtattctgg ggcttggtga
    15841 ccagggcatc ggcgggatgg gcattccgat cggtaaactg tcgctctata ccgcctgtgg
    15901 cggcatcagc ccggcgtata cccttccggt ggtgctggat gtcggaacga acaaccaaca
    15961 gctgcttaac gatccgctgt atatgggctg gcgtaatcca cgtatcactg acgatgaata
    16021 ctatgaattc gttgatgaat ttatccaggc tgtgaaacaa cgctggccgg acgtgctgtt
    16081 gcagtttgaa gactttgctc aaaaaaatgc gatgccgtta cttaaccgct atcgcaatga
    16141 aatttgttct tttaacgatg acattcaggg cactgcggca gtaacagtcg gcacactgat
    16201 cgcagccagc cgcgcggcag gtggtcagtt aagcgagaaa aaaatcgtct tccttggcgc
    16261 aggttcagcg ggatgcggca ttgccgaaat gatcatcgcc cagacccagc gcgaaggatt
    16321 aagcgaggaa gcagcgcggc agaaagtctt tatggtcgat cgctttggcc tgctgacgga
    16381 caagatgccg aacctgctac ctttccagac caaactggtg cagaagcgcg aaaacctcag
    16441 tgactgggat accgacagcg atgtgttgtc actgctggat gtggtgcgca atgtaaaacc
    16501 agatattctg attggcgtct caggacagac cgggctgttt acggaagaga tcattcgtga
    16561 gatgcataaa cactgtccgc gtccgatcgt gatgccgctg tctaacccga cgtcacgcgt
    16621 ggaagccaca ccgcaggaca ttatcgcctg gaccgaaggt aacgcgctgg tcgccactgg
    16681 cagcccgttt aatccagtgg tatggaaaga taaaatctac cctatcgccc agtgtaacaa
    16741 cgcctttatt ttcccaggca tcgggctggg tgttattgct tccggcgcgt cacgtatcac
    16801 cgatgagatg ctgatgtcag caagtgaaac gcttgctcag tattcgccgc tggtgctgaa
    16861 cggcgaaggt ctggtactac cggaactgaa agatattcag aaagtctccc gcgcaattgc
    16921 gtttgcggtt ggcaaaatgg cgcagcagca aggcgtggcg gtgaaaacgt ctgccgaagc
    16981 tctgcaacaa gccattgacg ataatttctg gcaagccgaa taccgcgact accgccgtac
    17041 ctccatctaa gcctgcgccc ggtagtgaag gctaccgggc tatttccctc tccctttttc
    17101 agatctcatc catactgggt agtggcgaat aaatctcatt tgcctcacct gctatgcaga
    17161 acatcatccg aaaaggagga actatgaagg ctgcagttgt tacgaaggat catcatgttg
    17221 acgttacgga taaaacactg cgctcactga aacatggcga agccctgctg aaaatggagt
    17281 gttgtggtgt atgtcatacc gatcttcatg ttaagaatgg cgattttggt gacaaaactg
    17341 gggtaattct gggccatgaa ggcatcggtg tggtggcaga agtgggtcca ggtgtcacct
    17401 cattaaaacc aggcgatcgt gccagcgtgg cgtggttcta cgaaggatgc ggtcattgcg
    17461 aatactgtaa cagtggtaac gaaacgctct gccgttcagt taaaaatgcc ggatacagcg
    17521 ttgatggcgg gatggcggaa gagtgcatcg tggtcgccga ttacgcggta aaagtgccag
    17581 atggtctgga ctcggcggcg gccagcagca ttacctgtgc gggggtcacc acctacaaag
    17641 ccgttaaact gtcaaaaatt cgtcccggtc agtggattgc tatctacggt cttggcggtc
    17701 tgggtaacct cgccctgcaa tacgcgaaga atgtctttaa cgccaaagtg atcgccattg
    17761 atgtcaatga tgagcagtta aaactggcaa ccgaaatggg tgcagattta gcgattaact
    17821 cacgcaccga agacgccgcc aaaattgtgc aggagaaaac cggtggcgct cacgctgcgg
    17881 tggtaacagc agttgctaaa gctgcgttta actcggcagt tgatgctgtc cgtgcaggcg
    17941 gtcgtgttgt ggctgtcggt ctgccgccgg agtctatgag cctggatatc ccacgccttg
    18001 tgctggatgg cattgaagtg gtcggttcgc tggtcggcac gcgccaggat ctaaccgaag
    18061 ccttccagtt tgccgccgaa ggcaaagtgg tgccgaaagt cgccctgcgt ccgttagcgg
    18121 acatcaacac catctttacc gagatggaag aaggcaaaat ccgtggccgt atggtgattg
    18181 atttccgccg ttaagaggcc tttgctgcga gtgccagtcc tggtgtcgca gcatcgcact
    18241 ctctccgaca ttaaatggct aaatggatta ttgttccttc ctacgcaaca ataatgatag
    18301 tgttcctgat gaaggaacac tactgagagt aaagacactg atgaaaatgg ccaatcatcc
    18361 ccgcccgggg gacattattc aggaatcact ggacgaactt aatgtcagcc tgcgcgagtt
    18421 tgccagagca atggaaattg ctccctcaac ggcaagccga ttgctgaccg gaaaagcagc
    18481 tttgacacca gaaatggcga ttaaactttc cgtggtgatc ggcagttcgc cgcaaatgtg
    18541 gctgaatctg caaaacgcct ggagtctggc agaggcagaa aaaacggtgg atgtgtcgcg
    18601 actacgccgt ctggttacgc aataaaaaaa tggcgcttct taaagcgcca ttttcagctt
    18661 aaagttcaaa agagattata tcccttcttc actctctttt ttcgcttctg ccttctcgat
    18721 ttcacgatac cagcgcggat ggtgtttctt cgcccagcga cggctcacct tcccttcgat
    18781 catcccttta atcgaccctt tcacccagaa tgccatatac atatggatca ggatggcgtg
    18841 gatcaggatg atacccgcag ccgcgtggat cagcaggctg tagcgaacaa cctgcatcgg
    18901 gaagtactgc gcaaagtacg gacgccagat aatcaccccg gtcaccagca gcacgaaaat
    18961 catgctcatg atcgaccaga acatcatctt ttgcccggcg ttgtacttac cgacatccgc
    19021 cactttatgc tcattgcctt tcaatacttc gacaatgttc aacagccacg gaatatcttt
    19081 cttatccggg atgttgtgat gcacaaaacg cacaaacata aacatcagtg cgacgaaaat
    19141 cgcaatgccg aaaaacgggt gcaaaatgcg tcccatctgc ggcgtaccga aggtttgcgt
    19201 cagccattgc agcgtcggga agaagaacga aatcccggac agcgccacca ggaagaagca
    19261 aatcaccacg gtccagtgac aggcgcgatc aataaatttg gtgcgcacaa tcattttcga
    19321 cttactcatg atgatcctcc tcgtcatcgt ccacttcctt attcgggcca ataccgatgt
    19381 agtggaaaat caacccggca aaagtggcaa taaagccagc cgctgccagc ggtttcaacg
    19441 cgcctttcca caggcttacc gaggtgtcga tcttcggatc tttcggcaga ccgtgataca
    19501 gctccggctg atcggcgtga tgcagcacgt acataacgtg cgtaccaccg accccttccg
    19561 ggttgtagac gccagcatgt tcgtaaccac gcgctttcag tttcgccacg cgctgttccg
    19621 ccagctccag catctccttc ttggtgccga agtggatagc cccggtcgga caggttttca
    19681 cacaagccgg ttcctggccg acgctgacgc gatcgacgca gagcgtgcat ttatataccc
    19741 ggttatcctc tttgttgagg cgcggaatat taaacggaca cccggcaatg cagtaaccac
    19801 agccgatgca gttttccgac tggaaatcga caatcccgtt agcgtactga atgattgcac
    19861 cagcagacgg gcacgccttc aggcagccgg gatcttcaca gtgcatacag ccgtctttac
    19921 ggatcagcca ctccagcttg ccgttctgtt cggtttcgct aaagcgcatc accgtccagg
    19981 acttggcgct cagatcggcg gggttatcgt aaaccccgac gcagtgcccc acttcatcac
    20041 ggatgtcgtt ccactccgaa cacgccacct gacaggcttt acagccgata caggtggaaa
    20101 cgtcgataag ttttgcgact tctgctttgt aatcacgcac ctgagaaggc ggcgtgatgg
    20161 agttagttgc ggaccttttg ataatgtcct gcgtttccat agccatctgt tcgccccctt
    20221 acgccttctc gatgttgact aagaacgctt tatattccgg cgtttgcgag tttgcatcac
    20281 cgacattcgg cgtcagagtg ttagcgatat aacctttacg cgcgacaccc tcaaagcccc
    20341 agtggattgg aatacccacc gtttcaacct gctgaccatt tacattcagt ggtttcagac
    20401 gacgcgttac cacagccacc gcgcggataa agccacgctt gcttgagaca gtgacacgat
    20461 cgccattatt aatgcctttc gccgccgcca gcgtttcgct gatttccaca aactgttccg
    20521 gctgagcaat tgcgttgagc aatgcgtgct tggtccaggt gtggaagtgc tcggtcagac
    20581 gataggtcgt acccacatac gggaactgct cttttttacc catccgcagt gcgtcttgtt
    20641 catacagacg aacaaccggg ttagacacca cgttcggatg cagcgggtta gtgcccagcg
    20701 gcgtttcaat cggctcgtag tgttccggga acggaccttc cgccattttg ttgatggcaa
    20761 acaggcgtcc catcccttcc ggctgcatga taaacggccc ggttggcgta cccggtgcgg
    20821 cattgccgaa gtcaggaata tcgttacccg tccacttgct gccgttccac tggatcagca
    20881 tccgtttcgg atcccacggt ttaccgttga tatccgccga agcacggttg tacagcacgc
    20941 gacggttgag cggccacgcc caggcccatc ccagcgtatt ccccagaccg gacgggtcgg
    21001 agttatcgcg gttagccatc tggttgccct gctctgtcca gctaccggtg tagatccagc
    21061 aagaagatgc ggttgtaccg tcatcacgca gatgcgcaaa gctactcagc aactgacctt
    21121 tcttcgcaat cagcacgcca ttagcatcat agagatcttc cagcgcatag ccgttgttct
    21181 ctttagctac ttcgtcagat tgcggttcgt gcggctgctt gtagttccag ctcatcttca
    21241 tcagcggttc tacgccttta ccaccttcgg cctggtacag ctcgcgcagg tgatggtaga
    21301 tacccgccag aatttcaccg tcgttacgcg cttcgcccgg cgcatcctga cctttccagt
    21361 gccactgcag ccagcgaccg gagttagcaa tagaaccatc ttcttcagca aagcaggtcg
    21421 aaggcagacg gaatacttca gtctgaatag acgccggatc gacatcgttc gactcgccgt
    21481 ggttctgcca gaaggttgag gtttcagtca ccagcggatc gataaccacc atgtacttca
    21541 gcttgcttag gcagctcacc actttgtttt tgtccgggaa ggacgcaacc gggttaaagc
    21601 cctggcagaa ataaccggtg actttgcctt catccatcat gttgaaatac ttgatgacgt
    21661 cgtaggtctg gtcccacttc ggcagccagt catagcccca gttgttctct ttctgcgcgg
    21721 catcgccata gaaagatttc atcaggctaa cgaagaactt cggatagttg ctccagtagt
    21781 tcacctgacc agccagcgtc gctttcggcg tgttcgcttc cagatacgac tgcaaatcaa
    21841 cctgtttttc tgacggcagc gtcagataac ctggcaggct ggtagagagc agacctaagt
    21901 cagtcagacc ctgaatgttg gagtgaccac gcaatgcatt cacgccgcca ccggccatac
    21961 ccatgttacc gagcagcaac tggatcatcg ccatagtacg gatgttctgc gcacccacgg
    22021 tgtgctgcgt ccagcccagc gcgtacagga aggtggttgt gcgatccggt gcgctggtgg
    22081 aggccagcac ttcacacact ttcaggaagt cggcttttgg cgtaccacag atgttttcaa
    22141 caacgtccgg cgtgtagcgg gaaacgtgcg ctttcagcag gttccacaca cagcgcggat
    22201 gagtcagtgt ttcatcgcgt ttcgcatagc cgttttcatc gaactgatag ttccaggacg
    22261 atttatcgta ttgccgtttt tcagcgtcgt agccgctgaa cagaccgtct tcgaaagcaa
    22321 aatcatcacg caccagcagg ctggcgttgg tgtaatgctt aacgtattcg gcgttgattt
    22381 tgtcgttttc gatcaggtag cgcaaaacgc cagacaggaa cgtaatgtcc gtaccggaac
    22441 gaataggtgc gtaaatatcc gccacagaag cggtacgcgt aaaacgagga tcgacaacga
    22501 tcaaggttgc atcgttgttg tttttcgctt ccatcgccca gcggaaaccg acgggatgcg
    22561 cttcagcagc gttaccgccc attaccatta cgacgttagc gtttttgata tccacccagt
    22621 ggttggtcat cgcaccgcga ccaaatgttg gagcaagact tgctaccgtt ggtccgtgtc
    22681 agacgcgcgc ctggttgtct accgccagca tcccgaggga gcgggcaaat ttctgtgtca
    22741 gcatcccggt ttcgttgctg gcaccggagg cacacagcat accggtagaa agccaacggt
    22801 ttaccgttac gccctgctcg ttcttttcaa taaagttagc gtcacggtca gctttcatca
    22861 gctttgcaat acgggagaat gcttcttccc agctaatgcg ctgccatttg tcagaacctg
    22921 gcgcacgata ttccgggtag cgcagacggt tttcgctgtt gacgtaatcc agcaaaccgg
    22981 ccccttttgg gcacagcgca ccacggctta ccggatgatc cgggtcacct tcaatgtgat
    23041 aaatcgcttc tctggcgttt ttcgcgccat cacccaggct atacatcaat agcccgcaac
    23101 ctacggaaca gtatgtgcag gtgttacgga tctctttagc gcgtaataat ttgtagtttc
    23161 gcgcctgagc cagtgcttgc ttcggggcaa agcccaatgc cgctaccgtt gttccagcca
    23221 taccgcccgc gcagatttta aaaaattgtc tgcgactgac gtccattgct ttcctctttt
    23281 ttcaggggta ttactgcgcg aggaaactaa cagtaaaatt ctgaatcttt ttgcgcgaaa
    23341 tcaaaaagaa cacgtgtttg ccacaaaata atccgcctgc gcaaattgac ctacctcaat
    23401 agcggtagaa aaacgcacca ctgcctgaca ggccagttaa aaaaatgcta taaaattcag
    23461 cttaattttt aacggcaaga gagacaaaac agcgagcatg acacgacaaa aagcaacgct
    23521 catagggcta atagcgatcg tcctgtggag cacgatggta ggattgattc gcggtgtcag
    23581 cgaggggctc ggcccggtcg gcggcgcagc tgctatctat tcattaagcg ggctgctgtt
    23641 aatcttcacg gttggatttc cgcgtattcg gcagattccg aaaggctatt tactcgccgg
    23701 gagtctgtta ttcgtcagct atgaaatctg tctggcgctt tccttagggt atgcggcgac
    23761 ccgtcatcag gcgattgaag tgggtatggt gaactatctg tggcccagcc tgacaattct
    23821 ctttgccatt ctgtttaatg gtcagaaaac caactggctg attgtaccag gattattatt
    23881 agccctggtc ggcgtctgct gggtgttagg cggtgagaat gggttacatt atgatgaaat
    23941 catcaataat atcaccacca gcccattgag ttatttcctg gcgtttattg gtgcgtttat
    24001 ctgggcagcc tattgcacag taacgaataa atacgcacgc ggatttaacg gaattaccgt
    24061 ttttgtcctg ctaacgggag caagtctgtg ggtttactat tttctcacgc cacaaccaga
    24121 aatggtattt agcacgcccg tcatgattaa actcatctct gcggcattta ccttaggatt
    24181 tgcttatgct gcatggaatg tcggtatact gcatggcaat gtcaccataa tggcggtagg
    24241 ctcgtatttt acgcccgtac tttcctcagc gcttgcagcc gtgctgctca gcgccccgct
    24301 gtcgttctca ttctggcaag gcgcgctaat ggtctgcggc ggttccctgc tctgctggct
    24361 ggcgacacgt cgtggttaaa ataa
//
LOCUS       DAZGLJ010000052        22906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000052 DAZGLJ010000000
VERSION     DAZGLJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.052"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..164
                     /gene="xisR"
                     /locus_tag="ACHK49_001867"
     CDS             <1..164
                     /gene="xisR"
                     /locus_tag="ACHK49_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO9262526.1"
                     /translation="KKAREKSFMEGREYKHVAHDGMPWDNSPCFYNLEEIDRWIERQA
                     SARPRRHLT"
     gene            199..1479
                     /locus_tag="ACHK49_001868"
     CDS             199..1479
                     /locus_tag="ACHK49_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9262527.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTSKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSKFAPTMPHNEAMKN"
     gene            complement(1667..2686)
                     /gene="rspB"
                     /locus_tag="ACHK49_001869"
     CDS             complement(1667..2686)
                     /gene="rspB"
                     /locus_tag="ACHK49_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO9262528.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(2698..3912)
                     /gene="rspA"
                     /locus_tag="ACHK49_001870"
     CDS             complement(2698..3912)
                     /gene="rspA"
                     /locus_tag="ACHK49_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO9262529.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(4118..4444)
                     /gene="ynfA"
                     /locus_tag="ACHK49_001871"
     CDS             complement(4118..4444)
                     /gene="ynfA"
                     /locus_tag="ACHK49_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO9262530.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            4579..4920
                     /gene="ynfB"
                     /locus_tag="ACHK49_001872"
     CDS             4579..4920
                     /gene="ynfB"
                     /locus_tag="ACHK49_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO9262531.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            4955..5515
                     /gene="speG"
                     /locus_tag="ACHK49_001873"
     CDS             4955..5515
                     /gene="speG"
                     /locus_tag="ACHK49_001873"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO9262532.1"
                     /translation="MPSAHSVKLRPLEREDLRYEHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(5518..6228)
                     /gene="ynfC"
                     /locus_tag="ACHK49_001874"
     CDS             complement(5518..6228)
                     /gene="ynfC"
                     /locus_tag="ACHK49_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO9262533.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            6336..6641
                     /gene="ynfD"
                     /locus_tag="ACHK49_001875"
     CDS             6336..6641
                     /gene="ynfD"
                     /locus_tag="ACHK49_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO9262534.1"
                     /translation="MKLSTCCAALLLALASPVVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            6840..9265
                     /gene="ynfE"
                     /locus_tag="ACHK49_001876"
                     /pseudo
     CDS             6840..9265
                     /gene="ynfE"
                     /locus_tag="ACHK49_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
     gene            9326..11749
                     /gene="ynfF"
                     /locus_tag="ACHK49_001877"
                     /pseudo
     CDS             9326..11749
                     /gene="ynfF"
                     /locus_tag="ACHK49_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
     gene            11760..12377
                     /gene="ynfG"
                     /locus_tag="ACHK49_001878"
     CDS             11760..12377
                     /gene="ynfG"
                     /locus_tag="ACHK49_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO9262535.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            12379..13233
                     /gene="ynfH"
                     /locus_tag="ACHK49_001879"
     CDS             12379..13233
                     /gene="ynfH"
                     /locus_tag="ACHK49_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO9262536.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            13276..13890
                     /gene="dmsD"
                     /locus_tag="ACHK49_001880"
     CDS             13276..13890
                     /gene="dmsD"
                     /locus_tag="ACHK49_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO9262537.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            14049..15341
                     /gene="clcB"
                     /locus_tag="ACHK49_001881"
     CDS             14049..15341
                     /gene="clcB"
                     /locus_tag="ACHK49_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO9262538.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(15294..15989)
                     /gene="bioD"
                     /locus_tag="ACHK49_001882"
     CDS             complement(15294..15989)
                     /gene="bioD"
                     /locus_tag="ACHK49_001882"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO9262539.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(16114..17334)
                     /gene="mlc"
                     /locus_tag="ACHK49_001883"
                     /gene_synonym="dgsA"
     CDS             complement(16114..17334)
                     /gene="mlc"
                     /locus_tag="ACHK49_001883"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO9262540.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(17469..18362)
                     /gene="ynfL"
                     /locus_tag="ACHK49_001884"
     CDS             complement(17469..18362)
                     /gene="ynfL"
                     /locus_tag="ACHK49_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9262541.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRYTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            18469..19722
                     /gene="ynfM"
                     /locus_tag="ACHK49_001885"
     CDS             18469..19722
                     /gene="ynfM"
                     /locus_tag="ACHK49_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9262542.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(19771..19890)
                     /locus_tag="ACHK49_001886"
     CDS             complement(19771..19890)
                     /locus_tag="ACHK49_001886"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO9262543.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            20146..20454
                     /gene="asr"
                     /locus_tag="ACHK49_001887"
     CDS             20146..20454
                     /gene="asr"
                     /locus_tag="ACHK49_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO9262544.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            20547..20630
                     /locus_tag="ACHK49_001888"
     CDS             20547..20630
                     /locus_tag="ACHK49_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9262545.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            20730..21551
                     /gene="ydgD"
                     /locus_tag="ACHK49_001889"
     CDS             20730..21551
                     /gene="ydgD"
                     /locus_tag="ACHK49_001889"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO9262546.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(21590..21919)
                     /gene="mdtI"
                     /locus_tag="ACHK49_001890"
     CDS             complement(21590..21919)
                     /gene="mdtI"
                     /locus_tag="ACHK49_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO9262547.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(21906..22271)
                     /gene="mdtJ"
                     /locus_tag="ACHK49_001891"
     CDS             complement(21906..22271)
                     /gene="mdtJ"
                     /locus_tag="ACHK49_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO9262548.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            22683..22826
                     /locus_tag="ACHK49_001892"
                     /pseudo
     CDS             22683..22826
                     /locus_tag="ACHK49_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pheromone autoinducer 2 transporter"
BASE COUNT         5936 a         5749 c         5500 g         5721 t
ORIGIN      
        1 taaaaaaggc ccgggaaaaa tcgtttatgg aaggaaggga atataagcat gtcgctcatg
       61 acggtatgcc atgggataac agtccatgct tttataacct ggaagaaatt gatcgctgga
      121 ttgagcgcca ggcatcagcg agaccaagac gtcatcttac ttgactaaaa gccacactaa
      181 ctaatgagag aagttgaaat gaaatatccg acaggcgtgg aaaaccatgg agggaaatta
      241 cgtatctggt ttgtttataa aggcgtaaga gtcagggaaa atctgggggt tcctgacaca
      301 tcaaaaaaca ggcgcgttgc aggtgaacta cgctcctctg tttgttacgc aataaaaact
      361 ggtgttttcg actatgcaaa acagtttccc tcctcacgca atctggaaaa atttggtgag
      421 gcccgacaag atttaaccat aaaagaactg gctgaaaaat ttttggcact gaaagaaact
      481 gaagtcgcca aaacatcact caacacatac cgtgccgtca tcaaaaatat cctgagcata
      541 atcggtgaaa aaaatcttgc ctcatcgatt aataaagaaa aattactgga ggttcgtaaa
      601 gagctactga ctggatacca gatccccaaa agtaactata ttgttacaca accagggaga
      661 tcggctgtaa ctgtaaataa ttacatgaca aatcttaacg ccgtgttcca gtttggtgtt
      721 gataacggtt acctggcaga taatccgttt aaggggatct cgccattaaa ggaatcaaga
      781 accattccgg atcctctttc gcgggaggaa tttatccgtc ttatcgatgc gtgcagaaat
      841 cagcaagcaa aaaatttatg gtgtgtttct gtttatactg gcgttcgccc tggtgagctg
      901 tgtgcacttg gatgggagga catagatctg aaaaatggaa caatgatgat caggagaaat
      961 ttagcaaaag accgtttcac ggtaccaaaa acacaggcgg gaaccaatcg ggtcattcat
     1021 cttattaagc cagcaatcga cgctctccgg agtcagatga cattaacgag actgagcaaa
     1081 gagcatatca ttgatgttca cctcagagag tatggcagaa cagaaaaaca aaaatgcacc
     1141 tttgtttttc aacctgaagt gtcagcgaga gtaaaaaatt atggtgacca ttttaccgtt
     1201 gactcaataa ggcagatgtg ggacgcagcg ataaaacgtg ccggactccg ccatcgaaaa
     1261 tcatatcagt cgagacatac ttatgcctgc tggtcgctga cagctggtgc taacccggca
     1321 tttatagcaa accagatggg ccatgcagat gcgcaaatgg tatttcaggt atacggaaaa
     1381 tggatgtctg aaaacaataa tgcacaggta gctttgttaa atacacagtt aagcaagttt
     1441 gccccaacca tgccccataa cgaagcaatg aaaaattaat ttaatattta tcaaatagtt
     1501 aacacgcatg actcttgaaa tccataaatt caagcgcagt gcccagccat cccgatactg
     1561 ctgctttcac caaatcctta gtgcttcttt catgtttttc tattgtcata atggttatct
     1621 ctaaaaaaga ggtaagatgc gtactactta ctcgccgtta ttggtattat tcagaaaaag
     1681 tgagcaagac tttgcagcaa tgtttttgat cctgttcaaa taaactaatg gcatcagcaa
     1741 catgctggaa atcaaacgta tgggtaatta atttttctgg tttaattaac cctttactta
     1801 accagtcgat aacaaccgga aatttatttg catttaagcg tgaagagaaa atagagagtt
     1861 cttttccggt aattccttgc tgaatcactt cagacggttc actggagaag cccatcaata
     1921 caatacgtgc cgctggagaa gccagcgtta cggcctcttt caggatagaa ggatgacaag
     1981 ccgcatcgat aattaatgtc ggcttgatgc ctttttcagc gaaaatctcg ccaagcggtg
     2041 tctggctgtt attaatcgcc cagtctgccc cgctctcttt cgctttttcc agtcgttcat
     2101 caatgcgatc ggcaacaatc acatttttaa cgttatagac gccttttaat acctgaacga
     2161 tcgtcaggcc gattggaccg gcaccataaa ccagaacggt atcattttca gtcggttgac
     2221 catgaccggt aacgttagcc gcaatggtaa aaggttcgat catcaccgca tattgatcgg
     2281 ccactgcttc aggaattttc cacgcatttt ttgccggaac cacggcatat tcactgaaac
     2341 caccgtcagc gtgcacacct aatacagcaa gtgtcgtaca aacgttcggc ttacctatag
     2401 agcacggata gcaatgccca cagctgacca ccggatcgac agcaacgcgc tcaccgactc
     2461 tggcgctttc cacaccttca cccaccgcat caatgacgcc aaagaattca tgaccaatga
     2521 cgcgcggata tttcgcaaaa ggattatgcc cacggtaaat atggctatct gaaccacaaa
     2581 ttccggcaag tttcactttt actcgtactt cacccgctga cggggtgggt atttcacgtt
     2641 cgataatcga cagttgattc ggtttttcaa ttaaaatgct tttcattacc ttactcctta
     2701 ccagttccac agcgtgccat cttccagacg tgcgactggc agataagcag gttcataggg
     2761 atatttcgcc gccagctttt catcgaattc gatgccaaga cccggtttgt ctcccggatg
     2821 catatagccg ttatcgaaag tccagttgtg cgggaagact tcgagcattt gttcggaata
     2881 acccatgtac tcctggacac cgaaattggg gacccacaga tcaaagtgca gcgccgcagc
     2941 catgcagact ggtgacaaat cggaaggacc gtgtgagcca gtacgtacct gatacagcga
     3001 agcaaaatcg gcaatccggc gcataccggt aattccgcct gcatgggtca gcgtggtgcg
     3061 gatataatcg atgagttgct cttcaatcag ttgtttgcag tcccagatgc tgttgaagac
     3121 ttcacccact gcgatgggtg tgacggtatg ttggcgaatg agacggaagc attcctggtt
     3181 ttccgcaggc gtcgggtctt ccatccagaa catgcgataa tcttcaatgc ttttaccaaa
     3241 gcgcgccgct tcaataggcg ttaagcgatg gtgcatgtca tgcagcaaat gttcattaaa
     3301 accaaacttg ttacgtaccg cgtcaaacaa tttcggcatg aaatcgaggt atttctccgt
     3361 cgaccacagc tgctcttccg gccactgtcc tttggttgcg ggttcataag ccagaccttt
     3421 acctttcgac atgccgtagg tggttttcat accagggatt ccgcactgca cgcggatggc
     3481 tttgaatccc agctcctgat gacgggcata atcatccaga gcttcatcaa tactgtgacc
     3541 ggtggtatgg caataaacca tcaccccttc acgagacgcg ccgccgagta actggtaaag
     3601 cggcatgttg gcagctttgg ctttaatatc ccacagcgcc atatcaaccg ctgaaatggc
     3661 cgacatcgta accggaccgc gacgccagta agcaccttta tagaaaaact gccagatatc
     3721 ttcgatacgg tgcgcatcgc gaccaataag ctgcggacaa aggtgatcct gcaaataaga
     3781 ggccacggaa agctcacgtc cattgagggt ggcatcccca aggcccgtaa taccgtcctc
     3841 agtggtgatt tttaatgtga cgaaattacg ccccggacag gtaacaaaaa cttcagcctt
     3901 tacgatcttc atgttcgatt ccttgcatcg cttgtcgtga tgcatgaaat ctacgcaact
     3961 gagctactac catacaagca taaagatcga aaaaagccgg agtgatcaca aaaaaaggcg
     4021 tatatttgcg ctgtgaatgg ttgacaaaag atgaaataga ataccttttg tcagctgaca
     4081 cttcctctta tcttattgat aaaatggatt tatgttccta cgtgcgcccc cagcccgcaa
     4141 caatgatcaa catgccgcaa agcgcaatca acgcacccgt ccagtcataa agagtcagtt
     4201 tcacgccatc cacaacgcgc agccacatca acgccgtgca gacataaacg ccaccataag
     4261 ccgcgtaaac acgcccactc gccgctggat gcaacgttaa caaccagaca aacagcgcca
     4321 gtgaaatccc cgccggaagc aacagccaga tactggcgtt tcgttttaac cacaaccagg
     4381 gcagaaagca tccaataatt tcacacagcg cagtagcaaa aaatagtaac gttgttttaa
     4441 tcatctttgt ctcttattga catcatgtat agttataggg cgacataata tcatcaatat
     4501 aaacaccctc ctggtacgtt ttgcgtccgc agtggatggt gtagaatcac ctttaatcat
     4561 tcatacaggg aatgaattat gaaaatcact ctcagcaaac gaatcggcct gctcgctatt
     4621 ctgctgcctt gcgcactggc attgagcaca actgttcatg ccgaaactaa caaactggtg
     4681 attgagtctg gcgacagtgc acaaagccgc cagcacgccg ctatggaaaa agagcaatgg
     4741 aatgacacgc gcaatctgcg ccagaaagtg aataaacgca ctgaaaaaga gtgggataaa
     4801 gccgacgccg cttttgataa ccgcgataaa tgtgagcaaa gcgccaacat caatgcctac
     4861 tgggagccca atactttgcg ctgcctggac cgtcgaactg gccgcgttat taccccctaa
     4921 cctgttattg atttaaggaa tgtaaggaca cgttatgcca agcgcccaca gtgttaagct
     4981 acgcccgctg gagcgtgaag atttacgcta tgaacatcaa ctcgacaaca acgccagtgt
     5041 gatgcgttac tggtttgagg aaccctacga agcctttgtt gaactctctg atctgtatga
     5101 taagcatatt cacgatcaga gcgaacggcg ctttgtggtg gaatgtgacg gcgaaaaagc
     5161 cggtctggtg gagctggtgg aaattaacca tgttcatcgc cgcgcagaat ttcagataat
     5221 tatctccccg gagtatcagg ggaaaggtct ggcaacccgt gccgccaaat tagcaatgga
     5281 ctatggcttt accgttctca atctctataa gctgtatctg atcgttgata aagagaatga
     5341 aaaagcgatt cacatttacc gcaagcttgg cttttcggtt gaaggtgaat tgatgcacga
     5401 gttctttatt aatggtcaat atcgtaatgc cattcgcatg tgtatattcc agcatcagta
     5461 tctggcagag cacaaaacac cgggtcagac tctcctgaag ccgaccgcac aatagcatta
     5521 ataataatcg atcgtatttt tgatggtgta aacccgttcg acggcgggtt ttactccttc
     5581 atcaacaatg attagctgac agtccaccgg attagcgtga ctgtcatatt cacagctctg
     5641 ttttacatta ccaacccgtt gattattcag taaagtaacc gctgtgtaat ctaatttttt
     5701 gatcggatcc gttgatggcg tggcgctgac agataatgtt ttgtcgttac ttttcgtggt
     5761 tttacccagc ggataaccct gatcatcata gcgatattcc atctgcattt gtttgctgct
     5821 ggctttaatc acgaagccat tatcatcggt ttcccaactc accccggcag aaggtaattc
     5881 tgctagctgg cattttccct gtaaacgtac tctcttctcc agcgtctcgg catcacggta
     5941 ataattggcg tccagtacca gagcgaccac ggtattattt tccagatcca gtaattcgag
     6001 tgaatcaaaa cagccttctt ccgacaaagt cccagaaaca cgtttcgtca cttcaccttg
     6061 ctcatccatt aatgtctgag tgaaatcttt taccggacca cgcagcggat caaaatcgaa
     6121 ttcattagag aaactggcca tctcaggggt aaatgaaagt gttacctctg tgcggtcaca
     6181 tcctgtgagg aatatcgcga gtaagcatgg tagtaatttg tatttcacaa cagtcaccag
     6241 agagtagaga tgattctcaa tcatagtagc aaatacagta ctttacacgt taaatgctat
     6301 gcttaaagaa gttatcttcg cgtaaggagc ttatgatgaa actctcaacc tgctgtgccg
     6361 cgcttctgct cgccctcgcc tcacccgtgg tactcgccgc gccgggatcc tgtgagcgca
     6421 tacagagcga tatatcacag cgcattatca ataatggcgt acccgaaagc agcttcacgt
     6481 taagtattgt acccaatgac caggttgatc agcctgattc ccaggtcgtc ggccattgcg
     6541 ctaatgatac gcataaaatt ctctataccc gcaccaccag cggtaacgtc tctgctcccg
     6601 cgcagtctac ccaggatggc gcgcctgccg aaccgcagta atacattatc gactgaacgc
     6661 cggatacgac acatccggcg atttgaacat acaacataat cccaccttat tactcatacc
     6721 cttctattga tatggattaa taattcttaa cccaaaatgg gtagactccc tctattgtta
     6781 gcgcgctaaa tattcaatat ataaacattt atataacgat aaagaacagg gagtgagtta
     6841 tgtccaaaaa tgaccgaatg gtgggcatca gccgcagaac actcgttaaa tcaaccgcga
     6901 taggttctct ggcgctggct gcaggcggtt tttctttacc gttcaccctg cgcagtgcag
     6961 cggcaacggt acaacaggcc agcgaaaaag tgatctgggg tgcctgttcc gtcaactgtg
     7021 gtagccgctg tgcacttcgt ctacatgtta aagataatga agtgacctgg gtggaaactg
     7081 acaataccgg cagcgatgag tacggcaacc atcaggtacg cgcctgtttg cgcggtcgct
     7141 ccatccgccg gcgtattaat catcccgatc gcttgaatta cccaatgaaa cgcgtgggta
     7201 cacgcggtga aggcaaattc gaacggatta gctgggatga agccctggat actatcgcca
     7261 gtagcctgaa aaaaaccgtc gaacaatatg gcaatgaggc tgtatatatt cagtactctt
     7321 cggggattgt tggcggcaat atgacccgct cttcgccatc agcctcggcg gtcaaacgcc
     7381 tgatgaactg ctacggcggt tcactcaatc agtatggctc ctacagcacc gcgcaaattt
     7441 cctgtgccat gccctacacc tacggcagta atgatggcaa cagcaccacg gatattgaaa
     7501 acagcaagct ggtcgtgatg tttggtaaca acccggcaga aacccgcatg agcggcggtg
     7561 gcatcactta tcttcttgaa aaagcgcgcg agaaatcgaa cgccaaaatg attgttatcg
     7621 atccgcgata taccgatacg gctgctggtc gtgaagatga atggctccct attcgcccgg
     7681 gcaccgatgc cgcgctggtt gcgggtattg cctgggtatt gattaacgaa aatctcgttg
     7741 atcaaccttt tctcgataaa tactgcgtcg gctatgacga aaaaacctta cccgcagatg
     7801 cacccaaaaa tggtcactat aaagcctata ttcttggtga aggtgatgat aacacagcga
     7861 aaacgccgca gtgggcttcg caaattaccg gtatcccggt agaccgtatc atcaaactgg
     7921 cgcgtgaaat tggcacagca aaacccgctt atatctgcca gggctggggg ccgcaacgcc
     7981 aggcaaacgg cgaactgact gcacgcgcta ttgctatgtt acctattttg acgggcaatg
     8041 tcggcatcag cggcggaaat agtggcgcgc gtgaatcgac ctataccatt accatagaac
     8101 gcctgccggt gctggataat ccggtcaaaa cgtcaatctc ctgcttcagc tggacagatg
     8161 ctatcgatca tggtccgcaa atgacggcaa tccgcgacgg cgtccgcggc aaagataaac
     8221 tggatgtgcc cattaagttc atctggaact acgcgggaaa taccctcgtt aatcagcatt
     8281 ctgacatcaa caaaacgcat gaaattctgc aggacgaatc gaaatgcgaa atgattgtga
     8341 tcatcgaaaa ctttatgacc tcatcggcaa aatatgccga cattctgctg ccagacctga
     8401 tgaccgttga gcaggaagat attattccta acgactacgc cggtaacatg ggatatctca
     8461 ttttcctcca gcctgtcacc agcgaaaaat tcgaacgcaa accgatttac tggatcctga
     8521 gtgaagtcgc gaaacgtctt ggacctgacg tctatcaaaa gttcacagaa ggtcgcacgc
     8581 aggaacaatg gttacaacat ctgtacgcca aaatgcttgc caaagatccg gcgttaccgt
     8641 cttacgacga actgaaaaaa tgggtatcta taagcgtaaa gatcccaatg gccattttgt
     8701 cgcctacaaa gcatttcgtg acgaccccga ggcaaatcca cttaaaacgc cttccggtaa
     8761 gattgaaatt tattccagca ggctggcgga aattgcccgt acctgggaac tggaaaaaga
     8821 tgaagtgata agcccattgc cagtttatgc ctcaaccttt gaaggctgga actcccctga
     8881 acgtagaacc ttcccactgc aactgtttgg tttccattac aaatcccgta ctcactcgac
     8941 ctacggcaat attgatctcc tgaaggctgc ctgccgtcag gaggtgtgga tcaaccctat
     9001 agatgcgcag aaacgtggga ttgccaacgg cgatatggtg cgggtgttta accatcgtgg
     9061 cgaagttcgg ctaccagcca aagtaacacc acgtattctc cctggagtta gcgctatggg
     9121 ccagggagcc tggcacgagg ccaatatgtc tggcgataaa atcgaccatg gcggctgtgt
     9181 gaatacgcta accactctgc gcccttcacc actggcgaag ggaaacccgc agcacactaa
     9241 tctggtcgag atcgaaaaaa tataacccac gacaaccata aattctggca tgacattttg
     9301 tttgaaaagc aataagtgag taatgatgaa aatccatacc acagaggcgc tcatgaaggc
     9361 tgaaatcagc cgtagaagtc tgatgaaaac ctccgcactt ggcagtcttg cgctggcaag
     9421 cagtgctttc actctgccat tttcccaaat ggtccgggcg gcagaggctc cggtagaaga
     9481 gaaagcggtc tggagttcct gcaccgttaa ctgcgggagc cgctgtctgt tacgtttgca
     9541 tgtgaaagat gacaccgtgt actgggtgga gtctgatacg acaggtgacg acgtctacgg
     9601 taaccatcag gttcgagcgt gtttacgcgg gcgctctatc cgccgacgga tgaatcatcc
     9661 tgataggttg aaatatccca tgaagcgcgt cggcaagcgc ggtgaaggtt aatttgaacg
     9721 gataagttgg gacgaagccc tggataccat cagtgataat cttcggcgga tcctgaaaga
     9781 ttacggcaat gaggctgtac atgtcctgta cggaacaggc gtagatggcg gaaacatcac
     9841 caactcaaac gtcccgtacc gtctgatgaa ctcttgcggt ggttttctca gtcgctatgg
     9901 cagctacagt accgcacaga tcagtgccgc aatgagttat atgttcggtg ccaatgatgg
     9961 caacagcccg gatgatatcg ccaatacgaa actggtcgtt atgttcggaa ataacccggc
    10021 agaaacgcgg atgagcggcg gtggtgtcac ttactacgtc gagcaagccc gcgaacgttc
    10081 aaacgcacgc atgatcgtca tcgatccacg ttataacgac actgctgccg ggcgtgaaga
    10141 tgaatggctg cccattcgcc ctggcaccga tggcgcactg gcctgtgcga ttgcctgggt
    10201 actgattact gaaaacatgg tcgatcagcc atttctcgac aaatattgtg ttggttacga
    10261 tgaaaaaacg ctgcccgcca acgcaccacg taacgcgcat tataaagcct atattctggg
    10321 cgaagggcct gacggcatag ctaaaacgcc ggaatgggca gcaaaaatca ccagcatccc
    10381 ggcagaaaaa attatccagt tggcacgaga gatcggttca gcaaaacctg cttatatttg
    10441 tcagggttgg gggccacaac gacattccaa cggcgagcaa acgtcccgcg ctattgccat
    10501 gctttccgtt ctcaccggta acgtcggcat aaacggcggc aactcaggtg tacgcgaagg
    10561 tagctgggat ctgggggtag aatggttccc gatgcttgag aatcctgtta aaacgcagat
    10621 ttccgtcttt acatggacag atgccatcga ccatggtacg gaaatgaccg cgacccgtga
    10681 tggtgttcgt ggaaaagaaa aactggatgt ccccatcaag tttttatggt gctacgccag
    10741 taacacattg atcaatcaac atggcgacat caatcacacc catgaggtgc ttcaggatga
    10801 cagcaagtgc gagatgattg ttggcattga ccacttcatg acggcctcgg ctaagtattg
    10861 cgatatcctg ttgcccgacc tgatgccaac agagcaagaa gaccttatct ctcatgaatc
    10921 tgcagggaat atgggctatg tgatcctcgc ccaacccgca acctcagcaa aatttgaacg
    10981 caaacccatc tactggatgc tgagtgaagt cgccaaacgc ttaggaccag acgtttatca
    11041 aacctttact gaaggtcgca gtcagcatga atgggtcaaa tatctccatg cgaaaacgaa
    11101 ggaacgtaac cctgagatgc ccgactacga ggagatgaaa acgaccggga tctttaagaa
    11161 aaaatgcccg gaagaacact acgtcgcttt ccgcgcattc cgtgaagatc cacaggcaaa
    11221 cccgttgaaa acaccttcgg ggaaaatcga aatttattct gaacgactgg cgaagattgc
    11281 agatacctgg gaattgaaaa aagatgaaat tattcatccc cttcctgcgt ataccccagg
    11341 ttttgatggc tgggacgatc ccctgcggaa aacctatcca ctgcagttaa cgggcttcca
    11401 ttacaaagcg cgtacccact ccagctacgg caatattgat gtgttacagc aggcctgccc
    11461 acaagaggtg tggatcaacc ccattgatgc tcaggcacgc ggtatccgtc atggcgatac
    11521 cgtgcgggta tttaacaata atggagaaat gctgattgcc gcaaaagtga ctccgcgtat
    11581 tctgcctggc gttaccgcca tcgggcaagg tgcgtggctt aaagcggata tgtttggtga
    11641 ccgggtcgat cacggcggca gtatcaatat tctgacctct caccgccctt caccgctggc
    11701 aaagggaaac ccgtcgcaca gcaatcttgt ccagatcgaa aaggtttaag gagtagccca
    11761 tgaccacaca atatggattt tttatcgatt ccagccgctg tactggctgt aaaacctgcg
    11821 aactggcgtg caaagatttt aaagatcttg gcccggaagt cagtttccgc cgtatttatg
    11881 aatacgctgg cggcgactgg caggaagata atggcgtctg gcaccagaac gtgttcgctt
    11941 attatctctc catctcctgt aaccattgcg atgaccccgc atgtacaaaa gtctgcccca
    12001 gcggcgcaat gcataagcgt gaagatggct ttgtggtggt tgatgaagat gtttgtatcg
    12061 gctgccgcta ctgccacatg gcttgtccgt acggcgcacc acagtacaat gctgaaaaag
    12121 ggcacatgac gaagtgcgat ggttgttatt cgcgcgtcgc cgaggggaaa caacccatat
    12181 gcgtcgaatc ctgcccgctg cgtgcacttg agttcggtcc aattgaagag ctgcgccaga
    12241 agcacggcac tctggctgcc gtcgcgccac tgccgcgcgc gcatttcaca aaacccaata
    12301 tcgttatcaa acctaacgcc aacagccgcc cgaccggcga taccactggt tatctggcta
    12361 atccggagga agtgtaaaat gggaaatgga tggcatgaat ggccactggt tatctttacc
    12421 gtattgggtc aatgcgtagt aggcgcgctg attgttagcg gaattggctg gttcgccgcc
    12481 aaaaatgacg cagaccgtca gcgtattgtc cgtggcatgt ttttcctctg gctgttgatg
    12541 ggcatcgggt ttattgcctc tgtcatgcac ctgggatcgc cactgcgggc gtttaactcg
    12601 ctgaatcgaa tcggagcttc tggtctgagt aacgagattg ctgccggttc catatttttt
    12661 gccgttggcg gactatggtg gctggtggcg gtcatcggta aaatgccgca agcgttgggg
    12721 aaactctggt tacttgtcag catggcgctc ggcgtcattt tcgtctggat gatgacctgc
    12781 gtgtatcaaa tcgacactgt gccaacctgg cataacggtt acactacgct ggctttcttc
    12841 ctgacggtat tgttgagcgg tccaatactt gctgccgcaa ttctgcgggc agcccgcgtt
    12901 acatttaata ccacaccatt tgccatcatt agcgttctgg cgctgatcgc ttgtgccggg
    12961 gtgattgtgc tgcaaggttt gtctctggcg tctattcact catccgtgca acaagccagc
    13021 gcactggtac cagattacgc ctccttacag gtctggcgtg tggtattgtt atgcgcaggt
    13081 cttggatgct ggctttgccc gctcattcgt cgccgtgaac ctcacgttgc cggacttatt
    13141 ctggggctaa tcctcatact tggcggtgag atgatcgggc gtgtgctctt ttatggcctg
    13201 cacatgacag taggaatggc tattgcaggt taacaaattg gtgcggggca acccgcacat
    13261 ttcaggatgt taggaatgac ccatttttca cagcaagata atttttctgt cgcggcgcgc
    13321 gtgttgggcg cgctgtttta ttacgctcca gagagtgcgg aagccgcacc tctggttgcg
    13381 gtactcacca gtgacggctg ggaaactcag tggcctctac cagaagcgtc attagcgccg
    13441 ctggtgacgg cttttcagac acagtgtgaa gagactcacg cccaggcctg gcagcgtttg
    13501 tttgtcggcc cgtgggcact gccgtctccg ccgtgggggt cggtctggct ggatcgcgaa
    13561 tctgtgctgt ttggcgattc aacattggca cttcgtcagt ggatgcgcga gaaaggcatt
    13621 cagtttgaaa tgaagcaaaa cgaaccggaa gatcattttg gttcactgct gttgatggct
    13681 gcgtggttgg cggagaatgg tcgccagaca gaatgcgaag aattgctggc atggcacctt
    13741 tttccgtggt caacacgttt tcttgatgtt tttatcgaaa aagcagaaca ccctttctac
    13801 cgtgcactgg gtgaactggc tcgcttaacg ctggcgcaat ggcaatcaca actgttaatt
    13861 cctgtcgcgg ttaaaccgct gtttcgatag gatcaccgta atattgccgg atggtgacga
    13921 ttacgcgcaa tccggcaata ataggttaca gtgtcacgtt tttttatctc ttaaagcacg
    13981 cactgctttt gcggctggcc tcttttgccg caaaatagtc gcccgtgttt cattgcccat
    14041 ttctgctcat gcatcatcta cacatctatc cggatctgcg cactatgttc cgccgtctgc
    14101 ttatcgcaac agtcgtcggt attctcgcgg cctttgccgt tgccgggttt cgtcatgcga
    14161 tgctgctact ggagtggttg ttcctcaata atgactccgg cagtctggtc aatgcagcga
    14221 caaacctttc cccctggcga cggttgctaa ctccggcgct cggcggactg gcggcgggtt
    14281 tgttgctgat gggctggcag aaatttaccc aacaacgccc tcatgcgccg accgattaca
    14341 tggaagcgtt gcaaaccgat ggacagttcg attacgcagc aagcctggtt aaatcgcttg
    14401 cctctctgct ggtagtaacc agcggcagtg caattggtcg cgaaggtgcg atgattcttt
    14461 tagctgccct tgccgcctcc tgttttgccc aacgttttac gccacgccag gagtggaaat
    14521 tatggatcgc ctgtggggcc gcggcgggaa tggctgcggc ctatcgtgcc ccgcttgctg
    14581 gcagtttatt tatagccgaa gtgctgtttg gcactatgat gttggcctct ctcggcccgg
    14641 tgattatttc cgccgtcgtg gcattgctgg ttagcaatct gattaatcat agcgacgcgt
    14701 tactctacaa cgtacaactc tcagtgacgg ttcaggctcg tgactatgcg ctgattatca
    14761 gtacaggtgt gctggcaggt ctgtgcggac cactgttgtt aacgttaatg aacgcctgtc
    14821 atcgtggatt tgtgagtctc aaacttgcgc cgccctggca actggcacta ggcgggttga
    14881 tcgtgggtct gctttccctg ttcacacctg cagtgtgggg caacggctat agcaccgtac
    14941 aatccttttt aaccgcccca ccactgttaa tgatcattgc cgggatcttc ctttgtaaac
    15001 tgtgtgccgt gctggcgagt agcggttccg gcgcacccgg tggtgtcttt acaccgacgc
    15061 tatttatcgg tcttgccatt ggcatgttgt atggtcgtag cctgggatta tggttccctg
    15121 atggcgaaga aattacgctt ttactcggat tgaccgggat ggcgacactg ttggcggcaa
    15181 ccacgcacgc gccgattatg tcgacgttga tgatatgtga aatgaccggg gagtatcagc
    15241 tactccccgg tttattgatt gcctgcgtca ttgcgtcggt aatttcgcgg acgttacacc
    15301 gtgactctat ctaccgccag cacactgcgc agcatagcta agcggatgta ttgccccagt
    15361 tctcgctgtt cggcgcgcgg cagataaggc agttcaccaa tgagcggtgc cggaagtttt
    15421 ttacccagca catcaatgat ttccgcataa tgcgccagtc ctgggttgat tcggttagcc
    15481 acccagccaa tgagcggcag cccgtcgttg gcaatcgcct gagctgttag cagtgcatgg
    15541 ttaatgcaac cttcctgaat accgacaacc atcaacaccg gcaactgttc ctgcaccacc
    15601 cattcagaga gtggacgcaa atcattcatc agactgcgcc agccgccagt cccttctacc
    15661 acgacatgat cgactttatc ggtcaggttt gccaggccgt ttgaaatgag ggtgtaattg
    15721 attgggcaac tgtgcgccac gctactttct tcttcgctta acgcgatagg attaactgct
    15781 tcataaggca gttcgatggt tgaaacactc tgcaacacca gggcatcttt attacgcagc
    15841 ccttcgggtg tctctttgct ccccttcgct acgggtttat atcccgcaac cgtttttccc
    15901 tgggaggcta acgcttgtag caatgcgcgg gaaaccaccg ttttccctac agaagtgtct
    15961 gtaccggtaa taaagaaacg cttcagcatc actaactcca ccgttatgct tcacaaatat
    16021 aaaccaggaa aataattaac cttgaaagtc taagttatgc tttcctggcc caaattgaga
    16081 tagcgcaaat tttggtagaa cagttaaaaa atgttaaccc tgcaacagac gaatcaacaa
    16141 agaaccgtta tacatcgcgt cttttaccag tgcagcgcct gccatcgtgc cctggttaga
    16201 aaactgagta ctctcaacgc tgatgtgctg actatacgca ggaagggcct gctgacggat
    16261 gctgtctgag atgaccggga agaggatatc tgccgcttta cttaacggtg agccaatcag
    16321 tattttttgt gggttaaata aattcaccat gatggcaaga atgcgcccta catgcgcgcc
    16381 caccccggta atgatgtctt ttgccagcag atcgccgcgc aatgccgcct gacacaatga
    16441 gtccacggtt aacggttgtc catgtaacat cgagctcatg gattgattaa gacgcagctg
    16501 tgccagctca agaatactgt ccacactggc gatggtttcg aggcagccgt gattcccgca
    16561 ataacagcgt ttcccatacg ggtcgacctg tgtgtggcct atttccacga gactactgct
    16621 gcctgcgtgt agcagatgac catcggtaat gacgcccgcc cccacgttgt gatcgataac
    16681 cacctgaatc acatcgcgcg ccccgcgtga ggcaccaaac aaggcctctg ccatcgtcca
    16741 tgcgctgata tcatgctgaa tataaaccgg tacgccggta tgctgctcca gcgcctcgcc
    16801 gagcggcatc tcttttacat cctcgtagaa cggcatgcga tgtacaatac cattttccgt
    16861 atcaataatt cccggcaagg ttatggcaat cgaagttaga cgctcaagtt ttttctggtg
    16921 gcgaataaaa aactgatcga tatgggaaat gatacgatcc agcaatggcg agtcatcttt
    16981 taacgccagt tcctgcgact cttccaccac cagtttgctg ctcagatcgc gcagagcaag
    17041 gaaaatctcc ccgcgactaa tgcgcagaga aagatagtgc caggcttcag tttcaaccac
    17101 cagccccacc gccggacggc cacggttccc cgcttctttg atttccagct cttgcaccag
    17161 gtgtgcttcg agcatctcac ggacaatttt agtgatactg gcaggagcca gctgcgccag
    17221 acgggaaaga tcgatacgcg agactggacc aagctgatca atcaggcgat aaaccgcgcc
    17281 cgcgttggtc tgctttattt gatcaatgtg cccaggctgg ttttcagcaa ccaccgcata
    17341 ctccctatat tttcgcgctc cgaaataatc tgtaggctat ggtgaagcac ttcaatacgt
    17401 gtcgtcaaat ttttacttag gcatgtgatt aacagcacat ttttcgggct ttttcgctga
    17461 aatttccctc acctgagagc attcagcaga tgaatacgaa agttacgcgc agccggactt
    17521 tgttcatgat gtttcggcca gaccaaccac atttcagaaa ccgcatcctc ttcagcaatc
    17581 ggcacccagc gcatttcgtt gagctgaaca cgtttaaatg aagcaggcaa aattgaaaca
    17641 cccagaccgg cggaaaccag accgatgatg gtcattgcct cgcccacctc ctgagtgatg
    17701 acgggcgtca aatggtaacg tcgcatcagc ccgagaatat cgtcatacag ccctgtcccg
    17761 acgtgcggat caaaaaagac gaagggttct ttcgccagtt cagccagcgt tacattcggg
    17821 ttatttgcca ggggatgatc gtgcgggatc atcgccataa gcggttcatg gacgatgact
    17881 gcgtgttcaa gcgtctccgg taacgctgtg taacgcagca atcccatatc cagcgttcct
    17941 tcaatgagcg gagcgatttg ctcacgagtg ttcatttcgc gggtttgtaa atggacatcg
    18001 ggataatcac ggcgaaacag cgataacgta tcggacaccg cccgaataaa aggagcggac
    18061 gaagtaaaac caatgcgcaa ctcccccgct tccccctgat gcagtctttc agcgcgagcg
    18121 gcagcgtcat ctaccataga caggatttgc cgactatctg caagaaactg ttttcctgct
    18181 gccgtcagca atacactgcg attagttcgt gccagcagtc gggcaccaat ttgttgctcc
    18241 agtgcctgaa tctgctgact tagcggtggt tgcgaaatat tcaggcgggc agcggcgcgc
    18301 ccgaaatgca gctcttccgc aacagcaaca aagtaacgca gatgacgaag ttcaatattc
    18361 atatttaaaa catcttattt gagattatta atatattaga cagaacaatt cgattttcct
    18421 aacctatgta taagcctgat ccacaggcat atttagcaag gatttcaagt gagccgtact
    18481 acaactgttg atggcgctcc ggcaagcgac actgacaagc aaagcatttc tcagccaaat
    18541 caatttatta aacgcggtac gccgcaattt atgcgcgtca ccctggcgct gttctctgcc
    18601 ggactggcaa catttgcact tctctattgt gtgcagccta tccttccggt gctttcgcag
    18661 gagtttggct taacgcccgc gaacagtagt atttcactgt ccatttccac ggcgatgttg
    18721 gctattggtt tgctgtttac tggcccccta tccgatgcca ttggtcgcaa accagtgatg
    18781 gtcacggcgc tactgttggc ctccatttgt acgttacttt cgacaatgat gaccagctgg
    18841 cacggcattt tgattatgcg cgccttgatt gggctttcgt taagtggcgt ggcagctgtt
    18901 ggcatgactt atcttagcga ggaaatccac cccagtttcg tggccttttc gatggggttg
    18961 tatatcagcg gcaactcaat tggcggcatg agcggacgct taattagtgg tgtcttcacg
    19021 gactttttca actggcgaat tgctctggcg gcaatcggtt gtttcgcgct agcctcggcg
    19081 ttgatgttct ggaaaatcct ccctgaatca cgccattttc gcccgacttc gctgcgacct
    19141 aaaacgttgt ttatcaactt tcgtctgcac tggcgtgacc ggggattacc gttattgttc
    19201 gcagaaggct ttttgctgat ggggtcgttc gtcacgctgt ttaattacat cggatatcgg
    19261 ttgatgctct ccccctggca tgtcagtcag gccgtggttg gcttattatc gctggcttat
    19321 ttgaccggta catggagctc acccaaagcc ggaaccatga ccacccgcta cgggcgcggt
    19381 ccggtgatgt tgttttcgac gggggttatg ctgtttggtt tactgatgac cttattcagc
    19441 tcgctgtggc tgatctttgc cggaatgtta ctcttctcag caggattctt cgcagcccac
    19501 tcagttgcca gcagctggat tggcccccgc gcaaaacgcg ctaaaggcca ggcctcctcg
    19561 ctgtatctgt tcagttacta tctggggtcg agtattgccg ggacgctggg tggtgttttc
    19621 tggcataact atggctggaa cggcgtcggc gcatttattg ctctgatgct ggtcattgct
    19681 ctgctggtcg ggacgcgttt gcatcgtcgt ctgcacgcct gaaaaataag cccggactgc
    19741 ggtaactaca cgtccggact tattgcttgc tcaaaccaac gttaatagcc atcctaaaat
    19801 agacgaagcg ccagccaatt cccgcagcgc gtctagcgtc atcaggatta taagtaccca
    19861 aataaacgga ttcattttgc tgtgtgtcat ttattactga tgcgcagtta ttctaccgct
    19921 ttgtaagtag taaaatagtt aacccgatca agactactat tattggtagc taaatttccc
    19981 ttaagtcaca atacgttatt atcaacgctg taatttattc agcgtttgta catatcgtta
    20041 cacgctgaaa ccaaccactc acggaagtct gccattccca gggatatagt tatttcaacg
    20101 gccccgcagt ggggttaaat gaaaaaacaa attgagggta tgacaatgaa aaaagtatta
    20161 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
    20221 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
    20281 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
    20341 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
    20401 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
    20461 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc aggtaacgtt
    20521 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
    20581 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
    20641 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
    20701 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
    20761 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
    20821 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
    20881 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
    20941 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acgccgccta
    21001 agggtaaagc ggataaggcg gtagcgctgc gttttgtgtc aaataaaggt ctttggcgct
    21061 atgacatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
    21121 atggggatgg ttggattgtt cctcccgcag ctgcaccatg ggacttcggc ttgattgtgc
    21181 tgcgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
    21241 ttactgccgc attaaaatcg gctggtcgta aagtgactca ggcgggatat cctgaagatc
    21301 atctcgatac gttatacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
    21361 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctttg atgttgcata
    21421 ccgatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
    21481 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
    21541 cgcaaaaata atgttcaaac gctgcccgac agtgcgggca gcgtgttcat caggcaagtt
    21601 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
    21661 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
    21721 atgcataagc gacagacaag tcgatccctt ttacggcctg agaaagcgca ctaaaggcag
    21781 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
    21841 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
    21901 cccattcaaa ctgcgccatg gttcacttcc agttcaggtt tacgcgcttt acgggtacct
    21961 gatttgatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
    22021 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
    22081 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
    22141 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
    22201 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
    22261 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
    22321 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
    22381 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
    22441 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
    22501 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
    22561 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
    22621 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
    22681 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
    22741 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
    22801 cattatttat tgctgttata tataacttat ttcacataac acgttgattt aattaaataa
    22861 ttttcaccgc aaaaagtgcc aaaaatcgca aagtgactac accagt
//
LOCUS       DAZGLJ010000053        22856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000053 DAZGLJ010000000
VERSION     DAZGLJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.053"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            63..1268
                     /gene="yedE"
                     /locus_tag="ACHK49_001893"
     CDS             63..1268
                     /gene="yedE"
                     /locus_tag="ACHK49_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO9262549.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            1265..1498
                     /gene="yedF"
                     /locus_tag="ACHK49_001894"
     CDS             1265..1498
                     /gene="yedF"
                     /locus_tag="ACHK49_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO9262550.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            1605..2273
                     /gene="yedK"
                     /locus_tag="ACHK49_001895"
     CDS             1605..2273
                     /gene="yedK"
                     /locus_tag="ACHK49_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO9262551.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNIKNQGAEL
                     IQPV"
     gene            complement(2668..2982)
                     /gene="fliE"
                     /locus_tag="ACHK49_001896"
     CDS             complement(2668..2982)
                     /gene="fliE"
                     /locus_tag="ACHK49_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO9262552.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHATLDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            3197..4855
                     /gene="fliF"
                     /locus_tag="ACHK49_001897"
     CDS             3197..4855
                     /gene="fliF"
                     /locus_tag="ACHK49_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO9262553.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4848..5843
                     /gene="fliG"
                     /locus_tag="ACHK49_001898"
     CDS             4848..5843
                     /gene="fliG"
                     /locus_tag="ACHK49_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO9262554.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5836..6522
                     /gene="fliH"
                     /locus_tag="ACHK49_001899"
     CDS             5836..6522
                     /gene="fliH"
                     /locus_tag="ACHK49_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO9262555.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQMPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            6522..7895
                     /gene="fliI"
                     /locus_tag="ACHK49_001900"
     CDS             6522..7895
                     /gene="fliI"
                     /locus_tag="ACHK49_001900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO9262556.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7914..8357
                     /gene="fliJ"
                     /locus_tag="ACHK49_001901"
     CDS             7914..8357
                     /gene="fliJ"
                     /locus_tag="ACHK49_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO9262557.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            8354..9481
                     /gene="fliK"
                     /locus_tag="ACHK49_001902"
     CDS             8354..9481
                     /gene="fliK"
                     /locus_tag="ACHK49_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO9262558.1"
                     /translation="MIRLAPLITADVDNATLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAVPVLSAPLGSHEWQQSLSQHISLFTRQRQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLLVPVSIQGRVTGNSGVDIFA"
     gene            9586..10050
                     /gene="fliL"
                     /locus_tag="ACHK49_001903"
     CDS             9586..10050
                     /gene="fliL"
                     /locus_tag="ACHK49_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO9262559.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            10055..11059
                     /gene="fliM"
                     /locus_tag="ACHK49_001904"
     CDS             10055..11059
                     /gene="fliM"
                     /locus_tag="ACHK49_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO9262560.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            11056..11469
                     /gene="fliN"
                     /locus_tag="ACHK49_001905"
     CDS             11056..11469
                     /gene="fliN"
                     /locus_tag="ACHK49_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO9262561.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            11472..11837
                     /gene="fliO"
                     /locus_tag="ACHK49_001906"
     CDS             11472..11837
                     /gene="fliO"
                     /locus_tag="ACHK49_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO9262562.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            11837..12574
                     /gene="fliP"
                     /locus_tag="ACHK49_001907"
     CDS             11837..12574
                     /gene="fliP"
                     /locus_tag="ACHK49_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO9262563.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            12584..12853
                     /gene="fliQ"
                     /locus_tag="ACHK49_001908"
     CDS             12584..12853
                     /gene="fliQ"
                     /locus_tag="ACHK49_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO9262564.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12862..13647
                     /gene="fliR"
                     /locus_tag="ACHK49_001909"
     CDS             12862..13647
                     /gene="fliR"
                     /locus_tag="ACHK49_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO9262565.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLADTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLILTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            13937..14560
                     /gene="rcsA"
                     /locus_tag="ACHK49_001910"
     CDS             13937..14560
                     /gene="rcsA"
                     /locus_tag="ACHK49_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO9262566.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(14604..14792)
                     /gene="dsrB"
                     /locus_tag="ACHK49_001911"
     CDS             complement(14604..14792)
                     /gene="dsrB"
                     /locus_tag="ACHK49_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO9262567.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14955..15182
                     /gene="yodD"
                     /locus_tag="ACHK49_001912"
     CDS             14955..15182
                     /gene="yodD"
                     /locus_tag="ACHK49_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO9262568.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            15480..16295
                     /gene="yedP"
                     /locus_tag="ACHK49_001913"
     CDS             15480..16295
                     /gene="yedP"
                     /locus_tag="ACHK49_001913"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO9262569.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQSSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAH"
     gene            complement(16292..17986)
                     /gene="dgcQ"
                     /locus_tag="ACHK49_001914"
     CDS             complement(16292..17986)
                     /gene="dgcQ"
                     /locus_tag="ACHK49_001914"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO9262570.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFVGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLHWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(18224..18406)
                     /gene="yodC"
                     /locus_tag="ACHK49_001915"
     CDS             complement(18224..18406)
                     /gene="yodC"
                     /locus_tag="ACHK49_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO9262571.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(18485..19402)
                     /gene="yedI"
                     /locus_tag="ACHK49_001916"
     CDS             complement(18485..19402)
                     /gene="yedI"
                     /locus_tag="ACHK49_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO9262572.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVAGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            19575..20495
                     /gene="yedA"
                     /locus_tag="ACHK49_001917"
     CDS             19575..20495
                     /gene="yedA"
                     /locus_tag="ACHK49_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO9262573.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIPDASSE"
     gene            complement(20484..20954)
                     /gene="vsr"
                     /locus_tag="ACHK49_001918"
     CDS             complement(20484..20954)
                     /gene="vsr"
                     /locus_tag="ACHK49_001918"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO9262574.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVLDEFRCVIFAHGCFWHHHHCYLFKVPATRTDFWLEKIGKNVERDRRDIS
                     HLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(20935..22353)
                     /gene="dcm"
                     /locus_tag="ACHK49_001919"
     CDS             complement(20935..22353)
                     /gene="dcm"
                     /locus_tag="ACHK49_001919"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO9262575.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISKCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(22420..>22856)
                     /locus_tag="ACHK49_001920"
     CDS             complement(22420..>22856)
                     /locus_tag="ACHK49_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFO9262576.1"
                     /translation="EFVQFPAEKIEAVCHAIAAHSFSAQIAPLTTEAKIVQDADRLEA
                     LGAIGLARVFAVSGALGLALFDGEDPFAQHRPLDDKRYALDHFQTKLLKLPQTMQTAR
                     GKQLAQHNAQFLVEFMAKLGAELAGENEGIDHKVIDAFSPAG"
BASE COUNT         5438 a         6030 c         5956 g         5432 t
ORIGIN      
        1 atttgcatga tgtatgattc gcgattcttg tttcaaacct tctgagttca gaggctacat
       61 tcatgtcatg gcagcaattc aaacacgcct ggttgattaa attctgggca cccatccctg
      121 cggtcatcgc ggcgggtatt ctctccactt actattttgg cattactggc accttttggg
      181 ctgtcacggg tgaatttacc cgttggggcg gccagctcct gcagctgttt ggcgtccatg
      241 ctgaagagtg gggttatttt aaaattatcc atctggaagg atcgccatta acccgcatcg
      301 acgggatgat gatcctcggc atgtttggcg gctgctttgc cgcagcgctg tgggccaaca
      361 atgtcaaact gcgcatgcca cgcagccgta tccgcattat gcaggccatt attggcggta
      421 ttatcgctgg ttttggcgcg cgtctggcaa tgggctgtaa cctggcggcg ttctttaccg
      481 gtattcctca gttctcgctg catgcctggt tctttgccat cgccactgcc attggttcat
      541 ggtttggcgc acgctttacc ctgctgccca tcttccgtat tcccgtgaaa atgcagaaag
      601 tttctgccgc ctcaccgctg acgcaaaaac cggatcaggc gcggcgtcgt tttcgtctcg
      661 ggatgctggt ctttttcggt atgctgggct gggcgctgct cacggcgatg aaccaaccaa
      721 aactggggct ggcaatgctg tttggcgtcg gctttggttt actgattgaa cgtgcgcaaa
      781 tctgctttac ttcggcgttc cgcgatatgt ggatcaccgg acgtacccat atggcgaaag
      841 caatcattat cggtatggcg gtgagtgcca tcgggatctt cagttacgta cagttaggcg
      901 ttgaacccaa aatcatgtgg gcgggaccaa acgcggtaat tggtggttta ctgtttggtt
      961 ttggcatcgt gctggcaggc ggctgcgaaa ccggctggat gtaccgcgcg gtagaaggcc
     1021 aggtgcacta ctggtgggtc ggtctgggca atgtgatcgg ctcgacgatt ctggcgtatt
     1081 actgggatga tttcgctccg gcgctggcca ccgactggga caaaatcaac ctgctgaaaa
     1141 cctttggccc gatgggtggc ctgctggtga catatttgct gttgtttact gcgctgatgt
     1201 tgattatcgg ctgggaaaaa cgcttcttcc gccgtgcggc accgcagact gctaaggaga
     1261 tcgcatgaaa aatatcgttc ctgattaccg ccttgatatg gtgggtgaac cctgccctta
     1321 tccggcagtc gcaactcttg aggcgatgcc gcagttaaaa aaaggggaaa tcctggaagt
     1381 ggtgagcgac tgtccacagt cgatcaataa tattccactg gatgcgcgta atcacggcta
     1441 tacggtgctg gatattcagc aagacgggcc gaccattcgt tatttaattc agaagtaatc
     1501 cctcattccc gctgtggccc acagcgggaa catcgttgcc tctgacacat agattttacc
     1561 cgccccctct cctattcttt tcagtatgat ctcaggaggt ttctatgtgt ggacgctttg
     1621 cccaatccca aacgcgtgaa gattaccttg cgcttctcgc ggaagatatt gaacgcgata
     1681 ttccctacga tcccgaaccc attggcagat acaacgtcgc gccgggaacc aaagttcttc
     1741 tgctcagtga acgtgatgaa caccttcatc tggatccggt tttctgggga tatgctcccg
     1801 gatggtggga taaaccgccg ctgattaacg cccgcgtaga aactgcggcc accagtcgta
     1861 tgtttaaacc gctctggcaa catggtcggg caatctgttt tgccgatggc tggtttgagt
     1921 ggaaaaaaga aggcgacaaa aaacagcctt attttatcta tcgcgctgat ggacaacctg
     1981 tttttatggc cgcgataggt agcacaccgt ttgagcgcgg tgacgaagcc gaaggatttt
     2041 taattgtcac tgcagcagca gaccagggtc tggtagatat tcatgaccgc cgcccgctgg
     2101 ttctgtcgcc agaagctgca caggaatgga tgcggcaaga gattggcggt aaagaagcat
     2161 cagaaattgc taccaacggc tgtgtgccag caaaccagtt cacctggcac ccggtatcgc
     2221 gcgcggtggg taatatcaaa aaccagggag cggagttaat tcaaccggtt tgataagcca
     2281 ggagactatt gatcaataat actgcgtcat aagaaatctc tattagacaa tagtagacgc
     2341 tccttccaca tgcgccacaa gaaacctcta ttccagtgac acaattacgc ctgattattt
     2401 ccctagcccg gaatatgaat aaaaacatca acaaagatta aattattcaa acgttactgt
     2461 tgcgtctgcg gtcgtagcaa catccgacgt ggtgatgagt gttaaaagag acactctgat
     2521 gttcagaggg attatcgcgt ttacggatcg tggatggcag aaaactttca ggatcaggta
     2581 tccattttaa accagaaatt gagtgagttt gccccatcca tgccccaagc ggtgagattg
     2641 gaagtaatta acaacaggtt acaaaaccta cacctgcatg gacatcactt cctgatacgc
     2701 cgccaccagc ttattacgca cctgaatccc catttgcata gaaactgagg ctttttgcat
     2761 atcggtcatc acatcgttta acgccacgcc gggttcaccg agggtgaatt tttctgcctg
     2821 cgtgcgggca gctgtttgtg tatcgctaat gcgatcgagc gtggcgtgca gctgcccggc
     2881 aaaactaatg gtcggttgcg gcagtgaatc ctgcgcacgc gcactcatcg ccgtagcctg
     2941 taactggctg ataacccctt caatcccctg tatcgctgac attttcgtct cctggataat
     3001 ttctggtagc aaagcctacc agtaagtcaa taagataaag gcgctaaata acaacaaaaa
     3061 aacgggttta ttggcggata gaaaaaaacg aaagcacaaa taatggcagc gtcaattttt
     3121 cgagtttgct gacccgggag tgagtcttgt tccactttgc caataacgcc gtccataatc
     3181 agccacgagg tgcgcgatga atgcgactgc agcccagaca aaatctcttg agtggcttaa
     3241 tcgcctgcgt gcgaatccga aaattccatt gattgttgcc ggttccgcgg cagtggcggt
     3301 catggtcgca ctgatcctgt gggcgaaagc ccccgactac cgcacattat tcagcaatct
     3361 ttccgatcag gatggtggcg caattgtcag ccaactgacg caaatgaata ttccttaccg
     3421 cttcagcgaa gccagcggcg ctattgaagt tccggcagat aaagttcacg aactgcgtct
     3481 gcgcctggca caacaaggtt tgcccaaagg cggcgcggtc ggtttcgaac tactcgatca
     3541 ggaaaagttt ggtatcagcc agttcagcga acaggtgaat tatcagcggg cgctggaagg
     3601 cgagctttct cgtaccatcg aaactatcgg cccggtaaaa ggggcgcgcg tacatctggc
     3661 aatgccgaaa ccgtctttat tcgtccgtga acaaaaatcc ccttctgcat cggtgacggt
     3721 aaatctgtta cccggccgcg cactcgatga agggcaaatt agcgccattg tgcatctggt
     3781 ttccagcgcc gttgctggtc tgccgccggg aaacgtcacg ctggtggatc agggcggaca
     3841 tctgttaacc cagtccaata ccagcgggcg cgatcttaat gatgctcagt tgaaatatgc
     3901 cagcgatgtc gaaggccgta ttcagcggcg tattgaagca atcctgtcgc ctattgttgg
     3961 taacggtaat attcacgccc aggtcacggc gcagctggac ttcgccagta aagaacaaac
     4021 ggaagaacag tatcgcccta acggtgatga atctcatgcg gcgcttcgtt cacgccagct
     4081 taatgagagc gagcaaagcg gttccggtta tccgggcggc gtaccggggg cgttgtcgaa
     4141 tcaaccggca cctgcgaata acgcgccaat cagcacgcct ccggcaaatc aaaataaccg
     4201 ccagcagcag gcgagcacca ccagcaatag cgggccgcgt agcacacagc ggaatgaaac
     4261 cagtaactac gaagtcgatc gcaccattcg tcataccaaa atgaacgtgg gcgatgtgca
     4321 acgtctgtca gtcgcggtag tggtgaatta caaaaccttg ccagacggta aaccattgcc
     4381 tctcagcaac gaacagatga agcaaattga agatctgacc cgcgaggcga tgggcttttc
     4441 tgaaaaacgc ggtgactcgc tcaatgtcgt taactcgccg ttcaatagca gtgacgaaag
     4501 cggcggagaa ctgccgttct ggcaacagca agcgtttatc gatcagttgc tggctgccgg
     4561 tcgctggttg ctggtactgc tggtggcgtg gctgctgtgg cggaaagcgg tacgcccgca
     4621 gttaacacgt cgcgccgagg cgatgaaagc tgtacagcaa caggcgcagg cccgtgagga
     4681 agtggaagat gcggtggaag tccgcctgag caaagacgaa caactccaac aacgacgcgc
     4741 taaccaacgt ctgggggcag aagtcatgag ccagcgtatc cgtgaaatgt ctgataacga
     4801 tccgcgcgtg gtggcgctgg tcattcgcca gtggataaat aacgatcatg agtaacctga
     4861 caggcaccga taaaagcgtc atcctgctga tgaccattgg cgaagaccgg gcggcagagg
     4921 tgttcaagca cctctcccag cgcgaagtgc aaaccctgag cgctgcaatg gcgaacgtca
     4981 cgcagatctc caacaagcag ctaaccgatg tgctggcgga gtttgagcaa gaagctgaac
     5041 agtttgccgc actgaatatc aacgccaacg attatctgcg ttcggtattg gtcaaagctc
     5101 tgggtgagga acgtgccgcc agcctgctgg aagatattct cgaaactcgc gataccgcca
     5161 gcggtattga aacgctcaac tttatggagc cgcagagtgc cgccgatctg attcgcgatg
     5221 agcatccgca aattatcgcc accattctgg tgcatctgaa gcgcgcccag gccgccgata
     5281 ttctggcgtt gttcgatgaa cgtctgcgcc acgacgtgat gttgcgtatc gccacctttg
     5341 gcggcgtgca gccagccgcg ctggcggaac tgaccgaagt actgaatggc ttgctcgacg
     5401 gtcagaatct caagcgcagc aaaatgggcg gcgtgagaac ggcagccgaa attatcaacc
     5461 tgatgaaaac tcagcaggaa gaagccgtta ttaccgccgt gcgcgaattc gacggcgagc
     5521 tggcgcaaaa aattatcgac gagatgttcc tgttcgaaaa tctggtggat gtcgacgatc
     5581 gcagcattca gcgtctgttg caggaagtgg attccgaatc gctgttgatc gcactgaaag
     5641 gggccgagca gccactgcgc gagaaattcc tgcgcaatat gtcgcagcgt gccgccgata
     5701 ttctgcgcga cgatctcgcc aaccgtggtc cggtgcgtct gtcgcaggtg gaaaacgaac
     5761 agaaagcgat tctgctgatt gtgcgccgcc ttgccgaaac tggcgagatg gtaattggca
     5821 gcggcgagga tacctatgtc tgataatctg ccgtggaaaa cctggacgcc ggacgatctc
     5881 gcgccaccac aggcagagtt tgtgcccatg gtcgagccgg aagaaaccat cattgaagag
     5941 gccgaaccca gccttgagca gcaactggcg caactgcaaa tgcaggccca tgagcaaggt
     6001 tatcaggcgg gtattgccga aggtcgccag caaggtcata agcagggcta tcaggaagga
     6061 ctggcccagg ggctggagca aggtctggca gaggcgaagt ctcaacaagc gccaattcat
     6121 gcccggatgc agcaactggt cagcgaattt caaactaccc ttgatgcact tgatagtgtg
     6181 attgcttcgc gcctgatgca gatggcgctg gaggcggcac gtcaggtcat cggtcagatg
     6241 ccaacagtgg ataactcggc actgatcaaa cagatccaac agttgttgca gcaagaaccg
     6301 ttattcagcg gcaaaccgca actacgcgtg caccccgatg atctgcaacg tgtggatgat
     6361 atgctcggcg caaccttaag tttgcatggc tggcgcttgc ggggcgatcc cacactccat
     6421 cctggcggct gtaaagtctc cgccgatgaa ggcgatctcg acgccagtgt cgccactcgc
     6481 tggcaagaac tctgccgtct ggcagcacca ggagtggtgt aatgaccacg cgcctgactc
     6541 gctggctaac cacgctggat aactttgaag ccaaaatggc gcagttgcct gcggtacgtc
     6601 gctacgggcg attaactcgc gctaccgggc tggtgctgga agccaccgga ttacaattgc
     6661 cgctcggcgc aacctgtgtc attgagcgcc agaacggcag cgaaacgcac gaagtagaaa
     6721 gcgaagtcgt tggctttaac ggtcaacggc tgtttttaat gccgctggag gaagtcgaag
     6781 gtgtcctgcc cggcgcgcgt gtttatgcca aaaacatttc ggcagaaggg ctgcaaagcg
     6841 gcaagcagtt gccgctcggt ccggcgttat taggtcgtgt tctggacggc agcggtaaac
     6901 cgctcgatgg cctcccctcc cccgatacga cggaaaccgg tgcgctgatt accccaccat
     6961 ttaacccgtt gcaacgtaca ccgattgaac atgtgctgga caccggcgtg cgcccaatca
     7021 atgccctgct taccgttggg cgtgggcagc gtatggggct gtttgccggg tccggcgttg
     7081 gtaaaagtgt gctgctgggg atgatggcac gttacacccg cgccgatgtc attgtcgtgg
     7141 gtttgattgg tgaacgcgga cgcgaagtaa aagattttat tgagaatatc ctcggtgccg
     7201 aagggcgtgc acgctccgtg gtgattgccg ctccggcgga tgtctctccg cttctgcgaa
     7261 tgcaaggtgc cgcctatgcc acgcgaattg ccgaagattt tcgcgatcgt ggtcagcatg
     7321 tgttgctgat tatggactcc ctcacccgct acgcgatggc ccagcgtgaa attgcgctgg
     7381 cgattggcga accacctgcc actaaaggtt atccaccgtc ggtgtttgcc aaattaccgg
     7441 cactggtcga gcgtgccgga aatggaatta gcggcggcgg ctcgattacc gcgttttata
     7501 ccgtgctcac cgaaggcgat gaccagcagg accccattgc cgactctgcg cgggcaatcc
     7561 tcgacggtca cattgtgctg tctcgccgac tggcggaagc cgggcactat ccggctatcg
     7621 atattgaagc gtcgatcagc cgcgcaatga cggcgttgat cagtgagcaa cattacgcgc
     7681 gagtgcgcac cttcaaacag ctgttgtcga gttttcagcg taaccgcgat ctggttagcg
     7741 tcggcgcgta tgccaaaggc agcgatccga tgctcgacaa agccatcgcc ctgtggccgc
     7801 agctggaggg ctatttgcaa caaggcattt ttgaacgcgc ggactgggaa gcgtctctcc
     7861 aggggctgga gcgtattttc ccgacagtgt cataacccag gagataacgg cagatggcag
     7921 aacatggcgc gctggcgacc ctgaaagatc tggcagaaaa agaggtagag gatgccgcgc
     7981 gcctgctggg tgaaatgcgt cgcgggtgtc agcaggcgga agaacagctc aaaatgctga
     8041 tcgattatca gaatgaatat cgcaataacc tcaacagcga tatgagtgcc gggatgacca
     8101 gcaaccgctg gatcaactat cagcagttta tccagacgct ggaaaaagcc attactcagc
     8161 atcgccagca acttaatcag tggacgcaga aagttgacat tgccctgaac agctggcgag
     8221 aaaagaaaca acgtttgcag gcctggcaga cactgcagga acggcaatcc acggcggcac
     8281 tgcttgcaga aaaccgcctc gatcagaaaa agatggatga gttcgcccag cgcgccgcca
     8341 tgaggaaacc tgaatgattc gcttagcgcc cttaattacc gccgacgttg ataacgccac
     8401 attgcctggc ggcaaagcca gcgatgctgc acaagatttt ctcgcgttgt tgagcgaagc
     8461 attagcaggc gagacaacta ccgacaaagc ggccccccag ttgctggtgg caacagataa
     8521 gcccacgaca aaaggcgagc cgctggtcag cgagattctt gccgatgcac aacaagcgga
     8581 tttactgatc cctgtggatg aaacaccgcc tgtcatcaac gacgaacaat ccacatcaac
     8641 accattaacc accgctcaaa cgatgacgtt ggctgcggtg gctgacaaca atacggcaaa
     8701 agacgaaaaa gcggatgatc tgaatgaaga cgtcaccgca agcctgagcg ccctttttgc
     8761 gatgttgccg ggttttgaca atacgcccaa agtgactgat gtgccgtcaa ccgtgttacc
     8821 ggcagagaaa ccaacgctct tcacaaaact gacttctgcg caactcacaa cagcacagcc
     8881 tgatgatgcc cccggcacgc cagctcagcc attaacaccg ctggtagcag aagcccagag
     8941 taaagcggaa gtcatcagca caccttcacc ggtgaccgct gccgccagcc cgctaatcac
     9001 tccacaccag acacagccac tgcccaccgt cgccgtgcct gtgttgagtg caccgctggg
     9061 ttctcacgaa tggcaacaat cattaagcca gcatatttcg ctgttcaccc gccagaggca
     9121 acaaagtgca gagttgcgtc tgcacccgca ggatttaggt gaagtgcaaa tctccctcaa
     9181 agtggatgat aaccaggcac aaatccagat ggtttcaccg catcagcatg tacgcgccgc
     9241 gctggaagca gcgcttccgg tactgcgtac gcagctggcc gaaagtggca ttcagttagg
     9301 gcaaagcaac atcagtggcg aaagctttag tggtcagcag caggccgctt cccaacaaca
     9361 gcaaagccaa cgcacagtaa accatgaacc tctggcgggg gaagacgacg atacgcttct
     9421 ggttcccgtc tctatacaag ggcgcgtaac aggcaacagc ggcgttgata ttttcgccta
     9481 acgtcagagg tagcaccgta atccgcgtct tttccccgct ttgttgcgct caagacgcag
     9541 gataattagc cgataagcag tagcgacaca ggaagaccgc aacacatgac tgattacgcg
     9601 ataagcaaga aaagcaagcg atcgctttgg atcccgattc tggtattcat taccctcgcg
     9661 gcctgtgcca gcgcaggtta cagctactgg cattcgcatc aggttgccgc tgacgacaaa
     9721 gcgcagcaac gcgtcgtacc ctccccggtc ttctacgcgc tggatacctt cacggtcaat
     9781 ctgggcgatg cggatcgcgt gctttatatc ggcataaccc tgcgcctgaa agatgaagct
     9841 acccgctcgc ggctgagtga gtatttgccg gaagtccgta gccgtttgct gttactgttt
     9901 tcgcgtcagg atgctgccgt actggcgaca gaagaaggca agaaaaacct gattgccgag
     9961 attaaaacca cgctttccac cccgcttgtt gccgggcaac cgaaacagga tgtcaccgac
    10021 gtgctgtata ccgcttttat tctgcgataa cgacatgggc gatagtattc tttctcaagc
    10081 tgaaattgat gcgctgttga atggtgacag cgaagtcaaa gacgaaccga cagccagtgt
    10141 tagcggcgaa agtgacattc gtccgtacga tccgaatacc caacgacggg ttgtgcgcga
    10201 acgtttgcag gcgctggaaa tcattaatga gcgctttgcc cgccattttc gtatggggct
    10261 gttcaacctg ctgcgtcgta gcccggatat aaccgtcggg gccatccgca ttcagccgta
    10321 ccatgaattt gcccgcaacc tgccggtgcc gaccaacctg aaccttatcc atctgaaacc
    10381 gctgcgcggc acagggctgg tggtgttttc accgagtctg gtgtttatcg ccgtggataa
    10441 cctgtttggc ggcgatggac gcttcccgac caaagtggaa ggtcgcgagt ttacccatac
    10501 cgaacagcgc gtcatcaacc gcatgttaaa gctggcgctt gaaggctata gcgacgcctg
    10561 gaaggcgatt aatccgctgg aagttgagta cgtgcgttcg gaaatgcagg tgaaatttac
    10621 caatatcacc acctcgccga acgacattgt ggttaacacg ccgttccatg tggagattgg
    10681 caacctgacc ggcgaattta atatctgcct gcctttcagc atgatcgagc cgctacggga
    10741 attgttggtt aacccgccgc tggaaaactc gcgtaatgaa gatcagaact ggcgcgataa
    10801 cctggtgcgc caggtgcagc attcacagct ggagctggtc gccaactttg ccgatatctc
    10861 gctacgcctg tcgcagattt taaaactgaa acccggtgac gtcctgccga tagaaaaacc
    10921 cgaccgcatc atcgcccatg ttgacggcgt cccggtgctg accagtcagt acggcactct
    10981 caacggtcag tatgcgttac ggatagaaca tttgattaac ccgattttaa attctctgaa
    11041 cgaggaacag cccaaatgag tgacatgaat aatccggccg atgacaacaa cggcgcaatg
    11101 gacgatctgt gggctgaagc tttgagcgaa caaaaatcaa ccagcagcaa aagcgctgcc
    11161 gacgcggtgt tccagcaatt tggcggtggt gatgtcagcg gaacgttgca ggatatcgac
    11221 ctgattatgg atattccggt caagctgacc gtcgagctgg gccgtacgcg gatgaccatc
    11281 aaagagctgt tgcgtctgac gcaagggtcc gtcgtggcgc tggacggtct ggcgggcgaa
    11341 ccactggata ttctgatcaa cggttattta attgcccagg gcgaagtggt ggtcgttgcc
    11401 gataaatatg gcgtgcggat caccgatatc attactccgt ctgagcgaat gcgccgcctg
    11461 agccgttagt gatgaataac cacgctactg tgcaatcttc cgcgccggtt tctgctgcgc
    11521 cactgctgca ggtgagcggc gcactcatcg ccattattgc cctgatcctc gctgctgcct
    11581 ggctggtaaa acggttggga tttgtcccta aacgcactgg cgttaacggt ctgaaaatta
    11641 gcgccagtgc ttcgctgggc gcgcgtgaac gggttgtggt ggtcgatgtg gaagatgcac
    11701 ggctggtgct cggcgttacc gcagggcaaa tcaatctgct gcataaactt cccccttcag
    11761 caccaacgga agagataccg cagcccgatt ttcagtcggt tatgaaaaac ttgcttaagc
    11821 gtagcgggag atcctgatgc gtcgtttgtt gtctgtcgca cctgtccttc tctggctggt
    11881 tacgcccctc gccttcgcgc aactgccggg tatcaccagc cagccgctgc ctggcggtgg
    11941 acaaagctgg tcgctcccgg tgcaaacgct ggtgttcatc acctcgttga cgtttattcc
    12001 ggcaatttta ctgatgatga ccagtttcac ccgcatcatc attgtttttg gtttattgcg
    12061 taacgcgttg ggaacaccct ccgcgccacc taaccaggta ttgctgggac tggcgctgtt
    12121 tttgaccttt tttattatgt cgccggtgat cgataaaatt tatgtcgatg cgtaccagcc
    12181 attcagcgaa gagaaaatct caatgcagga ggcgctggaa aaaggggctc agccgctgcg
    12241 tgagtttatg ctgcgtcaga cccgcgaggc ggatttaggg ttgtttgcca gactggcgaa
    12301 tactggcccg ttgcagggac ctgaagctgt gccgatgcgc attttgctcc cggcctacgt
    12361 gaccagcgag ttgaaaaccg catttcagat aggcttcacg attttcattc cttttttgat
    12421 tatcgacctg gtgattgcca gcgtgttgat ggcattgggg atgatgatgg ttcccccagc
    12481 cacaattgct ctgcccttta aactgatgct gtttgtactg gtggatggct ggcaattgct
    12541 ggtcggttcg ctggcgcaga gcttttacag ctagagaggc aaaatgacac ctgaatcggt
    12601 catgatgatg gggactgaag cgatgaaagt cgcgctggca ctggctgccc cactgttgct
    12661 ggtggcgctg gtcacgggtc ttatcatcag tattttgcag gccgccaccc agattaacga
    12721 aatgacgctg tcgtttattc cgaaaatcat cgccgtattt atcgccatta ttattgccgg
    12781 accgtggatg ctcaatctgt tgctggatta cgtccgcacc ttgttcacta acctgccgta
    12841 tatcatcggg taggccgtac aatgatgcag gtaacaagcg atcaatggct ttcctggtta
    12901 agcctgtact tctggccgtt actgcgcgtg ctggcgctca tttctaccgc accaattctg
    12961 agcgaacgca gcgtgccgaa acgggtaaaa ctgggtctgg caatgatgat cacgttcgct
    13021 attgccccat cattacctgc caacgatgtt cctgtttttt cgttctttgc tctgtggctg
    13081 gccgtgcagc agatcctgat cggcattgcg cttggtttta ccatgcaatt tgcctttgcc
    13141 gctgtgcgaa ccgctggtga aattatcggt ctgcaaatgg ggctgtcatt tgcgacgttt
    13201 gtcgatccgg ccagccatct caatatgccc gttttagcgc gtatcatgga tatgctggcg
    13261 ttactgctgt tcctgacatt taacggtcat ttatggttga tttcactgct ggccgatacc
    13321 tttcacaccc tgccaattgg tggcgaaccg ttgaacagca atgcgtttct ggcactcacc
    13381 aaagcaggga gtttgatttt ccttaacggg ctgatgctgg cattaccgct cattactctg
    13441 ctgctgaccc tgaatctggc attaggctta cttaatcgta tggccccgca attatccatt
    13501 tttgttattg gatttccatt aattctgact gtcggtatct ctttaatggc ggcattaatg
    13561 ccgttaattg cgcctttttg cgaacattta ttcagtgaga tgtttaattt gctggctgat
    13621 attattagtg aattgccatt aatataattc cgtaacgttt atcatgttat cctaaggatt
    13681 atccgaaaaa taatacctac gaacatcttc caggatactc ctgcggcgaa atatttgttt
    13741 taagctcact cacatatcgc aacatttact ttactttaag acaattccag gcaaattata
    13801 caacacttta cgggatagta agtccgcctg aaaaatcgcg agagtggcgc attaggtgac
    13861 ccatgttgtt ccgtttagtc atgatgaaat attcaggtaa ggggaattat cgttacgcat
    13921 tgagtgaggg tatgccatgt caacgattat tatggattta tgtagttaca cccgactagg
    13981 tttaaccggg tatctgttga gtagaggggt taaaaaaaga gaaatcaacg acattgaaac
    14041 cgttgatgac cttgccatag cttgtgattc acagcgccct tcagtggtgt ttattaatga
    14101 ggactgtttc atccacgatg cttctaacag tcagcgtatc aagctcatca ttaatcaaca
    14161 tcccaatacg ttatttatcg tttttatggc aattgccaat gttcattttg atgaatatct
    14221 attggtcaga aaaaatttat tgatcagttc taaatcgatt aaaccggaat ctctcgacga
    14281 tatccttggc gatattctga aaaaagagac aacgataacc tcgtttttaa atatgccgac
    14341 gttatcattg agccgaaccg aatcgagtat gttgcgaatg tggatggcag gtcagggaac
    14401 cattcaaatc tctgaccaaa tgaatatcaa agccaagacc gtttcatcgc ataaaggtaa
    14461 tattaaacgt aagatcaaaa cgcataataa acaggttatc taccatgtcg tccgactgac
    14521 ggataatgtg actaatggta tttttgtcaa catgcgctaa cacattctga ctggtggttt
    14581 cccaccagtc aggccgaatt agattactct gctttctcca caaagatacc gtcctgatgc
    14641 cctgcttcat taaagaacca gatgccgagc gggtagtctt ccagcgaaac caggtacatt
    14701 gtgccttcac taaactcctc aactgccagt accacgccag gacgacgcgg accgccatcc
    14761 gttttgactg ttacccgatc attcaccttc atcgttttcc tcctgtggct ttgtgccagt
    14821 gtagaacaat ttcgtttttc tggcagcgcc aggcgcgcgc gagtgctgat tttctcgacg
    14881 gtctatactt aagagatgcc aggcggactt aacgactggc cgcaacaaca gagtaacggt
    14941 tgcgaggaaa gatgatgaaa accgcaaaag agtacagcga taccgcaaaa cgtgaggtca
    15001 gcgtcgatgt cgatgccctg ctggcggcga tcaatgaaat tagcgaaagc gaagttcatc
    15061 gcagccagaa cgattctgaa cacgttagcg tcgatggacg tgaatatcat acatggcgtg
    15121 aattggcgga tgccttcgaa ctggatattc atgacttcag cgtctctgaa gtgaatcgtt
    15181 gaatgcacaa taaaaaaatc ccgaccctga gggggtcggg atgaaacttg cttaagcaag
    15241 aagcacttaa aaaattcgtt acaccaggaa atctgatgtg ttcatcacct tatccgcaat
    15301 ttttttcgct gacaagaaaa tattcgtgat atgaatgatt aacgctactt atgaatagaa
    15361 atgtgacgcg cgtcacccct tggtggctct ggcgtcaacg ggatcgctcc cgaaaaaatc
    15421 atctttgata atttgtgctg ttttgagaat catcctggta aggggagtac tgcgcaacca
    15481 tgctttcaat tcaacaacca ctactggttt ttagcgatct tgatggcacc ctgctggaca
    15541 gtcatagtta tgactggcaa ccagctgccc cctggctcag ccgtttacgc gaagcgaatg
    15601 ttcccgtcat tctctgtagc agtaagacat cagccgaaat gctgtacttg caaaaaacgt
    15661 tggggctaca aggtttaccg ctgattgccg aaaatggcgc agtgatccag cttgctgagc
    15721 aatggcagga tatagacggt tttccacgca tcatctcagg tattagccat ggcgaaatca
    15781 gccaggtttt aaatacgcta cgcgagaaag aacattttaa attcacaact tttgatgatg
    15841 tcgacgatgc aaccatcgcc gaatggacgg gattaagccg tagccaggcg gcgctgacgc
    15901 agctacatga ggcgtcggta acgctaatct ggcgcgacag tgacgagcgt atggcacaat
    15961 ttaccgctcg tctgaacgaa ctgggcttgc agtttatgca gggtgcgcgc ttctggcacg
    16021 tcctggatgc ctctgccgga aaagatcagg ctgccaactg gattatcgcg acctatcaac
    16081 aatcgtcagg caaacgccca accacacttg gcctgggcga tgggccaaac gatgcgccct
    16141 tactggaggt aatggattac gcggtgattg tgaaagggtt aaaccgtgaa ggggtgcatc
    16201 tgcatgatga ggatccggcc cgcgtctggc gaacgcagcg tgaaggaccg gaaggctggc
    16261 gtgaagggct ggaccatttt ttctccgccc attaagcgtt atcgctcgcg aatacccgat
    16321 tgcgcccggc ctgtttagcg agataaagtc gacggtcggc cagtgactgg agttgttcaa
    16381 aatcataatc accggtttcc tcgctgctgc ttacccccag cgaggcactg atgcgtatcg
    16441 tcgtactctt ggcgatcaac atctcttttt catttagctt caggcgaata cgttctgcaa
    16501 cctccgcagc ctgcgtcagg ctcgcgcctg gcagaatcac acaaaactcc tcaccaccga
    16561 cccgcccggc aacatcctgc gctcgcaagg aactgctaat taatccggca gcatgagaaa
    16621 gaacacggtc gcccgcctga tgaccaaagc ggtcattaat cgctttaaaa tggtcaaggt
    16681 cgacctgaat gacagaaaaa ggatgttggt gcgtctgaca caatttagcg agcggacggg
    16741 ctttttcgaa cagtgcgcca cggttatata aacgcgttaa ggtgtcgtgc cacgcctgcc
    16801 agtgcaacga gctttgcaaa acatacatgt tgctgaccat ccggcgaatc acataccagg
    16861 agatgagtaa catggtggta aagagcgccc acagcagggt taatgcaatg ctgatactgc
    16921 cgaaatcgcc gcgcacgcct tcgcttagcg tatggacgcg caccagcaca ccgtcgaaat
    16981 ggtccagacg ttcccagcta acatagcgac tgttcatacg aatgccgccc cgcgtgtcat
    17041 gttccattgc ctgcgccagc aaggccagtt cacgaggatc aaaaatattc cctgttggat
    17101 ggtcaggatt ggaagaggtc aataatctca gcttactgtc atagagctga tactcaccat
    17161 cgaggttttt atcgatggcg tttcttaaaa attgctgcat ggtacgcacg ggaatactca
    17221 tccccagcac gccataccag tagttttcac tgtctaccgg aacactgacg gtaacctgcg
    17281 gttcagtatt gctggcgtgt tccggttgcg aagtgaacca gcgtaccgcg cggtgacgat
    17341 tttctcgttg cgaatggccg acaaaccagg gttgggtgac atagccgtaa taacgcgttg
    17401 gtacattgcg cgtaaacaag gtcggctgcg tcgaaacgta aaatccggca cgcgagacat
    17461 acatcgcctg ttcaaccatc gacgaggagt tgtgtgccag tcgcagcaag taaccaactt
    17521 ccagtgcagc ggtaatttca ttgtcgaggc tttcattttc gcgagacagg agattcccct
    17581 cgctgactaa tgcatccgac acaccattga ctggcagggt gcgtcgtcgg ttgagttcga
    17641 tttgccaggc gtgctcgtcg cgatgctgtt cgaactcggt tacggcatta cgcagagagg
    17701 tgaaatccag tggcgctacg agagcttcgc gcatgccatt acgcaaaaag atcagtttgt
    17761 cgacgttata ctgcaaatgc ttatcgagcg cgttggctac gttttccaga tgattacgct
    17821 ggctggagat ataggcatct tccagcacca ccacttcacg ccaggtgagc aaggtggaaa
    17881 aaagcaatac cacgataaag cagagattaa cgacatgacc aggccccagg cggcgtgcga
    17941 gttttttcaa ccagctctgg ttttccattt ttgtctcgtg ctgcaccctg acgactccca
    18001 acctgctttt ttatgattct ggcataacct ggcgatagcg taccgcaaaa cagcgataat
    18061 tttataatgg tggcatgata agacgcgaca gacatcgata gttttataac gtaggcatga
    18121 taagacgcga tagcgtcgca tcaggcaatt cagtgcagac actggcacac ccgaccggat
    18181 gcgacgtgag cgccacatcc ggtcgggtgt taatgatacc ctttcaaact tcttccgcag
    18241 aacgactccc ctcccccgga acaagctcgg tttcatgaaa agcttcccgc ttgaccccgt
    18301 aaccgtcata ccaacgacac tcaaccatac cgctggagta tccggtgaca atcatccgcg
    18361 ggccgccctc tttaaccgta acttcctcac taaccataaa gctcatactc gcctcctttt
    18421 ttcgagtgaa acttgttcac cttagttgaa gatggcgaat tttgcataat agcctgtgcg
    18481 agtattaatg tgcctgaccg cgtattttcg ctacggcttt cactcccagc accacgatgc
    18541 cgccgatgat aaatccaaga atcagattta aaacagtcgg taatatcatc gccaccactg
    18601 cactttgctg cccggcgaaa tgttcaatgg cgtgatgcag cggcgcaatt ccatgtacca
    18661 caatcccacc gccgacgagg aacatcgcca gcgtgccgac aatcgataac gccttcatca
    18721 accagggcgc aatgatcaat aaccctttgc ctaatgcctg catcagcgcg ctggattttt
    18781 ccgccagcca ataccccagg tcatcaatct taacgataac ccctaccaga ccgtagacgc
    18841 ccacggtcac taccagcgcg atgcctgaaa gcaccagcac ctgattaagc aacggcgctt
    18901 cggccacaat ccccagcgtg atggcgacga tttccgcaga caatataaaa tcggtacgaa
    18961 tcgccccttt tattttgtct ttttcaaact tcagcggatc ctgcgccgcc agcttctcca
    19021 gacgctgctg actctgcgcc ggatcttctt tatgtttacg cgcctccagc atatgcagca
    19081 ctttctctac cccttcaaag cagagaaacg cgccgccaat catcaacagt ggcgtaatcg
    19141 cccacgggat aaacgcactg atgatcagcg ccagcggcac cagaatcact ttattgatca
    19201 gcgatccttt cgccacgccc cagaccacgg gaagttcccg gttggcgcgc acgcctgcaa
    19261 cttgttgcgc attgagcgat aagtcatccc ctaatacacc ggcggttttc ttcgccgcca
    19321 gtttgcccat cacggagata tcgtccagca gtgtggcgat atcatcgagc aacgtcagta
    19381 aactactccc ggccaaaagc actccctctc atttttattg ttgaagggtg taagtatgaa
    19441 gcaaaaacgg cagctctgat acttgcttca caggtcaaca tttatttaac aatcaagtgc
    19501 aattatatct ctcgccagca gaatgatttt cacgtttact atgaaccacc tttttatttc
    19561 agccgtgagg gattatgcgt ttccgccagt tgttaccgct ttttggcgcg ctgtttgcgt
    19621 tgtatatcat ttggggctca acctattttg tcattcggat tggcgtggaa agctggcctc
    19681 cgttaatgat ggcgggcgtt cgattcctgg cagccggtat tttattgctg gcatttttgc
    19741 tactgcgcgg acacaaactt cccccgctac gtccgctgct caatgccgcg ctgattggcc
    19801 tgttattgct ggctgtcggt aatggcatgg tgacggttgc cgaacatcaa aatgttcctt
    19861 ccggcatcgc cgccgttgtg gttgcaaccg tgcccctctt taccctgtgc ttcagccgcc
    19921 tgtttggcat taaaacgcgc aaactggaat gggtgggtat cgccattggg cttgccggaa
    19981 tcatcatgct caatagcggt ggaaatttaa gcggcaatcc gtggggcgcg attctgattt
    20041 taatcggctc gattagctgg gcgtttggct cggtttatgg ctcgcgcatt accttacctg
    20101 tagggatgat ggcgggtgcg attgagatgc tggcggctgg cgtggtgtta atgattgcgt
    20161 cgatgattgc gggtgaaaaa ctgacggcgc tcccttccct ttcaggcttc cttgcggtcg
    20221 gctatctggc gctgtttggt tcgattatcg ccattaacgc ttatatgtat ttaatccgca
    20281 acgtcagtcc ggctctcgcc accagctacg cctacgttaa cccagtggtc gcggtcttgc
    20341 tgggtacggg gctgggtggc gaaacgctgt cgaagattga atggctggcg ctcggcgtaa
    20401 ttgttttcgc ggtggtactg gtcacgttgg gaaaatatct cttcccggcc aaacccgttg
    20461 tcgcgccagt tattccggat gcatcaagcg agtaaatgaa tcccctgcgt gtcgatctgc
    20521 gcgctggcac cttcgccgca gatccactct tccagacgct cggtaagcgc cgcatccgtc
    20581 agcttctcgc gcccgcgtaa cgcacattcc cagacaatca atacgcgcca accgagttcc
    20641 tgcaaatggc tgatatcacg acgatcgcgc tcgacatttt tacctatctt ctccagccaa
    20701 aaatctgttc gcgtggcggg gactttaaac aggtagcaat ggtgatggtg ccagaagcaa
    20761 ccgtgggcaa atatcacaca gcgaaattca tcaaggacaa aatccgggcg tccgggcaga
    20821 ctggcgtcct gaacgcgaaa cgccaggccc tgcccagtta acaaactggc gaggcgcttc
    20881 tcaatcgccg tatcacgcgt ggcaatcgcg cgcatatttt tgctgcgggt ggccttatcg
    20941 tgaacgtcga ccatgttgtg cctcttgctg acgcaacgcc accgcctgtt tgatttttgg
    21001 ctcaagcagt tttgccaccg cggcaaagac cggcacgacc accgagttac cgaactggcg
    21061 ataagcctga gtgtccgaaa ccggaatgcg gaatttcgct tctcccggcg cttcaaaacc
    21121 cattaagcgc gcacattccc gaggcgttaa ccgacgtggg cgatgttgct gattcagcgg
    21181 atcgtcaaag tctttctcac ccgttgccat atcccagccg cgatcgatta aaatttccgc
    21241 gccatctttg taataacgcg cagacagcgt acgcgtgacg ctttgcggat tgttcggata
    21301 aaccattcca taaccgaagc cgttaccgcg cgcctgatgt tttttcgcat atcgatagag
    21361 gtacttccac agtaccggcg tcaggatata tttcgcctcg accatcgggt ccagcagctg
    21421 cgccagcgtc actcgctgtg cagggaaaca tttgctgata tcacgcagag taaaatcggc
    21481 tttcagattc agatcgcgac gaaaacccac cagcacgatg cgttcacggt gctgcggcag
    21541 aaaatgtttg ccgtcgatga ttttcggatc gtccggcccg ttatcttctg catcagccac
    21601 gtcatagccc agttcgtcca gcgtctgcat gatgatgcgg aacgttttac cctggtcgtg
    21661 acttttcagg tttttgacgt tttcgagcac aaacatcgcc ggacgacgcg cgtcgataat
    21721 gcgcaccaca tcaaagaaca gcgtgccctg ggtatcgcag gcaaaaccgt gcgcccgccc
    21781 gagcgagttc tttttcgata cgccagccag cgaaaatggc tgacaaggga aaccggccag
    21841 taaaacatcg tgttcaggaa tgtgttgacg aatatgttcc gccgccgcct catcactcac
    21901 gccttcttga tggctgaggg tgatgtcgcg gatatcctca ttaaaatgat gcgtcgccgg
    21961 gtcgcaataa tggttggctt tataagtgcg taccgcatgt ttgttccatt cgctggtaaa
    22021 cacgcactgt ccaccaatcg attcaaaacc gcgacggatg ccgccaattc cggcaaatag
    22081 atcgataaag cgaaacgcat aatgcggatg atgtgccggt ggtttgggta acaacgtttg
    22141 cagatgggcg aactctttct cacttaaacg gtgccatgcc gagtcattcg ccagcgcacg
    22201 ttttaaaatt gccgcgctcc agtgattctc acccacacca ttaagctgcg ccaccagcgt
    22261 tttaacatca taaatttgca gcagtttctc cagcattgcc tgtgcggcat tcccggtgct
    22321 gtatgaatcg gttactgata tattttcctg catagattta accggccatc tgagatggcc
    22381 gacaggttaa cataattact gccgtttagg gccacgcgct cagccagcag gtgaaaacgc
    22441 atctatcacc ttgtgatcga taccttcatt ctcccccgcc agttcggcac cgagcttcgc
    22501 cataaactcg actaaaaatt gcgcgttgtg ctgtgccagc tgcttgcctc ttgcggtttg
    22561 catggtttgc ggtaatttca gtaactttgt ctggaaatga tccagcgcgt agcgtttatc
    22621 atcaagcggg cgatgctgtg cgaacggatc ttcgccatca aacagcgcca agcccaatgc
    22681 ccctgaaacg gcaaatacgc gcgccaggcc aatcgccccc agggcttcca gccgatccgc
    22741 atcctggacg attttagctt ccgttgttaa gggggcgatt tgcgcgctga aactgtgagc
    22801 ggcaatggca tgacaaacgg cctcaatttt ctccgccgga aattgcacga actctt
//
LOCUS       DAZGLJ010000054        22452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000054 DAZGLJ010000000
VERSION     DAZGLJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.054"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            81..329
                     /locus_tag="ACHK49_001921"
     CDS             81..329
                     /locus_tag="ACHK49_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO9262577.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            307..1437
                     /locus_tag="ACHK49_001922"
     CDS             307..1437
                     /locus_tag="ACHK49_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO9262578.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHK49_001923"
     CDS             complement(1483..2121)
                     /gene="ompW"
                     /locus_tag="ACHK49_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO9262579.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2361..2450
                     /locus_tag="ACHK49_001924"
                     /pseudo
     CDS             2361..2450
                     /locus_tag="ACHK49_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2478..3221
                     /gene="yciC"
                     /locus_tag="ACHK49_001925"
     CDS             2478..3221
                     /gene="yciC"
                     /locus_tag="ACHK49_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO9262580.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            3251..3790
                     /gene="yciB"
                     /locus_tag="ACHK49_001926"
     CDS             3251..3790
                     /gene="yciB"
                     /locus_tag="ACHK49_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO9262581.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3895..4293
                     /gene="yciA"
                     /locus_tag="ACHK49_001927"
     CDS             3895..4293
                     /gene="yciA"
                     /locus_tag="ACHK49_001927"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO9262582.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHK49_001928"
     CDS             complement(4333..5052)
                     /gene="tonB"
                     /locus_tag="ACHK49_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO9262583.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            5276..5572
                     /gene="yciI"
                     /locus_tag="ACHK49_001929"
     CDS             5276..5572
                     /gene="yciI"
                     /locus_tag="ACHK49_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO9262584.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5871..7124
                     /gene="kch"
                     /locus_tag="ACHK49_001930"
     CDS             5871..7124
                     /gene="kch"
                     /locus_tag="ACHK49_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO9262585.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(7179..7352)
                     /gene="yciY"
                     /locus_tag="ACHK49_001931"
     CDS             complement(7179..7352)
                     /gene="yciY"
                     /locus_tag="ACHK49_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO9262586.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            7495..8955
                     /gene="cls"
                     /locus_tag="ACHK49_001932"
     CDS             7495..8955
                     /gene="cls"
                     /locus_tag="ACHK49_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO9262587.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8990..9319
                     /gene="yciU"
                     /locus_tag="ACHK49_001933"
     CDS             8990..9319
                     /gene="yciU"
                     /locus_tag="ACHK49_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO9262588.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(9372..10376)
                     /gene="oppF"
                     /locus_tag="ACHK49_001934"
     CDS             complement(9372..10376)
                     /gene="oppF"
                     /locus_tag="ACHK49_001934"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO9262589.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10373..11386)
                     /gene="oppD"
                     /locus_tag="ACHK49_001935"
     CDS             complement(10373..11386)
                     /gene="oppD"
                     /locus_tag="ACHK49_001935"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO9262590.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(11398..12306)
                     /gene="oppC"
                     /locus_tag="ACHK49_001936"
     CDS             complement(11398..12306)
                     /gene="oppC"
                     /locus_tag="ACHK49_001936"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO9262591.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(12321..13241)
                     /gene="oppB"
                     /locus_tag="ACHK49_001937"
     CDS             complement(12321..13241)
                     /gene="oppB"
                     /locus_tag="ACHK49_001937"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO9262592.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEEKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(13327..14958)
                     /gene="oppA"
                     /locus_tag="ACHK49_001938"
     CDS             complement(13327..14958)
                     /gene="oppA"
                     /locus_tag="ACHK49_001938"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO9262593.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            15242..15538
                     /locus_tag="ACHK49_001939"
     CDS             15242..15538
                     /locus_tag="ACHK49_001939"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9262594.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(15696..16343)
                     /gene="ychE"
                     /locus_tag="ACHK49_001940"
     CDS             complement(15696..16343)
                     /gene="ychE"
                     /locus_tag="ACHK49_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO9262595.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(16374..16427)
                     /gene="ychE"
                     /locus_tag="ACHK49_001941"
     CDS             complement(16374..16427)
                     /gene="ychE"
                     /locus_tag="ACHK49_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO9262596.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            16820..19495
                     /gene="adhE"
                     /locus_tag="ACHK49_001942"
                     /gene_synonym="adhC"
     CDS             16820..19495
                     /gene="adhE"
                     /locus_tag="ACHK49_001942"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO9262597.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            19619..20515
                     /locus_tag="ACHK49_001943"
                     /pseudo
     CDS             19619..20515
                     /locus_tag="ACHK49_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(20797..21414)
                     /gene="tdk"
                     /locus_tag="ACHK49_001944"
     CDS             complement(20797..21414)
                     /gene="tdk"
                     /locus_tag="ACHK49_001944"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO9262598.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            22019..22432
                     /gene="hns"
                     /locus_tag="ACHK49_001945"
     CDS             22019..22432
                     /gene="hns"
                     /locus_tag="ACHK49_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO9262599.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLASVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
BASE COUNT         5897 a         5475 c         5248 g         5832 t
ORIGIN      
        1 tcaggctgat ttttaatgtt caaccccggt cgttgcccac cagctatagt ggcggcgacc
       61 atgattagcg aacgacgctc atggcaagac ttattctgct cactgagtgg gcaaaagagg
      121 aattcagtga accggtccca actccgagta cgttaagtaa atacgctaaa gccggaatga
      181 tatttcctct ccccaaaaaa gttggaagac gctggcgagt ggatccgcaa gctcgctttg
      241 tcggaatggt aaacaagccg gaggtgatcg ccacagatca ccctgctttg aagaggatac
      301 tggaagatgg cgcgcccgcg aaaatataaa accgatgttc cgggattatc tccgtatttt
      361 gacaaaagaa ataacaaagt ttactggcgt tacaggcatc ccataacagg caaaaatcac
      421 ggtctcggca gtattgacca gaaactggca gaaactattg cagcagaagc gaacagccgt
      481 cttgcccggc agcaaatgga acaaatgctc agtctgcagg agaaaattat tagtgatacc
      541 ggcggttcat caaccgttac catttttctg aataattaca gaaaaattca acaggaaaga
      601 tatgaaaacg gcgagatcaa actcaacacg ctgaaacaga aagcggcccc tctcagggta
      661 tttgatgaac gttttggcac cagaccgtta gatgccataa ccgtaaaaga tgtggtatca
      721 gtactggaag agtacaaggc cagaggacat aacagaatgg gacaaatttt caggaaagta
      781 ctgatcgatg ttttccggga agctcagcaa acgggcgatg tcccgccagg ctttaaccct
      841 gcagaatcgg caaaaaaacc gcaggtgcgg atatcaagac agcgactgac ttttgatgag
      901 tggatgatga tttataacgc agcggaaaag gatggttact ttttacagcg cggtatgctg
      961 ctggcactga tgacaggcca gcgcctttca gatatttgca aaatgcaatt ttcggatatc
     1021 cgggatggtt atcttcatgt cgaacagcaa aaaacaggaa cccggattgc catccctctg
     1081 gctctgcgtt gcgataaatt aaatctcacc ctggatgatg tggtgtcatc ctgccgcgat
     1141 tgcgttctta gtccgtggct attgcaccac catcacgcga aagggacagc taagcgcggc
     1201 gggatggtta agccagcaac attaaccgtt gcatttaaaa aagcccggga ttctgtggat
     1261 tacaactggc gtgctaatgg cacccctccc tctttccatg agcagagatc tttatcagag
     1321 cgattgttca gagagcaggg ggttgatacc aaaattttgc taggccattc gaatcaaaaa
     1381 atgaccgata tttacaacga cgcacgcggt aaggaatgga aaaaactggt catttgatga
     1441 ccagttttgc agaggggttt tgcagaggtt ttgcagagaa aattaaaaac gatatcctgc
     1501 ggagaacata aacacccacg gatccagtcg taccgagtct tttacggtag taacaccaga
     1561 tttgtactta gccgtggtat cgatatccat gtaccacact gacatgttaa ccaaccagtc
     1621 acggttaatc agataatcaa cccccacctg cccggcagct ccccaggaat ctttcagact
     1681 gagatcggaa agccccgcct ctttgccatg atcgttaaat ccattatcaa agaaggtggt
     1741 gtagttaata ccggccccaa cgtaaggacg gaatttgctg ctggcatcac caaaatacca
     1801 ctgcgccatc agtgttggcg gcagatggtg aacggttgca atatcgccgg tcgcccgggt
     1861 gccgatttta tggcggaacg gcgtcgctgc cagtaattcc acaccaatgt tgtcggtcgc
     1921 catataagta aacgtcaagc ccagttgagt gttattggtc acgctgaatc cacccagact
     1981 tcctaacgta ccaccagcac cttctgttgg acgtacggtt gcagaacctg cacgcataaa
     2041 aaattcgcct gcttcatgcg caaaggcact gccagaaaga agagttgtta ctgccaaagc
     2101 cgccactgtt aactttttca tatccgctcc gtcgttatgg ttataaaaac gtgacgaata
     2161 tacctacatt ggagtaacaa atgatcctac atagatcaca ttacatatga taattcacca
     2221 tttattgatc tggattaatt tagaagttgc attgagaatc ctggttaatg tgatttaaat
     2281 caatttttaa atattttctc tacgcaaata ttaactcgat cgccacttgc cgcggtttgc
     2341 ccacgcctga aggccagcaa ttacctccgc tttacaaaga tatgcttttc catacaagac
     2401 ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc cgctaattaa cacctgcata
     2461 aaactcaagg agagtgcatg tctatcacgg cgcagtccgt ataccgtgac accggaaatt
     2521 tcttccgtaa tcaatttatg accattctgt tggtatcgtt gctatgtgcg tttatcacag
     2581 tggtgttagg gcatgttttc tcacccagtg atgcacagct tgcgcagctc aatgacggcg
     2641 tgcccgttag cggcagtagt gggttgttcg acctggttca gaatatgtca ccggaacagc
     2701 aacaaatttt gctgcaggct tcagcggcgt ccactttttc aggattaatc ggtaacgcca
     2761 ttctcgccgg aggcgtaata ttaattatcc agctggtgtc tgcgggtcag agagtcagtg
     2821 cgctgcgagc tattggtgcc agtgcgccga tattgcccaa gttatttatt ctgatttttc
     2881 tgactaccct tttagtacag attggcatca tgctggtggt cgttccggga attatcatgg
     2941 ccattttact ggccctggca ccggtgatgc tggtacagga caaaatgggc atttttgcct
     3001 cgatgcgtag cagtatgcgg ctgacttggg cgaatatgcg tctggtggca cccgcagtac
     3061 tgagctggtt gctggcaaaa acactgttgc tgctttttgc ctcttctttt gccgcattaa
     3121 ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa cctgatttct gccatattgc
     3181 tcatctatct gttccgcctg tatatgttga ttcgccaata accttaagca tttgattacg
     3241 gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg gttgtctttt tcgcgtttta
     3301 caagatttat gacatctatg cggctactgc ggcgctgatc gtcgccacgg cgattgtgct
     3361 tatctatagc tgggttcgct ttcgtaaggt tgagaagatg gccctgatca cttttgttct
     3421 ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat gatgagttta ttaaatggaa
     3481 ggttacagtc atttatgctc tgtttgcggg agccctgtta gtcagccaat gggtgatgaa
     3541 aaagccgcta attcagcgga tgctgggtaa agaactcacg ctgccgcaac cggtatggtc
     3601 gaagctaaat ctggcctggg ctgttttctt tatcctttgc ggtctggcaa acatctacat
     3661 cgcattctgg ctgccgcaaa atatttgggt caactttaaa gtctttggcc tgaccgccct
     3721 taccttaatc ttcacattgt taagcggtat ctatatctac cgccacatgc cgcaggaaga
     3781 taaatcctaa ccagactgcc agtccttcgg ggctggcgct gtatctcctc tgttctcctc
     3841 aggaaaatca tagtagcatc gcgcctgtga ttttcctttt aagtcggttt taccatgtct
     3901 acaacacata acgtccctca gggcgatctt gttttacgta ctttagccat gcccgccgat
     3961 accaatgcca atggtgacat ctttggtggt tggttaatgt cacaaatgga tattggcggc
     4021 gctattctgg cgaaagaaat tgcccacggt cgcgtagtga ctgtgcgggt tgaaggaatg
     4081 actttcttac ggccggttgc ggtcggcgat gtggtgtgct gctatgcacg ctgtgtccag
     4141 aaagggacga catcggtcag cattaatatt gaagtgtggg tgaaaaaagt agcgtctgaa
     4201 ccaatcgggc aacgctataa agcgacagaa gcattattta agtatgtcgc ggttgatcct
     4261 gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa aagcctccgg tcggaggctt
     4321 ttgactttct gcttactgaa tttcggtggt gccgttaatt ttaaacagga tattcaccac
     4381 aatcccactg cctggcttac ccggctcata acgccatctg cgcatcgcat ttttcacctc
     4441 acgctcaaac atgttcgcag gcttggctga gaggatttgt acgttatcca cgcgaccatc
     4501 cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg cgcaatgcct gtgctcgtgc
     4561 cggatactgc ggctgattac ggcttaatgc gcgtggtcct gaagccacac tggtaaccgg
     4621 cttgctggtt gcagccgttg ccgtacttga tgtggggcgc gcgggtgccg tattttcaaa
     4681 cggtgatgcc ggacgcgact ctacgggttt gatatcgcgt tttggctgct cctgtacctt
     4741 tttcaccggc ttcggttttg gcttaggttt cggcttcggc ttttcaatga ccaccggtgc
     4801 ttcttttggc ggttcgggga tcggctcagg ttccggttct ggctctacca ccggctccgg
     4861 tggcggctga acggcttgtg gcggttcgag atcagcaggc gcaaccatcg tgacagaaat
     4921 tggctgcgca ggcgcaggta gttcaataac ctgatgtacc gaggtataga gcagacccgc
     4981 cacaacagca ccatgaatgc agaccgaaag taacgtcggc caggggaagc ggcgaggtaa
     5041 atcaagggtc attgaagtca taatcatttc agtagaaaaa ccaggtctcg attttaaatg
     5101 caaatagcaa tcatattcaa taaggcaaga cgatcaggat cagtgaaaat gtcatttttg
     5161 gcgtaattga ccttaattta tgcatggtct taacaaagac atcatctttc cgttgcagat
     5221 cttcgccctt tcaaataacg tactttacaa ctttcccgaa caaggagttg tgcccgtgtt
     5281 gtatgttatt tacgctcaag acaaagctga tagcctcgaa aaacgccttt ccgttcgtcc
     5341 ggcacattta gcacgtttac agttactgca tgatgaaggt cgtttgttga cagctggtcc
     5401 aatgccagcg gtagacagta acgatccagg ggctgcggga tttactggct caaccgttat
     5461 tgctgaattt gaatcgctgg aagcagctca ggcctgggcc gatgctgacc cgtacgtagc
     5521 ggcaggagtc tatgaacacg tttcagtgaa accatttaag aaagtgttct gaatagagat
     5581 tcaatattta agggcacggt ttttgcaacc gtgttcttgt ttggcctttc cttatagccg
     5641 gtcagtgcat tcaccaccac aagcagataa taactttatc tgtatttctt tatatgatgt
     5701 gaacatattc aggaaaatgc cctttcctga cgcagagttg ctcgctcaaa acctttgatt
     5761 actgccgata aagtcatcta ctattgttta acacattcac gcgctgcatg tatgttcaga
     5821 aaatgtgcac gctaagataa ttaaacaaaa ttgtggttac agaggtatca gtgagtcact
     5881 gggctacatt caaacaaaca gcaacaaatt tatgggtgac ccttcggcac gacattctcg
     5941 cgctggccgt ctttttaaat ggattgctta tttttaaaac aatctatggt atgtcggtca
     6001 atttgcttga tattttccat atcaaagcat tttcagagtt ggatctctcc ttgctggcaa
     6061 acgccccact atttatgctc ggcgtctttc ttgtcctgaa ctccattggc ttactgttcc
     6121 gggcaaagct cgcatgggca atcagtatca ttttgttgtt gatagcgcta atttacaccc
     6181 tgcattttta tccctggctg aaatttagta ttggattttg catttttacg ctggtgtttt
     6241 tgctgatact gcgcaaagac ttctcccaca gtagcgccgc agccgggaca atttttgcat
     6301 ttattagttt cacgacatta ctgttttact caacctacgg tgcgctttat ttaagcgaag
     6361 gttttaatcc gcgaatagaa agtttgatga ccgcgttcta tttttcgata gaaaccatgt
     6421 caaccgtcgg ctatggcgat attgtccctg tttctgaatc agcacgattg ttcactattt
     6481 cggtcattat ttccggcatt accgtttttg ccacatctat gacctcaatt tttggtccgc
     6541 ttatccgcgg gggattcaac aaacttgtaa aaggaaacaa tcatacaatg catcgtaaag
     6601 atcattttat tgtttgcgga cattcgattc tcgccatcaa tacgatcctg caactgaatc
     6661 aacgcggaca aaacgtaacg gttatcagca acttgcctga agatgatatc aagcaacttg
     6721 agcaacgctt aggcgataac gctgatgtta tccccggtga cagtaatgac agttcagtat
     6781 taaagaaagc gggaatcgat cgttgccggg ccattctggc gctgagtgat aacgatgcag
     6841 ataacgcgtt tgttgtactc tcggcaaaag atatgagcag tgatgtcaaa acagttctcg
     6901 ccgtcagtga tagcaaaaac ctgaataaga ttaagatggt acatccggat atcattctct
     6961 caccgcaact gtttggcagc gaaattctgg cgcgagtttt aaatggtgaa gagattaata
     7021 atgatatgct cgtttcaatg ttgttgaact ccggtcatgg cattttcagc gataacgatg
     7081 aacaagaaac gaaagctgac agtaaagaat cagtgcaaaa atagagttta aagacgaaag
     7141 agtaaactta ttgggcaccg caatggtgcc cagtatattt agatattgta gatcgcaaac
     7201 aataacgagt tacgctgttt gtttagaatg cacttcctga tgctgtggat acgcatattt
     7261 cggcgcgatt gcccctcaag ccaacgcgat ttacgtcggc tagcctgacg ctgcatgcgc
     7321 cagcgcccca cttccgttct actccgcttc atgttaacta ctctatgcaa taacagaatg
     7381 gtcattatac ccccaccccc gacgctgacc agtgcttttc ccgcgttttt atttgccaga
     7441 tgaatccata tgcgtaaact cataacaatg cgctttcaaa aggatttcta atctatgaca
     7501 accgtttata cgttggtgag ttggttggcc attctgggat actggttgct cattgcaggc
     7561 gtaactttac gcattctaat gaaacgacgc gcagttccct ccgcgatggc ctggctgttg
     7621 attatttaca ttctgccgtt agtcggaatt attgcctatc ttgccgttgg cgagctccat
     7681 ttaggcaaac gccgcgctga gcgcgccaga gcgatgtggc cttccaccgc aaaatggctt
     7741 aacgacctta aagcctgtaa gcatatcttc gccgaagaaa atagcagtgt cgctgcgcca
     7801 ttattcaagc tttgcgagcg tcgtcagggg atcgctgggg tcaaagggaa tcagctacaa
     7861 ctgatgaccg agtcagatga tgtgatgcag gcgttaatcc gcgacatcca gctcgcgcgc
     7921 cataatattg agatggtgtt ttatatctgg cagcccggcg gcatggcgga ccaggtggct
     7981 gaatcattaa tggcggctgc acggcgtggt attcattgcc gattgatgct cgactccgcc
     8041 gggagtgtgg cttttttccg cagcccgtgg cccgagctaa tgcgtaatgc cggtattgaa
     8101 gtggtcgaag ccttaaaggt caatctgatg cgtgtgtttt tacgccgtat ggacctgcgc
     8161 caacatcgca agatgatcat gatcgataat tacatcgcgt acaccggcag catgaatatg
     8221 gtcgatcctc gctacttcaa acaagatgcg ggcgtagggc aatggattga tctgatggcg
     8281 cgtatggaag gccccatcgc caccgcgatg gggattattt attcctgcga ctgggagatt
     8341 gaaaccggaa aacgtattct gccgccacca ccagatgtca atattatgcc gtttgaacag
     8401 gccagcggtc acaccattca cacaattgct tctggccccg gctttccgga agatctcatt
     8461 caccaggcat tattgactgc ggcttattcg gcgcgtgaat atttgatcat gaccacgccc
     8521 tactttgtgc caagcgatga tttacttcat gcgatttgca cggcggcgca gcgcggggtg
     8581 gatgtcagta ttatccttcc gcgaaaaaat gactcgatgc tggtcggctg ggccagtcgc
     8641 gcattcttta cggaactgct tgctgctggg gttaaaattt atcagtttga aggcgggtta
     8701 ctgcatacca agagcgtgct ggtcgatggc gaactaagtc tggttggcac cgttaacctt
     8761 gatatgcgga gtctgtggct gaatttcgag attaccctgg caatcgacga taaaggtttt
     8821 ggtgcagacc ttgccgccgt tcaggacgat tatatttcgc gttcacgtct gctcgatgcc
     8881 cgtttatggc taaaacgtcc attatggcaa cgtgtcgccg agcgactgtt ttacttcttc
     8941 agtccgttgc tgtaaaacgt cgccaacaga ggttaaacag gtagtgacta tggatatgga
     9001 tctaaacaat cgcctgactg aagatgaaac gcttgagcag gcctacgata tttttctcga
     9061 actggctgcc gacaatctcg atccagctga tgttctgctg ttcaatcttc agtttgaaga
     9121 gcgcggcggc gcggagttat tcgatccggc ggaagactgg caggaacatg ttgatttcga
     9181 tttgaaccca gacttttttg ccgaagtggt gattggtctg gcggacagtg aagatggtga
     9241 aatcaacgat gttttcgccc gcattctgtt atgccgcgaa aaagatcaca aactttgcca
     9301 tatcatctgg cgggaataaa taatcgcttt tagcgtgaaa aagggctgac aactgtcagc
     9361 ccttattgtg tttaaagcgg atcgactttc aggcaagaaa cggcgtgtct gaaactcccc
     9421 tccagaacag gacgtgtttt ggcgcactcc ggaccggcaa tcgggcaacg ggtacggaaa
     9481 acacaaccgg aaggcgggtt gatcggcgac ggtaattccc cttccagtaa ctggatggtt
     9541 ttgttcttct ccagatccgg atcaggtatg gggactgccg acatcaatgc cctggtgtaa
     9601 ggatgtagtg gattgtggta gacctcatca taggtcccca gttctaccgc atggccgaga
     9661 tacatcacca acacacgatc ggaaatgtgt tttaccacgg ccaggtcatg agcgataaaa
     9721 attaatgaca atcccatctc acgttgcagc tgctggagca ggttgaccac ctgcgcctga
     9781 attgacacgt ccagcgccga aaccggctca tcgcagataa tcagcttcgg ttcaagaata
     9841 agagcacgag caatcccgat acgctggcac tgcccaccgg agaactcatg cggatagcgg
     9901 ttaatcaggt taggcaataa cccgactttc agcatcatcg ccttcacgcg ctcgcgaact
     9961 tcctggcgtg acattttcgg atgataagta cgcagtggtt cagcgatgat ctcgccgatg
    10021 gtcatacgcg ggtttagcga tgccaacgga tcctggaaaa tcatctgaat atcactgcga
    10081 acggcacgcc attcatcggg cttcatgccc agcaactctt tacctaacca ggcaacatga
    10141 ccgtcggtcg ccttgaccaa accgatgatg gcgcgagcaa aggtggactt accgcatccc
    10201 gattccccta ccacacctaa tgtttcccct tcatacaggc gaagagtgac accatcgacg
    10261 gctttgagcg ttttcggcgg ttgccagaac cactgtttgc catctttgat ttcaaagtgc
    10321 actttaagat cggcgatttc aaggaggact tttcttcctt cagttacagc attcataaca
    10381 gttcctccac cggtttaaag caagcacgca gacggccagg cgtaaactct tccagcggcg
    10441 gtgcgctact acaaatttcc atcgcatgtg gacaacgtgg ctggaacggg caacctttcg
    10501 gtaatcgcag caagtttggc ggattacccg ggatggtcaa cattgtttca ccttccgcat
    10561 caagacgcgg caccgcgttg agcaaaccga tagaataagg atgaacgggt tgataaaaga
    10621 catcgcgcgc attgccatat tccatcgtgc gcccggcgta cattaccagc actttgtcac
    10681 agatccccgc caccacgcca agatcgtggg tgatcataat gatggcggta ttaaattccc
    10741 gcttcagttc atttaatagc gtcatgatct gcgcctgtac ggtgacgtcc agcgcagtag
    10801 tgggttcatc cgcaatcagt agcttaggtc gacatagcaa tgccatcgca atcatgactc
    10861 gctgacgcat gccgccagaa aattcgtgcg ggtacatttt catgcgttta cgcgcttccg
    10921 gcatttttac cgcatcgagc atccgcaccg actcttcaaa cgcctcagct ttgctcatgt
    10981 tcttatgcag catcagcact tccattaact gctcaccgac gcgcatatag ggattcaacg
    11041 aagtcattgg gtcctgaaaa atcattgaga tttgttcagc gcgcagttta ttgagttcat
    11101 gctctggcaa attgaggatt tcacgcccat tgaaggttgc cgatccgcca atacgtccgt
    11161 tggcagccaa caggcccatc aacgcaaatg cagtttgcga tttacccgaa ccagactcac
    11221 ctacaatacc cagcgtttct ccggcacgta gggaaaaatt caaatcattg accgccgtga
    11281 cgtcgccgtc cggggtacta aaggtgacac gcaaatcttt cacgttcagc agtgcgtcag
    11341 cctgttgttg tgcgagcggc acagttgcag tttcaattac gctcatggct gcactcctta
    11401 acgatctttc gggtcgaggg catcacgcaa gccatcgccg ataaagttga aacaaaacag
    11461 cgtcaccacg aggaatcccg ctgggaacaa caataaccat ggagagactt ccatcgagtt
    11521 cgcgccatca ctcagcaatg caccccagct gcttaacggc tcttgcgtac ccaaccccag
    11581 gaagctaagg aaagattcaa agaggatcat gctgggcacc agaagtgatg cgtagaccac
    11641 caccacaccg agtacattcg gcacaatgtg gcgaataaca atgcccgatg tcgatacacc
    11701 gccaacttgt gccgcctcaa taaactcttt gcgcttcaga ctcagggttt gcccacgcac
    11761 aatacgagcc atatccagcc aggaaaccat gcctatcgcc acgaaaatca gcaggatgtt
    11821 ttgaccgaaa aaggtcacca gcaaaatgac gaagaacatg aatgggaagg agttgaggat
    11881 ttccagcaga cgcatcatta ccgaatccac tttaccgccc agataaccgg aaagcgaacc
    11941 ataaagtgtc ccgacgacca cagccaccag tgccgcagca acaccgacca tgagtgagat
    12001 acgcccgcca atcgcaacgc gcacaagcag gtcgcgaccg gatgagtcag taccaaagta
    12061 gtgaccggac tccatatccg gggcgctgga catcatcgcc cagtcagtat cgtcataggc
    12121 aaactgggaa agcatcggtg ccaggattac aaataacgcg atcagcacca gcactatcag
    12181 actggcaacc gccgcacggt tatgcataaa acgtcgacgt gcgtcctgcc acaagctgcg
    12241 cccttcgacc tccagctttt cactgaaatt ttccagcgtc tcgctgtttt tcttacttaa
    12301 catcatcgcg agctccagta tcagtaacgg attttcgggt cgataaccgc atatagcaca
    12361 tcgacaatgg cattaaacaa aatggttaaa gcaccaacca ggatggtcag gcttaacact
    12421 aaggaatagt cacggttcaa tgcgccatta acgaacagtt gtccaatccc cggcaaacca
    12481 taaatggttt cgataaccat agaaccggta ataatgccga caaatgcggg gcccatataa
    12541 gagagcacgg gtaacagagc aggttttaat gcgtgacgta aaatgatccg ccgcataggt
    12601 aaccctttcg cccgggcagt acgaataaaa ttggagtgta atacttcaat catagagcca
    12661 cgggtaatac gcgcaatact ggcgatataa gcgagtgaca acgccaccat cggcaatatc
    12721 atgaatttaa gcgccccacc attccagcca ccgcccggca gccaatgcaa aatgatcgca
    12781 aatatcatga ctaataatgg tgcaaccaca aaactgggga taacaacccc ggtcattgcc
    12841 agccccatca cggtatagtc ccatttggtg ttttgtttta atgcggcaat aacgccagca
    12901 ctaaccccca gtattaccgc aaggaaaaat gctgcggctc ccagtttggc agaaacggga
    12961 aaactggatg caaccaagtc attgaccgaa taatctttat atttaaacga tggaccgaaa
    13021 tcaccgtgcg ccagttgttt caggtagctg aaatactgtg tcatgatcgg atcattaaga
    13081 tgatatttct cttcgatatt ggccatcact tctggcggta aagtacgttc gccggtaaaa
    13141 gggcttcccg gcgcgaggcg catcataaag aacgaaatag taataagaat aaatagcgtc
    13201 ggaatcgctt ccagacagcg acgtagaata aattttaaca ttgccctacc ttctggcctg
    13261 tgccttctgt aatgcgataa aaatcagaca ccgtggagca ggacactcct gccccacgta
    13321 ttgccattag tgcttcacaa tgtacatatt ccgggtatag gtattatcca gcggatcttt
    13381 gccggtatag ccaccaaccc acggtttcac cagacgcgca ttcacgtagt aataaacagg
    13441 aacaatggcc gaatccttat ccagctgttg ttctgcttta gtgtacagag ctgtgcgctg
    13501 cgcctcgtca gtcactttca gcgtttccgc cataatgctg tcaaaggccg ggctcttata
    13561 atgcgcggta ttcatcgagc tgttcgaaag catggtgttc aggaaggaag ttggttcgtt
    13621 gtagtcagca caccagcctg cacgggccac atcaaaagta ccctggtgac gggtgtcgag
    13681 gaacgttttc cactcctggt taaccagttt gacgtttaca ccaatgtttt tcttccacaa
    13741 tgaagaggca gcaatcgcca gctttttatg cagatcggag gtgttataca acaggttgat
    13801 ggtcaacggt ttgtctgcgg tataacccgc ttcagccagc agttttttcg cttcttcgtt
    13861 acgtttttcc tggctccagc caaaccattc cggctgagtc aattttgcgc catcagtata
    13921 cggtggagtg taaccatagg cgggcatgtc gccctgcgct ttcactttat taacaatgat
    13981 atcgcggtcc atacctagtt tcagtgcggt acgcacacgc acatcgttga atggcggttt
    14041 ctggttgtta atttcgtaat agtaagtgca caggtatgga tcaacgtgaa cttcgtccgg
    14101 gatctctttt ttcagcttct ggaacaattc gatcggcatg ttgttataag tcatgtcgat
    14161 ttcaccacta cggtagcggt tgacatcggt aacttcagaa gcaataggca aataggttac
    14221 ctggttaata acggttttcg cgttgttcca gtaggtcggg ctgcgttcaa gaacgattcg
    14281 ttcgtttacg acccaatctt ttaaggtata ggcaccgttg gtgacgatat taccaggctg
    14341 ggtccatttt tcgccgaatt tctcgatagc ggcttttggc accggtgaag ttgatgggtg
    14401 aacaagtaat ttatagaagt acggaacggg ttcacttaag gtgacttcta atgtgtgatc
    14461 atcaatagct ttcacgccga gatcggtaat cggttttttc ccttcaagaa tttcatcaat
    14521 accggcgata tgcccatatt gcagataact ggcatacgga gaagcagtgt tcggatcaac
    14581 agaacgttgc cagctataca caaagtcttg tgctgtgact ggcgtgccat cagaccattt
    14641 cgcatcttta cgcaaatgga aggtccagac tttcgcgtct ttattatccc aggattcagc
    14701 gacgccaggt gctggatgac cgtcaagatc gctgaccagt aagccttcaa acaggtctcg
    14761 gctgatatta gactccggaa caccttcaat tttgtgcgga tctaatgact gaacttctga
    14821 accattgtta cgtaccagtg tttgtttttc cgccagtgtg acgcctgcgg gtacatcagc
    14881 tgccagcgcg acattccctg ccattagcgc agccagaacg ccagctgcta ctaaacttct
    14941 cttggtgatg ttggtcattg ttttttggac tccctcatta taattactgg tattaaccag
    15001 catgtgtaat cccggattgg gaccctgcac aggctcggag actttctgct gtcaggttac
    15061 tttatcgtta cgtattacta tcaccgactt tatttattct ggtgattcag acgatcacct
    15121 tatgtcgatt cgtactgtgt ctcccctgtc gagacacatt attctttcgc gacgctcttt
    15181 aattgttaat tattctcatt accaataaat aatcgtctgg cggttgcccg gaaagtacca
    15241 aatgcggttc ttactcgcca atacattttg caaaattgtt aagcaattct cttttattga
    15301 ccgtatcaga atctatagct tgcatccatg ccttaacaaa catctttcaa ttcaatcaaa
    15361 tagagattgg ctaagcatgt ctatcttttc tgattacagt tctagtagtg aaatgcacaa
    15421 taatttaaca attgattatt atttagcact ttcctctacc aaaggcagtg gaattactaa
    15481 tatcatttca attattcttc agcaagccca ggattatgat gtggcgaaaa taacatgatg
    15541 gtgaggagaa gcagttaagg aaatccttat aactaactgt aatttgagaa gaatattttt
    15601 acctattcct tttgcttttg ataactgcgc gggaacaaag taaaaataat gacgaagtaa
    15661 acggagcatg gcagctccgt ttcatttgaa aggaattaat taagcaggcc ggggaaaatc
    15721 cccttaatac cagtaacgat aaattcaatc cccaatgcca tcagcaatag ccccataata
    15781 cgcgtaatca cgttgatgcc ggtctggcgt aaaacccgta ccagccacgg tgccatgcgg
    15841 aacaatcccc agcaacataa agcgaacaat gcaatagcca caaagaaacc aaacagatag
    15901 ctaatgctgt gataacgcgt accccagacg atggtagaac tgatcgcccc tggccccgcc
    15961 atcaacggca acgccaatgg caccacacca atgctttcac gtaccgcggt ttctgatttt
    16021 tcttgcttgt tctgtttatc ctcgccaagc ttgccgctga tcatcgacat cgctatcgtc
    16081 accaccagga tgcccccggc gatacggaac gaatcaattg atataccaaa aagttgcaga
    16141 atcgtgtcgc cgagaaaaag cgagatccac aagataatgg ccacagacag gttggctgta
    16201 aggttagttt tgtttcgcgc tgccgctgtc tgataactgg tcatgctgat aaagacggga
    16261 ataatcccta ccgggttgac cagcgcaaat aacccgatga aaaatttgaa gtaaacggga
    16321 aaatcaaaaa aggtctgaat cacggttagc tccgaagcaa aagccggata atgttagcca
    16381 taaataaggt tgaaaagacg cgctgacaat acgccttttg acagcatttt tcacctccta
    16441 actacttaaa attgctatca ttcgttattg ttatctagtt gtgcaaaaca tgctaatgta
    16501 gccaccaaat catactacaa tttattaact gttagctata atggcgaaaa gcgatgctga
    16561 aaggtgtcag ctttgcaaaa atttgatttg gatcacgtaa tcggtaccca gaagtgagta
    16621 atcttgctta cgccacctgg aagtgacgca ttagagataa taactctaat gtttaaactc
    16681 ttttagtaaa tcacagtgag tgtgagcgcg agtaagcttt tgattttcat aggttaagca
    16741 aatcatcacc gcactgacta tactctcgta ttcgagcaga tgatttacta aaaaagttta
    16801 acattatcag gagagcatta tggctgttac taatgtcgct gaacttaacg cactcgtaga
    16861 gcgtgtaaaa aaagcccagc gtgaatatgc cagtttcact caagagcaag tagacaaaat
    16921 cttccgcgcc gccgctctgg ctgctgcaga tgctcgaatc ccactcgcga aaatggccgt
    16981 tgccgaatcc ggcatgggta tcgtcgaaga taaagtgatc aaaaaccact ttgcttctga
    17041 atatatctac aacgcctata aagatgaaaa aacctgtggt gttctgtctg aagacgacac
    17101 ttttggtacc atcactatcg ctgaaccaat cggtattatt tgcggtatcg ttccgaccac
    17161 taacccgact tcaactgcta tcttcaaatc gctgatcagt ctgaagaccc gtaacgccat
    17221 tatcttctcc ccgcacccgc gtgcaaaaga tgccaccaac aaagcggctg atatcgttct
    17281 gcaggctgct atcgctgccg gtgctccgaa agatctgatc ggctggatcg atcaaccttc
    17341 tgttgaactg tctaacgcac tgatgcacca cccagacatc aacctgatcc tcgcgactgg
    17401 tggtccgggc atggttaaag ccgcatacag ctccggtaaa ccagctatcg gtgtaggcgc
    17461 gggcaacact ccagttgtta tcgatgaaac tgctgatatc aaacgtgcag ttgcatctgt
    17521 actgatgtcc aaaaccttcg acaacggcgt aatctgtgct tctgaacagt ctgttgttgt
    17581 tgttgactct gtttatgacg ctgtacgtga acgttttgca acccacggcg gctatctgtt
    17641 gcagggtaaa gagctgaaag ctgttcagga tgttatcctg aaaaacggtg cgctgaacgc
    17701 ggctatcgtt ggtcagccag cctataaaat tgctgaactg gcaggcttct ctgtaccaga
    17761 aaacaccaag attctgatcg gtgaagtgac cgttgttgat gaaagcgaac cgttcgcaca
    17821 tgaaaaactg tccccgactc tggcaatgta ccgcgctaaa gatttcgaag acgcggtaga
    17881 aaaagcagag aaactggttg ctatgggcgg tatcggtcat acctcttgcc tgtacactga
    17941 ccaggataac caaccggctc gcgtttctta cttcggtcag aaaatgaaaa cggctcgtat
    18001 cctgattaac accccagcgt ctcagggtgg tatcggtgac ctgtataact tcaaactcgc
    18061 accttccctg actctgggtt gtggttcttg gggtggtaac tccatctctg aaaacgttgg
    18121 tccgaaacac ctgatcaaca agaaaaccgt tgctaagcga gctgaaaaca tgttgtggca
    18181 caaacttccg aaatctatct acttccgccg tggctccctg ccaatcgcgc tggatgaagt
    18241 gattactgat ggccacaaac gtgcgctcat cgtgactgac cgcttcctgt tcaacaatgg
    18301 ttatgctgat cagatcactt ccgtactgaa agcagcaggc gttgaaactg aagtcttctt
    18361 cgaagtagaa gcggacccga ccctgagcat cgttcgtaaa ggtgcagaac tggcaaactc
    18421 cttcaaacca gacgtgatta tcgcgctggg tggtggttcc ccgatggacg ccgcgaagat
    18481 catgtgggtt atgtacgaac atccggaaac tcacttcgaa gagctggcgc tgcgctttat
    18541 ggatatccgt aaacgtatct acaagttccc gaaaatgggc gtgaaagcga aaatgatcgc
    18601 tgtcaccacc acttctggta caggttctga agtcactccg tttgcggttg taactgacga
    18661 cgctactggt cagaaatatc cgctggcaga ctatgcgctg actccggata tggcgattgt
    18721 cgacgccaac ctggttatgg acatgccgaa gtccctgtgt gctttcggtg gtctggacgc
    18781 agtaactcac gccatggaag cttatgtttc tgtactggca tctgagttct ctgatggtca
    18841 ggctctgcag gcactgaaac tgctgaaaga atatctgcca gcgtcctacc acgaagggtc
    18901 taaaaatccg gtagcgcgtg aacgtgttca cagtgcagcg actatcgcgg gtatcgcgtt
    18961 tgcgaacgcc ttcctgggtg tatgtcactc aatggcgcac aaactgggtt cccagttcca
    19021 tattccgcac ggtctggcaa acgccctgct gatttgtaac gttattcgct acaatgcgaa
    19081 cgacaacccg accaagcaga ctgcattcag ccagtatgac cgtccgcagg ctcgccgtcg
    19141 ttatgctgaa attgccgacc acttgggtct gagcgcaccg ggcgaccgta ctgctgctaa
    19201 gatcgagaaa ctgctggcat ggctggaaac gctgaaggct gaactgggta ttccgaaatc
    19261 tatccgtgaa gctggcgttc aggaagcaga cttcctggcg aacgtggata aactgtctga
    19321 agatgcattc gatgaccagt gcaccggcgc taacccgcgt tacccgctga tctccgagct
    19381 gaaacagatt ctgctggata cctactacgg tcgtgattat gtagaaggtg aaactgcagc
    19441 gaagaaagaa gctgctccgg ctaaagctga gaaaaaagcg aaaaaatccg cttaatcagt
    19501 agcgctgtct ggcaacataa acggcccctt ctgggcaatg ccgatcagtt aaggattagt
    19561 tgaccgatcc ttaaactgag gcactataac ggcttccaca acagggagcc gttttcttat
    19621 gccacttctt aatgatctgc tcgatttcag tgaccatccg cttatgcctc cgccctctgc
    19681 acaactattt gcagaacacc ttcccaccga gtggatacaa cactgcctga cgctttctgc
    19741 tcatgcgacc gttcgccgcc gtcgtttacc gggggacatg gttatctgga tggtggtgca
    19801 atgagccaat taccgatgtt gttcgccgtc tgaacctgag cgcggatggc gaagcgggga
    19861 tgaacctgct ggcccgcagc gctgtcaccc aggcgcgtca gcgcgtgggg gccgccccag
    19921 tggaatggct cttccgccag accgcacaga caggggcgcg gaacgttacc tgaaggatga
    19981 ctggcacggc ctgcaacttt ttgccattga tggcgcacag ttcaggacac ctgataaacc
    20041 cgagctgcgt gaatattatg ggtctgccaa cacatccact aagcggcaga acgcctaccc
    20101 ggttatgcgt ctggtggcct taatgaacct gggaagtcac attctgctga atgccgtgac
    20161 cgcaccttac cggcagagcg aaactgtgct ggcccactcc atgctcgcca ccatcccgga
    20221 taactctatt acgttgtttg ataaactttt ttacagcgaa gacctgctgc tgacgctgaa
    20281 ccagaagggt tgtaaccgcc actggctact gcctgcgtgg aagaatatcg catcagaaat
    20341 gatagagctg ggaaacacgg cgtcaacagg gaccatcccg aaacgactgg agcatcttcg
    20401 gggtgctctg gaagtggtgt tcataacaaa acgcccccga ccatcaaggc cgaggtcggt
    20461 taagatatca aaaacccgtt atcctgtgaa acatagtgcc gctcctctta agtgaacggc
    20521 attgcccttc tggggccgta ttttttgttt acccaaagca acttttccat aaaccgacag
    20581 cattagcctt catcatattt gcgacgatgt ataacgccta aacacaggga tattgtactt
    20641 tacaggtcac aagtcaacgt cggtgcttaa gagccctgtg aggcgtatag cggcgttaaa
    20701 aaactgccga gaagggtata tagcccggaa gaagtgcgta aaacgaactg acaggataaa
    20761 agtgccctgc tcaccctgtc agtaaagaaa ttcttattaa tcgtggcgat gcctttcctg
    20821 aatagccgtt aatgagccga cttgtaacgc ctctttatag tgtttacggc atacagaaac
    20881 gtatcgttca ttaccaccaa ttaccacctg ctcaccttcg ttataaggtc tgcctgcttg
    20941 atcaagacgc agcaccatgc ttgctttacg gccacaaaaa cagatggttt ttaattcaac
    21001 cagtttgtcg gaccatgcca gtaagtattg gctgccaata aataattcac ctcgaaaatc
    21061 ggtacgtaaa ccataacaaa gtacgggtat atcgagttga tcgacaacct ccgataattc
    21121 atatacttgt tgtctggtta aaaactggca ttcatcaacc agtacgcaat gaattgcctg
    21181 ctgttcatgt tccgcacgaa tctcatcaaa taatgatgaa ttttggttaa ataattttgc
    21241 aggcgatgac aaacctatac gcgaactgac tttcccggca ccaaagcgat catcaatttc
    21301 tgccgtatat acgacagtgc gcatgccgcg ttcctggtaa ttgtatgaag attgcaacaa
    21361 tgctgtagac ttacccgcat tcattgcgga atagtagaaa tatagctgtg ccatcagcca
    21421 caggccctca atgatatgaa ataaagttgg ctggagttta tcataattcg tcagtttttc
    21481 agctcaacag tatgcacaga agtgggtata tgcgttctcc cttacgaagc cttgcataat
    21541 ccttctgagc tatcattaca actgcctcgc ttgttataag cgggtaaatg actgctggta
    21601 actattcaca atctttaacc tgttgcgcaa gtaatagccc tctgttgacc tccaggagat
    21661 agtgcaatac taagtccatg ctcttattgc gactgttcta cttttcatca ttcgcttaat
    21721 agggaattct cgtaaacaca actaatacag aagactgaaa ggtcgtcagc ctacgataat
    21781 ctccccataa aatgtgacat gaatcaggaa gttttaacct cacgtgctgc gaaatcatcg
    21841 gtgtaaatag ggctatatgc cgcgtctttt ctggctaatt ttatgaaaag atatttattg
    21901 gcggcacaaa ataaagaaca attttgaatt ccttacattc ctggctattg cacaactgaa
    21961 tttaaggctc tattattacc tcaacaaacc accccaatat aagtttgaga ttactacaat
    22021 gagcgaagca cttaaaattc tgaacaacat ccgtactctt cgtgcgcagg caagagaatg
    22081 tacacttgaa acgctggaag aaatgctgga aaaattagaa gttgttgtta acgaacgtcg
    22141 cgaagaagaa agcgcggctg ctgctgaagt tgaagagcgc actcgtaaac tgcagcaata
    22201 tcgcgaaatg ctgatcgctg acggtattga cccgaacgaa ctgctgaata gccttgcttc
    22261 cgttaaatct ggcaccaaag ctaaacgtgc tcagcgtccg gcaaaatata gctacgttga
    22321 cgaaaacggc gaaactaaaa cctggactgg ccaaggccgt actccagctg taatcaaaaa
    22381 agcaatggat gagcaaggta aatccctcga cgatttcctg atcaagcaat aatcttttgt
    22441 agattgcact tg
//
LOCUS       DAZGLJ010000055        22333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000055 DAZGLJ010000000
VERSION     DAZGLJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.055"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(38..199)
                     /gene="rsmS"
                     /locus_tag="ACHK49_001946"
     CDS             complement(38..199)
                     /gene="rsmS"
                     /locus_tag="ACHK49_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO9262600.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(213..740)
                     /gene="priC"
                     /locus_tag="ACHK49_001947"
     CDS             complement(213..740)
                     /gene="priC"
                     /locus_tag="ACHK49_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO9262601.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            810..1187
                     /gene="ybaN"
                     /locus_tag="ACHK49_001948"
     CDS             810..1187
                     /gene="ybaN"
                     /locus_tag="ACHK49_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO9262602.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            1340..1891
                     /gene="apt"
                     /locus_tag="ACHK49_001949"
     CDS             1340..1891
                     /gene="apt"
                     /locus_tag="ACHK49_001949"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO9262603.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            2020..3951
                     /gene="dnaX"
                     /locus_tag="ACHK49_001950"
     CDS             2020..3951
                     /gene="dnaX"
                     /locus_tag="ACHK49_001950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO9262604.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    3284..3348
                     /gene="dnaX"
                     /locus_tag="ACHK49_001950"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            4004..4333
                     /gene="ybaB"
                     /locus_tag="ACHK49_001951"
     CDS             4004..4333
                     /gene="ybaB"
                     /locus_tag="ACHK49_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO9262605.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            4333..4938
                     /gene="recR"
                     /locus_tag="ACHK49_001952"
     CDS             4333..4938
                     /gene="recR"
                     /locus_tag="ACHK49_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO9262606.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            5048..6922
                     /gene="htpG"
                     /locus_tag="ACHK49_001953"
     CDS             5048..6922
                     /gene="htpG"
                     /locus_tag="ACHK49_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO9262607.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVELEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDADKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            7103..7747
                     /gene="adk"
                     /locus_tag="ACHK49_001954"
     CDS             7103..7747
                     /gene="adk"
                     /locus_tag="ACHK49_001954"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO9262608.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            7983..8945
                     /gene="hemH"
                     /locus_tag="ACHK49_001955"
     CDS             7983..8945
                     /gene="hemH"
                     /locus_tag="ACHK49_001955"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO9262609.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKMYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(8942..9901)
                     /gene="aes"
                     /locus_tag="ACHK49_001956"
     CDS             complement(8942..9901)
                     /gene="aes"
                     /locus_tag="ACHK49_001956"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO9262610.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            10053..11357
                     /gene="gsk"
                     /locus_tag="ACHK49_001957"
     CDS             10053..11357
                     /gene="gsk"
                     /locus_tag="ACHK49_001957"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO9262611.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(11490..13166)
                     /gene="ybaL"
                     /locus_tag="ACHK49_001958"
     CDS             complement(11490..13166)
                     /gene="ybaL"
                     /locus_tag="ACHK49_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO9262612.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(13404..14624)
                     /gene="fsr"
                     /locus_tag="ACHK49_001959"
     CDS             complement(13404..14624)
                     /gene="fsr"
                     /locus_tag="ACHK49_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO9262613.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            14842..16494
                     /gene="ushA"
                     /locus_tag="ACHK49_001960"
     CDS             14842..16494
                     /gene="ushA"
                     /locus_tag="ACHK49_001960"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO9262614.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGKMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGRLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(16531..17010)
                     /gene="ybaK"
                     /locus_tag="ACHK49_001961"
     CDS             complement(16531..17010)
                     /gene="ybaK"
                     /locus_tag="ACHK49_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO9262615.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(17214..18008)
                     /gene="ybaP"
                     /locus_tag="ACHK49_001962"
     CDS             complement(17214..18008)
                     /gene="ybaP"
                     /locus_tag="ACHK49_001962"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO9262616.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            18146..18487
                     /gene="ybaQ"
                     /locus_tag="ACHK49_001963"
     CDS             18146..18487
                     /gene="ybaQ"
                     /locus_tag="ACHK49_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO9262617.1"
                     /translation="MKQATRKPTTPGDILLYEYLVPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(18601..21105)
                     /gene="copA"
                     /locus_tag="ACHK49_001964"
     CDS             complement(18601..21105)
                     /gene="copA"
                     /locus_tag="ACHK49_001964"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO9262618.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            21367..22299
                     /gene="glsA"
                     /locus_tag="ACHK49_001965"
     CDS             21367..22299
                     /gene="glsA"
                     /locus_tag="ACHK49_001965"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO9262619.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
BASE COUNT         5492 a         5966 c         5901 g         4974 t
ORIGIN      
        1 acaatcttag gggacattta gcggccccat ttatttctca cttttccgcc tcatcatcgc
       61 gcgttaattt cttttcataa tcgcgtttta caatatccag cgcgcgcagc acggtgcggg
      121 ctgggatctg attttcttcc agcagcacaa tcaaatcgac agccagtttg acatcgtcag
      181 gggcattttc cagtgacata ctctctccat tgctagcggg ttaagcgcgc tagcctgttt
      241 tcgatttttt ccagcgcatg gcggcagcgt gccaggcgcg cttcataggc ttccacttca
      301 cgatgcagcg tttgctgttc cacgagatcg gtcacccgcg ccaggcgggc tctgcgttcg
      361 gcaaccatct cacgtagccg ccgctcaaaa tcctgatgct gaatacgttt acgctgccag
      421 cgggcaattt tcggtggcgc actgtcccac tcgcgcaatg accaggcgga ggcttcacgc
      481 gcgatggctt ccagttgtgc cgccagatgt tccgccagcc aggccacttg cggcagctgt
      541 tgctgctcaa ctgcatgacg aagcgcagcc agattatcgc ccgcctcatc gagacaagcc
      601 tgcagtgttg tcgcacgggt ttgaaaaagg tgtctgtcaa aacgggcact tagcgtggca
      661 aattgcgcta ccggggcaca acgctgacgc agcgtagcga gctgtccttc cagtttttcc
      721 agcagcaggg cggttttcac gataagacct caatgaaaat gataattgtt atgctaaagt
      781 agccactctg taagctgaca acactcaata tgcaacgaat cattttaatc atcattggct
      841 ggctggcggt agtgctgggt acgctgggcg tggtattacc ggtattaccg acgacgccgt
      901 ttatcctgct ggcggcctgg tgctttgccc gttcttcccc gcgctttcac gcctggttgc
      961 tgtaccgctc atggtttggc agctatctac gtttctggca gaaacatcat gcgatgccgc
     1021 gcggcgtcaa accgcgggcg attttgctta ttttgctcac gtttgccatt tctctgtggt
     1081 tcgtccagat gccatgggtg cgcatcatgt tgctggtaat tctcgcctgt ttgcttttct
     1141 atatgtggcg aattccggtg attgatgaaa agcaagaaaa gcactgaagc acaacaatcg
     1201 cagttgcaat tattgcgtac agccagtaca ttctggcgtt ttcgagcaca ggcgcaggcg
     1261 gtcaaaggtt aaacaactgt tacttttgat acgtttaaaa cgcgccgtga gtaccaccgt
     1321 aacaagcagg catacactta tgaccgcgac tgcacagcag cttgagtatc tcaaaaatag
     1381 catcaaaagc attcaggact acccaaaacc cggcattctt ttccgcgatg tcaccagctt
     1441 actggaagac ccgaaagctt acgctctcag catcgacttg ctggttgagc gttacaaaaa
     1501 tgcgggcatt accaaagttg tcggcaccga agcgcgtggc ttcttgtttg gcgctccggt
     1561 agctctgggt ctgggcgttg gctttgtacc ggtccgtaaa ccgggcaaac tgccgcgtga
     1621 aaccatcagt gaaacttacg acctggaata cggcaccgat cagctggaga tccacgttga
     1681 tgccatcaaa ccgggcgaca aagttctggt ggtggacgat ctgctggcaa ccggcggcac
     1741 tatcgaagcg accgttaaac tgatccgtcg tctgggtggt gaagtggctg acgctgcgtt
     1801 cattatcaac ctgttcgatc tcggcggcga acagcgtctc gaaaaacagg gcattaccag
     1861 ctacagcctt gtcccgttcc cgggccatta attatcgcca gtcttgtgct gcccacgcta
     1921 cggacagcac aagatgtgca ttcagcctcg ccgttctgac ggggctgtgt tagcattacc
     1981 ccttcgtgaa tccaccttcc agcgtttcag agcctgccaa tgagttatca ggtcttagcc
     2041 cgaaaatggc gcccacaaac ctttgctgac gtcgtcggcc aggaacatgt gctgaccgca
     2101 ctggcgaacg gcttgtcgtt agggcgtatt catcatgctt atcttttttc cggtacccgt
     2161 ggcgtcggaa aaacctctat cgcccgactg ctggcgaagg ggctaaactg cgaaaccggc
     2221 attaccgcga cgccgtgcgg cgtgtgcgat aactgtcgtg aaatcgagca ggggcgcttt
     2281 gtcgatctga ttgaaatcga cgccgcctcg cgcaccaaag ttgaagatac ccgcgacctg
     2341 ctggataacg tccagtacgc tccggcgcgt ggtcgtttca aagtttatct gatcgacgaa
     2401 gtgcatatgc tgtcgcgcca cagctttaac gcactgttaa aaacccttga agagccgccg
     2461 gagcacgtta agtttctgct ggcgacgacc gatccacaga aattgccggt gacgattttg
     2521 tcgcgctgtt tgcaatttca tctcaaggcg ctggatgtcg agcaaattcg ccatcaactt
     2581 gagcacatcc tcaacgaaga acatatcgct cacgagccgc gggcgttgca attgctggcg
     2641 cgcgccgctg aaggcagcct gcgagatgcc ttaagtctga ccgaccaggc gattgccagc
     2701 ggtgacggcc aggtttcaac ccaggcagtc agtgcgatgc tgggtacgct tgacgacgat
     2761 caggcgctgt cgctggttga agcgatggtc gaggccaacg gcgagcgcgt aatggcgctg
     2821 attaatgaag ccgctgcccg tggtatcgag tgggaagcgt tgctggtgga aatgctcggc
     2881 ctgttgcatc gtattgcgat ggtacaactt tcgcctgctg cacttggcaa cgacatggcc
     2941 gccattgagc tgcggatgcg tgaactggcg cgcaccatac cgccgacgga tattcagctt
     3001 tactatcaga cgctgttgat tggtcgcaaa gaattaccgt atgcgccgga ccgccgcatg
     3061 ggcgttgaga tgacgctgct gcgcgcgctg gcgttccatc cacgtatgcc tctgcctgag
     3121 ccagaagtgc cacgccagtc ctttgcgccc gtcgcgccaa cggcagtaat gacgccaacc
     3181 caggtgccgc cgcaaccgca atcagcgccg cagcaggcac cgactgtacc gctcccggaa
     3241 accaccagcc aggtgctggc ggcgcgccag cagttgcagc gcgtgcaggg agcaaccaaa
     3301 gcaaaaaaga gtgaaccggc agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg
     3361 gaaagactgg cttcggtcac cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa
     3421 aaagcgccag ccaaaaaaga agcgtatcgc tggaaggcta ccactccagt gatgcagcaa
     3481 aaagaagtgg tcgccacgcc gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg
     3541 gaactggcgg cgaagctggc ggcagaagcc attgagcgcg acgcgtgggc ggcacaggtg
     3601 agtcaacttt cgctaccaaa actggtcgaa caggtggcct taaatgcctg gaaagaggag
     3661 agcgacaacg cagtatgtct gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt
     3721 gcacagcaaa aactggctga agcgttgagc acgttaaaag gttcaacggt tgaactgact
     3781 atcgttgaag atgataatcc cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac
     3841 gaagaaaaac ttgcgcaggc gcgcgagtcc attattgcgg ataataatat tcagaccctg
     3901 cgtcggttct tcgatgcgga gctggatgaa gaaagtatcc gccccatttg atcgtaagca
     3961 cagcttacgt tcgtcatcct taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg
     4021 tctgggtaac ctgatgaagc aagcccagca gatgcaagaa aaaatgcaga aaatgcagga
     4081 agagatcgcg cagctggaag tcaccggcga atctggcgca ggtctggtaa aagtgaccat
     4141 caacggtgca cacaactgcc gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa
     4201 agagatgctg gaagacctgg tggctgcagc attcaacgac gcagcacgtc gtattgaaga
     4261 aacgcagaaa gaaaaaatgg cctctgtatc ctccggaatg cagctgccgc ctggctttaa
     4321 gatgccgttc tgatgcaaac cagcccgctg ttaacacagc ttatggaagc actgcgctgt
     4381 ctgccgggcg ttggcccgaa gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat
     4441 cgtagcggcg ggatgcgtct ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac
     4501 tgcgccgatt gccgcacttt caccgaacag gaagtctgta acatctgttc gaatccgcgt
     4561 cgtcaggaaa acggtcaaat ctgtgtggtg gagagtccgg cggacatcta cgccattgag
     4621 cagacggggc agttttcagg ccgttatttt gtgttgatgg ggcacctgtc accgctggac
     4681 ggcatcggtc cggatgatat cggccttgat cgtctggaac agcgtctggc agaggaaaaa
     4741 atcactgaag tgatcctcgc caccaatccc acggttgaag gtgaagctac cgctaactac
     4801 attgccgagc tttgcgcgca atatgacgtg gaagccagcc gaatcgctca tggcgtaccg
     4861 gttggcggcg aactggaaat ggtcgatggc accacgctgt cacactccct tgccgggcgt
     4921 cataagattc gtttttaagc aaacgagagc aggatcacct gctctcgctt gaaattattc
     4981 tcccttgtcc ccatctctcc ctcatcctgt ttttaacctt aaaatggcat tattgaggta
     5041 gacctacatg aaaggacaag aaactcgtgg ttttcagtca gaagtgaaac agcttctgca
     5101 cctgatgatc cattctctct attccaataa agaaatcttc ctgcgtgagc ttatctctaa
     5161 cgcctccgat gcggcggaca agctgcgttt ccgtgcgcta tctaacccgg acctgtacga
     5221 aggtgatggc gaactgcgcg ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat
     5281 ctccgataac ggcgtgggga tgacccgcga cgaagtgatt gaccatctgg ggactatcgc
     5341 taaatccggt accaaatcat tcctcgaatc cctgggttct gaccaggcga aagacagcca
     5401 gctgatcggt cagtttggtg ttggtttcta ctctgcgttt atcgtggccg acaaagtgac
     5461 cgtgcgtact cgcgcggcag gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg
     5521 cgaaggtgaa tataccgttg ccgacatcac caaagaagat cgtggtactg aaatcaccct
     5581 gcatctgcgt gaaggcgaag acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag
     5641 caaatactcc gaccatatcg cgctgccggt agagctcgaa aaacgcgaag agaaagacgg
     5701 cgaaaccgtt atctcctggg agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa
     5761 gtcggaaatc accgatgaag agtacaaaga gttctacaaa cacatcgccc acgactttaa
     5821 tgatccgctg acctggagcc acaaccgcgt tgaaggtaag caggagtaca ccagcctgct
     5881 gtacatcccg tcccaggctc cgtgggatat gtggaaccgt gatcataaac acggcctgaa
     5941 actgtacgtc cagcgtgtgt tcatcatgga cgacgcagaa cagttcatgc cgaactatct
     6001 gcgcttcgtg cgtggtctga ttgactccag cgatctgccg ctgaacgttt cccgtgaaat
     6061 cctccaggac agcacggtaa cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca
     6121 aatgctggaa aaactggcga aagacgacgc ggacaaatac cagaccttct ggcaacagtt
     6181 tggcctggta ctgaaagaag gtccggcgga agatttcgct aaccaggaag cgatcgccaa
     6241 actgctgcgt tttgcttcta cttataccga ttcttctgcg cagaccgtgt ctctggaaga
     6301 ctacgtttcc cgcatgaaag aagggcagga gaaaatctac tacatcactg cagacagcta
     6361 tgcggcagcg aagagcagcc cgcacctgga actgctgcgt aagaaaggca tcgaagttct
     6421 gctgctttcc gaccgcatcg atgagtggat gatgaactat ctgactgagt tcgacggtaa
     6481 accgttccag tcggtgtcta aagttgacga gtcgctggaa aaactggctg acgaagttga
     6541 tgagagcgcg aaagaagcgg agaaagcact gactccgttc atcgaccgtg tgaaagccct
     6601 gctcggcgag cgcgtgaaag atgtccgtct gactcaccgt ctgaccgata cgccagcgat
     6661 cgtttcgacc gacgcggacg aaatgagcac tcagatggcg aaactgttcg ctgcggcggg
     6721 ccagaaagtg ccagaagtga aatacatctt cgaactgaac ccggatcacg tactggtgaa
     6781 acgtgcggca gatactgaag atgaagcgaa gttcagcgag tgggtagaac tgctgctgga
     6841 tcaggcgctg ctggcagaac gcggcacgct ggaagatccg aacctgttta ttcgtcgtat
     6901 gaaccagctg ctggtttcct gatgtcatgc cggatgacct ttgtgtcatc cggcattttt
     6961 cttttcatca tctgcacttt ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg
     7021 cctttcttga ggcaatcgcc tgttggtggt atcgtttatc gctttttcaa aaaattcgac
     7081 acattttaag gggattttcg caatgcgtat cattctgctt ggcgctccgg gcgcggggaa
     7141 agggactcag gctcagttca tcatggagaa atatggtatt ccgcaaatct ccactggcga
     7201 tatgctgcgt gctgcggtca aatctggctc cgagctgggt aaacaagcaa aagacattat
     7261 ggatgctggc aaattggtca ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc
     7321 tcaggaagac tgccgtaatg gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc
     7381 agacgcgatg aaagaagcgg gcatcaatgt tgattacgtt ctggaattcg acgtaccgga
     7441 cgaactgatt gttgatcgta tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta
     7501 tcacgttaaa ttcaatccgc cgaaagtaga aggcaaagac gacgttaccg gtgaagaact
     7561 gactacccgt aaagacgatc aggaagaaac cgtgcgtaaa cgtctggttg aataccatca
     7621 gatgactgca ccgctgatcg gctactactc caaagaagcg gaagcgggta acaccaaata
     7681 cgcgaaagtt gacggcacca agccggttgc tgaagttcgc gctgatctgg aaaaaatcct
     7741 cggctaattc gaaagcgcgc acggacagtc ccctcgcccc ctcggggaga gggttagggt
     7801 gaggggaaca ggcccgcaca agcaaactta tcagcaatct caggccggat attcattcgg
     7861 ccttttacaa aaacctcaat ctgcgctatt cctttttctg atttgacctc tcacaacaat
     7921 tagttcttct tcctcacttt tccgctacaa ttatcaacaa gttgaatcga taagaggcgg
     7981 taatgcgtca gactaaaacc ggtatcctgc tggcaaacct gggtacgccc gatgccccca
     8041 cacctgaagc ggtaaaacgc tatctgaaac aatttttaag cgacagacgc gtggttgata
     8101 cctcacggtt gttatggtgg ccgttgctgc gcggcgtgat tttgccgctg cgctcgccgc
     8161 gtgtggcgaa gatgtacgcc tcggtctgga tggaagacgg ctcgccgctg atggtttaca
     8221 gccgccagca acagcaggcg ttggcacaac gtttaccgga tacgcccgta gcgctgggca
     8281 tgagctacgg ctcgccatca ctggaaagcg ccgtcgatga actcctggca gagcatgtag
     8341 atcatattgt ggtgctgccg ctttatccgc aattctcctg ttctacggtc ggtgcggtat
     8401 gggatgaact ggcacgcatt ctggcgcgca aacgtagcat tccggggata tcgtttattc
     8461 gtgattacgc cgataaccac gattacatta atgcactggc gaacagcgta cgcgcttctt
     8521 ttgccaaaca tggcgaaccg gatctgctgc tgctctctta tcatggcatt ccccagcgtt
     8581 atgcagatga aggcgatgat tacccgcaac gttgccgcac aacgactcgc gaactggctt
     8641 ccgcactggg gatggcaccg gaaaaagtga tgatgacctt tcagtcgcgc tttggccggg
     8701 aaccctggct gatgccttat accgacgaaa cgctgaaaat gctcggagaa aaaggcgtag
     8761 gtcatataca ggtgatgtgc ccgggctttg ctgcggattg tctggagacg ctggaagaga
     8821 ttgccgagca aaaccgtgag gtcttcctcg gtgccggcgg gaaaaaatat gaatatattc
     8881 cagcgcttaa tgccacgccg gaacatatcg aaatgatggc taatcttgtt gccgcgtatc
     8941 gctaaagctg agcggtaaag aactgagcgc cgtcgcgaag agcctcgtcg gcggttttca
     9001 tcattcgtga ataatgcaaa aaggcatgca gcgtacctgg gtagagtttg aactcacagg
     9061 gctgctgatg cgccgctaac gtctggtaaa gcagacggct gtcatccagc agcggatcga
     9121 actccgcccc ggcaataaaa cagggcggaa cttcgcgagt gagatcatta ttaaacagac
     9181 agtaatacgg tgactcgcgg tccgcgtcgt tgcttaaata cgcctcttca tacatctgca
     9241 aatcctgttg cgttaagcca tcccagacac cgcccaacag acgacgagtc acggaatccc
     9301 gtaatccgta aagcccatac cacagcaaaa cacccgcaat tttaccgcaa tcgatctgtt
     9361 tatcacgcaa ccacaacgca ctggcgagcg ccagcatggc acctgcggaa tcaccggcaa
     9421 agccaatgcg ggacatattg atttgataat cctccgcctg ctggtggaaa taacaacaag
     9481 cagccacaat ttcctctatc gcttgcggaa aacgcgcttc aggtgaaagg gggtaatcaa
     9541 taccaatcac cgtacattgg ctgtagcttg ccagcaggcg catgatgcga tcgtgggtat
     9601 cgagattgcc gagaataaaa ccgcctccat gcaaataaaa tagcgtcgcc gggctatctg
     9661 gctgcggaca aaagagacgt gtttccacct gcccatattt tgttggaacc atgtaagctc
     9721 tggttgccat ttctggagcg cccgcattcc agaatcggcg ctcaagcgtg taatactgtc
     9781 gttgctcagc aatcgttccc gttgcgggcc aggacggtaa atccggctga agagtattca
     9841 caacggtctt catttcagca gaaataaggt ccagaacagg tagtttgttt tccggcttca
     9901 tacaaaaact cctttcaaat tacgtcattg taaggaacca ctgccatgaa aatgcgatcc
     9961 cgcctgctga tattgaaact ggctgcgtct cgcgcgctcc cgtcagattg tgttaacatt
    10021 cgccgctcag ttaaccaccc gtaaaaacaa ccatgaaatt tcccggtaaa cgtaaatcca
    10081 aacattactt ccctgtaaat gcacgcgatc cgctgcttca gcaattccag ccagaaaacg
    10141 aaaccagcgc cgcctgggtc gtaggtattg accagacgct ggtcgatatt gaagcgaaag
    10201 tggatgatga attcattgag cgttatggat taagcgccgg gcattcactg gtgattgagg
    10261 atgacgtagc cgaagcgctt tatcaggagc taaaacagaa aaacctgatt acccatcagt
    10321 ttgcgggtgg caccattggt aacaccatgc acaactactc ggtgctcgcg gacgaccgtt
    10381 cggtgctgct gggcgtcatg tgcagcaata ttgaaattgg cagttatgcc tatcgttacc
    10441 tgtgtaacac ttccagccgt accgatctta actatctaca aggcgtggat ggcccgattg
    10501 gtcgttgctt tacgctgatt ggcgagtccg gggaacgtac ctttgctatc agtccaggcc
    10561 acatgaacca gctgcgggct gaaagcattc cggaagatgt gattgccgga gcctcggcac
    10621 tggttctcac ctcatatctg gtgcgttgca agccgggtga acccatgccg gaagcaacca
    10681 tgaaagccat tgagtacgcg aagaaatata acgtaccggt ggtgctgacg ctgggcacca
    10741 agtttgtcat tgccgagaat ccgcagtggt ggcagcaatt cctcaaagac cacgtttcta
    10801 tccttgcgat gaacgaagat gaagccgaag cgttgaccgg agaaagcgat ccgttgttgg
    10861 catctgacaa ggcgctggac tgggtagatc tggtgctgtg caccgccggg ccaatcggct
    10921 tgtatatggc gggctttacc gaagacgaag cgaaacgtaa aacccagcat ccgctgctgc
    10981 cgggcgctat agcggaattc aaccagtatg agtttagccg cgccatgcgc cacaaggatt
    11041 gccagaatcc gctgcgtgta tattcgcaca ttgcgccgta catgggcggg ccggaaaaaa
    11101 tcatgaacac taatggagcg ggggatggcg cattggcagc gttgctgcat gacattaccg
    11161 ccaacagcta ccatcgtagc aacgtaccaa actccagcaa acataaattc acctggttaa
    11221 cttattcatc gttagcgcag gtgtgtaaat atgctaaccg tgtgagctat caggtactga
    11281 accagcattc acctcgttta acgcgcggct tgccggagcg tgaagacagc ctggaagagt
    11341 cttactggga tcgttaagtt atcgtcggtt cgtaggccag ataaggcgtt cacgccgcat
    11401 ctggcatttg gctctcgatg cctgatgcga cgctggcgcg tcttatcatg cctacatatt
    11461 tttcatattt tacatccggc aaccaccgtt taccccgtca ccacctcacc cgccggtggc
    11521 gtttccagca gttccagcat ggtacgggcg atttcacgct cgcccatcac tacctgattc
    11581 gcaccacgtt cggtgatata cgccacttca tcgtcataat gggcgcgggc aataatctca
    11641 atatccggat ttttcgcgcg ggcagatgcc acaatctcac ccgcttcata accgttggga
    11701 atcgtcagga tcagccattt tgcacattcc agatgcgcca gttgcataat ttcttcgttc
    11761 gccgcattgc ccaatactgc gcggaccccg cgctctcgca gctcatcaac acgggttcgt
    11821 gacgtctcaa tcaccaccag cggaatatca gaggcgagca atttctcccc cagcaggctg
    11881 cctacacgac cgtaacccac cagtagcgca tggttgcaaa tatccactgg gatctgcttc
    11941 tcttcttcga ttgcctcttc cagcgtctgc tcttccagcg tttcggtctt cgccagatat
    12001 ttctccagta gtgcgaacag taccgggttg agcataatcg acaggatcgc ccctgccagt
    12061 actaggtttt gtccggcctg cggcagtaaa ttcaatgcca ttcccagtcc cgccaggata
    12121 aacgcgaact caccaatctg cgccaggctg gcggcgatgg ttaatgccgt acgttgggag
    12181 tgaccaaaca gtcgcaccag gaaaaatgcg gctaacgact taccaaacag aataatcgcc
    12241 agcgtcgcca gcactgccag cggttgctga atcagaatta acggatcaaa caacatcccg
    12301 acggagacaa aaaacagcac cgcaaacgcg tcgcgcaatg gcagcgtatc gtgggcggca
    12361 cggtgactca gttcagactc gttcagtacc atcccggcaa agaacgcacc gagtgcaaag
    12421 gagacatcaa acagctctac cgcaccaaag gcaatcccta acgccagcgc cagcaccgac
    12481 agggtaaaca gctcgcgaga accggttgcc gcgctgcgtg ccataatcca cggcaccaga
    12541 cggcgaccta ccagcatcat aatggcgata aatgcgatca ctttgccgat ggtgatcccc
    12601 atatcgactg caagagtggc aaagcccaca tcgccctgtt ccatcattcc tgccactgcg
    12661 ggcagcaaca ccagcgtcag aaccattacc aggtcttcca caatcaacca accgatggcg
    12721 atttgcccac gctgactgtc aattaattgc cgttcttcaa gtgcgcgcag taacaccacg
    12781 gtactggcgg tggaaagaca taaaccgaac acgataccgg tcattaacga ccagcccagc
    12841 acggcggaga gcgccatacc cagcagcgtc gccacggcta tctgggcgat cgcgccggga
    12901 atggcgatgg actttaccgc catcaaatcc ttgagcgaaa aatgcaaacc gacgccgaac
    12961 atcagcaaaa taacgcccag ttcagccagt tccggcgcaa gcttggtatc ggcaacaaag
    13021 cccggagtga atggtcctgc cagcacaccc gctaacagat atcccaccag aggagaaata
    13081 cgtagtttat tggccagcat gccgaggata aaggcgagca caaggccgcc aacaatggtg
    13141 gtgataagcg gggtggcgtg atgcattccg tctccttttc ctggtggtta ttgtccattt
    13201 ttggccggga aaaccaaaat tacaggtaat agtttatgac aatttcattg atgatgttca
    13261 tgaataatta ttgaattttg cagaaaaatg gaattagctg caaaaaaagc acggatcgga
    13321 aaacagaagg cggtttaaca gaggaaaggc ttatggcgac tgtaaaacga tgcagccaaa
    13381 gtttgccttt ggctgcaatg aaatcagtct ttatgccggt tatcaggcag gaatatggtc
    13441 aacatcccca atagtggcag gaaagcacag attttataga ctaactcgat actggtgtga
    13501 tcggcgataa gccccagaac tgccgctccc agacctccca taccaaaagc aaaaccgaaa
    13561 aagagtccag aaaccatacc gatacgtccc ggaagcagct cctgagcgta aaccagaatg
    13621 gcagagaatg ccgaagcgag gataaatcca ataatcaccg ttaaaacccc cgtccagtgc
    13681 aggctggcgt agggtaaaat cagcgtaaac ggcgcaacgc cgaggataga gccccaaatc
    13741 acatatttcc gcccaatttt atcccctaca ggcccgccga tcaccgtacc tgccgcaacg
    13801 gcaaacagga aggcaaacag atgaagctga gcattctgga tagataatcc gaatttttgc
    13861 atcagataaa aggtgtaata gctgctgatg ctcgccatat agaaatattt cgagaaaatg
    13921 aggattaaca gaatgctgac cgccagtacc actttgttgc gcggcagtgg attgataatc
    13981 gtcgctttgg gttttccttt attcattcgg tgctgtgccg agtaccaacg gctgatttgc
    14041 gccaacacca cgatcgccag cagtgccgca agcacaaacc aggcaacgtt gcctttgcca
    14101 taaggcgcga taatcaccgc cgccagcaag ggtcccaggg aactgccaaa gttgccgccg
    14161 acctgaaaga tagattgcgc caggccatgc cgcccgccgg aagccatacg ggctacgcga
    14221 gaagattccg gatgaaagac cgatgaaccg gtaccgacca gcgccgccgc cagcagaact
    14281 gcgccaaaac tgcccgccag cgcaagcagc accagaccac ttaaggtaaa gcacatgcca
    14341 attggcaacg accacggcat cggatattta tcggtccagt agccgaccac tggttgcagt
    14401 agcgaagagg cgagctggaa ggtgagggtt atcatgccaa tctgcataaa tgtcagagaa
    14461 aattctgact gaagcagcgg ataaatcgcc agaatcagcg attggatcat gtcgttcagc
    14521 agatgtgaga ggctgatagc acctaaaata ccaaacgatg ttcgggcctt ggtcgttgac
    14581 gcagccgcgc ccgccacagg ctggggttgt tcactcattg ccataggaaa gtcacttttt
    14641 cagggttgcg atgtaaagaa tgatcttatt tgtgattatt acccgactaa catacctgta
    14701 tgcgtcgcct gaaggaagtc tcaacgccga atacagaatt tctaatctgg atgcagattt
    14761 atcttcaccg gacgcagact tgtctatgat gtcgcgtcat actatttttc aacacgttga
    14821 aatcaggtca gggagagaag tatgaaatta ttgcagcggg gcgtggcgtt agcgctgtta
    14881 accacattta cactggcgag tgaaactgct ctggcgtatg agcaggataa aacctacaaa
    14941 attacagttc tgcataccaa tgatcatcat gggcattttt ggcgcaatga atatggcgaa
    15001 tatggtctgg cggcgcaaaa aacgctggtg gatggtatcc gcaaagaggt tgcggctgaa
    15061 ggcggtagcg tgctgctact ttccggtggc gacattaaca ctggcgtgcc cgagtctgac
    15121 ttacaggatg ccgaacctga ttttcgcggt atgaatctgg tgggctatga cgcgatggcg
    15181 atcggtaatc atgaatttga taatccgctc accgtattac gccagcagga aaagtgggcc
    15241 aagttcccgt tgctttccgc gaatatctac cagaaaagta ctggcgagcg cctgtttaaa
    15301 ccgtgggcgc tgtttaagcg tcaggatctg aaaattgccg tcattgggct gacaaccgat
    15361 gacacagcaa aaattggtaa cccggaatac ttcactgatg tcgaatttcg taagcccgcc
    15421 gatgaagcga agctggtgat tcaggagctg caacagacag aaaagccaga cattattatc
    15481 gcggcgaccc atatggggca ttacgataat ggtgagcacg gctctaacgc accgggcgat
    15541 gtggagatgg cacgcgcgct gcctgccgga tcgctggcga tgatcgtcgg tggtcactcg
    15601 caagatccgg tctgcatggc ggcagaaaac aaaaaacagg tcgattacgt gccgggtacg
    15661 ccatgcaagc cagatcaaca aaacggcatc tggattgtgc aggcgcatga gtggggcaaa
    15721 tacgtgggac gggctgattt tgagtttcgt aatggcaaaa tgaaaatggt taactaccag
    15781 ctgattccgg tgaacctgaa gaagaaagtg acctgggaag acgggaaaag cgagcgcgtg
    15841 ctttacactc ctgaaatcgc tgaaaaccag caaatgatct cgctgttatc accgttccag
    15901 aacaaaggca aagcgcagct ggaagtgaaa ataggcgaaa ccaatggtcg tctggaaggc
    15961 gatcgtgaca aagtgcgttt tgtacagacc aatatggggc ggttgattct ggcagcccaa
    16021 atggatcgca ctggtgccga ctttgcggtg atgagcggag gcggaattcg tgattctatc
    16081 gaagcaggcg atatcagcta taaaaacgtg ctgaaagtgc agccattcgg caatgtggtg
    16141 gtgtatgccg acatgaccgg taaagaggtg attgattacc tgaccgccgt cgcgcagatg
    16201 aagccagatt caggtgccta cccgcaattt gccaacgtta gctttgtggc gaaagacggc
    16261 agactgaacg accttaaaat caaaggcgaa ccggtcgatc cggcgaaaac ttaccgtatg
    16321 gcgacattaa acttcaatgc caccggcggt gatggatatc cgcgccttga taacaaaccg
    16381 ggctatgtga ataccggctt tattgatgcc gaagtgctga aagcgtatat ccagaaaagc
    16441 tcgccgctgg atgtgagtgt ttatgaaccg aaaggtgagg tgagctggca gtaatccgaa
    16501 agtgccggat gtttgcatcc ggcacaatgc ttaatcgcgg cgggcgatat cagcaaattt
    16561 ggcatcgagg atctttgcca gatcgcctgc cgccagttcg atatccagtc cgcgcttgcc
    16621 gccggaaaca taaatagtgg caaattcttg tgcgggggcg tcgataatcg ttggcagacg
    16681 ttttttctgc cccagtgggc taatcccccc aaccaggtat cccgtcgaac gctgcgcgac
    16741 catcggatcg gccatctcaa ctttcttggc acccagcgct tttgctactt ttttaagatc
    16801 cagttgaccg gcgaccggcg taacggccac ggcaaggtgt ttcatatcac cgttcactgc
    16861 caccagcagc gttttgtaga cctgatccgg attcaaacct aattttttga cgacttcatc
    16921 gccaaaattg gtttcagccg gatcgtgctc gtaggtatgg atttgaaacg aaatcttgtt
    16981 tttttcgagt aatttaactg cgggtgtcat ggtaattttc ctgccttaaa caaaaaaacg
    17041 aagcaagcat acgcctgatg gcggtccaaa aaaaagagtc atcttgccta agagtattgg
    17101 caggatggtg agattgagcg acaatcgagt tacaccgtcg cttaaagtga cggcataata
    17161 ataaaaaaat gaaattcctc tttgacgggc caatagcgat attggccatt tttttagcgc
    17221 aacatttgcg gtaaattccc ttctccatac aggtgtagtg caccgaccgc gaccacatat
    17281 cgccccggcg gcatggcgcg taatttatcc cgccaggcga gatttcgctg atgcatcagc
    17341 acatcgtaca gcgactgact gaacgtattg ggcagcgtta tttcattatt ttgcggcggt
    17401 gcattcagcc accagctcat catttgttgc agcaaccgtg cattggtgtg ccagtgggtc
    17461 agtgtatcgt ccagcagcgc cagtcctttg tcagggagct ggagcaacat ggcaatctgg
    17521 ttttcagccc cttccagttc aatcacgggt ttatgttgtt gcttcgccgc ctgcaatagc
    17581 tggtaatcga taccgtattc tgcccgcagc cccagttttt gtgcctgcgt cgcctgaaga
    17641 accatcgcga tttgccacag cggttgggta gaaaagagtg agggagaaat gcccatctcc
    17701 tggctaatgt gctgcagatt ttggagttgc tcctcgctaa tgcgctcttc cagcgcctcg
    17761 caggcaggca aattggcaaa aggcgtatcg ctggtggaaa catccgcctc gacgatcagc
    17821 gcatcggcgt ttttgagctt tttgagcaaa cgggtgggca ggggagccat atcgtggcta
    17881 cccatatgaa tactgccaat cagatgaaaa tggcgattgc cgggaagggt gatatcgatg
    17941 gcaggccagg tgtaatgatt accgcgcagc gcggcccaaa gcgtttttac ccggtacaac
    18001 agatccatac gacctccctt tgtgaaatat catgctagcg cgcggggagg gatggtgcaa
    18061 atggagttgg ggacttgttt aatgtgtttg tatgattcag tatattcttg tatcgctatt
    18121 cacagggaag caacagttaa aaactatgaa acaggcaaca agaaaaccga cgacacccgg
    18181 agatattctt ctctatgaat atctggtacc gctcgatttg aaaatcaatg agttagcaga
    18241 gttgctgcat gttcatcgta atagcgtcag tgcactgatc aataacaatc gtaaactcac
    18301 cactgagatg gcattccgtc tggcgaaagt ttttgatacc acagtcgatt tttggctaaa
    18361 cctccaggcg gcagttgatc tttgggaagt tgaaaacaac atgcgcaccc aggaagaatt
    18421 gggacggatt gaaacagtgg ctgaatactt ggcacgccgt gaagagcgtg caaaaaaggt
    18481 cgcgtaaggc caaaacatcg cttcatctcg taggccggat aaggcgttta cgccgcatcc
    18541 ggcagtcatg cgtcgatgcc aaatgtgcca ccctaaagca gcgcatccgc aatgacatac
    18601 ttattccttc ggtttaaacc gcagcaaccg gttggcgtta ctcactacgg taatcgacga
    18661 gagcgccatt gccgctccgg caactaccgg gttaagcagt gttccagtaa acggccacaa
    18721 aataccggcg gcgaccggaa taccgatact gttgtagata aaggcaccga gcaggttctg
    18781 cttcatgttg cgcagcgttg cgcgggaaat cgcgagcgca tccgcaacgc ccatcaggct
    18841 atggcgcatc agggtaatcg ccgcggtttc aatggcaaca tcactgccgc cccccatcgc
    18901 aataccgaca tccgcctggg ccagcgctgg cgcatcgtta atgccgtcgc ccaccattgc
    18961 cacctgacgt ccttcacttt gcaggcgttt aatcgcttcg gctttaccgt ccggcagcac
    19021 cccggcgatc acttcatcaa tcccggcttc tttggcgatc gcattggcgg tggttgggtt
    19081 atccccggtc aacatcacca gacgatatcc cgctttatgc aggcgttgca gcgccgccac
    19141 gctatcacta cgcaatggat cgcgtactgc cagcagggct accgctttcc cgtcaaccgc
    19201 cagcagcaca ggcgttgccc cttgcgatgc ctgagcagta atctccgctt cgatagcttt
    19261 ggtaccaacc tgttgctcat ttaacagcgc ctgattgccc agcaataacg catgaccttc
    19321 agcttcaccg ctcacgccca gcccgcgcaa tgtgcggaaa ccgttgacct gcggcagctg
    19381 catatcacct gctttatcga ggatcgcccg tgccagcgga tggctggaac cttgctccag
    19441 tgccgccgcc agacgcaatg cctgcgcttc atcaacatca gcaaacgttt tcactgcgac
    19501 aacctgcggc ttcccttcgg tcagcgtccc ggttttatcg aacacgacgg tatcgagtgt
    19561 actggcgcgt tgcagcgcgt cggcgtcccg caccagcacg ccaaactcag ccgcccgccc
    19621 gacgccggaa ataatcgaca tcggcgtcgc cagccccagc gcacacggac aggcgataat
    19681 cagtaccgtg gtggcaatca ccagggtata aacaatttgc ggtgccggac caaagaagta
    19741 ccagattgcc gcactgacca gcgcaatcac caccaccaca ggtacaaata cggctgagat
    19801 tttatccgcc agctgaccga tttctggctt gctgctctgg gcctggcgca ccatgcgaat
    19861 gatccgtgac agcgtggtat ggctgccaac cgcactggca cgaaacagca cgctaccgtc
    19921 ctgtaccact gtcccggcat ggacgctttc gccttcgcct ttttgctgcg ggattggttc
    19981 gcccgtcagc atcgcttcat ccagccatgc ttcgccctgg gtaatctcgc catctaccgg
    20041 cacgcgatcg ccggtcgtca ggcgcagcaa catacctggc tgcacttccg ccagaggcac
    20101 gcttttttca ccttcgtcag taaccaggcg cgccgtcggt ggggttaaat cgagtaattt
    20161 ctccagcgcc ttagaagaac gctgacgtgc gcgtgcttcc agcatatggc cgagattgat
    20221 cagaccgata atcatcgcgc tggcttcgta ataaagatgt cgcgcttcca tcgggaacca
    20281 ctgcggccac aggttgacgc tcatcgaata gagccacgcc acgccagtac ccagcgccac
    20341 cagcgtatcc atcgtcgctg caccgttcag caggcttttc catgcactgc ggtaaaaatg
    20401 gccgccggcg aaaaccatca ccgccagggt tatcaggccg ataaccaacc acaggctgcg
    20461 gttgtcagcg gtgaccatca tgttatcgcc aatcatcccc cagaccatca ccgggatacc
    20521 caccgccagt gcgacaattg cctgccagcg gaagcgcttc atcgtagcaa cggcggtttc
    20581 ctgctggcgc tcacggcgtt tcgcgtcatc ttcaatcgct tccgcgccgt agcccgcttt
    20641 ttccaccgcc tgcactaaat cttgtgggga ggcactgccc atcaccagcg cagtacgctc
    20701 cgccaggttt acccgtgcct gagtgacgcc cggtacgctt tgcagcgcat tttgtacgcg
    20761 ggtgacacag ctggcgcagc tcatgccgct cagcagcaac tgctggctgt catcgtcatc
    20821 ggcggtcgct gccggaagcg cctcagaaac cgctgtcagt gcttccgacg ggattgatga
    20881 ctccgccagc ggtttagcct ttgggtggct tacagatgcg tcataacccg cttgtttgat
    20941 ggtttcgatc agctgttctg cactggcagt cccggtaacg tgcgcttcag tgatagacac
    21001 atccgcttgt tccacatccg ggcgctgttc gagactttct ttcacgcgtt taacgcagtg
    21061 accgcaggac aggccgtcca gggtcaggtc gataatttgt gacataaaac actcctttaa
    21121 gacagttttg actggctgtg ataaaggtta aaccttccag caaggggaag gtcaagaaat
    21181 taataaacca ggcgagtaaa agtccgtaaa gattaaaaaa ttggctcaat ttgcatcagg
    21241 attagacatt tatctctttg ttttcctgta gttaagttgc gggtgctaag ttaaatcaag
    21301 atgcctgaaa atcggcaccg gggtgaggaa ttacctcccg catctataaa aaggagttaa
    21361 caaaagatgt tagatgcaaa caaattacag caggcagtgg atcaggctta cacccaattt
    21421 cactcactta acggcggaca aaatgccgac tacattccct ttctggcgaa tgtacctagt
    21481 caactggcag cagtggctat tgtgacctgc gatggcaaca tctatcgcgc gggtgatagt
    21541 gattaccgct ttgcgctgga atccatctcg aaagtctgta cgttagccct tgcgttagaa
    21601 gatgtcggcc cgcaggcggt acaggacaaa attggcgctg acccgaccgg attgcccttt
    21661 aactcagtta tcgccttaga gttgcatggc ggcaaaccac tgtcgccact ggtaaatgct
    21721 ggcgctattg ccaccaccag cctgattaac gctgaaaata ttgaacaacg ctggcagcga
    21781 attttacata tccaacagca actggctggt gagcaggtag cgctctctga tgaagtcaac
    21841 cagtcggaac aaacaaccaa cttccataac cgggccattg cctggctgct gtactccgcc
    21901 ggatatctct attgtgatgc aatggaagcc tgtgacgtgt acacccgtca gtgctccacg
    21961 ctcatcaata ctgttgaact ggcaacgctt ggcgcgacgc tggcggcggg tggtgtgaat
    22021 ccgctgacgc ataaacgcgt tcttcaggcc gacaacgtgc cgtacattct ggccgaaatg
    22081 atgatggaag ggctgtatgg tcgctccggt gactgggcgt atcgcgttgg tttaccgggc
    22141 aaaagcggtg taggtggcgg tattctggcg gtcgtccctg gcgtgatggg aattgccgcg
    22201 ttctcaccac cgctggacga agaaggcaac agtgttcgcg gtcaaaaaat ggtggcatcg
    22261 gtcgctaagc aactcggcta taacgtgttt aagggctgat catgatgaac acggaaggta
    22321 ataacggtaa caa
//
LOCUS       DAZGLJ010000056        22042 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000056 DAZGLJ010000000
VERSION     DAZGLJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.056"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            158..1882
                     /gene="ilvI"
                     /locus_tag="ACHK49_001966"
     CDS             158..1882
                     /gene="ilvI"
                     /locus_tag="ACHK49_001966"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO9262620.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            1885..2376
                     /gene="ilvN"
                     /locus_tag="ACHK49_001967"
     CDS             1885..2376
                     /gene="ilvN"
                     /locus_tag="ACHK49_001967"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO9262621.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            2388..2474
                     /locus_tag="ACHK49_001968"
     CDS             2388..2474
                     /locus_tag="ACHK49_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO9262622.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            2556..3560
                     /gene="cra"
                     /locus_tag="ACHK49_001969"
     CDS             2556..3560
                     /gene="cra"
                     /locus_tag="ACHK49_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO9262623.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            4162..4620
                     /gene="mraZ"
                     /locus_tag="ACHK49_001970"
     CDS             4162..4620
                     /gene="mraZ"
                     /locus_tag="ACHK49_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO9262624.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            4622..5563
                     /gene="rsmH"
                     /locus_tag="ACHK49_001971"
     CDS             4622..5563
                     /gene="rsmH"
                     /locus_tag="ACHK49_001971"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO9262625.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            5560..5925
                     /gene="ftsL"
                     /locus_tag="ACHK49_001972"
     CDS             5560..5925
                     /gene="ftsL"
                     /locus_tag="ACHK49_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO9262626.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            5941..7707
                     /gene="ftsI"
                     /locus_tag="ACHK49_001973"
     CDS             5941..7707
                     /gene="ftsI"
                     /locus_tag="ACHK49_001973"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO9262627.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            7694..9181
                     /gene="murE"
                     /locus_tag="ACHK49_001974"
     CDS             7694..9181
                     /gene="murE"
                     /locus_tag="ACHK49_001974"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO9262628.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVTAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            9178..10536
                     /gene="murF"
                     /locus_tag="ACHK49_001975"
     CDS             9178..10536
                     /gene="murF"
                     /locus_tag="ACHK49_001975"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO9262629.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            10530..11612
                     /gene="mraY"
                     /locus_tag="ACHK49_001976"
     CDS             10530..11612
                     /gene="mraY"
                     /locus_tag="ACHK49_001976"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO9262630.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGIAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            11615..12931
                     /gene="murD"
                     /locus_tag="ACHK49_001977"
     CDS             11615..12931
                     /gene="murD"
                     /locus_tag="ACHK49_001977"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO9262631.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            12931..14175
                     /gene="ftsW"
                     /locus_tag="ACHK49_001978"
     CDS             12931..14175
                     /gene="ftsW"
                     /locus_tag="ACHK49_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO9262632.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            14172..15239
                     /gene="murG"
                     /locus_tag="ACHK49_001979"
     CDS             14172..15239
                     /gene="murG"
                     /locus_tag="ACHK49_001979"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO9262633.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            15293..16768
                     /gene="murC"
                     /locus_tag="ACHK49_001980"
     CDS             15293..16768
                     /gene="murC"
                     /locus_tag="ACHK49_001980"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO9262634.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISAYNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            16761..17681
                     /gene="ddlB"
                     /locus_tag="ACHK49_001981"
     CDS             16761..17681
                     /gene="ddlB"
                     /locus_tag="ACHK49_001981"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO9262635.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            17683..18513
                     /gene="ftsQ"
                     /locus_tag="ACHK49_001982"
     CDS             17683..18513
                     /gene="ftsQ"
                     /locus_tag="ACHK49_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO9262636.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNHAQAEQQ
                     "
     gene            18510..19772
                     /gene="ftsA"
                     /locus_tag="ACHK49_001983"
     CDS             18510..19772
                     /gene="ftsA"
                     /locus_tag="ACHK49_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO9262637.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            19833..20984
                     /gene="ftsZ"
                     /locus_tag="ACHK49_001984"
     CDS             19833..20984
                     /gene="ftsZ"
                     /locus_tag="ACHK49_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO9262638.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            21085..22002
                     /gene="lpxC"
                     /locus_tag="ACHK49_001985"
     CDS             21085..22002
                     /gene="lpxC"
                     /locus_tag="ACHK49_001985"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO9262639.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
BASE COUNT         5051 a         5327 c         6423 g         5241 t
ORIGIN      
        1 taatccttcg taatggagga ttttatcgtt tcttttcacc tttcctcctg tttattctta
       61 ttaccctgcg tttatgtctc tggctgccaa ttgcttaagc aagatcggac ggttaatgtg
      121 ttttacacat tttttccgtc aaacagtgag gcaggccatg gagatgttgt ctggagccga
      181 gatggtcgtc cgatcgctta tcgatcaggg cgttaaacaa gtattcggtt atcccggagg
      241 cgcagtcctt gatatttacg atgcattgca taccgtgggt ggtatcgatc atgttttagt
      301 tcgtcatgag caggcggcag tgcatatggc cgatggtctg gcgcgcgcga ccggggaagt
      361 cggcgtcgtg ctggtaacgt cgggtccagg ggcgaccaat gcgattaccg gcatcgccac
      421 cgcttatatg gattccattc cattagttgt cctttccggg caggtagcga cctcgttgat
      481 aggttacgat gcctttcagg agtgcgacat ggtggggatt tcgcgaccgg tggttaaaca
      541 cagttttctg gttaagcaaa cggaagacat tccgcaggtg ctgaaaaagg ctttctggct
      601 ggcggcaagt ggtcgcccag gaccagtggt cgttgattta ccgaaagata ttcttaatcc
      661 ggcgaacaaa ttaccctatg tctggcctga gtcggtcagt atgcgttctt acaatcccac
      721 tactaccgga cataaagggc aaattaagcg cgctctgcaa acgctggtag cggcaaaaaa
      781 accggttgtc tacgtaggcg gtggggcaat catggcgggc tgccatcagc agttgaaaga
      841 aacggtggag gcgttgaatc tgcccgttgt ttcctcattg atggggctgg gggcgtttcc
      901 ggcaacgcat cgtcaggcac tgggcatgct gggaatgcac ggtacctacg aagccaatat
      961 gacgatgcat aacgcggatg tgattttcgc cgtcggggta cgatttgatg accgaacgac
     1021 gaacaatctg gcaaagtact gcccaaatgc cactgttctg catatcgata ttgatcctac
     1081 ttccatttct aaaaccgtga ctgcggatat cccgattgtg ggggatgctc gccaggtcct
     1141 cgaacaaatg cttgaactct tgtcgcaaga atccgcccat caaccactgg atgagatccg
     1201 cgactggtgg cagcaaattg aacagtggcg cgctcgtcag tgcctgaaat atgacactca
     1261 cagtgaaaag attaaaccgc aggcggtgat cgagactctt tggcggttga cgaagggaga
     1321 cgcttacgtg acgtccgatg tcgggcagca ccagatgttt gctgcacttt attatccatt
     1381 cgacaaaccg cgtcgctgga tcaattccgg tggcctcggc acgatgggtt ttggtttacc
     1441 tgcggcactg ggcgtcaaaa tggcgttgcc agaagaaacc gtggtttgcg tcactggcga
     1501 cggcagtatt cagatgaaca tccaggaact gtctaccgcg ttgcaatacg agttgccggt
     1561 actggtggtg aatctcaata accgctatct ggggatggtg aaacagtggc aggacatgat
     1621 ctattccggc cgtcattcac aatcttatat gcaatcacta cccgatttcg tccgtctggc
     1681 ggaagcctat ggtcacgtcg ggatccagat ctctcatccg catgagctgg aaagcaaact
     1741 tagcgaggcg ctggaacagg tgtgcaataa tcgcctggtg tttgttgatg ttaccgtcga
     1801 tggcagtgag cacgtctacc cgatgcagat tcgcgggggc ggaatggatg aaatgtggtt
     1861 aagcaaaacg gagagaacct gattatgcgc cggatattat cagtcttact cgaaaatgaa
     1921 tcaggcgcgt tatcccgcgt gattggcctt ttttcccagc gtggctacaa cattgaaagc
     1981 ctgaccgttg cgccaaccga cgatccgaca ttatcgcgta tgaccatcca gaccgtgggc
     2041 gatgaaaaag tgcttgagca gatcgaaaag caattacaca agctggtcga tgtcttgcgc
     2101 gtgagtgagt tggggcaggg cgcgcatgtt gagcgggaaa tcatgctggt gaaaattcag
     2161 gccagcggtt acgggcgtga cgaagtgaaa cgtaatacgg aaatattccg tgggcaaatt
     2221 atcgatgtca ccccctcgct ttataccgtt caattagcag gcaccagcga taagcttgat
     2281 gcatttttag catcgattcg cgatgtggcg aaaattgtgg aagttgctcg ctctggtgtg
     2341 gtcggacttt cgcgcggcga taaaataatg cgttgagaat gatctcagtg cgcaatttgc
     2401 agcccaacat gtcacgttgg gctttttttg cgaaatcagt gggaacctgg aataaaagca
     2461 gttgccgcag ttaattttct gcgcttagat gttaatgaat ttaacccata ccagtacaat
     2521 ggctatggtt tttacatttt acgcaagggg caattgtgaa actggatgaa atcgctcggc
     2581 tggcgggagt gtcgcggacc actgcaagct atgttattaa cggcaaagcg aagcaatacc
     2641 gtgtgagcga caaaaccgtt gaaaaagtca tggctgtggt gcgtgagcac aattaccacc
     2701 cgaacgccgt ggcagctggg cttcgtgctg gacgcacacg ttctattggt cttgtgatcc
     2761 ccgatctgga gaacaccagc tatacccgca tcgctaacta tcttgagcgc caggcgcggc
     2821 aacggggtta tcaactgctg attgcctgct cagaagatca gccagacaac gaaatgcgct
     2881 gcattgagca ccttttacag cgtcaggttg atgccattat tgtttcgacg tcgttgccac
     2941 ctgagcatcc tttttatcaa cgctgggcta acgacccgtt cccgattgtc gcgctggacc
     3001 gcgccctcga tcgtgaacat ttcaccagcg tggttggtgc cgatcaggat gatgccgaaa
     3061 tgctggcgga agagttacgt aagtttcccg ccgagacggt gctttatctt ggcgcgctgc
     3121 cggagctttc tgtcagcttc ctgcgtgaac aaggtttccg tactgcctgg aaagatgatc
     3181 cgcgcgaagt gcatttcctg tatgccaaca gctatgagcg ggaggcggct gcccagttat
     3241 tcgaaaaatg gctggaaacg catccgatgc cgcaggcgct gttcacaacg tcgtttgcgt
     3301 tgttgcaagg ggtgatggat gtcacgctac gtcgcgatgg taaactgcct tctgacctgg
     3361 caattgccac ctttggcgat aacgaactgc tcgacttctt acagtgcccg gtgctggcag
     3421 tggctcaacg tcaccgcgat gtcgcagagc gtgtgctgga gattgtcctg gcaagcctgg
     3481 acgaaccgcg taagccaaaa cctggtttaa cgcgcattaa acgtaatctc tatcgccgcg
     3541 gtgtgctcag ccgtagctaa gccgcgaaca aaaatacgcg ccaggtgaat ttccctctgg
     3601 cgcgtagagt acgggaatgg acatcaatat ccttaaagta aataagacta ttcctgacta
     3661 ttattgataa gttcttttaa acccgcccgt taattaactc accagctgaa attcacaata
     3721 attaagtgat atcgacagcg cgtttttgca ttattttgtt acatgcagag atgaattgcc
     3781 gatttaacaa gcacttttct ttgcttttgc gcaaacccgc tggcatcaag cgccacacag
     3841 acgcaacagg gactgttaac cggggaagat atgtcctaaa atgccgctcg cgtcgcaaac
     3901 tgacacttta tatttgctgt ggaaaatagt gagtcatttt aaaacggtga tgacgatgag
     3961 ggattttttc ttacagctat tcataacgtt aatttgctgc gcacgttgga cgtaaaataa
     4021 acaacgctga tattagccgt aaacatcggg ttttttacct cggtatgcct tgtgactggc
     4081 ttgacaagct tttcctccgc tccgtaaact cctttcagtg ggaaattgtg gggcaaagtg
     4141 ggaataaggg gtgaggctgg catgttccgg ggagcaacgt tagtcaatct cgacagcaaa
     4201 gggcgcttat cagtgcctac ccgttaccgg gaacagctgc ttgagaacgc tgccggtcaa
     4261 atggtttgca ccattgacat tcatcacccg tgcctgctgc tttaccccct acctgaatgg
     4321 gaaattatcg agcaaaaatt atcgcgtctg tcgagcatga acccggttga gcgccgtgtg
     4381 cagcgcctac tgttaggtca tgccagcgaa tgtcagatgg atggcgcagg tcgattgtta
     4441 atcgcgccag tactgcggca acatgccggg ctgacaaaag aagtgatgct ggttggacag
     4501 ttcaacaagt ttgagctgtg ggatgaaaca acctggcatc aacaggtcaa ggaagatatc
     4561 gacgcagagc agttggctac cggagactta tcggagcgac tgcaggactt gtctctataa
     4621 aatgatggaa aactataaac atactacggt gctgctggat gaagccgtta atggcctcaa
     4681 tatccgtcct gatggcatct acattgatgg gacttttggt cgcggtggtc actcacgtct
     4741 gatcctctcg cagcttggcg aagaggggcg tttgctggcg atcgatcgcg acccgcaggc
     4801 tatcgccgtt gcgaagacta ttgatgatcc gcgcttctcc atcatccacg gacctttctc
     4861 cgcgctgggc gaatacgttg ccgagcgcga tcttatcggc aagatcgacg gcattctcct
     4921 cgatcttggc gtctcttcac cgcaacttga tgatgctgag cgcggctttt cctttatgcg
     4981 cgatggtccg ctggacatgc gtatggaccc aacccgtggg cagtcagccg ctgaatggct
     5041 acaaaccgca gaagaagccg atatcgcctg ggtattgaaa acctatggtg aagagcgttt
     5101 tgccaaacgc attgcccgcg ccattgtcga gcgtaaccgc gaacagccga tgacccgcac
     5161 caaagaactg gcggaagtcg tggctgctgc aacgccggtg aaagataagt ttaaacatcc
     5221 cgcgacccgt accttccagg cggtgcgcat ttgggtaaac agtgaactgg aggagataga
     5281 gcaggcgcta aaaagctcgc tcaacgtgct ggccccgggt gggcggcttt cgatcatcag
     5341 cttccactcg ctggaagacc gtattgtgaa acgttttatg cgtgaaaaca gccgcggtcc
     5401 gcaagttccg gcagggttac cgatgactga agagcagctc aaaaaactgg gtggccgtca
     5461 gctgcgagca ctaggcaagt taatgccggg cgaagaagag gtggctgaga accctcgtgc
     5521 ccgtagttca gttctgcgta ttgcagagag gacgaatgca tgatcagcag agtgacagaa
     5581 gctctaagca aagttaaagg atcgatggga agccacgagc gccatgcatt gcctggtgtt
     5641 atcggtgacg atcttttgcg atttgggaag ctgccactct gcctgttcat ttgcattatt
     5701 ttgacggcgg tgactgtggt aaccacggcg caccataccc gtttactgac cgctcagcgc
     5761 gaacaactgg tgctggagcg agatgcttta gacattgaat ggcgcaacct gatccttgaa
     5821 gagaatgcgc tcggcgacca tagccgggtg gaaaggatcg ccacggaaaa gctgcaaatg
     5881 cagcatgttg atccgtcaca agaaaatatc gtagtgcaaa aataaggata aacgcgacgc
     5941 atgaaagcag cggcgaaaac gcagaaacca aaacgtcagg aagaacatgc caactttatc
     6001 agttggcgtt ttgcgttgtt atgcggctgt attctcctgg cgctggcttt tctgctcgga
     6061 cgcgtagcgt ggttacaagt tatctccccg gatatgctgg tgaaagaggg cgacatgcgt
     6121 tctcttcgcg ttcagcaagt ttccacctcc cgcggcatga ttactgaccg ttctggtcgc
     6181 ccgttagcgg tgagcgtgcc ggtaaaagcg atttgggctg acccgaaaga agtgcatgac
     6241 gctggcggta tcagcgtcgg tgaccgctgg aaggcgctgg ctaacgcgct caatattccg
     6301 ctggatcagc tttcagcccg cattaacgcc aacccgaaag ggcgctttat ttatctggcg
     6361 cgtcaggtga accctgacat ggcggactac atcaaaaaac tgaaactgcc ggggattcat
     6421 ctgcgtgaag agtctcgccg ttactatccg tccggcgaag tgactgctca cctcatcggc
     6481 tttactaacg tcgatagtca agggattgag ggcgttgaga agagtttcga taaatggctt
     6541 accgggcagc cgggtgagcg cattgtgcgt aaagaccgct atggtcgcgt aattgaagat
     6601 atttcttcta ctgacagcca ggcagcgcac aacctggcgc tgagtattga tgaacgcctg
     6661 caggcgctgg tttatcgcga actgaacaac gcggtggcct ttaacaaggc tgaatctggt
     6721 agcgccgtgc tggtggatgt caacaccggt gaagtgctgg cgatggctaa cagcccgtca
     6781 tacaacccta acaatctgag cggcacgccg aaagaggcga tgcgtaaccg taccatcacc
     6841 gacgtgtttg aaccgggctc aacggttaaa ccgatggtgg taatgaccgc gttgcaacgt
     6901 ggcgtggtgc gggaaaactc ggtactcaat accattcctt atcgaattaa cggccacgaa
     6961 atcaaagacg tggcacgcta cagcgaatta accctgaccg gggtattaca gaagtcgagt
     7021 aacgtcggtg tttccaagct ggcgttagcg atgccgtcct cagcgttagt agatacttac
     7081 tcacgttttg gactgggaaa agcgaccaat ttggggttgg tcggagaacg cagtggctta
     7141 tatcctcaaa aacaacggtg gtctgacata gagagggcca ccttctcttt cggctacggg
     7201 ctaatggtaa caccgttaca gttagcgcga gtctacgcaa ctatcggcag ctacggcatt
     7261 tatcgcccac tgtcgattac caaagttgac cccccggttc ccggtgaacg tgtcttcccg
     7321 gaatccattg tccgcactgt ggtgcatatg atggaaagcg tggcgctacc aggcggcggc
     7381 ggcgtgaagg cggcgattaa aggctatcgt atcgccatta aaaccggtac cgcgaaaaag
     7441 gtcgggccgg acggtcgcta catcaataaa tatattgctt ataccgcagg cgttgcgcct
     7501 gcgagtcagc cgcgcttcgc gctggttgtt gttatcaacg atccgcaggc gggtaaatac
     7561 tacggcggcg ccgtttcagc gccggtcttt ggtgccatca tgggcggcgt attgcgtacc
     7621 atgaacatcg agccggatgc gctgacaacg ggcgataaaa atgaatttgt gattaatcaa
     7681 ggcgagggga caggtggcag atcgtaattt gcgcgacctt cttgctccgt gggtgccaga
     7741 cgcaccttcg cgagcactgc gagagatgac actcgacagc cgtgtggctg cggcgggcga
     7801 tctctttgta gctgtagtag gtcatcaggc ggacgggcgt cgatatatcc cgcaggcgat
     7861 agcgcaaggt gtgactgcca ttattgcaga ggcgaaaggt gaggcgaccg acggtgaaat
     7921 ccgtgaaatg cacggcgtac cggtcatcta tctcagccag ctcaacgagc gtttatctgc
     7981 actggcgggc cgcttttacc atgaaccctc tgacaattta cgtctcgtag gcgtaacggg
     8041 caccaacggc aaaaccacga ctacccagct gctggcgcag tggagccaac tgcttggcga
     8101 aaccagcgcg gtaatgggca ccgttggtaa cggtctgctg gggaaagtga tcccgacaga
     8161 aaataccacc ggttcggcag tcgatgttca gcatgagctg gcggggctgg tggatcaggg
     8221 cgcgacgttt tgcgcaatgg aagtttcctc ccacgggctg gtacagcacc gtgtggcggc
     8281 attgaaattt gcggcgtcgg tctttaccaa cttaagccgc gatcaccttg attatcatgg
     8341 tgatatggaa cactacgaag ccgcgaaatg gctgctttat tctgaacatc attgtggtca
     8401 ggcgattgtt aacgccgacg atgaagtggg ccgccgctgg ctggcaaaac tgccggacgc
     8461 ggttgcggta tcaatggaag atcatatcaa tccgaactgt cacggacgct ggttgaaagc
     8521 gaccgaagtg aactatcacg acagcggcgc gaccattcgc tttagttcaa gttggggcga
     8581 tggcgaaatt gaaagccatc tgatgggcgc ttttaacgtc agcaacctgc tgctcgcgct
     8641 ggcgacactg ctggcacttg gctacccgct ggctgatctg ttaaaaaccg ccgcgcgtct
     8701 gcaaccggtt tgcggacgta tggaagtgtt cactgcgcca ggcaaaccga cggtggtggt
     8761 ggattacgcg catacgccgg atgcactgga aaaagcctta caggcggcgc gcctgcactg
     8821 tgcgggcaag ctgtggtgcg tctttggctg tggtggcgat cgcgataaag gtaagcgtcc
     8881 actgatgggc gcaattgccg aagagtttgc tgacgtggcg gtggtgacgg acgataaccc
     8941 gcgtaccgaa gaaccgcgtg ccatcatcaa cgatattctg gcgggaatgt tagatgccgg
     9001 acatgccaaa gtgatggaag gccgtgctga agcggtgact tgcgccgtta tgcaggctaa
     9061 agagaatgat gtggtactgg tcgcgggcaa aggccatgag gattaccaga ttgttggcaa
     9121 tcagcgtctg gactactccg atcgcgtcac ggtggcgcgt ctgctggggg tgattgcatg
     9181 attagcgtaa cccttagcca acttaccgat attctcaacg gtgaactgca aggtgcagat
     9241 atcacccttg atgctgtaac cactgatacc cgaaaactga cgccgggctg cctgtttgtt
     9301 gccctgaaag gcgaacgttt tgatgcccac gattttgccg accaggcgaa agctggcggc
     9361 gcaggcgcac tactggttag ccgtccgctg gacatcgacc tgccgcagtt aatcgtcaag
     9421 gatacgcgtc tggcgtttgg tgaactggct gcatgggttc gccagcaagt tccggcgcgc
     9481 gtggttgctc tgacggggtc ctccggcaaa acctccgtta aagagatgac ggcggcgatt
     9541 ttaagccagt gcggcaacac gctttatacg gcaggcaatc tcaacaacga catcggtgta
     9601 ccgatgacgc tgttgcgctt aacgccggaa tacgattacg cagttattga acttggcgcg
     9661 aaccatcagg gcgaaatagc ctggactgtg agtctgactc gcccggaagc tgcgctggtc
     9721 aacaacctgg cagcggcgca tctggaaggt tttggctcgc ttgcgggtgt cgcgaaagcg
     9781 aaaggtgaaa tctttagcgg cctgccggaa aacggtatcg ccattatgaa cgccgacaac
     9841 aacgactggc tgaactggca gagcgtaatt ggctcacgca aagtgtggcg tttctcaccc
     9901 aatgccgcca acagcgattt caccgccacc aatatccatg tgacctcgca cggtacggaa
     9961 tttaccctac aaaccccaac cggtagcgtc gatgttctgc tgccgttgcc ggggcgtcac
    10021 aatattgcga atgcgctggc agccgctgcg ctctccatgt ccgtgggcgc aacgcttgat
    10081 gctatcaaag cggggctggc aaatctgaaa gctgttccag gccgtctgtt ccccatccaa
    10141 ctggcagaaa accagttgct gctcgacgac tcctacaacg ccaatgtcgg ttcaatgacc
    10201 gccgctgttc aggtactggc tgaaatgccg ggctaccgcg tgctggtggt gggcgatatg
    10261 gcggaactgg gcgctgaaag cgaagcctgc catgtacagg tgggcgaggc ggcaaaagct
    10321 gctggtattg accgcgtgtt aagcgtgggt aaacaaagcc atgctatcag caccgccagc
    10381 ggcgttggcg aacattttgc tgataaaact gcgttaatta cgcgtcttaa atcactgatt
    10441 gctgagcaac aggtaattac gattttagtt aagggttcac gtagtgccgc catggaagag
    10501 gtagtacgcg ctttacagga gaatgggaca tgttagtttg gctggccgaa catttggtca
    10561 aatattattc cggctttaac gtcttttcct atctgacgtt tcgcgccatc gtcagcctgc
    10621 tgaccgcgct gttcatctca ttgtggatgg gcccgcgtat gattgctcat ttgcaaaaac
    10681 tttcctttgg tcaggtggtg cgtaacgacg gtcctgaatc acacttcagc aagcgcggta
    10741 cgccgaccat gggcgggatt atgatcctga cggcgattgt gatctccgta ctgctgtggg
    10801 cttacccgtc caatccgtac gtctggtgcg tgttggtggt gctggtaggt tacggtgtta
    10861 ttggctttgt tgatgattat cgcaaagtgg tgcgtaaaga caccaaaggg ttgatcgctc
    10921 gttggaagta tttctggatg tcggtcattg cgctgggtat cgccttcgcc ctgtaccttg
    10981 ccggcaaaga cacgcccgca acgcagctgg tggtcccatt ctttaaagat gtgatgccgc
    11041 agctggggct gttctacatt ctgctggctt acttcgtcat tgtgggtact ggcaacgcgg
    11101 taaacctgac cgatggtctc gacggcctgg caattatgcc gaccgtattt gtcgccggtg
    11161 gttttgcgct ggtggcgtgg gcgaccggca acatgaactt tgccagctat ttacacatac
    11221 cgtatctgcg acacgccggg gaactggtca ttgtctgcac ggcgatagtc ggggcaggac
    11281 tgggcttcct gtggtttaac acctatccgg cgcaggtctt tatgggcgat gtaggttcgc
    11341 tggcgttagg tggtgcgtta ggcattatcg ccgtactgct acgtcaggaa ttcctgctgg
    11401 tgattatggg gggcgtgttc gtggtagaaa cgctttctgt catcctgcag gtcggctcct
    11461 ttaaactgcg cggacaacgt attttccgca tggcaccgat tcatcaccac tatgaactga
    11521 aaggctggcc ggaaccgcgc gtcattgtgc gtttctggat tatttcgctg atgctggttc
    11581 tgattggtct ggcaacgctg aaggtacgtt aatcatggct gattatcagg gtaaaaatgt
    11641 cgtcattatc ggcctgggcc tcactgggct ttcctgcgtg gactttttcc tcgctcgcgg
    11701 tgtgacgccg cgcgttatgg atacgcgtat gacaccgcct ggcctggata aattacccga
    11761 agccgtagaa cgccacacgg gcagtctgaa tgatgaatgg ctgatggcgg cagatctgat
    11821 tgtcgccagt cccggtattg cactggcgca tccatcctta agcgctgccg ctgatgccgg
    11881 aatcgaaatc gttggcgata tcgagctgtt ctgccgcgaa gcacaagcac cgattgtggc
    11941 gattaccggt tctaacggca aaagcacggt caccacgcta gtgggtgaaa tggcgaaagc
    12001 ggcgggggtt aacgttggtg tgggtggcaa tattggcctg ccagcgttga tgctgcttga
    12061 tgctgagtgc gaactgtacg tgctggaact gtcgagcttc cagctggaaa ccacttccag
    12121 cttacaggcg gtggcggcga ccattctgaa cgtgactgaa gatcatatgg accgctatcc
    12181 gtttggtttg cagcaatacc gtgcggcaaa attgcgtatt tacgaaaatg cgaaagtttg
    12241 tgtcgtcaat gctgatgatg ccttaacaat gccgattcgc ggtgctgatg aacgctgcgt
    12301 cagctttggc gtcaacatgg gtgactatca cctgaatcat cagcagggag aaacctggct
    12361 gcgagtgaaa ggcgagaaag tgctgaacgt gaaagagatg aaactttccg ggcagcataa
    12421 ctacaccaat gcgctggcag cactggcact ggcagacgca gctggtttgc cgcgcgccag
    12481 cagcctgaaa gcgttaacca ccttcaccgg tctgccgcat cgctttgaag tggtgctgga
    12541 gcacaatggc gtacgctgga ttaacgattc gaaagccact aacgtcggca gtacggaagc
    12601 ggcgttgaat ggcttgcagg tagacggcac gctgcatttg ttgctgggcg gcgatggtaa
    12661 atcggcggac ttcagtccac tggcgcgcta tctgaatggc gacaacgtgc gtttgtactg
    12721 ttttggtcgt gacggtgcgc agctggcggc gctacgcccg gaagtggcag aacaaaccga
    12781 aactatggaa caggcgatgc gcttgctggc tccgcgtgtt cagccgggcg atatggttct
    12841 gctctccccg gcctgtgcca gccttgatca gttcaagaac tttgaacaac gaggcaatga
    12901 gtttgcccgt ctggcgaagg agttaggttg atgcgtttat ctctccctcg cctgaaaatg
    12961 ccgcgcctgc caggattcag tatcctggtc tggatctcca cggcgctaaa aggctgggtg
    13021 atgggctcgc gggaaaaaga taccgacagc ctgatcatgt acgatcgcac cttactgtgg
    13081 ctgaccttcg gccttgcggc gattggcttt atcatggtga cctcggcgtc aatgcccata
    13141 gggcaacgct taaccaacga tccgttcttc ttcgcgaagc gtgatggcgt ctatctgatc
    13201 ctggcattca ttctggcgat cattacgctg cgtctgccga tggagttctg gcaacgctac
    13261 agtgccacga tgctgctcgg atctatcatc ctgctgatga tcgtgctggt agtgggtagc
    13321 tcggttaaag gggcatcgcg ttggatcgat ctcggtttgc tgcgtatcca gcctgcggag
    13381 ctgacaaaac tgtcgctgtt ttgttatatc gccaactatc tggtgcgtaa aggcgacgaa
    13441 gtacgtaata acctgcgcgg cttcctgaaa ccgatgggcg tgattctggt gttggcagtg
    13501 ttactgctgg cacagccaga ccttggtacg gtggtggtgt tgtttgtgac tacgctggcg
    13561 atgttgttcc tggcgggagc gaaattgtgg cagttcattg ccattatcgg tatgggcatt
    13621 tcagcggttg tgttgctgat actcgccgaa ccgtaccgta tccgccgtgt taccgcattc
    13681 tggaacccgt gggaagatcc ctttggcagc ggctatcagt taacgcaatc gctgatggcg
    13741 tttggtcgcg gcgaactttg gggacaaggt ttaggtaact cggtacaaaa actggagtat
    13801 ctgccggaag cgcacactga ctttattttc gccattatcg gcgaagaact ggggtatgtc
    13861 ggtgtggtgc tggcactttt aatggtattc ttcgtcgctt ttcgcgcgat gtcgattggc
    13921 cgcaaagcgt tagaaattga ccaccgtttt tccggttttc tcgcctgttc tattggcatc
    13981 tggtttagct tccaggcgct ggttaacgta ggcgcggcgg cggggatgtt accgaccaaa
    14041 ggtctgacat tgccgctgat cagttacggt ggttcgagct tactgattat gtcgacagcc
    14101 atcatgatgc tgttgcgtat tgattatgaa acgcgtctgg agaaagcgca ggcgtttgta
    14161 cgaggttcac gatgagtggt caaggaaagc gattaatggt gatggcaggc ggaaccggtg
    14221 gacatgtatt cccgggactg gcggttgcgc accatctaat ggctcagggt tggcaagttc
    14281 gctggctggg gactgccgac cgtatggaag cggacttagt gccaaaacat ggcatcgaaa
    14341 ttgatttcat tcgtatctct ggtctgcgtg gaaaaggtat aaaagcactg atagctgcgc
    14401 cgctgcgtat cttcaacgcc tggcgtcagg cgcgggcgat tatgaaagcg tacaaacctg
    14461 acgtggtact cggtatgggc ggctatgtat caggtccagg tggtctggcc gcgtggtcgt
    14521 taggcattcc ggttgtactt catgaacaaa acggtattgc gggcttaacc aataaatggc
    14581 tggcgaagat tgccaccaaa gtgatgcagg cgtttccagg cgctttccct aatgcggaag
    14641 tggtgggtaa cccggtgcgt accgatgtac tggcgctgcc gttgccgcag caacgtttgg
    14701 ctggacgtga aggtccggtt cgtgtgctgg tagtgggtgg ttcccagggc gcacgcattc
    14761 ttaaccagac aatgccgcag gttgctgcga aactgggtga ttcagtcact atctggcatc
    14821 agagcggtaa aggttcgcaa caatccgttg aacaggcgta tgccgaagcg gggcaaccgc
    14881 agcataaagt gacggaattt attgatgata tggcggcggc gtatgcgtgg gcggatgtcg
    14941 tcgtttgccg ctccggtgct ttaacggtga gtgaaatcgc cgcggcagga ctaccggcgt
    15001 tgtttgtgcc gtttcaacat aaagaccgcc agcaatactg gaatgcgcta ccgctggaaa
    15061 aagcgggcgc agccaaaatt atcgagcagc cacagcttag cgtggatgct gtcgccaaca
    15121 ccctggccgg gtggtcgcga gaaaccttat taaccatggc agaacgcgcc cgcgctgcat
    15181 ccattccgga tgccaccgag cgagtggcaa atgaagtgag ccgggctgcc cgggcgtaat
    15241 tgtagcgatg ccttttgcat cgtatgaatt taagaagtta atggcgtaaa gaatgaatac
    15301 acaacaattg gcaaaactgc gttccatcgt gcccgaaatg cgtcgcgttc ggcacataca
    15361 ttttgtcggc atcggtggtg ccggtatggg cggtattgcc gaagttctgg ccaatgaagg
    15421 ttatcagatc agtggttccg atttagcgcc aaatccggtc acgcagcagt taatgaatct
    15481 gggagcgacg atttatttca accatcgccc ggaaaacgta cgtgatgcca gcgtggtcgt
    15541 tgtttccagc gccatttctg cctataaccc ggaaattgtc gcagctcatg aagcgcgtat
    15601 tccggtgatc cgtcgtgccg aaatgctggc tgagttaatg cgttttcgtc atggcatcgc
    15661 cattgcagga acgcacggca aaacgacaac caccgcgatg gtttccagca tctacgcaga
    15721 agcggggctc gacccaacct tcgttaacgg cgggctggta aaagcggcgg gggttcatgc
    15781 gcgtttgggg catggtcggt acctgattgc cgaagcagat gagagtgatg catcgttcct
    15841 gcatctgcaa ccgatggtgg cgattgtcac caatatcgaa gccgaccaca tggataccta
    15901 ccagggcgac tttgagaatt taaaacagac ttttattaat tttctgcaca acctgccgtt
    15961 ttacggtcgt gcggtgatgt gtgttgatga tccggtgatc cgcgaattgt taccgcgagt
    16021 gggacgtcag accacgactt acggcttcag cgaagatgcc gacgtgcgtg tagaagatta
    16081 tcagcagatt ggcccgcagg ggcactttac gctgctgcgc caggacaaag agccgatgcg
    16141 cgtcaccctg aatgcgccag gtcgtcataa cgcgctgaac gccgcagctg cggttgcggt
    16201 tgctacggaa gagggaattg atgacgaggc tattttacgt gcgctggaga gcttccaggg
    16261 gactgggcgc cgttttgatt tcctcggtga attcccgctg gagccagtga atggtaaaag
    16321 cggtacggca atgctggtcg atgactacgg ccaccacccg acggaagttg acgccaccat
    16381 taaagcggcg cgcgcaggct ggccggataa aaacctggta atgctgtttc agccgcaccg
    16441 ttttacccgt acgcgcgacc tgtatgatga tttcgccaat gtgctgacgc aggttgatac
    16501 cctgttgatg ctggaagtgt atccggctgg cgaagcgcca attccgggag cggacagccg
    16561 ttcgctgtgt cgcacaattc gtggacgtgg gaaaattgat cccattctgg tgccggaccc
    16621 tgcgcaggta gccgagatgc tggcaccggt attaaccggt aacgacctga ttctcgttca
    16681 gggggctggt aatatcggaa aaattgcccg ttctttagct gaaatcaaac tgaagccgca
    16741 aactccggag gaagaacaac atgactgata aaatcgcggt cctgttgggc gggacttccg
    16801 ctgagcggga agtttctctg aattctggcg cagcggtgtt agccgggctg cgtgaaggcg
    16861 gtattgatgc gtatcctgtc gacccgaaag aagtcgacgt gacgcaactg aagtcgatgg
    16921 gttttcagaa agtgtttatc gcgctacacg gtcgcggcgg tgaagatggt acgctgcagg
    16981 gcatgctcga gctgatgggc ttgccttata ccggcagcgg agtgatggca tctgcgcttt
    17041 caatggataa actacgcagc aaactgctgt ggcaaggtgc cggtttaccg gtcgcgccgt
    17101 gggtagcgtt aacccgcgca gagtttgaaa aaggcctgag cgataagcag ttagcagaaa
    17161 tttctgctct gggtttgccg gttatcgtta agccgagccg cgaaggttcc agtgtgggaa
    17221 tgtcaaaagt agtagcagaa aatgctctac aagatgcatt aagattggca tttcagcacg
    17281 atgaagaagt attgattgaa aaatggctaa gtgggccgga gttcacggtt gcgatactcg
    17341 gtgaagaaat tttaccgtca atacgtattc aaccgtccgg aaccttctat gattatgagg
    17401 cgaagtatct ctctgatgag acacagtatt tctgccccgc aggtctggaa gcgtcacaag
    17461 aggccaattt gcaggcatta gtgctgaaag catggacgac gttaggttgc aaaggatggg
    17521 gacgtattga cgttatgctg gacagcgatg gacagtttta tctgctggaa gccaatacct
    17581 caccgggtat gaccagccac agcctggtgc cgatggcggc acgtcaggca ggtatgagct
    17641 tctcgcagtt ggtagtacga attctggaac tggcggacta atatgtcgca ggctgctctg
    17701 aacacgcgaa atagcgaaga agaggtttct tctcgccgca ataatggaac gcgtctggcg
    17761 gggatccttt tcctgctgac cgttttaacg acagtgttgg tgagcggctg ggtcgtgttg
    17821 ggctggatgg aagatgcgca acgcctgccg ctctcaaagc tggtgttgac cggtgaacgc
    17881 cattacacgc gtaatgacga tatccggcag tcgatcctgg cattgggtga gccgggtacc
    17941 tttatgaccc aagatgtcaa catcatccag acgcaaatag aacaacgcct gccgtggatt
    18001 aagcaggtga gcgtcagaaa gcagtggcct gatgaattga agattcatct ggttgaatat
    18061 gtgccgattg cgcggtggaa tgatcaacat atggtagacg cggaaggaaa caccttcagc
    18121 gtgccgccag atcgcaccag caagcaggtg cttccaatgc tgtatggccc ggaaggcagc
    18181 gccaatgaag tgttgcaggg ctatcgcgaa atggggcaga tgctggcaaa ggacagattt
    18241 actctgaagg aagcggcgat gaccgcgcgg cgttcctggc agttgacgct gaataacgat
    18301 attaagctca atcttggccg gggcgatacg atgaaacgtt tggctcgctt tgtagaactt
    18361 tatccggttt tacagcagca ggcgcaaacc gatggcaaac ggattagcta cgttgatttg
    18421 cgttatgact ctggagcggc agtaggctgg gcgcccttgc cgccagagga atctactcag
    18481 caacaaaatc atgcacaggc agaacaacaa tgatcaaggc gacggacaga aaactggtag
    18541 taggactgga gattggtacc gcgaaggttg ccgctttagt aggggaagtt ctgcccgacg
    18601 gtatggtcaa tatcattggc gtgggcagct gcccgtcacg tggtatggat aaaggcgggg
    18661 tgaacgacct cgaatccgtg gtcaagtgcg tacaacgcgc cattgaccag gcagaattga
    18721 tggcagattg tcagatctct tcggtatatc tggcgctttc tggtaagcac atcagctgcc
    18781 agaatgaaat tggtatggtg cctatttctg aagaagaagt gacgcaagaa gatgtggaaa
    18841 acgtcgtcca taccgcgaaa tcggtgcgtg tgcgcgatga gcatcgtgtg ctgcatgtga
    18901 tcccgcaaga gtatgcgatt gactatcagg aagggatcaa gaatccggta ggactttcgg
    18961 gcgtgcggat gcaggcaaaa gtgcacctga tcacatgtca caacgatatg gcgaaaaaca
    19021 tcgtcaaagc ggttgaacgt tgtgggctga aagttgacca actgatattt gccggactgg
    19081 catcaagtta ttcggtattg acggaagatg aacgtgaact gggtgtctgc gtcgtcgata
    19141 tcggtggtgg tacaatggat atcgccgttt ataccggtgg ggcattgcgc cacactaagg
    19201 taattcctta tgctggcaat gtcgtgacca gtgatatcgc ttacgccttt ggcacgccgc
    19261 caagcgacgc cgaagcgatt aaagttcgcc acggttgtgc gctgggttcc atcgttggaa
    19321 aagatgagag cgtggaagtg ccgagcgtag gtggtcgtcc gccgcggagt ctgcaacgtc
    19381 agaccctggc agaggtgatc gagccgcgct ataccgagct gctcaacctg gtcaacgaag
    19441 agatattgca gttgcaggaa aagcttcgcc aacaaggggt taaacatcac ctggcggcag
    19501 gcattgtatt aaccggtggc gcagcgcaga tcgaaggtct tgcagcctgt gctcagcgcg
    19561 tgtttcatac gcaagtgcgt atcggcgcgc cgctgaacat taccggttta acggattatg
    19621 ctcaggagcc gtattattcg acggcggtgg gattgcttca ctatgggaaa gagtcacatc
    19681 ttaacggtga agctgaagta gaaaaacgtg ttacagcatc agttggctcg tggatcaagc
    19741 gactcaatag ttggctgcga aaagagtttt aatttttatg aggccgacga tgattacggc
    19801 ctcaggcgac aggcacaaat cggagagaaa ctatgtttga accaatggaa cttaccaatg
    19861 acgcggtgat taaagtcatc ggcgtcggcg gcggcggcgg taatgctgtt gaacacatgg
    19921 tgcgcgagcg cattgaaggt gttgaattct tcgcggtaaa taccgatgca caagcgctgc
    19981 gtaaaacagc ggttggacag acgattcaaa tcggtagcgg tatcaccaaa ggactgggcg
    20041 ctggcgctaa tccagaagtt ggccgcaatg cggctgatga ggatcgcgat gcattgcgtg
    20101 cggcgctgga aggtgcagac atggtcttta ttgctgcggg tatgggtggt ggtaccggta
    20161 caggtgcagc accagtcgtc gctgaagtgg caaaagattt gggtatcctg accgttgctg
    20221 tcgtcactaa gcctttcaac tttgaaggca agaagcgtat ggcattcgcg gagcagggga
    20281 tcactgaact gtccaagcat gtggactctc tgatcactat cccgaacgac aaactgctga
    20341 aagttctggg ccgcggtatc tccctgctgg atgcgtttgg cgcagcgaac gatgtactga
    20401 aaggcgctgt gcaaggtatc gctgaactga ttactcgtcc gggtttgatg aacgtggact
    20461 ttgcagacgt acgcaccgta atgtctgaga tgggctacgc aatgatgggt tctggcgtgg
    20521 cgagcggtga agaccgtgcg gaagaagctg ctgaaatggc tatctcttct ccgctgctgg
    20581 aagatatcga cctgtctggc gcgcgcggcg tgctggttaa catcacggcg ggcttcgacc
    20641 tgcgtctgga tgagttcgaa acggtaggta acaccatccg tgcatttgct tccgacaacg
    20701 cgactgtggt tatcggtact tctcttgacc cggatatgaa tgacgagctg cgcgtaaccg
    20761 ttgttgcgac aggtatcggc atggacaaac gtcctgaaat cactctggtg accaataagc
    20821 aggttcagca gccagtgatg gatcgctacc agcagcatgg gatggctccg ctgacccagg
    20881 agcagaagcc ggttgctaaa gtcgtgaatg acaatgcgcc gcaaactgcg aaagagccgg
    20941 attatctgga tatcccagca ttcctgcgta agcaagctga ttaagaattg actggaattt
    21001 gggtttcgag gctctttgtg ctaaactggc ccgccgaatg tatagtacac ttcggttgga
    21061 taggtaattt ggcgagataa tacgatgatc aaacaaagga cacttaaacg tatcgttcag
    21121 gcgacgggtg tcggtttaca taccggcaag aaagtcaccc tgacgttacg ccctgcgccg
    21181 gccaacaccg gggtcatcta tcgtcgcacc gacttgaatc caccggtaga tttcccggcc
    21241 gatgccaaat ctgtgcgtga taccatgctc tgtacgtgtc tggtcaacga gcatgatgta
    21301 cggatttcaa ccgtagagca cctcaatgct gctctcgcgg gcttgggcat cgataacatt
    21361 gttatcgaag ttaacgcgcc ggaaatcccg atcatggacg gcagcgccgc tccgtttgta
    21421 tacctgctgc ttgacgccgg tatcgacgag ttgaactgcg ccaaaaaatt tgttcgcatc
    21481 aaagagactg ttcgtgtcga agatggcgat aagtgggctg aatttaagcc gtacaatggt
    21541 ttttcgctgg atttcaccat cgattttaac catccggcta ttgattccag caaccagcgc
    21601 tatgcgatga acttctccgc tgatgcgttt atgcgccaga tcagccgtgc gcgtacgttc
    21661 ggtttcatgc gtgatatcga atatctgcag tcccgtggtt tgtgcctggg cggcagcttc
    21721 gattgtgcca tcgttgttga cgattatcgc gtactgaacg aagacggcct gcgttttgaa
    21781 gacgaatttg tgcgtcacaa aatgctcgat gcgatcggtg acttgttcat gtgtggtcac
    21841 aatattattg gtgcatttac cgcttataaa tccggtcatg cactgaataa caaactgctg
    21901 caggctgtcc tggcgaaaca ggaagcctgg gaatatgtga ccttccagga cgacgcagaa
    21961 ctgccgttgg ccttcaaagc gccttcagct gtactggcat aacgacattt atactgtcgt
    22021 ataaaattcg actggcaaat ct
//
LOCUS       DAZGLJ010000057        21847 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000057 DAZGLJ010000000
VERSION     DAZGLJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.057"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(69..977)
                     /gene="melR"
                     /locus_tag="ACHK49_001986"
     CDS             complement(69..977)
                     /gene="melR"
                     /locus_tag="ACHK49_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO9262640.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCKSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRAMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            1260..2615
                     /gene="melA"
                     /locus_tag="ACHK49_001987"
     CDS             1260..2615
                     /gene="melA"
                     /locus_tag="ACHK49_001987"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO9262641.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALHDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2718..4139
                     /gene="melB"
                     /locus_tag="ACHK49_001988"
     CDS             2718..4139
                     /gene="melB"
                     /locus_tag="ACHK49_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO9262642.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(4278..4907)
                     /gene="yjdF"
                     /locus_tag="ACHK49_001989"
     CDS             complement(4278..4907)
                     /gene="yjdF"
                     /locus_tag="ACHK49_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO9262643.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(5030..6676)
                     /gene="fumB"
                     /locus_tag="ACHK49_001990"
     CDS             complement(5030..6676)
                     /gene="fumB"
                     /locus_tag="ACHK49_001990"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO9262644.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(6754..8094)
                     /gene="dcuB"
                     /locus_tag="ACHK49_001991"
     CDS             complement(6754..8094)
                     /gene="dcuB"
                     /locus_tag="ACHK49_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO9262645.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(8665..9384)
                     /gene="dcuR"
                     /locus_tag="ACHK49_001992"
     CDS             complement(8665..9384)
                     /gene="dcuR"
                     /locus_tag="ACHK49_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO9262646.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(9381..11012)
                     /gene="dcuS"
                     /locus_tag="ACHK49_001993"
     CDS             complement(9381..11012)
                     /gene="dcuS"
                     /locus_tag="ACHK49_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO9262647.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            11193..11423
                     /gene="yjdI"
                     /locus_tag="ACHK49_001994"
     CDS             11193..11423
                     /gene="yjdI"
                     /locus_tag="ACHK49_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO9262648.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            11435..11707
                     /gene="yjdJ"
                     /locus_tag="ACHK49_001995"
     CDS             11435..11707
                     /gene="yjdJ"
                     /locus_tag="ACHK49_001995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9262649.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            11934..12230
                     /gene="ghoS"
                     /locus_tag="ACHK49_001996"
     CDS             11934..12230
                     /gene="ghoS"
                     /locus_tag="ACHK49_001996"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO9262650.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            12258..12431
                     /gene="ghoT"
                     /locus_tag="ACHK49_001997"
     CDS             12258..12431
                     /gene="ghoT"
                     /locus_tag="ACHK49_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO9262651.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(12550..14067)
                     /gene="lysS"
                     /locus_tag="ACHK49_001998"
     CDS             complement(12550..14067)
                     /gene="lysS"
                     /locus_tag="ACHK49_001998"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO9262652.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(14304..15761)
                     /gene="dtpC"
                     /locus_tag="ACHK49_001999"
     CDS             complement(14304..15761)
                     /gene="dtpC"
                     /locus_tag="ACHK49_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO9262653.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMAVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(15820..17967)
                     /gene="cadA"
                     /locus_tag="ACHK49_002000"
     CDS             complement(15820..17967)
                     /gene="cadA"
                     /locus_tag="ACHK49_002000"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO9262654.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(18047..19381)
                     /gene="cadB"
                     /locus_tag="ACHK49_002001"
     CDS             complement(18047..19381)
                     /gene="cadB"
                     /locus_tag="ACHK49_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO9262655.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(19747..21285)
                     /gene="cadC"
                     /locus_tag="ACHK49_002002"
     CDS             complement(19747..21285)
                     /gene="cadC"
                     /locus_tag="ACHK49_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO9262656.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(21545..21659)
                     /locus_tag="ACHK49_002003"
                     /pseudo
     CDS             complement(21545..21659)
                     /locus_tag="ACHK49_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         5682 a         5133 c         5325 g         5707 t
ORIGIN      
        1 ttttaaatta ttttcactgc ttattatcga cggctaaact atttttttgg ctgatactga
       61 tatcgtcttt agccgggaaa cgtctggcgg cgctgttggc taagtttgcg gtattgttgc
      121 ggcgacatgc cgacatattt gccgaacgtg ctgtaaaaac gactacttga acgaaagcct
      181 gccgtcaggg caatatcgag aatactttta tcggtatcgc tcagtaacgc gcgaacgtgg
      241 ttgatgcgca tcgcggtaat gtactgtttc atcgtcaatt gcatggcccg ctggaatatc
      301 cccattgcat agttggcgtt aagtttgacg tgctcagcca catcgttgat ggtcagcgcc
      361 tgatcatagt tttcggcaat aaagcccagc atctggctaa cataaaattg cgcatggcgc
      421 gagacgctgt ttttgtgtgt gcgcgaggtt ttattgacca gaatcggttc ccagccagag
      481 aggctaaatc gcttgagcat caggccaatt tcatcaatgg cgagctggcg aatttgctcg
      541 ttcggactgt ttaattcctg ctgccagcgg cgcacttcaa acgggctaag ttgctgtgtg
      601 gccagtgatt tgatcaccat gccgtgagtg acgtggttaa tcaggtcttt atccagcggc
      661 caggagagaa acagatgcat cggcagatta aaaatcgcca tgctcttaca ggttccggta
      721 tctgttagtt ggtgcggtgt acaggcccag aacagcgtga tatgaccctg attgatattc
      781 actttttcat tgttgatcag gtattccaca tcgccatcga aaggcacatt cacttcgacc
      841 tgaccatgcc agtggctggt gggcatgata tgcggtgcgc gaaactcaat ctccatccgc
      901 tggtattccg aatacagcga cagcgggctg cgggtctgtt tttcgtcgct gctgcacata
      961 aacgtatctg tattcatgga tggctctctt tcctggaata tcagaattat ggcaggagtg
     1021 agggaggatg actgcgagtg ggagcacggt tttcaccctc ttcccagaga ggcgagggga
     1081 gcgtccgagc atcatgaggc cgaaaactct gcttttcagg taacttatta ccataaactc
     1141 agatttactg ctgcttcacg caggatctga gtttatggga atgctcaacc tggaagccgg
     1201 aggttttctg cagattcgcc tgccatgatg aagttattca agcaagccag gagatctgca
     1261 tgatgactgc acccaaaatt acatttatcg gcgctggttc gacgattttc gttaaaaata
     1321 ttcttggtga tgtgttccat cgcgaggcgc tgaaaacggc gcatattgcc ctgatggaca
     1381 ttgatcccac ccgcctggaa gagtcgcata ttgtggtgcg taagcttatg gattcagcag
     1441 gggccagcgg caaaatcacc tgccacaccc aacagaaaga agccttacat gatgccgatt
     1501 ttgtcgtggt ggcatttcag attggcggtt atgaaccttg cacggtgact gatttcgagg
     1561 tctgtaagcg gcatggtctg gaacaaacca ttgccgatac gttggggccg ggcggtatta
     1621 tgcgcgcgct acgtaccatt ccgcatctgt ggcaaatttg cgaggacatg acggaagtct
     1681 gccccgatgc caccatgctc aactatgtta acccaatggc gatgaatacc tgggcgatgt
     1741 atgcccgcta tccgcatatc aaacaggtcg ggctgtgcca ttcggtgcag ggaacggcgg
     1801 aagagctggc gcgtgacctc aatatcgacc cagctacgct gcgttaccgt tgcgcaggta
     1861 tcaaccatat ggcgttttac ctggagctgg agcgcaaaac cgccgacggc agttatgtga
     1921 atctctaccc ggaactgctg gcggcttatg aagcagggca ggcaccgaag ccgaatattc
     1981 atggcaatac tcgctgccag aatattgtgc gctacgaaat gttcaaaaag ctgggctact
     2041 tcgtcacgga atcgtcagaa cattttgctg agtacacacc gtggtttatt aagccaggtc
     2101 gtgaggattt gattgagcgt tataaagtac cgctggatga gtacccgaaa cgctgcgtcg
     2161 agcagctggc gaactggcat aaagagctgg aggagtataa aaacgcctcc cggattgata
     2221 ttaaaccgtc acgggaatat gccagcacaa tcatgaacgc tatctggact ggcgagccga
     2281 gtgtgattta cggcaacgtc cgtaacgatg gtttgattga taacctgcca caaggatgtt
     2341 gcgtggaagt agcctgtctg gttgatgcta atggcattca gccgaccaaa gtcggtacgc
     2401 taccttcgca tctggccgcc ctgatgcaaa ccaacatcaa cgtacagacg ctgctgaccg
     2461 aagctattct tacggaaaat cgcgaccgtg tttaccacgc cgcgatgatg gacccgcata
     2521 ctgctgccgt gctgggcatt gatgaaatat atgctcttgt tgacgacctg attgccgccc
     2581 acggcgactg gctgccaggc tggttgcacc gttaaaacgc gactaaacgc tactgcgccg
     2641 ggggatttat tccggcgcac acctctgacg ataccaataa cagaaggcgg gcgttggtaa
     2701 cagcgacccg ataccctatg agcatttcaa tgactacaaa actcagttat ggatttggag
     2761 cgttcgggaa ggattttgcg atcggcattg tgtatatgta cctcatgtat tactacaccg
     2821 atgtcgttgg gctgtctgtg ggtttggtcg gtactttgtt tctggtggcg aggatctggg
     2881 atgctattaa cgatccgatt atgggatgga ttgtaaatgc tacgcgatcg cgatggggta
     2941 agttcaaacc ctggatcctg atcggtacgt tggcaaactc tgtaatctta tttctcctct
     3001 ttagtgcgca tctgtttgaa ggtactactc agattgtctt tgtttgcgtg acctacatcc
     3061 tctggggcat gacttacacc attatggata ttcccttctg gtcgctggtt ccaaccatca
     3121 cgctcgataa acgtgagcgc gaacaactgg ttccttatcc gcgttttttt gccagtctgg
     3181 caggctttgt tacggcaggt gtgacgctac catttgttaa ttatgtcggc ggtggcgatc
     3241 ggggatttgg ctttcagatg ttcactctgg tactaatcgc cttttttatt gtttcaacca
     3301 tcatcactct gcgcaatgtg catgaagtct tttcgtcaga caatcaaccg tctgctgaag
     3361 gaagccatct gacacttaaa gccatcgttg cgctaattta taaaaacgat cagctttcat
     3421 gcctcttggg tatggctctt gcttataatg tagccagcaa cattattacc ggatttgcta
     3481 tctattattt ctcatatgtt atcggtgatg cggatttgtt cccctattat ctgtcgtatg
     3541 cgggagctgc taacctggtg acgttagtat tcttcccacg cttagttaaa tcattatccc
     3601 gacgcatttt atgggccgga gcatctattc ttccggtgtt aagctgtggt gttctcctgt
     3661 taatggcatt aatgagctat cacaacgtcg tcctcattgt gattgcgggt attttgctga
     3721 atgtgggaac ggcgcttttc tgggtattac aggtcatcat ggtggcagat accgttgatt
     3781 acggtgaata taaactgcac gtacgctgtg aaagtatcgc ttactccgtg cagactatgg
     3841 tggtgaaggg cggttcagcc tttgcggctt ttttcattgc ggttgtgtta gggatgattg
     3901 gctatgtacc gaatgttgaa cagtctacgc aagccctatt aggtatgcag tttattatga
     3961 ttgctctacc aactctgttt ttcatggtaa cgctgattct ctacttccgt ttctatcgcc
     4021 tcaatggcga cacgctgcgc aggatccaga ttcatctgct ggataaatat cgcaaagtac
     4081 cgcccgagcc tgttcatgct gatattccgg tcggtgcagt gagtgatgtg aaagcctgac
     4141 gtggacaata gctaacgaaa atggcctgat gcgatatgtt tatcaggcca cgtcttaatg
     4201 taatgctttg gatttgcaaa ttttgtagac cggataagga attcacgccg catccggcat
     4261 caacaaagcg cacgttgtta tccggttatc aagccaaagc gccgtagctg gcggcaatga
     4321 aagcgagcga ggaatatcac cgtcgttaat gcgccaagca gcgcgcagaa catatcggat
     4381 tgcgtgtccc actggtcgcc ctgggtgccg agaaaatcat ccgctccctg gcccattgcc
     4441 agcgccgccc accactcgat taattcatac atcgcgctta tcgccagcgc cacgcagcag
     4501 accaggaacg ccaccatttt acgtccgcgc acgtacatcc cgcgcacgag aatttctcgt
     4561 gccaccagtg caggcaccag cccctggaaa aaatgcccca gcttgtcata cggattacgg
     4621 ctcaacccca gccattcctg cacctcaaaa ccgatgggga ctttcgcgta ggtgtattgc
     4681 ccgccgacca tcaggatgat ggcgtgaaag aaaatgagcg tatagagcag cggcgttaac
     4741 ggataacgtt tagcggtggc aagcagtagc ggcacgacaa taatcaccgg tgtcacttcc
     4801 atcagccagg tgagtttgtc atgggccgaa atgccggtat aaatcaggat taacgttagc
     4861 gccagtgcgc cggtgttaag aattaacggc ttgagtgtac gggtcatggt gagttcgcaa
     4921 gtcagggaaa atactgacta ttcaccagca agcgtgaaat tacaatcgca aaagaggtgg
     4981 cccaggggga tcacctggca gcatgctgcc aggcgctggg ccgaagaggt tacttagtgc
     5041 agttcgcgca ctgtttgttg acgatttgct ggaagaagtc gttacctttg tcatcgacca
     5101 ggataaacgc cgggaaatct tctacttcga ttttccagat agcttccata cccagctccg
     5161 gataagcgac gcactccaga tgcttgatgc tctgctgcgc cagtaccgcc gccggaccgc
     5221 cgatgctacc gaggtagaag ccgccgtgtt tatgacacgc gtcggtaacc tgctgactgc
     5281 ggttaccttt cgccagcatg atcatgctgc cgccgtggga ttgcagcaga tccacgtagg
     5341 agtccatacg gcctgcggtg gttgggccaa gtgaacctga tggataaccg gcaggggttt
     5401 tcgccggacc cgcgtagtag atcgggtgat ctttgatgta ctgcggaagt tctttaccgg
     5461 cgtcaatcag ctctttcagc ttggcgtgtg caatatctcg gcccacgata atggtgccgg
     5521 tgagcgacaa acgagtggat accgggtatt gcgaaagctg ggcgaggatc tctttcatcg
     5581 ggcggttaag gtcaactttc accgcttcgc cttcaccggc ctggcgcagt tcttgtggaa
     5641 tgtactggcc tgggttgtgt tccagttttt cgatccagat accttcgcgg ttgattttcg
     5701 ctttaatgtt acggtcagcg gagcaggaga cgcccatgcc gaccgggcag gatgcgccgt
     5761 gacgtggcag acggataacg cgaatgtcgt gcgcgaagta tttaccgcca aactgcgcgc
     5821 caagaccgag tttctgggcc tcttccagca gttcctgttc cagctggaca tcgcggaacg
     5881 cctgaccatg ttcgttccct tccgtcggca gttcatcgta atagtgagcg cttgctaact
     5941 tgacggtttt caggttggtt tccgcagacg taccgccaat cacaaacgcg atatggtacg
     6001 gcgggcaggc tgcagtaccg agggtacgca ttttctcgac gaggaagttt ttcagtttgc
     6061 cgggagtcag cagggctttg gtttcctggt agagatacgt tttgttggca gagccgccgc
     6121 ctttcgcaac gcaaaggaat ttgtactcat cgccatctac cgcgtacagg tcgatttgcg
     6181 caggcaggtt agtgccggtg ttgacctctt tgtacatgtc cagcgccgca ttctgtgaat
     6241 agcgcaggtt atcttcgata taggtgttat agacgccttt cgacagcgct tcttcatcac
     6301 cgccaccggt ccacacgcgc tggccttttt taccgacgat gatcgcggtg ccggtatcct
     6361 ggcaggtcgg cagcacgcct ttggcggcga tttcggagtt tcttaagaat tgcagcgcca
     6421 cgtacttgtc gttttcgctg gcttctggat cgtgaagaat agccgcaacc tgtttctggt
     6481 gtgccggacg gagcatgaaa gaagcgtcgt gaaaggcttg ctgcgccagt agggtcaggg
     6541 cttctggttc cactttcagg atggtttcgc cgtcgaagtc ggcaacgcta acgtaatcgg
     6601 aagtgagtag atagtattcg gtattgtctt tccccatcgg gaaaggtgcc tggtagataa
     6661 agggtttgtt tgacatagct tccagcctgt aactctgtat ttgttattcg aaaatggcgt
     6721 gccgcgtagt cacgggcggc acgcaaagtg catttataag aacccgtaca tcgcggcgaa
     6781 gatccagccg aagacgcacg atacgctcac accaatcaac cccggcagaa taaaactgtg
     6841 gttgatgacg aagcgaccga tgtgggtggt gccggaacgg tcaaactgaa tcgctgccag
     6901 atcgctcgga taagtcggca ggatgtaata accgtagcaa gccggtgctg aagccacgat
     6961 gtatgccgga tcaacgccga tcgccagcgc gaccggaaca atcgccgcca gcgccgcagc
     7021 ctgagagttt acaaacttgg aaaccagcag cagaacaatg gcatacgccc acggatactc
     7081 tttcaccatt tcacccagta cgccctgaat ttcagacata tgcgcaccga acatggtttc
     7141 tgccatccat gcgataccgt acaccgccac gatggcgatc ataccggaac ggaagacttc
     7201 gttttttgag atagacgcgg gattggtttt ggtcaggata ataatcagcg ccccggtcag
     7261 cagcataaac atctgaataa ccagtaccat cgacagcggt ttgccgccga aggatggacg
     7321 caggtccgaa tcagcaccaa gaagggcgac tacagcgatt gcaccgagga aaatccacat
     7381 tgccagccag ttgcttttcg gcagtttttt atcgagcagc gtcgcggtat caccgtaaac
     7441 atactcacgg ttttccggta cggagatgaa tttctggaac tcttcgtctt tatccagatc
     7501 tttaccgcgg aaccagctga agataccgat cgccaggata ccgattaacg tcgatggaat
     7561 ggtgattgcc agcaggtcga ggaactcaag atggcgacca tcaaaggtga cattacccag
     7621 cattgcaacc agagacacga ccgcaaccga caccggactg gcgataatcc ccatctgtgc
     7681 accgatagaa cttgccgcca tcggacgttc cggacggatg ttgttcttaa tggcgacgtc
     7741 gtagatgatc ggcagaatgg tgtaaaccac atgacccgta ccgcaaagaa tggtcagtgt
     7801 acaggtcaca aacggcgcga caattgagac atatttcggg ttgcggcgca gcagcttctc
     7861 ggcaatttgc agcatgacat caagaccgcc cgaagcttgc aaggtcgccg atgccgccac
     7921 caccgcaatg ataaccagca tgacatcaac cggtggttta cctggctgaa ggtggaagac
     7981 gaagaccaga atgaccagac cgataccgcc taataaaccc agcgcgatac caccctttct
     8041 ggcaccataa aacagacata tcagtattat gataagttgg atagtaaata acatgtgtga
     8101 accctcgcga taatcctatt taaatttttg ctgaatagat cacagtcacg ttctgttttg
     8161 tatgaactgt tttcagagcc tgactaaata tccgtttggt cgttaccggc tttagcaaat
     8221 acctcacagt aaatattggc tgattaaagc agcggcaaat ttatgcactg tgataagcgg
     8281 ctttttcaga gagaaaaact ctctggcgat gggtttatag tttgtactgc gcagatgtta
     8341 atgttttgtt tgctgttatc ttattgatat ttatcggtct attttttgtt ctcagggcag
     8401 atatcattag gtttaaagat taatctgatc tacccatttg tgggtaaaaa tacacataat
     8461 gcgggtgaca taatagttaa ttaacttttg ttatcgtttt gaaattaaaa acaccgttca
     8521 cctgaagaga tattaatttt tagcgatgat ggagggataa ttatatttga tctggcacaa
     8581 gttttactga tgaaggatgt aacttgtgcc aggggtattt tgcattacgg taataattat
     8641 tacttacaga taaccagcga tccgttattg gcaatattgt ttcagtagtg agtagtgttc
     8701 tgcctgaata cggtaacggt aaaccggacg ccccgtgacg ccataatgga tactggtgaa
     8761 caagatgtgg cagttgacca gccagatgag gtatttacgg caggaaacac gcgagatgtt
     8821 aacctcgtta gccagctcgt cggttgaaaa ctcatagtcc tgatgcgcgt caatccactg
     8881 gcacagcgtg cgtaaagtct gcggcgttaa gccttttggc aagcgacgag gatcctgttc
     8941 gttggagctg ctgccgtgga ttagctgatc aagctcggcc tggtcataat actgatgttt
     9001 ttccagcgcc attttctttt gccgccagcc ggtgagcgcc tcttcaaagc gggaagcctg
     9061 gaagggtttg atcaggtaat ccacgacacc gtaatgcagc gaatctttaa tggttgccgc
     9121 atcggctgcg gaggagatga caatcacatc acttttgcaa cgcgcgttat gcaggacagg
     9181 cagtaaatcg agcccgttct ctttttgcat atagatatcg agcaatatca ggtcgatagg
     9241 cgtatcgcta ttgaagataa tctctttggc tttctccagc gtcgaggctg ttccacagca
     9301 ttgaaagcct gggatttgtg ctacgtatcg gcgattcagc tccgcgacca ttgcgtcgtc
     9361 atcgataatt aatacattga tcatctgttc gacctctccc cgtcccaggg tatctggaca
     9421 aaaaattgtg tgaaaatccc gggttccgat tccacggcga tgctgccgcc gagattttct
     9481 acctgttgtt tgacaagtgc taaaccgacg cctcgctcgc ttccttttgt cgagacacct
     9541 ttgtcaaaaa tgtgatcgat tttgtcgggt gcgattcccg gtccatcatc gttaacttcg
     9601 cagtgcagcc agccgtgacg gtagtgcaat gttacgctaa tttcgccacc gggttccggc
     9661 cctaatgctt ccagcgcgtt ttctatcaga tttcccaatg tggtaatcag cgtcgcgacc
     9721 tggtcctcac tgccgctgtc tggcagctgg ctttcactgt ttaaaatcag cgtatggcct
     9781 aaatcggtcg cgcggttaat cttgctgatt aaaaaaccag cgataaccgg agatttgatc
     9841 ttacccagca gagagccaat ctcttcctga tagttattgg ctgttttgag aatgtaatct
     9901 tccaactgct tataactctt cagatgcaat aatccgagaa tcacatgcaa tttattcata
     9961 aattcgtggg atcgttcacg aagtgcatca gcatagttga ccagaccgtc gagtcgctgc
    10021 atcagtttac gtacttcagt tttgtccctg aaggttgaaa tggcaccgat gataacgcca
    10081 ttactgcgca ccggaacggt gttgatcagt aatagccggt ctttaatcgt aatctcttcg
    10141 tcgcggcgcg gcgtaccgtc gcgtaacact tccgagacat ctaccacctg tgaccatgag
    10201 tggctgagcg tcgacagttt ctcatcgtcc tgcgacttac ggtaattcag caattcttgt
    10261 gcggcatcgt tgatcagcgt gacctcgccg cgatcgtcca cggcaacgac gccttctttg
    10321 atagactgca acatggcctg gcgctgctca aacagcgtgg agatttcgta gggttccagg
    10381 ccgaaaagga tttttttcag taccttaacc agaatgcagg tgccaatcag tccgaccagc
    10441 atgccaaata ataccgacca gataatgctc cagcgactgt cattgatttg ttgggtcacg
    10501 cggcttaact caaggccgat cgccaccacg ccaatttgtt tatgattttc atcgtagatg
    10561 ggggtaaata cgcgtaaagc ctgcgccaga aaaccgcgat tgatagcgac attttctttg
    10621 ccattcagcg ctttaaggat gtcatcacct ttaaatggct gaccaatacg ctgggcttca
    10681 ggatgcgagt agcgaagact ttgcatatcg gtaacgacaa taaacagcag atcgttgcgt
    10741 ttgcgcacgg cttccgcgat ggcctggatt ccgctctcct gcggtttttt ctgcaagccc
    10801 tgacggattt ccggcgagtc ggcgagggta cgcgccactg ccagtgcctt gttggccagc
    10861 ccgtctcgcg tcatatcact gatttgcgag aagtaaatca gatgcaccac caatagcacc
    10921 gagaacagta ccgcgctgac cattaagatc actgtggtac tcaatttcat cggacgtttg
    10981 cgtaacatgc ggtagggcaa tgaatgtctc atcagcttcc ttgtgtgaca aatttcttaa
    11041 gcattatctc tgatgaggcg ggtaattcaa agggagtaag aatgattggc tatatagggg
    11101 aagagactct ggcaacggaa actgccagtg ctgtatgaag attccagggc tatgcttata
    11161 gcgataatca tactgatgag agagggaaag tcatggatca ggcgctactg gacgagggtt
    11221 atcgctgtta taccggcgaa aagatcgatg tctatttcaa cactgcgata tgtcagcatt
    11281 ctggcaattg cgtacgtggc aacggcaagt tatttaatct caaacgaaag ccgtggatca
    11341 tgccggatga agtcgacgtc gtcactgtgg ttaaagtgat tgatacgtgc ccgagcggcg
    11401 cgctgaaata ccgtcataaa taagcgaggg taaaatggaa atacgcgaag gccacaataa
    11461 attttacatt aatgacgaac aaggcaagca aatcgctgaa attgtctttg tgccgaccgg
    11521 agagaattta gcgattatcg aacataccga tgtcgatgaa agcctgaaag ggcaggggat
    11581 tggtaaacag ctggttgcga aagtcgtgga aaaaatgcgt cgggaaaaac gaaaaattat
    11641 cccactatgc ccatttgcga aacacgaatt tgataaaacg cgggagtatg atgatattcg
    11701 cagttgatgg gagagtacag agtcacgata tttttcatgc tctccgcgga gtgatgcagg
    11761 agagcatctg aagggtaggg ggatgcacaa agaatgggca gagagcgcgt ttttttgtcc
    11821 caagtcatcc cctttactga gcaaaaaaaa gaatatctcc tatatgagaa tcatcaatcg
    11881 gggttaataa tttttgcgtc cccagagcat tcaatattga taggagtcat attatggaag
    11941 gtaaaaacaa gttcaatact tatgttgttt cttttgatta tccatcatct tattcctcag
    12001 tgttcttaag gttaagatca ttgatgtatg atatgaattt ctcttctatc gtggctgatg
    12061 aatatgggat accacgacaa ttgaatgaaa attccttcgc aataacgaca tcgttagccg
    12121 caagtgaaat cgaagattta atcaggctta aatgcttaga cttaccggat attgattttg
    12181 acctcaacat tatgacagtt gatgactatt tccgtcagtt ttacaagtag ccaggaagga
    12241 taagaagaac ataaaaaatg gcactattct ctaaaatatt aattttttat gtgattggtg
    12301 tgaacatatc ctttgtcatt atctggttta tctcacatga gaaaacacat attcgtttac
    12361 ttagtgcatt cctggtcgga ataacctggc caatgagtct gcctgtggca ttactttttt
    12421 ctctctttta ggagctgtag ttgagtggac ccgctggtta tcgtgaaacg aagcgtctct
    12481 cctcaaaact ggatttgcgg aacgacgaac gaagcagtcg cctgactgct tcgttcatta
    12541 aagtgaaatt tatttctgtg ggcgcatcgc cgggaagaga ataacgtcgc gaatagtatg
    12601 actgtttgta aacagcataa tcattcggtc gataccaata cccagaccag cggttggcgg
    12661 cagaccatat tccagcgcag tcacgtaatc ttcgtcatag aacatggctt cgtcgtcacc
    12721 tgcagcttta gcgttaacct gttcctggaa acgttcagcc tgatcttctg cgtcgtttaa
    12781 ttcgctaaaa ccattaccga tttcacgacc accgatgaag aattcaaaac ggtcggtgat
    12841 ttctgggtta acatcattac ggcgtgccag cggtgacact tctgccggat attccgtaat
    12901 aaaggttggc tgaatcagat gtgcttctgc cacttcatca aagatctccg tgacaatacg
    12961 tcccagcccc cagcttttct ctaccgtaat accgatagat tcagctaatg ctttagcagc
    13021 atcaaaatta tccaggtcgg ccatatcggt ttctggacga tattttttga ttgcttcgcg
    13081 catggtgagt ttttcaaacg gtttgccgaa atcaaacaca tgctcgccat aagtgacttt
    13141 agtggtaccc agaacctctt gtgccagggt gcggaacagg gactctgtca gttcaatcaa
    13201 atcgtggtaa tccgcatacg ccatgtagag ttccatcatt gtgaactcag gattatggcg
    13261 aacagaaata ccttcattac ggaagttacg gttgatttcg aatacccgtt caaaaccgcc
    13321 tacaaccaga cgtttcagat acagctccgg cgcgatacgc aggtacatat ctaaatccag
    13381 agcgttatga tgggtaataa acgggcgagc agatgcccca cctggaatta cctgcatcat
    13441 cggggtttct acttccataa agccgcgcgc gaccatgaac tggcggatag cggccagaat
    13501 ttttgaacgg acaacaaacg tttgacggga tttatcgtta gcgatgaggt ccagataacg
    13561 ttgacgataa cggacttcct gatcctgcag accatggaat ttatctggta aaggacgtag
    13621 tgcttttgtc agcaggcgca actcagtaca gtgaatggaa agctcaccgg tttgcgtctt
    13681 aaacagcgta ccgcgggcac cgataatgtc acccagatcc cattttttaa actgatcgtt
    13741 ataaacacct tctggcaggc tatctcttgc aacgtacagt tgaatacggc caccgacatc
    13801 ctgcaacgtt acaaaggagg ctttccccat gatacgacgg gtcatcattc ggccagcaac
    13861 cgagacttca atgtttaagg attccagttc ctggttatcc ttcgcatcaa actcttcgtg
    13921 cagctggtca gaggtatggt cgcggcgaaa atcattggga aacgccacac cttgctgacg
    13981 tagtgccgcc agtttttcgc ggcgatttct cagttcatcg ttaaaatcaa tagcctcatt
    14041 ggctccccgt gtttcttgtt cagacatttt ggttcctcta aatccagctt tcaatttagc
    14101 gtagataaag agacagatcg gtacactgta ttatctgcct cgactattaa taactcagtt
    14161 tatcttatcg aaactaacgc ttacctcaag ttgatatcac taataaataa ccatcaaaat
    14221 ggtttaaatg acttattaat ctagatttgc tagtgttata aactgaaagt taatttattc
    14281 ataaccaatt gtttttacga cagttaatcg ttgctctcct gtatcatatt cgtcggcgtg
    14341 ctaaacagaa aacgggtagc aaaggccaat acgacgatga tcgcgacaca agccaacgtc
    14401 cactctccca tctgagaaaa gaatcgctgg taagctgcaa ttgccatacc gctaatttgc
    14461 gactctgtcg tctgctgtgc cacgacgcct gccagccagt tggcgaccgc gcctgttgcc
    14521 agcatataaa taccggttaa tacgccagac attttcagac gcgtgatttg cgcaatcgcc
    14581 accgggtcaa taaagagttc ggcaaagccc attagcgcca gcccggatat catcacggcc
    14641 attgacgctt gaccgccagc cgctgcatgt cgggcatcaa atgccaacaa cataaagcca
    14701 caagccatca gcagtaagcc aaaggcaaac ttcagccaga cgcgcaatgt tgagttgccg
    14761 cggctttctg gcgacgccag ccaggccagt acaaccccag cgagcatcac tgcaattgca
    14821 ttcaccgact ggaatagtgc tgtaggtact tcaatattga atgcctgacg attcacaaag
    14881 cgatcgataa acaagctgat ggtactgccg ccctgttgtg ccagtaccca gaacaacgtc
    14941 ccgacaaaca tcaacaatac aatttgccaa agagcacggc ggtgttcggg gaatttgatc
    15001 atcatgcggg caatgatttg tgcggcaatg aggcaaacga tcgccagcaa atatccggac
    15061 cagtcgttct ccagcagcag ggtaaaaaat actggggcta aacagagcat caccactaac
    15121 cagctccata ctggtaaggc aaatttcacg ctagtgagcg cttttttatc catactacgc
    15181 gtggactgga aatgacgatg cccgcttaag aaaatcaaca aaccgataaa catgccgcca
    15241 cccgcaaggg caaagccaac atgccatcca taccactgag cagccaggcc gcaggcgatt
    15301 ggggcggcga tagaaccgat attgcccgca gcatacagca gcgaaaaacc gccatcacgt
    15361 cgatgatcgt tctcgtcgta gagctcgcca agcaaacagc tgatgtttga tttgaataaa
    15421 ccgtagccac aaataatgat tgccagcgcc agatacaggc taaaggttga atttgtatca
    15481 atgcccagca ccacatggcc aagggtcatt aacagcgcgc cggcaatcac tgcagtgcgg
    15541 ttgccgagca ggcggtcggc aagccagccg ccgagaatag gggtaacgta aaccagagaa
    15601 gcatatgcgc tgaacaggct gatggcatgg ttatcatcaa aaccaagctg atgggtgaga
    15661 tagagaatga gtaaggcacg catgccgtaa aaactgaagt actcccagat ttggatcgcc
    15721 acgatatagt atatcgcgcg cggctgtgag ggtgttttca tgtgttctcc ttatgagcaa
    15781 aaaagggaag tggcaagcca cttcccttgt acgagctaat tattttttgc tttcttcttt
    15841 caatacctta acggtatagc ggccatcagc ctgacggtat gcaccgtgaa tatcggtttc
    15901 aaagcccgga tagtgagcgc cgatttcaca cagcatctgc aggaactcca gaaccggacg
    15961 gctttcttcg gtgatcattt cacccggcat taccagagga actcccggcg gatacggaag
    16021 gatcatattg gcgttaatac gacctaccat ttcgtcgagg taaacttctt cggtcatacc
    16081 gtgcagctct ttctggaatg cagcatacgg agtcattacc atcgtcggca gcacttcaaa
    16141 tgcgcggtac atcagatccg gcagattgtg gtgaacaatc agtttgtgga tattctgagc
    16201 cagttcctga atacgcatgt tttcatagaa ttcaggatct tcacgataca gagacggcag
    16261 catgtttttc acacgcaggt tcaggtcgaa cgcacgtttg aagtcagtca gagcacgcag
    16321 caggctcagt gctttggtct tatcgatacc gatgctgaac aggaacagca ggttatacgg
    16381 accggttttc tcaacaacga tgccatgttc gtcgaggtat ttcgccacga tgctggccgg
    16441 aataccaaag tcgctcatgg tgccgtcttt ttccatcccc ggagtcagca gggtgacttt
    16501 gatcgggtca agatacatgt gctcgttatc gatgtttttg aagccgtgcc aggtgctgtc
    16561 agaacgcagc ggccagcatt cagtcgtatc gatatgatcc ggctgccata catcaaagaa
    16621 ccagccatca gattccgttc tcagacgttt gatctcttta cggaatttga tcgcacgttc
    16681 aatggaaccg ttgatcagac gtttacctgc attgcctttc atcatcgccg cagcggtttc
    16741 agtggacgcc acgataccgt agtgcggaga agtggtggtg tgcatcatgt aggcttcgtt
    16801 aaaggtttct tcgtttacgt cacctttaac gtggatcatg gaagcctgag agaacgccgc
    16861 cagcagtttg tgagtggact gggtttcgta aatcactttc ccttctacac ggccaccgct
    16921 cataccgcat ttaccttcgt aaatcggtga gaagttggtg taaggcaccc acgcggagtc
    16981 aaagtggatg gatttcacat ccagtgtttt cttgatgaag tcggtgttgt acagcagacc
    17041 atcataggta gagttggtaa ttacagcatg taccggccag gttgcgtttg gtgtttcttt
    17101 cacgcgctta gcaatggtag cgtgctggaa ttcactctgt gggataccac caagaatacc
    17161 gtaagcgtta cgggtcgggc ggaaatagat tggcgtaaca tcgctcatca tcatcaggtg
    17221 ggtcagcgat ttgtggcagt tacggtcaat cagaatggtg ctgcctgccg gagcagagta
    17281 catgccaaca attttgttcg cagtggaagt accgttggtc accatgtagc tgcggtctgc
    17341 gttaaagacg cgagcgatat actgttctgc ttctttgtgt ggaccactgt gatccagcag
    17401 agaacccagt tcagatactg aaatggaaat atcagatttc atggtattcg gaccaaagaa
    17461 atcatagaac aggctaccta ccgggctttt ctggaatgca gtaccgccca tgtgaccagg
    17521 agtacagaaa gtatatttac cttcacgaac atatttaaac agtgctttag tcagcggagg
    17581 cagaatagtg ttgatatatt cgtcagtggt ctgcttgatc ttattagcaa tatcttcagc
    17641 agcacccagc gcatattcaa agaagctaat ctgtaaacgc aggtcattca ggcttacatc
    17701 gagagtggaa tacgtattag cgaacgcgta caacggcagg ttctcgttca ttttgctaat
    17761 ttcttcgcac agctcgagat tatatttatc ccagtcaaaa ataacgccgc acagacgcgc
    17821 attgttttcg atcagtttta ataagtcgtc acggtcgttc gggtaaacaa tctggaagtt
    17881 cagacgttca agcgcgcgat gaagttcacg gatgggttct tctttaaaat aaacccccat
    17941 gtgattcaat attgcaataa cgttcatagt catatctcca ggtaaaaaag gcccctccca
    18001 acacatggga caaaatgaaa ggaggagcct cggaaaatac ttttaattaa tgtgcgttag
    18061 acgcggtgtg gttatccatt gagtggctct ggcgctcgtg cattttgcga gcgtagaaca
    18121 tcaggataat caggctgacg atgaaggtac ctgccagctc gaaggagctt gcgcccatca
    18181 gcgcgatgaa gcagaacaca caacccagta cagagcagat caggctgaca aagttgcgga
    18241 tgttaacgcc ttcaaaacga atcaggtcaa cgcaagagta gaagtacggc agcatagtca
    18301 gcagtactgc gataccggtc agttcaccga acaggtcaga tgctttacca ccggcagagt
    18361 tcatcagagt gatgaggatc atcagggcag tcattttcac tgcagccagc agcagacctt
    18421 ttttcggaat accgttgctg tcgacttcac cataaacttt cgggaagtta ccgtcgttag
    18481 cggcacgtac acctgcctgg cctaccaaca tcatccagga gcccagagaa gtcaggcacg
    18541 caaaggcggt gaatgcagaa accagcggcg cagcccagtt accgaggatg gttgaagcac
    18601 tgattgcaaa cggagcaccg gaagctgcca ttacagaaga tggatacata ccggaaagca
    18661 cctgagtcgc agcgatgtaa acaatacctg ctaaaccagt acccagcatg gttgccagcg
    18721 gaacggtacg tttcgggttt ttaaccatac cagtacttac tgctgcggat tcaacaccca
    18781 cgaaggccca caggcagagc agaatacttt taatgatcgc atgaccatca gtggtatccg
    18841 cagtattcca gttagctgca taagttgccg catcaaacca atgccagcca acaatagcgg
    18901 tcattactac cggaataaga accagcacca gaccaatagt ggttaaacgg cttacccaag
    18961 taccgccgag catatttaca aaggtaaata cccagacgat agcaatacag gcgatacccg
    19021 ccggaacagg atcatttaat actgggaaga aggtggaaag ataagatacc gcggtaatac
    19081 caatcgccag gttaccaatc cagttagcat ggtaataaag aacacctgtc tgaaaaccaa
    19141 atgcagggga aatttctccg gcataagcaa ttgggccacc ttgttgcggg ttttttgttg
    19201 ccagtcgggc atatacatac gccagcgaca ttgcaccaat aatagagata atccaacccc
    19261 agatagcaat accaccgata cttgctaggt tcgcaggtaa taatgcaata ccgctcccca
    19321 tcatattacc ggcaacaaca ccggtacagg caaatagccc gatcttcttg gcagaactca
    19381 tgctcttctc ctaatttcat ttttgaattt ggagtccggg tcatgatgta taactatttc
    19441 ctgaccagac caaactggcg ctaagaatac tcacgaaaaa aggattaatc ctaaagatta
    19501 ggtgaaataa cacaaaagtt tctgtaagtg agaacttgag gttttttatt aacacatcag
    19561 gatcgcaagt tgatatcatg aaaagataaa cattcaatgt ttacaatgga ttgcgggata
    19621 ttctctggtt aaatttatgt aataaaaatt atgcggcaaa taaattgccg caacatatta
    19681 taccaacaga aacatacaaa aactcaacaa caaatattcc cgagcataaa tcaacccggg
    19741 agttacttat tctgaagcga gaaatttgtc gagataaggt acaacataag gaacagaagt
    19801 ctggaatata ccattttcaa tccagtaaag ggtgtttgcc cctgggcgta aattaaaggc
    19861 ggtgagatat gcatcagccg cttcccggtt catccccttc atttcataaa ccttgccaag
    19921 caacacataa tttagccagg acatttcaag atcaatgcca gtatttatcg cctggtaaga
    19981 ctcatctgtt ttacctttta ccagagcact gaccgctttt atttgatata taatggacag
    20041 gttattcaat tccggcagtg taacaatgtt gtctatttct gtgttcagtg ctgctaattg
    20101 tttttcatct aaaggatgtt gagaatggcg cacgatatca actaatgctt tttctgctct
    20161 cgcgtaggta aattctgggg atgattgaac aatctcacct aataattcac tggcacgatt
    20221 caatgattta tcatcgccat gcagtaaata atcatgtgcc tgataaaaat tagttaataa
    20281 cgcaccacga tgaggcaata tttgctggag cgtctcctgc attcgttgtg gccacggttg
    20341 gtttaacgct tttgataaac tctccagtaa atcattttga atcgccagct gattaccgtt
    20401 agtgatgaca taacgtttat ccagcatggt tgaaccatct gcattgtcta ccaattttat
    20461 cgacataaag cattgttgag cacggtattg gcgctgatta acaaacgcaa tagataatgt
    20521 tttaccggaa ctgctcggtt catcaatgtt gtagttgatt ttgtcatgca ccataaaggt
    20581 ggagaaggtg ttaagtgatg tcgccaccaa atcacccacg cctatcgcgt aagagagctg
    20641 atacggggaa ctccagctgt tacaactttt atttaccata ttaatgtcaa tatcgcgtgg
    20701 attgagcaaa atacgcgatt tgctcatagg aagacgtgta tcaagagttg aaaacgctac
    20761 cagtgctaca cagataccta acgacaacag gaaaaaaaac catacccaaa aggtagtgaa
    20821 tcgtttgctt ttaactgggg attgttcagg tggcgttgcg gtgttttgaa tgttaagact
    20881 gtgggaggga gaatctgtgg caggaaccgc ctctggtata gggggaggcg aagatagcat
    20941 tatttcctct ccctcttctt cgctgtacca gataaccggc accattaatt tatagccgcg
    21001 ctttggtaca gtagcgatat agacaggact atcttcatca ttatctttta atgacttacg
    21061 tagttctgag atactctgcg tcacaacatg attggtgaca atacttctct tccagacatt
    21121 atcgataagt tcatccctgc taagtacttc gccactgtgt tgagcaaaga aaaccagaag
    21181 atcgattaat ctcggctcaa gggtaagttg acgcccattg cggctaattt ggtttatgga
    21241 cggagtaaca agccattcgc caacgcgaac tacaggttgt tgcataatag aaactcattc
    21301 gaaaagggaa tgatgcaatg ataattgcca caacctattt ttaccatcta tagatgggtt
    21361 tatttacata ttattggtga atgcaagacg ttatttttac cagccataaa cttcttgatt
    21421 acatagtatt acgaaaggat tttactgaga accagaagta atattcctta ccatcaaaat
    21481 tcatcatctt tgccaaagaa aaatgttcag aaaataatcc atggaaaatt gtccggagcg
    21541 cttactattt taatggattg ttagtctttg catgagcaag cggactgata catttctctt
    21601 tgttctcatt cagaaaatct catcagtcgg cgttcagacc ggtgttgtgc tttatggatg
    21661 cgttacgctc ctgatgacgt catttgacgt tcaacaacat cacggggccg cacgacattt
    21721 cacgtcagtt agtgctatcg ctcaggaaca aattttcccg aattgggata tgcccgcaaa
    21781 ttgctggtga tgtggggaga atctggttga gttcgggaga attgatttgg aggcagaacg
    21841 cttaaat
//
LOCUS       DAZGLJ010000058        21705 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000058 DAZGLJ010000000
VERSION     DAZGLJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.058"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            172..900
                     /locus_tag="ACHK49_002004"
     CDS             172..900
                     /locus_tag="ACHK49_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO9262657.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKKINAVSTLITRNSEHH"
     gene            900..5750
                     /gene="ehaG"
                     /locus_tag="ACHK49_002005"
     CDS             900..5750
                     /gene="ehaG"
                     /locus_tag="ACHK49_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFO9262658.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNSSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKTTNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITASTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TSGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6119..7774
                     /gene="lldP"
                     /locus_tag="ACHK49_002006"
     CDS             6119..7774
                     /gene="lldP"
                     /locus_tag="ACHK49_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO9262659.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7774..8550
                     /gene="lldR"
                     /locus_tag="ACHK49_002007"
     CDS             7774..8550
                     /gene="lldR"
                     /locus_tag="ACHK49_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO9262660.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8547..9737
                     /gene="lldD"
                     /locus_tag="ACHK49_002008"
     CDS             8547..9737
                     /gene="lldD"
                     /locus_tag="ACHK49_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO9262661.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9923..10396
                     /gene="trmL"
                     /locus_tag="ACHK49_002009"
     CDS             9923..10396
                     /gene="trmL"
                     /locus_tag="ACHK49_002009"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO9262662.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10449..11270)
                     /gene="cysE"
                     /locus_tag="ACHK49_002010"
     CDS             complement(10449..11270)
                     /gene="cysE"
                     /locus_tag="ACHK49_002010"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO9262663.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11350..12369)
                     /gene="gpsA"
                     /locus_tag="ACHK49_002011"
     CDS             complement(11350..12369)
                     /gene="gpsA"
                     /locus_tag="ACHK49_002011"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO9262664.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12369..12836)
                     /gene="secB"
                     /locus_tag="ACHK49_002012"
     CDS             complement(12369..12836)
                     /gene="secB"
                     /locus_tag="ACHK49_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO9262665.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12899..13150)
                     /gene="grxC"
                     /locus_tag="ACHK49_002013"
     CDS             complement(12899..13150)
                     /gene="grxC"
                     /locus_tag="ACHK49_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO9262666.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13292..13723)
                     /gene="yibN"
                     /locus_tag="ACHK49_002014"
     CDS             complement(13292..13723)
                     /gene="yibN"
                     /locus_tag="ACHK49_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO9262667.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13968..15512
                     /gene="gpmM"
                     /locus_tag="ACHK49_002015"
                     /gene_synonym="pgmI"
     CDS             13968..15512
                     /gene="gpmM"
                     /locus_tag="ACHK49_002015"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO9262668.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15546..16805
                     /gene="envC"
                     /locus_tag="ACHK49_002016"
     CDS             15546..16805
                     /gene="envC"
                     /locus_tag="ACHK49_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO9262669.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16809..17768
                     /gene="yibQ"
                     /locus_tag="ACHK49_002017"
     CDS             16809..17768
                     /gene="yibQ"
                     /locus_tag="ACHK49_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO9262670.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMRALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17755..18789)
                     /gene="waaH"
                     /locus_tag="ACHK49_002018"
     CDS             complement(17755..18789)
                     /gene="waaH"
                     /locus_tag="ACHK49_002018"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO9262671.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(19040..20065)
                     /gene="tdh"
                     /locus_tag="ACHK49_002019"
     CDS             complement(19040..20065)
                     /gene="tdh"
                     /locus_tag="ACHK49_002019"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO9262672.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(20075..21271)
                     /gene="kbl"
                     /locus_tag="ACHK49_002020"
     CDS             complement(20075..21271)
                     /gene="kbl"
                     /locus_tag="ACHK49_002020"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO9262673.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(21546..>21705)
                     /locus_tag="ACHK49_002021"
     CDS             complement(21546..>21705)
                     /locus_tag="ACHK49_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HFO9262674.1"
                     /translation="DQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQA
                     LRIFLSRK"
BASE COUNT         5508 a         5640 c         5534 g         5023 t
ORIGIN      
        1 caaacacaga catcttttga aacgaatgat acatttttat gatgaatgct taaaacaata
       61 tttaatcgac acacatcact gtcattaatg taaagcaaca tagatcacca ataatacaat
      121 ggacaactct accggcattg atcgctggtc ggaggaaata aggattttta catgaacttg
      181 aagaagattt ttttcagtgc tgtcactgtt tcagttctct gcgcattaac gggatgtgac
      241 tacattgaag aaggcaagcc agaaagtagt ttattgaaac aacaagaaga acataacaat
      301 aaaattgatc tacttgaaaa acaacaagca cagctaaaga gtcaactgga aactattcaa
      361 aaacaacaaa ctggcataat aagcagtaca aagactttaa ctcacgtaat taaatccgtt
      421 aaagatcaac aaaacacttt tatttttacc gagtttaacc cagcaaaaac gaagtatttc
      481 atcctgaaca atggttcggt ggctttagcg ggtcgagtgt tatccattga cgccacagag
      541 aatggcagtg ttattcatat ttcactggtc aacttattaa gtacacctat ctcaaatatt
      601 ggttttaatg cgacatgggg tggtgaaaaa cctgtcgatg ccaaagagtt tgcccggtgg
      661 caacaattac ttttcaacac atcaatgaaa tccacattga aattattacc aggtcaatgg
      721 caagacatta atttgaccct gaagggtgta tcgcccaata acctgggata tctgaagtta
      781 gccatcaaca tggaaaatat tcagttcgac aatcttcctt ctgctgaaaa tcggcagaaa
      841 agaagcaaaa aaataaatgc agttagtacg cttataacaa ggaatagcga gcatcattaa
      901 tgaacaaaat atttaaagtt atctggaacc ctgcgacagg gaattatact gttaccagcg
      961 aaacggcaaa aagccgtggc aagaaatctg ggcgcagtaa gctgttaatt tctgcgctgg
     1021 ttgcgggtgg aatgttgtcg tcgtttgggg cattggcgaa tgccgggaat gacaacggtc
     1081 agggtgttga ttacggtagt ggatcagctg gcgacggctg ggttgctata ggcaaagggg
     1141 cgaaagcaaa tacttttatg aacaccagtg gttccagtac tgctgtgggt tatgacgcta
     1201 tagctgaagg ccaatatagc tctgccatcg ggtcaaaaac ccatgcgatt ggtggtgcat
     1261 caatggcctt tggggttagt gcaatatcag aaggcgatag aagtatagca ctgggtgcct
     1321 cttcgtattc attgggccaa tactcaatgg ccctcggccg ttattcaaaa gcattgggta
     1381 aattgtctat tgctatgggg gactcttcca aagcggaagg agcaaacgcc attgccctgg
     1441 gaaatgccac taaagctact gagattatga gtattgctct tggcgacacc gccaatgcgt
     1501 caaaagcgta ttcaatggcg ctgggagcaa gtagcgtcgc atctgaagaa aacgcaattg
     1561 ccctggggcg tagcagtgta gctagcggta ctgacagcct cgcatttggc agacaatcac
     1621 ttgccagcgc agcgaacgct attgcgatag gtgctgagac cgaagccgct gaaaatgcaa
     1681 ctgctattgg caataatgcg aaggcaaaag ggactaatag catggcaatg gggttcggaa
     1741 gccttgccga taaagtcaat actatcgcat taggaaatgg cagccaggct ctggcagata
     1801 atgcaatcgc cataggccag ggcaacaaag ctgatggcgt ggatgccatc gctctgggta
     1861 atggtagcca gtcgagaggc ttaaacacca ttgccttagg cacagccagt aatgcaactg
     1921 gtgataagag tcttgcgctt ggtagtaata gcagtgccaa cggtattaac tctgtcgcgc
     1981 tgggcgcaga ttccattgcg gatttagaca ataccgtctc tgtcggcaat agttcattaa
     2041 aacgcaagat cgttaatgtg aaaaatggcg cgatcaagtc tgacagttac gatgccatta
     2101 atggttcaca gctttatgcc attagcgact cggtagcaaa aaggcttgga ggaggggctg
     2161 cagtagatgt tgatgacggt actgttacag caccaaccta caatttaaaa aatagtagca
     2221 aaaataacgt aggggctgcg ctcgctgtac ttgatgaaaa caccctgcaa tgggaccaaa
     2281 ccaaaggcaa atacagcgct gctcatggta ctagtagccc aactgccagc gtaatcaccg
     2341 atgttgcgga tggcacgatt tcagcctcca gtaaggatgc ggttaacggt tcccaactga
     2401 aaactaccaa tgacgatgtc gaagccaaca ccgccaatat cgctactaat accagcaaca
     2461 ttgccacgaa tacggcaaat attgccacca ataccaccaa tatcaccaac ctgacggatt
     2521 ccgttggtga ccttcaggct gatgccctgc tctggaacga aactaaaaag gcattcagtg
     2581 cagctcacgg ccaggatacc accagcaaaa tcaccaacgt taaagatgcc gacctgacgg
     2641 ctgacagcac tgatgctgtt aacggctctc agctgaaaac caccaacgat gctgtggcga
     2701 cgaataccac caatatcgcc aataacactt ccaatattgc cactaacacc accaacatct
     2761 ctaacctgac tgagacggtg actaatcttg gtgaggatgc gctgaaatgg gataaggaca
     2821 atggtgtatt cacggcagct catggcaccg agaccaccag caaaatcacc aacgttaaag
     2881 atggcgacct gacgactggc agcaccgatg ccgttaacgg ctctcagctg aaaaccacca
     2941 acgatgccgt ggcgacgaat accaccaata tcgccactaa caccaccaac atctctaatc
     3001 tgactgagac ggtgactaat cttggtgagg atgcgctgaa atgggataag gacaatggtg
     3061 tcttcactgc agctcatggc aacaataccg ccagcaaaat caccaatatc ctggacggca
     3121 cagtcactgc aaccagttcc gatgccatta acggtagcca gctttatgac ttaagcagca
     3181 atatcgccac ctacttcggc ggcaatgctt ctgtgaatac tgacggtgtg tttaccggtc
     3241 caacctacaa aatcggtgaa acaaattatt ataacgtcgg cgatgcactg gctgcgatta
     3301 actcctcatt tagcacgtct ctcggcgatg ctctgctttg ggatgccacc gcaggtaaat
     3361 tcagtgccaa acacggtact aatggtgacg caagcgtgat cactgatgtc gcagatggtg
     3421 aaatttcaga ctccagttct gacgcagtaa acggctcaca actccacggc gtgagcagtt
     3481 atgttgttga tgcgctgggg ggtggtgccg aagtcaatgc agacggcacc atcactgcgc
     3541 cgacgtacac cattgctaat gctgattacg ataatgtcgg tgatgccctg aatgctatcg
     3601 ataccactct tgacgacgct ctgctctggg atgcggacgc cggtgaaaat ggtgcattta
     3661 gcgccgctca cggaaaagat aaaactgcca gtgtaatcac taacgtcgct aacggtgcaa
     3721 tctctgctgc cagcagcgac gcgattaacg gctcacaact ctataccacc aataagtaca
     3781 tcgctgatgc gctgggtggt gacgcagaag tcaacgctga cggcaccatc accgcatcga
     3841 cttacaccat tgcgaacgcc gagtacaaca acgtcggtga cgccctggat gcgcttgatg
     3901 ataacgcctt gctgtgggat gagactgcca atggcggtgc tggagcctac aatgccagcc
     3961 atgacggtaa agccagcatc atcactaatg tcgctaatgg cagtattagt gaggacagta
     4021 ccgatgcagt gaacggttct cagttgaatg cgacgaatat gatgattgag cagaacaccc
     4081 aaattatcaa tcagctcgct ggtaacaccg acgcaaccta tatccaagaa aacggtgcgg
     4141 gtattaacta tgtgcgtact aacgacgacg gcttagcgtt caacgacgcc agcgcacagg
     4201 gtgttggcgc tacagctata ggttataact ctgtcgccaa aggcgatagc agcgtagcta
     4261 ttggtcaggg cagctacagc gacgttgata cgggtatcgc cctgggtagc agctctgttt
     4321 ccagccgagt gattgccaaa ggctcccgtg acaccagcat aacggaaaat ggcgttgtta
     4381 ttggttacga caccacggat ggcgaactgc tcggtgcatt gtctatcggt gatgacggta
     4441 aatatcgtca aatcatcaac gtagccgatg gttccgaagc ccatgacgcc gttacggttc
     4501 gtcaattgca gaatgcgatt ggtgcggtcg caaccacgcc gactaaatac ttccacgcta
     4561 attcaacgga agaagattca ctggcagtgg gaactgactc gctggcaatg ggtgcgaaaa
     4621 ccatcgtgaa tggcgataaa ggtattggta tcggttatgg tgcctacgtg gacgcgaatg
     4681 cacttaacgg cattgccatt ggtagcaatg cgcaagtcat tcatgtcaac agtattgcga
     4741 taggtaatgg ttctacgacc actcgtggcg ctcaaaccaa ttataccgcc tacaacatgg
     4801 acgcaccgca gaactctgtc ggtgaattct cagtcggtag tgcggatggt caacgtcaga
     4861 tcactaacgt cgcagcaggt tcggctgata ccgatgcggt caacgtgggt cagttgaaag
     4921 taacggatgc gcaggtttcc cagaataccc agagcattac taacctgaac aatcaggtca
     4981 ctaatctgga tactcgcgtt actaatatcg aaaacggtat tggcgacatt gtcacctccg
     5041 gtagcaccaa gtacttcaag accaacaccg atggcgtaga tgccaacgcc cagggtaaag
     5101 atagcgttgc tattggttct ggttccattg ctgccgctga caacagcgtc gcactgggta
     5161 ccggttccgt tgcagaggaa gaaaatacaa tctctgtagg ttcttctact aaccaacgcc
     5221 gtatcaccaa cgtagctgcc ggtgttaatg ccaccgatgc ggttaacgtg tcgcagctga
     5281 aatcttctga agcaggcggc gtgcgctacg acaccaaagc tgatggttct atcgactata
     5341 gcaatatcac cctcggtggt ggcaacggca gtacgactcg tatcagcaac gtctccgctg
     5401 gcgtcaacaa caacgacgcg gtgaactacg cgcagttgaa gcaaagcgtg caggaaacga
     5461 agcaatacac cgatcagcgg atggttgaga tggataacaa actgtctaaa actgaaagca
     5521 agttgagcgg tggtatcgct tctgcaatgg caatgaccgg tctgccgcag gcttatacac
     5581 cgggtgccag catggcttct attggtggcg gtacttacaa cggtgaatcg gcagttgctt
     5641 taggtgtatc gatggtgagc gccaatggtc gttgggtcta caaattacaa ggtagtacca
     5701 atagccaggg tgaatactcc gccgcactcg gtgccggtat tcagtggtaa tgatccatta
     5761 agttagtgtg actaagggca aacgccaaat tgcctgatgc gttacgcgca tcaggcaaga
     5821 taaggcgctc cgggtcaaca atctaagggc aattctctga tgaggattgc ccttttcttt
     5881 accagacatc tcccccctac aagaattggc cctaccaatt cttcgcttat ctgacctctg
     5941 gttcacaatt ccccaattaa aactcacatc aatattgcca atacataaca tttagttaac
     6001 cattcattgt cattatccct acacaacaca attggcagtg ccacttttac acaacgtgtg
     6061 acaaggagat gagcaacaga ctcattacac gatgtgcgtg gactccagga gacctgcaat
     6121 gaatctctgg caacaaaact acgatcccgc cgggaatatc tggctttcca gtctgatagc
     6181 atcgcttccc atcctgtttt tcttctttgc gctgattaag ctcaaactga aaggatacgt
     6241 cgccgcctca tggacggtag caatcgccct tgccgtggct ttgctgttct ataaaatgcc
     6301 ggtcgctaac gcgctggcct cggtggttta tggcttcttc tatggtttgt ggcccatcgc
     6361 gtggatcatt attgcagcag tgttcgtcta taagatctcg gtgaaaaccg ggcagtttga
     6421 catcattcgc tcgtctattc tttcgataac ccctgaccag cgtctgcaaa tgctgatcgt
     6481 cggtttctgt ttcggcgctt tccttgaagg agccgcaggc tttggcgcac cggtagcaat
     6541 taccgccgca ttgctggtcg gcctgggttt taaaccgctg tacgccgccg gactgtgcct
     6601 gattgttaac accgcgccag tggcatttgg cgcgatgggc attccaatcc tggttgccgg
     6661 acaagtaaca ggcatcgaca gttttgagat tggccagatg gtggggcgtc agctaccgtt
     6721 tatgaccatt atcgtgctgt tctggatcat ggcgattatg gacggctggc gcggtatcaa
     6781 agagacgtgg cctgcggtcg tggttgcggg cggctcgttt gccatcgctc agtaccttag
     6841 ctccaacttc attgggccgg agctgccgga cattatctct tcgctggtat cactgctctg
     6901 cctgacgctg ttcctcaaac gctggcaacc agttcgcgta ttccgcttcg gtgacttggg
     6961 ggcgtcacag gttgatatga cgctggccca caccggttac accgcgggtc aggtgttacg
     7021 tgcctggaca ccgttcctgt tcctgaccgc taccgtaaca ctgtggagca tcccgccgtt
     7081 taaagccctg ttcgcatcgg gtggtgcgct gtatgagtgg gtgatcaaca ttccggtgcc
     7141 gtacctcgat aaactggttg cccgtatgcc accagtggtc agcgaggcta cagcctatgc
     7201 cgccgtgttt aagtttgact ggttctctgc caccggcacc gccattctgt ttgctgcact
     7261 gctctcgatt gtctggctga agatgaaacc gtctgacgct atcagcacct tcggcagcac
     7321 gctgaaagaa ctggctctac ccatctactc catcggcatg gtgctggcgt tcgcctttat
     7381 ctcgaactat tccggactgt catcaacact ggcactggca ctggcgcaca ccggtcatgc
     7441 attcaccttc ttctcgccgt tcctcggctg gctgggggta ttcctgaccg ggtcggatac
     7501 ctcatctaac gccctgttcg ccgcgctgca agccaccgca gcacaacaaa ttggcgtctc
     7561 tgatctgttg ctggttgccg ccaataccac cggtggcgtc accggtaaga tgatctcccc
     7621 gcaatctatc gctatcgcct gtgcggcggt aggcctggtg ggcaaagagt ctgatttgtt
     7681 ccgctttact gtcaaacaca gcctgatctt cacctgtatg gtgggcgtga tcaccacgct
     7741 tcaggcttat gtcttaacgt ggatgattcc ttaatgattg ttttacccag acgcctgtca
     7801 gacgaggttg ccgatcgtgt gcgggcgctg attgatgaaa aaaacctgga agcgggcatg
     7861 aagttgcccg ctgagcgcca actggcgatg caactcggcg tatcacgtaa ttcactacgc
     7921 gaggcgctag caaaactggt gagcgaaggc gtgctgctca gtcgacgcgg cggcgggacg
     7981 tttattcgct ggcgtcatga cacatggtcg gagcaaaaca tcgtccagcc gctaaaaacg
     8041 ctgatggccg atgatccgga ttacagtttc gatattctgg aagcccgcta cgccattgaa
     8101 gccagcaccg catggcatgc ggcaatgcgc gccacacctg gcgacaaaga aaagattcag
     8161 ctttgctttg aagcaacgct aagtgaagac ccggatatcg cctcacaagc ggacgttcgt
     8221 tttcatctgg cgattgccga agcctcacat aacatcgtgc tgctgcaaac catgcgcggt
     8281 ttcttcgatg tcctgcaatc ctcagtgaag catagccgtc agcggatgta tctggtgcca
     8341 ccggtttttt cacaactgac cgaacaacat caggctgtca ttgacgccat ttttgctggt
     8401 gatgccgacg gggcgcgtaa agcaatgatg gcgcacctta gttttgttca caccaccatg
     8461 aaacgattcg atgaagatca ggctcgccac gcacggatta cccgcctgcc cggtgagcat
     8521 aatgagcatt cgagggagaa aaacgcatga ttatttccgc agccagcgat tatcgcgccg
     8581 cagcgcaacg cattctgccg ccgttcctgt tccactatat ggatggtggt gcatattctg
     8641 aatacacgct gcgccgcaac gtggaagatt tgtcagaagt ggcactgcgc cagcgtattc
     8701 tgaaaaacat gtccgactta agcctggaaa cgacgctgtt taatgagaaa ttgtcgatgc
     8761 cggtagcact ggcaccggtg ggtttgtgtg gcatgtatgc gcgtcgtggc gaagttcagg
     8821 cagccaaagc ggcggacgcg catggcattc cgtttactct ctcgacggtt tccgtttgcc
     8881 cgattgaaga agtcgcgcca gccatcaagc gcccaatgtg gttccagctt tatgtactgc
     8941 gcgatcgcgg ctttatgcgt aacgcgctgg agcgagcaaa agcagcgggt tgttcgacgc
     9001 tggttttcac cgtggatatg ccgacaccgg gcgcacgcta ccgtgatgcg cattcaggta
     9061 tgagcggccc aaatgcggca atgcgccgct acttgcaggc ggtgacgcat ccgcaatggg
     9121 cgtgggatgt gggcctgaac ggtcgtccac atgatttagg taatatctca gcttatctcg
     9181 gcaaaccgac cggactggaa gattacatcg gctggctggg gaataacttc gatccgtcca
     9241 tctcatggaa agaccttgag tggatccgcg atttctggga tggcccgatg gtgatcaaag
     9301 ggatcctcga tccggaagat gcgcgcgatg cagtacgttt tggtgctgat ggaattgtgg
     9361 tttctaacca cggtggccgc cagctggacg gtgtactctc ttccgctcgt gcgttgcccg
     9421 ctattgcaga tgcggtgaaa ggtgatatcg ccattctggc ggatagcgga attcgtaacg
     9481 ggcttgatgt cgtgcgtatg attgcgctcg gtgccgacac cgtactgctg ggtcgtgctt
     9541 tcctgtatgc gctggcaaca gcgggccagg cgggtgtagc taacctgcta aatctgatcg
     9601 aaaaagagat gaaagtggcg atgacgctga ctggcgcgaa atcgatcagt gaaattacgc
     9661 aagattcgct ggtgcagggg ctgggtaaag agttgcctac ggcactggct ccgatggcga
     9721 aagggaatgc ggcatagtcg tttgcgcccc ctcaccctaa ccctctccct cagggagagg
     9781 ggaccgttcg gcgctgtatg tactccctca ctctgaaacg acaccgcact ctttttttct
     9841 ccctcgcccc tccggggtga ggggaaaagg ccgcactgct caccaatttt ctgctatcct
     9901 gcccccgcac taagggggca gtatgctaaa catcgtactt tacgaaccag aaattccgcc
     9961 aaatactggc aatatcatcc gtctttgcgc taataccggc tttcgtctgc atatcatcga
    10021 accgatggga tttgcctggg acgataagcg cctgcgccgc gcggggctgg actatcacga
    10081 gtttaccgcc gttacgcgtc atcatgactt tcgcgcgttc ctcgaagcag aaaatcccca
    10141 gcgcctgttt gccctcacta cgaaaggtac gcctgctcac agcgccgtaa actatcagga
    10201 tggcgattat ctgatgttcg gcccggaaac acgcggcctg ccagcgagca ttcttgatgc
    10261 cctgcccgct gaacaaaaaa ttcgcattcc aatggtgccg gacagccgca gcatgaacct
    10321 gtccaatgcg gtgtcggtag tggtgtatga agcctggcgg cagctgggat atccgggagc
    10381 gttgttgaga gattagttac tgtatgccgg atagatgatt acatcgcatc cggcacgatc
    10441 acagcacatc agatcccatc cccatactca aatgtatggt taataccgtt gaaatgctgg
    10501 tccatatcca ttgatggctt atcgctgtct ggtttaccga caatacgagc cggaacacca
    10561 gcggcggtag tatgcggcgg caccggttgc agcaccacgg aacctgcgcc aatcttcgcg
    10621 ccgcgaccaa cttcaatatt gccgagaatt ttcgcgcccg cgccaatcat cacaccttca
    10681 cgaattttcg ggtgacggtc accaccagat ttacccgtac cgccaagcgt cacagattgc
    10741 agaatcgata cgtcgttttc aattaccgcc gtttcaccaa cgacgatgcc cgtggcgtgg
    10801 tcgagcatga taccgcgacc aatttttgct gccgggtgaa tatcgacctg gaacgtcaca
    10861 gaaacctggt tttgcagaaa gattgccagt gcgcgacgcc cctgattcca caaccagtga
    10921 ccgatgcgat aggcctgcaa ggcatgaaaa cccttcaggt ataacaacgg ggttgagtat
    10981 ttatcgactg ccgggtcgcg ggtacgcacc gcctgaatat cacaggccgc agaggcgatc
    11041 atttccgggt cagcggcgta ggcttcttca accacttcac ggatagcaat agcaggcata
    11101 attggcgatg acagcttgtt cgccagcatg tagctcaggg cactgccaag gttttcgtgc
    11161 ttgagtagcg tcgcgtggta aaaactggcc agcattggct cacagtccgc cagcgttctg
    11221 gcttcggctt taatattgtt ccagacaatt tccagttctt cacacgacat tgcttactcc
    11281 acacgatgag ataatgaccg gcccgttctg cgcgggccgg gtcatagcgg taacaaaggt
    11341 tccctggggt tagtggctgc tgcgctcgtc cttgcgtgca cgacctagta aagtcaatgc
    11401 tgcctcgcgc gcgttttttc cgcaatataa tacttgataa atttcctcgg ttattggcat
    11461 ttcaacgccg aagcgatgcg ccagttcgcg gacttctttc gtattgcggt agccttccac
    11521 cacctgacca atcttctcct gcgcgctttg tacatccatg ccctgaccga gcatcatgcc
    11581 aaaacggcgg ttacgcgact ggttgtcggt acaggtaagc accagatcgc ccagccccgc
    11641 catgcccata aaggtggcag gatcggcacc cagcgccgca ccaagacgcg acatttcagc
    11701 cagcccacgg gtgatcagcg ccgtacgcgc attcgcacca aaaccgatac cgtcagacat
    11761 ccccgcgcca atggcaatga cgtttttcac cgcgccgcca agctgcacgc caatgaaatc
    11821 cgggttgctg taaacgcgga aacttttgcc acagtgcagc aattgttgga gatcatcggc
    11881 aaaggtctgg tcggtagagg ccaacgaaat agccgtcggt aaacctgccg ccagttcttt
    11941 cgcaaacgtt gggccagaga taaccgccag cggaatttga tcgcctaagg cctcacgcgc
    12001 cacgtcctgt aacagacgtc cggtttccgc ttccagccct ttggtcgccc acaccagacg
    12061 cgcatcagga cgcatcagcg gtttaatctg gcgcagcact tcaccaaaga catggctggg
    12121 tacgacgacg agaatattac ggctggctgc cagcgcagtg gcgagatcgc tttcaagatg
    12181 gagcgtatcg ggaaaaggca catcggggag aaacgcggcg ttacagcggt cgcgttcaag
    12241 cgttgcgata tgttcagggt catggcccca gaggacaacc tcgtggccat ttcttgccag
    12301 ggtaatggca agagcggtgc cgtacgagcc ggcaccgatc acagtcattg aagcattacg
    12361 ttggttcatc aggcatcctg atgttcttca gtaccttcgc cagcctgctg ctgcaaatag
    12421 ttcatgaaca gcgcatcgaa gttaaccggc gcaaggttca gttgcgggaa tgtaccgcgg
    12481 gataccatgc tggtgatgca ctcacgagca tacgggaaca gaatgttcgg gcagtatgcg
    12541 cccaggcaat gtgccatctg ggtgccttcg atacccgcga tggagaaaat accgccctgc
    12601 tgaacttcac acaggaacgc ggtttcttcg cccagggagg ccgttacggt aacacgcagt
    12661 accacttcgt atacgtcatc tgccagttgg gaagatgccg tatccagatc aagtttaact
    12721 tctggttgcc aatctttctg gaaaacgtgc ggcgcgttcg gcgcttcgaa agagatatcc
    12781 ttggtataaa tacgttggat ctggaaagtc atttcagtgt tgttttgttc tgacatgtgt
    12841 agaaaaccct taagtgttgt ccttaaatac tgcgcaatgt aatgccaacg ttcacacgtt
    12901 atttcagcag gggatccagt ccaccacgtg catccaatgc atacaagtca tcacagccgc
    12961 caatgtgctg tgcgtcaata aaaatctggg gaaccgtggt gcgaccgctg cgtttgatca
    13021 tctcttcacg cttggcggca ttgccatcga tcggcaactc ctgaaaactc acgcccttgc
    13081 tgctcagcag tgcttttgca cgatggcaat acgggcaggt ttctttggta tagatttcaa
    13141 cattggccat aatttagctc ctgttttttt accctgaaat ttcatgttgc agggcggctg
    13201 caaaatggag tgtccccaga agcttataac aataagtcgc tgggggccta attgcagcta
    13261 acggcctgca tcatgaaaga cgacaggtaa attatttgcc gcgcaccaga ggcaagtttt
    13321 cgccagccca gccagcaacg ccctctttca gcacgaatac ttgcgcaaaa ccagctttcg
    13381 tcagcgcgtt tgcaggctcc tggcactgca tgccagaacc gtctaccacg ataaccggtt
    13441 tgtctttgtg cttctcaagc tcaccaacat tgttggcttt gatttcactc ggcaacagat
    13501 tgatagaacc tgcgatatgg cctttacgga agtcatcacg ctgacgtaaa tccacaacca
    13561 cagcgtcttc tttgttgatc agacgcgtag cttcaccacg agtaatcacc ttcactttcg
    13621 aggtcaggct cttaaacgta gtcacaagaa ccgccaccag taacgcgatc caggcgatac
    13681 tcagtatggg atgacggcca acaaattgca taatttcttg catggggggt aacaactccc
    13741 gacgtagtga ttaaaaaaac caggaaagga gtatacctgc gcggtgcggc aaatacagcc
    13801 agcgcgttaa ctggaatgca attttgcggg gcacgacgaa aaaaaagcgc aaatctggca
    13861 tcggcttgcc gcgagcggtc gtatattttg atcttcagag gctattttat cgattcagct
    13921 gtagtaaaat tacgcaaatt ttgactcttg agtatgaggt tgtcgcaatg tcggtttcta
    13981 aaaaacctat ggtactggtg attctggatg gctatggcta tcgtgaagaa cagcaggata
    14041 acgccatttt tagtgctaaa accccggtaa tggatgcact gtgggccaat cgtccgcata
    14101 ccctgatcga cgcttccggt ctggaagtcg gtctgcctga ccgtcagatg ggtaactccg
    14161 aagtaggcca cgttaacctg ggtgccggcc gcatcgtgta tcaggacctg actcgtctgg
    14221 acgttgaaat caaagatcgc gctttctttg ctaatccagt gctgactggc gcggtagata
    14281 aagcgaaaaa cgcaggtaaa gcagtacaca ttatgggtct gctctccgca ggcggcgtac
    14341 acagccacga agatcacatc atggcgatgg tagaactggc agctgaacgc ggcgcagaaa
    14401 aaatctacct gcacgcattc cttgacggtc gcgacacccc gccgcgcagt gctgaatcct
    14461 cgctgaaaaa atttgaagag aaatttgctg cgctgggtaa aggccgcgta gcgtccatca
    14521 ttggtcgtta ctacgcgatg gaccgcgata accgttggga tcgcgttgaa aaagcctatg
    14581 acctgctgac tctggcgcag ggtgagttcc aggccgatac tgccgttgct ggtttgcagg
    14641 cagcttatgc tcgcgacgaa aacgacgagt tcgtgaaagc gaccgttatt cgtgcagaag
    14701 gtcagccaga tgcggcaatg gaagacggcg atgcgctgat tttcatgaac ttccgtgctg
    14761 accgcgcgcg tgaaatcact cgtgcattcg tgaacgctga tttcgatggc ttcgcgcgta
    14821 agaaagtggt taacgtcgat ttcgtgatgc tgaccgaata cgccgctgac atcaaaactg
    14881 cggttgctta cccacccgct tccctggtta acaccttcgg cgagtggatg gcgaaaaacg
    14941 acaaaactca gttgcgtatt tccgaaaccg aaaaatatgc ccacgttact ttcttcttca
    15001 acggtggcgt agaagagtcg ttcaaaggcg aagatcgcat tctgatcaac tcaccgaaag
    15061 tggctaccta cgatctgcaa ccggaaatga gctccgcaga gctgaccgaa aaactggttg
    15121 cggccatcaa gagcggcaaa tacgacacca ttatctgtaa ctatccgaac ggcgacatgg
    15181 taggtcacac cggggtgatg gaagcggcgg ttaaagcggt tgaagcactg gatcactgcg
    15241 tggaagaagt cgcgaaagcg gttgagtccg tgggtggaca actgctgatc accgctgacc
    15301 acggtaacgc tgaacagatg cgcgatccgg caacgggtca ggcacacacg gcacacacca
    15361 acctgccagt tccgctgatt tatgttggtg ataagaacgt gaaagcggtt gaaggcggca
    15421 aactttctga catcgcgccg accatgttgt cgctgatggg tatggaaatc ccgcaagaga
    15481 tgactggtaa gccgctgttc atcgtggaat aatccctccc catgagggga aaggcgatta
    15541 ataccatgac acgggccgtg aaaccgcgca ggtttgcaat caggcccatc atctacgcca
    15601 gcgttcttag cgctggcgta ttgttgtgcg ccttttccgc ccacgcggat gagcgtgacc
    15661 aactcaaatc tattcaggcc gatatcgccg caaaagagcg cgcggtacgc caaaagcaac
    15721 aacaacgcgc aagcctgctc gcacaattga aaaagcagga agaagcgatc tctgaagcca
    15781 cccgtaagct gcgcgaaacg caaaacacgc tcaatcaact caataaacag attgatgaga
    15841 tgaacgcgtc gattgccaaa ctggagcagc aaaaagccgc ccaggagcgc agcctcgccg
    15901 cgcaactgga tgccgcattc cgtcagggcg agcataccgg tattcagcta attctcagcg
    15961 gtgaagaaag ccagcgtgga cagcgtttac aggcttattt cggctatctc aaccaggcgc
    16021 gacaagaaac cattgcccag ttgaagcaaa cgcgtgaaga agtcgccatg cagcgtgctg
    16081 aactggaaga gaaacaaagc gagcaacaaa cgctgttata tgaacagcgc gcccaacagg
    16141 cgaaactgac tcaggctctg aacgagcgta aaaagacgct ggcggggctg gaatcttcca
    16201 tccagcaagg tcagcagcag ctaagtgaac tgcgtgccaa cgaatcccgt ctgcgtaaca
    16261 gcattgcccg tgcggaagcc gcggcgaaag cgcgtgctga acgagaagcg cgtgaggccc
    16321 aggcggttcg cgaccgccag aaagaagcga cgcgcaaagg caccacctat aaaccgaccg
    16381 aaagcgaaaa atcgctgatg tcccgaactg gtggcctggg tgcgccgcgt ggtcaggcat
    16441 tctggccggt tcgcgggccg acgctgcatc gctatggtga acagctacag ggcgaactac
    16501 gctggaaagg aatggttatc ggtgcctctg aaggtactga agttaaagcg attgccgacg
    16561 gtcgggtgat tctggctgac tggctgcaag gttacggtct ggtggtggtg gttgagcacg
    16621 gtaaaggcga catgagtctt tacggctata atcagagcgc actggtgagc gttggttcgc
    16681 aggttcgcgc gggccagcca attgcactgg tgggcagcag tggcggtcag ggtcggcctt
    16741 cactctattt cgaaattcgc cgccagggtc aggcggtcaa tccacagccg tggttgggaa
    16801 gataagtttt gtttccattt cgtcgtaacg ttcttgcatt tgccgctctg ttggcgctct
    16861 cctcccccgt acttgctggc aaacttgcca tcgtcattga tgattttggg tatcgcccgc
    16921 acaacgaaaa ccaggtgctg gcgatgccct ccgctatctc cgtcgctgta ttacccgatt
    16981 caccgcacgc cagagagatg gcgaccaaag cgcataacag cgggcacgaa gtgttgattc
    17041 atctcccgat ggcaccactg agtaaacagc cgctggagaa aaatacgctg cgcccggaga
    17101 tgagcagcga cgaaattgag cgcattattc gtagtgcggt caataacgtg ccctatgccg
    17161 tggggatcaa caaccacatg ggtagcaaga tgacctctaa cctgtttggt atgcagaaag
    17221 tgatgcgggc actggagcgt tacaatcttt acttcctcga cagcgtaacc atcggtaata
    17281 cccaggcgat gcgcgccgcg caaggtactg gcgtgaaggt gatcaaacgg aaggtgttcc
    17341 tcgacgattc gcagaacgaa gcggacatcc gtgtgcaatt taatcgcgca attgatctgg
    17401 cgcgtcgcaa cggttcaacc attgccattg ggcatcctca cccttcaacg gtacgcgtgc
    17461 tacaacagat ggtttataac ctgccgccag acattacgct ggtgaaagcc agcagcttgc
    17521 tgaatgaacc gcaggttgat acttctacac cgccgaaaaa cgctgtgcct gatgcaccgc
    17581 gtaatccgtt ccgtggcgtg aagctgtgca aaccgaagaa accgatagaa cctgtttatg
    17641 ctaatcgctt ctttgaagta ttaagcgaaa gcatcagcca gagcacgctg atcgtttact
    17701 tccagcatca gtggcaaggc tggggcaaac agcccgaagc ggcgaagttt aacgctagcg
    17761 caaattaaag gcgctacgcg taatgcgatg gtgcgaccgc gttttgtcgc gccattgcca
    17821 taagcggaaa gaccacagta acgcctggta accgaccttc acgctgcgca cattggtaat
    17881 caggcgctta tacataccgg aagtaaatat ctcggcaatc atccgttggc gggtaagaat
    17941 atccggctct ttgcgcaccg catggcaaac acgcaatgct tcgtaagtta tttgctgatg
    18001 aaattccgga taaatcgtaa ttttgtcggc ataatttcga tttaatttct ccagcaggcg
    18061 ggtaatctta atatagtgac gttgataatt aaggttttta ttcccttgtc tatgcaaccg
    18121 actcactgac gtattatgca gataatattt atataatgat tgctcggtat atcgcgcacg
    18181 cagcgcgtta aacatgaatt ctgttgtcca gacaatatcc tgatgatgta atccggcaat
    18241 aaatttaatg ttatttttaa caataacatc acggcgataa acccccatcc agacaacgtg
    18301 agtccaacgg cgcgaagaaa gccccatccg cagccagtcc gggccggtta atacgccggt
    18361 tgagcgaagg cgatcgctgg ggatggattg ccaggtttct cccgtttcac gaaaacacca
    18421 gtcagcgttg cactgcgcca cgtcgaggtc atcctctaac gccatggtca tcagcgtttc
    18481 gtacatggtg ggatagacct catcgtcagc atcgacaaaa gcgacatatt tgcccgtcgc
    18541 cacctcgatc ccacgattac gcgctaccga tgcgccagca ttcgcctgat gcaacaaacg
    18601 aacgtgcgga tagttttctg cgtaatgctt tgctatttca acagaattat ccgttgaacc
    18661 atcgttaata ataatgattt ccagagcagt ccaggtttgc gtaattaaag attccataca
    18721 agtgcggaaa tcatcgcccg cattatataa cggaataata acactaagtt tattggtgct
    18781 gttcatcata aattaccgac gacctctgaa gttatcctga cgttttacag gctttgtatt
    18841 aagaaactat taagcctgtg agatattatt tttacaatta tttttacagg tgagagagtc
    18901 acttcagggt agagagataa tgagagcagc tatataagag atcagcgcaa ttacgatatc
    18961 tttattacac tgacaggatt ttacatattt aacggattat cctcaggcaa aagggctgga
    19021 ataccagccc ttgttcgtgt taatcccaac tcagaattac tttcccggac tggcccgaac
    19081 gcatagcgtc aaagcccttc tggaaatcat cgatagagaa acgatgggta atgatcggcg
    19141 agagatccag acctgactga atcagcgccg ccatcttgta ccaggtttca aacatctcac
    19201 gaccgtaaat acctttaatg aacaagcctt taaagattac tttggtccag tcgatagaca
    19261 tatcggacgg tggaataccc agcatcgcaa tacgaccgcc gtggttcatg gtatcgagca
    19321 tggtacgaaa cgctggcggc gcgccggaca tttccagacc gacatcaaag ccttcggtca
    19381 tgcccagttc agccatcaca tcattaaggt tttctttcgc gacgttaacc gcacgggtga
    19441 tacccatttt gcgcgccagc tcaaggcggt attcgttaac atcggtgatc accacattgc
    19501 gtgcaccaac gtgtttcgcc accgccgcag ccataatacc tatcggacct gcaccagaaa
    19561 ccagcacatc ttcgcccacc agatcgaacg acagcgcggt atgcacggcg ttaccgaagg
    19621 gatcaaaaat ggaagccagg tcgtcggaga tattgtctgg gattttgaag gcgttgaacg
    19681 ccgggatcac cagatattcg gcgaagcagc ccggacggtt aacgccgacg ccgatcgtgt
    19741 tgcggcacag atgggtacga ccgccacgac agttgcgaca gtgaccgcag gtgatatggc
    19801 cttcgccaga aacgcgatcg ccgattttga agcctttcac ttcctgacca atacctacca
    19861 cttcaccgac atattcatgg cctacgacca tcggcaccgg gatggttttt tgcgaccact
    19921 catcccagtt atagatgtga acgtcagtcc cgcagatggc tgttttacgg attttaatca
    19981 gcagatcgtt atgcccgagt tccggtacag gaacgtcggt catccagatg ccctcttccg
    20041 ctttcagttt ggataacgct ttcatctcac atcctcaggc gataacgccc agttgtttac
    20101 caatacgcgt gaatgcttct actgcacgcg taatttgctc aggggtatgc gccgcagaca
    20161 tctgggtacg aatacgcgcc tgacctttcg gaacgaccgg atagaagaaa ccggtgacgt
    20221 aaatgccctc tttttgcagc tcacgggcaa atttctgcgc cactactgca tcgccaagca
    20281 tgaccggaat aatggcatga tcggctcccg ccagggtaaa gcccgccgcc gacatttgct
    20341 cacggaactg gcgcgcgttc gcccacagac ggtcacgcag ttcgctgccc gcttcgacca
    20401 tctccagtac tttgatggac gcggcaacaa tggccggtgc cagcgagttg gagaacaggt
    20461 acggacgaga acgctggcgc agccactcaa ccacctcttt gcgcgccgcg gtataaccac
    20521 cagaagcccc gcccagcgct ttaccaagcg taccggtgat aatatcgacc cggcccatca
    20581 catcgcagta ttcatgggaa ccacgaccat tttcaccgac aaaaccgacc gcgtgggagt
    20641 cgtctaccat caccagggca tcgtatttat ctgccagatc gcaaacgccc ttcagattgg
    20701 caatcacgcc gtccatcgag aatacaccat cggtggcaat cagcacatga cgcgcaccgg
    20761 cttcacgcgc ttctttcaga cgagcttcaa gctcctgcat atcgttgttg gcatagcgat
    20821 agcgtttagc tttacacaga cgcacaccat caataataga cgcgtggttc agtgcgtcgg
    20881 agataatggc gtcttccgcg cccagcagcg tttcaaacag gccaccgtta gcatcaaagc
    20941 aggaagagta gagaatcgca tcttccatcc ccaggaaggc cgccagtttt tgttcaagct
    21001 ctttatggct gtcctgagtg ccgcaaataa aacgcaccga agccatgccg aaaccgtgag
    21061 aatccattcc cgcctttgcc gccgcaatca gatcaggatg attcgccagc ccgagatagt
    21121 tgttggcaca aaagttaatg acgtggcttc catcagccac agtgatatct gcttgctgcg
    21181 cagacgtaat aatgcgctct tctttaaaca acccttccgc ccgtgcggtt tccagatcgt
    21241 tggttaactg ctgataaaat tctccacgca ttgcgattct ccagacaggg caaattccag
    21301 cacatattac ccaaacttat aggtcgcgac gagatgacgc gttaacactt ctgcaaaatt
    21361 caggataaat aacgtgtaac ttgatgtctc tcaacaccta cagatagtag aaaaactgta
    21421 cagttttggc tgggcataat gatgattgat tttactgcat tgatataaaa agaaaaaatg
    21481 atgccaaagc atcatttttc ctatctcagt tggtatcaaa acgctaccca ctaatggagg
    21541 gaaacctatt ttcttgaaag gaaaattctc agcgcctgca tttttgcccc taaagtgtta
    21601 ctcctgaagc agcgaaacaa atcgaaccat tttcctctcc ccagatagtt taggttgaag
    21661 agatcggggc gtttgtaata gcacatgaca aaaatgccct ggtca
//
LOCUS       DAZGLJ010000059        21638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000059 DAZGLJ010000000
VERSION     DAZGLJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.059"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..307
                     /gene="cybC"
                     /locus_tag="ACHK49_002022"
     CDS             <1..307
                     /gene="cybC"
                     /locus_tag="ACHK49_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO9262675.1"
                     /translation="NMETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLE
                     DKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(352..816)
                     /gene="nrdG"
                     /locus_tag="ACHK49_002023"
     CDS             complement(352..816)
                     /gene="nrdG"
                     /locus_tag="ACHK49_002023"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO9262676.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QTFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(975..3113)
                     /gene="nrdD"
                     /locus_tag="ACHK49_002024"
     CDS             complement(975..3113)
                     /gene="nrdD"
                     /locus_tag="ACHK49_002024"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO9262677.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(3507..5162)
                     /gene="treC"
                     /locus_tag="ACHK49_002025"
     CDS             complement(3507..5162)
                     /gene="treC"
                     /locus_tag="ACHK49_002025"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO9262678.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPIYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(5212..6633)
                     /gene="treB"
                     /locus_tag="ACHK49_002026"
     CDS             complement(5212..6633)
                     /gene="treB"
                     /locus_tag="ACHK49_002026"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO9262679.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(6752..7702)
                     /gene="treR"
                     /locus_tag="ACHK49_002027"
                     /pseudo
     CDS             complement(6752..7702)
                     /gene="treR"
                     /locus_tag="ACHK49_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
     gene            7884..7937
                     /gene="mgtL"
                     /locus_tag="ACHK49_002028"
     CDS             7884..7937
                     /gene="mgtL"
                     /locus_tag="ACHK49_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO9262680.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            8078..10774
                     /gene="mgtA"
                     /locus_tag="ACHK49_002029"
     CDS             8078..10774
                     /gene="mgtA"
                     /locus_tag="ACHK49_002029"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO9262681.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(10957..11310)
                     /gene="yjeI"
                     /locus_tag="ACHK49_002030"
     CDS             complement(10957..11310)
                     /gene="yjeI"
                     /locus_tag="ACHK49_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO9262682.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11448..13094)
                     /gene="groL"
                     /locus_tag="ACHK49_002031"
     CDS             complement(11448..13094)
                     /gene="groL"
                     /locus_tag="ACHK49_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO9262683.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13138..13431)
                     /gene="groS"
                     /locus_tag="ACHK49_002032"
     CDS             complement(13138..13431)
                     /gene="groS"
                     /locus_tag="ACHK49_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO9262684.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13707..14963
                     /gene="yjeH"
                     /locus_tag="ACHK49_002033"
                     /pseudo
     CDS             13707..14963
                     /gene="yjeH"
                     /locus_tag="ACHK49_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
     gene            complement(14979..15455)
                     /gene="fxsA"
                     /locus_tag="ACHK49_002034"
     CDS             complement(14979..15455)
                     /gene="fxsA"
                     /locus_tag="ACHK49_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO9262685.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            15792..17228
                     /gene="aspA"
                     /locus_tag="ACHK49_002035"
     CDS             15792..17228
                     /gene="aspA"
                     /locus_tag="ACHK49_002035"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO9262686.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17346..18647
                     /gene="dcuA"
                     /locus_tag="ACHK49_002036"
     CDS             17346..18647
                     /gene="dcuA"
                     /locus_tag="ACHK49_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO9262687.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            18763..19101
                     /gene="cutA"
                     /locus_tag="ACHK49_002037"
     CDS             18763..19101
                     /gene="cutA"
                     /locus_tag="ACHK49_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO9262688.1"
                     /translation="MLDEESSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            19077..20774
                     /gene="dsbD"
                     /locus_tag="ACHK49_002038"
     CDS             19077..20774
                     /gene="dsbD"
                     /locus_tag="ACHK49_002038"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO9262689.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEVKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            20811..21386
                     /gene="yjdC"
                     /locus_tag="ACHK49_002039"
     CDS             20811..21386
                     /gene="yjdC"
                     /locus_tag="ACHK49_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9262690.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            21493..21568
                     /locus_tag="ACHK49_002040"
     tRNA            21493..21568
                     /locus_tag="ACHK49_002040"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21526..21528,aa:Phe,seq:gaa)
BASE COUNT         5046 a         5629 c         5578 g         5385 t
ORIGIN      
        1 caatatggaa accctcaacg acaatttaaa agtgatcgaa aaagcggata acgcggcgca
       61 agtcaaagac gcgttaacga agatgcgcgc cgcagcgctg gatgcgcaaa aagcaacgcc
      121 gccgaagctc gaagataaat caccggacag cccggaaatg aaagatttcc gccacggttt
      181 cgacattctg gtcggtcaga ttgacgacgc gctgaagctg gcaaatgaag gtaaagtaaa
      241 agaagcgcag gctgctgcag agcaactgaa aacgacccgc aacgcctatc accagaagta
      301 tcgttaattc ctcatttccc tgttgcctgc actcaggtaa cagggaactt atcatcgcaa
      361 atgatgcacc acctgattgc tgctgccgcg ccagatcagg gaagggtctt ttaaatcctg
      421 cacaaatttg ccgtcgacca gtacgttaat caaatcaaca acctgcattt gcgcagcgtt
      481 gagttcgtcg agtttatagc ctgtccacac ccagatgtct ttacccggac actcggcgcg
      541 gatgcgtttt accagtttca gaatatccgg cacgttttgc ggatgcagcg gatcgccgcc
      601 ggagagggaa ataccctggc gtttgatacg agtgtcattc agatcgttaa taatctggtc
      661 ttccattgct ttagtaaatg tctgaccgga atttacccgc catgtgcttt tgttatagca
      721 accggggcat tcatgaacac acccggagac aaacagggtg cagcgagtgc cggggccgtt
      781 gacgatgtcg acaggatagt actgatgata attcattgtt tcgcctggct tatcagtccg
      841 cgtataggat gcatccggta atcaacgcct gatgcgacgc tgtcgcgtct tatcagacct
      901 acaatcgttg ttgccatgta ggccggataa ggcgtttacg ccgcatccgg catttgctta
      961 acgtagagtg aagattaacc tatctgccca ttccccaaat gtttaacgcg gcgcttaact
     1021 tcttcctgct taccggcgtt aaatggacgt gcatccgggc tacctaaata tccgcacacg
     1081 cggcgagtca ccgacacacg ggaggcgtca tggttaccac atttcgggca agtgaagcct
     1141 ttgctggtgc actcgaactc accggtaaag ccacactcgt agcactcatc aatcggtgta
     1201 ttggtgccgt aatacggtac atgctgatag ctgtaatccc agacatcttc cagcgccttc
     1261 aggttgtgct gaatgtttgg atactcgccg tagcaaatga aaccaccgtt cgccagcggc
     1321 gggtaaggcg cttcaaagtc gatcttgtcg tacgggttca ccttcttctc cacatcgagg
     1381 tggaaactgt tggtgtagta acctttatcg gtcacgcccg gcaccacgcc aaactcagca
     1441 gtatcgagac ggcagaagcg gtcgcacagg ttttcactcg gcgtgctgta gagactgaaa
     1501 ccataacccg tttcttcttt ccactgatcc actgcctggc gcagacgttc aacaatggcg
     1561 atacctttcg cgcgaagctg ctcgttgtcg taaacatgct cgccgccgaa cagcgcgtta
     1621 atggtttcgt ggatgccgat gtaacccagc gaaatagacg cacgaccgtt tttgaagatt
     1681 tcagaaacat catcgtcagc attcagacgt acgccacaag caccttccat atagaggatc
     1741 ggggccacgc gcgctttcac gccttcgaga cgagcgatac gggtcatcag cgccttacgt
     1801 gccagcacca gacgttcatc cagcagcttc cagaaggtgg cttcatcgcc ttttgcttcc
     1861 agagcaatac gcggcaggtt caggctgatc acgccgaggt tgttacgacc atcgtggatc
     1921 tgctcgccgt tttcattttc ccacacgccg aggaagctgc ggcagcccat cggggtttta
     1981 aacgaaccgg tgactttcac tacctgatcg tagttcagga tatccggata catgcgcttg
     2041 cttgcgcact ccagcgccag ctgtttgatg tcgtagttcg gatcgccttt tttatggttc
     2101 agaccatcgc gaatcgcaaa caccagtttc gggaacaccg cagttttacg gtttttaccg
     2161 aggcctgcga tacggttacg caggatcgat tcctgaatca ggcgcgattc ccagctggtg
     2221 cccagaccaa aaccaaaggt tacaaacggc gtctgaccgt tggcggtgtg cagggtgttt
     2281 acttcgtact ccagcgactg gaaggcatcg tagcactctt tgatggtacg agagtttgcg
     2341 tagccttcgg cgtccgggat gttccactct tctgcggtct tgcgatgttt gttgtagctg
     2401 gcggtaacaa acggcgccag cacttcatcg atacggttaa tggtggtgcc gccataaata
     2461 tggctggcga cctgagcaat aatctgcgca gttaccgcgg ttgccgtaga gatcgacttc
     2521 ggcggttcaa tctcggcgtt ccccatctta aagccctggg tcagcatgcc tttcaggtcg
     2581 atcaacatgc agttgaacat cgggaagaac ggcgagtaat cgagatcgtg atagtgaata
     2641 tcgccacgct catgtgcctg caccacgtca cgcggcagca ggtgctgacg tgcatagtgt
     2701 ttagccacga tcccggccag caggtcgcgc tgggttggaa tcaccttgct gtctttgttg
     2761 gcgttttcgt tgagtaacga ggcgttggtc tgctcgacca gaccacggat ctcctggttc
     2821 aggcgaccgc gtttttcacg ttcaatgtcg cgatcgtgac ggtactcgat gtaagcacga
     2881 gccagttgtt tgtatggacc cgacatcagc tggttttcaa ctgcggtctg gatctcgttg
     2941 atatccacct ggttgcggcc ctgcatctgc tcgctgacaa ccgcggcaac agtggcgcaa
     3001 taatcggcat catcgacttc cgctgcttta gctgcacgca gaatcgcttc tttgatgcgc
     3061 tctgatttaa acggcacttt gcagccgtct cgtttcatca catgcggtgt catgatcgct
     3121 ccatattttt aagaacaggt tatccacaga aattgggaaa ggcattttcc ggttttttcg
     3181 tttcgttttc cggtgctttc cgcaatcgcc atccgcttat ccacaacgcc gcaccttttt
     3241 cgtggggagc tgtagtagca attatagtcg attaatacaa catattgggt tgggacgcat
     3301 tttaaagtct atatatagtg ctttgcatca aggatgtttt aacttttttt gatgtagctc
     3361 aaagtaaaaa gcagagcgta cggatgacgg gcgctacagc gttatgtaaa ttttttaatg
     3421 aatttgctgg ttgaaaaatc aacaaaaaca acatgctgac agacaaaacc ccggaatgac
     3481 cggcattacc ggggcttagg gaagatttac ttctgtaacc accagacagc ctcaaaaggc
     3541 cgtaaattca tggcacaggg ttgtggtgag gcttcttcgt agttatgcat cacaagctgc
     3601 cagttgccgc gcatttgccc tggctgccag ggttggatct cacggctaag gttggcaatg
     3661 accagcaagg tttgcccctt ccattcacgg cgatagcacc acaatacagg gctgtttggc
     3721 agcagatcct ggtaattgcc ccatgtcagg atggcttctt gcttacgcag tgcgattaac
     3781 ttttggtagg tgtaaaacac cgaggagtca tcggccagcg cggcttctac gttgatttgt
     3841 tgatagttat cgcccaggcc aatccacggt tcgccagccg taaacccggc attatcgccg
     3901 ttgctccatt gcatgggcgt gcggctgttg tcacgggatt tactggcgag gattgccaat
     3961 aactcgtcgg catcacgacc atcgttgcgc agctcggcaa acatattgag gctctccacg
     4021 tcgcgatagt cagtaatgcg cgtgaaatgc gggttggtca tgccaatctc ttcgccttgg
     4081 tagatatacg gcgttccctg catgccatgc agcaccatcg ccagcatttt tgccgcaggc
     4141 acgcggtatt caccttcatc accaaagcga gaaacaatgc gcggctgatc gtggttacac
     4201 cagaacaagg cattccatgc cacgttgtgc attccttgtt gccagtggcg gaacaatgtt
     4261 ttcaacgcca caaagtcagg tttagccagc gtccattttt caccaccggg ataatcgacc
     4321 ttcaggtgat gaaaattaaa ggtcatcgac aattcactgc ctgtcagtgc cgcgtatcgc
     4381 tggcaatgct caaggctggt ggaggacatt tcacctacgg tcattaaccc gcgtggcgta
     4441 aacacatcgc ggttcatctc gtgcaaaaac tcgtgtgctc gtggcccgtc ggtgtagaag
     4501 cgacgcccgt cgccatccag gtcttcaggg aaacgcgggt ctttggagat cagattcacc
     4561 acatccaggc gcaacccgtc gaccccacga tcggcccaga attcacagac ttttttcagc
     4621 tctgcgcgta ccgctggatt ctcccagttg agatccgcct gttctggtgc aaagagatgc
     4681 aaatagtact gttcgctttc cgcatgccag cgccacgcac taccgccaaa ttttgaacgc
     4741 cagttgttcg gtggcgtttc tggttcgcca tcgcgccaga tataaaactg gcggtaaggg
     4801 ctttctttgt tcagcgcctc gcgaaaccag gcatgttggg tagaggtatg gttaaacacc
     4861 atatcgagaa tgatacgaat cccgcgcgat tttgcctgcg tcaccagttc gtcaaaatcg
     4921 tccagcgtgc cgtagatggg atcaatcgcc gtatagttcg ctacgtcgta accgttatcg
     4981 acctggggag agatataaaa gggggttagc cagatggcat caacgcccag tttatgcaga
     5041 tagtccaggc gttggataac gccacgtaaa tcgccggtgc cgctacccgt ggtgtcctga
     5101 aaactctttg gataaatctg gtagataacg ccgttttgcc accagtgggg aagattagtc
     5161 attacgttat tcctgcaaat gcgagggggc gcaattgcgc cccgaagaaa attaaacaat
     5221 atccagcgtg cccaggcggt atttccgctg atagataaac gaggtgagta caatcgggat
     5281 gatgatggcg ataaccattg ccagcgcaaa tacctgccag tagctcggtt gaatcgagag
     5341 aattcccggc aggccgccta cgccgatacc attcgccata acgccgttca ggccgcatag
     5401 taatcctgcc agaccagaac caatcatcgc gcacagcatc gggaagcgat atttcaggtt
     5461 gatgccgtac attgcaggct cagtgacccc aagccaggcg gagatagcgg caggcacgga
     5521 gatctcgcgt tcattgtgct tgcggctgga aatgataatg cctatcacgg cggagccctg
     5581 agcgatattc gacagcgcaa tcagcggcca cactggcgtg ccacccatgc tttgaatcat
     5641 ctgcaaatca atagcaaggg tggtctggtg tacaccggtg atcaccagcg gggcgtacag
     5701 gaagccaaac aatgctgcgc caatcggagc aaagctgccg gtcatcaggt gacgtaccgc
     5761 aaaggcaacg ccatcgccaa tcatgcgacc aaacggacca atcagcgcat gggcgaggaa
     5821 caccgcgagg atcagcgaac agacgggcac caccaccaga tagaggtaat ccggcacgat
     5881 gcgtttaagg cgagtttcaa taacgcccag cgccagcccg gctaacagtg ccgggatcac
     5941 ctgcgcctga tagcctactt tggcgatgct gaacatgcca aagtcccaca cttccggcag
     6001 ctgctgcccg agcagataag cgttcatcag ctggggagaa accagtgtca cgccaagcac
     6061 gataccaagg atcggcgtgc cgcccatttt tttcaccgct gaccagcaaa taccgaccgg
     6121 caggtagaag aagatcgctt caccgatcaa ccacagaaaa tcgtagatcg tttgcaggga
     6181 agggtacatt tgcgccagcg tctgaccgtt gctcatgggc aaatcgccga tcacattgcg
     6241 aaaaccgagg atcaaaccgc cgctaatcaa cgcgggcagc agcgggaaga agatctccgc
     6301 gaaatgagag atcaactgct catgccattt catattctgc cgggcggctt tttttacctg
     6361 ctctttatca acctgcgcct gtccggttga cgcaatcagt gcttgatagt aatcacccac
     6421 attggtgcca atcaccacct gaaattgccc ggcattggtg aaacagcctt tcaccatggg
     6481 gagttgctca atttctttcg gtctggcatt ggccggttgg ttgaggacaa agcgcaggcg
     6541 agtaatacag tggctcaccg tcgcaatatt gccgcgcccg ccgaccagtt caatcaaccg
     6601 atcgatatcc gtttggttta ttttgctcat cataaagccc catggcagat gacatttttg
     6661 gttggctgca gaatatattg cgcggaaaaa tttaaaaacg ggaacgttcc cgaaacacag
     6721 cgaagatcac aatttatcgt tcaggaaacg atcaggacag ggtggcgggg atgatgattt
     6781 gttgcggttc gctgcgcccg gttacctgtg cgatcaattg gcaggccgcc tggcgtccag
     6841 cttcggcgta accaggatct acggtgacga tctccggatg aaggaatttc attaacggcg
     6901 tattaccgac gctcgccagt tgcaaggtgt cgatgcgttg ctcttgcagg tatttacttg
     6961 cgccaagtgc cagcgtgtcg gttgcgcaca gtaaggcggt agtttcaggc gtaatcactt
     7021 ttgctacgtt ctcatagcct tgcttcatag caagccctgg cagagcggca acgggatgca
     7081 gtttatgcgc tttgcagaac gccaggtagg cttcgtgacg tcgcttaccg gtcgtcacgt
     7141 cactgtgcgg cacgccgaga taactgatat tacgatgccc ctggtcatag agccgttgca
     7201 tcaggatctt gattgcccct tcgtcgtcat aacagaccga agcaaagcct tttgcgtcac
     7261 gcgccagcag aaccagcgat gactgccagt gggctaacat ttcttctgtt atgccggtaa
     7321 aaccgaacag cactacgccg tcgatattac gccgttttag gatccccaaa tgttcggcaa
     7381 ctaattgcgg ggaaaactga ctttccatca tgattgggtc gtaaccttgt tcatagaacg
     7441 ctggcagcat ggtttgaacg gcgagatttt ctgacaacga atccaggcgg gtaacaatga
     7501 tggcgaccac tttatcgctt tgcccacgca tagcgcgcgc agagcgggaa ggggaaaatc
     7561 catgctgatt catcactgct tccacgcgct cgcgcgtgcg ctggctcacg ccgctttcgt
     7621 tattcagcac ccgggaaacc gtagatttcc ccacgccgct taagcgcgcg atgtctttga
     7681 tggtcagccg attttgcatc ctgttgtccc gtaacgtgtt gtttaattat ttgaggctaa
     7741 ctttacccgt gcattcagca atgggtaaag tctggtttat cgttggttta gttgccagca
     7801 ggtattatat cgccataaat gctacgaata ttattggatt ctccttatta tttgcggcgc
     7861 ttttttcact taccggaggt tatatggaac ctgatcccac gcctctccct cgacggagat
     7921 taaaactttt tcggtaagcc cgtcttttca cggcgttacc ggatgcgtaa ggccgtgacg
     7981 ttttaacgtc cctgctcagc tttattacct tcaggtaagg cttcgccacg cctgaagaca
     8041 tttctgtact gtttcagaca gtgcggaggg actccttatg tttaaagaaa tttttacccg
     8101 gctcattcgc catttacctt cccgtctggt tcatcgtgat ccattgcctg gcgcgcagca
     8161 gacagtgaat acggtggtcc cgccgtcctt aagtgcgcat tgcctgaaaa tggcggtgat
     8221 gcccgaagaa gaattgtgga aaacgttcga cacccatccg gaagggttaa atcaggcgga
     8281 agtggaatct gcccgcgaac aacatggtga aaataaatta cccgcacaac aaccgtcgcc
     8341 gtggtgggta catttatggg tctgctatcg caaccccttt aatattttac tcaccattct
     8401 cggcgctatt tcttacgcca cggaagattt atttgccgcg ggcgttatcg cgctaatggt
     8461 cgccatttct acgttgctga actttattca ggaagcacgt tccactaaag cggcagatgc
     8521 cctgaaagcg atggtcagca atactgcgac ggtgctgcgc gtaattaacg acaaaggcga
     8581 aaatggctgg ctggagatcc cgatcgacca gctggtgccc ggcgatatta taaaactggc
     8641 ggcgggggat atgatcccgg cagatttacg tatcttgcag gcgcgggatc tgttcgtcgc
     8701 tcaggcgtcg ttaaccggtg agtctctgcc cgtagaaaaa gcagctacca ctcgccagcc
     8761 ggagcacagc aatccgctgg agtgcgacac cctgtgtttt atgggcacca ccgtggtgag
     8821 cggcacggca caagcaatgg tgattgctac aggtgccaat acctggtttg gtcaactggc
     8881 ggggcgtgtt agtgagcagg aaagcgagcc gaatgccttt cagcaaggga tcagccgcgt
     8941 cagtatgctg ctgattcgct ttatgctggt gatggctccg gtggtgctgt taatcaatgg
     9001 ttacaccaaa ggcgactggt gggaagcggc actgtttgcg ctttcggtcg cggtaggctt
     9061 aacgccggaa atgttgccga tgattgttac ctcgacgctg gcgcgcggcg cagtaaagct
     9121 gtcgaaacag aaagtgatcg tcaaacatct ggatgctatt cagaactttg gcgcaatgga
     9181 tattctgtgc actgataaaa ctggcaccct gacacaggac aaaattgtgc tggagaatca
     9241 taccgatatc tccggtaaaa ccagcgaacg cgtgctgcat agcgcgtggt tgaacagtca
     9301 ttatcagacc ggacttaaaa acctgctcga tacagcggtg ctcgaaggta cggatgaaga
     9361 gtcagcgcgc tcgctggcca gtcgttggca aaaaatcgat gagattccgt ttgatttcga
     9421 gcgtcgccgg atgtcggtgg tagtggcaga aaataccgag caccatcagc tggtttgcaa
     9481 aggtgcattg caggaaatcc tcaatgtgtg ttcgcaggtg cgtcacaatg gcgagattgt
     9541 gccgctcgat gacatcatgc tgcgtaagat taagcgggtt actgatacgc tgaatcgtca
     9601 ggggctgcgc gtggttgcgg tggcgacgaa atacctgcca gcgcgtgaag gagattacca
     9661 gcgggcggat gaatccgacc tgatcctcga aggatatatt gctttccttg atccgccaaa
     9721 agagacaact gctccggcac tgaaggcatt aaaagcgagt ggaattaccg taaaaatcct
     9781 tactggcgac agtgagttag ttgcagcgaa agtgtgccat gaagtggggc tggacgcggg
     9841 agaggtggtc attggtagtg atattgaaac gctatctgac gacgaactgg caaatctcgc
     9901 acagcgcacc acgctgtttg ctcgtctgac gccgatgcat aaagaacgca ttgtgacctt
     9961 actgaagcgc gaagggcatg tggttggctt tatgggcgat ggtattaatg atgcgcccgc
    10021 cttacgcgct gcggatatcg gcatttctgt ggacggcgcg gtagatattg cccgtgaagc
    10081 ggctgatatc atcctgctgg aaaaaagcct gatggtgctg gaagaggggg ttattgaggg
    10141 acgtcgcact ttcgccaaca tgctgaaata catcaaaatg acggcgagct ctaacttcgg
    10201 taatgtgttc agcgtgctgg tggcgagtgc tttcttgccc ttcctgccga tgttgccgtt
    10261 acacttgctt attcagaacc tgctgtacga tgtgtcacag gtggcgatcc cgtttgataa
    10321 cgtcgacgac gagcaaattc aaaagccgca gcgttggaat ccggcggatc tggggcgctt
    10381 tatgatcttc ttcggaccga tcagctcgat cttcgatatt ctgacgtttt gcctgatgtg
    10441 gtgggtattc catgccaaca cgccggaaac gcaaacgctg ttccagtcgg gatggtttgt
    10501 ggtgggctta ctgtcgcaaa cgctgattgt gcatatgatc cgtacccgcc gtgtgccgtt
    10561 tattcagagc tgtgcatcgt ggccgttaat gatcatgacc gtgatcgtga tgattgtcgg
    10621 gatcgcattg ccgttttcac cgctggccag ttatctgcag ctgcaggcgc tgccgttaag
    10681 ctatttcccg tggctggttg cgattctggc agggtatatg acgttaaccc agttggtgaa
    10741 agggttctat agccgtcgtt acggctggca gtaaagaata aactgggcac gatagccccg
    10801 attcagattg cagacaaagt gagcgttgtt catgccggat gcggcgtaaa tgccttatcc
    10861 ggcctacaaa ttcaatcaat tgcaggaatc acgtagatac tgataagcga agcgcatcag
    10921 gcaatgatac gtttgtcacc cagcctctgg atcacatcag ttcgggcact tataaacctg
    10981 accgataatc tggctatccg tcgggacaaa actggacaac attccctgcg acgggctgct
    11041 gatgccataa atcacgttac cgcccattgc agccgcctgg ttgcgcagat cgtttgctgc
    11101 gccgcgcata gaaccgccct cttctccgtg ttgcccggaa agccagttgc tttgcttacc
    11161 tgtcgcagta ccaatcagct ggcactctgc gcctggctgc tcgtccacaa tgcgtacact
    11221 ctgaccggca gcactcaatt cgttgctgga gctacaaccc gccatcagta gcgcggcacc
    11281 gacaatccct gctaagtatt ttacgtgcat gttattcccc ataatcaatg agctggacgc
    11341 cactcgcgtc gtccgtgtct gaatcttata ctagaaagat gaccaaaaga aaaaccccca
    11401 gacatttctg cccgggggtt tgtttatttc tgcgaggtgc agggcaatta catcatgccg
    11461 cccatgccac ccatgccgcc cataccgcca gcagcgccta agtcagctgc atcatttttc
    11521 ggcaggtcgg taaccatgca ctcggtggtg atcatcaggc cagccacaga agccgcgtac
    11581 tgcagagcag aacgggttac tttggttggg tccaggatac ccatgtcgat catgttgccg
    11641 tattcttcgg ttgctgcgtt gtaaccgtag ttgccgtcgc cgcctttaac ggtgttagca
    11701 acaacagacg gttcttcgcc gcagttcagg acgatctgac gcagcggagc ttccattgca
    11761 cgcagtgcaa ctttgatacc cacgttctgg tcttcgttct gaccacgcag gtcagccagt
    11821 ttagacgcta cgcggatcag cgcaacacca ccaccagcaa ccacgccttc ttctaccgca
    11881 gcacgggtcg cgtgcagggc gtcttcaacg cgtgcttttt tctctttcat ttcaacttcg
    11941 gtagcagcac ctactttgat aactgcaacg ccgcctgcca gtttcgctac gcgttcctgc
    12001 agtttttcac ggtcgtagtc agaagttgct tcttcaatct gctgacggat ctgagcaaca
    12061 cggccctgga ttgcagcttc ttcacccacg ccatcgatga tagtggtggt gtctttgttg
    12121 atcacaacac gtttagcctg acccaggtct tccagggttg ctttttccag ctccataccg
    12181 atctcttcag agattacggt accgccagtc agggttgcga tatcctgcag catagcttta
    12241 cgacgatcgc cgaagcccgg agctttaacc gcagcaactt tcacgatgcc acgcatggtg
    12301 ttaacaacca gagttgccag cgcttcgcct tcaacatctt cagcgatgat cagcagcggt
    12361 ttgcctgctt tggcaacagc ttccagaacc ggcagcattt cgcggatgtt agagattttc
    12421 ttgtcagcca gcaggatgaa cgggctttcc agttctactg cgccagtttc cggcttgttg
    12481 atgaagtaag gagacaggta gccacggtcg aactgcatac cttcaaccac gtccagttcg
    12541 tcctgcagac cggtaccgtc ttcaacggtg ataacgcctt ctttaccgac tttgtccatc
    12601 gcttcagcga tcagtttacc tacggtttcg tcggagttag cggagatggt accaacctga
    12661 gcaatcgctt tagagtcaga gcacggtacg gacagcgctt tcagttcttc aactgcagcg
    12721 gtaacggctt tgtcgatacc acgtttcagg tccatcgggt tcatgcccgc agcaacagct
    12781 ttcagaccct cagtgatgat agcctgagcc agtacagttg cagtggtggt accgtcgcct
    12841 gcagcgtcgt tcgctttaga ggcaacttct ttcaccatct gcgcacccat attttcgaac
    12901 ttgtcttcca gttcgatttc acgagcaacg gaaacaccat ctttggtgat ggtcggtgca
    12961 ccgaaagatt tatccagaac tacgttacgg cctttcggac cgagggtaac tttcactgca
    13021 tctgccagta cgtttacgcc gcgcagcatt ttcacacgag cgtcgttacc gaattttacg
    13081 tctttagctg ccattatctt tattccttaa attcgtatgt tcagtgtcgt gcgaggatta
    13141 cgcttcaaca attgccagaa tgtcgctttc ggacatgatc aacacttctt cattgtcgat
    13201 cttctcagat ttcacaccgt agccatcgtt gaaaataacg atgtcgccaa ctttcacatc
    13261 cagcggcttc acttcgccat tttcaaggat acggccattg ccgacagcca gcacttcgcc
    13321 gcgggtggat ttagccgctg cagagccggt cagaacgatg ccgccagcag atttagtttc
    13381 aacttcttta cgcttgacga tcacgcgatc atgcaatgga cgaatattca ttgataactc
    13441 tcctttgaga aagtccgtat ctgttatggg tgacgccgga gcttacgtgg tttcccggct
    13501 ggtgaccaga gaaatgggga tgaggattcg ccccttcaag ggggaaaaag aaaaaaaatt
    13561 ctgatcagca caaaatcggg tgaaaaccct gattcacctc acatttcatc gcaatttctt
    13621 catcgccgtc ataagcgaat ctgattgtgc taccatcgaa aatctacgca gttgcccaaa
    13681 atttgggcgc aatcggatat caacccatga gtggactcaa acaagaactg gggctggccc
    13741 aaggcattgg cctgctatcg acgtcattat taggcactgg cgtgtttgcc gttcctgcgt
    13801 tagctgcgct ggtagcgggc aataacagcc tgtgggcgtg gcccgttttg attatcttag
    13861 tgttcccgat tgcgattgtg tttgcgattc tgggtcgcca ctatcccagc gcaggcggtg
    13921 tcgcgcactt cgtcggtatg gcgtttggtt cgcgacttga gcgagtcacc ggctggttgt
    13981 ttttatcggt cattcccgtg ggtttgcctg ccgcgctaca aattgccgcc gggttcggcc
    14041 aggcaatgtt tggctggcat agctggcaac tgttgttggc agaactcggt acgctggcgc
    14101 tggtgtggta tatcggtact cgcggtgcca gttccagtgc taatctacaa accgttattg
    14161 ccggacttat cgtcgcgctg attgtcgcta tctggtgggc gggcgatatc aaacctgcga
    14221 atatcccctt cccggcacct ggtaatatcg aacttaccgg gttatttgct gcgttatcag
    14281 tgatgttctg gtgttttgtc ggtctggaag catttgccca tcttgcctcg gaatttaaaa
    14341 atccagagcg tgattttcct cgcgcattga tgattggtct gctgctggca ggattagtct
    14401 actggggctg tacggtagtc gtcttacact tcgacgccta tggtgaaaaa atggcggcgg
    14461 cagcatcgct tccaaaaatt gtagtgcagt tgttcggtgt aggagcgtta tggattgcct
    14521 gcgtgattgg ctatctggcc tgctttgcca gtctcaacat ttatatacag agcttcgccc
    14581 gcctggtctg gtcgcaggcg taacataatc ctgaccacta cctggcacgc ctctcttctc
    14641 gccatatccc gaataatgcc ctcaatgcgg tgctcggctg ctgtgtggtg agcacgttgg
    14701 tgattcatgc tttagagatc aatctggacg ctcttattat ttatgccaat ggcatcttta
    14761 ttatgattta tctgttatgc atgctggcag gctgtaaatt attgcaagga cgttatcgac
    14821 tactggcggt ggttggcggg ctgttatgcg ttctgttact ggcaatggtc ggctggaaaa
    14881 gtctctatgc gctgatcatg ctggcggggt tatggctgtt gctgccaaaa cgaaaaacgc
    14941 cggaaaatgg cataaccaca taatccggcg tttcgacatt aatcctggcg atcgtcttta
    15001 tgatcaaggc ggtcgcgctc atcatccttt cgctggtact caccatcaaa agtattaccg
    15061 ccaccggtcc cggcgctaaa accgccgcct ggcatacgag aaaagcgcaa atgcggcatc
    15121 aacttcactg tcagatgctt ttgcaccggc ggtaataaaa gcagaagacc gaggaagtcg
    15181 gtgaaaaagc ccggtaataa aagcagcaaa ccggcaatga tcagcgaaac acttttaatc
    15241 atctccgccg ctgggttttc acccgccgcc attttttgct gcatcagcac gaaattctta
    15301 aagccctggt tacgtaccag tgacatcccg ataactgacg tgaatataac cagcacgagg
    15361 gtcagcaata cccccaatac atgggcaacc tgaataaaga ttgaaatctc aatatagaca
    15421 taaaggaaaa tggcaataaa aggtaaccag cgcaaaggtt tctcctgtaa tagcagccgg
    15481 taaaacccgg ctacctgaat gggttgcgaa tcgcgttcag cttatattgt ggtcattagc
    15541 aaaatttcaa gatgtttgcg caactatttt tggtagtaat cccaaagcgg tgatctattt
    15601 cacaaattaa taattaaggg gtaaaaaccg acacttaaag tgatccagat tacggtagaa
    15661 atcctcaagc agcatatgat ctcgggtatt cggtcgatgc aggggataat cgtcggtcga
    15721 aaaacattcg aaaccacata tattctgtgt gtttaaagca aatcattggc agcttgaaaa
    15781 agaaggttca catgtcaaac aacattcgta tcgaagaaga tctgttgggt accagggaag
    15841 ttccagctga tgcctactat ggtgttcaca ctctgagagc gattgaaaac ttctatatca
    15901 gcaacaacaa aatcagtgat attcctgaat ttgttcgcgg tatggtaatg gttaaaaaag
    15961 ccgcagctat ggcaaacaaa gagctgcaaa ccattcctaa aagtgtagcg aatgccatca
    16021 ttgccgcatg tgatgaagtc ctgaacaacg gaaaatgcat ggatcagttc ccggtagacg
    16081 tctaccaggg cggcgcaggt acttccgtaa acatgaacac caacgaagtg ctggccaata
    16141 tcggtctgga actgatgggt caccagaaag gtgaatatca gtacctgaac ccgaacgacc
    16201 atgttaacaa atgtcagtcc actaacgacg cctacccgac cggtttccgt atcgcagttt
    16261 actcttccct gattaagctg gtagatgcga ttaaccaact gcgtgaaggc tttgaacgta
    16321 aagctgtcga attccaggac atcctgaaaa tgggtcgtac ccagctgcag gacgcagtac
    16381 cgatgaccct cggtcaggaa ttccgcgctt tcagcatcct gctgaaagaa gaagtgaaaa
    16441 atatccaacg taccgctgaa ctgctgctgg aagttaacct tggcgcaaca gcaatcggta
    16501 ctggtctgaa cacgccgaaa gagtactctc cgctggcagt gaaaaaactg gctgaagtca
    16561 ctggcttccc atgcgtaccg gctgaagacc tgatcgaagc gacctctgac tgcggcgctt
    16621 atgttatggt tcacggcgcg ctgaaacgcc tggctgtgaa gatgtccaaa atctgtaacg
    16681 acctgcgctt gctctcttct ggtccacgtg ccggcctgaa cgagatcaac ctgccggaac
    16741 tgcaggcggg ctcttccatc atgccagcta aagtaaaccc ggttgttccg gaagtggtta
    16801 accaggtatg cttcaaagtc atcggtaacg acaccactgt taccatggca gcagaagcag
    16861 gtcagctgca gttgaacgtt atggagccgg tcattggcca ggccatgttc gaatccgttc
    16921 acattctgac caacgcttgc tacaacctgc tggaaaaatg cattaacggc atcactgcta
    16981 acaaagaagt gtgcgaaggt tacgtttaca actctatcgg tatcgttact tacctgaacc
    17041 cgttcatcgg tcaccacaac ggtgacatcg tgggtaaaat ctgtgccgaa accggtaaga
    17101 gtgtacgtga agtcgttctg gaacgcggtc tgttgactga agcggaactt gacgatattt
    17161 tctccgtaca gaatctgatg cacccggctt acaaagcaaa acgctatact gatgaaagcg
    17221 aacagtaatc gtacagggta gtacaaataa agaaggcacg tcagatgacg tgcctttttt
    17281 cttgtgagca gtaacttaaa aataacaacc taatatcaac ttgttaaaaa acaaggaagg
    17341 ctaatatgct agttgtagaa ctcatcatag ttttgctggc gatcttcttg ggcgccagat
    17401 tggggggaat aggtattggt tttgcaggcg gattgggggt gctggttctt gccgctattg
    17461 gcgttaaacc cggtaacatc ccgttcgatg tcatttccat tatcatggcg gttatcgccg
    17521 ctatttctgc catgcaggtt gctggcggtc tggactatct ggttcatcag acagaaaagc
    17581 tgctgcgccg taacccgaaa tacatcacga tcctcgcacc gatcgtgacc tatttcctga
    17641 ctatctttgc tggtactggc aacatatctc tggcgacact gccagttatc gctgaagttg
    17701 cgaaggaaca aggcgtcaaa ccttgccgtc cgctgtctac tgcagtggta tccgcgcaga
    17761 ttgcgatcac cgcatcgcca atctcagcgg cagtggttta catgtcttcc gtgatggaag
    17821 gtcatggcat cagctacctc catctgctct ccgtggtcat cccgtccacc ctgctggcgg
    17881 ttctggtgat gtcctttctg gtcactatgc tgttcaactc caaactctct gacgatccga
    17941 tttatcgcaa gcgtctggaa gagggcctgg ttgaactgcg cggtgaaaag cagattgaaa
    18001 tcaaatccgg tgcaaaaacg tccgtctggc tgttcctgct gggcgtagtt ggcgtggtta
    18061 tctatgcaat catcaacagc ccaagcatgg gtctggttga aaaaccgctg atgaacacca
    18121 ccaacgcaat cctgatcatc atgctcagcg ttgcaactct gaccaccgtt atctgtaaag
    18181 tcgataccga caacatcctc aactccagca ccttcaaagc aggtatgagc gcctgtattt
    18241 gtatcctggg tgttgcgtgg ctgggcgata ctttcgtttc caacaacatc gactggatca
    18301 aagataccgc tggtgaagtg attcagggtc atccgtggct gctggccgtc atcttcttct
    18361 ttgcttctgc tctgctgtac tctcaggctg caaccgcaaa agcactgatg ccgatggccc
    18421 tggcactgaa cgtttcaccg ctgaccgctg ttgcttcttt cgctgcggtg tctggtctgt
    18481 tcattctgcc gacctacccg acgctggttg ctgcggtaca gatggatgac acgggtacaa
    18541 cccgtatcgg taaattcgtc ttcaaccatc cgttcttcat cccgggtact ctgggtgttg
    18601 ccctggccgt ttgcttcggc ttcgtgctgg gtagcttcat gctgtaatga ccaatcgcgg
    18661 ggcgttcacg ccccgctttc tttcccgccg actaacatcc ttcccccgtc cgttgtatag
    18721 tgacctctct cttgcggttc catctgttct tgcgaggtgt ttatgcttga tgaagaaagt
    18781 tcgaataccg cgtctgtcgt ggtgctatgt accgcaccgg atgaagcgac agcccaggat
    18841 ttagccgcca aagtgctggc ggaaaaactg gcggcctgcg cgaccttgat ccccggcgct
    18901 acctctctct attactggga aggtaagctg gagcaagaat acgaagtgca gatgatttta
    18961 aaaactaccg tatctcacca gcaggcactg ctggaatgcc tgaagtctca tcatccatat
    19021 caaaccccgg aacttctggt tttacctgtt acacacggag acacagatta cctctcatgg
    19081 ctcaacgcat ctttacgctg atcctgctac tttgcagcac ttccgttttt gccggattat
    19141 tcgacgcgcc gggacgttca cattttgtcc ccgcggatca agcctttgct tttgattttc
    19201 agcaaaacca acatgacctg aatctgacct ggcagatcaa agacggttac tacctctacc
    19261 gtaaacagat ccgcattacg ccggaacacg cgaaaattgc cgacgtgcag ctgccgcaag
    19321 gcgtctggca tgaagatgag ttttacggca aaagcgagat ttaccgcgat cggctgacgc
    19381 ttcccgtaac catcaaccag gcgagtgcgg gagcgacgtt aactgtcacc taccagggct
    19441 gtgctgatgc cggtttctgt tatccgccag aaaccaaaac cgttccgtta agcgaagtgg
    19501 tagccaacaa cgcagcgtca cagcctgtgt ctgttccgca gcaagagcag cacaccgcgc
    19561 aattgccctt ttccgcgctc tgggcgttgt tgatcggtat tggtatcgcc tttacgccat
    19621 gcgtgctgcc aatgtaccca ctgatttctg gcatcgtgct gggtggtaaa cagcggctct
    19681 ccactgccag agcattgttg ctgaccttta tttatgtgca ggggatggca ctgacttaca
    19741 cggcgctggg tctggtagtt gccgccgcag ggttacagtt ccaggcggcg ctacagcacc
    19801 catacgtgct cattggcctc gccatcgtct ttaccttgct ggcgatgtca atgtttggct
    19861 tgtttactct acaactcccc tcttcgctgc aaacacgtct cacgttgatg agcaatcgcc
    19921 aacagggcgg ctcacctggc ggtgtgtttg ttatgggggc gattgccgga ctgatctgtt
    19981 caccatgcac caccgcaccg cttagcgcga ttctgctgta tatcgcccaa agcgggaaca
    20041 tgtggctggg cggcggcacg ctttatctct atgcgttggg catgggcctg ccgctgatgc
    20101 taattaccgt ctttggtaac cgcttgctgc cgaaaagcgg cccgtggatg gaacaagtca
    20161 aaaccgcgtt tggttttgtg atcctcgcac tgccggtctt cctgctggag cgagtgattg
    20221 gtgatgtatg gggattacgc ttgtggtcgg cgctgggtgt cgcattcttt ggctgggcct
    20281 ttatcaccag cctacaggct aaacgcggct ggatgcgcgt ggtgcaaata atcctgctgg
    20341 cagcggcatt ggttagcgtg cgcccacttc aggattgggc atttggtgcg acgcataccg
    20401 cgcaaactca gacgcatctc aactttacac aaatcaaaac ggtagatgag ttaaatcagg
    20461 cgctcgttga agtcaaaggc aaaccggtga tgttagatct ttatgccgac tggtgcgtcg
    20521 cctgtaaaga gtttgagaaa tacaccttca gcgacccgca ggtgcaaaaa gcgttagcag
    20581 acacggtctt acttcaggcc aacgtcacgg ccaacgacgc acaagatgtg gcactgttaa
    20641 agcatcttaa tgtccttggc ctaccgacaa ttctcttttt tgacggacaa ggccaggagc
    20701 atccacaagc acgcgtcacg ggctttatgg atgctgaaac cttcagcgca catttgcgcg
    20761 atcgccaacc gtgaacgaca cttgcagtgg gaaagacgga ggaaaatagc gtgcaacgtg
    20821 aagatgtact gggagaagcc ctgaaattat tagaattaca agggattgcc aacaccacgc
    20881 tggagatggt tgctgaacgt gtggattatc cactggacga gctacgccgc ttctggccag
    20941 acaaagaggc aatcctctac gatgcgctgc gttatcttag ccaacagata gatgtctggc
    21001 gtcgtcagct gatgctggac gaaacgcaaa ccgccgaaca aaagctgctg gcacgttatc
    21061 aggcgttatc ggagtgcgtt aaaaacaacc gctatccggg ctgtctattt atcgctgcct
    21121 gtacgtttta tcccgatcct ggccacccta ttcatcaact ggccgatcag caaaaaagcg
    21181 cggcctacga tttcacccac gaactgttaa ccacgctgga agttgacgat ccggcgatgg
    21241 tagcaaagca gatggaactg gtgctggaag gctgtttaag ccgaatgctg gtgaatcgta
    21301 gccaggcgga tgtcgacact gcacatcggc tggcggaaga tatcctgcgc ttcgcccgct
    21361 gccgccaggg tggtgcattg acctgacaga aacacagaaa agaagcgatt tgccgcaatc
    21421 ttaagcagtt gaatcgcttt tactgaaatt aggttgacga gatgtgcaga ttacggttta
    21481 atgcgccccg ttgcccggat agctcagtcg gtagagcagg ggattgaaaa tccccgtgtc
    21541 cttggttcga ttccgagtcc gggcaccaaa ttcatatcaa cggacctcca cggaggtccg
    21601 tttttcgttt cagaacacca tgatttaagt gttcttcc
//
LOCUS       DAZGLJ010000060        21541 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000060 DAZGLJ010000000
VERSION     DAZGLJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.060"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..322
                     /locus_tag="ACHK49_002041"
     CDS             <1..322
                     /locus_tag="ACHK49_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9262691.1"
                     /translation="TLEQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSL
                     TNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPV
                     SLVG"
     gene            complement(365..1516)
                     /gene="ydiO"
                     /locus_tag="ACHK49_002042"
     CDS             complement(365..1516)
                     /gene="ydiO"
                     /locus_tag="ACHK49_002042"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO9262692.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1530..3124)
                     /gene="ydiF"
                     /locus_tag="ACHK49_002043"
                     /pseudo
     CDS             complement(1530..3124)
                     /gene="ydiF"
                     /locus_tag="ACHK49_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
     gene            complement(3268..4026)
                     /gene="aroD"
                     /locus_tag="ACHK49_002044"
     CDS             complement(3268..4026)
                     /gene="aroD"
                     /locus_tag="ACHK49_002044"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO9262693.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4057..4923)
                     /gene="ydiB"
                     /locus_tag="ACHK49_002045"
     CDS             complement(4057..4923)
                     /gene="ydiB"
                     /locus_tag="ACHK49_002045"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO9262694.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4935..6200)
                     /gene="ydiN"
                     /locus_tag="ACHK49_002046"
     CDS             complement(4935..6200)
                     /gene="ydiN"
                     /locus_tag="ACHK49_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9262695.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(6427..7641)
                     /gene="ydiM"
                     /locus_tag="ACHK49_002047"
     CDS             complement(6427..7641)
                     /gene="ydiM"
                     /locus_tag="ACHK49_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9262696.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGHRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVDSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(7741..8097)
                     /gene="ydiL"
                     /locus_tag="ACHK49_002048"
     CDS             complement(7741..8097)
                     /gene="ydiL"
                     /locus_tag="ACHK49_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO9262697.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8233..8340)
                     /gene="rprA"
                     /locus_tag="ACHK49_002049"
     ncRNA           complement(8233..8340)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHK49_002049"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8526..9638)
                     /gene="ydiK"
                     /locus_tag="ACHK49_002050"
     CDS             complement(8526..9638)
                     /gene="ydiK"
                     /locus_tag="ACHK49_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO9262698.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            10027..13083
                     /gene="ydiJ"
                     /locus_tag="ACHK49_002051"
     CDS             10027..13083
                     /gene="ydiJ"
                     /locus_tag="ACHK49_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO9262699.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13080..13490
                     /gene="menI"
                     /locus_tag="ACHK49_002052"
     CDS             13080..13490
                     /gene="menI"
                     /locus_tag="ACHK49_002052"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO9262700.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13590..13778
                     /gene="ydiH"
                     /locus_tag="ACHK49_002053"
     CDS             13590..13778
                     /gene="ydiH"
                     /locus_tag="ACHK49_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO9262701.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14326..14694
                     /gene="sufA"
                     /locus_tag="ACHK49_002054"
     CDS             14326..14694
                     /gene="sufA"
                     /locus_tag="ACHK49_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO9262702.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14703..16190
                     /gene="sufB"
                     /locus_tag="ACHK49_002055"
     CDS             14703..16190
                     /gene="sufB"
                     /locus_tag="ACHK49_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO9262703.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16200..16946
                     /gene="sufC"
                     /locus_tag="ACHK49_002056"
     CDS             16200..16946
                     /gene="sufC"
                     /locus_tag="ACHK49_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO9262704.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYRGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16921..18192
                     /gene="sufD"
                     /locus_tag="ACHK49_002057"
     CDS             16921..18192
                     /gene="sufD"
                     /locus_tag="ACHK49_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO9262705.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18189..19409
                     /gene="sufS"
                     /locus_tag="ACHK49_002058"
     CDS             18189..19409
                     /gene="sufS"
                     /locus_tag="ACHK49_002058"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO9262706.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19422..19838
                     /gene="sufE"
                     /locus_tag="ACHK49_002059"
     CDS             19422..19838
                     /gene="sufE"
                     /locus_tag="ACHK49_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO9262707.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            19987..20991
                     /gene="ldtE"
                     /locus_tag="ACHK49_002060"
     CDS             19987..20991
                     /gene="ldtE"
                     /locus_tag="ACHK49_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO9262708.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21054..21290)
                     /gene="lpp"
                     /locus_tag="ACHK49_002061"
     CDS             complement(21054..21290)
                     /gene="lpp"
                     /locus_tag="ACHK49_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO9262709.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         5599 a         5236 c         5445 g         5261 t
ORIGIN      
        1 cactttagaa cagttatcga aaaaatttcg tgccagcgtc agttatattt gccatgaatt
       61 taccaaagag tatcgtattt cccctattaa ctatgttatt caacggcgta tgacggaagc
      121 aaaatggtca ctcactaata ctgaattatc acaggcagag atctcctggc gtgtgggtta
      181 tgaaaatgtc gatcactttg ccaaactgtt tttgcgccat gtcggttgtt cgcccagcga
      241 ttaccgtaga caatttaaaa actgttttgc ggaacaagaa atcctatctg aatttcctca
      301 accggtaagt cttgttggat aaatctggcg gcgcagtttg ttaagaagct gcgccgcctg
      361 cagattattt gttctgatag tctttcagga tctgccgacc tgctacgtaa atcataattt
      421 cgtctgtacc gccgccgata cgttcacaac ggacatcacg ccagaagcgg gagacgcgcg
      481 cttcatcggt atagcccaga ccgcccatga tttgaatcgc atcatcaatg acttccattg
      541 cggtacgtgc gcaatacagt tttgccagcg ccgcgctggt gcgcagtgac tgatgctgat
      601 cggcttgcca tgccactttc agcaccatgt tgcgcatgtt gtcgatctta atcgccatca
      661 gcgccagttt ttcctggatc atctggttat gaccaatggg cttaccaaaa gcgatacgtt
      721 ggttggcata gcgggcggca tcttcaaagg cgcattcggc aaagccggtg ctgcgcgcgg
      781 cgttgatcag gcgctccatc tcaaagttgt acatcacatt gaggaaaccc attccttctt
      841 cgcccaccat gtcgctctct tcaacttcca cattgtcgag atagacttcg caggtgctga
      901 gcatatgcca gccgatttta tgcagcgggt taatcttaat gccgggctta ctggagtcga
      961 cccaccacag ggtgaaggct tttttgggat ctttcggttg cggatcgcgc gccagcacca
     1021 gcatatacgg gtactctttc gcaccggtaa taaaggtttt ttgtccgttg atgtagacct
     1081 tgccgttttt acgcgtgtaa gtggtagtgg cactgttgtt atctgagcca gcacctggtt
     1141 ccgtcaacgc cagggcatag gcgggatcac ccgtttccag ggtgctctct gccgttttac
     1201 gtagctgctc tgcagaaccg aaacggcgca tactgtgaat acattgaccg ttggtaatta
     1261 aaaatgccgg agcaccgcat tttgacactt ccatcagcgc cagcatttgg gtgacgtaat
     1321 ccgcagggat accaccaaat tcttccggca cgccaagcat ggaaataccg ttatccgcca
     1381 gcgcccgcat aaactcacgc ggatatgtcc cgttttgatc gcaggtgcgg aaatactctt
     1441 ccggaaagtt agtcgtaatc agttcgcgaa tactggccag cagcagttct tgttcttcag
     1501 ttaaagaaaa atccatcatt atactcctgt taatgagccg cttcaggcag gacaaaaccc
     1561 atcgccgcat cgataaataa tctttcgtcc atcagtttga gttctggcga aatcactggg
     1621 gtgaagtcca ttttgtcgag aatatctttt tgtaaatcga cgccaggggc gatttcgatt
     1681 aaatgcaggc cgtcttcttt cagcgtgaat acagcacgtt cggtgatgta acgaacatcc
     1741 agtccccgtt cgagcgcgat ttttccgcta aaagtaattt ccggtagttc ccgaataaat
     1801 ttatttaccc gtccttcctg gacgatatgc aatttgccat cggcaatttc tgtttttaaa
     1861 ctgcccgcag ttaacgtgcc gcagaaaata attttcttcg aggtggcact gatatcaata
     1921 aatccaccgg tacccatgat tttgccattg aatttatgca cgccgacgtt accgtgctgg
     1981 tcgacttcag caaaactcaa ataacaaaca tccagaccac caccgtgata aaaatcaaac
     2041 tgggacgtca tatccagaat ggcgcgggta ttcacgttcg cgccaaaggc gataccctgt
     2101 gaagtaattc cgccaatcgg acctgtttct accgtcagaa taaagtcatc agcacaacct
     2161 tcttcccggg cgaccaggcc aataccgtca gcaataccga cgccgacatt ccccaccgcg
     2221 cctttacgca tttcgaataa tgcacgccgc gcaactaatt tacgttggtt taggggcagg
     2281 ctaagtttgt gctgtcatca agggtgaagt caccagaaat aaaacggtta accggcgcac
     2341 cgccatacag ttggctttga tccggatcga ccaccacaat atccaccaga taacccggaa
     2401 tacggacaga tttaggatgc agcgtggctt tcttaaccat tttctgcacc tgcatcatca
     2461 caataccgcc gttattgtgc accgcctggg caataaccaa tgcgtcgaga tacatcacct
     2521 catcttcaaa agtggcgtag ccttcactgt cgcaggtggt agcgcgaatg aaggcgatat
     2581 ctggcgcaat cgctttgtaa tagagatatt ctttgttatc aaactcgacc agtttaatca
     2641 ggtcttcttt cgtgacttca ttcagtttgc cgccttgctg gcgtggatcg acaaatgtcc
     2701 cgatgccaat atcactaata ataccaggct ggtgggctgc ggcggcgcgt aaggtttgtg
     2761 taagtacacc ttgtggatag ttataagcaa taattttatt ttgttctgcg agttcagaaa
     2821 tacgcggcga ttgtccccag tgaccacata atgcccattt caccagacct tcttgcgcca
     2881 gaggactaat accacggtcg gcgcgatcgc caagccctgt cggactaata atcgataaat
     2941 tacgtggcgt ttgagtctgt ttatatttat cagcaagagc tgtaattaac gtggtggctt
     3001 ccagaatacc gccgccagcg cctaacacac aaagtgttgc ttcgtcggga atataattca
     3061 ccgcttcctg tgccgacagg accggcactc gtccattaat acgaggtggt tttacaggtt
     3121 tcattgttaa ttcctgattc agatgtgatt ttccctacgc cagatggaga attagcgcac
     3181 agagactcac gaaatatttt tattaaatac ggttaaatat attttttaag ttcggcgggg
     3241 agggtgttcc cgccgaaata ttattgctta tgcctggtgt aaaatagtta ataccgtgcg
     3301 taaatcattt accgagattt gccctggtgc agaggctttt tttaccgcac caaaagttgc
     3361 cgccgagcca aatacttcac cagccagacg agaaattacg ccagtttttg ccatcgacat
     3421 cgtgatgatt ggacgatcgg catactgctc ctgcatctcc agggtcgcgg taagcaacgt
     3481 cagcacatcg ctggtacttt gcggcatcag cgcaatctta ggaatatcgg cgtcgaagga
     3541 ctgcattttg cgcagacggg caatgatttc ttcggcttcc ggcgttttat ggaagtcatg
     3601 gttggacatg acaactttca catcatgcgc gtgggcgtag gcgacggttt ctttaacctg
     3661 atcatcgccg gtaaataact ccagatcgat catatcaacc aggccgctgt cgatggctgc
     3721 acgattgaga gcaatataag cctcggtgga aatcgcctgc tcgccgcctt ctttggcact
     3781 gcggaaggta aacagcagcg gtttttctgg catggtttca cggagaattt ttgccgccgc
     3841 catgacagac tccacattgg agaggtcggc aaagtggtcc acacgccatt ccagaatatc
     3901 aaagtccgct tcacgatagg cgagagcttc ggatttcacg cgggcgatat ctttcgccat
     3961 cagcgagacg atgattttag gtgcgcccgt accaatgacg agatctttta cagttacggt
     4021 tttcattttt taccctttct gcacgcggtc agcctgtcag gcaccgaacc ccatgacctg
     4081 tttaacatat tccagaggga aatctttgcc agtccataac gtgaactgtt cagccccttg
     4141 ccacaacaac atgccgtatc catcaatcgt tttgcaacca gcttgttgcg cctgctgcaa
     4201 taacttcgtc atatgcgggt tatacacgca ttcagtgacc agcagtcccg gatgtaacag
     4261 actgatatca ttaactaatg attcattctc aaggggtttc atacccactt ttgtgccatt
     4321 ggttaaaatg tcggcggaag ccagggcttc agcaaaggct tgctgatcgg cgagatcggt
     4381 aaccgtgacg acacaatcgg tgttttcgtt aacccgctgc gcgaaggcga gggctttatc
     4441 gaagaactca tcccgacggt taaagagttt aatttctttt aaaccttcaa ttgccccctg
     4501 cgcgccaatc gccgttgagg cacctccggc ccctaacagc accattgttt tgcctttgat
     4561 atcaaaaccg ctctctttaa tggcgcgaat atggcccgtg ccgtcggtgt tatagccgcg
     4621 caggtagcca tcatcattaa cgatggtgtt gatggcaccc accagtttgg ctgctggcgt
     4681 taattcatca acatattcac acgccagttg tttgttcggc attgatatac cagttccgcg
     4741 cattttgagg gcttttaatc cttcaattgc tccgggaaaa ctatcgttat ccacttcaaa
     4801 ggccatatag gtaaatggca atcccgcttt ttctaaggct ttattctgca tttcaggcga
     4861 taaactgtgg cggataggat aggccatcag cccaatcaat tcgtattttg cggtaacatc
     4921 cataattaac tcctttaacc tctatgctta attgtgttta accgggtgct aaatttacgc
     4981 tcgccaaacc gcacatcatt ctcaggaata ataaaaacgc ggtaatagcg gataaaaaca
     5041 ataattgcgg taatcagggc cagcagcgcg aaagtaaaat cgagaacaat gatatattga
     5101 aggccgatgt tcgacagata accggtaatc agcggaataa caaagttagc cagtccaccc
     5161 atcatcatat aaatactggt gactttggct ttgctttttg ggaaaaactc tgacatgacc
     5221 gaaacaccga gctgtaaaat gccgccagct gctgagaaac cgataacaaa ggcgccggca
     5281 ttacacacca gtggagaagg gtacaggtag ataatggctg ctgttattgt tgccagtgca
     5341 gagttaaata cattagccca gatgggccgg accatttttt tcagtagtgc ggcaaaaata
     5401 aagacacaga ccaacgagcc catactgtaa taagagatgg tttttaatgc ctcagcttct
     5461 gacataccag caaaagccat cgcatatttg ggcatccaca ccacaatcac ataaaaggtc
     5521 gagaatgcgg ctacaccgaa cagtaccgat gaaacacctt ccagccagac taacggtttg
     5581 ctgttcattt gcggtaattc attagctacg ctggcgtcca ccaactggct ggggaatttg
     5641 cttttcaaca gcatcagcgt gatcagtaca aacagaatac ccggaataat cagcccatag
     5701 ccgtaccaga tattattgag caacatatag ctcaccagca ttgggtagaa catttgccca
     5761 aatgacacca tcgctttaac cagtatgacc gccgaaccag aggctttcgg aaagcattcc
     5821 atgagcgcgg ggtagccacc cgtatccagc gctgagttag cgatacctac gcacactgcc
     5881 agaccgtagg cgagagttaa attcgggcaa gcgggaatac caaagaagaa tagcagatac
     5941 attattactg ccattaatat caccgcccga cgaccaaact tatcggagat cacaccgaag
     6001 aataaaatac tgatcaatcg ccccaaacca ataccggaaa ttaagtaggc aatgcccgcg
     6061 ttgtcagtgg aaaacttttc cgccagagat gacatgtttt gggcaagcgt aataacacta
     6121 atgccgtgca ggaagtagct gaagtaaata caaagaacag ccaggataaa tggcgtgctg
     6181 aaagccttat tttgagacat tttcacagct ccttgctgaa agataatttc gttaccgtac
     6241 gtattgttct tcgacggttt acataattac tatactgaag atgattttcc ctgggtacat
     6301 ggcataaagt ttatgtttcc aggaatattg catgtcatag tttgtttttg atcgcgattt
     6361 tccttagtta gcgcagaaag agttatttat ccgtaagatc ttgcatgacc tgaaaagaat
     6421 attgcattac ccacccggag cgacattttc ctttagcgag tgatgccgcg tttttttgcg
     6481 gctgcgtaag ccgataaaca gtgccagtaa aaaaccgatg gcagcgatgg cggtatcgaa
     6541 ccacataata tcggcaatac ttctttggga cagatgagcc gtaatcaacg gaatagtaaa
     6601 ggtcgcaata ctgcccgcac tgtaatagat ccctgtagct ttacctttag cgtaagggaa
     6661 acgttcagcc attaacgtca ggccaatttg cacaacacct ccagcagagg taaaaccaat
     6721 gacaaaagca aatattatca ccacataaaa tgtgggatgc aggcagacgg taaacagagc
     6781 aataaatgag ataaaggtgt acagcatcag taatgttgtt gggcgaacgg tattacgaat
     6841 gagtggagcg gtaataaata cacaaagcag cgaatccacg gtgtagatac tgagtagttt
     6901 aatcgacata gtgtatgaca tgcctgcaac aaattgtccg tactgtgcca gccactggct
     6961 aaccagataa aacgttgcca ttgagatata gccatataag gtataactgg ctaaatcgat
     7021 aattgaacag cgatgttccg tagagctggt ggttttcttt atgacaggta agcgatgacc
     7081 cggatggggt gggaacgtac aacggtataa aaacagagcg ttaataaaca taatgcctgc
     7141 agcaatcata aaggaccaac cgaaccacag ttcagcccac actaacaggc taatgattag
     7201 cggtaataaa aattgtccgc tggaaacaaa tgctttaatt aaaatattgg ctgtcccagg
     7261 tgagcgtgga aaagcttcca tcaaactggg ataagtgcct gcatcgagaa aactgtttgc
     7321 cattcctgcc agaaagccaa aaacataagc gataatgatg ttattggtgt gcaggatgcc
     7381 aaaaaagaag gccatatagc agcacatccc gagcatgata aaagggcggc gaccaaagcg
     7441 atcggataat aatcctgcaa aaagcaagac acttaatcga ccaatgccca gcgatgagat
     7501 aactatcgag acacccgcgg cattagtctg ccaaagtgtc tccagcgagg ccatattcag
     7561 gctcatcaaa atgacgccca taccatgcac taggtaatta aaatacaacc caagtgcggt
     7621 agggtaatag ggatttttca taggttctac ttagcaaagg tgctttaaaa agtgtagcga
     7681 tgttgtgtca gtatccgttc attattgttg taacaacgac aattcctttt tgtggagaaa
     7741 ttaacaaagc cgctccagat cgtgtgcata taattcagcg gctaccgatt gatagatctt
     7801 ccagtcaata aaattgccat cagggtagac ttgttgaaac gcggttaagt cggggaaaaa
     7861 acgcatggtg ttgttgccga tgcggttatt tattttctct atgatagcat gaagatgttt
     7921 ttttcgttgc tgacgcatag ccaacagttg ctcgactaca cgatcaggaa tggcgcattt
     7981 gccattttcc cactgacgcc agctttcact gtcaccggtc tgggcaatcc aggtcgcgca
     8041 ttcatcaata gtcatggcaa aaatatggcg aagtgcctgg agttcataag cgttcatcgt
     8101 gtaatggctg catgaaataa acaataaaat ataaataaca ttatcagcct gctgacggct
     8161 tgaagagagt cacagtatct tgtgcaacat tattggaaag agtgaggggc gaggtagcga
     8221 agcggaaaaa tgttaaaaaa aagcccatcg tgggagatgg gcaaagacta cacacagcaa
     8281 ttcgttgttt cactcagggg atttccatgc ttataaatca atatgttgat ttataaccgt
     8341 aagctaatag taggcatacg gactttttgc gtcgatcaga ttcgtctcat tagtttaaaa
     8401 actgtaaaga atttgcgaca ggtactacca gctgaatggc ggtaagcagg aaaatatggg
     8461 gaagagcaag caaatatcaa ttagcggggg ttaacaatca ggcggcagag ccgcctgatg
     8521 agaaattact tattcggttt ctcgatttcg ccaagttctt cgagaatttc ttccggttgg
     8581 tccgtcggcg gcgggacttc ttccacccac gcggcaaaca gacgccagga aacggctaac
     8641 agaaccggac caataaacag accgatcatc ccgaaagcaa tcaaaccacc aataacacca
     8701 gagaggatca ggatcagcgg taaatcggca cccatgcgaa ttaacattgg gcggatgacg
     8761 ttatccagcg tgccaaccac accgctccac actaacaata ccgttcccca ggtggtatcg
     8821 ccagtccagt agagccagat aatcgccgga atcagtaccg gcaacgggcc aagctggaca
     8881 aggcaggaga ggatcattaa caccgttagc aacgttgcat aaggtacgcc ggatacggcg
     8941 aggccgatac cgccaagcac tgcctgtact aacgccgtca ccaccacacc cagcgccacc
     9001 gcgcggatag cctgtgccgc cagcagtacg gcggcatctc cgcgaacgcc tgccagacgg
     9061 gttgcaaaat ggcgaatgcc ttgtgccacc tgttcaccgc gccagtacag cagggcactg
     9121 aagagaagca tcagcgcaca atgcaccata aagcgcccga tatgcgccgc ctgcccaacg
     9181 aaccaggtgg tggtggtgcc aatataaggg cggactttcg ccatgatcgc cgtccccccc
     9241 atatccagca agttgtgcca gcctgcatac agcttcgcgc caatcaccgg aatggtatta
     9301 agccacgcca gatcgggtaa cgtcatgtcg ccgctggaaa tggctttaat tagcgggccg
     9361 ctgccgtcga cgatactgtt aaccagcaaa gcaataggga tgataaacac catcactaat
     9421 aacagcgtca tcaccagaac ggcgagagag cggcggccaa acatgatctt ttgcaaacgt
     9481 aacaataccg gccaggtggc gataaccacc gtaccggccc atgcaaagcc gagaataaag
     9541 ggttgaacaa tccacagaca tgccacaatc atgatggcta aaaacagcac cgaaagcaga
     9601 atttgtgcga catccctggg ctgacgaaca tttaccataa ctacttttca ccttttttgt
     9661 gcgtcaaatc gtcgacgcga cgtgaacacg cgaaactcac ctgcataatg atgagcaatt
     9721 tcagcggttt ttaacaggcc gattctgccc ttaattctga tgggcgtgtt agaaaaaaat
     9781 gtggtacaac aactcaggca acacgcaaac gatttactcg tcgtatttca agttgcatgt
     9841 gctgcggcta cgttcgttca ccccagtcac ttactattgt aagctcctgg agttcactcg
     9901 cttgccgcct tcctgcaact cgaaatccat agagtaaata aacatcacct ttcagtgcgc
     9961 tattgcagcc gcctgaaaga atagtgtatc gacctacatc acagaccgca ggaaagggtc
    10021 aatataatga ttccacagat ttcccaggca cccggcgtcg ttcaactggt gcttaatttt
    10081 ttgcaagagc tggagcaaca aggttttacc ggcgatacgg cgacaagtta tgccgatcgt
    10141 ctgacaatgt cgaccgacaa cagtatttac caacttctcc ccgatgcggt ggtatttccg
    10201 cgttcaaccg cagatgtggc gctgatcgcc cgtcttgccg cgcaggaacg ctattcatcg
    10261 ctgatcttta ccccccgtgg cggcggcacc ggcactaacg gtcaggcgct caaccagggg
    10321 attattgttg atatgtcccg ccatatgaac cgcatcatcg aaattaaccc tgaagagggc
    10381 tgggtgcgcg ttgaggccgg ggtgataaaa gatcaactca atcagtacct gaaaccgttc
    10441 ggctactttt ttgcgccgga actttcgacc agcaaccggg caacgctcgg tgggatgatc
    10501 aataccgatg cttctggtca gggatcgctg gtctatggca aaacgtcgga tcacgtactt
    10561 ggcgtacgtg cggtgttgtt ggggggcgat attctcgata cgcaaccttt acccgtcgaa
    10621 ctggcggaaa cgctgggtaa atccaatacc acaatcgggc gaatttataa cacggtttat
    10681 caacgttgcc gtcagcaacg ccagttaatc atcgacaact tccccaaact taaccgcttt
    10741 cttaccggtt acgatctgcg tcatgtcttt aacgatgaga tgaccgagtt cgacctgacg
    10801 cgcattctga cgggttcaga agggacgctg gcctttatta ccgaagcgcg gctggatatt
    10861 acgcccttgc ctaaagtgcg ccgtctggtg aacgtcaaat atgactcttt tgactccgcg
    10921 ctgcgtaacg cgccgtttat ggttgaggcg cgggcgcttt cggtagagac ggtggactca
    10981 aaagtgctga atctggcgcg ggaagatatt gtctggcatt ccgtcagcga gttgattacc
    11041 gatgtgcctg acaaagagat gctcgggctg aacattgtgg agtttgctgg tgatgatgag
    11101 gcgctgattg acgagcgagt taatgctctc tgtgtgcggc ttgatgagct gattgccagc
    11161 caacaggcag gtgtgattgg ctggcaggtg tgccgcgagc tggcgggtgt tgaacgtatc
    11221 tatgcgatgc gtaaaaaagc cgttggtctg cttggcaatg ccaaaggtgc cgctaagcca
    11281 attccgtttg ctgaggatac ctgcgtaccg ccggaacatc tggccgatta tattgctgaa
    11341 tttcgcgcgc tgctcgacag ccacggctta agctacggta tgttcggtca cgtcgacgca
    11401 ggtgtcttgc acgtccgtcc agcgctggat atgtgcgatc ctcaacaaga gattttgatg
    11461 aagcaaatct ctgatgacgt agtggcgctg actgcgaaat acggtggttt gttgtggggc
    11521 gagcacggca aaggttttcg cgctgaatac agcccggcgt ttttcggtga ggaacttttt
    11581 gcagaactgc gcaaagtgaa agcggcattt gacccgcata accgactcaa cccagggaag
    11641 atttgcccgc cagaaggtct cgatgcgccg atgatgaaag tggacgcggt gaagcgcggt
    11701 acattcgatc ggcagatccc cattgcggta cgccagcagt ggcgcggtgc gatggagtgt
    11761 aacggcaacg gtttatgctt caactttgat gcccgtagtc cgatgtgtcc gtcgatgaag
    11821 atcacccaga accggattca ttcaccgaaa gggcgcgcaa cgctggtgcg tgaatggctg
    11881 cgtttgttgg cggatcgcgg cgttgatcca ctcaaactgg aacaagaact gcctgaatcc
    11941 ggcgtcagtt tgcggacgtt aattgcccgc acgcgcaata gctggcatgc gaataaaggc
    12001 gaatatgact tctcacacga agtcaaagag gcgatgtcgg gctgtctggc ctgtaaagcg
    12061 tgttcgaccc agtgccccat caaaattgat gtgccggagt ttcgctctcg ttttctgcag
    12121 ctctatcaca cccgttattt acgcccgctg cgcgaccacc tcgtcgctac ggtcgagagc
    12181 tacgcgccgc tgatggcacg cgcgccgaaa acctttaact tcttcattaa ccagccgctg
    12241 gtgcgcaaac tctcggaaaa acatatcggc atggttgatt tgccgctgct gtcggtcccc
    12301 tcgctacaac aacaaatggt ggggcatcgc tcggcaaaca tgacgctgga acagcttgaa
    12361 tctctcaatg cagagcagaa agcgcgcaca gtgttggtgg tgcaggaccc ctttaccagc
    12421 tattacgatg cgcaagtggt ggcggatttt gtccgtctgg tcgaaaaatt aggtttccag
    12481 cctgtgttac tgccattttc gccaaatggc aaagcccagc atattaaagg ttttcttaat
    12541 cgttttgcga agacggcgaa aaagacggcg gatttcctca accgtatggc gaagctgggt
    12601 atgccaatgg tgggcgtcga tccggcgctg gtactttgtt atcgcgatga atataaactg
    12661 gcgctgggcg aggaacgtgg cgagtttaac gtcttactgg cgaatgaatg gctggcaagc
    12721 gcacttgagt cacagccagt ggctacagtc agcggtgaat catggtattt ctttggtcac
    12781 tgtaccgaag ttaccgcctt gccgggtgcg ccagcacaat gggccgcgat atttgcccgt
    12841 tttggcgcga aactggaaaa tgtcagcgtg ggttgctgcg gcatggcagg gacttacgga
    12901 catgaagcga aaaaccataa aaattcgctc gggatctatg agttatcctg gcatcaggct
    12961 atgcagcgac tgccgcgtaa ccgctgtctg gcgaccggat attcctgccg tagccaggta
    13021 aaacgggttg aaggcacggg ggtacgccat cctgtgcagg ctttactgga gattattaaa
    13081 tgatatggaa acggaaaatc accctggaag cactgaatgc tatgggtgaa ggaaacatgg
    13141 tggggttcct ggatattcgc tttgaacata ttggtgatga cacccttgaa gcgacaatgc
    13201 cagtagactc gcggacaaag cagcctttcg ggttgctgca tggaggagca tccgtggtac
    13261 tggccgaaag tatcggttcc gttgccggtt atttatgtac cgaaggtgag caaaaagtgg
    13321 ttggtctgga aatcaatgct aaccacgtcc gctcggcacg agaagggcgg gtgcgcgggg
    13381 tatgcaaacc gttgcatctc ggttcgcgtc accaggtctg gcagattgaa atcttcgatg
    13441 agaaagggcg tttgtgctgt tcgtcacgat tgacgaccgc cattttgtga cgttgtcacc
    13501 agaaaagtgt gacgcatatt gcctttggtc attatctgca cagtaaacgg tggtatactg
    13561 tgcaggtctt gtctaaaagc gaggatgcta tgtctactca acttgatccc acccagctgg
    13621 ccattgaatt tttacgccgt gaccagagca atctttcacc tgcccaatat ctcaaacgtc
    13681 tgaaacaact agaacttgaa tttgctgacc tgcttactct ctcttctgct gaattaaaag
    13741 aagaaatcta ctttgcctgg cgtctgggcg tgcattgatc ctgacttaat gccgcaagtt
    13801 cgccaaatcg acctggatta acctccgtcc aggttcgatg accttcttaa tactgcttat
    13861 ctaccgcctg atattgttca aaaataatac taatcgcagt ttgtgttaaa acggcgggtt
    13921 agctttatga gattattctt atcgcccctt caagagctaa gccactgaga gtgccggaga
    13981 taagcgccgg atggggtaga aacccttaag cctgtgttgc acagacttaa gggttttctt
    14041 atttctgaac gctgcacttt tttacctatc tcttaaatag tcattttcaa tacaactttt
    14101 tctttttcat cacagcctta atgctcctta gataataacc attatctaac aatgagatac
    14161 ctaattctta gcgtgcctgt taacccacac atcagggtct atgcttatta aaagcaggca
    14221 aaaagcatgt tttacggtaa agcccctgcg tttgctgggt tgtactgata atcattatca
    14281 ctaacatgct gttatacgct gaaagcgatg aagtgaggta aatcgatgga catgcattca
    14341 ggaaccttta acccacaaga tttcgcctgg caaggcttaa cgctgacacc cgcagcggcg
    14401 atacacatcc gtgagctggt ggcaaagcag ccgggtatgg tcggcgtgcg cttaggcgtg
    14461 aagcaaacgg gctgcgcggg ctttggctat gtgctcgaca gtgttagcga gccggacaaa
    14521 gacgatctgc tgtttgaaca cgacggcgcg aagctgtttg tcccgctgca agcgatgccg
    14581 tttattgatg gcacggaagt cgatttcgtt cgtgaaggac ttaatcagat attcaaattt
    14641 cacaacccta aagcccagaa tgaatgtggc tgtggcgaaa gctttggggt ataggcggta
    14701 ctatgtctcg taatactgaa gcaactgacg atgtcaaaac ctggaccggc ggcccgctga
    14761 attataaaga aggattcttc acccagttag ccaccgatga gctggcaaag gggataaacg
    14821 aagaggtggt gcgcgcaatt tcggcgaagc gtaatgagcc ggagtggatg ctgaagtttc
    14881 gtctaaacgc ctatcgcgca tggctggaga tggaagaacc gcactggttg aaagcgcact
    14941 acgacaagct gaattatcag gattacagct actactcagc accatcgtgc ggtaattgtg
    15001 acgacacttg cgcgtctgaa cctggcgcgg tgcagcaaac tggcgcgaac gcctttttaa
    15061 gtaaagaggt ggaggcggcg tttgagcagt tgggcgttcc cgtgcgggaa ggcaaagagg
    15121 tggcggtgga tgccattttc gactcagttt cggttgccac tacttatcgc gaaaaactgg
    15181 cggagcaggg aattattttc tgttcctttg gtgaggcgat ccacgatcac ccggaactgg
    15241 tgcgtaaata tctcggcacc gtggtgccgg ggaatgacaa cttctttgcc gcgcttaatg
    15301 cggcggtagc ctctgacggt acgtttattt atgtgcctaa aggcgtgcgc tgcccgatgg
    15361 aactttccac ctattttcgc attaacgcgg aaaaaaccgg gcagtttgag cgcaccattc
    15421 tggtggccga cgaagacagc tacgtcagct acattgaagg ctgttccgct ccggtgcgtg
    15481 acagctatca gttacacgcg gcagtggtgg aagtcatcat ccataaaaac gccgaggtga
    15541 aatattccac ggtacaaaac tggttccctg gcgataacaa caccggcggt attctcaact
    15601 tcgtcaccaa gcgtgctttg tgcgaaggcg aaaacagcaa aatgtcatgg acgcaatcag
    15661 aaaccgggtc cgcgattacg tggaaatatc ccagctgcat tttgcgcggc gataactcca
    15721 ttggtgagtt ttactcagtg gcgctgacca gcggtcatca gcaagcggat accggcacca
    15781 agatgatcca catcggtaaa aacaccaaat cgaccattat ctcgaaaggg atctctgccg
    15841 gacatagtca gaacagttat cgcggcttag tgaaaatcat gccgacggca accaatgcgc
    15901 gcaatttcac tcagtgcgac tcaatgctga ttggcgctaa ttgtggggcg cataccttcc
    15961 cgtatgtcga atgtcgcaat aacagcgcac aactggagca cgaggcaacg acatcacgta
    16021 ttggtgaaga tcaactgttt tactgcctgc aacgcgggat cagcgaagaa gacgccatct
    16081 cgatgattgt taacggtttc tgcaaagacg tgttctcaga actgccgctg gaatttgccg
    16141 ttgaagcaca aaaactcctc gccatcagtc ttgaacacag cgtcggataa ggaataaaca
    16201 tgttaagtat taaagattta cacgtcagcg tggaagataa agctatcctg cgtggattaa
    16261 gcctcgccgt tcgtcccggc gaagttcacg ccattatggg gccaaacggt tcgggcaaaa
    16321 gtaccttatc ggcaacgctt gccgggcgcg aagattatga agtgacgggc ggcacggttg
    16381 agttcaaagg caaagatttg cttgcactgt cgccggaaga tcgtgcaggc gaaggcatct
    16441 ttatggcctt ccagtatccg gtggagattc caggcgtcag taaccagttt ttcctgcaaa
    16501 cggcacttaa tgcagtgcgc agctatcgcg gccaggaaac gctcgaccgc tttgattttc
    16561 aggatttgat ggaagagaaa atcgctctcc tgaagatgcc ggaagattta ttaacccgtt
    16621 cggtaaacgt tggtttttcc ggcggcgaga aaaagcgcaa cgatattttg caaatggcgg
    16681 tgctggaacc ggagttatgc attcttgatg agtcggactc cgggctggat attgacgcat
    16741 taaaagtggt cgccgatggc gtgaactcgc tgcgtgatgg caagcgctca ttcatcattg
    16801 ttacgcacta ccaacgcatt ctcgactaca tcaagcctga ttacgtccat gtgctatatc
    16861 ggggacgaat tgtgaaatcc ggcgatttca cgttggtcaa acaactggag gagcagggtt
    16921 atggctggct taccgaacag cagtaacgcg ctgcaacagt ggcatcactt gtttgaagct
    16981 gaagggacaa aacgctcacc gcaagcacag cagcatttac aacaattgct gcgtaccgga
    17041 ctgccgacac gtaaacatga aaactggaaa tatacgccgc tggaagggct gaccaatagc
    17101 cagtttgtca gcattgcggg agagatatcc ccacagcagc gagatgcctt agcgttaacg
    17161 ttagacgctg tgcggctggt atttgtcgat ggacgttacg tgtcagcact gagcgatgcg
    17221 actgaaggca gcgggtatga agtgagcatt aacgacgacc gtcagggggt acccgacgct
    17281 attcaggcgg aagtgtttct gcatttgacg gaaagcctgg cacaaagcgt gacgcatatc
    17341 gccgtgaagc gcggtcaacg gccggcaaag ccattgctgt taatgcatat tacccagggc
    17401 gtggcaggtg aagaggtcaa cactgcccat taccgacatc atctggatct ggcggaaggt
    17461 gccgaagcaa cggtgatcga acattttgtc agccttaatg atgctcgtca cttcaccggc
    17521 gcacggttca ctatcaatgt cgcagcgaac gcccacttgc agcatatcaa gctggcgttt
    17581 gaaaacccgg tcagtcacca ctttgcccat aacgatttgt tgctggctga cgatgccacc
    17641 gcatttagcc atagtttcct gctgggtggc gcagtgttac gacacaacac cagtacgcaa
    17701 ctcaatggcg aaaacagcac gctgcggatc aatagcctgg cgatgccggt gaaaaacgag
    17761 gtgtgtgata cccgtacctg gctggaacac aataaaggtt tttgtaacag ccgacagttg
    17821 cacaaaacta tcgtcagcga caaaggccgc gcggtattta acggtttgat caacgtcgcg
    17881 cagcacgcca tcaaaacgga tggtcagatg accaacaata atctgctgat gggcaaactg
    17941 gcagaagtgg atacgaaacc gcagctggaa atctatgcag atgatgtgaa atgcagccac
    18001 ggcgcgacgg tggggcgtat tgatgatgaa cagatgttct atctgcgctc gcgcgggatc
    18061 aatcagcagg atgcccagca gatgatcatt tacgcctttg ctgccgaact gacggaagca
    18121 ctgcgtgatg aggggcttaa acagcaggtg ctggcccgaa tcggtcaacg gctgccagga
    18181 ggtgcaagat gactttttcc gtcgacaaag tgcgggccga ctttccggtg ctttctcgtg
    18241 aggtaaacgg tttgccgctg gcttatctcg acagcgccgc cagtgcgcag aaaccgagcc
    18301 aggtgattga cgccgaggcc gagttttatc gtcatggcta cgcggcggtg catcgcggta
    18361 ttcatacctt aagcgcccaa gcgaccgaga aaatggagaa cgtgcgcaag cgggcatcgc
    18421 tgtttattaa tgcccgttcg gcggaagagc tggtgttcgt ccgcggcacg acggaaggga
    18481 tcaatctggt cgccaatagc tggggtaaca gcaatgtgcg ggcgggcgat aacatcatca
    18541 tcagtcagat ggagcaccac gctaacattg ttccctggca gatgctttgc gcacgcgttg
    18601 gcgcagagct gcgtgtgatc ccgctcaatc ccgacggtac gttgcaactg gagacgctgc
    18661 ctacgctgtt tgatgagaaa actcgcctgc tggcaattac tcatgtctcc aacgtgcttg
    18721 gcacagaaaa tccactggcg gaaatgatca cgcttgcgca ccagcatggc gcaaaagtgc
    18781 tggtggatgg cgctcaggcg gtgatgcatc atccggtgga tgttcaggcg ctggattgcg
    18841 acttttacgt gttctccggg cataaactgt atggccccac cggaattggc attctttatg
    18901 tgaaagaagc cttgttgcag gagatgccgc cgtgggaagg gggcggttct atgatcgcca
    18961 ccgtcagcct gagtgaaggc acaacctgga ccaaagcacc ttggcggttt gaagccggta
    19021 cacccaatac cgggggcatc attggtcttg gcgcggcgct ggagtatgtt tcggcgctgg
    19081 ggcttaataa catagccgag tatgaacaga atctgatgca ctacgcgcta tcacagctgg
    19141 aatctgtacc ggatctcact ctctatggtc cgcagaacag gcttggcgtt attgctttta
    19201 atcttggtaa acaccacgcc tatgatgttg gcagttttct cgataactac ggcattgctg
    19261 tgcgtaccgg acatcactgc gctatgccat taatggccta ttacaacgtc cctgctatgt
    19321 gtcgggcgtc gctggccatg tataacaccc atgaagaagt ggatcgtctg gtgaccggac
    19381 tgcaacgtat tcaccgtctg ctgggataac agggaggcac tatggcttta ttgccggata
    19441 aagaaaaatt gctgcgtaat tttttacgct gcgccaattg ggaagagaaa tatctctaca
    19501 ttattgagct gggccagcgt ctgccagaat tacgcgacga agacagaagc ccacaaaata
    19561 gcattcaggg ctgccagagt caggtgtgga ttgtcatgcg ccagaatgcg cagggaatta
    19621 ttgaattgca gggcgacagc gatgcggcga ttgtgaaagg gcttattgcg gtcgtcttta
    19681 ttctctacga tcagatgacg ccgcaggata ttgtcaattt cgatgtgcgt ccgtggtttg
    19741 aaaaaatggc gctcacccaa catctcaccc catctcgttc acaaggtctg gaagcgatga
    19801 ttcgcgcaat tcgcgccaaa gccgctgcac ttagctaaac taaagggaca gctttcatcc
    19861 ggttggctca cattgccgga tggtcaatgt ttacactgac ctgcacagga gtgtttcggt
    19921 ttcaggtgaa cataagcgcc atccgacgtt tcagcgtgag tctccggcaa atacaggagg
    19981 tttacaatga aacgcgcgtc tttgcttaca ctcacgctta tcggcgcttt tagcgccatc
    20041 caggctgcct gggcggttga ttatccgcta ccaccaaccg gaagccgact ggttgggcaa
    20101 aatcaaacgt atacggtgca ggaaggggat aaaaaccttc aggccatcgc ccgacgtttt
    20161 gatactgcgg caatgttgat ccttgaagcc aataacacta tcgccccggt gccaaaaccc
    20221 ggtacgacga taactattcc ttcgcaactg ttattacctg atgcgccgcg tcaggggatt
    20281 atcgttaacc ttgcagagct gcgcctttat tattatccgc cgggagaaaa tatcgtgcag
    20341 gtttatccga taggtattgg cttgcagggg ctggaaacgc cggtgatgga aacgcgtgtg
    20401 gggcagaaaa tccctaaccc aacctggacg cctactgcag gcattcgtca acgttcgctg
    20461 gagcgtggta ttaaattacc gccagtcgtt cctgccggac caaataaccc gctaggacgt
    20521 tacgcactgc gcctcgcgca tggtaatggc gaatacctca ttcatggcac cagtgcgccg
    20581 gacagcgtcg gtttgcgcgt cagttcaggg tgtattcgca tgaatgcacc ggatattaaa
    20641 gccttgttct ccagcgtgcg gacgggaacg ccggtgaaag tgatcaacga acctgtgaaa
    20701 tattccgtgg agcctaacgg gatgcgttat gttgaagtac atcgaccact atcggcagaa
    20761 gaacagcaga acgttcagac aatgccatac acactgccag caggctttac gcaatttaaa
    20821 gacaataagg ctgtagatca gaagttagtc gataaagcgt tgtatcgtcg ggcagggtat
    20881 ccggtttcgg tgagcagtgg agcaactccc gcagccagca atgcgccttc agtagagtca
    20941 gcgcagaatg gtgaaccaga gcaagggaat atgttacgcg cgacgcagta gcggtaaacg
    21001 gcagacaaaa aaatggcgca caatgtgcgc catttttcac ttcacaggta ctattacttg
    21061 cggtatttag tagccatgtt gtccagacgc tggttagcac gagctgcgtc atctttagca
    21121 gcctgaacgt cggaacgcat tgcgttcacg tcgttgctca gctggtcaac tttagcgttc
    21181 agagtctgaa cgtcagaaga cagctgatcg attttagcgt tgctggagca acctgccagc
    21241 agagtagaac ccaggattac cgcgcccagt accagtttag tagctttcat tattaatacc
    21301 ctctagattg agttaatctc catgtagcgt tacaagtatt acacaaagtt ttttatgttg
    21361 agaatatttt tttgatggga aggcacttat ttttgatcgt tcgctcaaag aagcatcgaa
    21421 cgctgggttt tagataaaaa aattgagaga aaacaggatg cttccatcgg attcatctta
    21481 gataaagtga gattatatag tgaaatccga tttgattttt tattgcttct ggttatcgat
    21541 t
//
LOCUS       DAZGLJ010000061        21324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000061 DAZGLJ010000000
VERSION     DAZGLJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.061"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            20..6127
                     /gene="irp2"
                     /locus_tag="ACHK49_002062"
     CDS             20..6127
                     /gene="irp2"
                     /locus_tag="ACHK49_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HFO9262710.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDVARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLQPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     ALAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAYWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLVGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     IVRDGQQQVLEQTPPWVIPAHTLHTPEEALRVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPDFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTQRPRPVRRRQRRI"
     gene            6215..15697
                     /gene="irp1"
                     /locus_tag="ACHK49_002063"
                     /pseudo
     CDS             6215..15697
                     /gene="irp1"
                     /locus_tag="ACHK49_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
     gene            15694..16794
                     /gene="ybtU"
                     /locus_tag="ACHK49_002064"
     CDS             15694..16794
                     /gene="ybtU"
                     /locus_tag="ACHK49_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HFO9262711.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDMVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLYVADHQQNPHSLWRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSNLAGEHPPEACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            16791..17594
                     /gene="ybtT"
                     /locus_tag="ACHK49_002065"
     CDS             16791..17594
                     /gene="ybtT"
                     /locus_tag="ACHK49_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HFO9262712.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            17598..19163
                     /gene="ybtE"
                     /locus_tag="ACHK49_002066"
     CDS             17598..19163
                     /gene="ybtE"
                     /locus_tag="ACHK49_002066"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HFO9262713.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETMSDDFTPLFSLHGER
                     QPDVSTTALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLPVAHN
                     FPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQAREWE
                     DSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHATILH
                     SQGRPLSPLDEICIVDQDENDVAPGETGQLLTRGPYTISGYYHAPAHNAQAFTAQGFY
                     RTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAAPDTL
                     LGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKIDKKRL
                     TALAVDRYRHSAQ"
     gene            19294..21315
                     /gene="fyuA"
                     /locus_tag="ACHK49_002067"
     CDS             19294..21315
                     /gene="fyuA"
                     /locus_tag="ACHK49_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HFO9262714.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIEDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPDGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
BASE COUNT         4278 a         6541 c         6170 g         4335 t
ORIGIN      
        1 cgtgccatca ggaggaagaa tgatttctgg cgcaccatct caggattcgc tgttaccgga
       61 caaccgccac gcggctgatt accaacaatt acgcgagcgg ctcatacagg aactgaattt
      121 aacgccgcag cagttacatg aagagagcaa cctgatccag gccggtctgg attccataag
      181 attgatgaga tggttacact ggtttcgtaa aaatggctac cgccttaccc ttcgcgagct
      241 gtatgccgcc cccacgctgg cggcatggaa ccagttaatg ctcagccggt cgccggagaa
      301 cgcggaagaa gaaacgccgc ccgacgaatc atcctggccg aacatgaccg aaagtacccc
      361 cttcccattg acgccagtac agcacgccta cctgacgggc cgcatgccgg ggcagacgct
      421 tggcggcgtg ggttgccacc tgtatcagga gtttgaaggc cattgtctga cggcgtcgca
      481 gctggagcag gccatcacga ccttgctgca acgccaccca atgctgcata tcgcctttcg
      541 ccccgacggg cagcaggtct ggctaccgca accttactgg aacggcgtca ccgttcatga
      601 tttacgccat aacgacgctg aaagccgcca ggcctatctg gacgcactgc gccagcgcct
      661 gagccaccgt cttttacgcg tggaaatcgg cgaaacgttt gattttcagc tgacgctctt
      721 gccggacaat cgccaccgcc tccatgtcaa tattgacctg ctgattatgg atgcctccag
      781 ctttacgctt ttcttcgatg agcttaacgc cctgctggcc ggagaatcgc tgccggctat
      841 cgacacccgc tatgatttcc gctcgtattt gctgcaccag cagaagatca atcaaccact
      901 gagagacgtc gcgcgcgctt actggctggc gaaagcatcg acgcttcccc ccgcgcccgt
      961 cttgccgctg gcctgcgaac ccgccacgct acgtgaagtc cgtaataccc gacgccgcat
     1021 gattgtcccg gcaacacgct ggcacgcctt tagcaaccgg gccggcgagt atggcgtgac
     1081 gccgacaatg gcactggcga cctgtttttc tgccgtgctg gctcgctggg gcggcctgac
     1141 gcgtctgctg cttaacatca ccttattcga ccgccagccg ctgcaaccgg cggttggcgc
     1201 gatgcttgcc gacttcacca atattcttct gctggatacc gcctgcgatg gcgataccgt
     1261 cagcaacctg gcgcgtaaaa accagctcac gtttacggag gactgggagc atcgccactg
     1321 gtccggcgtc gaattactcc gtgaactcaa acgccagcag cgctaccccc acggcgcccc
     1381 ggtggtattt accagcaatc tggggcgttc cctctacagc agccgcgcag aatcgccgtt
     1441 gggcgagccg gaatggggca tctcgcaaac gccgcaggtc tggatagatc atctggcgtt
     1501 cgagcatcac ggcgaggtct ggctacaatg ggacagcaac gacgcgctgt tccctccggc
     1561 gttagtcgaa acattgttcg acgcctactg ccagttgatt aaccaactct gcgatgacga
     1621 aagcgcctgg caaaagccgt tcgcagatat gatgcccgcc agccagcgcg cgatacgcga
     1681 acgggtcaac gccaccggcg cccccattcc cgaaggcttg ctgcatgaag gcattttccg
     1741 tatcgctctg caacagccgc aggcgctggc ggtaacggac atgcgttatc agtggaatta
     1801 tcatgagctg acagactatg cccgccgttg cgcgggcagg ttaatcgagt gcggggttca
     1861 gcccggcgat aatgtggcta tcacgatgtc gaaaggcgca ggacaacttg ttgcggttct
     1921 ggccgtcctg ctggccgggg cggtttacgt tccggtttcg ctggatcagc ctgccgcacg
     1981 gcgcgagaaa atctacgctg acgccagcgt ccggctggtg ctcatttgtc agcacgacgc
     2041 cagcgccggg tcagacgata ttcccgccct tgcctggcag caggccattg aggcggagcc
     2101 gatcgccaac ccggtagtac gcgcccccac gcaaccggcc tacattatct acacctccgg
     2161 ctctaccggt acgccgaaag gggtagtcat ttctcaccgg ggagcgctta acacctgttg
     2221 cgatatcaat acccgctatc aggttggccc gcatgacagg gtgctggccc tctccgccct
     2281 acattttgat ttatcggttt acgacatttt tggcgtactg cgcgcgggcg gcgcgctggt
     2341 gatggtgatg gaaaatcaac ggcgcgatcc tcacgcatgg tgtgagctga tccagcgcca
     2401 tcaggtcacg ctctggaaca gcgtcccggc gctgttcgat atgctgctga cctggtgtga
     2461 aggtttcgcc gacgccacgc cggaaaacct gcgcgcagtg atgctttccg gcgactggat
     2521 cgggcttgac ctccccgccc gttatcgggc cttccggcca caaggacaat ttatcgcgat
     2581 gggcggcgcc accgaggcgt ctatctggtc taacgcctgc gaaattcacg acgtccccgc
     2641 ctactggcgc tccatccctt acggttttcc gctaaccaac caacgctacc gggtggtgga
     2701 tgaacagggc cgggactgcc ctgactgggt gccgggtgaa ttatggattg gcggcattgg
     2761 ggtcgcggaa ggctatttca acgatcccct gcgtagcgag cagcaatttt tgacgctccc
     2821 ggacgagcgc tggtatcgca ccggcgatct cggctgctac tggccagatg gcacaatcga
     2881 gttcctcggt cgtcgcgaca agcaggtcaa agtcggagga tatcgcatcg agctgggcga
     2941 aatcgaaagc gcgctcagcc agctggtggg ggtgaaacaa gcaaccgttc tggcgatcgg
     3001 cgaaaaagaa aaaacgctgg cggcatacgt tgttcctcag ggcgaggctt tttgcgttac
     3061 cgatcatcgg aacccggcac tgccgcaggc gtggcacacg cttgcgggaa cgttgccctg
     3121 ttgcgccatc tcgccagaga tctccgcaga acaggtagcc gatttccttc agcatcgcct
     3181 gctaaaactg aagccgggtc acaccgctgg cgccgatcct ctccccctga tgaactcact
     3241 cgctatccag ccgcgctggc aggccgtggt ggaacgctgg ttagcatttc tggtgacaca
     3301 acggcgactg aagcccgctg ctgaaggtta tcaggtctgc gctggtgaag aacgcgagga
     3361 tgagcacccg cacttcagcg gacatgattt aacgttatcg caaattcttc gcggtgcccg
     3421 taacgaactg tcgttactga acgacgcgca gtggtcgccg gaaagcctgg cctttaacca
     3481 tccggccagc gccccgtata ttcaggaact ggcgacaatt tgccaacagc ttgcacagcg
     3541 cttacagcgc ccggtacgcc tgcttgaggt gggaacccgc actggccgcg ccgcagaatc
     3601 gctgttagca cagctcaacg ccggacagat tgagtatgtc gggcttgagc agagccagga
     3661 gatgctgctg agcgcccggc agaggctcgc cccctggcct ggcgcccgtc tgtccctctg
     3721 gaatgcagac acgctggcgg cgcacgctca ctcggcggac attatctggc ttaataacgc
     3781 cctgcatcgt ctgctgccgg aagatcccgg gctccttgcg acattacaac agcttgccgt
     3841 tcccggcgcg ctgctctacg tgatggagtt tcgccagtta acgccgtccg ccctactcag
     3901 cacgctcctg ttaaccaatg ggcagccgga ggccttgctg cataacagcg ccgactgggc
     3961 ggcattattt agcgcggccg gcttcaactg tcagcatggc gatgaggtcg cggggttaca
     4021 acgcttcctc gtacaatgtc ctgacaggca ggtgcgccgc gatccccgtc aacttcaggc
     4081 cgccctcgcc gggcgtctgc cggggtggat ggtgccgcaa cggatcgtct tcctcgacgc
     4141 cttaccgctg acagctaacg ggaaaattga ctaccaggcg ctgaagcgtc gtcatacccc
     4201 tgaagcggaa aacccggccg aagcggattt accccagggc gacattgaaa aacaggttgc
     4261 cgccctctgg cagcaactct tatcaactgg caatgtcacc agagaaaccg acttcttcca
     4321 gcaaggcggc gatagcctgc tggcgacccg tctgaccggg caacttcatc aggcaggtta
     4381 tgaagcgcaa ttaagcgacc tgtttaatca tccccggctg gcggattttg ccgccacgct
     4441 gcggaaaacc gacgtcccgg tcgaacaacc attcgtccac tcccctgaag atcgctacca
     4501 gccctttgcg cttaccgacg tgcagcaggc ttacctggtg gggcgtcagc cgggctttgc
     4561 cctgggcggc gtcggctcac atttctttgt tgaatttgaa attgccgatc tggacctcac
     4621 ccggctggag acggtctgga accgattaat cgcccgccac gatatgctgc gcgccatcgt
     4681 gcgtgatgga cagcaacagg tgctcgaaca gacgcccccc tgggtgatac ccgcacacac
     4741 cctccatacg cctgaagagg cgttgcgggt gcgcgaaaaa ctggcgcatc aggtactcaa
     4801 ccccgaagta tggccggtat tcgatctcca ggtcggatac gtggacggga tgcctgcccg
     4861 cctgtggctg tgtctggata acctgttgct tgacggtctg agcatgcaga tcctgctggc
     4921 ggagctggag cacggctacc gctacccgca acagctgctt ccgccgctgc ccgtcacctt
     4981 cagggattat ctgcaacaac cctcgctaca gtcgcccaat ccagattctc tggcatggtg
     5041 gcaggcgcag cttgatgata ttcctccggc gcctgcgttg ccgctgcgct gcttgcctca
     5101 ggaggttgaa acaccgcgct tcgcccgcct gaacggcgca ctggacagca cgcgctggca
     5161 tcggctgaaa aaacgggcgg ctgacgccca tctcaccccg tcggccgtac tgttgtcggt
     5221 gtggtcaacg gttctctctg catggagtgc acagcctgac ttcacgctta accttacgct
     5281 tttcgacagg cgaccgctgc acccgcaaat caaccagatt ctgggcgatt tcacctcgct
     5341 gatgctgctg agctggcatc ccggcgaaag ctggctgcac agcgcgcagt cactacagca
     5401 gcggctgagc cagaacctca accaccgcga tgtgtcagcc atccgcgtga tgcgtcaact
     5461 ggcgcaacgg caaaacgtgc cagccgttcc gatgcccgtc gtctttacca gcgcgctggg
     5521 ctttgagcag gataacttcc tcgcccggcg taatctgctc aaaccggtct ggggcatctc
     5581 ccagacgccg caggtctggc tcgatcacca gatttatgaa tccgaaggcg aactgcgctt
     5641 taactgggat tttgtcgccg cgctgtttcc tgccgggcag gtggagcgcc agtttgaaca
     5701 gtattgcgca ttgctaaacc gaatggccga ggatgaaagc ggctggcaac tgccgctcgc
     5761 cgcgctggtg cctcccgtta aacacgcagg gcaatgcgca gagcgctcac cgcgcgtatg
     5821 ccctgagcac tctcagccac acattgcggc ggacgagagc accgtcagcc tgatttgcga
     5881 cgccttccgc gaggtggttg gcgagtctgt cacgcccgca gaaaacttct ttgaggcggg
     5941 cgcaacgtcg ctgaatctgg tgcaactgca cgttttgtta caacgtcacg aattttccac
     6001 cctgacgttg cttgacctct tcacccaccc ttctcctgct gccctggccg attatctggc
     6061 cggcgtcgcc acggtggaga aaacacaacg acctcgccct gttcgccgtc gtcagcggcg
     6121 gatatagcgc gaagcaaact gattttcccc ggaacgccat cgcgaacgca tggcgttcca
     6181 ttgactttgt gaatcttagg aaacgggacc gattatggat aacttgcgct tctcttctgc
     6241 gccgacagca gattccattg atgcatcgat cgctcaacac tacccggact gcgaacctgt
     6301 cgcggttatc ggctacgcct gccattttcc tgaatcgccg gatggcgaaa cgttctggca
     6361 aaatctgctg gaaggtcgtg aatgcagccg acgctttacg cgcgaagagc ttctggccgt
     6421 cggtctggat gccgccatca ttgacgatcc tcattatgtc aatatcggtt cggtgttaga
     6481 caacgccgac tgcttcgacg ccaccctgtt tggctattcg cgacaggaag cggagtcgat
     6541 ggacccgcag cagcgcctgt ttttgcaggc ggtctggcat gcgctggaac atgccggtta
     6601 tgcccccggc gccgtccccc ataagaccgg cgttttcgcc tcttcccgga tgagtaccta
     6661 ccccggtcgc gaagcattga acgtgacaga agtcgcgcag gtaaaaggtc tgcaatctct
     6721 gatgggcaat gataaagact atattgccac ccgcgccgcg tacaaactca acctgcacgg
     6781 cccggcgtta tcggtacaga ccgcctgctc cagctcgctg gttgccgtgc atctggcctg
     6841 tgaaagcctg cgcgctggcg aatccgatat ggcggttgcc ggcggcgtgg cgctctcttt
     6901 cccccagcag gcaggctacc gctaccagcc cggaatgatt ttctctcctg atggtcactg
     6961 tcgtcccttt gacgcctcgg ctgagggcac ctgggccggt aacggtctcg gctgcgtggt
     7021 gctgcgtcgc ctgagagacg cgctgctgtc aggcgatccg attatctcgg tgatcctctc
     7081 cagcgcggtc aacaacgacg gcaacagaaa ggtcggctat accgcccctt ccgtcgcagg
     7141 gcaacaggca gtcatcgaag aggcgttaat gctggcggcc atcgacgaca ggcaggtagg
     7201 ttacattgaa acccacggca ccggcacacc gctgggcgac gcgattgaaa ttgaagcgtt
     7261 acgcaacgtc tatgcgcctc gcccgcagga tcagcgctgt gcgctcggtt ccgtgaaaag
     7321 taacatgggc catctggata ccgcggcggg cattgccgga ctgctgaaaa ccgttctggc
     7381 agtcagtcgc gggcaaattc ctcccttact gaattttcac acccccaacc cggcgctgaa
     7441 acttgaagag agccccttta ccataccggt gtcggcacag gcatggcagg acgaaatgcg
     7501 ctatgcgggc gtctcctcct ttggtattgg cggcaccaac tgccatatga tcgtcgcctc
     7561 gctgcccgac gcgctcaacg cgcgcctccc caatacggat agcggcagaa aaagtaccgc
     7621 gctgctgctc agcgccgcca gcgacagcgc gttgcggcgg ctggcgacgg attatgccgg
     7681 ggcgctgaga gagaatgcgg atgccagctc tctggccttc acagccctgc acgcgcgccg
     7741 tctcgatctc cccttccgcc tggcggcgcc attaaaccgt gaaaccgccg aggcgctcag
     7801 cgcctgggcc ggtgagaaat cgggggcgct ggtttacagc ggccacggcg ccagcggcaa
     7861 gcaggtgtgg ctgtttaccg gccagggctc gcactggcac actatgggcc aaacgatgta
     7921 ccagcactca acggcgtttg ccgacacgct ggatcgctgt ttttccgcct gtagcgaaat
     7981 gctcacgcca tcactgcgcg aagcgatgtt taaccccgat tcggcgcagc tggacaatat
     8041 ggcctgggcg cagccggcga ttgtcgcgtt tgaaatcgcg atggcggcgc actggcgtgc
     8101 tgaaggactg aagccagact tcgccattgg gcattccgtc ggtgaatttg ccgctgccgt
     8161 tgtctgcgga cactatacga ttgaacaggt catgccactg gtttgtcggc gcggcgcgct
     8221 aatgcagcag tgcgcaagcg gcgcgatggt ggcggtattt gcagacgaag acacgctgat
     8281 gccgctggct cgccagtttg agctggatct cgccgccaac aacggtacgc aacatacggt
     8341 attttccggg ccggaagccc gtctcgcggt attttgcgcc acgctctcgc agcatgacat
     8401 taactatcgt cgcctgagcg taaccggtgc ggcgcactcc gctttactgg agccgatact
     8461 cgatcggttc caggacgcct gcgcgggact gcacgcggag ccggggcaaa taccgattat
     8521 ttccacgctc accgccgacg tcattgatga gtcaacgctc aaccaggcgg attactggcg
     8581 ccgacacatg cgccagccgg tgcgttttat ccagagtatt caggtggcgc atcagctcgg
     8641 cgcccgcgtt tttctggaga tggggcccga tgcccagttg gttgcttgcg ggcagcgcga
     8701 ataccgcgat aacgcatact ggatagccag cgcccggcgt aacaaagagg cgagcgatgt
     8761 cctcaatcag gccctgctcc agctttacgc tgccggcgtc gccctaccgt gggccgacct
     8821 gctggcgggc gatggacaac gtatcgctgc gccatgttat ccgtttgata ctgaacgtta
     8881 ctggaaagag cgcgtctccc cggcctgcga gcctgccgac gcagcgctgt ctgccgggct
     8941 ggaggtggcg agtcgcgccg cgacagcgct cgatctccct cgcctggaag cgcttaaaca
     9001 gtgcgccacg cgactgcacg ccatctacgt cgatcaactg gtacaacgct gtaccggcga
     9061 tgccattgag aacggcgtgg acgccatgac catcatgcgc cgtggacgtc tgctgccccg
     9121 ctaccagcag ctactccagc gcctgctgaa taactgcgtg gtcgacggcg attaccgctg
     9181 caccgacggg cgatacgtcc gcgcccgccc cattgaacat caacagcggg aatcactgct
     9241 gacggaactt gccagttatt gtgaaggttt tcaggctatt cccgacacca tcgcccgtgc
     9301 cggcgatcgg ttatatgaaa tgatgagcgg cgcggaagaa ccggtggcga ttatcttccc
     9361 gcaaagcgcc tccgacggcg tggaagtgct gtatcaggaa ttcagctttg gccgctattt
     9421 caaccaaatc gccgccgggg tattacgcgg cattgtccag acgcgtcagc cccgccagtc
     9481 gttgcgtatt cttgaagttg gcggcggaac cggcggcacc accgcgtggc tgctgccgga
     9541 actcaacggc gttccggcac tggagtacca tttcaccgat atctcggcgc tgttcacccg
     9601 tcgcgcccag cagaaattcg ccgactatga ttttgtgaag tatagcgagc tggatctcga
     9661 aaaagaggcg cagtctcagg gtttccaggc acagtcttac gatcttatcg tggcagcgaa
     9721 cgtgattcac gccacccgcc atattggccg cacgctcgat aatctgcgcc ccctgctcaa
     9781 gccgggcggg cgcctgctga tgcgcaaaat cacccagcca atgcgtctgt ttgacttcgt
     9841 tttcggcccg ctggttcttc cgctacagga tctcgacgcc cgcgaaggtg agttattcct
     9901 caccaccgct cagtggcaac aacagtgccg ccacgccgga ttcagcaaag tggcgtggct
     9961 accgcaggat ggcagcccga ccgccgggat gagcgaacat atcattctcg ccacgctgcc
    10021 cggtcaggcg gttagcgccg taacattcac cgcgccatca gaacccgtgt tggggcaggc
    10081 gctgacggat aacggtgatt atctcgccga ctggtctgat tgcgcaggtc agcccgaaca
    10141 gtttaacgcc cgctggcagg aggcctggcg tctgctttca cagcgtcatg gcgacgctct
    10201 gcctgtggaa ccgccccccg tcgccgcccc ggagtggctg gggaaggttc gcttaagctg
    10261 gcaaaacgaa gccttttccc gcggtcagat gcgcgttgaa gcccgtcatc ctgctggcga
    10321 gtggctgccg ctatcgcccg ccgcgcctct tcctgcgccg caaacgcatt atcaatggcg
    10381 ctggacgccc ctcaacgtcg ccagcattga ccatccgctt acctttagct tcagcgccgg
    10441 tacgcttgcg cgcagcgacg agctggcgca atacggcatc attcacgatc cgcacgcctc
    10501 ttcacgactg atgattgttg aggagagcga ggatacgctg gccttagcgg agaaagtgat
    10561 agcagcgctc accgccagcg cagccggatt gattgtggtt actcgccgcg cgtggcgagt
    10621 cgaggaaaat gaagcactct ctgcatccca tcacgcgcta tgggccttgc ttcgcgtcgc
    10681 ggccaacgaa cagccggaac ggttgcttgc cgccatcgat ctcgccgaaa acaccccgtg
    10741 ggaaacgctg catcaagggt tgagcgcagt ctcactatca cagcgctggc tcgccgcacg
    10801 gggtgacacc ctttggctcc cttcactggc gcccaatacg ggatgcgcag ctgaattacc
    10861 ggcaaacgtg tttaccggcg atagccgctg gcatctggtg accggagcgt ttggcggatt
    10921 aggccgcctt gccgtgaact ggctcagaga aaaaggggcg cgacgcatcg ccctgctggc
    10981 atcgcacgtg gatgagtcat ggctacgcga cgtggagggc gggcagacgc gcgtctgccg
    11041 ttgtgatgtg ggcgatgccg ggcaactggc cacggttctt gacgatctgg cggccaacgg
    11101 cggcattgcc ggagcgattc atgccgctgg cgtattggct gacgcgccct tgcaggagct
    11161 tgatgaccac cagctggctg ccgttttcgc ggtaaaagcg caggcggcaa gccagctgtt
    11221 gcaaaccctg cgcaaccacg acggacgcta tcttattctc tactcttccg ctgccgccac
    11281 cctcggcgcg ccgggtcaga gcgcccatgc gctgtcctgc ggctacctgg acgggctggc
    11341 ccagcagttt tccacccttg atgcgccgaa aacgctctct gtcgcctggg gcgcatgggg
    11401 agaaagcggt cgggcggcca cgccggaaat gctggcgacg ctcgccagcc gaggtatggg
    11461 cgcgttaagc gatgccgaag gctgctggca cctggaacag gcggtgatgc gcggcgcccc
    11521 gtggcgactg gcgatgcgcg tttttaccga caaaatgccc ccgttacaac aggctctgtt
    11581 taacatcagc gccacagaaa aagccgcaac gccggtcatt cctcctgctg atgacaacgc
    11641 ctttaacggc agcctgagcg atgaaacagc ggtgatggca tggctgaaaa agcggattgc
    11701 ggttcagcta aggctgagcg atccggcgtc actgcatcca aaccaggatc tgttgcaact
    11761 cggcatggac tcgctgctct tccttgaact cagtagcgat attcagcact acctgggcgt
    11821 acgcatcaat gcggaacggg cgtggcagga tctgtctcct catggactca cgcagcttat
    11881 ctgttctaag ccagaggcga cgcctgccgt ttcgcagccg gaagtgttgc ggcacgacgc
    11941 cgacgagcgt tatgcgccct tccctttgac gcccattcag cacgcctact ggctggggcg
    12001 aacccacctc attggctatg gcggcgtcgc ctgtcacgtc ctgtttgagt gggataaacg
    12061 ccacgatgag ttcgatctag ccatactgga gaaagcatgg aaccagctca tcgcacgcca
    12121 cgatatgttg cgtatggtgg ttgatgccga cgggcagcag cgaatcctgg cgacaacgcc
    12181 ggagtatcac atcccgcgtg acgatctgcg cgcgctttcc ccggaagaac agcgcatcgc
    12241 gctggaaaaa cggcggcatg aactgagcta tcgcgttttg cctgccgacc agtggcctct
    12301 ttttgagctg gtggtcagcg aaatcgacga ttgccattac cgtctgcata tgaacctcga
    12361 ccttttgcag tttgatgtgc agagttttaa agtcatgatg gacgacctgg cgcaggtctg
    12421 gcgcggtgaa acgctggcgc cgctcgctat taccttccgt gattatgtga tggctgaaca
    12481 ggcgcgccga aagacatcgg catggcacga tgcctgggat tactggcagg aaaaactgcc
    12541 gcaactgccc ttagcgccag agctgccgat ggttgagacg cccccggaaa cgccacactt
    12601 caccaccttc aaatcgacga tcggcaagac agaatggcag gccgtgaaac agcgctggca
    12661 gcagcaaggc gtcacaccgt ctgccgcgct gctcacgctg tttgccgcca cccttgagcg
    12721 ctggagccgt accacaacat ttacgctgaa cctgacgttc ttcaatcgcc agccgatcca
    12781 tccgcaaatc aaccagttga ttggtgattt tacctccgtc acgctggttg attttaactt
    12841 ctcagcgcca gtgacgttgc aagagcagat gcaacagacc caacagcgcc tctggcaaaa
    12901 tatggcgcac agtgaaatga acggtgttga ggtgatccgt gagctgggcc gcctgcgcgg
    12961 atcacaacgt caaccgctga tgccggtagt gtttaccagt atgctgggga tgacgctgga
    13021 aggcatgact atcgatcagg cgatgagcca tctgttcggc gaaccctgct atgtattcac
    13081 gcaaacgccg caggtctggc tggatcatca ggtcatggag agcgacggcg agttgatgtt
    13141 tagctggtac tgcatggaca acgtgctgga acccggcgct gccgaggcga tgtttaatga
    13201 ctattgagcc atcctgcaag ccgtcatcgc cgcccctgaa agcctgaaga ctctcgccag
    13261 cggcatcgcc gggcacattc cccgccgacg ctggccgctg aacgcgcagg cggactacga
    13321 cctgcgggat attgagcagg cgacgctcga ataccccggc atccggcagg ccagagcgga
    13381 aataaccgaa cagggcgcgt tgacgctgga tatcgtgatg gccgacgatc cttcgccatc
    13441 agcggcgatg cctgatgagc acgaacttac ccaactggcg ctgccgttgc ctgagcaggc
    13501 gcagcttgat gagctggagg cgacctggcg ctggctggag gcgcgtgcgc tacaggggat
    13561 cgcggctacg ctaaatcgtc acggcctgtt taccacgccg gagatcgccc atcgctttag
    13621 cgcaatagta caggcgctgt ccgcgcaagc gtctcaccag cgtctgctgc gccagtggct
    13681 acagtgtctg acggaaagag agtggttaat ccgcgaaggt gaaagctggc gctgccgcat
    13741 tccgctcagc gagattcctg agcctcagga agcgtgcccg caaagccaat ggagccaggc
    13801 gctggcgcag tatctggaaa cctgcatcgc ccggcacgac gccctcttct ccgggcagtg
    13861 ttctccgctg gaattgctgt tcaacgagca gcatcgcgtt accgacgcgc tgtatcgcga
    13921 caaccccgcc agcgcctgtc tgaatcgcta taccgcgcag attgccgcct tgtgcagcgc
    13981 agaacggatt ctggaggttg gcgccggaac cgcagccact accgcgccgg tgctgaaggc
    14041 cacgcggcag tcgtaccact tcacggacgt ctccgcgcag ttcctcaatg acgccagagc
    14101 ccgtttccat gatgaatcgc aggtgtctta tgccttgttc gacatcaacc agccgctgga
    14161 tttcaccgcc cacccggagg cgggttacga cctgatcgtt gccgtcaatg tgctccacga
    14221 cgccagccat gtcgtccaga cgttgcgcag attaaaactg ttgctgaaag ccggcggacg
    14281 tttgctgatc gttgaagcga cggagcgaaa cagcgtattc cagctggcga gcgtgggctt
    14341 tattgaggga ttaagcggat accgcgattt ccgccgccgg gatgagaaac cgatgctcac
    14401 ccgctccgca tggcaggagg ttctcgttca ggccgggttt gcaaacgagc tggcgtggcc
    14461 cgcgcaggaa tcgtcgccgc tgcgccagca tctgctggta gcgcgttcgc ctggcgtaaa
    14521 tcgcccggat aaaaaagccg tgagccgcta tttacagcag cgctttggca ccggtctgcc
    14581 cattttacag atccggcaaa gagaagcgtt atttacgccg ctgcatgccc cgtctgatgc
    14641 gccgactgag ccagccaaac ccacgccagt tgccgggggg aattcggcgc tggaaaaaca
    14701 ggtggctgaa ctctggcaat cgctgctgtc tcgccccgtg gcaaggcatc acgacttttt
    14761 cgaactgggc ggcgacagcc tgatggcgac aaggatggtc gcgcagctaa accggagagg
    14821 gattgccagg gctaaccttc aggatctgtt caaccattcg acgctgagcg acttctgcgc
    14881 ccatctacag gcggctacgt caggagagga caacccgata cccctttgcc agggcgacgg
    14941 tgaggaaacc ctgtttgtct tccacgcttc agacggcgat atcagcgcct ggctgccgct
    15001 cgctagcgcg ttgaacaggc gcgttttcgg cctgcaagca aaatcgccgc agcgctttgc
    15061 tacgctcgac cagatgatcg atgagtatgt cgggtgcatc cgtcgtcagc agcctcacgg
    15121 cccttatgtg ctggcgggtt ggtcgtatgg cgcgtttctt gcggcgggcg ccgcacagcg
    15181 cctgtacgcc aaaggcaagc aggttcggat ggtgttaatc gatcccgtgt gccgacagga
    15241 tttctgttgc gaaaaccggg cggccctgct gcgcctgtta gccgaaggag aaacgcctct
    15301 ggcactgccc gaacatttcg accagcagac gcccgacagc cagcttgccg actttatcag
    15361 cctcgctaaa acggccggta tggtgtcgca aaacctgacg ctgcaagcgg cagaaacgtg
    15421 gctcgacaac atcgcgcatc tgctgcgttt actgactgag catacgccgg gcgaaagcgt
    15481 tccggtcccc tgtctcatgg tgtatgccgc cgggagaccc gcgcgctgga cgccagcaga
    15541 aaccgagttg cagggctgga taaacaacgc cgacgacgct gtgattgaag ccagccactg
    15601 gcaaatcatg atggaagccc cccacgttca ggcttgtgcg caacacatta cgcgctggct
    15661 ttgcgcaacc tcaacgcaac cggagaacac gttatgatgc cgtccgcctc cccaaaacaa
    15721 cgcgtactga ttgtgggcgc caaatttggc gaaatgtacc tgaatgcctt tatgcagccc
    15781 ccggaggggc tggaactggt cggcctgctg gcgcagggaa gcgcccgttc aagagagctg
    15841 gctcatgcgt ttggcattcc gctgtatacc tcgccggaac agataaccag gatgccggat
    15901 atcgcctgta ttgtcgtgcg ttcgaccgtc gccggaggaa ccggtacgca gcttgccaga
    15961 cactttctga cgcgcggcgt acacgtcatt caggagcatc ccctccaccc ggatgacatc
    16021 agttccttac aaacgctggc gcaggaacag ggctgctgct attgggtaaa cacgttttat
    16081 ccgcatacgc gcgcgggacg cacatggctt cgcgatgcgc agcagctgcg tcgctgcctg
    16141 gctaagacgc cgccggtagt ccacgccacc accagccgac agttgctcta ctccactctg
    16201 gatttgttgt tgctggctct gggcgttgat gccgccgccg tggagtgcga catggttggc
    16261 agctttagcg attttcactg tctgagactc ttctggccgg agggagaagc ctgcctgctg
    16321 cttcagcgct atctcgatcc tgacgatcca gatatgcata gccttatcat gcaccgcctg
    16381 ctgctgggct ggccggaagg tcatctttcg ctggaggcga gctacggccc cgtcatctgg
    16441 tcatccagtc tgtatgtcgc agaccatcag cagaaccccc acagtctctg gcgcagaccg
    16501 gagatcctgc gcgatctgcc gggtctgacg cgcagcgcgg cgcccctgag ctggcgcgac
    16561 tgctgcgaaa ccgtcgggcc ggagggcgtc agctggttgc tacaccagtt gcgcagcaat
    16621 ctggcgggcg aacatccacc agaggcctgc caaagcgtac atcagatcgc gttatcacgt
    16681 ttatggcagc agattttacg taagaccggc aatgcggaga tcagacgtct gacgccgccg
    16741 caccacgacc ggttagccgg tttctacaac gatgatgata aggaggcgct gtgacgcaat
    16801 ctgcaatgtg catcccgctg tggcccgccc ggaacggcaa tactgcgcat ctggtcatgt
    16861 gccctttcgc tggcggcagc agtagcgcgt ttcgccactg gcaagctgag caactggctg
    16921 attgcgcgct ttctctggtg acctggccgg ggcgcgatcg ccttcgccat ctggaaccgc
    16981 tcagaagcat tacacaactg gcggcactgc tggcgaacga gctggaagca tccgtatcgc
    17041 ctgacacgcc gcttttactc gccgggcaca gcatgggggc gcaggtggcg tttgaaacct
    17101 gccgacttct ggagcaacgg gggcttgcgc cacaaggact gattatttcc gggtgccatg
    17161 ccccgcatct gcattctgaa cgccagctca gccatcgcga tgatgccgac tttatcgctg
    17221 agctgataga cattggcgga tgttctcctg aactgcggga aaaccaggaa ttaatgtcgc
    17281 tgtttcttcc tcttctgcgc gctgattttt acgccaccga gagctatcac tacgactcgc
    17341 ccgacgtctg tccgccgctg cgcacgcctg cgctgttatt gtgcggcagc cacgatcgcg
    17401 aagcctcctg gcagcaggtc gatgcctggc gtcagtggct gagccacgtt acaggcccgg
    17461 tggtgattga cggcgatcac ttctatccca ttcaacaagc ccggtccttt tttacgcaga
    17521 ttgtccgcca ttttccccac gcattttctg caatgaccgc gtggcaaaaa cagcccagta
    17581 cttcagaaag gtgacgcatg aattcttcct ttgaatctct gattgaacag tatcccttac
    17641 ccattgccga acagttgcgc cactgggcgg cccgttatgc ctcgcgaatt gccgtcgttg
    17701 atgcaaaggg gtcgttaacc tacagcgcgc ttgatgcaca agttgacgaa cttgccgcag
    17761 gtctgtcatc actgggtttg cgttcggggg agcatgtaat tgtgcagctt cccaacgaca
    17821 acgcgtttgt taccctgctg ttcgccttgt taagactggg cgttatcccc gtgctggcga
    17881 tgccctcgca acgggcgctg gatatcgacg cgctgattga gctggcgcaa cccgtcgctt
    17941 acgttattca cggggaaaac cacgcagagc tggcccgaca gatggcacac aaacacgcct
    18001 gcttgcgtca tgttctggtc gctggagaga ccatgagcga cgattttacg ccgctcttct
    18061 cccttcacgg tgagcgacag cctgatgttt ccaccaccgc gttgttgttg ctctcaggcg
    18121 gcacaaccgg cacgcccaaa ctcatcccgc gccgacatgc cgactatagc tataacttca
    18181 gcgcttctgc tgaactgtgc ggcatcagcc aacagagcgt gtatctcgcc gtcctcccgg
    18241 tggcgcataa ctttccgctg gcctgccccg gtattctggg aacgcttgcc tgcggcggaa
    18301 aagtggtgct gaccgacagc gccagctgtg atgaggtgat gcctttaatc gcgcaggaaa
    18361 gagtgactca cgtcgccctg gttccggcgc tggcgcaatt atgggtgcag gccagggagt
    18421 gggaagacag cgacctttcg tcgctgcgcg tcattcaggc aggcggcgcc cggctcgacc
    18481 cgacgcttgc tgagcaggtt atcgccacct ttgactgtac cctgcaacag gttttcggta
    18541 tggcggaagg cctgctctgt tttacccggc tggacgatcc gcatgccacc attctccaca
    18601 gccaggggcg cccgttgtcc cctctggatg aaatctgcat cgttgatcaa gacgagaacg
    18661 acgtcgcgcc gggcgaaacc gggcaattgt taacgcgcgg cccttatacc atttcgggct
    18721 attaccacgc ccctgcccac aacgcgcagg cctttaccgc gcaagggttt taccgcacag
    18781 gcgacaatgt caggctggat gaggtgggga acctgcacgt tgagggacgc ataaaagagc
    18841 agatcaaccg cgccggagaa aaaatagccg cggctgaagt ggaatcggca ctgctgcgtt
    18901 tagcggaagt gcaagattgc gcggtggtcg ccgcgccgga cacgctgctt ggcgagcgga
    18961 tttgcgcgtt tatcatcgcg cagcaggtgc caactgacta tcagcagttg cgtcaacaac
    19021 tgacccgtat ggggctcagc gcgtggaaaa ttcctgacca aatcgagttt ctggaccact
    19081 ggccgctcac cgccgtcggc aagatagaca aaaaacgcct gacggctctc gccgtcgacc
    19141 gttatcgcca ttctgcccaa taagcgcaaa ccgacccgaa acaggttgaa ataaacccgt
    19201 ttcgggtagc accactatta gaaatagtta tcattttcaa ttcaccattg tcggtatttt
    19261 tggcgtttcg ccgtcttaca gggactcaca acaatgaaaa tgacacggct ttatcctctg
    19321 gccttggggg gattattgct ccccgccatt gctaatgccc agacttcaca gcaagacgaa
    19381 agcacgctgg tggttaccgc cagtaaacaa tcttcccgct cggcatcagc caacaacgtc
    19441 tcgtctactg ttgtcagcgc gccggaatta agcgacgccg gcgtcaccgc cagcgacaaa
    19501 ctccccagag tcttgcccgg gctcaatatt gaaaatagcg gcaacatgct tttttcgacg
    19561 atctcgctac gcggcgtctc ttcagcgcag gacttctata accccgccgt caccctgtat
    19621 gtcgatggcg tccctcagct ttccaccaac accatccagg cgcttaccga tgtgcaaagc
    19681 gtggagttgc tgcgaggccc acagggaacg ttatatggca aaagcgctca gggcgggatc
    19741 atcaacatcg tcacccagca gccggacagc acgccgcgcg gctatattga aggcggcgtc
    19801 agtagccgcg acagttatcg aagtaagttc aacctgagcg gccccattga ggatggcctg
    19861 ctgtacggca gcgtcaccct gttacgccag gttgatgacg gcgacatgat taaccccgcg
    19921 acgggaagcg atgacttagg cggcacccgc gccagcatag ggaatgtgaa actgcgtctg
    19981 gcgccggacg atcagccctg ggaaatgggc tttgccgcct cacgcgaatg tactcgcgcc
    20041 acccaggatg cctatgtggg atggaatgat attaagggcc gtaagctgtc gatcagcgat
    20101 ggttcaccag acccgtacat gcggcgctgc actgacagcc aaaccctgag tgggaaatac
    20161 accaccgatg actgggtttt caacctgatc agcgcctggc agcagcagca ttattcgcgc
    20221 accttccctt ccggttcgtt aatcgtcaat atgcctcagc gctggaatca ggatgtgcag
    20281 gagctgcgcg ccgcaaccct gggcgatgcg cgtaccgttg atatggtgtt tgggctgtac
    20341 cggcagaaca cccgcgagaa gttaaattca gcctacgaca tgccgacaat gccttattta
    20401 agcagtaccg gctataccac cgctgaaacg ctggccgcat acagtgacct gacctggcat
    20461 ttaaccgatc gttttgatat cggcggcggc gtgcgcttct cgcatgataa atccagtaca
    20521 caatatcacg gcagcatgct cggcaacccg tttggcgacc agggtaagag caatgacgat
    20581 caggtgctcg ggcagctatc cgcaggctat atgctgaccg atgactggag agtgtatacc
    20641 cgtgtagccc agggatataa accttccggg tacaacatcg tgcctactgc gggtcttgat
    20701 gccaaaccgt tcgtcgccga gaaatccatc aactatgaac ttggcacccg ctacgaaacc
    20761 gctgacgtca cgctgcaagc cgcgacgttt tatacccaca ccaaagacat gcagctttac
    20821 tctggcccgg acgggatgca gacattaagc aatgcgggta aagccgacgc caccggcgtt
    20881 gagcttgaag cgaagtggcg gtttgcgcca ggctggtcat gggatatcaa tggcaacgtg
    20941 atccgttccg aattcaccaa tgacagtgag ttgtatcacg gtaaccgggt gccgttcgta
    21001 ccacgttatg gcgcgggaag cagcgtgaac ggcgtgattg atacgcgcta tggcgcactg
    21061 atgccccgac tggcggttaa tctggtcggg ccgcattatt tcgatggcga caaccagttg
    21121 cggcaaggca cctatgccac cctggacagc agcctgggct ggcaggcgac tgaacggatg
    21181 aacatttccg tctatgtcga taacctgttc gaccgtcgtt accgtaccta tggctacatg
    21241 aacggcagca gcgccgtcgc gcaggtcaat atgggtcgca ccgtcggtat caatacgcga
    21301 attgatttct tctgattatt gtaa
//
LOCUS       DAZGLJ010000062        21266 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000062 DAZGLJ010000000
VERSION     DAZGLJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.062"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(318..719)
                     /gene="nikR"
                     /locus_tag="ACHK49_002068"
     CDS             complement(318..719)
                     /gene="nikR"
                     /locus_tag="ACHK49_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO9262715.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(725..1531)
                     /gene="nikE"
                     /locus_tag="ACHK49_002069"
     CDS             complement(725..1531)
                     /gene="nikE"
                     /locus_tag="ACHK49_002069"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO9262716.1"
                     /translation="MTLLNISGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(1528..2292)
                     /gene="nikD"
                     /locus_tag="ACHK49_002070"
     CDS             complement(1528..2292)
                     /gene="nikD"
                     /locus_tag="ACHK49_002070"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO9262717.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2292..3125)
                     /gene="nikC"
                     /locus_tag="ACHK49_002071"
     CDS             complement(2292..3125)
                     /gene="nikC"
                     /locus_tag="ACHK49_002071"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO9262718.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMTATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3122..4066)
                     /gene="nikB"
                     /locus_tag="ACHK49_002072"
     CDS             complement(3122..4066)
                     /gene="nikB"
                     /locus_tag="ACHK49_002072"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO9262719.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4066..5640)
                     /gene="nikA"
                     /locus_tag="ACHK49_002073"
     CDS             complement(4066..5640)
                     /gene="nikA"
                     /locus_tag="ACHK49_002073"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9262720.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(5751..6338)
                     /gene="acpT"
                     /locus_tag="ACHK49_002074"
     CDS             complement(5751..6338)
                     /gene="acpT"
                     /locus_tag="ACHK49_002074"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO9262721.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWH
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6393..7442)
                     /locus_tag="ACHK49_002075"
     CDS             complement(6393..7442)
                     /locus_tag="ACHK49_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO9262722.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7574..8791
                     /locus_tag="ACHK49_002076"
     CDS             7574..8791
                     /locus_tag="ACHK49_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9262723.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVTIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(8795..9352)
                     /gene="dcrB"
                     /locus_tag="ACHK49_002077"
     CDS             complement(8795..9352)
                     /gene="dcrB"
                     /locus_tag="ACHK49_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO9262724.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9425..10090)
                     /locus_tag="ACHK49_002078"
     CDS             complement(9425..10090)
                     /locus_tag="ACHK49_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO9262725.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10311..10556
                     /gene="tusA"
                     /locus_tag="ACHK49_002079"
     CDS             10311..10556
                     /gene="tusA"
                     /locus_tag="ACHK49_002079"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO9262726.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(10658..12856)
                     /gene="zntA"
                     /locus_tag="ACHK49_002080"
     CDS             complement(10658..12856)
                     /gene="zntA"
                     /locus_tag="ACHK49_002080"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO9262727.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQSADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEEKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(12930..13556)
                     /locus_tag="ACHK49_002081"
     CDS             complement(12930..13556)
                     /locus_tag="ACHK49_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO9262728.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            13697..14056
                     /locus_tag="ACHK49_002082"
     CDS             13697..14056
                     /locus_tag="ACHK49_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO9262729.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14059..14328)
                     /locus_tag="ACHK49_002083"
     CDS             complement(14059..14328)
                     /locus_tag="ACHK49_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO9262730.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14318..14914)
                     /gene="rsmD"
                     /locus_tag="ACHK49_002084"
     CDS             complement(14318..14914)
                     /gene="rsmD"
                     /locus_tag="ACHK49_002084"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO9262731.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15064..16560
                     /gene="ftsY"
                     /locus_tag="ACHK49_002085"
     CDS             15064..16560
                     /gene="ftsY"
                     /locus_tag="ACHK49_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO9262732.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVAEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16563..17231
                     /gene="ftsE"
                     /locus_tag="ACHK49_002086"
     CDS             16563..17231
                     /gene="ftsE"
                     /locus_tag="ACHK49_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO9262733.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17224..18282
                     /gene="ftsX"
                     /locus_tag="ACHK49_002087"
     CDS             17224..18282
                     /gene="ftsX"
                     /locus_tag="ACHK49_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO9262734.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILMLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18527..19381
                     /gene="rpoH"
                     /locus_tag="ACHK49_002088"
     CDS             18527..19381
                     /gene="rpoH"
                     /locus_tag="ACHK49_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO9262735.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19652..20755
                     /gene="livJ"
                     /locus_tag="ACHK49_002089"
     CDS             19652..20755
                     /gene="livJ"
                     /locus_tag="ACHK49_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO9262736.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(20954..>21266)
                     /gene="panM"
                     /locus_tag="ACHK49_002090"
     CDS             complement(20954..>21266)
                     /gene="panM"
                     /locus_tag="ACHK49_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO9262737.1"
                     /translation="EYSPSSLQVDDNHRIYAARFNERLLAAVRVTLSGTEGALDSLRV
                     REVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGLEDRGVMTAFMQALGFTAQQGGWEK
                     R"
BASE COUNT         5058 a         5616 c         5884 g         4708 t
ORIGIN      
        1 acaccgaaca ggccacgccg gtgggggcac caatacgtac cccggctgaa ccctgaacaa
       61 tgctaccgcc atactgcgtc atgtcaccct gacgcgccgc cggttttccg ctcatcgtaa
      121 aatccttttc tattatttta atgacagtat cttcatacga aaaagcgaag aaaaacttgt
      181 ggtcacaatg aattgaagat ttttaggaat atatttagaa cgttacaaaa gagaaggttc
      241 ggatagggta tttagagagg aatcaggtgt gctagtggcc ctttgttgat cgttcaccaa
      301 aggacccaat aatagactca atcttccttc ggcaagcact gcaaatgccc gtgccgcacg
      361 ccgcgctggg cgataacgtc atcggcaaaa tgctgcacgt cgcccatatc acctttcagc
      421 acggcgattt ccagacagtc gtcgtgattg atatgcacat gcagcgtggc gacggagaga
      481 tcgtggtgat ggtgctgggt ggagacaatg cggctggcta agtcgcgttt ttcgtgttca
      541 tacacatagg agagcaccgc gaaaccttgc gtgccgtgct gctgagtggc ctcctgggcc
      601 agagcgctac gcagaatatc gcggatagct tcggaacgat tgttataacc acgacgctgg
      661 ctcaggctgt ccagcgtctc cagtaaatcg tcatcaagcg tgatggtgac tcgttgcatt
      721 tgcgttaaac cttttctgtg gtgcgacggc gcacggggaa tgcgggtaat accgcgtttt
      781 gtagcacacg tccggcgtca gaggaaaagg ttaatttatc tcccaccacc tgggtttcga
      841 cgatttgtcc gttgtccatc accatcaccc gctgacagaa gcgttccacc aggcgtaagt
      901 cgtgggtgat gaacaggcag gcggtgccaa actgttgttg tagctttttc agcaggcgaa
      961 tgacgcccgc ctgtaacacg agatcaaggt tagaaacggc ttcatccaga atcagtagtt
     1021 tcggttcgac cgccagcgcg cgagccaggc agacgcgctg gagctggccg ccgcttaact
     1081 gcggtgggcg tttgtcgaga acgctgtcat cgagatcgac cgccttcagc atttcgctgg
     1141 cgcgcgccag ttgttcggat tttttcagtg atagcaggtg gcgcatcggt tcacgcagga
     1201 tctcgcgcac ggttttgcgc ggattcacgg cgctgatgga gtcctgaaat accatctgaa
     1261 tatcgcggcg gaacgcttta cgctgggcgc gattgagttt cgccagcggt tcgccacgcc
     1321 agctaatatt cccctgcgcg ggcgattcta aacccaccag caaccgcgcg agggtacttt
     1381 tcccgcagcc gctgcgcccc agcagggcga cggtttcgcc gcttttcagg gtcagggaaa
     1441 cgttattcag caccgcctga tgttgatgtt ttccgttaaa tccaccgtgc gcatagtgat
     1501 gggaaaggcc ggagatgtta agtaaagtca tgatgccagc tccataccgt agagggcgag
     1561 atgagcggaa accaggctgc gcgtcactgt atgtttgggg gcgttaaaca gcgtttctac
     1621 atcgccctgt tcgacaatct taccgtcaga catcaccgcc acgtcatccg ccagacgcgc
     1681 cacaacgccc atatcatggg tcaccagcag cattcccggc gcttgttttt gcataatgct
     1741 ttccagcaga tcgaggatgc gcgcctgtgc taccacgtcg aggtcggtgg tcggttcatc
     1801 ggcgatgata aacggtgatt cacacagcac cgccatcgca atcatcatgc gctgcaacat
     1861 gccgccgctc atctcgaacg ggtacagctt cagcacgcgc gcggcgtttt ccagccccac
     1921 cgcttctatg gcagcggtaa gcgtagcgtc atcggcgggt ttccctaacg ccaggcaggt
     1981 ttcccgcgcg tgggtgtgca tggtgtgcag tggattaaag gcgctgcgcg ggttctgcat
     2041 gatggtggca attttgatgc cgcgcagggc gcaaggcgaa accggtttgc catcggctaa
     2101 aatttccccc gccgtctggc gaacgccagc gggcaaaatg cccagcgtcg cggcgcaggt
     2161 taatgatttc ccgctgccgc taccgccgac taacgccagc acgcgcccgc gttgcagggt
     2221 taacgataca ccgtgtacca gcggctgcgc ggcctgtagc gcgatattac gtagttcaat
     2281 ctgttgcggc attagtgtgc gtgctccgtc accagatgag ggtccagatg atcgcgcagt
     2341 gcgtcaccca ccaggttaaa ggccatcacg ctgataaaca gcgccagccc cggccagaac
     2401 atttgcagcg gctgggtcca gatatactgg cgcgcgtcgt taatcatcac gccccattcg
     2461 gcggtcggcg cggtcacacc gaggccaagg aaagacatcc ccgcgacgtg cagcatcata
     2521 tggccgatat ccagcgttgc cagcaccagc agcgaaggga tcaccgcgcc tgccagatga
     2581 tcgacaaaca cccgcacatg gcccgcaccg gaaagccgtg acgccagcac aaactcgcgt
     2641 tggcgtagtg aaatcaccag gctgcgcacc atgcgtgcat accacgccca gtgcgacagg
     2701 gcgatggcga taattacgtt ggtcagcccg gtgccgagca cgccaaccat aaagaacgac
     2761 agaatcgagg tcgggaaggt cataaacata tcggcgacgc gcatggtggc ctgatcaacg
     2821 cgcccgccaa tcaacccggc gctgccgcca ataaccagcc ctaatgtcag caccagcagc
     2881 aggcaggcca ttaccgaacc gagcgacacg cgggtcgctg tcatcagccg cgagaaaata
     2941 tcgcgaccta agtgatcggt gcccagccag tgctgcgcat ccggcgaaag caggcgcgac
     3001 ggcaaatcaa tcgcctgggg gtcatacggc agccaccact ggctggtgag cgcaatcagc
     3061 gccagcaggg cgataatgat cagcgccagg cgtaccgacc agcgggaaga gaggaaaaag
     3121 ttcacgcgtg cgctccttca tgacgacgaa tgcgcgggtc cagcgcggcg ttgagcaaat
     3181 cgacaatcaa attacagacc acaaaaacca ccaccatcat cagcgtaaag cactggatca
     3241 ccggatagtc acggttaaaa atcgccgaca ccgcatagcg cccgacgccc ggccaggcaa
     3301 agatgttttc gataatcatc gtcccgccaa tcagttcgcc gatatgcatc cccacggcgg
     3361 tgatcatcgg cagcgaggca ttgcgcagga tgtggcgacg ttcggtctgt ttgtcgttca
     3421 ggccgcgcag acgcgcccag gtgacgtgac gctgaccggc gacgtccagc atactggcgc
     3481 gcagtaaacg cgcgttaatc gccagcgaca taaaggcaat ggaaaccgca ggcaaaatga
     3541 tgtgctgcca gccgccgtaa cccatcgcgg gtagccattg cagatacacc gaaaacgcca
     3601 tcaccagtaa aaacgccagc cagaagttag gcatcgacac gccaagaaac gcgatgaaac
     3661 gtacggcgaa atccggcaga cggtcgcgat ggcgcgccgc ccagataccg agcggtacgg
     3721 aagtgagcag aattaatacc agcgccgcac ctgcaagttc cagcgtggcg ggcaggaagt
     3781 tcagcatatc gtccagtacc gggcgttggc tggcgaatga gataccaaag tcaagatgca
     3841 gcgccttcca caaccaggta ccgtactgga cgtacagcgg ctgatccagc ccgagcatgg
     3901 tgcgggtaga ggccagcatc tccggcgtcg gcggcaggtt agataaacgc agataatcga
     3961 gcgccgggtc gccggtaccg aggcgcagca tcagaaaaat gatcaccgag gcggcgagca
     4021 ccatcgggat cagcagcaga aagcggcgta atacgtaacg caacattaag gtttcaccgg
     4081 tttaatctgt tcgaacggaa tttcggtggc gatcggcgcg taggggatgt tacccagctc
     4141 cggttttgat accaccatca ttgagatgta actgataggc agataaaccg cctcgtcatg
     4201 cagacgggtc agaatgtcgc gatacagcgc ctgacgttgc gtttcgtcat gggtcgccag
     4261 cacttcgccg atctctttat caatcagcgg tttgtcggct aatccttgct gtgcctggaa
     4321 gtcagcgtgt gacggtacgc gcattgaact gaggaaggcg tgtggatcat atggcgcgcc
     4381 ccaggtgcgg tggaaaatca tgccaaaacg accgtcgcgc tgacgagcat agatactgct
     4441 ctcttcttcg ccaatcagcg agacatctgc gccaatctgg cgcatatcag cctgaatgat
     4501 ttccgccatc gatttgctta acgcatcggt gccgatgaac gacagttcaa tgcgcagcgg
     4561 ctgaccattt ttctcgcgga tgtctttgcc cgcaggcagc gtccaaccgg ctttttccag
     4621 caacgctttc gctttctgcg gatcgtactg gcgcggtttc aggccgaggt tggcgtaggg
     4681 cacagaaggg gcaaacaggg tgtcggcgac ctgctgggtg ccatacaacg cgttatcaat
     4741 cagcgatttt ttgtttaccg cgtaattaag agcttcacgt actgccagct cgttggtggg
     4801 ggctttggcg gtattgagcg ccagcatcac ggtttcgatc ggctgtgaca gttgggtgtg
     4861 gtaagccgga ttctggctaa agcgggcgaa ggtatcgagc ggtaataacc cttcgtttcc
     4921 gtacagcagg tcgatatcgc cagtttcaaa cgccaccgcg cgggtagtcg ggtccgggat
     4981 gacgttaaag gtgatctttt taatcgctgg cttttcgccc cagtagtttt cgttacggac
     5041 gaagacatcg tactgattca gtttcgattc ctgcaaaatc cacggtccgg tgccaatcgg
     5101 cgctttaatt ccgttcatgg tttcatggtt tttaaactgc gagggagcga taaaacggaa
     5161 aggacggggc agggccagtt cttgcaggaa aggatagtag gcgcttttca gggtaatttg
     5221 cagctctgtt ttactgagtg ctttaacatc aacaatctgg tttgccagct ccagccaggc
     5281 gtgacgttga cggttatcga gcactgcgcg gaagttttct gccgccgcct cggcatcgaa
     5341 cggttcaccg ttggagaatt tcacgtcatc acgcagggtg aaggtccagg ttttaccatc
     5401 ttctgaatga gtccagcttt ttgccagcca cgggatcacc gaaccgtctg cctgatattt
     5461 caccaatggt tcataaacca tgctctgggc gaacatctgg ttaggcgtgt aaaggtgcgg
     5521 gtttagtggc ccgacattca ccggccaggc ggtggtgatt tcatctggtg cagcggcatg
     5581 gacgataaaa gacgcacaag ccagcagcgc aaatagagtg cggcggagtg tggagagcat
     5641 ggtaacccca atggattaaa atagatggcg gaaataagta tgacgatttt aagtattcgt
     5701 catactgatt acctgttctg gatcaataga tgggcagtcg ggtgggcgga tcagttaact
     5761 gaatcgatcc attgcacact gtcggcggtg agcgtaaagg gagtaggggt gcagatcgcc
     5821 aggcttaaat tttcaagctg acaatggctg actgacagcg aggagtgata ggtgctgtct
     5881 acgctgacga tttgccaggc gctgccgcca cgctgtttaa cgatggcttc tttgcgcgtc
     5941 cagatgcgcc agaacatttc cagttgctga tcaggatgca cggcgtccat ctcagcgtgt
     6001 tccccgaggc tgaatacggc gttcgccagc cagtgccagt tggcgcgcgg gcgaatcact
     6061 tcgatatcgc agccgacttc gccttcatca ctcaacagca gggcgatatc gtcaccgcta
     6121 tggcttaagt tgaaccatag cggcatttcc ggcgcaaatg caggtttgcc ttgttcgcca
     6181 tagatgatct ccggtagcgg ggaaagcgtg tgcgaaagca atgcacgccc cgccagccag
     6241 cgttcgcgtc gtggaccttg cggtgcttgc tcgcgtaaac ccggtggcag tggagctgcg
     6301 cttaaggtcg aaactttccc cagaactatc cgatacatat cagggccaac gtttaatgga
     6361 aaatgaaagt gcgtatcgta tcacttgtcg cctcatcccg gtaaccgact tttcggtctg
     6421 cctggcccca gtaaaatcgc cagtttgcta ccgcctttgg tggtttccat ccagatttta
     6481 cacacactgg ttaatggcac cgaaagtagc atccctaccg ggccgagcag ccatccccaa
     6541 attaacaatg aaagaaatac caccatggtg gacatcccca ggcgatggcc catcatccgt
     6601 ggttctaaaa tattgccgat gaccatatgg accactaaaa acaatgcgcc gaccagaata
     6661 cattcgtaaa caccattaaa cagcagcacc tgaatcattg gcggtacggc ggaaattacc
     6721 gcgccgatat tgggcacgta gttgagcaaa aacgccagta ctgcccacat cagcgcaaac
     6781 tgcaccccca tcagctccag ccccagccag acgatgacac ctgtccataa actgagtagc
     6841 gtcttcaatg caagatagtg cgaaacgcct ttaagtgcgc ggtgtaatcc tgcgatgtga
     6901 atctgtgggt tattcagcgc aaaacgcatt ttgtaaggga cgtggcgcac ttcaaacagc
     6961 ataaaaacta cggtcatcac cagcaaaagc acgctcgcca ttgccccgga aagcccggtc
     7021 attagcgctg tggtgaaggt aaccactttt tccgagtcca tccgctgcag cattcgctcc
     7081 ggcgacatat gcaaattaag aaaaggcaac atctcctgca atttaaaaag tttgcgcgtc
     7141 aactccttat taaacttcgg cagcatagag ataaattcgt taaacgatgc cgccagtacg
     7201 ccgaccagcg cagttagtgc gatcagcatc accaccacca caatcgtaat ggcaatgggg
     7261 cgttgtactc cccgacgaat aaaccaggtg acgagcgggt tgaggacgat ggcaaaaaac
     7321 agcgccagta acagctgcac aatgatatct gccgctgcgt gaatgcccgc gaggatcact
     7381 accagcgagg ccagcttgag cagaatgtgc atgcccgttt tatcgggttg aggggtttcc
     7441 attggggctt ccttgtgact ttttgtatta agtgtagcgg gagtcacgcc agcaatattc
     7501 tgattcttac tgctgatttt cacgtcagca cgtggtaaaa atgaaacact gttgtaaaaa
     7561 tgtggtgatc ctcatgcccg aacccgtagc cgaacccgcg ctaaacggat tgcgcctgaa
     7621 tttgcgcatt gtctccattg tcatgtttaa cttcgccagc tacctcacca tcgggttgcc
     7681 gctcgctgta ttaccgggct atgtccatga tgtgatgggc tttagcgctt tctgggcagg
     7741 attggttatc agcctgcaat atttcgccac cttgctgagc cgtcctcatg ccggacgtta
     7801 cgccgatttg ctgggcccca aaaagattgt cgtcttcggt ttatgcggct gctttttgag
     7861 cggtctggga tatctgacgg caggattaac cgccagtctg cccgtcatca gcctgttatt
     7921 actttgcctg ggacgcgtga tccttgggat tgggcaaagt tttgccggaa cgggatcgac
     7981 cctgtggggc gttggcgtgg ttggctcgct gcatatcggg cgagtgattt cgtggaacgg
     8041 cattgtcact tacggggcga tggcgatggg tgcgccgtta ggcgtcgtgt tttatcactg
     8101 gggcggcttg caggcgttag cgttaatcat tatgggcgtg gcgctggtgg ccattttgtt
     8161 ggcgatcccg cgtccgacgg taaaagccag taaaggcaaa ccgctgccgt ttcgcgcggt
     8221 gcttgggcgc gtctggctgt acggtatggc gctggcactg gcttccgccg gatttggcgt
     8281 catcgccacc tttatcacgc tgttttatga cgctaaaggt tgggacggtg cggctttcgc
     8341 gctgacgctg tttagctgtg cgtttgtcgg tacgcgtttg ttattcccta acggcattaa
     8401 ccgtatcggt ggcttaaacg tagcgatgat ttgctttagc gttgagataa tcggcctgct
     8461 actggttggc gtggcgacta tgccgtggat ggcgaaaatc ggcgtcttac tggcgggggc
     8521 cgggttttcg ctggtgttcc cggcattggg tgtagtggcg gtaaaagcgg ttccgcagca
     8581 aaatcagggg gcggcgctgg caacttacac cgtatttatg gatttatcgc ttggcgtgac
     8641 tggaccactg gctgggctgg tgatgagctg ggcgggcgta ccggtgattt atctggcggc
     8701 ggcgggactg gtcacaatcg cgttattact gacgtggcga ttaaaaaaac ggcctccgga
     8761 acacgtccct gaggccgcct catcatctta aaacttactg aataaccagc gtattaatga
     8821 tgttttctgc ggtggtctgc gctttttgct gatcgtcagc tggcagagta atttgcatgg
     8881 tcagcagttg attacccacg ttacccagaa taacggaaga gtacgccgtc tggcctttcg
     8941 cggagataat actgtctaac tgctgcattt tgtgaccttt cagctcaatg gctttattgg
     9001 ttaccacttg cagctgcgga tcgcggctac gttgctgatc ttccagacgc ttcgccagca
     9061 ccgccagatc ttcttttgga tcatcgccca tgatgacgat gactgctttc tgcccggtgg
     9121 cgtcggacca gacatgcatg ttattggcct gcgttcccag cttaccgctc tggtcggtca
     9181 tatccgctgg cagcgagaaa cttaacttgc catcaagcag gttgacggga ttcccggtag
     9241 cgttactttc cgcgaccgaa ccctgcgccg tagcgttagt gtctttatca tcacaggccg
     9301 caagccccat aaccagcagg ccaattccga catatttaac cagattgcgc attgacttct
     9361 tcctttcgat aaacggccat aacggctcat tcatccatct tatcacaact ctgataacga
     9421 acctttaact cgcctgcaaa gcgttgattt cggatttatc tgccagtctt ttcaacagca
     9481 tattgagtaa tacgccatac attggcagga agaaaacgat actgattaac actttgaaac
     9541 agtaatcgac cagcgcgatt tccatccagt gttcagccat aaaggcatcc gggctacgcc
     9601 agaaggcaat gaagaaaaag gccagcgtgt cgctgacgtt accgaacagt gtggacgctg
     9661 tcggtgccag ccaccagcgg cgactttgac gcaggcggtt aaaaacgtgc acgtcgagga
     9721 tttgccccag cgcgtaggcc atgaaactgg cggtggcgat acgggcgaca aacaggttga
     9781 agtgggcgag tgcgccgaat ccctgccatg aacccatata gaacagcgac gagatgacat
     9841 aggagattaa taacgcaggg atcattaccg cgaagataat gcgtcgggcc agcggtgcgc
     9901 caaaaatacg cacggtcagg tcggtagcaa gaaaaataaa cggaaagcta aacgcgcccc
     9961 aggtggtatg gaaaccaaaa atggagacgg gaagctgaac cagatagtta ctggaggtga
    10021 tcaccagcag atgaaataac gataaccaga acaacgcctt atagcgttga gtttgcgaga
    10081 aaacgttcat attgtacctt tttgattaac cattggggtg agggaaccca atacgtacga
    10141 cacgtctgtt accttaacat aataacgtgc ttaccctttt ttcgagccgc cgcatgatac
    10201 tgctttacgt tgacaatgca atggttaatt ttcacgcaat cgttaacctg gtttgcttac
    10261 ggactcacag ggcgtaaact agcgccgttt ttttatgtga tgagaagaaa atgaccgatc
    10321 tcttttccag ccctgaccac acactcgacg cacttggcct gcgctgcccg gaacccgtaa
    10381 tgatggtgcg caaaactgtg cgcaatatgc agcccggcga aacgttgctg attatcgccg
    10441 acgatccggc cactacccgc gatattcctg ggttttgtac ctttatggaa cacgaactgg
    10501 ttgcgaaaga gacggatgga ctgccttatc gttatttgat tcgtaaaggc ggttgatggg
    10561 ggctgattgg cttcgatgcc gccttttccc ctcaccctaa ccctctcccc ggaggggcga
    10621 ggggaccgat cgcgctcaat gttgcgatcg gtttgcctta tctcctgcgc aacaatctta
    10681 acgcattcgc tgtcaccagc accgtcgcac ccgtatctgc cagcaccgcc agccatagtc
    10741 cggtcatccc taacagcgtg gtgacgagga agatcccttt cagtcccagc gcaatggtga
    10801 tgttctggcg gatattggcg tgagtggcgc gtgccagttc aatcatttgc accaggccgc
    10861 gcaggtggtt atgggttaat gctgcatcgg cggtttccag cgccacgtct gtgccgctgc
    10921 ccattgcaat cccaatggcg gcagctttca tcgctggcgc gtcgttaata ccatcaccga
    10981 ccatcgccag cggcgcatgt tgattcagct tggtcaccgc tttgactttc tcttccggca
    11041 ataggcccgc tttaaactcc agccccagct ccccggcgat tgccgccgct gcgcgtggat
    11101 tatcgccggt gaggatcact cctttaactc ccagcgcgtt cagttcactg atggcagttg
    11161 cagcgtcggc gcgcagggta tcctgcaatg caatgatacc cagcacgtca tcattacgta
    11221 ctaccagcac aaccgtttgc ccggcgcttt ccagttcatt aatcaaacca gcaaatgcat
    11281 cagcgggatg tttcccggca gcgcaaatca atacgcgctc accgttaacc tgcgcttcaa
    11341 tgccagaccc gaccagcgcc cgctgtgatt cggcggtggg aatggcgagt tcagcaacct
    11401 gtgcttctcg cacgatggcc tgcgccagtg gatgcgtcgc accttgctcg accgccgctg
    11461 ccagtgtcag cagttcagat tcactaatac ccgttgccgg atgaatcgcg gtaacgcgcg
    11521 gtttaccgac ggtcagcgta ccggttttat caaacgccac ctgggtaaca cgacctagtt
    11581 gttccagcgc cgctccgcct ttaattaacg ccccacgacg cgctgctgcc gccagcccgg
    11641 aggtaatcgc cgcgggcgtg gagataacta acgcacacgg gcagccaatc agcagcagcg
    11701 tcaggccttt ataaatccac tcttgccagc tggcggcaaa cagcagcggt ggcaccagcg
    11761 tcaccagcag agcgacggcc ataatcgcgg gcgtatagat acggctgaaa cggtcgataa
    11821 accgctcaat gggtgcgcga cgctcttcgg cttcttcgat cagtttcaga atccggtcaa
    11881 tggcgctggc acccggttct gacagcactt ccagcgttac cagacggtct acgctggtgg
    11941 ctccagcagg gactttatcg cccgttgcgc gctccaccgg aatagattcg ccggtcaggg
    12001 cgctttcatc aaaactggca aacggtgaga gcagtttacc gtcggcaggc aaacgtccac
    12061 ctgcggcgac ttcaatcaca tcgccagggc gcaggctgtt aatcgccacc tcttcccgct
    12121 caccgttacg caggcgcgtg gcggtttctg gtttcagcgc cattaacgcg ctgacccctt
    12181 gacgtgcgcg gctggcggcc cagccttcca gtcgttcgcc aatcaaaaac agcagcaaca
    12241 ccatcgcagc ttcagccgtt gcgccaataa aaagcgcacc aatagcggct acgctcatta
    12301 aggtttcaat ggcgaagtag ctgccggatt tgatcaaccg taatgcctga cgagcaatcg
    12361 ggtacagccc aaccagtgtg gtcgcgataa acgccagttg cccgaacgga tgattgaact
    12421 gctccagacc ccagctgatt gccatcatca cgattagcgt aatcagcggc agattctctt
    12481 tcaggcgtga tgcttgcggt tcgtcggcgg actgttcatc gcgcagggag tagcccgctt
    12541 tttgcaccgc agattcaacc tgggcacgga tgtcattgtc ggcatcaacc accagttttt
    12601 cggtggcgaa caacacctgt acctgattca cgcctgcaag ctggcgcacg gcattttcta
    12661 ccttgcgcgc acaggcggcg cagtccatgc cgctgacttt ccagctatag cgggtgccgg
    12721 agacgttttc agagagagtt ggcgtgctgg aacatgcgcc gtcgcagcaa cagtcgttgg
    12781 cgttctgtac cgtggttagc ggtttgaacg cagcaaattg aggggctttc ttgccgtgat
    12841 tgtcaggagt cgacatggca tcctccggtt aagttttttc tcattaaccg aaggatacac
    12901 tctggagtcg actccagagt caagttttat cagagataca gcgagcggac gatcaggaag
    12961 tgaccggcaa agtagcaggc cgcagcaatc gcgttatccg cacggaagcg tcggcgatag
    13021 tgactaccca gccagacaaa gttactgata aacagcaacg aagcgccgac aaacgcagag
    13081 agcgccggag cggtcggacg gaagaaccac agttcacctg ccagccacac catcaccagc
    13141 gtcatgccga taaacgtgca gataggccaa cgatactctt ccagccgcgt ccagataatc
    13201 gccagtaaca gcgcgcccag aaccagcagc accagtggca gaggccagaa gaaagagagc
    13261 gtcatctgac tggcgaaata gatggtgtac agcaggtgcg agaggaaaaa cgcgccgatg
    13321 gcgtacatca gacgttgacg tggcaacagg gttagcgcat cgcccagcag tgaggcgcac
    13381 agccccgcca gcaccagata gctaatggcg tcgaacatcg gcgcttgcca ggccagtaac
    13441 agcaggagaa gaagggttaa tggtttaaat acccagcgtt gccaggttgg cccacgatac
    13501 gatgcatcca cagatagcca tgcggaaaga cagacagcga taaacgacca aagcatctta
    13561 gttccttgaa ttcgttattt tttcgtaagc ggaatgccta cggtcttgct tccagtttag
    13621 tggcgagggt ggacggatga caacaccata actggcaggg tatgcttaat tccgcatttt
    13681 ccgatgaggg ataaagatga gcaaaccacc tcttttcttt attgttatca ttggcttaat
    13741 catcgtcgcc gcatcgtttc gttttatgca acaacgacgg gaaaaagctg ataatgatat
    13801 ggcaccactc cagcaaaagc tggtgttggt gagcaacaag cgggaaaaac cgattaacga
    13861 tcgccgttcg cgccagcagg aagtgactcc ggcaggcacc agtatgcgct atgaggcaag
    13921 cttcaaaccg caaagcggag gaatggagca gacgtttcgc ctcgacgccc agcagtacca
    13981 cgccctgaca gtgggcgata aaggtacgct gagctataaa ggaacgcgct ttgtcagctt
    14041 tgtaggcgaa caataaggtt attttttaac tttaaatttc ttctgccaga ccagcagttc
    14101 aaacacgccg aaaaggaaaa tccgtacctt ttcggcggtg gtcatctgcg ggccatcttt
    14161 cggtaaagtg gatttcaata gcgccagctg cataccatgc atcagcacgg taaaaatcag
    14221 cgccacgtta acgaaaatat tcagcggacg tgggaaggga tgcaccaggt tgaggattaa
    14281 aaatccccaa acacagagca ttaacaaacg accaatatta atcagcatca ctttctcctt
    14341 gtgcttcgcg ttgatacagc cgataagcca cctgacccgc cactttttcc cgatgtaatg
    14401 accagtttgc cggaacagtg ggcagaccgt tttcgacttc gctttcgaca taaatcaggg
    14461 cttcgtcagc cagccagccg ttatcttcca gtaaatttat cgtctcttct aacaagccac
    14521 ggcggaacgg tggatcgaca aacacgatat tatgcggtgt acctttttgc gccaggaatg
    14581 acatcgcgtt gctgttcacc acgcgtgcat tgcctgcttt tagtgtcgcc agattcttaa
    14641 ttaactgctg agaaaccgcg cgatccatct caatcaacgt tgcccccgca gcatagcgcg
    14701 acaacgcttc cagccccagc gcgccgcttc cggcgaagca atccagacat tgggcgtcaa
    14761 caatgaccgg agccagccag ttaaacaacg tttcgcgtac gcggtcggtg gtggggcgca
    14821 gacctgggct atcaggaacc gggagtttac ggcctcgcca ctgcccgcca ataatgcgga
    14881 tttggccgct gccggaatga ttcggttttt tcatgataaa ttgctcaatc cgccagataa
    14941 cacatacttg caggcggtga tgtgaattaa gttaagtgat agactatttc atcatttttt
    15001 tagctgctat gtacatagcg ttaacgctgt gccatgaagc aacagcgagg agtgtagtcg
    15061 caaatggcga aagaaaaaaa acgtggcttt ttttcctggc tgggctttgg tcaaaaagag
    15121 cagaccccgg aaaaagagac agaagttcag aatgaacaac cggttgtaga agaaatcgtt
    15181 caggcgcaag agcctgtgaa ggcttcggaa caagccgttg aagagcagcc gcaggcgcat
    15241 actgaagccg aggcggaaac ttttgctgcc gacgttgtgg aagtcactga acaggttgct
    15301 gaaagtgaaa aagcgcagcc tgaagcggaa gtcgttgcac agccggaacc ggtcgcagaa
    15361 gaaacgccgg agccagtggc tatcgaacgt gaagagctgc cgttgccaga agacgtcaac
    15421 gccgaagagg tttcgccaga agagtggcag gctgaagcgg aaaccgtaga gattgtcgaa
    15481 gcggcagaag aagaagcggc taaagaagaa atcaccgacg aagagctgga agcacaggcg
    15541 ctggctgccg aagcggcaga agaggcggtg atggtagttc ctccggcaga agaagagcag
    15601 ccggtggaag aaattgccca ggagcaagaa aaaccgacca aagaaggttt cttcgcgcgc
    15661 ctgaaacgca gcctgttaaa aaccaaagaa aatctcggtt ccggatttat cagcctgttc
    15721 cgcggtaaaa aaatcgacga tgatctgttt gaggagctgg aagaacagct gttgatcgcc
    15781 gatgtgggcg tggaaaccac acgtaaaatt atcaccaatc tgacggaagg cgcatcccgc
    15841 aagcagcttc gtgatgccga ggcgctctat ggcctgctga aagaagagat gggcgagatt
    15901 ctggcgaaag tcgatgagcc gctgaatgtt gaaggtaaaa cgccgttcgt gatcctgatg
    15961 gtgggcgtca acggtgtggg taaaactacg acgattggta agctggcacg tcagtttgag
    16021 cagcagggta aatcggtgat gctggcggcg ggcgatactt tccgtgcagc agcggttgaa
    16081 cagcttcagg tctggggtca gcgcaacaat attccggtga ttgcccagca taccggtgcg
    16141 gattccgcct ctgttatctt cgacgccatt caggcagcta aagcgcgtaa tatcgacgtc
    16201 ctgattgccg ataccgccgg acgcctgcag aacaaatcgc acctgatgga agagttgaag
    16261 aaaatcgtcc gcgtgatgaa gaaactcgac gttgaagcgc cgcatgaagt tatgctgact
    16321 attgatgcca gtaccgggca gaatgcggta agccaggcca aactgttcca cgaagccgtt
    16381 ggcttaaccg gcatcacgct aacgaaactg gacggcacgg cgaaaggcgg ggtaattttc
    16441 tcggtggctg accagtttgg tatccctatc cgctacattg gtgtcggcga acgtattgag
    16501 gatttgcgtc cgtttaaggc ggacgacttt atagaggcac tttttgcccg agaggattaa
    16561 caatgattcg ctttgaacat gtcagcaagg cttatctcgg tgggagacag gcgctgcagg
    16621 gcgttacgtt ccatatgcag ccgggtgaga tggcgtttct gaccggtcat tccggcgcag
    16681 ggaaaagtac cctcctgaag ctgatctgtg ggattgagcg gcccagcgcc gggaaaatct
    16741 ggtttagcgg ccatgacatc acgcgtctga aaaaccgtga agttccgttt ctgcgccgcc
    16801 agattggcat gattttccag gatcaccatc tactgatgga ccgtactgtc tacgataacg
    16861 tggcgatccc gctgattatc gctggtgcca gcggtgacga tattcgtcgc cgggtgtcgg
    16921 cggcactgga taaagtcggg ctactggaca aagcgaagaa cttccctatt cagctttccg
    16981 gcggtgaaca acagcgtgtt ggcattgccc gcgcggtggt gaataagccc gcggtactgc
    17041 tggcggacga accgaccggt aacctggacg acgcgttgtc ggaagggatt ctacgtctgt
    17101 ttgaagagtt taaccgcgtt ggggtaaccg tattgatggc aacgcacgac atcaacctga
    17161 tttcacggcg ttcctatcgc atgctcaccc tgagcgatgg tcacttgcat ggaggcgtgg
    17221 gccatgaata agcgcgatgc aatcaatcat attcggcagt ttggcgggcg tcttgatcgc
    17281 ttccgtaaat cggtcggcgg ctctggcgac ggcggtcgta acgccccgaa acgcgcgaaa
    17341 tcctcgccaa aaccggtaaa tcgtaaaacc aacgttttca acgaacaggt gcgctatgcc
    17401 ttccacggcg cattgcagga tctgaaaagc aaaccgttcg ccacgttttt aacggtgatg
    17461 gttatcgcca tctccctgac gctgcccagc gtctgttata tggtgtacaa aaacgttaat
    17521 caggcggcga cgcagtatta tccgtcaccg caaatcactg tttacttgca aaaaacgctg
    17581 gacgatgacg ccgctgcggg cgtggtggca cagttgcagg ccgagcaagg cgtggagaaa
    17641 gtgaactatc tttctcgtga agacgcactg ggcgagttcc gtaactggtc tggttttggt
    17701 ggtgcgctgg atatgctgga agaaaacccg cttccggcag tggcggtggt gatcccgaaa
    17761 ctcgattttc aggggacgga atcactcaat acgctgcgtg atcgtatcac gcagattaac
    17821 ggcattgacg aagtgcggat ggatgacagc tggtttgccc gtctggcggc gttgaccggg
    17881 ctggtcgggc gcgtttcggc gatgatcggc gtgttgatgg tggcggcagt gttcctcgtc
    17941 atcggcaaca gcgtacgtct gagcatcttt gctcgccgtg actccatcaa cgtgcagaaa
    18001 ctgattggtg cgacggatgg tttcatcctt cgcccgttcc tgtatggtgg cgcactgctg
    18061 ggattttctg gcgcattgtt gtcattaatt ttgtcagaaa ttctgatgct gcgattgtca
    18121 tcggcggttg cggaagtggc acaggttttc ggaacgaagt ttgatatcaa tggtttatcg
    18181 ttcgatgaat gcctgctatt gctgctggta tgctcgatga ttggctgggt ggcagcgtgg
    18241 cttgccacgg tacaacattt acgccacttt acgcctgaat aataaaagcg tgttatactc
    18301 tttccctgca atgggttccg tagcagggaa agagatcccg ttgtctcttc ccggtatttc
    18361 atctctatgt cacattttgt gcgtaattta ttcacaagct tgcattgaac ttgtggataa
    18421 aatcacggtc tgataaaaca gtgaatgata acctcgttgc tcttaagctc tggcacagtt
    18481 gttgctacca ctgaagcgcc agaagatatc gattgagagg atttgaatga ctgacaaaat
    18541 gcaaagttta gctttagccc cagttggcaa cctggattcc tacatccggg cagctaacgc
    18601 gtggccgatg ttgtcggctg acgaggagcg ggcgctggct gaaaagctgc attaccatgg
    18661 cgatctggaa gcagctaaaa cgctgatcct gtctcacctg cggtttgttg ttcatattgc
    18721 tcgtaattat gcgggctatg gcctgccaca ggcggatttg attcaggaag gtaacatcgg
    18781 cctgatgaaa gcagtgcgcc gtttcaaccc ggaagtgggt gtgcgcctgg tctccttcgc
    18841 cgttcactgg atcaaagcag agatccacga atacgttctg cgtaactggc gtatcgtcaa
    18901 agttgcgacc accaaagcgc agcgcaaact gttcttcaac ctgcgtaaaa ccaagcagcg
    18961 tctgggctgg tttaaccagg atgaagtcga aatggtggcc cgtgaactgg gcgtaaccag
    19021 caaagacgtt cgtgagatgg aatcacgtat ggcggcacag gacatgacct ttgacctgtc
    19081 ttccgacgac gattccgaca gccagccgat ggcaccggtg ctctatctgc aggataaatc
    19141 atctaacttt gccgacggca tcgaagatga taactgggaa gagcaggcgg caaaccgtct
    19201 gaccgacgcg atgcagggtc tggacgaacg cagccaggac atcatccgtg cgcgctggct
    19261 ggacgaagac aacaagtcca cgttgcagga actggctgac cgttacggtg tttccgctga
    19321 acgtgtgcgc caactggaaa agaacgcgat gaaaaaactt cgcgccgcta ttgaagcgta
    19381 atttccgcta ttaagcagag aaccctggat gagagtccgg ggtttttgtt ttttgggcct
    19441 ctacaataat caattccccc tccggcaaaa cgtcaatccc cacgcagatt gttaataaac
    19501 tgtcaaaata tctattccaa tatcataaaa atggggtatg ttttagcaga gtatgctgct
    19561 aaagcacggg tagtcatgca taaaacgaaa taaagtgctg aatcacaaca ccacaacaca
    19621 cgtaataacc agaagaatgg ggattctcag gatgaatata aagggtaaag cgttactggc
    19681 aggatgtatc gcgctggcat tcagcaatat ggctctggca gaagatatta aagtcgcggt
    19741 cgtgggcgca atgtccggtc cggttgcgca gtacggtgac caggagttta ccggcgcaga
    19801 gcaggcggtt gcggatatca acgctaaagg cggcattaaa ggcaacaaac tgcaaatcgt
    19861 aaaatatgac gatgcctgtg acccgaaaca ggcggttgcg gtggcgaaca aagtcgttaa
    19921 cgacggcatt aaatatgtga ttggtcacct ctgttcttca tcaacgcagc ctgcgtctga
    19981 tatctacgaa gacgaaggca ttttaatgat caccccagcg gcaaccgcgc cggagctgac
    20041 cgcccgtggc tatcagctga tcctgcgcac caccggcctg gactccgacc aggggccgac
    20101 ggcggcgaaa tatattcttg agaaagtgaa accgcagcgt attgctatcg ttcacgacaa
    20161 acagcaatac ggcgaaggtc tggcacgagc ggtgcaggac ggcctgaaga aaggcaatgc
    20221 taacgtggtg ttctttgatg gtatcaccgc cggggaaaaa gatttctcta cgctggtggc
    20281 gcgtctgaag aaagagaata tcgacttcgt ttactacggc ggttaccacc cggaaatggg
    20341 gcaaatcctg cgtcaggcac gcgcggcagg gctgaaaact cagtttatgg ggccggaagg
    20401 tgtggctaac gtttcgctgt ctaacattgc gggcgaatca gcggaagggc tgctggtgac
    20461 caagccgaag aactacgatc aggttccggc aaacaaaccc attgttgacg cgatcaaagc
    20521 gaaaaaacag gacccaagcg gcgcattcgt ttggaccacc tacgccgcgc tgcaatcttt
    20581 gcaggcgggc ctgaatcagt ctgacgatcc ggctgagatc gccaaatacc tgaaagcgaa
    20641 ctccgtggat accgtaatgg gaccgctgac gtgggatgag aaaggcgatc tgaaaggctt
    20701 tgagttcggc gtatttgact ggcacgccaa cggcacggcg accgatgcga agtaatcatt
    20761 aatcggcaac tttgggttgc cgccaaattg ctaatatcga gtacgttgct tcatgccgga
    20821 tgcggcgtaa acgccttatc cggcctacaa gatccaaaga aatcagtaaa ttgcaacaca
    20881 cattgtaggc ctgataagcg tagcgcatca ggcaatttct cgttggtcat cacttttgaa
    20941 atcaacttga cgattaacgc ttctcccagc cgccctgttg cgccgtaaac cccagcgcct
    21001 gcataaacgc cgtcatcaca ccgcgatctt ccaagcctgc atccgccatc caccagcatg
    21061 aaacgccagg attgttacgc aaaacctctt ccagcagata ttgccccaca ccgcgacgtc
    21121 gggtgacttc ccgcacacgc agggaatcca gtgctccctc ggtgccgctt aaggttaccc
    21181 gtacggcagc gagcaggcgc tcgttaaaac gcgcggcgta gatacggtgg ttatcgtcaa
    21241 cctgtaaaga ggaaggggaa tactcc
//
LOCUS       DAZGLJ010000063        21134 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000063 DAZGLJ010000000
VERSION     DAZGLJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.063"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            38..1684
                     /gene="mqo"
                     /locus_tag="ACHK49_002091"
     CDS             38..1684
                     /gene="mqo"
                     /locus_tag="ACHK49_002091"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO9262738.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(1833..2342)
                     /gene="eco"
                     /locus_tag="ACHK49_002092"
     CDS             complement(1833..2342)
                     /gene="eco"
                     /locus_tag="ACHK49_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO9262739.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(2457..2621)
                     /gene="yojO"
                     /locus_tag="ACHK49_002093"
     CDS             complement(2457..2621)
                     /gene="yojO"
                     /locus_tag="ACHK49_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFO9262740.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            2730..3224
                     /gene="napF"
                     /locus_tag="ACHK49_002094"
     CDS             2730..3224
                     /gene="napF"
                     /locus_tag="ACHK49_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO9262741.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            3214..3477
                     /gene="napD"
                     /locus_tag="ACHK49_002095"
     CDS             3214..3477
                     /gene="napD"
                     /locus_tag="ACHK49_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO9262742.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            3474..5960
                     /gene="napA"
                     /locus_tag="ACHK49_002096"
     CDS             3474..5960
                     /gene="napA"
                     /locus_tag="ACHK49_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HFO9262743.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            5967..6662
                     /gene="napG"
                     /locus_tag="ACHK49_002097"
     CDS             5967..6662
                     /gene="napG"
                     /locus_tag="ACHK49_002097"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO9262744.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            6649..7512
                     /gene="napH"
                     /locus_tag="ACHK49_002098"
     CDS             6649..7512
                     /gene="napH"
                     /locus_tag="ACHK49_002098"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO9262745.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGYICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            7509..7958
                     /gene="napB"
                     /locus_tag="ACHK49_002099"
     CDS             7509..7958
                     /gene="napB"
                     /locus_tag="ACHK49_002099"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO9262746.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            7968..8570
                     /gene="napC"
                     /locus_tag="ACHK49_002100"
     CDS             7968..8570
                     /gene="napC"
                     /locus_tag="ACHK49_002100"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO9262747.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            8583..9206
                     /gene="ccmA"
                     /locus_tag="ACHK49_002101"
     CDS             8583..9206
                     /gene="ccmA"
                     /locus_tag="ACHK49_002101"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO9262748.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            9203..9865
                     /gene="ccmB"
                     /locus_tag="ACHK49_002102"
     CDS             9203..9865
                     /gene="ccmB"
                     /locus_tag="ACHK49_002102"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO9262749.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VSGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            9907..10644
                     /gene="ccmC"
                     /locus_tag="ACHK49_002103"
     CDS             9907..10644
                     /gene="ccmC"
                     /locus_tag="ACHK49_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO9262750.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            10641..10850
                     /gene="ccmD"
                     /locus_tag="ACHK49_002104"
     CDS             10641..10850
                     /gene="ccmD"
                     /locus_tag="ACHK49_002104"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO9262751.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            10847..11326
                     /gene="ccmE"
                     /locus_tag="ACHK49_002105"
     CDS             10847..11326
                     /gene="ccmE"
                     /locus_tag="ACHK49_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO9262752.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            11323..13266
                     /gene="ccmF"
                     /locus_tag="ACHK49_002106"
     CDS             11323..13266
                     /gene="ccmF"
                     /locus_tag="ACHK49_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO9262753.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            13263..13820
                     /gene="dsbE"
                     /locus_tag="ACHK49_002107"
     CDS             13263..13820
                     /gene="dsbE"
                     /locus_tag="ACHK49_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO9262754.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            13817..14869
                     /gene="ccmH"
                     /locus_tag="ACHK49_002108"
     CDS             13817..14869
                     /gene="ccmH"
                     /locus_tag="ACHK49_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO9262755.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQENK"
     gene            complement(14904..15551)
                     /gene="narP"
                     /locus_tag="ACHK49_002109"
     CDS             complement(14904..15551)
                     /gene="narP"
                     /locus_tag="ACHK49_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO9262756.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            15871..18462
                     /gene="yejO"
                     /locus_tag="ACHK49_002110"
                     /pseudo
     CDS             15871..18462
                     /gene="yejO"
                     /locus_tag="ACHK49_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(18930..19853)
                     /locus_tag="ACHK49_002111"
     CDS             complement(18930..19853)
                     /locus_tag="ACHK49_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO9262757.1"
                     /translation="MISKNMKGVSAVRAASVLTSCKFSHADAIEMTKKNDPAVSKFLE
                     KSIVAGMTTDNMPSLVAEPIVRELGEYIFSQSVPGKLISSALNLPFGAKLQSISGKGS
                     KWLKEAEEIPVKEGEVNESALKLCKLGAIVMLNNELIKFSTPGTNSAIRDLITSESVK
                     EIDKKFLSSDQEVAGVSPEGVLNGADEAENFIELFKKHIANGNTLATSSLILPVENAL
                     QLTDAEFWQIELLRIPVIVSEQADRMMLIDAQKLIINVEATVITPTDEAVIKTESGTV
                     SLFQNDATAFRAITYCGWQKLDKAVTVLAKS"
     gene            complement(19843..20004)
                     /locus_tag="ACHK49_002112"
     CDS             complement(19843..20004)
                     /locus_tag="ACHK49_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9262758.1"
                     /translation="MSEYITDPIDVLTEIMNDKQQPTEIRLQAAAVLMPYFHETLTPC
                     YQEEEDNDF"
     gene            complement(20016..20504)
                     /locus_tag="ACHK49_002113"
     CDS             complement(20016..20504)
                     /locus_tag="ACHK49_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO9262759.1"
                     /translation="MALTTKKKRFADALSKGTNPTEAAIHAGYSEKTARVKGWQLSND
                     PEVKKYLKAVTSDVTFQVTKPKKVTKKVTEKVTPERASINTIEMMDNGLPDPIKAMGR
                     ILIDNMDTDPKLALDAAFKLAQFTHRKIGSIGKKEVKKLNAANVSSNRFPIPKPPTGL
                     KH"
     gene            complement(20632..20793)
                     /locus_tag="ACHK49_002114"
     CDS             complement(20632..20793)
                     /locus_tag="ACHK49_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein NusG"
                     /protein_id="HFO9262760.1"
                     /translation="MAITEREASIIKAIGEETRDTIAPLMEKIAELETRINKLSDEVE
                     GLISLLGEQ"
     gene            complement(20797..20991)
                     /locus_tag="ACHK49_002115"
     CDS             complement(20797..20991)
                     /locus_tag="ACHK49_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9262761.1"
                     /translation="MEEIKQSATVMIDHEILNELKKLQFDIVRRMNESGIYGRAATPT
                     IGHLARVLLRERLGIATSEA"
BASE COUNT         4772 a         5154 c         5910 g         5298 t
ORIGIN      
        1 cctgcaataa agcgactaaa agtaaggcat taacaagatg aaaaaagtga ctgccatgct
       61 cttctcgatg gccgtggggc ttaatgccgt ttcgatggcg gcaaaagcga aagcgtccga
      121 ggagcaggaa actgatgtac tgttgattgg cggcggcatt atgagcgcca cgttggggac
      181 ctatttacgc gagctggagc ctgaatggtc gatgaccatg gtggagcgcc tggagggtgt
      241 cgcgcaggag agttcgaacg gctggaataa cgccggaacc gggcattctg cactgatgga
      301 actgaactac accccgcaaa acgccgatgg cagcatcagt attgaaaaag cagtcgccat
      361 taacgaagca tttcagattt cccgccagtt ctgggcgcac caggttgagc gcggcgtgct
      421 gcgtactccg cgttcattta tcaataccgt tccgcatatg agctttgtct ggggcgagga
      481 taacgttaat ttcctgcgcg cccgttacgc cgcgttgcaa caaagctcgc tgtttcgcgg
      541 tatgcgttac tctgaagatc acgcgcagat caaagagtgg gcaccgttag tgatggaagg
      601 gcgcgatcca caacagaaag tagcggcaac acgcacagaa attggcactg atgtgaacta
      661 tggcgagatc acccgccagt taattgcttc cttacagaag aaatctaact tctcgctgca
      721 actcagcagc gaagtccgcg ccctaaagcg taatgacgat aacacctgga ccgttaccgt
      781 tgccgatctg aaaaacggca ctgcacagaa catccgcgcc aaatttgtct ttatcggcgc
      841 gggcggtgcg gcgctgaagc tattacagga atcggggatt ccggaagcga aagactacgc
      901 cggtttcccg gtgggcggac agttccttgt ttcggaaaac ccggacgtgg ttaatcacca
      961 tctggcgaag gtttacggta aagcatccgt tggcgcacca ccgatgtcgg ttccgcatat
     1021 cgatacccgc gttctggacg gtaaacgcgt agtgctgttt gggccatttg ccaccttctc
     1081 aaccaaattc ctcaaaaacg gttcattgtg ggatctgatg agttccacca ccacctctaa
     1141 cgtgatgccg atgatgcacg tcgggctgga taatttcgat ctggtgaaat atctggtgag
     1201 tcaggtgatg ttgagtgaag aggatcgttt tgaagcgttg aaagagtact atccgcaggc
     1261 aaaaaaagag gactggcgtt tgtggcaagc ggggcagcgc gtgcagatta tcaagcgtga
     1321 tgccgagaaa ggtggcgtac tgcgtctggg tactgaagtg gtcagtgacc agcaagggac
     1381 cattgccgcg ctgctggggg catcgccagg ggcgtcaacc gccgcgccga ttatgttgga
     1441 tctgctggaa aaagtatttg gcgatcgcgt ttccagcccg caatggcagg ctacgttgaa
     1501 agcgatcgtt ccgtcttatg gacgcaagct gaacggtgat gtagcggcaa cagaacgcga
     1561 gttgcagtac accagcgaag tgctggggct gaaatacgac aagccgcagg cagcagatag
     1621 tacgccaaaa ccgcagttga aaccgcaacc cgttcaaaaa gaagtggcgg atattgcgtt
     1681 gtaatgatgt gccacatccg gcatggtatg ccggatgtgg cgtatgctga taagacgcgc
     1741 cagcgtcgca tcaggcaccg gctcgggcgt tagatgtcgg atgcggcgtg accgccttat
     1801 tcggcctacg gggtgccgca cttcacagca ttttagcgaa ctaccgcgtt gtcaattttc
     1861 tcttccgcct tccagacgcg gtacttcaca tctacattgt ctggcgtata caccacgatc
     1921 ggcagcttgc tgttgtaacg cagcattcca gcatcgccca gatacgcggt gacaaatttc
     1981 ttctctttct tgccatccgg gcaggccatc atcgtggaaa ccggcgagct aactttatca
     2041 aagacataat aatcatagcc ccagccttcc agcgttttgc tttccagctt cccgccgaga
     2101 cgatgcaaat tgcaatcgac ttccagcgtc tgaccgatta acagttctac tttcagggta
     2161 gattcatctt cttgcggggt taactgaatc acctgacgct tcatcccttt ttcagcttgt
     2221 ggataaggcg cgattttttc cagtggctgg acgctttctg ccgcccaggc ggaagtggta
     2281 gcgaaagcgg caaacaatac tgcaggtaga atggtcttca tttttttgct ttccttcttc
     2341 aatttatagg ttttcaccga tgatgtcgct gaaggttaac atattcatca gatttattaa
     2401 tttgctttat gaataactta aagaacattc agacaatttt acctgctctt ttagtgttaa
     2461 attctgatga gggagcaaac cagcagaaat atctctggtg aattaatcgc tatataaata
     2521 tgtttataac catttgaaat gtgagcaaaa gcccgttttt gccacgcttc ccgccactct
     2581 tttgatcctg ctacaggttt taccccgatc ggggtatgca tctttgacac atcctttaat
     2641 atcttagcgg ctataaaaat ggcttattaa ttatgcggtt tatttggtcg ctctcaattt
     2701 tcagagcgcg ttaatgatgg aaggtcaatg tgaagattga tgcatcccgt cggggcatac
     2761 tcactggtcg ctggcgcaaa gccagtaacg gtatccgtcc gccctggtcc ggtgatgaat
     2821 ctcattttct gacccattgc acccgctgcg acgcctgtat taatgcttgt gaaaacaaca
     2881 ttctgcaacg cggcgcgggc ggctatccga gcgttaattt caaaaataac gagtgcagct
     2941 tctgttacgc ctgcgcgcag gcttgccccg aatcgctatt ttctccgcgc cacaccaggg
     3001 cctgggattt gcagttcacc attggggacg cgtgcctggc gtatcagtca gttgaatgcc
     3061 gccgctgtca ggatagctgt gagccaatgg caattatctt tcgcccgacg ctctccggga
     3121 tctaccagcc gcaacttaat agccaacttt gcaacggatg tggagcatgt gcagccagct
     3181 gtccggtatc agccataact gcggagtatc tccatgcaca ctaactggca agtttgcagc
     3241 ctggtcgtgc aggccaaaag cgaacgaatt tcagacatca gcacccaact gaacgccttt
     3301 cccggctgtg aagttgctgt cagcgacgcg ccgagcggtc agttgattgt ggtggtggaa
     3361 gcagaagaca gcgaaacgct gatccaaacc attgagtcag tacgcaacgt agagggcgtg
     3421 ctggcggtgt cgctggttta tcaccagcag gaagagcaag gtgaggaaac accatgaaac
     3481 tcagtcgtcg tagctttatg aaagctaacg ccgttgcggc cgctgcggcg gctgccggtc
     3541 tcagcgtgcc gggcgttgcc cgcgccgttg ttggtcagca ggaagccatt aaatgggata
     3601 aagcgccgtg ccgtttctgc ggtactggtt gcggcgttct ggtcggaacg cagcaggggc
     3661 gtgtggtggc ctgtcagggc gacccggacg caccggttaa ccgtggcctg aactgcatta
     3721 agggctattt cctgcccaag atcatgtacg gtaaagaccg tttgacgcag ccgctgctgc
     3781 gtatgaaaaa cggtaaatat gacaaagaag gcgaatttac cccaatcacc tgggatcagg
     3841 ccttcgatgt gatggaagag aaattcaaaa ccgccctgaa agaaaaagga ccggaatcga
     3901 tcggtatgtt cggttctggt cagtggacta tctgggaagg ttatgccgcg tccaagctgt
     3961 tcaaagcggg cttccgttcg aacaacatcg acccgaacgc acgtcactgt atggcgtcgg
     4021 cagtagttgg ctttatgcgt acctttggta tggatgagcc gatgggctgc tatgacgaca
     4081 tcgagcaggc tgacgcgttt gtgctgtggg gcgctaacat ggcggagatg cacccgatcc
     4141 tctggtcacg catcactaac cgtcgtctct ctaaccagaa cgtcaccgtg gcggtgcttt
     4201 ctacctacca gcatcgtagc ttcgagctgg cggataacgg catcatcttt acgccacagt
     4261 ctgacctggt gatcctgaac tacatcgcca actatatcat tcaaaacaat gcgataaatc
     4321 aggacttctt cagcaagcac gttaacctgc gcaaaggggc gacggacatc ggctacggtt
     4381 tacgtccgac ccatccgctg gaaaaagcag cgaagaatcc gggttctgac gcctccgaac
     4441 cgatgagctt tgaagattac aaagccttcg ttgccgagta tacgctggaa aaaactgccg
     4501 aaatgaccgg cgtgccgaaa gaccagttag aacaactggc gcagctgtat gccgatccga
     4561 acaagaaagt catctcctac tggacgatgg gcttcaacca gcatactcgt ggcgtgtggg
     4621 ccaacaacct ggtctacaac ctgcacctgc tgaccggcaa aatttcccag ccgggttgcg
     4681 gtccgttctc cctgaccggg cagccttccg cgtgtggtac tgcgcgtgaa gtgggcacct
     4741 ttgctcaccg tctgcctgcg gacatggtgg tgactaacga gaaacaccgt gatatttgcg
     4801 agaagaagtg gaatatcccg agcggcacca ttccggcgaa aatcggtctg cacgcggtgg
     4861 cacaagaccg tgcactgaaa gacggcaagc tgaatgttta ctggaccatg tgtaccaaca
     4921 acatgcaggc cgggccgaac attaatgaag agcgtatgcc gggctggcgc gatccgcgca
     4981 acttcatcat cgtctccgat ccgtatccga cagtcagtgc gctggcagcc gacttgatcc
     5041 tgccgaccgc aatgtgggta gagaaagagg gcgcttacgg taacgccgaa cgccgtactc
     5101 agttctggcg tcagcaggta caggcgccag gcgaagcgaa atcggatctc tggcagttag
     5161 tccagttctc ccgccgcttc aaaactgaag aagtatggcc ggaagagctg ctggcgaaga
     5221 aaccggaact gcgtggcaaa acgctgtacg aagttctgta tgccaccccc gaagtgagca
     5281 aataccctgt atccgaactg gcggaagatc agctgaacga tgaatcccgc gagctgggct
     5341 tctatctgca aaaagggctg ttcgaagagt acgcatggtt tggtcgcggt cacggtcacg
     5401 atctggcacc gttcgatgac taccacaaag cgcgcggtct gcgctggccg gtggtgaacg
     5461 gtaaagaaac gcagtggcgt tacagcgaag gtaacgaccc gtacgtgaaa gcgggcgaag
     5521 gctataagtt ctacggtaaa ccggatggca aagcggtgat cttcgcactg ccgttcgaac
     5581 cggcggcgga agcaccggat gaagagtacg acctgtggct ctctaccgga cgcgttctgg
     5641 agcactggca caccggcagt atgactcgcc gtgtgccgga actgcaccgc gccttcccgg
     5701 aagcggtctt gtttattcac ccgctggatg cgaaagcgcg cgatctgcgc cgtggcgaca
     5761 aagtgaaagt ggtttctcgc cgtggcgaag tgatctcgat tgttgaaacg cgcggtcgta
     5821 accgtccgcc acagggcctg gtgtacatgc cgttcttcga cgccgcacag ctggttaaca
     5881 aactgacgct ggatgcgacc gatccgctct cgaaagagac ggacttcaag aagtgcgcag
     5941 tcaaactgga gaaggtgtaa gccattatgt cccggtcagc gaaacctcaa aatggtcgcc
     6001 gccgctttct gcgcgatgtc gttcgcacag caggcgggct ggctgccgtg ggtgtggcgc
     6061 tggggttaca acagcaaacc gcacgcgcat ctggcgtgcg gttgcgcccg cccggagcca
     6121 taaacgagaa cgcctttgcc agtgcctgtg tgcgttgcgg tcagtgtgtt caggcttgcc
     6181 cttacgacac cttaaaactg gcgacgctgg cctctggtct gtcggcgggc acgccatatt
     6241 ttgtcgcacg ggatattcct tgcgaaatgt gtgaggacat tccgtgcgcc aaagtgtgcc
     6301 caagcggtgc gctggatcgt gagattgaat cgatcgacga cgcgcggatg gggctggcgg
     6361 tactggtgga ccaggaaaac tgtctcaact ttcaggggct gcgctgcgat gtttgttatc
     6421 gcgaatgccc gaaaattgat gaggccatca ccctggagct ggagcgcaac acgcgtaccg
     6481 gtaagcacgc ccgctttctg ccgacggttc acagcgacgc ctgtaccggt tgcggtaagt
     6541 gcgaaaaagt atgcgtgctg gaacaaccgg caatcaaggt gttaccgctg tcactggcga
     6601 aaggggagtt aggtcaccat taccgcttcg gttggctgga ggggaacaat ggcaaatcgt
     6661 aaacgtgacg ccgggcgcga ggcgctggag aaaaaaggct ggtggcgcag tcaccgttgg
     6721 ctggtgttac gtcgcctttg tcagttcttc gtgctgggga tgtttttgag cggtccatgg
     6781 tttggtgtgt ggatcttgca cggcaactac agcagtagcc tgttattcga caccgttccg
     6841 ctgaccgatc cgctgatgac gctgcaaagt cttgccagtg gtcatctgcc tgccacggtg
     6901 gcgttgaccg gtgcggtgat tatcaccgtg ctctacgccc tggcgggaaa gcgattattt
     6961 tgcagctggg tctgcccgct gaacccgatt accgacttag cgaactggtt acgcaggcgc
     7021 tttgacctca atcagtctgc gacgatccct cgccacattc gctacgtgct gttggtggtc
     7081 attctgctgg gatcggcctt aactggcacg ctcatctggg aatggatcaa ccccgtatct
     7141 ctgatggggc gcagcctggt aatgggcttc ggcagcggcg cgctgcttat tctcgcgctg
     7201 tttttatttg atctactggt cgttgaacac ggctggtgcg ggtacatttg tccggttggc
     7261 gcgctgtatg gcgtgctggg tagcaaaggt gtgattaccg ttgcggccac cgatcggcag
     7321 aaatgtaacc gctgtatgga ttgttttcat gtttgcccgg aaccgcatgt gctacgtgcc
     7381 ccggtgctgg atgaacaaag cccggtgcag gtcaccagcc gcgattgcat gacgtgcggt
     7441 cgctgcgtgg atgtctgttc tgaggatgta tttacaataa ctacacgatg gagttcggga
     7501 gcgaaatcat gaaaagccat gacctgaaga aagcgctgtg tcaatggacg gcgatgctgg
     7561 ccctggtggt aagcggcgcg gtttgggcgg ctaatggcgt cgattttagc cagtcgccgg
     7621 aagtatccgg gacacaggaa ggggccattc ggatgccaaa agagcaggaa cggatgccgc
     7681 tgaactatgt gaatcagccg ccaatgatcc cgcatagcgt tgaaggttat caggtaacga
     7741 ccaataccaa ccgctgcttg cagtgccacg gtgtcgaaag ttatcgcacc actggcgcgc
     7801 cgcgtatcag tcctactcac tttatggaca gcgacggcaa agtgggcgcg gaagtggctc
     7861 cgcgtcgtta tttctgtctg caatgtcacg taccgcaggc cgataccgcg ccaatcgtgg
     7921 ggaatacctt taccccatca aaaggttacg ggaaataaga ggttattatg ggaaattctg
     7981 accgtaagcc tggtctgatt aagcgcctgt ggaaatggtg gcgtaccccc agccgtctgg
     8041 cgctggggac gctgctgttg atcggttttg ttggcggcat cgtcttctgg ggtggcttta
     8101 acaccgggat ggaaaaagcc aataccgaag agttctgcat tagctgccac gaaatgcgca
     8161 acacggtgta tcaggaatac atggattccg tgcactacaa caaccgtagc ggcgtccgtg
     8221 cgacctgtcc ggattgtcac gttccgcacg agtttgtgcc gaagatgata cgcaagctca
     8281 aagcaagtaa agagctgtat ggtaaaattt ttggcgttat tgacacgccg cagaaatttg
     8341 aagctcatcg tctgacgatg gcacagaatg agtggcggcg catgaaggac aataactcgc
     8401 aggagtgccg taactgtcac aacttcgagt atatggatac aaccgcccag aaatcggttg
     8461 ccgcgaagat gcatgaccag gcggtgaaag atgggcaaac ctgtattgat tgccataaag
     8521 ggatagcgca caagctgccc gatatgcgtg aagtcgagcc aggtttttaa cagggttatt
     8581 gcgtgggtat gcttgaagcc agagagttac tttgtgagcg ggatgaacga accttattta
     8641 gtggcttgtc atttacgctg aacgcaggag agtgggtaca aatcaccggt agcaacggcg
     8701 cggggaagac aacgcttctc cgtttgctga cggggttgtc tcgccctgac gcaggcgagg
     8761 ttctctggca agggcagccc ttgcatcagg tacgcgacag ctaccatcaa aacctgttat
     8821 ggataggcca tcagccgggg atcaaaaccc ggctgacggc gttagaaaat ctgcactttt
     8881 atcatcgcga tggcgatacc gcacaatgtc tggaagccct ggcgcaggcc gggcttgccg
     8941 gattcgaaga tattcctgta aatcagctct cggccgggca acaacgccgc gtcgctttag
     9001 cgcgtctgtg gctgacccgt gccacgttat ggatcctcga cgagcctttt accgcgattg
     9061 acgttaacgg cgtcgatcgt ctgacccagc gtatggcgca gcatacggag caggggggga
     9121 ttgtgattct gactacccac cagccgctca acgttgctga aagtaaaatt cgccgcattt
     9181 cactgacgca aacgagggcc gcatgatgtt ctggcgcatt ttccgtcttg agctgcgtgt
     9241 agcgtttcgc catagcgccg aaatcgccaa cccgctgtgg ttcttcctga ttgtaattac
     9301 cctttttccg ctcagtatcg gtccggagcc gcaactgctg gcgcgtattg caccgggcat
     9361 tatctgggtt gctgcactgc tttcatcctt gctggcgctg gaacgactgt tccgtgacga
     9421 tttgcaggac ggcagtcttg aacaattgat gttgttgccg ttacccttgc ccgccgttgt
     9481 gctggcgaag gtgatggcgc actggatggt aagcggtctg ccgttactca ttctttcgcc
     9541 actggtagca atgctactgg gaatggatgt ttatggctgg caagtgatgg cgctgacgct
     9601 gctgctggga acgcctacgc ttggctttct cggtgcaccg ggcgtggcgc tgacagtggg
     9661 acttaagcgc ggtggtgtgc tgctcagcat actggtgtta ccgctgacta tcccattact
     9721 catcttcgcc accgccgcga tggacgcggc ttccatgcat ttgcccgttg acgggtatct
     9781 ggcaatttta ggcgcgttgc tggcaggcac cgcgacatta agtccttttg cgacggcggc
     9841 agcgttacga atcagcattc aataacgctg gattactgat ttttgttcga gtctggtatc
     9901 gaaactatgt ggaaaacact gcatcaactg gcgatcccac cacggctgta tcaaatctgt
     9961 ggctggttta taccgtggct ggcaattgcc agtgtggtcg tgcttaccgt cggctggatc
    10021 tggggattcg gctttgctcc ggctgattat cagcagggaa atagctaccg cattatctac
    10081 ctgcatgtgc ctgcggcgat ctggtcgatg ggcatttatg catcaatggc agtggcagcg
    10141 tttattggcc ttgtctggca gatgaaaatg gccaacctgg cggtggcggc gatggccccc
    10201 attggtgccg tgtttacctt tattgccctg gttaccggct ctgcatgggg aaaaccgatg
    10261 tggggcacct ggtgggtatg ggatgcacgt ctgacttctg aactggtgct gctttttttg
    10321 tatgtgggtg tgattgccct gtggcacgcc ttcgacgacc gccgtctggc gggccgtgcg
    10381 gcaggtatcc tggtgctgat tggcgtggtg aatctgccga ttattcatta ctccgtggag
    10441 tggtggaaca ccctgcatca gggatcaacg cggatgcagc aaagtatcga tccggcgatg
    10501 cgttcgccgc tgcgctggtc gatttttggc ttcctgctcc tgtctgccac gctgacgctg
    10561 atgcggatgc gtaatttgat tttgctgatg gaaaaacgcc gtccgtgggt gagtgaactg
    10621 atactgaaaa gaggccgtaa atgacccctg catttgcttc ctggaatgaa tttttcgcaa
    10681 tgggcggtta cgcctttttt gtctggctgg cggtggtgat gaccgttatt ccgctggtgg
    10741 ttttggtcgt gcactcggtg atgcagcatc gcgcaattct gcgtggtgtg gcgcaacagc
    10801 gggcgcgtga ggcgcgttta cgtgctgcgc aacagcagga ggctgcatga atattcgccg
    10861 taaaaaccgc ttgtggattg cctgtgctgt tctggcaggg ttggcactga ctatcggtct
    10921 ggtgctatat gcgctgcgct cgaatatcga tctcttttat acgccggggg aaattctcta
    10981 cggcaagcgt gaaactcagc aaatgccgga agtcggtcag cgtctgcgcg ttggcgggat
    11041 ggtgatgccg ggtagcgtgc agcgcgatcc caattcgcta aaagtaactt tcaccattta
    11101 cgacgctgaa ggttcagtag atgtctctta cgaaggcatt ttgccggatc tgttccgtga
    11161 agggcagggc gttgtggtgc agggcgaact ggaaaaaggc aatcatatcc tcgcaaaaga
    11221 agtgctggcg aaacacgacg aaaactacac gccgccagaa gttgagaaag cgatggaagc
    11281 caatcaccgt cgcccggcga gtgtttataa ggacccagca tcatgatgcc ggaaattggt
    11341 aacgggctgc tgtgtctggc gctaggaatt gcgctgctgc tgtccgtgta tccgctatgg
    11401 ggcgtagcgc gcggagatgc gcgcatgatg gcgtcttccc gcttgtttgc ctggctgctg
    11461 tttatgtctg tggctggcgc atttctggta ctggtcaatg ccttcgtggt caacgacttc
    11521 accgtcacct atgttgccag caactccaat acccagcttc cggtgtggta tcgcgtggcg
    11581 gctacctggg gcgcgcatga aggctcgctc ctgctgtggg tgctgctgat gagcggctgg
    11641 acctttgcgg tagcgatttt tagtcagcgt attccgctgg atattgtggc ccgcgtactg
    11701 gcgataatgg ggatggtcag cgtcggcttt ttgctgttca ttctctttac ctctaacccg
    11761 ttctcacgca cgttgccgaa cttcccgatt gaagggcgcg atcttaaccc gctgttacag
    11821 gatccggggc tgatcttcca tccgcctctg ctctatatgg ggtacgtggg gttctcggtg
    11881 gcgtttgctt ttgccattgc ttctttgttg tgcgggcgtc tggacagcac ttatgcgcgt
    11941 tttactcgtc cgtggacgct ggcggcgtgg atcttcttga cgctcggcat tgtgctcggt
    12001 tccgcatggg cctattacga actcggctgg ggcggctggt ggttctggga tccggtagaa
    12061 aacgcctcgt ttatgccgtg gctggtgggg actgcgctga tgcactcact ggcggtcact
    12121 gaacaacgcg ccagcttcaa agcgtggaca ttactgctgg caatcagtgc cttctcgttg
    12181 tgtctgctgg ggactttcct ggtgcgttcc ggcgtgctgg tatcggtaca cgcgtttgcc
    12241 tctgatccgg cacgcggtat gtttatcctc gcctttatgg tactggtgat tggtggttcg
    12301 ctgctgctgt ttgccgcgcg tggacacaaa gttcgctcac gcgtaaacaa tgcgctgtgg
    12361 tcgcgggaat ctctgctgtt agcgaacaac gttttgctgg tcgccgcgat gctggtggta
    12421 ttactgggga cgctgctgcc gctggtgcac aagcaactgg gactgggcag tatttcgatt
    12481 ggcgaaccgt tcttcaacac catgtttacc tggctgatgg tgccgtttgc gctgctgctt
    12541 ggtgtcggtc ctctggtgcg ctgggggcgc gatcgcccac gtaaaatccg caatttattg
    12601 attatcgcct tcatctcaac gctggtgctg tcgctgctgc tgccgtggct gttcgaaagc
    12661 aaagttgtgg cgatgacggt gctcggcctg gcaatggcct gctggattgc tgtgctggca
    12721 attgcggaag ctgcgctgcg tatttcacgt ggcacgaaaa ccaccttcag ttattgggga
    12781 atggtggcgg ctcacctggg gctggcagtg acaattgttg gtattgcctt tagccagaac
    12841 tatagcgttg agcgtgatgt gcgcatgaag tccggcgata gcgtcgatat tcatgaatat
    12901 cgcttcacct tccgtgatgt caaagaggtg actggcccga actggcgtgg cggtgtggcg
    12961 actatcggcg taacgcgcga tggcaagccg gaaacggtgc tgtatgcgga aaaacgttat
    13021 tacaacactg ccgggtcgat gatgaccgaa gcggcaattg acggcggcat cacgcgtgac
    13081 ctgtacgccg cgctcggtga agagctggaa aacggcgcgt gggccgtgcg tctttactac
    13141 aaaccatttg ttcgctggat ttgggcgggc gggctgatga tggcgttggg cggactactg
    13201 tgtctgtttg atcctcgcta tcgtaagcgc gtgagtccgc aaaaaactgc gccggaggcc
    13261 gtatgaagcg caaagtattg ttaattccgt tgattatctt cctggcgatt gccgcggcgc
    13321 tgctgtggca gctggcgcgt aatgccgaag gggatgatcc gaccaatctg gaatcggcgc
    13381 tcattggcaa gcctgtgccg aagtttcgtc tcgaatcact ggacaatccg gggcagtttt
    13441 atcaggcgga tgtgctgact cagggcaaac cagtactgct taacgtctgg gcgacctggt
    13501 gtccgacctg ccgtgcggaa catcaatatc tgaatcagct ttcagcgcag ggcattcgcg
    13561 tggtcggcat gaactataaa gacgatcgcc agaaggcaat cagctggctg aaagagctgg
    13621 gcaatcctta cgcgctaagc ctgtttgatg gcgacggcat gttagggctg gatctcggtg
    13681 tctatggcgc gccagaaacg ttccttattg acggcaacgg catcattcgc tatcgccatg
    13741 cgggcgatct caatcctcgc gtctgggaag aagagatcaa gccgctgtgg gagaaataca
    13801 gtaaggaggc cgcacaatga ggtttttatt gggcgtgctg atgctgatga tctccggctc
    13861 agcgctggcg accatcgacg tgttgcagtt taaagatgaa gcacaggaac aacagttccg
    13921 tcagctcact gaagaactgc gctgcccgaa atgccagaat aacagcattg ccgattccaa
    13981 ctcgatgatt gccaccgacc tgcgccagaa agtgtatgaa ctgatgcagg aaggtaaaag
    14041 taagaaagag attgtcgatt atatggtggc gcgttacggc aacttcgtca cttacgatcc
    14101 gccgttaacg ccgctgaccg tgctgctgtg ggtgctgcca gtagtggcta ttggcattgg
    14161 cggttgggtc atttacgccc gctcgcggcg tcgggtacgc gtggtgccgg aagcgtttcc
    14221 tgaacaaagc gtgccggaag gtaagcgtgc cggatatgtc gcttatttgc cgggcattgt
    14281 ggtggcgtta attgtggctg gcgtcagcta ctaccagacc ggaaattatc aacaggtgaa
    14341 aatctggcag caggccacgg cacaggctcc ggcgttgctg gacagggcgc tggatccgaa
    14401 agccgatccg ctcaacgaag aagagatgtc gcgtcttgcg ctggggatgc gtactcaact
    14461 gcaaaaaaat ccgggagata tagaaggctg gattatgttg ggccgcgttg gcatggcgct
    14521 gggtaacgct agtatcgcca ccgatgcata cgctactgcg tatcgcctcg atccgaaaaa
    14581 cagcgatgcc gcactgggtt atgctgaagc gttgacacgt tcatctgatc ccaacgacaa
    14641 ccgcctcggc ggcgaactgc tgcgccagtt ggtgagaagc gaccacagca atatccgtgt
    14701 gttaagcatg tatgcgttta atgcctttga gcagcagcga tttggcgaag ccgttgccgc
    14761 gtgggagatg atgttgaaac tcttacctgc caacgatact cgccgtgcgg tgattgaacg
    14821 tagtatcgcg caggcgatgc aacatttgtc gccgcaggag aataaataaa aagaggcccg
    14881 atggtgatgc catcgggcta tttttattgt gccccgcgtt gttgcaggaa cagaatggtg
    14941 gctgccacgc gtgagcggac attgagtttg cgcagcagat tgcgaatatg tacttttact
    15001 gtctgctctg aaatattcaa caccgaggca atctgtttat ttgacagccc ctgtgccagc
    15061 tcgtgcagaa catccagctc gcgctccgtc agcacgctga agggatcttc ttccgcgcca
    15121 aacatttcac gttcacgtaa gtactgattg acgcgttcgc taaagacttt gctgcctttc
    15181 gctccagcac gaattgcttc cagcaatact tccgggtcgc tgtctttcaa cagataaccg
    15241 tctgcgcctg cgtctatcag cgcaaagaca tcgctggagg catctgatac ggtcaggata
    15301 ataatttgcg cggtaacgcc atccctgcgc aaggcattga gggtatccag gccactcata
    15361 cctttcatat tgagatccag caagatcacg tcgatatcca gtctattcgc cagatcgatc
    15421 gcgctcgcgc cgtcgcccgc ttcggcgacc acttcaaagc caggatcaag ctccagtaac
    15481 tgacgaacac cgcgtcgcat aagtggatga tcatccacaa tcatcacctg aaaaggcgtt
    15541 gcttcaggca tagtagtctc ctgaggtttt attagaatca ttatgtttga gcgtagcgtt
    15601 ggtaacgcta ccaggcgcgt gaatgttacc ccattttatg tatggacatg tagtgaaaac
    15661 tacaaccttg tcgttcgctt tggcatcatt ttcattataa agacggaatt ttgcatcagc
    15721 ttctcatcaa gaaaaacaac taaaaaatca atcacaatac ttgtgcattt ctttaaacag
    15781 tttttaaaaa ctgactcccg gtatggagct atgggtattt tctgtaccca atgcttttaa
    15841 cagcaattaa tttcatagga tgaaagctca atgcatcaat ctggttctgt ttctctttgt
    15901 cgttccgcaa tatctgttct ggtggctaca gcgttatatt cacccatagc attggcatca
    15961 acagttgagt atggtgagac agttgatggt gttgtcctgg aaaaagatat ccagctggtt
    16021 tatgggaccg ccaataatac gaaaatcaat cctggcggag aacagcatat aaaagaattt
    16081 ggtgtaagta ataatactga aattaacgga gggtatcagt acattgaaat gaatggcgcc
    16141 gcagaatact cagtattaaa tgacggttat caaattgttc aaatgggtgg cgcggcaaac
    16201 cagactacgc tcaataatgg tgtgctacag gtttatggcg cagcgaatga taccacgatt
    16261 aaaggcgggc gcttaatcgt tgaaaaagat gggggggccg tctttgtcgc tatcgaaaag
    16321 ggaggactac tggaggttaa agagggggga ttagcgattg cggtagatca gaaagcaggc
    16381 ggtgctatta aaacaaccac gcgggccatg gaggtattcg gaacaaaccg tctcggtcag
    16441 ttcgatatca agaatggtat tgctaataat atgttgttgg aaaacggcgg aagtttgcga
    16501 gttgaagaaa atgacttcgc ttataatacc actgtagata gtggcggctt actggaggtt
    16561 atggatggcg ggactgtaac tggcgttgat aaaaaagcag gcggaaaatt aattgtctca
    16621 acgaatgcgc tggaagtgag tggtccaaac agtaaaggcc aatttagtat aaaagatggt
    16681 gtgtcaaaaa attatgaact ggatgatggt tccgggctca ttgttatgga ggacacgcag
    16741 gccattgata ctatccttga tgagcatgcc actatgcaat cgctgggaaa ggatactggt
    16801 acgaaagtgc aggcaaatgc ggtatatgat ctcggtcgat catatcagaa tggaagtatc
    16861 acgtattcct caaaagccat ctctgaaaat atggttatca acaatggccg cgctaacgtc
    16921 tgggctggca caatggttaa cgtttcagtc agagggaatg atggcattct tgaggtcatg
    16981 aagccgcaaa taaattatgc acccgcaatg ttggtgggta aggtagtggt ttctgagggc
    17041 gcttctttta gaacgcatag tgccgtggat accagcaaag cggacgtttc gctcgaaaat
    17101 agcgtatgga ccatcattgc cgatatcact acgacgaacc aaaacaccct cctcaactta
    17161 gccaaccttg cgatgtctga cgcaaatgtg attatgatgg atgagccagt gactcgttca
    17221 tcagtgacgg caagtgcgga aaatttcatt acgttgacca ccaataccct gtcgggaaac
    17281 ggcaattttt atatgcgtac cgatatggct aatcatcaga gcgatcagct caacgtcacc
    17341 ggtcaggcaa caggtgattt caaaatattc gtgacggaca ccggtgccag cccggcagca
    17401 ggagatagcc ttacactggt aacaacgggc ggcggtgatg ctgcatttac gttgggcaat
    17461 gccggaggcg ttgttgatat cggtacgtat gaatatacct tgctggataa tggcaaccat
    17521 agctggagtt tggcagagaa tcgcgcgcaa attacccctt caaccactga tgtgctgaat
    17581 atggcggcag cacaaccgct ggtatttgat gcagaactgg acaccgtgcg tgagcgtctt
    17641 ggtagcgtaa aaggcgttaa ttacgatacg gcgatgtgga gttcggcaat taacacccgc
    17701 aacaacgtga ccactgatgc gggcgctggc tttgagcaaa cattgaccgg gctgacgctc
    17761 ggtatcgata gccgtttctc ccgtgaagaa agcagcacaa ttcgcggctt gttctttggt
    17821 tactctcatt ctgatattgg ttttgatcgc ggcggcaaag gcaatgtcga tagctatacc
    17881 ctgggggctt atgccggttg ggagcatcag aacggtgcct atgttgatgg agtggtgaaa
    17941 gttgaccgtt ttgccaacac catccatggc aagatgagta atggggcaac agcgtttggc
    18001 gattacaata gtaacggcgc gggtgctcat gtcgagagcg ggttccgttg ggttgacgga
    18061 ttgtggagtg ttagacccta tctggccttt accggcttta ccacagatgg tcaggactac
    18121 acgttatcaa acggcatgcg cgcggatgtg ggaaataccc ggatattacg cgctgaagcg
    18181 ggaacggcgg taagctatca catggacctg caaaacggta cgacgctgga accctgactg
    18241 aaagccgccg tgcgtcagga atacgccgat tctaaccagg tgaaagttaa tgacgatggc
    18301 aaatttaata atgatgtggc tggaacccgt ggcgtttatc aggctgggat aaggtcatcg
    18361 tttaccccga cgttaagcgg tcatttgtca gtcagctatg gcaatggcgc aggggtagaa
    18421 tcgccgtgga atactcaggc gggtgtggtc tggacgttct gataacaaaa aataaacagg
    18481 ctgtgatgtg tcacggtctg tttatcgaat taattgcaga tataaaaaaa accaaccgta
    18541 aaggttggtt ttttcttggg atttttggca cgagagtagt ttaattgagt atgtaactac
    18601 atgattttaa atagtatgct tgattcaact tttaaaccgt ataactaaac gtataactaa
    18661 aatcatttgc tgaaaaagtg tacaatttaa atgcgagttt tcctgtggta aaagctccaa
    18721 ccgtaacgat gttcgttatg gttgccttga tggtctgcgt ggtcattatg accattcagg
    18781 atttgaaggg tgacgcttcc gacttcctca aattgaggtt tccgaaaata tcagcgggtt
    18841 agcgattaat ctgaccatta attaaactgc gaaaatttcg taattatccc gatatgggga
    18901 tcaccatatc gggattgatg ccggaaaaat cagcttttcg ccagaacggt gacggcctta
    18961 tcgagttttt gccatccgca ataggtgatt gccctgaacg ctgtagcgtc attctggaaa
    19021 aggctaaccg tccctgattc ggtttttatt accgcctcat cagttggggt tataaccgtg
    19081 gcttcaacgt taataatcag cttttgagcg tctatcagca tcatgcgatc cgcctgttcg
    19141 ctcacaatta ccggtattct caaaagctct atctgccaga actcagcatc agtaagctgt
    19201 aaggcgtttt ctacgggcaa gattagcgac gacgtggcaa gcgtgttacc gttcgctatg
    19261 tgctttttga aaagctctat gaagttttcg gcctcatcag caccattaag cacgccctca
    19321 ggggatacgc ccgccacctc ctggtcactg gaaagaaatt ttttatctat ttctttcacc
    19381 gattccgatg tgatcaagtc tctgatcgct gaattggtac caggggtgct aaatttgatt
    19441 agctcgttat tcagcatcac aatagcgcca agtttgcaca gttttagcgc actttcgttt
    19501 acttctccct ctttgacagg gatttcttcg gcttctttaa gccatttact acccttgcct
    19561 gatattgact gcaattttgc gccaaacgga agattcagcg cgctgctgat tagcttgcct
    19621 ggtacagact gcgaaaaaat atactcgcca agctctctta ctatcggctc tgccaccagc
    19681 gacggcatat tatccgtagt cattccggca acaatggatt tttcaagaaa tttcgatact
    19741 gccggatcat tttttttcgt catttcaatt gcgtctgcat ggctgaattt acacgatgta
    19801 agcacgctgg cggcgcgaac tgccgacacg cctttcatgt ttttagaaat cattatcttc
    19861 ttcctcctgg taacatgggg ttagggtttc gtgaaaatat ggcatcagaa cagcggcagc
    19921 ctgtaaccgt atttcggttg gctgctgctt gtcgttcatg atttccgtga gtacatcaat
    19981 agggtctgtg atgtattcgc tcatagttcg ctacctcagt gttttaagcc agttggtggc
    20041 ttagggatgg ggaaccgatt tgaggagaca ttagcggcat tgagtttttt aacctctttt
    20101 ttcccaatgc tgccgatctt gcggtgggtg aactgggcca gcttaaacgc ggcatctaaa
    20161 gccagttttg gatctgtgtc catgttgtca atcagtatcc tgcccattgc tttgatgggg
    20221 tcgggtaggc cgttatccat catctcaatg gtgttaattg atgcccgttc cggtgtaact
    20281 ttttcggtaa ctttctttgt aacttttttc ggttttgtaa cctgaaaagt tacatcagaa
    20341 gttacagcct taagatactt tttcacctcc ggatcattgg atagctgcca gcctttaacc
    20401 cttgcagtct tttcgctgta tcccgcatgt atcgcggctt ccgttggatt tgtgcccttt
    20461 gataacgcat cagcaaaccg cttttttttg gtcgttaatg ccatgcaaaa acctcgtttt
    20521 gagggggtgt aacttttcgt gtaacttttt tgaaaagcaa aattttctat gaatgagact
    20581 gggggcggtg tccagggcga tcgactttcc ggccggaaca ctccccccct gtcattgctc
    20641 accgagtaac gagatcaggc cttcaacttc atcactcagc ttgtttattc gtgtctcaag
    20701 ctctgcgatc ttttccatca atggcgctat ggtgtctctt gtttcctcgc caatggcttt
    20761 aatgatgctt gcctcgcgtt ctgtgatagc cattgtttac gcctcacttg ttgcaatgcc
    20821 caggcgttcg cgtaacagta cgcgcgcaag atgccctatt gttggtgttg ccgccctgcc
    20881 gtagatacct gattcattca tccggcgcac aatatcgaac tgcaactttt taagctcgtt
    20941 aaggatttca tgatcaatca tcacggtagc gctttgcttt atctcttcca ctttgttttc
    21001 tcctgatact tatcattgca taaccagtat tttcgataga ttatcattaa ttaaccatct
    21061 ttgaagatat atgagatcga aaaaaagccg gattgctccg gctatgtgct cacttcttcg
    21121 gttattctcc agcg
//
LOCUS       DAZGLJ010000064        20623 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000064 DAZGLJ010000000
VERSION     DAZGLJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.064"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1251)
                     /locus_tag="ACHK49_002116"
     CDS             complement(<1..1251)
                     /locus_tag="ACHK49_002116"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO9262762.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFV"
     gene            complement(1263..1550)
                     /gene="eutN"
                     /locus_tag="ACHK49_002117"
     CDS             complement(1263..1550)
                     /gene="eutN"
                     /locus_tag="ACHK49_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO9262763.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(1657..1950)
                     /gene="eutM"
                     /locus_tag="ACHK49_002118"
     CDS             complement(1657..1950)
                     /gene="eutM"
                     /locus_tag="ACHK49_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO9262764.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(1989..3005)
                     /gene="pta"
                     /locus_tag="ACHK49_002119"
     CDS             complement(1989..3005)
                     /gene="pta"
                     /locus_tag="ACHK49_002119"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO9262765.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(3002..3805)
                     /gene="eutT"
                     /locus_tag="ACHK49_002120"
     CDS             complement(3002..3805)
                     /gene="eutT"
                     /locus_tag="ACHK49_002120"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO9262766.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(3802..4503)
                     /gene="eutQ"
                     /locus_tag="ACHK49_002121"
     CDS             complement(3802..4503)
                     /gene="eutQ"
                     /locus_tag="ACHK49_002121"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO9262767.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(4478..4957)
                     /gene="eutP"
                     /locus_tag="ACHK49_002122"
     CDS             complement(4478..4957)
                     /gene="eutP"
                     /locus_tag="ACHK49_002122"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO9262768.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(4970..5305)
                     /gene="eutS"
                     /locus_tag="ACHK49_002123"
     CDS             complement(4970..5305)
                     /gene="eutS"
                     /locus_tag="ACHK49_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO9262769.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(5598..7877)
                     /gene="maeB"
                     /locus_tag="ACHK49_002124"
     CDS             complement(5598..7877)
                     /gene="maeB"
                     /locus_tag="ACHK49_002124"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO9262770.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            8166..9116
                     /gene="tal"
                     /locus_tag="ACHK49_002125"
     CDS             8166..9116
                     /gene="tal"
                     /locus_tag="ACHK49_002125"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO9262771.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            9136..11139
                     /gene="tkt"
                     /locus_tag="ACHK49_002126"
     CDS             9136..11139
                     /gene="tkt"
                     /locus_tag="ACHK49_002126"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO9262772.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSMKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGVIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(11235..12278)
                     /gene="ypfG"
                     /locus_tag="ACHK49_002127"
     CDS             complement(11235..12278)
                     /gene="ypfG"
                     /locus_tag="ACHK49_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO9262773.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQVIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(12404..12979)
                     /gene="nudK"
                     /locus_tag="ACHK49_002128"
     CDS             complement(12404..12979)
                     /gene="nudK"
                     /locus_tag="ACHK49_002128"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO9262774.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(13047..15026)
                     /gene="aegA"
                     /locus_tag="ACHK49_002129"
     CDS             complement(13047..15026)
                     /gene="aegA"
                     /locus_tag="ACHK49_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO9262775.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKPRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMNAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            15232..16932
                     /gene="narQ"
                     /locus_tag="ACHK49_002130"
     CDS             15232..16932
                     /gene="narQ"
                     /locus_tag="ACHK49_002130"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO9262776.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            17096..20209
                     /gene="acrD"
                     /locus_tag="ACHK49_002131"
     CDS             17096..20209
                     /gene="acrD"
                     /locus_tag="ACHK49_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO9262777.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(20308..20367)
                     /gene="ypfM"
                     /locus_tag="ACHK49_002132"
     CDS             complement(20308..20367)
                     /gene="ypfM"
                     /locus_tag="ACHK49_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO9262778.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         4725 a         5610 c         5370 g         4918 t
ORIGIN      
        1 aacgaaaatg ctggtatcaa tggcgttcgc catctggttc atgttttcga tattgcgcga
       61 gtgcattgcc gccgtgtggt ggcaaccgcc ctccagtttc accgccagcg caatggcatc
      121 cgccacgttg gcgacgcgca cgacgggcag caccggcatc atcagttcgg tcacggcaaa
      181 cggatgttct gcagtggttt ccacaaacag caaacgcgtt tcttgcggca cgttcaggcc
      241 gattgccgcc gcgattttcg ccgcatcgcg accaacccag tcacggctga cggtgccttt
      301 tccgcgctcg tcgatatttt tcagcaacac cggttgcagc tgctgcgcct gttctgcggt
      361 cagtttcacc gcgtgctggc cttccatcag acgcatcagt tcatcggcta cgctatcaac
      421 aacaatcagt accttttcgt cggcacaaat gatgttgtta tcgaaagaag cgcctttgac
      481 gatggactga gcggcacggg cgaggtcggc ggtttcatcc accactaccg gcgggttgcc
      541 agcgcctgcg gcaatcagac gtttattggt gtgtttacgc gccgcttcta ctaccgcttc
      601 gccgccggtt accaccagca ggccgatacc cggaaacttg aacaagcgtt gcgcggtttc
      661 gatatccgga tttgccacag taaccagtaa gttttccggc ccacctgcgg caacaatcgc
      721 ctggttgagc agcgtaatcg cccgctggga gacttttttc gccgccggat gcggggcaaa
      781 aatgacgctg ttgcccgcgg caatcaggct gatggcgttg ttaattacgg ttgccgccgg
      841 gttagtggaa ggcgtcaccg aagccaccac gccccagggt gcgttttcaa ttagggtcag
      901 gccgttgtcg ccagtcagca cttgcggaga gaggcactca acgcctggtg tgccgcgcgc
      961 ctgagcgacg ttttttgcaa atttatcttc aacgcgcccc atgccggttt cactgacggc
     1021 aagttccgct aaatctctgg cgtgtttttc gcctgcttca cgaatggcag caatggctaa
     1081 ctggcgcatt gccacgcttt ttaacccttg ctgggcgact ttggctgccg caacggcgtc
     1141 atccagggac gcgaaaacgc ccatctcatg aacggcggcg gacggcgtgt cactgctttg
     1201 cattttcagc agtaccgctt tcaccacctg ttcaatatcc tgttgattca tgatgttctg
     1261 ccttatttgt ggaaaattac ctgaccgcca gacaccacct catcgacaat gccaatcacg
     1321 cacagatcga ccggtgacgt ttcgctttta tgcgcctggc gggcggaact gccactcacc
     1381 agtaacaccc actccccggt tcccgcgcca atattgtcga tggcgacggc gcattgcccg
     1441 tcaggattac cttgtggatc aatcatttcc accatcagca atttgtcatg cgccagtccg
     1501 tgatggcgta cggtacaaac aatttgtcca gtgacgactg ccagtttcat acccgcctcc
     1561 gtggcgtatt tcaggtaaaa gctcccccta ccctccgcag aaggtaaaat gaaaaaggag
     1621 agagcgtgac gcccgaatcg acgtcacaca gggtgattac aggttgctgc tatcgccttt
     1681 caggccgatc gggaagactt cttccaggtc gccgtgcgga cgcgggataa cgtgtacaga
     1741 taccagctcg ccgatacgct gtgccgctgc ggcacctgca tcggtcgcgg ctttacaggc
     1801 tgcaacatca ccacgaacca tggcagtaca caggccgccg ccaatctgct taacaccaac
     1861 cagcttgacg cgcgcagctt tcaccatcgc gtcagaagcc tcaatcagtg caaccaggcc
     1921 ccgggtttcg atcattccta atgcttccat tgtgctttcc tctttatcag ggtccagaac
     1981 gggaccgttc attcaaccag tgtttgtaaa ctgctttcgc ggttcacttc tgtctgacgc
     2041 ggcacagctg ccaccagcgc cagctcgata atttcctgca cgctacaacc acgagagaga
     2101 tcgtgcatcg gcgcggcaag tccttgtatc agtggcccga cggcacgata tccgccgagt
     2161 cgttgtgcga ttttgtaacc aatatttccg gcttccagcg acggaaaaac catcacattg
     2221 gccttgccct gtagcgggct ggcaggcgct ttttgcgccg ccacttccgg cacgaaggcg
     2281 gcgtcaaact gtaactcgcc atccaccacc agctttggtg cgcgctcacg gacgatttct
     2341 gtcgcctgct ggacgttagc aacacagggg tgacgggcgc taccattgct ggaaaacgac
     2401 agcatcgcca cgcgcggctc ttctccggtg atggcgcgcc aggtttcggc actggcaagc
     2461 gcgatatccg ccagctgcgc cgccgtcggc tgtggcacca cgctgcaatc ggcaaagccc
     2521 aacgccgggc cgctgtactg tggcaacatc aggaaaatgg aagagagcgt tttacagcct
     2581 ggctgcaagc cgataatgcg taatccggca cgcagcacat ttgccgtgga agagaggttg
     2641 cccgcgatac agacatccgc tttaccggcg ctgaccattg ctgcggcgaa catcagaggg
     2701 tcggtaagtt tttccagcgc atccggcggc gttttttcgc ccgcgcgggc cagccagcga
     2761 tgagcaaatt cttcccgcat tgcgaggttg ccatgcggat caatcacctg tagcccgtcc
     2821 atcgccacgc cgtgactgag cgcaaactga cgaagttcaa acggattggc gaccagaatg
     2881 ggcgttgcca gaccttgttg atgtaaatat tgcgcggctt tcagcacacg ttggtctaac
     2941 gcatccggaa aaaccactct ggcgggcgct cgcaacgcca gttcacgaca acgttcaata
     3001 atcattgcgt ttcccccagt cgttgttgga tctgcttcac cgttagcggc tgcttcgtta
     3061 cgctcaggat catcatcacg tagaccgtgc tggaaaggcg gttcagcgcc tgcaaaatgt
     3121 ccgggcgtaa tacttcaaag ctgcgggtga taaacacctg cgccgctacg gtttcggttt
     3181 cccgcacttt ggtacgtagc agattgagca acgccgcatc gcgcccgtgg ctggcttccg
     3241 gcaccagatg atcgtggtcg agatagcgca gcggctggtg agaaagtcga tgcagatctt
     3301 cgtcagaaag cccgacaatc gcctggcagc ccagcggttc acccagcgca tcggcgcgca
     3361 taatgttgcc aagacgcgaa cggatatccg ccagccacgg ctgccacggt tccgccagtt
     3421 caatttgcag ccacactgcc agcgcaatgg tgctgtcgag aaccgcgcga aaacccagac
     3481 gcggatcgct tttggcgacc attttttccg ccgacaggtg agtcagcgta tccggctttt
     3541 ttgccaccgg ctggcggcac agttcgcagc acgcctgcgg atgttcatcg ctactggtca
     3601 gcccatgaac gggctgcggc tgctgctgtt catcgtcaac aaacaggcgg ccctgctcgt
     3661 caataaactt gatgcgcaga tggcggcttt ccagtaactc ccgggcagag ggcgtcaggc
     3721 gactgtccgc agggagatgg atctctgcgc cttcgctgag cgtatggttc gctcttagcc
     3781 atgcttcggt gatgaaatct ttcataggga ttgccagtta gccggccagg cgacatacag
     3841 gaatttcacg ctggatgtcg taccaaattc gatactggag cctttcggga taaacatcac
     3901 gtcacccgct ttggcgatca tggtttcgcc ttcgtggcga acatgcagct ccccttccag
     3961 caccatgtcg atttcgtcgt agttcagggt ccacgggaag aatgcgttct cccactgcat
     4021 aaaaccagcg gccatgctgc tgccgtcgtc tccggtcaca agatcagtca agcccacgca
     4081 atgcggctct gcgccgtcga aacgaccaaa cttgacgctg ctgccatcaa tcactttgat
     4141 gccgcctttg ccggtcaccg atttgaagct cggctgcatt gcgccctgct ccagcgactg
     4201 tttttccttc atcacttttt ccatcagctg tgcgaccagg ctttcggtaa actggccttc
     4261 cggcagctgg gcaatgatgg tttcgcggat gcgctggctt tcggttttgt cggctggcac
     4321 gctggcgggc acggacgccg ttaccgggat cgattcatca cattcggtga tggtaaaacc
     4381 cagtagatcc gccacttcac gggcttccgg ggtaataatg ctggcgcgca gaaccacgga
     4441 cattgcctgt tcgccgcgcg cgtgtgcttc acgaatatca ttcgctgtga tgagtttttt
     4501 cacctgcttc ctcctgtttg gtgagagatg ccagataatc caccagttgc tgcacgcttt
     4561 gcgggtcgtg gctgtttagc tcgaaaatcg gctcttcgaa acccgtctca agcaataact
     4621 ttcgcgttgc ggcgacgtca gcatctggca tgtccgtttt actgatgacg gcgatttgcc
     4681 gtttgctgac gccaatatcc agcaacccgg caggcaaccg actttccggg tcatttgcgc
     4741 catggacata gatcagcatg tctacatcct gtagcgtggt aattaaggcg tgataccagc
     4801 ggggatggct aaaatattca cccggcgtat caatatcgcc cttatcatta aattccacgg
     4861 cctgtgtttt tctggcgagg gtataattcc cctgcaacgc attaaataac gttgtttttc
     4921 ctgccccgac ggtacccaca aacgcaattc gtttcatgaa tgcctcgtat taacttttgg
     4981 tcatttcaca aagcgtataa tttaataagc gacctaaacc gctgacggtt tgtgataacg
     5041 cttcttctac tgcgcccacc gagccataaa tcaccaatgc accgctgaag cggtcgagaa
     5101 aaccgatatg cacatcagcg gctttcagcg ctaaatcacc ggcgatcatc gccgtttcgc
     5161 cgggagttag cgtcataatg ccaattgcgc ccgcatccgg aacgccgatt ttcttcgcca
     5221 gttcctcacc gggatgcgcg atgagatgcg ccagtgtgac ctgtttgccg ggcacaaatt
     5281 cctgaatgat gcgttcttta tccatgagtc gccgcctgtg aaaaactttc gtgtatcgtg
     5341 acaatttttc gtcagagtaa ataacaaaat tcggcaagcg cgtttaaaag gtgaggtaga
     5401 tcactaagaa tggagagata ttcgctgtgg tgcataaact ttcatatgca acgcatgaat
     5461 ataaataaaa taatatcgag atattatata gactgctttt tttattattc gcaaactcag
     5521 taataagctt tactaatata ttgcagccaa accgcttacc ctgaatattc agggtaagcg
     5581 tgagagttaa aaaagaatta cagcggttgg gtttgcgctt caaccacggc cagcgccacc
     5641 atgttgacga tacgacgcac cgatgcgatc ggcgttaaca cgtgaaccgg tttcgcaacg
     5701 cccatcagca ccggaccgac agtcacacct tccgagctgg aaacgcgcag taagttgtaa
     5761 ctaatgcggg cagcttccat gttcggcatc accagaatat tggcagaacc tttcaaaggg
     5821 ctgtccggca tacggtcgtt gcgaatcgct tccaccagcg ctgcatcgcc gtgcatttca
     5881 ccatcaatca tcagatctgg cgcacgatcc ctgaccagtt ccagcgcctg acgcattttg
     5941 ctcgacgacg ggcagtcaga agaaccaaag ttggagtgcg acaacagagc aacgcgcggc
     6001 tcaataccaa aacgacggac agtttctgcc gccatcaagg tgatctccgc cagctcttct
     6061 gcgtctggtt cgtcattgac gtaggtatcg gcaataaagg tgttaccact cggcagcagc
     6121 agcgcgttca ttgcacctgc ggtgtgaacg ccatcgcgat aaccaaagac atttttcacc
     6181 acgctaaaat gttcatgata atcgcccacc gtaccgcaga tcattgcatc ggcttcccca
     6241 cgctgaacca tgatcgcgcc gatcactgtc gggttactga tcagcgcccg ctgcgcctgt
     6301 tcctgagtga caccgcgacg cttcatgatc tggaagtatt cggtccagta ctctttaaag
     6361 cgcggatcgg attcgttatt gacgatctca aaatcaacgc ccgctttgat ctgcaagccc
     6421 agtttctgaa tgcgcatttc gatcacgttc ggacgaccga taaggatcgg tttcgccagt
     6481 cccagcgtta ccagttcctg agtggcatgc agaacgcgcg cctcttcccc ttctggcaga
     6541 acaacgcgct tcggcgcttt gcgagcctgg gagaaaatcg gcttcataaa caggttggtt
     6601 ttgtaaacga actcagtcag cttgtcgatg tagacgtcga aatcagcaat cggacgcgtc
     6661 gccacgcccg actccatcgc ggctttagcg accgcaggag cgatcttaac gatcaagcgc
     6721 ggatcaaacg gttttggaat gatgtattcc ggaccaaagc tcagatcctg atcgccatac
     6781 gctgaagcca ccacttcgct ctgttcggcg tgggccagtt ccgcaatcgc gcgtaccgcc
     6841 gccagtttca tctcttcatt gatagccgtt gcgccaacat ccagcgcgcc acggaagata
     6901 aacgggaagc aaaggacgtt gttgacctgg tttggatagt cagaacgacc ggtgcaaatg
     6961 atggcatccg gacgcacttc tttcgccagc ggcggcagaa tttccggttc cgggttcgcc
     7021 agcgccagga tcattggcgc acgagccatt ttcttcacca tttcctgggt cagcactttc
     7081 gggccggaac atccaaggaa aatatccgcg ccttcaatca catcatcgag ggtacgtttg
     7141 ccgtcatcca ccaccgcata tgcggctttg gtttccgcca tgtttggctc acgcccctga
     7201 tagataacgc cttttgaatc gcaaaccacg atgttatgtt tttgcagacc cagcgctacc
     7261 agcaggttca tacaggcgat tgctgcggca cccgcgccgg aaaccaccat ccgcacgtcg
     7321 gagatgtttt tctccaccac gcgcaagccg ttgaggatgg cggcagtgct gatgattgcc
     7381 gtgccgtgct gatcgtcgtg gaataccgga atattcatcc gctcgcgcag tttctgttca
     7441 atatagaaac attctggcgc tttaatgtct tcgaggttga ttccgccgaa ggtcggttcg
     7501 agcgcagcga caacttcaat aaatttgtcc gggtcgagtt catcaacttc aatgtcaaat
     7561 acatcaatcc cggcgaattt cttaaacaga acgcccttgc cttccatcac cggtttgcca
     7621 gccagcgcgc caatgttgcc taaccccagc accgccgtac cgttagagat caccgccacc
     7681 aggttaccgc gcgcggtata tttgtaggct tttaacgggt ctttttcgat ttcaagacaa
     7741 ggtgcggcaa cgcctggtga gtaggccagc gccagatcgc gctgcgttgc cagaggcttg
     7801 gttggagaaa cctggatttt ccctggaact ggaaattcat ggaaatcaag tgcactttgt
     7861 tttaactggt catccatttg gttgttcctt tcacgtaacg ttcacaaata aagtgtgtgg
     7921 gcaacagccc ctgcccacaa cgtggcgcac attattaccc tgccggagtc tacagacttt
     7981 gagcaagtcc aaactctcac cattaatata atgttttggt aataatccta taacactgat
     8041 gttacctgct taatccagca ataccatgcc tgtctgctat gcttttttga tgcgtttagc
     8101 gaaatttctc agaagtgtga attaacgcac tcatctaaca ctttactttt caaggagtat
     8161 ttcctatgaa cgagttagac ggcatcaaac agttcaccac tgtcgtggca gacagcggcg
     8221 atattgagtc cattcgccat tatcatcccc aggatgccac caccaatcct tcgctgttac
     8281 tcaaagctgc cggattatca caatatgagc atttaataga cgatgctatc gcctggggta
     8341 aaaaaaatgg caagacccag gaacaacagg tggtcgcagc gtgtgacaaa ctggcagtca
     8401 atttcggtgc tgaaattctc aaaatcgtac ccggtcgcgt gtcaacagaa gttgatgcac
     8461 gcctctcttt tgataaagaa aagagtattg aaaaggcgcg ccatctggtg gacttgtatc
     8521 agcaacaagg cgttgagaaa tcacgcattt tgatcaaact ggcttcgacc tgggaaggaa
     8581 ttcgcgcggc cgaagagctg gaaaaagaag gtattaactg caacctgacg ctgctgtttt
     8641 cttttgccca ggcgcgggcc tgtgcagaag cgggcgtttt tctgatttcg ccgtttgtcg
     8701 ggcgtattta tgactggtat caggcacgca agccgatgga cccgtatgtg gtggaagaag
     8761 atccgggcgt taaatcggtg cgcaatatct acgactacta taagcaacac cactatgaaa
     8821 ccattgtgat gggcgcgagc ttccgtcgca ccgaacaaat cctcgcctta accggctgcg
     8881 atcgactgac tatcgcaccg aatttgctga aggagctgca ggaaaaagtt tcgccagtgg
     8941 tacgtaaatt aatcccgcct tctcagacgt tcccacgccc agcacccatg agcgaagcgg
     9001 agttccgttg ggagcacaat caggatgcga tggcggtaga aaaactgtct gaaggcattc
     9061 gtctgttcgc tgttgatcaa cgcaaactgg aagatcttct tgccgccaaa ctataaacca
     9121 gccacggagt gttatatgtc ccgaaaagac cttgccaatg cgattcgcgc actcagtatg
     9181 gatgcggtac aaaaagccaa ttctggtcat cccggcgcgc cgatgggcat ggctgatatt
     9241 gccgaagtgc tgtggaacga ttttcttaaa cataatccta ccgacccaac ctggtatgat
     9301 cgcgaccgct ttattctttc caacggtcac gcgtcgatgc tgctctacag tttgctacat
     9361 ctgaccggtt acgacctgcc gctggaagaa ctgaagaact tccgtcagtt gcattcgaaa
     9421 accccaggcc acccggagat tggctatacg ccaggcgttg aaaccaccac cggcccgctt
     9481 ggacaaggtt tggcgaacgc cgtcgggctg gcgatagcag agcgtacact ggcggcgcag
     9541 tttaaccagc cagaccatga gatcgtcgat cacttcacct atgtgtttat gggcgacggc
     9601 tgcctgatgg aaggtatttc ccacgaagtc tgttcgctgg caggcacgct gggactgggc
     9661 aagctgattg gtttttacga tcacaacggt atttccatcg acggtgaaac agaaggctgg
     9721 tttaccgacg atacggcaaa acgttttgaa gcctatcact ggcatgtgat ccatgaaatc
     9781 gacggtcacg atccgcaggc ggtgaaggaa gcgatccttg aagcgcaaag catgaaagat
     9841 aagccgtcgc tgattatctg ccgtacggtg attggctttg gttcgccgaa taaagcaggt
     9901 aaggaagagg cgcacggcgc accactgggg gaagaagaag tggcgctggc acggcaaaaa
     9961 ctgggctggc accatccgcc atttgagatc cctaaaaaga tttatcacgc ctgggatgcc
    10021 cgcgaaaaag gcgaaaaagc gcagcagagc tggaatgaga agtttgccgc ctataaaaag
    10081 gctcatccgc aactggcaga agagtttacc cgtcggatga gcggtggttt accgaaggac
    10141 tgggagaaaa cgactcagaa atatatcaat gagttgcagg cgaatccggc gaaaatcgcc
    10201 acccgtaagg cttcgcaaaa tacgcttaac gcttacggac cgatgctacc ggagctgctc
    10261 ggaggttcag cggatctggc tcccagcaac ctcaccatct ggaaaggctc tgtgtcgctg
    10321 aaggaagatc cggcgggcaa ctacattcac tacggggtgc gtgaatttgg catgaccgct
    10381 atcgccaacg gcattgcgca ccacggcggc tttgtgccgt ataccgcgac gttcctgatg
    10441 tttgttgaat acgcccgtaa tgccgcgcgg atggcggcac tgatgaaagc acggcagatt
    10501 atggtttata cccacgattc tattggctta ggggaagatg gtccgacgca ccaggccgtt
    10561 gagcaactgg ccagtctgcg cttaacgccg aatttcagca cctggcgacc gtgcgatcag
    10621 gtggaagcgg cggtgggctg gaagctggcg gttgagcgcc acaacgggcc gacagcactg
    10681 atcctctcaa ggcagaatct ggcccaggtg gaacgtacgc cggatcaggt taaagagatt
    10741 gctcgtggcg gttatgtgct gaaagacagc ggcggtaagc cagatattat tctgattgcc
    10801 accggttcag agatggaaat taccctgcaa gcggcagaga aattagcagg agaaggtcgc
    10861 aatgtacgcg tagtttccct gccctcgacc gatattttcg acgcccagga tgaggaatat
    10921 cgggagtcgg tgttgccttc taacgttgcg gctcgcgtgg cggtggaagc aggtattgcc
    10981 gattactggt acaagtatgt tggtctgaaa ggggtaattg tcgggatgac gggttacggg
    11041 gaatctgctc cggcggataa gctgttcccg ttctttggct ttaccgccga gaatattgtg
    11101 gcaaaagcgc ataaggtgct gggagtgaaa ggtgcctgat ggtgatcgcc ggatgctgat
    11161 tgccggatgc gacgctgacg cgtcttatcc ggcctacagc gtcttatccg gcctacatgt
    11221 ccccgcaatt ttgtttaacg ggtgatccac aacgtgggcc aggcgtctgg cccatgccag
    11281 ttatcgcagg tgggttctgc ggcataacgc accaggcgaa aacgctgacc gtcaaagcgc
    11341 cagcgcgcct gaatgccaca atcgcttaat ccgcgccctt tcgctaaggt caccagttca
    11401 cgcgatttct catcaaatgt tgcgttcatc agttccagtt cattcgtctc ctgaccgttg
    11461 ttgaatggca aacgcaaccg aaccgggcgc gaggctagtg gctttttacg cgacacaatc
    11521 catgccaaat caatggtgtt ataggcccct gcctcacagc taatcatcat cagcgcttta
    11581 tcatcagtca gcgcagtgac attcacctca cgacgtaacg gatcaagcga gcagcgcaga
    11641 ccattcatcc gccagtttcc ataatccagc aaatcgttgc gttcttcgag tgagagtggc
    11701 gtcggcgttg ggttaaccac cgcgacctct ttcagcgcag gcgcgggcgg tacgctgagc
    11761 ggcggttcgt cccctttctt gatccacgcg gtttcactgc caacgcgctt ttgctgagca
    11821 tcaataaaca acaacgccgc ttttaagcca ctcagagaaa tggtctgatt gccgtcgcgt
    11881 aaggtgatcg ccttcccttc ctgaatcacc tgcaaaaacg cggtgatggt tgccgtatca
    11941 tcggttacta ataaccatgg tgaaatccgc cacttgtcac cacttaacgc caacggctca
    12001 ccatctaaca gcagccgtgg cgctatctct ccttcagacg catccggcga cttcaacccg
    12061 ccgcgctcaa tacgtaaaac ggcatcggta tgcgccccgg cgctgcggct cagggtcatc
    12121 accagtccat tatgatcgcc cgtattgcgc gccacgcaga agttttggtt attacaggtg
    12181 acctgccagt cggaaaacgc ccgttgcgct ggtgccgccc acaccaaaga cgtcggcaac
    12241 aaagcgaaaa aaaagagaag gaaaatgcga tagcgcatgg acggtacgac cccagaagtt
    12301 caaacaaaca gcacaagtcg tatcttcgtt gcgcaggtag cgcggctcaa tcagatttgt
    12361 cggattgagc caataataaa caacgaaata ttattgttat ttttcagtcc attaaatgtg
    12421 acgtttgcaa atagttaagc aataacaccg tcttaccgtc acgtatctcg ccggttttga
    12481 tcatctccag cgcctggctg aacggcagct cgagcacttc aatatcttca tcttcaacac
    12541 cgccaccggc gttagcgcgc tgattgtcac tgtattcggc gataaaaaag tggattagct
    12601 cagtcacacc gccaggcgac atatacagtt caaataattt gcgcacttcg cccacttcat
    12661 agcccgtctc ttcaatcgct tctttacgaa tgcacacttc cggttcgtcg ttatccagca
    12721 gcccggcgca ggtttcaatc agctgcccgc tttcattgcc attaacccag gtagcgacac
    12781 ggaactgacg aatcagaacc acgctctttt tcttcgcgtt gtacaggagg atcgtcgcac
    12841 cattaccgcg atcgtaaact tcacgtttat ggcggataac ttcgccatct ttgcgcgtga
    12901 gatcgtaggt aatgttgtgc agggtgaaat agttatcgga gagaattttg tctttaatga
    12961 gggtgatttg ttgcgtcata ccgactccac agcgcgaaat gaacaattat cttacgctgt
    13021 gaagtcgggt ttgtctgcgc aggctatcag tgagatttga ctgattttac ccccagccag
    13081 tcaataatcc cctgtgccgc atgacgtcct tctgccattg cggtaaccac cagatccgca
    13141 ccacgcacgg cgtcgccacc ggcgaagatt ttcggattgg tggtctggta acggtactgg
    13201 ctttccacat ccgcgatgat gcggccccat ttgtctaccg ttacaccgtg cgactccagc
    13261 cacggcatcc cgtgtggatt gaagccaaac gccataatca ccgcatcggc tggcatgaca
    13321 aattcactgc cctcaatcgg tacaggtcgc cgacgcccct gggcatccgg ctcgccaaga
    13381 cgcgtgcgca ggaaacgaat cccgcagacg tgaccttgtt cattcagctc aagcgccacc
    13441 ggctggacgt taaattcgaa gttggccccc tcttcgcggg cgttcctcac ctctttcttc
    13501 gagcctggca tgttagcttc atcacgacga taagcgcagg tgacgttact cgcgccgtgg
    13561 cgcagcgcag tacgcacaca gtccatcgcg gtgtcgccgc cgcccagtac cacgacgtta
    13621 agtccggcgg tattgataaa cggctcttcc ggtagctctt cgagccccat cacctgttta
    13681 gtgttggcaa tgaggaacgg cagcgcgtca taaacgcccg gcgcatcttc attgggtaaa
    13741 cccgcgttca tggaacggta agtgcctacg ccaacgaaga ccgcgtcgta ttgttccaaa
    13801 agcgaatcca aagagacatc tttacccact tcacaattga gttcgaagtg aatccccatc
    13861 gcgctgaaga tttcccgacg gcgtgccagc agggatttat ccagtttgaa agaaggaatg
    13921 ccgaaagtga gcaagccacc gatttctgga tggcgatcgt acaccgtcac cgccacgcca
    13981 ttgcgggtca gaacatccgc acaggccagc cctgccggac ctgccccgat aatcgccacc
    14041 cgcttgtcca ctttggtgac atgacttaag tcaggacgcc aacctttcgc caacgcctga
    14101 tctgaaatat agcgttcaat attgccgata gttaccgcgc cgtgctcatc gcgaatagta
    14161 caggcacctt cacacaaacg gtcttgcgga caaacgcgtc cggtaatttc cggcagggtg
    14221 ttggtctggt gagaaagctc gacggcggcg tcgatgtttc cggctttcac cagttcaatc
    14281 cactgcggaa tatggttatg cagcgggcag gtccattcac aaacgctgtg ctcgccgcac
    14341 ttaaggcagc gcgaggcttc ccgttgtgcc tggtcggcgc gaaatggcag ataaatttca
    14401 tcaaaaccgg ttttgcgcgc ttcaatcgcc agtttatccg gctcgccacg cgcgggcgtt
    14461 gcctgcattt gttcgacttt actcattacc ggcatttctt gcgccgcggt actggcatgc
    14521 cacggttgat gttcctgacg cgcggtgcgc aagcggcggg gtttcgccat gccggagagt
    14581 gcgacgtcag tgaccagttg cagcgcgtcc gccgggcaat tctcaacaca ggcaggaccg
    14641 ttttcgcgcc ccgcacaaag gtcgcattta tgcgccgtgg cttttacttt tcctgccgcg
    14701 acgggtgtca ggacgatttg catcgtacca aaaggacagg ccaccacgca ggatttacag
    14761 ccaatacact tttgctgatt gacctgaatg ctgtcatcaa cgtggctgat tgcgccatta
    14821 gggcagctac gggcgcaggg cgcatcttca caatggtgac aggtcactgc actacgttgc
    14881 tgttgatgtt tgataaccgt aattcgggga tgaaaatggt gttggctcag gacatgttgc
    14941 tcatcattgt gagccatgac acaggcgatt tcacaagcat gacaacccag acattgctga
    15001 ctgttggcca taataaaacg attcataacg accttctttt ttggttgtaa aaaccttatt
    15061 ctttatatga gtgttgttat tacccgactt acaggggaat cggcaatgtt catgtgccca
    15121 gaataagtaa ctatttcgct ataaactgtg gcagatcaaa taatcccatc actgactaaa
    15181 ttgtgtttca gctaactgga acattaatga ttttttgtgg agaagacgcg tgtgattgtt
    15241 aaacgacccg tctcggccag tctggcccgg gccttttttt acattgtgct gctgtcgatt
    15301 ctttccacgg gtatcgctct gctaactctg gcgagcagtt tgcgcgacgc tgaggctatc
    15361 aatattgccg gatcgctacg tatgcagagt taccgcctgg gctacgactt gcaaagtggc
    15421 agtccacaac tcaatgcaca tcgccagttg tttcagcagg cactgcattc accggtatta
    15481 accaatctca acgtctggta tgtgccagaa gcagtaaaaa ctcgctatgc gcatctgaat
    15541 gccaactggc tggagatgaa taatcggctc agcaagggcg atttgccgtg gtatcaggcc
    15601 aatattaata attatgttaa tcagatagac ctgttcgtgc tggctttaca gcactacgct
    15661 gaacgcaaaa tgctgctggt ggtggcgatt tccctggctg gcggtatcgg tattttcacg
    15721 ctggtctttt ttactttgcg ccgtatacgc catcaggtgg ttgccccgct gaatcagctg
    15781 gttaccgcca gtcaacgtat tgaacacggg cagttcgact cgccgccgct ggataccagt
    15841 ctgccgaatg agcttggtct gcttgcaaaa acctttaacc agatgtcgag cgagctgcat
    15901 aaattgtacc tttcgctgga ggcgtcagta gaagaaaaga cccgcgatct ccacgaggcc
    15961 aagcgtcgtc tggaggtgtt gtatcaatgt tcccaggcgc tgaacaccag ccagattgat
    16021 gtgcattgtt tccgccatat tttgcagatt gttcgcgaca atgaagcggc tgaatatctg
    16081 gagttaaatg tcggtgaaaa ctggcggatt agcgaagggc aaccaaaccc ggaattgccg
    16141 atgcagattt taccggtgac aatgcaagag acggtttacg gcgaactgca ctggcaaaat
    16201 agtcacgttt catcatcaga accgctgctt aacagtgttt catcgatgct gggacgcggt
    16261 ttgtacttta atcaggcgca gaagcatttt cagcagttat tgttgatgga agaacgtgca
    16321 accatcgccc gcgaattgca cgactcgctg gctcaggtac tttcttactt acgtatccag
    16381 ttgacgttac tgaagcgttc gataccggaa gataacgcca ccgcacaaag tatcatggcc
    16441 gatttttccc aggcgttgaa tgatgcttat cggcagttac gcgagctgtt gactactttc
    16501 cgcctgacgc tgcagcaggc ggatctcccc tccgcgttga gggaaatgct ggatacgtta
    16561 caaaatcaaa ccagcgccaa actgaccctc gactgccgtc tgccaaccct ggcgctggat
    16621 gcgcaaatgc aggtgcattt gttgcaaatt attcgcgaag cggtgctgaa tgcgatgaag
    16681 cacgccaacg ccagcgaaat cgccgtcagt tgcgtcaccg cgccggacgg caatcacacg
    16741 gtttatatcc gtgataacgg gattggtatc ggtgaaccga aagaacccga aggtcattat
    16801 ggtctgaata tcatgcgcga acgcgcggaa cggctgggtg ggacgctgac tttttcacaa
    16861 ccttccggcg gcggcacgtt agtgagtatt agctttcgct ctgcggaggg tgaggaaagt
    16921 cagttgatgt aatgcctcct actgaccaaa gaatacttgc acttaaggtt cagtataaaa
    16981 gggcatgata atttacatta actccttttt ttctccacga ttggctcgta ccttgccgct
    17041 acagtgaagc aagtcaagcc tacaacgata cgcagaaaca cgaggtcctc ttttaatggc
    17101 gaatttcttt attgatcgcc ccatttttgc ctgggtgctg gcaatcctgt tgtgtctgac
    17161 agggaccctg gcgatttttt cattgcccgt tgaacaatac cccgatctcg cgccaccgaa
    17221 tgtgcgagtg accgctaact atcccggcgc atcggcccag acgctggaaa acaccgtgac
    17281 ccaggttatc gagcaaaata tgaccggcct cgataatctc atgtatatgt catctcagag
    17341 cagtggcacc ggtcaggcat ctgtcacttt aagttttaaa gcaggcaccg atccggacga
    17401 agccgtgcag caagtacaaa accagctgca atcagccatg cgaaagttac cgcaggcggt
    17461 gcaaaatcag ggcgtgacgg tgcgtaaaac cggcgatacc aatattctga ccattgcttt
    17521 cgtctctacc gatggttcga tggataaaca ggatattgcc gattatgttg ccagtaatat
    17581 tcaggacccg ttaagccgcg tgaatggcgt cggggatatc gatgcctatg gttcgcaata
    17641 ttccatgcgt atctggctgg acccggcgaa actcaacagt ttccagatga cggctaaaga
    17701 tgtcactgat gccattgagt cacagaacgc gcagattgcg gttgggcaac ttggtggtac
    17761 accttccgtc gataagcagg cgctcaacgc caccattaac gcccagtcac tgctgcaaac
    17821 accagaacag ttccgcgata tcaccttgcg ggtgaatcaa gacggctcag aggtaaggct
    17881 gggcgatgtc gccaccgtcg aaatgggggc ggagaaatac gattatctta gccgcttcaa
    17941 tggtaagcca gcctccgggc tgggggtaaa actggcctcc ggcgcaaacg aaatggcgac
    18001 agcggagctg gtgctcaatc gtctcgacga gctggcgcag tacttcccgc acggactgga
    18061 atacaaggtg gcgtatgaaa ccacctcgtt tgttaaagcc tccattgaag acgtggtgaa
    18121 aacgctgctg gaagctatcg ccctggtttt cctcgttatg tatctgttcc tgcaaaactt
    18181 ccgcgccacg ctgataccca ctatcgccgt gccggtggtg ttgatgggaa ccttctccgt
    18241 actttacgcc ttcggttaca gcgtcaacac cttaaccatg ttcgcgatgg tactggcgat
    18301 cggtctgctg gtggatgacg caatcgtggt ggtggaaaac gtcgaacgta ttatgagtga
    18361 ggaaggactc actcctcgcg aagccacacg taaatcgatg gggcagatcc agggggcact
    18421 ggtcgggatt gcgatggttc tttcggcggt atttgtacca atggccttct tcggcggcac
    18481 caccggtgcc atctatcgcc agttctctat taccattgtt gcggcgatgg tgctgtcagt
    18541 actggtagcg atgatcctca ctccggctct gtgtgccaca ctacttaagc cactgaaaaa
    18601 aggtgagcat cacgggcaaa aaggcttttt tgcctggttt aaccagatgt ttaaccgcaa
    18661 cgccgaacgc tacgaaaaag gggtggcgaa aattctccac cgtagcctgc gctggattgt
    18721 gatttatgtc ctgctgcttg gcggcatggt gttcctgttc ctgcgtttgc cgacgtcgtt
    18781 cttaccgctg gaagaccgtg gcatgtttac tacctcggta caattgccca gcggttcaac
    18841 ccaacaacag accctgaaag tcgttgagca aatcgagaaa tattacttca cccatgaaaa
    18901 agacaacatc atgtcggtgt ttgccactgt tggttctggc cccgggggta acgggcaaaa
    18961 cgtggcgcga atgtttatcc gcctgaaaga ctggagcgaa cgcgacagta agaccggcac
    19021 ctcgtttgcc attatcgagc gtgcaacgaa ggcgtttaac caaattaaag aagctcgcgt
    19081 tatcgccagc agcccgccag caattagcgg tcttggtagt tctgcaggtt ttgatatgga
    19141 gttgcaggac cacgctggag cgggtcacga tgcgctgatg gcagcacgta atcagttgct
    19201 ggcgctggcg gcggaaaacc cggagctaac ccgtgtgcgc cataacggcc tcgacgacag
    19261 tccgcagttg cagattgata tcgaccagcg taaagctcag gcgctgggcg ttgctatcga
    19321 cgatattaac gacacactgc aaaccgcctg gggttcgagc tatgtgaatg actttatgga
    19381 tcgcggtcgc gtgaagaaag tctatgtgca ggcagctgcg ccgtatcgca tgctgccaga
    19441 tgacattaat ctctggtatg tccgaaataa agatggcggc atggtgccct tctctgcttt
    19501 cgcgacctca cgctgggaaa caggctcgcc gcgtctggaa cgctataacg gttattctgc
    19561 ggttgagatt gttggggaag ccgcaccggg ggtcagtacc ggtacggcga tggatattat
    19621 ggaatcgtta gtgaagcagt tgccaaacgg ctttggtctg gagtggacgg cgatgtcgta
    19681 tcaggagcga ctttccggcg cgcaggctcc ggcgctgtac gccatttcct tgctggtggt
    19741 attcctgtgt ctggcggcgt tgtatgaaag ctggtcggtg ccgttctcgg taatgctggt
    19801 cgtgccgctg ggggttatcg gcgcgctgct ggcaacctgg atgcgcgggc tggaaaacga
    19861 cgtttacttc caggtgggcc tgttaacggt cattggttta tcggcgaaaa acgccatcct
    19921 gatcgtcgag tttgctaacg agatgaacca aaaaggccac gacctgtttg aagcgacgct
    19981 ccacgcctgc cgtcagcgtt tacgcccgat tctgatgacc tcgctggcat ttatcttcgg
    20041 cgtattgcca atggcaacca gcacgggtgc cggttccggt ggtcagcatg cggtgggtac
    20101 tggcgtaatg ggcgggatga tttcagctac tattctggct atttacttcg tgccgctgtt
    20161 ctttgtgctg gtgcgccgcc gcttcccgct gaagccgcgc ccggaataag caataaaaaa
    20221 ggcgacatgc caatgtgtcg cctttttcaa ctttccgatt aagaacctgc tcagcgggtt
    20281 cttgctgttt atactttgtc tcgggaatta cttacgaagc ataacttcga taaagtcttt
    20341 ccagttcccc agttcacgtt caatcataac aacctctctt ataattatgg gtattctacg
    20401 gaaacaatat accgtggtga agctaattta ctcgattgct gcgatgacta cctccggggg
    20461 acaaacctta tgtaaatact atggtcctgc ggtgataatt tgtatgtgat acacagcaac
    20521 atttcgagat attcatacgg catctaatac tgatttaatt ctggttaaaa tacagacaga
    20581 taacaagatg aatattctta atgtttacgt taaaaatgtt taa
//
LOCUS       DAZGLJ010000065        20540 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000065 DAZGLJ010000000
VERSION     DAZGLJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.065"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(277..1023)
                     /gene="cutC"
                     /locus_tag="ACHK49_002133"
     CDS             complement(277..1023)
                     /gene="cutC"
                     /locus_tag="ACHK49_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO9262779.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(1037..1603)
                     /gene="yecM"
                     /locus_tag="ACHK49_002134"
     CDS             complement(1037..1603)
                     /gene="yecM"
                     /locus_tag="ACHK49_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO9262780.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1819..3552
                     /gene="argS"
                     /locus_tag="ACHK49_002135"
     CDS             1819..3552
                     /gene="argS"
                     /locus_tag="ACHK49_002135"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO9262781.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYESLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3594..3638)
                     /gene="yecV"
                     /locus_tag="ACHK49_002136"
     CDS             complement(3594..3638)
                     /gene="yecV"
                     /locus_tag="ACHK49_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFO9262782.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3729..4217
                     /gene="yecT"
                     /locus_tag="ACHK49_002137"
     CDS             3729..4217
                     /gene="yecT"
                     /locus_tag="ACHK49_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO9262783.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4337..4729)
                     /gene="flhe"
                     /locus_tag="ACHK49_002138"
     CDS             complement(4337..4729)
                     /gene="flhe"
                     /locus_tag="ACHK49_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO9262784.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4729..6807)
                     /gene="flhA"
                     /locus_tag="ACHK49_002139"
     CDS             complement(4729..6807)
                     /gene="flhA"
                     /locus_tag="ACHK49_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO9262785.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PVEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMELQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHAPRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6800..7948)
                     /gene="flhB"
                     /locus_tag="ACHK49_002140"
     CDS             complement(6800..7948)
                     /gene="flhB"
                     /locus_tag="ACHK49_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO9262786.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8150..8794)
                     /gene="cheZ"
                     /locus_tag="ACHK49_002141"
     CDS             complement(8150..8794)
                     /gene="cheZ"
                     /locus_tag="ACHK49_002141"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO9262787.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8805..9194)
                     /gene="cheY"
                     /locus_tag="ACHK49_002142"
     CDS             complement(8805..9194)
                     /gene="cheY"
                     /locus_tag="ACHK49_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO9262788.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9209..10258)
                     /gene="cheB"
                     /locus_tag="ACHK49_002143"
     CDS             complement(9209..10258)
                     /gene="cheB"
                     /locus_tag="ACHK49_002143"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO9262789.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10261..11121)
                     /gene="cheR"
                     /locus_tag="ACHK49_002144"
     CDS             complement(10261..11121)
                     /gene="cheR"
                     /locus_tag="ACHK49_002144"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO9262790.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRCFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11140..12744)
                     /gene="tap"
                     /locus_tag="ACHK49_002145"
     CDS             complement(11140..12744)
                     /gene="tap"
                     /locus_tag="ACHK49_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO9262791.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNNDLEHQATWLESNQLSDFLTAPVQESQNAF
                     DVNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQP
                     LAIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHI
                     GIAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTA
                     QAGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRG
                     FAVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVD
                     DIMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSR
                     VAVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12790..14451)
                     /gene="tar"
                     /locus_tag="ACHK49_002146"
     CDS             complement(12790..14451)
                     /gene="tar"
                     /locus_tag="ACHK49_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO9262792.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQAPNWETF"
     gene            complement(14596..15099)
                     /gene="cheW"
                     /locus_tag="ACHK49_002147"
     CDS             complement(14596..15099)
                     /gene="cheW"
                     /locus_tag="ACHK49_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO9262793.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(15120..17084)
                     /gene="cheA"
                     /locus_tag="ACHK49_002148"
     CDS             complement(15120..17084)
                     /gene="cheA"
                     /locus_tag="ACHK49_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO9262794.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(17089..18015)
                     /gene="motB"
                     /locus_tag="ACHK49_002149"
     CDS             complement(17089..18015)
                     /gene="motB"
                     /locus_tag="ACHK49_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO9262795.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(18012..18899)
                     /gene="motA"
                     /locus_tag="ACHK49_002150"
     CDS             complement(18012..18899)
                     /gene="motA"
                     /locus_tag="ACHK49_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO9262796.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQMTTEEA"
     gene            complement(19026..19604)
                     /gene="flhC"
                     /locus_tag="ACHK49_002151"
     CDS             complement(19026..19604)
                     /gene="flhC"
                     /locus_tag="ACHK49_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO9262797.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19607..19957)
                     /gene="flhD"
                     /locus_tag="ACHK49_002152"
     CDS             complement(19607..19957)
                     /gene="flhD"
                     /locus_tag="ACHK49_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO9262798.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4972 a         5615 c         4967 g         4986 t
ORIGIN      
        1 atatgattta ttacaatgta atcattaatt gctaaggaat aacccagttg ctatttagaa
       61 tatatattta tcagttttag caatttaaat cccataatca atgtgaatat aaaaaatatt
      121 attagcacaa cgctatgttc actgtcaccc gctcaacaga tcacggttat cgttcgtttt
      181 ttatactgct cagggataaa aaaaggcccc tgttgaaatt gcaggggcct ggtacgagca
      241 agcatcatat tgggcgacat gatgcaacgg taaaaatcat ttggcctgat ggcgttcaat
      301 gattcctttc atttcagcaa ccgccgcccc gtctacgata taacgcgaat actcgtccgc
      361 gtgttcatct gatgacatgg acaatccttg attacgataa cgcatcggtg aggcttgcca
      421 cgctcccgcg gagctatgga cttccagcac tccggcatcg aggaagtggt gcaggttttc
      481 tgcacggact cctgctccgg ccataatgat tggagcatca cgatgggcaa taagttccat
      541 aatttttgat aaaccttgca gtgcgtctga tttttgccct gatgtcagta cccgggcaat
      601 gcccaattcc gcaagattat tgagtgtata taaagggtta gcgcacatat cgaaggcgcg
      661 atgaaaagtc actgccagcg gaccggcagc agccattatt ttttccattc gtggcatatc
      721 gacattcccg tcaacatcga gaacgcccgt caccagtcca ggaaaaccta attcgcggac
      781 cgtgcgcaca tcctcaagaa tggcggcaaa ctcaccgtcg ctgtagcaaa aatcaccacc
      841 gcgtgggcga attatcggat gcacagggat cgtcacccgc tggcgcacgg atttcagtac
      901 ccccaacgac ggcgttaagc ccccctcttt tggggctgcg cataattcaa ctctgtctgc
      961 gccgttttgc tgcgccgtta gtgcacattc catgctgtaa cagcaaattt ccagtaatgc
     1021 catttttgct ccttaattac gctgactgct cgctggcaac gatctcttca atggaccacg
     1081 gatgaaactt aatagttgtt ttgccatcgg tgaccgccag cgtcggattc ggtaatcgct
     1141 catgctcacc ttttggtgaa ctggttttaa ctgagatccc cggtaggctg agtccttcat
     1201 cagaaagcaa tgccagcgca cgggcattca gcgtttctgg atcacccggc agaacgattt
     1261 caatatgttc ccaaccttcg tgtgggtaac gtttttcccc gggccacggt agctccacaa
     1321 tagaaaactg ccagtgcgca acctgtaccg gttcatgcaa tttaaacaga caaatcggtc
     1381 tgccattgat catattttct gacaaaagct cgccacactg ttcaaacccg cgacgccagc
     1441 gttcagcagt ggcgttttga tggcaacgca aagaaatgtg atcggcagtc agcggagtga
     1501 tattcaaccc cagacggagg gaaagttcat ctaatgcgtg ggtaaatcgc ggtaaatccg
     1561 atgcaatatc ctgcagctcg tcgatagatt gccagttcgc cataatcact cttcgtcttt
     1621 cagtaaaagc gttaatttac cctgttgccc tgcgccaacc aaccgctgat ttcacgccgc
     1681 ttctgatgca atagtgaaaa cggcaatacg ccacgcgcac gttgctgacg aaaacagcca
     1741 tttgcagtat actcccgccc taatttcttt aactggtgcg ggcaattttt gctcgcttca
     1801 tcaatgtaag gtattccggt gaatattcag gctcttctct cagaaaaagt ccgtcaggcc
     1861 atgattgcgg caggcgcgcc tgcggattgc gaaccgcagg ttcgtcagtc agcaaaagtt
     1921 cagttcggcg actatcaggc taacggcatg atggcagttg ctaaaaaact gggtatggca
     1981 ccgcgacaat tagcagagca ggtgctgact catctggatc ttaacggtat cgccagcaaa
     2041 gttgagatcg ccggtccagg ctttatcaac attttccttg atccggcatt cctggctgaa
     2101 catgttcagc aggcgctggc gtccgatcgt ctcggtgttt ctacgccaga aaaacagacc
     2161 attgtggttg actactctgc gccaaacgtg gcgaaagaga tgcatgtcgg tcacctgcgc
     2221 tctaccatta ttggtgacgc agcagtgcgt actctggagt tcctcggtca caaagtgatt
     2281 cgcgcaaacc acgtcggcga ctggggcact cagttcggta tgctgattgc atggctggaa
     2341 aagcagcagc aggaaaacgc cggtgaaatg gagctggctg accttgaagg tttctaccgc
     2401 gatgcgaaaa agcattacga cgaagatgaa gagttcgccg agcgtgcacg taactacgtg
     2461 gtaaaactgc aaagcggtga cgaatatttc cgcgagatgt ggcgcaaact ggtcgacatc
     2521 accatgacgc agaaccagat cacttacgat cgtctcaacg tgacgctgac ccgtgatgac
     2581 gtgatgggcg aaagcctcta caacccaatg ctgccaggaa ttgtggcgga tctcaaagcc
     2641 aaaggtctgg cagtagaaag cgaaggggcg accgtcgtat tccttgatga gtttaaaaac
     2701 aaggaaggcg aaccgatggg cgtgatcatt cagaagaaag atggcggcta tctctacacc
     2761 accactgata tcgcctgtgc gaaatatcgt tatgaatcac tgcatgccga tcgcgtgctg
     2821 tattacatcg actcccgtca gcatcaacac ctaatgcagg catgggcgat cgtccgtaaa
     2881 gcaggctatg taccggaatc cgtaccgctg gaacaccaca tgttcggcat gatgctgggt
     2941 aaagacggca aaccgttcaa aacccgcgcg ggtggtacag tgaaactggc cgatctgctg
     3001 gatgaagccc tggaacgtgc acgccgtctg gtggcagaaa agaacccgga tatgccagct
     3061 gacgagctgg aaaaactggc taacgcggtt ggtattggtg cggtgaaata tgcggatctc
     3121 tccaaaaacc gcaccacgga ctacatcttc gactgggaca acatgctggc gtttgagggt
     3181 aataccgcgc catacatgca gtatgcttac acgcgtgtat tgtccgtgtt ccgtaaggca
     3241 gaaattaacg aagagcaact ggctgcagct ccggtaatca tccgtgaaga tcgtgaagcg
     3301 caactggcag ctcgcctgct gcagtttgaa gaaaccctca ccgtggttgc ccgtgaaggc
     3361 acgccgcatg tgatgtgtgc ttacctgtac gatctggctg gtctgttctc tggcttctac
     3421 gagcactgcc cgatcctcag cgcagaaaac gaagaagtgc gtaacagccg tctgaaactg
     3481 gcacaactga cagcgaagac gctaaagctg ggtctggata cgctgggtat tgagacagta
     3541 gagcgtatgt aatcgatttt tcgtgagagt aaagcctgat cagggttacc tgatcaggct
     3601 tttttattgc catctaaatg tattctgaaa atggacatgc cattgttttc tgactgttgg
     3661 ataagaggcc agaagcgtaa tatccggccc cagggaaacg ataacggttg aatttaagga
     3721 ataccgcagt gtttaaattt cttgtattaa cattaggcat tatctcttgc caggcttacg
     3781 cagaagatac ggttatagta aacgaccatg acatttcagc catcaaagat tgttggcaaa
     3841 aaaattcaga tgatgatact gacattaacg tgatcaaatc atgcctgcga caagaataca
     3901 atctcgtcga tgcgcaatta aataaagcct atggtgaagc ttatcgttat atagaacaag
     3961 tgccacgcac aggtgtaaaa aaacctgata ccgaacaact taacttgctt aaaaaatcac
     4021 agcgagcctg gctggatttt agggacaaag aatgtgaatt aatcctttca aatgaggacg
     4081 ttcaggattt aagtgaccct tattctgaat cagaatggct ctcatgtatg atcatacaga
     4141 ccaatacgcg aactcgccag ttgcagctat accgtaactc tgaagatttt tatccaagcc
     4201 ctttgacaag aggataattc acatcttttt ggcatgtttt gttgcaagct attcctgata
     4261 aataattgca acaagacatc gagccttttt cactgagtta ttaaacatac tcgcgagcgc
     4321 gtaatttttt tgtccttcag cgataattca caatcacttc attacgttgt acctttagcg
     4381 gtggaattaa ccgaccaccg cctggcactt cccagataaa tcgcaacggt tctgctgccg
     4441 ctataccgga aaaggccacg gtggttccgc tctgcccctc taattcgaca caacgagatt
     4501 gcgaacacaa gcgaacccgc agtcctgaag gtgtcgggcc gataagctga taacgccacg
     4561 ctaccagcgt catcaatcct gaagcaggtt gtcgcgtaga aagaggcgca gacgacatcg
     4621 actcaccgcg atgatttagc gtaattccta cactacttgc ctgccacatc ccctccccgg
     4681 cggcttgcac cagcagcgga aacaataata ttgctaataa ggttctcatt atttgccgcc
     4741 aattgtcgcc gtcatgcgga tatgtcggtt atcagacagt tccagattcg acaggaccac
     4801 taactgcggc aagctgcggc gcaggaagcg agacaataat ggtcgcggcg cgtggttcac
     4861 caacaatact ggcggcgcac ccagcatctc ctgacgggat agcgcttcct gagtttgcgc
     4921 cagtaaacga tccgccagcc ctggctccag tcctcccccg ccctgcagcg cctgtagtaa
     4981 caaacgttcc agcggtgtat cgaggccaat aacatggact tcatctttgc caggaaacca
     5041 ctgctgggta atcgcccgtc ccaacgccac gcgcacgacg gcggttaatt catgtggatc
     5101 gctttggatg ggcgcatgtt ccgccagcgt ttcgagaatg gtgcgcatat cgcgaatcgg
     5161 tactttttca tcgaggagat tttgcagcac tttatgcagt gtggtgagcg tgacgacgcc
     5221 aggaacgaga tcttccgtca gctttggcat ctcctgggcg acgcgatcca acagctgttg
     5281 cgcctcctga cgaccaaaca gctctgcggc atgctggcta atgaggtggt taagatgcgt
     5341 tgctaccacc gtgctggcct caaccactgt gtacccctga atctgcgcct gttcttttag
     5401 cgcactttca atccagatag cattcaggcc aaatgccgga tcgacggtcg cctcaccagg
     5461 taacgtcccg gcagcggttc cagggttaat cgccagccag cgccccggat aagcatcacc
     5521 actgccaatc tccacgcctt tcatcaaaat gcgatagcgg gcaggttgca gctccatatt
     5581 gtcgcgaatg tgcaccactg gcggcagaaa tcccatctcc tgggcaaatt tcttgcggat
     5641 actgcgtata cggcccaaca actcaccatc ctgctggaaa tcgaccatcg ggatcagtcg
     5701 ataacccact tccattccca gagaatcttc cagttgtaca tcgttccacg tcgcttcgac
     5761 aacggtatta ttctctgcca tttttaccgg tttgggttcg acaggcgctt tttgttcgcg
     5821 tccgcgtatc caccaggcca gccccagcaa tccggcagtg aacagcaaaa ataccaggtt
     5881 cggcattcca ggcaccaggc cgagtaaacc gagcacggcg gcgcttaaca acataacgct
     5941 tgggttactg aaaagctgat tcaccatctg ctcgccaaca tcctgatcgg tgctgacacg
     6001 cgtaacgatg acccccgcgg cggtagaaat caccagcgcc ggaatttgtg ccaccagacc
     6061 gtcgccaatg gtcaatagcg tataactttc cgccgcgtgt cccatgctca tgccatgttg
     6121 cagcacgccg accagcaacc cgccgacaac gttaatgacc atgatgagga tcccggcgat
     6181 ggcatcgccg cgaacaaact tacttgcccc gtccattgag ccgtaaaaat cggcttcctg
     6241 agtcacttcg gagcggcgtt ttttcgcctc atcttcacca atcaatccag cgttaaggtc
     6301 ggcgtcaatc gccatctgct tacccggcat accatcgaga acaaagcgcg cacccacttc
     6361 tgcgatacgc ccggcacctt tggtaatgac cataaagttg atgatcacga gaatgacaaa
     6421 cactacgata ccgatagcga aattgccacc aacgaggaag tgaccgaatg cttcgaccac
     6481 cttcccagcc gccgccgcgc cggtatgccc ttccatcaaa atgatacggg ttgaagccac
     6541 gttaagtgcc agacgcaaca acgtagtaaa caacagaatg gtcggaaacg cagcaaactc
     6601 aagcgtgcgc tgggtaaaca tcgccaccag caacaccatg atcgacaagg caatattgaa
     6661 ggtaaacaac aggtcgagta tgaatgcggg cagtggcagc accatcatcg acaagatcaa
     6721 caggatcaaa atcggtccgg caaggatctg ccattgtgtc gatttcaggt ttgcgggcag
     6781 gcgcagcatc gcggccagat tactcatggg tcggtttctc gttaataaaa tccagggctt
     6841 ccggcaccgg aagatgagta ggttgtacag ggcgctgtcc accagccaga cgccagcgtt
     6901 tcagttgcca gacccaggcc agcacttccg ccaccgcggc gtacagttga cccgggattt
     6961 gttgaccaat ctccgcatgt cgatacagcg ctcgcgccag cggcggcgct tcaagcgtcg
     7021 ggacgttatt ttcagcgcca atttcacgaa tgcgcagcgc gaccagccct gcacctttag
     7081 cgaccacttt cggtgcgctc attttgtttt cgtcatactg caacgctacc gaatagtggg
     7141 tcgggttatt gacaatgaca tccgctttcg gcacatcggc catcatccga cgccgtgcag
     7201 cagctcgctg catctgacgg atccgccctt taacatgagg gtcaccttcg ctttgtttga
     7261 actcatcacg aatatcctgc cgtgacatac gcagcttttt caggtggctg aatatttgga
     7321 aaaagacgtc aaatcccacc attggaatga caccaagcac caccagcagt gcgcatagcc
     7381 ctaccaaatc catcgcatta cccatggcgg taatcggaga ctcggccatc aagcgcatca
     7441 tctgcggcca gtgatgccag agaaaaaacc ccgtcacgct gccaaccagg atggttttca
     7501 aaattgcttt aagcaactcc gcgccagtct gagccgagaa catccgttta atgcccggta
     7561 gcgggttgag tttggaaaac ttcggctgca aggatttgcc gctaaatacc agccctccca
     7621 gcatgaccgg agaaatgagc gccaccagca ccacgccgct aatcagcggc agcagcgcca
     7681 gcatggcttc tctgatcagc agaataatct gcccgaggat cagattcggg tcattgataa
     7741 tactgtgatc aaaatgcagc ccagcggaga gcatgcccga caatcgacgg gccagcgaca
     7801 caccgccaaa ccagataaca ctaacgccca ccagcaaaat cagcagtgag gtcagttcac
     7861 gggaacgcgg gatttgcccc tcttcccgcg ctttttctag tcggtgaggt gtgggggctt
     7921 ctgttttgtc gtcgctctcg tcagacacgt cgccaatcct ggaaagagta ttaagcgtga
     7981 atgatgccag agcgcaaagc gttcaatggt ttgagtaagg ggcaaaacag gcgggattta
     8041 gggcttttgc tgccacacat caagcatagt gtgcgtttgt cggatgcggc gtcatcgcct
     8101 tatcagaccg cctgatatga cgtggtcacg ccacatcagg caatacaaat caaaatccaa
     8161 gactatccaa caaatcgtcc acctgatcct gactggctac cacaccggct ttgctggtat
     8221 cgacctgagg tccattaagc aaactctggt tttcacgttt tggacgcgac tcctgttccg
     8281 ggatgttttc caacagcacc atcagcaact ggcgttcgat ctcctgaatg acatccatca
     8341 tccgcttaat gacctgcccg gtgagatcct gaaaatcctg cgccatcatg atttccagca
     8401 gttgcgcgtt agtaaagctg gtatgcgcgg gtacatctgc cagaaattgt cgtgtatctg
     8461 ttaccagttc acgggcgtcg gcaaggtcaa tcggatcggc aaaccagtca tcccaacgtt
     8521 gggttaacgc ttttgctgat ttctccattt gatcctgatg cggttgtgac gcctcaacac
     8581 tgttcagcgc ccgctccgca gcctgggcgg tcatctgcac aacatagtac aaacgatcgc
     8641 gcgcatcggg gatggcttcc gccgcttcgg caatggcctg atccagcccc agttcccgca
     8701 aactgtcgcg cagcatacgc gtcaggctgc cgatgcgcgc aatgatatcg ccagctgaat
     8761 gctcgtcagc aggtttgatt gatggttgca tcatagtcgc atcctcacat gcccagtttc
     8821 tcaaagattt tgttgagttt ttcctccagc gtcgcggcgg taaatggctt caccacatag
     8881 ccactggccc ccgcttgcgc cgcagcaatg atgttctctt tcttcgcttc tgcagtcacc
     8941 attaacactg gcaatgccga catcgcgcca tccgcacgaa ttgttttcag caattccagg
     9001 ccatccatat tgggcatgtt ccagtcggag ataacaaatc cataaccgcc tgcctgcaac
     9061 ttattgagag cgtcgacgcc atcttccgct tcctcaacat tattgaatcc cagctctttc
     9121 agcaggttac gcactatgcg tcgcatggtg gaaaagtcat ccacaaccaa aaatttaagt
     9181 tctttatccg ccatttcaca ctcctgattt aaatacgtat cgcctgtccg gcactaattt
     9241 ttgccaacat ttgctggctt acctggctaa gatcgaccac ttcgcagaca ccacccatat
     9301 tgatggcctc gcgcggcatg ccgaacacca cgcaacttgc ttcgttttgc gcaagggtcc
     9361 atgcccccgc ctgacgcatc gccaacattc ccgccgcgcc gtcgttgccc ataccggtca
     9421 ggatcacccc aaccgcatta cgccccgcct gtttggcgac agaatggaac aacacatcta
     9481 ccgaaggccg atgacggtta accgccgggc catcgtgaat tttgatttgg taatttgcgc
     9541 cactacgcga cagctccata tgccgatcgc ccggcgcaat ataggcatgc cccggcaaga
     9601 cacgttctcc gtcttcggct tctttaaccc cgatctggca aagcttatta agtctgtcgg
     9661 caaaagagcg ggtgaaaccg ggcggcatat gttgggtaat taacaatgcc gggctggaaa
     9721 gcggcaacgg ttgcagtacg tgacgaattg cctcagttcc acccgttgaa gcaccaatcg
     9781 caatcagttt ttcagaactc aacaacggac ccgccttcag cgttgtcggt gctgacaatg
     9841 gcttatgtgc tgcaaggctc gcctttgctg ccgtacgcac cttttcagca atcatttcgc
     9901 tatacgccag cattccttcg cgaataccca gttgcggttt ggtgacaaaa tctattgccc
     9961 ccagctccag cgcgcgcagc gtgacttctg atcctttgcc ggtcaaggaa gaaaccatca
    10021 caacgggcat tggacgcaaa cgcattaatt tttcgaggaa atccagtccg tccatccgcg
    10081 gcatttcaac atccagcgtc agcacatcgg gattgaattt cttaatcaag tcacgcgcga
    10141 ccagcggatc aggcgcggtc gccaccattt ccatgtcgct atggctgttg atgatttctg
    10201 tcatgatctg gcgcatcagt gccgaatcat cgacagataa caccctgatt ttgctcatcg
    10261 ttaatcctta cttagcgcat acaccgtctg accacgcagc gtgaagcggc gctcaaggtg
    10321 gctaaagttt tcagagtgac ccgcaaacag caatccgtcg ggtttaagga gcggaacaaa
    10381 gcagcgcaaa atctcctgct gggtattttg atcgaagtag atcatgacgt tacgacagaa
    10441 gatcgcatca aacggccccg gcacggtgta ctgtttcgcc agtagattca gcggggcaaa
    10501 atcaacatag ttcgccagct cctgacgcac gcgtaccagc ccttcatgcg gtcccgtccc
    10561 tcgcatgaaa taccgttgca gttgctgcgg cgtcaggttt ttcaactctt catggcgata
    10621 aataccgctt ctggcttttt ccagcacttc ggtgtcgata tcactggcaa acactttcca
    10681 gcgtccgggc gcggtgccca atgtgtcagc cagcgtcatc gcaatgctgt acggctcttc
    10741 gccggtcgaa gccgccgcgc tccatacgcg atactcgcca gaacggcgac gtgcgtgatc
    10801 cgcgagcaga gggaaatggt gtgcctcacg gaaaaatgcc gtcagattcg tggtcagcga
    10861 attgataaac gcctgccact caccgctgtg ctgattagat tccagcaagt tcagataatg
    10921 accgaaatcc gtcagtccca gcgaacgcaa acgacgaacc agtcggttgt aaaccatgtc
    10981 gcgtttatgg tcagccagaa cgatcccggc tcgttgatag atcaattgac ttatccgccg
    11041 aaaatgcgcg tcggaaagcg ccaggcgctc ggtcatctgt aacaataaag acgtttgccc
    11101 acagggcaga gatgaagtca tagcgccttc tcaatcactt caggatacca ctggcgcaat
    11161 ttgtaactgc gccgactcat gtcgtgctac ttcatgttct tcaagggtaa ataccgccac
    11221 acgcgacgaa agatggtcgg cctgattcgc cagttgttcc gttgccaccg ccgcttcttc
    11281 taccagcgag gcgttctgct gagtcacctg atccatctgg ctgacagcct gtgcaacctg
    11341 ctcaatcccc cgctgttgtt cctccgacgc agaggcaatt tctcccataa tgtcgtccac
    11401 gcgggtcacc gaactgacaa tatcgatcat ggtcgcggcg gcgttattca ccagtttcga
    11461 accctgctgg acacgattga ctgactcttc gatcagccct ttgatctctt ttgctgcctg
    11521 cgcgctacgg ctggcaagat tgcgcacttc acctgccact accgcaaaac cacgcccctg
    11581 ctctccggcg cgagccgctt ccactgccgc attcagggcc agaatattgg tctggaaagc
    11641 aattccgtcg ataacgctga taatgtcgcc aattttttgc gagctggtgg cgatctcctg
    11701 catggtgtga gtcatggtac tgacctgaac accgcccgcc tgcgccgttg tcgcggcatt
    11761 ttttgccagt tccgacgcct gtcgtgcgtt atcggcgttt tgccctaccg tggcggttaa
    11821 ttgctccata ctggcggccg tttgtgccag cgatgccgcc tgctgttcgg tacgacttga
    11881 gagatcgtta ttgcctgcga caatctccgc gataccaatg tgcatctcct ggcttccctt
    11941 acgcacatca ctcaccgtcc cacgcaaagc ctgctgcatg gtcttcaggc tggcaaaaat
    12001 ggcggtgatc tcattacgac catataccgc aatcggacgc gccagattac ccgcagcaat
    12061 gctgtcaaaa tggctaccga taatggccag tggttgaaca atcatcttgc gcgtccacca
    12121 cagcgcactg ctgatgtaga tcgccgcaac aataatcatg ctgataaaca ccagcgccga
    12181 aatctgatag ttacgctggc tttgcgcact ggcggcttcc agcacatggt tgatctccag
    12241 ctgccaggcc tcaaagttaa cgtcaaacgc attctgtgat tcctgcaccg gcgcagtgag
    12301 gaaatccgaa agttgattac tttcaagcca ggtcgcctga tgttcgagat cgttgttgtg
    12361 ccagcgggca aaacttttct ccgtctcttt ttgcaacgcc ctgacgtgct cgttgcccgc
    12421 agtcatcgcc ataaaacttt taaacagcgt ggtggattgc gtcagactgg cgcgcgccgt
    12481 cgtcatcaac gttttaatgt catccgccgg atagctaagt gccgtcagag tgcccgcttt
    12541 gttcagcgcg gtactggcct gtaacattac cgcccgagtt tgcgctaatg ccgcacgttg
    12601 ctgattgctc tgctcgacct gattcagtcg ttgcaaatcg tcgcgaaatg cccaaaaaga
    12661 catgccgtta ctgccaatct gcaagatccc gcagagaatc aaaattaaaa acagcgtggt
    12721 cgaaattcga atacgattaa acatcaacgc tccccatcag gcggcaatga ccgcgttagt
    12781 aaatactcgt caaaatgttt cccagtttgg agcttgttca gcaattcgca gtcgtggctg
    12841 tgccggtggt tgctcactgg caggacggga tggtgtttgc ggtttattgg tgagtgggct
    12901 ggctgccaga cggaacgcgg aaaccgcttg cgttaaacga ctcgcctgtt cttccagcgc
    12961 agcggcggcg gcagctgatt cctgcaccag cgatgcgttc tgttgcgtga cgcgatccat
    13021 ttccgaaacc gccaatgcga cttgatcgat gccacggctc tgttcatccg atgccgatgc
    13081 aatctcgccc ataatgtcag tcacgcgagt gacagcattg acgatattgt tcattgtttc
    13141 cccggcgctt tcgaccagca ccgaaccggt atcaacgcgt gagacggagt cttcaatgag
    13201 ggctttgatc tcttttgccg cctgggcgct gcgactggca agattacgca cttcacccgc
    13261 caccacggca aaaccacggc cctgttcacc cgcacgcgcg gcttcaaccg cggcattcag
    13321 cgcgaggata ttagtctgga aggcaatacc gtcgataacg ctgataatgt cggcaatttt
    13381 cttcgaacta tcggcgatct catgcatcgt tttcactacg ccatccacca ctttgccgcc
    13441 gtgctgggcg gtgtcggagg cactttgcgc cagttgcgag gcctggcggg cgttatcggc
    13501 gttttgcttc actgtcgcgg tgagctgctc catgctggcg gcagtttctt ccagcgcgga
    13561 tgcctgctgt tcagtacggg aggaaagatc ggtgttgccc gccgcaattt cacgggtacc
    13621 ggcatagatg gcatctgaac cttcgcggac atgagtgacg gtgtcagtca aagagcgttg
    13681 catatgtgaa acgctctgcg ccaggtcgcc catttcactg cgcccgtcaa tggtcagggt
    13741 attcgccagg ttaccaccgg cgatttcgcg aatgtgagca ataatttttg ccagcggagt
    13801 aagcaacata cggcgaatgc cgtaccacgc caccagcaga atcaatacca ccaccagcgc
    13861 gataaccgcc agttgccact gggcaaatcg gtaatcatct gcgttgtcag tgacaatatc
    13921 gcgatacagt ttttcactgc tgagggcgta ctgagcaaac gcttcgccca ttgcattttg
    13981 cattccctgg gttggctgag cgaaataagc tccagtattg ccataatcaa gataatcaat
    14041 cagttcagtt aacgctgtgt gatagttttt atatttttca tcaatattac gactggtagc
    14101 gaccatttca ggtaacggtg ccatgctttt gaatttttta taatgagtcg cggcctgggc
    14161 caatgttttc ctggcgctat cgagcaattc aactttggcg ttactttgtt gattggagga
    14221 atccatcatc atccgcaccg ctgaacgact caggttaatg cgcgtttgca gcattaaatc
    14281 ccaggttgac gtcagctcgc cctgctgttc ccgtaattga ttggaaacca caaagctctt
    14341 ctggctatgg tgaagggaag aaaaaaacag actgccggaa ataagctgta acagtgcgaa
    14401 tacccccagc accattacca acagcgtgac tacgcggata cggttaatca taaggcacct
    14461 tcctgaaaac aagttaatct cgttatcggc aagaaggggg gaaactttat tgctgatgcc
    14521 acccgccgcg aaattgaaat aaaaaacccg atgcgcagat catcgggttc atttcaattg
    14581 aggaaatcgg gagaattacg ccacttctga cgccgcgcta tctaacagcg ccatctcttc
    14641 gctgttcagc agtttttcga tgttcaccag aatcaacatc cggtcgccca gtgcgcccag
    14701 tccagtgaga tattctgttg aaagcgtcac ggcaaactct ggtgccggac gaatttgctc
    14761 cgccgtcaat gaaagcacgt ctgaaacgcc gtcaaccacg atgccgacca cccgctgtcc
    14821 gagattcagg acgataacta ccgtgttgtc gttatagtcc acatccacct ggctgaactt
    14881 aattcgtaag tcaacaatcg gcacaataac gccgcgcaga ttcgtgacgc ctttgataaa
    14941 cgctggcgtg ttcgcaatcc gtgttacctg atcgtagcca cggatctcct gcactttcag
    15001 gatatcaata ccgtactctt catcaccaag ggtaaatacc agaaattcct ggcctgacgg
    15061 ctcgctggcc agctttgtta cattcgtcat accggtcata ttgttacctt tttactcatt
    15121 caggcggcgg tgttcgccat acgttgttcg cggtttatcg cctgcaaggc ggagacatca
    15181 acaatcagtg ccacgctgcc gtcgccaaga atggtcgcag cagaaatgcc ggggactttg
    15241 cgatagttac tttcaaggtt tttaaccaca acctggtgct gaccaattaa ttgatccacc
    15301 agcaaggcgt agcggcgacc gccactttgt aagatcacca caattccctg ggtggcttcg
    15361 gttttcgcgc ccgcgacgtt gaacactttc cacagttcga cgatgggcag atattcaccc
    15421 cgcacttcca gcacccgctc gccgccggcc agtggatgca gatcggcttc acggggttgc
    15481 agtgattcca taacagcatt cagcggcaga atgaaaactt catccgcaac gcgtacggac
    15541 atgccgtcga ggatggccag cgtcagcggc agtaaaatgc ggatcgtagt gccagtaccc
    15601 tgcttcgact ggatttcgac atgaccgccc atctcctgga tattacgttt aacgacgtcc
    15661 atgccgacgc cgcgcccgga gacgtcggtg acctgctctg ccgtggagaa gcctggtgca
    15721 aatatcagca tcgcgacttc gtcgtcgctc atgttttcgc tgacagtcaa accttgcgag
    15781 gccgcttttg ccagaattcg ctcacggttt agccccgccc catcgtcggt cacttcaatg
    15841 caaatgttgc cgccctgatg ttcggcagac agaattaaat ttccgacgct gtttttacct
    15901 gcggcgagcc gtttttctgg cagttcaata ccgtgatcga ggctattgcg taccaggtgg
    15961 gtcagcgggt cgataatgcg ttctatcagg cttttgtcga gttcagtaga actgcccacc
    16021 agcgtcagtt ctacctgctt gccgagtttt cccgccagat cacgcaccag ccggggatag
    16081 cgactaaaaa catattccat cggcatcatg cgaatcgaca tcactgattc ctgcaaatca
    16141 cgggcgttac gttgtaactg ccccatgctg gttatcaaat caccatgatt aaccgggtcc
    16201 agttcgctgg aacgctgggc aagcatggac tgggtgataa ccagctcgcc gacgaggtta
    16261 attaattgat caaccttttc taccgctaca cggatgctgg tggattcatt gctgcgcgtc
    16321 gttttttccc gctccacgcg gccggttggc gcttgttcgg ctgccagttt aagcactggt
    16381 ggggtggata tttttggcga gacttctact gtttcaaagg taatctgatc ggcttcaatc
    16441 acaaaacaga gtaccgctgt gatgtcatct tcggcgatgt cgcccggtaa tattgccgag
    16501 agcgaatccg cccctttcac cacgtcagtt aacgttgtca gatgtcccag ttcttcttcc
    16561 agcaggtcga cttccccggc cttcaggcgc gaaaggataa ttcgtcgcgg cgactgactg
    16621 cgactctgct catcttgcgg ttcacttttg gcaaccacac ttaatcgggt cactgcggat
    16681 ggcgtttcgc ctttcgcttc taatgccagt tgacgcaagg cctggcagat ataatcgaag
    16741 ctggcggcat ccggctcttg cgactgttta taagcgtcga gctgttcttg catgatgtcc
    16801 ttcgtttcca aaaacagatt gataatgtcg gtgttgagtt gcatctcacc tcgtctggct
    16861 tcatcgagca ggttttccat cagatgcgtg gtttcctgca aaacgctgaa gccaaaagtt
    16921 cctgcccctc ctttgatcga gtgggcagcc cgaaagatgg cattcaattg ttcggcatct
    16981 ggcgcttccg gctgcaaaac cagcagatgc tgctccatgt cagccaacag ttcgtccgct
    17041 tcatcaaaaa atgtctgata aaaatcgctt atatccatgc tcacgctgtc acctcggttc
    17101 ggctgatggc attgtgggaa cactgacctg tggtgcaacc tcaggttttt ccagggcgct
    17161 tactggctca ttctggcttt cggcgttttc atgcaaaatg gcctgttcgg cttgtttgtt
    17221 cagtaccagc aggctgatgc gacggttgac ggcatcatca ggtccgcgat cgcttaagcg
    17281 catcgttgcc gccatgccga cgacacgtaa cactttgccg ctatccaacc ctccgaccat
    17341 cagttcgcgg cgggatgcat tggcccgatc ggcagaaagc tcccagttgc tatatccttt
    17401 ctcaccgctg gcgtagggga aatcatcggt atgacctgaa aggctaatac ggttgggaat
    17461 accgttcagt acaggcgcaa tggcacgcag aatgtcgcgc atatagggtt cgacatcggc
    17521 actgccggtt ctaaacatcg ggcgattctg gctatcgatg atctgaatac gtagaccttc
    17581 ctggaccaga tcgattttga gatggggacg taacgcccgc agtttcggat cggactctat
    17641 caactggtcg agatcacccc gcaatttccg caatcgactt tgctccatgc gttttttcag
    17701 ctcttcgatg ttcggctgct tattcacttc cccctggctt tgggtgtaat catcaccacc
    17761 gccgggaatt gggctttcac tattagaaat gcgatcgccg cccgtaaccg cagtcgccag
    17821 tggagtccgg aagtactccg caatctgaat cagctctttt gggctggaga tggagatcag
    17881 ccacatcacc agaaaaaagg ccatcatcgc agtcataaag tcggcataag caatcttcca
    17941 cgatccatgt gctgccccgt ggcttttggc tttgcgtcgt ttgacgacaa taatcggatg
    18001 cgcttgattc ttcatgcttc ctcggttgtc atctgttgtt gcggattttt caccgcacgc
    18061 acatgctctt ccagttcaat aaacgacgga cgttcgctgg aatagagcgt tttgcgacca
    18121 aactcaacgg cgataggcgg tgcgtaaccg ttcagattag aaagcagagt gactttgacg
    18181 cactgcatca ttttgctggt ttcggcgctt ttctgacgta aaacactcgc taatggggaa
    18241 ataaatccgt aagccaataa aatgccgagg aaagtcccca ccatcgcatg tgcgataagc
    18301 gcacccagct cggcggcagg acgatcggct gaacctaacg cgtgaacgac ccccattaca
    18361 gccgcgacaa taccaaacgc cggaagtgag tccccgacca gcgccagact gttcgccggg
    18421 acttctgcct cgctttcgtg cgtctcaatc tcttcatcca tcagggcttc gatttcgaag
    18481 gtgttcatat gaccgctgat aatcagacgc agataatcga cgataaaatc aagcatgacg
    18541 ctatccgcga ggatgcgtgg gtagctggcg aagatctcgc tctcacgggg attttcaata
    18601 tcacgttcca gcgaaaacat ccccatctgc cgcgatttcg ccatcaaccg ataaagcaga
    18661 gccagcagat ccatatacat tgctttggtg tatttggagc gacgaaacag caacggcagc
    18721 gccttcagcg tgcctttaat cgctttgcca ttattgccga cgataaacga cccaatccct
    18781 gcaccggcaa taatcaccag ttcagcgggt tgatagagtg ctccaaggct tccaccggtc
    18841 atcaaataac cgccgaaaac tgtaccgaga acaaccaggt aacctaataa gataagcacg
    18901 acatcatcct tccactgttg accatgacag gatgttcagt cgtcaggcgt taacgcgcga
    18961 ttggggcaaa aaaaagcagc ggtacgtcgt taccgctgct ggaatgttgc gcctcaccgt
    19021 atcagttaaa cagcctgtac tctctgttca tccagcagtt gtgggataat atcggcagga
    19081 ttctgggaaa gtttacgtct ttttactgcc cgggatggcg gttgacataa gctgcaggca
    19141 aagctgccaa caggctggtg agcgtgggta ataaaattgc cgccgcagca gttgcagctg
    19201 gaaagttgca gtaatccact ttcaacaaac cgcaccaatg tccaggcacg ggttaatgcc
    19261 agcagtggtc cttcttctgc ttgtgggcac tgttcaaggt ataaacggta ggctttgatc
    19321 accgcatcga cgccattaca caaaccggtt ttcagtaaaa actgccatgc attacagaac
    19381 atcgaagcat gaacgttttg ttcccaggtc ataaaccagt cggttgagaa tggcagcatg
    19441 cctttcggcg gtgggcttcc gcgcagttct ttataaagtt ttatcaggcg tccgcgactt
    19501 aactgtgttt cgctttccag catctgcaaa cgagcgccca gggtgatcaa ttccattgcc
    19561 agctgaatat cccgcgcttc ctgaacaatg cttttttcac tcatgatcag gcccttttct
    19621 tgcgcagcgc ttcttcaggc tgattaacat cattcagcaa gcgtgttgag agcatgatgc
    19681 cggtatgaat ttgctggaga tcgtcaacgc gggaatcttg cgtcaactga gtaatcgtct
    19741 ggtggctgtc aaaacggaag tgacaaacca gttgattggt ttctgccagc ttaaccattt
    19801 gcggaagagt cagtgccgct aacgttgtcg ccatttcttc atttatgccg agacgaaaca
    19861 tagcggacgc tttgtcctga acaatcaaac gctgtgcaag tagtaaatat gacaagttga
    19921 tgtcataaat gtgtttcagc aactcggagg tatgcattat tcccacccag aataaccaac
    19981 tttattttta tgcggtttca ccgcaccccg tgatgtcgcc gggaagcccc ggtaaaaaat
    20041 aattagcatt agaatagttg cgataagctg caataagcag aaccaccttt ttggtttaat
    20101 atgtccttac aaatagaaat gggtctttac acttatctaa gatttttcct aaatcgacgc
    20161 aactgtactc gtcactacac gcacatacaa cggagggggg ctgcgatttt caataatgtg
    20221 tgatgcagat cacacaaaac actcaattac ttaacataaa tgtataagtc atacttttgt
    20281 tttgggtgta tttcaatctg ttaaaaagtt tttcgctacg ctagcaagca aaaatgaaac
    20341 aggaataatc gaaatgggat gttgcgcaca gttaaaataa ctcaccgtaa ataatcatct
    20401 gctataaata atcactttca tgcaatacca gataagctat ttttaaacag acacttaccg
    20461 cacaacaaac taattaaata caaagaaagg caacaatcaa cttaatgtta atgaaatgtt
    20521 ttacatatat taaccacttg
//
LOCUS       DAZGLJ010000066        20449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000066 DAZGLJ010000000
VERSION     DAZGLJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.066"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1316)
                     /gene="plaP"
                     /locus_tag="ACHK49_002153"
     CDS             complement(<1..1316)
                     /gene="plaP"
                     /locus_tag="ACHK49_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO9262799.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVT"
     gene            complement(1306..1368)
                     /gene="yoeI"
                     /locus_tag="ACHK49_002154"
     CDS             complement(1306..1368)
                     /gene="yoeI"
                     /locus_tag="ACHK49_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO9262800.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1583..2512)
                     /gene="yeeY"
                     /locus_tag="ACHK49_002155"
     CDS             complement(1583..2512)
                     /gene="yeeY"
                     /locus_tag="ACHK49_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9262801.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2558..3382)
                     /gene="yeeZ"
                     /locus_tag="ACHK49_002156"
     CDS             complement(2558..3382)
                     /gene="yeeZ"
                     /locus_tag="ACHK49_002156"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9262802.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3465..3719)
                     /gene="yoeB"
                     /locus_tag="ACHK49_002157"
     CDS             complement(3465..3719)
                     /gene="yoeB"
                     /locus_tag="ACHK49_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO9262803.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3716..3967)
                     /gene="yefM"
                     /locus_tag="ACHK49_002158"
     CDS             complement(3716..3967)
                     /gene="yefM"
                     /locus_tag="ACHK49_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO9262804.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4250..4297
                     /gene="hisL"
                     /locus_tag="ACHK49_002159"
     CDS             4250..4297
                     /gene="hisL"
                     /locus_tag="ACHK49_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO9262805.1"
                     /translation="MTRVQFKHHHHHHPD"
     misc_feature    4273..4396
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4443..5342
                     /gene="hisG"
                     /locus_tag="ACHK49_002160"
     CDS             4443..5342
                     /gene="hisG"
                     /locus_tag="ACHK49_002160"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO9262806.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5348..6652
                     /gene="hisD"
                     /locus_tag="ACHK49_002161"
     CDS             5348..6652
                     /gene="hisD"
                     /locus_tag="ACHK49_002161"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO9262807.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRQDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6649..7719
                     /gene="hisC"
                     /locus_tag="ACHK49_002162"
     CDS             6649..7719
                     /gene="hisC"
                     /locus_tag="ACHK49_002162"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO9262808.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPAAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7719..8786
                     /gene="hisB"
                     /locus_tag="ACHK49_002163"
     CDS             7719..8786
                     /gene="hisB"
                     /locus_tag="ACHK49_002163"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO9262809.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGDSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8786..9376
                     /gene="hisH"
                     /locus_tag="ACHK49_002164"
     CDS             8786..9376
                     /gene="hisH"
                     /locus_tag="ACHK49_002164"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO9262810.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFALPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9376..10113
                     /gene="hisA"
                     /locus_tag="ACHK49_002165"
     CDS             9376..10113
                     /gene="hisA"
                     /locus_tag="ACHK49_002165"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO9262811.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     LLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVESY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10095..10871
                     /gene="hisF"
                     /locus_tag="ACHK49_002166"
     CDS             10095..10871
                     /gene="hisF"
                     /locus_tag="ACHK49_002166"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO9262812.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCRVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10865..11476
                     /gene="hisIE"
                     /locus_tag="ACHK49_002167"
     CDS             10865..11476
                     /gene="hisIE"
                     /locus_tag="ACHK49_002167"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO9262813.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11573..12550)
                     /gene="wzzB"
                     /locus_tag="ACHK49_002168"
     CDS             complement(11573..12550)
                     /gene="wzzB"
                     /locus_tag="ACHK49_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO9262814.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELGKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12697..13863)
                     /gene="ugd"
                     /locus_tag="ACHK49_002169"
     CDS             complement(12697..13863)
                     /gene="ugd"
                     /locus_tag="ACHK49_002169"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO9262815.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPNTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTKNIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPHVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(14112..15518)
                     /gene="gndA"
                     /locus_tag="ACHK49_002170"
     CDS             complement(14112..15518)
                     /gene="gndA"
                     /locus_tag="ACHK49_002170"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO9262816.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(15629..16036)
                     /locus_tag="ACHK49_002171"
     CDS             complement(15629..16036)
                     /locus_tag="ACHK49_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HFO9262817.1"
                     /translation="MRLVDDNIMDELFRTAANSERLRAHYLLHASHQEKVQRLLIAFV
                     RDSYVEPHWHELPHQWEMFVVMQGQLEVCLYEQNGELQKKFVVGDGTGISVVEFSPGD
                     IHSVKCLSPKALMLEIKEGPFDPLKAKAFSKWL"
     gene            complement(16027..17235)
                     /locus_tag="ACHK49_002172"
     CDS             complement(16027..17235)
                     /locus_tag="ACHK49_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO9262818.1"
                     /translation="MKLALIIDDYLPHSTRVGAKMFHELGLELLSRGHDVTVITPDNT
                     LQAIYSVSMTDGIKVWRFKSGPLKDIGKAKRAINETLLSFRAWHALKHLIQHDTFDGI
                     VYYSPSIFWGGLVKKIKERCQCPSYLVLRDMFPQWVIDAGMIKAGSPIEKYFRYFEKK
                     SYQQADWIGLMSDKNLEIFRQANKGYPCEVLRNWASMTPVSAGDDYHSLRQKYALKDK
                     IIFFYGGNIGHAQDMANLMRLARNMMRHHDAHFLFIGQGDEVDLIKSLSAEWNLTNFT
                     HLPSVNQEEFKLILSEVDVGLFSLSSRHSSHNFPGKLLGYMVQSIPILGSVNGGNDLM
                     DVINKHRAGFIHVNGEDDKLFESAQLLLSDSVLRKQLGQNANVLLKSQFSVESAAHTI
                     EVRLEAGECV"
     gene            complement(17235..18365)
                     /gene="wecB"
                     /locus_tag="ACHK49_002173"
     CDS             complement(17235..18365)
                     /gene="wecB"
                     /locus_tag="ACHK49_002173"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO9262819.1"
                     /translation="MKKLKVMSVVGTRPEIIRLSRVLAKLDEYCDHLIVHTGQNYDYE
                     LNEVFFKDLGVRKPDYFLNAAGKNAAETIGQVIIKVDEVFEQEKPEAMLVLGDTNSCI
                     SAIPAKRRRIPIFHMEAGNRCFDQRVPEETNRKIVDHTADINMTYSDIAREYLLAEGV
                     PADRIIKTGSPMFEVLTHYMSQIDGSDVLSRLNLTPGNFFVVSAHREENVDTPKQLAK
                     LANILNTVAEKYDIPVVVSTHPRTRNRINENGIQFHKNILLLKPLGFHDYNHLQKNAR
                     AVLSDSGTITEESSIMNFPALNIREAHERPEGFEEGAVMMVGLESARVLQALEIIATQ
                     PRGEVRLLRQVSDYSMPNVSDKVVRIIHSYTDYVKRVVWKQY"
     gene            complement(18362..19468)
                     /locus_tag="ACHK49_002174"
     CDS             complement(18362..19468)
                     /locus_tag="ACHK49_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapF"
                     /protein_id="HFO9262820.1"
                     /translation="MKILVTGAAGFIGRNLVFRLKEAGYNELIAIDRNSSLTDLEQGL
                     KQADFIFHLAGVNRPVKESEFEEGNCDLTQQIVDILKKNNKDTPIMLSSSIQAECDNA
                     YGKSKAVAEKIIQQYGETTNAKYYIYRLPNVFGKWCQPNYNSFIATFCHRIANDEAIT
                     INDPSAVVNLVYIDDFCSDILKLLEGANETGYRTFGPIYSVTVGEVAQLIYRFKESRQ
                     TLIIEDVGNGFTRALYSTWLSYLSPKQFAYTVPSYSDDRGVFCEVLKTKNAGQFSFFT
                     AHPGITRGGHYHHSKNEKFIIIRGSACFKFENIVTGERYELNVSSDDFKIVETVPGWT
                     HDITNNGSDELVVMLWANEIFNRSEPDTIARVLS"
     gene            complement(19470..>20449)
                     /locus_tag="ACHK49_002175"
     CDS             complement(19470..>20449)
                     /locus_tag="ACHK49_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFO9262821.1"
                     /translation="VLKRFLDTDITEIRIFSRDEKKQDDMRKKYNNSKLKFYIGDVRD
                     YNSVLNATRGADFLYHAAALKQVPSCEFHPMEAVKTNVLGTENVLEAAIANGIKRVVC
                     LSTDKAVYPINAMGISKAMMEKVIVAKSRNLDSSKTVICGTRYGNVMASRGSVIPLFV
                     DLIKAGKPLTITDPNMTRFMMTLEDAVDLVLYAFEHGNNGDIFVQKAPAATIQTLAIA
                     LKELLNAHEHPINIIGTRHGEKLYEALLSREEMIAAEDMGDYYRVPPDLRDLNYGKYV
                     EHGDRRISEVEDYNSHNTERLDVEGMKELLLKLPFIRALRSGEDYELDS"
BASE COUNT         5434 a         5067 c         4797 g         5151 t
ORIGIN      
        1 ttggttacgc aagccaggta aatcagaccg atagcagccc agatcagacc cagaaccatt
       61 gagctttctt ccaggttaac ccacagcgca ccaacggtca gcgcaccaca catcggcagg
      121 aacagatact ggaagtgatc tttcagcgtc ttgttacgct tctcacggat ccagaactgc
      181 gaaataaccg acaggttaac gaaggtgaac gccaccagcg caccaaagtt aatcagcgcc
      241 gtagccatta ccaggtcgaa gttgattgcc agcaaggcaa tcgcgccaac caggatgatg
      301 ttcattgccg gagtacgcca tttcgggtgt acataaccga agaagctttt cgggaacaca
      361 ccgtcacgcc ccattacgta catcagacgc gctacgcctg catgcgccgc cataccggac
      421 gccagtacgg taatggtgga gaagatcagc gcgccaacct ggaacgcttt acccgcaacg
      481 tacagcatga tttcaggctg tgacgcatcc ggatctttaa agcgagagat atccgggaag
      541 tacagctgca ggaagtaagt tgcaaagatg aagatcatgc cgccaatcag tgcggtcagg
      601 aaaatcgcac gcgggatcac gcgctctgca tctttggttt cttccgacag gttgctgatg
      661 ccgtcaaagc cggtaaagga gaagcacagg attgtcgccc cggtaatcat cgggataaca
      721 tgcgcatcgc cagaccagaa cggacgagtg ctcgccagcg tacccgcgcc ttcaccttca
      781 aatacgccat aaacaaccat gcccagaatc accgcgatca gcactacctg caacacgacg
      841 attacagtgt tgaagttcgc tacggatttc agactacgca ggttaaaggc ggtcataaag
      901 gccaccagcg ccaccacaaa catccacgat gggatggaag gcaccagagc ttcaaaatag
      961 attttcgcca gcagaatgtt gatcatcggc gcgaacagat agtcgagcag agaagaccaa
     1021 cccaccataa agccgacagt cgggctaatg gatttctggg cgtaagtgta tgcagagcca
     1081 gcagaaggat agcggcgaac cagcttcccg tagctcagag ccgtaaacag gatcgcaatc
     1141 aatgcgaacg cataggctgt cggcacatga ccatccgtga ggcctgaaac gataccaaat
     1201 gtatcaaaca gcgtcatcgg ctgcatatag gcaagaccca tcattacaac cggaactaac
     1261 gtaagcgttt tacgcaattc cacgcgagag gtgtttggag taacgttatg cgacatggtc
     1321 attctccttt acggtgataa ccgtcgcgta agcaaaaaat tgccccattt ttttggattc
     1381 ctcagcgaca acaactgtcg atttttagta aatatctatc cggtacgaag cccggcctct
     1441 tggtatgaat gattggtttg aagcaaaaaa taaccgacgc taattaaacg tcggttttta
     1501 gtcatttttt gacagcggcg cattgtgcct aaatgggggg gaaatgacaa gagaatgaga
     1561 ggcttgtcat aataattttt ctttaaatgg ctgattttcc gtcatcagat tttgcataaa
     1621 caccggcgat cgcactattt gctaaaattt cgtcccgcca ccaaccactg gcaagccccg
     1681 cggtctgttc attccagcca atccagacgg gttcaaagag ggtttgggcg accactttct
     1741 tctcgattaa cgcaccacta tcgagaaaac gttgcgccag ataacggggt aaatagccgc
     1801 agccgaggcc gctaatttgc agctccagct tggttttaaa atcgaaaacg gtaatagctt
     1861 cttgttcgtc gagaagttgt gaggccgtag aagcgccggc ctgtgcagtg tcccccacca
     1921 caatcgcccg atatcgttta atgatacgac ggtttaacgg ttcttcttcc agggctaacg
     1981 gatgatgagg cgcgaccgca aagacttgtt ccagatcgcc caaccgagag aaaccaaact
     2041 cactggaaga tggtggctca tgcatcgcgc caacaataat atccgctcgc ccctgagtca
     2101 gggcatccca tgaaccaccg agtacaccat tgataaactt caggcgcgtc acgctatgat
     2161 gttgataaaa agcctcaatg agtggcgcaa gcagagaaaa agggaaggtg tcgtccacgc
     2221 caatcaccag ctcgttttcc cagccttcat gcagtttgat cgcctgcttt tccagttctc
     2281 gcacggtatg cagaacttcc cgccctttct ctaataacat ttttccggtg cgggtgaatt
     2341 tagcgcggtg tccgctgcga tcaagcaatt gaatattaag gtcgctttcc agcttgtgaa
     2401 cggtgtaact cagagcggac ggcgtcttgt agagtttggc cgacgccgcc gcaaaactgc
     2461 cttctttttc taaggcatcg aggatcatca acacatccag cagtggtttc atacacgccc
     2521 ccttgcagtg tatgcggcgt cctgctggcg tgatcgttta ctccagcggc attaccagcg
     2581 gatcgggata ctggtattca aatcccagtt cattacaaat ccgactgcca tcaataatct
     2641 tacctttgcc gctgtccaga ctatttctga actgcggagg ttccagcccc aataaacggg
     2701 ccatctgcgg atagaaaaca ttacgcgcag ggtgagctgg cgcacatata ttatagatgt
     2761 gtccgccttt aggtgcctgt aacaacagag tgatagcgcc aataacatct tctaaatgga
     2821 ctaaattaac accatgttca ccatcagggg cggtttttcc ggcaaagaag cgtccgggat
     2881 gacgtcccgg tccgaccagg cccgcaagac gcagaatatc gaccgaagta ccgggtaaat
     2941 tgtgcagcca gtcttcgagt tcttctaata ctcgtccact gttggtcacc ggattacgcg
     3001 gggtggtttc tttcaccgtg ccttgcgcgt cgccatagac agatgtcgag ctggtaaaaa
     3061 taatgcgggg aatacgatgg gccagcgcgc tatccactaa ctcttgtacc gcttgtaaat
     3121 agaactcatc gccggggccg ctacgacgtg ccggaagcgt aatgaccagc gcatcggcat
     3181 ccatcagggc atccagatcg tcagaatcgc aaactaactc aggctccatg cgaagcagat
     3241 agctatcaat gccactcatt cgggccgctt cgacaccatc ttgtgtggtt ttactcccgg
     3301 tgacttgcca gcctcgcgct gaaagtgaca tcgccagcgg catgcctaac caccctaacc
     3361 cgacaattgc gacctttttc atccgttatc tcctgataca ccttagatct ataaggctac
     3421 gctagcgtat caaaactgac aattcattct atgaatgaat ctgttcaata atgataacga
     3481 catgctgcaa tgagcagtga atcatcagta accgcgtata ccagacggtg ctcctctgta
     3541 atgcgtcggg accagaaacc tgacaaatta tgtttcaggg gttctggttt ccccttacct
     3601 tcaaatggtg ttctgcgggt atctttgata agttcattga tctttttaac aattcgctta
     3661 tctgtttcct gccagtacag ataatcatcc catgattcct cagaccagat tagtttcact
     3721 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
     3781 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgag ttgtattctt
     3841 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
     3901 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
     3961 tacgcatgta aatctcctct tttgtacagt tcattgtaca atgatgggtg ttaattcact
     4021 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
     4081 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttt cccgctcatt
     4141 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
     4201 tggtttaggt taaaagacat cagttgaata aacattcaca gagactttta tgacacgcgt
     4261 tcaatttaaa caccatcatc accatcatcc tgactagtct ttcaggcgat gtgtgctgga
     4321 agacattcag atcttccagt ggtgcatgaa cgcatgagaa agcccccgga agatcacctt
     4381 ccgggggctt ttttattgcg cggttgttaa cggttcagac aggtttaaag aggaataaga
     4441 aaatgacaga caacactcgt ttacgcatag ctatgcagaa atccggccgt ttaagtgatg
     4501 actcacgcga attactggcg cgctgtggca ttaaaatcaa tcttcacacc cagcgcctga
     4561 tcgcgatggc agaaaacatg ccgattgata ttctgcgcgt gcgtgacgat gacattcccg
     4621 gtctggtaat ggacggcgtg gtagaccttg ggattatcgg cgaaaacgtg ctggaagaag
     4681 agctgcttaa ccgccgcgcc cagggtgaag atccgcgcta ctttaccttg cgtcgtctgg
     4741 atttcggcgg ctgccgcctt tcgctggcaa cgccggttga tgaagcctgg gacggtccgc
     4801 tctccttaaa tggtaaacgt atcgccacct cttatcctca cctgctcaag cgttatctcg
     4861 accagaaagg tatctctttt aaatcctgct tactgaacgg ctctgtcgaa gttgcgccgc
     4921 gtgccggact ggcggatgcg atttgcgatc tggtttccac cggtgccacg ctggaagcta
     4981 acggcctgcg cgaagtcgaa gttatctacc gctcgaaagc ctgcctgatc cagcgcgatg
     5041 gcgaaatgga agaatccaaa cagcaactga tcgacaaact gctgacccgt attcagggtg
     5101 tgatccaggc gcgcgaatca aaatacatca tgatgcacgc accgaccgaa cgtctggatg
     5161 aagtcatcgc cctgctgcca ggtgccgaac gcccaaccat tctgccgctg gcgggtgacc
     5221 aacaacgcgt agcgatgcac atggtcagta gcgaaaccct gttctgggaa accatggaaa
     5281 aactgaaagc gctgggtgcc agttcaatcc tggtcctgcc gattgagaag atgatggagt
     5341 gatcgccatg agctttaaca caatcattga ctggaatagc tgtactgcgg agcaacaacg
     5401 ccagctgtta atgcgcccgg cgatctccgc ttctgaaagc attacccgca ccgttaacga
     5461 tattctcgat aacgtgaaag cacgcggcga tgacgccctg cgggaataca gcgcgaagtt
     5521 tgataaaacc acggttaccg cgctgaaggt gtctgctgag gagatcgccg ccgccagcga
     5581 acgccttagc gacgagctaa aacaggcgat ggcggtggca gtaaaaaata ttgaaacctt
     5641 ccacactgcg caaaaactgc cgccggtaga tgttgaaaca cagccaggcg tgcgttgcca
     5701 gcaagtcacg cgcccggtag cttcagttgg gttgtatatt cctggcggct ccgccccact
     5761 cttctcaacg gtattaatgc tggcaactcc tgcgcgtatt gcgggctgta aaaaagtggt
     5821 gttgtgctca ccgccgccga ttgccgatga gatcctttat gcggcgcagc tgtgcggtgt
     5881 gcaggacgtg tttaacgtcg gcggcgcaca ggccattgcc gctctggcgt ttggtacgga
     5941 atctgtgccg aaagtggaca aaatcttcgg gccgggtaac gcctttgtca ccgaagcgaa
     6001 acgtcaggtg agccagcgtc aggacggtgc ggcgatcgat atgcccgcag gcccgtcgga
     6061 agtgctggtg attgctgaca gcggcgctac gccagatttc gtggcttctg atttgctttc
     6121 tcaggctgaa cacggcccgg actcacaggt gattttactg acgcccgctg ctgatatggc
     6181 gcgtcgcgtt gccgaggccg tcgaacgcca gttggcagaa ctgccgcgtg ccgaaaccgc
     6241 ccgccaggca ctgaacgcca gccgcctaat cgtgaccaac gatttagcac agtgcgtgga
     6301 gatctccaac cagtacggcc cggagcacct gatcattcag acccgcaacg ctcgcgatct
     6361 ggtcgatagc atcaccagcg ccggttcggt atttcttggt gactggtcac cggaatcggc
     6421 aggtgattac gcctccggca ccaaccacgt tctgccgact tacggttaca ccgccacctg
     6481 ttccagcctc gggctggcag atttccagaa gcgcatgacc gtgcaggaac tgtcgaaaga
     6541 aggcttttct gcgctggctt caaccataga aacactggcc gccgccgagc gcctgaccgc
     6601 ccataaaaat gccgttactt tgcgtgttaa cgcccttaag gagcaagcat gagcaccgtg
     6661 actattaccg atttagcgcg tgaaaacgtc cgcaacctga cgccgtatca gtcggcgcgt
     6721 cgtctgggcg gtaacggcga cgtctggctg aacgccaacg aatatcccgc agccgtggag
     6781 tttcagctta ctcaacaaac gctcaaccgc tacccggaat gccagccgaa agcggtgatt
     6841 gaaaattacg cgcaatatgc aggcgtaaaa ccggagcagg tgctggtcag ccgtggcgcg
     6901 gacgaaggta ttgaactgct gattcgcgct ttttgcgaac cgggtaaaga cgccatcctc
     6961 tactgcccgc caacgtacgg catgtacagc gtcagcgccg aaacgattgg cgtcgagtgt
     7021 cgcacagtgc cgacgcagga caactggcaa ctggacttac agggcatttc cgacaagctg
     7081 gacggcgtaa aagtggtcta tgtttgcagc cccaacaacc cgaccgggca actgatcaat
     7141 ccgcaggatt ttcgcactct gctggagcta acccgcggaa aagccattgt ggttgccgat
     7201 gaagcctata tagagttttg tccacaggca tcgctggctg gctggctggc ggaatatccg
     7261 cacctggcta ttttgcgcac actatcgaaa gcttttgctc tggcggggct tcgttgcgga
     7321 tttacgctgg caaacgaaga agtcatcaac ctgctgatga aagtgatcgc cccctacccg
     7381 ctctcgacgc cggttgccga cattgcggcc caggcgttaa gcccgcaggg gatcgtcgcc
     7441 atgcgcgaac gggtggcgca aattattgca gaacgcgaat acctgattgc cgcactgaaa
     7501 gagatcccct gcgtggagca ggtttttgac tctgaaacca actacattct ggcgcgcttt
     7561 aaagcctcca gtgcagtgtt taaatctttg tgggatcagg gcattatctt acgtgatcag
     7621 aataaacaac cctctttaag cggctgcctg cgaattaccg tcggaacccg tgaagaaagc
     7681 cagcgcgtca ttgacgcctt acgtgcggag caagtttaat gagtcagaag tatcttttta
     7741 tcgatcgcga tggaaccctg attagcgaac cgccgagtga ttttcaggtg gaccgttttg
     7801 acaaactcgc ctttgaaccg ggcgtgatcc cggagctgct gaagttgcaa aaagcgggct
     7861 acaaactggt gatgatcact aatcaggatg gtctgggaac acaaagtttc ccgcaggcgg
     7921 atttcgatgg cccgcacaac ctgatgatgc agatcttcac ctcgcaaggc gtacagtttg
     7981 atgaagtgct gatttgtccg cacctgcccg ccgatgagtg cgactgccgt aagccgaaag
     8041 taaaactggt ggagcgttat ctcgctgagc aagcgatgga tcgtgccaat agttatgtga
     8101 ttggcgatcg cgcgaccgac attcaactgg cggaaaacat gggtattaat ggtttacgct
     8161 acgaccgcga aaccctgaac tggccaatga ttggcgagca actcactaaa cgagaccgtt
     8221 acgcccatgt agtgcgtaac accaaagaga cgcaaattga cgtccaggtg tggctggatc
     8281 gcgaaggtga cagcaagatt aacaccggcg ttggcttctt tgatcacatg ctggatcaga
     8341 tcgccaccca cggcggtttc cgtatggaaa tcaacgtcaa aggcgacctc tatatcgacg
     8401 atcaccacac agtcgaagat accggcctgg cgctgggtga agcgttaaaa attgcccttg
     8461 gcgacaaacg cggtatttgc cgctttggtt ttgtgctacc gatggacgaa tgccttgccc
     8521 gctgcgcgct ggatatctct ggtcgcccgc acctggaata taaagccgag tttacctacc
     8581 agcgcgtggg cgatctcagc accgaaatga tcgagcactt cttccgttcg ctctcttaca
     8641 ccatgggcgt gacgctacac ctgaaaacca aaggtaaaaa cgatcatcac cgtgtagaga
     8701 gcctgttcaa agcctttggt cgcaccctgc gccaggccat ccgcgtggaa ggcgacaccc
     8761 tgccctcgtc aaaaggagtg ctgtaatgaa cgtggtgatc cttgataccg gctgcgccaa
     8821 tctgaactcg gtgaagtctg ccattgcgcg ccacggttat gaacccaaag tcagccgtga
     8881 cccggacgtc gtgttgctgg ccgataaact gtttttaccc ggcgtaggca ccgcacaagc
     8941 agcgatggat caggtgcgtg agcgcgaact gtttgatctc atcaaagcct gtacccaacc
     9001 ggtgctggga atctgcttag ggatgcaact gctggggcgg cgcagcgaag agagcaacgg
     9061 cgtcgacttg ctgggcatca ttgacgaaga cgtgccgaaa atgaccgact ttgctctgcc
     9121 actgccacat atgggctgga accgcgttta tccgcaggca ggcaaccgcc tgtttcaggg
     9181 aattgaagac ggcgcgtact tttactttgt tcacagttac gcaatgccgg tcaatccgtg
     9241 gaccatcgcc cagtgtaatt acggcgaacc gttcaccgcg gcggtacaaa aagataactt
     9301 ctacggcgtg cagttccacc cggagcgttc tggtgccgct ggcgctaagt tgctgaaaaa
     9361 cttcctggag atgtgatgat tattccggca ttagatttaa tcgacggcac tgtggtgcgt
     9421 ctccatcagg gcgattacgg caaacagcgc gattatggta acgacccgct gccgcgattg
     9481 caggattacg ccgcgcaggg tgcagaattg ttgcatctgg tggatctgac cggggcaaaa
     9541 gatccggcta aacgtcaaat cccgctgatt aaaaccctgg tggcgggcgt taacgtgccg
     9601 gtgcaggttg gcggtggcgt gcgtagcgaa gaagacgtgg cggcgttact ggaagcaggc
     9661 gttgcgcgcg tggtggttgg ctccaccgcg gtgaaatcac cagagatggt gaaaggctgg
     9721 tttgaacgtt tcggtgccga tgccttagtg ctggcgctgg atgtccgtat tgacgagcaa
     9781 ggcaacaagc aggtggcagt cagcggctgg caagagaact cgggcgtttc actggaacaa
     9841 ctggtggaaa gctatctgcc cgtcggcctg aaacatgtgc tgtgtaccga tatctcgcgc
     9901 gacggcacgc tggcaggttc taacgtctct ttatatgaag aagtgtgcgc cagatatccg
     9961 caggtggcgt ttcagtcctc cggcggtatt ggcgacattg atgatgtggc ggccctgcgt
    10021 ggtactggcg tgcgcggcgt aatagttggt cgtgcattac tggaaggtaa attcaccgtg
    10081 aaggaggcca tcgcatgctg gcaaaacgca taataccgtg tctcgacgtc cgtgatggtc
    10141 aggtggtgaa aggcgtacag tttcgcaacc atgaaatcat tggcgatatc gtgccgctgg
    10201 caaaacgcta cgctgaagaa ggtgcagacg aactggtgtt ctacgatatc accgcttcca
    10261 gcgatggccg tgtggtagat aaaagctggg tatctcgcgt ggcggaagtg atcgacattc
    10321 cgttctgcgt ggcgggtggg attaagtctc tggaagatgc cgcgaaaatt ctttcctttg
    10381 gcgcggataa aatttccatc aactcccctg cgctggcaga cccaacatta attactcgcc
    10441 tggcggatcg ttttggcgtg cagtgtattg tggtgggtat tgatacctgg tacgacgctg
    10501 aaaccggtaa atatcatgtg aatcaatata ccggcgatga aagccgcacc cgcgtcactc
    10561 agtgggaaac actcgactgg gtacaggaag tgcaaaaacg cggtgccgga gaaatcgtcc
    10621 tcaacatgat gaatcaggac ggcgtgcgta acggttacga cctcgagcag ctgaaaaaag
    10681 tgcgagaagt ttgccgcgtc ccgctgattg cctccggtgg cgcgggcacc atggaacact
    10741 tcctcgaagc cttccgtgat gccgacgttg acggcgcgct ggcggcttcc gtattccaca
    10801 aacaaataat caatattggt gaattaaaag cgtacctggc aacacagggc gtggagatca
    10861 ggatatgtta acagaacaac aacgtcgcga actggactgg gaaaaaaccg acggactgat
    10921 gccggtgatt gtgcaacacg cggtatctgg cgaagtgcta atgctgggct atatgaaccc
    10981 ggaagcctta gacaaaacca tcgaaagcgg caaagtcact ttcttctcgc gcactaaaca
    11041 gcgactgtgg accaaaggcg aaacgtcggg caatttcctc aacgtagtaa atattgcgcc
    11101 ggactgcgac aacgacacgc tactggtact ggcgaatccc atcgggccaa cctgccacaa
    11161 aggcaccagc agctgcttcg gcgacaccgc tcaccagtgg ctgttcctgt atcaactgga
    11221 acaattgctc gccgaacgca aatctgccga tccggaaacc tcttacacag ccaaactgta
    11281 tgccagcggc accaaacgca ttgcacagaa agtgggcgaa gagggcgtgg aaaccgcact
    11341 agcagcaacg gtacatgacc gctttgaact caccaacgag gcgtctgact tgatgtatca
    11401 cctgttggtg ttgttgcagg atcaggatct ggatttaaca acggtgattg agaacctgcg
    11461 taaacggcat cagtgagttg cggggtaagc ggatgcgatg ttgttgtcgc atccggcaaa
    11521 aaaaacgggc aaggtgtcac caccctgccc gttttcttta aaaccaaaaa gattacttcg
    11581 cgttgtaatt acgcagagcg ttacgcccca gcacaatccc cgcgccaacc atgccgccca
    11641 gcagcaccgc cagaatcaaa gtgattgcct ttttcgggct atcgcgacga ataggtaacg
    11701 tcggtttcat cacatagcgg taagcatgaa tatcaagatc atcaactttt aagctttcga
    11761 tatccagcag gttttgacgt gtctgatagt agtttggtga gaacaccaac ggacgagtcg
    11821 cttcatgctt aatcatcgac tccagcgctt cgctccccaa aaggaacaac gtatcttgtg
    11881 tcacatcttc agtctgctga acctgtggct ttgtcacctg cgcctgattc gcatactgca
    11941 acgcttcctg aatctgacgg atacgcagat ctttctgctc ctgcgcaact acttcctgag
    12001 ttttcagaga atcctgcaag tttttcatac gcagcgcgat gttgtctttc aggtcttttc
    12061 ccagttcttc atttacctgg tcgtcaactt gctggatata cttcgccagc tgcttctgag
    12121 caccttcagg tgtttcacca acataagaca ccgctaaagg taatgactga tttttgacgg
    12181 taggctcaat ggtcagcttt tctgcttctt cctggttatc cagcgtttcc gctaatgctg
    12241 agaatgcagt gctgtaacgt ccgataagtg aagcctgaat gtcggagact ttcggtacag
    12301 ccgatccgta gatcacattc atcgcgttgg tataaccagc gatttgcccc acatcaggtt
    12361 gagtaacgat agctgtggaa gtccactttt ctttggcaac ggccagatac cctatagcca
    12421 gggcaatagc aacaatgaca gagatgataa ttgtcatctt gccacgccac aactgcacca
    12481 gtaaatcaat caaatcaatc tgttccgggt catggttttg cccagaaaca ttattatttt
    12541 ctactctcat cattacccta acggacgaaa taattttaag tcgccatagt ttagcgataa
    12601 tcgccatgaa tgctatagga taaatgataa aaataatgaa ttattacaaa gggaacataa
    12661 gagaatcagg gcgaaaatcg ccctgataac aggatgttag tcactgccaa agagatcgcg
    12721 ggtgtagact ttgtctgcca catccttaag ctcttctgcc atacggttgg agataattac
    12781 gtcggcttgt tgtttgaaag tggcgagatc acgttccagc cgagagttga agaatgagtc
    12841 ttctttcatc actggctcgt agatgattac ttcaacacct ttcgccttga tacgcttcat
    12901 aatcccctga atagaagacg cacggaagtt atctgaaccg ctcttcataa tcaggcgata
    12961 aatacccacc acatgcggtt tgcgtgacaa aatggcatcg gcaataaaat ctttacgcgt
    13021 gcggtttgca tcgacaattg ccgagatcag gttattcggc acagactgat agttcgccag
    13081 taactgcttg gtatctttcg gcagacaata accaccataa ccaaacgacg gattgttgta
    13141 atggttgcca atacgcggat cgagacaaac gccttcgata atctggcgag tattcagacc
    13201 taaactttct gcgtagctat ccagttcatt aaagtacgct acgcgcatcg ccagataggt
    13261 attagcgaaa agtttaatcg cttctgcttc ggttgagtcg gtaaacaggg tcgggatatt
    13321 ttgcttaatc gcgccttcct gtaacagagc agcaaaacgt tctgcgcgtt ctgaacgctc
    13381 accgatgaca atacgtgaag gatggagatt atcgtaaagg gctttaccct cacggagaaa
    13441 ttccggagag aaaataatat ttttagtacg atatttctta tgcatcgctg cggtaaagcc
    13501 aacgggtacc gttgatttga tgaccataac cgcataagga tttatctcta ctacgtcctt
    13561 aattactgat tctacactcg atgtattgaa ataattagtg ttaggatcat agtcggttgg
    13621 agtggcgatg atgacataat cagcatcccg gtaggcttca tttttatcta atgtggcatt
    13681 aaagtgtatt ttatctgatt gcaaaaactg ctgaatttcc ttatcaacaa taggagatat
    13741 ccgatcattc agcatagcaa cgcgtgacgg taaaatatct aatgccacaa cctcatgatt
    13801 ttgtgcgatt agaagcccgt ttgacaagcc tacatagccg gtaccggaaa tggtgatttt
    13861 catttcatgc tctcagaatt aacttaactg tgaatcatga tgtttttagc atcctgataa
    13921 gagctaaaag ttttaacgct acgttgttcg atggtcaacg caacgaaaaa caattcagat
    13981 tttctttata agaatagctg atatttatta agttaatatt aagcagtgaa atttagtcta
    14041 taagataatt ggcaaaaaaa aagcccggcg taatatacgc cgggcctcaa tttattgttg
    14101 gttaaatcag attaatccag ccattcagta tggaacacac cttctttatc aatgcgctta
    14161 taagtatgcg caccaaaata gtcacgctga gcctggatca ggttcgcagg cagaacagcg
    14221 gcacggtagc tgtcataata ggcaaccgca gcggcgaagg tcggaaccgg gataccgttc
    14281 tgtactgcat aagcaacgac atcacgcagc gcctgctggt agtcatcggc aatttgcttg
    14341 aagtacggag ccaacagcag gttagcgatc tgaggatttt cggcataagc atcagttatt
    14401 ttctgcagga actgcgcacg gatgatgcag ccagcacgga aaatcttcgc gatttcgccg
    14461 tagttcagat cccagttgta ctcttcagac gctgcgcgca gctgagagaa gccctgagcg
    14521 taagaaacga ttttgcccag atacagcgca cggcgaactt tttcgatgaa ctcagccttg
    14581 tcgccagctg gctgcgcttg cgggccagag agaactttag atgcggcaac acgctgatct
    14641 ttcagagaag agatataacg tgcaaacaca gactcggtaa tcagcgacag cggttcaccg
    14701 agatccagcg cgctctggct ggtccattta ccggtacctt tgttagccgc ttcatccagg
    14761 atcacatcaa ccaggtagtt accgtcttca tcttttttgg tgaagatgtc tttggtgatg
    14821 tcgatcaggt agctactcag ttcaccgtta ttccactcgg taaaggtctg tgccagttct
    14881 tcgttagaga gattcaggcc gcctttcagg agagaatagg cttcagcaat cagttgcata
    14941 tcaccgtatt caataccgtt gtgaaccatc ttcacatagt gacccgcgcc atcagcacca
    15001 atataggtaa cgcacggttc gccatcttca gcaacggcgg cgattttagt caggatcggt
    15061 gcaaccagtt cataggcttc tttctgccca ccaggcataa tggaaggacc tttcagtgcg
    15121 ccttcttcac caccggagac accggtacca atgaagttaa aaccttctgc agaaagctca
    15181 cgattacgac gaatggtgtc ctggaagaag gtattaccac catcaatgat gatgtcacct
    15241 ttatcgaggt atggcttaag ggaatcaata gcagcatccg tgcctgcacc tgctttcacc
    15301 attaacagga tgcgacgagg cgtttccaga gattcaacaa actctttcac cgtatagtaa
    15361 ggaaccagtt tcttgcctgg attttcggca atcacttctt ccgtcttttc acgggaacgg
    15421 ttgaaaatag agacggtata accacggctt tcgatgttga gcgcaaggtt gcgccccatc
    15481 actgccatac cgactacgcc gatctgttgc tttgacattg tttactcctg tcaggggtgg
    15541 tcacgcgggg ttacgcggct tgaagaagct aagatggtaa cccagcatgt atagaataag
    15601 atagaagaat aaacggtggt gtatcgccct ataaccactt agaaaaagcc ttagctttca
    15661 gtgggtcaaa tggcccctcc tttatctcta acataagggc ttttggtgac aggcatttga
    15721 cactatgtat atctcctggg gaaaattcca cgacacttat tcccgtaccg tctccaacaa
    15781 caaacttttt ttggagctca ccattttgct catacaaaca aacttctaat tgcccttgca
    15841 tgacgacaaa catttcccac tgatgcggta actcatgcca atggggttca acatagctgt
    15901 cgcgtacaaa tgcaataagt aaacgttgaa ccttctcctg atgagatgcg tgcaataaat
    15961 aatgagcgcg caaacgttca gaatttgctg cagtgcgaaa aagttcatcc ataatattgt
    16021 catcaactaa acgcattctc ctgcctccag tcggacttcg atagtatgtg ccgccgattc
    16081 aaccgaaaat tgagacttta acaacacatt ggcgttctga cctaactgtt ttcttaaaac
    16141 tgaatcacta agaagcaatt gtgcagattc aaacagttta tcatcttcac cattaacatg
    16201 aatgaaccca gccctgtgct tattaattac atccattaga tcattaccgc cattcacact
    16261 cccaaggatc gggattgatt gaaccatata ccctagtaat tttccgggga aattatgtga
    16321 agaatggcga gacgaaaggg agaacaggcc aacatcaact tcagataaaa ttaatttaaa
    16381 ctcttcctgg ttcactgaag gtagatgagt gaaattagtt aaattccatt ctgcagaaag
    16441 agatttaatc aggtcaactt catcaccctg ccctataaac aggaaatgag catcatgatg
    16501 acgcatcata ttacgcgcaa ggcgcattaa gtttgccata tcctgagcat gcccaatatt
    16561 accgccatag aaaaaaatga ttttatcttt tagagcgtat ttttgacgaa gtgaatgata
    16621 atcatcgccg gcagacacag gagtcattga ggcccaatta cgtaaaactt cacacggata
    16681 acctttattg gcctgacgaa atatctcaag attcttatca gacattaacc caatccagtc
    16741 agcctgttga tatgattttt tttcaaaata cctgaaatat ttttcaattg gcgagccggc
    16801 ttttatcata ccagcatcaa tgacccactg tgggaacata tcccttagga ccagatagct
    16861 tgggcactgg catcgctctt taattttttt aaccaagcct ccccaaaaaa tagaggggga
    16921 ataataaacg ataccatcaa atgtatcatg ttggatgaga tgctttaatg cgtgccatgc
    16981 acgaaaagat aaaagagttt catttatggc acgtttagcc ttacctatat cctttaaagg
    17041 tccacttttg aaacgccaaa cctttatacc atcagtcatg ctaacagaat agattgcctg
    17101 taatgtgttg tcaggcgtaa ttacagttac atcatggcct ctgctcagca attccaggcc
    17161 taactcatga aacattttag ccccaactcg tgtgctatgg ggcaaataat catcaatgat
    17221 taatgcaagt ttcattagta ttgcttccag acaacccgtt taacgtagtc agtatatgaa
    17281 tggataatac gcacaacttt atctgaaaca tttggcatac tatagtcact gacctgacga
    17341 agtaagcgta cttctccacg aggctgtgtt gcaataattt ctaatgcctg taacacacgg
    17401 gcagactcaa gaccaaccat cattacagcc ccttcttcga agccttcagg gcgttcgtgc
    17461 gcttctcgta tattgagcgc agggaagttc ataatggagg actcctctgt aatagtcccg
    17521 ctatccgata aaacagcacg tgcatttttt tgcagatggt tgtaatcgtg aaatcctaat
    17581 ggcttaagta gcaagatatt tttatgaaat tgaataccgt tttcgttgat gcggttacga
    17641 gtgcgcggat gagtagaaac aactaccggg atatcatatt tttcagccac ggtattaagt
    17701 atatttgcca gtttcgcaag ctgtttaggg gtatcaacat tttcttctct gtgggcactt
    17761 accacaaaga aattcccagg tgttaaattc aggcgagaaa gtacatcgga accatcaatc
    17821 tgcgacatat aatgagtgag tacttcaaac attgggctac cggttttaat aatcctatcg
    17881 gctggtacac cttcagccaa aagatattca cgcgcgatat cactatatgt catattgata
    17941 tcagcggtgt ggtcaactat ttttctgtta gtttcttccg gtacgcgttg atcaaaacaa
    18001 cgattcccag cctccatatg gaaaatcgga attcttcgac gctttgctgg tattgctgaa
    18061 atacaggagt tagtatcgcc aagaactaac atggcttctg gtttttcctg ttcaaagacc
    18121 tcatcaactt taatgataac ttgtccaata gtctctgctg catttttacc tgcggcatta
    18181 agaaaataat caggtttgcg aacacccaaa tccttgaaaa aaacctcatt caattcataa
    18241 tcgtaatttt gcccagtatg aacaataagg tgatcacaat attcatctaa ttttgcaagg
    18301 acacgcgaga gtcgaataat ttctggacga gtcccaacaa ccgacatgac tttcaatttt
    18361 ttcacgataa aactctcgct atagtatctg gttcagaacg attaaatatt tcatttgccc
    18421 aaagcataac aactagctca tccgagccat tattagtaat gtcatgcgtc catcccggaa
    18481 ctgtttcaac aattttaaaa tcatctgatg aaacattaag ttcatatcgt tcacccgtga
    18541 caatattttc aaatttgaaa caagcacttc ctcggatgat aataaatttc tcatttttgg
    18601 aatgatgata atgccccccc cgagtaattc ctggatgcgc agtaaaaaac gaaaactggc
    18661 ccgcgttttt cgttttcaat acttcacaga atacccctct gtcatcacta taagaaggaa
    18721 ccgtatacgc aaactgttta ggagaaaggt aacttaacca tgttgagtac aatgctcgag
    18781 tgaatccatt acctacatct tcgatgatta atgtttggcg actttcttta aatcgataaa
    18841 ttaattgtgc cacctcacca acagtaacag aataaattgg accaaatgtc ctgtaaccag
    18901 tttcgttcgc tccttctaat agctttaata tgtcagaaca aaagtcatct atatacacca
    18961 aatttacaac tgctgaagga tcattaattg taatagcttc atcatttgca atgcgatggc
    19021 agaaagttgc tataaaggag ttataatttg gttgacacca cttaccgaat acattcggca
    19081 agcgataaat ataatattta gcgtttgtcg tttccccata ctgctgaatg attttttccg
    19141 caactgcttt actctttcca taagcgttat cacattcagc ttggatggat gaactaagca
    19201 tgataggagt atctttatta ttttttttca gaatatcaac aatctgttga gttagatcgc
    19261 aattcccctc ttcaaactca ctctccttca caggacgatt tactccagca agatgaaaaa
    19321 tgaaatctgc ctgcttaagt ccctgctcta aatccgtcaa agaagagtta cgatctatcg
    19381 caataagttc gttatatcca gcttccttaa ggcggaaaac caaatttcga ccgataaacc
    19441 ctgcagcgcc agtaactaaa attttcatat tatgaatcca actcataatc ttcaccagaa
    19501 cgaagtgccc ggataaaagg aagttttagc agtaattctt tcattccctc aacatctaat
    19561 ctctcagtat tatgagaatt ataatcttcc acttccgaga tacgacggtc accatgttcc
    19621 acatattttc catagttcaa atcgcggaga tctggtggaa cacgataata atcacccata
    19681 tcttccgctg caatcatttc ctctcggctc aataacgctt cgtaaagttt ttccccgtgt
    19741 cgagttccaa taatattgat tggatgctca tgggcattta gcaattcctt aagtgcaatg
    19801 gctaatgttt gaattgttgc cgcaggtgct ttctgaacga aaatgtcacc attatttcca
    19861 tgttcgaaag cataaaggac cagatcgaca gcatcctcaa gcgtcatcat gaaacgagtc
    19921 atattgggat cggttatggt caatggttta ccagctttga ttagatcaac aaataatggg
    19981 atgaccgatc cacgtgaagc cattacattt ccataacgag ttccacagat aactgttttt
    20041 gaactgtcaa gattacgtga ttttgcaaca ataacttttt ccatcattgc cttagatatg
    20101 cccattgcat tgataggata aacggcttta tcggtactca agcacaccac gcgtttaatc
    20161 ccattcgcaa tagcagcctc cagaacattt tccgtaccca gaacatttgt cttaaccgcc
    20221 tccatagggt gaaattcaca tgaaggaact tgtttaaggg ctgctgcatg atacagaaaa
    20281 tcggcaccac gcgttgcatt tagaacggaa ttatagtctc gcacatcacc tatataaaat
    20341 tttaattttg agttattata ttttttccgc atatcatctt gttttttttc atccctgcta
    20401 aatattcgta tttcagtaat atctgtatct agaaaacgct taagtacgg
//
LOCUS       DAZGLJ010000067        20319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000067 DAZGLJ010000000
VERSION     DAZGLJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.067"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            152..247
                     /locus_tag="ACHK49_002176"
                     /pseudo
     CDS             152..247
                     /locus_tag="ACHK49_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            271..4785
                     /locus_tag="ACHK49_002177"
     CDS             271..4785
                     /locus_tag="ACHK49_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO9262822.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKMAQQSNIPVTVERQLYRLIPGEEEMSFILQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            4786..6435
                     /gene="yfaQ"
                     /locus_tag="ACHK49_002178"
     CDS             4786..6435
                     /gene="yfaQ"
                     /locus_tag="ACHK49_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO9262823.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            6440..7216
                     /gene="yfaP"
                     /locus_tag="ACHK49_002179"
     CDS             6440..7216
                     /gene="yfaP"
                     /locus_tag="ACHK49_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO9262824.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            7491..10340
                     /gene="rcsC"
                     /locus_tag="ACHK49_002180"
     CDS             7491..10340
                     /gene="rcsC"
                     /locus_tag="ACHK49_002180"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO9262825.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(10426..11076)
                     /gene="rcsB"
                     /locus_tag="ACHK49_002181"
     CDS             complement(10426..11076)
                     /gene="rcsB"
                     /locus_tag="ACHK49_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO9262826.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(11093..13765)
                     /gene="rcsD"
                     /locus_tag="ACHK49_002182"
     CDS             complement(11093..13765)
                     /gene="rcsD"
                     /locus_tag="ACHK49_002182"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO9262827.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            14504..15595
                     /gene="ompC"
                     /locus_tag="ACHK49_002183"
     CDS             14504..15595
                     /gene="ompC"
                     /locus_tag="ACHK49_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO9262828.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGRGYDDEDILKYVDVGATYYFNKNMSTYVDYK
                     INLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            15707..16762
                     /gene="apbE"
                     /locus_tag="ACHK49_002184"
     CDS             15707..16762
                     /gene="apbE"
                     /locus_tag="ACHK49_002184"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO9262829.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQTAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            16836..17900
                     /gene="ada"
                     /locus_tag="ACHK49_002185"
     CDS             16836..17900
                     /gene="ada"
                     /locus_tag="ACHK49_002185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO9262830.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADSEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANACEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            17900..18550
                     /gene="alkB"
                     /locus_tag="ACHK49_002186"
     CDS             17900..18550
                     /gene="alkB"
                     /locus_tag="ACHK49_002186"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO9262831.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            18626..20269
                     /gene="yojI"
                     /locus_tag="ACHK49_002187"
     CDS             18626..20269
                     /gene="yojI"
                     /locus_tag="ACHK49_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO9262832.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTNVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDRLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGQTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         4895 a         4998 c         5453 g         4973 t
ORIGIN      
        1 ccttgcgcta atgctctgtc tcaggtcact aatcacacct aactcattga ttaattgtga
       61 ctctaaatgt agtgttttgt cgcgttttgc agtatgttat ttaatctatt atatgaataa
      121 cataatgatt tttaatgtat ttttgtaatt tactctcctt ctgacatgga aggacacccg
      181 atggataccc aacgattcca atcccaattt cattggcatt tatcgtttaa attttctggc
      241 gcgatagcag cgtgcttgtc cctgtctctt gtgggaacag ggcttgctaa tgctgatgat
      301 tcgcttcctt ccagtaacta tgcgccgccc gccgggggaa cattcttttt gcttgctgac
      361 agcagtttta gcagcagtga agaggcgaaa gtgcgactgg aagcgccggg gcgtgattat
      421 cggcgctatc agatggaaga gtacggcggc gtggacgttc gcctgtatcg tattcctgac
      481 ccgatggcat ttttgcgcca gcagaaaaac ctgcatcgca ttgtggtgca accgcaatat
      541 ctgggcgacg ggctgaacaa tacgctgacc tggctgtggg ataactggta cggcaaatct
      601 cgccgcgtaa tgcagcgtac tttctcttct cagtcacggc agaatgtgac tcaggcatta
      661 cccgaattac atctcggcaa tgccattatt aaaccttccc gttatgtaca gaacaaccag
      721 ttttccccgc tgaaaaaata tcccttggtg gaacagttcc gttatccact atggcaggct
      781 aaaccggtcg agccgcagca aggggtaaaa ctggaaggcg catccagcaa tttcatctcg
      841 ccgcagccgg gtaacattta tattcctctc ggccaacaag agccgggact gtacctcgtc
      901 gaggcgatgg ttggtgggta tcgggcgacg acggtggtgt ttgtttccga taccgtggcg
      961 cttagcaaag tgtcaggcaa cgagcttctg gtgtggaccg cgggtaaaaa acagggtgaa
     1021 gcgaagcccg gctcagagat cttgtggact gacggtcttg gcgtgatgac ccgcggtgtg
     1081 accgatgaca gcggtacctt gcagttacaa catatatcgc cagaacgttc atacattctg
     1141 ggtaaggatg ctgaaggcgg tgtttttgtc tccgagaact tcttctacga aagcgaaatc
     1201 tacaacaccc gcttgtatat ttttaccgat cgcccgctat atcgcgcagg cgatcgtgtc
     1261 gatgttaaag tgatgggccg cgagttccac gatccgttgc attcatcccc catcgtcagc
     1321 gccccggcga agctttcggt gctggacgct aacggcagtc tgttgcaaac cgtcaatgtc
     1381 acgctggatg cgcgcaatgg cgggcaggga agtttccgcc tgccagaaaa tgccgtagcc
     1441 ggaggttatg agttacgtct tgcttaccgc aatcaggtct atagcagcag ttttcgcgtg
     1501 gcaaactaca tcaagccaca tttcgagatt ggtttagctc tcgacaaaaa agagttcaaa
     1561 actggcgaag cggtcagcgg caaactgcaa ctgctttacc cggatggtga gccggtaaaa
     1621 gatgcccgcg tgcagttaag tttgcgcgct cagcaattat caatggtcgg taacgatttg
     1681 cgttatgccg gacgttttcc cgtgtcgctg gaaggcagcg aaacggtgtc cgacgccagc
     1741 ggtcatgtgg cgttaaatct ccccgccgcc gataaaccga gccgctattt gttaaccgtc
     1801 tccgccagtg acggcgcggc gtatcgcgtc accaccacca aagagatcct cattgaacgc
     1861 ggcctggcgc attactcttt aagtaccgcc gcacaataca gtaatagcgg cgagtcggtt
     1921 gtgttccgtt atgccgcgct ggaatcttca aaacaggttc ctgttacgta tgaatggttg
     1981 cgtctcgaag accgcacgag ccatagcgga gagctaccgt caggcggcaa atcctttacc
     2041 gtcaatttcg ctaaacctgg caactacaat ctgacattac gcgataaaga cggcttaatt
     2101 ctcgctgggt taagtcatgc cgtcagcggt aagggcagca cggcgcatac tggtacggta
     2161 gatatcgtgg cggataaaac gctgtaccag ccaggcgaaa ctgcgaagat gctgattacc
     2221 tttccggagc caattgatga agcattattg acgctggaac gcgatcgcgt ggaacagcag
     2281 tcgctgcttt cgcatccggc aaactggctg acgttacaac gtttaaacga tacccagtat
     2341 gaagcccggg ttccagtgag caattccttt gcgcctaaca tcactttttc ggtgctgtat
     2401 acccgtaatg gtcagtacag ttttcagaac gccgggatca aagttgccgt tcctcagctt
     2461 gatatccggg tgaaaacgga caaaacccat taccagcctg gtgaactggt caatgtcgaa
     2521 ttaacctcgt cgctgaaagg taaacctgtt tctgcgcagc taacggtagg cgtggtcgat
     2581 gaaatgatct acgcgctgca accagaaatc gcgccgaata tcggcaaatt tttctatccg
     2641 ctggggcgta acaatgtgcg taccagctcc agtctgtcgt ttatcagcta cgaccaggcg
     2701 ctctccagcg agccggttgc gcctggcgca actaaccgca gcgagcggcg agtaaaaatg
     2761 cttgaacgtc cacggcgtga agaggtggat accgcggcat ggatgccgtc actcacaacc
     2821 gataaacaag gcaaagcgta tttcacgttc ctgatgcctg attcgttaac ccgctggcgt
     2881 atcaccgcgc gtgggatgaa cggcgacggg ctggtcgggc aggggcgtgc ttatctgcgt
     2941 tcggaaaaaa atctctacat gaagtggagt atgccaacgg tgtatcgcgt gggcgacaaa
     3001 ccggcggcag gactgtttat cttcagtcag caggataacg aaccggtggc gctggtgact
     3061 aaatttgcag gcgctgagat gcgccagacg ctgacgctgc acaaaggggc gaattatatt
     3121 tcgctgacgc agaacattca gcaatctggc ttgttaagtg cagaactgca acaaaatggg
     3181 caagtgcagg acagcattag cacaaaactg tcttttgtgg acaacagctg gcccgttgaa
     3241 cagcagaaaa atgtcatgct cggcggtggc gagaacgcgc tgatgttgcc cgagcaggcg
     3301 agtaatatcc ggctacaaag tagtgaaacg ccgcaggaga ttttccgcaa caatcttgat
     3361 gcgttagtcg atggaccatg gggtggcgtg atcaacaccg gtagccgtct gatcccgctc
     3421 agtctcgcct ggcgttcgct tgccgatcat caaagtgccg ccgctaacga cattcgtcag
     3481 atgattcagg ataaccgtct gcggctgatg caactggcgg ggcccggagc gcgctttacc
     3541 tggtggggtg aagatggcaa tggtgacgcc ttccttacgg catgggcatg gtacgccgac
     3601 tggcaggcca gccaggcgct cggcgtaacg caacaaccgg aatactggca gcatatgctc
     3661 gacagttatg ccgagcaggc ggataacatg ccgttattgc atcgggcgct ggtgctggcg
     3721 tgggcacagg agatgaatct gccgtgcaaa acgttgttga aagggttgga tgaagctatc
     3781 gcccggcgcg gaactaaaga tgaagatttc tctgaggaag acatccgcga tatcaatgac
     3841 agcctgatcc tcgatacacc ggaatctcca ctggcagatg cggtggcaaa cgtcttaacc
     3901 atgacgttgc tgaaaaaagc gcagttgaag tccacggtga tgccacaggt tcagcaatat
     3961 gcgtgggata aagcggcaaa cagcaatcag ccgctggcgc acacggttgt gctgctcaat
     4021 agcggtggtg acgctaccca gacggccgct attttaagtg gtttgaccgc tgagcaatcc
     4081 actattgagc gcgcgctggc catgaactgg ctggcgaaat atatggcgac aatgcctcca
     4141 gttgttttgc ctgcgcctgc gggcgcatgg gctaaacata agttaactgg agggggcgaa
     4201 gactggcgtt gggttggtca gggcgtgccg gacattctct cttttggtga cgaattatcg
     4261 ccgcaaaatg tgcaggtccg ctggcgtgag ccggcaaaaa tggctcaaca aagtaacatt
     4321 ccggtgaccg ttgaacgcca gttgtatcgg cttatccctg gtgaagaaga gatgagcttt
     4381 attctgcaac cggtgaccag caatgagatt gacagcgatg cgctgtatct cgatgaaatt
     4441 acgcttacca gcgagcagga tgcagttctg cgctatggtc aggtggaagt accgctcccg
     4501 ccgggagccg acgttgagcg cacaacatgg ggcatttcag tcaataaacc caacgccgcg
     4561 aaacagcagg ggcaattgct ggaaaaagcg cgtaatgaaa tgggcgaact ggcctatatg
     4621 gtgccggtga aagaactgac gggaacggtc actttccgcc atttgctgcg cttctcgcaa
     4681 aaagggcaat tcgttctgcc tcctgctcgt tatgtgcgtt cctatgcacc tgcgcagcaa
     4741 agtgttgcgg cagggagtga atggaccggg atgcaggtga aataagtgaa ctggcgcaga
     4801 atcgtctggc tattggcgtt ggttactctg ccaacgcttg cggaggagac gccgttacaa
     4861 ctggtgctgc gcggtgcgca gcacgatcaa ctgtatcagc tgtcgtcatc aggcgtgacc
     4921 aaagtctcag cattacctga ctcgctcacc acgccgttag ggagtttgtg gaaactgtat
     4981 gtttacgcct ggctggagga tacccatcaa ccggagcagc cctaccagtg tcgcggaaac
     5041 tccccggaag aggtttattg ttgtcaggct ggggagagca taacgcgcga taccgcgctg
     5101 gtacgttcct gtgggctcta ttttgcgccg cagcggttgc atattggcgc agatgtgtgg
     5161 gggcaatact ggcaacagcg tcaggcacct gcgtggttag cgtctcttac aacgctgaag
     5221 ccagaaacct cggtaacggt taaatcgctg ctcgattcat tagcgacgtt acctgcgcaa
     5281 aacaaagctc aggaagtctt actggatgtg gtgctggacg aggcgaaaat cggtgtcgca
     5341 tccatgctcg gtagtcgggt acgggtgaaa acctggagct ggtttgccga tgataaacag
     5401 gaaatccgcc agggtggttt tgctggctgg ttaaccgatg gtacaccgct gtgggttact
     5461 ggcagtggca ccagcaaaac ggtactaacc cgttatgcga cggtgcttaa tcgggttttg
     5521 ccagtgccga cgcaggttgc cagtgggcaa tgtgtagagg tggagctgtt tgcccgctat
     5581 ccgctgaaga aaatcacagc ggaaaagagt acgacggcag taaaacccgg cgtacttaat
     5641 gggcgctatc gtgtgacgtt tactaatggt aaccacatta cgtttgtcag ccacggtgag
     5701 acgaccttac tgagcgaaaa gggcaaactg aaattgcagt cgcatctcga tcgtgaagag
     5761 tacgttgcca gagttctgga tcgcgaggcg aaaagtacgc cccctgaggc agcgaaagcc
     5821 atgaccgtgg cgattcgcac ttttttacag caaaacgcca atcgggaagg cgattgcctg
     5881 accatccccg acagcagcgc cacgcagcgt gtttctgctt cgccagcgac cacgggggcg
     5941 cgaacgatga ccgcctggac gcaggatctc atctatgcag gcgatccggt tcattatcat
     6001 ggcagtcgag ctaccgaagg gacactttcc tggcggcaag ccacggccca ggcggggcag
     6061 ggagagcgtt acgaccagat actcgcgttt gcttatcccg acaacagcct tagccgctgg
     6121 ggagcgccac gctcaacctg ccagttatta cccaaagcga aagcctggct ggcgaaaaaa
     6181 atgccgcagt ggcggcgtat attacaaggc gagacggggt acaacgaacc agacgtgttt
     6241 gcggtctgtc gtttagtctc aggtttcccc tataccgatc ggcagcagaa acggctgttt
     6301 attcgtaact tctttacgct tcaggatcgg ctggatttaa cccatgagta tctgcacctg
     6361 gcctttgatg gttatcccac cgggcttgat gagaactata tcgaaacgct gacccgacaa
     6421 ttattaatgg actaatgcta tgcgaaagat ttttcttccg ttgttactgg tggcactttc
     6481 gcctgtcgct cacagtgaag gtgtgcagga agtcgagatt gacgcaccgc tctctggctg
     6541 gcatccggca gagggcgaag atgccagttt ttcacaatcg atcaattacc cggcgtcgtc
     6601 cgtcaacatg gcagatgatc aaaatatctc agcgcagatc cgcggcaaaa taaagaatta
     6661 tgctgcggcg ggtaaagttc agcagggtcg gctggtggtc aacggtgcca gtatgccgca
     6721 gagaattgaa tccgatggtt catttgcacg cccttatatt ttcactgaag gcagcaacag
     6781 cgtgcaggtc atcagcccgg atgggcaaag ccggcaaaaa atgcagtttt actcaacgcc
     6841 gggtacagga acgattcgtg cacgtttacg gctggttctc tcgtgggata cggacaatac
     6901 cgatctcgat ctccatgttg tcacgcccga tggcgaacac gcctggtacg gtaacaccgt
     6961 gctgaaaaac agtggtgcac tggatatgga tgtcacgacg gggtacggac ccgagatttt
     7021 cgccatgcca gcaccagttc acgggcgcta tcaggtgtat atcaactact atggtgggcg
     7081 cagcgaaacg gaattgacga ccgcccaact gacgctgatc accgatgaag gatcggtcaa
     7141 tgagaaacag gaaacattta ttgtaccgat gcgtaatgct ggcgagctga cgctggtgaa
     7201 aagttttgac tggtgatcca tcccttcaca caacctggct ctctcttttt tcagataaga
     7261 ctgctgggcg atctgccgac gccaggatat aaccattaac aagcgtcggc agattggtca
     7321 tttcttattt ttcgtactga gaactgtcca tctactccgg ctttcctggt taaggcaatg
     7381 gtttctaagc gatacgcatt attgcaatgg tgacagaaca ttgcggtaac acgcttttac
     7441 cgctacctta accacactcc atcggtcacc tgaggcggag cttcgcccct ttgaaatacc
     7501 ttgcttcttt tcgtacaacc ctgaaagcct cgcgctacat gttcagagca ttggcgttag
     7561 tgctctggct gttgattgct ttttcatccg ttttttacat cgttaatgcg ttacatcagc
     7621 gagaatcgga aattcgtcag gaatttaatc tgagttccga tcaggctcag cgctttattc
     7681 aacgcacctc tgatgtgatg aaagagctga agtacatcgc cgaaaatcgc ttatcggcag
     7741 aaaacggtgt gctttccccg cgtggacgag aaacgcaggc ggatgtgcct gcgtttgaac
     7801 cgctgtttgc cgactccgat tgttccgcaa tgagtaacac ctggcgaggt tctctggagt
     7861 cattggcgtg gtttatgcgc tactggcgcg ataatttttc tgcggcttac gatctcaacc
     7921 gggtattttt aatcggcagt gataacctct gcatggccaa tttcggtctg cgtgatatgc
     7981 cagtggaacg cgataccgcg ttgaaagcgt tgcatgaacg catcaataaa tatcgaaatg
     8041 caccacaaga tgatagcggc agtaacctct actggatcag cgaaggtccg cgccctggcg
     8101 tcgggtattt ttacgcgttg acgccagttt atctggcgaa ccggttgcag gcgcttttgg
     8161 gtgtcgagca gaccatccgg atggaaaact ttttcttacc gggtacgttg ccgatggggg
     8221 ttaccattct tgatgaaaat ggtcataccc tgatttcgct taccggacca gaaagtaaaa
     8281 ttaagggcga tcctcgctgg atgcaggaac gctcctggtt tggctatacg gaagggttcc
     8341 gggagctggt gctgaagaaa aatctgccac cctcatcgct aagcatcgtg tattcggtgc
     8401 cggttgataa ggtgctggaa cgcattcgca tgttgatcct taacgcaatt ttgctgaatg
     8461 tgcttgccgg agctgcattg tttactctcg cacggatgta cgagcgacgt attttcattc
     8521 cggcggaaag cgacgccctg cgactggaag aacatgagca gttcaatcgc aagattgtcg
     8581 cctccgcgcc agtgggtatc tgcattttgc gtaccgctga tggcgtcaat attttaagta
     8641 acgaactggc gcatacctat ctcaatatgc ttacgcatga ggaccgccaa cgactgacgc
     8701 aaattatctg tgggcagcag gtcaattttg ttgatgtcct gaccagcaac aataccaatc
     8761 tgcaaatcag cttcgtccat tcgcgctatc gtaatgaaaa cgtggccatt tgtgtgctgg
     8821 tggatgtttc ttcgcgcgtg aagatggaag agtcgttgca ggagatggca caagcagcgg
     8881 aacaggcgag ccagtcaaaa tcgatgttcc ttgccaccgt cagtcatgag ctgcgaacgc
     8941 cgctgtatgg cattatcggt aacctggatc tgttgcaaac caaagagtta ccgaaaggcg
     9001 tcgatcggct ggtgacggca atgaacaact cttccagcct gttgttgaaa attatcagcg
     9061 atattctcga tttctcgaag attgaatcgg aacagttgaa gatcgaaccg cgtgagtttt
     9121 caccgcgtga agtgatgaac cacatcaccg ccaactattt accgctggtg gtacgcaagc
     9181 agttaggctt gtactgcttt attgaaccgg atgtgccagt ggccttaaat ggcgacccga
     9241 tgcgtttaca gcaggtcatc tccaacctgc tgagtaacgc cataaaattc accgataccg
     9301 gctgtatagt tttgcatgtt cgcgctgatg gcgattatct ctctatccgt gttcgcgata
     9361 ccggcgtggg cattccggcg aaagaagtgg tgcgcttgtt tgatcccttc ttccaggtcg
     9421 gaacgggcgt acagcgtaac ttccagggga ccggtctggg tctggcgatt tgtgaaaaac
     9481 tgatcagcat gatggacggc gatatctcgg tagattcaga accgggaatg ggcagccagt
     9541 ttaccgtgcg tattccgttg tacggcgctc agtacccgca gaaaaaaggc gtggaagggt
     9601 tgagtggtaa acgctgctgg ctggcggtcc gcaatgcgtc gctctgtcag ttcctggaaa
     9661 ccagtttgca gcgcagcggc atcgtcgtta caacatacga agggcaggaa ccgactcccg
     9721 aagatgtgtt aatcactgac gaggtagtga gtaaaaaatg gcagggtaga gcggtagtga
     9781 ccttctgtcg tcgccatatt ggtattccgc tggagaaagc gccaggggag tgggtacaca
     9841 gtgtggctgc tccgcatgag ctaccggcat tgttggcgcg tatttatttg atcgagatgg
     9901 agagcgacga tcctgctaac gctctgccgt cgacggacaa agcggtcagc gataacgacg
     9961 atatgatgat tctggtcgtg gatgatcatc cgattaaccg acgtctgctg gcagatcagt
    10021 tgggatcgtt gggctatcaa tgtaaaaccg cgaatgatgg cgtcgatgcg cttaatgtac
    10081 ttagcaagaa tcatattgat atcgtgctta gcgacgtcaa catgccaaat atggatggtt
    10141 accgcttgac gcaacgcatt cgtcagttgg gactgacgtt gccggtaatc ggagtaactg
    10201 ctaatgcgtt ggctgaagag aagcagcggt gtctggagtc cggtatggac agctgcctgt
    10261 cgaagccggt aacgctggat gtgataaaac agacgctgac ggtatatgcc gagagggtca
    10321 ggaaatcgcg ggaatcgtag gacggataag gcgtttacgc cgcatccggc attcagtgca
    10381 aatgccagat aagacactaa cgcgtcttat ctggcctaca ggtgattagt ctttatctgc
    10441 cggacttaac gtcacagaag agagataatt cagcagggcg atatcgttct cgacacccag
    10501 cttcatcatc gcagatttct tctggctact gatggtttta atactgcggt tcagcttttt
    10561 agcgatctcg gtcaccagga agccttccgc aaacaggcgc agaacttcac tctcttttgg
    10621 cgagagacgc ttgtcaccgt aaccaccagc actgattttt tccaacaggc gagaaacgct
    10681 ttccggggta aatttcttcc ctttctgcag cgcggcgaga gctttcggca gatcggtcgg
    10741 tgcaccttgt ttcagcacga tcccttcgat atccagatcc aataccgcac taagaatcgc
    10801 cgggttgttg ttcatggtca gaacaataat cgacaggctt gggaaatggc gcttgatgta
    10861 cttgattaag gtaatgccat cgccgtactt atcgccaggc atggagagat cggtaatcaa
    10921 cacatgcgca tccagtttcg gcaggttgtt gatcagtgct gtagagtctt caaattcgcc
    10981 gacaacattc acccactcaa tttgctcaag tgatttgcga ataccgaaca agactatcgg
    11041 atggtcatcg gcaataatta cgttcatatt gttcatgtaa taggctacct tgctacagca
    11101 agctcttgac ataactgtca atgtcgctga tgtatttttc tattcctgga acatccttct
    11161 cacgaatcag atgttccagc gtttcacata actgcttgcc gggtaccaga tttagcatgg
    11221 caaatacgcc tttaagacga tgagccgttt gggctaacgc agcaaagtca ctggttgctg
    11281 cttcagtata cagcctctta acatcatccg gtactgtgtc tacaaagagc gcataatagc
    11341 cgctggcatg gagttgcgca ttttcatctc cgcccagagg cgattctgtc acctcttcct
    11401 gcgccagttg cacttcaatt aattgtaaga ccgcttcctg catagcgttg ctcatattga
    11461 agttgacgca caattgacca ggcccaattt cccgtacgcc agactcatca tcgcttaaaa
    11521 gcaagccaga ggcagtaaga ttagacggat tatccgttaa aaagatatca taatcttgac
    11581 taattaatct ttcatcgggt gtgatacagg ttgcacccca attttctaac tggcgagtga
    11641 caatattccg aatttctgcc gaagtaacat ccaccattac gcagacatca tccagtaaac
    11701 gctcttcttc ctcttcaact tccgggtcag cggcgagcat tttgatatgc acagagtagc
    11761 gtgtaccaag cccatcccgc gttttgatgt ttaaatgacc gcccagttta cgcgccagtt
    11821 gatcgctcag ccagaatgcc agcgggtccg ccttgccata gcgatcgttt tgggtctggt
    11881 tgataaacgg gaagtgcaaa ttatccattt catgaatact tacgccttct ccggtgtcca
    11941 gaatgcggaa tgtcaggcgg tcttcagagg actcatcctg atcaacctca agggtgattt
    12001 ttcccaattg cgttgaggtc acggcatatt gcatcagtag cagcaaaata cgtcgtaagg
    12061 catcgcgatc gccgcggcgc atatcgtgtg ctttcagatg attgttaatc agcagttgca
    12121 gacctttacg cttgatggca ggcaacactg aaggcacaac ttcatcaatt aaatcctgca
    12181 cggagaacag caccgtctca cttttccagc tatcatccgc aagcatgttc gctaactgta
    12241 tttcatcaac cagccgcacc agcacatctg cctgattcgc cagttgtttg ctttccgggg
    12301 cgttgagttt agccgcgctc tccgccaggg actgtgcagg ttctttcagc gcatcgccaa
    12361 tgtttttcat aaaggtcatc cggccctgct ggtttttctc atacagacgc tgcgcctgct
    12421 tgagtttctt gtttaccagc acttcgcgat cctgatcgcg aataatgaaa atttgtgtgc
    12481 gcggcgcgac ctggctgcgg aacatgcgga tctcatacag ctcgttattg atcgtcgcct
    12541 gaataatccc ctgatgctgt tccgccatgg tggtgatgtt ttgcagattc aaatgcggca
    12601 gcaaatgatc ggcaattttg ttacttatga cagtgcggtt cgattcctga tcgtgaacca
    12661 gcaggccgag cggcagcagt gagactatct cttcattaat tgcccgtaaa atgcgcaatt
    12721 cgttattgac cgccgtgctg ggtagacttt ctgtactgcg gctggagaaa tggcggaatg
    12781 tggtatagcc aaataacgcc agcgccagca aaccgatgtt cagcagcagt ggcagcagaa
    12841 tgttttgcaa cgtatccagc aataaggtgc cataaggaac ctgccagacc aagcgcatat
    12901 cggtagagtt gagtgccgag gagatttcaa tcttcgtact gttaaagtgg atactgacgc
    12961 tatccgtccc ttctttctca ttatcattgt ttcccgttgc cgtcgcgtct ggctcaaggc
    13021 ggaaactgtc cagcggcata cccggtggga tcaaatcatt aatcggcaga tcaaaagcca
    13081 cgaccgttgc cagatgtcct ggctggttaa aggtagtacg caaggtaaag taatgaccgt
    13141 tctgccaggc caggcggcgc atgttagaaa agctttcgcg ttcatcgagg gcgttggcct
    13201 gttgcaacat ctctgcacga cgtgaatcaa caatgtcgct gacggtcgat tctttaaatc
    13261 cggaggtgag atctttaagg ggtagggttg agatcagcac cagactgtta tcctgaccat
    13321 tcaggtaata catcgaccac ggtacatttt ctgcgcccca caatgtatcc agataagtgg
    13381 acatccgctg agtcatctca agcgttgagt tgtcgtgaga gccaaagatt aacgcttccg
    13441 ttttgcggcg tggtttctcc agatagtaga catcctgttt caggcgcgtc tcttgtaaac
    13501 cttcgccgga ggagggggag gtcgtcgcgg caatgttgtc gtagatctgc caggtcacgt
    13561 aacgccaggt atcgacgcgc ttttggatag cgtgggtaat gtcgacaatc tggtaacttt
    13621 tatctttcag ccatgcgtta acggcgcttt gtaccattac acccatcgtc accagtaaca
    13681 caatgatcaa cagtaaaaag aagcgggtaa tgctccccgg taggagtgaa aagcgggtcg
    13741 tggccgttgt ctctttctga cgcattcgtg tttatgacct gttaaaactt cgcgaagtga
    13801 tagggcagta taaagggtac agtgtgaaat tccagactct tacctgattg atgattctct
    13861 attctcccgt gatatttatc atcctgcatt gggggaaata gatgtacaag cgccattttg
    13921 ctgtacaaat aacctacaaa aagcccaacg aaaataattg caaataaaca aagattgctg
    13981 gaaattatgc tgatgttaat tatttgtgaa atagttaaca agcgttatag tttttctgtg
    14041 gtagcaaaga ataatgaaaa gtgtgtaaag aagggtaaaa aaaaccgaat gcgaggcatc
    14101 cggttgaaat aggggtaaac agacattcag aaatgaatga cggtaataaa taaagttaat
    14161 gatgatagcg ggagttattc tagttgcgag tgaaggtttt gttttgacat tcagtgctgt
    14221 caaatactta agaataagtt attgatttta accttgaatt attattgctt gatgttaggt
    14281 gcttatttcg ccattccgca ataatcttaa aaagttccct tgcatttaca ttttgaaaca
    14341 tctatagcga taaatgatac atcttaaaag ttttagtatc atattcgtgt tggattattc
    14401 tgcatttttg gggagaatgg acttgccgac tgattaatga gggttaatca gtatgcagtg
    14461 gcataaaaaa gcaaataaag gcatataaca gagggttaat aacatgaaag ttaaagtact
    14521 gtccctcctg gtcccagctc tgctggtagc aggcgcagca aacgctgctg aagtttacaa
    14581 caaagacggc aacaaattag atctgtacgg taaagtagac ggcctgcact atttctctga
    14641 caacaaagat gtagatggcg accagaccta catgcgtctt ggcttcaaag gtgaaactca
    14701 ggttactgac cagctgaccg gttacggcca gtgggaatat cagatccagg gcaacagcgc
    14761 tgaaaacgaa aacaactcct ggacccgtgt ggcattcgca ggtctgaaat tccaggatgt
    14821 aggttctttc gactacggtc gtaactacgg cgttgtttac gacgtaactt cctggaccga
    14881 cgtactgcca gaattcggtg gcgacactta cggttctgac aacttcatgc agcagcgtgg
    14941 taacggtttc gcgacctacc gtaacactga cttcttcggt ctggttgacg gcctgaactt
    15001 tgctgttcag taccagggca aaaacggtag cgtaagcggc gaaggcatga ctaacaacgg
    15061 tcgtgaagca ctgcgtcaga acggcgacgg cgttggcgga tctatcactt atgattacga
    15121 aggcttcggt atcggtgctg cagtttccag ctccaaacgt actgatgatc agaacagccc
    15181 gctgtacatc ggtaacggcg accgtgctga aacctacact ggtggtctga aatacgacgc
    15241 taacaacatc tacctggctg ctcagtacac ccagacctac aacgcaactc gcgtaggttc
    15301 cctgggttgg gcgaacaaag cacagaactt cgaagctgtt gctcagtacc agttcgactt
    15361 tggtctgcgt ccgtccctgg cttacctgca gtctaaaggt aaaaacctgg gtcgtggcta
    15421 cgacgacgaa gatatcctga aatatgttga tgttggtgcg acctactact tcaacaaaaa
    15481 catgtccacc tatgttgact acaaaatcaa cctgctggac gacaaccagt tcactcgtga
    15541 cgctggcatc aacactgata acatcgtagc tctgggtctg gtttaccagt tctaatctcg
    15601 attgatatcg aacaaagggc ctgcgggccc ttttttcatt gttttcagcg tacaaacaca
    15661 gttttttggt gtactcttgc gaccgttcgc atgaggataa tcacgtatgg aaataagctt
    15721 tacccgcgtg gcactgctgg ctgccgcgct cttctttgtt ggttgcgatc aaaaaccaca
    15781 aaccgccaaa acccacgcta ctgaagttac cgttcttgaa ggcaaaacta tgggtacctt
    15841 ctggcgtgcc agcatcccgg gcattgacgc caaacgcagt gccgaactta aagaaaagat
    15901 tcagacccag ctggacgccg acgatcagct gctttcgacc tataaaaaag attccgcgct
    15961 gatgcgcttt aacgattcgc aaagtttatc gccgtggccg gtaagtgaag cgatggccga
    16021 tatcgtcacc acctcgctgc gcattggcgc aaggaccgat ggtgcgatgg atataaccgt
    16081 cgggccgctg gtgaatctgt ggggctttgg cccggaacaa cagccggttc aaattccgag
    16141 ccaggaacag atcgatgcga tgaaagccaa aaccggctta cagcacctga cggtcattaa
    16201 tcagtcgcat cagcaatatc tgcaaaaaga cctaccggat ttatatgtcg atctctccac
    16261 cgtcggtgaa ggttatgcgg cggatcacct ggcacgcttg atggagcagg aagggatttc
    16321 ccgctatctg gtgtcggtgg gcggcgcgct gaacagccgt ggtatgaacg gtgaaggcct
    16381 gccgtggcgg gtagcgattc aaaaaccaac cgataaagaa aacgcggttc aggctgtggt
    16441 ggatatcaac ggtcatggta ttagcacctc tggcagttac cgtaactatt acgaactgga
    16501 cggcaaacgt ctttctcatg ttatcgatcc gcaaaccggg cgtcccatcg aacacaatct
    16561 ggtatccgtg acggtgattg ctccgacggc gctggaagcc gatgcctggg atactggctt
    16621 gatggtactc gggccggaga aagccaaaga ggttgttcgc cgggaagggc tggcggttta
    16681 tatgatcacc aaagaaggcg atagctttaa aacctggatg tcaccacagt ttaaaagctt
    16741 ccttgtcagc gaaaaaaatt aaagcgcaag attgctggtt tttgcgtgat ggtgaccggg
    16801 cagactaaag gctatcctta accagggagc tgattatgaa aaacgccaca tgcttaactg
    16861 acgatcaacg ctggcaatct gtcttagccc gcgacccgaa tgccgacagc gaattcgttt
    16921 tcgccgtgcg caccacaggc atcttttgcc gtccgtcttg ccgcgccaga catgccttgc
    16981 gggaaaatgt ctccttctac gcaaatgcct gcgaggcact cgccgccgga tttcgcccct
    17041 gcaaacgctg ccaacctgat aaagccaatg cccagcaaca tcggttggat aaaatcaccc
    17101 acgcgtgtcg actgctggaa caggaaacgc ctgtaacgct ggaagcctta gccgaccagg
    17161 tggcgatgag tccatttcat ctacatcggt tgtttaaagc gactaccgga atgacgccta
    17221 aagcctggca acaggcctgg cgcgctcgcc gtttgcgcga atcgctggcg aaaggggaga
    17281 gcgtgacgac gtctattctt aacgccggat tccccgacag cagcagttac tatcgcaaag
    17341 ctgacgaaac gctgggcatg acggctaaac aattccgtca cggtggcgaa aatctggcgg
    17401 tgcgttacgc gctggctgat tgtgagctgg gtcgttgcct ggtggcagaa agcgagcggg
    17461 ggatttgcgc gatattgctg ggcgatgatg acgcgacact aatcagcgag ttgcagcaga
    17521 tgtttcccgc tgccgacaac gcgcctgccg atctgatgtt tcagcaacat gtgcgtgaag
    17581 tgatcgccag cctcaatcaa cgcgatacgc cgctgacgtt accgctggac attcgcggca
    17641 ctgcttttca gcaacaagtc tggcaggcac tgcgcacgat accttgcggt gaaaccgtca
    17701 gttatcagca actggctaac gccatcggca aaccgaaagc ggtacgggcc gttgccagcg
    17761 cctgtgccgc caacaagctg gctatcataa taccctgtca tcgggtggtc cgtggtgatg
    17821 gcacactttc cggttaccgc tggggcgtgt cgcgtaaagc gcaactgctg cgccgcgaag
    17881 ctgaaaatga ggagaggtaa tgttggatct gtttgccgat gctgaaccgt ggcaagagcc
    17941 actggcggct ggagcggtaa ttttgcaccg ttttgctttt aacgctgcgg agcaactgat
    18001 ccgcgatatt aatgacgttg ccagccagtc gccgtttcgc cagatggtca cccccggggg
    18061 atataccatg tcggtggcga tgaccaactg tgggcatctg ggctggacga cccatcggca
    18121 aggttatctt tattcgccca ttgatccgca aacaaataaa ccgtggcccg ccatgccaca
    18181 aagctttcat gatttatgtc aacgtgcggc tacggcggcg ggctatccgg atttccagcc
    18241 agatgcttgt cttatcaacc gctacgctcc tggcgcgaaa ctgtcgctgc atcaggataa
    18301 agacgaaccg gatctgcgcg cgccgattgt ttctgtttct cttggcttac ccgcgatttt
    18361 tcaatttggt ggcctgaaac gtaatgatcc gctcaaacgt ttgttgttgg aacatggcga
    18421 tgtggtggta tggggcggtg aatcgcggct gttttatcac ggcattcaac cgctgaaagc
    18481 ggggtttcat ccactcaccg gcgactgccg ctacaacctg acattccgtc aggcaggtaa
    18541 aaaagaataa aaataagaat tattattgct gtgcgcgcga agatgtttaa actgcgggct
    18601 gtaattcatt gtccgggttt tctgcatgga acttcttgta cttgtctggc ggcagtatcg
    18661 ctggccattt atcagtgtga tggcgctaag cctcgccagt gcggcattag gcattggctt
    18721 aattgctttt atcaatcagc gccttatcga aacggcggat accagtctgc tggtattgcc
    18781 ggagtttctg ggattattgc tgctgttgat ggcagtcact ctcggatcgc aactggcgct
    18841 caccactttg gggcatcact tcgtttaccg actgcgtagt gaatttatca agcggattct
    18901 ggataccaac gtcgagcgca ttgaacaact cggtagcgcc tcgttgctgg cgggattaac
    18961 cagcgatgtg cgcaatatca ccattgcttt tgtgcgtctt ccggaactgg tgcaggggat
    19021 cattctcact atcggttccg cggcgtatct gtggatgctg tcgggcaaaa tgttgctggt
    19081 aacggctatc tggatggcga tcaccatctg gggtggtttt gtactggtgg cgcgggtgta
    19141 caaacatatg gcgaccctgc gtgaaaccga agacaggctg tatacggatt ttcaaactgt
    19201 actcgaaggg cgcaaagagc tgactctgaa ccgggaacgc gccgagtatg tgtttaacaa
    19261 cctctacatt cctgatgcgc aagagtatcg ccaccatatt atccgcgcag acaccttcca
    19321 tcttagcgcc gtgaactggt caaacatcat gatgttgggc gcaatcggcc tggtgttctg
    19381 gatggcgaac agtctcggtt gggctgatac caacgttgcc gcgacctatt cgttgacgct
    19441 tttattcctg cgtacgccgc tgctttcggc ggttggcgca ttgccgacgc tgctgacggc
    19501 gcaggtggcg tttaacaagc tgaacaaatt cgcgctcgcg cctttcaaag cagagtttcc
    19561 gcgcccgcag gcgtttccca actggcaaac gctggagctg cgtaacgtga cgtttgctta
    19621 tcaggataac gcgttttccg ttggtccgat taatctcacc atcaaacgtg gcgagctgct
    19681 gtttctgatt ggcggcaacg gtagcggaaa atcgacgctg gcgatgttgt tgacgggctt
    19741 gtatcagcca caaagcggcg aaatcttgct ggatggcaaa cctgtcagcg gcgaacaacc
    19801 ggaagattat cgcaaactgt tttcggcagt gtttaccgat gtctggctgt ttgatcaact
    19861 gctggggccg gagggtaaac ccgctaaccc gcaactggtt gagaagtggc tggcgcagct
    19921 gaaaatggct cataagcttg agttaagcaa cgggcgtatt gttaacctga agttatcaaa
    19981 agggcagaaa aaacgcgtgg cgctgttgct ggcgctggca gaagaacgcg atattatcct
    20041 gctggatgaa tgggcggcgg atcaggatcc acacttccgt cgtgagtttt atcaggtgtt
    20101 gctgccgctg atgcaggaga tgggtcaaac tattttcgcc atcagtcatg atgatcatta
    20161 ctttatccac gccgaccgcc tgctggaaat gcgcaacggg caacttagcg agctgacggg
    20221 cgaagagcgc gatgccgctt cgcgtgatgc cgttgcccgg acggcataat attacgacgc
    20281 tgacctggcg gactgctgcc gtcaggtcaa tatgctaaa
//
LOCUS       DAZGLJ010000068        20307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000068 DAZGLJ010000000
VERSION     DAZGLJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.068"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            52..456
                     /gene="yaaI"
                     /locus_tag="ACHK49_002188"
     CDS             52..456
                     /gene="yaaI"
                     /locus_tag="ACHK49_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO9262833.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            482..1195
                     /gene="msyB"
                     /locus_tag="ACHK49_002189"
     CDS             482..1195
                     /gene="msyB"
                     /locus_tag="ACHK49_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO9262834.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELAELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            1344..1910
                     /gene="satP"
                     /locus_tag="ACHK49_002190"
     CDS             1344..1910
                     /gene="satP"
                     /locus_tag="ACHK49_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO9262835.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(1945..2532)
                     /gene="mog"
                     /locus_tag="ACHK49_002191"
     CDS             complement(1945..2532)
                     /gene="mog"
                     /locus_tag="ACHK49_002191"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO9262836.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2647..3600)
                     /gene="tal"
                     /locus_tag="ACHK49_002192"
     CDS             complement(2647..3600)
                     /gene="tal"
                     /locus_tag="ACHK49_002192"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO9262837.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            3879..5309
                     /gene="yaaJ"
                     /locus_tag="ACHK49_002193"
     CDS             3879..5309
                     /gene="yaaJ"
                     /locus_tag="ACHK49_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO9262838.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     IQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            5379..6155
                     /gene="yaaA"
                     /locus_tag="ACHK49_002194"
     CDS             5379..6155
                     /gene="yaaA"
                     /locus_tag="ACHK49_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO9262839.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(6308..6604)
                     /gene="yaaX"
                     /locus_tag="ACHK49_002195"
     CDS             complement(6308..6604)
                     /gene="yaaX"
                     /locus_tag="ACHK49_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO9262840.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(6818..8104)
                     /gene="thrC"
                     /locus_tag="ACHK49_002196"
     CDS             complement(6818..8104)
                     /gene="thrC"
                     /locus_tag="ACHK49_002196"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO9262841.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(8105..9037)
                     /gene="thrB"
                     /locus_tag="ACHK49_002197"
     CDS             complement(8105..9037)
                     /gene="thrB"
                     /locus_tag="ACHK49_002197"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO9262842.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAEIF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(9039..11501)
                     /gene="thrA"
                     /locus_tag="ACHK49_002198"
     CDS             complement(9039..11501)
                     /gene="thrA"
                     /locus_tag="ACHK49_002198"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO9262843.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDRFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(11528..11647)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(11583..11648)
                     /gene="thrL"
                     /locus_tag="ACHK49_002199"
     CDS             complement(11583..11648)
                     /gene="thrL"
                     /locus_tag="ACHK49_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO9262844.1"
                     /translation="MKRISTTIITTITITTGNGAG"
     gene            complement(11862..12548)
                     /gene="yjtD"
                     /locus_tag="ACHK49_002200"
     CDS             complement(11862..12548)
                     /gene="yjtD"
                     /locus_tag="ACHK49_002200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO9262845.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(12948..13088)
                     /gene="yjjY"
                     /locus_tag="ACHK49_002201"
     CDS             complement(12948..13088)
                     /gene="yjjY"
                     /locus_tag="ACHK49_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO9262846.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            13184..13900
                     /gene="arcA"
                     /locus_tag="ACHK49_002202"
     CDS             13184..13900
                     /gene="arcA"
                     /locus_tag="ACHK49_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO9262847.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(13960..15312)
                     /gene="creD"
                     /locus_tag="ACHK49_002203"
     CDS             complement(13960..15312)
                     /gene="creD"
                     /locus_tag="ACHK49_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO9262848.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(15370..16794)
                     /gene="creC"
                     /locus_tag="ACHK49_002204"
     CDS             complement(15370..16794)
                     /gene="creC"
                     /locus_tag="ACHK49_002204"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO9262849.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(16794..17483)
                     /gene="creB"
                     /locus_tag="ACHK49_002205"
     CDS             complement(16794..17483)
                     /gene="creB"
                     /locus_tag="ACHK49_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO9262850.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQAPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTIDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(17496..17969)
                     /gene="creA"
                     /locus_tag="ACHK49_002206"
     CDS             complement(17496..17969)
                     /gene="creA"
                     /locus_tag="ACHK49_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO9262851.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            18180..19049
                     /gene="robA"
                     /locus_tag="ACHK49_002207"
     CDS             18180..19049
                     /gene="robA"
                     /locus_tag="ACHK49_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO9262852.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(19046..19693)
                     /gene="gpmB"
                     /locus_tag="ACHK49_002208"
     CDS             complement(19046..19693)
                     /gene="gpmB"
                     /locus_tag="ACHK49_002208"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO9262853.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            19745..20266
                     /gene="yjjX"
                     /locus_tag="ACHK49_002209"
     CDS             19745..20266
                     /gene="yjjX"
                     /locus_tag="ACHK49_002209"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO9262854.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
BASE COUNT         4879 a         5433 c         4998 g         4997 t
ORIGIN      
        1 gtaaactagt gggtatccat ccccctacct ctccccacta agagaatcct tatgaaatcc
       61 gtttttacga tttccgccag cctggcgatt agtctgatgc tgtgctgcac ggcgcaggca
      121 aacgaccata aaatcctcgg cgtcattgcg atgccacgta acgaaaccaa cgatctggcg
      181 ctgaaacttc ctgtttgccg aattgttaaa cgcatacaac tctctgccga ccatggcgat
      241 ttacagttaa gcggtgcatc ggtttatttc aaagccgccc gtagcgccag tcagagcctg
      301 aatattcctt cagaaataaa agaagggcaa accactgact ggatcaacat taacagcgat
      361 aacgacaata aacgctgcgt ctcaaaaatc accttttccg gtcatacggt gaactcatcg
      421 gatatggcca cgctgaaaat tatcggcgac gactaacgcc agaatataga agccacaaaa
      481 aatgaatgtt aattacctga atgattcaga tctggatttt ctccagcatt gcagtgagga
      541 acagttggca aatttcgccc gattgctcac ccataatgaa aaaggcaaaa ctcgcctctc
      601 cagcgtactg atgcgcaacg aactgtttaa atctatggaa gggcatcccg agcaacatcg
      661 ccgcaactgg cagctgattg ctggagaatt acagcatttt ggtggcgata gtatcgccaa
      721 caaactgcgc ggacacggta aattgtatcg ggccattttg ctcgatgttt caaaacgatt
      781 gaagctgaaa gccgacaaag agatgtctac gtttgagatt gagcaacagt tactggaaca
      841 atttctgcgt aacacctgga agaatatgga cgaggaacat aaacaggagt ttctgcacgc
      901 ggtcgatgcg agggtgaatg agctggcaga gctgctgccg ctgctgatga aagacaaatt
      961 attggcaaaa ggcgtgtcgc atttgctttc cagccaactg acccgcattt tacgcaccca
     1021 cgcagcaatg agcgtacttg ggcatggttt gctgcgcggc gcggggctgg gaggccctgt
     1081 aggtgcggca ctaaatgggg ttaaagcggt cagcggcagc gcctatcgcg tgacgattcc
     1141 agccgtactg caaatcgcct gcctgcgccg gatggttagc gccactcagg tctgataccg
     1201 gaaaccgggg aaaattttca ttttcccctc aaaagatcgt agacactgcc ccactggctg
     1261 attattatgc cgcgccctga aaacactaca gttattcagg gaaattattt caccattcat
     1321 tcgatgatga tttttgagga attatgggca acactaagtt ggctaatccg gcaccgctgg
     1381 gcctgatggg cttcggcatg accaccattc tgcttaacct gcacaacgtg ggttatttcg
     1441 ctctggacgg tattattctt gccatgggca ttttctacgg cggcatcgcg caaatttttg
     1501 ccggtctgct ggagtacaaa aaaggcaaca ctttcggttt aaccgcattc acctcttacg
     1561 gttctttctg gctgacgttg gttgcgattc tgctgatgcc gaaactgggt ctgaccgatg
     1621 cgccaaatgc acagttcctt ggtgtctacc tgggtctgtg gggcgtattt acgctgttta
     1681 tgttcttcgg cacgctgaaa ggcgcacgcg ttctgcaatt cgttttcttt agcctgactg
     1741 tgctgtttgc cctgctggcg atcggtaaca ttgccggtaa cgccgcaatc atccactttg
     1801 ccggctggat tggtctgatc tgtggtgcca gtgcaatcta tctggcgatg ggtgaagtac
     1861 tgaacgagca gtttggtcgc accgttctgc cgattggtga atcccactaa ttgttttgag
     1921 atccccccgc tcggggggat ttttttattc gctaacgtcg cgtcttgcac tcttcggtct
     1981 gaatgcagca accacttctg gtgccgtttc aacgtatggc ccttccagca actgaatgca
     2041 gtacggtacg ctggcaaaaa taccgtgtac cacaacgtta cccgcagcgt ccttcacacc
     2101 ttccagcgtc tctttaatag acttcggctg accgggtaag ttaaggatca gcgcctgttt
     2161 gcgaatcaca ccaacctgac gcgaaaggat cgcagttggt acaaaatgca ggcttatctg
     2221 gcgcatctgt tcaccaaagc caggcatctc gcggtccgct actgccagcg tcgcatcggg
     2281 cgttacgtca cgacgcgccg ggccagttcc gcccgtggtg agcaccagat ggcaactcat
     2341 ttcatccact agctcacaca acgtttgctc gatgatcgcc tgctcatcgg ggattaagcg
     2401 ggtttccagt tcaaacggcg tggttagcgc cgatgtcagc cattcttcca gcgcagggat
     2461 gcctttatcc tgataaacgc cgctggatgc gcgatcagag atggaaacta agccaatacg
     2521 taaagtattc atcaagattt ttccgtttaa acagtctcgt taaacagaat gatacactgc
     2581 gaagggagtg acagacaggc ttcagaagag gtagcgtgac cgacttcccg gtcacgctaa
     2641 gaatgattac agcagatcgc cgatcatttt ttccagtttt tcctggtcaa tagcaaactt
     2701 acggatacct tccgccagtt tatctactgc cattggatcc tggttgtgct gccacaggaa
     2761 ctcggactca gtgatacgcg ccggacgcgc tttcacttcg ccggtgtaag acagtttacg
     2821 ttcgatagcc ccttcgctct cagccagctc tttcagcagt gtaggtgcga tggtaagacg
     2881 gtcgcagcct gccagttcca gaatttcgcc gatgttacgg aagcttgcgc ccataaccac
     2941 ggtttcataa ccgtgctctt tgtagtactg gtagatttca gatacagaaa ccacgcccgg
     3001 atcttctgcc ggagcgtact ctttcttatc ggtattcgct ttgtaccagt caagaatacg
     3061 gccaacaaac ggcgagatca ggaacacgcc cgcttccgca caagcacgag cctgagcgaa
     3121 ggagaacagc agggtcaggt tacagttgat gccttctttt tccagctgtt ctgcagcacg
     3181 gataccctgc caggtagaag ccagtttgat cagaatacga tcgttgctaa taccagcatc
     3241 gttgtagagt ttgatcaggc gttttgcttt cgcaattgac gcttcggtgt cataggaaag
     3301 acgcgcatca acttcagttg agatacggcc cggaaccagt ttcaggattt ccagaccaat
     3361 atttactgcc agtttgtcgg tcgcgtccac gatctgctgc gcgcgatcgt tgctctgctg
     3421 tttcgcccag gcgacagcat catcaatcaa cttacggtat tccggaatct gcgctgcgtt
     3481 aagaatgaga gaagggttgg ttgtggcatc ctgcggttga tacagcttca ttgccgcgat
     3541 gtccccagtg tcggccacta cggtggtgta ctgacgaagg gaggtcaatt tgtccgtcat
     3601 gatagtattt ctctttaaac agcttgttag ggggatgtaa ccggtctgcc ctgatgatat
     3661 cacgacgcaa cgtcagcgca accgccgaca agacggttat cgccaggcga gactgtttcg
     3721 gatttctgac actgcgttga tatcgcccgc catttttata caaaacctca tgtatgctac
     3781 gcagaagtta tcaagtacct cgtagcgtat atacttctta aacaattggt aacgtttaca
     3841 caggaaagtc atcgcgaccg gcaataagag ggatatgcat gccagatttt ttctccttta
     3901 ttaacagcgt cctttgggga tcggtaatga tttacctgct cttcggcgca ggttgttggt
     3961 tcacttttcg caccggattt gtgcagtttc gctacatccg ccagtttggc aaaagtctta
     4021 aaaatagcat tcatccacag ccaggcggtt taacctcatt tcagtcattg tgtaccagtc
     4081 ttgcggcgcg cgtgggtagc ggcaacctgg ccggcgttgc gctggcaatt accgccggtg
     4141 gacctggagc cgtcttctgg atgtgggttg ccgcgtttat cggcatggcg acctcgtttg
     4201 ccgaatgttc cctcgcacag ctttataaag aacgtgatgc caatgggcag tttcgtggcg
     4261 gaccggcatg gtatatggcg cgcgggctgg ggatgcgctg gatgggcgtt ctgttcgccg
     4321 tctttttgct catcgcctac ggcataattt tcagcggaat tcaggcgaac gccgttgcgc
     4381 gagccctgag tttttctttt gattttcccc cgctggtgac aggtattatt ctcgctgtct
     4441 ttgctctgct ggcgatcact cgcggtcttc atggcgtcgc ccggctcatg caggggtttg
     4501 tcccgttgat ggcgataatc tgggtactga ccagcctggt gatttgcgta atgaatatcg
     4561 ggcaacttcc ccacgtcatt tggtctattt ttgagagtgc ttttggctgg caggaagcgg
     4621 caggcggcgc ggcgggatat accttaagcc aggcgattac taacggtttt cagcgcagta
     4681 tgttttccaa tgaggcggga atggggtcga cgccaaacgc ggcagcggca gcggcgtcct
     4741 ggcctccgca tccggcagcg caagggattg tccagatgat tggcattttt atcgacaccc
     4801 tggtcatctg tacggcaagc gccatgctga tattactggc gggtaacggc acaacctaca
     4861 tgccgctgga aggtattcag cttatccaga aggcgatgcg ggtgctaatg ggttcctggg
     4921 gtgctgagtt tgttaccctc gtggttattc tgtttgcctt cagctccatc gttgccaact
     4981 acatttacgc cgaaaacaat ctcttctttt tacgcctgaa caaccctaaa gcgatctggt
     5041 gtttgcggat ctgcaccttc gcaacggtca tcggcggcac cttgctaagt cttccgctga
     5101 tgtggcaact ggcagatatc ataatggcct gcatggctat taccaatttg accgccattt
     5161 tactgctctc gcctgtggtt cataccattg ccagtgatta tctacgccag cgtaaactcg
     5221 gcgtgcgccc ggtgtttgat ccgttgcgtt atccggatat cggccgccag ctttctccgg
     5281 acgcgtggga tgatgtttcg caggagtaat cacaactatc gatcaactca ttctcttttt
     5341 ttgctaaagt cggcataaat ttcctgcaag gactggatat gctgattctt atttcacctg
     5401 cgaaaacgct tgattaccaa agcccgttga ccaccacgcg ctatacgctg ccggagctgt
     5461 tagacaattc ccagcagttg atccatgaag cgcggaaact gacgcctccg cagattagca
     5521 cgctgatgcg catcagcgac aaactggcgg gtatcaacgc cgctcgcttt catgactggc
     5581 agccagattt cacgccggag aatgcccgcc aggcgattct ggcgtttaaa ggtgatgtct
     5641 acaccggctt gcaggccgaa accttcagcg aagacgattt cgattttgcc caacagcatt
     5701 tgcgaatgct ttccggcttg tatggcgtac tgcgcccgct cgatttaatg cagccttatc
     5761 gtctggaaat ggggatccgt cttgagaatg cccgagggaa agatctgtat caattctggg
     5821 gagatatcat caccaacaag ctgaacgagg cgctcgcagc acaaggcgat aatgtggtga
     5881 ttaatctggc gtcagatgag tattttaaat cggtgaagcc gaagaaattg aatgccgaga
     5941 ttatcaagcc ggtgttcctc gatgagaaga acggcaaatt taagataatc agcttctacg
     6001 ctaagaaagc acgcggtctg atgagtcgtt ttattattga aaatcggctg accaaaccag
     6061 agcaactgac tggttttaat agcgaaggtt acttctttga tgaagcttcc tccagcaatg
     6121 gcgaactggt gtttaaacgc tacgagcagc gttaagtttg cagtcaatgc cggatgcggc
     6181 gtgaacgcct tatccggcct acaaaagcat gcagattcaa tatattgcag aattcacgta
     6241 ggcctgataa gacgcgccag cgtcgcatca ggcgctgaat gccggattgt tacccggcat
     6301 ttgtcattta gcggtgatgt ttgcctggac catgaccgcc gtgatgatca tgatgagctt
     6361 tcttatggtg acgcggcggt ggcggcggtc cgtgtgggtg ccagcgattg cctcgccatt
     6421 cataatgttg tttccaccag ccgtggtcgc gccagtgacc gccatcccag taataaccgc
     6481 gattatcacg atcgcctatc tgtaatttta ctgacgggac taacgtaatt tccgcagcct
     6541 gcgctgccat gggagcgacc agaaccaggg aaagtgcgag tacgatagat tgcatctttt
     6601 tcacaggtta ctccttttat cattgcccgt tatgggtcga tatccggaga ttacgcgatg
     6661 agaacggcgg ttgttattct ccgcaatcct aatctctaag ttaccgcatt tattgagaat
     6721 ttctcctttt tccctgtcgt ttctctccat attttcttca caaaaaagcc gggctgcata
     6781 aaagcaaacc cggcctgatt gagataatga atagatgtta ctgatgattc atcatcaatt
     6841 tacgcaacgc agcaaaatcg gcgggcagat tatgtgaaag caagggtaaa tcagcacgtt
     6901 ctgccagctc ttttggcaga tccaacgttt caccgagaat cgcttccacg ctctctttaa
     6961 atttcgccgg atgcgcggtg ccgaggaaca agccatattc gcctggattc aactggtcac
     7021 gcagcgcacg atacgctacg gcagcgtgcg gctccgaggt gtagcccagt tcttttaact
     7081 cacgcattgt ctgttgcgtg gtttcatcat ccacggctgc ataacccagc tctttcagtt
     7141 gccagatttt gcggcggaac aactcttcca cacgcggcca gttgttcggc tgactcacgt
     7201 ccatcgcgtt ggataacgtc gcctgagtcg ctttggggga ccactgaccg tcgtgcagga
     7261 aacgtggcac ggtatcgttc acgttggtcg cagcaataaa acgtttcacc ggcagaccga
     7321 gtgacttcgc cagcagaccc gccgtcaaat cgccgaagtt tccgcttggt accgaaacaa
     7381 ccagctggtt gcgcgcctcc tgcggcagct gcgcaacagc ttcaaagtag tagcaaatct
     7441 gcgccagcaa acggctgatg ttaatcgagt tagctgagtt taaccccagc gccactttca
     7501 gttcttcatc atcaaacgcc tgcttcacca gcgcctgaca ggcatcgaaa tcgccgtcga
     7561 tggcaacagt ttcgatattg ccgcccaatg tacagaacag tttttcttgc agtggactga
     7621 ttttgcctcg tggatagagg ataaccactt tcacattcgg caaaccgtag aaagcatgag
     7681 ccactgccgc tccggtatca ccggaggtcg cggtcagaat ggtcactggc ttatcacccg
     7741 caatatgggt cagcatttgt gccataaagc gaccgccgaa atctttaaat gccagcgttg
     7801 gcccgtggaa caattccaga caaccgacat cgctttcaac attggcgacc ggagccggga
     7861 aggcaaacgc cgcgcgcacg cgctcttcca ggatttcctg cgggatttca tcaccaataa
     7921 acgccgagag gatcttcgca ctgcgggtga caaaatccag cttcagcatc tcatcaattt
     7981 cagtcaggct gaattccggc aggtcatgcg gaaaaaacag cccctgattt ttgcccaacc
     8041 cctgggttac ggcttgcgca aagctgacct gctcgttgtg atctttcaga ttgtagagtt
     8101 tcatttagtt ttccagtact cgtgcgcccg ccgtatccag ccggcaaata tgaacaaaac
     8161 cttcctgatt ttgcaggtag ttcttaccca accagtcggc aacgcgctgg gcggtatccg
     8221 gcttgtcaca cagagcgaac aaggtcgggc cggagccgga gataccgctc gctaccgcgc
     8281 cgatttccgc gaccgcctgc cgcgcctgcc ggaagcctgg cagtaatcgt tcacggtagg
     8341 gttcagcgat aacatctttc atcagcttcg cggcaagctc aggctgacgg gaatagcagg
     8401 cgtgaatgaa gcctgccaga tgtcgcccgt gcgcaatgca atcctggcgg cgatactgcg
     8461 ccggtaaaat agcccgggct tctgccgtcg agactttaat ccccggatac gccagcaccc
     8521 acagccactc atcaaaccca ggcacttgct ggctgatgat gtcgttttct tcgatcatca
     8581 actgcatacc acctagaaaa cacggtgcca cgttgtcgta atgaatgctg ccggagatac
     8641 gtccttccag ctcgcccatc aaagccagca aacgagtgtc attaagcggc ttgccgcagt
     8701 gttcattcat cgccatcagc gccgcgacca ccgaacaggc gctggagcct aagcccgaac
     8761 cgatcggcat attcttttcc agagtcatcg ccactggaat ttgcttgcca agctcctggc
     8821 aaaaacgctc ccagcactga taaacgatat tttcccgtgg ttctgacggc agcttatcgg
     8881 caaagcgccc gaggttgttg agactgaata tctctgccgc ctcaaccgta actacatctc
     8941 cgagcaatgc accatcaacg ggtgtcaccg ccgccccgag cacatcaaac ccgacgctca
     9001 tattggcact ggaagccggg gcataaactt taaccatgtc agactcctaa cttccatgag
     9061 agggtacgca gcagatcggc aaagacacca gcagcggtaa cgtcattgcc cgcaccatat
     9121 ccgcgcagca ccaacggcag cggctgataa tagtggctat agaaggccag ggcgttttcg
     9181 ccatttttca ctttgaacag cggatcatta ccatccactt cggcaatctt cacgcgacag
     9241 acgccatctt catcaatatt gccaacatag cgcaaaactt ttccttcatc acgggccttc
     9301 gccacgcgcg cggcaaagcg atcgtcgagc tgtgacagat tcgccataaa agcggcaaca
     9361 tcaccctcgg cgttaaactc tgcgggcagc acaggttcaa tttcaatatc cgccagctcc
     9421 agttcacgtc ccgtttcacg ggcgagaatc aatagtttac gcgccacatc cataccagaa
     9481 agatcatctc gcggatccgg ttcggtataa cccatttccc gtgccagcgt ggtcgcctcg
     9541 gagaaactca tgccttcgtc taacttgccg aagatataag aaagcgatcc tgaaagaatg
     9601 ccggagaact tcatcaattc atcaccagca ttgagcagat tttgcaggtt ttcaataacc
     9661 ggtaatccag ccccaacgtt ggtgtcatag aggaatttcc gccgcgattt ttccgccgca
     9721 tgacgcaaca gatggtagta atccatcgac gaggtgttgg cctttttgtt cggcgtgaca
     9781 acgtggaaac cttcgcgcag gaagtcggca tattgatccg ccactgcctg gctggaggtg
     9841 cagtcaacaa tgaccgggtt cagcagatga tattctttca cgaggcgaat taagcgcccg
     9901 agattaaacg gctctttggc ttgcgccagt tcttcctgcc agttttccag atttaggcca
     9961 tgcacattgg tgagcagtgc cttcgagttg gcaacaccgc agacacgtaa gtcgatatgt
    10021 ttattcttca gccagctttg ttgacgcttc agttgctcca gcagcgcacc gccaacgcca
    10081 ccgacgccaa tcacaaacac ttcgataacc tgatcggtat tgaacagcat ctgatgagta
    10141 acgcgcacgc cagtggtcgc atcatcgtta tttaccacga cagagattga gcgttcagaa
    10201 gatccctgag caatagcgac aatgttgata ttggcgcggg ccagcgcggc aaagaatttc
    10261 gccgagatcc cacgcaaggt acgcatacca tcacctacca ccgagataat ggccagccgt
    10321 tccgtcaccg ccagcggctc cagtaagcct tctttcagtt ccaggtagaa ctcttcctgc
    10381 attgcccgtt cagcccgcac acagtcgctt tgcggaacgc agaaactgat actgtattcg
    10441 gaagatgatt gcgtaatcag caccacggaa atacgggcgc gtgacatcgc ggcaaagacg
    10501 cgcgccgcca tgccgaccat tcctttcatc cccgggccgg aaacgctgaa cattgccatg
    10561 ttattcagat tggaaatgcc cttgaccggt aattcgtctt catcacgact ggcaccaatg
    10621 agcgtacctg gtgcttgagg atttccggta tttttaatca ggcaagggat ctggaactgg
    10681 gcgatggggg taatggtgcg ggggtgaaga actttagcgc cgaagtagga aagctccatc
    10741 gcttcctggt aggacatcga cttcaacaac ctcgcatcgg gcacctgacg cgggtcgcag
    10801 gtatagaccc cgtcaacgtc cgtccaaatc tcgcaacaat cggcgcgtaa acaggcagcc
    10861 agcaccgccg cggagtagtc ggaaccgttg cgtccaagta ccaccagttc gcctttttca
    10921 ttaccggcgg tgaagcctgc catcagcacc atgtgatcag ccggaatacg acttgccgca
    10981 atacggcggg tggactcagc aatatcgacg gtagattcga ggtaatgccc cactgccagt
    11041 agtttttcga ccggatcgat aacggtaacg ttgtgaccac gcgcttctaa cacgccggcc
    11101 ataatggcga tcgacatttt ctcgccacgg caaatcagcg cagcgttaat gctatccggg
    11161 cactgcccta acaaactaat gccatgcagg acatgtttta tttgggcaaa ttcctggtcg
    11221 acgaaagttt tcaattgcgc cagcgggaat cccggctggg cggcggcgag tcccgtcaaa
    11281 agttcggcaa aaatacgttc ggcatcgctg atattgggta aagcatcctg gccgctaatg
    11341 gttttttcaa tcatcgccac caggtggttg gtgattttgg cgggggcaga gaggacggtg
    11401 gccacctgcc cctgcctggc attgctttcc agaatatcgg caacccgcag aaaacgttct
    11461 gcatttgcca ctgatgtacc gccgaacttc aacactcgca tggttgttac ctcgttacct
    11521 ttggtcgaaa aaaaaagccc gcactgtcag gtgcgggctt ttttctgtgt ttcctgtacg
    11581 cgtcagcccg caccgttacc tgtggtaatg gtgatggtgg tgataatggt ggtgctaatg
    11641 cgtttcatgg atgttgtgta ctctgtaatt tttatctgtc tgtgcgctat gcctatattg
    11701 gttaaagtat ttagtgacct aagtcaataa aattttaatt tactcacggc aggtaaccag
    11761 ttcagaagct gctgtcagag actctttttt taatccacac agagacatat tgcccattgc
    11821 agtcagaatg aaaagctgaa aaatacttac taaggcgttt tttatttggt gatatttttt
    11881 tcaatatcat gcagcaaacg gtgcaacatt gccgtgtctc gttgctctaa aagccccagg
    11941 cgttgctgta accagtcgac cagttttatg tcatctgcca ctgccagagt cgtcagcaat
    12001 gccatgactc gttcgcgtaa agcttgcagt tgatgttggt ctgccgttgt gtcacttttc
    12061 gtcggttgtt gtattaatgt tgctaattga tagcaataga ccatcactgc ctgccccaga
    12121 ttgagcgaag gataatccgc caccatcggc acaccagtaa gaacgtcagc caacgctaac
    12181 tcttcgttag tcaacccgga atcttcgcga ccaaacacca gcgcggcatg gctcatccat
    12241 gaagattttt cctctaacag cggcaccagt tcaactggcg tggcgtagta atgatatttc
    12301 gcccgactgc gcgcagtggt ggcgacagtg aaatcgacat cgtgtaacga ttcaaccagt
    12361 gtcgggaaaa ctttaatatt atcaataata tcaccagatc catgtgcgac ccagcgagtg
    12421 gctggctcca gatgtgcctg actatcgaca atccgcagtt cgctaaaccc catcgttttc
    12481 attgcccgcg ccgctgcccc aatattttct gctctggcgg gtgcgaccag aataatcgtt
    12541 atacgcatat tgccactctt cttgaccaaa tgaccgcgaa cagggtgatc actgtcaact
    12601 tattacgcgg tgcgaattta caaatcctta acgtaagtcg cagaaaaagc cctttactta
    12661 gcttaaaaaa ggctaaacta tttcctgact gtactaacgg ttgaattgtt aaaaaatgct
    12721 acatatcctt ctgtttactt aggataattt tataaaaaat aaatctcgac aattggattc
    12781 accacgttta ttagttgtat gatgcaacta gttggattat taaaataatg tgacgaaagc
    12841 tagcatttag atacgatgat ttcatcaaac tgttaacgtg ctacaattga acttgatata
    12901 tgtcaacgaa gcgtagtttt attgggtgtc cggcccctct tagcctgtta tgttgctgtt
    12961 aaaatggtta ggatgacagc cgtttttgac actgtcgggt cctgagggaa agtacccacg
    13021 accaagctaa tgatgttgtt gacgttgatg gaaagtgcat caagaacgca attacgtact
    13081 ttagtcatgt tacgccgatc atgttaattt gcagcatgca tcaggcaggt cagggacttt
    13141 tgtacttcct gtttcgattt agttggcaat ttaggtagca aacatgcaga ccccgcacat
    13201 tcttatcgtt gaagacgagt tggtaacacg caacacgttg aaaagtattt tcgaagcgga
    13261 aggctatgat gttttcgaag cgacagatgg cgcggaaatg catcagatcc tctctgaata
    13321 tgacatcaac ctggtgatca tggatatcaa tctgccgggt aagaacggtc ttctgttagc
    13381 gcgtgaactg cgcgagcagg cgaatgttgc gttgatgttc ctgactggcc gtgacaacga
    13441 agtcgataaa attctcggcc tcgaaatcgg tgcagatgac tacatcacca aaccgttcaa
    13501 cccgcgtgaa ctgacgattc gtgcacgcaa cctgctgtcc cgtaccatga atctgggtac
    13561 tgtcagcgaa gaacgtcgta gcgttgaaag ctacaagttc aatggttggg aactggacat
    13621 caacagccgt tcgttgatcg gccctgatgg cgagcagtac aagctgccgc gcagcgagtt
    13681 ccgcgccatg cttcacttct gtgaaaaccc aggcaaaatt cagtcccgtg ctgaactgct
    13741 gaagaaaatg accggccgtg agctgaaacc acacgaccgt actgtagacg tgacgatccg
    13801 ccgtattcgt aaacatttcg aatctacgcc ggatacgccg gaaatcatcg ccaccatcca
    13861 cggtgaaggt tatcgcttct gcggtgatct ggaagattaa tcggcgttac caccgtcaaa
    13921 aaaaatggcg ctttttagcg ccgtttttat ttttcaacct tatttccaga tacgtaattg
    13981 atcgtccatt gtaacttctt tactggcttt cattttcggc agtgaaaacg cataccagtc
    14041 gatattacga gtcacaaaca tcatgccggc cagcgccacc accagcacac tggttcccaa
    14101 caacagcgcg ctatcggcag agttgagcag tccccacatc acaccatcca gcaacaacag
    14161 cgcgagggta aacaacatgc tgttgcgcca gcctttcaat accgcctgca aataaatacc
    14221 gttcattaac gccccaatca gactggcgat tatccatgcc acggtaaaac cgatatgttc
    14281 agaaagcgcc agcaagagca aataaaacat caccaatgaa agccccacca gcaaatattg
    14341 cattgggtgt aaacgttgcg cggtgagcgt ttcaaaaaca aagaacgcca taaaagtcag
    14401 tgcaatcagc agaatggcgt acttagtcgc ccggtccgtt aattggtatt gatcggctgg
    14461 cgtcgtcact gcgacgctaa acgcggggaa gttttcccag ccggtatcat tgcctgaagc
    14521 aaaacgctca ccgagattat tagcaaacca gctgctttgc cattgcgcct gaaaacctga
    14581 ctcgctaact tcccgtttgg ctggcaaaaa atcacctaaa aaactgggat gcggccagtt
    14641 gctggttaag gtcatttcgc tattacgccc ggcaggcacc acagaaagat cgccggtacc
    14701 gcttaaattc agggccatat tcagcttcag gttctgcttc cgccagtccc cttcgggtaa
    14761 agggatatgc acgccctgcc cgccttgctc taacccggtg ccgggttcaa tggtcagcgc
    14821 cgttccgtta acttcaggcg ctttcaccac accaatacca cgcgcatccc cgacgctaat
    14881 cacaataaag ggcttgccta agatgatatt tggcgcgttg agctcgctaa gacgcgaaac
    14941 atcgaaatcg gcttttagcg ttaaatcact gtgccagacc tgaccggtat aaatccctat
    15001 cttgcgttct tccacgttct gattgccatc aaccatcaat gactcaggta accaaaaatg
    15061 gataaaactt cgtttccgct ccacggtttt atcctcttcc tgcaccgtat aaagctcggt
    15121 cacaggaata gcgataagcg gcccaacgag tttttgcggc ccgctggtac tttggcgaat
    15181 cgcatcttcc acatcgctac ggtaatcggc acgttcgaca atcacttgcc gaatcagcat
    15241 tatcggaatc aacaacagca atactgcacc aaacaaggtg gtcattttcc agaacagggg
    15301 ggatttcaac atagtcttct cctttgcaat ggcagcagga tacgaaggct atgtgggaag
    15361 aatttgaagc tatgtgaagt gacggtgaag tcgaagcgag gccagcacgc caccttcctg
    15421 cacgttgcgc agcgtgactt cgccgttaaa caaacgggcg acctcactga cgaacgccaa
    15481 ccccagaccg ctgctttttt gcccatttgc acgcggcaaa gagtaaaagc gttcaaaaat
    15541 acgtgacagc gcgtagtcag gaataccact accggtatcc agcaccttaa gggcgacgtg
    15601 ttcctgatcc acttcggcgc ttagcgttat gcgaccgctc tcgggggtaa aatcgatggc
    15661 gttatccagt aaattcccca gcgcctgctc cagtaacgcc ggttcagcag caacattaac
    15721 ctcagtgggc ataacatgta gagtgatgtt tttttctgcc aactgcacgg tgcgcgcttc
    15781 gctgacgcgg cgaaataatg ccgccacatc aacaacagtc agaacgactt cctgacgatt
    15841 ctccagtctt gcctggcgta gtaacgtttc taccaatgcc tgcatacgcg cattttgcgt
    15901 cagaatgttg tcggtaaaac gagccaccac ttccggtggc ggaccttcgc gtaaaatttc
    15961 cgccgcgcca cgaatcgccg ccagtgggct ttttagctca tgggttaacg cataaacata
    16021 ctgctcaata tagtttttcc cttccagctt cacgcgcata ctttccagcg cctgcgcaag
    16081 cttacgcaac tcgctactgc cgagttcagg aagaggaacg ggcttattgt cagtgacgga
    16141 atcggcatag cgagtgagcc gggcaatgga gcggttgatc caccaaacca tgcctgcgcc
    16201 aatcaccagt gcaatcccca gcaaaatggc gctggcccat aaaattcgcc gctcgctacg
    16261 cttaatgacc ggagccatcg ccgcgttcgg tttgcctacg ctcaaaacgc caataagccg
    16321 cgagccgtcc ataatcggcg cggcaacata catcaccgaa ctttcgggat cggcaggatt
    16381 ttgcaacgtg ctgcgcgcac catactgacc acgcaacgtt agccagacgt cattccagcg
    16441 cgaataatcc tgcccaacgg ctttatttgc cgaatcgaac aatactttac cctgcgaatc
    16501 ggtcatatag acatgatatt cattgcgcac tttgttaatg ccaccgatat tggcgcgaaa
    16561 cgggcgatgt tgtagctgat taaacgcctg cgccagttgc ccatgcgttg ggtccccaga
    16621 gagcaaatcg ggacgcgcca gctccgccag caacgttgcg gtatcgatta acgtcccctc
    16681 cgttgctctt cgcacgcccg gtttaacttc tttgacaaaa atagccagta cgaaccaggc
    16741 cgccaccgcc accagtaaaa aatagcccag cagcaaccgc atgccgatac gcattacagg
    16801 cccctcaggc tatatcccat gccgcgatga gtattaatcg gtgaaagatc ggggttgatg
    16861 gcgcgcagct tggcacgcaa cgttttaatg tgggtatcaa tggtgcgatc gtaggtgtcc
    16921 tgcgcatctt cccacacgct atccattagt tgctggcggg accagacgcg gcctggcgac
    16981 ttgagtaacg tcttcagcaa taaaaactca taccgcgtca gcgttaatgg cgtgtcaaac
    17041 cagctgatct gcgccgcggg ttcattcaat tcaaaatgac caatacggat gacgggagac
    17101 ggcgacgaga acttcttcac ccgacgcagt aaggtgcgca cccttgcgca cacttcgcgg
    17161 ggtgaaaacg gtttagccac atagtcgtca gcgccaattt ccagcccaag caggcgatcg
    17221 acctcttcac ttcgggccgt caggaacagt acaggtaacg ccggatggag cgccagtaac
    17281 tggcggcata attcaaagcc gctgatatca ggcaaaccaa catcgagaat catgacgtcg
    17341 ggagcctgct ggcgagcttt atccagcacc ggcaagcctc gctcaaagac ctcgacggca
    17401 aaaccttcct gctgcaacat gtagaccagc gtgtcggcta tcccttgctc atcttccact
    17461 aaccagaccg tttcccgttg cataaatcgc ctctgttatt gccgccacgg catgacagga
    17521 accgcgctaa tcgcgttttt cggcgaacct tctacaactt tgtcggagta agccagataa
    17581 gcgagcgcgt tgcgtttggc gtcataaaag cgcacgacct gtaacgactt aaagaccagg
    17641 gacgtgcgtt ttttgaatac tacctcgccc tgagctttgc cgtttttaat acgatccgac
    17701 agttcaatcg gcccgacttg ctgacaagaa atagccgcat cggaggtatc ttccgccaga
    17761 cccaatcccc ctttaatacc cccggttttc gcccggctca cataacaggt gacatttttc
    17821 acatcgggat cgtcaaaggc ttccacaaca attttgtgat ccgggccgat cattttaaat
    17881 acggtgtcga ccgaaccaat ctcctctgca tgagccaatg gccccagcat tatcaggctt
    17941 aaagacagga tcaaatgctt gtatttcata ttgttaccat ttttatctat tacacgagca
    18001 attagttcgt cacggtaaat aacatattga ttaagaacaa aatctcaata cttttatttc
    18061 cgtcaagccc taaaacatac tctactaaga aaaaaacact gaatgctaaa acagcaaaaa
    18121 atgctattat ccaattacct gatgtcaggt gctcgttgtt gaaaggatga ggatatttta
    18181 tggatcaggc cggcattatt cgcgaccttt taatctggct ggaaggtcat ctggatcagc
    18241 ccctgtcgct cgacaatgta gcggcgaaag caggttattc caagtggcac ttacagagaa
    18301 tgtttaaaga tgtcactggc catgctattg gcgcgtatat tcgtgctcgt cgtttgtcga
    18361 aatcggcggt cgcactacgc ctgactgcgc gtccgattct ggacatcgcg ctgcaatacc
    18421 gcttcgactc tcaacagaca tttacccgcg cattcaagaa gcagtttgcc cagactcctg
    18481 cactttaccg ccgttctcct gaatggagcg cctttggtat tcgcccgccg ctacgtctgg
    18541 gtgaattcac tatgccagag cacaaatttg tcaccctgga agatacgccg ctgattggtg
    18601 ttacccagag ctactcctgt tcgctggagc aaatctctga tttccgccat gaaatgcgtt
    18661 atcagttctg gcacgatttt ctcggcaacg cgccgaccat tccgccggtg ctctacggcc
    18721 tgaatgaaac gcgtccgagt caggataaag acgacgaaca agaagtattc tataccaccg
    18781 cgttagccca ggatcaggca gatggctatg tactgacggg gcatccggtg atgctgcagg
    18841 gcggcgaata tgtgatgttt acctatgaag gtctgggaac cggcgtgcag gagtttatcc
    18901 tgacggtata tggaacatgc atgccaatgc ttaacctgac gcgccgtaaa ggtcaggata
    18961 ttgagcgata ctacccggca gaagatgcca aagcaggaga tcgcccaatt aatctgcgct
    19021 gtgaactgct gattccgatc cgtcgttaac gctgcaactc atctaatgca ggggcgtcca
    19081 gatgcgagat gtcccctgcg gtttcaacaa cccagccaga cgccagccac aggctttcct
    19141 gataatccac gcgcgaaata gaacagttac gcagacgtaa gcggcgctct gcccatgctg
    19201 gtaatccgag aatcgtactc accaggcatc ccagtgcaat accatgactc accagcagcg
    19261 gtcggcttcc ctgcggtaag tcccggcaag attccagtgc cgcattgacg cgatcgctga
    19321 gctcctgcat tgactcgcct tcaggaatac gcccgtcaac ggtgccattg accagctgcc
    19381 gacgccagtt ctcttcttct tcggtcagag aatcgatatg tcttttttcc agcacaccca
    19441 tgtttaattc acgcaggcga gaatcaaaga tgatgtcgca gccgcaggcc tgggcgatga
    19501 tttccgccgt acgccgggtg cgtcctaaat cgctactgat gatatgcgta atgccaagct
    19561 ctttggcacg ggttgccacc tgcatcgcct gttgctcacc tttggcggtc agcgggctgt
    19621 cagactggcc ctgaatacgt cgctcggcgt tccactgcgt ttcaccgtgg cggactaggt
    19681 atacctgtaa catgcttttt ttccgtatac tgctgtaacg ttaattttct gagtaatgct
    19741 gattatgcac caagttgtct gtgcgaccac caatcccgct aaaattcagg ccattctgca
    19801 ggcatttcac gagatcttcg gcgaaggatc ctgccatatt gcatccgttg ccgtcgagag
    19861 cggtgtaccg gaacaaccct ttggcagtga ggaaacgcgc gctggcgcac gaaatcgggt
    19921 agcaaatgcc cgccgtttac ttccagaggc tgatttttgg gtggcgattg aagctggcat
    19981 cgatggtgac agcactttca gttgggtggt gattgaaaac gccagccagc gcggcgaggc
    20041 gcgttcggct actttgccgt tgcctgcggt gattctggaa aaagtgcgtg aaggcgaggc
    20101 gctcggcccc gtgatgtcgc gttataccgg tattgatgag attggtcgta aggaaggggc
    20161 gatcggcgtg tttaccgccg ggaaactcac ccgcgccagc gtctatcacc aggcggtgat
    20221 cctggcgctg agtccgtttc ataatgccgt gtatcaggca ttgtaggacg gataaggcgt
    20281 tcacgccgca tccggcattc ggtgcac
//
LOCUS       DAZGLJ010000069        20228 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000069 DAZGLJ010000000
VERSION     DAZGLJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.069"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            218..1084
                     /gene="folD"
                     /locus_tag="ACHK49_002210"
     CDS             218..1084
                     /gene="folD"
                     /locus_tag="ACHK49_002210"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO9262855.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            1086..1298
                     /gene="ybcJ"
                     /locus_tag="ACHK49_002211"
     CDS             1086..1298
                     /gene="ybcJ"
                     /locus_tag="ACHK49_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO9262856.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVEVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1406..1927
                     /locus_tag="ACHK49_002212"
     CDS             1406..1927
                     /locus_tag="ACHK49_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO9262857.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHREFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(1963..3348)
                     /gene="cysS"
                     /locus_tag="ACHK49_002213"
     CDS             complement(1963..3348)
                     /gene="cysS"
                     /locus_tag="ACHK49_002213"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO9262858.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYIALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3522..4016
                     /gene="ppiB"
                     /locus_tag="ACHK49_002214"
     CDS             3522..4016
                     /gene="ppiB"
                     /locus_tag="ACHK49_002214"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO9262859.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4019..4741
                     /gene="lpxH"
                     /locus_tag="ACHK49_002215"
     CDS             4019..4741
                     /gene="lpxH"
                     /locus_tag="ACHK49_002215"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO9262860.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4859..5368
                     /gene="purE"
                     /locus_tag="ACHK49_002216"
     CDS             4859..5368
                     /gene="purE"
                     /locus_tag="ACHK49_002216"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO9262861.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5365..6432
                     /gene="purK"
                     /locus_tag="ACHK49_002217"
     CDS             5365..6432
                     /gene="purK"
                     /locus_tag="ACHK49_002217"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO9262862.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(6569..7462)
                     /locus_tag="ACHK49_002218"
     CDS             complement(6569..7462)
                     /locus_tag="ACHK49_002218"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO9262863.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVDPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQKALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFTKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7459..8274)
                     /locus_tag="ACHK49_002219"
     CDS             complement(7459..8274)
                     /locus_tag="ACHK49_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO9262864.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8285..9544)
                     /locus_tag="ACHK49_002220"
     CDS             complement(8285..9544)
                     /locus_tag="ACHK49_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO9262865.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9554..11221)
                     /locus_tag="ACHK49_002221"
     CDS             complement(9554..11221)
                     /locus_tag="ACHK49_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO9262866.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELTNQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSSATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11537..12586
                     /gene="allD"
                     /locus_tag="ACHK49_002222"
     CDS             11537..12586
                     /gene="allD"
                     /locus_tag="ACHK49_002222"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO9262867.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFFSS
                     KLFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12608..13843
                     /gene="allC"
                     /locus_tag="ACHK49_002223"
     CDS             12608..13843
                     /gene="allC"
                     /locus_tag="ACHK49_002223"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO9262868.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            13854..14639
                     /gene="allE"
                     /locus_tag="ACHK49_002224"
     CDS             13854..14639
                     /gene="allE"
                     /locus_tag="ACHK49_002224"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO9262869.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14768..15913)
                     /gene="glxK"
                     /locus_tag="ACHK49_002225"
     CDS             complement(14768..15913)
                     /gene="glxK"
                     /locus_tag="ACHK49_002225"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO9262870.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGETVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(15935..17235)
                     /locus_tag="ACHK49_002226"
                     /pseudo
     CDS             complement(15935..17235)
                     /locus_tag="ACHK49_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
     gene            complement(17292..18653)
                     /gene="allB"
                     /locus_tag="ACHK49_002227"
     CDS             complement(17292..18653)
                     /gene="allB"
                     /locus_tag="ACHK49_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO9262871.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(18713..20167)
                     /locus_tag="ACHK49_002228"
     CDS             complement(18713..20167)
                     /locus_tag="ACHK49_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO9262872.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLIFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENGGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIHFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
BASE COUNT         4904 a         5557 c         5153 g         4614 t
ORIGIN      
        1 tccttaaaca aggaaataaa tcagttccag atttacaacg ccatcatgga cgaaaaatga
       61 agctttcaat ctcagcgacc tcagcgacgg tgcgcctcac cttcgcaaga ggtcgcttca
      121 cgcgataaat ctgaaacgaa acctgacagc gcgccccgct tctgacaaaa taggcgcatc
      181 cccttcgacc tacgtaacag atggaatcct ctctctgatg gcagcaaaga ttattgacgg
      241 taaaacgatt gcgcagcagg tgcgctctga agttgctcaa aaagttcagg cgcgtattgc
      301 agccggactg cgggcaccag gactggccgt tgtgctggtg ggtagtaacc ctgcatcgca
      361 aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc gggttcgtct cccgctctta
      421 tgacctcccg gaaaccacca gcgaagcgga gctgttggag cttatcgatg cgctgaatgc
      481 cgacaacacc atcgatggca ttctggttca actgccgtta ccggcgggta ttgataacgt
      541 caaagtgctg gaacgtattc atccggacaa agacgtggac ggtttccatc cttataacgt
      601 cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc accccgcgcg gtatcgtcac
      661 gctgcttgag cgttacaaca ttgatacctt cggcctcaac gccgtggtga ttggcgcatc
      721 gaatatcgtc ggtcgtccaa tgagcatgga actgttgctc gcaggttgca ccactaccgt
      781 gactcaccgt ttcactaaaa atctgcgtca ccacgtagaa aatgccgatc tgttgatcgt
      841 tgccgtcggc aagccgggct ttattcctgg tgactggatc aaagaaggcg caattgtgat
      901 tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg ggcgacgtcg tgtttgaaga
      961 cgcggctaaa cgcgcctcat acattacgcc tgttccgggc ggcgttggcc cgatgacggt
     1021 tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa tatcatgatc cacaggatga
     1081 gtaacatggc gacattttct ttaggtaaac atccgcacgt tgagctgtgc gacttgctga
     1141 aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat cgcgattgcc gaaggccagg
     1201 tggaagtcga cggtgcggtt gaaacgcgca aacgctgcaa aatcgtcgcc ggtcagacag
     1261 tgagttttgc aggtcacagc gtacaggttg ttgcctgatc cctttcccgg cctgtatcaa
     1321 caggccggtt ctcttttcag acttttgccc tctacgcccc gttcatccat gctgatagca
     1381 tcgacatttt gccgaagcga ccatcatgcc aaccgttatt acgcacgccg ccgttcccct
     1441 atgcattggt ttagggttgg gttcgaaagt catccctccg cgtctgttat ttgccggaat
     1501 catcctggcg atgctgcccg acgccgacgt attgtcgttt aaatttggcg ttgcttacgg
     1561 caatgttttt ggtcatcgcg agtttaccca ttcgctggtg tttgcgtttg ttgtcccgct
     1621 gttatgtgtg ctgattggac gccgatggtt cagggctggg ctgattcgct gctggctgtt
     1681 tttaaccgtc tcgttgctat cgcacagctt gctggattcg gtaaccactg gcggtaaggg
     1741 cgttggctgg ctgtggccgt ggtcagatga acgctttttc gctccctggc aggtgattaa
     1801 agtcgcgccg tttgcattgt ctcgttacac cacgccgtac gggcatcagg tgattatttc
     1861 tgaattgatg tgggtgtggt tgccggggat ggtgcttatg ggaatgttgt ggtggcgcag
     1921 gcgataaccg gatgcgaaaa ctcgcatccg gcaacagcgc aattacttac gacgccaggt
     1981 ggtcccttgc gggccatctt ccagcacgat ccccatctcg ttaagacggt cacgcgccgc
     2041 atctgccgcc gcccagtctt tcgctttacg ggcatccaga cgctgttgaa ttaacgcttc
     2101 aatctcagcc acttcgctgt cgtctgcctg cgcaccgctt tgcaggaacg cttccggttc
     2161 ttgctccagc aggcccaata cagcggaaag tttacgcagg tgagatgcca ttgcattcgc
     2221 cgctgccata tcttctgctt tcagacggtt cacttcacgc gccatatcaa acagcacgga
     2281 ataggcttcc ggggtgttga aatcgtcgtc catcgcttca ataaagcgcg cttcaaacgc
     2341 ttcgccaccg gcaggcgcaa cggttttatc tgtgccgcgc agcgcaatgt agaggcgctc
     2401 cagcgccgca cgcgcctgct tcaggttctc ttcgctgtag ttcagttggc tgcgatagtg
     2461 gcccgacatc aggaagtaac gcacggtttc cgcgtcgtag tatttcagca catcgcgcac
     2521 ggtaaagaag ttacccagcg atttggacat cttctcgcgg tcaaccatca ccatccccga
     2581 gtgcatccag tagttcacat actggccatc atgggcacag gtggactgcg cgatttcgtt
     2641 ctcgtggtgc gggaacatca ggtctgaacc gccgccgtgg atatcaaagt ggttacccag
     2701 ctgcttgcag ttcattgccg aacattcaat gtgccagcct ggacggcccg cgccccacgg
     2761 agacggccag ctcggttcgc cctctttcga catcttccac agaacgaagt ccatcgggtt
     2821 gcgtttgtcg tcgaccacgt caacgcgcgc acctgcctgc agctggtcga gatcctgacg
     2881 cgacagcacg ccatatgtcg gatcggtcgg gacgtcgaac atcacgtcgc cgttgtccgc
     2941 cacataagcg tgacctttgg cgatcagttg ttcagtgagt tcaataattt ctgcgatatg
     3001 gtgcgtcgcg cgcggctcca tatccgggcg cagaatgttc aaagcatcaa aatctttgtg
     3061 catttcggcg atcatgcgat ccaccagcgc cacaaagctt tcgccatttt cattggcgcg
     3121 tttgatgatt ttgtcgtcga tatcggtaat gttgcgcaca tacttcagtt tatagccgag
     3181 gaaacgcaga tagcgcgcaa ccacgtcaaa agcaacaaag gtacgcccgt gaccgatatg
     3241 acagagatcg taaacggtga ttccacacac atacatgccg acttccccgg cgtgaatagg
     3301 cttaaattcc tctttttggc gtgtcagagt attgaagatt tttagcatcg aagattccgt
     3361 ttagacatgt gtgggtaatt gagttgcgta taatacccat atttcccgcg cgaatcagca
     3421 tacattgcgt gatgatccga tcgtgcggtt atgctataac accaccctat atatgacccg
     3481 aactgggttg aagcaccaat caaacggaac aggatgcaaa aatggttact ttccacacca
     3541 atcacggcga tattgtcatc aaaacttttg acgataaagc acctgaaaca gttaaaaact
     3601 tcctggacta ctgccgcgaa ggtttttaca acaacaccat tttccaccgt gttatcaacg
     3661 gctttatgat tcagggcggc ggttttgaac cgggcatgaa acaaaaagcc accaaagaac
     3721 cgatcaaaaa cgaagccaac aacggtctga aaaatacccg tggtacgctg gcaatggccc
     3781 gtactcaggc tccgcactct gcaactgcac agttcttcat caacgtggtt gataacgact
     3841 tcctgaactt ctctggcgaa agcctgcaag gttggggcta ctgcgtgttt gctgaagtgg
     3901 ttgaaggcat ggacgtggta gacaaaatca aaggtgttgc aaccggtcgt agcggtatgc
     3961 accaggacgt gccaaaagaa gacgttatca ttgaaagcgt gaccgttagc gagtaatcgt
     4021 ggcgacactc tttattgcag atcttcatct ctgcgtggaa gaaccggcga tcaccgccgg
     4081 ttttctgcgt tttttagcgg gggaagcccg caaggccgac gcgctgtata ttcttggcga
     4141 tctgtttgaa gcatggattg gcgacgacga gcccaaccca ctccatcgcc agatggcggc
     4201 agcgatcaaa gcggtgtccg attccggcgt tccctgttat ttcattcatg gcaaccgtga
     4261 ttttctgctc ggcaaacgct ttgcccgtga aagcggcatg acattattgc cggaagaaaa
     4321 ggtgctcgaa ctttatggtc gccgggtgtt gattatgcat ggcgacacgc tgtgcaccga
     4381 tgacgcgggt tatcaggctt ttcgcgccaa agtccacaaa ccctggctgc aaatgctatt
     4441 cctcgccctg ccgttgtttg tgcgcaaacg cattgccgcg cgaatgcgag cgaacagtaa
     4501 agaagccaac agcagtaaat cgctggcgat catggacgtt aaccaaaacg cggtagtcag
     4561 tgcgatggaa aaacatcagg tgcaatggct gatccacgga catacccatc gcccggcggt
     4621 acatgaactt atcgccaacc agcaacctgc ttttcgcgtg gtactcggtg cctggcatac
     4681 ggaaggttcg atggtgaaag tcacggcgga tgacgttgag ctgattcatt ttccgtttta
     4741 aaaaacccgc aactttgctg atttcacagc cacgcaaccg ttttccttgc tctctttccg
     4801 tgctattctc tgtgccctct aaagccgaga gttgtgcacc acaggagttt taagacgcat
     4861 gtcttcccgc aataatccgg cgcgtgtcgc catcgtgatg gggtccaaaa gcgactgggc
     4921 taccatgcag ttcgccgccg aaatcttcga aatcctgaat gtcccgcacc acgttgaagt
     4981 ggtttctgct caccgcaccc ccgataagct gttcagcttc gccgaaagcg ccgaagagaa
     5041 cggttatcag gtgattattg cgggcgcagg cggcgcagcg cacctgcctg gtatgattgc
     5101 cgccaaaacg ctggtgccag tgctgggcgt gccagtacag agcgccgcac tgagcggtgt
     5161 cgatagcctc tactccatcg tacaaatgcc gcgcggcatt ccggtgggta cgctggcgat
     5221 tggtaaagct ggcgcggcaa acgcggcgtt actggcagca caaattcttg cgactcatga
     5281 taaagaactg caccagcgtc tgaatgactg gcgcaaagcc cagaccgacg aagtgctgga
     5341 aaacccggac ccgcgaggtg cggcatgaaa caggtttgcg tcctcggtaa cgggcagtta
     5401 ggccgtatgc tgcgtcaggc aggtgaaccg ttaggcattg ctgtctggcc ggtcgggctg
     5461 gacgctgaac cggcggcggt gccttttcaa caaagcgtga ttaccgctga gatcgaacgc
     5521 tggccggaaa ccgcattaac ccgcgagctg gcgcgtcatc cggcctttgt gaaccgcgat
     5581 gtgttcccga ttattgccga ccgtctgact cagaagcagc ttttcgataa gctccacctg
     5641 ccgaccgcac cgtggcagtt acttgccgat cgcagcgagt ggcctgcggt gtttgagcgt
     5701 ttaggtgaac tggcgattgt taagcgtcgc actggtggct atgacggtcg cggtcaatgg
     5761 cgtttacgtg ccaatgaaac cgaacagtta ccggcagagt gttacggcga atgtattgtc
     5821 gagcagggca ttaacttctc tggtgaagtg tcgctggttg gcgcacgcgg atttgatggc
     5881 agcaccgtgt tttatccgct gacgcataac ctgcatcagg acggtatttt gcgcaccagc
     5941 gtcgcttttc cgcaggccaa tgcgcagcag caagcgcaag ccgaagagat gctgtcggcg
     6001 attatgcagg agctgggcta tgtgggcgtg atggcgatgg agtgttttgt caccccgcaa
     6061 ggtctgctga tcaacgaact ggcaccgcgt gtgcataaca gcggtcactg gacacaaaac
     6121 ggtgccagca tcagccagtt tgagctgcat ctgcgggcga ttaccgatct gccgttaccg
     6181 caaccggtag tgaatagtcc gtcggtgatg atcaacctga ttggtagcga tgtgaattat
     6241 gactggctga aactgccgct ggtgcatctg cactggtacg acaaagaagt ccgtccgggg
     6301 cgtaaagtgg ggcatctgaa tttgaccgac agcgacacat cgcgtctgac cgcgacgctg
     6361 gaagccttga tcccgctgct gccgccggag tatgccagcg gcgtgatgtg ggcgcagagt
     6421 aagttcagtt aagcaggtgc tcatttgatt gccggatgcg gcgtaaacgc cttatccggc
     6481 ttaccgctcc ggtacccatt gtaggcctga taagacgcgt caagcgtcgc atcaggcatg
     6541 gtgcaccgat tgccggatgc tctcatcact acagcgaaat acaggtcccc gcttcgcccg
     6601 ccagcgtctc ttcaattcgc gataacgccc caatccacgc gggtttacca cggcttctga
     6661 cataaccact taccgccgtt accttcggcc ccatcgaacc atcggctttg gtaaatggcg
     6721 ctaactcatc cggtgtggca tggcgaatgg cgcgttgctg cggcgttccc cagttttcat
     6781 aaaccgcgtc agcatcggtg aggatcacca gtccatcggc attaatctgc tcggcgagca
     6841 acgcagcggc gagatcttta tcaatcaccg cttcactccc tgccccatct tccgtcacag
     6901 gcacaccgcc gccgccactg caaatcacca catgcccctc tttgagcaac aactcgatgg
     6961 cttcgctgtc gagaatttta cgcggttgcg gagacgccac cacccggcgc aaatatttac
     7021 catcacgttt catctgccag ccgtaagccg cttccagtgc cttttgttct tctggctgat
     7081 aaaccggacc aataaatttc tctggctgca aaaacgccgg atcatcaggc gaaacctcaa
     7141 tgcgcgtcag caccgtcgtg acaggcggca tctgcggctg tgcgctcaaa ctctgcgcca
     7201 gcatatagcc aatcatcccc tggctttccg ccaccagcac atccagcgga tacggatcta
     7261 cctctttcca cgccagattc tgcaatgcca gcagccccac ctgcggccca ttgccgtgaa
     7321 caatcgccag ccgataagaa cgggccaggc gcgccagcgc gggtacagca ctggcgatat
     7381 tgcgatattg attttctgcc gtcagcgcct caccgcgctg gagtaaggcg ttgcccccaa
     7441 gagctacaac cagtgttttc atggttttcc ttgtaataat tgttggccaa gccagaaccc
     7501 cagcagcgta tctgcccctg acgtatgccc cagcgccagc agcgaatcaa tcgcaacagc
     7561 ggtacgcttc gggcaactca gagcatggac aaagtgcagg agtggcgagg cgaaatatcc
     7621 ttgcgcggca taacgtaaat aactgacgct caccgctgtg gtaacgagtt gaagattgtc
     7681 ggaacaggca aaaaacgggc gaccggcgcg cgcatctaaa gcgccataat accaggccgc
     7741 cagcagcatt ccgctcaacg tgtcatcatg acttggcgtt aatccggggc ctttacccag
     7801 ccagtgccgc cagtcggtct taacgccatt gagcgcggcc tgaaaacagt gacgaaactg
     7861 gcgtaattca gcgggcagcg gatcgcttgc cgccatcgcc agtggtccga aaagtccggt
     7921 ttcctccgcg cgttgcatcc atgcagctgc aagcggttga ggatgcgaag gcggcgtaat
     7981 acgcagcaaa caataacgct gtggctgttt aaccgtgaaa cgcccgaggc gaatcccttg
     8041 agccacaacc tgtggtcgtt cattgccgca taatccaccg cataacgcat cgaattgcgc
     8101 gcggcgaagc acccatcctc cggggccgaa accactaccc tgacgatgca gcgttaacag
     8161 ttcgccgctt tccgtcatca ggttaatcgc ccgtcgccac actcccgcta accgccagga
     8221 ctggcgataa ttcggtgcac tacggctggc aagcagagga tggatgatcg tcattgcgcg
     8281 ttcctcaacc aatccccatg ctttcagcca gtgcttccag cgcctgttca aaacacgcca
     8341 gcggtgcccg cacagtgcct gcgccaatct gcccgatccc cgcctcttta tgggcgatac
     8401 cggtattgat gagtggcgta atgccggttt ctaccacgcg acgaatatcc agccccaggc
     8461 acgcgccctg gaaatcccag ccggggatct gcaactgcat attgcgctca aggtaaattt
     8521 ccgccatctc ttcagatacc gctctggccg cttccatgcc acccgcaccg acaaagcgcg
     8581 ttacgccagg cgctgcgatc attgccgcac ctccgatacc aaaggtttcg gtaatcgcgc
     8641 tatcgcccat atccgggttc gcctgctcct gcgagaagcc ggtgaaaaac agaccttgcg
     8701 gagtgtttac aggcgcagta aaccagcgtt cgcccagccc gcttacccga atcccgaaca
     8761 tattgccgtt gcgggtcatt gccgtgacga tgctgcctgc gcggatcatc gcgccagcat
     8821 ccatcgccgc cttgcagtaa gccatcgcga ggttgaggaa gaactgatcg gtcacgctga
     8881 ggaaatccat cacttcggcg atgtgctgtt tatcatgatc gaggcgagca atttgtgggg
     8941 ccagcgcacg cattaacagt gcagaggaag caatattgcg ttgatggaac tcatcgccca
     9001 tcgtaatgcc ctgcgccatc atcgccgtga gatcgatacc gcgctccatg cgccccagcg
     9061 ccgcgcttaa tactggcatt aacacatcgc gcatccagcg atggcgagtc aggacatctt
     9121 cgccgtaagc gccaaaacgc atcactttgc cgataccttc gttgaggttg cagtacgccc
     9181 ggttgccgtc ggtcacgttc tcgaccacca gcatcggcat actggcagaa gtaataccgc
     9241 ccattggacc gacggcattc acatggtgac aaggaatgaa gttcacttcc ccctgctcca
     9301 gtattgccag cgcctgcgct tcatctttcg cccagccttc gaacagacat gcgcccacgc
     9361 acgccccttt catgggtccg gtcatctcct gccagcgcat tggcggcccg gcgtgaagca
     9421 gtgttttgcc ctcgtttagt tcgctgataa gtgaagaagc cggttgcaca tccagccagt
     9481 gtggacgagc acgacgaatt tgttcgataa ccgcagcatt ggcttgcgcc actgatgtaa
     9541 acatgggaac cccttattgc aaacgttcta ataaacgagc cagtttttta ttgccgccgg
     9601 cgactggcga ccattggtaa tgcacaaccg gtttgctggc gctttgtagc tccagcgcaa
     9661 agctgcgtaa tccgatgtta atcacggcga cgttttccag taatgacggt gtgtgttgct
     9721 gtgtggcaga cgagagcgga tgaattaacg ctgccgccag caaggtggct tccggtagcg
     9781 aactcactac cgcgatcccc gcatcttcca gcgtggcgat ttgctgcgag cggcattgcg
     9841 ggtcacgttc ggtacctgtc accgtggcaa tggcgtacag cggttggctg tctgaacgcg
     9901 cggcacaggc tttttgccag gcgctcacca gcgaggcagc aggatcggcg gtcgcaccga
     9961 agccaatcac gacatcaagc agcaacacgc gcacttgcgg tttagcgccg agatcggcaa
    10021 ttaactggtt gcgtaaggct gggtcgatca tcggatgggg acgcccgacg gtgtagaaat
    10081 catcgcccag atcaatgatc tggtgaccat cggcgtccag catcatgccg tgatgatggg
    10141 tatcgtcggc ttccacgcca aggtgcccgg caagtaatcc cgccgcttcg gcagcaagcg
    10201 taccgccggt atacaaaccg caaataaatc cgctgctgac aggcgctatt gcgttacgtc
    10261 gcgccgtgac gcgtgaaagc aggcaagcca ggcgtgcggc ctcatccagc gaggaggcaa
    10321 accagacatt ctcgtcgcgg gccaccgccg gggtataacc taaaaacagc gccaccgtcg
    10381 gtttgccggt tgctttcatg gcattaacaa ttttcagacg cacagcttcg gcaggtggtt
    10441 ttgaaacaaa tgccagcact tcgcttttct cgtctgcact gagcatttcc agcgctgtta
    10501 gcgcactgat gccgcccact tcacggctga ggtcgcgccc gccaaggcca atcgcgtgag
    10561 taattccctc ccctgccagc gcaatctgcg aacacagctc ctgaatcccg gtaccggaag
    10621 cgccaatgac gccaatattg ccttctggca tcacgttagc aaaagccagc ggtgtgccgg
    10681 caatcatcga cgtaccgcag tccggcccca tcaccagcaa gcctttttcc cgcgcgcggg
    10741 ttttaagttg gatttcatct tccagcgtga cgttatcgga gaacatcatc acgttgaggt
    10801 tgcgatccag cgcctggttt gtcagctccg ccgcatactc gccagccact gaaatcagcg
    10861 ccagattggc atcgggtaat ttctgacagg cgctgtccca gcgacgcacc tgcgtcaacg
    10921 cctggctgct gcctgacccc tgcgccagtt gttttagcgc ctcttcaagc tgctgcataa
    10981 tcgcctgcgc gatccccgca tccgccgctt cgctacgaat tgccacgcaa atatcgttcg
    11041 gcgtggcgtt attaaaatcg tcatgccaga aacctgtggt atctaataac gctttattgg
    11101 cgggcgtacc catcattacg gaaacatcat caacattttc tgattcgctg agttttcgtg
    11161 aaataatcat taaactgacc gaatcctgaa aacacccttt tttaataaag gcgtggatca
    11221 taacaactcc ttaaaaattg cacccatggc aattgcacga gcagtgttaa tgtggcgatt
    11281 acggtatcga tgacaacgag taaataaggt gatgaatatc acacagggat tattaacaaa
    11341 agaattcaaa taaccgccag gctattacgc attatttttt aataagcgtg gttgttttaa
    11401 aaatcaggtt aaaagcgcga agattatcaa ataaattcta actatatttt ttgcctgtct
    11461 ggatcacata atccagatat tttccctgta tgttaattgc agtcatgctt cacaccgtcg
    11521 ttaaaaagga agacagatga aaatcagtcg ggaaacactc caccagctaa ttgagaataa
    11581 actctgccag gctgggttaa aacgtgagca cgctgcaacc gtggctgaag tattggttta
    11641 cgccgatgcc agagggatcc actctcatgg cgcggtgcgc gtggaatact acgcggaacg
    11701 catttcaaaa ggcggcacca accgcgaacc ggagtttcgt cttgaggaaa ccgggccgtg
    11761 ctcggcaatt ttacatgccg acaatgccgc cggacaggtc gcggcgaaaa tgggtatgga
    11821 acatgccatc aaaaccgccc agcaaaatgg cgttgcggtg gtcggtatca gccggatggg
    11881 tcacagcggc gcaatctctt attttgtgca gcaggcagcc cgcgccggat taattggtat
    11941 ttcgatgtgc cagtccgatc caatggtggt gccgtttggc ggcgcggaaa tttactacgg
    12001 tactaaccca ctggcctttg ccgcgccggg agaaggcgac gagatcctta cctttgatat
    12061 ggcgactacc gtacaggcat ggggaaaagt gctcgacgcc cgctcgcgta atatgtctat
    12121 cccggatacc tgggcggtcg ataaaaacgg tgcaccaaca accgatccgt tcgcggtaca
    12181 tgctctgctc cccgccgccg ggccaaaagg gtatggcctg atgatgatga ttgacgtcct
    12241 ctcaggcgtc ttactcggct taccgttcgg gcgacaggtt agttcgatgt atgacgattt
    12301 acacgccggg cgtaatttgg ggcaattaca tatcgttatt aacccgaact ttttcttctc
    12361 cagcaaatta tttcgtcaac atcttagcca gaccatgcgc gaattaaatg ccattacccc
    12421 cgcgcccggt tttaatcagg tttattatcc cggacaggat caggatatta aacaacgcaa
    12481 agccgccgtc gaaggcatcg aaattgttga tgatatttac cagtatttaa tttccgacgc
    12541 gctttataac acgtcatacg aaacgaaaaa tccctttgcg caataattat tgagacagga
    12601 ctttcttatg attacacatt ttcgtcaagc catagaagaa acgctgccct ggctttcctc
    12661 ttttggcgct gacccaacgg gtggtatgac ccgtttactt tattcgccgg aatggctgga
    12721 aactcagcag caatttaaaa aaagaatggc agcaagcggg ctggaaacac gtttcgatga
    12781 agtggggaat ttatatggtc gcctgagtgg caccgaatat ccacaagaag tggttctgag
    12841 cggttcgcat atcgataccg tggttaacgg cggtaacctc gacgggcaat tcggcgcgct
    12901 ggcggcgtgg ctggcaattg actggctgaa aacgcaatac ggcgcgccgc tacgtacggt
    12961 cgaagtggtg gcgatggcag aagaagaagg cagccgcttc ccgtatgtct tctggggcag
    13021 taaaaatatc tttgggctgg cgaatcctga cgacgtgcgg aatatctgtg atgccaaagg
    13081 aaatagtttt gtcgatgcga tgaaggcttg cggatttact cttccgaacg ccccactaac
    13141 tccgcgtcag gatattaaag cctttgttga actgcatatt gaacagggct gtgtgctgga
    13201 aagtaatggg caatcaattg gcgtggtgaa tgcaattgtc gggcagcgtc gttatacggt
    13261 aacgctgaac ggcgaatcaa accatgcagg caccacgccg atgggttatc gtcgtgatac
    13321 agtttacgct ttcagtcgca tttgccatca gtcggtcgaa aaagcgaaaa ggatgggcga
    13381 tccgctggtt ctgacctttg gcaaagtaga gccgcgcccg aatacggtaa atgtggtgcc
    13441 gggtaaaacc acgttcacca ttgattgtcg tcataccgac gctgccgtgc tgcgcgattt
    13501 cacccaacag ttagaaaacg acatgcgggc gatttgcgat gaaatggaca ttggtattga
    13561 tatcgattta tggatggacg aagaacccgt gccgatgaat aaggagctgg tcgccaccct
    13621 gacagaattg tgtgaaagag aaaaactgaa ttaccgggtg atgcacagtg gtgccgggca
    13681 cgacgcgcaa attttcgcgc ctcgcgtacc aacctgcatg attttcattc ccagcatcaa
    13741 cgggatcagc cataacccgg cggaacgcac caatattacc gaccttgccg aaggggtcaa
    13801 aacgttggca ctcatgcttt atcaacttgc ctggcagaaa taaggagtca taaatgggat
    13861 atttaaataa cgtcaccggt taccgcgatg atttactggc taaccgtgcg attgttaaac
    13921 acggtaattt cgcactgtta accccggatg gtctggtaaa aaatattatt ccgggctttg
    13981 aaaattgtga cgcgacaatc ctctccacgc caaagctggg tgcctctttt gttgattatc
    14041 tggtcacact gcatcaaaac ggtggcaacc aacagggctt cggtggcgaa ggcattgaaa
    14101 cgttcctgta tgtgatctct ggaaatatca ctgccaaagc cgaaggcaaa acatttgcct
    14161 taagcgaagg tggctatctt tattgcccac caggctcctt aatgacgttt gttaacgccc
    14221 aggccgaaga cagccaaatc tttttatata agcgccgcta tgttccggta gaaggccatg
    14281 caccgtggct ggtttctggt aatgccagcg aactggaacg cattcactat gaaggtatgg
    14341 atgatgtgat cctgctggat ttcctgccca aagagttagg ttttgatatg aacatgcata
    14401 tcctctcttt tgcgccaggt gccagccacg gttatatcga aacacacgtt caggaacatg
    14461 gtgcctatat tctttccggt cagggggttt ataacctcga caataactgg atcccggtga
    14521 aaaaaggcga ttacatcttt atgggcgctt attctttaca ggctggttat ggcgtagggc
    14581 gtggtgaagc gttcagctat atttactcga aagattgcaa ccgcgacgta gagatttaag
    14641 tcataccact cgcctgatta tttttagcta tgttggattt tgccacggtt tataccgtgg
    14701 ctttttttgt agtaaattca ggtaaaaaaa gatgcgaaat aaatcgcatc cgctttaaag
    14761 gtaagggtca gtttttaatt ccctgaccta ttttaatggc gcaggcaata tttcgcgcgc
    14821 tgttgaagag attggtttca ccgctggcga gcacttcggc taaaggtgcc agacgaggca
    14881 aaatgctgaa taccgcgtca atgccgtact ggtgcaccac ttccacgcca tcacccaata
    14941 cgccagcaat cccaatcacc gggacattaa actgcttcgc caccgacgca acgcccagcg
    15001 gcgctttacc gcctgccgtt tgcgagtcaa tgcgcccttc cccggtgatc accagtgctg
    15061 cgccctgcac cgcctgctcc aggttgaccg cccgcagtac aatctcaata cccggtttaa
    15121 tatccgcatt gaggaatacc gccgccgcaa tacccatacc gcccgcagcg ccgccgcccg
    15181 ccatctggca gacattaatt tcagtttgct gttgtaaaac acgggcgtaa ttttgcagcc
    15241 cctgttcaag ttcttcgacc atctccggcg ttgccccttt ttgcgggcca aacaccgccg
    15301 ccgcgccgcg tgcccctacc agcgggttat caacgtcaca tgctacttca atatggcaat
    15361 tcgccagacg cggatcgcat tcatccatct caatgcttgc cacgcgcgct aaattaccgc
    15421 cgttagccgc cagcacctgg ccgtcggcat caaggaaacg cacaccaagc gcctgagcca
    15481 tgcccatacc gccgtcgacc gtcgcactgc cgccaatgcc gagaataata tggcgaatgc
    15541 cgttatccag cgcatgacga attaactccc ccgtaccaaa actggaggcc agcaacggat
    15601 tacgcttttc aggcgcgacc agcatcaggc cacttgctgc cgccatctca atcaccgccg
    15661 ttttcccgtc gccggtaagg ccataaaaag cattcactgt ttcgcccatc ggcccgcaga
    15721 cttcaagcgt cacgatgttg ccgcccgtcg cggcgaccat cgcatccacc gtcccttcgc
    15781 cgccatccgc tatcggcaaa cagatatagt tcgcatcggg aaagagggtc gaaaacccgg
    15841 ctttaattgc ctgacaacat ttttctgcac ttaagctctc tttaaaagag tctggcgcaa
    15901 tgacaatctt cattatcaat cctttttcaa caaattattc gatgcgttcc cacggaataa
    15961 gattatccat aagaacagcc agtaaaattc cgaccagtaa gccgttactg agcagaggac
    16021 gcagcgttaa tggaaggtct tgcagataca caggcggtaa tgccattaaa aatatgccga
    16081 caaataacgg caatgcgagt cgataaatat tgcgagcggt aaaggttatt tgctggctaa
    16141 acaccagcgc ggaaaagagt aaaggcagat aagaaaccag catgaccgcg ctactcacgg
    16201 gtaaagggat actgcaaaac agtcgtgtga gtgccggaac cagcgccacc agcaggcaaa
    16261 taacgctgcc ataaataaac gaacgccgcg tgtaatcgcc tgtctgggtt aataaaccaa
    16321 tggatgaaac aaacggtgaa aatggaatta ccgccagcgg tacggttatc agcgtcataa
    16381 atccggtcgc cacaaagctg cgacgataac gcgtattccc gcgccctgct gcggataaaa
    16441 aacatccgtg ccccgaatcg caccgtaggt attgctgata ttcaccagac ctgtaatcac
    16501 cgccgtcaga ataattcccg gagacaaagc acctccactg ccgagcggga accactgcca
    16561 gtgcaactca ccggagagcg agtgcgaaga aggaaagcaa aagtaccaca acaaccagcc
    16621 ggttatggtg ccgaccagca ggccataacg ggcaaaacgt tgcggcagga agataatcat
    16681 ccccagtacc aggcacatca ccgccaccga gagcgcgaac ggcggtaact gaattttaaa
    16741 atttgggtcg gctatgccaa acggcagccc gagcatacct ttgaaaaaga tagtggtcag
    16801 ctgcgcgccc agcatcaaca taaacaagac catcaccgac ggcgtaaata accgtgccag
    16861 gcgatggcct aatccgctaa aaccaatcaa catcgtcagc acgccggaga gcgcaatccc
    16921 caccgccagg ctggtagcga tatcgttgat cggtgtcccg cgcgatgctt cgccaagggt
    16981 gatagtaagg atggttcccc accacaggcc acccggccct tccataatag cgcgacgatg
    17041 accgcagaac gcctgagcga agcaggccag tgcggtagca agaaaagcat attgcgtgag
    17101 cgtaagcagg ctactttgcg gcaactgaaa agcagaaagt agcgtaggag gaaccacaac
    17161 cgtgttgcaa aaaataaaga aaaaccactg aaatcctgat aacaggcttt cgcggctcac
    17221 tgcaaaattg aacataaaca caccttaacc ggagaatgcc cgccgcaagg acgggcattg
    17281 caggggccag attactgctg atgtttaagg ataaattgac ctttcggcgc aacagggaag
    17341 ccctgttcaa tgtcgtaaat cacatcacca cgtaagatgg ttttcgtgat acgcgcgcca
    17401 atggtacggc caacatacgg gctgactttg tggcgatatt ccagatcgtc attggtaaga
    17461 acatagctgc tattcggctg aatgaagacg aagtcggcat cttttcctgg ggcgatacgg
    17521 cctttttgct gcagaccgaa aatatctgct gcgttagtcg ccattaattt gccgaacatt
    17581 ggcagagaca ttccgcgttt ctgtaccgct tcatcgaaca tcacgtccat acagttttgc
    17641 agaccggcaa taccgcccca tgcttccatg atgttgccgg ctttcatttc cggagggcat
    17701 ggtgagtggt cggaaaccag gcagtcgatt tcaccgttaa acagtttttc ccacatgcct
    17761 ttctggtttt ccagatcgcg gatcggcggt gaacacttcg ccagagtgcc gatttcttcg
    17821 aactgatcgg tatccagtac aaagtaatgc gggcaggatt cacaagtaac gtcctgacct
    17881 tcctgacgtg cacgagtcac ttcttcaacg ccttccgggc tgctgatatg gcaaacgtgc
    17941 agacggcaac cggcaacttt cgccagatac agcacgcggc gaatcgcttc cacttcggta
    18001 aataccggac gcgaagccac atagtcatgg gcagttacgc gaccttcacg tttcgcttct
    18061 tcgcccagtg cgtcacagat cagcgcgttt tcgcagtgca ccagcaccgg ctgccccagt
    18121 tcgcccagct tctgcgcacc tttgaagaac tgccagtcgt ttacatcacg gaagtcgttg
    18181 tcgataccgc gatcgccaca ggtcgcaacg aagcatttga agccgacaac gcccacttca
    18241 tccagctcat gcagacggtc gatgttgtaa gacaccaggc caccgagttg tgccgcatcg
    18301 atagtcagct tgcctttagc ggcatcgaac ttcagttcaa ttgaagcgcg gtcaaccgtt
    18361 gcaggcagct ggttgagcgg catttcgatc atggtggtga taccaccttt tgccgctgcg
    18421 cgagtaccgg tttcataacc ttcccagtgg ctacgacccg gttcagaaat atgggtgtgc
    18481 gcatcaacca tgcccggcga aaccaccaga ccagacgcat ccataacttc ttttgcatcg
    18541 cccagatcct gaccgatagc agcaattttc ccgcctttaa cggcgatatc tacaacgcga
    18601 gcttcgtttt ctaaaataac ggtgccgttt ttaatgatta aatcaaaaga cataacaaac
    18661 tccttgtaaa atacagattg gtttttaact caatgttcaa tatcgggatt aattaaccta
    18721 tggttttttg ctctcctgtt ttttctgctg ttgtacgttt ctttaataag gcgtaggccg
    18781 caaaggcgac gatgacgccg acaaaccatg aaacacgcga taacggttcc ataaagtgaa
    18841 taaacttacc gccaagagat aaaataacgg ccaccagagt tactgaaaac gcagtgaggt
    18901 taaaaccgtt atcgtaatat ttataatcgc caggtgcggt atacagttca tcaagattaa
    18961 tttgtccgcg catcaccaca aaataatgcg ccatcatgac accaattacc ggaccaagca
    19021 ttccgccgat aatatcgagg aaaagataaa tgctgtcctg attttccatt aatttccacg
    19081 ggcagatcag caagctgata atactggcaa tcagtacgcc gtttttatag gtcagttttg
    19141 tcggtgcaat ggcggcaatc tgataaccgg ctggaataat attaccggtc gcgttagtgg
    19201 agatagttgt catcagaatg accagtaccg caaagaacga ggcgaacagg ctgtcccaac
    19261 gctgaacaat atccagcacg ttccaggtat cagcgccgta gtgaatactg gctccggcaa
    19321 taatacatac cccggcgacc gcaaacagaa tataggccac aactaaaccc agcgtttgcc
    19381 ccagcgcctg ctcacgaaac gagtgggcgt tttgcgtaaa gtcggatgcg ctcaccgccg
    19441 gtgccgccca gaccgcaact accgcgttaa tcaccaccag gaacaggaag ccaccgtttt
    19501 ctgctttctg aataccgctc ggaatgtagt caaagattgg accgatcccg accagtgaaa
    19561 tcgcccaaat cgccataccg ccgaaaacga tatagatgca cgggttaaga atggcagtga
    19621 atttatttaa aactttgcca ccgccaaaac ctataccaac gttgaccagc cagaagatta
    19681 agaaagtaat taagcccggt agagaaaggc ctaacagagt gaaatcacca ccgagagtta
    19741 aaaatcccgg ccagattttg ccaatcagaa tcaagcaggc cagtgacccc gcgtaacatt
    19801 gcaaaccaaa ccacatgatg gcggcaattc cgccccttaa taatccggga aacagtgcac
    19861 cacgtacacc gtaagaagca cgcaggatca tggcaaaagg cacaccgtat ttactgcccg
    19921 cagcaccgtt taataccatt accgcggcaa tgaaaaaggc gctgaggata attgccagca
    19981 taatactaaa ggtagacaag ccgagaataa aaaagccgcc gaccatcaca taattgggaa
    20041 cgttatgaac cgaacccatc cataaggtaa aatagttaaa tgttttccag gtccgctggc
    20101 tttgcgtttt cggcaataga tcttcgctat agccgcgttg ctggaatagt tttctctgat
    20161 gttccataaa tccctcttaa tttcacgtat cgaataacgt tgttttaatc tagctatgag
    20221 gaacaaat
//
LOCUS       DAZGLJ010000070        19856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000070 DAZGLJ010000000
VERSION     DAZGLJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.070"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1036)
                     /gene="wzzE"
                     /locus_tag="ACHK49_002229"
     CDS             complement(<1..1036)
                     /gene="wzzE"
                     /locus_tag="ACHK49_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO9262873.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNEELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRR"
     gene            complement(1048..2151)
                     /gene="wecA"
                     /locus_tag="ACHK49_002230"
     CDS             complement(1048..2151)
                     /gene="wecA"
                     /locus_tag="ACHK49_002230"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO9262874.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(2391..3650)
                     /gene="rho"
                     /locus_tag="ACHK49_002231"
     CDS             complement(2391..3650)
                     /gene="rho"
                     /locus_tag="ACHK49_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO9262875.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(3735..3836)
                     /gene="rhoL"
                     /locus_tag="ACHK49_002232"
     CDS             complement(3735..3836)
                     /gene="rhoL"
                     /locus_tag="ACHK49_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO9262876.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(3977..4306)
                     /gene="trxA"
                     /locus_tag="ACHK49_002233"
     CDS             complement(3977..4306)
                     /gene="trxA"
                     /locus_tag="ACHK49_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO9262877.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            4437..5702
                     /gene="rhlB"
                     /locus_tag="ACHK49_002234"
     CDS             4437..5702
                     /gene="rhlB"
                     /locus_tag="ACHK49_002234"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO9262878.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKYRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            5836..7320
                     /gene="gppA"
                     /locus_tag="ACHK49_002235"
     CDS             5836..7320
                     /gene="gppA"
                     /locus_tag="ACHK49_002235"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO9262879.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(7367..9388)
                     /gene="rep"
                     /locus_tag="ACHK49_002236"
     CDS             complement(7367..9388)
                     /gene="rep"
                     /locus_tag="ACHK49_002236"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO9262880.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            9605..10054
                     /locus_tag="ACHK49_002237"
     CDS             9605..10054
                     /locus_tag="ACHK49_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO9262881.1"
                     /translation="MAINFSPKVGEIQECNFGNYPVIQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            10253..10534
                     /gene="ppiC"
                     /locus_tag="ACHK49_002238"
     CDS             10253..10534
                     /gene="ppiC"
                     /locus_tag="ACHK49_002238"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO9262882.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(10621..12096)
                     /gene="ilvC"
                     /locus_tag="ACHK49_002239"
     CDS             complement(10621..12096)
                     /gene="ilvC"
                     /locus_tag="ACHK49_002239"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO9262883.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            12246..13139
                     /gene="ilvY"
                     /locus_tag="ACHK49_002240"
     CDS             12246..13139
                     /gene="ilvY"
                     /locus_tag="ACHK49_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO9262884.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSAEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(13191..14735)
                     /gene="ilvA"
                     /locus_tag="ACHK49_002241"
     CDS             complement(13191..14735)
                     /gene="ilvA"
                     /locus_tag="ACHK49_002241"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO9262885.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(14738..16588)
                     /gene="ilvD"
                     /locus_tag="ACHK49_002242"
     CDS             complement(14738..16588)
                     /gene="ilvD"
                     /locus_tag="ACHK49_002242"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO9262886.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQDARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(16653..17582)
                     /gene="ilvE"
                     /locus_tag="ACHK49_002243"
     CDS             complement(16653..17582)
                     /gene="ilvE"
                     /locus_tag="ACHK49_002243"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO9262887.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(17602..17865)
                     /gene="ilvM"
                     /locus_tag="ACHK49_002244"
     CDS             complement(17602..17865)
                     /gene="ilvM"
                     /locus_tag="ACHK49_002244"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO9262888.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(17862..19508)
                     /gene="ilvG"
                     /locus_tag="ACHK49_002245"
     CDS             complement(17862..19508)
                     /gene="ilvG"
                     /locus_tag="ACHK49_002245"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO9262889.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(19511..19561)
                     /gene="ilvX"
                     /locus_tag="ACHK49_002246"
     CDS             complement(19511..19561)
                     /gene="ilvX"
                     /locus_tag="ACHK49_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO9262890.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(19648..19746)
                     /gene="ilvL"
                     /locus_tag="ACHK49_002247"
     CDS             complement(19648..19746)
                     /gene="ilvL"
                     /locus_tag="ACHK49_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO9262891.1"
                     /translation="MTALLRVISLVMISVVVIIIPPCGAALGRGKA"
BASE COUNT         4655 a         5346 c         5115 g         4740 t
ORIGIN      
        1 aacggcgggt taatgcgaca ccagccccga tcagcccccc gacaatgccc cacataatca
       61 tcaggaaggc acgacgtggg ctatcgcgtt ttaccggttc ttccggcgta cgcaaatagc
      121 gataggtctg aaaacgcgga tccagagttg gaccaacatt cagggtgttt aacatggccc
      181 gattctgatc atagtcgaga tcaaaggccg gaccgacggc ctgtaaattt tccagtcgag
      241 cctggagcat tggacgccca agcaggaaca tttctgaatc aggtaattcc tcggcaggca
      301 catctgtcgc actgcgcgaa atattatgct gctcagcaat tttcagcgcc tgctcaatgc
      361 tgttcatccg gcggtcgtag atggctttcg ccacctcttc ctgacgcttc acctgagctt
      421 tcatctggat ggtgcgtgcc gcccatgcgc ctttcagctc ctcattcaga tggctggctg
      481 cacgctggct ggcaaaagca acatactgac gtaacaggtt attagcgtca ggcgcggttt
      541 cggcaataag cttcacgctg tcattgaccg cgcgggtaaa gtctccgggg ataaactgga
      601 tgttgttaat catttcatcc agcaacgccg catcggcttt gctgttgccc accatccgct
      661 gcttgtaata gtcggtttgc agccagaact ctctgcgggt atcccacgag gccagttgca
      721 taacaaactc tttataggct tcgtccatga ccgatggttg gtcggcagaa gccatgtttg
      781 aacggacatc caggttacgc aaaaattgct gctgcgagta atatcccccc agcatattca
      841 ccgttggacg atcggtaatc gccgtcgcgc tccactcttg tcgagcaaaa aaagtatacg
      901 ccagcgcgat taacgcaaac gccagcccca tgccaataat ccatagcttc ccagcccaca
      961 aggtacgaaa caacccacga atatccagtt cattttcagc gtcttcggcc ggtttcccag
     1021 gcattggttg tgtcatcaca tcctcattta tttggttaaa ttggggctgc caccacgatt
     1081 tctacgcagt ctgcgtttta cgcgcttaat aaagcgagca actttccagg cacgcttaat
     1141 gcaatatcca tagaggaaga atgctagcaa aaagagcacc agcatgaccc actccgggac
     1201 aaaatgagaa tattctgcca gcacgccaat ggaagcgagc agtgctgcgg caagggtaat
     1261 cagcacaaac gcctgacggg aagtaaaccc ggcacgcatg atcaaatggt gaatatgctg
     1321 acggtcagga gagaatgggc tcatgccttt acgcaggcga cggtacataa tcgccaccat
     1381 atccattagc ggaatggcga ttatccacaa agcggtaacc gggctgatgg gatgggtttt
     1441 gccctgggtc gtttcgagca ggatccagat aacggtaaaa ccaatcagcg tactgcccgc
     1501 atcacccata aagactttgt agcggcgacc caggatacca aggttaagca tgatgtatgg
     1561 caggatggcg gcgatcatcg caaagcacca gattgcgagg ctggtttgcc cgtcgaacca
     1621 caaaatcata ccgattgctg caaacgagac gcaggacaac ccgcccagca agccatcaat
     1681 gccatcaacc atgttgaacg cattaatggc cgcccagacg gcaaatagcg tcaggaagta
     1741 accaaacggt ccgagcacca tctcccagga gccaaagata taacccaggc tactgagata
     1801 aagtttgccg aacaccatca taacaatgcc aacagcggcc tgtatggtgg cacggatttt
     1861 tacgctgata tcaaaacggt catccagcgc gccaatgaaa acaagcacac cggcacaagc
     1921 gagatagaga gatgcatgcg gaatatagta atcgacaatt ccgaacgtga agcaaatccc
     1981 tgcgtaaacc gaaatccccc caacgagagg tatcaatccc tggtgacgtt tgcggaagtt
     2041 tggtttatcc actaaaccga ctttttttgc cactttacgg gcaaaaaaca gaaacagtgt
     2101 cgtgaataaa aaaatactga tgagatcagt actcactgtc agtaaattca caatgcatgc
     2161 tctcagagaa aattattagc agaagtataa ccacgaagac ctttattcag aagggaaagt
     2221 ccgttccgaa cccaccagtc ctgtgcaaaa attaaccatt tgatatgcaa attgcataac
     2281 atgccagcaa attccaggaa ttttcctaac atcgccagcg cggcattaag attacagata
     2341 taaaagcaaa acgccacgta aacacgtggc gtttttggca taagacaaat ttatgagcgt
     2401 ttcatcattt cgaagaaatc gtcattggtc ttagtcatgg ccagtttatt aatgaggaat
     2461 tccattgcat cgatttcgcc catcgggtga atgattttgc gcaggatcca cattttctgc
     2521 agttcttcct gagtcgtgag cagctcttct ttacgggtac cggaacggtt gtagtcgata
     2581 gccgggaaga cgcgtttttc agcgatctta cgagagaggt gcagttccat gttgcctgta
     2641 cctttaaact cttcgtaaat aacttcgtcc attttagaac cggtatcgat aagcgccgtc
     2701 gcgataatgg tcaggctgcc gccctcttcc acgttacgcg ccgcaccgaa gaagcgtttc
     2761 ggacgatgaa gggcattggc gtccacacca ccggtcaaca ctttacctga cgccggaaca
     2821 acagtgttgt aagcgcgcgc cagacgagtg atggagtcga gcagaatgat aacgtctttc
     2881 ttgtgctcaa ccaggcgttt ggccttctcg atcaccattt ccgcaacctg aacgtggcga
     2941 gatgcgggtt cgtcaaaggt agaagcaaca acttcacctt ttaccagacg ctgcatctcg
     3001 gttacttctt ccggacgttc gtcgatcagc agaaccatca gcacacaatc cgggtggttg
     3061 taagcaatgc tctgagcaat gttctgcagc agcatagttt taccggcttt cggcggtgcc
     3121 acaatcagac cacgctgacc acgaccgata ggtgatgcca gatccagtac gcgagcggtt
     3181 aaatcttcag tagaaccgtt accacgttcc atacgcagac gagagtttgc gtgcagcggg
     3241 gttaagttct caaagaggat tttgttgcgg gcgttttcag gtttgtcgaa gttaacttcg
     3301 ttaactttca gcagcgcaaa atagcgttca ccttctttcg gcgggcgaat cttaccagag
     3361 atggtatcac cagtgcggag gttgaaacgg cggatttggc taggggaaac gtagatgtca
     3421 tcaggaccgg cgaggtagga gctgtctgcg gaacggagga aaccaaatcc atcctgcaat
     3481 atctccagta cgccatcacc aaagatatct tcgccactct ttgcgtgctg cttcaggatg
     3541 gcaaaaataa tgtcctgctt acgcatacga gccaggtttt ccagccccat attttcgccg
     3601 agagtgatca gctcagaaac cggcgtattc tttaattcgg taagattcat agtggtgtga
     3661 gttcttaaac ttggggtaaa tctcgaactt aatgttgtga atggtatggc aggatcatcc
     3721 atgcctgttt acggtcatcg actcatgtct tttcgctgcc tggtcacagg agagtacgca
     3781 gaactgaaac gacaagacgg attgagtgac gagccagaaa tctgttcact tcgcatttaa
     3841 gataaaacgg gaagcgttgg aaattacaag attcaaactt aataaggtat gtttaatacg
     3901 aagtcaacac taagttagca tgactcacgc cgggcgtcca gtttttagcg acggggcacc
     3961 cgaacatgaa attcccttac gccaggttag cgtcgaggaa ctctttcaac tgacctttag
     4021 acagtgcacc cactttggtt gccgccactt caccgttttt gaacagcagc agagtcggga
     4081 taccacggat gccatatttc ggcgcagtgc cagggttttg atcgatgttc agttttgcaa
     4141 cggtcagttt gccctgatat tcgtcagcga tttcatccag aatcggggcg atcattttgc
     4201 acggaccgca ccactctgcc cagaaatcga cgaggatcgc cccgtccgct ttgagtacat
     4261 ccgtgtcaaa actgtcgtca gtcaggtgaa taattttatc gctcatatat aactccacag
     4321 gaataagcct ggcgtgttgg tttcgttgtt ggtgtaacat taaccaacta aaggttgact
     4381 ttatttcacc ggatacgctt tcgtaaagca atagtaagct gatattctac cacactatga
     4441 gcaaaacaca tttaacagaa cagaagtttt ccgacttcgc cctgcatccg aaggttgtag
     4501 aagcccttga aaaaaaaggg tttcataact gtacgcccat tcaggcactg gcccttccgc
     4561 tgacgctggc gggtcgtgac gtagccgggc aggcgcaaac cggtaccggg aaaacgatgg
     4621 cgtttcttac gtcaacgttt cattatcttc tctctcatcc tgcgattgcc gatcgcaagg
     4681 tgaatcaacc acgtgcctta attatggcac cgacgcgtga acttgccgtg cagatccatg
     4741 ccgacgcaga accgctggcg gaagctaccg gcctgaagct gggtctggct tacggtggtg
     4801 atggctacga caaacagctg aaagtgctgg aaagcggcgt tgacattctg attggcacta
     4861 cggggcgttt aattgactac gccaagcaga accacattaa cctcggtgcc attcaggtgg
     4921 tggtactgga cgaagccgat cgcatgtacg atctgggctt tattaaagat atccgctggc
     4981 tgttccgccg tatgccgcct gcaaaccagc gcctcaacat gctgttctcc gccacgcttt
     5041 cgtaccgggt acgtgaactg gcgttcgagc agatgaacaa tgccgaatat attgaagtgg
     5101 aaccggaaca gaaaacgggc caccgtatta aagaagagct tttctaccct tctaacgaag
     5161 aaaaaatgcg tttgctgcaa acgctgatcg aagaagagtg gccagacaga gcgattattt
     5221 tcgccaatac caaataccgt tgtgaagaga tctggggcca cctggcggca gatggtcatc
     5281 gtgtcggttt gttgacaggc gatgtcgcgc agaaaaaacg tctgcgtatt cttgatgaat
     5341 ttacccgtgg cgatctggat attctggttg ccaccgacgt tgccgcgcgt ggtttgcata
     5401 ttccggcagt gacgcacgtc tttaactacg atttacccga tgactgtgaa gattacgttc
     5461 accgtattgg tcgtacaggt cgcgcaggcg caagcggtca ctctatcagc ctggcgtgtg
     5521 aagagtatgc attgaatttg cctgctattg agacctatat tggtcactca attccggtaa
     5581 gcaaatacaa tccggacgca ttgatgaccg atctgccaaa accgctgcgc ctcacgcgcc
     5641 cacgtacagg caatggtccg cgtcgtactg gcgctccgcg taatcgtcgt cgttcaggtt
     5701 aaataaaatg tcatactcaa aatcattcaa actgtatcaa gatggcaaga gaatgaatcc
     5761 cgatgagcat actgtagtaa gtgattcagg tgaaagaatg cagccaacac agagacagat
     5821 tgaaggatga agagtatggg ttccacctcg tcgctgtatg cagccattga tctcggttcg
     5881 aatagttttc atatgctggt tgtgcgcgag gtggctggaa gcatccagac gctgacgcga
     5941 ataaaacgca aagtgcgtct ggctgctggc ctgaatagcg aaaatgccct gtctaatgaa
     6001 gcaatggagc gcggttggca atgtctgcgc ctgtttgctg aacgtctgca agatatccct
     6061 ccctcgcaaa ttcgcgttgt cgctacggcg acattacgtc ttgccgtcaa tgcgggtgat
     6121 tttattgcca aagcgcagga aatcctcggt tgtccggtac aggtgatcag cggtgaagag
     6181 gaagcacgtc tcatttatca gggcgttgct cacacaactg gcggtgccga tcagcgcctg
     6241 gtggtggata taggcggtgc cagtactgaa ctggtaaccg gcacgggtgc acaaaccacc
     6301 tcgttgttca gcctgtcgat gggctgcgtc acctggctgg aacgctattt tgccgatcgt
     6361 aatctggggc aggaaaattt tgatgctgca gaaaaagcgg cacgcgaagt gttacgtccg
     6421 gttgccgatg aattacggta tcacggctgg aaagtgtgcg ttggtgcttc cggcaccgtg
     6481 caggcgttac aggaaatcat gatggcacag gggatggatg aacgcattac cctggaaaag
     6541 ttgcagcaat tgaaacagcg agccattcat tgcggtcggc tggaggaact ggagattgac
     6601 gggctgacgc tggaacgtgc gttagtgttc ccgagtggtc tggcgatcct gatcgccatt
     6661 tttaccgaac tgaatattca gtgtatgacc ctggcgggcg gtgcgctgcg tgaaggcctg
     6721 gtctacggca tgttgcatct ggccgtcgag caggatattc gcagccgtac gctgcgtaat
     6781 attcagcgcc gctttatgat cgatattgat caggcgcagc gcgtagccaa agttgcggct
     6841 aacttcttcg atcaggtgga aaaggaatgg catcttgaag caataagccg cgatttgctc
     6901 atcagcgcct gtcagcttca tgaaatcggc ctgagcgttg actttaaaca agcgccgcaa
     6961 cacgctgctt atctggtgcg taatctggat cttcccggtt ttacccccgc acagaaaaaa
     7021 ttgctggcga cgctactgct caaccagact aatccggtcg atctctcatc gctgcatcag
     7081 caaaatgccg tacctccgcg cgtcgcagaa caactctgcc gtttactccg cctggccatc
     7141 atttttgcca gccgtcgccg tgacgatctc gtgccagaga tgacattaca ggctaaccat
     7201 gaactgttga ccttgacgct tccgcaaggt tggctaaccc aacatccgct gggtaaagag
     7261 attattgctc aggaaagcca gtggcagagc tatgtccact ggccgctgga agtgcattaa
     7321 tgagtaagtg ccggatgcga tgctgacgca tcttttccgg ccttgattat ttccctcgtt
     7381 ttgccgccat catcgctttc agattcgcca gatggctttg ccctttctgc atccgttctt
     7441 cggcgctgac cactttgcgc tcctgttccc aaatcagatc atcctgcggc agctccagca
     7501 aaaagcggct cggctccggg cgcaccagct cgccgtactg acggcgttct ttacacagcg
     7561 taaaggtcaa ttccttctgg gcgcgggtaa tgccgacata ggccagccgc cgctcctcat
     7621 cgatattatc ttcatcgatg ctgctctggt gcggcaaaaa cccttcttcc ataccgacca
     7681 tgtagacata aggaaactcc agcccttttg acgcgtgcag agtcatcagt tgcacctgat
     7741 ccagctcttc ttcactctca ccacgctcca tcatgtcgcg caaagtaaag cgcgtcacca
     7801 cctgggtgag cgtcatcggc tcatccagtt cgctgccttc cagcatctcc gtcatccagc
     7861 taaacagttg gttgacgttc ttcatgcgca tttcggcggc tttcgggctg ggcgatgttt
     7921 cgtacagcca ggattcataa tccatgccgt ggatcagatc gcgcaccgcg gcaatcggct
     7981 cccgctccgc cagacgctgg atttctgcca accagtgagt aaagcgggtc aatgcttcat
     8041 aaccacgtcc gctaagcgtc tgactcaggc ccatatcaaa gctggcggta aacatgcttt
     8101 tattgcgcgt catcgcccac tcacccagct ttttcagcgt cgccgggcca atctctctct
     8161 tcggcgtgtt aacgatacgc agaaatgcgc tgtcatcgtc cgggttagtc agcacgcgca
     8221 gataagccag caagtccttg atttcaggac gagagaaaaa cgacgtacca ccagaaattt
     8281 tgtacgggat gcggttttgc atcaggaatt tttcaaacac tcgcgactga tggttaccgc
     8341 gataaagaat agcgtaatct ttgtactgcg ttttattgac gaagtgatgc gcaatcagtt
     8401 caccagtgac gcgctcggct tcatgttctt cgttattcgc gcttaaaact ttaagctctg
     8461 tgccgtaacc cagttcggag aacagacgct tctcaaagac gtgcgggtta ttggcgatca
     8521 ggatgttcgc cgctttcaaa atacgtccgg aagagcggta gttctgctca agcttaatca
     8581 ccttcagcgc cggaaaatcc tgactcagca gcaccaggtt ttgtggacgt gcgccgcgcc
     8641 aggagtagat cgactggtcg tcatcgccca ccacggtaaa gcgcgcgcgg ctgcccacca
     8701 gcagtttcac cagttcatac tggctggtgt tggtgtcctg atactcatcc accagcagat
     8761 agcgaatttt gttctgccag cgctcgcgga cttcttcatt gcgttgcagc agcaacgtcg
     8821 gcaataaaat cagatcatcg aagtcgagaa cattgcacgc tttcaggtgt gcatcataca
     8881 gcccataaca atgggcaaaa atacgatccc gctcgccctt tgcctctgcc gccgcctgcg
     8941 ccggagtttt gagatcgttc ttccagttgg agatggtcga aatcagctgt tgcaggagaa
     9001 ctttgtcatc ttcaatcagc ccctcggtca actctttaag caaagcaagc tgatcggtat
     9061 cgtcaaacag cgaaaagttc gccttcatcc caagcgccgc atactcgcgt ttgatgatat
     9121 ccaaccccag cgtgtgaaaa gtggagatca tcaacccgcg cgcctctttg cgacccagcg
     9181 tctgcccgac acgctctttc atctcgcgcg ccgccttatt ggtaaaggtc accgccgcga
     9241 tatgtcgcgc ctgataaccg caaccgcgga tcagatgggc gattttattg gtgatcacac
     9301 gagttttacc ggaacccgcg cccgccagca ccaggcaggg gccggtaacg aattcgacag
     9361 cttgttgttg gccggggttt agacgcatag gtgtattgct caatcttcga acggggggag
     9421 gattgtagca gaatcgcatt tgaatgtgac cgcgtgtaag ccgctaaccg gttggctgcg
     9481 gatgtttaaa tcagagagag aagaccgaaa tcggagttca tcgtgaatcc attagactta
     9541 gaaaatatcg ggtcgtaacg ttgtgttatt actttttcag agtttactaa ggagaggttt
     9601 actcgtggcg attaatttca gccctaaggt tggtgaaata caggaatgca actttggtaa
     9661 ctatcctgtt atccagaatg gacagttcag caccacgtat tacgatggcc gcattcctcc
     9721 tgaaatgata aaaaacaggt tagttgtcgt gctcaacgga aaaattaatg gcaacgcatg
     9781 tatcgtcgtt ccattatcaa ccactcgcga tcatgacaag ttaaatcgtg gaatgcatgt
     9841 tgaaatagcc agtaatgtta tcaatggcct gcaattcttt gatcagcaga ttcgttgggc
     9901 caaagccgac ctggtacaac aagtcagtcg aaaccgatta aatagagcaa ggacttacag
     9961 agggtatctt aatcaatgtt tgccacatga gttggttgca gacatacaga gggcggtcat
    10021 caagtcaatc aatgccatat cgctcataaa ttgacaataa aataaccatc cccgataatg
    10081 actttgtagc ccatagaggc cctaagtttt ctccccttga gggacctgcg aagcccggat
    10141 tttccgggct tttgccatat atgtaccagc ccctccctga ctgacaatta cgttagcatc
    10201 tgcggatcat gctaaaatcg ccgcctgaca attattcact caaggcacga tcatggcaaa
    10261 aacagcagca gcactgcata tccttgtaaa agaagagaaa ctggctctgg atcttctcga
    10321 gcagattaag aacggtgccg atttcggcaa gctggcgaag aaacactcta tttgcccgtc
    10381 aggcaaacgc ggcggtgatt taggtgaatt ccgccagggt cagatggttc cggcgttcga
    10441 taaagtggtt ttctcttgtc cggtactgga gccgaccggc ccgctgcaca cccagttcgg
    10501 atatcacatc attaaggtgc tgtaccgcaa ctaatagcaa ggccttctcc aggagaaggc
    10561 cttgagtgtt ttctccctct ccctgtggga gagggtcggg gtgagggcat cagcgcgcac
    10621 ttaacccgca acagcaatac gtttcatatc tgtcatatag ccgcgcagtt tcttacctac
    10681 ctgctcaatc gcatggctgc gaatcgcttc gttcacatca cgcagttgcg cgttatctac
    10741 cgcgccttcc ggaatagctt tacccaagtc gcccggttgc agctctgcca taaacggttt
    10801 cagcaacggc acacaagcgt aagagaacag atagttaccg tactcagcgg tatcagagat
    10861 aaccacgttc atttcgtaca gacgcttacg ggcgatggtg ttggcaatca gcggcagctc
    10921 gtgcagtgat tcataatatg cagactcttc aatgatgccg gaatcgacca tggtttcgaa
    10981 cgccagttca acgcccgctt ttaccatcgc gatcatcagt acgcctttat cgaagtactc
    11041 ctgctcgccg attttgcctt catactgcgg cgcggtttca aacgcggttt tgccggtctc
    11101 ttcacgccag gtcagcagtt tcttatcatc gttggcccag tccgccatca taccggaaga
    11161 gaattcgccg gagatgatgt cgtccatatg tttctggaac agcggtgcca tgatctcttt
    11221 cagctgttct gacagcgcat aagcacgcag tttcgccggg ttagagagac ggtccatcat
    11281 cagggtgatg ccgccctgct tcagcgcttc ggtgatggtt tcccaaccga actgaattag
    11341 tttttctgcg tatgccggat cggtgccctc ttccaccagc ttgtcgaagc acagcagcga
    11401 gccagcctgc aacataccgc acaggatggt ttgctcaccc atcaggtcag atttcacttc
    11461 cgcaacgaag gacgattcca gcacgcccgc acggtgacca ccggttgctg ccgcccatgc
    11521 tttagcgatc gccatacctt cgcctttcgg atcgttttcc gggtgaacgg caatcagcgt
    11581 cggtacgccg aatccacgtt tgtactcttc acgcacttcg gtgccagggc atttcggcgc
    11641 aaccatgact acggtgatat ctttacggat ctgctcgccc acttcgacga tgttgaaacc
    11701 gtgcgagtaa cccagcgccg cgccgtcttt catcagtggc tgtacggtgc gcactacatc
    11761 agagtgctgc ttgtccggcg tcaggttaac caccagatcc gcctgcggga tcagttcttc
    11821 gtaagtacca actttaaaac cgttttcggt cgctttacgc caggacgcgc gcttctcggc
    11881 aatcgcttct ttacgcagag cgtaggagat atccagacca gaatcacgca tgttcaggcc
    11941 ctggttcaga ccctgtgcgc cacagccgac gatgactact tttttaccct gaaggtagct
    12001 cgcgccatcg gcgaattcat cgcgccccat aaagcgacat ttgcccagct gtgccagctg
    12061 ctgacgcaga ttcagtgtat tgaagtagtt agccatggtg attcctcgtg atgttgtgct
    12121 tcttattgtt cggttcgcgt ttgcgagatt gaattcacta tatgacagga aatttattgc
    12181 ggaaattgat atattcacaa cgtcacattg caatttttgc aacgtcaaca tcgagggctg
    12241 cgccattgga tttacgcgat ctgaaaacct tcctgcatct ggcggaaagc cgccattttg
    12301 gccgcagcgc gcgggcgatg cacgttagcc catccacgct ctcacggcag attcagcgcc
    12361 tggaagaaga tcttggtcag ccgctatttg tgcgcgataa ccgcacggta acgctgacgg
    12421 aagcgggcga agagctgcgc gttttcgccc agcaaacgct gttgcagtat cagcaattgc
    12481 gccacaccat cgatcagcaa gggccgtcgc tctctggcga attacatatc ttctgctcgg
    12541 tgaccgctgc ctacagccat ctgccgccga ttctggatcg cttccgcgcg gaacacccgt
    12601 cggtggagat caaactcacc actggtgatg cggcagatgc aatggaaaaa gtggtcacgg
    12661 gagaagcgga tctggcgatt gccgggaaac cggagacgct acccggcgct gtggcgtttt
    12721 cgatgctgga gaatctggca gtagtgctga ttgcgcccgc gctgccctgc ccggtgcgta
    12781 accaggtttc ggcagagaag ccggactggt ctacagtgcc gtttatcatg gccgatcagg
    12841 gaccggtgcg tcgccgcatt gaactgtggt ttcgacgcaa taaaatcagc aacccgatga
    12901 tttacgccac ggttggcggg catgaagcga tggtatcgat ggtggcgctc ggctgtggtg
    12961 tggcgctgtt gccggaagta gtgctggaaa acagccccga accggtgcgt aaccgcgtga
    13021 tgattttaga gcgcagcgat gaaaaaacgc cgtttgaact gggcgtgtgt gcacaaaaaa
    13081 agcggctgca tgagccgctg attgaggcgt tctggaggat tttgccgaac cacaaatgac
    13141 gttgtcgcgc gggtaggcct gataagcgaa gcgctatcag gcatttttcc ctaacccgcc
    13201 aaaaagaacc tgaacgccgg gttattggtt tcgtcgtggc aatcgtagcc cagctcattc
    13261 agccgggttt cgaaatccgg ttcatggtcg ccaagttcga acgccgccag tacgcgcccg
    13321 tagtcggtgc catggctgcg atagtggaac aaagaaatgt tccagtgcgt acccagcgtg
    13381 ttgaggaagc gcagcagcgc gcccggtgat tccgggaatt cgaagctgta gaggcgttcc
    13441 tgcaacggat gtgacggacg cccgccgacc atatagcgca catgcaactt cgccatttcg
    13501 tcgtcggaga gatccaccac gctgtagccg ccgtcgttga gcatctgcaa aatttctttg
    13561 cgctcttcga ggccgcggct caggcgcacg ccgacaaaga tgcaggcgtt tttggcatcg
    13621 gcaaaacggt agttgaactc ggtgaccgat cgcccgccaa gcagttggca gaatttgagg
    13681 aagctgcctt tttcttccgg aatggtcacc gccaacaacg cttcacgctg ttcgcccagt
    13741 tcgcagcgtt ccgagacgta gcgcaggccg tggaagttca cgttggcacc ggaaagaata
    13801 tgcgccagcc gttcgccgcg aatgttgtgc tgggcgatat attttttcat tcccgccagc
    13861 gccagcgcgc cagagggttc cgccaccgcg cgcacatctt cgaataaatc cttcatcgcc
    13921 gcacagatcg catcactatc gacggtgatg atgtcgtcga gatactcctg gcataaacgg
    13981 aaggtttcgt caccgatgcg ttttaccgct acgccttcag caaatagccc tacgcgcggc
    14041 agatcaaccg gatgacccgc atccagcgct gctttcaggc aggcggagtc ttccgcttct
    14101 acggcgatca ctttgatttg tggcatcagt tgtttgatca gcaccgcaac gcccgccgcc
    14161 agaccgccgc cgccgactgg cacaaatacg cggtcgagat gggcgtcctg ctggagcagt
    14221 tccagcgcca gcgtgccttg cccggcaatc accatcggat ggtcgaacgg cggcacccag
    14281 gtgaacccct gctgctgtga cagttcgatc gctttagctt tcgcttcgtc aaagttcgcg
    14341 ccatgaagca gcacttcgcc gccgaatcca cgtaccgcat cgactttgat gtcggcggtg
    14401 gcggttggca taacgatcag ggccttcacg cctaaccgcg cagaagaaaa cgcgacgccc
    14461 tgcgcgtggt tacccgcaga agcagtgatc acgccgtgcg ctttttgttc ttccgtcagg
    14521 cccgccatca tggcgtatgc gccgcgcagc ttaaagctgt gcactggctg gcgatcttcg
    14581 cgcttcacca gaatgacgtt atcaagacgc gacgacagtt tttccatttt ttgtagcggc
    14641 gtaacctgcg ccgcctcgta aaccggcgcg cgcagcactg ctcttaaata ttcggcacct
    14701 tccggagcac cggacagggg ttgcgagtca gccattatta accccccagt ttcgatttat
    14761 cgcgcaccgc acctttgtcg gcgctggttg ccaggctggc gtaggcgcgc agggcaaagg
    14821 aaacctgacg ttcacggttt ttcggcgtcc aggctttgtc acctcgagcg tcctgcgctt
    14881 cacgacgcgc cgccagttcg gcatcgctta cctgtaactg aatgccacgg ttcgggatgt
    14941 ctatagcgat caggtcacca tcttcaatca ggccaatgct gccgccgctt gccgcttccg
    15001 gtgagacgtg gccgatggaa agaccagagg tgccgccaga gaaacgaccg tcggtgatca
    15061 gcgcacaggc tttaccgaga cccattgatt tcaggaagct ggttgggtag agcatttcct
    15121 gcatgccagg gccgcctttc gggccttcat agcggatcac caccacatcg cccgcgacaa
    15181 ctttgccacc gaggatcgct tctaccgcgt cgtcctggct ttcgtacact ttcgccgggc
    15241 cggtgaattt gaggatgctg tcatcgacgc ccgcggtttt aacgatgcag ccgttttctg
    15301 cgaaattacc gtagagcacc gccaggccgc cgtctttgct gtaggcgtgt tccagcgagc
    15361 ggatacagcc attggcgcga tcgtcatcga gagaatccca acggcaatcc tgcgagaatg
    15421 cctgtgtggt acgaatgcct gcaggacctg cgcggaacat attttttacc gcgtcatcct
    15481 gggtcagcat aacgtcgtat tgttccagcg tttgcggcaa cgtcaggcca agtacgtttt
    15541 tcacatcacg gttcagtaac cccgcgcgat ccagttcgcc gagaataccg ataacaccac
    15601 cagcacggtg aacatcttcc atatggtatt tctgggtgct cggcgcaact ttacacagct
    15661 gtggaacctt gcgggaaagc ttatcgatat cactcatggt gaagtcgatt tccgcttcct
    15721 gcgccgccgc cagcaggtga agtacggtgt tagtcgatcc acccatcgcg atatccagcg
    15781 tcatggcgtt ttcaaacgcc gctttgctag caatattgcg cggcagtgca ctttcgtcgt
    15841 tttgctcgta gtaacgtttg gtcaattcaa caatgcgttt accggcattg aggaacaact
    15901 gcttacggtc ggcgtgggtt gccagcagcg agccgttgcc cggctgcgac agacccagcg
    15961 cttcggtcag gcagttcatt gagttagcgg taaacatccc ggagcaggag ccgcaggtcg
    16021 gacacgcgga acgttcaacc tgatcgctct gggagtcaga aactttcggg tctgcgccct
    16081 ggatcatcgc atcaaccaga tcgagtttga ttatctgatc ggacagtttg gttttcccgg
    16141 cctccatcgg gccgccggaa acaaagatca ccggaatatt caggcgcagg gaagccatca
    16201 gcatccccgg ggtgattttg tcgcagttag agatgcagac catggcgtcg gcgcagtggg
    16261 cgttgaccat atactcaacg gaatcagcga tcagttcgcg agatggcagt gaataaagca
    16321 tccccccgtg gcccatggca atcccatcat ccaccgcaat ggtgttgaac tctttggcaa
    16381 cgccgccagc cgcttcaatt tgttcggcga ccagtttacc gagatcgcgc agatggacgt
    16441 gacccggtac aaattgggtg aacgagttca caaccgcgat aatcggcttg ccaaaatcgg
    16501 cgtcggtcat tccggtggcg cgccacagcg cacgagcacc cgccatatta cgaccatgag
    16561 tggtggtggc ggaacggtac ttaggcatac tttatttact cccagtgtct gtctcgtaaa
    16621 tgggacggtg cgtgccgtcc cattttttgt atttattgat taacttgatc taaccagccc
    16681 catttatctt cggtttcgcc agtgaagagg ccgaagaagg cttgctgaat gcgtttggta
    16741 accgggccac aacggccttc gccaacctga ataccatcta cactgcgcac tggcgtgatt
    16801 tctgccgcag taccggacat aaacacttca tccgccaggt acagggattc gcgcgacaga
    16861 acctgctcac gcacttcaat tcccagctct ttcgccagtt tgatgatggc atcacgggta
    16921 atacccggca gcgcggagga ggtgaacggt ggggtgaaca gcacaccatc tttcacttca
    16981 aacaggtttt cgcctgcgcc ttcagagata taaccgttca catccagcgc gataccttcc
    17041 tgataaccgt ggcggcgcgc ttcgctaccc accagcaggg aagagagata gttaccaccg
    17101 gcttttgccg cggttgggat ggtgtttggt gctgcgcggt tccaggagga aaccatcgca
    17161 tcgatcccct gctccagcgc ttctgcgccc agatacgctc cccacgggaa agcggcgata
    17221 atcacatcgg ttgagtatcc cgctggcggg tttacaccca tgccaacatc accaacgaag
    17281 atcagcggac ggatataggc gctggtgaga ttgtttttgc ggatcacgtc acgacaagct
    17341 tccatcagct catcaatgct ctgcgaaacc gggaagcgat agattttggc ggagtcatgc
    17401 agacgctgca tatgctcacg atggcggaat acaaccggtc ctttgtgcga gtcgtagcaa
    17461 cggatgcctt caaaaaccga agtgccatag tgcagcgcgt gcgacatcac atgcaccttc
    17521 gcgtcttccc agcgaaccat ctccccattg aaccaaatgt aatcagcttt cttcgtggtc
    17581 atttttatat tccttttgcg ctcaggcgcg gatttgttgt gatgtggttg tgctctggca
    17641 gatggcaacg tgtgcgacgt ccaccagttt atttaactga ctaaacagta agtcgaccga
    17701 ccgtgggctg gcaacggtca attcgatatt tatattttgt gcatcgctgg cggcagccat
    17761 attcattgag cagacgtgga aaccacgatg acgcaccacg cgtaaaacac gttctaaggt
    17821 ttccggattg aagcgagccg atacattgac ctgatgttgc atcatgataa tttctccaac
    17881 atttctgaat tactggcgcc aggcggcacc agcggccaga cgttctcaag ttcgtcgatt
    17941 gagacatgaa gcaggtatgg cccatcactg ttcagcatgg tgtcgagtgc cgcttcaacc
    18001 tggtctttac gggtgatgtg ttggccaggg atgccgaagg cgctggctaa catgaggaaa
    18061 tcggggttat cagtaagggt ggtttcgctg tatcgttcct gaaaaaacag ttgctgccat
    18121 tgtcgaacca tccctaaccg ttggttatcg agtaagacga ttttcaacgg taactgcttg
    18181 cgttttacgg tgcccagctc ttgcacattc atcatgaaag agccgtcacc ggagatacag
    18241 acgacagtat cgttcggtcg tgcgacttgt gcgccaaccg ccgctggtaa accgaaaccc
    18301 atggtgccta agccgctgga ggtaatgaaa ttttccgggc gagtgtgtgc gatgtgctgc
    18361 gcggcccaca tctggtgctg ccccacatct gtggtcacga cgcaatccgc aggtttacga
    18421 tccgacagtt gttttaacaa caacggcgcg tagatagcgt caccgggatg gtcgtaacgc
    18481 caggtatgtt catcacgcag ctgcgcgcag tattgctgcc agtcattgat atttaacggc
    18541 tgctgtaatg ctggtaacag agcatttaaa tcaccttgta atgccacatg tgcctgacgc
    18601 agcttgttca tttctgccgg gtcgatatcc atatggataa cactggcgtg tggtgcgaag
    18661 gtgttcagtt tgccggtcac ccggtcatca aaacgtgcac ccacggcgat cagcaagtcg
    18721 cactcctgca ccgcgaagtt cgccgctttg gtgccatgca ttcccagcat gcccaggtag
    18781 tacggataat ctgcttcaac tgcgcccagc cctttcagcg tgcaggtggc aggcattttt
    18841 gtggtagcga gaaattctcg taaagcagga actgcctgcg ccatacccac gccaccacca
    18901 acgtacagca tcggtttttg cgcttttgcc agcatctggc gcgcttgctc aacttcggca
    18961 tgtgggaaag tcacttcgtt ttcaacggtg gtgaaccacg gttccaggtc accgctggct
    19021 agctggatat cttttgggat atcgaccaga accggaccag gacgacctga gctggcaacg
    19081 tcgaatgctt cagccataat gcgcggcaac tcttccagcg actgcaccag aaagctgtgc
    19141 ttggtacagg ctaacgacaa tcccaggaca tccacttcct gaaatgcgtc cgtgccgata
    19201 aacggtgcgg acacttgacc ggtgatggca acaacaggga tagaatctaa cagtgcgtcc
    19261 gcaagcccgg ttatcaggtt ggttgcgccc ggaccagacg tggcgataca tacgccagtt
    19321 ttgccggtag cacgggcata accgatagcc gccattgccg caccctgctc atgtcggcac
    19381 agcaagtgct ccacgccgcc gtcatacaat gcatcgtaaa ccggcataat tgcgccaccc
    19441 ggataaccga aaacggtgtt cacaccctgt gcccgcaacg catgtaccac ccactgtgcg
    19501 ccattcatag ttagttcccc gtcctgaatc ttgagaaaca gaattttgtg ctgttattca
    19561 ttgtctgctc ctcggttata tttttaaggt caaaaaaaac ccccggacct ttcggtgcgg
    19621 gggtcttagt tcgttaaggc ttgatctcta agcctttcct cgtccaagtg cagccccgca
    19681 cggtgggata ataatcacca ccacgctaat catgaccagg ctaatcactc gtagaagggc
    19741 tgtcattttg tcttttcttg catcttgttc gaaggaatgc ctaaagagtt accatagatt
    19801 ttgcgaatag cacaagatat tttttggccg atttttatga tgcgtttaat aatttc
//
LOCUS       DAZGLJ010000071        19848 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000071 DAZGLJ010000000
VERSION     DAZGLJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.071"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            176..847
                     /locus_tag="ACHK49_002248"
     CDS             176..847
                     /locus_tag="ACHK49_002248"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO9262892.1"
                     /translation="MDKVLDSALPSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            857..2053
                     /gene="yaiP"
                     /locus_tag="ACHK49_002249"
     CDS             857..2053
                     /gene="yaiP"
                     /locus_tag="ACHK49_002249"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO9262893.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2031..2612
                     /locus_tag="ACHK49_002250"
     CDS             2031..2612
                     /locus_tag="ACHK49_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO9262894.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2614..3387
                     /gene="yaiO"
                     /locus_tag="ACHK49_002251"
     CDS             2614..3387
                     /gene="yaiO"
                     /locus_tag="ACHK49_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO9262895.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQNFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3573..3848
                     /gene="frmR"
                     /locus_tag="ACHK49_002252"
     CDS             3573..3848
                     /gene="frmR"
                     /locus_tag="ACHK49_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO9262896.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSMEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3883..4992
                     /gene="frmA"
                     /locus_tag="ACHK49_002253"
     CDS             3883..4992
                     /gene="frmA"
                     /locus_tag="ACHK49_002253"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO9262897.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5085..5918
                     /gene="fghA"
                     /locus_tag="ACHK49_002254"
     CDS             5085..5918
                     /gene="fghA"
                     /locus_tag="ACHK49_002254"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO9262898.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6043..6582)
                     /gene="yaiL"
                     /locus_tag="ACHK49_002255"
     CDS             complement(6043..6582)
                     /gene="yaiL"
                     /locus_tag="ACHK49_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO9262899.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6684..7895)
                     /gene="mhpT"
                     /locus_tag="ACHK49_002256"
     CDS             complement(6684..7895)
                     /gene="mhpT"
                     /locus_tag="ACHK49_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO9262900.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGDKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8167..9180)
                     /gene="mhpE"
                     /locus_tag="ACHK49_002257"
     CDS             complement(8167..9180)
                     /gene="mhpE"
                     /locus_tag="ACHK49_002257"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO9262901.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9177..10127)
                     /gene="mhpF"
                     /locus_tag="ACHK49_002258"
     CDS             complement(9177..10127)
                     /gene="mhpF"
                     /locus_tag="ACHK49_002258"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO9262902.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ASARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10124..10933)
                     /gene="mhpD"
                     /locus_tag="ACHK49_002259"
     CDS             complement(10124..10933)
                     /gene="mhpD"
                     /locus_tag="ACHK49_002259"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO9262903.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10943..11809)
                     /gene="mhpC"
                     /locus_tag="ACHK49_002260"
     CDS             complement(10943..11809)
                     /gene="mhpC"
                     /locus_tag="ACHK49_002260"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO9262904.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHQENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11827..12771)
                     /gene="mhpB"
                     /locus_tag="ACHK49_002261"
     CDS             complement(11827..12771)
                     /gene="mhpB"
                     /locus_tag="ACHK49_002261"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO9262905.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12773..14437)
                     /gene="mhpA"
                     /locus_tag="ACHK49_002262"
     CDS             complement(12773..14437)
                     /gene="mhpA"
                     /locus_tag="ACHK49_002262"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO9262906.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDVL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14628..15461
                     /gene="mhpR"
                     /locus_tag="ACHK49_002263"
     CDS             14628..15461
                     /gene="mhpR"
                     /locus_tag="ACHK49_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO9262907.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQD
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15538..16620
                     /gene="lacI"
                     /locus_tag="ACHK49_002264"
     CDS             15538..16620
                     /gene="lacI"
                     /locus_tag="ACHK49_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO9262908.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLLINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16743..19817
                     /gene="lacZ"
                     /locus_tag="ACHK49_002265"
     CDS             16743..19817
                     /gene="lacZ"
                     /locus_tag="ACHK49_002265"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO9262909.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTFATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWLAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKYQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPGLPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
BASE COUNT         4657 a         5436 c         5180 g         4575 t
ORIGIN      
        1 acatatgaat tgcataaaaa cattacccag tcgaacctcc gggtctgaaa gcccttgctt
       61 ttcagttatt agcaacattt aacatttata aattaacatc tggctgcgcc agagattacg
      121 tctctgagtg catcgttgtg cttgtagtat aataaataaa atggagtgtc attaagtgga
      181 taaggtttta gattcagccc tcccttcttc ggcaaataaa agaaagggta tccttgctat
      241 tggcgcacat cctgacgata tagaattagg ctgtggcgca tcgcttgctc gtcttgcgca
      301 aaaaggaatt tatatcgcag ccgtggtgat gactaccggc aactctggta cagatggaat
      361 aatcgatcgc catgaagaat cgcgcaacgc cctaaagata ttagggtgcc accaaactat
      421 tcatcttaat tttgctgaca cccgcgctca tttacagctc aatgatatga tttccgctct
      481 ggaagacatc attaaaaatc aaattccttc tgatgttgaa atcatgcggg tatataccat
      541 gcatgatgcc gaccgccatc aggatcatct cgctgtttat caagcttcaa tggttgcctg
      601 ccgcactatt ccacaaattc tcggctacga aaccccaagt acctggcttt catttatgcc
      661 tcaggttttt gaatccgtta aagaagaata tttcacagtc aaacttgctg cattaaaaaa
      721 acataaaagc caggaacgac gtgattatat gcgtcatgat cgcctgcgtg cagttgcaca
      781 atttcgcggg caacaggtca atagcgatct gggtgaaggc tttgtaattc ataaaatgat
      841 tctttgagca aattgcatga aaacctggat atttatctgt atgtctatag caatgttgct
      901 atggttttta agtacgctaa gacgtaaacc cagtcaaaag aaaggctgta ttgacgccat
      961 tatacctgcg tataacgaag gcccgtgtct ggcgcagtca ctggataatc tactgcgtaa
     1021 cccttatttt tgccgggtaa tttgcgttaa cgacggctcc acggacaata ccgaagcggt
     1081 catggcggaa gtcaaacgca aatggggcga ccgctttgtt gccgtcacgc aaaaaaatac
     1141 cggtaaaggt ggtgcgctga tgaatggcct caattatgcc acctgcgacc aggttttttt
     1201 aagtgatgcc gacacctatg ttccgcccga tcaagacgga atgggctata tgctggcaga
     1261 aattgagcgc ggtgctgatg ccgtaggcgg cattccctct actgcgttga aaggcgcggg
     1321 gctgttaccg cacatccgcg cgaccgtaaa gttgccgatg attgttatga agcgcacgct
     1381 acagcagctc ctgggcggcg caccgtttat tatcagcggt gcctgcggaa tgttccgtac
     1441 tgatgtattg cgtaagttcg gtttctcgga tcgtactaaa gtcgaagacc ttgatctcac
     1501 ctggacatta gtggcaaacg gctaccgtat tcggcaggcg aatcgctgca tcgtataccc
     1561 acaggaatgc aacagcccgc gtgaggagtg gcgtcgctgg cggcgttgga ttgtgggata
     1621 cgcggtctgt atgcgcctgc ataaaagact tttatttagc cgcttcggta tcttcagtat
     1681 atttcctatg ctgttggttg tgctttatgg cgttgggatt tatctcacta cctggtttaa
     1741 tgaattcatc accaccgggc cgcatggtgt ggtgttggca atgtttccgc ttatctggat
     1801 cggcgtagtt tgtgttattg gtgcttttag cgcctggttt catcgttgct ggttgttggt
     1861 gcctttagcg ccgctttccg ttgtgtatgt attattagct tatgccatct ggattattta
     1921 tggacttatt gcctttttta ctggacgcga acctcagcgc gacaaaccca cccgctattc
     1981 cgcactggtg gaagcgtcaa ccgcttattc ccaaccttct gtcacaggaa ctgaaaaact
     2041 atctgaagct taatgttaaa gagaaaaata ttcagattgc agaccaggta attattgatg
     2101 aaactgcagg tgaagttgtt atcggcgcga atacccgtat ttgtcatggt gccgttattc
     2161 agggtccggt agtgattggc gcaaactgcc tgataggtaa ttatgcgttt attcgtcctg
     2221 gcacaataat cagcaatggt gtaaaaattg gttttgccac cgaaattaaa aatgcggtta
     2281 ttgaagcgga agcaacgatt ggtccgcaat gttttattgc cgactcggta gttgcaaacc
     2341 aggcatattt gggcgcacaa gtacgtacca gtaatcatcg tctggatgaa cagcctgtgt
     2401 ctgttcgaac tccagaggga attatcgcta ccggatgcga taaattaggt tgttatatcg
     2461 ggcagcgttc acgccttggc gtacaagtta taattttgcc tgggcgaatt atttccccga
     2521 acacgcaact tggcccgcgc gtgattgtag aacgtaattt acctgccgga acttattcac
     2581 tccgacaaga acttatccgt acaggagatt aaaatgataa aacggacgtt attagcggcg
     2641 gccattttta gcgcattgcc cgcttatgcc gggttaactt ccattaccgc gggctacgat
     2701 tttaccgatt attctggcga tcatggcaac cgtaatttag cgtatgctga actggtggcg
     2761 aaagttgaaa acgcaacgct gctttttaat ctttcccagg ggcgtcgtga ttatgaaact
     2821 gaacatttca atgccactcg cggtcagggt gcggtctggt ataaatggaa taactggctg
     2881 acaacccgaa cgggtattgc ctttgcggat aatacgccgg tctttgcccg ccagaatttt
     2941 cgtcaggata ttaacctggc cctgttgcca aaaacgcttt tcacgaccgg ttatcgctac
     3001 actaaatatt acgatgatgt cgaagtcgat gcctggcaag gcggcgtatc actctatact
     3061 ggcccggtga tcaccagcta ccgctatacc cattatgact ccagcgatgc aggtggtagt
     3121 tatagcaata tgatttccgt gcgcctgaat gacccgcgcg gcactggtta tacgcaacta
     3181 tggctaagcc gcggaacagg cgcttacacc tatgactgga cgccagaaac acgctacggc
     3241 agcatgaaga gcgtcagtct gcaacgtatt caaccgctga ctgagcaact taatctcggg
     3301 ctgacggcag gtaaagtgtg gtacgacacc ccaaccgatg attataacgg tctgcaactt
     3361 gcagcccatc tgacctggaa attctgattc cttctgccgc ccgctatccg gggcggcctt
     3421 ccctgccgat tagccccccc ctttcctctt tgttttccga ccacattcac cggataaatt
     3481 ttattctcca gtgttatata ctataggggg gtatgcattg acatgtagta taccccccta
     3541 tagtatattg cgtgcagatg atgaggtgcg aaatgcccag tactccggaa gagaagaaaa
     3601 aggtccttac tcgagttcgt cgtattcgag ggcagattga tgctctggaa cggtcgatgg
     3661 agggtgatgc cgaatgccgt gccatactcc aacagatcgc tgccgttcgg ggcgcggcta
     3721 acgggctgat ggcagaagtg cttgaaagcc atatccggga aacgtttgac cgaaatgact
     3781 gctacagccg cgaagtcagc caatccgttg acgacactat tgaactggtt cgagcctatc
     3841 ttaaatagct gaatctatta ccatattgag gaagagcgag agatgaaatc acgtgctgcc
     3901 gttgcatttg ctcccggtaa accgctggaa atcgttgaaa ttgacgttgc accaccgaaa
     3961 aaaggtgaag tgctgattaa agtcacccat accggcgttt gccataccga cgcatttacc
     4021 ctctccgggg atgacccgga aggtgtattc ccggtggttc tcggtcacga aggggccggc
     4081 gttgtggttg aagtcggtga aggcgtaacc agcgtcaaac ctggcgacca tgtgatcccg
     4141 ctttacaccg cggagtgcgg cgagtgtgag ttctgtcgtt ctggcaaaac taacctctgt
     4201 gttgcggttc gcgaaaccca gggtaaaggc ttgatgccag acggcaccac ccgtttttct
     4261 tacaacgggc agccgcttta tcactacatg gggtgctcta cattcagtga atacaccgtg
     4321 gtcgcggaag tgtctctggc caaaattaat ccagaagcaa accatgaaca cgtctgtctg
     4381 ctgggctgtg gcgtgaccac cggtattggc gcggtgcaca acacagctaa agtccagcca
     4441 ggtgattctg ttgccgtgtt tggtcttggc gcgattggtc tggcagtggt tcagggcgcg
     4501 cgtcaggcga aagcgggacg gattatcgct atcgatacca acccgaagaa attcgatctg
     4561 gcgcgtcgct tcggtgctac cgactgcatt aacccgaatg actacgacaa accgattaaa
     4621 gatgtcctgc tggatatcaa caaatggggt atcgaccata cctttgaatg catcggtaac
     4681 gtcaacgtga tgcgtgcggc gctggaaagt gcgcaccgcg gctggggtca gtcggtgatc
     4741 atcggggtgg caggttctgg tcaggaaatc tccacccgtc cattccagtt ggtcactggt
     4801 cgcgtatgga aaggttccgc gtttggcggc gtgaaaggtc gttcccagtt accgggtatg
     4861 gttgaagatg cgatgaaagg tgatatcgat ctggaaccgt ttgtcacgca taccatgagc
     4921 cttgatgaaa ttaatgacgc cttcgacctg atgcatgaag gcaaatccat tcgaaccgta
     4981 attcgttact gatttcccgc aggtataccc cgtccacttc agacggggtt cttaatactc
     5041 tccctgggca gccgtccggg ggattaaccg tgagataatg actgatggaa ctcattgaaa
     5101 aacatgccag ctttggcggc tggcaaaatg tgtatcggca ttattcccaa tcactgaaat
     5161 gtgaaatgaa tgtcggcgtc tatctcccac caaaagccgc gaatgaaaaa ttgccggtgc
     5221 tgtactggct ttcgggcctg acctgcaacg agcagaattt cattactaaa tcggggatgc
     5281 agcgttacgc ggctgaacac aacattattg ttgttgcgcc ggacaccagt ccgcgaggca
     5341 gtcatgtcgc agatgccgac cgttacgatc tcgggcaagg tgccgggttt tacctgaacg
     5401 caacgcaagc gccgtggaat gaacattaca aaatgtatga ctatatccgc aacgagctgc
     5461 cggatttagt gatgcaacat tttccggcaa cgaccagaaa gtctatctct ggtcattcta
     5521 tgggcgggct gggcgcgctg gtgctggcgt tacgtaaccc agatgaatat gtcagcgtct
     5581 cggcgttttc gcccattgtc tccccatcgc aagtaccgtg gggacagcaa gcctttgccg
     5641 catatcttgg tgaaaataaa gatgcctggt tgaattacga tccggtgagt cttatttcac
     5701 aaggtcaacg cgttgcggaa atcatggttg atcaggggtt gagtgatgat ttttacgcgg
     5761 aacagctgcg gactccaaat cttgaaaaga tctgccagga gatgaatatc aagacgttaa
     5821 tccgttatca cgagggttac gaccacagct attattttgt ctccagtttt attggcgagc
     5881 atattgccta ccacgccaat aaactgaata tgcgttgata atagtgcacg actgccggat
     5941 gcggcgtgaa cgccttatcc gcctacattt cgcccgcagg ccgtaggcat gataagacgc
     6001 gcatagcgtc gcatcaggca tctgcgcacg acttatctat gtttaccaca tcaaatcatc
     6061 aggcactttg aagtcggcat atgggtcgtc ttcatcctgc tcttcggcgc tgagcgcgct
     6121 gtgcaacact atactgctgg catcacgctg agctatttta tcggcaacgc tggcgggaat
     6181 aatggcgtat tcaccttcgc tattgttatc gaccaacaag cgagcaatcg ccagacgacc
     6241 attaatcaac tgcgcctgtg tgagcttatc gacaaaaatc ttcttaatca gattgccgtc
     6301 agtgaagtta aaaccaatat cgccattggc aatggtgatt cggttcattt caatgagctg
     6361 cttcacctga gctttatatt ctttcgccaa cgcagcttgt ttttgttgct cgctaagctg
     6421 tttatcacgc tcaagctgtg cctttttatt ttcttctacc gccgcccgag cttcacgcgc
     6481 ctgaacgcgc gattttttcg ccgttctctc caccttcgcc gcttttttgc tggtgactaa
     6541 tcctgctttg agcaactgct cttgtaaggt aagttttgcc atctttgttt ccaaacccgc
     6601 tgaataattt ttagcgagta tacctgtaaa cgccgtgggt gtattggggg caactccctt
     6661 tcgcccctgc ttaataaggc gcgtcaggca tcggcacacg gctgcattct tgatctccga
     6721 ctcatcaaaa taaacaccgc caacccagca acaagaatac ccggtgcaga agcggccatt
     6781 acgccgaccg tgccagtgcc taatgccagc attttcccgg ccagtaacgg accgctcata
     6841 gcccccagac gccctaccgc cacggctgtt cccacacctg ttgcgcggat ctgcgaactg
     6901 taaaacaacg gtgccagggc atacaaaacg ctttgcccac ctgtcgcaaa caaccccgcg
     6961 acaaatcccg ccagcaacat accgttaaat gacgacaccg ttccaagtgc cagcagcgaa
     7021 gctaacatgc cgctataaat cagtagcgac atggtcactg gacgcagctt atccatcaat
     7081 gcgcccaaca ttaacgtccc gcttgccgca cccatttgca gggcaaacat cacccctgcc
     7141 gcctgcgatg gctggaatcc ttgctccacc aaaagcagcg gtagccagtt gatcaacatg
     7201 tagaccacca gcagagtgaa gaaataacac aaccacagca gcagcgtcgc ggttgccgtt
     7261 tctggcgcaa ataaggcacg cagtggtggc gcagcctgtt tatcgccagc gaaaaccgcc
     7321 gactccggca gccagcgcat taatagcggc accagaatca acggcaccac accacctacc
     7381 caaaacaccg tttgccatgc taagtttgcc cccgcgaaac ccagtgtcgc cgccagcgcc
     7441 gcgccaatgg gaacaccgca atacatcagg ctcactgctg tcccacgaaa acgtggaccc
     7501 gcggcttcag acgtcagggc gataagattc ggcaacgccg cccccagccc gacaccggtc
     7561 atcagccgcg caaagaccag tgaggggaaa tcccaggcaa tcgccgttgc cagcgagaac
     7621 aaaccaaaca gcgcaactga gccaatcaaa atgcgcttgc gaccataacg gtccgccagc
     7681 attccgccaa ccaacgcgcc gggtagcaaa ccgagtattc cggcgctgaa tatccagccc
     7741 atttgcattt tatcgagtgc gaaagcctgg gcgatgccac ccgccgcaat gccagccgcc
     7801 tgaagatcca gcccttccat cagagcgacc aaaaaacaaa gcccgatagt cagcatcagg
     7861 cgggatgaag atgatgaagg ggtacgagtc gacataagat gaacctcgtt gcgagtgcag
     7921 gtgaatcgcg actgatacat tgccagccgc gtaatcagaa ctatttaatg actgcctacg
     7981 ggcgaaatgt aggccggaca agacgtttat cgcatccggc agtcgtgtgc agatgcctga
     8041 tgcgacgcta acgcgtctta tcaggccaaa ttctcacgcc acccgtaggc cggataaggt
     8101 gtttacgccg catccggcag tcgtgctggg atgggcatac aaaatgcacg ttctcggcag
     8161 tcatcattat ttgttgttgc gcagatccag cgccacgtca acgatcatat cctcctggcc
     8221 gccaaccatc cggcgtttgc ccagctcaac gagaatatcc accgcactta agccataacg
     8281 cgccgccgcc gtttcacagt gacgcaggaa gctcgagtaa acaccagcgt atcccagcgc
     8341 cagcgtttcg cgatcgactc gtaccggtcg atcctgcaac ggacgcacca ggtcgtcagc
     8401 ggcatccatt aacgcataga gatcggtccc atgctgccag cccagtttat ccgcggcggc
     8461 aataaatact tccagcggcg cgttacctgc gcccgcgccc attcccgcga ggctggcgtc
     8521 gattcggtcg cagccctctt ccaccgccgc gatagagttc gccacgccaa gactcaggtt
     8581 atggtgagcg tgcatgccag tttgcgtttc tggtttcagc tctgctttca gggcgcggaa
     8641 acggtcacgg atatcgctca tgttcatcgc accgccagaa tccaccacat aaatacaggt
     8701 cgcaccgtag ccttccatca gctttgcctg ctttgcgaga ttctccggcg tggtcatatg
     8761 gctcatcatc agaaaaccaa cggtgtccat tccgagctcg cgggcatact gaatatgctg
     8821 ggcggaaaca tcagcttcgg tacagtgcgt tgccacacga accacccgcg cgccagcctg
     8881 ccaggcattt ttcagatcgt gaatagtgcc gattcctggc agcaacaacg tcgcgatttt
     8941 ggcgtgcttc accacatccg ccgctgcttc aatccattca aggtcgctat gtgcgccgaa
     9001 accatagtta aagctggaac cctgcaaacc gtcgccatgg gccacttcaa tcgaatccac
     9061 gcgggcatcg tccagtgctt tggcaatctg gcgaacgttt tccagcgaat actgatgacg
     9121 aatggcgtgc ataccgtcac gcaacgtgac gtccgagata taaagttttt taccgttcat
     9181 gccgcttctc ctgccttgcg cgccagtgac tgggccattt tttccgctgt cgccagcgca
     9241 ctggaagtca taatgtcgag gttgcccgca taggcaggca gataatgcgc tgcgccttcg
     9301 acttccagcc agaccgctgt tttcagtccg gagaattgcc ccacgcccgg taaattgacc
     9361 ggtttatcct gcgggataac ttcaaactgc acgcgctgtt tcaggcgata acccggtacg
     9421 taagcctgca ccgcctcagc catttcattg attgaggctt cgatatcatc ttgtgaagct
     9481 tcgtcgctca atacatacac cgtgtcacgc atcatcaacg gtggctctgc tgggttaagc
     9541 acaataatcg ccttcccttt tgccgcgccg cccaccactt caatggctcg ggaagtggtt
     9601 tccgtaaatt catcgatatt ggcacgcgtg ccaggtccgg cagatttact ggcgatagaa
     9661 gcgataattt cggcgtaatg aacacgcgcc acgcgtgaaa ctgccgccac cattggaatg
     9721 gtggcctggc cgccgcaggt gaccatgttg acgttcagtt gatcgacgtt cgcctcgagg
     9781 ttaaccaccg gcacgcagta agggccgatg gcagcaggcg tcaggtcaat taagcgaata
     9841 tccggtttcg cttcgcgtaa agcggcatcg tttttcacat gcgcgcccgc gctggtcgca
     9901 tcaaatacaa tgtcgatatc agcaaattca ggcatgttca tcagcccgat caccccttca
     9961 tgggtggtgg cgacgcccat acgtctggca ctcgccagac cgtcggactg aggatcaatg
    10021 ccgaccatca ccgccatctc cagatgctga ccgtgacgca aaattttaat catcagatcg
    10081 gtgccaatgt tgccagaacc gataatggcg actttacgct tactcatgac agacttcctt
    10141 ttggggctgc gcttgaaaat gtcgcagcaa ctgaacctat gccttcaata tgggcttcaa
    10201 aacgatcgcc cgcattcacc gccaccatcg ggcctaatgc cccggtaaga atgatatctc
    10261 cggcgcgcag cggttcaccc agactggcca ttttgcgtgc cagccagacg gcggcattaa
    10321 gcggatgtcc caggcattcg ctgccgcgcc cgctggaaac ctcttcgtta ttacgcgtca
    10381 tcttcatggc gcagtttttc aggtctaacc ccgccggacg ttgcgccgga ccgccgatga
    10441 catacacccc gcaggaagcg ttatctgcca cggtatcgac aaactgaatc gaccagtcgc
    10501 gaatgcggct ccccaccact tccagcgccg gaagtaccca ttcaatggcg ttatacagtt
    10561 cgtcgaaggt gatatcagtt gcgggtaaat cgcggttcaa caccagtgcg atctccgctt
    10621 caatgcgggg ttgcagaaca cgggaaaaag gaatgatttc gttatcgcca taacacatgt
    10681 cggcaaataa cgtcccaaaa tccggttgat caacgcccag ttgttgttgc actttcggat
    10741 gcgtcaggcc cactttacgc cctaccacgc gacgcccctg cacaacgtca tattgcacat
    10801 ttatgtgctg aatggcgtag gccgcttcag cgttatcgat accaatcaga tcgcgcagcg
    10861 gcgcaatcgc ttcgccctgc tctgcggcgc ggcgtaaatc cgccgccagt tgctcaagag
    10921 tatgcttcgt catgaccatt ccttaagggc gtgcgaggaa attcagcacc aattgattga
    10981 aagcgtcggc atgttcccac tgcgcccagt gaccacagtc gcggaagata tgcagttcag
    11041 aaccggcaat gccggacagc agacgcagac ccgcatccat cggcacaaag cggtcgttgc
    11101 gcccccagac aatcagggtt tgcgctttga tttccgccag acgtgggcca aaatccggga
    11161 actgtttcgg attagcttcc aggctcttaa cgaagttttc ctggtgatcg cggcgcgaca
    11221 gcatattatt caggcgcgct tcaaacaggg cgtcggtcaa atcgctggta tcgaaaacga
    11281 agatatccat catcagcttc aggttttcga tagtcggctg acgataaagc tgattcagtc
    11341 gcttaatacc ttcggttggc atcggcgtaa acaaactcat gccgcccgta ccgccgccca
    11401 tcagcaccag tttgccgacg cgctccggcc agttaagggt gaacgccaca gaactatggc
    11461 cgcccatcga gttgcccagc aggtggattt tggcgatatc cagttgatcc accacgcttt
    11521 tcaggattcg tgcattaaga tccgatcgtg aaccactatt aacgatcgaa tcgcttttgc
    11581 cccaacccgg acaatccagc aggattaccc gatagcccgc ctctaccagc ggatcgatat
    11641 tgcggctgaa gttcgcccag ccagtagcac ccgggccgga accatgcagc aggacaacgg
    11701 tttcgtcgcc ttgtccgcag tcattaaaat ggatgcgcag cgttttaccc gcttcttcta
    11761 cattcagaaa acggctggtg gcggcttcgg tttgtggctg ataactcatc atcttctcct
    11821 gcatattcag ttctctgttc tggcgcttaa cgagccaaat ccggcaatcc actccgggat
    11881 tgggcggtaa taacgccctt cgctacgcca gttgccaaat gtagaaatag cggcaaaagc
    11941 ggcgacccag gttttgattt catgtgtcga ctttccggca atggcggaaa gctcttcgtt
    12001 actgacggca tccagttcct gtatgcgtcc ctgctccagc aaagtcatga actggttatc
    12061 ccaaatcggg ttgagcggat gcagagttct ctgatcctca acaaacttct cagcggcgct
    12121 aatcacccgt tgctgacgca attcgcgctc actggcgggt aaatctttcc cgctccccaa
    12181 cagacggtcg cgcatatggg catcggcttt cgccagttcg ggcaccggcg gctgatggga
    12241 aagcccaccg gaacccagga acagcacgcg tttattgaga gtgctggtga aacgtccaat
    12301 ggcttcaccc aacatgcggg tacgctggaa accgggcagc ggcgtggcga caccgttgat
    12361 gaacacaggc agaactggca ccttatccag cccaccgagc aggaactcca gcggctgggc
    12421 gaacccgtgg tccacctgca tacagtaaga aacggcaaga tcgatcccgc tcttcatgac
    12481 agcatgcgca caggcctccg ccagctccac aggcacgggc agctctcctg ccgcactgcc
    12541 gaaatcacca attgccgtcg ctccaacgcc taaacagaac ggtggcatca cgtcatagaa
    12601 aaagccgttg tagtgatctg gcgcaaacag caccaccagt tcaggggaga aagccgcaat
    12661 acgctcgcgg gcgctggcaa tcacgccatt gacctcatcg agtacctctt gcgccgggtc
    12721 gacatacccc accaacggcg agtgggaaag acagtgaaga taagcgtgca tatcaggcta
    12781 ccttttcgac agaaacgtcg gcatcagggc gggtcagcgt catcaccgac gccagtttat
    12841 tcagcgtatt gcccagggtt tgcggaatgg cggtggcggc aacaaagcga tccgggcgca
    12901 tcaccaccag cgaagcattg tgttgcgcga accagctacg cagacgacct tgcgtatcgc
    12961 ccacgcgtag cacgccgtcg tggttatcct gtgcggtatg aatttgcact tccggcacca
    13021 cctgaatgaa gcgtgtgccc aacgcgcgcc actgctggat ttgctcgtcg ctcatccccc
    13081 acagtggatt gcatccccag ccaattaccg cgaagttcgc gccaatcgcg ttatcgagca
    13141 gcgtcacgtc gccgttttcc agcgtgactt tcggctgaat aaacatcttg ccgaccggag
    13201 agtgcttcgc ttcgccctca cgtaccagcg caccgccgta atattgcggc atcggcttga
    13261 agcgcatttc gaggaagtag cgttttactg gcggcagata attcagtagc caggaaacgc
    13321 cgtcacgtaa cgtaccctgc cagcgtttcg gcggagccag cacgttgccc gccgtcacgg
    13381 acaggtcaat catcgctttg gcgtgatcgc gacgttcttg ttgataggta tcgagcagca
    13441 catcgcgggc tttcccctgg ataaccaacg ccagtttcca ggcgaggtta aaggcgtcgc
    13501 gcataccact gttatagccc tgcccctgcc acaccggcat gatgtgcgcg gcatcgcccg
    13561 ccagcagtac gcgatcaata cggaaacgtt gcgccagtcg cgcgttatgg gtgtagacac
    13621 gctggcgaat caattcaaca ttgtccggat taggcagcac tttgcttaac agcttgcgca
    13681 tattttgcgg ctcacgcagc tgctcttcgg tttctcccgg catcaccata aattcaaagc
    13741 gacgtaccgc atgaggcagc gcggcagaaa catacgggcg caccggatcg caacacaaat
    13801 agatatgcgg cgtacttaac ggatcgttgg cgatatctac cacaatccac tgatttggcg
    13861 cagttttacc ttcaaacggc acattcagag tgcgacggac aaaacttgct ccaccgtcac
    13921 aggctaccag ccactgggct ttgactattt cccgctgccc ttctgccgtt ttcaggtgca
    13981 aggtcacttc gtcattttgc tggctgaagg cctccagctc gcgggaaaac aagcagcgca
    14041 cattaggaaa acgcgacacc ccttccagca tcaccgcatc gacctgcggc tgaataaagg
    14101 cgttacggcg cggccagcca aattcatcgg tcattggctg aatatcagca aaacagcggc
    14161 ctttcggggt gagaaaacgc atcgcgtgcc acggcgtagt gtgcggcaga acattctcga
    14221 ccaggccaac cgactgcatg gtgcgcagcg cctcgtcatc aataccaatc gcacgcgggt
    14281 agtcgatcaa cttatcgagt ttctccacca ccagcacgtc aatgcccatc tgaccgagat
    14341 agttcgccat catcagcccg accggaccgg cacctgcgat cgccacctga acgctatggt
    14401 taacagcagg ctggatgtca gggtgttgta ttgccatttc agtacctcac gactcggaca
    14461 aaatgccgtt gcgcgcacag tacggcgcaa cttattttgt taaaaacatg taaatgattt
    14521 tttattgtgc gctcagtata ggaagggtgt tttcggctac aatcaaaaca tgcccgaatg
    14581 tgcaccaggt gcacaacgtt gttttaacta tagaaatgtc aattaatatg cagaacaatg
    14641 agcagacgga atacaaaacc gtgcgcggct taacccgcgg tctaatgtta ttaaatatgt
    14701 taaataaact tgatggcggt gccagcgtcg ggctgctggc ggaactcagc ggcctgcatc
    14761 gcaccactgt gcggcgactg ctggagacgc tgcaggaaga gggatatgtc cgccgtagcc
    14821 cctccgatga tagttttcga ctgaccatca aagtgcggca attaagcgaa ggatttcgtg
    14881 acgaacagtg gatttctgca ctggcggccc cactgctggg cgatctgttg cgcgaagtgg
    14941 tatggccgac agatgtgtcc acgctggatg ttgatgcaat ggtggtacgc gaaaccactc
    15001 accgtttcag ccgcttatcc tttcaccggg caatggtcgg gcgacgtttg ccgcttctga
    15061 aaaccgcctc gggcctgacc tggctggcct tttgcccgga acaagaccgc aaggaattaa
    15121 tcgaaatgtt agcctcccgc cccggtgatg actatcaact ggcacgggaa ccgttaaagc
    15181 tggaagccat tctggcgcgc gcgcgcaaag agggttacgg acagaactac cgcggctggg
    15241 atcaggagga gaagatcgcc tctatcgccg taccgctgcg cagtgaacaa cgggtgattg
    15301 gctgtctgaa tctggtgtat atggcgagcg caatgaccat tgaacaggca gcggaaaagc
    15361 atcttccggc gctacaacgg gtagcaaaac agatcgaaga aggggttgaa tcgcaggcta
    15421 ttctggtggc cggacggcga agcggcgtgc atttacgttg acaccatcga atggcgcaaa
    15481 acctttcgcg gtatggcatg atagcgttcg gaagagagtc aattcagggt ggtgaatgtg
    15541 aaaccagtaa cgctatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc
    15601 cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg
    15661 atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg
    15721 ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg
    15781 gcgattaaat ctcgcgctga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga
    15841 agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg
    15901 ctgctcatta actatccgct ggatgaccag gatgccactg ctgtggaagc agcctgcgct
    15961 aatgttccgg cgttatttct tgatgtctct gaccagactc ccatcaacag tattattttc
    16021 tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa
    16081 atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg
    16141 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt
    16201 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg
    16261 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg
    16321 ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt
    16381 tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
    16441 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc
    16501 tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg
    16561 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
    16621 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
    16681 gcttccggct cgtatgttgt gtgaaattgt gagcggataa caatttcaca caggaaacag
    16741 ctatgaccat gattacggat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
    16801 ctggcgttac acaacttaat cgccttgcgg cacatccccc tttcgccagc tggcgtaata
    16861 gcgaagaagc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
    16921 gctttgcctg gtttccggca ccagaagcgg tgccggaaag ctggctggag tgcgatcttc
    16981 ctgaggccga tactgtcgtc gtcccctcaa actggcagat gcacggttac gatgcgccca
    17041 tctacaccaa cgtgacctat cccattacgg tcaatccgcc gtttgttccc acggagaatc
    17101 cgacgggttg ttactcgctc acatttaatg ttgatgaaag ctggctacag gaaggccaga
    17161 cgcgaattat ttttgatggc gttaactcgg cgtttcatct gtggtgcaac ggtcgctggg
    17221 tcggttacgg tcaggacagt cgtttgccgt ctgaatttga cctgagcgca tttttacgcg
    17281 ccggagaaaa ccgcctcgcg gtgatggtgc tgcgctggag tgacggcagt tatctggaag
    17341 atcaggatat gtggcggatg agcggcattt tccgtgacgt ctcgttgctg cataaaccga
    17401 ctacacaaat cagcgatttc catgttgcca ctcgctttaa tgatgatttc agccgcgctg
    17461 tactggaggc tgaagttcag atgtgcggcg agttgcgtga ctacctacgg gtaacagttt
    17521 ctttatggca gggtgaaacg caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta
    17581 tcgatgagcg tggtagttat gccgatcgcg tcacactacg tctgaacgtc gaaaacccga
    17641 aactgtggag cgccgaaatc ccgaatctct atcgtgcggt ggttgaactg cacaccgccg
    17701 acggcacgct gattgaagca gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa
    17761 atggtctgct gctgctgaac ggcaagccgt tgctgattcg aggcgttaac cgtcacgagc
    17821 atcatcctct gcatggtcag gtcatggatg agcagacgat ggtgcaggat atcctgctga
    17881 tgaagcagaa caactttaac gccgtgcgct gttcgcatta tccgaaccat ccgctgtggt
    17941 acacgctgtg cgaccgctac ggcctgtatg tggtggatga agccaatatt gaaacccacg
    18001 gcatggtgcc aatgaatcgt ctgaccgatg atccgcgctg gctaccggcg atgagcgaac
    18061 gcgtaacgcg aatggtgcag cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg
    18121 ggaatgagtc aggccacggc gctaatcacg acgcgctgta tcgctggatc aaatctgtcg
    18181 atccttcccg cccggtgcag tatgaaggcg gcggagccga caccttcgca accgatatta
    18241 tttgcccgat gtacgcgcgc gtggatgaag accaaccctt cccggcggtg ccgaaatggt
    18301 ccatcaaaaa atggctttcg ctgcctggag aactgcgccc actgatcctt tgcgaatatg
    18361 cccacgcaat gggtaacagt cttggcggtt tcgctaaata ctggcaggcg tttcgtcagt
    18421 atccccgttt acagggcggc ttcgtctggg actgggtgga tcagtcgctg attaaatatg
    18481 atgaaaacgg caacccgtgg ttggcttacg gcggtgattt tggcgatacg ccgaacgatc
    18541 gccagttctg catgaacggt ctggtctttg ccgaccgcac gccgcatccg gcgctgacgg
    18601 aagcaaaata ccagcagcag tttttccagt tccgtttatc cgggcaaacc atcgaagtga
    18661 ccagcgaata cctgttccgt catagcgata acgagctcct gcactggatg gtggcgctgg
    18721 atggcaagcc gctggcaagc ggtgaagtgc ctctggatgt cgctccacaa ggtaaacagt
    18781 tgattgaact gcctggacta ccgcagccgg agagcgccgg acaactctgg cttacggtac
    18841 acgtagtgca accgaacgcg accgcatggt cagaagccgg acacatcagc gcctggcagc
    18901 agtggcgtct ggcggaaaac ctcagcgtga cactccccgc cgcgtcccac gccatcccgc
    18961 atctgaccac cagcgaaatg gatttttgca tcgagctggg taataagcgt tggcaattta
    19021 accgtcagtc aggccttctt tcacagatgt ggattggcga tgaaaaacaa ctgctgacgc
    19081 cgctgcgcga tcagttcacc cgtgcaccgc tggataacga cattggcgta agtgaagcga
    19141 cccgcattga ccctaacgcc tgggtcgaac gctggaaggc ggcgggccat taccaggccg
    19201 aagcagcgtt gttgcagtgc tcggcagata cacttgccga cgcggtgctg attacgaccg
    19261 ctcacgcgtg gcagcatcag ggaaaaacct tatttatcag ccggaaaacc taccggattg
    19321 atggtagtgg tcaaatggcg attaccgttg atgttgaagt agcgagcgat acaccgcatc
    19381 cggcacggat tggcctgacc tgccagctgg cgcaggtcgc agagcgggta aactggctcg
    19441 gattagggcc gcaagaaaac tatcccgacc gccttactgc ggcctgtttt gaccgctggg
    19501 atctgccatt gtcagacatg tataccccgt acgtcttccc gagcgaaaac ggtctgcgct
    19561 gcgggacgcg cgaattgaat tatggcccac accagtggcg cggcgacttc cagttcaaca
    19621 tcagccgcta cagtcaacag caactgatgg aaaccagcca tcgccatctg ctgcacgcgg
    19681 aagaaggcac atggctgaat atcgacggtt tccatatggg gattggtggc gacgactcct
    19741 ggagcccgtc agtgtcggcg gaattccagc tgagcgccgg tcgctaccat taccagttgg
    19801 tctggtgtca aaaataataa taaccgggca ggccatgtct gcccgtat
//
LOCUS       DAZGLJ010000072        19556 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000072 DAZGLJ010000000
VERSION     DAZGLJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.072"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..175
                     /locus_tag="ACHK49_002266"
     CDS             <1..175
                     /locus_tag="ACHK49_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9262910.1"
                     /translation="DSSSEELLSDNTLEKDENSPDNGFEVGEYNTHEAYNSENQYFTR
                     EDYTYNYDLLNAI"
     gene            complement(570..946)
                     /gene="rnpB"
                     /locus_tag="ACHK49_002267"
     ncRNA           complement(570..946)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHK49_002267"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(979..2095)
                     /gene="garK"
                     /locus_tag="ACHK49_002268"
                     /pseudo
     CDS             complement(979..2095)
                     /gene="garK"
                     /locus_tag="ACHK49_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
     gene            complement(2192..3082)
                     /gene="garR"
                     /locus_tag="ACHK49_002269"
     CDS             complement(2192..3082)
                     /gene="garR"
                     /locus_tag="ACHK49_002269"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO9262911.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3112..3882)
                     /gene="garL"
                     /locus_tag="ACHK49_002270"
     CDS             complement(3112..3882)
                     /gene="garL"
                     /locus_tag="ACHK49_002270"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO9262912.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3898..5232)
                     /gene="garP"
                     /locus_tag="ACHK49_002271"
     CDS             complement(3898..5232)
                     /gene="garP"
                     /locus_tag="ACHK49_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO9262913.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5607..7178
                     /gene="garD"
                     /locus_tag="ACHK49_002272"
     CDS             5607..7178
                     /gene="garD"
                     /locus_tag="ACHK49_002272"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO9262914.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWLDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDIASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7327..7662
                     /gene="prlF"
                     /locus_tag="ACHK49_002273"
     CDS             7327..7662
                     /gene="prlF"
                     /locus_tag="ACHK49_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO9262915.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7662..8126
                     /gene="yhaV"
                     /locus_tag="ACHK49_002274"
     CDS             7662..8126
                     /gene="yhaV"
                     /locus_tag="ACHK49_002274"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO9262916.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(8181..8990)
                     /gene="agaR"
                     /locus_tag="ACHK49_002275"
     CDS             complement(8181..8990)
                     /gene="agaR"
                     /locus_tag="ACHK49_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO9262917.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVVERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9239..10519
                     /gene="kbaZ"
                     /locus_tag="ACHK49_002276"
     CDS             9239..10519
                     /gene="kbaZ"
                     /locus_tag="ACHK49_002276"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO9262918.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCHDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10542..11015
                     /gene="agaV"
                     /locus_tag="ACHK49_002277"
     CDS             10542..11015
                     /gene="agaV"
                     /locus_tag="ACHK49_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO9262919.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            11026..11805
                     /gene="agaW"
                     /locus_tag="ACHK49_002278"
     CDS             11026..11805
                     /gene="agaW"
                     /locus_tag="ACHK49_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO9262920.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11795..12673
                     /gene="agaE"
                     /locus_tag="ACHK49_002279"
     CDS             11795..12673
                     /gene="agaE"
                     /locus_tag="ACHK49_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO9262921.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFILLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12691..13125
                     /gene="agaF"
                     /locus_tag="ACHK49_002280"
     CDS             12691..13125
                     /gene="agaF"
                     /locus_tag="ACHK49_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO9262922.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLALQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            13122..14255
                     /gene="nagA"
                     /locus_tag="ACHK49_002281"
     CDS             13122..14255
                     /gene="nagA"
                     /locus_tag="ACHK49_002281"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO9262923.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            14606..15760
                     /gene="agaS"
                     /locus_tag="ACHK49_002282"
     CDS             14606..15760
                     /gene="agaS"
                     /locus_tag="ACHK49_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO9262924.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15773..16633
                     /gene="kbaY"
                     /locus_tag="ACHK49_002283"
     CDS             15773..16633
                     /gene="kbaY"
                     /locus_tag="ACHK49_002283"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO9262925.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16800..17276
                     /gene="agaB"
                     /locus_tag="ACHK49_002284"
     CDS             16800..17276
                     /gene="agaB"
                     /locus_tag="ACHK49_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO9262926.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            17315..18118
                     /gene="agaC"
                     /locus_tag="ACHK49_002285"
     CDS             17315..18118
                     /gene="agaC"
                     /locus_tag="ACHK49_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO9262927.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            18108..18899
                     /gene="agaD"
                     /locus_tag="ACHK49_002286"
     CDS             18108..18899
                     /gene="agaD"
                     /locus_tag="ACHK49_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO9262928.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18900..>19556
                     /locus_tag="ACHK49_002287"
     CDS             18900..>19556
                     /locus_tag="ACHK49_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO9262929.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQLLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     Q"
BASE COUNT         4752 a         5094 c         4978 g         4732 t
ORIGIN      
        1 agactctagc tcagaagagt tactttcaga taacaccctc gagaaagacg aaaattctcc
       61 ggacaatggt tttgaggtcg gggaatataa cacacatgaa gcatataact cagagaatca
      121 atattttacc agagaggact atacgtataa ttacgacctt ttaaatgcaa tatagactta
      181 ataacttctt acaaagtcta ttattaatac cgtagatatt tattcatctt accctgtgca
      241 ccaaaagcaa aaactcactg acgcgggttt gaaactgatg gcaaccgcaa aaatgcctga
      301 tgcgctacgc ttatcaggcc tacgccatct ctgcaatata ttgaatttgc gtgcttttgt
      361 aggcaggata aggcgttcac gccgcatccg gcatgaataa agcgcactca acaatctgaa
      421 acccgccgga acggtttatt acgtacatca ggtgaaactg accgataagc cgctttcttt
      481 tgggtatagc gtcgtggaca gtcattcatc tttctgcccc tccaaaagca aaaacccgcc
      541 ggtttacgct ggcgggtttt cgttaaatca ggtgaaactg accgataagc cgggttctgt
      601 cgtggacagt cattcatcta ggccagcaat cgctcactgg ctcaagcagc ctacccgggt
      661 tcagtacggg ccgtacctta tgaaccccta tttggccttg ctccgggtgg agtttaccgt
      721 gccacggact gttaccagcc gcgcggtgcg ctcttaccgc accctttcac ccttacctga
      781 tcccgcttgc gcgggccatc ggcggtttgc tctctgttgc actggtcgtg ggtttccccc
      841 ccaggcgtta cctggcaccc tgccctatgg agcccggact ttcctcccct ccgcccgtct
      901 cccccgaaga ggacgacgac gaagcggcga ctgtctggtc agcttcggcg cgcagtatag
      961 agggtttgcg cgcccttgtc accccgcgtt gcgcattcca atcgccagtg tcgcggcgat
     1021 attacgtgaa gcacggcaga tattgtcata agccccgcgg aatgcttcgt ccaacgtacc
     1081 aatgctggtc agtacgctga agacagcatc aatgccatgc tgatgtacaa cgccaacatc
     1141 atcggtcagg ctacccgcaa tgccaatcac cggtttatgg tacttcttcg ccacgtttgc
     1201 gacgccaatc ggtaccttgc cgtgaatact ctggctgtca atacgccctt caccggtgat
     1261 caccagcgta caatcgtgaa tatgctcctc cagattcagc gccgtagtga cgatttcaat
     1321 accacttttc agttccgcac caagaaacgc cattagcgcc ggggacatct ttcacatcaa
     1381 catgcagcgc ttttttaatg acctcggcat agtgagagag gttattgtcc agctcaacaa
     1441 tcatcgcttc actggctccc ttttgtgggc caaagatgcg cgatgcgccg ttatcgccca
     1501 ccagcggatt ggtgacatca caagcgacgc gaatgacgca atcttttaag cgcggatcga
     1561 ggccggaaat atcaatatca ttcagagtat taagactacc gccgccaaaa ccaatttcat
     1621 tgccgttggc gtcgcataat ttcgccccca gcgcctgtac catgcctgcg ccgccatcat
     1681 ttgtagcgct accgccaatg ccgataataa tgtttgtcgc gccgctctcc agcgcctgca
     1741 ggattaactc gcctgtgccg cgtgaagtgg tcacgagtgg atcgcgtttt tccgcaggta
     1801 ccagctccag cccactggcc gccgccattt caataaacgc ggttttgcca tcgccggaga
     1861 tcccccaact ggcattcact ttctcgccca gcggccctgt aacccaggcg tgacgttcag
     1921 aaccctgggt ggctgcaatc atcgcttcca ccgttccttc gccaccgtcg gcaaccggaa
     1981 tagaaacgta ctgtgcatca ggaaaaattt cccgaaatcc tttttctatc gcctgcgcaa
     2041 cctcgctggc agataaactt tctttataag agtctggggc gattacgatt ttcataccta
     2101 tgcctgttac cacatgacgc cggagggcgt ttctcttatt cggcctggat tccaggcccg
     2161 gattgcaata cgccatccgg gcacgacgtc attaacgagt aacttcgact ttcgccagtt
     2221 tttcgtagta gcacgccagg gcgctatgat ccgccgttcc taaaccatct gctcgcagtg
     2281 cctgcatcat ctccataacc gcagctgtga gcggcagttg cgcgccgacg ccgtgggaag
     2341 tatccagcgc atttgccaga tccttaatat gcagatcaat acggaagccc ggcttgaagt
     2401 tgcggtccat caccatcggc gctttggcat ccagcacggt actgcccgcc agtccaccgc
     2461 gaattgcctg atacaccagg tccgggttaa cgcccgcttt ggttgccagc gttagcgctt
     2521 ctgacatcgc ggcaatattc agcgccacaa tgacctgatt tgccagtttg gtgacgttac
     2581 ctgcaccgat ttccccggta tgcaccacgg aacccgccat cgctttcatc aaatcatagt
     2641 atttgtcgaa aatagccttg tcgccgccca ccatcactga cagcgtaccg tcgatggctt
     2701 tcggttcacc gccgctcacc ggagcatcca gcatatcaat gcctttcgct ttcagcgctt
     2761 cgctgatttc acggcttgcc agcggtgcga tagaactcat atcgatcaat accgtacctg
     2821 gcttcgcgcc ttcaataatg ccattctcac ccagcgccac ctctttcaca tgaggggagt
     2881 ttggcagcat ggttatgatg acgtcgcact gttcagcgat cgctttagcc gtagacgctg
     2941 tttctgcacc tgcagcaatc acgtcagcaa tagcttctgg gttacggtca gcaaccacca
     3001 gcgagtaacc tgctttcaga aggtttttac tcattggttt acccataatc cccaggccaa
     3061 taaaaccaac tttcatagtc atatcaatca tctctcttgt tgcggtggtg gttatttttt
     3121 aaaggtatca gccagtttct gagtggcaga gcggaagacg ccaagatcgc tgccgacagc
     3181 cacaaacgtc gcgccccatt ccagataacg acgcgcatcg gcttcgaccg gcgcgagaat
     3241 accgctgggt ttgccgtgcg cactggcacg gttaaaaatg tgctgaattg ctttttgtac
     3301 atccgggtgc gaggcattgc cgagatggcc taatgccgca gccagatcgc tggggccgac
     3361 gaagataccg tctacacctt cggtagcggc aatggcatcg acgttatcta cgccctgctg
     3421 actttctatc tggaccagaa tggtgatgtt cttgttcgac tgagcgaaat aatccgccac
     3481 ggtgccaaac atattggcgc ggtgagaaac ggagacgccg cgaatgcctt ccggtgggta
     3541 acgggttgat gccaccgcct gctctgcttc ctcttttgtt tctacaaaag gaatcaggaa
     3601 gttatagaaa ccgatatcca gaagacgctt aataattacc ggctcgttgg tcggcactcg
     3661 cactactggc gcgctggcgc tgcctttcag ggccattaac tgcggaataa acgtggagat
     3721 atcgtttggc gcatgttcgc catccagcac cagccagtca aacccagcca aaccaagaac
     3781 ttcagtgcta atcgggttag agagtgctga ccagcaacca atttgtacct gtttcgcagc
     3841 cagtgcggct ttgaatttat tcgggaaaac atcgttattc atcgcttata cctttgctta
     3901 tttctgcaat tccatacgtt taatgtcgcc aactacgaag aggtagcaga ccatcgccat
     3961 cagcgctgaa catcccacga aaaccagtgc tgcattgaag gagtgcagtt cacttaccag
     4021 gtagccaatc accagtggag tgacaatgga ggcaacattg ccaaagacgt taaagacgcc
     4081 gccacagagg ccaacaatct ctttcggcgc ggtgtcagaa atcaccggcc agcccagagc
     4141 accaaatcct ttgccaaaaa aagccagcgc catcagcatg accaccagcg tggtgttgtt
     4201 ggtgtagtta cataagatga tggtggaagc cagcaacatt cccagcacaa tcggtagctt
     4261 acgtgccagg gtcagggata aaccgcgttt gatcagataa tccgagaaga cacctcccag
     4321 cacgccgccc gcaaaaccac acagtgccgg aatcgaggcg accagaccca ctttcagaat
     4381 cgacatgcct ttttcctgca ccagataaat cgggaaccag gtgaggaaga accaggtgat
     4441 ggtgttgata aaatattgtc cgaaaaatac gcccaacatc atgcggttag agagcaattg
     4501 cttgatgtaa tgcagtttgg gaccgcttgc tgccgcgctg cccggctttt tgtggtccat
     4561 atcgaccacc gcgccatttt cagagataaa cttcagctct tccgcagaca tacgtgggtg
     4621 atctgtcggg ttatgaatca atttgatcca cagcgccgtc agcacaaaac caatcacccc
     4681 cataacggta aagacgtgct cccagcccca ggcgaaagtc agccagccaa gcagaggcga
     4741 aaagagcgcc agcgagaaat attgcgccga gttaaagatg gcggaggcag taccacgttc
     4801 tttcgtcggg aaccaggcgg cgacaatacg ggcgttcgcc gggaatgatg gcgcttccga
     4861 gaagccgagc ataaagcgca taaagaacat ggagatccct gcccaggcca gcgggaacat
     4921 atcaacaaag ccttgcagga aagtgaatag cgaccagaaa aagaggctat aggtataaac
     4981 ttttttcgag ccaaacttat caagcagcca gccgccgggg atttgcatca gcaagtaggc
     5041 ccagccaaaa gcagaaaaga tgtaacccat cgaaaccgca cttaactgca actcttttgc
     5101 cacttcggta ccagcaatag acagcgttgc acgatcggcg tagttaacgg cggtaacaat
     5161 aaaaataatc agtaatatta aatagcgggt atgcacgcct ttcttttttt cgtcaacggt
     5221 gtccagaatc attttattta cctcgggtac ttatgctgat ttttattatt atggggaagg
     5281 tgttatttat gagtttcatt tatgccgtaa cggcaatgaa ctcgggaatt agtataagca
     5341 gcgcgagaat aataatcatt gtgcaaatgc taatttaatt aatgctattt aaatattatt
     5401 ttgagcatat gcacataagg ttgcgcgcta aagcacagat ttgcgcttta ccttgccggg
     5461 cggcactgca atccctgaaa tgattgacat tgatcacatt tctgcgttta aactcctgac
     5521 attcttattt cacccaatga agtcatttat ttttaaatga gaccaggtcc tcattttaat
     5581 aacccctggc tggagaatat agtacaatgg ccaacatcga aatcagacaa gaaacgccaa
     5641 ctgcgtttta tataaaagtt cacgacacag ataatgtggc aattattgtt aatgataatg
     5701 gcctgaaagc aggaacgcgt tttccggatg gactggaatt aattgaacat attccccagg
     5761 ggcataaagt cgcattgctg gacattccgg ctaatggtga aattattcgt tatggtgaag
     5821 tgattggtta cgccgtgcgt gcaatcccac gcggaagctg gctcgacgaa tcaatggttg
     5881 tactaccgga agcgccgccg ttacacacgc tgccactggc aaccaaagtc ccggccccct
     5941 taccgccgct ggaaggatac acctttgagg gctatcgcaa tgccgatggc agcgtgggca
     6001 ccaaaaacct gctcggcatc accaccagcg tccactgtgt ggcaggcgtg gtggactatg
     6061 tagtaaaaat cattgaacgc gatctgctac cgaaataccc gaacgtcgat ggcgtggtgg
     6121 ggctgaatca tttgtacggc tgtggcgtgg cgattaacgc accggcggca gttgtgccta
     6181 ttcgtaccat ccacaatatt tcgctgaacc ctaactttgg cggcgaagta atggtgattg
     6241 gtctgggttg tgaaaagttg cagcctgagc gcctgctggt cggcacggat gatgtgcaag
     6301 ctattccagt agaaagcgcc agcattgtca gtttgcagga tgaaaagcat gtcggttttc
     6361 agtccatggt cgaggatatt ttgcaggtcg ccgaacgcca tctacaaaaa ctgaatcaac
     6421 gtcagcgaga aacctgtccg gcttcagaac tggttgtcgg catgcagtgc ggtggcagcg
     6481 atgccttttc tggcgtaacg gcaaacccgg cggttggcta tgcgtctgat ctactggtgc
     6541 gctgcggcgc aacggtgatg ttctcagaag tcacggaagt gcgtgacgcg atccatctgc
     6601 tgacaccacg cgcagtgaac gaagaggtcg gcaaacggct gctggaggag atggagtggt
     6661 acgataacta tctcaatatg ggaaaaaccg accgcagcgc caacccttcg ccgggcaaca
     6721 agaaaggcgg tctggcaaac gtggtagaga aggcactcgg ctccattgct aaatcgggta
     6781 aaagcgcaat tgttgaagtg ctgtcgcccg gtcaacgccc gactaaacgc ggattaattt
     6841 acgccgcgac gccagccagc gattttgtct gtggcacgca acaggtggct tcgggtatca
     6901 ccgtgcaagt gtttacgacc ggtcgtggta cgccgtacgg cctgatggcg gtacccgtca
     6961 ttaaaatggc gacccgcacc gagctggcga accgctggtt tgatttaatg gatattaacg
     7021 cgggcactat cgccaccggc gaagaaacca ttgaagaggt gggctggaag ttgttccact
     7081 ttattctcga catcgccagc gggaagaaga aaaccttctc ggatcaatgg gggctgcata
     7141 accagctggc agtgtttaac ccggcaccgg tgacctgatt tcttttggaa cgtccctcgc
     7201 aaaacatggc cttagtgcca tgtttttatt gtttaaagcc cccacgtcca ttaataatgc
     7261 atttgcatta ccttagttca agcttataat ttgagcagaa aacaggctgt aaaaggacag
     7321 tgaatcatgc ccgctaatgc tcgctctcac gctgtactga ccactgaatc aaaggtcacg
     7381 atacgcggac aaacaactat ccccgcacca gtgcgtgagg ccttaaaact gaagccaggc
     7441 caggacagca ttcattacga aattctgcct ggtgggcaag tctttatgtg ccgactggga
     7501 gatgaacagg aggatcatac tatgaatgca tttttgcgtt ttctggatgc agatatccag
     7561 aacaacccgc aaaaaactcg tccattcaac attcaacaag gaaagaaact tgtcgctggc
     7621 atggacgtca acattgatga tgagattggc gacgacgaat aatggatttt ccacaaaggg
     7681 ttaatggttg ggcgctatat gctcatccct gttttcagga aacctacgac gctttagttg
     7741 ccgaagtcga gacattaaag ggaaaagatc ctgaaaatta tcagagaaaa gccgccacaa
     7801 agttattggc ggtagtccat aaagtgattg aggagcatat cacggtcaat ccatcatcac
     7861 cggcattccg tcatggcaag tcgttaggct ctgggaaaaa taaagactgg tcacgggtaa
     7921 aatttggtgc tggtcgttat cgtctcttct ttcgttatag tgaaaaagag aaagtcatca
     7981 ttctgggatg gatgaacgat gaaaacactc tgcgcaccta cggtaaaaaa acagatgcct
     8041 ataccgtatt cagcaaaatg ttaaaaagag gacatcctcc tgccgactgg gaaaccctca
     8101 cccgagaaac agaagaaacc cattgatggt gttcacaatg cccgccgaac gtattcgtgt
     8161 tgaccagcgg gcaccctttc tcactccccg accagaatca cttcaacccc agcctttcgc
     8221 agtccttcca gactatccgc aggaatgcct tcatcaacaa tgatcatgtc gatacgttga
     8281 gtatcaatga tcttatgtaa actggaacga ttgaatttac tggaatcggt gaccacgatg
     8341 atccgttcca caacttcgca catccgacgg tttaaacggg cttcatcttc attatgtgtg
     8401 ctgacgccgc gctccagatc gatcgcatct acaccaagaa acagcatatc gaagtggtaa
     8461 ttttgcagcg attgctcagc ctgatcgccg taaaaagatt gcgactgacg gcgcaaatgc
     8521 ccgccggtca tcagcagctc aacgccttcc gcttccagca acgcattagc cacgttcata
     8581 ccgttggtca tcgcaattac gtcagtgtgc ttacgcatca gacgagcaat ctcaaaagtg
     8641 gtagtcccgg aatcgaggat cacccgatga ccaggctgaa tcaactcaac ggcagctttc
     8701 gcaacgctgc gtttcatcgc ggtgttcagc gcgcttttat cttccactga tggctcgact
     8761 gacggcgtcg tgctatcgca gatcaacgcg ccaccatagg cacgcacagc gatcccctgc
     8821 ttttccagaa acgccagatc attgcggatc gtcacagtag atacgccata caatgccgac
     8881 agatcgttaa cctgcacact cccttgctgt cgcagacgct gaatgatctg ttctcgtcgc
     8941 tcgctggtgc cagtcactcg cttctcacct gaagcgtcgg tattactcat agtaagtcct
     9001 ttcgtaaaac tttcgtttca tttcgttttg cctattaacg cctttctatt aagcaaatgc
     9061 aagcccacct tgcccattga cgcaagctac tctcgtttca gtgactttca ttatgtttct
     9121 tttgtgaatc agatcagaaa actattatct ttcgttttat ttttatctca ccatgacgca
     9181 gtatcaactg aaacaaaacg aaagattaat atcgcagtaa tctgaactgg agaggaaagt
     9241 gaaacatctg acagaaatgg tgagacagca caaagcgggc aaaacaaatg gaatttatgc
     9301 cgtttgttcc gcacatccgc tggtgctgga ggctgcaatc cgctacgcca gtgcaaacca
     9361 aacgccgtta ctgattgaag caacctccaa tcaggtagac cagttcggcg gttataccgg
     9421 aatgacgccc gccgattttc gcggctttgt ttgtcagctc gccgactcgt tgaatttccc
     9481 gcaggatgcg ttgattctgg gtggtgacca tctggggcca aaccgctggc aaaacctgcc
     9541 ggccgctcag gcaatggcca atgccgatga tttgattaaa agctacgttg cggcaggatt
     9601 caaaaaaatc caccttgatt gcagcatgtc ctgtcatgac gatccgattc ccttaactga
     9661 tgacatcgtg gctgaacgcg ccgcccgtct ggcgaaagtg gcggaagaaa cctgtcttga
     9721 acactttggc gaagccgatc tggagtatgt cattggtacc gaagtgccgg tacctggcgg
     9781 cgcgcatgaa accttaagcg agctggcggt caccacgccg gatgccgccc gcgccacgct
     9841 ggaagcccat cgtcacgcct ttgaaaagca aggtttgaat gccatctggc cacgcatcat
     9901 tgccctggtg gttcaacccg gcgtcgaatt cgatcacacc aacgttattg attatcagcc
     9961 cgccaaagcg agcgccttaa gccagatggt cgaaaactac gaaacgctga ttttcgaagc
    10021 gcactctacc gattatcaaa cgccgcaatc gctgcgccag ctggtgattg accactttgc
    10081 cattctgaaa gttggcccag cgctgacctt cgccctgcgt gaagctctgt tctctctggc
    10141 ggcgattgaa gaagaactgg tgccagcgaa agcctgttct ggtctgcgtc aggtgctgga
    10201 agacgtgatg ctcgaccgcc cggaatactg gcaaagccac taccacggtg acggcaacgc
    10261 gcgtcgtctg gcgcgtggtt atagctactc ggatcgcgtg cgctattact ggccggacag
    10321 ccagattgat gacgctttcg ctcatctggt tcgtaatctg gcggattcac caattccgct
    10381 accgctcatc agccagtatc tgccgctgca atacgtgaaa gttcgctccg gcgagctgca
    10441 accgacgcca cgggaactca ttatcaacca tattcaggac atcctggcgc agtaccacac
    10501 agcctgtgaa ggccaataag caaaacaaag aggaacacgc tatgccaaat attgttttaa
    10561 gccggattga tgaacgcttg attcacggtc aggtcggcgt tcagtgggtc ggatttgcgg
    10621 gggcaaatct ggtgctggta gccaacgatg aggttgccga agatccggta caacaaaacc
    10681 tgatggaaat ggtactggca gaagggatcg ccgtacgttt ctggacgctg caaaaagtta
    10741 tcgacaacat tcatcgcgcc gccgatcgac agaaaatcct gctggtttgt aaaacacccg
    10801 ccgatttcct gacgctggtg aaaggtggcg ttccggtgaa tcgcattaac gttggcaata
    10861 tgcactacgc caatggcaaa caacaaatcg ccaaaacggt ttctgtggat gcgggcgata
    10921 tcgcagcatt taacgacctg aaagccgctg gggtggaatg cttcgttcag ggcgtcccga
    10981 cagagcctgc tgtagacctc tttaaattac tttgagggat tcatcatgga aatcagcctg
    11041 ttgcaggcat ttgcgttggg cattatcgcc tttatcgctg gcctggatat gtttaacggc
    11101 ctaacacata tgcaccgccc ggtggtcctc ggcccgttgg tcgggctggt acttggcgat
    11161 ctgcataccg gaattttaac cggcggtacg ctggaactgg tgtggatggg gctggccccg
    11221 ctggcgggcg cacagccgcc taacgtgatt atcggtacta tcgtcggcac ggcgtttgcc
    11281 attactactg gcgtgaaacc cgatgtcgcg gtcggtgtcg ccgtaccttt cgctgtcgca
    11341 gtacagatgg ggattacctt cctgttctcg gtgatgtctg gcgtgatgtc tcgctgcgac
    11401 cggatggcgg agaacgccga tactcgcggc attgaacgcg tgaactatct ggcactgctg
    11461 gcactcggca ctttctattt tctctgcgct ttcctgccta tctacttcgg cgcggaacat
    11521 gcaaaaacca tcattgatgt cctgccgcaa cgattaatcg acggcctcgg cgtcgcgggc
    11581 ggcatcatgc cagcaatcgg ttttgccgtg ctgctgaaaa tcatgatgaa aaacgtctac
    11641 atcccctact tcatcctggg ctttgttgcc gcagcctggc tcaagttacc ggtgctggct
    11701 attgctgccg ctgcgctggc gatggcgctg atcgacctgc tgcgtaaatc tcctgaaccg
    11761 actcaacctg cggcacagaa agaggaattt gaagatggca tctaatcaaa ccaccctgcc
    11821 gaacgtcagt gaaaacgaag aaacattgct gactggcgtc aatgaaaacg tgtatgaaga
    11881 tcagagcatt ggcgcggagc tgacgaaaaa agatatcaac cgcgtcgcct ggcgttccat
    11941 gctgttacaa gcgtctttta actacgaacg tatgcaggct tctggctggt tgtacggtct
    12001 gctgcctgct ctgaaaaaga tccacactaa taaacgcgac ctggcgcgcg ccatgaaggg
    12061 gcatatgggt ttcttcaata cccatccgtt tctggtgaca tttgttatcg gcattatcct
    12121 tgcgatggag cgttctaagc aggacgttaa cagtattcag agcaccaaaa ttgccgtcgg
    12181 tgcgccgctc ggcgggattg gcgatgcaat gttctggcta acgctactgc cgatttgtgg
    12241 cgggattgga gccagtctgg cattgcaagg ctccattctt ggcgcagtcg tctttattct
    12301 gctgttcaac gtggtacacc tggggctgcg ttttggtctg gcgcattatg cttaccgcat
    12361 gggcgtggcg gcgattccac tcattaaagc gaataccaaa aaagtcggcc atgctgcgtc
    12421 tatcgttggg atgacggtaa tcggtgcgct ggtggcaacc tatgttcgtt taagcaccac
    12481 gctggaaatc acagcgggcg acgcagtggt taagttacag gctgatgtta tcgacaaact
    12541 gatgcctgcc ttcttaccgt tggtctacac cctgaccatg ttttggttgg tacgccgcgg
    12601 ctggagtccg ctgcgtctga ttgcagttac cgtggttctc ggcatcgtcg gtaaattctg
    12661 ccatttcctt taaatacaat gaggtattag atgttaagta ttattttgac agggcatggc
    12721 gggtttgcca gcggcatgga aaaagcaatg aagcaaattc tcggtgagca atcgcagttt
    12781 atcgccatcg attttccgga aacctcaagc accgcgctgc ttacatcgca gcttgaagag
    12841 gccatcgccc aactggattg tgaagacggc attgtttttc tgacggattt gctgggcggc
    12901 acgccgtttc gcgtagcttc aacgctggcg ctgcaaaaac cgggctgtga agtgatcacc
    12961 ggcaccaatt tgcaactgct actggagatg gtgctggagc gcgaagggtt aagcggtgaa
    13021 gaattccgtg tacaggcgct ggagtgtgga catcgtgggc tgaccagtct ggtggacgag
    13081 ttaggccgct gccatgaaga atgtccggtc gaggaaggga tatgacacac gttctgcgcg
    13141 ccagaaggct gctgactgaa gagggatggc tcgatgacca tcagttgcgt attgctgacg
    13201 gtgtcatcgc agcaatcgaa ccgattccag tgggcgtgac tgaacgcgat gcggaactgc
    13261 tctgccccgc ttacatcgac acccatgtac acggtggtgc gggcgttgat gtaatggatg
    13321 acgcgccgga tgtactcgac aagctggcaa tgcacaaggc acgcgaaggt gtcggcagtt
    13381 ggttgccgac taccgtaacc gcgccgctta acaccattca tgcggcgctg aaacgtattg
    13441 ctcaacgttg ccaacgcggc ggacctggtg cgcaagtgct ggggagttat ctcgaaggac
    13501 cgtacttcac gccgcagaat aaaggcgcgc atccgccgga attgtttcgc gagcttgaaa
    13561 ttgccgagct ggatcaattg attgccgttt ctcagcacac cttacgcgtg gtagcgctgg
    13621 caccggaaaa agagggggca ttgcaggcca tccgccatct taaacagcaa aatgtacgag
    13681 tgatgctggg gcatagcgcg gcgacctggc aacaaactcg cgccgcgttt gatgctggtg
    13741 ccgacggcct ggtgcattgc tataacggga tgacaggttt acatcaccgc gaaccgggaa
    13801 tggttggcgc gggattaacg gacaagcgcg cctggctgga actgatagcc gatggtcatc
    13861 atgtgcatcc ggcggcgatg tcgctgtgtt gttgctgtgc aaaagagaga atcgtgctga
    13921 tcaccgacgc gatgcaggca gccgggatgc cggatggtcg ctatacgtta tgtggcgaag
    13981 aagtgcagat gcacggtggc gttgtccgta ccgcgtccgg tgggctggcg ggcagtacgc
    14041 tgtctgttga tgcggcagtg cgcaacatgg tcgagttgac gggcgtaacg cctgcggaag
    14101 ccattcatat ggcatcgctg catccggcgc gaatgctggg tgttgatggt gttctgggat
    14161 cgcttaaacc gggcaaacgc gccagcatcg ttgcgctgga tagcgggctg catgtgcaac
    14221 aaatctggat tcagagtcaa ttagcttcgt tttgatattt tgctccttta ttgggccttc
    14281 acttcccccg taaggccttt ctttttcttt cgttttgatc tgtgcagcgg tgtcggatgc
    14341 gacgctaacg cgtcttatgc gacctacggt acgccgcatc cagcaatcgg tgaacatagc
    14401 gacgtcatta cgcaattctt taatctcccc ttgtcttttc tttcaatgat cacagatttc
    14461 gttttatttc ctttctctcc attgaacttt cggtttcttt tctatagatt ttaatcaacg
    14521 aaagacatca ccaagtgaaa tgaaacgaaa ggcaagtgaa agcgacaacg cccgacgtca
    14581 agttcatcag actaaggatt gagttatgcc agaaaattac acccctgctg ccgccgcaac
    14641 cggtacatgg actgaagaag agatccgcca tcagcctcgc gcatggatcc gttcactcac
    14701 caacatcgac gcgctacgtt ctgcgctcaa taacttcctt gaaccgttac tgcgcaaaga
    14761 gaatctgcgg atcatcctga ccggagccgg aacctcggca tttatcggtg acatcatcgc
    14821 gccgtggctc gccagccata ccggtaaaaa cttcagcgcc gtaccgacca ccgatctggt
    14881 caccaatccg atggactacc tgaacccagc tcatccgctg ctgttgatct ccttcggtcg
    14941 atccggcaac agcccggaaa gcgtcgctgc cgtggaactg gcaaatcaat ttgtaccaga
    15001 atgctatcac ctgccgatca cctgcaacga agcgggcgct ctttaccaaa acgcgatcaa
    15061 cagcgataac gcgtttgccc tgctgatgcc cgcagaaacg cacgatcgcg gctttgcgat
    15121 gaccagcagc attaccacca tgatggccag ctgcctcgcg gttttcgcac ctgagacgat
    15181 caacagccaa accttccgcg acgtggcgga tcgttgccag gcgatcctga cctcactggg
    15241 cgatttcagc gaaggtgtgt ttggttacgc accgtggaaa cggatcgttt atctcggcag
    15301 cggtggctta cagggcgcag cacgcgagtc ggcgctgaaa gtgctggaac tgactgcggg
    15361 taaactggcg gccttctatg attccccgac cggattccgt catggcccga aatcactggt
    15421 cgataacgaa acgctggtgg tggtatttgt ctcaagccac ccttacaccc gtcagtatga
    15481 tcttgatctg ctggctgaac tccgccgtga caaccaggca atgcgcgtaa tcgccatcgc
    15541 cgcggaaagc accgacatcg tcgctgccgg tccacatatt atcctgccgc cgtcacgtca
    15601 ctttatcgac gttgagcagg cattttgctt cctgatgtac gcccagacgt ttgcactgat
    15661 gcagtcgctg cacatgggca atacgccgga taccccatca gccagcggca ccgttaaccg
    15721 cgtggtgcaa ggcgtaatca ttcatccgtg gcaggcataa gaggatcgca ttatgagcat
    15781 tatctccact aaatatctgt tacaggacgc ccaggccaat ggctacgcgg tgcctgcttt
    15841 taacattcat aacgccgaga cgatccaggc gatcctcgaa gtgtgcagtg aaatgcgatc
    15901 gccggtgatc ctcgccggaa cgccggggac ctttaaacat atcgcgctgg aagagatcta
    15961 cgccctgtgc agcgcctatt ccacaaccta caacatgcca ctggcgctgc atctcgacca
    16021 ccacgaatcg ctggatgata ttcgccgtaa agtccacgca ggtgtgcgca gtgcaatgat
    16081 cgacggcagc cacttcccgt ttgccgagaa cgtgaagctg gtgaaatcgg ttgttgactt
    16141 ctgccactca caggattgca gcgtggaagc agaactgggc cgcctgggcg gtgttgaaga
    16201 tgacatgagc gttgacgccg aaagtgcatt cctgaccgat ccacaagaag ctaaacgctt
    16261 tgtcgaactg actggcgtcg acagcctggc ggtagcgatt ggtacggcgc acggcttata
    16321 cagcaaaacg ccgaagattg atttccagcg gctggcggaa attcgtgaag tggtggatgt
    16381 tcctctggtg ctacatggtg ccagcgatgt tccggatgaa tttgtccgtc gcactattga
    16441 acttggcgtc acaaaagtga acgttgccac agaattaaaa atagccttcg ctggcgcggt
    16501 taaagcctgg tttgcggaaa atccgcaggg taatgatcct cgttattata tgcgcgtcgg
    16561 aatggatgcg atgaaagaag ttgtcagaaa taaaattaat gtctgtggtt cagcgaatcg
    16621 aatttcagca taatcattag ttttttatga tttatcccaa tgtacttccc gattaatatc
    16681 ggggagtgcc ttaatggaaa aggagatgac taaaccttaa taaatacatc agtacaatat
    16741 cgcaacaata atatattaaa aaaaattata ttattcaact ttatggtgag gattacacaa
    16801 tgagcagtcc aaatattctc ttaacccgta ttgataaccg tctggttcac ggtcaggttg
    16861 gcgtgacctg gacatccacc atcggtgcga atctgctggt agtcgtggat gatgttgtcg
    16921 ctaacgatga tattcaacag aaattaatgg gtattaccgc agaaacctac ggctttggca
    16981 ttcgtttctt tactatcgaa aaaaccatta acgtcatcgg caaagctgca ccgcatcaga
    17041 agatcttcct gatttgccgt acgccacaaa cggtacgtaa attggtagaa ggtggcattg
    17101 acctgaaaga tatcaacgtc ggcaatatgc atttctcgga agggaaaaag caaatcagca
    17161 gtaaagttta tgtcgatgac caggatctcg cggacttacg ttttattaaa caacgtggcg
    17221 tgaatgtttt cattcaggac gtccctggcg atcaaaaaga acaaatccct gactaaatct
    17281 aaaatcgcct taatattggt ttgaggtaat aaaaatgcat gaaataaccc tacttcaggg
    17341 attatccctg gcggcgttag ttttcgttct ggggattgat ttttggctgg aagccttatt
    17401 tttattccgc ccgataatcg tttgtaccct aactggcgct attctcggtg atattcagac
    17461 tggcttaatt accggtggtc tgacagagtt ggctttcgcc ggattaaccc ctgcaggtgg
    17521 tgttcagccg cccaacccga ttatggcggg tctgatgacc accgtcattg catggtctac
    17581 gggcgttgat gccaaaacag caattggtct tggcctgccg tttagtttgt taatgcagta
    17641 cgtcattctg ttcttctatt ccgctttctc attatttatg accaaagccg ataaatgcgc
    17701 gaaagaggcg gatacggcag cgttttcccg gcttaactgg acaacgatgc tcatcgtcgc
    17761 ttcagcgtat gcggtgattg ctttcctctg tacttacctg gcacaagggg cgatgcaggc
    17821 gctggtgaaa gcgatgcccg cctggttgac ccacggcttt gaagtggctg gcggtattct
    17881 gcctgccgtt ggttttggct tgctgctgcg cgtaatgttc aaagcgcaat atatccctta
    17941 cctgatcgcc ggtttcctgt ttgtttgcta catccaggtc agcaacctgt tgccggttgc
    18001 cgtactgggc gcaggctttg cggtgtatga gtttttcaat gcgaaatccc ggcagcaagc
    18061 gcaaccgcag cccgttgcca gtaaaaatga agaagaggac tacagcaatg ggatctgaaa
    18121 tcagtaaaaa agatatcacc cgtctgggct ttcgttcgtc gctgctgcaa gcgagcttta
    18181 actacgaaag gatgcaggcg ggcggtttta cctgggcgat gttgccgatc ctgaaaaaga
    18241 tttataagga cgacaaaccg ggcttaagcg cggcgatgaa agataacctc gaatttatta
    18301 atacccaccc gaatctggtc ggattcctga tggggttatt aatttcgatg gaagaaaaag
    18361 gagaaaaccg cgacaccatt aaaggcctca aagtggcact gtttggccca atcgccggga
    18421 ttggcgatgc gattttctgg tttactttgt tgccgattat ggcgggaatt tgctcatcat
    18481 ttgccagcca gggaaacctg ctggggccga ttctattttt cgccgtttac ctgcttatct
    18541 ttttcctgcg cgtcggctgg acccacgtcg gttattcagt cggcgtgaag gcgatcgata
    18601 aagtgcgaga gaactcgcag atgattgctc gctcggcaac catcctcggg atcacggtaa
    18661 tcggcgggct gatcgcttcg tatgtgcata ttaacgtggt gacatcgttt gccatcgaca
    18721 gtacccacag cgtcgcgctg caacaggatt tcttcgataa agtcttcccg aacattttac
    18781 cgatggccta caccctgctg atgtattact tcctgcgggt gaaaaaagcg catccggtgc
    18841 tgttaatcgg cgtgactttt gtgctctcta ttgtttgttc cgcattcggc attttgtaaa
    18901 tggaacgagg cactgcttct ggcggtgcct ctttacttaa ggaatttcat cctgtgcaaa
    18961 cccttcagca agttgaaaac tatacggcgt taagtgaacg agccagcgaa tatttattgg
    19021 ctgtgatccg tagcaaaccg gatgccgtga tttgcctggc gaccggagcc acgccattac
    19081 tgacgtatca ttatctggta gaaaaaatcc accagcagca ggttgatatc agccagctca
    19141 ccttcgtgaa gctcgacgaa tgggtggatc tgccattaac gatgcctggc acctgcgaaa
    19201 ctttcctaca acagcatatc gtgcagctgc tggggctgcg tgaagaccag ctcatcagct
    19261 ttcgctccga agagataaat gagacagagt gcgaacgggt aacgaacctg attgcgcgca
    19321 aaggcggtct ggatttatgc gttctaggat tggggaaaaa cggtcatctt gggctgaacg
    19381 aaccgggaga aagcctacaa ccggcctgcc atatcagtca acttgatgcc agaacacagc
    19441 aacatgagat gttaaaaacc acgggtcgcc ccgtgactcg tgggatcacc ttaggcctga
    19501 aggatattct caatgcccgc gaggttttgt tattggtgac tggcgaagga aagcag
//
LOCUS       DAZGLJ010000073        19268 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000073 DAZGLJ010000000
VERSION     DAZGLJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.073"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            32..529
                     /gene="ftnA"
                     /locus_tag="ACHK49_002288"
     CDS             32..529
                     /gene="ftnA"
                     /locus_tag="ACHK49_002288"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO9262930.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(567..806)
                     /gene="yecH"
                     /locus_tag="ACHK49_002289"
     CDS             complement(567..806)
                     /gene="yecH"
                     /locus_tag="ACHK49_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO9262931.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            997..2208
                     /gene="tyrP"
                     /locus_tag="ACHK49_002290"
     CDS             997..2208
                     /gene="tyrP"
                     /locus_tag="ACHK49_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO9262932.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2270..2935)
                     /gene="yecA"
                     /locus_tag="ACHK49_002291"
     CDS             complement(2270..2935)
                     /gene="yecA"
                     /locus_tag="ACHK49_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO9262933.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3131..3217)
                     /locus_tag="ACHK49_002292"
     tRNA            complement(3131..3217)
                     /locus_tag="ACHK49_002292"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3181..3183),aa:Leu,seq:taa)
     gene            complement(3230..3303)
                     /locus_tag="ACHK49_002293"
     tRNA            complement(3230..3303)
                     /locus_tag="ACHK49_002293"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3269..3271),aa:Cys,seq:gca)
     gene            complement(3358..3433)
                     /locus_tag="ACHK49_002294"
     tRNA            complement(3358..3433)
                     /locus_tag="ACHK49_002294"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3398..3400),aa:Gly,seq:gcc)
     gene            complement(3585..4133)
                     /gene="pgsA"
                     /locus_tag="ACHK49_002295"
     CDS             complement(3585..4133)
                     /gene="pgsA"
                     /locus_tag="ACHK49_002295"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO9262934.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4190..6022)
                     /gene="uvrC"
                     /locus_tag="ACHK49_002296"
     CDS             complement(4190..6022)
                     /gene="uvrC"
                     /locus_tag="ACHK49_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO9262935.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGVDRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6019..6675)
                     /gene="uvrY"
                     /locus_tag="ACHK49_002297"
     CDS             complement(6019..6675)
                     /gene="uvrY"
                     /locus_tag="ACHK49_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO9262936.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6817..6933
                     /locus_tag="ACHK49_002298"
     CDS             6817..6933
                     /locus_tag="ACHK49_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9262937.1"
                     /translation="MVTPVTNGLPDRFTVSPATKCDKNYLYSAKMKISPLNM"
     gene            6971..7147
                     /gene="yecU"
                     /locus_tag="ACHK49_002299"
     CDS             6971..7147
                     /gene="yecU"
                     /locus_tag="ACHK49_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO9262938.1"
                     /translation="MIKIFIGHYINAFNSTVDDTLKKQPLLFLAKLMVYSAALTLFTA
                     NFHCNMTRKINEYA"
     gene            7134..7358
                     /gene="yecF"
                     /locus_tag="ACHK49_002300"
     CDS             7134..7358
                     /gene="yecF"
                     /locus_tag="ACHK49_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO9262939.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7426..8148)
                     /gene="sdiA"
                     /locus_tag="ACHK49_002301"
     CDS             complement(7426..8148)
                     /gene="sdiA"
                     /locus_tag="ACHK49_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO9262940.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFNE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSAREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILRWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(8378..9130)
                     /gene="tcyN"
                     /locus_tag="ACHK49_002302"
     CDS             complement(8378..9130)
                     /gene="tcyN"
                     /locus_tag="ACHK49_002302"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO9262941.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(9127..9795)
                     /gene="tcyL"
                     /locus_tag="ACHK49_002303"
     CDS             complement(9127..9795)
                     /gene="tcyL"
                     /locus_tag="ACHK49_002303"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO9262942.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(9810..10796)
                     /gene="dcyD"
                     /locus_tag="ACHK49_002304"
     CDS             complement(9810..10796)
                     /gene="dcyD"
                     /locus_tag="ACHK49_002304"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO9262943.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(10901..11701)
                     /gene="tcyJ"
                     /locus_tag="ACHK49_002305"
     CDS             complement(10901..11701)
                     /gene="tcyJ"
                     /locus_tag="ACHK49_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9262944.1"
                     /translation="MKLAHLGRQALMSVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(11789..12340)
                     /gene="fliZ"
                     /locus_tag="ACHK49_002306"
     CDS             complement(11789..12340)
                     /gene="fliZ"
                     /locus_tag="ACHK49_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO9262945.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDALLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(12386..13105)
                     /gene="fliA"
                     /locus_tag="ACHK49_002307"
     CDS             complement(12386..13105)
                     /gene="fliA"
                     /locus_tag="ACHK49_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO9262946.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(13270..14733)
                     /locus_tag="ACHK49_002308"
     CDS             complement(13270..14733)
                     /locus_tag="ACHK49_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFO9262947.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRVRELTVQATNG
                     TNSDSDLSSIQAEITQRLEEIDRVSEQTQFNGVKVLAENNEMKIQVGANDGETITINL
                     AKIDAKTLGLDGFNIDGAQKATGSDLISKFKATGTDNYDVGGDAYTVNVDSGAVKDTT
                     GNDIFVSAADGSLTTKSDTNIAGTGIDATALAAAAKNKAQNDKFTFNGVEFTTTTAAD
                     GNGNGVYSAEIDGKSVTFTVTDADKKASLITSETVYKNSAGLYTTTKVDNKAATLSDL
                     DLNAAKKTGSTLVVNGATYDVSADGKTITETASGNNKVMYLSKSEGGSPILVNEDAAK
                     SLQSTTNPLETIDKALAKVDNLRSDLGAVQNRFDSAITNLGNTVNNLSSARSRIEDAD
                     YATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            14988..16400
                     /gene="fliD"
                     /locus_tag="ACHK49_002309"
     CDS             14988..16400
                     /gene="fliD"
                     /locus_tag="ACHK49_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO9262948.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGNYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDSAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            16415..16825
                     /gene="fliS"
                     /locus_tag="ACHK49_002310"
     CDS             16415..16825
                     /gene="fliS"
                     /locus_tag="ACHK49_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO9262949.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            16825..17190
                     /gene="fliT"
                     /locus_tag="ACHK49_002311"
     CDS             16825..17190
                     /gene="fliT"
                     /locus_tag="ACHK49_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO9262950.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            17268..18755
                     /gene="amyA"
                     /locus_tag="ACHK49_002312"
     CDS             17268..18755
                     /gene="amyA"
                     /locus_tag="ACHK49_002312"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO9262951.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(18789..19202)
                     /gene="yedD"
                     /locus_tag="ACHK49_002313"
     CDS             complement(18789..19202)
                     /gene="yedD"
                     /locus_tag="ACHK49_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO9262952.1"
                     /translation="MKKLAIASALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
BASE COUNT         4881 a         4872 c         4564 g         4951 t
ORIGIN      
        1 gcaacaaata taacctttgt ggagcactat catgctgaaa ccagaaatga ttgaaaaact
       61 taatgagcag atgaacctgg aactgtactc ttcactgctt tatcagcaaa tgagcgcctg
      121 gtgcagctat cataccttcg aaggtgctgc cgcgttcctg cgccgtcacg cccaggaaga
      181 gatgacgcat atgcagcgtc tgtttgatta cctgactgat accggcaatt taccgcgtat
      241 taataccgtt gaatctccgt ttgctgaata ttcctcactt gatgaattat tccaggaaac
      301 ctataaacac gaacaattaa tcacgcagaa aattaacgaa ctggctcatg ctgcaatgac
      361 caatcaggac tacccaacat ttaatttcct gcaatggtat gtttctgagc agcatgaaga
      421 agagaaactg ttcaaatcga ttattgataa attaagcctg gcaggcaaaa gcggcgaagg
      481 tctgtatttt atcgacaaag aactctctac cctcgacaca caaaactaat gctctcaggc
      541 ggcaggtttt ctgccgccgt ttcaaatcag tgacggcaaa tcttactctg atcggtcgaa
      601 aaaccttctt ctgaaggtat aaatttgcct tttgctgcca gaaacgctac cagctctccc
      661 gcagtcatcc cttctgccga gcaggtgtga aaacgtgcct gttcaccaaa acgcgcttta
      721 attgctgctt ccagactggc atgcgtatat tgctcgcctg attcaatcat catatttaac
      781 acttcatgac cgtgaataga gtccatcgtc cctcctcaaa aaaagcctag cgtagcgatt
      841 gccgcttatg aagactttgc gccagcgcag gactgaatgc tttttattgt acatttatat
      901 ttacaccata tgtaacgtcg gtttgacgaa gcagccgtta tgccttaacc tgcgccgcag
      961 atatcactca taaagatcgt caggacagaa gaaagcgtga aaaacagaac cctgggaagt
     1021 gtttttatcg tggcgggaac cacaattggc gcaggcatgc tggcaatgcc gctggctgcg
     1081 gccggtgttg gttttagcgt tacgttaatc ttgttgattg ggctttgggc gttgatgtgc
     1141 tacacggcgc tattactgct ggaggtgtac cagcatgttc cggcagatac cggtcttggc
     1201 acgctggcaa aacgctatct gggacgctac ggtcaatggc tgacgggctt cagtatgatg
     1261 ttcttaatgt atgctctgac tgcggcatac atcagcggtg ccggtgaatt gttggcctcc
     1321 agcatcagcg actggacagg tatttcaatg tcggcaaccg ctggcgtgct gttgttcact
     1381 tttgttgccg gtggcgtggt ttgtgttgga acatcgctgg tcgatttatt taaccgtttt
     1441 ctgttcagcg ccaaaattat ttttctagtg gtgatgctgg tattgctgct accgcatatt
     1501 cacaaagtga atcttttaac cctgccgttg caacaggggc tggctctgtc agcaatcccg
     1561 gtgattttta cctcatttgg ttttcacggt agcgtgccga gtattgtcag ctatatggat
     1621 ggcaacgttc gtaagctacg ctgggtgttt ataaccggta gtgcgatccc cctggtggca
     1681 tatattttct ggcaggtggc aacgcttggc agcattgatt caacaacctt tatgggattg
     1741 ctggctaatc atgctggatt aaacgggctg ttacaggcgt tacgcgaaat ggtggcctct
     1801 ccgcatgttg agctggcagt gcatttattt gctgatttag ccctcgccac gtcatttctc
     1861 ggcgttgcgt taggcttatt tgattatctg gctgatttat ttcagcgttc aaataccgtt
     1921 ggtggacggt tgcaaactgg tgcaattacc tttctgccgc cgttggcgtt tgcactgttt
     1981 tatccacgag gatttgtgat ggcgctgggt tacgccggtg tggcgctggc ggtactggca
     2041 ttgattatcc cttcgctgtt gacctggcaa agcagaaagc acaatcctca ggcgggttac
     2101 cgggtgaaag gtggtcgtcc ggcgctggtg gtggtgtttc tctgtggtat tgctgtgatt
     2161 ggcgtgcaat ttttgattgc ggcagggttg ttaccagaag tggggtgatc agatagcctc
     2221 aaattcctta ttgggtgcca gaattaacgc tgacacccaa tttgacctct taatgcaggc
     2281 agcactgctt aaatttctta ccactaccgc acgggcaagg atcgttacgc cccggtttct
     2341 cttctgcttt gatcggttgc tgaacagctt tttcctgcgg atgcgccatc cagtacgcat
     2401 gtagatcaag cgccgccagt cgaatggcat ctacgctctc ttcaaacgct tctggcgaca
     2461 ttttctctac ccgctcgaag ttttcctcag taccgtgcag cgcaatcgcc tccagcgctg
     2521 gttttaacga atcgggcaac gttgaccagt cagaaagtgc cacgccccgc atatagccaa
     2581 agcaccactc ctcaacaatc gtcagctcgc tgccatcaac ttctcgcaag ccgaataacg
     2641 gctcaaactg ctccgggaat tcgttcagac gctctgcggt atcggccata tgttgaaaag
     2701 ccagattcat aaagcgcgtc atctctttct ctgacgccca gcgcggcaca tagtcagccc
     2761 caccccacac ggcaaccagc cactgttccg gttcaatctc ttgcggagaa ctcaacaccg
     2821 ccgtcaataa accgtccagc tccgccacat caaggatggc gtggtcagtg ttgtatttgg
     2881 tcagaatatc gtccagccat tccaactcac tttcgtttaa cggtcccgtt ttcatacgct
     2941 tttccttgtg gatctcaact cgccagcacc tatcttacat gccggtccgt atcagagata
     3001 ctttttgagt ggctttgctg gtgattaaaa attagggagg gtgtaacgac aagttgcagg
     3061 cacaaaaaaa ccacccgaag gtggtttcac gacactgctt attgctttga ttttattctt
     3121 atctttccca tggtacccgg agcgggactt gaacccgcac agcgcgaacg ccgagggatt
     3181 ttaaatccct tgtgtctacc gattccacca tccgggctcg ggaagaaagt ggaggcgcgt
     3241 tccggagtcg aaccggacta gacggatttg caatccgcta cataaccgct ttgttaacgc
     3301 gccaaattct tcaggccttt cagccagaca tccgcttgac gccgatgtct tttaaactgg
     3361 agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact
     3421 gagctattcc cgcattcatc aagcaatcag ttaatcactt gattttatta tcgtctggca
     3481 atcagtgccg ccgttcgatg cgttgcattc tacttacctg gcgcgatgag tcaacgatat
     3541 ttttcaccac ttttgatcgt ttgctgaaaa ttacgccgaa acgatcactg atcaagcaaa
     3601 tctgcacgcg cagcgctcaa atattgcaac attgaccaca gagtcagtac cgcagccaca
     3661 aagaaaagtg caataccggc gtactcaacc caaatgttcg gacgccacag cagccaggcc
     3721 aacgccacca tctgggcagt ggttttcact ttcccaatcc aggagacagc cacgctactg
     3781 cgtttaccca actccgccat ccattcgcgt agcgcagaaa taataatttc acgggcgatc
     3841 atcgttgccg ccggtagggt cacccaccag ctgtgataat gctcggttac cagcaccatg
     3901 gcgatagcca cgagaacttt atctgctaca gggtcaagga aagcaccaaa ccgggtactc
     3961 tggttccagc ggcgtgccag aaaaccatcg aaccagtcag tcaccgccgc gacgcagaaa
     4021 atgagcgcgg cggcaaacgg cgaccaggtg acaggcagat aaaagaccaa tacaaagaat
     4081 gggataagga tgacacggaa cagtgtaagc aacgtaggga tattaaattg cataatgacg
     4141 ggtaactatc tgttgtcagt aagattaccc ctatgttgct acagagacat caatgtttca
     4201 acgaccagaa gatcttttct gccagacctt gcgaaatacc cggcactttt gcaatttcct
     4261 cgacgctggc gttacgtaaa ccttgcaaac cgcccatata tttcaacaac atttgccgac
     4321 gttttggccc gacgccttca atggtttcca gggagctggt atttttgacc ttcgcccgtt
     4381 ttttacggtg cccgccaatc gcgtgatcgt gtgattcatc gcgaatatgc tggataacat
     4441 gcagcgcggg agaatctggc ggcaaactga atccctcacc ttccggctca aagaacagtg
     4501 tttccagccc agccttacga tctactcctt tggcaacgcc aagtagcagt ggatgatttt
     4561 tatcccatga gacatccagt tcggcgaaga catttttcgc ctgcgcaagc tggcctttgc
     4621 cgccgtcgat aaggatcaca tccgggatct tactgtcgtc aatggcttta ccataacgcc
     4681 gacgcagcac ctgattcatc gccgcataat catcgcccgg cgtgatgcct gtaatgttat
     4741 agcgccgata ctccgcacgc agcgggccgt tagcatcaaa caccacacag gaagcgacgg
     4801 tttgttcgcc catggtatgg ctgatgtcaa agcactccat ccgcttcact tccggcaatt
     4861 tcaacacgct ggcaagcgcg gtcagtcgct ggtgaacggt agattgctgc gaaagtttgc
     4921 tggttaaggc cgtcgccgca ttggtgcgcg cgagtttcag ataacgcgcc ctatcgccgc
     4981 gaggtttggt ttgaacatta atcttgcgtc ccgccagttc tgaaagggaa tcggcgagca
     5041 gcgttttatc gctaagatta aaatcgagca ggatctcacc cggtaaggtg cgcatctggc
     5101 tgccttgtaa atagaactgg cctacgaagg tttctaccac ctcgctcagt tccgtaccgc
     5161 caggcacttt cgggaaatag ctgcggctgc cgagcacttt gccctgacga atgaacaata
     5221 cgtggacaca agccatgccc gcatcgaacg ccacaccaat aacgtcgagg tcgtcgccgg
     5281 tattggaaac gaattgtttt tcggtgacgc gtcgcaccgc ctgaatttgg tcgcgaatac
     5341 gtgcagcttc ttcaaactcc agattctggc tggcagtttc catacgacta atgagttgcg
     5401 taagcacctg atcatctttg ccagacaaaa acaggcgcac atactcgacc tgctgagcgt
     5461 attcttcttc actcaccagt ccttcaacgc acggtcccag acagcgccct atctggtatt
     5521 gcagacacgg acgcgagcga ttgcgataaa cactattttc gcactggcga atggggaaaa
     5581 tcttttgcag tagcgccagt gtttcacgta cggcatagcc attcgggaac gggccgaaat
     5641 attcaccttt ggcatgcttc gcaccacgat gcatcgccag acgcgggtgg gtatcgccac
     5701 tcaggaagat aaaaggataa gatttatcat cgcgtagcaa aacgttgtaa cgcggctgat
     5761 agagtttgat gtagttgtgt tccagcaaca gcgcttcggt ttctgtatga gtaaccgtta
     5821 catcaatttg ctggatctgg gcgaccagcg cttcggtttt gcgcgaagcg aggttgctac
     5881 ggaaatagct ggaaagccgt tttttcaggt ctttcgcttt gccgacatag ataaccgtac
     5941 caccagcatc gtacatgcga taaacgcctg gctggctggt tacggttttt aaaaacgctt
     6001 ttgcgtcaaa ctgatcactc actgacttga taatgtctcc gcattacaca gaccatggcg
     6061 aattgccagg tgagtcagct caacatcgcc atgaatgttt agtttactga acatacgata
     6121 gcggtagctg ttcaccgttt tcggactgag attgagctgt tctgagatct cattgacctt
     6181 ctggcccttg gtgatcatca gcataatctg caattcacgt tcagacaaac tggcaaatgg
     6241 gctttctgtt ttttctggtt cgatctggct taacgccatt tgttgagcga tgtcagaagc
     6301 aatgtaacgc tgccctgaat agacagaacg aatcgcactc acgacttcct gcggagccgc
     6361 gcctttgctg aggtagcccg cagcaccggc ctgcatgact ttcgctggta aagggttttc
     6421 tgtatggacg gtaagcatga tgattttgac atcagctgtg gaacgcgcga ttttacgcgt
     6481 cgcctcaaga ccgccaatgc ccggcatact catgtccatt agcaccacgt caacggcatt
     6541 tgcccgacac cacttaacgg cgtcttcacc gcacgatgcc tcaccgacga cttttatacc
     6601 ctttatatct tccagaatgc gtcgtatccc tgcgcgcacc agttcgtggt catcaacaag
     6661 tagaacgttg atcaaaggaa tatctccaga aatagggata acgctactga tagttagtca
     6721 ttcgtatatt agcggttttt attgcaactt tgaaacgtta aaaatggtgg gtattcgatt
     6781 tttctctcgt ttttgtcaat tcgattgtcc acagttatgg tcacgcccgt caccaacggc
     6841 ttaccggatc gtttcacagt aagccctgcg acgaaatgtg acaaaaatta cctttattca
     6901 gcaaaaatga aaatcagccc gctgaatatg taacattaat taacccgaac aatacgggca
     6961 aaaaacattc atgataaaaa tatttattgg tcattatatt aacgcattta atagcaccgt
     7021 tgacgatacg ctcaaaaaac aaccactgct atttttagca aagcttatgg tatactccgc
     7081 cgccttaaca ttattcaccg caaattttca ttgcaacatg acgaggaaaa taaatgagta
     7141 cgcctgattt ttctactgcc gagaataatc aagaactggc aaatgaagtc tcctgcctga
     7201 aagcgatgct gacgctgatg ctgcaggcga tgggacaagc tgacgcgggc cgcgtgatgt
     7261 taaagatgga aaaacagctt gcgctgatcg aagacgaaac ccaggctgca gtattttcca
     7321 aaacggttaa gcaaattaaa caagcctacc gtcagtaata taaaaccggc tgatagcgtg
     7381 cctttcagcc ggtttttgca tctggcacgc aggacagaaa agagatcaaa ttaagccagt
     7441 agcggccgcg taacaggcaa cctgggtctt attcggtgca ttgatttttt tctgcatgtt
     7501 tttctgatgg aaattgaccg tattctcaga gattgacaaa atcatcgcta tctctgctga
     7561 tgtcttccct tccgccgtcc acctcagaat ttctttttcg cgcttgctga aattcatctc
     7621 tggcgtcatc actatttcat catttaaacg catcagagcc atcagacttt cgcgcaccag
     7681 taactgcatt tttaattgca gttcatcact aagaatgggt atttcgcgcg cgctgcaacg
     7741 ggaaaaggac aaaaagccca gcgcccggtt tggcagcatt aaatactgag tgacaccgcg
     7801 gcgtaaacca tgtgcgcgcg cggcttccca taacggctgt gcttcgttga ataagtcatc
     7861 attccacatt agatggccct gactaaagtt ttcagggttc agcaccggat caattgcgag
     7921 aaagtttttt gcctgataat aactaaccca cgcctcaggg taattggtgt agaaagccac
     7981 tttaggtcgc gtgaatggta ccgggtggcg aacacataac gaatagtaat cgtactccag
     8041 ctgctgagcc tgaagctcta tttcatggta gacctcttct gcggcctcca tcctctgaaa
     8101 acgcaacagc atcgtgcgac gccagctgaa aaaatccgta tcctgcatag taaaccgcaa
     8161 cgcccctgag agtgagtatc atttataatg ataattcaaa ctatcacata aataagattt
     8221 atatataatt tatattattc aggcaatgaa ttacttttgc aagccatcac attctcttat
     8281 gttattaatg agttatgctg atttgttaag cagttttatc acgcttgaaa tagcgtcccg
     8341 ccccgacagg tgaatcgtcg gggctgattt tttcttatta ttgcagcaga aacttctcga
     8401 ggaactggcg ggtgcgaggc tgctggggat cggcaaataa cgcttttgct gccccctgct
     8461 cgactatccg cccctggtcc ataaaaatcg cccggtccgc aacatcccgg gcaaagctca
     8521 tttcgtgcgt cacaatcacc atcgtgcgct tttcctgcgc cagctgacgg atggtgttca
     8581 ggacttcgcc caccagctct ggatccagcg ccgacgttgg ctcgtcaaac aaaatcacct
     8641 caggacgcat tgccagcgca cgtgcaatcg caacacgttg ctgttgaccg ccagacaaac
     8701 gacgtggata gctggtttct ttaccggcca gcccaacttt cgccaacagc tcgcgagcgc
     8761 gcgccgtggc ctcttctttc ggttcgcctt tgacgatcac cggcccttca ataatgtttt
     8821 ccagcaccgt acgatgcgga aacaaattaa agttctggaa gacaaacccg acgtgctgac
     8881 gcaactggcg aatcagcgat ttttgctgac ttaatgaacg tgcagtatca atagtgatat
     8941 cgccaacggt gatcgttccc gcttccggtt gttccagcag atttatgctg cgtagcaacg
     9001 tggttttgcc ggaaccactc ggaccgataa ttgccaccac ttcgccaggc tttacctcga
     9061 ggtcgatacc gtgcagcacc gtctgaccgt gaaatttttt caccaagttc ttaacttcaa
     9121 tggcactcat tttggttctc tctcctggcg attaagttga ttctcaaaat ggttctgcaa
     9181 cgtcgataat actgtcgcca tgatccagta gatgagcgaa gccgccaggt acatggtgaa
     9241 cacctccagc gtgcgcgagg taatcaactg cgcctgacgg aacagctccg gcacctggat
     9301 tgtcgcggcc agcgacgtat cctttaccag gctgataaag ctgttcgaca gcggcggcaa
     9361 cgccacccgc gccgcctgtg gcaaaatcgc gcgacgcata gtctgccatg gcgtcatacc
     9421 aatactggca gctgcttccc actgaccttt atcaatagaa gaaatggcgg cgcgcagtgt
     9481 ttcggcggca taggcggcag tgttcagcga cagaccaatc attgctgacg gaatgggatc
     9541 taattcgata ccaaactgcg gcaggccgta atagatcata aacagttgtg cgatgagcgg
     9601 tgtaccacga aaaatagaga tataaaaacg cgccagccag cgcacgggcc agattggcga
     9661 caggcgcatc agcgcgagaa taaacccgag cagtaagcca aaaaacatgc cgccgatact
     9721 gagttgcagc gtatacccag cccctttcag caggaacggc aaggaatcaa taaccagttg
     9781 tatactttct tgcatgagcg ttcccggagc taaacgtggg gatgataggc gaacagcgca
     9841 ggcgcaccgc cggtatgaat aaacagaatc ggcccttcat ctttgaagcg tttctgactg
     9901 ataccgtcaa tcagccccgc catcgctttt ccggtataca caggatcaag cagaatgcct
     9961 tccagccgcg ccagcaattt cactgcttcc atgccttcgt cgttcggcac gccgtagcca
    10021 ggtgcaaaat agtcatccca gagtataatt tccgctgatg cggtcagctc cagttctttc
    10081 gcaatcgcct gttgtaggtt aaccactttc ggcaactgat cggcaacgga acgcgacacg
    10141 gtcacgccaa tcagttcgct ttcaggcatc aggtgttcca gcccaacagc cagtccggcg
    10201 tgagttccgg cactgcccga tgcgactacc accgacgaaa tattaaccgc cccttcacac
    10261 tgttgcgcga tttccagcgc actctccaca taacctagcg cgccaagagc attagaaccg
    10321 ccaaccggaa tgacatacgg gcgaaagcct tgtgcttcga ctcgcgtcgc cagctcttcc
    10381 agttgggcat tgggatcggt cagtgcttcg cacatttcaa tctgggtatt gaacagatcc
    10441 agcaacaagc gattgccgtt ggttaaatag ttttctgcgg ttgtgccaat aggattttcc
    10501 agcagcgcca cacagtgcag accgagtttc gccgcaactg cggcagtctg ccgcacatgg
    10561 ttagactgga tcgccccggc agtaatcagc gtatcggcac cttcgcgcag agcatctgct
    10621 gcgagaaatt ccagcttacg taatttattg ccgcccattg ccatgggggt gacgtcatcc
    10681 cgtttgatga aaatttcccg tccaagataa tcagaaaagc gcggcagata ttcgagcggt
    10741 gttggcgcgc cgataaactc caaccgtgga aaacgggtta aattatgcag tggcataaca
    10801 gcctccgatg tgtgttgttg tgattttctt attatgcacg ctgaaaacgc gtaaataaaa
    10861 aaggcgctgg tgaaagcgcc cttttttgtc attatgctga ttatttggtc acatcagcac
    10921 caaaccattt ttcggaaagg gcttgcagag tgccatcttt ttgcatttcc gcaattgcat
    10981 cgttcactgc tttcagcagg tcttcatttc ctttacgcag cgccacgcca gactcctgac
    11041 gggagaatgc ttcaccggtt actgccagcg tatcgttggt tttcttcacc agatccagcg
    11101 ccgccagacg gtcaacgagg atcgcatcga tacggcctac gcgcagatcc tgatatttgg
    11161 tcgggtcatc atcataggta cgcacatcga cgccctgaac attctgccgc agccactctt
    11221 catagttggt gcccagaccg acacccactt ttttgccttt cagatcagca gccgttttaa
    11281 tggtgccttc gttacctttt ttcaccagcg cctgaatacc agaaatggtg tacggggtag
    11341 agaaatcgta ttttttcttg cgctcatcag aaatggtgac ctgattaatc accacatcaa
    11401 tgcgtttaga gtccagcgac gccagcatac cgtcccattt ggtcggtttt agtgacgcct
    11461 caacgccaag atgttttgcc agctgttggg caaattccac ttcaaaaccg gttaatttgc
    11521 cgtcatcccc ctgaaaactg aacggcggat aagtgccttc cagccctacc agcagcgtgc
    11581 cgcgctcttt aactttatta agcagacctt catctgcaaa acttttaacg ctcatgcccg
    11641 caaccagcgc tacggccatc acactcatca atgcctgacg tcccagatgt gctaatttca
    11701 tatttacccc gaatgttgtt atgtctgttt gcagtgtaga gccattgttt gtaaacacaa
    11761 aaacaactcc gctacatctt attcttattt aatatatatc agaagcaggc aggctggagg
    11821 atttctgcac aagtccggta cacgtttgac gctgatagtg ttgatacttc cgtaatgcga
    11881 tgcggtaatt gttggtgctg gtggtgggca gccacggggc gaggatttca tcaaggaaac
    11941 cgtcctgcag cggatcgagg gaaatatttt gctcgtgtag atgattcccc aaacggcgca
    12001 aacgaacgac atattcacgc accgtacctg ggctcatttc agtttgctca aaaagaaatt
    12061 gcttaaagcc gataatatcg aaaaaatcac tctgcgtttt gcaatgtaaa tcaccgcaaa
    12121 atcggcacaa tgccgcccat gatttttgtt ccgtttgcca gccattttca tcaagcagcg
    12181 cgtccaggcg ggaaatctcg attttattca ctattttgcc gtcgcgaacg agggtaatgc
    12241 gatcgagtaa tttacggcaa tgcgcgcaat gagtctggct gtgtttaaag tctttaagat
    12301 agcggcttaa tggccgtctt ttcaggtgct gcaccatcat taagaactcc tggtagtcaa
    12361 agttaaagtg cggcatttac tgacgttata acttacccag tttagtgcgt aaccgtttaa
    12421 tagcctggct gtgtaactga ctgacccgcg attccccgac ctccagcacc gcgccaatct
    12481 ctttgagatt cagctcttcc tggtaataga gggttaatac cagtttttcg cgctccggca
    12541 acgtttcgat ggcttccatc acccgctgac gcagattact gtccagcagt tgttgtagcg
    12601 ggttttctcg ctgatgatca tcagtaacca gttcgatgct atcgccgtgc tcttcgcgcc
    12661 actcatcgta ggagaagagc tggctgttat tggtgtcgag caacatttgg cgataatcgg
    12721 caatatcgat ccctaaacgt tccgctacct cagtttccgt ggcgttgcgg ccaagttcct
    12781 gctccagttg ccctattgcc tgtgccactt cacgcgcgtt gcgtcgcacg ctgcgcggca
    12841 cccagtcacg gctgcgaagt tcatccagca tagcgccacg gatacgctgc actgcgtaag
    12901 ttgtaaatgc cgttccttgt agggcgtcat agcgttcgac ggcattaagt aacccaatgc
    12961 cgcccgcctg tagcagatcg tcaagttcca cgctcgcggg cagtcgaacc tgcaggcgca
    13021 atgcttcgtg acgcaccagc gggacataac gctgccatag cgagtgttta tccattacac
    13081 cttcagcggt atagagtgaa ttcacgataa acagccctgc gttatatgag ttatcggcat
    13141 gattatccgt ttctgcaggg tttttaatcg gacgattagt gggtgaaatg aggggttatt
    13201 tgggggttac cggtaaattg cgggcagaaa aaaccccgcc ggtggcgggg gttgagcgat
    13261 aagtgtaaat taacgcagca gagacagtac gttctgcgtg gtctggttag cctgcgccag
    13321 aacagaggta cccgcttgtt gcaggatctg cgcacgagac atgttagaca cttcggtcgc
    13381 gtagtcagca tcttcgatac ggctacgggc agaagacagg ttgtttacgg tgttgccaag
    13441 gttggtgata gcagagtcga aacggttttg tactgcaccg aggtcagaac gcagattgtc
    13501 aactttagcc aatgctttgt cgatagtttc gagcgggttg gtggtagatt gcaacgattt
    13561 tgctgcatct tcgtttacca gaatcgggct accaccttct gatttgctca gatacatgac
    13621 tttattgtta ccagaagcag tctccgttat cgttttacca tctgcactaa catcgtaagt
    13681 tgcaccgtta acaactaacg tgcttcctgt tttcttagct gcattgagat caagatcgga
    13741 aagtgtggca gccttgttat caactttggt tgtcgtataa aggccagcgc tatttttgta
    13801 aactgtctca ctcgtaatca aagaagcttt tttgtcagca tctgtcacag taaatgtcac
    13861 tgacttacca tcaatttctg cagaatatac accattccca ttgccatccg ctgcagttgt
    13921 tgttgtgaat tcaactccat taaacgtgaa tttatcattc tgtgctttat tcttagccgc
    13981 tgctgcgagt gctgtagcat caatccctgt accagctatg tttgtgtcag atttagttgt
    14041 cagtgaacca tctgctgcac taacaaaaat atcattccct gtagtatctt taacagctcc
    14101 gctatctacg ttaacagtat aagcatcacc gccaacatca tagttatcag tacctgtcgc
    14161 tttaaattta gaaatcaggt cactgccagt tgctttctgc gcgccatcga tattaaaacc
    14221 gtccaggccg agagttttcg catcaatttt tgccagattg atagtgatgg tttcaccatc
    14281 attagcacca acctgaattt tcatttcatt attttcagca aggactttca cgccgttaaa
    14341 ctgagtttgc tcagatacac ggtcaatttc ttccagacgt tgagtaattt cagcctggat
    14401 agaagaaaga tcgctgtcag agttagtacc gttagttgcc tgaacagtca gttcacgtac
    14461 acgctgcagg ttgttgttaa tttcattcag cgcaccttca gtggtctgcg caacagaaat
    14521 accatcattc gcgttacggg aagcctgagt cagaccttta atatttgctg taaaacggtt
    14581 agcaatcgcc tgacctgctg cgtcatcttt agcactgtta atacgcaggc cagaagagag
    14641 acgttcaatg gcggagctca gagaagactg agatttgttc aggttattct gggtcaacag
    14701 cgacaggctg tttgtattaa tgacttgtgc catgatcttt tccttatcaa ttacaacttg
    14761 atgttattgg gctgttgccc acggtttctc accgtacccc ttgtatcggc acctgaattt
    14821 cgaactttag aaaattttag tttctctcct gttttttctt aacggctgaa atatccgtaa
    14881 tatacaactc tactctgcgc atcatcttcg tgaatcaact attaaacttt gcgcacaaat
    14941 tgccgataac ccgcttatct actgtttgca atcaaaagga attaggtatg gcttcattta
    15001 catctcttgg cgttggttcg aatctgccgc tggatacatt gctaaacaac ctgaccatcg
    15061 ctgaaaaaaa acgcttaaac ccaattactc agcaacagag tgacaatacc gcacgtctga
    15121 cagcctatgg cacattaaaa agtgcattag aaaaattcca gactgcaaat actgcgttaa
    15181 ataaagccga tctgttcaaa agcactaccg ttaccagtag caatgaagac cttaaagttt
    15241 ccaccgaagc aggtgctgcc cccggtatct ataccatcag cgtaacccaa ctggcgcagg
    15301 cgcaatcttt acgaactgat tccccgacaa tcattgcatc aacaaaagat gcactgggtg
    15361 atgagtcatc agatacacgc acaataaaaa ttacccaaga cggtcgtaaa gagccgctgg
    15421 aaatcaagct caataaagat cagacgtcat tggatgagat cagcaaagcc attaacgatg
    15481 ccgatagtgg aatttccgct agtatcgtta aagtcaaaga tggtaactat caattggtat
    15541 tgaccgccag tgaaggtctg gccaataaaa tgaccatatc tgttgagggt gacagtaaat
    15601 taaatgatct gctggcttat gacagcaaga ccaacaccgg gaatatgaag gaactggtta
    15661 acgcgcaaaa tgcacagcta aacgttaacg gtatcgatat tgaacgttca agtaacaaaa
    15721 ttaccgatgc tcctcaggga gtgactctcg atttgaccaa aaaagtcact gacgtccggg
    15781 tcacggtgac aaaaagcaac gataaagcaa ccgaagcaat taaaggctgg gtggattcat
    15841 ataactccct cattgacacc tttaacaccc tgacaaaata taaagaagta gacccaggtg
    15901 ctgaagcaca agataaaaat aatggtgcac ttctcggaga cagcgttgta cgtacaattc
    15961 aaagcggcat ccgcgcacaa tttgcaaacg gtgccagcga cagtgccttc aaaacattaa
    16021 acgagatcgg gattaagcag gacggcacga cgggtaagct gaaaattgat gatgataaac
    16081 tcaaaaaagt cctgaatgaa aatacagcat ccgtacgtga attgctggta ggcgatggta
    16141 aagaaacagg aattaccacc aaaattgcca ccgaggtaaa aggttacctt gccgatgatg
    16201 gcattattga tagcgcacag gacagtatta acgccacact gaaaaaactg accaaacagt
    16261 atctatccgt cagtgccagc attgatgaca ccgtggcgcg ttacactgca cagtttacgc
    16321 aattagatac catgatgagc aaactgaata acaccagtac ctatctcagc caacaattta
    16381 ctgcaatgag taattcctga tcacggaggt cagcatgtat accgcgagcg gctcaaaagc
    16441 ctatgcacaa attggcgtcg aaagcgccgt aatgagcgcc agccagcagc agctggtcac
    16501 catgctattt gatggagtgc tgagcgcact ggttagagca cgcctgttta tgcaggacaa
    16561 caatcagcaa ggcaaaggcg tctctttgtc aaaagcgatc aacatcattg agaacggact
    16621 gcgggtgagt cttgatgaag agagcaaaga cgaattaacc caaaacttga ttgctcttta
    16681 tagctatatg gtcaggcgct tgctgcaagc caatttacgc aacgatgtct ccgcagtcga
    16741 agaagtggaa gcattaatgc gcaatattgc cgatgcctgg aaagagtcgt tactctcccc
    16801 ttctttgatt caggacccag tctgatgaac cacgcaccgc atttgtattt cgcctggcaa
    16861 caactcgtcg aaaaaagcca gctcatgtta cgcctggcaa cagaagaaca atgggacgaa
    16921 ctcatcgcca gcgagatggc gtatgtgaat gcggtgcagg agattgcaca tctgactgaa
    16981 gagattgacc cgtccaccac gatgcaggag cagctccgcc cgatgctgcg cctgattctc
    17041 gacaacgaaa gcaaggtaaa gcagttatta cagattcgga tggatgaact ggcgaaactg
    17101 gtcggtcagt catcggtgca aaaatcggtg ttaagtgcct atggtgatca gggcggcttt
    17161 gtgctggctc cgcaggataa cctcttttga atctgaacga gtcgatggct cgcgaataat
    17221 ccgattacgg ctacgcttct aatgttcccc ttgaatggag tcgaagaatg cgtaatccca
    17281 cgctgttaca atgttttcac tggtattacc cggaaggcgg taagctctgg cctgaactgg
    17341 ccgagcgcgc cgacggtttt aatgatattg gtatcaatat ggtctggttg ccgcccgcct
    17401 ataaaggcgc atcgggcggg tattcggtcg gctacgactc ctatgattta tttgatttag
    17461 gcgagtttga tcagaaaggc agcatcccta ctaaatatgg cgataaagca caactgctgg
    17521 ccgccattga cgccctgaaa cgtaatgaca ttgcggtgct gttggacgtg gtagtcaacc
    17581 acaaaatggg cgcggatgaa aaagaagcta ttcgcgtgca gcgtgtaaat gctgatgacc
    17641 gtacgcaaat tgacgaagaa atcattgagt gtgaaggctg gacgcgttac accttccccg
    17701 cccgtgccgg gcaatactcg cagtttatct gggatttcaa atgttttagc ggtatcgacc
    17761 atatcgaaaa ccctgatgaa gatggcatat ttaaaattgt taacgactac accggcgaag
    17821 gctggaacga tcaggttgat gatgaattag gtaatttcga ttatctgatg ggcgagaata
    17881 tcgattttcg caatcatgcc gtgacggaag agattaaata ctgggcgcgc tgggtgatgg
    17941 aacaaacgca atgcgacggt tttcgtcttg atgcggtcaa acatattccg gcgtggtttt
    18001 ataaagagtg gatcgaacac gtacaggaag ttgcgccaaa gccgctgttt attgtggcag
    18061 agtactggtc gcatgaagtc gataaactgc aaacgtatat tgatcaggtg gaaggcaaaa
    18121 ccatgctgtt cgatgcgccg ctgcagatga aattccatga agcatcgcgc atggggcgcg
    18181 actacgacat gacgcagatt ttcacgggta cattagtgga agccgatcct ttccacgccg
    18241 tgacgctcgt tgccaatcac gacacccaac cattgcaagc cctcgaagca ccggtcgaac
    18301 cgtggtttaa accgctggcg tatgctctaa ttttgttgcg ggaaaatggc gttccttcgg
    18361 tgttctatcc ggacctctac ggtgcgcatt acgaagatgt cggtggtgac gggcaaacct
    18421 atccgataga tatgccaata atcgaacagc ttgatgagtt aattctcgcc cgtcagcgtt
    18481 tcgcccacgg tgtacagacg ttatttttcg accatccgaa ctgcattgcc tttagccgca
    18541 gtggcactga cgaatatccc ggctgcgtgg tggtcatgtc gaacggggat gatggcgaaa
    18601 aaaccattca tctgggagag aattacggca ataaaacctg gcgtgatttt ctggggaatc
    18661 ggcaagagag cgtagtgacc gacgaaaacg gcgaagcgac cttcttttgc aacggcggca
    18721 gcgtcagcgt gtgggttatc gaagaggtga tttaaattca tccccggcgg caagccgggg
    18781 agatttcatt acggcagtgg cgtcggtaac ggcgctttat ccagcgcggc ggcacactct
    18841 gccgtcgggc gatccacacg ctccatcgtc ataccatcgt actcaatggt gttaccgtca
    18901 cgctcaacct cgtacagctc acgttttacg gtaacgttag tgagatcatc agacatcaat
    18961 gtcaacttgc ccggcacggc aatcacccgc tgccactgac ggcaatccag ggtatcgccc
    19021 tctttagtga caatcaggct gcctatcgct tccggactga ccagcgcacg ttgcggccct
    19081 ttggtctgcc agtagcccgc tagccagtcc ggcgcggggg ttttcacgac attgttatag
    19141 ttttcgactt cggcacagcc cgccagcaac atgagcgcgc ttgcaattgc gagttttttc
    19201 atcattcgtc ctgcatgaga agaaaagagg attgtggcat taaagccctg atgtcgccag
    19261 cctttcca
//
LOCUS       DAZGLJ010000074        19132 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000074 DAZGLJ010000000
VERSION     DAZGLJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.074"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(138..1007)
                     /locus_tag="ACHK49_002314"
     CDS             complement(138..1007)
                     /locus_tag="ACHK49_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO9262953.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1397..2425)
                     /gene="epmB"
                     /locus_tag="ACHK49_002315"
     CDS             complement(1397..2425)
                     /gene="epmB"
                     /locus_tag="ACHK49_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO9262954.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            2467..3033
                     /gene="efp"
                     /locus_tag="ACHK49_002316"
     CDS             2467..3033
                     /gene="efp"
                     /locus_tag="ACHK49_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO9262955.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3085..3210
                     /gene="ecnA"
                     /locus_tag="ACHK49_002317"
     CDS             3085..3210
                     /gene="ecnA"
                     /locus_tag="ACHK49_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO9262956.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            3321..3467
                     /gene="ecnB"
                     /locus_tag="ACHK49_002318"
     CDS             3321..3467
                     /gene="ecnB"
                     /locus_tag="ACHK49_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO9262957.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            3649..3966
                     /gene="sugE"
                     /locus_tag="ACHK49_002319"
     CDS             3649..3966
                     /gene="sugE"
                     /locus_tag="ACHK49_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO9262958.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(3963..4496)
                     /gene="blc"
                     /locus_tag="ACHK49_002320"
     CDS             complement(3963..4496)
                     /gene="blc"
                     /locus_tag="ACHK49_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO9262959.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(4585..5718)
                     /locus_tag="ACHK49_002321"
     CDS             complement(4585..5718)
                     /locus_tag="ACHK49_002321"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO9262960.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(5781..6140)
                     /gene="frdD"
                     /locus_tag="ACHK49_002322"
     CDS             complement(5781..6140)
                     /gene="frdD"
                     /locus_tag="ACHK49_002322"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO9262961.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            complement(6151..6546)
                     /gene="frdC"
                     /locus_tag="ACHK49_002323"
     CDS             complement(6151..6546)
                     /gene="frdC"
                     /locus_tag="ACHK49_002323"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO9262962.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(6557..7291)
                     /gene="frdB"
                     /locus_tag="ACHK49_002324"
     CDS             complement(6557..7291)
                     /gene="frdB"
                     /locus_tag="ACHK49_002324"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO9262963.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(7284..9092)
                     /gene="frdA"
                     /locus_tag="ACHK49_002325"
     CDS             complement(7284..9092)
                     /gene="frdA"
                     /locus_tag="ACHK49_002325"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO9262964.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            9417..10394
                     /gene="epmA"
                     /locus_tag="ACHK49_002326"
     CDS             9417..10394
                     /gene="epmA"
                     /locus_tag="ACHK49_002326"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO9262965.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            10613..12115
                     /gene="yjeM"
                     /locus_tag="ACHK49_002327"
     CDS             10613..12115
                     /gene="yjeM"
                     /locus_tag="ACHK49_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO9262966.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            12167..12481
                     /gene="yjeN"
                     /locus_tag="ACHK49_002328"
     CDS             12167..12481
                     /gene="yjeN"
                     /locus_tag="ACHK49_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFO9262967.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            12478..12792
                     /locus_tag="ACHK49_002329"
     CDS             12478..12792
                     /locus_tag="ACHK49_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO9262968.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(12821..16144)
                     /gene="mscM"
                     /locus_tag="ACHK49_002330"
     CDS             complement(12821..16144)
                     /gene="mscM"
                     /locus_tag="ACHK49_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO9262969.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(16166..17134)
                     /gene="asd"
                     /locus_tag="ACHK49_002331"
     CDS             complement(16166..17134)
                     /gene="asd"
                     /locus_tag="ACHK49_002331"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO9262970.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(17231..18283)
                     /gene="rsgA"
                     /locus_tag="ACHK49_002332"
     CDS             complement(17231..18283)
                     /gene="rsgA"
                     /locus_tag="ACHK49_002332"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO9262971.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            18378..18923
                     /gene="orn"
                     /locus_tag="ACHK49_002333"
     CDS             18378..18923
                     /gene="orn"
                     /locus_tag="ACHK49_002333"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO9262972.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            complement(18936..>19132)
                     /locus_tag="ACHK49_002334"
     CDS             complement(18936..>19132)
                     /locus_tag="ACHK49_002334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9262973.1"
                     /translation="LLNFAFVIFAFLRRYGRRIIREIFPSATPQKSFFCVFISSAEII
                     SKTFNLASKSRRRKWIPALT"
BASE COUNT         4419 a         4885 c         5001 g         4827 t
ORIGIN      
        1 caggcattgc aactgattga atgcttgaga atttgtaggc cggataaggc gtttacgccg
       61 catccggcat gaccaacgat cacgttatca gtcatttcag gctggatggc tccagcctcg
      121 cattagcaaa gatgagatta tttcgcctgt ggtgcagttt tgctggtgga ttgaacacgt
      181 aagtgatgca gcgcgcgtaa gcattgatcc tgggttagcg gttgggcgtt attcgccgct
      241 acggtgcgaa catagacctg acatggcaca cccgcgagtt ttttcagtcg ttggctgaaa
      301 tcaagcattc ggcgagtgat cgcctctatt tctttacccg gcgttgcttc acgcgttttc
      361 accactgcgc cgctaaattg ttctttgcca ctttcatctt taaagcggta aagcaccgcc
      421 agataatggt cgaacaggtt atgtttccat tccggttggg aataggtgtc atattccaga
      481 ttgccatttt cctggcagtc aacgtcatat aacggcagga aatctaacag tgaggtaata
      541 cgtaatgcca gttcacgcat ttccgcatta ttaattgcat gaatgattgc tcgcgccagc
      601 atttcaatct gcaactcatt gacgactaac tcacatttgc taccgtcatg tagcgttaat
      661 acgaaggtaa gattttcgcc ttgattatcg gtaagttcca gcgtgttaac ccgacgatta
      721 atatcggcat ttttaagctc atcaaccaga atttctggga tgttttctgc cattttctta
      781 atgactttat cgcgagcttg ctcatactga aggcgtgcag cagcatccag tttatgcttt
      841 tgatgcatac gactttccag cgcaatcagc aggtcgcgca gttccatgac agacataaag
      901 aacaacgatt ctttattgcg aggttcttta attttcagcg ccagtgcaat gaaattatta
      961 ctcttgcgaa taacaccggt attaacaccc ttgatgctta tggccatgta ggttctccct
     1021 aaccatttct caattaaata attaatttta atttataagc cagataaatg ggcttggtag
     1081 taatagttgt taaaataaca taaatagccg tactcactct atataaacag aaaagaaagg
     1141 ttaattattg gtgttggcta tataaagagt aacaacagca atgtatatga ataattaaag
     1201 gttatgcaac gggcaaagat taaatttccc agtttaatga caggttgctg tacttgcgtt
     1261 tgttgctctt gccggatgcg gcgtaaacgc cttatccggc ctacagttca ttgcaaatcc
     1321 attcaatttc aatgcacaat gtaggcctga taagcgtagc gcatcaggct aatgtttaac
     1381 ttccctgttt aatcagttac tgctggcgta gctggagatc cagcggcgtt ttgctgggtt
     1441 cgccgccgat ttctcgcgcc agtttcggca ccagataacc cgacaccagt gtcagcaact
     1501 cacgcataat ctgccgtgct tcgtcatcac tcaccataaa atgcgccgcg ccctgtactt
     1561 tatcgagcac atgcaggtaa tacggcatta cgccggcatc gaacaacgca ttactcaggt
     1621 ttgccagcgt ttgtgcgtta tcgttcacac cacgtaacag aacgctctgg ttaagcaggg
     1681 tgacaccgac acggcgcaat ttagccatcg cctgacggaa cgtttcatct acctcattgg
     1741 catgattgat gtgattcacc agcaagattt gcagcgtaga acgggcaaag cgttcaacca
     1801 gcgcctcggt gatacgtgcc gggatcacaa tcggcagacg gctgtgaatc cgcagacgtt
     1861 tgatatgcgg gatggcttcc agttgtgtga gcaaccagtc cagctcgtga tctttcgcca
     1921 tcagcggatc gccgccggag aaaatcatct cgtccagttc cggatgcgca gcaacatact
     1981 caagagcagt ctgccagtta cgcttgttgc cctgattttc ggcatagggg aagtggcgac
     2041 ggaagcaata gcggcaattt accgcgcagc cgcctttgac cagcaaaagc gcccggttgt
     2101 ggtatttatg caacaaacca ggcactacgc tgtgctgttc ttccagcggg tcggtggaga
     2161 atccggacgc gacgacaaac tcatcttgcg aggtaagtac ctgacgcaaa agaggatcgt
     2221 ccggattgcc tttctccatg cgatcgataa atgagcgggg cacacgcagg gcaaacagct
     2281 ttttggcgct gcgtccggcc aacagttttt cgtccgcgtc tatattcaaa agacgcagaa
     2341 gttcatcagg atcggtcaca acatcggcaa gttgcgttaa ccaatcttct ctggatgggg
     2401 tatttagggt tacaatatgc gccattttgt ggcttagcta ccaattaaca aatttcagag
     2461 ggccttatgg caacgtacta tagcaacgat tttcgtgctg gtcttaaaat catgttagac
     2521 ggcgaacctt acgcggttga agcgagtgaa ttcgtaaaac cgggtaaagg ccaggcattt
     2581 gctcgcgtta aactgcgtcg tctgctgacc ggtactcgcg tagaaaaaac cttcaaatct
     2641 actgattccg ctgaaggcgc tgatgttgtc gatatgaacc tgacttacct gtacaacgac
     2701 ggtgagttct ggcacttcat gaacaacgaa actttcgagc agctgtctgc tgatgcaaaa
     2761 gcaattggcg acaacgctaa atggctgctg gatcaggcag agtgtatcgt aactctgtgg
     2821 aatggtcagc cgatctccgt tactccgccg aacttcgttg aactggaaat cgttgatacc
     2881 gatccgggcc tgaaaggtga taccgcaggt actggcggca aaccggctac cctgtctact
     2941 ggcgctgtgg ttaaggttcc gctgttcgta caaatcggcg aagtcatcaa agtggatacc
     3001 cgctctggtg aatacgtctc tcgcgtgaag taatgcggtt gtggtgcggc ctgcaggctg
     3061 caccatcact tattcaggtc agagatgatg aaacgcctta tcgttcttgt tttgcttgcc
     3121 agcacgctgc tcacgggctg taacaccgct cgcggtttcg gcgaagacat caaacatctc
     3181 ggcaactcca tctctcgcgc tgccagctaa tttttcttct cttccgaaaa atcatcagat
     3241 ttccgtcatt tttggcgatg ttgtctatta ttaatttgct ataggcaaac ataaataaca
     3301 ttacctaaaa ggaagacgtt atggtgaaga agacaattgc agcgatcttt tctgttctgg
     3361 tgctttcaac agtattaact gcctgcaaca ccacgcgtgg cgttggtgaa gacatttctg
     3421 atggcggtaa cgcgatttct ggcgcagcaa cgaaagcgca gcaatagcaa taagcaataa
     3481 cggtacgaca ggtatgtcgt gccgtttgtt ttttctgcga tagtcactaa ggtaatagtt
     3541 gaaattcccc tgccacctgg caaaatatcc gttcaaccat cagctttgca ggacgacctg
     3601 caaacgcctc ttttcaccgg ggacggcccc aattctccgg agcctgatat gtcctggatt
     3661 atcttagtta ttgctggtct gctggaagtg gtatgggccg ttggcctgaa atatacccac
     3721 ggctttagtc gtttgacgcc gagtgttatt accgtgacgg cgatgattgt cagtatggcg
     3781 ctacttgcct gggcgatgaa atcgttacca gtagggacgg cttatgccgt gtggacgggt
     3841 attggcgcag tcggcgcggc catcaccggc attgtgctgc tcggtgagtc cgctaacccg
     3901 atgcgcctgg cgagtctggc gttaatcgta ttggggatta ttggtctgaa actcagcact
     3961 cactaactac cgggctgctg tacccaaata aacttactga catcaaaccc ttcccgggtc
     4021 gcgactgcca gcatctcctg tttcacttcg tcagaaatgg ttggcgtgcg ggagagtatc
     4081 cacaggtagt cgcggtccgg gccgcaaacc agcgcatggc ggtattcccg atcgagtgca
     4141 ataacgttat aaccgccata gaaaggacca aagaatgaca ctttcagcgc agcgcgagtt
     4201 ggtgcgccgg taaagtacgc tttcccttca ctctgctgcc acattcctct gtcagggtta
     4261 tagcctttat taatgacatt caggccgccg tcatcacgca ggctgtatgt tgcggtgact
     4321 ttttccagtc cacgttcaaa acggtgatca aaacgggcaa tctcatacca ggtaccgaga
     4381 taacgtttgg cgtcgaaatt attcactact gttacgccac gcggcggcgt aggagaactg
     4441 caggcaacga ccagaaatgc agctgtcgct gcggcaacga gagggagcag gcgcataaat
     4501 gtttccttac tggttttttt ctaagtgtag atgacagcaa ggaaaagcgc agaaaaggtc
     4561 cgaaaattcg gacccgatga aattttactg tagagcgttg agaatctgcc aggcggcggc
     4621 gactctcgct ggattgggat agtttttgtt agccagcatc actataccca gctctttttc
     4681 tggaataaac gcgacatagc taccaaatcc gccggttgcc cctgttttat gtacccatga
     4741 tgcgcgtact gcaggagttg ggggcgtaat cgcttttacg gggtgtgctg ccagtgcaat
     4801 tttattgcca ctgccgttaa tgatgctgtc aggatttacc ggccagtcca gcatttccca
     4861 gcccaggccc tgatacatat cgccggtttg ccagtagcga gattgtgcca gttgtatccc
     4921 ttgctgaagt gttttgtcgt tgatatcacg gggattcata ttgcttcgta cccagcaggc
     4981 catatcttca atggtcgact tcacaccata agtttcagca tctaacgccc ctggcgaaac
     5041 atgaactgcc ttgccttcgc gatatcccca ggcgtaattc ttttcttctg cgggcggtac
     5101 attaatccac gtatggttga gtttgagtgg ctggaagaca cgtgtttgca tcgcctgctc
     5161 aaaactcaaa ccagacggct tcacagccag tgcgccgaac aaaccgatac tggagttggc
     5221 atacagacgt tgtgttcctg gcgcccatgc aggctgccag ttttgataga agcgcagcaa
     5281 gtcgcttgag gatttcacct catccggcac ctgcaatggc aggccgccag cagtgtaggt
     5341 tgcgagatgt aatagtgtga tcccattcca ctgtttagcg gtaagttcag gccagtattt
     5401 tgttgtggga tcgcttaact tgatttcccc tcgagcaata gcgtcgccac caagcacgcc
     5461 cgtaaatgtt ttgctgaccg aacctaactc aaacaacgtt tgctgtgtga cgggctgctt
     5521 tttggcgatg tccgcatagc cccaggtaaa gtaataaggt ttaccctgat aaattaccgc
     5581 caccgccata ccggggatct tttgttgctc tataagcggg gtaattgtgc gatgcacaat
     5641 atcgttgatt tgttgagggg cagcaaatgt ggagcaagag gcggtaatta ataaggcgca
     5701 gagcgtcgtt ttgaacataa ggtctggttt ccatacaaaa cggcccgcca taggcgggcc
     5761 ggatttacat tggcgatacg ttagattgta acgataccaa tcagcgtgac aactgtcagg
     5821 atagcagcca gaccgtagaa aacccatttg cccgcaggca cgtgaatttt cagatcgtgc
     5881 atcgcgtggt gcatacggtg taaaccacac cacagcggca gaacgatcat caggaacagg
     5941 aatacgcgac caatgaagct ctgcgcgaac gccagaacgc gctcgtagct cagcgcatca
     6001 cccggaaaca gccccagtgg cagcagaata cccaccagca ggatcatcac cggcgcaata
     6061 attgcgctcc acataccacc ggccccgaag aggccccaga ataccggttc gtcagaacgc
     6121 tttggatttg gattaatcat ctcaggctcc ttaccagtac agggcaacaa acaggattac
     6181 gatggtggca accacagtta ccgcccagag acttttgata attggctctg gtcccatttt
     6241 ttcgtctttt acaatgatat tggccgcttt cggtgccagt tcaaaccagg ttttggtgtg
     6301 cagcagagct gccgccagag tgatcaggtt aatgatcacg ataaccgggt tttgtaaaaa
     6361 gtcgacgaat cccgcccagg cttccgggcc atttttcagg gcaaacagcc cgaaaatcag
     6421 ttcaatgctg aaccacacag ccggaaccgc cgtgccttcg cgcagcatgt aaaagcgata
     6481 aaacggcaat tttttccacc aggtggacgt cattggccgt acatacggtt tacgtttagt
     6541 cgtcatgttg cactccttag cgtggtttca gggtcgcgat aagaaagtct ttcgaacttt
     6601 ctactttgcc ctgctgaatg gccgcagccg gatcgacgtg tttcgggcag acttcggagc
     6661 aatagcccac gaaagtacag ctccatacgc cgttctggct gttcaactgc gccatacgct
     6721 ccttcttacc gtggtcgcgg ctatcttcgt tataacgatg cgccagcgta atggcagccg
     6781 gaccgatgaa ctctgggttc aggccaaact gcgggcacgc ggcgtagcac agaccacagt
     6841 tgatgcaacc ggagaactgg tgatactttg ccatctgcgc cggggtctgg atgttagtac
     6901 cctgatccgc ggtgcgggag ttgccgatga tgtacggttt gatcgcttcc agactttcga
     6961 tgaagtgggt catatcgacc accagatcgc gttcaatcgg gaagttagct aacgcttcaa
     7021 ccttcatacc gtcggtgtaa tcacgcagga aggttttaca tgccagtttt ggcacgttgt
     7081 taaccatcat gccgcaggaa ccacaaatcg ccatacggca ggaccagcgg taactcaggt
     7141 ccggtgccag gttgtctttg atgtagccca gcgcatccag taatgaggta gttgcgtcat
     7201 aaggcacttc atagaatgcg ctatgcggtg cggtatcgac ttccgggtta tagcgcacca
     7261 cctcaatttt caggtttttc atctcagcca ttcgccttct ccttcttatt ggctgcttcc
     7321 gccttatcgg ctgcatccgc ttcgccaccg taaacgcgtt tagctggcgg cagcgtagta
     7381 atcttcacgt cgctgtactc caggcgagtc gtgccatcag catcgcggaa ggcgagggtg
     7441 tgtttgagga agttgacgtc gtcacgctcg gtgcaacctt cgtccagacg ctggtgtgcg
     7501 ccgcgggact ctttacgtgc cattgcggag tgcgccatac attcagcaac gttcagaccg
     7561 tggcccagtt caatggtgta gagcaggtcg gtgttgaaca cgctggaggt gtcggtgatg
     7621 cgcacgcgct tgaagcgttc ctgcagctct gccagcttgt cgatggtttt ctgcatcagt
     7681 tccggcgtac ggtagatacc gcaaccttct tccattgcca ggcccatttc gtcgcggatc
     7741 ttcgcccagt tttcgccgcc atcctggtta accagatctt tcagacgttg ttcaacgcca
     7801 gctgcctgcg cttcaattgc cgcttcgttg ccattaccgg cagttgctgc acgctctgtc
     7861 gcttgttcac cggccagacg gccgaagacc accagttccg ccagggagtt agaacccaga
     7921 cggtttgcac cgtgcagacc aacagaggaa cattcaccca cggcgaacag acctttaatg
     7981 cgggtttcac agttctgatc ggtttcgata ccgcccatgg tgtagtgtgc ggtcggacgt
     8041 accggaatcg gttctttaac cggatcgacg ccaacgtacg ctttcgccag ttcgcagatg
     8101 aacggcagac gttcatgcag ttttttctcg ccgaggtgac gcaggtcgag ataaaccaca
     8161 tcgccacgcg gcgtggagat ggtgttgcct ttacgccatt cgtgccagaa ggcctgagaa
     8221 actttgtcgc gtggacccag ttccatatat ttgtttttcg gctcgcccag cggagtttcc
     8281 gggcccatgc cgtaatcttg cagataacgg tagccatttt tgttgaccag aataccgcct
     8341 tcaccgcggc agccttcggt catcaggata ccggaacctg gcagaccggt tgggtgatac
     8401 tgaacgaatt ccatgtcacg cagcggaacg ccgtggctta gcgccatacc cataccgtca
     8461 ccggtaacga tgccgccgtt ggtgttgtaa cgataaacgc gacccgcacc gccagtagcc
     8521 ataacgaccg cgttagcacg gatctgcacc agcgtgcctt ccatcatgtt cattgctacc
     8581 acgccgcgaa catgaccatc atcaaccaga atatccagca cgaaatgttc gtcaaaacgc
     8641 tggatctgcg ggaattgcag agaggtctgg aacagcgtgt gcagcatatg gaagccggtc
     8701 ttatcggcgg cgaaccaggt gcgctcgatt ttcatgccgc cgaagcgacg tacgttgacg
     8761 ctaccatccg ggcgacggct ccatgggcac ccccacagtt ccagttgggt catttcggtt
     8821 gggcagtggt ggacgaaata atccacgaca tcctgctcac acaaccagtc gccacccgct
     8881 actgtatcgt gaaagtgata ttcgaagctg tcatgatcct gcgcgacagc ggcggagccc
     8941 ccttctgcag caacggtatg gctacgcatc gggtatactt ttgagattag tgcgattttt
     9001 gcatttggat ttgcctgcgc ggcagcaatt gcagcacgta atcccgcgcc accggcgcct
     9061 acaatggcaa gatcggcttg aaaggtttgc acgacattcc tccagattgt ttttatccca
     9121 cagccccgta cttcagggta agtgcctgaa agttacggtc tgcgaacgct attccactgc
     9181 tcctttatag gtacaacagt atagtctgat ggataagtct gaaatttgac gagatcgatt
     9241 tttttagtgc gcaagagggc taaattatca ctgaagatga ttaatttaat tactaaacca
     9301 tcagatcgtg ctttttttag tcactgccca ccgctgtttg attcctgcgt aaaatttgtc
     9361 tcgccgtcgc gttgcgagta gacttcgtgc ccttgtcaaa aactggagat ttaactatga
     9421 gcgaaacggc atcctggcag ccgagcgcat ccattcctaa cttattaaaa cgcgcggcga
     9481 ttatggcgga gatccgtcgt ttctttgccg atcgtggagt gctggaggtg gagacgcctt
     9541 gtatgagcca ggcgacggta accgatattc atttggtccc gtttgagaca cgtttcgttg
     9601 gccccgggca ttcgcagggg atgaatctct ggttaatgac cagcccggaa taccatatga
     9661 aacgcctgct ggttgccggt tgtgggccgg tattccagct gtgccgcagc ttccgtaatg
     9721 aagagatggg gcgttatcac aaccctgagt tcactatgct ggagtggtat cgaccgcact
     9781 atgatatgta ccggttgatg aacgaggtgg acgatctctt acaacaggtg ctggactgcc
     9841 cggcagcaga aagcctttct tatcaacaag ctttcttgcg ttatctggaa attgacccgc
     9901 tctctgccga caaaacgcaa ctgcgggaag tcgcagcgaa actggatttg agcaatgttg
     9961 ctgataccga agaagaccgc gacacgctgc tacaattgct gtttaccttt ggcgtagagc
    10021 caaatattgg caaagaaaaa ccgacctttg tgtaccactt tccagccagc caggcatcac
    10081 tggcgcaaat cagtaccgaa gatcatcggg tcgctgaacg ctttgaggtt tattataaag
    10141 gtattgagct ggcgaatggt ttccatgaat tgacggatgc ccgtgagcag caacaacgct
    10201 ttgaacaaga taaccgtaag cgcgcggcgc gcggtttgcc gcagcacccc attgaccaga
    10261 atctgattga agccttgaaa gtcggtatgc ctgactgttc cggcgtggca ttaggtgttg
    10321 atcgtctggt gatgttggcg ctgggcgcgg agacactggc tgaagtcatc gcctttagcg
    10381 ttgaccgggc ataattctga aaaattactg aattaacagc gaagaatggc gtgataagcg
    10441 ccattctgta gcatatgttt tcatttttcc gttaaaggcc acaatttaag actggatatt
    10501 tgatatcatc caggtatcaa ttctgtattg gtttttacgc tgcctgctca atgttgcgca
    10561 gtgcagttca gtgggcacgt gttatacacg cgctgaaatg aaggatggtt tcatgcctca
    10621 cacgataaaa aagatgagtc tgataggact catattgatg atctttactt ccgtatttgg
    10681 atttgccaat agcccatcgg cttattactt aatgggttat agtgcgattc ccttttatat
    10741 attttctgca ttgttattct ttattccatt cgccttaatg atggctgaaa tgggagctgc
    10801 ttatcgcaaa gaagaaggcg gtatctattc ctggatgaat aatagtgtcg gaccacgttt
    10861 tgccttcatt ggtacgttta tgtggttttc ctcttatatc atctggatgg tgagtacctc
    10921 cgcgaaagtt tgggtaccgt tctcaacatt cctctatggt agcgacatga cccagcactg
    10981 gcgtattgct ggactggagc ctacgcaggt ggttggtctg ctggcagtgg catggatgat
    11041 tctggtcacc gtcgttgctt caaaggggat taataaaatt gcccgcatta ctgcggtggg
    11101 cggtattgca gtaatgtgtc tgaatttagt attgctgtta gtaagcatta ctattttgtt
    11161 attaaatggt gggcatttcg cgcaggatat taatttcctt gcatcaccga acccaggtta
    11221 tcagtccggt ctggcaatgc tatcgtttgt ggtatttgct atttttgcct atggcggaat
    11281 tgaagcggtt ggtggtctgg tcgataaaac ggaaaatcca gaaaagaact ttgccaaagg
    11341 tattgttttt gccgctattg ttatttcaat cggttattcg ctggcaatat ttttatgggg
    11401 cgtcagcaca aactggcagc aggtattaag taatggttcc gttaaccttg gcaatattac
    11461 ctatgtgctg atgaagagcc tcggggtgac gctgggtaac gcactgcatt tgtcacctga
    11521 agcgtcattg tcgctgggcg tatggtttgc gcgtattacc ggactttcga tgttcctcgc
    11581 ttataccggt gcgttcttta cgctttgcta ttcaccgctg aaagccatca tccaggggac
    11641 gccgaaagcg ttgtggccgg aaccgatgac gcgcctgaat gcgatgggga tgccgtctat
    11701 cgccatgtgg atgcagtgcg ggttggttac tgtcttcatc ctgctggttt cgtttggtgg
    11761 cggtaccgca tcggcgttct ttaacaagct gacgctgatg gcgaacgtgt ctatgacgct
    11821 tccttacctg ttcctcgcgc tggctttccc attttttaaa gcacgtcagg atctcgacag
    11881 accatttgtg attttcaaaa cgcatatgtc ggcaatgatt gcaacagtgg ttgtcgtact
    11941 ggtggtgaca tttgcgaacg tcttcaccat cattcaacct gtggttgaag ccggagactg
    12001 ggacagcaca ttgtggatga ttggcggccc tgtcttcttc tcgctgttag cgatggcgat
    12061 ttaccagaac tattgcagca gagtggcaaa aaatccgcag tgggcggtgg aataacccga
    12121 tgccctatcg tctacctgac gatagggcgt aaagacagga aatgttatgg atgacaccag
    12181 ccgggacccg gcaataaccg aagatgaaat cagggcgctg caattttctg ccggggatgt
    12241 agcggaaata gagcagacga ttctctcttt tgtcgatgcc tgccacaccc gtaaagtggc
    12301 gatggtggtg ggtagcacca ttaacaccct caaagatcgc gatggtaaac ggtggggaaa
    12361 cctgccggat atttattgcg cttatcttat ccgatgtctg gtttttcgcg gggaattagt
    12421 gggatacggc gatttgtttc gtatgcgcta tagcgaaatt aagcgaccgg ttaccttatg
    12481 agtgcgcgca tgtttgtctt atgctgcatc tggtttattg tggcgttcct ctggataacc
    12541 atcacttccg cgctggacaa agagtggatg attgatggac gcgggattaa caacgtctgc
    12601 gatgtgttga tgtatctcga agaagacgat acccgtgatg ttggtgtgat aatgacgtta
    12661 ccgctctttt tcccttttct ctggttcgct ttgtggcgaa aaaaacgcgg ctggtttatg
    12721 tacgccaccg cgctggctat tttcggctac tggctgtggc agttttttct gcgctatcag
    12781 ttttgtttgt gagccggatt ggttcatccg gcacacaaac ttacaaactt cccgcctgac
    12841 gacctttgcc cgcagacgtc agcgttctcc ccgtttgttt accgttgagg ctttccagac
    12901 gcatctggaa gggcgggaat ggcatatcga taccgtgggc gtggaagcct gccagaatca
    12961 gctggtggat ctcatggcgt agcggcatac ggtgacccat ctcagcggcg taaatacgca
    13021 gctcgaaaat ctgaatcccc tgttgcagat ccaccaggaa gacttccggt gccgggttgt
    13081 cgatcaccag cgagcagcga cgcgctgcgg tgagcaggat ttccgtcact tcttcgctat
    13141 tggcatcggc aggggccggt atcgtcaaca ccacgcgcgt gaccgagtca gagagcgacc
    13201 agttgataaa ctgctcggta ataaacgcct tgttcggcac gattatctct ttacggtccc
    13261 agtcgctgat ggtggtggcg cgggtgttaa ttttcgtcac gctaccggta agatcgcgga
    13321 tcgttaccgt atcgccaatg cgaatcggtt tttcgaacag gataatcagg ccagagataa
    13381 agttggcgaa aatctcctgc aaaccaaaac cgagaccaac accgagcgcg gcaaccagcc
    13441 actgcaattt cgaccactca ataccaatca tcgagaagcc gaccagcccg ccaatcagca
    13501 tcagcagata tttggtgatg gtggtgatgg cgtagcccgt acccggcgtt aaatccaggt
    13561 gctgcaaaat cgccagttcc agcagcgcgg gcaggttgcg caccagctgc gtggtgatga
    13621 taaacaccag aatggcaatc agcaccgcac cgagggtaat tggctccaga ctttctacgc
    13681 cctgtaccgt ggaggtgaca tcccacagcg aaatattttc gaggaagccg aaagcggaat
    13741 ggatttctga ccacagcaca atgacagaaa gcagggcgat caacatcaaa attgagcgca
    13801 ccagccgcaa ggattgcgca ctgatggcat cgagatcgac ttcgctttca tcgacttcaa
    13861 ttgccccttc cgggctactg tgatgatgcg cttcctcttc gccacgcgcg cgttgcgcta
    13921 acatctctgc ccggcgatgc ttcgcccggt caaacgccag cctgcgacgc tggatcagca
    13981 tccagcggcg gataacgtga taaaccacca gtagcaggaa ccagatggca accgaggttt
    14041 caagcctcgc taacagtgcc tgcgccgttg ccagataacc caccgctgac gccagaatgg
    14101 caaccagtgg tgcgccaatc atcatgttcc acagcatatg gttggtaatg ttgtcgccgc
    14161 tgccctcttt gttgaggtac agcgggatcc cggccttttt caggctgagg gtgaccaccg
    14221 ccagcgcacc gcaaatgagg ataaagcaaa gccgtcccag cgaaccggag aattcacggt
    14281 cgtcgaggtt atcgaacatc atcagcgcca taatcagcgg cacaataagc ccgatgctca
    14341 tcaggtagta gcgcatccca cgggaaacac gttcgcgcgg ccagccaaaa tgagcgataa
    14401 acaagccgtt cgggcgggca aaggtggcgc aaatcatcac tacccacagc agcggcacgg
    14461 tggccgttac gccatcgcca atcgcgaccg ccagcggata aggccacgcc tcgcgcaagc
    14521 cgtaacccag cgtcatccac agcaccggca gcggtgacgc gacgagaatc gaccagaaaa
    14581 gagtgcgcaa cgtcagccag aagtgatcct gagtcacttt gccgactttc gccgccgaac
    14641 gttcaagaaa acgggtgaaa tagcggcgcg agtaaatact gcaaccgacc agaatcaacg
    14701 cgccaaacag cggcaaaatc gtctctttgc tggtcagcat catcacactg gctttgccca
    14761 actgactgaa ggtgtccagc gaaatgagac gacgcagatc ctgggcgatt tccagtggcc
    14821 aggcgatggt catcgggcgc acgtcagagg tccagaacag atagcgatgc gtcgcttcat
    14881 tcacctcttt cagcgcatcc tcaagttgcc cgttggagac tttcagcttg gtcagttcca
    14941 gcagtagcgt gtcgccaccc tgcaacaatg agttcagcaa ctcacgctga gtgcgtagct
    15001 gtgcttccag aatacggttt tgctcggcgg tcagcggctg accgtcggcc tgatgaattt
    15061 gccgtagcag cggctgttta ttaagcagat cctcataacg taaccgttgc acacgcaact
    15121 gcgccatttc ggtatcaagc tgttgtggtt tcggcatttc cggcagccgt gccacctgcg
    15181 cccgcaacgc ttcgccgagc aggttggacg atcccagcca ttgcgactgt tcacgcagcg
    15241 tattcagcgc ctgccgaacc tgtaacgtct ggctggtggc ctggcgctgt tgcgaggcaa
    15301 cgagatccat ccgctgcgcc tgttgattca gtgccgccga tagttcgcgg ttaattttga
    15361 attgcgcgac gatgtctttc ggcaaatcgg cgctgttttc tgccagcaat tcggtacttt
    15421 ccagcgcccg ctccgcctca agctgacgtt ggctgttcaa ctgattacgt aaggcctgca
    15481 aatacgcatc cagttgctgg ctctcttttt ccgccagctc tgagcgtaag cgcgctaatt
    15541 cctgacggtt attggcagac agctgcgcca gttccagttc atcaacgagc gctttcaggc
    15601 gtgcggagtc agactgcaac gcgaaatttt gtgcctgatt gagcggggta ttgccagtaa
    15661 gcgttcccag gcggcgctcg atctcattta actgacggcg ggcgtcggtt tgctgttgcg
    15721 gcagttgatt cagcgaatcg gcaatctcgc gggcgcgctc ctgctcttgc tgggcctgac
    15781 ggcttttatc cagcaactgg ctgctgacct ggagaatttc ctgattcagc gcgtcggtag
    15841 acattcccgg cgacacgctg cgcggctcgt cacgcatgtt gtttaattgt gcgcgcagag
    15901 tagcggagag tttcggataa ttatcgataa cttgctgata ttgtttgatg cgctcaaggg
    15961 aaccttttcg ttcctcaagc gcatttaagg cagactggag cgcctctacg acttccggct
    16021 gtgcgggttt cgccgctttt gcctgctcca gttcctgagt gatttgtttg ctatcggggg
    16081 ccgtcgcggc gtacgccccc caactgaggc accaggccat cagaaaagtg ataatcaggc
    16141 gcacgtcagc gtttcctttg atggattaga cctggtcttt tttatcgtca accaatgggc
    16201 tggcgtcgtg ttctgcttcg atctcttcag caggaagtgg ggcgggttca gcgtctggcg
    16261 taacaaaggt ttcggtagat actgccagcg gctggccaat tttcgtgaca gacaggcttt
    16321 ccagttgctc aaccagattc actttacccg gtgcaaacag gttgataacg gtagagccga
    16381 gtttaaagcg gcccatttcc tggcctttca gcagtgccac agaaccgtcg ttttccccgg
    16441 caggccaggt ccagcgcttg atgatacctt cgcgcggcgg cgtaatggtg cccgcccaga
    16501 ccgtctcaat gctgcctaca atcgtcgccc cgaccagaat ctgcgccatc gggccaaatt
    16561 cggtatcgaa caggcaaatc acgcgttcgt tacgggcaaa cagattcggc acgttctgag
    16621 cggtgagatg gttaacggag aagagatcgc ccggcacgta gatcatctca cgcagaatac
    16681 cgttgcacgg catgtgtacg cgatggtagt cacgcgggga gaggtaagtg gtcacaaacg
    16741 taccgttgcg gaacaggtcc gccatcagat agttgcctgc cagcagggct tcgaggctgt
    16801 agttgtggcc tttggcttgc aggattttat cttcttcgat tttacccaac tggctgataa
    16861 cgccatcggc aggcatgacc aggacattcg gatcggtatc gattgggcgt acttcgtcac
    16921 gcagcggacg gacaaagaat tcgttaaagg tgcggtagct ggcggtgtcc ggcttttgcg
    16981 cctctttcat gtcgaccttg tagtatttaa cgaacagatc gataaccagt tttgtcagcc
    17041 atcctgcccg cttgcttgcg ccccaacccg ccaggcgagt aagccatagt ttcggcagaa
    17101 tgtactgtag cgaaagttta aatgaattta acaaggtagc ctccaggcca ttgttttgtc
    17161 gttcctgatc cggccaacat gccggatcct gaaaaaaagg ggacgattct aacgacggtt
    17221 agcttaattg tcagtcatcc gtatcagaaa agtttttacg cgtttttacc tgcgccatgc
    17281 tttccagaat acggtgatag ttttcgaaac gggtttccgc gattttccct tcctcaaccg
    17341 cttcccggat agcgcagccc ggatcggtat cgtgtttgca atcgcgatat ttacacagac
    17401 ctaaatagtc atggaattcg acaaagccct gagtgatttg ttccggctcc aggtgccaga
    17461 ggccgaactc acgcacacca ggggaatcaa tcacatcacc gccgtgcggg aagtgataca
    17521 gccgagcggc ggtcgtggtg tgctgaccga gccccgagtt gtcagagaca tcgttggtca
    17581 ggatctcttt ttgcagcccc agcaacgcat tcagcaggct ggatttgccg acgccagact
    17641 gcccggcaaa aatgctgatg cgcccggtca acgcctcttc cagcggtttt agcccatcct
    17701 gagtatggct ggaaaccatc aatacgcgat aaccgatatt gcggtagata tccatctgct
    17761 cgttgacgaa cgccatgcct tcgtcgtcca gcaggtctat cttgttgagc acaataatcg
    17821 gctcaatctg caaggtttcg caggccacca ggtaacggtc gataatattg agcgacagct
    17881 ccggcaaaat ggcggagaca atgacaatct ggtcgatgtt ggcggcaata ggttttacgc
    17941 cgtcgtagaa atccggacgc gtcaacaccg aggtacgctc atgcaccgct tccacgatcc
    18001 ctttgacatt tacgccttcc gccgccggtt tacccggacg ccagactacg cggtcgccgg
    18061 ttaccagcga acggatggta cggcgaatat tgcagcggtg aacgtcgcca tcggcggatt
    18121 ccacatcagc gtgcatacca aagcggctga tgacgatacc ttcatcaggc tcgccaaaca
    18181 gattgtcgtc gtagtcgggc ttctccttag acgttttaag acgacgctgg tgattggcgt
    18241 tcacgcggcg ctgctggcct ttggagagtt tatttttact caatcgtaca gactcctggt
    18301 cgcccctgat gggcaaaaca tctatgatac acgcaattgt ggatcaatat agtcactgtg
    18361 aatgggtgga aaatagcatg agtgccaatg aaaacaacct gatttggatc gatcttgaga
    18421 tgaccggtct ggatcccgag cgcgatcgca ttattgagat tgccacgctg gtgaccgatg
    18481 ccaacctgaa tattctggca gaagggccga ccattgcagt acaccagtct gatgaacagc
    18541 tggcgctgat ggatgactgg aacgtgcgca cccataccgc cagcgggctg gtagagcgcg
    18601 tgaaagcgag cacgatgggc gatcgggaag ctgaactggc aacgctcgaa tttttaaaac
    18661 agtgggtgcc tgcgggaaaa tcgccgattt gcggtaacag catcggtcag gaccgtcgtt
    18721 tcctgtttaa atacatgccg gagctggaag cctacttcca ctaccgttat ctcgatgtca
    18781 gcaccctgaa agagctggcg cgccgctgga agccggaaat tctggatggt tttaccaagc
    18841 aggggacgca tcaggcgatg gatgatatcc gtgaatcggt ggcggagctg gcttactacc
    18901 gcgagcattt tatcaagctg taaaattttt cctggtcacg taagcgccgg aatccacttg
    18961 cggcggcgtg attttgacgc taaattgaac gtcttgctga taatttcagc acttgagata
    19021 aaaacgcaaa aaaaactttt ttggggggtt gcagagggaa agatttctcg tataatgcgc
    19081 ctcccgtaac gacgcagaaa tgcgaaaatt acgaaagcaa aattaagtag ta
//
LOCUS       DAZGLJ010000075        18853 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000075 DAZGLJ010000000
VERSION     DAZGLJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.075"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..850
                     /locus_tag="ACHK49_002335"
     CDS             <1..850
                     /locus_tag="ACHK49_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO9262974.1"
                     /translation="TFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKELTHKLESIF
                     NNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSDRTVRIQVG
                     YGLEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQYPTNPSESE
                     SASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVYISAIYLLDLFLFSDKIF
                     SIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSGGGGSSGGG
                     GASGRW"
     gene            complement(910..2208)
                     /gene="eno"
                     /locus_tag="ACHK49_002336"
     CDS             complement(910..2208)
                     /gene="eno"
                     /locus_tag="ACHK49_002336"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO9262975.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2296..3933)
                     /gene="pyrG"
                     /locus_tag="ACHK49_002337"
     CDS             complement(2296..3933)
                     /gene="pyrG"
                     /locus_tag="ACHK49_002337"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO9262976.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4161..4952)
                     /gene="mazG"
                     /locus_tag="ACHK49_002338"
     CDS             complement(4161..4952)
                     /gene="mazG"
                     /locus_tag="ACHK49_002338"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO9262977.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5023..5358)
                     /gene="mazF"
                     /locus_tag="ACHK49_002339"
     CDS             complement(5023..5358)
                     /gene="mazF"
                     /locus_tag="ACHK49_002339"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO9262978.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5358..5606)
                     /gene="mazE"
                     /locus_tag="ACHK49_002340"
     CDS             complement(5358..5606)
                     /gene="mazE"
                     /locus_tag="ACHK49_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO9262979.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5684..7918)
                     /gene="relA"
                     /locus_tag="ACHK49_002341"
     CDS             complement(5684..7918)
                     /gene="relA"
                     /locus_tag="ACHK49_002341"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO9262980.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7966..9267)
                     /gene="rlmD"
                     /locus_tag="ACHK49_002342"
     CDS             complement(7966..9267)
                     /gene="rlmD"
                     /locus_tag="ACHK49_002342"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO9262981.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9324..12080
                     /gene="barA"
                     /locus_tag="ACHK49_002343"
     CDS             9324..12080
                     /gene="barA"
                     /locus_tag="ACHK49_002343"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO9262982.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12312..13652)
                     /gene="gudD"
                     /locus_tag="ACHK49_002344"
     CDS             complement(12312..13652)
                     /gene="gudD"
                     /locus_tag="ACHK49_002344"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO9262983.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGKYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13673..15013)
                     /locus_tag="ACHK49_002345"
     CDS             complement(13673..15013)
                     /locus_tag="ACHK49_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO9262984.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15015..16367)
                     /gene="gudP"
                     /locus_tag="ACHK49_002346"
     CDS             complement(15015..16367)
                     /gene="gudP"
                     /locus_tag="ACHK49_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO9262985.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16802..17251)
                     /locus_tag="ACHK49_002347"
     CDS             complement(16802..17251)
                     /locus_tag="ACHK49_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO9262986.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17269..18051)
                     /gene="truC"
                     /locus_tag="ACHK49_002348"
     CDS             complement(17269..18051)
                     /gene="truC"
                     /locus_tag="ACHK49_002348"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO9262987.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18051..18380)
                     /locus_tag="ACHK49_002349"
     CDS             complement(18051..18380)
                     /locus_tag="ACHK49_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO9262988.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
BASE COUNT         4612 a         5107 c         4475 g         4659 t
ORIGIN      
        1 cactttcgtg tgttcctttg ttgcagccca accaactatt gtcccacaat tacagcaaca
       61 agttacggat cttacgagta gcttaaactc acaagaaaag aaagaactga cgcacaagtt
      121 agaatctatt ttcaataata cccaagtgca aattgctgta ttaattgttc ccacaaccaa
      181 agacgagaca attgaacaat atgctacaag agtttttgac aattggcgtt taggagatgc
      241 caaacgtaat gatgggatac tgatcattgt tgcctggtca gatcgtactg tccgcatcca
      301 ggtaggttat gggctggaag aaaaggtaac cgatgctctg gctggagata tcatccgtag
      361 caacatgata cccgcattta aacaacaaaa atttgctcag ggattagagc tagctataaa
      421 cgctttgaat aaccaactca cttcacaaca ccaatatccg actaatcctt cagaaagtga
      481 atcagcgtct tccagtgatc attattactt tgctattttt tgggtatttg cagtgatgtt
      541 cttccctttc tggttttttc atcaaggcag taatttttgt cgcgcatgta aaagtggagt
      601 ctatatttca gcaatctatc ttttagattt attcctgttc tccgataaaa ttttttccat
      661 tgctgtattt tccttctttt ttactttcac catatttatg gtctttacct gtttatgtgt
      721 acttcagaaa agagcatcag gtaaaagtta tcattcagac aacagcggtt ccggtggagg
      781 ctcaagtggc ggcggattta gcggcggtgg cggttcttct ggaggtggcg gcgcatccgg
      841 ccgctggtaa ctcaaccata aaaaatgcca gcccggaggc tggcattttt aaatcagata
      901 aagtcagtct tatgcctggc ctttgatctc tttacgaccg ttgtacggtg ctttttcgcc
      961 cagagcttct tcgatacgaa tcagctggtt gtatttagca acacggtcag aacggctcat
     1021 agaaccagtt ttgatctggc ctgcagcagt accaacagcc aggtcagcga tggtagcgtc
     1081 ttcagtttcg ccagaacggt gagagataac tgcagtgtag ccagcatctt tcgccatctt
     1141 gattgcagcc agagtttcgg tcagagaacc gatctggttg aatttgatca ggatggagtt
     1201 agcgatacct ttttcgatac cttctttcag gatcttggtg ttggttacga acaggtcgtc
     1261 accaaccagc tggattttgt cgcccagaac tttggtctgg tatgcgaaac cgtcccagtc
     1321 agattcgtcc agaccgtctt cgatagaaac gatcgggtac tgtttggtca gttcttccag
     1381 gaagtgagtg aattcttcag aggtgaacgc tttgttgcct tcgccagcca gaacgtattt
     1441 accgtctttg tagaattcag aagctgcgca gtccatcgcc agagtgatgt ctttgcccag
     1501 ttcataacca gcagctttaa cagcttcagc gataacagcc agagcttcag cgttggaacc
     1561 caggttcggc gcatagccac cttcgtcacc aactgcagtg ttcatgcctt tcgctttcag
     1621 aacttttgcc aggtgatgga aaacttcaga acccatgcgg atggcttctt tcacagtttt
     1681 cgcgccaacc ggctgaatca tgaattcctg gatatcaacg ttgttgtcag cgtgctcacc
     1741 accgttgatg atgttcatca tcggaaccgg catagagtat ttgcccggag taccgttcag
     1801 ttcagcgatg tgctcgtaca gcggcatacc tttagcagct gcagcagctt tggcgttagc
     1861 cagagataca gccaggattg cgttcgcgcc gaatttggat ttgttttcgg tgccgtccag
     1921 gtcgatcatg atcttgtcaa tgccagcctg atctttagca tctttgccaa tcagcgcctg
     1981 agcgatcggg ccgtttaccg cagcaacagc tttggttacg cctttaccca ggaaacggga
     2041 tttgtcgcca tcgcgcagtt ccagagcttc acgggaacca gtagaagcac ctgacggagc
     2101 agctgccata ccgacgaaac caccctccag atgtacttcg gcttcaacag tcgggttacc
     2161 acgggagtcg atgatttcac gaccgatgat ttttacgatt ttggacatta ggttttcctc
     2221 aagtcactag ttaaactgaa actccagaca aacaacgcgt acccagggta cgcgttgccg
     2281 ttctaacttt tttacttact tcgcctgacg tttctggaac tcgctggcgg ctttcacaaa
     2341 gcctgcaaac agcgggtgac catcacgtgg agtagaagta aactccggat ggaactggca
     2401 agccacgaac cacgggtgat tcggaacttc gatgatctcg accaactgat catccccgga
     2461 acggcccgca acgcgcagac ctgcatcttc aatctgtttc aacagcatgt tgttgacttc
     2521 gtaacggtga cgatgacgct caacaattgt cggcgcattg tacagctggc gaaccaggct
     2581 atcgtcaacc aactggcact gctgtgcgcc gagacgcatg gtaccgccga gatcgctctt
     2641 ctcgctacga acttcaacgt tgccgttttc atcgcgccac tcggtaatca gcgccacaac
     2701 cgggtactta cagtctggca caaattccgt agagttggcg ttctccatgt tggcaacatg
     2761 gcgagcgtaa tcaattaacg ccacctgcat acccaggcaa atgcccagat aaggaatatt
     2821 gttctcacgc gcaaaacgcg cggtcgtaat catgccttct acgccacgat agccgaaacc
     2881 gccaggtacg aggattgcgt ccagaccttt aaggatttca acgccgcgcg tttcaacatc
     2941 ttgtgaatcg atcagtttga tgttgacgct gacacgattc ttcagcccac cgtgtttcag
     3001 tgcttcgatc actgatttat aagcatccgg cagttcaatg tacttgccga ccataccgat
     3061 ggtgacttca cttaccgggt tcgcttcttc gaagataacc tgttcccatt cggacagatt
     3121 cgcttccggg cagtttaagc tgaatcgttt acaaatataa tcgtccagcc cctgagattt
     3181 caacaggccc ggaattttat agatggaatc gacgtctttc agagaaataa ccgctttttc
     3241 cggaacatta cagaacaatg caatcttcgc acgttcgttc gccggaacag cgcgatctga
     3301 acgacaaatc aggatgtcag gctggatacc gatggagagc agctctttta cagagtgctg
     3361 agtcggtttg gttttgactt caccagacgc tgccatgtac ggcaccagcg tcaggtgcat
     3421 aaacagagtg tgctcacggc caatttcaac agccatctgg cgaatcgctt cgaggaacgg
     3481 caaggattcg atatcaccta ctgtaccgcc gatttctacc agtactacgt catgaccttc
     3541 gccaccttcc agcacgcgct ctttgattgc gttagtgatg tgcggaataa cctgcacggt
     3601 tgcgccgagg tagtcaccgc ggcgttcttt acgcagaacg tcagagtaga tacgacccgt
     3661 ggtgaagttg ttgcggcggc tcattttggt acgaatgaaa cgctcgtagt gccccaggtc
     3721 caggtcggtt tcagcgccgt cttcagtaac gaacacttcc ccgtgttgga ttgggctcat
     3781 agtacctgga tcgacgttga tgtacggatc cagtttcatg atggtcacat tgaggccacg
     3841 ggcttcaaga atggctgcga gggaggctgc ggcaatgcct ttacccagag aggatacgac
     3901 cccgccggtc acaaaaatat agttcgttgt catgctgaac ctgagaagtt aggttgaaaa
     3961 gacgatggaa taaccaggac gggaaagcag tatacccgaa catgacctgt gccacaaact
     4021 ttcattatcc ctcctcttcg ccagcgcact attgaaatca ggagtgagaa aatagcccct
     4081 ttggggtaaa tgtttttgac gcaaatcaag cgcttgtcat ttaaaaaatg acacaaatgg
     4141 cgcttgaccg cgtaattccc ttagagatca atttcctgcc gttttacctg ttgccagact
     4201 tcttccattg tttcgaggtc aacacctgtc atttccagtc cacgcgcggc aacaatacgc
     4261 tccacttcgc gaaaacgacg ctcaaatttt tcgttcgctt tttgcaatgc gatttccgct
     4321 tttgtcccta aatgacgagc cagattgacc gtggcaaaca gcaggtcccc catttcctcc
     4381 tccagtttag cctggtcgac aacagcctgc cgcgcttcgt acatcacctc gtcgatctct
     4441 tcgtagactt tatcgactac cggaccaagc gtcgtccaat cgaagccaac gttggcgcaa
     4501 cgtttctgga ttttttgcgc acgcattaaa gccggtaaac tacgaggaat atcgtccagc
     4561 gccgaatgct gcgctttctg cgcgcgctct tcggttttga tttgctccca acgggcaagc
     4621 acttcactac tgttttcggc agaactatca gcaaaaacat gcggatggcg acgctctaat
     4681 ttatcgctaa tagcagcgca aatatcatta aagtcaaagc gcccttcttc ctgagccatt
     4741 tgcgcgtaaa acaccacctg gaatagcaga tcgcccagtt caccgcgaag atcgtcaaaa
     4801 tcttcacggg cgatggcgtc cagcacttcg taggtttctt caagggtgta aggcgcaatg
     4861 gtggcaaatg tctgctcttt atcccacggg cagccgtttt ccggatcgcg caggcgctgc
     4921 ataatagtga gcaaacggtc gatttgattc attgaattgt cctgaaaatt gcgggtctgt
     4981 caggtggaaa cctgtgacca gaatagaagt gagttagtaa cactacccaa tcagtacgtt
     5041 aattttggct ttaatgagtt gtaattcctc tggggcaact gttcctttct tcgttgctcc
     5101 tcttgcccgc caggcgatac tttttacctg atcagctaac gctacgccat cacgttcctg
     5161 accggataaa acaacttcga acggatatcc ttttgattgc gttgtataag gaacacacag
     5221 acacatacct gttttgttgt tgtacatgaa aggactcagg acaacagctg gacgatgtcc
     5281 agcttgctcg ctaccttttg tcgggtcaaa atcaacccaa atcagatcgc ccatatcggg
     5341 tacgtatcgg cttaccatta ccagacttcc ttatctttcg gctctcccca gtcgatattc
     5401 tcgtggaggt tttccggcgt gatgtcgttg accagttcag caagcgtaaa tacgggctct
     5461 ttacgcactg gctcaataat taatttgcca tccaccaggt caatcttcac ttcatcatca
     5521 atattcagat tgagcgcctg cattaacgta gccgggatcc gcaccgccgg tgaatttccc
     5581 caacgcttta cgctactgtg gatcataacc ctttcctcaa accgctatca tatgtagata
     5641 cagtatatat caatctacat tgtagatacg agcaaatttc ggcctaactc ccgtgcaacc
     5701 gacgcgcgtc gataacatcc ggcacctggt tgagtttacc cagcacgcgc cccagcactt
     5761 gcaggttgta aatctcaatg gtcatgtcga tggtcgccag ttgctgtttg gtgtcgctac
     5821 ggctggcaac gccaagcacg ttcaccttct cgttggcgag aatggtcgtg atatcacgta
     5881 acaacccact acgatcatta gccaccacgc ggaccaccag cgaatatccg gcggagtagc
     5941 tctcacccca taccgcgtca acaatgcgtt ctggcgcatg ggagcgcagt tccgccagtt
     6001 gttcgcaatc ggcgcggtgt actgaaatac cgcgcccctg ggtaatgaag ccgacaatct
     6061 catctccagg aatcggctgg cagcagcgcg cgatgtggtg catcaggttg ccaacacctt
     6121 cgaccaccac gcgaccgtta tctttactgc ggttttgcgg cgtgtagctt ttttgctgaa
     6181 gttgcttcag cgcggcggcg tcctgctctt cggcactcgg cttattaaat tgcgattgca
     6241 ggaagttcac catctgattg agacggatat ccccgccacc aatcgccgcc agcaactcgt
     6301 cgacatcatt aaagttgtaa cgcggcagca gatgtttttc tgcttctttc aggctgatcc
     6361 ccagatgttc cagctcgtcg tcaaggattt gccgcccagc cagaatgttt ttgtcacggt
     6421 cctgtttacg gaaccaggcg tgaattttcg aacgcccacg gctggttgtg acgtaaccga
     6481 ggtttgggtt taaccagtca cggctggggt tcggctgttt ctgggtgata atttcaatct
     6541 ggtcgcccat ctgcagctgg taggtgaacg gcacaatgcg cccgccaatt tttgccccga
     6601 tgcagcggtg tccgacatca ctgtggatgt ggtaagcgaa gtccagcggc gttgatcccg
     6661 caggcaaatc aacgacatca cctttcggcg taaagacata cacccggtcg tcaaagacct
     6721 ggctgcgtac ttcgtcgagc atttcgccgg aatcagccat ctcttcctgc cacgcaatca
     6781 gtttacgcag ccaggcaatc cggtcttcat gtcccgaacg tgcgccgcca gctgccgcgc
     6841 cctctttata tttccagtgc gcagcaacac ccaactctgc atcttcatgc atctgtttgg
     6901 tgcggatttg gatctcaacg gttttgccac ctggccccag aaccacggta tgaatagact
     6961 gataaccgtt tggtttcggg ttagcgacat aatcgtcaaa ctcatccggc aggtggcgat
     7021 agtgagtgtg cactatcccc agtgcggcat aacaatcctg taaacgctcg gcgacaatac
     7081 gtaccgcacg cacatcaaac agctcatcga aggcgaggtt ctttttctgc attttgcgcc
     7141 agatactgta gatgtgtttc ggacgaccat acacttcagc tttaacgcct tcagctttca
     7201 tctcagcgcg cagatggccg acaaactctt cgatatagtg ttcgcggtcg agacggcgtt
     7261 catgcagcag ttttgcgatg cgtttgtatt cggtcgggtg gagataacgg aagcagtaat
     7321 cttccagctc ccatttcagt tgcccaatcc caagacggtt cgccaacggc gcgtagatat
     7381 tggtgcactc ttttgccgcc agtacgcgtt catcttccgg cgcatctttt acttcgcgca
     7441 gatgagcaat acgctccgcc agtttgatga ccacgcagcg aaaatcatcg accatcgcca
     7501 ataacatccg gcgaacgtta tcgacctgtt cggaggaaac agaatcagtg tgcgtcgctt
     7561 tcagctggcg gatcgccgcc atatcacgca cgccgtgaat aaggttaacg accgacttac
     7621 cgacgctctc acgcagcaca tcttcgctga ctacgttggc atcagccaga gggaacagca
     7681 gcgccgcccg cagcgtgtca atgtccatac ttaatgtcga gaggatctcc accatctcaa
     7741 caccacgcca caataacaga ctggcatccg gatgcccctg cgtctgttgc agacaatacg
     7801 cccaggtttc ggctaagcac tcacacgact tctggctggt aatacccaga cttgcgatcc
     7861 atttttccgg atcaaattca ccagccttat tgatatgtgc acttcttacc gcaaccattg
     7921 tcctctcctt tagggaccag acctgccgaa atcggcaaat cgtaactatt taacgcgcga
     7981 gaaaagtacc atcgattcca gatgtcccgt gtgtgggaac atatccagca tcgccagtcg
     8041 cgcaatggta tatcctgctt ttaataacgc ttcgctatcc cgagccagcg ttgcagggtt
     8101 acaggataca taaactatac gaataggttc cagttttata atttgctgca taacacctgc
     8161 ggcacctgct cgcgccgggt ccagcaacac tttatcgaag ccgtttttcg cccacggctg
     8221 ctttgtgaca tcttcttcaa gattttcgtg ataaaacgtc acattctgta aaccattaag
     8281 acgcgcattc tgctggcctt tttccaccag cgccggaaca ccttctacac cgaccacact
     8341 ggctgcttgt ttcgccaatg gcagtgtaaa gttgcccata ccgcagaaca gatccagtac
     8401 gcgatcttca ggttgcacat ccagccattc caacgcacgc gctaccattt tttggttcac
     8461 acccgcattg acctgaataa aatcgcgcgg gctaaaagtt aagcgcaacc cgtttgagtc
     8521 ataccagggc atctcaccag agacggtttc gagtatctca ctatcggggg cgagatacag
     8581 atccaggcct tcagaatgcg aaaagcgttc cagtttttcg cgatctgccg aacttagcgg
     8641 tgcggtatgg cgcaaaatca tcagcgtgcc gctggttgcc tgtaccagtt caacatgacc
     8701 aagatggcgc ataacttgta agctgcccag gcatgccctg actttgggca gcaatgcttc
     8761 aagttggggc actaaaatgg ggcattgttt aacgtcgaca atgtcactgg agcccgcttt
     8821 gcgaaacccc atctgaagtt gctgtgtttt cggtaagtag ttcagactta aacgcgcgcg
     8881 gcggcgatag ccccagggaa catcggcgat cacttcagag acatcgtgtt tcattaatcg
     8941 ggcgagtgcc gcacttttgc ttcgctgctg taaatccacg ctggcgtgtt gttgctgaca
     9001 gccgccgcat acgccaaaat gaggacagcg tggtgtttcg cgttccgggc tatcgcttaa
     9061 ccggcgtacg actttagcgc gggcatactg ttttttatct tcagtaacag caacttccgc
     9121 gttttcctgc ggcaataatc cggggataaa taacgttttg ccgttatgtc gcgccacgcc
     9181 ctgaccaaaa gagtcgaggt cgttgactga aacggttatg atctgacgcg tcgtcgtgcg
     9241 tcgttttgca gagtagaatt gcgccattgg cgagactttc tcaatttaac agtgtgacct
     9301 taattgtccc ataacggaac tccatgacca actacagcct gcgcgcacgc atgatgattc
     9361 tgatcctggc accgaccgtc cttattggtt tattgctgag tatctttttc gtcgtgcatc
     9421 gctataacga cttgcagcgt caactggaag atgccggtgc cagcattatt gagccgcttg
     9481 cagtttctac tgaatatggc atgagcctgc aaaatcgcga atctatcggt cagttaataa
     9541 gcgtactgca tcgtcgccat tccgatattg ttcgcgcgat ttcggtttat gatgaaaata
     9601 accgactctt tgtcacctcc aattttcatc ttgatccctc atcaatgcag ctcggcagca
     9661 acgtgccgtt tcctcgccag ctcactgtca ctcgtgacgg cgatattatg atcctccgca
     9721 cgccgattat ttctgagagt tactcccccg acgaatcgcc cagtagcgat gccaaaaata
     9781 gtcaaaatat gttgggatat attgcgctgg agctggatct taaatcggtt cgcttgcagc
     9841 aatataaaga gatctttatt tccagcgtga tgatgctgtt ttgtatcggt attgcgctta
     9901 tttttggctg gcgcttaatg cgcgatgtaa ccggtccgat tcgcaacatg gtgaataccg
     9961 tcgaccgcat ccgtcgcggg caactcgaca gccgggtgga aggatttatg ctcggcgagc
    10021 tggatatgct gaaaaacggt atcaactcga tggcaatgtc gctggctgct tatcacgaag
    10081 agatgcagca caatatcgac caggcgacgt ccgatctgcg tgaaacgctg gagcagatgg
    10141 aaattcagaa cgttgagtta gatctggcga aaaagcgcgc ccaggaagcg gcgcgtatta
    10201 aatccgagtt tctggcaaat atgtcacacg agctgcgtac accactgaat ggtgttattg
    10261 gctttacccg cctgacgctg aaaacagaat taacaccaac gcagcgcgat cacctgaata
    10321 cgattgaacg ttcggcaaat aatttgctgg caattattaa tgatgttctc gacttctcga
    10381 aactggaagc aggtaagctg attctggaaa gtattccatt cccactacgc agcacgctgg
    10441 atgaagtcgt tactctgctg gcacattctt ctcacgataa agggttggaa ctgacgctca
    10501 atattaaaag cgacgtgcct gataacgtga tcggcgaccc actgcgatta cagcaaatca
    10561 tcactaacct ggtggggaat gcaattaaat tcaccgagaa tggcaacatt gatattctgg
    10621 tagaaaaacg tgcgctgagt aataccaaag tgcagattga agtgcagatt cgggataccg
    10681 gcattggtat tcctgaacgc gatcaatcgc gcttattcca ggccttccga caggctgatg
    10741 ccagtatttc ccgccgtcat ggtggcaccg gtctggggct ggtgattaca caaaaactgg
    10801 ttaatgaaat gggcggcgat atttcgttcc atagccagcc gaatcgcggt tcaactttct
    10861 ggttccacat taatctcgat ctgaacccga acattattat cgaagggcca tccacccagt
    10921 gcctcgccgg taagcgcctg gcctatgtcg aaccaaactc cgcagcagcg caatgcacgc
    10981 tggatatttt aagtgaaacg ccgctggaag tggtttatag cccaacgttc tccgcgctgc
    11041 ctcccgcgca ttacgacatg atgttgttag gcatcgcggt gaccttccgc gagccgctaa
    11101 caatgcaaca tgagcgatta gcgaaagcgg tatcgatgac cgatttcctg atgctggcac
    11161 ttccttgcca tgcacaagtc aatgcagaaa aactcaagca agatggtatc ggcgcgtgtc
    11221 tgctgaaacc attaacacct acgcgcctgt tgcctgccct gacggaattt tgtcatcaca
    11281 aacaaaacac gcttttgcct gtaaccgatg aaagcaagct ggcaatgaca gtcatggcag
    11341 ttgatgacaa ccccgctaac ctgaaactta tcggcgcatt gctggaagat atggtgcaac
    11401 atgtggaact ttgcgatagc gggcatcagg cggttgaacg ggcgaaacag atgccgttcg
    11461 atttgatctt aatggatatt caaatgcctg acatggatgg cattcgggcc tgcgagctca
    11521 tccaccagct cccccatcag caacaaacgc cggttatcgc ggtaacggcg catgcaatgg
    11581 ccgggcaaaa agagaagctg cttggcgcag ggatgagcga ttatctggcg aaaccgattg
    11641 aagaagagcg attgcataat ttgttgttgc gctacaagcc tggcagcggt atttcctctc
    11701 gcgtcgtgac gcccgaagtc aacgaaattg tggtgaaccc gaatgcgacc ctcgactggc
    11761 aactggcact acgccaggca gcaggaaaaa ccgatttagc gcgcgatatg ctgcaaatgt
    11821 tactcgattt cctgcctgaa gttcgcaaca aagttgagga acagctggtt ggagaaaacc
    11881 cggaaggcct ggttgatttg attcataaac tgcatggcag ttgcggctat agcggtgtgc
    11941 cgcgtatgaa gaatctctgc caactgatcg aacaacagct acgtagcggt actaaagaag
    12001 aagatttgga gccggagctg ctggaactgt tggacgagat ggataatgta gcgcgcgaag
    12061 ccagcaaaat tctcgggtaa cggacgtctt gtcgagtagc aatcggcttt ttatgacgct
    12121 ggtttcagat tggtgacaaa gtgcctgtgt ttatgccgga tgcggcgtta acgccttatc
    12181 cggcctacaa aaccaattaa attcaatgaa ttaaaaaatg atgtagacct gatacatagc
    12241 acaacaggct attttgcgtt tagcatcagt ctcaagccgg ctccagatag agccggtttt
    12301 ggttttctgt cttaacgcac catgcacggg cgcttgttat cgaacgtcca gccaggaatc
    12361 agatactgca ttcccatcgc atcgtcacgc gcgccaagcc cgtgtttctg atacagctca
    12421 tgggctttca tcacttgatc catatcgatt tctacaccca gccccggttt ttccggcacc
    12481 tgaaccagac cgcctttgat ctcaaacggt tctttggtca ggcgctgatt accttcctgc
    12541 cagatccagt gcgtatcaat agcagtaatt ttacccggtg cagcggcggc aacatgggta
    12601 aacatcgcca gggaaatatc gaagtggttg tttgagtgtg aaccccaggt caggccaaat
    12661 tcatggcaca tttgcgccac acgtaccgaa ccttgcatcg tccagaaatg cggatccgcc
    12721 agcgggatat caacggattg cagggagagc gtatggccca tctgccgcca gtcggtggcg
    12781 atcatattgg ttgcggtcgg caggccagtt gcgcgacgga actcagccat cacttcacgc
    12841 ccggagaaac cttgctccgc accacacgga tcttctgcat aagccagcga ccctttcagg
    12901 tatttaccaa ttttaatcgc ttcgttcagc gaccaggcac cgttaggatc gagcgtaata
    12961 cgcgcctgcg ggaagcgttg cgccagtgcc acaatagact cggcctcttc ttccccggcc
    13021 agtacaccgc ctttcagttt gaaatcgttg aagccatatt tttcatacgc cgcttccgcc
    13081 aggcgcacca ccgcatccgg cgtcatcgct tcttcatgac gcaggcgata ccagtcgcat
    13141 gagtcatccg gctggctttg atacggcagc ggtgtggctt tgcgatcacc gacgaagaac
    13201 agataaccga gcatttcgac ttcgctacgc tgttgaccat cgcccagcag cgatgccacg
    13261 tttaccccca gatgctgccc cagcagatcc agcattgccg cttctatccc ggtaactaca
    13321 tgaatagtgg tacgcaaatc aaatgtctgc aaaccgcgcc caccagcatc acgatcggca
    13381 aaagtattac gcaccagcgt cagaacgttt ttgtatttac ccagcgtttt acctaccacc
    13441 agcggaatcg catcttccag cgttttacgg attttctcgc cgccgggaat ttcccctacg
    13501 ccagtgtgac cagaattatc tttgataatc acaatattac gcgtaaagaa cggtgcgtgt
    13561 gcaccactca gattcatcag catactgtca tgacccgcca ccgggatgac ctgcatttca
    13621 gtaacaacag gcgtcgtaaa ttgagaactc atatttatgt cctttttcag aatcaatgac
    13681 ggccgaaaac gggacgttta cggtcaaagg tccagccggg gatcaggtac tgcatcggac
    13741 ctgcgtcgtt acgcgcaccg ccaggcagac gtttataggc ctcatgtgcc ttttgtacct
    13801 gttcccagtc cagttccacg cccagaccgg gcgcatcagg aacggcaatt tttccgtttt
    13861 taatctccag cggatttttg gtcaggcgac aatcgccctc ctgccaaatc cagtgggtat
    13921 cgatagcggt aggattaccc ggtgccgccg cgcccacatg ggtaaacatc gccagagaga
    13981 tatcgaaatg gttattagaa tggcagcccc aggtcagccc ccagtcgtcg caaagctgcg
    14041 ccacacggac tgcaccggaa agcgtccaga agtgcggatc ggcaagtgga atatctaccg
    14101 cattgagcat caccgcatga cccatttcgc gccagttggt ggcgatcatg ttagtcgcga
    14161 cgggcaagcc ggtcgcccgc cgaaactccg ccatcacttc acgaccggag aaaccctgtt
    14221 ctgcgccgca tggatcttcg gcataggtaa gaacatcatt cagccctttg catagagaaa
    14281 tggcttcatc aagtagccag gcaccgttgg gatcaacggt aatccgcgca tcagggaagc
    14341 gtttcttcaa tgcccgaaca gtgtcgattt cttgctcgcc aggtaacacc ccgcccttaa
    14401 gtttgaaatc tttaaagcca tagcgatcct gtgaggcttc cgccagacgc acaacggctt
    14461 cgctgttcat cgctttctga tggcgcaact gataccactc atggttgccc ggcgtatttt
    14521 ccagataagg aagatcggtt ttggtccgat caccgatata aaacagataa ccgaggacgg
    14581 taatagtctc gcgttgcttg cctggcccca acagttcgca gaccggaaca ttcagcgcct
    14641 tacctagcag gtcaagcaag gcggcttcca gcgccgccac ggcattaacg cgcaattcaa
    14701 aagtccaggc acctttgccg aaggtatcaa aatcggctgc ctgattacct ttatgcacct
    14761 gctgaaccac tttattcagg cgcgcaactt cctggcccag aaccatcgga atagcatcga
    14821 ccagtgtttg ataaatcacc tctccgcccg gcgcttcacc aatgccggta tgcccggcgt
    14881 tatcggtgag taccacaata ttgcgggtga aatatgcgtt atgtgcccca ccaatattaa
    14941 gcaacatgct atcgtgcccg gcgaccggaa tgactttcat atcagtaata acaggactgg
    15001 attgtgtcgt catcttattg ccccgcgaca ggtttcagct cgatacgctt gatatcgccc
    15061 accagcacca ggtagctcag taccgcgatt aaggcatgaa caccaacata aatcagcgcc
    15121 ccattaaagg agccagtcgt gccaacgata taaccaattg cgattggcgt gacgatgcca
    15181 gaaatgttac cgaacatgtt gaacaggcca ccggaaagac cgctgatctc ttttggcgcg
    15241 gtatctgcca ttactgccca acccagcgcc ccgatgccct taccgaagaa ggccagcgcc
    15301 ataaagccga tgatcatcca ctcaacgttg acgtagttgc agaacaccat caccatcgac
    15361 agcaacatgc ccattacgat cggtgtttta cgcgcaatgt tcagcgatcc cgtgcggcgc
    15421 atcagccagt cggaaataat cccacccagc acaccgccga taaaaccgca aaccgccgga
    15481 acggaagcca caaagcccgc tttcagaatc gacatcccac gcgcctgcac cagataaacc
    15541 ggaaaccagg taatgaaaaa gtaagtcagg gcgttgatac agtactgacc gatataaacg
    15601 ccgatcatca tccgtgaccc gagcagttgt ttgatctgcc cccacttcac gctgaacggc
    15661 actttaactt tggtgttttg ctgatccata ttgatcagcg cgccacccgc ggcgatgtac
    15721 tccagctctt tctgatttac ccccggatgt tgatttggct cgtggatgac tttcaaccag
    15781 ataaagctga tgacaatccc cagaccgccc ataaagaaga agacgtgtga ccagcccact
    15841 tcatgcgtca gccagcccat aatcggcgcg aagatcaccg ttgcgaagta ttgagcggag
    15901 ttaaaaatcg acaccgccgt tcccctttcc tgcgccggaa accaggccgc aacaatgcga
    15961 ctgttgccgg ggaaagatgg cgcttcagca agcccgacca ggaagcgcag cgtaaacagg
    16021 gcaacgataa tgccgaatcc actaaagata tcgacgaagc cttgcagcaa ggtaaacatc
    16081 gaccagataa agatcgacca gaagtagacg cgttttgaac caaaacgatc cagcaaccaa
    16141 ccaccaggga tctgcccgat aacataagcc catgagaagg cagagaacac atagcccatt
    16201 cccacgggat caaggccgat atctttggcc atttccgaac cagcaataga aagcgtagcg
    16261 cggtcgccgt agttgaagga tgtgacgata aacaacatca ccactatcca gtaacgagca
    16321 ttcgtgcgtt tttccacact gctcgcagcc tgacttaaag aactcattgt tgcactcctg
    16381 aaaattcgcg ttagccacgc tcactctgga ctgcgacatc gccaggaaat cagaggtgac
    16441 gtagggtgtt ttttgccgtt tttataggtc gttcgccgaa tacggcgcgt gtttatatct
    16501 ggcaatagca gtataaaaag cgcgccatag cggctcaccg tgcaacaaca caacattaat
    16561 gcgttcaatg aggcccgatt ttggcattag ccctggacgg tggaatccac ttcacggaaa
    16621 tgaaaacaag aacaagaaag gaagggtaaa aacgaagaaa taaagaagag tatgaaatgg
    16681 atcgcttgac tccaggcaaa cgccagtaaa aatccgcgct atgaagcagt ttttactggc
    16741 atttgcctga aaagattcga ttcagcaccg ctaaaacgac atttaccgct cgctgaacat
    16801 atcaggacaa cagcgtgccc cactgttcga cccacggatt tgattccgtt tccggttccg
    16861 ggttctcact ggcgtcaatc aacagtattt cgccaacccg ctgagcgctc tgttcctgca
    16921 acaaggcatc gaactgtttg ccgccattgc agaaatttac ataactgctg tcgccgagcg
    16981 caatcacgcc ataacgcaga ttcggctgga agcccagact atctttgatt ccctgaaaga
    17041 gcggcacaat gctatcagga aggtcgccct gcccggtcgt ggacgtaacc accagaacat
    17101 acttatcctg atagggcagc cagtcgctta attcaggatc ttcaaatacc gttgctttgt
    17161 ggccctgtgc ggtcagaatt gcttctgctt cttccgccac taacagcgaa tttccgtaca
    17221 tggtgccgac aaaaatacca atttccgcca taccgttttc tccctggatt aggaacttat
    17281 ctctccatcc tgacccgatg gcgcactgaa ctcaaccctt tcattttcag gaagcagacc
    17341 gcgccagcca aattgtgata acgcctgcat ccaggtgtcg tccaggcccg cgtgaatagt
    17401 cagcggctcg ccagtaaaag gatgcgtcaa tgacaactga ctggcatgta gcattaaccg
    17461 ctggaggcca aaatgctcag caccgctgcg attctggcgt aaatcgccat gtttgctatc
    17521 gccaataatc ggatgacgca aatgggcaag atgtcggcga agctgatgtt tgcgtccggt
    17581 tttcggctcc agttccacca ggccgtagcg cgtggtcggg taacgtccgg tcgctaccgg
    17641 catttctacg gtcgccagac cgcgataatg cgtcactgct ggctgcgagc ctttatcttc
    17701 gcgggcaaat ttatcagcga ttttgtccag ttcttccacc agtggataat ccagaactgc
    17761 ttcttccatc aaccagccgc gcacaatcgc atggtaacgt ttctggattt ggtgctgttc
    17821 aaactgttgt gccagcagcc gtccggcctc gctggataat cccatcaaca acacaccaga
    17881 agtgggtcgg tccagacgat gagcggtaaa aacgtgctgg ccaatctgat cacgcacggt
    17941 ttgcatgacc actactttct cgtcgcgatc cagccagctg cggtgaacca gccagccgga
    18001 gggtttattt accgcaacca gccattcatc ctgatagagt atttccagca ttagctcgca
    18061 tcatccgcaa aaagagcatc cagtttttcc agctcagcca gaataagcgc gcgttgcgga
    18121 tggtccgtcg ccagcgccat ttcataatag ggtgcaacgg caaaagcgcc cggtaacggc
    18181 tgtttgttat ctaacaaatc gtgcattcgc gggatcagca cccactgcaa ccactccagt
    18241 ggttccatgg tgtccataaa gaacggttgg gtactattaa attgatgcgg ctggggttca
    18301 tcgtttcgcc agtgctgatg ttcacgcagt aacgcttcaa gcgcctgcaa ctggagacga
    18361 acgcggtcat gagtggtcat aaagcaacct caataagaaa aactgccgcg aaggatagca
    18421 ggaataaaaa aagggagcac tgtattcaca gcgctcccgg ttcgtttcgc agcattccag
    18481 ctacttttgt tgctccctgc tcatccttga caacttttcc tctggccttg cggccaatcg
    18541 ttcatcctga actattgctt cctgctcaca ccaccccgat gtgatacttc atcctgaagt
    18601 gtccctggcc ttcctgaccc accgaatcat cctgaccggt tctcattctc catcctggag
    18661 gtgtccttta acgcgtcctg cgtcatcctc ttcgcttcat ccagaagcct ttccctgaaa
    18721 caccatcctg gtgtgtcctg cagaagtgtc atcatcctga tgttcacttc gttgtctgac
    18781 tccctgtcga cgaagataga atcgtctttt tcgcgaagtc ttacaaggcg cttacagaca
    18841 atgccttaag gaa
//
LOCUS       DAZGLJ010000076        18754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000076 DAZGLJ010000000
VERSION     DAZGLJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.076"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            52..1005
                     /gene="ybhD"
                     /locus_tag="ACHK49_002350"
     CDS             52..1005
                     /gene="ybhD"
                     /locus_tag="ACHK49_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9262989.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1046..2041)
                     /gene="pgl"
                     /locus_tag="ACHK49_002351"
     CDS             complement(1046..2041)
                     /gene="pgl"
                     /locus_tag="ACHK49_002351"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO9262990.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYSIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2196..3014
                     /gene="ybhA"
                     /locus_tag="ACHK49_002352"
     CDS             2196..3014
                     /gene="ybhA"
                     /locus_tag="ACHK49_002352"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO9262991.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(3015..4073)
                     /gene="modC"
                     /locus_tag="ACHK49_002353"
     CDS             complement(3015..4073)
                     /gene="modC"
                     /locus_tag="ACHK49_002353"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO9262992.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4076..4765)
                     /gene="modB"
                     /locus_tag="ACHK49_002354"
     CDS             complement(4076..4765)
                     /gene="modB"
                     /locus_tag="ACHK49_002354"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO9262993.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4765..5538)
                     /gene="modA"
                     /locus_tag="ACHK49_002355"
     CDS             complement(4765..5538)
                     /gene="modA"
                     /locus_tag="ACHK49_002355"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9262994.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEEHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5704..5853)
                     /gene="acrZ"
                     /locus_tag="ACHK49_002356"
     CDS             complement(5704..5853)
                     /gene="acrZ"
                     /locus_tag="ACHK49_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO9262995.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            5982..6770
                     /gene="modE"
                     /locus_tag="ACHK49_002357"
     CDS             5982..6770
                     /gene="modE"
                     /locus_tag="ACHK49_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO9262996.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6838..8310
                     /gene="modF"
                     /locus_tag="ACHK49_002358"
     CDS             6838..8310
                     /gene="modF"
                     /locus_tag="ACHK49_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO9262997.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            8571..9587
                     /gene="galE"
                     /locus_tag="ACHK49_002359"
     CDS             8571..9587
                     /gene="galE"
                     /locus_tag="ACHK49_002359"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO9262998.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9597..10643
                     /gene="galT"
                     /locus_tag="ACHK49_002360"
     CDS             9597..10643
                     /gene="galT"
                     /locus_tag="ACHK49_002360"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO9262999.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTRTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10647..11795
                     /gene="galK"
                     /locus_tag="ACHK49_002361"
     CDS             10647..11795
                     /gene="galK"
                     /locus_tag="ACHK49_002361"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO9263000.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYYLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11789..12829
                     /gene="galM"
                     /locus_tag="ACHK49_002362"
     CDS             11789..12829
                     /gene="galM"
                     /locus_tag="ACHK49_002362"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO9263001.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            13032..13784
                     /gene="gpmA"
                     /locus_tag="ACHK49_002363"
     CDS             13032..13784
                     /gene="gpmA"
                     /locus_tag="ACHK49_002363"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO9263002.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13943..14995)
                     /gene="aroG"
                     /locus_tag="ACHK49_002364"
     CDS             complement(13943..14995)
                     /gene="aroG"
                     /locus_tag="ACHK49_002364"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO9263003.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGETAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            15311..15691
                     /gene="ybgS"
                     /locus_tag="ACHK49_002365"
     CDS             15311..15691
                     /gene="ybgS"
                     /locus_tag="ACHK49_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO9263004.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15805..16746
                     /gene="zitB"
                     /locus_tag="ACHK49_002366"
     CDS             15805..16746
                     /gene="zitB"
                     /locus_tag="ACHK49_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO9263005.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16743..17462)
                     /gene="pnuC"
                     /locus_tag="ACHK49_002367"
     CDS             complement(16743..17462)
                     /gene="pnuC"
                     /locus_tag="ACHK49_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO9263006.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRLAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17500..18543)
                     /gene="nadA"
                     /locus_tag="ACHK49_002368"
     CDS             complement(17500..18543)
                     /gene="nadA"
                     /locus_tag="ACHK49_002368"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO9263007.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
BASE COUNT         4703 a         4900 c         4695 g         4456 t
ORIGIN      
        1 tttttaacga ccagccaccc aaaatcgtcg cttccatttt ccggagtaat aatgaagcat
       61 gaattatcaa gtatgaaggc atttgtcata ctggcagagt ccagttcatt taacaatgct
      121 gctaaattac tcaatattac gcaacctgca ttaacgcgaa gaataaaaaa gatggaggaa
      181 gatttacata ttcaactatt tgagcgtaca actcgcaagg ttacgttaac aaaagcagga
      241 aaaaggttgc tcccggaggc gcgggaatta ataaagaaat tcgatgaaac gctttttaat
      301 attcgtgata tgaatgctta tcatcgtggt atggtgacgt tagcgtgtat tccgaccgca
      361 gtattttatt ttttaccgct ggcaatcggt aaatttaatg agctgtatcc caatattaaa
      421 gtgcggattc tggaacaagg cacgaataat tgcatggaat cggtgctgtg taacgaatca
      481 gactttggca taaacatgaa caacgtcaca aattcatcca ttgattttac cccactggtc
      541 aacgagccat tcgtgctggc ctgtcggcgt gaccatccat tggccaaaaa gcagcttgta
      601 gaatggcagg agttagtggg ttacaaaatg attggcgtac gttcctccag cggcaaccga
      661 ttgttgatag aacaacagct agccgacaag ccctggaagc tggactggtt ttacgaagtg
      721 cgtcatcttt cgacgtcatt aggactggta gaggcgggac tggggatttc ggcgcttcct
      781 ggtctggcaa tgccccatgc gccctattct tcgattattg gtattccact ggtggaaccc
      841 gttatacgtc ggacattggg gattattcgc cgcaaagatg ccgtgctttc tccggcagca
      901 gaacgctttt ttgccttgct gattaatttg tggactgacg ataaagacaa tttgtggacc
      961 aatatcgtcg aacgccagag acatgcgtta caggagatag gttaatacac cggcgcagga
     1021 gagactgcgc cggttaaatc agcggttagt gtgcgttaac caccacccac attggtccct
     1081 gcccgaccgc ataacggcct ttttcatgca gtagtccctg ctcgccaaca atttcgtata
     1141 ccgagatgtg gtgagatttt tgcccggcgg caatcagata cttgccgctg tgatcaacat
     1201 tgaagccgcg cggctgggtt tccgttggct ggaagccttc tttactcaac acgctgccat
     1261 cttccgaaac gctgaaaacg gtaatcaggc tggcggtacg gtcgcaggcg tataaatggc
     1321 gaccatccgg ggtgatatga atatcagccg cccaacgggt gtcggagaag ttttccggca
     1381 tcatatccag cgtctggaca cattcgatat taccgtgcgg atctttcagt tcccagacat
     1441 ccactgagct gtttaactca ttgacgcaat acgcatattg ttcgtttgga tggaatacca
     1501 tatgacgcgg gccggcccct tcaacggtgg tcacttccgc agggtcctgc gccacgagat
     1561 gaccatcatc gctgaccgta aacaggcaaa tgcgatcctg ctttaatgcc ggaacccaca
     1621 gcgtacggtt gtccggtgag atattggcgg aatggcaacc gtccagcccc tcgaccacat
     1681 cgacgacgcc cactggcagg ccatcttcca gacgcgttac gctcacgtta cccgcattgt
     1741 aagaacctac aaagacaaac tgcccctggt gatcggtgga aatatgcgtc ggactacccg
     1801 gcagcgcaga ctctgcggca aaggtcagtg cgccatcgtc cggggcgata ctatacgcca
     1861 ggacgcgaaa ctcagggcga acaccaacat agagataacg tttgtccggg ctgaccacca
     1921 tcggctgcac ctgccccggc acatcgacaa cctgtgtcag cgtcagtgcg ccttcatgat
     1981 tcaggttcca gacgtgaatt tgctggctct cagggctggc gatataaact gtttgcttca
     2041 tgaatgctcc tttgcattta gcaactgact gtaacagcta aaattagtcg cttttggcgg
     2101 tgaatgctga attaaaacgc agaattttgt ccgatagtgg tatcggtgta ccattcgcgc
     2161 agacgaaaat tcttaacctt aatctggaat acgccatgac cacacgcgtg attgctctcg
     2221 acttagacgg caccttattg accccgaaaa agaccctgct tccttcatcg atagaagccc
     2281 tggcccgcgc tcgcgaagca ggctatcaat taatcatcgt cacaggtcgc catcacgtcg
     2341 ctattcatcc tttttatcag gcgctggcgc tggatacacc tgctatttgc tgtaatggca
     2401 cctatttgta tgattatcat gcaaaaaccg tgctggaagc ggacccaatg cccgttaata
     2461 aagccctgca actcattgag atgctgaatg aacaccacat tcacggtctg atgtatgtgg
     2521 atgatgcaat ggtctatgag cacccgaccg ggcatgtcat tcgcacgtct aactgggcgc
     2581 aaaccctgcc gccggaacag cgtccgactt tcacacaagt cgcttctctg gctgaaacgg
     2641 cgcaacaagt taacgccgta tggaagttcg ccctgacgca cgatgacctg ccgcaattgc
     2701 agcattttgg taagcatgtc gaacatgaac tgggactgga gtgtgaatgg tcctggcacg
     2761 atcaggttga tattgcacgc ggcggcaaca gcaaaggtaa acgtttgacg aaatgggttg
     2821 aggcgcaagg ctggtcgatg gaaaacgtcg tggcgttcgg cgataacttt aatgatatca
     2881 gtatgctgga ggccgctggt acaggcgtgg cgatgggcaa cgccgatgac gcggtaaaag
     2941 cgcgcgccaa cattgtgatt ggtgataaca ccaccgacag cattgcccag ttcatttata
     3001 gccacctgat ttaatcaggc ggttatcgac acacttttaa tttgcgcgta cagccacagg
     3061 ccaggtttga tcaccagttc atccctggcc cacgggctga tacgcgccca cagcgtttta
     3121 cctccgactt ccagttccac ttccacctgg ccgttgtcgt cataactatt aacaactttt
     3181 gcccgcaata cgttacgaat gctggtttgc tgcggcggtt gtaaaaccag agaaacatcg
     3241 gaagcctgaa tgcggatacg tagcgcagct tgcagcggtt cgtccagctt attgacccac
     3301 aaatgctgat cgcccagcgc cagcgcagtc atcgcgtaat gcggatgatg ctcaagtacc
     3361 gtcactttca gaatgctact ttgttgctct ttcggcagcc acggattcat cacgctacta
     3421 ccccagactt cttccagtgc gccaaaggct ttcacctgac cgttttccag taccatcact
     3481 ctgtctgcca gatggaggat ctcatccagc gaatggctga catacaacat cgggatgttg
     3541 atttcccgcg tcagacgttg cagataaggc aacagttcgc gtttacgcgg aatatccagt
     3601 gacgccagcg gttcatccag caacagcaat tctggtgccg tcagcaaagc ccgcccaatc
     3661 gctacgcgct gtttttcgcc tccggacagg ctgcctggta aacggtcaag caacggttca
     3721 atgcctaaaa gcgccaccag cttatcgaac tgatcgacca tgcttttcgc catgccgtag
     3781 cgcagattgc cacgcacttt gtaatgcgga aacagccgcg catcctgaaa aacatagcca
     3841 acgcgacgct tttctggcgt caggcagata cctttttcgg catcatttag tacccgacca
     3901 ttgaggacaa tcctcccttt ttgcgggcgc gtcagtccac tgatggcgtt aatcagcgaa
     3961 gtttttccgg caccggagac gccaaagata gcagtgatgc cattggcggg cagcgtttca
     4021 ttaatggtca ggcaatggtt gcccaaggtc tgggaaaaat tcagttccag catgattagc
     4081 gccccgcccg ttcacggctg attctggcca gccattctga aatcaacagg gagatcatcg
     4141 ccagcgcaat agaaataatg cacagtctcg ccgcgccact ttccccgccg ggggtctgga
     4201 tcagggtata catggcagaa ggaatggttc gcgtttcacc aggaatgttc gacacaaagg
     4261 tgatggttgc accaaactca ccgagagaac gagcaaaagc cagtaccgta ccaacaataa
     4321 ttcccggtaa ggtcagcggt aacgtgatag taaagaaaac gcgccagcgc ccggccccca
     4381 gtgttcttgc ggcctgttcg agtttgacat caaccccttc cagcgccaga cgaattgccc
     4441 gcaccatcag cggaaacgac atgacggcgg cagcgagaac cgcgccgcgc cagctaaagg
     4501 cgaaggtaat accaaaccag tcatacagac gttcaccgat aaatccgcgc cgtcccatcg
     4561 aaactaataa taagtaaccg acgaccacgg gcggtaacac cagcggtaga tgcagtacgc
     4621 tgtcgagcag agctttgccc ggaaacgtgc aacgcaccag taaccaggca aaaaagatcc
     4681 caaacggcag gctaaacagc acagccaggg aagaaacttt caggcttaat aaaactgcct
     4741 gccattctgg atcggtcagt atcattactt ggttgtaaat ccgtaacgtt taaagatttc
     4801 cgcagcctgc ggccctttca aataatcata aaatgctttc actgtcgcat tgttatgctc
     4861 ttccacaaca gcaaccggat attccacttt tttatgtgaa tcttccggga aggtggcaac
     4921 cacttttacc cctttgctgg caactgcgtc agaaccgtaa acaatgccca acggcgcttc
     4981 attgcgttcg accaacgcca gcgcgccacg aacatcttcc gccggagcca gtttcgggga
     5041 gagcgtatcc catgcgccca gtttttgcag tgcttctttc gcataaatgc cagcagggac
     5101 atgttccgga tcgccaaccg cgaggcgacc gccattcagc agcgaagtcc agttggtttt
     5161 gctgtcgatg gtgaaatctt tctgctcgct ggctttcggt gctacaacga ccaggctatt
     5221 gccgagcagt gtctggcgcg aagccgtatc gatcgctttt ttatcaaccg cataatccat
     5281 ccatttctga tcggcagaga taaacagatc cgcaggcgct cccgcttcaa tctgacgggc
     5341 cagagtagat gacgaagcga aagaagaaac cacatccacg cctttttctt ttttatactg
     5401 cgtagcaatg tcctgcattg cgttagtcag tgatgcggcg gcgaacaccg tgattttccc
     5461 ttcatctgcc agtgtattgc cagcaacagc gaaagagagt gccgccccgg caaacaagtt
     5521 caaccattta cgagccattg gtaacccctt aatgtaacgt tatgtaggcg acaatataac
     5581 ggctagctca gaattttccc agcagttatt gctaaccttt gtgaggtaga taagaaaaaa
     5641 tatcggctgg aaaagcagga agttgagaaa aagaaatgcc cgactaagcg ggcattcagg
     5701 gaatcaatga ttttgtccgg gctggtcttt tttaccaaca ccagaaaaga tgttgaatac
     5761 ttcaccaaga ccgtaaatca gacccaggat gatggccatc acgacaggta ccatgattac
     5821 ggcgaatacc agacttttta ataactctaa catggtcaac tccagatata gtcatgagac
     5881 tattctaacc gctaagcaca gaaaagcact ccccttttgt gcggtcagct ttgcgtggcg
     5941 ttcgttttcc gtcacaataa gacttttgcc aggacattgt tatgcaggcc gaaatccttc
     6001 tcacccttaa gctccaacaa aaattattcg ccgacccgcg ccggatttcg ctactaaaac
     6061 acattgcgct ttccggttcc attagccagg gagcgaaaga tgccggtatc agctataaaa
     6121 gcgcctggga tgccattaac gagatgaatc agttaagtga gcatattctg gtcgagcgcg
     6181 caacaggcgg taaaggtggc ggcggcgcag tactgacccg ctatggtcag cgactgattc
     6241 agctctatga cttactggcg caaatccagc aaaaagcctt tgatgtgtta agtgacgatg
     6301 acgccctgcc gctgaacagc ctgctggccg cgatctcacg tttttcactg caaaccagcg
     6361 cccgtaacca gtggttcggc accatcaccg cccgcgatca tgatgacgtt caacagcatg
     6421 ttgatgtctt actggctgac ggaaaaacac gcctgaaagt cgcaattacc gcacaaagcg
     6481 gcgcgcgtct ggggctggat gaaggcaaag aagtgttgat attgctaaaa gcgccgtggg
     6541 taggtattac tcaggacgag gcggtcgcac aaaacgccga caaccaatta ccgggtatta
     6601 ttagtcatat tgagcgcggc gcagagcagt gcgaagtatt aatggcgcta cccgacgggc
     6661 aaacactgtg cgccacagtg ccggtaaatg aagcgacttc tcttcagcaa gggcagaatg
     6721 tcacggccta ctttaatgcc gacagcgtga ttatcgccac gctgtgctaa gcgtgttgac
     6781 aatttgttat gaaacacgta tccctgtcag taatcgctgc acaaaatggg atataaaatg
     6841 tcatcgttgc aaattttgca aggcacgttt cgtcttagcg acacaaaaac gctgcaattg
     6901 cctcagctaa cgttaaacgc gggtgatagt tgggcgtttg tcggttcgaa tggaagcggg
     6961 aaatcggccc tggcccgcgc gctggcgggg gaacttccgc ttttgaaagg tgaacggcaa
     7021 agccagtttt cccacatcac tcgtctctcc ttcgagcaat tgcaaaaact cgtcagcgac
     7081 gaatggcagc ggaataacac cgatatgctc ggccctggcg aagatgacac cggacgcact
     7141 acggctgaga tcattcagga tgaagtaaag gatgcaccgc gttgcatgca actggcgcag
     7201 cagttcggta ttaccgccct cctcgaccga cgctttaaat acctttccac tggcgagacg
     7261 cgaaaaaccc tgctgtgtca ggcgctgatg tcggagcctg acttgttgat tcttgatgag
     7321 ccgttcgatg gcctggatgt tgcctcacgt cagcagctgg ctgagcgact cgcctcgtta
     7381 catcagtccg gtattactct ggtactggtg ctcaatcgct tcgatgagat cccggagttt
     7441 gtccagtttg ctggcgtgct ggcggattgc acgttagcgg aaactggcgc taaagaggaa
     7501 ctgctccaac aagcactcgt cgcgcaactg gcgcatagtg aacagcttga aggtgtgcaa
     7561 ctgccggagc cggatgaacc ttcagcacgt cacgccttac ccgccaacga accgcgcatt
     7621 gtgctgaaca atggcgtggt ttcttataac gatcgcccca ttcttaataa ccttagctgg
     7681 caggtgaatc caggtgaaca ctggcaaatt gtcgggccaa atggtgcagg aaaatcgacg
     7741 ttattaagcc tggttactgg cgatcatccg caaggttaca gcaacgattt gacgcttttc
     7801 ggacgacgtc gcggcagcgg cgaaaccatc tgggatatca aaaagcatat cggttacgtc
     7861 agcagtagtt tgcatctgga ttaccgggtc agcactaccg tgcgtaatgt gattctttct
     7921 ggctattttg attcgattgg catttatcag gccgtttcgg atcgccagca aaaactggtg
     7981 cagcagtggc tggatattct cggcattgat aaacgtacgg ctgacgctcc gttccatagt
     8041 ctttcctggg gacagcagcg tctggcgctg atcgtccgcg cactggtgaa acatccgacg
     8101 ttgcttattc tcgatgaacc actacagggg cttgatccgc tcaatcgcca gcttatccgc
     8161 cgttttgttg atgtgctgat tagcgaaggt gaaacgcaat tgttgtttgt ttcgcaccac
     8221 gctgaagatg cgcctgcctg tattacccat cgccttgagt tcgtgccgga cggtggactc
     8281 tatcgctatg tgctgacaaa aatatattga gtcggtagtg ctgaccttgc cggaggcggc
     8341 ctaagcgccc tctccggcca acggttcgac gcatgcaggc atgaaaccgc ttcttttttc
     8401 agataaaaag cgcaatcatt cataaaccct ctgttttata atcacttaat cgcgcataaa
     8461 aaacggctaa attcttgtgt aaacgattcc actaatttat tccatgtcac acttttcgca
     8521 tctttgttat gctatggtta tttcatacca taagcctaat ggagcgaatt atgagagttc
     8581 tggttaccgg tggtagcggt tacattggaa gtcatacctg tgtgcaatta ctgcaaaacg
     8641 gtcatgatgt catcattctt gataacctct gtaacagtaa gcgcagcgta ctgcctgtta
     8701 tcgagcgttt aggcggcaaa catccaacgt ttgttgaagg cgatattcgt aacgaagcgt
     8761 tgatgaccga gatactgcac gatcacgcta tcgacaccgt gatccacttc gccgggctga
     8821 aagccgtcgg cgaatcggta caaaaaccgc tggaatatta cgacaacaat gtcaacggca
     8881 ctctgcgcct gattagcgcc atgcgcgccg ctaacgtcaa aaactttatt tttagctcct
     8941 ccgccaccgt ttatggcgat cagcccaaaa ttccatacgt tgaaagcttc ccgaccggca
     9001 caccacaaag cccttacggc aaaagcaagc tgatggtgga acagatcctc accgatctgc
     9061 aaaaagccca gccggactgg agcattgccc tgctgcgcta cttcaacccg gttggcgcgc
     9121 atccgtcggg cgatatgggc gaagatccgc aaggcattcc gaataacctg atgccataca
     9181 tcgcccaggt tgctgtaggc cgtcgcgact cgctggcgat ttttggtaac gattatccga
     9241 ccgaagatgg tactggcgta cgcgattaca tccacgtaat ggatctggcg gacggtcacg
     9301 tcgtggcgat ggaaaaactg gcgaacaagc caggcgtaca catctacaac ctcggcgctg
     9361 gcgtaggcaa cagcgtgctg gacgtggtta atgccttcag caaagcctgc ggcaaaccgg
     9421 ttaattatca ttttgcaccg cgtcgcgagg gcgaccttcc ggcctactgg gcggacgcca
     9481 gcaaagccga ccgtgaactg aactggcgcg taacgcgcac actcgatgaa atggcgcagg
     9541 acacctggca ctggcagtca cgccatccac agggatatcc cgattaagga acgaccatga
     9601 cgcaatttaa tcccgttgat catccacatc gccgctacaa cccgctcacc gggcaatgga
     9661 ttctggtttc accgcaccgc gctaagcgcc cctggcaggg ggcgcaggaa acgccagcca
     9721 aacaggtgtt acctgcgcac gatccagatt gcttcctctg cgcaggtaat gtgcgggtga
     9781 caggcgataa aaaccccgat tacaccagga cttacgtttt cactaatgac tttgcggcct
     9841 tgatgtctga cacgccagat gcgccagaaa gcaacgatcc tttgatgcgt tgccagagcg
     9901 cgcgcggtac cagccgggtg atctgctttt caccggatca cagtaaaacg ttgccagaac
     9961 tgagcgttgc ggcattgacg gaaatcgtca aaacctggca ggagcaaacc gcagagctgg
    10021 gaaaaacgta cccgtgggtg caggtctttg aaaacaaagg tgcggcgatg ggctgctcta
    10081 acccgcatcc gcacggacag atttgggcaa atagcttcct gcctaacgaa gctgagcgcg
    10141 aagaccgcct gcaaaaagaa tattttgccg aacagaaatc accaatgctg gtggattatg
    10201 ttcagcgcga gctggcagac ggtagccgta ccgttgtcga aaccgaacac tggttagccg
    10261 ttgtgcctta ctgggctgcc tggccgttcg aaacgctact gctgcccaaa gcccacgttt
    10321 tgcggatcac cgatttgacc gacgcccagc gcagcgattt ggcgctggcg ttgaaaaagc
    10381 tgaccagtcg ttatgacaac ctcttccagt gctccttccc ctactctatg ggctggcacg
    10441 gcgcaccatt taatggcgaa gagaatcaac actggcagct gcacgcgcac ttttatccgc
    10501 ctctgctgcg ctccgccacc gtacgtaaat ttatggttgg ttatgaaatg ctggcagaga
    10561 cccagcgaga cctgaccgca gaacaggcag cagagcgttt gcgcgcagtc agcgatatcc
    10621 attttcgcga atccggagtg taagaaatga gtctgaaaga aaaaacacaa tctctgtttg
    10681 ccaacgcatt tggctaccct gccactcaca ccattcaggc gcctggccgc gtgaatttga
    10741 ttggtgaaca caccgactac aacgacggtt tcgttctgcc ctgcgcgatt gattatcaaa
    10801 ccgtgatcag ctgtgcgcca cgcgatgacc gtaaagttcg cgtaatggca gccgattatg
    10861 aaaatcagct cgacgagttt tccctcaatg cgcccattgt cgcgcatgaa aactatcaat
    10921 gggcgaacta cgttcgtggc gtggtgaaac atctgcaact gcgtaacaac agcttcggcg
    10981 gtgtggacat ggtgatcagc ggcaatgtgc cgcagggtgc cgggttaagt tcttccgctt
    11041 cactggaagt cgcggtcgga accgtattgc agcagcttta ttatctgccg ctggacggcg
    11101 cacaaatcgc gcttaacggt caggaagcag aaaaccagtt tgttggctgt aactgcggga
    11161 tcatggatca gctaatttcc gcactcggca agaaagatca tgccttgctg attgactgtc
    11221 gctcactggg gaccaaagca gtttccatgc cgaaaggtgt ggctgtcgtc atcatcaaca
    11281 gtaacttcaa acgtaccctg gttggcagcg aatacaacac ccgtcgtgaa cagtgcgaaa
    11341 ccggtgcgcg tttcttccag cagccagccc tgcgcgatgt caccattgaa gagttcaacg
    11401 ctgttgcaca tgagctggac ccaatcgtgg cgaaacgcgt gcggcatatc ctgactgaaa
    11461 acgcccgcac cgttgaagct gccagcgcgc tggagcaggg cgacctgaaa cgtatgggcg
    11521 agttgatggc ggagtctcat gcctctatgc gcgatgattt cgaaatcacc gtgccgcaaa
    11581 ttgacactct ggtagaaatc gtcaaagctg tgattggcga caaaggtggc gtacgcatga
    11641 ccggcggcgg atttggcggc tgtatcgtcg cgttgatccc ggaagagctg gtgcctgccg
    11701 tacagcaagc tgtcgctgaa caatatgaag caaaaacagg tattaaagag actttttacg
    11761 tttgtaaacc atcacaagga gcaggacagt gctgaacgaa actcccgcac tggcacccga
    11821 tggtcagccg taccgactgt taactttgcg taacaacgca gggatggtag tcacgctgat
    11881 ggactggggt gcgactttac tttcagcccg tattccgctt tccgatggca gcgtccgcga
    11941 ggcgctgctc ggctgtgcca gcccggaatg ctatcaggat caggccgcgt ttctgggggc
    12001 ctctattggt cgttatgcca accgcatcgc caatagccgt tatacctttg acggtgaaac
    12061 cgtgaccctt tcgccaagtc agggcgttaa ccagctgcac ggcggcccgg aagggttcga
    12121 caaacgtcgc tggcagattg tgaaccagaa cgatcgtcag gtgctgtttg ccctgagttc
    12181 agatgatggc gatcagggct tcccgggtaa tctcggcgca acggtgcaat atcgtctgac
    12241 cgacgataac cgtatctcca ttacttatcg cgccacagtt gataaacctt gcccggtgaa
    12301 tatgactaat cacgtctatt tcaatcttga cggcgagcag tctgacgtgc gcaatcacaa
    12361 gttgcagatt ctggcggacg aatatcttcc ggttgatgaa ggcggcattc cgcacgacgg
    12421 cttgaaatct gtcgccggaa cgtcttttga tttccgcagc gccaaaatca tcgccagtga
    12481 gtttcttgcc gacgacgatc agcgcaaagt gaaaggttac gatcacgcat tcttgttaca
    12541 ggccaaaggc gatggcaaga aagtggcggc gcatgtctgg tcagcagatg aaaaattgca
    12601 gctgaaggtc tacaccaccg ctccggctct tcaattctac tccggcaact tcctcggcgg
    12661 cacaccgtcg cggggaaccg aaccttacgc cgactggcaa gggctggcgt tggaaagcga
    12721 gtttctgccg gacagcccga accaccctga atggccgcaa ccggactgct tcctgcgtcc
    12781 tggcgaagag tattccagcc tgacggaata tcagtttatt gctgagtaat gttaagccct
    12841 cgtcatgagg gctttatctt catattgttc aaatcaccag caaacaccga catatttgca
    12901 actcaatatt cacaacaacc ttacactgcg ccactatttt cgctatggtt atgcgtaagc
    12961 attgctgttg cttcgtcacg gcaatataat gagaattatt atcattaaaa gatgatttga
    13021 ggagtaagta tatggctgta actaagctgg ttctggttcg tcatggcgaa agtcagtgga
    13081 acaaagaaaa ccgtttcacc ggttggtacg acgtggatct gtctgagaaa ggcgtaagcg
    13141 aagcaaaagc agcaggtaag ctgctgaaag aggaaggtta cagctttgac tttgcttaca
    13201 cttctgtgct gaaacgcgct atccataccc tgtggaatgt gctggacgaa ctggatcagg
    13261 catggctgcc cgttgagaaa tcctggaaac tgaacgaacg tcactacggt gcgttgcagg
    13321 gtctgaacaa agcagaaacc gctgaaaagt atggcgacga gcaggtgaaa cagtggcgtc
    13381 gtggttttgc agtcactccg ccggaactga ctaaagatga tgagcgttat ccgggtcacg
    13441 atccacgtta cgcgaagctg agcgagaaag aactgccgct gacggaaagc ctggcgctga
    13501 ccattgaccg cgtgatccct tactggaatg aaactattct accgcgtatg aagagcggtg
    13561 agcgcgtgat catcgctgca cacggtaact ctttacgtgc gctggtgaaa tatcttgata
    13621 acatgagtga agaagagatt cttgagctga atatcccgac cggcgtgccg ctggtgtatg
    13681 agttcgacga gaatttcaaa ccgctgaaac gctattatct gggtaatgct gacgagatcg
    13741 cagcgaaagc agcggcggtt gctaaccagg gtaaagcgaa gtaaacgtca ttcgtttaaa
    13801 aatgagaaag ccgactgcaa gtgagccggc ttttttgttg ctaacaatgg agcacattgc
    13861 ctgatgcgac gctgcgcgtc ttatcaggcc tgtggtgatt catcggatac gccactctga
    13921 cggcgcatcc gacaattaaa ccttacccgc gacgcgcttt tactgcattc gccagttgac
    13981 gtaacagagc atcggtatct tcccagccga tgcaggcatc ggtgatgctc ttaccgtagg
    14041 ccagcggctc cccgctctcg aggctctgat tgccttccac cagatggctt tccaccatca
    14101 cgccaataat ggctgtttcg ccaccggcaa tctgctggca aacgtcagca cacacatcca
    14161 tctgcttttt gaattgtttg gacgagttag catggctgaa atcgatcatc acctgtgctg
    14221 gcaggcctgc tttgttcagc ccttctttca cttcagcaac gtgcttcgcg ctgtagttag
    14281 gctctttacc gccgcgcaga atgatatggc aatcaccgtt accgctggta ttcacaatcg
    14341 ccgaatgccc ccatttagtt acggacagga agcagtgcgg cgcaccggcg gcattaatgg
    14401 catcgatagc cactttaatc gtaccgtctg tgccattttt aaaacctacc ggacaagaaa
    14461 gaccagacgc cagttcgcgg tgcacctgcg attcggtggt acgtgcacca attgcgcccc
    14521 agctcatcag gtcagcgaga tattgtgggg tgatcatatc gagaaactca cccgccgctg
    14581 gcagaccgct gtcgttaata tcaagcagca atttacgggc tatacgcaga ccgtcgttga
    14641 tctggaagct gttatccata tgcggatcgt taatcagccc tttccagccc accgtggtac
    14701 gcggcttttc aaaatagacg cgcattacga tttccagctc atctttcagc tcttcacgca
    14761 gcgccagcaa gcgagtggca tactcttttg ctgcgacagg atcatgaatt gagcatgggc
    14821 caatcaccac caacaggcga tcatcattac ctttcaggat cttatggatc gcttttcggg
    14881 catgggcaac cgtattcgcg gcattttcag tagcggggaa tttttccagc aatgcgacag
    14941 gaggaagtaa ctctttgatt tctttgatgc gtaaatcgtc gttctgataa ttcatgtctg
    15001 ttccagtgtt gccatactta tcttagtgaa tgcaatactt ccaatctata tcttccgtca
    15061 gaatgtgtaa acggggtttt acactatgaa cggattaatc ctggaattag caagaaaaac
    15121 gccagattgt cgcgaaaaac gagatctctc ctacaatttc taactgtaac tcctttgttt
    15181 gttaattatt tcaagattct ctgctgcgtt tcataacctg gctgaaatct taaaccaatg
    15241 ccttatattc acctgcaaat gcactgttgg aagaggttat ccgacataac gaccataaca
    15301 ggagcatcct atgaaaatga caaaactggc cacattattt ctgactgcca ctctaagcct
    15361 tgccagcggt gccgcactgg ctgccgatag cggagcgcaa actaataacg gccaggcaaa
    15421 cgccgcagct gatgcgggcc aggtagcccc tgacgcccgt gaaaatgtcg cgccaaataa
    15481 cgtcgacaat aacggggtaa ataccggttc tggtggcaca atgctgcatt cggatggttc
    15541 ttcaatgaac aatgacggaa tgaccaaaga tgaagagcac aagaacacca tgtgcaaaga
    15601 tggtcgctgt ccggatatca ataaaaaggt acaaaccggt gatggcatca ataatgatgt
    15661 cgataccaaa accgacggta ccacgcagta accctgcaaa atagccaata atgggagagc
    15721 ggagtctctc ccttttaatt tttcacttct aaaagcagta tgatgtcata aagttaagtg
    15781 agaataatta agggatgact cgtcatggcg cactcacact cacacacgtc ctcacacctg
    15841 ccagaagata ataatgctcg tcgcttgttg tatgctttcg gcgtgactgc cgggtttatg
    15901 ctggtagaag tcgttggtgg ttttctttct ggttctctgg cattgctggc cgatgcgggt
    15961 catatgttga ccgatactgc cgccctgctt ttcgccctac tcgccgtgca attttcacgt
    16021 cgtcctccca ccattcgcca cactttcggt tggctaagac tgaccacgct cgcggcgttt
    16081 gtgaatgcca tagccctcgt ggtaattacc attttgattg tctgggaggc gatagaacgc
    16141 ttccgcacgc cgcgtccggt cgagggcggc atgatgatgg caattgccgt ggctgggttg
    16201 ctggcaaata tactttcttt ctggttactt catcacggca gcgaagagaa aaacctcaac
    16261 gtacgagcag cggcactgca tgtgctcggg gacctgctgg gttcggttgg ggctattatt
    16321 gccgcgttga ttattatctg gactggctgg acacctgctg accccattct ctcgatactg
    16381 gtgtcgcttc tggttctgcg tagcgcgtgg cgactgttga aagatagcgt gaatgaatta
    16441 cttgaaggtg caccggtatc gctggatatc gctgaactga agcgtcgtat gtgccgggaa
    16501 atcccggaag tccgcaatgt gcaccatgta catgtatgga tggtaggcga gaagccggtg
    16561 atgacgttgc atgtgcaggt gatcccgcca cacgatcacg atgccttgtt ggatcagatc
    16621 caacactacc tgatggatca ctatcagatt gagcacgcca ccattcagat ggaatatcaa
    16681 ccttgtcatg ggccagactg ccacctgaat gaaggggtgt ctggccattc acatcatcac
    16741 cattaatggg acagcgcgcg tgagcctctt tcacgtgcgc tgttgatcca catccggctg
    16801 ccgttgagcg caataaaggt caggatgatg tactccagag acatggcgta aacgccctga
    16861 agtgcaaaaa taacgacgct aatcacgtta ataatcaccc acaacagcca gttttccaca
    16921 tacttacgcg tcatcagaat cattgccacg atagataaca ccatcatgca tgaatcccag
    16981 aacgggaaag cgtccggttg cagttcaggc atcacaacct gtaatcctaa tgcttgcatg
    17041 atcatgactg ccaggcgggt caaaaatgca aacaccggat tgataaatac cgtcatcaga
    17101 ccaatcgaaa caacgcaaac cgccaaccag ctgagtgcct tcggcaatgg caaccagcga
    17161 attttcaact ccgcctcgtt ctgactggtt tgtcgcgacc acgcatacca accgtaaata
    17221 ttcgcggcaa agaaaaacac ctgtaatagc aggctggcat acagctgaat ctgaaagaaa
    17281 ataatgccaa acaaggtgac gttgatcagg ccaaagaagt agttgctgat cttctccaga
    17341 ctggcaaggc caatacacag caacccggcg atcgtgccta ccgcttcgat ccatgagaga
    17401 tcataaccgc ctgcccctat tggtatatgt accaggatat tctgcacact aaaaaaatcc
    17461 atctttcccc caaagcgtaa cgttacgcct tattattcgt tatccacgta gtgtagccgc
    17521 aaaatccagc atacgattga gcggcaccag cgccctctct cgcagccttt catcaacatg
    17581 aacctcgtga ttgcttcctt cctgttctaa tgcctctgcg atggactgaa ggccattcat
    17641 ggccatccac ggacaatgcg cgcagctgcg gcaggttgca ccctcacctg cggttggtgc
    17701 ttccagtaac tctttatctg gcactgcctg ctgcattttg tagaaaatgc cccgatcggt
    17761 tgccacgata agccgctgat gtggcaatgt tttcgcagca gcgatcagtt gactggtgga
    17821 accgaccgca tccgccatat cgacaatagc ttgtggtgat tctggatgca ccagtatggc
    17881 agcatccggg tattcttctt gcaagcgggt taacgcctga gtcttaaatt catcatgcac
    17941 aatacaggca ccctgccagc atagaatgtc tccacccgtc tgtttttgca cgtaacgccc
    18001 cagatgtttg tcgggtgccc agatgatttt ttcacccaaa ctatcaagat gatcaataag
    18061 ttcgacggca atgcttgaag ttaccaccca atctgcgcgc gcttttaccg cagcagaagt
    18121 gttggcgtag acgacgacag tacgatcggg atgggcatcg caaaatgcgt taaattcttc
    18181 aacagggcag ccgagatcca gtgaacattc agcctgaagt gtcggcatca gaattgtttt
    18241 ttccggactg agaattttgg cggtttctcc cataaatctc accccagcga ctaacaaagt
    18301 agaagcggga tgctttgcac cgaagcgcgc catttccaga gaatcagaaa tacagccacc
    18361 ggtttcttct gccagttgtt gaatttcggg atcggtatag tagtgggcaa ccatcaccgc
    18421 attacgttct tttagcagac gttttatctt ctcgcggtaa tacgcttttt catcaatgct
    18481 taacggcgtc ggcttcgggg ggaaaggata aatcgccgtg tctggatcaa acattacgct
    18541 catcttacca tctcgtttta caggcttaac gttaaaaccg acattagcgt aatattcgct
    18601 gttatcggtg taatttgttt tatatgctaa acaagatagt cgaatggtgg ggaaaagtca
    18661 ctcgaattac gcctaattcc accgacgtag agttgcttgc acagtaagct ctgacgatgt
    18721 catcaggttt acaaagtgaa attgaggatg cctg
//
LOCUS       DAZGLJ010000077        18656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000077 DAZGLJ010000000
VERSION     DAZGLJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.077"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(69..623)
                     /locus_tag="ACHK49_002369"
     CDS             complement(69..623)
                     /locus_tag="ACHK49_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO9263008.1"
                     /translation="MNKKLMYIFAIFIVAAITCISHPKKTTLRDKAMVNYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            724..1431
                     /locus_tag="ACHK49_002370"
     CDS             724..1431
                     /locus_tag="ACHK49_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO9263009.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSCVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            1428..2879
                     /gene="djlC"
                     /locus_tag="ACHK49_002371"
     CDS             1428..2879
                     /gene="djlC"
                     /locus_tag="ACHK49_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFO9263010.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(2939..4609)
                     /gene="hscC"
                     /locus_tag="ACHK49_002372"
     CDS             complement(2939..4609)
                     /gene="hscC"
                     /locus_tag="ACHK49_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO9263011.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELGALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQNSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(4693..5628)
                     /gene="rihA"
                     /locus_tag="ACHK49_002373"
     CDS             complement(4693..5628)
                     /gene="rihA"
                     /locus_tag="ACHK49_002373"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO9263012.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(5746..6471)
                     /gene="gltL"
                     /locus_tag="ACHK49_002374"
     CDS             complement(5746..6471)
                     /gene="gltL"
                     /locus_tag="ACHK49_002374"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO9263013.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(6471..7145)
                     /gene="gltK"
                     /locus_tag="ACHK49_002375"
     CDS             complement(6471..7145)
                     /gene="gltK"
                     /locus_tag="ACHK49_002375"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO9263014.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(7145..7885)
                     /gene="gltJ"
                     /locus_tag="ACHK49_002376"
     CDS             complement(7145..7885)
                     /gene="gltJ"
                     /locus_tag="ACHK49_002376"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO9263015.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            complement(8055..8963)
                     /gene="gltI"
                     /locus_tag="ACHK49_002377"
     CDS             complement(8055..8963)
                     /gene="gltI"
                     /locus_tag="ACHK49_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO9263016.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            9418..11295
                     /locus_tag="ACHK49_002378"
     CDS             9418..11295
                     /locus_tag="ACHK49_002378"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO9263017.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSFILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            complement(11373..12911)
                     /gene="lnt"
                     /locus_tag="ACHK49_002379"
     CDS             complement(11373..12911)
                     /gene="lnt"
                     /locus_tag="ACHK49_002379"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO9263018.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIVSLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGTGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLLGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(12936..13814)
                     /gene="corC"
                     /locus_tag="ACHK49_002380"
     CDS             complement(12936..13814)
                     /gene="corC"
                     /locus_tag="ACHK49_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO9263019.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(13904..14371)
                     /gene="ybeY"
                     /locus_tag="ACHK49_002381"
     CDS             complement(13904..14371)
                     /gene="ybeY"
                     /locus_tag="ACHK49_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO9263020.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(14368..15408)
                     /locus_tag="ACHK49_002382"
     CDS             complement(14368..15408)
                     /locus_tag="ACHK49_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO9263021.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(15561..16985)
                     /gene="miaB"
                     /locus_tag="ACHK49_002383"
     CDS             complement(15561..16985)
                     /gene="miaB"
                     /locus_tag="ACHK49_002383"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO9263022.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            17131..18306
                     /gene="ubiF"
                     /locus_tag="ACHK49_002384"
     CDS             17131..18306
                     /gene="ubiF"
                     /locus_tag="ACHK49_002384"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO9263023.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGGNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(18460..18534)
                     /locus_tag="ACHK49_002385"
     tRNA            complement(18460..18534)
                     /locus_tag="ACHK49_002385"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18500..18502),aa:Gln,seq:ctg)
BASE COUNT         4557 a         4649 c         4815 g         4635 t
ORIGIN      
        1 ttttctatat ttttatcttt gctggtttga tagggaaaat actccatctg ttcggttgac
       61 agtcgacgtt aatgtgcggg aagcccggca agtaatcttt ccgcattttt gtaatgttgt
      121 ttggcggcca gttttaacca tttttgagat tcataatggt taacattaat acctgagcca
      181 tttctgtaga tcaaacctaa agtatattga gcatgagcat acccttgttc agctgcttcc
      241 ttataccaga gaattgcttt acgggtgtcg ggtttgaccc ccagaccttt gaggtagatc
      301 caccccagtt tgctttgtgc gcgcggattt tcctgttctg ctgattgctc ataccagtat
      361 ctcgcctgtg tataatccag tggtttaccg tcgctaccat gaagataaaa ttctccaagg
      421 cgatattgcg acgttgagtg accatgaatc gcggaaagct ccgtggccgc cgtggtaaac
      481 ggtagactgc ccggtgagct taaataatca aaggcataat tcaccatggc tttatcacgc
      541 aacgttgttt tcttggggtg gctaatacag gtaattgctg caactataaa tattgcgaat
      601 atatacatta attttttatt catatgtgaa tgcttcgatt ttctttttat tttgaaagcg
      661 tcaagtttat actcatcaat aataaaaagt agtcagttaa tgcacttaag ggattattaa
      721 caaatgaata aggaagaaca atatcttctt tttgcgcttt ctgcgcccat ggaaatactt
      781 aatcagggtt gcaaacccgc gcatgactcg ccaaaaatgt ataccgggat aaaagaattc
      841 gatctctcat catcctgggg aataaataat cgtgatgatt taattcagac tatttatcag
      901 atgactgatg atggtcatgc caatgattta gctggcttat atctgacatg gcatcgctct
      961 tcccctgaag agtggaaagc attaattgct ggtggctcgg agagagggtt gatttatacc
     1021 caattcgttg cccaaaccgc tatgtgctgt ggagaagggg gaatcaaagc ctgggattat
     1081 gttcgaatgg ggtttctgtc acgggtcggt gtgctcaaca actggctgac ggaagaagag
     1141 agtttatggc tgcaatcgtg cgtctatgta cgagctcacc attactatca cagctggatg
     1201 cactattttt ccgcctattc gttaggacgt ctctactggc aatcttctca gtgcgaagac
     1261 aacacttcgc tacgcgaggc gctaaccctg tacaaatacg atagcgccgg gagtcggatg
     1321 ttcgaagagc tggcagcggg aagcgatcgt ttctatgcca cgcttccctg gcagccatta
     1381 actgtccagc ctgaatgccc ggtaacgctt aaggatgtga gcgacctatg aaaacatgtt
     1441 ggcaaatcct cgaaattgaa agcacgacgc aaatagacat tatccgccag gcttatcttg
     1501 ctcgcttacc gttatgtcat cccgaaaccg atccgcaggg gtttaaagca ttacgccagg
     1561 cctatgaaga ggcgctgcga ctggcggtaa atcctgtcga ggaagcagat gatgaagaaa
     1621 aagatgccgc cgctgaacat gaaatactgc gtgcattcag gacattactg gattcagaaa
     1681 gtgatcgttt tcagccctcc gcctggcaga aatttattca gcaattaaat acctggaaca
     1741 tggaggatgt cgatcaatta cgctggccgc tgtgtgcaat cgctatagaa gcgcgatatc
     1801 tttcattaaa ttgtgcttct ttgctggcag agcgtttgaa ctggcattcg tttaatgaca
     1861 gcgaaggaat ggatgaggaa gaaagggagg cttttcttga ggccattcag gctggtgatt
     1921 gtttcgattt ccttagcctt ctggaatatc ccgttgcgtt gcagaaccag actgttgagt
     1981 attacttcgc gctggaacgt tgctgccgtt accatcctga ctacgtcact gcgtttttgg
     2041 cgatggaagg tccgtggttc attcctgatg atgcaaagtt acatcgcaaa ctgttgcgct
     2101 ggtacagctc ggtgcaaaca ggtatggcgg aactcattcc tgtagccaaa cagtggcaaa
     2161 tggaagaacc agaaagcgaa gatgcccgat attacttgtg tgctcaaagg ttgtactgcg
     2221 gcgaggggga aagcctgctt gccgatctct gcgcgtaccg ggaaagttac ccatctacac
     2281 aagctgataa tttgttgttg cagtggagca agcgtcattg cccggattat ttcgcgttat
     2341 tagtgatggt tatcgaagcg cggagcatgg tagatgcgca aggtcaaccg ctgaaatatg
     2401 ttcctggtga gagcgcccgg acgcggctgt tatgggcgga aattttacat agcggaaaat
     2461 tatcgccgtt aggtcaatcg tttattgagt cgttattctt caaacgcaaa gcatgggcgt
     2521 ggtggaaatc gagagtcggt agcgagacag aagaagattc accgttactg gatttgtatc
     2581 gggtagcgga acaggtagta cttgaagcgt ttccgaaaca agagatgctg gcccgtctta
     2641 atacaaggct ggaaggcgga gatgctcatc cattagaggc cattatcacc cggatgctgt
     2701 tgacgaaagt aaaactcgag ccggaggatg aagatgtcga tgagccaaca cctgaaaacc
     2761 atgaagaaaa aaatgatgag ggtgaaaaac cacagagcat taccagcatt atcaaaatca
     2821 gtttaacggt gctggttata ggttatgctc tcggcaaaat cgcgatgttg tttagttgac
     2881 gatgtgtttt ttgcgccgat gccggatgca gcatttacgc cgcatccggc agccgtgatt
     2941 atgaggtctc aatggctaaa taaacgctcg cccgccgcct gacctcatcg acgcgggcca
     3001 tatcgtttga ctgcaacgcc gcatcaaaat cggtgatcac ccggccaatc tcttctcgct
     3061 cgtcacccag cgcccgcgcc cacaactctt ccagtttggc cttaaaggtg cggttgatga
     3121 gcatatcgcg cggataaatt ttcaatgcgg ataaccgcgt ccgactctct tcaatctgct
     3181 gcgcgctcaa tgttaccggg ctatgattaa tgactctgga cttaacgctg ccatcttcca
     3241 gaagcacgtc aacttcaagc aagccgttga tatcataact aaagcgaata tcaatcgact
     3301 gataagcccc ggttttcttc aacggcacat cgaaggattc aaccagaatg ttatttttaa
     3361 ctttgtggtt ttctccctga tagacgttaa ccgtaattga attctgttcc gggtgcatgg
     3421 tcgagtaagt ttctacacgc gacacgggta ctgtggtgtt tcgttcaata attggcgaga
     3481 aaatgccgga aacgccctgg cggttaactt caacgcccaa cgagtaaggg caaatatcag
     3541 tgaggatcac ctcttcaata tcttcactgc gtaagcggca ggcggcctgg attgctgcgc
     3601 ccagcgcgac aatggtgctc gggtcgtaac tttgatacgg taatttgcca aacagacgca
     3661 cggcgattcg ctgcaccagc ggcatttgtg acgcgccgcc aaccagcacc agactgtcga
     3721 tttgactcgg cttcagacgt gcatcgcgca acgcctgttc aatcggcact cgcaagcgat
     3781 tgagcagcgg caaccacaaa tcttccagtt cgttctcgta aaattcgcac tcccgcgttt
     3841 catcctggta ctgccagcga atgtgcaatg gcgattgatt gctacatttt gccgcttcca
     3901 cacaggcgta caaggctccc agttcactct cgttaagcgt ggtcctggcg acatccgcgc
     3961 gttttaaaac ctcatcgacc agcatatggg taaaatcttc gccaccaaga aagttgtcgc
     4021 cagcggaggc gtgaacttca atcaccggcg tggcgtactc aagcaccgta acgtcaaacg
     4081 tgccgccacc gagatcaaac accagcgaac gggtattttg ttgggtatgc aggccatacg
     4141 ccatcgcagc tgctgtgggt tcattaatta agcgtaccgc atttaacccg gctaactccg
     4201 ctgctaaacg ggtatgcttg cgttgttcat cgctgaaata agccggaacg gagatcacca
     4261 catctttaat cggacgttgc agaaattctt cggcatcttc ttttaatgag cgtaatacca
     4321 aagaggacag ttctggcgcg ttaaatgtgt cgctgcctaa ccgccagttg gtattactgc
     4381 ccattgcacg tttaaataac gctgccgttt tatccggatg cgaagtacgc cgtgatacag
     4441 ccggttttcc gactaaaata tgattatttt catccatgct aattatggat ggtgttaaat
     4501 attcaccgaa cttatttgga attaattgcg cggcaccgtc tttccagacg gcaattaaac
     4561 tattggtagt gccgagatcg ataccaatgg cgagttctgc attatccatt gcatttatat
     4621 cttgaaagaa aaagagataa acagattaag accacccagg taacgcgtgg tgatcttaat
     4681 caatgacgtg tgttaagcgt aaaatttcag acgatcagcc agtaaatcaa caaagccctg
     4741 acgatcaaca tcgaccatta cagtggcatt cggtttattg ccagtcagat aataataatc
     4801 aacaaccgtc ataccctggc tatatttccc ctgtgtttcc acgccaaccc agcgctcaac
     4861 agtggtaaac aactcgggtt tcaacagcca ggcgatggtg catgggtcat gcagcggtgc
     4921 accgacaaag ccccattttt cgtctttatg atattcgagg aagaaatcca gcagttcggc
     4981 aacaatggtt gaaacagggt tgccaatcgc gcggaaacgc tcggtgtctt caacgtggat
     5041 ttgtgctttg tgagtaacat ccagaccggc catcaccact gggatccctg actggaagac
     5101 aatttctgcc gcttccgggt caacgtaaat attaaattca gccgcaggcg tccagttacc
     5161 aagccccatt gcgccgccca taatcacgat acgggcaatt ttgctatgca gttccggatg
     5221 gctattgagc agcaaggcaa cgttagtttg cggtccggta gacacaatcg tgacaggttc
     5281 cgcactttcg cgcagcgttt tcgccatcag ctctaccgcc gtacagtttt gcggtgcgaa
     5341 cgtcggttcc ggtaatgccg ggccatccag accgctttcg ccgtgcacat tgtccgcgat
     5401 aatcaactca cgcattaacg gttttaccgc gccgcctgct accggaatat cggtgcgatt
     5461 aagcaaggtc agcatacgca gaacattgcg taaggttttt tctggtgtct ggtttccggc
     5521 ggaagacgta attgctttga catcaagctc tggtgaggcg agggcgagaa ctattgcgat
     5581 agcgtcgtca tgacctgggt cgcaatctaa cagaattggc agtgccattg ttgctccttg
     5641 ttgtgtgctt ctttgcgaca agggtaacgc caggatgtaa cagatacgag gggcgaaacg
     5701 ataaagcgtg agatggcgcg cacttgggta tgcgcgccag agtgattaat gcaggatttt
     5761 cgcgaggaag tctttcgcgc ggtccgattt cggatcatcg aagaaagcgt ctttcggcga
     5821 gtcttcgaca attttaccct cgtccataaa gatcacccga ttcgccactt tgcgggcaaa
     5881 gcccatttcg tgggtcacca ccatcatggt cattccttcg ttcgccagtt ccaccatcac
     5941 gtccagcact tcgttgatca tctccggatc cagcgccgat gtcggttcgt cgaacagcat
     6001 cgcaatagga tccatacaca acgcgcgagc gattgccaca cgctgctgct gaccgccgga
     6061 aagctgcgcc ggaaatttat tggcatgtgc ggaaagtccc acgcgctcca gcaatttcaa
     6121 tgctttttca cgagcgggcg ctttatcgcg tttaagcact ttcacctgcg ccagagtcag
     6181 gttttcaata atcgacaaat gggggaacaa ctcaaaatgc tggaacacca tcccgacgcg
     6241 ggaacgcagc tttgccagat cggttttctt gtcattaacc acgataccat cgacggtgat
     6301 ttcaccttgc tgcactggtt cgaggccgtt gacggtttta atcagcgttg atttgccgga
     6361 accagacggg ccgcaaacca ccaccacttc gccttttttc acttcggttg agcagtcggt
     6421 cagcacctga aagtgaccat accattttga aacatttttc agggtaatca ttatgctgtc
     6481 cttcttttca agtagctgac caacaacgac gcgctaagac taataacgaa ataaacaaat
     6541 ccggcaaata ggatcatctc aacctgcgta ccatcacgct caccaatggt tgaggcggta
     6601 cggaagaaat cggccagaga taacacatac accagtgagg tatcctggaa cagtacgatg
     6661 ccctgagtga gcagaagcgg caccatcgcg cggaacgcct gcggcagaat aatcagtttc
     6721 atcgactgcc agtgagtcat tcccaacgcc agcgcggcgc tcgactgacc acgagaaata
     6781 ctttgaatac cagcacggat aatctctgaa tagtaggccg cttcaaacat tgaaaacgcc
     6841 accatcgccg aaattaaacg gatatcattt tttggtgata atcccagcac gttttgcaga
     6901 aaacctggca cgatcaggta aaaccacagc aaaaccataa ctaaaggaat cgagcggaat
     6961 acgttaacgt aggctttggc aaaccacgct acgggcgcaa agctggacaa gcgcatcacc
     7021 gccagcatgg tgccccacaa aataccaatc acgactgccg tgacggtgat tttcagcgtg
     7081 atcaccagcc cgtcgagcag atatggcagg gaagggacaa tggaactcca gtcaaactcg
     7141 tacattattt gccccccatg ttgccaggca ggcgaacttt acgttcaacc agcgtcatta
     7201 ccagcatgat aaccgcgtta atcaacacat acgccagcgt aatagcggta aacgactccc
     7261 aggcgtgggc tgagtaatca agcaatttac ccgcctgcgc cgccatatcc accagaccaa
     7321 tagtcgaggc gatggcggag tttttcacca ggttcatcat ctcggaagtc atcggcggga
     7381 cgataacgcg ataagcatta ggcagcagta cgtatcgata agcctgcggt agcgtcaggc
     7441 ccatcgccag cgcggcattt ttttgcccac gcggcaacga ctgaatggcg gcgcgtacct
     7501 gttcgcaaac acgggcggcg gtaaacagcc ccaggcagag catggatgaa aggaaaaact
     7561 gaatattggg atccagctcg gccttaaacc acataccgat tttctctggc agcaactccg
     7621 ggatcaccag ataccaggta aagaattgca caatcaacgg cacgttgcgg aacagttcga
     7681 catacaacgt acccagaccg gagagaaaac ggtttggaac ggtacgtaaa atgccgaaaa
     7741 atgaaccgac gaggaaagcg ataatccagg cgcagatcga caaagcgatc gtcacctgaa
     7801 aaccactcca gatccaacca agataggtgg tgttgccgaa cggggcttgt tgtaaaaaaa
     7861 taccccagtt ccagtctata gacataaatc tactccagaa aaaagagggt agcagcgtta
     7921 actgctaccc tcgaagattg ttacccagcg tattgcggtt ttcaggcccg atggggaacg
     7981 accatcaggc gtatagtctg tccgtgctac gtaacaatcg agagggctgg aatttccgcc
     8041 cctggttctt gtaattagtt cagtgctttg tcattcggtt ctttgaacag tgctttcatt
     8101 tcgtctgaca gttcgaaatt catgttcagg tttttcggcg gaattggatt tttgaaccat
     8161 ttatcaaacc atttttccgc ttcaccggaa gtctgcacct gagcgatagt gtcatccatc
     8221 agctttttga actgcggatc atctttacgc aacatgcaac cgtaggcttc ctgagactgc
     8281 ggcttgccga cgatatccca gttgtctggt ttcttcgctt tcgcacgttc accggccagc
     8341 agggcatcat ccatcataaa ggcaacggca cgaccgcttt ccagggtacg gaaagagtca
     8401 ccgtgatctt tggcgctaat gatgcgcata ttcatttttt gctcttcatt cagtttgttg
     8461 agcaaaactt cagaagtagt gccggaagtg acgactacgg ctttgccttt caggtcggca
     8521 aaatctttga tatcgccgcc ctttttggtc agcaggcgcg taccgaccac gaaaatagta
     8581 tcagagaaag ccgcctgttt ttggcgttcg acgttgttgg tggtagaacc acattcaaaa
     8641 tcgaaagtgc cgttttgcag cagtggaata cggttttgtg aggtaatcgg aatcagtttt
     8701 acctgcaagt ccggtttgtt gagtttcttt ttcactgctt caacaatggc gttggagtaa
     8761 tcctgcgagt aacccaccac tttttgctga ttgtcgtaat aagagaaagg tactgaagat
     8821 tcacggtgac cgacgacaat cacgccgttt ttggcgattt tgtccagcgt actgcccgct
     8881 accggggcgg cgtcatctgc ctgtgccagt cctgcggaaa gcgccagggc gaggattgct
     8941 gtggcaggtt tacgtaattg catatccaac tcctttatct tctgcgttaa gaacgcatgg
     9001 atacccgttg tgagtgtttg tgttgttatc gtctgcaact ttattgtgca gtgttgtgcc
     9061 tgttaggtaa catttagttt ggctaaatgt aaagatattg ctgttttatt gtttgttttt
     9121 gcgagatgcg ccgcaccatt ccgaagcaaa attcttaaac cgcacctttt tggtgctacc
     9181 gctgatttgt ggtggtgcaa cctggttgcg tcgcggctga tttcatcgct tacacagtaa
     9241 ataaagcaac ttacatgcca aaaaatactt aggggcgaat taattacgat tgtaatttta
     9301 ggctgaaaaa gtcatatttc ggacagaatt atcttttcgg catgggacgt ttgccgggat
     9361 gataactgca cacgggaaaa atgcttttta cctgtctttt caaggaagaa acaccttatg
     9421 tctgcatctt cggttaagcc gttaaatgtt caattacccg caataaccct tatccttttt
     9481 gcactctgta ttgggatatt ttgttatctc gcacaatgga tgagttatga agaggtcgat
     9541 caatccgcac ttatccatct cggtgcaaac gttgcgcctc ttactttgtc gggtgaaccc
     9601 tggcgcttat tgagcagtat ctttctgcac agtagtgttt ctcatctgct gatgaatatg
     9661 tttgcattcc tggtcgtggg aggcgtggcg gaacagattc tggggaaatg gcgactcctg
     9721 attacctggt tattctccgg cgtctttggt gggctcatca gcgcctgtta tgcgttacgc
     9781 gaaagtgagc agatagtcat cagcgttggg gcatccgggg cgattctggg aatagctggt
     9841 gcagcgatag caacacagtt tgcttcaggt acgggcacat accataaaaa ccagcggcga
     9901 gtatttcctc tgttgggtat ggtggcgctg acactgttgt acggtgcccg gcaaacagga
     9961 atagataacg cttgccacat tggcggcctg attgcgggtg gcgcgttggg ttggctgagc
    10021 gcgcgtttat ctgggcaaaa cagactcgtt acggaaggcg ggattattgt tgcgggcagt
    10081 cttcttctga ccggggctat ctggcttgcg cagcagcaga tggatgagtc agttttacag
    10141 gtcaggcaaa gcctgcgtga agcgttttat ccgcaggaga ttgaacaaga gcgacgacaa
    10201 aaaaaacaac agttagcgga ggaacgcaac gccctcaggg aaacattatc cgctccggta
    10261 agtcgtgaac aggccagtgg tgatttactc gctgagattg ccgatatcca tgatatggcg
    10321 atcagtcggg atggtaatac gttgtatgcc gcaattgaaa acaccaacag cattgttgtt
    10381 ttcgacctcg gacaaaagaa aatcctgcat acctttacag cgcccatagc gaaagaaaag
    10441 tcagtcaaac attgtggtgg ctgtaaagat cagggcgtca gatcgctggc attaagcccg
    10501 gatgaaaagt tgatttatgc gacgtcattt gaagcgaatg cgttatcggt cattaacgtg
    10561 gcgacagggg agattattca gtcaattacc accggtgcac atcctgacag ttttatcctc
    10621 tcgcgtgatg gcacaaaagc ctgggtgatg aatcgcacca gtaatagtgt gtcagcgatt
    10681 gatctggtga cttatcagca tgtggcggat atcccgctgg agaaatacga cgggacgggg
    10741 acgagcggta aaccaggcgc ctgggttatg gcactttccc cggatgaaag aacattactg
    10801 gttccgggag caggcagagg taacatcgtg cggatcaata ccatcacgca tcagaaagaa
    10861 gactttcccg caggtgatgc gcgtggaacg atatcggcga tgcgttttcg acctgaaaac
    10921 ggcgaggtta tttttgccga tagtcagggg atttcacgta taagcgtagg ggatcaacaa
    10981 gccagcatta tgacgcaatg gtgtagcagg agcgtttatt ccgttgaggg tattagcccg
    11041 gacggtcaat atttagcgtt ggtgtcatat ggcctgcaag gttatgtcat cctgctcaat
    11101 attaatgccg ggcagattat tggcgtttat cctgccagct acgttaatca ccttcgtttt
    11161 tcagcggatg gtagaaaaat atttgtcatg gcgaagaacg ggttgatcca aatggacagg
    11221 acgcgctcgc ttgatccgca ggcaattatt cgtcatcccc aatatggcaa tgtggcttgt
    11281 atccctgaac cgtaagaaaa aggggcatga taaccatgcc ccaaagctca atgagaaagt
    11341 acgttttgct catgcaggat acatcaggca catcaacgtt tacgtgctcg cagactcatc
    11401 agcacagcgg caaaaccaaa cagcgccgtc agcacccaca gcggccagtt gccggtacgt
    11461 gcatacggtg tgagtccggt ggtcggtgtc acattagtgg tcaacacctc gcgggtgaac
    11521 tgcgggatca tcgcctgaat ctcaccctgc gggccaatca ccgccgtaat gccgttgttg
    11581 gtgctgcgca acagtgggcg cgccagctcc agcgcacgca ttcgcgccat ctggaagtgc
    11641 tgccatggac caatagactt accaaaccac gcatcgttgg agatagtcag cagatagtcg
    11701 gtatccgggc ggaagttatc gcgcacttgc tcgccaagaa tgatctcgta gcaaatggcc
    11761 gcagtaagct caataccatt taccgacagc ggcggctgga tatatggccc acggctgaac
    11821 gacgacatcg gcagatcaaa gaacggtgct aacggacgca gaatcgactc cagcgggaca
    11881 aactcgccaa acggcaccag atggtttttg ttatagcgat cggctgattc gtagctgtac
    11941 ggtgcacctt tacccagcgt gatgatggtg ttgtaggtat cgtagcggtt ctgcttattg
    12001 agacgcgcgt cgacaatccc ggttaccagc gagctacctt tatcacgcaa ctcaccgtcc
    12061 agtgctttga ggaacggttg ctgattaatt tccagatcgg ttatcgccga ctccggccag
    12121 ataatcaacg atgatttgcc cagcagcggt gccgttgcgt tgtagtaaat cttcagcgta
    12181 ttaagaagct ggtctccgtc ccatttcagt gattgcggaa tatcgccctg aaccatcgaa
    12241 acctgaatgg ttttctccgg ttgcggggta aaccattgga tgtaacgcag cgggaaggga
    12301 agggcaaaca gcacgacggc caccaccagc ggacgccagt tgcgtttgac taacgccagt
    12361 gccagcaggc cgctaaccat catcagcagg aagttaatgg cttccacgcc cattattggt
    12421 gccagccctt ttaacgggcc atcaatctgg ctatagccga actgtaacca cgggaagcca
    12481 gtcagcaccc aaccgcgcag aaactcggtc acttgccaga gagcaggggc ggcaatcgcc
    12541 acgcgcagcc aggtggtttt cggccacagg cgcgacagca cgccagcaaa cagtccggta
    12601 tacagcgaca aatacgccgc cagcagcacc accaggaaga tgttaaccgg gccaggcatc
    12661 ccgccaaagg tcgcgatgct gacatagacc cagttaatac cggtgccaaa gaggccaaat
    12721 ccccagcaga agccaatagc ggcagactgg agcgggcggc ggttaaaggt cagcgcctga
    12781 agccccatca gcgaaacaat cgccgcaggc cagacatcgt aaggagagaa ggccagcgtt
    12841 ccgcaggcac cgaataataa cgccagcagc aggcgaatgc gctggcgttc aattaatgag
    12901 gcaaaagcca tgtagttatc tatccagttt cggttttatt catccagctt tggctggggt
    12961 gagtcatccg ggattttgac atgaacctga ataatacgcc gactatcggc catcgccact
    13021 ttgaactggt aaccgtcgat gtcgatagtt tcgccacgcg ccggaagatg cccaaacgcc
    13081 tgcatcacca gaccaccgat agtgtcgact tcttcatcgc taaagtgggt gccgaacgct
    13141 tcgttgaagt cttcaatgga agccagtgcg cgcacggtcc aggtatgacg actcagctga
    13201 cggaagtcga tatcatcttc ttcgtcatac tcgtcttcaa tctcaccaac aatcagttcc
    13261 aggatgtctt caatggtcac cagaccggaa accccaccga attcgtcaat aacgatcgcc
    13321 atgtggtaac gctgagagcg aaactctttc agcatccggt ctacgcgctt actttcagga
    13381 acgacaaccg cctgacgtaa cactttgtcc atgctgaagg cttcagcatc gctgcgcata
    13441 aacggtagca agtctttcgc catcagaatc ccttcaatgt gatctttgtc ttcgctaatc
    13501 accgggaaac gtgagtgggc ggactcgatg atgacatcaa ggcattcgtc cagcgtctgg
    13561 ttgcgtttca gggtaatcat ctgggagcgg gggatcatga tgtcgcgaac gcgttggtct
    13621 gcgatgtcca tcaccccttc gagcatatcg cgcgtatctt cgtcgataag gtcgttctgc
    13681 ccggaatcac ggatcagcgc cagcagttcg tcacggtttt tcggttcacc gtggaaaagt
    13741 tggctgagta acagggagaa aaatcccttc ttgttgctta tcgtgtcact actgtgtgaa
    13801 ttgtcgtcgc tcatggcgtc gtatgggttc tcatgttagt taatcaaaac gccgtcgtta
    13861 atcaccaacg gcggggacgt ctgccagtca aatgcctggc aaattattct ttctcggcaa
    13921 tgtacggatc ctcatagccc agagcaagca taatctctgt ttcgagggct tccatttctt
    13981 ctgcttcgtc atcttcgatg tgatcgtaac ctaacaaatg cagactgccg tgcaccacca
    14041 tatgcgccca gtgcgcctcc agtggtttgc cttgctcctg agcttccttc tcaaccacct
    14101 gacggcagat aaccagatcg cccagtagcg acatttccat gccaggcggc acttcaaacg
    14161 ggaaggagag cacgttggtc ggcttatcct taccgcgata ggtcagattc agactgtggc
    14221 tttcggcggt atcgaccacg cgaatagtca cttccgattc ttcctgaaac tgcgggatca
    14281 ccgcattcag ccatgtttga aactggctct cttccggtaa cccggaatta tcttcacatg
    14341 ccagttgtaa atcgaggatc acctgactca tttttgttcc tgttcttcgc gcttgcgttc
    14401 tgccgccagc gccgcttttc gtttttgttc ggcttcttcc caggcttcat aggcgttaac
    14461 gatacgcgcc accaccgggt gacgaaccac gtcttcgctg tggaagaagt taaagctgat
    14521 ctcttcgaca tcggccagca cttcgatggc gtgacgtaag cctgatttag tattacgcgg
    14581 caggtcgatc tgtgtgacgt cgccggtgat aaccgctttt gagttaaaac cgatacgggt
    14641 caggaacatc ttcatctgtt cgatggtggt gttctggctc tcatcgagaa tgataaacgc
    14701 gtcgttcagc gtacgaccac gcatataggc cagcggcgcg acttcaataa cgttgcgctc
    14761 aatcagtttc tcaactttct caaagcccag catttcaaac agcgcgtcgt acagcgggcg
    14821 cagatacggg tctactttct ggcttaaatc gccaggcagg aagcccagtt tctcaccggc
    14881 ttcgaccgcc ggacgagtca gcagaatacg gcgaatctcc tgacgctcca gggcatcaac
    14941 tgccgcagcc actgccaggt aggttttacc cgtacccgcc gggccaacgc cgaaggtaat
    15001 gtcatggtcg agaatattgg cgatgtactg cgcctggttt ggcgtgcgcg gcttaattac
    15061 gccgcgtttg gttttgatat tgaccgcttt gccgtactcc ggcacgctct ccgcactttg
    15121 ctccagtact cgcgcttctt taatcgcaag gtggatctgt tccggttcga tatcctgaat
    15181 ctgaccgcgc atcggggctg tatcgacata caggctacgc agaatgtctg ccgcagcggt
    15241 gacgcaaatc ggacggccgg tcagtttaaa gtggttgtcg cggcgattga tctcgatgcc
    15301 gagacggcgt tcgagctgct tgatgttgtc atcaaacggg ccgcacaggc tcaacagacg
    15361 cgcgttgtct gctggctcca gggtgatttc gcgagtgtct atgttcaaac cgttcctctt
    15421 atctgtatgt cgccggaagc tgaacattca ccggcctata aggaaattat tcacgccaca
    15481 ggaaaaaggc gcaagcattg cgataaagat ggggataaag agagaaaaaa caaggcccac
    15541 cggaacggcg ggcctgagaa ttacggctga taataaccca cgccaaggtc gttttctttg
    15601 cgggtacggg caatcaccga ttccggtgtt tctgccacgc gcagacccat ttcatcttca
    15661 gtacgcacca ctttaccgcg cagagagttc gggtagacgt cggtaatttc tacatcgacg
    15721 aatttaccga tcatatccgg tgtgccctcg aagttgacca cgcggttatt ttcggtacgc
    15781 ccggaaagct ccatgatgct cttacgcgaa gtcccttcta ccagaatacg ctgggtggtg
    15841 ccgagcatcc ggcggctcca tgccatcgct tgctgattga tacgttcttg cagaatatac
    15901 agacgttgct tcttctcttc ttccggaaca tcatcaacca tatcggcggc tggtgtaccc
    15961 ggacgtgcag agaagataaa gctgtagctc atgtcgaaat tgacgtcggc aatcagcttc
    16021 atcgttttct cgaagtcttc ggtggtttcg ccagggaagc caacaatgaa gtcagaactg
    16081 atctgaatat ccggacgcgc cgcacgcagt ttacggatga tcgctttgta ctccagtgcc
    16141 gtatgagtac ggcccatcag gttcagaatg cgatcggaac cgctctgtac cggcagatgc
    16201 aggaagctca ccagctccgg cgtgtcgcga tacacttcga tgatatcgtc ggtgaattcg
    16261 atcggatggc tggtggtaaa gcgaatacga tcgatcccgt cgatcgcagc aaccagacgc
    16321 aacagatcgg caaacgatcc ggtggtgccg tcgtagttct caccacgcca ggcattcaca
    16381 ttctgaccga gcaggttgac ttcacgcacg ccctgagccg caagctgggc gatctcaaac
    16441 agaatatcgt cggacggacg gcttacctct tcaccacggg tgtaaggcac cacgcagtag
    16501 gtgcaatatt tattgcagcc ttccatgatg gagacaaacg cggtcggccc ttcggcgcgc
    16561 ggttccggca gacggtcaaa cttctcgatt tccgggaagc tgatatctac aaccgggctg
    16621 cggtcgccgc gcacggagtt gatcatctcc ggcagacggt gcagcgtttg cggcccaaaa
    16681 ataatatcga catagtgggc gcgctggcga atgtgctcgc cttcttgcga tgccacgcag
    16741 ccaccgacgc cgataatcag gtctggattc ttctctttta acagtttcca gcgacccaac
    16801 tgatggaaga ctttttcctg agccttctcg cggattgagc aggtgttcag cagcagcaca
    16861 tccgcttctt ccgccacgtc ggtcagttga tagccgtggg tggcatccag cagatcggcc
    16921 atcttcgatg aatcgtactc gttcatctga cagccccagg ttttaatatg gagttttttg
    16981 gtcatcgact tgctcttgcg aaatagtagc caggaatgca gggcgcatag tgtaatgctt
    17041 tgctgccgtt gtgaccagta tgagcgttat cagcccttgg gggtaaaaat cctgtaaact
    17101 taaagcatat tgctaacagg atgattgacc atgacaaatc aaccaacgga aattgccatt
    17161 gtcggcggag gaatggtcgg cggcgcactg gcgctggggc tggcacagca cggattttcg
    17221 gtaacggtga tcgagcacgc agaacctgca ccgtttgtcg ctgatagcca accggacgtg
    17281 cggatctcgg cgatcagcgc cgcatcggta tcattgctta aagggttagg ggtctgggat
    17341 gcagtacagg ctatgcgttg ccatccttac cgcagactgg aaacgtggga gtgggaaacg
    17401 gcgcatgtga tgtttgacgc cgctgaactt aagctgccgc tgcttggcta tatggtggaa
    17461 aacaccgtcc tgcaacaggc attgtggcag gcgctggaag cgcatccgaa agtaacgtta
    17521 cgtgtgccag gctcgctgat tgcattacat cgagataatg atcttcagga gctggaactg
    17581 aagggcggtg aagtgattcg cgcgaagctg gtgattggtg ctgacggcgc aaattcgcag
    17641 gtgcggcaga tggcgggaat tggcgttcat gcctggcagt atgcgcagtc gtgtatgttg
    17701 attagcgtac agtgtgagaa cgatcccggc gacagcacct ggcagcaatt taccccggac
    17761 ggaccgcgtg cgtttctgcc gttgtttgat aactgggcat cgctggtgtg gtatgactct
    17821 ccggtacgta ttcgccagtt gcagaatatg aatatggcgc aactccaggc ggaaatcgcg
    17881 aagcatttcc cgtcgcgtct gggttacgtt acaccgcttg ccgctggtgc gtttccgctg
    17941 acgcgccgac atgcgttgca gtatgtgcag ccggggcttg cgctggtggg cgatgccgcg
    18001 cataccatcc atccgctggc ggggcagggg gggaatcttg gttatcgtga tgtcgatgcc
    18061 ctgattgatg ttctggtgaa tgcccgcagc tacggtgaag cgtgggccag ttatcctgtc
    18121 ctcaagcgtt accagatgcg gcgcatggcg gataacttca ttatgcaaag tggtatggat
    18181 ctgttttatg ccggattcag taataatctg ccgccgctgc gttttgtgcg taatctcggg
    18241 ttaatggcgg cagagcgtgc tggcgtgttg aaacgtcagg cgctgaaata tgcgttaggg
    18301 ttgtagcctt acaacattgc cggatgcgtg ccaaccgtag gtcggataag acgcggcagc
    18361 gtcgcatccg acattgaagg ataagacgtg tcaacatcgc attcgacatt gaatgaacgc
    18421 agaaaagcaa aaagctcgcc gaagcgagct tttttaatgt ggctggggta cgaggattcg
    18481 aacctcggaa tgccggaatc agaatccggt gccttaccgc ttggcgatac cccaacaaat
    18541 tggtttttga atttgccgaa cgtattcgac acattcagaa tttggtggct acgacgggat
    18601 tcgaacctgt gaccccatca ttatgagtga tgtgctctaa ccaactgagc tacgta
//
LOCUS       DAZGLJ010000078        18566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000078 DAZGLJ010000000
VERSION     DAZGLJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.078"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..853
                     /locus_tag="ACHK49_002386"
     CDS             <1..853
                     /locus_tag="ACHK49_002386"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFO9263024.1"
                     /translation="ASYALGTTAANARALDSTEERIQDYRREQRILSDNSISGQSVQR
                     GTLPPDTRPYLAPDWPTLAENTMPSVIALSAIEQPYGFAENSPAWLIPGVQQNAATAA
                     LTHYRAWQKGWAKQDAKRQHQTICGGFVIEGNRVLTAAHCVDRQEALRIRTAGGEWRA
                     AHVVGADVTQDVALLEFEGDPLPPIKVANTLPRPGQDVMAIGSPSGYGFAVGVGIVAW
                     HGLDAKMLSPNDFMLVTASVVGGNSGGVVVNTHGEAVSLVSYGSGSYTQTVPIDRALT
                     VAGQLAK"
     gene            complement(1288..1992)
                     /locus_tag="ACHK49_002387"
     CDS             complement(1288..1992)
                     /locus_tag="ACHK49_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFO9263025.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     gene            complement(2004..3062)
                     /gene="intI1"
                     /locus_tag="ACHK49_002388"
     CDS             complement(2004..3062)
                     /gene="intI1"
                     /locus_tag="ACHK49_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFO9263026.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SEALLQIVGGDKLIIPFC"
     gene            3219..3692
                     /gene="dfrA1"
                     /locus_tag="ACHK49_002389"
     CDS             3219..3692
                     /gene="dfrA1"
                     /locus_tag="ACHK49_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFO9263027.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            3785..4623
                     /gene="aadA1"
                     /locus_tag="ACHK49_002390"
                     /pseudo
     CDS             3785..4623
                     /gene="aadA1"
                     /locus_tag="ACHK49_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            4740..5087
                     /locus_tag="ACHK49_002391"
     CDS             4740..5087
                     /locus_tag="ACHK49_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFO9263028.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            5081..5920
                     /gene="sul1"
                     /locus_tag="ACHK49_002392"
     CDS             5081..5920
                     /gene="sul1"
                     /locus_tag="ACHK49_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFO9263029.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            6048..6548
                     /locus_tag="ACHK49_002393"
     CDS             6048..6548
                     /locus_tag="ACHK49_002393"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9263030.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     gene            complement(6724..7509)
                     /gene="istB"
                     /locus_tag="ACHK49_002394"
     CDS             complement(6724..7509)
                     /gene="istB"
                     /locus_tag="ACHK49_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS1326 family helper ATPase
                     IstB"
                     /protein_id="HFO9263031.1"
                     /translation="MMQHEGHVRILKSLKLFGMAHAIEELGNQNSPAFNQALPMLDSL
                     IKAEVAEREVRSVNYQLRVAKFPVYRDLVGFDFSQSLVNEATVKQLHRCDFMEQAQNV
                     VLIGGPGTGKTHLATAIGTQAVMHLNRRVRFFSTVDLVNALEQEKSSGRQGQIANRLL
                     YADLVILDELGYLPFSQTGGALLFHLLSKLYEKTSVILTTNLSFSEWSRVFGDEKMTT
                     ALLDRLTHHCHILETGNESYRFKHSSTQNKQEEKQTRKLKIET"
     gene            complement(7496..9019)
                     /gene="istA"
                     /locus_tag="ACHK49_002395"
     CDS             complement(7496..9019)
                     /gene="istA"
                     /locus_tag="ACHK49_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS1326 family transposase"
                     /protein_id="HFO9263032.1"
                     /translation="MINVAILSAIRRWHFRDGASIREIARRSGLSRNTVRKYLQSKVV
                     EPQYPARDSVGKLSPFEPKLRQWLSTEHKKTKKLRRNLRSMYRDLVALGFTGSYDRVC
                     AFARQWKDSEQFKAQTSGKGCFIPLRFACGEAFQFDWSEDFARIAGKQVKLQIAQFKL
                     AHSRAFVLRAYYQQKHEMLFDAHWHAFQIFGGIPKRGIYDNMKTAVDSVGRGKERRVN
                     QRFTAMVSHYLFDAQFCNPASGWEKGQIEKNVQDSRQRLWQGAPDFQSLADLNVWLEH
                     RCKALWSELRHPELDQTVQEAFADEQGELMALPNAFDAFVEQTKRVTSTCLVHHEGNR
                     YSVPASYANRAISLRIYADKLVMAAEGQHIAEHPRLFGSGHARRGHTQYDWHHYLSVL
                     QKKPGALRNGAPFAELPPAFKKLQSILLQRPGGDRDMVEILALVLHHDEGAVLSAVEL
                     ALECGKPSKEHVLNLLGRLTEEPPPKPIPIPKGLRLTLEPQANVNRYDSLRRAHDAA"
     gene            9142..10686
                     /locus_tag="ACHK49_002396"
     CDS             9142..10686
                     /locus_tag="ACHK49_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO9263033.1"
                     /translation="MYSYEDRLRAVRLYLKLGRRMSATLRQLGYPTKNSLKAWLAEFE
                     RNQDLRRGYQRIKRQYTDEQKQRAVDHYIEQGYCLSHTIRSLGYPSREALRAWIRDLR
                     PEFARTVVGSSAPTVARSRLEKQQAVIALNLRVGSAKDVADTVGVSRPTLYNWQHRLL
                     GKVPLKPMTKKKGDTSLEQRHEALLRELAELESQNQRLRMENAILEKASELIKKDMGI
                     NPLELTSREKTKVVDALRVTFPLANLLCGLKLARSTYFYQRLRQTRPDKYTQVREVIR
                     TIFEDNYRCYGYRRIDSALRLGGMRVSEKVVRRLMAQERLVVRTPRRRRFSAYAGDPT
                     PAVPNLLNRDFHASAPNTKWLTDLTEIHIPAGKVYVSPIVDCFDGLVVAWNIGTSPDA
                     NLVNTMLDHAVRTLRPGEHPVIHSDRGSHYRWPAWIRRTENAQLTRSMSKKGCSPDNA
                     ACEGFFGRLKTELIYPRNWQHVTLKDLMTRIDAYIHWYNERRIKVSLGGRSPIEYRHA
                     VGLMSV"
     gene            complement(10737..11597)
                     /locus_tag="ACHK49_002397"
     CDS             complement(10737..11597)
                     /locus_tag="ACHK49_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HFO9263034.1"
                     /translation="MDEYPIIDLSHLLPAAQGLARLPADERIQRLRADRWIGYPRAVE
                     ALNRLEALYAWPNKQRMPNLLLVGPTNNGKSMIVEKFRRTHPASSDADQEHIPVLVVQ
                     MPSEPSVIRFYVALLAAMGAPLRPRPRLPEMEQLALALLRKVGVRMLVIDELHNVLAG
                     NSVNRREFLNLLRFLGNELRIPLVGVGTRDAYLAIRSDDQLENRFEPMMLPVWEANDD
                     CCSLLASFAASLPLRRPSPIATLDMARYLLTRSEGTIGELAHLLMAAAIVAVESGEEA
                     INHRTLSMAC"
     gene            complement(11600..13279)
                     /locus_tag="ACHK49_002398"
     CDS             complement(11600..13279)
                     /locus_tag="ACHK49_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO9263035.1"
                     /translation="MATDTPRIPEQGVATLPDEAWERARRRAEIISPLAQSETVGHEA
                     ADMAAQALGLSRRQVYVLIRRARQGSGLVTDLVPGQSGGGKGKGRLPEPVERVIHELL
                     QKRFLTKQKRSLAAFHREVTQVCKAQKLRVPARNTVALRIASLDPRKVIRRREGQDAA
                     RDLQGVGGEPPAVTAPLEQVQIDHTVIDLIVVDDRDRQPIGRPYLTLAIDVFTRCVLG
                     MVVTLEAPSAVSVGLCLVHVACDKRPWLEGLNVEMDWQMSGKPLLLYLDNAAEFKSEA
                     LRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDYDSE
                     NKAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAVARVGVPAVVTRATSFLV
                     DFLPILRRTLTRTGFVIDHIHYYADALKPWIARRERWPSFLIRRDPRDISRIWVLEPE
                     GQHYLEIPYRTLSHPAVTLWEQRQALAKLRQQGREQVDESALFRMIGQMREIVTSAQK
                     ATRKARRDADRRQHLKTSARPDKPVPPDTDIADPQADNLPPAKPFDQIEEW"
     gene            complement(13414..14061)
                     /locus_tag="ACHK49_002399"
                     /pseudo
     CDS             complement(13414..14061)
                     /locus_tag="ACHK49_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003132006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(14058..14294)
                     /gene="merE"
                     /locus_tag="ACHK49_002400"
     CDS             complement(14058..14294)
                     /gene="merE"
                     /locus_tag="ACHK49_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum mercury transporter MerE"
                     /protein_id="HFO9263036.1"
                     /translation="MNAPDKLPPETRQPVSGYLWGALAVLTCPCHLPILAAVLAGTTA
                     GAFLGEHWGVAALALTGLFVLAVTRLLRAFRGGS"
     gene            complement(14291..14653)
                     /gene="merD"
                     /locus_tag="ACHK49_002401"
     CDS             complement(14291..14653)
                     /gene="merD"
                     /locus_tag="ACHK49_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance co-regulator MerD"
                     /protein_id="HFO9263037.1"
                     /translation="MSAYTVSQLAHNAGVSVHIVRDYLVRGLLRPVACTTGGYGVFDD
                     AALQRLCFVRAAFEAGIGLDALARLCRALDAADGAQAAAQLAVLRQLVERRRAALAHL
                     DAQLASMPAERAHEEALP"
     gene            complement(14671..16365)
                     /gene="merA"
                     /locus_tag="ACHK49_002402"
     CDS             complement(14671..16365)
                     /gene="merA"
                     /locus_tag="ACHK49_002402"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA];
                     GO_process: GO:0050787 - detoxification of mercury ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HFO9263038.1"
                     /translation="MSTLKITGMTCDSCAVHVKDALEKVPGVQSADVSYAKGSAKLAI
                     EVGTSPDALTAAVAGLGYRATLADAPSVSTPGGLLDKMRDLLGRNDKTGSSGALHIAV
                     IGSGGAAMAAALKAVEQGARVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRESP
                     FDGGIAATTPTIQRTALLAQQQARVDELRHAKYEGILEGNPAITVLHGSARFKDNRNL
                     IVQLNDGGERVVAFDRCLIATGASPAVPPIPGLKDTPYWTSTEALVSETIPKRLAVIG
                     SSVVALELAQAFARLGAKVTILARSTLFFREDPAIGEAVTAAFRMEGIEVREHTQASQ
                     VAYINGEGDGEFVLTTAHGELRADKLLVATGRAPNTRKLALDATGVTLTPQGAIVIDP
                     GMRTSVEHIYAAGDCTDQPQFVYVAAAAGTRAAINMTGGDAALNLTAMPAVVFTDPQV
                     ATVGYSEAEAHHDGIKTDSRTLTLDNVPRALANFDTRGFIKLVVEEGSGRLIGVQAVA
                     PEAGELIQTAALAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFNKDVKQLSCCAG"
     gene            complement(16417..16839)
                     /gene="merC"
                     /locus_tag="ACHK49_002403"
     CDS             complement(16417..16839)
                     /gene="merC"
                     /locus_tag="ACHK49_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial transporter MerC"
                     /protein_id="HFO9263039.1"
                     /translation="MGLMTRIADKTGALGSVVSAMGCAACFPALASFGAAIGLGFLSQ
                     YEGLFISRLLPLFAALAFLANALGWFSHRQWLRSLLGMIGPAIVFAATVWLLGNWWTA
                     NLMYVGLALMIGVSIWDFVSPAHRRCGPDGCELPAKRL"
     gene            complement(16875..17150)
                     /gene="merP"
                     /locus_tag="ACHK49_002404"
     CDS             complement(16875..17150)
                     /gene="merP"
                     /locus_tag="ACHK49_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HFO9263040.1"
                     /translation="MKKLFASLALAAAVAPVWAATQTVTLAVPGMTCAACPITVKKAL
                     SKVEGVSKVDVGFEKREAVVTFDDTKASVQKLTKATADAGYPSSVKQ"
     gene            complement(17164..17514)
                     /locus_tag="ACHK49_002405"
     CDS             complement(17164..17514)
                     /locus_tag="ACHK49_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transport protein MerT"
                     /protein_id="HFO9263041.1"
                     /translation="MSEPQNGRGALFAGGLAAILASTCCLGPLVLVALGFSGAWIGNL
                     TVLEPYRPLFIGAALVALFFAWKRIYRPVQACKPGEVCAIPQVRATYKLIFWIVAVLV
                     LVALGFPYVVPFFY"
     gene            17586..17771
                     /locus_tag="ACHK49_002406"
                     /pseudo
     CDS             17586..17771
                     /locus_tag="ACHK49_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            complement(17748..18445)
                     /locus_tag="ACHK49_002407"
     CDS             complement(join(17748..18196,18196..18445))
                     /locus_tag="ACHK49_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="HFO9263042.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
BASE COUNT         3886 a         5388 c         5435 g         3857 t
ORIGIN      
        1 ggccagttat gcgttgggta cgaccgctgc aaatgctcgt gccctggata gcacagaaga
       61 acgaatacaa gactatcggc gtgagcagcg catattgtcc gacaattcaa tcagcggcca
      121 gagcgtccag cgcggcacac tgccacccga taccagaccc taccttgcac cggattggcc
      181 aacactggct gaaaatacaa tgccgtctgt tattgctttg tcagcaatcg agcagcccta
      241 cggctttgct gaaaacagtc cggcctggct tattcctggc gttcaacaaa acgctgccac
      301 agcagcattg acgcactacc gcgcctggca aaaaggctgg gcaaaacaag acgcaaagcg
      361 acagcatcaa actatatgcg gcggtttcgt catcgaaggc aaccgagtgc taacagcggc
      421 gcattgtgtg gacaggcaag aagccttgcg aatccgtaca gcgggcggtg aatggcgggc
      481 ggctcatgtt gtcggtgctg atgtaacgca ggacgttgct ttgcttgagt ttgaaggcga
      541 cccattgcca ccgattaagg tggccaacac tctacccagg ccgggccagg acgttatggc
      601 cataggctcg ccgtccggtt acggctttgc tgtcggtgtc ggcattgttg cctggcacgg
      661 cctcgatgca aagatgctat cccctaatga tttcatgctt gtaacggctt cagtcgtggg
      721 cggtaattcc ggcggcgtgg tcgtgaatac gcacggtgaa gcggtttcat tggttagcta
      781 tggcagcggc agttatacgc aaacagtgcc gattgaccga gctttaaccg ttgctggcca
      841 gctggccaag taacgctacc ccaaggccgg gcaaattttg cccaggtcaa ttaccaagcg
      901 ccgaagggcg gcgcatgaag aaaaaaaaca ttaaaaaact ggagtaaatc actatgaaaa
      961 ttcgcaatgt attttgttca ctggttgttg cctttgctgt tgtgtctgtt tcacaggcag
     1021 cgactgccac tgatttggac gttgtgcata ctgcgtatga agcctacgaa ggagctgcaa
     1081 aggctttaga gcgcagcatt gacgttgtgc atagcaaact gaacaccgca ggtttaacgc
     1141 cagaagaaat gcaaaagctg caactacagc ttgatgcttt gaaggcacag catgacgtgg
     1201 cactgggccg ggtgatggtt gaagctgcaa agaaaggcac tgttgcaaag ttagcgatga
     1261 ggcagccttt tgtcttattc aaaggcctta catttcaaaa actctgctta ccaggcgcat
     1321 ttcgcccagg ggatcaccat aataaaatgc tgaggcctgg cctttgcgta gtgcacgcat
     1381 cacctcaata cctttgatgg tggcgtaagc cgtcttcatg gatttaaatc ccagcgtggc
     1441 gttgattatc cgtttcagtt tgccatgatc gcattcaatc acgttgttcc ggtacttaat
     1501 ctgtcggtgt tcaacgtcag acgggcaccg gccttcgcgt ttgagcagag caagcgcgcg
     1561 accataggcg ggcgctttat ccgtgttgat gaatcgtggg atctgccact tcttcacgtt
     1621 gttgaggatt ttacccagaa accggtatgc agctttgctg ttacgacggg aggagagata
     1681 aaaatcgaca gtgcggcccc ggctgtcgac ggcccggtac agatacgccc agcggccatt
     1741 gaccttcacg taggtttcat ccatgtgcca cgggcaaaga tcggaagggt tacgccagta
     1801 ccagcgcagc cgtttttcca tttcaggcgc ataacgctga acccagcggt aaatcgtgga
     1861 gtgatcgaca ttcactccgc gttcagccag catctcctgc agctcacggt aactgatgcc
     1921 gtatttgcag taccagcgta cggcccacag aatgatgtca cgctgaaaat gccggccttt
     1981 gaatgggttc atgtgcagct ccatcagcaa aaggggatga taagtttatc accaccgact
     2041 atttgcaaca gtgcctcact agtgaggggc ggcagcgcat caagcggtga gcgcactccg
     2101 gcaccgccaa ctttcagcac atgcgtgtaa atcatcgtcg tagagacgtc ggaatggccg
     2161 agcagatcct gcacggttcg aatgtcgtaa ccgctgcgga gcaaggccgt cgcgaacgag
     2221 tggcggaggg tgtgcggtgt ggcgggcttc gtgatgcctg cttgttctac ggcacgtttg
     2281 aaggcgcgct gaaaggtctg gtcatacatg tgatggcgac gcacgacacc gctccgtgga
     2341 tcggtcgaat gcgtgtgctg cgcaaaaacc cagaaccacg gccaggaatg cccggcgcgc
     2401 ggatacttcc gctcaagggc gtcgggaagc gcaacgccgc tgcggccctc ggcctggtcc
     2461 ttcagccacc atgcccgtgc acgcgacagc tgctcgcgca ggctgggtgc caagctctcg
     2521 ggtaacatca aggcccgatc cttggagccc ttgccctccc gcacgatgat cgtgccgtga
     2581 tcgaaatcca gatccttgac ccgcagttgc aaaccctcac tgatccgcat gcccgttcca
     2641 tacagaagct gggcgaacaa acgatgctcg ccttccagaa aaccgaggat gcgaaccact
     2701 tcatccgggg tcagcaccac cggcaagcgc cgcgacggcc gaggtcttcc gatctcctga
     2761 agccagggca gatccgtgca cagcaccttg ccgtagaaga acagcaaggc cgccaatgcc
     2821 tgacgatgcg tggagaccga aaccttgcgc tcgttcgcca gccaggacag aaatgcctcg
     2881 acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga
     2941 acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca
     3001 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     3061 atggcttgtt atgactgttt ttttgtacag tctatgcctc gggcatccaa gcagcaagcg
     3121 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     3181 agtcgcccta aaacaaagtt aacctctgag gaagaattgt gaaactatca ctaatggtag
     3241 ctatatcgaa gaatggagtt atcgggaatg gccctgatat tccatggagt gccaaaggtg
     3301 aacagctcct gtttaaagct attacctata accaatggct gttggttgga cgcaagactt
     3361 ttgaatcaat gggagcatta cccaaccgaa agtatgcggt cgtaacacgt tcaagtttta
     3421 catctgacaa tgagaacgta ttgatctttc catcaattaa agatgcttta accaacctaa
     3481 agaaaataac ggatcatgtc attgtttcag gtggtgggga gatatacaaa agcctgatcg
     3541 atcaagtaga tacactacat atatctacaa tagacatcga gccggaaggt gatgtttact
     3601 ttcctgaaat ccccagcaat tttaggccag tttttaccca agacttcgcc tctaacataa
     3661 attatagtta ccaaatctgg caaaagggtt aacaagtggc agcaacggat tcgcaaacct
     3721 gtcacgcctt ttgtgccaaa agccgcgcca ggtttgcgat ccgctgtgcc aggcgttaaa
     3781 catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     3841 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     3901 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     3961 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     4021 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     4081 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     4141 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     4201 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     4261 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     4321 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     4381 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     4441 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     4501 ggcctcccgc gcagatcagt tggaagaatt tgttcactac gtgaaaggcg agatcaccaa
     4561 ggtagtcggc aaataatgtc taacaattcg ttcaagccga cgccgcttcg cggcgcggct
     4621 taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag
     4681 tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca
     4741 tgaaaggctg gctttttctt gttatcgcaa tagttggcga agtaatcgca acatccgcat
     4801 taaaatctag cgagggcttt actaagcttg ccccttccgc cgttgtcata atcggttatg
     4861 gcatcgcatt ttattttctt tctctggttc tgaaatccat ccctgtcggt gttgcttatg
     4921 cagtctggtc gggactcggc gtcgtcataa ttacagccat tgcctggttg cttcatgggc
     4981 aaaagcttga tgcgtggggc tttgtaggta tggggctcat aattgctgcc tttttgctcg
     5041 cccgatcccc atcgtggaag tcgctgcgga ggccgacgcc atggtgacgg tgttcggcat
     5101 tctgaatctc accgaggact ccttcttcga tgagagccgg cggctagacc ccgccggcgc
     5161 tgtcaccgcg gcgatcgaaa tgctgcgagt cggatcagac gtcgtggatg tcggaccggc
     5221 cgccagccat ccggacgcga ggcctgtatc gccggccgat gagatcagac gtattgcgcc
     5281 gctcttagac gccctgtccg atcagatgca ccgtgtttca atcgacagct tccaaccgga
     5341 aacccagcgc tatgcgctca agcgcggcgt gggctacctg aacgatatcc aaggatttcc
     5401 tgaccctgcg ctctatcccg atattgctga ggcggactgc aggctggtgg ttatgcactc
     5461 agcgcagcgg gatggcatcg ccacccgcac cggtcacctt cgacccgaag acgcgctcga
     5521 cgagattgtg cggttcttcg aggcgcgggt ttccgccttg cgacggagcg gggtcgctgc
     5581 cgaccggctc atcctcgatc cggggatggg atttttcttg agccccgcac cggaaacatc
     5641 gctgcacgtg ctgtcgaacc ttcaaaagct gaagtcggcg ttggggcttc cgctattggt
     5701 ctcggtgtcg cggaaatcct tcttgggcgc caccgttggc cttcctgtaa aggatctggg
     5761 tccagcgagc cttgcggcgg aacttcacgc gatcggcaat ggcgctgact acgtccgcac
     5821 ccacgcgcct ggagatctgc gaagcgcaat caccttctcg gaaaccctcg cgaaatttcg
     5881 cagtcgcgac gccagagacc gagggttaga tcatgcctag cattcacctt ccggccgccc
     5941 gctagcggac cctggtcagg ttccgcgaag gtgggcgcag acatgctggg ctcgtcagga
     6001 tcaaactgca ctatgaggcg gcggttcata ccgcgccagg ggagcgaatg gacagcgagg
     6061 agcctccgaa cgttcgggtc gcctgctcgg gtgatatcga cgaggttgtg cggctgatgc
     6121 acgacgctgc ggcgtggatg tccgccaagg gaacgcccgc ctgggacgtc gcgcggatcg
     6181 accggacatt cgcggagacc ttcgtcctga gatccgagct cctagtcgcg agttgcagcg
     6241 acggcatcgt cggctgttgc accttgtcgg ccgaggatcc cgagttctgg cccgacgccc
     6301 tcaaggggga ggccgcatat ctgcacaagc tcgcggtgcg acggacacat gcgggccggg
     6361 gtgtcagctc cgcgctgatc gaggcttgcc gccatgccgc gcgaacgcag gggtgcgcca
     6421 agctgcggct cgactgccac ccgaacctgc gtggcctata cgagcggctc ggattcaccc
     6481 acgtcgacac tttcaatccc ggctgggatc caaccttcat cgcagaacgc ctagaactcg
     6541 aaatctaacg tccgttcggg catcgaggtc catgtcgggg tgggacgggc ccgtggcttc
     6601 aagatcactt gcagtccgac cgcgatgtct tggttgcgcg agaggttgtc gatatctgtt
     6661 gatttgcacc caaatttgac ccgggatttg cattgaattt tgacccaccc cttgttgtca
     6721 gaattatgtc tcgattttca gtttgcgggt ctgtttttcc tcctgcttat tctgagttga
     6781 actgtgtttg aagcggtaac tttcattgcc ggtttccagg atgtggcagt ggtgggttag
     6841 tcggtccaac aacgctgttg tcatcttttc atcgccaaac actcggctcc attccgagaa
     6901 gctcaagttg gtggtcagta tcacgctggt tttttcgtac agctttgaga gcaggtgaaa
     6961 cagcagtgcc ccaccggttt ggctaaaagg caaatatccc agctcatcca gaatcaccaa
     7021 atcggcatac aacagacggt ttgcgatttg tccctgacgc ccagatgatt tctcttgctc
     7081 cagtgcattg accaaatcca cggtggagaa gaaacgcacc cgtcggttca agtgcatcac
     7141 tgcttgtgta ccaatggctg tggccaggtg agtcttgcct gtgcctggcc caccaatcag
     7201 caccacgttc tgggcttgtt ccatgaagtc gcaccggtgc aattgtttga ccgtggcctc
     7261 attaaccagg ctttgactga agtcaaagcc caccaagtcc cgatacacgg ggaacttggc
     7321 cacccgcaat tgatagttca ccgaacgtac ttcacgctct gccacttcag ctttaatcaa
     7381 gctgtccagc atgggcaagg cttgattaaa tgctggtgaa ttctgattgc ccaactcctc
     7441 aatggcgtgt gccatgccaa agagtttcaa ggatttgagg attctcacat ggccttcatg
     7501 ctgcatcatg ggctctcctt aaactgtcat agcggttcac gttggcctgt ggttccaatg
     7561 tcagccttaa ccccttggga attggaatcg gtttgggtgg aggttcttcg gtcaaacgtc
     7621 ccaacagatt aagcacatgc tccttcgatg gcttgccaca ctccaatgcc aattccacag
     7681 cactgagtac cgcaccttca tcgtggtgca atacaagggc cagaatttcc accatgtcac
     7741 ggtcaccgcc ggggcgttgc agcaagatgg attgaagctt cttgaacgcg ggtggcaatt
     7801 cagcaaatgg cgcaccattg cgcaacgccc caggtttctt ctgaagcaca gacaagtaat
     7861 ggtgccagtc gtattgtgtg tggccacgcc gagcgtggcc actgccaaac aatcttggat
     7921 gctcggcaat gtgttggcct tcggcagcca tcaccagctt gtctgcataa atccgaaggc
     7981 tgatggccct gttggcgtaa ctggcaggaa cgctgtagcg attgccctcg tggtgaacaa
     8041 ggcaggttga agtgactcgc ttggtttgct ccacgaatgc atcaaaggca ttgggtagcg
     8101 ccatcaactc gccttgttca tcggcaaagg cctcttgcac ggtttggtcc aattcggggt
     8161 ggcgcagctc agaccacagc gctttgcagc gatgctcaag ccacacattc aaatcagcaa
     8221 ggctttgaaa gtctggtgcc ccttgccaca ggcgttggcg ggaatcctgc acgttcttct
     8281 caatctggcc tttctcccaa cccgatgctg gattacagaa ctgcgcatca aacaggtagt
     8341 ggctgaccat ggcagtgaac cgctgattga ccctgcgctc tttgccacgc cccaccgaat
     8401 ccacagcggt cttcatgttg tcgtagatgc cgcgcttggg aatgccaccg aagatttgaa
     8461 aggcatgcca gtgggcatca aacagcattt catgtttttg ctggtagtaa gcccgaagca
     8521 caaaggcccg gctgtgggcc aacttaaact gggcaatctg aagtttgacc tgtttgcccg
     8581 ctatgcgggc aaagtcctca ctccaatcga attggaaggc ttcgccacaa gcaaagcgca
     8641 aggggatgaa acaacccttg cccgaggttt gcgccttgaa ctgttcggaa tctttccact
     8701 gtcgggcaaa ggcacacact cggtcataag acccggtaaa gcccaaagcg accaaatccc
     8761 ggtacatgct gcgcaggttt ctgcgcagct tctttgtctt tttgtgctcg gtggagagcc
     8821 actgccttaa cttgggctca aaaggactta atttgccaac gctgtctcgc gctgggtact
     8881 gcggttcaac caccttgctt tgcaaatact tgcgaacggt gttcctggac aggccgcttc
     8941 gtcgggctat ttcccgaatc gacgcaccat cgcgaaaatg ccagcgtcga attgcgctca
     9001 atatcgccac gtttatcact ccttgatttc tcccgccata tccagacggg aaacagtgtc
     9061 atacgtgggt caaatttcga cgcaaatctt taccctaagt tggggtgcgg acaaaatctt
     9121 ggactacttt aggagtagtt catgtattcg tatgaagatc gccttcgagc cgtgaggttg
     9181 tacctgaagc ttgggcgccg gatgagcgcc acactacggc agctgggata ccccaccaag
     9241 aactcgctga aggcctggtt ggcagaattc gaacggaatc aggatcttcg ccgaggctat
     9301 caacggataa aacggcagta caccgatgag caaaagcaac gggcagtaga tcactatatc
     9361 gaacaaggct actgcctgag tcacacaatc cgaagcctgg gctacccaag ccgcgaggcc
     9421 ttgcgtgcct ggatccgtga tttacgccct gaattcgcta ggacggtcgt cggcagcagc
     9481 gctcccacag tcgcccgctc tcgcctcgag aagcagcaag ccgtcattgc actgaacctg
     9541 cgcgtaggtt cggcaaagga tgtggccgac actgtcggtg tatcgcgacc aacgttgtat
     9601 aactggcagc atcgattact tggcaaagtg cccctaaaac ccatgacaaa gaagaaaggt
     9661 gacacctcgc tcgagcagcg gcatgaggca ctactcaggg aactggccga actggagagc
     9721 cagaaccagc ggcttcgcat ggagaatgca attctggaga aggcgagtga attgataaaa
     9781 aaagacatgg gcatcaaccc cctcgaactg acaagccgag aaaaaacgaa ggtggttgat
     9841 gccctcagag tcacgtttcc attagccaat ctgttgtgcg gcctgaagct ggcgcgcagc
     9901 acatacttct atcaacgcct gcggcagacg cggcccgaca agtacacgca ggtgcgtgag
     9961 gtcattcgga ctatcttcga ggacaactac cgctgctatg gctatcgacg cattgatagt
    10021 gccttgcgcc ttggtggcat gcgtgtgtcc gagaaggtcg tgcgtcgctt gatggcgcaa
    10081 gagcgtctgg tcgtgagaac accgcgccgc cggcgcttct cggcgtatgc tggcgacccg
    10141 acaccagcgg tcccgaatct gctgaatcgc gactttcacg cgtcggcgcc gaatacgaaa
    10201 tggttgaccg atctgacgga aatacacatt ccggcaggga aggtctacgt ctcgccgatc
    10261 gtcgattgct tcgatgggct ggtggtggcc tggaatatcg gcaccagccc ggatgcgaac
    10321 ctggtcaata ccatgctgga tcacgcggta cggacactgc gacccggtga gcatccggtt
    10381 atccattcgg acaggggctc gcattatcgc tggcctgcgt ggatccgccg cactgaaaat
    10441 gcccaattaa cgcggtcgat gtccaaaaag ggctgctcgc cagacaatgc tgcatgcgag
    10501 ggctttttcg gacgattgaa gaccgaacta atctacccga ggaattggca gcacgtgacg
    10561 ctgaaagacc tcatgacgcg aatcgatgcc tatatccact ggtacaacga gcgccgcatc
    10621 aaagtgtcgc ttggcgggcg tagtcccatc gagtatcgtc atgcggtcgg attgatgtcc
    10681 gtataaaccg tccaagaaat cgtccgcacc cccagtgggt caattttaga tgcaactcaa
    10741 caggccatgc tgagtgtgcg atggttgatc gcttcctcgc cgctctccac ggcgacgatg
    10801 gccgccgcca tcagcaagtg cgccagttcc cctatggtgc cctcgctgcg tgtgagcagg
    10861 tagcgagcca tgtccagcgt ggcaattggg gaaggccggc gcagcgggag cgaagcggcg
    10921 aagctggcca gcagtgagca gcaatcgtcg ttggcctccc ataccggcag catcatcggc
    10981 tcgaagcgat tttccaactg gtcatcggag cggatggcta ggtaggcgtc gcgcgtgcct
    11041 accccaacca acgggatgcg cagttcgttg ccgaggaagc gcagcaggtt gaggaattcc
    11101 cggcggttga cgctgttgcc ggccagcacg ttgtgcagct cgtcgatcac cagcatgcgc
    11161 acgccgacct tgcgcagcag tgccagagcc agttgctcca tttccggcaa ccgtgggcgt
    11221 gggcgcagcg gcgcgcccat cgcggcgagc agcgcgacgt agaagcggat cacggacggc
    11281 tcggacggca tctgcacgac caacaccggg atgtgctcct ggtcggcgtc ggagctggcc
    11341 gggtgggtgc ggcggaactt ctcgacgatc atcgacttgc cattgttggt cgggccaacc
    11401 agcagcaggt tgggcatgcg ttgcttgttt ggccacgcat aaagggcttc cagccggttc
    11461 agcgcctcga ctgcgcgcgg atagccgatc cagcggtcgg cgcgaaggcg ctggatgcgc
    11521 tcgtccgccg gaagacgggc caagccctgg gccgccggca gcaggtggga caggtcgatg
    11581 atgggatatt cgtccacggc taccactcct caatctggtc gaacggtttg gcgggtggca
    11641 agttgtctgc ctgcgggtcg gcaatatccg tatccggcgg aacgggcttg tccggccgag
    11701 ctgatgtctt gaggtgctgg cggcgatccg cgtcacgccg cgccttgcgt gtggccttct
    11761 gcgcgctggt cacaatctca cgcatctggc cgatcatgcg gaacagcgcc gactcatcca
    11821 cctgttcgcg cccttgctgc cgcagtttcg ccagcgcctg ccgttgttcc cagagggtga
    11881 cagccggatg cgacaaggta cggtagggaa tttccaggta atgctgtccc tccggttcca
    11941 ggacccagat acggctgatg tcgcgcggat cgcgccggat cagaaaggac ggccagcgtt
    12001 cacgccgcgc aatccacggc ttgagcgcat cggcgtagta gtggatgtgg tcgatgacaa
    12061 agccggtgcg ggtcagcgtg cgccggagga tcggcagaaa atcgaccagg aacgaagtag
    12121 cgcgtgtgac gacggccggt acgccgacac gcgccacggc ctcggcccag cgcgcggccg
    12181 gcggttggag caggccgttg tgcaccgaac cgtggtaggt gccgaccgcc aatgtgagcc
    12241 agcgctctag ctcgcgcagc gtcagggcgg ccttgttttc ggaatcgtag tcgccgcgct
    12301 ggtcagggtt ggagaaggtc gttcccggca gttcgtcgtg aatcatctgc atcgccgtgc
    12361 cgatgatccg ttccacgatg ccgccatagt gcggctgtcc cagcgggcga tagtccagcc
    12421 ggatgccatg ctgctcgcaa ccccggcgca gggcctcgct cttgaactcg gccgcgttgt
    12481 ctaggtagag cagcaagggc ttgccgctca tctgccaatc catttccacg ttcagtcctt
    12541 ccagccaagg gcgcttgtcg caggcgacat gcacgaggca caggccaacc gaaacggcag
    12601 acggcgcttc cagcgtgacg accatgccga gcacgcagcg ggtgaacacg tcgatggcga
    12661 gggtcaggta cgggcggcca ataggttgcc ggtcgcggtc atcgaccacg atcaggtcga
    12721 tgaccgtatg gtctatctgc acctgctcca gcggcgcggt cacggcagga ggctcgccgc
    12781 ccacaccttg taggtcacga gcggcatcct ggccttcccg ccggcggatg accttgcgcg
    12841 ggtcaaggct agcgatccgt aaggccacgg tattgcgcgc cggcactcgc agtttttgag
    12901 ccttgcacac ctgagtgact tcgcggtgaa aggccgctag gctgcgcttc tgcttggtca
    12961 ggaaccgctt ttgcagtagc tcgtggatga cgcgctcgac cggttccggc aagcgcccct
    13021 tacctttacc tccaccggac tggccgggca ccagatccgt cacgaggccg ctgccttgcc
    13081 gggcacgccg gatcagaacg tatacctggc gccgagacaa gcccagcgcc tgagccgcca
    13141 tatcggccgc ttcgtgcccg accgtctccg actgcgccaa cggactgatg atctccgcac
    13201 gacggcgcgc acgctcccaa gcctcatcag gcagagtggc cacgccttgt tctggaatcc
    13261 gtggggtgtc cgtcgccatg ctcacctcgc tttggtgcac acgagtattg agcatagtcg
    13321 agattggtgc agatcacttc tgatattgaa ctgtcaggag ctggctgcac aacagccatt
    13381 acgcccaatc aactggtgca gtcgtcttct gaaaatgaca tccatgccca gcccgtgcgc
    13441 gagctggatc accgcccgca cgatagtttg gtcacgggca tcatccggga gcctggcgac
    13501 aaaggattgg tcgattttca atgtggtgat ggggcagcat ttcagatgtt gcaggcagga
    13561 atagccggtg ccgaagtcgt cggcggcgaa gcgcacgccg atggcgcgca aggcgtcgaa
    13621 actggcgaac agggctggat tgccgaatgc gaccgattcg gtcagttcga tctccagaag
    13681 ctcggcgggc agggccatat cggccagcac ccgctttacc tcgtcgtcga acgttggccc
    13741 aacctggctg gcggacacat tgatggcaag acggaacggt tgccatgccg gtccttgcca
    13801 cttgtgcatc tggcgacagg cctcgcccag cacccacgcg cctatttccg gcatcaggcc
    13861 gaacgactcg gccagcggca ggaactggcc gggcggcaac aggccaagcc tcggatgccg
    13921 ccagcgcatc aacgcttccg cgccagcgat ccggtgatcg cgcagatcga ccagcggctg
    13981 gtaatgcagg tcaagctgtc cgcgcgccgc cgcctgcgcc aactcggccg ccgtccatcc
    14041 ggcgggctgc gaactcgtca tgatccgccc cggaaggcgc gcagcagccg cgttacggcc
    14101 agaacgaaca agccggtcag cgcgagcgcg gcaacacccc aatgctcgcc aaggaaggca
    14161 ccggcggtcg tcccggccag cacggcggcg agaatcggca gatggcaggg gcaggtcaac
    14221 acggccagcg caccccacag gtagccggaa acgggttggc gcgtctcggg cggcagtttg
    14281 tcaggggcgt tcacggcaat gcctcctcgt gcgcccgctc ggctggcatg gaggccagtt
    14341 gcgcgtccag atgggccaac gccgcgcgcc gccgctcgac caactggcgc agcacggcaa
    14401 gctgcgctgc ggcttgtgcg ccgtccgctg cgtcgagcgc acggcacagc cgcgccaggg
    14461 catccaggcc gatacccgcc tcgaaggccg cgcgcacgaa gcacagccgt tgcaaggccg
    14521 catcgtcgaa cacgccgtag ccgcccgtgg tgcaggccac cggccgtaac aagccgcgca
    14581 ccaggtagtc gcgcacgata tgtacgctca ccccagcgtt atgggccagt tgcgataccg
    14641 tgtaggcgct catcgcacac ctccttgtcc tcacccggcg cagcaggaaa gctgcttcac
    14701 atccttgttg aaggtctgcg ccgcgagctt caacccttcg accatcgtca ggtaggggaa
    14761 caactggtcg gccagttcct gcaccgtcat ccggttgcga atcgccagtg cggccgtctg
    14821 gatcagttcg cccgcttccg gggccactgc ctgcacgccg atcagtcgtc cgctgccttc
    14881 ttcaaccacc agtttgatga agccgcgcgt gtcgaagttg gcgagcgcgc gcggcacgtt
    14941 gtccagcgtt agcgtgcgac tatcagtttt gatgccgtca tggtgcgctt ccgcctcgct
    15001 gtagcctacg gtcgccactt gcgggtcggt gaacaccacg gccggcatcg cggtcaggtt
    15061 cagggccgcg tcaccgccgg tcatgttgat cgcggcgcga gtgccggccg ctgccgccac
    15121 atagacgaac tgcggctggt cggtgcagtc gcctgcggcg tagatgtgtt ccacgcttgt
    15181 acgcatgccg gggtcgatga cgatagcgcc ttgcggggtg agcgtgacgc ccgtcgcatc
    15241 cagtgccagc ttgcgtgtgt tgggcgcgcg gccggtggcg accagcagct tgtcggcgcg
    15301 cagttcgccg tgcgccgtgg tgagcacgaa ttcgccgtcc ccttcaccat tgatatacgc
    15361 gacctggctg gcctgggtgt gttccctcac ctcgatgccc tccatgcgga atgcggccgt
    15421 gacggcttcg cctatagctg ggtcttcgcg gaagaacagc gtgctgcgag ccaggatcgt
    15481 caccttcgct ccgagtcggg cgaacgcctg cgccagctcc agcgccacca ctgatgagcc
    15541 aatcacggcc aggcgcttag gaatcgtctc gctgaccagc gcttcagtgg aagtccagta
    15601 cggagtgtct ttcaggccgg gaatcggcgg cacggccggg ctcgcgccgg tggcgatcag
    15661 gcagcggtcg aatgccacca cgcgctcgcc gccgtcgttg agttgcacga tcaggttgcg
    15721 attgtcctta aagcgggcgg agccgtgcag cacagtgatc gccggattgc cctccaagat
    15781 gccttcgtac ttggcgtggc gcagttcatc gacgcgggcc tgctgctggg ccagcagcgc
    15841 cgtgcgctgg atggtcggcg tggtagcggc gatgccgcca tcgaacgggc tttcccggcg
    15901 cagatgggcg atatgggcgg cgcggatcat gatcttggac ggcacacaac cgacattgac
    15961 gcaggtgccg ccgatggtgc cgcgctcgat cagcgtgaca cgtgcgcctt gctcgacggc
    16021 cttcagcgcc gctgccatcg cggccccgcc gctgccgatg acggcgatat gcaatgcgcc
    16081 gctgctaccc gtcttgtcgt ttctgcccag cagatcgcgc atcttgtcga gcaatccgcc
    16141 cggcgtcgaa actgaggggg catcggccag cgtggcccga taaccgagtc cagctacagc
    16201 ggccgtcagc gcgtcgggtg acgtgccgac ctcaatggcg agcttggcgc tgcccttggc
    16261 gtaggagaca tccgctgatt gcacgccggg cactttctcc agggcgtcct tgacatgcac
    16321 tgcgcacgag tcgcaagtca tgccggtgat tttgagagtg ctcataccat cgttccttat
    16381 tcgtgtgggc cgccgtgtcg cacggtcagc cgtctttcac aagcgcttgg cggggagttc
    16441 gcagccgtcc ggtccgcaac ggcgatgcgc cggcgacacg aagtcccaga tcgacacccc
    16501 aatcatcaag gccaggccga cgtacatcag gttcgccgtc caccagttgc cgagcagcca
    16561 gaccgtggcc gcaaacacga tggccgggcc gatcatgccg agcagactgc gcagccattg
    16621 ccgatgactg aaccaaccca gcgcgttcgc caggaaggcc agcgcggcaa acagcggcag
    16681 caggcggctg atgaacagtc cctcgtactg gctcaagaag cccagcccga tggccgcgcc
    16741 gaagctggcg agggctggaa agcaggcggc gcagcccatc gcggaaacga cgctgccgag
    16801 cgcgccggtt ttatcggcaa tgcgtgtcat cagtcccatg aagcggctct cgctgttgtc
    16861 gttggcttgc tggctcactg cttgacgctg gacggatagc cggcgtctgc ggtggccttg
    16921 gtcagcttct gtacgctggc cttggtgtcg tcaaaagtga cgacggcctc gcgcttctcg
    16981 aagcccacat cgaccttgct cacgccttcg accttggaga gcgctttctt gactgtgatc
    17041 gggcaggcgg cgcaagtcat gccgggaacc gctagcgtga cggtctgggt agcggcccac
    17101 accggggcaa cagcggcggc gagggcaagg gaggcaaaca gtttcttcat gatgaactcc
    17161 tggttaatag aaaaatggaa cgacataggg aaatccaagc gcgaccagga ccagcacggc
    17221 cacgatccag aaaatcagct tgtaggtggc gcgcacctgc ggaatcgcgc agacctcacc
    17281 tggcttgcat gcctgcacgg gccggtaaat ccgcttccag gcgaagaaca gcgccactag
    17341 cgccgcgccg atgaacaacg gtcgataggg ttccagcacc gtcaggttgc cgatccaagc
    17401 accggagaag cccagggcga ccagtactag cggccccagg cagcaggtcg atgcaagaat
    17461 ggcggccagc ccgccggcga agagcgcacc gcgcccgttt tgtggttcag acatacgttg
    17521 gcccttttga atttggattg gatagcgtaa ccttacttcc gtactcatgt acggagtcaa
    17581 gcgatatgga aaataatttg gaaaacctga ccattggcgt ttttgccaag gcggccgggg
    17641 tcaacgtgga gacaatccgc ttctatcagc gcaagggcct gttgcgggaa ccggacaagc
    17701 cttacggcag catccgccgc tatggggagg cgggtaatga ctccaactta ttgatagtgt
    17761 tttatgttca gataatgccc gatgactttg tcatgcagct ccaccgattt tgagaacgac
    17821 agcgacttcc gtcccagccg tgccaggtgc tgcctcagat tcaggttatg ccgctcaatt
    17881 cgctgcgtat atcgcttgct gattacgtgc agctttccct tcaggcggga ttcatacagc
    17941 ggccagccat ccgtcatcca tatcaccacg tcaaagggtg acagcaggct cataagacgc
    18001 cccagcgtcg ccatagtgcg ttcaccgaat acgtgcgcaa caaccgtctt ccggagcctg
    18061 tcatacgcgt aaaacagcca gcgctggcgc gatttagccc cgacgtatcc ccactgttcg
    18121 tccatttccg cgcagacgat gacgtcactg cccggctgta tgcgcgaggt taccgactgc
    18181 ggcctgagtt ttttaaatgg cggaaaatcg tgttgaggcc aacgcccata atgcgggcgg
    18241 ttgcccggca tccaacgcca ttcatggcca tatcaatgat tttctggtgc gtaccgggtt
    18301 gagaagcggt gtaagtgaac tgcagttgcc atgttttacg gcagtgagag cagagatagc
    18361 gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc gtcagtagct gaacaggagg
    18421 gacagctgat agaaacagaa gccactggag cacctcaaaa acaccatcat acactaaatc
    18481 agtaagttgg cagcatcacc gaaacaccgg acctggatca ctgaagcttt acgtcttcac
    18541 tttgaagaac atttaccccg ggttgt
//
LOCUS       DAZGLJ010000079        18512 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000079 DAZGLJ010000000
VERSION     DAZGLJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.079"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            96..1316
                     /locus_tag="ACHK49_002408"
     CDS             96..1316
                     /locus_tag="ACHK49_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO9263043.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1289..1918
                     /locus_tag="ACHK49_002409"
     CDS             1289..1918
                     /locus_tag="ACHK49_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO9263044.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1919..3079)
                     /gene="ybdL"
                     /locus_tag="ACHK49_002410"
     CDS             complement(1919..3079)
                     /gene="ybdL"
                     /locus_tag="ACHK49_002410"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO9263045.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSEVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3188..4276
                     /gene="hcxA"
                     /locus_tag="ACHK49_002411"
     CDS             3188..4276
                     /gene="hcxA"
                     /locus_tag="ACHK49_002411"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO9263046.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4286..4483)
                     /locus_tag="ACHK49_002412"
     CDS             complement(4286..4483)
                     /locus_tag="ACHK49_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO9263047.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4665..6770)
                     /gene="cstA"
                     /locus_tag="ACHK49_002413"
     CDS             complement(4665..6770)
                     /gene="cstA"
                     /locus_tag="ACHK49_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO9263048.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6951..7364)
                     /gene="entH"
                     /locus_tag="ACHK49_002414"
     CDS             complement(6951..7364)
                     /gene="entH"
                     /locus_tag="ACHK49_002414"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO9263049.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7367..8113)
                     /gene="entA"
                     /locus_tag="ACHK49_002415"
     CDS             complement(7367..8113)
                     /gene="entA"
                     /locus_tag="ACHK49_002415"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO9263050.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8113..8970)
                     /gene="entB"
                     /locus_tag="ACHK49_002416"
     CDS             complement(8113..8970)
                     /gene="entB"
                     /locus_tag="ACHK49_002416"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO9263051.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8984..10594)
                     /gene="entE"
                     /locus_tag="ACHK49_002417"
     CDS             complement(8984..10594)
                     /gene="entE"
                     /locus_tag="ACHK49_002417"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO9263052.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10604..11779)
                     /gene="entC"
                     /locus_tag="ACHK49_002418"
     CDS             complement(10604..11779)
                     /gene="entC"
                     /locus_tag="ACHK49_002418"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO9263053.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12148..13104
                     /gene="fepB"
                     /locus_tag="ACHK49_002419"
     CDS             12148..13104
                     /gene="fepB"
                     /locus_tag="ACHK49_002419"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO9263054.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(13108..14358)
                     /gene="entS"
                     /locus_tag="ACHK49_002420"
     CDS             complement(13108..14358)
                     /gene="entS"
                     /locus_tag="ACHK49_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO9263055.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14469..15473
                     /gene="fepD"
                     /locus_tag="ACHK49_002421"
     CDS             14469..15473
                     /gene="fepD"
                     /locus_tag="ACHK49_002421"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO9263056.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15470..16462
                     /gene="fepG"
                     /locus_tag="ACHK49_002422"
     CDS             15470..16462
                     /gene="fepG"
                     /locus_tag="ACHK49_002422"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO9263057.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16459..17274
                     /gene="fepC"
                     /locus_tag="ACHK49_002423"
     CDS             16459..17274
                     /gene="fepC"
                     /locus_tag="ACHK49_002423"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO9263058.1"
                     /translation="MNESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17271..18404)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHK49_002424"
     CDS             complement(17271..18404)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHK49_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO9263059.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4355 a         5241 c         4784 g         4132 t
ORIGIN      
        1 aatgaaaaat aaaacactct gctttgcagt gtttacttta taattaacag acttatctca
       61 ctgatcaccc tgtaacgttc agagagcgtc ttcagatgtc tatttataaa attcctcttc
      121 cgctcaatat tctggaagct gcacgagagc gtattacctg gacactcaac accctacccc
      181 gcgtttgtgt ctcattttct ggcggcaaag actctggttt aatgctgcat ctgacagccg
      241 aacttgcccg acaaatgggc aaaaaaatct gcgttttgtt tatcgactgg gaggcgcaat
      301 tctcttgcac cattaactat gttcagtccc tgcgcgagtt gtacaccgat gtcatcgaag
      361 agttttactg ggttgcgctc ccgcttacga cgcaaaattc cctttcacaa taccaacccg
      421 aatggcagtg ctgggaacct gatgtcgaat gggtgcgtca gcccccgcaa gatgcgataa
      481 ccgatcctga ctttttctgc ttttaccagc caggcatgac cttcgaacaa tttgtacgtg
      541 agtttgccga atggttttca caaaaacgtc cggcggcgat gatgatcggc atccgtgcgg
      601 atgagtccta caaccgtttt gtcgccatcg ccagtttaaa taaacaacgt tttgccgacg
      661 ataaaccatg gactacggcc gcaccaggcg gtcatagctg gtacatttac cccatttacg
      721 actggaaagt ggctgatatc tggacctggt atgcaaatca tcaaaatctc tgcaacccac
      781 tgtataacct aatgtatcag gcaggcgttc ctctgcgtca tatgcgaatt tgcgaacctt
      841 ttggcccgga gcaacgacaa ggattatggc tctatcacgt tatcgaaccg gatcgctggg
      901 ctgctatgtg cgcacgagtc agcggggtaa aaagtggcgg catttacgcc ggacatgata
      961 atcatttcta tgggcatcgg aaaatcctca agccagaaca tttagactgg caagaatatg
     1021 cattattgct gctcaatagc atgccggaaa aaacagctga gcattaccgc aataaaatcg
     1081 ccatttattt gcactggtat cagaaaaaag gcatcgaggt tccacaaacc cagcaagggg
     1141 acattggcgc gaaagatatc ccctcctggc ggcggatatg caaagtttta ctcaataacg
     1201 attactggtg tcgggcatta tcatttagtc ctacgaaagc gaagaactat cagcgttata
     1261 acgaacggat aaaaggaaaa cgtcaggaat gggggatact atgcaacaac gattaacgca
     1321 ggatttaacg caatttctcg ccagcttgcc ggaagatgat cgcatcaaag cgattaatga
     1381 aatccgcatg gcgatccatc aggttagccc ctttcgtgaa gagccagtgg attgcgttct
     1441 gtgggttaaa aacagccaac ttatgcctaa tgattacaac ccaaacaacg tggcaccacc
     1501 cgaaaaaaaa ctgctgaaaa aatccattga aatagatggt tttacccaac ccattgtggt
     1561 cacgcatacg gataaaaatg ccctggaaat cgtggatggt tttcaccgcc atgaaattgg
     1621 taaaggctca tcatcattaa aattacgctt aaaaggttat ttgccggtga cctgtctgga
     1681 aggtacgcgt aatcaacgca tcgccgcgac tattcgtcat aatcgcgctc gtggacgcca
     1741 tcaaatcacc gccatgtcgg aaatcgtccg tgaacttagc cagttgggat gggacgataa
     1801 taaaattggc aaagagctag gaatggacag cgacgaagtg ctgcgcctga agcaaattaa
     1861 cggcctgcaa gagctgtttg ccgaccgtca atattcccgc gcctggacgg ttaaataact
     1921 aaagctggcg caggcgttca gctgctgcca gcaacgtcga ttccttcttg gcaaaacaga
     1981 gacgaatcag tttatgtggg aagggatcgg cgcaaaacac cgacagcgga atcgccgcta
     2041 cgccgtgctc ctgcgtcagc cactggcaaa actcaacatc atccagggta gaaacctcgc
     2101 tgtaatccac cagcaaaaag tatgtacctt cacacggtaa aatctccagc cggctttcat
     2161 ttaaagcatt caccagaata tcgcgcttct ggcgataaaa gtccggtaac gcaagataat
     2221 gctcaggttc tgcacgtagc atatcagcaa gcgccagctg tgccggggta ttcaccgaaa
     2281 aggtcagata ctgatgtacc ttgcgaattt cggcgctgat gggcgctggc gcaacacaat
     2341 aacccacttt ccagccggtc atatgatagg tcttgccaaa tgaagaaacc gccactgccc
     2401 gctcacgcag ctgcggatgc gccagcacac tggcatggcc ctgttgtgaa aagttgatgt
     2461 gctcgtagac ttcatcgcta atgacaaaaa tctcgtgccc ggcgatcgcc tgccacaaag
     2521 cggcgaaatc agcctgctgc cagacagttg cactggggtt atgcggagtg ttgaggatca
     2581 ccagtctggt gcgctcgctt aataatgcgg caaattcctg ccagtcaacg cgaaaatgcg
     2641 gtggttgcag tgccatacgc ttcactattc ccccagaaag cgcgatggcg ggggagtaac
     2701 tgtcatagct gggatcaaaa caaatcactt catcgccatt gcgcaccagt gcggtaatcg
     2761 ccgcgtataa cgcttccgtc gcccctgccg ttacggtgat atcgctatcg gcatctggtt
     2821 gatagccata caaacgttcc gttttctgag caatcgcctc gcgcaaggcc tgcacgccgg
     2881 tcatgggcgc gtattggttt gccccctggg caacgtggta cgccagccgc tcctgtaaat
     2941 agcgcggacc atcaaaatca ggaaagcctt gcgacaggtt aatcgcctgg tgttgctgcg
     3001 ccagcgcgct catctgggtg aaaatagtgg tgccaagttg tggaagtttg ctttgtggaa
     3061 tcagagggtt atttgtcatt tttattgtac ctgacgaagt tgcgtgttga aggcactata
     3121 acacgatgct agtatttggc aatcaagacg tttagatgtc taaataaaac aataaggaca
     3181 acacaacatg cctcacaatc ctatccgcgt ggtcgtcggc ccggctaact acttttcaca
     3241 tccaggaagt ttcaatcacc tgcacgattt tttcactgat gaacaacttt ctcgcgcggt
     3301 gtggatctac ggcaaacgcg ccattgctgc ggcgcaaacc aaacttccgc cagcgtttgg
     3361 acttccaggg gcaaagcata ttttgtttcg tggtcattgc agcgaaagcg atgtacagca
     3421 actggcggct gagtccggtg acgatcgcag cgtggtgatt ggcgtcggcg gcggcgcact
     3481 gctcgacacc gcgaaagccc tcgcccgccg tctcggtctg ccgtttgttg ccgttccgac
     3541 gatcgccgcc acctgcgccg cctggacacc gctctccgtc tggtataacg atgccggaca
     3601 ggcgctgcat tatgagattt tcgacgacgc caattttatg gtgctggtgg aaccggagat
     3661 tatcctcaat gcaccgcaac aatatctgct ggcggggatc ggtgacacgc tggcgaaatg
     3721 gtatgaagcg gtggtgctgg ctccgcaacc agaaacgttg ccgctaaccg tgcgactggg
     3781 gatcaataat gcgcaggcca ttcgcgacgt attgttaaac agtagcgaac aggcgctgag
     3841 cgatcagcaa aatcaacagt taacgcaatc attttgcgat gtggtggatg ctattattgc
     3901 tggtggtggg atggtcggtg gtctgggcga tcgttttacg cgtgtggcgg cggctcatgc
     3961 cgtgcataac ggtctgactg tcctgccgca aaccgagaaa tttctccacg gcaccaaagt
     4021 cgcttatggc attctggtac aaagcgcctt gctgggtcag gatgatgtgc tggcgcaatt
     4081 aaccggggcg tatcagcgtt ttcatctgcc gaccacgctg gcggagctgg aagtggatat
     4141 caataatcat gcggagatcg acaaagtgat tacccacacc ctgcgtccgg tggaatccat
     4201 tcattacctg ccagtcacgc tgacaccaga tacgttgcgt gcagcgttcg aaaaagtgga
     4261 atcgtttaaa gcctgacgtg caaatttagc agcagcgcgc gccgcctttt ccaccgtagc
     4321 gcgcgtcctg ccgctcacgg aaaaactctt cgtaggtcat cggcgtttga tcgggatggt
     4381 taacccgcat atgttcgaca tagttgtcgt aatcaggcat accaatcatc agcttcgccg
     4441 cctgacctaa atattttccg gcttttgcca gtgaatcaaa catcttggcc tccgctaacc
     4501 atgttgctga aagagtgccg gatgcggcgt gaacgcctta tccggcctac aaatgctatc
     4561 aacgcgctcg gtcagtcccc tcgccccatc ggggagaggg ttagggtgag gaggaaccct
     4621 cggcactatg ccaacattcg ccaacatccc ccctcactct gactttagtg tgcgcctttt
     4681 gcctgtgcca cgatctcctc gacattttcc ggcattggct catacggcgt ttctttcgcc
     4741 gttggcttcg gatctttcaa tgccgccagt gccgtcttaa tcgagaacag cgccagaacc
     4801 accacgacca ccataaagaa gatggttaac ccggcatcca gacggttgtt gaacaccagt
     4861 tgcgccagct gtgactcagt atactgcgac ggaatattgc cgctgtcgat cattgcctgg
     4921 aacttattag caatggccag gaagccgact ttcgcatccg ggctaaacgc tttctgccag
     4981 cctgcggtca gggtacaaat cagcagccag gccgttggta ccagcgccac ccaggcgtaa
     5041 cgttgacgtt tcatcttgaa caacaccacg gcacagagca tcagcgccat ccctgccagc
     5101 atctggttgg caataccaaa cagcggccac agagtgttaa tgccgcccag cggatcgacc
     5161 acgccctgat ggaggaagta gccccacgcc agtacgcaca gcgctgttgc cagcaggtta
     5221 gcaggcagtg aatcggtccg cttcaggccc ggagaaacca cgcccagcag atcctgcaac
     5281 ataaagcgcg cagcacgcgt acccgcatct accgccgtca gaataaacag agcttcaaac
     5341 agaatggcga agtgatacca gaacgccaca tccatcatac cacccagcgc accgtgcagg
     5401 atgtaagcca tccccaccgc cagcgtcggc gcaccgcccg cacgggaaat gatcgactgt
     5461 tcacccactt cgctggcaat ctggttgagc gtatctggtg taatgctaaa gccccagcta
     5521 ctcaccacct gcgcggcaga agcgactaca tccgccgtcc ctgccggagc cagcaccgcc
     5581 attgggctgt tcatggcaaa atacacgccc ggatcgatga tacaggcaga aaccagcgcc
     5641 ataatcgcca cgaaggattc catcaacatc ccaccgtagc cgataaagca cgcctgccct
     5701 tcgttcgcca gcatcttcgg cgtggtgccg gaagagatca gcgcatggaa gccagacacc
     5761 gcaccacagg cgatggtgat aaacaggaac gggaacaggt taccggtcca taccgggcca
     5821 gtgccatcga caaatttggt cagcgcaggc atggtcagcg tcgggcgcat aatcaaaatg
     5881 cctaccgcca gaccgacgat agtcccgatt ttcaggaagg tcgagagata gtcacgcggg
     5941 gccagcagca accacaccgg cagcaccgcc gccacaaaac cgtagcccac cagcatccag
     6001 gtcagctgca cgccggtaaa gtcaaagtac ggtgcccagg tcggactttc tgccacccag
     6061 ccgccagaga taatggcgaa aatcaggaac accaggccaa tcactgacac ttcgccaatg
     6121 cggcccggac gcagatagcg caggtagatt cccataaaaa tcgccagcgg aatggtgaac
     6181 gcaacggtat acgttcccca cgggctatga gtcagggctt tcaccacgat catcgccagt
     6241 actgcaagga taatgaccat gatcataaag caggctacca gcgcaatcac cccggcagtt
     6301 ggccccatct cttctttgac cagctcaccc agcgatcgac cgtcacggcg cgtagaaaca
     6361 aacagcacca tgaaatcctg caccgcacca gcgagaacca ccccggcgag cagccagatc
     6421 atccctggca ggtaacccat ttgcgccgcc agtaccggcc caaccaacgg acctgctccg
     6481 gcaatggccg caaaatggtg accgaacagc actttcttgt ccgtcggcac atagtccagc
     6541 ccgtcgttat ggcgcaccgc tggcgtcata cgcgtcgggt caaccgccag cacatttttg
     6601 gcgatataca gcccataaaa acggtaagcg atcagataga tacagaccga cgccaccaca
     6661 atccacagcg cgttgatctg ttccccacga tttaaagcaa tgtatcccag agcaaatgct
     6721 cccattacag agagcactgt ccagacgagg tatttccctg atttgttcat agttgttatc
     6781 cgtgtgcgtg tccatagaga tgttacattt tgtttctata acatctttgt aagatttaac
     6841 aacacggtaa ccatgtaaat cggagttgaa accaatattt aacttaacaa tgttaactcg
     6901 atcactccgt taaccgagtt ttcctgcaat ctcatttaat tctgtcgggc tcatcccaaa
     6961 actgccgtac ccagccgaca agtgcagcaa cgccgcccct gttcatcgaa aacgacgatt
     7021 tcccagctct gattttgccg cccaagatgc agcggctggc agacgccgcg taccttgcct
     7081 tcagacaccg ggcgatgatg ggtggcgtta agttccgtac ccaccacaca ctgcccgtca
     7141 cgggtcatca taaatccggc catcgatccc agcgtttcag ccagcgccgc cgacgcgccg
     7201 ccatgcagca ggccaaacgg ctgatgagta cgggtatcaa ccggcatttc ggcttccagc
     7261 acatcatctc ccagacgggt atacacaatt cccagatgcg ccaccattgt gttatcgctg
     7321 gtggcgttca gttcgtcgag cgttaaatgg cgtttccaga tcatgcttat gcccccagcg
     7381 ttgagccgcc atcgaccaca atatcctgta gggtgatatg gctggcgagg tcagaggcga
     7441 ggaacaaaat cgtgttggcg atctcttgtg gacgggcgat tttccccagc ggaatgccga
     7501 gtttaaactg ctcgccaaag ccgcgaatac gctgttcttc ggcgtcatcg ctcacccaca
     7561 gcgtgcgttg catatcggtg tcggtggagc caggcgaaac cacattacag cgcacgccgc
     7621 tacccgccag ttccagcccg acgctcaacg ccaggctttt cagcgccgct ttcgatgcgc
     7681 cataagcact cataccaata cgcggcgtgt gtgcggcgtc ggacgccaca gtgacaatcg
     7741 ccccgccccg ctgacggcga aactggttca tggtttgctg gaacaggtta aacgcaccgc
     7801 cgacgttaac cgcaaaagtc tgctgccagt cctctttact gagctgatcg gtcgcgccca
     7861 tgcgcaaaat tcccgccgca ttgatcagca cgtccagtcg ctccgtttca gctaacagtc
     7921 gctgacatac ctgcgcgacc tgcgcagcat cggcaacatc catcacttcg gtcgcaaagg
     7981 gatattgctc ctgagcgaac gcttgatcaa aaccggtaac tttcgctccc gcctcaacaa
     8041 acgccagcgc cgtggcgtag ccgatacctt tacctgcgcc ggttacccag acatttttac
     8101 cgctgaaatc cattatttca cctcgcggga gagtagcttc caccaggcgt cgatggtcgg
     8161 gtttttcgcc agcatgacaa agtcgatatc accatgcact ttgcgccagc gcgccgccag
     8221 cgccatcatg cgcaccgaat ccagaccgta gtcgatcagg ttgtcgtcat cgaacggttc
     8281 atcggactcg tccagcaacg gcaggataac ctcacgcagc gccgctttgc tggcggggac
     8341 aggtgctggc agtaattctt cagtcatcac cacccggcca gaccgcccgg ccacatattt
     8401 cagcgacatc aaatgctcgt cacggctgaa atcggccagc gcatccgcca ccataaacgg
     8461 tttaatatcg cgcataaatg cgtcggttgc ggtggtcata cagccaatgt gggcatatac
     8521 cccggtaata atcagctggt tacgtccact ctctttgagc atttgctcca gcggagaacg
     8581 atgaaacgcg ctgtagcgcc acttcaccag caccgtgtcg tcggcatctg gcgtcaggcg
     8641 atccaccacc ttttgctgtt ccggcgaacg ggtcaggccc ggcccccaca tatcattcaa
     8701 cagcgcccga tcttcatcgc tctgctcttt cggctgggcg gtgtaataaa ccggaatatt
     8761 gtgctgtttg cagtagtcgc gcagcgcagc aatattcgcg atcacctgct ccatcatcgg
     8821 gcagttctcg ccccagaagc tgacaaaata gtcctgcata tcatggatta acaacgcggc
     8881 acgttgcggt tcaaaggccc agtcaacttt attcttaggg atatcgtgag actccggcag
     8941 tgcgtaagcc tgtaattttg gaatagccat cgtgttctct ccttcaggct gatgcgcgtg
     9001 acgccagcca ctgacgtaat tgttttttat cgactttccc gaccgccgtc agcggaagtg
     9061 aatctacaca ctccacgcga tccggtaatt taaattcggc aataccctgt tcacgcagga
     9121 aacgacgcac ctgcaccgcg cgcagcggct cttttaccac cagataagcg cagctttttt
     9181 cgcccatcag ctcatcttcc atgctcacca gtgcggcgta gatcaccgcc gggtggcgca
     9241 gcagcaggtt ttcgatctct tcggcagcga tcttctcacc gccacggttg atctgatctt
     9301 tctcgcgccc ctgcacggtg atgtacccct ctggatcaat agagatcaga tcgccggaac
     9361 agtaaaaacc gttggcatca aaggcgctgg cattgtgctg cgggcttttg taatagccac
     9421 ggaaggtgta cggaccgcgg gtcatcaggc gtccaacttc cccttgcggc agtggatttc
     9481 cttcggcatc ggcaacccac acttcatcgt ccggacacat tgggtaaccc tgggtatgga
     9541 taattttctc cgcgctatca tcaagacggg tgtagttcac cagcccttcc gccatgccaa
     9601 acacctgctg caactggcag ccaatctcag cgggaatacg cgccgcaagg gtggcagaaa
     9661 gacgtgcgcc gccgacctgt aacagtttca gcgaggcaag ctgcgcccgg ctttcacctt
     9721 cggtcagcgc ctgcaaccac aggctgactg ccggtggcac cagcgcggtg acgttcacct
     9781 gatgtttttc aatcaatggg aagcaaagcg tggcgctggg atcggccgcc agaacaaccg
     9841 ttcctccggc aagaaagacg cccagcgatc ccggcgaact catggcgtag ttatgagccg
     9901 ccgggatcgc gcacaggtag cgtgtctgtt gtgtgaactg acaaatctcg acgctacgac
     9961 gcacgctgta gtagtagtcg ttatgagtgc gcgggatcag tttcggtgtg ccggtggtgc
    10021 cgccggaaag ctggaaatag gccacttcat cagcaggtga tggcgtggca gtaaaatcct
    10081 cagccggatg gttaatcgca tcctgcaagt tatgctcacc gctgtcgttg tgcagttgca
    10141 ccacgcgaat ggaggaatgt tctgtgacga aagtattgag gaaatcatcc ccgctaaaca
    10201 gcgcatgttg gcgatcggca atcagcaatg cgggttcaat ctggctggca taggcgttca
    10261 gttcactacg ctgatggctg aacagcgcca gcaccggcgc aacgcccagt ttcagcagcg
    10321 cgaaaaaggt aatatacaat tcagcgacgt tacccagttg taccagcgcg gtttcaccag
    10381 gtttaatgcc ctgacggcgt aaactacacg cgaggttatc cgccgcctga ttcagctccc
    10441 ggtaactcaa ctgtcgctcg ccgtcgataa ccgcgatgct gtcactcgca gcgtggcgag
    10501 tcagaatgtc ggtcagcggc aaatcctgcc agtagccttt ttcccgatag cgacgggcaa
    10561 actcttccgg ccagcgggtg aatggaatgc tcatcctcgc tccttaatgc aatccaaaaa
    10621 cgttcaacat ggtagaaagt ttgacgcctg tttcgcgcca ctcacccaac ggtgacgacg
    10681 caggcacaat ccccgctccg gcaaacagac gcacctgatt ttcccgcagc ttcgcgcagc
    10741 ggatggtcac cacccattcg ccgttacctt cgctgtcaca ccaacccaca atgccgccaa
    10801 acagttcgcg gtcgaatggc tccagttcag caataacctg ggtcgcggcc tgatgcggga
    10861 aaccgctcag cgcgggggtt ggatgcagca gacaggccag agtcagtgcg ttttcttgcg
    10921 aattcgcttt accttcaaag ggagttgcga gatgccacag cgtcggcgtg gtaatcaatt
    10981 gtggagagga gggaacgtgt aactcactac tgcgttcgcg cagtacctct ttcatcgcct
    11041 gagtcaccag ttcatgttca tggcgatctt tttctgacgc cagcagacga ttacccgctt
    11101 cgcgatcgag cacttcatcc ggctgacgac gcgcggaacc ggctaacgga atggagctaa
    11161 aacgctcgcc gtctttacgt agcagcagtt ccgggctggc ccccagcagg acgccaccat
    11221 cagccagcgg gacatggaag ttgtaactaa ccgggttttg cgcaatcaac cgttccagca
    11281 atacgccact atcaatggcg gcgtcagtgg tgatatcaat caatcgtgac aacaccactt
    11341 tgtcgacctg cggcgtggcg gtaagtgcgg cggcgcgggc aaccatctgt tcaaacgtgg
    11401 tttgttcagg aattgcctgg cgttccacca cgttcagcga ctggctgcgg gtaaaacggc
    11461 gggctgaggt ctgtttttcc tggcgggaga acgactgcca ggattcggga atatacagcg
    11521 acgaaggctg acgtggatcg aagggaatcg ccccgaccat caccggattt ttgatgccct
    11581 gcgctttggc atcggcaaac agcgcggcga gtttttgctg gaagggactg tcgggcgaat
    11641 ccccgttcac agccggttca tcgaagcggg cgaaacatcc tgacgtcgta aaactgcggt
    11701 acggcgacat aaagaaaaag cgattgggcg caagtgttgc catggtctgc tgtacttcct
    11761 cagccagtga cgtatccata tcatcctcca caaaatgata aaggctttaa taatgattat
    11821 catttatatt ttcggtcgct aacctaaaag caaacgcgca ctatgtcaac tcttgaggta
    11881 acgcaatctg caactcgggg ttgcatctgg ttgtggttac aatgaaaatg agaagcatta
    11941 ttgatggatt cgcataagcg caatgtgatg tcctgcgccg ttctgccccc tctcccttcc
    12001 agggtgaggg ctggggtgag ggttaatgtt cgcaccagtg ctggctgttc ccctcaccct
    12061 ctccccaaag gggcgagggg acggattgtg cgctttgtcg aatttgtcat tacgcccata
    12121 accttattaa taacaggaag ctgatttgtg agactcgccc cgctctaccg caacgccctt
    12181 ctattaacag gacttttgct ttcaggaata gccgcagttc aggccgctga ctggccgcgt
    12241 cagattactg acagccgtgg cacacataca ctggaaagcc agccgcagcg tattgtttcc
    12301 accagcgtca ccctgaccgg ctcactgctg gcgattgatg ctccggtgat cgccagcggc
    12361 gcgaccacgc cgaataaccg cgtcgcggat gaccagggct ttttacgcca gtggagcaag
    12421 gtggcgaaag aacgcaaact gcaacggctc tatatcggcg aaccgagcac cgaagccgtt
    12481 gccgcgcaaa tgccggatct gattttaatt agcgcaaccg gcggggattc ggcgctggca
    12541 ctgtatgatc agctttccac catcgccccg acattaatca tcaattacga cgacaaaagc
    12601 tggcagtcgc tgttaacgca gcttggcgaa attaccgggc atgagaaaca agcggcagag
    12661 cggattgcac agtttgataa gcaactggca gcggcgaaag agcaaatcaa attaccgccg
    12721 cagccggtca ctgccattgt ctataccgcc gctgcacaca gtgccaatct ctggacgccg
    12781 gaatcagcac aagggcagat gctggaacaa ctcggcttta cgctggcgaa gttgcccgca
    12841 gggttaaacg ccagccaaag ccagggcaaa cgccatgaca tcattcagct tggtggggaa
    12901 aatctggctg ctgggttaaa tggcgagtca ctattcctgt tcgccggtga tcagaaagat
    12961 gccgatgcga tttatgccaa tccactgctc gcgcaccttc ccgcagtaca aaacaagcag
    13021 gtttatgcgc tgggaaccga gacgttccgt ctggattact acagcgccac gcaagtgctg
    13081 gaacgactta aggcgctgtt ttaagcatta actgtcagac gctgtcacct gcggcggcgt
    13141 ctggcgaaaa cgtcgcaact ccaccagcac cagcaataac aacacgccga taatcaacaa
    13201 accaaaaccg cttgcgcttg cggaagcaac cggtgtcatc atcgcgccca aaccacccag
    13261 cagcgccgcg cctatggcat cgcccgtcac gttctgcgcc gtccacaaac cgttaatccg
    13321 ccctaacatc gcttccgggg tttgcgtttg cagcattgta tattgcagca acgaactaac
    13381 cgcgctcagc cagccgaaca gcgccagaca aaccacgcct aaaatccaca tcggcatcag
    13441 gccaaacaga ccaatggcga ggaacgatcc cagcgtggag agcagcatca ataaccctgg
    13501 tcgcgcacta tgtgccagct tcccgctggt taacgcacca atagccgcgc cgagcgggat
    13561 cgccgcgtag agaaaaccaa tctgtgccgc tgacatctgc cagttgtcag ccagcgccgg
    13621 atacagtacc cgcaccgcgc tcgccatcgt caataaacca cccagcagcg caatcccgcc
    13681 caccagcggg ctggcaagca gaaaacgaaa tcctgccagt aatgatttca acggatgctc
    13741 acgcggctgc ggtggcggtg gcaacgccgg aaggcttaac aacggtagca aggtaataaa
    13801 cgtgcccgcc gccgccagcc cgtagttcca ggctacgcca ccggtcgcca gcaataaacc
    13861 gccaatcatg ggcgaaatca ccgaccccag acgcacggtc aacatggtga tcgccccggc
    13921 ctgcattaag ttttcacgcc ctaccagtgc tggtgtcgcc gccaatagcg ccgtaacgcc
    13981 aagcgatgcg aaaaaaccat cccataaacc aagtaaatag attgccagca atgacggctc
    14041 cggcagcagt gcattaaggc acagtccaat gaagccaatg ccacaggtgc cgcgcgccag
    14101 caaaatcact tttttgcgct catagcgatc cgccagcaca ccgccaacca tcaggccaac
    14161 aaacatcgcg ccgccggtca gcgtcaccga aagccccacc tgccaggtag aatgcgtcat
    14221 catctggatc tgcaccggca ccgcgacgcc gagcaaaccc agagacacaa ttgagataaa
    14281 acgagcgagg aatactgcgc gaaacgccgg gtgcgttttc aacaggctga ggttaagcag
    14341 ccaggattgt ttattcatta caatgccttg ccatcaaata ttttaaatac ctattcttag
    14401 gctgcacatg ctaacatatc caaataagat agataacgat aattaatttc attatcatgg
    14461 aagttcgtat gtctggttct gttgccgtga cacgcgccat tgccgtgccc ggattgctgt
    14521 tattactgat tatcgcgacg gcattaagcc tgctcattgg ggcaaaatca ctccccgctt
    14581 ccgtagtgct ggaggccctc tccggcacct gccagagcgc cgactgcacc atcgtgctcg
    14641 acgcgcgact gccgcgcacc cttgccggtt tacttgcagg cggcgcgctt ggccttgccg
    14701 gggcgttgat gcaaaccctc acccgaaatc cacttgccga ccccggcttg cttggcgtga
    14761 atgccggagc cagctttgcc attgtgctgg gtgcggcgct gtttggttac tcatccgcgc
    14821 aggaacaact ggcgatggcc ttcgccgggg cgctggtggc ctcattgatt gttgccttta
    14881 ccggcagtca gggcggcggg cagttaagtc cggtgcgttt aaccctggcg ggcgtggcgc
    14941 tggcggcggt gctggaaggg ctgaccagcg gcatcgccct gcttaatcct gatgtctacg
    15001 atcaactgcg tttctggcaa gccggttcgc tggatattcg caatctacat accttaaaag
    15061 tggtgctgat cccggtgctg atcgccggag caaccgcgct attactgagc cgcgcgctga
    15121 acagtttaag cctcggcagc gacaccgcga cggcgctggg cagtcgcgtg gcgcgcacac
    15181 agttaattgg tctgctggcg attaccgtgc tttgtggtag tgcgacggcg gtagttggcc
    15241 cgattgcctt tatcggcctg atgatgccgc acatggcgcg ctggctggta ggtgccgatc
    15301 atcgctggtc gctgcccgtc acgctacttg ctacccctgc cctgctgctg tttgccgata
    15361 tcatcgggcg ggtgattgtg cccggcgaac tgcgcgtttc tgtggtcagt gcgtttattg
    15421 gtgcaccggt gctgatcttc ctcgtgcgac gtaaaacgcg aggtggcgca tgatttacgt
    15481 ctctcgccga ttaatcatca cctgtttgct gttggtttcc gcctgtgtgg ttgcaggtat
    15541 ctggggatta cgcagcggtg ccgtcacgct ggaaacctcg caggtattcg ccgcgctgat
    15601 gggcgatgcg ccgcgcagta tgacgatggt ggtcaccgaa tggcgtttac cacgcgtgct
    15661 gatggcgctg ttgattggcg cggcactggg cgtcagcggc gcgatttttc agtcgctgat
    15721 gcgtaacccg ctcggcagcc ctgacgtaat gggctttaac accggggcgt ggagcggcgt
    15781 gctggtagcg atggtgctgt ttggtcagga cctgacggct atcgcgctgg cagcaatggt
    15841 gggcggcatt atcacttcgc tgttggtctg gctgctcgcc tggcgtaacg gcatcgacac
    15901 ctttcggttg attattatcg gtatcggcgt tcgtgccatg ctggtggcct ttaatacctg
    15961 gctgttgctg aaagcgtctt tagaaacggc gctaacagcg ggtttgtgga atgcaggatc
    16021 gctcaacggc ctgacgtggg caaaaacctc gccttccgca cccatcatta tattgatgct
    16081 cattgccgcc gccttactgg tacgccggat gcgcttgctg gaaatgggcg atgataccgc
    16141 gtgtgcgctg ggcgtcagcg tcgaacgttc gcgtctgtta atgatgctgg ttgcagtggt
    16201 gcttaccgct gcggcaaccg cgcttgccgg gccgatttcc tttattgctt tagtcgcgcc
    16261 gcacattgcc cgacgcatta gcggcaccgc tcgctggggg ctgacccagg cggcgctgtg
    16321 cggggcgctg ttactgctgg cggccgatct ctgcgcccaa caactgttta tgccgtatca
    16381 acttccggtt ggcgtcgtta ccgtcagcct cggcggtatt taccttatcg tcttgttaat
    16441 tcaggagtct cgcaaaaaat gaacgaatct gtagcccgtt tgcgcggcga acagttaacc
    16501 ctcggatatg gcaaatatac cgttgcggaa aatctgactg tagaaatacc tgatggtcac
    16561 ttcacggcaa ttatcgggcc aaatggctgc ggtaaatcca ctttactgcg tactttaagc
    16621 cgcctgatga cgcctgctca cgggcatgtc tggctggatg gcgagcacat tcaacattac
    16681 gccagtaaag aggttgcacg ccggattggt ctgttggcgc aaaatgctac cacgccaggc
    16741 gatatcaccg tgcaggagct ggtggcgcgt gggcgttacc cgcatcaacc gctatttacc
    16801 cgctggcgca aagaagatga agaagcggta acgaaagcga tgcaggccac gggaataact
    16861 catctggcag atcaaagcgt ggataccctt tctggcggac aacgccagcg cgcgtggatc
    16921 gcgatggtgc tggcccagga aacggcaatt atgctgctcg acgaacccac gacctggctg
    16981 gatatcagtc atcagattga tctactggaa ctgttaagcg aactgaaccg cgagaaaggc
    17041 tataccctgg cggcggtgct gcacgatctt aatcaggcct gtcgttacgc cagccatttg
    17101 attgcattgc gggaagggaa aattgttgct cagggagcgc cgaaggagat tgtcactgct
    17161 gaactgattg agcgcattta tggtctgcga tgcatgatca ttgacgatcc ggtagccgga
    17221 acgccgcttg tggtgccgct cggacgaacg gcaccttcaa ccgcaaatag ttaaactaag
    17281 tggtctgcca tcatggcatc ctgttttctg gatgccatcg catagcgcaa taacacgcta
    17341 ccacaagcca ccatcccgcc gatcagcgcg gaaaggatca caataatcgc cttacccgga
    17401 ccgtcttttt tcactggcaa tgacgggctt aactgatatt taaacggcgt aaaattcaca
    17461 tcgttgatat ttgcttttgt taactgctca acaagatact gacgattacg taattcaccg
    17521 ttcagttccg caacgtcagt gaccgctttt tcaatttcca gtttgcgttc aataccgtct
    17581 gcgccgagag aaatagagaa atcggggtca tctttaacgg cctgaccatt actgtatacg
    17641 ggctttttaa ttcccgccgc gttggcaatg tcgagtgaat aattgaggcg ctgaatgttt
    17701 gcatcaagtt gatttttcgt tttaatgcga tcctgagcca gtttttcttt ttcaaactgg
    17761 gttttgattt ccagtttatt acggacgttt tctagtgact ctttcaccac caacgcagag
    17821 atataatcga tatacccgct caaaacggtc tgcgcctctt cactggttgg cgcggtaaaa
    17881 cttagcgtcc aggaggtata cagtgacggt tcatcttttt tcttactggc attgtcatca
    17941 accgctttca ttttttcgct caatgcaaca atagcgcgat gcaaatccag ttcatcgatt
    18001 ttcgcctctt ttaattggtc catcacataa ggtgatgaac gcaggtactc ttccagcaag
    18061 ctaaccgact gaaacttctt gataaacagg ttaaatgctt ctgtacgatc aattttgata
    18121 tccagatcca gcactcgaag cttagtgaat gttttctcca gctcttgcca ctgaacaggt
    18181 tctggaggcg tgacaaccgc cgcgctggtc catttttgcg gcaggatgaa agagatcagc
    18241 aagcctgcgc aggcaaacgc aaaaatgacc gccatgaccg tttttttggc ccgccataaa
    18301 actgcgatta gattaagtaa atcaatttca ttattactgg gcgacgccag aggatactca
    18361 ggaaaatgag cgtcacttcc ctgtttaata ttcagtgatg acatggggta taacctgaaa
    18421 atgagtccaa taggggaaat ctccacggcg ctcactttac cagttggtga taattatccg
    18481 aagctgaagt ttgtaaattc cttccactga at
//
LOCUS       DAZGLJ010000080        18401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000080 DAZGLJ010000000
VERSION     DAZGLJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.080"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(19..642)
                     /gene="rsmG"
                     /locus_tag="ACHK49_002425"
     CDS             complement(19..642)
                     /gene="rsmG"
                     /locus_tag="ACHK49_002425"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO9263060.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGH
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(706..2595)
                     /gene="mnmG"
                     /locus_tag="ACHK49_002426"
     CDS             complement(706..2595)
                     /gene="mnmG"
                     /locus_tag="ACHK49_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO9263061.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2973..3416)
                     /gene="mioC"
                     /locus_tag="ACHK49_002427"
     CDS             complement(2973..3416)
                     /gene="mioC"
                     /locus_tag="ACHK49_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO9263062.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3506..3964)
                     /gene="asnC"
                     /locus_tag="ACHK49_002428"
     CDS             complement(3506..3964)
                     /gene="asnC"
                     /locus_tag="ACHK49_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO9263063.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4116..5108
                     /gene="asnA"
                     /locus_tag="ACHK49_002429"
     CDS             4116..5108
                     /gene="asnA"
                     /locus_tag="ACHK49_002429"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO9263064.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5113..6564)
                     /gene="viaA"
                     /locus_tag="ACHK49_002430"
     CDS             complement(5113..6564)
                     /gene="viaA"
                     /locus_tag="ACHK49_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO9263065.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6558..8054)
                     /gene="ravA"
                     /locus_tag="ACHK49_002431"
     CDS             complement(6558..8054)
                     /gene="ravA"
                     /locus_tag="ACHK49_002431"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO9263066.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8277..10145
                     /gene="kup"
                     /locus_tag="ACHK49_002432"
     CDS             8277..10145
                     /gene="kup"
                     /locus_tag="ACHK49_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO9263067.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDTVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10312..10731
                     /gene="rbsD"
                     /locus_tag="ACHK49_002433"
     CDS             10312..10731
                     /gene="rbsD"
                     /locus_tag="ACHK49_002433"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO9263068.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10739..12244
                     /gene="rbsA"
                     /locus_tag="ACHK49_002434"
     CDS             10739..12244
                     /gene="rbsA"
                     /locus_tag="ACHK49_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO9263069.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12249..13214
                     /gene="rbsC"
                     /locus_tag="ACHK49_002435"
     CDS             12249..13214
                     /gene="rbsC"
                     /locus_tag="ACHK49_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO9263070.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13239..14129
                     /gene="rbsB"
                     /locus_tag="ACHK49_002436"
     CDS             13239..14129
                     /gene="rbsB"
                     /locus_tag="ACHK49_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO9263071.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14255..15184
                     /gene="rbsK"
                     /locus_tag="ACHK49_002437"
     CDS             14255..15184
                     /gene="rbsK"
                     /locus_tag="ACHK49_002437"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO9263072.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15188..16180
                     /gene="rbsR"
                     /locus_tag="ACHK49_002438"
     CDS             15188..16180
                     /gene="rbsR"
                     /locus_tag="ACHK49_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO9263073.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16146..17573)
                     /gene="mdtD"
                     /locus_tag="ACHK49_002439"
     CDS             complement(16146..17573)
                     /gene="mdtD"
                     /locus_tag="ACHK49_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO9263074.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPVMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(17596..18288)
                     /gene="yieP"
                     /locus_tag="ACHK49_002440"
     CDS             complement(17596..18288)
                     /gene="yieP"
                     /locus_tag="ACHK49_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO9263075.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4432 a         4556 c         4798 g         4615 t
ORIGIN      
        1 tgattttttt gataaaaatt aaattttatt tgctttaatc accaccagat gacgttcgcc
       61 atccaaggct ggaacctgaa gtttaaccac tgattcgacc tgatattctt cgggcaacaa
      121 agcgatttca tcttccggca tttgcccttt cagcgcgtag aaatggcctt gctcaccagg
      181 aagatggtgg caccagctca ccatatcgtt cagagaggca aaagcgcggc taattacgcc
      241 atcaaatggc ggctctgaag gaaactcttc taccctgctc tgtactggtt caatattctc
      301 cagtttaagc tcatgttgca cctgacgaag gaaacgcacg cgtttaccaa ggctatccaa
      361 cagagtgaaa tgggcttcag gacgcacgat agagagtgga atgcctggca gtcctggtcc
      421 ggtgccgaca tcgataaacc gttcaccttg cagatacggt gccaccacaa tgctatcgag
      481 aatatggcgt accagcatct cattaggatc gcggaccgaa gtcaggttgt acgctttgtt
      541 ccatttatgc agcatattca cgtaggcaat aagctggttt ttctggtgat cggtaagcga
      601 aatacctgcg tctttcagca gtaaggagag tttgttgagc acggtgatta cctgttcttg
      661 atgcgttgcc tggtaagcgg gtgcttacca ggcattttta atgcgttatg cgctacgacg
      721 cagcataccc tgttttttca gccacaccag cagaatggag atggccgcag gcgtgacgcc
      781 agaaatacgc gaagcctggc cgatagaggc tggtttgtga tcgttaagtt tggcgatcac
      841 ttcgttagaa agaccggata cctggcggta atccagtgtc gcgggtagca gggtgttctc
      901 gttacgcagc tgcttttcga tctcatcttg ctggcgcgcg atataacctt cgtatttaac
      961 ctgaatctca acctgttccg ccgcctgttc gtctgtcaac gcaggggcaa acggcgtcag
     1021 cgtggttaat ttttcataag tcatttccgg acgacgcagc agatcttcac cactggcttc
     1081 acgggaaagc ggcgcagtca ggtgagcatt cacttcggct gcagcttccg ccgacggggt
     1141 tacccaggtc gatttcagac gctgacgctc acgctcgata ttctcaagtt tctcgttaaa
     1201 gcgcgcccaa cgttcgtcat ccaccaggcc cagttcacga ccgatttcag tcaaacgcag
     1261 atccgcatta tcttcgcgta gcatcagacg atattctgcg cgcgaagtaa acatacgata
     1321 cggttctttg gttcctaaag tgcacaggtc atcaactagt acgccgagat acgcctgaga
     1381 acgtgccgga gcccaacctt ctttgtcagc agacagacgg gcagcgttaa gaccggccag
     1441 caaaccttgc gcagcggctt cttcgtaacc ggtagtgccg ttaatctgac cagcaaagaa
     1501 cagcccctgg ataaacttgc tctccagcgt cggtttcagg tcgcgaggat cgaagaagtc
     1561 atactcaatg gcataacccg gacgcacgat cttcgcgttt tccatcccct gcatagagcg
     1621 gacgatttgc atctgcacat cgaacggcag gctggtggag ataccgttcg gataaatttc
     1681 attagaggtc agtccttccg gttcaaggaa gatctgatgc tgatttctgt cggcgaagcg
     1741 cataactttg tcttcgatcg acgggcagta gcgtgggccg acaccttcga tcacccctgc
     1801 atacattggg ctacgatcga ggttactgcg gatcacatca tgggttttct cgttggtatg
     1861 agtgatataa cacggcacct gctggggatg ctgggacgca ttgcccataa acgagaatac
     1921 cggcattggg ttatcgccat gctgttgcgc cagcacacta aagtcgatag ttcgagcatc
     1981 aatacgcggt ggtgtcccgg ttttcagacg accaacgcgc agcggcagtt cacgcaaacg
     2041 gcgagaaagc ggaatggacg gcggatcacc agcacggcca ccgctgtaat tatccagacc
     2101 gatatgaatt ttaccgtcga ggaacgtccc aacggtgagc acgacggctt tggcacggaa
     2161 cttcagtccc atttgggtaa cagcaccgac cacgcgatcg ttttcgacaa taagatcttc
     2221 aaccgcctgc tggaagatca tcaggttcgg ttggttctcc agcgccgtac gtaccgcctg
     2281 acggtagagc acacgatccg cctgagctcg ggtagcgcga accgccggtc ctttgcttgc
     2341 gtttagtatc ctaaactgga tacccgcttg atcgatcgct ttcgccatca gaccgccgag
     2401 tgcatccact tcttttacca gatgtccctt cccaataccg ccgatcgccg ggttgcagct
     2461 catctgcccc agagtgtcga tattgtgtgt caaaagcaga gtctgttgac ccatacgcgc
     2521 cgcggccatc gcggcctcgg tgcctgcatg acccccgcca atgatgatga cgtcaaaagg
     2581 atccggataa aacatggtga ttgcctcgca taacgcggta tgaaaatgga ttgaagcccg
     2641 ggccgtggat tctactcaac tttgtcggct tgagaaagac ctgggatcct gggtattaaa
     2701 aagaagatct atttatttag agatctgttc tattgtgatc tcttattagg atcgcactcc
     2761 ctgtggataa caaggatccg gcttttaaga tcaacaacct ggaaaggatc attaactgtg
     2821 aatgatcggt gatcctggac cgtataagct gggatcagaa tgaggggtta tacacaactc
     2881 aaaaactgaa caaccgttgt tccttggata actaccggtt gatccaagct tcctgacaga
     2941 gttatccaca gtagatcgca cgatctgtat acttatttga gtaaattaat ccacgatccc
     3001 agccattctt ctgccggatc ttccggaatg tcgtgatcaa gaatgttgat cttcagtgtt
     3061 tcgcctgtct gttttgcacc ggaatttttg agttctgcct cgagtttatc gatagcccca
     3121 caaaaggtgt catattcacg actaccaata ccgattgcgc caaagcggac cgcagaaaga
     3181 tcgggcttct gttcctgcaa tgcttcatag aaaggagaaa ggttgtccgg aatatctccg
     3241 gcaccgtggg tggagctgat aatcagccag atccccgagg cagataaatc ttccaacagc
     3301 ggaccgtgca gcgtttcggt ggtaaaaccc gcctcttcca gcttttcagc caggtgttct
     3361 gctacatatt cggcaccgcc gagggtgctg ccgctgataa gagtgatatc tgccataaac
     3421 cgccaccttt attaagagtg gcgtattgta cgctgtgaac gcgttgggat ctacctgtgg
     3481 aaaagtatgg gattaaaaaa gccgattatg gcttgatggt acgcatgatc gggttttgca
     3541 ggacgatcaa tgtctcggtg gactgaattt catcaattgt ttggatcttg ttgataagta
     3601 catgctggag agcgtcgatc gaacggcaca tcacttttat aaagatgctg tagtggccgg
     3661 ttgtgtaata ggcttcagtg acttcatcaa ggctttccag ctttgccagc gcggaagggt
     3721 agtctttggc gctctttaat ataatgccga taaagcagcc tacgtcataa ccgagctgct
     3781 tcgggctgac atcaatacgc gccccggtaa tgatccccgc ctgcttcatt ttctctactc
     3841 gaacgtgaat cgtccccgga ctgacgccaa attgtttcgc cagttcggcg taagcggtgc
     3901 gcgcattgcc cattaatgct tccaggatgc cacggtccag attgtcgatc agataatttt
     3961 ccataggatt ttcttatgca gattgatgat tcattctatt ttagccttct tttttaatga
     4021 atcaaaagtg tgttaggctt tttattgaat gattattgca tgtgtgtcgg tttttgttgc
     4081 ttaatcataa gcaacaggac gcaggagtat aaaaaatgaa aaccgcttac attgccaaac
     4141 aacgccaaat tagcttcgtg aaatctcact tttctcgcca actggaagaa cgtctggggc
     4201 tgatcgaagt ccaggcgccg attcttagcc gtgtggggga tggcacgcag gataacttgt
     4261 cgggctgtga aaaagcggtg caggtaaaag tgaaagctct gcctgatgcc cagttcgaag
     4321 tggttcattc actggcgaag tggaaacgtc agaccttagg gcaacacgac ttcagcgcgg
     4381 gcgaagggct gtacacgcac atgaaagccc ttcgccccga tgaagagcgt ctttctccgt
     4441 tgcactcggt ctatgttgac cagtgggact gggaacgcgt aatgggcgac ggtgagcgtc
     4501 aattctcgac tctgaaaagc acggtagagg cgatctgggc gggaattaaa gcaaccgaag
     4561 ctgcagttag cgaagagttt ggtttggcac cgttcctgcc ggatcagatc cacttcgtac
     4621 acagccagga gttactgtct cgttatccgg atctcgatgc caaagggcgt gagcgggcga
     4681 ttgcgaaaga tcttggcgcg gtattcctcg tcgggattgg cggcaagctg agtgatggtc
     4741 atcgtcacga cgtgcgcgca ccggattatg atgactggag caccccgtca gagctgggcc
     4801 atgcgggtct gaacggcgat attctggtat ggaaccctgt actggaagat gcgtttgagc
     4861 tttcttccat ggggatccgc gtggatgccg acacgttgaa gcatcagttg gcgctgaccg
     4921 gtgatgaaga tcgtctgcag ctggaatggc atcaggcgct gctgcgcggt gaaatgccgc
     4981 agaccatcgg cggcggtatc ggccagtctc gtttgaccat gctgctgctg caactgccgc
     5041 atatcggcca ggttcagtgt ggagtatggc cagctgctgt tcgcgagagc gtcccttctc
     5101 tgctgtaata atttatcgcc gccagcgtct gagcaggcgg cttcgcatcc cggtatcaaa
     5161 gcgccagata tgatcgaaaa tgcgcatgat gccgggtttg ccgtgtgccg acatcgccac
     5221 ggcatgaaag cgatgctgat gtacccgctg cagctctttc actttactcg tcacgtcgtc
     5281 aggcaaccgc tgagcgataa aatcagaaat caccaccgca tcggcatcaa accattccct
     5341 gctttgcaag cgttccataa tggcgcgaaa acaactggca agatcggtgc cgccacgaaa
     5401 ctgctggctt aaaaaacgga ttgcttgttc gatgccttgt gggcctgaaa gctcataacg
     5461 gacgatctcg gtggaaaata gcataatata gcagcgccgg ttttcagcga gagcaatgcg
     5521 catcaaggcc aggcagaacg ctttcgcaca ctgttcatta aagccgccca ttgagccgga
     5581 agtatccaca cagacaataa acggcccgcg cggctgttca tcgtaatctt tatgtaccac
     5641 cggacgttcg atcacttttt cacgccacga ctcaccgtgc aggcgatagg tgagcaactg
     5701 tttttccacc agccgacggt aaaactcata ctccagttcc gttatcccta gtgtcgccag
     5761 ttctggcggc agaagacgta aaatatcatc gctttgttgc agaccatcaa cctgctcagg
     5821 aaccgtcgcc ggttcgcgca ccatggtgcg gaaggtttcc atctgcgcat cgttgcgcgg
     5881 tattgatttg gcttcccgag aacgccccag ctgctctgcc aggcgtttca gttccggctg
     5941 ttcgttaaga aattcaccgt atttcacaat caactgatag tcgccacgtt taagctgacc
     6001 ggcgctcata tcccacagac gaccagctgc agtattgtta tctgcgagaa tcggttcaag
     6061 ttgtccgctc agcgtcatgc gttcctgaac ttcactcaac agttgttcgc gttcttcttc
     6121 taatagctgt tgatttaacg tcgttgcttg cacgatcaga cttaaacgcc aacgctggag
     6181 aaaaagcgtg tgtaacgctg aagtgatcgt gctgttagca tcaaccaact gtcgggcttg
     6241 ttctgcccat ggagaattca gacgatgcag taagtccagg atctgtggta gctgcacaat
     6301 aaactgtggc gtggagagga gctggctttg ctgatagcac atcacctctt cggtgagttc
     6361 cggcgggact cgggcatctt tcagccgact gcgcagcgct tcacgccagc ggggaacatc
     6421 atcagtgatt gccgccttca ggcgtgggaa tttttcaaag aagaccgcca gctgcggtga
     6481 ggccagcagt gcgatgatca tctcttcgat caatccctct tcgctgacgg ccagcatcac
     6541 attaagcgta tccagcgtta gcattgttgt gcctggcgaa tctgtgcacc gacatcctgt
     6601 aggctggctt caatgcgacc taaccagtcg ccagggataa acagacattt ttgctgttcg
     6661 ctgaataaag cgtgttgctt gcgccagtcg ctttcaagct cttccagttg ttgttttatt
     6721 tcaccaggca gaccttcagc cgatgaaccg ggaagtgcca gcgtactgcc ttgtaaactt
     6781 acatcgcgaa cgacaagatg ttgggcgcta tcaacttcca gattcagttt ctgggcaaag
     6841 ccgataccgt tcagtttacc gcgaatttca ccacctttgc tcagccactg ttccagcgcg
     6901 ctacgctcaa aggagatatg aaccacttcc atatcatgca gttttaacgg tttttgcagc
     6961 agtagagtca gagtggaagc agtaacgtta acaggaagtt gatactgctg acgacggctg
     7021 aaaatgccgc ccagacgaat taccgttaag gctgttttat cgctttgttg ctgctggagt
     7081 tgcaggtgac gttgcacaat cgcgccaagg cgagtcaaca tcccttgctg ttgccaggcg
     7141 tgaccggtca tcaatacatc aatttgttgt tgtatcaaat tcaggctttg cgcgtcatac
     7201 cacaggcaat ctttcagcaa aatgagatct accggggcaa cagcactgcg accgctaaaa
     7261 aaggcgctgg cctgcaataa tcggatcgct tttttccagc gacgatccga gacataaggc
     7321 gcatccggta atttatccag ttgctggcgc agcataaaaa tcagctcaaa tacatgatcg
     7381 ggcagcgtaa tttcaccaat ctctttctgc cagcgttcat attcttcatc tgtgacctgc
     7441 aaggcatcag gaaccggatt gtcgttttca tcctgttgac tggtcagcat ggagcggaaa
     7501 ttcgctttat cctgcacttt atctaaccac agacgaatca gcatgcggtc atataacgct
     7561 tccagactgc tgtctgcttc cggcagctcg ttggaggccg ccaccagcag acgcatcggg
     7621 attttttcta cgtgtgcacc gttgcggaac tggcgctcgt taatggcggt gagcaaggta
     7681 ttaagaattg ccgggcccgc tttccagatc tcatccagaa agacaatttc tgcttccggc
     7741 aggtaaccgc tggttaaacg ttcatagcgc ccttcatctt ttagcgcctg aatagaaagg
     7801 ggaccaaaaa cttcttccgg cgtggagaag cgggtcatca gatattcaaa cgcgcgggca
     7861 ttctgaaagg cgaattttaa gcgccgggcg atcaaacttt tggcaatacc tggcgggcca
     7921 aggaggaaca cactttcacc acttaatgcc gctaataaac acaagcggat ggcgtggcta
     7981 cgttcataaa gccccttttc caacgaactg ctcaggcggg aaattctttc cgctaataaa
     8041 tgagggtgag ccataatgaa gtggcgtcct ttcgtcaaaa gttctgcgta aattgcgagt
     8101 atagacgttt cttgctggtg gctaaaatag tctcaaaggg ggggtatttt tctttgagcc
     8161 aggttaatgt ggccgcattt aggagtacga ttttgccgtt aatcgtgcat actgtgcgct
     8221 tttttgtggg ccaagggact aagcacacat ttcatatttc aacgaaagac tagtctatga
     8281 gcactgataa taagcaatca ttgcccgcga ttaccctcgc ggcgattgga gttgtctacg
     8341 gcgatattgg taccagcccg ttatatacac ttcgtgaatg tttgtccggc cagtttggtt
     8401 ttggcgttga acgcgatacc gtgtttggct ttttatcgct gatcttctgg ctgctaatct
     8461 ttgtggtttc cattaaatat ctcaccttcg tgatgcgggc agataacgcc ggtgaggggg
     8521 ggatcctgac gttgatgtcg cttgccgggc gtaatacgtc ggcgcgaacc acatcaatgc
     8581 tggtgattat ggggctaatc ggcggcagct ttttctatgg tgaagtcgtc ataacacccg
     8641 ctatttcggt gatgtcagcc attgaaggtc tggaaatcgt cgccccgcag ctggatacct
     8701 ggatagttcc cctctcaatt atcgttctca ctttattatt tatgattcaa aaacatggca
     8761 cggctatggt cggtaagctg tttgcgccga tcatgctgac ctggtttttg attctggcag
     8821 gactggggtt acgtagcatt attgctaacc cggaagtgct gcatgcgctg aatccaatgt
     8881 gggcggtgca tttcttcctt gaatacaaaa cggtttcttt tattgcatta ggggcagtgg
     8941 tgttgtcgat tacgggggtc gaggcgctgt atgctgatat ggggcacttt ggtaagttcc
     9001 ctattcgcct ggcgtggttc accgtcgtat taccttcctt gacccttaat tacttcggtc
     9061 agggagcgct gttgttaaag aacccggaag cgattaagaa cccgttcttc ctgttggcac
     9121 cggactgggc gctgatcccg ctgctgatca tcgccgcact ggcgacggta attgcctcgc
     9181 aggcggttat ctctggcgtc ttctcattga cgcgtcaggc ggtacgtctg ggatatttgt
     9241 cgccgatgcg cattattcac acctccgaaa tggagtcagg gcaaatctat attccgtttg
     9301 tgaactggat gctctatgtc gcggtcgtga ttgtgattgt cagctttgag cactccagca
     9361 acctggcggc ggcgtacggg attgcggtga ccggaaccat ggtgctgacg tctattctct
     9421 cgactaccgt ggcacgtcag aactggcact ggaataagta ttttgttgcg ctgatcctga
     9481 ttgctttcct ttgtgtcgat attccattgt tcaccgctaa cctcgataaa ctgctctccg
     9541 gcggctggtt gccattgagc ctcggtactg tgatgtttat cgtgatgacc acctggaaga
     9601 gcgagcgttt ccgcttgctg cggcggatgc atgaacatgg taactctctg gaagcgatga
     9661 ttgcttcgct ggagaaatca ccgcctgttc gcgtgcccgg gaccgcggtg tatatgtcgc
     9721 gtgcaatcaa cgtcattccc tttgcgctga tgcataacct taaacataac aaggtattgc
     9781 atgagcgggt gattctgtta actctgcgta ccgaagacgc gccatatgtc cataatgtcc
     9841 gtcgggtaca gattgaacaa ctgtcgccca ctttctggcg cgtggtggca agttatggtt
     9901 ggcgagaaac gccaaacgtg gaagaagttt tccaccgctg cggtctggaa ggattaagtt
     9961 gccggatgat ggaaacctcc ttctttatgt cgcatgagtc gttgatcctc ggcaaacgcc
    10021 cgtggtattt gcgtctgcgc ggcaagctgt acttgctgct gcaacgtaat gcgctgcgtg
    10081 caccagatca atttgaaatt ccgccaaaca gggttatcga actgggtact caggtcgaaa
    10141 tctaacgcca gacgcctcct ttctccataa gggggcgttt ttgttttcat ggttaatcac
    10201 catgtaaaac gtttcgaggt tgatcacatt tccgtaacgt cacgatggtt ttcccaactc
    10261 agtcaggatt aaactgcggg tcagcgaaac gtttcgctga tggagaaaaa aatgaaaaaa
    10321 ggcaccgttc ttaattctga tatttcatcg gtgatctccc gtctgggaca taccgatacg
    10381 ctggtggtgt gtgatgctgg tttacctatc cccaaaagta caacgcgtat cgatatggca
    10441 ttaacccagg gtgtaccttc ttttatgcag gtgctgggcg tcgtcacaaa tgaaatgcag
    10501 gtcgaggctg ccattatcgc ggaagagatc aaacaacata atccgcaact ccacgaaacg
    10561 ttgctcactc accttgagca gctgcaaaaa caccagggaa ataccattga aattcgttac
    10621 accacgcatg aacaattcaa acaacaaacc gcagaaagtc aggcggtaat tcgcagcgga
    10681 gaatgttctc cgtatgcgaa tatcattctc tgtgctggcg tgacgttctg aggccgtcat
    10741 ggaagcatta cttcagctta aaggcatcga taaagccttc ccgggcgtaa aagccctctc
    10801 gggcgcagcg ttaaatgtct atccgggccg cgtgatggcg ctggtgggcg aaaacggcgc
    10861 gggtaaatcc accatgatga aagtgcttac tggtatctat gctcgcgatg ccggcacgct
    10921 tttatggctg gggaaagaaa cgacatttac cgggccgaaa tcttcccagg aagccgggat
    10981 tgggattatc catcaggaac tgaacctgat cccgcagttg accattgccg aaaacatttt
    11041 cctcggtcgt gagtttgtta atcgctttgg caaaattgac tggaaaacca tgtatgccga
    11101 agcggataaa ttgctggcta aacttaacct gcgctttaaa agcgacaagc tggtgggcga
    11161 tctttccatc ggtgaccagc aaatggttga aatcgccaaa gtgctgagct ttgagtcgaa
    11221 agtcatcatt atggatgaac cgaccgatgc gctgaccgat accgaaaccg aatccctgtt
    11281 ccgcgtcatc cgcgaactaa aatcgcaagg ccgcggtatt gtctatatct cccaccgcat
    11341 gaaagaaatc ttcgagattt gcgatgacgt taccgttttt cgtgacgggc aatttattgc
    11401 tgagcgcgaa gtggcatcac tgaccgaaga ttcgctgatt gagatgatgg tgggccgcaa
    11461 gctggaagat caatatccac acctggacaa agcgccggga gatatccgcc tgaaagtcga
    11521 taatctctgc ggacctggcg ttaacgatgt ctcttttact ttacgcaaag gcgaaattct
    11581 tggcgtctct ggtttgatgg gcgcaggtcg taccgaactg atgaaagtgc tctacggcgc
    11641 actaccgcgc accagcggtt acgtcaccct ggatgggcat gaagtcgtta cccgttcacc
    11701 gcaggatggc ctggcaaacg gcattgtgta tatctccgaa gaccgtaaac gtgacggttt
    11761 agtgttgggc atgtcagtaa aagagaacat gtcgctgaca gcgctgcgct acttcagccg
    11821 cgctggcggc agtttgaagc atgccgatga acagcaggct gtgagtgatt tcattcgtct
    11881 gtttaatgtg aaaacaccgt cgatggaaca ggcaattggt ctgctttccg gtggcaatca
    11941 gcaaaaagtg gcgattgccc gcggtctgat gacacgcccc aaagtgttga tccttgatga
    12001 acctacccgt ggcgtagatg tcggcgcgaa aaaagagatc tatcaactga ttaaccagtt
    12061 caaagccgat ggcttgagca tcattctggt gtcatcggag atgccagaag tattaggcat
    12121 gagcgatcgc atcatcgtca tgcatgaagg gcatctcagc ggggaattta ctcgtgagca
    12181 ggccacccag gaagtgttaa tggctgccgc tgtgggcaag cttaatcgcg tgaatcagga
    12241 gtaaaaaaat gacaacccag actgtctctg gtcgccgtta tttcacgaaa gcgtggctga
    12301 tggagcagaa atcgcttatc gctctgctgg tgctgatcgc gattgtctcg acgttaagcc
    12361 cgaacttttt caccatcaat aacttattca atattctcca gcaaacctcg gtgaacgcca
    12421 ttatggcggt cgggatgacg ctggtgatcc tgacgtcggg catcgacttg tctgtaggtt
    12481 ctctgttggc gctaaccggc gcagttgctg catctatcgt cggcattgaa gtcaatgcgc
    12541 tggtggctgt cgctgccgct ctcgcgttag gtgccgcaat tggtgcggta accggggtga
    12601 ttgtagcgaa aggtcgcgtt caggcgttta tcgctacgct ggttatgatg cttttactgc
    12661 gcggcgtgac catggtttat accaacggta gcccagtgaa taccggcttt actgagaacg
    12721 ccgatctgtt tggctggttt ggtattggtc gtccgctggg cgtaccgacg ccagtctgga
    12781 tcatggggat tgtcttcctc gcggcctggt acatgctgca tcacacgcgt ctggggcgtt
    12841 acatctacgc gctgggcggc aacgaagcgg caacgcgtct ttctggtatc aacgtcaata
    12901 aaatcaaaat catcgtctat tctctttgtg gtctgctggc atcgctggcc gggatcattg
    12961 aagtggcgcg tctctcctcc gcacaaccca cggcggggac tggctatgag ctggatgcta
    13021 ttgctgcggt ggttctgggc ggtacgagtc tggcgggcgg aaaaggtcgc attgttggga
    13081 cgttgatcgg cgcattaatt cttggcttcc ttaataatgg attgaatttg ttaggtgttt
    13141 cctcctatta ccagatgatc gtcaaagcgg tggtgatttt gctggcggtg ctggtagaca
    13201 acaaaaagca gtaataacga ctacaggaca tcttgaatat gaacatgaaa aaactggcta
    13261 ccctggtttc cgctgttgcg ctaagcgcca ccgtcagtgc gaatgcgatg gcaaaagaca
    13321 ccatcgcgct ggtggtctcc acgcttaaca acccgttctt tgtatcgctg aaagatggcg
    13381 cgcagaaaga ggcggataag cttggctata acctggtggt gctggactcc cagaacaacc
    13441 cggcgaaaga gctggcgaac gtgcaggact taaccgttcg cggcaccaaa attctgctga
    13501 ttaacccgac cgactccgac gcagtaggta atgctgtgaa gatggctaac caggcgaaca
    13561 tcccggttat cactcttgac cgccaggcaa cgaaaggtga agtggtgagc cacattgctt
    13621 ctgataacgt actgggtggc aaaatcgccg gtgattacat cgcgaagaaa gcgggtgaag
    13681 gtgccaaagt tatcgagctg caaggcattg ctggtacatc cgcagcccgt gaacgtggcg
    13741 aaggcttcca gcaggccgtt gctgctcaca agtttaatgt tcttgccagc cagccagcag
    13801 attttgatcg cactaaaggt ttgaacgtaa tgcagaacct gttgaccgct catccggatg
    13861 ttcaggctgt attcgcgcag aatgatgaaa tggcgctggg cgcgctgcgc gcactgcaaa
    13921 ctgccggtaa atcggatgtg atggtcgtcg gatttgacgg tacaccggat ggcgaaaaag
    13981 cggtgaatga tggcaaacta gcagcgacta tcgctcagct acccgatcag attggcgcga
    14041 aaggcgtcga aaccgcagat aaagtgctga aaggcgagaa agttcaggct aagtatccgg
    14101 ttgatctgaa actggttgtt aagcagtagt tttaatcagg ttgtatgacc tgatggtgac
    14161 ataaatacgt catcgacaga tgaacgtgta atataaagaa aagcagggca cgcgccaccc
    14221 taacacggtg gcgcatttta tggacatccc gaatatgcaa aacgcaggca gcctcgttgt
    14281 tcttggcagc attaatgctg accacattct taatcttcaa tcttttccta ctccaggcga
    14341 aaccgtaacc ggtaaccact atcaggttgc atttggcggc aaaggcgcga atcaggctgt
    14401 ggctgctggg cgtagcggtg cgaatatcgc gtttattgcc tgtacgggtg atgacagcat
    14461 tggtgagagc gttcgccagc agctcgccac tgataacatt gatattactc cggtcagcgt
    14521 gatcaaaggc gaatcaacag gtgtggcgct gatttttgtt aatggcgaag gtgagaatgt
    14581 catcggtatt catgccggcg ctaatgctgc cctttccccg gcgctggtgg aagcgcaacg
    14641 tgagcgtatt gccaacgcgt cagcattatt aatgcagctg gaatcaccac tcgaaagtgt
    14701 gatggcagcg gcgaaaatcg cccatcaaaa taagactatc gttgcgctta acccggctcc
    14761 ggctcgcgaa cttcctgacg aactgctggc gctggtggac attattacgc caaacgaaac
    14821 ggaagcagaa aagctcaccg gtattcgtgt tgaaaatgat gaagatgcag cgaaggcggc
    14881 gcaggtactg catgaaaaag gtatccgtac tgtactgatt actttaggaa gtcgtggtgt
    14941 atgggctagc gtgaatggtg aaggtcagcg cgttcctgga ttccgggtgc aggctgtcga
    15001 taccattgct gccggagata cctttaacgg cgcgttaatc acggcgttgc tggaagaaaa
    15061 accattgcca gaggcgattc ggtttgccca tgctgccgct gcgattgccg taacacgtaa
    15121 aggcgcacaa ccttccgtac cgtggcgtga agagatcgac gcatttttag acaggcagag
    15181 gtgacgcttg gctacaatga aagatgttgc ccgcctggcg ggcgtttcta cctcaacagt
    15241 ttctcacgtt atcaataaag atcgcttcgt cagtgaagcg attaccgcca aagttgaagc
    15301 ggcgattaaa gaactcaatt acgcgccatc agccctggcg cgtagcctca aactcaatca
    15361 aacacatacc attggcatgt tgatcactgc cagtaccaat cctttctatt cagaactggt
    15421 gcgtggcgtt gaacgcagct gcttcgaacg cggttatagt ctcgtccttt gcaataccga
    15481 aggagatgaa cagcggatga atcgcaatct ggaaacgctg atgcaaaaac gcgttgatgg
    15541 cttgctgtta ctgtgcaccg aaacgcatca accttcgcgt gaaatcatgc aacgttatcc
    15601 gacagtgcct actgtgatga tggactgggc tccgttcgat ggcgacagcg atcttattca
    15661 ggataactcg ttgctgggcg gagacttagc aacgcaatat ctgatcgata aaggtcatac
    15721 ccgtatcgcc tgtattaccg gtccgctgga taaaactccg gcgcgcctgc ggttggaagg
    15781 ttatcgggcg gcgatgaaac gtgcgggtct caacattcct gatggctatg aagtcactgg
    15841 tgattttgaa tttaacggcg ggtttgacgc catgcgccaa ctgttatcac atccgctgcg
    15901 tcctcaggcc gtctttaccg gaaatgacgc tatggctgtt ggcgtttacc aggcgttata
    15961 tcaggcagag ttacaggttc cgcaggatat cgcggtgatt ggctatgacg atatcgaact
    16021 ggcaagcttt atgacgccac cattaaccac tatccaccaa ccgaaagatg aactggggga
    16081 gctggcgatt gatgtactca tccatcggat aacccagccg acccttcagc aacaacgatt
    16141 acaacttact ccgattctga tggaacgcgg ttcggcttag atttacgctg tcttttgatc
    16201 aaattattac catcggttgt tttcagaagc atgaacattg ctgctgaagc aacagtaata
    16261 atgcccatcg tgataaacgt atagtggaat tgttcgacag tcgttgtgcc ttccatccct
    16321 tcataaacgc gaaggacggc cgcacttaca gcaacgccta aactaatcga cagttgctgc
    16381 gtgaccgcca gaacactgtt accgctgctg gcgttgtcat cggtcagatc ggcaagtgtg
    16441 atggtattca tcgcggtaaa ttgcgtcgac atagccatcc ctaatataaa caacggcaag
    16501 atcagcatcc atatcgccat taccggtgat tgcaaagaga actgagcgat cattagccca
    16561 ataatcaccg tgatccccac taacgtatgg cgatagccca gacgacgtaa gacttgggta
    16621 accatcgatt ttgcaataat ggaacctaac gctgtcggtg ccatcataca gccggcaata
    16681 aacgcctgat aaccaaatcc tacctgtaac atcaatggca taaggaacgg tacacaaccg
    16741 gtccccagac gggttgcaat attgcctacg ataccgatcg agaaagtgcg ggttttaaat
    16801 aaatctaatg aaattaatgg gtttggcgtg tgtcgcgcat ggagaatata gagaagcagt
    16861 aacccgatgc tggtgacaat taccgtcaag gcaatccagc tggcgacaat cttttccccg
    16921 aatagctcta ttccgcttga gaagagaaca aggctgaggc caaacagcaa aaagccagtg
    16981 atatcgaatc tgcgtcgtgc ggtggtgaaa ttgggcatat gtttgcgcgc gtaaagaagg
    17041 cccgcaatac ctatggggat attgattaaa aatatccagt gccaggttgc ccaggtgacc
    17101 agcacgccgc caagaacggg gcctaaaatt ggccccacca gacccggcat ggcgacaaaa
    17161 ttcaatactg gaagaagttc attacgagga taagcgcgca gtaaggccag ccgagcaaca
    17221 ggcatcatca ttgcgccgcc tatcccctga ataacccgga agacaaccag ctgtggtagc
    17281 gaattagaaa gtgcgcaggc cagagaaccc aatgtgaaca gactcacggc aagggtaaaa
    17341 atgcgacgcg taccgaagcg atcggctagc catccgctta ccggaataag catcgccacc
    17401 gtcagcgtat aactgatgat ggctgattgc atcgcgagag gagaacgatt aaggctatga
    17461 gcgattgcgg gtaaggcggt attcagaata gtggcatcaa gtgcctgcat gaagaaggcc
    17521 atcgccgcga tccacggcaa acccgccata ctgcgcttct ttttatcgct cattcaatgt
    17581 cctattatcg ggttgtcact tatcaggtga gcgtagcagc gcctgacaag ctttaaatgc
    17641 cgcgtcgcca tcgctttgga caatcgcatc gacaatcgcc tggtgcagat ccagctttat
    17701 cactgtgtcg ctggtaattg acgtgaagta agtgtgataa accgaatgga atagcgaggc
    17761 gaatgaggtc aaaaacggat tggcgctcat ttcatagata tgctcatgcc aggccatatc
    17821 gacttcgatc cagcgttcac ggcgaaagtt ctcttttaat gccgccattt cggccattaa
    17881 cgtattgaga tgcgccttct gttccgcggt gccaaccgtt gcagccagta ggcaagcttg
    17941 cggctccaga cagatacgca taaccagaaa gtgatcgatg acctgatgaa agttctcttc
    18001 tgtcatccac caggtaagca attcctgatc aagaaaattc cagtttgatt gtggcatgac
    18061 ccgggtacca attcgcggtc gcggtaaaac catccctttt gccgttaacg ttttgaccgc
    18121 ttcgcgtacc gctgtacgac tcactccaaa ttgctcgccc agctcaatct caccaggcaa
    18181 aatggtgccg ggttcatatt caccttttaa gatgcgttgc gccagcttct cagccagaac
    18241 atacgaaagg tttttctgtg cagctaactg ttgtgcgctt aaaggcatta ctaatcttcc
    18301 tttttctttt tattcctcct tagtatgcca ccaggaagtg tgattacggt tgcaaaaacg
    18361 gcaaattgct tgttttatgg cacattaacg gggcttttgc t
//
LOCUS       DAZGLJ010000081        18300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000081 DAZGLJ010000000
VERSION     DAZGLJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.081"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..942
                     /locus_tag="ACHK49_002441"
     CDS             <1..942
                     /locus_tag="ACHK49_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFO9263076.1"
                     /translation="LFLFSLPLIIFYQPWVNALPPMPRHANPEQLEKTVRYLTQTVHP
                     RSADNIDNLNRSAEYIKEVFVSSGARITSQDVPITGGPYKNIVADYGPADGPLIIIGA
                     HYDSASSYENDELTYTPGADDNASGVAGLLELARLLHQQVPKTGVQLVAYASEEPPFF
                     RSDEMGSAVHAASLERPVKLMIALEMIGYYDSAPGSQDYPYPAMSWLYPDRGDFIAVV
                     GRMQDINAVRQVKATLLSSRYLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAVMITDTA
                     FYRNKQYHLPGDTADRLNYQKMAQVVDGVINLLYNSK"
     gene            1045..1548
                     /locus_tag="ACHK49_002442"
     CDS             1045..1548
                     /locus_tag="ACHK49_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO9263077.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1615..3072)
                     /gene="nuoN"
                     /locus_tag="ACHK49_002443"
     CDS             complement(1615..3072)
                     /gene="nuoN"
                     /locus_tag="ACHK49_002443"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO9263078.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3079..4608)
                     /gene="nuoM"
                     /locus_tag="ACHK49_002444"
     CDS             complement(3079..4608)
                     /gene="nuoM"
                     /locus_tag="ACHK49_002444"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO9263079.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFDISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSRSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4772..6613)
                     /gene="nuoL"
                     /locus_tag="ACHK49_002445"
     CDS             complement(4772..6613)
                     /gene="nuoL"
                     /locus_tag="ACHK49_002445"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO9263080.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLVT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6610..6912)
                     /gene="nuoK"
                     /locus_tag="ACHK49_002446"
     CDS             complement(6610..6912)
                     /gene="nuoK"
                     /locus_tag="ACHK49_002446"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO9263081.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6909..7463)
                     /gene="nuoJ"
                     /locus_tag="ACHK49_002447"
     CDS             complement(6909..7463)
                     /gene="nuoJ"
                     /locus_tag="ACHK49_002447"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO9263082.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7475..8017)
                     /gene="nuoI"
                     /locus_tag="ACHK49_002448"
     CDS             complement(7475..8017)
                     /gene="nuoI"
                     /locus_tag="ACHK49_002448"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO9263083.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8032..9009)
                     /gene="nuoH"
                     /locus_tag="ACHK49_002449"
     CDS             complement(8032..9009)
                     /gene="nuoH"
                     /locus_tag="ACHK49_002449"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO9263084.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9006..11732)
                     /gene="nuoG"
                     /locus_tag="ACHK49_002450"
     CDS             complement(9006..11732)
                     /gene="nuoG"
                     /locus_tag="ACHK49_002450"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO9263085.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGA
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11785..13122)
                     /gene="nuoF"
                     /locus_tag="ACHK49_002451"
     CDS             complement(11785..13122)
                     /gene="nuoF"
                     /locus_tag="ACHK49_002451"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO9263086.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13119..13619)
                     /gene="nuoE"
                     /locus_tag="ACHK49_002452"
     CDS             complement(13119..13619)
                     /gene="nuoE"
                     /locus_tag="ACHK49_002452"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO9263087.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13622..15424)
                     /gene="nuoC"
                     /locus_tag="ACHK49_002453"
     CDS             complement(13622..15424)
                     /gene="nuoC"
                     /locus_tag="ACHK49_002453"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO9263088.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15518..16180)
                     /gene="nuoB"
                     /locus_tag="ACHK49_002454"
     CDS             complement(15518..16180)
                     /gene="nuoB"
                     /locus_tag="ACHK49_002454"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO9263089.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16196..16639)
                     /gene="nuoA"
                     /locus_tag="ACHK49_002455"
     CDS             complement(16196..16639)
                     /gene="nuoA"
                     /locus_tag="ACHK49_002455"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO9263090.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17270..18208)
                     /gene="lrhA"
                     /locus_tag="ACHK49_002456"
     CDS             complement(17270..18208)
                     /gene="lrhA"
                     /locus_tag="ACHK49_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO9263091.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4554 a         5098 c         4738 g         3910 t
ORIGIN      
        1 ttatttctgt tttcactccc tcttattatt ttttaccaac cctgggtcaa tgcattaccg
       61 ccaatgcccc gacacgcaaa tcctgagcaa ttagaaaaaa cagtgcgtta tcttacacaa
      121 actgtgcatc cacgtagtgc cgacaatatt gataatctga ataggtccgc agagtatata
      181 aaagaggtct tcgtcagtag cggtgccaga attacctcgc aggacgtccc cattacgggt
      241 ggcccctaca aaaacattgt tgctgattat ggtcctgccg atggaccgct gattattatt
      301 ggtgcgcact atgactctgc cagcagttat gaaaacgatg aattgaccta tacacctggc
      361 gcggatgata acgccagcgg cgtggcagga ttactcgaac tggcacgttt gttacatcag
      421 caagtaccga aaacaggcgt gcagctggtc gcctatgcgt cggaagaacc gcccttcttt
      481 cgtagcgatg aaatggggag cgcggtacat gcagcttcgc ttgagcgtcc agtaaaatta
      541 atgatagcac tggagatgat tggctattac gactctgcgc ctggtagcca ggattaccct
      601 tacccggcaa tgtcctggct ttatcccgat cggggagact ttattgccgt ggtcggcaga
      661 atgcaggata tcaacgccgt tcgtcaggta aaagcgacat tgttatcatc ccggtattta
      721 tctgtttatt ctatgaatgc cccagggttt attcccggta ttgatttctc tgatcacctt
      781 aattattggc aacacgatat tcccgccgta atgattactg acaccgcttt ttatcgtaat
      841 aaacaatacc acttgcccgg tgataccgca gacagattga attatcagaa aatggctcag
      901 gtggtggatg gtgttataaa tttgttatac aacagtaaat aataaaaatt tctcgaggcg
      961 ggtccagaaa aagcgacatg cacattttat tttccctcgt ctgttttaag atgcgaaaca
     1021 tgaaattcaa tggaggaaaa ataaatggga atgattggtt attttgcaga aattgactca
     1081 gaaaagatta accaactgct tgagtctact gaaaaacctt tgatggataa tatacatgac
     1141 acgctttcag gattacgccg actggatatt gataaacgct gggatttttt acactttggg
     1201 ctgacaggca cctctgcttt tgatcccgcg aagaatgatc cgctgagtcg tgccgtgctt
     1261 ggtgaacaca gcctcgaaga tggcatagat ggcttcctgg gattgacctg gaatcaggag
     1321 ttagccgcga caatcgaccg actggaatca ctggatcgaa gtgagctgcg taaacagttt
     1381 tccattaagc gactcaatga aatggagatt tatccgggag tgaccttttc agaagagtta
     1441 gaggggcaac tctttgcttc gatcatgctt gatatggaaa agctgattag cgcgtatcga
     1501 cgtatgctgc gacaagggaa tcatgcgctg acggttattg ttgggtgatg gtgtcagggc
     1561 accaaaaaaa accgccgaac atgtcggcgg ctttctgact tacaaactaa cagattacat
     1621 cagcggcatt gctaaccgca caatgctaat cagcggttgt ggccatacac ccagcaccag
     1681 taccaacagt gcagaaatca gcaccacgat accgcccgcg ctgtactgcc agtttgatgg
     1741 tgcatcgcga cccggttgtt ccggggcgtg aagatacagg ctcaccgcca cgcgcaggta
     1801 gtagtagagg ccgattgccg aaccgacaac cacggcaccc accagccacc acaagtgtgc
     1861 ctggacaccg accgccagca cgtagaactt accgataaag cccagcgtca tcgggatacc
     1921 ggccagagac agcatcatca ccgtcatcac tgccgcgagg atcggacgat gccagaacag
     1981 accgcggtaa gagaacaggg aatcagcatc cgggccacga tacgggctgg acatcaggct
     2041 gaccacgccg aacgcgccga ggctgctgaa cagataaccg gccaggtaaa cccctaccgc
     2101 ttccatcgac atctcaccag tttgcagcgc aatcagcgcc accagcagat agccgaggtg
     2161 agagatagat gagtaaccga gcagacgttt gatgttggtc tggctcagcg ccatcaggtt
     2221 accgaagatg atggaggcaa aggcgataat cgccagtact acgcgaatcg cttcgctgtc
     2281 acccaccggt gcgtagagga acagacgcat caccacaccg aagatagcga ttttgctcgc
     2341 cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg tatacgtctg gcgtccacag
     2401 gtggaacggc accagcgaga gtttgaagcc gaggccaaca atcatcagtc cgaaacctgc
     2461 cagcaacagc ggctcgttga gcataccgtc gccgaggttt ttacccagcg cgacaaacga
     2521 caggtcgcca gactgtgcat acaccagcgc cataccaaac agcaggaaag aagacgctgc
     2581 ggcagaaagg atggtgtatt tgatactggc ttccagcgaa cgtttctggc ggaaagcgta
     2641 accgaccagg ccaaacagcg gcaaagagat cagttcaata ccgaggaaca aagacgccag
     2701 atggttggca ttcgccagca ggatcccgcc cagcgcggca attaacacca acaggtagaa
     2761 ctcatccttg ttgtcgttat agccttcaag ccacgggtag gcgaaagtac aggtggcgag
     2821 gctcgccaac aataccagcc cggtgtaaag catggcgaaa ccatcaacgc gcatcagcgg
     2881 cgtaacgtcc atagcgcccg cctggccgac aaaccagagc gaaaccagcg ccgcgttaag
     2941 cccaataacc gagagcgtag cgttgaggaa atgattgcgt cgccacgcaa tggagagcat
     3001 cacaaccacc accgtcaagc cgacgatcag caacggtagc agtgcgatca ggttttgtgg
     3061 agttattgtc atggcgattt acggccttgt agtagtaacg gaattaacaa accactgctg
     3121 gatattgcca atcgcggagc gcgaggtatc cagaatcggc tgcggataga agcccagcag
     3181 taccagcagc accaccagca acaggatcat aaacagctca cgcagcgaca tccctggcag
     3241 ttcctggctg gcaatctggc ttttcgcttt accgaagtaa gcgcgatgca acatcgccag
     3301 cgaataaaca gatgcaaaga ccagcccaaa ggtagagata acggtaatca ccgggacaac
     3361 ctggaagctg ccgaacagaa tcataaattc gccgacgaag ttaccggtgc caggcatccc
     3421 aagcgttgcc accgcaaaga acagcgacag tgctggcagc catttcatct tgctccacag
     3481 accgcccatc atgcgcatgt cgcgggtatg gatacgttca taaagctgac cacaaagaat
     3541 aaacagaccc gccgccgaca agccgtgcgc aatcatctgg attaccgcgc cctggtaggc
     3601 caactggctg ccggtgtaga tagcaatcag cacgaagccc atgtgggaaa ccgaggtgta
     3661 ggcgatcaga cgtttgatat cggtctgggc gaaggccatc cacgcaccgt agaagatgcc
     3721 gataacaccc agccacatag caattggcgc gaactctgcc gacgcgttcg ggaacagcgg
     3781 cagggagaaa cgcagcaaac cgtaagcggc agttttcagc aagatccccg cgaggtcaac
     3841 ggaaccggcg gtcggagcct gggagtgcgc atccggcagc cagccatgca gcggaaccac
     3901 cggcattttg actgcgaagg cgatgaagaa gcccagcatt aacaggtatt ccacaccact
     3961 ggacattggc gtattcagca gctcttcata gttgaaggtc cagacgccgg tcgcattgta
     4021 gtgaacaaaa accagcgcca ggatggcaat caacatcacc aggccactcg cctgggtgta
     4081 aatgaagaac ttggttgccg ccgtgatacg cgttttaccg tcagaggctt tatgccccca
     4141 cagtgcgatc aggaagtaca tcggcaccag catcatttcc cagaagaaga agaacaggaa
     4201 catgtcgatg gcaaggaaca cgccgataac gccgcccagg atccacatca ggttgaggtg
     4261 gaagaagccc tgatattttt cgatctcttt ccacgaacat agtaccgcca gcacaccgag
     4321 cagaccggtc agcacgacca tcagcagcga cagcccgtca atggcgagat gaatagagat
     4381 atcaaaacgc gggatccacg gcatgtcgaa ttcagactgc cactgcggaa ttccggcgga
     4441 ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc gacagcgcca gagtcaatcc
     4501 catggtgatc agcgcgatcc agcgtggcac cttgacgcca aagcgttcgg tctgccagca
     4561 caggaagccg ccaataaagg gaattaatat tagccagggt agtaacatgg cgatctttat
     4621 tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat tctcacgaca gaaaattctc
     4681 ttcggactgg gggtagtgtc ggatgcaccg cgaaggcggc ttatccgacc tacgttctta
     4741 tatgcccagg ggcaatccca caatccttaa ctcaacgcag taccatcaac agtgccagca
     4801 ccacgaccgc accgatgctc atggatgcca cataccagcg cagatagccg ttctcactta
     4861 acagcagacc tttacctgca aagcgggaaa ggacagccgg gatgttcatc attgagttca
     4921 gcggatcgcg tttcagcaac caggcaatac ccaggaacgg cttgacgaac actttgtcat
     4981 acagccagtc aaatccccag gcgttgtacc accaggtgcc cagcagacgg cccggcgcac
     5041 tgttggcgat ggaggtcacc agagtacgtt tacccagcca cagccaggct gccagcagaa
     5101 tgccgaccac cgcgaccacg ccagaggtaa tttccagggt caacatgctg ccgtgcgcca
     5161 gttccgtcgt ttgcggaagc acgccctgca gcggcggtac aatcagtgcg ccaacgaagg
     5221 tggaaaggat cagcagcaca atcagcggca ggctgtgagt tacccctttc acggcgtgag
     5281 cgtgaatttg ttcttttccg tggaagacga tgaaaatcat acggaaggtg tagagcgagg
     5341 tcataaacgc accgaccaga cctgccacca tcagattgat atgaccattc gccatcgcac
     5401 ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac cagcggtagt gccgacagtg
     5461 ctgcgccgcc caccaggaag cagagataaa ccagcggaat agatttacgc agaccgccca
     5521 tcttgaagat gttctgttcg tgatggcagg ccagaatgac ggaaccggat gccaggaaca
     5581 gcagcgcttt aaagaacgcg tgagtcatca agtggaaaat cgccgcatcc catgcctgca
     5641 cgccaagcgc gaggaacatg tagccaatct ggctcatggt agagtaagcg agaacacgtt
     5701 tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag cagcgtaacc gccccgacaa
     5761 tacccaccag atgcagaact tccggcgtca ccaggaacag gccgtgggta cgggcgatca
     5821 ggtagacacc cgcggttacc atcgttgcgg cgtggatcag cgcggagaca ggcgtcgggc
     5881 ccgccatcgc gtcagcaagc catgtctgca acggcaactg cgcagattta ccgaccgcac
     5941 cgcccagcag catcagcgtc gcccacatca gcatgttatt gccgtcagca aagtgcgctg
     6001 gtgccagttc caccatttcg cggaagttca gggtgcccag ttcgttgtaa agaatgaaca
     6061 gtgcgaaagc gaggaaaacg tcacccacac gggtcacgac gaacgctttc attgccgctg
     6121 cgccattctt cggatcggtg taatagaaac cgatcagcag ataggagcac aggcccacgc
     6181 cttcccagcc gaggtacatc agcagcaggt tatcggcgag aaccagaact accatgctgg
     6241 cgatgaacag gttggtgtag gcgaagaagc gagagtagcc ctcttcaccg cgcatgtacc
     6301 aggaggcgta catgtgaata aggaaaccca caccagtgac caccgagagc atggtcagcg
     6361 acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc gcctaccgac atccacgtcc
     6421 acagcggctg gctgtatgac tgctcgccgt tagcgaagaa atcaacgccg ataaaggcgg
     6481 ttaccagcgc cgccaggccc acagagccaa cgccgacgat cgccgaaacg ttttcagacc
     6541 agcgcccacg ggagaacgcc agcaggacga agccaatcaa tggcaaaata atggttaagg
     6601 caagcatgtt catccgcgca tctcacttac tgaatcgatg ttcaggttct ggcgacgacg
     6661 gtgaagttgc agcagcagcg caaggccgat actcgcttct gccgccgcga ggctgatggc
     6721 gagaatgtac atcacctgac cgtcggtctg accccagtag cttccggcga ccacaaaggc
     6781 cagcgcggag gcgttaatca tgatttccag accaatcagc ataaacagca gattgcgacg
     6841 gataaccaga ccggttaagc caagaacgaa taagattgcc gcgaggatca gtccatgttg
     6901 taaggggatc atgcgtgctc ctccgttttt cttttcgcgc tgtcgtcttt acgattgctc
     6961 agcacttcac ccgcacgctc ttcacgaccg acgtggaagg ccacaaccag acccgcgagc
     7021 agcagcatag aagccagttc caccgccagt acgtaaggcc cgaacagcgt aatacccact
     7081 gctttagcac tgattggcgt accgtcgata ccctgatcat taacaccaag gatggcgtaa
     7141 acaatcacca ccagcatgat ggccgacaaa attgccggac caatccacac ctgcggtttc
     7201 agccactggc gttcctgttc gatttctgaa ccgcccaggt tgagcatcat caccacgaac
     7261 acgaacagca ccataatggc acccgcgtag acgataattt ccagcgcacc ggcgaagtaa
     7321 gcgcccagtg agaagaacac cccggaaatc gccagcagcg aaataatcag gtacagcagt
     7381 gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg caagtatggc tatcaggcca
     7441 cagatataaa aagcgaactc cattgcccct ctccttacgg taacaggctc ttgacgtcga
     7501 taggcttggc ttcgttctct gcttcgccct tatctttgcc gtcgattgcc atacctgcca
     7561 tccggtagaa gttatattcc gggtatttgc ccggaccgga gatcagcaga tcctcttttt
     7621 cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa atccggggtt aactgaatcg
     7681 ccgtggtcgg acaggcttct tcgcacagac cacagaaaat gcagcgtgag aagttgatgc
     7741 ggaaaaattc cgggtaccag cgaccgtctt tggtttctgc tttttgcagc gagatacagc
     7801 cgaccgggca ggctaccgcg cagaggttac aggctacgca acgctcttcg ccgtccgggt
     7861 cgcgggtcag aacgatacga ccacgataac ggggcggcag atagaccggc tcttccgggt
     7921 acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat catccagata ctacgaacct
     7981 gggtgccgaa acctactaac aattctttta aggtcatggt cttattgccc cttattgcgc
     8041 ctgccagaga atgacagccg ccgttaccag caagttgatc agcgtcagcg gcaggcagat
     8101 tttccagccg aaggacatta cctggtcata acgcggacgc ggtaacgacg cacgaatcaa
     8161 aatgaacatc atcataaaga acgcggtttt cagcgcgaac cagatgaatg gcggtaacaa
     8221 cgggccttgc cagccaccga agaacagcgt caccatcaat gcagagatgg tcacaatccc
     8281 gatgtactca cccacgaaga acagaccgaa cttcataccg gaatattcaa tgtggtaacc
     8341 atccgccagt tcctgctcgg cttccggctg gtcaaacggg tgacggtgac ataccgccac
     8401 gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg ataacgttcc acacatgcgc
     8461 ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc tgcgccacca cgcccatcag
     8521 ggaaagcccg aggaacactt cgtagctcag ggtctgcgca gaagcacgca tcgcacccag
     8581 caacgagtat ttgttgttac ttgaccagcc cgcaaacagc accgcgtaaa ccgccagacc
     8641 tgccatcatc aggaagaaca aaatcccgat gttcaggtcg gcaaccaccc aacccggact
     8701 gactggcaca atcgcaaagg ccagcagcag cgaggtaaag gcaatcatcg gtgccagggt
     8761 aaagatgacg cgatccgaga atttcgggat ccagtcttct ttaaagaaca ttttgatcat
     8821 gtccgcaacc agctggagcg aaccgcccca gccaacacgg ttaggtccgt aacggttctg
     8881 gaacagaccc agcaggcgac gttcgccaaa gctcatgaat gccccgcagg tgacaaccac
     8941 cagcaggatc accaccgctt tgaggatggt cagcaggatc tcaatcagtt ccggtgatat
     9001 ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg cgccagccag caccggagca
     9061 atgccggaca tacccatcgg caaacccacc tgccctgccg tcagcccttc agcgatttca
     9121 accggcagcg tgaccgtgtt gccatcgtaa ctaaaggaga cgcgagcgcc tgcgttcacg
     9181 cccaacttcg cggcatccgc tgggttgagt ttgatgtacg gctgcggcat acggctctgg
     9241 aagaccggag cacgctgtga caattcatcg ctgccaaaca ggtggtaata cggcgcgata
     9301 cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc tggtgaagta atccagacca
     9361 ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa agcgcagttt gccgcccact
     9421 tcgtcctgga atttgttcca cgcctgcggg gagttccagc ccggcgccca ggcaaacggc
     9481 acttgcgaac ggtgcgcagt cggctggttg ttaccttcca tcgagaaggt gaacatggtg
     9541 tcaatatcct gcggctgacg cggctcatga acgctgatat tggcgcgcat ggcggtacga
     9601 ccgctgtaac ggtgcggttc acgggccagt ttctgcccac gaatacggaa tgtcgcatcc
     9661 ggcgcagcat ctttgatacc tgccagttcc gggattttcg ccacaacagc gtcaatcaca
     9721 tggtcgagct gcgtccagtc cacttcacgg ctcagcaggg tgctgtgcag cgagtgtaac
     9781 cagcgccagc tttccagcat gacagttttg ctgtcgtaat aagcaggatc gtaaacctgg
     9841 aagaaacgtt gggcgcggcc ttcgttgttg atcaccgtac cgtcgctttc agcaaagctg
     9901 gcagcagaaa gtaccagatg ggcgttttcc ataatcgctg tgcgttgatg atcaaccacc
     9961 atcaccagcg gcgctttagc cagcgcagca ttcacgcggg tagcggaagc gtgacgatgc
    10021 agatcgtttt ccagcaccac caccgcgtcg gcgcgtccgg tttccagttc ggtcaacgct
    10081 tcttcaagcg aaccgccacc cataatgccc agccccatgc tgttgacgga acgggcaatc
    10141 atggtgatac caacgtcagc gccgcgacct ttcagggctt tcgcgacgtt agccgcagcc
    10201 tgaatcacct ctatgctacc ggcgttcgtc ccggagataa tcaacggttt cttcgcacct
    10261 gccagtgcct gcacgatgac gtcgattttg ctttgcagct caggttcgat accgtcaacc
    10321 gctggcgcag agttatccag cgcatgggcg atggcaaaac ctaaacgcgc ctgatcttca
    10381 accggtgcgc ggtaagtcca cgccgcgata tcatccagac gggtgtcatc aacgttggta
    10441 acaaacagcg gatgcttcgc acgttgaccg atgttgagga ttgccgcaat ctgccagtca
    10501 gccactttct gtgctgccgc catttcgcgc gctttacctt tcacagcctg acgcactgcc
    10561 agcgcgacgc gcgcgccggt ctgggtaacg tcttcgccca gcaccagtac cgcatcgtaa
    10621 gattcgattt cgcgcagagc cggagtataa atgccgcctt cacgcagcac tttcagcgcc
    10681 agttgcagac gttcctgctc accgtgagcg ataccggtgt agaagttttc ttcgcccacc
    10741 agttcacgca gcgcaaagtt gctttccacg ctggcacgcg gagaaccaat accgatcact
    10801 ttcttcgact gacgcagaat atctgccgcg ccctgcattg cctgttcggc gttgagggta
    10861 atgaaatcat cgccacgacg ctgtactggc tgacgcggac gatccttcag gttgacgtaa
    10921 ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt ttaccgtacc gttgtaacgg
    10981 ttttcgatac gacgcagttc gccgtaacgt tcaccggggc tgatgttaca gccgatggaa
    11041 cattgctggc agatgctcgg cgcaaactgc atatcccatt tacggttgta acgctcggag
    11101 tgcgttttgt cggtaaatac gccggtcggg caaatttcga ccaggttacc ggaaaattcg
    11161 ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt tgtcgtgcgc accgtaaacg
    11221 cccagatctg taccgtcagc gtaatctttg tagtaacgca cacagcggta gcaggcgatg
    11281 cagcggttca tttcgtgaga gatgaatggc cccaaatcct gattacggtg ggtacgtttg
    11341 gtgaaacggt agcgacggaa gctgtgtccg gtcatcacgg tcatatcctg aagatggcag
    11401 ttaccgcctt cttcacatac cggacagtcg tgcgggtggt tggtcattaa ccactcgacc
    11461 acactttcac ggaactgttt cgcttcttcg tcgtcaatgg aaataaaggt gccatcggaa
    11521 gccggtgtca tacaggacat caccaggcga ccacgcgtgt cttccgcgtt ttggtattgc
    11581 ttcaccgcac actggcggca agcaccgacg cttcccagcg ccggatgcca gcaaaagtaa
    11641 ggaatatcaa ggcccagaga cagacaagct tccagcaggt tgtccgctcc gttgacctcg
    11701 tattctttgc cgtctacatg aattgtagcc attagcatgc ttccagtttt ctcagtcaga
    11761 gactgagcgt taatcgaaat tcggttacca gcgctctttc agcaggttcg gctgaatccc
    11821 attaatcaaa tgggtattgc tgaacggctg tttgattccc gcctcaaatt cttcgcggaa
    11881 atatttgatg gcgctctgta acggctccac tgcaccaggt gcgtgggcac agaaagtttt
    11941 acccgggcct aagaatcgac acagttgctc aagtgtttcg atatcgcccg gctgaccttc
    12001 accacgctcc agcgcacgca gaattttcac gctccacggc agaccgtcgc ggcacggcgt
    12061 acaccagccg caggattcac gggcgaaaaa ctcttccagg ttacgcacca gcgacaccat
    12121 gttgatctca tggtcaaccg ccatcgccag cgccgtaccc agacggcttc ctgctttacc
    12181 gatactttcg aattccatcg gcaggtcaag atgcgcttcg gtcaggaagt cggtgcccgc
    12241 gccgcctggc tgccaggctt tgaatttcag accgtcacgc ataccaccgg cgtaatcttc
    12301 gaggatctcg cgcgcggtag taccaaacgg cagttcccac aggcccggat ttttcacccg
    12361 accggagaag cccatcagct tggtgccagc atctttactt ttcgagatgt tctgatacca
    12421 ctccacgccg ttagcgagga tcgccggaac gttacacagg gtttcgacgt tgttgacaca
    12481 ggtcggttta ccccatgcgc cggaggtcgc cgggaatgga ggcttcgagc gtgggttagc
    12541 acgacgtcct tccagggagt tgattaacgc tgtttcttcc ccgcagatgt agcgccctgc
    12601 cccggtatgg acgaacagtt cgaagtcaaa acctgttccc ataatatttt tgccaagcag
    12661 acctgcttcg gtggcttcgg caatggcacg gcgtagattc actgccgctt cgatatattc
    12721 gccacgcagg aagatgtagc cacggtaagc tttcagcgca aacgcggaga tgagcatacc
    12781 ttccaccagc aggtgcggca gttgctccat caacaggcgg tctttatagg tgcccggctc
    12841 catttcatcg gcgttacaca gcaggtaacg gatgttcatg gattcgtctt tcggcatcag
    12901 gctccacttc aggccagtcg aaaagcccgc gccgccgcgc cctttcagac cagcgtcttt
    12961 tacctgatta acgatttcgt ccggagacag cccggtcagc gccttacgcg ccccttcgta
    13021 accatttttg ctgcggtatt cgtccagcca cactggctgt ttgtcatcgc gcagacgcca
    13081 ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc atttataccg ctccagcagt
    13141 tcagggatcg cttccggggt cagatgcgcg tgagtgtcct catcgatcat catatttggc
    13201 cctttatcac agttccccag gcagcaggtt ggcagcagcg taaagcgacc gtcaaacgtc
    13261 gtttgccctg gtttgatgtt cagttttttc tccagcgccg cctgaatacc ctggtaaccg
    13321 ttgatgtgac agaccacgct gtcgcaataa cggatcacat ggcgaccaac cggttggcgg
    13381 aagatctggc tatagaacgt tgccacgcct tcgacatcgc tcgccggaat acccagcaca
    13441 tcggcgatcg cgtggatcgc gccgtccggc acccagccac gctgcttctg aacgattttc
    13501 agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt gcatctcgtg ctcaatcgct
    13561 tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg gttgttgatt ctcgtgcata
    13621 attagcggtc cacatctgac ataacaaaat cgatactgcc cagataaaca atcaggtcag
    13681 acaccaggct gccgcggatc gccgccggaa tttgctgcaa atgcgcataa ctcggcgtgc
    13741 ggatacgagt gcgataactc atggtgctac cgtcgctggt caggtagtaa ctgttgatcc
    13801 ccttggtcgc ctcaatcatc tggaaagatt cattggcagg catcaccggc ccccacgaca
    13861 cttgcaggaa gtgggtgatc agggtttcga tatgttgcag cgtgcgctct ttcggcggcg
    13921 gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg catgttgttg aggcactgct
    13981 caagaatgcg cagactctgg cgcagctctt ccactttcag cattacgcgg gtgtagcagt
    14041 cagaaacacc accaccaacc gggatttcaa agtcgaagtt ttcatagcca gaataaggac
    14101 gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag gcccgcgcca gtggtgcccc
    14161 actccagcgc ctctttcgcg ccataggcgg caacgccctg ggaacgacct ttcagaatgg
    14221 tattttgcag cgccgctttc tcgtaagacg ccagacgttt cggcatccag tcgaggaact
    14281 cacgcagcag gcgatcccag ccgcgcggca ggtcgtgcgc tacgccgcca atacggaacc
    14341 acgccgggtg catacggaaa cccgtgatcg cttccaccag atcgtaaatt ttctgacgat
    14401 cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc ctgaataaag gtcgagatgt
    14461 acagcaggtg gctgttgata cggaacagtt cggagagcat aacgcgaatg acgttaacgc
    14521 gatccggcac ggtgatcccg gccagtttct ctaccgccag cacgtaaggc atttcgttaa
    14581 cgcagccgcc gaggtattca atacggtcgg tatacggaat gtagctgtgc caggactggc
    14641 gctcgcccat tttctccgca ccacggtggt ggtagccgat gtctggtacg cagtcgacaa
    14701 tctcttcgcc gtcgagttgt aaaacgatac ggaaagcccc gtgcgccgac gggtggttcg
    14761 gaccgaggtt gaggaacatg aagtcctcgt tttcggtgcc gcgcttcatc ccccactctt
    14821 ccggtttgaa ggtcagggct tccatctcca gatcctgttt ggctttggtc agctcaaacg
    14881 gcgagaattc ggtagcgcgc gccgggtaat ctttacgcag cgggtgacct ttccaggttt
    14941 gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa agtaatgcca aacagatccc
    15001 aggtttcacg ctcataccag ttagcgttcg ggaacagttt ggtgaaggtc ggtacgtgca
    15061 ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg gttacgatcg atagaaatca
    15121 gatggtagaa aacggaaaaa tccgcggcag gtaacccttc gcggtgtgtg cgcagacgtt
    15181 cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt cggcagtttc tttaagaaat
    15241 cgccaacttc cagtaattgt tcacgcttga tccacacaac gggaaccccg gtgcgagtcg
    15301 cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag ttcgccaatt accggatcat
    15361 caagatgatc gcgggtctgc caggcgggtt cttgcgcggt taagtcggtc atattgttca
    15421 ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc ttcgcgaaat agatttagga
    15481 ataagcgaag aaaaatccct ttgccgacag gcgcaaatta aatctcgtca ggtgtacgca
    15541 ggttggttac ggcaatacgt tcaccgcgct tgcgttcgcg ctctgattgc atattggcgc
    15601 gataaacgcc ctgatcgcca accacccagg agagcggacg acgttctttg ccgatagatt
    15661 cctgcaacag catcagtgcc tgcatgtacg cttcaggacg cggcgggcag cccgggatat
    15721 acacatcaac cgggatgaat ttatcgacgc cctgcacaac ggaataaata tcgtacatac
    15781 caccagagtt ggcacaggca cccattgaga taacccattt tggttccagc atctggtcat
    15841 acagacgctg aataaccggt gccattttgg taaagcaggt tcctgcaacc accatcaggt
    15901 cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa acgcgccacg tcatgcaccg
    15961 cggtaaacga agtcaccatc tcaacgtaac agcaggaaag accgaagtta tacggccaaa
    16021 ttgagttttt acgaccccag ttaaccatgt cattgagctt gcccataaac acgtttttgt
    16081 taacttcttg ctccagaggg tcggttacga tctcctgctt ttgcaggggg taacggtcgt
    16141 tctcaccgtt gggatctatg cgggtgagcg tataatccat cttaatgcct cgcggttagc
    16201 gttgacgatt agcgatactg ttcgtttccg ggttcatacg ctcgcggcgt gaacgcgcgg
    16261 gcgtccagtc cagcgcgcca atacgcacca gataaaccag acctgccagt aacacaaaaa
    16321 taaaaattgc agcttccaca aagcctaccc agccgctttc gcggatagag gttgaccatg
    16381 cgaacagata cagcgcttca acgtcgaaga taacgaagaa catggccacc agataaaact
    16441 tggcggacag gcgtaagcgg gcggagccga ccgagtcgat accggattca aacggcacgt
    16501 ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc gcctaccagc atcaggcaac
    16561 acaggccaat ggcaacgata agaaagatag cgaatgccca gtgatgagcg atgacttcag
    16621 tggatgttga catactcatt gcttactcat caaaagtagc gccagattca ctgctcttca
    16681 cggcagatgg acgccacatc gattcatggg gaggaataaa aaaaacctta caatcactgt
    16741 agaaattctt ttatacagct aattgatgtg gtcttttact cctttctata accttttgtc
    16801 aactttaaca aaagtttctt cacattagtt tacataatat caacaccatt agcatttaat
    16861 gccctttcac cccagatcct tgacgactcc aggataatta gatgttgttg aatcgtgtcc
    16921 gttgtgaagc aatggaaaaa atacgggtct attttgacag gaatttgtgt cgattcctcc
    16981 ccccaaaaga gagtattttc ttgatctgtg acacgctttt gtcattccat aacaaaaacg
    17041 cagcaacaaa tttacgtatt ttttaacatc attgtagcag gtgatttttt tcaggcgatt
    17101 atttgtgcgt tcgggacgtg aatctctggt gattgaaaaa tgaacagttt tgtacgttct
    17161 gcactatgcg aggaaggatt tttactaaaa aaaagccgct ggggtttaaa acacccccag
    17221 cggctcgttt tttacactat tgtctcagga attatctatc gtccgtcgat tactcgatat
    17281 ccctttcaat caacaatgaa tcatcccctt ccggagcaga cattgggctg tattgccacg
    17341 gattatggta gctctccatc gcctgataga tcacctgggc cagctcatta ttactggacg
    17401 ggtcatagca cagcaaatat tcggtatcag gtagcggcgg taagccatct actccactca
    17461 gcacacgcag gtccgggctc atcatctcaa ccggccttgc cgtcacgcca agaccggctt
    17521 tcactgccgc acgaacggcc ggaagcgtcg aggcgacata agccagtcgc catggaatat
    17581 ctgctttatt aagcgtcgcc agcaccatat cgcgaaacgg acttggatca tccagcaata
    17641 caagagggat cggctcgcct ttttgcaata cgtattccgc tgcgcagtac cagtgtgttg
    17701 gcgaggttcg caggttcaat gccttaaacg ctgaaggacg atgggtggtt accatcaaat
    17761 ccacttcctg ggattccagc atttctgcca tataggcatt acgtttaacg cggacatcca
    17821 gcgctaattt cggataaacc gaactcacgc gatttaacag gaaaggtaag atcgtatcgg
    17881 cagattcatc tgaagcaccg atagttaaca cgccctgaag attactgaac attaatgatg
    17941 agcaggcctc atcattaaaa cgcaggattt tcctggcgta accaagaagt tgaatgccat
    18001 gttcagttaa cagtttgttg cgaccgtgac gagcgaacag ttctttccca acgagttgtt
    18061 ccagacgctg catttgctga cttacggcgg actgagtacg acacacagcg gcagctgcgg
    18121 cagcaaaagt gttcagatcg gcaacagcaa caaatgttct cagcagatcg aggtcgaggt
    18181 taattatcgg acgatttgca cttatcatat attatcactt actggcggct catactgagc
    18241 tggttaatgc tgtgcacaca caaacaagca attccatttg taatgtgcct ccctggcagt
//
LOCUS       DAZGLJ010000082        18297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000082 DAZGLJ010000000
VERSION     DAZGLJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.082"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            238..804
                     /gene="mepS"
                     /locus_tag="ACHK49_002457"
     CDS             238..804
                     /gene="mepS"
                     /locus_tag="ACHK49_002457"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO9263092.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            985..2541
                     /locus_tag="ACHK49_002458"
     CDS             985..2541
                     /locus_tag="ACHK49_002458"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO9263093.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            2623..4437
                     /locus_tag="ACHK49_002459"
     CDS             2623..4437
                     /locus_tag="ACHK49_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO9263094.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDTFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            4438..5532
                     /locus_tag="ACHK49_002460"
     CDS             4438..5532
                     /locus_tag="ACHK49_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO9263095.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSKYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            5532..6557
                     /locus_tag="ACHK49_002461"
     CDS             5532..6557
                     /locus_tag="ACHK49_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9263096.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            6559..8148
                     /gene="yejF"
                     /locus_tag="ACHK49_002462"
     CDS             6559..8148
                     /gene="yejF"
                     /locus_tag="ACHK49_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO9263097.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(8152..8496)
                     /locus_tag="ACHK49_002463"
     CDS             complement(8152..8496)
                     /locus_tag="ACHK49_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO9263098.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(8829..10019)
                     /gene="bcr"
                     /locus_tag="ACHK49_002464"
     CDS             complement(8829..10019)
                     /gene="bcr"
                     /locus_tag="ACHK49_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO9263099.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            complement(10047..10742)
                     /gene="rsuA"
                     /locus_tag="ACHK49_002465"
     CDS             complement(10047..10742)
                     /gene="rsuA"
                     /locus_tag="ACHK49_002465"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO9263100.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            10891..12651
                     /locus_tag="ACHK49_002466"
     CDS             10891..12651
                     /locus_tag="ACHK49_002466"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO9263101.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGNEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            12776..13060
                     /gene="rplY"
                     /locus_tag="ACHK49_002467"
     CDS             12776..13060
                     /gene="rplY"
                     /locus_tag="ACHK49_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO9263102.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(13199..14206)
                     /gene="yejK"
                     /locus_tag="ACHK49_002468"
     CDS             complement(13199..14206)
                     /gene="yejK"
                     /locus_tag="ACHK49_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO9263103.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            14388..14615
                     /locus_tag="ACHK49_002469"
     CDS             14388..14615
                     /locus_tag="ACHK49_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO9263104.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            14635..16395
                     /gene="yejM"
                     /locus_tag="ACHK49_002470"
     CDS             14635..16395
                     /gene="yejM"
                     /locus_tag="ACHK49_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO9263105.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            16470..16546
                     /locus_tag="ACHK49_002471"
     tRNA            16470..16546
                     /locus_tag="ACHK49_002471"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16504..16506,aa:Pro,seq:ggg)
     gene            16765..18006
                     /locus_tag="ACHK49_002472"
     CDS             16765..18006
                     /locus_tag="ACHK49_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO9263106.1"
                     /translation="MARKTHPLTTVQIKAARPAQKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLEDVSLADARKRRSEYRTLISAGTDPQDHERQKRETEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            18105..>18297
                     /locus_tag="ACHK49_002473"
     CDS             18105..>18297
                     /locus_tag="ACHK49_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263107.1"
                     /translation="MQKNIRCNCDGLQLALMVQHEFWSTYDPEDRTTAPSKKQVVDFL
                     VSRGASRNLAVSIDKVVRPA"
BASE COUNT         4540 a         4698 c         4595 g         4464 t
ORIGIN      
        1 cactttttct tataaaaaat catgtaaaac cgctcgccaa gaccgcacca atcgggtaat
       61 ctcgaactcg ttttgcctcg gcggtagatt atcctcacag catataattt tgtgcgttag
      121 tccacagatt tggccttaag gaattgtttc aacatgccca ggtaattagt ctcgtgtcgc
      181 ttggcatttt tttataacga tatttgtcgt taaggacttc aagggaaaac aaacaacatg
      241 gtcaaatctc aaccgatttt gagatatatc ttgcgcggga tccccgcgat tgcagtagcg
      301 gttctgcttt ctgcatgtag tgcaaataac accgcaaaga atatgcatcc tgagacacgt
      361 gcagtgggta gtgaaacatc atcactgcaa gcttctcagg atgaatttga aaacctggtt
      421 cgtaatgtcg acgtaaaatc gcgaattatg gatcagtatg ctgactggaa aggcgtacgt
      481 tatcgtctgg gcggcagcac taaaaaaggt atcgattgtt ctggttttgt acaacgtaca
      541 ttccgtgagc aatttggctt agaacttccg cgttcgactt acgaacagca ggaaatgggt
      601 aaatctgttt cccgcagtaa tttgcgtacg ggtgatttag ttctgttccg tgccggttca
      661 acgggacgcc atgtcggtat ttatatcggc aacaatcagt ttgtccatgc ttccaccagc
      721 agtggtgtta ttatttccag catgaatgaa ccgtactgga agaagcgtta caacgaagca
      781 cgccgggttc tcagccgcag ctaataaacc gtttggatgc aatcccttgg ctatcctgac
      841 gagttaactg aaagcactgc ttaggcagtg cttttttgtt ttcattcatc agagaaaatg
      901 atgtttccgc gtcttgatcc aggctatagt ccggtcattg ttatctttta aatgttgtcg
      961 taatttcagg aaattaacgg aatcatgttc atacgcgctc ccaattctgg acgtaagctc
     1021 ctgcttacct gcattgttgc aggcgtgatg attgcgatac tggtgagttg ccttcaattt
     1081 ttagtggcct ggcataagca cgaagtcaaa tacgacacac tgattatcga cgtacaaaag
     1141 tatctcgata cctattttgc cgacctgaaa tccactactg accggcttca gccgctgacc
     1201 ttagatacct gccagcaggc taaccccgaa ctgaccgctc gcgcggcgtt tagcatgaat
     1261 gtccgtacgt ttgtgctggt gaaagataaa aaaacattct gttcatctgc gactggtgag
     1321 atggacattc cactcaatga attgattccg gcgctcgaca ttaataaaaa cgtcgatatg
     1381 gcgatcttac ccggcacgcc gatggtgccg aacaaacccg caatcgtcat ctggtatcgc
     1441 aaccctttgc tgaaaaatag cggtgtgttt gccgctctga atctcaacct gacgccttct
     1501 ctcttttata gttcacggca ggaagattac gatggcgtcg ccctcattat tggcaatact
     1561 gcgctatcta ccttttcttc acgtttgatg aacgttaatg aattaaccga catgccggtc
     1621 cgtgaaacta aaattgcggg cattcctctg accgttcggc tttatgcgga tgactggaca
     1681 tggaacgatg tgtggtacgc atttttactg ggcggcatga gtggaactgt cgttggcctg
     1741 ctctgctatt acctgatgag cgtacgtatg cgccccggca gagaaatcat gaccgccatc
     1801 aagcgcgaac aattttacgt ggcgtatcaa ccggtggtgg atacacaagc tttgcgagta
     1861 acgggcctgg aagtactgct acgctggcgg catcctgtcg cgggagaaat tcccccggat
     1921 gccttcatta actttgccga atcgcaaaag atgattgtgc cgctgactca gcacctgttt
     1981 gagttaattg cccgcgatgc cgcagaatta gaaaaagtgc tgccagtagg cgtcaaattt
     2041 ggtattaaca ttgcgccgga ccatctgcac agcgaaagct ttaaagcaga tatccagaaa
     2101 ctgctcactt ccctacccgc acaccatttc cagattgtgc tggaaattac cgagcgcgat
     2161 atgctgaaag agcaagaagc cacacaactc ttcgcctggc tgcactcggt cggcgtagaa
     2221 attgctattg atgacttcgg caccgggcac agcgcgctta tctatcttga gcgttttacg
     2281 ctcgattatc tgaaaattga ccgtggattt atcaacgcca tcggtacgga aacgatcact
     2341 tcacccgtac ttgacgcggt gctgacgctg gcgaaacgtc tcaatatgct gacagttgct
     2401 gaaggggtcg aaacgccgga acaagcgcga tggctaagcg aacgcggcgt taatttcatg
     2461 caaggctact ggattagtcg cccgttaccg ctggacgatt ttgtccgctg gctgaagaaa
     2521 ccgtatacgc cgcagtggta aggtgtgctt acgtccctta ttattcatag tgaaagcatg
     2581 ccggattgcg gctaatgatg agtaaaagga aatccgttgc agatgattgt gcgcatactg
     2641 ctgctgttta tcgctctgtt cacctttggt gtgcaggcgc aggctatcaa ggaaagctat
     2701 gcctttgccg tgctgggcga accccggtac gcgtttaatt tcaaccattt tgattatgtg
     2761 aaccccgccg cgccaaaagg tgggcagata acgttgtcag ccctcggcac cttcgatact
     2821 ttcaaccgct atgcactgcg cggcaacccg ggcgcacgca ccgagcagct gtacgacacg
     2881 ctatttacga cttccgatga cgaaccaggc agttattacc cgctgattgc tgaaagcgca
     2941 cgctatgctg acgattattc ctgggtggag gtcgctatta atccgcgcgc ccgttttcat
     3001 gatggttcgc ccattactgc ccgcgatgta gagtttactt ttcaaaaatt tatgaccgaa
     3061 ggcgtgccgc aatttcgtct ggtctacaaa ggcaccaccg tcaaagccat tgcgccgtta
     3121 accgtgcgca ttgagttagc taaacccggc aaagaagata tgttgagtct gttttcgctg
     3181 ccggtatttc cagaaaagta ctggaaggat cacaaactta gcgacccgct cgccacgcct
     3241 ccgcttgcca gtggtccgta ccgcattacg tcctggaaaa tggggcaaaa tattgtctat
     3301 tcccgcgtga aagattactg ggcagcaaac ttaccggtaa accgcggacg ctggaatttc
     3361 gacaccattc gctacgatta ttacctcgat gataatgtcg cctttgaagc gtttaaagca
     3421 ggtgcctttg atttgcgtat ggaaaacgac gccaaaaact gggccacgcg ttataccggt
     3481 aaaaatttcg ataaaaaata catcatcaaa gatgagcaaa agaacgaatc agcccaggat
     3541 acgcgctggc tggcgtttaa tatccaacgt ccagtattca gcgatcgccg ggtgcgggaa
     3601 gcaatcactc tcgcctttga ctttgaatgg atgaacaagg cgttgtttta caatgcctgg
     3661 agtcgcacga acagttattt tcagaatacc gaatacgcgg ccagaaatta ccccgacgcc
     3721 gcggagctgg tgcttctggc accaatgaaa aaagatctac cgccagaagt cttcacacaa
     3781 atctaccagc cgccggtatc caaaggcgat ggctacgatc gtgacaacct gttaaaagcc
     3841 gacaaacttc tcaacgaagc gggctgggtg ctgaagggtc agcaacgcgt taatgccaca
     3901 acgggtcagc cactcagctt tgaattattg cttcccgcaa gcagcaatag tcagtgggta
     3961 ttgccgttcc agcacagcct gcaacggctg ggtatcaaca tggacattcg caaggtggat
     4021 aactcgcaaa tcactaaccg catgcgcagt cgcgactatg acatgatgcc gcgcgtatgg
     4081 cgggcgatgc cgtggcccag ttccgattta cagatttcct ggtcatcgga atatatcaat
     4141 tccacttata atgcccccgg cgtgcaaagc ccggttatcg actcgctgat caaccaaatt
     4201 attgccgcgc agggaaataa agaaaaatta ctgccgttgg ggcgagcact ggatcgcgta
     4261 ttaacgtgga attattacat gctgccaatg tggtacatgg cggaagaccg tctcgcctgg
     4321 tgggataaat tctcccagcc ggccgtgcgc cccatctata gcctcggtat cgatacctgg
     4381 tggtatgacg tcaataaagc ggccaaactg ccgtccgcca gcaaacaggg agagtagatg
     4441 ggcgcttacc tgattcgccg tctgttgctg gtgatcccaa cattatgggc gattatcacc
     4501 atcaactttt tcatcgtgca aattgcgcct ggcggtccgg tcgaccaggc catcgccgcc
     4561 attgagtttg gtaatgccgg agtattaccc ggcgcaggcg gtgaaggtgt tcgtgccagc
     4621 catgcgcaaa cgggtgtcgg caatatcagc gacagtaagt accgtggcgg acgcggatta
     4681 gatccagaag tgatcgctga gatcactcat cgctacggtt ttgataagcc gatccacgaa
     4741 cgttacttca aaatgctctg ggactacatc cgctttgatt ttggcgatag cctgtttcgc
     4801 agcgcctcgg tgctgacgct gattaaagac agtctgccgg tttccatcac cctcggattg
     4861 tggagcacgc tgattatcta tctggtgtcg attccgttag gcattcgcaa agctgtttat
     4921 aatgggagcc gctttgacgt ctggagtagc gcatttatca tcatcggcta cgccattccg
     4981 gcctttttgt ttgccatcct gctgattgtc ttcttcgcgg gcggcagcta tttcgacctg
     5041 ttccctctac gcggcctggt ttccgctaac tttgattcgc tgccgtggta tcagaaaatc
     5101 accgattatc tgtggcatat cacgctgccg gtgctggcga cagtgattgg tggctttgcg
     5161 gcgctgacca tgctgacaaa aaactcattc cttgatgaag tgcgcaagca atacgtggtg
     5221 accgcgcgtg cgaaaggggt aagtgaaaaa aatattctct ggaaacatgt gttccgcaac
     5281 gccatgctgc tggtgattgc cggttttccg gcgacgttta tcagcatgtt ttttaccggc
     5341 tcgctgctga ttgaggtgat gttttcactc aatggtctgg gcttactggg ctacgaagcg
     5401 accgtctcgc gcgattatcc tgtaatgttt ggtaccttgt atattttcac cctgattggc
     5461 ctgctgctga atattgtcag tgatatcagc tatacgctgg tcgatccgcg tatagatttt
     5521 gagggacgtt aatgtcgcga ctcagccccg tcaatcaggc ccgttgggcg cgttttcgcc
     5581 ataaccgtcg cggctactgg tcgttatgga ttttcctcgt tttgtttggt ttgagtttgt
     5641 gttctgaact tatcgccaac gataaaccgt tgctggtacg ttatgacggc agttggtatt
     5701 tcccgttgtt gaaaaactac agcgaaagcg attttggcgg cccgctggca agtcaggctg
     5761 attatcagga cccgtggctg aaacaacggc tggaaaataa cggctgggta ctgtgggcac
     5821 cgattcgctt tggtgctacc agtatcaact ttgctaccga taagcccttc ccttctccac
     5881 cctcccggca aaactggctg ggaacggatg ccaacggcgg cgatgtgctg gcacgtattc
     5941 tctatggcac gcggatctcg gttctgtttg gcctgatgct gactctctgt tccagcgtga
     6001 tgggcgtgct ggcgggggcg ctacaaggct attacggcgg taaagtcgat ctctgggggc
     6061 aacgctttat tgaagtatgg tcggggatgc cgacgctgtt tttgattatt ttactttcca
     6121 gcgtcgtaca gcctaacttc tggtggctgc tggcaattac tgtcttgttt ggctggatga
     6181 gtctggtcgg cgtggtgcgg gcggagtttt tacgcacccg taatttcgac tacattcgtg
     6241 cggcgcaggc gcttggcgtc agcgatcgca gtatcatcct gcgtcatatg ttgcctaatg
     6301 ccatggtcgc gaccctcacc tttttaccgt ttattttatg tagttcgata accaccctga
     6361 cctcactcga tttcctcggc ttcggtctgc cgctcggttc accgtcactc ggtgaactgc
     6421 tgttacaagg gaaaaataac cttcaggctc cgtggcttgg gatcaccgcc ttcttgtcgg
     6481 tggcgatatt attgtctttg ctgatcttta ttggtgaagc cgtccgcgac gcatttgatc
     6541 ctaataaggc ggtgtagcat gacgcaaact ctgttagcga ttgaagattt gtcggtgggt
     6601 tttcgccatc agcaaaccgt acgtacagta gtcaatgatg tttcactaca gattgaggct
     6661 ggcgaaacgc tggcgctggt gggtgagtca ggttcaggca aaagcgttac cgcgctgtca
     6721 attttacgcc tgctcccttc cccgccggtt gaatatctct ccggcgatat tcgttttcgt
     6781 ggcgaatcgc tgcttcatgc cagcgatcaa acgttgcgcg gtgtacgcgg taataagatc
     6841 gccatgattt ttcaagagcc gatggtgtcg ttaaatccat tgcataccct ggaaaaacag
     6901 ctttatgaag tgctttcact ccaccgcggg atgcgtcggg aagcggctcg aggcgaaatt
     6961 cttaactgcc ttgatcgcgt tggtatccgc caggcggcaa aacggctgac agattatccg
     7021 catcagctct ccggcggcga acgccagcgg gtgatgattg cgatggcgct gttaacgcga
     7081 ccggaattat taattgccga tgaaccgacc accgcactgg acgtctctgt ccaggcgcag
     7141 attttacagc tgttgcgcga actgcaaggc gagctgaata tgggcatgct gtttattact
     7201 cataacctca gcattgtcag aaaactggcc caccgcgtgg cggtaatgca aaacggtcgc
     7261 tgtgtcgagc aaaataacgc cgctacgcta tttgcctcac ccactcatcc ttacacacaa
     7321 aagctactca acagtgaacc gtcaggcgac ccggtgccgt tgccagaacc tgcctcaacg
     7381 ttgctggatg ttgaacagct tcaggttgcc ttccccattc gcaaagggat tttgaagcgc
     7441 attgtggatc ataatgtggt ggtgaaaaac atcagtttta cgctacgagc gggtgaaaca
     7501 ctgggtttag tgggcgagtc cggttccggg aaaagtacga cgggactggc gctgctgcga
     7561 ctgattaatt ctcagggcag catcatcttt gacggtcagc cactgcaaaa tttaaatcgc
     7621 cgccagctgt tacctattcg tcatcgcatt caggtggtat ttcaggatcc aaactcctcg
     7681 ctcaacccac gactcaacgt tttgcagatt attgaggaag gcttacgggt tcaccagccg
     7741 acgctttctg ccgcacaacg cgaacaacaa gtgatagccg tgatgcatga agtgggatta
     7801 gatcctgaaa cacgccaccg ttatccggcg gagttctctg gtggtcagcg acaacgtatt
     7861 gcgattgcca gggcattaat tcttaagccc tcgctgatca tacttgatga accgacatca
     7921 tcactcgaca aaacggtaca ggcgcaaata ttgacgctat tgaaatcatt gcaacaaaag
     7981 catcaactgg cctatttgtt tatcagccac gatttgcacg ttgtccgcgc gttatgtcat
     8041 caggttatcg tactgcgaca aggggaagta gtggaacaag gaccgtgcgc gcgcgtgttt
     8101 gccacaccgc agcaggagta tacgcgtcag ctactggcgt tgagctgacg cttaaaaagg
     8161 attgtagttt gaaaagggtt cggcaattgc cacaccaaaa tttttcaatc ggcatgttgc
     8221 agcaaactcg tcctggcgat ttacaaacag gcacggctca ccttcacact ccagcaccga
     8281 acatggaact tcgatgtcgc ttaacgcctg gcttagttta tacatcaccg accatgcatt
     8341 atcaccatcc gggctaagca atttaagagc caccaggctg ttgtcaccgc acggtccatt
     8401 ttgcggaatc ggttcaacct tcgaacctgc gaaaccagca gaccagcgat agttctgcgg
     8461 cagtcgatgg acgattgaga gttgtaatga agtcacttat tttccccgga agcacattca
     8521 cttcacaatt tgtttacatc aattttaaca catcatcaac aatccgtctt tatacagatc
     8581 gtaaagattg atgcctcgcc tgcgtggcct ctatggctct gatttaagta atatctgcgg
     8641 gcgtggtcac gtttgcgggg ctatatgtac ccgtttctgc gatctaactc aaccttttta
     8701 actacaatga tgtgactttt tacataaatt gattttacat aaaataaaca tatatcgggg
     8761 gaatgataga tttgtggttg acatgcatca acaaaggaag ccttttagct tcctcgttgt
     8821 gcaatagatc accgtttttt cggtcgactg gcgtacagac agaagagaat ggagctgctt
     8881 gcgcagaatg caattgacca aatcatcggc catgcagagt taaaggtcgc aagagaaagc
     8941 aatgcgccaa caattgcccc tatgccaaaa cggaaggttc ctgccagcga agatgccgtt
     9001 cccgccatat ggggaaactc atcaagaatg accgccatcg cattggatga caccatcgac
     9061 acgcagccca caaacgccgc aacgccaacc accagcgacc agaatcccag ccccagcagc
     9121 gcactgatga ccatccacgc tgccataata aattgtatcc acaaccccga gcggaacata
     9181 tttaacgcgc caatgcggcg aacgaagcgg ctgttaaaaa tggtcatcac gaacaggaaa
     9241 acaatgttta gtgcaaagta ataaccaaag ttttccggcg cgacgtggtt aatttcaatg
     9301 tagacaaacg gtccggcgct taagaacgag aacatcccgg caaagctgaa accactggca
     9361 agcatataac tcaggacacg cttatgacgg aacagcgccg caaagttacc aatagtggta
     9421 cgaatgtgaa aaggctggcg acgctccggt ggtaaggttt ctttaatcag gaagaaaatc
     9481 attgccgaag ccagaatcgc cgctaatgcc aggatccaga agatgtaatg ccagctcagc
     9541 cacaccagca cccaaccgcc gacaatcggt gccatcagcg gtgcaatagt ggtcaccagc
     9601 atgacaaacg acatcatccg cgagaactct tctttcgggt aaatatcgcg catcagggcg
     9661 ttaatgacca cgctggccgc agccgccgcc agcccgtgga agaaacgcat cacaatcagc
     9721 tgatcgatgg tgtttgccaa cgcacacgcc accgcggcgg cggcaaacac cagcgtaccg
     9781 ccgagcacca ccggcttacg cccgaagctg tctgccatcg gcccgtagat taactgcccc
     9841 aacgcaaagc ccagaatata agtactgagg gtcatctgcg tactgcccgc aggtacgcca
     9901 aactgcgctg aaattaccgg tagcgcgggc agatacatat caatcgacag cggcatcaac
     9961 atggccagca ggccaaggat aaaaacaata gcaaacgacg aatgctgtcg ggtggtcaca
    10021 acgggctcct gaaagtcatg gaaaagttag acgacgctgg caatttcttc ttcagttaac
    10081 ggacgatatt caccgggggc taaatcagca tccagcgtaa taccgccaat acgttcacga
    10141 tgcagctcaa ccacgtggtt acccacggcg gcgaacatgc gtttcacctg atgataacgc
    10201 ccttcgctga tggtcagacg aacctgcgtt ggggtaatca cttccagcac cgcaggctta
    10261 gtgagatctt tttcgttatg cagctgcacg cctttagcaa attgctctgc cgtatcgtca
    10321 gctacaggtg attccagtgt caccagatag gtcttctcgc aatgatggcg cggagaagta
    10381 atgcggtgcg accactgacc atcatcagtc atcagcacca gaccggtggt atcgatatcc
    10441 agacgtcctg cggcgtgcag tttccaggct accggttcat ccagaaaata gagcaccgtt
    10501 gggtgatcag ggtcgtccgt ggagcaaaca tagccctgag gcttattgag catgaagtaa
    10561 cgtggaccat gttgctgcgc cagcgggttg ccatcgtaag cgacatcatg ttcaggaagc
    10621 agtttgaacg ctgcattacg aacaatttcg ccatcgacgg tgacacgatt gccgcggatt
    10681 tcacgcccgg caatagcacg gctaacgccg agttgctgtg cgataaattt atcaagtcgc
    10741 atgtgtgtga ttttgcctgt aaaaacggag gtcgggcatt gacccgaaaa tctgaactgt
    10801 tgtctgccca gtatagcggt ctaataacat ccctcaaggg aaaaagatcc atggcatact
    10861 attagcagaa taatctacct acgcgagacc atgattttta cactccgccc atatcagcaa
    10921 gaagccgtgg atgccacgct caaccatttt cgtcgtcata aaacccctgc cgttatcgtg
    10981 ctgcccaccg gcgcaggtaa aagcctggtg attgccgaac tggcacggct ggcgcgtggt
    11041 cgtgttctgg tgctggcaca cgtcaaagaa ctggtggcgc aaaaccatgc aaagtatcag
    11101 gcgctggggc tggaagccga tatttttgcc gctgggttaa agcgcaaaga gagccacggt
    11161 aaagtggtat ttggcagcgt gcagtcggtc gctcgtaatc ttgatgcctt tcagggtgaa
    11221 ttttcgctgt tgattgtcga tgaatgccac cgtattggtg acgatgaaga gagccagtat
    11281 cagcaaatcc tcactcacct gactaaagtg aatccccact tacgcctgct ggggctgact
    11341 gccacgcctt ttcgattggg caaaggctgg atctaccagt tccattatca cggcatggta
    11401 cgcggcaatg agaaagccct tttccgtgac tgcatttatg agctgccgct gcgttatatg
    11461 attaaacacg gctatctgac gccgccagaa cgactggata tgccagtagt gcaatacgat
    11521 ttcagccgct tgcaggcaca gagtaacggg ctgttcagcg aagctgatct caaccgtgag
    11581 ctgaaaaaac agcaacgcat tacgccacat atcatcagcc agatcatgga gttcgccgaa
    11641 aagcgcaaag gggtaatgat tttcgccgcc accgttgaac acgcaaaaga gattgtggga
    11701 ttactgcctg ccgaagatgc tgcactgatt actggcgaca cccccggcgc tgagcgcgat
    11761 gtgttaattg aagattttaa agcccagcgt tttcgctatc tggtcaacgt cgcagtactg
    11821 accaccggat ttgacgcccc gcacgtcgat cttatcgcca ttctgcgccc taccgaatca
    11881 gtgagtcttt accaacaaat tgtcgggcgc ggtctgcgtc tcgctccggg caagactgat
    11941 tgtttaattc ttgattatgc gggtaatcct cacgatctct acgcgccgga agttggtaca
    12001 ccgaaaggca aaagtgacaa cgttccggta caggttttct gccctgcctg cggttttgcc
    12061 aacacctttt gggggaaaac gaccgccgac gggacattga ttgaacactt tggtcgccgc
    12121 tgtcagggat ggtttgaaga tgacgacggt catcgcgaac agtgtgactt ccgtttccgt
    12181 tttaaaaatt gcccgcaatg taacgcagaa aatgatattg ccgctcgccg ctgccgcgaa
    12241 tgtgacacgg tactggttga tccggatgat atgttaaaag cggcgctacg actgaaagat
    12301 gcgctggtat tacgctgtag cggcatgtct ttgcagcatg ggcacgacga gaaaggcgaa
    12361 tggttgaaaa tcacctatta cgatgaagac ggcgcggatg tgagtgagcg tttccgtctg
    12421 caaacgcccg cccagcgaac tgccttcgag cagcttttta tccgcccgca tacgcgcaca
    12481 ccgggcatcc ctctgcgctg gatcaccgcc gccgatatcc tcgcccagca agccttactg
    12541 cgacatccgg attttgtcgt cgcccgcatg aaaggtcagt actggcaggt gcgtgaaaaa
    12601 gtgttcgatt acgaaggtcg ttttcgtcgg gcgcacgaat tacgcggtta atggtagttt
    12661 tgattgatgt ataaggcgat tgagtataga atctcgcccg cttttgcata cgcaaagcag
    12721 atcacttacc tgttgctggg tcgcctgtag caggattaat ttaagagaga aagaaatgtt
    12781 tactatcaac gcagaagtac gtaaagagca gggtaagggt gcgagccgcc gcctgcgtgc
    12841 cgctaacaag ttcccggcaa tcatctacgg tggcaaagaa gcaccgctgg ctatcgagct
    12901 ggatcacgac aaagtcatga acatgcaagc taaagctgaa ttctacagcg aagttctgac
    12961 catcgttgtt gacggtaaag aaatcaaagt taaagctcag gacgtacagc gtcacccgta
    13021 caaaccgaag ctgcagcaca tcgacttcgt tcgcgcttaa ttgctgaata agttgtaaaa
    13081 aaccccgctc cggcggggtt ttttgtatct gcagattatg cctgattacg gtattgctat
    13141 tttttgcagg ccagataagg cgttcacgcc gcatctggca ttcagaacac aaaaccgatt
    13201 aattgccgcc agacgtccgg cgctgcaatt ggtcgcgcaa attcggcggc gtgcctttaa
    13261 tggtcaaggt atcggttgct ggatcccaga agatgcgttc acctaacagc atcgcatcaa
    13321 agttaatagt caagccaccg ccgctaccgg caaacttcgt caactgacgc agtgtgctac
    13381 ggtctgccgg gaagctctct tccagttcgt agcctttttc agccgcaaac tccgtgaagc
    13441 tcacttcgct tacgcctgcc agctctttcg acagcgattc cagttcgatc tcttcgcctg
    13501 cctgcagctg ctcattacag tagctataaa cctgctggcg cacgttctgt cgctctgctt
    13561 tatccagttg tgcttcggca gtgaaatcat caaccgcttg caacagccca cggttttgcg
    13621 ctttcgcgtt caatccttcg ctggccccga ggaaatccat aaagaaatcc gccactttgc
    13681 gccctactcg ccctttcagg aaagtgagat agcgggtgga ctctggattg gtttcccatt
    13741 cggttaaatc gatacgcgca acaatatccg catgattgat atcaagataa tgggttgggt
    13801 tgatgtcgag attttcgtta acgcgcatac tgctcaggtt gctcagaact gccaccagca
    13861 gatactccac cgccagataa cggtagtggc aaaacagcac gaatccaccg tcagcaaaag
    13921 gatatttcgc taattcgtca cgcaggcgcc cggttgccgc gcggctaaat gccagaaagt
    13981 cctcttcgcc ctgacgctgt aagcgcagcg tctgcgccag ttcactctct tcgctaaaca
    14041 agccgtaagc tttatttttg gcgctataga cccgatgcag ttctgccacc atctcaacga
    14101 cggtttctgt cggttccagc aatgaatcgc gcaacaccag ctcaaggttc tgctcatcac
    14161 gcttgataag ctggtgcagg gcaatctggt tgatatccag actcatgata aactctcctt
    14221 taagaccggg cggtattcaa ccaccgccgg ttaagcgacg caataaaaga tggcggtcat
    14281 tttacagaag gcagcgtaac gagaatgatt taacgcataa aaaagcagaa aaaaaaccgt
    14341 tgctacggta atatgttgcc ctttcatcaa ctaactgatt tcgatttatg ccacaaattt
    14401 cccgctacag tgatgaacag gttgaacaat tgctcgcaga gctgctcaac gtactggaaa
    14461 aacataaggc tccgaccgac ctttccctga tggtgttagg caacatggtc actaacctta
    14521 tcaacaccag cattgccccg gcccaacgcc aggcaattgc caactctttt gcccgcgcct
    14581 tacagtcctc tatcaacgaa gacaaagcgc actaagggaa acagataact ggttatggta
    14641 actcatcgtc agcgctaccg tgaaaaagtc tcccagatgg tcagttgggg gcactggttt
    14701 gcactgttca atattctgct ttcgctcgtc attggcagcc gttacctgtt tatcgccgac
    14761 tggccgacaa cgcttgctgg tcgcatttat tcctacgtaa gcattatcgg ccatttcagc
    14821 ttcctggtgt tcgccaccta cttgctgatc ctcttcccgc tgacctttat cgtcggctcc
    14881 cagaggctga tgaggttttt gtccgtcatt ctggcaacgg cgggaatgac gctattactg
    14941 atcgatagcg aagtctttac tcgtttccat ctccatctta atcccatcgt ctggcaactg
    15001 gttatcaacc cagacgaaaa tgagatggcg cgcgactggc agctgatgtt catcagcgtg
    15061 ccggttattt tattgcttga actggtgttt gcgacgtgga gctggcaaaa gctgcgcagc
    15121 ctgacgcgtc gtcgacgctt cgcgcgcccg ctggccgcat tcttatttat cgcctttatc
    15181 gcctcgcatg tggtgtatat ctgggccgat gccaacttct atcgcccgat caccatgcag
    15241 cgcgctaacc tgccgctttc gtacccgatg acggcgcgac gttttcttga gaagcatggt
    15301 ctgcttgatg cgcaggagta tcaacgccgt cttattgagc aaggtaatcc agacgccgtt
    15361 tccgtacagt atccgttaag cgaactgcgc tatcgcgata tgggcaccgg gcagaatgtg
    15421 ctgttgatta ctgtcgatgg cctgaactac tcacgcttcg agaagcagat gcctgcgctg
    15481 gcaggttttg ctgagcaaaa tatttcgttc acgcgccata tgagctccgg caacactaca
    15541 gacaacggca tctttggcct gttctatggc atctcgccga gctatatgga cggcattctg
    15601 tcgacccgta cgcctgcggc attaattact gcgcttaatc agcaaggcta tcagctgggg
    15661 ttattctcgt cagatggctt taccagcccg ctgtatcgcc aggcattgtt gtcagatttc
    15721 tcgatgccga gcgtacgcac ccaatccgac gagcagaccg ccacgcagtg gatcaactgg
    15781 ctgggccgct acgcacaaga agataaccgc tggttctcgt gggtctcttt caatggcact
    15841 aacattgacg acagcaatca gcaggcattt gcacggaaat atagccgggc ggcaggcaat
    15901 gtcgatgacc agatcaaccg cgtgctcaat gcactgcgtg attctggcaa actggacaat
    15961 acggttgtga ttatcactgc cggtcggggt attccgctga gcgaagacga agaaaccttt
    16021 gactggtccc acggtcatct gcaggtgcca ttagtgattc actggccagg cacgccggcg
    16081 cagcgtatta atgcgctgac tgatcatacc gatctgatga cgacgctgat gcaacgcctg
    16141 ctacatgtca gcacacctgc cagcgaatat tcgcaaggtc aggatttgtt caaccctcaa
    16201 cgccgtcatt actgggttac tgcagcggat aacgatacgc tggcaattac caccccgaaa
    16261 aagacgctgg tgctgaacaa taacggtaaa taccgcactt acaacttacg tggtgaaaga
    16321 gtgaaagatg aaaaaccaca gttaagtttg ttattgcaag tgctgacaga cgagaagcgt
    16381 tttatcgcta actgattaat tataaatcag ttagcgaaat atcttacttg caatcggtgt
    16441 ggaaaacggt agtattagca gccacgagtc ggcacgtagc gcagcctggt agcgcaccgt
    16501 catggggtgt cgggggtcgg aggttcaaat cctctcgtgc cgaccaaatc gcccaccgga
    16561 tatcaccgga aagcaccgga taagacaggg ataacccgct aatcacttga ttggcgggtt
    16621 tttttatgtt ttttagtcac cgaacatcac cggatttata agctatcagc cggatttttt
    16681 agttatacgt ttagttatac gggctggcgt ataactaaag ccgtataact aaaaccctgt
    16741 tttttcgtgt ggaatcatag acatatggcg agaaaaacac acccattaac cacagtgcag
    16801 atcaaagcag ccagaccagc gcaaaaggag tacaccctgc aggacggcgg agggcttttt
    16861 ctcctggtca aaccgtctgg atcaaaactc tggagatttt cctactaccg accatcggac
    16921 aaaaaaagaa tattgctgag ttttggatcg cttgaagatg tttccctggc tgatgccaga
    16981 aaacgccgta gcgagtacag gacgttaatc agtgccggaa ctgacccgca ggaccacgag
    17041 aggcaaaaaa gagagacaga ggcccgaaga caagggaaca cgttcgaaaa tgtggcggcg
    17101 gcatggtacc aggtgaaaat cagccagaat ctggccccca acacgattaa agacatctgg
    17161 cgttcgctgg ataaatatgt attcccgttc atcggcaaca cgccaataga taccctcacc
    17221 gcccgaaggt tcgttgaagt gcttaccccc atcaaggagc gcggcaacct ggaaacactc
    17281 aagcgggttt tacagcgcgt taatgaggta atggattacg ccgccaacag tgggctgatt
    17341 gatgccaatc cggctatgaa tgtgcgtaag gcgtttccct cacctgtaaa aaaacatatg
    17401 ccaacaatcc gccccgaaca gctgcccgag cttatgcagg ctttatcagt atcggcaaca
    17461 gaacggcaga ccagattact gattgaatgg cagttactga ccgtaacccg tcccgccgaa
    17521 gcgtcatcaa cgcggtggga tgaaatcaac ctggacgcga agcaatggac gatacctgcc
    17581 ggacgcatga agatgcgcag ggatcacgtt atcccgcttt ccggtcaggc tatggcggtg
    17641 ctggaggcca tgaaaccaat cagccaccac cgcaattacg ttttcccaag tctgaaagac
    17701 ccacagcagc cgatgaacag ccagacagct aacgcagcat tgcggcgtat gggattcgct
    17761 ggcgtgctgg tgtctcatgg attacgcgcc atattcagca cagcagcgaa cgaggaagga
    17821 ttcgagccgg acgtaataga ggcggcactt gcccacgtcg acaccaacga agttagacgg
    17881 gcatacaacc ggagcaacta catagaaaaa cgcatcgtgc tgatgcgctg gtggggcgaa
    17941 tttgtcgagg ctgcggcgac gggcgtaacc ctcgccagtg gtaaaagggg tatccgagcc
    18001 gtgtagctgt acagaaaacc agtaaaaact acgaaaacca tgtaaaaccg tcgtataatt
    18061 gcatcaaatt taacgacaag gccgtgaaac atgaaaccgt taagatgcaa aaaaatatca
    18121 gatgcaattg cgacgggctg caactggccc tgatggttca gcatgaattt tggtcaacct
    18181 acgatccgga ggacagaacg acggccccat caaaaaaaca ggtagtagat tttctggtat
    18241 cccgtggcgc ttccagaaat ctggcggtaa gtattgataa ggtcgtacgt ccggcat
//
LOCUS       DAZGLJ010000083        18210 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000083 DAZGLJ010000000
VERSION     DAZGLJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.083"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(185..991)
                     /gene="trpA"
                     /locus_tag="ACHK49_002474"
     CDS             complement(185..991)
                     /gene="trpA"
                     /locus_tag="ACHK49_002474"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO9263108.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(991..2184)
                     /gene="trpB"
                     /locus_tag="ACHK49_002475"
     CDS             complement(991..2184)
                     /gene="trpB"
                     /locus_tag="ACHK49_002475"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO9263109.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2196..3557)
                     /gene="trpCF"
                     /locus_tag="ACHK49_002476"
     CDS             complement(2196..3557)
                     /gene="trpCF"
                     /locus_tag="ACHK49_002476"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO9263110.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKSSPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3558..5153)
                     /gene="trpD"
                     /locus_tag="ACHK49_002477"
     CDS             complement(3558..5153)
                     /gene="trpD"
                     /locus_tag="ACHK49_002477"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO9263111.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5153..6715)
                     /gene="trpE"
                     /locus_tag="ACHK49_002478"
     CDS             complement(5153..6715)
                     /gene="trpE"
                     /locus_tag="ACHK49_002478"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO9263112.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGMENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNMLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6737..6830)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6807..6851)
                     /gene="trpL"
                     /locus_tag="ACHK49_002479"
     CDS             complement(6807..6851)
                     /gene="trpL"
                     /locus_tag="ACHK49_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO9263113.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6989..7870
                     /gene="rnm"
                     /locus_tag="ACHK49_002480"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6989..7870
                     /gene="rnm"
                     /locus_tag="ACHK49_002480"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO9263114.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7867..8487
                     /gene="yciO"
                     /locus_tag="ACHK49_002481"
     CDS             7867..8487
                     /gene="yciO"
                     /locus_tag="ACHK49_002481"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO9263115.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDKPVVVREGVGDVKPFL"
     gene            8588..9460
                     /gene="rluB"
                     /locus_tag="ACHK49_002482"
     CDS             8588..9460
                     /gene="rluB"
                     /locus_tag="ACHK49_002482"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO9263116.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(9500..10090)
                     /gene="cobO"
                     /locus_tag="ACHK49_002483"
     CDS             complement(9500..10090)
                     /gene="cobO"
                     /locus_tag="ACHK49_002483"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO9263117.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(10087..10845)
                     /gene="yciK"
                     /locus_tag="ACHK49_002484"
     CDS             complement(10087..10845)
                     /gene="yciK"
                     /locus_tag="ACHK49_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO9263118.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFLLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            11065..12114
                     /gene="sohB"
                     /locus_tag="ACHK49_002485"
     CDS             11065..12114
                     /gene="sohB"
                     /locus_tag="ACHK49_002485"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO9263119.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(12150..12401)
                     /gene="yciN"
                     /locus_tag="ACHK49_002486"
     CDS             complement(12150..12401)
                     /gene="yciN"
                     /locus_tag="ACHK49_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO9263120.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            12781..15378
                     /gene="topA"
                     /locus_tag="ACHK49_002487"
     CDS             12781..15378
                     /gene="topA"
                     /locus_tag="ACHK49_002487"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO9263121.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            15588..16562
                     /gene="cysB"
                     /locus_tag="ACHK49_002488"
     CDS             15588..16562
                     /gene="cysB"
                     /locus_tag="ACHK49_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO9263122.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            16881..17021
                     /gene="ymiA"
                     /locus_tag="ACHK49_002489"
     CDS             16881..17021
                     /gene="ymiA"
                     /locus_tag="ACHK49_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO9263123.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            17024..17191
                     /gene="yciX"
                     /locus_tag="ACHK49_002490"
     CDS             17024..17191
                     /gene="yciX"
                     /locus_tag="ACHK49_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO9263124.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            17305..17400
                     /gene="ymiC"
                     /locus_tag="ACHK49_002491"
     CDS             17305..17400
                     /gene="ymiC"
                     /locus_tag="ACHK49_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO9263125.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            17564..>18210
                     /locus_tag="ACHK49_002492"
     CDS             17564..>18210
                     /locus_tag="ACHK49_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFO9263126.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTT"
BASE COUNT         4472 a         4563 c         4787 g         4388 t
ORIGIN      
        1 actggtgtta tgttgcggga ttaatttgtt tgattatatt actgttgggc ggaaaaatga
       61 cgtaagttga cgctcgaccg gggtaagcga aacggtaaaa agataaatat taaatgaatt
      121 taggattttt ccggcttcat taaagaaagt taaaatgccg ccagcggaac tggcggctgt
      181 gggattaact gcgcgtcgcc gctttcatcg gttgtacaaa aactttcagt gccgccagca
      241 ttttctctgg ctcattaata tgttgctcga tgattttaac aatggccgaa ccagaaatcg
      301 cgcccgcagc tcctgcatca atcgctgctt ttacctgatc cggggcggaa ataccaaatc
      361 cctgcaatgg aggtgcagcg ttgtactctt tcagcttcgc aaccagatga ttgaggggta
      421 acgcggcgcg gttttctgcg ccggtcacgc ctgctcgtga cagcaaatag gtgtaaccac
      481 gaccgtaaga ggctatctgg cgcagcaggt cgtcatcggc atttggcggg cagatgaaga
      541 taggtgcgac attatgacgc aacgcggcct ggcggaaggg cgcggactct tcaactggca
      601 catcggcaac cagcaccgaa tcgacgccga ctttttcgca ctgggcataa aactcatcaa
      661 tgcctttgtt aaacaccaga ttggcataca tcaacaggcc aatgggaatg gtcgggtgtt
      721 tctggcgaat cagtgccagc atttcaaaac attgtgccgg agtcacacct gccgcaaagg
      781 cgcgcagagt ggcgttttga atcgtcgggc catccgccag tgggtcggag aaggggatac
      841 ctaactccag cgcgtcagca ccggcttcaa ttagcgtatc gataattttc aatgactgct
      901 caatgcccgg atcaccgagc gtgacgaaag gaacgaatgc gccttctttg cgctccttca
      961 actgggcaaa cagagattcg tagcgttcca tcagatttcc cctcgtgctt tcaaaatatc
     1021 gtgaacggtg aagatgtctt tatcgccgcg accggaaagg ttaaccacca gtagctgctc
     1081 tttatccggg ttttcgcgca tcattttcaa cgcatgggcc agggcgtggg aggattccag
     1141 cgccgggatg atcccttcgt gcaggcacag cgttttgaag gcttcaaggg cttcatcatc
     1201 ggtaatagac acgtaatcag cgcgtccagt gctgttaaga tacgcgtgtt gtgggccgac
     1261 agacgggaaa tccagtccgg cggagatgga gtaagattct tcaatctgcc cgtcttcggt
     1321 ttgcatcatc ggcgctttca taccgaaata gatacccacg cgaccatgtt ttagcggtgc
     1381 gccgtgctcg ccagtttcga taccgtgacc acctggctcc acaccaatca ggccgacgtt
     1441 ggtttcattg atgaaatcag caaacatgcc gatggcattc gaaccgccgc caacacaggc
     1501 gataacggca tccggcaggc gaccttctct ttccagaatc tgcgctttgg tttcttcgcc
     1561 aatcatccgc tgaaactcac gcacaatggt cggataagga tgcgggccag ctgcggtgcc
     1621 cagcatatag tgcgcggttt cgtaactacc ggaccagtcg cgcagcgcct cgttacaggc
     1681 atctttcagc gtcgcggaac cgctatgcac cgggatcact tccgcaccca ttaagcgcat
     1741 acgaaaaacg ttaggcgact ggcgttcaac gtctttggca cccatataaa tacggcattt
     1801 caggccgagc agggcgctgg caagggccga cgccacgcca tgctgaccgg caccggtttc
     1861 ggcgatgatt tcggttttac ccatccgctt cgccagcaac gcctgcccca gcacctggtt
     1921 agttttatgc gcgccgccgt gcagcaaatc ttcacgcttg agatacagcg tagtgttcgt
     1981 cccggctgta atgttctggc atttggtcag cgcggttgga cgcccggcat agtttttcag
     2041 caggtcgttg aactgagcct gaaattcagg atctttttgc gcactgacaa aagcttcttc
     2101 cagctggcgc agagcaggca tcaggatttg tggcacgtac atgccgccaa actcaccaaa
     2161 ataggggtta agtaatgttg tcattgttcc tttccttaat atgcgcgcag cgtctggaaa
     2221 accgaggcca aaagacgtgc gtctttgatg cccggttgcg actctacagc agaattaaaa
     2281 tcaagtccgg cgcagccggt ttgtgccgct tccacgcagt tatctgcgcc taagcccccc
     2341 gccagcagaa cgttgccaag cgattgacca tttaatagtg accagtcaaa acgttgcccg
     2401 cttccaccct ggccgttgtc taaaacatat ttatcaacgt gctgaaactc gcgggcgggc
     2461 agggtttcac cgacgcttaa tgctttccag atggcaacat gtgctggcag agcttcacgc
     2521 agcgtatcga tatacagctg ttcttcatta ccatgcagtt gcactgccgc cagcgataac
     2581 accttagctt tgtccaccac atcggcaata tcgtgattgc ggaacacgcc aacatactgc
     2641 aacggtgccg cagccatcac ttcctgcgcc tgttcaacgt tgacgcaacg cggtgatgtc
     2701 gcaacaaaaa tcaacccacc gtaaatcgcg cccgcgtcat aagctgcttt agcatcttgc
     2761 ccacgcgtca ggccacatac tttattctca cccagcaaca cccggcgcac ggcggcgtgc
     2821 aaatcgtcat gggccatcaa cgccgaacca atcagaaaac cgttagcgaa gtggcttaac
     2881 tcgcgcacct gagcgtaagt attgatgccg gattcgctga ttaccgtcac gttgtgcccc
     2941 agtttcggcg caagctcgcg ggtacggttg agatcaatcg acaaatcacg cagatcgcgg
     3001 ttgttgatgc caacgacctt tgcccccaat gcaatggcac gctccagttc ctcttcatta
     3061 ctgacttcgg tcagcacacc catctccaga ctgtgggcga cggcggcaag ctggcgatat
     3121 tgttcgtcat ccagtactga aagcattaat aagcaggcat cagcctggta atagcgcgcc
     3181 agatagatct ggtaaggatc gataatgaag tctttacata aaatcggctg cggggcgatt
     3241 tggctgacga tggggaggaa atcaaagctc ccctgaaaat atttctcatc agtcagcacc
     3301 gaaattgccg aagcgtaatg tttataaatg gcggcaatgc gtgctggatc gaaatcatca
     3361 cggatcacgc cttttgacgg cgacgatttc ttgcactcca gaataaacgc cgtgcgcgca
     3421 ccctgtagcg catcataaaa atgtcgcgtg ctcggctgaa cctcattctg aaaactggcc
     3481 agcggttgct gctgtttgcg ggtttctacc caaatcgcct tgtctgcgac gattttcgct
     3541 aaaacggttt gcatcattta ccctcgtgcc gccagtgcgg taactctgtc gtaagcggaa
     3601 ccactgcgca gtacctcaag aacggtttgc gcattggctt gcagatcttc atggccatgc
     3661 aggcgcatta acatggcgac gttcgcagcg acggctgctt catgggcggc gtcgccttta
     3721 ccttgtaaca agcgtgttaa aatgtcacgg ttttcttccg gtgttccgcc tgccagttgc
     3781 tcctggtggt agggagtcag gccaaaatct tcagcggtca attgatagct cttaatttcg
     3841 ccgtcatgca gctcggcaac gattgtcggc gcgtgtaatg aaacttcatc catcccgccg
     3901 ctgtgcacca ccgccgcgcg ttgatacccc agcacgcgca aggtttcggc aatcggcagc
     3961 accagttccg gactataaac accaattaac gccagcggcg gatgcgccgg gttaatcaat
     4021 ggccccagca cattgaacag ggtgcgggtt ttcagttgct ggcgaaccgg catcgcgtgg
     4081 cggaatccgg tgtgatactt cggcgcaaag aggaaacata cacctaactc atccagcgcc
     4141 tggcgcgatt tatcggcgtt catatcaaga ttaataccga acgccgccag cagatcggac
     4201 gaaccagatt tactggagac gctacggttg ccgtgtttcg ccactttcag cccacaggcc
     4261 gcggcgacaa acgcactggc ggtagaaata ttgatactgt tgctgccgtc accgccagta
     4321 ccgacgatat cagcaaacag ataatccggg cgcgggaacg gcgcggcgtt ttccagtagc
     4381 gcggttgctg ctccggcgat ctcgttcggg tgctcaccgc gaattttcat gctcaccagc
     4441 gccgccgcca gttgttccgg cttcagctcg ccacgcacca ccgctgaaaa cagctggtgg
     4501 ctttcttgtt ggctaagcgt ctgcgcctga tacagttttt ccagaatcgg ttgcagcgtg
     4561 ttggctggct ctagtttctg ctgcgcccag gccagcgttt gttccagcag gcgagcgccc
     4621 tgggtggtga gaatggattc cggatggaac tggaatccac aaacgcgatc cgcatcgtga
     4681 cgtactgcca tcaccatgcc attaaaatgg gcgttgatgg ttaaaccggc cggaatgtta
     4741 ctgccaacca gcgagtgata acgcgccacc ggcagcgggt ttgttaatcc ggcaaacatc
     4801 gcctgaccgt catgttcaat gctcgacgct ttaccgtgaa gaatttcgcc cgcctgaccg
     4861 acatagcccc cgtaagcttc gacaattgcc tgatgtccga ggcaaatgcc aataatgggc
     4921 agcttgccac gcaagcgggt gaggagttcc ggcatacaac cggcttcgct cggcacaccg
     4981 gggccaggag aaagcatcag taccggattg ctcatcgtcg ccaggcgttc aattaaggtc
     5041 tgcgccggaa tatggttgcg gtaaatcacc acattatgcc cattgctgcg caactgatct
     5101 gccaggttgt acgtaaaaga gtcgatatta tcgagcagca gaatgtcagc catcagaaag
     5161 tctcctgtgc atgatgcgcg gtggcaatag cgcgcagtac agcgcgggct ttattacggg
     5221 tttcgtcggc ttccgactgc ggaacagaat caaggactac gccagcaccg gcttgcacgg
     5281 tggcgatacc gttttccacc agcgccgagc ggatcacaat gcaggtgtcg agatcgccat
     5341 gcgcggtgaa ataacctacc gcgccgccgt agctgccgcg gcgacgacct tccgcctcgg
     5401 caattaactg catagcgcgt actttcggcg caccacttaa cgttcccata ttcatacagg
     5461 cgcggtaagc gtgcagggcg tcgagatcgt ggcgcagctc accgaccacg cgggagacca
     5521 agtgcatcac gtaagagtaa cggtcaactt tggtgaggtc ggcgacgtag cggctgccgg
     5581 gggtgcaaat gcgtgccaga tcattacggg cgagatccac cagcatcaga tgttcagaaa
     5641 gctctttatg atcggtacgc atttccagtt cgatgcggct gtcgaggtct ctgtccagtg
     5701 aaccatcggc acgacgaccg cgcggtcgtg ttccggcaat cgggtagatc tcaatctggc
     5761 ggctggtggc gtcatacttg agcgaacttt ccggcgacgc gccaaacagg gtgaaatcat
     5821 tatcctgcat aaaaaacatg tacgggctgg gattactctt tttcagcacg taataggccg
     5881 ccagcggtga cgggcagggc agagagaaac ggcgagatgg caccacctgg aaaatttctc
     5941 cggcgcgaat cgctttttgc aacaaacgca ctacgccacc gaactcttca tcgctctggt
     6001 tacattcaca acgcatatgc ggcacggaaa ccaccggcag cggtggcgcg gtttcggtca
     6061 gttgctggcg aagatcgttc aggcgagcag tgagacgttg tttttcttct tcattcggag
     6121 caaacaggct ggcctgaatg cgggtgcttt ttttctgatg gtcaatcacc atcagcgttt
     6181 cagcgagata aaaacagaaa tcaggacagt tattttccgc tgacaattgc ggtaaatctt
     6241 caaaccccgc cacaaggtca taagagaaca ggccgccgaa gaacattgct tctcgttctt
     6301 ccttcggtac attcagcata ttctgcaata agcggaaagc gtcaaaaacc gaaagggagc
     6361 ataagcgagc gtcttcatcc agcagtggac tgacaggggg gaagcgcagc acacggcagt
     6421 ttggtaattg ttcattttcc atacccgcag gcagggcgtt atccagtagt gtcagcaggg
     6481 cttcgccgtt gccggaaagt gcctggattg tgacagtgtc acctaaagct gtaatgcgca
     6541 gcgcactgtc taccagcagc aggcttttta aatcatcttt gctgtcgata tctgcggatt
     6601 ccagcagcag cgttgccgga cgatccccac acaactggtg aaaaagcgcg gtgggattgt
     6661 cgcgataagc gccttcgcag gttagcagtt cgagagtcgg tttttgtgtt tgcattgtta
     6721 ttctctaatt ttgttcaaaa aaaagcccgc tcattaggcg ggctgggtat ctgattgctt
     6781 tacgcatggt gaatacactg cccgtttcag gaagtgcgcc accaaccttt cagtacgaaa
     6841 attgctttca ttgtcgatac cctttttacg tgaacttgcg tactagttaa ctagttcgat
     6901 gattaattgt caatagctca tttcagaata tttgccagaa ccgttatgat gtcggcgcaa
     6961 aaaacattat ccagaacggg agtgcgcctt gagcgacacg aattatgcag tgatttacga
     7021 cctgcacagc cataccacag cttccgatgg ctgcctgacg ccagaagcat tggtgcaccg
     7081 tgcagtcgag atgcgcgtcg gcaccctggc gatcaccgac catgacacca cagctgcaat
     7141 cgcgccagca agagaggaaa tttcacgttc gggactggcg ttgaatctta ttcccggcgt
     7201 ggaaatttcc acggtctggg aaaatcatga aattcatatt gtcgggctga atattgatat
     7261 tactcatccg ctaatgtgtg agttcctcgc gcagcagaca gaacggcgca atcagcgggc
     7321 gcagctgatt gccgaaagac ttgaaaaagc gcaaatacct ggggcgctgg aaggcgcaca
     7381 acgactggcg cagggcggcg cagtgacgcg cggtcatttt gcgcgtttcc ttgttgagtg
     7441 cggcaaagcc agttcaatgg cggatgtctt taaaaagtat ctggcgcgcg ggaaaaccgg
     7501 atacgttccg ccacagtggt gtacaataga acaagctatt gatgtcattc atcattctgg
     7561 cggtaaggcg gtgctagctc atcctgggcg gtacaatctt agtgctaaat ggctgaaaag
     7621 attggttgcg cattttgccg aacaccacgg tgacgcgatg gaagtcgcgc agtgccagca
     7681 atcgcccaat gaacgtaccc agctggcggc ccttgcgcgt cagcatcatt tatgggcatc
     7741 acaaggatct gattttcatc agccatgccc gtggatcgaa ctgggtcgta aactctggct
     7801 gcccgcaggc gttgaaggcg tctggcagct atgggaacag ccgcagaaca ccacagagag
     7861 ggaattatga gccagttttt ttatattcat cctgataacc cacagcaacg tctgatcaac
     7921 caggcggtgg agatcgtgcg taaaggcggg gtgattgttt atccaactga ttccggctat
     7981 gcgctcggct gtaaaattga agataaaaac gcgatggagc gtatttgtcg tattcgccag
     8041 ctgccggacg gtcacaactt taccctgatg tgtcgcgatc tttctgaact ctcgacctat
     8101 tcatttgttg ataacgttgc gtttcgttta atgaaaaaca acacgccggg caactatacc
     8161 ttcatcttga aggggacgaa ggaagtgcca cgccgtctgt tgcaggaaaa acgcaaaacc
     8221 atcggtatgc gtgtgccttc taacccgatt gcgcaggcgt tgcttgaagc actgggcgaa
     8281 ccgatgcttt ccacttcgct aatgctgcca ggcagcgaat ttaccgaatc cgatcccgaa
     8341 gaaattaaag accgtctgga aaaacaggta gatttgatta ttcacggcgg ctatctcggg
     8401 cagaaaccga caacggttat tgatcttacc gatgataagc cggtcgtggt gcgtgaaggc
     8461 gtaggtgatg tgaagccttt cttataagtg cgtaactctg tatactacgc ggccttaaaa
     8521 tggcgctata cggcgccatt gattctcctg ttcgacgcct gtgaaggcga cactaaaagg
     8581 aagctctatg agcgaaaaat tacagaaagt gctggcccgc gctggccacg gctcccgccg
     8641 tgaaatcgaa tctattattg aagccggtcg tgtgagtgtt gatggcaaaa ttgctaaact
     8701 cggcgatcgc gttgaagtta cccctgggct gaaaattcgt atcgatggtc atctgatttc
     8761 ggtacgtgag tccgctgaac aaatttgtcg cgttctggcc tattacaaac cggaaggtga
     8821 gttgtgtacc cgtaacgatc cggaagggcg tccgacggtg tttgaccgtc tgccaaagct
     8881 gcgtggcgca cgctggattg ccgtaggtcg tctggacgtt aacacctgtg gtctgctgct
     8941 gttcaccacc gatggtgaac tggcaaaccg tttaatgcat ccaagccgtg aagttgaacg
     9001 tgaatacgcc gtgcgtgtat ttggtcaggt tgacgacgcg aaactgcgtg atttgagtcg
     9061 tggcgtgcaa ctggaagatg gtccggcagc ttttaaaacc atcaagttca gcggcggcga
     9121 agggatcaac cagtggtaca acgtgactct gaccgaaggc cgtaaccgtg aagttcgtcg
     9181 tctgtgggaa gcggttggtg tgcaggtgag ccgcctgatc cgtgttcgtt acggtgatat
     9241 cccactgcca aaaggtctgc cacgcggtgg ctggacggag ctggacctcg cccagactaa
     9301 ctatctgcgc gaactggtgg agctaccgcc agaaaccagc tctaaagtcg ctgtagaaaa
     9361 agaccgtcgt cgcatgaagg cgaatcagat tcgtcgtgcg gtgaaacgtc acagtcaggt
     9421 gagcggcggt cgtcgttctg gcggacgtaa taacggttaa tcaaagtatg ccggacgtca
     9481 tatccggcat ttttacagat tagtaatcaa tccctatctg cgcttttaca ccggcatcaa
     9541 acgcatgttt gacggggcgt aattcactta ccgtatctgc cagttcaaga atatcccgat
     9601 gacaaccacg acccgtgata atcaccgtct gttgatgtgg acgttcattt aacgcctgca
     9661 ccacttcttc cagtggcaaa tagtcatacg ccaccatata cgtcagttca tcaagcaaaa
     9721 ccatatccag tgaggaatca gcaagcatcc gctttgcatg ttgccagact tcgcggcagg
     9781 cggcggtatc agactcgcgg ttttgtgtat cccaggtaaa gcccgttgcc atcacctgaa
     9841 actcaacgcc atgtggctcc agcagattgc gttcgccatt aggccaggtg cctttaataa
     9901 actgcacgac acctactttt tttccgtgac caactgcgcg tgttgccgta ccaaatgccg
     9961 cggtggtttt gccttttcca ttgccggtaa agacgatgat aataccgcgt tcatcctggg
    10021 cctgggccac acgagcatct actttttctt tcactcgctg ctgacgctgt tggtagcgtt
    10081 catcactcat tgggaaattc ctggtttacg gcccggttgg gcgtcaaagg tcatgccggt
    10141 tttacggcgg ctgtcatcgc ccatcagcca gaggtagagc ggcatgatat cagcgggtgt
    10201 tttaagtttc tgtggatctt cggtcgggaa ggcgctggca cgcattgcgg tgcgcgtacc
    10261 gcctgggtta atgcagttga cgcgcaggcg ctgctgatat tcatcggcca gtacctgcat
    10321 catcccttcg gtggcaaatt tcgacgctgc atatgcaccc cagttagctc gtccctgacg
    10381 tccaacgctt gatgaagtaa agaccagtga accggcgtcc gatttgagta ataaaggaag
    10441 cagcgcctgg gtgagcaaaa aggtggcatt aacgttgacc tgcatgacgt cctgccagac
    10501 ctgcggattt tgttcgctca ttgggcaaac atcgccgagc aatccggcat tatgcaaaac
    10561 accatccaga cgcggataat taacggtaat gcgctgtgcc agttgttggc aatcttcgga
    10621 cgtgcaggtc agcaaatcga gaataaacca ctgtggctga cgtccagttt cttcgtttat
    10681 gtggctggct acctgacgta atttttcttc attacggccc aacagaatca ctgtcgcacc
    10741 atagcgtgca tacgtcatcg cggcttcacg gccaatccca tcgctggctc ccgtcaccag
    10801 gataatgcga tcattgagta aatcttgttt tggctggtaa tgcatggcta ctcctcaacg
    10861 acgttgtctg tcgcgctgcc agtgtacttt atgactttgg ggctttatgc ctgaaacaga
    10921 agggcatttc aatcagcctg gggcgataac accgcaaaat ggatactttg tcatactttc
    10981 gctgcaataa catctctgcg agacggctta acatgcctgt tgtaaactgt gagccaaagc
    11041 gttgtttaac caaggtgggg actcgtggaa ttgttgtctg aatatggttt gtttttggcg
    11101 aaaatcgtta ccgttgtgct agcgattgcg gcgattgccg ccattattgt caatgttgct
    11161 caacgtaata aacgccagcg tggcgagtta cgggtcaaca atctcagcga acagtataag
    11221 gagatgaaag aagaactggc cgcggcgctg atggactcac atcagcaaaa acagtggcac
    11281 aaagcgcaga agaaaaagca caagcaagaa gcgaaagcag caaaagcgaa agccaaactg
    11341 ggcgaggtgg caactgacag taaacctcgc gtctgggtgc tggattttaa aggcagcatg
    11401 gacgcccatg aagtgaactc gctacgtgaa gagataacgg cggtactcgc agcattcaaa
    11461 ccgcaggatc aggttgtgct ccgtctggaa agccctggtg gcatggtgca tggttacggg
    11521 ttggcggctt cgcagctgca gcgtctgcgt gataaaaaca ttcctttaac tgttacggta
    11581 gacaaagtcg ctgccagcgg cggttacatg atggcctgtg tggcggacaa aattgtttcc
    11641 gcaccgtttg ctattgtggg ttccattggg gtggtggcgc aaatgcccaa ctttaaccgc
    11701 ttcctgaaaa gcaaagatat tgatatcgaa ctgcacactg ccgggcagta taagcgtact
    11761 ctgaccttgc tgggtgaaaa taccgaagaa gggcgggaga aattccgcga agagttgaac
    11821 gaaacgcatc agttgtttaa agattttgtg aagcgtatgc gtccgtctct ggatattgaa
    11881 caggtggcaa cgggtgaaca ctggtacgga caacaggcgg tagagaaagg cctggttgat
    11941 gaaatcaaca ccagtgatga agttattctt agcctgatgg aaggccgtga agtggttaat
    12001 gtacgctata tgcagcgtaa acgactcatt gaccgattca ccggcagcgc ggcagagagc
    12061 gccgatcgat tgttgctacg ctggtggcag cggggtcaaa agccattgat gtaaaagaca
    12121 aacgcgaggc taagacctcg cgttttgctt taatcaacca gatgatattt ttctgaaagc
    12181 acatgggcca ggtgtttgaa catattaaac accgcggtgc ttttggctgt tggcaatcct
    12241 tgttcatcta aaaagtagtc gccggtaaat accagcacgc cattacgctg cgtgacgccg
    12301 gtggcttcaa tccctgcgag cgtatcctca tgctcacgaa tgattttgtt ggcctctttc
    12361 aacagcgttt cgcggtcgat gggttgtgtc tctttgttca ttatttactc cttaaacaag
    12421 gacattagtc tacgccaggc atggcttgca gacaaatata ccacgctggt ggcaagagcg
    12481 ccttactggt aactttggat tttgcatgct aataaagttg cgtatcggat tttatcaggt
    12541 acagtgtgac gctttcgtca atctggcaat agatttgctt gacattcgac caaaattccg
    12601 tcgtgctata gcgcctgtag gccaagacct gttaactcag tcacctgaat tttcgtgaac
    12661 agagtcacga caaggggttg atatccgcag agaacgagtc catatcggta actcgttgcc
    12721 agtggaaggt ttatcaacgt gcgacgcatt cctggaagaa tcaaattagg taaggtgaat
    12781 atgggtaaag ctcttgtcat cgttgagtcc ccggcaaaag ccaaaacgat caacaagtat
    12841 ctgggtagtg actacgtggt gaaatccagc gtcggtcaca tccgcgattt gccgaccagt
    12901 ggctcagctg ccaaaaagag tgccgactct acctccacca agacggctaa aaagcctaaa
    12961 aaggatgaac gtggcgctct cgtcaaccgt atgggggttg acccgtggca caattgggag
    13021 gcgcactatg aagtgttgcc tggtaaagag aaggtcgtct ctgaactgaa acaactggct
    13081 gaaaaagccg accacatcta tctcgcaacc gaccttgacc gcgaagggga agccattgca
    13141 tggcacctgc gggaagtgat tgggggtgat gatgcgcgct atagccgagt ggtgtttaac
    13201 gaaattacta aaaacgcgat ccgccaggca tttaataaac cgggtgagct gaatattgat
    13261 cgtgttaatg cccagcaggc gcgtcgcttt atggaccgcg tggtggggta tatggtttcg
    13321 ccgctgctat ggaaaaagat cgctcgtggt ctgtctgccg gtcgtgtgca gtcggtggcg
    13381 gttcgcctgg tggtcgagcg tgagcgtgaa attaaagcgt tcgtgccgga agagttctgg
    13441 gaagtcgatg ccagcacgac cacgccatct ggtgaagcgt tggcgttaca ggtgactcat
    13501 cagaacgaca aaccgttccg tccggtcaac aaagaacaaa ctcaggctgc ggtaagtctg
    13561 ctggaaaaag cgcgctacag cgtgctggaa cgtgaagaca aaccgacaac cagtaaacct
    13621 ggcgctcctt ttattacctc tacgctgcaa caagctgcca gcacccgtct tggatttggc
    13681 gtgaaaaaaa ccatgatgat ggcgcagcgt ttgtatgaag caggctatat cacttacatg
    13741 cgtaccgact ccactaacct gagtcaggac gcggtaaata tggttcgcgg ttatatcagc
    13801 gataattttg gtaagaaata tctgccggaa agtccgaatc agtacgccag caaagaaaac
    13861 tcacaggaag cgcacgaagc gattcgtcct tctgacgtca atgtgatggc ggaatcgctg
    13921 aaggatatgg aagcagatgc gcagaaactg taccagttaa tctggcgtca gttcgtagcc
    13981 tgccagatga ccccagcgaa atatgactcc acgacgctga ccgttggtgc gggcgatttc
    14041 cgcctgaaag cacgcggtcg tattttgcgt tttgatggct ggacaaaagt gatgcctgcg
    14101 ttgcgtaaag gcgatgaaga tcgcatctta ccagcagtta ataaaggcga tgctctgacg
    14161 ctcgttgaac ttacaccagc ccagcacttt accaagccgc cagcccgttt cagtgaagca
    14221 tcgctggtta aagagctgga aaaacgtggt atcggtcgtc cgtctactta tgcgtcgatc
    14281 atttcgacca ttcaggatcg tggctacgtg cgagtagaaa atcgtcgttt ctatgcggaa
    14341 aaaatgggcg aaatcgtcac cgatcgcctt gaagaaaatt tccgcgagtt aatgaactac
    14401 gactttaccg cgcagatgga aaacagcctc gaccaggtgg caaatcacga agcagagtgg
    14461 aaagctgtac tggatcactt cttctcggat ttcacccagc agttagataa agctgaaaaa
    14521 gatccggaag agggtggtat gcgcccgaac cagatggttc tgaccagcat tgactgcccg
    14581 acttgtggtc gcaaaatggg gattcgcaca gcgagcaccg gggtattcct tggctgttct
    14641 ggctatgcgc tgccgccgaa agagcgttgc aaaaccacca ttaacctggt gccggaaaac
    14701 gaagtgctga acgtgctgga aggcgaagat gctgaaacca acgcgctgcg cgcaaaacgt
    14761 cgttgcccga aatgcggcac ggcgatggac agctatctca tcgatccgaa acgtaagttg
    14821 catgtctgtg gtaataaccc aacctgcgac ggttacgaga tcgaagaggg cgaattccgc
    14881 attaaaggtt atgacggccc gatcgttgag tgtgaaaaat gtggctctga aatgcacctg
    14941 aaaatggggc gtttcggtaa atacatggcc tgcaccaacg aagagtgtaa aaacacacgt
    15001 aagattttac gtaacggcga agtggcacca ccgaaagaag atccggtgcc attacctgag
    15061 ctgccgtgcg aaaaatcaga tgcttatttc gtgctgcgtg acggtgctgc cggtgtgttc
    15121 ctggctgcca acactttccc gaaatcgcgt gaaacgcgtg cgccactggt ggaagagctt
    15181 tatcgcttcc gcgaccgtct gccggaaaaa ctgcgttatc tggccgatgc gccacagcag
    15241 gatccggaag gtaataagac catggttcgc tttagccgta aaaccaaaca gcaatatgtc
    15301 tcttcggaaa aagacggaaa ggcgactggc tggtcagcat tttatgttga tggcaaatgg
    15361 gttgaaggaa aaaaataacc tttaattctg tcaggttttt ataaacaaag ggtcgcgaaa
    15421 gcggcccttt tttattgcat attatttttt cttcacacct atacactaag gctataaatg
    15481 atatagtggt tatagttagc acctttttta ttattaaatc gtattagtca cccgccaggt
    15541 gtgacgaaaa aacgatgttc tgatggcgtc taagtggatg gtttaacatg aaattacaac
    15601 aacttcgcta tattgttgag gtggtcaatc ataacctgaa tgtctcatca acagcggaag
    15661 gactttacac atcacaaccc gggatcagta aacaagtcag aatgctggaa gacgagctag
    15721 gcattcaaat tttttcccga agcggcaagc acctgacgca ggtaacgcca gcaggacaag
    15781 aaataattcg tatcgctcgc gaagtcctgt cgaaagtcga tgccataaaa tcggttgccg
    15841 gtgagcacac ctggccggat aaaggctcgc tgtatatcgc caccacgcat acccaggcac
    15901 gctacgcgtt accaaacgtt atcaaaggct ttattgagcg ttatccccgc gtttctttgc
    15961 atatgcacca gggctcgccg acacaaattg ctgatgccgt ctctaaaggc aatgctgatt
    16021 tcgctatcgc cacagaagcg ctgcatctgt atgaagattt agtgatgtta ccgtgctacc
    16081 actggaatcg ggctattgta gtcactccgg atcacccgct ggcaggcaaa aaagccatta
    16141 ccattgaaga actggcgcaa tatccgttgg tgacatatac cttcggcttt accggacgtt
    16201 cagaactgga tactgccttt aaccgcgcag ggttaacgcc gcgtatcgtc ttcacggcaa
    16261 cggatgctga cgtcattaaa acttacgtcc ggttagggct gggggtaggg gtcattgcca
    16321 gcatggcggt ggatccggtc gccgatcccg accttgtgcg tgttgatgct cacgatatct
    16381 tcagccacag tacaaccaaa attggttttc gccgtagtac tttcttgcgc agttatatgt
    16441 atgatttcat tcagcgtttt gcaccgcatt taacgcgtga tgtcgttgat gcggctgtcg
    16501 cattgcgctc taatgaagaa attgaggtca tgtttaaaga tataaaactg ccggaaaaat
    16561 aatttcttgc gttattttcg gcacctttta tgtagcgaag gtgccggaat atattctctt
    16621 ttgttactta tttctatacc gtgcacaata tttttatccc tcatatttat agggtaaatt
    16681 acctgtaaag tagtgtctaa ttacccgcct cgcaaactcc agttttcaaa tatctatttc
    16741 aggtcaaaag attgaattat tcactggaga cgattcgtaa attcgctgga tttttgacta
    16801 aagtttcttt aggatttgtc tcatcgtatg attaattaca ccaatcgttc aacgtcttgc
    16861 tgataagtga tggcgattat atgaggttag caatgccttc tggaaatcag gaaccccgcc
    16921 gcgatcctga attaaaacgt aaagcctggc tggcggtttt tcttggttct gcacttttct
    16981 gggtggttgt cgcactgctg atttggaaag tgtggggata actatggtcg gtcaggagca
    17041 actggagtcg tcaccattat gccagcatag tgacaatgaa acagaaacga aacgggaatg
    17101 ttccgtcgtt attccagacg actggcaact aacatcgcag cagcaagcct ttatagaact
    17161 gtttgctgaa gatgatcagc cgaaacaata attatcatca ttcttattac ccatttttaa
    17221 tgaattaaag ggcttttaat acaccgcagc aataacagct tgagttatct caacacaaaa
    17281 taataaccgt taagggtgta gcctatgatc aacacaaata tgaaatattg gtcctggatg
    17341 ggcgcgtttt ctctgtcgat gctcttctgg gccgaactcc tctggatcat tactcactga
    17401 tccttgaccc cgctgcggcg gggttgtcat ttgctttgcc acaaggtttc tcctctttta
    17461 tcaatttggg ttgttatcaa atcgttacgc gatgtttgtg ttatctttaa tattcaccct
    17521 gaagagaatc agggcttcgc aaccctgtca ttaaggagga gctatgtcgt caaccctacg
    17581 agaagccagt aaggacacgt tgcaggccaa agataaaact taccactact acagcctgcc
    17641 gcttgctgct aaatcactgg gcgatatcac ccgtctaccc aagtcactca aagttttgct
    17701 cgaaaacctg ctgcgctggc aggatggtaa ctcggttacc gaagaggata tccacgcgct
    17761 ggcaggatgg ctgaaaaatg cccatgctga ccgtgaaatt gcctaccgcc cggcaagggt
    17821 gctgatgcag gactttaccg gcgtacctgc cgttgttgat ctggcggcaa tgcgcgaagc
    17881 ggttaaacgc ctcggcggcg atactgcaaa ggttaacccg ctctcaccgg tcgacctggt
    17941 cattgaccac tcggtgaccg tcgatcgttt tggtgatgat gaggcatttg aagaaaacgt
    18001 acgcctggaa atggagcgca accacgaacg ttatgtgttc ctgaaatggg gaaagcaagc
    18061 gttcagtcgg tttagcgtcg tgccgccagg cacaggcatt tgccatcagg ttaacctcga
    18121 atatctcggc aaagcagtgt ggagtgaatt gcaggacggt gaatggattg cttatccgga
    18181 tacactcgtt ggtactgact cgcacaccac
//
LOCUS       DAZGLJ010000084        18096 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000084 DAZGLJ010000000
VERSION     DAZGLJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.084"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..191
                     /locus_tag="ACHK49_002493"
     CDS             <1..191
                     /locus_tag="ACHK49_002493"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263127.1"
                     /translation="FSRPQKTNKAAKWPLYSHHQKLYFTLIFILFSLIFSWTFPVFTD
                     DISRYLARCGCAPTHPHR"
     gene            223..564
                     /gene="symE"
                     /locus_tag="ACHK49_002494"
     CDS             223..564
                     /gene="symE"
                     /locus_tag="ACHK49_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO9263128.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPTAEESELMQSLRQVCKLSARKQKQVQDF
                     IGVITGKQKVA"
     gene            complement(611..1774)
                     /locus_tag="ACHK49_002495"
     CDS             complement(611..1774)
                     /locus_tag="ACHK49_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HFO9263129.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNDDLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFMNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRVHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIRARADRLADLAVQVWQAPSLSEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GSHSRVLFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(1822..2704)
                     /locus_tag="ACHK49_002496"
                     /pseudo
     CDS             complement(1822..2704)
                     /locus_tag="ACHK49_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(2801..2965)
                     /gene="yjiS"
                     /locus_tag="ACHK49_002497"
     CDS             complement(2801..2965)
                     /gene="yjiS"
                     /locus_tag="ACHK49_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO9263130.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            3142..4554
                     /gene="yjiR"
                     /locus_tag="ACHK49_002498"
     CDS             3142..4554
                     /gene="yjiR"
                     /locus_tag="ACHK49_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO9263131.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSREHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWGAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQNYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(4796..5740)
                     /locus_tag="ACHK49_002499"
     CDS             complement(4796..5740)
                     /locus_tag="ACHK49_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO9263132.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIYLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVNTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRALPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKKFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            6207..7439
                     /gene="mdtM"
                     /locus_tag="ACHK49_002500"
     CDS             6207..7439
                     /gene="mdtM"
                     /locus_tag="ACHK49_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO9263133.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVHQHQAAELAEER"
     gene            7480..8760
                     /gene="yjiN"
                     /locus_tag="ACHK49_002501"
     CDS             7480..8760
                     /gene="yjiN"
                     /locus_tag="ACHK49_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO9263134.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            8876..10027
                     /gene="yjiM"
                     /locus_tag="ACHK49_002502"
     CDS             8876..10027
                     /gene="yjiM"
                     /locus_tag="ACHK49_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO9263135.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKAVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            10037..10804
                     /gene="yjiL"
                     /locus_tag="ACHK49_002503"
     CDS             10037..10804
                     /gene="yjiL"
                     /locus_tag="ACHK49_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418754.2"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO9263136.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEAISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            10801..11058
                     /gene="ytiA"
                     /locus_tag="ACHK49_002504"
     CDS             10801..11058
                     /gene="ytiA"
                     /locus_tag="ACHK49_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO9263137.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEMQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            11123..11983
                     /gene="yjiK"
                     /locus_tag="ACHK49_002505"
                     /pseudo
     CDS             11123..11983
                     /gene="yjiK"
                     /locus_tag="ACHK49_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
     gene            12051..13229
                     /gene="yjiJ"
                     /locus_tag="ACHK49_002506"
     CDS             12051..13229
                     /gene="yjiJ"
                     /locus_tag="ACHK49_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9263138.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(13242..13796)
                     /gene="kptA"
                     /locus_tag="ACHK49_002507"
     CDS             complement(13242..13796)
                     /gene="kptA"
                     /locus_tag="ACHK49_002507"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO9263139.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRTLLDTVVATSDKKRFSYSSDGRCIRAVQGHLTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQTENGVWLTSTVAVEFLEW"
     gene            14046..14729
                     /gene="yjiH"
                     /locus_tag="ACHK49_002508"
     CDS             14046..14729
                     /gene="yjiH"
                     /locus_tag="ACHK49_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO9263140.1"
                     /translation="MTQQGDAVAGDLATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            14726..15187
                     /gene="yjiG"
                     /locus_tag="ACHK49_002509"
     CDS             14726..15187
                     /gene="yjiG"
                     /locus_tag="ACHK49_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO9263141.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            15200..16372
                     /gene="iadA"
                     /locus_tag="ACHK49_002510"
     CDS             15200..16372
                     /gene="iadA"
                     /locus_tag="ACHK49_002510"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO9263142.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILFPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            16437..17349
                     /gene="hypT"
                     /locus_tag="ACHK49_002511"
                     /gene_synonym="qseD"
                     /pseudo
     CDS             16437..17349
                     /gene="hypT"
                     /locus_tag="ACHK49_002511"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
     gene            complement(17342..17734)
                     /gene="iraD"
                     /locus_tag="ACHK49_002512"
     CDS             complement(17342..17734)
                     /gene="iraD"
                     /locus_tag="ACHK49_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO9263143.1"
                     /translation="MMRQSRQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSSLPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHTLAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(17731..17814)
                     /gene="idlP"
                     /locus_tag="ACHK49_002513"
     CDS             complement(17731..17814)
                     /gene="idlP"
                     /locus_tag="ACHK49_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO9263144.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(17930..18073)
                     /gene="ytiD"
                     /locus_tag="ACHK49_002514"
     CDS             complement(17930..18073)
                     /gene="ytiD"
                     /locus_tag="ACHK49_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFO9263145.1"
                     /translation="MADYAEINNFTPELSSGGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
BASE COUNT         4245 a         4572 c         4505 g         4774 t
ORIGIN      
        1 aattttctcg ccctcaaaaa accaataaag cggctaaatg gccgctttat tcacatcacc
       61 aaaaattata tttcacgcta attttcatct tattttcatt aatttttagc tggactttcc
      121 ctgtatttac tgatgatata tccaggtatt tagcgcggtg cggatgtgcg ccaacacacc
      181 cgcaccgcta atcacaatca ctattatcgg agaatagcag ttatgactga cacgcattct
      241 attgcacaac cgttcgaagc agaagtctcc ccggcaaata accgtcaatt aaccgtgagt
      301 tatgcgagtc gctacccgga ttacagccgt attcccgcca tcaccctgaa aggtcagtgg
      361 ctggaagccg ccggttttgc caccggcacg gcggtagatg tcaaagtaat ggaaggctgt
      421 attgtcctca ctgcccaacc acccaccgcc gaagagagcg aactgatgca gtcgctgcgc
      481 caggtgtgca agctgtcggc gcgtaaacaa aagcaggtgc aggattttat tggggtaatt
      541 accggtaaac agaaagtcgc ctgataaatt gacccggtag cgccagtgct accgggtatt
      601 attcgccgcg ttataccaac gcgctctcca tctgtttttc aaatgcctga cgaattaatc
      661 ccataatgta aggaagttgt gcgaggtcgc tgaaaccaat ttctacatcg ccattacccc
      721 agcgcccaac atttgtcaca tctttggcaa tacctttcgg atcgaccagt tcatgaaact
      781 gcatattaag cgacaaccgc agtcggcttt tttgcggtac aacatcaaca aaattcgttt
      841 cagctttaaa cgcaatatag agcttcaata cttcctgcgt gatccctgcg tccaggcgca
      901 taacttcatc gcgaagatga tcgaataaca cccggctatg cgaaccatca gcaagaaaag
      961 gataatcact caggctgtaa ctggttttgt tttcaggctg cccccgataa acagctaaaa
     1021 cctcttcaga aagagaaggc gcttgccaga cctgaaccgc gagatcggcc agacgatcgg
     1081 cacgggcgcg aatggcggct tcatcccatt tttcgcactg cccaagaccg atattcaaat
     1141 agagcgggct atgtttaaag ccgtcttcaa tatcgcgttt ttccgcgaaa ggtctgtcac
     1201 tgtagcggga gttatagcgc gttaaagtga gattccccaa cgtatgtaac agttctttat
     1261 gcacacgctg ccagtcgctt cccagctctt cacgccattt agccgacaga ttttcgttct
     1321 gcggcataat gtgctcaatc gtaaactctt ccacgcgctc tctgcgttta tcgttttcca
     1381 gtcttcgtaa ccagtagctg cggctgcgga agttatagag atcgcgaact tttaattccc
     1441 gtttaaattc atcatcgttg gggaaacgac gatatgaagg caggttcata aaatgtacct
     1501 gaatactttc cagatatttt tctttattaa tgaccttata aaaagtggca aacgtcttgt
     1561 tcaaagaatt cgtcggaatt gcacatactg cacggcggaa aacataagat tcaattaaac
     1621 gaattatgct caggaaatct tcgtgagaca aatcgtcatt tttatagtca tgataaagcg
     1681 ccagtaagaa aggatacgcc acatcaacct ttaactcgcg caaatcctga aaagccgtag
     1741 caagcgattt gtcactttct tttcccaatg ccattgcaca gtaatactcc gcgtagatat
     1801 gaatatcttc aaccagccga tctacgcctt tttcagcaac actctggctg cgggcatgga
     1861 gtttaaatgc ctcatagact tcatctgtcc gagggatctc ccccgttttt accgtcagat
     1921 aatgacgcat aaaactgtca aaatattcgc tgtaaccttg ctgaccaaag gcgacttcca
     1981 ttggacgcca gtgatcttca tacaaccggg tttgatgctc tggttcgagg cccatcagaa
     2041 taaaattgcg caccagatcg gcctgactta acgccttacc ggtggagttc atactttcaa
     2101 aaatcagttg cggattatcc tgaccacgat taagcgccac atcgacaatt aataactttg
     2161 ctaacccacg acataagggg atcaagtcat caccgagttt ggcaatttgt tcatcaaaga
     2221 aagtaaagtt ttccattatt cgatgggaat agttttctgg cattggtctt tgttttatta
     2281 acgccagtaa actgtcttta tcagtctcgg tcagtagtaa tttaaaacct ttctcgccgg
     2341 actcataagg attgagcaaa taataattac gcaatttcat tgctgaaaag ccatcaaaaa
     2401 cttcgtcttc gccaagatgg cgcgataaag cctcaatcag caacattgcg gtcgtcagcc
     2461 gttgttgacc atcaatgacc agtaacggag aaataccaga aacctgatac aacccctgct
     2521 caatataaac aaccgaaccg ataaaatgcg ctgatatatc gtcacgcttt ccggcacgaa
     2581 tgatgtcgtc ccaaagttgc cgacattgtt gttctgtcca tgaataagtc cgttgataaa
     2641 tgggaataac aaactgctgc gaacgtttta aaaaatcgag taggcgggct tctgtagctt
     2701 tcattctgac gtctttccgt gaagcgatga tttcgcaata ataaccactt ccttttggag
     2761 gttatagaga aatcctgttt atccgcgatg aattgccccc tcactccaca tcctccctgc
     2821 gtaacccaat atctctcaac cgctcatcac tcatctgctg taacactcgt cgggtctgca
     2881 tttgccgccg ccaacgcctc accgcctgcc agagttgcac aagtctgata aacggcgttt
     2941 tggctttgtt ttcgtgaaat tccatctgct gcctcctcat ctttcagagg cttcatcatc
     3001 caccttctgg cagttcacaa tacagatgca aatattagtt ttatttaaca tacagacagg
     3061 ttaaaacagc atctgcatcc ggttttttag cctcatctgt accggttttt ctttctgtat
     3121 ggtccaaata aggaatacag catgacgcgt tatcaacatc tggcgactct acttgccgag
     3181 cggattgagc aagggctgta tcgtcatggg gagaaattgc cgtcggtgcg tagcttaagt
     3241 cgggagcacg gcgtcagcat cagcaccgtg cagcaggcgt atcagacgct ggagacgatg
     3301 aagctcataa ccccacagcc gcgttcgggt tattttgtcg cacaacgtaa agcccagccg
     3361 ccagtgccgc cgatgacgcg tccggtgcag cgcccggtgg aaattaccca gtgggatcag
     3421 gtgctggata tgctggtggc gcatagcgac agttccattg ttccgttaag caaaagcacg
     3481 ccggatgtcg aaacgcccag cctgaaaccg ctctggcggg agctaagccg ggtggtgcag
     3541 cataatctgc aaaccgtgct cggttatgac ttgttagccg gtcagcgggt attgagagag
     3601 cagattgccc gcctgatgct cgacagcggc tcggtggtca ccgccgatga catcattatc
     3661 accagcggct gccataactc gatgtcgctg gcgttaatgg cggtgtgtaa accgggcgat
     3721 attgtcgcgg tcgaatctcc ctgttattac ggttcgatgc agatgctgcg cggcatgggc
     3781 gtgaaagtga ttgaaatccc aaccgatcca gaaacaggca tcagcgttga agcgctggaa
     3841 ctggcgctgg aacagtggcc gattaaaggc atcattctgg tgccaaactg taataatccg
     3901 ctgggattta ttatgccgga cgcacgcaaa cgggccgttc tctctctcgc tcagcgtcat
     3961 gatattgtga tttttgaaga tgatgtctac ggcgaactgg caacggagta tccgcgcccg
     4021 cggaccatcc attcatggga tatcgacggg cgagtgctgt tgtgcagctc gttcagtaaa
     4081 agtattgctc caggcctgcg cgtgggttgg ggcgcaccgg ggcgttatca cgataaactg
     4141 atgcatatga aatacgccat cagcagcttt aatgtgccgt ccacgcaaat ggcggcggca
     4201 acgtttgtgc tggaaggtca ctatcatcgc catatccggc ggatgcggca gaactatcag
     4261 cgcaatttgg cgctttatac ctgctggata cgggaatatt ttccctgcga aatctgtatt
     4321 acgcgcccga aaggcggatt tttactgtgg atagaattgc ctgaacaggt cgatatggtc
     4381 tgcgttgcgc ggcagctgtg ccgcatgaat atccaggtgg cagcaggctc gattttctcg
     4441 gcttccggca aataccgtaa ttgtctacgc atcaactgcg ctttgccgct cagcgaaacc
     4501 tatcgcgaag cactaaagca aattggcgag gccgtgtatc gggcaatgga ataaattcga
     4561 attagtggcg aagcacctga tttcctctca cgaatcgcct gtaactcgtt gtatctttgc
     4621 agggtcatcg ccacgttcca gcatggctta ggcaaagtgt gcttcgttca taccggatgc
     4681 ggcgtgaacg ccttatccgg cctacaaacc gcgcaaattc aatagattgc agaggaaatg
     4741 taggtctgat aagcgtagcg catcaggcag ctttgcgttt gtcatccgtc tctgattagt
     4801 attcgatcca tggatcgcgc ttgatttcag gcaagggctg ctctaatgcc tgcatttgct
     4861 ctgtcgaaag tccggtaatc ttttgtatcc attcaggatc tgccccattc tgcaacaaca
     4921 atctaaggag gcgttgttcc ccttcttctt tccccttttc cattcccaac agccatccat
     4981 cattataaat tcgctctgca atcgtcatta ttcgctccct gtgttgtggc attcgacggg
     5041 taagttcgct gataaacttc ttaaaacgcg cttcatcgcc agtcagtaaa atgtaattta
     5101 acagcgcagt tatctggctg tcattagcac actctgtaac cagtaatact accagttggt
     5161 cgataagccc catcagatcg cgctggcgaa tatgcttttg gatcaactcc aacagggcga
     5221 ctctgcgatg ctgcacaatc tcgtcgtctg gcacgacagt gatatccacc agcgggaacg
     5281 cggcggtata aagtttccgt gcggtagttg ggtcggcaaa ttcgtccagc cagcacaggg
     5341 accagggata aggactacgg ctaccgtgat aaaatagcat cggtataacc agcggtagcg
     5401 cccgacgttt atcatgctcg atatggcgct gcatcaccgc catggaatag cgcatcaggc
     5461 gaaaggccat atgaatatcc tcacggctct gatgttcaat caccacataa atatagccat
     5521 cgccttcgcg ggtatttacc gaccacagaa tatcggagtg tagcgcccgc aatttttcat
     5581 cgacaaagct ggcggattcc agttttaagc tgtcgagatc gcacagttca cgtaaatctt
     5641 tgggtaaata gatctccata aaatcccgcg cggtgtcagg gtgggtgaga aaggatttaa
     5701 ataatgcgtc atgcggcgtg ctggttgtga agtttgtcat ggcgatccgt cacctgttga
     5761 taagatggcc cgactttagc gactaacaaa accattcaca cctggcaatt tcccgttccc
     5821 cgaggcgctt tccaggctat ttttgttcct ctgcaagcga tacatttttc cggaaacagg
     5881 ctcgcgtttt acatttcctg tacccggtag atattcccct ttccaccgcg ctcttttgat
     5941 taatcgcaaa ttttaattac acttaaggtg tatattttct atgcaaacca tcaattcaag
     6001 aggtgtaatg tgctgatgac tatttgtaat cgttatacat tctgacccga actcagaagg
     6061 tatttctctg tctgtataag cacaggcagt gtggttgatt acatgaaatt cagtacattt
     6121 gtccttctcg cctacctttc gtcattaccg acggtattga atttcgtttt ccccgttggg
     6181 gttctccgga caaggagttg tttgttatgc cacgtttttt tgcccgccat gccgccacgc
     6241 tgtttttccc gatggcgttg attttgtatg attttgccgc gtacctgtcg acggacctga
     6301 ttcagccggg gatcattaat gtggtccgtg attttaatgc cgatgtcagt ctggccccgg
     6361 cagccgtcag tctctatctc gcgggcggta tggcgttaca gtggctgctg gggccgcttt
     6421 ccgacaggat tggccgcagg ccggtgctga ttaccggggc gctaatattt acccttgcct
     6481 gcgctgcgac aatgttcaca acgtctatga cacagtttct tatcgcgcgt gcaattcagg
     6541 gcaccagtat ctgttttatt gctaccgttg gttatgtcac ggtgcaggag gcgttcggac
     6601 agacaaaagg gatcaagttg atggcgatta tcacctccat cgtactgatt gcgccgatta
     6661 ttggcccgct ttccggcgca gctctgatgc actttgtgca ctggaaagtc ctttttgcca
     6721 tcattgcggt tatgggtttt atctcatttg ttggcttact gctggcgatg ccagagacgg
     6781 tgaagcgcgg cgcggttcca tttagcgcca aaagcgtctt gcgcgatttt cgtaatgtct
     6841 tttgcaatcg gctgttcctc tttggcgcag caaccatctc tttaagctat atcccaatga
     6901 tgagttgggt ggctgtctcg ccggtgatcc tgattgacgc aggcggctta acaacttcgc
     6961 agttcgcctg gacacaggtt ccggtgttcg gcgcggtgat tgtcgcgaat gccatcgtgg
     7021 cgcgtttcgt taaagatccg accgaaccgc gctttatctg gcgtgccgta cccattcaac
     7081 tggtcggcct cgcactgttg attatcggca atctgctgtc accgcacgtc tggctgtggt
     7141 cggtgctggg caccagtctg tatgctttcg ggattggttt aatcttcccg accttattcc
     7201 gctttacact tttttccaat aacttaccga aagggaccgt ctccgcatcg ctgaatatgg
     7261 tgatcctgat ggtgatgtct gtctcggtcg aaatcggccg ctggctgtgg tttaacggcg
     7321 gtcgcttgcc gtttcatctg ttagccgtcg tagcgggagt tatcgtcgtt ttcaccctgg
     7381 cggggttgct caatcgcgtg caccaacatc aggctgccga gctggcggag gagcggtgat
     7441 ttttgcgcga tcctgccgtc aggctctatt cttaacgtta tgaataaact cattgaactc
     7501 agacgcgcca aaatgttggc gctctcttta ctgcttatcg ccgctgctac ctttgtcgtt
     7561 acgctgtttt tgccacccaa tttttgggtg agcggcgtga aggcaattgc tgaagcggcg
     7621 atggtcggcg cgctggcgga ctggtttgcg gtggtggcgc tgtttcgccg cgtgccaatt
     7681 ccgatcattt ctcgtcatac ggcgattatc ccgcgtaata aagaccggat tggcgaaaat
     7741 ctcggtcagt tcgtgcagga aaaatttctc gatacccagt cgctggtggc attgattcga
     7801 cgtcacgaac cggcgttgct aattggtaac tggtttagcc agccagaaaa cgcccgccgc
     7861 gttggtcagc atctgttgca gatcatgagt ggttttcttg aactgaccga tgatgcacgt
     7921 attcagcgcc tgcttaagcg cgcggtccat cgggcgattg ataaggtcga tctttccgga
     7981 accagtgcgt tgatgctgga gagtatgacc aaaaacgatc gtcatcaggt gctgctcgat
     8041 acgctgatcg cacagttgat tgctcttctc cagcgcgata aatcgcgcaa gtttatcgcc
     8101 caacaaattg ttcgctggct ggagagtgag catccactga aagccaaaat tctccccacc
     8161 gaatggctgg gcgaacatag cgcggagttg gtttctgacg cggtgaattc tttgcttgat
     8221 gatatcagcc gcgatcgtgc gcatcagatc cgccatgcat ttgatcgcgc cacttttgcc
     8281 ctgatcgaca agttgaaaaa cgatccggaa atggcagcgc gagctgatgc cgtaaaaagc
     8341 tatctgaaag aggatgaagc ctttaaccgc tatctcagtg aattgtgggg ggatttacgg
     8401 gaatggctga aagcggatat caacagtgaa gattctcgtg tgaaagaacg tatcgcgcgg
     8461 gcgggtcaat ggtttggcga aacgttaatt gccgatgatg ccttgcgggc gtcgttaaat
     8521 ggtcatctgg aacaagccgc gcaccgcgtc gcacctgagt tttccgcatt cctgacgcgc
     8581 cacatcagcg atacggtaaa aagctgggat gcccgggata tgtcgcggca aatagagtta
     8641 aatatcggca aagatctgca gtttatccgt gtcaacggta cgctggttgg cggttgtatt
     8701 gggctaattt tatatttgct gtcacagctc ccggccttgt tccccctcgg caatttttag
     8761 aaatccatga aaaactaagt ggctaaccgc gagagagatc aaataaacca cactaaaaga
     8821 atggtaataa tgtggcctct tttcattatc tcccgtggta cggggaagga aaatcatgtc
     8881 acttgtcacc gatctacccg ccattttcga tcagttctct gaagctcgcc agaaaggctt
     8941 tctcaccgtc atggatctca aggagcgcgg cattccgctg gttggcactt actgcacttt
     9001 tatgccgcaa gagatcccga tggcagccgg tgcggttgtg gtttcgctct gttccacctc
     9061 tgatgaaacc attgaagaag cggagaaaga tctgccgcgc aacctctgcc cgctgattaa
     9121 aagcagctac ggcttcggta aaaccgataa atgcccctac ttctactttt ctgatctggt
     9181 ggtcggtgaa accacctgcg acggcaaaaa gaaaatgtat gaatacatgg cggagtttaa
     9241 gcctgttcat gtgatgcagc tgccaaatag cgttaaagac gatgcctcgc gtgcgttatg
     9301 gaaagccgag atgctgcgct tacaaaaagc ggtggaagaa cgttttgggc acgaaattag
     9361 cgaagatgct ctgcgcgatg ccattgctct gaaaaaccgc gaacgtcgcg cactggctaa
     9421 tttttatcat cttgggcagt taaatcctcc ggcgcttagc ggcagcgaca ttctgaaagt
     9481 ggtttacggc gcaaccttcc ggttcgataa agaggcgttg atcaatgaac tggacgcgat
     9541 gaccgcccgc gttcgtcagc agtgggaaga aggccagcga ctggacccgc gtccacgcat
     9601 tttaatcacc ggctgcccga ttggcggcgc agcagaaaaa gtggtgcgcg cgattgaaga
     9661 gaatggcggc tgggttgtcg gttatgaaaa ctgcaccggg gcgaaagcga ccgagcaatg
     9721 cgtggcagaa acgggcgatg tctacgacgc gctggcggat aaatatctgg cgattggctg
     9781 ctcctgtgtt tcgccgaacg atcagcgcct gcaaatgctc agccagatgg tggaagaata
     9841 tcaggtcgat ggcgtagttg atgtgatttt gcaggcgtgc catacctacg cggtggaatc
     9901 gctggcgatt aaacgtcatg tgcgtcagca gcacaacatt ccttatatcg ctattgaaac
     9961 agactactcc acctcagatg tcgggcagct cagtacccgt gtcgcggcct ttattgagat
    10021 gctgtaagga gtggcagtga catattcgat tggcattgat tctggttcta ccgccaccaa
    10081 agggatctta ctggcagacg gcgtgattac gcgccgtttc ctcgttccaa caccctttcg
    10141 tccggcaaca gcaattactg aagcctggga aactctgcgc gaagggttag agacaacgcc
    10201 gtttctgacg ctcaccggct atggacggca actggtggat tttgccgata aacaagtgac
    10261 ggaaatctcc tgtcatgggc tgggcgcacg gtttcttgcg ccagcaacgc gcgcggtaat
    10321 cgacatcggt ggtcaggaca gcaaagtgat tcagcttgat gatgacggta acctgtgcga
    10381 tttcctgatg aatgacaaat gcgcggcggg caccgggcgt ttcctggagg tgatctcgcg
    10441 cacgcttggc accagcgtcg agcaactcga cagcattacc gaaaatgtca cgccgcacgc
    10501 catcacgagt atgtgcacag tgtttgctga atcagaagcg atcagcctgc gctcagcggg
    10561 cgtcgcgcca gaagcgattc tcgccggagt gattaacgcg atggcgcgga ggagtgccaa
    10621 tttcattgct cgtctctcct gtgaagcgcc gattctgttt actggtggcg ttagtcattg
    10681 ccagacgttt gcccggatgc tggaatctca tctgggaatg ccggtaaata cccatcctga
    10741 tgcgcaattt gctggcgcaa ttggcgcggc ggtaattggt caacgagtga ggacacgccg
    10801 atgaaagagt ttttattttt atttcactcc acagtcggcg tcatacaaac ccgcaaagcg
    10861 ttgcaggcag cgggcatgac ttttcgcgtc agcgatattc cccgtgattt acgcggcggc
    10921 tgcgggttat gcatttggct gacctgtccc cccggcgagg aaatgcaatg ggtgatccct
    10981 gggctgactg agtcaattta ttgtcagcag gatggtgtct ggcgctgcat tgcacattac
    11041 cgggtttctc cgcgttaagg attcattaag gtcgctacgt atacttctcg atatcttatt
    11101 gatttgcctt aaagggatta ccgtgacaaa aagtatttcg ttaagtaaac gcatctccgt
    11161 tattgtgatt ctttttgcca ttgtggcagt gtgtactttc ttcgtgcaat cttgcgcccg
    11221 taaaagcaat catgcagcat aatttcagaa ttaccacgcc accattgacg gcaaagaaat
    11281 tgcaggcata acgaataata tctcttcgct gacctggtcg gcacaaagca atactctttt
    11341 tagcactatc aacaaacctg ccgccattgt tgaaatgacc accaatggtg attttatccg
    11401 cacaattcct ctggattttg ttaaggatct cgagacgatc gaatacattg gcgataatca
    11461 gtttgtcatt agcgatgagc gcgattacgc catttatgtg atttcactga ccccaaactc
    11521 ggaagtaaaa atcctcaaaa aaataaaaat cccgctgcaa gactccccaa ccaattgcgg
    11581 ttttgaaggg ttggcttatt ctcgccagga tcacactttc tggtttttta aagagaaaaa
    11641 tccgattgag gtctacaaag ttaacgggct gttaagtagc aatgaattac acatcagcaa
    11701 agacgaggca ctacaacggc aatttacttt agatgatgtg tccggcgcag aatttaacca
    11761 gcaaaaaaat acgcttctgg tgctgtctca tgaatctcgc gcactccagg aagtaaccct
    11821 ggttggcgag gttatcggtg aaatatcact aaccaaaggt agtcgaggtt tgtcgcataa
    11881 catcaaacag gccgaaggga ttgcgatgga tgcctctggt aatatttata tcgttagcga
    11941 accaaatcgt ttttatcgct ttacccctaa atcatcgcat taacaggctg gcatatttca
    12001 cctttttgtt attaacttgt taacaacaaa aatgacatac aaggttaaac atgccttcgt
    12061 cctcgcatcc cgtagaacgc ttttctttca gcaccgcgct tttcgggatg ctggttctga
    12121 ccttaggtat gggattaggt cgcttccttt atacgcctat gttgcccgtc atgatggcgg
    12181 aaggggcgtt ttcatttagc cagctctcgt ggattgccag cggcaactat gcggggtatc
    12241 tggctggcag tctgctattt tcgtttggcg catttcacca gccgtcgcgc ctgcgcccat
    12301 ttctgttggt ttctgccctg gcgagcgggt tgttgatcct cgcaatggca tggttgccgc
    12361 catttattct ggtgttattg attcgcttcc tggcgggtgt cgccagcgcc ggtatgctga
    12421 tttttggttc gacgctgatt atgcagcaca cgcgccatcc ttttgtgctg gcagctttgt
    12481 tttctggcgt tggcattggc attgcactgg gcaacgaata tgttctggca ggcctgcatt
    12541 ttgccttatc ctcgcaaacg ttatggcaag gcgccggggc gctttctggc atgatgctga
    12601 ttgcacttac gcttttaatg ccctcgaaaa aacacgccat cgcaccaatg ccattggcaa
    12661 aaacggagca acagataatg agctggtggt tactggctat tctgtacggc ctggcgggat
    12721 ttggttatat catcgtcgcg acttacctgc cgctcatggc gaaagatgca ggctcaccat
    12781 tgttaaccgc ccatctctgg acgttagtcg gcttatcaat tgtgccaggc tgctttggct
    12841 ggctatgggc agcaaaacgt tggggggctt tgccttgcct gacggcgaat ttgctggtgc
    12901 aggctatcag tgtgctgctt actctcgcca gcgactctcc tctcttgctt atcattagta
    12961 gtatcggttt tggcggtacc tttatgggca cgacatctct ggtgatgact atcgcccgcc
    13021 aactgagcgt tccgggaaat ctcaatcttt taggctttgt gacgctcatt tacggcattg
    13081 gacaaatcct cggcccggcg ctgaccagta tgctcggcaa tggaacatcg gcgcttgccg
    13141 gcgccactct ctgcggcgcg gcggcgttgt ttattgctgc gcttatcagc acagtacaat
    13201 tatttaagct gcaagtggtc acttcatgag gagtgtttcc cttaccactc aagaaattcg
    13261 accgcaaccg ttgatgtcag ccagaccccg ttttccgttt gccagaaagg gatgcctcgt
    13321 ttcgccattt cctgtgcttt gacggttaag ataaccggcg atccgtgtct tgctcccact
    13381 ttgcgtgccg ttgcttcatc agcagagaga tgtacgtagt gacgctcacc tgcaatcagc
    13441 ccctgttttt ttatctcgtc aaggaaacgg cttgccgtac cgtggtaaag aaactgtggc
    13501 ggtgtttttt cggcaaagga aatagcgacc tgcgaagtca aatgcccctg aaccgcgcgg
    13561 atgcacctac catcactgga ataactaaaa cgctttttat cactggtggc gaccacggta
    13621 tccagcaatg tacgggttaa tcgcttgccc gctttttggg cacaaagtat gagcttatcg
    13681 atatccgccc atccttcacg gtcaagtaca ataccaatcg cctccggttt gtgtcggagg
    13741 acaaaactta aaaacttgct cgtatccgcc agctcttttt cgttgtactt tgccatctcg
    13801 ttttcgtccc tggattttct gcttttattt actgatgtca ttgtcactgg gtttatgaat
    13861 tttctgcaac tcaatcctgc gcagagcctt ttccacacag cgtaattttt ccgctatgca
    13921 aaaaacgcat agccagaaac atacttgcat tttccccata cccacatggc cgataaggtt
    13981 tttcagctcc aggaattttc ctggctcaag caacacacaa acacaaaaaa acaatgggga
    14041 ttgttatgac acaacaaggg gatgctgtag ccggggatct ggcgacagaa aaagtgggca
    14101 ttaaagggta tctggctttt tttctcacca taatcttttt ttccggtgtt ttttccggga
    14161 cagacagctg gtggcgcgtt tttgattttt ccgttctgaa cggatcgttt gggcaactgc
    14221 ctggtgcgaa cggagccacc acctcatttc gcggggcggg tggcgcgggg gctaaagatg
    14281 gctttctctt tgcgctggag ctggcaccgt cagtcattct ctctctgggg attatttcaa
    14341 ttaccgatgg tcttggtgga ttacgcgccg cgcagcagtt aatgacacct gtgctaaaac
    14401 cgttgttggg cattccaggg atctgctcgc tggcgctgat cgctaacctg caaaacactg
    14461 atgccgccgc cggtatgacc aaagagctgg cgcaggaagg ggaaattacc gaacgcgata
    14521 aagtgatttt cgccgcatat caaaccagcg gtagcgccat catcaccaac tacttctctt
    14581 ccggcgtcgc ggtgttcgcc tttctgggca cgtccgtaat cgtaccttta gccgtcatcc
    14641 tggtgtttaa atttgtcgga gccaacattt tgcgcgtctg gcttaacttc gaagaacgtc
    14701 gcaacccaac gcaaggagcg caagcatgac aactcaggtc cgcaaaaacg tcatggatat
    14761 gtttatcgat ggcgcgcgcc gtggctttac tatcgccacg accaacctgc tgccaaacgt
    14821 ggtgatggcg tttgtcatca ttcaggcgct gaaaattacc ggcctgctcg actgggtggg
    14881 gcatatctgt gaacccgtaa tggcgctgtg gggattaccc ggtgaagcgg caaccgtcct
    14941 tttagccgca ttaatgagta tgggtggcgc agtcggtgtt gccgccagtc tggcaaccgc
    15001 tggcgcatta accggtcacg atgtcaccgt tttactgccc gcgatgtatt taatgggcaa
    15061 cccggtgcaa aatgtcggtc gctgcctggg gacagccgaa gtgaatgcca aatattaccc
    15121 gcatatcatt acggtctgcg tgattaacgc attgctgtcg atctgggtta tgcagcttat
    15181 tgtttaacaa ggagttacca tgattgatta taccgcagcc ggttttaccc tgctgcaggg
    15241 agcgcatttg tatgcgccgg aagatcgggg aatttgcgat gtcctcgtcg ctaacggcaa
    15301 aattatcgcc gttgccagca atatcccttc tgacattgta ccggactgca cggttgtcga
    15361 tctcagcggg cagatcctct ttcctggttt tattgatcaa cacgtccatt tgattggtgg
    15421 tggcggtgaa gcaggtccca cgacgcgcac gccagaagtg gcgctaagtc gcctgacgga
    15481 agcgggcgtc acgtcagtgg ttggtctgct gggcaccgac tctatctctc gccacccgga
    15541 atccctgctc gccaagaccc gtgcgctcaa tgaagaaggc atcagcgcct ggatgctgac
    15601 cggcgcttat catgtccctt cccgcaccat tacaggttcc gtggaaaaag acgtggcgat
    15661 tatcgatcgt gtgattggcg tgaaatgcgc catctctgat caccgttctg ccgcaccgga
    15721 cgtttatcac ctggctaata tggcggcgga atcccgcgtt ggcggtttgc tcggcggtaa
    15781 acctggcgtc accgtgttcc acatgggcga cagcaaaaag gcgttacagc ctgtctatga
    15841 cctgctggaa aactgcgatg tgccgatcag caagttgctg ccgacccacg ttaaccgcaa
    15901 cgttccgttg tttgagcagg cgctggagtt cgcgcgcaaa ggcggcacta tcgatatcac
    15961 cagcagcatt gacgaaccgg tcgcccctgc cgaaggtatt gcccgcgccg ttcaggcggg
    16021 tattccgctg gcacgcgtca ccctcagctc cgacggcaac ggtagccagc cgttcttcga
    16081 tgacgaaggg aatttaaccc atatcggtgt tgccggtttt gaaacgttgc tggaaaccgt
    16141 gcaggtgctg gtcaaagact atgatttcag tatcagcgat gccctgcgcc cgctcaccag
    16201 tagcgtagcc ggtttcctta acctgaccgg gaaaggcgaa attctgccag gcaatgatgc
    16261 tgacttgctg gtcatgacgc cagaactgcg cattgagcag gtatacgctc gcggcaaact
    16321 gatggtcaaa gacggcaaag cctgcgtgaa aggaacgttt gaaacggctt aaacggttgt
    16381 cagccgcccg aatggacgta gaatgcccgc aagggcggct gacagagtaa aacgtaatgg
    16441 atgactgtgg tgcgattttg cataatattg aaaccaaatg gctttatgat tttctgaccc
    16501 tggaaaaatg ccgcaatttt tcccaggcgg cagtcagtcg caacgtctcg caaccggcat
    16561 tcagccgccg catccgtgcg ctggaacagg cgattggtgt tgaattgttt aaccgccagg
    16621 taacgccgct gcaactctca gaacaaggga aaatcttcca ttcgcagatc cgccatctgt
    16681 tgcaacagtt agagagcaat ctggcagagc tgcgtggcgg cagcgattac gcgcaacgta
    16741 aaatcaagat agccgctgca cactctcttt ccctcgggct gttaccgtcc attatcagcc
    16801 agatgccgcc gctctttacc tgggcaattg aagctattga tgtcgatgaa gcggtcgata
    16861 aactgcgtga agggcaaagt gactgtattt tttcctttca cgacgaagat ttgctggaag
    16921 cgccgtttga ccacattcgc ttatttgaat ctcaattgtt ccctgtctgc gccagtgacg
    16981 aacacggaga agcacttttt aacctcgcgc agccacactt tccgttactg aattacagcc
    17041 gcaactccta catggggcga ttgattaatc gcaccctgac gcgccacagt gagttaagtt
    17101 tcagcacctt ttttgtctct tcgatgagcg agcttttaaa gcaggttgcc ctcgacggct
    17161 gtgggattgc ctggctgccg gaatacgcta tccaacaaga aattcgcagc gggcagctcg
    17221 ttgtgctgaa tcgggacgaa ctggtgatcc cgatccagga tctacgcata ccggatgaat
    17281 actcgcatga atcccgttgc cgaacgcttc tggcgtgaac tgcgcgagct ggagattgtg
    17341 cttagctgac attctccagc atcgcactac accaaccgcc attatcccag accagatcaa
    17401 acgttagcgg ttcaccgtcg tggtacagca ttatctcaag gctgatcgcc agagtgtggc
    17461 agtacgcttc tttaaaatta acctgcaacg ccgcaatccg tggctcatga aataacagtg
    17521 cagaacgcag ctgacggcaa taccagctct ggagatgttt gtcgctctgg ttatgcactt
    17581 gccactcggg catgcctgaa acaagtaatg acggtaatga cgaatgagga gaattaaaaa
    17641 gagtgaggag atcgctgcat accgatttac gcagcaatga cgttttattg cctgaaattt
    17701 caggtaaaac agcctgacgt gattggcgca tcattttgcg cactcctgac gtttagcaac
    17761 atatgcggct tgccctgaac accgggcacc actgtattga tgctcatttt ccatagccat
    17821 tcccttttgt tcattacatc catgtcagtc tgagacaggc ctgaatcctg tcagcaaagt
    17881 ataaaacctt acttgcctat gaatatctac tgacttttca ggaaaaaagt catgatttga
    17941 taaaaatcat caaactcaaa aaaaaaccgc tagtatattc tgaatattcc gaatagttac
    18001 gtagatgaaa atacttgtca ccaccgctac ttaattccgg tgtgaaatta tttatttccg
    18061 cataatcagc catgtattat ttgaaacaat tagttt
//
LOCUS       DAZGLJ010000085        18021 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000085 DAZGLJ010000000
VERSION     DAZGLJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18021
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.085"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(50..625)
                     /locus_tag="ACHK49_002515"
     CDS             complement(50..625)
                     /locus_tag="ACHK49_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO9263146.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(694..1272)
                     /gene="terD"
                     /locus_tag="ACHK49_002516"
     CDS             complement(694..1272)
                     /gene="terD"
                     /locus_tag="ACHK49_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFO9263147.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(1321..2361)
                     /gene="terC"
                     /locus_tag="ACHK49_002517"
     CDS             complement(1321..2361)
                     /gene="terC"
                     /locus_tag="ACHK49_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFO9263148.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(2384..2839)
                     /locus_tag="ACHK49_002518"
     CDS             complement(2384..2839)
                     /locus_tag="ACHK49_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO9263149.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(2862..4019)
                     /locus_tag="ACHK49_002519"
     CDS             complement(2862..4019)
                     /locus_tag="ACHK49_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO9263150.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(4019..4600)
                     /locus_tag="ACHK49_002520"
     CDS             complement(4019..4600)
                     /locus_tag="ACHK49_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO9263151.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            4923..5981
                     /locus_tag="ACHK49_002521"
     CDS             4923..5981
                     /locus_tag="ACHK49_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFO9263152.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            5991..7133
                     /locus_tag="ACHK49_002522"
     CDS             5991..7133
                     /locus_tag="ACHK49_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFO9263153.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            7126..7899
                     /locus_tag="ACHK49_002523"
     CDS             7126..7899
                     /locus_tag="ACHK49_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263154.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            7901..8980
                     /locus_tag="ACHK49_002524"
     CDS             7901..8980
                     /locus_tag="ACHK49_002524"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFO9263155.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            8980..9936
                     /locus_tag="ACHK49_002525"
     CDS             8980..9936
                     /locus_tag="ACHK49_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFO9263156.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            9947..11155
                     /locus_tag="ACHK49_002526"
     CDS             9947..11155
                     /locus_tag="ACHK49_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFO9263157.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            11173..11640
                     /gene="terW"
                     /locus_tag="ACHK49_002527"
     CDS             11173..11640
                     /gene="terW"
                     /locus_tag="ACHK49_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFO9263158.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            11901..12230
                     /locus_tag="ACHK49_002528"
     CDS             11901..12230
                     /locus_tag="ACHK49_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9263159.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            12217..12597
                     /gene="tnpB"
                     /locus_tag="ACHK49_002529"
     CDS             12217..12597
                     /gene="tnpB"
                     /locus_tag="ACHK49_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9263160.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQEEPEDAQSNAITQ"
     gene            12640..13470
                     /locus_tag="ACHK49_002530"
                     /pseudo
     CDS             12640..13470
                     /locus_tag="ACHK49_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(13501..15114)
                     /locus_tag="ACHK49_002531"
     CDS             complement(13501..15114)
                     /locus_tag="ACHK49_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFO9263161.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(15145..15495)
                     /gene="tnpB"
                     /locus_tag="ACHK49_002532"
     CDS             complement(15145..15495)
                     /gene="tnpB"
                     /locus_tag="ACHK49_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9263162.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(15492..15917)
                     /locus_tag="ACHK49_002533"
     CDS             complement(15492..15917)
                     /locus_tag="ACHK49_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9263163.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            15971..16722
                     /locus_tag="ACHK49_002534"
                     /pseudo
     CDS             15971..16722
                     /locus_tag="ACHK49_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         4388 a         4764 c         4617 g         4252 t
ORIGIN      
        1 cgatacccaa aaaaaacccc ggcattgccg gggtcagact cagcgtcttt tagacattga
       61 cgccgtgctg ggaagcaagc gctgacagac caccggcaaa accctggcca acagctttaa
      121 acttccactc agtaccatgg cgatacagtt caccgaagac cattgcggtt tcggttgagg
      181 cgtcttcaga cagatcgaaa cgggcaattt ccgtcccgtt gtcgttgttg taaacgcgca
      241 tgaagctgtt gctcaccatg ccgaagtttt gtttacgcgc ttctgcatca tagatggtaa
      301 cggcaaatac cagttttttg atgtctgctg agactttggt cagatcgatt ttgacctgct
      361 catcgtcgcc gtcgccttca ccggtacggt tgtcgccctg gtgctctact gcgccatcag
      421 ggctggtttt attattgaag aaaatgaaat gggcatctga cagtacttta ccgtcttcac
      481 ctactgcgaa tacggaagcg tccagatcaa aaccctgacc atcggttaca cgggcatccc
      541 agcccaggcc aaccatagcg acattcatgg ttggtgcttc tttggtcaga gatacgttgc
      601 cgccttttac gagagaaact gccattttta gctcctgcaa acagttgaat gaggctgaat
      661 taacagcccc agatatgaaa agttactttt cgatcaggac gcgttaatgc cgtactgagc
      721 acatacagat gccagaccac cagcataacc ctggcctact gcgcggaatt tccactcacc
      781 attgtggcga tacagctcgc cgaacagcat ggcagtctca gtggacgcat cttcggtcag
      841 atcgtagcga gcgacttcag tctggttatc gtcattaacc agacgaataa acgcaccgga
      901 tacctgacca aagctctggc gacgagcctg agcatcgtgg atggtcacaa cgaagatgat
      961 cttgtcaact tcagacggga cggcgtccag tttaattttc agcgattcat catcaccatc
     1021 gccctcaccg gtgcggttat cgccggtgtg cgttacggaa ccgtcggatg acgtcaggtt
     1081 gttatagaag atgaaatctg aatcgccgcg cactttgccg tttgaggcca gcaggaatgc
     1141 tgaagcatcc aggtcaaagt cctgaccgtc tgttgaacgc gcatcccagc caaggcccac
     1201 caggacattt ttcattgacg gagctgcttt actcagggag acgttcccgc ctttggaaag
     1261 agaaacactc ataaaataac ctcttcgatt agtaattgtt caggttaaca cttaagggga
     1321 ttaactcccc ttttcctcag attcaggttt gcccgggaac atgacgcttg cgagaatgcc
     1381 cagcgccagt acacccagta caacatacag gctggttgtt gccgcgatgc tgtaaccatg
     1441 atgccagatg tgatcggtcg cattcaggcc gagttttgcc gcgatgaaga acagcagcac
     1501 gataacggcc ttctccagat gaaccaggta ctgtttcagt gcctcaagga caaaatacag
     1561 agtacgcagg cccaggatag caaacatcat ggcactatag acgataagcg gttcacgact
     1621 gacggcaatg attgccggta ccgagtcaaa cgcgaacatt acgtccgaga gttcaaccac
     1681 agccacacac aggaacagcg gggtcgcata cagcgccgct tttttaccac ggccaatggt
     1741 gacatcgctg ttttctggtt tcgccagttc agcatccact tccttctggt taagtaggaa
     1801 ggcatgccct ctgagcttcg gccagatagg gaagaagcgt ttaaccatac ggtaagccag
     1861 atgctgggag taatcctcaa tttcatcatc gtcatcaccg cttttaagca tcatgaccgc
     1921 cgtccaggca acgataatag cgaagacaac ttcaacgtac ggccccagac tcagcaggct
     1981 cgtaccgatg gcgacaaaga tgcccctgaa gacgatggca ccaatgatcc cccagtagag
     2041 aacacggtgg cgataacgat ccggaacggc gaaccaggag aagattgcca tcatgacgaa
     2101 caggttatcg accgacagca ctttctccag cgcataaccc gtgacaaaca gactggcaac
     2161 ctcagcaccg tggtggatat agaggaaacc ggcaaatgcc atcgcaacca caacccagaa
     2221 tacggaccag agcgcagcac tcttcagcga gattggcttg tcatcacggt gcataaacag
     2281 gtcaataaag atggcaccga ctgaaagcgc aataaaaaca atgaccgttt cagtcgggaa
     2341 gccgatgtgt gtggaaacca tattttaccc ttagggatgt ggatcagagg ccaaattcag
     2401 ccgggtcgaa gcccaacgcg atagcgatct cgcgggaggc cagtttctcg tcctgatcga
     2461 agttaccgtc acttttcgca acggcaatac caacacgtaa ggccagctga gcggcctcag
     2521 gctgatcttt cagcgccagg atgtatttca tggtttcgcc cttgccaatt tcaacatcga
     2581 aatcgaagct tgaaaccagt ttattgaaga actcaatcac ctcattggta tcgaagacct
     2641 tcagctcttc tgaagagcgc agaaagccca tcattttctg cttttcttcc gaacttacgc
     2701 cgtcactcga tacggcaata cgggcacata cagcaacggt gccctgcatg aattttttgt
     2761 ttttgaaacg gccaacctgg cgggtcagtt cgtcacggcc tgagttaatg gcacctttaa
     2821 ctttgtcgaa aaagctcata cctgtttcct ttgttgctgt gttatttaga accggcgctc
     2881 cagcgaaatc cccagccaaa tgcccggtcc atttcgtcct ggcctttgaa gtactggtta
     2941 attcgctcga ctttaattgc gccgttttcg tttacgagtc tggcaatggc gcacaatgtg
     3001 cgacggttct caccttcggt caggcgggtc tcaatgggcg gttgatccgg cacatgaatg
     3061 gtgactacac catcagtctt gtcccagctg ggaacacctt cataaataaa ggcgtaaatc
     3121 agcacttcgc ggatatgctt ccattcacgt ccattgatat gcagccactc gccgtctgat
     3181 acatcacccg tccggtcatc gcccttgagc tgaacatacg gttcatcgcg ataatcaccg
     3241 aatgcattac cgagcgcctg aatgaccgat ttatacccgt cctgaagctc aacaaaggcc
     3301 cccagatcca ggtcgattcc tttggagccg aacatacctg caaagcctga tttgctactg
     3361 ccccggtgcc agttcaggtt gatccggatt tcaccgaagt tatcccgttt ggtcaggctg
     3421 attgcaggct tctcttttgt cagcgatacc ttgctcaggc tgatttttgt ctcaactggc
     3481 ggaggggtta caaccggagg agttggggct gcgggagccg gttcgtcggc aatatttacg
     3541 ccgaaatgct ctgccagcgg tttaagtcca ccgttgaacc cctgcgcaac gaagcggaat
     3601 ttccagtcat tattacgacg gtaaaattcc cccagaatta aggctgcttc ctgacggccg
     3661 ctcagttcga cactgccgct aaccaggccg gtagcccctt gctccacgtc gatggaaaga
     3721 ttacgaagac cggacaccgt ctgcccaccg tcacaggtca cggtgaacgc aattttctgg
     3781 acatcaggtt tcagccggtt gagcgcgaca gtaaaggttg agtactggcc ttcactgacc
     3841 aggctgacgg taccatcatc gttacgtggc tgaccataaa acaccatgtc cgcatcaccc
     3901 tgcaccttcc cgtcggcata caggcgaaac gccgaagcat ccacctggcc gcccgaggtg
     3961 atccggaccc gcaactcctg ggctggaacg ggggcattgc cccccggcgt caggttcatt
     4021 agcgaataac cgctgaaacg atatcggagt gcatatcgtc aattgtgcgg ccgcgacagg
     4081 catggccaag ggcggtaaag tcccagttgc cgttattacg gcgcagcgaa gaaataacga
     4141 tcccggtatg tgagccctgc tcagtcagct tgtagcgagc cagctctttg ccggtttgat
     4201 cgacaacgcg gcagaaagcg ttttcgacgt cattgaacga ctgaccacgg aagctgttta
     4261 ccgtaaacgc caggtattca acgtttgcag gcaggcggga gaggttgacc ttaatcactt
     4321 catcgtcacc gtcaccctca ccggtcaggt tatcgccact gtgcacaaca gcgccgcagg
     4381 tggatttcag tttgcggaac cagatggtgt caattgtttt acctgtgctg tccattaata
     4441 cacagccagc atcgagatcg attgagtcgt tgcctccaaa caatcctcca agaagacctt
     4501 ttttcttaac cggatcccaa ccgagaccga aatgaagctg gcttaatgca gatgattctt
     4561 tgctgagtga taccgtctgg ttcttaacta atgaaaccat gttttattcc ttaaaatggt
     4621 aatgaccgag acaatcaaag gtatatttag taactgacga agaaaatcat atcatgatga
     4681 agttctctga caagggaaat ttttgtggtt ttctgtaaaa acacaaaaag aaatcctttt
     4741 cagggtgtta gtttttctat ttttaattta aatatcaatg tgttataagg aatccaaaaa
     4801 acactgacgc actccataac cacaggagtt attaatcata ttatgattga catgtagctt
     4861 agacactcat agactcactt ctcaatcata aatttgcatg gcgttccgga taattaaaaa
     4921 aaatgaacaa gaaaatttgg tttatggaag gtttatcctc ccagcgagat attattcagg
     4981 gggtaaaatc atttgcacaa aaaaataatt ttgccattac cgtttttgcc tcccaccgta
     5041 acgaaagaaa tgaaatcctt tccgttgccg attattcttt gactgaacct gaagatcctc
     5101 aaaaacgtct tcagtttatc caggaaacca ttcagaccta cggcatccac catattcata
     5161 ctggccgtaa cagccagtgg tttgaagaac accgttcagc cattgaaccg accggtgcca
     5221 cccttactac cggtgcaacg ggcgtcgact ggttaactct ggctgacgaa aaagttactt
     5281 ttgctcagtt tatggagcaa aagggtctcc cggtcgtacc atcctggcgg gtgaatacgc
     5341 tggcagaatt aaagacacac ctcgcggccc cgccgttcac tgacagcccg gtatgcgtga
     5401 agccggtgac gggtatctat ggcatgggat tctggcgctt tgatgacagt gcttcgccta
     5461 tggccgtctt taatcatccc gaacatcgtc tggtcagtcc gcaacagtat attgcagcag
     5521 catcagctgc tgagtcgttt aaaccccttg ttttgatgcc gtacctgcca ggcccggaat
     5581 tttccgtcga tatcctcgcg gataagggcg aaatactcgc agccgtggga cgccgtaagg
     5641 aaggggctat ccagtatctg gtaaacgaag gaagcgcctg ggaactggcg tgtgactgcg
     5701 cccgtgttat gaaggccgac gggctggtga atgttcagac gcgaaacgat gtgaatggca
     5761 acccggtgct gcttgaaacc aacatgcgtc cgtcaggggg ggtgggttat acccttcaca
     5821 gcggtgtgaa ccttcctggg ttatttgctg cctttaagct cggtctgatg tctgaagata
     5881 tggtacgcca gagcgctaaa aacacctttt ctccggttgc ggtgagatcc attacggatg
     5941 taattgcata cccggaatca ctctctaacc ttctgaatta aggcgtattt atgtctgata
     6001 tcctcgaaga tatgatttat cgccgtaccc tctcatgcgg tacgattcag gtaacccgtg
     6061 accagggtga agtttcgctc gatgacctgt ttgacatcgc tgaaagacgt aacccgaaac
     6121 gcgcctttct gtttgtcagc aaagtactcg gcaggcacat tcccgtgcca ccctcagtca
     6181 tgcgacaggc ctacagacag cttgccagcc agttcccctc gacactgaca ggacccgtac
     6241 tgtttatcgg tatggctgaa accgccgttg ggcttggggc cggtgtattt gatgaagtgc
     6301 gccaccagca tcgtgaatct gtctatctga cctctacccg tcacccggtt gatggcacgt
     6361 tgctttgcga gttcaaggaa gaacacagcc acgccaccga tcatctgatc tatctgccag
     6421 atgatgaaga gaaaagacgt cgtgttacca acgcacgaac gctggttttg attgatgacg
     6481 aggcaaccac cggtaatacc tttattaacc tgctttctgc cctgcgtaat accggcaagc
     6541 ttcaacatat tgaacaggtt atcgccgtaa cgctgaccga ctggagtggc aatgccctgt
     6601 cggagcgcac ccccttgccc gtcacttcgg tttcgcttgt aagcggtcag tggggatgga
     6661 ccccattacc tgatgcacct gtcccggaca tgccgaaagt caacgtaact tcacgaggag
     6721 aatgggacat ccaggggaaa cagtcatggg gccgactggg gatgctcgca cctgcggccg
     6781 atctcggcca tgaggtctcc gtccacaagg gggaacgtat tctggttctc gggaccgggg
     6841 aattcgtctg ggagccgttc ctgcttgctg aacggctcga agctgccgga gcacaggcat
     6901 tttatggatc gaccacccgc tcccctgtcg ccgttggtta tgccattgag tccgccattt
     6961 cctttacgga taactacggg ctgggcatcc ccaattttgt ctataacgtc gcccaccagc
     7021 agtttgaccg cattcttgtg tgtactgaga cacccgcaga aagtattgac acgcagcttc
     7081 ttaaagcgct ggctgaggtt gcgcccgtcg tggagattgt tacctatgaa taaacccgtt
     7141 gttctgagcg accttgacga cacgctcttt cagacccgtc gtaaaatggt ggacgagctg
     7201 gccctggagc cattccgtac cggtgccgtt gatcgcaccc tgaatccacg aagctttatg
     7261 acggaagaac agtccatgtt ggtggactgg ctcctggagc aggctgaact catacccgtt
     7321 accgcacggg gaactgaaga aatcagccgc gtacggatcc ctttccactc ctgggcaatc
     7381 acaacgcacg gggccgtcat tctcacgcct gaaggcaaac ccgacgagga gtggaaggcc
     7441 catatgctcg gccagctggc tccctaccag gaaaagctga catcgatgca gcgtctgatc
     7501 actgaaatga tggacgcaaa aggaatcaat gcctgggcaa ggctgaactt cgaatacggt
     7561 gaaacggcgg tttatatggt gatgaaacac cgcgacagca cccgccttga cgagctcaat
     7621 gccattgcag atgagataga aacggtgttt ccgaccgagg gcttctacat ccaccgcaac
     7681 agtaataacg tggcctggct tcccaccccg gttgagaaag ggctggcagt cagctggctg
     7741 cttgaaaaac ttcgggctga acgaggagtt ttccccgtaa ttggtttggg cgacagcctg
     7801 agcgatcatc gttttatgaa actgtgcagc tggtttggca tcccgcgtca gagtcagttt
     7861 gcagatgcca tttcacagcg aatttttgga gaaaattaaa atgcagccac atgaaacatt
     7921 taccggctca taccagcccg gtgacgtgga atttctgcta aagccggttg tcattgagat
     7981 gacgccggtt gagaaaaaag aagaactgat tcagtccggg aagaaacatt attcggacat
     8041 gctcagccag gagccagcgc ctacacaatg gcatcttgat ttgtttcacc gggcgctgga
     8101 tcggggcgca gagaggcttg caaaagaagt cacacagctc gctattgctc tggccgaacg
     8161 cttcggtgat gagcccattg tactggccag tcttgtcagg gctggcgttc cgctcggcgt
     8221 tatgctgcac caggccctgc gtgacatggg gaaaacctca tggcattacg gtatcagcat
     8281 tatccgggat cgtggaattg acggtgcagc cctcgacgtc attgaagagc ggcatggtac
     8341 cagcggtatt gtctttgttg acggatggac aggtaaaggc gcaattaccg gagagcttgt
     8401 acgcgcactg aaagatcgcc cgggctatcc tgagcagccg cgactggttg tcctggccga
     8461 tccctgtggc tgctcctggc ttgcagccag tgatgatgac tggctcattc cttttggcat
     8521 catgggggcg ccggtatcag gcctgatctc ccggtcagta tggtcctctg aaggattaca
     8581 cgggtgcatg gtttgcgagc atctcagtga attcgaatgc agccggatgc ttgccgatac
     8641 cgtcgctcat ttccgtaaga agttaacacc gtcatccctc gctcccctga gctggaatac
     8701 ggaatcagcc cgggtcttat ggcagacaag tcgcgacgtt atcgcgttcc tggccgatga
     8761 atttaaagtg gacagcgtca atcgtattaa acccggtatt gctgaagcaa cccgggctgt
     8821 attacgtcgg gtaccggacc atgtatttgt gcgttctatt gacgacccgg acgttgcctt
     8881 gcttgtaggg cttgctcgtg aaaagggaat agttgttaca gaaatggggg gaaccctcgg
     8941 ccagtatcgg gctgtaacca ttatcaagaa ggtactctga tgaaaaatcg tctttctccc
     9001 tggaatctgg gagccacgct atacatgcct gcaacacggg aagatattgc tgatgccgtc
     9061 ctgcacggga aaatcccggg cttgcgctct ctggtgattt gcctggagga tgctgtcagt
     9121 gaagcagaca tccccgtcgc cctcaaaaat ctggagcacc tgctgcacga gctcagtaac
     9181 agcatgagta gcctgggtaa aaatgactgg cctctggtgt tcattcgccc ccggcatgcc
     9241 gaaatgggca gatggttaaa agcgcattat gatctttccg ctgtcgacgg gtttgtgctg
     9301 cctaaattta ccctcagttc gttagctgaa tggtgggata tcatggtcgg gactcacctg
     9361 tgcatgatgc caacgctgga aacagaagat gtctttgacg tggttcagat gcgcgagctg
     9421 gctacccgtc tggaggaaca tccctgccac gaccgcatta ttgcgcttcg aatcggcggc
     9481 aacgatctta tgaacgttgt gtcgcttcgc cgtccccggg acctgacgct gtatgacagc
     9541 ccgatgggct acgtcatcaa aatgctcgtt tcggtcttcg gcccgcgtga ttttgccctg
     9601 accgcgcctg tatgtgagca tattgacgat catgccgtta tggccagaga gctggctctt
     9661 gatatggcac acgggcttgt tgggaaaacg gccattcacc cgggtcagat agaggtcatt
     9721 caaaacgcgc tgatggtcac tcagggtgag cattctgacg ctctgcggat cctgaactct
     9781 acccaggccg tgtttaagtc gcagggagca atgtgtgaac ccgccacaca tcgccgctgg
     9841 gcggctggca ttctggacag ggctcgtttt tatgggttac agaacgagca aagcgctgat
     9901 ggaatcagat tacttaccgt gacccagcat cattaaggaa atcataatgg ggttcaggtt
     9961 cagaaagtcg atcaacatta ttcctggcgt tcgcctcaac ctgagtaacg gtgcaccgag
    10021 cctgagtgtc gggccgagag gtgcttccgt ttcttttggt agccggggga cctatgccaa
    10081 tctgggcttg cccggtaccg ggctgagtta ccgtacccgg cttgaccggg ccgcgcgttc
    10141 cagaggtgaa aaccggacgg caaccgaccc ggggctcaga caggcgcttg agcagaaagc
    10201 cgctgaactc atgtcagcgg taaccgcaat ccgtaatatt cacgagctga cgccggatcc
    10261 aaaaacaggc atcagctggg cagagctgga agcagtatac ctgcataaca gaacgtcgcc
    10321 ttttcaggtt ccggcaccgg tgcgtccaga aaagccagac taccttgtat tgccggaaaa
    10381 gcctgccgag agcgaaggca ttagtttttt gggtaaatgg tttgaatcgg aatcagctaa
    10441 agctgagcgc cacgccgaaa atcttcgccg gtggcagcag gagctgattg atgtggagcg
    10501 tgagaatacc cttcgacagc accggtacca gcaacaacgg acggcctggg ccgaacagta
    10561 tgcaaactgg aagtttgaag ctgaagaaca tgaaaaacgg ctcgccacgg ctcaggcaga
    10621 tgcccggcag cagttccgga cagacgccgc gtttttcgaa tcatacctgg cgggtgtgct
    10681 ggcagaaact gaatggccgc gtgaaacgct tgttgcattt gaagtaaagc cggagctatc
    10741 agcagtcctg ctggacgttg atttagctga gattgaagat ttccctgata agatttacgg
    10801 cgttaatgcc cggggaacgg agctgacgga aaaagccatg acgcaaaaag ccgtacgcga
    10861 aaactatgcc caccacgtcc atggctgctt gttccgcctg gtcggtatcg ttttacatac
    10921 gctacctttc gacaacgtga ttgtgtcagg ctttacgcaa cgggtcagta agcggaccgg
    10981 ctatctggag gatgagtata tcctgtcctg caaatgcact cgcagccaga tgtcgtcagt
    11041 aaattttgca ggcataaaac acattgatcc ggttgaagcg ttaggcgatc aaccggttat
    11101 tcgaaagatg agcagtacct tcatttttca gcctattgaa ccactaaccc tttaagcagg
    11161 ctaaatctta cgatgcaatt aaataccaga caggcccgga tttttaagct tgccaactta
    11221 ctgggcaccg gcaagcccgt ttccgctgcc gacattataa ccagtctgga atgctctgag
    11281 ccgacattaa cccgggccct caaagagcta cgcgagtcat attctgcgga gatcaaatac
    11341 agcaaggcag gtcattccta tcatctcgtt aatccgggcc agttggataa aaagaccctc
    11401 cgccggatga atgaagcgct cgcacagaat gctgaactga aaaccggtga gtccacaggg
    11461 aaggtcgtac tcgataagga taaaaaaaca gccgtgtcat tatcgctacg catgcggatt
    11521 ctaagaaaaa ttgaccgtct ggctgcgctg agtgactcga cccgaagtga agcggtagag
    11581 aagctggctc tgcactccgt tgatgaactg ataaaagaat acagcgctaa aaagtcctga
    11641 cgctaaatct cagaccgggg tgaaaactcc ggttttgtca tatttcacct ctgtattggc
    11701 gtgatgcaaa atggcatctg aatcgatcat ggtttggcat ccatttccga tcacagtttg
    11761 gcatcacaat cgatcacgat ttggcatgct tccgctcatt gattggcatc ctggtgtcgt
    11821 tacggggaag tcatcttcac agggatcttc agtcagttag cattaccgac atccattcac
    11881 caacttaccg gagataatca atgtcaaaac cccgatggac gcttgatcag aaaaaacatc
    11941 acgttgccgc atggcgcgcc agcggcctta cccgtgaaca gtactgtgaa ctttacgaca
    12001 ttccgtttaa atccctgcgc cagtggccgc aggatgtcgc aaaagccgaa aaacgagcca
    12061 gagcaccaga aattattcct gtttcagtat ctggctcttc cggaatgact gatggcagac
    12121 cattgtcgga tgagcctgtc actctcttcc tgcccggtgg tatccggatg tgctgccagc
    12181 cttctcagtt aaccgatgtt ttccgggctc ttcgccatgc tgacgcctga taacgtattc
    12241 ctggtcgtaa aacccgtcga tatgcgccgg ggtatcgaca cactgacaca gtacgtccag
    12301 aacgaactta acgctgcatg gcatgacggt gctgcttttg tcttcaccaa taaagtccgc
    12361 tcccgcataa aagttctccg gtgggacaaa catggcgtat ggctgtgtac ccgacgactg
    12421 caccggggaa gcttccgctg gccccggaaa ggtgatgcca catggcatct cacacaggat
    12481 gagtttcact ggctggtctt tggggttgac tggcaacagg tgaaagggca tgacctggca
    12541 aaatgggtat accaggaaga acccgaagat gctcagagca acgcaataac tcaatgaaat
    12601 ataatgaata tccatgcgtg atgtcatata atccaccgca tggatatctc gcttctcagt
    12661 accaccagtg accctgaaca gcttcgcgct ctggcaattg ccatggtgca aaaagtcatg
    12721 gctgagaatg cagaacttca gaaccgtatc cgcatcctgg aagagcagat gaagctcgca
    12781 cgccagcagc gtttcggtaa aaaatgcgaa tcgctggccg ggatgcagcg ttccctgttc
    12841 gaagaggatg ttgacgcgga tatcgcagaa atctcggcgc atctggacaa actgcttcct
    12901 cagaccgggg acgaagaaaa aacaacaaca cgtcctgttc gtaagccctt gccttcacct
    12961 cttccccgtg ctgaaaaggt tatcccgcca gctgaggaac gttgtcctga ctgtgatgcc
    13021 cccctgcact tcattcgcga tgaagtcagc gagaagctgg aatatattcc ggcacaggtc
    13081 gtggtcaacc gctatatccg cccgcagtac agctgtccct gctgtgagaa agtgttcagc
    13141 ggaaaaatgc cggcacatat ccttccgaaa agtgctgttg agccatcggt catcgcacag
    13201 gttgtcatca gcaaatatac cgaccatctg cccctgtatc gccagcagca cattttctcc
    13261 cgaatggggg tggagctccc cgtcagtaca atggcggata tggtcggtgt tgctggcgct
    13321 gcactggccc cgctggcaaa attgttacgc cacgagcttc tgacacggga tgttattcat
    13381 gcagatgaaa cgtccctgag gctccttgat acccggaaag gcggtaaatc ctgctccggc
    13441 tggctgtggg cttacgtcag cggagaaaga gtaagcgtca acggagcacc gtattgacgc
    13501 ttatttattg gtgagtacta cgttccatgg caggagttca tcaactcggt tggaaggcca
    13561 ttctggcagt acgctcagga tatggcgcag atacgcttcc ggatcgatac cgttcagacg
    13621 gcaggtgccg atcagcccgt acaacagtgc tccacgctcg ccgccgtgat cgctgccaaa
    13681 gaacataaag tttttctttc cgagacagac tgcacgaagc gctctttccg cagcattatt
    13741 atccgcctcc gccagaccgt catcactgta ataacagagg gcatcccact gattcagtac
    13801 atagctgcac gcttcgccca gtctggattt tttcgacagc gtaccattct tctccaccat
    13861 ccattcatgc agtgacgtca gtaacacttt gcttcgctgc tgcctgacgg caagacgctc
    13921 tgactccggt aatccccgta tttcatcctc gatggcgtac agttcactga ttcgcttcag
    13981 ggcttcttct gccgtcgcac ttttgctgct gatgtataca tcgtggattt ttcgccgggc
    14041 atgggcccag cacgcaactt ctgtcagtgc accaccttca cgttctgcac tgaacagccg
    14101 atcgtaaccg ctgaacgcat ccgcctgcag gataccccgg aagggacgaa ggtgttgtac
    14161 cggatgtttt ccctgcctgt ctggtgagta ggcgaaccag accgccggtg gctctgatga
    14221 gcccgcattc cggtcatccc ggacatacgt ccagatgcgt cctgtttttg ccttttttct
    14281 gcccggtgcc agcactttta ctggtgtgtc gtcagtgtga accttgcggg tgttcatcac
    14341 gtaacggtac agggtatcat tcagcagagt cattaactga caacatgcgt caacccagtt
    14401 ggagagtaat gcccggctca gttcgatgcc ctggcgggca aagatttcac tctgacgata
    14461 cagtggcagg tgttcgcagt attttcccgt taacacgcgg gcaagtaatc cggagcccgc
    14521 gatgccgcgc tctatcgggc gggacggcgc tggcgcttca actatacagt cacattttgt
    14581 acaggctttt tttacccgaa cagtgcggat cactttcagg gcgctactca ccagttccag
    14641 ctgctcagca ctgacttccc ccagataatc cagctcactg ccacactccg ggcaacaact
    14701 ttcttcaggc tccagacggt gtatttcacg gggaagatgt gccggtaacg gacgacgatg
    14761 acgtgactgt cgcaactggc ggggaacctg cgggtcatcc tcacgcccac tgtaacgatc
    14821 gctttcctgt tcgcgttgtt tcagttgggc ctcagcctgt tcaacctcac gctgcagttt
    14881 ttcagaacgg gtaccgaaca acatccggcg cagtttttct atctgggccc tcagatgttc
    14941 tatttcccgc tcctcctctt cgatcttttc ctcggcacgt gccagtgcag agcgcaggaa
    15001 ggcctccgtc tcttcaacca gactcagttg ctgatctttc tgacggaggg cttcagcctg
    15061 ctcagagagc agcctttcca gctcagtgat acgaatgagg tatttccgac tcatgaccgt
    15121 ttttataatc cggccatgac atttttacaa cattgtcagt gcattaaggc gggatgtttt
    15181 gggctgacgc cagtccagtt tatcgaggag cattgccagc tgcgagcggg taatggatac
    15241 cttaccgtca cgcaccgcag gccagataaa ctggccttcc tccagacgtt tggtgaacag
    15301 gcacagacca tcagcatcag cccacagaat tttaatcgtg tcaccccgtc ggccgcgaaa
    15361 gataaacagg tgaccggaga aggggttctc atccagcaca tgttgtacct gttctcccag
    15421 accgttgaag gatttacgca tatcagtaac gccggcaacc agccagattc gagtgcctga
    15481 tgggagcgag atcatcgtcc cctcccggtc agttcacgga tcaacaccgt gagcagctct
    15541 ggtgaaggat tttccagcgt catgttaccg tggcggaact caactttaca ggaactggca
    15601 ctgactgtgc tttgtgaagg agtggataaa agcggagtaa gagccgccat aggctctttc
    15661 tgctcatcag gcgttatctc aacaggtaat aattcaacgc cagcgccaga agaggttgtt
    15721 accggaagac gccgcgatat acgcctttcg ttctgccaga gcctgagcca tttgaacagg
    15781 aggttatcat tgatatcgtg ttcccgggca atacgggcaa cagaggctcc tggttgtgaa
    15841 gccagtttaa ccatttgaag tttaaactca tttgaaaatg atctgcgggg ttctgaggat
    15901 aatattttct gttccataac aggtgtccac tagttgaaaa ggtgggcacc tacgttacca
    15961 atactggctt aatggctaca tacggcggtc agtttacgct tacgagaaag aagcggtccg
    16021 ccggtcgtct gttttgactc gcagaccgga cgggccctca gatatcctga ggcctggctg
    16081 cagggctggt gtggcggtac gctggtgagt gacggataca gcgtgtacaa atctctggcg
    16141 gataatgacc ccggtattac atctgcctgt tgctggagcc acgcaaggcg cggtttcgca
    16201 aatctgtata aggccagcag ggagccccgt gcagccatgg cgctgagaaa aatcgcgggt
    16261 ctttatcgca ttgagaagtt catccgtgaa cgtcctgtgg aaaaaatacg gcaatggcga
    16321 caacggtatt cccggccgat agtgaatgac ctgtttgcct ggcctgaaga gcaggagccg
    16381 tgctgtccgc ccgacggacc actaaacaaa gcgattaatt atatcctgaa ccggcgcgat
    16441 gaactgagct gcttcctgag tgacggagcc gtgcccctgg acaacaatat ctgcgagcgt
    16501 gctatccgcc cggtggtcat ggggcggaaa gcatggttat tcgccggttc actgatggcc
    16561 gggaaccgcg cggcacagat aatgagcctt ctggaaacgg caaaacgtaa tggtctggag
    16621 ccgcatgcct ggctgacaga cgttctgacg cgtctaccgg agtggccgga agaccgactg
    16681 gaagagttgc tgccccttga gggatttacc ttctccgggt gagtgatacc tgccatcagg
    16741 tgttcgtgca gcgggccata acccgcagtc gggagttgaa cgcctgacga caggaaatga
    16801 gccagagcag cgaccacgcg ggctgacagt gagtcagaac ctgaatcagc ccggagccat
    16861 tcccggggct gtacttcatc acctgccatc agtacccgtg tcagtacttc gcgacctgac
    16921 gcaattaccc gactcagttc ttcagaaccc gcctccgata ccagcctggc ggtcgccccg
    16981 gcgcaacaga ataaatcaac catcgtcaga ctgcgatcat tcgtatgcat cgctgatgat
    17041 gcgcccgaaa aacgcgtcaa tatgagttcg ccggaatatt actgacgaca cggtttgtag
    17101 ttacgttcct tcggtgagtg agtacctgtg cgtcagggta acgcaatcag ttcaggttta
    17161 gcggggtgtc aagtcggttt ggctaaccga ccgcagaatt aactgtacct ttaaaggtcg
    17221 cccccgatat atctgcctgt cagacatatc gccgattcgg tatctcacta cataaaactc
    17281 cttaaaaaac aatgcgattc tgttggtacg aagctttcag taagtcagtc cggagaagcg
    17341 ccagtacgat acagaatgag gttgaaaaaa acgttcacgt ctggtcactt cttcaaatgt
    17401 taaaaaacat ctcgtaaaga gccagtttat gcgggttcgt tgagtagacg tgcgagcgtc
    17461 actttgtcag ctttaacgct gacgaaagtg acgcccgcac agcttcaaac tccacatgca
    17521 cacgcaagcg accaaatttc aaatggtctt atgccgtgcg cacagtcata agaagcgcat
    17581 gcggaaagtc agaaaatatc ttaagacgca gattttaaag gcgtttttat gccagtctgc
    17641 cgggaataca cagtagtccg gttgtccgga agcaccacgg taatccgaag gaaaaatcaa
    17701 cgtacctcaa tcaccgtagt gatatccgtt cccagaggct aaacggatgt accaccataa
    17761 aggcatcctc tttcaggcta cgaggatatt gcaatcaccc gaaggcgttt ctctcagatt
    17821 gctgaaacat tggctggcag tcacccgaag gcgtccctct cagactgctg gaacattggc
    17881 tggtcgcaaa agcgacagtt aatgcaaata caggttaaca aaaagggaaa tctgccgtca
    17941 acccaaccat atgaacatgt tgatttcatt atgaatgtga tgttctaata taaattaatc
    18001 agtaacttca taaaaagaat a
//
LOCUS       DAZGLJ010000086        18012 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000086 DAZGLJ010000000
VERSION     DAZGLJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.086"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..2824)
                     /gene="xdhD"
                     /locus_tag="ACHK49_002535"
     CDS             complement(<1..2824)
                     /gene="xdhD"
                     /locus_tag="ACHK49_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO9263164.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNSDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYNAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLRE"
     gene            complement(2821..3600)
                     /gene="ygfM"
                     /locus_tag="ACHK49_002536"
     CDS             complement(2821..3600)
                     /gene="ygfM"
                     /locus_tag="ACHK49_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO9263165.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(3651..4979)
                     /gene="ssnA"
                     /locus_tag="ACHK49_002537"
     CDS             complement(3651..4979)
                     /gene="ssnA"
                     /locus_tag="ACHK49_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO9263166.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(4982..8080)
                     /gene="ygfK"
                     /locus_tag="ACHK49_002538"
     CDS             complement(4982..8080)
                     /gene="ygfK"
                     /locus_tag="ACHK49_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO9263167.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(8402..8980)
                     /gene="mocA"
                     /locus_tag="ACHK49_002539"
     CDS             complement(8402..8980)
                     /gene="mocA"
                     /locus_tag="ACHK49_002539"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO9263168.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            9083..9853
                     /gene="yqeC"
                     /locus_tag="ACHK49_002540"
     CDS             9083..9853
                     /gene="yqeC"
                     /locus_tag="ACHK49_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO9263169.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            9901..11526
                     /gene="yqeB"
                     /locus_tag="ACHK49_002541"
     CDS             9901..11526
                     /gene="yqeB"
                     /locus_tag="ACHK49_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO9263170.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSTA
                     EAMKLTERGFIPVMADPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(11567..12499)
                     /gene="arcC"
                     /locus_tag="ACHK49_002542"
     CDS             complement(11567..12499)
                     /gene="arcC"
                     /locus_tag="ACHK49_002542"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO9263171.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALLDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(12547..13932)
                     /gene="hyuA"
                     /locus_tag="ACHK49_002543"
     CDS             complement(12547..13932)
                     /gene="hyuA"
                     /locus_tag="ACHK49_002543"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO9263172.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(13985..15196)
                     /gene="ygeY"
                     /locus_tag="ACHK49_002544"
     CDS             complement(13985..15196)
                     /gene="ygeY"
                     /locus_tag="ACHK49_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO9263173.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(15254..16450)
                     /gene="dpaL"
                     /locus_tag="ACHK49_002545"
     CDS             complement(15254..16450)
                     /gene="dpaL"
                     /locus_tag="ACHK49_002545"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO9263174.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(16508..17695)
                     /gene="ygeW"
                     /locus_tag="ACHK49_002546"
     CDS             complement(16508..17695)
                     /gene="ygeW"
                     /locus_tag="ACHK49_002546"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO9263175.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT         4182 a         4525 c         4715 g         4590 t
ORIGIN      
        1 attcgcgtaa ccagatgccg caggcatcgt gaattgcggt agcaatcgcc ggagctgcgc
       61 catttacacc aatttccgag atcgatttcg ccccgaacgg gccgacttta tcgtcgctcg
      121 gcaccaacac agcgcggaaa tcacgcggaa tgtcaccaat tttcggtgcg ccgtaactgc
      181 gtaaatcacg cgttaacggg tgaccttcgg cgttataaat gatctcttcg ctcatactgt
      241 ggccgatagc acgcagggtg gcaccgtaga tttgtcccaa cgctaactct ggattgaccg
      301 gtgtaccgca gtccagcaag gcgtagaatt tatccaggcg gatttcaccc gtacgcgtgt
      361 tgacggcaac ttcagcgaag tttgcgccat acgggaaagc gaaatcaggc gtgatataac
      421 tgccagttcc caccagtgaa ccaaagccgg tgccggtttc gcctttatgg gcaatatccc
      481 cgaaactaac ttcgcctttc ttgccgcgca cgacgcccgg cgttgctagt tgaacatctg
      541 ccactggctc acccaacatt tgcgcgccgt ggaacagaat tttctcccgc agattttccg
      601 ctgccaaacg cgccgcgtta cccgagaagc aagtaccgga cgaggcatat gcgcctttat
      661 caaacaacgc atgatcggta tcaccggaga taacatgcac gtcctgcggt gggcagtgca
      721 gcacttctgc tgccagtttc gtcactacag tatccagacc agtaccaata tccgcaccgc
      781 cagaatgaac gataaaggtg ccgtccgatt ccagtttgat catgcagtta gcctgatcga
      841 tatccgggat acccgatttc tgcatgataa tggcgacgcc gcgaccgatg tgccagtcac
      901 tattttgcgg tttcggtgaa gaccattgga tcatctcgcg accctgacgc aggatctctt
      961 ccagtgcgca gctggcggcg gaaggaacgg aggtcggcgc tttaccttca ccgattgcac
     1021 cgagaatttt cagctcttgc ccttcgtgta cccggttacg ttcgataatt tccagttggt
     1081 cgatctgtaa ctgttcagcc agttccgcta atgccatggt gatagcgaag ttacctttcg
     1141 gtgcgccata accctgataa gcaccatttg ggcaaatgtt gctgtagtag gtggtgacct
     1201 ggaaatcgac gttatcgcac ggatataacg gcagcgacag cgccggtccg ttacacggta
     1261 cggtgagtga gtggttgccg taagggccag tgttggcgcg gaaatccatc ttcactgccg
     1321 tcaggcgacc atcttttttc gctcccagtt tgacggtgac tttcgcgacg tgacgagagg
     1381 tgttagcaat aaactcttct tcacgggtgt agcggaacag taccggacgc ccggtcacgc
     1441 aggttgccca tgcgcacact tcttccagca ggatgtcctg tttggagcca aaaccgccgc
     1501 caactcgctc tttaatgaca tgaactttat gctgtttcat gcctacgagg cgcgcgacct
     1561 ggcggcgtaa gtgccatggt acctgggtgg aggcgtggat aaccagacga tcgccgtcca
     1621 tccgggtaaa gcagatatgt gtttcagtcg ggcactgctg tgcctgcgtt gagttatagg
     1681 ttcgctcaat gatcacatcg gcatcggcaa agcctttgtc catatcgcca atatgaccat
     1741 gaatactggc ggcgatattt ttgcgagggc gagaaccgat cgggaagttg atgatcatat
     1801 gctcgccgcg ctgggctgca tggctgttat cgtcttccag agtatctggc gcaccagcaa
     1861 catacaccac cggttcatcg tgcacgacag gcgcatcttc cgccattgct tcgtcgatcg
     1921 acattaccgg cttaagcact tcatattcaa cgtcgatgag cttcaatgct tcgagcgcaa
     1981 tttcttcact ttctgcgacg accgcagcaa cacgatcgcc gacgtgacgc attttcttgc
     2041 cgaacatacg gcggtcaagc ggtgacggtt ccggtgcgct ctgaccaccc ggggtatagt
     2101 agatatccgg gcaattcagg tgagtaataa cgtgaacgac gcccggtaag gcttcagctt
     2161 tgctgacatc cagatgagta atcagtgcgt gagcgtgtgg gctacgtaac attttaatga
     2221 cgcaggcgtc agccgttacg cggtcttcaa catagcaggg tttcgcctgc accattttcg
     2281 cggcatcagt tttaggataa tgcttgccaa tgacttcaag gtcgtcacgg aaagtcggag
     2341 cgatatcaat ggtggcctgc ggattatttt tacgtgcaac cgccagttca atgacctgat
     2401 agtattgttg ccagcctgca tcgcggctga acaaaccaga aagcgcgtcg tcgatctctt
     2461 cgcgggtagg tgcggcgatg cgatcgagaa gatcggtgat aatcagagct gcagctggat
     2521 cgttataacc agactgcacc acgccaacat caaccattgc ctgttgaacc agacttaact
     2581 cgttccattt gcccaaagat tctgcggtac gaatatctgc cttctctaac tgtgcggcaa
     2641 taagcaagga cgcgttaacg atattaccgt taaagattat tgcgtcagaa ccggcaaacc
     2701 cgaagccatc atcactgttg cgtacagagt gcattcccat gttaaacaac agcttttgca
     2761 cgttttcgcc tggattaacg gttagctcct gaggcgcgcc atttaaagta aagtggatga
     2821 tcatacggct tcctccccag cctgttggca gtcggcatac aggtcggcta ccagaactcc
     2881 cgtgatatag cgtttatagg ccacgctgcc ccgcaaatct tcctgcggga aaatggcgtt
     2941 ggcgacagcc tgttcaagtg cattgccttc cagattttgt ttttcgacat catgcagacg
     3001 cagtgcttta ctggcgacgc catccagcgc aattcgcata ccgtcgtggt ctgtcattgc
     3061 aacggctgcc gtcacgacgg ttaaacctgc ctgagaacgg ctaattttgc gggtcgcaca
     3121 ggtgcgatac ggatctttaa tgataatttc ggttagcagg cgatcgcatg ggcaggccag
     3181 gtagtcctcg attgacagcg tttcgccgtt gccaaaaacc agttcagcat ccagtgccaa
     3241 caggacggga agcagcaccg actcttcctg gcgggcggcg atttcaccac caatggtcga
     3301 ctgattacga acatggcgtg agtaaacaaa accgagggct tcacgcagcg ctgcaggaat
     3361 aaatcgcgca tcacgcagtg gctgcaagcg agacattgcg ccaatccgca gtgcaccgtt
     3421 atcccagtca acccagtcca gttccagatc ctgtaaggaa atggcaatct ttttatcggt
     3481 acgggttggt gtagcattga gtttgctgcc cccggcgaac cagacggctt catcctggta
     3541 gcggcgcttc agttccagcg cctgttcgac agagtcgggc ctgaaaaatt gttcaatcat
     3601 cttctttcct tatttaaccc tcttccctgc gggaagaggg catctgtcat ttatgccagc
     3661 gcatccatcc gacgccacat actggcagcg gcttttctgg cttgtgcata aatggaatcg
     3721 caatcgaagt taaactgacg gtcttcatag accatcacac cattgaccat cacgctgtga
     3781 acgctgcctg agcccatacc gaaagcgata tgcccggcga tattgtctgc cagcagcggc
     3841 gtcggtgagt tgtaatcgca aatggttaaa tcagctttgt aaccggcttc cagaagtcca
     3901 aatttcgcgc caaagttgcg gctcatcagt tcgttaccgt tagtcagggc tttggcaaaa
     3961 ctgtcaggcc acagcggacc acccgcatcg cgatgtttaa agaaggcaaa tttcatctct
     4021 tcaaacatgt ccgaaccaat gccgtccgtt cccaacgcca gattgcggat gtcgctaagg
     4081 tgatggttgt agccgacatg gttgttcatg tttgaacggg cgttatgcac caggaacgca
     4141 tcgcgctgat tgagtagggc gatgtcatct ttcgacaagt acagcccatg agcgaccagc
     4201 gttttgctat cgatgagatc gaattgcgct agtcgtgcca gcaggtcttt gccgtaccag
     4261 tggtgactgt aggaaacgtc gtaaaggtct tccgcagcgt gaatatgcaa accacggcct
     4321 gtggctttca cggcttcacg tagcatctcc agaccggcat ctggcacggt aaacggcgcg
     4381 tgagcaccga tatgtgcttc caccagatac ggctcggttg ctgctttctt cgcctcatca
     4441 atctgacggg cgaagcggat gttttcttct acaccttcct gcaactcttt gatgccgttg
     4501 ttacggtcag tagtttcaaa acaggtcatc gcgcgcaggc caacttttaa aaatgcgtcg
     4561 cgcaatgtgg agagcgaccc gccgatatac gccggagagg catggtgatc gataaccgat
     4621 gtacatccgc tcttaatcgc ttccagggaa caaatcagcc cgctgtaata gagcgactct
     4681 tcatcaaggg cgcgatcgag ccgccaccag agatttttca gcgttgagat gaaatccggg
     4741 cagggggcga tgtttgccat aattccgcgg gaaagccccg agtaaaaatg gttgtgcgag
     4801 cagacaattc ccggcatcac aatccgacca tgcatctctt tgtagctggc atcggggtag
     4861 cgttgcgtca gggcatcgcc gatagcgaca atcacatcat tttcgatagc gatatcaacg
     4921 ccttcctgca ctttcgccgg gtgtaactgc actgcggtga cattcttcag aatcaacatg
     4981 attacacctc cacgcggccc agcagataat gatgatgctg gtggacatgg ctgatgatgc
     5041 ggcacatatc gttcagctcc ggtggtacgt tgttgaactg accttcgctg tcgatgttta
     5101 acacccagct ttggttattc agtcgtactc gtacccggca atcttccaca aggaagcctg
     5161 ggttgctgct gttatcaaag tcttgcgcca ggctgaaaac ggtgattttg tctttgtatg
     5221 gtttaccgtt ccacggacag aactgagcgc agttgccgca ttcgttacag taagcgtcga
     5281 ggtgcagcgt ctggaatcta ttctggaagc ctgggaccgc aatggagacg ttggcgcggt
     5341 tcgggcagac atccacacac ttgctgcaaa cgtagttaca ttcgaggcag cgagcggctt
     5401 cctgggcgac aaacgcgtca cgatcgtcac tgttcaccag agtgatagag atatcgcctt
     5461 tacgttgata gatttccgcc ggattgacgt tgttccagta tttatcgttc tggtgggaac
     5521 ggatattttc ccgactaagg atggcatcgg tcgcccgacg cgcggttccg acagcagcga
     5581 caatggagga tggtccgcgc tgtacgtcgc cgatcataaa gacgtcagtc agacgagttt
     5641 cgccattgtg gtcgacgtct ggccagccgt ttttgtccag cggcacgccc atcgcattca
     5701 gggcttcagt atcctgctgt tcaccaatgg cggtgatcag gctgtctaca tgcagtgtta
     5761 ctgtttcgtt ggtttcaacc ggacggcgac ggcctttctc atcgggttcg ccaagcgaca
     5821 taacgcgcaa ggttaaggtg ccatcagcat cgaaacgttc cggattattc aggaaacgga
     5881 actccacgcc gtcgtgcaac gcttcttcat actcttcgcg ccatgcgggc atctcttgca
     5941 gtgaacgacg gtaaacgatc gttgcttttt ctacgcctgg aacgcgtaac gccgcacgag
     6001 cgcagtccat tgcggtgtta cccgccccga caacgaccac atgtttgccc agcttgagcg
     6061 ctgtaccctt gttgtattca cgcaggaagg ggagtgattt ccaaacattt tggttgtcac
     6121 ccgccagttt cacaccgcta tttttatcag tgccggtggc aatcagaaca tagtggaagc
     6181 cctgattttt taactgttca acggtcaaat cgggtgagca gccatactca aatttcacgc
     6241 cgtgatcggc aacaaaatcg atatcgtgct gaattaactc agccggaatt cggaactgag
     6301 gaatgatatt tttcaccacg ccgcccgcat tggcttcgcg ttcaaacagc gtaaccggat
     6361 ggcccgctct ggcaaggaag taacctgctg ccagacccgc cggacctgca ccaatcacgg
     6421 caaccggatg gcgtgaacca gaaccggctg gtttgtgcca gcgttgctta tattcatccc
     6481 aacctttttc cagcgcgact tttttcagtt cgcggatatt cagcgcactg tcgtaatcca
     6541 ggcgggtaca gttgtattgg cactggtgat cgcaaatatg accggtaatg gcgggcagag
     6601 cgttgcgttg gtagatgagt tccagcgcgt cggcatagcg gtgttcgcca agcagacgga
     6661 tgtattccgg aatatcttgc ttgatagcgc aggcagtaac acagggggca acgtagcaat
     6721 cggtcagcgg caggtcttct gccacttcaa tacgctcttc tggcttccag tgtttctggg
     6781 tgtattccat ggttaacgca tctgctgcca gtctgttcag tcgttcgacg tcaacatggt
     6841 caagtcccca ggcgtcggag ccttccagct cgcgcatgca ggcacttaag cgcaaatagc
     6901 cgccaggttt cagcaggtcg gttgccatag taatagggcg aatacctgta tcaaaaatat
     6961 cgcggatagt cagctgactg gcaccaccgg aataagaaat gggcagtttg ccgtcaaagg
     7021 cgcgagagag aactgctgca acattgatgg agagcgggaa cagcgcacgg cctgacatat
     7081 acatctcttc accaggcagt gcgcctttat tgttgatggt gccgagagtg ttagtcagtt
     7141 ttacgccaaa gccgagtgat ttttcttttg ccagtgccat caggcgttcc agcatttcca
     7201 gtgcttgcgt cagcttgagg tcgtgatcaa atgactcttc ttttaagcct atgtaaccga
     7261 aaccgcagac atcgaggatc tcacgaacac gcgcgtatcc cagtaaggtc gggttaagtt
     7321 tcacaaaggt gttgagccct ttttcttcca gcatgtagcg gcaaatggct tcgatttcat
     7381 gcggaggaca gccgtgcatg gtggagaggg tgacgccctg caccatactg gtggggatgc
     7441 gagcgggtaa ggcttgcaag ctttcgcgtt tttcctgcaa tccgtggcgg gctaaaaatg
     7501 cgtcatcctg gagtaattta ttcagcgtat cgcgatattg cgcgaatttc ggatggtcag
     7561 atgcgtccat catattgtcg atgaactgtt gcattggcgg ttgcttaata ccttcgaggt
     7621 tgtaaccgac gctcatatta aagatgaacg atttaccgga atcagaaggc tggaacatcg
     7681 cttcgagaag gtgcagggca aaccaggctt tgaggtattc atcccaggct ttaagcaggg
     7741 taaattcggt agaccattcg gtgttaaagc actcgtcttc ggcatcgata cagggctttt
     7801 ccagctccag gcggtcaaga atttggacgg tttttagttc gatgaagcgt ccgccagtca
     7861 gccaggaagt gacaatattt tgcgcgagct gcgtgtgcgg gccagcggca gggccgacgg
     7921 gagtggcaca ggtttcaccg aagacgctaa cagttttacc ttttacaggt gagtaaaact
     7981 gttgctcggg aataccaaag attgagcgtt gttgttggta ttcatcaaat atgcgcgtca
     8041 aaagttcctc aaacggaatg ggacgcataa tatcccccat aaccctctcc ttagcttata
     8101 cagatatcat tgagtgaccg gtgttgagaa atccggtggt ttagggggat agagcaacaa
     8161 tcgcaccact cttgccaggt ttggcgggaa atataaaaaa atgtgaggcg gctcgaaact
     8221 taaaggcttt ttgtgaatga actcacaggt gccaaatgag aattaaaatc actcctgaaa
     8281 attattattg tcagcccatt tatcactttt tctcttttct caaaatcagt gattccttat
     8341 cattatgata aagagtgctg gagagattaa atatttgaac gcaatcgttt aactacattt
     8401 tctaaatttt agtatacctt ttttgcgctg taataaagtc gtccggagta tcaatatcta
     8461 aaattatttc tgcattttct atctcgacgg aataatgagc tcccataagg agtgcctgac
     8521 gcatattggt gacattgggt cgttggattg cctgcatcaa gcatggtttt gacactaaaa
     8581 tcggatggcc ggggatgcca ttatggagtg gcagtattgc gccatcattt cgtaacgacc
     8641 agatttttct aaaaatatcg atggtgaggg ttggcatgtc accgtgggtg agaaaacaat
     8701 gttctgtttg cactgcgggt actgcggcct ttactgatgt tagtaaaccc tgcgcataat
     8761 ctgggttgtg gataatagtg atattgctct ggttcgcata acgttcgtgc agttcattac
     8821 cacgatagcc ggtgactaaa ataattcggc tacaaaactg caacgcattt ttgatacttg
     8881 tatcaagaat tgtcccctgt tgccagggta acatcatttt ccattgcccc attcttgatg
     8941 ataatccggc agcggtaatt atacagtcga tggctgacat agtggttcca ttatttatag
     9001 gggctaataa cgcctgataa ttgcgttact tcctgatgga ttaagttggg gataatttat
     9061 cccagagagg tcataaagac tcgtgaaaag tatagttgac ccatcggcat tagtcattga
     9121 tttaggtgcg cagaaacgtc ccactgtgat ttctgttgtt ggtgctgggg ggaaaaccag
     9181 cttgcttttt tggctggcag aactgctcca ggctagcggc aggcgtgtgt taatcacgac
     9241 gacgacacat atgtttatgc caacgtctca ctggcccgtg gttttctgtc gtgatcccgc
     9301 catgcttcct catgcgtctc ttacatcccc catttcattt tgttttcaca gctggaaagc
     9361 gaaccaggga aaagtgcagg gatttacgcc agaagcgatt gatgcactgg tgcaacgacc
     9421 agagtgtgac gtaattctca ttgaagcaga tggctcgcgt ggaatgccgt taaaagcgcc
     9481 tgatgagcac gaaccttgca tacccaaaag cagttgctgc gtgattgctg tgatgggagg
     9541 acatactttg ggcgcgaaag tgagcacaga aaatgtccat cgctggtcgc agtttgctga
     9601 tattaccggg ttaacacctg atgcaacctt gcaactgagc gatctcgttg cgctggttcg
     9661 ccaccctcag ggggcgttta aaaacgtacc gcaaggttgt cggcgagtct ggttcattaa
     9721 ccgtttttct caatgtgaga atgcgattgc acaaagcgag ctccttcaac cgctgcaaca
     9781 acacgacgta gaggcaatct ggctgggcga tatacaagaa catcctgcaa tcgcgcgcag
     9841 atttgtgaat tagcggcccg ggtattccgg gttactgaaa ggatatccat tgaggttggt
     9901 atgaatattt tcacagaggc tgcaaaactc gaagagcaaa attgtccgtt tgcgatggcg
     9961 caaattgttg atagccgagg ctcgactccc cgccattctg cacaaatgtt agtgcgcgcc
    10021 gatggttcta tcgtcggtac aattggtggc ggaatggttg agcggaaggt gattgaagag
    10081 tcacttcagg cattgcagga acgtaagccg cgattattcc atggacgtat ggctcgtaac
    10141 ggtgcggatg ctgtcgggtc agattgtgga ggtgcaatgt cagtatttat cagcgtccat
    10201 ggtatgcgtc cacgtctggt gttgattggc gcggggcatg tcaaccgggc gatagcccag
    10261 agtgcggcgc tattaggatt tgatattgcc gttgccgata tttatcgcga aagcctcaat
    10321 cctgaactat tccctccatc aaccacgctt ctccatgctg agtcgtttgg tgcagcagtg
    10381 gaagcactgg atattcgccc tgataatttt gtcctgattg ccacgaataa tcaggatcgt
    10441 gaagccctcg acaaactcat tgaacagccc attgcatggt tggggttgct ggcaagtcgc
    10501 cgcaaggttc agcttttcct gcgtcaattg cgtgagaaag gcgtggctga agaacatatt
    10561 gcccgtttac atgcgcccgt tggttacaac ataggtgcgg aaacgccgca ggagatcgcc
    10621 atcagcgtgc tggcagaaat attacaggtg aaaaataacg cgccgggtgg gctgatgatg
    10681 aaaccttctc atccttccgg acaccagctg gtggtgattc gcggtgcggg agatatcgcc
    10741 agtggtgtgg cgctacgtct gtatcatgcg ggttttaaag tgatcatgct ggaagtggaa
    10801 aaaccgacag tgattcgttg taccgtggcg tttgcccagg ccgtgttcga tggcgaaatg
    10861 acggtcgaag gcgttactgc tcgcctggca accagtactg cggaagcgat gaaacttacc
    10921 gaacgcggat tcatccctgt gatggcagat cccgcctgtt cattgcttga tgaactgaaa
    10981 ccgctttgcg tggtggacgc tattctggcg aaacagaatt tgggaacgcg ggcagatatg
    11041 gcaccagtaa caatcgcgct tgggccgggc tttactgcag ggaaggattg tcatgcggta
    11101 attgaaacaa atcgcgggca ctggctcggt caggtgattt actctggttg tgcgcaggag
    11161 aataccggtg ttcctggcaa tattatgggg cataccaccc gacgggtgat ccgtgctcct
    11221 gctgcaggca ttatgcgatc caacgtgaaa ttaggcgatc tggtgaaaga gggcgatgtg
    11281 attgcctgga ttggtgagca tgaaattaaa gcaccgttga cggggatggt gcgtggcttg
    11341 ttgaacgacg gcctggcagt ggtcggtggt tttaaaattg gtgatatcga tcctcgtggt
    11401 gaaacggctg atttcaccag cgtttctgat aaagcccggg cgattggcgg cggcgtactt
    11461 gaggcgttaa tgatgttgat gcatcagggc gtgaaagcga caaaagaagt gctggaagtg
    11521 gcttaaatga aaatggccgg atggtattac ccacccggcc ttaaaattac tgacaaatca
    11581 ccgttccggt tttgccttca atcccctctt tcgctttgct caatacggta atcagtgctt
    11641 cacgacctgc gcgagagcga gcaaacgagg cggcagcttc cactttaggc agcatagaac
    11701 ctttcgcaaa atgaccttct tcgataaagc gttcagcatc acttaatgac aggcgatcga
    11761 gccactgttc attctcttta ccgaagttaa tggccacttt ttctaccgca gtcagaatga
    11821 tcagcatatc ggcatcaatc atttctgcta aacgggcgct ggcccagtct ttatcgataa
    11881 ccgcgctggc accacgcaga tggttacctt cacgaataac aggaatacca ccaccgccaa
    11941 cggtgatcac cacctggcct gcatctagca gagctttaac cgtttctttt tcaataatat
    12001 caaccggttt tggcgaggca acaacacggc gatagccgcg accagcatct tctttcagag
    12061 tgtaaccctg ttttgtcagt tgctctgctt cttgctcggt aaagaacgaa ccgatcggct
    12121 tggtcgggtt gaggaatgcc ggatcgttag cgtcgacttc gacctgagta accagcgttg
    12181 ctaccggttt attaatgccc cgagaaagca gttcttctct taaggcgttt tgtagatcgt
    12241 aaccaatata accctggctt aaggcaacgc agacagacat cggcagcatt ggcgagtgcg
    12301 cttcagtttt tgcagcagct tcaaaggcct ggttaatcat gcccacctgc ggcccgttac
    12361 catgagtgac gataacttca tgtccttggg caattaaatc aacaattgcc tgagaagtaa
    12421 tttttaccgc tttcatttgt ccggccaggt catcgcccag cgcattcccg cccagggcga
    12481 gaacaatttt cttactcatt tttcctcact catcattctt gattgtgtat agatccctga
    12541 cagtggttag agcacgggag ggacaaacgg tttgcgtcgc aggaaacgac ctcgcccggc
    12601 tttgcctgta aaggtgccgt cacagaaaat cgtttcacca cgggataagg ttctgacaat
    12661 cgcgccctga caggtaaaac cctcccatgg cgagtagtcg gcgttgtcgt ggagatggcg
    12721 atgctgaatt tgttggctct gacgtgggtc gataatcacc acgtcgccat cggaaccggg
    12781 cgctaataat cctttttgcg gccacaggcc aaacagcctg gcgggcattg cgctggttaa
    12841 ttcaacaaag cgttccggtg ttatacgtcc cgtcatcacg ccactggaaa acagtaactg
    12901 catgcggttt tccacaccgg gtaagccatt tgggcagcga ctgaaatcgc ctttagaaat
    12961 ttgcaggcgt tgagccatcg agaaggtgca gtgatcggtc gccaccacgt caatcgcacc
    13021 atcgctgatg ccacaccaca gtttgtcctg ctcgcgtacg ttacgcagcg gtgggctaag
    13081 aatgaacttc atgccgtctt ctgtatcgta actgcgttcg tccaacagga gatattgtgg
    13141 gcaggtttca acccagactg gctggtgatt cgcacgggca agacgcagat aatccagacc
    13201 taagccgtta gacaggtgca cgatatagag cggggcgtta ccggcaattt gtgccaggtt
    13261 aatcatgcgg gcgatggctt ccgcttcgca ttccagaggg cgactcaagg catgatagcg
    13321 cggcgcggtt aaccctgcgg cgataaattc cgcccgcttg ctggcgatag ccgcatcatt
    13381 ttccgggtgc acggtggtca gcgcgccgga ttcatgcaga cggcgtaatg cctgcaaaac
    13441 ctcgtcatcg ttgagtttgt attgataggt taaatagagt ttaaaactgc tcagtccttc
    13501 ctcgaccatc atcgggattt cgtcgaggat tgcgtgatta atgtgctgga tcacaccgtg
    13561 aaagctgtaa tcgatgaccg ctttatgggc ggcataacca cgataaacct ccagttgatg
    13621 gcgtaaccga cagccgtttg ggccaaatcc catatggtca ataatggttg ttgtaccgcc
    13681 acacgcagct gcgcgggtac cggtaaaaaa atcatcacaa ctgcgcgcga tgccgacatc
    13741 aatattgaaa tgcgtatgga catccacgcc gccagggaaa acgtaacagc cagtggcatc
    13801 aatttcttca tacgggagct gcggcgaaat attgttgccc aactggcgaa caatcccgct
    13861 ttcaatcagc aaatcctgtt tggcttgtcc atctgcgtta acgacagtgc cgtttttgat
    13921 caatacgcgc atagcaaact ccaaaaaccc tccggcgaac cggaggggaa taagtgaaga
    13981 gtaattactc tgttgccagc cagcttaacg ggattgcagc gtacatcgcg gcacaggtca
    14041 ccaggtgaga tttccaggtt ttttcgttag gtgcatgcgc ttcaggttct ttacccgggc
    14101 caaagccgat cactggaatg ccgtgacggc ccatgataga tacgccgtta gttgagaagg
    14161 tccacttatc aacaaccggc gctttgccaa acagcccttc gtaggcattc accagtgctt
    14221 taacggtgaa gtgatcttct tccactttcc aggtcgggaa gtagcattcg gttgggtaaa
    14281 ccaggccagt ccaggacgga cggtcgtagt tgtacataga aacaaccgcg ttagctttct
    14341 gtactgcagg cagggcgcgg atttcgtcca gcgcgccttc ccaggtttcg ccccaggtca
    14401 gacggcggtc aatagagacg gcgcagctat ctgctacagc gcaacggctt ggggatgtga
    14461 agaagatttc agaaacggtg agggtgcctt tgccgaggaa ttcgtcataa cccagacgtt
    14521 gggagagttc ttgtaattcg ccaagaatcg gacccatttt gaaaatggcg ttgtcaccgc
    14581 gttctggcgc agaaccgtgg cagctaacac cctgaacatc aatacgaatt tccatacgac
    14641 cgcgctgacc acggtatatc tggcagtcgg ttggttcggt actgaccaca aattccgggc
    14701 gaatgccgga ttgttcaata atgtactgcc agcacagacc gtcgcagtct tcttcctgca
    14761 cagtaccggt aaccagcagg gtatattcat cttccagacc gaggtcttta atgattttac
    14821 cggcataaac catagatgcc atgccgcctt cctggtcgga agtaccgcga ccaccgatta
    14881 gctcatcagt ttccatgcct tcgtacggat cgaagtccca gtttttgatg ttgccaatgc
    14941 cgacggtatc gatatgagcg tccattgcca ccagacgcgg gccgtggccg atataaccga
    15001 gaacgttgcc catcgggtcg atttcaactt tatcgaagcc gactttttcc atctcttctt
    15061 taatacgatg tactacgcgt ttttcgtcgc agctttcact gggaatagca accatgtcgc
    15121 gcaggaagcg agtcatgtcc gcctggtaat cttttgcttt ttcaagaatc agtttgaatg
    15181 gaatattctt agccattatt tttctccaga ttttttactc cccgttttgc agggagtagt
    15241 tccaatagag tgattaaggt gctacagcgt gtttgccttc ccagacaact tcgcggtagt
    15301 gcttcacgtc ggtgtcgcct tcagtgctga taaccagcac cacggcatct ttgttcagcg
    15361 ccagtttttc catcaggctt tgacgttgcg ggtgataatg aaccgctgcg agaacgccca
    15421 aaccgacagc gccggattca ccggagatga tgcgcgggtc gttgccgtac ggattaccca
    15481 gcacgcgcat acctaatgcg gcaacgctgt cctggcagga gatgaattgg gtggcgcagt
    15541 tacgtaggat ttcccagccc agcgggttag gttcgccaca ggccaggcct gccatgatgg
    15601 tggccatatc accgccaacg ttgacgatgt cgcctttgac gccggagcga taaatacagt
    15661 cagctttgtc aggttcaaca ataatgctgt gcagattttg cgggctatag acgtcgacca
    15721 gataacccag cacaccaccg gccattgctc cgacaccggc ttgcagcaga acgtgcgtcg
    15781 gggttacgcc catttcacgc atttgctcga cggcttcatc tgccagggtt gcgtagcctt
    15841 gcatgatcca ggttgggatt ttggtgtaac cttcccatgc cgtgtcctgt accacttccc
    15901 agccgtgctg ctgcgcgtgt tgcatggtca ggcgaacggt atcgtcatag ttcatatctg
    15961 tgacgatgca ctcggcaccg aggttcagaa tggcgtcaac gcgttcctga gcagaacctt
    16021 tcggcatgta aatcaccgca ttctgtccga gttgctgcgc tgcccacgcc acaccgcgcc
    16081 cgtggttgcc gtcggtggtg gtcgcgaaag tcattttttc gccgatggca tttttcaggt
    16141 gctcaaatga cagcgtttcg atatcaagat gatatttttc acacaataat tgagcgatag
    16201 cgtacgcacc gccgagcatt ttgaaggcgt tcagaccgaa tcgttttgat tcgtctttga
    16261 caagaatttt cttcacccca aaaaggtttg cgagatcgtc cagcgcacaa agcggtgttg
    16321 ggcgataacc agctattttc tggtggaact ggcgcgccag tttggcctgg ctttgcgaaa
    16381 agagcggtga tgtttcgccg ttgaaaaatt tgttatcggc gatatcaatc ttcaatgaga
    16441 aaacggacat atcctatcct tttggcctct ggaaatagag ggccgaccag ctgatcggcc
    16501 cgaggggtta tttcacgcgt tcttgcgctt ctttcagcag ttgctcgagc agtgcacctg
    16561 gtttggcgta tttacgggac aggatcatcg cggcgatgat ataaggcttc cagctggctt
    16621 ctttgtaggt agcgatacgg tatttttcga atacgccttc agtcacttcc ccttctttgc
    16681 aggatacgcc gctgatatca gctggcaggc agtgcatgta cagggcttcg ccatcacggg
    16741 tcagttccat catctcttca gtacaatgcc agtctttgtg ttgcgcgttc tgtgccagac
    16801 actgtttttc cagtgctttt aagccttcgt gatcgttagc gcgcagcaat tcggtacgct
    16861 gttccatcac tttgtaaggt gcccatgact tcggataaac gatgtctgca tctttgaacg
    16921 cttcttccat gctggtgacc tgacggaagc taccgccaga tgctttagca ttgtttttcg
    16981 ccacttcaac cacatccggg atcaggtcgt agccttccgg atgggccagg gtgacatcca
    17041 taccgaagcg agtcatcaga ccgatgatgc cttgtggtac agagagcggt ttgccatagc
    17101 ttggagagta ggcccaggtc atggcgattt ttttaccttt caggttttcc agtgaaccaa
    17161 agtgttcacg taaccacgcg aggtcagcca ttgactgagt cgggtggtca atatcgcatt
    17221 gcaggttcac taaagccgga cgctgtggca gtacaccctg cttgtaaccg tcatcaagtg
    17281 cagcgccaac ttcacgcata taggcgttgc ctgcgcccag atacatatcg tcgcgaatac
    17341 caatagcgtc ggcgcagaag gagatcatat tggcggtttc acgcacggtt tcgccgtgag
    17401 cgatttgtga tttgccttcg tcgagatctt gttgtgcaag gccgagcagg ttaagcgcgg
    17461 aagcataaga gaagcgggta cgggtggagt tgtcgcggaa tacggaaata cctaatccac
    17521 tattaaagac tttggttgag atgttttcag cacgcagtgc ttttaatgct gcggcaacgt
    17581 ccagtacttg tttcagttca tctggcgtct gttcccacgt taacaaaaaa tctttctcgt
    17641 gaaggtgaga ggtcagcgaa ttgatatcct taatcagctc attaacagtt ttcatcataa
    17701 agtcccggta gtggaatgac aggcaaagtg gtttatttca ctgtgacttg ccaaagtgat
    17761 tggtgttttc acagctatac ctgggactaa caataagtgt gccagaatta tccacggggc
    17821 tgcaaaagac taatttgacg gataatgtgt ggggttgatc acaaattgat gccagcttcc
    17881 ggttgtttgt aaaatacgaa aacgtttggc tttttttatc gcgttattta tttgctgcta
    17941 agtatcagaa ataagataaa gggacaggct atctctcttc agtttgataa actcaccgcc
    18001 gtaattctca ta
//
LOCUS       DAZGLJ010000087        17990 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000087 DAZGLJ010000000
VERSION     DAZGLJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.087"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            144..710
                     /gene="yqaB"
                     /locus_tag="ACHK49_002547"
     CDS             144..710
                     /gene="yqaB"
                     /locus_tag="ACHK49_002547"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO9263176.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKYHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            707..1135
                     /locus_tag="ACHK49_002548"
     CDS             707..1135
                     /locus_tag="ACHK49_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO9263177.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1208..2764
                     /gene="gshA"
                     /locus_tag="ACHK49_002549"
     CDS             1208..2764
                     /gene="gshA"
                     /locus_tag="ACHK49_002549"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO9263178.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            2914..3429
                     /gene="luxS"
                     /locus_tag="ACHK49_002550"
     CDS             2914..3429
                     /gene="luxS"
                     /locus_tag="ACHK49_002550"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO9263179.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGKGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3493..5031)
                     /gene="emrB"
                     /locus_tag="ACHK49_002551"
     CDS             complement(3493..5031)
                     /gene="emrB"
                     /locus_tag="ACHK49_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO9263180.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5048..6220)
                     /gene="emrA"
                     /locus_tag="ACHK49_002552"
     CDS             complement(5048..6220)
                     /gene="emrA"
                     /locus_tag="ACHK49_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO9263181.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6347..6877)
                     /gene="emrR"
                     /locus_tag="ACHK49_002553"
     CDS             complement(6347..6877)
                     /gene="emrR"
                     /locus_tag="ACHK49_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO9263182.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(6968..7303)
                     /gene="ygaH"
                     /locus_tag="ACHK49_002554"
     CDS             complement(6968..7303)
                     /gene="ygaH"
                     /locus_tag="ACHK49_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO9263183.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7293..8030)
                     /gene="ygaZ"
                     /locus_tag="ACHK49_002555"
     CDS             complement(7293..8030)
                     /gene="ygaZ"
                     /locus_tag="ACHK49_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO9263184.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8154..9338)
                     /locus_tag="ACHK49_002556"
     CDS             complement(8154..9338)
                     /locus_tag="ACHK49_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9263185.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     LLGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFINDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9530..10522)
                     /gene="proX"
                     /locus_tag="ACHK49_002557"
     CDS             complement(9530..10522)
                     /gene="proX"
                     /locus_tag="ACHK49_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO9263186.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10580..11644)
                     /gene="proW"
                     /locus_tag="ACHK49_002558"
     CDS             complement(10580..11644)
                     /gene="proW"
                     /locus_tag="ACHK49_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO9263187.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11637..12839)
                     /gene="proV"
                     /locus_tag="ACHK49_002559"
     CDS             complement(11637..12839)
                     /gene="proV"
                     /locus_tag="ACHK49_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO9263188.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13194..14153)
                     /gene="nrdF"
                     /locus_tag="ACHK49_002560"
     CDS             complement(13194..14153)
                     /gene="nrdF"
                     /locus_tag="ACHK49_002560"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO9263189.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14163..16307)
                     /gene="nrdE"
                     /locus_tag="ACHK49_002561"
     CDS             complement(14163..16307)
                     /gene="nrdE"
                     /locus_tag="ACHK49_002561"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO9263190.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYPNQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16280..16690)
                     /gene="nrdI"
                     /locus_tag="ACHK49_002562"
     CDS             complement(16280..16690)
                     /gene="nrdI"
                     /locus_tag="ACHK49_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO9263191.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(16687..16932)
                     /gene="nrdH"
                     /locus_tag="ACHK49_002563"
     CDS             complement(16687..16932)
                     /gene="nrdH"
                     /locus_tag="ACHK49_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO9263192.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17180..17509)
                     /locus_tag="ACHK49_002564"
     CDS             complement(17180..17509)
                     /locus_tag="ACHK49_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO9263193.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            17661..>17990
                     /locus_tag="ACHK49_002565"
     CDS             17661..>17990
                     /locus_tag="ACHK49_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO9263194.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLF"
BASE COUNT         4471 a         4574 c         4678 g         4267 t
ORIGIN      
        1 ctcccaaaaa tttccttcaa aaatatctca tttctaaata actactttat ccatttcctg
       61 aattttcttc tccttcgcca tcagcgacaa aatcaagcaa ttacgctaaa gtagcgtcat
      121 atttttcgac ttgcgagaag accatgtacg agcgttatgc aggtttaatt tttgatatgg
      181 atggcacaat cctggatacg gagcctacgc accgtaaagc gtggcgcgaa gtattagggc
      241 actacggtct tcagtacgat gttcaggcaa tgattgcgct taatggatcg cccacctggc
      301 gtattgctca ggcaattatt gagctgaatc aggccgatct cgacccgcat gcgttagcgc
      361 gtgaaaaaac agaagcagta agaagtatgc tgctggatag tgtcgagccg cttcctcttg
      421 ttgaagtggt gaaaagttgg catggtcgtc gcccaatggc tgtaggaacg gggagtgaaa
      481 gcgccatcgc tgaggcattg ctggcgcacc tgggattacg ccgttatttt gacgccgtcg
      541 tcgctgctga tcacgtcaaa taccataaac ccgcgccaga cacatttttg ttgtgcgcgc
      601 agcgtatggg cgtgcaaccg acgcagtgtg tggtctttga agatgccgat ttcggtattc
      661 aggcggcccg tgcagcaggc atggacgccg tggatgttcg cttgctgtga gtgaagcgtt
      721 atcgcttttc tcgttgtttg ccagtagttt tctcagcgct acactattac ccggcaactc
      781 cgaagtcgtg ctggtggcaa tgttgctttc cgggatcagt tatccctggg ttttagtttt
      841 aacagcaaca atgggtaata gccttggagg gttaactaac gttatccttg ggcgtttctt
      901 tccattgcgt aaaacatcgc gctggcaaga gaaagctacc ggctggctga aacgctatgg
      961 tgcagtcacg ctattattaa gctggatgcc cgtggttggc gatttactgt gtctgttagc
     1021 gggatggatg cgcatctcct gggggccagt aatctttttt ttgtgccttg gtaaagcgtt
     1081 acgctatgtt gcagttgcag cagcgaccgt tcagggcatg atgtggtggc actaattgta
     1141 ggcctgcaca tatggtcacc attacagtta tgctaattaa aacgattttg acaggcggga
     1201 ggtcaatttg atcccggacg tatcacaggc gctggcctgg ctggaaaaac atcctcaggc
     1261 gttaaagggg atacagcgtg ggctggagcg cgaaactttg cgtgttaatg ctgatggcac
     1321 actggcaaca acaggtcatc ctgaagcatt aggttccgca ctgacgcaca aatggattac
     1381 taccgatttt gcggaagcat tgctggaatt cattacacca gtggatggtg atattgaaca
     1441 tatgttgacc tttatgcgcg atctgcatcg ttatacggcg cgcaatatgg gcgatgagcg
     1501 gatgtggccg ttaagtatgc catgctacat cgcagaaggt caggacatcg aactggcaca
     1561 gtacggcact tctaacaccg gacgctttaa aacgctgtat cgtgaagggc tgaaaaatcg
     1621 ctacggcgcg ctgatgcaaa ccatttccgg cgtgcactac aatttctctt tgccaatggc
     1681 attctggcaa gcgaagtgcg gtgatatctc gggcgctgat gccaaagaga aaatttctgc
     1741 gggctatttc cgcgttatcc gcaattacta tcgtttcggt tgggtcattc cttatctgtt
     1801 tggtgcatct ccggcgattt gttcttcttt cctgcaagga aaaccaacgt cgctgccgtt
     1861 tgagaaaacc gagtgcggta tgtattacct gccgtatgcg acctctcttc gtttgagcga
     1921 tctcggctat accaataaat cgcaaagcaa tcttggtatt accttcaacg atctttacga
     1981 gtacgtagcg ggccttaaac aggcaatcaa aacgccatcg gaagagtacg cgaagattgg
     2041 tattgagaaa gacggtaaga ggctgcaaat caatagcaac gtgctgcaga ttgaaaacga
     2101 actgtacgcg ccgattcgtc caaaacgcgt tacccgcagc ggcgagtcac cttctgatgc
     2161 gctgttacgt ggcggcattg aatatattga agtgcgttcg ctggacatca acccgttctc
     2221 gccgattggt gtagatgaac agcaggtgcg attcctcgac ctgtttatgg tctggtgtgc
     2281 gctggctgat gcaccggaaa tgagcagtag cgaacttgcc tgtacgcgcg ttaactggaa
     2341 ccgggtgatc cttgaaggtc gcaaaccggg cctgacgctg ggtatcggct gcgaaaccgc
     2401 gcagttcccg ttactgcagg tgggtaaaga tctgttccgc gatctgaaac gcgtcgcgca
     2461 aacgctggat agcattaacg gcggcgaagc gtatcagaaa gtgtgtgatg aactggttgc
     2521 ctgcttcgat aatcccgatc tgactttctc tgcccgtatc ttaaggtcta tgattgatac
     2581 tggtattggc ggaacaggca aagcgtttgc agaagcctac cgtaatctgc tgcgtgaaga
     2641 gccgctggaa attctgcgcg aagaggattt tgtagccgag cgcgaggcat ctgaacgccg
     2701 tcagcaggaa atggaaaccg ctgataccga accgtttgcg gtgtggctgg aaaaacacgc
     2761 ctgacagaaa agaaaaaggc cactcgtgag tggccaaaat ttcatctctg aattcaggga
     2821 tgatgataac aaatgcgcgt ctttcatata ctcagactcg cctgggaaga aagagttcag
     2881 aaaattttaa aaaaaattac cggaggtggc taaatgccgt tgttagatag cttcacagtc
     2941 gatcataccc ggatggaagc gcctgcagtt cgggtggcga aaacaatgaa caccccgcat
     3001 ggcgacgcaa tcaccgtgtt cgatctgcgc ttctgcgtgc cgaacaaaga agtgatgcca
     3061 gaaagaggga tccataccct ggagcacctg tttgctggtt ttatgcgtaa ccatcttaac
     3121 ggtaagggtg tagagattat cgatatctcg ccaatgggct gccgcaccgg tttttatatg
     3181 agtctgattg gtacgccaga tgagcagcgt gttgctgatg cctggaaagc ggcaatggaa
     3241 gacgtgctga aagtgcagga tcagaatcag atcccggaac tgaacgtcta ccagtgtggc
     3301 acttaccaga tgcactcgtt gcaggaagcg caggatattg cgcgtagcat tctggaacgt
     3361 gacgtacgca tcaacagcaa cgaagaactg gcactgccga aagagaagtt gcaggaactg
     3421 cacatctagt cagtaaacta tcttcacaat taattgaaaa aagccagttc aaatgaactg
     3481 gcttagttgt acttagtgcg caccgcctcc gccgccacct gcgccaaatg gcggtttagc
     3541 aaaccacacc agccccagca gtacgaggaa tattccggct gacatccaga agatctcatt
     3601 ggcggaaata atcagcccct gattggtgat ctgctgggca atccagcctg atgcctgctg
     3661 ttgcgtcatc ccaagccctt ccagttgact gtacatcgcc tgggcattcg ggttgaacgg
     3721 gtttaccgac tcagtcaact gcgcatggtg catcgactcg cggttggtcc acatggtcgt
     3781 ggttatcgac gtgccgatag accccgccag cgttcgcgta aagttagaga ggctcgatgc
     3841 cgccgccagt cgttccggtg gcaaaccaga cagcgtaatg gtggtcagcg gcataaagaa
     3901 gcaggccacc gcaaacccct ggataaactg cggccaggcc gacgcgccaa aatccatacc
     3961 tggttcaaag gtataggcac gccagtagaa gcagacggca tacataataa agctgaaggt
     4021 taccagccgc cgcatatcca gtttatgcgc gaagcggccg ataatcggcg acaggatcac
     4081 cggaataatc cctaccggcg cagaggccaa acctgcccag gtcgccgtgt aaccgtagac
     4141 ctcctgcaac aactgcggca gcagaacaat agcgccgaag tagagcatat acgcgaggct
     4201 gatacacaag cagccgatgg tgaagttgcg cgacttaaac aacgacagat cgactatcgg
     4261 gttatcgtcg gtcagctccc agacaatcag gaagcagata gccaccaccg ccaccacggt
     4321 aaggatgata atttcctgtg atgaaaacca atccagctct ttaccgcggt caagcataat
     4381 ctgcaggctg ccgataccaa taaccagcag tgccagcccc acggcatcaa tccgccgccg
     4441 ttcggtgcgg gtttcacgtc cgcgcagagt ttgcagtgtc atcaacacca ccgccacgcc
     4501 aatcggcacg ttgatgaaga atatccagcc ccagtggtaa ttatcgctga tataaccgcc
     4561 gaggatcggg ccgcaaattg gcgcgacaat caccgtcatc gaccacaacg ccagcgcgat
     4621 cgagcgtttg gctggcgggt agttattcag caatagactt tgcgaaagcg ggatcaacgg
     4681 cccggcgaca atcccctgaa tcacgcggaa gaagatcagc atattcaggc tgctggagac
     4741 accacacgcc cacgacgcaa tagcaaaggc gatggtggac caaaggaaca gtttcacttc
     4801 cccgacgcgc tttgccagcc agccggtaag cgggatcgag atggcattcg ccaccccgaa
     4861 agaagtgatt acccacgttc cctggctgag cgatgagccc agattcccgg cgatagtggg
     4921 gatcgccacg ttagcaatgg tggagtccag cacctgcatg aatgtcgcca gtgacagcgc
     4981 aatcgtcata atgaccagtt gcgcgccttc cagcggtttt tgctgttgca tcacacgcac
     5041 ctctggatta gccagcgtta gcttttacga tatcgtcgat cagtttattg acaggtgcca
     5101 ggctgatttc acgcgcggta cttaccgcga ccggtgttga acgtacttta tttgccagta
     5161 cctgaccgtc acggttagtg gtattgacgc tcaccagcgt ggacaaacca atgcgcagcg
     5221 ggtattgctc cagctgtttc tggtccagtt cgatacgcac aggtagacgc tgaacgactt
     5281 tgatccagtt accggtcgca ttttgcgccg gaagcagtga gaatgcgcta cctgtgccca
     5341 tatccagacc aaccacttta ccggtgtatt tcacatcatc gccgtaaata tccgtggtga
     5401 tagtgaccgg ctgaccgata cgcatattgg caatctgcgt ctctttaaag ttggcatcca
     5461 cccacatatt ggtggctgga acgaccgcca tcagcggcgt cgttgggcta atttgcgccc
     5521 caggctgtac cgcgcggcgg gagacataac cggtcatcgg actgacaata cgggtacgct
     5581 ccagcgccag ccaggcgtta cgtacttcgg tggcagcctg ttgcacggca ggctgatctt
     5641 ccagtttagt ccccagaatc atcgcctgat tggcattgta ttgttgaata gcgacgtcca
     5701 gttgcgcctg ggcactggtg acggcgtcgc gggcgtgttg cagctcttcg cgaccaatca
     5761 ggttggcatt gcccagcggc acacggcggt tgtagtcgct ttgtgctttc gcgagggcga
     5821 ttttttgcac ctcaatattc gcctgcaact gtttgctgtt aatcatcagc tggtgggttt
     5881 ggcgaacgct ggaagccagt gcagttttgg ctttttcaaa cgcctggcga gcatctgtcg
     5941 ggtcgagagt gaccagaaca tcgccttctt ttacaaaatc ggtgttatcg gcccagactt
     6001 tcgtcacgct gccagacacc tgagacataa tttgaatctg attccctgcc acgtatgcgt
     6061 catcggtttc ttcgaaatga cgcagtacca aaaaccaata aatccctatc gctacggcaa
     6121 taattataaa gagcaaggtg agaaggagga gcagacgctt acgtttgccg ctcttcttta
     6181 ccggttgctg cggggtttga gtctccgcat ttgcgctcat attgttctcc acgatcttct
     6241 taactcatcg gctgagccga gctgcttgcc aaaaaggcca gcatgttgcg atgctggccg
     6301 gtcatttttt cttttataaa tctggatttt tgagcgagat gacgcgttag ctcatcgctt
     6361 cgagaaccac accgtcttgt tccatctggt cgagacggga gagcaatttg cgggtgattt
     6421 gctcgagctg atctttttct gttgtgctga gcgcggacca gagttgatgc aggcagttat
     6481 gctgcggtgg taaaacctcg cgcaaaaact cgtgaccttt ttccgttaat tgcagatgca
     6541 ggcagcggcg atcgttatcg ctttcacgac gttcgatcca accgcgtttt tccagttcat
     6601 cggcaatacg cgtcgcgttg gtacgggatg atccaagagc acaacttaat tcagaaggct
     6661 gaatactgtg gttttcctga gactccagcg taatcaacgc cataaacaac gtctcgttaa
     6721 tcccctgagc cttcagcatt ttattgcggt tctccagcag cttgctttgc atgtgcatgc
     6781 aaagacgagt cagaaggatc tcctgataag gaaaatcttc gtggcggctg gcgcgaaatt
     6841 ttagcatttg ttcaatgggc gtaaacgaac tatccatttg ggtatgacct cattaatttc
     6901 agccgatata gtaacgacag tgacaaataa agtaaatgta ttgttttaga aaaatgattc
     6961 ttgtgggtta tataatcgcc atcactttcc aggcgagccc ataggccagc gcactaagca
     7021 gtgttgggat gataatgctg cgtgttttat agaaactggc acccagtacc gcgaagccga
     7081 ccagcgtggg cacgaaacgg cgtgtatcgt gcatcacttc tggtgcggta gagacaacca
     7141 gcagagcgca tatcgaggcg atgccaatgg tgtcgagcaa aatacctacc gcgccacgtt
     7201 tggttgggcg ggcattaccc acacgcaggc gcagcggcaa atagcggaag caataattcg
     7261 ccacgccaac cagtaaccca agcagcagaa cctcatagct catcgggcgc tccttgccag
     7321 aatgcctgga ttaacgcagt gaggcagcca cagacaatgc ctgccagaat ggcgacggga
     7381 atagaaaata gcgttacgcc tgcaagggca ccaactaacg ctgcggtaac gcaaagagat
     7441 tgtttgcgct ggaaagaggc gagcaggaaa ctcataaaga gtgccggaag cataaaacct
     7501 aatgcagctt caacggcggg ataaccttgc agcaagccgc tgccggagaa tgcccctatt
     7561 accgtaccaa atacccacga tgaccatgaa ctgaaggcaa tgccgatcat ccagttctcg
     7621 ctccagcggc gattattgcg taccagtttt gcggttgcgg cggcaaaaac ctcatccgtc
     7681 aggccaaatg cccacagggc ggttttcgat ttttgcagac gctgaataat acggctacgc
     7741 agtgacgggc catacaacac atggcgaaca tccattgcca tgacggtcag tgcagcaacc
     7801 cacaaactac tcccggctgc cagcatcgcg gtaatgacga actggctcgc gcctgcataa
     7861 atgatgcagg agaaaaaaac gctttcgaga ggagagaatc ccagacgggt cgcattcaga
     7921 ccgaacgcaa aggccaccgg aatataacta ataacaatcg gtaaactgtc tttgcatcct
     7981 tccatgaagg tcgccgaacc aggagcaggc tgtggagtag ggctttccat agagtgtacg
     8041 cttaacgatt gttaatatta taaatagact gaatgaatat cttaacctta tcagactgat
     8101 gggcttctta acacccttat aagtgtaaag ccacgaaaac ggttgctgat tgcttaattt
     8161 gcggcttcct gacgatgaaa acctcgccac cagaccagta agttaaccag ggcaatcgtc
     8221 gcgccagcca gacaaacgcc agcccaaccg ccatgttgcc aggctgaggc tgaaattagc
     8281 gaaccggcgg caccgccaat aaagtagctg gtcatgtaac ctgcggtcag gcgattacgc
     8341 gcatcagggt gtattcgata aattaccgtc tggttagtga tatgcacgcc ctgtacggtg
     8401 agatccagca ccaggattcc gataatcaac gccagtacgg aagtgtgacc aaaccagatc
     8461 gccagccatg aaagtaatag cagcagcaga ccgaaagttg tggtgtggtg cgatttaccc
     8521 ttatcggcaa aaccgcccgc cggacgagcg cccaacgcgc cggcagctcc cgcaagtcca
     8581 aacagaccaa tgacaccatc gctgtagtta aaaggtggag cggcaagcaa aaaggccatt
     8641 gaggtccaga gaatgctgaa gttggcaaag gtcaggcagc caagcaatgc gcgggtgcgc
     8701 aggatcttat cgttgataaa catgctgaaa acggaaccca acaactgtgg gtagttgagg
     8761 tgggtttctg atttcatttg tggcagacca cgccataatg ctagcgccat cagagccatt
     8821 aacaccgagg caacccaaaa gacggtgcgc cagccgccga ggtttgccag taacccggca
     8881 acggttcgtg ccagcaatat ccccaacagc agaccgctca taatggtgcc aaccactttg
     8941 ccgcgtttat ccggtgaagc cagcgtcgct gccagcggaa ccagaatttg tgccacgaca
     9001 gagaataaac cggttaatgc cgtaccgagg agcatcatcg ccagcgactg actgctggcg
     9061 gtaatcaaca taccgcccgc tgccagtaag gtcatcgaga caatcaggcg gcggcgttca
     9121 aacatatcac cgaggggaac aagaaacagc agacctgctg catagcccaa ttgcgcggcg
     9181 gtaacaataa agcctgccga actggcggaa agggaaaagt tacgcgcgat ggtgtcgagt
     9241 agcggctggg cgtaatagtt actggcgacc gccagaccgg tggcgataga catcagcacg
     9301 atcagcgccg ggctaagctc atgattaggt ttagtcatta tttttgttca tcaggtgatg
     9361 attaagctgg aaatcaataa tagcggatgt tgatgaatat gggatgaaag atagcttgtt
     9421 gaaatgtgcg atgtggtttg taggcatgat aagacgcgtc agcgtcgcat caggcattgt
     9481 gtacagagcg gcggatgagg caaaaacgcc ttatccgccc gaataaaaat tacttctgcg
     9541 ctgccagcgc ctcattcacc cagccatcga actgctgctg gtgggctttg atccagccat
     9601 caacatggcc ctgaatatcg ccttctgagg ctttgccgtc atgcataatg gcgttctggg
     9661 cgttaatatc tgccactggc aactgcataa tggcaaacag tttcgctgct gccgggtttt
     9721 tctcggccca ggctttgttg gcaacgatat gcatggtgct gaccgggaag ccataattcg
     9781 caccattcgg cagtttggta tcggcgtttt tatcgcccgg cagtgcggag aacggcacct
     9841 gcaaccagac cacatctttc cctggcttca gctcattact cacccagtac ggcgtccagg
     9901 tgtagtaaaa caccggtttg ccctctttgt agcgactgat ggtgtcggcc atcatcgccg
     9961 cgtagttccc ctgattatgc gtcacggtat gggtcagttc atacgcggca agctggtggt
    10021 tgatcgcacc ttcgcagccc cagccagggt tacaaccggt taaatccgct tttccgtcgc
    10081 cgttggtatc gaacagtttg gcgatcttcg gatctttcag ttgtgcgatg ttggtgattt
    10141 tgtactggtc ggcggttttc ttatcgatca ggtaaccctg tgccgcgccg ttaacaaata
    10201 ccccttcacg ataaaatttc ttatcgccac cggcagcttc gtacatgttg tcatgcagtg
    10261 gcgtccagtt cacggcggtg aaggttgcat cgccggaagc aagcgaggtg tagccaacgt
    10321 tgtaatccac ttcgctgggt ttgttgacgg tataacctaa tttctccagc gcacgactga
    10381 ccagcagcgt ctggaaggtt tcttcagtga tggtgctctg aactggatta acagtaatgc
    10441 ctttgcccgg cagatcggca gcaaaagttt gtgtagagat aagcgtggca aacgctgtcg
    10501 caaaaagtac gctatgtcgc attgttattc ctttttttgg cagggtgata atgcccggcg
    10561 tcaccgggca agtgcagagt tacttaatga atgggcgggt cagcagacca acagggccag
    10621 tggtgtacca gcgacggttg ccgcgactgc gtgagtcgcg cccaacggcc tgcgtcaggc
    10681 gatcgaggat aatggcgagg atcacaatcc cgacgccgcc aacggtggca agccccatat
    10741 ccagacgacc gataccgcga agtaccatct gacccagccc gccgacggca atcatcgagg
    10801 caatgaccac catagaaagg gccagcatca gcgtctggtt aacgcccgcc ataatggtcg
    10861 gcatcgccag cggtaactga actttgaaca gcatctggcg cgggctggca ccgaatgagc
    10921 gcgaggcttc aatcagatcc gccggaacct ggttaatccc cagaatggtc agacggataa
    10981 tcggcggcag agcaaagatg atcgtcacca ccacgcccgg cacgttaccg ataccaaata
    11041 gcatgacgat tggcaccaga taaacaaacg ctggcgtggt ctgcatggca tcaagcagtg
    11101 gacgaataat tttcgccgct cgcggacttc tcgccagcca gatccccagc ggcaaaccaa
    11161 tgacgataca gaacagcagg gcggttaaca ccagcgccag ggtaaccatg gcctgcgacc
    11221 aggcaccgat tgcgccgatg gcaatcagcg aaaccagcgt cgccacgccc attccgaccc
    11281 cggaaatctg ccaggcgatg agagcgaaaa cgataatcgc caccggtgcg ggcataccca
    11341 gcagcaattg ctggaaaccg ttgagaatgt aatcaaccgg aacgcgcacg ccctggaaga
    11401 cgggacggaa atgggtaaca acccagtcga tcccttcagt gacccaactg tcgagcggga
    11461 tcagcgtttt atggaacgga tcgagaatat taaaatgctc gacgtttggc gcaggcgtac
    11521 tggtcagcca gtcagcacca ccgccgtcag tcggtgcagt cgccggtgta ccccaggcgt
    11581 ctgcggattg cacggcactg tccgccgctg gcgtggtatc ccacggatta ttttgatcag
    11641 ccattattta ccccctcacg atctaaagcg cgcagcagca ttcctttcga aatgatgccg
    11701 acatactgtt ggtcctcgtc gaccacgggc accgcacagg gtgcctgtcc gacatgagag
    11761 agcaactcgc taagaggcgt ttgtgcatcg actgctaacg gcgcatcaat cagcgccgca
    11821 tcaagacctt gctgctgcgt taacgcggtt ttaagcgaat cgatggaaac tgcgccgaca
    11881 aacttattac cgcgttcgat aacgtagcca tattcgcgat cttcatcctg caataatttc
    11941 agtgccgaac gtgggccgaa gccaggggtt ttacgaatta agccattcgg tgtccggcgg
    12001 gcaatatctt tcgcactgaa tacctgacta atatcaacgc cacggaagaa ggtacggaca
    12061 taatcattcg ccggattatt gagaatttca tccggtgtgc cgacctgtac cacttcacca
    12121 ttttgcataa tggcaattcg gtcgccaata cgcatagcct catcgagatc gtgggaaata
    12181 aagacaatgg tgcgctgatg tttcgcctgt aattttacca gctcatcctg catctcggtg
    12241 cgaattaatg gatcgagcgc cgagaaggct tcgtccatta ataatatatc cggattaatc
    12301 gctaacgcgc gggctaatcc cacacgttga cgcatcccgc cagagagttc atccgggtag
    12361 ctgtgggcat aattttccag cccgacctga cgcagtgcat caagggcttt ttcccggcgt
    12421 tcttcggcat taattccggc caattccata ccgaacgcag tattgtccag cacggtcata
    12481 tgcggcatta aggcaaagga ctggaagacc atcgcaatct tttttctgcg cacctcacgg
    12541 agttcggcat cggatatttt ggcaatatcc acaccatcaa tcagcacttg cccgcgggtg
    12601 ggttcaatca ggcgattgag aaggcgtacc attgtggatt tacccgagcc ggataatccc
    12661 atgatgacaa atatctcgcc ttcttcaatg gccagactgg cgtcttttac gccaagcgat
    12721 agcccagttt tttccagaat ttgttctttc gaaagtcctt gttcgatata tttgaacgct
    12781 cgctgtggat gctcgccaaa tattttataa agatttttaa tttctaattt aattgccatg
    12841 caatagaaag attcctttat ttgtttatgt cgatatatta cccaatggaa atagtcactt
    12901 ttttctaccc taacatactg agaatctgag gcaacccctg agggcaaatg tggcatgaat
    12961 attgatgcga gcaaatcgcc ggaatttccc gtgatataag ggctgagagc aaatcgagaa
    13021 aagttgatat tttttgtcat agatagcgaa aaaacgcctg aatattgtag tttcaggcga
    13081 ataatgcaac agagtatcag tgtagatcac tacagaatat ttgcgtgata aaaagctatt
    13141 tggcgggaat tatttccctg ctgcgggtag tgatattttt gaaaataaca ccctcagaaa
    13201 ttccagtctt catcttctgt ttcaaccgct ttccccatca cataagagga gcctgaaccg
    13261 gaaaagaaat cgtgattttc atcggcattc ggcgaaagcg cggcgaggat tgccggattc
    13321 acttccgcca tttctgcggg aaataacggt tcgtagccca gattcatcaa agccttattg
    13381 gcgttgtaac agagaaacgc tttcacatcg tcagcccacg gggtttcggc gtacagctca
    13441 tcggtgtatt gcaactcgtt gtcgtagagt tccagcaaca aatcgaaggc gaaactcttc
    13501 aactcttcac gttgtcccag agatatcttt tccatgtttt tctgatattt atagcctatg
    13561 tagtaaccgt ggactgcttc atcgcggata atcagacgga tcagatctgc ggtattggtc
    13621 agctttccgc ggctggaaaa atacatcggc agccagaaac cggaatagaa caaaaaagat
    13681 tcaagaaaca cactggcgat tttctttttc agcggatcat caccgcgata atgttgctga
    13741 ataatctgag cttttcgctg caacggtgcg ttttcttcac tccaggcgta ggcggcatcg
    13801 acatctttgg tctgacacag cgtcgagaaa atcgaactgt aagagcgggc atgaaccgct
    13861 tccataaagc tgatattcga taatactgcc tcttcatgag gcgtgagcgc atcgggcatc
    13921 agcgaaggcg cgccgataac attttgcagc gtgtcgagca gcgtcaggcc agtaaaaacg
    13981 cgcattgtca gttgttgttc tacgacactt aatgtctgcc atgcaggtat atcgttcgac
    14041 agtggcacct tttctggtag ccagaaattg ctggtcaggc gattccacac ctccagatct
    14101 ttatcgtcag atatcttgtt ccagttgatg gcgctgatac gtgagagttt catagatatt
    14161 ccttaaagtg cacaggagac gcagccttca atttcagtgc cttccagcgc catctgacgc
    14221 aggcgaatgt aatagagcgt tttgatacct ttgcgccagg cgtaaatctg cgctttgttg
    14281 atatcgcgag tggtggcggt atcggggaaa aacagcgtca gcgacaaacc ctgatcgaca
    14341 tggcgagtcg cttccgcgta ggtgtcgatg atcttttctg cgccgatttc gtaagcatcc
    14401 tgatacagcg ccagattctc gttagtcata aacggggcag ggtagtaaac gcgtcctgtt
    14461 ttgccctctt tgcgtatctc tactttcgcc acaatcggat gaatactcga cgtagcatgg
    14521 ttgatataag agatagaacc ggttggcggc accgcctgga gattctggtt gtatatgccg
    14581 tagcgcatca cgtcgtcgcg cagttgcgcc cacatctcac gggtaggtaa cgtaataccg
    14641 ctgcgggcaa acagttcgcc aactttcgcc gttttcggct gccagttacc ttgcagatat
    14701 tggctgaaat attcaccact ggcatagcgt gactgtttga acccggcgaa ggtttcaccg
    14761 cgttcgcgcg ccagcaacat cgaggtacgc agtgcgtgcc aggtgatggt atagaaatag
    14821 agattggtga aatccagggc ttccggcgaa ccataagcga tgccttccct cgccagataa
    14881 ccatgtaaat tcatctgccc cagtccgatg gcgtgcgagg cggcatttcc ggcttcgatg
    14941 gacggcacgc tgcggatatg actcatatct gacaccgccg ttaaaccgcg tacggcagtc
    15001 tctaccgtgc gggcaaagtt gggggaatcc atggtgtggg caatattcaa cgaacctaaa
    15061 ttgcaggaaa tatcatggcc tgtacgggca tagtcgagat tctcgtcata ctctgaggcg
    15121 ctgttaacct gcaaaatttc tgagcagaga ttactcatat ttatgcgccc ggcgataggg
    15181 ttagcacggt ttaccgtgtc ttcatacatg atgtagggat agccggactc aaactggatt
    15241 tctgccagtc gctggaagaa atcacgggcg ttgaggtatt ttttgcggat gcgttcatcg
    15301 gcaaccagtt cgtcatagtg ttcgctaatg gcgatatcgg caaacggctt gccataaact
    15361 cgctcaacgt catagggcga aaacagcgcc atctgcgcat tctcttttgc cagatggaaa
    15421 gtgatatccg ggatcaccac gccaagcgac agtgttttaa tgcggatttt ttcgtcggca
    15481 ttttcccgtt tcgtgtcgag aaaacgcaga atatcgggat gatgagcatg taaatagact
    15541 gcaccagccc cctgacgcgc gccgagttgg ttgggatagg aaaatgcgtc ttccagcatt
    15601 ttcatcaccg gaattacgcc agaagattga ttttcaatac gtttaattgg cgcgcccgct
    15661 tcccgcagat tcgacagcaa aaatgccacg ccgccgccgc gtttcgacag ttgcagtgcg
    15721 gaatttaccg cccggccaat cgactccata ttgtcttcaa tacgcagcag aaaacaggaa
    15781 accagttcgc cgcgctgctg cttaccgcag ttgaggaatg ttggcgtggc tggctgaaag
    15841 cgtcctgaca gcatttcatc ggtcagttgc aacgccagcg tcgcatcgcc ttgtgccagc
    15901 gtcagcgcca ccatcgtgac tcgatcggca aaatcttcca gataacgttt accgtcgaat
    15961 gtcttcaacg tatagctggt gtaaaacttc catgccccga ggaatgtctg gaaacgaaaa
    16021 ccgctggtgt gtgcgtgggc aaacagcgta atgacaaagt cgcgagagta gcgattaaga
    16081 acgctttcat catagtaacc ttcgttgacc agccagttca ggcgctgctg ctggctactg
    16141 aaggtcacac tgttcggacg cacatgcgtc gcaataaagg cgtcaacggc ctggcggtct
    16201 ttatcgaact gaatgcgacc tgcgctatcg tagaggttaa gcatcgcatt cagcgcgtgg
    16261 taatccatcg tttcctgcgt caggcattct gcggttgtcg ttgccaaaat tcggttactc
    16321 ctttacgaac gttttcgata tcgctttgcg tacccatgag ttcaaaacgg tacagccacg
    16381 gcacgccgca tttccgggca atcacatctc cggcgcggcc atacgcctca ccaaagttgc
    16441 gattaccaga agcaataacg ccgcgaagca acgcccggtt gtgctcgtcg tttaaaaagc
    16501 gaattacctg tcgtggcacc gcgccagccg taccgccgcc gccgtaagag ggcacgatca
    16561 ggatgtaagg ctcgtctacc tgaatccgtt cccgctcatt gagcgggatg cgcaccgcgg
    16621 gcagacctaa acgttcgata aaacgctgcg tgttttcgga gctgctggag aagtagacga
    16681 gctggctcat gcactggccg cgtgtggcgc tggatgcaga cggttaatca tgtccggacg
    16741 gaaaccagac cagctaagat cgccagcaat cactaccggc aactgacgaa agccctgagc
    16801 acgcaacgct tccgctgctt caggaacgcg atcgacatta atcatttcaa aatcaaagcc
    16861 ccggttttcc atcgcccgtt tggtggcgtg gcactgaacg caatcgttac gagtgtaaat
    16921 agtaatgcgc atggttcgta tttccgttta aaatgaagat acggcgcgat gatacgcgtc
    16981 gggttgtctc tctgttgata cagagatact agatgtagtt gaaaaaagat tcaaccacac
    17041 aatatatagc aaatagtggt cgaaattacc ctggatatga gcgtgacggg gtagggggat
    17101 ttttgtgatt caccaggcaa aaagaaaccc cgaagccagg cttcggggtc aaagacgcgt
    17161 atttattatc atttttgcac tacgatttgc gcatgcttaa cagtgcgccg ataaaaatac
    17221 ctaccgcagc tgctgtaccc acgctacacc agggtctttc acgaacaaaa gaatccgcgc
    17281 agccaacggc atcgcgtgcg gcttgctgga cgcgagtacg accatgcatt cgcgccctgg
    17341 tttctttcag caatgcctga gctttactgc gtgcggcttc agcttcccct ttggcgtcgc
    17401 tgccccagga cttcaataca gattcgaggc tgtcagctaa ttgattgaca tcattctgga
    17461 tatcctgcac gccatcatca acgtcgttgc ggttcggtcg gttaaacata tgatccccca
    17521 ttagaattcg atgtaatttt cagtgtagac cataattttc ttaactgagg ttcatcgcgg
    17581 ctttatggac ttttctgaaa gcgttgtgaa tgcgcaatca ggtaaggtag gcccgcggtt
    17641 gataataacg aggatcaaat atgtatttac gaccagacga ggtggcgcgc gtacttgaaa
    17701 aagtcggttt tactgtcgat gtggtaaccc agaaagcgta tggttaccgc cgtggggaaa
    17761 attatgtcta tgttaatcgc gaagcgcgaa tgggacgtac cgctctggta attcatccaa
    17821 cattaaaaga acgcagttca acactggctg aacccgcttc cgatattaaa acttgcgatc
    17881 attaccagca atttccgctc tatttagcag gcgagcgaca cgagcattac ggtatcccgc
    17941 atggctttag ttcgcgtgtt gcgcttgaac gttatttgaa tggtttattt
//
LOCUS       DAZGLJ010000088        17889 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000088 DAZGLJ010000000
VERSION     DAZGLJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.088"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(32..577)
                     /gene="syd"
                     /locus_tag="ACHK49_002566"
     CDS             complement(32..577)
                     /gene="syd"
                     /locus_tag="ACHK49_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO9263195.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            645..1493
                     /gene="queF"
                     /locus_tag="ACHK49_002567"
     CDS             645..1493
                     /gene="queF"
                     /locus_tag="ACHK49_002567"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO9263196.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDANLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            1605..2969
                     /gene="ppnN"
                     /locus_tag="ACHK49_002568"
     CDS             1605..2969
                     /gene="ppnN"
                     /locus_tag="ACHK49_002568"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO9263197.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            3526..4815
                     /gene="sdaC"
                     /locus_tag="ACHK49_002569"
     CDS             3526..4815
                     /gene="sdaC"
                     /locus_tag="ACHK49_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO9263198.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            4873..6240
                     /gene="sdaB"
                     /locus_tag="ACHK49_002570"
     CDS             4873..6240
                     /gene="sdaB"
                     /locus_tag="ACHK49_002570"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO9263199.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            6352..7107
                     /gene="xni"
                     /locus_tag="ACHK49_002571"
     CDS             6352..7107
                     /gene="xni"
                     /locus_tag="ACHK49_002571"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO9263200.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(7162..8310)
                     /gene="fucO"
                     /locus_tag="ACHK49_002572"
     CDS             complement(7162..8310)
                     /gene="fucO"
                     /locus_tag="ACHK49_002572"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO9263201.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(8338..8985)
                     /gene="fucA"
                     /locus_tag="ACHK49_002573"
     CDS             complement(8338..8985)
                     /gene="fucA"
                     /locus_tag="ACHK49_002573"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO9263202.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            9532..10848
                     /gene="fucP"
                     /locus_tag="ACHK49_002574"
     CDS             9532..10848
                     /gene="fucP"
                     /locus_tag="ACHK49_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO9263203.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            10881..12656
                     /gene="fucI"
                     /locus_tag="ACHK49_002575"
     CDS             10881..12656
                     /gene="fucI"
                     /locus_tag="ACHK49_002575"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO9263204.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQRRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            12765..14183
                     /gene="fucK"
                     /locus_tag="ACHK49_002576"
     CDS             12765..14183
                     /gene="fucK"
                     /locus_tag="ACHK49_002576"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO9263205.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            14185..14607
                     /gene="fucU"
                     /locus_tag="ACHK49_002577"
     CDS             14185..14607
                     /gene="fucU"
                     /locus_tag="ACHK49_002577"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO9263206.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            14665..15396
                     /gene="fucR"
                     /locus_tag="ACHK49_002578"
     CDS             14665..15396
                     /gene="fucR"
                     /locus_tag="ACHK49_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO9263207.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(15440..16540)
                     /gene="rlmM"
                     /locus_tag="ACHK49_002579"
     CDS             complement(15440..16540)
                     /gene="rlmM"
                     /locus_tag="ACHK49_002579"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO9263208.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(16533..16928)
                     /gene="ygdD"
                     /locus_tag="ACHK49_002580"
     CDS             complement(16533..16928)
                     /gene="ygdD"
                     /locus_tag="ACHK49_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO9263209.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(16947..17864)
                     /gene="gcvA"
                     /locus_tag="ACHK49_002581"
     CDS             complement(16947..17864)
                     /gene="gcvA"
                     /locus_tag="ACHK49_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO9263210.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
BASE COUNT         4374 a         4652 c         4383 g         4480 t
ORIGIN      
        1 agatctctca caaccagatg gggtatttgg attacagaag aggcttaagt tgattaagga
       61 attccgcaag atttgaggct aaatgcgtcc gtttacgtgt tcccagcgtc tctttgcaca
      121 cttcaccgga gagattacac accgagatga cctctaactc ttcttccagc gtcgcgataa
      181 atagcgtggg cggcagtttt agccttttct gagtcaccag atgaccaatc agattttcct
      241 gcacccgacg gaagtcgtct tcactccagg tttgcagcaa tgtcagtttg atatcgccaa
      301 actgcgcgtg catatccccg gcaaactgag tcgtataaaa ggtatgaatt gtgggttgta
      361 tcacaatatc aaaagcgcgt tcaaccgcgt ttacattttg ttccccggta aacggctggg
      421 gttgccagta tacggcatct tcggtagttg aaataatgca cggtgaagga acaccgtaca
      481 gttcctcact taacggccag cttttatgct cttcatgcca cgcatcgcag tagcgtgcag
      541 taaaatcttt cagggcttgt gcggtcaaat cgtccactaa tttctctctt cacttaaacc
      601 agatacactt gtcgtttagt ttatctggtt tatgacggtg aaacatgtct tcttatgcaa
      661 accatcaggc acttgcgggc ctgactcttg gaaaatcaac cgattaccgg gatacctatg
      721 acgccaacct actgcaaggc gttccacgca gcctgaatcg cgacccgctg ggtctgaaag
      781 cggataacct gccttttcac ggtacggata tctggacgct gtatgaactt tcctggctga
      841 atgcgaaagg tttgccgcag gttgccgtcg gtcacgttga acttgattac accagcgcaa
      901 atctgattga gtcgaagagt tttaagctct atctcaacag ttttaaccag acgcgtttta
      961 ataactggga tgaggtgcgc cagacgctgg agcgcgactt aagcacttgc gcccagggta
     1021 aggttagcgt ggcgttatat cgtcttgatg aactggaagg ccagccgata ggtcatttca
     1081 atggcacctg cattgatgac caggatatca ctatcgataa ctatgaattc actactgact
     1141 atctggagaa tgccaccagc ggtgaaaaag tagtggaaga gacgcttgtc agccacctgc
     1201 tgaaatcaaa ctgcctgatc acccatcaac cagattgggg ttcgatccaa attcagtatc
     1261 gtggacgcca aattgacaga gaaaaactgc tgcgttacct ggtctcattc cgtcatcaca
     1321 acgagttcca cgaacagtgc gtggaacgca tctttaatga cctgttacgc ttctgccagc
     1381 cagagaaatt aagcgtttac gcacgttata cccgtcgtgg cggtctggac attaacccgt
     1441 ggcgcagtaa tagcgatttt gtcccatcga ccacaagact ggttcggcaa taaatttttt
     1501 ctcaattttg cgtgctggat tcacgcagaa ggttgtgaaa ggtcatcagg cagggctatt
     1561 gtaatcaaag ggaatgacga tattcgtccc ataaggagtt tttcttgatt acacatatta
     1621 gcccgcttgg ctccatggat atgttgtcgc agctggaagt ggatatgctt aaacgcaccg
     1681 ccagcagcga cctctatcaa ctgtttcgca actgttcact tgccgtactg aactccggta
     1741 gtttgaccga taacagcaaa gaattgctgt ctcgttttga aaatttcgat attaacgtcc
     1801 tgcgccgtga acgcggcgta aagctggaac tgattaatcc cccggaagag gcttttgtcg
     1861 atgggcgaat tattcgcgct ttgcaggcca acttgttcgc ggttctgcga gacattctct
     1921 tcgtttacgg gcaaatccat aataccgttc gttttcccaa cctgaatctc gacaactccg
     1981 tccacatcac taacctggtc ttttccatct tgcgtaacgc tcgcgcgctg catgtgggtg
     2041 aagcgccaaa tatggtggtc tgctggggcg gtcactcaat taacgaaaac gagtatttgt
     2101 atgcccgtcg cgtcggaaac cagctgggcc tgcgtgagct gaatatctgc accggctgtg
     2161 gtccgggagc gatggaagcg ccgatgaaag gtgctgcggt cggacacgcg cagcagcgtt
     2221 acaaagacag tcgttttatt ggtatgaccg agccgtcgat tatcgccgct gaaccgccta
     2281 acccgctggt caacgaattg atcatcatgc cagatatcga aaaacgtctg gaagcgtttg
     2341 tccgtatcgc tcacggtatc attatcttcc ctggcggtgt gggtacggca gaagagttgc
     2401 tgtatttgct gggaatttta atgaacccgg ccaacaaaga tcaggtttta ccattgatcc
     2461 tcaccggccc gaaagagagc gccgactact tccgcgtact ggacgagttt gtcgtacata
     2521 cgctgggcga aaacgcgcgc cgccattacc gcatcatcat tgatgacgcc gctgaagtcg
     2581 ctcgtcagat gaaaaaatcg atgccgctgg tgaaagaaaa tcgccgcgac actggtgacg
     2641 cttacagctt taactggtca atgcgcattg caccagattt gcaaatgccg tttgagccgt
     2701 ctcacgagaa tatggctaat ctgaagcttt acccggatca acctgttgaa gtgctggctg
     2761 ccgacctgcg ccgtgcgttc tccggtattg tggcgggtaa cgtaaaagaa gtcggtattc
     2821 gcgccattga agagtttggt ccttacaaaa tcaacggcga taaagagatt atgcgtcgta
     2881 tggacgacct gctacagggt tttgttgccc agcatcgtat gaagttgcca ggctcagcct
     2941 acatcccttg ctacgaaatc tgcacgtaat ctcccctccc ccggtgacgt ttgttgccgg
     3001 gttttctcgt ttttggtcac ttactcatca actcatttca tttgttatat gaatgtttct
     3061 taccaccctc acgcgacaaa tatcatcaca gttaatatgt catacaattt atattgcaac
     3121 gcaaacgttt ccctattttc ataaaccgta ttttatcgct atgaaaaaga aattatcgcc
     3181 atgattaact aaaagtattg attttttcag ttcaacctac atatattgcg cgccccggaa
     3241 gaagtcagat gtcgtttaat gggcaaatat tgcccttaaa ttctctttta cttttgattt
     3301 acagagtaaa gcgttgggat aatctatctt ccaagtagat tattgtattt gggatcaaga
     3361 tcactgatag atacataact tgtgtgtatc tttccgccct caaattatta cggcggtaaa
     3421 tgattaagcc atcgccgata gacagatttc atttttacgg tcaggcacct tcccgggctg
     3481 aactggctaa aagctgaatt atttgcattc ctccaggaga aatagatgga aacgactcaa
     3541 accagcacga ttgcgtcgaa agactctcgt agtgcctggc gcaagacaga caccatgtgg
     3601 atgctgggcc tttacggcac ggcaatcggc gcgggcgtgc tgttcctgcc aatcaacgcc
     3661 ggtgttggtg gtatgatccc gctgatcatc atggctatcc ttgcgttccc gatgacgttt
     3721 tttgctcacc gcggcctgac tcgcttcgta ctgtctggta aaaacccggg cgaagacatc
     3781 accgaggttg tagaagaaca ctttggtatt ggcgcaggta aactgattac cctgctctac
     3841 ttcttcgcta tctacccgat cctgctggtt tatagcgtgg caatcaccaa tacagttgaa
     3901 agcttcatgt ctcaccagct gggtatgacg ccaccgccgc gtgcgattct gtcgctgatc
     3961 ctgatcgtgg gtatgatgac catcgttcgc tttggtgagc agatgatcgt taaagcgatg
     4021 agtattctgg tattcccgtt tgttggcgta ctgatgctgc tggctctgta cctgatcccg
     4081 cagtggaacg gcgcagcact ggaaacgctg tctctggaca ctgcatctgc aaccggaaac
     4141 ggtctgtgga tgaccctgtg gctggcaatt ccggtaatgg tgttctcgtt caaccactct
     4201 ccgatcatct cttctttcgc cgttgcgaag cgtgaagagt acggcgatat ggcagaacag
     4261 aaatgctcga agatcctggc attcgcacac atcatgatgg tgctgaccgt aatgttcttc
     4321 gtcttcagct gcgtactgag cctgactccg gcagacctgg ctgcggctaa agagcagaac
     4381 atctcgattc tgtcttacct agctaaccac tttaacgcac cggttatcgc gtggatggct
     4441 ccgattatcg cgattatcgc tatcaccaaa tccttcctcg gccactacct gggcgcacgt
     4501 gaaggcttca acggtatggt gattaaatct ctgcgtggta aaggtaagtc tatcgaaatc
     4561 aacaagctga accgtatcac tgcgctgttc atgctggtaa cgacctggat tgttgccacc
     4621 ctgaacccga gcatcctggg tatgattgaa accctgggcg gcccaatcat cgcgatgatc
     4681 ctgttcctga tgccgatgta cgcaattcag aaagtaccgg caatgcgtaa gtacagcggt
     4741 cacatcagca acgtattcgt tgtcgtgatg ggtctgattg caatctccgc aatcttctac
     4801 tctctgttca gctaagtcct ttcgcgccgc tttcgggcgg cgcttcctcc gttttaacgc
     4861 gatgtatttc ctatgattag cgtattcgat attttcaaaa tcggcattgg cccttccagt
     4921 tctcataccg ttggaccaat gaaagcgggt aaacaattta ccgacgatct gattgcccgt
     4981 aacctgctta aagacgtgac ccgcgtggtg gttgacgtgt acggctcgct ctctctgact
     5041 ggtaaaggcc accacactga tatcgccatt attatgggcc tggcgggtaa cctgccggat
     5101 accgtggata tcgattccat ccctggtttt attcaggatg tgaatactca tggtcgcctg
     5161 atgctggcaa acggtcagca tgaagtggag ttcccggttg atcagtgcat gaacttccat
     5221 gcagacaacc tttctctgca tgaaaacggt atgcgcatta ccgcgctggc gggcgataaa
     5281 gtcgtttaca gccagactta ctactctatt ggcggtggct ttatcgttga tgaagagcat
     5341 tttggccagc agaatagcgc accggttgaa gttccttatc cgtacagttc agcagccgat
     5401 ctgcaaaaac attgtcagga aaccgggctg tcactctctg gcctgatgat gaaaaatgag
     5461 ctggcgctgc acagcaaaga agagctggaa cagcacctgg cgaacgtatg ggaagtcatg
     5521 cgtggcggta ttgagcgcgg tatttccacc gaaggcgtgt tgcctggcaa actgcgcgtt
     5581 ccacgccgtg ctgcggcact acgccggatg ctggtcagcc aggataaaac caccactgac
     5641 ccgatggcgg ttgttgactg gatcaacatg tttgcattgg cagtgaacga agagaacgct
     5701 gctggcggac gcgtggtgac tgcgccgact aacggtgcgt gcgggattat cccggcagtg
     5761 ctggcgtact acgacaagtt tatccgcgaa gtgaacgcta actcactggc tcgttacctg
     5821 ctggtagcca gtgccattgg ttctctttat aagatgaacg cgtcaatttc tggtgctgaa
     5881 gtcggctgcc agggtgaagt tggtgtggcg tgctcaatgg cggcggctgg tctggcagaa
     5941 ctgttaggtg caagcccggc gcaggtgtgc atcgcggcgg aaatcgccat ggagcacaac
     6001 ctcggtctga cgtgtgaccc ggtcgccgga caggtccagg tgccatgcat cgagcgtaac
     6061 gccattgcgg cagtaaaagc ggtgaacgcc gcacgtatgg cgctacgccg taccagcgag
     6121 ccgcgcgtct gcctcgataa agttatcgaa accatgtacg aaacaggtaa agatatgaac
     6181 gccaagtacc gcgaaacctc tcgcggcggc ctggcaatga agatcgttgc ctgcgattaa
     6241 tcgctctcca gaggcctcgt tttgcgaggc ctcttcccga tttctcatcc agccgtagcc
     6301 tgttccggca tcgaatgtta cccttatcgc ctgatcttta agggggttat cgtggctgtt
     6361 catttgctta ttgtcgatgc actgaatctt attcgtcgca ttcatgccgt tcagggatcg
     6421 ccctgtgtcg aaacctgcca gcatgcgctc gatcagctca ttatgcacag ccagccaacc
     6481 cacgcggtcg ccgtttttga tgatgaaaac cgcagtagcg gctggcgtca tcagcgttta
     6541 ccagattaca aagcgggtcg accgccaatg ccggaagagt tgcacgacga gatgcctgca
     6601 ttacgcgccg cctttgagca acgcggcgtc ccgtgctggt caaccagcgg caacgaagcc
     6661 gatgacttag ccgccacgct ggcggtcaaa gtgacacagg ccgggcatca ggcaacgatt
     6721 gtttcgacag ataaaggcta ctgtcagtta ctttcaccga cattacgtat tcgtgattac
     6781 ttccagaaac gttggctgga tgcgccattt atcgataaag aatttggcgt tcaaccgcag
     6841 cagttgcccg attactgggg acttgcgggg atcagcagtt caaaggtacc gggtgttgcg
     6901 ggaatcggac caaaaagcgc cacgcagctg ctggtcgagt ttcagagtct ggaagggata
     6961 tatgagaatc tggatgcggt tgccgaaaag tggcgcaaaa aattagaaac ccataaagag
     7021 atggcgtttc tgtgccgcga tattgcccgc ttacaaaccg atttgcatat cgacggcaat
     7081 ttacagcaat tgcggttggt acggtaacgg cgagccggat acgccgcaaa cgtcgtatcc
     7141 ggcattatca catcagcgca tttaccaggc ggtatggtaa agctctacaa tatcctcaag
     7201 cgttgcttca cgcgggttgc caccggtaca aacatcatcc agtgccgcct gcgccagtgc
     7261 cggaatgtct tccttgcgta ccccaacatc acgcaaatgt ggcggaatac cgacatcacg
     7321 gttgagagca aacaccgctt caacagcggc attacgcgcc tcttccaggc tcataccttc
     7381 cactttcacg cccataacgc gcgcgatatc gcggtacttc tcaccggtaa agtcagcgtt
     7441 atagcgcatg acatgcggta gcaggatggc gtttgcaaca ccgtgtggag tgttataaaa
     7501 cgcgcccagt ggatgcgcca taccatgcac caaccctaac ccaacattcg agaagcccat
     7561 acccgcaaca tactgcccga gcgccatttc ttctccggca tccttatcac cagcaaccga
     7621 tcctcgcagc gccccagcaa tgatttcaat cgctttaatg tgcagtgcat cggttagcgc
     7681 ccacgcgcca cgggtaatat acccctcaat agcatgagtg agcgcatcga cacccgtcgc
     7741 agctttcagc gctggaggca taccatccat catgtcagcg tcaataaacg ccacctgcgg
     7801 gatatcatgc ggatcaacgc aaacaaactt gcgccgtttt tcttcgtcag tgatcacgta
     7861 gttaatggtc acttctgccg cagtgcctgc tgtggtgggg attgccagaa tcggtacact
     7921 gggtttattg gtcggggaaa gtccttccag gctacgcaca tcggcaaact ccgggttgtt
     7981 gctgataatg ccaatcgctt tacaagtatc ctgtggagaa ccaccaccaa tagcgatcag
     8041 gtaatccgcg ccgctattct ggaatacacc gagcccttct ttgacgacag taattgttgg
     8101 gttgggcact acgccgtcgt aaatcgccca tgccagccct gcagcatcca tcttatcggt
     8161 cactttcgcc accacgccgc attgcaccag cgttttatcg gtgacgatca gcgccttctg
     8221 ataaccacgg cgtttcacct catcggttaa agccccaaca gcaccccgac caaaccatgc
     8281 cgtttcgttc agaatcattc tgttagccat catccttctc cttgttgctt tacgaaatta
     8341 ctcttcaatt cgtaacccat aggttttgaa tttctccagc actacggcaa tctcttcatc
     8401 gctcagcact ggcaccgggt ccgtaatcgc cagggtcgtc aggtaaagtt gcgccagcac
     8461 ttcaacttca tgcgccagcc ataacgcttt ttccagattc acctcacaag cgataagccc
     8521 atgatgttgt aacaaagttg ccttacgatt tttgagagcc tgcgcaacat gttcagaaag
     8581 ttcacgtgtt ccaaaggtcg cataaggcgc gcaaggaata gaattaccgc cagccgccgc
     8641 aatcatgtag tgaatagcgg ggatcgatcg gttaagaatg gaaactgccg tgcaatgaac
     8701 ggcatgattg tgaacaaccg cgttggcatc cggtctgctt tgataggccg ccatatggaa
     8761 acgccattcg cttgagggga gctttccttc ctcatgttta ccgttgccat caataaagac
     8821 aatatgcgac tccgtcagtt tttcatatgg aatgcctgta ggcgtaatca gcatcccatc
     8881 ctgataacgt acactgacgt tccccgctgt cccctggttc agtcccaggc gggtcatttc
     8941 caggcaagtg tcaataatct gacgagcaag tttatttcgt tccattagct acctctctct
     9001 gattcaaaac agggcaataa tgttgttcct ttcacactat tgaattagcc gtttaattac
     9061 ccaccatctt cttcctgatt aaccagaaag aaattcacaa gcttatattt tgtgacctgg
     9121 ttcaactaat cacagtaaat aactgcaagt tctcttttta taaccccatt aaaaatgacc
     9181 gctcttaaaa atatttatca aaacggtcat ttttctattc actcaagcca ggaatgcccg
     9241 ttttcggcat aaacaattaa tacggtcatc tggtttgtgt tttttatgat ttattttctg
     9301 aaacgggcat gaaatttcga ttattaaagt gatggtagtc acataaagtc accttccagc
     9361 taataagtgt gaccgccgtc atattacaga gcgtttttta tttgaaaatg aatccatgag
     9421 ttcatttcag acaggcaaat attcactgat atgaagcccg aactcgctgg ttttgcactt
     9481 ttgaaaacat aaccgattac gtgcttaagc ttctgaacct aagaggatgc tatgggaaac
     9541 acatcaatac aaacgcagag ttaccgtgcg gtagataaag atgcagggca aagcagaagt
     9601 tacattattc cattcgcgct gctgtgctca ctgttttttc tttgggcggt agccaataac
     9661 cttaacgaca ttttattacc tcaattccag caggctttta cgctgacaaa tttccaggct
     9721 ggcctgatcc aatcggcctt ttactttggt tatttcatta tcccaatccc tgctgggata
     9781 ttgatgaaaa aactcagtta taaagcaggg attattaccg gattattttt atatgccttg
     9841 ggcgctgcat tattctggcc cgctgcagaa ataatgaact acaccttatt tttagttggc
     9901 ctatttatta ttgcagccgg attaggttgt ctggaaactg ccgcaaaccc ttttgttacg
     9961 gtattagggc cggaaagcag tggtcacttc cgcttaaatc ttgcgcaaac atttaactcg
    10021 tttggcgcaa ttatcgcggt tgtctttggg caaagtctta ttttgtctaa cgtgccacat
    10081 caatcgcaag acgttctcga taaaatgtct ccagagcaat tgagtgcgta taaacacagc
    10141 ctggtattat cggtacagac accttatatg atcatcgtgg ctatcgtgtt actggtcgcc
    10201 ctgctgatca tgctgacgaa attcccggca ttgcagagtg ataatcacag tgacgccaaa
    10261 caaggatcgt tctccgcatc gctttctcgc ctggcgcgta ttcgccactg gcgctgggca
    10321 gtattagcgc aattctgcta cgttggcgca caaacggcct gctggagcta tttgattcgc
    10381 tacgctgtag aagaaattcc aggtatgact gcaggctttg ccgctaacta tttaaccgga
    10441 accatggtgt gcttctttat tggtcgtttc accggtacct ggctcatcag tcgcttcgca
    10501 ccacacaaag tcctggccgc ctacgcatta atcgctatgg cactgtgcct gatctcagcc
    10561 ttcgctggcg gtcatgtggg cttaatagcc ctgactttat gcagcgcctt tatgtcgatt
    10621 cagtacccaa caatcttctc gctgggcatt aagaatctcg gccaggacac caaatacggt
    10681 tcgtccttca tcgttatgac catcattggc ggcggtattg tcactccggt catgggtttt
    10741 gtcagtgacg cggcgggcaa catccccact gctgaactga tccccgcact ctgcttcgcg
    10801 gtcatcttta tctttgcccg tttccgctct caaacggcaa ctaactgaac atattttccg
    10861 aataaagtga ggaatctgta atgaaaaaaa tcagcttacc gaaaattggt atccgcccgg
    10921 ttattgacgg tcgtcgcatg ggtgttcgtg agtcgcttga agaacaaaca atgaatatgg
    10981 cgaaagctac ggccgcactg ctgaccgaga aactgcgcca tgcctgcgga gctgccgtcg
    11041 agtgtgtcat ttccgatacc tgtatcgcgg gtatggctga agccgctgct tgcgaagaaa
    11101 aattcagcag tcagaatgta ggcctcacca ttacggtaac gccttgctgg tgctatggca
    11161 gtgaaaccat cgacatggat ccaacccgcc cgaaagccat ttggggcttt aacggcactg
    11221 aacgtcccgg cgctgtttac ctggcagcgg ctctggcagc tcacagccag aaaggcatcc
    11281 cggcattctc catttacggt catgacgttc aggatgccga tgacacatcg attcctgccg
    11341 atgttgaaga aaaactgctg cgctttgccc gcgccggttt ggccgtcgcc agcatgaaag
    11401 gtaaaagcta tctgtcgctg ggcggcgttt cgatgggtat cgccggttcc attgttgatc
    11461 acaacttctt tgaatcctgg ctgggaatga aagtccaggc ggtggatatg accgaactgc
    11521 gtcgccgtat cgatcagaag atttacgacg aagccgaact ggaaatggca ctggcctggg
    11581 ctgataaaaa cttccgctat ggcgaagatg aaaataacaa acagtatcaa cgcaatgccg
    11641 agcaacgccg cgcagttctg cgcgaaagtt tactgatggc gatgtgtatc cgcgacatga
    11701 tgcaaggcaa cagcaaactg gccgatattg gtcgcgtgga agaatcactt ggctacaacg
    11761 ccatcgctgc gggcttccag gggcaacgtc actggaccga tcaatatccc aatggtgaca
    11821 ccgccgaagc gatccttaac agttcatttg actggaatgg cgtgcgcgaa ccctttgtcg
    11881 tggcgaccga aaacgacagt cttaacggcg tggcaatgct aatgggtcac cagctcaccg
    11941 gcaccgctca ggtatttgcc gatgtgcgta cctactggtc accagaagca attgagcgtg
    12001 taacggggca taaactcgat ggactggcag aacacggcat catccatttg atcaactccg
    12061 gttctgctgc gctggacggt tcctgtaaac aacgcgacag cgaaggtaac ccgacgatga
    12121 agccgcactg ggaaatctct cagcaagagg ctgacgcttg cctcgccgct accgaatggt
    12181 gcccggcgat ccacgaatac ttccgtggcg gcggttactc ttcccgcttc cttaccgaag
    12241 gcggcgtccc gttcaccatg acccgtgtca acatcatcaa aggcctggga ccggtactgc
    12301 aaatcgcgga aggctggagc gtggaattgc cgaaggatgt gcatgacatc ctcaacaaac
    12361 gcaccaactc aacctggcca accacctggt ttgcaccgcg cctcaccggt aaagggccgt
    12421 ttacggatgt gtactcggta atggcgaact ggggcgctaa ccatggggtt ctgaccatcg
    12481 gccacgttgg cgcagacttt atcactctcg cctccatgct gcgtatcccg gtatgtatgc
    12541 acaacgttga agagaccaaa gtgtatcgtc cttctgcctg ggctgcgcac ggcatggata
    12601 ttgaaggcca ggattaccgc gcttgccaga actacggccc gttgtacaag cgttaataca
    12661 ttactccctg atgtgatgcc cggtcgctcc ggctaccggg cctgaacaag caagagtggt
    12721 tagccggata agcaatgtta tccggctata ttgcaggagc gattatgaaa caagaagtta
    12781 tcctggtact cgactgtggc gcgaccaatg tcagggccat cgcggttaat cggcagggaa
    12841 aaattgttgc ccgcgcctca acgcctaatg ccagcgatat cgcgatggaa aacaacacct
    12901 ggcaccagtg gtctttagac gccattttgc aacgctttgc tgattgctgt cggcaaatca
    12961 atagtgaact gactgaatgc cacatccgcg gtatcgccgt caccaccttt ggtgtggatg
    13021 gcgctctggt agataagcaa ggcaatctgc tctatccgat cattagctgg aaatgtccgc
    13081 gaacagcagc ggttatggac aatattgaac agttgatctc cgcacagcgg ttgcaggcta
    13141 tttctggcgt cggagccttt agtttcaata cgttatataa gttggtgtgg ttgaaagaaa
    13201 atcatccaca actgctggaa cgcgcgcacg cctggctctt tatttcgtcg ctgattaacc
    13261 accgtttaac cggcgaattc actactgata tcacgatggc cggaaccagc cagatgctgg
    13321 atatccagca acgcgatttc agtccgcaaa ttttacaagc caccggtatt ccacgccgac
    13381 tcttccctcg tctggtggaa gcgggtgaac agattggtac gctacagaac agcgccgcag
    13441 caatgctcgg cttacccgtt ggcataccgg tgatttccgc aggtcacgat acccagttcg
    13501 ccctttttgg cgctggtgcc gaccaaaatg aacccgtgct ctcttccggt acatgggaaa
    13561 ttttaatggt ccgtagcgcc caggttgata cttcgctgtt aagtcagtac gccggttcca
    13621 cctgcgaact ggatagccag gcagggttgt ataacccagg tatgcaatgg ctggcatccg
    13681 gcgtgctgga atgggtgaga aaactgttct ggacggctga aacaccctgg caaatgttga
    13741 ttgaagaagc tcgtctgatc gcgcctggcg cggatggcgt aaaaatgcag tgtgatttat
    13801 tgtcgtgtca gaacgctggc tggcaaggag tgacgcttaa taccacgcgg gggcatttct
    13861 atcgcgcggc gctggaaggg ttaactgcgc aattacagcg caatctacag atgctggaaa
    13921 aaatcgggca ctttaaggcc tctgaattat tgttagtcgg tggaggaagt cgcaacacat
    13981 tgtggaatca gattaaagcc aatatgcttg atattccggt aaaagttctc gacgatgccg
    14041 aaacgaccgt cgcaggagct gcgctgttcg gttggtatgg cgtaggggaa ttcaacagcc
    14101 cggaagaagc ccgtgcacag attcattatc agtaccgtta tttctacccg caaactgaac
    14161 ctgaatttat agaggaagtg tgaaatgctg aaaacaattt cgccgttaat ttctcccgaa
    14221 ctattgaaag tgctggcaga gatgggacat ggagatgaaa ttattttttc cgatgctcac
    14281 tttcccgccc attcgatggg accgcaggtg atccgcgctg atggcctgtt ggtgagcgac
    14341 ttgctccagg cgattatccc gttatttgag ctggacagtt atgccccgcc gctggtgatg
    14401 atggcagcgg tagaaggtga cactctcgat cctgaagtag aacgacgtta ccgtaatgcg
    14461 ctttcactac aagccccgtg tcctgacatc atccgcatca atcgttttgc gttttatgaa
    14521 cgggcgcaaa aagcctttgc gatcgttatc acaggcgaac gagcgaagta cgggaatatt
    14581 cttttaaaaa aaggggtaac accgtaatct cataccggta cgcccgcatg acgcgggcgg
    14641 ttatcgaatg atggggtgaa aaatatgaaa gcggcacgcc agcaagcgat agtcgacctg
    14701 ctgctgaacc ataccagcct gaccacggaa gctctctctg aacagctaaa ggtcagtaaa
    14761 gaaaccattc gtcgcgatct caatgaatta cagacgcagg gtaaaattct gcgcaatcat
    14821 ggacgcgcta aatatatcca ccgtcaaaat caagacagtg gcgatccctt tcacatccgg
    14881 ctgaaaagcc attatgcgca taaagcagat atcgcgcgcg aggcgctcgc gtggattgaa
    14941 gaagggatgg tgatagcctt agacgccagt tcaacttgct ggtatctggc acgccagttg
    15001 cctgacatca acattcaggt cttcaccaat agccatccga tttgccatga actcggtaaa
    15061 cgcgaacgca ttcaactgat cagttccggc ggcacacttg agcgcaaata tggctgttac
    15121 gtcaatccct cgctgatttc ccaacttaaa tcgctggaaa tcgatctgtt tattttttct
    15181 tgtgaaggga tcgatagcag cggcgcactg tgggactcca atgccatcaa cgctgattac
    15241 aaatcgatgc tattaaaacg tgccgcgcaa tcgttgttat tgattgataa aagtaaattt
    15301 aatcgttcag gggaagcccg catcgggcat ctggatgagg taacgcacat tatttctgat
    15361 gagcgccagg ttgcaacttc tttggtaaca gcctgacgga aagggtagca ggccggagac
    15421 gacgccccgg ccttgcctgt tatcgctcgt cgcgacgacc acccaccgca gcccagatgc
    15481 ggcggacgtg caccgtcact tcttcgcgat cgtgatacaa ctgccgcgcc tgaatctgag
    15541 catttatgcc atgttcatca agctgtgcct gaatatacgc cagattgtgt gatacttctt
    15601 cgtagcgttt tttcatcggc agtttgagat tgaaaatggt ttcacggcac cagccattaa
    15661 ccagccactg cgccatcaat gccgcaactt tagccggttt ttcaaccata tcgcatacca
    15721 tccaggagat attgctgcgt gtcggacgga atttgaaacc gtcttcccgc agccacgtca
    15781 cctgcccggt atccatcaga ctttgcgcca tcgggccgtt gtcgacggaa taaacccaca
    15841 tgttgcgctt caccagttgg taggtccagc cgcctgggca agcgcccaaa tccaccgccc
    15901 acatcccgtt cgccaggcgt tcatcccact catccgcagg aataaacaca tgaaatgcct
    15961 cttccagttt gagtgtggaa cgactcggcg catctgccgg aaatttcagg cgcggaatgc
    16021 ccatatagaa cggcgaatta ttgttgctgt atgagtaacc ggtatagcag caacctggtg
    16081 caatgaagaa tacatgcaca accggacgct tcggcgtttc atagttcgcc agcaccccgg
    16141 catcgcgcaa ggcagcgcgt agcggcacgg tgaatttacg gcagaatttc agcaactctt
    16201 tgctttcgtt ggtatcggca acttcaacac gcagttcacc gcccttctct actacgccct
    16261 gtaacatgcc gacaatgggg gtaatacgat cttctggcgg caaatgctgc aggagttccc
    16321 ccaccacaaa ccactggcgg gcaaaaatta atgaactgaa cggcagctca cggattaact
    16381 tatcgccatc atcaggttga taacattcat aaatgacata acccgcattc tctttcacgc
    16441 gggcaaaacc gaaaatttcc cgctggccgg ctttatcggt aatttctgcg gcgcactctt
    16501 tttcaaagcc cggacggcac agcaatacaa ccttattcat gacttacgcc cttgcgcttt
    16561 aaacggatag caccaactaa cattaacgcc cagcccgcaa ggaagctcac gccgccaacc
    16621 ggagtgacaa acgcccacaa acgcagatgg gacagtgcca ggcaataaag gctgccgctg
    16681 aacagcaccg tgcctaacgc gaggaaaacg ctactccagt aaaaccagat actgatgcga
    16741 cgctgcattg ccaccgccag acctaagatc gccagcgtat gaaacgcctg gtattcgagg
    16801 ccggtctgga tccagcccat ctcaacggcc cccatggttt tacttaacac atgcgcgcca
    16861 aaagcgccca gagccacaaa aatgaagccg ctaatggcgg cgaaaatcag cataaaacgg
    16921 ctggtcatgg tcgtacccta cgtaaattat tgttcataac gaaagcggaa tttttcttgt
    16981 tcagcagcgg ctttcgccag gatccattgg cgaaaggcgg ctattttacc cagttctgcc
    17041 tggctgtcat gacaaaccag ataaaaagcg tttttactga ccagaacatc attaaacggg
    17101 caaacaagac gtccggcttc aatttcagat tgcgccatca cgttatttgc cagcgccact
    17161 ccctgcccgt gaatagccgc ttgcagcacc atggcgctat gactaaaaat tggcccttgc
    17221 tgaacgttga tatgatttaa ccccaactgt cgggtatatg tctgccagtc acggcgtgac
    17281 gcatcatgta ataacgtatg tttagccaga tcttccgggg tcttcaaggg tttttcgcct
    17341 gtcagcagta gcggcgaaca caccggcaat aaatattcgg cgtacagttt ttccacccgt
    17401 agccccggcc agttgccccg accataaaat atcgccacat caacatcatc cgccagctta
    17461 tcttcctgac gatcaaccgc ctggattcga acgtcaattc ccggataagc tgaattaaag
    17521 ctggaaagtc gcggaaccaa ccaatgaatg gcgaaactgg ggagtaaact gaccgtcaac
    17581 gcccccttgg cgctacgggc ctggagttta cgcgtcgctt cggttaattg cgaaaatatc
    17641 tctttgatat cgaggaaata gctttgccct tcctcggtca gcaggagtga acgattacgg
    17701 cggcggaaca gttttagccc caaaaaatcc tcaagagact tgatttgatg acttactgcg
    17761 gcctgggtca caaaaagctc ttctgctgcg cgagtgaaac ttaaatggcg tgctgcggca
    17821 tcaaaaactc gtaaggcatt tagcggtggt aatcgtttag acatggcttt taaactttga
    17881 tgttaaatg
//
LOCUS       DAZGLJ010000089        17734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000089 DAZGLJ010000000
VERSION     DAZGLJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.089"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..354
                     /locus_tag="ACHK49_002582"
     CDS             <1..354
                     /locus_tag="ACHK49_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO9263211.1"
                     /translation="NGTEIAITYVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEP
                     LSAKYKNIAGVEEKLTYTDTYAQENVTIDMEKVDFKALQGISGINVSAEDAKKGITMA
                     QMELVMKAAGFKEVK"
     gene            complement(394..864)
                     /gene="yehS"
                     /locus_tag="ACHK49_002583"
     CDS             complement(394..864)
                     /gene="yehS"
                     /locus_tag="ACHK49_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO9263212.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIFLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(911..1630)
                     /gene="btsR"
                     /locus_tag="ACHK49_002584"
                     /gene_synonym="yehT"
     CDS             complement(911..1630)
                     /gene="btsR"
                     /locus_tag="ACHK49_002584"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO9263213.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1627..3312)
                     /gene="btsS"
                     /locus_tag="ACHK49_002585"
     CDS             complement(1627..3312)
                     /gene="btsS"
                     /locus_tag="ACHK49_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO9263214.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3534..4265
                     /gene="mlrA"
                     /locus_tag="ACHK49_002586"
     CDS             3534..4265
                     /gene="mlrA"
                     /locus_tag="ACHK49_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO9263215.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            4325..4432
                     /gene="yohO"
                     /locus_tag="ACHK49_002587"
     CDS             4325..4432
                     /gene="yohO"
                     /locus_tag="ACHK49_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO9263216.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(4413..5144)
                     /gene="yehW"
                     /locus_tag="ACHK49_002588"
     CDS             complement(4413..5144)
                     /gene="yehW"
                     /locus_tag="ACHK49_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO9263217.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(5149..6075)
                     /gene="yehX"
                     /locus_tag="ACHK49_002589"
     CDS             complement(5149..6075)
                     /gene="yehX"
                     /locus_tag="ACHK49_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO9263218.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(6068..7225)
                     /gene="yehY"
                     /locus_tag="ACHK49_002590"
     CDS             complement(6068..7225)
                     /gene="yehY"
                     /locus_tag="ACHK49_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO9263219.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(7232..8149)
                     /gene="osmF"
                     /locus_tag="ACHK49_002591"
     CDS             complement(7232..8149)
                     /gene="osmF"
                     /locus_tag="ACHK49_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO9263220.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGSVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(8360..10657)
                     /gene="bglX"
                     /locus_tag="ACHK49_002592"
     CDS             complement(8360..10657)
                     /gene="bglX"
                     /locus_tag="ACHK49_002592"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO9263221.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            10853..12568
                     /gene="dld"
                     /locus_tag="ACHK49_002593"
     CDS             10853..12568
                     /gene="dld"
                     /locus_tag="ACHK49_002593"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO9263222.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(12606..13538)
                     /gene="pbpG"
                     /locus_tag="ACHK49_002594"
     CDS             complement(12606..13538)
                     /gene="pbpG"
                     /locus_tag="ACHK49_002594"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO9263223.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(13712..14299)
                     /gene="yohC"
                     /locus_tag="ACHK49_002595"
     CDS             complement(13712..14299)
                     /gene="yohC"
                     /locus_tag="ACHK49_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO9263224.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            14469..15047
                     /gene="yohD"
                     /locus_tag="ACHK49_002596"
     CDS             14469..15047
                     /gene="yohD"
                     /locus_tag="ACHK49_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO9263225.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(15177..15938)
                     /gene="yohF"
                     /locus_tag="ACHK49_002597"
     CDS             complement(15177..15938)
                     /gene="yohF"
                     /locus_tag="ACHK49_002597"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9263226.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATYEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(15991..17427)
                     /gene="mdtQ"
                     /locus_tag="ACHK49_002598"
     CDS             complement(15991..17427)
                     /gene="mdtQ"
                     /locus_tag="ACHK49_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO9263227.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            17651..17734
                     /gene="yohP"
                     /locus_tag="ACHK49_002599"
     CDS             17651..17734
                     /gene="yohP"
                     /locus_tag="ACHK49_002599"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO9263228.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         4314 a         4866 c         4415 g         4139 t
ORIGIN      
        1 aacggcactg aaattgccat tacctatgtc tacaaaggtg acaaggtgct taagcaatct
       61 tctgaaacca aaattcaatt tgcttctatt ggtgcaacca ccaaagaaga cgctgccaag
      121 acacttgagc cgttaagcgc caaatacaaa aacatcgcgg gtgttgaaga aaaattaacc
      181 tatacagata cctacgcgca ggaaaacgtg actatcgata tggaaaaagt ggattttaaa
      241 gccctgcagg gtatttcagg aatcaacgtt tctgctgaag atgccaaaaa aggtatcact
      301 atggcgcaaa tggaactggt gatgaaagcc gctggtttta aagaagtgaa ataatcggtt
      361 ggcggtcatg ctctaaacat gaccgccaat tttttagcct tttttcacat gctggcgcgc
      421 tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg cattcgcgga agtttttatg
      481 atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa acgcggaact gctgttcggt
      541 gaggatagcc agaatgtcat cggttttcag cgaaaacgcg atgcgtaatt ttttcagcac
      601 gatgttgtta ttaatgcgac gttccggctc cagtgccgga gcagactcat ccttgccgcg
      661 tttttcataa atcaggccat tgaggaatga cgacaaaaaa atgtccggac aacgctgaaa
      721 accctcttcg tcttctttac gtagccagac ggcgatctgt tccgcggtgg cttcgacatt
      781 acccagcgcc agaatacgca ccaggtcatt attattggct ttcaaaatgt agcgcacgct
      841 gcgcagaata tcgttactta gcatgaggcc ttcaggtgtt gatgaggcaa aaagccattt
      901 tagcagtctt ttacaggcca atcgcctctt ttaagctttt cagataacgg cggctgaccg
      961 gcacggttaa gccattacgc aaaatcaact cggcctggcc gttatcttcc agacgaatct
     1021 cctgtaaatg cgcgaggtta accagatact gacgatggca gcgcagtagt ggtgtacgac
     1081 tttccagggt acgtaatgtc aattcggtaa agccctcttt cccttcgtgg ctggtaacgt
     1141 agacaccgct catccgactg ctgacaaatg ccacatcttt catttgcagc aaataaatcc
     1201 gactatgccc cgtacaaggg ataaatttca gcgcctgttg attttccggt aacagcgaaa
     1261 catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt tttctccagt cgcgcttcat
     1321 caattggctt cagcagataa tcaaaggcat gttcttcaaa ggctttaata gcgtattcgt
     1381 caaacgcagt gagaaaaaca atatacgggc gatgttccgg gtcaagcatc cccaccattt
     1441 ccagaccact gatgcgcggc atctggatat cgagaaacag cacatccggg cgcagtttat
     1501 gcaccgcgcc gatcccttcc acggcgtttg aacactctcc aacgatttca atatcgctct
     1561 gctcctgcaa aaatacgcgc aggttctccc gtgctaacgg ttcatcatcg acaattaaga
     1621 ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt attcgggtgt aactatcagg
     1681 ctcacaggcg acgcttattc catagtcatc gccaaaccgt tcacgtaaac gcttatccac
     1741 cagattcatc cccagcccac tggcattggt taccggttga tacaaaccgg cattgtcttc
     1801 gatctccagc atcaaatgtt gcccctcacg tcgggcgctg attgccactc gccctgtatc
     1861 cagcagttgt gatgtcccat gtttaatggc gttttccact atcggttgca gggtaaacgc
     1921 gggcaattgc tgctgggata attcttgcgg aatagcaatg ttgacctgca accgcgactg
     1981 gaagcgcgcc ttttcaattt gcagataagc gttcacatgt tcaatttcgt cggcgagagt
     2041 aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa gtggaaagat actgcaccag
     2101 ctggctggcc tgttcgctgt cgcggcggat caccgcttta atggtgttaa gcgcattaaa
     2161 caaaaaatga ggattcacct gggcgtgaag cagtttgatt tctgactggg tgagcatcgc
     2221 tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa agcagttgcg caatcccctc
     2281 gcccagcgtg cggtttattg aactgaataa acggtttttg gcttcataca atttgatggt
     2341 gcccatcacg cgctgatttt cgccacgcaa cggaattacc agcgtcgacc ccagtttgca
     2401 ttgcggatgc aaagagcaac gataaggtac ttcgttgcca tcagcgtaga ccacttcacc
     2461 ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt ttgccgggta aatggtggtc
     2521 gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg gtaatcgcga ctgcaccaat
     2581 atccagctcc tgatacagca cctgagccac tttcatgctg ttcacttcgt taaacccctg
     2641 tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca gtggcagaaa aagcagaagt
     2701 gtatttttca aacatcgcgc gtttatcgag caatatacgc ataaacagcg ccgcgccgac
     2761 ggtattggtg accatcattg gtgcagcaat attactcacc agacgcaccg catcttcata
     2821 aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt tcagcgacga acgtgacggc
     2881 accggcggta atggggttaa agactttatc agtgcgcccg cgacggatca ggatgctgtg
     2941 taccaggccg ccgagcaatc cttcaacgat ggttgagatc atgcaactta gcgcggtcat
     3001 gccccccatc gaatatcgat gtaagccgcc ggtcagacca accagcccac cgacgaccgg
     3061 accgccgagt aagccgccca ttaccgcgcc tatcgcacgg gtattggcaa tagaatcgtc
     3121 aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg gaaaagacga tgtagcagag
     3181 aaatttatgc ggcagacgaa ccgtgacctg cattaacggt atgaataatg gcgttttact
     3241 cattaaccac gcaatgacta aaaaaacgca catctgctga agcagcagca acaccagatt
     3301 aaaatcgtac atacccgcaa accacacttc cctttaaaac gcgtaacata cattgcctgc
     3361 gtttaacttt ctttgaactc ttgcagaaaa atgagaattc gtgagtacga tcactcaaaa
     3421 tcgcctggca aaaataaaat caccctatag atgcacaaaa aacgggcaaa actacctggt
     3481 tcgcaaaact gcgtctaaag ttaaaccggg acctcgcgag caagggtgag acgatggcgc
     3541 tttacacaat tggtgaagtg gcgttgcttt gtgatattaa ccctgtcacg ttacgcgcgt
     3601 ggcagaggcg ttacgggttg ctgaaaccgc aacggacaga cggcggtcat cgactgttca
     3661 acgatgccga tattgaccgt atccgcgaga tcaaacgctg gatcgacaac ggcgtgcagg
     3721 tcagcaaagt taaaatgctg ctcagtaatg aaaatgttga tgtgcagaac ggctggcgcg
     3781 atcagcaaga aacattactg acctacctgc aaagcggcaa tctgcatagc ctgcgaacgt
     3841 ggatcaaaga gcgcggtcag gattaccccg cccagacact caccacacat ctgtttattc
     3901 ctctgcgccg acgacttcaa tgccaacaac cgactctcca ggcgctgctg gcgatcctcg
     3961 acggcgtact gatcaactac atcgccattt gtctggcttc ggcacgtaaa aaacagggta
     4021 aagatgcgct ggtggttggc tggaatattc aggataccac ccgtctgtgg ctggagggct
     4081 ggattgccag tcaacaagga tggcgcattg atgtcctcgc ccactcgctc aatcaactac
     4141 gccctgaact attcgaaggt cgtacattgc tggtgtggtg cggtgaaaat cgaacctccg
     4201 cccaacagca gcaactcacc agttggcaag aacaaggcca tgatattttc ccactcggca
     4261 tttaatgatt cgttaacaaa tgcgctttac tgtacaatcc tttcgttaac ataaggagtg
     4321 cattatgcgc atagctaaaa ttggggtcat cgccctgttc ctgtttatgg cgttcggcgg
     4381 aattggtggc gtcatgctcg caggttatac ctttattttg cgtgctggct aagcgcctgc
     4441 accagccttt caaacaggcg gtctgcgatg atcgccgcca gtgccaccag taacgccccc
     4501 tggatcacat acgcggtatt aaatccgcta agcccgatga tgatgggcgt acccagcgtg
     4561 ctggccccta ccgttgaggc gatcgtcgcc gtaccaatgt tgataatcac cgaagttcgc
     4621 acgcccgcca gaatcaccgg agccgccagc ggtagctcga ccttacgcag tcgctgacca
     4681 cgactcattc ccataccttt cgcaacttct gtcacgctgg catcgatcgc tcccagcccg
     4741 gcaagtgtcg cctgcaggac gggcagcaca ccgtaaagga tcaaggcgat aatcgctggt
     4801 tgcagaccaa agccgatcac cggaacggca atcgccagca ctgcgacagg cggaaaagtc
     4861 tgtccaacgg cggcaatagt ttccaccagt gggcgaaatt ccgcgcccca cgggcgagtg
     4921 acagcaattc cggcaccagt gccaatgatc accgcaaaca aactcgaaat tcccaccagc
     4981 cagaaatgag ccagtgccag agctgcaaaa ctttcttgct gataaacggg tcgtggcagt
     5041 tgtgggaaca aggcagcaaa cagcggctgg ctgtaaggca gccagaaaat cagtgccaca
     5101 aacaaagcaa tgagccagaa cagcggatcg cgcaacatct tcatacgctt acgcctccac
     5161 cagcagatcc tgaaaatgca gcgtaccgca aggctggccc tgcgtgttca ccaccggcag
     5221 cacctcgcaa ccccgcgcga caaacaggga gagcgcatcg cgtagcgtca tctcttctgc
     5281 cagtgcctca ccttctgccc gttcttcgcg acgcacgtaa tccgccacac tacgtaacga
     5341 aagcagacgc acacccagtt cgctacgtcc aaaaaactgg cggacaaaat cattcgccgg
     5401 acgagtcagc atcgtcagcg gattgccctg ctgcaccact tcaccgtgat ccatcaatac
     5461 cagatgttca gccagccgta gcgcctcatc aatatcatga gtgaccagca caatagtgcg
     5521 ccccagcaaa cggtgaatgc gcgtcatctc ttgttgcaac gcgccgcgcg ttaccgggtc
     5581 cagtgcgcca aaaggttcat ccatcagtaa gacttgcgga tcggcagcca gtgcgcgcgc
     5641 cactcccaca cgttgctgct gaccaccgga aagctgatgc ggataacgct cacgcaaatt
     5701 tgactccagc cccagtagcg tcattaattc gtcgatacga tcgtcaatcc gtgcccgcga
     5761 ccatttttgt aattgcggca cggtagcgat gttttgcgcc acgctccagt gggggaatag
     5821 gccaatagat tgaatggcat agcccatccg gcggcgcaac tccagtactg gcagcgagcg
     5881 aatttcttct ccggcaaagc ggatctctcc gctgtcatgt tccaccaggc ggttaatcat
     5941 tttcaaggtg gtggatttgc cggagccaga tgtgccaatc agcaccgaaa aactcccttc
     6001 ctgaaaattg agattaagat cgttaacggc tttttgtgcg ccaaacagtt tgctgacatg
     6061 gctaaattca atcattacgt ttcaccttca gcagtgcgat aagcaaatcg aacagggcat
     6121 cgatcagcac cgccagaaca attaccggga tcacccccag caacactaaa tcaatggcgc
     6181 tgcttagcag cccctggaaa accagcgcac caaaaccgcc tgcgccgatt aacgccgcaa
     6241 tcaccgccat acctacagtt tgcaccatca ccacccgcag gctgcgcaga aataccggta
     6301 acgccagtgg taactgaaca tgcaggaatc gctgcgcccc gctcatgccc atcgctctgg
     6361 cgctctccag cacatcgcgc gggatctggt tcaaaccgac taccacgccg cgcaccagcg
     6421 gcagcaaggc atagagcacc agcgcaatca gtgcgggtgt cattccggtt cctgctatgc
     6481 cgagcttccc cagccacgga aaggccgtca ccagcgcggc aagcggcgca atcaacaggc
     6541 caaagagcgc caccgaaggc acggtctgaa tgacattgag cagagaaaaa attgccccct
     6601 gccgagcagt ggaaaagtag caccagatgc ccaacggcac accaatcact aacgcaggca
     6661 gcaccgcacc aaacagcaac gtcagatgtt gtgccagcgc gtcgtcaaac acatcctgac
     6721 ggttggcgta ttctttcatt agtgagagat cgttaagcgt gccggagtac agcaaccaca
     6781 gcggaataat ggcaatctgc atatgcaaca accagcgcca cagcggatgc gtggagattc
     6841 ggcggatggc atcgctacag gccagcaatg ccagcgccgc agccagccag aaaccactgc
     6901 cgaggctggt acgcgccagc gcactgccat tttgcgccag ttgtgtcgcc gcctttcccg
     6961 cgccccacac cagcaatacg aagacgaatt gcgccagaat gagtgcacaa atgctgcctt
     7021 ttttaccggg aataaaacag gccgtcagcc aggcgcaacc aacgcccacc agcatccaga
     7081 tcgtttgcgg ccacagttgc cagagatgac gcccttcacc cgagactaaa cgattaggcg
     7141 cgtagctgat aaacggcagc gctgctgcga ttgccgtcag caacagcagc agcgccagaa
     7201 caggattaat acgtagataa gtcacgggaa attacttcgc ccaccctttt tgtttcaggt
     7261 agtcggcagc cacttttttg gcatccagtc cttccactgc aatgctggca ttcagttgct
     7321 gcaatgtttt tgcatcgagg ctggcgaaga ctggctgtag ccactgtgcc atttgcggat
     7381 actccttcaa caccgattca cgcaccactg gtgcaggcgc atagataggt tgcacgcctt
     7441 gcggatcgct taaggtttgc agccccagcg ccgctaccga gccgtcagtg ccgtaagcca
     7501 ttgcagcatt aacgccagag gtttgctggg cagcggcttt gatcgtcacc gccgtgtcgc
     7561 caccagccag tgacagcaac tgatcctgac cgagcttaaa gccataggct ttttcaaacg
     7621 cgggtaacgc atcggcgcgt tcgataaact ctgccgaggc tgccagtttg aaggtgccgc
     7681 cctcttgcag ataacgactc aggtcggcaa gcgaagtgag tttatttttc tctgccacat
     7741 cctgacgcac ggcgatagtc caggtgttat ttgcaggcgc gggcgtcagc cagattaact
     7801 tgttgtgctc cgaatcgagc tttttgactt tctcgtaacc ttgctgcgcg tttttccacg
     7861 ctgcatcgtt ttcatcttta aagaagaaag cgccattgcc ggtatattcc ggatagatat
     7921 ccagttcacc ggaagtaatc gccccgcgca ccacaggagt cgttccgagt tgcactttat
     7981 tcaccgtcgg tacgccgtgg ctttccagca cctgcaaaat gatattgccg agtagcgcac
     8041 cttcggtatc gatttttgaa ccgactttaa cgggggaagc cgcctgtagc ggcaggctca
     8101 cggctgccaa cataaccagt gaacctgccc agagctttaa gagtggcatg atgctttcct
     8161 cattctttta ctgttgtttt cagcgaatta agagaaaagc atagttgata atggcggggt
     8221 tagcttgagc ggaaggattc agttgcagaa tcagataaat acttaagagg catattcggt
     8281 ccggcgtttc ctgccggatg cggcgcgagc gccttatccg gcctacaaag ggcgcaaacg
     8341 tcgcgccctt actaaagcat tacagcaact caaactcgcc tttcttaacg cgtgcggaat
     8401 cagtgccgat aaatacattg aacttgccag gctcggcgtc atatttcatc tgttgattcc
     8461 agaacttcag cgcctcaata tcgatcggga agctgacagt ctgagtttcg cccggtttca
     8521 gggtgatttt ctcaaagcct ttcagctgtt tcacagggcg actcatggaa gccgtcacat
     8581 cctgcaagta catctgcact accgtggccc cctcgcgctt accggtgttc gtcacctgca
     8641 cgctggcagt cactttgccg tcacgcttca tggtcggcgc agaaagtttc acatcagaga
     8701 cggtgaaagt ggtgtagctc agcccatagc cgaacggata caacgccccg ttagcttcat
     8761 caaaataacg cgaagtgtat ttgttcggct tgtcggcatt atacgggcga ccggtattca
     8821 gatggctgta gtacaccggg atctgcccga cagaacgcgg gaaggacatt ggcagcttgc
     8881 cggacgggtt gtaatcgcca aacaatacat cggcaattgc attaccgcct tcagtccccg
     8941 caaaccaggt ttccagaatc gcatcagcct gctgatcttc tttcaccagc gccagcggac
     9001 gcccgttcat cagcaccagc accagcggtt taccggtggc tttcagcgcc gcaatcaagt
     9061 cacgttggct ttgcggaata gtgatatcgg tccggctgga ggcttcgtgc gccatcccct
     9121 gtgcttcacc gactacagcc accaccacat cagattgttt cgccgtctgc accgcttcat
     9181 caatcatctc ttgcggcgaa cgcggatcga ctttgaccgc ttcttcatac tgattcagga
     9241 aatcgataat gcctttgtca ctggtaacgt tcgccccttt ggcatacagc actttaccgt
     9301 tttcaccgac ggaattttta atcccggtca gtacggtcac ggattgatcg gcaacaccgg
     9361 ctgcggacca gctgcccatc acgtcacgtt tactgtccgc cagtggccca accaccgcaa
     9421 tggtggccga ttttttcagc ggtaacgttt cgagacggtt tttcagcaac accaggcttt
     9481 cgcgcgccac ttcacgcgct tctttacggt gcaggcggct ttcggcattg gtatccaccg
     9541 ggtcagactc tttcggcccc aaatggctgt atgggtcgtt aaacaatccc atatcatatt
     9601 taacgttcag tacatggcgg gcagcatcgt ccagctcttc catcgtcact ttgccggatt
     9661 tgatcaaccc aggcagatac ttcgagtaat actcgtcgct catactcatg ttgattccgg
     9721 atttcagcgc cacgcgcacc gcatcttccg ggtctgccgc cgtgccatgt ttaatcagct
     9781 ctttgattgc accgtgatcg gaaacggtga tgcctttaaa gccccactgg tcgcgcagaa
     9841 catctttcag cagccaggaa tcggaggtgg ctggcgtgcc gttcagcgag ttcagcgcca
     9901 ccatcaccgc gccgctgcct gcgtccagcc ccgctttgta cggcggcata taatcattaa
     9961 acaggcgctg cggactcata tcgacggtgt tgtactcttt accgccttct accgcgccgt
    10021 atgcggcaaa gtgtttgacg ctggtcatca ccgagtagcg atctgccggg cttttaccct
    10081 gcatcgcttc caccatggtt ttacccattg ttgaggtgag atacgtatct tcgccaaaac
    10141 cttcggaagc acgtccccag cgcggatcgc gcgagacatc gaccatcggt gcccaggtca
    10201 tattcaggcc atcatctgcc gcttcataag cagagacacg tccgaccgtt ttcactgcat
    10261 cgaggttaaa agacgaggcc agaccgaggc taatcgggaa caccgtgcgc tgaccgtgca
    10321 gcacgtcgta agcaaagaaa agaggaattt tcaggcggct taattccatc acctgatcct
    10381 gcatggcgcg gatatcctga cgggttacgg tgttgaaaat cgccccaacc tgaccgtctt
    10441 tgatcatctc gcggatcgcc tctttcgggt tatccgggcc gacgctgatt aagcgcagct
    10501 gaccaatttt ctcatcaact gtcattttct taagcagttc ggtgacgaac gcatcccgcg
    10561 cttcgggcgt taatggatgg ttgccgaata aatcatccgc cagtgctggc tgcagggcca
    10621 gactcaccgc gattcctact gaacatagcc atttcatatg gatttttctt cctctgttgc
    10681 ccgacgaggc agcgaaaatg gtgcaaaaac cgtagtttgc cataagcatg atggagagag
    10741 aaaaagaatg ctcagtttat tgtctgaatt ttcaaaatat tcactcgctg aattgtcata
    10801 caaggcgcta ttctagtttg tgatattttt tcgccaccac aaggagtgga aaatgtcttc
    10861 catgacaaca actgataata aagccttttt gaatgaactt gcccgtctgg tcggtcattc
    10921 acacctgctc accgatcccg caaaaacggc ccgctatcgc aagggcttcc gttctggtca
    10981 gggcgacgcg ctggctgtcg ttttccctgg ctcactactg gaattgtggc gggtgctgaa
    11041 agcctgtgtc accgccgaca aaattattct gatgcaggct gccaatacag gcctgaccga
    11101 aggatcgacg ccaaacggta acgactatga tcgcgatatc gtgatcatca gcaccctgcg
    11161 tctcgacaag ctgcacgttc ttggcaaggg cgaacaggtg ctggcctatc cgggcaccac
    11221 gctctattcg ctggaaaaag ccctcaaacc gctgggacgc gaaccgcact cggtgattgg
    11281 atcgtcgtgt ataggcgcat cggttatcgg cggtatttgt aacaactccg gcggctcgct
    11341 ggtgcaacgt ggcccggcgt ataccgaaat gtcgttattc gcgcgtataa atgaagacgg
    11401 caaactgacg ctggtgaacc atctggggat tgatctgggc gaaacgccgg agcagatcct
    11461 tagcaagctg gatgatgatc gcatcaaaga tgacgatgtg cgtcacgatg gtcgtcacgc
    11521 ccacgattat gactatgtcc accgcgttcg tgatattgaa gccgacacgc ccgcacgtta
    11581 taacgccgat ccggatcggt tatttgaatc ttctggttgc gccgggaagc tggccgtctt
    11641 tgcggtacgt ctagatacct tcgaagcgga aaaaaatcag caggtgtttt atatcggcac
    11701 caaccagccg gaagtgctga ccgaaatccg ccgtcatatt ctggctaact tcgaaaatct
    11761 gccggttgcc ggggaatata tgcaccggga tatctacgat attgcggaaa aatacggcaa
    11821 agacaccttc ctgatgattg ataagttagg caccgacaag atgccgttct tctttaatct
    11881 caagggacgc accgatgcga tgctggagaa agtgaaattc ttccgtccgc attttactga
    11941 ccgtgcgatg caaaaattcg gtcacctgtt ccccagccat ttaccgccgc gcatgaaaaa
    12001 ctggcgcgat aaatacgagc atcatctgct gttaaaaatg gcgggcgatg gcgtgggcga
    12061 agccaaatcg tggttggtgg attatttcaa acaggccgaa ggcgatttct ttgtctgtac
    12121 gccggaggaa ggcagcaaag cgtttttaca ccgtttcgcc gctgcgggcg cagcaattcg
    12181 ttatcaggcg gtgcattccg atgaagtcga agacattctg gcgttggata tcgctctgcg
    12241 gcgtaacgac accgagtggt atgagcattt accgccggag atcgacagcc agctggtgca
    12301 caagctctat tacggccatt ttatgtgcta tgtcttccat caggattaca tagtgaaaaa
    12361 aggcgtggat gtgcatgcgt taaaagaaca gatgctggaa ctgctacagc agcgcggcgc
    12421 gcaataccct gccgagcata acgtcggtca tttgtataaa gcaccggaga cgttgcagaa
    12481 gttctatcgc gagaacgatc cgaccaacag catgaatccg gggatcggta aaaccagtaa
    12541 acggaaaaac tggcaggaag tggagtaaaa attacggatg gcagagtatc gccatccgaa
    12601 ttcacttaat cgttctgtgc cgtctgcccc gccgccgcca tttgggcggc tttttgtttt
    12661 ttatagctca acgctgctgc cggcacaggc atcactttac cggtttcaat ccaggtacgc
    12721 aggcggctgg catcggcaaa atgggtatat ttgccaaacg cgtccatcac taccagcgcc
    12781 accggtttat tattgataac cgtacgcatc accagacaat ggcccgccgc attggtaaag
    12841 ccggttttgg ttaactgaat attccagtta tcgcgataca ccagatgatt agtattgcgg
    12901 aacggcagcg tatacgtcgg gttagagaag gttgccatat cttcccgggt agtacttaac
    12961 tgcccgatca acggatactg tttgctggca atgagcagtt tggttaagtc acgggcagtt
    13021 gaaacgttat gcaccgacaa tccggtaggt tcaacaaagc gcgtgttgtt cattccgagc
    13081 gatttcgctt tcgcattcat tgccttaata aaggctttgt aaccaccggg ataatggtgc
    13141 gcaaggcttg ccgccgcgcg gttttctgaa gacatcagcg ccagcaacag catatcttta
    13201 cggctgattt cgctattcag tcgtacgcgc gaatagaccc ctttcatctc cggcgtctgg
    13261 ctgatatcca cttttagttt ttcatccagc ggcagtcgtg catccagcac aaccatcgcg
    13321 gtcattaatt tgctgataga cgcaatcgga cgcaccagat ccgggtggtt cgaatagatc
    13381 actttgttgg tattcagatc aacaatcatc gcgctaccgg aggcaatttc cggttgtgaa
    13441 gcggtggtag cggctgccgt tttcgcaacg gcctgcggtg ccaaaggcac agccagcatc
    13501 agggccaggc taaataaaga aactcgaaat ttcggcatga tgagcattca gatagtggtt
    13561 cacgcgcacg ggttgcgcac cgccggagta aggatttact gaggctagcg acgccatcat
    13621 aacgagcaaa aagtgcgatc gtcaaaggag aatcgtgagg aaatgctgca ttgctgacat
    13681 ttacgccagc aatgcaatgt caaagaactt tctagaacag gcgatacccg tagccccata
    13741 gtataacggt tagggcgagc agcacttcca gtaccagcac gccaatcgcc agcgtcgaac
    13801 tggagaagct aaggccttcc tctttgttga tattcaggaa gctcggaata ccaaggtaaa
    13861 gcaggtagcc ggtgtaaaac agcgccaccg tgccgaccag cgcacacaac cagaccagcg
    13921 gataaagcgc caccagaccg cttaaaaaca gcggagttgc aacgtagccc gcgaagacca
    13981 tgcagtgcgc aagtgacgga cgctgcgggt aattgcgcgc catccaccag atgacccgcc
    14041 ccatcaccgc aaccccagcg agcataacgc cataaaacag gacagccagc gccagtccgg
    14101 taaaccagga caacttcagg atagtgccat cgccaaaatt ccagccaatc tgtgtagtgc
    14161 caatgaaggc gcaaatcacc ggaatcgccg ccatcagcaa aacgtggtgg gtgtaatggt
    14221 gagaaatcgt ttcgttttcg cgattaatca cctgcatttc acgatcggga tgggaaaaca
    14281 gtccccagac atggctcata ccgccccctt gttgtgagtt catgaacctg acagttcaag
    14341 tataagtcag cttgtgatta ttttttgttc gctcatgtaa tttgcaccgt ctgatgtacg
    14401 tcctttttca gcgtgaatga tttccagggt gtatgcttac aggcaaccaa agggagacag
    14461 actggcctat ggatctcaat acactcatct cacaatatgg ttatgccgcg ctggtgatcg
    14521 gtagcctggc ggaaggtgaa accgtgactt tgctgggagg cgttgcggcg catcaggggc
    14581 tattaaagtt cccgctggtg gtactttctg tggcgcttgg cggcatgatt ggcgaccagg
    14641 tgctttatct gtgcgggcgg cggtttggcg gcaagctgtt acgccgtttc tcgaaacatc
    14701 aggataaaat tgagcgggcg cagaaactta tccaacgcca tccgtatctg tttgtcattg
    14761 gtacgcgctt tatgtatggc tttcgggtga ttggcccgac gctgattggt gccagccagc
    14821 tgccgccgaa aatctttctg ccgcttaata ttctcggcgc atttgcctgg gcgttgattt
    14881 ttaccactat cggttacgct ggtggtcagg tgattgcgcc gtggttgcac aatctcgatc
    14941 agcatttgaa gcactgggtc tggttgattc tggtcgtggt tctggtggta ggcgtgcgct
    15001 ggtggctgaa acgacgcggg aagaaaaagc cggataatca ggcgtaaaac cattgccctg
    15061 gataaggcgt tcgcgccgca tccgacatcc ggtgcaccat gcctgatgcg gcgcttgtgc
    15121 gtcttatcat gcctaccaaa attttaccca ttgaaatatc cacacacctc actgcgctat
    15181 tctgggttga actgcggatt cgccaacata aagccgccat ccactatcaa cgactgcccg
    15241 gtggtgtaat ttgcgccctc cgaacaaagc cacgccacca ggctggcaat ctcatacgtt
    15301 gcgccaaaac gccgcagagg aatcgaaggc tccgcgtcgg gcttcacgtc gctatcatcc
    15361 atgccgttca ttggcgtagc gatcgcccca ggtgcgactg cgttcaccaa aatcttatgc
    15421 ctgaccaact ccagcgccat tgctttggtt aacccgccga gcgcatgttt agcggctgtg
    15481 taggcgctgg catccggcag cggcgtatgt tcatgtaccg acgtaatgtt gatgatgcga
    15541 ccaccctgcc cttgtttcac catctggcgg gccgcaattt gtgagcataa aaatgcacca
    15601 tcgacatcaa cggtaaaaat cttgcgccac tcatcgaaag ccatatcaag aaacggcgct
    15661 ttggtcattg cgcccgcatt attcaccagc acatcaatac gccccagccg ttgaatgagt
    15721 ttctccagcg cctgtgcacc ttctggcagt ttaccgagat ccagctgcac aatctccgca
    15781 cgtacgccgt ggctaactac ctcacgcgcg gtatcttttg ccccttcttc atctgagtgc
    15841 caggtaatgc caatatcaaa cccctgctgc gccagtaata acgcgcactc tttgccgatc
    15901 cccgaatcgg aggcggtaat aatcgcaacc tgtgccatcg agttctccac ttaacgctga
    15961 ataaacgtta agtatagaag gcgcatatca tcagcgtttg taacccccgc ccaacgcacc
    16021 agtgagttga atggaagcat ccagccactg cccttgcaat aacaggccat tggcccgctc
    16081 acgcagcgcg gggattctgg cttcgctgac gcgggaacca gcaatgatgc ccgcgttaaa
    16141 gcgcgcctgc gcaagaccta ccacacgcaa ggcatcgcgc tcaatttgcg cctgatgctg
    16201 gtttttctcc gcaagtgtct gaacctgact ggctgcccgc gccacgtcat tcaccgcttc
    16261 aaccaccgct ttgttgtagc tggcgataga caagttgctt tcggcttttg cgatatcgag
    16321 attggcgtta agacgaccac tatcgaaaat gggtagcgtc aggcctgccg taacgcccat
    16381 ttgctgcgcg gaatgacgga acagatcgct taagtgcaac gcatcctgtt gcaggaaggc
    16441 catcaggttg atgtcaggat aaaatgccgc ttttgccgca tcaatggtgc ttagcgatga
    16501 ctcaacgtac cagtgcgccg cctgcaaatc tgcccgccgg gccagtaagg agtaccccag
    16561 ttcatcagga agctggcttg ccactttcgg caacgcgacc ggtttaagct tcaatgactt
    16621 tgtctggtta tttgtaagtg cgcttaaccg tgcctcaata attttcattt tccccgcgac
    16681 atcgttgagc tgctgccggg ttttgctggc attaatatcg gtttccacac cttcaactga
    16741 agaagtaatc ccgttctgat atagctggcg atcggtcgcg ataatggtgt tctgctcttt
    16801 ttctatttgc tgcaagaccg tgtttaacgc cgcctgggtt tgccactccc agtacaggcg
    16861 ggctacgctg ccagccagca attggcgggt ttgctcgcgt tccgccgccc gtgctttaac
    16921 cgtacccagg cgggcagtaa cctccgcccg attctttccc cagatatcga gatgccagcc
    16981 cgccgttaag ccaaaagttc cgttggtgta ccacggaccg gtcgtacctg cggccggatc
    17041 gttcagagca aacggcccca ttaagccttc tgccgacatt ttttgccgct ccatatccgc
    17101 agaaaagtcg atctgcggac catcctgagt agcaattgcc ttcgcctggg cttcagctag
    17161 ctgaatgcgc tgttcagcca cctgcatatc cggtgcgttc tgtagtgcat tgttaattaa
    17221 ggaagtgagt tgattatcgt gatactccag ccaccattgg ctgtctggcc aaccattttt
    17281 cagcgccgtg ggtaatgcgg tgtcaacttg tgcagcgggc gtttgctggc ttaacgcctg
    17341 gcgggtttca tgcataggcg cacacccggc cagcatcagt aacagcggaa aacaggcgat
    17401 ggcgggataa aaggaatcac gattcatggg ggaataatca ggtaagaaaa ggtgcgcgga
    17461 gattaccgtg tgttgcgata tattttttag tttcgcgtgg caatacatca gtagcaataa
    17521 aacgacatat ccagaaaaat atacactaag tgaatgatat cttccgattt atcttaatcg
    17581 tttatggata acggcaaagg gcttcgtttt ttcctatact tattcagcac tcacaaataa
    17641 aggaacgcca atgaaaatta tactctgggc tgtattgatt attttcctga ttgggctact
    17701 ggtggtgact ggcgtattta agatgatatt ttaa
//
LOCUS       DAZGLJ010000090        17666 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000090 DAZGLJ010000000
VERSION     DAZGLJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.090"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            32..748
                     /locus_tag="ACHK49_002600"
     CDS             32..748
                     /locus_tag="ACHK49_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO9263229.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            778..1278
                     /locus_tag="ACHK49_002601"
     CDS             778..1278
                     /locus_tag="ACHK49_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFO9263230.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQITKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1370..4054
                     /locus_tag="ACHK49_002602"
     CDS             1370..4054
                     /locus_tag="ACHK49_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFO9263231.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADISDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDGLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4051..5125
                     /locus_tag="ACHK49_002603"
                     /pseudo
     CDS             4051..5125
                     /locus_tag="ACHK49_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
     gene            complement(5213..6511)
                     /gene="exuT"
                     /locus_tag="ACHK49_002604"
     CDS             complement(5213..6511)
                     /gene="exuT"
                     /locus_tag="ACHK49_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO9263232.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            6994..8406
                     /gene="uxaC"
                     /locus_tag="ACHK49_002605"
     CDS             6994..8406
                     /gene="uxaC"
                     /locus_tag="ACHK49_002605"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO9263233.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8421..9908
                     /gene="uxaA"
                     /locus_tag="ACHK49_002606"
     CDS             8421..9908
                     /gene="uxaA"
                     /locus_tag="ACHK49_002606"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO9263234.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9991..10542
                     /gene="ygjV"
                     /locus_tag="ACHK49_002607"
     CDS             9991..10542
                     /gene="ygjV"
                     /locus_tag="ACHK49_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO9263235.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10547..11791)
                     /gene="sstT"
                     /locus_tag="ACHK49_002608"
     CDS             complement(10547..11791)
                     /gene="sstT"
                     /locus_tag="ACHK49_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO9263236.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12190..13155)
                     /gene="alx"
                     /locus_tag="ACHK49_002609"
     CDS             complement(12190..13155)
                     /gene="alx"
                     /locus_tag="ACHK49_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO9263237.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(13438..14424)
                     /gene="ygjR"
                     /locus_tag="ACHK49_002610"
     CDS             complement(13438..14424)
                     /gene="ygjR"
                     /locus_tag="ACHK49_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO9263238.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14503..15182)
                     /gene="ygjQ"
                     /locus_tag="ACHK49_002611"
                     /pseudo
     CDS             complement(14503..15182)
                     /gene="ygjQ"
                     /locus_tag="ACHK49_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
     gene            complement(15259..15762)
                     /gene="ygjP"
                     /locus_tag="ACHK49_002612"
     CDS             complement(15259..15762)
                     /gene="ygjP"
                     /locus_tag="ACHK49_002612"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO9263239.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTPDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15847..16983
                     /gene="rlmG"
                     /locus_tag="ACHK49_002613"
     CDS             15847..16983
                     /gene="rlmG"
                     /locus_tag="ACHK49_002613"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO9263240.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSISDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            17174..17257
                     /locus_tag="ACHK49_002614"
                     /pseudo
     CDS             17174..17257
                     /locus_tag="ACHK49_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            17254..>17666
                     /gene="higA"
                     /locus_tag="ACHK49_002615"
     CDS             17254..>17666
                     /gene="higA"
                     /locus_tag="ACHK49_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO9263241.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFI"
BASE COUNT         4686 a         4357 c         4492 g         4131 t
ORIGIN      
        1 tttggaaaca atattgtaag atcaaattta tatggtggcg aaaattaatc tcttatcacg
       61 gcttacgccg cttttattcg tgttcgctcc ttttttggca cagtcaaata tgactgttta
      121 tcctatggct gtttccatta atagtcaggg tgaaggcaac gttcgcgtaa tatctaaaag
      181 taatgaagtt cagtacatca aggcgacagt attccgtatc gataatccat cgacgcctca
      241 ggaaaatgaa gttgagatta aatccggtga tgcaaatcat ctggttgtta tgccacctaa
      301 attcgcttta ccagccggta gtagcaaaac cgtacgtttt gttgcgatgg aaccagagca
      361 aaaagagaaa aattatcgcg ttaaatttga agcggttccc agtattgatg acgttgccac
      421 agataaaaaa gatctctcta tgcagttaac agttaactta atttggggga ttgttgttag
      481 tgttccacct cagcaaccta ttgctaagtt agaagtaaat gctgcccaaa aattagttaa
      541 tgcagggaat caacgcttaa agattttaac gatcgcttat tgtaaaaata atagcaaaga
      601 aaattgtaag atacagaccg taaataaaaa catcttccct ggtcaggaaa gaaatcttga
      661 aagcatatct ggctacgaca aaatcgttgt caaatataat aactggatca ccaaagataa
      721 tggcgagttt gaactggcgg tccattaatt atcattgtaa taaacgaagg aatagtaatg
      781 aaaaaggtat ttgcaaaatc tcttctggtc gcagcgatgt tttctgttgc aggctccgca
      841 ttggcagtac aaaaggatat tactgtaacg gccaacgttg atgccgctct ggatatgaca
      901 cagaccgata acaccgcgtt gccgaaagca gtagaaatgc aatatctgcc ggggcagggt
      961 ctgcaatctt accagttgat gaccaaaatc tggtctaacg acgttaccaa agatgtaaaa
     1021 atgcagttag tttccccggc gcaactggtt caaagcctgg atgctagcaa gattgttccg
     1081 ctgacagtaa cctggggcgg tgaagaaatt aaagctgatg cggcaaccac ttttaccgca
     1141 accaaaatct ttgcttccga tgcgttaact aacggttctc tggctaaaaa cctgatgttt
     1201 gctcaaatca ccaaaggtgt tctggagaca ggcatctacc gtggtgtagt aagcatttat
     1261 ctgtctcagg atatctaatc cagataacag aattaacacg taacaaaggg aggggtagcc
     1321 ctcccttatt ttaactgttt tagtatttgt tatggatttc aggattgcca tggataaaaa
     1381 attactggct cttttgatcc tggcgagtct cagcccggca gaggcgacat taaccaaaat
     1441 tcccgcaggg tttgaggtta ttgctcaggg acagcaggag tatatcgagg tttatttttc
     1501 agggaaaagt ctcggtaaat attatgcaat ggttaatctt gataccgtaa catttcttga
     1561 tccagcaagt ctatataaca agctggaact ggatgtagac gatcagaaaa tcgcgcatat
     1621 agtgaaagaa aaattatcgc agccgctagc tcgccacggt gaattggctt gcggttatgt
     1681 acgtactgac tcagggtgtg gttttctgaa taccgatacg ctggaaataa tctataatga
     1741 tgaagaaagt tcggcaacgt tgtttattaa tccgcaatgg aattcagctt tcgatgcgaa
     1801 gtcattatat ttaaatccag acaaaaatac tgttaatgct tttatacatc agcaagacat
     1861 caatgttctg gcacaggatg attaccaatc gttgtctatt cagggaaacg gtgcgctggg
     1921 aataacagaa aatagttata ttggtgcaca ctggaatttc aacggttatg atgcagatga
     1981 tgtcagtgac agtaatgctg acatcagcga tctctattat cgttatgatt ttttacgtcg
     2041 ttattatgtg caggcgggtc gcatggacaa ccgcacacta tttaatgcac aaggcgggaa
     2101 ctttaccttt aactttctgc cactcggtgc aattgatggg atgcgtatcg ggtcgacact
     2161 gagctattta aaccaggcgc aaagccagca aggaacccca gtaatggttc tgctttcgcg
     2221 caattctcgt gttgacgctt atcgtaatga gcaacttttg ggatcgtttt atctcaatag
     2281 tggttcgcaa tttattgata ccagttcctt tccgccaggc agctacagcg tggcattaaa
     2341 agtctacgaa aataaccaac tcacccgcac cgagcttgtg ccgtttacca aaaccggcgg
     2401 tctgactgac ggaaatgcgc aatggttctt acaggcaggt aaaactacat cacaggtttc
     2461 tgatgatgaa agttcagctt atcagctagg agtacgcctg ccattacatc cgcaatatga
     2521 gctctacgca gggctggcga atgccgatga tgtgagtgct ttcgagttag gtaataactg
     2581 gacggcagat ttaggcgggg tggggaatct tgcaatcagc gccagcgtgt tccgtaacga
     2641 tgacggcggc aaaggtgata tgcaacaggc caactggagt aatccgggat ggccgacgtt
     2701 gggcttttat cggaccaact ctgacggcga tgcttgtaca accgacagca gagagagcta
     2761 taacgcctta agctgttatg aaagtatttc cgcgacggtt tcacagaatt ttgtcggctg
     2821 gaatatgatg ctgggttata cccgcacaca aaataacact gatgatagtt tgcgttggga
     2881 taaacagcag agctttgaaa ataactatct tcgccagaca acagcgcaaa gtatctctga
     2941 aactgtacaa cttagcgctt cccgcgcttt tgtgatgcgt gactggattt tgagtacgtc
     3001 agtcggtgtt ttccatcgta atgacaacgg tggcgataac gacgacaacg gcttgtactt
     3061 atcgttttcg ttatctgaca cgccaacgat ggatagcaat aacaacagcc attcaacaaa
     3121 tgtgtctacg gattatcgtt atagcgaaca ggatggcgat caaacgtcat ggcagttatc
     3181 gcataccttt tataacgatt cattcagcca taaagaactt ggcgtaaccg tcggaggcct
     3241 gaacaccgat accataaaca gcgcggttaa cgggcgttgg gatgggcaat acggaaatgt
     3301 ctacgctacc gtatctgata gttatgaccg taagaatcat gatcatcttt cggcctttac
     3361 gggcacttat agctctacgc tggctgtcag tcgctatggc gttaatttgg gagccagtgg
     3421 tacagacggt ttgctgggtg cggtactggt ggatgtgaaa ggcttctctg aacaggatga
     3481 agagagtcag gatctgcaac tcgaagcgcg ggtggcaggc agccgaactt tgcagcttgg
     3541 tcaaagtgac agtgtgttgt tcccttatcc tggatttcag tctggttttg ttgaggttaa
     3601 cgacagtagc cagggcaatc agcaggggac aacaaacatc attaacggtg cagggaatcg
     3661 tgaattaatg ttgttgcctg gcaagctgcg ctatcgcgaa gtgtctgcca gctttaacta
     3721 caactatatc ggtcgtttat tactgccggc agcggtgaaa aaattcccga ttgtggggct
     3781 gaacagcgcc atgttactgg tagctgaaga tggcggattt acacttgaaa ttaacggcag
     3841 cgaaaaagag ctctatctgc tttccgggca gcaattcctt aagtgtccac tgagtgttgt
     3901 aaagaaacgc gccagcattc gttacagcgg agatgttact tgtagtgtgg tgacttattc
     3961 acaattaccg gagtcgattc aggttcaggc acagttgaaa cagcctaaat tacgtggaaa
     4021 cgttcagacg gcgcaaaggg aggttgcacc atgagaaatc gattgattgc ggcgatattg
     4081 ggcttgtttg gttcaggcag ctcctgacgt gaccagtgaa attacgtatg atttggcatc
     4141 tggtagagcg gattattact tctggaagga tgaagcgtcg gcaggaaata atggatatat
     4201 gtggtatgaa tgttcgtatc ctgacctcca acaaacctgt acagctaatg gaaatatatc
     4261 gacagtacaa atctatttaa ctgaacaacg cagtgggatg cgttggccgg taaaactcaa
     4321 aggatttaaa acagccattg taagtagcga tgaagcgccg ccaggatgca aggggggcaa
     4381 agggcttcag acgaatctta aggattctaa tagatcttca tgtacagaag atggtcaaca
     4441 ttattatata tacgatacaa agtttcttac gctctacctt gagcagacag agatgaagaa
     4501 tttgccgatt ggtggcgtct ggaaggggaa agttaaatta cattcgaaca gcccggccca
     4561 ggactatttc gcaaatatta ccctgaatac gctcgacccc aaccatattg acgtgttctt
     4621 cccggagttc gcccacgcca cgccaagggt gcagttagac ttgcatccaa caggaagcgt
     4681 taacggcagc aactacgcgc aagatctgac catgttggac atgtgcctgt acgatggttt
     4741 taacggtaat gccatcagtt atgaaatcat gctcaaagat gaagggcgac cagctgcagg
     4801 gcgcagagac ggttacttct ctatctatcg tcagggaggg accaccaccg acgagggaga
     4861 acgcattgat taccgggtca aaatgtacaa cccggaaacc ggtgggcaaa ttgatgtgcg
     4921 caataatgaa aatatggtct ggaacagcat taacctgaaa cgtgtgcgtc cggtggtact
     4981 gcccggtatt cgctatgccg tgatgtgtgt gccgacaccc ttgactctgg cagtcgataa
     5041 attcagcgtg atggataaac aggccgggta ctacatgggc aaattgtcag taatctttac
     5101 gccttccttg ccaaccatca attaaccctg caaaccggac aaacgctttt ttctatataa
     5161 acgacgaagc cgtctgataa agcggcttcg tcattacaac ttaagttaaa cgttaatgtt
     5221 gcggtgcggg atcgttatgt gtttcctgcg ccacctcaat cgccggtttg ttctgcaaca
     5281 cggtccagat aaccagcgca cctaacaggt cgaacactgc cagaactgcg aacagcgggc
     5341 tgaagccgat ggtgtcagcc agtgcaccga caaccagcgc aaacagcgta cttgccagcc
     5401 atgcggacat cccggttaaa ccgttcgccg tcgccacttc gttacgaccg aacacgtcag
     5461 aagagagcgt aatcagcgcg ccagacagtg cctggtgggc aaaaccaccg atacacagca
     5521 gcataattgc gacatacggg ttggtgaaca gaccgatcat acccgggcca atcatcagca
     5581 cagcacccag cgttacgacc atcttacggg aaacgatcag gttcacacca aaccaacgct
     5641 ggaacagcgg cggcaggtaa ccaccgagga tacaaccgag gtcagcaaac agcatcggca
     5701 tccaggcgaa catcgcgatc tctttcaggt taaagccgta aactttaaac atgaacagcg
     5761 ggatccacgc gttaaaagta ccccaggccg gttctgccag gaaacgcggc agcgcgatac
     5821 cccagaactg acggttacgc aggatctgac caacggacat tttcttcgcc gtgctcacct
     5881 ggtgctgggc ttcctgacca ttaataatat agtcgcgttc ttcatcggtc agatgcttct
     5941 ggtcgcgcgg atgtttatag aaaatcagcc atgccatcgc ccagataaag ctcaatgcac
     6001 cggagatgat aaatgccatc tgccagctgt gcattacgat tgcccatacc accagcggcg
     6061 gcgcaatcat cgcaccaatc gaagaaccta cgttaaagta acctaccgcg atggaacgct
     6121 ctttcgccgg gaaccattcg gagctggctt tcagacccgc cgggatcatc gctgcttccg
     6181 cggcaccgac tgcaccacga gcaatcgcca ggccacccca gctaccagcc agcgcggttg
     6241 caccacagaa cacggcccac agcacagcaa acattgcata accgattttc gtacccagca
     6301 catccagtac ataacctgct accggttgca tgaccgtata agcagcagaa taggctgcga
     6361 tgatatagga atactgttgg gtggagatgt ttaactcttc catcagagtt ggcgcagctg
     6421 ccgccacagt gttacgcgtc aggtaaccaa gcacggtgcc gagcgtcacc agtgcgatca
     6481 tataccaacg taacccttta attttacgca tgtaaaacct catcgttttg ttatatccgc
     6541 aggaagcgcg gccatcatcc cgccagaggc tgaagatgtt agttacgacc gggtaaccga
     6601 aaaaaaaccg gcacgccccg aacgttgcca tgaaagtttc gtgctaattc ggcttccgta
     6661 ccggttaacc cgattcatct aacaaatgaa tgaatcggca atttgcattc cgtcgactta
     6721 tgatttgcga cggcagaaag ataacttgtc atacaacttt aaaaggtgag agccatcaca
     6781 aatgtgcgaa tatttgtagg gacattacct gacgacagcg aggccagtac tggcgcggcc
     6841 tgcagagaga tttaccactt tgagagtaat ttttttaact acgtttattg atctaactca
     6901 cgaaaatatc ttcggactct ggaaattggt gtgataactt tgtcagcatc gcaccataag
     6961 caagctagct cactcgttga gaggaagacg aaaatgactc cgtttatgac tgaagatttc
     7021 ctgttagata ccgaatttgc ccgccgtctg tatcacgact acgcaaaaga ccagccgatt
     7081 ttcgattacc attgccattt gccgccgcag cagattgcgg aagactatcg ttttaaaaac
     7141 ctgtatgaca tctggctgaa aggcgatcac tacaaatggc gcgctatgcg taccaacggt
     7201 gtggccgagc gtctgtgtac cggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg
     7261 gcgactgttc cgcacactat cggcaacccg ttataccact ggacgcacct cgaactgcgt
     7321 cgtccgtttg gtatcactgg caaattgctt tctccgtcaa ctgccgatga aatctggaac
     7381 gaatgtaacg aactgctggc gcaggataac ttctccgcgc gcggcatcat gcagcagatg
     7441 aacgtgaaaa tggtcggcac caccgatgac ccgatcgatt ctctggagca tcacgcagag
     7501 atcgccaaag acggctcttt caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc
     7561 ttcaacatcg aacaggcgac ctttaacgac tacatggcga agctgggcga agtttccgat
     7621 accgacattc gccgctttgc tgacctgcaa actgccttga ccaaacgtct ggatcacttc
     7681 gccgctcacg gctgtaaagt gtctgaccat gcgctggacg tggtgatgtt tgctgaggcg
     7741 aacgaagcgg aactggacag cattctggcg cgccgtctgg ctggcgaaac cctgagcgag
     7801 cacgaagtgg cacagttcaa aactgcggta ctggtgttcc ttggtgccga atatgcacgt
     7861 cgcggctggg tacagcagta ccacattggc gcgctgcgta ataacaacct gcgtcagttc
     7921 aaactgctgg ggccggatgt aggctttgac tccatcaacg accgtccgat ggcagaagag
     7981 ttgtctaagc tgctgagcaa gcagaacgaa gaaaacctgc tgccgaaaac cattctgtac
     8041 tgcctgaacc cgcgcgataa cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa
     8101 ggtatgccgg gcaaaatgca gttcggttcc ggctggtggt ttaacgatca gaaagacggt
     8161 atggaacgtc agatgaccca actggcgcag ctcggtttgc tgagccgctt tgtcggtatg
     8221 ctgactgaca gccgtagctt cctgtcatac acccgtcacg aatacttccg ccgcattctg
     8281 tgccagatga tcggtcgctg ggtggaagcg ggcgaagcac cggcggacat caacctgctg
     8341 ggcgagatgg tgaaaaatat ttgctttaac aatgcgcgtg actacttcgc cattgaactg
     8401 aactaaggtc tggggttgat atgcaataca tcaagatcca tgcgctggat aacgtcgcgg
     8461 tcgctttagc ggatttggct gaaggcacag aagtcagtgt cgataaccag actgttacgc
     8521 tgcgccagga tgttgctcgt ggacataaat ttgcgttaac ggatatcgca aaaggggcca
     8581 atgtcatcaa atatggcctg ccgattggtt atgcattggc ggatattgcg gcgggggaac
     8641 acgtccacgc ccacaatacg cgcacgaatc tgagcgatct ggatcagtat cgctatcaac
     8701 ctgattttca ggatctgcct gcgcaagcgg cagatcgtga agtgcagatc tatcgtcgcg
     8761 ctaacggcga tgtcggggtg cgtaacgagc tgtggatcct gccaaccgtg ggctgtgtca
     8821 acggcatcgc gcggcaaatc cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta
     8881 ccgacggcgt gttcctcttc agccacacct acggctgctc acaactgggc gacgatcaca
     8941 tcaacacccg caccatgctg caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg
     9001 tgattggtct gggctgtgaa aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata
     9061 tcgatcctga acgcgttcat ttcatgatct gccaacagca ggatgatgag atcgaagccg
     9121 ggatcgagca tttgcatcag ctgtataacg tgatgcgcaa cgataaacgc gagccaggca
     9181 aactcagcga actgaagttt ggtctggagt gcggtggttc tgacggtctt tctggtatta
     9241 ctgctaaccc gatgctgggg cgtttctctg actacgtgat tgctaacggc ggtactaccg
     9301 tactgaccga agtgccggag atgtttggcg cagagcagtt gctgatggac cattgccgcg
     9361 acgaagcaac gtttgaaaaa ctggtgacca tggtcaacga cttcaaacag tactttattg
     9421 cccatgacca gccgatctat gaaaacccat cgccggggaa caaagcgggc ggtatcacca
     9481 cgctggaaga taaatccctt ggctgtaccc agaaagcggg ttccagcgtc gtggttgacg
     9541 tgctgcgtta cggcgagcgt ctgaaaacgc cggggctgaa cttgttaagt gcgccgggta
     9601 acgatgccgt agcgaccagc gccctggcgg gtgcgggctg ccatatggtg ctgttcagta
     9661 ctggtcgtgg cacgccatac ggcggctttg taccgacggt gaaaatcgct accaacagtg
     9721 aactggcggc gaagaaaaaa cactggatcg actttgacgc gggtcagctg atccacggta
     9781 aagcgatgcc gcagttgctg gaagaattta tcgacaccat cgttgagttt gccaacggta
     9841 aacaaacctg caacgagcgt aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa
     9901 cgctataaag tcgaaatgag ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt
     9961 ttgcttttcc atacctgata aggatttatc atgaccgcgt attggctggc ccagggcgtg
    10021 ggtgtcatcg cctttctgat tggtatcaca acatttttca atcgtgacga acgtcgcttc
    10081 aaaaagcagc tttcggtcta tagcgccgtt attggcgtac atttttttct tctgggcacc
    10141 tatcccgctg gtgccagtgc catccttaat gccattcgta cattgattac cttacgcacg
    10201 cgcagcttat gggtaatggc gatttttatt gtgctgactg gcggaattgg cctcgcgaag
    10261 ttccatcatc ctgtcgaact attgccggtt atcggtacga ttgtcagtac ctgggcgctg
    10321 ttccgctgta aagggctcac catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg
    10381 attcacaact tctgggcggg gtcgataggc ggcacaatga ttgagggtag ttttctgctt
    10441 atgaatggct tgaatatcat tcgtttctgg cggatgcaga aaaggggaat tgatccgttt
    10501 aaagtagaga aaacccctcc cgccgtagac gaaaggggtt aaacaattaa ttacgcaggg
    10561 cgctatttgc cagacgatcg tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag
    10621 ttgaagagtt cagcgcggtt tcgcaagagt cctgcaatac gccgatgata aagccgacgg
    10681 caaccacctg catggcgata tcgttcgaaa taccgaacat attacaggcc agcgggatca
    10741 gcagcagaga accccccgcc acgccggatg cgccacaggc acacagagaa gccaccacgc
    10801 tcaacagcag cgccgtgggc agatcgaccg ggatacccag cgtattaacc gcagccagcg
    10861 tcaacacggt aatggtgatt gctgcgcccg ccatattgat ggtggcaccc agcggaatag
    10921 aaacggaata ggtatcgcga tccagattca gcttttcaca cagtgccata ttcaccggaa
    10981 tgttcgccgc agagctgcgg gtgaagaagg catacacgcc gctttcgcgc aggcacagca
    11041 gcaccagcgg gaacgggtta cgacgaattt tccaccacac cagcaatggg ttaaccacca
    11101 gcgccaccag taacatacag ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg
    11161 tggagaaacc ggtggttgcc agggtagaag aaaccagacc aaaaatacca atcggtgcga
    11221 agcgaatgac cagtttcacc ataaaggtga cggcattcga catatcgttg accaggtttt
    11281 tggtggtctc gttaccgtga cgcagtgcga aaccgaggcc aattgcccac accaggatcc
    11341 cgatgtagtt acctttcagc agcgcgtcga tggggttgga aaccatgctc attaccagcc
    11401 cgcgcatcac ttctacaatg cctgacggtg gcgaaatatc acccgcgcta ctggataagt
    11461 gcagggtaga agggaaggcg aagctgaaaa cgactgcggc cagagcagca gagaaggtac
    11521 ccagcagata gaggaacaaa atagggcgga tattggtttt ctgcccgtgc tggtggttag
    11581 caatagatgc catcaccagc atcaacacca ggatgggggc aacggctttc agtgcgccga
    11641 cgaacaaagt acctaacaga ccaacagctt ccgccgcggg ttttgagatc catgccagaa
    11701 gaatccccag aacaaggccg accaggattt gttttaccag gctgccatga gccagacgcc
    11761 ggaatagccc cggtgaacgt tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac
    11821 atccctggtg cgttctgtcg acgcatcttt caggatgtaa caaagtgttt gcaaggtcga
    11881 gtataaggaa agttacagct tcgggaagcg aaaaatgctg gattttaaag agaggtcata
    11941 ttttgcaact taatggttac atttatttga cataaaaata aagccaacgt gcggcttcca
    12001 gactgatgac aacatcaaaa aaggactcgg tcagtcccct cgccccgttt ggggagaggg
    12061 ttagggtgag gggaacaggc cagcatcggt gcgaaaaatt accctcaccc cgaccctctc
    12121 cctggaaggg agagggagaa aacggtgctc atgttaatga cagggtacac ggcttaggca
    12181 gattaaaaat tatccacccc gctgcttatc atgccgataa ttcacccagg cgttgataat
    12241 aaacgtcatc accagaatgc caaacaccac gcccagcgag acggcgattg gaatatggta
    12301 gaagtcgaca atcagcatct tgataccgat aaacaccaga atcaccgcca ggccatattt
    12361 gagcatcgag aaacgctctg ctacgcccgc cagcaggaaa tacatcgcac gcaggccgag
    12421 gattgcaaac aggtttgagg tcagcacaat aaacggatca gtggtcacgg cgaagatagc
    12481 cggaatgcta tccacggcaa aaatcacgtc gctcaattcc accagaatca gtaccagcat
    12541 cagcggtgtg gcgtacagca atccattctt acgcacaaag aaatgctcgt tgtcgatggt
    12601 gtcggtcatg cgcaaatgac cgcgtagcca gcgcaccagc ggcttgtcgc caatgcctga
    12661 ttcatcttca tgggcgagcg ccatcttcac gccggtaaac agcaggaagg caccgaagat
    12721 atacaggatc cagtcgaact gtgaaatcag ccagctgcct gtgaagatca tgatggtacg
    12781 cagaacaatc gccccgagca cgccatacac cagcacgcgg cgttgtaatg ccgccggaac
    12841 agagaaatag ctgaacaaca tcagccagac aaagacgtta tcgaccgcca gcgatttctc
    12901 aatcagatag cccgtgagaa aggccagtgc ctgtggatcg gcaacggcgc gaccttcagt
    12961 ttgcaccaga taccaccaga aagcggcgtt aaacagtaac gacagcgtca cccagaccag
    13021 cgaccaggcc gcagcctgtt tcatggtcat ggcatgtgcc ccacgacgcc cctgcaacaa
    13081 caggtcgata gccagcataa tggcgacaac aacagcgaat ccgccccata gcaacggcgt
    13141 gccgacagta ttcatagaag ttccttacac ataaaaaaac ggccaacgtc ggaagacgtc
    13201 agccgctgct ttttatgcat agacctcgcc ttccggcaag gtctcactta caacaaaaaa
    13261 ggaatacgtc tgcgtattcc tttcgggttg cccggtgacc ggatgtggtt tttcacacat
    13321 cgtaatgacg atcgaccggc aatgaagtta ctcccctttg caggtaacaa agtatgacag
    13381 accattcagc cagtcaatgg tctgttacat accttttaca ctgttttact ttggcaatta
    13441 tagttttacg ctatctgccg gaaaaatcac cccagtctgg cggcggatct cggtcagcag
    13501 tttggcggtg atgcgactga ccgccagccc cggatgatcc accagatgct catccaccag
    13561 ggtagcgaac agctctgctt cgtagagcat ggtattaata tgctgcggct gggtgagatc
    13621 ctgcatttgg ctgccacgcg gcacgaagca cactttctgg cattcagaca gtttttcaat
    13681 caccagcgat cctgcttcgc cctgaatctc gctcgccagg acagaatcac tgacttttga
    13741 gtgctgcaag gtgacgctga aatcaccgta atccatcacc accacgccgt gggcatctac
    13801 accgcttgcc agcaaactgg cggttgcctg cacgcttttc ggctcaccaa ataacgccac
    13861 cgccgacgcc aggcagtaaa agccgatatc cataattgaa ccgttagaga atgacggatt
    13921 aaaggtgttg ggattttcac cgtccaggta acgttgatag cgcgaggaat actggcaata
    13981 gttgaaaaag actttacgca atttgccgac tttcggcagc gcctggcgca gcaaatgaaa
    14041 gttcggcagg caggcggttt taaatgcctc aaacagcacc acctgatttt cccgcgcaca
    14101 ggcaatggcg gcatccactt ccgccagatt cgacgccagt ggtttctcgc aaatcacatg
    14161 aattttatgg ctaaggaaaa gttgtgtctg ggaaaagtgc agggcattcg ggctggcaat
    14221 atacaccgcg tcaatggcat cgctttccgc catcgcttcc agcgaggtaa acagatgctc
    14281 gacagaaaaa tcattggcga agtgctgagc ctgctcaagg ctgcgggaat atacggcggt
    14341 taacttgtat ttaccgctct catgggcggc ctcgacgaac tggcgagtga tccagttcgt
    14401 accaatcaca gcgaaacgta tcataacgcg tggatactcc ataaagggat tctgtcgtca
    14461 atgtagcacg cctttttcat cttcgacgtg cgcagatacg cattattctt ttgccggaca
    14521 gccatgctca ctaaacggcc caatcatgac cgatggcccg agatatttcg gctggcgatg
    14581 gaggattttg gcgtcaatca cggcgctgac acgagcgagt ttctcccgca gttgagtata
    14641 aaaaccgtgc ttcatattga ggtcaggcgc gttaaaaccg atagcgtcga tgccgtactg
    14701 ttttgccagc cagatggcgc gttgattatg aaattcttgc gagataatgg tgatatggtt
    14761 ttcaccaaaa acctttttgg ctcgcactac cgaatccagc gttgagaatc cggcatagtc
    14821 gcagaagatc actttcgccg gtacgccttt agcgatcaag cctgcatacc tgaggcttca
    14881 tcgtaatttt tacgtccgtt atcgccactt accagtagcc atttcacttt tccggcgtgg
    14941 tacaacgctg ctgcggtatc aatacgtcgg gtaaagtagc gattacccgg cctggccccc
    15001 agcaataagc ccacgttacg cgccggaact gcgttgacat cgctccaggt cagctgcttg
    15061 ctggcgttga ccataacgcg atcggcaata aaaatggtcg ctcctgctat aagcaacaac
    15121 aaacagccaa tcttaagagt ccggcgagaa aaacaaatac ggagaagaag gcggggctgc
    15181 atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag aaagaaaaga
    15241 taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg ttagccacag
    15301 ccgggtgtcg aactcaagct ggtggtactg cggctccata tggcagcaca actggtaaaa
    15361 cgctttgtta tgctctttct ccttcaggtg cgccagttcg tgcaccacga tcatacgtaa
    15421 aaacggttcc ggcgcgttgc gaaacacagt agcgacgcgg atctctgctt ttgctttcag
    15481 cttgccgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt ttagcacatg
    15541 gatcttattg tcatacatca ctttattgat cggcggggca ttgcgcagaa actgattttt
    15601 tagatccgga gtatattgcc agagggcttt atcggtggcg tagtcgtgtg ttcccggata
    15661 gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agcgttcgca cctgggaaag
    15721 tagctgctcg ggataaccct gaagataagt aagattgctc ataaccgccc acgaaaaaga
    15781 gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta tcattcagga
    15841 gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg ttttccggcg
    15901 acggatgacg ttaacccgct acaggcgtgg gaagcggcgg atgaatattt gctgcaacag
    15961 ttggacgaca cagaaatccg cggcccggtg ttgatcctga atgatgcctt tggtgcgtta
    16021 agctgcgcgc tggcggaaca taagccgtac agcattagcg actcatacat cagtgaactg
    16081 gcgacgcgcg agaatttacg cctcaacggg attgatgagt cgagcgtgaa gtttctcgac
    16141 agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc gaaaacactg
    16201 gcattgctgg aacaacaact gcgtgcgctg cgcaaagtgg tcacgtcgga tacacgtatt
    16261 attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt cgaaaaagtg
    16321 ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa ttgcacgttc
    16381 aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga aggtactgac
    16441 tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat cggcgcgcgc
    16501 ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct cggttgcggt
    16561 aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt ggtgtttgtc
    16621 gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa catgccagag
    16681 gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga gcctttccgc
    16741 tttaatgctg tgctctgcaa cccgccgttt caccaacaac atgcgctgac cgataacgtc
    16801 gcctgggaga tgttccacca tgcccgccgc tgcctgaaaa tcaacggcga gctgtatatt
    16861 gttgccaacc gtcacctgga ttacttccat aaactgaaga agattttcgg caactgcacc
    16921 accatcgcca cgaataataa atttgtggtg ctgaaagcag tgaagctggg gcgtcgtcgg
    16981 taagattggg agttacctgc cggatgcggc gttaacgcct tatccggcct acgttcagca
    17041 cgttactccc aggccggata agacgcggca agcgtcgcat ccggcaatgt ctgcccagtt
    17101 ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc atcctctaca ttctcttgtt
    17161 tagcgttgtt caacgtttat gctacatacg tgaagttatg acgcataaag aatacgattt
    17221 ctttaccgct gttcatcgta ctaaggggaa aaaatgattg cgattgccga catcttgcaa
    17281 gcaggagaaa agctaactgc tgtggcacct tttctggcgg gtattcagaa cgaggaacaa
    17341 tacacccagg cgctggaact ggtagatcat ctgctgctca acgatcctga aaaccccttg
    17401 ctggatctgg tgtgtgcaaa aataaccgcg tgggaagaat cagcgcccga atttgcggaa
    17461 tttaatgcca tggctcaagc catgcctggc ggtatagccg tgattcgtac ccttatggat
    17521 caatatggtt taaccctttc cgatctgccg gaaattggca gtaaatctat ggtgtcacgc
    17581 gttttgagcg ggaagaggaa attaacgctg gaacacgcta aaaaattggc aacgcgattc
    17641 ggcatttctc ccgccttgtt tattga
//
LOCUS       DAZGLJ010000091        17655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000091 DAZGLJ010000000
VERSION     DAZGLJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.091"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(67..1068)
                     /locus_tag="ACHK49_002616"
     CDS             complement(67..1068)
                     /locus_tag="ACHK49_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO9263242.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1175..1471
                     /locus_tag="ACHK49_002617"
     CDS             1175..1471
                     /locus_tag="ACHK49_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO9263243.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1605..2030)
                     /gene="arsC"
                     /locus_tag="ACHK49_002618"
     CDS             complement(1605..2030)
                     /gene="arsC"
                     /locus_tag="ACHK49_002618"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO9263244.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(2043..3332)
                     /gene="arsB"
                     /locus_tag="ACHK49_002619"
     CDS             complement(2043..3332)
                     /gene="arsB"
                     /locus_tag="ACHK49_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO9263245.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3386..3739)
                     /gene="arsR"
                     /locus_tag="ACHK49_002620"
     CDS             complement(3386..3739)
                     /gene="arsR"
                     /locus_tag="ACHK49_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO9263246.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            4479..4562
                     /gene="dinQ"
                     /locus_tag="ACHK49_002621"
     CDS             4479..4562
                     /gene="dinQ"
                     /locus_tag="ACHK49_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO9263247.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4616..5968)
                     /gene="gorA"
                     /locus_tag="ACHK49_002622"
     CDS             complement(4616..5968)
                     /gene="gorA"
                     /locus_tag="ACHK49_002622"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO9263248.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6040..6882)
                     /gene="rlmJ"
                     /locus_tag="ACHK49_002623"
     CDS             complement(6040..6882)
                     /gene="rlmJ"
                     /locus_tag="ACHK49_002623"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO9263249.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            7085..9127
                     /gene="prlC"
                     /locus_tag="ACHK49_002624"
     CDS             7085..9127
                     /gene="prlC"
                     /locus_tag="ACHK49_002624"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO9263250.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHEEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            9135..9887
                     /gene="rsmJ"
                     /locus_tag="ACHK49_002625"
     CDS             9135..9887
                     /gene="rsmJ"
                     /locus_tag="ACHK49_002625"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO9263251.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9936..11405)
                     /gene="dtpB"
                     /locus_tag="ACHK49_002626"
     CDS             complement(9936..11405)
                     /gene="dtpB"
                     /locus_tag="ACHK49_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO9263252.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            11571..11624
                     /locus_tag="ACHK49_002627"
     CDS             11571..11624
                     /locus_tag="ACHK49_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263253.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(11723..12157)
                     /gene="uspA"
                     /locus_tag="ACHK49_002628"
     CDS             complement(11723..12157)
                     /gene="uspA"
                     /locus_tag="ACHK49_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO9263254.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12548..12883
                     /gene="uspB"
                     /locus_tag="ACHK49_002629"
     CDS             12548..12883
                     /gene="uspB"
                     /locus_tag="ACHK49_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO9263255.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(13128..14627)
                     /gene="pitA"
                     /locus_tag="ACHK49_002630"
     CDS             complement(13128..14627)
                     /gene="pitA"
                     /locus_tag="ACHK49_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO9263256.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14859..16061
                     /gene="yhiN"
                     /locus_tag="ACHK49_002631"
     CDS             14859..16061
                     /gene="yhiN"
                     /locus_tag="ACHK49_002631"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO9263257.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(16377..17429)
                     /gene="yhiM"
                     /locus_tag="ACHK49_002632"
     CDS             complement(16377..17429)
                     /gene="yhiM"
                     /locus_tag="ACHK49_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO9263258.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4595 a         4456 c         4524 g         4080 t
ORIGIN      
        1 tatgattttc tacttagaag gatatggcac taactatgca tcttatagct tactgctggc
       61 atctatttaa aatagcaatt gagtcaaata gcccatcaag atagccatac ctaatataac
      121 cgtcaggaat gcagctatca tcggcattct gaacattgat ttaagtaata tcacttccgt
      181 caggcttgcg ccggcactgc cgatgattaa tgccattaat gctcccagac ccattccttt
      241 tgtcatcaga acagatgcca gagggataac tgcctcagcc cggatataca gcggaatacc
      301 gacaacggcg cttaatggga tggcgaaggg attatctgct cctgcatgag cggcaatcca
      361 cgctgaggga ataaagccat aaataaaaga tcctattaat actcctaaca aaagatatgg
      421 cagtacatct ttaaactgtt gcaacgcatc tttggtggct agttttatgg gattaatact
      481 tactacagct ttggtactgc aacaggtaac tacggggttc tcaatggctt tagccgctgg
      541 actacaacaa ctcactttta tgggcgtata tgtcgttccc ggcgaagttt ttgctggagc
      601 acaacaattt gctgatgagc ttttactggc aatgatatga cgttcaaacc ccagagaatc
      661 caggataata ctggcaagaa cggagacgcc ggctgcgata atcgcgtaca acaaggtgac
      721 tttccaacca aaggtcatcc acattagtcc gacgataatg ggattaagta atggtgaaac
      781 aaataaaaaa gtgagagtcg gaccaaaccc ggctttcgct gacaacaatc cacgtaacat
      841 gggtattgtc gagcaactac agaacggggt aacggctccc aacagagcag ccaggagata
      901 gcctctccct tttctggccc ccatcatctg ctggatttta tggtctggca ccttttgtct
      961 tatcaggctg acaccggcac taatcactat aaacagcaac gaaagctcga cggcgagaaa
     1021 cacaaacatc tctgcggcat cttgcagcat cgcaagccaa ctactcatac gtaacctcat
     1081 tggataaatc tatatttcca gaataatcga aatgaaattc agatcaagat atttctggta
     1141 ttatcgaaat atcaaccaga cagaggggtg tcatatgcag ttggaagaag tcgctaaagc
     1201 gctgaaagag ctaggtcacc cgacccgttt gttcatattt aaacatcttg tcaaagccgg
     1261 ggaacaaggg ttgccggtcg gtgagttgca aaagcagctt gggatacctg gctccacact
     1321 cagccaccat atatcagcac tggtatcggt aggactggtc aaacaaaacc gtgagagtcg
     1381 gacgctgatg tgtgtttcgc aatacgcaat gctggaagcg ataattgaat tcttacgcga
     1441 ggagtgctgc gtaaatagca aaattgcctg atgcgctacg ctctacatat ttcatgccat
     1501 atattgaatt tgcacggttt tgtaggtcgg ataagacgcg ttagcgtcgc atccgacata
     1561 aacaaagcgc actttaccaa ctatcggcag tggatatcgc cgctttattt caggcgctta
     1621 cccgcttcat caaccacttt ctcgccatct tccttggaga atgcgccttt ttgcgcatct
     1681 ggcagaattt ccagcaccac ttctgaaggg cggcacaggc gagttcccag cggcgtcacc
     1741 acaatcgggc gattaatcag aatcgggtgc tgaagcataa agtcgattaa ccgatcgtca
     1801 gtaaatttat cttccgcaag gcccagctcc tcatacggtt cgacgttttt acgcagcagc
     1861 gcgcgtacgg aaatccccat atcggcaatg agtttgacca gttcatcgcg cgttggcgga
     1921 gtttccagat aatggataat ggtcggttct gtgccgctgt tgcggatcat ctccagcgta
     1981 ttacgcgacg tgccgcaggc cgggttgtga taaatggtaa tgttgctcat atcagtatct
     2041 cattacaaag tgaaagagag acgtagcgcc agcgcagcca gcgtcacaaa cagcacaggc
     2101 agggtcatga taatccctgt acggaagtaa tatccccagc tgatagtcat attcttctgc
     2161 gaaagtacgt gcagccagag tagcgtagcc aggctaccaa ttggggtaat tttcggtccc
     2221 aaatcgcagc caatcacatt ggcataaacc atcgcttctt tgataacgcc agatgccgtg
     2281 ctgccatcaa tggacaacgc gccaaccagt accgtcggca tattgttcat aatagaagag
     2341 aggaaggcgg tgaggaatcc ggtgccgagc gtcgcggccc acaggccgtt atccgccagc
     2401 acgttgagta cgccagaaag atattccgtt aatccggcat tgcgcaggcc ataaaccacc
     2461 agatacatgc cgagcgagaa gatgacaatc tgccaggggg caccgcgcag gactttaccc
     2521 gtattaatcg catgaccgcg tttagcgacg acaaataata tcagcgcgcc cacagctgca
     2581 atggcgctca ccggaatgcc gagcggttcc aggacgaaaa atcccaccag cagaagcagt
     2641 aaaacaaccc agccagtttt gaacgtagca ggatctttga tcgcttctgc gggagatttc
     2701 agcagcgcca tatcgtagtt ctgcggaata tctttgcgaa aatagagatg taacatcacc
     2761 agcgtggcaa caatcgcggc gatatccacc ggcaccatca ccgaggcgta ttcgcgaaag
     2821 ccgaggccaa agaaatcagc ggaaacgata ttcaccaggt tggagacaat aagcggcagg
     2881 ctggcggtat cggcaatgaa tccggccgcc atcacgaacg ccagcgtagt gcctttactg
     2941 aaccctaaag ccagcagcat ggcgatgaca atcggtgtca aaataagcgc cgcgccatca
     3001 ttggcaaaca gggcggcaac ggcagcaccg agcaggacaa tccaggtaaa cagcaagcga
     3061 ccacgaccat taccccagcg tgagacgtgc agcgccgccc attcaaaaaa gccggactca
     3121 tccagcagca ggctgatgat aatgacggcg ataaacgcag ccgtcgcgtt ccagacgata
     3181 ttccacacca ccggaatatc acccggatgg accacgcccg taactaacgc cagtactgcg
     3241 ccgagcgttg cactccagcc gatgcctaaa cctttcggct gccagataac caatacgatg
     3301 gtcaggacaa agatagcgcc tgccagtaac ataatgcctc ccggataaaa cacatctgaa
     3361 aattcatatg tgtttagcta aatttttaac tgcaaatgtt cttactgtcc ccggaacagt
     3421 tttgtcgagc caggttgcgg acaatcgcct gaaccttttc ctgttcacat cgccaggcct
     3481 catcaataat tttcgccgcc catgctggaa tatgcggtga taagcggtaa tgaacccact
     3541 taccttgctt gcggtccagc aatagcccgc tttcacgcag caatgccagg tggcgggaga
     3601 tcttgggctg cgactggtcg agagcagtgc agagatcgca gacgcataac tctcccagtt
     3661 cgctgagcag taaaacgatg cccagacggg tttcatcagc aagaattttg aacaattgga
     3721 tgggtaacag aaatgacata ttgcgctcct gattgttgca ggtagtgtct ctcttcgaag
     3781 cggataagtc aaaaacatat atgacttaac gaatgtgtaa gtgcagagga aggtaatagg
     3841 tgtgaatttt gagttggcta ttcatttgaa aggaggtctg aatcaggacg taaaaaaacc
     3901 gactttgcgt cggtttttta ctttccagcc ctgagttggt ggctctggct ggagtgagag
     3961 agttcttatc taacagctca atactattag ccagtggcta acattgcaag aattagcgat
     4021 ttcttcagct ggcggagggg gcacttcggt atatatcgca atgacgattt atatactttc
     4081 actgggtcat cgtcatatta agccttgtcc gaatccggat gtaaaaaaac cgactttgcg
     4141 tcggtttttt actttccagc cctgagttgg tggcactggc tggagtgaga atgccattat
     4201 ctaacgcatc aatgctaata ccatactgaa actattgcaa ggacatgctg gttttataac
     4261 ctgaatgtac tgtatgatta tccagttagc tctgaggcat tttcactctg gtaatgcgca
     4321 taaacgcttt caaagtcctg gtcagaagta cgggtggtgc cgttaactga tgctctggcc
     4381 ggagtgagaa tgccattatc taacaatagg gcatgcgccg tgacaggcag tggatgagta
     4441 agcggatgca ttctcactcc atcgcatgga gaaaacgggt gattgataaa gcaatcatcg
     4501 ttcttggggc gttaattgcg ctgctggaac tgatccgctt tctgcttcag cttctgaact
     4561 gatagcggaa acgtaattaa gggctaagag cacactactc ttagcccttt aacatttaac
     4621 gcattgtcac gaactcttct gccgccgttg ggtgaatggc gacggtattg tcgaagtctt
     4681 ttttggttgc acccatcttc agcgccaccg cgaagccctg caacatttcg tccataccaa
     4741 agccaatgcc gtgaataccg acaatcttct cttccgatcc aacgcacacc agcttcatgc
     4801 ggcacggctg gcggtgagtg gtgacggcgg tatacatcgc ggtgaaagag gatttataca
     4861 ctttcacctg atcgtcgcca tactgctcgc gcgcctgcgg ttccgttaaa ccaacagtac
     4921 caatcggcgg atggctgaag accacggtcg gaatgttgct gtaatccaga tgctcatccg
     4981 gcttgttatt aaacaggcgt tcagagagac ggcgacccgc tgcaactgcc accggtgtca
     5041 gctccactgc acccgtgtta tcgcccaccg cgtaaatacc ttcaatattg gtgttttgat
     5101 atttatcgac gacgatatag cctttttcgt tagttttaac gccagcggct tccaggttga
     5161 tgttgtcatt ggcaggctcg cgaccaatcg cccaaatcag gcaatccacc gtttcactgc
     5221 gaccatcttc cagctccagc gtcaggctac catcggcatt tttcactacc gctttcggga
     5281 tggcgttggt gtgcagctgc gggccttcgg cgttcatcac ttcgaccagc gtttcggaaa
     5341 tcatcgggtc gaagctgcgc agcggcgcat gtttacgcac aaacagatgc gttttcgcgc
     5401 cgaggccgtt aatcacgccc gccagctcaa cggcgatgta acccgcgcca acaaccgcca
     5461 cgcgctctgg caaagcagga agggcgaaga agccatcaga atcaataccg tattccacgc
     5521 ccggaatatc cgggtggctc ggacgaccgc ctgtggcgat cagaatatga tcggccgtga
     5581 tggtttcgcc gtttacctcc agcgttttgg catcaacgaa gcgggcaaag cctttgatta
     5641 catcaacgtt atttttaccg agcacgtttt cataggaagt atgaatacgg tcgatatagg
     5701 cggtacggct ggcgatcaac gtttcccagt tgaatttatt gatagtggta tcaaaaccat
     5761 aatccgggcc gtacatatgg atcgcttcac ggatttgcgc cgcgtgccac atcacttttt
     5821 tcggcacaca gccaacattt acgcaggtgc cgcccagctc tttggcttca atcagcgcac
     5881 atttctggcc gtacatagcc gcgcggttga tggaggcgat accgccgctg ccgccgccga
     5941 tggcgatgta atcatagtgt ttagtcatga caaagtgtcc ttatcgttga ttaccgcgat
     6001 tgtagcgcga gacgtaatag gtgccagcaa tggctgcaat tactccggca cgatccagct
     6061 tacggtggcg tgcccggtgc ctgccggaac cagtttgctg tgcagccacg gcagcacgtt
     6121 attcatctgc tgttccagtt tccacggcgg gttaatcaca atcatgccgg aagcggtcat
     6181 gccacggcga tcgctgtctg gcagtaccgc cagttcaatt tgcagaattt tgcgaatacc
     6241 ggtcgcttcc agatcgtgga tcatgcgctt aatttgctga cgcagcacca ccggatacca
     6301 cagtgcgtaa gtaccagtgg cgaaacgttt gtaaccttct gctatcccgc tgaccaccgc
     6361 ctgatagtcg gttttcattt catacggcgg gtcgataagg attaaaccac ggcgggaaac
     6421 cggcggcagt ttggccttaa gctgctggaa accatcggct ttttcgacgc gcgcacggct
     6481 gtctttctga aattcagaac gcagcaacgg gtaatcgctc gggtgcagtt cggtcagttg
     6541 caggctgtct tgttcgcgca gtagctggcg agcaatcaat ggcgaaccgg ggtagtaacg
     6601 caactgaccg ctacggttga agtgttttac cacattgatg tacgcctcca gttctgcggg
     6661 caaatcgtcc tgctgccaga tacgggcgat gccttcgaga tattcgccgg tacgctcggc
     6721 atgttcgctg cctaactgat aacgcccggc ccctgcgtgg gtgtcgagat agagaaacgg
     6781 tttatctttc tctttcagcg actcgatgat caggctctga acggtatgtt taaggacgtc
     6841 ggcgtggttg ccagcgtgaa agctgtggcg ataactgagc atgggtaaag gtgttccggt
     6901 aagtaaaaat cggcccgtat tcggggcgca caaaagcgta tcaggacagt ataccgaaaa
     6961 gaggccgccg ccgcgaaagc gtaacgtttc tcattgaaat tcactacact taaccccatg
     7021 ctacacacat tatgtaaagc gcctgttgag cgcttcctta acctctttaa ccaggactgc
     7081 gcgaatgacg aatccgttac tgactccctt tgaattgcct ccgttttcta aaattctccc
     7141 ggaacatgtc gttccagccg tgactaaggc gctgaacgac tgccgcgaaa atgtggagcg
     7201 cgtagtagcg caaggagcac cgtacacctg ggaaaatctc tgccagccgt tggcggaagt
     7261 ggacgatgtg ctggggcgta tcttctcccc ggtcagccac ctgaactcgg tgaaaaatag
     7321 cccggaactg cgtgaagcgt acgaacaaac cctgccgctg ctgtcggaat acagcacctg
     7381 ggtagggcaa catgaagggc tgtataaagc gtatcgcgac ctgcgcgatg gcgatcatta
     7441 cgccacgctg aacacggctc agaaaaaagc ggttgataac gcactgcgcg acttcgaact
     7501 ctctggcatc ggtctgccaa tagagaaaca acaacgctac ggtgagattg ccactcgtct
     7561 ttccgagctg ggcaaccagt acagcaacaa cgtcctcgat gcgacgatgg gctggaccaa
     7621 actcgttacc gacgaagcgg agctggcggg gatgcctgaa agcgcgctgg ctgcggcaaa
     7681 agcccaggcc gaagcgaaag agctggaagg ctacctgctg acgctggata tcccaagcta
     7741 cctgccggta atgacctact gcgacaacca ggccttgcgt gaagagatgt atcgcgctta
     7801 cagcacccgc gcctccgatc aaggcccgaa cgccggtaaa tgggataaca gcaaggtgat
     7861 ggaagagatc ctcgctctgc gtcacgaact ggcgcaactg ctgggctttg aaaactacgc
     7921 ctttaaatcc cttgccacta aaatggcaga aaacccgcag caggtgctgg atttcttaac
     7981 cgatctggca aaacgcgcgc gtccgcaagg cgaaaaagag ctggcacaac tgcgcgcctt
     8041 cgccaaagcg gaatttggcg tcgatgagtt gcagccgtgg gatatcgcgt actacagcga
     8101 aaaacaaaaa cagcacctct acagcatcag cgacgagcag ctgcgtccgt acttcccaga
     8161 aaacaaagcg gttaacggcc tgtttgaagt ggtgaaacgt atttacggca tcaccgctaa
     8221 agagcgtaaa gatgttgatg tctggcatcc ggatgtacgt ttcttcgaac tgtatgacga
     8281 aaataacgaa ctgcgcggca gcttctacct cgatctgtat gcccgtgaaa acaagcgcgg
     8341 cggggcgtgg atggatgact gcgtaggcca gatgcgtaaa gctgatggtt cgctgcaaaa
     8401 accggtcgcg tatttgactt gtaacttcaa ccgcccggta aatggtaaac cggcgctgtt
     8461 cactcacgag gaagtgatca ccctgttcca cgagttcggt cacggcctgc accatatgct
     8521 gacccgcatc gaaaccgctg gtgtttccgg tatcagcggt gtgccgtggg atgcggtcga
     8581 actgccgagt cagtttatgg aaaactggtg ctgggagccg gaggcgctgg cgtttatctc
     8641 cggtcactat gaaaccggcg aaccgctgcc gaaagagttg ctggataaaa tgctggcggc
     8701 gaagaactac caggcggcgc tgtttattct gcgtcagctg gagttcggcc tgtttgattt
     8761 ccgccttcat gccgagttcc gcccggatca gggggcaaaa atcctcgaaa ctctggcaga
     8821 aatcaagaaa ctggttgccg tggtgccatc tccgtcctgg ggccgtttcc cgcacgcttt
     8881 cagccatatt ttcgccggtg gttatgcggc aggttactac agctacctgt gggctgacgt
     8941 actggcggca gatgctttct cgcgctttga ggaagagggc attttcaacc gtgaaaccgg
     9001 gcagtcgttc ctcgacaaca ttctgagccg tggcggttca gaagagccga tggatctgtt
     9061 caaacgcttc cgtggtcgtg aaccgcagct ggatgcgatg ctggagcatt acggcattaa
     9121 gggctgatca ttcagtgaaa atctgcttaa ttgatgaaac aggcgccgga gacggcgcct
     9181 tatctgttct ggcggcccgc tgggggctgg agcacgatga agacaacctg atggcgctgg
     9241 tgttaacgcc ggaacatctg gaattgcgca agcgtgatga gccaaaactt ggcggcatat
     9301 ttgttgattt tgttggcgga gcgatggcgc accgacgcaa attcggcggt ggtcgcggtg
     9361 aggcggtggc gaaagcggtg ggcattaaag gcgattattt gccggatgtg gtggacgcca
     9421 ccgcagggct ggggcgcgat gcctttgtgc tggcttcagt cggttgccgc gtgcggatgc
     9481 tggagcgtaa tccagtcgtt gccgcattgc tcgacgacgg cctggcgcgt ggttatgcgg
     9541 atgcggaaat cggcggctgg ttgcaggagc gtttgcagtt aattcacgcc tccagcctga
     9601 cggcgctaac cgatattacc ccacgcccgc aggtggtcta tctcgacccg atgttcccgc
     9661 ataagcagaa aagcgcgctg gtgaaaaaag agatgcgtgt ctttcagtcg ctggtggggc
     9721 cggatcttga tgccgatgga ttgctggagc ctgcacgctt gctggcgacc aaacgcgtgg
     9781 tagtaaagcg cccggactac gcgccaccgc tagcgaatgt cgccacgcca aacgcggttg
     9841 tgactaaagg gcatcgcttt gatatctatg caggtacgcc cgtgtaaaag taaagcaggg
     9901 tagcgataac gctaccctgc tttctgcaag aataattaat ggctttccgg cgtcgcaatc
     9961 atgcgtttca gccacggcac catcaacagc atcactactg caacgcccag cgtgaccaga
    10021 ccaatcttac caaacacgtt ggtatagacg ggcaacgtct caagcggatc ggtaatgttg
    10081 tccggcaccg cggtaaatgt tgccacatag ccgcccagca agaacgcggc agcctgcgtc
    10141 aggaaccaca tcccgagaat aaagcccatc aaatgctgcg gcaccagggc agcaatcatc
    10201 gccaggccaa gggcgctaat aaacagttca cctaagctct ggaataagta caccagcacg
    10261 ataaaccatg gcgatgtcag cccttgtgca tccgcaaacc acattcccgc agctgccgcc
    10321 gtcaaaaagc ccagtgagca cataaacatg ccgagagtaa atttcatcgg catcgagagg
    10381 tctttgcctt tgttacccag atgcgtgtaa atgcctgcca gtattgggct ggcgagtacc
    10441 acccagaacg ggttaagcgc ctggaagctg accgggttga tggaaaaacc gagaatttca
    10501 tgatgcacgt tgttgatggc aaagaagttc agcgatgttg gcatctgggc gtagagaatg
    10561 taaaacacca ccgcttcgag catcaggaca aaggcgacaa acattttatt gcgcccggtt
    10621 ttatccagct tgaatgcctg acgaaagaag atgatggtga cgacgatgga gagaacaatc
    10681 agcaccagat tggcgacttc tacgttgtgc atcagccatg cacatacgaa gatcatcacc
    10741 acgctgccaa gtaatacgta caacagtttg ctgaagctca tcggcttgaa gtcgggttca
    10801 gaaccaatgt ctttcaccat tccacgacag gcgatgtaaa ccagtaatgc gataattaac
    10861 cccgccccgc acaggttgta ggtgactgaa taaccgaatc tatcagcgat cacaggggcc
    10921 agcgataacg ctatcaacga gccgatattg atcgacatat agaacagggt gaatgcgcca
    10981 tcaagccgcg gatctttcgg cggatagcac ttcgaaagca agctggctgg gttagcttta
    11041 aacaggccgt taccgacagc gatagtcccc agggcgatga aaatcaggtc aggcttaagt
    11101 agcgacatgc cggtcatgaa gtagccaatc gccagcacaa gtgctccgag aacaatggtg
    11161 cgtttggtcc ccagcaggtg gtcgccgaca tagccgccaa tggaaatgag gccatagacc
    11221 agcgcagcaa aagcaccaaa agtgacaaaa gcctgctctt gcgagaatcc aagctgttta
    11281 acgaagaaaa ccgccagtac gccctgcacg ccgtagtagc cgaatcgctc ccataactcg
    11341 acaaaaaaga tcatgaaaaa tgggcgaggt tgctgcagca tccccatggg tgttgttgta
    11401 ttcatattta ttaaccttcc aataccatcc tgaaaagtat gaacgcgtaa gggataacgc
    11461 gcgaaaaagt ttacatctgt cagagattct taatattgat gcggtgaata gattaataca
    11521 ctattgcaac agaaaataac cagctgatca cactaatgat ggaaaatatg atggtgttaa
    11581 atgttgtatt ggcaggagat tttgattgcg tacgcagatc gtaggccaga taaggggttt
    11641 acgctgcatc aggtaacaag gcgcgccaaa aagcaaaacg cccgcatatg cgggcgttca
    11701 ggaacacgtc gtggagggat gattattctt cttcgtcgcg cagcggaaca atcagcatat
    11761 caacgtgaac ggtgttgatc agctgacgtg cggaagacat cagtttgctc cagaagtcct
    11821 ggtggtgacc acaaaccacc agatccatat cgtatttctt gattgcatcg accagaacct
    11881 ggcccaggtc gccgctgccg ctcagggttt cagtgattgg gtagcctgca ttagtggaaa
    11941 gctcggtcag cgcatgatgt gtctcttcag agatgcgttt ctgcatatca cccagattca
    12001 catcaataag cccggtgtat aggtcagagt agtttacatc tacgtggatc agagaaactt
    12061 tcgcattgta ggggcgagcc atagagactg ctttctctac cagaactttg ctttccgggg
    12121 agagatcgac tgcgatgaga atgtgtttat aagccatagt gttactcctt ccataaagtt
    12181 gtcgatgact ggccagctag cgtttcttgt gccgttacgt cacccgcgtc ctgcgaacta
    12241 cgtgcgcggg gctaacccag ccgtaaagat tcagtgttaa gaccatcctt accttatagc
    12301 gacccggatg atccgtcaat ccgccttgct tacccgttag ataaacaaat tttcgacatg
    12361 aatatattga tagtggttaa ccttctggaa aaaaaacaac ctgatctcct acactatcta
    12421 tagagccgct cggatgttgg tcaccacaaa agcggtttct ccaggacctg gtgttaactg
    12481 attggcagcg tatcgggagc ggtagccccg gggtggaact ccgcgggcag gtcgccgggg
    12541 aggaggtatg ataagcaccg tcgcattatt ttgggcttta tgtgtcgttt gcattgttaa
    12601 catggcgcgc tatttctcat cactacgcgc gttgttagtg gtactgcgta actgcgatcc
    12661 attgctctat caatatgttg atggaggggg cttttttacc tcacatggcc aacccaacaa
    12721 acaggtgcgt ctcgtttggt atatctatgc ccaacgttat cgcgatcatc acgatgatga
    12781 atttattcgc cgctgtgagc gggtgcgtcg gcagtttatt ctgaccagcg cattgtgtgg
    12841 tctggtggtg gtcagcctga ttgcattgat gatttggcat taagcacttc agcaataata
    12901 acgcataaaa aaagcgggcc agtaaactga cccgctcgag actgattaca aacgcaaaat
    12961 tgcctgatgc gctacgctta tcaggatcta cataatctct gcaatctatt gaatttgcat
    13021 gtttttgtag gccggataag gcgttcacgc cgcatccggc atgaacaaag cgcactatgt
    13081 cacaatctga agcgggccag ctgacccgct cgttttggtg tgtacgatta caggaactgc
    13141 aaggagagcc agtacagtcc gccggaaagc agtaccgcag ccggaagggt aaacacccag
    13201 gccatcagaa tactggttac ggttttacgc tgtaagccgc cgccatctac caccatcgtc
    13261 cccgcgacag aagaggagag tacgtgagta gtggaaaccg gcatcccggt ataactcgcc
    13321 aggccgatag acactgccgc cgtcatctgg gccgacatcc cctgagcgta ggtcatgcct
    13381 ttcttaccga ttttctcacc gatagtcgtt gccacacggc gccagccaat catcgtaccg
    13441 atacctaacg ccagcgcgac cgccatgatg atccacaccg gtgcatactc gatggtgcta
    13501 agcatgtcgg acttcagttt cttcaacagg cgctgatcgt cagcactcac accaggcatc
    13561 ttcaccactt tgtcgatagt gtcagaaacg cacagcataa tgcggcgcat ctggctacgt
    13621 tgatcaagcg acagcttgtc gtagctttcc acatcggtag tcaacatgcc tttcaggcgg
    13681 ttgagcgcgt taatggtatt cgacggatgg caatggaact ccgcgggttg cgttgcgcca
    13741 gcttccggag ccggtactaa ctgatcagca ccggtagcct gtttgagcag cgcaggatgc
    13801 tgctcaaagt aagcttcgac gttgttgatg gcatcacggg tacgggtgat ttcgtagcca
    13861 gtggcattca tgttcaccac gaagcctgct ggtgcgacgc caatcaatac caacataacc
    13921 agaccaatgc ctttctgacc atcgttcgcg ccgtgcgaaa acgccacgcc gatagcggaa
    13981 aggatcagcg caatacgcgt ccagaacggc ggctttttct tgccgtcttt cttttcacgc
    14041 tccgctgggg tcaggtggat acgggcgcgt ttcttggtgc cgctccagta gcgacgcagc
    14101 aagaaaatca gaccgccagc aaacaccagg ccgacaatag gggaaacgat cagagaaccg
    14161 aaaatactta atactttcgg gatattgagt gcatccacca ctgacgtccc ggtcatcaac
    14221 gcattggtta aaccaatccc gatgatcgcg ccaatcagcg tatgagagct ggatgcaggt
    14281 aaaccaaagt accaggtacc caggttccag ataatcgccg ccagcaacat agagaacacc
    14341 atggcaaggc catgagacga tcccatatta agcagcagat ccgtcggcag catatgcaca
    14401 atggcatagg caacactcag accacccagc aaaacaccca aaaagttgaa caccgctgcc
    14461 ataaccacgg cgagctgaga acgcatcgcg cgggtataga taacggttgc cacggcgttg
    14521 gctgtgtcat ggaaaccatt gatggcttcg tagaacagca caaaagccag tgcaagcaat
    14581 aataacagcc cggtatgcaa atccaggcca gcaaacaaat gtagcatagg acgttacgcc
    14641 attttgagga catgaacgcg gcgcattatc agtgactttc gcggcgcggg caaagtgaaa
    14701 tatagacttt ttttgatttg cctcctgtat ggatttcact caaaaaataa ttatcttata
    14761 taattcaggc aaatacttcc ttttagtaat attgatgctg gtgcgaccac tgaggaatct
    14821 ttacaattca cgcccgtttt ttctaagagg agcgcaacgt ggaaaggttt gatgccatta
    14881 ttataggcgc tggtgcggcg ggtatgttct gttctgcgct ggcaggtcag gcaggacgcc
    14941 gggttctgct gatcgataat ggtaaaaaac cagggcgcaa aatccttatg tctggcggtg
    15001 ggcgctgcaa ctttaccaac ctttatgtcg aaccaggcgc ttatctgagc cagaatccgc
    15061 atttttgtaa gtctgcactc gcgcgtttta cccagtggga tttcattgat ctggtcaata
    15121 aacacggcat cgcctggcac gagaaaacgt taggacaact cttctgcgat gactccgcgc
    15181 agcagattgt cgacatgctg gtggatgagt gcgagaaggg caatgtaacc ttcagattgc
    15241 gtagcgaagt gctgagtgtg gcgaaggatg aaacaggctt cacgcttgaa ctgaacggca
    15301 tgactgtcgg ttgcgaaaag ctggtcatcg cgaccggtgg gctgtcaatg ccggggctgg
    15361 gcgcgtcgcc gtttggttat aagattgccg aacaatttgg cctcaacgtg ctgccgaccc
    15421 gcgcgggtct ggtgccattc actctgcata aaccgttgct cgaagagtta caggtgctgg
    15481 cgggcgtggc ggtgccttcc gtgattaccg ctgaaaacgg catcgttttc cgtgagaact
    15541 tactcttcac ccatcgcggc ttgtctggac cggcggtgtt gcagatttcc agctactggc
    15601 aaccgggtga atttgtcagt atcaatctgc taccggatgt ggatctcgaa accttcctga
    15661 atgagcagcg taacgcacat ccgaatcaga gcctgaaaaa cacactggcg gttcatctac
    15721 cgaagcggtt ggttgaacgc ttacagcaac tcgggcaaat cccggatgtt tcgctaaaac
    15781 agctcaacgt gcgtgaccaa caggcactga ttagcacatt gaccgactgg cgcgtacaac
    15841 ccaacggcac tgaaggctat cgcactgccg aagtgacgct cggcggcgtg gacaccaacg
    15901 aactctcttc acggacgatg gaagcgcgca aagtgcctgg gctgtacttc atcggcgaag
    15961 tgatggacgt taccggctgg ctggggggct ataacttcca gtgggcgtgg tcgagtgcgt
    16021 gggcttgtgc gcaggatttg attgcagcga agtcgtcctg acctgttacc taaagatgaa
    16081 aattattaga gccgcattaa aaatgagcta attttgatag tggctatctt gtgattattt
    16141 tctaatgagc ccgtgaactg aaaccctcca ggcttaatat aaggtggaag gaaaggtgat
    16201 tgaaaactca cccagtggcc agatctttat aaaatatgaa caggttgaaa aaaacagtaa
    16261 ctttgttgtt tttttatcct taaccactat gcattaatgc tgcgttattt catgatgcct
    16321 aagaaaaacc agcgttacgc aaatggtcaa cgctggtttt atccggtacg ttgcaattat
    16381 tttttagcag aacctgcttc taatattgaa acgattgaga acaacgtaaa gcataccgct
    16441 cctaaaccga ccaaaacccg cgaaggaata aaatatccca tgtcggtctg cgccatttcc
    16501 gcaagaaacg atgccaggaa aaggcaaaac aggcaggtga agacgggaat catcggtatt
    16561 ctgttggcta acgaacaggt acgtctccat accagtgcca gtagccagac ttttgagaat
    16621 atgctgtaac agatcattcc gaggcaaata agaataatgc cgggagccag tcgggcgctt
    16681 gcatcggaac tgactaagac gtatatcccc tgcagtaccg tgattgagcc gagaaaaata
    16741 acccaataac accacagcca gtgttcttta gttgaaaacg tattacgtgt ttgatggaca
    16801 attgtggcaa caaggccaat tagacaggca cagattgcgg ttaacccaag caatacgtga
    16861 cccgcgacat aatgcggagt tatgtcagca ctacgtaaca gcgtaataga ccaaatgaaa
    16921 ccgagcaggg tgagtaaaac gggaacggca atgaggcaat taccgattaa tgaagaataa
    16981 gcctgtaccg gcgttccatc gctcttgctc cccgctgcat ttttgggaat gagcagaaag
    17041 tgaccggatg acgctgccac cgtcgataca caggcggcaa tcataccaac gccgaaaata
    17101 acatggccgg cgacaaactc gtctgccatc acatcattgc ctgctaacag tgcccaaccc
    17161 catatcatgg tgataattga ccccgcatag ccaataatgg ggaacaatgt attgtaaaac
    17221 gtattaacgc cgcgcgtgag ctgcgaaatg ataataaatg cggtagtgaa taatgccagg
    17281 catatggctg ccagagaaat cagcacatga cccgcaacga aatactcact ttgcccagaa
    17341 ctttccagca caaagccacc taacgcgata caaattaagc ccataattaa agggattaat
    17401 ttgaaaagcc acccgatata tatgttcact ttaaactcct caggtatccc tgctaatatt
    17461 gaaatgtgca ttaatgataa tttcagcctg ttaatataat gacgagatat cattaaagac
    17521 aaatgcatag cataactata gcgggattta ctttgcagag cgctactttt cagcgttcaa
    17581 aaggccatgg taaataaata tttatcctaa gattaattct tattctgttg ttttttacaa
    17641 ttatcttcca gggag
//
LOCUS       DAZGLJ010000092        17533 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000092 DAZGLJ010000000
VERSION     DAZGLJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.092"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(86..1369)
                     /gene="gltA"
                     /locus_tag="ACHK49_002633"
     CDS             complement(86..1369)
                     /gene="gltA"
                     /locus_tag="ACHK49_002633"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO9263259.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1575..1682)
                     /locus_tag="ACHK49_002634"
     CDS             complement(1575..1682)
                     /locus_tag="ACHK49_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263260.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2063..2467
                     /gene="sdhC"
                     /locus_tag="ACHK49_002635"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2063..2467
                     /gene="sdhC"
                     /locus_tag="ACHK49_002635"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO9263261.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFI
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2461..2808
                     /gene="sdhD"
                     /locus_tag="ACHK49_002636"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2461..2808
                     /gene="sdhD"
                     /locus_tag="ACHK49_002636"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO9263262.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2808..4574
                     /gene="sdhA"
                     /locus_tag="ACHK49_002637"
     CDS             2808..4574
                     /gene="sdhA"
                     /locus_tag="ACHK49_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO9263263.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEVSLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4590..5306
                     /gene="sdhB"
                     /locus_tag="ACHK49_002638"
     CDS             4590..5306
                     /gene="sdhB"
                     /locus_tag="ACHK49_002638"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO9263264.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYDKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5607..8408
                     /gene="sucA"
                     /locus_tag="ACHK49_002639"
     CDS             5607..8408
                     /gene="sucA"
                     /locus_tag="ACHK49_002639"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO9263265.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8423..9640
                     /gene="odhB"
                     /locus_tag="ACHK49_002640"
     CDS             8423..9640
                     /gene="odhB"
                     /locus_tag="ACHK49_002640"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO9263266.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9734..10900
                     /gene="sucC"
                     /locus_tag="ACHK49_002641"
     CDS             9734..10900
                     /gene="sucC"
                     /locus_tag="ACHK49_002641"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO9263267.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLAITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10900..11769
                     /gene="sucD"
                     /locus_tag="ACHK49_002642"
     CDS             10900..11769
                     /gene="sucD"
                     /locus_tag="ACHK49_002642"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO9263268.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11873..12595)
                     /gene="mngR"
                     /locus_tag="ACHK49_002643"
     CDS             complement(11873..12595)
                     /gene="mngR"
                     /locus_tag="ACHK49_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFO9263269.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12764..14680
                     /gene="mngA"
                     /locus_tag="ACHK49_002644"
     CDS             12764..14680
                     /gene="mngA"
                     /locus_tag="ACHK49_002644"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFO9263270.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLGSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLMWRRH
                     AVKHGNYLTDGVMP"
     gene            14698..17331
                     /gene="mngB"
                     /locus_tag="ACHK49_002645"
     CDS             14698..17331
                     /gene="mngB"
                     /locus_tag="ACHK49_002645"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFO9263271.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YEMAVPRNLQERSARQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         4241 a         4375 c         4770 g         4147 t
ORIGIN      
        1 ttacaatttt ttgtgtttag gcatataaaa attaacccgc catttgaacg gcgggttaaa
       61 atatttacaa cttagcaatc aaccattaac gcttgatatc gcttttaaag tcgcgttttt
      121 catatcctgt atacagctga cgcggacggg caatcttcat accgtcactg tgcatttcgc
      181 tccagtgggc gatccagcca acggtacgtg ccattgcgaa aatgacggtg aacatggaag
      241 acggaatacc catcgctttc aggatgatac cagagtagaa atcgacgttc gggtacagtt
      301 tcttctcgat aaagtacggg tcgttcagcg cgatgttttc cagctccata gccacttcca
      361 gcaggtcatc tttggtgccc agctctttca gaacttcatg gcaggtttca cgcattacgg
      421 tggcgcgcgg gtcgtaattt ttgtacacgc ggtgaccgaa gcccatcagg cggaaagaat
      481 catttttatc tttcgcacga cgaacaaatt ccggaatgtg tttaacggag ctaatttctt
      541 ccagcatttt cagcgccgct tcgttagcac caccgtgcgc aggtccccac agtgaagcaa
      601 tacctgctgc gatacaggca aacgggttcg cacccgaaga gccagcggta cgcacggtgg
      661 aggtagaggc gttctgttca tggtcagcgt gcaggatcag aatacggtcc atagcacgtt
      721 ccagaatcgg attaacttca tacggttcgc acggcgtgga gaacatcata ttcaggaagt
      781 taccggcata ggagagatcg ttgcgcggat aaacaaatgg ctgaccaatg gaatacttgt
      841 aacacatcgc ggccatggtc ggcattttcg acagcaggcg gaacgcggca atttcacgat
      901 gacgaggatt gttaacatcc agcgagtcgt gatagaacgc cgccagcgcg ccggtaatac
      961 cacacatgac tgccattgga tgtgagtcgc gacggaaagc gtggaacaga cgggtaatct
     1021 gctcgtggat catggtatga cgggtcaccg tagttttaaa ttcgtcatac tgttcctgag
     1081 tcggtttttc accattcagc aggatgtaac aaacttccag gtagttagaa tcggtcgcca
     1141 gctgatcgat cgggaaaccg cggtgcagca aaataccttc atcaccatca ataaaagtaa
     1201 ttttagattc gcaggatgcg gttgaagtga agcctgggtc aaaggtgaac acaccttttg
     1261 aaccgagagt acggatatca ataacatctt gacccagcgt gcctttcagc acatccagtt
     1321 caacagctgt gtccccgttg agggtgagtt ttgcttttgt atcagccatt taaggtctcc
     1381 ttagcgcctt attacgtaag actgccggaa cttaaatttg ccttcgcaca tcaacctggc
     1441 tttacccgtt tgttatttgg ctcgccgctc tgtgaaagag gggaaaacct gggtacagag
     1501 ctctgggcgc ttgcaggtaa aggatccatt gatgacgaat aaatggcgaa tcaagtactt
     1561 agcaatccga attattaaac ttgtctacca ctaataactg tcccgaatga attggtcaat
     1621 acttccacac tgttacataa gttaatctta ggtgaaatac cgacttcata acttttacgc
     1681 attatatgct tttcctggta atgtttgtaa caactttgtt gaatgattgt caaattagat
     1741 gattaaaaat taaataaatg ttattatcgt gacctggatc actgttcagg ataaaacccg
     1801 acaaactata tgtaggttaa ttgtaatgat tttgtgaaca gcctatactg ccgccaggtc
     1861 tccggaacac cctgcaatcc cgagccaccc agcgttgtaa cgtgtcgttt tcgcatctgg
     1921 aagcagtgtt ttgcatgacg cgcagttata gaaaggacgc tgtctgaccc gcaagcagac
     1981 cggaggaagg aaatcccgac gtctccaggt aacagaaagt taacctctgt gcccgtagtc
     2041 cccagggaat aataagaaca gcatgtgggc gttattcatg ataagaaatg tgaaaaaaca
     2101 aagacctgtt aatctggacc tacagaccat ccggttcccc atcacggcga tagcgtccat
     2161 tctccatcgc gtttccggtg tgatcacctt tattgcagtg ggtatcctgc tgtggcttct
     2221 gggtaccagc ctctcttccc ctgaaggttt cgagcaagct tccgcgatta tgggcagctt
     2281 cttcgtcaaa tttatcatgt ggggcatcct taccgctctg gcgtatcacg tcgtcgtagg
     2341 tattcgccac atgatgatgg attttggcta tctggaagaa acattcgaag cgggtaaacg
     2401 ctccgccaaa atctcctttg ttattactgt cgtgctttca cttctcgcag gagtcctcgt
     2461 atggtaagca acgcctccgc attaggacgc aatggcgtac atgatttcat cctcgttcgc
     2521 gctaccgcta tcgtcctgac gctctacatc atttatatgg tcggtttttt cgccaccagt
     2581 ggcgaactga catatgaagt ctggatcggt ttcttcgcct ctgcgttcac caaagtgttc
     2641 accctgctgg cgctgttttc tatcttgatt catgcctgga tcggcatgtg gcaggtgttg
     2701 accgactacg ttaaaccgct ggcattgcgc ctgatgctgc aactggtgat tgtcgttgca
     2761 ctggtggttt acgtgattta tggattcgtt gtggtgtggg gtgtgtgatg aaattgccag
     2821 tcagagaatt tgatgcagtt gtgattggtg ccggtggcgc aggtatgcgc gcggcgctgc
     2881 aaatttccca gagcggccag acctgtgcgc tgctctctaa agtcttcccg acccgttccc
     2941 ataccgtttc tgcgcaaggc ggcattaccg ttgcgctggg taatacccat gaagataact
     3001 gggaatggca tatgtacgac accgtaaaag ggtcggacta tatcggtgac caggacgcga
     3061 ttgaatatat gtgtaaaacc gggccggaag cgattctgga actcgaacac atgggcctgc
     3121 cgttctcgcg tcttgatgat ggtcgtatct atcaacgtcc gtttggcggt cagtcgaaaa
     3181 acttcggcgg cgagcaggcg gcacgcactg cggcggcggc tgaccgtacc ggtcacgcac
     3241 tgttgcacac gctttatcag cagaacctga aaaaccacac caccattttc tccgagtggt
     3301 atgcgctgga tctggtgaaa aaccaggatg gcgcggtggt gggttgtacc gcactgtgca
     3361 tcgaaaccgg tgaagtggtt tatttcaaag cccgcgctac cgtgctggcg actggcgggg
     3421 cagggcgtat ttatcagtcc accactaacg cccacattaa cactggcgac ggtgtcggca
     3481 tggctatccg cgcaggcgta ccggtgcagg atatggaaat gtggcaattc cacccgaccg
     3541 gcattgccgg tgcgggcgta ctggtcaccg aaggttgccg tggtgaaggc ggttatctgc
     3601 tgaacaaaca tggcgaacgc tttatggagc gttatgcgcc gaacgccaaa gacctggcgg
     3661 gccgtgatgt ggtggcgcgt tccatcatga ttgaaatccg tgaaggtcgc ggctgtgatg
     3721 gtccgtgggg gccacacgcg aaactgaaac tcgatcacct gggtaaagaa gttctcgaat
     3781 cccgtctgcc gggtatcctg gagctttccc gtaccttcgc tcacgtcgat ccggtgaaag
     3841 agccgattcc ggttatccca acctgtcact acatgatggg cggtattccg accaaagtta
     3901 ccggtcaggc gctgactgtg aatgagaaag gcgaagatgt ggttgttccg ggactgtttg
     3961 ccgttggtga aatcgcttgt gtatcggtac acggcgctaa ccgtctgggc ggcaactcgc
     4021 tgctggacct ggtggtcttt ggtcgcgcgg caggtctgca tctgcaagag tctatcgccg
     4081 agcagggcgc actgcgcgat gccagcgagt ctgatgttga agtgtctctg gatcgcctga
     4141 accgctggaa caataatcgt aacggtgaag atccggtggc gattcgtaaa gcgctgcaag
     4201 aatgtatgca gcataacttc tcggtcttcc gtgaaggtga tgcgatggcg aaagggcttg
     4261 agcagttgaa agtgatccgc gagcgtctga aaaatgcccg tctggatgac acttccagcg
     4321 agttcaacac ccagcgcgtt gagtgcctgg aactggataa cctgatggaa acggcgtatg
     4381 caacggctgt ttctgccaac ttccgtaccg aaagccgtgg cgcacatagc cgcttcgact
     4441 tcccggatcg tgatgatgaa aactggctgt gccactccct gtatctgcca gagtcggaat
     4501 ccatgacgcg ccgaagcgtc aacatggaac cgaaactgcg cccggcattc ccgccgaaga
     4561 ttcgtactta ctaatgcgga gacaggaaaa tgagactcga gttttcaatt tatcgctata
     4621 acccggatgt tgatgatgct ccgcgtatgc aggattacac cctggaagcg gaagaaggtc
     4681 gcgacatgat gctgctggat gcgcttatcc agctgaaaga gaaagatccc agcctgtcgt
     4741 tccgccgctc ctgccgtgaa ggtgtgtgcg gttccgacgg tctgaacatg aacggcaaga
     4801 atggtctggc ctgtattacc ccgatttcgg cactcaacca gccgggcaag aagattgtga
     4861 ttcgcccgct gccaggttta ccggtgatcc gcgatttggt ggtagacatg ggacaattct
     4921 atgcgcaata tgacaaaatt aagccttacc tgttgaataa tggacaaaat ccgccagctc
     4981 gcgagcattt acagatgcca gagcagcgcg aaaaactcga cgggctgtat gaatgtattc
     5041 tctgcgcatg ttgttcaacc tcttgtccgt ctttctggtg gaatcccgat aagtttatcg
     5101 gcccggcagg cttgttagcg gcatatcgtt tcctgatcga tagccgtgat accgagactg
     5161 acagccgcct cgacggtttg agcgatgcat tcagcgtatt ccgctgtcac agcatcatga
     5221 actgcgtcag tgtatgtccg aaggggctga acccgacgcg cgccatcggc catattaagt
     5281 cgatgttgtt gcaacgtaat gcgtaaaccg taggcctgat aagacgcgca agcgtcgcat
     5341 caggcaacca gtgccggatg cggcgtgaac gccttatccg gcctacaagt cattacccgt
     5401 aggcctgata agcgcagcgc atcaggcgta acaaagaaat gcaggaaatc tttaaaaact
     5461 gcccctgaca ctaagacagt ttttaaaggt tccttcgcga gccactacat agacaagagc
     5521 tcgcaagtga accccggcac gcacatcact gtgcgtggta gtatccacgg cgaagtaagc
     5581 ataaaaaaga tgcttaaggg atcacgatgc agaacagcgc tttgaaagcc tggttggact
     5641 cttcttacct ctctggcgca aaccagagct ggatagaaca gctctatgaa gacttcttaa
     5701 ccgatcctga ctcggttgac gctaactggc gttcgacgtt ccagcagtta cctggtacgg
     5761 gagtcaaacc ggatcaattc cactctcaaa cgcgtgaata tttccgccgc ctggcgaaag
     5821 acgcttcacg ttactcttca acgatctccg accctgacac caatgtgaag caggttaaag
     5881 tcctgcagct cattaacgca taccgcttcc gtggtcacca gcatgcgaat ctcgatccgc
     5941 tgggactgtg gcagcaagat aaagtggccg atctggatcc gtctttccac gatctgaccg
     6001 aagcagactt ccaggagacc ttcaacgtcg gttcatttgc cagcggcaaa gaaaccatga
     6061 aactcggcga gctgctggaa gccctcaagc aaacctactg tggcccgatt ggtgccgagt
     6121 atatgcacat taccagcacc gaagaaaaac gctggatcca acagcgtatc gagtctggtc
     6181 gcgcgacttt caatagcgaa gagaaaaaac gcttcttaag cgaactgacc gccgctgaag
     6241 gccttgaacg ttacctcggc gcaaaattcc ctggcgcaaa acgcttctcg ctggaaggcg
     6301 gtgacgcgtt aatcccgatg cttaaagaga tgatccgcca cgctggcaac agcggcaccc
     6361 gcgaagtggt tcttgggatg gcgcaccgtg gtcgtctgaa cgtgctggtg aacgtgctgg
     6421 gtaaaaaacc gcaagacttg ttcgacgagt ttgccggtaa acataaagaa cacctcggca
     6481 cgggtgacgt gaaataccac atgggcttct cgtctgactt ccagaccgat ggcggcctgg
     6541 tgcacctggc gctggcgttt aacccgtctc accttgagat tgtaagcccg gtagttatcg
     6601 gttctgttcg tgcccgtctg gacagacttg atgagccgag cagcaacaaa gtgctgccaa
     6661 tcaccatcca cggtgacgcc gcagtgaccg ggcagggcgt ggttcaggaa actctgaaca
     6721 tgtcgaaagc gcgtggttat gaagttggcg gtacggtacg tatcgttatc aacaaccagg
     6781 ttggtttcac cacctctaat ccgctggatg ctcgttctac accgtactgt actgatatcg
     6841 gtaagatggt tcaggcaccg attttccacg ttaacgcgga tgatccggaa gccgttgcct
     6901 ttgtgacccg tctggcgctc gatttccgta acacctttaa acgtgatgtc ttcatcgacc
     6961 tggtgtgcta ccgccgtcac ggccacaacg aagccgacga gccgagcgca acccagccgc
     7021 tgatgtatca gaaaatcaaa aaacatccga caccgcgcaa aatctacgct gacaagctgg
     7081 agcaggaaaa agtggcgacg ctggaagatg ccaccgagat ggttaacctg taccgcgatg
     7141 cgctggatgc tggcgattgc gtagtggcag agtggcgtcc gatgaacatg cactctttca
     7201 cctggtcgcc gtacctcaac cacgaatggg acgaagagta cccgaacaaa gttgagatga
     7261 agcgcctgca ggagctggcg aaacgcatca gcacggtgcc ggaagcagtt gaaatgcagt
     7321 ctcgcgttgc caagatttat ggcgatcgcc aggcgatggc tgccggtgag aaactgttcg
     7381 actggggcgg tgcggaaaac ctcgcttacg ccacgctggt tgacgaaggc attccggttc
     7441 gcctgtcggg tgaagactcc ggtcgcggta ccttcttcca ccgccacgcg gtgatccaca
     7501 accagtctaa cggttccact tacacgccgt tgcaacacat ccataacggg cagggcgcgt
     7561 tccgcgtctg ggactccgta ctgtctgaag aagcagtgct ggcgtttgaa tacggttatg
     7621 ccaccgcaga accacgcact ctgaccatct gggaagcgca gttcggtgac ttcgccaacg
     7681 gtgcgcaggt ggttatcgac cagttcatct cttctggcga acagaaatgg ggccggatgt
     7741 gtggtctggt gatgttgctg ccgcacggtt acgaagggca ggggccggag cactcctccg
     7801 cgcgtctgga acgttatctg caactttgtg ctgagcaaaa catgcaggtg tgcgtaccgt
     7861 ctaccccggc acaggtttac cacatgctgc gtcgtcaggc gctgcgcggg atgcgtcgtc
     7921 cgctggtcgt gatgtcgccg aaatccctgc tgcgtcatcc gctggcggtt tccagcctcg
     7981 aagaactggc gaacggcacc ttcctgccag ccatcggtga aatcgacgag cttgatccga
     8041 agggcgtgaa gcgcgtagtg atgtgttctg gtaaggttta ttacgacctg ctggaacagc
     8101 gtcgtaagaa caatcaacac gatgtcgcca ttgtgcgtat cgagcaactc tacccgttcc
     8161 cgcataaagc gatgcaggaa gtgttgcagc agtttgctca cgtcaaggat tttgtctggt
     8221 gccaggaaga gccgctcaac cagggcgcat ggtactgcag ccagcatcat ttccgtgaag
     8281 tgattccgtt tggggcttct ctgcgttatg caggccgccc ggcctccgcc tctccggcgg
     8341 tagggtatat gtccgttcac cagaaacagc aacaagatct ggttaatgac gcgctgaacg
     8401 tcgaataaat aaaggataca caatgagtag cgtagatatt ctggtccctg acctgcctga
     8461 atccgtagcc gatgccaccg tcgcaacctg gcataaaaaa cccggcgacg cagtcgtacg
     8521 tgatgaagtg ctggtagaaa tcgaaactga caaagtggta ctggaagtac cggcatcagc
     8581 agacggcatt ctggatgcgg ttctggaaga tgaaggtaca acggtaacgt ctcgtcagat
     8641 ccttggtcgc ctgcgtgaag gcaacagcgc cggtaaagaa accagcgcca aatctgaaga
     8701 gaaagcgtcc actccggcgc aacgccagca ggcatctctg gaagagcaaa acaacgatgc
     8761 gttaagcccg gcgatccgtc gcctgctggc tgaacacaat ctcgacgcca gcgccattaa
     8821 aggcaccggt gtgggtggtc gtctgactcg tgaagatgtg gaaaaacatc tggcgaaagc
     8881 cccggcgaaa gagtctgctc cggcagcggc tgctccggcg gcgcaaccgg ctctggctgc
     8941 acgtagtgaa aaacgtgtcc cgatgactcg cctgcgtaag cgtgtggcag agcgtctgct
     9001 ggaagcgaaa aactccaccg ccatgctgac cacgttcaac gaagtcaaca tgaagccgat
     9061 tatggatctg cgtaagcagt acggtgaagc gtttgaaaaa cgccacggca tccgtctggg
     9121 ctttatgtcc ttctacgtga aagcggtggt tgaagccctg aaacgttacc cggaagtgaa
     9181 cgcgtctatc gacggcgatg acgtggttta ccacaactat ttcgacgtca gcatggcggt
     9241 ttctacgccg cgcggcctgg tgacaccggt actgcgtgat gtcgataccc tcggcatggc
     9301 agacatcgag aagaaaatca aagagctggc agtcaaaggc cgtgacggca agctgaccgt
     9361 tgaagatctg accggtggta acttcaccat caccaacggt ggtgtgttcg gttccctgat
     9421 gtctacgccg atcatcaacc cgccgcagag cgcaattctg ggtatgcacg ctatcaaaga
     9481 tcgtccgatg gcggtgaatg gtcaggttga gatcctgccg atgatgtacc tggcgctgtc
     9541 ctacgatcac cgtctgatcg atggtcgcga atccgtgggc ttcctggtaa cgatcaaaga
     9601 gttgctggaa gatccgacgc gtctgctgct ggacgtgtag tagtttaagc ttcacctgca
     9661 ctgtagaccg acatggatag ggtgtcggtc tacggtttaa aagataacga ttactgaagg
     9721 atggacagaa cacatgaact tacatgaata tcaggcaaaa caactttttg cccgctatgg
     9781 cttaccagca ccggtgggtt atgcctgtac tactccgcgc gaagcagaag aagccgcttc
     9841 aaaaatcggt gccggcccgt gggtagtgaa atgtcaggtt cacgctggtg gccgcggtaa
     9901 agcgggcggt gtgaaagttg taaacagcaa agaagacatc cgtgcttttg cagaaaactg
     9961 gctcggcaag cgtctggtaa cgtatcaaac agatgccaat ggccaaccgg ttaaccagat
    10021 tctggttgaa gcagcgaccg atatcgctaa agagctgtat ctcggtgccg ttgtagaccg
    10081 tagttcccgt cgtgtggtct ttatggcctc caccgaaggc ggcgtggaaa tcgaaaaagt
    10141 ggcggaagaa actccgcacc tgatccacaa agttgcgctt gatccgctga ctggcccgat
    10201 gccgtatcag ggacgcgagc tggcgttcaa actgggtctg gaaggtaaac tggttcagca
    10261 gttcaccaaa atcttcatgg gcctggcgac cattttcctg gagcgcgacc tggcgttgat
    10321 cgaaatcaac ccgctggcca tcaccaaaca gggcgatctg atttgcctcg acggcaaact
    10381 gggcgctgac ggcaacgcac tgttccgcca gcctgatctg cgcgaaatgc gtgaccagtc
    10441 gcaggaagat ccgcgtgaag cacaggctgc acagtgggaa ctgaactacg ttgcgctgga
    10501 cggtaacatc ggttgtatgg ttaacggcgc aggtctggcg atgggtacga tggacatcgt
    10561 taaactgcac ggcggcgaac cggctaactt ccttgacgtt ggcggcggcg caaccaaaga
    10621 acgtgtaacc gaagcgttca aaatcatcct ctctgacgac aaagtgaaag ccgttctggt
    10681 taacatcttc ggcggtatcg ttcgttgcga cctgatcgct gacggtatca tcggcgcggt
    10741 agcagaagtg ggtgttaacg taccggtcgt ggtacgtctg gaaggtaaca acgccgaact
    10801 cggcgcgaag aaactggctg acagcggcct gaatattatt gcagcaaaag gtctgacgga
    10861 tgcagctcag caggttgttg ccgcagtgga ggggaaataa tgtccatttt aattgataaa
    10921 aacaccaagg ttatctgcca gggctttact ggtagccagg ggactttcca ctcagaacag
    10981 gccattgcat acggcactaa aatggttggc ggcgtaaccc caggtaaagg cggtactacc
    11041 cacctcggcc tgccggtgtt caacaccgtg cgtgaagctg ttgctgctac tggcgctacc
    11101 gcttctgtta tctacgtacc agcaccgttc tgcaaagact ccattctgga agctattgat
    11161 gcaggcatca aactgattat caccatcact gaaggcatcc cgacgctgga tatgctgact
    11221 gtgaaagtga aactggacga agccggcgtt cgtatgatcg gcccgaactg cccgggcgtt
    11281 atcactccgg gtgaatgcaa aatcggtatc cagccgggtc acattcacaa accgggtaaa
    11341 gtgggtatcg tttcccgttc cggtacactg acctatgaag cggttaaaca gaccacggat
    11401 tacggtttcg gtcagtcgac ctgcgttggt atcggcggtg acccgatccc tggctctaac
    11461 tttatcgaca ttctcgaaat gttcgaaaaa gatccgcaga ccgaagcgat cgtaatgatc
    11521 ggtgagatcg gcggtagcgc tgaagaagaa gcggcagcgt acatcaaaga gcatgttacc
    11581 aagccagttg tgggttacat cgctggtgtg actgcgccga aaggcaaacg tatgggccac
    11641 gcgggtgcca tcattgccgg tgggaaaggg actgcggatg agaaattcgc tgctctggaa
    11701 gccgcaggcg tgaaaaccgt tcgcagcctg gcggatatcg gtgaagcact gaaaactgtt
    11761 ctgaaataaa tatctgtaat aagaaatagc cctcgccgct tccctctaca ggaatggcga
    11821 agggctgtcg gtttcgacat ggttggccat cgtatgatgg ccttttttgt gcttatcgcg
    11881 atgattttcg ttgcgctatc agggtaaatt tatagtcatc ggtattaaaa gcgttgcggc
    11941 tatattcaaa cacccgacca tcaactaaat atccacgcga tactttttca agaatcggct
    12001 ttgtctggct gatattaagc agacggctca tctcttcggt tggcatcaga ggaatgattt
    12061 cctgttcgct acgatcgata accattttct tcacttcttc aataaagtga tattttgaat
    12121 tttccatgac ctgccaggtg agatccggga acaacgcaag cggcatccag gtttcttcca
    12181 gcgccattgg cttttgcttg cgatagcgca cgcgcttcac atgccacaca cgatcctgcg
    12241 cggtgatttg tagctgttgc tgaagaaaat cgtcagccgg aatcacttca aatatcagga
    12301 cttcactgtg tgtatcgacg tgacggtccg acagtttttc atcaaaactg gttaactgaa
    12361 aaatatcgta attgacccgc tcttctttga cgtaagtccc gctgccctga atgctttcga
    12421 ggatctgttg ctcgactaac tggcgcaaag cctgacgcac cgtaacccgg ctgacgccaa
    12481 actctgtttg taatgccgat tcggtaggta acgcatcgcc aggtttaagc tcgccacgcg
    12541 caatttgttc acgaatgcga tcggcaatct gccggtataa gggcttgtgt cccattttta
    12601 gtatctcatt aatacgaatt tagccattat gcccgataaa ttcatcctgt aaataataca
    12661 aatacaatac aaataatttc aatcaagtga aattgatcac ataatggtat tgttttatcg
    12721 ggcacactgg cgcgactata aaaacgatca agtgaggatc atgatgaatc tgacgactct
    12781 gacccaccgc gatgcgttgt gtctgaatgc gcgctttacc agccgtgaag aggccatcca
    12841 cgcgttgact caacgtcttg ctgctctggg gaaaatttcc agtactgagc aatttctgga
    12901 agaagtgtat cgccgtgaaa gccttggccc gaccgcctta ggtgaagggt tggctgtgcc
    12961 gcatggcaaa actgctgcgg taaaagaagc ggcgtttgcg gtcgccacac tcagcgagcc
    13021 gcttcagtgg gaaggcgttg atggcccgga agcggttgat ttagtggtgc tgctggcgat
    13081 tccccccaat gaagcgggta ctacgcatat gcaactgctg acagcgctga ccacgcgcct
    13141 ggcggatgat gagattcggg cgcgtataca gtcggcgacg acgcctgatg agttgctctc
    13201 ggcgctggat gacaagggag gcacgcaacc ttctgcctct ttttccaacg cgccaactat
    13261 cgtctgcgta acggcctgtc cggcgggtat tgctcacacc tatatggctg cggaatatct
    13321 ggaaaaagcc ggacgcaaac tcggcgtaaa tgtttacgtt gaaaaacaag gcgctaacgg
    13381 cattgaaggg cgtttaacgg cggatcaact caatagtgca accgcctgta tttttgcggc
    13441 tgaagtcgcc atcaaggaga gtgagcgttt taatggcatt cccgcgcttt cagtgcctgt
    13501 tgccgagccg attcgccatg cagaagcgtt gatccaacaa gcgcttaccc tcaagcgtag
    13561 cgatgagacg cgtaccgtac agcaagatac gcaaccggtg aaaagtgtca aaacggagct
    13621 gaaacaggca ctgttgagcg gaatctcttt tgccgtaccg ttgattgtcg cggggggcac
    13681 ggtgctggcg gtcgcggtat tactgtcgca aatcttcggg ctacaagatc tgtttaatga
    13741 agaaaactcc tggctgtgga tgtaccgcaa gctgggcggc gggctgctcg gaattttgat
    13801 ggtaccggtg ctcgcggcct ataccgccta ttctctggca gataaaccgg cgttagcgcc
    13861 aggctttgcg gctggacttg ccgccaacat gatcggctcc gggtttctcg gcgcggtcgt
    13921 tggcggattg atagccggtt acttgatgcg ctgggtgaaa aatcacttgc gtcttggcag
    13981 taaattcaat ggcttcctga ctttttatct ctacccggtg ctcggtacgt tgggagcggg
    14041 cagtctgatg ctgtttgtgg tgggagaacc tgtcgcatgg atcaataact cgcttaccgc
    14101 ctggctgaac ggtctgtcag gaagtaacgc gctgttgctg ggtgccattc tcggttttat
    14161 gtgttccttt gaccttggag ggccagtgaa taaagccgct tatgcattct gcctgggcgc
    14221 aatggcgaac ggcgtttacg gcccgtatgc cattttcgcc tccgtcaaaa tggtttcggc
    14281 atttaccgta accgcttcca cgatgctcgc accgcgcctg tttaaagagt ttgaaattga
    14341 gaccgggaaa tccacctggc tgttagggct ggcaggtatt accgaagggg cgatcccgat
    14401 ggcgattgaa gatccgctgc gggttattgg ttcgtttgtg ctgggctcta tggtaacggg
    14461 cgctattgtc ggtgcgatga atatcggcct ttcgacaccc ggtgccggca ttttctcgct
    14521 ctttttactt catgataatg gcgcgggcgg tgttatggcg gcaattggct ggtttggtgc
    14581 ggcattggtg ggggctgcaa tctcgactgc aattctcctg atgtggcggc gtcacgcggt
    14641 taagcatggc aactatctga ctgatggcgt aatgccataa acaaaaagga aacgacgatg
    14701 aaagcggtat ctcgcgttca catcaccccg catatgcact gggatcggga gtggtatttc
    14761 accaccgaag agtcacgcat tctgctggtc aataatatgg aagagatcct gtgccgactg
    14821 gaacaggaca acgaatacaa atattacgta ctcgacgggc aaacggcgat cctcgaagat
    14881 tatttcgcgg tgaaaccgga aaacaaagac cgtgtgaaga aacaggtaga agccggcaag
    14941 ttgattatcg gcccctggta tacccagacc gataccacga ttgtttctgc ggaatccatc
    15001 gtccgtaatc tgatgtacgg aatgcgtgac tgcctcgcgt ttggcgagcc gatgaaaata
    15061 ggttatttac cggattcctt tggcatgtcc gggcagcttc cgcatatcta caatggattt
    15121 ggcattaccc gcaccatgtt ctggcgtgga tgttctgagc gccacggtac tgataaaacc
    15181 gagtttttgt ggcaaagcag tgacggtagc gaagtgacgg cgcaagtgtt gccgctgggc
    15241 tacgccatcg gtaagtactt acctgccgac gaagacggat tacgcaagcg cctcgacagt
    15301 tattttgatg tgctggaaaa agcgtcagta accaaagaaa ttttattgcc gaatggtcat
    15361 gaccagatgc cattgcagca aaatatcttc gaagtgatgg ataagctacg tgagatctac
    15421 cctcaacgta agtttgtgat gagccgcttt gaagaggtat ttgagaagat cgaagcgcag
    15481 cgagataatc tggcaaccct gaaaggggaa tttattgatg gcaaatatat gcgcgtgcat
    15541 cgcaccatcg gttctacgcg tatggatatc aaaattgccc acgcgcgtat tgaaaataag
    15601 attgttaatc tgctggaacc gctggcaaca ctggcctgga cgttgggttt tgaataccac
    15661 cacggcttgc tggagaaaat gtggaaagag atcttaaaaa atcatgccca cgacagtatc
    15721 ggttgttgtt gcagtgacaa agttcatcgc gaaatcgtcg cccgcttcga actggctgaa
    15781 gacatggcgg ataatctgat tcgtttctac atgcgcaaaa ttgccgacaa catgccgcag
    15841 agcgacgccg acaaactcgt cctgtttaac ctgatgccct ggccgcgtga agaagtgatc
    15901 aacaccactg tgcgcctgcg cgccagccag tttaatttgc gggacgatcg cggccagcct
    15961 gtgccgtatt ttattcgcca tgcccgtgag atcgatccag gcctaatcga tcggcaaatt
    16021 gttcattatg gcaattatga tccctttatg gagttcgaca tccagatcaa ccagattgtc
    16081 ccttctatgg gctatcgcac gctttatatt gaagcgaatc agcctggcaa cgtgattgcg
    16141 gcaaaaagtg acgctgaagg gattctggaa aatgctttct ggcagattgc gctcaatgag
    16201 gatggttctc tgcaactggt agataaagac agcggtgtgc gctatgaccg ggtattgcaa
    16261 attgaagaaa gctctgatga tggtgatgaa tatgactatt cacccgcaaa agaagagtgg
    16321 gtaattaccg cggcgaacgc gaaaccgcaa tgcgatatta ttcatgaagc ctggcagagc
    16381 agggctgtta tccgttatga aatggcggtg ccgcgtaatc tgcaagaacg cagcgcaaga
    16441 caatccactg gcagagtagg ggtggagatg gttgtcactc tgagtcataa cagcagacgt
    16501 attgatgtgg atatcaacct tgataaccag gctgacgatc atcgccttcg cgtcctgatc
    16561 cctacacctt ttaacaccga cagtgttctg gcagatacgc agtttggttc gctaacgcgc
    16621 cccgtgaacg acagtgcaat gaacaactgg cagcaagaag gctggaaaga agcgccggtt
    16681 ccggtatgga atatgctcaa ctatgttgcc ttacaggaag ggcgtaacgg catggctgtc
    16741 tttagcgaag ggctacgtga atttgaagtc atcggtgaag agaagaaaac ctttgccatt
    16801 acgttgctgc gtggcgtggg cttactgggc aaagaagatc tgcttttaag gcctgggcgg
    16861 ccttcgggaa ttaaaatgcc agtcccggac tcacaactac gtggtctgct ttcttgtcgc
    16921 ctaagtttat tgagttatac cggtacgcca accgccgctg gtgtagctca gcaggcgcga
    16981 gcatggctga ctccagtaca gtgttacaac aaaatcccat gggatgcgat gaagctcaac
    17041 aaagccggat tcaacgtgcc ggaaagttat agtttgttga aaatgccccc agtgggatgc
    17101 ctgataagcg cacttaagaa agctgaagac cgacaagaag tgattttacg gctgtttaat
    17161 ccggctgaat cagcaacctg tgatgcgact gttgctttca gtcgcgaggt gatttcttgc
    17221 tcagaaacga tgatggatga acacattacc accgaggaaa atcaaggttc aaatctatcg
    17281 gggccttttt tacccggcca gtcacggacg ttcagttacc ggcttgcctg aatagcaatc
    17341 aaaccgaagc cacatatgcg cggccagatt gttgacaaag ggcgctttgt tcatgccgga
    17401 tacggcatga acgctttatt cggtctacaa aagcaggcaa attcaatata ttgcagagat
    17461 gatgtagaca ctggcaagcg tagcgcatca ggtaattttg cgtttatctt cactctcaag
    17521 ccacgtatat gtg
//
LOCUS       DAZGLJ010000093        17457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000093 DAZGLJ010000000
VERSION     DAZGLJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.093"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(162..1752)
                     /gene="lsrK"
                     /locus_tag="ACHK49_002646"
                     /pseudo
     CDS             complement(162..1752)
                     /gene="lsrK"
                     /locus_tag="ACHK49_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
     gene            complement(1831..2784)
                     /gene="lsrR"
                     /locus_tag="ACHK49_002647"
     CDS             complement(1831..2784)
                     /gene="lsrR"
                     /locus_tag="ACHK49_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO9263272.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3033..4568
                     /gene="lsrA"
                     /locus_tag="ACHK49_002648"
     CDS             3033..4568
                     /gene="lsrA"
                     /locus_tag="ACHK49_002648"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO9263273.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLEALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASPKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSMTLNAGEILGLAGLVGAGRTELAETFYGLRTLRSGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIS
                     AQNVAVLLISSDLEEIELMTDRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4562..5590
                     /gene="lsrC"
                     /locus_tag="ACHK49_002649"
     CDS             4562..5590
                     /gene="lsrC"
                     /locus_tag="ACHK49_002649"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO9263274.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5590..6582
                     /gene="lsrD"
                     /locus_tag="ACHK49_002650"
     CDS             5590..6582
                     /gene="lsrD"
                     /locus_tag="ACHK49_002650"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO9263275.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6594..7616
                     /gene="lsrB"
                     /locus_tag="ACHK49_002651"
     CDS             6594..7616
                     /gene="lsrB"
                     /locus_tag="ACHK49_002651"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO9263276.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7643..8479
                     /gene="lsrF"
                     /locus_tag="ACHK49_002652"
                     /pseudo
     CDS             7643..8479
                     /gene="lsrF"
                     /locus_tag="ACHK49_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462959.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
     gene            8478..8972
                     /locus_tag="ACHK49_002653"
                     /pseudo
     CDS             8478..8972
                     /locus_tag="ACHK49_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(8976..9890)
                     /gene="yneE"
                     /locus_tag="ACHK49_002654"
     CDS             complement(8976..9890)
                     /gene="yneE"
                     /locus_tag="ACHK49_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO9263277.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDIQFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10097..11548)
                     /gene="uxaB"
                     /locus_tag="ACHK49_002655"
     CDS             complement(10097..11548)
                     /gene="uxaB"
                     /locus_tag="ACHK49_002655"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO9263278.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLARQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(11775..13193)
                     /locus_tag="ACHK49_002656"
     CDS             complement(11775..13193)
                     /locus_tag="ACHK49_002656"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO9263279.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(13332..13691)
                     /gene="yneG"
                     /locus_tag="ACHK49_002657"
     CDS             complement(13332..13691)
                     /gene="yneG"
                     /locus_tag="ACHK49_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO9263280.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(13691..14617)
                     /gene="glsB"
                     /locus_tag="ACHK49_002658"
     CDS             complement(13691..14617)
                     /gene="glsB"
                     /locus_tag="ACHK49_002658"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO9263281.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(14681..16069)
                     /gene="sad"
                     /locus_tag="ACHK49_002659"
     CDS             complement(14681..16069)
                     /gene="sad"
                     /locus_tag="ACHK49_002659"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO9263282.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16170..17051
                     /gene="yneJ"
                     /locus_tag="ACHK49_002660"
     CDS             16170..17051
                     /gene="yneJ"
                     /locus_tag="ACHK49_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9263283.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17129..>17457
                     /gene="yneK"
                     /locus_tag="ACHK49_002661"
     CDS             17129..>17457
                     /gene="yneK"
                     /locus_tag="ACHK49_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO9263284.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVIN"
BASE COUNT         4398 a         4468 c         4327 g         4264 t
ORIGIN      
        1 ctttaagaaa aatagcaatt ttcccttgaa tatagtactg gtgatggaac gatgaatctg
       61 caagctggca tttaacaagc cagctctaaa agaagggaaa taagaaaaac tatactcaaa
      121 aactaacagc cacggtcatc atgatgtggc cgtcaatgaa actataaccc aggcgctttc
      181 cataacgacg tcgtcagtcc atgatcaacc agccccagct gatcctgata aactgcctgc
      241 cacttatcgc gtgaatcctg ataaagttca tgcttttccg ggtctggtgt gtgcgtccgt
      301 tcccagcgaa ccaggcgttc tccggtttct gccattgatg aaaaaattcc ggcaccgacg
      361 ccagctgcaa tggcacatcc taatgcggtg gcttctttga ctaccggaat attgacgggt
      421 aatcccgaga catcagcgag aatttgactc cataatttcc cttttgaacc tccgccagca
      481 aaaaccagcg aggtgggatg aatattcgag aaatcagcaa tttgctgcaa gttacacgct
      541 gatacaatcg ccgcattttc ttccagcgca cggaacaatg tcgctttgtt acatttatcc
      601 gggtcaatgg acaagttaat aaaggaaggc gcagcgtgat accaggtttt aaagcgcatt
      661 ctgtcggaga agatcggcat tacgccccac gacccaggcg gcacccgact ggccatctct
      721 tccagcagcg tataggtgtc gatgcctaaa cgttcggcaa tcagtttttc ttcggcacag
      781 aaagcatcgc ggaaccagcg catggtgagt ccggtaaaaa agcttataga ttcagcttgt
      841 accatgccag gaataacatg aggattaacg cgcacgttca tttctgggtc tgtcaccggc
      901 gcggctaaat ttacaacttg ctgccagaat gtgccgccaa gaaccgcggt ttgtgccgga
      961 cgcacaacgc ctaacccaag gcaaccaagc tgcacgtcgc ctcctccaac gaccaccgga
     1021 gtgcccgcct tcagaccgca gagttccgcc gcttgtgaac ttaccacgcc cagcaatgtg
     1081 ccggtttctt tgacaggaga aagaatatcg gcacgcaggc cagccatatc cagcaatgca
     1141 ggtttccagt cacgggtggt tagatcaaag tcccgtggtg ccagcgttag agggatccac
     1201 cgccagttcg ccgctgagca tataggccag ccagtcgctg atcatggtga tggttgatgc
     1261 ctgacggtaa atatcggaac gatggtgcgc cagccaaagt aatctgggga tggcacttaa
     1321 agccagtgtt tgtccggtcg cgcgataaac ttcgttttca aaggtattgt tgtgcagttc
     1381 tttaagttcg ctaacttcgc gcgctgctct ggcatccaca ttggcgcagg cccagatcgg
     1441 tgttccttca ttgttatata aaacaatgcc ttcacgcata gaacatgccg aaacggcagc
     1501 gatatactcc gggtctatgc cggcgttgtg cagcgcctgg cgcatacact cacacgccag
     1561 ttgccagttt ttgttgagat caaattccat agaaccagga acgtccggta ctgccagatg
     1621 ccgccactcc gcctgtccca ctgctatttg attgcctttc aggtcgaaaa tcacagcccg
     1681 aatacttccg gtgcctgcat ccagcgccaa caggtaatac tttgattctg aaggggtaaa
     1741 gagtcgagcc attagattat cctcggctta tgcgttctgt ctgttcctct atacgttctc
     1801 catcattccc ggtaataagg tcatgcaaat ttaactacgt aaaatcgccg ctgctgtatc
     1861 ctgatcggta accagtgcgt tgatataacc gcctttcatt gcagcggcaa ttgcttcggc
     1921 tttattttct ccccctgcca cgccaacccg gacgggtatg gtcttcagcg cgcttaaagg
     1981 taagccaatc agttcgttat gtattttaat atccgtgaca acgtcacctt ttgcatcaaa
     2041 aaagtagcct aaaatgtcgc caaccgcccc ttttcgacca atcattaact gttcgccctg
     2101 gctgatataa ccggagcgaa tgatcgtcgc gtcgtcctgt tgactgacgg ccccaatgcc
     2161 gacaatcgcc acatccgctg cttgcgcggc taacagaaca tctttgacgc aattttcatt
     2221 ttttagcgta cgggcaatgt cagcggagga tgcccgcaac ggagccggaa taatattcac
     2281 actgcacgcc gcgttaagct gcccgatacc cgtcatataa gaaccgacgc caccggagag
     2341 cgtgaccagg cgaatttgct gtgacgaaat aaaaccactt aagcgttgca gcgtattcat
     2401 ggttgcctcg ccaaaaccaa tcgccagcat ctgttgtggt tgaagtaaac tcatcaacat
     2461 atgcgccgcg cctatcccta gtcgcccgcc gacatcagca tccgcaagcc cagggatcac
     2521 ccggacatgt tgcagcgaaa actgacgacg taattgagtt tcatattcca gacagccttc
     2581 aaagcgagaa ttaatctgta cgcgaataat gccggactga tgccctttct ccagcaagcg
     2641 cgacactttc aaacgggtca ggccgagacg atcgctgatc tcgctctggg tcagcccgtc
     2701 gtgatagtaa aaccacgcga tccgcgcgac ctgttcttct tcacacattc cctgttctga
     2761 aattgccgaa tcgttgattg tcataattca ttcttcactt tgaacatatt taaatcttta
     2821 atgcaattgt tcagttcttg cgcatttata tctgtgatgg cagccacagt ttgactctac
     2881 gagcatgaac aaacgcaacc gtgaaaatca aaatagcata aattgtgatc tattcgtcgg
     2941 aaatatgtgc aatgtccacc taaggttatg aacaaattaa aagcagaaat acatttgttc
     3001 aaaactcacc tgcaaaactg aacgggggaa atatgcaaac gagtgatacc cgcgcgttac
     3061 cgctactttg cgcccgctcg gtttataaac agtattcagg ggtcaatgtc ctgaaaggca
     3121 tcgattttac gttgcatcag ggggaggtcc acgccctgct cggcggcaat ggtgccggta
     3181 aatcgacgtt aatgaagatt attgccggta ttacccctgc tgatagcggt acgctggaga
     3241 ttgggggcaa caactacgcc agattaacgc cagttcatgc tcatcagctg ggcatttatc
     3301 tcgttcccca ggaaccgctg cttttcccaa gcctgtcgat aaaagaaaac atcctgtttg
     3361 ggatggcaaa aaaacagctc tccatgcaaa aaatgaagaa cttgctggag gcgctgggct
     3421 gccagtttga tctgcatagt ctggcaggat cgctggatgt cgccgatcgc caaatggtgg
     3481 aaatcctccg cgggctaatg cgcgactcgc ggattctgat cctcgatgaa cctaccgcct
     3541 cgcttacccc tgcggaaacc gaacgcttgt ttactcgctt gcaagagctg cttgctactg
     3601 gcgtgggtat tgtttttatc tcgcataagc tgccggaaat tcgccagatt gccgatcgaa
     3661 ttagcgtgat gcgcgacgga accatcgcct taagcggcaa aaccagcgaa ctgtctaccg
     3721 acgacattat tcaggccatc acgccagtgg tacgggaaaa atcgctctct gccagcccaa
     3781 aattatggct ggagttacct ggtaaccgcc cacaacatgc tgccggaacg ccggtgctga
     3841 cactggaaaa tctgaccggc gaaggtttca ggaatgtcag catgacgctc aatgccggag
     3901 aaattctggg cctggctggg ctggtggggg ccggacgcac agaactggcc gagacgttct
     3961 atggtctgcg tactttgcgt agcggacgca ttatgctgaa tggtaaagag atcaataaat
     4021 tatccactgg agaacgttta ctgcgcggtc tggtttatct gccggaagat cgccagtcat
     4081 ccggactgaa tctcgatgct tcgctggcct ggaacgtctg cgcccttact cataaccttc
     4141 gtggattctg ggcgaaaacc gcgaaagata atgccaccct ggaacgttat cgtcgggcgc
     4201 tgaatattaa atttaaccaa ccggaacaag ctgcacggac attatccggt ggcaaccagc
     4261 aaaaaatcct cattgccaaa tgcctggaag cttcgccgca agcattgatt gtcgatgagc
     4321 cgacgcgcgg cgtggatgtc tcggcccgta atgatatcta ccagctgttg cgcagcatct
     4381 ccgcacagaa tgtggctgtg ctgcttatct cctctgatct ggaagagatc gaactgatga
     4441 cagatcgtgt gtatgtgatg catcagggcg aaattaccca ctctgcactg accgggcgcg
     4501 atattaatgt cgagactatt atgcgcgttg ccttcggcga tagtcagcgt caggaggcat
     4561 catgctgaag tttattcaga acaaccgtga aatcacggca ttgctggcgg tggtgctgct
     4621 gtttgtatta cccggttttc tcgaccgtca gtatttaagt gtgcaaacgc tgaccatggt
     4681 ttatagcagc gcgcaaatcc tgatcctgct ggcaatgggc gcgacactgg taatgcttac
     4741 gcgcaatatt gatgtttcag tgggttcgat taccggaatg tgcgcggtgc tgttggggat
     4801 gttactgaac gcaggatatt cactacctgt tgcctgtgtc gcgactttac tgcttggttt
     4861 gctcgcggga tttttcaacg gtgtcctggt cgcgtggcta aagatccctg ccattgttgc
     4921 cacccttggc acgttagggt tgtacagagg catcatgttg ctgtggactg gcggtaaatg
     4981 gattgaaggg ttacccgccg aactgaaaca gctctccgca ccgctgctgt ttggcgtttc
     5041 cgcgattggt tggttgacga taattctggt ggcatttatg gcctggctgc tggcaaagac
     5101 ggcgtttgga cgcagttttt atgccacggg cgataattta cagggcgctc gtcaactggg
     5161 tgttcgtacg gaagccattc gcattgtggc attttcgttg aacggctgca tggcggcact
     5221 ggcgggaatt gtgtttgctt cgcagattgg ttttatcccc aaccagaccg gtaccgggct
     5281 ggagatgaaa gcaattgcag cctgcgtgct gggcggcatt agtttgctcg gcggttccgg
     5341 tgcgatcatc ggtgcggtac tcggcgcatg gttcctgacg cagatcgata gcgtactggt
     5401 gctgttgcgc attccggcat ggtggaacga ttttatcgcg ggcctcgtgc tgctggcggt
     5461 gctggtgttt gatggacgcc tgcgttgtgc gctggaacgt aatctacggc ggcaaaaata
     5521 tgcccgcttt atgacgccac cgccatccgt taaacccgct tcgtcaggta aaaaacggga
     5581 ggccgcataa tgcgtattcg ctacggttgg gaactggctc ttgccgcact gctcgttatt
     5641 gagattgtcg catttggtgc aattaacccg cgaatgttag atctcaatat gttgctgttc
     5701 agcaccagtg actttatctg cattggcatt gtcgccctac cgctgacgat ggtgattgtc
     5761 agtggcggga tcgatatttc gtttggttcg accatcggcc tctgcgccat tgcattgggc
     5821 gtactgtttc aaagtggtgt gccgatgccg ctggcgatac tcctgacctt actgctcggc
     5881 gcattgtgcg ggctgatcaa cgccggatta attatctata ccaaagttaa cccgctggtg
     5941 attacgcttg gcacgctgta tctgtttgcc ggaagcgctc tgctgctttc cggtatggcc
     6001 ggagcgacgg ggtacgaagg tattggtgga ttcccgatgg cgtttacaga tttcgctaac
     6061 ctggatgtgc tgggactccc cgttccgctg attatcttcc tgatatgtct cctcgttttc
     6121 tggctctggc tgcataaaac ccatgccgga cgtaatgtgt ttttgattgg gcaaagcccg
     6181 cgcgtggcgc tttatagcgc gattccagtt aaccgcacct tatgtgcgct ctatgccatg
     6241 acggggctgg cgtctgcggt cgccgctgtg ctgctggtat cgtattttgg ttcagcacgt
     6301 tccgatctcg gtgcgtcgtt tctgatgccc gccatcaccg ccgtggtgct tggcggggcc
     6361 aatatttatg gtggttccgg ttccattatc ggcaccgcca ttgcggtttt attagtggga
     6421 tatttgcagc aaggtttgca aatggcagga gtgccaaatc aggtgtccag cgccctttcc
     6481 ggtgcgctac ttatcgtcgt tgtcgtaggt cgttccgtta gcctgcatcg ccagcaaatt
     6541 aaagagtggc tggcgcgtcg ggccaataac ccattgccat aaaggatatc ttcatgacac
     6601 ttcatcgctt taagaaaatc gccttactta gcgctcttgg cattgccgca atctctatga
     6661 atgtgcaggc cgcagagcgt attgcattta ttcccaaact ggttggcgtg ggatttttta
     6721 ccagcggtgg caacggcgca caacaagcgg gtaaagagct gggcgttgat gtgacctacg
     6781 acgggccgac agaacccagt gtttctggtc aggtacagtt gattaataac ttcgtcaatc
     6841 aaggttataa cgccattatc gtttctgcgg tttcgcctga tggcttgtgt ccggcactga
     6901 aacgcgccat gcaacgtggt gtgagagtgc tgacctggga ctctgatact aaaccggagt
     6961 gccgctctta ctacattaat cagggaacgc ccgcccagtt gggaggtatg ttggtggata
     7021 tggcggcgcg tcaggtgaat aaagacaaag ccaaagtcgc gtttttctac tcaagcccca
     7081 ccgttacgga ccaaaaccag tgggtgaaag aagcgaaagc gaaaatcgcc aaagagcatc
     7141 ctggctggga aattgtcact acgcagtttg gctataacga tgccactaaa tcattacaaa
     7201 ccgcagaagg aatattaaaa gcgtatagcg atctcgacgc cattatcgcc cccgacgcca
     7261 acgccctgcc cgctgccgca caagccgcag aaaacctgaa aaatgacaaa gttgcgattg
     7321 tcggattcag tacgccaaac gtgatgcgtc catatgtgga acgcggcacg gtgaaagaat
     7381 ttggcctgtg ggatgtggtt cagcaaggca aaatttcagt gtatgtcgcg gatgcattat
     7441 tgaaaaaagg atcaatgaaa acgggcgaca agctggatat ccagggcgta ggtcaggtgg
     7501 aagtctcgcc aaacagcgtt cagggctatg actacgaagc ggatggtaat ggcatcgtac
     7561 tgttaccgga gcgcgtgata ttcaacaaag agaatatcgg caaatacgat ttctgatgtg
     7621 cattacttaa ccggagtaag ttatggcaga tttagacgat attaaagatg gtaaagattt
     7681 tcgtaccgat caatcgcaac aaaatatccc ttttatcctg aaaggttgcg gtgcgctgga
     7741 ttggggaatg cagtcacgct tatcgcggat atttaatccg aaaacgggta aaaccgtgat
     7801 gctggccttt gaccatggtt attttcaggg gccgactacc ggacttgaac gcattgatat
     7861 aaatatcgcc ccgctgtttg aacatgccga tgtattaatg tgtacgcgcg gcattttgcg
     7921 cagcgtagtt cccccagcga ccaataagcc ggtggtactg cgggcgtcag gagcgaactc
     7981 tattctggcg gaattaagta atgaagccgt ggcgttatcg atggatgatg ccgtgcgcct
     8041 gaacagttgc gcggtggcgg cgcaggttta tatcggcagc gaatatgaac atcagtcgat
     8101 caaaaatatt attcagctgg ttgatgccgg aatgaaagtg ggaatgccga ccatggccgt
     8161 gactggcgtg ggcaaagata tggtgcgcga tcagcgttat ttctcgctcg cgactcgaat
     8221 cgccgctgaa atgggggcgc aaattatcaa aacctattat gtcgaaaaag gttttgaacg
     8281 gattgttgcc ggatgtccgg tacccattgt tattgctggc ggtaaaaaat taccggagcg
     8341 cgaggcgctg gaaatgtgct ggcaggctat cgatcagggc gcttctggtg tggatatggg
     8401 gcgtaatatt ttccagtctg accatccggt ggcgatgatg aaagccgtac aggcggtggt
     8461 tcaccataac gaaacggcaa ccggaacagg cactcgatct gatttttgct aatgcctcac
     8521 tgcaatggct gcccgaccac tacgaattgt ttcctcatct ggtttcttta cttaatccgc
     8581 agggcgtgct ggcagtacag atgccagata actggctgga gccgacccat gtgctcatgc
     8641 gcgaagttgc ctgggaacaa aactacccgg atcgcgggcg tgagccgttg gctggcgttc
     8701 atgcttacta cgatattttg agcgaagccg gatgtgaggt cgatatctgg cgaaccactt
     8761 actatcacca gatgccgtcg caccaggcga ttatcgattg ggtgaccgcc actggattac
     8821 gtccgtggtt acaggatctg accgagagcg aacagcagct ttttcttacg cgctaccatc
     8881 agatgctgga agagcagtat ccactgcaag agaacggaca gatactgctg gcatttccgc
     8941 gtctgtttat tgttgcccgc cgtacggagt aattatcacg tcagctggta atgacgatcg
     9001 ggaagaattt ttgctggaat ttctgcttca tcgttcatct gtaacaggtc aatttcaata
     9061 gcgttgcaga tggcatccag cggtaaatca ttgttttcag taccgaacgg atcttccagt
     9121 tcttccgcca gacaatccag cgaaataaaa gtgtaggaaa tcagcacaga gataaaaggc
     9181 gtcatgtaat gcaggtccac gaccagcgcg aacggcagca tgatacaaaa cagataaacg
     9241 gtacgatgca aaatcagggt gtaggcaaag ggaattggcg tgtaggcaat gcgctcacag
     9301 cctgccagaa ccgctgaaat atcattaagc cgatcgttga ggctaataaa ctggatatct
     9361 gaaagctgcc cattgcggcg ctgaaccgcc aaccactccc ccattattaa caagatacgg
     9421 ttagctggag agttcgaagc cagtacaggc tgaagatctt cagtcttgag ataatgagcc
     9481 agcacttccg cctgtggctg tttgcgtaat gtcatgcgta aacagtgggc gaaggcgatt
     9541 tgcaaccggg caaactccct tacacttgcc gaatccggca atgtcgtttt tacctcgcgc
     9601 agtaacgacc gtgaggcaat catcaactgc ccccaaagtt ttcgcgcttc aacgtaacgg
     9661 gcgtacccgg cattattacg aaaaccaaga aaaatggcga tggcgacacc gagaatgctg
     9721 aacggtgcga gggtgaattt gatgcccaga tgcgtgtacc agggcagcat gaaaataaca
     9781 gcgatagaaa agagaaaatt gagtagtaag cgcgatgata tcttggataa tactgagccg
     9841 tgccagacaa aaatacggcg cagccagtgt tgttgtggac gaacaatcat ggttatcttc
     9901 aggcgtgaaa aagtcgccct attaaacgtg attacgatca cattctcaag acgctactta
     9961 caaattacct acaagcttac aacattaaca ggcgctgcat gtggcatctg ttaatgataa
    10021 aggcacatag gatgttgtaa ctaatatagt gaaataagaa acccggtcga aaccgggttc
    10081 agaagtaacg gtgttattag cacaacggac gtacagcttc gcgcatccct ttttcaagaa
    10141 tcgcatccag gtcattagca acctgctcga ccagaccagg cacttgcgtc aggtcctgct
    10201 cccagtggtc tttttctgcc aacacaatcg ctaccagttc ctgggtaccg ataacacggt
    10261 cacgatgctg gctccagagt tgctgataac gttccagcca gtgtgcatcg tcctgtaccg
    10321 gatatgtttc tccgttacgc tcaccacgat agaaggcaat caatgccgct aatgcgaaag
    10381 taaggcgcgc cggaagtgtg ccttttgcct tctgccgtgc cagcagctgc ggcaggatgc
    10441 gagtgcggaa cttggtcata ccgttgagcg cgatagacag cagctgatgc ttaatgtacg
    10501 ggttacggaa acgcccggtg actgcactgg cgaaagattc cagttcatca cgaggcaaat
    10561 ccagtaccgg aataatttct tcgtagatag ctttttcaac gaatgcgcaa atttcagcat
    10621 cgttcatcgc ttcacctacg gtatccagcc ctgcctggaa cgccaccggc accagcgcgg
    10681 tgtgcgcacc gttgaggatt gccactttgc gttctttgta cggtttaata tcgtcaacaa
    10741 tcagcacgtt gagcggatat ttgtccagac gcagttcagt cgctaaggat ttcggtccct
    10801 gaatcacaaa caggtaaaag tgttcagcgg tgtcgagaaa accatcgtga taacccagct
    10861 cttcttccag tttagccact tcatcgcgcg gataaccggt aacgatacgg tccaccagcg
    10921 tagaacagaa gctgttagcc tgatccagcc attgaataaa tgcttctggc agtgcccact
    10981 cttgcgcata gcgcagcacc agttcacgca acgcgtcgcc attgtagtcg atcaactcac
    11041 acgggatgat gatccaacct ttatccagcg caccgttgaa atggctaaaa cgttcgaaca
    11101 gcagacgggt cagttttgcc ggataactta ccgctggcgc gtcatcgaat ttatcgcccg
    11161 catggtagct gatacccgct tcggtggtat tagagaaaac aaagcgcatt tccgggttgt
    11221 gagccagttt caggaattca tcgtattcac tgtagacgct gatttcacga ttaaccgagc
    11281 gaatcagacg cgcgtcgctg accgcttccc ccttctcatt caggccacgg ataatggtgg
    11341 tatacagacc atcctgcgtg ctcagtgacg gtgggaatga agtttcaatc ggacgaacaa
    11401 cgaccacacc agaattcaga tcggtgtgct cattcaggag atcgatttgc cagtcaacaa
    11461 aggcgcgcag gaagttacct tcaccaaact gaatgatacg ttctggatac tgtgcaccgg
    11521 gaaaatcgcg acgatttagt gttttcacaa tgggttccct tctgattagt catacaacct
    11581 gtttgaattg gtacgacagg ttagcaaact ttaatacgcc gaacccctgt tttgatcaac
    11641 tcctgatgat taatgagcag ttttatgaga aaagtgtggc gcggatcatg gtttaatcga
    11701 ggaaatacgc cttttcctgg atcataaagt ggtagaacac attgcattca aatcgcgcgg
    11761 aatgaataaa gatgtcagac aacttcctca ccgtaacgca tagtgctggt acggttgcgc
    11821 ccatctttct tcgagcgata cagacacgta tcggcttcga ccatcaattt attaaaatca
    11881 tcggtcagcg ttcggtacga tgcgcgacca ctaccgacgc caatacttac cgtgagataa
    11941 agcgtttttt gttgccaggt gaatggttgc agttcaacgc ctttacggat tttttccgcc
    12001 attagcagac catcgacagg attcaccgac ggcactgcaa cagcaaattc ttcgccgccc
    12061 atgcgcgcca ccagcccctt atcaccgaca atcttctgaa tatgccgggc aaacacgctt
    12121 aacactttat cgccacattc atgtccatag ttatcgttaa tgcttttgaa atagtcgata
    12181 tcaagcagca tgaccgtcag atgttgtgtc tgtttcagcg atggactttt caacgcctca
    12241 taaagaccgg accgtgagta aacctgagtc agaaaatcaa agtcggctcg cagcgcaact
    12301 tgcttcatta gcgaattgat cgctgccacg ctaaaagaaa ccataattgg gcatatcgcc
    12361 atcgtggcaa taccgagacg tgcggagaac atttccggaa tggagaacgg cgaaccgacc
    12421 gagatatcaa tcaccgaatt tgccaccagt acgatttcca ccgcaccggt gacaaaggtc
    12481 aacagacatg ttacctgcgg cgtatagcgc actgcacacc agattaatgc aggcagcggg
    12541 aacgccagac tccccgctcc gccaatgact accgaggcaa taaccgacac aataagcgcg
    12601 atggcgggca tcatctgttc tgctttaaag cgcggcagca ctccaggaat agccaacgtc
    12661 agcatacaag gcacgatcaa cacgcccgtt gagaattgct cactgaacca gtccgcaagc
    12721 aaaggccaga aatccagact atcaatactg accgaaccaa tcgccccgac aatagcgcat
    12781 aataatgcgg caatcagaca gtaattaaat agccgtaagg cacttactgg ctcatactta
    12841 tttttcccca gacgcttatc ccgcacgacc agtaaggcga cggtaacaat aaacatcata
    12901 ttggagaaat tgataaccag tgaaaccagt ccccattcgg tggttatggc atcataaacc
    12961 agcatcgcca cataactgat cgcatagtaa tgcagtcgat taagccagac atagcgggca
    13021 aacacccccg ccattacgcc attcagcggc cagaaaagag aaagttcgtg aaccagccgt
    13081 aactctgcac caataaaata gaacagcgtg gttagcgcaa aaatagcgat gctattacgt
    13141 agcagatgac cttcctggaa caaacggaaa gttgaaatgg ggtgtacatg cattaattat
    13201 ctgtccatac aattgcgggt ggcaattatc tcaacgtaat aagtaaattc tcataaagga
    13261 tagcatgttc gctaacagcg gcggcaaata aaggtaccct gggaatgcta ttttatcgtt
    13321 ttccgcaatt ctcatggctg attcatctgt acaaccagcc agtgatagat taccgccacg
    13381 atataacgtt gttgttcttc gcttaacgaa acaccctgcg gaatgttatg ccacttagcc
    13441 agacaaccgc gacagcaggt cgcggtggcg tgctgagcga taaacaccgg atgcccgcgc
    13501 atgggggttt gcttaccgtc attagccggt aatgccggag caagacgttt agcgacaaaa
    13561 tccgccgcat gttgttcgat gactggcgcg cctttctcca ggcaatactg acgctctttc
    13621 atgcccagac gaaagcgaga gcgaaatttt gaacgggata aacgcgcgaa gagtggatcg
    13681 agagactgca ttaataaacc gaacgcccta actgtttcgt caattgttca aggacggcaa
    13741 tacccgcaag cgagttacct gcatcatcca gttccggact ccagacagcg atggccattt
    13801 catgcggaac aatcgccaca ataccgccac caacgccaga tttcgccggt agccccaccc
    13861 gccaggcaaa ctcccccgcg ttctggtaca taccactggt cgccatcagc gcgttaattt
    13921 gccgcgcctg cattggcgtc accacaggtt catcaatatg aatagctttc ccctgattag
    13981 ccagaaagac aaacgtcctg gccagctcta cacagctcat tttcagagcg cagtaatgaa
    14041 agtagttttg cagaacggtt gtcacatcat gatggaaatt gccaaacgac ttcatcagcc
    14101 aggcgatagc cgcatttcgc gcggaatgtt caaattcgga acgcgctacc accgtatcgt
    14161 atgaaatatc agacacaccg cttaagccgc gcacgacttc cagcatacgt tgccgtggtg
    14221 cgcttaatcg cccttgcaac atatcgcaaa ccaccagcgc accagcatta ataaacggat
    14281 tacgcggaat accctgctcc atttccagtt gcactaaaga attgaacggt gatccagacg
    14341 gatctttgcc gacgcgttgc cagatttcct cttcggagta atgacgcatg gcgacaacga
    14401 gactcagcac tttggaaata gactgaatgg aaaaacgttc ttgcgcgtct ccggcctgaa
    14461 aaagctgtcc gtcaacggta cagatagcaa tgcccaatcg ggaaccgtct actgtagcca
    14521 gcgccggaat ataatccgcg actttaccct gaccaatgag cggccgcact tgccgcaaga
    14581 tgttttctaa aattgcatta tccatggcga ctgccacttt ctactcctgg accgcaggtc
    14641 tgaaaagacc tgcgagtata tcagagctga atatgtcgcg tcagatccgg tctttccaca
    14701 ccgtctggat attacagaat tcgtgtaagc cgaaatggga aagctcacga ccaaagccac
    14761 tctttttcac gccaccaaag gccactcgcg cgtcgctggc acaataacca ttgataaaca
    14821 ccccaccgca ttccagacgt gccgccatct gtctggcctg tgtttcgtcg gtggtaaaaa
    14881 tggtcgctga aaggccgaac tcactatcat tagccagttc cagcgcatgt tctgcatctt
    14941 tcgcaacggt gattgccgca acagggccaa acatttcttc ccgaaacgcg gtcatttctg
    15001 gggtaacatt cgccagaacc gttggcggat agtagttacc tgccccagcc atcttttccc
    15061 cgcccagtaa caaacgcgca ccctgcgcca gggttttctc cacctgatga tgcagctcat
    15121 cacgtaaatc aaaacgagcc attggtccga gagcattctc ttcatcacgg ggatcgccca
    15181 ttttcaaggc tgccgcagct gccacaaaac gttcggtaaa tgcagaagca attccctctt
    15241 cgataataaa gcgttttgcc gctgcacaaa cctgtccggt attctgataa cgtccggcta
    15301 ccgccgcttt aaccgccagt tccagatcgg catcgttaag cacaataaat ggatccgaac
    15361 cgcccagttc cagtacgcat tttttcagtg ccgctccagc ctgtgcgcca atagccgctc
    15421 ccgcacgaac acttccggtc accgtgacag cagcaatgcg cgaatcttta atcatttgac
    15481 tgacaccgtc gttgtcggca ttcagccagc catatacgcc ttgcgggatt cccgcatctt
    15541 taaacacctg ggcaatgagc tgagcacagc ccatcacatt cggcgcatgt ttaagtaagt
    15601 agccgttacc tgcaagaatg atgggaaccg cgccacgcat cacctgccat aacggaaagt
    15661 tccacggcat aatcgccaga atcgtcccca acggtcgata ctcaataact gcctgctgat
    15721 tttccaccag cgtaggttcc gccttcagca ttgccggacc atgttctgca taccagtcac
    15781 acaaattcgc cgatttcgcc acttcagcgc gcgcctgatt gattggtttg cccatttcac
    15841 gggtgatcat ttgcgccatt tcttcgctac gggcgcgcag agccttaccg ataccacgca
    15901 gtttttcagc acgataatct atatttgtct cgcgccagtc gcgaaagcct gctgccgcca
    15961 gctgaagtgc gttttcgata tcgttagcgc cagcccacgg cagcacagaa agttgttcac
    16021 ccgtggcagg atttattgaa attgcatgag ttgccggagt aatggtcatc ggggtatctc
    16081 ctttatgagt catggtatga agatacgcag atttactctt gctttaaaat gaataatatt
    16141 aagccactta ttcacgaatc gagaatgtta tggatctgac ccaactggag atgttcaacg
    16201 cggttgccga agctggcagc attacccagg ctgcagcaaa agtgcatcgc gtaccgtcga
    16261 atctcactac ccgtctacgc cagctggaaa cagaactggg ggtagatctg tttattcgcg
    16321 agaatcagcg tttacgtctc tctcctgccg ggcataactt tttacgctat agccaacaaa
    16381 ttctcgcgtt agtggatgaa gcgcggagcg ttgtcgctgg cgatgagccg caaggtttat
    16441 tttctcttgg ttcgctggaa agcaccgcag cagtgcgcat tccggccacg ctggcggaat
    16501 tcaaccatcg ttatcccaaa attcagtttt cactttccac cggcccttcc ggcactatgc
    16561 tggaaggagt gctggaggga aaactgaatg cggcatttat tgatgggccc attaaccata
    16621 ctgccattga cgggataccg gtataccgcg aggagctgat gatcgtcacg ccacaaggtc
    16681 atgcgccagt aatccgcgcc agtcaggtta acggcagtaa catttatgcc ttccgcgcca
    16741 attgttcgta tcgtcgccac ttcgagagct ggtttcatgc tgacggtgcc gctccgggaa
    16801 ctattcacga gatggagtcg tatcacggga tgttggcctg tgtggtcgca ggagcaggca
    16861 ttgcgcttat tccacgctct atgctggaaa gtatgccggg gcatcaccag gttgaagcgt
    16921 ggccgttagc tgagcaatgg cgttggttaa caacctggct ggtctggcgt cgcggtgcga
    16981 aaacgcgtcc gcttgaggca tttattcaac tactggatgt gcctgactcg gcaaaacagg
    17041 gttaccaatg agctattttt gatagttctt gcagctcaaa tgccctatat agtgatgttc
    17101 cgatgactta tgactatatg gggcaaatat ggttacgcca gtaagcatca gtaattacat
    17161 atctcttcct gatgattttc ccgtacgtaa tattgcacct caggtaaaag aagttttaaa
    17221 ggactttatt gatgcgctaa gtacaataat atgtgatgaa gagtggcgca cgtctttaaa
    17281 catcaactca gccacaaaaa agatatttaa taaccttgat aacttatcat atattcagag
    17341 gacttctttt cggggtaacg acacgctata caatgaaaag gttcagttta aactcactta
    17401 tcccgtcaag aatggaagac acaaagaaaa tattgaattt caggtagtaa taaattt
//
LOCUS       DAZGLJ010000094        17446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000094 DAZGLJ010000000
VERSION     DAZGLJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.094"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            89..607
                     /gene="yfiR"
                     /locus_tag="ACHK49_002662"
     CDS             89..607
                     /gene="yfiR"
                     /locus_tag="ACHK49_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO9263285.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            597..1823
                     /gene="dgcN"
                     /locus_tag="ACHK49_002663"
     CDS             597..1823
                     /gene="dgcN"
                     /locus_tag="ACHK49_002663"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO9263286.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAKKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            1839..2321
                     /gene="yfiB"
                     /locus_tag="ACHK49_002664"
     CDS             1839..2321
                     /gene="yfiB"
                     /locus_tag="ACHK49_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO9263287.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2398..2745)
                     /gene="rplS"
                     /locus_tag="ACHK49_002665"
     CDS             complement(2398..2745)
                     /gene="rplS"
                     /locus_tag="ACHK49_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO9263288.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(2787..3554)
                     /gene="trmD"
                     /locus_tag="ACHK49_002666"
     CDS             complement(2787..3554)
                     /gene="trmD"
                     /locus_tag="ACHK49_002666"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO9263289.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(3585..4133)
                     /gene="rimM"
                     /locus_tag="ACHK49_002667"
     CDS             complement(3585..4133)
                     /gene="rimM"
                     /locus_tag="ACHK49_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO9263290.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4152..4400)
                     /gene="rpsP"
                     /locus_tag="ACHK49_002668"
     CDS             complement(4152..4400)
                     /gene="rpsP"
                     /locus_tag="ACHK49_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO9263291.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4649..6010)
                     /gene="ffh"
                     /locus_tag="ACHK49_002669"
     CDS             complement(4649..6010)
                     /gene="ffh"
                     /locus_tag="ACHK49_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO9263292.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTIKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6177..6968
                     /gene="ypjD"
                     /locus_tag="ACHK49_002670"
     CDS             6177..6968
                     /gene="ypjD"
                     /locus_tag="ACHK49_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO9263293.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6989..8275
                     /gene="yfjD"
                     /locus_tag="ACHK49_002671"
     CDS             6989..8275
                     /gene="yfjD"
                     /locus_tag="ACHK49_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO9263294.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8330..8923)
                     /gene="grpE"
                     /locus_tag="ACHK49_002672"
     CDS             complement(8330..8923)
                     /gene="grpE"
                     /locus_tag="ACHK49_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO9263295.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            9046..9924
                     /gene="nadK"
                     /locus_tag="ACHK49_002673"
     CDS             9046..9924
                     /gene="nadK"
                     /locus_tag="ACHK49_002673"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO9263296.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            10010..11671
                     /gene="recN"
                     /locus_tag="ACHK49_002674"
     CDS             10010..11671
                     /gene="recN"
                     /locus_tag="ACHK49_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO9263297.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            11820..12161
                     /gene="bamE"
                     /locus_tag="ACHK49_002675"
     CDS             11820..12161
                     /gene="bamE"
                     /locus_tag="ACHK49_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO9263298.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12223..12513)
                     /gene="yfjF"
                     /locus_tag="ACHK49_002676"
     CDS             complement(12223..12513)
                     /gene="yfjF"
                     /locus_tag="ACHK49_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO9263299.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12503..12940)
                     /gene="ratA"
                     /locus_tag="ACHK49_002677"
     CDS             complement(12503..12940)
                     /gene="ratA"
                     /locus_tag="ACHK49_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO9263300.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            13111..13593
                     /gene="smpB"
                     /locus_tag="ACHK49_002678"
     CDS             13111..13593
                     /gene="smpB"
                     /locus_tag="ACHK49_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO9263301.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            13808..14170
                     /gene="ssrA"
                     /locus_tag="ACHK49_002679"
     tmRNA           13808..14170
                     /gene="ssrA"
                     /locus_tag="ACHK49_002679"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14441..14689
                     /locus_tag="ACHK49_002680"
                     /pseudo
     CDS             14441..14689
                     /locus_tag="ACHK49_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
     gene            15347..16708
                     /gene="espK"
                     /locus_tag="ACHK49_002681"
     CDS             15347..16708
                     /gene="espK"
                     /locus_tag="ACHK49_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFO9263302.1"
                     /translation="MLPTSQLRPSGTFYSNSAETSAGIKSEITPTQIEEARAIGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDIPSTVKN
                     LHIELTDSPFIHIISEGIECLTVCHCHISGVPESVRHLEIRGYATDGIKNVPNGLLSL
                     SINRYTPENQARIDSLISPSLQTLSLTGCSNIILPEKLPESLRSVTIHVEQKTTWNIV
                     AEGVPDGLDLDLQNVLLSPNVIKEKNITFQGNALDAALHFRPGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIVIQNTLTNAVWDARTPRKYKQDSLIKRALNEHERGTKFKQCLKNH
                     NNYNVIMADLSIYNRDKLWAKTSKAGLEFQTLIRNRTVMFCADGLVNSLKLIANKSEG
                     YGQSITASELRWIYRNKDNSQIMKNIKFYLHGKEIPAERILDTPEWKDYRPKYSGSTY
                     KYS"
     gene            complement(17085..>17446)
                     /locus_tag="ACHK49_002682"
     CDS             complement(17085..>17446)
                     /locus_tag="ACHK49_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO9263303.1"
                     /translation="IILCRGTTGIQAENIVEYKTAGGLPDSNPNVKAPDEYMAQQQVR
                     IGRILPEYTSDLSVADRFSREHYLIVVKVKAKYITRGSVTESGWVIDKTAPVEPLAII
                     DRTFGMKENISMVNASK"
BASE COUNT         4636 a         4463 c         3964 g         4383 t
ORIGIN      
        1 gatgacgatc gcatgaaaaa atgatatttt aatgagcatt ctgaaaaaat atcgatgcat
       61 ttcgagcgaa gatggtgggg atccctgaat gcgtttttct caccgactgt tcttacttct
      121 gattctcctg ctgacgggag caccaatact cgctcaggag ccttctgacg ttgcgaaaaa
      181 cgtgcgtatg atggtttccg gtattgtcag ttacacccgc tggcctgcgc tatcagggcc
      241 accaaagtta tgtatctttt catcttcacg ttttagtacc gccctacaag agaatgccgc
      301 gacatctttg ccttatctgc ctgtcatcat tcacactcaa caagaggcga tgatttcagg
      361 ctgcaatggt ttttattttg gcaacgagtc accgacattc cagatggaat taaccgaaca
      421 atatccgtca aaggcgttat tattaattgc cgaacaaaat accgagtgca ttattggtag
      481 cgccttttgt ctgatcatcc acaataatga cgtcagattt gccgtaaacc tggatgcctt
      541 atcgcgtagc ggtgtaaagg tcaacccgga tgtcttaatg ctcgcacgga agaaaaatga
      601 tggataacga taattctctt aataagcgcc ccacgtttaa aagagcatta cgcaacatca
      661 gtatgaccag catatttatc actatgatgc tgatctggtt gctgctttcc gtgacctcgg
      721 tgctgaccct gaaacagtac gcgcaaaaaa acctggcact gacagcagca acaatgactt
      781 acagtctgga agcagctgtc gtttttgccg atggccctgc agcaactgaa acactggcag
      841 cgctgggcca gcaagggcaa ttttcaactg cagaagtacg tgataagcag caaaatattc
      901 tggcgtcctg gcattacacc cgtaaggatc caggcgatac tttcagcaat ttcataagcc
      961 actggctctt ccccgccccc atcattcagc cgattcgtca caatggtgaa accattggcg
     1021 aagtacgctt aaccgctcgc gacagttcaa tcagccattt tatctggttt tcgctcgccg
     1081 tactgaccgg ttgtattctg ctggcatcag gcatcgcaat taccctcacc cgccatttgc
     1141 acaatggcct ggtagaagcg ctgaaaaata tcaccgatgt cgtacatgat gtgcgttcca
     1201 accgcaattt ttcccgacga gtttcggaag aacgtatcgc tgagtttcac cgcttcgctc
     1261 tcgacttcaa cagtctgctg gatgaaatgg aagagtggca gcttcgttta caggccaaaa
     1321 atgcgcagct tctgcgtacc gcgctacatg acccattaac cgggctggct aaccgcgcag
     1381 cgtttcgtag cggcatcaat acgttgatga acaattccga tgcccgaaaa acgtcggcgt
     1441 tactatttct tgatggcgat aatttcaaat atatcaatga tacctggggt catgcgacgg
     1501 gcgatagagt cttgattgaa atcgcaaaac ggttagctga atttggcggg ctgcgacata
     1561 aagcataccg cctgggcggc gatgaattcg ctatggtgct ctatgatgta cagtcggaat
     1621 ctgaagtgca gcagatatgc tcagcactga cacaaatctt taatctcccg tttgatcttc
     1681 ataatggcca tcagaccacc atgacattaa gcattggtta cgcgatgacc attgagcacg
     1741 cctctgcgaa aaaattacaa gagcttgccg atcacaatat gtatcaggcc aaacaccagc
     1801 gtgccgaaaa gctggtgaga taacaaggat atatcgatat gataaagcac ctggtagcac
     1861 ccctgatttt cacctcacta atactgactg gctgccagtc ccctcaggga aagtttactc
     1921 ctgagcaagt cgccgctatg caatcttatg gatttactga atccgccggc gactggtcgc
     1981 tgggcttatc agatgccatt ctgttcgcaa aaaatgacta caaattgctc ccggaaagcc
     2041 agcaacagat ccaaaccatg gcagccaaat tggcctcgac agggctaaca catgcccgta
     2101 tggatggaca caccgataac tatggtgaag acagttacaa cgaaggctta tcattgaaac
     2161 gggcgaatgt cgtggccgat gcatgggcta tgggtggaca aattccgcgc agcaatctca
     2221 ccacacaggg tttaggaaaa aaatatccca tagccagtaa caagaccgcc cagggccgcg
     2281 ccgagaaccg ccgcgtcgca gtggtgatta ctacccctta aaacaccagc aaacagataa
     2341 aaaaagggcc agccaattgg ccagcccttc ttaacaggat gtcgcttaag cgaaatctta
     2401 gttaagacgc tctttgatac gagcagcctt accagtacgc tcacgcaggt agtacagttt
     2461 agctttacga acagcaccac gacgtttgac agaaatgctg tcaactaccg gagagtgagt
     2521 ctggaagaca cgctcaacgc cttcgccgtt ggaaatttta cgaacagtga atgcagagtg
     2581 cagaccgcgg ttacgaatag cgataaccac gccctcgaat gcctgcagac gttttttgga
     2641 accttcaaca acccatactt tcacttccac ggtatcaccc ggacggaagg aaggtacgtc
     2701 ctgcttcatc tgctcttgtt caagttgctt aataatgttg ctcataattt aatctcttat
     2761 cctgggtaaa ctgatatctc gggggcttac gccatcccat catgtttatg ttgctgttgt
     2821 gcgtgttccg ttttgaactc cgccagcaac cttgcttgct cttcagtcag agccaggttt
     2881 tccagaagtt caggtcttct aagccaggta cggcccagcg actgtttcaa acgccagcga
     2941 cgtatctcgg catggttgcc cgacagtaac actggcggaa cttccatccc ttctaacacc
     3001 tcaggccgcg tatagtgcgg gcaatccagc aatccttcag caaaggaatc ttccgttgcc
     3061 gaggcttcat gtcccagtac tcccggaata aaccgggaaa cggagtcaat cagcgtcatt
     3121 gctggtaact caccaccact gagaacgtaa tcgccgattg accattcttc gtcaatttcg
     3181 gtttggatca cgcgctcatc tataccttcg tagcgaccgc acaccagaat caatttttga
     3241 ttcgttgcca gttcgctgac gcccgcttga tcaagcttgc gtccctgtgg tgacagataa
     3301 atcacctttg cgccttcacc cgccgcggct tttgctgcat gaatggcgtc ccgcaagggt
     3361 tgcaccatca ttaacatccc cggtccgccg ccgtaaggac gatcgtccac ggtacggtgc
     3421 cggtcatgcg tgaagtcgcg aggactccaa ctctggatgc tcagcaggcc atttttaact
     3481 gcccggccag ttaccccgta atcggtaatt gcgcggaaca tttcaggaaa caggctaatt
     3541 atgccaatcc acatagcgcc gtcttttacc gtttatccgg tggtttaaaa accaggatcc
     3601 caatctactt cgattgaacg agtagtgaga tcgactttct tgataacctg cccatcgagg
     3661 aacggtacga gacgttcctt gataccaaac gcatctttca ggtttgcctt aatgacgaga
     3721 acgtcattag atccggtttc catcatatcg acgactttac cgagatcgta gccttcagtg
     3781 gttactacct ggcagcccat caggtctttc cagtagtagt ccccctcttc aagctgaggc
     3841 agctgcgatg aatccacgac aatttcacaa ttcgtcagca ggttcgccgc atcacgatcg
     3901 tcaacgcctt tcagcttgat gatcatgtcc tgattgtggt gcttccagct ttccagctgg
     3961 acttgctgcc actgacccgc cttctggata aaccagggct gatagtcaaa aatgctttcg
     4021 gcgtcttcgg tggaagaaaa cactctgagc cacccacgaa taccgtaaga cgaacccatt
     4081 tttcccaaaa cgatgggatc aacaggtgct tgcgcggtga gttgtttgct catcatgacc
     4141 accgtgacag attaagctgc tttgtttact tctttgatca gcgcagcaac gcgatcagaa
     4201 atagttgcgc cctggccaac ccagtgagcg atgcgatcca gatccaggcg agtgccttct
     4261 tctttttcgc tagcgattgg gttgaagaaa ccaacgcgct cgatgaagcg accgttgcgt
     4321 gcattacggc tgtcagcgac aacaacctgg tagaacggac gctttttagc gccgtgacgt
     4381 gctaaacgaa tagttaccat aacatcctct tgtgtgaata aaacaaccgg accccatcga
     4441 ggaacggagt ccggtgtcat attaaaagcc cgaaaatttt actcattttt gcgggaattg
     4501 caatcaacag ttgctaactc tgctgtaaaa ggccgtcggc ggtgcagcca gtttggtgcc
     4561 ggagtgcgcg cagtcaccgg agcgtacacg cagtacgtga ggatgacgag cacatcccgg
     4621 tgccaaaatg gcaaacaagc caggccgatt agcgaccagg gaagcctggg ggcatcatac
     4681 ccttcatgct tctcatcatc ttcgccattc cgcctttctt cattttcttc atcatgcgct
     4741 gcatgtcgtc gaactgcttc agaaggcggt tcacgtcctg cacctgcata ccgcaaccgg
     4801 cagcgatacg acgcttacgc gaacctttga tgatttctgg cttagcgcgc tcttttatcg
     4861 tcatcgagtt gatgatggct tccatacgca ccagcacttt atcgtccatc tgcgacttaa
     4921 cattatccgg gatctgcccc atgcccggca gcttgcccat caggctagcc atgccgccca
     4981 tatttttcat ctggcgcagt tgctcaagaa aatcgttgag atcgaagccg tcgccttttt
     5041 tcagcttgct ggctaatttc tctgcctgcg cgcggtcaac tttgctttcg atatcttcga
     5101 tcagcgacag tacgtcgccc atgccgagaa tacgcgacgc gatgcggtcc ggatggaacg
     5161 gctccagcgc ctcagttttc tcgccaacac cgagaaactt gatcggtttg ccagtgatgt
     5221 gacgaataga gagcgccgca ccgccgcggg catcgccgtc cactttggtc aacactacgc
     5281 cggtaagcgg taacgcttca ttgaatgctt ttgccgtatt ggccgcatcc tgaccggtca
     5341 tggcgtcaac cacaaacagg gtttcaaccg ggttaatcga cgcatggact tgtttgatct
     5401 cgtccatcat cgcttcgtca acgtgcagac gaccagcggt atccaccagc agcacgtcgt
     5461 agaatttcag tttggcttct ttcagcgccg cgttaacgat atctaccggc ttctgaccaa
     5521 catcagaagg gaagaaatca acgcccacct gctctgccag cgtctcaagc tgtttgattg
     5581 ccgccgggcg ataaacgtcg gcagaaacca ccagcacttt cttcttgtgc ttctcgcgca
     5641 ggaacttacc gagtttacca acgctggttg ttttaccggc accttgcagg cccgccatca
     5701 gtacgaccgc aggcggttgc gcagccaggt tcagggtctg gttctcttcg cccatcgccg
     5761 caaccagttc gttacggact attttgacga actcctgccc cggcgtcagg ctcttattaa
     5821 cttcatgacc aaccgctttc tcttttacgc gattgataaa ctcacgcact accggcagag
     5881 cgacgtccgc ctccagcagc gccatgcgca cttcgcgcag cgtatctttt acgttgtctt
     5941 cagtgaggcg tccacggcca ctgatattgc gcagcgtgcg cgacaaacga tcggttaaat
     6001 tatcaaacat tgtctctcgc ctggggtgga aacggttggc cgcaatcgcg acacatcatc
     6061 agtattttgc cgcagtataa catgaaggcg tctttgttgt tatgcaacgg ttggagcagc
     6121 gttcacctga cgctatactg cttctctttc ttattgctca aactgtcgac attactatgc
     6181 ccgtttttgc tctgctcgcg cttgtcgcct actccgtcag tcttgcgctg attgttcccg
     6241 gtctgctgca aaaaaacggc ggctggcggc gcatggctat tatttctgcg gtcattgcgc
     6301 tggtctgcca cgcaatcgct ctggaagccc gcatcctgcc cgacggtgat agcggacaaa
     6361 acctcagcct gctgaacgtt ggttcattgg tcagtttgat gatctgtacg gtaatgacca
     6421 ttgtggcttc tcgcaatcgt ggctggttgc tgctacccat tgtctatgcc tttgcgctta
     6481 tcaacctggc gctggcgacc ttcatgccca atgaatacat cacccatctg gaagcaacgc
     6541 ctgggatgct ggtgcacatt ggcttatcgc tcttttccta tgccacgctg ataattgccg
     6601 ccctgtacgc gctacaactg gcgtggattg attaccaact gaagaacaag aagctggcgt
     6661 ttaaccaaga aatgccgcca ttgatgagta tcgagcgtaa aatgttccac atcacgcaga
     6721 ttggcgtggt gctactaacg ctcacgcttt gcactggcct gttctacatg cacaacttat
     6781 ttagcatgga aaatatcgac aaggccgtgc tctctatcgt ggcgtggttt gtctatattg
     6841 tgctgctgtg gggacattat catgaaggat ggcgtggacg ccgcgtcgtc tggtttaacg
     6901 ttgcgggtgc agtcatcctg acactggcct acttcggcag ccgaattgtc cagcagttaa
     6961 tcagctaaac ccagaaaagg agtttcccct ggaacacatt tctactacta cgttgatcat
     7021 tattctgatc atcatggtgg tcatttcagc ctatttttcc gggtccgaaa ccggaatgat
     7081 gaccctcaac cgctatcgtc tgcgacatat ggcgaaacag ggtaatcgct cggccaaacg
     7141 cgtcgaaaaa ttgctgcgta agccagaccg cctgataagc ctggtgttaa tcggcaataa
     7201 cctggtcaat attcttgcct ccgcgctcgg cactattgtt gggatgcgtt tgtacggcga
     7261 tgcgggcgtg gcaattgcga ctggtgtgct gacttttgtc gtactggttt ttgctgaggt
     7321 attgccgaaa accattgccg cgctgtaccc ggaaaaagtc gcttatccga gtagttttct
     7381 gctggctccg ctgcaaattt tgatgatgcc gctggtctgg ttgctgaatg ctatcacccg
     7441 tatgctgatg cgcatgatgg gtatcaaaac cgatatcgtg gttagcggct ctttgagcaa
     7501 agaagagttg cgcactatcg tgcacgaatc acgctcacaa atttcccgtc gcaatcagga
     7561 tatgctactg tctgtgctcg atctggaaaa gatgaccgtt gatgacatca tggtgccgcg
     7621 cagtgaaatt atcggtattg atatcaacga tgactggaaa tcgattctgc gccaactctc
     7681 ccactcacct cacgggcgca tcgtgctcta ccgtgattcg ctggacgacg ccatcagtat
     7741 gctgcgagta cgtgaagcct ggcggttgat gtcggagaaa aaagagttca ccaaagaaac
     7801 catgctgcgc gccgcggacg agatctattt tgtgccggaa ggtacgccgc tcagcacgca
     7861 gttggtaaag tttcagcgca acaaaaagaa agtcggcctg gtcgtcaacg agtatggaga
     7921 cattcagggg ctggtgacgg ttgaagatat tctggaagag attgtcggcg atttcactac
     7981 gtcgatgtcg ccaacacttg ccgaagaggt cacgccgcaa aacgacggtt cggtgattat
     8041 cgatggcacc gccaacgtgc gggaaatcaa caaagccttt aactggcatc taccggaaga
     8101 tgatgcccgc acggttaatg gcgtcattct tgaggcactg gaagagatcc ctgtcgcagg
     8161 cacccgcgtg cgtattggcg agtacgatat agatattctc gacgtacagg acaatatgat
     8221 taagcaggta aaagtttttc ctgtgaaacc gctgcgcgag agtgtggcgg agtaacgaaa
     8281 acggccctgc attcgcatgc agggccgtga attattacga aaacagaaat tacgcttttg
     8341 ctttcgctac agttaccatc gccgcacgaa tcgtacgacc attcagcgta taacccttct
     8401 gcataatgcc cagtacgtta cctggcgcaa cgtcatcaga ttccaccatt gcgatagcct
     8461 gatgcacatt cgggtccagc ggcacgttag tttcggcgat cacttcaaca ccaaacttac
     8521 gcacaacatc cagcatcgac ttcagcgtca gctcaatgcc ttcaaccatc gcagacatat
     8581 ccgggttagc tttatcagcc acttccagcg cacgatccag gctatcaatc accggcagca
     8641 attcgttgat gaatttctcc agcgcgaatt tgtgggcttt ttcaatatcc agttcagtac
     8701 gtcgacgcag gttttccatt tcggctttta cacgcaaaat gccgtcacgt tcacgggtct
     8761 gggcttcagc cagctgagct tcgagattcg caattttttc atcgcgcgga tccacctgct
     8821 cagcagaagc ttctggctca actgcctcaa tctcttcgtg ctgatccatg ataatttctt
     8881 ccggggcttg cccctcaggc gttttctgtt ctttactact catgaatttc tccgcgtttt
     8941 tttcgcattc atctcgctaa cttcgcttat tatggggatc agtttcaggg tttcaaggga
     9001 agcactcaca ttgtcatcaa tcttcgcaac aaggacctcg gaaaaatgaa taatcatttc
     9061 aagtgtattg gcattgtggg acacccacgg caccccactg cactgacaac acatgaaatg
     9121 ctctaccgct ggctgtgcac aaaaggttac gaggtcatcg ttgagcaaca aatcgctcac
     9181 gaactgcaac tgaagaatgt gaaaactggc acgctcgcgg agattgggca actagctgat
     9241 ctcgcggtag tcgttggtgg cgacggtaat atgctgggcg cggcacgcac actcgcccgt
     9301 tacgatatta aagttattgg aatcaaccgt ggcaacctgg gtttcctgac tgaccttgac
     9361 cccgataacg cccagcaaca gttagccgat gtgctggaag gccactacat cagcgagaaa
     9421 cgttttttgc tggaagcgca agtctgtcag caagattgcc agaaacgcat cagcaccgcg
     9481 ataaatgaag tggtgcttca tccaggcaaa gtggcgcata tgattgagtt cgaagtgtat
     9541 atcgacgaga tctttgcgtt ttctcagcga tctgatggac taattatttc gacgccaaca
     9601 ggctccaccg cctattccct ctctgcaggc ggtcctattc tgaccccctc tctggatgcg
     9661 attaccctgg tgcccatgtt cccgcatacg ttgtcagcac gaccactggt cataaacagc
     9721 agcagcacga tccgtctgcg tttttcgcat cgccgtaacg acctggaaat cagttgcgac
     9781 agccagatag cactgccgat tcaggaaggt gaagatgtcc tgattcgtcg ctgtgattac
     9841 catctgaatc tgattcatcc gaaagattac agttatttca acacattaag caccaagctc
     9901 ggctggtcaa aaaaattatt ctaattttac gccagcctct ttactgtata taaaaccagt
     9961 ttatactgta cacaataaca gtaatggttt ttcatacagg aaaacgacta tgttggcaca
    10021 actgaccatc agcaactttg ctatcgttcg tgagcttgag attgattttc atagcggcat
    10081 gaccgtaata actggcgaga ccggcgcggg taaatctatt gcaatagatg ccctcggtct
    10141 ttgtctcggt ggtcgcgctg aagccgacat ggtgcgtacc ggcgctgctc gcgctgacct
    10201 gtgcgcccgt ttttctctga aagatacgcc agcggctctg cgctggctgg aagaaaatca
    10261 gcttgaagac gggcatgaat gtttgcttcg tcgagtgatc agcagcgatg gtcgctcccg
    10321 tggtttcatc aacggtacag ctgttcctct gtcacaactg cgcgaactgg gtcagttgct
    10381 gattcagatc catggtcagc acgctcatca attactcacc aaacctgagc accaaaaatt
    10441 cctgcttgat ggctatgcca atgaaacctc tctactgcag gaaatgaccg cacgttatca
    10501 gttgtggcat caaagctgcc gtgacctcgc gcatcatcaa cagttaagtc aggaacgcgc
    10561 cgcccgtgcg gaactgctgc aataccaatt aaaagaactt aacgaattta atccgcagcc
    10621 cggagagttt gaacaaatcg acgaagagta caaacgtctg gcgaacagcg gtcaattgct
    10681 gaccaccagc cagaatgcat tggcattaat ggccgacggt gaagacgcaa acctgcaaag
    10741 tcagctttac acggctaaac aactggtgag cgaattgatt ggcatggaca gcaaactgtc
    10801 cggcgtactt gatatgctgg aagaagctac catccagatt gctgaagcca gcgatgaact
    10861 gcgccactac tgcgatcgtc tggatctcga tcccaaccga ctatttgaac ttgaacagcg
    10921 catctcaaaa cagatttcgc tggcacgtaa acatcacgtc agccctgagg cattgccaca
    10981 gtattaccag tcgctactgg aagaacagca gcaactggac gatcaggccg actcacaaga
    11041 aacgcttgcg ctggcggtaa cgaaacatca tcagcaggca ctggaaatcg cgcgcgcatt
    11101 acaccaacaa cgccagcaat atgcagaaga acttgcacag ctgatcaccg acagtatgca
    11161 tgcgctctca atgccgcatg ggcagtttac gatcgatgtt aaatttgacg agcatcacct
    11221 gggcgctgac ggtgccgatc gtattgagtt tcgggtaacc accaacccag gtcagccaat
    11281 gcagcctatt gccaaagtcg catccggtgg tgaattgtcc cgcatcgcac tggcaatcca
    11341 ggtcatcacg gcgcgtaaaa tggaaacccc ggcactgatt tttgatgaag tggatgtagg
    11401 gattagcggt ccaacagcgg cagttgtcgg caaactgctg cgtcaactcg gcgaatcaac
    11461 tcaggtgatg tgtgttaccc acctgccaca agtcgcggga tgtggtcatc aacactattt
    11521 tgtcagcaaa gaaaccgatg gtgcgatgac agaaacgcat atgcaatccc tgaataaaaa
    11581 agcgcggtta caagagctgg cgcgcctgct tggtggcagt gaagtcacac gtaatacact
    11641 ggcgaatgcg aaagaactgc ttgcagcgta aacttttttc ctgcttcacg gtcagagtaa
    11701 acagcaaaac gccgtaagac cggaaagcaa aaggttttaa agtgatgaaa ggtctattat
    11761 catcggcata ttacagatga gccacgtact gctcgggccc gaaaaggaat caaatcacta
    11821 tgcgctgtaa aacgctgact gctgcagcag cagtactatt gatgttgacc gcaggctgtt
    11881 ccactctgga gcgagtggtt taccgtcctg acatcaacca ggggaactat ctgaccgcta
    11941 acgacgtatc caaaatacgt gttggcatga cgcaacaaca agttgcgtac gcattgggta
    12001 caccgctgat gtccgatcca tttggtacga atacctggtt ctatgtcttc cgccagcaac
    12061 caggtcatga aggtgtaacg cagcaaacgc tgacgctgac ctttaacagt agcggtgtgt
    12121 tgaccaatat tgataacaaa cctgcgctga gtggtaacta ataataaagt tgctctcaaa
    12181 gacgttaaaa aaggtgctca atgagcacct tttttctgtc tgttatttat tcgctgattt
    12241 ttctgctcgt tgcctgcgaa gctctttcgg atcggcaatg agagggcgat aaatctccac
    12301 ccgatcgcca tcatgcacac tatcgcttag ttttgccgga cggctgtaaa tgccgacttt
    12361 gtttttagtt aaatcgatat cggtacgcaa ttccagcaag ccactggcgc gaatagcttc
    12421 ttcaaccgtc gcgccctcct gcagcgtcac gcgctgcagg tactgcttct caggtagcgc
    12481 ataagccacc tcaacggcaa ttttacctgg cactgtaaac ctctttcgca cgaaccgtaa
    12541 aagcctggac catattagac gccagctctt taaacacgcg accaaaggcg agttcaatca
    12601 acttattggt aaactcaaag tcgagatgaa actcgatacg acacgcctcc tggctcagcg
    12661 gcgtaaactt ccatccacca atcaatttct tgaacggccc atccaccaga ttcataagaa
    12721 tactttggtt actggtcaac tggttgcggg tagtaaacgt tttgctgatc ccagccttag
    12781 agacatctac cgccgcagtc atctgcccag gagtggactc cagaatccga cttccggtac
    12841 aacccggcaa aaactgagga taagactgaa cgtcattcac taactgatac atttgctccg
    12901 cgctgtaggg taccagtgcg gtccggctaa tctgaggcat aacaatttcc atcaacaaaa
    12961 atccaacaaa taatatcatt tatcctgctg taaaaaaaac gctatcccgg cgctgggtaa
    13021 catcgggttc atgctaagat agagccttgt cccccgcagg attgatatgg ggtgttttcg
    13081 atttcagatt accgatgatt cacgacgctt atgacgaaga aaaaagcaca taaacctggt
    13141 tcagcgacca tcgcgcttaa caagcgcgcc cgtcacgaat actttatcga agaagagttc
    13201 gaagcgggac ttgccctgca aggctgggag gttaaatccc tgcgcgcagg aaaagccaat
    13261 atcagcgaca gctacgtcct tctgcgtgac ggagaggcat ttctgtttgg cgctaacatc
    13321 acgccaatgg ccgtggcctc cacgcatgtg gtgtgcgatc ctacccgtac ccgcaagtta
    13381 cttctcaacc agcgcgaact ggactcattg tacggtcgcg tcaatcgaga aggctatacc
    13441 gtagtggcgc tctccctgta ctggaaaaat gcctggtgca aagtgaaaat cggcgtcgcc
    13501 aaaggtaaaa aacagcacga taaacgttca gatatcaaag aacgcgaatg gcaggtggat
    13561 aaagcacgta tcatgaaaaa cgcccaccgt taaacctgca ctccaattat tgaccagttc
    13621 ctcaccgcgc ctccctctcc ggcggcgcga atgaacatct tattggctat cacatccgac
    13681 acaaatgttg ccatcccatt gcttaatcga ataaaaatca ggctacatgg gtgctaaatc
    13741 tttaacgata acgccattga ggctggtcat ggcgctcata aatctggtat acttaccttt
    13801 acacattggg gctgattctg gattcgacgg gatttgcgaa acccaaggtg catgccgagg
    13861 ggcggttggc ctcgtaaaaa gccgcaaaaa atagtcgcaa acgacgaaaa ctacgcttta
    13921 gcagcttaat aacctgctta gagccctctc tccctagcct ccgctcttag gacggggatc
    13981 aagagaggtc aaacccaaaa gagatcgcgt ggaagccctg cctggggttg aagcgttaaa
    14041 acttaatcag gctagtttgt tagtggcgtg tccgtccgca gctggcaagc gaatgtaaag
    14101 actgactaag catgtagtac cgaggatgta ggaatttcgg acgcgggttc aactcccgcc
    14161 agctccacca cttttgatag gactgcaatc ggacagcggc aataaaaaca gccacttacg
    14221 gactctgacc agacagagcg cagaccgaga aaagacaaaa aaatgcacgt aaaatgcacg
    14281 tgcactttaa aagaacccca gatctcacgg tctggttttt ttctgtttgt aactaatggt
    14341 aacaaaaaca catcacctct tggcgttcct tctccttgat actgtttatc catacagtta
    14401 aaaataacac tgtatacaaa cacagtatag agagattttt atgcgtattg aaatttgcat
    14461 agccaaagaa aaaatcagca aaatgccaaa aggatctgtt cctgccctac agcaagaaat
    14521 gccgtgacgt gccagtaaac gttatgacga tgtagaagtg atcatcaaat ccaccagcaa
    14581 cgatggcctt tcagttactc tcaccgccga taaagattct gcaaaaactt ttgttcagga
    14641 gacgctgaaa gatacctggg agtctgctga cgagtggttt gttcgctaat taacgagtaa
    14701 aatcagtaac ggctggaaat cattcaatac tcacactatc taaaagttca ccagccaaca
    14761 gcggtaagtt ctttcatacg acgtgccgcg gatcctatta ttctagtatg aaatccctaa
    14821 ccgatttcaa attctccaga ctgctcagat tctcttttat cccacgctgg cgctgataaa
    14881 tctagtcatt gcaatcgacc tgcgctatcg ctgccgcagt tcttccagtt tctgcatcga
    14941 taactccatc ttctgaataa gaaggcaaca gaatagataa ttagtgcttt atattcaaaa
    15001 ggttaatagc aaatttggca tgctttttac tgtctagtaa acagcatgcc tctatccata
    15061 aaatcaaaca gttgcaatct aattttggag aacgtttttt cttctccctt ttagcaaatc
    15121 gctataaaaa acaacaaatg gtataaaaat gagcaatcct aatccatgca tgacggactg
    15181 gagaaagtat tcgcaaaagt taaaatcaac agtctgttga ttttgagata taactctctt
    15241 ttctaatatt gacaccttgg ctttgctggt tgctaaaatt agcttgttga ttagcagtat
    15301 ggatagaact tacactgtta tttacttcta aaaatggaat ttatcaatgc ttcctacatc
    15361 gcaattacga ccgtccggga cattctactc caattccgca gaaacatcag caggcatcaa
    15421 aagcgaaatc acaccaactc agatagaaga agcgcgggcc attggtcgtt tatatatcaa
    15481 agattgtgat attgagtatc tgccacagtt accaaacgaa ataacatcag ttacaatcga
    15541 aaattgcaat aacctgacca cccttacagg attaccggtt aatacacaaa acctgtccgt
    15601 cattaactgt gaaaaattac aaatcacaga cataccatcg acagtaaaaa atctacatat
    15661 tgaattaact gattcaccat ttatacacat catatctgaa ggcatcgaat gcctgacggt
    15721 ttgccactgc catatatctg gagtgccaga gagtgtccgt catcttgaga tcagaggtta
    15781 cgccacagac ggcataaaaa atgttccaaa cgggttatta tctctcagca tcaatcgcta
    15841 caccccagaa aatcaggcca gaattgacag cctgatatca ccgtcactac agacgctatc
    15901 tctgactgga tgtagcaata ttatactgcc ggagaaactc ccggagagtc tgagatctgt
    15961 aaccattcat gtggagcaga aaactacatg gaacatcgta gctgaagggg ttcctgatgg
    16021 actggatctt gatttacaaa atgtactact ctctccgaat gtaattaaag aaaaaaacat
    16081 cacttttcag ggcaacgcac tggatgctgc cttgcatttt cgtccgggag acattgtcta
    16141 tgggctatcc tctcccagag aaaaacttgt caacagcatc aaactggtta acgacttttc
    16201 caaaaaagat atcgtaattc agaatacatt aacaaacgca gtatgggacg ccagaacccc
    16261 ccgtaaatat aagcaagact cgcttatcaa aagagcatta aatgaacatg aaagaggaac
    16321 aaaatttaaa cagtgcttaa aaaatcacaa taattataat gtcatcatgg ccgacctttc
    16381 catatacaat cgagacaagt tatgggcaaa aacaagcaag gcaggcctag agtttcaaac
    16441 attaattcgc aacagaacgg ttatgttttg tgcggatggg cttgtcaatt cactcaaact
    16501 catagctaac aagtcagagg gctatggtca gagtattacc gccagcgaat tacgatggat
    16561 ttatcgtaat aaagacaaca gccaaataat gaaaaacata aaattttatc tgcatggcaa
    16621 agagatacca gcagaaagaa tattagatac accagagtgg aaagactacc gtccaaaata
    16681 ctccggttcc acatataaat attcttaatg atagcaaaaa atatattttc gatataatca
    16741 atgttatgat taaagagtat ttcatcaggg caggtaaaaa cagagtaaat cagcaaaaga
    16801 agctgatctt cagcgatact gacactaact gacggtttaa gcggtcgtat gaagcagcag
    16861 ctttccgacg gactgccatg cggatcgttt accttttggg ctattccgcc cgtcatcaag
    16921 cggctcacga gtactgagtt tatcaggatg atattgctga caatggtaat tcgttgaccg
    16981 atgtatactt cactatatat cggtcaacac cgtactgccg tgcaacatct caaagatgta
    17041 gcatcgtttt taccagagga ggcaggacat agattgtaaa aaaactattt cgatgcattt
    17101 accattgaga tattttcctt cataccaaaa gttctatcaa ttatcgcaag tggttcaaca
    17161 ggtgcggtct tatctataac ccaaccactc tctgtaacac ttcctcgtgt gatatatttt
    17221 gcctttactt taacaactat tagataatgc tcacgactaa accgatcagc aacgctaaga
    17281 tccgatgtgt attcaggcaa tattcttcca atacgtacct gctgttgtgc catatattca
    17341 tctggtgctt ttacatttgg atttgaatca ggaagccctc cagcagtttt atattcaacg
    17401 atattttcag cttgaatacc ggttgtgccg cgacacaata tgatgt
//
LOCUS       DAZGLJ010000095        17398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000095 DAZGLJ010000000
VERSION     DAZGLJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.095"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(53..784)
                     /gene="rlmB"
                     /locus_tag="ACHK49_002683"
     CDS             complement(53..784)
                     /gene="rlmB"
                     /locus_tag="ACHK49_002683"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO9263304.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(964..3405)
                     /gene="rnr"
                     /locus_tag="ACHK49_002684"
     CDS             complement(964..3405)
                     /gene="rnr"
                     /locus_tag="ACHK49_002684"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO9263305.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRSGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3444..3869)
                     /gene="nsrR"
                     /locus_tag="ACHK49_002685"
     CDS             complement(3444..3869)
                     /gene="nsrR"
                     /locus_tag="ACHK49_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO9263306.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4074..5372)
                     /gene="purA"
                     /locus_tag="ACHK49_002686"
     CDS             complement(4074..5372)
                     /gene="purA"
                     /locus_tag="ACHK49_002686"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO9263307.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5476..5673)
                     /gene="yjeT"
                     /locus_tag="ACHK49_002687"
     CDS             complement(5476..5673)
                     /gene="yjeT"
                     /locus_tag="ACHK49_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO9263308.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5755..6759)
                     /gene="hflC"
                     /locus_tag="ACHK49_002688"
     CDS             complement(5755..6759)
                     /gene="hflC"
                     /locus_tag="ACHK49_002688"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO9263309.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6762..8021)
                     /gene="hflK"
                     /locus_tag="ACHK49_002689"
     CDS             complement(6762..8021)
                     /gene="hflK"
                     /locus_tag="ACHK49_002689"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO9263310.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8107..9387)
                     /gene="hflX"
                     /locus_tag="ACHK49_002690"
     CDS             complement(8107..9387)
                     /gene="hflX"
                     /locus_tag="ACHK49_002690"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO9263311.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTGRLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9464..9772)
                     /gene="hfq"
                     /locus_tag="ACHK49_002691"
     CDS             complement(9464..9772)
                     /gene="hfq"
                     /locus_tag="ACHK49_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO9263312.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9858..10808)
                     /gene="miaA"
                     /locus_tag="ACHK49_002692"
     CDS             complement(9858..10808)
                     /gene="miaA"
                     /locus_tag="ACHK49_002692"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO9263313.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAVEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10801..12648)
                     /gene="mutL"
                     /locus_tag="ACHK49_002693"
     CDS             complement(10801..12648)
                     /gene="mutL"
                     /locus_tag="ACHK49_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO9263314.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12658..13995)
                     /gene="amiB"
                     /locus_tag="ACHK49_002694"
     CDS             complement(12658..13995)
                     /gene="amiB"
                     /locus_tag="ACHK49_002694"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO9263315.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14014..14475)
                     /gene="tsaE"
                     /locus_tag="ACHK49_002695"
     CDS             complement(14014..14475)
                     /gene="tsaE"
                     /locus_tag="ACHK49_002695"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO9263316.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14447..15994)
                     /gene="nnr"
                     /locus_tag="ACHK49_002696"
     CDS             complement(14447..15994)
                     /gene="nnr"
                     /locus_tag="ACHK49_002696"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO9263317.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15993..17132
                     /gene="queG"
                     /locus_tag="ACHK49_002697"
     CDS             15993..17132
                     /gene="queG"
                     /locus_tag="ACHK49_002697"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO9263318.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAAKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17115..17168)
                     /locus_tag="ACHK49_002698"
     CDS             complement(17115..17168)
                     /locus_tag="ACHK49_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263319.1"
                     /translation="MRFYFYSQAVDESGVTW"
BASE COUNT         3943 a         4973 c         4367 g         4115 t
ORIGIN      
        1 tagacaaaaa aaaaccatcc aaatctggat ggcttttcat aattcagacg aactagctgc
       61 gctgacgaac cgcttcaaat aagcaaattc cggttgcaac cgaaacgttc agggaagaaa
      121 cacttcctgc cattgggatg ctgatcaact catcgcaatg ttcacgggtc aggcgacgca
      181 taccttcacc ttccgcgccc atcaccagcg ccaggcgtcc ggtcattttg ctctgataga
      241 gtgtatgatc cgcctcgcct gccgtaccga cgatccagat attctcttcc tgcaacatac
      301 gcatggtgcg cgcaaggtta gtcacccgaa tcagtggaac gctttctgcc gcgccgcagg
      361 ctactttttt cgccgtagcg ttgagctgtg cggagcgatc tttcggcaca atcaccgcgt
      421 gaacgccagc agcgtccgcg ctacgcaggc acgcgccgag gttgtgcgga tcggttacac
      481 cgtcgaggat cagcaggaac ggttgatcga gcgaagcgat cagatccggc agatcgtttt
      541 cctgatactg acgtcctggc ttcacgcggg cgataatgcc ctgatgcacg gcaccgtcgc
      601 ttttctcgtc gagatattgg cggtttgcca actggataac cacgccctga gactcaaggg
      661 cgtgaatcag cggtaacaga cgtttatctt cacggccttt taaaataaag acttcctgaa
      721 aacgttcagg ggcgcgctcc agcagggcct gcactgcgtg gatgccgtaa atcatttcgc
      781 tcattaatgt actcgttgtt tacgttttgc ctgatgcact acgtttatcc ggcctacatg
      841 atctctgcaa tatattgaat ttgcgtgatt atgtaggccg gataaggcgt tcacgccgca
      901 tccggcatga acaaagcgca ttttgtcagc aatctaaccc tcttctttta aagagggtat
      961 tgatcactct gccacttttt tcttcgccgc acgcttcgct ttggtcgctg cggctatttt
     1021 ctgcgttttc gccgatggct ttttcgcttt tctcgcgtct ttcttcgccg ctttcggctt
     1081 cgtttttttc tcaccgcgga aggcgctgtc tggctcaaag tttacctttt taccgacctg
     1141 acgacgcttg ccgccttttt tgcctgcatc gccttttttc gctttctcgc gcgccgtttt
     1201 accgacgttg cgcggtgcgc gttcactgga gatcagacta aagtcgattt tgcgctcgtc
     1261 catattaacc gcttcgactc gaacttccac gcgatcgccc aggcgataag tctggccgct
     1321 ggattccccc atcaggcgtt gccctacctg gtcaaagcga tagtagtcat tgtccagcga
     1381 agagacatgg accagaccat caatgaacaa gtcgtccaga cggacgaaga agccaaagcc
     1441 agtgacgctg gaaattacgc ctttaaagac gttacctacc tggtcgagca tgaagtcaca
     1501 cttcagccag tcagcgacat cgcgcgttgc ttcgtcggca cgacgttccg ccatcgaaca
     1561 gtgctgaccc agttgcaaca tctcttccat cgaataatgg tagccgccgg tttcagtggt
     1621 gttgccctga tgcccctgct ctttcgccag cagatattta atggcgcggt gcagcgtcag
     1681 gtcaggataa cgacgaatcg gcgaagtaaa gtgcgcatag gactgcaatg ccagaccgaa
     1741 gtgaccacgg ttttctggat cgtaaatcgc ctgtttcatc gagcgtagca gcatggtttg
     1801 cagcatttct gcatcaggac ggtcggcaac cgactccagc agttccgcgt agtcacgcgg
     1861 ttccggcttg ttaccacccg gcagctccag ccccagctcc gccagcactg aacggaaaga
     1921 ggtaatcgct tcggtgctcg gcttgtcgtg aatacggaac agtgccggtt ctttcgcttt
     1981 ctcaacgaaa cgcgccgccg agatattcgc cagaatcatg cactcttcaa ttaatttgtg
     2041 cgcgtcgtta cgctgggtct gttcgatacg ttcaatacgg cgttcagcgt tgaaaatgaa
     2101 cttcgcttct tcgctctcaa atgagatccc accgcgttct tcacgggctt tatccagcac
     2161 tttatagagg ttatgcaact cttcgagatg cttaaccagc ggggcgtact gctcgcgcag
     2221 atcctgatcg ccctgcagaa tatgccagac tttggtgtag gtcagacgcg cgtgagagct
     2281 catcaccgct tcgtagaatt tgtagcccgt caggcggcct ttcgacgaaa ccgtcatctc
     2341 gcacaccata cacaggcggt ctacctgcgg gttgagcgaa cacaggccgt tagagagcac
     2401 ttccggcagc atcgggataa cctgcgaagg gaagtacacc gacgtgccac ggttacgcgc
     2461 ttctctgtcc agcggcgttg gcggacgcac atagtagctg acgtcggcaa tcgcgaccca
     2521 taaacgccag ccgccgctgc gttttttctc gcagtaaact gcatcgtcaa agtcacgggc
     2581 gtcttcgcca tcaatggtga ccagcggtaa atcgcgcaga tcaacacggc ccgcttttgc
     2641 ttcttccggc acttcttctt tcagcccggc aacctgttgc tcaacagcct gcggccagat
     2701 gtacggaatt tcatgggtac gcagagcgat atcaaccgcc atgccggtgc ccatattgtc
     2761 gcccagcact tcgacgattt tacccaccgc tttggtgcgg cgagtcggac gctgagtcag
     2821 ttcgactacg accacaaagc ccatccgcgc gcccatgatc tgatcgggcg ggattaagat
     2881 atcgaagctc agacggctat cgtcaggaac cacaaagccg acgcccgctt cagtaaagta
     2941 gcgaccaaca atctggctgg tttttggcac cagtacgcgg acaatacgcg cttcacgacg
     3001 acctttacgg tcagcgccca gcggctgcgc cagcacctga tcgccatgaa tgcaggtttt
     3061 catctgctcg ctggagagat acaaatcatc tttacgccct tcaacccgca gaaagccgta
     3121 gccatcacgg tggccaataa cggtaccttt caccaggtcg aggcgttccg gcagcgcata
     3181 gcactgacga cgagtgaaga ccagttgacc atcgcgctcc atcgcgcgca ggcggcgacg
     3241 caggccttca agctgctctt cgccttcaat gtgcagttct accgccagct catcacggct
     3301 ggccggtttt tcacgtttgg ttaaatgttc gaggataaat tcccgactag ggatgggatt
     3361 cgcgtatttt tcagcttcgc gttcctggaa aggatcttgt gacatctcgg ttcctccgtt
     3421 gtcatctctg atgaagattt tcgtcactcc accagcaata atttataaag cggttgattc
     3481 tcttcaacca aatcggcaag cgtgtagtta tccagttccg taagaaaact ttgcacggcc
     3541 ttagaaagcg cctgtttcaa ccgacaggca ggtgtaatgt ggcaaaactc actgctgcaa
     3601 ttcaccagcg ataagggctc cagctcgcgc accacatcac caatacgtat cgcactcgcc
     3661 ggtttaccca ggcgaatgcc gccatttttt ccacgaacag cagtcacgta gccggcacga
     3721 ctaagttgat tgattatttt gaccatatga ttacgggaga cgccgtagac gtcagtcact
     3781 tcagaaatac tggtcatccg cccttctggc aatgacgcca tgtagatcag cgcacgtaat
     3841 ccgtaatcag tgaaactcgt taactgcaca tcaacctcaa aagggaaatc gggaaaaaat
     3901 acatttatat tgatgataaa ccagccacaa gctgtgtcgc taatttattt cagttcgggg
     3961 aaggaaaaaa gcgaggattt aacactgtgt cggatagcgt aatggcttaa ccgacacagc
     4021 aatgtatcac actgttagcc cggcaggcaa aatatctgcc aggcgtacca gaattacgcg
     4081 tcgaacggat cgcgcagaat catggtttca gtacgatccg gaccggtaga gatgatatcg
     4141 atcggcacac cagtcagctc ttcaatacgc ttgatgtagt tcagcgccgc ctgcggcaga
     4201 ccgctacgat ctttcacgcc gaaggtggat tcagaccagc ccggcatggt ttcgtaaatc
     4261 ggctctacac ctttccagtc gtcagctgcc agcggagtgg tcgtcacttc acgaccatcc
     4321 ggcatacggt aagccacaca gagtttcacc tctttcaggc catccagaac gtccagttta
     4381 gtcaggcaga agccagacag ggagttcagc tgtaccgcac gacgaacggc aacggtgtcc
     4441 agccagccgg tacgacgacg acgccccgta gttgcgccga attcgttacc ctgcttgcag
     4501 aggaactcgc cagtttcatc aaacagttcg gtcgggaacg gacctgcacc tacacgagtt
     4561 gagtaagctt tgaggatacc cagaacataa tcaacataac gcgggcccag gccggaaccg
     4621 gtcgccacgc caccagcagt ggtgttggaa gaagttacgt acggataagt accgtggtcg
     4681 atatccagca gcgtaccctg cgcaccttca aacatgacga aatcgccacg ctgacgcgcc
     4741 tggtcgagca ggtcagaaac gtcaaccacc atagaagtca ggatgtcggc aacagccatc
     4801 gtatcatcca gaactttctg gtaatcaacc gcttcagctt tgtagtagtt aaccaactgg
     4861 aagttgtgat attccatcac ttctttcagt ttttcagcga aggtttcttt gtcgaaaagg
     4921 tcgccaacac gcagaccgcg acgtgctact ttatcttcat aagcaggccc gataccacga
     4981 ccggtggtgc cgatcgcttt cgcgccacgc gctttctcac gcgcgttatc cagcgcaacg
     5041 tgataatcaa ggatcagcgg acatgcttca gacagcagca gacgctcacg aacggggatg
     5101 ccacggtctt ccagttcttt catctctttc atcagcgcgg ccggagacag cacaacaccg
     5161 ttaccgatga tgctggttac attctcgcgg agaatacctg atggaataag atggagaacg
     5221 gttttttcac cgttgattac gagagtatgg cctgcgttgt gaccgccctg gtagcgtaca
     5281 acatatttag cccgttcagt cagaagatcg acgatcttac ctttaccttc gtcaccccat
     5341 tgggtgccca gtacgacgac gttgttaccc atttttcaaa atcaccgttt gcttaaaaat
     5401 ggattctacc atcgcttttt cagagttaca gcactttttg cactcaaaaa tgacctcgat
     5461 cagtctgttt tttgttcagc caatcgtttt cctcaacatg tagtagacca caacgcccgc
     5521 aaccacaagt ccaccgccaa aacgacgtaa aatattatcg ggcaaattgg tcatcgcaga
     5581 gatcatcttc ttccatgcct tcgggtaaag catcggccct aaaccttcca gtaccaaaac
     5641 cagggcaagc gccagccaga ttgtcgaatt cattcattat ccttatagaa aaagaaaacc
     5701 accgacatcc ctgaggatgc ggtggcttta ttgacctgta ccgcagtcgt tatattaacg
     5761 cgttgcggaa gtcggcgtct tcatgtagcg gaagaaatcg ctatccgggc tcatgaccat
     5821 cacgtcctga ttgccagaga agctgttctc ataagcacgc aggctacgga tgaatgcgta
     5881 gaagtccgga tctttactga atgcatcagc aaacagtttg gctgcttcgg catcgccttc
     5941 accacgcatg atgcggccct gacgctcagc ttctgccagc gttctggtca cttcatagtc
     6001 ggcagtcgcg cgcagttttt ccgcttcttc ctgaccttgt gaacggtgac gacgcgctac
     6061 cgcttcacgc tcggcgcgca tacggttgta gatcgcttca gacacttcgg tcggcaggtt
     6121 gatctgcttg atacgcacat cgacaacttc aatacccagc gccgccatac tgttcgggtt
     6181 gatgaccgga actttgccct tcgtctcagc cgttacgcgc tctgccgctt cggcaatggc
     6241 gttatctgct gccggggtag taacttcatc ttctgtaccc gcagaaccgg agttcagcgc
     6301 gtcacgtact tcgagggtca gacgaccacg ggaatcagtg acgatatctt tcacgtccag
     6361 gcgaccaatt tcagaacgca gacggtcaga gaacttacgt ttcaacagca cttccgcttg
     6421 cgaaatgtcg ccaccacccg ttgccaggta gtaacggctg aaatcgctga tgcgccattt
     6481 gatgtaagaa tcgacgatca ggtctttctt ctctttggtc acaaagcggt cggcctggtt
     6541 gtccatggtc tgaatacgtg cgtcgagcat tttcaccgtt tcaatgaacg gtatcttgaa
     6601 atgcagaccc ggctcataaa ccagaggttt gttgtcatcg tcacgcagta ccttaccaaa
     6661 acgcagcgta ataccgcgct caccttcttt gacgacaaag acagacatgt aaagcactac
     6721 cagcacgatg atgataatcg cgataactga cttacgcatc gttattcccc ctgacgctgg
     6781 tagtcgttac gctgcgcgtt ggcgcggcgt tggtccataa tatcgccctg actggtggac
     6841 gacgtgttgc ttgctccact ggttgtggag gaagaggctg gcggcagacg cagcagattg
     6901 ctggcaccgt tatcgctctt cgccgcaggg gcgttaccac ctttcagcat ctggtctaac
     6961 ggcagaacca tcaggtttcc acctttgtcg ttaaccagca ctttgcgggt gttgcccaac
     7021 actttttcca tcgtctcgat atacagacgc tcgcgagtaa tttccggcgc ggctttatat
     7081 tccggcagaa gtttagcaaa gcgcgccact tcaccctgag cttccaggat ggtctgggcc
     7141 ttgtacgcac gcgcctcttc gaggatacgt tgcgcctgac cgttcgcacg cggctgaact
     7201 tcgttggtat acgcttctgc ttcacgaatg tattgctgtt cgttttcacg cgcggcaatc
     7261 gcatcgtcaa acgccgcttt tacttcttcc ggcggacgag cagcctggaa gttgacgtcc
     7321 agcagcgtga tacccatgtc atacggacga atcgtctctt ccagttcgcg ctgagtatcg
     7381 ctacgaatca cggtacgacc ttccgtcaga atgcggtcca tggtgtattt accgataact
     7441 ccacgcaggg cgctgtcggt agcctgacgc aggctgtcat ccgggctggt cacgctatac
     7501 agatattttt ccggattggt gacgcggtac tgcacgttca tctcaacgcg cactacgttc
     7561 tcgtccgacg tcagcatcac accagaagcg gccagttcac gcacggcttc cacgttcacc
     7621 ggtttgactt cgtcgataaa cgtcggtttc cagttcagac ccggctcaac cagatggctg
     7681 aatttaccaa agcgtgttac cacgccgcgt tcggcttctt taatggtata gaaaccactg
     7741 gccgcccaga taatgacaat cgctgccgct gcgatggtaa cgacacgacc gccaagctgc
     7801 gggcgcgggc cttgcgatga actgccaccg ccagatccgg tgcctttacc gccgcccaga
     7861 ccaccgagct ttttgctcag tttgcggaag atatcatcta aatcaggtgg cccttgatcg
     7921 cgaccgcctt tgtttccatt tccctcagag ttgccgccag gtttgctgct tccccacggg
     7981 tcgcggtctt gtccgttatt accgggctga ttccacgcca tgtttgtgct ccatatttgt
     8041 tatgcggtga tccctgttgt ctttcgacgc cagaggatat gactccacgc ttcagacgct
     8101 acgccgttag atcaggtaat cgatcaacgc cggttcttgt ttacagaggc gacgccagtc
     8161 aacgatcggc atacgaactt gcagacttac gctgccgtcc tcctccatcc actctttttc
     8221 tattgcctga agctgataaa aacgacttct cagacgccct tcctgcggtg gcagacgcaa
     8281 tgtatgctgc gccacctcgc cggaaagccg ccccgtcaaa gcctgaaaaa gctgtggtat
     8341 ccccgctccg gtctgtgcgg aaagccagac acggatcggt ttgttctctt cgtcccgatc
     8401 aatacgcggt tcgaaatctt ccagcatatc gatcttgttc atcaccagca gggttgggat
     8461 ctcgtgagcg tcgatctctt caagaaccgt attcaccgct tcgatgtttt cttgtacacg
     8521 cacatccgcc gcatcaatga cgtgcagcag taatgtggct tgccgcgtct cttgtaacgt
     8581 ggctttaaat gccgccacca gatcgtgcgg caggtggcga ataaacccta cggtatctgc
     8641 aagtacggtt tcaccgacat ctgcaacgtc aatacgccgc aacgtcgggt cgagggtggc
     8701 aaacaactgg tctgccgcgt agacccgcgc ttcggtgatg cgattgaaaa gggtagattt
     8761 accggcgttg gtatatccca ccagcgaaac agtaggaacg tcggctttga tacgcgattg
     8821 ccgcccctgc tcacgctgct tttcaactct ttccaggcgt gactgtatct gcacgatgcg
     8881 attacgcaac aaacgacggt cggtttcgag ctgggtttca cccggaccac gcaaacctat
     8941 cccgcctttc tgtctttcaa ggtgggtcca gccacgcacc aggcgcgtag ccagatggcg
     9001 cagctgcgcc agctcaacct gcaacttacc ctcatgggta cgcgcacgtt gggcgaaaat
     9061 atctaaaata aggccggtgc ggtcgataac acgacactcg cacaaacgct ccaggttacg
     9121 ctcttgcgcc gggctcaggg catggtcaaa aagaacgacc gaagcacccg tcgctttgac
     9181 agcttccgca atttcaactg ctttaccttc acctacaaaa tactttgggt gcggcgcttt
     9241 acggctaccg gtaatcacct gcaatgcttc gacaccggcg gaagagacca gagattcaaa
     9301 ctcctggagg tcttccatat ctttgtcttg cgtaaaatag atgtgtacca gtaccgcctg
     9361 ctcaccagca tcataacggt caaacaagcg tataaccctc taaatagatc agcggggaac
     9421 gcaggatcgc tggctccccg tgtaaaaaaa acagcccgaa accttattcg gtttcttcgc
     9481 tgtcctgttg cgcggaagta ttctgcgcgc tgctaccatg atggtagtta ctgctggtac
     9541 cgccaccggc gttgttactg tgatgagaaa ccgggcgaga cgggacaaca gtagaaatcg
     9601 cgtgcttgta aaccatctgg ctgaccgtgt ttttcaacag gatcacgaat tgatcaaaag
     9661 actcgatttg cccttgcagc ttaataccat tcaccaaata aatagaaact ggaacacgtt
     9721 cccgacgcag tgcgttcagg aacggatctt gtaaagattg ccccttagcc attctctctt
     9781 ttccttatat gcttatttgt actttgaacc tttcgattct gaaaaaattg cgcacgatac
     9841 gtctcaattg tacacattca gcctgcgata gcaccaacaa cctgtaatac ttcgtcgcgc
     9901 gcctgttctg gtttttcact gtcaagccag tgaacccctt cccaaccacg cagccaggtt
     9961 atctgccgct tcgccaactg tctcgtggcg caaacacctc gataaaccat ttcatcgtat
    10021 gagatttcgc cttcaaggta agaccacatc tggcgataac ccacgcaacg aatggaaggc
    10081 aagtccgtat gcaaatctcc tcgggcaaaa agcgcccgga cttctgcttc aaaacctgaa
    10141 gccaacatct gatgaaaacg ctgctcaatg cgttgatgga gcagttcacg gctcgccggg
    10201 gcgatggcga actgatgcac ctgatacggt agagcgtctc ctgacgtttg cgtcagttcc
    10261 gttaaagttt tacccgaaat gaaaaaaact tccagtgccc gggaaagcct ttgtggatca
    10321 tttggatgaa tccttgctgc cgcaaccgga tctacctcct gaagttgacg atgcaatgac
    10381 tcccaacctt gctctaccgc ctgttgctca attctggccc gcacttccgg gtctgccgac
    10441 ggtagcggcg acaacccttc cagcaatgcc ttgaaataca acatcgtacc gcccactaac
    10501 agtgggatcc gccccgccgc ggtgatatcg gccatttccg ccagcgcatc gcggcgaaaa
    10561 tcagcagccg agtaagcctg cgacggatcg cgaatatcca gcaatcggtg cggcgcggcg
    10621 agtaactctt cagcgttcgg cttcgccgtc ccgatatcca tccctttgta aataagggca
    10681 gaatcaacgc ttatcaactc tactggtaaa attttacgca gctcaatggc taacgccgtt
    10741 ttaccggagg ccgtcggccc catcaaaaaa atcgccttag gcaggctcgc cttactgata
    10801 tcactcatct ttcagggctt ttatcgccgg atgtaaatca acagattgta acagaccacc
    10861 cggcggcgtt ttcacaagtt gcggacataa ccgttccacg tccgccagca gggttatggc
    10921 ctgtgccatt gaccactgcg catgttcgct catcagattt cgtgcaatcc actgcgcaat
    10981 attgccaggt tcgaatacgg actgcttcgc caggtagcct atcagttcag gaatcaagat
    11041 ttgtaaattt tgttggcgta agggtaaagg cacggccctg atggtcacat gctgtgcatc
    11101 tgactggaaa tcaataccca attccgccag ggcagactgc gctttttcta atgccgattt
    11161 ttcttcgcca gaaactttta gccgcaacgg aatcagcaac ggctgggcgc aaactggcgc
    11221 ttcacccggc gtcagttgcg cctgacgcag ccaacgttct gccactggca aggctaaaag
    11281 tgaaatgttg ccatcgcgct ccagcaacgc acagtcggaa tggacgatag tcagtacccg
    11341 accaaaactc tgactgttcg ccgcaagtgc aggttcctgc ggttccggcg cttttggttt
    11401 ttgcatcggc gcgggcgttt gcaaaagctg gcgatacact tcaccttgct gtttctggta
    11461 gcctggctgc gcattcggcc agggggcagc cggacgactg cctgaggcgg gcgcaggaga
    11521 gtagcgcgga gctaccggct cacgagctgc cggttctgca aagtgattgc gccccgccgc
    11581 cacgcggttt tccggaatgg gacgcggtgc aggttggggt tcatcgtcca gcggtagcgg
    11641 cgtttccagt tgctgttgta gcacgctcag cacgccctga tagataaaat catgcaccag
    11701 acgcgactga tggaaacgca cttcgtgttt ggcggggtgt acgttgacgt ccacctgatg
    11761 cgggtcgatc tccagataca acacaaacgc cggttgttga tcggccccca gtttgtcttc
    11821 gcaggcctgg cggatcgcat gattgatcag gcgatcgcgc atcatgcgac cattcacgta
    11881 gcaatactga atttccgcca gtgcgggcgt agtgtgattt ggatcggcca cccagccgcg
    11941 cagcgtgaga tcgccgtgtt gccattcaat cgccagcgct tgttcgagaa aagcggtgcc
    12001 acaaatcgcg cctaagcgcc gttctttttg cccgccttcc ggcactgcgc ggtactgacg
    12061 cacaatttta ccgttatgcg acaggttgat cgtgacgtcg aaacgcgcca gcgcaatgcg
    12121 gcggatgatt tcatcaatat ggctaaattc ggttttctcg gtgcgcagga atttgcgccg
    12181 cgccggggtg ttgtagaaca gatccagcac ctccagcgtc gtccccacag gatgcgccgc
    12241 cggttttacc gtcacgttca tatcgcgccc ttcggcatag gcctgccagg cttcctgctg
    12301 ttctgcggtg cgtgaagtga gcgtcaggcg ggaaaccgaa ctgatactcg ccagcgcctc
    12361 accgcgaaag cccaggctga taatggcttc gagatcgtcc agagaggcga ttttactggt
    12421 ggcatgacga gccagcgcca gcgccagctc atcttttttg ataccgcagc cgttatcacg
    12481 aatgcggata agtttcgccc caccgcgttc gatatcaata tcgatacgcg tcgcacctgc
    12541 atcgaggctg ttttccacca gttctttgac taccgacgca ggtcgctcga ccacctcacc
    12601 tgcggcaatc tggttcgcca gttgtggcgg taagacctga attggcatca atcgtcctta
    12661 gtttggcagc gtgcgatctg gcgtcgtcac cgtactggca gtttgtgccg ttgccccctg
    12721 cggcgcagat tgcatcggat gcgcaaggaa ataattgcgc aggcctttgt aaatggcttc
    12781 tgccagctgt tgttggtaat cgtcgctcgc cagcaaacgt tcttcgctgt tgttgctgat
    12841 aaaaccggtt tcgaccagta ctgacgggat atccggcgaa cgcagaacgc cgagactcgc
    12901 gtgttctggt cggcgtttat gtatttcgcc aatgcgttgc aactgactga tcatactggt
    12961 cgctacatca taccctaccc gttgggaatg accgaactgt aaatccagca ccgcctggct
    13021 taaatagggg tcagactgac tgttcgccag cacatcaccc gccccaccca gcagctccga
    13081 ctgtttctcg tgctgctcca gccagctggc catttcgctg ttggcgcgac ggttagagag
    13141 cacccatacg gaagcgccag tcgcactgcg gttcggtgcg gcatcagcgt gaatcgacac
    13201 gaggaaattg gcgttttgct tacgtgccac atcactgcgc cccatcaccg agataaagta
    13261 atccccgtca cgggttaaaa cgcccttaaa catcggatcg tcattgagca aagtacgcaa
    13321 tttacgcgcg atggcgatgg tgacattttt ctcccgcgta ccaccggggc cgatagcgcc
    13381 agggtcctga ccgccgtgtc cggcatcaat agcgatgata attttatcgc cagtgttagc
    13441 cgtcgcgcgc gctgccggac gcgttaccgt attactgctg ataacacccg tagtgcggtt
    13501 actttccgtt ttaaacggat tgcgcgccgg ttcgctgacg cgcggtgcga caaccgcagg
    13561 cgtttcaacg cgtttcgcaa ccacgggcgg cggaggagga ggtggcggcg catcggcgtt
    13621 aatcgtaaag acgacagtgt aattgctgcc attctgccgc ttcaccgctt cggttttacc
    13681 gttttcggta agatcgacca ccagccgtag cgtttgtgca tctttaggcg ttccagagcg
    13741 aatcgccttc accagattat tgccgctgaa caacaacggc agtccctgaa tcacgcccgt
    13801 ttgtttgata tcgagcgcca cggtgcgttt gctttgatgg ctaaacgcat aatcaggatc
    13861 gccaataaaa ctcaacgtta tccgcgcctg ttgattaccg ttagaaacct gaatatcaga
    13921 gagcgtcgcg gcacccaccg gcgtgcacag cagcagcagc gtcgctacca accaatttct
    13981 gatgcgatac atcatcccgc cacctttcaa aggttaaccg gctaaacgcg ccagcaacaa
    14041 ttcacccgca gaggaaaccg cactcacgcg cgcctcacgg ccttgtgcct gataatcaat
    14101 gtgtatttcg acatccgggt caggaagaac acctgtacct tgttgtggcc actccaccag
    14161 gcagatggca tcgttggcaa aataatcgcg gatccccata aactccagct cctcgggatc
    14221 ggcaaggcgg tacaaatcaa agtgataaac cattaaattg tcgagcgtat agggttcgac
    14281 cagcgtataa gtggggcttt tgacattacc ctgatgaccc agagcttgta aaaagccccg
    14341 gctaaaggtg gttttacctg cgcctaaatc gccatacaga tagattacgg ttgcgccatc
    14401 gcaggctttc gctacccgct cgcccaggtc taatgttgcc tgctcatcag ggagcggaat
    14461 tactcgattc atcatggttt ttatcagtca cttccgggtt aacaatacgc tgtagcgtgg
    14521 aaaagagatc ggttgccagc atcccgcgcg ttccaaaacg cgccgccagt acgtcagctg
    14581 cagcaccgtg cgcgacacag cccgcacagg cggcatcata cggcgacagt ttttgcccaa
    14641 gcaatgcgcc aataatacca gagagcacat cgcccatgcc gccgctcgcc atgcctgcat
    14701 ttccggcatc aataatgcct aaagcgtcag gatgggcggc gaccacggtt ccggcacctt
    14761 tcagcaccgc tacgccgcca taacgttgta ccagacgttt ggcgcaatgt aagcggtcac
    14821 tttcaatttc agcgacggaa cagcctaaca accgtgcggc ctcgccagga tgcggcgtga
    14881 tcacgcgatt gtgacgctta tcgggattga ttgccagcag gttcaatgca tcggcatccc
    14941 acaacatcgg tttgcgaaaa ttctcaactt tttgcagtgc ttttttcccc cactcttgct
    15001 ggcccagacc gggaccaatc accaccacat cggcccattc caggctttcg gtaagagagt
    15061 ccatcgtcag ttcatgcacc atcaattccg gtcgtgcagt cagcagcggc gcaatgtttt
    15121 cactgcgggt cagtactcgg actaaaccag caccagcacg tagcgccgct tcccccgtca
    15181 tacgaatagc ccccgccgtg ccgtgatcgc caccgataat taccagccgc ccgtgatcgc
    15241 ctttatgcga agtcgggcga cgcggtttta gccagtgaga aagttgttct gccgaaaacc
    15301 gctgaatttt cgtctcctga cctgccagcc aactatccag ccccagtgag tcaaaatgca
    15361 gttgtccggt aacatcccgc gcttttccag tgagcaagcc tggtttcagc gcaataaaag
    15421 tgatggtgtg atcggcgttg atcactgcgc ctggcgtagc gccggtttca gccagcaggc
    15481 cggaagggat atcaaccgcc gcaatcggcg caggatggga attagcgtgg tcgattaact
    15541 ggctaatgga ttcgcggggc gcttgctgca agccggtacc gagcagcgca tcaacaatca
    15601 gatctaccga ttcgggccag acaatattcg aagcatggat ctcgcctccc gcgtttaacc
    15661 atgcttcgcg tgccagcgcg gcctcttccg gcaacggttt gtcgctctcc tgggccaaca
    15721 acgtgacctc aatgccgacc gctgtggcca gtcgcgcgac cacgtagcca tcgccgccgt
    15781 tattaccatg accgcacagc accagccagt ggcgggcgtc aggatacgcc gaacgacaca
    15841 cctggaatgc ggcttcgcca gcgcgaagca tcagctcata gagtgtaagc cccagcgcat
    15901 ctgccgcctc acgttctccg cggcggatat cgtcggcgtg ccagacggtg tgtggtatac
    15961 ttacggggtt tttcttcatt gtatggtccg tcatgtcaga gcccctcgat ctcaatcagt
    16021 tagcgcaaaa aattaaacag tgggggctgg aactgggctt tcagcaggta ggtattaccg
    16081 ataccgatct cagcgagtcc gagcccaaac tgcaagcatg gctggacaaa caataccacg
    16141 gcgaaatgga ctggatggca cgtcacggta tgctgcgcgc tcgcccccat gagttattac
    16201 ccggtacgct gcgcgtgatc agcgtgcgga tgaattacct tcctgctaac gccgcatttg
    16261 ccagcacgct gaaaaacccc aaactcggct atgttagccg ttatgcgctg ggccgtgact
    16321 atcacaaact tctgcgcaac cgactcaaaa agctgggcga gatgattcag caacattgtg
    16381 tttcgctgaa ttttagaccg tttgtcgatt ctgcgcctat tctcgagcgc ccgttagctg
    16441 caaaagctgg gctcggctgg acaggtaagc actcacttat cctcaatcgc gaggccggtt
    16501 ctttcttctt tttaggcgaa ttgctggtcg atattccgct gcccgtggat caaccagtcg
    16561 aggaaggatg cggtaaatgc atagcctgta tgacgatttg cccgaccggt gccatcgtcg
    16621 agccatatac cgtcgatgct cgccgctgta tctcttatct caccatcgaa ctggaagggg
    16681 cgatcccgga agagttgcga ccgttaatgg gaaaccgtat ttacggttgc gatgactgcc
    16741 agcttatctg cccgtggaat cgctattcgc aactcactac agaagacgat ttcagcccgc
    16801 gtaagccgct acacgcaccg gaactcattg agttattcgc ctggagcgaa gagaagtttt
    16861 taaaagtcac ggaaggatcg gcgattcgcc gtataggtca cctgcgctgg ctgcgtaata
    16921 tcgccgtggc attaggcaat gccccctggg atgaaacgat tctggcggcg ctggagagtc
    16981 gcaaaggtga gcacccactt cttgatgagc acatagcgtg ggcgatggcg cagcaaatcg
    17041 agaggcgaaa tgcgtgcata gtcgaagtgc aattgccgaa aaaacagcgt ctggttcggg
    17101 tgattgaaaa agggttacca cgtgacgcct gattcatcca cagcttgtga ataaaaataa
    17161 aaacgcattg caattcaaga cgaataaaat cgtcaagtga tcgactcgac aaaacgaaat
    17221 cttaattttt aacttatcta tcaataagtt aaaaaaatac tattcaccgt gcgaagtttc
    17281 tttgaaatta agactacagc ctgaggctgt ggataactct gtttacaaga gtttgtcaga
    17341 gacaacaaaa accagcaata acgcatcgcg tcgctgtgga tattttattg agagaaga
//
LOCUS       DAZGLJ010000096        17293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000096 DAZGLJ010000000
VERSION     DAZGLJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.096"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1333)
                     /gene="djlB"
                     /locus_tag="ACHK49_002699"
     CDS             complement(<1..1333)
                     /gene="djlB"
                     /locus_tag="ACHK49_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO9263320.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQANQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVD"
     gene            complement(1330..2037)
                     /gene="ybeR"
                     /locus_tag="ACHK49_002700"
     CDS             complement(1330..2037)
                     /gene="ybeR"
                     /locus_tag="ACHK49_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO9263321.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            2201..3178
                     /gene="ybeQ"
                     /locus_tag="ACHK49_002701"
     CDS             2201..3178
                     /gene="ybeQ"
                     /locus_tag="ACHK49_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFO9263322.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3248..3730)
                     /gene="ybeL"
                     /locus_tag="ACHK49_002702"
     CDS             complement(3248..3730)
                     /gene="ybeL"
                     /locus_tag="ACHK49_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO9263323.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            3965..6547
                     /gene="leuS"
                     /locus_tag="ACHK49_002703"
     CDS             3965..6547
                     /gene="leuS"
                     /locus_tag="ACHK49_002703"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO9263324.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            6562..7143
                     /gene="lptE"
                     /locus_tag="ACHK49_002704"
     CDS             6562..7143
                     /gene="lptE"
                     /locus_tag="ACHK49_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO9263325.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7143..8174
                     /gene="holA"
                     /locus_tag="ACHK49_002705"
     CDS             7143..8174
                     /gene="holA"
                     /locus_tag="ACHK49_002705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO9263326.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8176..8817
                     /gene="nadD"
                     /locus_tag="ACHK49_002706"
     CDS             8176..8817
                     /gene="nadD"
                     /locus_tag="ACHK49_002706"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO9263327.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            8841..9452
                     /gene="cobC"
                     /locus_tag="ACHK49_002707"
     CDS             8841..9452
                     /gene="cobC"
                     /locus_tag="ACHK49_002707"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO9263328.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARRVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            9712..10029
                     /gene="rsfS"
                     /locus_tag="ACHK49_002708"
     CDS             9712..10029
                     /gene="rsfS"
                     /locus_tag="ACHK49_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO9263329.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10033..10500
                     /gene="rlmH"
                     /locus_tag="ACHK49_002709"
     CDS             10033..10500
                     /gene="rlmH"
                     /locus_tag="ACHK49_002709"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO9263330.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            10531..12432
                     /gene="mrdA"
                     /locus_tag="ACHK49_002710"
     CDS             10531..12432
                     /gene="mrdA"
                     /locus_tag="ACHK49_002710"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO9263331.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12435..13547
                     /gene="mrdB"
                     /locus_tag="ACHK49_002711"
                     /gene_synonym="rodA"
     CDS             12435..13547
                     /gene="mrdB"
                     /locus_tag="ACHK49_002711"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO9263332.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            13558..14646
                     /gene="rlpA"
                     /locus_tag="ACHK49_002712"
     CDS             13558..14646
                     /gene="rlpA"
                     /locus_tag="ACHK49_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO9263333.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            14786..15997
                     /gene="dacA"
                     /locus_tag="ACHK49_002713"
     CDS             14786..15997
                     /gene="dacA"
                     /locus_tag="ACHK49_002713"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO9263334.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16108..16371
                     /gene="ybeD"
                     /locus_tag="ACHK49_002714"
     CDS             16108..16371
                     /gene="ybeD"
                     /locus_tag="ACHK49_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO9263335.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            16472..17113
                     /gene="lipB"
                     /locus_tag="ACHK49_002715"
     CDS             16472..17113
                     /gene="lipB"
                     /locus_tag="ACHK49_002715"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO9263336.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         4344 a         4361 c         4429 g         4159 t
ORIGIN      
        1 cgtcaacggg cattggctgc ggttgcggct ccggttcaac agtttgcttc tcggtaaaat
       61 tcgccgcctg aattaataat cgacgaataa ttaatgcttc cagtggacaa cgatcttctg
      121 catcaaggcg gctcacaatc ggcattaatg attcttcggt tggttggttc gcctgcacta
      181 attgtttggc aactaaatat aatggccata agggatgttc tgtatcacgc gtgagtgaga
      241 tccgcggctt acgtcctttg tgcaaaacca tctcgaccag tgaacgcccc agagcagaga
      301 gtttttcatc gctgagtatt tcgtataaat gaaaacgcgt acgggcgctg tcgccgggga
      361 tataaagtag cggttttccc tgatcgttaa ccagttcacg agcttcaatc atcatcacca
      421 ctaacgggta ataatcgacc cgatgctgac ggcaccattg caatattaaa gcatccgcct
      481 gggtggaggg atactcgcgc cagtagtcac aaagttctgc cagcaagctt tcgccttctc
      541 cacaatagac gcgttgtgcg tattcataat aacgcgcatc ttcattatca gattcagctt
      601 cccgccacga ccgggcgtaa tccaggagtt cgggaattcc caggcgacct gcgctatacc
      661 agcggagtaa atttctgtgc agttttgcat catcggggat aatcactgcg cgatgttgcg
      721 ctaaatacaa tgtcagccat tctggatgcg atgaccaaat ccgagcgaac atttggtaaa
      781 aatcgatggt gtcattttgc acggcaacgg gcagatgcag aatggtctgg aagttaaaaa
      841 cattcccctt cgcaatggca tataaaaagg attccagttc ttcttcgtct atttccccga
      901 cgttttcctc ttgcaaccac cgcaatcttt ctgctaataa caccacgcac tcgaaggata
      961 aatgggcagt gttcatggcg attgtgcaca gcgaccagcg taattcatca atctcttcca
     1021 tcgagcaata atttaattgc tgaatgaatc gctgccaggc ggagggcaga aaacgttcgc
     1081 tatcagaggc aagtaacgca cgaaaggcga gcagaatttc atgttctgct acctcatatt
     1141 cttctggttg ccaaacagat ttagcaggcg actgcgcaat ccgtagcgcc tcctcatacg
     1201 cctgacgaag ttgtttaaaa ccctgcggat cggtttctgg atgaaaggac ggtaacagcg
     1261 ccagataagc gcggcggata atatcgacgt cagtcgtttc ctctatttcg agaatcttcc
     1321 agcaattttt cataggtcgc tcatatcctt aagcgtttcc gggcatgcag aatagtaagc
     1381 caatggttgc caggacaagg tagggtaata cgcatcatca ctggcaaata attgataaaa
     1441 catatcattg ccggaatcgt cgtattcttt acgcgctaag aattcgcgta ataactgcag
     1501 atgatcatct tcgggagact gccaatattg ccgaccaaag gtataaccgg caaagtattg
     1561 ccgccagtta cgataataac gtagagcccg tagatgaatg cgcgactgga tccatagact
     1621 ttcttcttcg ctcaaccagt tattaagtac gcccattcgg cttaaaaaac ccatccggac
     1681 ataatcccag gctttaattc ctccctcacc acagcattcg gcggtgctgg cgacaaattg
     1741 cgcataagcc tgaccttgct catttagttc agcaagatag tcacgccatt cacatggcga
     1801 ataacgaaac cagcggtgat aaaaccctgc caaccgagcg gcatgaccgt tatcgatcat
     1861 tcggtgaatt gtgcgcagca ggtcatcacg ggcatcaatt ccccaggagg aagaaaggtc
     1921 aaaaaagcat gtacctaaat acattttggg tgatgaatgc gaaggtaaac agcattcatt
     1981 gcgtatttcc atcggagtgg aaagtgcaaa caatattttt tgcgactcca tgtccatata
     2041 atttatcctt aaatattcat taaatgaata ttagccgcat tgcgaggctc gtttatttat
     2101 acttttctga cgcgacataa tacgcataaa ataaggctta cgcaaaaatg gaacacataa
     2161 aaatataata tgttttatct gcaaccataa ggaaatgata atgattttca cgtcaagttg
     2221 ctgcgataat ttatcaatag atgagattat cgaacgtgct gaaaaaggcg attgcgaggc
     2281 tcaatatatt gttgggtttt attataatcg cgatagcgca attgattctc cggacgacga
     2341 aaaagccttt tactggctga agctggccgc tgagcaaggt cattgtgaag cacagtattc
     2401 cttagggcaa aagtataccg aggataaaag ccgtcataaa gataatgagc aagccatctt
     2461 ctggctgaaa aaagctgccc tacaaggcca tactttcgct tccaacgccc ttggctggac
     2521 actggatcgt ggagaagccc ccaattataa agaagcggtt gtctggtatc agatagccgc
     2581 ggagagcgga atgtcttatg cgcaaaataa tcttgggtgg atgtacagaa atggcaacgg
     2641 agtcgcaaaa gactatgcgc tggcattttt ctggtacaaa caagctgcat tacaaggcca
     2701 tagtgacgcg caaaacaatc tggccgatct ttatgaagac ggaaaaggcg ttgctcaaaa
     2761 caagacactc gccgcattct ggtatttgaa aagcgcacag cagggtaatc ggcacgccca
     2821 gtttcaaatt gcatgggatt ataacgctgg cgaaggggtg gaccaggact ataagcaagc
     2881 gatgtactgg tatctgaagg ctgccgctca ggaaagcgtc ggcgcttacg tcaacatcgg
     2941 ttatatgtat aaacacggac aaggcgttga gaaagattat caggctgcct ttgaatggtt
     3001 tacgaaagcc gctgaatgca atgacgccac tgcctggtat aacctggcca ttatgtatca
     3061 ctacggagaa ggaagacctg tcgatctccg acaggctctc gacctgtatc gtaaagttca
     3121 gtcatccgga accagagatg tcagtcagga aattcgtgag actgaagatt tactgtagag
     3181 ataaaaacaa gggccgggga atttcattcg gcccatattc tctttattgc tgtgcgaaat
     3241 cagactatta cggctcaaac gggcgtctct ggaactggtc atgaccacat ttcgggcata
     3301 gcgtcagcac ttccggtgtg tagatcggga gatggaagtg acatttctcg cagaccagat
     3361 ttcccagccc gaccacttct ccgctgtgat aaaccccatg atgattgagg tcctggaaaa
     3421 cttcgcgcca ttcaagctgc gttttatcgg tgatatctgc cagctcctgc cataagcttt
     3481 ctttaatcac ccgcataaag acgctgtcag attcttcttt caggctctct tcatagctca
     3541 tggcgaactc ttccaggtca cgtcttacag ctcgcgtcag ctcatcgacc tcggttcgcg
     3601 ttaactcccc tgtttttatt acgcgctcgc gcgcctgttc caccagtgcg tcgatatcac
     3661 gttcgccatt gcgcaggcgt tcgctcagtg acgcgaccag ttcacggtaa tattgagcaa
     3721 ccttgttcat cgtttcatct cccgggtcgt taactgacta taataataga cgttctttgt
     3781 tccttgcttt gctaatacgc ccacaaactc tgtaaattaa tgtatgaata atccgggcat
     3841 catttcaacg tcgagtgcgc gaaaggctgt tttgacgcgg acgtttgggc tatgctatgc
     3901 ggatctgaaa aaccacctca acgctacatt tgtagccgta ttgaaaacag gaccactggc
     3961 tgccatgcaa gagcaatacc gcccggaaga gatagaatcc aaagtacagc ttcattggga
     4021 tgagaagcgc acatttgaag taaccgaaga cgagagcaaa gagaagtatt actgcctgtc
     4081 tatgcttccc tatccttctg gtcgactaca catgggccac gtacgtaact acaccatcgg
     4141 tgacgtgatc gcccgctacc agcgtatgct gggcaaaaac gtcctgcagc cgatcggctg
     4201 ggacgcgttt ggtctgcctg cggaaggcgc ggcggtgaaa aacaacaccg ctccggcacc
     4261 gtggacgtac gacaacatcg cgtatatgaa aaaccagctc aaaatgctgg gctttggtta
     4321 tgactggagc cgcgagctgg caacctgtac gccggaatac taccgttggg aacagaaatt
     4381 cttcaccgag ctgtataaaa aaggcctggt atataagaag acttctgcgg tcaactggtg
     4441 cccgaacgac cagaccgtac tggcgaacga acaagttatc gacggctgct gctggcgctg
     4501 cgataccaaa gttgaacgta aagagatccc gcagtggttt atcaaaatca ctgcttacgc
     4561 tgacgagctg ctcaacgatc tggataaact ggatcactgg ccagacaccg ttaaaaccat
     4621 gcagcgtaac tggatcggtc gttccgaagg cgtggagatc accttcaacg ttaacgacta
     4681 tgacaacacg ctgaccgttt acactacccg cccggacacc tttatgggtt gtacctacct
     4741 ggcggtagct gcgggtcatc cgctggcgca gaaagcggcg gaaaataatc ctgaactggc
     4801 ggcctttatt gacgaatgcc gtaacaccaa agttgccgaa gctgaaatgg cgacgatgga
     4861 gaaaaaaggc gtcgatactg gctttaaagc tgttcaccca ttaacgggcg aagaaattcc
     4921 cgtttgggca gcaaacttcg tattgatgga gtacggcacg ggcgcagtta tggcggttcc
     4981 gggtcacgac cagcgcgact acgagtttgc ctctaaatac ggcctgaaca tcaaaccagt
     5041 tatcctggct gctgacggct ctgagccaga tctttctcag caagccctga ctgaaaaagg
     5101 cgtgctgttc aactctggcg aattcaacgg tcttgaccat gaagcggcct tcaacgccat
     5161 cgccgataaa ctgactgcga tgggcgttgg cgagcgtaaa gtgaactacc gcctgcgcga
     5221 ctggggtgtt tctcgtcagc gttactgggg cgcgccgatt ccgatggtga cgctggaaga
     5281 cggtaccgta atgccgaccc cggacgacca gctgccggtg atcctgccgg aagatgtggt
     5341 gatggacggc attaccagcc cgattaaagc agatccggag tgggcaaaaa ctaccgttaa
     5401 cggtatgcca gcgctgcgtg aaaccgacac tttcgacacc tttatggagt cctcctggta
     5461 ctatgcgcgc tacacttgcc cagagtacaa agaaggtatg ctggattccg aagcggctaa
     5521 ctactggctg ccggtggata tctacattgg tggtatcgaa cacgccatta tgcacctgct
     5581 ctacttccgc ttcttccaca aactgatgcg tgatgcaggc atggtgaact ctgacgaacc
     5641 agcgaaacag ttgctgtgtc agggtatggt gctggcagat gccttctact atgttggcga
     5701 aaacggcgaa cgtaactggg tttccccggt tgatgctatc gttgaacgtg acgagaaagg
     5761 ccgtatcgtg aaagcgaaag atgcggcagg ccatgaactg gtttataccg gcatgagcaa
     5821 aatgtccaag tcgaagaaca acggtatcga cccgcaggtg atggttgaac gttacggcgc
     5881 ggacaccgtt cgtctgttta tgatgtttgc ttctccggct gatatgactc tcgaatggca
     5941 ggaatccggt gtggaagggg ctaaccgctt cctgaaacgt gtctggaaac tggtttacga
     6001 gcacacagca aaaggtgatg ttgcggcact gaacgttgat gcgctgactg aagatcagaa
     6061 agcgctgcgt cgcgatgtgc ataaaacgat cgctaaagtg accgatgata tcggccgtcg
     6121 tcagaccttc aacaccgcaa ttgcggcgat tatggagctg atgaacaaac tggcgaaagc
     6181 accaaccgat ggcgagcagg atcgcgctct gatgcaggaa gcgctgctgg ccgttgtccg
     6241 tatgcttaac ccgttcaccc cgcacatctg cttcacgctg tggcaggaac tgaaaggcga
     6301 aggcgatatc gacaacgcgc cgtggccggt tgctgacgaa aaagcgatgg tggaagactc
     6361 cacgctggtc gtggtgcagg ttaacggtaa agtccgtgcc aaaatcaccg ttccggtgga
     6421 cgcaacggaa gaacaggttc gcgaacgtgc tggccaggaa catctggtag caaaatatct
     6481 tgatggcgtt accgtgcgta aagtgattta cgtaccaggt aaactcctca atctggtcgt
     6541 tggctaagcg cgggaggaag cgtgcgatat ctggcaacat tgttgttatc tctggctgtg
     6601 ttaatcaccg ccgggtgtgg ctggcatctg cgtgatacca cgcaggttcc ttccactatg
     6661 aaggtcatga tcctggactc aggcgatccg aacgggccat taagccgtgc ggtgcgtaac
     6721 cagttacgtc tgaatggtgt cgagttgctt gataaagaaa ccacgcgtaa ggacgttcca
     6781 tccctgcgtt tgggtaaagt gagcatcgcg aaagataccg catcggtatt ccgtaacggt
     6841 caaacagcag agtatcagat gatcatgacg gtgaatgcga ccgtgttgat ccccggccgt
     6901 gatatctacc cgattagtgc caaagtcttc cgttcgttct ttgataaccc gcaaatggcg
     6961 ttagcgaaag ataacgaaca agacatgatc gtaaaagaga tgtacgaccg tgctgccgaa
     7021 cagctgattc gtaagctgcc aagcatccgt gctgcggata ttcgttccga cgaagaacag
     7081 acgtcgacca caacggatac tccggcaacg cctgcacgcg tctccaccac gctgggtaac
     7141 tgatgattcg gttgtacccg gaacaactcc gcgcgcagct caatgaaggg ctgcgcgcgg
     7201 cgtatctttt acttggtaac gatcctctgt tattgcagga aagccaggac gctgttcgtc
     7261 aggtagctgc ggcacaagga ttcgaagaac accacacttt ttccattgat cccaacactg
     7321 actggaatgc gatcttttcg ttatgccagg ctatgagtct gtttgccagt cgacaaacgc
     7381 tattgctgtt gttaccagaa aacggaccga atgcggcgat caatgaacaa cttctcacac
     7441 tcaccggact tctgcatgac gacctgctgt tgatcgtccg cggtaataaa ttaagcaaag
     7501 cgcaagaaaa tgccgcctgg tttactgctc ttgcgaatcg cagcgtgcag gtgacctgtc
     7561 agacaccgga gcaggctcag cttccccgct gggttgctgc gcgcgcaaaa cagctcaact
     7621 tagaactgga tgacgctgca aatcaggtgc tctgctactg ttatgaaggt aacctgctgg
     7681 cgctggctca ggcactggag cgtttatcgc tgctctggcc agacggcaaa ttgacattac
     7741 cgcgcgttga acaggcggtg aatgatgccg cgcatttcac cccttttcat tgggttgatg
     7801 ctttgttgat gggaaaaagt aagcgcgcgt tgcatattct tcagcaactg cgtctggaag
     7861 gcagcgaacc ggttattttg ttgcgcacat tacaacgtga actgttgtta ctggttaacc
     7921 tgaaacgcca gtctgcccat acgccactgc gtgcgttgtt tgataagcat cgggtatggc
     7981 agaaccgccg gggcatgatg ggcgaggcgt taaatcgctt aagtcagacg cagttacgtc
     8041 aggccgtgca actcctgaca cgaacggaac tcaccctcaa acaagattac ggtcagtcag
     8101 tgtgggcaga gctggaaggg ttatctcttc tgttgtgcca taaacccctg gcggacgtat
     8161 ttatcgacgg ttgatatgaa atctttacag gctctgtttg gcggcacctt tgatccggtg
     8221 cactatggtc atcttaaacc cgtggaaacg ctggcgaatt tgattggtct gacgcgggtc
     8281 acaatcatcc ctaataatgt tcctccgcat cgtccccagc cggaagcgaa cagcgtgcag
     8341 cgtaaacaca tgcttgaact ggcgattgcc gacaagccat tatttactct tgatgaacgc
     8401 gagctaaagc gcaatgcccc ctcttacact gcgcaaacac tgaaagagtg gcggcaggaa
     8461 caagggccgg acgtgccgct ggcgtttatt attggtcagg attcattgct gacctttccg
     8521 acctggtacg aatacgaaac gatactcgac aatgcacatt tgatcgtctg tcggcgtcca
     8581 ggttacccac ttgaaatggc gcaaccgcaa taccagcaat ggctggaaga tcatttgaca
     8641 cataacccgg aagatcttca ccttcagcct gccggtaaaa tttatctggc tgaaacgccg
     8701 tggtttaaca tctcggcgac catcatccgc gaacgtttgc aaaacggtga atcgtgtgag
     8761 gatttattgc cggaaccggt actgacttac attaaccaac aaggcttgta tcgctgatac
     8821 ctgtcgttac ggagggcatg atgcgactgt ggttaattcg tcatggtgaa acgcaagcga
     8881 atatcgatgg tctttacagc ggtcatgcgc ccacccccct gaccgcgcgc ggtattgagc
     8941 aagcgcaaaa tctgcataca ctgctagatg atgtttcctt tgacctggtt ttatgcagtg
     9001 aactggaacg ggcacagcat accgcgcgac gggttctcag tgaccgccag ctccccgtgc
     9061 aaatcatccc tgaactcaac gaaatgtttt ttggcgactg ggagatgcga catcatcgcg
     9121 acctcatgca agaagatgcc gaaaactata gcgcgtggtg caatgactgg cagcatgcta
     9181 tccccacgaa cggtgaagga tttcaggcat tttcgcaacg tgtggaaaga tttatcgcaa
     9241 ggcttagtga atatcagcac tatcagaata ttttaatcgt cagccatcag ggcgtactga
     9301 gtctgttaat tgctcgtcta attggcatgc ctgccgaatc tatgtggcat tttcgcgttg
     9361 accaggggtg ttggagcgcc attgatatca accaaaaatt cgcgacgcta cgcgtcctca
     9421 atagccgtgc catcggggtc gagaatgcat gacttttctg tttttttacg ggtaagccgc
     9481 aacgaccatt gacagccccg ggcaggctga tattctccgc agccagactt tttccgccag
     9541 acacgacttt gtagaaattg ttttacaaaa atggcgatgc aatctgcggc gcggggtggg
     9601 atgatagccc actttcgaaa gccgattcgg cgacaattgt cccgaaatcg cctctggttc
     9661 aggtatactg acagaccatt tttatctatt tgattcaccc agggggaaaa cttgcagggt
     9721 aaagcactcc aggattttgt tatcgacaaa attgatgacc tcaaaggtca ggacatcatc
     9781 gccttagacg ttcagggcaa atccagcatc accgactgca tgatcatctg tacgggtacg
     9841 tccagccgtc atgttatgtc cattgctgac cacgttgtgc aggagtctcg cgcagcgggc
     9901 ctgttaccgc tcggcgtaga aggtgaaaac agcgccgact ggattgtcgt ggatttgggc
     9961 gatgtgattg tccatgtcat gcaggaagag agccgtcgcc tgtatgaact ggaaaaactc
    10021 tggagttaat gcgtgaagct gcaacttgtc gccgtgggaa cgaaaatgcc ggactgggta
    10081 caaaccggtt ttaccgagta cctgcgtcgt tttccgaaag atatgccctt cgagctgatt
    10141 gaaattccgg ccggaaaacg cggcaagaat gcggacatca agcgcatact cgacaaagag
    10201 ggtgagcaga tgttggcggc cgcaggcaaa aaccgcattg tcaccctcga tattccaggc
    10261 aagccctggg atacgccgca gttagccgct gagctggaac gctggaagct ggatggtcgc
    10321 gacgtcagtc tactgattgg cgggcctgaa gggttgtcgc ctgcctgtaa agcggcggcg
    10381 gagcagagct ggtcgctgtc ggcgcttacc ctcccccatc cgctggttcg cgtgctggtc
    10441 gcagagagtc tgtaccgggc gtggagcatc accaccaacc atccttatca ccgtgagtga
    10501 taaaggagct ttgagtagaa aacgcagcgg atgaaactac agaactcttt tcgcgactat
    10561 acggcagagt ccgcgctgtt tgtgcgccgg gcgctggtcg cctttttggg gattttgctg
    10621 ctgaccggcg tgcttatcgc caacctgtat aatctgcaaa ttgttcgctt taccgattac
    10681 cagacccgct ctaatgaaaa ccgcattaag ctggtgccta tcgcgcccag ccgcggcatt
    10741 atctacgacc gtaacggtat ccctctggca ctcaaccgca ctatctacca gatagaaatg
    10801 atgccggaga aagtcgataa cgtgcagcaa acgctggacg ctttgcgcag cgtggttgat
    10861 ctgaccgatg acgatattgc tgcattccga aaagagcgcg cacgttcaca ccgtttcacc
    10921 tctattcccg tgaaaactaa cctgaccgaa gtacaagtag ctcgctttgc cgtcaatcag
    10981 taccgttttc cgggtgtcga agttaaaggc tataaacgtc gttactatcc ttacggttcg
    11041 gcgttgaccc acgtcatcgg ctatgtgtcg aaaatcaacg ataaagacgt cgaacgcctg
    11101 aataatgacg gcaaactggc caactatgcg gcaacgcatg atatcggtaa gctgggcatt
    11161 gagcgttact atgaagacgt gctgcacggt cagaccggtt atgaagaggt tgaagttaac
    11221 aaccgtgggc gtgttatccg ccagttaaaa gaagtaccac cgcaagccgg acacgatatt
    11281 tacctgacgc tggatctcaa actccagcaa tatattgaaa cgctgctggc gggtagccgc
    11341 gcagctgtgg tagtcaccga tccgcgtaca ggtggggtgc tggcactggt ttccacgcct
    11401 agttatgacc caaacctgtt tgttgacggt atctccagca aagattattc cgccttgctg
    11461 aacgacccga atacaccgct ggtgaaccgc gccacacagg gggtttatcc tcctgcgtct
    11521 acagttaaac cctatgtggc ggtttcggca ttaagcgccg gggtgatcac gcgcaatacg
    11581 acgctgtttg acccaggctg gtggcaacta ccagggtcgg aaaaacgtta tcgtgactgg
    11641 aaaaaatggg gccacgggcg tctgaatgtc acaagatcgc tggaagaatc tgcggatacc
    11701 ttcttctatc aggtggccta cgatatgggg atcgatcgcc tctccgaatg gatgggtaaa
    11761 ttcggttatg gtcattacac cggtatcgac ctggcggaag aacgttccgg caacatgcct
    11821 acccgcgaat ggaaacagaa acgctttaaa aaaccgtggt atcagggtga caccattccg
    11881 gttggtatcg gccagggtta ctggacagcg accccaatcc agatgagtaa ggcactgatg
    11941 atcctgatta atgacggtat cgtgaaggtt ccgcatttgc tgatgagcac cgccgaagac
    12001 ggcaaacagg tgccatgggt acaaccgcat gaaccgcccg tcggcgatat tcattccggt
    12061 tactgggagc tggcgaaaga cggtatgtac ggtgttgcta accgccctaa cggtacggcg
    12121 cataaatact ttgccagcgc accgtacaaa attgcggcga aatctggtac cgctcaggtc
    12181 ttcggtctga aagcgaacga aacctataat gcgcacaaaa ttgccgagcg tttacgtgac
    12241 cacaaactga tgaccgcctt tgcgccatac aacaatccgc aagtggctgt cgccatgatt
    12301 ctggagaacg gcggcgcggg tccggcggtt ggtacgctga tgcgccagat cctcgaccac
    12361 attatgctgg gtgataacaa taccgatctg cctgcggaaa atcctgcggt tgccgcagcg
    12421 gaggaccatt aatcatgacg gataatccga ataaaaaaac attctgggat aaagtccatc
    12481 tcgatcccac aatgctgctg atcttactgg cattgctggt ttacagcgcc ctggttatct
    12541 ggagcgccag cggtcaggat attggcatga tggagcgtaa aataggccaa atcgcgatgg
    12601 gtctggtcat catggtggtg atggcgcaaa ttcctccacg cgtttatgaa ggctgggccc
    12661 cctatctcta tatcatctgt attattttgc tggtggcggt agatgctttc ggtgccatct
    12721 ctaaaggcgc tcaacgctgg ctggacctcg gtattgttcg ttttcagccg tcggaaattg
    12781 ccaaaatagc cgtaccactg atggttgcgc gctttatcaa ccgcgacgtt tgcccgccat
    12841 cgttgaagaa cactggcatc gcgctggtgc tgatatttat gcccacgctg ctggtggctg
    12901 cacagcctga cctgggaaca tcaatcctcg ttgcgctttc cggtctgttt gtactgttcc
    12961 tctctggcct tagctggcgt ctgattggcg tcgcagtagt gctggtggcg gcgttcattc
    13021 ctattctgtg gttcttcctg atgcatgatt accagcgcca gcgcgtaatg atgctcctgg
    13081 acccggaatc agacccactc ggcgcgggct atcacattat tcagtctaaa attgctattg
    13141 gctccggcgg attacgcggc aaaggctggc tgcatggcac tcagtcacaa cttgaatttc
    13201 tacccgaacg ccataccgac tttatcttcg cggtactggc ggaagagctg ggattagtgg
    13261 gcattctgat tctgctcgct ctctacattc tgctgatcat gcgcgggctg tggatagccg
    13321 ccagagcgca aaccaccttt ggtcgcgtca tggctggcgg cttaatgctg atattattcg
    13381 tttatgtctt cgtaaatatt ggtatggtaa gcggtattct gccggttgta ggggttccgc
    13441 tcccactggt cagttatgga ggatcagcgc taattgtgct gatggctggg ttcgggattg
    13501 taatgtcaat ccacacccac aggaaaatgt tgtcgaaaag cgtgtaagag gtgcgcaatg
    13561 cgtaagcagt ggctcgggat ctgcatcgcg gcaggaatgc tcgcggcatg tacaagcgat
    13621 gatggtcagc aacagacggt aagtgtaccg cagcctgcgg tatgtaacgg ccccatagtt
    13681 gaaattagcg gggcggaccc gcgtttcgaa ccactgaacg cgacggcaaa tcaggattac
    13741 cagcgcgacg gtaaaagcta caaaatcgtg caggatccgt ctcgatttag ccaggcggga
    13801 ctggcggcaa tctatgatgc cgaaccaggc agtaacctga cggcctctgg cgaagctttc
    13861 gatccgacac agttgacggc ggcccatcca acgcttccga tccccagcta cgccagaatc
    13921 actaacctgg ctaacgggcg aatgatcgtg gtgcgcatta atgatcgcgg cccctacggc
    13981 aacgaccgcg taatttcgct ttctcgcgca gcagctgacc gtcttaacac gtcaaacaac
    14041 accaaagttc gtatcgatcc gattattgtc gcccaggatg gttcgctttc tggtcctggt
    14101 atggcttgta ccacagtcgc caaacagact tacgccctgc ctgcacctcc cgatttaagc
    14161 ggtggcgcgg gggcaagttc agtgtctggc ccgcagggtg acattcttcc ggtcagtaac
    14221 tcgacgctaa aaagcgaaga tccgaccggc gcgccggtaa ccagtagcgg tttcctcggc
    14281 gctccaacga ccttagcgcc tggtgtactg gaaagcagcg aaccgacacc tgcaccacag
    14341 cccgttgtta cagctccgtc gacaacgcct gcaacctcgc ctgcaatggt gacaccgcaa
    14401 gccgcctcgc aaagcgccag cggcaacttt atggtgcaag tcggggccgt tagcgatcag
    14461 gctcgtgcgc aacagtacca acagcaactg ggacagaagt tcggcgtccc cggtcgcgta
    14521 actcaaaatg gcgcggtctg gcgtatccag cttggcccat tcgccagtaa agccgaagcc
    14581 agtaccttgc agcaacgttt gcaaaccgaa gcccaattac agtcatttat taccaccgcg
    14641 cagtagcgta aagcaggcat ctgatgtgtc aatttctgta aatgacgtta acaaagtcac
    14701 gcggaaagtc agatgcctgc cggtagtgca tttgctatag tagggcactt tttttaattc
    14761 catcacggat gtcgtagttc agaccatgaa taccattttt tccgctcgta tcatgaagcg
    14821 cctggcgctc accacggctc tttgcacagc ctttatctct gctgcacatg ccgatgacct
    14881 gaatatcaaa actatgatcc cgggtgtacc gcagatcgat gcggagtcct acatcctgat
    14941 tgactataac tccggcaaag tgctcgccga acagaacgca gatgtccgcc gcgatcctgc
    15001 tagcctgacc aaaatgatga ccagttacgt tatcggccag gcaatgaaag ccggtaaatt
    15061 taaagaaact gatttagtca ctatcggcaa cgacgcatgg gccaccggta acccggtgtt
    15121 taaaggttct tcgctgatgt tcctcaaacc gggcatgcag gttccggttt ctcagctgat
    15181 ccgcggtatt aacctgcaat cgggtaacga tgcttgcgtc gccatggccg attttgccgc
    15241 tggtagccag gacgcttttg ttggcttgat gaacagctac gttaacgccc tgggcctgaa
    15301 aaacacccac ttccagacgg tacatggtct ggatgctgat ggtcagtaca gctccgcgcg
    15361 agatatggcg ctgatcggcc aggcattgat ccgtgacgta ccgaatgaat actcgatcta
    15421 taaagaaaaa gaatttacgt ttaacggtat tcgccagctg aaccgtaacg gcctgttatg
    15481 ggataacagc ctgaatgtcg acggcatcaa aaccggacac actgacaaag caggttacaa
    15541 ccttgttgct tctgcgactg aaggccagat gcgcttgatt tctgcggtga tgggcggacg
    15601 tacttttaaa ggccgtgaag ccgaaagtaa aaaactgcta acctggggct tccgtttctt
    15661 cgaaaccgtt aacccactga aagtaggtaa agagttcgcc tctgaaccgg tttggtttgg
    15721 tgattctgat cgcgcttcgt taggggttga taaagacgtg tacctgacca ttccacgtgg
    15781 tcgcatgaaa gatctgaaag ccagctatgt gctgaacagc agtgaattgc atgcgccgct
    15841 gcaaaagaat caggtcgtcg gaactatcaa cttccagctt gatggcaaaa cgatcgagca
    15901 acgcccgctg gttgtgttgc aagaaatccc ggaaggtaac ttcttcggca aaatcattga
    15961 ttacattaaa ttaatgttcc atcactggtt tggttaaaaa ttgaacactt gaaagtgtaa
    16021 tttccgtccc catatactaa gcatcagtaa aaaaactccc gccttctggc gggagttgct
    16081 atttaattac gttacgccgg agctgacatg aaaaccaaac ttaacgaact gcttgaattc
    16141 cctactcctt ttacttacaa agttatgggg caggcgttac ctgagctggt tgatcaggtg
    16201 gttgaagtgg tacagcgcca tgcgccaggt gactacaccc caacggtaaa accaagcagc
    16261 aaaggcaact accactcggt atctatcact atcaacgcca ctcatatcga gcaggttgaa
    16321 acactgtatg aagaactggg caaaatcgat attgtccgca tggttctgta actcgcttct
    16381 ccgttacccg gctcctggtc gggtaactcc cctctcctcg ctgtgatata cttttccccc
    16441 acttttactc attctccacg gagatgccgt tttgtatcag gataaaattc ttgtccgcca
    16501 gctcggtctt cagccttacg agccaatctc ccaggctatg catgaattca ccgatacccg
    16561 cgatgatagt acccttgatg aaatctggct ggtcgagcac tatccggtat tcacccaagg
    16621 tcaggcagga aaagcggagc acattttaat gccgggtgat attccggtga tccagagcga
    16681 tcgcggtggg caggtgactt atcacgggcc gggacaacag gtgatgtatg tgttgcttaa
    16741 cctgaaacgc cgtaaactcg gtgtgcgtga actggtgacc ttgcttgagc aaacagtggt
    16801 gaataccctg gctgaactgg gtatagaagc gcatcctcgg gctgacgcgc ctggtgtcta
    16861 tgtcggggaa aagaaaattt gctcactggg tttacgaatt cgacgcggtt gttcattcca
    16921 cggtctggca ttaaacgtca atatggatct ttcaccattt ttacgtatta atccttgtgg
    16981 gtatgccgga atggaaatgg ctaaaatatc acaatggaaa cccgaagcga cgactaataa
    17041 tattgctcca cgtttactgg aaaatatttt agcgctacta aacaatccgg acttcgaata
    17101 tattaccgct taattccata catcaatggc ccaatttaca ctgggtcatt actcataata
    17161 cctgctaatg ctttacatct ccttcgcgtt tgtatatttt ctttatcact tgcagaaatg
    17221 cggtgccacg ccttgctccg caactcaccc cattgattaa cctattgaaa tagttgatta
    17281 tggtgagcaa aag
//
LOCUS       DAZGLJ010000097        17292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000097 DAZGLJ010000000
VERSION     DAZGLJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.097"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            92..676
                     /locus_tag="ACHK49_002716"
     CDS             92..676
                     /locus_tag="ACHK49_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9263337.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            756..1451
                     /locus_tag="ACHK49_002717"
     CDS             756..1451
                     /locus_tag="ACHK49_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9263338.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1481..3997
                     /locus_tag="ACHK49_002718"
     CDS             1481..3997
                     /locus_tag="ACHK49_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO9263339.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNRHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLKQNLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            4008..5099
                     /locus_tag="ACHK49_002719"
     CDS             4008..5099
                     /locus_tag="ACHK49_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9263340.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5142..6002)
                     /gene="rsmI"
                     /locus_tag="ACHK49_002720"
     CDS             complement(5142..6002)
                     /gene="rsmI"
                     /locus_tag="ACHK49_002720"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO9263341.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            6066..8102
                     /gene="lpoA"
                     /locus_tag="ACHK49_002721"
     CDS             6066..8102
                     /gene="lpoA"
                     /locus_tag="ACHK49_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO9263342.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            8060..8455
                     /locus_tag="ACHK49_002722"
     CDS             8060..8455
                     /locus_tag="ACHK49_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO9263343.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8475..9065
                     /gene="diaA"
                     /locus_tag="ACHK49_002723"
     CDS             8475..9065
                     /gene="diaA"
                     /locus_tag="ACHK49_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO9263344.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            9075..9650
                     /gene="dolP"
                     /locus_tag="ACHK49_002724"
     CDS             9075..9650
                     /gene="dolP"
                     /locus_tag="ACHK49_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO9263345.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9764..10804)
                     /locus_tag="ACHK49_002725"
     CDS             complement(9764..10804)
                     /locus_tag="ACHK49_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO9263346.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMLYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10877..11512)
                     /locus_tag="ACHK49_002726"
     CDS             complement(10877..11512)
                     /locus_tag="ACHK49_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO9263347.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            11640..12158
                     /gene="yhbO"
                     /locus_tag="ACHK49_002727"
     CDS             11640..12158
                     /gene="yhbO"
                     /locus_tag="ACHK49_002727"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO9263348.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12138..12581)
                     /locus_tag="ACHK49_002728"
     CDS             complement(12138..12581)
                     /locus_tag="ACHK49_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO9263349.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12632..12934
                     /gene="yhbQ"
                     /locus_tag="ACHK49_002729"
     CDS             12632..12934
                     /gene="yhbQ"
                     /locus_tag="ACHK49_002729"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO9263350.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12921..13424)
                     /locus_tag="ACHK49_002730"
     CDS             complement(12921..13424)
                     /locus_tag="ACHK49_002730"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9263351.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13418..13942)
                     /locus_tag="ACHK49_002731"
     CDS             complement(13418..13942)
                     /locus_tag="ACHK49_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO9263352.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14151..15146
                     /locus_tag="ACHK49_002732"
     CDS             14151..15146
                     /locus_tag="ACHK49_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO9263353.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15155..16033
                     /locus_tag="ACHK49_002733"
     CDS             15155..16033
                     /locus_tag="ACHK49_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO9263354.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16239..17246
                     /locus_tag="ACHK49_002734"
     CDS             16239..17246
                     /locus_tag="ACHK49_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO9263355.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
BASE COUNT         4369 a         4570 c         4363 g         3990 t
ORIGIN      
        1 gaaatatttc tacctgtata atctttaaag atgttgaaca tatattcaca ttaaatatga
       61 ttatgtactt gttacaagga taaggttata aatgaataaa gttacaaaaa cagctattgt
      121 gggcttactt gcgcttttcg caggtaatgc cgctgcaacc gatggtgaaa tagtttttga
      181 tggcgagatt ttaaaatccg cttgtgaaat caatgactct gataagaaaa ttgaagttgc
      241 tcttggtcac tataatgccg aacagtttcg ttctgttggc gatcgtagcc caaaaattcc
      301 attcactatt cctttagtta actgcccagt gactgggtgg gagcacgata acggcaacgt
      361 tgaagcgtct ttccgtttat ggctggaaac gcgggataat ggcaccgtcc ctaacttccc
      421 taatctggca aaagtgggct cttttgctgg cacagcggca acaggtgtgg gtatccgtat
      481 tgacgatgcg gaaagcggaa accttatgcc actgaatgct atggggaatg ataacacggt
      541 ttatcagatc ccagcggact ccaatggtat tgtcaatgtt gacctcatcg cttactacgt
      601 atcaaccgtt gaagcatcag aaatcacccc aggggaagca gacgctgtcg ttaacgtaac
      661 gctggattac cgttaatacg ttacggcgtt atctgacctt tcagataacg cccttttcct
      721 tcctctttct cgttgtatca ggttgaaaaa taactatgtc aaaacgaaca ttcgcggtga
      781 taataacctt gttgtgtagc ttctgtatcg gccaggcgct tgcagggggt atcgttttac
      841 agcgaacgcg agtgatctat gatgccagcc gcaaagaggc tgcgttacct gtcgcaaaca
      901 aaggcgcaga aacgccttat ttactgcaat catgggtaga taatatagat ggtacaagcc
      961 gtgccccatt tattataacc ccaccgctat ttcgtcttga agccggcgat gactcatcac
     1021 tgcgaattat taaaacagct gataacctgc ctgaaaataa agagtcgctg ttctacatta
     1081 atgttcgtgc cattccagca aagaaaaaat cagatgatgt taatgctaac gagttgacgc
     1141 tggtatttaa aacacggatc aaaatgtttt atcgccccgc acacctgaag ggacgggtaa
     1201 acgatgcgtg gacatcactg gaatttaaac gtagtgacca ttcactcaat atatataacc
     1261 caactgaata ttacgtcgta tttgccggac tggcagtcga taaaaccgat ctcacaagca
     1321 aaattgaata tatcgcgccc ggagaacata aacagttacc acttcctgca tctggcggaa
     1381 agaacgtgaa atgggctgca atcaatgatt atggcggcag ttccgggaca gaaactcgtc
     1441 cactgcaata acaaatataa aaaacacagg tcatcaggga atgccacaac gacaccacca
     1501 gggacataaa cgcacaccga aacagttggc gctcatcatc aaacgctgtt tgccgatggt
     1561 gctcactggc agcggcatgc tttgcactac cgctaacgcc gaagagtatt atttcgaccc
     1621 cattatgctg gaaactacaa aaagtggtat gcaaacaact gatctgtcac gtttttcaaa
     1681 aaaatacgca caactaccag gaacttatca ggttgatatc tggctgaata aaaagaaggt
     1741 ttcacagaaa aaaattacat ttaccgccaa tgcagagcaa cttctgcagc cacagtttac
     1801 ggtagaacaa ctacgtgagc tgggtattaa ggtggatgaa atcccggcgc tggctgaaaa
     1861 agatgacgat agcgtgatca actcgcttga acaaatcatt cccggtacag ctgctgaatt
     1921 tgatttcaat catcagcgac ttaatttgag cattccccaa attgcactgt accgtgatgc
     1981 aagaggttac gtctcccctt ctcgttggga cgatggtata ccaacgctgt ttaccaacta
     2041 ctcgtttaca ggttctgata accgttaccg ccagggcaat cgtagccaac gacagtacct
     2101 gaatatgcaa aatggtgcta attttggccc ctggcgatta cgtaactatt ctacgtggac
     2161 acgcaacgat caggcgtcaa gctggaacac tatcagtagt tatttacaac gtgatatcaa
     2221 ggcgttgaag tctcagttgc ttctgggaga aagcgccacc agcggcagta ttttttccag
     2281 ctacaccttt actggcgtgc aactcgcttc cgacgataat atgttgccaa acagccagcg
     2341 cggatttgcc ccaacggtac gcggtatcgc aaacagtagt gcaatcgtga ctatcaggca
     2401 aaatggttat gtgatctatc aaagcaacgt gccagcgggt gcctttgaaa ttaacgatct
     2461 ctacccctct tccaacagcg gcgatttaga agtcacgatt gaagaaagtg acggtacgca
     2521 acgtcgcttt atccagcctt attcttcatt acccatgatg cagcgacctg ggcatctaaa
     2581 atatagcgcg accgctggac gctatcgcgc tgatgcaaac agtgatagca aggaacccga
     2641 atttgctgaa gccacggcaa tatatggttt gaataatact tttacgctgt atggcggcct
     2701 gctcggttct gaagattatt atgcgctggg gatcggtatc ggcggcacac ttggcgcact
     2761 gggcgcgttg tcgatggata tcaacagagc tgacacccaa ttcgataacc ggcactcttt
     2821 tcatggctat caatggcgta cgcagtacat caaagatatc ccggaaacca acaccaatat
     2881 cgctgttagc tactatcgct ataccaacga tggctatttt agttttgatg aagccaatac
     2941 ccgtaattgg gactataaca gtcgccaaaa aagtgaaatt caattcaaca tcagccagac
     3001 aatatttgat ggggtaagtc tgtatgcctc cggttcgcag caagactatt ggggcaataa
     3061 cgataaaaac aggaatatct cagttggggt ttccggtcag caatggggaa ttggttacag
     3121 cctgaattat caatacagtc gctacacgga tcaaaataat gaccgcgcac tctctttgaa
     3181 tctcagtatt ccgttagaac gctggttacc gcgtagccgg gtttcctatc agatgaccag
     3241 ccagaaagat cgcccaaccc aacatgaaat gcgtcttgat ggctcactgc tggatgatgg
     3301 tcgcctgagc tatagcctga aacaaaatct ggatgacgat aacaaccata acagtagcct
     3361 gaacgccagt taccgttcac cttatggcac cttcagtgcc ggatacagct acggtaatga
     3421 cagcagccaa tacaattacg gcgttaccgg cggcgtggtt atccatcctc atggcgtgac
     3481 gctctcgcaa tatctgggca acgcttttgc gcttatcgat gctaatgggg cttctggcgt
     3541 gaggatacaa aactatccgg ggattgctac tgatcccttt ggctatgcag tggttcctta
     3601 tctcacgact taccaggaaa accgtctctc ggtagatact acgcagctgc ccgataacgt
     3661 cgatcttgag caaacaacac agtttgtggt gcccaacaga ggtgcaatgg tagcggcgcg
     3721 tttcaacgcc aatatcggtt atcgcgtact tgttacagtc agcgatcgca acggtaaacc
     3781 gttgcccttt ggcgctcttg ccagcaacga tgagacgggg caacaaagta tcgtcgatga
     3841 gggcggcata ctatatctct ctgggatatc gagtaaatca caaagctgga ctgtacgctg
     3901 gggaaatcag gcagatcaac aatgtcagtt tgcttttagt acaccggatt cagaaccaac
     3961 aacctctgta ttacaaggca cggcgcagtg ccattaagga taaaaaaatg aaaagagcgc
     4021 ctcttataac aggacttttg ttgatatcca catcctgcgc ttatgcctcc tcaggagggt
     4081 gtggagctga cagcactagc ggtgcgacaa attacagcag tgtggttgat gatgttacgg
     4141 tgaaccagac agataacgtg acaggacggg agtttacctc tgccacgcta agtagcacta
     4201 actggcaata cgcctgtacc tgctctgcgg gtaaggcagt taaacttgtc tatatggtca
     4261 gccctgtact taccaccact ggacatcaag caggatatta caaactcaat gatagtctgg
     4321 atattaaaac cacattacag gcgaatgaca ttccaggact cgtgactgac cagaccgttt
     4381 ctgttaacac ccgattcaca cagataaaaa gcaacaccgt atattctgct gcaacccaaa
     4441 cgggtgtttg ccagggtgac acgtctcgtt atggacccgt taatattggt gcaaatacca
     4501 cctttaccct gtatgtcacc aagccatttc tcggctcgat gaccattccg aaaacggata
     4561 ttgccgtcat taaaggcgca tgggtcgatg gaatgggaag cccgtctaca ggtgacttcc
     4621 atgatttagt caagttatcg attcagggaa atctcaccgc cccacagtcg tgcaaaatta
     4681 atcagggcga tgttattaag gttaattttg gattcatcaa tggtcagaag tttaccaccc
     4741 gcaatgccat gccagacggt tttactccag tagactttga tatcacttat gactgtggtg
     4801 atacttcaaa gattaaaaac tcgttgcaaa tgcgcatcga cggtacaact ggggtagtag
     4861 accagtacaa cctggtcgcc agacgaagaa gttcagacaa tgcgcccgat gtcggtattc
     4921 gtattgaaaa tctcggcggc ggagttgcaa atattccttt tcagaacggt atccttcccg
     4981 ttgatccttc cgggcatggc accatcaaca tgcgcgcctg gccagttaat ctggtcggtg
     5041 gtgagctgga aacaggaaaa tttcagggca ccgccaccat taccgtcatc gtgcggtaaa
     5101 agaagacgtt gcgttttgat taacgcaacg cttcggcact cttacccctg ctgctccagc
     5161 gcatacttat acagcgcatt tttcttcacg ccgtgaattt ctgccgccag cgccgccgct
     5221 tttttcaacg gcagttctgc ctgtagcagc gccagcgtgc gcagggcatc ggcgggtaag
     5281 tcttcttcct gtgctttatg accttcgata atcagcacca tttcgccttt gcgacggttt
     5341 tcatcttctt ttacccacgc cagcagctcg ccaacgggcg cgccgtgaat ggtttcccag
     5401 gttttggtca gctcacgcgc cagaaccacg tagcgggatt cgcctaatac cgcaacgata
     5461 tcttccaggc tatctaacag acggtgggtg gattcataaa aaatcagcgt gcgcggctcc
     5521 gcttcaatgg cttttagcgc atcacggcgg ccttttgatt tggcaggtaa aaagccttcg
     5581 taacagaaac ggtcagaggg taaacccgcg gcgcttaacg cagtgatagc agcacacggc
     5641 ccgggtagcg gcaccacgca aatccccgct tcacggcagg tacgcaccag atggtagcca
     5701 ggatcgttaa ttagcggcgt tccggcatcg gaaaccagcg caatgttctg cccttcttgc
     5761 agcttcgcca gcagcgtttc ggctttttgt tgttcgttat ggtcgtgcag cgcaaacaac
     5821 cgggcattaa tcccaaaatg ttgcagcaat aaaccggtgt gacgagtatc ctcggcggca
     5881 atcagatcaa cggcctgtaa tacctctaac gcacgctggg tgatatccgc cagattgccg
     5941 attggcgtcg gtacaatgta aagctggccc tgagaattat ccgccgattg gtgttgtttc
     6001 attgtgtcgt ccgtattgcc gatttaatat tgagcattgc gtaaaaaaat atcactggat
     6061 acattatggt accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg cctgttgttc
     6121 tggcagccct gattttcgcc ggttgtggca cccatactcc cgatcagtcc actgcttata
     6181 tgcagggcac ggcgcaggct gattctgcct tttatcttca gcagatgcag caaagctctg
     6241 atgataccag gatcaactgg caattactcg ccattcgtgc actggtgaaa gaaggtaaaa
     6301 ccgggcaggc ggttgagttg tttaaccaac taccgcaaga actgaacgat tctcagcgtc
     6361 gcgagaaaac actgctggcg gtagagatta aactggcgca gaaagatttt gctggcgcgc
     6421 aaaacttgct ggcgaaaatc acacctgccg atttagaaca aaaccagcaa gcgcgttact
     6481 ggcaggcaaa aatcgatgcc agccaggggc gtccttccat tgatttactg cgcgcgttaa
     6541 ttgctcagga accgctgctc ggcgcgaaag aaaaacagca gaatattgat gccacctggc
     6601 aggcgctctc ctccatgact caggaacagg cgaatacgct ggtgatcaac gccgacgaaa
     6661 atattctgca aggctggctg gatctgcagc gcgtctggtt tgataaccgt aacgatcccg
     6721 acatgatgaa agccgggatc gccgactggc aaaaacgtta tccgaacaat ccgggcgcga
     6781 aaatgctgcc aacgcagttg gttaacgtaa aagcgtttaa accagcctcg accaacaaaa
     6841 tcgccctgct gttgccgctg aatggccagg cagcggtatt tggtcgcact attcagcaag
     6901 gctttgaagc ggcgaaaaat atcggcactc agccagtggc ggctcaggta gctgccgcac
     6961 ctgccgcaga cgtagctgaa caacctcagc cgcaaaccgt ggatggcgtt gccagcccgg
     7021 cacaagcctc ggttagcgat ctgaccggtg aacagcctgc ggcacagcca gcgcctgtaa
     7081 gcaccccggc agcaaccacc acagcggtaa gcgcgccagc gaatccatcc gcagaactga
     7141 aaatctacga tacctcatca caaccactta gccagatctt aagccaggtt cagcaggatg
     7201 gcgcgagtat tgtggttggt ccgttgctga aaaataacgt tgaagagttg cttaagagca
     7261 acaccccgct gaacgtgctg gcactgaacc agccggagaa tatcgaaaac cgcgtcaata
     7321 tttgttactt cgcgctttca ccggaagacg aagcgcgcga tgcagcgcgt catattcgtg
     7381 accagggtaa acaagcgccg ctggtgctga tcccacgcag ttcattgggc gatcgcgtag
     7441 ccaatgcgtt tgcgcaggag tggcagaaac tgggcggcgg caccgttctg caacaaaaat
     7501 ttggttccac cagcgaatta cgcgcgggtg ttaacggcgg ttctggtatc gctttaacgg
     7561 gtaccccgat tactcccaga gcgacaaccg actccggcat gacgaccaac aatccaacgc
     7621 tgcaaaccac gccaaccgat gaccagttca ccaataatgg cggtcgtgtc gatgcggtgt
     7681 acattgtggc aacgccgggt gaaatcgctt ttatcaaacc gatgatcgcc atgcgtaacg
     7741 gtagccagag cggtgcaacg ctgtacgcca gctcccgcag tgcgcaaggg accgctggcc
     7801 cggatttccg actggagatg gaaggcttgc agtacagcga aatcccgatg ctggcaggcg
     7861 gtaatctacc gttaatgcag caggcactca gcgcggtgaa taacgattat tcactggctc
     7921 gcatgtatgc gatgggcgtc gatgcctggt cgctggcaaa tcatttctca caaatgcgcc
     7981 aggttcaggg ttttgaaatc aacggtaata ccggaagcct gacggcaaac ccggattgcg
     8041 tgattaacag gaagttatca tggctacagt accaacaagg tcaggtagtc cccgccagtt
     8101 aaccaccaaa cagaccggcg atgcgtggga agtacaagcg cgtcgctggc tggaaggcaa
     8161 aggactgcga tttgtcgccg ctaacgtgaa cgagcgtggc ggcgagatcg atctgataat
     8221 gcgtgaaggc cggaccaccg tttttgtcga ggtacgctat cgccgctctg cgctttatgg
     8281 cggcgcggca gccagtgtga cccgcagcaa acaacacaaa ttattacaga ctgcccgctt
     8341 gtggctcgcg cgtcataatg ggagttttga tactgtggat tgccggttcg atgtggtagc
     8401 cttcaccggg aatgaggttg agtggattaa ggatgccttt aatgaccact cataattaag
     8461 gtttaaggat tagcgtgcaa gaaagaatta aagcttgctt cactgaaagc attcaaactc
     8521 aaattgcggc ggcagaggcg cttccggatg ccatctcccg tgcagccatg acgctggttc
     8581 agtctctgct caatggcaac aaaatcctct gttgtggtaa tggaacttcc gctgccaatg
     8641 cacagcattt tgctgccagc atgatcaacc gtttcgaaac ggagcggccc agcttacctg
     8701 ccattgcact aaatactgat aatgttgtct taacggcgat tgccaacgat cgcttacatg
     8761 atgaagtgta tgcaaaacag gtgcgggcgc tgggtcatgc gggagatgta ttgttagcca
     8821 tttccacccg tggcaacagc cgcgatattg taaaagcggt agaagctgcc gttacgcgtg
     8881 atatgactat tgtggcattg accggctatg acggcggcga acttgcaggt ttgttagggc
     8941 cacaagatgt ggagatccgc attccttcgc atcgtagtgc tcgcattcag gaaatgcata
     9001 tgctgacggt aaattgcctg tgcgatctga tcgataacac gcttttccct caccaggatg
     9061 tttaaggaga atacatgaag gcattatcgc caatcgcagt ccttatttcc gcgctgctgt
     9121 tgcaaggttg tgttgccgct gccgtagtgg gtaccgctgc tgtgggtacc aaagccgcaa
     9181 ctgacccacg cagtgtcggc acccaggtgg acgatggtac cctggaagtg cgcgtgaaca
     9241 gcgcattgtc gaaagacgaa cagattaaga aagaagcccg tattaatgtc acggcgtatc
     9301 agggcaaagt gctgctggtt gggcagtcac caaacgctga actttcggct cgcgccaaac
     9361 agattgctat gggcgtagac ggtgccaacg aagtgtataa cgagattcgt cagggccagc
     9421 cgattggtct gggcgaagcg tctaacgata cgtggatcac caccaaagtg cgttcgcagc
     9481 tcttaaccag cgacctggtg aaatcgtcca acgtgaaagt gaccaccgaa aacggtgaag
     9541 tgttcctgat ggggctggtg actgaacgtg aagcgaaagc ggcggcagat attgccagcc
     9601 gggtgagcgg cgtaaagcgg gtaaccacag cgtttacgtt tattaaatag cagccccttg
     9661 taatgcctga tgcgacgctt gccgcgtctt atcaggccta caacgatgca aaccgtaggt
     9721 cggataaggc gtttacgccg catccgacat gttttccctc aaatcagaac aacagcgcca
     9781 gcccgccgac aatcacacca gacactgcca ccatcgcccc tgtcagccat aaggctttcg
     9841 ctgggaacgc tttgcgcagc ataatcagtg acggcaaact caccgccggg agcgtcatca
     9901 acaatgccag cgccggagca gttcccatac ctgccagcat catcgtttgt acaatcggaa
     9961 tttctgctgc cgtgggaatg acaaacaagc atcctgctac cgccatcgcc atcacccaca
    10021 tcaggctgtt atcgacagca ccatcggcat gggggaataa ccagacgcga gcggcaccca
    10081 acaccagtac tgcaaggatg taaaccggga tcgtactcca gaaaagcgtc catagcgccc
    10141 tgccccagcg gctaaaaaaa ccgccctgtg cttccggtat gtcaatttcg accggtgcct
    10201 gcgtttgcgg tgtttcacgc acccattttt gcaccagcgt cgcaatcagc aacaccatca
    10261 ccagcccggc caccagacga atcgccgcaa aaccccagcc gaggacaaac cccataaaca
    10321 ccagcgtcgc cgggtttaac accggattgc ccatccagaa tgccagcgca ccgcccatcg
    10381 acacctgttg gcgacgcatt cccgccgcga ccggagccgc acagcaggta cacatcatgc
    10441 ccggcagcga aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc ccaagcgtac
    10501 gcaacaacca gtcacgcggg atcagcacct gaatcaacga accgaggatc acccccagca
    10561 ctgccgcttt ccataccgcg aggaaataga gcatcgcgta atccaacgcc gcctgccacg
    10621 gattagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg gcagcagtaa
    10681 acgctttgcc gtagtaaggt tgccatttca cataccagag accggcaatg acaacgagaa
    10741 agaaaagcgc gggtttccac cactgaatgg gcgttgccgc ctgagatgaa gactgaccag
    10801 tcatagcatt ccccaggaat aagttatgat aattgagcgc gtgaatatta cgctcactat
    10861 caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt aaaatcgtca
    10921 caccttcatg cccgggccgc atcgcctctg ccagcatgac ccgcgccacg tctctcgcat
    10981 caatggattt ccagttaccg ggtaataaac ggaacagcgg cgcaaaaagc gtttcgttca
    11041 tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc aatttcggcc
    11101 agttctgggc gattaatgct tcttccatct cccctttgac gcggttatag aaaaacggcg
    11161 agtgggcatt ggcacccata gcactgacca ccagcatatg ctgcgcgccc agtcgccgcc
    11221 cggttaatgc ggtatccact accagcgtgt aatcggcatg aataaacgcc tctttgctcc
    11281 ccgcttctcg ccgcgtggtg ccgagacaac aaaacacaat gtcgatggga tcggtgacct
    11341 gcgccagcgc gtcagtcagt tgcggatcat ggggattaaa caccccgggc atatcgccca
    11401 acggacgtcg cgtcggcgcg gctatggcgt taactttcgg ttcgttaatc aacatccgca
    11461 gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag tacctgactc atcttcgctc
    11521 ctttacagaa ttgtctgcct tgcgctccac ggctcatgca ccaggcttaa tagaccatga
    11581 ggtaattatc cccgattgtg ggaaattcgc ctcatccaat gcaacaacgc ggaggaagca
    11641 tgagtaagaa aattgccgtt ttaatcactg atgaatttga agattcagaa tttacttcac
    11701 ccgcagacga gttccgtaaa gccggacacg aagtgattac cattgaaaaa caagcgggta
    11761 aaacggtgaa aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc atcgatgaag
    11821 tgacgccagc ggagtttgat gccctgctgc taccgggcgg ccattcaccg gattatctgc
    11881 gtggcgacaa ccgttttgtc acctttaccc gtgattttgt gaatagcggc aaacctgttt
    11941 tcgccatctg tcacggtccg cagctgctga tcagcgccga tgtgattcgg gggcgcaaac
    12001 tgaccgcggt taaaccgatc attattgatg ttaaaaacgc gggcgcggaa ttttacgatc
    12061 aggaagtcgt ggtcgataaa gatcagctgg ttaccagccg gacaccggac gatctgccag
    12121 cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagc cgcgtagcca aatcattttt
    12181 ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat ttcccacacc
    12241 ggggccgaca gcattctggc aaccggaaag cgacgattgt acgccttgcg cgcgaggtcg
    12301 ctttcctcac cttccagcct gcggatctca cctttaaact gcacgccgcg aattaacgct
    12361 accgttttcg gctgaccgtt taccgttccg gcaaccgcag cctgcggccc actcatctgc
    12421 gcgtggcgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc atcaaacaga
    12481 taaaaagcat tagcgcacca gagttcccct tcctgctgca cacaccaggt gacaacatgt
    12541 tgttttgcca accaacggct gatggtgatg agtgtttcca ttgctgttct cttttatact
    12601 gtgggcctga ccttaacatg cgacgcagac gatgacaccc tggtttcttt acttgatccg
    12661 taccgccgac aataagcttt ataccgggat caccacggat gtcgaacgcc gctatcagca
    12721 gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga cgctggcgtt
    12781 ttctgcgcca gtgggcgatc gttcgctggc gttacgggcg gaatatcgcg ttaagcaact
    12841 gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca gggtttgcag agctgttaag
    12901 cagtctgcaa accccggaga ttaaaagcga ttgaaatgct cgtgatactc aaccagacca
    12961 gttacgccat tcaacgcgtc atccgctaaa cgatgtacct ggaaggcgct ttcagtgcct
    13021 ggccagcggc aacgcaggtc gtgatgtgcc gccagttcaa agccaaaacg gctgtacagc
    13081 gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga atcgagtcct
    13141 tcatagacca gttggcgtgc cagcccctgt ccacggtatt tttcatcaac cgccaacggt
    13201 gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa tgccacatag
    13261 ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa gccatcttca
    13321 cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgagcgacg tagcagggca
    13381 tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca cctaccgatg
    13441 tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc agttgcagca
    13501 gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc aggtttttca
    13561 catacagccc cagctccgta tcgccttcaa tcaccagccg acgctggaag aagagcgtat
    13621 ccggatcttg tttacgcgcc gcaatcatca gcagatcgct ggcgtcggca ctaaaactca
    13681 catcagcttg cgcgttctgg ctgaccacca gtttgccatt caccaccgag gtaaaccatt
    13741 gcaggtcaat atcacgcaca tgaatactta accagcggcc ttcaagaaac tccagctcgc
    13801 catcatccag cgcctgacgg aattgccagc ttaagacctg ctcaagaacc tggcgtttta
    13861 gcgcaaatgg cgtcagtttt accggtacac tcaacagaga tggccccaaa tgcacaatac
    13921 gggaacgcag tttatccaac acgagtttta ctccctgttt caacaatcat cctattttgc
    13981 catatcagaa aaataacata gcggtataaa tcaacaattc catatgaaat tgctgctacc
    14041 accaatacaa ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta actaaaatcc
    14101 cagttcgtta acatttttgc gttttgatag cgcaaccttc aggaaaaatt atggagctgc
    14161 tctgccctgc cggaaatctc ccggcgctta aggcggccat cgaaaacggc gcagatgctg
    14221 tttatatcgg gctaaaagat gataccaatg cccgtcactt cgccggcctt aactttaccg
    14281 agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa cttcacatcg
    14341 cgattaacac ttttgcgcat ccggacggtt acgcccgttg gcagcgtgcc gtggatatgg
    14401 cggcgcagct gggtgccgac gcgctgatcc tcgccgacct cgccatgcta gagtacgccg
    14461 ccgagcgtta cccgcatatt gagcgtcatg tgtcagtgca ggcttcggcg accaatgaag
    14521 aggcgattaa cttttatcat cgccattttg acgttgctcg cgtggtgctg ccgcgcgtgt
    14581 tgtcgattca tcaggtgaaa caactggcac gggtcacacc tgtaccactg gaagtctttg
    14641 ctttcggcag cctgtgcatt atgtcggaag gtcgttgcta tctgtcgtcg tatctgacgg
    14701 gtgagtcgcc caacaccgta ggcgcgtgtt ctccggcccg tttcgtgcgc tggcaacaaa
    14761 cgccgcaggg gctggaatcc cgcctgaacg aagtgctgat cgaccgttat caggacggcg
    14821 aaaacgcagg ttatccgacg ctatgtaaag ggcgttatct ggtggacggc gagcgctatc
    14881 acgcgctgga agaaccaacc agtctcaata ccctggaact gctgccggag ttaatggcgg
    14941 cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat gtcagccagg
    15001 tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa aacttcatac
    15061 cgcaaagcgc gtggatggag acgctcgggt cgatgtccga aggcacgcag accacccttg
    15121 gcgcgtatca ccgtaaatgg cagtgagaag agcaatgaaa tattccttag ggccagtgct
    15181 gtggtactgg ccaaaagaga cgctggaaga tttttatcag caggccgcca ccagcagcgc
    15241 cgacgtgatt tatcttggtg aagcggtatg cagcaagcgc cgggcaacca aagttggcga
    15301 ctggctggag atggcaaaat cgctcgccgg aagtggcaag cagattgtgc tctccacgct
    15361 ggcgctggtg caggcatcct ctgaactggg cgaactgaaa cgctatgttg agaacggtga
    15421 gtttctgatt gaagccagcg atctcggcgt ggtgaatatg tgcgctgaac gcaaactgcc
    15481 gttcgtcgcc gggcacgcgc tgaactgcta caacgcggtg acgctgaaaa tattgctcaa
    15541 acagggcatg atgcgctggt gtatgccggt ggagctttcc cgcgactggc tggtgaatct
    15601 gcttaatcag tgcgatgagc tgggcattcg caaccagttt gaagtggaag tcctgagcta
    15661 cggtcatcta ccgctggcct actccgcccg ctgctttacc gcgcgttcgg aagaccgccc
    15721 gaaagatgag tgtgaaacct gctgcattaa gtatccgaac gggcgcaacg tgctgtcgca
    15781 ggaaaaccaa caagtgtttg tactcaatgg cattcagacc atgagcggct acgtttacaa
    15841 cctcggtaac gagctggcat ccatgcaggg gctggttgat gtggtccgcc tgtcaccgca
    15901 gggtactgac actttcgcga tgctcgacgc cttccgcgct aatgaaaatg gcgctgcgcc
    15961 actaccgttg acggcaaaca gcgactgtaa cggctactgg cggcggctgg ccgggctgga
    16021 gctgcaagcc taagtaaata tgctcgtcat acttcaagtt gcatgtgctg catctgcatt
    16081 cgttcacccc agtcacttac ttatgtaagc tcctggggat tcactctctt gtcgccttcc
    16141 tgcaactcga attatttaga gcacagctca ctttgttaac aactttaact actctttaat
    16201 gcagtattaa agattaatcg gtaacaaagt gagctgttat gactgataaa accattgcgt
    16261 tttcgctact cgatctggcc cccattcccg aaggttcttc agcgcgagaa gcattctccc
    16321 actctctcga tctcgcccgt ctggctgaaa agcgcggcta tcatcgctac tggctggcag
    16381 aacaccacaa tatgactggc attgccagtg ctgccacgtc ggtattgatt ggctatctgg
    16441 cggcgaatac caccacgctg catctggggt ctggcggcgt gatgttgcct aaccactcac
    16501 cgttggtcat tgccgaacag ttcggcacgc tcaatacact ctatccgggg cgaatcgatt
    16561 tggggctggg tcgtgcgcca ggtagtgacc aacggacaat gatggcgctg cgtcgccata
    16621 tgagcggcga tattgataat ttcccccgcg atgtcgcgga gctggtggac tggtttgacg
    16681 cccgcgatcc caatccgcat gtgcgcccgg taccaggcta tggcgagaaa atccccgtgt
    16741 ggttgttagg ctccagcctt tacagcgcgc aactggcggc gcagcttggt ctgccgttcg
    16801 cgtttgcctc acacttcgcg ccggatatgt tgttccaggc gctgcatctt tatcgcagca
    16861 acttcaaacc gtcggcacga ctggaaaaac catacgcgat ggtgtgcatc aatattatcg
    16921 ccgccgatag caaccgcgac gctgagtttc tgtttacctc aatgcagcaa gcctttgtga
    16981 agttgcgccg tggcgaaacc gggcaactgc cgccaccgat tcaaaatatg gatcagttct
    17041 ggtcgccgtc cgaacagtat ggtgtgcagc aggcgctgag tatgtcgttg gtaggtgata
    17101 aagcgaaagt gcgtcatggc ttgcagtcga tcctgcgcga aaccgacgcc gatgagatta
    17161 tggtcaacgg gcagattttc gaccaccagg cgcggctgca ttcgtttgag ctggcgatgg
    17221 atgttaagga agagttgttg ggatagtgtg tcttaacgcg ggaagcctta tccgagctgg
    17281 caacgctgtc ct
//
LOCUS       DAZGLJ010000098        17269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000098 DAZGLJ010000000
VERSION     DAZGLJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.098"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            309..395
                     /gene="leuL"
                     /locus_tag="ACHK49_002735"
     CDS             309..395
                     /gene="leuL"
                     /locus_tag="ACHK49_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO9263356.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            488..2059
                     /gene="leuA"
                     /locus_tag="ACHK49_002736"
     CDS             488..2059
                     /gene="leuA"
                     /locus_tag="ACHK49_002736"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO9263357.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2059..3150
                     /gene="leuB"
                     /locus_tag="ACHK49_002737"
     CDS             2059..3150
                     /gene="leuB"
                     /locus_tag="ACHK49_002737"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO9263358.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3153..4553
                     /gene="leuC"
                     /locus_tag="ACHK49_002738"
     CDS             3153..4553
                     /gene="leuC"
                     /locus_tag="ACHK49_002738"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO9263359.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4564..5169
                     /gene="leuD"
                     /locus_tag="ACHK49_002739"
     CDS             4564..5169
                     /gene="leuD"
                     /locus_tag="ACHK49_002739"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO9263360.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5218..6396)
                     /gene="setA"
                     /locus_tag="ACHK49_002740"
     CDS             complement(5218..6396)
                     /gene="setA"
                     /locus_tag="ACHK49_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFO9263361.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6498..6629)
                     /gene="sgrT"
                     /locus_tag="ACHK49_002741"
     CDS             complement(6498..6629)
                     /gene="sgrT"
                     /locus_tag="ACHK49_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO9263362.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWDDRNSES"
     gene            6718..8373
                     /gene="sgrR"
                     /locus_tag="ACHK49_002742"
     CDS             6718..8373
                     /gene="sgrR"
                     /locus_tag="ACHK49_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO9263363.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLILTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8407..8506
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8537..9520
                     /gene="thiB"
                     /locus_tag="ACHK49_002743"
     CDS             8537..9520
                     /gene="thiB"
                     /locus_tag="ACHK49_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO9263364.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9496..11106
                     /gene="thiP"
                     /locus_tag="ACHK49_002744"
     CDS             9496..11106
                     /gene="thiP"
                     /locus_tag="ACHK49_002744"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO9263365.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11090..11788
                     /gene="thiQ"
                     /locus_tag="ACHK49_002745"
     CDS             11090..11788
                     /gene="thiQ"
                     /locus_tag="ACHK49_002745"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO9263366.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11902..12666)
                     /gene="yabI"
                     /locus_tag="ACHK49_002746"
     CDS             complement(11902..12666)
                     /gene="yabI"
                     /locus_tag="ACHK49_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO9263367.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12752..13630)
                     /gene="araC"
                     /locus_tag="ACHK49_002747"
     CDS             complement(12752..13630)
                     /gene="araC"
                     /locus_tag="ACHK49_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO9263368.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRYPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13969..15669
                     /gene="araB"
                     /locus_tag="ACHK49_002748"
     CDS             13969..15669
                     /gene="araB"
                     /locus_tag="ACHK49_002748"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO9263369.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15680..17182
                     /gene="araA"
                     /locus_tag="ACHK49_002749"
     CDS             15680..17182
                     /gene="araA"
                     /locus_tag="ACHK49_002749"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO9263370.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
BASE COUNT         4128 a         4746 c         4589 g         3806 t
ORIGIN      
        1 tcaaaaaaaa atcgcaaaac atataattca atacaaatca tcaggatagg ttttgcaacg
       61 cgtgcatttt gtcccctttt tcctcgttga ttagatgcaa aaatttatgc tgaaatatgt
      121 caaccgatga aaagcgtcgg tagttaagca gaaattaata tcgcttactt taaccaccgc
      181 agcacaatta gctaatttta cgggtgcaga actcacgctg gcgggacgtt tttattgcgt
      241 cagggttgac atccgttttt gtatccagta actctaaaag catatcgcat tcatctggag
      301 ctgatttaat gactcacatc gttcgcttta tcggtctact actactaaac gcatcttctt
      361 tgcgcggtag acgagtgagc ggcatccagc attaagccag cacgcagtca aacaaaaaac
      421 ccgcgccatt gcgcgggttt ttttatgccc gaagcgaggc gctctaaaag agacaaggac
      481 ccaaaccatg agccagcaag tcattatttt cgataccaca ttgcgcgacg gtgaacaggc
      541 gttacaggca agcttgagtg tgaaagaaaa actgcaaatt gcgctggccc ttgagcgtat
      601 gggtgttgac gtgatggaag tcggtttccc cgtctcttcg ccgggcgatt ttgaatcggt
      661 gcaaaccatc gcccgccagg ttaaaaacag ccgcgtatgt gcgttagctc gctgcgtgga
      721 aaaagatatc gacgtggcgg ccgaatccct gaaagtcgcc gaagccttcc gtattcatac
      781 ctttattgct acctccccaa tgcacatcgc caccaagctg cgcagcaccc tggacgaggt
      841 gatcgaacgc gctatctata tggtgaaacg cgcccgtaat tacaccgatg atgttgaatt
      901 ttcttgcgaa gatgccgggc gtacacccat tgccgatctg gcgcgagtgg tcgaagcggc
      961 gattaatgcc ggtgccacca ccatcaacat tccggacacc gtgggctaca ccatgccgtt
     1021 tgagttcgcc ggaatcatca gcggcctgta tgaacgcgta cctaacatcg acaaagccat
     1081 tatttccgta catacccacg acgatttagg cctggcggtc ggcaactcac tggcggcggt
     1141 acatgccggt gcacgccagg tggaaggtgc aatgaacggg atcggcgagc gtgccggtaa
     1201 ctgttcgctg gaagaagtca tcatggcgat taaagttcgt aaggatattc tcaacgtcca
     1261 caccgccatt aatcaccagg aaatatggcg caccagccag ttagttagcc agatttgtaa
     1321 tatgccgatc ccggcaaaca aagccattgt tggcagcggc gcattcgcac actcctccgg
     1381 tatccaccag gatggcgtgc tgaaaaaccg cgaaaactac gaaatcatga caccagaatc
     1441 tattggtctg aaccaaatcc agctgaatct gacctctcgt tcggggcgtg cggcggtgaa
     1501 acatcgcatg gacgagatgg ggtataaaga aagtgaatat aatttagaca atttgtacga
     1561 cgccttcctg aagctggcgg acaaaaaagg tcaggtgttt gattacgatc tggaggcgct
     1621 ggccttcatc ggtaagcagc aagaagagcc ggagcatttc cgtctggatt acttcagcgt
     1681 gcagtctggc tctaacgata tcgccaccgc cgccgtcaaa ctggcctgtg gcgaagaagt
     1741 caaagcagaa gccgccaacg gtaacggtcc ggtcgatgcc gtctatcagg caattaaccg
     1801 catcactgac tataacgtcg aactggtgaa atacagcctg accgccaaag gtcacggtaa
     1861 agatgcgctg ggtcaggtgg atattgtcgc caactacaac ggtcgccgct tccacggcgt
     1921 cggcctggcc accgatattg tcgagtcctc cgccaaagcc atggtgcacg tactgaacaa
     1981 tatctggcgt gccgcagaag tcgaaaaaga gttgcaacgc aaagctcaac acaacgaaaa
     2041 caacaaggaa accgtgtgat gtcgaagaat taccatattg ccgtattgcc gggggacggt
     2101 attggtccgg aagtgatgac ccaggcgctg aaagtgctgg atgccgtgcg caaccgcttt
     2161 gcgatgcgca tcaccaccag ccattacgat gtaggcggtg cagccattga taaccacggg
     2221 caaccactgc cgcctgcgac ggttgaaggt tgtgagcaag ccgatgccgt gctgtttggc
     2281 tcggtaggcg gtccgaaatg ggaacattta ccaccagacc agcaaccaga acgcggcgcg
     2341 ctgttgcctt tgcgtaagca cttcaaatta ttcagcaacc tgcgcccggc aaaactgtat
     2401 caggggctgg aagcattctg tccgctgcgt gcagacattg ccgctaacgg cttcgacatc
     2461 ctgtgcgtgc gcgaactgac cggcggcatc tatttcggtc agccaaaagg ccgcgaaggt
     2521 agcggacaat atgaaaaagc gtttgatacc gaggtgtatc accgttttga gatcgagcgt
     2581 attgcccgca tcgcgtttga atctgctcgt aaacgtcgcc acaaagtcac ctcaatcgac
     2641 aaagctaacg tgctgcaatc ctctatttta tggcgtgaaa tcgtcaacga gatcgccacg
     2701 gaatacccgg atgtcgaact ggcgcatatg tacatcgaca acgccaccat gcagctgatt
     2761 aaagatccat cacagtttga cgttctgctg tgctccaacc tgtttggcga cattctgtct
     2821 gacgagtgcg caatgatcac tggctcgatg gggatgttgc cttccgccag cctgaacgag
     2881 caaggttttg gtctgtatga accggcaggc ggctcagcac cagatatcgc aggcaaaaac
     2941 atcgccaacc cgattgcgca aattctgtcg ctggcactgc tgctgcgcta cagcctggat
     3001 gccgatgatg cggcttgcgc cattgaacgc gccattaacc gcgcattaga agaaggcatt
     3061 cgcaccgggg atttagcccg tggcgctgcc gccgttagta ccgatgaaat gggcgatatc
     3121 attgcccgct atgtggcaga aggggtgtaa tcatggctaa aacgttatac gaaaaattat
     3181 tcgacgctca cgtagtgtac gaagccgaaa acgaaacccc gctgttatat atcgaccgcc
     3241 acctggtgca tgaagtgacc tcaccgcagg cgttcgatgg tctgcgcgcc cacggtcgcc
     3301 cggtacgtca gccgggcaaa accttcgcca ctatggatca caacgtctct acccagacga
     3361 aagacattaa tgcctgtggt gaaatggcgc gcatccagat gcaggagctg attaaaaact
     3421 gcaaagaatt tggcgtcgaa ctgtatgacc tgaatcaccc gtatcagggg atcgtccacg
     3481 taatggggcc ggaacagggc gtcaccttgc cggggatgac cattgtctgc ggcgactcgc
     3541 ataccgccac ccacggcgcg tttggcgcac tggcctttgg tatcggcact tccgaagttg
     3601 aacacgtact ggcaacgcaa accctgaaac agggccgcgc aaaaaccatg aaaattgaag
     3661 tccagggcaa agccgcgccg ggcattaccg caaaagatat cgtgctggca attatcggta
     3721 aaaccggtag cgcaggcggc accgggcatg tggtggagtt ttgcggcgaa gcaatccgtg
     3781 atttaagcat ggaaggtcgt atgaccctgt gcaatatggc aatcgaaatg ggcgcaaaag
     3841 ccggtctggt tgcaccggac gaaaccacct ttaactatgt caaaggccgt ttgcatgcgc
     3901 cgaaaggcaa agatttcgac gacgccgttg cctactggaa aaccctgcaa accgacgaag
     3961 gcgcaacttt cgataccgtt gtcactctgc aagcagaaga aatttcaccg caggtcacct
     4021 ggggcaccaa tccaggccag gtgatttccg tgaacgacaa tattcccgat ccggcttcgt
     4081 ttgccgatcc ggttgaacgc gcgtcggcag aaaaagcgct ggcctatatg gggctgaaac
     4141 cgggcattcc gctgaccgaa gtggctatcg acaaagtgtt tatcggttcc tgtaccaact
     4201 cgcgcattga agatttacgc gcggcagcgg agatcgccaa agggcgaaaa gtcgcgccag
     4261 gcgtgcaggc actggtggtt cccggctctg gcccggtaaa agcccaggcg gaagcggaag
     4321 gtctggataa aatctttatt gaagccggtt ttgaatggcg cttgcctggc tgctcaatgt
     4381 gtctggcgat gaacaacgac cgtctgaatc cgggcgaacg ttgtgcatcc accagcaacc
     4441 gtaactttga aggccgccag gggcgcggcg ggcgcacgca tctggtcagc ccggcaatgg
     4501 ctgccgctgc tgctgtgacc ggacatttcg ccgacattcg caacattaaa taaggagcac
     4561 accatggcag agaaatttat caaacacaca ggcctggtgg ttccgctgga tgccgccaat
     4621 gtcgataccg atgcaatcat cccgaaacag tttttgcaga aagtgacccg tacgggtttt
     4681 ggcgcgcatc tgtttaacga ctggcgtttt ctcgatgaaa aaggccaaca gccaaacccg
     4741 gacttcgtgc tgaacttccc gcagtatcag ggcgcttcca ttttgctggc acgagaaaac
     4801 ttcggctgtg gctcctcgcg tgagcacgcg ccctgggcat tgaccgacta cggttttaaa
     4861 gtggtgattg cgccgagttt tgctgacatc ttctacggca atagctttaa caaccagctg
     4921 ctgccggtga aattaagcga tgcagaagtg gacgaactgt ttgcgctggt gaaagccaat
     4981 ccggggatcc atttcgacgt ggatctggaa gcgcaagagg tgaaagcggg agagaaaacc
     5041 tatcgcttta ccatcgatgc cttccgccgc cactgcatga tgaacggtct ggacagtatt
     5101 gggcttacct tgcagcacga cgacgccatt gccgcttatg aagcaaaaca acctgcgttt
     5161 atgaattaat ccccttgccc ggtcaaatga ccgggctttc cgctatcgtc cacgtcatca
     5221 aacgtcttta acctttgcgg ttaaaaataa tgcgacaaca gaaataaccg caattaccca
     5281 gtagacagca aagtgccccc aactttgtgc aattgctccc tgaataacgc cagccagaat
     5341 taccccggta gaaatactgt tagtaaataa ggtggtagct gcccccgctc ttccaggcat
     5401 taaatcctga aaccatagca tcccaatacc cgcaacaatg ccgataaata cagcgttaaa
     5461 aagttgcagc gtcatcaacg ccatacggct atgaaagaaa atcaatccgg tgtaaaacag
     5521 tactcctgcc gccactgcta tgaccatcat tcgccgctta ccataacgtt tgacatagta
     5581 gccagccaga atcattgctg gtatttccag tccagctgcc gtccccatca ggaaacccgc
     5641 cagtttgtct ggcaatccta actcgctact gatccacaac ggcatatcaa taatgtacat
     5701 ggtgttgcag gtccacatta acgtcgaggc gacaaataac atccgtacgt tactatcctg
     5761 ccagccacct tgcattgata aagcattttc cgacggcagt tctacccgcg ccacagacgg
     5821 aagcataaat gcaatcaata ccagactgag tgtgaatatc ccggcggcaa tcgaaaacat
     5881 caccgtaaag ccgtaattca acgccagcat aaaggccaac ggtggaccga taacccatgc
     5941 cagagaaagc tgcgcacgca tcaccgagct aaacatcacc acttctcgcg ccgagttatc
     6001 cgcatattcc cgcgccagag caaataactg tggcattgcc gtattggcca gagatgccag
     6061 aagcacacca caggtgataa gcgtcagata atgacgatta aatgcaaaca atagcgcatt
     6121 gccgatagcc atcaaacagc aaaatataat cagttttcgc cgatcgccct gactgtcaga
     6181 acgttttgcc aaccagaggc ttacgccgat cccagcaata gcattcaccg tataaaagag
     6241 tccgatccag aaaggttgcg cgccaacctc acgactcaga aataagctca atgtaggagc
     6301 ctgtagcgcc ccggccaccc ccatcataaa agcgaccagc ataaatgccg cgtaaacacc
     6361 gttcatacgg cgagccatcg tcattatcca gatcatacgt tcccttttta gcgcggcgag
     6421 aataaaaaaa accagcaggt ataatctgct ggcgggtgat tttacaccaa tactcagtca
     6481 cacatgatgc aggcaagtca actttcagaa ttgcggtcat cccactgcat cagttcttca
     6541 agcatggtta atcgttgtgg gacgcttaac caacgcaacc agcacaactt cgctgtcgcg
     6601 gtaaaatagt gctgataaaa ctgacgcatg gggcacctcc ttgcttcatc gttaggaatt
     6661 attgacttaa tatagggaaa ataaaattgc tgtcttttgc gcaggagttc cccttttatg
     6721 ccatctgctc gtctgcaaca acagttcatc cgcctgtggc aatgctgcga gggtaaatcg
     6781 caggacacaa cgctcaacga actggcagcg ttattgagct gctcgcgtcg tcatatgcgt
     6841 accctgctca acaccatgca ggatcgcggc tggctgacgt gggaagcgga agtcgggcgc
     6901 ggtaaacgct cgcgtctgac attcctctat accgggctgg cgcttcagca acagcgggcg
     6961 gaagacctgc tggagcagga tcgtatcgac caactagtgc agttggttgg cgacaaagcg
     7021 actgtgcggc aaatgctggt ttctcatctg ggccgcagct tccgccaggg gcggcacatc
     7081 ctgcgcgtgc tctactatcg tccgttgcgt aatctgctac ctggcagcgc attgcgccgt
     7141 tccgaaaccc atattgcccg gcaaatcttc agttcgctaa cgcgcataaa tgaggaaaat
     7201 ggggaactgg aagcagacat cgcccaccac tggcagcaaa tatcaccgct tcactggcgt
     7261 ttctttttgc gtccaggagt ccattttcat catggtcgtg aactggaaat ggacgacgtg
     7321 atcgcctctt taaaacgaat caatacgctg ccgctctatt cgcatattgc ggacattgtg
     7381 tcgccgacgc cctggacgct ggatatccat ctcacgcaac cggaccgctg gttaccgtta
     7441 ctgctgggac aagttccggc gatgatcctg ccgcgcgaat gggaaaccct cagtaacttt
     7501 gccagccatc ccatcggcac cggtccgtat gcggtgatcc gcaacaccac caatcaactg
     7561 aaaattcagg cattcgatga cttcttcggt taccgggcat taatcgatga agttaacgtc
     7621 tgggttctgc cggaaattgc cgacgagcca gctggaggtc tgatgctaaa aggtccacag
     7681 ggtgaggaaa aagagattga aagccgcctg gaggaaggtt gctactattt actgttcgac
     7741 agccgcaccc atcgcggggc gaatcagcaa gtcagggact gggtaagcta tgtgctttct
     7801 ccaactaatc tggtctattt cgctgaggaa cagtaccagc aactgtggtt cccggcttat
     7861 ggactgctcc cccgttggca ccatgcccgc accataaaga gcgaaaaacc ggctggcctg
     7921 gaaagcctca tcctgacctt ttatcaggat cacagtgagc atcgggtgat tgccgggatc
     7981 atgcagcaga ttctggcaag tcaccaggtc acgctggaaa tcaaagagat cagctacgat
     8041 cagtggcatg aaggagagat cgagagcgat atctggctta acagcgccaa ctttacgctg
     8101 cctctggatt tttcgctgtt cgcgcacttg tgcgaggtgc cgctgctcca acactgtatt
     8161 ccgatcgact ggcaagccga cgccgcccgc tggcgcaatg gcgaaatgaa cctggcgaac
     8221 tggtgccagc aactggtcgc cagcaaagca atggtgccgc ttatccacca ctggctgatc
     8281 attcaggggc aacgcagtat gcgcggcctg cgcatgaaca ccctcggctg gttcgatttt
     8341 aaatcagcgt ggtttgcgcc accggatcca tgattgctgg tagataacaa aatcactaca
     8401 ctaacgccgt tctcaacggg gtgccacgca aacgcgtgcg ctgagaaaat acccgtcgaa
     8461 cctgatccgg ataacgccgg cgaagggatt tgaggctcct tctcaagtcc tttgccactc
     8521 ttttttgagg tgcaaagtgt taaaaaaatg tcttcccctg ctgttgctgt gcacagcgcc
     8581 cgttttcgct aaacccgttc tgactgttta tacctacgat tccttcgccg ccgactgggg
     8641 gcctggtcca aagatcaaaa aagcttttga agccgactgt aactgcgaac tgaaactggt
     8701 ggcgctggaa gatggcgttt cgcttctcaa ccgtctacgg atggaaggca aaaatagtaa
     8761 agccgatgtg gtgctggggc tggataacaa tctgttagac gccgccagta aaaccggact
     8821 atttgccaaa agcggtgtgg cagcggatgc cgttaacgtt cccggcggct ggaataatga
     8881 cactttcgtc ccgtttgatt acggctattt cgccttcgtc tatgacaaga acaaactgaa
     8941 aaacccgccg caaagcctga aagaattggt tgagagcgat cagaactggc gggtgattta
     9001 tcaggatccg cgcaccagta cgccggggct gggcctgctg ttgtggatgc aaaaagtcta
     9061 tggcgatgac gccccacaag cctggcaaaa actggcgaag aaaaccgtca cggtcaccaa
     9121 aggctggagc gaagcctacg gcctgttttt aaaaggtgaa agcgatctgg tactgagtta
     9181 caccacctct ccggcttatc acattctcga agagaaaaaa gataactacg ccgccgcgaa
     9241 ctttagcgaa ggtcactatc tgcaggtgga agtcgccgcc cgcaccgctg ccagcaagca
     9301 gccggagctg gcgcaaaaat tcctccagtt tatggtttct ccggcttttc agaatgcgat
     9361 cccaaccggt aactggatgt atccggtggc aaatgtcacg ctgcctgccg ggtttgaaca
     9421 attgaccaaa ccggccacca cgctggagtt cacaccagct gaagtggctg cacaacgtca
     9481 ggcatggatt agcgaatggc aacgcgccgt cagccgttaa ttcccggctg gttaattcca
     9541 ggtgtaagcg ccgccacgct ggtggtagcg gttgcgctcg cggcgtttct cgccctgtgg
     9601 tggaacgcgc cgcagggtaa ctgggtggca gtctggcagg acagctacct gtggcatgtg
     9661 gtgcgcttct ccttctggca ggcgtttctc tcggcgctac tctctgtcgt acccgcgata
     9721 ttcctcgccc gcgccctcta tcgcaggcgc tttccgggtc ggctggcgct gttgcgtctg
     9781 tgcgcaatga ccttgatcct cccggtgcta gttgctgttt tcggcattct tagcgtctat
     9841 ggtcgtcagg gctggctggc atcgctctgc caatcgctcg gtctggagtg gaccttttcg
     9901 ccctacggcc tgcaaggcat tttgctggcg cacgtatttt ttaatctgcc gatggcgagc
     9961 cgcttattac tccaggcact ggaaaacatt cccggcgaac aacgtcagct tgccgcccag
    10021 cttgggatgc gcggctggca ttttttccgc ttcgtcgaat ggccgtggct acggcgacaa
    10081 atcccgccgg ttgccgcgct tatctttatg ctctgtttcg ccagcttcgc caccgtgcta
    10141 tcgctgggcg gcggtccgca ggcaactact atcgagctgg ctatttatca ggcgctgagt
    10201 tacgactacg atcctgcccg cgcggcgatg ctggcgctgc tccagatggt gtgttgcctt
    10261 gggctggtgc tgttgagtca gcgactaagt aaggccattg cgcccggtac cacgctgctg
    10321 caaggctggc gcgatccgga cgatcgtctg catagccgca tttgcgacac ggtgttaata
    10381 gtgctggcgc tgctgctgtt gctgccaccg ttgctggcgg tgatcgtcga tggggtaaat
    10441 cgccagttgc cggaagtgct ggcacaaccg gtgctgtggc aggcgctgtg gacctcgttg
    10501 cgtattgcgc tggcggcagg tgtattgtgc gtagtgctga ccatgatgct gctatggagc
    10561 agtcgcgaac tgcgggcgcg gcagaaaatg ctggcgggtc aggcgctgga gatgagcggc
    10621 atgttgatcc tcgccatgcc ggggattgtg ctggctaccg gcttcttttt actgctcaac
    10681 aacactatcg gcctgccaca atctgctgac ggcattgtga ttttcaccaa tgcgttaatg
    10741 gcgatccctt atgcgctgaa agtactggaa aacccgatgc gcgatatcac cgcccgctac
    10801 agtatgttgt gtcagtcgct gggcattgaa ggctggtcgc gcttaaaagt ggtcgagctg
    10861 cgcgccctga aacgtccact ggcgcaggcg ctggcttttg cctgcgtgct gtcgattggt
    10921 gattttggcg tagtggcgtt gttcggtaac gatgatttcc gtaccctgcc gttttatctc
    10981 taccagcaaa ttggctccta tcgcagccag gacggcgcgg tcaccgcgtt aattctgcta
    11041 ctactctgtt ttctgctgtt taccgtgatt gaaaaactac cggggcgaaa tgttaaaact
    11101 gactgatatc acctggcttt atcaccattt gccgatgcgt tttagcttaa cggtagaacg
    11161 cggcgagcag gtggcaatcc tcgggccaag cggcgcgggt aaaagtaccc tgctgaattt
    11221 gattgccggt tttctgacgc cagccagcgg ttcgctgact atcgacagcg ttgatcacac
    11281 cacgacgccg ccgtcacgcc gcccggtatc gatgctgttt caggagaaca acctgttcag
    11341 ccacctgacg gtcgcgcaga acatcgggct ggggcttaat ccgggattga agctgaacgc
    11401 ggcgcagcag gagaaaatgc acgctatcgc ccgccagatg gggattgata atttaatggc
    11461 gcggttaccg ggcgagcttt ccggcggtca gcgacagcgc gtggcgttag cgcgttgtct
    11521 ggtgcgcgaa cagccgattt tattgctcga tgaaccgttc tctgcgctcg atccggcgtt
    11581 acgtcaggag atgctgacgc tggtgagcac gagctgccag cagcaaaaaa tgacgctatt
    11641 gatggtgtcg cacagtgtgg aagatgcggc gcggatcgcc acacgctcgg tggtggtcgc
    11701 cgacgggcgc atcgcctggc agggtaagac caatgagttg ttgagcggta aggcgagtgc
    11761 ttcggcacta ttggggatta cgggttagta tgcactgtcg gatgcggcgt gaacgcctta
    11821 tccgacctac gggtagtgcg ttttgtaggc ctgataagac gcggtcagcg tcgcatcagg
    11881 caacccgcac aagactatct cctaaccccc aaccacttta cgcaaaatat cgatatacac
    11941 cggcatcaac gggtggcgaa ttaacaccac cagcgccacc acgccgatgg cagaaattaa
    12001 cggcgtcagc cacaacaaac ggccgcgcga caaataatga cttaaacgat cggttgcttt
    12061 accgctgcgc cataaccgcc agcacagcca gccaccaacc cacaaaaata ccgccgtcgc
    12121 cagcagcaac catttaaact cgccgctctg cattccggct ggaatatcga tcgccgcgcc
    12181 cgccagaatc ccaggcagga agtaaaacgg tggccacagc aggcagccga taatattcgg
    12241 cgtaatgaat tttgccaccg gcagatccag cattcccgcc accattggca ccagcggacg
    12301 cgtcgggcca acaaagcgac cgaccagaat ggtgaacatg ctgtgttgat gcaacgcatg
    12361 ttcagtttta tcgagtaatg ctttgttttt cttcagaaat gaccagcgat gcaacggctt
    12421 tttaaaacgc cagcccagcc agaaggagat ccagtcgccc agcaggcaac cgacgatccc
    12481 tgccagccag gcgtgccaga aacttaactc gccgctgcca atcagcgctc ccagccccgc
    12541 catcagcacc gtaccgggta gaatcaaacc gaccagcgcc agcgactcca gaaaggccac
    12601 caacaccacc gccatcaatg aatacacggt agattgggta ataaagtgtt ccagcaatgc
    12661 ttgcataatg tgcctgtcaa atggacgaag cagggattct gcaaacccta tgctactccg
    12721 tcaagccgtc aattgtctga ttcgttacca attatgacaa cttgacggct acatcattca
    12781 ctttttcttc acaaccggct cggaactcgc tcgggctggc cccggtgcat tttttaaata
    12841 cccgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg gcgataggca
    12901 tccgggtggt gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg cgccagctta
    12961 agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa
    13021 catgctgtgc gacgctggcg atatcaaaat tgctgtctgc caggtgatcg ctgatgtact
    13081 gacaagcctc gcgtacccga ttatccatcg gtggatggag cgactcgtta atcgcttcca
    13141 tgcgccgcag taacaattgc tcaagcagat ttatcgccag cagctccgaa tagcgccctt
    13201 ccccttgccc ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt
    13261 catccgggcg aaagaacccc gtattggcaa atattgacgg ccagttaagc cattcatgcc
    13321 agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgcgagcc tccggataac
    13381 gaccgtagtg atgaatctct cctggcggga acagcaaaat atcacccggt cggcaaacaa
    13441 attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagattg agaatataac
    13501 ctttcattcc cagcggtcgg tcgataaaaa aatcgagata accgttggcc tcaatcggcg
    13561 ttaaacccgc caccagatgg gcattaaacg agtatcccgg cagcagggga tcattttgcg
    13621 cttcagccat acttttcata ctcccgccat tcagagaaga aaccaattgt ccatattgca
    13681 tcagacattg ccgtcactgc gtcttttact ggctcttctc gctaacccaa ccggtaaccc
    13741 cgcttattaa aagcattctg taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca
    13801 aaagtgtcta taatcacggc agaaaagtcc acattggtta tttgcacggc gtcacacttt
    13861 gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc
    13921 aactctctac tgtttctcca tacccgtttt tttggatgga gtgaaacgat ggcgattgca
    13981 attggcctcg attttggcag tgattctgtg cgagctttgg cggtggactg cgccagcggt
    14041 gaagagatcg ccaccagcgt agagtggtat ccccgttggc aaaaagggca attttgtgat
    14101 gccccgaata accagttccg tcatcatccg cgtgactaca ttgagtcaat ggaagcggca
    14161 ctgaaaaccg tgcttgcaga gcttagcgtc gaacagcgcg cagctgtggt cgggattggc
    14221 gttgacagta ccggctcgac gcccgcaccg attgatgccg acggtaacgt gctggcgctg
    14281 cgcccggagt ttgccgaaaa cccgaacgcg atgttcgtat tgtggaaaga ccacactgcg
    14341 gttgaagaag cggaagagat tacccgtttg tgccacgcgc cgggcaacgt tgactactcc
    14401 cgctacattg gcggtattta ttccagcgaa tggttctggg caaaaatcct gcatgtgact
    14461 cgccaggaca ccgccgtggc gcaatctgcc gcatcgtgga ttgagctgtg cgactgggtg
    14521 ccagctctgc tttccggtac cacccgcccg caggatattc gtcgcggacg ttgcagcgcc
    14581 gggcataagt cgctatggca cgaaagctgg ggtggcttgc cgccagccag tttctttgat
    14641 gagctggacc cgatcctcaa tcgccattta ccttccccgc tgttcactga cacctggact
    14701 gccgatattc cggtgggcac cttatgcccg gaatgggcgc agcgtctcgg cctgcctgaa
    14761 agcgtggtga tttccggcgg cgcgtttgac tgccatatgg gcgcagttgg cgcaggcgca
    14821 cagcctaacg cactggtaaa agttatcggt acttccacct gcgacattct gattgccgac
    14881 aaacagagcg ttggcgagcg ggcagttaaa ggtatttgcg gtcaggttga tggcagcgtg
    14941 gtacctggat ttatcggtct ggaagcaggc caatcggcgt ttggtgatat ctacgcctgg
    15001 tttggtcgcg tactcggctg gccgctggaa cagcttgccg cccagcatcc ggaactgaaa
    15061 gcgcaaatca acgccagcca gaaacaactg cttccggcgc tgaccgaagc atgggccaaa
    15121 aatccgtctc tggatcacct gccggtggtg ctcgactggt ttaacggtcg tcgcacaccg
    15181 aacgctaacc aacgcctgaa aggggtgatt accgatctta acctcgctac cgacgctccg
    15241 ctgctgttcg gcggtttgat tgctgccacc gcctttggcg cacgcgcaat catggagtgc
    15301 tttaccgatc aggggatcgc cgtcaataac gtgatggcgc tgggcggcat cgcgcggaaa
    15361 aaccaggtca ttatgcaggc ctgctgcgac gtgctgaatc gcccgctgca aattgttgcc
    15421 tctgaccagt gctgtgcgct cggtgcggcg atttttgctg ccgtcgccgc gaaagtgcac
    15481 gcagacatcc catcagccca gcaaaaaatg gccagtgcgg tagagaaaac cctgcaaccg
    15541 cgcagcgaac aggcacaacg ctttgaacag ctttatcgcc gctatcagca atgggcgatg
    15601 agcgccgaac aacactatct tccaacttcc gccccggcac aggctgccca ggccgttgcg
    15661 actctataag gacacgataa tgacgatttt tgataattat gaagtgtggt ttgtcattgg
    15721 cagccagcat ctgtatggcc cggaaaccct gcgtcaggtc acccaacatg ccgagcacgt
    15781 tgttaatgcg ctgaatacgg aagcgaaact gccctgcaaa ctagtgttga aaccgctggg
    15841 caccacgccg gatgaaatca ccgctatttg ccgcgacgcg aattacgacg atcgttgcgc
    15901 tggtctggtg gtgtggctgc acactttctc cccggccaaa atgtggatca acggcctgac
    15961 catgctcaac aaaccgttgc tgcaattcca cacccagttt aacgcggcgc tgccgtggga
    16021 cagtatcgat atggacttta tgaacctgaa ccagactgca catggcggtc gcgagttcgg
    16081 cttcattggc gcgcgtatgc gtcagcaaca tgccgtggtt accggtcact ggcaggataa
    16141 acaagcccat gagcgtatcg gctcctggat gcgccaggcg gtctctaaac aggatacccg
    16201 tcatctgaaa gtctgccgtt ttggcgataa catgcgtgaa gtagcggtca ccgatggtga
    16261 taaagttgcc gcacagatca agttcggttt ctccgtcaat acctgggcgg ttggcgatct
    16321 ggtgcaggtg gtgaactcca tcagcgacgg cgatgttaac gcgctggtcg atgagtacga
    16381 aagctgctac accatgacgc ctgccacaca aatccacggc gaaaaacgac agaacgtgct
    16441 ggaagcggcg cgtattgagc tggggatgaa gcgtttcctg gaacaaggtg gcttccacgc
    16501 gttcaccacc acctttgaag atttgcacgg tctgaaacag cttccaggtc tggccgtaca
    16561 gcgtctgatg cagcagggtt acggctttgc gggcgaaggc gactggaaaa ccgccgccct
    16621 gcttcgcatc atgaaggtga tgtcaaccgg tctgcagggc ggcacctcct ttatggagga
    16681 ctacacctat cacttcgaga aaggtaatga cctggtgctc ggctcccata tgctggaagt
    16741 ctgcccgtcg attgccgtag aagagaaacc gatcctcgac gttcagcatc tcggtattgg
    16801 tggtaaggac gatcctgccc gactgatctt caatacccaa accggtccag cgattgtcgc
    16861 cagcctgatt gatctcggcg atcgttaccg tctgctggtt aactgtatcg acacggtgaa
    16921 aacaccgcac tccctgccga aactgccggt ggcgaatgcg ctgtggaaag cgcaaccgga
    16981 tctgccaact gcttccgaag cgtggatcct tgctggtggc gcgcaccata ccgtcttcag
    17041 ccatgcgctg aacctcaacg atatgcgcca gttcgccgag atgcacgaca ttgaaatcac
    17101 ggtgattgat aacgacaccc gcctgccagc gtttaaagac gcactgcgct ggaacgaagt
    17161 gtattacgga tttcgtcgct aagtagccac atcaggtatg taacgcctga tgcgacgctg
    17221 acgcgtctta tctggcctac acgctgcgat tttgtaggcc ggataagca
//
LOCUS       DAZGLJ010000099        17159 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000099 DAZGLJ010000000
VERSION     DAZGLJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.099"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            16..810
                     /gene="yjfZ"
                     /locus_tag="ACHK49_002750"
     CDS             16..810
                     /gene="yjfZ"
                     /locus_tag="ACHK49_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO9263371.1"
                     /translation="MTLPTTIYSFPAYSPHFSSTDKPVKLKFHQYARATLLPNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHKATLDNIHAQKY
                     NKAHAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNS
                     AAIYSGLGFERVPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(881..1330)
                     /gene="rplI"
                     /locus_tag="ACHK49_002751"
     CDS             complement(881..1330)
                     /gene="rplI"
                     /locus_tag="ACHK49_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO9263372.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1372..1599)
                     /gene="rpsR"
                     /locus_tag="ACHK49_002752"
     CDS             complement(1372..1599)
                     /gene="rpsR"
                     /locus_tag="ACHK49_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO9263373.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1604..1918)
                     /gene="priB"
                     /locus_tag="ACHK49_002753"
     CDS             complement(1604..1918)
                     /gene="priB"
                     /locus_tag="ACHK49_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO9263374.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1925..2320)
                     /gene="rpsF"
                     /locus_tag="ACHK49_002754"
     CDS             complement(1925..2320)
                     /gene="rpsF"
                     /locus_tag="ACHK49_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO9263375.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2647..2922
                     /gene="yjfY"
                     /locus_tag="ACHK49_002755"
     CDS             2647..2922
                     /gene="yjfY"
                     /locus_tag="ACHK49_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO9263376.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(3051..3737)
                     /gene="ulaF"
                     /locus_tag="ACHK49_002756"
     CDS             complement(3051..3737)
                     /gene="ulaF"
                     /locus_tag="ACHK49_002756"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO9263377.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(3737..4591)
                     /gene="ulaE"
                     /locus_tag="ACHK49_002757"
     CDS             complement(3737..4591)
                     /gene="ulaE"
                     /locus_tag="ACHK49_002757"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO9263378.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(4601..5251)
                     /gene="ulaD"
                     /locus_tag="ACHK49_002758"
     CDS             complement(4601..5251)
                     /gene="ulaD"
                     /locus_tag="ACHK49_002758"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO9263379.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5265..5729)
                     /gene="ulaC"
                     /locus_tag="ACHK49_002759"
     CDS             complement(5265..5729)
                     /gene="ulaC"
                     /locus_tag="ACHK49_002759"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO9263380.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(5739..6044)
                     /gene="ulaB"
                     /locus_tag="ACHK49_002760"
     CDS             complement(5739..6044)
                     /gene="ulaB"
                     /locus_tag="ACHK49_002760"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO9263381.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(6060..7457)
                     /gene="ulaA"
                     /locus_tag="ACHK49_002761"
     CDS             complement(6060..7457)
                     /gene="ulaA"
                     /locus_tag="ACHK49_002761"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO9263382.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGYIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            7812..8876
                     /gene="ulaG"
                     /locus_tag="ACHK49_002762"
     CDS             7812..8876
                     /gene="ulaG"
                     /locus_tag="ACHK49_002762"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO9263383.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            8984..9739
                     /gene="ulaR"
                     /locus_tag="ACHK49_002763"
     CDS             8984..9739
                     /gene="ulaR"
                     /locus_tag="ACHK49_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO9263384.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(9736..10485)
                     /gene="yjfP"
                     /locus_tag="ACHK49_002764"
     CDS             complement(9736..10485)
                     /gene="yjfP"
                     /locus_tag="ACHK49_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO9263385.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITTRHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            10667..10996
                     /gene="bsmA"
                     /locus_tag="ACHK49_002765"
     CDS             10667..10996
                     /gene="bsmA"
                     /locus_tag="ACHK49_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO9263386.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11145..11420
                     /gene="yjfN"
                     /locus_tag="ACHK49_002766"
     CDS             11145..11420
                     /gene="yjfN"
                     /locus_tag="ACHK49_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO9263387.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(11537..13162)
                     /gene="aidB"
                     /locus_tag="ACHK49_002767"
     CDS             complement(11537..13162)
                     /gene="aidB"
                     /locus_tag="ACHK49_002767"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO9263388.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13246..14409)
                     /gene="yjfC"
                     /locus_tag="ACHK49_002768"
     CDS             complement(13246..14409)
                     /gene="yjfC"
                     /locus_tag="ACHK49_002768"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO9263389.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(14412..15050)
                     /gene="yjfM"
                     /locus_tag="ACHK49_002769"
     CDS             complement(14412..15050)
                     /gene="yjfM"
                     /locus_tag="ACHK49_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO9263390.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(15060..15458)
                     /gene="yjfL"
                     /locus_tag="ACHK49_002770"
     CDS             complement(15060..15458)
                     /gene="yjfL"
                     /locus_tag="ACHK49_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO9263391.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(15476..16135)
                     /gene="yjfK"
                     /locus_tag="ACHK49_002771"
     CDS             complement(15476..16135)
                     /gene="yjfK"
                     /locus_tag="ACHK49_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO9263392.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16186..16884)
                     /gene="yjfJ"
                     /locus_tag="ACHK49_002772"
     CDS             complement(16186..16884)
                     /gene="yjfJ"
                     /locus_tag="ACHK49_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO9263393.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(16903..>17159)
                     /locus_tag="ACHK49_002773"
     CDS             complement(16903..>17159)
                     /locus_tag="ACHK49_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO9263394.1"
                     /translation="MIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEE
                     YYIVFGALSLKSSLEDILLEITSLVDNALDLAEITEEYSH"
BASE COUNT         4366 a         4588 c         4104 g         4101 t
ORIGIN      
        1 acaggtaaca ttacaatgac attaccaacg actatttatt catttcctgc atattcaccc
       61 catttttcgt ccaccgacaa accagtaaaa ttaaaatttc atcaatatgc acgtgctaca
      121 ttactgccaa atcgtggaag agatcataat tgcgatggca gacgtactgt tgagatccac
      181 aaactagatc tctctgactg gcaagcattc aataagttag caacgaagtg taatgcttac
      241 gatggagtaa caatgaatgg tgataattct tttggatgga accataaagc tacattagat
      301 aatattcacg cccagaaata caataaagct cacgcgggtg cgaggttaac cgcagaactg
      361 aaatatttat tgcaagatgt cgaatccttc gaacccaaca gtaaatacac aatacacgaa
      421 gtggttttgg gacctggtta tggtactcca gactataccg gccaaacgat aggatacgtt
      481 gtaacccttc ctgcccaaat gcctaattgc tggtcatctg aactaccaac aatcgatctt
      541 tatatcgacc aacttcgcac tgttacaggc gtttctaatg ccctcggatt catcattgct
      601 gcattgctaa acgcgtattc tgatttaccc catgatttga aaattggtct acgttcctta
      661 tcaaattcag cagcaatcta ctcgggttta ggtttcgagc gagtgccgca ggaccgagac
      721 ataaggagtg atcgtatgca tctaacgcca gcaaaccatc ctgacttatg gacacaagag
      781 aatggtgaat ggatctactt gcgaaactaa tacctgacga aaaatagaaa gcaaaacgcc
      841 gaccaatggt cggcgttttt acgtctcgtt gaataacgaa ttattcagct actacgttta
      901 cgatcacttt cgcgaatact tcgctgtgaa cctggaagct cacttcgtgt tcgccagtgg
      961 tacgcagaac gccgttcggc agacgaactt cgctcttagc cacttcaacg ccagctgcag
     1021 ttacagcgtc agcgatgtcg cgagtaccga tggaaccgaa cagtttacct tcgtcgccag
     1081 ctttagacgc gatggtaaca gtttccagtg cattgatttt ctcagcgcga gcattagcag
     1141 ctgccagaac ttcagccagt ttagcttcca gttcagcgcg acgtgcttcg aagaattcaa
     1201 tgtttttctt ggtagctgga acagctttac cctgcggtac caggaagtta cgagcatagc
     1261 ccgctttaac gtttacctga tcacccaggc tacccaggtt tgctacttta tcaagcagaa
     1321 taacttgcat taccttatcc tctcaaagtc gtattaatgg accgtgaccg attactgatg
     1381 gcgatcagtg tacggcagca gggacaggta gcgagcgcgt ttgatagcgc gagccagctg
     1441 acgctggtat tttgcacggg taccggtgat acggcttggg acaatcttac cgctttcggt
     1501 gatgtagttt ttcagcgtag cgatatcttt atagtcgatc tcttgaacgc cttccgcggt
     1561 gaaacggcag aacttgcgac gacggaaata acgtgccata tggctagtct ccagaatcta
     1621 tcaattcaat ctgctcggca tgcaaaacca ttttgctcag tccgttcttt gccttgtggc
     1681 atgaaatgaa cccctgaacg gttatgcgac tgccgaccgt tatactgtga gtaatggcct
     1741 ggttttcgtg tccgctaaca ataacgggca tttgacacca tgcctgccgg tgaaagccgg
     1801 cttcctcctg cacagaacga tgctcaagca cgaactggca gtgaggaatt cctgatggac
     1861 tgacctttcg aaggggagtc ctgcacacgg tgccggacaa caccagacgg ttggtcatca
     1921 gaaattactc ttcagaatcc ccagcttcag catcatcagc ggtttcgttt gcgaaatcat
     1981 cgcgacgctc acggcgctcg tctttcgctt taaccatcgg agatgcttcg gtaacagcgt
     2041 gcttggtacg cataaccatg ctgcggataa cggcatcgtt gaagcggaag gtagtttcca
     2101 gctcatcgat cacttcctgc ggagcttcaa cgttcatcag aacgtagtgt gctttgtgca
     2161 gtttgttgat cgggtaagcc agctgacggc ggccccagtc ttccagacgg tggatcttgc
     2221 cttctgcacc agtgatggca gcagtgtagc gctcgatcat gcccggaacc tgttcgctct
     2281 ggtcaggatg gaccataaaa acgatttcgt aatgacgcat cgaattgctc cttacggatt
     2341 attcagcctc ctgtctgggt cagccgaatc ccggggaggc aaggaacgtg ttaaaggtcg
     2401 gctgaaaaat gacgcgttat agtacttgta tgccccgcta aactcaaggc cagccgacaa
     2461 ataaatctca tctttcgctc aaaagaacta actccctgat ttgaagttca tcacattccg
     2521 tcacagtgtt ttttcgaaca actgcatcag aaatggtcac catcaacttc tttatcattg
     2581 attaaaatta aatcagaaga tcatcacaga ggagagcaca caaagtcagc cgttgaggag
     2641 gtatctatgt tcagtcgtgt tttagccctt ctggctgtgc ttttgctaag tgcaaataca
     2701 tgggcagcca ttgaaatcaa taaccaccag gccagaaata tggacgatgt gcaaagctta
     2761 ggcgtgattt atatcaatca taatttcgcc actgaaagtg aagcacgtca ggcattaaat
     2821 gaagaaacag atgcgcaggg cgcaacgtac taccacgtaa ttctgatgcg ggaaccgggg
     2881 agtaacggca atatgcacgc cagcgcggat atttatcgct agcaccaggt ataaccaacg
     2941 aaacattgcc atagtttgct ttgccccctt cgcagggggc tttttttgaa aaacggctgc
     3001 ggaattagac cagttatctc ccgaagaagg aaatttccgc agcgcgtgtt ttacttctgc
     3061 ccgtaataag cgttaggacc atgcttacgc atgaagtgtt tattcatcag gaagctgtcg
     3121 atgtgattga gttgtggatt aatactgcgc gcaatccacg ccattttcgc cacttcttcc
     3181 atcactaccg cgttatgcac cgcgtcgtgg gcatctttcc cccaggcgaa cggtccgtgc
     3241 tgatacacca caattcccgg cgtatgcagc ggctcggcgt tgcccagcgt ttcgataatc
     3301 actttgccag tgttcagttc atactcaccc tgcacctctt cttcgcttaa tccacgcgta
     3361 cacgggatgt caccaaagaa gtagtcagcg tgcgtggtgc ctaacgccgg gatcgccagc
     3421 cccgcctgcg cccatgcggt agcatgagtg gagtgggtat ggacaatgcc gccaagcgac
     3481 ggataacgac ggtagagttc aagatgcgtc gcggtgtcgg aagatgggcg atactcccct
     3541 tccaccacct taccgctcat atcgaccacc accatatcgt ctgctttcat ggtttcgtag
     3601 gcgacgccgc tgggcttgat caccaccagc ccgcgttcgc ggtcgatagc gctgacgttg
     3661 ccccaggtaa aggtcaccag cccgtagcgc ggcagatcca tgttggcttc aaatacctgc
     3721 tgttttagct tttgcattat gccgcctcca ccatgcccgc tttcgccatg cgcgctttca
     3781 cccaatcacg cgctttcgcc acttctgccg ccgggtcttc cgccgtttcg ctccacatct
     3841 caatcaggta cggcccgcaa tagccactct gtttgagcgt ttcgaaacaa cgttcgaaat
     3901 ccactacacc ttcgccaaac ggcacgtttt tgaagacgcc aggtttggtg tctttcacat
     3961 gtaccgcgac gatatgcccg attccggcct gcaactccat ctgcacatcg ttgtcccacg
     4021 ccgacaggtt gccgatatcc gggtagagct ggaaccacgg gttgttgaga tagtgcgcgt
     4081 aacccagcgc cttgctgatg gagttcatca acggataatc catgatctcc atcgccagcg
     4141 tcacctgcgc gcggctcgcc atctcaacgc tctctttcag gccgtcacgg aaacgacgac
     4201 gcgtttcgtt attggcttcc tgatagtaaa cgtcatagcc cgccagctgg atcacgcgaa
     4261 tgccgacgtc ctgggcgaac tggatagctt tacgcataat ctccagcccc tgcgcccgca
     4321 ccgcgtcatc ttcactgccc agcgggaaac gacgatgagc agaaaggcac atggacggca
     4381 cgcgcacgcc ggtttcaaca atcgcattca ccagcgccag acgctgctcg cggctccagt
     4441 cgaggcgcga caggcgctcg tccgtttcat ctaccgacat ctcgacaaaa tcgaagccca
     4501 acgtttttgc cagttgcagg cgttccagcc agcactcccc ggcggggagc gctttttcat
     4561 agatgccaag cgggatttgt ttggacaaca tatccgctcc ttagccccac agttcggcga
     4621 tggaacgttt gaactgacgt gcagcttcaa ccggagaagc ggcatcacgg atgctgcgac
     4681 cagcgataaa cacatgaatc ggaatacctt tgaacagcgg cagatcttcc agcgccaggc
     4741 caccggtgac ggtgactttg aagcccatat cggaaagacg tttgatcgcg gtaatgtccg
     4801 cttcgcccca cgctacgcct gcggcctgcg cgtcacggct gcggtgataa accacctgct
     4861 gaatgcctgc gtcacgccac tgctgcgcct gttcccaggt ccagtagccg gtcaattcga
     4921 tctgcacgtc gccgttaaac tcttttgcta cgtccagcgc gcctttggcg gtgttgatat
     4981 ccgcacagca aattaccgtc acccagtcag cgttggcttc gaagcacata cgcgaaagga
     5041 ttttgcccgc atcagcaatt ttagcgtctg ccagtacgat tttgtgcggg tagagcgctt
     5101 tcaggtcacg aaccgcacgc acgccttcgc ccacgcacag aatggtaccc acttcgataa
     5161 tgtcgacttc ttcggcaatc aggcgagtgg tttcgtaggc gctatccata gtctggttgt
     5221 ccagcgcgac ttgcaacatc ggtaatgaca tcttcaattc cttcttaagc tgccgcgttg
     5281 gtgcggtcaa tgagatccag tacttcctgc tcggtacggc aggcacgtaa acggtcgaaa
     5341 ttttcttcat cttcaaacag gttgacgatc tgcatgatgc ccacttcctg gtgcgtgttg
     5401 gcatcgaccg ccgccatggt gatgaggata tccaccgggt cgttatcttc gtggttgaac
     5461 tccagcggct ttttcagcgt caccagcgca aaaccggttt tcttaacgcc ctcttccggg
     5521 cgaccgtgcg gcattgccag gcctggtgcg agcacaaagt acggaccgtg ctgttcaacc
     5581 gcatccagaa tcgcctggta gtaacgcggc tcgacaacat ccgccgccac cagcaggtca
     5641 acgccgattt ttactgcgtc ctgccatgtc tcagcttcag cctgcaggcg gatggattta
     5701 ttctccgcca gcgaatcacg taatttcatg gcgcgtcctt acttcacatc ctgcgggaaa
     5761 tgcgctttga tcacttccag cagtttcggg ccaaagtcgg caggagagag catgttgcgc
     5821 acgccaacca cgtatttgtt gccggtcacg gtgatttcgc ccgcaatgtg cgtagaagcg
     5881 atgatgatat ccgcgccgct caactcgctt ttgtattcgc caaccgcgca gctatttacc
     5941 gtgtggtcaa tgtttgattg ggttaaaaac tggtccactt tcatcttcat gatcatggaa
     6001 ctgccttgtc cgttgccaca cacagccaga atacgtacgg tcataatcaa aactccttat
     6061 taagcagact gttctgccag ttgtttttct gcatcttctt ctgcgcgcag tgcgcggcca
     6121 gcgaagaaca tataagccag tgcaattaca atgatgacgg ccataaaggc gataccgatg
     6181 gagaagaagc cctgcatcat cggcggtgcc agaatcgacc agtccgccat gcccatccag
     6241 gcgctcatac cggtgagttt caccgcccag acgcagccaa agatttcaat catccccatc
     6301 accagacata tcttcagcgc cgcacgccag ccgccgaagt ggttagcgaa cacgccgatg
     6361 gtggcgttgg agaagaacat cgggataaag ccgggaataa tcaggatcga ggaaccgcag
     6421 gcgaccagga tgccaaccgc aatcagctga ccgatggtgc cccacataaa gccccaaacc
     6481 acggcgttcg gcgcgaagct atagatagcc gcacagtcaa tcgccagaac cgcaccaggg
     6541 atcaggcgct gggagatacc gttaaatgct tcagagagtt ccgccacaaa catgcgcacg
     6601 ccctgagtga tgatgaagat cgccaccgcg aaggagaaac cagtttgcag gatgtacacc
     6661 gtccagttca ctttgcctgc catcgcctgc acggtgtcga taccgaagga gagcagaatg
     6721 gcaccaaaga agatggtcat cacaatcgcc gtggagacga tgttgtcgtg gaaaatattc
     6781 agccagcctg gcagtttgag gtcttcaacg ctttcctctt ttttgccgag gaacggcgca
     6841 actttatagg caatccatga tgcaaactgt tgctggtgac cgatggagaa accgcagccg
     6901 tccgtcactt cctgagtcgg cttgtacatc atgttggagg taatgcccca gtagagcgaa
     6961 accagaatcg ccgtgcagat aatggttgtc cacatggagt agccgaaaat aaacaacgta
     7021 acggcaatca gccccgcctg ctggaacatg atataaccag tcagcatgat ggtacgaata
     7081 ccggtaatgc gacgcagcag cacgtaacag atgttcagcg ccagcgctaa caacaccgcg
     7141 taacctaccc agctataggc atcgcccatg cggtcgatgg ttgccatcat cgacgcgtaa
     7201 gtatcggaaa ttgcgccgtt aatgccgtag acttcggaca ttttcgccac caccggtttg
     7261 aaggtgctgg tgaggatgcc ggaccctgcc tgcaacaaca tgaaaccaat tatggtttta
     7321 atcgtgcctt taataataac gctgacactt ttgcgcagta ggatgtagcc cagacaggtc
     7381 acaataccca gcaacaacgg ggcattggtc atgacctggt taaaaaacac ggtaaagatg
     7441 ttgtagagga tctccataac gatctcctga agaagaggta accgggtatt ccgcacaccc
     7501 ggaatgttgt gcactcactc taattttcaa aagtaatcac aacaagatta tttatgatta
     7561 aatgtgacgc gacccgcaaa taattccaca cggatagagt gggattcaca caacgaaaca
     7621 attatttatt taaattcatt tagttaattt aactacacat tccattaaat ctttccacac
     7681 tcaaaatcaa caacaacttc cctggcaaaa agtcaaaaaa tggtcattgc cttcctgaaa
     7741 aaatagtgtc aggattaatc aaaaccaatc acatattgat tcgaattgaa ctatcatgaa
     7801 ggtaaatggc gatgagtaaa gtgaaaagta tcacccgaga atcctggatc ctgagcactt
     7861 tcccggagtg gggtagctgg ctgaatgaag aaattgaaca agagcaggtc gcccctggca
     7921 catttgcgat gtggtggcta ggctgcaccg ggatctggtt gaaatcagaa ggtggcacca
     7981 acgtttgcgt tgatttctgg tgcggcactg gcaaacaaag tcacggtaac ccgttaatga
     8041 aacagggtca ccagatgcag cgcatggctg gcgtgaaaaa actgcagcca aacctacgca
     8101 ccaccccgtt tgttcttgat ccgtttgcga ttcgccagat cgacgcggta ctggcgactc
     8161 acgatcacaa cgatcatatc gacgttaacg tcgctgctgc cgtgatgcag aactgtgctg
     8221 atgacgtacc attcattgga ccgaaaacct gcgttgattt gtggattggc tggggcgtac
     8281 cgaaagagcg ttgcatcgtg gtcaaaccgg gcgatgttgt aaaagtaaaa gacattgaaa
     8341 ttcatgcgct tgacgctttc gaccgtactg cactgatcac cctgcctgcc gatcaaaaag
     8401 cggctggcgt actgccggac ggcatggacg atcgcgcggt gaactatctg ttcaaaacgc
     8461 ctggcggctc cctgtatcac agcggcgact cccactactc taactattat gcgaagcacg
     8521 gtaacgaaca tcagatcgat gtggcgttag gatcgtatgg tgagaacccg cgcggtatta
     8581 ccgacaaaat gaccagcgcc gatatgctgc gtatgggtga agccctgaat gcgaaagtgg
     8641 ttatcccgtt ccaccacgac atctggtcaa acttccaggc cgatccgcaa gagatccgcg
     8701 tgctgtggga gatgaaaaaa gatcgtctga agtatggctt caagccgttt atctggcagg
     8761 ttggcggcaa atttacctgg ccgctggata aagacaactt cgagtaccac tatccgcgcg
     8821 gtttcgatga ttgcttcact attgaaccgg atctgccgtt caagtcattc ctgtaatctg
     8881 tcgtttatac cggatagcgt ggagaagttc gcgctatccg gcgtagtatt tcctggctat
     8941 ttcaaatatc atctaaaaaa atcaaatttt atcggaatag ctcatgactg aagcacaaag
     9001 acatcaaatc ctcctggaaa tgctcgcaca attgggcttt gtgaccgttg agaaagtcgt
     9061 tgagcgtctg ggaatttcgc ctgccactgc gcgacgcgat atcaataaac ttgacgaaag
     9121 cggcaaactg aaaaaagtgc gcaatggcgc agaagctatt acccagcagc gcccgcgctg
     9181 gacgccgatg aatctgcatc aggcgcagaa tcacgatgaa aaagtacgta tcgctaaagc
     9241 agcctcgcag ctggttaatc cgggcgaaag cgtagtcatc aactgcggct ccaccgcgtt
     9301 tctgcttggg cgggaaatgt gtggcaagcc agtgcaaatc atcactaatt atctaccgct
     9361 ggcaaattac ctgatcgatc aagaacatga cagcgtgatc attatgggtg gacagtacaa
     9421 caaaagtcag tccatcactt taagcccgca gggcagcgaa aacagtctct atgccgggca
     9481 ctggatgttt accagcggaa aagggctgac cgcagaaggg ttgtataaaa ccgatatgct
     9541 gacagcaatg gcagagcaga agatgctgag cgtggtaggg aaactggtgg tactggttga
     9601 tagcagtaag attggcgaac gcgcgggaat gctttttagc cgtgccgatc aaatcgatat
     9661 gctgatcacc ggcaaaaatg ctaacccgga aatcctgcaa caactggaag cgcaaggggt
     9721 cagcattctg cgtgtttaaa gatgctggcg gaaaaatgtc acggcagcat ctaacgcctc
     9781 aggcgtaatg cggtgacgca cgcctggctg ccatgaacag gttagcagtt tatcccgtcc
     9841 cgtctcgctt aaggcctgct gcaaacgtag tgattcgtcg gcaggcacaa cgtcatcgtc
     9901 gaggccatgc cacagcagta gaggtctgtc cccaagttgt tccaggtggt ttgtcgcttc
     9961 ccactctgcc agcggcgcga taatgttatt gaattcattc tgctgtgctg ccgtttcagg
    10021 tatcagcggt ggaaacagtg aacgggcgag cgatgtaaaa tagcccgatc ccatcatgct
    10081 ggcggtacat ttcaccgtgg ggtggcgagt ggtaattccc agtgccgtca tcgcgcccat
    10141 cgacgcgcca ccgactgcca gacggtcatc aagcagccag ttttcttcgg ctattgccgc
    10201 acgtaaagta gtgaattcct gcatactttg tagcaagatt tgccagaatt gatttaaccg
    10261 ccgcgcttcg tcgctactaa aacggctacc gtgatcgggc gcatccggca tgatcacccg
    10321 caaaccagcc tgcgccagcg caacggcaaa atagctatac accaggctgg atgaagtaaa
    10381 gccgtgataa aaaattacgc acggtaacgg ggtatctttt tgcccgacag gataagcatg
    10441 aagaacggga atatctgcca gctcgcgtga ttctatttca atcatctgtc tctccttttt
    10501 gtctggtatg acatgaagaa atccttagca caagtttctg ctggcggtat tagttcataa
    10561 tgttgagatg tgggttacgc tttcgtcacg ttttcattcg aaaatcgcgt accaggtaac
    10621 aattcgggaa cattccccca aagttaaatt aataaggcac tacactatgg ttagcaggaa
    10681 acgtaatagc gttatttacc ggtttgccag tttattattg gtgttgatgt taagtgcctg
    10741 tagcgctctg caaggtacgc cacagccagc accgccagta acgaaccatc cgcaagagat
    10801 tcgccgcgac cagacgcaag ggttacagcg aataggtagc gtaagcacga tggttcgggg
    10861 ttctccggat gacgcattag cagaaatcag agcaaaagct gtcgctgcaa aagctgatta
    10921 ttacgttgtc gtcatggtgg acgaaaccat cgtgacagga cagtggtatt cacaagccat
    10981 tttgtaccgt aaataaatca gacaacttac atagtcagct ttacattgct ttgcgtccct
    11041 atgcgtttac ctgtgcacaa taaattaccg gctggaagcc tgggcgttta ttaccgcgat
    11101 ggaatgccct gcaacatgtg gggaaacgaa aaatggagct gacgatgaaa caattacttg
    11161 cctcaccctc gctgcaatta gtgacttatc ctgcgagcgc cacggcacag tctgcagaat
    11221 tcgccagtgc tgactgcgtg acgggcctga atgaaatagg tcagatctca gttagcaata
    11281 tttcagggga tccgcaggac gtggaacgta tcgtggcgtt aaaggccgat gaacaaggtg
    11341 cgtcatggta ccgcattatt accatgtacg aagaccagca gcctgataac tggcgcgtac
    11401 aggcgatact gtatgcatga gtattatata cagcaaatga acaatgccgg atgcggcgcg
    11461 agcgccttgt ccggcctaca atgttcgcat atttcattga gttaatcgaa accagcgtcg
    11521 catcagtcgt atacgcttac acacacactc cccccgtcgc ccgcagcaat aaatcattct
    11581 ggatctgctc tgacaaccgt acgccgccgc gcgtatctaa catcacctga caccacgcct
    11641 gcgccattgg cggagaggca tatttcaaca tttgcgcacc gcagcccagc aggaatagct
    11701 gatgagtaat ctctcgcccc agttcttcag ctggcttgcg cagctgctgc tgtaaacgac
    11761 gaaccgcgcg atcaaaatag cgatcctgcc ctttcacttc cacaaacgct tccgacaata
    11821 agtcgtatac gcccgcttgc ttattgagaa cgcgcagcac atccaggcac ataatattgc
    11881 cggaaccttc ccaaatactg tttaccggca tctcccggta aagccgcggc agctcgctct
    11941 cctcgcaata accaatgccg cccagcacct ccatcgcttc ggccacaaac ggcatacctc
    12001 ttttgcagat cacaaatttt gccgcaggcg taaataaacg cgcccacagg gcttctttgg
    12061 catcggcacg ccggtcccac gctcgcgcaa gacgaaacag caacgccgtt tgcccttcaa
    12121 gctgaagtgc catgcgactt aagacatgac gcataagggg ctgttggatc aatggattac
    12181 caaaaacatg gcgttgatgt gcatgataaa tcgccagcga aaatgcacgg cgcatcatgg
    12241 catggctacc cagggcgcaa tcaaaacgcg tcatcccacc cattttcagg atcagacgaa
    12301 ttccttcccc ttcctgcccc aacaaccagc caatggcatc ctgaaactcc acttcgcagc
    12361 tggcgttaga gcgattaccc agcttatctt tcagccgctc gaggcgaatc gcgttgcgtt
    12421 gcccgtcagg caaaaagcgc ggcacaaaaa agcaggacaa accgcccgta gtctgcgcca
    12481 gcaccagatg cgcatcgctt tgcggcaccg agaaaaacca tttatgcccc accagccgat
    12541 aagagccatc ttccaggcgc tctgcgcggg tggtgttgct catgacatcg gaaccgccct
    12601 gcttttccgt cattcccatg ccaatcaaca aaccgcgttt ttgcccacct ggcaataagt
    12661 gagaatcgta gcgatcgctc agcagcggcg tggtccagtc ctgaaacggc gtgggtaaca
    12721 tctgtaacaa caatggcgtg gcggcaaagg tcatcgttat tggacataac gacccagcct
    12781 caacctgcgc atgtaacata aaacgcgccg cgcgcgccac aaatgcgccg gagcgagcgt
    12841 cttcttccca ggcaagattg tgcacccgat tggtacataa cgcctgcatc agcaggtgcc
    12901 aggcggggtg aaaacgcaca tcgtccagac ggcgtccttg cgcatcgtag cgcaataatt
    12961 caggcggatt cacattcgcc agccgcccca gttcaagaga ttcagccgtt cctaactgct
    13021 gaccaatact ggctagaaaa tcgctatccc agccagcacc ttcacgcgtt accgcttcac
    13081 agagcgcgcc atcagacagg tataagttgc tgttatttaa tggtataggt tgattaaaaa
    13141 cggtgtgagt ttgccagtgc actgtgtctc cctccatcaa tggcagtcac ccttagtatg
    13201 gtcactgcca tggattcctg acagaaagag attgctaaaa cattcttatc cagcaatgta
    13261 atgcggaacg aaacgtgagg tgtctttggt gatcagcgtg ttatcttcac ggatccccat
    13321 tccgcacgct tcatcatcga caatccagct accgatgagt gtgtagctat cgccaaatcg
    13381 cggcagaggt tgaaacgcct ggtagatcat cggttcatcg gcgtaatcac catcagcgtg
    13441 gtcaacgacg ttattctgac cgtcgaaaat ggtgacgttt ccgccttcgc gcgagtagat
    13501 cggtttacgc acatagcttt cgccagcggc aatctgcggt ttttcgccct caaaccagga
    13561 tgcaagaagg ttaggatgac cagggaagaa gcgccaaagc agcggcatta gccctttatt
    13621 actcaagata cttttccata acggctccac ccattgctcg cgacgcttgc gcagcagcgg
    13681 accgttatcg tcacgcatca tccactccag cggatacagc ttaaatgcac gctggatgac
    13741 attatcatca agatcggtca gtacaccgcc gacgcccaaa ccgagatctt caatgtagat
    13801 aaaccgcgac tcctgccctg cctgctgggc gcagtcctgc aagtacagca cggtagtcct
    13861 gtcttcatcg gtgtcctgac agcagcagaa ataaaacggc tcccggctat aaagctcgct
    13921 aaagcgtgaa atcaggcgtt cctgaatggc attgtactga tcggcatcac gcggaataat
    13981 gccgctgcgc cgggcatcct ccagccacaa ccactggaaa taagccgact catacaacga
    14041 ggtcggcgta tcggcgttgt actccagcag cttcaccggc gcattgccac accaggcaaa
    14101 atccatccgg ccatacagcg aaggatcgcg tgcgcgccag ctttcggcga tcacatccca
    14161 gtacaacggc gggatcgcca gttgcgtcag gatctcttcg tctttaaccg cgcgatcaac
    14221 cacctcaagg cacatctgat gcagttccgc agtcggtttt tcgatctgct cttcaatctg
    14281 gcgcagagta aaacggtaag cccgactctc gtcccaatag atttcattgt cgatgatatg
    14341 aaagtcgaaa ccgttgttag cggcgatctg gtccagatcc cgtcgcacag gaacgttgtg
    14401 tctcagcatg attagcctcc ccaatgccca cgggcgctgg aagaacgacc atagccgccg
    14461 cgcgaaacgg ttgacgcttt tttggtggta aagccgcctg aagagtaaga ctcttttttg
    14521 ccagaaccgg agcgccagga gtaatcaccg gaagtgttgc gccagacggg gcgggaagca
    14581 aatgaggatc cgccgctgtt atagacgaac ggctcatcgc gatctttacg aataacccgg
    14641 cttaacaaaa atccagaaac gaccgggatc caactctgtt caacattgtc gtagtagcaa
    14701 ttttcgtact tagactgaca attctgctga gtcatattct tcggaacatc ggcataaaat
    14761 gccgctttgg cgttgttcca gccacgcgcg caaatgtcgg cattattacc gtcatcaata
    14821 caatcctgca ctgtcgaata aaacgttccg tcgccgtcgt tatcgacatc gccactatcg
    14881 ccacaacctt tcaatacaaa aaaagcggca ccgcccatta gagccaacgt taagtatttt
    14941 tgtgcgtagc gattgcgacg cggttcaaaa ggattattcg gtctaccaat gcgactgatc
    15001 gctccgtgac caattttact gttatttctt gatttgcgtt ttctggccat gatcgtccat
    15061 taccatgtca tacaagctgc gttaaaaata ccgccagcca gcgcggcggt tcccataaac
    15121 attcctgctg cggtattgtg attaataatt ttttcgctta atgcgggcat ataaagtctg
    15181 acaccagcaa aaacgagtaa ctgaatcacc agtgcaatcc cgccccaggc gaaatagtct
    15241 ggaatactca ccgcattgat tgccgcactg gataatggga taacgtaacc taacaatgta
    15301 ccactgaacg ccagtgaagc cgcggtatta ttgtttttga ttaactgcca ttcgttgtgc
    15361 ggtgtaattt tagaatagat aaaaagaaaa ataatcacca tagccacgcc aataaaaaaa
    15421 taggcgctaa aggcaagaag tgaatccagt atatgcattg taaacgtcct tgtaattaac
    15481 caataatatg aagtgatgtc agcgggatat cgacacccaa cgcgcgcgaa aataaccact
    15541 ctggctcgcc gagatcgtta aaagattcct caccatttaa cagcaaatat tcgtaagtat
    15601 cttcggttac ttgccgctgg tagcccatgg taaaattatg gacctcccat ttggcatggt
    15661 tttgattttc tactttttcc agcatgtaga cgggttcgat atttcccggt tcttcgctgt
    15721 taaaaaatcg ctgccagcgt ttttcctgcc agtttaagat cattgccccc atcactttgg
    15781 cgttgatcgc ctcgcgccag tgactctctt tactgatacc gtagctctct tcatagacaa
    15841 agagcttgat atcatcaatg tcatcaatat cttcgccgcc ggtggtattg atttgcagaa
    15901 attcatcgcc tgaagtgtag tagcggaaaa tctgactacc gccgcccaga tcgatgtggc
    15961 tgacagcggc taccgtaaat tcctcacccg gcagcgcaat cagcaattca tcttccagca
    16021 aacgaaacgc taacgtgtcg agcgtaaagc cactattgag atgaagtccc agcggaccgc
    16081 gagcgatagc aggcttatta tccttgccaa acagacgctg gaaaaaacca gacatatttt
    16141 atccttaaaa tagttgccag ccttttagcg aggctggcaa aaaaattact cgccctgttg
    16201 gcgttgcagt cttgctaata catcctgggc gctactttta ttgctaccgc caattccggc
    16261 ttccgccagc ttttcatcaa gatcgcgacc gtctgcgact ttttccaact gtgcagcagc
    16321 atccaggcga gcctgacgtt cggcctgacg cgtttgcagg cgttttaagg attctgccgc
    16381 tgtcgaaacg ctggaagatg cgccaacggt agaggttgtt acagcctgtt gtgcacgctg
    16441 catggcttca gtggctttaa cgacttccat ttgctgctca aactgagcga tacgctgcgc
    16501 tgtcgctgta actgcttttt ccacgccatc acgggagact tccagattcg acaacacttg
    16561 ctcttcagcg gtaatgagat tctcaaggcg cgcgatttct tcagcaactt cgttaatcaa
    16621 cgacggatta acgttcttgc ttaacgcttc cagcgcacgg gcttccagac tggctttgcg
    16681 ctcacgtaaa tctttcagct tatcgtgact taatttcacc cgcgccagca gatcaacgcg
    16741 agattttccg gctttatcga gttcagcttt agcgtcacga atatgctgtt ccagcatgcg
    16801 cacgccctgg gtttcttcaa tggattcttc cgcctgggag ataaacgatt tccccagcgt
    16861 aaataaactt tttaaaattc ccataaatac ccgtccttgt aattagtgtg aatattcttc
    16921 tgtaatttca gccagatcca atgcgttatc taccagcgag gttatctcaa gcaggatatc
    16981 ttcaagagaa gatttaagcg ataacgcccc gaaaacaatg taatactctt cctgttgcac
    17041 gctggagatt cctaccgatg acaacggcat catcttctga tttcttaata agaaggtatt
    17101 aaattcatct ggattgctga tactacttac cggacaaata aaggtttcga taatcattt
//
LOCUS       DAZGLJ010000100        16665 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000100 DAZGLJ010000000
VERSION     DAZGLJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.100"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            7..546
                     /locus_tag="ACHK49_002774"
     CDS             7..546
                     /locus_tag="ACHK49_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO9263395.1"
                     /translation="MELLMSNVSGIGDAYYWSVFKIAEAFGLHRDTVKKRLLAANTPV
                     AATVRGNPVYALQHVGPALFSVKHEAADSVHDPSRMEPKERKDWYQSENERIKLEKEQ
                     RKLIPVDEVVIVYSSMRKAVVQVLETIPDVLERDCALTPQAVGVVQQAIDDLRYTLQE
                     KSYEACAAELIPDEEGESL"
     gene            555..2654
                     /locus_tag="ACHK49_002775"
     CDS             555..2654
                     /locus_tag="ACHK49_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO9263396.1"
                     /translation="MGFSSARNLGRDISAGFSPPRRMPISEAVKKFMRVPKGAGNSVP
                     WDPELTPYIIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWIVYTIVCDPSDMLVVQ
                     MTEDKAREHSKKRLDRTFRSSAAVKKRMSPRRNDNNVHDKTFRDGSFLKIGWPSVNIM
                     SSSDYRFVALTDYDRFPENIDSEGDGFSLASKRTTTFMSAGMTLVESSPGRDICDSKW
                     RRKSPHEAPPTTGILSLYNRGDRRRWYWSCPHCGEYFQPAMDAMTGYRNEPDPFKASE
                     AAYLLCPHCSGIITAEKKRELNSAGVWLREGQVIDRNGNVSGEPRRSRIASFWMEGPA
                     AAYQTWAQLVYKLLTAEQEYEATGSEETLRAVINTDWGLPYLPRASMEQRKSELLEQR
                     AEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRSD
                     CDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDNA
                     YKFWRRCRRDGLGKRIYLFKGDSIRRAKLISRTFPDNTGRTGRRAQAAGDVPLWLLQT
                     DALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAFD
                     LMVYAEALVILHGYEKIRWPDAPEWASRETWLECVQDSTEPSSSPEPVSTPVKKQKRK
                     KTVTDDVNPWLTSGGWL"
     gene            2651..2863
                     /locus_tag="ACHK49_002776"
     CDS             2651..2863
                     /locus_tag="ACHK49_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263397.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            2863..4371
                     /locus_tag="ACHK49_002777"
     CDS             2863..4371
                     /locus_tag="ACHK49_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9263398.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLAP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAARIAAALGMYIRKG
                     DGQSYEPDGNGRKENERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            4316..6343
                     /locus_tag="ACHK49_002778"
     CDS             4316..6343
                     /locus_tag="ACHK49_002778"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO9263399.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAQGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQSLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            6430..6753
                     /locus_tag="ACHK49_002779"
     CDS             6430..6753
                     /locus_tag="ACHK49_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFO9263400.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGITEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSEPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            6746..7021
                     /locus_tag="ACHK49_002780"
     CDS             6746..7021
                     /locus_tag="ACHK49_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO9263401.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMIVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            7033..7611
                     /locus_tag="ACHK49_002781"
     CDS             7033..7611
                     /locus_tag="ACHK49_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9263402.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTVRIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGSLTQ
                     AFEDARDHIIAAEMPKQLGYALKQQLRLWLTR"
     gene            7608..8009
                     /locus_tag="ACHK49_002782"
     CDS             7608..8009
                     /locus_tag="ACHK49_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO9263403.1"
                     /translation="MNRHTQIRQAVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDIPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8020..8763
                     /locus_tag="ACHK49_002783"
     CDS             8020..8763
                     /locus_tag="ACHK49_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9263404.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVS
                     VTGLTVSPASASVKVGATTTLTFTVKPDGASDKAISVHSSDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVTAVTVSAAG"
     gene            8824..9210
                     /locus_tag="ACHK49_002784"
     CDS             8824..9210
                     /locus_tag="ACHK49_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO9263405.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAGEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIDNAVDDEHEALTPEKS"
     gene            9219..9548
                     /locus_tag="ACHK49_002785"
     CDS             9219..9548
                     /locus_tag="ACHK49_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO9263406.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            9520..12585
                     /locus_tag="ACHK49_002786"
     CDS             9520..12585
                     /locus_tag="ACHK49_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9263407.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRLNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARVAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFSPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            12585..12914
                     /locus_tag="ACHK49_002787"
     CDS             12585..12914
                     /locus_tag="ACHK49_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9263408.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCDHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            12924..13622
                     /locus_tag="ACHK49_002788"
     CDS             12924..13622
                     /locus_tag="ACHK49_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9263409.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVIWQGRQYQVYPIQGTGFELNGKGSAARPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            13627..14370
                     /locus_tag="ACHK49_002789"
     CDS             13627..14370
                     /locus_tag="ACHK49_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO9263410.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRAPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNLEAT
                     GLYQVALSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFE"
     gene            14268..14915
                     /locus_tag="ACHK49_002790"
     CDS             14268..14915
                     /locus_tag="ACHK49_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO9263411.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGSEAIRAL
                     AMQIPAFRQKLSDGWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            14976..>16665
                     /locus_tag="ACHK49_002791"
     CDS             14976..>16665
                     /locus_tag="ACHK49_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO9263412.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQNRPSDKAWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPEGVAEYSVWGLKL"
BASE COUNT         3963 a         3932 c         5141 g         3629 t
ORIGIN      
        1 tgccagatgg agttgcttat gtcaaatgtg agcgggatcg gtgatgctta ttactggagt
       61 gtttttaaaa tcgctgaggc ctttgggctt caccgggaca cagtaaaaaa acggctcctc
      121 gcggctaaca ctcctgtggc tgcgactgtc agggggaacc ccgtttacgc cctgcagcat
      181 gtcgggccag ccctgtttag tgtgaagcat gaggcagcag actctgttca tgatccatcc
      241 cgtatggagc cgaaagagag aaaggactgg taccaatctg aaaatgaaag gatcaagctg
      301 gaaaaggagc agcgaaaact catccccgtt gatgaagtag tcatcgtcta ttcgtccatg
      361 agaaaggctg tcgtccaggt tctggagaca attccggatg ttcttgaacg cgattgcgcc
      421 ctgactcctc aggccgtcgg cgttgtacag caggccattg atgacctgcg atacactctt
      481 caggaaaaat cctacgaggc ttgtgctgct gaattaattc ctgatgagga aggagagagt
      541 ctctaggagg aataatgggt ttttcatcag cccgaaattt gggaagggac atatcggcag
      601 gattttcccc accacgtcgc atgccgattt cggaggctgt taaaaaattc atgcgtgttc
      661 ccaagggggc tggtaactcg gtgccatggg atcctgaact gacaccctac atcattgagc
      721 ccatgaactg cctggcatcg cgtgaatacg atgcggtgat ttttgttggt cctgcgcgaa
      781 cagggaagac cattggtctg atcgatggat ggattgtcta taccatcgtt tgcgatcctt
      841 cggacatgct cgttgtgcag atgaccgaag ataaggcccg cgagcattct aaaaagcgcc
      901 tcgacagaac gttcagaagc agtgcggcgg taaagaaaag aatgagtcca cgtcgtaacg
      961 acaataatgt ccatgataag acgttcaggg atggctcgtt ccttaaaatt ggttggccct
     1021 cggtcaacat tatgtcgtcg tcggattacc ggtttgtcgc cttaaccgat tacgaccgtt
     1081 ttccggagaa tatcgacagc gagggtgatg gtttctccct ggcctcaaaa cgtaccacca
     1141 catttatgtc cgctgggatg actctggtgg agagctcgcc gggacgtgac atctgcgaca
     1201 gcaaatggcg acgtaagtcg cctcatgaag cgccaccgac gactggtatt ctttcccttt
     1261 acaatcgtgg tgaccgccgc cgctggtact ggtcatgtcc gcactgcggt gaatattttc
     1321 agccagccat ggatgccatg accggctacc gtaatgaacc ggatcccttt aaagccagtg
     1381 aggcggcgta tctactttgc ccgcactgca gcggcattat cactgcggag aaaaagcgtg
     1441 agctcaatag tgcaggagtc tggttgcgtg aaggtcaggt cattgatcgt aacggcaacg
     1501 tttccggtga accgcgccgc tcccgtatcg ccagtttctg gatggaaggg ccagctgctg
     1561 cgtatcagac ctgggcgcaa ctggtttaca aattactgac tgcagaacag gagtatgaag
     1621 cgacaggaag cgaagaaaca ctcagggcgg ttatcaacac cgactgggga ttgccttatc
     1681 ttcctcgtgc cagcatggag caacgaaaaa gtgaactgct tgagcagcgg gcagagccag
     1741 ttccttcccg cagtgtgccg gatggcgtta atttccttgt ggcgacagtg gatgtgcagg
     1801 cgggacgtca tcgccgtttt gtggttcagg taacgggcta tggcagtcgt ggcgaacgct
     1861 ggattattga tcgttacaac atcacgcagt cattgcgcag tgactgcgac ggggagagcc
     1921 agcgaattga tccggccagc tatccggaag actgggatgt cctgctgacg gatgtttttc
     1981 ataaaagctg gccgctggcc tccgatcctt ctcaacaaat gcgactgatg gcaatggcgg
     2041 tggactccgg cggtgaagac ggggtcactg ataatgccta taaattctgg cgtcgttgcc
     2101 gtcgtgatgg ccttggtaaa cgtatttacc tgtttaaggg tgacagcatc cggcgcgcaa
     2161 aactgatcag ccgtacattc cctgataaca ccggacgaac gggccgccgg gcgcaggccg
     2221 caggtgatgt gccgctctgg cttcttcaga cggatgccct gaaagaccgg gtgaataacg
     2281 cgttatggcg tgactcgcca ggtccaggct atgtgcattt ccctgactgg ctggggagct
     2341 ggttttacga cgaactgacg tatgaagagc ggagcagtga cgggaaatgg agtaagccgg
     2401 gtcgcggtgc caacgaagct tttgacctga tggtgtatgc cgaggctctg gtcattctgc
     2461 atggatacga aaagatccgc tggccggatg caccggagtg ggcgagccgg gagacctggc
     2521 tggagtgtgt ccaggacagt accgaaccgt catcctcacc ggaaccggta tccacgcctg
     2581 ttaaaaaaca aaaacggaag aaaacagtaa ctgacgatgt taacccctgg ctgacttccg
     2641 gaggatggtt atgaaccaga atgatatcga agccatgatt cagcgttata cggaagctga
     2701 aatggcggtg ctggacggaa aatccgtcac ctttaatggt cagcagatga ccatggaaaa
     2761 cttatctgag atccggcagg gacggcagga gtgggagcgc cgccttgcgg ctctgattac
     2821 acgacgacgg gggcatcccg ggtaccggct ggcgaggttc tgatggcaat tcttgatgat
     2881 gtgattggcg ttttttcacc aggatggaaa gcggcaaggc tgcgttcccg tgcggtgatc
     2941 caggcttatg aggccgtaaa aacgacgcgg acacacaaag cccgacggga gaaccgaact
     3001 gccgaccagt taagccagta cggggccgtg tcgttacgtg agcaggcccg ttaccttgat
     3061 aacaaccacg atctggttat tggtgtattt gacaagctgg aagaacgggt ggtggggaaa
     3121 aacgggatta ttgtcgagcc acatccggta ttacgcaatg gggccattgc ccgtgacctg
     3181 gctgcggaga ttcgcacccg atggagtgaa tggtctgtca gcccggaagt caccgggcag
     3241 tttacccgtc cgatgctgga acgtctgatg cttcgtacct ggctgcgcga tggtgaggtg
     3301 tttgcccaga tggtttccgg gcgcataaac agcctggcgc cttctgccgg tgttcatttc
     3361 tggctggagg cgctcgagcc ggactttatt cccatgacta gtgatgagag caacaggctg
     3421 aatcagggcg tgtttgttga tgactggggg cgtcccgaaa aatatctggt gtataaaagc
     3481 cgtcccgtat ccggacggca gatggaaacc aaagaagtgg atgcagagcg aatgctgcat
     3541 cttaaatttg ttcgccgtct gcaccagatg cgcgggacgt ctttgttgtc cggtgtgctg
     3601 atccgcctca gtgccctgaa agaatatgaa gattctgagc tgactgcagc aaggatcgcc
     3661 gctgctctgg ggatgtacat ccggaaaggc gacgggcaga gctatgaacc ggatggtaat
     3721 ggcagaaagg agaatgaacg tgagcttacc attcagccgg gcattattta cgacgatctg
     3781 aaacccggcg aagaaatcgg aatggtgaag tcggatcgcc ccaatcctaa ccttgaaact
     3841 tttcgtaatg gtcagttgcg tgccgtggcg gcgggcagtc gtctgagttt ttccagtaca
     3901 gcgcgcaact ataacggcac ttacagcgcc cagcgtcagg agctggttga atccactgat
     3961 ggctacctga tcctgcagga ctggtttatt ggtgccgtca cccgcccgat gtatcgtgca
     4021 tggctgaaac aggctgtggc atccggtgtt atcaggctac cccgcgatct tgaccgttct
     4081 tcactgtata ccgcggtgta ttccggacca gtgatgccgt ggattgaccc tgttaaggag
     4141 gctgaggcct ggaaaatcca gattcgtggt ggagcggcga cagaatcaga ctgggtacgt
     4201 gctggtggtc gtaatccgga tgatgtcaaa cgtcggcgca aggccgaaat tgatgaaaac
     4261 cgcaagctgg atctggtatt tgataccgat ccggccagtg ataaaggagg cagtagtgcc
     4321 gcaacgaaac gacaggagcc gcagcacacc gacgaccagt ccgaagaata attcctggtt
     4381 caggatgcag gctggtcacc agagtgacgc ggatatttat atttatgacg agattggttt
     4441 ctggggtgtt acagcgaagc agtttatcag tgatctgaat gcactgggcg atatcaccca
     4501 cattaatctc catattaatt caccgggtgg cgatgtcttt gaaggcatcg ccatttttaa
     4561 tgcgctgaaa acacatggtg cgtccattac cgtttatgtc gacggtgtgg cggcgtcaat
     4621 ggcgtcggtc attgcgatgg taggaaaccc ggtcattatg ccggaaaact ccttcatgat
     4681 gattcataaa ccatttggct ttacgggcgg tgatgcggag gacatgcgca cctatgccga
     4741 cctgctcgat aaagttgagg cggttctgtt acccgcttat gcacagaaaa ccgggaaaac
     4801 caccgatgaa attgctgcca tgctggcgga tgagacctgg atgtccggtg ccgaatgtct
     4861 ggcacaggga tttgctgatc aggtgacacc agccgttaag gcaatggcat gtattcagtc
     4921 aaaacgtaca gaggaattta aaaagatgcc ggaatccatt cgaaatatga ttactccgcc
     4981 acgcaacagt gctccacgcg tacaggatga tggacctgca gcctcccgga cgccagtgca
     5041 ggcagcagca cccgtggtgg atgaaaacag catccgtgcg caggtcctgg cagagcaaaa
     5101 agcgcgtgta aacggtatta atgatctgtt tgccatgttt ggcgggcgtt atcagtcgtt
     5161 gcaggctcag tgtcttgccg atcctgaatg ttcgctggag caggcccgcg aaaagctgtt
     5221 gaacgagatg gggcgcgagt ccacgccatc caataaaaat accccggctc atatttatgc
     5281 cggtaacggt aattttgtgg gggacgggat ccgccaggcg ctgatggcgc gcgccgggtt
     5341 tgaaaaaact gaacgtgata atgtctacaa cgggatgacc ctgcgtgaat atgcccgtat
     5401 gtcactgact gaacgtggta ttggtgtttc cagttataac ccgatgcaga tggtcggtgc
     5461 ggcgttcaca cacagtacgt ctgacttcgg taatattctg ctggatgttg cgaacaaagc
     5521 cattctgcag ggctgggaag atgcccctga aacctatgaa cagtggacgc ggaaaggtca
     5581 gttgtctgat tttaaaattg cccatcgtgt gggtatgggg ggcttcagtg ctctgcgtca
     5641 ggtgcgtgaa ggggcagaat ataaatacgt caccaccgga gataaacagg ccactattgc
     5701 actggcgacc tatggcgagc tgttcagtat cacccgtcag gccattatca atgatgatct
     5761 gaatatgctg accgatgtcc cgatgaagct gggccgtgcg gcgaaatcca ctattgccga
     5821 tctggtttat gccattctga cgtctaaccc gaaaatctcc acagataatg taagtctgtt
     5881 cgataaagcg aaacatgcaa acgtactgga gagcgcggca atggacgtgg catcgctgga
     5941 taaagcccgc cagttgatgc gcgttcagaa agagggtgag cgtcatctga atattcgtcc
     6001 tgcgttcgta ctggtaccga cggcgatgga gtctgttgct aaccaggtca ttcgctcctc
     6061 aagtgtcaag ggggctgaca ttaacgccgg tattattaac ccggtgaaag attttgcgac
     6121 cgttattgca gagcctcgtc ttgatgataa cagccagacc accttctacc tggctgcgtc
     6181 caaaggctcc gatacgattg aagtggctta tctcaacggt gtggatacgc catatattga
     6241 tcagatggag ggcttcagtg tggatggcgt gacaacgaaa gtgcgtattg acgctggtgt
     6301 tgcgccagtt gatcaccgcg gcctggtgaa atgtacggcg taaacgtcgc agacaacaac
     6361 tctgatggcc cgtaagggct ttttttgtac ctgaaatcag cccctgaacg gggctgtgcg
     6421 gagacagtta tggcaaagaa ttttgtagaa gaaggaaaaa cggtggcgat tgttgccagt
     6481 gcagccatca gcagcggaga tctggtgcag gtgggggatg tttttgcggt ggcgctgacc
     6541 gatattccac agggagaaac aggcgacggc ataaccgaag gtgtgtttat gctgcctaag
     6601 ctgaaaacgg atgacatgaa aacgggtaag aaggtttatc tgaagtcagg aaaagttcag
     6661 ctgactaaca gcggctctga gccgctggtc ggggttgtct gggcagatgc cggaaccagt
     6721 gcagaagaag tgccggtaaa actcaatgtc tgatcccttt tcccggctgg cagcgcgtat
     6781 ggatgcgatc acggtcagaa agatgggaaa gacagcctcg attaatgatg tcgatatgat
     6841 tgtgatcccg ggagaaacac tggcagagct gaatgctctg tccggacctg cggtctctct
     6901 ggtggtgttt tcttcgggat accgcccacg gcgcggggat cgcgttgttt atgacggaca
     6961 acaatggacg gtcacacggc atgaacgttt taacggtaag ccaatgatct ttattgagta
     7021 aagaggtgtg ggatgaaggg gcttgagaat gccatccgca atctgaacag ccttgatacc
     7081 cgtatggtgc cacaggccag cgcatgggcg ataaaccgtg tggcacagaa agcggtctcg
     7141 gttgccaccc ggcaggttgc cgggaatacc gttgcgggag ataaccaggt gaaagggatc
     7201 cccctgaaac tggtacgtca gcgtgtccgg gtgtttaaag ccagtccgtc aggaaaaatg
     7261 acggtcagga tccgcgttaa ccggggcaat ctgcccgcca ttaagctggg gacagcccgg
     7321 gtcagactgg cccggcgtgg tggaaaactg cagtaccgtg gcagtgtgct gaaggtgggt
     7381 aaatatcttt tccgggatgc gtttattcag caactggcga atggtcgctg gcatgtgatg
     7441 cgacgtattg atggcaaaaa tcgttacccc attgatgtgg tgaaaatccc gctgtccgga
     7501 tcgctgacac aggcatttga agatgcccgc gaccacatca ttgctgcgga aatgccgaaa
     7561 cagctggggt atgcactaaa acaacaactg aggttatggc tgacccgatg aaccgacata
     7621 cacaaatccg ccaggccgta ctggcacgcc ttcgggaaca gtgtggagac agcgccacgt
     7681 tttttgacgg gcttccggca tttattgatg cgcaggaact gcctgccgtg gcggtgtggc
     7741 tgagtgatgc tcagtacacc ggaaaaatga cggatgaaga tgactggcag gctgttctgc
     7801 atattgccgt cttcatccgg gcacaggcac cggattcaga gctggatatg tggatggaga
     7861 gcaccatttt ccctgccctg aatgatatac cggcactttc cggactcatc gacaccctga
     7921 tcccactcgg ttttaactat caacgtgata atgagatggc cacctgggcg atggcggaaa
     7981 tcacgtacca gatcacgtac acgaattaag gaggtggtaa tgaccacacc aaatccactg
     8041 gcaaagacga aaggtgcggg gacgacgttc tggatgtata ccggcaacgg cgatgcgttt
     8101 gcgaaccctt tgtcggacac tgactggctg cgtcttgcga tggtgaagga tctgcaacct
     8161 ggcgaaatga ccgctgatgc agaagatgac acttatctcg atgatgaaga tgcagactgg
     8221 aaaacgacaa cccaggggca gaaatccgtt ggtgatactt cggcgacgct ggcctggcgt
     8281 ccgggtgaca gcgggcagaa aaaactggtt cagttgttcg actccggtga agtctgcgcg
     8341 tttcgtatca aatatcccaa cggcactgtt gatgttttcc gtggctggct gagctcactg
     8401 ggtaaaacca ttgcctcaaa agacgtgatg acccgcactg tgaaaatcag cggtgtgggg
     8461 cgtccgtatc tggcagagga aggcactgaa accgtgagcg ttaccgggct gacagtatca
     8521 ccggcgtctg ccagtgtaaa agtgggagca accaccacgc tgacctttac agtaaaacct
     8581 gacggagcca gtgacaaagc gatcagtgtg cattcgtcag atccacagac tgccacggtt
     8641 accctgaacg gacttgtggc cacggtaaaa ggcgtgaagc agggcagtgt cagcattgtg
     8701 ggcatgaccg ctgacgggaa ttttgtggct gtgactgcgg tgactgtcag cgcagcaggt
     8761 taacaggacg atactcatca tttgccccgg ttattcgggg cttttttgca ggtggagaac
     8821 atgatgtttc tgaaacagga cacgtttaat tatgaaaaac agtccgtggt gctcagtgag
     8881 ctgtccgggc tgcagagaat tgaatatctg acgtttgttc agcagcgaac ggcaaagttt
     8941 gatgccgggg agggagaact gccggaggct gaacgacaga ttgcttttct gcggatgggg
     9001 atggatatca atgcctggct ggtttcccgc tcactgtgga atgcggatca gtctaaggat
     9061 gtagagacgc tttgcgcatc cgttattaca acatggtcgt atgatgccct gggtgcgggg
     9121 gcggagatgg ttctgtcgct gagcggtatg ggggccattg ataatgccgt ggatgatgag
     9181 catgaggcgc tgacgccgga aaagtcctga cgcgggaaat gcagtttgtc atgcggcttg
     9241 cccgggagtt ccggcgggca gactggcggc ggatgctgtc ggaaatgtcg gccactgagc
     9301 ttggtgagtg gggcgattat ttccggatgc agagcttcag tgatgtgtgg atggatgcgc
     9361 agtttgcctc gctgaaggca ttgatcgtga gaatggtgtc cggcagcagt gatgctgcgg
     9421 tggctgattt cagcctttta ccggaagaga acgggatacc ggagcgaacg gacgaagaac
     9481 tgatgcatct tggggaaggt atttccggag gtgtgcgtta tggaccagat agccaacctg
     9541 gtcattgatt tggggattga tgcggcagag tttaaaaatg aaattccccg tatcaaaaac
     9601 cttctgaatg gtgcagccag cgatgcagaa cggtcttctg cccgtatgca gcgttttatg
     9661 gagcgtcaga ctcaggccgc ccggcagaca acgcaggcag cttcttcggc tgcaacagcc
     9721 gcatccgtcc atgcgcagac ggtggagaag aacgcacagg ctcatgaacg catggcccgc
     9781 gaggtggaga aaacccgcca gcgcatggag gcactgagcc agaaaatgcg cgaggaacag
     9841 gcgcaggcca tggctctggc ggaggctcag gataaagcgg ctgctgcgtt ttatcgtcag
     9901 attgacagtg tgaaacaggc cagtgcgggg ctgcaggaat tacagcgtat tcagcagcag
     9961 atccgacagg ccagaaacag tggcgggatt ggtcagcagg attatctggc gctgatttct
    10021 gaggttactg cgaaaacccg tgttcttaca caggctgagg aagaggctac ccgacagaaa
    10081 gtggcgttta tccgtcagct taaagagcag gcaacccgcc tgaatctttc atcttctgag
    10141 ttgcttcgtg ctaaggcagc ccagctgggg gtaagcagtg ctgcagaagt gtatatccgc
    10201 aaaatggagc aggcaggaaa agccacgcat tcgctgggtc tgaaaagtgc agcagcccgc
    10261 caggagatag gcgttctgat aggtgaactg gcccgcggca atttaggggc gctgagggga
    10321 tccgggataa cgctggctaa ccgtgccgga tggatagaca cactgatgtc accgaaaggc
    10381 atgatgcttg gcggggttat tggcggtatt gccgcggccg tctatggtct gggtaaagcc
    10441 tggtatgacg gtcagaagga gggggaagaa tttaaccgcc agttgtcgct gacggggcat
    10501 tatgccggag tcactgccgg gcagctgtgg acgctcagtc gtgctatttc cgggaatggt
    10561 atcacgcaac atgctgcagc cggtgcgctg gctcaggtgg tggggagtgg tgcatttcgt
    10621 ggaaacgata tcggtatggt ggcgagagtt gccgcacaga tggagcgatc ggttggccag
    10681 tcggtcagcg ataccataaa tcagtttaag cggctgaagg atgatcctgt aaatgccgcg
    10741 aaggctctgg acaatgagct gcattttctt actgccactc agcttgagca gatacgcgtc
    10801 cttggggagc aggggcggtc cagtgatgct gcacggatag ccatgtctgc actggcagag
    10861 gaaaccggtc ggcgtactgc ggatattgat aataacctca atgcgcttgg cagtacgctg
    10921 aagtatctgt ctgatttgtg gagtcgtttc tgggatgcgg ccatgaatat tggtcgtgaa
    10981 gactcgctgg atgaacagat tgccgcttta caggagaaag tgtcgcgggc gaaaagactc
    11041 ccctggacgg catcatcttc tcaggttgaa tacgatcagc agcgtcttaa cgatcttcag
    11101 gagaaaaaac gccagaagga tttgcaggat gcaaaagagc aggcagagcg gaattatcag
    11161 gagcaacaga aacgccgtaa tgctgaaaat gctgcactga accggatgaa tgaaacggaa
    11221 gcagcacgac atcagcgtga aattgcgcgt attaatgcca tgcagtacgc cgatcaggct
    11281 gtcagggatg cggcgataca acgtgaaaat gaacgttacg agaaagccct ggcatccggt
    11341 aagaaaaaaa cacgcgaaac ccgtaatgat gaggccaccc ggttattgct gcagtacagt
    11401 cagcaacagg cacaggtgga aggacagatt gctgctgcca gacagtcagc aggcattgcc
    11461 acggaaagga tgacagaagc gcataaacag cttctggctc tgcagcagcg catcagcgac
    11521 ctggacggga aaaaactgac ggcagatgaa aagagtgtgc tggcccgtaa agatgaactg
    11581 attcaggcac tgacgctgct ggatgtaaaa cagcaggagc ttcagaaaca gacggcactc
    11641 aacgagctga agaaaaaaac aattcagctg accagtcaac tggctgaaga agagcgcgct
    11701 cagcgtcagc aacatgacct ggatatcgcc acggtgggta tgggtgatca gcagcgacag
    11761 cgatatcagg tacaactgag tcttcgccag aaataccagc aacagctgga gcagttgagg
    11821 cgggatagtg agcagaaagg gacatataac acggatgact acagaaaggc cgagcaggcg
    11881 ctgacggaga gcctgaaccg acaactgaat gagaatcgcc gttactggca acagcttgaa
    11941 gttgtgcagg gtaactggaa aaacggagtc ctgcgtgcat ttcaggattt taccgtggat
    12001 gcagataata cggcagaaac agcagaacag gtgttctcgt cagccttcag caacatggga
    12061 aatggcctgg caacttttgt cactaccggc aaactcaatt tcaaatcctt cacctcttct
    12121 gtgctgtcag atatggcgaa aatcctggcg caggcaacca tgatgaaatc gataaaaggg
    12181 attggcagtg tactgggatt tgatctcagc agcctttccc tgaatgccaa tggggggatt
    12241 tatcagtctg ctgatttgag tcgttacagt ggcacggtgg ttaaccgtcc gacgtttttt
    12301 gcttttgcaa aaggcgcggg tgtgatgggg gaagcgggac ctgaagccat tctgccattg
    12361 cgtcgtggtg ctgacggtaa gctgggggtt gtggcggata ttggggggtc aggtatggcg
    12421 atgttttccc cgcagtacaa catcgagatc aataacgatg gcacgaacgg gcagataggt
    12481 ccggctgccc tgaaggcggt ttatgacctc gggaaaaaag cggcagcgga ctttatgcaa
    12541 cagcaggccc gtgatggtgg tcggttaagt ggagcatatc ggtaatggag acgtttcact
    12601 ggaaagtgcg cccggatatg aatgtggtat cagagccgaa agtggtgaca gtgaagctgg
    12661 gcgatggtta tgaacagcgt cgtgcggcgg gactgaataa ccagttgtcg acttacagcg
    12721 tgacgatacg tgttcgtaaa tgtgaccacc catctttaaa agcctttctg gaacggcacg
    12781 gtggcgtccg tgcatttcag tggacgccac cttatgactg gaaaccgatc agggtggttt
    12841 gtcgtaaatg gtcggcaagc gtgggggcgc tgtgggtaac cataacggca gattttgaac
    12901 aggtcgtggc ataggaggct ctgatgcagg atattccaca ggaaacacat catgagacga
    12961 cacgcctcac tcagtcagcc caggtggtgc tctgggaaat cgatctgaca gaggtcggtg
    13021 gtgaacgtta ttttttctgt aatgagcaga acgaaaaagg tgagccggtt atctggcagg
    13081 ggcggcagta tcaggtatac cccattcagg ggacgggatt tgaactgaac ggcaagggca
    13141 gtgctgcccg tccgacactg acggtttcta acctgtacgg tatggtcacc gggatggcgg
    13201 aagatctgca gagtctggtc ggcggaacgg tggtccggcg taaggtttac gcccgttttc
    13261 tggatgcggt gaacttcgtc aacggaaaca gtgacgccga tccggagcag gaggtgatca
    13321 gccgctggcg cattgagcag tgcagcgaac tgagcgcggt gagtgcctct tttgtactgt
    13381 ccacgccgac ggaaacggac ggcgctgttt ttccgggacg tatcatgctg gccaacacct
    13441 gcacctggac ctatcgcggt gatgagtgcg gttatcacgg tccggcagtc gcggatgaat
    13501 atgaccagcc gacgtccgat atcacgaagg ataaatgcag caaatgcctg agcggctgta
    13561 agtttcgcaa taacgtcggc aactttggcg gcttcctttc cattaacaaa ctttcgcagt
    13621 aatcccatga cagagacaga atcagcgatt ctggcgcacg cccggcgatg tgcgccagcg
    13681 gagtcgtgcg gcttcgtggt gagagcgccg gagggggaaa gatattttcc ctgcgtgaat
    13741 atttccggtg agccggagga ttatttccgg atggctccgg aggactggct gcaggcagag
    13801 atgcagggtg agattgtggc actggtccac agtcatcccg gtggtctgcc ctggctgagt
    13861 gaggctgacc ggcggctgca ggtgcagagt gatttgccgt ggtggctggt ctgccggggg
    13921 gcgattcaca agttccgctg tgtgccgcat ctcaccgggc ggcgctttga gcacggggtg
    13981 acggactgtt acacgctgtt ccgggatgct tatcatctgg cggggattga gatgccggat
    14041 tttcatcgcg gggatgactg gtggcgtaac ggtcagaatc tctatctgga taatctggag
    14101 gccacagggc tgtatcaggt ggcgttgtca tcagcacaac cgggcgatgt gctgctgtgc
    14161 tgttttggtt catcggtgcc gaatcatgcc gccatttact gcggcgacgg tgagctgctg
    14221 caccatattc ctgaacaact gagtaaacga gagaggtata ccgacaaatg gcagcgacgc
    14281 acacactccc tctggcgtca ccgggcatgg cacgcatctg cctttacggg gatttacaac
    14341 gatttggccg ccgcatcgac cttcgagtga aaacggggtc tgaagccatc cgggcgctgg
    14401 ccatgcagat cccggcgttt cgtcagaaac tgagcgacgg ctggtaccag gtacgcattg
    14461 ccgggcgtga tgcaggtgaa accgaattgt ctgcccgtct taatgagccg ctggcaaatg
    14521 gtgccgtgat ccacatcgtg ccgcgtctgg cgggagctaa aagtggcggt gtgtttcagg
    14581 tggtgctggg ggcggcgctg attgcggtgg catggtggaa ccctgtgggc tggctgggtg
    14641 ccgcggctgt atcgggcatg tatgcggcag gggccagtat gatcctgggc ggagtggcgc
    14701 agatgctggc accgaaagcc aggacgccca cggcagcaag tacagataac ggcaaacaga
    14761 acacctattt ctcctcactg gataacatgg ttgcccaggg caatgttctg cccgttctgt
    14821 acggtgaaat gcgcgtgggg tcgcgggtgg tctctcagga gatcagcacg gcagacgaag
    14881 gggatggtgg tcaggttgta gtgattggtc gctgatgaaa aacgttttat gtgaaaccgc
    14941 ctccgggcgg ttttgtcgtt tatggagcgt taggaatggg taaaggcagc agtaaggggc
    15001 atactccgcg cgaagcgaag gacaacctga agtccacgca gttgctgagt gtgatcgatg
    15061 ccatcagcga agggccgatt gaaggtccgg tggatggatt aaaaagcgtg ctgctgaaca
    15121 gtacgccagt gctggacact gaggggaata ccaacatctc cggcgtcacg gtggtgttcc
    15181 gggccggtga gcaggagcag acaccgccgg agggatttga atcctccgga tccgagacgg
    15241 tgctggggac ggaagtgaaa tacgacacgc cgatcacccg gaccatcacg tcggcaaaca
    15301 tcgaccgtct gcgctttacc ttcggtgtgc aggcactggt ggaaaccacc tcaaaggggg
    15361 acaggaatcc atcggaagtc cgcctgctgg ttcagataca acgtaacggt ggctgggtga
    15421 cggaaaaaga catcaccatt aaaggcaaaa ccacctcgca gtatctggcc tctgtggtgg
    15481 tggataacct gccgccgcgc ccgttcagta tccggatgcg caggatgacg ccggacagca
    15541 ccacagacca gctgcagaac aaaacgctct ggtcgtcata caccgaaatc atcgatgtga
    15601 aacagtgcta cccgaacacg gcactggtcg gcgtacaggt ggattcggag cagttcggca
    15661 gccagcaggt gagccgtaat tatcatctgc gcgggcgtat tctgcaggtg ccgtcgaatt
    15721 ataacccgca gacgcgacaa tacagcggta tctgggacgg aacgttaaaa ccggcataca
    15781 gcaacaacat ggcctggtgt ctgtgggata tgcttaccca tccgcgctac ggcatgggga
    15841 aacgtcttgg tgcggcggat gtggataaat gggcgctgta tgtcatcggc cagaattgcg
    15901 accagtcagt gccggacggc tttggcggca cggagccgcg catcacctgt aatgcgtacc
    15961 tgaccacaca gcgcaaggcg tgggacgttc tcagtgattt ctgctcggcg atgcgctgta
    16021 tgccggtatg gaacgggcag acgctgacgt tcgtgcagaa ccgaccgtcg gataaggcgt
    16081 ggacctataa ccgcagtaat gtggtgatgc cggatgatgg cgcgccgttc cgctacagct
    16141 tcagcgccct gaaagaccgc cataatgccg ttgaggtgaa ctggattgac ccgaacaacg
    16201 gctgggagac ggcgacagag cttgttgaag atacgcaggc cattgcccgt tacggtcgta
    16261 atgttacgaa gatggatgcc tttggctgta ccagccgggg gcaggcacac cgcgccgggc
    16321 tgtggctgat taaaacggag ctgctggaga cgcagaccgt ggatttcagc gtcggcgcag
    16381 aagggcttcg ccatgtaccg ggcgatgtta ttgaaatctg cgatgatgac tatgccggta
    16441 tcagcaccgg tggtcgtgtg ctggcggtga acagccagac ccggacgctg acgctcgacc
    16501 gtgaaatcac gctgccatcc tccggtacca cgctgataag cctggttgac ggaagtggca
    16561 atccggtcag cgtggaggtt cagtccgtca ccgacggcgt gaaggtaaaa gtgagccgtg
    16621 ttcctgaggg tgttgctgaa tacagcgtat gggggctgaa gctgc
//
LOCUS       DAZGLJ010000101        16327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000101 DAZGLJ010000000
VERSION     DAZGLJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.101"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(72..938)
                     /gene="yghU"
                     /locus_tag="ACHK49_002792"
     CDS             complement(72..938)
                     /gene="yghU"
                     /locus_tag="ACHK49_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO9263413.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            1143..3002
                     /gene="gss"
                     /locus_tag="ACHK49_002793"
     CDS             1143..3002
                     /gene="gss"
                     /locus_tag="ACHK49_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO9263414.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQEGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNINLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            3294..4793
                     /gene="pitB"
                     /locus_tag="ACHK49_002794"
     CDS             3294..4793
                     /gene="pitB"
                     /locus_tag="ACHK49_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO9263415.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKCKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVSNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGAQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(4842..5534)
                     /gene="yghT"
                     /locus_tag="ACHK49_002795"
     CDS             complement(4842..5534)
                     /gene="yghT"
                     /locus_tag="ACHK49_002795"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO9263416.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AIMDFMTARGYH"
     gene            5708..6421
                     /gene="yghS"
                     /locus_tag="ACHK49_002796"
     CDS             5708..6421
                     /gene="yghS"
                     /locus_tag="ACHK49_002796"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO9263417.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            6453..7211
                     /locus_tag="ACHK49_002797"
     CDS             6453..7211
                     /locus_tag="ACHK49_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263418.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            7257..8606
                     /locus_tag="ACHK49_002798"
     CDS             7257..8606
                     /locus_tag="ACHK49_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO9263419.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            8606..9430
                     /locus_tag="ACHK49_002799"
     CDS             8606..9430
                     /locus_tag="ACHK49_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263420.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            9442..10002
                     /locus_tag="ACHK49_002800"
     CDS             9442..10002
                     /locus_tag="ACHK49_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO9263421.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFIIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            10033..11103
                     /gene="lptF"
                     /locus_tag="ACHK49_002801"
     CDS             10033..11103
                     /gene="lptF"
                     /locus_tag="ACHK49_002801"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO9263422.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            11100..12179
                     /gene="lptG"
                     /locus_tag="ACHK49_002802"
     CDS             11100..12179
                     /gene="lptG"
                     /locus_tag="ACHK49_002802"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO9263423.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     VLVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(12215..13387)
                     /locus_tag="ACHK49_002803"
     CDS             complement(12215..13387)
                     /locus_tag="ACHK49_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO9263424.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYSSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQINAIIQAFATLKQ"
     gene            complement(13387..13635)
                     /locus_tag="ACHK49_002804"
     CDS             complement(13387..13635)
                     /locus_tag="ACHK49_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO9263425.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(13667..14581)
                     /locus_tag="ACHK49_002805"
     CDS             complement(13667..14581)
                     /locus_tag="ACHK49_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO9263426.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDSVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(14578..16266)
                     /locus_tag="ACHK49_002806"
     CDS             complement(14578..16266)
                     /locus_tag="ACHK49_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO9263427.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
BASE COUNT         3928 a         4035 c         4257 g         4107 t
ORIGIN      
        1 gtgcacgatt gccggatgcg gcgtgaacgc cttatccggc ctggatggct tgctgcgacg
       61 aacaccaacc cttacccctg acgcttatct tccgtattcg tctcgaaatc actggcgtca
      121 tggcgctcat gcaactgctc attcagcggt ccgttggtgc ggttaacaat acgcccacgt
      181 ttcaccgccg gacgttcgcc tacttctttc gcccagcgtt gtacatgctt ataactgccc
      241 gcatcaagaa actctgcggc atcatacacg ccgcctaaca ccacattgcc aaaccacggc
      301 caaatcgcca tatccgcaat ggtgtactca tcgcccgcaa taaacttatg ctgcgccagt
      361 tgcttatcca gcacgtcgag cagacgtttg gcttccatgg taaagcggtt gatggcgtac
      421 tcaattttca tcggcgcgta atggtaaaag tgaccaaaac caccgccgag gaacggtgcc
      481 gcgccctgta accagaacag ccagttcatc gtttcagtac gctttgccaa atcctgcggc
      541 aggaagtagc caaatttctc cgccagataa agcaggatcg aaccagattc aaacacgcgg
      601 atcggcggat tatgcgtatg atcgcgcagc gccgggatct tcgagtttgg gttcacttcg
      661 acaaagccgc tggagaattg atcgccatcg ccaatacgaa tcagccaggc gtcgtactct
      721 gcaccagtaa cgcccagcgc cagcagctcc tcaagcataa tcgttacttt ctgaccgttc
      781 ggcgttccca gcgaataaag ttgcaatggg tgtttgccaa cgggcagcgt tttttcatgc
      841 gtcggaccag aaaccgggcg attgatattg gcgaacgcgc cgccagcgga tttatcccac
      901 gtccagactt tcgcgggctg ataagtattg tctgtcatag tgagttgcct tctgagtggt
      961 agtgttgaag cagtgtagca ggtcacttgc tgtacagaat gtgaattttt taaccctttg
     1021 cgccagccgc tgcgatgagg tgtatgatta gtctgacctc tgtccggtta atgtcgctga
     1081 gcaggtaatc agagcacaac aattacgcca actgcttagg ttgtattgtt aaaggtaaag
     1141 tgatgagcaa aggaacgacc agccaggatg ccccgttcgg gacattattg ggctacgccc
     1201 caggtggggt agcaatctac tcttcagatt acagttctct cgatccgcag gaatacgaag
     1261 atgacgccgt attccgtagc tatatcgacg acgaatatat gggccacaag tggcaatgcg
     1321 ttgaatttgc tcgccgtttt ctctttctga attacggtgt ggtctttact gacgtgggta
     1381 tggcgtggga gattttctcg ctgcgcttcc tgcgagaagt ggttaatgac aacatcctgc
     1441 cattgcaggc atttcctaac ggctcgccgc gtgcgccggt cgcgggtgcg cttcttatct
     1501 gggataaagg cggtgaattt aaagacactg gccatgtcgc catcattacc caattgcatg
     1561 gcaacaaagt ccgtattgcg gaacagaacg tgattcattc cccgttgccg caagggcaac
     1621 agtggacgcg tgagctggag atggtggtcg aaaacggctg ctataccctg aaagacactt
     1681 ttgatgacac caccattctg ggctggatga tccagacgga agatactgaa tacagcttac
     1741 cgcagccgga aattgcaggc gagctgctga aaatcagcgg agcgcgcctg gaaaacaaag
     1801 gccagtttga cggtaaatgg ctggatgaaa aagatccgct gcaaaacgcc tatgtgcagg
     1861 ccaacggtca ggtgatcaat caggatcctt atcattacta caccattacc gagagtgccg
     1921 agcaggagct aattaaagcc accaacgagc tgcacctgat gtatcttcac gcaaccgaca
     1981 aggtgctgaa agatgacaac ctgctggcgc tgttcgacat cccgaaaatc ctctggccac
     2041 gtttgcgtct ctcctggcag cgtcgccgtc accatatgat cactggtcgt atggatttct
     2101 gcatggatga gcgtggcctg aaggtttacg aatacaacgc cgactctgcc tcatgtcata
     2161 ccgaagcggg cttgatcctc gaacgttggg cggagcaggg ctataaaggc aacggcttca
     2221 atccggcgga agggctgatt aacgaactgg ctggtgcctg gaaacacagt cgtgcacgtc
     2281 cgtttgtcca tatcatgcag gacaaagata tcgaggaaaa ctatcacgcg cagtttatgg
     2341 agcaggcgct gcaccaggag ggctttgaaa cgcgtatctt gcgcgggctg gatgaactgg
     2401 gctgggatgc tgccgggcaa ctgattgatg gggaaggacg gctggttaac tgcgtgtgga
     2461 aaacctgggc gtgggaaacc gcgtttgatc agattcgtga agttagcgac cgtgagtttg
     2521 ctgcggtgcc aatccgtacc ggtcatccgc aaaacgaagt gcgtcttatc gacgtattgc
     2581 tgcgcccgga agtgctagtc tttgagccgc tgtggacggt gatccccggc aacaaagcga
     2641 ttctgccgat cctctggtcg ctgttcccgc accatcgtta cctgctggat accgatttca
     2701 ctgttaatga tgaattggtg aaaacaggtt acgcagtgaa accgattgcc ggtcgctgtg
     2761 gtagcaatat caacctggtc agccatcatg aagaggtgct ggacaaaacc agcggtaaat
     2821 ttgccgagca gaaaaacatc tatcagcaac tgtggtgttt gccgaaagtg gacggtaaat
     2881 acattcaggt atgtaccttc accgttggcg gcaactacgg tgggacgtgt ttgcgcggtg
     2941 atgaatcact ggtcatcaaa aaagagagtg atattgaacc gttaattgtg gtgaaaaagt
     3001 aatacatgtt gtagcaaccc cgtactgatt atgaagaata attagtacgg ggattaatca
     3061 aaatattcaa tatttatata ttccaatatt tattcatttc atatgtgaat atattaacca
     3121 gtggaatacc tgtgttttga ttatttctaa aggttttgaa tgaatgctta ttgtctgata
     3181 cacaagaaat aacactcttt ttatcgttaa aaaatgatat ttcactttgc ccatgccgtt
     3241 aaagtcactg ataatgcgtc cgttcgtaaa ttcaaaatgg cgtaatctaa tatatgctaa
     3301 atttatttgt tggccttgat atatacacag ggcttttgtt attgcttgct ctggcatttg
     3361 tgttgttcta cgaagcaatc aatggttttc atgacacggc gaatgcggtg gcaaccgtta
     3421 tttatactcg tgccatgcaa ccacaacttg ctgtggtgat ggcggcattt tttaactttt
     3481 ttggcgtgtt attgggcgga cttagcgttg cctatgccat tgtccatatg ttaccaaccg
     3541 atttgttgct gaatatgggg tcaacccacg gtctggcgat ggtcttttcc atgctgctgg
     3601 cggcgattat atggaacctg gggacgtggt tcttcggttt accggcctcc agttcgcaca
     3661 ccttgattgg cgcgattatc ggcatcggtt taaccaacgc gctgttaacc ggctcatcgg
     3721 tgatggatgc gttaaacctg cgtgaagtga ccaaaatttt ctcctcgctg attgtttccc
     3781 ctatcgtcgg cctggtcatt gcgggtggtc tgattttcct gctgcgccgt ttctggagcg
     3841 ggacgaaaaa gcgtgaccgt attcaccgca ttccggaaga tcgcaaaaag aaaaaaggca
     3901 aatgtaaacc gccattctgg acgcgtattg cgctgattgt ttccgctgcg ggcgtggcgt
     3961 tttcgcacgg cgcgaacgac gggcagaaag ggatcggcct ggtgatgctg gtactggtgg
     4021 ggattgcccc tgctggcttc gtcgtcaata tgaacgcgtc cggctatgaa attacccgta
     4081 ctcgcgatgc cgtctccaac ttcgaacact atctgcaaca gcatcctgaa ctgccgcaga
     4141 agttgattgc gatggaacct ccattgcctg cggcatcgac tgatggcgcg caagtaacag
     4201 agtttcactg tcatccggca aatacctttg atgcgattgc gcgcgttaaa acgatgctgc
     4261 caggcaatat ggaaagttac gagccgttaa gcgtgagtca gcgcagccag ctgcgccgca
     4321 ttatgctgtg catctcagat acctccgcga agctggcgaa actgccaggc gtcagtaaag
     4381 aagaccagaa cctgctgaaa aaactgcgca gcgatatgtt aagcaccatt gagtacgctc
     4441 cggtgtggat catcatggcg gtagcactgg cgctcggcat tggcaccatg attggctggc
     4501 gtcgtgtagc gatgaccatc ggtgagaaga ttggtaagcg cggcatgacg tatgcgcaag
     4561 gcatggcggc acaaatgacg gcggcagtgt ctatcggtct tgccagttat attgggatgc
     4621 ccgtctccac aacacacgtc ctctcgtctg cagttgcagg gacgatggtg gtggacggcg
     4681 gtgggttaca gcgtaaaacg gtaaccagca tcctgatggc gtgggtattt actttaccgg
     4741 cggcgatttt tctttctggt gggctgtact ggatagcatt gcagttgatt taaccttcct
     4801 gaaaatgccc ggtcctggcg atcgggcatt tccatttttg gctagtgata accacgcgcg
     4861 gtcataaaat ccataatcgc tttttctgca tcaaccaaca cctgttccag tggctgattg
     4921 gcatcgatat caaccagttg tgcaccacca aaggttaact gtggcgttat ggctatcttt
     4981 cttgccagcg actccctttt gtggtcaggc ttacgcgcgc aggcaacttc aaggtcaaca
     5041 ttgagtttga tgaccagatc aggcttatgg ctcgccatcc agtgaaacgc tttacgttcc
     5101 tggcttgcca gccatgagac aaaacgacca ccttcaacgt taggtgggaa caccgtacca
     5161 tcgtaagcgc caggaatttg gtcctgagga taacggtcgg ttagaacaat taacccgcga
     5221 cgacgacagg caagcatatg acgaaagcgc agtaagcgac gggcgacaaa cgctgtaatt
     5281 accagcgccg gaactggtcc tggcaatttt tttgctgttt tcacctgatt tcgttcaatt
     5341 gttttatgta aggattttcc catcaacggt aattttgtca ctgcacgacc gacatttccg
     5401 gcttgttttc ctaaatgaac tctttcggca gcaccatatt tttcgacaac ggtaataaga
     5461 tgttcacaca ccgttgactt gcctgaacca tcgctaccaa taacggcaat taatggaggt
     5521 gttattgact gcatgaatat atccttaaat ttaaatatcc atccaaaata tttatttggt
     5581 taatatgttt ttatgaaagc gtaattcagg tcaatgtcac aattaaccat tgtcacaata
     5641 aggttgaacg gatattctga gcacggacca cgtaatattc aatacattat ttactgccgg
     5701 gtttttcgtg aatcaacgta atatgtcaat aattaattcc acgccagtgc gtgttattgc
     5761 cattgtagga tgtgatggtt caggtaaatc gaccctcacg gcaagcctgg taaatgaact
     5821 ggcagcaaga atgccaacag aacgcattta tctcgggcaa tcgtccgggc gaattggcga
     5881 atggatttca cagctccctg ttattggcgc accttttggg cgttatctgc gaagtaaagc
     5941 ggcacatgtg cacgaaaagc cctcaacacc gcctggcaat attactgcgc tggttatcta
     6001 tctgctttcc tgctggcggg cgtacaagtt tcgcaaaatg ttgtgtaaaa gccagcaagg
     6061 ctttctgctc atcaccgacc gctacccgca ggttgaagtg ccgggttttc gctttgatgg
     6121 cccgcaattg gcaaaaacca cgggcggtaa cggttggata aaaatgttaa ggcagcgcga
     6181 gctgaagctg taccaatgga tggcatctta tttgcccgta ttgttgattc gtcttggcat
     6241 tgatgaacaa accgcgtttg cgcgtaaacc tgaccaccaa ctggcagcgt tacaggaaaa
     6301 aatcgccgtt acgccgcaac tgacattcaa tggcgcaaag atccttgaac tggatgggcg
     6361 acaccccgcc gatgaaattc tgcaagcgtc actacgcgca attcacgccg cgctttcctg
     6421 atcaccaaaa tggatagggg atgatttaac gaatggatgc actacaaact caaactgtta
     6481 atagtacaac cgcgccgcag cccaactaca ttccggggct gattgcggtg gtcgggtgtg
     6541 atggcaccgg taaatccaca ctgaccaccg acctggtgaa atcgctgcaa caacactggc
     6601 aaaccgagcg gcgctatctg gggctgctct ccggcgaaga cggcgacaaa atcaaacgat
     6661 tgccgttggt tggcgtctgg ctggaacggc gactggcggc caaatcctcg aaaacccaaa
     6721 gcatgaaaac caaatctccg gcgctatggg cggcggtgat tatgtactgc ttctcgctgc
     6781 gaagaatggc gaatctacgc aaggttcagc gactggcgca aagtggcgtt ctggtggtca
     6841 gcgatcgctt cccgcaggct gaaatttcgg gcttttatta tgatggaccg gggattggcg
     6901 tcgaacgtgc gaccgggaaa atcagcatgt ttctggcgca gcgcgaacgg cgtttatacc
     6961 aacaaatggc gcaatatcgc ccggaattaa ttattcgcct gggcattgat attgagactg
     7021 ccatctcccg caagcctgac catgactatg ccgagctgca ggacaaaatc ggcgtcatgt
     7081 cgacgattgg ctataacggc acaaaaattc ttgagattga ttccagagcg ccctacagcg
     7141 aagtgctgga acaggcacag aaggcggttt ctctggttgc catcgtttct gaccgccgaa
     7201 gtttaactta aggctggcgg gatacttttt actatcaacg ataaggtcag gctgaattgg
     7261 cgggttttaa catcaaacat tggtttgcag atggcgcgtt tcgcaccatt attcgcaata
     7321 gcgcctggtt aggctccagt aatgtcgtga gcgccttgct gggtctgttg gcgctctcgt
     7381 gtgccggtaa agggatgacg cccgccatgt ttggcgtact ggtgattgtg caatcgtacg
     7441 ccaagtcgat cagcgatttt attaagtttc agacatggca actggtggtt cagtacggaa
     7501 cgccagcctt gaccaacaat aatccgcagc aatttcgcaa tgtcgtctca ttttccttct
     7561 cgctggatat cgtcagcggc gcggtggcga ttgtcggcgg cattgcctta ctgccattcc
     7621 tttcccattc attaggtctg gatgaccaaa gcttttggct ggcggcgctg tattgcacgc
     7681 tcattccttc aatggcttcc tccacgccga ccggcattct gcgtgcggta gatcgcttcg
     7741 atttaattgc tgtacagcag gcgacgaaac cttttctgcg cgcagcgggg agcgtcgtag
     7801 cctggtattt tgactttggt tttgcgggtt ttgttattgc ctggtacgtg tcgaatctgg
     7861 ttggcggcac catgtactgg tggtttgcag cgcgcgaatt acgccgcaga aatatccata
     7921 atgccttcaa attgaatctg tttgagtctg cccgacacat caaaggcgcg tggagttttg
     7981 tctggtcaac caacattgcc cactccatct ggtcggcgcg taactcgtgc agcacagtgc
     8041 tggtggggat cgtgttagga cccgctgccg ccgggttatt taaaatcgcc atgacattct
     8101 tcgacgccgc cggaacgcca gcgggtttgc tgggtaaaag tttttaccca gaggtgatgc
     8161 gtttagatcc gcgcaccacc agaccgtggt tgttaggtgt gaagtccggt ttactggcgg
     8221 gcggaatcgg tattctggtg gcgctcgcgg tgtttatcgt cggcaagccg ctcatttcgc
     8281 tggtgtttgg cgttaagtat ctcgaagcgt atgacctgat tcaggtgatg ctgggcgcaa
     8341 ttgtgatttc gatgctgggc ttccctcagg agtcattgct attgatggcg ggaaaacagc
     8401 gcgcttttct cgtggcgcaa accatcgcct caatcggcta catcgtattg ctgttcatgt
     8461 tctgtcatct gtttggtgtg ctgggagcag cgtttgctta cttcggtggg caatgtctgg
     8521 acgtggcgct ctcgttaatt ccaaccttaa aggcattttt tcagcgccat tccttgcttt
     8581 ataacgcagc cggagagaaa tcctgatgat gagaaaatat ttcccgcttg aggcgagcga
     8641 gcgtctattt gttgcgattg aagaagatga tgtcgttgat gcacaggtta gtttgccgcc
     8701 gactattgca ctaagttgta ccaccgaaat cattcatgat aattacgcgt tatgcctgca
     8761 attctggctc aatggcgttg atcgtcagga attactgcgt ttagttcgca aacaagccaa
     8821 aggcgatgag ctgactgctg atgagcgcaa acaatttaaa tacatgcgag cgcgctacaa
     8881 gcacctgcgt tttgcgcaac ggctgtattt aaagaagcat caggccggat ttttattcgg
     8941 caaaacgacc gtttttctgg ggcgttttca ggacggcttt cgtaacggta agaaaaatat
     9001 tgtgtcgtat tacggcaacc tgttacggat ttatttaagc tctccggtct ggtcgttggt
     9061 gaactattca ttacgccata gccagttaga aagcgtaagc agttttattg cctatcggca
     9121 aaagcaaatg cataccctga aagagattat tgccaaaccg cgactaaccg gcagggaatt
     9181 tcatgatgtg cgaaaaatca tcagccaaca ggtttcttat tacgacacct taagatcgct
     9241 tgatcctgaa aataaagagg cgttacagat ttcccgtttc ctcgccgcca ttaatggcct
     9301 gatgggagat aaacacgacg acatggtggc ggatgatatg gaaaaccgcc agtcgtatga
     9361 tgcaccgttg gcgctggata gcgatattcg tcagcgctta gagctgctga tttcgcgttt
     9421 tccgctatag ccggagcaat gatgtcatct cgtttgatta ttgcgttgat tatcatgctc
     9481 ttagccccgg gtgtgcaggc acacaatttc gtcactggca aaacggttac ccctgtttat
     9541 atccaggaag gcggcgagct gcttctgaat agcgacgacg aaattcacta ccaaaagtgg
     9601 aacagcacac aattagcagg aaaagtccgc attattcagt atatcgccgg gcgtaagtcg
     9661 gcgaagaaaa agaactcact gttaattaaa gcggttgaag cggcaaactt tccgcaggat
     9721 cgttttcagc ccaccaccat tgtgaacacc gatgatgcca tctttggtac tggctatttt
     9781 gttgttggca aaatcgaaaa aaataaacgc cgctatccgt gggcgcaatt cattattgac
     9841 ggcaatggac agggacgcgt tgcctggcgc ttgccggagc agagttcaac cattctggtg
     9901 ctgaataaag ccgggcaaat tcagtgggca aaggacggca gcctgacgcc ggaagaggta
     9961 gatcatgtca ttgcgctggc gcagaaactg atcaatgaat aaaaaattca aaggattgag
    10021 ggcgttagtg agatgaagct ggttgaacac tacatcatgc gtggtacgcg ccgtctggtg
    10081 ctgattatcg tcggttttct gatctttatt ttcgccagct actccgcaca gcgttatctg
    10141 accgaagcgg caaatgggac attagctctt gatgttgtgc tggatatcgt tttctacaaa
    10201 gtgctgattg cactggagat gttgttacct gttggtctgt atgtgtcagt tggcgtgacg
    10261 ctagggcaga tgtataccga ctcggaaatt accgctatct ctgcggcggg gggcagtccg
    10321 ggacgcttgt acaaagccgt tctttatctg gcgataccgc taagtatttt tgtcaccctt
    10381 ctgtcgatgt atggtcgtcc gtgggcttat acgcagattt atcaactgga gcagcagtcg
    10441 cagtcggagc tggatgttcg ccagttgcgg gcaaagaaat ttaacactaa cgataacgga
    10501 cgaatgatcc tttcgcagac ggttgatcag gataataatc gcctgactga cgcgctgatt
    10561 tatacttcta ctgccaatcg aacccgcatt ttccgcgccc gttcggttga tgtggttgac
    10621 ccatcacccg agaaaccgac cgttatgttg cacaacggca ctgcctatct tctcgatcat
    10681 caggggcgtg acgacaacga acagatctac cgtaatctgc aattacatct gaatccgctg
    10741 gatcaaagcc ctaacgtcaa acgcaaagca aaatcggtca cggagctggc gcgctccgcc
    10801 tttcctgccg atcatgccga actgcaatgg cgacaaagcc gtggcctgac agcattgttg
    10861 atggcgctgc tggccatttc attaagtcgg gtaaaaccgc ggcaagggcg attttcaaca
    10921 ttattgccac tgacgttgct gtttattgcc attttttatg gcggcgacgt ctgccgtacg
    10981 ctggtggcta acggcgcgat tcccctcatt cctggtttgt ggttagtacc cggactcatg
    11041 ctaatgggcc tgctgatgct ggtcgcacgc gacttctctt tgctgcagaa attttcccga
    11101 tgaatgtttt cagtcgctat ttaatccgtc atctctttct cggttttgcc gccgccgcag
    11161 ggctattgct gccgcttttt accaccttca acctgattaa cgaactggat gatgtcagcc
    11221 cgggcggtta tcgctggact caggcggtgc tggtggtgct aatgacctta ccgcgcacac
    11281 tggtcgaact ttcgccgttt atcgcgttat tgggtggtat tgttggactg gggcagttat
    11341 cgaaaaacag tgagcttacc gccattcgca gcacggggtt ttctatcttc cgtattgcac
    11401 tggtggtgct ggttgccggg atattgtgga ctgtttcgtt aggcgcgata gatgagtggg
    11461 tggcgtcgcc attgcagcaa caggcgttgc aaatcaaatc gaccgccacc gcgttggggg
    11521 aggacgatga cattaccggc aatatgctgt gggccaggcg tggtaatgaa tttgtgacgg
    11581 tgaaatcgct gaacgagcag ggccagccgg tgggcgtgga gatatttcat tatcgcgacg
    11641 atctctcgct tgaatcctac atttatgcac gcagtgccac cattaaagac gacaaaacgt
    11701 gggtcctgca tggtgtgaat cataaaaaat ggctgaatgg caaagaaacg ctggaaacat
    11761 tagataatct tgcctggcaa tcggccttca ccagtatgaa tcttgaagag ttatcaatgc
    11821 cggggaatac tttttctgtc cgtcagctta atcattacat ccattatttg caggaaaccg
    11881 ggcaacccag cagcgaatac cgccttgcac tgtgggaaaa actggggcaa cccatcctga
    11941 ccctggcgat gattttgctg gctgtgccgt ttacctttac tgccccgcgt tcgcctggga
    12001 tgggtagccg tctcgctgta ggcgtcatcg tgggcttact cacctggatc agctatcaaa
    12061 tcatggttaa tctgggattg ctatttgcgt taagcgcacc tgttaccgcg ctcggtttac
    12121 cggtagcgtt tgtgttggtg gcgttgagcc tggtgtattg gtatgacaga caacattaaa
    12181 cccgccgtgg cgcaaacacc acggcgggct cacattactg cttcagagta gcaaatgcct
    12241 gaatgatggc attgatttgc tcacgactgt gggcagcatt tacgctacag cgcagcagcg
    12301 tgatgcctgc aggcgcggca ggcggcagga taagattcac atacacgccc agcgagataa
    12361 gctcgcgcca gatgcgtaaa ccttcctctt tcgaaccgat tattactggc actaccgggc
    12421 taatatgtga acttaactca taacccagtt tttgcagacc gtcatacaga tggttggcgt
    12481 tatccatcag cttttgccgc agctctggtt gtgtggcaat agttcttaac gacgagcgca
    12541 cggtcgcaat gcaggacgga gaaggagagg cggtaaaaat atacgggcgg ctgctgtagc
    12601 gcagcacttc catcgcttcc gaaccgacag caaaaccgcc aatcgaggcc agacttttac
    12661 tgaaagtacc aacgataata tccacatcat cttcaacgcc gacagcttca gccagtccgc
    12721 gccccgttgc gcctaacacg ccaaacgaat gcgcttcatc gacaatcaaa taaccaccga
    12781 gacgacgttt gatatcaaca atttccgcca gcggcgcaac gtcaccgagc atgctgtaga
    12841 tgccctcaac gatgataatc gcctctttcg cccgttcacc gaggcgaacc atgcgccgtt
    12901 ccagatcttt agcatcgtta tggcggaaac gaataatttc cgcgccaccg agagaacagg
    12961 cgtcataaat actggcgtgg ctgtcggcat ccagtaacac cacggcgtta tggtcggcaa
    13021 gtgcgctgat gacgcctaaa ttagcggtat aaccggttga aaaaacgata gcggtagggc
    13081 gattgaaaaa ctcggcaatc tctttctcta acgccaggtg cggcgcataa ctgccgtttg
    13141 ccatacgaga accggtggtg ccggtgcctt gggccgctaa cgctgcctga ccttcgctga
    13201 tggcatcatg attaaaggtt aaaccgagat aattattggt cccggccaga atcacttttt
    13261 tattgccgat ccggccttcg gtggcggaat acacttcatc aatacaggtg ccaaacggtg
    13321 ttaacccgtt gtcactaaat tgcagtcgct cgccagcaag gcgtgcatat ttatcgtata
    13381 gccccattat ttattctcca gccatggaag tagagtttcc attaattgcg caggggtttt
    13441 gacatccagc agaatattaa taggaatgga aatatcgaat ctgtcttcca gcatcattaa
    13501 gagatccatc actttgatcg actccaggcc aagatcgttg acgagatcgc tgtcgggctt
    13561 aatttccaca ccgttttcga ccaaatcctg taaacaggaa aggatgtaat ccattactat
    13621 ttcacgattt accatagaaa cgttaacaca cctttaaaca agacaatcaa aatagcccgt
    13681 tgcgtaatgc gtgttccagg ctaatcccgg gaaaccaatt aatatcttca gaaatacggt
    13741 tattacttgc cgaccagtcg gcgtgggtta attcacgaat tttcgagcgg gtcagcatag
    13801 gttctttacc tgccaggcga ctcaacgcgg tactgatatc cgcaaggcag gtgagtaccg
    13861 gcagaggaat accaaccatt cgcacggaac cgcaacggac gttggcggca agttgctgta
    13921 tgcgttgcca gtcatagccg ccagcgacgc tatcgcataa ttcataggtt tgcgtctgta
    13981 cagtttcggc gcttaaccac tgacccactg cttgcgcaaa atcggtgacg tgcaggaaag
    14041 agagctgtgt atctggtgca ccaagtcgtg gcagcaggcc gcgcagcatc cagtcaaaca
    14101 gcggttttaa ctctttatcg ccgggaccat acacggctgt cgggcgaaaa acgcccagcg
    14161 taatttcgtc agccattgca gtcagccgtt gttcggcgat gtgtttggaa tttgcgtacc
    14221 aggagagctc gggatggcgc gccgccagcg aagagataaa cagaaaacgt tggcaaaagc
    14281 agctctcttt tgctgcttgc atcaggcgca ggctgccgtc aacgttacag cgggtgaaaa
    14341 tctcttcttt gtgcccgcgc acttgcccgg cgcaatggac cactgcgctg gctccggcaa
    14401 ccagcttgct aagtgaatga gtatcttcca gcgaaccgcg cacccaggtg agattatcat
    14461 tgacgtgagc gcgggcagta cgcgtcaatg cgcgaacgtg aaagccgcgg gcgagcaggt
    14521 tatcgataat atatttaccg ataaacccgg tagcgcccgt caccgcgaca gtttgattca
    14581 tgccagggat tcctgcacat taagactggc agcataagcc ttctgataac gttttttcgc
    14641 ttccgcacgg gcaggcttgc cggaggacgt tcggggaata ctgtggggcg gcaacagatc
    14701 gatagccgcg gtcacgccaa attcgctttg gatccgtgcc gccagcgcgt ggataagctg
    14761 cccgcgacgt tcttcgtcgc tgatccgaca ctggatctgc aaaatgattt tttcctgggc
    14821 ggtaacaaaa gcaatcgcat cgccagaatg aatttccggt tcttgttccg caatatattc
    14881 aatatcctgc ggccagatat tacggccacg aataataatc agatctttaa tgcgtccggt
    14941 gacatacaga taaccgtcca gcagataacc gaggtcgccg gtgtctaacc agcccgtcgc
    15001 ggcaatctcg tcttgcgaaa cctggtcgcc aaagtaaccg ctcatcaggc tggggccgga
    15061 gatgcaaata tggcctacca cacgttccgc gaccggcata cctgcttcat tgcggatttc
    15121 aatgccatgt tccggcaacg ctttgccgca gttgacgaaa gtcgatacgg cgcgtgtctc
    15181 tgcacccggc gcgacggctt taccctgata ttcgaggatg tcgcgatcca cttcgttaac
    15241 cacaaccccg gaggcttcat cagagaagct gacagccagc gcattttctg ccagtccgta
    15301 gcacggcatg aaagttttat tgtcaaagtt aacctgacgg aaacattcag cgaattgatg
    15361 gagttgttct gcggagatgg gttctgcacc aataccagcg acgcgccagc aggaaagatc
    15421 cagttcagcg agatcttttt cattcacgcg gcgctggcac aattcatagc caaacggcgg
    15481 cgcaacggaa acggtgccgc gatttttact gatcaattta agccattgca gaggacgcat
    15541 ggcaaaatcc tgagtgcgca aataatctac tgaaagctgc gtggcgacgg ggggcaggag
    15601 aaagccgacc agtcccatat catggtagaa aggcagccag gagacgcagc ggtcgccagg
    15661 gcgtaattta atgccgtcgt ggcttatagc acgtagatta gccatcactt cgcgatgggt
    15721 gataatgacg ccacggggaa aacgggtgct gccggaggtg tactggaggt aggcgatatc
    15781 gttcggaact ggacgctgga gcgcaacatc ggcttccggt aatgccttaa accaggcgtg
    15841 gcttaaaaca tgtaattcgg ggttgttatg cgtcgcggca ttgaccagtg gcaaccactc
    15901 atcaccagtg ataatggctg cgggctggca acttgccagt aaaccctgga gtttggcgct
    15961 ccaggaatcc cgctgaccga cgcccattgg aatcgccaac gggacggcga ctaagccagc
    16021 atactgacag gcaaaaaaag cctctacaaa cccgctactc gtttcggcaa tcagcgccac
    16081 gcgttcgcct tttttcaggt tcagcgataa caaccgcttc gcaccagctt cggcacgcgt
    16141 ttttaatgtc tgatattcca gttgatcttc aagttggcaa cgtctgtcat aaaaattcat
    16201 tccggcgcta ctcagagcgg cgtagtccaa agcatcaacc agcgttggaa aatcggcata
    16261 gcgcataggt agggaatgcg taaagatttt attagacata tacaccacga aaaaattgga
    16321 tatcaaa
//
LOCUS       DAZGLJ010000102        15982 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000102 DAZGLJ010000000
VERSION     DAZGLJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.102"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            106..1089
                     /locus_tag="ACHK49_002807"
     CDS             106..1089
                     /locus_tag="ACHK49_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9263428.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGNAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDSASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            1138..2622
                     /locus_tag="ACHK49_002808"
     CDS             1138..2622
                     /locus_tag="ACHK49_002808"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO9263429.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            2615..3586
                     /locus_tag="ACHK49_002809"
     CDS             2615..3586
                     /locus_tag="ACHK49_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9263430.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            3583..4539
                     /locus_tag="ACHK49_002810"
     CDS             3583..4539
                     /locus_tag="ACHK49_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9263431.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            4626..5675
                     /gene="yahK"
                     /locus_tag="ACHK49_002811"
     CDS             4626..5675
                     /gene="yahK"
                     /locus_tag="ACHK49_002811"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO9263432.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            5918..6733
                     /gene="yahL"
                     /locus_tag="ACHK49_002812"
     CDS             5918..6733
                     /gene="yahL"
                     /locus_tag="ACHK49_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO9263433.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIHP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            7145..7390
                     /gene="yahM"
                     /locus_tag="ACHK49_002813"
     CDS             7145..7390
                     /gene="yahM"
                     /locus_tag="ACHK49_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO9263434.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGQSSPMVGIRQIRLIT"
     gene            complement(7407..8078)
                     /gene="yahN"
                     /locus_tag="ACHK49_002814"
     CDS             complement(7407..8078)
                     /gene="yahN"
                     /locus_tag="ACHK49_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO9263435.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            8225..8500
                     /gene="yahO"
                     /locus_tag="ACHK49_002815"
     CDS             8225..8500
                     /gene="yahO"
                     /locus_tag="ACHK49_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO9263436.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(8598..10184)
                     /gene="prpR"
                     /locus_tag="ACHK49_002816"
     CDS             complement(8598..10184)
                     /gene="prpR"
                     /locus_tag="ACHK49_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO9263437.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIHKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRLGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            10423..11313
                     /gene="prpB"
                     /locus_tag="ACHK49_002817"
     CDS             10423..11313
                     /gene="prpB"
                     /locus_tag="ACHK49_002817"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO9263438.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTIDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEAKLDDLFARSQVK"
     gene            11358..12527
                     /gene="prpC"
                     /locus_tag="ACHK49_002818"
     CDS             11358..12527
                     /gene="prpC"
                     /locus_tag="ACHK49_002818"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO9263439.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASYPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIANRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            12561..14012
                     /gene="prpD"
                     /locus_tag="ACHK49_002819"
     CDS             12561..14012
                     /gene="prpD"
                     /locus_tag="ACHK49_002819"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO9263440.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            14052..15938
                     /gene="prpE"
                     /locus_tag="ACHK49_002820"
     CDS             14052..15938
                     /gene="prpE"
                     /locus_tag="ACHK49_002820"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO9263441.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVCGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
BASE COUNT         3846 a         3898 c         4330 g         3908 t
ORIGIN      
        1 aatagggctg tttattctgc ataaaaatat tcgaaaattc ttattctctc cagcgcgcta
       61 atttgggcga acacttcctg actaccctgc aatgaggctg aaatgatgaa taaacgtttt
      121 gttatcaata tggtgtcttc gctgttatta ggcgctgcgc taatttctgc gccgctccag
      181 gcagcggaaa aagtcgtggt gaatatctct aaagtcgatg gtatgccgtg gtttaatcgc
      241 atgggcgaag gtgtggttga ggcgggtaac gcctttggtg ttaatgcgtc acaggtgggg
      301 ccttcaagta ctgatgctcc gcaacaagtt aaaattattg aggatttaat cgcccgtaag
      361 gttaatgcaa ttactatcgt gccaaacgat gccaacgtgc tggaaccggt attcaaaaaa
      421 gcgcgagatg ccggaattgt ggttctgacc aacgaatccc ccggtcagcc aagtgctaac
      481 tgggatatcg aaatcattga taacgaaaaa tttgccgctg aatatgttga gcatatggcg
      541 aaacgcatgg ggggcaaggg cggctatgtg atttacgtgg gtagtttgac cgtgccgcag
      601 cataacttat gggctgactt gctggtgaaa taccagaaag agcattaccc ggatatgcat
      661 gaagttaccc gccgcatgcc ggttgccgag agtgtggatg actcacgtcg taccacactg
      721 gacctgatga aaacctatcc tgacttgaaa gcggttgtct cctttggttc aaacggtccg
      781 attggcgcgg gacgtgcggt gaaagagaaa cgggcgaaaa ataaagtcgc agtctatggc
      841 atgatgatcc cttcacaggc ggcatcactg attaaaagtg gtgatatcac cgaagggatt
      901 acttacgatt cggcctctgc aggttacgca ctcgcagccg tcgccagcac gttgctgaag
      961 ggtgaagaga tcaaacctgg attagaaatg cagaatctgg gtaaggccga cgtagatatg
     1021 gataagcgaa ttatccgttt ccataaggta ttactggtca acaaagacaa tatcgactca
     1081 ctttattaat ccatttcttc ggggcgaaag ccccgattct tattgccagg ggatgttatg
     1141 gaaaccttcc tttcccttcg tcatatcaac aaaacgtttc atgccacgcg agcgctgcgc
     1201 gatgtttctc tcgactttat gtcgggtgaa gtgcactgcc ttgccgggca aaacggctgt
     1261 ggtaaatcga cgttgattaa aattatgtcc ggtgtttatc gcccggatga gggagcggaa
     1321 attacgcttg gtgggaaaaa ctggtcaaag ctgacacccg ccgcttcggt ggcgcagggg
     1381 attcaggtga tttatcagga cctctcttta tttcctaacc tgagtgtctg ggagaatatc
     1441 gccgtgaatc actatcacca cggtctgttt gttaaccgcc gtcgtctgcg tgaggtggcg
     1501 caggcggcaa tgaccagtat taacgtcaca ttgccactgg atacgctcgt ttctgagctt
     1561 tcaattgccc gctgtcagct ggtagcaatt tgccgtgcac tggcgcagga cgcacggttg
     1621 atcgtgatgg atgaaccaac cgcttcgctg acccatcagg aagtgcaagg gttattacag
     1681 gtggtgcatc aattgcggga acgcgggatc tgcgtggtct tcgtcagtca tcgtctggaa
     1741 gaagtgatgg aagtttccga ccgtatttca gtgctgaaag atggtgagct ggtcgggact
     1801 tttccggctg cagagatgac cacaaaacaa ctcggtttcc tgatgaccgg ccaggagttc
     1861 gaatatcagg tgcgagagtt gtggcaggga aaatccagca ccccggtgct tgaggtgcgt
     1921 aatttaagtc gtcatgggga atatctaaat attaacttgc gggtggaagc gggcgaagtg
     1981 gtatcgattg ttggcctgct tggtgcgggg cgcacggaat tatgtctgag tctgtttggt
     2041 atgacccgac cggatgctgg cgagatcctc atcaacggcc agccggttac actgcatagt
     2101 aaccaggacg ctatccgcca cggtattggt tatgtttcgg aagatcgcat gtcgcgcggt
     2161 ttggtgatgg cgcaatctat tgaagacaac atcattagca ctgtttttca caaggtaaaa
     2221 gatcgcttcg gctttctgag tgaaactaaa gttcgtgacc tggtggacag gctaatcaag
     2281 gcattgacca tcaaagtctc tgatcctcat cttccggtga atacgctttc tggtggtaac
     2341 gcgcagcgag tatcgatcgc caaatggctg gcgattgggc cacgactgtt gattctcgat
     2401 agcccgacag tcggtgttga tatcgccaac aaagcaggaa tatacggcat tatcagcgat
     2461 ctggcggctc atggcattgc ggtattgatg atctgcgatg aaattgaaga agcgtggtat
     2521 caaagccacc ggattttggt aatgcaaaaa ggtcagatca cccatagctt cctgcctgac
     2581 agcagttctc aggccaggat tgcggaggtg gtaaatggct gaactgaaaa aacgccatga
     2641 attctggctg gcattactga ttgtggtgct gtttgttggc cttgcctggc gcagcgacga
     2701 gtttctgaca ttcggtaatt tgtacgatct cgccaataac tatgccatgt tgactattct
     2761 cgcctgtggt ttgtttgtgg tgttgatttc cggtggaatt gatatttcgt ttccagcaat
     2821 gaccatcatt gcgcaatacg gcatggtgct gttgctgcaa aaaattggtg gcaacttcgc
     2881 tgtcgcgttt gcactggcgg gctgcatcgg cattttactt ggcttaatta acgccttact
     2941 ggttaatcgc ctacgggtgc cttctatcat catcactatc tcgacgctga atattttcta
     3001 tggcctgctg ttatggttga gtaaaggtgt gtggctgtac gactttccgc cgtggtttga
     3061 gcagggggtt atgttgttca agtacaccga tgctgatggc tatgactatg gccttggtct
     3121 gccgctgatc gccatgatta cggtggtgct gctgacagcg tttatcatga atttcaccag
     3181 tgtagggcgc aaaatttatg cccttggcgg gaaccgcgaa tcagccagtc gcatcggctt
     3241 tagcgtgctg aaactgcaac ttttcgtcta cggctatatg ggattgatgt ctggcgctgc
     3301 gggtgtagtg cagtcgtgga cggtgatgac tgtcgccccc gattctcttc tgggttatga
     3361 gctgacagta ctggctgcgg tggtgcttgg cggcactagt ttgctcggcg ggcgcggcac
     3421 gttaaccggt actttgctcg gcgtggtgtt gttggcagtg atgcaaaacg ggctaaattt
     3481 attgggagtc tcgtcttact ggcaaacatt gatcaccggc atcatcatcg ttgccagcat
     3541 tagtgccacg gcgtggagtc agcatcagaa ccggagtctg ctatgaaaaa atcgtggcgt
     3601 aataacgttg agttttactt gatcggcctg ctggtgctaa ctgttgccgc gttcagcatt
     3661 actatgccgg agattttctg gtcgataagt aatttccagt ccgtcgcttc gcaaatgccc
     3721 gtgctgggca ttctggcgtt ggctatggcg gtgaccatgc tttgcggtgg cattaacttg
     3781 tcaattatag ccactgctaa cgcctgttca ctggtgatgg cgtgggtggc gactcagtat
     3841 ccaccgggta ttgctacggt tgtcgctacg ttactggcgg gcgctggcgc ggcggtgata
     3901 atcggtttat gtaacggcgt attgattgct ggtattcgag tgtcgccaat tcttgcaacg
     3961 cttgggatga tgacgttgct caaagggata aatattctgg tgacaggtgg tagcgccatt
     4021 gccaattatc cctcatgggt gctgtggttg aatcatgcgc aatggttcgg tattccgcta
     4081 ccgatgtggt tgtttgccgc agtggcactg gggctgtgga tattgctgga gaaaacgccg
     4141 ctcggtaaag ccatttacct gattgggtct aacgaacgag caacacttta ctccggcatt
     4201 aacactcgtc gggtgctgat ttgggtttat gtcatttccg ccttgctttg tgcggtagcg
     4261 gcatttttga tgatgtcgaa actcaactcg gctaaggcct cttatggcga atcgtacttg
     4321 ctggtgtcaa tcctcgcggc agtactcggc ggggttaatc cggacggcgg cagcgggcgg
     4381 ataattggta tggtgctggc gctgtttctt ctgcaaatta ttgaaagcgg cttcaatatt
     4441 ctcggtataa gcccctacct gacgatggcc ttatggggaa cgttgctgct ctgtttcatt
     4501 caggttcgcg gcatgttggg gctggatcgg gtggtttaaa tattggctat attcaatgga
     4561 cgcgttttgc cgcgatgaca tatcaggcat cgccaaatac acatagctaa tcaggagtaa
     4621 acacaatgaa gatcaaagct gttggtgcat attccgctaa acaaccactt gaaccgatgg
     4681 atatcacacg gcgtgaaccg ggaccgaatg atgtcaaaat cgaaatcgct tactgtggcg
     4741 tctgccattc cgatctccac caggtccgtt ccgagtgggc ggggacggtt tacccctgcg
     4801 taccgggtca tgaaattgtg gggcgtgtgg tagccgttgg tgatcaggta gaaaaacatg
     4861 cgccgggcga tctggtcggt gtcggctgca ttgtcgacag ttgtaaacat tgcgaagagt
     4921 gtgaagacgg gctggaaaac tactgtgatc acatgaccgg cacctataac tcgccgacgc
     4981 cggacgaacc gggccatact ctgggcggct actcacaaca gatcgtcgtt catgagcgat
     5041 atgttctgcg tattcgtcac ccgcaagagc agctggcggc ggtggctcct ttgttgtgtg
     5101 cagggatcac cacgtattcg ccgctacgtc actggcaggc cgggccgggt aaaaaagtgg
     5161 gcgtggtcgg catcggcggc ctgggacata tggggattaa gctggcccac gcgatggggg
     5221 cacatgtggt ggcatttacc acttctgagg caaaacgcga agcggcaaaa gccctggggg
     5281 ccgatgaagt tgttaactca cgcaatgccg atgagatggc ggctcatgtg aagagtttcg
     5341 atttcatttt gaatacagta gctgcgccac ataatctcga cgattttacc accttgctga
     5401 agcgtgatgg caccatgacg ctggttggtg cgcctgcgac accgcataaa tcgccggaag
     5461 ttttcaacct gatcatgaaa cgccgtgcga tagccggttc tatgattggc ggcattccag
     5521 aaactcagga gatgctcgat ttttgcgccg aacatggcat cgtggctgat atagagatga
     5581 ttcgggccga tcaaattaat gaagcctatg agcgaatgct gcgtggtgat gtgaaatatc
     5641 gttttgttat cgataatcgc acactaacag actgaaaaaa ttaataaata cgctgtggtt
     5701 taacatatta acttcgctct ccacttaact ttttagttaa ggagagcgaa ataatatcaa
     5761 agtagcagta aaacctataa cgtaaattca aattgttaaa ttaactccct ccagtacaca
     5821 atacttcaca cgttagttat gagcgatttc tgatagtgcc tggtttaatg agagctttat
     5881 tatctgcgac gtttattttt atttaagaga gatcgcgatg atatcattaa aagctccgca
     5941 caataatttg atgccatata cgcaacaaag catacttaat acggttaaaa acaatcagtt
     6001 accagaggat atcaaaagct ccctggtttc ctgtgtcgat atattcaagg ttttgattaa
     6061 acaatattat gattatcctt atgattgtcg tgatgattta gtcgatgacg acaaactcat
     6121 tcatctcatg gctgccgtac gagactgtga gtggtcagac gataacgcac tcaccataaa
     6181 tgtgcagttt aatgatttcc ctggatttta tgactggatg gattaccctg atcacccggt
     6241 taagtttgtt tttcgcatac ttgaaaatca aaaagggaca gtttgggtat atgaccagga
     6301 tgatgcgttt cttgatatta aagcaaatgt gcaggctgga cgctttaccg ggcttaagaa
     6361 attagtgcag tttatcgatt cagtgcgaac agattgcaaa tgtattcttc ttgaacacca
     6421 tatgcctttg ctgagaattt tccctaaagg gaaagagtgt atgcgtgtgg aaaaatggct
     6481 tcgcgaaatg tcgtctattc cggagacaga cgcgcccatt aagcaggcgc ttgcgcatgg
     6541 attattatta cacttaaaaa atatccaccc tgtttttcct gaatcactgg tgatgttatt
     6601 gctctctgtt ctggacgtaa aaacatacag agatgacgca cgattaaatg aatggatatc
     6661 gaatagagtg caggaattag gtgacagata ttatcctgta aataagcacg ttaaaatccg
     6721 ctacactctt tagttcagaa agggttccac ctgcaagttg ttattccacc agagagtgaa
     6781 tcgcaaagca ggtaatcatt tcatctgtat atccgtatgc caaatggcat tccaccgcct
     6841 ttttcgccga tattttgttg tgatcggcga cgcttcgctt aaaaaagcac cagcagtggt
     6901 ttcgcagcta tgcggtgtat aaaaaatgat ctcatgcaga tgttttgtga atgtgtgggt
     6961 tgacattcat atgaaaaaaa tcataattcc atcatgtttg tgtggggtct tttctgtacc
     7021 ttagcatcgc actcaagcct gacagaaaat atgctgtaag gctcatatca aaaaccgcca
     7081 ttagctcatc aggaagagca gacgacaacc aggattgttg tatggtacgg ggttcgaggc
     7141 ctcgatggcg gtccaacttt tcaaaactct tctgaatcag atccccttat tatcatccct
     7201 gcaaagtgga acattgccat tgtttggcta ttcaggacgg gtcagaccaa tgaaaaaagc
     7261 ccacacaggg gagagtgggc tgaaatggga agctaaagac tcaagtaaac ttatcggaaa
     7321 taaggaccac gcattacggg ggcaatcatc gccgatggtc gggattcggc aaatcaggtt
     7381 gattacgtag agagtaaatt atctgctcac cgctgcgtca ccccttcgta aatcaggcgt
     7441 agcgcgaata caccaataat tgcaccaata acccgactgg caacgcgttg catacgccca
     7501 taagcacgac gcacagcggg caaagaaaac gcctgactaa gaaaaactcg ccagataatt
     7561 gatgcgagca caatccccgc ccaggccatt aaacgtgccc atgttggtgt ttcggcattt
     7621 aatgttactg agaaaatact gataaaaaat aaaacggttt gcgggttaga gagatcggta
     7681 attaatccgc ggcgaaaaaa gacataccag ggggcgctaa tcggttgttg tagtgtgctc
     7741 atttgcggtg ttgactggcg gcgcatgctg caccacgcaa accataagag ataagcgccg
     7801 ccgacgattc tgataagcga aaaaatctcc tcacactgcg taattagcgt tgcaagacca
     7861 aacaaaccca acccggaata aaatgcatcg cccagcgcca cgcccagccc ggtcagcacc
     7921 cctgcgcgtc gaccggaagc caggctggtt tgtactacca caaagagatt ggctcccgga
     7981 ttaaaaaaag taatcacgaa cagtcctacg gtcaggtaaa cggcgtgcaa aggatccata
     8041 gtgatttcat ccataaataa gtgaagtaac ttcatcatat ttctaccaaa aataatcggg
     8101 tgcgagagag atcacaaagt gtcttatttc cggttactgg cgtttatgcc ctgactgaac
     8161 taattattaa tcaacccaat aatgtgggtg gatgatagtg tgataacaac tctggagccg
     8221 taatatgaaa ataatctcta aaatgttagt cggtgcgtta gcgttagccg ttaccaatgt
     8281 ctatgccgct gaattgatga ccaaagcgga atttgaaaaa gttgaatcgc agtatgaaaa
     8341 aataggtgat atttcaacta gcaatgaaat gtcgactgca gacgcgaaag aagatttgat
     8401 caaaaaagcg gatgaaaaag gggctgatgt gttggtactg acctccggtc aaactgacaa
     8461 taagatccac ggcacggcaa atatttataa gaagaagtaa ttctgaaccc tatgtaaaca
     8521 tgcccgatgc gtaagtttat cggtgatcat ctattgaaat ttatgccgga taaagcgttc
     8581 gcgctgcatt cggcagttca gcttttcagc cgccgccaga acgtcgtccg gctgatgcct
     8641 aaataattcg ccgctgctgt tttatcgcca ttaaatttct ccagtgcctg ttgtggtgtc
     8701 agtaagcgtg gagcgggagt tttcgccgac tcgcgcgcca gttccggcag tagcagctgc
     8761 aaaaattgcg gcgttaaatc cggcgtcggt tccacactta aaaatagcgc cagtcgctcc
     8821 atcatattgc gcagttcacg aatattgccc ggccagtcgt agtgcaccag cacggtttcg
     8881 cttgcctgta atcccaggcg taatgcggca gaaaacgggg cggagagcgc cgccagagac
     8941 actttcaaaa agctttccgc cagcggcaga atatccgcca cccgctcacg cagtggtggc
     9001 agttgcaggc gtaaaatgct cagccgataa aacaggtcac ggcgaaactg cccttgccgc
     9061 atatcttctt ccagcttgca gtgagtggcg ctaatgaccc gcacatccac cggaacaggc
     9121 tgatgcccgc cgacgcgggt aacctctttt tcttccagca cccgcagcag ccgggtctgc
     9181 aaaggtagcg gcatttcgcc aatctcatcc aaaaacagcg tgccaccgtg ggcgatttca
     9241 aacagcccgg cgcgacctcc gcgtcgcgag ccggtaaacg ccccttcctc atagccaaac
     9301 agctccgctt ccagcagcga ttcggcaatc gccccgcagt tgacggcaac aaacggatgc
     9361 gactttttgc cctgtcgcgc atcgtggcgg gcaaagtatt cccgatgaat tgcctgggct
     9421 gccagttctt tgcccgtccc cgtttccccc tcaatcaaca ccgccgcact ggagcgggca
     9481 tacagcaaaa tagtctgccg cacctgttcc atctgtggtg attgaccgag catatcgccc
     9541 agcacgtaac gagtacgcag ggcgttgcga gtggcatcgt gagtgttatg gcgtaacgac
     9601 atgcgcgtca tatccagcgc atcgctgaac gcctggcgca cggtggcggc ggaatagata
     9661 aaaattccgg tcattccggc ttcttctgcc agatcggtaa tcagccccgc gccgaccacc
     9721 gcttcggtgc cgttagcttt tagctcgtta atctgcccgc gtgcgtcttc ttcggtaatg
     9781 tagctacgtt gatcgaggcg caaattaaag gttttttgaa acgccaccag tgccggaata
     9841 gtttcctgat aagtgacaac gccgatagaa gaggtgagtt ttccggcttt tgccagtgcc
     9901 tgtaacacat cgtagccgct cggtttaatc aaaataactg gcactgacag gcggcttttc
     9961 aggtacgcgc cgttagatcc agccgcgatg atggcgtcac agcgttcgtt tgccagtttc
    10021 ttgtggatgt aggtcaccgc tttttcaaag ccaagctgga taggggtaat gttcgccagg
    10081 tgatcaaact cgaggctgat atcgcgaaac agctcgaaca ggcgcgttac agataccgtc
    10141 cagataaccg gtttgtcgtc attaagccgt ggtggatgtg ccatagcgca ccgcaaagtt
    10201 aagaaaccga atattgggtt tagtcttgtt tcataattgt tgcaatgaaa cgcggtgaaa
    10261 cattgcctga aacgttaact gaaacgcata tttgcggatt agttcatgac tttatctcta
    10321 acaaattgaa attaaacatt taattttatt aaggcaattg tggcacaccc cttgctttgt
    10381 ctttatcaac gcaaataaca agttgataac aaaggatggg ctatgtctct acactctcca
    10441 ggtaaagcgt ttcgcgctgc actgactaaa gaaaatccat tgcagattgt tggcaccatc
    10501 aacgctaatc atgcgctgtt ggcgcagcgt gccggatatc aggcaattta tctttctggc
    10561 ggtggcgtgg cggcaggttc gctggggctg cccgatctcg gtatttctac tcttgatgac
    10621 gtgctgaccg atattcgccg tatcaccgac gtttgttcgc tgccgctgct ggtggatgcg
    10681 gatatcggtt ttggttcttc ggcctttaac gtggcgcgca ccgtgaaatc gatgattaaa
    10741 gccggtgcgg caggattgca tattgaagat caggttggtg cgaaacgctg cggtcatcgt
    10801 ccgaataaag cgatcgtctc gaaagaagag atggtggatc ggatccgcgc ggcggtggat
    10861 gcgaaaaccg atcctgattt tgtgatcatg gcgcgcaccg atgctctggc ggtagagggg
    10921 ctggatgcgg cgatcgagcg tgcgcaggcc tatgttgaag cgggtgccga gatgttgttc
    10981 ccggaggcga ttaccgaact cgccatgtac cgccagtttg ccgatgcggt gcaggtgcca
    11041 atcctcgcca acattaccga atttggtgcc acgccgctgt ttaccatcga cgaattacgc
    11101 agcgcccatg tcgcaatggc gctgtacccg ctttcagcgt tccgcgccat gaaccgcgcc
    11161 gctgagcatg tctacaacgt cctgcgtcag gaaggcacgc agaaaagcgt catcgatacc
    11221 atgcaaaccc gcaacgagct gtacgaaagc atcaactact accagtacga agcgaagctc
    11281 gacgacctgt ttgcccgtag ccaggtgaaa taaaacacaa cgaaaaatat ctcgacccta
    11341 caaatgacga ggacaacatg agcgacacaa cgatcctgca aaacagtacc catgtcatta
    11401 aaccgaaaaa atctgtggca ctttctggcg ttccggcggg caatacggcg ctctgcaccg
    11461 tgggtaaaag tggcaatgac ctgcattacc gtggctacga tattcttgat ctggcggaac
    11521 attgcgaatt tgaagaagtg gcgcacctgc tgatccacgg caaactgccg acccgtgacg
    11581 aactcgccgc ttacaaaacg aaactgaaag ccctgcgcgg tttaccggct aacgtgcgta
    11641 ccgtgctgga agccttaccg gcggcgtcgt acccgatgga tgtcatgcgc accggcgttt
    11701 ccgcgctcgg ctgcacactg ccagaaaaag aggggcacac tgtctctggc gcgcgggata
    11761 ttgccgacaa actgctggca tcacttagct cgattctcct ttattggtat cactacagtc
    11821 acaacggcga acgcatccag ccggaaaccg atgacgactc catcggtggt cacttcctgc
    11881 atctgctgca cggcgaaaag ccatcgcaaa gctgggaaaa ggcgatgcat atttcgctag
    11941 tgctgtacgc cgaacacgag tttaacgcct ccacctttac cagtcgggtg attgcgggca
    12001 ctggctctga tatgtattcc gcgattattg gcgcgattgg cgcactgcgc gggccgaaac
    12061 acggcggagc gaatgaagtg tcgctggaga tccagcaacg ctacgaaacg ccggacgaag
    12121 ccgaagccga tatccgcaag cgggtggaaa acaaagaagt ggtcattggt tttggtcatc
    12181 cggtttatac catcgccgac ccgcgccacc aggtgatcaa acgtgtggcg aagcagctct
    12241 cgcaggaagg cggctcgttg aagatgtaca acatcgccaa tcgcctggaa acggtgatgt
    12301 gggagagcaa aaagatgttc cccaatctcg actggttctc tgctgtttcc tacaacatga
    12361 tgggcgttcc caccgagatg ttcacaccac tgtttgtcat cgcccgtgtc accggctggg
    12421 cggcgcacat cattgaacaa cgtcaggaca acaaaattat ccgtccttcc gccaattatg
    12481 ttggaccgga agaccgccag tttgtcgcgc tggataagcg ccagtaaacc tctacgaata
    12541 acaataagga aacataccca atgtcagctc aaatcaacaa catccgcccg gaatttgatc
    12601 gtgaaatcgt tgatatcgtc gattacgtga tgaactacga aatcagctct aaagtggcct
    12661 acgacaccgc acattactgc ctgctcgaca cgctcggctg cggtctggaa gctctcgaat
    12721 acccggcctg taaaaaactg ctggggccaa ttgttcctgg caccgtcgta cccaacggcg
    12781 tgcgcgtccc cggaactcag tttcagctcg accctgtcca ggcggcattt aacattggcg
    12841 cgatgatccg ctggctcgat ttcaacgata cctggctggc ggcggagtgg gggcatcctt
    12901 ccgacaacct cggcggcatt ctggcaacgg cagactggct ttcgcgcaac gctgtcgcca
    12961 gcggcaaagc gccgttgacc atgaaacagg tgctgaccgc aatgatcaaa gcccatgaaa
    13021 ttcagggctg catcgcgctg gaaaactcct ttaaccgcgt cggcctcgac cacgttctgt
    13081 tagtgaaagt ggcttccacc gccgtggtcg ccgaaatgct cggcctgacc cgcgaggaaa
    13141 ttctcaacgc cgtttcgctg gcgtgggtgg acggtcagtc gctgcgcacc tatcgccatg
    13201 cgccgaacac cggcacgcgt aaatcctggg cggcgggcga tgccacttcc cgcgcggtac
    13261 gtctggcact gatggcgaaa acgggcgaaa tgggttaccc gtcagccctg accgcgccgg
    13321 tatggggctt ctacgacgtc tcctttaaag gtgaatcgtt ccgcttccag cgtccgtacg
    13381 gttcttacgt catggagaat gtgctgttca aaatctcctt cccggcggag ttccactccc
    13441 agacggcagt tgaagcggcg atgacgctct atgaacagat gcaggcagca ggcaagacgg
    13501 cggcggatat cgaaaaagtg accattcgca cccacgaagc ctgtattcgc atcatcgaca
    13561 aaaaggggcc gctcaataac ccggctgacc gcgaccactg cattcagtac atggtggcga
    13621 tcccgctgtt attcgggcgc ttaacggcgg cagattacga ggacaacgtt gcgcaagata
    13681 aacgcatcga cgccctgcgc gagaagatca attgctttga agatccggca tttaccgctg
    13741 actaccacga cccggaaaaa cgcgccatcg ccaatgccat tacccttgag ttcaccgacg
    13801 gcacacgatt tgaagaagtg gtggtggagt accccattgg tcatgctcgc cgccgtcagg
    13861 atggtattcc gaaactggtc gataaattca aaatcaatct cgcgcgccag ttcccgactc
    13921 gccaacagca gcgcattctg gaggtttctc tcgacagaac tcgcctggaa cagatgccgg
    13981 tcaatgagta tctcgacctg tacgtcattt aagtaaactg cggtaaggcg taagttcaac
    14041 aggagagcat tatgtctttt agcgaatttt atcagcgttc gattaacgaa ccggagcagt
    14101 tctgggccga gcaggcccgg cgtattgact ggcagacgcc ctttacgcaa acgctcgacc
    14161 acagcaatcc gccgtttgcc cgttggtttt gtgaaggccg aaccaatttg tgccacaacg
    14221 ctatcgaccg ctggctggag aaacagccag aggcactggc attgattgcc gtctcttcgg
    14281 aaacagaaga agagcgcacc tttacctttc gccagctgca tgacgaagtg aacgcggtgg
    14341 cctcaatgtt gcgctcactg ggcgtgcagc gcggcgatcg ggtgctggtg tatatgccga
    14401 tgattgccga agcgcatatt actctgctgg cctgcgcgcg cattggtgct attcattcgg
    14461 tggtatttgg tggttttgcc tcgcacagcg tggcggcgcg aattgatgac gctaaaccgg
    14521 tgctgattgt ctcggctgat gccggagcgc gcggtggcaa aatcattccc tataaaaaat
    14581 tgctcgacga tgcgataagt caggcgcagc accagccacg ccatgttttg ctggtggatc
    14641 gcgggctggc gaaaatggcg cgcgtcagcg ggcgggatgt cgatttcgcg tcgttgcgcc
    14701 atcaacacat cggcgcgcgg gtaccggtgg cgtggctgga atccaacgaa acctcctgca
    14761 ttctctacac ttccggcacg accggcaaac ctaaaggcgt gcagcgtgac gtctgcggat
    14821 atgcggtggc gctggcgacc tcgatggaca ccatttttgg cggcaaagcg ggcagcgtgt
    14881 tcttttgcgc atcggatatc ggctgggtgg tggggcattc gtatatcgtt tacgcgccgc
    14941 tgctggcggg gatggcgact atcgtttacg aaggattgcc gacctggccg gactgcggcg
    15001 tgtggtggaa aatcgtcgag aaatatcagg ttagccggat gttctcagcg ccgaccgcca
    15061 ttcgcgtgct gaaaaaattc cctaccgctg aaattcgcaa acacgatctc tcgtcgctgg
    15121 aagtgctcta tctggctgga gaaccgctgg acgagccgac cgccagttgg gtgagcaata
    15181 cgctggatgt gccggtcatc gacaactact ggcagaccga atccggctgg ccgattatgg
    15241 cgattgctcg cggtctggac gacaggccga cgcgtctggg aagccccggc gtgccgatgt
    15301 atggctataa cgtgcagttg cttaatgaag tcaccggcga accgtgtggc gtcaacgaga
    15361 aagggatgct ggtggtggaa gggccgctgc cgccgggctg tattcagacc atctggggcg
    15421 acgacgaccg ctttgtgaag acttactggt cgctgttttc ccgcccggtg tacgccacct
    15481 ttgactgggg catccgtgac gctgacggtt atcactttat tctcgggcgc actgacgatg
    15541 taattaacgt tgccgggcat cgtctgggca cgcgcgagat tgaagagagt atctccagcc
    15601 atccgggcgt tgccgaagtg gcggtggttg gggtgaaaga tgcgctgaaa gggcaggtgg
    15661 cggtggcgtt tgtcattccg aaagagagcg atagtctgga agatcgtgat gtggcgcatt
    15721 cgcaagagaa ggcgattatg gcgctggtgg acagccagat tggcaacttt ggtcgcccgg
    15781 cgcacgtctg gtttgtctcg caattgccga aaacgcgatc cggaaaaatg ctgcgccgca
    15841 cgatccaggc aatttgcgaa ggacgcgatc ctggggatct gacgaccatt gatgatcctg
    15901 cgtcattgga tcagatccgc caggcgatgg aagagtaagg tattgtcgga tgcgtcgcgt
    15961 ggcgcatccg acaccatgcg cc
//
LOCUS       DAZGLJ010000103        15960 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000103 DAZGLJ010000000
VERSION     DAZGLJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.103"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..910
                     /locus_tag="ACHK49_002821"
     CDS             <1..910
                     /locus_tag="ACHK49_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9263442.1"
                     /translation="LKTLRIIICESTLISHIRYSDILNYRNELLHGETLYLDNPRSNK
                     KGRTVRTVDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALM
                     ESEKGQSQNMWKFAVYSGLRHGELAALAWEDVDLEKGIVNVRRNLTILDMFGPPKTNA
                     GIRTVTLLQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQ
                     KPYYSVSSLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQ
                     MVYEIYGMWIDDMNDEQIAMLNARLS"
     gene            962..1897
                     /gene="ttcA"
                     /locus_tag="ACHK49_002822"
     CDS             962..1897
                     /gene="ttcA"
                     /locus_tag="ACHK49_002822"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO9263443.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2026..3399)
                     /gene="dbpA"
                     /locus_tag="ACHK49_002823"
     CDS             complement(2026..3399)
                     /gene="dbpA"
                     /locus_tag="ACHK49_002823"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO9263444.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3429..3602)
                     /gene="ynaL"
                     /locus_tag="ACHK49_002824"
     CDS             complement(3429..3602)
                     /gene="ynaL"
                     /locus_tag="ACHK49_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO9263445.1"
                     /translation="MTTLIYLQIPVPEPIPSDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3884..4867)
                     /gene="zntB"
                     /locus_tag="ACHK49_002825"
     CDS             complement(3884..4867)
                     /gene="zntB"
                     /locus_tag="ACHK49_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO9263446.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4881..4928)
                     /gene="ydaN"
                     /locus_tag="ACHK49_002826"
     CDS             complement(4881..4928)
                     /gene="ydaN"
                     /locus_tag="ACHK49_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO9263447.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            5122..6354
                     /gene="dgcM"
                     /locus_tag="ACHK49_002827"
     CDS             5122..6354
                     /gene="dgcM"
                     /locus_tag="ACHK49_002827"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO9263448.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(6375..6938)
                     /gene="smrA"
                     /locus_tag="ACHK49_002828"
     CDS             complement(6375..6938)
                     /gene="smrA"
                     /locus_tag="ACHK49_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO9263449.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(7268..8176)
                     /gene="abgR"
                     /locus_tag="ACHK49_002829"
     CDS             complement(7268..8176)
                     /gene="abgR"
                     /locus_tag="ACHK49_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9263450.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            8352..9662
                     /gene="abgA"
                     /locus_tag="ACHK49_002830"
     CDS             8352..9662
                     /gene="abgA"
                     /locus_tag="ACHK49_002830"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO9263451.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQHHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9662..11107
                     /gene="abgB"
                     /locus_tag="ACHK49_002831"
     CDS             9662..11107
                     /gene="abgB"
                     /locus_tag="ACHK49_002831"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO9263452.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            11144..12670
                     /gene="abgT"
                     /locus_tag="ACHK49_002832"
     CDS             11144..12670
                     /gene="abgT"
                     /locus_tag="ACHK49_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFO9263453.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            12681..13196
                     /gene="ogt"
                     /locus_tag="ACHK49_002833"
     CDS             12681..13196
                     /gene="ogt"
                     /locus_tag="ACHK49_002833"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO9263454.1"
                     /translation="MLRLLEEKIATPLGPLWVMCDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            13391..14143
                     /gene="fnr"
                     /locus_tag="ACHK49_002834"
     CDS             13391..14143
                     /gene="fnr"
                     /locus_tag="ACHK49_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO9263455.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            14295..15245
                     /gene="uspE"
                     /locus_tag="ACHK49_002835"
     CDS             14295..15245
                     /gene="uspE"
                     /locus_tag="ACHK49_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO9263456.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(15295..15552)
                     /gene="ynaJ"
                     /locus_tag="ACHK49_002836"
     CDS             complement(15295..15552)
                     /gene="ynaJ"
                     /locus_tag="ACHK49_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO9263457.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            15796..>15960
                     /locus_tag="ACHK49_002837"
     CDS             15796..>15960
                     /locus_tag="ACHK49_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFO9263458.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIIS"
BASE COUNT         4104 a         3966 c         3913 g         3977 t
ORIGIN      
        1 attaaaaaca ctcagaataa taatttgtga aagtaccctg atatcacata ttcgttatag
       61 cgatatctta aactaccgga atgaactgct gcatggagaa acgctttacc tggataatcc
      121 aagatccaac aaaaaaggaa gaaccgtgcg cacagttgat aactatatcg ccctgctctg
      181 ttcgctgtta cgttttgcgt atcagtcggg atttatatca accaaaccat ttgaaggagt
      241 aaaaaaatta cagcgaaaca gaataaagcc tgatccgtta tctaaaacag aattcaatgc
      301 attaatggaa agtgaaaaag gacagagcca gaacatgtgg aaatttgcag tttactcagg
      361 acttcgtcac ggggaactgg cagctctggc gtgggaggat gtggatctcg aaaagggaat
      421 agtgaatgtc agaagaaacc tgacgatact tgatatgttc ggtcccccaa aaacaaatgc
      481 cgggatccgg acagtaacac tactgcagcc tgctcttgaa gcactgaagg agcaatacaa
      541 actgaccggg catcatcgca aaagcgaaat caccttttat catcgggagt acggcagaac
      601 cgaaaagcaa aaactgcatt ttgttttcat gcccagggtg tgtaacggaa aacaaaaacc
      661 ttattactcg gtaagcagtt tgggggcaag gtggaatgca gcagtaaaac gtgctggtat
      721 tcgccgccgt aatccgtacc atacgcggca tacttttgcc tgctggctgt tgacggcagg
      781 agcgaacccg gcatttatag ccagccaaat ggggcatgaa actgcgcaga tggtgtatga
      841 aatttacggt atgtggattg atgacatgaa cgacgaacag atagccatgt tgaatgcgcg
      901 gttatcatag ttgcaaagtt tgcccccaat ttgccccatt tagtaccaga gaactgaaat
      961 aatgcaagaa aatcaacaaa ttacaaagaa agaacaatac aacctgaaca aattacaaaa
     1021 acgtctgcgt cgtaacgtgg gcgaagccat tgctgacttc aatatgattg aagaaggcga
     1081 tcgcatcatg gtttgcctct ccgggggtaa agacagctat accatgctgg agattctgcg
     1141 caatttgcag caaagcgcgc caatcaattt ttcgctggtg gctgttaacc tcgatcaaaa
     1201 gcaaccgggc ttcccggaac acgttctgcc cgagtatctt gaaaagctgg gcgttgagta
     1261 caagattgtt gaagagaata cttacggtat cgtgaaagag aagattccag agggcaaaac
     1321 cacttgctca ctgtgttctc gccttcgtcg cggtatcctt tatcgtaccg caacggaact
     1381 gggggcgacg aagatcgcgt tgggtcacca tcgtgacgat atcctgcaaa cgttgttctt
     1441 aaatatgttc tacggcggca agatgaaagg tatgcctccg aaactgatga gcgatgatgg
     1501 caaacatatc gttattcgtc cgctggccta ctgccgcgag aaagatattc agcgatttgc
     1561 cgatgcaaaa gcgttcccga ttattccgtg caacctgtgc ggttcacagc ctaacctgca
     1621 acgtcaggtg attgctgaca tgttgcgtga ctgggataaa cgttatccag ggcgtatcga
     1681 gacgatgttc agcgcgatgc agaatgtggt gccgtcgcat ctgtgcgata ccaacctgtt
     1741 cgattttaaa ggcattaccc acggttctga agtggttaac gggggtgatc tggcgtttga
     1801 tcgcgaagag atcccactac aaccggcggg ctggcagcca gaagaagatg aaaatcagtt
     1861 ggatgagtta cgactgaatg tggttgaagt gaaataacca ggatagcgcc cgatgcgcaa
     1921 gcgtatcggg ctactcttat ggaggccgga taagacgcgg ccagcgtcgc atccggcaat
     1981 cccgaataag atgtttactc ttgcacccgg caattcaaca tttcattatt ttaataaccg
     2041 cacccggcac gtttttcctt taatcttccc gccctgtaac tgtttccatg ctttatgagc
     2101 aacagcctga cggaccgcga catagacatg cgccggatgc acggcgattt tgccaatatc
     2161 tgcgccatca agcccgatat ctcctgtcag tgcacctaat acatcacccg ggcgcatttt
     2221 ggcttttttc ccgccatcga tacacaacgt tgccatttct gcttccagcg gcacaatgga
     2281 actattagct ggcggcgttt gccagttaag ttttatctgc aacatgtcag aaatgatatt
     2341 ggcccgctgt gcttcttccg gagcacagaa actgatcgcc agaccgctat ttcctgcacg
     2401 agctgtacga ccgatgcgat gtacatgaac ttcagggtcc cacgccagct caaagttcac
     2461 caccagctca agcgatttaa tatccagacc acgcgcagca acatcagtag caaccagtac
     2521 acgggcgcta ccgttagcaa aacgtaccag agtctgatcg cgatcgcgtt gctccagatc
     2581 gccgtgtaac gacaatgcac tttgccctac ttcattcagc gcgtcgcaga cagcctggca
     2641 atcttttttg gtattgcaaa acaccacgca agaggatggc tgatgcaagc ttaataaccg
     2701 ttgcaacaga gggattttgc ctttgctgga tgtctcataa aattgttgtt caatgggtgg
     2761 caaagcatct gttgagtcaa tttcaatcgc caaaggatcg cgttgtactc gtccgctgat
     2821 tgcggcgatg gcttccggcc aggttgccga aaacagaagc gtctgtcgag atgcaggcgc
     2881 aaaacggatg acatcatcaa tggcatcgct aaatcccata tccagcatgc ggtcggcctc
     2941 atccatcacc aaagtattca gcgcatccag tgataccgtg cctttttgca agtgatccag
     3001 caaacgaccc ggcgttgcca cgataatatg cggcgcatgt tgcaacgaat cacgctgcat
     3061 accgaacggt tgaccaccgc acaacgtcaa aattttggta tttggcagaa aacgcgccag
     3121 ccgacgcaat tcacctgcca cctgatccgc cagttcacgc gtaggacaca gcactaaagc
     3181 ctgggtttga aatagcgacg catcaatttg ctgtaacaag ccgaggccaa aagccgccgt
     3241 tttgccgctg ccggttttcg cctgcacgcg aacatctttt ccggcaagga tcgccggaag
     3301 cgcggcggcc tgcaccggcg tcatggttaa ataacccaac tcattaaggt tcgtgagttg
     3361 ggcgggaggc aaaacattca gggtagaaaa agcggtcaca atctattctc gtggtcatcg
     3421 acgcaaagtt agcaggcgcg tatcctcgca gatctacgct cacgatgcga caatttaatc
     3481 ggttcttcat cgggtggtgg gtcaggcatg ggttgcgggc gagggatcgg atcgggcact
     3541 ggaacaggat cgctaggaat cggttcaggg acaggaattt gcaaataaat aagtgtcgtc
     3601 atatttccct ccggtcattg ggtggactct taaagggtag acgctgataa ataacaggca
     3661 aaaaaaagcc gactcatcaa agtcggcgtc gtacgaatca attgtgctat gcagtaattc
     3721 aaaaaaggaa gtaagacaat atggagcgca acgcgcaacg cccatcgctt gacgttgcat
     3781 tcacctgcaa tggagatatt gccctgaatg ggtagagagt ttattgactt cgctcaaact
     3841 ttgcggcgtt tttgtataca gacagccgga aaaattgctt ttgttacaac catttactac
     3901 ggtgcaacca taaagcaaca ccaccaataa gaacaactaa cagaatacaa aaaattgaaa
     3961 atccgaattg ccacccgccg ccagggatcc caccaaggtt gacgccaaat aaccctgtca
     4021 gaaaggtact gggtaaaaag accattgcca tcaatgacat tgtataggta cgacgagcta
     4081 aattttcctg catcacctga gcgatttcat ccgccatcac accagtccgt gctatacagg
     4141 cgtcgatttc gtcaaggccg cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt
     4201 ggtcatcgct catccacggc aaacgttcac tggcaagacg agcatagaca tcacgttgcg
     4261 gtgccatata gcgacgcatc acgattaatt gtttgcgcag cagagccagg aatccacgcg
     4321 gtggaatttg ctgatcaagg agattatctt caaggtcgat aattttatcg tgcagctgct
     4381 cgataaattc gctggaatga tcggtcaacg catcgcacac atccaccagc caccctccgc
     4441 aatcggtcgg acccgtgccc tcttccagat cgctcaccac atcgtccagc gccagcactt
     4501 tgcgttgtcg ggtcgaaaca attaaccgcc cgtccatata tacacgcatg gcgaccagtt
     4561 gatcggggcg ttcatcggtg ctgccgttta tacagcgcaa tgtaataagc gtgccttcac
     4621 cgagacggct gactcggggc cgtgtgctct cgcccgccag cgcatcacgt acgttattgg
     4681 gaagcagcgg tgttgtcgcc agccattggg cgctatcatg gtgtacataa ttaaggtgga
     4741 gccagcaggg atgcgcttca tcaatcacat ctgtattttc cagcggttta acgccgcctc
     4801 taccatccag catccaggca aatactgcat ccgggacatt aacgtccgat cccttaatcg
     4861 cttccacagt gcctccatca tcaacgcatt attttgtagt ctagccttct ggccctgtta
     4921 cgcaacatct catcacccca ttaccctgaa atgattaata aaattctgtc taaattgaat
     4981 acaaaaagca aaatgctttt ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca
     5041 ttatcagaat tatctgatca tatgacgtgg cttttttgcg atcggatagc aacaaaaatt
     5101 gataaaaata acgggatctc aatgattacg cacaacttca ataccctgga cttactcacc
     5161 agtcctgtct ggattgtttc gccctttgag gaacagttaa tttatgccaa tagcgcggcg
     5221 cgactgttga tgcaagacct cacgtttagt cagctacgaa ccggacccta ttccgtctcc
     5281 tcacaaaaag aactgccgaa atacctctcc gatctgcaaa accaacacga tattatcgaa
     5341 atcctcactg ttcagcgtaa agaagaggaa acagcattaa gctgtcggct tgttttgcga
     5401 gagctgacag aaacagaacc ggtgattatt ttcgaaggta tcgaagcgcc ggcaacgctg
     5461 ggtttaaaag ccagtcgctc ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc
     5521 ttttttctga ctaactctgc accaatgttg ttgattgacc cctcacgaga tggccaaatc
     5581 gtcgatgcta acctcgccgc gctcaatttc tatggttata accatgaaac gatgtgccag
     5641 aaacatacct gggaaattaa tatgctcggg cgtcgcgtca tgcctatcat gcatgaaatt
     5701 tcgcatttac ccggcggtca taaacctttg aattttgttc ataaactggc ggatggatct
     5761 actcgtcatg tgcagaccta tgccgggccg attgaaattt atggcgacaa gctcatgtta
     5821 tgtattgtgc atgatattac tgagcaaaaa cggctggagg agcagctgga acatgctgct
     5881 caccatgacg cgatgaccgg attactgaat cggcgacagt tttatcacat taccgagcca
     5941 ggccaaatgc agcacctcgc catcgcccag gattacagct tgttgctcat cgacaccgat
     6001 cgttttaaac acattaacga tctctatggg cattctaaag gtgatgaggt gttatgcgcc
     6061 ctcgcccgca ccctcgaaag ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc
     6121 gaagagtttg tcttattgct accaagaacc ccactggata ccgcgctttc gctggctgaa
     6181 actatccgtg taagcgtggc aaaagtgagt atttcgggct taccacgttt taccgtcagc
     6241 attggtgtgg cgcatcacga aggaaatgaa agcatcgatg aactgtttaa acgcgttgat
     6301 gatgctttgt atcgggcgaa aaatgatgga cgcaaccgcg tgctggcggc ataagccgcg
     6361 gatgcgtctc gagatcaacg gctgcgctta gcgtggcgct cccagttttc ttgcttcgcc
     6421 tgcgccgttt tacgtagtgc gacgtaacac gccccgctgc cgccatgatg cggtagcgcg
     6481 gtgcaatatg cctgaacatc atcaaattcg gtcagccagc gcgccacata gctgcggaca
     6541 atattggcat gcgatttatc atcccgtcct ttgccatgaa taatcagcac gttacgcaaa
     6601 ccatcagcca tggcttgttg aatgaaactg aacatcattt tgcggcactc ttccaccggc
     6661 tggcgcaaaa gattcaggct cgcctgttgc ggatatttac cactgcgcag cttatccagc
     6721 accccatgtt gcaacccttc ccgccgaaac tccagcggct gacttagtgg gatgatgtcg
     6781 agaaatccgg tggtgaggaa attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt
     6841 tggttacgcg ttggatgcca gtggacatcg gtagcacgtt tcagcggctg gacatcttcc
     6901 atggcgtcaa gaaacagcga tttgtcgtca aggttcatgt tacatcctcc cgcaattaag
     6961 agcgcgatat gataaccaga ccgggtcggt ccaacaacgt cttacccaaa attccagtaa
     7021 taagttccaa atattgccga tattttaagc aaaatgctta tgcatgatta ttcattcacg
     7081 atattaataa tgtaacttat attttcgtga aatctgtcac tgaagaaaat tggcaactaa
     7141 aggttaaaac cgttataaca cagtcaccgg cgcagaggag acaatgccgg acttaagacg
     7201 cggatgcact gctgtgtgta ctgtagagtc tggcggatgt cgacagactc tattttttta
     7261 tgcagtttta actttgcaga tagccgcatt ctcgccggaa ttgcgtgatt aatgacgcgg
     7321 tcagtggtgt ctggcgacta tcacgccgct gaatcaaata ataggccgct ttcggtaaaa
     7381 tttcgctaac cggcaacatc accagcccct gtccgtgcaa gggatcgcag cccatttctt
     7441 caggcagtat gctgagaaaa tcgctttttg ccaccagact gatacaggct gagaacgtct
     7501 cgcagactac accgacctgt ggcgtttgcg cctgatcgtc aagcaattca ctcaactgtt
     7561 tgtagtaact gccgtgtggc gtcggcattg tccagctgta atccagtaac tgtttgatcg
     7621 aacgggcacc aatggcgggg tgtcccgggc ggcaaaagat cgcgaattgc ttttccagta
     7681 atttctcaaa agtaaattcg tggtcgtacg gtccctgata ataggtattg atggtgaaat
     7741 ccaattctcc ctgacgcaat tcattaatca tcgacaccag ttgcccttcc ataatgcgta
     7801 cttttacctg cggatgctgc tgatgaaaac gagatatgac agctggcatc agactgcggg
     7861 aaatactggc ccccatgccg atattaatct gccctgccag ttgcccttgt cgttggcgaa
     7921 tttcctcttg cgctgcgcgc agctcttcaa gaataagact ggcgtgctga taaaaacttt
     7981 caccggcatc agttaacgtc acgcctttac tacggcgaaa aaagagttgc gccgctaatc
     8041 cttcttccag ctcctgaata gatttactca gtgccggttg cgacatattc aacattcggc
     8101 tcgctccgcg aatgctgccc tgacgagcca cttcaacaaa agcccgaatt tgatgaattt
     8161 ttacctgaaa agccatgacg ccaccgataa ccgttattta tcagaccaaa gaaactggca
     8221 tctactttaa tgcagatgat tgtgtcaggg taatttatga acggttaaaa ctgtgaaaaa
     8281 tcagttagtg ataagtaaaa actatcgcta cgtgaaccgg gtcacacttt ttactgatga
     8341 cgggaaaggt tatggagtct ttgaatcaat ttgttaattc gcttgcccca aaattatcgc
     8401 actggcgacg tgattttcat cactatgcag agtctggctg ggtggaattc cgcactgcca
     8461 cccttgttgc ggaagaattg cagcagctcg gctattcact ggcgctgggc cgcgaagtag
     8521 ttaatgaaag tagccggatg ggactacctg atgaactcac tctacaacgc gaattcgagc
     8581 gcgctcgtca acagggtgcg ctagcacaat ggattgcggt ttttgaaggt ggtttcacgg
     8641 gtatcgtcgc caccctggat accggtcgcc ccggtccggt tatggctttc cgtgtcgata
     8701 tggacgcgct ggatctcagt gaagagcagg atgtcagcca tcgcccctac cgcgacggtt
     8761 ttgcgtcatg taacgccgga atgatgcatg cctgtggtca tgatgggcat accgccattg
     8821 ggcttgggct ggcgcatacc cttaaacaat tcgagtccgg actacatggc gtcatcaaac
     8881 tgatttttca gcctgcagag gaaggtacgc gtggcgcgcg ggcgatggtc gatgcaggtg
     8941 tcgtagatga tgttgattat tttactgccg tgcacattgg cactggcgta cctgcgggca
     9001 ccgtggtgtg cggcagtgat aattttatgg caaccaccaa atttgacgcg cacttcaccg
     9061 ggaccgccgc tcacgcaggc gcaaaaccag aagacggtca caatgccttg ttggcggcag
     9121 cacaagcaac tcttgcactg catgcaatcg ccccgcacag cgaaggagct tccagagtaa
     9181 acgtgggcgt tatgcaggca ggaagcggcc gtaacgttgt tcctgcctcg gcgttgctga
     9241 aagtggaaac acgcggggcc agcgacgtca ttaatcaata tgtttttgac cgtgcacaac
     9301 aagcgattca gggcgcagca accatgtatg gtgtcggcgt tgaaactcgc ctgatgggtg
     9361 cagctaccgc cagttctcct tcgccgcaat gggtcgcatg gttgcaaagt caggcggctc
     9421 aggtcgcggg ggtcaatcag gccattgaac gtgttgaagc gcctgcgggt tccgaagatg
     9481 ccacattaat gatggcccgc gtgcagcacc atcaagggca agcctcctac gtggtgtttg
     9541 gcacacagct ggcggcaggt catcacaacg aaaaattcga ttttgacgag caggttctcg
     9601 ctattgccgt cgaaacgctg gcgcgcaccg cgctcaattt tccctggacg cgaggtatct
     9661 gatgcaggaa atctatcgtt ttatcgacga tgcgattgaa gccgatcgcc aacgttatac
     9721 cgatattgcc gatcaaatct gggatcatcc agaaacacgt tttgaagagt tctggtcagc
     9781 ggagcatctg gcttcggcgc tggaatctgc aagcttcacc gttacccgca acgtaggcaa
     9841 tatcccaaat gcatttattg cttcgtttgg tcaaggcaaa ccggttatcg ccctgctggg
     9901 agaatatgac gccctggcag gtttaagtca gcaagctggt tgcgcgcaac ctacatccgc
     9961 gacgcccggt gaaaatggtc acggttgcgg acacaatttg ctgggaaccg ccgcctttgc
    10021 cgctgcaata gccgtcaaga aatggctgga acaatatggg caaggcggca cagtgcgctt
    10081 ttatggttgt cctggcgaag aaggcggctc gggtaaaacg ttcatggtcc gcgagggggt
    10141 atttgatgat gtggatgcgg cactcacctg gcacccggaa gcctttgccg gcatgttcaa
    10201 tacccgcacg ctggcaaaca ttcaggcatc atggtgcttt aaagggatcg cagcacatgc
    10261 cgcgaattcc cctcatttgg gacgcagcgc ccttgatgcc gtaacgttga tgaccactgg
    10321 caccaacttc ctcaacgaac atattattga aaaagcgcgc gtacactatg ccatcacaaa
    10381 tagcggcggg atctcgccca acgtggtcca ggcgcaggca gaagtgcttt atcttatccg
    10441 cgcccccgaa atgaccgacg tgcagcatat ttatgatcgg gtcgccaaaa tcgccgaagg
    10501 tgcggcattg atgaccgaaa ccacggttga atgccgcttc gacaaagcct gttccagtta
    10561 tctcccgaat cgcaccttag aaaatgccat gtaccatgcc ctatcccatt ttggtacccc
    10621 ggaatggaac tccgaagaac tggcttttgc gaaacaaatt caggctacgc tcacccccaa
    10681 cgatcggcaa aacagtctga ataatatcgc cgcaaccggt ggcgaaaacg gcaaggcttt
    10741 tgcactacgt catcgtgaaa cggtactggc gaatgaggtc gctccatatg ccgccaccga
    10801 taacgtgctt gcggcatcga ctgatgtcgg cgacgtcagt tggaaactgc ctgttgccca
    10861 gtgtttcagc ccctgttttg ccgtcggtac accgctacat acgtggcaac tggttagcca
    10921 ggggcgaaca tctattgctc ataaaggaat gctgctggcg gcgaaaacta tggcagcaac
    10981 cacagtcaat ctcttcattg attcagggct attgcaagaa tgccaacaag agcatcagca
    11041 agttacggac acgcaaccgt atcactgccc tatcccgaaa aacgtgacac cgtcaccttt
    11101 aaaataacaa caacaacgca aacacaacaa ccgaggaatg cccatgagta tgtcatccat
    11161 accgtcgtcc tcccaatccg ggaagctcta tggctgggtc gaaagaattg gtaacaaggt
    11221 tccccatcct tttttgctct ttatctattt gataatcgta ctcatggtga cgacggcaat
    11281 tttgtcagcc tttggcgtca gtgcgaaaaa cccgaccgat ggtacgccgg tggtggtgaa
    11341 aaacctgctc agtgtggaag gattacactg gtttttaccc aatgtgatta aaaactttag
    11401 cggttttgct ccacttggag cgatcctggc gctggtttta ggtgccggcc tagcggagcg
    11461 cgtcggctta ctgccagcgc taatggttaa aatggcatcg catgttaatg cccgctacgc
    11521 cagttatatg gtgctgttta ttgctttttt cagccacatt tcttccgatg cggcgttagt
    11581 gatcatgcca ccgatgggtg cgctgatttt tctggcggtg ggcaggcatc cagttgcagg
    11641 tttactggct gccattgcag gcgtaggttg cggctttacg gctaatttac tgattgtcac
    11701 aaccgacgtg ttgctgtcgg ggatcagcac ggaagcggcg gctgcgttca atccgcaaat
    11761 gcacgtcagt gtaattgata actggtattt tatggccagc tccgtagtcg tactgacgat
    11821 tgttggcggc ttgataaccg acaaaatcat cgagccacgg ttaggtcaat ggcagggaaa
    11881 cagcgatgag aaactgcaga cattgaccga aagtcagcgt tttggtttac gcatagcagg
    11941 tgtcgtatcg ctacttttta ttgctgcgat cgcgctgatg gtgatcccgg aaaacgggat
    12001 attgcgcgat ccgattaatc acaccgtgat gccatcaccc tttattaaag gtatcgtgcc
    12061 actaatcatt ctttttttct ttgtggtttc gctggcttat ggcatcgcta cccgcacaat
    12121 tcgacgtcag gcggatttac cgcatttaat gattgaaccg atgaaagaga tggcgggatt
    12181 tatcgtgatg gtttttcccc tcgcccagtt tgtcgccatg tttaactgga gcaacatggg
    12241 gaaattcatc gccgtggggc tgaccgatat acttgaaagt tcagggctta gcggcatccc
    12301 ggcgtttgtc ggtctggcgt tgctttcctc tttcttatgc atgtttattg ccagcggttc
    12361 cgcaatctgg tcgattctgg cccccatttt cgtaccaatg tttatgctac ttggctttca
    12421 cccggcattt gcgcaaatcc tctttcgtat tgccgactca tccgtattgc ctttagcgcc
    12481 ggtatctcct tttgttccac tgtttcttgg attcctgcaa cgctacaaac cagacgcgaa
    12541 actgggtact tactattcgt tagtcttgcc ctatccgctt atctttttgg tggtatggct
    12601 gctgatgttg ctggcgtggt atcttgttgg tctgccgata ggtccgggga tttacccacg
    12661 tttgtcttaa gagagaacgg atgctgagat tacttgaaga aaaaattgcc acgccactgg
    12721 gtccactgtg ggtgatgtgc gatgagcaat ttcgcctgcg ggcggttgaa tgggaagagt
    12781 acagcgaacg catggtgcag ctgctggaca tccattatcg taaagaaggc tatgagcgca
    12841 tttctgccac caacccaggc ggtttaagcg acaagcttcg tgattatttt gccggtaatc
    12901 ttagcattat tgatacgctt cccactgcca caggggggac gccatttcag cgcgaagtct
    12961 ggaaaactct acgcactatc ccctgcgggc aggtaatgca ttacggccaa ctggctgaac
    13021 aattgggccg ccctggcgcg gcgcgtgccg taggtgcggc aaatggatcg aatcccatca
    13081 gcatcgtcgt accttgccat cgggttattg gtcgaaacgg caccatgacc ggatatgcag
    13141 gcggagttca gcgaaaagag tggttattgc gccacgaagg ttatcttttg ctgtaaacat
    13201 taaacaattt gtgccagctt gttcacactt ttatgtaaag ttacccttaa caacttaagg
    13261 gttttcaaat agatagacat atatttacat ctaatatcgg aattctctgc tgttaaggtt
    13321 tgcttagact tacttgctcc ctaaaaagat gttaaaattg acaaatatca attacggctt
    13381 gagcagacct atgatcccgg aaaagcgaat tatacggcgc attcagtctg gcggttgtgc
    13441 tatccattgc caggattgca gcatcagcca gctttgcatc ccgttcacac tcaacgaaca
    13501 tgagcttgat cagcttgata atatcattga gcggaagaag cctattcaga aaggccagac
    13561 gctgtttaag gctggtgatg aacttaaatc gctttatgcc atccgctccg gtacgattaa
    13621 aagttatacc atcactgagc aaggcgacga gcaaatcact ggtttccatt tagctggcga
    13681 cctggtggga tttgacgcca tcggcagcgg ccatcacccg agcttcgcgc aggcgctgga
    13741 aacctcgatg gtatgtgaaa tcccgttcga aacgctggac gatttgtccg gtaaaatgcc
    13801 gaatctgcgt cagcagatga tgcgtctgat gagcggtgaa atcaaaggcg atcaggacat
    13861 gatcttgctg ttgtcgaaga aaaatgccga ggaacgtctg gctgcattca tctacaacct
    13921 gtcccgtcgt tttgcccaac gcggcttctc ccctcgtgaa ttccgcctga cgatgactcg
    13981 tggcgatatc ggtaactatc tgggcctgac ggtagaaacc atcagccgtc tgctgggtcg
    14041 cttccagaaa agcggtatgc tggcagtcaa aggtaaatac atcaccatcg aaaataacga
    14101 tgcgctggcc cagcttgctg gtcatacgcg taacgttgcc tgatttttcc gcataactca
    14161 ctatccttct gtcatatcat taaatttttc tgatttattg atctggcaga aggttcatca
    14221 ctgtttcatt caccagatat gggttaatct tttaattaca aactgcgttg acagttgttg
    14281 taaggagacc ctgtatggct atgtatcaga acatgctcgt tgttatcgat cctaaccagg
    14341 acgaccaacc agcattgcgg cgagctgttt atttacatca acggattggt ggcaaaatta
    14401 aagccttttt gccgatctat gacttctcat acgaaatgac caccctgctc tccccggacg
    14461 aacgtaccgc tatgcgtcag ggcgtcatca gccagcgtac agcctggatc cacgagcagg
    14521 caaaatatta tctcaatgct ggcgttccca ttgaaattaa agtggtctgg cataaccgtc
    14581 ccttcgaagc catcattcag gaagtgatca gcggcgggca cgatttggtg ctaaaaatgg
    14641 cgcaccaaca tgaccgtctg gaagcggtga tttttacgcc aacggactgg catctgttac
    14701 gcaaatgccc aagcccggtg tggatggtga aagaccagcc gtggccggaa ggaggcaagg
    14761 cgctggtggc ggtgaatctc gccagtgaag agccgtatca taatgcgctc aatgaaaaac
    14821 tggtcaaaga gacgatcgaa ctggcagaac aagtcaacca taccgaagtt catctggttg
    14881 gcgcttatcc ggtaacgcca attaatatcg cgattgaact gccggaattt gatccgagcg
    14941 tttataacga tgccattcgt gggcaacatt tgctggcaat gaaagctctg cggcagaaat
    15001 tcggcattaa tgaaaacatg acgcacgtag aaaaaggtct gccagaagag gtgattcctg
    15061 atttggcgga gcacttacag gcggggattg tggttctggg cacggtagga cgcaccggta
    15121 tttcagcagc attcctcggc aacacggcgg aacaggtgat tgatcatctt cgctgcgatc
    15181 tgttggtcat taaacctgac cagtatcaga cacccgttga actggatgac gaagaagacg
    15241 attaacgact cttcaataac tgacaaggcc cgagttatcg ggcctatttt ttacttactg
    15301 ctcttcgccg ccaaggaaat aaattcccaa cgggattgcc agcaacacag taaagaccag
    15361 gctataaaca aaaatcatcg atgactgaat cacatacatt gatgtcgtcc actcagacag
    15421 cggaatattg tactgttcta taacgccgcc gatagtcgcg cgagtcacaa ccgacgccgc
    15481 aataatgaaa accgccaaca aaccaaagag aaatttcttc cctttcgctg acttcagttt
    15541 cgccataatc atgatgtaat ccttgtgcag acaaatttct tgttacctaa acgtaacaat
    15601 accatgacat ttcaccgttg tctgctctga aaacctcacc tcttagctgg atttacaatc
    15661 aacgcattga ttagttagct aatgttgcta cttccttccc tgactattga tgatctgaat
    15721 gaattgtcgc ggtaaaatag ccccgccagg ggtttccggc atgacgacaa ttatctggag
    15781 gagcctgata aaacaatgat cgctgaactg tttacaaata atgcgcttaa tctggtcatt
    15841 attttcggta gctgcgcagc attgattctg atgagctttt ggtttcgccg cggaaatcgt
    15901 aaaagaaaag gttttttatt ccatgcggtg caatttttaa tctacaccat aattatcagt
//
LOCUS       DAZGLJ010000104        15719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000104 DAZGLJ010000000
VERSION     DAZGLJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.104"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            47..589
                     /gene="yfaZ"
                     /locus_tag="ACHK49_002838"
     CDS             47..589
                     /gene="yfaZ"
                     /locus_tag="ACHK49_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO9263459.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            689..1891
                     /gene="yfaY"
                     /locus_tag="ACHK49_002839"
     CDS             689..1891
                     /gene="yfaY"
                     /locus_tag="ACHK49_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO9263460.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            1955..2416
                     /locus_tag="ACHK49_002840"
                     /pseudo
     CDS             1955..2416
                     /locus_tag="ACHK49_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(2457..3359)
                     /gene="rpnE"
                     /locus_tag="ACHK49_002841"
     CDS             complement(2457..3359)
                     /gene="rpnE"
                     /locus_tag="ACHK49_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO9263461.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(3553..4743)
                     /gene="glpC"
                     /locus_tag="ACHK49_002842"
     CDS             complement(3553..4743)
                     /gene="glpC"
                     /locus_tag="ACHK49_002842"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO9263462.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(4740..5999)
                     /gene="glpB"
                     /locus_tag="ACHK49_002843"
     CDS             complement(4740..5999)
                     /gene="glpB"
                     /locus_tag="ACHK49_002843"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO9263463.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(5989..7617)
                     /gene="glpA"
                     /locus_tag="ACHK49_002844"
     CDS             complement(5989..7617)
                     /gene="glpA"
                     /locus_tag="ACHK49_002844"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO9263464.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            7890..9248
                     /gene="glpT"
                     /locus_tag="ACHK49_002845"
     CDS             7890..9248
                     /gene="glpT"
                     /locus_tag="ACHK49_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO9263465.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            9253..10329
                     /gene="glpQ"
                     /locus_tag="ACHK49_002846"
     CDS             9253..10329
                     /gene="glpQ"
                     /locus_tag="ACHK49_002846"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO9263466.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(10371..10610)
                     /locus_tag="ACHK49_002847"
                     /pseudo
     CDS             complement(10371..10610)
                     /locus_tag="ACHK49_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10608..10712
                     /locus_tag="ACHK49_002848"
                     /pseudo
     CDS             10608..10712
                     /locus_tag="ACHK49_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10792..11442
                     /gene="inaA"
                     /locus_tag="ACHK49_002849"
     CDS             10792..11442
                     /gene="inaA"
                     /locus_tag="ACHK49_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO9263467.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(11496..11750)
                     /gene="yfaE"
                     /locus_tag="ACHK49_002850"
     CDS             complement(11496..11750)
                     /gene="yfaE"
                     /locus_tag="ACHK49_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO9263468.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(11750..12880)
                     /gene="nrdB"
                     /locus_tag="ACHK49_002851"
     CDS             complement(11750..12880)
                     /gene="nrdB"
                     /locus_tag="ACHK49_002851"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO9263469.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(12969..15254)
                     /gene="nrdA"
                     /locus_tag="ACHK49_002852"
     CDS             complement(12969..15254)
                     /gene="nrdA"
                     /locus_tag="ACHK49_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO9263470.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            15382..15525
                     /gene="ypaB"
                     /locus_tag="ACHK49_002853"
     CDS             15382..15525
                     /gene="ypaB"
                     /locus_tag="ACHK49_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO9263471.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         3655 a         3881 c         4289 g         3894 t
ORIGIN      
        1 ccgcagcttt gggaattttg attaattgct tgtgggaaag gggataatga aaaaaattgc
       61 gctagcaggt ctggccggaa tgctgttggt ttctgcatcg gtcaatgcaa tgagcatcag
      121 cggccaggcg ggtaaagaat acaccaatat tggtgtcggt tttgggactg aatcgacggg
      181 cctggcttta agcggtaact ggacacataa cgacgacgac ggtgacgtcg cgggcgtggg
      241 gctggggttg aatctgcctc tcgggccgtt aatggcgacc gttggcggaa aaggcgttta
      301 caccaacccg aattacggcg atgaaggtta tgccgcagcg gtaggaggtg gtttgcagtg
      361 gaaaattggc aacagcttcc gtttgtttgg cgagtattac tactctccgg attcgctctc
      421 cagcggtatt caaagttatg aggaagcgaa tgctggcgcg cgttacacca ttatgcgtcc
      481 agtcagtatt gaggcgggtt atcgctacct gaatctgtcg ggtaaagacg gtaaccgcga
      541 caacgccgtg gctgacggcc cgtatgttgg ggttaacgcc agtttctgat cccttccggc
      601 gcggcattct gtcgcgccat tcagtttctt actacgcctt atctcccttt gcgttatagt
      661 gattcgataa caaaagcagg agataaccat gttaaaagtg gaaatgctat ccaccgggga
      721 tgaagtgtta cacgggcaaa tcgttgacac taacgctgcc tggctggccg attttttctt
      781 tcatcagggg ctgccattat ctcgccgcaa tacggtgggg gataaccttg atgacttagt
      841 caccattctt cgcgaacgta gtcagcacgc cgatgtgctg atcgttaacg gcgggctggg
      901 accgaccagc gatgatttaa gcgcactcgc cgctgcgaca gcaaaaggtg aaggcatggt
      961 gctgcatgaa gcctggctca aagagatgga acgctatttc cacgaacgtg gacgagtaat
     1021 ggcaccgagc aaccgtaaac aagcggagct gcctgccagt gctgaattta tcaataaccc
     1081 ggtaggcacc gcctgtggtt ttgccgtgca gcttaatcgt tgcctgatgt tctttactcc
     1141 cggcgtaccg tcagaattta aggtgatggt cgagcacgaa atcctgccgc gtctgcgcga
     1201 gcgtttttct ttaccacagc cgccggtttg tctgcgttta actacctttg gtcgttcgga
     1261 aagcgatctg gcacaaagcc tggacactct acaactgccg ccgggcgtaa caatgggcta
     1321 tcgctcttca atgccgatta tcgaactgaa actcaccgga ccggcaagcg agcaacaggc
     1381 gatggaaaaa ctgtggctgg acgttaaacg tgttgccgga cagagcgtga ttttcgaagg
     1441 caccgaagga ctgcccgcgc agatcagtcg cgaattgcaa aaccgccagt tcagcctgac
     1501 gttgagcgag caattcaccg gtggtttatt ggctttgcaa ctttctcgcg caggtgctcc
     1561 attgctggcg tgtgaagtgg ttccttcaca ggaagaaacc ctggcgcaaa ctgcgcactg
     1621 gattacagaa cggcgggcca accattttgc cgggctggca ctggctgttt cgggtttcga
     1681 gaacgaacat ctcaactttg cgctagccac accagacggc actttcgctc tgcgtgtgcg
     1741 tttcagcact acgcgctaca gcctggctat ccgtcaggaa gtgtgcgcaa tgatggcact
     1801 gaatatgctg cgccgttggt taaacggcca ggatatcgcc agtgagcatg gctggattga
     1861 ggttattgag tccatgacct tatctgtctg attccgccac tctgtgccgg tttactgcac
     1921 cggcacattc cccctccggc aaaacttgtc caaccctccc tacggtgagc gtggtgttgg
     1981 ggccagtgtg gcacgggctg cgcgctgggg acgcattgag aattacatgg cgcaagtcaa
     2041 cgattcgctt tgtctgttag tgcaggtgga aagtaaaacg gcactggata acctggacga
     2101 gatcctcgac gtcgaaggga ttgatggcgt gtttattgga ccagcggatc tttctgcgtc
     2161 gttgggctac ccggataacg ccgggcaccc ggaagtgcag cgaattattg aaaccagcat
     2221 tcggcggatc cgtgctgcgg gtaaagcggc tggttttctg gctgtggctc ctgatatggc
     2281 gcagcaatgc ctggcgtggg gagcgaactt tgtcgctgtt ggcgttgaca cgatgctcta
     2341 cagcgatgcc ctggatcaac gactggcgat gtttaaatca ggcaaaaatg ggccacgcat
     2401 aaaaggtagt tattgatatc aaaggcccat ggggatcggc tgtgggcctg tgttaattag
     2461 tggttattcg ctgccagatc ggcttcgctt agctgggtgg ccgcgagcac ctggtcacga
     2521 tcaatgccct gttccagcat gcgcaaagca attttaatgg cttgttcatg cataccttcc
     2581 tgccagccaa tttgatgccc ttcctgccgt agccgttcag caatagtcat taatctctcc
     2641 ttttgtagtg gtgaacgttc ggcaatctcc tcaataaaac gggtgaaacg ggaggtatcg
     2701 ccgcattgca gcagataatt aaacagtgtt tgtagctggc tgtcattagt gaagcctcta
     2761 accaaaagcg tggtgatcct gtcgaccatg ccgattaaat cgcggtcgcg aatatgcttt
     2821 tgaatcagtt ccagcagagc tatacgccga tgttgcatga tctcatcgtc aggtacgatg
     2881 gtgatatcca ccaacggaaa agcttcggtg tacaactgcc gggcaagttg cggatcgtca
     2941 aactcatcca gccagttaag tgagtacggg tagggcgatg tttcgccatg ataaaacagc
     3001 aatggcacca ccagcggaac tctgtcatag cctttatcca ggtgacgctg catggcggca
     3061 gtggcatagc gcattagccg aaaagccata ttcttttctg cagagctttg atgttcaatc
     3121 acgcagtaga tatagccgtc accgtcgctg gtttccacgg accacaaaac atccgagtag
     3181 taagcgcgta aacttttttc aataaaacta gtgggttcca ggcgtaaggt ttgcaggttg
     3241 caaagcttgc gcagtggttc tggtaaatgt atttcgagaa aatcccgtgc ggtttcgggt
     3301 gtgaacataa aggttttaaa caccgcatca tgcggggagg atgttgttga ttcggtcatg
     3361 gttttccgtc acctgatatt tatgatgacg cgacattacg tctatcccgg gcctttgaca
     3421 tctgtcagtt tcagcctttg cgagacattt tcagaggaat tttcaaagcg ttgcaacgca
     3481 tacatggtgc gtggaatcgc gctcgcaaaa atgcccgata agcaatgtgc ttaccgggca
     3541 tcaaaaggtc ggttaagcca gcgcctgggc cagtagcgta atcggatgtt cgcagcgaag
     3601 gctcgtggac atctcaatct gccatttgca ggtttcgcag tcggtgatga ccagatccgc
     3661 gccgctctct tctatctggc ggaacagtgg cgcaccgatg gcttgtgagg tggggtagtt
     3721 ctcttttttg aaaccgtaag tacccgcaat accgcagcac tgggaatcca gcaccgttaa
     3781 ctcaagcccc gggattttac gcaacagctc cagggtgtag agcgtccagc ccattttttc
     3841 catatggcac ggagtgtgat aaaccacttt cagcggcagc ggtttcagcg gtaacgtttt
     3901 gccttcgtcc agcttgcgcc acagccagcg ggttgccagt tcgatatgat cgcgcaagcc
     3961 tttgttgtcg acattcagca cttccgggta ttcgtcgcgc agggcaaatg tacaggttga
     4021 ggaggtggca atcaccggaa tgccttttac tcccacagct tcgcggatcg actctacatt
     4081 cgtaattgcc tgtttgcgtg ctttatcggt aaagccgttg gcgattagcg gtacgccgca
     4141 gcatttttct ttgctgagca gttgtacacc ggtacccatt gcgttgagca ctttaattaa
     4201 atctttacct aactgcggat ggttgtagtt aacgaagcag ccgtgaaaga aagcgacctg
     4261 gtctttatat tgtgcttgct gagccgctat gctgcgatac cagcgacgaa acgtaccgaa
     4321 ggagtatttc ggtagcgtgc ggcgatgatc gatttttaac gccgcatcaa gcagctgccg
     4381 caccggtttc agcgaggtag cggtgttgac gattggtgcg aacggcgtgg aaacgctacc
     4441 catcaggtcg gtatgactca acacaaaatt acgcagcgac gggcgcgtgg tgtcatattt
     4501 tgcccgcgcg cgctggataa tatcgccaat cttcacatcg gacgggcagg cgacttcaca
     4561 acgtttgcag ttgatgcaat atttcagcgc ctcgtcatac agtgcgccat ctttcaaacg
     4621 cagacgctcg ccatccggcc cggcttgttt tggccctgga taaccgggat tcacccggct
     4681 caccgggcag gcggtggtgc agacggtgca cttaatgcag ttttcgaagc tggtgtcgtt
     4741 cattgttggc ctcctgcgcg ttgggcaatc tgttgagcgg catgtaaagc actgacggca
     4801 caaacaccgc cgccgcatcc ctgggcgatg ggatcaaatc cgcccagcac cgaaccgatg
     4861 gcaaacaggt tctcaatggt ttgccctgcc tgcgacgggc gtagcgtctg atcagtggtt
     4921 acaccgaact gctgccacgg ttgcggcgca aaaaaatcgc ccttatacca ttcaccccgc
     4981 gtggcggttt gtagcacatc aaggccgaga atcggctcgc gaatgccgtt acgttccgct
     5041 accagtccgc cactaaagaa actgccgctg gcgagaaccg cgaaacgtgg acgtagcgga
     5101 atatcggcgt gattgcgggt ccagatttca ttcactacgc catttttaca ggtcactttt
     5161 ttcacttcat cgcccggcat ccacacgcca ccctggcgca caaactggcg ctgtaactgg
     5221 ttttgcagac gaatgcccag cacggaaggc ggcagcgttg gcaaaagcat cagtgaacaa
     5281 ggtagttttt cattcaacca acgccacagt ttgtcatcgg ccaggccgaa gcaggcgggc
     5341 atcaggatca tttcgcaggt attggcgaca ggaataagcg catcaagtaa cagcggccag
     5401 ttttcttcat tatcaaggaa acgggcgata ttcaccgcgc gaaattcggt ggcgttattg
     5461 cgcaggacat ccagttccgg cagctctatt tctgcggttt caaccgaaag atccagttcg
     5521 cgcaacgaag ccgccgcaag atgcgcctgg aaatccatca gcccgcttat ccccactaca
     5581 catatcttct tcgcgggtag cggccagacg ggaacttctg gcgaacttaa ccaggttgca
     5641 cgcaaagtgc cgagcggggt aacgcgctga tgggccagtt ccacgctgcc ctgcaattgc
     5701 gcaccgcttt cagcgatcaa tgcctgcgcc tggcaagcga gatcgagcac gcgttgtggt
     5761 tcgagaagac tgtaaggatg ggctggtgcc tgctgacgca aagattccag tccactgtga
     5821 atgtctgtca ccggttgacc atcaggcaga tggctcagca aatccagcga accggatgag
     5881 aaatgcagcg cgctttgacc acgcgtgaca atggcacagc gcaggccgtg tttttgcagt
     5941 tgcaggccac agagtaatcc ggcgaggccg ccgcccataa tgacagtatc aaagcgcatc
     6001 tttctgctcc ttctccagac cacacaatcc ctgataaacc cagcgggtaa attcgctttc
     6061 gcgcagtgca tctccccagg cgatgggttg cacgcctttc cagcgttcgt taaggaaggt
     6121 ggaaagttgc tcgatagatt gcgcggacgt cgtgacgtta aaacgttgca gcagtccggc
     6181 agcgcggcag gcgcagagtt cgccctggca ggtacccatc cccacacggg tacgacggcg
     6241 taaatccagc aggctattaa cgtttaaatt ttctaccgcg tactgaactt cacccgcagt
     6301 taccgcttcg cactcacata ccaggctacg atgcagacgg ccttcgctca gccaggcagg
     6361 cgtgcgatcg ccatgacgat aaaccgcaga accgcgcagc ggggcaggca gggagatgac
     6421 tttacgcaag gtaacttcag cgggttcttg tgaaccaggc agtgccagat cggcagtcgt
     6481 acaggggcgc gtgttgccca gtttgcggca taccgcgtcg gtagcccatt cagccatcag
     6541 ccgataggtc atcagtttgc caccggtgat ggtgataaat ccgtccagac catcgcgttc
     6601 agcatggtcg agcagcacga tgccacggct gacgttacgt ccgctcgggt cgtcatcgct
     6661 ggcaaccagc gggcgcacgc cagaataggc ccgcaaaatg cgcgttttcg ccatcacggg
     6721 ggccagtttt tccccttcac gcagcagaat atcaacctct tctgccgtca ctcgattatc
     6781 gtcaatctcg ttgtaatcaa tacgtaaaga ggtggtgcca atcagcgaaa tggtatcgcc
     6841 aggcaccaga atatcggcgt cggaaggttt acggcagcgg ttgatcacat gctggttaat
     6901 gcggtgatcc atgatcagca gcgatccttt cgccgggaac atgcgaatgc gcagatcggc
     6961 atattcggca atgtgttgcc cccagatccc agcggcatta accacgacag gtgcatgaag
     7021 ggcctgagtt tcgccggtga gatggttacg tacacgaaca ccgcacaccg tcgcgccttc
     7081 acgaatcagc cccgtgactt catgagcggt aaggataacg gcaccgtgtt ctttggcatc
     7141 cagcatgttt gctgcggtca gacgaaatgg atcaacggtg ccatccggaa ctttcaccgc
     7201 gccaatcagt gccgggttaa cggcaggttc gataatgcgc gcttgctgcg ggtctatagc
     7261 ttctgcgctg atccctgctt cttcgcaggc gcgaataaaa gtggcctgga atgagaggtc
     7321 atcttccggc agggtgataa acaggccgtt ggttggttca acgcagtgac gtgcaatgcg
     7381 tttcaggatc tggttttcac taatgcattc gcgggccgat tccgcatcgg ttaccgcata
     7441 gcgcgcaccg ctgtgcagca ggccgtggtt acgcccggtg gcaccggttg cgatgtcgtg
     7501 gcgctcaacc aaaatcacgc gcagcccgcg cagggcacag tcgcgggcaa tcccggctcc
     7561 cgttgcgccg ccaccaatga taatcacgtc acttgattgc gagtcgcgag ttttcattgt
     7621 tatccctctg aagttcgttt tttaccattt agccatagta aaaacatgaa ttgtttgatt
     7681 tcgcgcatat tcgctcataa ttcgaaagtg aaacgtgatt tcatgcgtca ttctgaacat
     7741 tttgtaaatc ttatttaata atgtgtgcgg caattcacat ttaatttctg aatgttttct
     7801 taacatcgcg gcaactaaag aaacggcagg ttctcccact gaatcagtct gttaatcata
     7861 aataagacca cgggccacgg aggctttcaa tgttgagtat ttttaaacca gcgccacaca
     7921 aagcgcgctt acctgctgcg gagatcgatc cgacttatcg tcgattgcgc tggcaaattt
     7981 tcctggggat attctttggc tatgcggctt actatttggt tcgtaagaac tttgcgcttg
     8041 ctatgcctta tctggtcgag cagggattct ctcgcggtga tttaggtttc gcgctttcgg
     8101 ggatctcgat cgcttatgga ttttcgaaat tcataatggg ttcggtatcg gatcgctcga
     8161 atccgcgcgt tttcctgccc gcaggtttga ttctggcggc ggcagtgatg ttgtttatgg
     8221 gctttgtgcc atgggcgacg tcgagcattg cggtgatgtt tgtactgttg ttcctctgcg
     8281 gttggttcca ggggatgggg tggccgccgt gtggtcgtac tatggtgcac tggtggtcgc
     8341 agaaagaacg tggcggcatt gtgtcagtgt ggaactgtgc gcacaacgtc ggtggtggta
     8401 ttccgccgct gctgttcctg ctggggatgg cctggttcaa tgactggcat gcggcgctct
     8461 atatgcctgc tttctgcgcc attctggtgg cattattcgc ctttgcgatg atgcgcgata
     8521 ccccgcaatc ctgtggcttg ccgccgatcg aagagtacaa aaatgattat ccggacgact
     8581 ataacgaaaa agcggaacag gagctgaccg cgaagcaaat cttcatgcag tacgtgctgc
     8641 cgaacaaact gctgtggtat atcgccatcg ccaacgtgtt cgtttatctg ctgcgttacg
     8701 gcatcctcga ctggtcaccg acttatctga aagaggttaa gcatttcgcg ctggataaat
     8761 cctcctgggc ctacttcctt tatgaatatg caggtattcc gggcactctg ctgtgcggct
     8821 ggatgtcgga taaagtcttc cgtggcaacc gtggggcaac cggcgttttc tttatgacac
     8881 tggtgaccat cgcgactatc gtttactgga tgaacccggc aggtaaccca accgtcgata
     8941 tgatttgtat gattgttatc ggcttcctga tctacggtcc tgtgatgctg atcggtctgc
     9001 atgcgctgga actggcaccg aaaaaagcgg caggtacggc agcgggcttt accgggctgt
     9061 ttggttacct gggcggttcg gtggcggcga gcgcgattgt tggctacacc gtggacttct
     9121 tcggctggga tggcggcttt atggtaatga ttggcggcag cattctggcg gttatcttgt
     9181 tgattgttgt gatgattggc gaaaaacgtc gccatgaaca attactgcaa aaacgcaacg
     9241 gaggctaatg gcatgaaatt gacgctgaaa aaccttagca tggcgatcat gatgagcact
     9301 atagtcatgg gaagcagtgc aatggcggcg gacagcaacg aaaaaatagt catcgcccat
     9361 cgcggtgcca gtggatattt gccggagcat acgctgccag caaaagcgat ggcgtatgcg
     9421 cagggagcgg attatctgga acaggatttg gtgatgacca aagacgacca tctggttgtt
     9481 ctgcatgacc attatctcga tcgtgttact gatgttgccg atcgtttccc ggaccgggcg
     9541 cgcaaagacg gtcgttacta cgcgatagat ttcacgctgg atgaaattaa gtcgctgaaa
     9601 tttaccgaag gtttcgatat tgaaaacggt aaaaaagtgc agacttatcc ggggcgtttc
     9661 ccaatgggta agtccgactt ccgggtgcac acctttgaag aagagattga atttgttcag
     9721 gggttaaatc actctaccgg gaaaaatatc ggtatttatc ctgaaatcaa agcgccgtgg
     9781 ttccatcatc aggaagggaa ggatattgcg gcaaaaacgc tggaagtgct gaagaaatat
     9841 ggttacaccg gtaaggacga caaagtttat ttgcaatgtt ttgatgctga tgagctgaag
     9901 cgtattaaga atgagctgga acctaaaatg ggcatggatc tcaatctggt acagttgatt
     9961 gcctataccg actggaatga aacgcagcag aaacagccgg atggaagctg ggttaattac
    10021 aactacgact ggatgtttaa gccgggtgct atgaaacagg tggcggaata tgccgacgga
    10081 atcgggccgg attaccatat gttgattgag gaaacatcgc agccgggtaa tatcaaactc
    10141 actggcatgg tgcaagatgc tcagcagaat aaactggtag tgcatcctta taccgtgcgg
    10201 tcagataaac tgcctgaata cactactgat gtgaatcagt tatatgatgc tctgtataac
    10261 aaagcgggtg taaatgggct gtttactgat ttccctgata aggcagtaaa attccttaat
    10321 aaagagtaat gctactgcag gatggcgttc atgcgccatc ctgtctcaca tcaaattttt
    10381 ccattcacca cgagttgttc cgtacacgtt tgtagtaaaa acagtggact gccttctacc
    10441 ggcttttctt tagccaacaa cgaaatctgt cgatagaagg ttggttcgtg aggaacggaa
    10501 cacaattcac ctgcaatgct tttcagcgtt aactctggtt gtaaggcgat gcctaatccc
    10561 tgtcgaataa acttcatcgc tgtatccgac tgattaaatt cacattttat ctactgggcg
    10621 cactattagt ggtagtggct ggtggaatct atgcgctgca aatgaagggt aaaagcggtg
    10681 tcacgctagt agtggcaaaa gaaattcatt aatacgacac gtttcattaa gattttcctc
    10741 aggtaaccac ctatagtcat tgtcgttaat aaaaatgaca ggcgataggg tatggcagtt
    10801 tcagcaaagt acgacgaatt taatcactgg tgggcaacag agggcgactg ggttgaagag
    10861 cctaactatc gccgtaatgg tatgagcggg gtgcaatgcg tcgagcgcaa cggcaaaaag
    10921 ctgtatgtaa agcgcatgac gcatcatctg tttcattccg tacgttatcc gttcggccga
    10981 ccaacgattg tccgtgaggt tgcggtaatt aaagaactgg aacgggccgg tgtcatcgtg
    11041 ccgaagatcg tttttggtga agcggtgaaa attgagggtg aatggcgagc gttgctggtg
    11101 actgaagata tggcggggtt catcagcatt gctgactggt atgcccagca tgcagtatcg
    11161 ccttattctg acgaagtacg gcaagccatg ttgaaagcag tggcgctggc gtttaagaaa
    11221 atgcatagca ttaatcgtca gcatggctgt tgttatgttc gccatatcta tgtgaaaaca
    11281 gaaggtaatg cagaagctgg ttttcttgac ctggagaaaa gtcgtcgccg cttgcgtcgc
    11341 gataaagcga ttaatcatga cttccgccag ctggaaaaat atctggagcc aatccccaaa
    11401 gcggactggg agcaggtgaa agcgtattac tacgccatgt aatcgttgta ggccagataa
    11461 gacgcataag cgttgtatct ggcactacaa ccaattcaca tctcgatttc aatatcgcct
    11521 tttgcccggc aacaacaggg caaaatttcc cccggctgaa taaaggctaa cggttcggca
    11581 atccagtcaa cctgacctgc aaccagacgg gtgcgacagg agccgcagta accttcgcga
    11641 cactgatact caaccgccac attgtgggat tccagcgccg ccagaaggga agggtgttca
    11701 tcctggcaca gcagttgtgt gccagtgatg cgcagggtaa cgcgggccat cagagttgga
    11761 agttactcaa atcgtcggtg tccacttccg cgtcaatctg cccgaccaga taagaactga
    11821 cttccacttc ctgcggagca acctgcacgt tatcagacac cagccaggtg ttgatccatg
    11881 ggatcgggtt ggagcgcgtc tggaacggca gatccaaacc gactgcctgc atacggatat
    11941 tggtgatgta ttcaacgtac tggcagagaa tgtctttatt cagaccaatc atcgaaccgt
    12001 cgcggaacag ataatccgcc cagtctttct cctgttgagc tgcctgaaca aacaggtcat
    12061 agcactcctg cttacactct tcggcaattt ccgccatctc aggatcgtcc gcgccgctgc
    12121 gcagcagatt cagcatatgc tgggtgccgg tcaggtgcag ggcttcgtcg cgggcaatca
    12181 ggcgaataat tttggcgttg ccttccatca attcgcgttc tgcaaatgcg aaggaacaag
    12241 caaagctgac gtagaaacga atcgcttcca gcgcgttaac gctcatcagg cagagataca
    12301 gttttttctt cagctcgcgc aggctaacgg tcacagtttt accgttaacg gtgtgggtac
    12361 cttcgcccag cagatgccag tagctggtca tttcgatcag ctcatcgtaa tagctggaga
    12421 tcccttccgc acgtttctgg atctgctcgt tggtgacgat atcgtcaaac acaacagacg
    12481 gatcgttaac gatattacga atgatatgag tataggaacg ggaatgaatc gtttctgaga
    12541 acgcccaggt ttcgacccag gtctccagtt ccgggatcga gatcagcggc agcagtgcca
    12601 cgttcgggct acggccctga atggaatcca gcagcgtctg atatttcagg ttgctgataa
    12661 agatgtgttt ttcgtgttca ggcagtgcct ggtaatcgat acggtcgcga gaaacgtcaa
    12721 cttcttccgg acgccagaag aaagagagct gcttttcgat cagcttttcg aagatgtcat
    12781 atttttgctg atcgtagcga gccacgttga ccggctgacc aaagaacatc ggttctttga
    12841 gctgatcatt tttcgtctgt gaaaaggtgg tatatgccat gagtgtgtcc tgtggtttta
    12901 ttacccctca ccctaaccct ctcccgcaag gggagagggg acggtttggt gcaaaggctg
    12961 ggggattatc agatcttaca tgcgccgctt tcgcagccat cgtcctggat tgacggtacc
    13021 agatcgtcct gcgcgtcttc agcgccgtca cgggtgttct gataatacag cgttttgacc
    13081 ccgaatttgt aggcggtgag caggtctttc agcaactgct gcatcggcac ttttcctgac
    13141 gggaaacgtg acggatcgta gttggtgtta gcagagatcg actgatcgat aaatttctgc
    13201 atgataccta ccagttgcag gtagccatcg ttacccggca tttcccacag cagctcatag
    13261 gcgtcgtgca ggtgctcgta gtccggcacc acctggcgca aaataccgtc tttcgacgct
    13321 ttgatgctga cgtaaccgcg cggcggttca ataccgttag tggcgttaga gatctgtgaa
    13381 gaagtctctg acggcatcag ggcagaaagc gtggagttac gcaggccgtg cgttttgatt
    13441 gattcacgca atgcttccca gtcgtaatgc agcggctcat tagcgatggt atccagatct
    13501 ttcttatagg tatcgatcgg caggatccct ttcgcgtaag tggtttcgtt aaaccacggg
    13561 cacgcgcctt gctcttttgc cagctcgtta gacgctttca gcaggtaata ctgaatcgct
    13621 tcgaaggttt tatgcgtcag gttgttggcg ctgccgtcgg agtaacgcac accgtgcttc
    13681 gccaggtagt aagcgaagtt gatcacacca atacccagcg tacgacgacc catcgctcca
    13741 cgtttggcgg ccgggatcgg gtaatcctga taatccagca gcgcgtcaag tgcacgaacc
    13801 gccagaattg ccagctcttc cagttcatcc aggctattaa ttgcgcccag gttgaaagca
    13861 gacagcgtac acagcgcgat ttcaccgttc tcgtcgttga cgtcgttcag cggtttggtc
    13921 ggcagggcta tctccaggca caggttagac tgacgcactg gcgcgatggc cggatcaaac
    13981 gggctatggg tattgcagtg gtcaacgttc tgaatataga tacgaccggt agacgcacgt
    14041 tcctgcatca tcagcgagaa cagctcaacg gctttcacac gctgcttgcg gatgctgtcg
    14101 tctttctcat atttggtata caggcgttca aactcttcct gatcggcgaa gaatgcgtcg
    14161 tacagccccg gtacgtcgga cgggctgaac agggtgatat cttcaccttt cagcaggcgg
    14221 gtatacatca gtttgttgat ctgtaccccg tagtccatat ggcgcacgcg gttgccttcc
    14281 acaccacggt tgtttttcaa caccagcagg ctttccactt ccagatgcca catcgggtag
    14341 aacagcgttg ccgcaccgcc gcgcacgccg ccctgagagc aggatttcac cgctgtctgg
    14401 aaatgtttgt agaacggaat acagccggta tggaacgctt caccaccgcg aatcgggcta
    14461 cccagcgcac gaatacgccc ggcgttgatg ccgatcccgg cacgctggga aacgtatttc
    14521 acaatcgcgc tggaggtagc gttgatggaa tccagactgt caccgcactc gatcagtacg
    14581 caggagctga actgacgagt cggggtacgc acgccggaca tgattggcgt cggcagcgaa
    14641 attttaaatg tggaaaccgc gtcgtaaaaa cgcttcacat attgcaggcg cgtttcacgc
    14701 gggtagttcg agaacaggca cgcggcaacc agaatataaa ggaactgggc gctttcgtag
    14761 atttcgccgg tcacgcggtt ctgtaccaga tatttgcctt ccagctgctt aacggcagca
    14821 taagagaagg tcatatcacg gtcgtgatcg ataaacgtgt ccatctgctt gaactcttct
    14881 tccgtgtagt cttccagcag atgattatcg tatttgccca tctcgaccat tttcaccacg
    14941 tggtcgtaca gcgcaggcgg ctcaaactgg ccgtaggctt ttttacgcag gtggaagatc
    15001 gccaggcgcg cggcgagata ctgataatcc ggcgcatcac gggagatcag gtctgcggcc
    15061 gccttgataa tggtttcgtg gatgtcagag gtcttgatac cgtcataaaa ctgaatgtgg
    15121 gagcgcagct cgacctggga aatcgaaacg ttatgcagtc cttctgccgc ccaatccaga
    15181 acgcgatgga ttttgtcgag attgatgcgc tctgtgctac cgtcgcgctt tgtcaccagc
    15241 agattctgat tcatgtatgt cgtacctgtt tttggaattc tttccgcaat agtttcatgc
    15301 agactacctg tagtacggga agctgcttag aacactatat atagggggta tgtttgggat
    15361 tcactgcaag atagtgtgaa aatggccctc ttgcaagtgc ataactttgt ggataactca
    15421 ggaaggaaaa agtggctttc gcgcacctta ggtcagacaa ggtgtccggg aaagtcaatg
    15481 ggaagagaaa aatttgttaa aaataactgt tcggcgattt cttgaacggc agaataggtt
    15541 ggataaattg atatagatgg tttattaatc tttttgctaa tgattgcaaa aattgatcta
    15601 agcacgagtc atcacataat ctgatgtatc agttgtggtc aatatataag cgaatgtaag
    15661 cgattgttga tgtcgaaatg gttgttttct attcggttat tgttaaatgc gaagccatt
//
LOCUS       DAZGLJ010000105        15695 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000105 DAZGLJ010000000
VERSION     DAZGLJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.105"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1347)
                     /gene="uidB"
                     /locus_tag="ACHK49_002854"
     CDS             complement(<1..1347)
                     /gene="uidB"
                     /locus_tag="ACHK49_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO9263472.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQ"
     gene            complement(1344..3155)
                     /gene="uidA"
                     /locus_tag="ACHK49_002855"
     CDS             complement(1344..3155)
                     /gene="uidA"
                     /locus_tag="ACHK49_002855"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO9263473.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     AEQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(3544..4134)
                     /gene="uidR"
                     /locus_tag="ACHK49_002856"
     CDS             complement(3544..4134)
                     /gene="uidR"
                     /locus_tag="ACHK49_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO9263474.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAERMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(4355..5122)
                     /gene="hdhA"
                     /locus_tag="ACHK49_002857"
     CDS             complement(4355..5122)
                     /gene="hdhA"
                     /locus_tag="ACHK49_002857"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO9263475.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(5234..6262)
                     /gene="malI"
                     /locus_tag="ACHK49_002858"
     CDS             complement(5234..6262)
                     /gene="malI"
                     /locus_tag="ACHK49_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO9263476.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            6437..8029
                     /gene="malX"
                     /locus_tag="ACHK49_002859"
     CDS             6437..8029
                     /gene="malX"
                     /locus_tag="ACHK49_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO9263477.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVLALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            8039..9211
                     /gene="malY"
                     /locus_tag="ACHK49_002860"
     CDS             8039..9211
                     /gene="malY"
                     /locus_tag="ACHK49_002860"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO9263478.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRLLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            9315..10316
                     /gene="add"
                     /locus_tag="ACHK49_002861"
     CDS             9315..10316
                     /gene="add"
                     /locus_tag="ACHK49_002861"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO9263479.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(10351..11391)
                     /gene="ydgJ"
                     /locus_tag="ACHK49_002862"
     CDS             complement(10351..11391)
                     /gene="ydgJ"
                     /locus_tag="ACHK49_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO9263480.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            11634..11759
                     /gene="blr"
                     /locus_tag="ACHK49_002863"
     CDS             11634..11759
                     /gene="blr"
                     /locus_tag="ACHK49_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO9263481.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            12032..12247
                     /gene="ydgT"
                     /locus_tag="ACHK49_002864"
     CDS             12032..12247
                     /gene="ydgT"
                     /locus_tag="ACHK49_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO9263482.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            12333..12773
                     /gene="ydgK"
                     /locus_tag="ACHK49_002865"
     CDS             12333..12773
                     /gene="ydgK"
                     /locus_tag="ACHK49_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO9263483.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            12850..13431
                     /gene="rsxA"
                     /locus_tag="ACHK49_002866"
     CDS             12850..13431
                     /gene="rsxA"
                     /locus_tag="ACHK49_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO9263484.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAIGFSLVMVLFAAIRERLAV
                     ADVPAPFRGHAIALITAGLMSLAFMGFSGLVKL"
     gene            13431..14009
                     /gene="rsxB"
                     /locus_tag="ACHK49_002867"
     CDS             13431..14009
                     /gene="rsxB"
                     /locus_tag="ACHK49_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO9263485.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     ITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            14002..>15695
                     /gene="rsxC"
                     /locus_tag="ACHK49_002868"
     CDS             14002..>15695
                     /gene="rsxC"
                     /locus_tag="ACHK49_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO9263486.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIAR"
BASE COUNT         3731 a         4080 c         3904 g         3980 t
ORIGIN      
        1 ctgctgcact tttttacgat tatcaatttc aaccacgatt tctttgaatt ttttatccgt
       61 gagcggataa aaccagataa taacgaatgc cagtagcata aatccgcaag gtactaaggc
      121 aattgatgtg cggatgccca taataacttc cggcgtttgc acctgattgg cgatatatcc
      181 gcttaatccc aaaataaagg caggaattga acctccgatt gcctgaccgc atttacgggt
      241 aaatgagaat agtgaatagg tgagcccttc aattcgcacg ccggtcaggt attcaccgta
      301 ttctacggta tcagcttcca gtgcccacat cacagtcata gtaacgccct gaccaattga
      361 agcgatggcc aacgcaacca acgccaccgg cagtgaccag acggaaaccc agaagaatag
      421 cagataaccg caggttccca gcaaagcgcc aatcaggaag gtattctttt taccgatcct
      481 cgcgaccatc cccggcacca gcggtgccga tgccacagta ccaaccaggt tttgcaccag
      541 taccagcaca gtgaacagcc cggtatcatt taacacatag cgcacgtaga acaacgacga
      601 ggcgctgacc gcgaaggtcg aaatcagcac acacagcgca ccgatgcaca acataaacag
      661 cgggcgattc cgtttcaggg tttgcagact gatcttcaat gacggctgcg ccacgatacg
      721 taccacattc tcacgcgtcg atttgaagca gatgaagtaa agcaccattc cggcaatcgc
      781 cagcacaatc gtccagaaat ggtataccga caccatctct tccgggctgg agttcttaat
      841 gctcggtcct atcagaaatg ccaggcagac aaaggttaat gaagcggcaa tcccgcgagc
      901 cgcgcccaga cgggcgcggg attgtggttg ttgggtcatc gcggtagcaa gtgaaccata
      961 aggaatattc accaggctgt agcaaagccc gaggcccatg taggtcaaat atgcatacac
     1021 cactttgcta ccatggctcc agtcggtcgg cacccagaat accagcacgc tgaagatcat
     1081 taacggcgca gtaccgaaga gtaaaaacgg gcggaatttt ccccagcggg tattcacact
     1141 gtccaccact cgtccggcaa agacgtcggc gaaggcatcg aatacccgca ccagtaacag
     1201 catggtgccc gccgcagcgg caccgacgcc agcgacgtcg gtgtagtaac tcaataggaa
     1261 gagcgccccc attgcgaagg cgaagttatt ggcgacgtca ccgaggctgt agccgacgat
     1321 ggtgcgccag gagagttgtt gattcattgt ttgcctccct gctgcggttt ttcaccgaaa
     1381 ttcatgccag tccagcgttt ttgcagcaga aaagccgccg acttcggttt gcggtcgcga
     1441 gtgaagatcc ctttcttgtt accgccaacg cgcaatatgc cttgcgaggt cgcaaaatcg
     1501 gcgaaattcc atacctgttc accgacgacg gcgctgacgc gatcaaagac gcggtgatac
     1561 atatccagcc atgcacactg atactcttca ctccacatgt cggtgtacat tgagtgcagc
     1621 ccggctaacg tatccacgcc gtattcggtg atgataatcg gctgatgcag tttctcctgc
     1681 caggccagaa gttctttttc cagtaccttc tctgccgttt ccaaatcgcc gctttggaca
     1741 taccatccgt aataacggtt caggcacagc acatcaaaga gatcgctgat ggtatcggtg
     1801 tgagcgtcgc agaacattac attgacgcag gtgatcggac gcgtcgggtc gagtttacgc
     1861 gttgcttccg ccagtggcgc gaaatattcc cgtgcacctt gcggacgggt atccggttcg
     1921 ttggcaatac tccacatcac cacgcttggg tggtttttgt cacgcgctat cagctcttta
     1981 atcgcctgta agtgcgcttg ctgagtttcc ccgttgactg cctcttcgct gtacagttct
     2041 ttcggcttgt tgcccgcttc gaaaccaatg cctaaagaga ggttaaagcc gacagcagca
     2101 gtttcatcaa tcaccacgat gccatgttca tctgcccagt cgagcatctc ttcagcgtaa
     2161 gggtaatgcg aggtacggta ggagttggcc ccaatccagt ccattaatgc gtggtcgtgc
     2221 accatcagca cgttatcgaa tcctttgcca cgcaagtccg catcttcatg acgaccaaag
     2281 ccagtaaagt agaacggttt gtggttaatc aggaactgtt cgcccttcac tgccactgac
     2341 cggatgccga cgcgaagcgg gtagatatca cactctgtct ggcttttggc tgtgacgcac
     2401 agttcataga gataaccttc acccggttgc cagaggtgcg gattcaccac ttgcaaagtc
     2461 ccgctagtgc cttgtccagt tgcaaccacc tgttgctccg catcacgcag ttccacgctg
     2521 acatcaccat tggccaccac ctgccagtca acagacgcgt ggttacagtc ttgcgcgaca
     2581 tgcgtcacca cggtgatatc gtccacccag gtgttcggcg tggtgtagag cattacgctg
     2641 cgatggatcc cggcatagtt aaagaaatca tggaagtaag actgcttttt cttgccgttt
     2701 tcgtcggtaa tcaccattcc cggcgggata gtctgccagt tcagttcgtt gttcacacaa
     2761 acggtgatac gtacactttt cccggcaata acatacggcg tgacatcggc ttcaaatggc
     2821 gtatagccgc cctgatgctc catcacttcc tgattattga cccacacttt gccgtaatga
     2881 gtgaccgcat cgaaacgcag cacgatacgc tggcctgccc aacctttcgg tataaagact
     2941 tcgcgctgat accagacgtt gcccgcataa ttacgaatat ctgcatcggc gaactgatcg
     3001 ttaaaactgc ctggcacagc aattgcccgg ctttcttgta acgcgctttc ccaccaacgc
     3061 tgatcaattc cacagttttc gcgatccaga ctgaatgccc acaggccgtc gagttttttg
     3121 atttcacggg ttggggtttc tacaggacgt aacataaggg actcctcatt aagataaaaa
     3181 tactggtcaa cctttaatct gattagttaa ccaattctgt gttgcacaca gttatctgtg
     3241 agaaacccat ggaagagaaa tggaaataaa aagaactgac accgcgatca aaaaaccaaa
     3301 aactgcaata cccacagcgt gatattgatc gcattaatct ttaaaacggc gttgacagaa
     3361 gccataaaat aagcaaccga acgttcgttc gagtatattg ggaacgataa actctcatga
     3421 catatggcta cggtgaatat ttttgggggg gaattctccg atcccggcta gattgtattt
     3481 tgtacaggga aattaaagta tgaaacgcgg gcacagttat caccagcccg cgaaatgaga
     3541 ggatcaggat gcggttaaga taccgccaat catcgcgcgt aatccctgag caaacgacgc
     3601 ttcacgcgcc agatcttccg cttcgatatc ggccagtaca ccgtaggtca gatccagcag
     3661 taaacgtgaa gtcattgacg tgttgatgtc tgggcttatc tcgccttttt gctgggcatc
     3721 acgcatccgc tcggcaacaa attcagtgat cgtcatatgc ttatttttaa gcatggcggc
     3781 gacctgtggg ttacgcatcc cttccgccat aatttcgacc accagcgccc gttgcccaaa
     3841 ggcttcatgg gtgagagaga caatagactc gaccatatag tcaacaaaat gaatcccttc
     3901 aatcggttcc cggaaacggg ccagcgccct ctcctggtcc tgtaagataa tcgcctgaat
     3961 caaggcttct ttggagatga aatggtgata gagcgtcccg ggactaatgg cgcaggattt
     4021 acagatggct ttcatcgagg cactgtgaaa tccattttct gaaaaaatct ctctggcagc
     4081 attgaggatc cgggtccgtg tcggttgtgc ttcagtctgc atgttatcca tcataaaacc
     4141 attatagtta gcgtgaataa ctagtttacc gcgttgtcag gtgagcgcct atcattgctg
     4201 ttgaatagtg cgaaggcaca ctctattcgt atacttttca ctctgaaatt ggttaaccaa
     4261 tagccgtgat ttggctgcca gttatttagc agagacgttg atgagtttat cccggtaaac
     4321 aacacgcacg gttgtttacc ggtccgttag tgtattaatt gagctcctgt accccaccac
     4381 cggagacggt gagaatttgt ccgcttaccc agctcgcagc aggcgagcaa aggaacagcg
     4441 ctgcgttagc aatatcttgc ggttggccca gacgtctgat cggcgtgtgc tgtaacattt
     4501 tttgttcaat ttctggtgta ataacggatt tcagggcatc ggttaatatt gcccccggcg
     4561 caatgccatt tacccgaata tttttttcac ccaggtcaaa cgccatattt ctgaccagat
     4621 gactggctgc agctttagat gatgcatagg aagtcatgtt tatattttta ttttctgccg
     4681 ccatagaagt gatggtcaga ataacgccac cgccattttt ttccatttct ggcgcaacaa
     4741 gttgtgacag atggaaaaaa gaaaacacat tcagttcata agcacggcga aaatccgcca
     4801 ttggcatatc aaacggttta ggtccaccgc caccggcgtt gttaaccaga atatcaactt
     4861 tacccagctt actgatagca aagtctgcca gtgcagagag ttcctgttcg gaagtaatat
     4921 cacaacggca ggcaaatgcc tgaccaccca gttgttgaat ttcgtctaca acatggttag
     4981 ctgcgtcggc gttaatatca ctgaccacca cagatgcgcc agctgtcgcg aatgtaatgg
     5041 cgatttcttt accaatacct gcacccgcac ctgtgatgat ggcgcatttt ccgtcgagtc
     5101 tcaggttgtc agaattaaac acataaacct cctgtgaact tcagtaaaaa taaaaggcac
     5161 cgcctgtgtg ccacattttt tatcattaac cttgaggaaa ggatactacc aggtatagca
     5221 gataaagaga ggattatttc gccgcaatga gccgggcggg aataataaga ttgcgtgaat
     5281 gcgtctcttc atgggtgatt ttttgcatca tgcgatccgc aagtgtgata ccaagttccc
     5341 gcgctggcgt gctggcccag gtaacaggta tatcatcaag tgtggttggt gtcgcatcgg
     5401 taaatgccgc cagcgaaacc tgttgctcaa agtaacgatc gactccgctt tccccgcttt
     5461 gccgccctgc tttgagcaaa ccaaaccatg cccccatcgc aatagtttcg ttatagcaaa
     5521 ccacggcact gatggtcggg ttatgacgta aaagcgccgt gatagcttcc gcggcttgct
     5581 tctggctgga agtgcactcc aacacccaat cgctatgaaa cggcaggcca aattttagta
     5641 gagttgcaca atagcccccc acccgttctg cacgggttaa tgaggaactt tgccctccca
     5701 gccaggcgat ccgctgatgc ccattgcgaa tgagatgctc cgtcaacaac tgtgcagcct
     5761 gcatgttgtc cgggcgaacc gtatcaacat catcaagata actggcacgg gaagcgaaaa
     5821 tcaccgggat agctttttct tctgccatcc gtcgcaggtc atcgctgctt cctgcagccc
     5881 cggcaattac cacaccatcc acaccctgat tcagtaacag tgaaaaccgc tgtgccagct
     5941 gctcgccgtc tttaccgccg tgaagcaaaa aaaccatccg tccctgcgct tccagagctt
     6001 ccgtcaatcc ggccgtcaat tcggcgtaaa acggcgcaga taaatcacgg acgatcaaac
     6061 caatgacgcc gctttgcccg ccgcgcagcg ccgacgcctg gcgattgcgc acaaatccca
     6121 gctcttcaat ggcggcgtta acgcgttctc ctgtggcggt agagattcgc cctttgccac
     6181 taagcaccag cgaaacggta cttaccgaca cgcccgcagc cagcgcaaca tcatgaatgg
     6241 ttattttttt ggcggtagcc atgcagatga cctactccct gattatgtga cagataaaac
     6301 gttttacctt ttatttatct tatactcgct attatcgttg cgtaatgtga tttatgcctc
     6361 actaaaattt gataaaacgt tttatcttct cgcgcaattt actgaatcca gattgttctc
     6421 tacgaggagt cgttttatga cggcgaaaac agcaccgaaa gtcacgctgt gggagttctt
     6481 ccagcagtta ggcaaaacct tcatgttacc cgtggcatta ttgtcgttct gcggcattat
     6541 gctcggcatt ggtagttctc ttagtagcca cgatgtcatc accctgatcc cggtcctggg
     6601 caaccccgtg ttgcaggcta tctttacctg gatgagtaag attggctcgt ttgcttttag
     6661 tttcctgcca gtcatgttct gtatcgccat cccgctgggt ctggcacgcg aaaacaaagg
     6721 cgtagcggca ttcgctggct tcgtcggtta tgcggtaatg aacctcgcgg taaacttctg
     6781 gttgaccaat aaaggcattc tgccaaccac ggacgccgcg gttctgaaag ccaataacat
     6841 ccagagcatt cttgggatcc agtcgatcga caccggtatc ctcggtgcgg tgatcgccgg
     6901 tattatcgtc tggatgctgc atgagcgatt ccataatatc cgcctgccgg atgcgcttgc
     6961 attcttcggc ggtacgcgct tcgtaccaat catctcctcg ctggtgatgg gccttgtcgg
     7021 cctggtgatt ccattagtct ggccgatttt cgcgatgggt attagcggct tgggccatat
     7081 gataaacagc gcgggtgatt tcggaccgat gctgtttggt accggtgaac gtctgctgtt
     7141 gccgtttggt ctgcatcaca ttctggtggc attaattcgc tttaccgacg caggcggcac
     7201 gcaggaagtc tgcggtcaaa ccgtcagcgg cgcattgacc atcttccagg cgcaattgag
     7261 ttgcccgacc actcacggtt tttctgaaag cgccacgcgt ttcctttcgc aaggtaaaat
     7321 gcctgcgttt ctcggcggtc tgccaggtgc agcgttagct atgtatcact gcgcgcgccc
     7381 ggaaaatcgc cataaaatta aaggtctgct gatttctggc ctgatcgcct gcgtcgttgg
     7441 cggcactacc gaaccgctgg aattcctgtt cctgttcgta gcgccagttc tgtatgtcat
     7501 ccacgcgctg ttaaccggcc tcggcttcac cgtcatgtct gtgctcggcg tcaccatcgg
     7561 taataccgac ggcaatatca tcgacttcgt ggtgttcggt attttgcatg gtctgtcaac
     7621 caagtggtac atggtgccag tggtggcggc aatctggttt gtcgtttact acgtcatctt
     7681 ccgtttcgct atcacccgct tcaatctgaa aaccccgggg cgcgatagcg aagttgccag
     7741 ctcaatcgaa aaagccgttg ccggtgcgcc gggtaaatca ggttacaacg ttcctgcaat
     7801 cctcgaagca ttaggcggtg ccgacaatat tgtcagcctc gataactgca ttacccgtct
     7861 gcgtttgtct gtgaaagata tgtcgcttgt taatgtgctg gcactgaagg acaatcgggc
     7921 aattggcgta gtacaactta atcaacataa cctgcaggtt gttatcgggc cacaagttca
     7981 gtcagtaaaa gatgaaatgg ccggtctgat gcatactgtc caggcataag gataagatat
     8041 gttcgatttt tcaaaggtcg tggatcgtca tggcacatgg tgtacacagt gggattatgt
     8101 cgctgaccgt ttcggcactg ctgacctgtt accgttcacg atttcagaca tggattttgc
     8161 cactgccccc tgcattatcg aggcgctgaa tcagcgcctg atgcacggcg tatttggcta
     8221 cagccgctgg aaaaacgatg agtttctcgc ggctattgcc cactggtttt ccacccagca
     8281 ttacaccgcc atcgattctc agacggtggt gtatggccct tctgtcatct atatggtttc
     8341 agaactgatt cgtcagtggt ctgaaacagg tgaaggcgtg gtgatccaca cacccgccta
     8401 tgacgcattt tacaaggcca ttgaaggtaa ccagcgcaca gtaatgcccg ttgctttaga
     8461 gaagcaggct gatggttggt tttgcgatat gggcaagttg gaagccgtgt tggcgaaacc
     8521 agaatgtaaa attatgctcc tgtgtagccc acagaatcct accgggaaag tgtggacgtg
     8581 cgatgagctg gagatcatgg ctgacctatg cgagcgtcat ggtgtgcggg ttatttccga
     8641 tgaaatccat atggatatgg tctggggcga gcagccgcat attccctgga gtaatgtggc
     8701 gcggggagac tgggcgttgc tgacgtcggg ctcgaaaagt ttcaatattc ccgcgctgac
     8761 cggtgcttac gggattatag aaaatagcag cagccgtgat gcctatttat cggcactgaa
     8821 aggacgtgat gggctttctt ccccttcggt actggcgtta actgcccata tcgccgccta
     8881 tcagcaaggt gcaccgtggc tggatgcctt acgcgtctat ctgaaagata acctgacgta
     8941 tatctcagat aaaatgaacg ccgcgtttcc tgaactcaac tggcagatcc cgcaatccac
     9001 ttatctggcc tggcttgatc tacgtctgtt gaatattgac gacaacgcgt tgcaaaaagc
     9061 gcttatcgag caagaaaaag tcgcgatcat gccggggtat acctatggtg aagaaggtcg
     9121 tggttttgtc cgtctcaatg ccggctgccc acgttcgaaa ctggaaaaag gtgtggctgg
     9181 attaataaac gccatccgcg ctgttcgcta accccaattg cgcaacgtaa aaaatcgttg
     9241 cgcaatcgtg gatttttacc ctgctttgtt tttataatgg tgcgcacttt tatattcaga
     9301 aaaagagtgc gaccatgatt gataccaccc tgccattaac tgatatccat cgccaccttg
     9361 atggcaacat tcgtccccag accattcttg aacttggccg ccagtataat atctcgcttc
     9421 ctgcacaatc cctggaaaca ctgattcccc acgttcaggt cattgccaac gaacccgatc
     9481 tggtgagctt tctgaccaaa cttgactggg gcgttaaagt tctcgcctct cttgatgcct
     9541 gtcgccgcgt ggcatttgaa aacattgaag atgcagcccg tcacggcctg cactatgtcg
     9601 agctgcgttt ttcaccaggc tacatggcaa tggcacatca gctgcctgta gcgggtgttg
     9661 tcgaagcggt gatcgatggc gtacgtgaag gttgccgcac ctttggtgtg caggcgaagc
     9721 ttatcggcat tatgagccgg accttcggcg aagccgcctg tcagcaagag ctggaggcct
     9781 ttttagccca ccgtgaccag attaccgcac ttgatttagc cggtgatgaa cttggtttcc
     9841 cgggaagtct gttcctttct cacttcaacc gcgcgcgtga tgcgggctgg catattaccg
     9901 tccatgcagg cgaagctgcc gggccggaaa gcatctggca ggcgattcgt gaactgggtg
     9961 cggagcgtat tggacatggc gtaaaagcca ttgaagatcg ggcgctgatg gattttctcg
    10021 ccgagcaaca aattggtatt gaatcctgtc tgacctccaa tattcagacc agcaccgtag
    10081 cagagctggc tgcacatccg ctgaaaacgt tccttgagca tggcattcgt gccagcatta
    10141 acactgacga tcccggcgta cagggagtgg atatcattca cgaatatacc gttgccgcgc
    10201 cagctgctgg gttatcccgc gagcaaatcc gccaggcaca gattaatggt ctggaaatgg
    10261 ctttcctcag cgctgaggaa aaacgcgcac tgcgagaaaa agtcgccgcg aagtaacaaa
    10321 atggatggtg caaattgcac catccatttt tcatgcaagg cacaaagtcg cgcgatgttt
    10381 ggcggattcg atgcccagct caatcaactc cattacctgg attgcctggc ttgccggaac
    10441 cggattttca ccatcgccat ttaacgcatc acgaatagcc gcatagtaag ccggataatt
    10501 cccaggtacc gtcaacagcg tttcttcgac acgttcctca ccttccacgc gggtaagtac
    10561 gccatcacgc atatcgtagc cccagtcttc ctgcggcaga cgctcgccat ttttcagacg
    10621 ttcttcctgt ggatcgaggc catatttcac ataactgcct cgggatccat gcacgatata
    10681 ccgtgctgac tcagcggctg ccagcatggt accgtgtaaa atgactcgcc gctgtggata
    10741 ggacaagatg gcgtggaaat aatcggtcga ctgcgctccg ggccgtaact gtgccaaatc
    10801 taccgtcatg ctgaccggta aaccaaaaag cgtaatggcc tgatcaagaa gatgtggtgc
    10861 taaatcgtac cagataccgc tgcctgggcc gccctgttca cgccaacgat cgcgcacctg
    10921 cggacggaag cggtcaaaat gagactcaaa gtaagcaact tcacccagca cgccttccgc
    10981 gagtaaacct tttagcgtca agaaatcgct atcccagcga cggttatgga atacagacag
    11041 cacacgcccc aggctttttg ccagcgcatc cagctctcgc gcttgtgaca gtgtcacggt
    11101 aaagggttta tcaacgacca catgtttacc cgcctcaagc gccgctttgg ctaacgggaa
    11161 atgggtatcg ttgggtgtag gaatgacaat caggtctatg ttgggatcgt taaacagatg
    11221 cttcggctca gagacaaccg taaccgttgg ccagtcggct tttacttttg tttcatcact
    11281 gctggagatt accgccagtt ccagcccggg cgtgcccgca atcaggggcg catggaaggt
    11341 tttgctcgca taaccatacc caatcaaccc aacacggatg ttgtcgctca tgtcattgcc
    11401 tctcattgaa gtatgatggc tatttgacac tatcctttac ccacgctcaa cagtttaata
    11461 acctgccagc aataagggat gttgtttaac ttaagtcaaa aaaatagcga attttccaac
    11521 gacaaaagct aaatatcgca aaaacctcag taaaaatctt gctggagcta ttattgctaa
    11581 gtaacattta ccccctgaag ttaatggatc aatcaagaga gatgtgggct gtaatgaatc
    11641 gtcttattga attaacaggt tggatcgttc ttgtcgtttc agtcattctt cttggcgtgg
    11701 cgagtcacat tgacaactat cagccacctg aacagagtgc ttcggtacaa cacaagtaag
    11761 ctctgcactt gtggagcgac atgctgcccg tccgggtgca tgttttcact tgtcggatat
    11821 taaaccagga atttattatc ttgttcgatg ttgttggtga ttgtcaggga tagtaaagcg
    11881 cgttactctt ttcagagcag tttttcatcc ctgaatattt gccatcagcg cattcttatt
    11941 attgtaataa cacaagcgtt cacttttggt gaaattttat catcactaat aatacctacc
    12001 cctttggtat taattatttt atggaccttt tatgactgtt caggactact tattaaaatt
    12061 tcgcaaaatc agttcactcg aaagtctgga aaaactctac gaccatctta attacaccct
    12121 gacggacgat caggaactga tcaatatgta tcgtgctgcc gatcaccgtc gcgcagagct
    12181 ggtttctggc gggcgtttgt ttgacctcgg ccaggtaccg aagtccgtct ggcactatgt
    12241 ccaataaaga aagtagcgat cggttataaa agccttataa taacgcccct aaaattagat
    12301 ttgcctgttt gcgtcgctat tctggagaac ctatgaccac cacaacgcca caacgtattg
    12361 gaggctggtt gcttggcccg ttggcctggc ttttagtcgc tctgttgagt acgacgctgg
    12421 cgttgttgct gtataccgct gcgttatctt ctccgcaaac atttcaaacg ctcggcggac
    12481 aagcactgac gacacaaatc ttatggggcg tttcttttat taccgctatc gccatgtggt
    12541 attacacact gtggttgacc atcgctttct ttaaacgtcg gcgttgtgtt cctaaacact
    12601 atattatctg gctgcttatt tccgtattgc tggcagtaaa agcattcgct ttttcacccg
    12661 tggaagatgg catagccgtt cgccagttgc tgtttacttt gttggcaacc gcattgatcg
    12721 tcccctattt caaacgctcg tcgcgggtaa aagcgacgtt tgtgaatccg taataacctt
    12781 acagttaacc tgttgtcgcc tgctctggat taacggataa taggcggctt ttttatttca
    12841 ggccgaaaaa tgactgacta cctgttactg tttgtcggaa ctgtactggt caataacttt
    12901 gtactggtca agtttctcgg tctctgtccg tttatggggg tttccaaaaa actggaaacc
    12961 gcgatgggca tggggctggc aacaacgttt gtgatgacgc tggcgtctat ttgcgcctgg
    13021 cttatcgata cgtggatttt gatcccactt aatctgattt acctgcgcac cctggcattt
    13081 attctggtga ttgctgtggt cgtgcagttc accgagatgg tggtgcgcaa aaccagcccg
    13141 gtgctttacc gtctgctggg gatttttttg ccgcttatca ccaccaactg tgcagtgctc
    13201 ggcgtggcgt tgctgaatat caatctcggg cacaatttct tgcagtcggc gctgtacggt
    13261 ttttccgccg ctatcggttt ctcgctggtg atggtgctct tcgccgccat ccgcgaacgc
    13321 cttgctgtgg ctgatgtccc ggcacctttt cgcggtcatg ccattgcgtt aattaccgca
    13381 ggtcttatgt ctctggcctt tatgggcttt agtggtttgg tgaagttgta atgaatgcta
    13441 tctggattgc cgttgccgcc gtgagcctgc tgggcctggc gtttggcgcc attctgggtt
    13501 atgcctcccg ccgttttgcg gtggaagacg atccggtcgt tgagaaaatt gacgaaatct
    13561 taccgcagag ccagtgtggt cagtgcggtt atcccggctg tcgcccctac gcggaagcca
    13621 tcagctgtaa cggtgaaaaa atcaaccgtt gcgccccagg tggcgaagct gtgatgctaa
    13681 aaattgccga gttgcttaat gtcgagccgc agccgctgga tggcgaagcg caagagataa
    13741 cgcctgcgcg gatggtggcg gttattgatg aaaataactg tattggctgc actaaatgta
    13801 ttcaggcgtg tccggtagac gccatcgttg gcgctacccg tgccatgcat acggtaatga
    13861 gtgatctctg tacgggctgc aatttatgtg ttgatccgtg cccgacgcac tgcatctcgt
    13921 tgcaaccggt cgcagaaaca cctgactcct ggaaatggga tctgaacacc attcccgtgc
    13981 gtatcattcc cgtggaacac catgcttaag ttattctctg cattcagaaa aaataaaatc
    14041 tgggatttca acggcggcat ccatccaccg gagatgaaaa cccagtccaa cggtacaccc
    14101 ctgcgccagg taccactggc gcagcgtttt gttattccac tgaaacagca tattggcgct
    14161 gaaggtgagt tgtgcgttag cgtcggcgat aaagtattgc gcggccagcc gcttacccgt
    14221 ggtcgcggca aaatgctgcc tgttcacgcg cccacctcgg gtaccgttac ggctattgcg
    14281 ccccactcta cggctcatcc ttcagcttta gctgaattaa gcgtgattat tgatgccgat
    14341 ggtgaagact gctggatccc gcgcgacggc tgggccgatt atcgcactcg cagtcgcgaa
    14401 gagttaatcg agcgcataca tcagtttggt gttgccgggc tgggcggtgc aggattcccg
    14461 acaggcgtta aattgcaggg tggcggagat aagattgaaa cgttgattat caacgcggct
    14521 gagtgcgagc cgtacattac cgccgatgac cgtttgatgc aggattgcgc ggctcaggtc
    14581 gtagagggta ttcgcattct tgcgcatatt ctgcagccac gcgaaattct tatcggcatt
    14641 gaagataaca aaccgcaggc gatttccatg ctgcgcgcgg tgctggcgga ctctaacgat
    14701 atttctctgc gggtgattcc aaccaaatat ccttctggcg gtgctaaaca attaacctac
    14761 attctgaccg ggaagcaggt tccacatggc gggcgttcat ccgatatcgg cgtattaatg
    14821 caaaacgtcg gcactgctta tgcagtgaaa cgtgccgtta ttgatggcga gccgattacc
    14881 gagcgtgttg taaccctgac tggcgaagca atcgctcgcc cgggcaacgt ctgggcacgg
    14941 ctggggacgc cagtgcgtca tttattgaat gatgccggat tctgcccctc tgccgatcaa
    15001 atggtgatta tgggtggccc gctaatgggc tttaccttgc catggctgga tgtcccggtc
    15061 gtaaagatta ccaactgtct gttggctccc tctgccaatg aacttggcga accacaggaa
    15121 gaacaaagct gcatccggtg tagcgcctgt gctgacgcct gccctgcgga tcttttgccg
    15181 caacagttgt actggttcag caaaggtcag caacacgata aagctaccac gcataacatt
    15241 gctgattgca ttgaatgtgg ggcttgcgcg tgggtttgcc cgagcaatat tcccctggtg
    15301 caatatttcc gtcaggaaaa agctgaaatt gcggctattc gtcaggaaga aaagcgcgcc
    15361 gcagaagcca aagcgcgttt cgaagcgcgc caggctcgtc tggagcgcga aaaagcggct
    15421 cgccttgaac gacataagag cgcagccgtt caacctgcag ccaaagataa agatgcgatt
    15481 gctgccgctc tggcgcgggt gaaagagaaa caggcccagg ctacacagcc tattgtgatt
    15541 aaagcgggcg aacgcccgga taacagtgca attattgcag cacgggaagc ccgtaaagcg
    15601 caagccagag cgaaacaggc agaactgcag caaactaacg acgcagcaac cgttgctgat
    15661 ccacgtaaaa ctgccgttga agcagctatc gcccg
//
LOCUS       DAZGLJ010000106        15691 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000106 DAZGLJ010000000
VERSION     DAZGLJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.106"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            98..340
                     /gene="yjbE"
                     /locus_tag="ACHK49_002869"
     CDS             98..340
                     /gene="yjbE"
                     /locus_tag="ACHK49_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO9263487.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            454..1092
                     /locus_tag="ACHK49_002870"
     CDS             454..1092
                     /locus_tag="ACHK49_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO9263488.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1089..1826
                     /locus_tag="ACHK49_002871"
     CDS             1089..1826
                     /locus_tag="ACHK49_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO9263489.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1826..3922
                     /locus_tag="ACHK49_002872"
     CDS             1826..3922
                     /locus_tag="ACHK49_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO9263490.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKVAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3969..4247)
                     /locus_tag="ACHK49_002873"
     CDS             complement(3969..4247)
                     /locus_tag="ACHK49_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263491.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4248..4391
                     /locus_tag="ACHK49_002874"
     CDS             4248..4391
                     /locus_tag="ACHK49_002874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263492.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4517..4927
                     /gene="psiE"
                     /locus_tag="ACHK49_002875"
     CDS             4517..4927
                     /gene="psiE"
                     /locus_tag="ACHK49_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO9263493.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4971..6446)
                     /gene="xylE"
                     /locus_tag="ACHK49_002876"
     CDS             complement(4971..6446)
                     /gene="xylE"
                     /locus_tag="ACHK49_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO9263494.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLHTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDATWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNALAAQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVTHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6818..7708)
                     /gene="malG"
                     /locus_tag="ACHK49_002877"
     CDS             complement(6818..7708)
                     /gene="malG"
                     /locus_tag="ACHK49_002877"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO9263495.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7723..9267)
                     /gene="malF"
                     /locus_tag="ACHK49_002878"
     CDS             complement(7723..9267)
                     /gene="malF"
                     /locus_tag="ACHK49_002878"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO9263496.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTTQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9421..10611)
                     /gene="malE"
                     /locus_tag="ACHK49_002879"
     CDS             complement(9421..10611)
                     /gene="malE"
                     /locus_tag="ACHK49_002879"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO9263497.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10976..12091
                     /gene="malK"
                     /locus_tag="ACHK49_002880"
     CDS             10976..12091
                     /gene="malK"
                     /locus_tag="ACHK49_002880"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO9263498.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12163..13503
                     /gene="lamB"
                     /locus_tag="ACHK49_002881"
     CDS             12163..13503
                     /gene="lamB"
                     /locus_tag="ACHK49_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO9263499.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13746..14666
                     /gene="malM"
                     /locus_tag="ACHK49_002882"
     CDS             13746..14666
                     /gene="malM"
                     /locus_tag="ACHK49_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO9263500.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14895..15691
                     /locus_tag="ACHK49_002883"
                     /pseudo
     CDS             14895..15691
                     /locus_tag="ACHK49_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT         3953 a         3956 c         4073 g         3709 t
ORIGIN      
        1 ccccgcgccg taattcgcat gctttagttg tgtatactcg atcccgcccg aaatgttttt
       61 gggtaaatct ccattcattc aatgaaggga aattgttatg aaaaaagttc tgtatggcat
      121 ttttgccata tctgcgcttg cggcgacttc tgcgtgggct gcacctgtac aggtgggcga
      181 agcggcaggg tcggcagcaa cgtcggtttc ggcggggagt tcctccgcta ccagcgtcag
      241 caccgtaagc tcggcggtgg gtgtcgcgct cgcggcaacc ggtggcggtg atggttctaa
      301 taccgggacc accacaacca cgaccaccag tacccagtaa taaagtatgt atccccaaaa
      361 taatttgagt cattgcatct gtggcttgaa gtatgaaggg atttaccata accacactcc
      421 ggtgtggtta ttctgcccct ctggagaaga gtcgtgaagc gacctgcact cattcttatc
      481 tgcctgctat tacaggcctg ttcagccacg actaaagagc tgggcaattc actgtgggac
      541 agtctgttcg gcacgccagg cgtacagctg acggacgatg atattcaaaa tatgccctac
      601 gccagccagt acatgcagct taatggcggg ccgcaattgt ttgtggtgct ggccttcgct
      661 gaagacggac aacaaaaatg ggtcactcag gatcaagcca ctcttgtcac acaacatggt
      721 cgtctggtga agactttgct tggcggcgac aacctgattg aagtgaataa ccttgccgcc
      781 gacccgctga ttaaacccgc acaaattgtt gatggtgcaa catggacgcg cacgatgggc
      841 tggaccgagt accagcaggt acgctacgcc accgcacgct cagtcttcaa atgggatggc
      901 accgataccg tcaaagtcgg cagcgatgaa accctggttc gcgtgctgga cgaagaagtc
      961 tccaccgacc aggcgcgctg gcataaccgc tactggatcg acagcgaagg gcaaattcgc
     1021 cagtcggaac agtatctcgg cgcggattat ttcccggtga aaaccactct catcaaggcg
     1081 gcaaaacaat gattaaacaa acttttgtcg cgttgctttt gagtgtggga gcgtcatcgg
     1141 tcttcgcggc aggaaccgtc aaggtgttca gcaatggcag cagtgaggcc aaaacactaa
     1201 caggcgcaga gcatttaatc gatctggtag gccaaccgcg gctggcaaac agctggtggc
     1261 ccggtgcggt gattagcgaa gagctggcaa cggcggcagc attgcgtcag cagcaggcgt
     1321 tgctgacacg gctggcagaa cagggcgcag attccagcac cgacgatgcc gccgccatta
     1381 acgccttacg ccagcaaatc caggcattga aagtgacagg caggcaaaaa atcaatcttg
     1441 atcccgatat cgtccgcgtt gccgaacgcg gtaacccgcc gttgcagggc aactacacgc
     1501 tgtgggtcgg accaccgccg tccacggtca cgttgttcgg gcttatcagc catcctggca
     1561 atcagccatt cactccaggt cgcgacgtgg cgagctatct ctccgggcag aacctgctta
     1621 gcggtgcgga tcgcagctac gcgtgggtcg tttacccgga cggacgcacg caaaaagcgc
     1681 cggtggctta ctggaacaag cgtcacgtag agccgatgcc cggcagcatt atttatgttg
     1741 gcctcgcgga ctccgtctgg agtgagacgc ctgatgccct taacgccgac attcttcaga
     1801 ctctgacgca gcggataccc caataatgaa aaaaagacat ctgcttagct tactggcgct
     1861 gggcattagc atggcttgct acggcgaaac atatcctgcg cccattggcc cgtcgcagtc
     1921 ggatttcggt ggcgtaggat tattacaaac tcccaccgcg cgtatggcac gggaagggga
     1981 gttgagtctg aactatcgcg ataacgatca gtaccgttat tactcagctt cagtgcaact
     2041 cttcccgtgg ctggaaacaa cgctgcgcta caccgacgtg cgcacccggc agtacagcag
     2101 cgtcgaagcg ttctctggcg atcaaacgta taaagataaa gccttcgatc tcaaactgcg
     2161 tttgtgggaa gagagttatt ggctgccgca agtggcggtt ggtgcgcggg atatcggcgg
     2221 tacggggctg tttgatgcgg aatatcttgt tgccagcaaa gcctgggggc cgttcgattt
     2281 tacgctcggc ctgggctggg gatatctggg caccagcggt aatgtgaaaa atccgctctg
     2341 ttcagccagt gataaatatt gctatcgcga taacagctac aaacaggcgg gatctatcga
     2401 cggtagccag atgttccacg gtcctgcctc actgtttggc ggcgtggaat accagacgcc
     2461 ctggcaaccg ctgcgcctga aactggagta tgaaggcaat aattatcagc aggattttgc
     2521 cgggaagctg gagcaaaaaa gtaagtttaa cgtcggtgcg atttatcgcg ttaccgattg
     2581 ggccgacgtt aaccttagct atgaacgtgg caacaccttt atgtttggcg ttacgttgcg
     2641 caccaacttt aacgatctgc gcccgtctta caacgataac gcccgcccgc aatatcaacc
     2701 gcagccgcag gatgccattt tgcagcattc ggtggtggcg aatcagttaa cgttgctgaa
     2761 atacaatgcc ggacttgccg atccgcagat ccaggcgaaa ggcgatacgc tgtatgttac
     2821 cggcgagcag gtgaaatatc gtgattcgcg cgaagggatc atccgtgcga atcggatcgt
     2881 gatgaacgat ctgccggatg ggatcaaaac gatccgcatt acagaaaatc gccttaacat
     2941 gccgcaggtg acgacggaaa ccgatgtcgc cagcttgaaa aatcatctcg ccggggagcc
     3001 gctgggccat gaaacgaagg tggcgcaaaa acgcgtcgag ccagtggttc cgaagtccac
     3061 cgagcaggga tggtatatcg acaaatcacg ctttgatttc catatcgatc cggtgctgaa
     3121 ccagtcggtc ggtggcccgg aaaactttta catgtatcag ctgggcgtga tgggaacggc
     3181 agatttgtgg ctgacggacc atctgctgac caccggcagc ctgtttgcaa atcttgccaa
     3241 caactacgac aagtttaact acaccaatcc gccgcaggac tcgcacttac cgcgcgttcg
     3301 tacccatgtg cgcgagtatg tgcagaacga tgtctatgtg aataacctgc aagccaacta
     3361 cttccagcat ctgggcaacg gcttctacgg tcaggtctat ggaggttatc tcgaaaccat
     3421 gtttggcggt gccggggcag aagtgttgta tcgtccgctg gacagcaact gggcgtttgg
     3481 tctggatgcc aactacgtta aacagcgcga ctggcgtagt gcaaaagata tgatgaaatt
     3541 caccgactac agcgtgaaaa ccggacatct gaccgcctac tggacgccat ctttcgccca
     3601 ggacgtgttg gtgaaagcga gcgtcgggca gtatctggca ggggataaag gcggcacgct
     3661 ggagatcgcc aaacgctttg atagcggcgt ggtggttggc ggctatgcca cgatcacgaa
     3721 tgtttcgaaa gaggagtacg gcgaagggga cttcaccaaa ggcgtgtatg tctctgtgcc
     3781 gttggatctc ttctcgtctg gcccgacacg cagccgtgcg gcgattggct ggacgccgct
     3841 gacgcgtgac ggtggtcagc aacttgggcg taagttccag ttgtatgaca tgaccagcga
     3901 ccgtagcgtc aatttccgct aagtcatggg aaaggtgcca gttttcgcaa tcactggcac
     3961 cctcattctt aatggcctgt tgagccatca ggaacgcagt tcacccccgg tttgcgatct
     4021 ggtgtgtaaa ggcgaaaaca ttctgtcttc ctgtccgtcg atatcggttg cagatctggt
     4081 tgcctgagcg actgggccat cattgggctg gagacattgc tttgcggtag gtcggcttta
     4141 tcagcagtac cgagcggacc agcataagca ggaagaacag agactgataa catcaaagca
     4201 gcaaaataag gcttcatttt taccaccttt atcaggttac gtttcatttg ttccagagga
     4261 acattgtcga tttttcgcgc attgctggtg gctgggaatc acctgaatgg gtgatttttg
     4321 aattaccggc tttggtgcgg tttgtcttgc cggatgcgcc gccaggcgcg gcttatccgg
     4381 cctacgggta ggtatatccg gctttggtgg aggcgcgctc caaatccagg ttgaacaaaa
     4441 catacacaaa aaatatagat ctccgtcaca tttttgcgtt atacaggaag ctcgccactg
     4501 tgaaggaggt actgctatga cgtcactctc tcgtccgcgc gtggagttta tctccaccat
     4561 tttgcagacc gtactcaatc ttggcctgtt gtgcctcggc ctgattttgg ttgtcttcct
     4621 cggcaaagaa acggtgcatc tggctgatgt gctgttcgcg ccagaacaaa ccagcaaata
     4681 tgagctggta gaagggctgg tggtttactt tctctatttc gaatttatcg cgctgattgt
     4741 gaagtacttt cagtccggtt ttcacttccc gctacgttac tttgtctata ttgggatcac
     4801 cgccattgtg cgcttgatca tcgtcgatca taaatcgcca ctggatgtat tgatctactc
     4861 ggcagcgatc ctgttactgg tgatcaccct gtggctgtgt aattcgaaac ggctgaagcg
     4921 ggagtaaaaa gacagcacgc cgaactggcg cggcgtgctg gacaggaaga ttacagtgta
     4981 gcagtttgtt gtgttttctt cgtttccggt tcccagaggg cttccagctc ctcaagggtt
     5041 ttacctttgg tttccgggac aaatttccac ataaatagtg ctgccagaac gcccatacaa
     5101 ccgtaaatcc agtaggagaa accgttgtgg aaatgggtca ccagccagga gtttttgtcc
     5161 atcatcggga aggtccagga gacgaagtag ttcgccagcc actgggccgc caccgcgatt
     5221 gccagcgctt taccacgaat agcattcggg aagatttccg acagcagtac ccagcatacc
     5281 ggaccccagg acatggcaaa ggcagcaaca tagaacagca tcgacagtag cgccacaata
     5341 cccggtgcct gagtgtaaaa cgcggtaccg aggctaaaca taccgattgc cattccgagc
     5401 gcgccgataa tttgcagtgg cttacgacca aatttatcca ccgtcataat tgccagaacg
     5461 gtgaaggtga ggttgataac tccgacaata atggtctgca acagcgcgat atccgtgctg
     5521 gcccccagcg ttttgaacac ttccggcgcg tagtacaaca ccacattgat gccgacaaat
     5581 tgctggaaga tggagagcat aacgccgata acaatcacgc ccacgccgaa catcagcaga
     5641 cgaccaccgg ttttgcgtcc atgatccagg gagtgtttaa tttcctgcac cgcctgggct
     5701 gccaaagcgt tgcccataat tttgcgcagg atgccttccg cttgttcttg cttaccgcgt
     5761 gacatcagcc agcgagggct ttccggcacg gtataaagca gcattaagaa cagcagcgca
     5821 gggatacatt ccgaagcaaa catataacgc cagccgtcag tattcagcca ggtggcatcg
     5881 ccggaacggg caataaaata gtttacgcag taaactaaaa gttggccgaa aataatcgca
     5941 aactggttaa aagagaccag cttcccacga atatgtgcag gagccagttc cgcaatatac
     6001 attggtgaga gcattgaggc taaaccaaca ccaataccgc caataatgcg ataaataaca
     6061 aattccggga cataacctgc cagataaaca ggcactgtgt tgtccgggtt tatagaggta
     6121 aaaccaagtt ctggccaggc agaacctaca ccagaaataa aaaacaggac agcagcaatc
     6181 ttaagtgaat cacgacgacc gaagcggtta ctgcaataac caccgagggc accgccgatg
     6241 atgcaaccaa tcagagcgct ggctacgcaa aaccctaaca gggagttggc agcggattca
     6301 cttaagtttt gtggagcaac aaagacggta tggagtgact caacagtacc ggaaataacg
     6361 gcggtgtcgt agccaaataa taaaccacct aatgtagcga ctaaggtaat cgaaaatata
     6421 taactggaat tatactgggt attcattcag acctgcctta gaccattctg atcttttatc
     6481 agtgcattga tgttggaatt gtcaggcagt taaggcaatt atcatttttt acaatgcgtt
     6541 tacgtaatgt cttttctgtg atcttaattg tgataattat ctaaaattga tgaaaacaat
     6601 actattgccg tgactcagag cacgaaagag aattatcgta agtgggaaaa caaataacgt
     6661 aaaaataata agctctatga tgggaatatt aaccggcgaa cgattcagat tgcagacgaa
     6721 agaaaaaaag gcgctccgtg gagcgccgaa taacagtcac aagttgggat aacgtaagtt
     6781 gagggtgcag cggcataaca ttggcagaac aacatcttta acctttcaca ccacctgccg
     6841 tcaggccgtt gaccagccag cgttgagcca gcaagaagac gatggtgatc ggtaatgcag
     6901 acatcacggc agcggcggca aagtcacccc acaggtagtt ttgcgggttg aggtattgct
     6961 gcatccccac ggccagggtg tagctgttta cgtcacgcag taacagcgac gcgaccggaa
     7021 cttcagtaat ggcagcgata aacgacaaga tgaataccac cgccagaatc ggtactgaca
     7081 gcggcaacag gacaaggcgg aaggcctgcc acggtgtcgc accatccagc gcagccgctt
     7141 cttccagcga actatcgatg gtttcgaaat agcctttgat ggtccagaca tgcagcgcaa
     7201 tcccacccag atacgcgaaa attacgccgc cgtgagtatt caggccaatg aatggaatgt
     7261 actcacccag acgatcaaac aacgcataca acgcgaccag tgaaagtact gccgggaaca
     7321 tctggaaaat cagcattcct ttcagcagcg tcgctttgcc tggaaagcgc atacgggcga
     7381 aagcgtaggc gcaggtggta gagagcgcca caatgccaat cgcggaaatc ccggcgacct
     7441 ttaccgagtt ccacagccac agcagtaccg ggaatggcgg tggcgtaatg cgaccatcag
     7501 cctgttcaac gctaaaacct aacgccagtt tccagtgatc ccaggagatt tgctccggga
     7561 tcaggctacc ggtcgcgaag tttccctgac gcagcgagat agcgacgacc atcagcagcg
     7621 ggaacataat cgccgcgata aaaagtagca gtagcaggtg agtaataaac aaacgtgctt
     7681 tttgcgattt cggttggacc attgccattt ttgttatctc ccttaatcaa acttcattcg
     7741 cgtggctttc aggttcacta tcgccagcgc acccaccagc aggaagatca gcgtggcaat
     7801 tgctgctgcc agaccgaagt cctgaccccc gccgccttca aaagcgatgc ggtaggtgta
     7861 gttaacaagc aggtcggtat aaccggctgg cgtggtcgtg ccaagacgat ccgggccgcc
     7921 gttggttaac agttgaatca gcacgaagtt gttaaagtta aaggcgaagc tggcgatcat
     7981 cagcggcgtc agcggtttaa tcagcagcgg cagcgtaatc ttaaagaagt tctggaacgg
     8041 acctgcgcca tccattgctg aggcttcata caaatcgtcc ggaatcgctt tcagcaagcc
     8101 catgcagagg atcatcatgt acggataacc cagccaggta ttgacgataa tcagcatcgt
     8161 gcgggcggtg gtcggatcgc tgaaccaggc gggcttcacg ccaaacagcg cgctcaacat
     8221 catgttgatt tcaccgaagc tctggttaaa caaccctttg aaaatcaaga ttgaaatgaa
     8281 cgatggcacc gcgtagggca gaatcagcag gacgcgatag accgctttgc cgcgcaacgc
     8341 ttcccactgc accagacacg ccagaaccat gccgaccgcc accgttaaaa agacagtgat
     8401 cagcgagaac accacggtcc agacgaaaat ggcgaggaac ggtttctgaa tgccttcgtc
     8461 ggtgaagacg cgggtaaagt ttttccagcc agtagtcacg gtgtaaccgg ggcttagctt
     8521 ttcatcgccc cagttgccgt cggcggtaat ggactggtaa aagccaattt ggttattcgg
     8581 acgatatttc acgccgctct gattattcgt caacgtgccg tcaccgtcga gtgtgtagag
     8641 cggctgcgtg ccagaaaact ggcgcaggga gctcatcatc actttgttgc catccggcag
     8701 aatggcggta atgtcactca gcgcctgacg attctgggta atcacgcgca gattcgcgcg
     8761 ttcgccttcg ggctgggtgg tcgtttcttt cagttgcagt ttttgctcgc cgccaaattt
     8821 aaaagcgtcg gagaggtaat ttttgccggt ttcgccgtcg ctgagggcta gttgccactc
     8881 atcgcccgcc gggtaaagac caaaattata ggttttgcct gcttgccagg agcgatctag
     8941 caacacttcc tgcgcacgtt caaaagtcag ctggttagtg ctgctgtagt tggtgaaggc
     9001 aatggcgatg gtgcagacca gagggaagag gacgaataat cccattccag ccattcccgg
     9061 gtaaacatag cgccaggcgt aggctttacg attggcgaaa atatacagcc ccgctgaact
     9121 caatatcagc gtggtaatgg cgaacaggta ttccccttgt gcgtacatta aaacaacaag
     9181 gtaacccacc agcaggccga gcagacctaa cactgaccat ttcagcgcgt cgctttgcca
     9241 ccaatgtttc tttttaatga catccatggg gttcttcctc attccaggac ggataaggct
     9301 ttcacgcctt atccgacaac aactgcctga tgcgacgctg acgcgtctta tcaggcctac
     9361 atacgtttcg gttttgtagg ccggacaagg cgttcacgcc gcatccggca tttcacagca
     9421 ttacttggtg atacgagtct gcgcgtcttt cagggcttca tcgacagtct gacgaccgct
     9481 ggcggcgttg atcaccgcag tacgcacggc ataccagaaa gcggacatct gcgggatgtt
     9541 cggcatgatt tcacctttct gggcgttttc catggtggct gcaatacgtg gatctttcgc
     9601 caactcttcc tcgtaagact tcagcgctac ggcacccagc ggtttgtctt tattaaccgc
     9661 ttccagacct tcgtcagtca gcagatagtt ttcgaggaac tctttcgcca gctctttgtt
     9721 cggactggcg gcgttaatac cggcgctcag cacgccaacg aacggtttag atggttgacc
     9781 cttgaaggtc ggcagtaccg ttacaccata attcactttg ctggtgtcga tgttggacca
     9841 tgcccacggg ccgttgatgg tcatcgctgt ttcgccttta ttaaaggcag cttctgcgat
     9901 ggagtaatcg gtgtctgcat tcatgtgttt gtttttaatc aggtcaacca ggaaggtcag
     9961 acccgctttc gcgccagcgt tatccacgcc cacgtcttta atgtcgtatt tgccattttc
    10021 atacttgaac gcataacccc cgtcagcagc aatcagcggc caggtgaagt acggttcttg
    10081 caggttgaac atcagcgcgc tcttaccttt cgctttcagt tctttatcca gcgccgggat
    10141 ctcttcccag gtttttggtg ggttcggcag cagatctttg ttataaatca gcgataacgc
    10201 ttcaacagcg atcgggtaag caatcagctt gccgttgtaa cgtacggcat cccaggtaaa
    10261 cggatacagc ttgtcctgga acgctttgtc cggggtgatt tcagccaaca ggccagattg
    10321 agcgtagcca ccaaaacggt cgtgcgccca gaagataatg tcagggccat cgccagttgc
    10381 cgcaacctga gggaatttct cttccagttt atccggatgc tcaacggtga ctttaattcc
    10441 ggtatctttc tcgaatttct taccgacttc agcgagaccg ttatagcctt tatcgccgtt
    10501 aatccagatt accagtttac cttcttcgat tttggcgaga gccgaggcgg aaaacatcat
    10561 cgtcgttaat gcggataatg cgaggatgcg tgcacctgtt tttattttca taatctatgg
    10621 tccttgttgg tgaagtgctc gtgaaaacac ctaaacggac tctagtttct ttatacggca
    10681 acctctttcc atcctccttg cccctacgcc ccaccgttgc tttgtgtgat ctctgttaca
    10741 gaattggcgg taatgtggag atgcgcacat aaaatcgcca cgatttttgc aagcaacatc
    10801 acgaaattcc ttacatgacc tcggtttagt tcacagaagc cgtgttctca tcctcccgcc
    10861 tcctccccca taaaaaagcc agggggtgga ggatttaagc catctcctga tgacgcatag
    10921 tcagcccatc atgaatgttg ctgtcgatga caggttgtta caaagggaga agggcatggc
    10981 gagcgtacag ctgcaaaatg taacgaaagc ctggggcgag gtcgtggtat cgaaagatat
    11041 caatctcgat atccatgaag gtgaattcgt ggtgtttgtc ggaccgtctg gctgcggtaa
    11101 atcgacttta ctgcgcatga ttgccgggct tgagacgatc accagcggcg acctgttcat
    11161 cggtgagaaa cggatgaatg acactccgcc agcagaacgc ggcgttggta tggtgtttca
    11221 gtcttacgcg ctctatcccc acctgtcagt agcagaaaac atgtcatttg gcctgaaact
    11281 ggctggcgca aaaaaagagg tgattaacca acgcgttaac caggtggcgg aagtgctgca
    11341 actggcgcat ttgctggatc gcaaaccgaa agcgctctct ggcggtcagc gtcagcgtgt
    11401 ggcgattggc cgtacgctgg tggccgagcc aagcgtattt ttgctcgatg aaccgctctc
    11461 caacctcgat gctgcactgc gtgtgcaaat gcgtatcgaa atctcccgtc tgcataaacg
    11521 cctaggccgc acaatgattt acgtcaccca cgatcaggtc gaagcgatga cgctggccga
    11581 caaaatcgtg gtgctggacg caggtcgcgt ggcgcaggtt gggaaaccgc tggagctgta
    11641 ccactatccg gcagaccgtt ttgtcgccgg atttatcggt tcgccaaaga tgaacttcct
    11701 gccggtaaaa gtgaccgcca ccgcaatcga tcaagtgcag gtggagctgc cgatgccaaa
    11761 tcgtcagcaa gtctggctgc cagttgaaag ccgtgatgtc caggttggag ccaatatgtc
    11821 gctgggtatt cgcccggaac atctactgcc gagtgatatc gctgacgtca tccttgaggg
    11881 tgaagttcag gtcgtcgagc aactcggcaa cgaaacccaa atccatatcc agatcccttc
    11941 cattcgtcaa aacctggtgt accgccagaa cgacgtggtg ttggtagaag aaggtgccac
    12001 attcgctatc ggcctgccgc cagagcgttg ccatctgttc cgtgaggatg gcactgcatg
    12061 tcgtcgactg cataaggagc cgggcgttta agcaccccac aaaacacaca aagcctgtca
    12121 caggtgatgt gaaaaaagaa aagcaatgac tcaggagata gaatgatgat tactctgcgc
    12181 aaacttcctc tggcggttgc cgtcgcagcg ggcgtaatgt ctgctcaggc aatggctgtt
    12241 gatttccacg gctatgcacg ttccggtatt ggctggacag gtagcggcgg tgaacaacag
    12301 tgtttccaga ctaccggtgc tcaaagtaaa taccgtcttg gcaacgaatg tgaaacttat
    12361 gctgaattaa aattgggtca ggaagtgtgg aaagagggcg ataagagctt ctatttcgac
    12421 actaacgtgg cctattccgt cgcgcaacag aatgactggg aagctaccga tccggccttc
    12481 cgtgaagcaa acgtgcaggg taaaaacctg atcgaatggc tgccaggttc caccatctgg
    12541 gcaggtaagc gcttctacca acgtcatgac gttcatatga tcgacttcta ctactgggat
    12601 atttctggtc ctggtgccgg tctggaaaac atcgatgttg gcttcggtaa actctctctg
    12661 gcagcaaccc gctcctctga agctggtggt tcttcctctt ttgccagcaa caatatttat
    12721 gactatacca acgaaaccgc gaacgacgtt ttcgatgtgc gtttagcgca gatggaaatc
    12781 aacccgggcg gcacattaga actgggtgtc gactacggtc gtgccaacct gcgtgataac
    12841 tatcgtctgg ttgatggcgc atcgaaagac ggctggttat tcactgctga acatactcag
    12901 agtgtcctga agggctttaa caagtttgtt gttcagtacg ctactgactc gatgacctcg
    12961 cagggtaaag gtctgtcgca gggttctggc gtcgcgtttg ataacgaaaa atttgcctac
    13021 aatatcaaca acaacggtca catgctgcgt atcctcgacc acggtgcgat ctccatgggc
    13081 gacaactggg acatgatgta cgtgggtatg taccaggata tcaactggga taacgacaac
    13141 ggcaccaagt ggtggaccgt tggtattcgc ccgatgtaca agtggacgcc aatcatgagc
    13201 accgtgatgg aaatcggcta cgacaacgtc gaatcccagc gcaccggcga caagaacaat
    13261 cagtacaaaa ttaccctcgc acaacaatgg caggctggcg acagcatctg gtcacgcccg
    13321 gctattcgtg tcttcgcaac ctacgccaag tgggatgaga aatggggtta tgactacaac
    13381 ggcgatagca aggttaaccc gaactacggc aaagccgttc ctgctgattt caacggcggc
    13441 agcttcggtc gtggcgacag cgacgagtgg accttcggtg cccagatgga aatctggtgg
    13501 taatagcaaa acctgggccg gataaggcgt ttacgccgca ttcggcaacc aacgcctgat
    13561 gcgacgcttg cgcgtcttat caggccgaca acggctgtca aatgtaggcc ggataaggcg
    13621 tttacgccgc atccggcata aaaacaggtt gtcattatct gaaaggggcg aaagcccctc
    13681 tgattatcgg gtttagcgcg ctattgcctg gctaccgctg agctccagat tttgaggtga
    13741 aaacaatgaa aatgaataaa agtctcatcg ccctctgttt atcagcaggg ttactggcaa
    13801 gcgcgcctgg aatcagcctt gccgatgtta actacgtacc gcaaaacacc agcgacgcgc
    13861 cagccattcc atctgctgcg ctgcaacaac tcacctggac accggtcgat caatctaaaa
    13921 cccagaccac ccaactggcg accggcggcc aacaactgaa cgttcccggc atcagtggtc
    13981 cggttgctgc gtacagcgtc ccggcaaaca ttggcgaact gaccctgacg ctgaccagcg
    14041 aagtgaacaa acaaaccagc gtttttgcgc cgaacgtgct gattcttgat cagaacatga
    14101 ccccatcagc cttcttcccc agcagttatt tcacctacca ggaaccaggc gtgatgagtg
    14161 cagatcggct ggaaggcgtt atgcgcctga caccggcgtt ggggcagcaa aaactttatg
    14221 ttctggtctt taccacggaa aaagatctcc agcagacgac ccaactgctc gacccggcta
    14281 aagcctatgc caagggcgtc ggtaactcga tcccggatat ccccgatccg gttgctcgtc
    14341 ataccaccga tggcttactg aaactgaaag tgaaaacgaa ctccagctcc agcgtgttgg
    14401 taggaccttt atttggttct tccgctccag ctccggttac ggtaggtaac acggcggcac
    14461 cagctgtggc tgcacccgct ccggcaccgg tgaagaaaag cgagccgatg ctcaacgaca
    14521 cggaaagtta ttttaatacc gcgatcaaaa acgctgtcgc gaaaggtgat gttgataagg
    14581 cgttaaaact gcttgatgaa gctgaacgcc tgggatcgac atctgcccgt tccaccttta
    14641 tcagcagtgt aaaaggcaag gggtaattac gccccacagt gctgattttg caacaactgg
    14701 tgcgtctcct ggcgcacctt tttttatgct tccttcctgg gatatgagcg attttttata
    14761 gtaactcact tcttcttcac taagaatatc cattatctca atgccttatc agagattctt
    14821 ttcctttcgc cggtagtgtc tggacattca ggctactttt ccaggttatt ttatttctgt
    14881 tatgcagagg ttttatgata agtcatatcc taaattctgg cggcaataac tctttgatga
    14941 aacatgatgt ggtgcaagga aataatatag tagatcttga tttactacgt aatttcaatg
    15001 gggtgccagg tttaaacaga gataacttta tttatatcag caatattttt tcaaatataa
    15061 aacaacggaa cgaaaaaatc atgcaataaa tttgtttcgt gaagtctcaa tcagtaatga
    15121 tactataagt gtaaaattct acagaaatga aaaaaattga atgcgcttgc aattttctga
    15181 tggataaaga tgcgcagggg tatatcgacc tgtctgattt ggatttaaca agttgtcatt
    15241 ttaaaggtga cgttatatcg aaggtgtctt ttttatcatc aaatctacaa catgtaacat
    15301 tcgaatgtaa agaaattggg gattgcaatt ttactactgc aatagttgat aatgtcatat
    15361 ttagatgtcg acgtttacac aatgtgattt ttatcaaagc gagtggtgaa tgtgtcgatt
    15421 tcagcaaaaa tattcttgat acagttgact tctcgcagag tcaacttact cagagtaatt
    15481 ttcgcgaatg tcagattaga aattcaaact tcgataattg ttatctttac gcttcgcact
    15541 tcaccagagc agagtttctg tctgccaaag aagtatcatt tattaaatcg aatatgacag
    15601 ctgttatgtt tgatcatgtg cgaatatcga cagggaattt taaagattgc attacagaac
    15661 aattggaatt aactattgat tattcagata t
//
LOCUS       DAZGLJ010000107        15505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000107 DAZGLJ010000000
VERSION     DAZGLJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.107"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..179)
                     /locus_tag="ACHK49_002884"
     CDS             complement(<1..179)
                     /locus_tag="ACHK49_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFO9263501.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVLERAVKRAD
                     SRFELIQKNVGYFFK"
     gene            complement(357..1268)
                     /gene="gsiD"
                     /locus_tag="ACHK49_002885"
     CDS             complement(357..1268)
                     /gene="gsiD"
                     /locus_tag="ACHK49_002885"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO9263502.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1271..2191)
                     /gene="gsiC"
                     /locus_tag="ACHK49_002886"
     CDS             complement(1271..2191)
                     /gene="gsiC"
                     /locus_tag="ACHK49_002886"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO9263503.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2209..3747)
                     /gene="gsiB"
                     /locus_tag="ACHK49_002887"
     CDS             complement(2209..3747)
                     /gene="gsiB"
                     /locus_tag="ACHK49_002887"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO9263504.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYIVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3767..5638)
                     /gene="gsiA"
                     /locus_tag="ACHK49_002888"
     CDS             complement(3767..5638)
                     /gene="gsiA"
                     /locus_tag="ACHK49_002888"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO9263505.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5625..6590)
                     /gene="iaaA"
                     /locus_tag="ACHK49_002889"
     CDS             complement(5625..6590)
                     /gene="iaaA"
                     /locus_tag="ACHK49_002889"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO9263506.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6794..8029
                     /gene="moeA"
                     /locus_tag="ACHK49_002890"
     CDS             6794..8029
                     /gene="moeA"
                     /locus_tag="ACHK49_002890"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO9263507.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRHGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            8029..8778
                     /gene="moeB"
                     /locus_tag="ACHK49_002891"
     CDS             8029..8778
                     /gene="moeB"
                     /locus_tag="ACHK49_002891"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO9263508.1"
                     /translation="MTELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8854..9516)
                     /gene="fsa"
                     /locus_tag="ACHK49_002892"
     CDS             complement(8854..9516)
                     /gene="fsa"
                     /locus_tag="ACHK49_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO9263509.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGNKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            9647..10546
                     /gene="ybiY"
                     /locus_tag="ACHK49_002893"
     CDS             9647..10546
                     /gene="ybiY"
                     /locus_tag="ACHK49_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO9263510.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10552..12984
                     /gene="ybiW"
                     /locus_tag="ACHK49_002894"
     CDS             10552..12984
                     /gene="ybiW"
                     /locus_tag="ACHK49_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO9263511.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13130..13945
                     /gene="ybiV"
                     /locus_tag="ACHK49_002895"
     CDS             13130..13945
                     /gene="ybiV"
                     /locus_tag="ACHK49_002895"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO9263512.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14097..15362
                     /gene="ybiU"
                     /locus_tag="ACHK49_002896"
     CDS             14097..15362
                     /gene="ybiU"
                     /locus_tag="ACHK49_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO9263513.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVISPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         3767 a         4274 c         3938 g         3526 t
ORIGIN      
        1 tctttaaaga aatagccaac gtttttctga ataagttcaa agcggctatc ggcacgtttt
       61 accgcgcgtt ccaataccag ttgatagtta atgatgaaaa aaagaccaat aaatgacagt
      121 gctgccaata aaaataaaat tatcagcctt atctttatct tttcgtataa actcagcatg
      181 ttaatacatt taacaaaata actatctgaa cgctgtgccg ctgcaaaaat agtcccttgc
      241 tgcggctttt taaccaaatt acccgattct acaaaatcag cagaaagtta caacacttgc
      301 ttagggcaat aatttcctgt gaggtgatta ccctttcaag caatattcaa acgtaactat
      361 cctttaattt tcggatccag cgcatcgcgt aaaccatcgc ccaacaaatt gaacgccagt
      421 acggtcagaa aaatagccag ggccggaaaa acagcgacat gcggcgcgat aaccatatcc
      481 gctcgtgcct cattgagcat tgctccccac tctggtgtcg gcggctgcgc accgaggccg
      541 agaaatgaca ggctggcggc agagataatc gaggtgccaa tgcgcatggt gaaaaacacc
      601 acgatagacg agacggtccc aggcaagata tgacgcaata aaatggtcat atcgctggca
      661 ccaatactgc gtgctgactc aataaaggtt tgctgtttca acaccagcgt gttgccgcgc
      721 accagtcggg caaacgcggg gatggaaaaa atggcgactg cgataatcac gttagcaatg
      781 ccgcttccca acaccgcaac aacagcgatc gccagtaaaa tacccgggaa ggcaaacagc
      841 acatcgcaaa tgcgcatgat caaccgatcc caccagcctt cataatatcc agccaacaag
      901 cccagcaacg tcccgatcgc cgcaccgata aacacggcaa acacgcccgc cgccagcgag
      961 atttgcgcac caaccaggac acggctgaaa atgtcgcgcc ccagtgaatc gacgccaaac
     1021 cagtgctgca aagaaggtcc gttattcaga ttgtcataat caaaataatt ttcggcgtca
     1081 tagggagcga tccagcgtgc aaaaatggcc accacaatca ataaaataac gaataatgcg
     1141 gcggtcatcg ccatatgctg acggcgaaat cgtcgccaga attcatgcca cggtgtacgc
     1201 acctggtcag gtttgaccag tggcatggcg tttaacaccg cctgacgtcg ccagttaaat
     1261 agtcgcatcc ttacttgtac ctgatagccg ggttaatggc ggcgtaaagc acatccacca
     1321 ctaagttgat aagaataaat tccagcgaga aaagcagaat ttccgcctga atcaccgggt
     1381 aatcgcgcat ttctacggag tcaacgagta agcgtccaag tccgggccag ttgaaaactt
     1441 tctccaccac gatagaacca ccgagcaaaa agccaaactg taagcccatc atggtcacta
     1501 ccgggatcat cgcgttacgt agcccgtgtt tgaggacaac ccaggtttcg ctcacccctt
     1561 tcgccctcgc ggtacgcata taatcttcgc ttaaaacatc gacaaacgac gcgcgggtaa
     1621 agcgcgccat cacggctgcc actgccgcgc cgagcgtcag ggagggtaaa atgtagtgct
     1681 gccaactgtc tgctcccacg gtaggcagcc agcccagttc aacggagaat acctgaatta
     1741 aaagcatccc cagcgcaaat gccggaaacg agatccccga caccgcaatg gtcatactca
     1801 atcgatccgg ccaacgatta cgccagacgg cggcgataat tcccgccgcc ataccaaata
     1861 tcaccgccca gaccatactg gttatggtca gccacagcgt tggcataaag cggctggcaa
     1921 tctcatcggc aaccggacga cgcgacacca tcgacaggcc aaaatccccc tgcacagcat
     1981 tgctgatata gtgccagaac tggtgataca gcggctgatc caaccccagc tgctgacgca
     2041 ccagttctat aacctgcgca tcagcttcgg gcccggcaat caatcgcgcc ggatcgccgg
     2101 gcagcatatg cacaaataaa aacaccagca ccgagacgat aaacagcgtc ggaatcaacc
     2161 ccagtaagcg tttgataacg taattaagca ttccactccc tgcgttgctt attgcaaatc
     2221 cgcgtcttca aagctgaagc cggtgtctgg catgatccaa aaaccggtca ggtttttact
     2281 gtgtgccgac accagttttt ctaccaccag cgggatccac ggcgactctt gccagatgat
     2341 atcctgcgcc gccttataca agcgggtctt ttccgccgga tcattagttt tcagtgcctg
     2401 agccaggaag tcgtccacct gtttattgct gtaaaatgcg gtattaaaca gcgtcggtgg
     2461 ccagttttgc gaggcgaaca gtggcgatag cgcccagtcc gcttcgccgg ttgaagccga
     2521 ccagccagtg tagaacatcc gcacgccgct ctctttttgc cctttacctt caacttctgc
     2581 cgcccgctgt ccggcatcca tcgcggtcac ctgggcttta atcccgacct gcgctaactg
     2641 ctgctgggta aattgcagca ctttctgcgc ggtgctgtgg ttatgtgacg accacagcgt
     2701 ggtactgaaa ccgttgggat atcccgcctc tttcagtaat tcgcgcgctt tcactggatc
     2761 gtaaggccac ggtttataac tttgcgcgta ggcgatactt ggcggtacca caccagtagc
     2821 tggcgttgca tagcccgcaa aggcaacttt caccagcgcc ggacggttaa tggcgtaatt
     2881 cagcgcctca cggaccttcg ggttatcgaa cggcttttgc gtcacgttca tactgatata
     2941 acgctgcata attgacggac tggccatcaa ctcgatattt ttgtttttct ccagcagtgt
     3001 ggcctgctcg tagggaatgg ggaaagcaaa ctgcgcttca ccggtttgca gcattgccgc
     3061 gcgggtgttg ttatccgcca ccggacgcca ggttatgctg tccagtttgg gcaatcctgg
     3121 ctgccagtaa cccgcgaatt ttttcacctt cacaaaatcg gtctgattcc aggtatccag
     3181 ttcatacggt ccggttccca ccggatgaaa accaatctcc ttgccatatt tttccagcgc
     3241 tgccggtgaa atcatcgcgg tcgccggatg ggcaagaata ttaataaacg ctgagaacgg
     3301 ctgtttgagg gtaatcttta ccgttgtcgg atcgatcgcc tccgttttgg caatattctt
     3361 atacaggtta tagcgtttaa gatgattcgc cggatcgctg gcccggtcca gattcgcttt
     3421 taccgccact gcgttgaaat cggtgccatc ctggaattta attccttccc gcaatttcac
     3481 gatgtaagta atgccgtcat cggaaacggt ataactctcc gccagcacgt ttttcagttt
     3541 catctcttta tccagaccga acagcccctg gtaaaacgat ttcgctacgg cctgagataa
     3601 cgtgtcattt gcgtcatacg gatcgagcgt ggtgaaattc gatcctaccg ccaccaccac
     3661 atctttggca gcgaatgcac aagatgccat caacgctgtc gcaatgccca gcgccactaa
     3721 cccactacgg tgtacagctc ttgccatttt attctccgcc tgaatgttat ctacgcatga
     3781 atgcgtattc tgattgtggt tgtgcgacgt aatgccccgg cccgacgcat tgcaacgaga
     3841 cggctgccac ctcttcgcca cgcagatgaa tattgctggg aagatcgtcc gacagcagta
     3901 cacgctgcgg tcgttgtcgg gacggttcag cgaccggaac tgccgccagt aatttacgcg
     3961 tataaggatg ctgcgggttt tcgaagaccg cgcgccgtgg accaatttca acaatttgcc
     4021 cgagatacat caccgccaca cgatgactaa tccgctctac cacggccata tcgtgggaga
     4081 taaacagata cgcaatgccg aaatcacgct ggagatcgag caacaagttg ataatctgcc
     4141 cgcgaataga aacatccagc gccgaaacgg cttcgtcggc aatgatcact tttggattca
     4201 atgccaacgc gcgagcaatg cagatgcgct ggcgctgacc gccggaaaac tcatgcgggt
     4261 aacgccaggc atgttcaggt aacaggccca cgcgctccag caaccacgca acgcgtgcag
     4321 ccgcgtcttt acctggcaat aaaccgtgta cacgcagcgg ttcgataatc gaatcaccga
     4381 tggtctgacg tgggtccagc gaagcgtaag ggtcctgaaa aataaactga atatcccggc
     4441 gcaatgcctg aagtttgccg ggtgacaagg tatcgattct ctgaccgtta aagataattt
     4501 cgccgccctg cgattcgacc aggcgcagca acgcccgccc ggtagtggat ttaccgctgc
     4561 cagactcgcc caccagcgat agcgtttcgc caggccagag atcaaaacta actttctcaa
     4621 cggcatgcac ttcccgcgtt acgcgattca acaaaccgct gcgcaaaggg aaacgggtga
     4681 caagattacg cactcgtaaa acaggttcgc catccaccac cgttttctgc tcgatggggg
     4741 gggcctgttt cgctggatgt tcaagcgata tcaacgggaa acgtcgggga taatctaacc
     4801 ctttcatcgc accaagttgc ggaacagcag ctaacagcgc acgggtgtaa ggatgttgcg
     4861 gtgcatgaaa aatctgttcg acggtacccg tttccaccgc ctcgccctga tacatcacca
     4921 gtacccgatc ggcaatctct gccaccacgc ccatatcgtg agtgataaag ataacgccca
     4981 tcgacatctc tttttgcaat actttgatta attgcaggat ctgcgcctga atagtgacat
     5041 ccagcgcggt ggttggctca tcggcaatca gcaccgccgg gcggcatgac agcgccatcg
     5101 caatcatcac tcgctggcgc atcccgccag agagttgatg cggataacgt gaaagaatgg
     5161 tttgtgcctc aggaatgcgt acctgatcca gcatccgctt cgcctcgacc atcgcttctt
     5221 cacgactggc gttctgatgc agacgaattg attcggcaat ctgttcaccc acagtaaata
     5281 ccgggttcag cgatgtcatc ggctcctgaa atatcatcgc catatccgca ccgcgaacat
     5341 ggcgcatttg tgcagcgctc tgctcgctaa gttcaatcac ttcgcgactg cgccgctgca
     5401 acagcatttt atcgcactgt actaaaccgc ccgcctgttc caacaggcgc atcaatgcca
     5461 acgcagtcac tgacttaccg gagccggatt cgccaacaat tgccagcgtc tcaccgcgtt
     5521 gcagactaaa agagagattg cggaccgcag ctattttctg ctggtcctgc ataaaggcaa
     5581 tattcagatt ttcaaccgcc agcacattac cggcatcaag ttcatcactg tgtggcaacg
     5641 gtgtcccctt tttcacggta gataccggtg gttggcgtat cgcctgcgta gccccaggcg
     5701 cgatacattc cttcggtgtt aaacggtagc gcgacattcc cttcatggtc gatagcgatt
     5761 aagccaccgc taccgccaag cgcagggagt ttttccatta ctacccgctc gcaggcttcc
     5821 gcgagactta atccgccgta atccattaac gcggcgatgt catatgccgc cagcgcgcgg
     5881 atgaagactt cgcccgtgcc ggtacaagaa accgccacac tggcgttatt ggcgtagcat
     5941 ccggcaccca ctaagggact atcgccaact cgtccgggta atttattggt cattccgcct
     6001 gtggacgtgg ctgccgccaa attgccgtct aaatccaacg ccacggcccc cacggtgccc
     6061 attttctgtt tttcatccag tggcgcaccg ctatggtcga ggacggttgc cccttccttg
     6121 cgcgctgcca gtagttgttc ataacgcaaa ggcgtggaga aaatctccgg cgagacgcgc
     6181 tccatgccac gagcaaacgc aaaattttct gccccttcgc caatcatcat cacatgcggg
     6241 ctttgctcca tcaccagccg ggcggcaaga accggattac gcagatgact aacgcccgcc
     6301 accgcaccgg ctttcagggt gttaccatcc atcacacagg cgtccagttc atgggtttca
     6361 tcacgcgtaa agacagcgcc aattccggcg ttaaacagtg gacactcttc cagcagacgc
     6421 accgcttccg tcaccacatc cagcgcactt tcgcccgctt ccaacatttt ctgcccggtt
     6481 tcaacaatgg cagacaacgc ctcaatgtag cgtaattcct gttgcagact catctgcgcg
     6541 cggctaattg cacctgcgcc accatgaatt gcaatgactg ctttgcccat agtattcgtc
     6601 cattaagggt ggctcgccac ttttgctata aatatcagaa tttttatgaa ttactcattc
     6661 aatttttgaa tatagaaaga tggatatggc atgtaaaggc aggagccaac gctacgcccg
     6721 aggtggcata cagtctgtgg cgtttttctg acataatagg caaattcgat tttgcctccg
     6781 caggagtgtt ttcatggaat ttaccaccgg attgatgtcg ctcgacaccg cgcttaatga
     6841 gatgctttct cgcgtcaccc cactgaccgc ccaggaaacg ctgccactgg tacagtgttt
     6901 tggtcgtatt ctggcgagcg atgtcgtttc gccgcttgat gttccggggt ttgataactc
     6961 cgcaatggac ggctacgcgg tgcgtttagc cgatattgcc tccaggcaac cgctgcccgt
     7021 tgccggtaaa tcctttgccg gtcagccgta tcatggtgaa tggcctgcgg gcacctgcat
     7081 tcgtattatg accggtgcgc cggtgccgga aggctgcgaa gcggtggtga tgcaggagca
     7141 gactgaacaa acggacaatg gcgtgcgttt tactgctgaa gtgcgtagcg ggcaaaatat
     7201 tcgccgtcac ggtgaagata tctctgcagg tgcggttgtt ttcccggcgg gaactcgcct
     7261 gactaccgca gagctgccag taattgcttc actggggatt gccgaagttc cggtgattcg
     7321 taaagtgcgt gtagcgcttt tttctaccgg tgatgaactc cagttgcccg gtcagccgct
     7381 gggcgacggc caaatctacg acaccaaccg tctcgccgta cacctgatgt tagaacagct
     7441 gggttgcgag gtaatcaact tagggattat ccgcgacgat ccccatgccc tgcgcgccgc
     7501 atttattgaa gccgacagcc aggcggatgt agtgatcagt tccggcggtg tttcagtggg
     7561 tgaagcggat tacaccaaaa cgattcttga agagctgggg gagatcgcct tctggaagct
     7621 ggcgattaaa ccaggtaaac cgttcgcgtt cggtaaactc agcaatagct ggttctgcgg
     7681 cctgccgggc aacccggttt cagcgacgct gaccttctat caactggtac agcctttgct
     7741 ggcaaaacta agcggcaaca ccgccagcgg cctgcccgcg cgccagcgcg tacgcacagc
     7801 gtcccgcctg aaaaaaacgc caggacgtct tgatttccag cgcggcgtgc tgcaacgcaa
     7861 cgccgacggt gaacttgaag tgacgaccac cggacatcag ggttcacata tatttagctc
     7921 ctttagtctc ggcaactgct ttatcgtgct ggaacgcgat cgcggcaatg tggaagtggg
     7981 cgaatgggtg gaagtagaac cgtttaacgc gttgttcgga ggcctgtaat gacggaactc
     8041 agcgatcagg agatgctgcg ctacaaccgg caaatcattc tgcgcggttt tgattttgac
     8101 ggtcaggagg cgctaaaaga ttctcgtgtg ctggtagtcg gcctcggtgg actcggctgc
     8161 gcagcttcgc agtatctggc aagcgccggt gtcggcaacc tgacgctgct cgacttcgac
     8221 tcggtttcgc tctcgaatct gcaacgccag acgctgcaca gtgatgctac ggtcgggcaa
     8281 ccgaaggtag aatccgcccg tgacgccatg acgcggatca acccacatat cgcgattacg
     8341 ccagtcaatg cactgcttga tgacgctgaa cttgctgcga tgattgcaaa acacgatctg
     8401 gtactcgact gtacggataa cgttgcggtg cgtaatcaac tgaacgctgg ctgttttgcc
     8461 gcgaaggtgc cgctggtttc cggcgcggca attcgtatgg aagggcaaat cacggtcttc
     8521 acttatcagg acggcgaacc gtgctatcgc tgccttagcc gtttgtttgg cgagaacgca
     8581 ttaacctgcg tagaagcagg cgtgatggca ccgttgatcg gcgtaattgg ttcgttgcag
     8641 gcaatggaag cgatcaaact gctggcaggt tatggcaaac cggccagcgg gaaaatcgtc
     8701 atgtacgatg caatgacctg tcagtttcgc gaaatgaaac tgatgcgcaa tccggggtgt
     8761 gaggtgtgcg ggcagtaatt gccacctcca ggccagataa gacgcggcac tccgccacgt
     8821 catcagcaat ttatgaggat gcagaacgtc cggttaaatc gacgttctgc caaacgctcc
     8881 cagccagtcc tgttcaaact tcgccacagc ggcttccacc gccggatagc taatcatctg
     8941 ttgtgccaca tccagtggca gcgtaattga ttcacatcct gccagtaagc agtccagcgc
     9001 ctgacgcggg gttttgaaac tcgctgccag cactttcgcc tgcggcgcat gcattttcaa
     9061 taactggtgt aagtcggtca cagtctgaat gccgctacca ccctgagcat caatacgatt
     9121 aacgtaaggc gcaacatatt ccgcacctgc cagcgccgac agcagccctt gtgctgcgcc
     9181 atataccgcg gttcccagcg ttggaatccc ttccgctttt aacatcttaa tagctgccag
     9241 cccctcggcg gtcaccggaa ctttcaccac gatatccgca ataatagaac gcaatttacg
     9301 tgcgtcattc accatccctt cagcagtggt agccattacc tgggcaaaca gacgcccctg
     9361 accgcccatc gcttcatgaa gttgcggaag cacaacatcc agcggtttgt tacccgcggc
     9421 gataatgctt gggttagtgg tcacacccgc cagcggaaaa atacgtgaca gcgccttcac
     9481 cgcaacaacg tctgaagtat ccagatacag ttccataacc atcctcaaaa tgtacttttt
     9541 gtctgaatgt agactatcac gggaaaactc tctgaacagt tgacgcacat caatctaact
     9601 ttcattcgaa agcattttaa tgtttgaacg aaacagagag gcaactatga ttttcaatat
     9661 tcagcgctac tcgacccatg atggccccgg tatccgcacg gtcgtatttc ttaaaggctg
     9721 ttcgctgggc tgccgctggt gtcagaaccc ggaaagccgc gcccgcacgc aggatctgct
     9781 gtatgacgca cgactgtgtc tggaaggctg cgagctgtgc gctaaggccg cgccggaagt
     9841 gattgagcgc gcgctgaatg gtttgcttat tcatcgggaa aagttaaccc cggagcatct
     9901 gacggcgtta accgactgct gtccgacaca ggcattaacc gtgtgtggtg aagtgaaaag
     9961 cgttgaggag atcatgacga ccgttctgcg cgataaaccg ttttatgatc gcagcggcgg
    10021 cggtttaacg ctttcgggtg gtgagccctt tatgcagccg gaaatggcga tggcgctact
    10081 gcaagccagc cacgaggcag gcattcatac tgcggtagaa acctgtctgc atgtgccgtg
    10141 gaaatatatc gccccttctc tgccctatat cgatctgttt cttgccgatt taaaacacgt
    10201 tgccgacgcg ccgtttaaac agtggaccga cggtaacgcc gccagagtgc tggataacct
    10261 gaaaaaactc gccgcagcgg gcaaaaaaat cattatccgc gtgccgctga ttcagggctt
    10321 taatgccgac gaaacctctg taaaagccat taccgatttt gccgccgacg agctgcacgt
    10381 tggcgaaatt cattttctgc cctaccacac gctgggcatc aacaaatatc acttacttaa
    10441 tctgccctat gacgccccgg aaaaaccgct tgatgcgcca gaactgctcg actttgccca
    10501 gcagtatgcc tgccagaaag ggttaaccgc gaccttacga ggataacaac catgaccaca
    10561 ctgaaactgg acacgctcag cgaccgcatt aaagcgcaca aaaatgcgct ggtgcatatt
    10621 gtgaaaccgc cagtctgtac cgagcgcgcg cagcactata ccgagatgta tcaacaacat
    10681 ctcgataagc cgatcccggt acgtcgcgcg ctggcactgg cgcatcacct ggcgaatcgc
    10741 accatctgga tcaaacacga tgagttgatc attggcaacc aggcaagcga agttcgcgcc
    10801 gcgccgatct tcccggaata tactgtctcg tggatcgaaa aagagattga tgatctggca
    10861 gatcgtcccg gtgctggctt tgcggtgagc gaagagaaca aacgcgttct gcatgaagtg
    10921 tgcccgtggt ggcgcggtca gaccgtacag gatcgctgct acggcatgtt taccgatgag
    10981 caaaaaggtc tgctggcgac cggaatcatt aaagcggaag gcaatatgac ctccggcgat
    11041 gcgcacctgg cggtgaattt cccgctgctg ctggaaaaag gtcttgatgg tctgcgtgag
    11101 aaagtagcgg aacgtcgctc gcgcatcaat ctgacggtgc tggaagattt acatggcgaa
    11161 caattcctga aagcgattga tatcgtgctg gtggcagtca gtgaacacat tgaacgtttc
    11221 gctgccctgg cgcgtgaaat ggctgctacc gaaactcgcg aaagccgtcg cgatgaactg
    11281 ctgacgatgg cagaaaactg cgatcttatc gcccaccagc cgccgcagac tttctggcag
    11341 gcgctgcaac tgtgttactt catccaattg attttgcaga tcgaatctaa cggtcactcg
    11401 gtatcgtttg gtcgtatgga ccagtatctc tacccgtact atcgccgcga cgttgaactc
    11461 aaccagacgc tggatcgcga acacgccatc gagatgctgc atagctgctg gctgaaactg
    11521 ctggaagtga acaagatccg ctccggctca cactcaaaag cctctgcggg aagtccgctg
    11581 tatcagaacg ttaccattgg cggacaaaat ctggttgatg gtcaaccaat ggatgcagtg
    11641 aatcccctct cttacgcgat cctcgaatcc tgcggtcgcc tgcgttccac tcagcctaac
    11701 ctcagcgtgc gttaccatgc aggaatgagt aatgatttcc tcgacgcctg cgtacaggtg
    11761 atccgttgcg gcttcgggat gccggcgttc aacaacgacg aaatcgtgat cccggaattt
    11821 attaaactcg gtattgaacc gcaggacgct tacgactacg cagcgattgg ttgtatcgaa
    11881 accgccgtcg gtggcaaatg gggctatcgc tgtaccggca tgagctttat caacttcgcc
    11941 cgcgtgatgc tggcggcgct ggaaggcggg cgtgatgcca ccagcggcaa agtgttcctg
    12001 ccacaagaaa aagcgttgtc ggcaggcaac tttaacaatt tcgatgaagt aatggacgcg
    12061 tgggatacgc aaatccgtta ttacacgcgt aaatcaatcg aaatcgaata tgtcgtcgac
    12121 accatgctgg aagagaacgt gcacgatatt ctctgctcgg cgctggtgga tgactgtatt
    12181 gagcgagcga aaagtatcaa gcaaggcggc gcgaaatatg actgggtttc tggcctgcag
    12241 gttggcattg ccaacctcgg caacagcctg gcggcagtga agaaactggt atttgaacag
    12301 ggcgcgattg gtcagcaaca gctggctgcc gcactggcgg atgacttcga tggcctgact
    12361 catgagcaac tgcgtcagcg gctgattaac ggtgcgccga agtacggcaa cgacgatgat
    12421 actgtcgata cgctgctggc tcgcgcttat cagacctata tcgacgaact gaaacagtac
    12481 cataatccgc gctacggtcg tggtccggtt ggcggcaact attacgcggg tacgtcatct
    12541 atctccgcta acgtaccgtt tggcgcgcaa actatggcaa cgccggacgg gcgtaaagcc
    12601 cacaccccgc tggcagaagg cgctagtccg gcctctggta ctgaccatct cggccctact
    12661 gcggtcattg gctcggtggg taaactgcct acggcagcga ttctcggcgg cgtgttgctc
    12721 aaccagaaac tgaatccggc aacgctggag aacgaatctg acaagcagaa gctgatgatc
    12781 ctgctgcgca ccttcttcga ggtgcataaa ggctggcata ttcagtacaa catcgtttcc
    12841 cgcgaaacgc tgctggaggc gaaaaaacat ccggatcagt accgcgatct ggtagtgcgt
    12901 gtcgcgggtt attccgcctt cttcaccgcg ctctctccag acgctcagga cgatatcatc
    12961 gctcgtactg aacatatgct gtaatctctt cggcccggct gcggcgtcgc cgggctaaat
    13021 cattcacatc atcaatttca tccttacttt cattcgaaat ataatttgtg ctctgcgtca
    13081 cattgttatt acactgttac ggtttcattt tcgagccagg agcacaccta tgagcgtaaa
    13141 agttatcgtc acagacatgg acggtacttt tcttaacgac gccaaaacgt acaaccaacc
    13201 acgttttatg gcgcaatatc aggaactgaa aaagcgcggc attgagttcg ttgtcgccag
    13261 cggtaatcag tattaccagc ttatttcgtt ctttcctgag ctaaaggatg agatctcttt
    13321 tgtcgcggaa aacggcgcac tggtttacga acatggcaag caactgttcc acggcgaact
    13381 gacccgacat gaatcgcgga ttgttattgg cgagttgcta aaagataagc aactcaattt
    13441 tgtcgcctgc ggtctgcaaa gtgcatatgt cagcgaaaat gcccccgaag catttgtcgc
    13501 actgatggca aaacactacc atcgcctgaa agctgtaaaa gattatcagg agattgacga
    13561 cgtactgttc aagttttcgc tcaacctgcc agatgaacaa atcccgttag tgatcgacaa
    13621 actgcacata gcgctcgatg gcattatgaa acccgtcacc agtggttttg gctttatcga
    13681 cctgattatt cccggtctac ataaagcaaa cggtatttcg cggttactga aacgctggga
    13741 tctgtcaccg caaaatgtgg tagcgattgg cgacagcggt aacgatgcgg agatgctgaa
    13801 aatggcgcgt tattcctttg cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg
    13861 ttacgctacc gatgataata atcatgaagg cgcgctgaat gtgattcagg cggtgctgga
    13921 taacacatcc ccttttaaca gctgacctct ctaccggagc cgtgcgctcc ggttttccct
    13981 tctttttaca tcctgtacat cacatcaaat cattaaactt gcattaactt atttataact
    14041 tatattgtca catgcaaatc agaatggttt cgaatgaaag ttaatgagag gtggttatgg
    14101 cttctacttt taccagcgac acattgcctg ccgatcacaa agcagctatc cgtcagatga
    14161 agcacgcgct gcgggcgcag cttggcgacg tccagcagat ctttaatcag ctaagcgatg
    14221 acattgccac gcgagtggct gaaatcaacg cactcaaagc acagggcgat gccgtctggc
    14281 cggtgctgtc ttatgccgat atcaaagcag gtcatgttac tgcagagcag cgcgaacaga
    14341 ttaaacgtcg cggttgtgcg gtgataaaag gccatttccc ccgcgaacaa gcgctaggct
    14401 gggatcagtc gatgctggac tatctggacc gcaaccgctt tgacgaggtc tacaaaggcc
    14461 ccggcgataa tttcttcggg acgctcagcg cttcacgtcc cgagatttac cccatctact
    14521 ggtcgcaggc gcaaatgcag gcccgccaga gtgaagaaat ggcgaatgcg cagtcgtttc
    14581 tcaatcgtct gtggacattt gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg
    14641 tcatctcccc tgaccgtatc cgccgccgtc cgcccggaac gacctccaaa ggtcttggag
    14701 cgcataccga ctccggggcg ctggaacgct ggctgcttcc agcgtatcag cgcgttttcg
    14761 ccaacgtctt taatggcaat ctggcgcaat atgatccctg gcatgcggca catcgtacgg
    14821 aagttgaaga gtacacggtg gacaacacca ccaaatgttc cgtgtttcgg acattccagg
    14881 gctggacagc gctctctgat atgctgcctg gtcaggggct gctgcacgtt gtgcccattc
    14941 ctgaagccat ggcgtacgta ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac
    15001 tgtgcggcgt agcgcccgga agagtgttgc cggtatcaga gcaatggcat ccactgttaa
    15061 ttgaggcgtt aaccagcatt ccaaaactcg aggccggaga ctccgtctgg tggcactgcg
    15121 acgtcatcca ttccgttgcc cccgttgaaa atcaacaggg ttggggcaac gtgatgtaca
    15181 ttcctgcggc accgatgtgc gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac
    15241 tggaaaaagg cgcatcgccg ggcgacttcc cgcgcgagga ctatgaaaca aactgggaag
    15301 gacgctttac gcttgccgac ctcaacattc acggtaagcg agcgttgggc atggatgttt
    15361 gagagttaat ggaagtgaat tgatgtggga ttaagaaaat cgattcgacg ttactcagat
    15421 tgctgacaag tgcgctttct taatgcctga tgcgctacgc ttatctggcc tacaaagtcc
    15481 tgcagattca atatattaca ggtac
//
LOCUS       DAZGLJ010000108        15245 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000108 DAZGLJ010000000
VERSION     DAZGLJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.108"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            445..1101
                     /gene="pphA"
                     /locus_tag="ACHK49_002897"
     CDS             445..1101
                     /gene="pphA"
                     /locus_tag="ACHK49_002897"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO9263514.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(1102..1293)
                     /gene="yebW"
                     /locus_tag="ACHK49_002898"
     CDS             complement(1102..1293)
                     /gene="yebW"
                     /locus_tag="ACHK49_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO9263515.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(1398..1634)
                     /gene="yebV"
                     /locus_tag="ACHK49_002899"
     CDS             complement(1398..1634)
                     /gene="yebV"
                     /locus_tag="ACHK49_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO9263516.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1752..3191)
                     /gene="rsmF"
                     /locus_tag="ACHK49_002900"
     CDS             complement(1752..3191)
                     /gene="rsmF"
                     /locus_tag="ACHK49_002900"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO9263517.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQATAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(3271..5904)
                     /gene="yebT"
                     /locus_tag="ACHK49_002901"
     CDS             complement(3271..5904)
                     /gene="yebT"
                     /locus_tag="ACHK49_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO9263518.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSVSGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5873..7156)
                     /gene="yebS"
                     /locus_tag="ACHK49_002902"
     CDS             complement(5873..7156)
                     /gene="yebS"
                     /locus_tag="ACHK49_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO9263519.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7286..7783
                     /gene="msrC"
                     /locus_tag="ACHK49_002903"
     CDS             7286..7783
                     /gene="msrC"
                     /locus_tag="ACHK49_002903"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO9263520.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7880..8578
                     /gene="proQ"
                     /locus_tag="ACHK49_002904"
     CDS             7880..8578
                     /gene="proQ"
                     /locus_tag="ACHK49_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO9263521.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8598..10646
                     /gene="prc"
                     /locus_tag="ACHK49_002905"
     CDS             8598..10646
                     /gene="prc"
                     /locus_tag="ACHK49_002905"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO9263522.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPDPVK
                     "
     gene            10838..11719
                     /gene="htpX"
                     /locus_tag="ACHK49_002906"
     CDS             10838..11719
                     /gene="htpX"
                     /locus_tag="ACHK49_002906"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO9263523.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(11765..13138)
                     /gene="yebQ"
                     /locus_tag="ACHK49_002907"
     CDS             complement(11765..13138)
                     /gene="yebQ"
                     /locus_tag="ACHK49_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9263524.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPARSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLKPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            13315..14106
                     /gene="kdgR"
                     /locus_tag="ACHK49_002908"
     CDS             13315..14106
                     /gene="kdgR"
                     /locus_tag="ACHK49_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO9263525.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(14249..14488)
                     /gene="yobH"
                     /locus_tag="ACHK49_002909"
     CDS             complement(14249..14488)
                     /gene="yobH"
                     /locus_tag="ACHK49_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO9263526.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            14647..14790
                     /gene="mgrB"
                     /locus_tag="ACHK49_002910"
     CDS             14647..14790
                     /gene="mgrB"
                     /locus_tag="ACHK49_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO9263527.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            14865..15152
                     /gene="yebO"
                     /locus_tag="ACHK49_002911"
     CDS             14865..15152
                     /gene="yebO"
                     /locus_tag="ACHK49_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO9263528.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
BASE COUNT         4017 a         3686 c         3900 g         3642 t
ORIGIN      
        1 aagaacgtgc cacggctggc tgatggacgt tcgatagcgc gagtttgaat gaaaatcagc
       61 cggagatgat tttacataat tgctacggaa ttattcaata caggaattgc ttgtgtatgc
      121 atggattgac ctgaaatatt cccgaaaatt tctctaaaaa actcgaaaaa aatggtaact
      181 aattgaatgt attaatatgt aatagtacgt gttagagatt aaaagatgag cataaattta
      241 tttaacacat taattctaaa agattttgtt gtttgttgac gaaaacagga atcgtgttcg
      301 gtctcttttt atctgttaaa agccagaagc atttccttcg ctgactttat agtcaaccat
      361 aacacacact ctactgtctg agtccagcgt tttttaacat tcttgttaag attatgtgat
      421 ctttagcgcg ggaggaaaat attgatgaaa cagcctgcgc ccgtttatca gagaattgcg
      481 ggtcatcaat ggcgacatat ctggctttct ggcgatatac acggttgtct tgagcagttg
      541 cgccgcaaat tatggcattg tcgttttgat ccgtggcgag atttacttat ctcagtagga
      601 gacgttatcg atcgtgggcc gcaaagttta cgttgtctgc agttactgga acaacattgg
      661 gtttgtgcgg taagaggcaa tcatgaacag atggcgatgg atgcgctggc atcccagcag
      721 atgtctttgt ggttgatgaa tggcggcgac tggtttattg cgctggcaga taatcaacag
      781 aaacaagcga aaacggcgct ggaaaaatgt cagcatttgc cctttattct tgaagtacac
      841 agtcgtaccg gcaaacatgt tattgctcat gccgattatc cagatgatgt ttatgaatgg
      901 caaaaggacg ttgatttgca tcaggtcttg tggagccgct cgcgattagg tgaacgccaa
      961 aaagggcagg gaattacagg tgctgatcat ttctggtttg gtcatacacc gttgcgacat
     1021 cgcgtggata ttggcaacct gcattatatt gataccggtg ctgtctttgg gggcgaactg
     1081 actcttgtgc aattgcaata attaaaaatc accatactcc tgtgctggtc gccagaaacc
     1141 atctataaaa tcctcaatcg gaaaacaacc gccatggcgg atccgttgat cgctcataga
     1201 ataaagacac tgctgttccg tgttgtagac atccacaaca atatcttcac aaccgccatc
     1261 caggtagcaa acaaaaagta ccagcgcgaa catttcatcc ccgaagtgtg gtgccgtacc
     1321 gttaagttta ggagagattt tacaacgggg gaataaccag gacaaataac ccgccatgcc
     1381 gacgggttct ttttggatca ggcaagacgc ataatccagg catcagattg ttgatcgtaa
     1441 cgggtacggt aaaggacaga gtgttcgcca tttagcaggg cagggatact ggtttcagca
     1501 tcccaggcat cgagttgcat acataaatcg gggtcagatt tacaaggaat ggttaacgtt
     1561 cgatcacccg gcgattcacc gtgtaattcg ccgtcgtcga tttcaaaagc gcctatgcgc
     1621 acactggttt tcatcttcgt gctctccatt tagctgccga ttgtggtacg accagtccga
     1681 tcgcaccatt tttcagccta gttgaggcag ccgaatgtgc cagtcaaaaa gtgcgctttt
     1741 tttgtgagct gtcaggcgtt accggtaaaa agtttcccat cgcgcaccag ttcacgcgga
     1801 tagctgtttt tcaatcgcga accaatccgt ttggctaaac caatcggttg atgctggaaa
     1861 gtaaccaata catcatccgc cactggcgcg gcttgcgggt aaacatcgcg cccgcgatac
     1921 cactcctccg cttcctgcgg tgtcagctcg aatgcattca tattatcggg tgaggcaaga
     1981 gcaataaccg cttcatgctg ccagcgataa cctttgttgt gcgtctcggc aagtttaatc
     2041 cccaaccgag aaaatcggac tttaccgatc agggcttcaa tgcccaccgg gaacaaccac
     2101 agttctttgt cacgctgcca gaggcgcagg ttttcatccc agtttaagcc aacacctgca
     2161 gtcgcctgac gaatttgtcc agcttcgcga tctttcaccg ggctgaacgg aaaattaccg
     2221 actttgtatt tgggggcggg taaggcggga atcgcctgag ttttacgcag acgagcaacg
     2281 aagaagcctt cgcagtcgta aatttgtggg aaaacatgca aaaagccttc ttcggtcagc
     2341 gctttgtttg caccagggaa gagatcgcca agtggtaaaa actctactgc gtcggggtaa
     2401 gtctctttca gccacaggca aacggcttcg ttttcttcct ggtttaaggt acaggtcgag
     2461 taaaccagcg taccaccagg acgtaatgca tgaaaggcgc tgtcgataag ctcccgttgt
     2521 gtagctgcga tttcctgatt gctttctggt gaccagtttt ttagcgcatc gggatcttta
     2581 cgcaccacgc cttcgccaga gcagggagcg tccagcaaaa tggcatcgaa catttctggc
     2641 actgccgcac caaacacgcg gccatcaaaa tgtgtgagcg caacattact gatgccacag
     2701 cggctgatat tggcatgtaa cacttttacc cgactggcgg aaaactcatt ggcaaggatt
     2761 gccccttcgt tattcatccg cgcggcaatt tgcgtcgttt tggagcctgg cgcagctgcg
     2821 acatccatca cccgctgtgg tgcattatcg tcagcaaaca aggcggcaac gggcaacatt
     2881 gaactggctt cctgaatata aaacaggccg cttaaatgct cggcggtact acccaatggc
     2941 aatgcatctt cattgtcgcg ttcaatccag aaaccttctt cacaccacgg aattggcgta
     3001 agcgtccagc cataaggagc ggttaattgc aggaaatcag caacggagat tttcagcgta
     3061 ttaacgcgaa tgctgcggcg caacgggcgc tgacaggcgg caagaaaatc atcaaatgag
     3121 agcgtcgaag gcatcgcttc gcgcatttgt gtcagaaagg cgtccgggaa ataaacggtg
     3181 tgttgggcca cgagcatgta ccactggcaa aaaaacaggc gcgcagttta gcataaacgc
     3241 tccggcgcag gcacgccgga gcagtgggca ttatttggga agcgcagttc cccattcacg
     3301 ccactctttc ggttcacttt cctgcaacag gaagtgtttg ccttcctggg ctttcggtgc
     3361 cagtggcgta cccggaggcg tggcgaaggc gatgccgcca cggataaact ggttaaaggt
     3421 gccggttttc actacgccgc ccgtcagacc aaagtccaga ctgtaacccg atgccaacca
     3481 gaagacggaa ttgttacgca ccaggtgttg atagcgttta ctgatgcgca tcgcaatcat
     3541 cacgcgatct gacaatgtcc ccagcgtcat tcctgtaacc gtaccgactt ccagaccacg
     3601 gaacagcaca ggcgtaccaa tacctaacga accggcttcc ggcgcttcaa caataatgct
     3661 taagccatcc aggtaacgcg aatcagtaat ggtggcctct tgtaattcaa agtcgcggcg
     3721 aggattgccc cggcctggtt cgacgttgat atacggctgg aggatagtat ctagatgctc
     3781 aacgcccgcg gctgaaattt gcggtgtgac caccgagaag cgcgtaccgc cgcgggcgaa
     3841 ggtctggaca tactccggat agagcaccgc ctttgcctgt acttcattgc gcgcggtaat
     3901 cagatccagc gtctggattt gcccgatatc aataccgaga tagcgaattg gcatcccgac
     3961 cgccagtttt ccggcatcga aagcgtgaag cgtaatctgc ccaccaaccg cacgggccgc
     4021 tgtttcggaa gcatacagaa ttcgtttgtc gcctttacgc tgactggcgc tggcaccgct
     4081 gaggttatcg aagctaatgg ctccctttaa tgctctggag agcggggatg cctgtacggt
     4141 caggccacta ccattcagct gaactttcgc cccgccttct gcccagaaca cgctattgct
     4201 ggtcagaagg ttgcgatact ccggcttaat atgcagatcg atatcaaacg cgttagctcg
     4261 cggacggacg gtaataactt caccaacttc aaatttacgg tagagcacta ccgatcctgc
     4321 ctgcacatcc ggcagcgtct ctgcactcaa actcacggtt gtggtgggta aatcgctaag
     4381 gctgttctcc agcgcttttt ccagattggc atacagtgga tagctggctt tcatctcgcc
     4441 tttatcgccc ggcagaatac gtatcccgcc gttaatccat tctgaggcgc tggcaccgag
     4501 aaactcaacg ccatccagcc ccaccttcac gtcgacacgg ctgttgacga caaatttgct
     4561 atcgcctttt accagttctc gatgctgagg ctcgatggcg acggtaaagg tgacgccttt
     4621 gctggtgagt ttacgatcga taacctggcc tacctgcacg ccgtgaagaa tgagcggctg
     4681 accagcatca ataccgtaac tttccggcgc ggtcagggtc agcgtcagaa catcaggttc
     4741 atgcagcagt gctttttcgc ctggcacaac aacgaactct ttgcgtggct cgccatcgcc
     4801 gggtaccaac tcgaaggttt tgccagtcag cagggcgctg agattggcat cgctaaggga
     4861 taatttcgga ttacgtaatt cgatgcgggt attttcacga agcagggtaa cgacgctggg
     4921 atcaactgtc atttccccag tgactttacc accaggattt aaatccagtt tagttagctg
     4981 tccgacttcc agcccctgat acattaacgg cgtcgagtcg gcggtcaatc cggccccact
     5041 cggcagttcc agttttatta ttacgccacg ctggctgtgg gccagatctt catacagacc
     5101 aaaggtatct tccgcctcgg caggtttcga ctcttcaggt gaatcgaagg caatcgcacc
     5161 gttaaccagt gccgccagac tttccagttt caccttcgcg ccactgacac tgacgttggc
     5221 atcaacgccg gaaacgttcc agaaacggct accttttttc accagatcgg taaaacgccg
     5281 ctcgatcaga acatcaatca ccacgccttg cttgttggga ttgatggcat agtcgtagac
     5341 ttttcccacc gggatcttgc ggaaatagac caatgaaccg ctgttcagcg aaccgagatc
     5401 gggagcttgc aggtggatca tcagatcgcc gttatccagc cgatacttcg gttgggtatc
     5461 gagtgcgaca aagtgatcct gctctttacc tttacccggc atcatgccga tatagttccc
     5521 accgacgagg gcgtccagcc cggagacacc tgccaacgat gcttttggcg tcaccagcca
     5581 gaattgagtc tcttcgcgca gcgcatcttt catatcggac ttgatgctga ccttgacttc
     5641 aatcttacga agatcgtcgc tgaggctgat atcctgcact gttccgactt caacgccctg
     5701 ataacgaaca ggcgtacggc ccggaacaat accatccgcc gacataaagt cgatggtgac
     5761 ggtattaccc cggtcctgat aactgtccca aatcagccaa ccggcaatca ttagcgcgat
     5821 gaaaggcagc agccagaaag gtgagatacg gcgtttattt ttaatctgcg cttcagtcgt
     5881 cgaagcgggc gtttcctgac tcatgtgcat cccaaagtaa gcggctgtcc agccattcca
     5941 cagcaagaat agtcaaaatt acggctgcgc caaaataaaa cgcagccggt cccatagtaa
     6001 aagcgaggat ctgatcgcga ttaatcagcg acatggttaa agatatgaca aacaggtcga
     6061 gcatcgacca gcgtccaatc caggtcacca tccgcagtaa cagaatgcgt gtgcgtaaac
     6121 cttgctggca tttaaaatga atgctcaaca gtaaagtgaa catgacgatc actttagtaa
     6181 acggtaccag aatactggcg ataaacacga ttcccgcaac cgcaatgttg ctacttgcca
     6241 gcgacataat tccggaaaga attgtatctt cctgccgtcc tccgttcaga taaatgatag
     6301 aaataggcaa taggttggct ggtaacaaca aaacgattga cgctaacagc gccgcccagc
     6361 atttttgcag actatgacgg cgacgcaggc gtagcgggat atggcaacgc gggcagcgac
     6421 cacgctgatc aggatagccg gtaaaatggc acccaagaca gacacgaagt ttctcgtccc
     6481 tacgcgtagc ggggcgctgc ggataatatc gctcccacag ctcctcaaca ttaagatgtg
     6541 acaacgtcac cgtcgttaaa atcaccaacg ccacaaaaga gaacaagccg acaccagcct
     6601 ggatatgggc ataatcctgt acctttatag aagcaacgcc aatgccgacc aggtagatat
     6661 ccagcattac ccactcttta agacgctcaa gcatcagcag taccggacgt aaattcattc
     6721 ccagtcggtt accaaaccat aaatatgcta tggaggacac cagaatgagg ggggcaccaa
     6781 taacgcagaa aaagaccatc gcccccgtta tcgtatcgcc ctgtttggtc atttgccaga
     6841 tgccttgcat gacgttggcg tcgatacgaa tacctaacag ccagatatgc aacagcggtt
     6901 cgccccaggc aaacggcatc agcaacagca tggtgaaagc cattgccgcc aggcgcgtta
     6961 gcgaccagtc gcgcccgtcg cgaatttttg cctgacagcg cggacaatag gcactctgat
     7021 gagaatttat ctcgggcagg ctaaacagca tgtcacattg cgggcaacgt tggtaatcac
     7081 cacgcggcag ttcctcgccg attgccctca ctgttatctt ttttgtcggc gtaatttgtg
     7141 gtgtgttaag agccatgacg tgccaaatgt aataagaatt aacattaata ttaacccatg
     7201 attggaatga tcttgagcat taacgcattt aatgcttatt ttaataggct ggcagttcca
     7261 ggtaagacaa ccaagtgatt atataatgaa caaaacagaa ttttacgcgg atttaaatcg
     7321 cgactttaac gcgctgatgg cgggagaaac cagttttctg gcaacgcttg cgaacaccag
     7381 tgcgttgtta tacgagcgtc tcactgacgt aaactgggca ggtttttatt tgcttgagga
     7441 cgatacactg gtactaggac catttcaggg caaaattgcc tgtgtccgga tacccgtcgg
     7501 gcgcggggtg tgcggcactg cggttgcccg caatcaagtg cagcgtatcg aggatgttca
     7561 cgcgtttgac gggcatattg cctgtgatgc ggcgagtaat tctgaaattg ttctgccgct
     7621 ggtggtgaaa aatcagatta ttggtgttct tgacatcgat agtaccgtct tcggtcgctt
     7681 tacagacgag gacgagcaag gcttacgtca gcttgtggca cagcttgaaa aagtgcttgc
     7741 aacgacggat tacaaaaaat tctttgcgag cgtcgcagga taatcaacgg ataacgtagc
     7801 atttactggt agcgtcatta taatgacgcc tgttcatgcc tgcgcttgtt ggctacgtcc
     7861 gttgtaatca ggaaatttca tggaaaatca acctaagttg aatagcagta aagaagtaat
     7921 cgcgtttctg gccgaacgtt ttccccactg tttcagtgcg gaaggtgaag cgcgtccgct
     7981 gaaaatcggt atttttcagg atttggtcga tcgtgttgcc ggggaaatga acctgagcaa
     8041 aacgcaatta cgatccgctt tacgtctcta cacttcgagc tggcgttatc tttacggtgt
     8101 taaacccggc gcaacgcgtg tcgatcttga cggcaaccca tgcggtgagc tggacgagca
     8161 acatgtagag catgctcgca agcagcttga agaagcgaaa gcgcgtgttc aggcacagcg
     8221 tgctgaacag caagcgaaaa aacgcgaagc tgccgcagct gctggtgaga aagaagacgc
     8281 accgcgccgc gaacgcaagc cacgtccgac cacgccacgc cgcaaagaag gcgctgaacg
     8341 taaacctcgt gcgcaaaagc cggtagagaa agcgccaaaa acagtaaaag cacctcgcga
     8401 agaacagcac accccagttt ctgacatttc agctctgact gtcggacaag ccctgaaggt
     8461 gaaagcgggt caaaacgcga tggatgccac cgtattagaa atcaccaaag acggcgtccg
     8521 cgtccagctg aattcgggta tgtctttgat tgtgcgcgca gaacacctgg tgttctgaaa
     8581 cggaggccgg gccaggcatg aacatgtttt ttaggcttac cgcgttagct ggcctgcttg
     8641 caatagcagg ccagaccttc gctgtagaag atatcacgcg tgctgatcaa attccggtat
     8701 taaaggaaga gacgcagcat gcgacggtaa gtgagcgcgt aacgtcgcgc ttcacccgtt
     8761 ctcattatcg ccagttcgac ctcgatcagg cattttcggc caaaatcttt gaccgctacc
     8821 tgaatctgct cgattacagc cacaacgtgc tgctggcaag cgatgttgaa cagttcgcga
     8881 aaaagaaaac cgagttaggc gatgaactgc gttcaggcaa actcgacgtt ttctacgatc
     8941 tctacaatct ggcgcaaaag cgccgttttg agcgttacca gtacgctttg tcggtactgg
     9001 aaaagccgat ggatttcacc ggcaacgaca cttataacct tgaccgcagc aaagcgccct
     9061 ggccgaaaaa cgaggctgag ttgaacgcgc tgtgggacag taaagtcaaa ttcgacgagt
     9121 taagcctgaa gctggcagga aaaacggata aagaaattcg tgaaaccctg acacgccgct
     9181 acaaatttgc cattcgtcgt ctggcgcaaa ccaacagcga agatgttttc tcgctggcaa
     9241 tgacggcgtt tgcgcgtgaa atcgacccgc ataccaacta tctttccccg cgtaataccg
     9301 aacagttcaa cactgaaatg agtttgtcgc tggaaggtat tggcgcagtg ctgcaaatgg
     9361 atgatgacta caccgttatc aattcgatgg tggcaggtgg tccggcagcg aagagtaaag
     9421 ctatcagcgt tggtgacaaa attgtcggtg ttggtcaaac aggcaagccg atggttgacg
     9481 tgattggctg gcgtcttgat gatgtggttg ccttaattaa agggccgaag ggcagtaaag
     9541 ttcgtctgga aattttacct gctggtaaag ggaccaagac ccgcactgta acattgaccc
     9601 gtgaacgtat tcgtctcgaa gaccgcgcgg ttaaaatgtc ggtgaagacc gtcggtaaag
     9661 agaaagtcgg cgtgctggat attccgggct tctatgtggg tttgacagac gatgtcaaag
     9721 ttcaactgca gaaactggaa aaacagaatg tcagcagtgt gatcatcgac ctgcgtagca
     9781 atggcggtgg ggcgttgacc gaagcggtat cgctctcagg tctgtttatt cctgcgggtc
     9841 ccattgttca ggtccgcgat aacaacggca aggttcgtga agacagcgat accgacgggc
     9901 aggtgttcta taaaggcccg ctggtggtac tggttgaccg tttcagtgct tcggcttcag
     9961 aaatctttgc cgcggcaatg caggattacg gtcgtgcgct ggttgtgggt gaaccgacgt
    10021 ttggtaaagg caccgttcag caataccgtt cattgaaccg tatttacgat cagatgttac
    10081 gtcctgaatg gccagcgctg ggttctgtgc agtacacgat ccagaaattc tatcgcgtta
    10141 acggcggcag tacgcaacgt aaaggcgtaa cgccagacat catcatgccg acgggtaatg
    10201 aagaaacgga aacgggtgag aaattcgaag ataacgcgct gccgtgggat agcattgatg
    10261 ccgcgactta tgtgaaatca ggcgatttaa cggcctttgg gccggagctg ctgaaggaac
    10321 ataatgcgcg tatcgcgaaa gatcctgagt tccagaacat tatgaaggat atcgcgcgct
    10381 tcaacgctat gaaggacaag cgcaatatcg tttctctgaa ctacgctgtg cgtgagaaag
    10441 agaataatga agatgatgcg acgcgtctgg cgcgtttgaa cgaacgcttt aaacgcgaag
    10501 gtaaaccgga gttgaagaag ctggatgatc taccgaaaga ttaccaggag ccggatcctt
    10561 atctggatga gacggtgaat atcgcactcg atctggcgaa gcttgaaaaa gccagacccg
    10621 cggaacaacc cgatcccgtc aagtaatatc aatcaggcac aagaaattgt gcctgatttt
    10681 ttaacagcgg caagatgccg taaatcagat gctacaaaat gtaaagttgt gtctttctgg
    10741 tgacttacgc actatccaga cttgaaaata gtcgcgtaac ccatacgatg tgggtatcgc
    10801 atattgcgtt ttgttaaact gaggtaaaaa gaaaattatg atgcgaatcg cgctcttcct
    10861 gctaacgaac ctggccgtaa tggtcgtttt cgggctggta ctgagcctga cagggataca
    10921 gtcgagcagc gttcaggggc tgatgatcat ggccttgctg ttcggttttg gtggttcctt
    10981 cgtttcgctt ctgatgtcca aatggatggc attacgatct gttggcgggg aagtgatcga
    11041 gcaaccgcgt aacgaaaggg aacgttggct ggtcaatact gtagcaaccc aggctcgtca
    11101 ggcagggatc gctatgccgc aagtggctat ctaccatgcg ccggacatca acgcttttgc
    11161 aaccggtgcg cgccgtgatg cctctctggt tgctgtcagc accggtttgc tgcagaacat
    11221 gagcccggat gaagccgagg cggtaattgc tcacgaaatc agtcacatcg ccaatggtga
    11281 tatggttacc atgacgctga ttcagggcgt ggtgaacacc ttcgttatct ttatttcccg
    11341 tattctggcg cagcttgccg cgggttttat gggcggaaat cgtgatgaag gtgaagagag
    11401 caacggcaac ccgctgatct actttgcggt tgcaacggtt ctggaactgg tgtttggtat
    11461 tctggcgagc attatcacca tgtggttctc gcgtcatcgt gaattccatg ctgatgccgg
    11521 ttcggcaaaa ctggttggtc gcgagaaaat gattgccgcg ctgcagcgcc tgaaaaccag
    11581 ctatgaaccg caagaagcaa ccagcatgat ggctttctgc attaacggta agtcgaaatc
    11641 gctcagtgag ttgttcatga ctcacccgcc gctggataaa cgaattgaag ctctgcgtac
    11701 gggtgaatac ctgaagtaat atgaagtaaa tactaaaagg cgcgtcctga tcgacgcgct
    11761 ttttttatgc cctggatcgt ggctgagtga tacgtaaacc actcacacag gcagcaatca
    11821 ctgccagaat agccgcagcc atcagcgaaa cgtgcgtacc attatcaccg aactgattta
    11881 gcatcagcgc caccagcgcc gcgccactac tctgacccag tagacgagcc gttcctaaca
    11941 tgccactggc cccaccgcta cgttcgcgcg gagcggaggt aataatggtg tggttattgg
    12001 gtgactggaa taagccaaat ccagcgccac ataagatcat cggccagata atattgatat
    12061 ccgcaggtga cgcgggcagc agaaccaggg aaaaaagccc cgcagccatg atgaacaacc
    12121 ctaaagcacc cagcaatcct gcatgtacgc gttcaatcaa atagcctgcc agcggagcca
    12181 tcaccatcgt tgctaacggc cacggtttca gaagtaaacc tgtttcgact tcactacgcc
    12241 cgagcacggt ttgcaggtaa aagggcaggg aaaccattgc cagcatttgt gcgcagaaag
    12301 agcaaacaga tgtgcaaata gaaagtgaaa acagcgggat acgcagtaaa tccaccggta
    12361 gcagcggtac gggaagagaa agctggcggc gaataaagaa aataccaaca acaaccatta
    12421 ccaccagttc cgcagcaatt aacgtcagcg attgcccctg agcgaaacca ctcaacgcag
    12481 tgataagcag gccgaaggtt aacgcgttca tcacggcgct gggcaggtcg aaacggggtt
    12541 tactggcgcg agaaccattg ggtggcagaa aacgcatcgc cagaagcagg gcgataatac
    12601 ctaacggtac gttgattaaa aataaccatt tccaggatgc gatggagagg attgctgcag
    12661 caattgtcgg cccggcagca gaagaaacgg caacaataaa cgagtttatg cccatccctc
    12721 tccccagaaa acgttgtgga tagatcaggc ggataagtgc ggtattaacg ctcatcaacg
    12781 ctgcaccgcc gaaaccttgt atgacacgcg caagggtgag catttgcagc gaatcagaaa
    12841 gggcgcagaa cagtgaagac aacagaaaaa cgaccagacc gcatttataa atacgtcgat
    12901 agccaaacat atcgcccaga aacgaaaacg agagcaggga gatgacaatg gcgatttgat
    12961 aggcgttcac tacccagatg gatctggctg gcgtggcatg aaggtccgtg gcgattgttg
    13021 gcagggcgac gttggcgatt gcgccgtcaa ggacagccat cgaaatgcca atcacaatgg
    13081 ttaatatcgc accgtatcgc tggggcaatg gcaggccgtc ggcctgaact tttggcatag
    13141 gaattttata tctttggtga ataatcaaca ggggtatgat tctagcactg atattcacgg
    13201 tggatgtcgc agaattgtaa ctaaccggca aaagatggat tgcgaccacc ctggtgcgaa
    13261 attataataa aaaacagttc tgatttttat aaaacactcg caatgaggtg ataaatggct
    13321 aacgcagatc tggataaaca gcctgattct gtatcttccg tgctaaaagt ttttggcatt
    13381 ttgcaggcgc tgggtgaaga gcgcgaaata gggataaccg agctgtcgca gcgcgtcatg
    13441 atgtcaaaaa gcaccgttta tcgcttttta cagaccatga aaaccttagg ttatgtggcg
    13501 caggaagggg agtcggagaa atattccctg accctgaaat tgtttgaact gggcgctcgc
    13561 gcgttacaaa acgtcgattt aattcgtagc gcagatatcc agatgcgtga gatctcccgc
    13621 ctgaccaaag aaactatcca cctcggcgca ctggacgaag acagtattgt ttacattcac
    13681 aaaattgact ctatgtacaa tttgcgcatg tattcacgga ttgggcgtcg taatccgctg
    13741 tacagcaccg cgattggtaa ggtactgctg gcatggcgcg atctcgatga agtgaagcaa
    13801 attcttgagg gcgtggagta taaacgcagt accgagcgga ccatcaccag tacagaagcg
    13861 ttattacccg ttctggacca ggtgcgcgag caggggtatg gcgaagataa cgaagagcag
    13921 gaagaagggc tgcgatgcat tgcggtaccg gtatttgatc gctttggcgt ggtcattgcc
    13981 ggtttgagca tctccttccc gacgttgcgt ttctctgaag agcgtttaca ggaatatgtc
    14041 gcaatgttgc ataccgcagc gcgcaaaatt tctgcccaaa tgggttatca cgactatccg
    14101 ttctgatgag agtaagaacc tgtcggaata tcaaacagac aggttcttta tttagcatga
    14161 gaaaaataaa gttgaaggtg cgttatatta aacgcgcttg ctataagaat attttactca
    14221 tgagtgagaa tctggttatt tattaccctt aaccattatc gaccacgata ttgcttttac
    14281 gtaacagtgg gcaatctgtt atccccaaga aaccactttt agtgtgcaag tattgtaccg
    14341 tgctggtgcc tctggcagtc agataggtac attgcaaacc taatcctgcg gcattctctt
    14401 tgcttccaat caaaacgcca tatccgctga gtaataatcc tatccatacc agtgctatca
    14461 gcataactgt gcgaatgatg aatcgcatta caacctcttc tctttttatg ttcgcttaat
    14521 cgtagcggca atatgcgctg aagcaagcta ctcattccga aaaagcacga atatcgacat
    14581 agttaggcgc tgtttaacta acgcatgcta gtttaatgac ataaggtagg tgaagcggag
    14641 attggagtga aaaagtttcg atgggtcgtt ctggttgtcg tggtgttggc ttgcttgctg
    14701 ctttgggcgc aggtattcaa catgatgtgc gatcaggatg tacaattttt cagcggaatt
    14761 tgtgccatta accagtttat cccgtggtga tatcattttt tcagcactag attttcttcc
    14821 tctccagcag tggtagaata gcgcctctac tcagaggtgg tgaaatgaac gaagttgtaa
    14881 attcaggcgt gatgaacatt gcgtctttgg ttgtatcggt ggtggttctt cttatcgggc
    14941 tcatcttgtg gttttttatc aatcgtgcca gttctcggac taacgaacag attgaactgc
    15001 ttgaggcgtt gctggatcag caaaaacgtc aaaatgcact gttacgtcgt ttgtgcgaag
    15061 caaacgaacc tgagaaagca gataaaaaga ccgttgagag tcaaaaatcg gttgaagacg
    15121 aagatattat tcgtctggtc gccgaacgat aatccttcct caatcttgag taaccccgtc
    15181 gttgaagggt tactcaattc atcctgatta tacgcactgc catggtgcaa ctgcttcaaa
    15241 ttaaa
//
LOCUS       DAZGLJ010000109        15227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000109 DAZGLJ010000000
VERSION     DAZGLJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.109"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            20..1309
                     /gene="hpaG"
                     /locus_tag="ACHK49_002912"
     CDS             20..1309
                     /gene="hpaG"
                     /locus_tag="ACHK49_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFO9263529.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGCLVNRIVSEETAK"
     gene            1306..2772
                     /gene="hpaE"
                     /locus_tag="ACHK49_002913"
     CDS             1306..2772
                     /gene="hpaE"
                     /locus_tag="ACHK49_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFO9263530.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2774..3625
                     /gene="hpaD"
                     /locus_tag="ACHK49_002914"
     CDS             2774..3625
                     /gene="hpaD"
                     /locus_tag="ACHK49_002914"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFO9263531.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3635..4015
                     /locus_tag="ACHK49_002915"
     CDS             3635..4015
                     /locus_tag="ACHK49_002915"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFO9263532.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQHDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4126..4929
                     /gene="hpaH"
                     /locus_tag="ACHK49_002916"
     CDS             4126..4929
                     /gene="hpaH"
                     /locus_tag="ACHK49_002916"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFO9263533.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4940..5728
                     /gene="hpaI"
                     /locus_tag="ACHK49_002917"
     CDS             4940..5728
                     /gene="hpaI"
                     /locus_tag="ACHK49_002917"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFO9263534.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAIKPDVY"
     gene            5903..7279
                     /gene="hpaX"
                     /locus_tag="ACHK49_002918"
     CDS             5903..7279
                     /gene="hpaX"
                     /locus_tag="ACHK49_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFO9263535.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMIYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVATLLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7289..8179
                     /gene="hpaA"
                     /locus_tag="ACHK49_002919"
     CDS             7289..8179
                     /gene="hpaA"
                     /locus_tag="ACHK49_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFO9263536.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFHMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8430..9992
                     /gene="hpaB"
                     /locus_tag="ACHK49_002920"
     CDS             8430..9992
                     /gene="hpaB"
                     /locus_tag="ACHK49_002920"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFO9263537.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10010..10522
                     /locus_tag="ACHK49_002921"
     CDS             10010..10522
                     /locus_tag="ACHK49_002921"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFO9263538.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSIT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10915..13065
                     /gene="btsT"
                     /locus_tag="ACHK49_002922"
     CDS             10915..13065
                     /gene="btsT"
                     /locus_tag="ACHK49_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO9263539.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13196..13399
                     /locus_tag="ACHK49_002923"
     CDS             13196..13399
                     /locus_tag="ACHK49_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO9263540.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13410..14366
                     /gene="yjiA"
                     /locus_tag="ACHK49_002924"
     CDS             13410..14366
                     /gene="yjiA"
                     /locus_tag="ACHK49_002924"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO9263541.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(14453..14644)
                     /locus_tag="ACHK49_002925"
     CDS             complement(14453..14644)
                     /locus_tag="ACHK49_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFO9263542.1"
                     /translation="MSMVKHKRGTLYTLDVQQEAELMALVNKSDEDIDYSDIPASGSE
                     AWSDAKRGNFLPDPGIHAQ"
     gene            complement(14684..>15227)
                     /locus_tag="ACHK49_002926"
     CDS             complement(14684..>15227)
                     /locus_tag="ACHK49_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263543.1"
                     /translation="RSMIIACIPIIYLFASSCASSLFLSLSNLNITLTPWVEYFWKGM
                     AFMLLFFMLMQLVIYFAFLTLWAKLSVYRLFILAGAFIVSTILVAFASKNVEGISYYV
                     LKSTIDFEWRNQVKCGELNISRPDERYFGFNTDKYTVFYSNREGKWGVNELKCKKGSD
                     RRDAYSIENVSEYNVPGWLK"
BASE COUNT         3582 a         4159 c         4121 g         3365 t
ORIGIN      
        1 tcgaatccga ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag
       61 ccaacttgat gcatggcagg aagcgttcca gcaatccccc tacaaagccc cgcctaaaac
      121 tgcggtctgg tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt
      181 tccacagggt gaaaaggtac tgagcggtgc gactgttgcg ctgattgtgg gaaaaacggc
      241 gacgaaagta cgtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggctaacga
      301 cgtcagcctg ccggaagaga gcttttaccg cccggcaatc aaagcaaaat gtcgtgatgg
      361 attctgcccc attggcgaaa ccgtggctct cagcaatgtc gataatctga ccatctatac
      421 cgagatcaac gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc
      481 gcagttgctg agcgccctga gcgaatttgc cacactgaat ccaggcgatg ccattctgct
      541 cggcacgcca caggcgcgcg tggaaataca gccaggcgat cgcgttcgtg ttctcgcaga
      601 aggtttcccg ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcgcaagag
      661 cttcccaacg cagccacacc cgcacggcac gctgtttgcc ctcggcctga actacgccga
      721 ccacgccagc gaactggaat ttaagccacc ggaagaaccg ctggtgttcc tgaaagcgcc
      781 aaataccctc actggcgata accagacctc cgtgcgtcca aacaatattg aatacatgca
      841 ctatgaagcg gagctggtgg tagttattgg caaacaggcg cgtaacgtca gcgaagccga
      901 tgccatggat tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gcgactatct
      961 ggaaaactac taccgcccta acctgcgggt aaaaagccgc gacggactga cgccgatgct
     1021 ttcaaccatc gtgccgaaag aggcgatccc ggacccgcat aatctgaccc ttcgcacctt
     1081 cgtcaacggc gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt
     1141 cctgatcgcc tacttaagcg aatttatgac cctgaatccg ggcgacatga tcgccaccgg
     1201 cacaccaaaa ggcttatctg acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg
     1261 cgtgggctgc ctggtgaacc gaattgtgag tgaggaaaca gcgaaatgaa aaaagtaaat
     1321 cattggatca acggcaaaaa tgttgcaggt aacgactact tccagaccac caatccggca
     1381 acgggtgaag tgctggcgga tgtggcctct ggcggtgaag cggagatcaa tcaggcggta
     1441 gcggcagcga aagaggcgtt cccgaaatgg gccaatctgc cgatgaaaga gcgtgcgcgc
     1501 ctgatgcgcc gtctgggcga tctgatcgac cagaacgtgc cagagatcgc cgcgatggaa
     1561 accgcggaca ccggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttcc
     1621 cacaactttg aatttttcgc ggaagtctgc cagcagatga acggcaagac ctatccggtt
     1681 gacgacaaga tgctcaacta cacgctggtg cagccggtgg gcgtttgtgc gctggtatcg
     1741 ccgtggaacg taccgtttat gaccgccaca tggaaggtcg cgccgtgtct ggcgctgggc
     1801 aatactgcgg tgctgaagat gtcagaactc tccccgctga ccgctgaccg cctgggtgag
     1861 ctggcgctgg aagccggtat tccggcgggc gtgctgaacg tggtacaggg ctacggcgca
     1921 accgcagggg atgcgctggt ccgtcatcat gacgtgcgtg ccgtgtcgtt caccggcggt
     1981 accgccaccg ggcgcaacat catgaaaaac gccgggctga aaaaatactc catggaactg
     2041 ggcggaaaat cgccagtgct gatttttgaa gatgccgata ttgaacgcgc gctggacgcc
     2101 gccctgttca ccatcttctc gatcaacggc gagcgctgca ccgccggttc gcgcatcttt
     2161 attcagcaaa gcatctaccc ggaattcgtt aaacgctttg ccgaacgcgc caaccgtctg
     2221 cgcgtgggcg atccgaacga tccgaatacc caggttgggg cacttatcag ccagcaacac
     2281 tgggaaaaag tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgctggcg
     2341 ggcggcccgg ataaaccgtc tgacctgcct gcgcacctga aaggcggcaa cttcctgcgc
     2401 ccaacggtgc tggcagacgt agataaccgt atgcgcgtcg cccaggaaga gattttcggg
     2461 ccggtcgcct gcctgctgcc gtttaaagac gaagccgaag gcttacgcct ggcaaacgac
     2521 gtggagtacg gcctcgcgtc gtacatctgg acacaggatg tcagcaaagt gttacgcctg
     2581 gcgcgtggca ttgaagcagg catggtgttc gtcaataccc agaacgtccg cgacctgcgc
     2641 cagccgtttg gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc
     2701 gaagtgttcg cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa
     2761 tggggagtct gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcgat
     2821 gtatctctct gaactaccag gaaaaaacca cggttgtcgc cagggtgcga tcgacgggca
     2881 taaagagatc agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgacac
     2941 ccactggctg gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcgtcta
     3001 caccagtaac gaactgccgc attttattcg tgacatgacc tacaactacg agggcaaccc
     3061 ggagttgggg cagctaattg ccgatgaagc cttaaagctc ggcgtgcggg caaaagcgca
     3121 caatattccc agcctgaaac tggaatacgg cacgctggtg ccgatgcgct acatgaatga
     3181 agacaagcac ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga
     3241 cagccgcaag ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc
     3301 ggtccttgcc agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cggaagaagg
     3361 gatgaacagc tacacccgcg agttcgaccg ccagatggac gagcgcgtgg tgaagctgtg
     3421 gcgcgaaggc cagttcaaag agttttgcaa tatgctgccg gagtacgccg actactgcta
     3481 cggcgaaggc aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata
     3541 cgacggcaag gtggagttta tcaccgagct attcccaagc tctggcaccg gtcaggttaa
     3601 cgctgttttc ccgcttcccg cgtaaggacg ttttatgccg cactttatcg ttgaatgcag
     3661 tgataacatc cgcgaagaag ccgacctgcc ggggttgttc gccaaagtga atccgacgct
     3721 ggcagccacg ggtatttttc cgctggcggg tattcgcagc cgcgtgcatt gggtcgatac
     3781 ctggcagatg gccgacgggc agcatgatta tgccttcgtg catatgacgt tgaaaatcgg
     3841 cgcaggtcgc agcctggaaa gccgccagca ggcgggtgaa atgctgtttg aactgattaa
     3901 aacgcacttc gccgccctga tggagagccg cctgctggcg ttgtcgtttg agattgaaga
     3961 gctgcatccg acgctgaatt ttaaacaaaa caacgtgcac gcattgttta agtagcgcgc
     4021 agattgcccg gtggcgctgc gcttaccggg cctacaaaac cccaaaccgt acaccgtagg
     4081 ccggataagg cgcagccgca tccggcaatg ccacaggata tcgctatgtt cgataaacac
     4141 acccacaccc tgatcgccca gcgtctggat caggcagaaa aacagcgcga acagatccgc
     4201 gcgatctcgc tggattaccc ggagatcacc atcgaagacg cttacgcggt gcagcgtgaa
     4261 tgggttcgcc tgaaaatcgc cgaaggtcgc acgctgaaag gccacaaaat cggcctgacc
     4321 tcgaaagcga tgcaggcaag ctcgcagatc agcgaaccgg attacggcgc gctgctggat
     4381 gacatgttct tccacgatgg cagcgatatc ccgaccgatc gctttatcgt gccgcgcatt
     4441 gaagtggagc tggctttcgt gctggcaaaa ccgctgcgtg ggccaaactg cacgctgttc
     4501 gacgtctaca acgccacgga ctacgtgatc ccggcgctgg agctgatcga cgctcgctgc
     4561 cacaacatcg atccggaaac ccagcgcccg cgcaaagttt tcgacaccat ttctgataac
     4621 gccgccaacg ccggggtgat cctcggaggt cgtccgatca aaccagacga actggatctg
     4681 cgttggatct ccgccctgat gtatcgcaat ggcgtgattg aagaaaccgg cgtcgccgct
     4741 ggcgtgctga atcatccggc aaacggcgtg gcctggctgg cgaacaaact cgcaccgtat
     4801 gacgtacaac tggaagccgg gcaaatcatt ctcggtggtt cgttcacccg cccggttccg
     4861 gcgcgtaagg gcgacacctt ccacgtcgat tacggcaaca tgggttccat tagctgccgc
     4921 tttgtttaag gagagaacga tggaaaacag ctttaaagcg gcgctgaaag caggccgtcc
     4981 gcagatcgga ttatggctgg ggctgagtag cagctacagc gcagagttac tggccggagc
     5041 aggattcgac tggttgttga tcgacggtga gcacgcgccg aataacgtgc aaaccgtgct
     5101 cacccagcta caggcgattg cgccctaccc cagccagccg gtagtacgtc cgtcgtggaa
     5161 cgatccggtg caaatcaaac aactgctgga cgtcggcaca caaaccttgc tggtgccgat
     5221 ggtacaaaac gctgacgaag cccgtgaagc ggtacgcgcc acccgttatc cccccgccgg
     5281 tattcgcggt gtgggcagtg cgctggcccg agcctcgcgc tggaatcgca ttcctgatta
     5341 cctgcaaaaa gccaacgatc aaatgtgcgt gctggtgcag atcgaaacgc gtgaggcaat
     5401 gaagaactta ccgcagattc tggacgtgga aggggtcgac ggcgtgttta tcggcccggc
     5461 ggatctgagc gccgatatgg gttatgccgg taatcctcag cacccggaag tacaggccgc
     5521 gattgagcag gcgatcgtgc agatccgcga agcgggcaaa gcgccgggga tcctgatcgc
     5581 caatgagcaa ctggcaaaac gctatctgga actgggcgcg ctgtttgtcg ccgtcggcgt
     5641 tgacaccacc ctgctcgccc gcgccgccga agcgctggca gcacggtttg gtgcgcaggc
     5701 tacagcgatt aagcccgacg tgtattaatg ccggatgcgc tgcgcttatc cggcctacac
     5761 tcgcaccaaa ccgtaggccg gataaggcgt ttacgccgca tccggcaaaa agccgtacca
     5821 aatcgcggac cggataaggc gtttgtgcgc atccgccata aaccttgtcg taccctacaa
     5881 aaatcccatt agaggaagta aaatgagcga cacctcacct gccataccgg agagtatcga
     5941 tccggcgaat cagcataaag cgctgactgc cggacaacag gcggttatta agaagctatt
     6001 tcgccgcctg atcgtctttc tgttcgtgct gtttatcttc tcgttccttg atcgcatcaa
     6061 catcggcttt gccggactca cgatgggacg cgacctcggt ctgagcgcca ccatgtttgg
     6121 cctcgctacc accttgttct acgccgctta cgtcatcttc ggcattccca gcaacattat
     6181 gctgagtatt gtcggtgcac ggcgctggat cgccaccatc atggtgcttt ggggcatcgc
     6241 ctctactgcc accatgtttg ccactggccc caccagctta tacgtactgc gtatactggt
     6301 tggcattacc gaagccggct ttctgcctgg cattctgctg tatttaacct tctggtttcc
     6361 ggcctatttc cgcgcccgtg ccaacgcctt gtttatggta gcaatgccgg taacgacagc
     6421 gttgggatcg atcgtttccg gctacatttt gtcgctggat ggcgtaatgg cattaaaagg
     6481 ctggcagtgg ctgtttttgc tggaaggctt cccgtcggta ttactcggcg tcatggtgtg
     6541 gttctggctt gatgactcac cggacaaagc taagtggctg acgaaagaag acaaaaaatg
     6601 cctgcaagag atgatggata acgatcgtct gacgctggtt cagccagagg gagccatcag
     6661 ccaccacgcc atgcaacaac gcagtatgtg gcgggagatc ttcactccgg tggtgatgat
     6721 gtataccctg gcgtatttct gcctgaccaa cacacttagt gcgatcagca tctggacacc
     6781 gcagatcctg caaagcttta atcagggcag cagtaatatc accatcggcc tgctggccgc
     6841 cgtaccgcag atttgtacca ttctcgggat gatctactgg agccgtcact cagatcgccg
     6901 ccaggaacga aggcatcaca ccgcccttcc ttatttgttc gctgccgctg gttggttact
     6961 ggcttcggca actgatcaca acatgatcca gatgctgggg atcattatgg cttcgaccgg
     7021 atcattcagc gcaatggcga ttttctggac aacaccggat cagtccatca gcctgcgggc
     7081 acgagcgatc ggtattgcgg tgatcaacgc cactggcaac attggttcag cgttaagtcc
     7141 gtttatgatc ggctggttga aagatctgac cggcagcttt aacagtggat tgtggtttgt
     7201 tgccacgctg ctggtgattg gtgcggggat tatctgggcg attccaatgc agtcctcccg
     7261 tccgcgagcg accccgtaag gaacgacgat gtgtgaccgt cagattgcca atattgatat
     7321 cagcaaagag tacgatgaaa gcctgggcac ggacgatgtg cattatcagt cgttcgcccg
     7381 catggcggcc ttttttggcc gccatatgct gccacatcgc cacgaacagt actttcagat
     7441 gcatttcctc aatagcggac agattgagct acagcttgac gatcatcgct actcggtgga
     7501 agcgcccctg tttgtcctga cgccgccgtc agtacctcat gcgtttatta cggagtctga
     7561 tgctgacggt catgtattga cggtacggga agatctgatc tggcccctgc tggaagttct
     7621 ttatccgggc actcgggaaa ccttcggcct gccggggatt tgcctgtcac tggcagataa
     7681 acccgacgaa ctggcggcgc tggaacacta ttggcaactg atagagcggg aatcggtaga
     7741 acaactgcct ggtcgggaac acaccctgac gttactggca caggcagtgt tcaccctact
     7801 gctgcgtaac gcaaaactcg acgaccatgc cgccagcgga atgcgcggag aattaaaact
     7861 gttccagcgt tttcatatgc tgattgaaag ccattttcat cagcactgga cagtaccgga
     7921 ttacgctaac gaattgcata tcaccgaatc acgcctcact gacatctgcc gccgctttgc
     7981 caaccgtccg ccaaaacggt tgattttcga caggcagctg cgagaagcca agcggctgct
     8041 gctgttttct gataacgccg tgaacaatat tgcctggcaa ctcggtttta aggatccggc
     8101 ttattttgcg cgctttttta atcgcttagt cggttgctcg cccagtgctt atcgcgccaa
     8161 aaaagtacct gtgacgtgaa aatcccttag ccttaacggg aaaccaggca ccacctgcta
     8221 ttcccctttc tccgcctgga gcaaggggat ttagtaacgt taaaaatcgc taaaagcgac
     8281 ctcgatcaca aaaccgtacc cgatctgaaa agtaccagcc attgtcccaa aagtgtcttc
     8341 ccgtaatcgc ccctgcaagc ttcaattgaa taacaaaaac aacacacaaa attaacaatc
     8401 gtattccgat taatactgta gaggtcgaca tgaaaccaga agatttccgc gccagtaccc
     8461 aacgtccgtt caccggggaa gagtatctga aaagcctgca ggatggtcgc gagatctata
     8521 tctatggcga gcgagtgaaa gacgtcacca ctcatccggc atttcgtaat gcggcagcgt
     8581 ctgttgccca actgtacgac gcgctacaca aaccggagat gcaggactct ctgtgctgga
     8641 acaccgacac cggtagcggc ggctataccc ataaattctt ccgcgtggcg aaaagtgccg
     8701 acgacctgcg ccagcaacgc gacgccatcg ctgagtggtc acgcctgagc tatggctgga
     8761 tgggccgtac cccagactac aaagccgctt tcggttgcgc actgggcgcg aatccgggct
     8821 tttacggtca gttcgagcag aacgcccgta actggtatac ccgtattcag gaaactggcc
     8881 tctactttaa ccacgcgatt gttaacccac cgatcgatcg tcatttgccg accgataaag
     8941 tgaaagacgt ttacatcaag ctggaaaaag agactgacgc cgggattatc gtcagcggtg
     9001 cgaaagtggt tgccaccaac tcggcgctga ctcactacaa catgattggc ttcggctcgg
     9061 cacaagtgat gggcgaaaac ccggacttcg cactgatgtt cgttgcgcca atggatgccg
     9121 atggcgtgaa attaatctcc cgcgcctctt atgagatggt cgcgggtgct accggctcgc
     9181 cgtacgacta cccgctctcc agccgctttg atgagaatga tgcgattctg gtgatggata
     9241 acgtgctgat cccatgggaa aacgtactga tctaccgcga tttcgatcgc tgccgtcgct
     9301 ggacgatgga aggcggtttt gcccgtatgt atccgctgca agcctgtgtg cgcctggcag
     9361 tgaaactaga cttcattacg gcactgctga aaaaatcact cgaatgtacc ggcaccctgg
     9421 agttccgtgg tgtgcaggcc gatctcggtg aagtggtggc gtggcgcaac accttctggg
     9481 cattgagtga ctcgatgtgt tcagaagcga caccatgggt caacggtgct tatttaccgg
     9541 atcatgccgc actgcaaacc tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa
     9601 acattatcga acgcaacgtt accagtggcc tgatctatct cccttccagt gcccgtgacc
     9661 tgaacaatcc gcagatcgac cagtatctgg cgaagtatgt gcgcggttcg aacggtatgg
     9721 atcacgtcca gcgcatcaag atcctcaaac tgatgtggga cgccattggc agcgagtttg
     9781 gtggtcgtca cgaactgtat gaaatcaact actccggtag ccaggatgag attcgcctgc
     9841 agtgtctgcg ccaggcacaa agctccggca atatggacaa gatgatggcg atggttgatc
     9901 gctgcctgtc ggaatacgac cagaacggct ggactgtgcc gcacctgcac aacaacgacg
     9961 atatcaacat gctggataag ctgctgaaat aacgcagcag gaggttaaga tgcaattaga
    10021 tgaacaacgc ctgcgctttc gtgacgcaat ggccagcctg tcggcagcgg taaatattat
    10081 caccaccgag ggcgacgccg gacaatgcgg aattacggca acggccgtct gctcgatcac
    10141 ggatacacca ccgtcgctga tggtgtgcat taacgccaac agtgcgatga acccggtttt
    10201 tcagggcaac ggtaagttgt gcgtcaacgt cctcaatcat gagcaggagc tgatggcccg
    10261 ccacttcgcg ggcatgacag gcatggcgat ggaagagcgt tttagcctct catgctggca
    10321 aaaaggtccg ctggcacagc cggtgctgaa aggttcgctg gccagtcttg aaggtgagat
    10381 ccgcgatgtg caggcaattg gcacacatct ggtgtatctg gtggagatta aaaacatcat
    10441 cctcagtgca gaaggtcacg gacttatcta ctttaaacgc cgtttccatc cggtgatgct
    10501 ggaaatggaa gctgcgattt aagctccagc cccttcacgc attcgtcgaa ggggcatttt
    10561 tattgtgatc tcaatcacat atccacccga tgaataattc ttacccaacc gctcaccgcc
    10621 attcctgacc gcttacatcc ctaaaataac cactcagtta tttaccttac tttacgcgtg
    10681 cgtaactctg gcaacatcac tgcaggatag cggtcaattt acctcctcaa acgcaacgca
    10741 aacctagaac ggcttcggcc tatgattaac aaattactct atggattttg gtttgcagga
    10801 aggcagcaag tgagtgaatc cccgggagct tataacagta agtgacaggg gtgaacgaac
    10861 gcagctgccg cacctgtaag ccaaaagacg acgagtaaaa tgccaggttt tactatggat
    10921 acgaaaaaac tattcaagca cataccctgg gtgattctcg gaatcatcgg tgcattctgc
    10981 ctcgcggtag ttgcattacg tcggggggag cacgtcagcg ccctgtggat cgtggtcgcc
    11041 tctgtgtcgg tgtatctggt ggcgtatcgc tactacagtc tgtatatcgc ccagaaggtg
    11101 atgaaactcg accccacgcg cgcgacgcct gcggttatta acaacgacgg tctgaactac
    11161 gttccgacca accgttacgt gttgtttggt caccacttcg ccgctatcgc cggtgctggt
    11221 ccgctggttg gtccggttct cgccgcgcag atgggctacc tgcccggcac gctgtggctg
    11281 ctggcggggg tagtgctggc cggtgcggta caggacttta tggtgctgtt tatctcctct
    11341 cgccgtaacg gcgcatctct cggtgagatg atcaaagaag agatgggacc agtaccgggg
    11401 actatcgcgc tgtttggctg tttcttaatc atgatcatca tccttgccgt cctggcgctg
    11461 attgtggtta aagccctggc agaaagtccg tggggtgtct tcaccgtttg ctcaaccgta
    11521 ccgattgcgc tgtttatggg tatctacatg cgctttatcc gtccggggcg tgtgggtgaa
    11581 gtctctgtaa tcggtatcgt gctgctggtt gcctctatct acttcggtgg cgtgattgct
    11641 cacgatccgt actggggtcc ggcactgacc tttaaagaca ccaccattac cttcgcgctg
    11701 attggctatg cgtttgtttc cgcactgctg ccggtgtggc tgatcctcgc accgcgcgac
    11761 tatctggcaa ccttcctgaa aatcggcgtt atcgtcggcc tggcgttggg tatcgtggtg
    11821 ctgaacccgg aactgaaaat gcctgcgatg acccagtaca ttgacggtac tggcccgctg
    11881 tggaaaggcg ctctgttccc gttcctgttc atcaccatcg cctgtggtgc ggtatctggc
    11941 ttccacgcgc tgatctcttc cggtacgacg ccgaaactgc tggctaacga aaccgacgcg
    12001 cgtttcattg gctacggcgc aatgctgatg gagtccttcg tggcgattat ggcgctggtt
    12061 gcagcgtcca tcatcgaacc gggtctttac ttcgcgatga acaccccgcc tgcgggcctt
    12121 ggcatcacca tgcctaacct gcatgaaatg ggtggcgaga acgcgccgat catcatggcg
    12181 cagctgaaag acgttaccgc acacgcggcg gcgaccgtca gctcctgggg cttcgtgatt
    12241 tcgccagagc agatcctgca aaccgcgaaa gacattggtg agccttctgt cctgaaccgt
    12301 gcaggtggtg cgccgacact ggcggtaggt atcgcgcacg tgttccacaa agtgctgccg
    12361 atggctgaca tgggcttctg gtatcacttc ggtattctgt tcgaagccct gttcatcctg
    12421 accgcgctgg atgcgggtac ccgttctggc cgctttatgc tgcaggacct gctgggtaac
    12481 ttcatcccgt tcctgaaaaa aaccgactct ctggttgctg gtatcatcgg tactgcgggc
    12541 tgtgtgggtc tgtggggcta cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt
    12601 aagagcctgt ggccgctgtt cggtatctcc aaccagatgc tggcagccgt agcgctggta
    12661 ctgggcaccg ttgtgctgat taagatgaag cgcacccaat acatctgggt aactgttgtt
    12721 ccggctgtat ggctgcttat ctgcaccacc tgggcgctgg gcctgaaact gttcagcacc
    12781 aacccgcaga tggaaggctt cttctacatg gcaagccagt acaaagagaa gattgctaac
    12841 ggtactgacc tgacggcaca gcagattgcc aacatgaacc acatcgttgt gaacaactac
    12901 accaacgcgg gcctgagtat tctgttcctg attgtggtgt acagcatcat cttctacggt
    12961 ttcaaaacct ggcttgcggt gcgtaacagc gacaaacgta ctgacaaaga aacaccgtac
    13021 gttccaatcc cggaaggcgg cgtgaagatc tcttcgcacc actaaccggt tgccggatgc
    13081 gttgcgcgta tccggcctac aaagtgtcta tcgtaggccg gataaagcgt ttacgccgca
    13141 tccggcaaat ccctagcccg gaccttccgg gctttacgca tatctggaag gcaaaatgtt
    13201 tggtaactta ggacaggcaa aaaaatatct cggccaggcg gcgaagatgt tgattggcat
    13261 tccagactac gacaactacg tcgagcatat gaagaccaac catcccgaca agccgtacat
    13321 gagctatgaa gaattcttcc gcgaacgcca gaacgcgcgc tacggcggcg atggtaaagg
    13381 cggtatgcgc tgttgttaat ggagagagca tgaacccgat tgcagttacc ctactcaccg
    13441 gttttttagg cgcgggcaaa accaccctgc tgcgccatat tcttaacgaa caacacggct
    13501 acaagattgc cgtgattgaa aacgaattcg gcgaagtctc cgttgatgat caactgattg
    13561 gcgaccgcgc cacgcagatc aaaacgctga ccaacggctg catctgctgt tcgcgctcca
    13621 acgagctgga agacgcacta ctcgacctgc tggataatct cgacaagggc aatattcagt
    13681 tcgaccgtct ggtcattgaa tgcaccggca tggccgatcc cggtccgatt attcagacct
    13741 ttttctccca tgagatttta tgccagcgtt acctgctgga cggcgtgatt gcgctggtgg
    13801 atgcggtaca tgccgatgag cagatgaacc agttcaccat cgcccagtcc caggttggct
    13861 acgccgaccg tattctgctg accaaaaccg acgtcgcagg cgaagcagaa aaactgcgtg
    13921 aacgcctggc gcgcatcaac gcccgtgcac cggtctacac cgtcacccac ggcgacatcg
    13981 acctgggcct gctattcaac accaacggtt ttatgctgga agagaacgtc gtcagcacca
    14041 aaccgcgctt ccactttatc gcggataaac aaaacgatat ttcgtcgatt gtggtggaac
    14101 tggattaccc ggtagatatc agcgaagttt cccgggtgat ggaaaacctg ctgctggaat
    14161 cggcagataa actgctgcgt tacaaaggga tgctgtggat tgacggcgaa cctaaccgcc
    14221 tgctattcca gggcgtccag cgcctctaca gcgccgactg ggaccgcccg tggggcgatg
    14281 aaaaaccgca tagcacgatg gtgtttatcg ggattcagct gccggaggaa gagattcggg
    14341 cggcgtttgc ggggttgagg aagtaatcgt gaaaggttga gtgtaaagca gctcaatgcc
    14401 ccatttagca gtgagggggg aaacagaacg acgtgctccc ctccgctaag ttttattgcg
    14461 cgtgaatacc tggatctggc aaaaaattgc cgcgtttggc gtcagaccat gcttcgctac
    14521 cagaagcagg aatgtcgctg taatcaatgt cttcgtcgga tttattaacc aaagccatga
    14581 gttcagcttc ttgctgcaca tcaagtgtgt agagtgtgcc ccgtttatgt ttaaccatgc
    14641 tcatttatga ccagaacctt tacgatttaa ttataaagat gacttatttc agccatccag
    14701 gcacgttata ttctgagaca ttctcaatgg aatatgcatc tctcctgtca gaacctttct
    14761 tacacttcaa ctcattgacc ccccatttac cctctctatt ggaataaaat acagtatatt
    14821 tatcggtatt aaacccgaaa tatctttcat ccggacgtga aatattcaac tctccacatt
    14881 taacctgatt acgccattca aaatctatgg tgctttttaa aacgtagtac gaaatacctt
    14941 ctacattttt tgaagcaaaa gccactaaaa tagtagatac gataaatgct ccggcaagta
    15001 taaacaacct atatacactt aatttagccc acagagttaa aaaagcaaaa tagataacaa
    15061 gttgcattaa cataaagaaa agcaacataa aagccatccc tttccagaaa tactcaaccc
    15121 atggcgttag tgtgatattg agattactca aacttaaaaa caaactggaa gcacaactac
    15181 ttgcaaacaa atatataatt ggtatgcaag ctataatcat ggaccta
//
LOCUS       DAZGLJ010000110        15133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000110 DAZGLJ010000000
VERSION     DAZGLJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.110"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(155..979)
                     /gene="iclR"
                     /locus_tag="ACHK49_002927"
     CDS             complement(155..979)
                     /gene="iclR"
                     /locus_tag="ACHK49_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO9263544.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            1179..4862
                     /gene="metH"
                     /locus_tag="ACHK49_002928"
     CDS             1179..4862
                     /gene="metH"
                     /locus_tag="ACHK49_002928"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO9263545.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYCESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            5082..6713
                     /locus_tag="ACHK49_002929"
     CDS             5082..6713
                     /locus_tag="ACHK49_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO9263546.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(6804..7493)
                     /gene="pepE"
                     /locus_tag="ACHK49_002930"
     CDS             complement(6804..7493)
                     /gene="pepE"
                     /locus_tag="ACHK49_002930"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO9263547.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(7876..9105)
                     /gene="sorE"
                     /locus_tag="ACHK49_002931"
     CDS             complement(7876..9105)
                     /gene="sorE"
                     /locus_tag="ACHK49_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO9263548.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            complement(9157..9980)
                     /locus_tag="ACHK49_002932"
                     /pseudo
     CDS             complement(9157..9980)
                     /locus_tag="ACHK49_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(9991..10788)
                     /locus_tag="ACHK49_002933"
     CDS             complement(9991..10788)
                     /locus_tag="ACHK49_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO9263549.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQPQATTSTALDQLDD"
     gene            complement(10854..11348)
                     /locus_tag="ACHK49_002934"
     CDS             complement(10854..11348)
                     /locus_tag="ACHK49_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO9263550.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            complement(11348..11755)
                     /locus_tag="ACHK49_002935"
     CDS             complement(11348..11755)
                     /locus_tag="ACHK49_002935"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO9263551.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            complement(11765..12571)
                     /locus_tag="ACHK49_002936"
     CDS             complement(11765..12571)
                     /locus_tag="ACHK49_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9263552.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(12641..13588)
                     /locus_tag="ACHK49_002937"
     CDS             complement(12641..13588)
                     /locus_tag="ACHK49_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO9263553.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELRIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            13936..14808
                     /gene="rluF"
                     /locus_tag="ACHK49_002938"
     CDS             13936..14808
                     /gene="rluF"
                     /locus_tag="ACHK49_002938"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO9263554.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEVKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(14809..15081)
                     /locus_tag="ACHK49_002939"
     CDS             complement(14809..15081)
                     /locus_tag="ACHK49_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO9263555.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
BASE COUNT         3581 a         3976 c         3993 g         3583 t
ORIGIN      
        1 gtagtttgat gtttaaaaat aatgctgagt taataaaagt gtgtgaagtg tatgacatta
       61 agttattttg gcgtcaatgc gattaacaga cacccttatt ctattgccac tcaggtatga
      121 tgggcagaat attgcctctg cccgccagaa aaagtcagcg cattccaccg tacgccagcg
      181 tcacttcctt cgccgcttta atcaccatcg cgccaaactc ggtcacgcgg tcatcggtaa
      241 tacgtgaaat cggtccggaa attgaaattg cggcaaacgg ttcgcggtgc tcatcgaaaa
      301 tacacgctgc aaggcaacgt agccccagtg catgttcctc atcgtcaaat gaataacccc
      361 gtttgcgcgt ttgggcgaga tcttctttta aatgcacagg agacaccagc gttgcgtggg
      421 tataggcatg taaccctttg cggtgcagca gcttcgtcac ctgttcttcg ctcagttggg
      481 ctaaaaaggc tttacccgca ccggaagcgt gcatcggcaa tttaccgccg ataggcgcgg
      541 acattcgcat cagatgcgta cactgtacct ggtcgataat aatcgcttcg tgatcgcttt
      601 gatcaagcac cgccatattg accgtttcgc cagactcttc cattaaattg cgcaggatag
      661 ggtgaacaat cgctaacaaa ttacggctct ggagaaagct gctgccgacc ataaaggcat
      721 gtgcgccgat tgcccaatgt cccagttcgc cgacctgacg cacgaaaccc tgctgttgca
      781 tcgtggttag caggcggtgg gtcgtggaat tgggtaaccc ggcttgttgc gccagctctg
      841 tgagtgccac actgccattg gattcggcaa tccactccag taatttcagg ccacgcgtta
      901 aagactgaac ctgtccagtc gctggtgcgg tggcaacggc gggttttctg ccgcgtttcg
      961 cgggaatggg tgcgaccatg acagcctcct ttttctgtat cgtggaaatc attttcattt
     1021 ttattgttag ctaatgcaat agttgctgaa ctgatccgat gagttaatgt tgaacaaatc
     1081 tcatgttgcg tggtggtcgc ttttaccaca gatgcgttta tgccagtatg gtttgttgaa
     1141 tttttattaa atctgggttg agcgtgtcgg gagcaagtgt gagcagcaaa gtggaacaac
     1201 tgcgtgcgca gttaaatgaa cgtattctgg tgctggacgg cggtatgggc accatgatcc
     1261 agagctatcg actgaacgaa gccgattttc gtggtgaacg ctttgccgac tggccatgcg
     1321 atctcaaagg caacaacgac ctgctggtgc tcagcaaacc ggaagtgatc gccgctatcc
     1381 acaacgccta ctttgaagcg ggcgcggata tcatcgaaac caacaccttc aactccacga
     1441 ccattgcgat ggcggattac cagatggaat ccctgtcggc ggaaatcaac tttgcggcgg
     1501 cgaaactggc gcgcgcctgt gctgacgagt ggaccgcgcg cacgccggag aaaccgcgct
     1561 acgttgccgg tgttctcggc ccaaccaacc gcacggcgtc tatttctccg gacgtcaacg
     1621 atccggcatt ccgcaacatc acttttgacc agctggtggc ggcctattgc gaatccacca
     1681 aagcgctggt ggaaggtggt gcggatctga tcctgattga aaccgttttc gacaccctta
     1741 acgccaaagc ggcggttttt gcggtgaaaa cggagtttga agcgctgggc gttgagctgc
     1801 cgattatgat ctccggcacc atcaccgacg cctccgggcg cacgctctcc gggcagacca
     1861 ccgaagcatt ttacaactca ttgcgccacg ccgaagctct gacctttggc ctgaactgtg
     1921 cgctggggcc cgatgaactg cgccagtacg tgcaggagct gtcacggatt gcggaatgct
     1981 acgtcaccgc gcacccgaac gccgggctac ccaacgcctt tggtgagtac gatctcgacg
     2041 ccgacacgat ggcaaaacag atacgtgaat gggcgcaagc gggttttctc aatatcgtcg
     2101 gcggctgctg tggcaccacg ccacaacata ttgcagcgat gagtcgtgca gtagaaggat
     2161 tagcgccgcg caaactgccg gaaatccccg tagcctgccg tttgtccggc ctggagccgc
     2221 tgaacattgg cgaagatagc ctgtttgtga acgtgggtga acgcaccaac gtcaccggtt
     2281 ccgctaagtt caagcgcctg atcaaagaag agaaatacag cgaggcactg gatgtggccc
     2341 gccagcaggt ggaaaacggt gcgcagatta tcgatatcaa catggatgaa gggatgctcg
     2401 acgccgaagc ggcgatggtg cgttttctca atctgattgc cggtgaaccg gatatcgctc
     2461 gcgtgccgat tatgatcgac tcctcaaaat gggatgtcat tgaaaaaggt ctgaagtgta
     2521 tccagggcaa aggcattgtt aactctatct cgatgaaaga gggcgtcgac gcctttatcc
     2581 atcacgcgaa gctcttgcgt cgttacggtg cggcagtggt ggtaatggcc tttgacgaac
     2641 agggacaggc cgatacccgc gcacggaaaa tcgagatttg ccgtcgggcg tacaaaatcc
     2701 tcaccgaaga ggttggcttc ccgccagaag atatcatctt cgacccgaac atcttcgcgg
     2761 ttgccaccgg cattgaagag cacaacaact acgcgcagga ctttatcggc gcgtgtgaag
     2821 acatcaaacg cgaactgccg cacgcgctaa tttccggcgg cgtatctaac gtttctttct
     2881 cgttccgtgg caacgatccg gtgcgcgaag ccattcacgc agtgttcctc tactacgcta
     2941 ttcgcaatgg catggatatg gggatcgtca atgccgggca gctggcgatt tacgacgacc
     3001 tgcccgctga actgcgcgac gcggtggaag atgtgattct taatcgtcgc gatgatggca
     3061 ctgagcgttt actggagctt gccgagaaat atcgcggcag caaaaccgac gacaccgcta
     3121 acgcccagca ggcggagtgg cgctcgtggg aagtgaataa acgtctggaa tactcgctgg
     3181 tcaaaggcat taccgagttt atcgagcagg ataccgaaga agcccgccag caggctacgc
     3241 gcccgattga agtgattgaa ggcccgttga tggacggcat gaacgtggtc ggcgatctgt
     3301 ttggcgaagg gaaaatgttc ctgccacagg tggtcaaatc ggcgcgcgtc atgaaacagg
     3361 cggtggcgta tctcgaaccg tttattgaag ccagcaaaga gcagggcaaa accaacggca
     3421 agatggtgat cgccaccgtg aagggcgacg tccacgacat cggtaaaaat atcgttggtg
     3481 tggtgctgca atgtaacaac tacgaaattg tcgatctcgg cgttatggtg cctgcggaaa
     3541 aaattctccg taccgctaaa gaagtgaatg ctgatctgat tggactttcg gggcttatca
     3601 cgccgtcgct ggacgagatg gttaacgtgg cgaaagagat ggagcgtcag ggcttcacca
     3661 tcccgctatt gattggaggc gcgacgacct caaaagcgca cacggcggtg aaaattgagc
     3721 agaactacag cggcccgacg gtgtatgtac agaacgcatc gcgcaccgtt ggtgtggtgg
     3781 cggcgctgct ttccgatact cagcgtgatg attttgtcgc tcgtacccgc aaggagtacg
     3841 aaaccgtacg tattcagcac gggcgtaaga aaccgcgcac accaccggtc acgctggaag
     3901 cggcgcgcga taacgatttc gcttttgact ggcaggctta cacgccgccg gtggcgcacc
     3961 gtctcggcgt gcaggaagtt gaagccagca tcgaaactct gcgtaattac atcgactgga
     4021 caccgttctt tatgacctgg tcgctggccg ggaagtatcc gcgcattctg gaagatgaag
     4081 tggtgggcgt tgaggcgcag cggctgttta aagacgccaa cgacatgctg gataaattaa
     4141 gcgccgagaa aacgctgaat ccgcgtggcg tggtgggcct gttcccggca aaccgtgtgg
     4201 gcgatgacat tgaaatctac cgtgacgaaa cgcgcaccca tgtgatcaac gtcagccacc
     4261 atctgcgtca acagaccgaa aaaacgggat tcgctaacta ctgtctcgct gacttcgttg
     4321 cgccgaagct ttctggtaaa gcagattaca tcggcgcatt tgccgtgact ggcgggctgg
     4381 aagaggacgc actggctgat gcctttgaag cgcagcacga tgattacaac aaaatcatgg
     4441 tgaaagcgct tgccgaccgt ttagccgaag cctttgcgga gtatctccat gagcgtgtgc
     4501 gtaaagtcta ctggggctat gcgccgaacg agaacctcag caacgaagaa ctgatccgcg
     4561 aaaactacca gggcatccgt ccggcaccgg gctacccggc ctgcccggaa catacggaaa
     4621 aagccaccat ctgggagctg ctggaagtgg aaaaacatac cggcatgaaa ctcacagaat
     4681 ctttcgccat gtggcccggt gcatcggttt cgggttggta tttcagccac ccggacagca
     4741 agtactacgc ggtggcgcaa attcagcgcg atcaggttga agactatgcc cgccgtaaag
     4801 gtatgagcgt ctccgatgtt gagcgctggc tggcaccgaa tctggggtat gacgcggact
     4861 gattcacaaa tctgtcactt ttccttacaa caaacagggc gctcaatgag tgccctgtct
     4921 ctttattaat atgaaacact tatactggaa acaggctgga ataatcttag ccggaaaaac
     4981 gaatgtcaaa aggcacagta aagatgccag tcagcgtgtt tccggtcctt tggtcattca
     5041 attgcttcag gaacatataa cgataaggag aacctgaact cgtgttaacg ctgcttcacc
     5101 tgctttctgc cgtcgccctg ctggtctggg ggactcatat tgttcgaacc ggcgtaatgc
     5161 gcgtcttcgg cgcgcgtttg cgtactgtcc ttagccggag cgtcgaaaaa aagccgctcg
     5221 ccttttgcgc ggggatcggc gttaccgcac tggtgcagag cagtaatgcc accaccatgc
     5281 tggtgacctc gtttgtcgct caggatctgg tagccctcgc accggctctg gtcattgtgc
     5341 tgggggcaga tgtcgggacg gcgctaatgg cgcgtattct caccttcgac ttatcctggc
     5401 tgtcaccgtt acttattttt atcggcgtga tttttttcct cggacgcaaa cagtcacgcg
     5461 ccgggcaact gggccgcgtc ggtattggtc ttgggctgat tttactggcg ctggagttga
     5521 ttgtgcaggc cgtaacgccg atcacccagg caaacggcgt tcaggtgatc tttgcctcgc
     5581 tgaccggcga tattctgctg gatgcgctga ttggcgcgat gttcgccatt atcagctact
     5641 ccagccttgc tgctgtactg ctgactgcga ctctgaccgc cgcaggcatt atctccttcc
     5701 ccgtggcgct ctgtctggtg attggtgcta acctcggttc cggcctgctg gcgatgctca
     5761 acaacagtgc cgccaatgcc gcagcccgcc gtgtcgcgct gggtagtctg ctgtttaagc
     5821 tggtgggtag cctgattatc ctgccgtttg tccatttgct ggcagagaca atggggaagc
     5881 tgtcattgcc aaaagcggaa ctggtgatct atttccacgt cttctacaac cttgtacgtt
     5941 gcctggtcat gctgccattt gttgacccga tggcacggtt ttgcaaaacg attattcgcg
     6001 atgaaccgga actggatacc cagctacggc ctaaacatct ggatgtcagc gcgctggata
     6061 cgcccacgct tgctctggcg aacgccgcgc gcgaaaccct gcgcattggc gacgccatgg
     6121 aacagatgat ggaagggctg aataaagtga tgcacggcga gccacggcag gagaaagagc
     6181 tgcgtaagct ggcagatgat atcaacgttc tctataccgc cattaagctg tatctggcgc
     6241 ggatgccaaa agaggaactg gcagaagaag agtcgcgtcg ctgggcggag attattgaaa
     6301 tgtcgctcaa ccttgaacag gcttccgata tcgtcgagcg catgggcagc gaaattgccg
     6361 ataaatcgct ggcagcacgg cgggcatttt cgcttgatgg gttgaaggaa ctggatgcgc
     6421 tctatgagca attgctcagt aatttaaagc tggcaatgtc ggtgttcttc tctggcgatg
     6481 tcaccagcgc tcgtcgtttg cgtcgcagca aacatcgttt tcgcattctt aatcgccgct
     6541 attcccacgc ccacgtcgat cgcctgcatc agcaaaacgt gcagagcatt gaaaccagtt
     6601 cgctacattt aggcttactg ggagatatgc agcgcctgaa ctcgctgttt tgttcggtgg
     6661 cttacagtgt gctggaacag ccggatgaag atgaaggacg ggacgagtat taatattgtg
     6721 aatcctgtag gcctgataag acgcgtcaag cgtcgcatca ggcactatga acaagatgcc
     6781 ggatcatgca tccggcaatt tacttaaaaa cggtgaccag cttctagcgg caccgcttct
     6841 tcaccagcct taaacacata agtggtgttc gggccaccca gcgtagcgtg acctttactc
     6901 actgtgatcc agttaccttc cggtagacca ataatcgtca gttctggcgc gacgaccagc
     6961 agttcgcgaa tacgctgctc acgggtttca cctttatggc cttccggcag cgcgttggtg
     7021 aagtgcgggt tgatttgcag cgggaacaga tttagcgcat cgaaaccttg cggatcgaca
     7081 atcggcatat cgttggtggt acgaatggtt gggcaagcaa ggttagcgcc tgcgctccag
     7141 ccaatataca gcgcaccacg tttaaccacg tcagtaattg gtgccagcag cccgcgctcg
     7201 cggcactgtt tcagcaactg gaaagtattc ccgcccccga caatcacgat ctcagcattt
     7261 tcaatcgcgg caacgggatc gacaacgcta tgaataccgg tgacagatac acccagcgga
     7321 gcgagaaccg cagccgtttt cgctgtgtaa tcatcccagg tctgcgttac gccagcgaaa
     7381 ggaataaata ccgctgaacg gcgaccctgc aactgttcag caatcagcgg cagcgcatgt
     7441 tccagccagg ctttacccgg cagcgtcgag ttactcaata aaagcagttc cattacttct
     7501 ccagttagta agaaatcacg ggaggcatgc taccactgcg aatttaacca cttactgaca
     7561 tggctcacga tgtcacgtat tgcaccgggc gctgagactg atgacaaaca cagcattgcc
     7621 tgatgcgcta cgcttaccag gcctaccaga taactgcaat atattgaatt ttcgagatta
     7681 tgtaggccga ataaggcttt attccgcatc cggcataaac aatgtgtact ttgccaccaa
     7741 tttgagcgcc ccttaaggcg ctgatgtttt tttatccttc tcgtgcacat tgggccgctt
     7801 cagcaatcag ccacacatca ggtcgcgaag ttgccgcggc gaccagccag cgacatgcag
     7861 agttaagtag cgcgattaat catgggaaat tgcctctgca tgagtgagca gatattgctc
     7921 cgcctcaccg gaccagatcc cctgatgacg cgccagaatc gccgccagtg cttgatcctg
     7981 aatctgcgtg agtgacgtca gcggcaggta tttcccggta tacaccagct ttttgccgcc
     8041 gccgacggca ggcaattcaa gcgtggtttc gcccgcggca ttcagcccaa gaatatgtgt
     8101 taccactttt gcggcctgca cttttttctc ttcaatcagt ttgaccgccg cgcgcatgtc
     8161 gtcggtattg ccgcctgacg tgcccacgta gtgggtaaat gcataatgca catcgtagaa
     8221 attaattggc gcgctgaaat gtttatcctg cggtccggcg aagaaattca ggcaaccatc
     8281 tgtcgccagc aaggaagagg cgagagtcac cagtccttca ttaggcacaa agacgaaaat
     8341 atcatcgaag ccgtgaccgc cactcagcgc cattagcgta tcaaatgctg catcggcggc
     8401 attgagataa tgaatcagtg tttgcggttc tgatggatag tgcttgcgcg cataactcaa
     8461 tttatcgttg tcggtatcgg taatgacgag cagcgacggg ttaacgggtc catgtagcgc
     8521 ataatcaatc gccaacagtc ccattggtcc ggtgccgccg aggatcagca tgcgcccttg
     8581 cgggcgaatc cccatcgtgt ggttataact accttcctga agatgatagt tggcgttgaa
     8641 cgcgccaatc acgcaggaaa gcggttcaac cagtgagcct tcaaaatagg tttcgccgtc
     8701 gtatgccagc aggcaatctt gttccatgac ctcgttggga ataaccacat gcgtggcttc
     8761 gccgcccacc cacgggaagg agtagccggg gcagtccggg cggtcgggga gttgcaggtt
     8821 ggcctgaatc acataacgct gacccggctg gaatttgtgc tgccactttt tacccacggc
     8881 cagaatatcg ccgcaaaact cgtggccgat gatgatgggg ttggtcgcca catcgtcggg
     8941 tactttttta tggttttcac ccagattggc ctctttccag gaagagaggc acaggctgtc
     9001 agtgaccacc gtcgcgagga tttcatcctc ctgcatttca ggaaggtcaa aggtttccag
     9061 gcgtaaatca cgtttaccat aaagacgcag agctgttgtt ttcataaacc acctcatgtt
     9121 gagcgatggc cccggcagtc gtgccggggc gaagaatcag gacaggaagc ccagcgcatt
     9181 accgataatc cctaagccaa acagggcgaa aatcagccat accgggttaa tttttttgtt
     9241 gagcagacga accataagca gcgtcagacc gagcgccagc aaaccagggc aaagttggtc
     9301 gagaatgttc tgcacggtca tggtgacggt ggagccatcg gcggcatgcg tttgtgaaac
     9361 caccaacggt acgttgattg acgtccattt ggtgaccagc acgcccatca caaacagtcc
     9421 gagaatcgac gcgccttcgg tgagtttttg cagcacattc ccgcccatat cgctgacgat
     9481 attcacccct ttgcgaaagc cgagctgtag gccataccac ttcatcgcca gacgcaccgc
     9541 attgaaaata aagaaaaaaa aagcgggccg aggatgttgc cagaaagtgc cagagatgcg
     9601 ccgagcgcag cggtaatcgg gcgcagcgtt ccccaaacca gtggatcgcc aacccctgcc
     9661 aatggtccca tcagaccgac tttgatgccg ttaatggtac cgtcatcaat ttccgcgccg
     9721 ttggcccgcg cctcttccat ggcggcagtg acgccgatga ccgggccaca tacggctggc
     9781 gtggtattga agaacaccag gtgtcgcctg agcgccgcaa cctgatcctc ttttaatggg
     9841 taaagtcgct tgatggcggg gatcatgtcg tagcaaaagc ccagcccgtg aatacgttca
     9901 aagttaaagg acgcctgttg caggttggag cgcagaaaca tgctcaccag atcgctgcgt
     9961 gtaatttttc tctgttccat gatggctccg ttaatcgtca agttggtcaa gggcggtgga
    10021 ggtggtggcc tggggttgag gttcagcttt acgccactgt ggatttagct ggatgtagac
    10081 cagggccatg atcacgccga cgccaccgaa cgccagcaga ctgagatcga ggtagccacc
    10141 tgcgaggaaa ccgaggaaaa agaaaggcat caaatatttc acgcccatca tacgtagcac
    10201 catggcgtaa ccgaccacca cgataaaacc gccagcaatt tgcagaccgc gcgtcacgaa
    10261 ttccggtatc gcgctcagca tattgctcac catatcggcg ctgacgaaca gtgagacaat
    10321 cagtgccgga atagcaacgc gcagcgcctg cacgccaagc gcggagacat gcagaatatc
    10381 gagagtgcga aaccgcgctt cttccgctgc tttatccgcc gcgtgctgga acactacggt
    10441 gatggtacgg gcaaacacag tcagcacctg gcctgccgca gccacaggca acgcgatggc
    10501 gattccggtg gcgatgcttt gctgaccaac gataaccaga atggcggaga ttatgctggc
    10561 gagtgcagaa tccggagatt gcgccgcgcc gacgttcatc cagccgagag ctatcaattc
    10621 cagcgtacca ccgagtatta ttccggtttt taaatcaccg agaattaaac caatcaccgt
    10681 acaggcaatt aacgggcgat gagtctgaaa ttcatccagc acgctgccca ttccggcaat
    10741 acaggaaaaa agaaatatag caatgatttg tagggtactt atttccatga tttttcacct
    10801 tttaagtgtc gtcatccctg cctgttaaag catatgtaca caggcgctat tatttaattt
    10861 gcgattaact gttcgtttat tttgtcgata atattgattg agggatctga cgctacgacg
    10921 cgtaaatcca gtatgacgcc aagattattt aactcatgaa atgcgttaat atcatcatca
    10981 tctaatgaaa ccgctttggt taactgtttt ttaccgggcc gccaggccat gccgccaata
    11041 ttcagggtgt ctatttttac cccctggcga accatcgcta aggcatcctg tgggcgagtg
    11101 aaaagataaa aaacggtttc gtcctgatat tgcgggttgt gataaacagc gacggctttt
    11161 tcaatattga ccacgttaac cttcattccc ggtggcgcag cctgacgcaa taatgtccgg
    11221 cgaacttcat cgttataaac ttcgtcattg cagataataa tgcgctgggc gttagccact
    11281 ttcgaccaga cggtagtgac ctgaccgtgg atcaggcggt catcaatgcg ggcgagcgta
    11341 atgttcatca gaaatcctcc tctgccgttg ccagttgttt ccagacaacg caggtttgct
    11401 ttgcgatttg cgtcaggtgt tcacacagtt catcaacatt catgctgtcc tggttatcaa
    11461 ccagttcgag cgccagcggc agagaaaggc cgctgattac ccgcagacga ggattgcgca
    11521 tcgcaagcat cgctgccgca ttccacgggc tgccgcactg taaatccacg gcaatcagcc
    11581 actcatcttg attgtgaatg cttactaact tttccagctt tgctacgatg tctccggcgt
    11641 tctcgccggg cacaaattca acggcttcaa cattggcgtt gccgtaaacc atctgaaccg
    11701 attccagcat ggcgcaagct aatttgccgt gggcacaaaa aatggcgttg accataaagc
    11761 ctccttagcc gcgcgttttg ccgcccgcaa tgttagtggt tactccggtg atgtagctgg
    11821 cgcgttcaga caacagataa caaacaaaat cagcgacttc tgctaatctt ccggcgcgcc
    11881 caataggaat ggcgtttttg gtatagcctt cacgcagctg ctcgacggtg atattgcgcg
    11941 tccacgccag cgcctcttca tattccggcg tgcgtagccc ggttttttcc agaatcccgg
    12001 gcgcgatacc gaccacacgg ataccgtgtt tacccagctc tttcgaccag gagcgcgtga
    12061 agctattaag cgcggctttg gtagcggcgt aacagctttg gccttctgag ccttccagtc
    12121 cactttctga ggaaacattc acaatcacgc catcatgttg tttgaccatc tgtcgcgcca
    12181 ccgcctgcga catcagaaaa acgcctttct ggttgatatt gaccattttt tcgaatgcag
    12241 cttcgttgag ttcatactgc ccggcaggcg ctttctcatc gaccagcaga cgcgggaaat
    12301 tgaccccggc gttattgacc agaccgtcga tgcgaccaaa acgctggata atttccgcta
    12361 ccgtatgatt tacctctttg gcgctggaaa tatcggtcgg ccaaaactga tagcctttat
    12421 gaccttcata ttgaccatcg ccaccgtgaa tatctaccat ctgtacgttt gcgccttgtg
    12481 ctaataattc ctctacaatc gccagaccaa taccggatgc gccgccggtg acgataatga
    12541 ttttatcctg caaatttaac cacgtttgca taacagactc ctgattttag ataataggga
    12601 cccgccgtaa tttttattac ggtaaacatc gggaagattt ttatttcagt aatagttcag
    12661 ctgtgctgct attcgttacc agacaattaa tatattttcc acgcagtgca ccaataattc
    12721 cgctgtattt ttcttcactc atggcaatgc caatggaata tcgcgcctgc tttaatttat
    12781 tcatttcgat agagagcgtt ttttcgctca tattcgtttc aaccattgcg ccgtgaatat
    12841 caaaaaagcg cgagcaaata tcgccagcaa cctggcgggc attcaggtcg tcactctctt
    12901 caccaccata aaacgcatgc cagttagcgc cgtcgcgaat ggccggtgag ccaattccca
    12961 ccagggcgac atccagatta tcccagtagg cagagatggt tttaaagtgc tgagactgca
    13021 tgatcccatt acgaattaat gggttatcca gcagagccgg aaaatccgcg agatgcgatt
    13081 cccctttcag cttcgctgcc gcgctgtagg ttaatgtgtt tacatgatag cggctttcga
    13141 gtttaccgga cggtccgcca ataattggta cgcagattaa ctgccgcgat tgccccgcct
    13201 gcggcaagtt ttcaaccagt gcgctcaccg cgcgacccca ggaaaaaccg acaatatcgc
    13261 caggctccag caagcgatcc agcagttgcg cgccgtgtaa ccccatcatc gccagttgag
    13321 tctcttcatc ctcatcattt cccgacacca ccacaacgtc tttcaggcca aacttttgct
    13381 tcagttgctg ctccagccag agattttcgt tgtagtcata gttgatggcg atggtgacaa
    13441 ttccctgatc gcggcctcgt ttaagcaagc ggctgatggt ggtgcggtaa atacgcagtt
    13501 cgcgcgcgat ttgcgcctgc gtcatatcct gttcgtaata gagttgggca atcttcacaa
    13561 tcaaacggat atcgtcactg ttttccattg ccgcctcctg gttctgcaca tttgtgcaaa
    13621 tacgctttct caccgcttga tctgatataa ccccatcatg agggcctttc aaagcgattt
    13681 ttcactcttt gcatggggag aaatagcgct ttctcttgca catttgtttc tctgcgtagt
    13741 agtcatcagc acaggtgtgc agcgattcgt aatatgttga ttatccttat tttgccaggc
    13801 aaatttgcat cgtacttccg gagacacttt tgtgataaaa ggcgtggcag atcacattta
    13861 atttcgacgt tgccgcataa ccctgttttt ccccgctaag aggtacggta tattccgcgt
    13921 tttacaggag aatttatgct gcccgactca tcagtccgtt taaataaata catcagcgaa
    13981 agcggaattt gctcacgccg cgaagcggat cgctatatcg agcaaggcaa tgtgttcctt
    14041 aatggcaagc gagccaccat tggcgatcag gtgaaacccg gcgacattgt gaaagtaaac
    14101 ggtcagttga ttgagcctcg ggaagccgaa gatttggtac ttatcgccct gaacaagccc
    14161 gtcggtattg tcagcaccac cgaagatggc gagcgcgata acattgtcga tttcgttaac
    14221 cacagcaaac gcgtgttccc gattggccgc ctggataaag actcccaggg gctgattttc
    14281 ctcaccaatc acggcgatct ggtgaataag atcctccgtg cgggtaacga tcatgagaaa
    14341 gagtatctgg tgacggtcga taaaccgatt accgatgaat ttattcgtgg tatgggggcg
    14401 ggggtgccga tcctcgggac ggtgacgaaa aagtgcaaag tcaaaaaaga agcgccgttt
    14461 gtcttccgca ttaccctggt gcaggggctg aaccgtcaga tccggcgcat gtgcgagcat
    14521 ttcggctatg aagtgaaaaa gctggaacgt acgcgcatta tgaacgtcag tttaagcggc
    14581 attccgctgg gtgagtggcg cgatttaact gacgatgagt taatcgacct gtttaaactc
    14641 attgaaaatt cctcttccga ggtaaaacct aaagcgaagg ctaaaccgaa aacagcgggc
    14701 atcaaacgtc cagtcgttaa gatggaaaaa acggcggaaa aaggcggtcg cccggcgtcc
    14761 aacggtaagc gttttacctc gccggggcgt aaaaagaagg gacgctaatc agcgcctgcc
    14821 gcgcgtcgac gtatttgctg accagctaaa tgacgcttca ggatccggtt tataagcctg
    14881 ctttttcttc aactggcggg cttttttcgc ttcagcctca cgctcgacca ttaatttatc
    14941 gatgtactct ttcttaatgc tgttggtttc ggagttggtc agtacgcgac catgcgcaat
    15001 ccgcgcccga tccagcagtg ttttaagttc acgctgctcg cgttcagtca tctctttttg
    15061 ggtaatacgg gggagtgcca tgatggtgcc ctcagtgagc cagagagttc agtgtacggg
    15121 aattcgtttg cga
//
LOCUS       DAZGLJ010000111        14995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000111 DAZGLJ010000000
VERSION     DAZGLJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.111"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(183..3629)
                     /gene="mfd"
                     /locus_tag="ACHK49_002940"
     CDS             complement(183..3629)
                     /gene="mfd"
                     /locus_tag="ACHK49_002940"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO9263556.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            complement(3757..4830)
                     /gene="ycfT"
                     /locus_tag="ACHK49_002941"
     CDS             complement(3757..4830)
                     /gene="ycfT"
                     /locus_tag="ACHK49_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO9263557.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLLYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            5092..6291
                     /gene="lolC"
                     /locus_tag="ACHK49_002942"
     CDS             5092..6291
                     /gene="lolC"
                     /locus_tag="ACHK49_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO9263558.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            6284..6985
                     /gene="lolD"
                     /locus_tag="ACHK49_002943"
     CDS             6284..6985
                     /gene="lolD"
                     /locus_tag="ACHK49_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO9263559.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            6985..8229
                     /gene="lolE"
                     /locus_tag="ACHK49_002944"
     CDS             6985..8229
                     /gene="lolE"
                     /locus_tag="ACHK49_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO9263560.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            8258..9169
                     /gene="nagK"
                     /locus_tag="ACHK49_002945"
     CDS             8258..9169
                     /gene="nagK"
                     /locus_tag="ACHK49_002945"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO9263561.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            9185..10006
                     /gene="cobB"
                     /locus_tag="ACHK49_002946"
     CDS             9185..10006
                     /gene="cobB"
                     /locus_tag="ACHK49_002946"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO9263562.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(10145..10933)
                     /gene="ycfZ"
                     /locus_tag="ACHK49_002947"
     CDS             complement(10145..10933)
                     /gene="ycfZ"
                     /locus_tag="ACHK49_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO9263563.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(10930..11391)
                     /locus_tag="ACHK49_002948"
     CDS             complement(10930..11391)
                     /locus_tag="ACHK49_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO9263564.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSCP"
     gene            complement(11449..12495)
                     /gene="potD"
                     /locus_tag="ACHK49_002949"
     CDS             complement(11449..12495)
                     /gene="potD"
                     /locus_tag="ACHK49_002949"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO9263565.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(12492..13286)
                     /gene="potC"
                     /locus_tag="ACHK49_002950"
     CDS             complement(12492..13286)
                     /gene="potC"
                     /locus_tag="ACHK49_002950"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO9263566.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(13453..14571)
                     /locus_tag="ACHK49_002951"
     CDS             complement(13453..14571)
                     /locus_tag="ACHK49_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO9263567.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            complement(14540..14809)
                     /locus_tag="ACHK49_002952"
     CDS             complement(14540..14809)
                     /locus_tag="ACHK49_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO9263568.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            complement(14871..>14995)
                     /locus_tag="ACHK49_002953"
     CDS             complement(14871..>14995)
                     /locus_tag="ACHK49_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO9263569.1"
                     /translation="DARTAIPFEGERHNALDDARHQAKYVSAIWQKLIPNQADF"
BASE COUNT         3580 a         3759 c         3861 g         3795 t
ORIGIN      
        1 aaacgttagc cagcgagaaa aacgcgtttt gatcatcacc aaaaatccat aataaatata
       61 aggttattgt aataaacaga taagcctgaa ttatggatgg tgacagtgtc ggatagtgca
      121 gggaagtgca aagaatttgt aaatgttgca gatgggggcg cagaaacgcc cccgatttac
      181 cattaagcga tcgcgttctc ttccagttca cgcataaact ggcgtaccca ttcgatacgc
      241 gttttccgct cactcaaatc ctgaataaat ttcaggcgcg tcggaccatc aaggcggtaa
      301 tgctgcggct gtttttgcag caaaccaatc aaccaggccg gattaacgtg attcttctcg
      361 gcaaattcga tcaccccgcc tttctcatta ccttccagct tcctgatccc cagtttctgc
      421 gcttgctggc gcagacgggc aacatccagc agggtacgcg ccggatccgg cagcaggccg
      481 aagcgatcga taagctcgac tttgatctct tccagttcgt tttccgtttt ggcgctggca
      541 atacgtttat agaacgacaa acgcgtattc acgtcaggaa tgaaatcatc tggcaatagc
      601 gacggcatcc gcagctcgac ttctgtttgc tggctggtga gatcttccag cgacggctcg
      661 cgtccggctt tcagtgcatc gacggcgttt tccagcaact ccatatacag cgagaaaccg
      721 atggtttcca ttgagccgct ttgttcttcg ccaagcagtt cacccgcgcc gcgaatctcc
      781 agatcgtgcg ttgccagcgc aaaacctgcc ccgagatctt ccagcgaggc aatcgcttca
      841 agacgttttt gtgcatcggt agtcatcgct tttggatgcg gcgtcagcaa ccatgcatat
      901 gcctgatgat gtgaacgtcc gacgcgaccg cgtaactggt gcagctgcgc cagaccgaag
      961 tgatccgcgc gttcaatgat aatggtgttg gctgtcggga tgtcgatccc ggtttcgata
     1021 atggttgtac aaaccagcac gttgaaacgt tgatgatgga aatcattcat cacccgttcc
     1081 agttcgcgct cgcgcatctg cccgtgaccg atggcgatcc gcgcttctgg caccagttct
     1141 gccagccgtt cggcggcttt ctgaatgttt tccacatcat tgtagagata ataaacctgt
     1201 cctccgcgca aaatttcacg caggatcgcc tcccggacca ccaggctgtc atactcacgg
     1261 acaaaggttt taactgccag acgacgggcg ggcggcgtgg cgataatcga caggtcacgc
     1321 attccgctca ttgccatatt cagcgtacgt gggatcggcg ttgcagtaag cgtcagaata
     1381 tccacgttcg cgcgcatcgc tttaatacgc tctttatgac gcaccccgaa gcggtgttct
     1441 tcatcgacaa tcagcaggcc taaatcttta aacttgacgt cactttgcag cagtttgtgc
     1501 gtaccgatca gaatatcgat tttcccttcc gccacttccg caaggatttg cgtctgctct
     1561 ttggcgctgc ggaaacggga gatcatttcg atacgtaccg gccagttggc gaaacggtcg
     1621 cggaagttgt cgtaatgctg ctgcgcgaga agggtggtag gcaccaacac cgccacctgc
     1681 ttgtggttat ctactgccag gaaagcggcg cgcatcgcca cttctgtttt accaaagcca
     1741 acatcgccgc acaccagacg atccattgcc agcggctgac acatgtcgct aagtaccgca
     1801 ttaatggcct gcgcctgatc cggcgtggtt tcaaacggga agctgtcgca gaacaactga
     1861 tactgctcac gatcgtgttt aaacgcgaag ccctctttgg cggcgcgttg cgcgtagata
     1921 tccagcaatt ccgccgccac atcacgcact ttttccgccg ctttctgccg cgcgcgtgac
     1981 cacgcatcgc cgccaagttt atgcagcggg gcgttttctt ccgcgccacc tgcgtaacgg
     2041 ctaatcagat gcagtgacga caccggaaca tacagtttgg cgtcgttggc ataggtgagc
     2101 atcaaatact cgccagtaat gccgcccgct tcgagcgtgg tcattccggc atagcgcccg
     2161 acaccgtgct ccagatggac caccggctga ccaatatgca gctccgcaag gttacggatc
     2221 agtgtatcgg ggttgatggt gcggcgagaa tcctgacgac ggcgggcaac acgttcaccg
     2281 agcagatcgc tttcgcagat cagcgccaga ttacgcacct tatcgacaaa accatgttcg
     2341 gcagcgccaa tcatcagata acgcccacgg tcgctggctt catcaagacg cataatgcgt
     2401 tgcggagcaa ttttaattcg tgcgagcagt tcacccagcg cttcacggcg accttcactt
     2461 tctaccgaga acaccaccgg accgtcgaaa gtctcgagga acttacgcag cgcatccagc
     2521 ggcgcttttt gttgtgcctg aacggccagg tctggcagtt tctggaaacc taaattggca
     2581 ttcgcggctt ttgtcggtaa atgttcagtt tttagctgca cccggggcca gtttttcagc
     2641 tctgagaaga gctcgtccac ccgcagccag agcgattgtg gtggcaacag cgggcgcatc
     2701 ggatcgacgc cgcgattctc aaaacgcgcc agcgtgtcag cctggaaacg ttcggcactg
     2761 gtttccagat cgccagtatt cacgagcaag gtattggcag ggaaataact gaacagcggc
     2821 ggcagtggtt cgctgaagaa caatggctgc cagtactcga tcccggcagg taatgtgcct
     2881 ttactcactt gctggtaaat atgttccgga tcgcgcttca cttcgaaggt atcgcgccac
     2941 tggctgcgga acagttcaat tgccgcttta tcggtcggaa attcgtgcgc gggcagcaga
     3001 ttgatcgctt ctacttcctc cagcgtgcgc tggctgtcga cgtcaaacac ccgcaggctg
     3061 tcgatttcat catcaaagaa atcaagacga taaggcagct cactccccat cgggaagaga
     3121 tccagcaacg cgccgcgcgt ggcgtattcg ccgtgctcca tcacctggtc aacatggcga
     3181 taaccggcgc tgtccagttg ggttcgtaat gcatctcgtg acaggcgctg accttttttc
     3241 atcaccagcg catgaccgtg gagaaaactg tgtgggcaaa cgcgctgcat aagcgtattc
     3301 accggaacaa tcagtacgcc acgctgcatc gtcggtagct ggtaaagggt ggaaaggcgc
     3361 gaggagataa tgtcctgatg aggcgaaaaa ctgtcgtagg gaagagtttc ccagtccgcc
     3421 agattcatca ccatctgatc ggtgaactgg ctgatttcat catgcaaacg cagagcattt
     3481 tgcatatctg gtgcaatgag taccaccgga ccggcgtgac gttcggcaat ttccgctacc
     3541 agcgttgcac aggctgcgcc ggttaactcg cccagcagac gctgctcacc cgctttgacg
     3601 ggcagcgtat aacgatattg ttcaggcata acggttgtca gattctcgtt aggatatgcc
     3661 tcaacatatg ggggcatatc tctgataagc aatgctgttc attatccgta atcgacatgg
     3721 tttagcaaat tgaatcgccc cggctggggc gatggtttaa cgagagtaac tgacggcagc
     3781 gggcagtgaa ggcggggaga acaacagatc gctgaatgca cgctgtgaaa gttttcttac
     3841 cagcaagccc gcaacagtac agatgaacaa actgacaaag ggataaacca gcaggagtgt
     3901 cagttcgact tgcggcgacc agcgtgctgc gttcatttgc gcaagcagag ttaagctgaa
     3961 tatttcaacc agaatgcgat gggtggtgta gatagcaatg gtgttggagc caatcacatt
     4021 cagcaggctg gtggagcgca taccgaatcg ttgctcgtat tgataaaaca gcttcatgat
     4081 caccacaatc gataccagcg acaacagcag cgagatagta aacaaccagg caccgaccgc
     4141 cagaacggta agcaaagaag ccatcagcag atggcggcgc aacggcacct ctttaacaca
     4201 ggtcattatt gttgcgccaa accatgcgcc aaggctgtaa taaagcaaat tgcggatcac
     4261 actgttcatt ccccaccacg gcgtgggaac gaaattaacc gccacactca gcagtacaaa
     4321 caaggcgaat agtggcagcg ccagacggct aaaaatttta cataccacga aatagacaat
     4381 taacgcatac agataccaca agctggtgct ggcggtgatc atcccgtgca ggaactcacc
     4441 ggtggaatcg gcataagcgg cattggaggc attgcttaaa tcgcgctcag gtgccagcca
     4501 ctggttcagc gcacttaacg ccagccactg caccacgccc caaagcgcca gcacccagaa
     4561 gatgttccag atccgtttat cgagacaatt tccccacggt acgctgtcga tatagcggcg
     4621 aatcaaatag ccggatataa agaaaaaaac cggcatacga aagggggcaa ggtaaagatt
     4681 gaaatagatc cagcatttgc tcaggacttc cgataacgga tgctgaaaag tggtcagatg
     4741 cggataaaag gtaatgaccg agtgataaat caccaccaga caaatacata accctttgat
     4801 ctggttaatc catagctctt tttgtttcat caggacaaca cgctcttttt taatttacat
     4861 gcggttgatc ctgcgggtta tgacccgagg tgtaatgggg atctgtctgt attggatgat
     4921 ttttcagatt aagatcaggc ggcaagattg atgataaaac atggcaattt agccgattga
     4981 tttacggggg cttttcagat tagccctgac gatcacttac agttcagacg tttacccatc
     5041 ttgctttcgc ttatatactc gtgtctttgc tacagcaacc agacggattt catgtaccaa
     5101 cctgtcgctc tatttattgg cctgcgttac atgcgtgggc gtgcagcgga tcgcttcggt
     5161 cgtttcgtct cctggctttc taccatcggc attaccctcg gggtgatggc gctggtcaca
     5221 gtattgtcag tgatgaacgg ctttgagcgc gagctgcaaa acaacatcct tggcctgatg
     5281 ccacaggcaa ttctctcttc tgagcatggc tctcttaacc cgcagcaact cccggaaacg
     5341 gcagtcaaac tggacggcgt taatcgcgtc gcacctatta ctaccggtga tgtggtactg
     5401 caaagcgcgc gcagcgtggc ggtcggggtg atgctcggta tcgacccggc gcaaaaagat
     5461 ccacttacac cgtatctggt caatgtgaaa caaactgacc tcgagccggg gaaatataat
     5521 gtcatcctcg gcgaacaact tgcctcacag ctaggcgtta atcgcggtga tcaaatccgc
     5581 gtgatggtac catctgccag ccagttcacg ccgatggggc gtattccaag ccagcgcctg
     5641 ttcaatgtga ttggcacttt cgccgccaac agtgaagtcg atggctatga aatgctggtg
     5701 aatattgagg atgcctcgcg tctgatgcgt tatccggcag gcaatattac cggctggcgt
     5761 ttgtggctgg atgagccgct gaaagttgac tctttaagtc agcaaaaact gcctgaaggc
     5821 agcaaatggc aggactggcg tgaccgtaaa ggcgagctgt tccaggccgt acgcatggaa
     5881 aaaaatatga tgggcttgct gctgagcctg attgtcgccg ttgcggcgtt taacattatt
     5941 acctcactag ggctgatggt aatggagaag cagggcgaag tcgcgatcct gcaaacgcaa
     6001 ggcttaactc cgcgacaaat catgatggtc tttatggtgc aaggggccag cgccgggatt
     6061 atcggtgcga tcctcggtgc ggcgcttggc gcactgcttg ccagccagtt aaataatctg
     6121 atgccgataa tcggcgtcct gcttgatggc gcggcgctgc cggtcgctat cgaaccttta
     6181 caggtcattg ttattgcgct ggtggcgatg gctatcgcgc tgctgtctac gctttaccct
     6241 tcatggcgcg ctgccgccac tcaacccgct gaggctttac gttatgaata agatcctgtt
     6301 gcaatgcgac aacctgtgca aacgctatca ggaaggcagt gtgcaaaccg atgtgctgca
     6361 caatgtcagt ttcagcgtcg gcgaaggtga aatgatggcg atcgtcggta gctctggttc
     6421 cggtaaaagc accttgctgc acttgttggg cgggctggat acgccaacct ccggcgatgt
     6481 gatctttaac ggtcaaccga tgagcaaact gtcttcggcg gcgaaagcag agctgcgcaa
     6541 ccagaagctg ggctttattt atcagtttca ccacctgctg ccggatttta ccgccctgga
     6601 aaacgtggct atgccgctgc tgattggcaa gaaaaagccc gctgaaatca acagccgtgc
     6661 acttgagatg ttaaaagcgg tggggctgga tcatcgtgcg aatcaccgcc catctgaact
     6721 ttctggcggc gaacgccagc gtgtggcgat tgcccgtgcg ctggtgaata acccgcgcct
     6781 ggtactggcg gatgaaccta ccggtaacct cgatgcgcgt aacgccgaca gcatcttcca
     6841 gttgcttggg gaattgaatc gcttgcaggg caccgccttc ctggtggtta ctcacgacct
     6901 gcaactggcg aaacgtatga gccgccagtt agaaatgcgt gatggtcgtc tgacggcgga
     6961 actgagcctg atgggggcgg agtaatggcg atgcctttat cgttattaat tggcctgcgt
     7021 tttagtcgcg gacggcggcg cggcggcatg gtgtcgctga tctccgttat ttctaccatt
     7081 ggcattgccc tcggcgtggc ggtattgatc gtcggcttaa gcgcgatgaa cggctttgaa
     7141 cgcgaactga acaaccgtat tctggcggtg gtgccgcatg gtgaaatcga ggcggtggat
     7201 cagccgtgga ctaactggca ggaagcactg gatcacgtgc aaaaagtgcc gggtattgcc
     7261 gctgccgcgc cgtatatcaa tttcaccggg ctggtggaaa gtggcgcgaa tcttcgcgca
     7321 atccaggtga agggcgttaa cccgcaacag gaacagcgtc tgagcgcatt accctcgttt
     7381 gtccagggcg atgcgtggcg caattttaaa gcgggcgaac agcaaattat catcggcaaa
     7441 ggcgtggcgg atgcgctgaa agtgaagcag ggggattggg tgtcgattat gatccccaac
     7501 tcgaatcccg agcataaact gatgcagcca aaacgtgtgc gtttgcacgt tgccggtatt
     7561 ttgcagttga gtggtcaact cgatcacagt tttgccatga tcccgctggc ggatgcccaa
     7621 caatatcttg atatgggttc cagcgtgtca ggtattgccc ttaaaatgac ggatgttttc
     7681 aacgccaata agctggtacg cgatgcgggt gaagtgacca acagctatgt ttatattaaa
     7741 agttggattg gtacttacgg ctatatgtat cgcgatatcc agatgatccg cgccattatg
     7801 tatctggcga tggtactggt gattggcgtg gcctgtttca acatcgtctc caccttagtg
     7861 atggcggtga aagacaagag tggcgatatc gcagtattaa gaacgctggg ggcgaaagat
     7921 ggtttaattc gcgccatctt tgtctggtat ggattgctgg cagggctgtt cggcagcctg
     7981 tgtggggtga ttatcggtgt ggtggtttca ctgcaactta ccccgattat tgagtggatt
     8041 gaaaagttga tcggtcatca gttcctctcc agcgatatct attttattga tttcctgcca
     8101 tcggaattgc actggctgga cgtcttctac gtactggtca cagcattgtt gctgagtctt
     8161 ttggcaagtt ggtatccggc gcggcgcgcc agtaatattg accctgcgcg agtccttagc
     8221 ggccagtaaa ggcagtacat taaaacaagg agcggcaatg tattacgggt ttgatattgg
     8281 tggaacaaaa attgcgcttg gcgtgtttga tagcggtcgg cagttgcagt gggaaaagcg
     8341 ggtgccgaca ccgcgtgaca gctatgacgc atttttagat gcagtgtgtg agctggtagc
     8401 tgaagctgac caacgttttg gctgtaaagg ttctgtcggc atcggtattc cgggaatgcc
     8461 ggaaacagaa gatggtacgc tgtatgccgc caatgtcccc gctgccagcg gtaaaccgct
     8521 gcgtgccgac ctgagcgcac gtcttgatcg cgatgtacgc cttgataacg atgccaactg
     8581 ttttgccctt tcagaagcct gggatgatga atttactcaa tatccactgg tgatggggtt
     8641 gattctcggc accggcgttg gcggcgggct gattttcaac ggcaaaccga ttaccggtaa
     8701 aagctacatt accggcgagt ttggccatat gcgtctgccg gttgatgcgt taaccatgat
     8761 ggggttggat ttcccgttac gccgctgcgg ctgtggtcag catggctgca ttgaaaatta
     8821 tctgtctggt cgcggttttg cgtggctgta tcaacactat tatcatcaac cgttgcaggc
     8881 tcccgaaatt attgcgcttt atgatcaagg cgatgagcag gcaagggcgc acgttgagcg
     8941 ttatctggat ttattagcgg tttgtctggg aaatatcctg accattgttg accctgacct
     9001 ggtcgtcatt ggtggtggct tatcgaattt cccggcaatc acaacgcaac tggcggacag
     9061 gctgcctcgt catctcttac ctgtagctcg tgttccgcgc attgaacgcg cgcgccacgg
     9121 tgatgcggga ggaatgcgtg gtgcggcctt cctacatcta accgattaaa caacagaggt
     9181 tgctatgctg tcgcgtcggg gtcatcggtt aagtcgtttt cgtaaaaata aacgccgcct
     9241 gcgcgagcgt ttgcgtcagc gtattttttt cagagataaa gtggtgccgg aagcaatgga
     9301 aaaaccaaga gtactcgtac tgacaggggc agggatttct gcggaatcag gtattcgtac
     9361 ctttcgcgcc gcagatggcc tgtgggaaga acatcgggtt gaagatgtgg caacgccgga
     9421 aggtttcgat cgcgatcctg aactggtgca agcgttttat aacgcccgtc gtcgacagct
     9481 gcagcagcca gaaattcagc ctaacgccgc gcatcttgcg ctggctaaac tgcaagacgc
     9541 cctcggcgat cgctttttgc tggtaacgca gaatatagac aacctgcatg aacgcgcagg
     9601 taataccaat gtcattcata tgcatgggga attgctgaaa gttcgttgtt cgcaaagtgg
     9661 tcaggttctc gactggaccg gagacgttac gccagaagat aaatgccatt gttgccagtt
     9721 cccggcaccc ttgcgcccgc acgtcgtttg gtttggcgaa atgccactcg gcatggatga
     9781 aatttatatg gcgttgtcga tggctgatat tttcattgcc attggtactt ccgggcatgt
     9841 ttatccggcg gctgggtttg ttcacgaagc gaaactgcat ggcgcgcaca ccgtggaact
     9901 gaatctcgaa ccaagtcagg tcggtaatga atttgccgag aaatattacg gcccggcaag
     9961 ccaggtggtg cctgagtttg ttgaaaagtt gctgaaggga ttataaagca ggaagcattg
    10021 cctgatgcgc tacgcttatc aggcctacaa ggggacgcaa ttaattgaat ttgcgggttt
    10081 tgtgggccgg ataaggcgtt cgcgccccat ccggcataat acacgcgcac ttttccggcc
    10141 tcttttatcg tttactcgtc aataacaatg cggcaataaa cgtacagaca ccaattagcc
    10201 ctgcccagag tggcgatatt ctggtaatcg caaagctatt atcgatatac aaacataaag
    10261 cctgataacc catattggca atagtcatcg ccactgcaaa ttttaacgca taaataaaac
    10321 gagtcgtgta gcaaaagatc caggaaaata agataaacgt cgcagcccag gcgataaaac
    10381 ctaatgtgtt ggtgctgccg gggggagcat tttcatgctg ttgtcctgga tgcggataat
    10441 acgtcagtgg cggcgtcggt tgttttaata tctgtgcttg tagaacagcc aatgcattat
    10501 tgattccctg ataccagaga tctgcattta aataagccag cgtgtgattt tctatcagaa
    10561 ggccgagatg tttatcgtcc agaccacttt caacctggcc gttggtgatc ataattaacc
    10621 gtggttcgcg ggtagtaaat agtaacagaa cacttccttt tacattactc aatgaagatg
    10681 acgcatgcgc cagcatcgct ttaccatttt gtctgagact ggcttcatta ttaatgtcgg
    10741 gtaaaatcac aataaacggt gcaattcccg tttgttggcg aaaaagctcg gctttacgtg
    10801 cgatctcttc agcatccggt acagtaccgg taacatccgt aaaaagtgtc ttcattattg
    10861 gaatcaatgg tttactggtt gctaccagag gcaggagtat cagtaaactg agcagtataa
    10921 tgaatttttt catgggcagg acttccgttt cctgcgagat tttttggtgg aggggagcgt
    10981 gattatcaga ccgacactac cgaaaataat gcctgctatg ccaacaaagc cccatttaaa
    11041 aaaattaacc cagggattgt ttatttcggc ttgttctgga tgccctggcg gataatagac
    11101 attgactctg tcaccttcgc tatcttcata aaaatcacta ccgatattcg aattgaaaat
    11161 tagcgtgtaa tctggcgtag ggcgaaaggc aaccactgga taccaggaac cattatcatc
    11221 gacgtcgttt gagtgattgt tgtaccaaac ggcatcaaca atgattcctt ctgtatgaac
    11281 cgcgcttttg gtaaaagtaa aatggtaata aaagatgatt gcagaaataa cgagcaatat
    11341 cagaccgata ccgagaaaag cgcgggataa cactttactg tcctgactca ttagccacat
    11401 ccttgctaaa taaaaaacgg gcggtaatgc caccgcccgc ttgctgaatt aacgtcctgc
    11461 tttcagcttc tgataatact cttcataaat gctgctggct gcgccaacgt cattctgcca
    11521 ttcaccattt ttaatggttt cagcatccgg gtagagcgtt ttatcgttcg ccacttccgg
    11581 gcttaacagc ttacgcgccg caaggtttgg cgttggataa ccgatagttt cagcaacctg
    11641 ttttgccaca tccgggcgca gcaggaagtt gatcaatttc agcgcgcctt ctttgttttt
    11701 ggcatttgcc gggatcgcca ggctgtccat ccagaaaatg ccgccttctt tcggccacac
    11761 cacgtcaatt ggcgtacccg cctgccgtgc aacgaaagca gaaccgttcc agatcatgcc
    11821 gaggttaact tcgccttcca tgtacgggtt agctgggtta tcggagttaa acgctgcgac
    11881 gtttggcatc agttttttca gctcgttata tgcagcttca atctctttcg gatcggtggt
    11941 gttaccggag tagcccagct tacgcagcgc catctggaac acttcacggg catcgtcggt
    12001 caacagcagg ctgcctttgt actcaggctt ccacagatcg gcccagctgg tgacagattt
    12061 cggatccacc gcatcaccgt taacaccaat cgccgtcgca ccccagatat acggaatgga
    12121 gtaatcgtta ttcgggtcaa acggcttgtt gagcatgtct ggatcgagat tgctgaagtt
    12181 cgttaacttc gacttgtcga tcttctggat catcccttct ttacgcattt tatcgacgta
    12241 ataggttgaa ggaaccacca ggtcataggc accgtctttg tacgttttca gcttggcgta
    12301 catggtttcg ttcgactcgt aagtcgaata gataacctta ataccggttt ctttggtgaa
    12361 ctgttcaagc agtcctggcg gcacgtactc ggtccagttg tagaaataca gcgtgttgtt
    12421 gtcatcggcg tgagcggcgc tcatgcccag tgccagagca cccgccgcga gcaggtggcg
    12481 tgaccatttt ttcatttaac gtcccctgtg ttacctttcg ttttatcacg agcaataagc
    12541 tggctggcaa taaccatcac cagcgacagc accagtaata tggttgccag cgcgttaact
    12601 tccggcgata cgccgacttt gaccatcgaa taaattttta acggcagaat ttcataactt
    12661 ggtccggtga cgaacgaaga aactaccaca tcgtccatcg acagggtaaa gcttaacacc
    12721 cagcccgccg ccaccgcagg cattgccagt ggcagaatga tttttcgcag aatggtaaat
    12781 tcactggcac cgagatcttt cgccgcttcc agcatccgca cgtcaaaacc tttcaggcgc
    12841 gaatacaccg tcaccaccac aaacggcagg cagaaggtga tatgcgagaa cagcagcgac
    12901 cagaagccaa gctgaatacc cagcagcata aacagcacca gcagagaaat cgccatcacg
    12961 atatctggtg acatcatcac cacaaacagc attccgctaa cgaacggctt accacgaaag
    13021 cgataacggt acagcgcaac tgccgtcagt gaaccgataa gcgtagcaaa tgtcgccgaa
    13081 aacaccgcca ttgttagtga atgctgcgct gcctgtaaca ggctgtcgtt gttcatcagc
    13141 aggctatacc atttggtggt aaaaccctgc cagttgatgc caaagcgcga gctgttaaag
    13201 gagttcacaa tcaaaataat gattgggata tacaggtacg cgtagatagc ggtcataaaa
    13261 ccgccgcgaa gcagtcgacc gatcattcga gttccacctt tttattcata aggcgggaca
    13321 ctttcaggat tttgaattta ttcaattttt tcctttctcc aaaatctctc caaaacgttt
    13381 ccccaaaact aggccacgca ttttacaaca cttttttcct gagatggtga cggatattcc
    13441 atatggtgtt tatcaaataa ccaacttaac ccattcctga cctcgagtat cgttatagcg
    13501 atcggtggtt gcctggactt tatgtcctag taatgttttt gtatcaatac cctgtgcacg
    13561 gtacagccgt tctgatagag agcgttgttc atgaaatgtt ggcggagttt ttcctgctgg
    13621 tggaattatc ccagccagat cccgtgcttt ggcaaagtag tcgctcaggt tgtctttact
    13681 catcggcttc ggttgtttct ggtgccgact atggattaga tatggactta atattctgtc
    13741 tcggcaccca tcaataactt cttttaacgt tatcccaatg gcatcacagc gtagtgtaag
    13801 cggtaacgcc agacgcattc cggtttttcc ctgggtgata tgcaagtgtt cgttccacac
    13861 atctgaaaaa cgcatgtggc aaatgtcatc acggcgctga ccagtaacaa tcgcaagaag
    13921 cattgcgtta cggataaagt gtttttcagg cgttgcattg taaatttttt gccagtcttc
    13981 catggtgagc ctggctctgg ttactttagg gatcggttta cgggtagcct ccggaggatt
    14041 ccatccagga ggaacttccc ctgcatgctg tgcttcttta taaatatcaa cccataatcc
    14101 acgatttact ctcgctgtgc tgaccatgtc tttatccagc cactcatcca gtattaatgc
    14161 aaagtctctt acttccagtt ctttcaatgg gtggtttccc agacgggaaa ccaggtatgc
    14221 agccattcgg gttttttctt tgtgagttgt agctgcaata tctccatttt tcagtcgcgt
    14281 gtcctgtatt ttcagatatc gatcaaccca tgcctttaat ctgatacccc gacgttttgt
    14341 tgctgacgga ctttcatcaa ttttgcgcat gaaatattca gcttctgctg cagctattcg
    14401 ctgattggct gtggaagcga ttttttctgc cttacctttg tctgttccga gtccgtgaaa
    14461 ttttccagtc acaggatttt tatactggta gtaaactctg ccagttctgc gatcaaactt
    14521 ttcgtaaaga ccggctacgt cagtgctgtt ttttcgtggc ctcggtgaca tgagttaaaa
    14581 tctccttcag tgcatcatca tcgccagtat gaatttccgg cgcaattccc gtttcaccag
    14641 gcccaacaaa tactgctcgg cgatctatca gccaacgccc acgaattttt tgtggtcttg
    14701 gaacgatgta tcctagtttt ccgtatttca ccagggtagt gtttgttatt gggagactga
    14761 accgttttgg tttccactcg tcgagcgtta tcaggtactg ttcgctcatg gctatcactc
    14821 cggaacgcgc cagttgcaga atatcaacga caactggcga cggttgaaca ttaaaaatca
    14881 gcctgattcg ggatcagttt ttgccagata gctgaaacgt attttgcctg gtgacgagcg
    14941 tcatcaagtg cattatggcg ctcaccttcg aatggaatag ccgttctggc atcga
//
LOCUS       DAZGLJ010000112        14937 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000112 DAZGLJ010000000
VERSION     DAZGLJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.112"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(105..515)
                     /locus_tag="ACHK49_002954"
     CDS             complement(105..515)
                     /locus_tag="ACHK49_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO9263570.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(814..2196)
                     /locus_tag="ACHK49_002955"
     CDS             complement(814..2196)
                     /locus_tag="ACHK49_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO9263571.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(2206..3156)
                     /locus_tag="ACHK49_002956"
     CDS             complement(2206..3156)
                     /locus_tag="ACHK49_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO9263572.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(3299..4717)
                     /locus_tag="ACHK49_002957"
     CDS             complement(3299..4717)
                     /locus_tag="ACHK49_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO9263573.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEKQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(4717..6264)
                     /gene="fdrA"
                     /locus_tag="ACHK49_002958"
     CDS             complement(4717..6264)
                     /gene="fdrA"
                     /locus_tag="ACHK49_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO9263574.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(6254..7117)
                     /locus_tag="ACHK49_002959"
     CDS             complement(6254..7117)
                     /locus_tag="ACHK49_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO9263575.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(7157..7762)
                     /locus_tag="ACHK49_002960"
     CDS             complement(7157..7762)
                     /locus_tag="ACHK49_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO9263576.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELAREQGFEEIAQLLIAAGA"
     gene            8020..8517
                     /locus_tag="ACHK49_002961"
     CDS             8020..8517
                     /locus_tag="ACHK49_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO9263577.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            8609..9541
                     /locus_tag="ACHK49_002962"
     CDS             8609..9541
                     /locus_tag="ACHK49_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9263578.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(9583..10671)
                     /locus_tag="ACHK49_002963"
     CDS             complement(9583..10671)
                     /locus_tag="ACHK49_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO9263579.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(10813..14796)
                     /gene="ehaA"
                     /locus_tag="ACHK49_002964"
     CDS             complement(10813..14796)
                     /gene="ehaA"
                     /locus_tag="ACHK49_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFO9263580.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITKNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSQVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSAIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSTEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT         3454 a         4054 c         3516 g         3913 t
ORIGIN      
        1 gcagcaacaa taagtgtgac attcatcacg taaaaaatag caaaataaac aaaattagcg
       61 atattttatg tgtgtgagtt tatttcgcgt agataaatag ttatctacac aacgtcaata
      121 agggcatatt cttcaaatat aacgtccatt tctggtgaat agcttccttc tgcctggaaa
      181 aaacgacggt gttcattacg ccaatattca agactgaggt caccttcacc ttccttacgg
      241 gcaagctcca cggtgacatc agaaaagcgc agtaaatgca ggccaatcac tctgataaca
      301 catacaggct caccacggct attttctaca atcttatact caccaatcat cggaaatgcg
      361 ttgtcttcaa tacatcccgc cagcgaacca caggaagctg ttttaacacc agtcgcaatc
      421 aacgttgcga ggtgatcggc caacgctgct gaatcgccaa aagtacaaaa ggaagcatgg
      481 ggatattttt gtgagaattt ttcttttatg gtcatggcaa tatttttgcg attacggaaa
      541 aacaaatcac atcttaatta ctgttggtaa ccatcagcaa gagtatcatt ggtttttcga
      601 tactgaatct tttttatccg aagcaggtca catttatggc ctgataaata cctttgttaa
      661 aactcacttt tgtgtatttc tctcaaataa tcccctggaa attatgccgc gagattataa
      721 agttcaggat cattcattag caaaccattc ccgccccact ccctggggct taaatctctt
      781 acaattcacc gttgcaggcg tttcaaatgc gcatcaacct gccacactcc cccacaccag
      841 ttgcccttta tggaacgttg cggttctcgg cgagatacgc gccaccgcct cggcggaaca
      901 ggaggcgtcc accagcacaa agctggcatc atcctgcgct tttggccata cacgctcgcc
      961 tttttcattc agcggcaata catcgccagt ggctagaaac agcgaacggg agaggttctg
     1021 ttcgttagga cgaatataga gctgcgcgta gagattggct ttttccagca tgtcgcccag
     1081 accataaggc gaccagtggt cgataacgct gtcagtgcca gtcatcactt ttacgccttt
     1141 gtcgtgcaac tgtttgagcg gcatatgcag cgtgccaatc ggcaccgtcg aggcgataga
     1201 aatttgctgc gccgccatcc gattcgccag cgcatcgacc tgttgttcgt tgagcgtagc
     1261 cagcgcaaag gcgtggctga tggtcagctt acctttcagt tgcggcgttt tctctaccgt
     1321 ttcaaccata taattgatgg ctgccacgcc cgacggagtg gtttcgtgca ggtgaatatc
     1381 gacacctttg tcgtagtcca gcgcaatctg aaacatggtg tcgagggatt tttccatcgc
     1441 gccatcgaca ctggtcgggt ccagcccgcc gacgtaatgc gcacccgcct gcatcgcttc
     1501 gcgcattaag ggttccgatt tcgacagcag caaaccgtgc tgcgggaagg cgacgatttc
     1561 acactcgaag cccgcctgac gtcgcgccag caccgcctgc aaattctgca gatttttcag
     1621 gccggaaacc ggttcgatat tgcagtggct gcgggcaatg gtggtgcctt tcgactgcaa
     1681 tagatcgatc agtttttctg cccgttcctg cgtgtacggt tgcagttccg gcagcatttt
     1741 ctgctcaagc ttgatcatgt cctggatagt ggtgcctgcc ggacgattga gcgagcgcca
     1801 cggcccgccg tagaaggttt tgtcgagatg aatatgcatg tcgcgagtgg tgggtagcat
     1861 caacttaccg ccagcgtcat agtgcggcag cgtggcgtcc ggatgctgct tgttctcgcg
     1921 cagggcgaca attttgccgt cctgaatctc cacggtctgg cgcgccgtgc gggtctgcac
     1981 cgccacaccg ttttcgtagt caaaaccggt ttccagcagc acgttatcga gatagtaatg
     2041 cgggtcagtg attttcatgg tgttgttcgc ttcgcagttt agggtgccag ctaccgccgc
     2101 atgggcgagc ggcacagagg taccaaacag cgcggcggcg gtgaccatct taccgctctg
     2161 gctcagaaat tcacggcggc tattgctttc tttcatccta cgtccttacg ttttaataat
     2221 atgggtgccc gtttcaccgc gcagcgccgc aggcaggcat tccggcgtgg tgataatcac
     2281 ttctttgccg ccctgctcca ggaacgtcag gctggcgatg atttttggca acatgctgcc
     2341 gggcggaaaa tgcccttcct gcatatagcg agtcatggtg gcaatatcca cccgatccag
     2401 cgcctgctgc tgcggtttac caaagtggat acagactttt tcgacgccgg tggtgatcac
     2461 cagaatgtcg gcgtgaattt cacgggccag cagcgcggta gagagatctt tgtcaataac
     2521 cgcgtccacg ctttggtaat cccccgcttc agtacgcact accggaattc caccgccgcc
     2581 cgcgccaatt acgacaaaac cttgttggat cagcgcctta atggcgggtg cttcgacaat
     2641 acgtttcggt tccggcgagg cgaccacgcg gcgatagccc cgtccggcat cttcaacaaa
     2701 acaccagtca gggtttgcct tttgtagttt gtcacgctgg ctttcactga agaatgcacc
     2761 aatgggcttg gtgggatggg caaaacctgg atcgttttta tccacttcca cctgagttac
     2821 cacggtgacg gcttttttct cgccgtgacg cgccagccgg ttattcagcg cctgttggat
     2881 cagatagccg atgccgccct gcgtatctgc cacacagttc gccagcggcg ttaagggcag
     2941 cccttcgcgc tcgtgggcaa tctccgcacg gcgtaaatcc agcccgacct gcggcccgtt
     3001 accgtgggtc agcacaatgt cgtaatcgga agccagcatt tccaacaccg tatcggccac
     3061 ggctttcacc gcctccgcct gatgctcaat cgactggctg gcgttatctt tgataatgct
     3121 gttgccacca atggcaacga ccacaagctc tttcatgttg ttatgtccgc tacagagggt
     3181 gattaggtta tgaccaacgc cagactgcct gatgcgctac gcttatcagg cctacaattc
     3241 tcgggccatc cgtaggccgg ataaggcgtt cacgccgcat ccggcatgaa catgtaggtc
     3301 agacgccgta acgttcgcac cagccaagaa tggctttctc gaagcaggca aaaggtggat
     3361 gcacaatgcc cgcgccaatc atgccgacgc ccgcatcttt atgggcgatg gcagtgttga
     3421 tcaccggcag aatgccgctg ctacccacgc gggtgatgtc gattgccgac ggcacgccca
     3481 taaaaccgag cagcggaatg gtgacgttgg ggttttcacc gagagtgatt tcacgcatct
     3541 ggcgagagaa atcgatagcc tcttccaccg tgccgcccac cagcgcaacg attgcaggcg
     3601 cggtcgccat agcaaatccg ccaataccgt aggtttcagt gatggcgctg tcgccgatat
     3661 ccagccccga atcttccggc ttataaccgg caaacatcgg gccgatgacc tgctgcgccg
     3721 gaccggtaaa ccattgcccc ggtaatccgc tgacccgcag gccgaactcg acgccgttac
     3781 gcgccatggt ggtgaccacg gtgctgtatt cgatgccgtg cgccgcatcc atcgccgctt
     3841 tacacatcgc catccacgtc gggccggaga agtagtcgct gctagcgaca aactcaaaca
     3901 cttcgcgctg tttctccacg gaataacctg cctgaataat tcccggcgtc agcgcctgaa
     3961 tcagcaacgt cgtcccggcg ttattgcggt tatggcactc atcgcccata tgcagcgcct
     4021 gcgccagcat taagcgcaga tcaatctcgc cgataatttt catcgcgtcg cgcagcattg
     4081 gcccctgcac atcgcgcatc cagttgaggc ggtcgatcac gctctgatcg ttggctccca
     4141 tacgcaaaat cttcgccatc tgctcgctca tattggtgta agcgatgttg ccgtaggttt
     4201 tgttttttac gatgtgcata aacatcgagg cagaggtaac acccgccatc gatcccacac
     4261 agtcgtgttc gtggcacggc gagaaggtga tctccccgga ggccgccagt tcagccgcct
     4321 cgtcgagatc tttcgccagc ccttcgaaca ccaacgcccc ggtgaccgcg cctttcatcg
     4381 cgccgcacat tttttcccag gtgatcggcg gcccggcgtg aagaatggtt ttcggcgtca
     4441 tgcccggcac cacgttaatc gcctgatcaa aaccaatcag caccggatgc gactggataa
     4501 tgcgctccag cgcctgctgg ttagcggcgg cgattttgtc tgccagcggc gaatcggcaa
     4561 tgttatccag cgcctgcacc acctgcatat tgccctgccc cggcggcgtc cagtcgagtt
     4621 gagtcacttc tacatgctgc tttttcaggt catcgctaaa catggcgatg ccgacgttaa
     4681 taacgttcaa tggttggcta aacagtgact ggctcatcag gcttcctccc ctttgcagat
     4741 aaattcacgc gccagcaatc cggtattggt gctgctgctc gccagaatca ctccggcatc
     4801 aagcagcatc tggctttgtt gttctaacga tggcgtatca agatcggtac ccagcacata
     4861 ggcgagaatg atcaactcgc gtccttcggc agcggcgatc gctttcgctt ctttgatcgc
     4921 ctcgatggtg gagccgaccg gatcttcatg cgatccaaat ccgagcacaa aatccatcac
     4981 gatcaccgcc acttctggat cgcgcgcctc ttcgatcaag cgactgatgc ggttggtggg
     5041 gtcaatcatt ggatgcggct tgccgttcgt gaagtcgtca tcgccaaagt cgaggaaggt
     5101 gtgtttgatg ctgcggttga tatcttgcag gcggaattcc ggatcgggct gaatgttgct
     5161 gtagacatcg ccatgttttt ccatcaccgc gaacatggtt tcgtcgcaca gcgtgccgcc
     5221 gcagaacagg ccacgaatgt atttctgctg cggttgcaga cgcgcacgca catcagcaat
     5281 caacggctgg ttaagcgtat gcaggtcgag attttcctgt ttcacgccgg agagcatcac
     5341 cgcttttagc gccgcctctt tgctgccgcg ggcaaactgt agcccctgct catccaccgg
     5401 cgtttcaacg cgaccgagga agcagaccac caccggcttg cggcaggcac gtgcgcgttc
     5461 cagcactttg cgggccaccg caggcgcggg aggttttgaa atcagtgcga taatttcagt
     5521 ttgcggatcg ttttccagca tcccgatggc gtcgagcatc atcaggccgc cgattttctc
     5581 gctcaggtcg cgcccgccgg tgccaatcag ttgtgaaaca ccgccaccaa attcatgaat
     5641 gcggacgctc agctcctggc tgccggtgcc ggatgcgcca acaataccga tattgccgcg
     5701 acgcacggcg ttaccaaaac agagcgccgc gccgttaata atcgccgtgc cgcagtccgg
     5761 ccccatcatc agcagccctt tttcgtgggc caactgcttg agcgccagtt catcttcaac
     5821 tgagacgtta tcggaaaaca gcatcacgtt gagatcgttt tgcagcgcct gacgcgcttc
     5881 gcgagcggca aacagaccgt tgaccgaaat caccgccagg ttactttccg ggatatgctt
     5941 tttggcgctg gcaatagtgg cgtaacgcgc ctcgtgcgag ccgctttgcg ctttggtgtt
     6001 gaacagttct tcaatctcca ccagcaactg ctcgttgtcc gcacccgatt taccattgat
     6061 gacaatcatc aggtcgccgt ttttcgcctg ttccagctcc ggcgtcagca gtcctaaatt
     6121 tttcagtaca cctttgttca tttcggtcgc catcgccaca aatgcctgct cgacgccgtc
     6181 gagtttattt gcacgcgtgg agatagacat cagcgagaca gaatcaaaat aggtattcgg
     6241 tttaatgact attttaactg acataattcc ctccgtaggt ttggctcagc gcacaaccat
     6301 cggccatgcc atacagcatg tcgcagccgg aactggagcc aatatgttta attttttcta
     6361 ttgcctgagt tgcgttccca gggatgtcat aaataatgtt gtgaataaaa cgatgaatat
     6421 tttcccggca acgttgttgt aatgcggctt ccagcgttat agcacttaat aatgtggtgt
     6481 tatttctgcc gcgctgcata ccgagataaa attcctcttt gtatttttcc gacggatgcc
     6541 cgggaataaa taaaataagc gccagacctg ttaaataatc gtcggctgat ggcgtaaggc
     6601 caatccctaa cccaatcata ctggcgaccg ctgcggcgat attttgtttt tcgtttactg
     6661 cctgaataac tgcccgtcgt ttaatatgta gttctttatt taacgcctga taaaacggat
     6721 tatcgccgcg ataattaaac agggtttcat tttcccccag gtgctgatga ataatgtcgt
     6781 gccactgtag ccaggcgata aggtgaaaat tcacagcggt catttgcaag gttggggaca
     6841 gccaacgccg acaacgactc atctctatat gaagatgttg accaaccgta ataccttgat
     6901 cgtcaaactg aaccttatca ccatgccgga acagatcatc aaagtgagtg agtgccaacc
     6961 gacaactgtt tggcgcattg tcgtactctt cacaaagcag cgtcagcaac tgctggcgag
     7021 ccgggagtgc gatatttacc gctctggcaa agacctgttc aacctgtcct tgcccccgct
     7081 gcgccagaaa atccgcatcc gcagttagcg cccacacaca ccgtctttct ggtgtgttga
     7141 tagcaagcct cccggtttat gcacctgcgg caatcagtaa ctgcgcaatc tcttcaaagc
     7201 cctgttcccg cgccagttcc agtggcgttt tgccatattt atcggtcaga tgcgggctgg
     7261 caccgtgttc cagcaataac tgcacaatcg cctgctgttt aataccacca tcattaagca
     7321 caatcgcttc cagtagcggc gtccagccta catggttggt ctggttaacg ttaatctccg
     7381 tatgcgccaa aagctctttt acaatactta aatggccttt ttcacaggca ggcgtcaggc
     7441 cgacaccgcc aaaacgggtt acgcaattaa gatcgggttt agccggtaaa ataattcgta
     7501 gtagcgttaa atcatcgttc agacagctaa ttaaaaaagg atttaaacag gtatgatctt
     7561 gcttattaat atccgctccg gcatcaatta atgcctgaac gcaagcatat tgctgatata
     7621 aacttgccag cgtaattgcc gttttcccct gacgatcgca ggtattaata tcgacaccca
     7681 gcgcaaggca ggtttttact ttatcgatat caccctgttg tgcagccaat aaatagtcgg
     7741 caggtagatt ttttatactc attcacattc ccctggtttt tgtggctgtt ttcgatgaat
     7801 tgagaatagc aacgataatc tgataaaaga acccgcttaa atttaattcg ctgtatttcg
     7861 cctttttgtt gaatgatgtt caacaatctt ttcgcagtgt gcggtagtgc aatatttgtt
     7921 tctttgaccc gatacccttt cactcaatca ggttttattt ctctatagtc tgcacatgta
     7981 agagtagctt tcgtccatgt ctaaccagag gggaacaaga tgaacggact cacggcaacg
     8041 ggtgtcaccg tcggtatttg tgcagggctt tggcagttag tgtcttcaca tgtcgggctc
     8101 tctcagggat gggaattatt aggaaccatt ggcttcgtcg ccttttgcag tttttatgcc
     8161 gcaggcggcg gtaaatcggg gtttatcaga agtctggcgg ttaattattc cgggatggtg
     8221 tgggcatttt tcgccgcgct ggctgccggt tggctggcct ctgtaagtgg actgtccgcg
     8281 ttctgggcga gcgtaattac taccgtaccc ttctcggcgg tagtggtctg gcaagggcgt
     8341 ttctggttac tgtcctttat tcccggtggc tttctcggca tgacgctgtt ttttgccagc
     8401 gggatgaact ggacggtgac gttactgggt tttctggcgg gtaactgcgt ggggataatt
     8461 tcagaatatg gtggacaaaa attaagcgaa gcgacgacta aacgcgacgg gtattaaaag
     8521 ttattcaaaa atgattatct atctttaacc gatacctcta cagtcatagc tggagaaatt
     8581 catagtagat aatggcgaga atggtttaat gaactcaatt tttaccgaag agaatctgtt
     8641 ggcatttact accgctgcgc gttttggcag cttcagtaaa gccgcagaag agttgggttt
     8701 aaccacttcc gccattagct acaccatcaa gcgtatggag acggggctgg atgtggtgct
     8761 gttcactcgc agtacccgca gcattgagtt aacggagtcc gggcgttatt ttttccgcaa
     8821 agctaccgac ctgctgaatg atttttatgc catcaaacgc agtatcgata ctatttcgca
     8881 gggcatagaa gcgcgtgtgc gcatttgtat taatcagctg ctttatacgc ccaaacacac
     8941 cgcacgcttg ttacaggtgc tgaaaaagca gtttcctacc tgccagatta ccgtcaccac
     9001 cgaagtgtat aacggcgtct gggatgcgat tattaataat caggccaata tcgccatcgg
     9061 cgcacccgat acactactgg acggcggcgg tattgattac actgaaattg gcgcgattcg
     9121 ctgggctttt gctatcgccc cggatcaccc gttagccttt gtgccggaac ccatcgccga
     9181 aagccaactg cgtctctacc ctaatattat ggtggaggac accgcgcata cgattaataa
     9241 aaaagtgggc tggttgctgc acgggcagga gtcaattctg gtgccagatt tcaacaccaa
     9301 atgtcagtgc cagattttag gtgaaggaat tggttttttg ccggattaca tggtccgtga
     9361 ggcgatgacg caatccctgc tggttacccg acaaatccat aatccgcgcc aggattcgcg
     9421 catgttactg gcgactcagc attctgctac gggtcaggtg acgcagtgga ttaaaaaaca
     9481 atttgcgcca aatggcatat taacggggat ctatcaggat ttactgcatc gggagaatta
     9541 atcgctgcca acctcccgat gcgctgtgaa tagcggttta catcaaccac ctgctttcat
     9601 tacccattca gagataaatt tattacccgg tactggcgga gagtacaaat agccctgcaa
     9661 aaagtgaacg cctttaccga tcattaaatc cgcctgctcc tgggtttcta ccccttccgc
     9721 cacgatactc agaccaggct tacgcgctag ttcgacaata ttgtccacaa tatgaccgga
     9781 gatttcgtcg acactcgcca tttgcacaaa tgacttatcg atcttaataa aatcgaccgg
     9841 gaacgcctgc aagtaacgat aggtcgcata acccgtacca aagtcatcca gcgcaaaggt
     9901 aatgttgtgc tgatgaaggc tgtcaaatat cgctctggct tctggcgtta ccggaatagg
     9961 gttacgttcc gttagctcga gaaccagctt gattttatcg ttacctaatt tcttaaccag
    10021 gttcagacac tctttttcaa atcccgctgc caaaaaacaa cccgccgaga cgttgatgcc
    10081 aatatggaaa ttgtccggca gcaaatgttt taccggcatc agaatatccg cagtctgttt
    10141 catcagttgg cgggtcatta tgacaataag accggatgac tccgccagag gaataaactg
    10201 atccggtggg ataattcccg tttgtggatg ttcccagcgg acaagtacct cacagcccgt
    10261 cagtacccca gtctgcgcgc agaaaaccgg ttggatccac ggtttgaatt cgtggttttc
    10321 caacgccaga ctgatggctt caggcgtgac gatatggtga caacggttat cagggatata
    10381 aaaattattt ttatttcccg tgccactgat gacttgcgga tgatattgaa aaaagatatc
    10441 ccgccagaag gtaatatcac tctggatgcc tagtttttca tacagctgct tcttttgatg
    10501 tgaaattgtc tttgcactgc gatttctgta ctgtgagatt tctgtaaccg acattccctc
    10561 cattagcagc ttgagtaaaa gccgttcttt ttcggacacg ataccaggca acgtcaaatt
    10621 caccgttgcc ccgaactctt ccaaaaaaac actcaaatca tgcgaagcca ttgaacacac
    10681 cttgattaaa tcttgtgcgc cagctaaatc tgcgagtctt cactctgact gataaagctg
    10741 gctgcaagaa aatgacacag agagccgaat tatctcccgt tcggctctct ctccttttac
    10801 tcattattca gatcagaatt gccacttaat gccaaccatt gcagaggtgt cattatatcc
    10861 cctatcggca acctgaacgc ccacattccc ccagacattc aggttggcat ttagctgtcc
    10921 ttccaccccg gtttttatct cagcaatatt tcgggctcca tcctgagtga cggacacgcc
    10981 atccatactg gtgctgaaat ccttactgtt atgcagccag ttcacttcta caaacggctg
    11041 gaactcgcgg gatttaccgt catccatttt atggtggctc ttcagccagg tttttacgcc
    11101 aagtcgggtt tgaacattgc catcaccgtt gctatgaacg agggttccgt tgctttcgcg
    11161 gtgcttatcg gctttgactc ccatccaggt aacctgtgct tgcggctgaa cataccattc
    11221 attacgcgtt ccctggctgc cattaaattc agctaatttg tgtttgtatc cagcttccag
    11281 tgaagcggta aatccttttg atttatagga ttcactttgt aagtcatccc ctttcactgt
    11341 gttatcaaac cagctgtact gcgcccaact gtcgagatac gcgccattac gcgactcgtc
    11401 atcggcatac caggtggcat agaggccggt gctatatccg ttcacactcg cttttgcacg
    11461 ataaccggtt cgcgaggaaa tggttttgct gtcgctgttg ccatatcccg ccatgacccc
    11521 aacatgccag ctgtcgctgc cgttttggct ccactgcgcg acatcgcctc ccagttgcag
    11581 aacatagcga ttgctttggg ttttcagctg gccgctgcca tcacgccatt tattatgacc
    11641 accttcatgg cgcatccaca tagtggtttg tttctgctca cccgtcacca tgtcggtata
    11701 gtacgtatta cccagacgct catgcagacg cgtggtgaac attgtattcg ctgctgcaag
    11761 gttcgcaatg tagctacccg cctccggtcg caggtcatta tccacattca gatccggacc
    11821 cggcgtaggt gtcgggttag ggttcggctc tggattcggg tttggctccg gattcggcat
    11881 cgggtctggc tccggctgca gttcaggaga atcactgccg ctggtcagat accagttacc
    11941 actatttgcc ccttgtccac gcgcgagagt gtagtcatac gcccccgcaa cgatacgacc
    12001 agcctgaaca aattcgccct cagacttacc gtctacatgg ataagttcga taccgttaag
    12061 ggttttcgca cctgtaccgc cagcgttatt caccgtaacg gcagtcgtgc cgctagtatc
    12121 gccctcgaca accaatttat cggttactga gtcatcgcca ttcagtaccg tattcattac
    12181 tagttgaccg ttattaccgt ggtagttccc cttcaccact aacacattgc cagcttgctg
    12241 tcctgacatt ccggtcatca gcgtaccgtt gttggtcaga ttgccgccca gggtgtaatt
    12301 cacagacgca gccgctcttt tttcagtgct attattcaac gccagggtac cgttgttctc
    12361 aacgtttgcg ctacccaatc cctgcgcatg acttaccagg acattagccg aattagcaat
    12421 gttcacattc gccgtcaggt tgctgttatc gcccgcgatg tccagcgtgc cgccctgaat
    12481 attcagctct ccgctccccg ttaacgaacc ggttgaagtg cccccgttgg taaccgtcag
    12541 actgccgcca tttaagctca gcagtgaatc ggcgctgcca ttgagcgtgc ccacggtctg
    12601 gctgtgacca ttcatatcca gttcagtctc tgccgccaga cgcagttcat gggtattacc
    12661 caacacgtta tcgttattca acaaaagcgt cccactgcgc agatcggtaa caccggtata
    12721 gtcgttgtct ttgttagaaa gcgatacggt ctgacccttc tggctgctga atgccaggtc
    12781 accactgccg gtaatctttg cgctgagatc ggcggcattc tcgcttttac cgttagagct
    12841 cagcaccaga gcatcgctgt cggtagcgtg taaatcaagc tgggtcaggc cgtaaccgat
    12901 atacagaccg tcattgttta ccccactcgt cagacgatag tcgtaagtgc cgacggccgc
    12961 tttttgccca ttctgctgaa tatcacgttg ggcgctgctg gagaggattt gcccattttt
    13021 atcctgcaat tgcagattgc ccgcatcgcc cagaacttcc gcaccttgcg catcaaccag
    13081 cttaatggcg ctgttaccat catcgacttc agtgagcgat aacgcagagt caatatcacg
    13141 agatactgag cggacaacgt cactgtcaga aacctgaatc acaccctcgc cgcgcaaatc
    13201 cagcgtttta ctaacgttga ccgtcccttc tgtcatctgt gcacctgctg tcagggcacc
    13261 aaaatcaacg gttccgccgg agaatcccaa agtgctaatg gagtgagcgc ccttacccac
    13321 tgttacctgg cttccctgac ccgcagtgat cccgcctctc tgcagaaact cagcattctt
    13381 ttccaggtta aatgtcgtgt tctggaacgc cagtgaaccc tggaaccagt ctgtataagc
    13441 aaactcatcg aaggagaatt cgtgattccc catgtttatt tcaacaatac ctctaccttg
    13501 aagatcgtta tcaaataccc aatctttatt ggcattgatt tgcagtttgc catcaacatc
    13561 caggtccgca cggccgatgt tcttgtcttc agaaataacc gcgacagcac cagaagcgac
    13621 atcaatatat ccgctgaatg catagttatt gccggtcagt gccactgtcc cactgtcgat
    13681 acttattgta ccattgccgg aaatcttatt ggcaaaggtc atgctatcgt cagccttcag
    13741 gtgtaatttc ccgcggttgt tgatctctgc tgtacctata tttttggtga tccctaaagc
    13801 gccattttgc tcaattttga ctttaccact aaaggcagag ttatcaccgg ttaagtaagt
    13861 cgtgccattg agcgcattga tatagcccag gccctcgctg ccactgacaa tatccgcgga
    13921 aatagtcgcg tttttatccg tgtggtttaa ggtgattttc ttatcggatg tctttgccca
    13981 cacaaactca attttttcgg catcaataat ccctgcagcc ttcgcagcac ttccttcctg
    14041 tgcacctaac gctaactcag agttggtgct taaactaatt ttcggtgcgg atattttacc
    14101 tccatctgat acgactaatg ttgtatcaga acgttggtcg taataaccca ggctaatatg
    14161 ttcacgagca ttaacttcac caccgttatt aaccgacagg aactgattac cgccatatcc
    14221 gccgtaaacg cccataaaga aggtccctgc atttacctga ctaccttcgc cgcttacatt
    14281 cccctgcgta taagtcagac gactggtgcc cttaacatga attttgccgc cattggagac
    14341 attaaaatag ccttgatcat gtctcatgcc tgagtttgac tcatacaaat catcagcata
    14401 taactcgctg ccagcattgg agacattcag gaaccccgta cctttcagca aaaagttaag
    14461 cgggccattg attgttacct ttcctccggt aatatccaac ccaccaccag aagcgagagt
    14521 cagacgcgga cgggaagtat ctgtcgtatt gattaccaga ttggcattgt cagtcaccgt
    14581 aatacggtaa tcgatgctct ggttctcttt gccaaaatca atagttttat cctggtttac
    14641 gatgacatca tcggcgaata ccagagatga atataaactt atcgcgctaa ttaaaacagc
    14701 ccgggacttt tttcctttta ctctgctccc cagctcagag cagacgctcc aacagttacg
    14761 ggacctgttc cactttagcc gatatatttt attcatcaca atctccagca atacatccaa
    14821 ataaaaccaa gattgcataa acagcaacgc attaaatgcc attctggtta ctgttcatcg
    14881 catataaaaa ttcaaaaatt ttgcatagca ttggcaacca catatctgag tatgtag
//
LOCUS       DAZGLJ010000113        14790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000113 DAZGLJ010000000
VERSION     DAZGLJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.113"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1477)
                     /locus_tag="ACHK49_002965"
     CDS             complement(<1..1477)
                     /locus_tag="ACHK49_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO9263581.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDATVTGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKATHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTYRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPY"
     gene            1632..2468
                     /locus_tag="ACHK49_002966"
     CDS             1632..2468
                     /locus_tag="ACHK49_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO9263582.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVIGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDRQKITSLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            2492..3544
                     /locus_tag="ACHK49_002967"
     CDS             2492..3544
                     /locus_tag="ACHK49_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO9263583.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKRINVIGVTDQKGEKILLDTTGPIARTNISYDGIKRRNPNNVIDMK
                     SRQYQCEQVNYDTFISYPQLDTWAAHSDFQSRISTQIARQVALDRIMIGFNGTSHAYE
                     SDFHTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            3590..4390
                     /gene="gpM"
                     /locus_tag="ACHK49_002968"
     CDS             3590..4390
                     /gene="gpM"
                     /locus_tag="ACHK49_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO9263584.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAACTAHEEVLYRLRLAQA
                     RLKAIQARSAKAAIKKELLPDFSGWIEGTLEADCGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLWEI
                     VANQDMPDEVRAKLCKACAFARRGLTDADNMASSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKSKKTTRKPATRKTTATQKAKRG"
     gene            4492..4986
                     /locus_tag="ACHK49_002969"
     CDS             4492..4986
                     /locus_tag="ACHK49_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO9263585.1"
                     /translation="MSMVARTNPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLTGRVTTSRLLHTATGAIAHVTRELEAWQQEQQAAGYQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILGRPRMF
                     AELC"
     gene            4986..5186
                     /locus_tag="ACHK49_002970"
     CDS             4986..5186
                     /locus_tag="ACHK49_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO9263586.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            5189..5512
                     /locus_tag="ACHK49_002971"
     CDS             5189..5512
                     /locus_tag="ACHK49_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO9263587.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGIPEDAVTFFGASIGFMGAEKARDKVIAIFDRRV
                     KERNE"
     gene            5509..5901
                     /locus_tag="ACHK49_002972"
     CDS             5509..5901
                     /locus_tag="ACHK49_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO9263588.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVTTGKSQTMNSRHLTGHAVDVVAYVGSQVSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            5898..6305
                     /locus_tag="ACHK49_002973"
     CDS             5898..6305
                     /locus_tag="ACHK49_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFO9263589.1"
                     /translation="MKLWPTLGVAFLLIAAWGTSMRLSWSLGRENARNEAQASTLKST
                     VDTLNIISAGVQDMQQVLAQLRADNQQRNQDGEVRREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHAGNASPLP"
     gene            6444..8321
                     /locus_tag="ACHK49_002974"
     CDS             6444..8321
                     /locus_tag="ACHK49_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO9263590.1"
                     /translation="MTSKNFALITAMTQAELTQKVNEHLAKGWHLQGETRVAYEPGTP
                     WYLMQAMVADGTTDISPDSPQHGSVPEWYYVVVLAGQSNAMSYGEGMPLPDSYDAPHP
                     RIKQLARRNTVTPGGKACAFNDIIPADHCLHDVQDMSALNHPNADLSKGQYGCVGQGL
                     HIAKRLLPYIPQNAGILLVPCCRGGSAFTQGAEGTFSESTGASQDSARWGVGKPLYQD
                     LILRTKAALQKNPKNMLLAVCWMQGEFDMSAATYSQQPPLFAAMLKQFRADITEFNTQ
                     CHGGRAASVPWICGDTTYYWKNTYGTQYDTIYGAYKNRESDNVFFVPFLTDGSGNNTS
                     TNAPTEDPDAASEGYYGSASRTNKNWVSSNRQTHFSSWARRSIIPDRMATAILNVAGR
                     TLAFISGKAPEIKPSPGGDTPSGPSEDASVRTISLLPTAGDAAAQGWSIKNGGIQLSE
                     GVFKITKQSNKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVA
                     LPDVVAMTGTGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWQTLELVFT
                     ASSATVTPKLNGVAGPAFQVIKDSLTLGLNALTLTDITKNATYGVEIESLVLEINSPA
                     A"
     gene            8345..8812
                     /locus_tag="ACHK49_002975"
     CDS             8345..8812
                     /locus_tag="ACHK49_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9263591.1"
                     /translation="MLKTNSLRKGMIHGCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLTLRERVRITRSSQGAPQAEHLQEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            8805..9440
                     /locus_tag="ACHK49_002976"
     CDS             8805..9440
                     /locus_tag="ACHK49_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO9263592.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            9452..10018
                     /locus_tag="ACHK49_002977"
     CDS             9452..10018
                     /locus_tag="ACHK49_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO9263593.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWCVRVRSGELETGWLRWNTTRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDANPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTDLLRTATLDVTKGGKMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            10015..10365
                     /locus_tag="ACHK49_002978"
     CDS             10015..10365
                     /locus_tag="ACHK49_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO9263594.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            10369..11265
                     /locus_tag="ACHK49_002979"
     CDS             10369..11265
                     /locus_tag="ACHK49_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO9263595.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTTAFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLT
                     ITPATDTADAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            11258..11788
                     /locus_tag="ACHK49_002980"
     CDS             11258..11788
                     /locus_tag="ACHK49_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO9263596.1"
                     /translation="MSNSLLPPSASSFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPDD"
     gene            11791..12549
                     /locus_tag="ACHK49_002981"
                     /pseudo
     CDS             11791..12549
                     /locus_tag="ACHK49_002981"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            13030..13923
                     /locus_tag="ACHK49_002982"
     CDS             13030..13923
                     /locus_tag="ACHK49_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9263597.1"
                     /translation="MSSKGRVTALTDNTQGATGLELYEVYNNGYPTAYGNIIHLKGMT
                     AVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVGAPIP
                     WPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPTSGRAVLSQEQ
                     DGIKSHTHSASASSTDLGTKTTSSFDYGTKTTSSFDYGTKTTNSAGNHSHNIPVGHTG
                     AGNGVSAGFNAALGTGTTSSAGEHAHNVYIGAHNHTIGIGAHAHSVIIGPHGHTITVN
                     ATGNEENTVKNIAFNYIVRLA"
     gene            13923..14501
                     /gene="tfaQ"
                     /locus_tag="ACHK49_002983"
     CDS             13923..14501
                     /gene="tfaQ"
                     /locus_tag="ACHK49_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO9263598.1"
                     /translation="MTFRMSEHSRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDSLFISELGPLPENVTWLSP
                     YGEYQKWNGTSWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLDIATEEE
                     ASLLAAWKTYRVLLNRVNTAVAADVEWPVAPQ"
     gene            complement(14545..>14790)
                     /locus_tag="ACHK49_002984"
     CDS             complement(14545..>14790)
                     /locus_tag="ACHK49_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFO9263599.1"
                     /translation="VDAGENLVLRQNTTIGQIDGDMPGSRVKIGSNVDIGANCCIIGL
                     SRKIGDNVKIGAMSFINKDIPSNCTYITKKSGAVLYK"
BASE COUNT         3679 a         3954 c         4081 g         3076 t
ORIGIN      
        1 tgtacggaat agcagcaggg aaaaaccgtt tcactatctg ataaacagcc tccccgttcc
       61 cgcccgtcac atcaatgccg atgtgttcca cgttgtagcg atacgtgaac tcttcaatga
      121 ctctggcctg ttcttcaaac tccagcccct gaacgcgtcg cgtctccacc gttcgaaaac
      181 ggccaccagg aacagccgga ggaaccacca cggacacagc gccgctgtcg ccgttgccac
      241 tactgccgtt tgcgtcatac ccaatccata ccggacgatt ccccatcggg cggggagcaa
      301 aaggtttcca gtctttccag tcgtcgtatc cgtcaacacc gcagccaatc aggatattca
      361 ggttaaatgc cgattcccct tcgcggacaa actcacacat atagagattg aggaactcgt
      421 cttcggtgtt ttcatcacga atttcgtcga tatcggtgtg tttccagccg tgattgacca
      481 catcttccag cgtgacaatt tgccgccacg tccggtcagg gcagataagc ccgttatgca
      541 gcgttttcca gtccacagaa aaacgctggc gtttatgcgt ggcctttttc tcgttccagc
      601 ggtcgccgtt ccagtaggcg tatgcctcgt gcgtttcggt ggatggcgta gagaagtagg
      661 tgcgccgcag tccgctgagg gttgccatag cgccagccac cttgcgcagt tcagcaaagc
      721 gactgaccca gaaaaattca tcaaaataaa aattgcccgt ataggactgt gccgacgcag
      781 cagaagtgcc gagaaaatgc agctctgctc cgttggagag gatgatttta tcgccccctt
      841 tcagctccac atcaacttca gccgcggcct tctgaataat gcttttaaac tggaacgcct
      901 gacgacgcga cgcagacaaa aaaatctggt tacgctggta aggttgcgcc acatcgtcac
      961 gcagcgccat cagcagagct tcctgtgcaa aataccaggt cgccccaatc tgtcgggatt
     1021 tcaggatcat cctgttacgt atcccggctt ccctgcaaag ggtcagggag tcaaaccagc
     1081 cccgctgatg ccactccagc ctgctgatga ttttttcccg cagtgcggca atctgttccg
     1141 gcgtgaaatg atttttgagt tttttcgccc ggcctttctt tcctgtgacc gtcgcatccg
     1201 gctggccatc atgcagtttt ttaagctgcc gggtcagcag gtctatttcc ttaaagtcac
     1261 cgcctgtttt attctgtttt tcagtaagct ggatgaggcg cgcatcgatg gactgcgtga
     1321 cacgctgcac gggtggcgtt tcatcccact ggtcgcgttt tttccacgca taaatcgtgt
     1381 tcgggtttat tcccatcaga cgtgatattt ctgcgggcgg ataaccctgc cagtaaagtt
     1441 gccgcgcacg ctggcgcaca aaagcgtcct gaatcattgc tccccctgag taattacagg
     1501 aagattaccc gcgcgcgaaa ctgttctcct taaccccctg ttctggccgt tttcttacaa
     1561 caaaatccct ttgtatcagc ctgttacgct ttgccatcat gactgaagaa ccagtcagag
     1621 gggcaaaaac tatggctaat gaaaaaaaga catcccgcaa aaagtttcgc gtggctgtct
     1681 ccggatcaac tgttgatggc cgtgaaatca gtccggtgca tctgcgtgaa gccgccgaga
     1741 acttcaaccc ggatgtttac gctgcccgcg tgaacgttga gcactatctc tcgccatgcc
     1801 cgtcaagcga attttccgca atgggcgatg tcaccgcgct gagtacggaa gatattacgg
     1861 aaggcccgct ggccggacgc actgcgctgt atgcagaaat cgaaccgacc gagcgcatga
     1921 agcagcttgt cgctgacggc aagaaaatct attccagtat cgaactgcac ccgcagttct
     1981 ccgttaacgg gcgtgcctat ctggtcgggc tggcgatgac cgacaccccg gcaagcctgg
     2041 gcactgagcg cctgaaattc acggcacagc agcgtcaggc ggtgatgacg ttcaacagta
     2101 tccagggtga agcaccgctt atctccgaag ccatcgagtc tgaaatcatc gaaatggcag
     2161 aacaacgcca ggaagaaggc acccagtggt ttaaccgcgt aatagggatt attggtcgtg
     2221 gccgcaaagc ggatgacgcc agtttctccc gtattcagga agcggtggaa ggcgttgcaa
     2281 catcacaggc cgacattatc gaccgtttta atgtgctgga aacccgccat cagcaggacc
     2341 gccagaaaat cacgtcactg accacagagc tgacagcact gaaggaaaaa ctgcgcacgc
     2401 aggacggcga tccgcagaac cgcttcaccg caacgggcgc agcctccgac cagctggctg
     2461 acttctgata agacaaagga gcaaattttt tatgaatctg gtgatgtcag atattacccg
     2521 caacaagctg ggttgctata tggcgcagca ggcgtcgctt aacaatatcc cggtatctgc
     2581 actggtatcg cgatttaccg tggaacccgc ggtgcagcag cgttttgaaa acgcctcaaa
     2641 ggaaagtacc gaatttacga aaagaattaa cgtgatcggc gtgaccgacc agaaaggcga
     2701 aaaaatcctc ctggacacca ccgggccaat tgcgcgcacg aatatcagtt atgacggcat
     2761 caaacgccgt aacccgaata acgtgatcga tatgaagtct cgtcagtacc agtgcgaaca
     2821 ggtgaactac gataccttta tttcgtaccc acagcttgat acctgggcgg cccacagcga
     2881 ttttcagtcc cgtatcagta cacagatcgc ccggcaggta gcgcttgatc gcatcatgat
     2941 tggctttaac ggcacatccc acgcctacga gtctgatttt cacaccaaca agctgcttca
     3001 ggacgttaac gtgggctggc tggagcacat cagaaccgat gccagcgagc gcgtaatgaa
     3061 tgacgtgacg ctgacctccc gcaacatgga caacactgtg gcgcacgcgg gtaagtatgc
     3121 gaatgccgat gctctggtac aggatgcgcg ctcatccctg ctggatgaat ggcacaagga
     3181 agctgacgac ctcgtggtga ttatggggcg caacctgttt aactcgctgc gtctgcccgt
     3241 gctgaacagc atcagcggcc agaatcccaa tgcggaatta cttgccgggc agctcatcct
     3301 gtcatcgcgc accattggcg ggctgggcgt gttccttgcg ccgttcttcc cggatgcaac
     3361 gatgcttatc acctcgttca acaacctgtc gatttactgg cagaaaggtt caatgcgtcg
     3421 cctgatgaaa gacgagccgg aatacaaccg catcgccacc taccagtcca tcaatgacgc
     3481 ttatgtcgtt gaagactatg gcaagtgcgc gatggtcact ggcctgaagt tcgccgacag
     3541 ctaatcaact cacggcgggc atcatgcccg cctgtaacgg agagaaaaaa tgattactcc
     3601 tgcacaacaa cactggcaga acgtgatggc acagcgcgca ggccgggcga atgaaggtgt
     3661 ggaccacgcc gcgtgtaccg cgcatgaaga ggtgctgtat cgtctgcgtc tggcacaggc
     3721 caggctaaag gccatacagg ccagaagcgc gaaagccgcc atcaaaaaag agttgttgcc
     3781 ggacttttcc ggctggattg agggaacgct ggaggctgac tgcgggcaac aggacgaagt
     3841 aattgccacg ctgatggtgt gggcgattga ctgcggcgat cttccgctgg cgctgcgtat
     3901 tggtgcatat gtggtccgtc acaacctcat catgccggat aactttggcc gtactgctgc
     3961 cacggtactg accgaagaaa tctgcaaccc ggtactgacg caggccggga cggatgccga
     4021 cgcggattta tccgccttta tcgaaccact ggacacactt tgggaaattg tcgccaacca
     4081 ggacatgccg gacgaagtgc gcgccaaatt atgcaaggcg tgtgcctttg cccgtcgtgg
     4141 cctgaccgat gcagacaaca tggcctcatc actgaagctg ctgcgcgaag cgatgcacct
     4201 gaacccgaac gcaggtgtga aacgcgagat tgcaaccctt tcccgcgccc tgaaaaaagc
     4261 cgattccgca gccgaaccag aagacgccag cgcacagcag gcgcaggacg aaagcagcaa
     4321 aagtaaaaag acaacgcgga agcctgcaac acgaaaaacc accgcgacgc agaaggcaaa
     4381 gcgcggttaa cgactgaccc cgtcagcggg cggcgtgcgc ggtgttccgg tttgactccg
     4441 tgaccgttta caccgcgcac ccaccgcccg attttttcag gagtgaaccc catgagtatg
     4501 gttgccagaa ctaaccccgg ccccgcagag gacgacatca ccgataccga tgatggcgac
     4561 actcgcattt cagcgggtgc attctggccg gatattgtgc tgcgtgagct gcgtctggcg
     4621 gtacgactga cgggccgcgt gaccacctcc cgcctgctgc ataccgccac cggggccata
     4681 gcacacgtta cccgcgaact ggaagcatgg cagcaggaac aacaggcggc tggctatcag
     4741 acgctggccg atgttccggc ccctgtaatt aacggagaaa gcgtcaatct ctggcactgg
     4801 cgcaatgctg tttataccgc cacacgcgcc ctgattctgg agcgttaccg cgatgcggac
     4861 acaacggaca agggcgaccg ccgggcggac gcactggata tacagacatc ggatttgtgg
     4921 cgcgatgtga gctgggccat ctctgacatt ctgggacgac cgcgaatgtt tgcggagctg
     4981 tgctgatgaa agtgaaggca ctggaaggcg acaccgtgga ttcgctctgt ttccggtact
     5041 acggcacgac acagggcgtc accgaaaagg tgctggatgc caaccccgga ctctgtcagc
     5101 aggtatttct ggacgccggg caggaagtgg agatgccgga gccggagaag aagaaacgag
     5161 aaatgattca gttgtggggg gagtagcagt gagcaccatt caaacaggga tcacagagca
     5221 ggttattgcg tggctctttg accacctgcc aacggtgtat gcagtaggcg cggctgtcag
     5281 catttccgcg ctgatgagtc tttatgacgg acgaacactg gttcagaccg taacgggatc
     5341 gctggcgtgc ggcgttcttg ccatggccgt ggccgggtcg ctgcgcttct tcgggatccc
     5401 tgaggatgca gtgacgtttt ttggtgcctc aatcggtttt atgggcgcag agaaagcacg
     5461 cgacaaggtt attgcaatat ttgatcgcag ggtgaaggag aggaacgaat gagcaacaca
     5521 tttaaattca gcagccgaag cgaaaagaat ttgcagggtg taaatcctga tctggtgaaa
     5581 gtgacccgac gggcgctgga aatctcggaa gtggattttg gtatcaccga aggattgcgc
     5641 agtcgttatc gtcagaagca gctcgtgacc acaggcaaga gccagaccat gaacagtcgc
     5701 catctcacag ggcatgccgt ggatgttgtg gcttatgttg gcagccaggt gtcatgggaa
     5761 tggccgctgt acgaaaaaat cgcagcagca ttcagacagg ccagccggga actgaatatt
     5821 ccggtggaat ggggcggcga ctggaagacc ctgaaagacg gaccgcattt tcagttacca
     5881 cacggagcct atccggcatg aagctctggc ccacgcttgg cgtcgctttc cttctgattg
     5941 ccgcatgggg aacatccatg cgtctgtcgt ggtcgctggg ccgggagaac gccagaaacg
     6001 aagcgcaggc cagcaccctg aaaagtaccg tcgacacact gaatatcatc agcgccgggg
     6061 tacaggatat gcagcaggtg ctggctcaac tccgcgcgga caatcagcaa cgcaatcagg
     6121 acggagaggt aagacgtgaa cagctacgca acgatattgc aaaagatgaa tgcgcccacg
     6181 ctttgcctga cgctcgtttt actgacaggt tgcgcaggca cgcagaacgc gccacggcca
     6241 gcgccgtcag tccggcttat accgcagacg ctgaccatgc cggtaacgcc tccccccttc
     6301 cctgaccaac ccacatgggg aaacctcgga atatggggcg accgccttct ggatgcactg
     6361 gaaacctgta acgcggataa acgggccatt gctgaactgg ataagagaat agccgaactg
     6421 acacaccaga cgggagtaac acaatgacca gtaagaactt tgcactgatt acagccatga
     6481 cacaggctga actgactcaa aaggtgaatg aacatcttgc gaaagggtgg catcttcagg
     6541 gggagacgcg ggttgcctac gaacccggca ccccgtggta tctaatgcag gcaatggtgg
     6601 ccgatggcac tacagacatc tcacctgatt ccccccagca cggcagcgtg ccggagtggt
     6661 attacgtggt ggtacttgct ggtcaatcca atgccatgtc atatggtgag ggaatgccgc
     6721 tgccggattc ttacgatgcg ccccacccac gcattaagca actggcccgt cgcaacacag
     6781 tgactcccgg tggtaaagca tgcgcattta acgacatcat tccggcagat cactgcctgc
     6841 atgatgttca ggatatgagc gcactgaatc acccgaatgc agacctgagc aaagggcagt
     6901 atggctgtgt cggacagggc ttgcatattg ccaaacgcct gttgccttac attccacaga
     6961 atgccgggat tttactggtt ccatgctgtc gtggtggttc ggcattcacc cagggcgcgg
     7021 aggggacatt cagcgagtcc acaggagcca gtcaggattc ggctcgctgg ggtgtgggta
     7081 aacctttata tcaggacctg attttgcgca cgaaggccgc attgcagaaa aacccaaaaa
     7141 acatgctgct ggccgtatgc tggatgcagg gcgaatttga catgagcgcc gctacgtact
     7201 cacagcaacc tccgctgttt gcggccatgc tgaaacagtt tcgtgcggac attaccgagt
     7261 ttaacacgca gtgtcatgga ggcagagcgg caagtgtgcc atggatttgt ggtgacacga
     7321 cgtattactg gaaaaacacc tacggcacgc agtacgacac catttacggg gcgtacaaaa
     7381 acagggagag cgacaacgtt ttctttgtgc cattcctgac cgatggtagt ggcaacaata
     7441 cctccaccaa cgcaccaacg gaagatccgg atgctgcaag tgagggatat tacggttcgg
     7501 catcccgaac gaacaaaaac tgggtatcat caaatcgcca gacgcatttc agttcatggg
     7561 cgcgcaggag cattattccg gatcgtatgg caaccgctat tctgaacgta gccggtcgca
     7621 ccttagcctt catcagtggt aaggcaccgg aaatcaaacc ctcgcccggc ggcgacacgc
     7681 catcggggcc gtctgaagat gcatccgtac gcacaatctc cctgttgccg acagccggag
     7741 acgctgctgc gcagggctgg agcattaaga atggcggaat tcagttgtca gagggtgtat
     7801 ttaagatcac caagcagagc aataaagcct ggtccctgac gcgcccggtg gatgacgcag
     7861 tctccctgct gacacggggt ggcagactga gctgtaagtt tcgactgtca ggcgcactga
     7921 ccaacaatca gttcggtctg ggaatttatc tgtataccga tgtagcgtta cctgacgtcg
     7981 tggcgatgac cgggactggt aacccgttcc tgatgtcgtt cttcacccag accacagacg
     8041 gcaaactgaa tctgatgcat cacaagaaag caggaaacac aaagttgggc gagttcggga
     8101 attacagtaa cgactggcag acgctggagc tggtgttcac cgccagcagt gccacggtta
     8161 ctccgaaact gaatggagtg gctggcccgg cattccaggt cataaaagac agtctgacac
     8221 tggggctgaa tgcactgacg ctgacggata ttaccaaaaa tgcaacgtat ggcgttgaga
     8281 tagaaagtct ggtgctggaa ataaatagtc ctgcagcgta aggaagaaca ggagagcaaa
     8341 acagatgctt aagacaaaca gcctccggaa gggcatgatt catgggtgtc gctggtgtca
     8401 ggccaacccg gaaaaattca ccattttcgt ggagagcggc aacattgaaa cgaccggaga
     8461 aacgccctcg tttgtttacc gctatcagat ggtgatgttt gtcatggatt acgccgggga
     8521 gctggacgac ctcacgctgc cgctactggc gtggttatcc gaaaatcagc cgcaattgtt
     8581 gcttaatccg gagcgtaatc aggacatcaa attctccgcc gttatcaatg acgatgacag
     8641 cgccgatctc ctgtttacgc tcactctgcg ggaacgcgtt cgcatcacgc gcagcagtca
     8701 gggggcaccg caggcagaac acctgcagga gccaaaaccc cgtctgccat cttccgaagg
     8761 cgactggtcg catgtattcc aggatgtgac gtggggtgaa agcgatggat aaggcattca
     8821 cccgcgtgga tgaaaccttt gaggctatcc gcgacagcct gaatcagcag gccatcaata
     8881 acatcgccag aaagctggca caggatttac gccgcgccca gcaggcgcgt atccggtcac
     8941 agaaagcgcc ggacgggacc gcgtggacac cacgcagacg ccgcgtaacc cggatacagg
     9001 agcgcattcg ctttatctgg aataacgaag cacgcacgct gaaaaactgg catcacgaca
     9061 cggggaaata cgggcgaacc attaccgggt gggatgagga taaaaacaat atccgcacgt
     9121 tttaccggga tgacatcgac cgctttctgg aaatacgcac ccggcgcatc aaccaggaca
     9181 gcacaaagcg cgtccccatg ttcgtaaaac tgcgcaccgc ccgctacctg aaagcccgtg
     9241 cagatgcttc cggtgtgacg gtgggttaca gcggcgtggc cgcacgtatt gcccgcgttc
     9301 atcagttcgg tgagcgcgat caggttgcgc cgggcatttt caccgattac ccggtacgtg
     9361 agctgttggg catcagtcag gcagatgaac gcctgattta taacacggtg ctgggccgga
     9421 ttgcggaggc tgtacggtga gcgcagaact catgcgactg ctgagcaaca tcatccgcac
     9481 tgggatcatc tctgaagttg atgagaagtc ctggtgcgtg cgcgttcgca gcggcgaact
     9541 ggaaacaggc tggttgcgct ggaacaccac gcgcgcggga gccttcaatg tgtggctgcc
     9601 gccatcaccc ggcgaacagg tggtaattgc ctgcattggc ggcaacccgg aaaccgccat
     9661 gataattggc agcctgtgga gtgatgccaa tccggccccc ggcaaaagcc tgaaagaaat
     9721 cgtggtcagc gcgccggatg gcgcggtgtt ccgctacgac gcggacgcag gcgcactgag
     9781 cgccagcggc atgaaaacgg ccaccctgca ggcatccgtc agtgtgacac tggacacgcc
     9841 cgtcgtggaa tgcacagacc ttctgagaac ggcgacgctt gacgtcacaa aagggggaaa
     9901 gatgagcggc aatatcacgc acagcggcgg caatttcacc tcaaacggca tcacagtgca
     9961 tacgcataaa cacggtggcg ttaaaggtgg cagcgattcg acaggaggcc cgcagtgaca
    10021 acccgctaca caggaatgaa cccggacgga acgggaaacc tgaacgatat ggagcatctg
    10081 aaacagtcag tcagggacat cctgaccacc ccgctggcaa gccgggttat gcgacgggaa
    10141 tatggcagcc ttgtgcccga tttaattgac gaacccatga ataacaccac tcgtctgcaa
    10201 tgcatgagtg ctgccgtgat tgcgctgaca cgatgggaac cccgcattgc cctggacgcc
    10261 atcgacgttg tctggaaggc aggaggccgc gccggggtga cgctgtcggg cactgtcatg
    10321 cagaccatgc agaatgttga attaaccatc acgctgaggg agtaaatcat gcctgccgtt
    10381 gacctttccc agttaccgga acccgccatc atcgcggagc ctgactttga ggcaattctg
    10441 gctgacacaa aggccatgat gattgcgtcc tatcctgccg aacagcgtga agccgtctcc
    10501 gccgcgctgg agctggaatc ggaacccctg aacgttatcg cccagacaac agcgtttcgt
    10561 gaaatgctgt tacgccagcg ggtcaatgag ggggcacgcg cctgcatgtt aagccacagc
    10621 gccgggacag acctggacaa cctcgcgggc aatatgaaca caaagcgcct gaccatcact
    10681 ccggcaacgg ataccgccga cgcagtgatg gaaagtgaca cctcgctgag actgcgggcg
    10741 cagcgggcgt acgatggcct gagtgttgct ggcccgtcag gtgcatacga gtattttgcc
    10801 cgcagcgcca gcggtctggt gcgtgatgcg cgggctatca gtccgtctcc ggcaaatgtg
    10861 acggtttcca tcctgtccac tgaaggcgac ggcacagcaa cggaggcgtt gcttaatacc
    10921 gttcgcgccg ttctgaatgc agaggatacc cgcccggtgg ccgaccgcct tactgtacag
    10981 agcgccagaa tcgtgacatg gcggctgaat gcaaaactgt acttttaccc cggcccggaa
    11041 tccgaaccta ttctggcggc ggctgaatcg tcgttcagga agtggctggc tgagcagggg
    11101 cttatcggtc aggacgtggc gttgtccgcc attgctgccg cactgcatgt gcacggtgtg
    11161 caacgcgtgg agataatcga acccacacag aatatggcca tcagcgacat acaggcggcg
    11221 cgctgtgagt cgttcaccat cagcgaaggt gggcgcaatg agtaattcac tgttaccacc
    11281 atcagccagc agtttcatgc gttgtgccga agctgtcgga acgcgcatta cagacatccc
    11341 ggtagacctc aacacgctgt ggtcgccgga cacctgcccg gtgcacctgc tgccttatct
    11401 cgcctgggca ttttccgttg accgctggga tcgcaactgg ccggaagaga caaaacgaca
    11461 ggtgattcgt gatgcatggc tgatacaccg acacaaaggg accatcagcg cactgcgcag
    11521 ggccattgag ccgctgggat acctcattcg cgtgtctgag tggtgggagt tcggcggaga
    11581 accgggaaca tttaccgttg aagtcggcac actggacagt ggcgtgacgg aggaaatgta
    11641 tctggaaatg gagcggttga ttgctgatgc ccgtccggtc agccgccaca tgacagggct
    11701 gaatatcatt caggaaattc cgggggatat ttttgcagcg gcggcaactt atgacggtga
    11761 agttattacc atttatccgg acgattaagc atgagtacca caacacgaaa atttaaaacc
    11821 gttatcaccg atacaggtgc ccaaaaatta gctcaggcag ccgcgccaga tggtaagcct
    11881 gtccgcctga ctcatatggc cgtgggcgac ggtggcggcg cgttgcccac accagacagt
    11941 aagcagaccc gtctggtgca tgaggtgtgg cgacacactg ttaatcgcgt catcctggac
    12001 gcaacacatc agaaccgcat tattgcggag ctggttattc ctccagaaac gggcggattc
    12061 tggatccggg aaattggtgt gtttgatgag cacggcgatt taatcgcggt gggcaatact
    12121 gccgaaagtt acaagccagc cgttgccgaa gggtccggac gtgcacaaac atttcgcacc
    12181 attctgaccg tatccagcac tgccactgtg gcgcttaccg tggataacac catggtgatg
    12241 gccacagtgg attacgtgga taacaaactg aaagagcatg aacagtcacg acgtcacccg
    12301 gatgcctcgc tgaccgcaaa aggctttgtt caactcagta gcgccactaa cagcgattct
    12361 gaaacgctgg ctgcaacgcc gaaagcggtt aaggtcgcgt atgatcttgc taacggaaaa
    12421 tatacagcgc aggatgccac aacagcgcga aaaggccttg tccagcttag tagtgcaacc
    12481 aacagtacat ctgaaacgct ggcggcaaca tcaaatgcag taaaagctgc ctatgacaat
    12541 gctgaaaaac gtctgcagaa aactaagaat ggtgaggata tctctgataa agacaccttt
    12601 acgaaaaata tcggtgcctg ccgtgcatat agtgcagagc tgaatattgg tggagatagt
    12661 gaagcatgga caactgcgca gttgattttt tggctagaga gtcagggggc atttaaccat
    12721 ccttactgga tgtgcaaagg ctcatgggct tatgcaagta ataaggtcat tacagacaca
    12781 ggttgcggaa atatttgtct tgcaggtgct gttgtggaag ttattggcac ccgcggcgca
    12841 atgaccatac gcattaccac accgagtaca tccagcggtg aaggcatccc taatgctcaa
    12901 tttacttata ttaatcatgg tgatgcttat gctcctggct ggcgaaggga ctataactcc
    12961 agaaataagc caacggcatc agagatcggg gcgttaccgt caggtggaac agcagtatca
    13021 tcagttaatc tgtcttcaaa aggtcgggta accgcgctga cagacaatac gcagggggca
    13081 acaggtcttg agttatacga ggtgtataac aacggatatc caacagcgta tggaaatatc
    13141 attcacctga aagggatgac agccgttggc gaaggtgagt tactcatcgg ctggagtggt
    13201 acaagcggtg ctcatgctcc ggcatttatt cgttcacgac gggatacgac cgacgcaaac
    13261 tggtcgccgt gggcgcagct ttacacctcg gctcatcctc ctgcagagtt ttatccagtc
    13321 ggtgcaccaa tcccgtggcc atcagatacc gttccgtctg gctatgccct gatgcagggg
    13381 cagacttttg acaaatctgc atacccgaaa cttgcagcgg cttatccgtc aggcgtgatc
    13441 cctgatatgc gtggctggac gattaagggc aaacctacca gtggtcgggc cgtattgtct
    13501 caggaacagg acggcattaa atcgcatacc cacagcgcca gcgcatccag tacggatttg
    13561 gggacgaaaa ccacatcgtc gtttgattac ggcactaaaa cgaccagttc atttgattac
    13621 ggcacaaaaa ctacgaatag cgctggaaat cattcacaca atatacctgt tggtcacact
    13681 ggcgcgggga atggtgtatc agccggtttt aacgctgcgt taggtactgg taccacgtcg
    13741 agcgcaggcg agcatgctca caatgtatat atcggtgccc ataaccacac tatcggcatt
    13801 ggtgctcatg cccattctgt cattattggt ccccacggac acaccatcac cgttaacgct
    13861 acgggtaacg aagaaaacac cgtaaaaaac atcgcattta actatattgt gaggcttgca
    13921 taatgacatt cagaatgagt gaacactcac ggaccataaa aatttataat ctactgaccg
    13981 gaaccaatga gtttattggt gaaggtgatg catacattcc acctcataca ggtctgcctg
    14041 caaacagtac cgatattgcc ccgccagata ttccggctgg cttcgtggcc gttttcaaca
    14101 gtgatgaggc atcgtggcat ctcgttgaag accatcgggg taaaacggtt tatgacgtgg
    14161 catcagggga ctcgttattt atttctgaac tcggtccatt accggaaaat gttacctggt
    14221 tgtcgccgta tggagagtat cagaagtgga acggcacatc ctgggtgaaa gatgcagaag
    14281 cagaaaaact gtttcggata agggaggcgg aagaaacaaa aaacagcctg atgcaggtag
    14341 ccagcgagca tattgcgcca cttcaggatg ccgtagattt ggatattgca acggaggaag
    14401 aggcatcgtt actggctgca tggaagacat accgggtatt gttgaatcgt gttaatacag
    14461 cggtagcagc ggatgttgag tggccagtcg ccccacaata aaaagaaaaa gccatcgata
    14521 gaaatatcga tggctttatg tactctattt atacaataca gcaccgctct ttttagttat
    14581 atatgtgcag ttcgatggta tatctttatt tataaaagac attgcaccta tttttacatt
    14641 atccccaatt ttacgggata atccaatgat gcaacaatta gctccgatat caacgttgct
    14701 accaattttt actcttgaac caggcatgtc accatctatc tgtccaatgg tagtattctg
    14761 tcgtaacacc agattttcac cagcatcaac
//
LOCUS       DAZGLJ010000114        14627 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000114 DAZGLJ010000000
VERSION     DAZGLJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.114"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..85)
                     /locus_tag="ACHK49_002985"
     CDS             complement(<1..85)
                     /locus_tag="ACHK49_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFO9263600.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMA"
     gene            complement(85..1293)
                     /gene="ybjJ"
                     /locus_tag="ACHK49_002986"
     CDS             complement(85..1293)
                     /gene="ybjJ"
                     /locus_tag="ACHK49_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9263601.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            1377..1913
                     /gene="rcdA"
                     /locus_tag="ACHK49_002987"
     CDS             1377..1913
                     /gene="rcdA"
                     /locus_tag="ACHK49_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO9263602.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(2088..3773)
                     /gene="ybjL"
                     /locus_tag="ACHK49_002988"
     CDS             complement(2088..3773)
                     /gene="ybjL"
                     /locus_tag="ACHK49_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO9263603.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            4043..4420
                     /gene="ybjM"
                     /locus_tag="ACHK49_002989"
     CDS             4043..4420
                     /gene="ybjM"
                     /locus_tag="ACHK49_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO9263604.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(4450..4707)
                     /gene="grxA"
                     /locus_tag="ACHK49_002990"
     CDS             complement(4450..4707)
                     /gene="grxA"
                     /locus_tag="ACHK49_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO9263605.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            4867..5154
                     /gene="ybjC"
                     /locus_tag="ACHK49_002991"
     CDS             4867..5154
                     /gene="ybjC"
                     /locus_tag="ACHK49_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO9263606.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            5138..5860
                     /gene="nfsA"
                     /locus_tag="ACHK49_002992"
     CDS             5138..5860
                     /gene="nfsA"
                     /locus_tag="ACHK49_002992"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO9263607.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            5921..6823
                     /gene="rimK"
                     /locus_tag="ACHK49_002993"
     CDS             5921..6823
                     /gene="rimK"
                     /locus_tag="ACHK49_002993"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO9263608.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            6911..7387
                     /gene="ybjN"
                     /locus_tag="ACHK49_002994"
     CDS             6911..7387
                     /gene="ybjN"
                     /locus_tag="ACHK49_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO9263609.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            7738..8850
                     /gene="potF"
                     /locus_tag="ACHK49_002995"
     CDS             7738..8850
                     /gene="potF"
                     /locus_tag="ACHK49_002995"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO9263610.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHVYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDNVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            8945..10078
                     /gene="potG"
                     /locus_tag="ACHK49_002996"
     CDS             8945..10078
                     /gene="potG"
                     /locus_tag="ACHK49_002996"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO9263611.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            10088..11041
                     /gene="potH"
                     /locus_tag="ACHK49_002997"
     CDS             10088..11041
                     /gene="potH"
                     /locus_tag="ACHK49_002997"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO9263612.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            11038..11883
                     /gene="potI"
                     /locus_tag="ACHK49_002998"
     CDS             11038..11883
                     /gene="potI"
                     /locus_tag="ACHK49_002998"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO9263613.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            11943..12431
                     /gene="ybjO"
                     /locus_tag="ACHK49_002999"
     CDS             11943..12431
                     /gene="ybjO"
                     /locus_tag="ACHK49_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO9263614.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            12472..13599
                     /gene="rlmC"
                     /locus_tag="ACHK49_003000"
     CDS             12472..13599
                     /gene="rlmC"
                     /locus_tag="ACHK49_003000"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO9263615.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(13774..14505)
                     /gene="artJ"
                     /locus_tag="ACHK49_003001"
     CDS             complement(13774..14505)
                     /gene="artJ"
                     /locus_tag="ACHK49_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO9263616.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
BASE COUNT         3540 a         3618 c         3841 g         3628 t
ORIGIN      
        1 gagccataaa ccgctcacgg ttataggttt tttgatcgct taagaaagta ccatccatgt
       61 ctaccgcaat taatttaatg ctcatcaact attctccatc gccgtctgcg ttttggtatc
      121 gggtttggcg acggctttcg cgacaatagc cgcgagaata accagcgcca gtacaaccag
      181 cattgcacta cgtaatccat aatgttcgcc gagatagccc agcagcggcg gcccgacgag
      241 gaaagccaga taaccggtcg ttgctaccac actgacgcgg gttggtgcat cggggccggt
      301 atcgctggcg gcagaaatgg tcagcgggaa gcccagcgag gcacccagtc cccagagtac
      361 aacagacacc ccagcgaccc aggcgctatc gacaaaaata atcagcccaa tacccaacgc
      421 tcccattagt gcactggccc gaaccacggc aacgcgactg taacggtcga tgaaccaacc
      481 gccggtaaag cgtccaacag tcatccccag ggtaaaaccg gcataaatca gcgagccgga
      541 agtagggcta aaaccgtgac cgtcaaccat taataagggt aaccagtcgt tggcagaacc
      601 ttcggcaaag gccatcgcca gcaccacaac accaatcagc agcaactgga tatcgcgata
      661 aaaaggtacg cctttttcac catgctgggt gccatcggca gcatttttgc ccgtaccgtc
      721 agggattgcc tgaatggcga tataaatagg cgcgattcct accagcgccg ccaataaaat
      781 gtgcaccgtt gccggaacgc caaaggccgt cagtgccatc ccgacaccag cgcctgccag
      841 cgtgcccagg ctataaaaac cgtgcatcat cggcaaaacc gttttattca tttctcgctc
      901 aacggcggca ccttcgacgt ttatcgccac ttccgcagaa ccaaaacttg ccccaaagac
      961 gccgaggcca accgcgaaga gcaggggtga tgtcagccag agtgccagac ttaatatcat
     1021 catcccgatc aatgcgcagg acatcgtgac caggatgaca ttacgtgtcc caaagcgttt
     1081 cactaaccac gccgagcaga gaataccgct catcgaaccg atcgacagac caaagagaac
     1141 accgcccatt tcagcgatcg agacagagag aatatcgcgg atagcaggcg tacgggttgc
     1201 ccaggacgcc attaacaggc ccggcaaaaa gaagaacata aacagcgccc aggttcggcg
     1261 tttcaatgca ttacgtgaag aatttacggt catagatcac gtcaaaataa gaagagggaa
     1321 gacaacatta gcaaggttgt gtacatttgt acacaattgc agacagagga aatgacatgc
     1381 gtcgcgctaa cgatccgcaa cggcgagaaa aaattatcca ggccacgctg gaggcggtga
     1441 aactttacgg aatacatgct gttacgcacc gcaaaattgc tacccttgcc ggggtaccgt
     1501 tgggatcgat gacctactat ttttcaggaa ttgatgagtt gttgctggag gcgttcagca
     1561 gttttactga gattatgtcc cggcaatatc aggcattttt tagcgatgtt agtgatgctc
     1621 aaggcgcatg ccaggctatc accgatatga tctacagctc acaggttgca acgccggata
     1681 acatggagct gatgtaccag ctctacgcgc tggctagccg aaaaccgcta ttaaaaacgg
     1741 taatgcaaaa ctggatgcag cgcagtcagc aaacgctcga acaatggttt gaacccggaa
     1801 ccgcccgcgc gcttgatgcg tttattgagg ggatgacgct gcattttgtc accgaccgta
     1861 agccgctatc gcgcgaggag attttgagga tggttgagag ggttgcaggg tagtagataa
     1921 attttagata acaaaaaacc catcaacctt gaaccgaaat ggcggggttg atgggctcca
     1981 caaaatgggg acatcaaaga aaagcagtgg cactaattaa gactgatgcc ctgcggaaaa
     2041 gttctgcggt tgtgcaaaaa aatttcattt tcagggcaac ttcagtttta tcctaatcct
     2101 ggccatacca tgacgatgat tgtccctgcc agcgtcagca ggacgttggc gattgcgtag
     2161 gtgcccgcat agcccagcgc agggatgtta ctgcgagctg tatcactgat gatctccatt
     2221 gccggcgcgc aggtgcgtgc gcccatcatt gcgccgaaca acagtgcgcg gttcattcgc
     2281 aatacataag caccgaacaa gaaacagata accacgggca caagactgac aattaatccg
     2341 gcaatcaaca tctgaccgcc aatcgcgccc aggccgttat taataccgct accggcgctc
     2401 agaccaacgc ctgccataaa caccatcaag ccgaactctt tcaccatgct taatgcacct
     2461 tgcggaatgt aaccgaaggt cgggtggtta gcacgcataa agcccagcat aattccggcg
     2521 aataacaacc cggcagcgtt ccccatgccg aaactgaatg tgctgaactg gaaggtgatc
     2581 atcccgatca tcagcccaat aacaaagaag gcgcaaaatg ccagcaggtc agtgacctgg
     2641 ctgtgaatcg agataaagcc gatgcgatcg gcgatggttt ttacgcgacg ggcatcaccg
     2701 ctgacttgta aaacgtcacc tttgttaagc acgacgttgt catctatcgg catctcaatc
     2761 tggctacgaa tgacgcggtt aaggaagcaa ccgtgatcgg tcaacttcag ttgtgcgaga
     2821 cgtttaccta cagcgttatg gtttttaacg accacttctt cagtgacgat acgcatgtcg
     2881 agaaggtcac gatcgaaaac ttctttaccg ttacggaagc tgggatcgag tcgggcatgg
     2941 gcgtcgggat agcctaccaa cgctatttca tcgcccattt gtagcacggc atcaccgtct
     3001 ggatttgcca gaatcccgtt acgtcgaata cgttcaatgt agcagccggt ttgtcgataa
     3061 atacccagtt cacgcagatt tttgccgtcg gtccaggcca ccagctccgg gccgacgcga
     3121 taggcgcgga tcaccggtaa ataaacctta cggttggcat cagtgtccag gccacgttcg
     3181 cgggcgattt gctgggcgct ggtctgtaag tcctgatgct gcaatttcgg caagtaacgc
     3241 gcaccaacaa tcaaactcac cagaccgatt aaataggtta aggcataccc gaggctcaga
     3301 ttatccagtg ccagtgagag ctgcctgctt tccatgccgg aatgacgcag tgtatcgcca
     3361 gcaccgacca gaaccggtgt cgacgtcata gagcctgcta acataccggc cgtcaggcca
     3421 atatcccagc caaacagctt acctaaccct aaggcgatca ccagcgcact gccaaccatc
     3481 accagtgcta acattaggta atttttccca tcgcgaaaaa aaatggaaaa aaagttcggt
     3541 ccggcttcga ccccgacgca gaaaataaac agcataaagc caagattaag cgcatcggtg
     3601 ttaatgctga aatgttgttg gcctaataac agcgatacga ctaaaacgcc aatggaatta
     3661 cccagttgga tcgaaccaag tcgtaacttt ccgagacata gcccaagcgc gaggaccaca
     3721 aataataaca gaatgtaatt cccatttaac aattcggcga cgtttatatt cacggaggct
     3781 aacttcttgt ttactagtaa gctgttgaaa gaaatggtaa tttacgataa tgttttttac
     3841 cagaattcag ggcgcagatt cattcagcgc acctaaacga tagtaaagta acaatatatt
     3901 ttactagtgt aatcacatta ggtatcaacg gctatatgaa ttgcgttggc ctatattagc
     3961 atggaatgcg aagcggcttt atcttactga acgccacact ggcgaaaaat gtgttcgata
     4021 gacgcagtgt caggaggaac gagtgaaaca taaacaacgt tgggcggggg caatctgctg
     4081 ttttgtcctc ttcattgtgg tgtgcctttt tctggcgacg cacatgaaag gcgcttttcg
     4141 ggctgccggg catcctgaaa tcggcttgct atttttcatt cttcctggag cagtcgccag
     4201 cttcttttca cagcgtagag aagtcctgaa acctctattt ggcgcaatgc tggcggcacc
     4261 ctgttcaatg ctcattatgc ggctgttttt ttcaccgacg cgctcattct ggcaagagct
     4321 ggcatggtta ctaagcgcgg tgttctggtg tgcgctgggg gcactgtgtt tcttatttat
     4381 cagtagtttg tttaaaccac agcacagaaa aaatcagtaa agccctcaac gcgagggctt
     4441 gtcagacgat caggcgtcca gattttcttt cacccatgca gcaaaatcgg tatagccgcc
     4501 gatatgttgc tgatcgacaa aaatctgcgg cacggtttct acgggtttac ctgccttttg
     4561 ttgtagatct tctttagtga tcccttccgc acgaatatct acatactgat actgaaaatc
     4621 atcgcgttca ttgctcaatt tctcagccag atcttttgca cgcacacagt aagggcaacc
     4681 cgaacgacca aaaataacgg tttgcattat ttctctcctc atagatttat gcctgtaatg
     4741 atcacgctaa aatgtattcg ctgaaagtag gtttaacctg ttgcattaat tgctaaaagc
     4801 tataactgtt aaacacaata cagtgaaaag ttttagactg aaggctcact ttgcagaggg
     4861 aagcgtatgc gcgcgatcgg taaattgcct aaaggcgtgt tgatactgga atttatcgga
     4921 atgatgctac tggcggtggc gctgctgtcg gtaagcgact ccctgtcgct gcctgagcca
     4981 ttttctcggc cagaagtgca gattctgatg atttttctcg gtgttttgct catgcttccc
     5041 gctgcggtgg tggttattct tcaggtggca aaacgtcttg ccccacagct gatgaaccgt
     5101 ccaccgcaat attcacgttc agaaagagaa aaagataatg acgccaacca ttgaacttat
     5161 ttgtggccat cgctccattc gccatttcac tgatgaaccc atttccgaag cgcagcgtga
     5221 ggcgattatt aacagcgccc gtgcgacgtc cagttccagt tttttgcagt gcagtagcat
     5281 tattcgcatt accgacaaag cgttacgtga agaactggtg acgctgaccg gcgggcaaaa
     5341 acacgtagcg caagcggcgg agttctgggt gttctgtgcc gactttaacc gccatttaca
     5401 gatctgtccg gatgctcagc tcggcctggc ggaacaactg ttgctcggtg tcgttgatac
     5461 ggcaatgatg gcgcagaatg cattaatcgc agcggaatcg ctgggattgg gcggggtata
     5521 tatcggcggc ctgcgcaata atattgaagc ggtgacgaaa ctgcttaaat taccgcagca
     5581 tgttctgccg ctgtttgggc tgtgccttgg ctggcctgcg gataatccgg atcttaagcc
     5641 gcgtttaccg gcctccattt tggtgcatga aaacagctat caaccgctgg ataaaggcgc
     5701 actggcgcag tatgacgagc aactggcgga atattacctc acccgtggca gcaataatcg
     5761 ccgggatacc tggagcgatc atatccgccg aacaatcatt aaagaaagcc gcccatttat
     5821 tctggattat ttgcacaaac agggttgggc gacgcgctaa aaccgccacg tcgatgtatg
     5881 atacgcgggc ttttgaccag gtctgacaga gaggtgcagg gtgaaaattg ccatattgtc
     5941 ccgggatgga acgctctatt cgtgtaagcg gctgcgtgaa gccgctatac agcgcggtca
     6001 cctggttgaa attcttgatc cgctttcttg ctacatgaac ataaatcctg cggcgtcttc
     6061 tattcactac aaaggccgca agttacccca ttttgacgca gtgatcccgc gtattggcac
     6121 cgccattacc ttttatggga cggcggcact gcgccagttc gagatgctgg ggagctatcc
     6181 gctcaatgag tcggtcgcca ttgcccgggc gcgtgacaaa ttgcgttcca tgcaactgct
     6241 ggcgcgtcag ggcatcgacc tgcctgtcac gggcattgcg cattcgccgg atgataccag
     6301 cgatttaatc gacatggtcg gtggtgcgcc gctggtggtc aagttggttg aaggcacgca
     6361 gggaattggc gtcgtgctgg cggagacgcg tcaggcggcg gaaagcgtga ttgacgcttt
     6421 ccgcggtctg aacgcgcata ttctggtgca ggaatatatc aaagaggcgc aagggtgcga
     6481 tatccgctgt ctggttgttg gcgatgaagt ggtcgctgcg attgaacggc gggcgaaaga
     6541 gggcgatttt cgttccaatt tgcatcgtgg cggcgcggca agcgtcgcca gtatcacacc
     6601 acaggagcgt gaaatcgcga taaaagccgc gcgaacgatg gcgctggacg ttgctggtgt
     6661 ggatattctg cgtgctaatc gcgggccgtt ggtgatggag gtgaatgcgt cgccggggct
     6721 ggaaggaata gaaaaaacca ccggtatcga catcgcgggt aaaatgatcc gctggatcga
     6781 acgccacgct acgacagaat attgcctgaa aacgggtggt tagtcgcaat cacattactg
     6841 atcatggttt tgcctgcgct ttttgcgtaa gctgtgccgg tctttttatc gaaagaggtt
     6901 gtacaaaatt atgacatcgc tggtcgttcc tggtctggat acgctgcgtc aatggctcga
     6961 tgacctgggg atgagttttt ttgaatgtga taactgtcag gctctgcatc tgccccatat
     7021 gcagaatttc gacggtgtct ttgatgccaa aatcgatctg atcgataaca cgatcctgtt
     7081 ttctgccatg gcggaagtcc gaccttcagc cgtattgccg ctggcggcgg atttatctgc
     7141 catcaatgcc agttcgctga ccgtgaaagc atttcttgat atgcaggatg ataatctgcc
     7201 aaagctggtg gtttgccagt ctttatccgt tatgcagggc gtaacctatg agcagtttgc
     7261 atggttcgtg cgtcagagcg aagagcagat ttcgatggtc attcttgaag ctaatgccca
     7321 tcaactgctg ttaccgactg acgatgaagg gcaaaacaac gttaccgaaa actatttcct
     7381 ccactgataa ctcctttcga gcacgcagtc gctggtgcag tggctgcgcg ctgcaaaatt
     7441 atctgctgtt tttaaccttt tcttaaagat tatttcactt ctcttgtgtc gatttggctt
     7501 tatcacatag agcaaatacg cataaaaatt tgttaaatac cgttttttaa tccgagctat
     7561 agtctcaaac cctggctaaa gttattcttg cgatgctttt atatagcgag cagtgctggc
     7621 cgggagagag ttctcttttc ttacaccccg ccgataaaaa atatgcacgt ttattgcata
     7681 tcttttagtg tgacaacttt tgttcgtttg ttaacgaact ttcagaagga aagagatatg
     7741 accgccttaa ataaaaaatg gctttcgggt ctggctgcgg gtgctctgat ggccgtctct
     7801 gtcggcacgc tcgcggctga acaaaaaaca ctccacgttt ataactggtc tgattatatc
     7861 gccccggaca cggtggccaa ttttgaaaaa gaaaccggta ttaaagtcgt ctacgatgtt
     7921 ttcgactcta acgaagtact ggaaggcaaa ttaatggcgg gtagcaccgg ctttgacttg
     7981 gtggttccat ctgccagctt tcttgaacgc cagttgactg cgggagtttt ccagccgctg
     8041 gacaaaagca aattgccgga gtggaagaat ctcgacccgg agctgctgaa gctggtcgcc
     8101 aaacacgatc ccgacaataa atttgctatg ccctatatgt gggcgacgac cgggattggc
     8161 tataacgttg ataaagttaa agctgtgctg ggcgaaaacg cgcccgtcga tagctgggac
     8221 ttgatcctca aacctgaaaa tctggaaaaa ctgaaaagct gcggtgtctc tttcctggat
     8281 gcgccagaag aagtttttgc taccgtgttg aattatctcg gcaaagatcc caacagcact
     8341 aaagcggatg attacaccgg accggcaacc gatctgctgt taaagctgcg cccgaacatt
     8401 cgttatttcc attcatctca atacattaac gacctggcaa acggcgatat ttgcgtcgct
     8461 atcggctggg caggtgatgt ctggcaggcg tcaaaccgcg cgaaggaagc gaagaatgga
     8521 gtgaatgtct cgttctcgat tccaaaagaa ggggcgatgg cgttctttga tgtattcgcc
     8581 atgcctgcgg atgccaaaaa caaagacgaa gcctatcagt tcctgaatta cctgctgcgc
     8641 ccggatgtag tagcgcatat ttccgacaat gtgttctatg ccaacgccaa taaagcagcc
     8701 acgccgctgg tgagtgcgga agtccgtgag aacccgggta tttatccgcc tgcggatgtt
     8761 cgtgcgaagc tgttcactct gaaagtgcag gacccgaaaa tcgaccgtgt gcgcacccgc
     8821 gcgtggacca aagtgaagag cggaaaataa tccgcagcct tagatgccgg gcgggcgcac
     8881 cacacccgcc ggcaattcgc accatcatgg tgcgcttgca cacattcaat gccggagagc
     8941 agccgtgaat gacgctatcc ctcgcccgca ggcgaaaacc cgtaaggcgc tgacgccgct
     9001 attagaaatc cgcaacctca ccaaatccta cgatggtcaa catgcggtgg atgatgtcag
     9061 cctgaccatc tacaaaggtg aaatcttcgc gctgctgggc gcatccggct gtggcaagtc
     9121 cacgctgctg cgtatgctgg caggtttcga acaaccttct gccggacaga taatgcttga
     9181 tggcgtcgat ttgtcacagg tgccgcctta cctgcgcccc atcaatatga tgtttcagtc
     9241 ttacgcgctg tttccccata tgactgtgga gcagaacatc gcctttggcc tgaaacagga
     9301 caaactaccg aaagcggaaa ttgccacccg ggtcaacgag atgcttgggc tggtgcacat
     9361 gcaggagttc gccaaacgca aaccgcatca gctttccggt ggtcagcgac agcgtgtggc
     9421 cctggcccga agccttgcca aacgcccgaa actattactg ctcgatgagc cgatgggcgc
     9481 gctggataaa aagctgcgtg accggatgca gcttgaagtg gtggatattc tggagcgcgt
     9541 cggtgtgact tgtgtgatgg tcacccacga tcaggaagag gcgatgacca tggcggggcg
     9601 tatcgccatt atgaatcgcg ggaaatttgt ccagattggc gagccggaag agatctacga
     9661 gcatccaact acccgctaca gcgctgaatt tatcggttcg gttaatgtct ttgaaggtgt
     9721 actcaaagag cgtcaggaag atggcctggt gcttgattcg ccggggctgg tgcatccact
     9781 gaaagtcgat gcggatgctt cagtggtcga taacgtgccg gtacacgtag cgctgcgccc
     9841 ggaaaaaatc atgctttgcg aagagccgcc cgccaatggt tgtaacttcg cggtggggga
     9901 ggtgatacac attgcctatc tcggcgatct ttcggtgtat cacgttcgtc tgaaaagtgg
     9961 gcagatgatt agcgcccagc tacaaaacgc ccatcgtcat cgtaaagggt taccgacctg
    10021 gggcgacgaa gtgcgtttgt gctgggaagt ggacagctgt gtggtgctga cggtttaagg
    10081 agcaaagatg aatacacttg aacctgctgc ccagtcgaaa ccgccgggcg gatttaagct
    10141 gtggttgtcg cagctgcaaa tgaagcatgg gcgcaaactg gtcattgcgt tgccatatat
    10201 ctggttgatc ttgctgtttc tgctgccatt tctgattgtc tttaaaataa gcctggcgga
    10261 gatggcgcgc gctattccac cttataccga actgatggag tgggctgacg ggcaactgtc
    10321 catcactctt aatctcggta attttctgca actgaccgac gatccgctct atttcgatgc
    10381 ttatctccag tcgctacagg tggctgcgat ttcgacattt tgctgtttac tgatcggcta
    10441 tccgctggcg tgggcggtgg cgcacagtaa gccttcgacc cgtaatattt tattactact
    10501 ggtgatcctg ccgtcgtgga cctcgtttct gatccgcgtt tatgcctgga tgggaatatt
    10561 aaaaaacaac ggtgtgctga ataattttct gctgtggctg ggggttatcg atcaaccgct
    10621 gaccattctt cataccaatc tggcagttta tattggcatt gtttacgcct acgtgccgtt
    10681 tatggtactg ccgatttata ccgcgttgat tcgtattgat tattcgctgg tggaagcagc
    10741 gctggatctc ggtgcacgac cgctgaaaac gttctttact gtgatcgtgc cgctgactaa
    10801 aggtgggatt attgccggat cgatgctggt gtttatcccg gctgtgggcg agtttgtgat
    10861 cccggaactg ctcggtggcc cggacagcat catgatcggg cgcgtgctat ggcaagagtt
    10921 tttcaataac cgcgactggc cggtggcctc ggcggtagcg atcatcatgt tgctgctgct
    10981 aattgtgccg ataatgtggt tccacaaaca ccagcaaaaa agcgtgggag aacacggatg
    11041 aataatttac cggtagttcg ttcgccctgg cggattgtga ttttgctgct gggctttacc
    11101 tttctctacg cgccaatgct gatgctggtt atctattcgt ttaacagctc gaagctggtg
    11161 acggtgtggg ccggctggtc aacgcgctgg tatggtgagt tattgcgcga tgacgcgatg
    11221 atgagtgcgg ttggtttaag cctgacaatt gctgcctgtg cggcaacggc ggcggcgatc
    11281 ctcgggacta ttgcggcggt ggtgctggtg cgctttggca ggtttcgcgg atcaaatggc
    11341 tttgccttta tgatcaccgc gccgctggtg atgccagatg tcatcacggg cttgtcgctg
    11401 ttgttattat tcgtcgcgct tgctcatgcc attggctggc ctgcggaccg cggtatgctc
    11461 accatctggc tggcgcatgt cactttttgt acggcttatg tggcggtcgt tatttcttca
    11521 agattgcggg aactggatcg ctcgatagaa gaagcagcga tggatctcgg tgcgacgccg
    11581 ctgaaagtgt ttttcgtcat tacgctaccg atgatcatgc ccgcgatcat ttctggctgg
    11641 ttactggctt ttactttgtc gcttgatgat ctggtgatcg ccagctttgt ttccgggccg
    11701 ggagccacca cgttaccgat gctggtcttt tccagcgtgc ggatgggggt gaatccggaa
    11761 atcaacgccc ttgcaacgtt aattctcggt gcggtcggaa ttgtcggatt tatcgcctgg
    11821 tatctgatgg ctcgcgcaga aaaacagcgg atacgcgata tccagcgtgc aagacatggc
    11881 tgaagacact aaaatttgcc aacctggcta cataatgccg cgcatgccgc ggcattgttt
    11941 tcatggaaga cgaaacgttg ggatttttta agaaaacatc ttcatctcat gctcgcctga
    12001 atgtgcctgc gctggtgcag gtggcggcgc tcgccattat tatgatccgt ggcctcgacg
    12061 tgctgatgat tttcaatacg ctgggcgtgc gcggtattgg cgagttcatt catcgcagcg
    12121 tacaaacctg gagtttaacg ctggtctttt taagcagtct ggtgctggtt ttcattgaga
    12181 tctggtgtgc gttttcactg gtgaaagggc gtcgctgggc gcgctggcta tatctgctga
    12241 cacaaatcac cgccgcaagt tacttgtggg cggcttcgct ggggtacggt tatccggagc
    12301 tgttcagcat tcccggtgaa tcaaaacgtg aaatcttcca tagcctgatg ctgcagaagc
    12361 tgccggatat gctcatcctg atgctgctgt tcgttccctc gaccagtcgg cggttcttcc
    12421 agttgcaata atgtgtataa tcgtcgcccc tgatgatgtg aaggtcaatg tatgcagtgc
    12481 gcactttacg acgcgggtcg ctgtcgttcc tgtcagtgga taacgcagcc gattccagag
    12541 caactctccg ctaaaaccgc cgatcttaaa aatctgctcg ccgattttcc ggttgaggaa
    12601 tggtgcgcgc cggtgtcagg cccggaacaa gggtttcgta ataaagccaa aatggtggtg
    12661 agtggtagcg ttgaaaaacc actgctcggt atgctgcatc gcgacggcac accagaagac
    12721 ctttgtgact gcccgcttta tcctgcctca tttgcgcccg tttttgcggc gctaaaaccg
    12781 tttatcgccc gtgcggggct gacaccctac aacgtggcgc gtaagcgtgg cgaactgaaa
    12841 tacattctgc tgactgaaag ccagagcgat ggcggcatga tgctgcgctt tgtactgcgt
    12901 tctgaaacca aactggcaca actgcgtaag gcgctgccgt ggttacagga acaactaccg
    12961 caactgaaag ttattaccgt caatattcag ccggtacata tggcgattat ggaaggggag
    13021 acggagatct accttactga acaacaggcg ctggcagagc gttttaatga cgtaccgctg
    13081 tggatccgtc cgcaaagttt cttccagact aatccggcgg tcgccagcca gctgtacgct
    13141 accgcgcgcg actgggtacg gcagctgcca gttaaacata tgtgggatct gttctgcggt
    13201 gtgggtggct ttggtttaca ctgcgcgacg cctgacatgc agttaaccgg gatcgaaatt
    13261 gcatcagagg ccattgcctg tgcaaagcag tcagccgctg aactgggctt aacgcgtttg
    13321 caatttcagg cgctggactc cactcagttt gccaccgctc agggggaggt gccggagctg
    13381 gtgctggtta atccgccgcg ccgcggcatt ggtaaaccgc tatgtgatta tctctcaaca
    13441 atggcaccgc gttttattat ctactccagc tgtaacgccc aaaccatggc gaaagatatc
    13501 cgcgaactgc caggttaccg catcgaacgg gtacagcttt ttgatatgtt cccgcacacc
    13561 gcgcactatg aagtgctgac gctgctggtg aagcaataaa aaagccgcag gtgcggcttc
    13621 agattgctga tcaggtgcgc tttgttcatg ccggatgcgg cgtgtacgcc ttatccggcc
    13681 tacaaaatca tgctaaatca atacattgca gagttgatgt gggccggata agcgcagcgc
    13741 atcaggcaat cttacttttg tcatcagtct caattactgc ggaaaccact ggtcattgat
    13801 tttctgatat gtaccgtcag ctttaattgc tgccagcgcg ttattcagtt tttccagcag
    13861 agctttgtta tccggacgta cagcgatgcc cagaccggtg ccgaagtatt gcggatcggt
    13921 cactttttca gtagcaacgc ccagttgcgg attggttttc agccattcgt ttaccaccgc
    13981 tgtgtcacca aataccccat caatacgacc atttttcaga tcgataaagg cattctgata
    14041 actgtcatag gagacagttt tcacttcggg gtgctgatcc tgaatatatt tctggtgcgt
    14101 ggtgccgttt tccatcccaa tacgtttgcc tttcagatcg gcaaacgttt tgtaggtatc
    14161 ttttttggca atcacgacgg ctgagttttc atagtagggc gtggtaaacg atacctgctt
    14221 gctacgctcg ggcgtgatat ccataccgga gattacggcg tcatattttc tgaatttcag
    14281 tgacgggatc aggctgtcga acgcgtgatt agtaaaagta cattctgcct gcatttgttt
    14341 gcacaaggct tttgccagat cgatatcaaa gccgacaatc tcattattag cacctataga
    14401 ttcaaagggt ggataggtgg ctgaaacgcc aaaattgatt ttctctgcgg cagaagcacc
    14461 gaaagtaaag gaagcaagta aagcggcaag aactaacttt ttcatgatgg aactcccgtc
    14521 tgtcaatctt atgatttttg gccgtgtctg cggcatggga taacaatgcc atcaagtgaa
    14581 tttatatgca ataaatatga ttaaataatt taaatgaaat aaaaaaa
//
LOCUS       DAZGLJ010000115        14579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000115 DAZGLJ010000000
VERSION     DAZGLJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.115"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..77)
                     /gene="hokE"
                     /locus_tag="ACHK49_003002"
     CDS             complement(<1..77)
                     /gene="hokE"
                     /locus_tag="ACHK49_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO9263617.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVG"
     gene            46..315
                     /locus_tag="ACHK49_003003"
     CDS             46..315
                     /locus_tag="ACHK49_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263618.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            519..1637
                     /gene="ybdK"
                     /locus_tag="ACHK49_003004"
     CDS             519..1637
                     /gene="ybdK"
                     /locus_tag="ACHK49_003004"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO9263619.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGSGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1703..1951
                     /gene="ybdJ"
                     /locus_tag="ACHK49_003005"
     CDS             1703..1951
                     /gene="ybdJ"
                     /locus_tag="ACHK49_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO9263620.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWQRWFSLAD"
     gene            2016..2384
                     /gene="ybdF"
                     /locus_tag="ACHK49_003006"
     CDS             2016..2384
                     /gene="ybdF"
                     /locus_tag="ACHK49_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO9263621.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2478..3131
                     /gene="nfsB"
                     /locus_tag="ACHK49_003007"
     CDS             2478..3131
                     /gene="nfsB"
                     /locus_tag="ACHK49_003007"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO9263622.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3239..4486
                     /gene="ybdG"
                     /locus_tag="ACHK49_003008"
     CDS             3239..4486
                     /gene="ybdG"
                     /locus_tag="ACHK49_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO9263623.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4554..5930)
                     /gene="pheP"
                     /locus_tag="ACHK49_003009"
     CDS             complement(4554..5930)
                     /gene="pheP"
                     /locus_tag="ACHK49_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO9263624.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6032..9175)
                     /gene="cusA"
                     /locus_tag="ACHK49_003010"
     CDS             complement(6032..9175)
                     /gene="cusA"
                     /locus_tag="ACHK49_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO9263625.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9187..10410)
                     /gene="cusB"
                     /locus_tag="ACHK49_003011"
     CDS             complement(9187..10410)
                     /gene="cusB"
                     /locus_tag="ACHK49_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO9263626.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10426..10758)
                     /gene="cusF"
                     /locus_tag="ACHK49_003012"
     CDS             complement(10426..10758)
                     /gene="cusF"
                     /locus_tag="ACHK49_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO9263627.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10916..12289)
                     /gene="cusC"
                     /locus_tag="ACHK49_003013"
     CDS             complement(10916..12289)
                     /gene="cusC"
                     /locus_tag="ACHK49_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO9263628.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQETRAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSIRLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGG"
     gene            12446..13129
                     /gene="cusR"
                     /locus_tag="ACHK49_003014"
     CDS             12446..13129
                     /gene="cusR"
                     /locus_tag="ACHK49_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO9263629.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            13119..14567
                     /gene="cusS"
                     /locus_tag="ACHK49_003015"
     CDS             13119..14567
                     /gene="cusS"
                     /locus_tag="ACHK49_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO9263630.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         3500 a         3942 c         3627 g         3510 t
ORIGIN      
        1 tccccgacca gaagcgtaaa tcccagcacc gttaaacaca gaactatgac tgccgcaagg
       61 gcatatttcg tcagcatgtt tatattgcct ccatgcgaag aagggactac catccaaatt
      121 gttcaggttt gggtaggtag ccccagggtt gatagaaata tcgtcctggg acttcatctt
      181 tccagacctc agagtaaacc tgaaaccgga aagcttcagg cacccgcagt tatcttaccc
      241 ttgcttagca aaatagctgt ttatttatac agtaagtcct gtttcggact tctcactccc
      301 catttgatca actgaagctg aacccgtttt tttctgcatg cctcaatcgt caaaattctt
      361 cgccaccagc cagccgtgcc ataacttgtc acattgcaaa agtagccaaa ttgcttcagt
      421 caccatcacc aaaaaaagaa acattccaga acttccgcat ctcgcccagc cgctccgact
      481 tctatactga atagaaaacg ccaacttaag agaaacctat gccattaccc gattttcatg
      541 tttctgaacc ttttaccctc ggtattgaac tggaaatgca ggtggttaat ccgccgggct
      601 atgacttaag ccaggactct tcaatgctga ttgacgcggt taaaaatgag atcacggccg
      661 gagaggtaaa gcacgatatc accgaaagta tgctggagct ggcgacggat gtttgccgtg
      721 atatcaacca ggctgccggg cagttttcag cgatgcagaa agtcgtattg caggcagccg
      781 cagaccatca tctggaaatt tgcggcagtg gcacgcaccc gtttcagaaa tggcagcgtc
      841 aggaggtatg cgacaacgaa cgctatcaac gaacgctgga aaactttggc tatctcatcc
      901 agcaggcgac cgtttttggt cagcatgtcc atgttggctg cgccagtggc gatgacgcca
      961 tttatttgct gcacggcctg tcacggtttg tgccgcactt catcgccctt tccgccgcgt
     1021 cgccgtatat gcagggaacg gatacgcgtt ttgcctcctc acgaccgaat attttttccg
     1081 cctttcccga taatggcccg atgccgtggg tcagtaactg gcaacaattt gaagccctgt
     1141 ttcgctgcct gagttacacc acgatgatcg acagcattaa agatctgcac tgggatattc
     1201 gccccagtcc tcattttggc acggtggagg ttcgggtgat ggataccccg ttaaccctta
     1261 gccacgcggt aaatatggcg ggattaattc aggccaccgc ccactggtta ctgacggaac
     1321 gcccgtttaa acatcaggag aaagattacc tgctgtataa attcaaccgt ttccaggcct
     1381 gccgctatgg gctggaaggc gtcattaccg atccgcacac tggcgatcgt cgaccgctaa
     1441 cggaagatac cttgcgattg ctggaaaaaa tcgccccttc cgcacataaa atgggtgcat
     1501 cgagcgcgat tgaggccctg catcgccagg tcgtcagcgg tctgaatgaa gcgcagctga
     1561 tgcgcgattt cgtcgccgat ggtggctcgc tgattgggct ggtgaaaaag cattgtgaga
     1621 tctgggccgg tgactaagta gggaattgcc ttacagaaga atatccctga caatggtgtt
     1681 ttaacttaca tttaacaatg ttatgaaaca ccctttagaa accttgacta ccgcagcagg
     1741 cattttgctg atggctttcc tctcttgcct gctgctgccc gcccccgcac tggggctgac
     1801 gctggcacaa aaactggtga ccatgttcca tctgatggat cttagccagc tttacacttt
     1861 attgttttgt ctgtggtttt tagtgctggg cgctattgag tattttgttc tgcgctttat
     1921 ctggcaacgc tggttctcgc tggcggatta aacgtcgatt atcgccgtac ttcgcggcat
     1981 actttgctta ttctccttcg cctaaaggaa cgtttatgga taagcaatca ctgcacgaaa
     2041 cggcgaaacg cctagccctt gagttaccct ttgtcgagct ttgctggcct tttggcccgg
     2101 agttcgatgt ttttaaaatt ggcggcaaaa tttttatgct gtcgtcggag ctgcgcggcg
     2161 tcccctttat caatctgaag tccgatccac aaaaatccct gttaaatcag cagatatacc
     2221 cgagcattaa gccagggtat cacatgaata aaaagcactg gatttcggtg tatcccggcg
     2281 aggaaatctc cgaagcgtta cttcgcgatc tgatcaacga ttcgtggaat ctggtggttg
     2341 atggtctggc taaacgcgat caaaaaagag tgcgtccagg ctaaagcgga aatctatagc
     2401 gcatttttct cgcttaccat ttctcgttga accttgtaat ctgctggcac gcaaaattac
     2461 tttcacatgg agtctttatg gatatcattt ctgtcgcctt aaagcgtcat tccactaagg
     2521 catttgatgc cagtaaaaaa cttaccccgg aacaggccga gcagatcaaa actctcctgc
     2581 aatacagccc atccagcacc aactcccagc cgtggcattt tattgttgcc agcacggaag
     2641 aaggtaaagc gcgtgttgcc aaatccgctg ccggtaatta tgtgttcaac gaacgtaaaa
     2701 tacttgatgc ctcgcacgtc gtggtgttct gtgcaaaaac cgcgatggac gatgcctggc
     2761 tgaagctggt tgttgaccag gaagatgctg atggccgctt tgccacgccg gaagcgaaag
     2821 ccgcgaacga taaaggtcgc aagttcttcg ctgatatgca ccgtaaagat ctgcatgatg
     2881 atgcagagtg gatggcaaaa caggtttatc tcaacgtcgg taacttcctg ctcggcgtgg
     2941 cggcgctggg tctggacgcg gtacccatcg aaggttttga cgccgccatc ctcgatgcag
     3001 aatttggtct gaaagagaaa ggctacacca gtctggtggt tgttccggta ggtcatcaca
     3061 gcgttgaaga ttttaacgct acgctgccga aatctcgtct gccgcaaaac atcaccttaa
     3121 ccgaagtgta attctctctt gccgggcatc tgcccggcta tttcccctca gattctcctg
     3181 atttgcataa ccctgtttca gccgtcatca taggctgctg ttgtataaag gagacgttat
     3241 gcaggattta atatcccagg ttgaagattt agcgggtatt gagatcgatc acaccacctc
     3301 gatggtgatg attttcggta ttatttttct gaccgccgtc gtggtgcata ttattttgca
     3361 ttgggtggta ctgcggacct tcgaaaaacg tgccatcgcc agttcacggc tttggttgca
     3421 aatcattacc cagaataaac tcttccaccg tttagctttt accctgcagg ggattatcgt
     3481 caatattcag gcggtattct ggctgcaaaa aggcaccgaa gcggcagata ttctgactac
     3541 ctgcgcgcag ttgtggatca tgatgtatgc gctgctttca gtcttctcgt tgctggatgt
     3601 tattttgaat ctggcgcaga aattcccggc ggcatctcag ttaccgctga aagggatatt
     3661 tcaggggatt aaactgatcg gcgcaatcct ggtcggcatt ttgatgatct cgctgctgat
     3721 tggtcagtcg ccagcgattc tgatcagcgg tcttggtgca atggctgccg tgctgatgtt
     3781 ggtttttaaa gatccgattc ttggtctggt ggcaggtatt cagctttccg cgaacgatat
     3841 gctgaaactg ggcgactggc tggagatgcc gaaatacggc gcggatggcg cggtgatcga
     3901 tattgggtta accaccgtca aagtgcgtaa ctgggacaat accattacca ctattcccac
     3961 ctggtctctg gtttctgact cctttaaaaa ctggagcggg atgtcagcat ctggcgggcg
     4021 acgtattaag cgcagtatca gtattgatgt caccagtatt cgttttcttg atgaagacga
     4081 aatgcaacgt ctgaataaag cgcacttgtt aaaaccttat ttaaccagcc gccatcagga
     4141 aattaatgag tggaatcgcc agcaaggttc tacggagtcg atattaaacc tgcgccgaat
     4201 gaccaatatt ggaacctttc gtgcctatct gaacgaatat ctgcgtaacc atccgcggat
     4261 tcgtaaagat atgaccttaa tggtacgcca actggctcca ggtgataacg gtttaccgct
     4321 cgagatctac gcgtttacta acaccgtggt gtggctggaa tatgaaagca ttcaggctga
     4381 tatattcgat cacatatttg cgattgtcga agagtttggt ctgcgacttc atcagtcgcc
     4441 aaccggcaat gatattcgct ctctggcggg tgcatttaag cagtaattaa aaaaaccgct
     4501 ctcatcgaat ggatgagagc ggacatcgtt ttgtcggatg tagcgtgaat gccttatttc
     4561 cgacgcagcg ttttaaatgc cacaaacagg aatacaatcc acaccggcag caggatcgct
     4621 gacaagcgca tatcatccat cgtgcacatc agcagcaaaa tcatgccgag gaaggcaatg
     4681 caaagatagt tgccgaacgg atagagcagc gccttaaact gtgtttcacg cccctgacgt
     4741 cgcatcgctg cacgaaaacg cagatgcgcc agacagatca taatccagtt caacagcagc
     4801 gttgctacca ccagcgccat cagcagacca aacgcttttt gcggcagcag atagttgatt
     4861 aataccacca gcgaagtgat cgctccggaa agcatcagcg agttaatcgg cacaccgcga
     4921 cggctgacgc gagtcaaaaa ctttggcgca ttaccctgca cagaaaggcc aaacagcatg
     4981 cggctgttag agtaaacccc gctgttatac actgacagcg atgctaccag aatgacgaag
     5041 ttcagcgcag aagctaccac gttgctgtcg agattatgga aaatcatcac aaacgggcta
     5101 ctgttggatt tcacttccac ccacggatag agcgccagta aaaccaccag tgaaccgatg
     5161 taaaacagca ggatgcgata caccacctga tttaccgctt ttggaatgct tttttccgga
     5221 tcgcgcgctt cagcggcagt aatcccaatc agctccagac cgccgaagga gaacataatt
     5281 accgccagcg acaaaatcag cccattccag ccggtggcaa agaaaccacc gtagcgccag
     5341 aggttgtcga tactggcttt ctcgccgccg tgaccagaaa acagcagcca caggccaaag
     5401 ccgatcatac cgatgatcgc cagcacttta atcagcgcaa accagaactc ggtttcacca
     5461 tataagcgca cgttcaccag gttaacggcg ttgatgataa taaagaaggc ggcagcccaa
     5521 atccacgttg gaacatccgg gaaccagtac tgcatataga tgcccgcagc ggtcagctct
     5581 gccattccca ccagcacgaa cattacccag tagttccagc cagagaggaa gcccgcaaac
     5641 ggtccccagt atttataggc aaagtgggca aatgaaccgg ataccggctc ttcgaccacc
     5701 atctcgccaa gctggcgcat aatcaggaaa gcgatgatcc cggcgacgcc gtagcccagc
     5761 aatacagccg gacccgccat ctgaatcgcc gggccaatgc caagaaacag accagtacca
     5821 attgcgccac ccagcgcaat cagttgaata tgacggttat gtaatccgcg atgaagcgtc
     5881 ggctcttgat tcgacgcagt atcttccgat acggttgacg cgtttttcac gcctttcccc
     5941 tgtgtgtctt ttttgttgag gggcaccttt taacatttag tgcccatcgt cgcaagacac
     6001 aatccacacg gttaaacggg gtatcctgct tttatttccg tacccgatgt cggtgcagcc
     6061 acatcagctt atacgccgcc gggataataa acagcgacag caaaggtgcg gtgatcatgc
     6121 cgccaatcat cggtgcggca atccggctca tcacctctga accagccccc gttccccaca
     6181 gaatcggcag cagaccggcg ataatcaccg ccaccgtcat cgctttcggg cgcacgcgca
     6241 ggaccgcgcc gtgatataac gcctcatcca gcttctgctc gctgaatgtt tgtggattat
     6301 tcaacgacgg ctcggcctct atggcgtgac gtaaatacat cagcatcacc acgccaaatt
     6361 cggcggcgac cccggcgagg gcgataaagc cagtgcccgt cgccacggaa agatgaaagc
     6421 ccatccacca cagcaaccag atgccgccca ccagcgcaaa cggtacgctg ctgataatca
     6481 gcaacgcttc gcccacccga cggaacgcca gatacaacag cacgaagata atcatcaacg
     6541 tcatcggcac catgagttta agcttatggt tggcgcgctc cagtagctcg aactgcccgg
     6601 agaatgccac gctggtgccc ggttttaact gcactttttc agctatcgct ttttgcaaat
     6661 cgtgaaccac cgacaccatg tcacgatcgc gggcatcgat ataaatccag ctcgtcgggc
     6721 gcgcattctc ggttttcagc attgacggtc cggtagagac tttaatgtcg gccacgtctg
     6781 ccagggtgat ttgctgcttc atcggcgtca ggatcggcaa ctggcgcagt gcctgcggac
     6841 tatcgcgcca gctttgcgga taacgcagat taattggata acgggcaatc ccttccaccg
     6901 tttcgccaac catcgccccg cccaccgcag aagtcacaaa caactgcaca tccgccaccg
     6961 tcataccgta acgtgcggct ttttcacggt taatctcaac gttgatatag cgcccacctt
     7021 ccagacgctc ggctagagca gaagccacgc ctggcaccgt tcgcgctact tcttcaattt
     7081 gctcagccat cgcgtcgata tccgccagca cagtgccgga aactttaatg ccgatggggc
     7141 ttttaatgcc ggttgagagc atatcgatac ggttacgaat tggcggcacc cacagattcg
     7201 ccagccccgg cagacgcacg gtgttatcca gttcctcaat gattttgtcc attgtcatgc
     7261 ctggccgcca ctgctcctgc ggcttaagct ggatggtcgt ttctaccatc tccagcggag
     7321 cagaatcggt ggcggtttcc gctttcccgg ttttgccaaa tacccgcgcc acttcaggta
     7381 cgctcataat tagcttgtcg gttttttgca gcatactcgc cgcctctgct gcggaaatcc
     7441 ccggcagcgt cgatggcata tacaacaggt cgccttcatt gatctgcggt aaaaattccc
     7501 cgccaacttt attgagcggc cagagaaccg tcagcaccga aagcgctgcc accaacagcg
     7561 tggttttcgg ccagtgcagt actttcagca acagcggatg ataaacacga atcaaaaagc
     7621 gattgagcgg gttactgctt tccggcggaa ttttgccacg gatccagtaa cccatcagga
     7681 tagggatcac tacgatcgcc agcagcgccg cacccgccat cgcatacgtt ttggtgaacg
     7741 ccagcgggcc aaacagacgc ccttcctgcc cttccagggt gaagatcggg ataaacgaca
     7801 acgtgataat cagcagactg ataaatagcg ccggacccac ttcaacagat gcatcggtga
     7861 tcacctgcca gcgcgtttta ttatccagcg tggcgtcagg atgctggtgc tgccactctt
     7921 ccagccgttt atgtgcattt tcgatcatga cgatagcggc atcgaccatc gccccgacgg
     7981 caatcgcaat gccgcccaac gacataatat tggcattcag tccctggaag tgcatgacaa
     8041 taaaagcaat acacaacccc agcggcaacg aaataatcgc caccagcgcc gagcgcacat
     8101 gccagagaaa cagcgcacag actaccgcca ccacaataaa ctcttccagc aacttgccgc
     8161 tgaggttgtc gatggcgcgg tcaatgagct ggctgcgatc gtatgttgta actatctcca
     8221 cgccttccgg cagactactt ttcagcgttt ccagtttgtc cttcacggcg gcgatcactt
     8281 ctcgggcgtt tttgccggat cgcaggatca ccaccccgcc cgccacttcg ccttcgccgt
     8341 ttagttcggc aatgccccgg cgcatctccg ggccaatctg gaccttcgca acatcgcgca
     8401 gataaacggg cacgccattt tcactggctt ttaaaacgat gtgattaaag tcgtcgagcg
     8461 tttgcagata gccgctggcg cgcaccatat attccgcttc cgccagttcg atcgacgaac
     8521 cgcccgcttc ctggtttgaa gcatccagcg cgctttttac ttcggcgaga ctgatgccat
     8581 actgcgccag gcgctgggga tcgataacca cctgatactc tttcaccaca ccgcccaccg
     8641 acgccacttc cgcaacgtca gggatggttt tcagctcata tttgagaaac cagtcctgca
     8701 atgagcgtaa atcggccaga tcgtgcttac cgctgcgatc caccagtgca tattcataga
     8761 tccagccaac acccgtggca tctggtccca gctcggcgct gactcccgca ggcagcttac
     8821 cctgtacctg gttgaggtac tccagcaccc gcgaacgcgc ccagtacgga tcggtgccat
     8881 cttcgaaaat gacatacaca taagagtcgc caaactgtga gaaaccgcgc acagtcttcg
     8941 cgccaggcac cgacaacatg gtggtggtta gcggataagt cacctgattt tcaacgattt
     9001 gtggtgcctg accgggatag ctggttttaa taataacctg cacatcggag agatccggca
     9061 gcgcatccac tggcgtatta atgatggtcc aggtgcccca gatgctcaga aacaacgcgc
     9121 ccatcagcac caaaaaacgg ttcgccaccg agcgacgaat aatccattca atcattggtt
     9181 attccctcaa tgcgcatggg tagcactttc agagcgcatc cgctccagtg cgccagaaat
     9241 attggcttcg gaatcaatca ggaacaggcc gctggaaacc accttttcac cttccgccag
     9301 accagagcgt aatgcggtga cgccttgcga tgcctggaaa acagcaacgc gtttcggtac
     9361 aaagcgcccg tcggcatcaa cggtaatcac ccgctgttcg ctgccggtat caatcagcgc
     9421 ttgtgacgga atgagcagca tcggttcgct ggcggtgttg agttgcaacc aggcgttcat
     9481 gcccggtttt agcgcctcgt cggcgttgtc gacttccaga cgcagctgca gcgtgcgggt
     9541 cgcggcatcc acgccaggta gcagcgtcca tttgcggatg gtgagtgttt tatccggtcg
     9601 cgccggaacg gtgagggtaa actgcgaggc atctttcacc agccaggcga tggactccgg
     9661 gatcgcagca gtgacccaca ccgggtccat accctgaatt ttcgcgacca cgttatcttt
     9721 ggcgatattc attcccgcgc gcagatcaaa cgcggtgatc acgccatcaa tgggcgcttt
     9781 gagcgtaaag cgagtctgga ttttttgcgt ggcgatcagg cggcgaatat ccgcctccgg
     9841 cattcccgcc agtcgcagcc gctcaagaat gccttcagtc tgggtcgccg taccgccggt
     9901 ttcgcgcagc agtaaatact cactctgcgc ttccacccag tcaggaatgg tcaggtcgag
     9961 aagcggtgtg cccttttgta ctttatcgcc cacggtaagc ggatacacct tgtcgataaa
    10021 cccggcagcg cgagcctgca caatggcata ctgatactcg ttgtaactga cattcgccgg
    10081 gaaactctgg gcaaaagtca gcggcccgcg cgtgacggta gccgttttca cccccagatt
    10141 ctgcgtctga gtcgggtcaa tgcgcacacc agacgcagaa ctctcttcat cggcatattt
    10201 cggcaccaga tccatatcca taaacggcga tttacctggt ttatcgaacc gcgtattggg
    10261 gtacatcggg tcgtaccaga ataagatttt acgttctgcg gtcgacgttt tttctgcggg
    10321 tggttcagcc tttgcaaacc aggtaaaacc tgccgcagaa ataataccgc ccgcgatcat
    10381 gctgccgata ataagcgcga tttttttcat ctcattaaac ctgggttact ggctgacttt
    10441 aatatcctgt aataaagaaa ggttgccctg ctggacaaaa ttaaacgcca ctttgtcgcc
    10501 agttttaatt tcactcattt tcgtctgcgg agtgatggta aagcgcatgg tcatctccgg
    10561 ccagttcacg gcagcaatcg gatcgtgatg gatggtgatt tttttgcttt ccagatcaat
    10621 accctttacc acgccagtgg cgctaataac ctgcggttgt acttcgctca tggtttcatg
    10681 atgatgttcg ttagcctggg cattaaagcc aataacggta aacagactga acattgcgac
    10741 ttgcagtgct tttttcattt attctctcct ggagttaaaa gtcatactct aaataattcg
    10801 agttgcagga aggcgacaag cgagtgaatc cccaggagct tacataagta agtgactggg
    10861 gtgagcgaac gcagacgcag cacatgcaac ttgaagtatg acgagtatat tgctgtcaac
    10921 cgccacctaa ggcggtatac aaagaaattt cgttaacctg acgggcataa ttcaggtcga
    10981 gtaaagtttg tcgggttgca aataaagaac gctcggcatc cagcacttcc agataactta
    11041 ctgcgccgtg ctgatataat gcccgcgccc gttgcaaagt aatttgcagc gacgccagat
    11101 aacgctgctg ggcgctgatt tgatcgttca ggctttgacg taatgccagc gcatctgcca
    11161 cttctttaaa ggcgttctgg attttctgtt cataattcac taccgactgc tgctggcgaa
    11221 tttcggcgat atccagattg gcctggttgc gtccggcatt aaaaatgggg atctcaattt
    11281 taggaataaa attccacatc ccgctgctgg cgttaaataa tgacgacaga tcgctgctgg
    11341 cggttgatat tccgctggtc agccttatag atgggaaaaa tgccgcgcgt gcggccccaa
    11401 tattggcatt agccgccatt aacgcgtgtt cagcttccat aatatccggg cgctgcaata
    11461 agatttgcga cgacaatccc gccggtaatt taacgctttg caggctgtcg ctgtttactg
    11521 tctgcgcttg cggcagcttg ccgtagcttc ccaataacag ttgcaatgca ttattcgcct
    11581 gcgccagttc cccctgacgt ttagcgatgt cgctgcgggt actttcaatc accccgcgag
    11641 cctgttccag cgccagaaca ttactgctac ctgtcaacag ttgtttttcg acaaacgcat
    11701 atgactgctg ataattacgc agcgtttctt cggctatttg caattgcgca tacgctagct
    11761 gctgattgaa ataactttgc gcgacattag aaaccagcaa aatatgcacc gcgcgctggg
    11821 cttcctcagt ggctaaataa ttttggcgct cggcttcgct catgttcttt aagcgaccga
    11881 aaaaatcgag atcaaagctg gcgttaaggc cggtcgaaaa ctcccgtgtc gtggcggtat
    11941 tccctttaag attaccgctc cagctgccgc tgccctcgcc attgagctgt gggtagcggt
    12001 cggcatcggt cagacgatat tgcgcccgcg tttcctgcac tttcagcgtc gccatgcgca
    12061 aatcccggtt attcaccagc gcctcgctaa tcagcgtctt tacctgatta tcaacaaaaa
    12121 aggtgcgcca gcccacgttc tggtagttat ctgctgcgtt aaccaagccg ttctggctga
    12181 gtgagaactg ctgcggcacg ggcattgccg gacgctgata atccggtgcc agtgaacaac
    12241 cggttagcgc aagggccaca caaaatggca gaagtttaca aggagacata ggctcataat
    12301 ttctggtgat tttataccgc caactttact cgccaggctc tgattttccg gtgacaggaa
    12361 aatgacaaaa ttgtcatttt gccaataagc gattgccatc tgatcccgct actctagaat
    12421 tgcccgggca acatgcggag gaaaaatgaa actgttgatt gtcgaagatg aaaagaaaac
    12481 cggagaatac ttgaccaaag ggttaaccga agccggtttt gtggtcgatt tggccgacaa
    12541 cgggctgaat ggctaccatc tggcgatgac cggtgattat gatctgataa tcctcgatat
    12601 tatgctgccg gacgtgaacg gctgggatat cgtgcgcatg ttgcgttccg ccaataaagg
    12661 gatgccgatt ctgttgctta ccgcgcttgg caccattgaa catcgcgtca aggggctgga
    12721 gttgggggca gatgactacc tggtgaaacc attcgctttt gctgaactgc tggcgcgggt
    12781 gcgcactctc ctgcggcgcg gggcggcggt gattatcgaa agtcagtttc aggttgccga
    12841 tctgatggtc gatctcgtca gccgcaaagt cacccgcagc ggcacgcgca tcactctgac
    12901 cagtaaagag tttactctgc tggagttttt cctccgccat cagggcgaag tgctgccccg
    12961 ctcgcttatc gcctcgcagg tgtgggacat gaatttcgac agcgacacta acgccattga
    13021 tgtagcggtg aagcggctgc gcggcaaaat cgacaacgac tttgagccaa agctaattca
    13081 gaccgtgcgc ggcgtgggtt acatgcttga ggtgccggat ggtcagtaag ccatttcagc
    13141 gcccgttttc gctggcaacc cgcctgacct tttttatcag cctggccacc atcgcagcgt
    13201 ttttcgcctt tgcatggatc atgatccact cagtaaaagt gcattttgcc gagcaggata
    13261 ttaatgattt aaaagaaatt agcgccacgc ttgaacgggt acttaatcat cctgacgaga
    13321 cgcaagcccg acgcttaatg acgctggaag atatcgtcag tggttattcc aacgtgttga
    13381 tctccctggc agatagccac ggcaaaacgg tgtatcactc ccccggtgcg cctgatatcc
    13441 gcgagtttgc acgtgatgcc atacccgata aagacgcgcg gggtggcgag gtgtatctcc
    13501 tttccggccc gacgattatg atgccaggcc acggtcacgg gcatatggaa cacagcaact
    13561 ggcggatgat taacttgtcg gttggcccgt tggtggacgg caaaccgatt tatacgctct
    13621 acatcgcgct ttcgatcgat tttcatcttc attacataaa tgatttgatg aataaactta
    13681 ttatgaccgc atcggtaatc agcatcctga tcgtctttat cgtactgttg gcggtacata
    13741 aaggtcacgc gccgatccgc agcgtcagcc gtcaaatcca gaatatcacc tcgaaagatc
    13801 tcgacgttcg ccttgacccg cagaccgtgc ccattgagct ggaacagctg gtactgtcgt
    13861 tcaaccatat gatcgagcgt attgaggatg tctttacccg ccagtccaat ttctcagcgg
    13921 atatcgctca cgaaattcgc acgccgatta ccaatctcat cacgcaaacg gaaatcgccc
    13981 tcagccagtc tcgcagccag aaggagctgg aagatgtgct ctactctaat ctcgaagagc
    14041 tgacgcgaat ggcgaaaatg gtcagcgata tgctgtttct cgctcaggcc gataacaacc
    14101 agctaatccc cgaaaagaaa atgctcaacc tggcggatga agtcggcaaa gtgttcgatt
    14161 ttttcgaggc gttagcggaa gatcgcggcg tggagttgcg atttgttggc gacgaatgtc
    14221 aggttgcggg cgatccgctg atgctgcgtc gggcgttaag caacctgctt tctaatgccc
    14281 tgcgttatac gccgcccgga gaggcaattg tagtgcgctg ccagacggtc gatcaccagg
    14341 tgcaagttac cgtcgaaaac cccggtacgc ccattgcgcc cgagcactta ccgcgattgt
    14401 ttgaccgttt ctaccgcgtt gacccttccc gccagcgaaa aggcgaaggt agcggcattg
    14461 ggctggcgat agtgaaatcg attgttgtcg cgcataaagg cacggttgca gtaacgtcag
    14521 atgcgcgggg gacaaggttt gtgattatgc tgccggagag agagtgatta ttaagttca
//
LOCUS       DAZGLJ010000116        14282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000116 DAZGLJ010000000
VERSION     DAZGLJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.116"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(234..1286)
                     /gene="fbaB"
                     /locus_tag="ACHK49_003016"
     CDS             complement(234..1286)
                     /gene="fbaB"
                     /locus_tag="ACHK49_003016"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO9263631.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            1543..2820
                     /gene="yegT"
                     /locus_tag="ACHK49_003017"
     CDS             1543..2820
                     /gene="yegT"
                     /locus_tag="ACHK49_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO9263632.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            2817..3821
                     /gene="yegU"
                     /locus_tag="ACHK49_003018"
     CDS             2817..3821
                     /gene="yegU"
                     /locus_tag="ACHK49_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO9263633.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            3818..4783
                     /gene="yegV"
                     /locus_tag="ACHK49_003019"
     CDS             3818..4783
                     /gene="yegV"
                     /locus_tag="ACHK49_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO9263634.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4757..5503)
                     /gene="yegW"
                     /locus_tag="ACHK49_003020"
     CDS             complement(4757..5503)
                     /gene="yegW"
                     /locus_tag="ACHK49_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO9263635.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5555..6373)
                     /gene="yegX"
                     /locus_tag="ACHK49_003021"
     CDS             complement(5555..6373)
                     /gene="yegX"
                     /locus_tag="ACHK49_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO9263636.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            complement(6438..7238)
                     /gene="thiD"
                     /locus_tag="ACHK49_003022"
     CDS             complement(6438..7238)
                     /gene="thiD"
                     /locus_tag="ACHK49_003022"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO9263637.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(7235..8023)
                     /gene="thiM"
                     /locus_tag="ACHK49_003023"
     CDS             complement(7235..8023)
                     /gene="thiM"
                     /locus_tag="ACHK49_003023"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO9263638.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(8058..8154)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(8246..8518)
                     /gene="rcnR"
                     /locus_tag="ACHK49_003024"
     CDS             complement(8246..8518)
                     /gene="rcnR"
                     /locus_tag="ACHK49_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO9263639.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            8639..9463
                     /gene="rcnA"
                     /locus_tag="ACHK49_003025"
     CDS             8639..9463
                     /gene="rcnA"
                     /locus_tag="ACHK49_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO9263640.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVVSKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWHGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            9682..10020
                     /gene="rcnB"
                     /locus_tag="ACHK49_003026"
     CDS             9682..10020
                     /gene="rcnB"
                     /locus_tag="ACHK49_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO9263641.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(10102..11136)
                     /gene="yehA"
                     /locus_tag="ACHK49_003027"
     CDS             complement(10102..11136)
                     /gene="yehA"
                     /locus_tag="ACHK49_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO9263642.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSSVTLLNITNSSKRYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(11150..13630)
                     /gene="yehB"
                     /locus_tag="ACHK49_003028"
     CDS             complement(11150..13630)
                     /gene="yehB"
                     /locus_tag="ACHK49_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO9263643.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAASYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(13646..14263)
                     /locus_tag="ACHK49_003029"
     CDS             complement(13646..14263)
                     /locus_tag="ACHK49_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO9263644.1"
                     /translation="MVIYGTRIIYPAENKEVMVQLMNQGNRSSLLQAWIDDGDTSLPP
                     EKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKESIFYLNVLDIPPNSPEQEGK
                     NALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNGLVIKNDSANWVTISDVKAN
                     NVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGNYISDKI"
BASE COUNT         3472 a         3617 c         3393 g         3800 t
ORIGIN      
        1 caaaagcgac aatgtgaaaa ttgttcgagg agtaaaaggc aaaaaaaaac ggcctcccga
       61 tagggaagcc gtagcaaagt gcgcgtgttt ttatgccgga tgcggtgtaa acatacgtgg
      121 cagtgctatg taggcatgat aagacgcttc cgcgtcgcat caggcatagg ttgccggatg
      181 cagtgtaaac gccttatccg tcctacgggg tggtgctgtt cgtatgaggc gtatcaggca
      241 atagtaattt tgctatcgag ataaacgtcc tgcacggcat taatcagttt cacgccgtca
      301 gccatcgatt tcttgaacgc cttacgtcca agaatcagcc ccattccgcc agcgcgtttg
      361 ttgataaccg cagtacgcac tgcatcgctg aggtcagttt caccgcccgc agcaccgccg
      421 gagtttatca acccagcccg acccatatag cagttagcta actgataacg caccagatca
      481 atcgggtttt cgctggtcaa tttgctgtaa acacgatcgt cggtgtaacc gtaattaatt
      541 gctttatagc cgccgttatt ttccgccatt ttttgtttga cgatatctgc accgatggtt
      601 gccgccagat ggtttgcctg accggtcagg tcggcggaaa catggtaatc aacgccatct
      661 ttcttaaagg cggagttacg caaataggcc cacagcactg tcaccatacc cagctcgtgc
      721 gcacgttcaa aagccgcaga aatttcttca atctggcgac gtgactcttc cgagccaaaa
      781 tagatagtcg caccaaccgc aaccgcgccc atgttgaacg cctgctccac gctggcatac
      841 agcgtttgat cgtaggtatt cgggtaactt agcgtctcgt tgtgattaag tttgacgagg
      901 aatggaatgc gatgcgcata acgccgcgat accgacgcca gcacgccgta agttgacgcc
      961 acacagttac agcccgcttc gatcgccagt tcaacaatgt ttttcgggtc aaagtagagc
     1021 gggttagcag caaatgaagc tccggcagag tgctcaacgc cctggtcaac cggcagaata
     1081 gaaagatatc ctgtgccagc cagacgcccg gtgttgtaca acgtctgcat attacgtaac
     1141 accgctggcg ggcgattatt gtcaatcatt acgcggtcta cgtagtcatg tccggggaga
     1201 taaagctggt cagaaggaat tgtcatacaa cggtgctgta aaaggttgtc ggcgtctttg
     1261 ccaagcaact gcgcaatatc tgtcattact atgctcccgt aaattccgat tggatatcgg
     1321 ctatggattg tcctggcccg ccttttgcgg gcaatcataa tcctggtcgt tacgggcgag
     1381 tttttccatc ttttggatgt tttttcagcg ttttctgttg gctcgattca tcagaaaaaa
     1441 tgttagcgcg gtcaagtttt caccgcaaag gtatttaaaa ggtattatta agtggtattg
     1501 tcatcgcgta ccttacatta cctgtcatga aggaattaaa agatgaaaac aacagcaaag
     1561 ctgtcgttca tgatgtttgt tgaatggttt atctggggcg cgtggtttgt gccattgtgg
     1621 ttgtggttaa gtaaaagcgg ttttagtgcc ggagaaattg gctggtcgta tgcctgcaca
     1681 gccattgcgg cgatcctgtc gccaattctg gttggctcca tcactgaccg ctttttctcg
     1741 gcgcaaaaag tgctggcggt attgatgttc gcaggcgcgc tgctgatgta tttcgctgcg
     1801 caacagacca cttttgccgg gttcttcccg ttactgctgg cctactcgct aacctatatg
     1861 ccgaccattg cgctgactaa cagtatcgct tttgccaacg tgccggatgt ggagcgtgat
     1921 ttcccgcgca ttcgtgtgat gggcactatc ggctggattg cctctggtct ggcatgtggt
     1981 ttcttgccgc aaatgctggg gtatgccgat atctcaccga ctaacatccc gctgctgatt
     2041 accgccggaa gttctgctct gctcggtgtg tttgcgtttt tcctgcccga cacgccgcca
     2101 aaaagcaccg gcaaaatgga cattaaagtc atgctcggcc tggatgcgct gatcctgctg
     2161 cgcgataaaa acttcctcgt ctttttcttc tgttcattcc tgtttgcgat gccactggcg
     2221 ttctattaca tctttgccaa cggttatctg accgaagttg gcatgaaaaa cgccaccggc
     2281 tggatgacgc tcggccagtt ctctgaaatc ttctttatgc tggcattgcc gtttttcact
     2341 aaacgctttg gtatcaaaaa ggtattattg cttggtctgg tcaccgctgc gatccgctat
     2401 ggcttcttta tttacggtag tgcggatgaa tatttcacct acgcgttact gttcctcggt
     2461 attttgcttc acggcgtaag ttacgatttt tactacgtta ccgcttacat ctatgtcgat
     2521 aaaaaagccc ccgtgcatat gcgtaccgcc gcgcaggggc tgatcacgct ctgctgccag
     2581 ggcttcggca gtttgctcgg ctatcgtctt ggcggtgtga tgatggaaaa gatgttcgct
     2641 tatccggaac cggtaaacgg gctgactttc aactggtccg ggatgtggac tttcggcgcg
     2701 gtgatgattg ccattatcgc cgtgctgttc atgatttttt tccgcgaatc cgacaacgaa
     2761 attacggcta tcaaggtcga tgatcgcgat attgcgttga cacaagggga agttaaatga
     2821 aaacagaacg tattctcggt gctctttatg ggcaggcgtt aggggatgcg atggggatgc
     2881 cctccgagct ttggccacgc agccgcgtta aagcacactt tggctggatt gaccgttttc
     2941 tgcctggacc aaaggagaat aacgcggcct gttattttaa ccgcgccgaa ttcaccgacg
     3001 atacctcgat ggcgctgtgt ctggcggatg cgttactgga acgtgaaggc aagatcgatc
     3061 cggatctgat tgggcgtaat attctcgact gggcgctgcg tttcgacgcc tttaacaaaa
     3121 acgtactagg tccgacctcg aagattgcgc ttaacgccat tcgcgacggt aaacccgttg
     3181 ctgaactgga aaacaacggc gtgaccaacg gcgcagcgat gcgcgtctcg ccattaggtt
     3241 gtttgcttcc ggcgcgtgat gttgattcct ttattgatga tgtggcgctg gcgtcgagcc
     3301 cgacccataa atccgatctg gcagttgcag gtgcggtagt catcgcatgg gcgatttctc
     3361 gtgccattga cggagaaagc tggtcagcga ttgttgattc cctgccttca attgcgcgac
     3421 atgcacagca aaaacgcatc actaccttca gcgcctcact ggcagcacgt ctggagattg
     3481 cgctgaaaat tgtgcgcaat gccgacggca ccgaatccgc cagcgaacag ctttaccagg
     3541 tcgttggcgc aggtaccagc actattgagt ccgttccgtg cgccattgcg ctggttgaac
     3601 tggcacaaac cgacccgaat cgctgcgccg tcctgtgcgc taaccttggc ggcgacacag
     3661 acaccatcgg cgctatggcg acggcgattt gcggcgcgtt gcatggcgtt aacgctatcg
     3721 atcctgcatt aaaggcggaa ctggatgcgg taaatcagct tgatttcaac cgctatgcca
     3781 cagcgctggc gaaatatcgt caacaacggg aggcggtatg agcggcgctc gattacacac
     3841 gctgctgcct gaattaacct cgcgtcagcc ggtgatggtc gtcggcgcgg cggtcattga
     3901 tgtgatcgcc gacgcttatg ccctcccctg gcgtgggtgc gatatcgaac tgaagcagca
     3961 gagcgttaac gttggcggct gcgcactgaa tattgccgtg gcgttaaagc gcctcggcat
     4021 cgaagcgggt aatgccttgc cgctcggtca gggcgtgtgg gcggagatta ttcgcaaccg
     4081 gatggcgaaa gaggggttaa tcagcctgat cgataacgcc gaaggcgata acggctggtg
     4141 tctggcgctg gttgagccgg atggcgaacg cacttttatg tcattcagcg gtgtggaaaa
     4201 tcagtggaat cgccagtggc tggcgcgatt aaccgttgcg cctggcagcc tgctctattt
     4261 ttccggttat caactggcct cgccctgcgg cgaattgtta gtggaatggc tggaaaagct
     4321 gcaagacgtg acgccgttta tcgattttgg cccacgtatc ggcgatatcc cggacgcatt
     4381 actggcgcgg atcatggcct gtcgaccttt agtatcactc aatcgtcaag aggctgagat
     4441 tgccgccgaa cgttttgctt tatccgcaga gataacaaca cttggcaagc aatggcagga
     4501 gaaatttgcc gcgccgttga tcgttcgtct cgataaagag ggtgcatggt atttcagcaa
     4561 cgacgcttct ggctgcattc cggcatttcc aacgcaagtt gtagacacca ttggggcggg
     4621 cgacagtcat gcaggtggcg tacttgccgg gctggcctct ggtctgccac tggcggatgc
     4681 cgtattactg ggcaatgcag tggcgtcgtg ggttgtcggg catcggggcg gtgattgtgc
     4741 gccaacgcgc gaggaactac tcctcgcaca caaaaacgta tagatcgctg cgacagtggc
     4801 taatgctgta ctcaataggc cgttgctgtt ggtcaagcgc cacttgcttg atcaccagca
     4861 ccggtatttt gctatctaac tgaatatgtg actgaaactc ggcatccggc atccgggcgc
     4921 taacgcggga acgcgtacgt tgtgggtaaa tgtgctggct gcggaaatag tcataaagtg
     4981 aaatcccgat ggcatcaaca tcatgaatta aatgcgccgg aacccacgat tcctcaatcg
     5041 ataccgcttc ttcatcgaca tagcgaatac gcttaagcaa aaagacatca cttcctgctt
     5101 cgaccgccag ctgctgcgcg acttcttcgg gacatttcac gacacgcttg ttaacccata
     5161 gcgtatcggg ctttttcccg cgcaatacca cctgctgaga aaaacctcgc gcttctttca
     5221 acgagtattc gaagatgttg ttgatctgcg tgccgtaacc gcgcgaacgc gtcaccacac
     5281 cctcttcttc cagcgcctgc atcgccttgc gcaccgtaat gcgcgaaacg ccggttaact
     5341 gacttaaatc acgctcaccg ggcaaaatat tgccatgctc cagcaccccg ctgcgcacgg
     5401 cattttttac cgtttcggca aacttaatat aaagcggcgt gttatccgcc gctaaaatac
     5461 gctcgttcag ttgggcaatc agctgggtat gcgcttgttc catttgtctt tttcctgccg
     5521 gggagtctgg tgggagtata tcactgcttc ttatttaagg cgtttcttta atcccatcaa
     5581 cgaatgcctg caactcctcc accgtgccat gaaatacatt aaaatccacc ggaccattga
     5641 tgccatctac ctgtccacgg tcggaatgct gccagaagcg ccaggccatg ccgtcattgt
     5701 ccggacgacg ttgataatag tgagccaccc accacggata ttcattgaaa tagcccgcca
     5761 gattggtgtg ataaaaaacg gctcctgagt aaataatcgg cttttttccc gtactttttt
     5821 cgaccatttt tagccactga cttactcgtt tgcgtaattc ttttgccgat aattttcccc
     5881 gttcttctac gtccagtacc gcagggaaat cgccttgtga gaaatccacc gtttgcagaa
     5941 ataatcgtgc ctgaaccggc gctgctaccg acggggagaa ataatgatac gccccgcgca
     6001 gcaggccatt ttcgcggctt agttgccagt tacgcgaaaa atacgggtcc accagctttt
     6061 cgccttccgt cgccttaata aaagcaaact gtaagcggat accgttgtcg cgcatttttg
     6121 ccacccgctg ccagtcgatc cgctcctgcc agcgagaaac atctattccg tgaatggtgt
     6181 aactggcagg aatgcgaata gcgaaagatt taaccgggcg ataaccgtag aaatggatgt
     6241 agtctgtaat ctgaactgct gtcgaataga aaaaattcac cgtctgacgc ggataaattg
     6301 caacgatgga aattagccca agagcgcata atagagaggt gagttttttt cgactggtaa
     6361 ttcttagctg cattggtttc atccctgaat gtcagtgcca aaggctgaca ataaccaaag
     6421 cagctatagt acggtgctca ccaccaggcg tggaagtggt gaaccggacc aataccgtga
     6481 ccaacttcca gcgtgtcggc ctgggctaat gctgatgaaa gccagctttt tgcctcctgt
     6541 acggtgtcag cccagtttgt atggcgcggg cgtagtgcag ccaacgccgc agagagcgta
     6601 caaccagtgc cgtgggtgtt tttggtcata atgcgcggtg cggtaaaccg ttgttcaccc
     6661 tcgcgggtaa acagccagtc cgggctttgc tcatcatcca gatgaccacc tttcattagc
     6721 actgcgccac agcccatcgc caacagcgat cgcccttgtt ccagcatttc ctgttcggtg
     6781 cgcgcgtgtg gcgcgtcgag caaggcggca gcttcgggca agtttggcgt tattaatgaa
     6841 acctgtggca ataatcgact gcgcagcgta gcaaccgccg aaggtgaaag cagcgggtcg
     6901 ccgctttttg ccagcataac ggtgtcgagt accacgtttt ggatctgata acgttgcaac
     6961 cgttctgcca ccgcttcaac aatatcggtt tccgccaaca taccgatttt agtggtgtcg
     7021 attcgcacat cgctgaacac cgaatcgagc tgggcggcga caaaatcagg ctcaatgcga
     7081 tacaccgact gtacgccacg ggtattttgc gccaccagtg cagtaataac tgagcaacca
     7141 taagcgccaa gtgccgagaa ggttttaaga tcggcctgaa tccccgcacc accactggga
     7201 tcagtaccgg caatcgtcag agcgttaatt cgtttcatgc ctgcacctcc tgcgtcaatt
     7261 gccagagcgc atcaaggaaa tgtggaacaa aactgcctgg cccctcgctt ctggcgactg
     7321 cgcgctctcc ggcttgtttc atccagtgac aggcagatgc gacattttcc agcgtatcgc
     7381 ctggtaacgc acagcaggca gcgacaaccg ccgataatgc acagccagtt cctaccactt
     7441 tggtcattaa cggatcgcca ccgtgaatgc caacggcgcg atggccatcg gtaacataat
     7501 ccatctcgcc agtgaccacg acgattgcgc cagtttcccg tgccagtgtt tgtgcagcgg
     7561 gtatcgcgtt agctgcggcg tcagtggtat ccactccccg tccaccatta gcaatgccag
     7621 ctaatgccat gatttccgaa gcattaccac gtatggcggt tggcttatga gacagaagtt
     7681 ccagacaaaa acggcggcga taatcgagtg caccaaccgc tactggatca agcgtccagg
     7741 gtgtttgaga gctttttgct tgctcaacgg cggcacacat cgactgtgcg cgtagctgcg
     7801 tcagcgtgcc aacgttaatc agcaacgcac tggcaatagc cgcaaactga ctggcctctt
     7861 cggtttcgat aaccatcgct ggcgatgcac cgagcgccag caaggtattg gcagtaaagg
     7921 tttgcaccac atcgttggtc atgcagtgca caagagggga atgttggtga aaaaggtgta
     7981 acgcgtgcgc agattgcgct gaactcagca ggtcgacttg catagtttgc tcctgccata
     8041 acgtgaagaa gcaatgacct ggtggtccgt gacttcccta cgctggcatt atccagatca
     8101 ggtgatacgg gtatttctca gccttcacgc agaagggcac cccgagtcgt ttggttgcga
     8161 tgataaatcg cagaggagga tggtaatgtc cagcgcacgc gttgtaaacg aggaaaaacg
     8221 gcagttttac aatcgcgtta ataaattatt tgatatatga atccagcacc ttcagaacga
     8281 catccagatc ttcttcacgt tttagctcat ccccctggtg aacgatgtgt tccgtcagat
     8341 gacctttaat cacttcccgc atcagaccgt ttaccgcgcc acggatagca gcaatctgtt
     8401 gtaaaactgc agcgcattcg tgcggttcgt cgagcatttt cttgagcgcc acgacctggc
     8461 cctgaatctt actggcacgc gctttcagtt tctgtttatc acggattgta tgagacatgg
     8521 caacacctgg ttaacaagaa tatgaaaaat catagcacta ttaatctact ggggggtagt
     8581 atcaggtact gggggggagt agaatcagat tgccgaatta atactaagaa ttattatcat
     8641 gaccgaattt acaactcttc ttcagcaagg aaacgcctgg ttcttcatcc ccagcgccat
     8701 cttacttggt gcgcttcatg gcctggaacc agggcactca aaaacgatga tggcggcgtt
     8761 tatcatcgcc atcaaaggca ccattaaaca ggcggtgatg ctcggactgg cagcaactat
     8821 ttcgcatacc gcagtggtct ggttgattgc ctttggcggg atggtggtca gcaagcgctt
     8881 tactgctcaa tcagcagaac cgtggctcca gttgatttcc gcagtgatca ttattagcac
     8941 cgcgttctgg atgttctggc gtacctggca cggcgagcgc aactggctgg agaatatgca
     9001 cgagcatgat catgagcatc atcatcacga tcacgaagat caccacgacc atggacatca
     9061 tcaccatcac gaacatggcg agtatcagga tgctcatgca cgagcccatg ctaatgacat
     9121 taaacgacgc tttgatggta gagaggtcac caactggcaa attttgttat ttggcttaac
     9181 cggtggcctt atcccctgcc cggcagcaat taccgtgctg ttgatatgca ttcagttgaa
     9241 agccctgaca ctgggcgcaa cactggtcgt cagtttcagc attggcctgg cgttaacgct
     9301 tgtcaccgta ggcgttggcg cagcaatcag cgttcagcag gtcgcaaaac gctggagcgg
     9361 atttaacact ctcgctaaac gcgcccccta tttttccagt ctgttgattg gcttagtcgg
     9421 tgtgtatatg ggcgtacatg gcttcatggg cataatgcga taactcaatc ctttaggctt
     9481 gcatgttatc ttcagcccag gatgattctt aaatcagcta tttccgctga ctctctgctc
     9541 gctaaggatg cttttagggc atcctttcat ttacactttt tacgaaatca tgggatcact
     9601 aacaaaatat cgcttgtcag ttatattgta tggcaggaaa gatatgcgac tgatattaca
     9661 gatccccaaa gtggagagtt tatgaccatt aaaaataaga tgttgctggg tgcgcttttg
     9721 ctggttacca gtgccgcctg ggccgcacca gccaccgcgg gttcgaccaa tacctcggga
     9781 atttctaagt atgagttaag cagtttcatt gctgacttta agcatttcaa accaggcgat
     9841 accgtcccag agatgtaccg caccgatgag tacaacatta agcagtggca gttacgtaac
     9901 ctgcccgcgc ctgatgccgg gacgcactgg acctatatgg gtggcgcgta cgtgttgatc
     9961 agcgacaccg acggtaaaat cattaaagcc tacgacggtg agatttttta tcatcgctaa
    10021 aaaaagcccc ctcatcatga gggggaaatg cagacacctt gttatttttt attattagcc
    10081 acttgctcgt cttgcttgtt attagtcgta tttcacgttg attaatgcgg ttgcctccag
    10141 tgcgccagat ttaactttgt ttgtatcgta gacgtagtaa ctggcagtta tcggaattga
    10201 aaccgttgta tctgtaagtt ttgactttgc cgcaatttct tttaaatttg tgtatcgttt
    10261 actgctgtta gtgatattta aaagtgtgac tgacgaacca ttactttcaa gaataaatcc
    10321 tacgccatta tcttccgcac ctttcagtat tgtgttgctg ttggttaaca agtcagaact
    10381 tgataaatac acctgcattt tccgtgatgt ttgattttgt tgacctaata aatttttaca
    10441 acgcagaata atatcccctt gtttactatt tattgttgct ttgttacctg tggctggtaa
    10501 ttgtgaaacg ggaatactgt ctaaagtaat taataaatcg tccggtttgc aggttgtggt
    10561 tatcgggttg tatgacaatg tcagattaaa ggaatagaca cctgcatttt gtgccaatgc
    10621 atttctgtag ctatttacac atgcatccca tgacttgcca cctaataaac acagtagaaa
    10681 ttgtactgcg gcactcccgc gtgtcgatga tgaagcactg gataatgtcg caactccatt
    10741 aatcatcact gaaccgccgc cccccgcaga aacattcact tttgcattac tggccgcagg
    10801 tgtcaccgtt accgaataac cactggtgaa actggtctga ttatttgata acgtgatgtt
    10861 gtttttattg gtccattcaa aatgtaaatt taatttaatg gtatcgttat aaagtccaac
    10921 aataatatcc gacgggctca taggggtgat agtatttgta ctcacgcatg acgaatcatt
    10981 aaaattatgc tcatatgtcg ccatttgtgt ttgagtaagt gcaaaatccc cctgaacaac
    11041 caaattgtgc tgaacagata actcactata acatgccgca tagctcacag gcataaccat
    11101 catcattact ataaataaca ttatacgaat attcataacc acctgactat tactggcaaa
    11161 tatagtttct actttcatca atctctttac cgaaggtgat tgtgcatgaa agtccttgtt
    11221 gcttatcaat tgctacatta accgatggcg gtacttcatt ggtgcgaata aataactggc
    11281 ttccctgacc gacaacgcca atattatgac catggatatc attgacttca taaccaaaca
    11341 ttaatggttg cccatccgct cttaacgctt ttataaacca gggcttgcgc tgatcggtat
    11401 caaaattaac cagtacaacc gcgccgcgat aagaggcggc aattttccgg ttgccacgta
    11461 attctgcttc gctatcgctt tgcgacacat ccagcatcag gtgattttcc cgataaggtg
    11521 tcattccgtc gtataccacc actccattac ggtttgttgt gcgatatttt tgcccattga
    11581 cataagcatc tttaattcct ggcgcattca tcacagcaaa cgtttcggaa agacggttcg
    11641 ccagattaac gccccccgac caggcgacaa tgccccctga aacactggct ccagcctgtc
    11701 gataagtact cgactgacta taactgccat tcactgtcgc aaccggcgcg ttccaggtca
    11761 aattcgcccc agctgtcgtt tcatttccct gattctgata actcaggttg accccatagt
    11821 taaactgatc ccggcttcca acggttcctg ataatcccgt attatttgag gcaaacccct
    11881 gatcatcaaa cgtcgttgag ttagacatat atatttgccg acgaggcgtc gtaacgtcat
    11941 caccccaatc aaagggaatc gatataaaaa tattaaaacg tttctcttca tgatgattct
    12001 cgtcatacgc ctggcttgcc gcgaggatat agcttatccg ccgccagtta ttggaataac
    12061 tcaactgata atccttactg ctgccgctac gcccccagta atctcgccat aacgtactta
    12121 aggacacaga cccccagcct tctggcaatg actggctcat attggcagag aagctatttt
    12181 tacggccaaa atcgttctgg taataatcgg caatgtcata gacatcgttt tcatcacggc
    12241 gataattatc tttattgttt gcccaaacgt gatcattaaa tgtccggtaa tcacgcgatg
    12301 aataacgcca ggccgccaga ccaaaacgcg tcgacgtttg gctcacaaat ttgttgtagg
    12361 caatttgata actttgcccg tcaaacacat cgccgttgtc ttgtttacta tgcgacttcg
    12421 tggcatcgac ggaaatggca ccaatgcgtg tattccagcc agtccccaaa gtaaacgcgt
    12481 aataattatt cgcgaccatc gagccaccat acagcgtcaa taaattatta aaaccatact
    12541 gataacccgc ctggacaaaa tcactttgtt tgctcgcccc ttcaatatgg ctacgacccg
    12601 ccgcaaaatc atatttcgat acgccgggtt gcagcatatt tggcactgct gcataaggca
    12661 ccagataggt ggttaccgag ccgtccgcct ctttcacgct gacatcaaga tctgctccac
    12721 caccggccaa ctgcaaatct gtaatcgcga acgggccagg aggaacctct ttctgataaa
    12781 ccacaaaacc gttctgttca atagttacca gcgcattact ctgagcaatc ccctgcaccc
    12841 gtggcgtaaa attttgtttc gagttaggca acatctgcat atcacgaaac aaccgcacac
    12901 cgctgaagcg aacagaatca aaaatatcgc ttgatgtgta catatcaccc acgcgaagcg
    12961 tgccgagaag ttgggcaaat ccacgttcca gatacagggt attgcttttc cacacccctg
    13021 gattgttatt tgttttactg aaactggcat cagaatgcag ttgccacccc agtaaattta
    13081 acccgctgtt aaaacgtaca tatgtactct tactattacc cgacgcttta tagtcactgt
    13141 aatactgact cacataataa gaggtataaa acgcattaat accccgctcc cagttttccg
    13201 gtggaacata gccactttcc agttcttcca cccaggcctg cgggacactg aaatcgagac
    13261 gaaaaacccc gatatcccag gtatagctcc caccctgaac aagttgctca aatgttaaac
    13321 attgcttacc gctggcgaag ttatcgctat taatgcctaa ccgcttgata acttctattg
    13381 ataaacatgt ttcttgcggg ttgtctttaa caataatctc atatttcccg cgccattgct
    13441 tattgacata aatatcgatg tcatactgcc ccggtaaggg ttgattatca tcaagacgaa
    13501 tatttgcaac ctgctggtct ttcattccac ctatcataaa atgggtatca aaggtttctt
    13561 cagctgcata agcttcaatg ccgagcaata acgctacgat tgctgatgca agtggggtca
    13621 ttctcaacat aggcagctcc tgcaattaaa ttttgtcact aatatagttg ccgtggtcat
    13681 cgataatggt cagataccag ttatttgcat tattactttt gacattaaca ctctgacttt
    13741 ctaagggggc aatcataata gtttcataat tgactttaac attattagct ttgacatccg
    13801 aaatcgtcac ccaattagct gagtcatttt ttatcaccaa tccattgcca ctgcgattta
    13861 ccagtaactt tttaaaggtc gctttattta ccggagcaat acccgctggc cggtaaaaca
    13921 acttaattct gttttgcatc gcaaacttca gtgcattttt gccttcttgc tctggactat
    13981 ttggtggaat atccagaaca ttcagataaa aaatgctttc tttattagtg ggcagtttat
    14041 tcggcataat tttgattttt acttgctgcc cggaatttgc ccctattttt gccactggtg
    14101 gcgttaacat gaaaggaacc tgaatttttt ctggtggtaa cgacgtatcg ccatcatcaa
    14161 tccacgcctg cagcagcgaa gaacggtttc cctggttcat caactgcacc ataacttctt
    14221 tattttctgc cgggtaaata atgcgcgtcc cgtagataac aattccggca tgtgcaggaa
    14281 gg
//
LOCUS       DAZGLJ010000117        14254 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000117 DAZGLJ010000000
VERSION     DAZGLJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.117"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(238..1467)
                     /locus_tag="ACHK49_003030"
     CDS             complement(238..1467)
                     /locus_tag="ACHK49_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9263645.1"
                     /translation="MSRAMNKLSDTLLRKLHGKPAEKNTFYSDGGNLSVKYLTSGKLT
                     WYFTYRAGTGRETRPERIKLGSYPDLSLKAAREKAAQCRTWLAEGKNPRHEMNYTVQE
                     ALKPVTVGDALTYWLEYYVKENRVDYIALKRRLNNHVIQQIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFVLQACKQALKFCRRRRYAISNVLDDLNVADVGKKPDISERVLSNK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGARTVELRLSEIGEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNVLDMWMERLGI
                     LAGTHENVTTLPVARRK"
     gene            1455..1637
                     /locus_tag="ACHK49_003031"
     CDS             1455..1637
                     /locus_tag="ACHK49_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263646.1"
                     /translation="MLCSRFTPSLRKSDYTSDYTDRCTKGYNVKQAGTRKITNLLIIK
                     NIMHSLKHYETAKNTK"
     gene            1644..2546
                     /gene="tqsA"
                     /locus_tag="ACHK49_003032"
                     /pseudo
     CDS             1644..2546
                     /gene="tqsA"
                     /locus_tag="ACHK49_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            complement(2574..3962)
                     /gene="pntB"
                     /locus_tag="ACHK49_003033"
     CDS             complement(2574..3962)
                     /gene="pntB"
                     /locus_tag="ACHK49_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO9263647.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(3973..5505)
                     /gene="pntA"
                     /locus_tag="ACHK49_003034"
     CDS             complement(3973..5505)
                     /gene="pntA"
                     /locus_tag="ACHK49_003034"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO9263648.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            6029..6973
                     /gene="ydgH"
                     /locus_tag="ACHK49_003035"
     CDS             6029..6973
                     /gene="ydgH"
                     /locus_tag="ACHK49_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO9263649.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            7159..8541
                     /gene="ydgI"
                     /locus_tag="ACHK49_003036"
     CDS             7159..8541
                     /gene="ydgI"
                     /locus_tag="ACHK49_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9263650.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            8578..9300
                     /gene="folM"
                     /locus_tag="ACHK49_003037"
     CDS             8578..9300
                     /gene="folM"
                     /locus_tag="ACHK49_003037"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO9263651.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(9297..9632)
                     /gene="ydgC"
                     /locus_tag="ACHK49_003038"
     CDS             complement(9297..9632)
                     /gene="ydgC"
                     /locus_tag="ACHK49_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO9263652.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            9761..10480
                     /gene="rstA"
                     /locus_tag="ACHK49_003039"
     CDS             9761..10480
                     /gene="rstA"
                     /locus_tag="ACHK49_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO9263653.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            10484..11785
                     /gene="rstB"
                     /locus_tag="ACHK49_003040"
     CDS             10484..11785
                     /gene="rstB"
                     /locus_tag="ACHK49_003040"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO9263654.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            11861..12790
                     /gene="tus"
                     /locus_tag="ACHK49_003041"
     CDS             11861..12790
                     /gene="tus"
                     /locus_tag="ACHK49_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO9263655.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12787..14190)
                     /gene="fumC"
                     /locus_tag="ACHK49_003042"
     CDS             complement(12787..14190)
                     /gene="fumC"
                     /locus_tag="ACHK49_003042"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO9263656.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAVSLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
BASE COUNT         3411 a         3740 c         3445 g         3658 t
ORIGIN      
        1 tcatttaaat tcatgttaat acaaaggcat aaaatattgt ttcatgcctt ttttctcgct
       61 atttaaagag tgattcgcta gtgtataaaa aaccagtaac acattaaaaa tcagctactt
      121 ataaatctgt acactttttc gcctcttgtt cgtagttgtt ccgcattgtt gctcattgtt
      181 gcacgttgta tctgttcgca tatccagtat gcgcatactg aaaaaatacg aaaaaaatta
      241 ttttcttctg gctactggta gcgtggttac gttttcatgt gttcccgcca gtatccctaa
      301 ccgctccatc cacatatcca gcacattgcg tttagcatcc agataacggg aatgattata
      361 aactcgctgc attcctggca tctggtggcc tgtaagctgc tccacgacat gcggatcaac
      421 gcctaaatcg ttcagcatgg ttgtaaaggt gcgccggatg tcatgcagtg accagtgagg
      481 atgattaagc ctcctgtgcg ctaatcttcc atactgcgat acgctggcct cctgtttcac
      541 ttcccccagc aataagcccg tgtgcctgtt ctgctccacc agctgcgtga cgaacggcag
      601 gatcgcttcc ggtatgggcc ggaatattgc gaccttcgtt ttgctgtgct ccttcggaac
      661 ggtccatagc atttccgtaa aatcccactc cccgatctcc gatagcctca gttctaccgt
      721 cctggctccg aagacaatca ggaggcggat taacgcgacg tagtaagggg aaaatatttt
      781 tttgtccagt gcctgcaata attcgccaag ttctttgtta cttaagacac gttcgcttat
      841 atccggtttt ttcccaacgt ccgccacgtt cagatcgtcc agaacgttgc tgattgcata
      901 gcgccgccta cggcagaact taagcgcctg tttgcacgcc tgtagcacga atccggcagt
      961 aacaggcgtt cgctttgcca cctggtcaaa acaggccagc cagtgccgta gctcgcattt
     1021 atccagcggc atagcaccaa tctgctgtat tacgtgatta ttaagtcgcc ttttcagggc
     1081 gatataatcc acgcggtttt cctttacgta atactcaagc cagtaggtga gcgcatcgcc
     1141 aaccgttacg ggctttaacg cttcctgtac ggtgtaattc atctcatgac gtggattttt
     1201 cccctcagcc agccatgtgc gacactgtgc ggctttttcc ctggctgctt tcaggctcag
     1261 atcaggataa cttcccagct taatgcgttc gggtcgtgtc tcccttcccg ttccggccct
     1321 gtatgtgaaa taccaggtca atttccctga tgttaaatat ttcacgctca ggtttccgcc
     1381 atcactataa aacgtgtttt tctccgctgg cttaccatga agtttcctta gcaaggtatc
     1441 gctcagtttg ttcattgctc tgctcacgtt ttaccccctc tttacgaaaa agtgactaca
     1501 ccagtgacta caccgatcgt tgcacaaagg gctacaacgt gaaacaagct ggaacaagga
     1561 aaatcacaaa tctgttgata atcaaaaaca taatgcacag cctgaaacat tatgaaacag
     1621 caaaaaacac taaatgataa aatgctgtta ttcttaaccc gctggtgcaa cacatggtcc
     1681 gctggcgagt gccgcgtgta ctggcggtgt cgattttgat gaccatcatc gtgatggcga
     1741 tggtgttgct attagcttat ctgggttccg cgctcaacga gttgacgcgg acgttaccgc
     1801 aatatcgcaa ctctattatg acgccgctgc aagctcttga gccgttgttg caacgcgtag
     1861 ggattgacgt ctcagttgac cagctggcgc attacattga tccgaacgcg gcgatgacgt
     1921 tgctcaccaa cttattgacg cagttatcta atgccatgtc atcaatattt ttattgctgc
     1981 tgacggtgct gtttatgctg ctcgaagtgc cacaattgcc cggaaaattt cagcaaatga
     2041 tggcgcgtcc ggttgaaggg atggcggcga ttcaacgtgc aattgacagt gtttctcatt
     2101 atctggtgct gaaaacagcc atcagcatca tcaccggcct ggtcgcctgg gcgatgctcg
     2161 ccgcactcga tgttcgcttc gcttttgtct ggggattgct ggcctttgcg cttaattaca
     2221 tccctaatat tggttcagtt ctcgcggcaa tcccccctat cgctcaggta ctggtgttta
     2281 atggcttcta cgaagcgttg ctggtgctgg cgggatatct gctgattaat ctggtcttcg
     2341 gcaatattct ggagccgcgc atcatgggac gtgggctggg gctttccaca ttggtggtat
     2401 ttttgtcgtt gattttttgg ggatggttgt taggaccggt gggtatgctg ctttccgtgc
     2461 cgttaacaat tattgtcaaa attgcgcttg aacaaacagc gggaggtcaa agcatcgccg
     2521 ttctgttaag cgatctcaat aaagagtggc ggcctccgca gaggccgtca gggttacaga
     2581 gctttcagga ttgcatccac gctggctttg gcgtcaccaa acagcatgtg ggtgttttcc
     2641 ttgaagaaca gcgggttttg cacaccagca tagccagtgt tcatcgaacg tttaaagaca
     2701 atcacgttct gcgctttcca cacttccagc acaggcatac cagcaatagg actcttcgga
     2761 tcatcctgcg ccgccgggtt aaccgtatcg ttagcaccaa tcaccagtac ggtatcggta
     2821 tcagcaaagt catcgttgat ctcgtccatt tccagcacga tgtcatacgg tacttttgct
     2881 tcagccagca atacgttcat atgtccaggc aaacgccccg caaccgggtg gataccgaaa
     2941 cgcacgttga taccacgagc gcgcagtttc tcggtaattt cagcgacagg atattgcgcc
     3001 tgcgcgactg ccatgccgta ccccggagta atgatcactg aatgggagtt tttcagcagt
     3061 tccgctgtct cttctgcggt gatttcgcgg tgctcgccca cttcctgatc atcgccagta
     3121 gaagagccgt cggtgccgaa accacccgca ataacgctga taaaggaacg gttcatcgcc
     3181 ttacacataa tgtaagaaag gatagcgccc gaagaaccga ccagcgcacc ggtcacaatc
     3241 agcaggtcgt tgctgagcat aaagcccgca gccgcagccg cccagccgga gtacgagttc
     3301 agcatcgaca ccaccactgg catatctgca ccaccgatgg aggcgactaa atgccagccg
     3361 aataccagcg caatggcggt cattatcagc aatgccagca cttgcaggcc gacgctatcc
     3421 gtgcgaacaa atacaatcag cagcaggaag gaaacgacca gagccgccag gttcattttg
     3481 tgacggtttg gcagcatcaa cggtttagac gaaatcttgc cacacagttt gccgaacgcc
     3541 accaccgaac ccgtgaacgt taccgccccg atgaagatac cgaggaacac ttccgtcagg
     3601 tgaatattga ccagaatcgg tgccattccc gcgtcatgat gcagataact gttaaagcca
     3661 accagcactg ccgccagacc cacgaagcta tgcaggatcg ccaccagttc tggcatttcg
     3721 gtcatttcaa ctttcttcgc cagacggata ccaattgccc caccaatgac catcgccagc
     3781 aagatccagc caacattacc cgtatccggt ccaaaaatgg ttgcgattaa cgcaatcgcc
     3841 atcccggcga taccgaagtt gttaccctgg cgagacgttt catgtttcga aagaccggcc
     3901 agactgaaga taaacaggat cgcggcaaca atgtatgcag ctgtaactaa tcctccagac
     3961 atatgttacc ccttaatttt tgcggaacat tttcagcatg cgctgagtca cggtgaagcc
     4021 accgaaaata ttaatgctgg ctataagcac cgcgataaaa ctaaggaagc taacccagcc
     4081 gccctggcca atctgcaaca gtgctccgac aacaataatc cctgaaatcg cgttggtgac
     4141 cgacatcaac ggtgtatgca gcgcatgcga tacattccac accacgtaat aaccgacaac
     4201 gcaggccagc gcgaaaacgg tgaagtgccc aagaaactct ttcggcgcaa cgcttgccag
     4261 ccaaccgaaa aggatgattg ccagcgccat caatgcgtat ttacgccacg gtgagcaggc
     4321 acatttttcc tcagttttca cttccggtgc cgctttttgt gccgcctgcg gctgagctga
     4381 tacctgaatc ggcggtgccg gccaggtaat ttcgcccgca cggatcacgg tcacgccgcg
     4441 aatcaccaca tcatcaaaat caacagtgat attgccgtct ttctctttgc acaacagttt
     4501 cagcagatta acgaggttag taccgtaaag ctgtgaggat tgcgtcggca gacggcccgg
     4561 aagatcggta taaccaatca ctttgacacc attttccgta gtgaagattt cacccggcac
     4621 ggtgtattca cagttgccac cgttttgggc tgccaggtcg acaatcacac tgcccgcctt
     4681 catggagtca accatttcac gggtaattag cttcggcgct ggtttgcctg gaataagcgc
     4741 ggtggtgaca atgatatcga cctcttttgc ctgggcggca aagagttcca tttccgcttt
     4801 gatgaacgcg tccgacatca ctttggcata gccatcgccg ctgcccgctt cctctttaaa
     4861 atccagctcg aggaattccg cgcccatact ttgaacttgt tctttcactt ccgggcgggt
     4921 gtcgaatgca cgcacaatcg cgccgagact gtttgctgcg ccaatggcgg ccagacctgc
     4981 aacacccgca ccaatcacca tcacttttgc cggtggcact ttcccggccg cagtaatttg
     5041 cccggtaaag aagcgcccaa attcatgtgc cgcttcaaca atggcgcgat aaccggcgat
     5101 gttcgccatc gagcttagtg cgtccagcga ttgtgcgcgt gagatacgcg gcacagaatc
     5161 catcgccatc acggtcacgt tacgttccgc aagtttttgc attaattccg gattctgcgc
     5221 aggccagata aaactcacca gcgttgtccc aggattcagt aacgcaattt catcatctaa
     5281 cggcgcattg accttcagaa tgatctctga ctgccagacg ctattccctt ctacaatttc
     5341 agcgcccgct tgcacaaacg ctttatcgtc aaaacttgcc agttgacccg cgccgctctc
     5401 taccgcgacg gtaaaaccca gtttcagcag ctgttccact gtttttggcg ttgctgcaac
     5461 acgggtttca ttggttaacc gttctcttgg tatgccaatt cgcatgatat tcccttccat
     5521 cggttttatt gatgatggtt tgcctgtgtc aggagccaca caagccgctc atgtacgagc
     5581 taaatgttac tccgttaaaa taaattagta acaaacgccc tataacgtac tgaaaattat
     5641 gcctgtgatc tagcgccaaa aatcagtatt tcggcgtgaa ctcgcaaaat attaacgatt
     5701 cagccgtgat agtgggataa acaccttaga acgccggata aagactgata attgtcttcg
     5761 acggtcgggt aaaacgagac atcgccccgg cacgaatcac tacttaacat taaattaact
     5821 tatacaattc agttgcttca gtagtaatga tgctgatacg gctgtttttt aagcatagac
     5881 ggtcatttga gcaggattaa aattggctta aggaatgtga tatgaaaaat gacgcagaca
     5941 gttacaccgt ttaaatgcaa taatcagcca cgtttctcgt taataacaat accagtacct
     6001 ggtttgcgca aggcgaagga ttatttttat gaagcttaag aacaccctcc tggcgtcggc
     6061 actgctttct gctacggcat tctccgttaa cgcagcaaca gaactgacac cggagcaagc
     6121 ggcagcggtt aaaccttttg accgtgtagt ggttaccggt cgttttaatg ctattggcga
     6181 agcggtgaaa gccgtttctc gtcgcgcaga taaagaaggt gccgcctctt tttatgttgt
     6241 cgacacttct gattttggta acagcggtaa ctggcgtgtg gtcgctgacc tctataaagc
     6301 cgatgctgaa aaagcagaag aaacaagtaa tcgcgtaatt aacggtgttg tcgaactgcc
     6361 gaaagatcag gctgttctga ttgaaccgtt tgacacggtc accgtccagg gcttctatcg
     6421 tagccagcca gaagtcaatg atgccatcac caaagcagca aaagcgaaag gtgcctactc
     6481 tttctacatt gttcgtcaaa tcgatgccaa ccagggcggc aaccagcgta ttactgcatt
     6541 catctataaa aaagatgcta agaaacgtat cgtccagagc ccggatgtga tcccggcaga
     6601 ttccgaagca ggacgtgcag ctctggctgc cggtggcgaa gccgcgaaga aagttgagat
     6661 cccaggtgtt gcgactaccg catcaccaag ttctgaagtc ggtcgtttct ttgaaaccca
     6721 gtcatcaaaa ggcggacgtt acactgtcac gcttcctgac ggcactaaag tcgaagaact
     6781 gaacaaagcg acagcagcga tgatggtccc gttcgacagc atcaaattct ctggcaacta
     6841 cggcaacatg accgaagtct cctatcaggt tgcgaaacgt gccgcgaaga aaggtgctaa
     6901 gtattaccac atcacccgcc agtggcagga acgtggtaat aacctgaccg tcagcgcaga
     6961 tctgtataaa taactattca gttactccta taaggcggct tgatgccgcc tttcgcattt
     7021 ttagtcacaa atttccccgc gaccattgca tgcagacaca tctcttcgta aaatcccgcg
     7081 cttcagtggc aaactccatt tttatgcgtt ttgccctaat atttattctt cttatcacgt
     7141 tttaatcact ggatatcgat ggaaaagaaa ctgggactga gcgcactcac cgcgctggta
     7201 ttaagctcaa tgctgggcgc gggtgttttc agtctgccgc aaaatatggc ggcagttgcc
     7261 agcccggcag cactgctcat cggctggggt attactggcg ctggcatttt attgctggcc
     7321 tttgccatgc tgatcctcac gcgcattcgt cccgaacttg acggcggtat ctttacctat
     7381 gcccgcgaag ggtttggcga gctaatcggc ttttgttccg catggggata ctggctgtgc
     7441 gcagtcatcg ccaacgtctc ctatctggtg atcgtttttt ccgcgttaag cttttttacg
     7501 gacacgccgg aattgcgcct gttcggcgat ggtaatacct ggcaatcgat agtcggtgca
     7561 tcggctttat tgtggattgt tcactttttg atcctgcgcg gggtgcaaac cgctgccagc
     7621 attaacctgg tggcgacact ggcaaaattg ttgccgctgg gtctgtttgt tgtgctggcg
     7681 atgatgatgt tcaaactgga taccttcaag ctcgacttca ccggacttgc acttggcgta
     7741 cccgtgtggg aacaggtgaa aaacaccatg ctgatcaccc tgtgggtttt cattggtgtg
     7801 gaaggtgccg tggttgtttc tgcgcgtgcg cgtaataaac gtgatgttgg caaagcgaca
     7861 ctgctggcgg ttctctccgc tctgggcgtt tacttgctgg taacgctgct ttcgctgggc
     7921 gtggtggcac gtcctgaact ggctgaaatt cgtaacccgt caatggccgg tctgatggtg
     7981 gaaatgatgg gaccatgggg cgaaatcatc atcgctgccg gtttgatcgt ttccgtttgc
     8041 ggcgcgtact taagctggac catcatggcg gcagaagttc cgttcctggc tgccactcat
     8101 aaagcattcc cacgcatttt tgcgcgccag aacgcacaag ctgcgccatc cgcctcgctg
     8161 tggttgacca acatctgcgt gcaaatttgt ctggtactca tctggctgac cggttccgat
     8221 tacaacacgt tgctgaccat cgcctcagaa atgattctgg tgccctattt cctggtcggc
     8281 gcgttcttgc tgaaaatcgc aacccgtcca ctgcataaag cggtaggtgt cggtgcctgc
     8341 atttatggct tatggttatt gtatgcctct ggtccaatgc acttgctgtt atccgttgtt
     8401 ctgtatgcgc cggggctgct ggttttcctg tatgcgcgca aaacgcatac acatgataat
     8461 gtactgaacc gtcaggaaat ggtgctgatt ggcatgctgt taattgcctc agttccggcg
     8521 acctggatgc tggtgggata acgttcatac catcgttgga tagcaaagga gataacgatg
     8581 ggtaaagccc agcccttgcc aatattaatt actggcggag gtcgtcgcat cggcctcgcc
     8641 ctcgcctggc atttcattaa tcaaaagcaa ccggtgattg tcagctatcg gacgcactat
     8701 ccagccattg atggactgat taaggcaggt gcgcagtgta ttcaggctga tttttcgacc
     8761 aacgacggtg tgatggcgtt tgccgatgaa gtactaaaaa gcacccatgg tctgcgtgct
     8821 attttgcata acgccagtgc gtggatggcg gaaaaaccag gtacaccact ggccgacgta
     8881 ctggcttgca tgatgcagat ccacgttaat accccatacc tgctcaacca tgcgctggaa
     8941 agattactgc gtgggcacgg acacgccgcc agcgatatca ttcactttac cgattatgtg
     9001 gtggagcgcg gtagcgacaa acatgttgcg tatgctgcaa gcaaagcggc actggataat
     9061 atgacccgct cgtttgcccg caagctggca ccggaagtga aagtgaattc tattgcgcca
     9121 tcgctgatcc tgtttaatga acatgatgat gccgaatatc gacaacaggc gctgaataaa
     9181 tcactgatga aaaccgcgcc tggcgagaaa gaagtgatcg acctggtcga ttacttactt
     9241 accagctgct ttgtcaccgg acgcagtttc ccacttgatg gcggtcgtca tctgcgttaa
     9301 tgcagcttta tccagcatat aatcaacacc catgcgctta tcccccaaca cgcaacagaa
     9361 ccaacaaaag cggcgggcaa gcgcatcatt ccggtgaaat accacagcga caccaggtaa
     9421 acaaaatagg gaataatcga ccacatacta aaaatgatgg ttgcgcgtaa ggcttcaatg
     9481 ccgcgttcgc tggcaacaat ataatgcgcg ataagcgcaa aggtcgggaa aagtggaatc
     9541 agcccggcga tataataatt tttcgttttt gctaaaacac caatcaacag cactaccagc
     9601 gcaccgagcg cggctttgat taccagcccc atctttttac cttaacactt ccataacaag
     9661 tcatcagtag aatacctgat gaaaacttgt ttagaaacga ttgatagtaa gtaaaaacag
     9721 cgcggtgtat tgtgacgttt ttatatctac cgtgaatgtt atgaacacta tcgtatttgt
     9781 ggaagatgat gcggaagtcg gttcactgat tgccgcgtac ctggcaaaac atgatatgca
     9841 ggttaccgta gaaccgcgcg gcgaccaggc cgaagaaacc attttgcgag aaagtccgga
     9901 tttggtgtta ctcgacatca tgctaccagg caaggacggc atgaccattt gtcgtgattt
     9961 acgcgcaaag tggtctggac cgattgttct tctaacctct ctcgatagcg atatgaacca
    10021 catcctggca ctggaaatgg gtgcctgcga ctatattctc aaaacgacgc cccctgctgt
    10081 tttgctagcg cgtttacgtt tgcatttgcg tcagaatgag caagccacac tgaccaaagg
    10141 tcttcaggaa acgtctctga ctccctacaa agccctgcat ttcggcacgt tgaccatcga
    10201 tcccatcaac cgcgtagtca ccctggctaa cactgaaatc tcgctctcga cagctgattt
    10261 cgaattattg tgggaattag ctacccatgc cgggcaaatc atggaccgcg atgcattgct
    10321 gaaaaattta cgcggcgtca gttatgacgg actggatcgt agcgtggacg tggctatttc
    10381 gcggttaaga aaaaaactgc tcgataacgc cgcagaacct tatcgcatta aaactgtgcg
    10441 taacaaaggc tatctttttg cgcctcatgc atgggaataa gcgatgaaaa aactgtttat
    10501 ccagttttac ctgttattgt ttgtctgctt ccttgtgatg tctctgctgg ttgggttggt
    10561 gtacaaattt accgccgaac gcgcgggcaa acagtcgctg gatgatttga tgaacagttc
    10621 gctgtatctg atgcgcagcg aattgcgtga gatcccccca cacgactggg gtaagacgct
    10681 gaaagagatg gatttaaatc tctctttcga tctgcgtgtc gagccactga gtaaatacca
    10741 tcttgatgat atttccatgc accgactgcg tggcggcgaa attgtcgccc tggacgatca
    10801 gtacacgttt ttgcagcgta tcccgcgcag ccactacgtg ctggcagttg gtcctgttcc
    10861 ttatctttat tatctccatc agatgcgatt gctggatatc gccctgatcg cttttattgc
    10921 tatttccctc gcctttccgg tgtttatctg gatgcgtccg cactggcagg atatgttaaa
    10981 actggaagca gcggcgcaac gatttggcga tgggcatctc aatgagcgta tccactttga
    11041 tgagggttcg agctttgaac gacttggtat cgcatttaac cagatggcgg acaatatcaa
    11101 cgccttgatt gccagcaaaa aacagcttat tgacggtatc gctcacgaac tgcgaacacc
    11161 gttagtgcgc ctgcgttatc gactggagat gagcgataac ctgagcgccg ccgaatccca
    11221 ggcgttgaat cgtgatatca gtcaacttga agctttaatt gaagagctgc tgacttatgc
    11281 ccgactcgat cgcccacaaa acgagcttca tcttagcgaa ccagacctgc cgttgtggct
    11341 gtcaacgcat ctggcagata ttcaggcagt aacgcccgat aaaacggtac ggataaaaac
    11401 gctcgtgcaa ggccattatg cggcgttgga tatgcgctta atggagcgcg tgctggataa
    11461 tttgctcaat aacgccctgc ggtactgcca ttcaacggtt gaaaccagcc tgctactgtc
    11521 ggggaataga gcgacattaa ttgttgagga tgatggccca gggattgccc cagaaaaccg
    11581 cgaacatatc tttgaacctt ttgttcgcct cgatcccagc cgggatcgct caaccggcgg
    11641 ctgcgggctg gggctggcaa ttgtccactc tatagcactg gcaatgggcg gtacggttaa
    11701 ttgtgacacc agcgaactgg gtggtgctcg cttctcgttt agctggccgt tatggcataa
    11761 catcccgcaa tttacctctg cctgacacta cgcgcacgat ggtcaagtca ccacgactgt
    11821 gctataaaat aagtatgttg taactaaagt ggttaatatt atggcgcgtt acgatctcgt
    11881 agaccgactc aacactacct ttcgccagat ggaacaagag ctggctacat ttgccgctca
    11941 tcttgagcaa cacaagctat tggttgcccg cgtgttctct ttgccggagg taaaaaaaga
    12001 ggatgagcat aatccgctta atcgtattga ggtaaaacaa catctcggca acgacgcgca
    12061 gtcgctggcg ttgcgtcatt tccgccattt atttattcaa caacagtccg aaaatcgcag
    12121 cagcaaggcc gctgtccgtc tgcctggcgt gttgtgttac caggtcgata acctttcgca
    12181 agcagcgttg gtcagtcata ttcagcacat caataaactc aagaccacgt tcgagcatat
    12241 cgtcacggtt gaatcagaac tccccaccgc ggcacgtttt gaatgggtgc atcgtcattt
    12301 gccggggctg atcaccctta atgcttaccg cacgctcacc gttctgcacg accccgccac
    12361 tttacgcttt ggttgggcta ataaacatat cattaagaat ttgcatcgtg atgaagtcct
    12421 ggcacagctg gaaaaaagcc tgaaatcacc acgcagtgtc gcaccgtgga cgcgcgagga
    12481 gtggcaaaga aaactggagc gagagtatca ggatatcgct gccctgccac agaacgcgaa
    12541 gttaaaaatc aaacgtccgg tgaaggtgca gccgattgcc cgcgtctggt acaaaggaga
    12601 tcaaaaacaa gtccaacacg cctgccctac accactgatt gcactgatta atcgggataa
    12661 tggcgcgggc gtgccggacg ttggtgagtt gttaaattac gatgccgaca atgtgcagca
    12721 ccgttataaa ccacaggcgc agccgcttcg tttgatcatt ccacggctgc acctgtatgt
    12781 tgcagattaa cgcccggctt tcatactgcc gaccatctgt tctggccgta cccagctgtc
    12841 aaactcggct tcgctaagat accccagcgc aagggccgca gcttttaagg tcagcccttc
    12901 tttatgcgct tttttggcga tctccgcggc tttgtcataa ccaatgtggg tgttaagcgc
    12961 agtcaccagc atcagcgatt cattgagtaa ttgattgatt cgctcacgat tcggttcaat
    13021 acccactgcg cagtgtttgt taaaactttc catgccatct gccagcaagc gcaccgattg
    13081 caggaaattg tggatcacca ttggacggaa gacgttcagt tcaaagttac cggaagcgcc
    13141 ccccatgttg atcgccacgt cgttccccat cacctgacag cagagcatgg ttaatgcctc
    13201 acactgtgtt ggattcactt tccccggcat gattgagctg cccggctcat tttccgggat
    13261 tgagatttca ccaattccgc agcgcgggcc agaggccagc cagcggacat cattggcgat
    13321 tttcatcagt gacacagcca accctttcaa cgcgccgtgc gcctgaacca gggcatcaca
    13381 ggtcgccagc gcttcaaatt tgttcggcgc ggtaacaaac ggtgcacagg taatgactgc
    13441 cagttcatct gctacgcgac gcgcatactc cggatgggta tttagtccag tacccaccgc
    13501 tgtaccgcca agagccagtt ccgctacgtg aggcaggctg tattcgatat gtttgagatt
    13561 atgctcgagc atcgctaccc agccggaaat ctcctgccct agtgttagcg gcgtggcgtc
    13621 ctgcaagtgg gttcgaccga ttttgacgat atcggcaaat gcacgcgatt tttcactcag
    13681 tgtctgtgtc agggttttaa gctgcggaat gagttgcttg cgcagcgcca gcagcgccgc
    13741 aacgtgcatc gccgtcggaa agacatcgtt ggaactttgg cttttgttca cgtcgtcgtt
    13801 agggtgaact ttacgttcca tcccgcgcac gccgccgagt aattcactgg cccggttagc
    13861 cagcacttcg ttcatgttca tgttactttg cgtgccggag ccggtctgcc agatagccag
    13921 cgggaattcg tcgtcatgct gtcctgccag tacttcatcc gccgcctgcc gaatggcgct
    13981 cgctttctct tcagacaaca agcctaaatc ttcattaact tttgccgctg cgcgcttggt
    14041 tagcgccagc gcatgaatca gtgaggtggg cattttctcc gtcgaaatgc ggaaatgctc
    14101 cagcgagcgt tgagtttgtg cgccccacag cttatctgcc gggacatcaa tcgcccccat
    14161 cgaatctttt tcgctgcgta ctgtattcat ggcctgctcc tcacctgttt aattttttct
    14221 ttctgttttg ctttcgttaa gcaactttta gctc
//
LOCUS       DAZGLJ010000118        14224 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000118 DAZGLJ010000000
VERSION     DAZGLJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.118"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            16..582
                     /locus_tag="ACHK49_003043"
     CDS             16..582
                     /locus_tag="ACHK49_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9263657.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            633..3092
                     /gene="ybgQ"
                     /locus_tag="ACHK49_003044"
     CDS             633..3092
                     /gene="ybgQ"
                     /locus_tag="ACHK49_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO9263658.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNDVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3107..3838
                     /gene="ybgP"
                     /locus_tag="ACHK49_003045"
     CDS             3107..3838
                     /gene="ybgP"
                     /locus_tag="ACHK49_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9263659.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNIGNYSGSQFYLGYMD
                     DYGALRMTTLNYSGQCHLQAVEAKK"
     gene            3835..4896
                     /gene="ybgO"
                     /locus_tag="ACHK49_003046"
     CDS             3835..4896
                     /gene="ybgO"
                     /locus_tag="ACHK49_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9263660.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5048..6094
                     /gene="abrB"
                     /locus_tag="ACHK49_003047"
     CDS             5048..6094
                     /gene="abrB"
                     /locus_tag="ACHK49_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO9263661.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6091..6882)
                     /gene="nei"
                     /locus_tag="ACHK49_003048"
     CDS             complement(6091..6882)
                     /gene="nei"
                     /locus_tag="ACHK49_003048"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO9263662.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNELTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERVLSPRFRNRQFAGLLLE
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6918..7652)
                     /gene="pxpA"
                     /locus_tag="ACHK49_003049"
     CDS             complement(6918..7652)
                     /gene="pxpA"
                     /locus_tag="ACHK49_003049"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO9263663.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEQQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7642..8574)
                     /gene="pxpC"
                     /locus_tag="ACHK49_003050"
     CDS             complement(7642..8574)
                     /gene="pxpC"
                     /locus_tag="ACHK49_003050"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO9263664.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGSGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8568..9224)
                     /gene="pxpB"
                     /locus_tag="ACHK49_003051"
     CDS             complement(8568..9224)
                     /gene="pxpB"
                     /locus_tag="ACHK49_003051"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO9263665.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9247..9990)
                     /gene="ybgI"
                     /locus_tag="ACHK49_003052"
     CDS             complement(9247..9990)
                     /gene="ybgI"
                     /locus_tag="ACHK49_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO9263666.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10261..11742
                     /gene="dtpD"
                     /locus_tag="ACHK49_003053"
     CDS             10261..11742
                     /gene="dtpD"
                     /locus_tag="ACHK49_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO9263667.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11784..13202)
                     /gene="phrB"
                     /locus_tag="ACHK49_003054"
     CDS             complement(11784..13202)
                     /gene="phrB"
                     /locus_tag="ACHK49_003054"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO9263668.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVKTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMISASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13199..13708)
                     /gene="ybgA"
                     /locus_tag="ACHK49_003055"
     CDS             complement(13199..13708)
                     /gene="ybgA"
                     /locus_tag="ACHK49_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO9263669.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13859..14224)
                     /locus_tag="ACHK49_003056"
                     /pseudo
     CDS             complement(13859..14224)
                     /locus_tag="ACHK49_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3416 a         3809 c         3466 g         3533 t
ORIGIN      
        1 ttaaaggact ccgttatgtt taaaggacaa aaaacattgg ccgcactggc cgtatctctg
       61 ctgttcacag cacctgttta tgcggctgat gaaggatccg gtgaaattca ctttaaaggt
      121 gaagttattg aagcaccgtg tgaaatacat caggatgata ttgataaaga ggttgaactc
      181 ggtcaggtga ccaccagcca cattaatcag tcacatcaca gcgatgccgt tgctgtcgac
      241 ctgcgcttag tcaactgtga tctggaaaac tccagcaacg gttccggtgg caagatttct
      301 aaagttgcag tcacctttga tagctcagcg aaaaccaccg gcgcagatcc gattctcaac
      361 aacaccagca cgggtgaagc caccggcgtc ggcgtacgtt taatgaataa agatcaaagc
      421 aatatcgttt taggcaccgc tactccagat atcgacctgg ctccgacctc cagcgaacaa
      481 acgctgaatt tctttgcctg gatggaacaa attgatcagg ctacacctgt aacgccaggc
      541 gcagttacgg cgaatgcaac ctacgtgctg gattataaat aagtattact ttggcggcgc
      601 tatcgccgcc tttcataaaa tatttatcag gtatggacac agtgaatatt tatcgactct
      661 cttttgtatc ctgcctggtc gtggcgatgc cttgcgcatt ggcggtcgaa ttcaacctta
      721 atgttctcga taaatcaatg cgcgaccgca ttgatatttc attattaaag gaaaaaggag
      781 tcattgctcc cggtgagtat tttgttagcg ttgcggtaaa taataaccaa atcagtaacg
      841 ggcaaaagat taactggcac aaaaatgacg ataaaaccat tccgtgcatc aatgatttac
      901 tggtcgataa atttggctta aaacctgaag tccgtcagtc gttaccattg ataaatcagt
      961 gcgtcgattt tagctcccga cctgaaatgc tcttcaattt cgatcaagcc aatcagcaac
     1021 taaatatcac cattccgcaa gcctggctgg cgtggcactc agaaaactgg accccaccct
     1081 ccacatggaa agaaggtgtc gccggtgtcc tgatggatta caacttgttt gccagcagct
     1141 accgcccaca ggatggcagc agcagcacta atcttaacgc ctatggaaca gccggaatca
     1201 acacgggggc atggcgctta cgcagtgatt atcagttgaa tcagactgat agcgatgata
     1261 accatgaaca gtcaggcgaa atatcacgta cctatctttt tcgcccgtta ccgcaattag
     1321 gttcaaaatt aaccctcggc gaaacggatt ttagttccaa tattttcgac ggtttttctt
     1381 ataccggcgc ggcactggca agtgacgatc gaatgttgcc atgggaacta cgcggctacg
     1441 ccccacaaat tagcggtatt gcacagacca atgccacggt gacgatcagt caatcaggcc
     1501 gcgtcattta ccagaaaaaa gtcccaccag gcccatttat cattgacgac cttaatcagt
     1561 ctgttcaggg cacactggat gtcaaagtga cggaagaaga tggtcgggtg aacaatttcc
     1621 aggtttcggc agcatcgacg cccttcctga ctcgtcaggg acaggttcgc tataaactgg
     1681 ccgcgggtca gccacggccc tccatgtcac atcaaactga aaatgaaacc ttttttagca
     1741 atgatgtttc ctgggggatg ctgtcaaaca cctcgctgta cggcggcctg ctgctttctg
     1801 gtgatgacta ccattctgcc gcaataggta tcgggcaaaa tatgctgtgg cttggcgcgc
     1861 tgtcgtttga tgtcacgtgg gccagtagtc actttgatac tcagcaggac gagcggggct
     1921 taagctaccg ttttaattac agcaaacaag tggatgccac caacagcacg atttccctcg
     1981 ccgcttatcg tttctccgat cgtcattttc acagctacgc caactatctt gatcacaaat
     2041 acaacgacag cgatgcgcag gacgaaaaac agacgatcag cttatctgtg ggccaaccga
     2101 ttaccccact aaacctcaat ctttacgcca acctgctaca tcaaacctgg tggaatgcag
     2161 acgcctccac gaccgccaac atcacggcag gttttaatgt tgatattggt gactggagag
     2221 atatctctat ttcgacgtca ttcaatacaa cccattacga agataaagat cgcgacaacc
     2281 agatttacct gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc tatgacatgc
     2341 aaaacagtag ccacagcacc acacaccgca tgtcgtggaa cgatacgctg gatgaacgta
     2401 atagctgggg catgtctgcc ggactgcaat ccgaccgtcc ggacaatgga gcccaggtga
     2461 gcggtaacta tcagcatctg agttcagcgg gtgagtggga tatttctggt acctatgccg
     2521 ccaatgatta cagttccgtc agcagcagct ggagcggttc tttcaccgca acccaatatg
     2581 gtgcagcgtt tcatcgccgc agctccacca atgaaccacg cctgatggtc agcaccgatg
     2641 gcgtggcaga tattccggtt cagggcaatc tcgactacac caaccatttt ggcattgcgg
     2701 tggtgccgtt gatttccagt taccagcctt ccaccgtggc ggtgaacatg aatgacttac
     2761 ccgacggcgt aacagttgca gaaaacgtta tcaaagaaac gtggattgaa ggcgcgatag
     2821 gttacaaatc actggcttcc cgttccggta aagacgttaa cgtcatcatt cgcaacgcca
     2881 gcggtcagtt tcctcccctc ggagcggata tccgccagga tgacagcggc attagcgtgg
     2941 ggatggttgg cgaggaagga catgcctggt taagcggagt cgctgaaaat caaaagttta
     3001 ccgtggtctg gggtgatagc cagcattgct cgctccatct tcctgaacat atggaagaca
     3061 ccgcaaatcg cctgatttta ccttgtcatt aattaaggaa atgaaaatga catttatgaa
     3121 aggactaccc cttttgttgt tggttgccag tctgtgcagc cacgctgcac tacaacccga
     3181 tcgcactcgt attgtgttta acgctaatga taaagctacc agtctgcggg tggataatcg
     3241 tagcgataaa ctcccctacc tcgcttattc ctggctcgaa aatgaaaaag gggagaaaag
     3301 cgatgatctt ctggtcgccc tgccgccgat acaacgtctg gaaccgaaag caacaacgca
     3361 ggtacggata gtaaagcaag cctcgaccac aaaattaccg ggcgatcgcg aaacgctatt
     3421 tttctacaat atgcgtgaaa ttcctcccgc cccggaaaaa aacagcgacc atgccgttct
     3481 gcaagtcgcc attcagagtc gtatcaaggt gttctggcga ccggctgcat tacgcaagaa
     3541 agcaggagaa aaggtcgaac tgcagttaca ggtcagccag cagggcaacc aactgacact
     3601 gaaaaatcct accgcgtatt atctgaccat tgcctacctg ggacgaaatg aaaaaggcgt
     3661 tctccccggc ttcaaaaccg tgatggttgc gccttttagt acggtcaaca caaacatcgg
     3721 aaattatagc ggcagccagt tttatctcgg ttatatggat gattacggcg cgttacgcat
     3781 gacgacgctc aactacagcg gacaatgcca tttacaagcc gtggaggcga agaaatgagt
     3841 gctggcaagg gagtgttgct cgtcatctgt ctgttatttc tgccgttgaa gtctgcgctt
     3901 gccctgaact gctactttgg ttcatccggt ggctcggtag aaaaatcaga agcgattcaa
     3961 ccttttgctg taccaggcaa tgccaaactc ggcgataaga tttgggaatc tgacgatatt
     4021 aaaattcccg tctattgtga caacaatacc aacggcaatt ttgaaagtga gcatgtttat
     4081 gcctgggtaa acccctaccc gggggtgcag gatcgctatt atcaactcgg cgtaacttac
     4141 aatggcgttg attatgacgc caaccaggga aaaagccgca tcgacactaa ccagtgtatt
     4201 gacagtaaga acattgacat ttatacccct gagcagatta tcgcgatggg atggcagaac
     4261 aaaatttgct cgggtgatcc cgctaatatt cacatgtcac gtacctttct cgctcgcatg
     4321 cggttatacg tcaaaatacg agaaatgccg ccgcatgatt atcaaagtac gcttagcgac
     4381 tatatcgtcg tgcaatttga cggtgccggt agcgttaacg aagatcctac tgcccaaaac
     4441 ctgaaatatc atattaccgg tctggaaaac attcgcgtgc tggattgcag cgtcaatttt
     4501 tccatttcac cggaaacaca ggtgattgat tttggtaaat ttaatttgct ggatattcgt
     4561 cgccacacaa tgtcgaaaac attcagtatt aagacgacaa aatcacaaaa tgaccaatgc
     4621 accgacggat ttaaagtcag ttcctccttt tataccgaag aaacgttggt tgaagaagat
     4681 aaagcattgt tgattggtaa tggtctgaag ctacgtttgc tggatgaaaa cgcctcgcct
     4741 tataccttca ataaatatgc tgaatacgcc gatttcacca gcgacatgtt ggtctacgaa
     4801 aaaacctata cggctgaact ttcgtccatt gcaggtaccc ccatcgaggc tgggcccttc
     4861 gatacagtgg tgctttttaa aattaactat aactgatttg ccatgatgcc ctttgcagcc
     4921 ggaaagggca tctctttcag aaaattgctg attttcccct tttccttgat tgctgttatt
     4981 ttttcctggc ccggctttct ttatgctgta tcgatacacc aaagagaaaa taatgaggga
     5041 gcgaaggatg ccagttttgc agtggggaat gttatgtgtg ctatcacttc tcctttctat
     5101 tggtttcctc gcggtacatc tcccggcggc gctattactt gggccgatga tcgccgggat
     5161 catcttcagt atgcgcggaa ttactctgca acttccccgc tccgcttttc tcgccgcgca
     5221 ggccattctt ggctgcatga ttgcgcaaaa cctcactggt tcgattctca ccacgctggc
     5281 cgccaactgg ccgatcgtgc ttgctatttt actggtgacg ctgctttcca gcgccatcgt
     5341 gggttggtta ttggtgcgct atagctcact gcctggaaat accggtgcct ggggttcttc
     5401 tccaggcggc gcagcggcaa tggtcgctat ggcgcaagat tacggcgcag atattcgcct
     5461 ggtggcgttt atgcaatatc tgcgagtgct gttcgtcgcg ggggccgctg ttctggtcac
     5521 ccgcatgatg ctgggtgata acgctgaagc ggttaaccag caaatcgtct ggttcccgcc
     5581 agtgagcatt aatctcctgc ttacgatttt gctcgcagtc gttgccggta cggcgggatg
     5641 catgctgcgt cttccttccg gcacgatgct catcccgatg ctggcgggcg cagtgctcca
     5701 gtctggtcag ctcatcacca tcgaactccc ggaatggctg cttgcgatgg cgtatatggc
     5761 aattggctgg cggattggtc ttggtttcga taaacaaatc ttactgcggg cactacgccc
     5821 gctaccgcaa atcctgctgt cgatttttgc tctgttggct atttgtgcgg gtatggcgtg
     5881 ggggctgacc cggtttatgc atattgattt tatgactgcc tacctcgcca ccagccccgg
     5941 cgggcttgat accgtggcgg tcatcgccgc agggagcaat gccgatatgg cgcttattat
     6001 ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg ggacctgcca ttgcacggtt
     6061 tatttcaacc tatgcgccga agcggtcggc ttagtgctgg cagccagggc accagtaaaa
     6121 cgggcgagat gacagcgtgg ttttctcaat gatgctgcca caacgttcgc acggttcgcc
     6181 atctcgatga aaaaccttaa agcgaaacag cgccccatga tgcttattct catccacctg
     6241 cccccgcgta gcgtaagaaa atcgaggaat ctccagtaac gcgtgtgcga gtgcatccag
     6301 ttgcgccgca ttgagatctt tcgctttatg atttccagtc aacccaacct gccagaggat
     6361 ctccacccgc aaataattgc caagtcctgc cagaaacgcc tgctcgagca gtaatcctgc
     6421 aaactgacgg ttacgaaagc gcggcgacaa tacccgttct ttcaccacct ccggcgtcag
     6481 attcggatcc agcacatcgg gaccaacgcg ttgtaaaaac ggatgcgtgg tcagttgttc
     6541 cggggtcaac atctcaatat ccgaggcgct ataaagcaga atggttttgt cagccgtttg
     6601 cagttttacc cgcaatactc gcgtggtctg cggctcttcg ccggtatcaa ccacgcgcca
     6661 gacgccgtaa agctgattat ggctgtagag cgttaattcg ttggaaaaat gagttaacaa
     6721 cgccttacca cgcgtttcca catgggtaac gtgttgaccg ataagttgtg attgataagt
     6781 ttttaactgc gggaaggcaa accagacatc agttagtggt ttgcctttga tcgccgcctc
     6841 caggttatcc gctgcacggc ggatctccgg gccttcaggc atggtgatat ccttttttca
     6901 ttctttatta agcggggtta tgctgcgaca acaatcccct ttttggcaaa tgtagctcgt
     6961 aatcggcggg cgaacgccag agcgtgctcg ccgtcgccat gcaggcagac cgtttgcgcc
     7021 gcgaccgttg cccattcgcc ggtgatactt ttgactctgc cgtgttgcac catctccagc
     7081 gtttgcgcca gcgcctgttg ttcgttttca atcaacgcgc ctggctgact tcgcggcacc
     7141 agcgagccgt cagcctgata accgcgatcg gcaaacactt cctcgcgcgt tgtcaggcca
     7201 tattgcttgc ctgcacgaat cagctcgctt cctgccagcc caacgagaat caatgccgga
     7261 tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt gtgcttcttt cgccgcctgg
     7321 ttgtacaaca tgccgtgcgg tttgacatga cgcattacgc cgccttgcgc acgggcaatc
     7381 gttgccagcg cgccaatttg atacagcgtc tgggcgtaaa cggtttctgg cggcaactgc
     7441 atggcgctgc gaccaaaatt ttccctgtcg ggaaaactcg ggtgagcgcc aattgcaaca
     7501 ccatttttta ttgcttcacg cacgcaagcc tgcatggttt gcgcatcgcc tgcatgaaat
     7561 ccacaggcaa tattggcaga ggaaaccagc gttaatagct ctgcgtcgct ggcgcagcct
     7621 tcgcccagat cggcgttcag gtcaattttc attgtgcagc cgccacgcta attgttcaaa
     7681 ataacgttgc tgatcttgcc gcgctttcag tgcctcttcc agtgaacact ggacaaaatg
     7741 aatcggctga ccgagcggaa tttgcgcaag atggtacata tcagcctcaa tgatgcaggc
     7801 aatacgcggg taaccaccgg tggtctgtgc gtcgttcatc aacacaatcg gctgcccgtt
     7861 atgtggcacc tgcaccacgc ccggtaacaa accgtgagat aacagttcgc gatcggtggt
     7921 gcgttttaaa atttgcccct gtaagcgata gcccatgcgg ttactttgcg agctaagctg
     7981 ccagggcgaa cgccagaatg catcctgcga ggcgcgatcg aactcatgat attccggccc
     8041 cggcaaggcg cgaatgcggt tgccccacag cagctgttta acgccctgcg cttccataaa
     8101 atcacgcttc gctttgccaa tcgggagtcg gtcaccatcc ttcagtaaac ggccttccag
     8161 cccgccactc cccactttga gatcggtgct gcatgacccc attaccggcg gaacatcaat
     8221 accacccgcg accgccagat aactgcgcat cccgtgctgc gggcgtttaa gcgttaaacg
     8281 ctggcctgct ttcatcggca atcgccagcc ggtccagacg gcattatcat ccagccgcgc
     8341 ttcgcaaccg gcacccgtca gagcaaacca cccatcagtt tcgaactcaa cagttaactg
     8401 accgagcgtg atctccagcg cgggggcatt ggcgtcatta cccaccagta ggttagcaat
     8461 gcgtaacgcg ggcatatcca gtgcgccgca gtggctgata cccgactggc gaaaaccgtg
     8521 acgaccgcca tcctgcacag tggtatacat gcccgcacga ataatcttca gcatactccc
     8581 tccttctgcg gtacaaagcg cacgctgtct cccggacgta ataagatggg ttcgtcacgc
     8641 gccggatcaa acaggctgag tgaggtatga ccaatcaact gccagccacc cggcgttgcc
     8701 agcggataaa caccagtctg cggcccgccg atcccgacag aacctgccgg aacgagtaag
     8761 cgcggttcag cgcgccgtgg cgtgtgtagt tgttccggca aactcccgag atacgggaag
     8821 cccggttgaa aacctaaaaa ccagaccacg tattccacgg aggagtgcaa ttcaacaacc
     8881 tgtttttcgc tcaacccgca atgcgccgcg accaccgcca aatccggtcc gcctgcacca
     8941 ccgtaaacca ccggaatttc aataaagcga gactcaggtt ccagggcctc gctctcctcc
     9001 caccagcgtt gcaaacgctc tatggcatcc agcgccagcg actcaggatt acgcaaaatc
     9061 accgtaatat tgttcatgcc gggaatggct tcaaccacat tcggcatatc caccagacgc
     9121 tgcgccagtc gccagatccg tttctggcta gccagcgtca ccggcggttc cagttccagc
     9181 actaccgccg tttcacctat cagataacaa cgcgctcgtt gcactttcga tccctctgat
     9241 tattcgttat gcaggattag gaatatcaat aaaggtcaca tcaagatcgg tattttcatt
     9301 cagccactcg ctcaatgcgc gaataccacc acgttcagtg gcatggtgac ctgcagcata
     9361 aaaatgcaat ccctgctcgc gggctgaatg aatggtctgt tcagaaactt cgccagtaat
     9421 aaaagcatcc acgccaaaac gcgcggcgct atcgataaaa ctttgcccgc cgcccgtgca
     9481 ccaggcgacg cgctggacca cctcaggtcc ggtatcgcca caccataacg gcttacgtcc
     9541 cagacgcgct tcaatccagg aggctagttc cagtcccggc acaggcatgg tcagctctcc
     9601 ccacggcacc aacggctcaa tttcgcccat gacagtgatc cccagtaacg ccgccagctg
     9661 tgcgttattg cccagctcag gatgtgcgtc aagcggcaaa tgccagccat acaggttgat
     9721 atcattcgcc agcaacgttt ttaaacggtt acgcttcatt ccgcgaatga ccggagactc
     9781 tcctttccag aagtagccat gatgcacaat gaccgcatcc gcgcccagac gcactgcctc
     9841 atcgagcaaa gcctggctgg cggtgacacc ggtaacaatt ttttgcaccg tctctttgcc
     9901 ttccacctgc aaaccattcg gcgcgtaatc actaatcgcc gcgctgttca gtttttcgtt
     9961 aatcagttgt tccagttcgg tgtttttcat cttcgcgcct tccgtattct gatagctaca
    10021 ccgttaacat agcgttacct cgcctgttcg tcgatagcca atcttttgac atattaagaa
    10081 acaaacccga cgccaaaaga cgcaaattgt ttatatcatc acgcttcagc taatgtatat
    10141 ttatgcaatc agacagcata ttgcataaat aatcagatct gtatcaaagt tttaacttta
    10201 aggaaacaca agcacgccgc gcaactatta cgtgacgttg ccgtcaattg tggaattatt
    10261 atgaataaac acgcatcaca accgcgcgct atttactatg tcgttgcgct gcaaatctgg
    10321 gaatatttta gcttttacgg catgcgtgcc ctgctgattc tctatctcac caatcaacta
    10381 aaatacaacg atactcacgc ctacgagtta tttagcgcct actgttcgct ggtgtatgtc
    10441 acgccaatcc tcggtggctt tttggcggat aaagttctcg gcaatcgcat ggcggtgatg
    10501 ctgggggcgt tgttgatggc gatcggtcat gtggtgctgg gtgccagtga gatccatccg
    10561 tcattcctct atctgtccct ggcgattatc gtttgtggct atggtctgtt taaatccaat
    10621 gtcagttgcc tgctcggcga gctgtatgag ccaaccgatc cgcgtcgtga tggcggtttc
    10681 tcgctgatgt atgcagcggg taacgtgggg tctattatcg cacctatcgc ctgtggttac
    10741 gcccaggaag agtacagctg ggcgatgggc tttggcctgg cggcggtggg tatgatcgcg
    10801 ggtctggtca ttttcttatg tggcaatcgt catttcacac atacccgcgg cgttaataaa
    10861 aaagtactgc gtgcaacaaa ctttctcctg cctaactggg gatggctgct ggttctgctg
    10921 gtggcaacgc ctgcgctgat taccgtactg ttctggaaag agtggtcggt atacgcctta
    10981 attgtcgcga ctattattgg cctgggtgta ctggcaaaaa tttatcgcaa agcagaaaac
    11041 cagaaacagc gtaaggagct ggggctgatt gtgacgctca ccttcttcag tatgttgttc
    11101 tgggccttcg cacaacaggg cggcagctcg attagccttt atatcgaccg cttcgttaac
    11161 cgcgatatgt ttggttatac cgttccgact gctatgttcc agtcgattaa tgccttcgca
    11221 gttatgctgt gcggtgtgtt cctggcgtgg gtggttaaag aaagcgtcgc aggtaatcgt
    11281 accgtgcgca tctggggaaa atttgctctt ggccttggcc tgatgagcgc cggattctgc
    11341 attctgacct taagcgcccg ctggtccgca atgtatggtc actcttctct gccactgatg
    11401 gtattaggcc tggcggtgat gggctttgcg gaactgttta tcgacccggt tgccatgtcg
    11461 caaattacgc gcattgaaat tccgggcgtg accggcgtat taaccggcat ctacatgctg
    11521 ctttctggcg cgatcgcgaa ctatctggcg ggcgtgattg ccgatcagac atcgcaggct
    11581 tcgtttgatg cttccggggc gatcaactac tccatcaatg catatattga agtatttgat
    11641 caaattacct ggggcgcact ggcgtgtgta ggactggtac tgatgatttg gctgtatcag
    11701 gcgctgaaat tcagaaaccg cgcgctggcg ctggagtcat aataggccag caccatacct
    11761 gataaaacgc tcgctgcgtt ttatcaggct cctttcctcg cttcttcgta cgctgccagc
    11821 gttcgcagcc ttgcctcttt gtgatcaact atcggttgcg gataatccag catcacacct
    11881 gctttctgcg cccacttcca cggctcatgc accgctttcc ctggtacatc gcgcagctcc
    11941 ggtaaccatc ggcggataaa ctcaccttcg cgatcaaatt tctcgccctg ggttgtcggg
    12001 ttgaaaatac gaaaatacgg cgctgcatca gtacccgtag acgccgccca ctgccagcca
    12061 ccgttattgg ctgccaaatc accatcaatc agctgcgaca tgaaatatcg ctcgccttcg
    12121 cgccagtcga tcaacaaatc tttcaccaga aaactggctg aaatcatccg tagccggtta
    12181 tgcatccagc cagtgctgtt aagctgacgc atggcggcat cgacaatcgg gtatcccgtt
    12241 ttgccttcct gccaggcctg taaatgtgcg ggattgctct gccactgcac acgatccgtc
    12301 caggcaataa acggacaatg tttacacaac gaggggtaat acgtcatcag atgacggtag
    12361 aactcgcgcc agatcagctc actaagccag acactaccgg ccccaccgtc cagcgcctgc
    12421 ggctgttccg ccaacaagcg atgcaaacac tggcgaggcg ataacccgcc cgttgccagg
    12481 ctggcggaca aacgactggt gccttccact gccggaaaat ctcgttgttg ctcatattct
    12541 ccggcaccgt tctggcaaaa ctggcgtaat tgcgcaatcg ccgctttttc ttccaccggg
    12601 aaatgcgcag tatcgaaaga ctgacgagga taattcagcg taatggatgg cgagggctct
    12661 atcgatccgc tactacgaac ttttggcgca gcgacgcact ccggcatccc ttcccgcagc
    12721 cgtttcagcc aggcattctt aaaaggcgta aagactttgt acatctcgtg attaccggtc
    12781 ttcaccgcgc caggcggcag gatcacgctg tcatcaaatc cttcacacac cacgttacgc
    12841 agcgctcttt caacttccac atcccgcgcc cgctcattca cttcatactg atagttataa
    12901 aacaggtggg taacgctgtt ttccgcgcac acctgtttaa ctatttcgac actggcgaca
    12961 aagtcatcca cttcacggaa caataaagga ataccttttt ccgcaagcgc tatttgtagc
    13021 ccattcagtt gagcattgat aagttcagcc tgacgcggcg acatgttatg cgtcgcccac
    13081 tggcgtggtg tagcgatata caacgccagc acgcgtgcag acgaattgcg gcaggcggca
    13141 gccagtgcga gattatcgtg cagacgtaaa tcctggcgaa accagaccag atgggtagtc
    13201 ataaaactcc tgaatggcgc aagttaatca cacgcggcca aagttcgaaa taacgctgcc
    13261 ctgaaagcca ggcgtcagga taaagcgcca tatagtgttt gatacgcatc agcggcgcaa
    13321 gaagtggttg ctcgccacgg cgataactgt cgataagcgc cgccaactgc tggcgttctg
    13381 tggaatcaat atgcgggcga aaataaccct gaacgtgcat caaaacgttg gtgtgatcgc
    13441 gcgggttggc gggatgagaa agcaaaacaa ttacgcgttg gtagtactgg ttgtaaaaat
    13501 catccagatt ttgccactgg tgaatatcag ccacgaacgg gccaagtttg cggtactccg
    13561 gctgagaatg cgccagaaag acgagtttat agcggctgtg ataatcgaga agcgccccgc
    13621 gcgttaagcc atgttctttc agtcgatgta actcatgaag ggcgaagata cggattaggg
    13681 tgctgtcatc aaatcgttgc tgattcatta ctgctcctga acggtttaca ggagtaagtg
    13741 tagacgttga gaaatgatat ttgtgaagtt aatcctgttg tagcaggaca aatttacgaa
    13801 agcctgctcc ccgcaaggac tgacgtcagg tagtttctaa ccatagctgc cctttctatt
    13861 caaacgtgaa atcaataagc tcatcaggga catcgcaaac aatatgaaga cgggtttctt
    13921 ctctgccgtg actcttttca ctaattgcgt aactgtcatg cttgggatta tttaattctt
    13981 tcagcggaaa tttttcctcg aaggctttat ttagccgccc ctggtttcct tttacagcga
    14041 ataaataatc acctccctgt ttttgtatct tctctgcaat atctttctgg caacccatcg
    14101 catcggttgt gatgattttt cctttaatat ccagcatgtt aagaagttct gggatagctg
    14161 tgatctcatt ggatttctta tccgtcttga tctgcccgat gaccagactg tgcattgttg
    14221 agaa
//
LOCUS       DAZGLJ010000119        14188 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000119 DAZGLJ010000000
VERSION     DAZGLJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.119"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(35..115)
                     /gene="shoB"
                     /locus_tag="ACHK49_003057"
     CDS             complement(35..115)
                     /gene="shoB"
                     /locus_tag="ACHK49_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO9263670.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(535..915)
                     /gene="acpS"
                     /locus_tag="ACHK49_003058"
     CDS             complement(535..915)
                     /gene="acpS"
                     /locus_tag="ACHK49_003058"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO9263671.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(915..1646)
                     /gene="pdxJ"
                     /locus_tag="ACHK49_003059"
     CDS             complement(915..1646)
                     /gene="pdxJ"
                     /locus_tag="ACHK49_003059"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO9263672.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1658..2386)
                     /gene="recO"
                     /locus_tag="ACHK49_003060"
     CDS             complement(1658..2386)
                     /gene="recO"
                     /locus_tag="ACHK49_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO9263673.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2398..3303)
                     /gene="era"
                     /locus_tag="ACHK49_003061"
     CDS             complement(2398..3303)
                     /gene="era"
                     /locus_tag="ACHK49_003061"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO9263674.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3300..3980)
                     /gene="rnc"
                     /locus_tag="ACHK49_003062"
     CDS             complement(3300..3980)
                     /gene="rnc"
                     /locus_tag="ACHK49_003062"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO9263675.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4253..5227)
                     /gene="lepB"
                     /locus_tag="ACHK49_003063"
     CDS             complement(4253..5227)
                     /gene="lepB"
                     /locus_tag="ACHK49_003063"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO9263676.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5243..7042)
                     /gene="lepA"
                     /locus_tag="ACHK49_003064"
     CDS             complement(5243..7042)
                     /gene="lepA"
                     /locus_tag="ACHK49_003064"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO9263677.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7240..7719)
                     /gene="rseC"
                     /locus_tag="ACHK49_003065"
     CDS             complement(7240..7719)
                     /gene="rseC"
                     /locus_tag="ACHK49_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO9263678.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKVSCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7716..8672)
                     /gene="rseB"
                     /locus_tag="ACHK49_003066"
     CDS             complement(7716..8672)
                     /gene="rseB"
                     /locus_tag="ACHK49_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO9263679.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8672..9322)
                     /gene="rseA"
                     /locus_tag="ACHK49_003067"
     CDS             complement(8672..9322)
                     /gene="rseA"
                     /locus_tag="ACHK49_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO9263680.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9355..9930)
                     /gene="rpoE"
                     /locus_tag="ACHK49_003068"
     CDS             complement(9355..9930)
                     /gene="rpoE"
                     /locus_tag="ACHK49_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO9263681.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9927..10082)
                     /gene="rseD"
                     /locus_tag="ACHK49_003069"
     CDS             complement(9927..10082)
                     /gene="rseD"
                     /locus_tag="ACHK49_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO9263682.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10338..11960
                     /gene="nadB"
                     /locus_tag="ACHK49_003070"
     CDS             10338..11960
                     /gene="nadB"
                     /locus_tag="ACHK49_003070"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO9263683.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(11945..12682)
                     /gene="trmN"
                     /locus_tag="ACHK49_003071"
     CDS             complement(11945..12682)
                     /gene="trmN"
                     /locus_tag="ACHK49_003071"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO9263684.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12814..14148
                     /gene="srmB"
                     /locus_tag="ACHK49_003072"
     CDS             12814..14148
                     /gene="srmB"
                     /locus_tag="ACHK49_003072"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO9263685.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
BASE COUNT         3404 a         3805 c         3543 g         3436 t
ORIGIN      
        1 ttcaccggac gcaccttaag tacgtttcct tgtgttataa gaacagaagg atcagctgta
       61 aaacagcaat gatgattttg atgacccgtt taatcaggta tcggcaatca gtcattcgtt
      121 tttccttaaa caaggaatgc agccgttcag attagccctt acatctcccc aaaactgaac
      181 gtgcgagtta ttgagggtgc atgctgcact ccacaccaga gctttgacga caccactcgt
      241 ttcaatgggg gaattctgtg gcatggtgta aagcacagca aaatcttcaa taacgaagcc
      301 aattttaatg tacttacgaa ttggcggtca cgttaatctt tccatcaata ttgctttctt
      361 cgtaaaggct cgagttttta tgctaaagat tgcaagttgc ttgtaaaaga taagtacact
      421 gatccataat cgctgttgtt gagggtgcat gctgcacaaa attaaagtta aaaagtaaaa
      481 cccccgttcc ttaccagttc gggggtttta ctttttaaag agaacggtat tattttaact
      541 ttcaataatt accgtggcac aagcatagtg gcgttcatct gccagcgtta catgcatatt
      601 tacaacgccg agcttttccg ccagttttaa tgcctcaccc catagccgta gccgtggttt
      661 gccgagctca tcattgaata cttcaaattg attaaacgcc agaccattgc ggatcccggt
      721 gccaaacgct tttgctgcgg cttctttcac agcaaaacgc ttcgccagaa aacgcaccgg
      781 ctggtggtgc gttttccaga ttgcccattc gttatcgctt aatacgcggc gtgccaggcg
      841 ctcaccggat cgggcgatca ctgcttctat gcgagcgatc tctacgatat ccgtgcctaa
      901 acctaatatt gccattagcc acgcgcttcc agcatcagac gcttcatttc tgccaccgca
      961 tctttcagtc cggtcatcac tgcacgacca ataatggcat gaccgatatt cagttcatgc
     1021 atctcaggga tggcggcaat agctttcacg ttgtgatagg tcagaccgtg accagcgtta
     1081 actttcagac cgagactagc ggcaaaggtc gcggctttgg cgatacgcgc cagctcttgc
     1141 gcctgttcgg cgtcagtttt ggcatcagca tagcaaccgg tgtggatctc gataaacggt
     1201 gcgccaacct ctgccgcagc tttgatctgc tcttcatcgg cgtcgataaa gagagaaacc
     1261 tggatcccgg catctgccag acgtttgcag gcatcgcgca ttttgtcacg ctgccctgcg
     1321 acgtccaggc cgccttcggt tgttacttcc tgacgctttt ccggtaccag acagcaaaaa
     1381 tgtggcttcg tctcaacggc gatcgccagc atctcttcgg tcaccgccat ctccagattc
     1441 atgcgggtat ccagcgtctg acgcaggatg cgcacatcac ggtcagtaat gtggcggcga
     1501 tcttcacgta aatgcacggt aatgccgtcc gctcccgcct gctcagcaat aaacgccgcc
     1561 tgcaccggat ccgggtaagc agtaccgcgt gcgttacgca gcgttgcaat atggtcaatg
     1621 ttgacgccta acagtaattc agccatgaca atcctcatca ttcataatgt gttttcaccg
     1681 ttcgcttagg cataaactgc cggaacagtt ccctgctctt taatggttta ccgccaagat
     1741 acggcttaag cgccatgcgg gtaaagcgtt tcgcggcgcg tagtgtgtct gcgtcaggaa
     1801 attcccgcgc gtttaacgct tttaactgcc ttccggtgaa cgttttattg tcgataacga
     1861 cgcttgcgat aaaccctttt tcttcgcgat aacgatacgt catggtgtca tctaccggct
     1921 cgccgctacc cgcacaatgg gtaaaattga cgccatagcc cagatgcccg agcagtgcca
     1981 gttcaaagcg gcgcagcgcg ggttctggcg taccagtggc acctgcaaga gactgaatgc
     2041 agtgcaagta atcgaaaaag agttcagaga agcgcgtctc gtattccagt acgcgggaga
     2101 gaagttcgtt gatgtacaga ccgctgtaaa gcgtgatacc gcttaatggc agcgccagcg
     2161 agacagcttc agcactgcgc agcgttttga cttcgccacg cccgccgaaa cgtagcaaga
     2221 gaggggtgaa aggctgtaat gcacctttca gagtagagcg tttagagcgt gcgcctttgg
     2281 caaccagacg cacgcgaccc gattcctccg tgaagacgtc cagcatcagg ctggtttcgc
     2341 tccacgggcg actatgcagg acaaatgcgc gctgccagcc ttccatcgga gtgactctta
     2401 aagatcgtca acgtaaccga gactgcgcag tgcgcgttcg tcgtcggccc aaccggattt
     2461 cacttttacc cacagttcaa ggtggacagg cgcttcgaac atttcctgca tgtctttacg
     2521 cgcttcaatc ccgatggttt tgatcttggc ccctttgttg ccaataacca tcttcttctg
     2581 cccttcacgc tcaacgagaa tcaaaccatt gatgtcataa ccaccgcgtt cgttagagac
     2641 gaaacgttcg atctccacgg tcacggagta cggcagttca gcgccgagga aacgcatcag
     2701 tttttcgcgg atgatttcag acgccataaa acgctgtgag cgatcggtga tgtaatcttc
     2761 cgggaagtga tgggtcgctt cagggagatg cttacgcacg attgctgcga tagtgtcgac
     2821 attcagcccg gtttcagcag agatcggcac gatatcgagg aagttcatct gacttgccag
     2881 gaactgtaag tgcggcagca gatcggcttt ttcctgcacg ttatccactt tgttcaccgc
     2941 gaggatcacc ggcgctttgc catcgcgcag tttgttgagc accatttcgt cgtccggcgt
     3001 ccagcgggtg ccttcaacga caaaaatcac cagctcaacg tcgccaatag agctgctcgc
     3061 cgctttgttc atcaggcggt taatagcgcg tttttcttcc atatgcaggc caggggtatc
     3121 aacgtagatc gcctgatacg cgccttcagt atggatcccc acaatgcggt gacgagttgt
     3181 ttgcgctttg cgggaggtga tggagatctt ctgccccagc agtttgttca acaatgtgga
     3241 tttgccaacg ttcggacgtc cgacgatggc aataaatccg cagtaacttt tatcgatgct
     3301 cattccagct ccagtttttt caacgcctgt tcggcggcag cctgctcagc cttacgacgg
     3361 cttgaacctg tgccaaccac cggttcactc aggccgctga cctggcagtg gatagtaaat
     3421 tcctgatcgt gcgcttcgcc acgtacctgg actaccagat aagtcggcag cggcagatgg
     3481 cgaccctgca aatattcttg caagcgcgtt ttcggatctt tttgtttatc gcctgggcta
     3541 atttcgtcca aacgagtttg ataccagttg aggattaatt tctcgacggt ttgaatatca
     3601 ctgtcgagga atacaccacc aattaatgct tcgacggtgt cggcgagaat tgactcacga
     3661 cgaaatccac cgcttttaag ttcacctggc cctaaacgta agcactcgcc taactcaaat
     3721 tcgcgcgcca gttccgccag cgtattgcca cggaccagcg tggcgcgcat ccggctcata
     3781 tcgccttcat ccacacgagg gaaacggtga taaagcgcat tggcgataac gtagctcaga
     3841 atagagtcgc ctaaaaattc taaacgctcg ttatgtttgc tgctggcact acgatgagtt
     3901 aatgcctgct gcaacagttc ctgatgatta aaagtgtagc ccagcttccg ttgaagccga
     3961 ttaattacga tggggttcat gcgataccaa taaataaatg cgtcaacaat tcagcacacg
     4021 aaacagacct gatatacatg gctctgctaa ctgcttcgct gcagtttcag tttaccggta
     4081 tatggggacc aacgctgttt cgtgtgccgt ggcaacctgg aggtgccaac cttaaacttc
     4141 gggggaatat tctatacaca acgacggggg atgtcgttag ccacgggaga tttatctcat
     4201 aaataattca cgttgtcgcc ataacggcga caacgtgaac gaagatgggc tattaatgga
     4261 tgccgccaat gcgacttaag cgcacacctg tcggccattc gccttcttgc ttatcgaaac
     4321 tcatccagat agccgttgcc cgaccgacca gattcgcttc aggcacaaaa ccccagtaac
     4381 ggctgtccgc gctgttgtcg cggttgtcgc ccatcatgaa gtattgtccc ggcggaacaa
     4441 tccaggttgc cagttgttgc cctggctgct ggtaatacat ccccacctgg tcctgcgcaa
     4501 tcggcactgt cagaattcgg tgcgtcacat cacccagtgt ctctttacgc tcggaaagac
     4561 gaattccatt ttctttggtt tcgtttttcg gcacttcaaa gaatccgctg gtcgcttccc
     4621 caccattacg gcgtgagaag gtctgaacga aatcgctcgg ttccacgttg gagtaggtga
     4681 ccggtagcgc gttttcacac gcctggccgg aactgcatcc cggttgaatc gtcagctctt
     4741 ttgagaccgg atcgtaagtg actttatcgc ccggtaaacc caccgcgcgc ttgatgtaat
     4801 caagctttgg atcttccgga tatttaaaga ccacgatatc gccgcgtttc ggatgaccgg
     4861 tttcgatcag cgttttctgg tagataggat ctttaatgcc ataagcaaac ttctctacca
     4921 gaataaaatc accgattaac agagtcggca tcatcgaacc tgacgggatc tggaacggtt
     4981 cataaataaa cgaacgtaca atcaatacga tagccagcac cggaaaaaca gaagcaccgg
     5041 tttccagcca gccaggcttc ggcgcaacct ttttcagggt tgctttatcc agtgagtcac
     5101 cggcagccgc ctgcgccgct gcctgacgtt cccgccgttt aggtgcgaaa aagaatttat
     5161 ccacgcacca taaaatgccc gtcaccagtg tggcaatcac cagaatcagg gcaaacatat
     5221 tcgccatgcc aactcctaag ggttatttgt tgtctttgcc aacgtgcaga atggcgagga
     5281 acgcttcctg cggcagctcg acgttaccga tctgcttcat gcgtttctta ccttctttct
     5341 gcttctgcag cagctttttc ttacggctga tatcgccgcc ataacattta gccagtacgt
     5401 ttttacgtag ctgtttcacg gtggatcgcg caataatgtg cgtaccaatc gctgcctgaa
     5461 tggcgatatc aaactgctgg cgtgggatca gatctttcat cttctccacc aactcgcgac
     5521 cgcggttttg cgaattatca cggtgggtga tcagcgccaa cgcatcaaca cgttcaccgt
     5581 tgattaatac gtctacacgt accatgtcgg acgcctggaa gcgtttgaag ttgtaatcca
     5641 gagacgcata accacgcgag gtagatttca ggcgatcgaa gaagtcgagc accacttccg
     5701 ccatcgggat ctcgtacgtc agcgccacct gattaccgtg gtaaaccata ttggtctgca
     5761 cgccgcgttt ttctacgcac agcgtaataa cgttgccgag atatgcctgc ggcagcagca
     5821 tgtgacattc ggcaatcggc tcgcgcagtt cgtagatgtt atttaccgct ggcagcttgg
     5881 atggactgtc gacgtaaata acttctcttg acgtggtttc aacttcatac actacggtcg
     5941 gcgcagtggt gatcagatcc agatcgtatt cacgttccag acgttcctgg atgatctcca
     6001 tgtgcagcag gccgaggaag ccgcagcgga aaccaaagcc cagcgcgctg gagctttccg
     6061 gctcatagaa cagtgaagca tcgttcaggc tgagtttacc cagcgcgtca cggaaggctt
     6121 catagtcgtc ggaacttacc gggaacagac cggcgtatac ctgcggtttg actttcttaa
     6181 agccaggcag cgccttttct gccggattac gcgccagcgt taaggtatcg ccgactggag
     6241 cgccgtggat atctttaatc gcacatacca gccagcctac ttcgccacat ttcagttcag
     6301 tgcggtcaac ctgtttcggc gtgaagatgc ccagacggtc ggcgttatag gtctgcccgg
     6361 tactcatgac tttcactttg tcgcccttac gcagggtgcc gtttttaata cggataagtg
     6421 aaacaacgcc caggtagttg tcgaaccatg agtcgataat tagtgcctgc aacgggcctt
     6481 ccggatcgcc ttccggcggc ggaatgtcgc gcaccagacg ttcgagaacg tcctgcacgc
     6541 caacgccggt tttcgctgaa cagcgcaccg cgtcggtggc gtcgatgccg acgatatctt
     6601 caatttcttc cgccacgcgt tcaggatcgg ctgccggcag gtcaatcttg ttcagtaccg
     6661 gcaccacttc gagatccatt tccatggcgg tgtagcagtt tgccagggtt tgcgcttcta
     6721 cgccctgccc ggcgtcgacc accagcaatg caccttcaca ggcagccagc gaacgggaaa
     6781 cttcatagga gaagtcaacg tggcccgggg tgtcgataaa gttcagctga taggtttcgc
     6841 cgtcagacgc tttgtagtcc agcgtcacgc tttgcgcttt gatggttatg ccacgctcac
     6901 gctcaagatc catggaatcg agaacctgcg cctccatttc acggtcagac aggccaccgc
     6961 agatctggat aatacggtca gacagcgtcg atttaccgtg gtcaatgtga gctatgatcg
     7021 aaaagttacg tatattcttc ataaagtatg attattgtgc cttaatgccc ggttaaccag
     7081 gcttttagaa gtcgctgttc tgagcttaac gtctgtatta atagaaacgc cgcattctac
     7141 actacaacat tgaggcgagg aaatgttcat accgtatgga ttgtggtatc tggaaacgtc
     7201 ctcgcatttg ttatgcaaaa tgcaacaaag ccagtgaaat cactggctcg cgtcttccga
     7261 agatgtttca aatcgcacaa ggccaggcgg caatgccacg cttaagatga tcggttgcca
     7321 ttctgcccgg gcagcaaact tacgcgagta gccgcgcgca atcaggaacc cgccaatacc
     7381 gccgaggacc gcaccacata atgctgcgac gtcagaagca aagagtagct gaaatagcga
     7441 agcgatgagg aataatccca ccagcggcga catataaacc agtaatgcgg agctaagcag
     7501 gctgccttcg gcgatcccca attccacttt ttgccccggc actaacggct catcacaggg
     7561 tacgacaatg gtatgcgtgg tttgcgggcc aagtttattt aacacgcggc taccgcaacc
     7621 ggcgcgtgaa gcgcagctgc tgcatgaaac tttaacatca caactgacca gcgcctgccc
     7681 gttttgccag gagacgacgg tagcccactc tttgatcatt gcgctgcccc gaacttaata
     7741 ttctcggcaa tgcgtttcgc cgtttgcggc ggcagttcac cgacaatggt gatttcggcg
     7801 ttatcacgta cgcttgtact gacggttctg cgtccggtgc gcaacatctg atcggtgctc
     7861 gatggcgtag cgcggttaac gtttaccgag aagctgaata atccgtcgga atagagacgt
     7921 gattcgatag gcatgttgtc catcgtcggt agcggacgtc gactactgga aacttcgcta
     7981 aaaccctgtg gcaaccaggt tggcgtccag ctgaatttag ctttttcacc tacaggaaca
     8041 gaaagcaacg gcggcaaatt tgccttcgcc agcgtctgca tactgctact gatatcctga
     8101 ttgacgttaa aagcaatcac gcgaaattgt tccagcgttt caccatcgcg atcaaggaga
     8161 tcaaccctca tcggtaattt cgattcgatg tccatccaca cgatgtagct gtagcgtgta
     8221 ccatctcggg caaccacgcg aatgacttcg caaagacgat cagcaatacg cgtgcgcccg
     8281 acggagataa agtcgtagta aggagaaagg cgtttgaaat cggtatagat gagcgatggc
     8341 agagaatcaa caatgtaatc gccattaagc gtgaacggtt caagtcccgg ttcaaaatag
     8401 ctgatttcat tgccacgctg taccacttcc cggcgcgggc catccatttg caacaattgt
     8461 gcaagaggac ggttatcgag gcgtgcatgt cgataacgca gagactcaac accctgttta
     8521 ttgatgctga tgaatgacag ctcgtaattc agtgactgac tggccaggtt catctgctgt
     8581 aataacgccc cggacgcggg agtggccgag gcgttagcag agaataacag gctacctgtc
     8641 actaatgaca tggcaaacca aagttgcttc attactgcga ttgcgttcct aaagtttgaa
     8701 ttcctggcac ctgtacagcg gcttgctggg tttgcgcctg ctcaaactga agctgttcag
     8761 agtggagtcg gcgttgcagt tcgtaatcct gcaacattgc attaatgcga cgacgctgct
     8821 cctgtacctg ctgctgttga ccattgtttg cggtcgcttc agaaggtact cccaggctta
     8881 ccgggctggc tttacccatc atcggcagtg tattaaatac cggcgtttcg ggctgctggg
     8941 acgtttcaga ttgtccatta tagtgctgga cgccaacgat aactgcaagc gatacgcatg
     9001 ctgctacgcc catttgggta agctgtgccg cccacggacg tactttctgc cagaatggca
     9061 ttttctgcca ttgatgcggc gcaggctggg cttccgggat caatgtcgcc ggttgacgta
     9121 ctggctcttc ttcaatggcg gccatcacgc gtgaagagat atcgaaatgg agcacctcgg
     9181 gagtatcacc ccgcattgag tcacggatta agtgatagct ttcccaggtt ttctgcattt
     9241 ctgggttatg agccagttcg ttaagcagct cactatccag cgtttcgcca tccattaaag
     9301 cggaaagttg ttctttctgc atgcctaata cccttatcca gtatcccgct atcgtcaacg
     9361 cctgataagc ggttgaactt tgttatcaat agcttccctc gctcggaaga tacgtgaacg
     9421 caccgtacct accggacaat ccatgatagc ggctatctct tcatagctca ggccatccag
     9481 ctcccgcaag gttattgcca tgcgcaaatc ttccgggagg gactcaatag ttcggaaaac
     9541 tatctgtctc agttcttctg acaacattaa gttctcaggg ttcgaaattt ctttcaacgc
     9601 gccgccactt tcgaagtttt cagcttcaat ggcatccaca tcactggaag gtggacgacg
     9661 cccctgagca accaggtaat ttttcgctgt atttacagca atccgataca gccatgtata
     9721 aaaagcgcta tctccccgga acgaatccag cgcacgatag gctttaataa aagcttcttg
     9781 taccacatcg ggaacatcac ccgacggcac atagcgggaa accagactcg ccactttatg
     9841 ctgatagcgc actaccagta ggttaaaggc tttctgatct cccttctgga cccgttcaac
     9901 caggacctgg tccgttaact gctcgctcat ccgaggtaaa gtctccccaa accaaatttc
     9961 cacgcgctat cgaaacgcca ctccattagc tgcaatttga gcaagcaaag ggttagagtg
    10021 tctcgttttt gtaaagttcc gtaacgcatc tgtttttgtt tgtcatgctg tagacggatc
    10081 attatctatc attataagtc tacagaatct gaacatcgca ttatctgtgt agaaatgccc
    10141 atttaactgc ctgaagagta acccaacggc ctttttattt caccacctaa tcctccacca
    10201 gccagtaact tctctttttc tcgccgccct gcgtcagcgt gtttagccac tgtaacaaat
    10261 attaaaatag caggtgttta ttcgcacaac atgatgctat gctgaccaaa cggtgtttag
    10321 taaattaaac aaagaaaatg aatactctcc cagaacattc atgtgacgtg ttgattatcg
    10381 gtagcggcgc agccggactt tcactggcgc tacgcctggc tgaccagcat caggtcatcg
    10441 ttctaagtaa aggcccggta acggaaggtt caacatttta tgcccagggc ggtattgccg
    10501 ccgtgtttga tgaaactgac agcattgact cgcatgtgga agacacattg attgccgggg
    10561 ctggtatttg cgatcgccat gcagttgaat ttgtcgccag caatgcacga tcctgtgtgc
    10621 aatggttaat tgaccaaggg gttttgtttg atacccacgt tcaaccgaat ggcgaagaaa
    10681 gctaccatct gacccgtgaa ggtggacata gtcaccgtcg tattcttcat gccgccgacg
    10741 ccaccggtag agaagtacaa tccacgctgg tgagcaaggc gcagaaccat ccgaatattc
    10801 gtgtgctgga gcgcagcaac gcagttgatc tgattgtttc tgacaaaatt ggcctcccgg
    10861 gcacgcgacg ggttgttggc gcgtgggtat ggaaccgaaa taaagaaacg gtggaaacct
    10921 gccacgcaaa agcagtggta ctggcaaccg gcggtgcgtc gaaggtttat cagtacacca
    10981 ccaatccgga tatttcttct ggcgatggca ttgctatggc gtggcgcgca ggctgtcggg
    11041 ttgccaatct cgaatttaat cagttccacc ctaccgcact gtatcaccca caggcacgca
    11101 atttcctgtt aacggaagca ctgcgcggcg aaggcgctta tctcaagcgc ccggatggca
    11161 cgcgttttat gcccgatttt gatgagcgcg gcgaactggc cccgcgcgat attgtcgccc
    11221 gcgccattga ccatgaaatg aaacgcctcg gcgcagattg tatgttcctc gacatcagcc
    11281 ataagcccgc cgattttatt cgccagcatt tcccgatgat ttatgaaaaa ttgctcgggc
    11341 tggggattga tctcacacaa gaaccggtac cgattgtgcc tgctgcacat tatacctgcg
    11401 gtggtgtaat ggttgatgat catgggcgta cggacgtcga gggcttgtat gccattggcg
    11461 aggtgagtta taccggctta cacggcgcta accgcatggc ctcgaattca ttgctggagt
    11521 gtctggtcta tggctggtcg gcggcagaag atatcaccag acgtatgcct gatgcccacg
    11581 gcgtcagtac gttaccgccg tgggatgaaa gccgcgttga gaaccctgac gaacgggtag
    11641 taattcagca taactggcac gagctacggc tgtttatgtg ggattacgtt ggcattgtgc
    11701 gcacaacgaa gcgcctggaa cgcgccctgc ggcggataac catgctccaa caagaaatag
    11761 acgaatatta cgcccatttc cgcgtctcaa ataatttgct ggagctgcgt aatctggtac
    11821 aggttgccga gttgattgtt cgctgcgcga tgatgcgcaa agaaagccgg gggctgcatt
    11881 ttacgctgga ttacccggaa ctgctcacgc attccggtcc atcgatcctt tcccccggca
    11941 atcattacat aaacagataa aaagcctggg tcagcgccgt atacgcttcg gaatagttct
    12001 ggtctggccc acgaatgact aagcgatcgc taaagcattc tcccgcctgc ggggagaatg
    12061 ccagcagcac ccgatgcggc agtcgcgctt cgttttccgc cacatccgtc cgcaaacgta
    12121 aatgccagcc catgcttaat gccagttcag taaaaccatt accaatctgc tctggcagca
    12181 ccacgcagaa aaatccctct tcggtaatgc actccgccgc acaggtcagc agcgatgggt
    12241 gatcaagcgt agtggtatag cgagcctgtt cccgttgagg tgtcgcgcac tctactccct
    12301 gctgatagta aggtgggtta ctgatgatta aatcgaagcg tactgtctgc tgtgtgatcc
    12361 actgctgaat atccgccgta tggacgttaa tccgctctgc ccacggggac tggttgatat
    12421 tttcctgcgc ctgcgcggca gcttcacttt ccagctcaac ggcatcaatt attacgctgt
    12481 cactggttcg ctgcgccagc atcagtgcca gcaacccgct acccgcgccg atatcaaggc
    12541 aacgtttcac cccagccacc ggtgcccatg cgcccagcaa aatgccatcc gttcccactt
    12601 tcatcgcaca gcgatcgtga gcaacaaaaa actgtttaaa agtaaatcca ttacgacgaa
    12661 gcacggatgt agactgtgac atgaaaataa aaccttgcag gaaaaacggc gatagcaccg
    12721 ggtgagaaca atacccgaga agcgatatcc atacaaacag atgaagattg cagccgtaac
    12781 gtctataatc agcgccccac acagaggtag aacatgactg taacgacttt ttccgaactt
    12841 gaactcgacg aaagcctgct ggaagccctc caggataaag gtttcactcg cccgaccgcc
    12901 attcaggctg ccgccattcc gcctgcgctc gatggccgtg atgtactcgg ttctgcgccg
    12961 acaggcaccg gtaaaacggc ggcgtatctg ctgccagcgt tgcagcacct gctcgatttc
    13021 ccgcgtaaga aatccggtcc gccgcgtatt ttgatcctca ccccaactcg cgagctggcg
    13081 atgcaggtgg ccgatcatgc ccgcgaactg gcgaaacata cgcatctgga tatcgccacc
    13141 atcaccggcg gcgtagccta tatgaaccac gcggaagtgt tcagcgaaaa tcaggacatc
    13201 gtggtcgcca cgaccggacg tctgctgcaa tacataaaag aagagaactt cgattgccgc
    13261 gcggttgaaa cgctgatcct cgacgaagca gaccgtatgc tggatatggg cttcgctcag
    13321 gatatcgaac atattgctgg cgaaacgcgc tggcgtaaac agaccctgct cttttcggca
    13381 acgctggaag gcgatgcgat tcaggacttt gccgagcgtc tgctggaaga tccggtggag
    13441 gtttctgcca atccgtccac ccgtgagcgc aaaaaaattc atcagtggta ttaccgcgcc
    13501 gatgacctgg aacataaaac cgcattgctg gtgcatctgt taaaacagcc ggaagcgacc
    13561 cgctcaattg tgtttgtgcg taagcgtgaa cgtgtgcatg agctggcaaa ctggctgcgc
    13621 gaagcgggca tcaacaactg ctatctcgaa ggtgagatgg tacaaggcaa gcgtaacgaa
    13681 gcgatcaagc gtttgaccga aggtcgcgta aacgtactgg ttgcaaccga tgttgccgcg
    13741 cgcggtatcg acattcctga cgtcagccac gtctttaact tcgatatgcc gcgcagtggc
    13801 gatacttatt tgcaccgtat cggacgtacc gcgcgcgccg gtcgtaaagg caccgcaatt
    13861 tcgctggtgg aagcccatga ccatttgctg ctgggcaagg ttggccgtta cattgaagag
    13921 ccgattaaag ctcgcgttat tgatgagtta cgcccgaaaa cgcgtgcgcc aagcgagaag
    13981 cagaccggca agccatcgaa gaaagtactg gcaaaacgcg ccgagaagaa aaaagctaaa
    14041 gagaaagaga aaccgcgtgt gaaaaaacgc catcgcgaca ccaaaaatat tggtaagcgc
    14101 cgtaaaccaa gcggaacggg cgtgccaccg caaacgacag aagagtaatc tcaatgccgg
    14161 gtttaagcct ggtattaaaa gtgctggc
//
LOCUS       DAZGLJ010000120        13953 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000120 DAZGLJ010000000
VERSION     DAZGLJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.120"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1119
                     /gene="yghO"
                     /locus_tag="ACHK49_003073"
     CDS             <1..1119
                     /gene="yghO"
                     /locus_tag="ACHK49_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO9263686.1"
                     /translation="KIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIERNEHLSAKN
                     PGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDDPQVFAALF
                     GAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYAAHIEQLGY
                     HKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQILREIFNSGW
                     QHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNINEAIADLNG
                     SLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEALRDPFARRK
                     IDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1126..1890)
                     /gene="glcC"
                     /locus_tag="ACHK49_003074"
     CDS             complement(1126..1890)
                     /gene="glcC"
                     /locus_tag="ACHK49_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO9263687.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2141..3640
                     /gene="glcD"
                     /locus_tag="ACHK49_003075"
     CDS             2141..3640
                     /gene="glcD"
                     /locus_tag="ACHK49_003075"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO9263688.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3640..4692
                     /gene="glcE"
                     /locus_tag="ACHK49_003076"
     CDS             3640..4692
                     /gene="glcE"
                     /locus_tag="ACHK49_003076"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO9263689.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAINEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4703..5926
                     /gene="glcF"
                     /locus_tag="ACHK49_003077"
     CDS             4703..5926
                     /gene="glcF"
                     /locus_tag="ACHK49_003077"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO9263690.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            5931..6335
                     /gene="glcG"
                     /locus_tag="ACHK49_003078"
     CDS             5931..6335
                     /gene="glcG"
                     /locus_tag="ACHK49_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO9263691.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6357..8528
                     /gene="glcB"
                     /locus_tag="ACHK49_003079"
     CDS             6357..8528
                     /gene="glcB"
                     /locus_tag="ACHK49_003079"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO9263692.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPECVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFQVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDAAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHAMHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8756..9001
                     /gene="traT"
                     /locus_tag="ACHK49_003080"
                     /pseudo
     CDS             8756..9001
                     /gene="traT"
                     /locus_tag="ACHK49_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            complement(9034..10776)
                     /gene="cptA"
                     /locus_tag="ACHK49_003081"
     CDS             complement(9034..10776)
                     /gene="cptA"
                     /locus_tag="ACHK49_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO9263693.1"
                     /translation="MQKTLTKHEHVFSWKALGWALIYFWYFSTLLQIIICLTGYSGTN
                     GLRDSLLFSSLWLIPVFLFPERIKVTAALIGVVLWGTSLAALCYYLIYGQEFSQSVLF
                     VMFESNTSEASEYFSQYFSLKILFISTIYTAIAILLWTRLSPIYIPSYWRYIIPLFII
                     YGLILHPVLINTVVKNRTIDNTLGRLASRMEPAAPWQFITGYYQYRLQLNSLNRLLEE
                     NNALPALKNFNDISGDTPRTLVLVIGESTQRGRMSLYGYPRKTTPGLDELYKTEQNMF
                     VFNNVVSSRPYTIEVLQQVLTFADEQQPELYLTQPSLMNIMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLAPFSEVLADPAPKKFVIIHLLGT
                     HIKYKYRYPEGQGVFDGDREHAPEGLTDNELELYNDYDNANIFNDHVVATLIKTFKAT
                     APDGFLFYFSDHGEEVFDTPPHKMLGRNEDTPTRPMYNIPFILWASEKWLLNRHHTFS
                     EYINRKYSLADFIHTWSDLAGLRYDNYEPERSILNQHFKEITRWIGNPYKKNALTDYD
                     TLPYAELRVNQASN"
     gene            complement(11195..11959)
                     /locus_tag="ACHK49_003082"
                     /pseudo
     CDS             complement(11195..11959)
                     /locus_tag="ACHK49_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            12179..12536
                     /locus_tag="ACHK49_003083"
                     /pseudo
     CDS             12179..12536
                     /locus_tag="ACHK49_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(12790..>13953)
                     /gene="iha"
                     /locus_tag="ACHK49_003084"
     CDS             complement(12790..>13953)
                     /gene="iha"
                     /locus_tag="ACHK49_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO9263694.1"
                     /translation="ENKGRELVRNVLKRDKWGLAGQLRELKESNLILNSLLLTPLGES
                     HLVTVGGEYQSSSMKDGVVLASTGETFRQKSWSVFAEDEWHLTDALALTAGSRYEHHE
                     QFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLHKGISGVSGQGKTNLLGNPD
                     LKPEESVSYEAGVYYDNPAGLNANITGFMTDFSNKIVSYSINDNTNSYINSGKARLHG
                     VEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSYTPEHMVNAKLNWQITEEVA
                     SWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAWTVVDAGLSWKVTDALTLNA
                     AVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYVIPERNYWMSLNYQF"
BASE COUNT         3462 a         3429 c         3738 g         3324 t
ORIGIN      
        1 aaaattgaaa aagtaattaa taaaaatgac cttaaggcat ttatcgcttt tccgtcatca
       61 ctttatcccg acgatccaaa ctggataccc cctttattca tagaacgcaa cgagcatttg
      121 tctgcgaaaa atccggggac ggaccatatc atctggcagg cgtgggtggc aaaaaaagcg
      181 gggcagatag ttgggcgtat taccgcgcaa atagatacct tgcaccgtga gcgttacggt
      241 gaagataccg gtcatttcgg catgattgac gccattgatg atccgcaggt ttttgccgcg
      301 ttgtttggcg cagcggaagc gtggttgaag tcacaaggtg caagtaagat cagcggtcct
      361 ttcagcctga atatcaatca ggaaagcgga ttactgattg aaggttttga cacaccaccc
      421 tgtgcgatga tgccacacgg caaaccgtgg tatgccgcgc atattgaaca actgggttat
      481 cacaaaggca ttgatttact ggcgtggtgg atgcagcgaa ccgatctcac tttctctccg
      541 gcgctaaaaa aactgatgga tcaggtgcgc aaaaaggtga ccattcgctg catcaatcgt
      601 cagcggtttg ccgaagaaat gcagatcctg cgtgagattt tcaactctgg ctggcagcac
      661 aactggggat ttgtgccgtt taccgaacat gaattcgcga ccatgggcga tcaacttaaa
      721 tatctggtgc cggacgatat gatctatatc gctgagattg attctgcacc ctgcgcgttt
      781 attgtcggct tgccgaacat caacgaggcg attgccgatc tgaacggatc gctctttccc
      841 ttcggctggg caaaattgct gtggcgtttg aaagtcagcg gtgtgcgaac tgcgcgagta
      901 ccgctgatgg gcgtgcgcga cgagtatcag ttcagccgca tcggcccggt gattgcgctg
      961 ttattgattg aagccttacg cgatccgttt gcccgccgga agatcgatgc gctggagatg
     1021 tcatggatcc tcgaaaccaa tacaggcatg aataacatgc tggaacgcat tggggcggag
     1081 ccgtacaagc gttaccgatt gtatgaaaag cagatttaat accagctaac tcaggttcat
     1141 ctccagcggc accgccgagc gaatcaaatg gtggccttcc agctcgatat cgtggagatt
     1201 ctttttcacg gtccgcacat gatcgcgtgc tgcgcgctgg gcgacgtgcg gcagccgctg
     1261 caacaccgcg ttgtagatcc tcgcatgctg gcgatcgatc tgcttttttt gctgtggtcg
     1321 atggtagaga ttatttacgc tggcaaacac tgaattaaac atcagatcgg tcaatgattg
     1381 cagcgtaaac accagcacct gattgtgaga agcctgacaa atggcgagat ggaaagcgtg
     1441 atccaactgc gcatgttcga tcagcgaaat ctctttgttg ttctcactgg cggcgagcat
     1501 tttttcataa cagcgggtta tcacaacaaa atcagcctgc gttcccagcg ttgccgccag
     1561 ccttgccgat tcgccctcca gtaatgcgcg aacgtcgagc agatcgtaca gcgttcgcgg
     1621 ctgcgtactg aacagatgga tcagcggact ggtgtcctgc acccgattaa gccgtgcgac
     1681 acgagaatcg cgaccctgcg ccgtttcaat aatcccgcgc ccgcgcagca cggtcagccc
     1741 ttcacgcagt gcggagcgtg agaagccgag cttttcacac agtcgacgtt ccgagggaag
     1801 tggctgaccg accttcagta cgccgtcgat aattaaccgt tcgatactct ctgcaaccac
     1861 ttcgcaaata gggcgacgtt catctttcat tcccggacct cgtgcacagg taggaccaat
     1921 ttttctgttc ttaactcgca aaacacgcac atcacgtaag tgattatgtt acatcaattt
     1981 aacattgagt taaccaaggc aaggtcacag agctggaaaa aaaatggtct gaccggtagc
     2041 taaagagata gacgaaaacg aaaagcccgc ttaataactg ttcacagaag cagcgcgcaa
     2101 aaatcagctg ccacacaaca caacaaagcg aagcctactc atgagcatct tgtacgaaga
     2161 gcgtcttgat ggcgctttac ccgatgtcga ccgcacatcg gtactgatgg cactgcgtga
     2221 gcatgtccct ggacttgaga tcctgcatac cgatgaggag atcattcctt acgagtgtga
     2281 cgggttgagc gcgtatcgca cgcgtccatt actggttgtt ctgcctaagc aaatggaaca
     2341 ggtgacagcg attcttgctg tctgccatcg cctgcgtgta ccggtggtga cccgtggtgc
     2401 aggcaccggg ctttctggtg gcgcgctgcc gctggaaaaa ggtgtgttgt tggtgatggc
     2461 gcgctttaaa gagatcctcg acattaaccc cgttggtcgc cgcgcgcgcg tgcagccagg
     2521 cgtgcgtaac ctggcgatct cccaggccgt tgcaccgcat aatctctact acgcaccgga
     2581 cccttcctca caaatcgcct gttccattgg cggcaacgtg gcagaaaatg ccggcggcgt
     2641 ccactgcctg aaatatggtc tgaccgtaca taacctgctg aaaattgaag tgcaaacgct
     2701 ggacggcgag gcgctgacgc tgggatcgga cgcgctggat tcacctggtt ttgacctgct
     2761 ggcgctgttc accggatcgg aaggtatgct cggcgtgacc accgaagtga cggtaaaact
     2821 gctgccgaag ccgcccgtgg cgcgggttct gttagccagc tttgactcgg tagaaaaagc
     2881 cggacttgca gttggtgaca tcatcgccaa tggcattatc cccggcgggc tggagatgat
     2941 ggataacctg tcgatccgcg cggcggaaga ttttattcat gccggttatc ccgtcgacgc
     3001 cgaagcgatt ttgttatgcg agctggacgg cgtggagtct gacgtacagg aagactgcga
     3061 gcgggttaac gacatcttgt tgaaagcggg cgcgactgac gtccgtctgg cacaggacga
     3121 agcagagcgc gtacgtttct gggccggtcg caaaaatgcg ttcccggcgg taggacgtat
     3181 ctccccggat tactactgca tggatggcac catcccgcgt cgcgccctgc ctggcgtact
     3241 ggaaggcatt gcccgtttat cgcagcaata tgatttacgt gttgccaacg tctttcatgc
     3301 cggagatggc aacatgcacc cgttaatcct tttcgatgcc aacgaacccg gtgaatttgc
     3361 ccgcgcggaa gagctgggtg ggaagatcct cgaactctgc gttgaagttg gcggcagcat
     3421 cagtggggaa catggcatcg ggcgagaaaa aatcaatcaa atgtgcgccc agttcaacag
     3481 cgatgaaatc acgaccttcc atgcggtcaa ggcggcgttt gaccccgatg gtttgctgaa
     3541 ccctgggaaa aacattccca cgctacaccg ctgtgctgaa tttggtgcca tgcatgtgca
     3601 tcacggtcat ttacctttcc ctgaactgga gcgtttctga tgctacgcga gtgtgattac
     3661 agccaggcgc tgctggagca ggtgaatcag gcaattagcg ataaaacgcc gctggtgatt
     3721 cagggcagca atagcaaagc ctttttaggt cgccctgtca ccgggcaaac gctggatgtg
     3781 cgttgtcatc gcggcattgt taattacgac ccgaccgagc tggtgataac cgcgcgtgcc
     3841 ggaacgccgc tggtggcgat tgaagcggcg ctggagagcg ccgggcaaat gctcccctgc
     3901 gagccgccgc attatggtga agaagccacc tggggcggga tggtcgcctg cgggctggcg
     3961 gggccgcgtc gcccgtggag cggttcggtc cgcgattttg tcctcggcac gcgcatcatt
     4021 accggcgctg gaaaacatct gcgttttggt ggcgaagtga tgaaaaacgt tgccggatac
     4081 gatctctcac ggttaatggc cggaagctac ggttgtcttg gcgtgctcac tgaaatctcg
     4141 atgaaagtgt taccgcgacc gcgcgcctcc ctgagcctgc gtcgggaaat cagcctgcaa
     4201 gaagccatta acgaaatcgc ccagtggcaa ctccagccgc tacccattag tggcttatgt
     4261 tacttcgaca atgcgttgtg gatccgcctt gagggcggcg aaggatcggt aaaagcggcg
     4321 cgtgaactgc tggggggcga agaggttgcg ggtcagttct ggcagcaatt gcgtgaacaa
     4381 caactgccgt tcttctcgtt accaggtacc ttatggcgca tttcattacc cagcgatgcg
     4441 ccgatgatgg atttacccgg cgagcaactg atcgactggg gcggagcgtt acgctggctg
     4501 aaatcgacag ccgaggacaa tcaaatccat cgcatcgccc gcaacgctgg cggtcatgcg
     4561 acccgcttta gtgccggaga tggtggcttt gccccgctat cggctccttt attccgctat
     4621 caccagcagc ttaaacagca gctcgaccct tgcggcgtgt ttaaccccgg tcgcatgtac
     4681 gcggaacttt gaggagcagg ctatgcaaac ccaattaact gaagagatgc ggcagaacgc
     4741 gcgcgcgctg gaagccgaca gcatcctgcg cgcctgtgtt cactgcggat tttgtaccgc
     4801 aacctgccca acctatcagc ttctgggcga tgaactggac gggccgcgcg ggcgcatcta
     4861 tctgattaaa caggtgctgg aaggcaacga agtcacgctt aaaacacagg agcatctcga
     4921 tcgctgcctc acttgccgca attgtgaaac cacctgtcct tctggtgtgc gctatcacaa
     4981 tttgctggat atcgggcgtg atattgtcga gcagaaagtg aaacgcccac tgccggagcg
     5041 aatgctgcgc gaaggattgc gccaggtagt gccgcgtccg gcggtcttcc gtgcgctgac
     5101 gcaggtaggg ctggtgctgc gaccgttttt accggaacag gtcagagcaa aactgcctgc
     5161 tgaaacggtg aaagctaaac cgcgtccgcc gctgcgccat aagcgtcggg ttttaatgct
     5221 ggaaggctgc gcccagccta cgctttcgcc caacaccaac gcggcaactg cgcgagtgct
     5281 ggatcgtctg gggatcagcg tcatgtcagc taacgaagca ggctgttgtg gcgcggtgga
     5341 ctatcatctt aatgcgcagg agaaagggct ggcacgggcg cgcaataata ttgatgcctg
     5401 gtggcccgcg attgaagcag gtgccgaggc aattttgcaa accgccagcg gttgcggcgc
     5461 gtttgtcaaa gagtacgggc agatgctgaa aaacgatgcg ttatatgccg ataaagcgcg
     5521 tcaagtcagt gaactggcgg tcgatttagt cgagcttctg cgcgaggaac cgctggaaaa
     5581 actggcagtt cgcggcgata aaaaactggc cttccactgc ccgtgtaccc tacaacatgc
     5641 gcaaaagctg aacggcgaag tggaaaaagt gttgcttcgt cttggattta ccttaacgga
     5701 cgttcccgac agccatctgt gctgcggttc agcgggaaca tatgcgttaa cgcatcccga
     5761 tctggcacgc cagctgcggg ataacaaaat gaatgcgctg gaaagcggca aaccggaaat
     5821 gatcgtcacc gccaacattg gttgccagac gcatctggcg agcgccggtc gtacctcagt
     5881 gcgtcactgg attgaaattg tagaacaagc ccttgaaaag gaataaaaaa atgaaaacta
     5941 aagtcattct tagccagcaa atggcgagtg caattattgc cgcaggtcag gaagaggcgc
     6001 agaaaaataa ctggtcggtt tccattgctg ttgccgatga cggcggtcat ctgctggcgt
     6061 taagtcgcat ggacgattgc gcgccgattg cggcttacat ctcccaggag aaagcgcgta
     6121 ccgccgcgct ggggcgtcgt gaaactaagg gctatgaaga gatggtgaac aacggacgta
     6181 ccgcgttcgt gactgcgccg ttactgacgt cgctggaagg cggcgtaccg gttgttgtgg
     6241 atgggcaaat tattggtgcc gtgggcgttt ctggtttaac cggagcacag gatgcgcagg
     6301 tcgcgaaagc ggcagcagcg gtgttggcga aataagcgaa aacgaggaga gaaacaatga
     6361 gtcaaaccat aacccagggc cgtttacgca ttgacgccaa ttttaaacgt tttgtggatg
     6421 aagaagtttt accgggaaca gggctggacg ctgcggcgtt ctggcgcaat tttgatgaga
     6481 tcgttcacga tctggcacca gaaaatcgtc agttgctggc agaacgcgat cgcattcagg
     6541 cagcgcttga tgagtggcat cgcagcaatc cggggccggt aaaagataaa gcggcctata
     6601 aatctttcct gcgtgaactg ggctacctgg tgccgcaacc ggagtgcgtg acggtggaaa
     6661 ccacgggcat tgacagcgaa atcaccagtc aggcagggcc acagctggtg gttccggcaa
     6721 tgaacgcccg ctacgcgctg aacgcggcga acgctcgctg gggctcactg tacgatgcgt
     6781 tatacggcag cgacatcatc ccgcaggaag gggcgatggt cagcggctac gatccgcagc
     6841 gcggtgagca ggttatcgcc tgggttcggc gtttcctcga tgaatctcta ccgctggaaa
     6901 acggcagcta tcaggatgtg gtggcgtttc aggtggtcga taaacaatta cgcatccagt
     6961 tgaaaaatgg taaagaaacc acgttacgta ccccggcgca gtttgtcggt taccgtggcg
     7021 atgccgctgc gccgacctgc attttgctga aaaataacgg cctgcatatt gaactgcaaa
     7081 ttgatgccaa cggacggatt gggaaagacg atgcggcgca catcaacgat gttatcgtcg
     7141 aagctgcaat cagtaccatt ctcgactgcg aagattcggt cgcggcggtt gatgcggaag
     7201 ataaaatcct gctgtaccgc aatctgctgg gcctgatgca ggggactctc caagagaaaa
     7261 tggagaaaaa cggtcgacaa atcgtgcgta aactgaatga cgatcgtcag tacaccgccg
     7321 ccgatggctc tgaaatttct ctgcacggac gctcgctgct gtttatccgc aacgtgggtc
     7381 atttgatgac cattcctgtg atttgggaca gcgaaggcaa tgaaatcccg gaaggcattc
     7441 ttgatggcgt catgactggc gcgattgccc tctatgattt aaaagtgcag aaaaactcgc
     7501 gcactggcag cgtctatatt gtgaaaccga aaatgcacgg tccgcaggaa gtggcgttcg
     7561 ccaacaaact gtttacccgc attgagacaa tgctcggtat ggcaccgaat accctgaaaa
     7621 tgggcattat ggatgaagaa cgtcggacct cgctgaactt gcgtagctgt atcgctcagg
     7681 cacgcaaccg cgtggcgttc atcaataccg gtttcctcga ccgtaccggc gatgaaatgc
     7741 attcggtgat ggaagctggc ccgatgctgc gtaaaaatca gatgaaatcg acgccgtgga
     7801 tcaaagccta cgagcgtaat aacgtgcttt ccggcctgtt ctgtgggctg cgcggtaaag
     7861 cgcaaatcgg taaaggcatg tgggcaatgc cggacctgat ggcagacatg tacagccaga
     7921 agggcgacca actgcgtgcc ggggcaaaca cagcctgggt tccgtcgcca actgccgcta
     7981 cgctccatgc gatgcactac caccaaacca acgtacagag cgtgcaagcc aacattgccc
     8041 agactgagtt caatgctgaa tttgaaccgc tgctggacga tctgctgact attccggttg
     8101 ctgaaaacgc taactggtcg gcgcaagaga tccaacaaga gctggataac aacgtgcagg
     8161 ggattctggg gtacgtggtg cgctgggtgg agcaggggat tggttgttca aaagtgccgg
     8221 atattcacaa tgtggcgctg atggaagacc gcgcaacgct gcgtatctcc agccagcata
     8281 tcgccaactg gttacgtcac ggtattctga ccaaagaaca ggtgcaggcg tcgctggaga
     8341 atatggcgaa agtggttgat cagcaaaacg ctgacgatcc ggcttatcgt ccgatggcgg
     8401 ggaatttcgc taactcgtgt gcttttaaag ctgccagcga tttaatcttc ctcggcgtga
     8461 aacagccaaa cggctatacc gaaccgttat tacacgcctg gcgtttacgc gaaaaagaaa
     8521 gtcattaata gcgtcaaaaa aagccccggc aagttttatt gtcggggcta tgatagtaac
     8581 tgcggtagta catttatccc ggaaataacc ggagcgatta tcagggttta acaatattaa
     8641 atacccgtag cgcattacta gcgggcctat aacaacaata tggacatctt aacgatactc
     8701 tgcgcctgcg tgtgcagaac agaaaccctt acccgatata cttgttatgc cagaggtagc
     8761 aattgcggca accgatctga ctggcatgga tgcagatgca atggtggaag atataaatta
     8821 cacgataatt acagatgttc agattaccga acgaactaaa acctatgtca gagcagataa
     8881 tgctaccact tttcggcagg gaacatcagg caccgaaatt caggctagca cagaaaccag
     8941 caaccaggtt aatctgaaat ttgaaggggc taaacctctt ctggaaatac aactgaaata
     9001 aatatcctcc ggctatgccg gaggatattt attttaattt gaagcctgat ttacacgcag
     9061 ctctgcgtag ggcagggtat catagtcagt taacgcattt ttcttatagg ggtttcctat
     9121 ccagcgagtg atttctttga aatgctggtt taatatggaa cgctctggct catagttatc
     9181 ataacgtaaa ccagccaaat ccgaccaggt atggataaaa tcagccagac tgtatttacg
     9241 gttaatgtac tcagaaaatg tatgatgtcg attaagtagc catttttcag aggcccagag
     9301 aataaatgga atattgtaca taggtcgggt tggggtgtct tcgttacgtc ccagcatttt
     9361 atgtggtggc gtatcgaata cttcctctcc atggtcagaa aaatagaaaa gaaaaccatc
     9421 aggggctgtt gctttgaaag ttttgatcaa cgttgcaaca acatggtcat taaatatatt
     9481 agcattatcg taatcgttat ataattccag ttcattatca gtaagtcctt ctggtgcatg
     9541 ttccctgtcc ccatcgaata ctccctgacc ttcaggataa cggtatttat atttgatgtg
     9601 tgttcccaac aagtgaataa taacaaattt tttgggagct ggatctgcca gtacttcact
     9661 aaacggagcc agtacgttag tatcgtactc acgggcactt tgcgttctct gctgattcat
     9721 gtagtactgt ttatctgttt gccgagagaa gactgtcagc attgtgttgc gtgcagtcat
     9781 cgtttgttga ttggtgatcc agaatgtttt gtaaccggcc tgtttcatta tattcatcag
     9841 ggaaggttgt gtcaggtaga gttcgggctg ctgttcatca gcgaaagtca atacctgctg
     9901 tagtacttcg atggtatatg gtcttgagga gacaacatta ttaaacacaa acatattttg
     9961 ttccgttttg tataattcat ctagtccggg ggttgttttt ctgggatatc catacagact
    10021 cattctccca cgctgggttg attctcctat caccagaacc agtgttcgtg gggtatctcc
    10081 ggaaatatcg ttaaagtttt tcagagcggg gagcgcatta ttttcctcca gcaaacggtt
    10141 cagagaatta agctggagac ggtactgata atatccggta ataaattgcc agggggcagc
    10201 gggctccata cgggatgcca ggcgtcccag agtattgtca atagtcctgt ttttaacaac
    10261 agtatttatt aatacagggt gcagaattaa accgtagata ataaataaag gtataatgta
    10321 gcgccagtat gatggtatat atatcgggct aagacgagtc catagcagaa tagcaatagc
    10381 tgtatatatt gtcgatataa atagtatttt taaactaaaa tactgactaa aatattcgct
    10441 ggcttcactt gtattacttt caaacatgac gaacagtaca ctctgagaga attcctgccc
    10501 gtagattaga taatagcaaa gggcagcaag agatgtgccc cataaaacca cgccgattaa
    10561 tgctgctgta acctttattc tctctgggaa aagaaaaaca gggattaacc agagagagct
    10621 gaaaagtagc gagtcacgaa gtccattggt gccactatat ccagtcagac aaatgataat
    10681 ctgaagtaaa gtggaaaaat accagaaata aatgagagcc catcccaggg ctttccagct
    10741 aaaaacatgt tcatgttttg ttaatgtctt ctgcatatta ataaacctgc acgggaattt
    10801 actattgtaa tttttatatg agatgtatta aaacatttca tacagatttt ttgataagca
    10861 catagtttca atattcacta taatcgtttt ctgctgtgtt gtgtttgatc ctttttcaaa
    10921 gaggcgctaa tcttccactt gccaaggtta gatggtcagg gtccgggtta aacaaaatgt
    10981 ttgacaggga gcctttccat atgctgtctt tccagcgtgg cggtccgtaa tgtactaatt
    11041 ttaagagagg tccgcaatct tccagtggat ggttcatctg cctctctatt accgttacca
    11101 tcgtcttgcg tcctgtgcta tcgcctgtaa cgacatcagc gtcactggta ttaacaaact
    11161 tatagttgaa gccagtcact tcgtctcttg gctaacgcag cgtgatgagc tggagtgcca
    11221 ggcgtgggag aacaacgaca gcagcattcg taccggggac gatgacggtt tgctgtgtca
    11281 gtgcctgggg gatatctgca gcaaggcgtt ttcccagacg gggcgcgagt ttgcagagct
    11341 gggttgccag ttttttctca cccagtaaag cgagtttttc gggtgaggga tatcgctgga
    11401 gaagatcaag tactgcagga tgatccagtc gcgggccgag aacgcgctcc agagccggat
    11461 gtatctgggt caacaagccg cggatgcggt tgctggcctg tgttgtctgt gcggcaagat
    11521 catcatcgaa gccgcaaagc atggagagtt cggcgatttg ttcgtcggcc agttttagag
    11581 agcgaagagc atgaggcaga gtacgtgcag cttcggcaat gatggccgca tcacgggcat
    11641 cggttttggc ttcaccggag tgcaaatcgg cgatgcgtcg cattgccagt ccaggaaggt
    11701 atccgacaag gacgccttca gagcgggcaa cggcgacggg taacgccctg atggtagcag
    11761 gctggtcaac gaccagcagt atctgtccat gctgtttcag gtcagatatc agcgctctga
    11821 gtttgttttc gtcgttgggt aatactctgt cgaacaaacg tttacctgaa cgattaacgg
    11881 caacggcgta atgggtgtct ttacctacat caacgccaat aaagaccagg attgattcgt
    11941 aatcgctgga ttcgggcatt gtgtctcccc gtttatacgg gttaaccaga taaccttggg
    12001 gagcaggtac cggcatccac gttacagact gtcccgacat gatgtctgac caggccccta
    12061 tcagcggtta ccagcgcccc accagacccg gtgacatccc cccgaatcat ggacgactgg
    12121 gggaattaat catgccgggg ctggctaaca ccccattaca agggtacgaa taaagtaacg
    12181 gggagttaca ggcactgaaa ctatcatcag agatactgtt gccaaatggc gtaaaggttg
    12241 gatcccgccg tttgctattg ccgcccgact tccttcactg tccctgggaa gctgcggggt
    12301 ggtgccctgg aaaataatca gggggaagaa aattatgcgt cccgatttgt gaaacagaac
    12361 cggaacagaa aatagcgcag caactggcac tcgagttcta ccgtattctg ccccccaaat
    12421 aaatcacagc taaacagttg gttcactcaa gtccacgaaa gcggttcact agaatttcag
    12481 cgcgtagcca cggggatgga gccgatactg cagctatatg tgagacaatt gtcagcgcca
    12541 gtgatataag acggtaatgg agatgcggac gcggccctta attcagagct agccgagtat
    12601 aaatttctgg acaagtatgt aaatgatgag ttaaaaaata atccgtgcat ttccggtgat
    12661 gatattagtt gttggtaacg aaatctcatt agcggaccgg aaacgcactt aacctaaaac
    12721 attgccaccg ttccgccagg agttgcagtg gcaacgtatg ctaccgtcag tgatagcgtt
    12781 ttgttgttat cagaactgat agttcagcga catccagtaa tttcgctcag gtatcacata
    12841 tcccgttgtt gatgatcccg tctggaagta atcaccggca taaagcgtac tcttaccggc
    12901 actgtacaga ctcacgtcac tgtaatcctt gttgagcagg ttattcactg ccgcattcag
    12961 cgtcagggca tccgtcacct tccacgacag acctgcatcc accaccgtcc aggctttcag
    13021 gtattctcct ttctcatcat atactttctt ctgtacagcg ctcagcgacg aataattctg
    13081 ggtgaaacgt ggtgttttcc cgcgataacg ggcacccagc catgatgcca cctcttcggt
    13141 gatctgccag ttcagtttcg cattcaccat gtgttctggg gtataactca gcggtgcacc
    13201 tttattatca ccatcacgtt gttcacttcg ggtccaggtg taattcagcg acagcgtgac
    13261 atcctctgac catagcggca atgtgccggc aaattccaca ccgtgcaacc gggcctttcc
    13321 gctgtttata tagctattgg tgttatcatt tatggaataa gagacaatct tgttagagaa
    13381 gtcagtcata aaacctgtga tattggcgtt cagaccggcg gggttatcgt aatacacccc
    13441 agcctcataa ctgacgctct cttccggctt caggtcgggg ttaccaagta gatttgtttt
    13501 tccctgcccg gacacaccac taatcccttt atggagctgc cccattctgg gtgccttata
    13561 tcccgtggtc acaccgcctt tcagcgtcca ggcatctgcc acatcccaga ccagatatgc
    13621 acgcggactg aagtgccccc cgaattgctc atgatgttca tagcggctgc ccgcagtcag
    13681 cgcaagtgca tccgtgagat gccactcgtc ctcagcaaat accgaccagc ttttctgccg
    13741 gaaagtttca cctgtgctgg caaggacaac cccatctttc atggacgaac tctgatactc
    13801 gccccccaca gtaaccagat gagattctcc cagaggggta agcagcaatg aattcaggat
    13861 aaggttagat tccttaagct cccgcagttg accggcaagg ccccatttgt cgcgtttcag
    13921 tacattgcgt acaagctcac gacctttatt ttc
//
LOCUS       DAZGLJ010000121        13870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000121 DAZGLJ010000000
VERSION     DAZGLJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.121"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..165
                     /gene="kil"
                     /locus_tag="ACHK49_003085"
     CDS             <1..165
                     /gene="kil"
                     /locus_tag="ACHK49_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO9263695.1"
                     /translation="DYVLHEGRTYIASANNIKKRKLYIRNLTTKTCISDCMIKVFLGR
                     DGLPVKAESW"
     gene            165..335
                     /locus_tag="ACHK49_003086"
     CDS             165..335
                     /locus_tag="ACHK49_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFO9263696.1"
                     /translation="MTKKIKCAYHLCNKGIEESKSIKRPLHFMRGVIPTTEMKKYCSE
                     NCAEKDQMAHEL"
     gene            409..684
                     /locus_tag="ACHK49_003087"
     CDS             409..684
                     /locus_tag="ACHK49_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263697.1"
                     /translation="MITNYEVTVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVTKMSLVHIGKNLPAEKKAGFLATLIAMKLKGEI"
     gene            783..3434
                     /gene="recE"
                     /locus_tag="ACHK49_003088"
     CDS             783..3434
                     /gene="recE"
                     /locus_tag="ACHK49_003088"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFO9263698.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTCATLDYLIVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQE
                     DAPETEEPTGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDRKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRSMAEPTTLDNAALLRLTDAIKAVFSITKKHQ
                     PYEFKNFISAWLDTEHIDRGLLTKEWRKGNRVSRITRTASGANAGGGNLTDRGEGFVH
                     DLTSLARDVATGVLARSMDVDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGL
                     DYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMP
                     QRVTEEGKQDDEEKPQPSGAMADEQATAETVEPNATEHHQNTQPLDAQSQVNSVDAKY
                     QELRAELHEARKNIPSKNPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDSVYQN
                     QPETEKISPDAKQPEPVVQQEPEIVCNACGQTGGDNCPDCGAVMGDATYQETFGEENQ
                     VEAKEKDPEEMEGAEHPHNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNEN
                     YHAGPGVSKSQLDDIADTPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRF
                     IVAPEFNRRTNAGKEEEKAFLMECASTGKTVITAEEGRKIELMYQRVMALPLGQWLVE
                     SAGHAESSIYWEDPETGILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQ
                     DAFYSDGYEAQFGVLPTFVFLVASTTVECGRYPVEIFMMGEEAKLAGQQEYHRNLRTL
                     ADCLNTDEWPAIKTLSLPRWAKEYAND"
     gene            3427..4236
                     /gene="recT"
                     /locus_tag="ACHK49_003089"
     CDS             3427..4236
                     /gene="recT"
                     /locus_tag="ACHK49_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFO9263699.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            4293..4487
                     /gene="ralR"
                     /locus_tag="ACHK49_003090"
     CDS             4293..4487
                     /gene="ralR"
                     /locus_tag="ACHK49_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFO9263700.1"
                     /translation="MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALERWNKRTTGNNNGGVHV"
     gene            4480..4668
                     /locus_tag="ACHK49_003091"
     CDS             4480..4668
                     /locus_tag="ACHK49_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO9263701.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLLSS"
     gene            4775..5056
                     /locus_tag="ACHK49_003092"
     CDS             4775..5056
                     /locus_tag="ACHK49_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO9263702.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLLEKLKHAAEKIRSQFT"
     gene            5022..6098
                     /locus_tag="ACHK49_003093"
     CDS             5022..6098
                     /locus_tag="ACHK49_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9263703.1"
                     /translation="MQRKKYDPNLPKNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRVN
                     QLETIKEKLGGVLLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPTWFPLAMDLALVTGQRREDITN
                     MRFSEIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGTVVEQCRMVSRGDYLISAGI
                     RKNSPDGSLHPDGLTKKFVAARKLTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(6491..6832)
                     /gene="yebY"
                     /locus_tag="ACHK49_003094"
     CDS             complement(6491..6832)
                     /gene="yebY"
                     /locus_tag="ACHK49_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO9263704.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(6845..7717)
                     /gene="copD"
                     /locus_tag="ACHK49_003095"
     CDS             complement(6845..7717)
                     /gene="copD"
                     /locus_tag="ACHK49_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO9263705.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(7721..8095)
                     /gene="yobA"
                     /locus_tag="ACHK49_003096"
     CDS             complement(7721..8095)
                     /gene="yobA"
                     /locus_tag="ACHK49_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO9263706.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            8234..8464
                     /gene="holE"
                     /locus_tag="ACHK49_003097"
     CDS             8234..8464
                     /gene="holE"
                     /locus_tag="ACHK49_003097"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO9263707.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            8566..9222
                     /gene="yobB"
                     /locus_tag="ACHK49_003098"
     CDS             8566..9222
                     /gene="yobB"
                     /locus_tag="ACHK49_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO9263708.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            9246..9908
                     /gene="exoX"
                     /locus_tag="ACHK49_003099"
     CDS             9246..9908
                     /gene="exoX"
                     /locus_tag="ACHK49_003099"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO9263709.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(9905..11965)
                     /gene="ptrB"
                     /locus_tag="ACHK49_003100"
     CDS             complement(9905..11965)
                     /gene="ptrB"
                     /locus_tag="ACHK49_003100"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO9263710.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCIDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(12174..12833)
                     /gene="yebE"
                     /locus_tag="ACHK49_003101"
     CDS             complement(12174..12833)
                     /gene="yebE"
                     /locus_tag="ACHK49_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO9263711.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(13160..13516)
                     /gene="yebF"
                     /locus_tag="ACHK49_003102"
     CDS             complement(13160..13516)
                     /gene="yebF"
                     /locus_tag="ACHK49_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO9263712.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(13583..>13870)
                     /gene="yebG"
                     /locus_tag="ACHK49_003103"
     CDS             complement(13583..>13870)
                     /gene="yebG"
                     /locus_tag="ACHK49_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO9263713.1"
                     /translation="AVEVKYVVIREGEEKMSFTSKKEADAYDKMLDSADLLDTWLTNS
                     PVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
BASE COUNT         3901 a         3294 c         3316 g         3359 t
ORIGIN      
        1 gattatgttc ttcatgaagg ccggacatat atcgcctcgg caaacaatat taaaaagcga
       61 aaactttata ttcgtaacct gactacaaaa acatgcattt ctgactgcat gattaaagtc
      121 ttcctcggtc gtgatggttt acctgtaaag gcggagtcat ggtgatgact aagaaaataa
      181 aatgtgctta ccacctttgc aataaaggaa ttgaagaaag caaaagcatt aaaagaccac
      241 ttcatttcat gcgtggagtt atcccaacga cggaaatgaa aaaatattgt agtgaaaatt
      301 gtgccgaaaa agaccagatg gcacacgaac tttaattaac tgactatgcg aaactgaatt
      361 tatgccagca atggcaggga ttcgctcaac cttaattaag gagaaaaaat gattaccaat
      421 tatgaagtca ctgttgtaac taccgatgac attgttcacg aggttaatct ggaaggaaag
      481 cgtattggct acgtgattaa aacagaaaat aaagaaaccc cattcactgt ggttgatatt
      541 gacggtccat caggcaacgt aaaaacactt gatgaaggtg tcacaaaaat gagtctggtt
      601 cacatcggaa agaatctgcc cgcagaaaaa aaagccggat ttctggcaac tctgattgca
      661 atgaaattaa aaggtgaaat ctgaaaaaag aaagcctgca caacgtgcag gcctgagtga
      721 agaacctggg acatttattc atcactcgca gtaattttaa tctgagttga ggttaaaaaa
      781 caatgagcac aaaaccactc ttccttttac ggaaagcgaa aaaatcatcc ggtgaacctg
      841 acgtcgtcct gtgggcaagc gacgattttg aatcaacctg tgccactctg gactacctga
      901 tcgttaagtc aggtaaaaaa ctgagcaact attttaaagc tgttgccaca aattttcctg
      961 tcgttaatga ccttccccct gaaggtgaga tcgattttac ctggagtgaa cgctatcaac
     1021 tcagcaaaga ctccatgacc tgggaactaa aaccgggagc agcgccagac gacgttcacc
     1081 atcaggagga tgcaccggaa accgaagaac cgacgggagg ccaggaagaa aacgcgcagg
     1141 cagacgccca cgaggattgc caggattgcg aagtctctgt agccactttg cggttcacac
     1201 agcgtcttct gcacattttt acgtatgcag ccggggatcg aaaatacctg catcatgcca
     1261 cccgtgaaca acgcaaacac attactgctc ttgagatgga tcaggaaaac agctatgtcc
     1321 agaatctgct gttggccata cgcagcatgg cagaaccgac aactctggat aatgccgccc
     1381 tgctccgcct gactgatgca attaaggcag tgttctctat cacgaaaaaa catcagccct
     1441 atgaatttaa gaatttcatt tcagcctggc tggataccga acacattgat cgcggtcttc
     1501 tgacaaaaga atggcgaaaa gggaatcgtg tttcacgcat cactcgcacg gcttccggtg
     1561 ctaatgctgg cggcgggaac ctcaccgatc gcggcgaagg tttcgtccac gatctgacat
     1621 cactggcgcg cgatgtagcc actggcgtac tggcccgttc aatggacgtg gacatctata
     1681 accttcatcc ggcacacgct aaacgcattg aggaaattat cgctgaaaat aaaccaccct
     1741 tttctgtttt ccgcgacaaa ttcatcacca tgcctggcgg gctggattat tcccgcgcca
     1801 tcgtggttgc gtccgtgaaa gaagcaccaa ttgggatcga ggtcatcccc gcacacgtca
     1861 ctgaatatct gaacaaagta ctgactgaaa ccgatcatgc caaccctgat ccggaaatcg
     1921 tggatattgc ctgcggtcgc tcctcggccc cgatgccgca acgagtaaca gaagaaggaa
     1981 aacaggacga tgaagaaaaa ccacaaccat ctggcgcaat ggcagatgaa caggcaacgg
     2041 ctgaaacagt ggaaccgaat gcaactgaac atcatcagaa cacgcagccg ctggatgctc
     2101 agtcacaggt aaattctgtt gatgcgaaat atcaggaact gcgggcagaa ctccatgaag
     2161 cccggaaaaa cattccatca aaaaatcctg tcgatgccga taaattgctt gctgcatcac
     2221 gtggtgaatt tgttgacgga attagcgacc cgaacgatcc gaaatgggtt aaggggatcc
     2281 agactcgcga ttctgtgtac cagaaccagc cagaaacgga aaaaatcagc ccggatgcga
     2341 aacaacctga gccagtagtg caacaggaac cggaaatagt ctgcaatgcc tgcggtcaga
     2401 ctggcgggga taactgccct gactgtggtg cggtgatggg cgacgcaaca taccaggaaa
     2461 cattcggtga agagaatcag gttgaagcta aggaaaaaga tccggaggaa atggaaggcg
     2521 ctgaacatcc gcacaatgag aatgctggca gcgatccgca tcgcgattgc agtgatgaaa
     2581 ctggcgaagt cgcagatccc gtaatcgtag aagacataga gccaggtatt tattacggaa
     2641 tttcgaatga gaattaccac gcgggtcccg gcgtcagtaa gtctcagctc gatgacattg
     2701 ctgatactcc ggcactgtat ttgtggcgta aaaatgcccc cgtggacact acaaagacaa
     2761 aaacgctcga tttaggaacc gctttccact gccgggtact tgaaccggaa gaattcagta
     2821 accgctttat cgtagcacct gaatttaacc gccgtacaaa cgccggaaaa gaagaagaga
     2881 aagcgtttct gatggaatgc gcaagcacag gaaaaacggt tatcactgcg gaagaaggcc
     2941 ggaaaattga actcatgtat caacgcgtta tggctttgcc gctggggcaa tggcttgttg
     3001 aaagcgccgg acacgctgaa tcatcaattt actgggaaga tccggaaaca ggaattttgt
     3061 gtcggtgccg tccggacaaa attattcctg aatttcactg gatcatggac gtgaaaacca
     3121 cagcggatat tcaacgattc aaaacggctt attacgacta ccgctatcac gttcaggatg
     3181 cattctacag tgacggttat gaagcacagt ttggcgtgct gccaactttc gtttttctgg
     3241 ttgccagcac aactgttgaa tgcggacgtt acccggttga gattttcatg atgggcgaag
     3301 aagcaaaact ggcaggccag caggaatatc accgcaatct gaggaccctg gctgactgcc
     3361 tgaataccga tgaatggcca gctattaaaa cgttatcact gccccgctgg gctaaggagt
     3421 atgcaaatga ctaagcaacc acctatcgca aaagccgatc tgcaaaaaac ccagggaaac
     3481 cgtgcaccag cagcagtaaa aaataacgac gtgatcagct ttattaatca gccatcaatg
     3541 aaagagcaac tggcagcagc tctcccacgc catatgacgg ctgaacgaat gatacgtatc
     3601 gccaccacag aaattcgtaa ggttccggcg ctaggaaact gtgacaccat gagttttgtc
     3661 agtgcgatcg ttcagtgttc acagctcggc cttgagccag gtagcgccct cggccacgca
     3721 tatttactgc cttttggtaa taaaaacgaa aagagcggta aaaagaacgt tcagctaatc
     3781 attggttatc gcggcatgat tgatctggct cgccgttctg gtcaaatcgc cagcctgtca
     3841 gcccgtgttg tccgtgaagg tgacgagttt agttttgaat ttggccttga tgaaaagtta
     3901 atacaccgcc cgggagaaaa cgaagatgca ccagtgaccc acgtctatgc tgtcgcaaga
     3961 ctgaaagacg gagggactca gtttgaagtt atgacgcgca aacagattga actggtgcgc
     4021 agccagagta aggctggtaa taacgggcca tgggtaactc actgggaaga aatggcaaaa
     4081 aaaacggcta ttcgtcgcct gtttaaatac ctgcctgtct caattgaaat ccagcgtgca
     4141 gtatcaatgg atgaaaagga accactgaca atcgatccgg cagactcctc tgtattaacc
     4201 ggggaataca gtgtaatcga taattcagaa gaataattca gcctggcggt gtaatgcacc
     4261 gccaacgtga gacagttttt atgacaaaaa ttatgagata tgacgatgtt aaaccatgtc
     4321 cgttttgtgg ttgtccatct gttacagtga aagcaatttc aggatattac cgggcaaaat
     4381 gcaacggatg cgaatcccga actggctatg gtggaagtga aaaagaagca ctcgaaagat
     4441 ggaataaacg aactactgga aataataatg gaggtgttca tgtataaaat aactgccact
     4501 attgaaaaag aaggtggcac tcctactaac tggacaagat attcaaaatc taaattaacg
     4561 aaatcagaat gcgaaaaaat gctctcaggg aaaaaagaag caggcgtgtc cagagagcag
     4621 aaagtaaagc tgataaattt taattgcgag aaacttctgt cctcgtgagt tgcattatat
     4681 acaaattaga acttcatagc tgattattaa aaatcaacca cacccgccag tattctgtat
     4741 atttactggc ggtcatatcg taagaggtat ggcaatgaat cttgtgacac tcaaaacgtg
     4801 gggaaaactc agatatccgg ataacccacc atcaatatca acgctgagac gatgggcaag
     4861 gaatggaaac atttatcctg cacctgaact acacgggagg agttacaggg tggttccgga
     4921 agctttctat atcaacccaa ataaggttga taccgatata acacaccatc agcctaatgg
     4981 gcgacaaggg agagacagtc cgttactgga gaagttaaaa catgcagcgg aaaaaatacg
     5041 atcccaattt acctaaaaac ttaacatatc gaaggaggga caaagcatat tactggcgca
     5101 accctctgac gaaagaagaa tttacactag gtaaaatttc aagaagagat gcagtagcgc
     5161 aggcaattga agcaaatcat tatatataca aaaactactc tcctgctgcc ttaattgaaa
     5221 aactaaaagg gttcgactca tttactatgg cagactggat tgaacgttac aaaacgattc
     5281 ttataaggag aaaagtgtcc agaaatactt ataaaattcg ggtaaatcaa ctggagacaa
     5341 taaaagaaaa attaggaggg gttttactga cagaaataac cactcgccat attgccgagt
     5401 ttcttgattt gtggattgaa ggagggaaaa acacgatggc aggatcaatg cgttctgtgt
     5461 tatctgatat gttccgcgag gccattgttg aaggacgtat atctcaaaat ccagtaacgc
     5521 caacaagagc accgaaaata gtagttacaa gagaacggct gaaactaaag acatacaact
     5581 gcatcaggga ggcagcagat caacttccga catggttccc attagctatg gacttagccc
     5641 ttgtaacagg acaacgtcgc gaagacataa caaatatgcg gttcagtgag atttatgatg
     5701 atcgtctcca catcaggcaa attaagacag gaatgatgat tgctatcccc ctgtcactca
     5761 gccttcctgt cgctggttta cgacttggta cagtagttga acagtgccgc atggtaagta
     5821 ggggggatta tctaatcagt gccgggatta gaaaaaacag ccctgacggc agccttcacc
     5881 cggacggcct gacaaagaaa tttgtcgcag ccagaaaatt aacaggtatc cagttcagtg
     5941 aaaacccacc aacttttcac gagatcagaa gcctggctgg acgattgtac aaagaaacat
     6001 gtggggaaga atttgctcaa cgtctacttg gccacacatc ggagaagaca acaaaaatgt
     6061 atcttgatga gagagaaaaa acgtacttac tgctctgatt ttaacgtaaa tggattgtta
     6121 aatgtgtttt ggttgtgata taaccaaaaa agaccggaat acagaaattc gagtaaattt
     6181 cgagtaattt cggggagacg tttgcaacta attgatttta aatacaatta aaaaaagacc
     6241 gaatacgatt cctgtattcg gtccagggaa atggctcttg ggagagagcc gtgcgctaaa
     6301 agttggcatt aatgcaggct tagttgcctt gccctttaag aatagatgac gacgccaggt
     6361 tttccagttt gcgtgcaaaa tggtcaataa aaagcgtggt ggtcatcagc tgaaatgtta
     6421 aaaaccgccc gttctggtga aagaactgag gcggtttttt tattggaaat caaaaggcta
     6481 ttttaggtaa ttaacagagt ttttcagctc gttctataaa cggtgccaga ctcatttttt
     6541 cgccgggatt gttaggatca tcaatctgaa tcaccgaaat gggttgggct ttagtcttcc
     6601 cactggcaac ttccttttgt gcgatatcgt ttaaaggata ctgcacgagg gtacttgggt
     6661 taatgacata caaagcatta cccggtctgc aagtcagcat cacctcttcg cgattaaacg
     6721 cccatttgtc tttacccact tcaaaacgac tgacggtaat cacctgcggt gcagccagcg
     6781 ccgctgcaga actggtgagt aacagaaacg tcagaatact ttttttcatc attttcgtaa
     6841 tccatcaaaa aggttcgaga gtcgcaagca ggctgatgat caccagcact accgcaccta
     6901 tcgcccattc gagtttagtc atccactcaa accagggtgc cgcacgatct tcatcttgcc
     6961 tcatgcgcgg tacaagaaca taacgattag ccagcgcaat gaccaccata atcatcacca
     7021 ggatggcttt gagcaataag agttggcccc agtaagtcgt aagcgtgggg ggaaatccgg
     7081 taatcaacaa tgcattaagc acgccgcttg ccagtacgcc gatcaccgca aaatgcccgc
     7141 accaggaaaa acgcatcagc gcctgaatgg cctggtgtcg ccaacggcct ttgatgagtt
     7201 gcatacacca gagcaccggc agtaatcccc caaaccacgc cgccgcacaa atcaggtgaa
     7261 tcgcgtgatt agtctgatgg attttcgccg ttaccccttc attcagtgtc gcatgcccga
     7321 ttcccgccag cagaataaat tgcgcggtag tgagcataaa cagcaaacgt ggcatattcc
     7381 gcggttgcat aagggcgacg atcaacgtta cgagggcgag aacaatttgc cataaccaga
     7441 cgccaccaaa ctgcgtttgc aaaacagcct gccaaatgtt tggcgaaaat acatctgccc
     7501 atcccgttcc catcaacccg ccctgaacgg cgagcattgc agtggcactg atcaaactcc
     7561 agacggcagc gtgctgttgt aaacgcagaa aacgcttcgc cagcaagcga cgaatcgtta
     7621 agggggccag ccaggcaccg tacattgcga agccaaagac cagcatcagc gaggtgaaat
     7681 ggataaatcg cagcgcgatc caggtaaacg ccagcatgat ttatttcacg ctaaaggtgt
     7741 agtgtccttt cgttttatgc ccgtccaccg aaacaacatg ccagtcaacg atgtaggttc
     7801 cgggtttcag cgaatcggcc agtggaacaa tcagttgctt ttggtcctgc tcatttcgct
     7861 tcgccggtaa tgttttaata ttttcgtttt ttggccccgt gatttttgca ccactgaatc
     7921 ctgtttcaac accttccgag aagtttaaag tgattgcctg cggtgcagct gtcacttgcg
     7981 cgtttgccgc aggatactga tgcgttaaat gcgcatgtgc ccagacggaa ggtgtcacca
     8041 acgaagtggt caggattgcc agcgcgtagc gaagggagcg tgcagttgaa gtcatattat
     8101 ctattccttt ttgtaataac ttttttacag agcataacct tgtctaatgt ctgagtcgag
     8161 gatcatcaat tccggcttgc catcctggct cactcttagt aacttttgcc cgcgaatgat
     8221 gaggagatta agaatgctga agaatctggc taaactggat caaacagaaa tggataaagt
     8281 gaatgtcgat ttggcggcgg ccggggtggc atttaaagaa cgctacaata tgccggtgat
     8341 cgctgaagcg gttgaacgtg aacagcctga acatttgcgc agctggtttc gcgagcggct
     8401 tattgcccac cgtttggctt cggtcaatct gtcacgttta ccttacgagc ccaaacttaa
     8461 ataaaactta tacagagtta cactttctta cataacgcct gctaaattat gagtattttc
     8521 taaaccgcac ccataatttg cagtcatttt gaaaaggaag tcattatgtc gttctggaaa
     8581 gttgcagcag cgcaatatga gcctcgtaaa acctcactca ccgaacaggt agctcatcat
     8641 ctggagtttg tgagggccgc cgccaggcaa caatgtcagc tgttagtttt tccatcgctt
     8701 tctttactgg ggtgtgatta ttcgcgacgt gcccttcctg ccccacccga tctctcactg
     8761 ttagacccgc tttgttatgc cgcaacgacc tggcgaatga ccatcattgc cggccttccc
     8821 gttgaatata acgatcgctt tattcgtgga attgcggtgt tcgcaccgtg gcgaaaaacg
     8881 cctgggatct accatcagag tcatggtgca tgtctgggtc gtcgttccag aaccatcacg
     8941 gtggtcgatg aacaaccgca aggtatggat atggacccga cctgttcact gtttactaca
     9001 gggcaatgtc tgggagaacc cgacctgctg gcgtcggccc gtcgcttaca atttttttca
     9061 catcagtact cgattgccgt gctgatggca aatgcccgtg gtaacagtgc actatgggat
     9121 gaatatggtc gtctcatcgt tcgcgccgat cgtggttcat tattgttagt cggtcagcgt
     9181 tcatcacagg gttggcaagg cgatatcatt ccattacgct aggctttttc ggcctggagc
     9241 atgccatgtt gcgcattatc gatacagaaa cctgcggttt gcagggaggg atcgttgaga
     9301 ttgcctctgt tgatgtcatt gacggaaaaa tcgtcaaccc catgagccac ctggtgcgcc
     9361 ccgatcgtcc tattagtcca caagcgatgg cgattcatcg catcaccgaa gccatggtcg
     9421 ccgataaacc gtggattgaa gatgtgatcc cacactatta cggtagtgaa tggtatgtcg
     9481 cgcataacgc cagctttgac cgccgcgtac tgcctgagat gcccggtgag tggatttgca
     9541 ctatgaaact ggcccgtcgt ttgtggcctg ggatcaagta cagcaatatg gcgttatata
     9601 aaacacgcaa gctcaatgta cagacgccgc cgggcctgca tcatcaccgc gcgttgtatg
     9661 actgttatat caccgccgcg ttgcttatcg atattatgaa cacctccggc tggacggcag
     9721 aacagatggc cgatatcacc ggacgtccgt cgttgatgac gaccttcacc tttggcaaat
     9781 accgtggcaa agcggtttcc gacgttgccg aacgcgatcc gggctatctg cgctggttat
     9841 ttaataacct ggacagcatg agcccggagc tgcgtttaac actgaaacat tatctggaaa
     9901 atacttagtc cgcaggcgta gcgggtaatg ttccctgcgc cagcgcgacc agaaaagcat
     9961 attccatcgc tacgccttcg tacgatttaa agcgaccaga tttaccgcca tggcctgagt
    10021 ccatgtcgat acagagcaat aaaagatggt catcggtttt cagctcgcgc aatttagcga
    10081 cccattttgc cggttcccaa tattgcacct gagaatcgtg caaaccggtc gttaccagta
    10141 aatgcggata agcctgtgcg gtgacgttgt catacgggct gtagcttttc atgtactcgt
    10201 aatattgcgg atcctgcgga ttaccccact cttcaaactc accagtggta agaggaattg
    10261 attcatcaag catcgttgtt acaacatcaa caaacggtac ctgggcgata acgccgtgga
    10321 ataattccgg gcgttgatta attgcaacgc ccatcaacat gccccccgca ctcccgccca
    10381 tcgcataaca aagcgaagga gagccatagc ccagttttaa caatgcatcg caggcatcaa
    10441 gataatcatt aaacgtattt ttcttcttca gaaattttcc gtcttcgtac cattgttgcc
    10501 ccagctcacc accgccgcga acatggacaa tggcgtagac aaagccacga tctaacaaac
    10561 tcaagcggct aaaactgaaa tcggcatcaa tacttgcgcc gtaagaacca tagccataca
    10621 ccagcaacgg gttgtgtcct ttgcgaaaat gtttgcgatg gtagaccaac gaaaccggaa
    10681 cttcgacgcc atcacgggcg actatccaca ggtgttcact gcggtaattc gccgcatcaa
    10741 aaccgggcac ttctgtttgt tttaatacac gacgctcacc ggtatccata tccagttcaa
    10801 acaaagtgtc tggtgtggtc atggaagaat aaccataacg caatcgcgcg gtttcaggtt
    10861 ctggattgta ggcaatccag gtcacatagg ctggatcatc aaaggcaata ccaatgactt
    10921 cccgggtctt gcggttaatt tggcgcaaac tggttaaccc gcgctgacgc tcttcaacca
    10981 ccagccagtc ggtaaacagc gtaaaccctt ccagcatgat gttttcgcgt ggcggaatta
    11041 actcttccca ctgttgctca tcacgcatac gggtacggta taagccaaag tttttgccgt
    11101 ggcggttgga acgtagataa aaacgatgct ggtagtgatc aaggctgtat tcgtgatctt
    11161 tgcggcgcgg cagaaaaaca aacggctcgg catcggccat ttccgcgtcc agcaggcgaa
    11221 cttcactggt ggtggcgctg gccaaatgaa tgactacata gtgcttcgag gtcgttttat
    11281 gcaggctgac gtaataggta tcgtcttttt cttcgtagat cagtttatct tgcgatgctg
    11341 gcgtaccgat ggcgtgacgc cagacctgat aaggcagcag cgtcaccgga tgcttgcgaa
    11401 catagtagaa agtccaggag tcatttgccc agacaaagct gggttcaacg ttatccagca
    11461 gttccgggta ccagttacca gtttccagat tacgaaaacg aatgccgtac tggcgtcggg
    11521 aaagaaaatc ttctgccagc gccataatgg tgttatcggg cgtaatcgcc attccgccca
    11581 tcgaataaaa ctcactatga gctgcgcgct tattggcatc gagcaatgtt tcccactcat
    11641 cccactcttc actgaatgcc gattgacgct ggtagatagc atattcacag cctggttcat
    11701 aaatatgccg atagcggtag ccatttttga tgtagggcgc agaaacttct cgttgcggaa
    11761 tgcggtcgat gatttccttt aagatgcgat cctgcaaggc ttgttgtgag gccatcaccc
    11821 gatgaccgta actattttct tgttgcaggt agtccaggac ttctggctga gaacgcgtat
    11881 cgtcccgcag ccagtagtaa ttatcgatgc gcgtatcgcc atgaagcgtc atggcgtggg
    11941 gaatgcgggc ggcttttggt agcatgttat tgttctttct ggttgaaaca tcttataagg
    12001 gtggcaaaac tcaccgggga tgcaagcgaa acaggggagt cattgcttag atgatgacag
    12061 gtaatggcgc ggatatcgaa tgttatgcag acagagaaaa tcagcctgtt caaatggctg
    12121 tgcgattctg gatagcccga aatagtcaac ttcaggctat ccagagagcg gaattattcc
    12181 gccaaagtgc gtttttgctg ttcgagatcg cgttcaatgc cgtcacgaac atcctggggg
    12241 attttcagcg cgtcacccag tgcattcagg taactgcgtt ccataaaatg gtcaatatca
    12301 atagccgcgc aactcaggaa atagatttcc agcgcctctt cttcattgcg gaccccggta
    12361 gccaggcgtt gtggatccag cggttgttcg attgcctgct caatgagtac acgcccctgc
    12421 tcttccacgc cggcttcacg caattgctgg tcgattgccg cacgttcttt ggcatcaata
    12481 tgaccatcac ttttagcggc aaagaccagc gcaaggatca aacgttctgt acgctcatcc
    12541 agcggcgtac tttgcgcgcc aaactgcggt tcgtcctgat gcgccgcgcg aattttatct
    12601 ttgtatttat tccacagcac cgtacccgct accgctccgc cgccaaccag taacgcgttg
    12661 gtgccatatt tcgtaagaag tttacgtgct gatttatttg cgaccagcag cccagccagt
    12721 ccgcctaatg cgcctggcac taacagtttg cccaatccct gatccgcaga cgaggaggta
    12781 gaagaactgc tttgcccaag cagggattgc agttgattta accagttagc catatttgct
    12841 cctcaataac catttttagt gcctgtagca tagcggatga ggatgtcaga aaatgtatat
    12901 cgagacaaaa gatgcgcaaa taagcggggc ggtaggccgt tattcaaaga aattcgcgcc
    12961 aggtaaggta tttactgtat atcaggtgat aagggtattt taggtgaaag tagtttgtcg
    13021 ttttgagtgg cattgtttga tgctgattgc ccgatgcgac gctgttgcgt cttctcaggc
    13081 ctacgattgt ggtgcgttct ggggcggaca aggcactcgc gccgcatccg ccaacggtca
    13141 atcgaattat cagaagccat taacgccgct gatattccgc cattcccgct ttgcagtcca
    13201 cgctgacctg gtaatgaata tcggcacttt taccacgcac ggttagcggt actgaccatt
    13261 tatcatcttt accctgaatg tcctgcaaac tgacccaagc cacgggatcg gcctgaccga
    13321 caattttttg atcatctgcc caacgcgcca cgcgattttg ttgataatca cgttttacgc
    13381 tcgcggcaat tccggcagca tccagacctt cacactttgg gaaagtgacc gacttgctgg
    13441 tttcattatt ggcagcgaaa actgatgcgc aggcagaaac caacaacagc cctaaaaacg
    13501 cccctctttt tttcatgttt ttctccatag cacaatgatt caggagaaag catggtacaa
    13561 attgtcagga gcgcaagttg cttcaggccg cgtgtgaggc atcttcctct tcggattcag
    13621 caccgacgac ctgtggggac ggtaatttgc cggttttcag aatggtgctc agcacatctt
    13681 tttgttctgc cagccatagc gaaagggctt cacgttgctc gtcttccatt tgcactggag
    13741 aatttgtcag ccaggtgtca agcagatccg ccgaatcaag cattttatca taagcatcgg
    13801 cttccttttt gctggtaaac gacattttct cttcgccctc acgaatgact acgtatttaa
    13861 cttcaaccgc
//
LOCUS       DAZGLJ010000122        13593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000122 DAZGLJ010000000
VERSION     DAZGLJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.122"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..261
                     /locus_tag="ACHK49_003104"
     CDS             <1..261
                     /locus_tag="ACHK49_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263714.1"
                     /translation="IEGHDIPSMAWEFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMA
                     YIDIRLGVIICGLSGWNITSRVLRYLIVDRTAAPAGISAEVA"
     gene            261..776
                     /locus_tag="ACHK49_003105"
     CDS             261..776
                     /locus_tag="ACHK49_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263715.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFCFKGLRFMDDKYPFHRDLWRKPTASNGWSCTPRTSRIPKALRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAIMFGPVKWFCVDDVIYLECGVKPANQKMTEILSD
                     EFYAAEKRVRG"
     gene            780..1331
                     /locus_tag="ACHK49_003106"
     CDS             780..1331
                     /locus_tag="ACHK49_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFO9263716.1"
                     /translation="MMILQPMGRKGQAPAHVRAWTPEEDALLIALYPSTPVKDIAVRV
                     KRSFWGVHNRIVLLRGTYPELLKCKRPRFKHDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVRLIRELRDAESPRRLTFREIGEKFELSE
                     GTVSFIYHRRRTAEDVVLRELMP"
     gene            1328..1639
                     /locus_tag="ACHK49_003107"
     CDS             1328..1639
                     /locus_tag="ACHK49_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263717.1"
                     /translation="MITTLFVESDEPLVCAVGMPVCGGTLTGVYFGDLRGYPWHSLND
                     AFPPDMEAVVLIVQYGDQQELRIGHMGYEGFFIDEETGACLEDEDGQVTHWCHISALP
                     E"
     gene            1654..2004
                     /locus_tag="ACHK49_003108"
     CDS             1654..2004
                     /locus_tag="ACHK49_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263718.1"
                     /translation="MRSYIDNEKLKTISDCLSLLAKIKETIEEIKFQLEYEPCGDDTL
                     RNSARKALAVFQKQRRTVEYRLAVLRQEEKERNIRRHELVNDFLVRELKERVPESVFF
                     ECEAVARSKALETD"
     gene            2020..2352
                     /locus_tag="ACHK49_003109"
     CDS             2020..2352
                     /locus_tag="ACHK49_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263719.1"
                     /translation="MSIELRSSYEYRKILIAGGMKPEDAEKIVSFMDKECDKRDMPEI
                     IMDDMILHSAVALSPLWIVHSLAEIAKGTDKQAAVAALQTLNEMRISPRPTLIHMILS
                     SMEDKANE"
     gene            2345..2542
                     /locus_tag="ACHK49_003110"
     CDS             2345..2542
                     /locus_tag="ACHK49_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263720.1"
                     /translation="MNSLPAGWARPLMARKHHFFKTGENISICGRWLYLAHNREPDTF
                     ESPDDCAECRRRVNKEKDNGQ"
     gene            2532..2828
                     /locus_tag="ACHK49_003111"
     CDS             2532..2828
                     /locus_tag="ACHK49_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263721.1"
                     /translation="MDNSSLLFWCLYITSFFGAFVITRWLCRKIICFFDKRHPVERAA
                     DALIQQAIVLYNGEFFCRITSRDGWHIMIIPPTHHARWDEAEKAFHVRKKVNTV"
     gene            2825..3334
                     /locus_tag="ACHK49_003112"
     CDS             2825..3334
                     /locus_tag="ACHK49_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFO9263722.1"
                     /translation="MRGKLISAIHVAKRELALDDETYTSALLAATGKTSCRDMSPDEL
                     SRVLDVFKKRGFKVRQNPVNRALKPGTVTAKIRAIWKVMHRQGFITDGAETALNRWVK
                     SQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMGMPERTLMGYDAVC
                     RQYEKSLPR"
     gene            3404..3829
                     /locus_tag="ACHK49_003113"
     CDS             3404..3829
                     /locus_tag="ACHK49_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO9263723.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDVLKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAELRNNEIFSRWD
                     RGEKIESLRRHYRMSETQIYTVIREQRRLHLVRTQPPLF"
     gene            3901..4401
                     /locus_tag="ACHK49_003114"
     CDS             3901..4401
                     /locus_tag="ACHK49_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9263724.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLELLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            4436..4864
                     /locus_tag="ACHK49_003115"
     CDS             4436..4864
                     /locus_tag="ACHK49_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263725.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHSEGQQSV
                     VRRSLEAVPAASAPVPDDVADRVRRAVCEIRACEAGADPR"
     gene            4848..5066
                     /locus_tag="ACHK49_003116"
     CDS             4848..5066
                     /locus_tag="ACHK49_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263726.1"
                     /translation="MPIPAEWLADCLVPPAPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            5077..5304
                     /locus_tag="ACHK49_003117"
     CDS             5077..5304
                     /locus_tag="ACHK49_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO9263727.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKRRAMDAPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            5285..5593
                     /locus_tag="ACHK49_003118"
     CDS             5285..5593
                     /locus_tag="ACHK49_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFO9263728.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTQ
                     "
     gene            5590..5880
                     /locus_tag="ACHK49_003119"
     CDS             5590..5880
                     /locus_tag="ACHK49_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFO9263729.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRATVPGVKKPRARG"
     gene            5883..6464
                     /locus_tag="ACHK49_003120"
     CDS             5883..6464
                     /locus_tag="ACHK49_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFO9263730.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKA"
     gene            6464..8128
                     /locus_tag="ACHK49_003121"
     CDS             6464..8128
                     /locus_tag="ACHK49_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263731.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLLEALNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            8128..9717
                     /locus_tag="ACHK49_003122"
     CDS             8128..9717
                     /locus_tag="ACHK49_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFO9263732.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAVLDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIP
                     DPWVREQTGIPQPAPGEAIFRVRQSGNEPAQTDKEMPPEKQEKTEQTALAARLPEAKS
                     SPRDELDDMGDAVPARRLQEAIDPVLEPVIEAIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGTGD"
     gene            9701..11032
                     /locus_tag="ACHK49_003123"
     CDS             9701..11032
                     /locus_tag="ACHK49_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFO9263733.1"
                     /translation="MAQGIDLGYAATLPSKEAVAYFRAKGAHISWNWFETDADVHARS
                     FTAAKVARLDVLTTLQAEVQRAVDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGQVFRYDDPFWNTHYPPNGWNCRCRVRPLSQTRLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSMKHQNDGIALTPEDFGRLPAMLAKPKAVLWDKRHNNLMYIVESKDS
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGDID"
     gene            11154..11627
                     /locus_tag="ACHK49_003124"
     CDS             11154..11627
                     /locus_tag="ACHK49_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO9263734.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVAASLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            11804..12928
                     /locus_tag="ACHK49_003125"
     CDS             11804..12928
                     /locus_tag="ACHK49_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFO9263735.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINRATVERMVSRVVALNQPVKIDYNHQTLIKGHQAPAAGFVMASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDENTGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDNLKTQVASLSAELETAKGTPAGGIIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQVLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAALTSLQTD
                     TVTVPDKKTATAALSAEDIAAAKLLGKTEAEFLKMKEEMQ"
     gene            12928..>13593
                     /locus_tag="ACHK49_003126"
     CDS             12928..>13593
                     /locus_tag="ACHK49_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO9263736.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAVQPDELVFAALRDGISAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGA"
BASE COUNT         3178 a         3473 c         3965 g         2977 t
ORIGIN      
        1 attgaggggc atgatattcc gtcaatggcg tgggaatttg ctcagggcgt tctgacctgt
       61 gaaaccgttt atgtggtggc tgaagttgat gagcaggcca tgaaagaagg cgtgccccag
      121 tttgtgatgg cgtatatcga catacgcctg ggtgtcatta tctgcggttt aagcggctgg
      181 aatatcacct cgcgcgttct gcgttacctg attgttgacc ggacggccgc gcctgccggg
      241 atatctgcgg aggtggcgta atgttcttta aaacatcaaa cccttccgcg ctggccgcgt
      301 ggcaaaaata tcagcaggac tgccagaaag ttaaggatga ggcaaaacgc cttgaggccg
      361 tgctgaatgt tgcgtgccgg tcggtatttg tatccggtat cagtggcttt tgttttaaag
      421 ggctgcgctt tatggatgac aaatatcctt ttcatcgcga cttatggcga aaaccgactg
      481 cgtcgaatgg ctggagctgc acaccgcgca catcacgtat tcccaaagcc ctgcgcgttg
      541 cctctgacga acttaacagt ctgtggcgtg aatattcgcc cgtcacgtat gccagaaccg
      601 atgcactgtt gttctggctg ggtattgatt tctcggcaat catgtttggc cctgtgaagt
      661 ggttctgcgt tgacgatgtg atttaccttg agtgcggcgt aaaacccgca aatcagaaaa
      721 tgaccgaaat tctgtctgat gagttttatg ctgctgaaaa gcgagtcagg gggtgatgta
      781 tgatgatttt acaacccatg gggcgaaaag gtcaggcacc tgctcatgtc cgtgcgtgga
      841 cacctgaaga agatgcgctg ctgattgcgc tttatccatc caccccggtt aaggatattg
      901 ctgtcagggt aaaaagaagt ttctggggtg tacataaccg gattgtttta ttacgcggta
      961 cttacccgga gttgctcaaa tgcaaacgtc caagatttaa acatgatgaa gataaattta
     1021 tccgaaaaaa tgccagaacg atgaccggta agcaaatcgg agaatatctg gggcgagacc
     1081 gggattctgt ccacaatcgg gcgcgatata ttggcgtaag catgaaaaaa tacggagagt
     1141 tgctgccctt cacccgcata cctgacgatg atgttcgtct tattcgtgaa ttacgggatg
     1201 ctgaatcacc acggcgtctt acctttaggg aaatcggcga gaaatttgaa ttatccgagg
     1261 gcacggtgag ttttatttat caccgtcgtc ggactgccga agatgttgta ttacgggaat
     1321 taatgccatg ataacgacat tatttgttga atcagatgaa ccccttgtgt gtgccgtcgg
     1381 aatgccggtc tgtggcggaa cgctgaccgg tgtttatttc ggggatttgc gcggttatcc
     1441 ctggcattca ctgaatgatg ctttcccgcc tgatatggag gctgtcgtgc tgattgttca
     1501 gtatggtgac caacaggaac tgcgcatcgg ccatatgggg tatgaaggct tttttattga
     1561 tgaagaaacc ggagcctgcc ttgaagatga ggacggacag gtgacgcact ggtgccatat
     1621 ttcagcttta ccggaatgac gggaggtgaa tgcgtgagaa gttatattga taatgaaaaa
     1681 ctgaagacaa tcagcgactg tcttagcttg ctggcaaaaa taaaagaaac cattgaagag
     1741 attaaatttc agcttgaata cgaaccctgt ggcgatgata cattgcgtaa ctctgccaga
     1801 aaagcactgg ctgtctttca gaaacaacgc cggactgttg aataccgcct tgctgtattg
     1861 cgccaggagg aaaaggaaag aaatataaga cgtcatgaac tcgttaatga ttttcttgtt
     1921 cgtgaattaa aggagcgtgt gccggagtcg gtattttttg agtgtgaggc tgttgcccgt
     1981 agcaaagcac tggaaactga ttaaacaggc aggtgaatga tgagtattga attaagatcg
     2041 tcttatgaat atcgtaaaat tctcatcgcc ggaggcatga aaccggaaga tgcagaaaaa
     2101 atcgtttctt ttatggataa agaatgtgac aaacgggata tgccagaaat tattatggat
     2161 gacatgattc tgcattcagc cgtagcgtta agcccattat ggattgtgca ttctcttgct
     2221 gaaattgcca aaggcaccga taaacaggca gctgttgctg cccttcagac gcttaatgaa
     2281 atgcgtatat ctccacgccc gacattaata catatgattc tgtccagcat ggaggataaa
     2341 gcaaatgaat agtctacccg ctggatgggc gcggccacta atggccagga agcatcattt
     2401 ttttaaaaca ggcgaaaata tcagtatatg tggacggtgg ttatatctgg ctcataatcg
     2461 cgagccggat acatttgaaa gccctgatga ctgtgccgaa tgccgcagaa gagtgaataa
     2521 ggagaaagat aatggacaat agttctctgt tgttctggtg cttatatatc acatcctttt
     2581 tcggcgcatt tgttattacc cggtggttat gtcgaaaaat catctgcttt tttgataaaa
     2641 gacatccggt tgaacgggcg gctgatgcgc tcattcagca ggccattgtg ttatataacg
     2701 gtgaattttt ctgccgaata acaagcagag atggctggca cataatgatt attccgccaa
     2761 cacaccatgc ccggtgggat gaggcagaaa aggctttcca tgtccgtaaa aaggtaaata
     2821 cggtatgaga ggaaaactga tatccgccat tcatgtggca aagcgcgagc ttgccctgga
     2881 tgatgagact tatacatccg cgctgctggc agctaccggc aaaaccagct gccgggatat
     2941 gtcaccggat gagttatccc gcgtgctgga tgttttcaaa aaacgcggtt ttaaagtgcg
     3001 tcagaacccg gttaaccggg ccttaaaacc gggtacggtg accgccaaaa ttcgcgccat
     3061 ctggaaggtg atgcaccggc agggctttat caccgatggt gcggaaaccg cccttaaccg
     3121 ctgggtgaaa tcgcagacgg ccgcgcagaa cggcggcgaa ggtgtggcaa actggcagtg
     3181 gctggagcaa caccccgccc tggcctcaga tgtgctggag cgtctcaagc gatggcaccg
     3241 ccgcaaaatg ctggccgcga tgggaatgcc cgaacgcacg ctgatggggt atgacgccgt
     3301 ttgcaggcag tatgaaaaat cacttccccg ttaactccaa atcccgccac aacgcgggat
     3361 ttttatttta aacttaccgg gaacgcgaga accggaggct gatatggcag aaactcagat
     3421 gagcatgttt ggtggtgaca gtgagcaact gcacgccctt atcgaccgcc ttgatgacat
     3481 ccctgatgat gttctcaaaa agaactggcc gcggaccctg tcagaactgg ttgaagtcac
     3541 cggcgcagaa cttcagcgtc aggggattga gccggtactg gccggtaaac tggcacgcaa
     3601 ggtggctgcg gctcaggccg cctatatggg cggacggggt tattacctgc cggtcgggga
     3661 atctctcttt gccgagctgc gaaacaatga gatattttcg cgctgggacc ggggcgagaa
     3721 aattgaatcc cttcgccgcc attaccggat gtcagaaacc cagatttata ccgtcatacg
     3781 cgaacagcgc cgtctgcatc tggtaagaac gcaaccgcca cttttctgat attccgcaaa
     3841 gcctgccgcc tctccttcac cgttacgctg actcagagaa catcatgaga cagcgtaaca
     3901 atgccaaaac tccccgcacc actgcgtaaa aagctgattg cccttgttct ggccggtgcc
     3961 gggacgttca cgattgccac gcattacacc ggttactggg aagggaaaga aaactccacg
     4021 tatatcgatc ccaccggtac acccaccatc tgttacggcc ataccggccc ggatgtgaaa
     4081 ccgggtatga ccctgacaga tgaagaatgc ctggaactac tggaaaagga catgaaatgg
     4141 gcctttgcgg ccattgatcg gcgtgttcag gtgccgctta cccgtggtca gacggtggcg
     4201 ctggcttcgt ggatcttctg ggccggtgaa acgaactttc gcaactccac gcttctgcgc
     4261 ctgatcaatg ccgggcagat gcccgcgtcc tgtaagcagt atatccgctg gatttattca
     4321 aagggggtga aactccccgg ccttgaggcc cgccgttcgg cggatgaatg gttatgtcgc
     4381 tacgacttgc cgaaagtctg aaccgcttct ggcgaccgct catgatagcg ctgctgtgtg
     4441 cggtactgct gttacggggt gtcctgtggc tgcggtgaat aatgccttac cggccctgat
     4501 tgccggggcg tgcatggcgg cgctggggat tatcgctgta ctggttgccg ctatatgggg
     4561 aatgcaccag aagacgcagc gccttgaaga caataatcag gtgcttgtgc gtgagcggga
     4621 tgaggcgcgt caggtgctgg caaatcagca gcacaccctg caactcattt cacaaatcag
     4681 tgaggcggcc acgaatgaaa aacaacagaa cattcagcac agcgaggggc agcagagcgt
     4741 tgtccgccgg tcgctggagg cagtgcctgc ggcctctgcc cctgttcctg atgatgtggc
     4801 tgatcgggtg cgccgggccg tctgtgaaat acgtgcctgt gaagccggtg ccgatccccg
     4861 ctgaatggct ggctgactgc ctggtccctc ctgcgccgga gccgttcacg tttggggcat
     4921 cggtcactta caacctgcaa ctgctggcgg tgatcaagaa ctgcaacgtg gacaaggcca
     4981 gtattcgtcg tctggaggcg cggcgacaac atgaatttac tgatatggcc ggaacgcctg
     5041 ctgttccggc aggaaagacg aagtaaggaa aaaggtatgg atgattcaga tcgtgctcag
     5101 gctgtgatgg agcggggatc agaacgcgcc ctttgtaacc ggctgacacg taagcgccgg
     5161 gcaatggatg ctccgggcag acgtgtctgc gcggactgtg gcggagagat ccccgccgca
     5221 cgtcttgttg ctgtgccgga tgccatccgt tgtgtgaact gccagaacat catggaggcc
     5281 cgtcatgtgg gtcagcatcg ttaaagatta tgttgtgccg atcctttcgg cgacggcaac
     5341 cgctggcggg atattcatgg cgctgatgcg caaaacgttt gtcccccgcg aggcctttga
     5401 aaaactctcc gaccgcgttg agaaggtgga aacccgcctg tcgtcactgc cgacagaagc
     5461 cgaggtcaac cgtctgaatg tggaaatcgt gaccctgcgg ggtgaactga aaaccacgaa
     5521 cgccacgctc cgctctgtct cctatcagaa cgaactgctg ctggagcagg cggtaaggaa
     5581 aaaaacacaa tgagtgattt cattactgaa gatcagcgtc ttgtcattct gcggtcgctg
     5641 gcggattaca acggcgaact gggtgaatcc gtgctccagg actgtctgga tgattacggt
     5701 caccgcgtgt cccgcgacac cgttcatact catattgcct ggcttgccga gcaggggctg
     5761 gtgcgtaagc gcacgctcgt taatggttat ttcatcgctg aactgaccgg ccggggtcag
     5821 gatgtggcgg aaggccgcgc caccgttccg ggtgtgaaga aaccacgcgc aagggggtag
     5881 cgatggacaa gccgacgcgt ggccgcgtgc gcaaggtgga tttgctcccc gacagcatcc
     5941 gtaagccact gctggaaatg ctgcgtgaaa aacgtctgac gcaggtccag atccgcgagg
     6001 aaatcaaccg tctgatccgc gaggcaggcc tgccggaaga gcagcagctt tcaccggcgg
     6061 ccatcagccg ggaagcctcc cgtaatgagc tgattgcccg taacctgcgt gatttacgcg
     6121 agcagacaaa agccatgatg gccgaactgg gggacaagcc gaccggtgaa accacggcac
     6181 tgattctgga gatgtcccgc gccctgatgt accgccgtct gcgtgccgcg accgaatccc
     6241 tgaacagtga cagcgacgtg gatatgcgtc ttattaagga catcctcctg tcagcacagc
     6301 gtgcggaaag tgccgccgag cgcagcatta aacgtgaaaa agaaatccgg gctgcatttg
     6361 ctgaagagat ggcaaacgcg gttaccgacg agctgcgcgg cgtggacggg atgagtgaac
     6421 agcttgagtc ccgtattaag ggaattctgt tgggtaaagc ctgatgaata acgccgaaga
     6481 actctccccc ttactgacga ataccgtctc cacccgcaaa attgatctgg ccggtgaaaa
     6541 agccctgctg ggcgtggatg tgccggacag ccttgatttg cccggtgata tgccggtgtt
     6601 tctggattat caggcccgct ggtttgagga tgaaagtgag gtctgcatcg cggaaaaatc
     6661 ccgccgcacc gggcttacct gggcggaagc cgggcgtaac gtcattaccg ccgcaaagcc
     6721 taagcgtcgt ggtggccgta atgtgtttta tgtggggtca aagcaggaga tggcgctgga
     6781 atatatttct gcctgcgcac tgttctcccg tgcctttaac cagctggccg atgctgatgt
     6841 gtatgagcag accttctggg atcgggataa aaaagaagaa attctgacct acatgatccg
     6901 ctttccgaac agtggattca aaattcaggc actgtcttcc cgtccgtcaa acctgcgcgg
     6961 ccttcagggg gatgtggtga ttgatgaagc cgcgttccac gagtcactgg atgagcttct
     7021 caaggcggca atggcgctca ccatgtgggg cgcgcgcgtg cgtattatct ccacgcataa
     7081 cggcgtcgat aatctgttta atcagtacat tcaggaagcc cgcgagggac gcaaggatta
     7141 cagtgtccac cgcataaccc tggatgatgc cattgcggac gggctgtacc gtcgtatctg
     7201 ttatgtcacc ggccgcgaat ggtcaccgga aagcgagcag aagtggcgtg atgatctcta
     7261 caaaaacgcc ccgacccgtg aggatgccga cgaggaatac ggctgtatcc cgaaaaaatc
     7321 cggcggtgcc tatattcctc acgcgcttat tgaaatggcg atgatccgcg acatcccgat
     7381 tctgacgttt gaagccccgg acgacttcat cagccgtgcc gcatggctgc gtgaatcgga
     7441 ggttttaacc tggtgtgaag aacatttaaa accgctttta gaggcgttaa atccccgttc
     7501 ccgcttcagc ttcggggaag actttgcccg caccggtgac ctctcctgct ttgtgctgct
     7561 ggaaatcact gaatccctgg caaaacgcga ggtgtttcgc gtggagctgc gcaacctgcc
     7621 gtatgcccag caggagcagg tgatgatgta catcctgacc cgcgttccgg cgctggtcgg
     7681 tgcggcgttc gacgccaccg gtaacggcgg ctatctggcc gaagccgcgc tgctggcctt
     7741 tggcccggac atcatcgact gcgtgatgtt atcgccgaag tggtacggcg agtggatgcc
     7801 aaaactgaag gccgagtttg aggatcagaa tatcctcgtc gcccgccatc agaccacgct
     7861 tgatgatttg cgccatgtga aggtggtgaa cggtatcccg caaatcgaca aggggcgtac
     7921 gaaggaccag aacgcaacag ctgtgaacgc ccgccgccat ggtgattttg ccgtggcgct
     7981 ctgtatggca aaccgggcgt catacatgga gggctttatc ctggatgaat cagcctgcca
     8041 ggcgctgccg gagcggtcgc gggccatgga gggcggttat cgtgatgacg atgaggcata
     8101 tcatgaattt gatcgggggt gctggtagtg ggacgcataa ttgatcttga cggaaaacct
     8161 ttctcctttg acccggagat gcagagtgcc gtgctggata ttccgcagat tgccagccgt
     8221 tatattgaac atccggcctc gggtatcacc ccgaaccggg cggcacagtg ccttcgcggg
     8281 gctgaacgtg gcgatctgat tgcccagtcc gatctggccg ctgacattga agaaaaggat
     8341 acccatcttt ttgcagagct gggcaagcga cgtcttgcca ttcagggggt gccctggagt
     8401 attgagccgc ccccgaacgc cagcgcgaat gagaaaaagg acgcggaaat gctcgacgaa
     8461 tacctgcatt ccgccgactg gtttgatgcc atgctgtttg atgccaccga tgccatcctg
     8521 aaaggctatt cctgcatgga gattgagcac gggatgctcg gtaaaatgca catcatccgc
     8581 gccatccgct ggcgtgacag cgggcatttc tgccttaacc cggatgattt gagcgaactg
     8641 cggctgcgtg acggcagcca tgccggggtg gcgtttcagc cctttggctg gatagtgcat
     8701 cagtcgcgtt cacgcactgg ctacggtggt gcgacggggc ttgtcagaac gcttatctgg
     8761 ccgttcattt tcaaaaacta ttccgtgcgc gatctggctg aatttctgga ggtgtacggc
     8821 ctgccgatga aggtcggtaa atacccgtcc ggggcaacac cggagcagaa aagcgccctg
     8881 atgcgggcgg tgatggatat cgggcgacgt acaggcggga tcatcccggc cgggatgtcg
     8941 ctggagtttc aggcggcagc gaacggtcag gccgatccgt ttgaaaccat gatttcgtgg
     9001 ggggagcgtt ccatctccaa agctatcctc ggcggcacgc tgaccacgga agccggagac
     9061 aagggcgcac gttcgctggg tgaagtgcat aacgaggtgc gccgggaaat cagggattct
     9121 gatttacgtc agctggccgc cacgctgaac cgcgatctgg tgtatccgct gtatgccctg
     9181 aacaccgccc acgccattga tatccgtcgc ctgccgcgta tctgtttcca gacaaaagag
     9241 ccgggggata tcaccaaaat caccagtgcg gtgatgcagc tcagtacggg gatggatatt
     9301 cctgatccct gggtgcggga gcagaccggt attccacagc ctgcccccgg tgaagccatc
     9361 ttccgtgtcc gtcagagtgg caatgaacct gctcagacag acaaagagat gccaccggaa
     9421 aaacaggaga agacagagca gacagcgctg gctgccagac tgccggaagc gaagagcagc
     9481 ccccgagatg aactggacga catgggggat gcggtgcctg cccgccggtt acaggaggcc
     9541 atcgacccgg tactggagcc ggtcattgaa gccatcagaa cgcgggggct ggcggaggca
     9601 ctggcagacc tgcctgccct ttaccgtgaa atggatgatt cccgcctgat gacgctgctc
     9661 agtgatgcca tgtttgctgc ggaaatgaag gggatgctgg atggcacagg ggattgattt
     9721 aggttatgcc gccacgcttc cctcaaaaga ggcggtggca tacttccgcg ccaaaggggc
     9781 gcatatcagc tggaactggt tcgaaacaga cgcggatgtt catgcccgtt cattcacggc
     9841 ggcaaaagtg gcacgcctgg acgtactgac cacactacag gcggaagtgc agcgtgctgt
     9901 tgatgagggg atttcacaga aagcatttat ccgcacactg acaccccgcc tgcaaaagct
     9961 gggatggtgg ggaaagcaga ttgtggtgga cagcgccggt aacgcagaag aagtgcaact
    10021 gggcagtccc cgccgtctgg cgctgattta caacgtgaac acccgcgtgg cttacaatgc
    10081 cgggcgttac acgcagatga tgaacaacac ggacacgcat ccgttctggc agtatgtggc
    10141 ggtgatggac agccgcaccc gcccggcgca ttccgccctt aacgggcagg tattccgcta
    10201 tgacgatccg ttctggaata cacactaccc acccaatggc tggaactgcc gctgccgtgt
    10261 ccggccattg tctcagaccc gtctggatgc aatggggtta tccgtttcat ccggtcagga
    10321 tcatctttcc acccgcaatg ttgaggctgg cgtggataag cagaccggag aagtcagaga
    10381 aatgccggtg accacatatt cagatggcac cagaaccatg acaccggatg tgggctggtc
    10441 atataacccc ggctcggcgg cgttcggcac agaccaggcg ttgatccgta aactgatcga
    10501 ggtgaaaagc ccggcgctgc gggaaatggt agtgcaggag atgaacaaca gcccggagcg
    10561 gcagctggcg ttccgcatct gggcaaaaaa catcatgaaa acccggcgag gcggtaacga
    10621 tatccgcacg ctgggcttta tgaccgaaag cattgcgcag gcggtggaaa gccggacggg
    10681 aacgccaccg gcccgcctgc tggcgatgag cggtaaaaat gtgctccatg cggacagcat
    10741 gaaacatcag aatgacggta tcgccctgac gccggaggat ttcggtcgat taccggcaat
    10801 gctggcaaag ccaaaagcgg tgctgtggga caaacgccac aacaatctga tgtatatcgt
    10861 ggaaagtaag gatagcagtg tgcagattgc cgttaatgcg ccgtacagcc tgaagcgtca
    10921 gccggataaa ctggatgtga ttgtgaatgc ttaccgggtt atcaatatgg ataagctgaa
    10981 atcggatatc cggggtggaa tgcttgaggt actggaagga gatattgatt aacggtcagt
    11041 ggcgggggtt gaacccgcat acatgatcca cccggaggca ggcaccgact ttaccagtta
    11101 agcgtacact gaccgcctgt ttattttata ttcatccttt atgcggaggc aatatgtcat
    11161 ccattgatgc cgctgtcgtt gttgacgtta cgcgtctcca gcgggtcttt gcccggcttc
    11221 agtttgtggg tggagggaaa gacctggccc gcagtgtggc ggcgagtctt ctgtcatcgt
    11281 cagaaatggc gtttgaacag gaaaaagagc cggacggcga acgctggcat gactggtcag
    11341 atccttaccg caagtggcgt acccgtaagg gatacatgcc cggcaaaatc ctgacgctga
    11401 acggcgatct ggcccgacgc ctgaccacgg attatggcga tacctgggcg ctgattggat
    11461 caaatgagcc ttatgcggcc attcatcagt ggggtggcct gcctggtatg ccaccgggac
    11521 cggcggccat tggcgcacgc ccgtatatgg gctttgatca ggtggcagag caggagatca
    11581 tggacgaaat cagaaaacgc tttaaaaagg ccacagaaac gccttaattg tttaaggtat
    11641 gcaaatgcat taccttaccc ccttcaggcg cgtgtcgtga ttttctaacc tgtatttaac
    11701 gggctttaaa atctgcgtga agcgcctttg tatttttccc ctggaagcac ttcctgaaat
    11761 cccgcaaaac ccgccctgaa aattcccggc ttatgctgcc ggaatgaaga cgaaaaacac
    11821 gcccaaactt gcttatgcca ttctgaatgc catcagcctg tccgcagacg gggacggtga
    11881 ctggtgtcag atcatgccag ccggtcgggt aaaggcccgt gacggtcgcc cggaaaaacc
    11941 ggccgagggc tggctgatta accgtgcgac cgttgagcgc atggtatccc gggttgtggc
    12001 gctcaatcag ccggtgaaaa ttgactacaa ccaccagacc ctgattaagg ggcatcaggc
    12061 accggctgcc ggttttgtga tggcctcacc ggagaatttt cgcttcagtg aagagcgtgg
    12121 cttcgaggtg cgcccgaagt ggaaccctcc ggcccttgaa catctgcgta ataacgaatt
    12181 cccctggttt tcaccggtga ttggctatga cgagaacacc ggcgaacctg tcgaacttcg
    12241 gatgctggct attaccggtg accccggtct gaccggcatg aatcctgtcg ccgcgctgtc
    12301 ggcggatgac ctttataacg ccttaaaccc tcctttaaag gatacctcca tgaatgagca
    12361 attacgccag ttgctgacgg cgctcggtct gaccgtggct gacggtgacg aatttacgcc
    12421 ggagctgggc acggcggcgc tgtctgccct caccgggatc aaaacccgtg cggatgcaca
    12481 cgacaacctg aaaacacagg tcgccagtct gtcggcagag ctggaaaccg caaaaggcac
    12541 accggctggc ggcattattg atctgacgaa atacgtgccc gttgaaacgt ataacgccct
    12601 gcgtaccgaa tacgttgcgc tgtcggcgca gcacggcagc accacgctgg agcaggtgct
    12661 ggacaaggct gaatccgaag ggcgcatctt caaaagcgaa cgcggctatc tggaacaact
    12721 gggcgggcag attggcgttg cggcactttc tgcacagctt gacgcccgcc agccggttgc
    12781 ggccctgacc tctcttcaga ccgacaccgt gaccgtgccg gataaaaaga ctgccaccgc
    12841 tgcgctgtcg gctgaagata tcgcggccgc caaactgctg ggtaaaaccg aagccgagtt
    12901 cctgaaaatg aaagaggaaa tgcaataatg cctaccccga ttacaccggc gatgatcaca
    12961 gccctgatga cgggctaccg ttctgatttt caggccggga tgtccatggc cccgtcgcag
    13021 tataaaaaaa ttgcgatgac cgtgccctcc acatcgaaat ccaacaccta cggctggctg
    13081 gggcagttcc cgcagttccg tgagtggatc ggctcccgcg tcatcgagaa gatgaaagcc
    13141 tatggctatg ccatcgtgaa caaaactttt gaaggcactg tcgccattag ccgcgatgac
    13201 ttcgaagacg acaacctcgg tatttattcc ccgttgttcc aggagatggg gcgcgctgcg
    13261 gcggtacaac cggatgagct ggtctttgct gccctgcgtg acgggatcag tgcggcctgc
    13321 tatgacggtc agaacttctt tgataccgaa catccggttt accccaaagt ggatggctcc
    13381 ggggatgcgc agatggtcag caatatgttt gtggcaaaaa ccggctctgt cggcgcacag
    13441 gctgattaca gcggtccggc ctggtatctg cttgactgct cccgcgcggt aaaaccgctg
    13501 atttatcagg atcgccgtaa ggctgaactg gttgcccaga ccaaagtcga tgaaggtcgc
    13561 gcattcaccg ataacgaatt tgtgtttggt gca
//
LOCUS       DAZGLJ010000123        13262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000123 DAZGLJ010000000
VERSION     DAZGLJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.123"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            32..388
                     /locus_tag="ACHK49_003127"
     CDS             32..388
                     /locus_tag="ACHK49_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO9263737.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(439..1335)
                     /gene="yhaJ"
                     /locus_tag="ACHK49_003128"
     CDS             complement(439..1335)
                     /gene="yhaJ"
                     /locus_tag="ACHK49_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO9263738.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1440..2141
                     /locus_tag="ACHK49_003129"
     CDS             1440..2141
                     /locus_tag="ACHK49_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO9263739.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPDAAFQPRTYPKVDILNVILDGEAEYRDSESNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2164..2328
                     /gene="yhaL"
                     /locus_tag="ACHK49_003130"
     CDS             2164..2328
                     /gene="yhaL"
                     /locus_tag="ACHK49_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO9263740.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2540..3850)
                     /gene="cyuA"
                     /locus_tag="ACHK49_003131"
     CDS             complement(2540..3850)
                     /gene="cyuA"
                     /locus_tag="ACHK49_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO9263741.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3878..5209)
                     /locus_tag="ACHK49_003132"
     CDS             complement(3878..5209)
                     /locus_tag="ACHK49_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9263742.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5484..6848)
                     /gene="tdcG"
                     /locus_tag="ACHK49_003133"
     CDS             complement(5484..6848)
                     /gene="tdcG"
                     /locus_tag="ACHK49_003133"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO9263743.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6920..7309)
                     /locus_tag="ACHK49_003134"
     CDS             complement(6920..7309)
                     /locus_tag="ACHK49_003134"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO9263744.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7323..9617)
                     /gene="tdcE"
                     /locus_tag="ACHK49_003135"
     CDS             complement(7323..9617)
                     /gene="tdcE"
                     /locus_tag="ACHK49_003135"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO9263745.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVTTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9651..10859)
                     /gene="tdcD"
                     /locus_tag="ACHK49_003136"
     CDS             complement(9651..10859)
                     /gene="tdcD"
                     /locus_tag="ACHK49_003136"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO9263746.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGSSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10885..12216)
                     /gene="tdcC"
                     /locus_tag="ACHK49_003137"
     CDS             complement(10885..12216)
                     /gene="tdcC"
                     /locus_tag="ACHK49_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO9263747.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12238..13227)
                     /gene="tdcB"
                     /locus_tag="ACHK49_003138"
     CDS             complement(12238..13227)
                     /gene="tdcB"
                     /locus_tag="ACHK49_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO9263748.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
BASE COUNT         3369 a         3608 c         3158 g         3127 t
ORIGIN      
        1 tttcgtcact tttaattaaa gggatgtttt tatgcagtgg tatttagccg tattaaaaaa
       61 ttatgttggt ttctctggtc gcgcacgtcg taaagagtac tggatgttta ctctgattaa
      121 cgccatcgtc ggcgctatta tcaatgtcat tcaattgatt ttaggtttag agtttccatt
      181 cctttctctc atttacctgg cagcaacaat cattccggtc attgctctgt gtgttcgtcg
      241 tctgcacgat actgaccgtt caggtgcgtg ggcgcttctc tatctggtac cgatcattgg
      301 ctggttagtt ctgtttgtat ttgcctgttt agaaggcaat tctggcagca atcgttacgg
      361 aaacgatcct aagtttggtt caaattaatc ttagaattgg ggcgacattt cgcccctttt
      421 tattaataat aacaaatatt attttccgct aaaaagtttg ggaatttccc gcagacacca
      481 ggatttcgct tcccccatac tgtcacgacg ccaggccata ataatatcga tctcgctggt
      541 cgattccggg ctgacgacac gcaaccgccc ttccgcaata tctttttcga ccatcggata
      601 cggcatcgtc gccacgccaa gccccgccag taatgcctga cgtttatctt caatcgtgct
      661 caccgttaag cgcggctgtt tgtccagcag ctgtacggtc aacaccgggc gctcacgagc
      721 ggtatccgct accgcaattc cacgatattt cacgcgcgtc acttcagata acggttccgg
      781 ctcctgatga atcgggtgat caggcgcggc aacgtagacg ttcattaacg tatagagctt
      841 gcgcgagttg atctccgacg aggaacgaaa atgcatatcc ggcgcgataa caatatccgc
      901 ccgcccctgc tccagtcgtt cccacgcccc cgccagcact tcggtgatga ttgccagttg
      961 ggtattggct tttgccgcca gtttgtcgat taacgggaaa aaggcaggtg tcggtaccag
     1021 cgcttcggtc acaatggtga gatgcgtttc ccaaccgcgc gcgagggctt ccgcatcggt
     1081 agtcagttta tctgcggctt ccagcaaaac gcgccctcgc tccagcaaca tcctcccgac
     1141 attggtgaat ttggtgcgat ggcccgagcg gtcaaacagc accacatcca gctcttcttc
     1201 cagtttttgc atggtgtagc taagtgcaga aggcacgcgt cccagctcat ccgccgccgc
     1261 cgcaaaactg ccccggcgat cgatcgcatc cataacccgt agtgcttcca gcgttaatgc
     1321 cctttctttg gccatttcgt tctcattcag gaaatttgaa cataccgggc agaatatctg
     1381 gctaacaatg tagcgtccag ccccttacca taaaaggaag taaagagagg tcaagaatta
     1441 tgattactac ccgaactgcc aggcagtgtg gacaagcaga ctacggatgg ttgcaggccc
     1501 ggtatacttt ttcctttgga cactacttcg acccgaaatt gttaggctat gcctccctgc
     1561 gtgtgcttaa ccaggaagtg ctggccccag atgccgcctt tcagccgcga acctatccca
     1621 aagtcgatat tttaaatgtg attctggatg gagaagcaga gtatcgcgac agcgaaagca
     1681 atcatgttca ggccagcgcc ggtgaggcgt tgctgctctc tacccagccg ggtgtcagct
     1741 acagcgaaca caatctcagc aaagacaaac cgttaacacg aatgcagctt tggctggacg
     1801 cctgcccgca gcgagagaat ccgctgattc aaaaactggc gcttaatatg ggcaagcagc
     1861 aattaatcgc ctcgccagag ggtacgatgg gaagcctgca attacgccag caagtgtggc
     1921 tgcaccatat cgtgctcgac aaaggcgaaa gtgcgaattt ccagttgcat gggccacgcg
     1981 cgtatttgca atcgattcac gggaaatttc atgcgcttac gcatcatgaa gagaaagcgg
     2041 cgctgacctg cggtgatggg gcgtttattc gtgacgaggc taacattacg ctggttgccg
     2101 attccccact gcgcgctttg ctgatagatt tgcctgtcta gttgttttta cagggagatg
     2161 atgatgagta aaaaatcggc caaaaagcgc cagccagtga agcccgtggt ggcgaaagaa
     2221 cctgctcgca ccgccaaaaa ttttggctat gaagagatgt tgagcgagct ggaagccatc
     2281 gtcgcggatg ctgaaacgcg tttagccgag gatgaagcta ccgcgtaaag acaggttgcc
     2341 ggatgcggcg aaaacgcctt atccggccta caaaatccaa caacattaac gaattaaaca
     2401 acccgtaggc cggataagat gcgccagcat cgcatccggc atcaggtaca aaacacaaca
     2461 acatcaacaa gttgagcacc ccgtaggccg gataaggtgc gccagcatcg catccggcac
     2521 gatacccaaa acctggcgtt tatctggcct tgctcgccat aatctcgata atctgccgat
     2581 ccgtttgctg catcgaatgg cttgctaacg cacacaggtt ggcaatcgac tgctcaacat
     2641 catgcgccac gatcccttca ttgccggtca cggcggtatc atccagcgcc attaacaccg
     2701 ctttccacgc agccgaagca ctggtcgaaa ccttcatcgc gcagctgttc gacgcaccat
     2761 cgcaaatcat gccgctgaca tcgccgatca tactgctgat cgccatcgag atggtttcat
     2821 aacgcccatc caccagccat gccatcccgg cggcggcccc cattgctgcg gtcgttgcgg
     2881 cacacagcgc agacaaacgc ggtaactggt tatggatgta aattgcgctc aaatgagaaa
     2941 gcatcagcgc acgcgccagc cgttcatcat ccgctccgaa gtgttctgct accaccacca
     3001 caggcattgt tgcggtgatc ccctggttac ccgagccgga gttactcata gccggaagcg
     3061 tagcgccgcc catacgcgca tcggatgccg cgctggtacg aatcacaatg gatgaagaga
     3121 gatctttcgc cagcaaaccg cgctcgcact gtttttccag cgtcgcgcca atatgcagcc
     3181 cccacttacc gctcaaacct tcctgcgata acgcacaatt tagcttcgcg gaatcgagaa
     3241 taaagcggat cgccgcaaac gggacttcat tgacgaactt caggatctca gccagcgtcg
     3301 ttctggaaag caccgtcagc ggagactctt gctcgccctc tgccacacac gcctgctggg
     3361 taaacaccac accattgtgc gtctcaatat gcacaatgtt ggtatgcccg ccgacgatgg
     3421 tgacacacgc ccacttctca ccgttccaga ctttggcgcg tgagaagagg atttcattgc
     3481 aaggttcctg gatcttaacg gagactttcc ccgccgccag cagtgctttg gcatcggcaa
     3541 ttgcctgcgc agttgcgtct ttcagcactt ccagcccggc gttggcattt ccacctaacg
     3601 cccccagcgc cgccgcaatc ggcagcccca ccattcccgt gccgggaacg gtgacgccca
     3661 gaccgttctt catcagattt ggcgaaaccc aggcttctac acgttcaacc ggaccttcca
     3721 gttctgccgc agcaaccgcc gccgccagcg ccagtgaaat cggttcagta catcccagcg
     3781 ccggttttac ttcctcctga acggcgagga tgtaacgctg ccataacgga tttaaagtcg
     3841 aatcaaacat attaaaaacc ttaaaatttc aggtaaatca agaaaatgcc aggaacggag
     3901 aaacacaaag caacaaacca gtgacgataa tcaggtacag agacatccct ttgtatttgt
     3961 gcaatgccgg tactttgtaa accagccacg ccgggatcag gcaccctacc atgccgaaaa
     4021 tcgggctaca gatagaggtg aagctcaaca ccggtgcgtt cagtacgatg gcgctccagg
     4081 ccagcaaaat ggcgaaaatc atgatgccgc gctgaacaag attttcgtta atcttctcgg
     4141 caggcatctt gcgacgcagg atgttcatta cgatcccttg cgttgcttcg cgaaagccta
     4201 aatagacgcc aaagaatgca gtcatgacag caaagatatt gagaatgacg ctgaccactt
     4261 tcacccacgc tgcgccgtca ccgctaataa actgtgcggc aatcgccagc gcggaaatat
     4321 tctgctcata ggcttttacc gcttcgtcat gtcccatcgc cagcgtgaac gacacggcgt
     4381 agaaaaagac ggtgacaaac aaaatgccaa acgcgatatt cattgcccgc aatgctttat
     4441 gccgcgccac ttcaattgat ttttcccgcg agcgataaga gatcaccatc gggcttaacg
     4501 tctggataaa cagaatcgac gtcagggtaa acggcagcgt aataatggcg tttttcacca
     4561 gcagccccag cggcggtagc gaaccgacgt tatacagatg ccacattccc accatcgaca
     4621 cgcccagcgc cgcgaccacc agcagcttgg tcagcaccat gccggtcgaa attttgaata
     4681 acaatttctc gccgcgtgaa gagatcgcca ccagaatgca aatcagtacc agaccataaa
     4741 agggactgtc tgacagcaac ccttccgtca cgccgaaggt atgcagatag gaagcactat
     4801 cgttggtgat ggcggtggaa tagacgaaca tccaaatcac cagcattacg aaatagagcg
     4861 cacctaacag gatgccccag tttttaccta aataaccgct aatgacactc gggtaatctt
     4921 tacattctgg tgattctgcc agcgtattaa taaacaaccg ctgaaacaga tacattgccg
     4981 ggtaaccaat caccgatgag agcaaaaata cccacaatcc catcaaaccg acctgcaccg
     5041 ggagaaaaac aatccccgcg ccaatcgcca tcccgatact cataatcacc cagccggtgt
     5101 cagtactgtc gaatttgatc gcttctcgcc actcgctctc actcattcca gcacgacccg
     5161 ccggggtcga agcgtctgca atgacgcctt tattcgatgc aatttccata atttctcgct
     5221 caatattttg tagggcttat tatttttttc cgagccgcat caaggcgata tgcggtctac
     5281 gtttttgcag gcgagttatt agaataaaga aatgatacgc gcgaggtcag agaaaatctt
     5341 cacaatcaag actttgaaat aacggagatg gataagaatt ttctactaaa ttaatcgcaa
     5401 gagaatattc attctctatt tgcggcagat cacaaaaaaa aggtgcacat ttgtgcaccc
     5461 aaggatgaaa gctgacagca atgtcagccg cagaccactt taatggccag tcctccgcgt
     5521 gatgtttcgc ggtatttatc gttcatatct ttgccggttt catacatcgt ctcgatcact
     5581 ttatcgagtg aaacacgcgg tgccgaggtg cggcgcatcg ccatccgcgc ggcgtttact
     5641 gctttcacgg cattaatggc attacgttca atgcacggga tttgtacctg tccggcaacc
     5701 ggatcgcagg tcagcccaag gttatgctcc atcgcgattt ccgccgcatt gcatacctgc
     5761 gccggactgc cgcccagtag ttcagttaac cctgccgccg ccattgaaca ggccacgcca
     5821 atctccccct gacagccgac ctccgcgcca gagatggagg cgttcatttt atacagcgcg
     5881 ccaatagccc ccgctgccag aaaatagcgg gcaattgacc gctcgtttac cggacgacgg
     5941 aacttatcgt aataagccag cactgccgga ataatgccgc acgcaccgtt agtcggtgcc
     6001 gttaccacgc gcccgccagc tgcgttttct tcactaaccg ccagcgcgta catgttgatc
     6061 cagtcgatga cattcatcgg atcgttagag atgttatcgc tggaaaccag ctgacgacgc
     6121 agcgctacgg cacgacgcgg cacattgagc ggaccaggca gcacgccttc agtgttcatc
     6181 ccgcgttcaa taccgtcatg catcacttgc cagatacggg caaaccctgc gtcaatttcc
     6241 gctttgctgc gcagcgccag ctcgttgtgc atcatcaggc cagatataga caggccgttg
     6301 taatcacaca ttttcagcag ttcacctgct gagtggaaat cgtaaggtac ggacgtttcg
     6361 acatcgtgcg acaggccgaa gtgttcttct tcgacaataa acccgccgcc gacagagtaa
     6421 taggttttac ttaatagcgc ttcctgtccc ttccaggcag tgatccgcat tccgttctca
     6481 tggcgaggca acatttcggg atggaagata atgttctttg ctacaggaaa atcaacaata
     6541 tgcgcaccag atgccactgg cagccgcccg ctgcgcgtta ctaactctat aaatgcaggg
     6601 atctcatcaa tgacaacatc ctgcggactg tttcctgcca gtcccatgat aatggcgaca
     6661 tccgtggcat ggcctttgcc cgtcagtgac aacgacccat acagatcgac cacaatatgg
     6721 ctcgtcgcgg ttaataagcc gctactttcc agccgatcaa taaaactttt tccggcattc
     6781 attggcccca cggtatgcga actggaggga ccaatcccaa ttttgaaaat atcgaatgca
     6841 ctaatcatat ccacaccctc ggattgccgt tcagtgaagt ggagcggaac gaccttgcga
     6901 ccgtcccgct cacgaggctt tacgcactac gtactgcgat agcttcaatt tccagcttca
     6961 catctttcgg caaacgcgca acctgcacac agctccgggt cgggtaggtc gcctgatgct
     7021 catcgaagaa ctgcttatag acttcattga tggtggcaaa atcattcaga tcggtgatga
     7081 acacggtcat cttgatgata tcgcccacgc tcagcccggc agcaaccacg atcgctttga
     7141 cgttttcgag gcttaaacgc gcctgatctt gcacatcagc cgggatctca ccggtctgcg
     7201 ggcaaaccgg aatttgcccg gaggtgaaga ccatgctgcc taaatcaacg ccctgaacat
     7261 aagggccgat tgcgcctggg gcacgttgcg tttcgataat ctttttcata catcctccgg
     7321 cgtcagagcg cctgggtaaa agtacgtgaa ataacatcct gttgctgttc acgggtcagt
     7381 gcgttgaagc gcacggcgta gccagagaca cggattgtca ggttaggata tttttccggg
     7441 tgctcgatgg catccagcag catttcccga ttcattacgt tgacgttgag gtgttgaccg
     7501 ccttcgacat ccgcttcgtg gtggaaatac ccatccagca ggccgacaag gttggtttta
     7561 cgtactggat cttctttgcc cagcgccgca ggaacgattg agaaggtgta cgagatccca
     7621 tctttggcgt aggtgaacgg cagtttcgcc accgacgtca atgaggccac ggcacctttg
     7681 cggtcacgac catgcatcgg gttagcgccc ggcgcgaacg gtgttccggc gcgacgaccg
     7741 tccggcgtat taccggtttt ctggccgtac accacgttag aagtgatagt cagaatcgac
     7801 tgggtagtga cggcgttgcg ataggttggc agcgctttaa ttttcttcat aaagcgttca
     7861 accaggtcgc aggcaatgct gtctacgcgc tcgtcgttgt tgccgtactg cggatattca
     7921 ccgtcgattt caaagtccac cgccaggccg ttttcgtcac ggattggttt cacgcgggca
     7981 tatttgatgg cagacaggga gtccgtcgcc accgacaggc ccgcgatgcc gcatgccata
     8041 gtgcgataga catcacgatc gtgcagcgcc atcagcgaag cttcgtagct gtacttgtcg
     8101 tgcatgtagt gaatgatatt cagcgcgctg atgtactgca ccgccagcca gtccatgaag
     8161 tgatcgaggc tgtccatcac tttgtcgtaa tccagcacgt cgtccatcag cggtgctgtt
     8221 ttcggcccga cctgaatctt cagcttctcg tccaccccgc cgttaattgc gtagagcagc
     8281 gttttcgcca ggttagcgcg tgcaccaaag aactgcattt gcttaccaat caccattggg
     8341 ctgacgcagc aggcaatcgc gtaatcgtcg ctgttgaagt cagtacgcat cagatcgtca
     8401 ttttcatact gcaaggaaga ggtgacgatc gacacctgcg cggcatattt tttgaaggca
     8461 atcggtaatt cttccgacca aagaatggtc aggttaggtt ccggtgccgg ccccatagtg
     8521 tgcagggtgt gcaaatagcg gaaggagttt ttggtcacca gcgtacgacc gtccagcccc
     8581 atcccgccga tcacttccgt cgcccagatt gggtcgccgg agaacagcga atcaaattcc
     8641 ggtgtacgca ggaagcgtac catacggatc ttcatgatga agtgatcgat cagttcctgt
     8701 gcctgctgct cattgagtac gccagcttta aagtcgcgct caatgtagat atcgaggaac
     8761 gatgccgtgc ggcccagcga catcgcgccg ccattttgcg atttcactgc cgccagataa
     8821 gcgaagtaga gccactgcac cgcttcctgc gcattctgcg ccgggcgaga gatatcaaag
     8881 ccatatttcg ccgccatttc ctgaatctgc aacagcgcat gacgatgctc tgccagctcc
     8941 tcacgcagac ggatggtggc ttccagatcc tcgccttttt ccagacgaga ctggagatcg
     9001 gcaaattgca gttcgcgttc acgtaccaga taactgatgc catacagcgc tacgcggcga
     9061 tagtcaccga taatgcgccc acggccatag ccatctggta aaccggtcag cacgccagat
     9121 ttacggcagc gcagcatatc cggtgagtaa acatcaaata cgccctggtt atgggtttta
     9181 cgcagatcgg taaacagata ttcaaattca ctgtccattt cacggccata ggcgtggaat
     9241 gaacttttaa tcatattaat gccaccgaac gggtgtagcg cacgtttcaa cggcgcatcc
     9301 gtttgcaggc caacaatttt ttccagcggc tggttaatat atcccgcatc atgagcggta
     9361 attgtggtgg caatattggt atcgaaatca accggcgcgt gggttgcgtt ttcgatacgg
     9421 ataccttcca ttactttttc ccacaattcc gtggtggcag gcgtcgcttc ggcgaggaaa
     9481 gattcatcgc cttcatacgg tgtatagtta tgttgaataa aatcgcggac attaatttcg
     9541 tttttccagt ccgtaccttt aaagccaagc catgcgtcgg cgtacagctt atcgctggta
     9601 tcaatatcta ccttcatgaa aaataatctc tctacaatac ttcaactaaa ttatgcaaat
     9661 tctgcgggcg cgttaacttt gcctaaatga atggcatcca aagcaatcat tttttcttcg
     9721 ttagtcggaa taacagcgca aatgacacgc gcattttcac tggaaacaat tcgctcacca
     9781 caggagttag agcgattatt catttctgta tcaatctcta cgcctaatac agccaaatgt
     9841 tccatgacca gacgacgaat taagcttgaa ttctctccta ttccgccggt gaatataatt
     9901 ccatccaggc gacgtaatga agctgcgtgt ccggcaatat gacgggcaat tcggtgaaca
     9961 aaggttttaa ttgccagttg cgcgcgttcg tgaccttcat gccaggcttt ttccagaaca
    10021 cgtaaatccg aagaaagacc ggaaatacct aataatcccg actctttatt cactacgcgt
    10081 tccaggtcac ccaggctctg gttggtttgg ctggcgaccc aggacatcgc accaaagtcg
    10141 acatcgccac tgcgggtacc catcatcaag ccttccagcg gcgtcattcc cattgaagta
    10201 tcaacactct gaccgttgcg aaccgcgcag attgacgcgc cattgccaag atgcgccaca
    10261 accaggccgg agtcatcttc cgccagattc agcagcgaat gtgcgcgctg ggaaacatag
    10321 cggtgcgacg agccgtggaa accataacgg cgtacaccta actcttcata atatttccac
    10381 ggcaggccgt ataaataagc ttccggagcc atcgtctggt ggaaactggt atcaaatacc
    10441 gccacctgag ttacgcccgg aaataattgc tgcgccgatt caataccact taaattggcg
    10501 taattatgca ggggtgccag tggagaaacg cgacggatat tatcaatgac ttcatcggta
    10561 ataatggcgg actcggtaaa aatactgccg ccgtgagcga tgcggtggcc aattaaggcc
    10621 acactgtcat ttaaattccg tttttccagt tcaaatgcaa ttgccttcaa tgcaccttcg
    10681 tagctgtggt gagccagcgg tgctggctct cccccattta cggataagaa tgcattttcc
    10741 gagttaatac cgtcggcaat acctgacatt aatacttcac agtcgctggc atcgagtacg
    10801 gaaaacttaa tcgaagacga accacagtta ataaccaaaa caaccggaaa ttcattcatc
    10861 tcttttctca tcctgagtta cggattaaaa cagtttgtat acgatgttca ggatggtcag
    10921 cagaccaatc acggtaacaa acacgttatc cagacgacca cggtatttcg ccagagacgg
    10981 cgctttacgg atggcataca tcggcaacag gcacagcagg gatgcgataa tcggtgcgcc
    11041 catggcttca ataaggtcga ggatgttcgg gttggcgtag gcaacaaccc aggtggagcc
    11101 catgatgaag atcatgctga tagtgttcag tttacccagc gacactttag ttttgtcgcc
    11161 tttataaccg aacttcagaa tcagaccatt caagccttcc agcgtcccca gatagtgacc
    11221 gaagaaagat ttgaagatag ccacgagtgc gatgatggaa gccgcatatt ccagtgtaat
    11281 cgcgaacgtt gttttggtac cggtcatgga cgcaaagtgg ttagccagat aagaaagcac
    11341 tggaatattc tgcgctttgg cttccgccat gttggccgga gacagcgtaa acaggcagct
    11401 aaaggcaaag aacatcacca ctgcaaccat cagcatgctg gcacgagaaa tgatttggga
    11461 acatttacgt tcggtgaagt cgcgaccgaa gtctttctca tactcttcac gtttagaaac
    11521 cacgaaggaa gagacgattg gcgagaagtt aaaggagaaa accatgatgg aaatccccag
    11581 ccagacagtg atcaggatac cgtcatgacc ggttaacgac agcgaaccga ggtcaacctg
    11641 gtcgataact gcagagttcc agtaagggat cagcgacaga gaaatcagca ccaggctggc
    11701 gataaacggc cataccaggt agctcatcac tttaaccatc agatccttac caaaccagat
    11761 gacgaaagcc atcagcagca acaggaacag cgccacaaag ccgcgattca gcggtgcaaa
    11821 tccgagctgg ttttcccaga acgtcataaa ggtattggta atagtaacgc cataaatcca
    11881 cagcagtggg caaatcgcga agaagtacag gaacgtgata accacgccgc cagttttacc
    11941 aaaatgctct tccaccgttt ccgtaatgtt gccggaaggg ttagagccag aaagacacag
    12001 acgcgccagc gcccggtggc aataaaatgc gatggggtat gccaatacca acatcagaag
    12061 aatcgggatc agtccgccaa aacctgcgcg gatagggaag aacagcaccc cggcgccgat
    12121 tgccgtacca aacaagccta acgtccatgt ggtatctgat ttacgccagg acgattgttt
    12181 tgtctggctg gatacaatgc tatctgaagt actcatatcc tatcctcaac gaattaatta
    12241 agcgtcaacg aaaccggtga tttgagagac gcgagaaaga tcgatattgc cgccggaaat
    12301 aatactgacg gtttttctgt tttgaatata ttggtctaat ttaccgctta ataatgcagc
    12361 acatgccaga gcgcctgcgc cttcggtgac gactttattg cgctgaatta aggcaatcat
    12421 actgtttctg atttcgtctt cgctgaccag cacgatgtca tcgactaatt cacgaacgat
    12481 ttcgtaagtt aaattacccg ggcgggagac atcacaacca tccgccaggg tgccggtagt
    12541 tcggtgcgtg gttatttctc cggagtggaa agaagccgcc atgccgtgaa cgttttcaga
    12601 ctgtacgcca ataacacgaa tggtcgggtt aatagattta attgccaccg caataccagc
    12661 aattaaaccg ccaccaccaa ttggcacaat cacgttatcg acatcataga gatcttccat
    12721 aatttccaga ccaatcgttc cctggccagc aatcactttc ggatcatcgt aaggtgggat
    12781 aaaaatacgg ccttccattt cgacaatttc gctcacttta gcgatagtgt cgttgaaatt
    12841 atcaccatgc agaacgactt ctgcggagta gtcgcacgtt gccgctactt tggattttgg
    12901 cgcacctttt ggcatcacca ctttaccgtc gatacccagc atcgcgcagg agagcgaaac
    12961 cccttgcgca tggttgcccg cagaacaggc cactacacct ttacgttttt ccgcatcagt
    13021 cagtgaactt aatttattaa atgcgccacg aattttaaat gaacccgtac gctgcatatt
    13081 ttcaaacttc aggaatattt cacctttgca acgttcacta aaatagtttg agcgaggcat
    13141 gcctgtttta taaattcgcc cagccagtcg ttgtttcgct tcaataatgt catcaatagc
    13201 aaccggcaga tcgtatgtaa tatgcattat aaaacctctt cgcctgaatt aaatatgtag
    13261 gt
//
LOCUS       DAZGLJ010000124        13144 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000124 DAZGLJ010000000
VERSION     DAZGLJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.124"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            368..1477
                     /gene="livK"
                     /locus_tag="ACHK49_003139"
     CDS             368..1477
                     /gene="livK"
                     /locus_tag="ACHK49_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO9263749.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1525..2451
                     /gene="livH"
                     /locus_tag="ACHK49_003140"
     CDS             1525..2451
                     /gene="livH"
                     /locus_tag="ACHK49_003140"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO9263750.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2448..3725
                     /gene="livM"
                     /locus_tag="ACHK49_003141"
     CDS             2448..3725
                     /gene="livM"
                     /locus_tag="ACHK49_003141"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO9263751.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3722..4489
                     /gene="livG"
                     /locus_tag="ACHK49_003142"
     CDS             3722..4489
                     /gene="livG"
                     /locus_tag="ACHK49_003142"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO9263752.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4491..5204
                     /gene="livF"
                     /locus_tag="ACHK49_003143"
     CDS             4491..5204
                     /gene="livF"
                     /locus_tag="ACHK49_003143"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO9263753.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5600..6916
                     /gene="ugpB"
                     /locus_tag="ACHK49_003144"
     CDS             5600..6916
                     /gene="ugpB"
                     /locus_tag="ACHK49_003144"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO9263754.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            7014..7901
                     /gene="ugpA"
                     /locus_tag="ACHK49_003145"
     CDS             7014..7901
                     /gene="ugpA"
                     /locus_tag="ACHK49_003145"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO9263755.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7898..8743
                     /gene="ugpE"
                     /locus_tag="ACHK49_003146"
     CDS             7898..8743
                     /gene="ugpE"
                     /locus_tag="ACHK49_003146"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO9263756.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8745..9815
                     /gene="ugpC"
                     /locus_tag="ACHK49_003147"
     CDS             8745..9815
                     /gene="ugpC"
                     /locus_tag="ACHK49_003147"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO9263757.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9812..10555
                     /gene="ugpQ"
                     /locus_tag="ACHK49_003148"
     CDS             9812..10555
                     /gene="ugpQ"
                     /locus_tag="ACHK49_003148"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO9263758.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLWILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10542..10982)
                     /locus_tag="ACHK49_003149"
     CDS             complement(10542..10982)
                     /locus_tag="ACHK49_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO9263759.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            11102..12844
                     /gene="ggt"
                     /locus_tag="ACHK49_003150"
     CDS             11102..12844
                     /gene="ggt"
                     /locus_tag="ACHK49_003150"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO9263760.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12882..>13144)
                     /locus_tag="ACHK49_003151"
     CDS             complement(12882..>13144)
                     /locus_tag="ACHK49_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO9263761.1"
                     /translation="AKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFLETGDEAAR
                     LAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2918 a         3352 c         3755 g         3119 t
ORIGIN      
        1 aggctttctc ttttttattt cttttggcag gtgattaatt ttttaacagc aataattaca
       61 aagttaaaac attagagaat gaaaaatacc cagcataatc ccctgaatgg tagtgaatta
      121 ttccgccctt tgtgccgtta ttttatgctg acaaaggcac ttttttctgt ttatctatca
      181 ataaattcag aatattatct gttcttaatc gactgaaaaa tagggatttt aatcactatt
      241 atcacaaaat actgcgctaa acccttaatc agacaggcaa aaacagtgca gtataaaaaa
      301 agaacagtct gatttgttaa cacataaaaa caaagcaaca caacatcacg aatggggatt
      361 tttgactatg aaacggaatg cgaaaactat catcgcaggg atgattgcac tggcaatttc
      421 acacaccgct atggctgacg atattaaagt cgccgttgtc ggcgcgatgt ccggcccgat
      481 tgcccagtgg ggcgatatgg aatttaacgg cgcgcgtcag gcgattaaag acattaatgc
      541 caaaggggga attaagggcg acaagctggt tggcgtggaa tatgacgacg cctgcgaccc
      601 gaaacaagcc gttgcggtcg ccaacaaaat cgttaatgac ggcattaaat acgttattgg
      661 tcatctgtgt tcttcttcta cccagcctgc gtcagatatc tatgaagacg aaggtattct
      721 gatgatctcg ccgggagcga ccaacccgga gctgacccaa cgcggttatc aacacattat
      781 gcgtactgcc gggctggact cttcccaggg gccaacggcg gcaaaataca ttcttgagac
      841 ggtgaagccc cagcgcatcg ccatcattca cgacaaacaa caatatggcg aagggctggc
      901 acgttcggtg caggacgggc tgaaagctgc taacgccaat gttgttttct tcgacggcat
      961 taccgctggc gagaaagatt tctccgcgct gatcgcccgc ctgaaaaaag aaaacatcga
     1021 cttcgtttac tacggcggtt actacccgga aatggggcag atgctgcgcc aggcccgttc
     1081 cgttggcctg aaaacccagt ttatggggcc ggaaggtgtg ggtaatgcgt cgctgtcgaa
     1141 tattgctggc gatgctgccg aaggcatgtt ggtcaccatg ccaaaacgct atgaccagga
     1201 tccggcaaac cagggcatcg ttgatgcgct gaaagcagac aagaaagatc cgtccgggcc
     1261 ttatgtctgg atcacttacg cggcggtgca atctctggca acagcccttg agcgtaccgg
     1321 cagcgatgag ccgctggcgc tggtgaaaga tttaaaagct aacggtgcaa acaccgtgat
     1381 tgggccgctg aactgggatg aaaaaggcga tcttaaggga tttgattttg gtgtcttcca
     1441 gtggcacgcc gacggttcat ccacggcagc caagtgatca tcccaccgcc cgtaaaatgc
     1501 gggcgggttt agaaaggtta ccttatgtct gagcagtttt tgtatttctt gcagcagatg
     1561 tttaacggcg tcacgctggg cagtacctac gcgctgatag ccatcggcta caccatggtt
     1621 tacggcatta tcggcatgat aaacttcgcc cacggcgagg tttatatgat tggcagctac
     1681 gtctcattta tgatcatcgc cgcgctgatg atgatgggca ttgataccgg ctggctgctg
     1741 gtggcggcgg gattcgtcgg cgcaatcgtc attgccagcg cctacggctg gagtatcgaa
     1801 cgggtggcct atcgcccggt gcgtaactct aagcgcctga ttgcgctcat ctcggcaatc
     1861 ggtatgtcca tcttcctgca aaactacgtc agcctgaccg aaggttcgcg tgacgtggcg
     1921 ctgccgagcc tgtttaacgg tcagtgggtg gtgggccata gcgaaaactt ctctgcctct
     1981 attactacca tgcaggcggt gatctggatt gtcaccttcc tcgccatgct ggcgctgacg
     2041 attttcattc gctattcccg tatggggcgc gcgtgtcgtg cctgcgcgga agatctgaaa
     2101 atggcgagtc tgcttggcat taacaccgac cgggtgattg cgctgacctt tgtgattggc
     2161 gcggcgatgg cggcggtggc gggtgtgctg ctcggtcagt tctacggcgt aattaacccc
     2221 tacatcggct ttatggccgg gatgaaagcc tttaccgctg cggtgctcgg tgggattggc
     2281 agcattccag gggcgatgat tggtgggctg atcctgggga ttgcggaggc gctctcttct
     2341 gcctatctga gtacggaata taaggatgtg gtttcatttg ccctgctgat tctggtgctg
     2401 ctggtgatgc cgaccggtat tctgggtcgc ccggaggtag agaaagtatg aaaccgatgc
     2461 atattgcaat ggcgctgctc tctgccgcga tgttttttgt cctggcgggc gtctttatgg
     2521 gcgtgcaact ggagctggat ggcaccaaac tggtggtcga cacggcttca gacatccgtt
     2581 ggcagtgggt gtttatcggc acggcggtgg tctttttctt ccagcttttg cgaccggctt
     2641 tccagaaagg gctaaaaagc gtttccggac cgaagtttat tctgcccgcg attgatggct
     2701 ccacggtgaa gcagaaacta ttcctcgtgg cgctgctggt gcttgcggtg gcgtggccgt
     2761 ttatggtgtc gcgtgggacg gtggatatcg ccaccctgac catgatctac attatcctcg
     2821 gcctcgggct gaacgtggtt gttggtcttt ctggtctgct ggtgctgggg tacggcggct
     2881 tttacgccat cggcgcttac acttttgcgc tgctcaatca ctattacggc ctgggcttct
     2941 ggacctgtct gccgattgcc gggttaatgg cagcggcggc cggcttcctg ctcggcttcc
     3001 cggtgcttcg cttgcgcggc gactatctgg cgatcgttac cctcggtttc ggcgaaattg
     3061 tgcgcatatt gctgctcaat aacaccgaaa ttaccggcgg cccgaacgga atcagtcaga
     3121 tcccgaaacc gacactcttc ggactcgagt tcagccgtac cgctcgtgaa ggcggctggg
     3181 acacgttcag taatttcttt ggcctgaaat acgatccctc cgatcgtgtc atcttcctct
     3241 acctggtggc gttgctgctg gtggtgctaa gcctgtttgt cattaaccgc ctgctgcgga
     3301 tgccgctggg gcgtgcgtgg gaagcgttgc gtgaagacga gatcgcctgc cgttcgctgg
     3361 gcttaagccc gcgtcgtatc aagctgactg cctttaccat aagtgccgcg tttgccggtt
     3421 ttgccggaac gctgtttgct gcgcgccagg gctttgtcag cccggaatcc ttcacctttg
     3481 ctgaatcggc gtttgtactg gcgatagtgg tgctcggcgg tatgggatcg cagtttgcgg
     3541 tgattctggc ggcaattttg ctggtggtat cgcgcgaatt gatgcgtgat ttcaacgaat
     3601 acagcatgtt aatgctcggt ggtttgatgg tgctgatgat gatctggcgt ccgcagggct
     3661 tgctgcccat gacgcgcccg caactgaagc tgaaaaacgg cgcagcgaaa ggagagcagg
     3721 catgagtcag ccattattat ctgttaacgg tctgatgatg cgcttcggcg gcttgctggc
     3781 ggtgaacaac gtcaatcttg aactgtaccc gcaggagatc gtctcgttaa tcggccctaa
     3841 cggtgccgga aaaaccacgg tttttaactg cctgaccgga ttctacaaac ccaccggcgg
     3901 cactatttta ctgcgtgatc agcatctgga aggtttaccg gggcagcaaa ttgcccgcat
     3961 gggcgtggtg cgtaccttcc agcatgtgcg tctgttccgt gaaatgacgg tgattgaaaa
     4021 cctgctggtg gcgcagcatc aacaactgaa aaccgggctg ttctctggcc tgttgaaaac
     4081 gccttctttc cgtcgcgccc agagcgaagc gctcgaccgc gccgccacct ggcttgagcg
     4141 cattggtttg ctggaacacg ccaaccgtca ggcgagtaac ctggcctatg gtgaccaacg
     4201 ccgtctggag attgcccgct gcatggtgac gcagccggag attttaatgc tcgacgaacc
     4261 ggcggcaggt cttaacccaa aagaaaccaa agagctggat gagctgattg ccgaactgcg
     4321 caatcatcac aacaccacta tcttgttgat tgaacacgat atgaagctgg tgatggggat
     4381 ttcagaccga atttatgtgg tcaatcaggg gacgccactg gcaaacggta cgccggagca
     4441 aatccgtaat aacccggacg tgatccgtgc ctatttaggt gaggcataag atggaaaaag
     4501 tcatgttgtc ctttgacaaa gtcagcgccc actacggcaa aatccaggcg ctgcatgagg
     4561 tgagcctgca tatcaatcag ggcgagattg tcaccctcat tggcgcgaac ggggcgggga
     4621 aaaccacctt gctcggcacg ttatgcggcg atccgcgcgc taccagcggg cgaattgtgt
     4681 ttgatgataa agacattacc gactggcaga cggcgaaaat catgcgcgaa gcggtagcga
     4741 ttgtcccgga agggcgtcgc gtcttctcgc ggatgacggt ggaagagaac ctggcgatgg
     4801 gcggcttttt tgccgaacgc gaccagttcc aggagcgcat aaagtgggtg tatgaactgt
     4861 ttccacgtct gcatgagcgc cgtgttcagc gggcaggcac catgtccggc ggtgagcaac
     4921 aaatgctggc gattggtcgc gcgctgatga gcaacccgcg tttgctgcta cttgatgagc
     4981 catcgctcgg tcttgcgccg attatcatcc agcaaatttt cgacaccatc gagcagctgc
     5041 gcgagcaggg gatgactatc ttcctcgtcg agcagaacgc caaccaggcg ctaaagctgg
     5101 cggatcgcgg ctacgtgctg gaaaacggcc atgtggtgct ctccgatacc ggcgacgcgc
     5161 tgctggcgaa cgaagcagtg agaagtgcgt atttaggcgg gtaataacac gttgattgat
     5221 aaggagtcaa aagactcctt tgagacaggt gacaaatgta aaattgcctg atgcgctgcg
     5281 cttatcaggc ctactgggtg agttgcaata tattgaattt gcacgatctt gtaggccgga
     5341 taaggcgttt acgccgcatc cggcatgaaa cgatgggtaa tctgtagagt ttgattcaga
     5401 ccttctattt tccgcttatc cgtgccccat ctcccatttt ccctcaccca cgccgtcacc
     5461 gccttgtcat ctttctgaca ccttactatc ttacaaatgt aacaaaaaag ttatttttct
     5521 gtaattcgag catgtcatgt taccccgcga gcataaaacg cgtgaattcg cgcattcggt
     5581 acaacaagag agataaacga tgaaaccgtt acattataca gcttcagcac tggcgctcgg
     5641 actggcgtta atggggaatg cacaggcagt gacgaccatt ccgttctggc attctatgga
     5701 aggggaactg ggtaaagagg tggattctct ggcccaacgt tttaacgccg aaaatccgga
     5761 ttacaaaatt gtaccgacct ataaaggcaa ctacgaacag aatttaagcg cggggattgc
     5821 cgcatttcgt accggcaacg ctccggctat tttgcaggtt tatgaagttg gcaccgccac
     5881 catgatggca tcgaaagcca ttaaaccggt atatgacgtg tttaaagagg cggggattca
     5941 attcgatgag tcgcagtttg tgccgacggt ttccggttac tactccgaca gcaaaacggg
     6001 ccacttactc tcccagccat tcaacagctc gacccccgtt ctctattaca acaaagacgc
     6061 cttcaagaaa gcaggattag acccggaaca accgccgaaa acctggcagg atctggcgga
     6121 ctatgccgcg aaactgaaag cctccggtat gaagtgcggc tacgccagcg gctggcaggg
     6181 ctggatccaa ctggaaaact ttagcgcctg gaacggtctg ccgtttgcca gcaaaaacaa
     6241 cggctttgac ggcacggacg cggtgctgga gttcaacaag ccggagcagg tgaaacatat
     6301 cgccatgctc gaggagatga acaagaaggg cgacttcagc tacgtcggtc gtaaggatga
     6361 atccaccgag aagttctata acggtgattg cgcgatgacc accgcctctt ccggttctct
     6421 tgccaacatt cgcgagtacg ccaaatttaa ttacggcgta ggcatgatgc cttacgacgc
     6481 cgatgcgaaa gatgcgccac aaaacgccat tatcggcgga gccagcctgt gggtgatgca
     6541 gggtaaagat aaagaaacgt ataccggtgt ggcgaagttc ctcgatttcc tcgcgaagcc
     6601 agaaaacgct gccgagtggc atcagaaaac cggttatctg ccaattacca aagcggcgta
     6661 tgacctgacc cgtgagcagg gcttttacga gaaaaaccca ggtgcggata ccgcgacgcg
     6721 tcagatgctg aataagccgc cgttgccgtt caccaaaggg ctgcgtctgg gcaacatgcc
     6781 gcagatccgc gtgattgtgg atgaagagct ggagagcgtg tggaccggta agaagacacc
     6841 acagcaggca ctggataccg ccgttgagcg tggaaatcag ttgctgcgcc gctttgagaa
     6901 atcgacgaag tcttaatcag tgtaatgtcg gatgcgtttc gcttatctga cttggcatcg
     6961 cgtgtaggcc ggataagcga agcgcatccg gcacagttca ggaattaacc gtaatgtcat
     7021 catcccgtcc ggtgttccgc tcgcgctggc tgccttatct gctggtcgcg ccgcagctca
     7081 tcatcaccgt tatctttttt atctggcctg cgggcgaagc gttgtggtac tcgctacaaa
     7141 gcgtcgatcc gtttggtttc tccagccagt ttgtcggcct ggataacttc gtcgcgctgt
     7201 ttcatgacag ctactatctc gactccttct ggacgacgat aaaattcagc acctttgtca
     7261 ccgtcagcgg tttgctggtg tcgctgttct tcgcggcgct ggtggagtac atcgtgcgcg
     7321 gcagccgttt ctatcaaacc ttaatgctgc tgccttacgc cgtggctccc gccgttgccg
     7381 ccgtattgtg gatcttcctg tttaaccccg gtcgcgggct gatcacccat tttctcgcgg
     7441 agttcggcta cgactggaac cacgcgcaaa acagcggtca ggcaatgttt ctggtggtgt
     7501 ttgcctcagt atggaagcaa atcagctaca acttcctgtt cttctatgcc gcgctgcaat
     7561 ccattccccg ttcgttgatc gaagccgcag ccatcgacgg tgcaggccca attcgccgct
     7621 tctttaagat tgcgctgccg cttatcgctc cggtgagttt cttcctgctg gtggtgaacc
     7681 tggtgtatgc cttcttcgac accttcccgg tgatcgacgc cgccacgtcc ggcgggccgg
     7741 ttcaggccac cacgacgctg atttataaga tctaccgtga aggctttacc ggactggatc
     7801 tggcttcgtc tgccgcgcag tcggtggtgt tgatgttcct cgtcatcgtg ctgacggtgg
     7861 tgcagttccg ctatgttgaa agcaaggtgc gttaccaatg attgagaacc gtccgtggct
     7921 gacgatattc agccatacca tgctgatcct cgggatcgcg gtgatcctct tcccgctgta
     7981 cgtggcgttt gtcgcggcga cgctggataa acaggccgtc tatgccgcgc cgatgacgct
     8041 catccccggc acacatctgc tggaaaatat ccacaacatc tgggttaacg gggtaggcac
     8101 gaatagcgcg ccgttctggc ggatgttgct taacagcttt gtgatggcgt tcagcattac
     8161 gctcggcaaa attaccgtct cgatgctctc ggcatttgcc attgtctggt ttcgttttcc
     8221 gctacgtaac ctcttcttct ggatgatttt tatcaccctg atgctgccgg ttgaagtacg
     8281 tatcttcccg acggtggaag tcatcgccaa cctgaagatg ctcgacagct acgccggttt
     8341 aacgctgccg ctgatggcct cggcgaccgc cacgtttctg tttcgccagt tctttatgac
     8401 gctgccggat gaactggtgg aagcggcgcg gatcgacggc gcgtcaccaa tgcgtttctt
     8461 ttgcgacatc gtttttccgc tctccaaaac caatctggcg gcgctgtttg tgatcacctt
     8521 tatttacggc tggaaccagt atttgtggcc gttgttgatt attaccgatg tggatctcgg
     8581 caccaccgtg gcagggatca aagggatgat cgctacaggc gaaggcacca ctgaatggaa
     8641 ctcagtgatg gcggcgatgt tgttaacgct tatccctccg gtggtgattg ttttagtgat
     8701 gcagcgtgcc ttcgtgcgcg gcctggtcga tagtgagaaa taagatggca ggactgaaat
     8761 tacaggcagt aaccaaaagc tgggatggta aaacccaggt cattaaaccg ctgacccttg
     8821 atgtggcgga tggcgaattt atcgtgatgg tcgggccgtc tggctgcggg aaatcgacgc
     8881 tgctgcgcat ggttgccggg ctggagcggg tgacagaagg cgatatctgg atcaacgacc
     8941 agcgcgtgac tgaaatggag ccaaaagatc gcgggattgc gatggtgttc cagaactacg
     9001 cgctttatcc gcatatgagt gtcgaagaaa acatggcgtg ggggctgaaa attcgcggca
     9061 tgggcaagca gcaaattgcc gagcgcgtta aagaagcggc gcgcattctg gagctggacg
     9121 gtctgctcaa acgtcgcccg cgcgagcttt ccggcggtca gcgccagcgt gtggcgatgg
     9181 gccgcgcgat tgtgcgcgat ccggcggtgt tcctgtttga tgagccgctc tctaacctcg
     9241 atgccaagct gcgcgtgcag atgcgtcttg aactgcaaca gttgcaccgt cgcctgaaaa
     9301 cgacttcact ctacgttact cacgatcagg ttgaagcgat gacgctcgcc cagcgagtaa
     9361 tggtgatgaa cggcggcgtt gccgaacaga ttggcacacc agttgaagtc tacgaaaagc
     9421 ccgccagcct gtttgtagcg agttttatcg gcagtccggc gatgaacctg ctgacaggcc
     9481 gcgtgaataa cgaaggcacg catttcgaac tggacggcgg tattgagctg ccgctaaacg
     9541 gtggctaccg tcagtatgcc gggcgtaaaa tgactctcgg cattcgcccg gaacatattg
     9601 cgctaagctc gcaggcagaa ggcggcgtac cgctggtgat ggacacgctg gagatcctcg
     9661 gcgcagataa cctggcgcac ggacgctggg gcgagcagaa gctggtggtg cgactggcgc
     9721 atcaggagcg cccgacggca ggcagcacgc tgtggctgca tctggcggaa aatcagctgc
     9781 atctttttga tggtgaaaca ggacaacgag tatgagtaac tggccttatc cccgcatcgt
     9841 cgctcatcgt ggcggcggta agctggcccc ggaaaacacc ctggcggcaa tcgacgtcgg
     9901 ggcaaaatac ggtcataaga tgatcgaatt tgacgcgaag ttatcgaaag atggcgagat
     9961 cttcctgctc catgacgaca atctcgaacg caccagcaac ggctggggcg tcgcgggtga
    10021 actgaactgg caggatttac tgcgcgtgga tgcgggcagt tggtacagca aagcgtttaa
    10081 aggtgagccg ctgccgttgc tttcgcaggt ggcggaacgc tgtcgcgaac acgggatgat
    10141 ggcgaatatc gaaatcaaac ccaccactgg cactggacca ttaacgggca aaatggtggc
    10201 gctggcggca cgccaactgt gggccggtat gacgccgccg ctgctgtcat cgtttgagat
    10261 tgatgcttta gaagctgcac agctggcggc accggaactg ccgcgtggtt tattgcttga
    10321 tgagtggcgc gacgactggc gcgaactgac cgcgcggctg ggctgcgtct ctattcatct
    10381 caatcataag ttactcgata aagtgcgagt gatgcagttg aaagacgccg gactgtggat
    10441 tctggtttat accgtcaata aaccccagcg cgcggcagag ttgctgcgct ggggcgtgga
    10501 ttgcatctgt accgatgcga ttgacgtgat tggtccgaac tttacggccc aatagttttc
    10561 aacggaatgt caggctgcgg cgtgctgggc tgattcagca tgtcgccgtt tctcctctcc
    10621 ggcagcatat gctgctgatt caacgaacta tccggattac ggttgctgtt taacatcccg
    10681 ccgttggtgt tgggcagcat ttgctgccgg gcgggatttc gctcccccgg ctgtgactgc
    10741 aacacccgct gagaattatt gtttatctgg ttttctaaat gctgctgttg caactgagtt
    10801 tgcgttttca gttgctgatt cagcatccct ttttgctgga tttgctgagt ctgcatctgg
    10861 gtttgcatcc gctgctggct gggtatctga taccccggct ggttagggtt gttcagagta
    10921 ttaatgggct gtgcaaagcc gacaaacggc aggagtgccg taagaatcag aagccgtttc
    10981 atcgcgtatc ctcctctgaa gatatccttt aagtttactc gcttcccgac aaaacgatga
    11041 ttaattcaga gttatatacc aggcttagct ggggttgccc cttaatctct ggagaataac
    11101 gatgataaaa ccgacgtttt tacgccgggt ggccattgct gctctgctct caggaagttg
    11161 ttttagcgcc gccgccgcgc ctcctgcgcc gcccgtctcg tatggtgtgg aggaagatgt
    11221 cttccacccg gtacgcgcga aacagggaat ggtagcgtct gtggacgcca ctgccactca
    11281 ggtgggggtg gatattctca aggagggcgg gaatgccgtt gatgccgccg tggcggtggg
    11341 ctacgcgctg gcggtaacgc atccgcaggc agggaatctg ggcggtggtg gttttatgct
    11401 gatccgctcg aaaaatggca ataccaccgc tatcgatttc cgcgaaatgg cacccgctaa
    11461 agcgacccgc gatatgttcc ttgatgacca gggcaacccg gacagcaaaa aatcactcac
    11521 ttcgcatctg gcttccggca caccaggtac ggtcgctggt ttctcgctgg cgttggataa
    11581 gtacggcact atgccgttga acaaagtggt gcagcccgcg ttcaaactgg cacgcgatgg
    11641 ttttatcgtt aacgatgctt tggcagacga tctcaaaacc tacggtagcg aagtgttgcc
    11701 gaatcacgaa aacagcaaag ctatcttctg gaaagagggc gaaccgctga aaaagggcga
    11761 cacgctggtg caggcgaacc tggcaaagag cctggagatg attgctgaaa acggcccgga
    11821 cgaattctat aaaggcacga ttgcggaaca gatcgcccag gagatgcaga aaaacggtgg
    11881 cttgatcact aaagaagatt tagcggccta taaagcggtc gaacgcactc cgataagcgg
    11941 cgattatcgc gggtatcagg tttactccat gccaccgcca tcctccggcg ggatccatat
    12001 cgtacaaatc ctcaatattc tggaaaactt cgatatgaag aaatacggct ttggcagcgc
    12061 cgatgcgatg caaatcatgg cagaagcgga gaaatacgcc tacgccgacc gctcggaata
    12121 tcttggcgac ccggattttg tcaaagtccc gtggcaggca ctgaccaata aagcctatgc
    12181 caaatccatt gccgatcaaa ttgatatcaa caaagcgaag ccgtccagtg agattcgccc
    12241 tggcaagctt gcgccttatg agagtaatca aactacccat tactctgtag tggataaaga
    12301 cggtaacgcg gtggcggtga cctatacgct gaacaccacc ttcggtacgg gtattgtcgc
    12361 gggcgagagc ggtattctgc ttaataacca gatggatgat ttctccgcca aaccgggcgt
    12421 accgaacgtt tacgggctgg tgggcggtga tgccaacgcc gtcgggccga acaaacgccc
    12481 gctgtcgtcg atgtcgccga ccattgtggt gaaagacggt aaaacctggc tggttaccgg
    12541 tagcccaggc ggtagccgga tcatcactac agtgctgcaa atggtggtga atagcatcga
    12601 ttatggcatg aacgtcgccg aagcgaccaa tgcgccgcgt ttccaccatc agtggttgcc
    12661 ggacgagctg cgtgtcgaaa aagggtttag cccggatacg ctcaagctgc tggaagcaaa
    12721 aggtcagaaa gtggcgctga aagaggcgat gggcagtaca caaagcatta tggttgggcc
    12781 ggacggtgag ttgtacggcg catccgaccc gcgctcggtg gatgatttaa cggcggggta
    12841 ctaaggttag cggccctctt cgtgggaaga gggctatttt gtcagggcaa gccgaaggta
    12901 gcctttttta tttcgtaatc ctgtagatat tcttccagcg gctttgccgt tcccagagaa
    12961 tgaatttcac cactgtcttt atcaataata aaaggtgcgt taccagctaa gcgcgcggcc
    13021 tcatctccag tttcgagaaa ttctcgtgct tcgaaacaga aataccagcc ctggctaaag
    13081 cgtccatgta gagtaatgac gaccgggaga tctgcatcat caaggtaatg gttcgctttc
    13141 gcga
//
LOCUS       DAZGLJ010000125        13047 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000125 DAZGLJ010000000
VERSION     DAZGLJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.125"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            294..4754
                     /gene="gltB"
                     /locus_tag="ACHK49_003152"
     CDS             294..4754
                     /gene="gltB"
                     /locus_tag="ACHK49_003152"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO9263762.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            4767..6185
                     /gene="gltD"
                     /locus_tag="ACHK49_003153"
     CDS             4767..6185
                     /gene="gltD"
                     /locus_tag="ACHK49_003153"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO9263763.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNPLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            6369..7416
                     /gene="yhcG"
                     /locus_tag="ACHK49_003154"
                     /pseudo
     CDS             6369..7416
                     /gene="yhcG"
                     /locus_tag="ACHK49_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcG family protein"
     gene            complement(7476..7940)
                     /gene="nanQ"
                     /locus_tag="ACHK49_003155"
     CDS             complement(7476..7940)
                     /gene="nanQ"
                     /locus_tag="ACHK49_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO9263764.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(7937..8812)
                     /gene="nanK"
                     /locus_tag="ACHK49_003156"
     CDS             complement(7937..8812)
                     /gene="nanK"
                     /locus_tag="ACHK49_003156"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO9263765.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(8809..9498)
                     /gene="nanE"
                     /locus_tag="ACHK49_003157"
     CDS             complement(8809..9498)
                     /gene="nanE"
                     /locus_tag="ACHK49_003157"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO9263766.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(9546..11036)
                     /gene="nanT"
                     /locus_tag="ACHK49_003158"
     CDS             complement(9546..11036)
                     /gene="nanT"
                     /locus_tag="ACHK49_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO9263767.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(11146..12039)
                     /gene="nanA"
                     /locus_tag="ACHK49_003159"
     CDS             complement(11146..12039)
                     /gene="nanA"
                     /locus_tag="ACHK49_003159"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO9263768.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(12161..12952)
                     /gene="nanR"
                     /locus_tag="ACHK49_003160"
     CDS             complement(12161..12952)
                     /gene="nanR"
                     /locus_tag="ACHK49_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO9263769.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         3098 a         3597 c         3521 g         2831 t
ORIGIN      
        1 gaattttgcg ctaaagcaca tttctgtacc aataagcttg ccatttgacc tgtatcagct
       61 ttcccgataa gttggaaatc cgctggaagc tttctggatg agcagcctgc tcatcatatt
      121 tatgcagtaa ttgagatccc ctcttcaccg tattaaccga tgcgaaaagg acaacaaggg
      181 ggcgaatgcg aggcgcgcgt atgacacgca aaccccgtcg ccacgctctt tctgtgcccg
      241 tgcgcagcgg ttcggaagtg gggttcccgc agagcctggg ggaggttcac gatatgttgt
      301 acgataaatc ccttgagagg gataactgtg gtttcggcct gatcgcccac atagaaggcg
      361 aacctagcca caaggtagtg cgtactgcaa tacacgcact ggcccgcatg cagcaccgtg
      421 gcgcgattct cgccgatggt aaaaccggcg acggttgcgg cttgctgtta caaaaaccgg
      481 atcgcttttt tcgcatcgtt gcgcaggagc gcggctggcg tttagcaaaa aactacgccg
      541 tcgggatgct attcctgaat aaagatcctg aactcgccgc tgccgcacgc cgcatcgttg
      601 aagaagaact gcaacgcgaa accttgtcga ttgtgggctg gcgtgatgtc cccactaacg
      661 aaggcgtgct gggtgaaatc gccctctcct ctctgccacg cattgagcaa atttttgtga
      721 acgccccggc aggctggcgt ccgcgcgata tggagcgccg tctgtttatc gcccgccgcc
      781 gcattgaaaa gcgtctcgaa gccgacaaag acttctacgt ctgtagcctg tcgaatctgg
      841 tgaacatcta taaaggtctg tgtatgccgg cggatctgcc gcgcttttat ctggatcttg
      901 cggacctgcg tctggaatcg gccatctgcc tgttccacca gcgcttctcc actaacaccg
      961 taccgcgctg gccgctggcg cagccgttcc gctacctggc gcataacggt gaaatcaaca
     1021 ccatcaccgg taaccgccaa tgggcgcgtg cgcgtaccta taaattccag acgccgctta
     1081 tccctgatct gcacgacgcc gcaccgttcg tcaacgaaac cggctctgac tccagttcga
     1141 tggataacat gctggaactg ctgctggcag gcgggatgga tatcatccgc gccatgcgtc
     1201 tattagtacc acccgcctgg cagaacaacc cggatatgga cccggaactg cgtgccttct
     1261 ttgactttaa ctccatgcat atggagccgt gggatggccc ggcgggcatc gtgatgtccg
     1321 atggtcgctt cgctgcctgt aacctcgacc gtaacggtct gcgtccggca cgctacgtca
     1381 ttaccaaaga caagctcatc acctgcgcct ctgaagtcgg tatctgggat tatcagcctg
     1441 acgaagtggt cgaaaaaggc cgcgtcgggc caggcgaact gatggtgatc gacacccgca
     1501 gtgggcgtat tctgcactcg gcagaaaccg atgacgatct gaaaagccgc catccatata
     1561 aagagtggat ggagaaaaac gtccgccgac tggtaccgtt tgaagatctg cccgatgaag
     1621 aagtgggtag ccgcgaactg gacgacgaca cgcttgccag ctaccagaaa cagtttaact
     1681 acagcgcgga agagctggac tccgtaattc gcgtactggg cgaaaacggt caggaagcgg
     1741 tcggttcgat gggcgatgat accccattcg ccgtgctctc cagtcagccg cgcattattt
     1801 acgactactt ccgccagcag tttgcccagg tgactaaccc gccaatcgac ccgctgcgtg
     1861 aagcgcatgt tatgtcgctc gccaccagta tcggtcgtga aatgaacgtc ttttgcgaag
     1921 cagagggcca ggcgcaccgt ttaagcttta aatcgccgat tctgctctac tccgatttca
     1981 aacagctcac gacgatgaaa gaggagcact accgcgcaga tacgctggat atcacctttg
     2041 acgtcactaa aaccacgctc gaagcgacag tcaaagagct gtgcgacaaa gccgaaaaaa
     2101 tggtacgtag cggcaccgtg ctgctggtgc tctccgaccg gaatatcgct aaagatcgcc
     2161 tgccggttcc agccccgatg gcggttggcg cgatccagac ccgtctggtc gatcaaagcc
     2221 tgcgttgcga tgccaacatc atcgtcgaaa ccgccagcgc ccgcgatccg caccacttcg
     2281 ccgtgttgct gggcttcggc gcgacggcta tttatccata ccttgcctat gaaacgctgg
     2341 gccgcctggt agacacccat gcgattgcca aagattatcg taccgtgatg ctcaactacc
     2401 gtaacggcat caacaaaggc ttgtacaaaa tcatgtccaa aatgggcatc tccaccatcg
     2461 cctcttaccg ctgctcgaaa ctgtttgaag cggtcggtct gcacgatgat gtagtgggcc
     2521 tgtgcttcca gggggcggtc agccgcattg gtggggcgag ctttgaagac ttccagcagg
     2581 atctgctgaa cctgtcgaaa cgtgcctggc tggcgcgtaa gcccatcagc cagggcggcc
     2641 tgctgaaata cgtccacggc ggcgaatacc acgcttacaa cccggacgtg gtgcgcacgc
     2701 tgcaacaggc ggtacaaagc ggcgagtaca gcgactatca ggaatacgcg aagctggtta
     2761 atgagcgtcc ggcaaccacg ctgcgcgatc tgctggcaat tacgccgggt gaaaacgcgg
     2821 tcaacattgc tgatgttgaa ccggcaagcg aactgtttaa acgctttgat accgccgcga
     2881 tgtctatcgg cgcgttaagc ccggaagccc acgaggcgct ggcggaagcg atgaatagca
     2941 tcggcggtaa ctcgaactcc ggtgaaggcg gcgaagaccc ggcgcgctat ggcactaaca
     3001 aagtgtcgcg catcaagcag gtggcttctg gtcgcttcgg cgttactccg gcgtatctgg
     3061 tcaatgccga cgtcattcag attaaagtcg cccagggcgc gaagccgggc gaaggcggtc
     3121 agttgccggg cgacaaagtc actccttaca tcgccaaact gcgctattcg gtgcccggag
     3181 tgacgctgat ctccccgccg ccgcaccacg atatctactc tatcgaggac ttagcgcagc
     3241 tcattttcga cctcaagcag gttaacccga aagcgatgat ctccgtgaag ctggtttccg
     3301 aaccgggcgt aggcaccatc gcgactggcg tggcaaaagc ttatgccgat ttaatcacca
     3361 tcgcaggcta tgacggtggc accggcgcaa gcccgctgtc gtcagtgaaa tacgcaggtt
     3421 gcccgtggga gctggggctt gttgaaaccc agcaggcgct ggttgccaat ggtctgcgtc
     3481 ataagatccg tttgcaggtc gatggcggcc tgaaaaccgg tgttgatatc atcaaagcgg
     3541 cgattctcgg cgcagaaagc ttcggcttcg gcactggccc gatggtggcg ctcggctgta
     3601 aatatctacg tatttgccat ctgaacaact gcgcaacggg tgtagcaact caggatgaca
     3661 aactgcgtaa gaaccactat cacggcctgc cgttcaaggt taccaattac tttgaattta
     3721 ttgcccgtga aacccgcgag ctgatggcac agctgggcgt aacgcgtctg gtggatctga
     3781 ttggtcgcac cgacctgctg aaagaactgg acggtttcac cgccaaacag caaaaactgg
     3841 cgctgtcgaa gctgctggag accgccgaac cgcatccagg taaggcactc tactgcaccg
     3901 aaaacaaccc accgtttgat aacggcctgc tgaacgcgca gttgctgcaa caggcgaaac
     3961 cgtttgtcga tgaacgccag agcaaaacct tctggttcga tattcgcaac accgaccgtt
     4021 ctgtcggcgc gtcgctttca ggctatatcg cccagacgca cggcgatcag gggctggcag
     4081 ccgatcctat caaagcgtac ttcaacggca ccgcaggcca gagtttcggt gtgtggaacg
     4141 cgggcggcgt ggaactgtac ctgaccgggg atgccaacga ctatgtcggt aaaggcatgg
     4201 cgggcggctt aatcgccatt cgtcctccgg ttggttccgc cttccgcagc catgaagcaa
     4261 gtattatcgg caacacctgc ctgtatggcg cgaccggtgg tcgtctgtat gccgcaggcc
     4321 gcgcgggtga acgtttcggc gtgcggaact ccggtgctat caccgtggta gaaggcattg
     4381 gcgacaacgg ctgtgaatat atgacgggcg gtattgtctg tattctgggt aaaaccggcg
     4441 ttaacttcgg tgcgggcatg accggcggct tcgcttacgt tctcgatgaa agcggtgatt
     4501 tccgcaaacg cgttaacccg gaactggtcg aggtcttaag cgttgacgat ctggcgatcc
     4561 atgaagagca tctgcgcggt cttatcaccg agcatgttca gcatactggc tctcagcgcg
     4621 gtgaagagat tctggcgaac tggtcaacct tcgccactaa atttgcgctg gttaaaccga
     4681 agtccagtga tgtaaaagca ctgctgggtc atcgtagtcg tagcgcagca gagttgcgcg
     4741 tgcaggcgca gtaaggggta gcaacaatga gtcagaatgt ttatcaattt atcgacctgc
     4801 agcgcgttga tccgccaaag aaaccgctga agatccgcaa aattgagttt gttgaaattt
     4861 acgagccgtt ttccgaaggc caggccaaag cgcaggctga ccgctgcctg tcgtgcggca
     4921 acccatactg cgagtggaaa tgtccggtac acaactacat cccgaactgg ctgaagctcg
     4981 ccaacgaggg gcgtattttt gaagcggcgg aactgtcgca ccagaccaac cccctgccgg
     5041 aagtttgcgg acgagtctgc ccgcaagacc gtctgtgcga aggttcctgc actctgaacg
     5101 atgagtttgg cgcggtgacc atcggcaaca ttgagcgtta tatcaatgat aaagcgttcg
     5161 aaatgggctg gcgtccggat atgtccggcg tgaagcaaac cggtaaaaaa gtggcgatta
     5221 tcggcgcagg cccggcaggt ctggcgtgtg cggatgtcct gacgcgtaac ggcgtaaaag
     5281 ccgttgtctt cgaccgtcat ccagaaattg gcgggctgct gaccttcggt attccggcct
     5341 tcaagctgga aaaagaggta atgacgcgtc gccgtgaaat cttcaccggc atgggtattg
     5401 aattcaaact caataccgaa gtgggccgcg acgtgcagct ggacgatctg ctgagtgatt
     5461 acgatgccgt cttccttggc gtcgggactt atcagtcaat gcgcggcggg ctggaaaacg
     5521 aagacgccga tggcgtgtac gcagcgctgc cgttccttat cgccaacacc aaacagttaa
     5581 tgggctttgg tgaaacccgc gacgaaccgt tcgtcagcat ggaaggcaaa cgcgtggtgg
     5641 tccttggcgg tggcgacact gcgatggact gcgtgcgtac gtccgtgcgc cagggagcga
     5701 agcacgttac ctgtgcctat cgtcgtgatg aagagaacat gccgggttcc cgccgcgaag
     5761 tgaaaaacgc gcgggaagaa ggcgtagagt tcaaattcaa cgtccagcca ctggggattg
     5821 aagtgaacgg taacggtaaa gtcagcggcg taaaaatggt acgtaccgaa atgggcgaac
     5881 cggatgccaa aggccgtcgc cgcgcggaga tcgttgcagg ttccgaacat atcgttccgg
     5941 cagatgcagt gatcatggcg tttggtttcc gtccacacaa catggaatgg ctggcaaaac
     6001 acagcgtcga gctggattca caaggccgca tcatcgcccc ggaaggcagc gacaacgcct
     6061 tccagaccag caacccgaaa atctttgctg gcggcgatat cgtccgtggt tccgatctgg
     6121 tggtaaccgc tattgccgaa ggtcgtaagg cggcagacgg tattatgaac tggctggaag
     6181 tttaagctct gtcccagcgc cgcctcctca gaaaagagtc ggcgctgaaa ttctcctttc
     6241 tttctggaaa gcgcctcgca taatctgaag aaaaccaatg taatacgctc atctggattg
     6301 ttaccggtag atgcccggcg tatagctgat tccattattc aacgcagcgc catataaagg
     6361 gacagataat ggaatcgctc tcagagagaa cctcaacagg ctaccagcaa atccacgacg
     6421 gtattattca tctggtcgat agcgcccgga cggaaacggt acgtagcgtt aacgcgctga
     6481 tgaccgcgac atactgggaa attggccgac gaattgtcga atttgaacaa ggtggcgagg
     6541 ccagggctgc gtatggtgcg cagctaatca agcgactatc aaaggattta agtctaaggt
     6601 ataagcgtgg gttctctgca aaaaacttac gccaaatgag gcttttttac ctcttttttc
     6661 aacatgttga aattcgccag acagtgtctg gcgaattaac acctgccctg gtccacctac
     6721 gtccgtttac tttccgttaa aaacgctgac gcccgcagct tttatgaaaa agagacgctc
     6781 cgctgtggct ggtctgttcg ccagctagaa cggcaaattg cgacccaatt ttatgagcga
     6841 acactactgt cacatgacaa atcagccatg ctgcaacaac acgctcctac cgagacgcat
     6901 attcttccgc aacaggcgat acgcgatccc tttgtgctcg aatttctgga attgaaagat
     6961 gaatattcag aatctgattt cgaggaggcg ctgatcaacc acctgatgga tttcatgctg
     7021 gaacttgggg atgattttgc ctttgttggt cggcagcgaa ggttacgcat tgatgacaac
     7081 tggtttcgtg tcgatctgct gtttttccac cgccgtttac gctgcctact aatcgtcgat
     7141 ctaaaagtgg gcaaattcag ctatagcgat gccggacaga tgaatatgta tctcaactac
     7201 gccaaagagc actggacgct accggatgaa aatccgccca tcggtctggt tctctgtgca
     7261 gagaaaggag ccggagaagc acattatgct ctggcaggtt tgcctaacac cgttctggca
     7321 agcgaatata agatgcaact acctgatgag aaacgactcg cagatgaact cgttcgaaca
     7381 caggcggtgc tagaggaagg ctatagacgc cgttaatttc agcggtttcg aattctgcag
     7441 acagtgtctg cagaatcttt aaaacgaact aagttttaag ccattaaatc agccttaacc
     7501 ttcaccacaa cctttttaat ctctccaggc tcgccgacaa cgcatcctgg tttatgcggt
     7561 tcacctggca taaacacggc gaacattccc ggttttaaga tgatggcttg ctcgttatca
     7621 atggtgctgc aaagctgata atcatcctca tggtggaact cttcacactg gcgcgcagtg
     7681 cctgccatgc caaacagaat ccgttcctca ccgtttaata acagttggat atcaatgtat
     7741 tgctcgtgca attccgcttt tttctcgacg ggcgattgag tgttaaacgt catgacattc
     7801 ataaagatat tgtcgccctg taattcgtaa cgacccggcg ctttttcttg cggtctggca
     7861 gctaatgcca gcgttaacgc gtcctgtaac gcaggatgta acccagcaga cggtaatgac
     7921 tgtacttcac ccatcatcat aatttttctc cctgggccaa cagcgcagcc ccaagtaaac
     7981 ctgcatcgtg gcggtagtgc gccgccagta aatcaacatg aaatgccgct ggctcctgcg
     8041 ccagatacgt ttccaccagc gccagatacc cttctgccag cccaacgctg ccaccgacca
     8101 ccacgcactg gcaatcagtt gtggctttaa tatcagcgat cagccttgca agcgtacgtg
     8161 cggagcggtg aatcagctgc tgcgcctgct cgtcgccctg cccggcgcgt gtgaaaatag
     8221 ttctcgcatc cgcgcccgcc agttcaccct gcgctgccgc cgcaatgccg cgaccagaag
     8281 caatcgcctc cacgcaaccc gtgcgtccac agccacagac cgggccgtgt ggatcggcaa
     8341 gcgtatgccc gatgtgcccc gccagaccgc cagggccggt aagcagtttg ccgccgctca
     8401 ctacaccgcc gccgacgcca gtggaaacgg tgataaagac catatcggtt atatcgcctt
     8461 ccagcgcctg atactctgcc catgctgcgg cctgcgcgtc gttaatggca atggtcggca
     8521 aattggtaag ttgttccagc gttttgacta acggaaagtg tagcaatcca ccgagattat
     8581 gcggattcag cgccagcaaa ctgccgtcgc gaataattcc ggttgaagcg atggcaaccc
     8641 gctgcgcatg agcttgcaac ggagagacta atgcggataa ggcatcacgc aaggcttctg
     8701 gtgtctggct ggctggcgta ggaagttcac gacgatcgcg gatctgcccg tcagcgccaa
     8761 tcagcgcggc ggcaagttta gtaccgccga tatcaatcgc cagcgtggtc atagcaccgc
     8821 ctttttcatc gctgtgttgt accactgaca aatgtgttca agacgcgtaa tggcagaacc
     8881 gaccgtcacc gcccacgcgc cgtggcgcat cgcatctgcc gcctgagcag gcgtgttgta
     8941 acgcccttcg gcaatcaccc tacatccggc gtcgctcaac gttttcacca gcgccagatc
     9001 cggctcttct ggcgtttcag gcgtggtata gccagaaagc gtagtgccaa taatttcggc
     9061 tcccagcttt tggcatgcca ggccgtcttc cggcgttgag cagtcggtca tcgccagtaa
     9121 accgtgatgg tgaatacgtg ccagcagcgt ttcaacaggc accggacgcg ggcggtcggt
     9181 gccgtcaatg gcgataatgt ccgcgcccgc ctgcgccagc gcatcaacat cttcaatata
     9241 ggccgtgatg cgtaccggag aatcctccag atcgcgtttc acaattccaa taatcggcac
     9301 gctcaccacc gcacgcgtgg cttgcagatt tgccacacct tcaatgcgaa tggcaaccgc
     9361 gcccgcctgt tctgccgcta atgccatggc ggcgacgatt tcgggtttat cgagcgggct
     9421 gtccggaacc ggctggcagg agacaatcag gccaccgtta gcagcgattt tttgatccag
     9481 ttgtgcaagt aacgacatac atcttccctt agcgaaaggc ccggtacata gaccgggcaa
     9541 caggattaac ttttggtttt gactaaatcg tttttggcgc tgccaaatgg cacggcaccg
     9601 ctaaatggtt tgccgtcgat agcgtcatga gtacgcaatg cttccgggcg cagccaacgc
     9661 tgaacgcggg aaggcatatc cagaccaatc agcaggatca ccacaaatgt cagactgaac
     9721 gatagcgatg ccagcgcagt acccagatcc agacgttgag cgatcaacgc gccgatgatt
     9781 ggggccagtg cgccgcccaa tgcaccaacg ttgtaggtaa agcccaggcc cgctgcacgc
     9841 tggtcggtat cgaaataacc gccaatcagt ttcggtaaga tcccggcgat cccttgtcca
     9901 agcatttgct ggaagaacag taacagaccg agcacccaga cgtttgcgcc gccaatcgca
     9961 aataccggaa taatcaacag ctgcgaggcc aacaggctac aaacgtacgc tttgcgggtt
    10021 cccagccagt caccgaggaa gccgccgaca cagcacccca ccgcagcccc aaagccgcta
    10081 aagaacagca cattggcgac agtatgcggg ttataagcca gatcggtttt cagatacgtt
    10141 ggcagcagcg cctgaatcgg ccatgagtag aggaaagcaa acaacacgac caccatcagc
    10201 attacgcccg ttggccagcg tttacccgta ctttgcacca taaagctgat aaagattgcg
    10261 gcgcataaca gcccaagaac agcgacgatc gcggcatttt gcaggttacc ggcgaagcaa
    10321 aaccacagcg cagtagccgc cgccagtgtc attacgatat tggcaatgcg atgttcacca
    10381 cggtagagaa tatctaccat cgtgcgtact ggtgctttac ctgcatgttt ctctttccag
    10441 tcttccgctt ccgggatgtt tttacgcagc cagagagcaa agatgatagg caaaatgccg
    10501 ataaagaaca gcgcacgcca gccccagacc ggaaccacca ggctatagac ctgagcggca
    10561 acgacggccc ccacagagaa gcctgaaatc agaaaaccac tggctttgtt acgcagatgt
    10621 tttggccagc tttcaatgac ataggtggcg ctggaaccgt attcacccgc catccccatg
    10681 ccgatgacca gacgcgcgat aaacatggtg atgtagcctg gcgcaaagcc gcaggccagc
    10741 gtcccggccg agaagagaac gatgctggtg accattgcca gacgacgccc gtagcggtca
    10801 cccatagcgc cgagcatcag gccgccgaac cagcgagaga taaaggctgc ggagatcaga
    10861 cttgccgcct gcaccgtcgt cagcccgaat tcaccttgta cttcggtgag taccagggcg
    10921 attaaaacga aatcaaaacc gtcaagcaga tatcccaacc aggcagcgga aaatgcccgc
    10981 cattgtgcac gattgagatg gcgataccac gggatattct gggttgtagt actcattgtg
    11041 agtctcccgc ggtgggcgat gcccacacgc tttggtatga aaattgtagg gtacagatgc
    11101 gtttatttcc cctcacccgg taggggcgag cgaggggaaa acaactcacc cgcgctcttg
    11161 catcaactgc tgggccagcg ccttcagttc tggcagatat ttttcatcta ccggtccaaa
    11221 cggtttgcgg cacagcggca cagaaacgac atccatataa tggaggacag ttttcaggcc
    11281 gcggaatacg cccgttttga tcagtaaatc aatgacttta ttgcattcag tttgcagttt
    11341 ctgcgcggtc tggatatcgc cttctttcag cgccttaacg atcccctgat agcgccagcc
    11401 catgatgttg taggtactac cgataccacc atcagcgccc gccagcagac cagaggcgaa
    11461 gatttcgtcg taaccgttat agagcacaag atcaggatgt tcacgacgga tctgctccat
    11521 ctgatagaga tcgccagaag tctgtttcag cgcaccgacg ccaggcaacg taaccagggt
    11581 gttgatctga tccagggtca gttttacccc actcagggct ggaatgttgt acaccaccat
    11641 cggcaaacca tccgctgaat caataattgc ccgatagtga tcgcagtgtt cttcaaagct
    11701 gaaaggatag tagaacggcg tgacggcgga gacggcatcg aagccataac gtttagccga
    11761 tgccgcaagt tgttggcttt cggcggtgct gacgcaaccg acgtgggcga tgagtttaat
    11821 cttacctttc gcctcttcgg cgacgatttc cagtacctgt tcacgctcgg aaaggctttg
    11881 tacaaaggcc tcgccggtcg aaccacccac gtataaaccg tcgatgccct gctgaatatt
    11941 gaactgaacc aggcgacgca gactcgcttt atccagtgct tgttgttgat caaaaggagt
    12001 caggagtgca gccattacgc cacgtaaatt cgttgccata aatacctctg aagtgatgct
    12061 tgtctgataa acgatatacc tttatacctg ttataccaga tcaattaagc aacaccccat
    12121 acagaaagct tataatgcga tctgcttcac taaagtggca ttatttcttt ttgttggtgg
    12181 tctgaccgaa agcgtgccag gtagcagaga cgctgttgag atgcgattgc aacgcacgat
    12241 cggcttcgtc aggatcatga cggcggatcg catcaacgat cgcaatatgc tgttgataac
    12301 taacgttgtt atgttcgtgc agtgcctgat cggtaaccgt tgggcgtgcg gcaataagcc
    12361 agtcgagcag ggcaacgtgg atcgccatga agattgggtt accggggatc tccgccagca
    12421 cgcggtggaa atcaacgtct gaacgaatga atgccgcgtt gttatccagc gactgactgt
    12481 taatttccag tgcttttgcc agcaaatcga tttgctcatc ggtggcatgt tcagccgcat
    12541 agcgcaccag gctggattca aagaacagac gtaattgttc gaaatgggca atcccaccag
    12601 gatgagaaag gaaatctttc gccatgccgg aaagctcgcc gatgatggtg tccgcagaag
    12661 gacgtgagac gcgggcacgt tcgccgttgt tgatttgcac cagacctttg cgttttaacg
    12721 ctgccagcgc ttcgcgcaca gaaggacgcc cgacgttaaa gaacgccatc agttcgcgtt
    12781 cagacggtaa ttgttcacct tcgccaaatt cacgacggcg gatcatctgt tccagctctt
    12841 cttccaccat ttcggagagt tttttacgcg ccagcgggcg gctacgcaag ttgcgaccaa
    12901 ttgcaggtga agaatcttcg gtttgcgaat caaatgcgtt cataaggccc attctgtaag
    12961 gtcagtgtga ttaacatcat cagtgacatc ctatcacagg attgaaagta ggggaaaatg
    13021 gcaggttttt ctctttgtgc ctcatca
//
LOCUS       DAZGLJ010000126        13042 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000126 DAZGLJ010000000
VERSION     DAZGLJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.126"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            242..823
                     /gene="mdaB"
                     /locus_tag="ACHK49_003161"
     CDS             242..823
                     /gene="mdaB"
                     /locus_tag="ACHK49_003161"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO9263770.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            854..1168
                     /locus_tag="ACHK49_003162"
     CDS             854..1168
                     /locus_tag="ACHK49_003162"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO9263771.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1216..3108)
                     /gene="parE"
                     /locus_tag="ACHK49_003163"
     CDS             complement(1216..3108)
                     /gene="parE"
                     /locus_tag="ACHK49_003163"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO9263772.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3137..3718)
                     /gene="yqiA"
                     /locus_tag="ACHK49_003164"
     CDS             complement(3137..3718)
                     /gene="yqiA"
                     /locus_tag="ACHK49_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO9263773.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3718..4545)
                     /gene="cpdA"
                     /locus_tag="ACHK49_003165"
     CDS             complement(3718..4545)
                     /gene="cpdA"
                     /locus_tag="ACHK49_003165"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO9263774.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYRHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4570..4992)
                     /locus_tag="ACHK49_003166"
     CDS             complement(4570..4992)
                     /locus_tag="ACHK49_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO9263775.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(4993..5622)
                     /gene="nudF"
                     /locus_tag="ACHK49_003167"
     CDS             complement(4993..5622)
                     /gene="nudF"
                     /locus_tag="ACHK49_003167"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO9263776.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5827..7308
                     /gene="tolC"
                     /locus_tag="ACHK49_003168"
     CDS             5827..7308
                     /gene="tolC"
                     /locus_tag="ACHK49_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO9263777.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7456..8127
                     /locus_tag="ACHK49_003169"
     CDS             7456..8127
                     /locus_tag="ACHK49_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO9263778.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8133..9293
                     /locus_tag="ACHK49_003170"
     CDS             8133..9293
                     /locus_tag="ACHK49_003170"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO9263779.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9331..10119)
                     /gene="ygiD"
                     /locus_tag="ACHK49_003171"
     CDS             complement(9331..10119)
                     /gene="ygiD"
                     /locus_tag="ACHK49_003171"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO9263780.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10262..11035
                     /gene="zupT"
                     /locus_tag="ACHK49_003172"
     CDS             10262..11035
                     /gene="zupT"
                     /locus_tag="ACHK49_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO9263781.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11093..11263)
                     /gene="yqiD"
                     /locus_tag="ACHK49_003173"
     CDS             complement(11093..11263)
                     /gene="yqiD"
                     /locus_tag="ACHK49_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO9263782.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11524..12177)
                     /gene="ribB"
                     /locus_tag="ACHK49_003174"
     CDS             complement(11524..12177)
                     /gene="ribB"
                     /locus_tag="ACHK49_003174"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO9263783.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12281..12429)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12551..12841
                     /gene="ubiK"
                     /locus_tag="ACHK49_003175"
     CDS             12551..12841
                     /gene="ubiK"
                     /locus_tag="ACHK49_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO9263784.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3312 a         3473 c         3178 g         3079 t
ORIGIN      
        1 gccatatttt caatcccaaa aaaaaccgaa ccaaacgtgt aaattattcc ggtgcgagta
       61 agaattatga ggaatggcta tcagtattgt cattttcaga aaatatttat cctgcatcgg
      121 tgagtcagag caagatcaga cttttgctaa attcgcaaaa gactttgcac attttgctaa
      181 tttcaccgta ccgctctgtg acgtactata gtcggcaaac gtctcacctt gaggttaaaa
      241 aatgagcaac atcctgatta tcaacggcgc gaaaaaattc gcccactcca atggtcaact
      301 gaacgacacc ctgaccgaag tcgcggatgg cacactgcgc gacctcgggc atgatgtccg
      361 catcgttcgc gccgacagcg actacgatgt caaagcggaa gtacaaaact ttctctgggc
      421 tgatgtggtg atctggcaga tgccaggctg gtggatgggc gcgccgtgga cagtgaaaaa
      481 atatattgat gatgtattca ccgaaggtca tggaacgctg tatgccagcg atggccgtac
      541 ccgcaaagat ccgtcaaaaa aatacggttc cggcggcctg gtacagggca aaaaatatat
      601 gctttctctg acctggaacg caccaatgga agccttcacc gaaaaagatc aattcttcca
      661 cggcgttggc gtcgacggtg tgtatctgcc gttccataaa gcaaaccaat tccttggcat
      721 ggaaccgctg ccgacattta tcgctaatga cgtgataaaa atgcctgatg ttccccgcta
      781 tactgaagaa tatcgcaagc atcttgtgga aatttttggt taactagagc tcaggcttta
      841 gaaggagtta accatgctta ccgtaatcgc agaaatccgt actcgtcctg gtcaacatca
      901 ccgtcaggcg gtattggatc agtttgctaa aatcgttcca accgtactga aagaagaagg
      961 ttgccacggc tatgcgccaa tggtggattg cgctgctggc gtgagtttcc agtctatggc
     1021 accggattct atcgtgatga ttgagcagtg ggaaagcatc gcgcatcttg aagcgcatct
     1081 gcaaaccccg cacatgaagg cgtacagcga agccgtaaaa ggcgacgtgc tggagatgaa
     1141 tatccgcatc ctgcaacctg ggatttaaac ctgccttgtt tgcccggcca tcctgaccgg
     1201 gcaatgttct ttcctttaaa cctcaatctc cgccatgtcg cctttctctt gcaaccagtt
     1261 gcggcgatct tccgagcgtt tcttcgccag cagcatatcc atcatcgcgt cagtacgctg
     1321 atcgtcttca tcatcgatag tcaactgcac cagacggcga gtgttcggat caagcgtggt
     1381 ttcacgcaat tgcatcgggt tcatttcccc cagaccttta aaacgctgga cgttcggctt
     1441 gcctttcttg cgttttaatt gctcaagtac gccctctttc tcttcttccg tcagcgcgta
     1501 ataaacctct ttcccgagat caatacggta gagcggtggc agtgcgacgt aaacgtgacc
     1561 gtgtttcacc aacgcgcgga agtgttttac gaacaaagcg cagagcagcg tggcaatgtg
     1621 cagaccatca gagtccgcat ccgcgaggat acagattttg ccataacgaa gctggctcag
     1681 atcgtcgctg tcaggatcga taccgatcgc taccgaaata tcatgcactt cctgcgaggc
     1741 cagcacttcg tcggaagaaa cttcccaggt gttaaggatc ttacctttca gtggcatgat
     1801 cgcctgatat tcgcgatcgc gcgcctgctt ggcagatccg cctgcggagt caccttccac
     1861 aaggaacagt tcggtacggt taagatcctg cgcggtacag tcagccagtt tgccaggcag
     1921 tgctgggccg ctggtcagct ttttgcgcac cactttttta gccgcacgca tacggcgctg
     1981 ggcgctggaa atcgccatct ccgccagcag ctccgccgcc tgaacgttct ggttcagcca
     2041 caggataaag gcatctttca ccacgcctga aacgaatgcc gcgcattgac gcgaagagag
     2101 acgctctttg gtctgcccgg caaactgcgg atcctgcatt ttcaccgaca gaacataggc
     2161 gcagcgatcc cagatatctt ccgccgacag cttcacgccg cgcggcagaa tgttgcggta
     2221 ttcacagaac tcacgcatcg cgtccaacag gccctgacgc agaccattaa catgggtacc
     2281 gccctgcatc gttgggataa ggttgacgta gctttcggtc agcagttcac cgccttccgg
     2341 cagccacagt agcgcccagt ccacagcttc agtatcgcca gcgaaattac cgataaacgg
     2401 tttttctggc agcgtcggta aaccgtttac cgcttccgcc aggtaatcat tcagaccgtc
     2461 ctgatagcac cagcgctgtt cggtgttgtt gatctcatct ttaaaggtaa tctcaacgcc
     2521 ggggcacagt actgctttgg ccttcagcac atgcgtcagg cgtgaaacag aaaaacgcgg
     2581 gctgtcaaag aaggtttcat ccggccagaa gtgcacgctg gtaccggtat tgcgtttacc
     2641 gcaagtgccg acaacctgta aatcctgcac cttttcgcca ttttcaaagg cgatgttata
     2701 aacctgacca tcgcggcgca cgttaacttc tacgcgcttc gacagggcgt taaccaccga
     2761 aatccccacg ccatgcaggc cgccagagaa ctggtaattt ttgttagaga atttaccgcc
     2821 tgcatgcaga cggcaaagaa tcagttcaac cgccggtaca ccctcttccg ggtgaatatc
     2881 caccggcatc ccgcgcccat cgtcaataac ttctaacgac tggtcagcat gtaagattac
     2941 atccacgcgt tttgcgtgac ccgccagtgc ttcatcgaca ctgttatcaa taacttcttg
     3001 ccccaaatgg ttagggcgag tggtatcggt atacatcccc ggacggcggc gaaccggctc
     3061 aagcccggtg agtacctcaa tggcatcagc gttataagtt tgcgtcatgg tttaagttag
     3121 caattcgagt tgatcgtcag agatggtgca gaccaagaaa atcgacgatc gggttgaaat
     3181 aatcttcgaa gcccgtgaat gcgtggttgc cgccttctat gacagtctgg cggcaggaag
     3241 cgtagtacgc caccgcctgg cggtaatcca gcacttcatc tcccgtctgt tgcagcagcc
     3301 agatcaaatc cggcgcttcc agcgggtcaa tctgcatgac tttgagatcg taaatatggc
     3361 gtgactctag cacatattgc tgcccggtgt aggggttctc gttctgaccg agatagtccg
     3421 tcagcagttc aaacgggcgc accgccgggt ttaccaccac tgcgggcagc ataaaacatt
     3481 gtgacaacca ggtggcgtaa tatcccccca gtgacgaacc gacaataccc agcgaatcgc
     3541 cgccatgttc caggacaatg gattccagca gctctgccgc gtcggaagga tacggcggca
     3601 actgcggaat gatcatctca acgtcagggt gatgttccgc cagccagttt tttaacaagc
     3661 tcgcttttgc agagcgcggc gagctgttga aaccgtgtaa ataaagaagc gtagacatca
     3721 gtagccttct gaagcggtat caggttggaa acgtgtgtcc gccaggcgat gcacctcggt
     3781 ggtcagcgtg ccatcagcat gtaactcgag agtacgccag ccgggcgcga tggtgtccag
     3841 cgtaaagttg gaacagtgcg gcttaaactg cacacaggtc gacggcgttg ccagcaggcg
     3901 gcgaccgttc cagtcgagat ccagctcctg atgaatatga ccgcacagca agtatttgac
     3961 gtgcggaaac ttcgccagca cggtatccag ttcgcccgcg ttacgcaggc tgtgttgatc
     4021 aagccaacta caacccgcag gtagcggatg atgatgcagc agcagcaacg tatggcgttc
     4081 tggcgcatcg gccagtttac gttccagcca ctcaagctga aactcgctca gctcaccgtg
     4141 cggcacgcca aacacctggc tatccagcaa caggatttgc cattgctcac caataaacac
     4201 ccgcttcgcc ggggagatac ccgcatcctg taacgcgctg tacatcgcgg gctggaaatc
     4261 gtggttgcca ggcagccaga cgcagggcgc acgaaaactt gcgatgcctt cagcgaaatg
     4321 ccgataggcc gcagaggatt gatcctgcgc taaatcacct gtcgcgacaa tcaggtcgaa
     4381 ttcgtgctgg tgtggacgaa tcgcctccag caccgcctgg tagctttccc aggtatttac
     4441 ccctaacagg gcttcgtgct tttgtgcaaa caggtgagta tcggtaattt gtaaaatcct
     4501 gactctggcc tcaccagcca gaggaagggt taacaggctt tccaaatggt gtccttaggt
     4561 ttcacgacgc tagtaaaccg gaatcgccat cgctccatgt gctaaacagt atcgcaacca
     4621 gtccgctaaa aactggttaa tttgatgctt ttcgtcgcgt tgatgcaact ttttattagg
     4681 ataatcatac cgcgctttga agcgaaaaat ctgctggctt gaacacactt cagccaccat
     4741 cgcgtcatga tacagacgca ccgtcattga cggaaggctc cagtaactga tcgcgggcgc
     4801 agtctgttct attgtcacca gggtagtgta tcgggtcgat tccacaatcg tcagccgata
     4861 ttgtgcgttt gccacctgat agcttacagt ttcgccgggt gcgtcattgc gcggtaacaa
     4921 acggcgcaat tgtgaaaagt tcatctcgca caggcgcatc atttcaggaa agtcaggtgt
     4981 gtaacgcttc atttatgccc actcattttt taacgcttga tgatgcagct gcagccattg
     5041 caaagcgatg accgacgctg cgttgtcgat tttcccctct tctacccact ggtatgcctg
     5101 ttcccggctt accacatgaa cgcgaatatc ttcgttttca tcagccagac catgaatacc
     5161 gcttgcggtc gtggcgtcca cttcgcccac cataattgac gaacgctcac tggtgccccc
     5221 cgggcttgcc aggaaactta acaccggttt ggtccgtttg actatcagtc ccgcctcttc
     5281 aatcgcttcg cgacgggcaa catcttccac actttcaccc tcttctatca tcccggcaac
     5341 catctccagt agccaggggg tttcgctggt gtcgtacgcg gcaatccgaa tctgctcaat
     5401 cagcacaact tcatcacgca ctgggtcaaa gggtagcaag actgcggcgt gaccgcgctc
     5461 aaaaatttcc cgccgtacct catgactcat ttgcccgttg aatagacgat gacgaaatct
     5521 ataaagatct aatgaaaaaa agccgcgata aagtgtttct cgtgcaataa tttctacatc
     5581 gtttttgcca aatgtaacgg gcaggttgtc tggcttaagc attgttaatg tcctggcact
     5641 aatagtgaat taaatgtgaa tttcagcgac gtttgactgc cgtttgagca gtcatgtgtt
     5701 aaattgaggc acattaacgc cctatggcac gtaacgccaa ccttttgcgg tagcggattc
     5761 tgctagaatc cgcaataatt ttacagtttg atcgcgctaa atactgcttc accacaagga
     5821 atgcaaatga agaaattgct ccccattctt atcggcctga gcctttctgg gttcagttcg
     5881 ttgagccagg ccgagaacct gatgcaagtt tatcagcaag cacgccttag taacccggaa
     5941 ttgcgtaagt ctgccgccga tcgtgatgct gcctttgaaa aaattaatga agcgcgcagt
     6001 ccattactgc cacagctagg tttaggtgca gattacacct atagcaacgg ctaccgcgac
     6061 gcgaacggca tcaactctaa cgcgaccagt gcgtccctgc agttaactca atccattttt
     6121 gatatgtcga aatggcgtgc gttaacgctg caggaaaaag cagcagggat tcaggacgtc
     6181 acatatcaga ccgatcagca aaccttgatc ctcaacaccg cgaccgctta tttcaacgtg
     6241 ttgaatgcta ttgacgttct ttcctataca caggcacaaa aagaagcgat ctaccgtcaa
     6301 ttagatcaaa ccacccaacg ttttaacgtg ggcctggtag cgataaccga cgtgcagaac
     6361 gcccgcgcgc agtacgatac cgtgctggcg aacgaagtga ccgcacgcaa taaccttgat
     6421 aacgcggtag agcagctgcg ccagatcacc ggtaactact atccagaact ggcagcgctg
     6481 aatgtcgaaa actttaaaac cgacaaacca cagccggtta acgcgctgct gaaagaagcc
     6541 gaaaaacgca acctgtcgct gttacaggca cgcttgagcc aggacctggc gcgcgagcaa
     6601 attcgccagg cgcaggatgg tcacttaccg acgctggatt taacggcttc taccgggatt
     6661 tctgacacct cttatagcgg ttcgaaaacc cgtggtgccg ctggtaccca gtatgacgac
     6721 agcaatatgg gccagaacaa agtgggcctg agcttctcgc tgccgattta tcagggcgga
     6781 atggttaact cgcaggtgaa acaggcacag tacaactttg tcggtgccag cgagcaactg
     6841 gaaagcgcgc atcgtagcgt cgtgcaaacc gtacgttcct ccttcaacaa cattaatgca
     6901 tctatcagta gcattaacgc ctacaaacaa gccgtagttt ccgctcaaag ctcattagac
     6961 gcgatggaag cgggctactc ggtcggtacg cgtaccattg ttgatgtgtt ggatgcaacc
     7021 accacgctgt acaacgctaa gcaagagctg gcaaatgcgc gttataacta cctgattaat
     7081 cagctgaata ttaagtcagc cctgggtacg ttgaacgagc aggatctgct ggcactgaac
     7141 aatgcgctga gcaaaccggt ttccactaat ccggaaaacg ttgccccgca aacgccggaa
     7201 cagaatgcta ttgctgatgg ttatgcgcct gatagcccgg cacccgtcgt tcagcaaaca
     7261 tccgcacgca ctaccaccag taacggtcat aaccctttcc gtaactgatg acgacgacgg
     7321 ggcttcggcc ccgtctgaac gtaaggcaac gtaaagatac gggttatctg ccgcattctt
     7381 cccccttctc gcttcaattt cgaccagcca tcctctattc tgatgggtat ttaccactgg
     7441 tcccggaaga caaaaatgaa acggacaaaa tccatacgcc acgcatcgtt ccgcaaaaac
     7501 tggagcgcgc gccatctgac accagtcgct ctcgcggttg ccactgtttt tatgctggct
     7561 ggctgtgaaa agagtgatga aacagtgtct ctctatcaaa atgctgacga ctgttcagct
     7621 gcaaacccag gcaaaagcgc cgaatgtacc accgcgtaca acaatgcgct gaaagaagcc
     7681 gaacgtactg cgccgaaata cgccacccgt gaagactgtg ttgctgaatt tggtgaaggt
     7741 cagtgccagc aggcaccagc ccaggctggc atggcaccag aaaaccaggc gcaggcccag
     7801 caatccagcg ggagtttctg gatgccgctg atggccggtt acatgatggg gcgtctgatg
     7861 ggcggcggcg cgggatttgc acagcagccg ctgttctcct cgaaaaaccc agccagcccg
     7921 gcttacggta aatataccga cgcgacgggt aaaaactatg gcgcagccca gccaggccgc
     7981 accatgaccg taccgaagac agccatggca ccaaaaccgg cgaccaccac taccgttacc
     8041 cgtggcggtt ttggtgaatc tgttgccaaa caaagcacta tgcagcgtag cgcaactggc
     8101 acctcttctc gttcaatggg tggctgatac cgatggaaag agtcagtatt accgagcgcc
     8161 cggactggcg tgagaaagcc cacgaatacg gtttcaattt tcacaccatg tacggcgagc
     8221 cgtactggtg tgaagatgct tactacaagt tgaccctcgc ccaggttgaa aagctggaag
     8281 aagtcaccgc cgaactgcac cagatgtgcc tgaaagtggt ggaaaaagtg atcgccagcg
     8341 atgagctgat gaccaaattc cgcattccaa aacacacctg gagttttgtg cgccagtcat
     8401 ggctgacgca ccagccatcg ctttattcgc gtcttgatct ggcgtgggat ggcactggcg
     8461 aacctaaact tctggaaaat aacgccgata cgccaacgtc actatacgag gcggcgttct
     8521 ttcagtggat ctggctggaa gatcagctta acgccggtaa cttgccggag ggcagcgacc
     8581 agtttaacag tctgcaagaa aaactgatcg atcgcttcgt tgagctgcgt gaacagtatg
     8641 gcttccagtt gctgcatctc acctgctgtc gcgacacggt ggaagatcgc ggaaccattc
     8701 agtatttgca ggactgcgca acggaagctg aaattgctac tgagttcctc tacatcgatg
     8761 atatcgggtt aggtgaaaaa ggtcagttca cggatttaca ggatcaggta atttccaacc
     8821 tgttcaagct gtatccgtgg gaatttatgt tgcgtgagat gttttccacc aagctggagg
     8881 atgcaggcgt acgctggctg gaaccggcgt ggaagagcat tatctccaac aaggcacttc
     8941 taccgctact gtgggagatg ttcccgaatc acccgaacct gctgcccgct tattttgcgg
     9001 aagatgatca tccgcaaatg gaaaaatatg tggttaaacc gatcttctcc cgtgaaggcg
     9061 caaacgtgtc gatcattgaa aacggcaaaa ccattgaagc agcggaaggt ccgtatggcg
     9121 aagaagggat gattgttcag caattccacc cgttaccgaa attcggcgac agctatatgc
     9181 tgattggtag ctggctggtg aacgatcaac ccgccggaat tggcattcgt gaagaccgtg
     9241 cattgatcac ccaggatatg tctcggtttt atccacatat ttttgttgag taatcaatga
     9301 tgccggatgg cattcgccat ccggtaattg ttaacctatc tgcaccgaca gcatactcag
     9361 gctgcccatt tctataccat cgaccggaat ggtaattggc tcctgcccat cccacgcacc
     9421 taagacatac aacaacggca gatagtgctc cggcgttggg ttcgataacg cgccaccttc
     9481 atggtcgagg taattcacca gaggatgctg ctctaccgcc ccttgccacg tcagattcgc
     9541 tttcacatac tcattaaacg acatcgccca aggatatggt gaactatcac cgtgccactt
     9601 cactgtacgc aggttatgca ccacgttgcc actggcgacc aacattattc cttcatctcg
     9661 cagcgctgcc agtttgcgcc ccatttcgaa atgccaggcg gcaggtttgc tactgtcgat
     9721 actcaactgc accatcggga tatcggcgtc aggatacatc ttaatcagca cgccccagga
     9781 accgtggtca aagccccagg cttctttatc cagcgtcacc gggaccggtg ctaacagctc
     9841 aaccagatgc tgtgccagca caggcgaacc cggagcagga taatgcgtat cgtacagcgc
     9901 ctgcgggaag ccaccaaagt catgaatcgt gggcggcgtc tccatcgcgg tcactcctgt
     9961 tccacgggta aaccagtgag ccgaaaccac cacaatcgct tgcgggcgtg gcaatgtcat
    10021 ccccaacttc tgccagctgc gggtatacaa attatcttcc agcacgttca tcggactacc
    10081 gtgacctaaa aacaatgctg gcatacgtgt tgaagacatg atgatatcct taactaaagg
    10141 tgtcattttg atatcctcac aatacgcttg ttcggcggag taagaacccg gataacaatg
    10201 atgatgatca tcagttattt tgacgatctg cctgaaggtg aagatttata aggagttgtc
    10261 gatgtcagta cctctcattc tgaccatact ggcgggggca gccacgttta ttggcgcgtt
    10321 tctcggcgtt ctcgggcaaa aaccctcgaa ccgcttactg gcgttttcgc taggatttgc
    10381 ggcggggatc atgttgctca tctcattaat ggaaatgctt cctgccgcac tagcagctga
    10441 aggaatgtcg cctgtgttgg gttatggaat gtttatcttc ggtctgcttg gctattttgg
    10501 cctggaccgc atgttgccac atgcgcatcc gcaggattta atgcaaaaat cggtgcagcc
    10561 gttgccaaaa tcgatcaagc gcacagccat tctgctcact ctcggcatca gtctgcataa
    10621 cttcccggaa gggattgcca cctttgtcac ggcgagcagc aacctggagc tgggatttgg
    10681 catcgcactg gccgtcgcgt tgcacaatat tcctgaaggt ctggcggttg caggcccggt
    10741 ttatgcagca acgggttcta aacgcacggc aattctgtgg gcggggattt ctggattggc
    10801 agaaattctt ggtggtgtgc tggcgtggtt aatcctcggt agcatgattt cccccgtggt
    10861 catggcggca atcatggcgg cggttgcagg aattatggtg gcgctctcgg ttgatgaatt
    10921 aatgccgctc gccaaagaga ttgaccctaa taataacccc agctatggcg tactatgtgg
    10981 aatgtcagtg atgggattca gtttagtgct gctacaaacg gcgggaattg gttaaaagca
    11041 acatcagaag ttggccagcg catggacaac ttctgctagc ttcactaaag ccttattcta
    11101 ccttttcggc tgtctcttct ctcgtactgt ttaagtattt gttccgcgct tcaagcagtg
    11161 cgtctctgtc ctggcgaaac gcccgacaat aacgtttcaa atgcaggaaa taacccacca
    11221 caacaagagc aatcaataca atccagtacc aggcgataaa catcctttta cccttaatat
    11281 ttaacaatac taaacattta gcgcataaat ttcacatatc ctttttcgga tatattcata
    11341 tggtcgcata ataacggaac aattatatga ttttgatcat aatgaaaaag aatgcctgat
    11401 attaatgaaa ttttctcata gaaagagaag cggaattaac atcttgcata gcagggcaaa
    11461 ataaaaaccg aagccagcta caacttcggt ttcttgatta aggcagtaat taagcagcgg
    11521 ttttcagctg gctttacgct catgtgcctg acggtatgcc accaggtctt caatagtcac
    11581 gagcgccata ttgtgtttat tggcaaactc aatgcactct ggtgcacgcg ccatcgtgcc
    11641 atcgtcatta gtcaactcac acagcacacc agccggttta aagcctgcca gcgtcatcaa
    11701 atcaatagtc gcttcagtat gaccgccacg cgtcagtaca ccacctgcct gcgcgcgaag
    11761 tgggaaaacg tggcccgggc gattcagatc agaaggtttt gcgccatcgg caatcgctgc
    11821 gcgaacggtc gtaattcgat cagcggcaga aacaccggta gtcacacctt cagccgcttc
    11881 aatggtcacg gtaaaaccgg tgccataggc gctggtgtta ttttctacca tcattggcag
    11941 atcaagttgt ttacggcgat cttcagtgat gcacaggcaa acaataccgc taccgtggcg
    12001 aatggtcagc gccatctgct caacggtcat ggtttctgcc gggaagatca tatcgccttc
    12061 gttttcacgg tcttcatcat caagcaccat tacaccgcgt ccttcacgca gcgcagccag
    12121 tgcattttca acacgttcga aaggcgtacc aaaagaggaa agtagcgtct gattcatggt
    12181 aaaaaaacct cactaaaatt atggttacca gaatcagggc agtcttagga gtggcggcat
    12241 atagccaaaa taacgtgagc gggtccatgc ccgacagaat cgttactctc tcccatccgg
    12301 actctaaccg tcggccccgg aattacaccg gatctgctgt cctttgagtt cgcacccaaa
    12361 gcgctcgcgg gctttcaact gagttgattt accgccggtg gggaatttcg ccccgccctg
    12421 agaataagcg gattcactat aacgctaatg attagcggca gcaacgcata gcttcacata
    12481 attctggttt atgacttacc cttatcgcac tacaatggca ctcaacacct atcagtacag
    12541 ggaaactaca atgattgacc cgaaaaaaat tgagcaaatc gctcgccagg ttcacgaatc
    12601 aatgcctaaa ggaatcaggg agttcgggga agatgtggag aaaaaaatcc gccaaaccct
    12661 acaagctcag ctgacgcgcc tcgaccttgt aagccgtgaa gagttcgacg tccaaacgca
    12721 agtcctgtta cggactcgtg agaaactggc gctgcttgaa caaagaatga gcgaactgga
    12781 aaatcgcagt actgagatta aaaagcagcc agatccagag actctccccc caacgctgta
    12841 aacatttgtc tttaatgcac tgtaaattat gaattaatcc tctaatttac agtgctttct
    12901 attaccttat cgcttaattc cattcagaat atcatcacaa aaatgaatga gtgcaattga
    12961 aaatataatt gttatgtatt ttttaagttt tacttaaccc atatttagat aaactaattt
    13021 cataaaaccc gaaaagtaaa at
//
LOCUS       DAZGLJ010000127        13010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000127 DAZGLJ010000000
VERSION     DAZGLJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.127"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(640..1731)
                     /gene="ychF"
                     /locus_tag="ACHK49_003176"
     CDS             complement(640..1731)
                     /gene="ychF"
                     /locus_tag="ACHK49_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO9263785.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1848..2432)
                     /gene="pth"
                     /locus_tag="ACHK49_003177"
     CDS             complement(1848..2432)
                     /gene="pth"
                     /locus_tag="ACHK49_003177"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO9263786.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2710..2988
                     /gene="ychH"
                     /locus_tag="ACHK49_003178"
     CDS             2710..2988
                     /gene="ychH"
                     /locus_tag="ACHK49_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO9263787.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3043..4722)
                     /gene="dauA"
                     /locus_tag="ACHK49_003179"
     CDS             complement(3043..4722)
                     /gene="dauA"
                     /locus_tag="ACHK49_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO9263788.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4847..5794)
                     /gene="prs"
                     /locus_tag="ACHK49_003180"
     CDS             complement(4847..5794)
                     /gene="prs"
                     /locus_tag="ACHK49_003180"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO9263789.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5945..6796)
                     /gene="ispE"
                     /locus_tag="ACHK49_003181"
     CDS             complement(5945..6796)
                     /gene="ispE"
                     /locus_tag="ACHK49_003181"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO9263790.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHASFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6796..7419)
                     /gene="lolB"
                     /locus_tag="ACHK49_003182"
     CDS             complement(6796..7419)
                     /gene="lolB"
                     /locus_tag="ACHK49_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO9263791.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7633..8889
                     /gene="hemA"
                     /locus_tag="ACHK49_003183"
     CDS             7633..8889
                     /gene="hemA"
                     /locus_tag="ACHK49_003183"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO9263792.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8931..10013
                     /gene="prfA"
                     /locus_tag="ACHK49_003184"
     CDS             8931..10013
                     /gene="prfA"
                     /locus_tag="ACHK49_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO9263793.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            10013..10846
                     /gene="prmC"
                     /locus_tag="ACHK49_003185"
     CDS             10013..10846
                     /gene="prmC"
                     /locus_tag="ACHK49_003185"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO9263794.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10843..11235
                     /gene="sirB2"
                     /locus_tag="ACHK49_003186"
     CDS             10843..11235
                     /gene="sirB2"
                     /locus_tag="ACHK49_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO9263795.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11239..12048
                     /gene="sirB1"
                     /locus_tag="ACHK49_003187"
     CDS             11239..12048
                     /gene="sirB1"
                     /locus_tag="ACHK49_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO9263796.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYVQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            12084..12938
                     /gene="kdsA"
                     /locus_tag="ACHK49_003188"
     CDS             12084..12938
                     /gene="kdsA"
                     /locus_tag="ACHK49_003188"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO9263797.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3270 a         3253 c         3353 g         3134 t
ORIGIN      
        1 tatatcgagt tattccctaa gttgtcgttg cttattccaa ttcgttacta taaatatggc
       61 aaaaatatta caacaaacag cggttaaaaa atttggtgaa gattttttgt aataatccta
      121 attagttatg ggaatttcgc ttgatgtatc tgcataacaa aaacaatatg cgccattttt
      181 gcattactaa taagaaaaag caaaaaacat cgatattcat ttttggtcaa tgggttttct
      241 ggcctatcgt tgttattgtg ctactggtta tcattttcat cttgccagga tcgcacgtaa
      301 tcacgttatt cggggacttt atctgcggga tattaccctt caggtaagtc cttttctcgc
      361 cagcatattg gagcgtgatc aatattgatc agctgtgaag ggggtgatgt gtagtggacc
      421 aaaacttacg ttatatcact actaatgaga tatccgtcat ctgacactga tgtctttaat
      481 tattctcgtg gttcactgta ggcaataagc acaaaagtgt aggatgttac aagaatgatt
      541 aggactcggt gaaatgaaaa atccacgcaa ttgcgtggat tatatatact tttgcgctct
      601 tcatgagatt tagcgaaacc tcatgagaca acaaattaat tagacgttga aaaggaagtt
      661 catcacatcg ccatctttaa cgatgtaatc tttaccttct gcacgcattt tgcctgcttc
      721 tttcgcgcct tgttcacctt tgtaagtgat gaaatcttca aacgagatgg tttgtgcacg
      781 gataaagcct ttttcaaaat cagtatggat tttgcccgct gcctgcggcg cggttgctcc
      841 aaccggaatg gtccatgcac gcacttcttt caccccagcg gtgaagtaag tttgcaggtt
      901 cagcagctta taaccggcac ggatcacacg gttcaggccc ggctcttcca gcccaagctc
      961 ctgcataaac tcgtcacgtt cttcgtcgtc cagttcggca atgtctgctt caacagcagc
     1021 acaaaccgga accacaacag aaccttcttt cgccgcgatt tcacgcacct ggtcaagata
     1081 tgggttgttt tcaaaaccgt cttcgttgac gttggcgatg tacattgttg gtttcagcgt
     1141 caggaagctc aggtaacgaa tagccgcttt ctcttcagcg cttaaatcca gcgcgcgcag
     1201 catacctgca ttttccaact ggggcaggca tttttccagg accgccagct cagctttcgc
     1261 gtctttatcg ccacctttgg ctttcttctg tacgcgatga atcgcacgtt cgcaggtgtc
     1321 gaggtctgcc agcgccagtt cggtgttgat aacttcaata tcgtcagccg ggttaacttt
     1381 gccggaaacg tgaatgatgt tgtcattttc aaagcagcga acaacgtgac cgatcgcttc
     1441 ggtttcacgg atgttggtca ggaactggtt acccagacct tcgcctttcg atgcgccttt
     1501 taccagaccg gcgatatcga caaattccat ggtcgtggga agcgtacgtt gcggttttac
     1561 gatttcagcc agttgatcta ggcgagggtc aggcattggt acgacgcctg tgttcggctc
     1621 aatggtgcag aatggaaagt tggccgcttc aataccggct ttggtcagcg cgttgaacag
     1681 ggtagatttc ccgacgttgg gcaaaccgac gataccgcat ttgaatccca tgatttaaat
     1741 caccttaata tcttaataat caacctgtta ttgctaacag attgcagaaa tggaaataac
     1801 tttgcctatt atacacggca ctcggcaaaa atgccgcaga caacgactta ttgcgcttta
     1861 aaggcgtgca atcggttcgt tgctttggtc aagccatctg taaaccacat ttcagtacaa
     1921 cgcgccgctt cgtcgatggc ttcatcaatt aacttctgtt cactaacagg cggtttgcct
     1981 aacacaaaac cgacaacttt atttttatcg cccggatgac cgattccgat gcgtaaacgg
     2041 tgaaagttag ggttattacc caatttactg atgatgtctt tcagtccatt gtgaccacca
     2101 tggccaccgc ccaatttaaa tttggcgacg ccaggaggca gatccagttc gtcgtgggcc
     2161 accagaattt cgtccggatt aatgcggaaa aaactggcca tcgccgcaac ggctttgccg
     2221 ctgagattca taaatgtagt cgggactaac aggcggacat cttcgcctcc aagagtgact
     2281 cgcgaagtat aaccaaagaa tttagcctct tcgcgcagcg gagcgcgcaa acgctctgcc
     2341 agtaagtcaa cgaaccaggc accagcatta tgtcgcgttg cggcgtattc agcaccgggg
     2401 ttcgccaggc cgacaatcaa tttaatcgtc acgttttttt gtcctgagtg tgtacataac
     2461 tggcgcgtag tttactggtt gcggccccgc ttgacaaaaa actgcgtatc aaatgcagat
     2521 aacgtaataa ttgcatgagt ggactattag aaagtcaagg tgttcaggcg tttatttgta
     2581 aagttttgtt gaaataaggg ttgtaattgt gatcacgccc gcacataacc cacagggtgt
     2641 tgtctatact ttacacataa ggaagagggg tattccctgt tacaacccag aaagttccgg
     2701 aggtgacata tgaaacgcaa aaacgcttcg ttactcggta acgtgctcat ggggttgggt
     2761 ctggtggtaa tggtggtcgg cgtggggtat tcaatcctca accagttacc acagtttaat
     2821 atgccccagt atttcgcaca tggtgcagtg ctaagtattt tcgtcggtgc cattctctgg
     2881 ctggcgggtg cccgtgttgg cgggcatgaa caggtgtgcg accgttactg gtgggttcgc
     2941 cactatgaca aacgttgccg ccgtagcgat aatcgccgtc atagctaaca aataatgcag
     3001 tttgctgacc agtcagtttg cgctgactgg tcaatctcgt acttataaat ccgccatcgc
     3061 cgcgcgacga ttcgggaaga acgccaggcg tcccgggatc ggttgaatgc cagcgcgagc
     3121 catagtgcgc agtggctgga attccacgtt gcacacgcgc agttcacatc cctcgggcag
     3181 acgcttcaca aaacgctgga acgcatcaag accaccagca tcaagtaccg gaaccgcatc
     3241 ccacttcaga atcacaatcc gtttgccttc aagacgtgac tccaggtccg tgaacaagcc
     3301 ttcagcagca gcaaaaaaca gcgggccaat aacgcgcaga accaggacat cgtctggaac
     3361 atctacgact accggtgcca ggcgagtcat acgtgcgata cgacgcataa acagcagcga
     3421 tgccagcacg atccccacgc tgatggcaat aaccatatca aacagcacgg tcagcgacat
     3481 gcacagcagc atgacaatga tgtcatcttt cggcgcatga cgcagcaagt cgaccacttt
     3541 gtgcgcttca ctcatgttcc acgccaccat caacagcagg gcagccatgg cggaaagcgg
     3601 cagccaggag agcagcggtg ccagtaccag cagggcaaga ataaccagaa tagagtggat
     3661 caccgccgag ataggggaag ttgccccggc acggacgtta gcggcagaac gcgcgatggc
     3721 agctgtagcg gtaatgccac caaagaacgg agcgatgata ttccccagtc cctgtccaac
     3781 cagttcgcta ttcgccttgt gtttcgtccc ggtcatacca tccagcacca cggcgcagag
     3841 cagagattcg attgcgccga gcattgccat tgagaatgcc gcaggcagca gtgtgcgaat
     3901 agaatcccag gttagcgtga attctgaatt aggcagatcc cacggcagca ccagttgcgg
     3961 cagcagttgc ggaataccgt taccctgaga accatcggcc aggacgtagt ggaattgcga
     4021 accgatggta gcaacatgtc cgccgagcag gttaacaatc cccatcaccg cgcaaccagc
     4081 cagcaaggcc ggaaggtgac cgggtaaacg aatgcccaga cgcggccaga aaacaagaat
     4141 acctagcgtc acaatgccaa tggcagcatc acccacatta atggtcggca gcgccataaa
     4201 taatgcgccg actttttgta gataatgttc cgggacatgg gccatttgca gaccgagaaa
     4261 atctttaatc tgcatggtac cgatggtgat cccaataccc gaggtgaaac ctaaggtgac
     4321 ggaaaccgga atatactcaa tcaggcgacc aaagcgtgcc agacccatca gaatcaaaaa
     4381 aatccccgac agcaaggtcg caaccagcag tcctgccagt ccaaactgtt gcgacacggg
     4441 atagagaatt accacaaatg ccgcagtcgg accggaaacg ctaaagcgtg acccacccgt
     4501 cagagcaatg acaatccccg caacagctgc ggtatataaa ccgtactggg gtgccacacc
     4561 actaccaata gccaacgcca tcgccagcgg gatagcaata atcccgacgg ttatcccggc
     4621 aatcaggtca cgggtaaacc gtgcggcagt atatttttct ttccagcaag cgtcgatcag
     4681 agcgcggaaa ggcatcacat gtgaggaaaa tattttgttc acaataatgt ttcatccgtg
     4741 agcgcatcat ctgtcaacta aatggcaggt gaaggaggca taggtcatac aaatggatat
     4801 tacagacaaa aaaacccgcc gcagcgggtc tttgagccgg gttcgattag tgttcgaaca
     4861 tggcagagat cgattcttcg ttgctgatac gacgaatcgc ttcggccagc atacctgaca
     4921 gggtcagagt acgcacgttc ggcagtgatt tgatttcatc gctcagcgga atggtatcgc
     4981 agacaacgac ttcatcaatt acagagttac gcaggttatt cgccgcgttg ccagagaaga
     5041 tcgggtgagt cgcgtacgca aatacacgtt tagcaccacg ttctttcaga gcttcagcag
     5101 ctttacacag cgtaccgcca gtgtcgatca tatcatcgac cagtacgcag tcacgacctg
     5161 caacgtcacc gatgatatgc atcacctggg aaacgttcgc acgcggacga cgtttgtcga
     5221 tgattgccat atcggtatcg ttcagcagct tagcgatagc gcgggcacgc acaacgccgc
     5281 cgatgtccgg agaaaccaca attgggttat ccagattcag ctgcagcatg tcttccagca
     5341 ggatcgggct accaaatacg ttatcaaccg gaacgtcgaa gaaaccctga atctgttcag
     5401 cgtgcagatc cactgtcagc acacggtcaa caccgacgct ggagaggaag tctgcaacca
     5461 ctttcgcagt gattggtaca cgagcggaac ggacgcgacg gtcctggcgc gcatagccaa
     5521 agtaggggat aacagcggtg atacgacctg cggaagcacg acgcagggca tcaaccataa
     5581 cgactaattc catcaggttg tcgttagtag gggcacaagt ggactggatg atgaaaatat
     5641 caccaccgcg tacattttcg ttgatttgta cactgacttc gccgtcgcta aagcgaccta
     5701 cagcggcgtc gccgagtgaa gtgtacaggc ggttggcaat acgttgtgct agttccgggg
     5761 tggcgttacc agcaaaaagc ttcatatcag gcacgagaag aacctcaggc atgcgtccat
     5821 tggtggaaag aatctgccga aaactgtgcg ggccaggcat gatcctttcc aggcggtgta
     5881 ttaaagagcg cgatgcaacg tctggaacaa ggtgacgttg tcaccgaaac tcagcttgcc
     5941 cggcttaaag catggctctg tgcaatgggg aaagattaac gcctttcgcc acaaagccat
     6001 tgagccattc cggggcttgc tctagcacct ggcgggcttc agactctgta tcaaattcag
     6061 caaagacaca ggcccctgtc ccagtcaggc gcgacggggc gtattctaac agccaggaaa
     6121 gcaccgcatc aacctcgcga aaacgttttc ttgcgataac ctcgcaatca ttgctgaatt
     6181 cacattttag caacgtttct attgaccttt ttggcgtatt gcgcgggagt tcaggatctt
     6241 taaaaatcac cggagtcgga atacttacac cagggtgcgc caccagatac cacttctctg
     6301 gcggatccac cggcgttagt atttcaccaa cgccttcggc aaacgaggca tgcccccgaa
     6361 caaagacagg aacatctgcg cccagcgtca gccccatttc cgccagctca tccatgctta
     6421 gcccgcattg ccagagatga tttaatgcca ccaggaccgt cgcggcattg gatgaaccac
     6481 cgccgagacc gccgcccatc ggcaaacgct tgtcaatgct gatattcgca ccgcttcccg
     6541 tcggaagacg cccgctgtct gccgcagttt tcatcaacaa tcgcgctgcg cgaacgatca
     6601 ggttatcttc atgttccacg ccttcaacgg gcgttaacag acgaatatcc ccatcgtcac
     6661 gaagctcaat gctgatggtg tcgccgtaat caagaaactg aaacagcgtt tgcagcgtgt
     6721 ggtaaccatc cgcacgctga ccggtaatgt ataaaaacag attaagtttt gccggagagg
     6781 gccactgtgt ccgcattatt tcactatcca gttatccatt tttaacttga tgcgttgacc
     6841 accgtcggtg agttccatat tggctggcat cgcaggttgc gttttggtgt cataaccacc
     6901 ataaacaacc ttccagtttt tgccattctg gctgtaggta atttcgctca ggcggtactg
     6961 gtcgtccagt ttgtagtcgg ttgcatcacc cggtaaacct aaaatccact ggcgcaagct
     7021 gttgagcgga attggcattc cggtcaattt gccaatcatc tcttcggcgt catcggcggt
     7081 ataacgctga cctttattgt cgactaactg cacgttaccc ggttgagcat tcagctccag
     7141 ttccgtgctg cccaatgggt tagtgagcag cagacggtag cgatcctggc cggtttgctg
     7201 ccagaaaaag cgggcgtaca ctttttgttg gtcagaaata taagcgaacg cgccgcgagt
     7261 ctgatactga ttaagattgc gcacgtcttg ctgatgctga cgccattgtg gcgaatccgg
     7321 gcttttgcca ggacctttgg gcgtggtaac ggaacaggca gtgagcacaa gagcagccag
     7381 cggtagcagg cggataagac gaaaatcggg caggggcata gtgatgacaa gtccttgaga
     7441 tacgttgcag ttataaccct taatgctagc gttaccgtcc gctatcgtct atgttcaagt
     7501 tgtcttaatt gccagaatct aacggctttc ggcaattact ccaaaagggg gcgctctctt
     7561 ttattgatct tacgcatcct gtatgatgca agcagactaa ccctatcaac gttggtatta
     7621 tttcccgcag acatgaccct tttagcactc ggtatcaacc ataaaacggc acctgtatcg
     7681 ctgcgagaac gtgtatcgtt ttcgccggat aagctcgatc aggcgcttga cagcctgctt
     7741 gcgcagccga tggtgcaggg cggcgtggtg ctgtcgacgt gcaaccgcac ggaactttat
     7801 cttagcgttg aagagcagga taacctgcaa gaggcgttaa tccgctggct ttgcgattat
     7861 cacaatctta atgaagaaga tctgcgtaaa agcctctact ggcatcagga taacgacgcg
     7921 gttagccatt taatgcgtgt tgccagcggc ctagattcac tggttcttgg ggagccgcag
     7981 atcctcggtc aggttaaaaa agcgtttgcc gattcgcaaa aaggtcatat gaaggccagc
     8041 gaactggaac gcatgttcca gaaatctttc tctgtagcga aacgcgttcg cactgaaaca
     8101 gatatcggtg ccagtgctgt gtctgtcgct tttgcggctt gtacgctggc gcggcagatc
     8161 tttgaatcgc tctctacggt cacagtgttg ctggtaggcg cgggcgaaac catcgagctg
     8221 gtagcgcgtc atctgcgcga acataaagta cagaagatga ttatcgccaa ccgcactcgc
     8281 gaacgtgccc aaattctggc agatgaagtt ggcgcggaag tgattgccct gagtgagatc
     8341 gacgaacgtc tgcgcgaagc cgatatcatc atcagttcca ccgccagccc gttaccgatt
     8401 atcgggaaag gcatggtgga gcgcgcatta aaaagccgtc gcaaccaacc aatgctgttg
     8461 gtggatattg ccgttccgcg cgatgttgag ccggaagttg gcaaactggc gaatgcttat
     8521 ctttatagtg tggacgatct gcaaagcatc atttcgcaca acctggcgca gcgtaaagcc
     8581 gcagcggttg aggcggaaac tattgtcgct caggaaacca gcgaatttat ggcgtggctg
     8641 cgagcacaaa gcgccagcga aaccattcgc gagtatcgca gccaggcaga gcacgttcgc
     8701 gatgagttaa ccgccaaagc gttagcggcc cttgagcagg gcggcgacgc gcaagccatt
     8761 atgcaggatc tggcatggaa actgactaac cgcttgatcc atgcgccaac gaaatcactt
     8821 caacaggccg cccgtgacgg ggataacgaa cgcctgaata ttctgcgcga cagcctcggg
     8881 ctggagtagc agtacatcat tttctttttt tacagggtgc atttacgcct atgaagcctt
     8941 ctatcgttgc caaactggaa gccctgcatg aacgccatga agaagttcag gcgttgctgg
     9001 gtgacgcgca aactatcgcc gaccaggaac gttttcgcgc attatcacgc gaatatgcgc
     9061 agttaagtga tgtttcgcgc tgttttaccg actggcaaca ggttcaggaa gatatcgaaa
     9121 ccgcacagat gatgctcgat gatcctgaaa tgcgtgagat ggcgcaggat gaactgcgcg
     9181 aagctaaaga aaaaagcgag caactggaac agcaattaca ggttctgtta ctgccaaaag
     9241 atcctgatga cgaacgtaac gccttcctcg aagtccgagc cggaaccggc ggcgacgaag
     9301 cggcgctgtt cgcaggcgat ctgttccgta tgtacagccg ttacgccgaa gcccgccgct
     9361 ggcgggtaga aatcatgagc gccagcgagg gtgaacatgg tggttataaa gagatcatcg
     9421 ccaaaattag cggtgatggt gtgtatggtc gtctgaaatt tgaatctggc ggtcatcgcg
     9481 tgcagcgtgt tcctgctacg gaatcgcagg gtcgtattca tacttctgct tgtaccgttg
     9541 cggtaatgcc agaactgcct gacgcagaac tgccggacat caacccagca gatttacgca
     9601 ttgatacttt ccgctcgtca ggggcgggtg gtcagcacgt taacaccacc gattcggcaa
     9661 ttcgtattac tcacttgccg accgggattg ttgttgaatg tcaggatgaa cgttcacaac
     9721 ataaaaacaa agcgaaggcg ctttcagtgc tcggtgctcg catccacgct gctgaaatgg
     9781 caaaacgcca acaggccgaa gcgtctaccc gtcgtaacct gctggggagt ggcgatcgca
     9841 gcgaccgtaa ccgtacttac aatttcccgc aggggcgcgt taccgatcac cgcatcaacc
     9901 tgacgctcta ccgcctggat gaagtgatgg aaggtaagct ggatatgctg attgaaccga
     9961 ttatccagga acatcaggcc gaccaactgg cggcgttgtc cgagcaggaa taatggaata
    10021 tcaacactgg ttacgtgaag caataagcca acttcaggcg agcgaaagcc cgcggcgtga
    10081 tgctgaaatc ctgctggagc atgttaccgg cagagggcgt acttttattc tcgcctttgg
    10141 tgaaacgcag ctgactgacg aacaatgtca gcaacttgat gcgctactga cacgtcgtcg
    10201 cgatggtgaa cccattgctc atttaaccgg ggtgcgagaa ttctggtcgt tgccgttatt
    10261 tgtttcgcca gcgaccttaa ttccgcgccc ggatacggag tgtctggtgg agcaggcact
    10321 ggcgcggttg cctgaacaac cttgccgtat tcttgatctc gggacgggta ccggggcgat
    10381 tgcgcttgcg ctggctagcg agcgcccgga ctgcgaaatt atcgctgtag atcgtatgcc
    10441 tgatgctgtc tccctggcac aacgtaatgc ccagcatctg gcgatcaaaa atatccacat
    10501 tctgcaaagc gactggttta gcgcgctagc cgggcagcag tttgcgatga ttgtcagcaa
    10561 tccgccgtat attgacgagc aggacccaca tcttcaacaa ggcgatgtcc gctttgagcc
    10621 gctcactgcg ctggttgcgg cagacagtgg aatggcagac atcgtgcata tcatcgaaca
    10681 gtcgcgtaac gcgctggtat ccggcggctt tctgcttctg gaacatggct ggcagcaggg
    10741 cgaagcggtg cgacaagcat ttatcctcgc ggggtatcat gacgtcgaaa cctgccgtga
    10801 ctatggtgat aacgagcgcg taacgctcgg ccgctattat caatgacaag tttttctaca
    10861 ctgcttagtg ttcatcttat tagtatcgcg ctttctgttg ggctattaac cttacgtttc
    10921 tggctacgtt atcagaagca tccacaggca tttgctcgct ggacgcgcat tgtgccgccg
    10981 gttgtcgata cggtgttatt gttaagcggc attgcgttga tggctaaagc gcacatcctg
    11041 ccattttccg ggcaggcaca gtggctgact gaaaagctgt ttggagttat catttatatc
    11101 gttttgggtt ttattgcact cgattatcgt cgtatgcaca gtcagcaggc gcgcattatt
    11161 gccttcccgc tggcgttggt ggtgctgtac atcatcatta aactcgccac cacaaaagta
    11221 ccgttactgg ggtaagtcat gagatcgtta gctgatttcg aatttaataa agcgccattg
    11281 tgcgaaggca tgatcctggc ttgcgaagca atccgccgcg attttccctc gcaagatgtt
    11341 tacgacgaac tggagcgtct cgttagtctg gcgaaggaag aaatcagcca gcttctgcct
    11401 ttagaagagc agctggaaaa actgatcgcg ctgttttacg gcgactgggg atttaaagcc
    11461 tcacgcggcg tttatcgtct ttccgatgca ttatggctgg accaggtgtt aaagaatcga
    11521 cagggcagtg cggtatcatt aggtgcggtt ttattatggg tcgcgaatcg tctcgatttg
    11581 ccgctgctgc cggtgatttt ccctacgcag ctgatattgc gcattgaatg tccggatggc
    11641 gaaatttggc tgattaatcc ttttaacggt gaatcgttaa gcgaacatat gctggacgta
    11701 tggttaaagg gaaatatcag cccgtcggcg gaactgtttt atgaagacct tgatgaagct
    11761 gataacattg aggtaatccg caaattgctg gatacactca aagcctcgtt gatggaagaa
    11821 aatcagatgg agctggcgtt acgcaccagc gaagctttat tacaattcaa ccctgaagat
    11881 ccctatgaaa ttcgcgatcg cgggttgatt tatgtgcaac tggattgcga acacgttgcg
    11941 ttgaacgatt taagttattt cgttgaacag tgtccggaag acccgatcag cgaaatgatc
    12001 cgtgcgcaaa taaataacat cgcgcataaa catattgtgc tgcattaatt aatcgacatt
    12061 ttactcaaga ttaaggcgat cctatgaaac aaaaagtggt tagcattggc gacatcaacg
    12121 tagcaaatga cctgccgttc gtactgtttg gcggtatgaa cgtgctggaa tctcgcgatc
    12181 tggcgatgcg catttgcgag cactacgtaa ctgtgaccca gaaactgggt atcccttacg
    12241 tgttcaaagc ctcttttgac aaagccaacc gctcctccat ccactcttat cgtggaccgg
    12301 gcctggaaga agggatgaaa atcttccagg agttgaagca gacttttggc gtgaaaatta
    12361 tcaccgacgt tcacgaacca agtcaggcac agcccgttgc tgatgtcgtg gatgtgattc
    12421 agttgccggc gtttcttgct cgccagactg acctggttga agccatggcg aaaaccggtg
    12481 cggtaattaa cgtcaagaaa ccacagtttg tcagcccggg acagatgggt aatatcgttg
    12541 ataaattcaa agaaggcggc aacgaaaaag tgattctttg cgatcgtggt gctaacttcg
    12601 gctatgacaa cctggttgtc gatatgctgg gcttcagcat tatgaagaaa gtgtctggta
    12661 actcgccggt gattttcgac gtgacccacg cactgcaatg ccgcgatccg tttggcgcag
    12721 cttccggtgg tcgtcgtgct caggtggctg agctggcacg agccggtatg gcggtaggtc
    12781 tggcggggct gtttattgaa gcgcatccgg atccggaaca tgcgaaatgt gatggtccat
    12841 ccgcgctgcc gctggctaaa ctggaaccgt tcctcaagca gatgaaagcg attgatgatc
    12901 tggtgaaagg tttcgaagaa ctggatacca gcaagtaatc ttttttgctt gaaaaataaa
    12961 gtcttagcgt tctgcgttaa gacttttttc atgggtgcca gatacaaaaa
//
LOCUS       DAZGLJ010000128        12860 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000128 DAZGLJ010000000
VERSION     DAZGLJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.128"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..870
                     /gene="yhfZ"
                     /locus_tag="ACHK49_003189"
     CDS             <1..870
                     /gene="yhfZ"
                     /locus_tag="ACHK49_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO9263798.1"
                     /translation="ITTLARYLLGEKCGNRLKTIDELATECRSSVGLTQAALKTLESS
                     GAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEGLASGLKAQFDGI
                     PFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGFCLALELGPHTYVGEHQL
                     ICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESLQRIVKGDVDAVI
                     WNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLLRAVVDKHALLAH
                     QQRVVSGEQEPSY"
     gene            887..1249
                     /gene="yhfY"
                     /locus_tag="ACHK49_003190"
     CDS             887..1249
                     /gene="yhfY"
                     /locus_tag="ACHK49_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO9263799.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            1333..2496
                     /gene="yhfX"
                     /locus_tag="ACHK49_003191"
     CDS             1333..2496
                     /gene="yhfX"
                     /locus_tag="ACHK49_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO9263800.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPMLAEYGVTHAEPGHALTGTIPANQQGDQPEYIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            2496..3722
                     /gene="yhfW"
                     /locus_tag="ACHK49_003192"
     CDS             2496..3722
                     /gene="yhfW"
                     /locus_tag="ACHK49_003192"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO9263801.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPALEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIGRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVHTQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            3719..4597
                     /gene="php"
                     /locus_tag="ACHK49_003193"
     CDS             3719..4597
                     /gene="php"
                     /locus_tag="ACHK49_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO9263802.1"
                     /translation="MSIDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            4608..4961
                     /gene="yhfU"
                     /locus_tag="ACHK49_003194"
     CDS             4608..4961
                     /gene="yhfU"
                     /locus_tag="ACHK49_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO9263803.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            4973..6277
                     /gene="yhfT"
                     /locus_tag="ACHK49_003195"
     CDS             4973..6277
                     /gene="yhfT"
                     /locus_tag="ACHK49_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO9263804.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            6289..7374
                     /gene="yhfS"
                     /locus_tag="ACHK49_003196"
     CDS             6289..7374
                     /gene="yhfS"
                     /locus_tag="ACHK49_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO9263805.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(7526..8257)
                     /gene="frlR"
                     /locus_tag="ACHK49_003197"
                     /gene_synonym="yhfR"
     CDS             complement(7526..8257)
                     /gene="frlR"
                     /locus_tag="ACHK49_003197"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO9263806.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(8357..9142)
                     /gene="frlD"
                     /locus_tag="ACHK49_003198"
     CDS             complement(8357..9142)
                     /gene="frlD"
                     /locus_tag="ACHK49_003198"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFO9263807.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSSLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLGENGSIAWDGAHFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(9139..9969)
                     /gene="frlC"
                     /locus_tag="ACHK49_003199"
     CDS             complement(9139..9969)
                     /gene="frlC"
                     /locus_tag="ACHK49_003199"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO9263808.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(10019..11041)
                     /gene="frlB"
                     /locus_tag="ACHK49_003200"
     CDS             complement(10019..11041)
                     /gene="frlB"
                     /locus_tag="ACHK49_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO9263809.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGQLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(11062..12399)
                     /gene="frlA"
                     /locus_tag="ACHK49_003201"
     CDS             complement(11062..12399)
                     /gene="frlA"
                     /locus_tag="ACHK49_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO9263810.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            complement(12694..>12860)
                     /gene="yhfL"
                     /locus_tag="ACHK49_003202"
     CDS             complement(12694..>12860)
                     /gene="yhfL"
                     /locus_tag="ACHK49_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO9263811.1"
                     /translation="NKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISK
                     IIGGCGPTAQ"
BASE COUNT         2951 a         3219 c         3652 g         3038 t
ORIGIN      
        1 atcacgacgc tggcccgtta tttgttgggt gaaaaatgcg gtaataggtt gaaaaccata
       61 gatgagctgg caactgaatg ccgttcatcc gttggcctga cgcaggccgc gttgaaaacg
      121 ctggaatcaa gcggagcgat acggattgaa cgccgtgggc gcaatggcag ttatctggtc
      181 gagatggata acaaagcatt gctgagtcat gtggatatca acaacgtggt atgtgcgatg
      241 cctttgccct atacccgctt gtacgaaggt ttagcgagcg gattgaaagc ccagtttgat
      301 ggcattcctt tttactatgc gcatatgcgc ggcgcggata ttcgcgtgga gtgtttactt
      361 aacggcgtgt atgacatggc ggtggtttcc cgactggcgg cggaaagtta tctcacgcaa
      421 aaagggtttt gcctcgcgct ggagttgggg ccgcacacct acgttggcga gcaccagttg
      481 atttgccgta aaggcgagtc cgcaaacgtg aagcgcgtgg ggctggataa ccgttcggcg
      541 gatcagaaaa tcatgaccga tgtctttttt ggcgatagtg atgtggaacg tgtcgatctc
      601 tcttatcacg agagtttaca acgcattgtt aaaggcgatg tcgatgcggt gatctggaac
      661 gtggtggcgg aaaacgaact gaccatgctg ggattagagg cgacgccgct cacagacgat
      721 ccgcgatttt tacaggccac cgaagccgtg gttctgacgc gagtcgatga ttacccaatg
      781 caacaactgc tgcgtgccgt tgtagataaa cacgccctgc tcgcccatca acaacgggta
      841 gtgagtgggg aacaggaacc gagttattaa acgaaaggca tctgatatgg aaaccagact
      901 caacctgctt tgcgaggcag gcgttattga taaggacatc tgcaaaggca tgatgcaggt
      961 cgtcaacgta ctggaaacag agtgccatct gccggtgcgc agtgagcaag gaacgatggc
     1021 gatgacacat atggcgagtg cactgatgcg cagtcgccgt ggtgaagaaa tagagccgct
     1081 ggataacgaa ttgctggcag agctggcgca atccagccac tggcaagccg ttgtgcaatt
     1141 gcatcaggtg ttgttgaagg aattcgcact ggaagttaac ccgtgtgaag aaggctattt
     1201 gctggccaac ctttatggat tatggatggc tgctaacgaa gaggtttgaa gctcccgaca
     1261 aatccacgtc gcacaaccta aatattcaaa aaaatgaata tttaattcaa ggaacagaaa
     1321 cgaggcaaca agatgtttgt agaagcattg aaacgccaga acccggcgct gatttccgcc
     1381 gcactaagtt tgtggcagca gggcaagatc gccccggaca gctgggtgat cgacgtggat
     1441 caggtactgg aaaacggtaa gcgactgatt gagacggcgc ggctttacgg cattgaactg
     1501 tatctgatga ccaagcagtt tggccgtaac ccatggctgg cggaaaaatt gctggcatta
     1561 ggttacagcg gcattgtggc ggtggattac aaagaggcgc gagtcatgcg ccgcgctggt
     1621 ttgtcggtgg cgcatcaggg gcatctggta caaatccctt gtcatcaggt tgctgacgcc
     1681 gttgaacagg gcaccgacgt catcaccgtg tttactctcg acaaagcgcg ggaagtttct
     1741 gcggcagcgg tgaaggccgg acgtgtgcag tttgtgctgc tcaaagttta tagcgacgat
     1801 gattttcttt atccggggca ggagagcggt tttgtacagc attcgctgca cgaggtggtt
     1861 gctgaaatta agaaactgcc gggtctgcat ttagccggac ttacccattt cccttgcctg
     1921 ctttgggatg aggctgccgg gaaagttttg ccgacaccga atcttcacac gctggtacaa
     1981 gcacgggatc aactggcgaa atctggtatt gcgattgagc aactgaacgc gccttctgcg
     2041 accagctgca cttcgctgcc gatgctggcg gaatacggtg tgactcatgc cgaacccggt
     2101 catgcgctga cgggcactat tccggcaaac cagcagggcg atcagcctga atatatagcg
     2161 atgctttggt taagtgaaat ctcccatcat ttccgtggcg acagctactg ctacggcggc
     2221 ggttactacc gtcgtggtca tgcgcaacat gcgctggtgt ttacgccaga aaatcaaaag
     2281 attactgaaa ccaatctcaa aactgtggat gacagcagta tcgactacac cctgccgctg
     2341 gcaggcgagt ttccggtaag cagtgcagta gtgctctgtt ttcgcacgca gatttttgtc
     2401 actcgtagcg atgtggtgct ggtgtccggt attcaccgtg gcgaaccgaa aatcgtcggt
     2461 cgttatgaca gtcttggaaa ctctctgggg gcgtaatggc gcgatttgtg gtgttagtga
     2521 ttgatagctt tggcgtaggg gcaatgaaag atgtcacgct ggtgcgcccg caagatgccg
     2581 gggcgaatac ctgtggtcac atcctgagcc agttgccgca tttgcagcta ccagcgctgg
     2641 agaagctggg gctaatcaac gcattgggtt atgcaccagg tgatatgcaa ccgtcagatt
     2701 ccgcaacctg gggggtggca gagctgcaac atgaaggtgg cgataccttt atggggcatc
     2761 aggaaatttt aggcacgcgc ccgttaccgc cgctgcggat gccctttcgc gatgtgattg
     2821 gccgtgttga gcaggcatta gtttccgctg gctggcaggt ggagcgccgt ggcgatgatc
     2881 tgcaatttct gtgggtcaat caggcggttg cgattggcga taatctcgag gcggatttag
     2941 gccaggtcta taacattacc gccaatctct ctgtgatctc ttttgacgac gcgatcaaaa
     3001 ttggtcgtat cgtgcgtgag caggtacagg tcggtcgggt cattacattt ggcggcttgt
     3061 taaccgacag tcagcgtatt ctcgatgccg cagaaagcaa agaagggcgc tttattggta
     3121 ttaatgcgcc gcgttctggc gcttatgaca acggcttcca ggtcgtgcat atgggctacg
     3181 gtgttgatga aaaagtgcag gtgccgcaaa aactgtatga ggcgggcgtg ccaaccgtgc
     3241 tggtaggtaa ggtggcagat atcgtcagca atccttatgg cgtgagctgg caaaacctgg
     3301 tggatagcca gcggattatg gatatcaccc tcgacgaatt taacacgcat ccgacggcgt
     3361 ttatttgcat caatatccag gaaaccgacc tcgctggtca tgcagaagac gtcgcacgtt
     3421 atgccgaacg tttgcaggtc gttgaccgta accttgcccg gcttgttgag gcgatgcagc
     3481 cagatgattg cctggtcgtg atggcggatc acggtaacga tccgaccatt ggtcacagcc
     3541 accatacccg tgaagtggtg ccagtgctgg tttatcagca agggctggtc cacacgcaac
     3601 tcggtgtacg caccacgctt tctgatgtgg gggctaccgt gtgtgaattt ttccgtgcgc
     3661 caccgccaca aaatggtcgc tcttttcttt cctccctccg gtttgcagga gacaccctat
     3721 gagtattgat ccgacgggtt acaccctggc ccatgagcat ctgcatattg atctctccgg
     3781 ctttaaaaac aacgtggact gccgccttga tcagtatgcg ttcatttgcc aggagatgaa
     3841 cgacctgatg acccggggcg tgcgtaatgt gattgagatg accaaccgtt acatggggcg
     3901 caatgcgcaa tttatgctcg atgtaatgcg cgagacgggg atcaacgtgg tggcctgcac
     3961 cggttattac caggacgctt ttttcccgga gcatgtggcg acccgcagcg tgcaggaact
     4021 ggcgcaggag atggtcgatg aaattgaaca gggtatcgat ggcaccgacc tgaaagccgg
     4081 gattatcgcg gagatcggct ctagcgaagg gaagattacg ccgctggaag agaaggtgtt
     4141 tattgccgct gcgctggcgc ataaccagac cggacgcccg atctccacgc atacttcgtt
     4201 cagcaccatg gggctggaac aactggctct gttgcaagcc cacggagttg atctttcgcg
     4261 cgtcaccgtt ggtcactgcg atctgaaaga caatctcgac aacattttga agatgatcga
     4321 tctcggcgcg tacgtgcagt tcgacaccat tggtaagaac agttactacc cggacgaaaa
     4381 gcgcattgcg atgctccacg cgctacgtga ccgtgggttg ctgaaccgcg tcatgctgtc
     4441 gatggatatt acgcgccgct cccatttaaa agccaatggt ggttatggct atgactattt
     4501 attgaccacc tttattccgc aattgcgcca gtcaggattc agtcaggccg atgtggatgt
     4561 gatgttacgt gaaaatccct ctcaattttt ccaataagga cagactcatg aaaaagattg
     4621 gcgttgcagg cttacagcgt gagcagatta aaaaaaccat tgaagcgacg gctcctggca
     4681 gttttgaagt tttcattcac aacgacatgg aagcggcaat gaaagtgaaa tccgggcaac
     4741 tggattacta catcggtgcg tgtaataccg gtgcgggcgc ggcattgtcg attgccatcg
     4801 cggtgattgg ctataacaaa agttgcacca ttgccaaacc aggcattaaa gcgaaagacg
     4861 agcatatcgc caaaatgatc gctgaaggga aagtggcgtt tggcctttcc gttgagcacg
     4921 tcgaacacgc gattccgatg ctgattaacc atctgaaata aaaggcacga ctatggatct
     4981 gtatattcag attatcgtgg tggcgtgcct gacgggtatg acatcgcttc tggcgcatcg
     5041 ctcggcggct gtttttcatg acggcatccg cccgatcctg ccgcaactga ttgaaggcta
     5101 tatgaatcgt cgcgaggcgg ggagtatcgc ttttggtctg agcattggtt ttgtggcctc
     5161 ggtggggatc tcttttaccc tgaaaaccgg gctgctcaac gcatggttac tctttcttcc
     5221 taccgatatc ctcggcgtac tggcgataaa cagcctgatg gcgtttggtc ttggcgctat
     5281 ctggggcgtg ttgatcctta cttgcctgtt gccagtaaac cagctgctga ccgcgctgcc
     5341 ggtggatgta ttaggtagcc tcggggaatt aagctcgccg gtggtttctg cttttgcact
     5401 gttcccgctg gtggcgattt tctaccagtt tggctggaag caaagtctgg tcgccgccgt
     5461 ggttgttctg atgacccgtg tggtagtcgt gcgctatttc ccacatctta accctgaatc
     5521 catcgaaatc tttattggca tggtgatgct gctggggatc gcgataactc acgacctgcg
     5581 tcatcgtgat gaaaatgaca tcgatgccag cgggctttcg gtgtttgaag agcgcacgtc
     5641 gcggattatc aaaaacttac cttatatcgc catcgtggga gcattgattg ccgccgttgc
     5701 cagtatgaag atttttgctg gtagcgaagt ttcgatcttc acactggaga aagcgtattc
     5761 cgcaggcgtg acgccggaac aatcgcaaac gctgattaat caggcggctc tggcagaatt
     5821 tatgcgcgga ctgggttttg tgccgttgat tgccaccacc gcgttagcca ctggcgtgta
     5881 tgcagttgcg ggctttacct ttgtttatgc ggtgggctat ctctcgccaa atccgatggt
     5941 tgcggcggta ttaggcgcag tggttatttc ggcggaagtc ttgctgcttc gttcgatcgg
     6001 caaatggctg ggacgctacc cgtcggtgcg taatgcgtcg gataacatcc gtaacgccat
     6061 gaatatgctg atggaagtgg cgctgctggt cggttcgatt ttcgcagcaa ttaagatggc
     6121 gggttatacc ggattctcta tcgcggttgc catttacttc ctcaacgaat ccctgggccg
     6181 tccggtacag aaaatggcgg caccggtcgt ggcagtaatg atcaccggta ttctgctgaa
     6241 tgttctttac tggcttggcc tgttcgttcc ggcttaagga aggcacctat gaagacgttt
     6301 cctctgcaaa gcctgacgat tattgaggcg cagcaaaagc agtttgcgct ggtggatagc
     6361 atttgtcgcc atttccccgg cagcgagttt cttactggcg gtgatttagg gttaactcca
     6421 ggtctgaatc aaccgcgtat tacccaacgg gtggagcagg tgctggctga tgcatttcac
     6481 gcacaggctg cggcgctggt gcagggcgcg gggactggcg cgattcgcgc cgggctggcg
     6541 gctttgctca aaccggggca gcgtcttctg gtgcatgacg cgcctgttta cccgacgaca
     6601 cgggttatta ttgagcagat ggggctgacg cttattactg ttgatttcaa tgacctgtcg
     6661 gcactgaagc aggtcgtcga cgagcaacaa ccggatgcgg cgctggtgca gcatacgcgc
     6721 cagcagccgc aggacagcta cgtgctggca gatgtgctgg caacgttgcg cgcggcaggt
     6781 gttccagcgt taaccgatga caactatgcg gtgatgaagg tggcgcgaat cggctgtgaa
     6841 tgcggcgcga atgtctcgac attttcctgc ttcaagctat ttgggccaga gggtgttggt
     6901 gcagtggtcg gcgatgctga tgttatcaac cgtattcgcg ccacgcttta ctccggcggt
     6961 agccagatcc agggcgcaca ggcgctggaa gtattgcgtg gtctggtgtt tgcgccagtg
     7021 atgcacgcgg tgcaggcagg ggtatctgaa cggttgctgg ctttgcttaa cggtggtgcg
     7081 gtgccggaag tgaaaagcgc ggtgattgct aatgcgcagt cgaaggtgtt gattgtcgag
     7141 tttcatcagc cgattgccgc cagagtgctg gaagaggcgc aaaagcgcgg tgccttgcct
     7201 tacccggtgg gtgcagagtc gaaatatgaa atcccgccgc tcttttatcg cctttccgga
     7261 acgtttcgcc aggcgaatcc acaatcagaa cattgtgcga ttcgcattaa cccgaatcgc
     7321 agcggtgaag agacggtgct gcggattttg cgtgagagta ttgccagtat ttaatgctgg
     7381 atagcaccgt caagctaaat tccgtactga acggtcccct cgcccctttg gggagagggt
     7441 tagggtgagg ggaacgtacg ggatgtaaaa ttcaacgcgc cagcatcgcc ggatgcgatg
     7501 ctggcgcatc ttatccggcc tacggttaat gtcttttatt atcaatcgtt aaggtgatgc
     7561 gattggcgcg gcagaagagt tcggagacgt gcaccggttt gtcattctga tcaaaggcga
     7621 ttttgctgat gcgaaacaac ggttcgccca gttcgcattt cagccatttt gcctgcgggc
     7681 gggtggcggc aaagatatcg atggtctttt tgtcgctgac cactcgcgtg tcgaaacgct
     7741 cctgaaataa ctgataagtg gagcttcctt caacgtaaat ctcatcaaag tcaggataac
     7801 gcgacagtgg gatccaggaa ctatcaataa acagcggctc tttatcgaga tacatcaccc
     7861 ggcaaagatg gaacacttcg ctgttgccag ggatgttcag cttttcgcaa aacggcgcgg
     7921 cgctgactcg ttcctgctcg atcactttct ctttcgtcgc cttgccttgt gagacgccaa
     7981 aatcggtaaa accactgacg gtcagcaggg cgttttcgac cttctggctt tgtacaaagg
     8041 tgcctttgcc ctgccagcgg atcagtacgc cgtctgccac taagtcgctg atagctttgc
     8101 gaatagtaat gcggctgacg ttatattgcg tacaaagctc gttttcggta gggatctgtt
     8161 gcccggcctg gtaaaccccc tgcgcgatat catccagcaa ccgctggcgg acggtagcgt
     8221 agaggagttg atgagagtag cggtccgtag ctgacataat cgaccctgaa ataaagaatg
     8281 aattactcgg attatagggt cgttttccgg caagtaacag cacgttggcg tgaagatgct
     8341 cacgccaacg ttatacctac caggcaccgt ggtactgaat ggttttcgcc gcgcacgccg
     8401 ttccctgcgc catcgcctgc ggtaatgtca tccccgcaga ccagccgcaa aggaatccgg
     8461 caatgaacga atctccggca cccatggtgt cgataaccgt caccggttca ggagcctgac
     8521 gccagaagtg cgcgccatcc caggcaatgc tgccgttttc acccagcgtg acaatcacca
     8581 cgcctgcgcc acgggcaaca atcgctttca tcttcagacg cagcgcttcg tcttcttgcg
     8641 gtgcggaggc aaaggcaaaa tcgagatgcg gcaccagtgt ctgccagagc gagctgtccc
     8701 acttgtcgga gaagtcgaaa gcggtaagtt tgcccgcagc gtgcagctgt gggaatgcgt
     8761 cttccgcatg tccccagatt gccgcgtgca caatgtcata ctgcgccagc caggcgtaat
     8821 cctcttcact cagggcaaag tcggccatca cgccttcggt gtagtcgcca aaaacgcgat
     8881 cattgtcgtg cagttccacc tgagtttgtg cggtaacgcc gtgtttcgta tggacatggc
     8941 tgatatcgac gcccatgcgg gcgagatcct gcttcagctt tgtgccgtag tcatcgtcac
     9001 ccacccaggt aatgcatccc ggctgtatgc cgtagcgagt gcagtacacc gccacattga
     9061 ccgcattacc gccagaaaac gctttattca gttgcgggta gatatcgacg cagttatcgc
     9121 cgattgtcgc cagggttttc atctctcatc ctccggcagc agcgcgcgaa agcgttccag
     9181 cgcctggcgg gcatagagtc tgggctcgtt catatacatc gtcaccagct ccaccgtaca
     9241 gtaaccctca tagccccggt cgataatatc gcgcatcagt tccctcagcg gcattttgcc
     9301 ttctccagga atgtaatgcg tgtcgctggc cccgtcgctg tcgacaatat gcagatgacg
     9361 taatttatcg cccagtttgt cgaaataact catcaccggt tccgcctgga catacggcgc
     9421 gcaaatgtcg accatgctga acaggcgcgg cgaaggcacc agcgccagcg catgaagcac
     9481 atcattagca ttacacacga cgttcgattc atacggcgtt aacggttcga ggattagatc
     9541 catgccgatg ttttccgcgt actcacacag ttcgcttagg ttctctgcca gccgtcccca
     9601 gataacatta ggtggcgtga gatagcccgc gtgggccgcg gaaatcagcg tataacccgc
     9661 gttcatctct tttgccatat ccatcgccag cttgatcatg tcgaggcttt cgcgacgcat
     9721 atgttcatcg cccagcatca tgttatacgg atagccgttg gtttctggcg tatagccgat
     9781 aatcggcatc tgatacgtct gcgccagcgc cttgatttgt ttgatgccgc ccgcttttaa
     9841 gtccggcgcg aacgcgtgcg ggcgaccgcc ccaaatttcg atgccgtcgt aacccagctc
     9901 gcttgcatca cgaaatgcgt gttcaatagg cagccgctgg tggccgcagg taaacatacc
     9961 tgttttcatc gggtaactcc ttgcgtaaat tcggcccgtt gccgggccgg gagagggatt
    10021 aatattccac cagaccaccg taatagcggc gttcatccgg gttgtgatct ttgtaaatag
    10081 acaggtagta gcagagccac tccattggca cgaacatcag gaacggtgcc agccacgggt
    10141 gcagcccttg cgaaatttcg gcgtaatcga tgacgatcac gttgtcagta cgctgtttaa
    10201 caaagttaat ggcgcgttcg gtggtgtggc gactttcatc attgccgagc aggaacagga
    10261 acggaacgcc cggttcgaca atctccagcg ggccatggcg gaactctccg ctctcaatca
    10321 cgcagccgtg cgtccaggta aattccatca gcgttacaat gccttctttg taacccagcg
    10381 gacgcagcgg acccgcagca acggtataaa tcatcggcca ctggctggcc agttcaccaa
    10441 gctggcggcc tttttcttcc caggtgcgta ccagttgacc gagcgcattc ggcaactgct
    10501 tgagatcgtt tttgatcttg ccgatttccg cgttcggcgc gaggcgggtg atcatctcca
    10561 gcaccacgct gtagcagagc agcaggtgaa tttcccagat acagtcggcc tgataatcaa
    10621 tgctaaattc cgccgccgag gtaatcgggc tgtccgcgcg tttggtgaac gccgcagtga
    10681 gtgcgccgca ggcccggccc agctccagcg ctttgattac ctcttccgtt ttaccgtagt
    10741 cagaaacgcc aatcaccgcg caacgatcgt cgaggcgata cggggtgtta tcgcagaact
    10801 cccagccgga aatggcgtag acctgaagat cggaaaagcg atccgccaga tgtttcgccg
    10861 tttgcgccgc gttgagtggc gatccgcagg caacgaaata aatacgatca atgtcgcgct
    10921 tcaccatctc ttccacgatg gtgtgcacca gcggaacgtc atggctgaga actttttcca
    10981 cttcctgaac catattttcg gtgaccagaa agtccacggt gcttttatca atatccaaca
    11041 ttttattttc tccagataac attacgacaa agcgttgagc tggcggctac gcttcgccca
    11101 gaaggcgtaa gcaggcagac cagtagcaat aacgatgacg gcgcagataa ggccgggaat
    11161 cggtgcccag acaaaggttg aggcgaccag aatgaggctt gacgcaatgg cgagggtggt
    11221 catcagcccg aaagccggag tacgccacag cggtttgtaa tcgtcgcgtt tacgacacca
    11281 gatgatggag ccgaaggtga gggtattttt gaaacacatc accagggtga aataacccag
    11341 caggctggtg agatcggaaa cgaagatgaa gaagatcccc agcgcccctt gcaggatgat
    11401 ggagacatcc ggcgtgttgt atttcggatg cacatggccg aagcatttaa agaacaggtt
    11461 gtctttcgcc atcgcgtatt ccagccgcgg ctggtacatc acgcagctgg aaagcgaacc
    11521 aagaatgacg atcatcgccg tgatggcaac aaagatccca gcggtgctgc cgagtgcggg
    11581 gatccaggtc agggcgtcgg aaatcggcgt ttcagaattg gcgagtttgt cgaagggcat
    11641 caggccggaa atcaccagcg ccagcagggt gtagagcacc agaaccagca gacaggaacc
    11701 aatcagcgct cgtggcatgg tttttccggg gtttttaatt tcgccggtca tataacagat
    11761 agaggccatg ccggtatacg accaactggt ggcagagatc cccgccagca gcgccataaa
    11821 gctgcccgtt gcgccaatcg cagtggtggt aggggcggca aaattctccg ctttaaacca
    11881 gaagatccca aggccaatga cgatagtgaa cgggataatt ttggcgatgg taattagcgt
    11941 ctgaaacgct gcgccgcctt caacggagcg caggtgtagc aacataaagg cgataattaa
    12001 tccggcggcg ataaatttac cgagcaacgg atcgataggc gttaaaaagc caagattgct
    12061 gacaatcgcc agcgccataa tcgacaatga cggcgcatcg ttggcccaga agctggccca
    12121 gccggagagg aaagccagcg gtcggcttcc ggcatttttc agataaacat aatctgcgcc
    12181 attttccgga taagcggtgg atagttccgc atagacgcac atttgcggga tcacaattaa
    12241 cccgccaatg acaaacgcga gcaccgtaag ccacggcgtg cccgctgctt ttgccacttc
    12301 acccacagat acaaaaatac cggagccgac ggttgtcccg acggcgattg caagaacggc
    12361 ccaaaatccg agcttgcgtt ggagttcctg gcttcccata gcgatacctt tttcttgtcc
    12421 aatacgttgt aggggagagt ttgcagtcat ttaaaaggta acatgacaat gcatgatgaa
    12481 tataacaaca tatgatgtta tgtaatgtgg aggaagatca caaaatttcg cacaggatcg
    12541 cgctgtggct aatggatgta gttatcaaat tgaatttaaa gtgaaaatat ttttacgggc
    12601 gggggcaaga aggacataga aacaaatacg ccctcggaaa atccagaggg cgtcgggcaa
    12661 ttaaaccggt gttagccgat ttctgtcaga gacttactgt gcagtaggac cgcaaccgcc
    12721 aatgatttta gaaatagaaa ttgccgggtg cagcaggtag tcgtaagagc agttcttatc
    12781 acggttttca atatgaccag tacatgcagt taacgtagcc agtactgcac ctaccagtgc
    12841 aactttaata aatttgttca
//
LOCUS       DAZGLJ010000129        12821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000129 DAZGLJ010000000
VERSION     DAZGLJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.129"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..729
                     /gene="ybjI"
                     /locus_tag="ACHK49_003203"
     CDS             <1..729
                     /gene="ybjI"
                     /locus_tag="ACHK49_003203"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO9263812.1"
                     /translation="YQQMKAQGIRFVVASGNQYYQLISFFPEIANEIAFVAENGGWVV
                     SEGKDVFNGELSKDAFATVVEHLLTRPEVEIIACGKNSAYTLKKYDDAMKTVAEMYYH
                     RLEYVDNFDNLEDIFFKFGLNLSDELIPQVQKALHEAIGDIMVPVHTGNGSIDLIIPG
                     VHKANGLRQLQKLWGIDDSEVVVFGDGGNDIEMLRQAGFSFAMENAGSAVVAAAKYRA
                     GSNNREGVLDVIDKVLKHEAPFDQ"
     gene            815..1099
                     /gene="ybjH"
                     /locus_tag="ACHK49_003204"
     CDS             815..1099
                     /gene="ybjH"
                     /locus_tag="ACHK49_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO9263813.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(1140..2372)
                     /gene="mdfA"
                     /locus_tag="ACHK49_003205"
     CDS             complement(1140..2372)
                     /gene="mdfA"
                     /locus_tag="ACHK49_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO9263814.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            2657..3253
                     /gene="ybjG"
                     /locus_tag="ACHK49_003206"
     CDS             2657..3253
                     /gene="ybjG"
                     /locus_tag="ACHK49_003206"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO9263815.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            3311..4069
                     /gene="deoR"
                     /locus_tag="ACHK49_003207"
     CDS             3311..4069
                     /gene="deoR"
                     /locus_tag="ACHK49_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO9263816.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(4116..5318)
                     /gene="dacC"
                     /locus_tag="ACHK49_003208"
     CDS             complement(4116..5318)
                     /gene="dacC"
                     /locus_tag="ACHK49_003208"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO9263817.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            5565..6191
                     /gene="gstB"
                     /locus_tag="ACHK49_003209"
     CDS             5565..6191
                     /gene="gstB"
                     /locus_tag="ACHK49_003209"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO9263818.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(6188..7303)
                     /gene="yliI"
                     /locus_tag="ACHK49_003210"
     CDS             complement(6188..7303)
                     /gene="yliI"
                     /locus_tag="ACHK49_003210"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO9263819.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(7414..7797)
                     /gene="bssR"
                     /locus_tag="ACHK49_003211"
     CDS             complement(7414..7797)
                     /gene="bssR"
                     /locus_tag="ACHK49_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO9263820.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            8010..9335
                     /gene="rimO"
                     /locus_tag="ACHK49_003212"
     CDS             8010..9335
                     /gene="rimO"
                     /locus_tag="ACHK49_003212"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO9263821.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(9382..10710)
                     /locus_tag="ACHK49_003213"
     CDS             complement(9382..10710)
                     /locus_tag="ACHK49_003213"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO9263822.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYYAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(10718..>12821)
                     /locus_tag="ACHK49_003214"
     CDS             complement(10718..>12821)
                     /locus_tag="ACHK49_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO9263823.1"
                     /translation="RAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQV
                     NGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGK
                     DHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYNSE
                     GNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLV
                     VYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKD
                     AEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDER
                     NKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRN
                     LRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIAR
                     NIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFI
                     NELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLK
                     ANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIK
                     VDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLY
                     QKPMPFSAWDKSGKLVKE"
BASE COUNT         3369 a         3234 c         2954 g         3264 t
ORIGIN      
        1 tatcagcaaa tgaaagcaca aggcattcgc tttgtggtcg ccagcgggaa tcaatattat
       61 cagttgatct ctttcttccc tgaaattgcg aatgaaattg cctttgtggc tgaaaacggc
      121 ggctgggtag tgagtgaagg caaagatgtt tttaatggcg agctgtcgaa ggatgcgttt
      181 gctactgtcg tggaacattt gctgacgcgc ccggaagtgg aaattattgc ctgcggaaaa
      241 aatagcgcct atacactcaa aaaatatgac gatgccatga aaacggtggc ggaaatgtat
      301 tatcaccgtc tggaatacgt cgataacttt gacaacttag aagatatttt ctttaagttt
      361 ggcctgaatc tttccgatga actgattcca caagtacaaa aagcattaca tgaggccatc
      421 ggcgatatta tggtgccagt ccacaccggc aacggcagca tcgatctgat tatccccggc
      481 gtacataaag ccaatggcct tcgccaactg cagaaattat ggggaataga cgacagcgaa
      541 gtggtggtct ttggcgatgg cggtaacgat attgagatgc tgcgtcaggc aggctttagt
      601 tttgcaatgg aaaatgccgg cagcgcggtc gtcgcagcgg caaaataccg cgcaggctcc
      661 aataaccgtg aaggcgtact ggatgtgatc gataaagttc ttaaacacga agcgccattt
      721 gaccaataaa aacggcagca cgcccggcaa cacaatgcct gaatgaaggc tgcataattc
      781 gcggaaatta acccgctgca ttatttcagg cgaaatgatt atgaaaaatt gtctactgtt
      841 gggcgcgctt ttaatgggct ttactggtgt ggcgatggcg caaagtgtca ccgtcgatgt
      901 gccgagtgga tacaaagtgg ttgtggttcc tgattcagtc agcgttccac aagcggtcag
      961 cgtcgcgact gtgccgcaaa ccgtctatgt ggctcctgcg ccagctccgg cttaccgccc
     1021 gcatccttat gttcgccatc ttgccagcgt aggtgaaggg atggttatcg aacaccagat
     1081 tgacgaccat caccattaat tcgagaatgc ctgatcgcac aaagcaatca ggcatttttt
     1141 tacccttcgt gagaatttcc catctgttta tcttttaaaa agataaccat cagcgacagc
     1201 cacaaaattc cgttgacaag gttgaagaga ttaaacagtc cgttgccccc gttcagccag
     1261 gcatgtttgc tgatttcaat accgacggta aagatcagca tttgcagcat tcccatcgcc
     1321 gcagaaaccg tacctttact catatcgctg gcaaacaggg ttaatcgcac cagtcccgca
     1381 ttcgccagac caatgccgaa agcataaata cttaacccgg cggtcatcca taaatacgcg
     1441 tgcgatgaga taaccgttgc cgcagcagcg accaacagac caatcataat cggccagccg
     1501 cccataatta tcagcgaacg tacggtgcgg cgcgaggtca aacgcgctaa caacaagtta
     1561 cccgcaatta acgccccgaa aataggcact tgcagcaagc catattcata gctgctcaac
     1621 tgctcgccgg taatgatgat aatcggcgac tgggcgatcc acgccagcaa tggcaggcta
     1681 acgaatccca gcgccagcgc ccccgccaca aagcggccgt tcttcagcac cagcttatag
     1741 tcacgaccga gttctttcag tgacagtttc tcgcctatac gcgtggcggt ttcaggcatg
     1801 gcttgttgca gaccgaaaaa ggagatcgct gccaatgcgg caaacaagac aaacatccct
     1861 tcccagggca gcacatggat ccacgccgcg cccaccagcg gaccaagtag cggagcaatc
     1921 agcgccacgt tcgccatcag cgcggtgatc ttgatacaaa ccgcctcttc gaaggattcc
     1981 tgaattgcgg cgtatcccac agcgccaatg aaacagaggc ttatgccctg caagaagcgc
     2041 aacagagtga attgttcaat gttttgcgcc agtaatattg ccagacaggt gatgataaac
     2101 cacaccaccc ccgccagcat caccggacgg cgaccaatac gatccgacag cggccccagg
     2161 agccattgta aaaacatccc gcccgccaga tatgcggtca ttgaagtagg aacccaatca
     2221 atgcccgcct gatattgttc caccacggcc aacataccgg gttgaatcat atcgttgccg
     2281 atataggttg aaaattcgta aagcaccaga cagagaggga aaagtaacgc ctgacgtcca
     2341 agcctggcac cggaagctaa tttattttgc atgcaatttc ttcgccaata ataatcgcgc
     2401 agagtttaat aaaagcacag ctaacgagaa agcgaatttt gtagctgaaa ccacggttaa
     2461 gcacattctt acattattgc gagtatagct acgctttctt taagttttat ttaacctatg
     2521 cccgttacaa ttacccaccg taaacaggcc gcttgaggga aataagacga tgccgcttta
     2581 cccagtttaa cctgcacttt attctcaacg acttgcctgt attggctccc ttttaatcac
     2641 tttgcgtcgg gaagttatgc tggaaaattt gaatctctct ctattctctc ttattaacgc
     2701 gacgccagac tcggctccgt ggatgatctc gttggcgatt tttattgcta aagatttgat
     2761 taccgtggta ccgctgctgg ccgcggtgct ttggttgtgg gggcttacag cacaacggca
     2821 actggtgata aaaatcgcta tcgcgctggc ggtcagcctg tttgtgtcct ggacgatggg
     2881 acatcttttt ccgcacgacc gaccctttgt cgaaaatatc ggctataact tcctgcatca
     2941 tgcggcggat gactcattcc caagcgacca cggtacggtg attttcacct ttgcactggc
     3001 atttttatgc tggcatcgcc tgtggtccgg ctcactttta atggtgctgg ccgtcgtcat
     3061 tgcctggtcg cgcgtttatc ttggcgtgca ctggccgctg gatatgctcg gtggattgct
     3121 ggctggtatg attggctgcc ttagcgctca gattatctgg caagcgatgg ggcataaact
     3181 ctatcaacgt ctgcaatcgt ggtatcgctt ctgttttgca ttaccgatcc gcaaaggctg
     3241 ggtgcgtgac tgatctctgc taaaaagtgt agtattgagc ggctcgcttc aataactatt
     3301 cagagggatt atggaaacac gtcgcgaaga gcgcatcggg cagctgctgc aagaattaaa
     3361 acgcagcgat aagttacatc ttaaagacgc cgccgccctg cttggggttt cggagatgac
     3421 gattcgtcgc gatctgaaca accacagtgc gcccgtcgtt ttgctcggcg gctatattgt
     3481 tctggaaccg cgcagtgcca gccattacct gttaagcgat caaaaatccc gcctggtgga
     3541 agaaaaacgc cgggcggcaa aaatggctgc gacactggta gaacccgatc agactctctt
     3601 ttttgactgt ggcaccacca cgccgtggat tattgaagcg attgataatg aaatcccttt
     3661 taccgccgtt tgttattcgc taaatacctt tctggcgctg aaagagaaac cccattgccg
     3721 cgcatttctg tgcggtggtg aatttcacgc cagcaacgcc attttcaaac ccatcgattt
     3781 tcagcaaacg ttgaataatt tttgcccgga tatcgctttt tattccgcgg cgggcgtgca
     3841 tgtcagtaaa ggcgctacct gttttaatct tgaagagttg ccggtaaaac actgggccat
     3901 gtcgatggcg caaaagcatg tgctggttgt cgaccacagt aaatttggca aggtgcgtcc
     3961 ggcgcgcatg ggtgacctga aacgctttga tattgtggtg agtgattgtt gcccggaaga
     4021 tgagtatgtg aagtacgcgc agacgcaccg tattaagttg atgtattaat gacgtataac
     4081 cggatgacgt ttcgcgccat ccggttatca gaagattaag agaaccagct gccgaaccac
     4141 tgatggaatt tcatcatcac gaaatcccac atccgaccaa agaatccgcc ctcttccaca
     4201 ttttccatca cgatcagcgg acgctgctca atggatttac cgttaagctg gaaatcaatg
     4261 gtcccgacaa cctgaccttt tttcagcggt gcggtaagct gcggttccgt taacgtgtag
     4321 ctcgccttca ggtttttcag ctgcccacgc ggtatggtca ctgagcccgc ttcgcctgcc
     4381 ccaagattca cttcgctctt atcaccaaac cagacgcgct gagtcacaaa ggtggcatca
     4441 ggtttaattg gcgtcacggt ttcaaagaag cggaaacccc aggtcaataa tttctcagac
     4501 tcattaaaac ggatacggtc agttttcgcc cccagcacta cggagattaa acgcatatcg
     4561 ccctgggtag ccgaagcaac cagattatat cccgcgcctg ccgtggttcc tgtcttcatg
     4621 ccatcaacat tcagattgct gctccacagc agacggttac ggttaggctg acgaattttg
     4681 ttgaaggtga actctttctc tttatgaatg gcgtactctt ccggcacatc gtggatcaat
     4741 gctttaccca gcaatgccat atcgcgcgcg gtactgaact gccccggcgc atccagaccg
     4801 tgcaccgtct ggaaggtagt gttggtcaga cccagttttt tggcataacc attcatcaaa
     4861 ccaatgaatg actcctggct cccggcaacg taatcggcca gcgcaataca ggcgtcatta
     4921 ccggactgga taatcacacc tttgttcaag tctgccaccg aaacctgatc gcccggtttg
     4981 aggaacatta ccgatgaacc acgcagtgcc ggatttcccg tcgcccaggc atctttaccg
     5041 accgtcacca tatcggtgag tttaatctta tcggccttaa gcgcctgccc aaccacatag
     5101 ctggtcatga ttttagtcag gctcgcggga tccagtttct catccgcgtt gccttctgcc
     5161 agcactttac cgctggcgta atccattaaa atccatgcac gcgcatccac gctcggcgct
     5221 tcaacggttt gttccgccgc gaatgccgtt ggggcaaaaa ggaataaaaa tgcagaaccc
     5281 gctgcaagac cacgaaggag agaggagtat tgcgtcataa atgccacccg agtatccatt
     5341 caaaaaaaat acgttatatc gccgtatgat aagttcggtg attaataacg cacacacaaa
     5401 cttaaagaaa ccgtaagttg gtaaagtttt taaagtttat gcaatatcct gccgcttcgc
     5461 tgcttctggt gtcatttttt tgccagctgt tgcttaaaag ttaactttac ttcaccatcg
     5521 gagtcctgtt cagcctgtgg ctgcctgtcg gaaaaggggt gagtatgatt acgctgtggg
     5581 gtcggaataa ttcaaccaac gtaaaaaaag tattgctgac gctcgaagaa ctggaactac
     5641 cttatgagca aattctcgcg ggccgtgagt ttgggataaa tcacgatgct gattttctgg
     5701 cgatgaaccc taacgggctg gtgccgttgt tgcgtgacga cgaaagtgat ctcattcttt
     5761 gggaatcaaa cgccattgtc cgctatctgg cggcacagta cgggcaaaaa cgtctgtgga
     5821 tcgactcacc ggcacgtcgt gcggaagcgg aaaaatggat ggactgggca aaccagacgc
     5881 tcagtaatgc tcatcgcggg atcctgatgg gattagtcag aacaccaccg gaagagcgcg
     5941 atcaggccgc cattgatgcc agttgcaaag agtgcgacgc cctgtttgcc ctgctcgatg
     6001 cggaactggc aaaagtaaaa tggttctctg gcgacgagtt tggtgtgggc gatatcgcta
     6061 tcgcaccgtt catctacaat ttgtttaacg ttggcctgac ctggacaccg cgtccaaatc
     6121 tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg gtgatgattc
     6181 ccgttagcta attgcgtggg ctaactttaa gtaattcccc actggactcg tcggtgagaa
     6241 cgtataaata accgtcgggt ccagtgcgaa catcacgaat tcgctgccct ctgtccgtta
     6301 aaatacggcc atcttctgtc actttgtcgc cgttgacgct catcacaatg acatctttat
     6361 ctttcagcgc gccaataaat aatttttgct gccactgggg gaatttatcg ctgttataga
     6421 aggccatgcc gctcacagcg ggcgaatctt tccagtaaaa aacaggttgc tcggtcccgg
     6481 cgacgatctc ccctttcgct tccggtatct taaagcctga atagttgatt ccccaggttg
     6541 ccagcggcca gccgtagttt ttgccttttt gcgggatatt aatttcatca ccaccgcgcg
     6601 ggccatgttc attcagccac agtgcattac tccacggatt cattgccatt ccttgcggat
     6661 tacgaatgcc ataagaccag atctcggcgc gcacaccgga ttcctttata aaaggattat
     6721 catccgggat ttcgccctgg tcggtcagac gcaccagttt gccctgtaat ttatccagat
     6781 cctgcgccgt cgggcgctga ttgttttcgc ccagagcaat aaaaagataa cctttaccgt
     6841 cgaataccag ccgcccgcca aaatggttgc cggtagacag ttttggcatc tggcggaaga
     6901 cggtgcggaa gtcggtcact tttgagagat catcacttaa gcggccataa cccacagcag
     6961 ttccggcttt gccatcatcg ccaacttcgg aataacttaa ccagatgcgg cgagactgag
     7021 caaaatcagg cgctaaaacc acgtccagca ggccgccctg cccgtgcgcc caaacgtccg
     7081 gaactccgga aagcggcgca gataatcctt ttcctgcttg ccagtgacgc aactcgccgc
     7141 cgcgcagagt gattaacata ccgtgattat cgggtaaaaa ggccagtgcc cagggatggt
     7201 cgagtttgtc ttgcagtact tcgacattta ccgttgcagg agccgcccag agagcggaag
     7261 aaagacaaat aaggggcaca aggaaaaagg attgtcgatg cataacgcct ccggattgac
     7321 tctggcttaa agcgtagtca gtggaggaga taacaaattc atttttacaa aaacttaaac
     7381 atgaaggggg agacgctttc tcccccttag ttttcaggcc ttctcaagca tggcgtgctt
     7441 ctgcaggctc tggatactca gcgttaagct catcagacaa ttttcaagct tatcggcgtt
     7501 gacggtaata acagtcgggc aatcatggtg cccactcatc aaacatactg cggctgtcgc
     7561 taatgcttct tcagcatgat gaagagcact ccactcttcc tgatccagat gaagattcaa
     7621 ccgcagcgat ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc gtagatgatt
     7681 gctttcgctg acggacatgt atccttttgc cttcctcagt tgcacgtatg cagcgaggtc
     7741 aacgcgtgcg tcgatcaacc ggttcagcag atcgattcgc tgtctgtcaa cgaacatact
     7801 tcgatcctcc tctttccagc cttgttgcaa ttaaatagta tggctatttt ttgagcaaaa
     7861 tgctgtccag gatcaattct tcacgccgaa ttaacaaaat ttacatttcc ttcgcctccg
     7921 ccagcgaatg agattgcgat acaaatttta tccagaactc tgtagaatcc ctgccctgac
     7981 ctttcctgta actgaatatt ttttaagcta tgagcaaagt aactccccag ccgaaaatcg
     8041 gctttgtttc ccttggctgt ccgaaaaacc ttgtcgattc agagcgtatt ctcaccgaac
     8101 tacgcactga aggttatgac gtggtaccga gctatgacga tgcggacatg gtgatcgtca
     8161 acacctgcgg ctttattgac agcgcggtac aagaatcact ggaagccatt ggtgaagcgt
     8221 tgaatgaaaa cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa gatcagatcc
     8281 gcgaagtcca cccgaaagtg ctggaaatca ccgggcctca tagctatgag caggttctgg
     8341 agcacgttca tcactacgtg ccaaaaccga aacacaaccc attcctgagc ctggtgccag
     8401 aacaaggtgt gaaactgacg ccgcgtcatt atgcctatct gaaaatttcg gaaggctgta
     8461 atcaccgctg caccttctgc attattccgt ctatgcgcgg cgacctggtg agccgtccga
     8521 ttggcgaagt gttaagtgaa gcgaaacgtc tggtagatgc gggcgttaaa gagattctgg
     8581 tgatctcgca ggatacttct gcctatggcg ttgatgttaa acatcgtact ggcttccaca
     8641 acggcgagcc ggtaaaaacc agcatggtca gcctgtgcga acagttatcg aaactgggga
     8701 tctggacacg tctgcactac gtttaccctt atccgcatgt ggacgacgtc atcccactga
     8761 tggcagaagg caaaatcctg ccgtatctgg acattccgtt gcagcacgcc agcccgcgca
     8821 ttctcaaact gatgaagcgt ccgggttctg tagatcgcca actggcgcgc atcaaacagt
     8881 ggcgcgaaat ctgcccggaa ctgaccctac gctcaacctt tattgtcggc ttccctggcg
     8941 agacagaaga agatttccag atgctactcg acttcctgaa agaagcgcgt ctggatcgcg
     9001 ttggctgctt taaatacagc ccggttgaag gtgcagacgc caatgccctg cctgaccagg
     9061 ttccggaaga agttaaagaa gaacgctgga accgtttcat gcagctgcag cagcagattt
     9121 ccgccgagcg cctgcaagag aaagtgggcc gtgaaattct ggtgattatc gacgaagtgg
     9181 acgaagaagg cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac ggcgcggttt
     9241 atctcaatgg tgaaaccaac gttaagccgg gtgatatcct gcgtgtgaaa gtcgagcacg
     9301 ccgatgagta cgatttgtgg ggtagccggg tttaattaat tcaggccggg caaaatcatt
     9361 tgtccggcct gcgaataatg attactgcca gctaatctga gccctgccgt ttttcttact
     9421 cttatacagc cgctcatccg ccagcatttt caagcgttcg acggtggtac attcatttga
     9481 atgcgctaca ccgatactgg tacttaaatt aatgcttccc acctggaaat gaacgatttc
     9541 ttttagaatc gatagcaact cttccagcgt tccacccgta taaatcaccg caaattcgtc
     9601 accgccgaag cggtagattt tacctttatc taccgggatc tgtgatttaa ggattttcac
     9661 cactttcatc aacacttcat cgcccagcaa atgaccgaag gtgtcgttaa tgtttttgaa
     9721 tttatcgacg tcaaacaggc acaaataacc gcctttttcc ggcagcgctt ttaaatcttc
     9781 gtcaaaggat tttctgccca ttgctttggt caggtcgtcc aggaacgccg agttatacat
     9841 actgtagagt tttttgacat cggcagccga aaagaaaagc ccgccatcca gtacgcgttc
     9901 aaaatggtta ttaatgatgg cttctttctc cacaatacca cgattaatca ctctgcgtac
     9961 cagatagagg caactggtac ccgatatcac tgccaccaat gcggcaaaga aaataatcaa
    10021 tgtacagacc cgcaaataaa catctttttt atacgcaaat acgtacaatc cggtcggcaa
    10081 ataggcgaag tgctccatcg cttccgaaac ggtgaagatg gcgtttttag cataatattc
    10141 atcaccggtc accggtttac cgttcttaaa tagagagaac atcaggttct cgctgtccat
    10201 cgccagcgat tttgacagta aagaaaaacg ctccagacca agcaaaaata tcagactacg
    10261 attgtgagtt accggataga ggtaatagac gaaggtttta ccgtcaaaag agataaaccc
    10321 tgtatttttg acatgtcgat acttcacaac ctgcataata tctgcacaat aatttttctc
    10381 gaactccttt acctgaatac attcgtcact ggaaatcaga taaggaccga catcgtacaa
    10441 ataagagggg gaaaccgagt cggcgtaagc tgtgcgcaaa ccaaccaaca gctgttcctc
    10501 cgtgggattt agcgtgttga taagatcaat tgccacgcta cgccgatatt cagcaatatt
    10561 tgccaccgca aaagcagtat caataacgga ctgtttcagg ctatacaccc gttcctttac
    10621 catttgagtg tagatcccgc aggcaactgc tgaaatcatg ataaaaatca gcagactgac
    10681 tgtaagtacg aacttattga ttctggacat acgtaaacta ctcttttact aattttccac
    10741 ttttatccca ggcggagaat ggcatgggtt tctggtacag atacccctga gcctgaacac
    10801 atcctaattt aattaattcg cgcgcaacgt cttcgctctc tacgccttcc gcaatcaccg
    10861 ggatccggtt atagcgtgcc agatcgaaaa taatgcgcac aatttttccc gcagtaccga
    10921 aaatatcgcc ggtaaattgc gagtccacct ttatgctgtc tggattaatt tcataaaaac
    10981 gtttcaggtt tgacaaaccg gtgccaaagt catcaatcga tatccgtacc ccttgtttac
    11041 gcagttgttc aatattgaga taaaaatgtt cattaattcg ctcgatcacg gtttcagtga
    11101 tctcaacaca gaggctgttc gccttcaggc cattttcttt catgatggtg gtaaaacgct
    11161 catgaaagtg cggttcgttt aactgccagg gcgaaacatt aatatgcagt aagaaatcat
    11221 ccttcagcga cgcacgctga ctaaaatgtc tgaattcacc cattgccgtt ttaataatct
    11281 gataacccag ctcattgata aacccgatat cttcagcaat ggtaatgaac ttcagcggtg
    11341 aaatgatccc ccgctccgca gaaacccaac ggcacaacgc ctccgcgccc agaatggcgc
    11401 gggtgttaat atcaacgatc ggttgataga cgaggaatag ttcgccaatt tccattgcat
    11461 ggcgaatatt acgcgcaatc cagatattct cttctatttc gttgagtttc gcgtcatcac
    11521 agaaaataaa ctcggagaac atatggtcac ggatactgga aatcgccagt ttcagttttt
    11581 ctgcgtaact ccaggcgttt tccccctgca aaggttcaca aatggcagac ttatggatgc
    11641 gcagattgcg gtagccaaag ctgctgtcaa aatcgtttac catcgctatt ttacgggtaa
    11701 atgtctgtac attttcccga cacagaagta aatagagatc ggcattgaat gtcacgatat
    11761 cgcagcattg cgcatatttc tcggcaattc gctgacacaa tttcctgacg attttattgc
    11821 gttcatcaac accaaacacc gcttccacgg cttccagacc acacagtttg atgatcccca
    11881 cagccagata ctgattattg ttggactcaa taatcgccat atttttaata aaaccgcttt
    11941 cctgatcgta agagatcagt gagagcaatt tatttgaccg ttcaatttcg gcatctttca
    12001 tttccgcgat cgaatttttg atgctgataa tctctttcgc atcgccttcg cgaattttca
    12061 gagcctcgat ctgatccgtt gagtcgggca aaaacgccag cgtattaacc agctcggtca
    12121 tttcagaaaa ttgccgacgg aacacacgag tggtcatgaa atataccacc agcaacacga
    12181 taataatgaa cgcaaacggt agataaaaaa gtttatctga tgcgttcaat aaattaccgc
    12241 caggataata aaaatagatt tcgaacaatg cattatttgg cactgctgat gtctttgcaa
    12301 tatactccgt atcgacagaa tcaataatat tataattacc ttcgctatta taaataatca
    12361 gtttttcacg agcgagttga ttcttggcaa tgatctcatg accttgtttc aaaaatagaa
    12421 cttcatcatt agcgctgatt tgatcaagat actcgttcag ataccgccag tttaaatcga
    12481 catgaatttt atctaccgct aacaaatcgt gatctttgcc gttgatacgt agcgtaaaag
    12541 agatacattt tttacccgga cgatcaaagc aattgtatgc cggattccag ctgttgttag
    12601 agtcatctga agccaccgac cacggtcgtt tcgatgggtt aaaatcggca aaaataaccc
    12661 gccctgactc gtcgacaagc ggtccattta cttgttcctg atgataaaca acgattttat
    12721 cattcgccct ccgtgaaaag agataatatt tattatcatc aagtaccagt cccaccgagt
    12781 ctaaaaatgg catcatttcc attttcagaa tcacggcgcg t
//
LOCUS       DAZGLJ010000130        12481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000130 DAZGLJ010000000
VERSION     DAZGLJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.130"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..473
                     /locus_tag="ACHK49_003215"
     CDS             <1..473
                     /locus_tag="ACHK49_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFO9263824.1"
                     /translation="SSVGSRMGLGRFSGFFRSAGGLAGRLGGGALWAGAMAAPVLLDG
                     SASATDKGEAVGSLAGSIAGGALGAAAGPVGIAIGSTVGSYLGNYLGGWLTEAWQKLR
                     GSDDESSGQAVQKASARVELVAPEGWQARSIDIDDTSGHGLDVNVWNGGNYGLW"
     gene            460..1809
                     /locus_tag="ACHK49_003216"
     CDS             460..1809
                     /locus_tag="ACHK49_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HFO9263825.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLAEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGKVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSAFSQGMPQLYRTLSSLRYVLDEQD
                     NPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRTW
                     PVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQL
                     PGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIVDPLFIPGGHSVEVISEQQG"
     gene            1793..2917
                     /locus_tag="ACHK49_003217"
     CDS             1793..2917
                     /locus_tag="ACHK49_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO9263826.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRHSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSVR
                     LTATVRHWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            2907..3521
                     /locus_tag="ACHK49_003218"
     CDS             2907..3521
                     /locus_tag="ACHK49_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO9263827.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEAHFTGNVTVDKNLHVKGNV
                     SIDGTGRSQGTFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            3514..3951
                     /locus_tag="ACHK49_003219"
     CDS             3514..3951
                     /locus_tag="ACHK49_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO9263828.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPVGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHARLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            3951..5033
                     /locus_tag="ACHK49_003220"
     CDS             3951..5033
                     /locus_tag="ACHK49_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO9263829.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLKSVMAGDTELLTVGEITWL"
     gene            5024..5584
                     /locus_tag="ACHK49_003221"
     CDS             5024..5584
                     /locus_tag="ACHK49_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFO9263830.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEENI"
     gene            5584..6495
                     /locus_tag="ACHK49_003222"
     CDS             5584..6495
                     /locus_tag="ACHK49_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9263831.1"
                     /translation="MFHVDNNSGVANMPALAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGALATKNSLGPGDVNALAKDQNLADLENAGTARNNLDVYSKSEGDNRYLRR
                     EQNGADIPDKGAFIDNVGLRETVNKAADALPSGGTAVAANRLATPRNINGVPFDGTLD
                     INITSGMTQSTADGRYVQNVQLGAQSYHSPGGNEMSWNYSAPSGCMLSGINVQETGSR
                     SADNIGGVYYRPVQIYINNAWRTVSSV"
     gene            complement(6530..7051)
                     /locus_tag="ACHK49_003223"
     CDS             complement(6530..7051)
                     /locus_tag="ACHK49_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO9263832.1"
                     /translation="MELINVKRYYPEHKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     CISPVTGIICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKT
                     VAEKRRSSLLQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMHFNDIASE
                     GHYQAIPWPEKPE"
     gene            complement(7131..7334)
                     /locus_tag="ACHK49_003224"
     CDS             complement(7131..7334)
                     /locus_tag="ACHK49_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9263833.1"
                     /translation="MADSRYVQSIRRGSRSTIGMQYNIFEVPDGCVLTGLDVAGDGNA
                     TVTAYYRPVQFLIDGSWKTASSA"
     gene            7363..7542
                     /locus_tag="ACHK49_003225"
     CDS             7363..7542
                     /locus_tag="ACHK49_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263834.1"
                     /translation="MSCVPSNGTPLIFLGVASLFAATAAPFDGRAFAALFTVSRKPRF
                     QGCRISALKGLSTSF"
     gene            7556..8116
                     /locus_tag="ACHK49_003226"
     CDS             7556..8116
                     /locus_tag="ACHK49_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO9263835.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYYPASVSEK"
     gene            8216..10255
                     /locus_tag="ACHK49_003227"
     CDS             8216..10255
                     /locus_tag="ACHK49_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO9263836.1"
                     /translation="MTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMTFAD
                     CLISFNTRDCLWVRPVSGDPSVEITGAGIGAVIPLSADVAGTAEPSDWDNAETHTRPS
                     GNETASSSPSWYYVVVLAGQSNGMAYGEGLPLPETYDRPEPRIMQLARRSTVTPGGKA
                     CQYNDIILADHCLHDVQDMSGKNHPKADVAKGQYGTVGQGLHIAKKLLPFIPADAGIL
                     LVPCCRGGSAFTAGADGTYSDSTGASEDSARWGVDKPLYKDLISRTKAALAKNPKNRL
                     LAVVWMQGEFDIDAKPTEHSALFLAMVEKFRADLAEQAEQCTGGSAAGVPWICGDTTY
                     FWKQKNEPAYQAIYGGYKNKTDKNIHFVPLMTDENDANVPTNNPAEDPDIESIGYYGS
                     TWRNSAATWTSADRASHFSSWARRGIISDRLATAVLTHAGRTTVKADVPSSETEAPVP
                     SPSETEAVTTTLLSYRVSESEGNLKAQGWEPAGGKAEIISDAGGTGGKAMKLTKETGK
                     SSWYLDHDAGTGAELLKNGGLISCRFKVPGDLVANQYVMGLYWPVSSLPQGVTLTGDA
                     GNNLLASFYIQTDAKDLNVMYHNAKVATNNQKLGTFGAFDNEWHTLAFRFAGNNSLQV
                     IPVIDGQDGAAFTLTQSPVGTFPVDKLRVTDITKNATYPVLIDSIVVEVKKAVTE"
     gene            10402..10584
                     /locus_tag="ACHK49_003228"
     CDS             10402..10584
                     /locus_tag="ACHK49_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO9263837.1"
                     /translation="MTFVHTMLLYFCAVVSALYLVSGGYKVIRNYIRRKIDDAAAEKL
                     SKTAQAPSSPNDPTPL"
     gene            10620..10865
                     /locus_tag="ACHK49_003229"
     CDS             10620..10865
                     /locus_tag="ACHK49_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO9263838.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGRSPAENSTENDPQPVEQPEPVQPGTDGTMM"
     gene            complement(10904..11368)
                     /locus_tag="ACHK49_003230"
     CDS             complement(10904..11368)
                     /locus_tag="ACHK49_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263839.1"
                     /translation="MIQGGDYLLKENEELGGENDHKMIILLTFYTVKVGSRVYVVCFK
                     STGYYIKVKPSIAGTMLGQSVKTCINALLRARGVSYDIPRSPVKQRCIKRRSLSWMKE
                     PVCPACKTPLQLIYRRSRSQNGKTYKPKNASCFRMLICPACSPRRENTQNVH"
     gene            11483..11683
                     /locus_tag="ACHK49_003231"
     CDS             11483..11683
                     /locus_tag="ACHK49_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9263840.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSPSATEHP
                     TYTRKSCRGEQKASHIPNHQHA"
     gene            11637..12374
                     /locus_tag="ACHK49_003232"
     CDS             11637..12374
                     /locus_tag="ACHK49_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFO9263841.1"
                     /translation="MGNRKQVTSRIISTPELIRYNDNIVGYGSRELRVETISCWLARL
                     VIVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNSGARVVTGTQNREYMELN
                     RLWLHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHESIFYELDGEWYHEICRNAIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLNKRAR
                     KRLNTKLFKVQPYPKPQTVKTGLKESE"
BASE COUNT         3060 a         3009 c         3463 g         2949 t
ORIGIN      
        1 tttcgtctgt cggtagccgc atggggctgg ggcgtttctc cggctttttc cgcagtgccg
       61 gtggactggc cggccggctg ggtggcggtg ccctgtgggc tggcgcgatg gcagccccgg
      121 ttctgctgga tggcagcgcc agtgccacag acaagggaga agcggtcggc tctcttgcag
      181 gcagtattgc cggtggtgcg ctgggggcgg cagccggacc tgtggggatt gccattggtt
      241 ctacggtggg cagctatctc ggtaactatc tggggggatg gctgactgaa gcctggcaga
      301 aattacgggg cagtgatgat gaaagcagtg gacaggcggt acaaaaagcc tctgcccgtg
      361 tggagctggt tgccccggaa gggtggcagg ctcgcagtat tgatattgat gacacgtccg
      421 gtcacggact ggatgtgaat gtctggaacg gagggaatta tggcctctgg tgacggtcgt
      481 ggtgcgttcc gtggtgtgcc gttcctcgtc tggcgtgagc agcgcgaacg tggcgggcga
      541 aacattgtcc gtcgggaata tccgttacgg gaaaccggtg gtgcagacga tctgggacct
      601 aaactggctg aatttacgtt cagtgtactt gtgatggggg atgatgttca gacacagcga
      661 aatcgtctgc gtgatgccct tcgtgctccc ggtgccgggg agctgcttca cccggattac
      721 ggcacgttaa atgtgctgat aaacagcttt gaaagtcgtt ataacgctgc tgaacagggc
      781 gtggttgagt tcaccattaa tgtcacgccg gtaagtgatg ataccgcacc tgctgtcaca
      841 caggacacgg ccgccattct ggagcagaag agtaccaccg cgctcgggaa ggtttttgaa
      901 accctggaag cgggctggac ggtgatttct gacggaatgc acgatgtcca ggcaatgacc
      961 gaaaccatca gcgataaggt gtccgccctg gaaaatgccg tttccggcat ggggattgta
     1021 caggatatca gtgcctttac tgccacgttc acggcgctga aagggaatgc cacggcatta
     1081 ctgacagcac cttcccgcat ggcctcatct tttgcggggc ttttcagtgc cctgatcacc
     1141 ctgccatcgc tgccgtcact gtcttcagga ggcctgaata cccggcccgg tggcagtatc
     1201 ggccggacga catccgcctt ctctcaggga atgccgcaac tgtacaggac attatcttcc
     1261 ctgcgttacg tgctggatga gcaggataat ccgcagactc tcatcggtct gacaccggca
     1321 gcacaaaaga atattcgtct gatacgggcg gtaatgcaga gtgctgccgt ggtggcccag
     1381 gcacagaccg tgggaaaact gctggatcag gttctcagtc aggaaaccct gccggacagt
     1441 gacgcagccc accgtacctg gcctgtctgg ctggaaagtt cagttgatct tcagcgcatt
     1501 aaccgtgact taagcgaagc gctcgaacgg caggtgatga cactgtccgg gcaggggtat
     1561 accgccacgg cgctgacgct tcgtgatgcc agactggcac tgacagagga tctgaataca
     1621 cggggagttc agctacccgg tgcaacagta gtgactgtac gtaccaccga gcctgcactg
     1681 gtgaccctgt accgtgccac cgggaacagt accggctggc aacgttttgt gcgccgtaac
     1741 ggtattgttg atccgctgtt cattcccgga ggccattcag tggaggtgat tagtgagcag
     1801 cagggttgaa ctgtatctgg gcggtgagat tttttccggc tggctgacgg tgagtgttcg
     1861 tcgctctctt gaacatctgg cgggctcctt tgaactgggg gtaatgatgc ccggtgtacg
     1921 ccttccgtca tccgtccgtg ccggtcagtc tctggaattg cgcattgacg gtcagcctgt
     1981 gatcactggc tggctggatc aggtccggca gcgcatcagc gccacgcgtt ttcagatcac
     2041 gctcagcgga cgggataaaa ccggtgacct ggtggactgt tcagccattc atccgggcag
     2101 ccagtggagg aaccgcacgc tggagcacat tgcttcagat ttgtgtgctc cgttcggggt
     2161 cacggtgcgc tggcaggtaa atgatgcaac ggcagcccgg cccttttcca ccttcacact
     2221 ggaaaactca gaaaccgtgg cagatgcgct gacacgggcc gcgagacaca gaggcgtcct
     2281 ggtaaccagt aatgccgccg gtgaactggt tttcactcag gccggcagtc agcgtggcga
     2341 cacgctgacg ctgggcgaaa atctgctgga tttggatcac aacgtggatc accgtctgcg
     2401 ccacagtgaa taccgtgtac gggggcacgg gcgtggtggt ggtcatgccg gggatgcact
     2461 gacagccgga acgctggccg cacccgttgg tacggtgaca gacagtgcca tccaccgtta
     2521 cagaccgaaa attgtgctgg cggatcatgc tgttgatgca gacggtgcac gccagagggc
     2581 tgtccgggaa atgcgccggg cggttgcccg ctctgtgcgc ctgacagcca ccgtgcggca
     2641 ctggtttcgg gagaacggcc agttgtggga tatcaacctg ctgacggctg tcacggctcc
     2701 ccgcaccgga gtggaagagc gtgatcttct tgtctgtcag gtggagtttt cgctggatgc
     2761 aaatcacggc gaaaccaccc gtctgattct ggcaccccgt gacggcttta ttgttccggc
     2821 agagccggga aacagcggaa gcggaaatgc gggtgacgtg gacgccttcg tgcgggcaca
     2881 gatgaaaaaa caggggatta aattcaatga tgaatgacga agtcatcagc cgccttctgg
     2941 cccccgtgat gcggggtgtt cgtctgctgt tcgggcgtgg tgtactgacc ggcacaacgg
     3001 acacgctgaa aatccagaat gtgcaaatca ccggtatgga tggtgaaacc tttgatgacg
     3061 ttgaacgccc ccagcagtac gggcaaatca gcgtccccct gcctggtgcg gaagtttttc
     3121 tggcctgtgc tggcggacaa cgggatcagg ccgtggtgct tgtggtggaa gaccgccgca
     3181 gtcgcccgac cggacttacc gccggagata ccggcgtgta tcaccatgag gggcaccgta
     3241 tacgtctgac aaagaacggc cggatcattg tgacctgtaa gacgctggag atttacgccg
     3301 atgaaggtgt tcaggtggat acaccggagg ctcactttac cggtaatgtc acagtggata
     3361 agaacctgca tgtcaaaggc aatgtgtcca ttgacggcac cggaagatca caggggacgt
     3421 tcacgatgtc cgaagcggtt attgccggga tcacctattc aggtcatgtg catcacgata
     3481 acggtgaagg cagcaaaacg ggaggaccag agaatggctg atattgctgt cgtctgggat
     3541 cagggttgcg gttccctgca actgaacggc gcagatcttc tgacggataa cagcctgctg
     3601 actgcggtca ttatttcact gtttacggac aggcgggcgc tggattccga tgaaatccct
     3661 gacggcaccc gtgaccgtcg gggatggtgg ggagacagtt tccgggagcg ccccgttggc
     3721 tcccgtctct ggctgttaag ccgtgaaaag acgctgtcct ccgtggtcag ccgtgcacag
     3781 gcctatgctg atgaagcgct ggcgtggctg cataaaagcg gtgctgccac atccgtggta
     3841 tgtcatgcca tgcgtgtggg gcatgctcgc ctttcgcttt ccgtgaaaat caccctgccg
     3901 gacggaagca gacatccgat gattttttat gctgatatga agggggaatg atgccttatc
     3961 agcccttacc actggcgcaa ctgatcacac agacacaaca ggatatcagc cagcgcctgc
     4021 ccggttcgca gccgggcgtg aatgaaacca ccctgaatgc cattgcgtat gcactggcgg
     4081 gtctgtcagc acaggagcat gaacatctgg cctggatctc ccggcagata attccgacag
     4141 aagctgatga agccgaactc ctgaaacact gcgcattctg gggtgtcatc cgtaaaccgg
     4201 cttcccgcgc tgacggaccg gtacaactga tgctgaccac ggatgcaggg atcacggaag
     4261 gcgtactcct tcagcgaagc gatggtgttg tgtaccgcat caccggctct gcgaccggaa
     4321 aagccggaac actgaatgtt aatgtggagg cggaaagtgc ggggcgcgct ggaaataccc
     4381 cgaccggaac ccgcctgtcc tttatcacgc cacaggcggg catcaaccag acagccacgg
     4441 tcaccggcac gggactcacc ggtggtgcgg atgtggaaac ggtgccggag ttgctgtcca
     4501 ggctggtatt ccgggtacag aacccgccat cagggggaac acagtatgat tttgaacgct
     4561 gggcacggga agtaccgggc gtgacgcggg catggtgtaa gcctgaatgg cctgaggcgg
     4621 gtagtgttgg tgtgactttt gttcaggata ataaccctga cattttcccc ggagaaggtg
     4681 atgtgaagcg ggtggcggat tatatccgca gtcatgatga tccggcgacg ggccagcccg
     4741 ttggtcagcc acttgggccg acaatcagcg tgtttaagct gaccaataag ccggtggcgt
     4801 ttgagatcag gattgtaccc aaaacgccgg aaaatcaggc tgccgtaaaa caggcattaa
     4861 cggacctgct ttataacgaa tcgcggccgg gtggacttgt attgccttca tcattctggc
     4921 gggctgttgc aggggtgaaa ggactggagg attttgaagt tcgcagcccg ctgaagtccg
     4981 tgatggccgg agatacagag ttgctgaccg tgggggaaat cacatggctg taaccctgac
     5041 cccgcatcag cgtgccctgt tgcagttgct gcctgacggg ctggcatggg ataagcggcc
     5101 gtcatccgtt cttgcggctt tgtgcctggg cctcagtcat tccacggagc gtgtttcctg
     5161 gaccggtaac cagatgctgg cagaacgttt tcctgattca tcccgtctgc tgctggaaga
     5221 ctgggagcgt tatctggggt taccggaatg tgatatgacc ggcgcaacca ttcaggagcg
     5281 tcagcgttat gccgggaata aataccggat gaaaccctct cttaaccgtg aattttatat
     5341 ccggtttgcg gcagagtttg gttatgaaat agatattcag ccatcaccgg attcacagtg
     5401 ggtcagtatt gtcacgatta acagtgaaac cggctaccgg aatatgaatg tgctggatga
     5461 tattctcacg ccgctgcgta tttatgaagg cggtgcgctg gaatgtattc tgaaccgtta
     5521 taagcctgca tggcagacgt ttatttacgt atatgcaaac agtcacgaag aggagaatat
     5581 ttaatgtttc atgttgataa taattccggc gtggcgaata tgcctgcgct ggcaccggcg
     5641 cagagtaata ccaccacctg gtttaccgaa ggtgacggac aaaaaggtat cagctggatt
     5701 ggtcaggact ggctgaatat tctccaggcc gaactgctga atattctggc tgaagccagt
     5761 attcagccgg ataaggcgca gttaaaccag cttacgctgt ccattaaagc cattatcgct
     5821 gcgaatgcct tttcccggaa aaacaacctg aaagaaattg ctgatgccgg tgcggaagcc
     5881 cagcgtcttg cccgtggtta tcttggtctt ggggcgcttg ccacaaaaaa cagtcttggt
     5941 cccggtgacg ttaatgctct ggcgaaggat cagaaccttg ccgacctgga gaatgcggga
     6001 accgcccgta ataatctgga tgtttacagc aaaagcgaag gtgataaccg ttacctgcgc
     6061 agggagcaga acggcgcaga cattccggat aaaggcgctt ttatcgataa cgttggttta
     6121 cgggaaacgg taaataaggc tgctgatgcc ctgccatcgg gtggaaccgc cgtggcagca
     6181 aacagactcg ccaccccaag aaatattaat ggtgttcctt ttgacggaac gctggatatc
     6241 aacatcacgt caggaatgac gcagtcaacc gcagatggcc ggtatgtaca gaatgttcag
     6301 cttggagcac agagctatca ttcacccgga ggcaatgaaa tgtcatggaa ttacagcgca
     6361 ccttccggtt gtatgctttc cggaattaac gtgcaggaaa ccggctcccg gtctgcggac
     6421 aatatcggtg gggtctatta tcgcccggtt cagatttata ttaataatgc ctggagaacg
     6481 gtaagttcag tgtaaaccac agaaaagggt gctgaatgca ccctctgaat tattccggtt
     6541 tttccggcca gggaatcgcc tggtaatgcc cctcactggc aatatcatta aagtgcattt
     6601 ctttaagctg tcggatatac gccatccagc gcgtcagctg taatttattt tcatcgctaa
     6661 tcatgtccag ttttaattcc gttttccagt catccacagt ttcgttggct tcctggagca
     6721 gcgatgagcg gcgcttttca gcaaccgtct tccagtttac cggcaccttt gatatcaggc
     6781 catcagaaaa ctgccagtta ccgtcaatat tcacaccttc cggcagttcg tccacttcaa
     6841 caacagtaaa acctgccgga tataaagcag agacatcttc agcaacagag cagataatac
     6901 ccgttacagg cgaaatacag agtttgtatt tctttgtgaa aagcgggata gattcataaa
     6961 aatcccttcc gtcctcactc tggaaatact gaacatcctc accatagggt ttgtgttccg
     7021 ggtaatatcg ttttacgtta ataagctcca ttatttcacc ttgtttttgt tgaaaagaca
     7081 aaatcatcat atatttattc agataatctg ttttttgaac tactgcactt ttatgcgctg
     7141 gaagctgttt tccacgaacc atctatcaga aactgaacag gtcgataata tgcggttacc
     7201 gttgcattac catcaccagc gacatcaagc cctgtaagta cgcatccgtc aggaacctca
     7261 aaaatattat attgcattcc aatcgttgac cttgaacctc ttcgaatgct ttgaacataa
     7321 cgtgagtcag ccacagactg cgtcatcccc gatgtaatat ttatatcctg cgttccgtca
     7381 aatgggacac cattaatatt tcttggagtg gcgagtttat tcgctgcaac agccgctccg
     7441 ttcgatggca gggcattcgc ggccctgttt acagtatccc gtaaaccgag gtttcaggga
     7501 tgccgtatat ctgctttaaa aggcctttcc acctcttttt aaggaaggta aatacatgct
     7561 gattggctat atacgcgtat caacaaatga ccagaacaca ggtttacaaa gaaatgcgct
     7621 ggaatgtgca ggatgtgagc agatttttga agataaaatg agtggtaaaa cgacacagcg
     7681 gccgggactg aagcgggcac tgaagaaact aaaacccggt gacacactga tggtctggaa
     7741 actggacagg ctggggcgca gtatgcgcca ccttgttgcg ctgacagatg aattacagca
     7801 acaggggata aattttcgta gtcttactga caacattgat acgtcaaccc ccatggggcg
     7861 cttctttttt cacataatgg gtgcgctggc agaaatggag cgtgaattaa ttgtcgaacg
     7921 gacacgggct ggtcttgcag ctgcccgtga aaggggacga accggcggca gaccccgtcg
     7981 tctcacgcag gaacaatacg agcaggtcgg gcgtttactg gccgggggtg tcagtcgaag
     8041 acaggtggca cttatttttg atatcggcct ttcaaccctt tatcgttatt atcctgcgag
     8101 cgtttcggaa aaataagacg ctccctttgt tgggccttta ctgaattaac gcaaaatggc
     8161 gtttatcggt ctgtggatat gataattcag tttattcagg aacaggctga ttattatgac
     8221 gtccaaatgg gttcaactat catcaatgcc cggaaacttt actgttaaag tttccggtgg
     8281 tacagcggca tttcttgagg ctccctttcc tccggccgaa acaaaaggag gaatgacatt
     8341 tgctgactgt ctcatcagtt ttaatacacg ggattgcctg tgggtaaggc cagtatccgg
     8401 tgatccgagt gtggaaatta ccggagcggg tattggtgcg gtcattccgt taagtgctga
     8461 tgttgccggt actgctgaac cgtcagactg ggataatgct gaaacacata cccgtccgtc
     8521 aggaaatgaa actgcttcca gctccccttc ctggtattac gtggtggttc ttgctggtca
     8581 gtcaaacggt atggcttacg gtgaaggtct gccactgccg gagacatatg accgccccga
     8641 accgcgtatt atgcagttag cccgtcgcag tacagtcacg ccgggaggta aagcctgtca
     8701 gtataacgac atcattctgg ccgaccactg tctgcatgat gtgcaggata tgagtggaaa
     8761 aaatcacccg aaagcggatg tggctaaagg gcaatatggc accgtgggtc aggggcttca
     8821 cattgccaaa aaactgctgc cgtttattcc ggcagatgcg gggattctgc tggttccctg
     8881 ctgccgtggt ggttctgcat tcacggcggg tgctgacggc acgtacagtg acagcacggg
     8941 tgcttcagag gactcagccc gctggggtgt ggataagccg ctgtataagg acttaatcag
     9001 ccggacaaaa gcggcactgg cgaagaaccc gaaaaaccgc ctgcttgctg tggtgtggat
     9061 gcagggcgag tttgatattg atgcgaagcc gacggagcat tccgcgctgt ttctggcgat
     9121 ggtggaaaaa ttccgcgcag acctggctga acaggcggaa cagtgtaccg gtggcagtgc
     9181 tgctggcgtt ccgtggattt gtggcgatac cacgtatttc tggaaacaga aaaatgaacc
     9241 ggcataccag gcaatctatg gcggctacaa aaacaaaaca gataaaaaca ttcatttcgt
     9301 tccattaatg acagatgaaa atgacgcgaa tgtacccacc aacaatccgg cagaagaccc
     9361 ggatattgaa tccattggtt attacggctc aacgtggcgt aacagtgccg ccacctggac
     9421 atctgcggac cgggccagcc atttcagttc atgggcacgt cgtgggatta tttccgaccg
     9481 tctggccacg gcagttctga ctcatgcggg gcgaaccaca gtaaaagccg atgttccatc
     9541 ttctgaaact gaagcaccag tgccgtcacc ttcagaaact gaagcagtaa ccacaacact
     9601 gctgtcttat cgtgtcagtg agtcagaagg aaatctgaaa gcccagggct gggagccggc
     9661 tggcggtaaa gctgaaatta tcagtgatgc aggaggcaca ggtggtaagg caatgaaact
     9721 gaccaaggaa acaggtaaat catcttggta tctggatcat gatgccggta ctggtgctga
     9781 actgctgaaa aatggtggtt taatcagttg tcgttttaaa gtacctggcg acctggtggc
     9841 aaaccagtat gtcatggggc tttactggcc ggtttcctct ctgccgcagg gtgtcaccct
     9901 gacaggggat gcagggaata atctgctggc atcgttctac atccagacag acgcaaaaga
     9961 cctgaatgtg atgtaccaca atgcaaaagt ggcaacaaat aaccagaaac tgggaacctt
    10021 tggtgcattt gataatgaat ggcatacgct ggccttccgt tttgcaggaa ataacagcct
    10081 tcaggttatt ccggttattg atggtcagga tggtgccgcg tttaccctga cgcagtcacc
    10141 agtaggaact ttcccggtgg acaaactccg cgtaacggat atcacaaaaa atgcaactta
    10201 cccggtgctg attgacagca ttgtggtgga agtgaaaaaa gcggtaacag aataaaaaaa
    10261 tcccgccggt catgatatgg ctggcgggag ctacctaacc cataaaggaa aataaaaata
    10321 ctcccgaagg agtggttatc agtcgcttcg atgtttttac ccgataacga attatgtagt
    10381 caatatcaaa ggtaaggtta tatgacattc gttcatacaa tgctgcttta tttctgcgct
    10441 gtggtcagtg cgttatatct ggtgagcggt ggttataagg tcatcagaaa ttatattcgc
    10501 cgtaaaattg atgatgcggc agctgaaaaa ctcagtaaaa cagcacaggc accgtcatcc
    10561 ccaaacgacc cgaccccgct ctgataacgg ggcaatatac ctgcaaaagg agaatattca
    10621 tgccagagat taaaggcacg gttactgaag agcttgtcaa acaggcactt tattctgaag
    10681 aagtgaaccg cgtgctgaag gcgcaggttc gcaaggattt tgaggcacaa atcgacgcat
    10741 atgttgatga agtgctggcc cgaatggttg gccgttctcc ggctgaaaac agcacggaaa
    10801 atgatcctca acccgtagag caaccagagc cggttcagcc cggaactgac ggtactatga
    10861 tgtaacaata ccgaccggca ggtttccctg ccgggtgagt ttgttaatgc acgttttgtg
    10921 tattttccct cctgggagaa caggcaggac atatcagcat cctgaaacag gaagcatttt
    10981 tgggtttgta tgtcttgcca ttttgagatc gagaacggcg atagatgagc tgtaagggag
    11041 tcttacaggc agggcaaaca ggctctttca tccatgataa agatctcctt tttatgcatc
    11101 tctgctttac aggagaccgg ggaatatcat aggatacgcc acgagctcga agtaaagcat
    11161 tgatgcaagt ttttacagat tgacccagca tagttccagc tatgctgggt ttcactttta
    11221 tgtagtatcc tgttgatttg aaacatacta catatacgcg tgatcctacc ttcactgtat
    11281 aaaaagtcaa taaaatgatc attttatgat cgttttcacc acctaactct tcgttttctt
    11341 taagtaaata atcacctccc tgaatcattg ttgatgactt ttcaatctat ttgttgtatc
    11401 aattaaacaa atcgaatcga tcgtttttgt cgatcgattg catgagaaag gcatcaacaa
    11461 caaagcagga ggttatgcgg tcatgtatcg aaacatacgg tgccgacact gcaataagct
    11521 actggcacgg gccagtttca gttatctgga agtaaaatgt ccgcgctgta aaaccctcaa
    11581 tcaaatcaca tctccgagcg ccacagagca ccccacatac acaaggaaat cctgtcgtgg
    11641 ggaacagaaa gcaagtcaca tcccgaatca tcagcacgcc tgaacttatt cgctataacg
    11701 acaatatcgt tggttatggc tcccgtgagt tgcgggttga gacaataagt tgctggctgg
    11761 ccagactggt catcgtcaat aaacattaca gccaccgttt cgtaaataac tcctaccttc
    11821 atctgggcat attttcagaa cgggaacttg ttggcgtaat gcaatggggt tacgccctta
    11881 acccaaacag tggtgcacgc gtcgtaacgg gtacgcagaa ccgagaatat atggagctta
    11941 accggctatg gctgcatgac tgtatgccga gaaactccga atcaagggca atcagttacg
    12001 cgctcaagct aatcaggcag ctttatccgc aggtacagtg ggttcagtcg tttgcagatg
    12061 agcgttgtgg ctgtcttggt gtcgtgtatc aggcaagcaa ttttgattat gtaggcagcc
    12121 atgaatcaat attctatgaa cttgatggcg agtggtatca cgagatttgc aggaatgcca
    12181 ttaaacgagg cggacaacga ggtgaacatc tgagggctaa tattgacaga gccagtgttc
    12241 ataagttccg acagtttcgt tacatccggt ttctgaacaa gagggccaga aaacgcctca
    12301 acacaaagct attcaaagtc cagccttacc caaaaccaca aacagttaaa actggtttga
    12361 aagagagtga atgagcgctt aaaaactcat tctaaaactc aaaagaaaag cgccgattta
    12421 gtatcaaata agttgcgtat cggcgttttt cagcggtatc aaatgaaatg caaatcggta
    12481 t
//
LOCUS       DAZGLJ010000131        12392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000131 DAZGLJ010000000
VERSION     DAZGLJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.131"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(113..394)
                     /gene="yehE"
                     /locus_tag="ACHK49_003233"
     CDS             complement(113..394)
                     /gene="yehE"
                     /locus_tag="ACHK49_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO9263842.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(657..1766)
                     /gene="apbC"
                     /locus_tag="ACHK49_003234"
     CDS             complement(657..1766)
                     /gene="apbC"
                     /locus_tag="ACHK49_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO9263843.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1898..3931
                     /gene="metG"
                     /locus_tag="ACHK49_003235"
     CDS             1898..3931
                     /gene="metG"
                     /locus_tag="ACHK49_003235"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO9263844.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4072..7866
                     /locus_tag="ACHK49_003236"
                     /pseudo
     CDS             4072..7866
                     /locus_tag="ACHK49_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7876..11508
                     /gene="yehI"
                     /locus_tag="ACHK49_003237"
     CDS             7876..11508
                     /gene="yehI"
                     /locus_tag="ACHK49_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO9263845.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVIARQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFNQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVHRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11570..11887
                     /gene="yehK"
                     /locus_tag="ACHK49_003238"
     CDS             11570..11887
                     /gene="yehK"
                     /locus_tag="ACHK49_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO9263846.1"
                     /translation="MIVQKELVAIYDYEIPVPENPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDTDPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
BASE COUNT         3241 a         3149 c         3043 g         2959 t
ORIGIN      
        1 atcaattaat gtagatttat gtaaatggta ttgttgatat caataaaaaa gccgaaatta
       61 tttatataaa tgatttcggc tttctaaatg caattaaaat caggttgcca aattaatcgt
      121 agcgatttaa cacgattcga cgtttgctat cgctacctgc aacaaccact tctgtcgtta
      181 cacctttgac gggcgaagaa aacagatgac gggtgtcaac attatacgtt aactgaccac
      241 aatgctgttg aggttgacca ttaaccatag ccatactgca tgttggggga ctgatcctgc
      301 cattaatcgc taatgtggca gtctctgaag agaatgcggg agaagccaat ccataggcaa
      361 gaaaaataat tcctgacagc caatacttat tcattgaacg ttatccctgt agtaaaggtt
      421 atgcctggat agaatgagtg cataacaaac tatagctgta catccactac acagccacga
      481 aggatgataa tgaagcattg cctgtatgat caatcgactt tgtagagttt cggacgaagg
      541 tccgcagaat attcgcagta ttaaataagt cttcattaat gcggcaaaaa ctaatacacc
      601 gcatcaatgt aacatctctg cggtgtatga agaataacca gaatggttaa taggcgttag
      661 accgcgcgga aggaaatctc gcctggaatg acttcaccct gccagtagag ctgagctgca
      721 acgcggtcag ccagctggcg gtagatagcg gtaaattcgc tctccggacg gctgataacc
      781 gttggcgtgc ctttatcgag atcctcacgt aaggagatgt gtaacggcat ctgccccagc
      841 agctgtgtgt ggtatttttc agccagtttc tcagcaccac cggtaccgaa aatcggttca
      901 tggtgaccac agttactgca aatatgcaca ctcatgttct cgacaatacc aagcaccgga
      961 acctcgactt tctcgaacat cacaatgcct tttttcgcat cgatcaacgc gatgtcctgc
     1021 ggggtggtga ccacgactgc accagttact ggaatgtttt gcgccagcgt caattgaatg
     1081 tcaccggtgc ctggcggcat atcaagcacc agatagtcga gatccggcca caaggtttcc
     1141 tgcaacatct gcattaacgc cttgctcgcc atcggtccac gccacaccat cgcattgtcg
     1201 tcagtgacca gataaccaat tgaattggtt gccaggccat gagacataat cggtgccatg
     1261 tgggtaccgt ccggcgacgt tggacgctga ttttctgcgc ccagcatggt gggaattgat
     1321 gggccgtaga tatcggcgtc cagaatgccg actttcgccc cttccgccgc cagtgccagc
     1381 gccaggttta ccgccgtgga ggatttaccc acgccgcctt taccggaact gaccgcaatg
     1441 atgttcttca caccattaat acccggctga ttcttcacgc gttttaaggt ggcgatattg
     1501 tgcgacagtt tccagtcgat agctttcgcc ccggtgatac gcagcagttc ggcgctacat
     1561 tgctctttta actcttcaaa cgcgctatgc cagacgaatg gcataaccag ttccacatgc
     1621 aacgtgtcgt ccatccaggc gacatggtgc aacgctttca gcgtggtgag gttatgcttc
     1681 agggttgggt gctgaaaatt ggccagtgtc ccggcgacca ttgcgcgcaa ggcttctggt
     1741 gatttggcct gggattgttc gttcatcccg actccttttt tgtatagata aaccatcagc
     1801 tgatagttta cctgaagaat atagagaagt acttacttaa cattttccca tttggtacta
     1861 tctaacccct tttcactatt aagaagtaat gcctactatg actcaagtcg cgaagaaaat
     1921 tctggtgacg tgcgcactgc cgtacgctaa cggctcaatc cacctcggcc atatgctgga
     1981 gcacatccag gctgatgtct gggtccgtta ccagcgaatg cgcggccacg aggtcaactt
     2041 catctgcgcc gacgatgccc acggtacacc gatcatgctg aaagctcagc agctcggtat
     2101 caccccggag cagatgattg gcgaaatgag tcaggagcat cagactgatt tcgcaggctt
     2161 taacatcagc tatgacaact atcactcgac gcacagcgaa gagaaccgcc agttgtcaga
     2221 acttatctac tctcgcctga aagaaaacgg ttttattaaa aaccgcacca tctctcagct
     2281 gtacgatccg gaaaaaggca tgttcctgcc ggaccgtttt gtgaaaggca cctgcccgaa
     2341 atgtaaatcc ccggatcaat acggcgataa ctgcgaagtc tgcggcgcga cctacagccc
     2401 gactgaattg atcgagccga aatcggtggt ttctggcgct acgccggtaa tgcgtgattc
     2461 tgaacacttc ttctttgatc tgccctcttt cagcgaaatg ttgcaggcat ggacccgcag
     2521 cggtgcgttg caggagcagg tggcaaataa aatgcaggag tggtttgaat ccggcctgca
     2581 acagtgggat atctcccgcg acgcgccata ctttggtttt gaaattccga acgcgccggg
     2641 caaatatttc tacgtctggc tggacgcacc gattggctac atgggttctt tcaagaatct
     2701 gtgcgacaag cgcggcgaca gcgtaagctt cgatgaatac tggaagaaag actccaccgc
     2761 cgagctgtac cacttcatcg gtaaagatat tgtttacttc cacagcctgt tctggcctgc
     2821 catgctggaa ggcagcaact tccgcaagcc gaccaacctg tttgttcatg gctatgtgac
     2881 ggtgaacggc gcaaagatgt ccaagtctcg cggcaccttt attaaagcca gcacctggct
     2941 gaatcatttt gacgctgaca gcctgcgtta ctactacact gcgaaactct cttcgcgcat
     3001 tgatgatatc gatctcaacc tggaggattt cgttcagcgt gtgaatgccg atatcgtgaa
     3061 caaagtggtt aacctggcct ctcgtaatgc gggctttatc aacaagcgtt ttgacggcgt
     3121 gctggcaagc gaactggctg acccgcagtt gtacaaaacc ttcactgatg ccgctgaagt
     3181 gattggtgaa gcgtgggaaa gccgcgaatt tggtaaagca gtgcgcgaaa tcatggcgct
     3241 ggctgatctg gctaaccgct atgtcgacga gcaggctccg tgggtggtgg caaaacagga
     3301 aggccgcgat gccgacctgc aggcaatttg ctcaatgggc ataaacctgt tccgcgtgct
     3361 gatgacttac ctgaagccgg tactgccgaa actgaccgag cgcgcagaag cattcctcaa
     3421 tacagaactg acctgggatg gtatccagca accgctgctg ggccacaaag tgaatccgtt
     3481 caaagcgctg tataaccgca tcgatatgaa gcaggttgaa gcactggtgg aagcgtctaa
     3541 agaagaagtg aaagcagctg ccgcgccagt tactggccca ctggcagatg atccgattca
     3601 ggaaaccatc acctttgacg acttcgcaaa agttgacctg cgcgtggcgc tgatcgaaaa
     3661 cgcagagttt gttgaaggtt ctgacaaact gctgcgcctg acactggatc tcggcggtga
     3721 aaaacgcaat gtcttctccg gtattcgttc tgcctacccg gatccgcagg cactgatcgg
     3781 gcgtcacacc attatggtgg ccaacctggc accgcgtaaa atgcgctttg gtatctccga
     3841 aggcatggta atggctgctg gtcctggcgg gaaagatatt ttcctgctaa gcccggatgc
     3901 cggtgctaaa ccgggtcatc aggtgaaata atcccccttc aaggcgctgc atcgacagcg
     3961 ccttttcttt ataaattcct aaagttgttt tcttgcgatt ttgtctctct ctaacccgca
     4021 taaatactgg tagcatcagc attcaactgg ataaaattac agggatgcag aatgaaacac
     4081 tttatctatc aggacgaaaa atcacataaa ttctgggcag ttgagcaaca gggaaacgag
     4141 ttgcatatca gttggggaaa agttggcacc aaagggcaaa gccagataaa aagtttttca
     4201 gatgctgcgg cagcggcaaa agcggagctt aagctgattg cggagaaggt gaagaagggg
     4261 tatgtggagc aagcgaagga taattctttg caaccttccc aaacggtaac gggctctctc
     4321 aaggtagcgg acttatccac cattattcag gaacaaccct ctttcgtagc agaaacccgt
     4381 gcgcctgaca aaaatacaga tgctgtttta ccgtggctgg cgaaagatat tgctgtcgtt
     4441 tttccgcccg aagttgtaca caccacgtta agtcatcgcc gctttcccgg agttcctgtt
     4501 cagcaagcag acaaattgac ccaattacgt cgcttagcct gtagtgtgtc gcaacgggat
     4561 aataaaacag ccacatttga cttcagcgcc tgttctttag aatggcaaaa caccgtcgcc
     4621 caggcgatca gtcagatcga cggcctgaaa acaacacagc taccatcacc agtaatggct
     4681 gtactcacgg cacttgaaat gaaatgcaca agatataaag tgcgtgagga tgttatggat
     4741 cagatcgtcc aggaaggcgg tctggaatat gctactgatg taataataca ccttcaacag
     4801 attgatatta aatgggatta tgcgaataat gtcattatta ttctgccgtc tggcattgca
     4861 cctgactact tggagcaata ttccagattt gaattacgcc tacgtaaaca tttatcactg
     4921 gcggaagagt ctctctggca aaaatgtgca caaaaactta ttgccgcaat tccacatatt
     4981 ccagaatgac ggcaaccatt aattgctttg ttattacctg aaaaaccaga aattgcacat
     5041 gaaattgccc agcgtctact ggggcaaaaa aaattaccct cgcttgagtg gttaaaaata
     5101 gtggcgactg atgagcacat tcttgcctca ttagaaaaat atcacgagcc atatgccatt
     5161 tttgatgatt actattgtgg tgcgatatgg tcagccaccg tattacagga gcaaggtgtt
     5221 gcagccctgc cccgatttgc tccctatgcc gcaagtgact actgcgccga tgtgttgcgt
     5281 catatcaatc atccgttcgc attaacactg cttatacgtg tagccgggca tactaaacgc
     5341 tgtcacgatc ggatgacgaa agccattgct gcgttcccac atgcagcaat ggcggcactg
     5401 acggaacttc ttgggcaaaa agaagagaac agttggcgca ttatgctaat gacaatgctt
     5461 atctcacaac cagcactggc agaacaggtc attccctggc tctcgacacc cgcagttgcc
     5521 gtactgaaat catgccagca acaactgaca cagccctcaa accatgccag cgccgatcta
     5581 ctgccagccg tagtagtctc ccctccctgg ctttcgaaaa agaaaaaatc gccgattccg
     5641 gtgctggatt tagcgccatt aggcattgag ccaatctgtt atctgacaga agaaatcagt
     5701 aatcaacttt tggcgaaata tatctggtat tcaaaacaca tcacggttag ccatgaagaa
     5761 agtactacca acctgttggc aaggatgggt tttcaacgac ggatcgctgg tacatatatt
     5821 aaagctcccg aagcggtagt tgaggcatgg ctaaatgaag attattcaac cttactaagt
     5881 gaatttaagg tgtttcattc acctaccggg cattattggc agttggggat tttgacaaca
     5941 ttgccgctgg agaaagcagt aaaagcatgg aatgccctta ccctatctcc acataccgat
     6001 accgaatacg ccatgttaca ttttggactc aaagggttac ctgggttagt aaactcactt
     6061 gcacgctatc cacaagaagc cttgcccatc acgaattact tcgcagcgag tgagctggct
     6121 cctgccgtcg cccgtgcctt caacaaactg aaaacgctac gcgaaaacgc ccgtagctgg
     6181 ctgttgaaat acccggaaca tgcccttacc ggcctgctgc ctgcggcgct cggcaaagcc
     6241 ggtgaagcac aggataacgc ccgcgctgcc ttgcgtatgc ttaccgaaaa cggtcatcag
     6301 ccattactgc aagaaatcgc ccgacgttat aaccagccgg aagtaaccga tgcggtgaac
     6361 gctctgcttg cgctcgatcc cttagataat cacccgacaa aaatccccac tcttccggcc
     6421 ttttatcagc catcgctctg gacgcgcccg gtattaaaag caaatgccca atcactgcca
     6481 gatagcgccc tcctccacct cggtgaaatg ctccgcttcc ctcaggaaga ggctctgtat
     6541 ccgggattat tgcaggtgaa agacgtctgt tccgccgact cactggcggg atttgcctgg
     6601 gatctgttta ccgcctggca gaccgctggc gcgccgtcga aagagagttg ggcgttcact
     6661 gcgttaggcg ttctcggtaa cgatgacacc gcccgcaaac tgacgccatt aatacgcgcc
     6721 tggcctggtg aatcccagca taaacgcgcc accgttgggt tggatattct cgctgctatc
     6781 ggtagtgata tcgcccttat gcagcttaac ggcatcgccc agaaactgaa attcaaagca
     6841 ttacaggagc gggcaaaaga aaaaattgcc gacattgccg agagccgcga actcacggtg
     6901 gcggagcttg aagatcggtt agcaccggat ctcggtctgg atgataacgg ttcgctgctg
     6961 ctggattttg gcccacggca gttcaccgtc agctttgatg aaaccttaaa accgtttgtg
     7021 cgtgatgttt ccggcagccg cctgaaagac ctgcccaaac cgaacaaaag cgatgatgaa
     7081 tcgcggtcga acgatgcggt taaccgctac aaattgctga aaaaagatgc gcgtaccgtc
     7141 gccgcccagc aggtagcaag gctggaatcc gccatgtgcc tgcgccgccg ctggtcgccg
     7201 gaaaacttcc agctcttcct ggttgagcat ccgctggttc gccacttaac ccgccgtctg
     7261 atttggggcg tttatagcgc cgaaaaccag ctactggctt gctttcgcgt agcagaagat
     7321 aacagctaca gcaccgctga cgatgatctt ttcaccctgc cggaaggcga tatctctatc
     7381 ggcattcctc acgttctgga gatatcaccg acggatgccg ccgcctttgg gcagcttttt
     7441 gccgactatg aactgttacc gccgttccgc cagctcgacc gtaacagtta cgccctgaca
     7501 gaaaccgagc gcaatgccag cgaattgacc cgctgggcag gcagaaaatg cccgagcggt
     7561 cgggtcatgg gactggcgaa taaaggttgg gtgcgtggtg aaccgcagga tggtggctgg
     7621 atcggctgga tgatcaaacc tctgggccgc tggtcgttaa tcatggaaat cgatgaaggc
     7681 tttgcagttg gtatgtcgcc agccgaactt agcgccgagc aactcttaag caagctgtgg
     7741 ctatgggaag gcaaagcaga aagctatggc tgggggagta attcaacaca ggaagcgcag
     7801 ttctccgtac tcgatgccat caccgccagc gagctaatta acgatattga agccctgttt
     7861 gaataaggaa agagcatgga caaggaatta ccgtggctgg cggataacgc ccaactggaa
     7921 ctgaaatata aaaaaggcaa aacgccgctc agtcatcgtc gctggccggg cgaaccagtg
     7981 tccgttatca ctggaagtct catccagaca ttgggagatg aattactaca acaagcaggc
     8041 cagaaggaaa atatcacctg gaattatgat aaatgctcac tggagtggca gtccgccatc
     8101 cagcaggcaa tcaatttgac tggcgagcat aaaccgtcaa tcccagccct taccatggcg
     8161 gctctgatct gtatcgcgca aaatgacagc cagcagttac ttgatgaaat cgtgcaacaa
     8221 gaggggctgg aatatgcgac ggatgtagtg atagcgcgtc agtgcattgc gcgacgttat
     8281 gagagtgact cactggtcgt cactttgcag tatcaggacg aggactatgg ctacggttat
     8341 ggctccgcga cctataacga tttcgatctc cgtttgcgta agcatctgtc gctggcagag
     8401 gaaagttgct ggcagcgttg tgccgataaa ctcattgccg cactgccagg aatacccaaa
     8461 attcgccgtc cttttattgc gctgatcctc ccggaaaaac ctgaaatagc caatgaactc
     8521 gccagtcttg aaagctctcg gtcaagtctc cattcaaagg agtggttaaa ggtcgttgct
     8581 actgataata cggcggtaaa aaaactcgaa cgatactggg gcctggacgt atttagcgat
     8641 cgcgaagcca gctacatgtc acaagaaaac cgcttcggct acgcggcctg cgcctccctt
     8701 ttgcgcgaac aaggactggc agccgttccg cgcctggcaa tgtatgccca taaagaagat
     8761 tgcggcagtc tgctggtaca aattaaccat ccgcaagtca tccgcacctt gctactggtg
     8821 gctgataaaa acaaacccag cctgcaacgt gtggctaaat acagtaaaaa cttcccccac
     8881 gcgacgctcg ccgcactggc agaactgctg gcgttaaaag aaccaccagc ccgcccaggt
     8941 tatccaatca tcgaagacaa aaagctgcct gcacagcaaa aagcacgcga tgaatactgg
     9001 cgtacgctgt tacaaacgct gatggcatcg cagccacaac tggcagcaga agtgatgccg
     9061 tggttaagta ctcaagccag ggcagtgctg aatagttatt tatcggcacc gccaaaaccg
     9121 gttattgata gcaccgataa cagcagtctg cctgaaatgc tcgtttcacc accgtggcgt
     9181 agtaagaaaa aaatgacagc tccacgtctt gatttggcac cgctcgaatt aactccgcaa
     9241 atttactggc aaccaggcga acaagagagg cttgccgcca ctgagtctgc ccgttatttc
     9301 agcacggaat ctcttgcgga acgcatggaa caaaaaagtg gacgagttgt attacaggaa
     9361 ctgggttttg gggatgatgt atggctgttt ctgaattata tactccccgg aaaactggat
     9421 gctgcacgca attcactcat tgttcagtgg cattactacc aggggcgggt tgaagagatc
     9481 ctgaatggct ggaactcccc gcaagcacaa ttagcagaac aggcgctccg cagcggtcac
     9541 atagaagcgt taattaacat atgggaaaat gacaacttct cacgttatcg tccggaaaag
     9601 agtgtctgga acctgtattt attggcacag ttgccgcgtg agatggcttt gaccttctgg
     9661 ctgcgtatca ttgagaaaaa gcatctgttc gcgggtgagg actattttct cagtatcctc
     9721 ggattggatg cgctaccagg tctgctgttg gctttttcac atcgtccaaa agaaacattt
     9781 ccgttaattt taaattttgg cgcaacagaa ctggcgctgc ctgttgcccg cgtctggcga
     9841 cgtttcgcgg cgcagcgtga tctggctcgc cagtggattt tacaatggcc ggaacatacg
     9901 gctagtgcac ttatccctct tgtctttacc aaacccagcg ataatagcga agccgcatta
     9961 cttgccctgc gtttactgta cgaacaggga catggcgaat tgctacaaac cgtggcaaac
    10021 cgctggcagc gtacagatgt atggtctgcc ctggagcagt tgcttaaaca gggtccaatg
    10081 gacatttacc cggcacgcat tccaaaagcc cctgatttct ggcatccggc aatgtggtcc
    10141 aggcctcgac ttatcactaa taatcagcct gttaccggtg acgctctgga aattatcggc
    10201 gaaatgctgc gctttaccca ggggggacgt ttttatagcg ggctggaaca actgaaaacg
    10261 ttctgccagc cacaaacgct ggcagctttt gcctgggatc tcttcactgc gtggcaacaa
    10321 gctggtgccc ccgcaaaaga caactgggca tttctggcgt taagtctctt tggtgacgaa
    10381 agcacggcac gggatctgac gacacagatc ctcgcctggc cacaagaagg caaatctgcc
    10441 cgtgctgtca gcggcctgaa catccttacc ctgatgaata atgatatggc gctgatacag
    10501 ctgcatcata tatcgcaacg ggctaaatcc cgccccttac gtgataacgc ggcggaattt
    10561 cttcaggtag tcgcagaaaa tcgcgggcta agccaggaag agctagcgga cagattagtc
    10621 ccaaccctgg gccttgatga tccgcaggcg ttgagttttg attttggtcc ccggcagttt
    10681 accgttcgct tcgatgaaaa cctcaacccg gttatcttta atcagcaaaa cgttcgccag
    10741 aaaagcgttc cccggttgcg cgccgatgac gatcaactga aagcgcccga ggcactggcc
    10801 cgactaaaag ggctaaaaaa agatgctact caggtgagca aaaacctgct cccgcgtctt
    10861 gaagctgccc tacgtaccac ccgacgctgg tcgctggcag attttcattc tctgtttgtt
    10921 aatcatccct ttacccgtct ggttacccag cgattaatat ggggggttta tccggcaaat
    10981 gaaccgcgtt gtttactcaa agcctttcgt gtggccgcag agggggagtt ctgcaatgcg
    11041 caagatgagc caattgacct gcctgcggac gctctgattg gcattgccca cccgttagaa
    11101 atgacagtag aaatgcgcag tgaatttgca cagctttttg ccgattacga aattatgccg
    11161 ccttttcgcc agttgtcgcg ccgcacggtg ctgctcacac ctgacgagtc aaccagtaac
    11221 agcctgactc gctgggaagg taaatccgct accgttgggc agcttatggg aatgcgatac
    11281 aaaggctggg agtcaggtta tgaggacgca tttgtctatg acctgggtga gtaccggctg
    11341 gtccttaagt tttcacccgg ttttaaccac tacaatgttg atagcaaagc gctaatgagc
    11401 ttccgttctc ttcgagtgca ccgtgacaat aaatccgtca cttttgccga acttgatgtg
    11461 tttgatttga gtgaggcgtt aagcgcacct gacgtcattt tccattaaaa acacagcggg
    11521 cagtgatgca actgcccgtt atcaacacga cttaccagcg gggaaagcga tgatcgtgca
    11581 aaaagagctg gttgctattt acgattatga gatccctgta cctgaaaatc cgttttcctt
    11641 cagacttgag atccataaat gctctgaatt atttacaggt tccgtctatc gactggagcg
    11701 attccggcta cgtccaacat ttcatcaacg tgatcgagaa gatactgacc cgctaataaa
    11761 tgatgcgttg atttatataa gagatgagtg tattgatgag cggaaattac gaggtgaatc
    11821 acctgaaact gtaatagcaa tttttaatcg tgaactacag aatatattca accaagaaat
    11881 agaataatat actctagata attaaagtcg cagcacatgc aacttgaagt atgacgagta
    11941 tagctttctt aaagggggaa acggtattcc cccttagatg cgtttcaatt tgcccgttac
    12001 ttcaacaaat caatcaaccg taacagagcc actaataact ccagtacggc aatgagcacg
    12061 gttaactttt gtgtcagcgt gtccatccgc tctccttatt aaggagcgca acaccagcac
    12121 cttaccctta cactgcctgt tgatactggt ttacgtatgt tggtacgaaa cgttgctctc
    12181 cggatcaggg caccgcctgc ggcaccaccc gtccggcagc caggacttaa aattggtccg
    12241 atccggcaca acctcccaac gggcaggatt ataaatctca ctcactgaat tgcaatggag
    12301 tgtggatatg gttataattt attcgttggt acgaaatgtt gcacaatcag caagttagcg
    12361 tcactgtagt aaataaaaga acctgcctca cc
//
LOCUS       DAZGLJ010000132        12358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000132 DAZGLJ010000000
VERSION     DAZGLJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.132"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(46..1470)
                     /gene="patD"
                     /locus_tag="ACHK49_003239"
     CDS             complement(46..1470)
                     /gene="patD"
                     /locus_tag="ACHK49_003239"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO9263847.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(1492..2286)
                     /gene="ydcV"
                     /locus_tag="ACHK49_003240"
     CDS             complement(1492..2286)
                     /gene="ydcV"
                     /locus_tag="ACHK49_003240"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9263848.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2276..3217)
                     /gene="ydcU"
                     /locus_tag="ACHK49_003241"
     CDS             complement(2276..3217)
                     /gene="ydcU"
                     /locus_tag="ACHK49_003241"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9263849.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPRLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(3218..4231)
                     /gene="ydcT"
                     /locus_tag="ACHK49_003242"
     CDS             complement(3218..4231)
                     /gene="ydcT"
                     /locus_tag="ACHK49_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9263850.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(4249..5394)
                     /gene="ydcS"
                     /locus_tag="ACHK49_003243"
     CDS             complement(4249..5394)
                     /gene="ydcS"
                     /locus_tag="ACHK49_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO9263851.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(5639..7044)
                     /gene="ydcR"
                     /locus_tag="ACHK49_003244"
                     /pseudo
     CDS             complement(5639..7044)
                     /gene="ydcR"
                     /locus_tag="ACHK49_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            complement(7123..7539)
                     /gene="hicB"
                     /locus_tag="ACHK49_003245"
     CDS             complement(7123..7539)
                     /gene="hicB"
                     /locus_tag="ACHK49_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO9263852.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(7585..7761)
                     /gene="hicA"
                     /locus_tag="ACHK49_003246"
     CDS             complement(7585..7761)
                     /gene="hicA"
                     /locus_tag="ACHK49_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFO9263853.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            7983..8213
                     /gene="yncJ"
                     /locus_tag="ACHK49_003247"
     CDS             7983..8213
                     /gene="yncJ"
                     /locus_tag="ACHK49_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO9263854.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            complement(8305..10266)
                     /gene="rlhA"
                     /locus_tag="ACHK49_003248"
     CDS             complement(8305..10266)
                     /gene="rlhA"
                     /locus_tag="ACHK49_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO9263855.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFFANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(10339..10875)
                     /gene="sutR"
                     /locus_tag="ACHK49_003249"
     CDS             complement(10339..10875)
                     /gene="sutR"
                     /locus_tag="ACHK49_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO9263856.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            10928..12142
                     /gene="ydcO"
                     /locus_tag="ACHK49_003250"
     CDS             10928..12142
                     /gene="ydcO"
                     /locus_tag="ACHK49_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO9263857.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            complement(12182..>12358)
                     /locus_tag="ACHK49_003251"
     CDS             complement(12182..>12358)
                     /locus_tag="ACHK49_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO9263858.1"
                     /translation="RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     PLKQEPTEMIQATA"
BASE COUNT         3010 a         3324 c         3043 g         2981 t
ORIGIN      
        1 cctaaaacga tagtggccgt cctataacag ccactaacat ctaacttaat gtttaaccat
       61 gacgtggcgg acgacggtgt aatcctccag cccataaagt gacatatcct tgccgtaacc
      121 agaaagtttc tgcccaccgt gcggcatttc acttaccagc atgaaatggg tattgaccca
      181 ggtacaacca tattgcagcc gtgcgctgac gcgatgcgcc ctgcccacat ctttcgtcca
      241 taccgaagat gcaagtccgt actggctgtc attcgcccag ttcaccacct gttcttcgtt
      301 gtcgaagggc gtaacactca ctactggacc aaatacctct ttttgcacga tggcatcgtc
      361 ctgtaatgcg ccagccagca gcgtcggcgc atagtaatag ccattaccct tgcgcttttc
      421 accgccagtg atcactttga tgtgccctgt cgctttcgcc tcttctactg ccttgctgac
      481 gcgttcgaga tgcgccagcg agcttaaagg tccaagctcc gtagactcgt catccggcgc
      541 accagatttt aacgttgcca ccgcagcacc cagtttttcc accagcgtat cgtaaatgcc
      601 tttttgcgcg tagatccgac aagccgcagt acaatcctgt ccagcattgt aatagccaaa
      661 tgtacgtaca ccttcgacca ctgcttcaat atccgcatca tcaaaaacaa tcactggcgc
      721 tttgccacca agttccatat gagtacgctt aatggacgac gcggtatggc tgatgatgtg
      781 ctcgccggtg gcgatagagc ccgtcagcga caccatccgc actttgggat gaccggtcag
      841 cggatcaccc accgttttgc ctctgccaaa cagtacgtta atcacgcctg ccgggaagat
      901 atctttcgcc agctctgcca acttcaacgc ggtcagcggg gtaatttctg atggtttaag
      961 cactacgcag ttccctgccg ccagcgccgg agccagtttc cacgctgcca tcatcagcgg
     1021 ataattccac ggtgcgatag aagccacgac ccccaacgga tcgcgacgga tcatcgaagt
     1081 atgaccttca agatattcac ctgccgccag accattcaga cagcgcgccg cacccgcgaa
     1141 aaagcgaaaa acatcgacaa tcgccgggat ttcatcattg aacgcactat gcagcggttt
     1201 gccacaatta cgggactcca gttcggcaaa aacctgacca ttttcttcga taacatcagc
     1261 cagtttcagc agacattccg cacgcacttt cggcgtggtt tgcccccatt cggcaaatgc
     1321 tgcatctgcc gcgcgcacag cagcatcgac ctgctctgcg gatgcctcgg caatctccag
     1381 taaaacgtcc cccgttgccg gattatagac aggctgtttt tccccttcgc cgctaaccag
     1441 ttctccgtta atcagtaact tatgttgcat agcatattcc tgtatcagtg gttattgacc
     1501 attgtcgcct tcgcgggtta gccaccaggc ccccaggatc ggcaaggttg ttaccaacat
     1561 aaccagcagt gccaccacgt tagttaccgg tacatcacgc ggtcgcccaa gctgattgag
     1621 caaccacaac ggtaacgttc gttcatgacc tgccgtaaaa gtcgtaacga tgatttcatc
     1681 gaacgacaag gcaaacgcca gcattcctcc tgccagtagc gccgaactga gattcggcaa
     1741 cactacgtag cggaaggttt gccagccatt ggccccaaga tccattgacg cctcaaccag
     1801 actccacgag gtgcggcgaa aacgggcgat gacattgtta aacaccacca ctacgcaaaa
     1861 agtcgcatga ccgaccacga tggtgaaaaa tcccggctcc agattgatgg ttttaaaagc
     1921 ggttaatagc gccagaccag tgacaatgcc cggcagcgca atgggcagca gcagtaacag
     1981 cgaaatggcg tttttgccga aaaagtctcg tcgccacagc gcggcagctg ccagcgtccc
     2041 taacaccagc gccattaatg tcgccagcgc cgccacttta agtgacagtg tcacggcatc
     2101 aagaatatca ctacgctgtg ctgccacgct aaaccagcgc agcgtcaggc cctgcggtgg
     2161 aaaactaaac gccgcatctt cagtgttaaa cgcataagcg gcgataatca ggatgggaaa
     2221 atgtaggaaa accacgccgc cccaggccgc cagtttgagg aaaaacggtg cgcgttcaga
     2281 gtgcatcgaa cgctcccaga cgtttcacga acgccaggta cagtgcgatg agaacaatcg
     2341 gcaccagggt gaatgccgcc gccatcggca tattgccaat cgccccctgc tgggaataaa
     2401 ccatattgcc gataaaatat cctggaggac caaccagctg cgggacgata aaatcgccca
     2461 gtgtgagtga gaaggtaaag atagagccag cggcaatacc cgggattgcc agcggcagca
     2521 ccacatagcg aaaggtttgt cgtggacgtg cgccgagatc agccgacgcc tgcaacaatg
     2581 acggcggcaa acgctcaagc gccgcctgaa cgggcaggat catgaacggc aaccagatat
     2641 agagaaacac cagaaagcgc cccagcccgg aagttgacag cgtatttccg cccaccgcag
     2701 gtaatgtaag gaacgcagtc agcagtggtt ccagcccaag atgttgtaaa aaccactgag
     2761 ccacgccatc ttttgccagc aataacgtcc aggcataggc tttaacaatg tagctcgccc
     2821 acatcggcaa cattaccgca atataaaaaa aagctttcat tttcccgctg gtatagcgcg
     2881 ccatatacca cgccattgga aaagccagaa tggcgctggc gatagtgact gccacagcca
     2941 tagtcagcgt gcggagaatg atgtcgtaat tcgccggatt aaacagcgca cggatattcg
     3001 ccagcgtcag ttccggcgtt accgacatgg tgaaatcgtc aaaagtataa aatccctgcc
     3061 ataacagtgt cagcagcgag ccgaaataga caatgccaaa ccacattagc gggccaagca
     3121 gcagtaaaaa cagccccagc ctcggattac gccagaagaa accggacacc ttacccagac
     3181 ctggacgtga aggtgattgc aatacattca tcgccattca cctctcctca accagcggca
     3241 ccatcacatc acgcgaccag gaaaccatca cctgttgtcc gggcgtgagc gtggcaggca
     3301 gttcttcgcc tgtcatattg gcctgactca caagcagttt ttcaccgccg ttcaatttca
     3361 gttcaaaacg agttgccgcg ccctgatatt gcaccgcctg gatcgtgcca ttggcctgca
     3421 gttcaccagg ggtgttgagg cggatatgtt ccggtcgcag ggcgaagctt cccgtcatgc
     3481 cacaaagttt ctctgccatc agtccatcaa aaacgttgga tgtcccgacg aatccggcaa
     3541 caaacggcgt gcgcgggcgc atatagagat cgcgcgggga atcgacctgc tcaatgcgtc
     3601 cattattgaa aaccgccaca cgatcggaca tcgataacgc ttcgccctga tcgtgggtaa
     3661 cgaagataaa agtgataccg agagactgtt gcagtttttt cagttccagc tgcatctgct
     3721 cacgcaattt gagatccagt gcgccgagcg gttcatccaa cagcaatacg cgcggttcat
     3781 tcaccaatgc tctggcgata gcaacccgct ggcgctgacc accagaaagt tgtgacggtt
     3841 tacgttgatg tacaaacccc aacgccactt tctccagcgc ctcttgcgcc attgcgtgcc
     3901 gctgcttttt attcacgcct ttgaccatca gcccataggc gacattgtca agaatcgaca
     3961 tatgcggaaa tagcgcgtag tcctgaaaga cagtattcac gtcccgctcc cacggtggca
     4021 gattgctggc gggtttacca aagatagaga tagccccgcc ggaaagctgt tcgaagccag
     4081 caatcaggcg caggcaggtg gttttgccgg agccggacgg ccccagcata gagaagaact
     4141 caccatcttt tatcgcaata ctgacgccat ctactgcgcg cacgtcaccg tacaaccgcg
     4201 agacgttgtc aaactccact gcgtacgtca taaagcaccc cagcgaagtt agcgaccgcc
     4261 cataatggca atgtaatcct gcgtccagcg actgtaggga acaaacttgc ccccttctgc
     4321 tataggcgtt ttccagaagg cgattttgtc gaaatagtta aaaccgttgg tttcacaacc
     4381 tttttcgcct aataacggac tggctttaca cccttccggc actaccggta acgagccaaa
     4441 ccaggccgcc acatcgccct gcacttttgg cgttaatgac cagttcatcc atttgtaggc
     4501 gcaaaccgga tgtttcgctt cgctatgcag catggtggta tcagcccaac cggtaacacc
     4561 ctccttcggg aaaacggtag caacaggctg gccttcggct ttcagggcgt tggcctgata
     4621 gggccaggca ctggaagcaa ccacaccctc gtttttgaaa tcgctcattt gcacggtagt
     4681 gtcatgccag tagcgatgga tcaaactgtg ttgagcgcgc agcactttca gcaccgcctg
     4741 gtactgttct tcggttagtt gatacggatc gctgatgccc aactgcggct gagtggcttt
     4801 aacgaacaac gcagcgtccg caatgtagat agggccatca taagcctgaa cgcggccttt
     4861 attgctcttg ccgtccggca gattttgctc aacaaaaacc acttgccagc tatccggcgg
     4921 cgtcgggaag gttttagtgt tgtacatcag caggttcggc ccccattggt aaggtgtgcc
     4981 gtaaactttg ccgccaacat taaaccagtc gcctttaacc acgcgcggat cgagcgtttt
     5041 ccagttggga atcaaggcgg tattaatcgg ctgcacgcgt ttgcccataa tcaaacgcag
     5101 cgaggcatcg ccggatgccg taaccagatc gtaaccccct ttggtcatca gactgaccat
     5161 ttcatcggaa gtcgcggcgg ttttcacatt caccgcgcag cctgtctctt tttcaaattg
     5221 cgttacccag tcgtattgtt tatcagtttg tccgcgttcg atgtatcccg gccaggcgat
     5281 aatatccagt cgcccttccg gtttatctaa attggtaggt ggttcggcgg cgtgagcggt
     5341 cattattgtc atggtgagcg cacacaggct gctgcgggca aatgtcttgc tcataaggtc
     5401 ttactcctgt cgtaattaat tgttcagccg ccgtgccgta aaaatatctc cttgtttaac
     5461 catagacggc ggcttgcagg cacaccttcg cccctgagaa aatttaatca ggttgtgagc
     5521 taccgcgcat tatgccgtct gtaaggttat cttctggagt atagacaagg ggttaggaaa
     5581 tatgactgct attcgaaatt catattaccc aatctaagag gaattaaatt aaacgctatt
     5641 acagccgttc ttgaataagt ttgcctaatt gttttaccgc ctgttcttca cgttctcccc
     5701 actgccacgc ggtattaaaa cgaaaaaaac gtgaccagtt ttcaccggta gaaaacattt
     5761 tacccggcgc aatactgata tgatgcgtca gtgccgccag gcttaattcg ccggcatcta
     5821 acggctcggg gagctccagc cagagaaaat aaccgctgtc actatgatga attttaactt
     5881 ccgcaggcag ataacgcagc agtgcctgcc aggcacgttg tttacgttcc gcaagctggc
     5941 gacgcaggcg acgaagatgg gcgtcgtatc ggcgcgtgga aaggtaatcc accagcgcaa
     6001 gctgcatcgg tgagctggtg gaaagcgtac tcatcaactg caagcgttga attttacgtg
     6061 catgttttcc ggcggcgacc caaccaatac gaaaaccagg caccagacat ttcgaaaacg
     6121 aagagcaatg caaaacgcca tcgtggcgat cccacgcttt cgcaggcagc ggtttttccc
     6181 gtccaaaata aagttcgctg taaacgtcat cttcaatcag cgttacgttg tactgattga
     6241 gtaacgccac cagttgtgct ttttttgcgg cgttaaggta aatccgagtg gattctggct
     6301 attagtcatc agccagcacg ctttcaccgg atactcctgc aacgccagtt ccaacgcctg
     6361 aagatctatc ccttctttaa catccgtcgc caccgataac gctttcagcc gtagccgttc
     6421 cagcgcctgc aacgcgccat agaaacaagg attctctact atcacccaat cgcccggttc
     6481 agttaccgct tgcaaactga ggtttaatgc ctctaacgcc ccggcagtaa tgacaatttc
     6541 atcaggagaa atggtgatgc cctgtaaggc atagcgacga gcaatagcct gacgcagttc
     6601 tgcgtttcct ggcggtaagt tttcaatcac gctcatcgcc gtggcggttt tgcttacctg
     6661 cgccagcgag cggtttagtt gttggagggg gaaaagtcgc gggtcgggaa aggccgaggc
     6721 aaacggaacg accgacggat cgcgactggc ctgcaacata tcaaaaatat aagtgttgat
     6781 atcgactgct tcatctcgag tgactggaat gactggcgct ttcggcattt ttattgcctg
     6841 tggcgcaacg taataacccg actgcggtcg tgcgataata tatccctgac tttcgagcaa
     6901 ctgataggca tggctgacag tcataaagct catgcctgaa agcgccacct ggtcacgcaa
     6961 cgaaggcaaa cgatcgccgg gttgccagat acccgacgca atctgctcgc gtaattgttc
     7021 tgcaagctgc tggtattttt tcattatcga atcgtaatta tgtgcagatg attcggcagt
     7081 ctatatcagt tgttaaaaat gacaactttt cgttaactgt aattaaacca tcaccagcga
     7141 taactctttg ccaagcgcct ttgccgcaag ctggacggcg tcgatttttg tcgcatggtg
     7201 caagttaaat aggcgagtaa tctcctgttt aggtttgcca attcgcctgg ctaactcttg
     7261 ctgagtgatt tctgactgta aaaaagcatt taacagcaat actttagagg cgacgctcaa
     7321 aggtacttca ataaagtgat catgactatt taatggcgaa ggtaacggga taagctcgtt
     7381 atcttcaaaa taaaaatcaa atgcggtcag taaagcatct tttgccgctt ccatcgcttc
     7441 agcgacagtt tcgccctgcg tcaacgcttc agggatatcc acaaaagaaa ccatataacc
     7501 gccttccggc gcgggtgtaa gagtgacggg ataacgcata atttcgactg tccctcgcat
     7561 gtcattaacc tttttaattg gcgattaact caaaccgagt tgtttcagga ttgctttacg
     7621 caatggttct ttaatctcat cgcaggggtg acgcggcatg acactgcgcc tcccatgaaa
     7681 cctgagtttc aaatggttgc tgccattcgc tacatcgacg ccctgagatt cgagccaacg
     7741 tctgaactcg ctttgtttca catcgcctcc ctgccgctga gtatatcaac aaagtaaaca
     7801 ttagtgttta cctccgtgtg taatttgctc gaaaatagca ctaaatcgcc gcaacggcag
     7861 aatgccatgc aaaatcagga agaaagactc cagaatttct ttgcgctaat ttgcaaaact
     7921 gaataataac ctcaagaaac gatcggcatc atagcggcaa aaggtaagaa aaaggtggca
     7981 ggatgtttac gaaggcgtta tcggttgtct tattaacgtg tgcgctgttc tcaggacaac
     8041 tcatggcagg gcacaaagga catgaatttg tgtgggtaaa gaatgtggat catcagctgc
     8101 gtcatgaagc ggacagcgat gaattgcgtg ctgtggcgga agagtcggcg gaaggtttgc
     8161 gcgagcattt ttactggcaa aaatcgcgca aaccagaagc gggacaacgt tgagtcattt
     8221 gttgccggat gcggcgagac gccttacccg gcaggcaata tcatgcagat tcaatgagtt
     8281 gtgagtaacc agaaaactgg cgttttaccc ttttcgctta ggcaatgttt tcagcagctc
     8341 atccggactt acggatgcca ctacgcttcc cggtaacggc attttcagta tgtgattttt
     8401 gattttgcca atgacgtgca tctcgcatgg gcggcaatca aactttagcg ttaatacttc
     8461 atcgccgtta accagttgca ttggcgtcgc cttccagctt ttgatattgc ctttcgcctg
     8521 tttcgggcac agattaaagg caaagcgcag acaatgcttg gtgatcatca ccgggacttc
     8581 gcccttctct tcatgtgctt catacgccgc gtcaatcagc tgcacaccat agcgatgata
     8641 aaattcacgc gctttctggt tgtatacgtt cgcgaagaaa ctcagatgcg tttgcggata
     8701 aaccggcgca ggatcagcaa ccggtttacg gctgccgcgc tggtaactgg caagacgcgc
     8761 agcatccagc atgtcagcag cttcacggcg gaactggttt aacagactgt ttggtacaaa
     8821 cagcgccccc ggcaaattaa tttgcacatc gcgggcgtaa tagagggttt gccccagttt
     8881 tgccagacca tccttcagat tgttcattgc tttttcggcg ttattggctt cgtcgaactg
     8941 cccgtccagc gtatgcgtga tgctgacacc ctcttcactg gtgagggtca gaatcagttg
     9001 ttcctgccag ccgcccagtt caatgtctac cgccacccga cgttcgctgg aggtttttgt
     9061 cagtgcctgc tgccagttat gatcaagatt acggtttagt gggtgatgtg gacgaatttt
     9121 gtgcaaatct gctggcattt cattgggcca gacgcggtac tgattttctc cggttttctc
     9181 gaccgtattg gcacgaaaac cgacgacttc acgtttaatc atcacgttca ggccatcacc
     9241 atttgccagt ggctcggtga cggcaacatc gagatgatct ttcgccactt tcaatacttc
     9301 gcctaccggc aggccgataa atttcggcga atcgaatgcg ccaatatcgc ctttacgggc
     9361 attcacaaaa taatctgtgc taccacggtg gaaagtcttt tccgtcgatg gaacaaagaa
     9421 atgttcagta cgacctgatg aagcgcgcgc cagatcgcca cgttcttcaa taatggcatc
     9481 aagcatctgg cgataatggg cggtgatatt cttcacgtag ctcatatctt tgtaacgccc
     9541 ttcaatcttg aaggagcgta cgccagcatc aatcagcgcg ccgaggttgg cagtctgatc
     9601 gttatctttc atcgacagca gatgtttttc ataggaaacc acccgcccct gatcgtcttt
     9661 caatgtgtat ggcaaacggc acgcctgcga gcaatcacca cggttggcgc tacgccctgt
     9721 ttgcgcatga gaaatgtagc actgacccga ataggccacg cacagtgccc catgaataaa
     9781 gaattcaatg gtcgcgtccg tggcctggtg aatcgcgcgg atctgctcaa gattcagctc
     9841 tcgcgccagc acaatctgcg tgaagccaac atcagagagg aacttcgctt tttctactgt
     9901 acgaatgtcg cactgcgtac tggcgtgcag ttcaatcggc ggaatatcaa gttccagaat
     9961 ccccatatcc tgaacaatca gcgcatcgac accggtctgg tagaggtcgg taatcagccg
    10021 ttgcgcgggt tccagctcat catcatgcaa aatggtgtta agcgtgacga aaatttttgc
    10081 accataacga tgggcaaacg gcaccagctc ggcaatatct ttcaggctat tactggcatt
    10141 atgacgggca ccaaaaccag ggccgccgat ataaacagca tcggcaccgt gcaaaatagc
    10201 ttcgcgggca atggcggcat cgcgtgccgg gcttaacagt tcaagtcgat gagaagatac
    10261 ggtcacattc ttatccagta gctgaaaaat ggcggctatt ttagccacaa gcgcgacgtt
    10321 gcgaaatagt tttcccgctt aactgcgcgg gtaatggatg agggaatgaa aatgtacggt
    10381 ttgctcccca ccattacgat agatatgcgt gacgtctgca gcaaatcgaa cgccttcccc
    10441 acaattaagg gtctgccatt ccccatcaac gcacagatca agttgtccat caatgacgac
    10501 gacatgttcg attaccccct tctcatgggg cgtcgattcg cttattgcac cagatgccat
    10561 ctgaatcgag aagtgttcga agcagagttg tggatcataa ggaaacagcg aagtgatcac
    10621 catcgcctgc tgttgtggat cataaactga aggcgtagcg gactgcggcg gagaaataaa
    10681 tgttgaaaat ggtacgttca acccggtggc aattttccat aatgtcgcga ccgtcggact
    10741 ggactcatta cgctcaatct gccctaacat tgctttagaa acgcccgtcg cttctgccag
    10801 tcgcgaaaga ctccagccgc gctgctggcg tagttgttta agtgtggtgg ataaaaagcg
    10861 agcgagattt tccatgcgac tcctccggca aaacggaagt ttatcacttg tgcgttataa
    10921 cggacaaatg ctacggtgcc tgtacgctat aacgcacgag gtgactatgc gtctgttttc
    10981 tattcctcca cccacgctac tggcggggtt tctggcggta ttaattggct acgccagttc
    11041 agcggcaata atctggcaag cagcgattgt cgccggagcc accactgcac aaatctctgg
    11101 ctggatgacg gcgctggggc tggcaatggg cgtcagtacg ctgactctga cattatggta
    11161 tcgcgtacct gttctcaccg catggtcaac gcctggcgcg gctttgttgg tcaccggatt
    11221 gcagggacta acacttaacg aagccatcgg cgtttttatt gtcaccaacg cgttaatagt
    11281 cctctgcggc ataacgggac tctttgctcg tctgatgcgc attattccgc actcgcttgc
    11341 ggcggcaatg cttgccggga ttttattacg ctttggttta caggcgtttg ccagtctgga
    11401 cggtcaattt acgttgtgtg gaagtatgtt gctggtatgg ctggcaacca aggccgttgc
    11461 gccgcgctat gcggtaattg ccgcgatgat tattgggatc gtgatcgtta tcgcgcaagg
    11521 tgacgttgtc acaactgatg ttgtctttaa acccgttctc cccacttata ttacccctga
    11581 tttttcgttt gctcacagcc tgagcgttgc actccccctt tttctggtga cgatggcatc
    11641 gcaaaacgca ccgggtatcg cagcaatgaa agcagccggg tattcggctc ctgtttcgcc
    11701 attaattgta tttactggat tgctggcact ggttttttcc cctttcggcg tttattccgt
    11761 cggtattgcg gcaatcaccg cggctatttg ccaaagcccg gaagcgcatc cggataaaga
    11821 tcaacgttgg ctggcagctg ccgttgcagg cattttttat ttgatcgcag gtctgtttgg
    11881 tagtgctatt accgggatga tggctgccct gcccgtaagt tggatccaga tgctggcagg
    11941 tctggcgctg ttaagtacca ttagcggcag tttgtatcag gcgctgcata atgagcgtga
    12001 gcgcgacgcg gcggtggtgg catttctggt aacggcaagt ggattgacgc tggtcgggat
    12061 tggttctgcg ttctggggat taattgccgg aggcgtttgt tacgtggtgt tgaatttaat
    12121 cgctgacaga aaccgatatt gacatcctcc acgccctgaa ggacgaggat ccctgctacg
    12181 ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt caacgggcgg cctgactgca
    12241 ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa aatgttacga gcgccattta
    12301 catcggcgtt cgctgtatat ccacatacct ggcatctgaa tttactttgt gacaggcg
//
LOCUS       DAZGLJ010000133        12190 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000133 DAZGLJ010000000
VERSION     DAZGLJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.133"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..811
                     /locus_tag="ACHK49_003252"
     CDS             <1..811
                     /locus_tag="ACHK49_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HFO9263859.1"
                     /translation="IQTESQPENESQDDNGKEMATDPEKTEETPEDYALRIGDEEIQL
                     NADDNDHIDGQPAPQWVKDLRKGFKETQKENRELRRQLEEALAKPAEHQQPQPDAIPP
                     KPTLESCDYDEQAFEQALTDWHEKKGRVEQQQQQKLRQQQEYQQRFQQRVEAHKQRAA
                     KLPVKDYQEMEAIVLSELPPIQQEIIIHCADEGSELLAYGLGKSQQLRQRVAAETDPI
                     RAAFLLGQISKQVSLAPKPKKAIKPEPEVRGGGADAKQDEFNKLCPGAKIE"
     gene            823..2094
                     /locus_tag="ACHK49_003253"
     CDS             823..2094
                     /locus_tag="ACHK49_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFO9263860.1"
                     /translation="MPNNLDSNVSQIVLKKFLPGFMSDLVLAKTVDRQLLAGEINSST
                     GDSVSFKRPHQFSSLRTPTGDISGQNKNNLISGKATGRVGNYITVAVEYQQLEEAIKL
                     NQLEEILAPVRQRIVTDLETELAHFMMNNGALSLGSPNTPITKWSDVAQTASFLKDLG
                     VNEGENYAVMDPWSAQRLADAQTGLHASDQLVRTAWENAQIPTNFGGIRALMSNGLAS
                     RTQGAFGGTLTVKTQPTVTYNAVKDSYQFTVTLTGATASVTGFLKAGDQVKFTNTYWL
                     QQQTKQALYNGATPISFTATVTADANSDSSGDVTVTLSGVPIYDTTNPQYNSVSRQVE
                     AGDAVSVVGTASQTMKPNLFYNKFFCGLGSIPLPKLHSIDSAVATYEGFSIRVHKYAD
                     GDANVQKMRFDLLPAYVCFNPHMGGQFFGNP"
     gene            2137..2322
                     /locus_tag="ACHK49_003254"
     CDS             2137..2322
                     /locus_tag="ACHK49_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263861.1"
                     /translation="MDRMSVFLAADNESGHVQAVIAEKDFQFFERLGFVASVDELKPT
                     SKRGRKAADNGNSTDKG"
     gene            2297..2779
                     /locus_tag="ACHK49_003255"
     CDS             2297..2779
                     /locus_tag="ACHK49_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFO9263862.1"
                     /translation="MATVLTKGEIVLFALRKFAIASNASLTDVEPQSIEDGVNDLEDM
                     MSEWMINPGDIGYAFATGDEQPLPDDESGLPRKYKHAVGYQLLLRMLSDYSLEPTPQV
                     LSNAQRSYDALMTDTLVVPSMRRRGDFPVGQGNKYDVFTSDRYYPGDLPLIDGDIPNA
                     "
     gene            2788..4206
                     /locus_tag="ACHK49_003256"
     CDS             2788..4206
                     /locus_tag="ACHK49_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HFO9263863.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPADSGFMQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDPEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYGLQQEHLLFTPLFKADNARCF
                     DLEVESSTGVAQYADRLFLSATTDGINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            4206..5054
                     /locus_tag="ACHK49_003257"
     CDS             4206..5054
                     /locus_tag="ACHK49_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail needle knob protein"
                     /protein_id="HFO9263864.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHESRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNSATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            5054..5509
                     /locus_tag="ACHK49_003258"
     CDS             5054..5509
                     /locus_tag="ACHK49_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFO9263865.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VYGQFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            5512..6204
                     /locus_tag="ACHK49_003259"
     CDS             5512..6204
                     /locus_tag="ACHK49_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO9263866.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            6214..7620
                     /locus_tag="ACHK49_003260"
     CDS             6214..7620
                     /locus_tag="ACHK49_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFO9263867.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIDRS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLASSSPEAMQSWLQNNAKELTRVGVDPNSVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQ
                     ARGQNLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGI
                     ETAVNNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQ
                     NALVNSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNE
                     FKAEWMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEP
                     RNQVGSYTSKSGIQFTVE"
     gene            7620..9464
                     /locus_tag="ACHK49_003261"
     CDS             7620..9464
                     /locus_tag="ACHK49_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO9263868.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTRDEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDKPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDSLHEVLQKTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNALLRLANTKAGTPAYERALNNAANAIRPILSSQITAEQQ"
     gene            complement(9479..9964)
                     /locus_tag="ACHK49_003262"
     CDS             complement(9479..9964)
                     /locus_tag="ACHK49_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263869.1"
                     /translation="MKKSLLIIPLLLVGCAKVSDYQASCEQRYQKLSDMANCLDASVK
                     NDSRMASAPTPKLYVLAAKMLGKGVDEGKISDAQARLELQNLYVQLQSQEQAQQIAQS
                     QAFQQALLNYQAVNTMQAIEQKARQPVITQPYPTRVDTYTNCNSGFGNTVTCNSSSNI
                     R"
     gene            complement(10035..10301)
                     /locus_tag="ACHK49_003263"
     CDS             complement(10035..10301)
                     /locus_tag="ACHK49_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO9263870.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKMVFETLKTMYSNNKKET"
     gene            10423..>12190
                     /locus_tag="ACHK49_003264"
     CDS             10423..>12190
                     /locus_tag="ACHK49_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tailspike protein"
                     /protein_id="HFO9263871.1"
                     /translation="MSDITANVVVSMPSQLFTMSRSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVSQPIIINAAGYPVYNGQIAKFVTVQGHSMAVYDAYGAQQFY
                     FPNVLKYDPDQFKGNLLSEDHSLGDSLVMHNPQFVDSTSTILSKKLTIDLRTLTDYGF
                     AEGNTGAQNKAAFQKAIDDATLPTEIVIPEGAFIVDPGITIKNTVTMIRGAGAYQSRI
                     FSTGTAAPIITQQDGVITFCEFRDFGLDGNGYAANGISLTEANHIKIENIDVVNTNNN
                     AILVNGYSIDIIGCRLFQNAGNGINVGGYCNNINIINSRIYGNGAGGVLLTPAYAEGG
                     MSVRVNGNNIEQNKFYGLLAYGVKGLNLDANYWERNGEIGYPYSVPESITVRADIHLI
                     ANNFTLIPDLSKINDTVSIRGNQQTAIGYASALPNQDGFIFTNYAKNLTIENNQLLDA
                     SKVNNLLAMYHNNLSSKVTDRLYLANNTVNSIGYVGSYDPATQNPDTAHLIDIANREL
                     TANYLDRNMLLWTAASGTTGTLIKTQNIYAGNYSFLVTTGDRVWGRTIDLNKSPELKG
                     KFVWFGAWVNDQGSASKLMFIIN"
BASE COUNT         3425 a         2791 c         2980 g         2994 t
ORIGIN      
        1 catccaaaca gagtcacagc ctgagaatga aagccaggat gacaacggaa aagagatggc
       61 aactgatcct gaaaaaaccg aagagacacc agaagattac gccttgcgta ttggtgatga
      121 agaaattcag cttaacgctg acgataatga tcacattgac gggcaacctg caccgcaatg
      181 ggtgaaagat cttcgcaaag gcttcaaaga aacacagaaa gaaaaccgtg agttgcgacg
      241 ccagcttgag gaagcattag ccaagcctgc ggaacatcag caaccacaac cagacgctat
      301 tccaccaaaa ccgactcttg agtcgtgtga ttatgacgaa caggcgtttg aacaggcatt
      361 gactgattgg catgagaaaa aaggccgtgt cgaacagcag cagcaacaaa aactacgtca
      421 gcaacaggaa taccagcagc gtttccagca aagggtagaa gcgcataaac aacgggcagc
      481 caaacttcct gtgaaagatt atcaggaaat ggaagccatt gttcttagtg agctaccacc
      541 aattcagcag gaaatcatca ttcactgtgc agacgaaggc tctgaactac tcgcctatgg
      601 cttaggtaag agccagcaat tacgccagcg tgtagccgct gagacagatc caattcgcgc
      661 agcattcctc ttggggcaga ttagcaaaca ggtaagcctt gctccaaaac caaagaaagc
      721 catcaagcca gagccggaag tacgtggtgg cggtgctgat gcgaaacaag acgaattcaa
      781 caaattatgc cccggcgcaa aaatcgaata aggaaaagat aaatgcctaa caatctcgac
      841 agtaacgtca gtcaaatcgt tctgaaaaaa ttccttccgg gttttatgtc agatttagtt
      901 ctggcgaaaa ccgtagaccg tcagttgctg gcaggtgaaa tcaactccag cactggcgat
      961 agcgttagct ttaaacgtcc gcatcaattc tcatccctcc gtactcccac tggtgatatt
     1021 tcagggcaaa ataaaaacaa cctgatctca ggtaaagcta cggggcgtgt aggtaactac
     1081 atcactgttg ctgttgaata tcagcaactg gaggaagcga tcaagcttaa ccagctggaa
     1141 gaaattctcg cgccggttcg ccagcgaatc gttaccgacc ttgaaacaga gcttgctcac
     1201 ttcatgatga ataacggtgc gttgtcactt ggtagcccca atactccaat caccaaatgg
     1261 tctgatgttg cgcagacggc atctttcctg aaagacctcg gcgttaatga aggtgaaaac
     1321 tatgctgtaa tggatccatg gtctgcacag cgacttgctg atgcgcagac tggtttgcat
     1381 gcttcagatc aattggttcg tactgcatgg gagaacgcac agatcccaac caattttggc
     1441 ggcattcgcg cactgatgtc taatgggctt gcctctcgta cgcagggggc atttggcgga
     1501 acactgacag tcaaaacaca gccaactgtt acctataacg cagttaaaga ctcataccag
     1561 ttcactgtaa cattgaccgg agcgacagcc agcgttacag gttttctgaa agctggtgat
     1621 caggtcaaat tcaccaatac ctactggctg caacagcaga ccaaacaggc gttgtataac
     1681 ggagccacac caattagctt cactgcaacg gttactgctg atgctaattc agacagcagt
     1741 ggcgatgtga cggttacgct ttctggtgtt ccgatttatg acactacaaa cccgcagtac
     1801 aactctgtaa gtcgtcaggt agaggcaggc gatgccgtat ctgtagtagg cactgctagc
     1861 cagacaatga agccaaacct gttctataac aagttcttct gtggactcgg atctatccca
     1921 ctgccgaaac tgcacagtat tgattctgct gttgcaacat atgaaggttt ctccatccgc
     1981 gtacataaat acgcagatgg cgatgccaac gtgcaaaaaa tgcgcttcga cttactgcct
     2041 gcatatgtgt gctttaaccc acacatgggc ggtcagttct tcggtaatcc gtaataacaa
     2101 ggggcttacg ccccttttat gttttaagga aacaatatgg atcggatgag tgtattcctt
     2161 gccgcagata acgaatccgg gcatgtacag gccgttatcg cagaaaaaga cttccagttt
     2221 ttcgaaaggt tgggctttgt tgcctcagtt gatgaattga aaccgaccag taagcgaggt
     2281 cgtaaggcgg cggacaatgg caacagtact gacaaagggt gagatcgtcc tttttgcgct
     2341 tcgtaagttt gctattgctt ctaatgcatc gcttactgat gttgagccgc aatcaattga
     2401 agatggtgta aatgatctgg aagatatgat gtccgagtgg atgattaacc ccggcgacat
     2461 tggttacgct ttcgcaactg gagatgagca gccattacca gatgatgagt caggtcttcc
     2521 aagaaaatac aaacacgcag taggctatca gttattgctg agaatgctat ctgattacag
     2581 ccttgaacca actccgcaag ttctcagtaa cgcccaacgc tcatatgatg ccttgatgac
     2641 cgacactctg gttgttcctt caatgcgacg acgtggagat tttcctgtag ggcagggtaa
     2701 taaatatgac gtgttcacat ctgaccgata ttatccaggc gatctccctc tgattgatgg
     2761 cgatatccca aacgcatagg tgaataaatg cctattcagc aacttccgct tatgaaaggt
     2821 gtcggcaaag actttagaaa cgccgactat atcgactatc tgccagtgaa tatgttggct
     2881 acacccaaag aaatcctgaa cagcagcgga tatcttcgct cattcccggg cattgccaaa
     2941 cgttctgatg tgaacggcgt atcgcgaggc gtcgagtaca acatggcaca gaatgctgtt
     3001 tatcgcgtgt gtggtggcaa actgtataag ggcgaaagtg aggtcggtga tgttgccgga
     3061 agtggtcgcg tatcaatggc gcatggtcga acatcacagg cggtaggcgt taacgggcaa
     3121 ctggtcgaat accgctatga tggcacggtt aaaaccgtct caaactggcc tgcagacagc
     3181 gggtttatgc agtatgagtt aggttctgtt cgcgacatta cgcgtttacg tgggcgttat
     3241 gcgtggtcaa aagacggcac tgattcatgg tttatcactg accctgaaga cgaatcgcat
     3301 cctgaccgct acagcgcaca atatcgagcc gagtctcagc ctgacggcat catcggcatc
     3361 ggaacatggc gagacttcat cgtctgcttt ggttcatcga cgattgaata tttctccctg
     3421 acgggggcaa ccaccgttgg tgccgctttg tatgtcgcac agccatcgct gatggtgcag
     3481 aaaggcattg ccgggactta ctgcaaaacg ccgttcgccg attcgtatgc gttcatcagc
     3541 aatccggcaa caggtgcgcc gtctgtgtat atcatcggct ccggtcaggt atcaccaatc
     3601 gccagcgcga gcattgagaa aattcttcgc tcctacactg ctgatgaact ggctgatggt
     3661 gtgatggaat cgctgcgatt tgatgcgcat gaactgctga ttatccacct tccgcgccac
     3721 gtcctcgtgt acgacgcatc ttcaagcgcc aatggtccgc aatggtgtgt gttgaaaaca
     3781 ggcctgtatg acgatgtgta ccgcgctatc gacttcattt acgaaggaaa tcagataacg
     3841 tgcggcgata agctggaatc ggttaccggg aaattgcagt tcgatatcag cagccagtat
     3901 gggctacagc aagaacacct gctgtttact ccgttgttca aagcggataa cgccagatgc
     3961 tttgatctgg aggtggaatc atcgacgggt gttgctcagt acgctgaccg cctgttcctc
     4021 tctgcaacca ctgacggcat aaattacggg cgtgagcaga tgattgagca gaatgaaccg
     4081 ttcgtttacg acaaacgcgt tttgtggaag cgagtagggc gcatcaggaa aaatgttggc
     4141 ttcaaactgc gcgttatcac taagtcgcct gtcactctgt ctggcgctca gataaggatt
     4201 gagtaatggc ggattcgaat ctcaatgtgc cggtaatcat tcaggctaca cggctcgaca
     4261 catcagtcct tccacgcaat atcttctcgc agtcgtatct gctttacgtt atcgcacagg
     4321 gtactgatgt tggtaatgtg gctaacaagg ccaacgaggc cggacagggc gcttatgacg
     4381 cacaagtcag gaacgatgag caggatgtga ttctcgctga ccatgagcag cgaatttctg
     4441 ctgcggaagc aacgcttgtt aatcatgagg agcgaatcag ccaggcagaa tcaactcttc
     4501 aggaacatga aacgcgaatc gctcagaatg aaagcgatat tgcgtcgctt gataccagag
     4561 ttcagtcgct ggaatcgcag gtttcagacc atgaatcgcg cattgatgct ctggagtatg
     4621 ccactactcg caagaagtca gaggttgttt actctggcgt atctgtaacc atcccgacag
     4681 cgccgaccaa ccttgttagc ctgctgaaaa cgctcacgcc gtcatcaggc tcgttggcac
     4741 cattcttcga caccgttaac aacaagatgg ttgtgttcaa cgagaacaaa accttgttct
     4801 tcaagctgtc gatcgtcggg acgtggccca gcggaaccgc caacaggtca atgcaactaa
     4861 cattttccgg ctctgttcct gacacactgg taagcagtcg caactcggcg acaacgaccg
     4921 ataacatcct gttagctacg ttcttcagcg tggataaaga cggctttctt gccacaaatg
     4981 gcagcacgtt aaccattcag tcaaatggtg cggcgtttac tgccacaacc atcaagataa
     5041 tcgcggagca gtaatgattc agttcaaacc aacgcgaaac atcgacctga tagaagcagt
     5101 cggaaatcac cctgacatta ttgccggaag caacaacggt gatggatacg actacaagcc
     5161 tgaatgccgt tactttgagg ttaacgtgta cggtcagttt ggcggcattg tttactatca
     5221 ggaaattcag ccgcttacat tcgattgcca cgccatgtac ctgccagagg ttcgtggatt
     5281 cagcaaggaa atcgggctgg cgttctggcg atacattctg actaacacca ccgttcagtg
     5341 cgtcacatcg ttcgctgcgc gcaaattccg ccacggtcag atgtactgcg caatgattgg
     5401 ccttaagcgt gtaggaacca tcaagaaata cttcaaaggc gtggatgacg tgacgtttta
     5461 cagcgccaca cgcgaagaac taatcgactt cctgaatcac gggagatagc tatgttatat
     5521 gcatttaagc tgggcagaaa actgcgcggc gaggaacctt attgccctga aaaaggcggg
     5581 aaaggtggca gctctgataa aagcgcaaag tatgcagcag aagctcagaa gtatgccgca
     5641 gacctgcaaa atcagcagtg gcagacgatc atgaaaaacc ttgctccgtt cacgccgctt
     5701 gcggagcagt atgttaacca gcttcagaat ctttccagtt tagaaggtca ggggcaggca
     5761 cttaatcagt attacaactc tcagcagtat aaagaccttg caggtcaggc tcgttaccag
     5821 agtcttgctg ctgcggaggc gacgggtgga cttggttcga cagccacaag caatcaactg
     5881 gctacgatcg cgccgacact cggtcagtct tggttatcaa atcagatgag caattacaac
     5941 aatctggcaa acgttgggct tggtgcactg caaggtcagg caaacgccgg gcagacgtac
     6001 gccaacaaca tgagcagcat tgcacagcaa agcgcagcac ttgccgctgc taatgccaat
     6061 aaaccatcaa gtcttcagac tgcaattagc ggcggcacat ctggtgcgat tgccggtgca
     6121 ggtcttgcca gccttttggg aacatcaacg ccttggggcg ctggcattgg tgctggtatc
     6181 ggattgcttg gctcgttgtt ttaaggggta atcatggcta cttggcaagg aacaaacggc
     6241 ggattgttgg ctggtatcgg cggcgtcaac tcaaacgctc cgagcgtaaa tgacatcggc
     6301 aatacgcttc agcttatcag gcagaacaat gatattgatc gttcaggcgc taacaatgtt
     6361 gggctgactg ctttgcaagg cctttcaggt attgcagggg tgtttcagca ggaaaagcag
     6421 gctcagcggc agaaagaatt tcagcaggcg tacgctaatg cttatgcgtc tggtgatcgc
     6481 ggtgctttgc gtcagttggc tactcaatat ccagaccaga ttgaatccgt tcgtaaaggc
     6541 atgggattca ttgatgaaga ccagcgtaat tctatcggca ccttagcggc tggcgcacgc
     6601 cttgcgtcat cgtctccaga agcaatgcaa tcatggctgc aaaacaacgc caaggaactg
     6661 actcgcgtcg gtgttgaccc taacagcgtt gctcagatgt atcagcagaa cccttcagga
     6721 tttggtgagt ttgttgatca ccttggaatg gctgctcttg gtccaattga ttacttcaat
     6781 gttcaggaca agatggctgg tcgtgaaatt gaccgaggca gactggcaga gacaatccgc
     6841 agcaatcagg ctggagaagc acttcaggcg agagggcaaa acctttccta tcagtcagca
     6901 atgactgggc acaatatcgc agcacaacgc ttggctctgg atcagcaaga gttcgggttt
     6961 aagatgcagc aagcgcagga aaaggctcag cagttgatta gcgaagcacc taagctgtca
     7021 gtaaacatgg aaaaaggcat cgagacggct gtaaacaatg ctacagcatc atcaaactca
     7081 gccaattcta tgagtgcgct tgctcaacag ttcagagcag aaaaaccaac gacaggtttg
     7141 ttcggtaacg cacagaacat gttcgcaaaa cttaccggaa gcgatacaac attgcgtgat
     7201 ttgcgcattc gccaaaatgc ccttgttaac agtcaggttc ttaaattcct acctcccggc
     7261 ccagcaacgg ataaagacgt tgagatcgtt cgacagggtg cgccaactga catggataac
     7321 cctgagacgg tcgcaagatg gcttgatgca atggcaaacc ttgagcgacg aaacgcgcag
     7381 tttaatgagt ttaaagccga gtggatgagc gcgaatggca accctggaca atcgcgtaat
     7441 ggcggtcaga tattggggtt ggatgttaaa aaaggtgaat cattggggag tgccgttaag
     7501 cggtatatgt caatgaatac tgacgcagcg ccagcacaag attcgacacc ttcaggagaa
     7561 ccacggaatc aggttggatc atatacctca aaatcaggca ttcaatttac ggtggaatga
     7621 tgaaagtaac tgcaaacggt aagacattta cctttcctga tggtacgagc accgaagata
     7681 ttggcaccgc cattgatgag tattttgctg gtcaggctgt tcagcaacaa acagttaatc
     7741 aggccaataa tgcaccaaca cgggacgaac catcattgat gcaacaagct ggcgattggc
     7801 tcactggtgg tcaaagtgca gggcaaattg cagaacaggc tggtcgtggt ctggtaaaca
     7861 taccatttga cgtattgcag ggtggcgcaa gtctgattaa tgcaatcagt caggggcttg
     7921 gtgggccaaa agttttggat gatgtttatc gtccagtaga caaaccgaca gacccatacg
     7981 cacaagccgg tgaaacaatt ggtgggtatt tagttccagg agttggaacg gcaggaagca
     8041 tggctattgg atcgctggca gaggccgcaa atcagaaagg cgatttcgca caaaatgcag
     8101 ctaaaaatgc cggagttaac cttgccgctc agggtgttct ttccgcagca gcaaagggaa
     8161 tagggcgtgg aataacggct ataaaaggtg atattgcgcc agaagtggcg aagaaaattg
     8221 ccacatcaga atcgatgggc gtgacaccaa tgacatctga tgttatcccg ccgaaaaatg
     8281 ctttcactcg cggccttact caggatgccg agggggcttt gctcgggacg ggctcaaagc
     8341 gagcagagca atatgcaacg cgtagtaagc tggtaagcaa ttattttgac cgttttggtg
     8401 agtacaaccc tgatgatgtg gtgaaatctc tgaccaccac gttaaggggg cggaaggatg
     8461 ctgctggcgc tgttatcaat gacgtcacca ataaaatggg taatgccgca gttgatacta
     8521 caaataccat gaatgctctg aatacagcga tcgcaagaca ggaacggctt gggacttcag
     8581 ccaatcaaag cctgcttaca tccttgcgta acctgcgtga agaattagca aaccctgcaa
     8641 ctgatttgga tgttacgttt gatctcttgc gtcagcacag aacagcattt agatctaatg
     8701 ttcagggaga tgctatggtc ttccccaacc aggcaaaagc agctaccaat atggtagaga
     8761 atgcaatgtc aaaagacctt cgtaacgcag ttgcaaaaaa cctcggtgcg tcagacgcag
     8821 caaaatacct taaagcaaat tccgattatg caaacgttta taataaggtg cttaataaaa
     8881 acattgctaa caagctcaac aaggcaagca gtgaagccag tcctgaactt ataaatactg
     8941 ttgtattaag cagaaaacca tctgacgtga aacgaatctg gagcgcattg gatgataaag
     9001 ggaaagatgc tatgcgtgca gcttacgtca gcaaaatagc ggaaaaggcc ggtgactctc
     9061 cagccaagtt catcactgaa gttaataagc tgaaatctca gtcaggtggt gaaatttaca
     9121 acactatttt ttctggaaag cacatgaaag agcttgattc tcttcatgaa gttctacaga
     9181 aaacagcaag gtcagacacc gcaaatgtag taactcagac tgggcaatcg caagccaaca
     9241 ggataaggac gattggcgca actgcgactc ttggcgtatc aatggggctt gaggctggtt
     9301 tcggtgcaat gatgcgcttg tatgagtcca aagcagcaag gaatgctctc ttacgtttgg
     9361 caaacaccaa agcaggaaca ccagcctatg aaagagcgct aaataatgct gcaaatgcga
     9421 tacgccctat actctcaagc caaattacag cagaacagca ataaaagata agatgtaact
     9481 acctgatatt actgctactg ttgcatgtta ccgtgtttcc aaatcctgaa ttgcagtttg
     9541 tatatgtgtc aacgcgtgtt gggtaaggtt gagttataac aggctggcgc gctttttgct
     9601 cgatcgcttg cattgtgttt acagcctgat aattcaataa agcctgctgg aatgcttggc
     9661 tttgtgctat ttgttgggct tgttcttggc tttgtaattg aacataaaga ttctgaagct
     9721 caagtcttgc ctgtgcgtca cttatcttgc cttcatcgac acctttcccg agcatctttg
     9781 cagcaaggac atacagctta ggtgttggtg ctgatgccat gcgtgagtcg ttcttcacac
     9841 tggcatcaag gcaattagcc atatcgctaa gcttttgata gcgttgttcg caacttgctt
     9901 gatagtcact tacttttgcg catccaacca gcagaagcgg gataattaac agtgattttt
     9961 tcatatggtt aactctcctt agtttttcac aggatatcat gaaggcaatg ccattttagc
    10021 cggaaactag atttctatgt ttccttttta ttattgctat acatggtctt aagcgtttca
    10081 aaaaccattt tcttaaccat atcagattgt tgttctgcca tacgctctgc atcatcaatg
    10141 taaacggatg cagagctttg tttagccaac gattcttcaa tcgctgcaat tatctctgag
    10201 ttcagcgacc tgttattcat cttcgcgcgc tgtttaattt tcgcgtggag ttcatgcgga
    10261 agtctcaagt gaaactgcgc ctcgtcgtat ttgctgtaca tccttgatgc ctcaccagtt
    10321 gggtggaatg gcatcgtaac ctactggata aatactcaat agtaccattt cggtatgcaa
    10381 tcacatcatg gttgcatcat atcattcttc tggagcaatg aaatgtcaga tatcaccgca
    10441 aatgttgtgg tcagcatgcc ttcgcaactc ttcactatgt ctcgctcttt taaagccgta
    10501 gccaatggaa aaatttatat cggtaaaatt gacactgacc cggtaaatcc agaaaaccag
    10561 attcaggttt atgtggaaaa cgaagacggt tctcacgttc ctgtttcgca accaatcatc
    10621 attaacgctg ctggttaccc tgtatataac ggacagattg ccaagttcgt aactgtgcaa
    10681 ggccattcta tggctgttta tgatgcatac ggtgcgcagc agttctattt tccgaatgtg
    10741 ctaaagtatg atccagatca gtttaagggc aatctcctga gcgaagatca ctcacttggc
    10801 gactctctag tcatgcataa cccacagttt gtcgatagca catcgaccat tctctcaaaa
    10861 aagctaacta tcgacctgag gacgcttaca gactacgggt ttgctgaggg aaacaccggt
    10921 gctcagaaca aagctgcatt tcagaaggct attgatgatg ctacgttgcc aactgagatt
    10981 gttataccag agggggcgtt cattgttgac cctggtatta cgattaagaa tactgtcacc
    11041 atgatacgag gtgctggcgc atatcagtcc agaattttct ctacgggtac agcggcaccg
    11101 ataatcacgc aacaagatgg ggtaattacg ttctgcgagt tcagggactt tggtcttgac
    11161 ggaaatggct acgcagctaa cggcatcagt cttactgaag ccaaccacat taaaatagaa
    11221 aatattgacg tggttaatac taataacaac gcaatattag ttaacggtta ctccattgac
    11281 atcattgggt gccggttgtt tcaaaacgcc ggtaacggta ttaatgttgg tggttactgc
    11341 aataatatta atataatcaa cagccgtatc tatggtaacg gagccggtgg agttttactt
    11401 acgccagctt acgctgaggg cggtatgagc gtcagagtta atggcaacaa tatagagcaa
    11461 aataagttct acggactttt ggcttacggg gttaaaggcc ttaaccttga tgctaactat
    11521 tgggaacgca acggtgagat tggttacccg tatagtgtac ctgagtctat tacagtacgt
    11581 gcggatatcc accttatcgc gaacaatttt acattaattc ctgacctttc aaaaattaat
    11641 gataccgtat ctattcgcgg gaatcagcag accgcgattg gctacgccag cgcgctacct
    11701 aaccaagacg gatttatttt cacgaactat gcgaaaaacc tgacgattga aaataatcag
    11761 ttattagacg catcgaaagt gaataattta ttagcaatgt accacaataa tctttcgtcc
    11821 aaagttactg acaggctata tcttgccaac aacacagtga actccatcgg atacgttggt
    11881 agttatgacc cggcgacaca aaaccctgac actgcgcatt taatagatat tgcgaacaga
    11941 gagttaactg ctaactatct tgatcgaaat atgttgctct ggactgcggc atctggaact
    12001 acaggaacat tgataaaaac tcaaaacatc tatgctggta attattcatt cttagttacg
    12061 acaggagata gagtttgggg acgaacgata gacctcaaca aatcaccaga actaaaaggg
    12121 aaatttgtat ggtttggtgc gtgggtcaat gaccagggat cagcgtcaaa acttatgttt
    12181 ataattaatg
//
LOCUS       DAZGLJ010000134        12188 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000134 DAZGLJ010000000
VERSION     DAZGLJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.134"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(55..963)
                     /gene="galU"
                     /locus_tag="ACHK49_003265"
     CDS             complement(55..963)
                     /gene="galU"
                     /locus_tag="ACHK49_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO9263872.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(1165..2178)
                     /gene="rssB"
                     /locus_tag="ACHK49_003266"
     CDS             complement(1165..2178)
                     /gene="rssB"
                     /locus_tag="ACHK49_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO9263873.1"
                     /translation="MTQPLVGKQILIVEDEPVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(2270..3175)
                     /gene="rssA"
                     /locus_tag="ACHK49_003267"
     CDS             complement(2270..3175)
                     /gene="rssA"
                     /locus_tag="ACHK49_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO9263874.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            3288..3746
                     /locus_tag="ACHK49_003268"
     CDS             3288..3746
                     /locus_tag="ACHK49_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO9263875.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            3796..4638
                     /gene="purU"
                     /locus_tag="ACHK49_003269"
     CDS             3796..4638
                     /gene="purU"
                     /locus_tag="ACHK49_003269"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO9263876.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     SDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            4798..4882
                     /locus_tag="ACHK49_003270"
     tRNA            4798..4882
                     /locus_tag="ACHK49_003270"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4832..4834,aa:Tyr,seq:gta)
     gene            4930..5061
                     /locus_tag="ACHK49_003271"
     ncRNA           4930..5061
                     /ncRNA_class="other"
                     /locus_tag="ACHK49_003271"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5245..5922)
                     /gene="narI"
                     /locus_tag="ACHK49_003272"
     CDS             complement(5245..5922)
                     /gene="narI"
                     /locus_tag="ACHK49_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO9263877.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(5922..6632)
                     /gene="narJ"
                     /locus_tag="ACHK49_003273"
     CDS             complement(5922..6632)
                     /gene="narJ"
                     /locus_tag="ACHK49_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO9263878.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(6629..8167)
                     /gene="narH"
                     /locus_tag="ACHK49_003274"
     CDS             complement(6629..8167)
                     /gene="narH"
                     /locus_tag="ACHK49_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO9263879.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(8164..11907)
                     /locus_tag="ACHK49_003275"
     CDS             complement(8164..11907)
                     /locus_tag="ACHK49_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO9263880.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         2910 a         3296 c         2991 g         2991 t
ORIGIN      
        1 aaaaaacggc gtcgattgct caacgccgtt tcgtggataa caccgatacg gatgttactt
       61 cttaatgccc atctcttctt caagccaggc tttaaattcc gtgccaaggg tgttatgacg
      121 aataccgtat tcaacgaagg cctgcatgta acctaattta ttaccgcagt catggctctt
      181 ccctttcata tgataggctt ccaccgtttc tttttcaatc agcatatcaa ttgcgtcggt
      241 gagctgaatt tcatcaccag ctcccggagg ggtttttgcc agcaacggcc aaatatccgc
      301 gctaagtacg taacgaccca caatagcgag attagacggc gcaacatccg ctttcggttt
      361 ttctaccaca ccaaccatcg gtacgctttc acccggcgct aattcaacgc ctttgcaatc
      421 cacaacgcca tatgcggtca catcagcaac cggttcaacc atgatctggc tatgacccgt
      481 ttcatcaaag cggcggatca tctctgccag gttatcctgt gacaaatcgg attcatattc
      541 atccagaata acatcaggca aaataacagc taccggttca tcacccacta ccgggtgagc
      601 acacaatacc gcgtgtccca ggcctttcgc cagaccctga cgaacttgca taatagtcac
      661 gtgcggtgga caaatagact gcacttcatc aagcagttga cgttttacac gtttttccag
      721 cattgcttcc agttcaaaac tggtatcaaa gtggttttca atagagtttt tagatgagtg
      781 tgtaaccagc acaatttcag taatgccagc cgcaatacat tcattcacga cgtattgaat
      841 taatggctta tcgacaagtg gcagcatctc tttcgggatg gctttcgtcg ccggcaacat
      901 cctggttcct aatcccgcaa cggggataac ggcttttttg actttcgtat taatggcagc
      961 catttaaatt ctcctggact gttcgtgttt tgaacgtgtt catatttctg tatcgcatcc
     1021 cagtatatca gcaccccgaa ggggctaagt tctgaaaagg aggcgagtta gcataattaa
     1081 gacaattact tatacgaatg gcgacgatag taccaccagc cgacattagc aggtaatgca
     1141 aatttagccc gcgttatcgt ttgctcattc tgcagacaac atcaagcgca gtcgaccacc
     1201 ggttccccat atttggcatt gccaggcatc gcatcgctgg ctcaattgat tcaaataagc
     1261 gttacctaaa gtgcctaacg gaacaccatt actgatttgc acctggtgtt cgccggtatt
     1321 taacgtcgca ttcagacccg cagaaaccag aatgagattt ttcagttcgc gatgataata
     1381 gccaactaat agcggaaact gccccggcag attggcctga cgaagtaaat ggtttacctg
     1441 cttcaataac gcgcccaact ctggcaaccg ttgattttgg tgtgcaagct gttcctgtaa
     1501 taatccgtta aacaatgcgc gtaataacaa ggcagcaagt acgccattat gtccagctcg
     1561 ggtgacatca aggcaataaa atgccagatc gttttccgaa agtgcggcaa tatcaagcac
     1621 caggccgggt ttgtccgcgg caaccaattg acgataatta acccggcaat gggaaatcac
     1681 ctgctgaacc ggcggttgta gttcctgtaa taatttcgcc gctgcggcag ggttatcaac
     1741 cattgcatcc cagtcgcgaa aaagcctttc ctcttcctca acgcgcgaat taaacatgct
     1801 gggatagaga caggcaaaaa ccatctcgcg caagcgattc aaatctttaa ctggtttcag
     1861 caaaacatct tcaacgccca gacgtaacgc tttggcaata tctgccatat tttcggtggc
     1921 agatatcacc agaaccggtg tctggtcacc tctgttgcgt atatgttcca gcagtttaag
     1981 cccgttcatt cgtggcatcg cgatatcaca tatcatcagg tctggagtga aacctcccag
     2041 caactcaagg gcatccaccc catcagccgc cagtaccgtt gtcgctccca atgaggaaaa
     2101 ccacgaatcc agaagcgagc gaaataccgg ctcatcttca acaatgagaa tctgttttcc
     2161 gaccaatggc tgcgtcatgt tctctcccct gactggcttt actcaatagt ggcatgctat
     2221 tgccacctgc gcctgtcaga attaacttaa gtgtagataa aaattctggt caaatgttgg
     2281 tgcgtaccaa cggcaaaagt tcgtccattt tcttttccac tgccagctgt ccggccgcaa
     2341 tggcagcgtg cgcgcgatgg aaatcaagcg tagatatttg cgggcaaaca ggttgaatca
     2401 gaatatcggg cggatcacct gccatgcggt tccttttaag gcggttctcc agcacctgga
     2461 tagaagtggt cataatctct gttgccgttg gcgctgtcac cgcccgacgt gtcgttatgc
     2521 tgcctaacct ttctttcaga cgcgcatgcc acggcagaga atcaccattc tcgctatttt
     2581 cttcactgac attaaaggag agcaaatctt gttgcatcaa atgagcatcg tgctgcaggt
     2641 caaccgctat cacaatatca gcccccaatg cacgcgtgag ggaaatagga attgggttaa
     2701 cgactgctcc atcaaccagc cagtagccgt tatgtgcaac aggtgccatg agtcctggaa
     2761 tactgcatga tgcgcgaata gcaagatgga gatcgccttc agtaaaccat aattcacgtc
     2821 ccgtacttaa attggtggca acagccgcaa agcgacggga acaattttcg atctctgttt
     2881 ccggcattat ttcgcggtat tgattgaaga cacgctcgcc gcgcagtaac ccaccgcgct
     2941 gccaggagag atccatcagg cgtaaaacat cccaataact gaaagaggtc acccaatctt
     3001 ccagcgcaga taatcgatcg catgcatagg cagcgcccac cagcgaacca attgaacatc
     3061 ctgcaacgat atcaatttca atacccactt tttttagcgc attaataacg ccaatatgcg
     3121 accaacctct cgccgcgcca gatcccagcg ccagccctat ttttatcttt ctcattattc
     3181 ccgcaagatt cccctggaat gcaatcgtag ccacatcgct actgctcggt taacatagtg
     3241 ctactcaggc ggttaatacg ccgttattgt ttccagggag attatttgtg tctcagcttt
     3301 gtccctgtgg tagtgctgtc gagtatagcc tatgttgcca cccttatgtg tctggtgaaa
     3361 aggttgcacc tgatccagaa catctcatgc gttcgcgtta ctgcgctttt gtgatgcaag
     3421 acgcagatta tttaataaag acctggcatc cctcttgtgg agcagcggcg ttacgtgccg
     3481 aattgatggc cggatttgcg catactgaat ggttgggatt gaccgtattt gaacattgtt
     3541 ggcaggacgc cgacaatatc ggatttgtca gttttgttgc tcgatttacc gaaggtggta
     3601 aaacaggtgc aataattgaa cgttcgcgat ttttgaaaga aaacggtcaa tggtactata
     3661 ttgacggtac acgtccgcag tttggtcgca acgatccctg cccttgtggt tcaggtaaaa
     3721 aatttaaaaa gtgctgcggc caataatggt tgacggtacg gtttagcaaa cactctcaac
     3781 aaggtttttc cagcaatgca ttcactccaa cgtaaagttc tgcgtactat ttgtccggac
     3841 caaaaaggtc tgatcgcacg tattaccaat atttgctaca agcacgagtt aaatatcgta
     3901 cagaacaatg aatttgttga tcaccgtacc gggcgctttt ttatgcgcac ggaactggaa
     3961 gggattttta atgattccac cctgctggcg gatctcgata gcgcattgcc agaaggctcc
     4021 gtgcgtgagc tgaatcctgc cggtcgtcgc cggatagtga ttctggtcac taaagaagcg
     4081 cattgccttg gcgatttgtt gatgaaagcc aattacggcg gcctggatgt cgaaatcgcg
     4141 gcagttattg gtaaccacga tactttacgt tctctggttg agcgttttga tattccgttt
     4201 gagctggtaa gccatgaagg gttaagccgc aacgagcacg atcaaaagat ggcggacgcc
     4261 attgatgctt atcaatctga ctatgtggtg ctggcgaagt atatgcgggt attaacgccg
     4321 gaatttgtgg cacgcttccc gaataaaatc atcaatattc accattcatt cctgcctgcg
     4381 tttatcggcg cacgtcctta tcaccaggcc tatgaacgtg gtgtgaagat tattggcgca
     4441 accgctcact atgtgaatga caatctggac gaaggcccaa tcatcatgca ggacgttatt
     4501 catgtcgatc atacctacac agctgaagat atgatgcgcg caggtcgtga cgtcgagaaa
     4561 aacgtcttaa gtcgtgcgct atacaaagta ctggcacagc gcgtctttgt ttacggtaat
     4621 cggacgatta ttctttaatc gccagcaaaa ataactggtt acctttaatc cgttacggat
     4681 gaaaattacg caaccagttc atttttctca acgtaacact ttacagcggc gcgtcatttg
     4741 atatgatgcg ccccgcttcc cgataaggga gcaggccagt aaaaagcatt accccgtggt
     4801 ggggttcccg agcggccaaa gggagcagac tgtaaatctg ccgtcatcga cttcgaaggt
     4861 tcgaatcctt cccccaccac catcactttt caaaagtccg taaactctca aacgaatccg
     4921 caattaaata ttctgcccaa gcggggaagg atgagaagct tcgaccaagg ttcgacttga
     4981 gcgccagcga gagagcgttg ccgcaggcaa cgacccgaag ggcgaagcgc gcagcgttga
     5041 gtaatccttc ccccaccacc atcacttcct taaataatca aaacctgtta ctccgagcta
     5101 attttgcccg atggcaccac gtttaccggg cctacaatat cctcccctgt aggtcggata
     5161 agcgaagcgc atccgacaac tcaccccgca ccgtgcccat aaaaaaaccc cgccgaagca
     5221 gggtctatgt gaactaaaat tcgcttagtg acgagcgcgc accagctggt acttacgtgt
     5281 cagatactcc accggtacgc tccagatgtg taccagacgc gagaacggga acagcaggaa
     5341 taacgtcatc cccagcacca ggtgcagacg gaagataaac gccacaccat cgaggtgttg
     5401 agaagcgcca ccgtggaagg tcaccaccga ctgcgcccag ccaaccagtt tcatcatctc
     5461 gctaccgtcc atatgctgag cggagaacgg aatggtcaac aggcccagcg cgcactggat
     5521 aacgagcagc gacaggatca ggatatccgc tccggtagtg gttgcacgca cgcgtgggct
     5581 gaacagacga cgcttcagca acagcacgcc gccaatcaga cacagcacgc cgctggcacc
     5641 accagcaaac atcgccattt tctgtttcac ttcaatcggc agccaggctt catacatcca
     5701 gtgcggcgtc agcataccga agaagtgacc aacaaaaatc cccagaatac cgatatggaa
     5761 caggttcgac gccaggttca tccctttgcg atccagcatc tggctggacg ccgcgcgcca
     5821 ggtgtactgc ccgtagtcat aacgcagcca gctaccaatc aggaagaccg ccccggctat
     5881 gtacgggtag atatcaaaga agaacatatt caggaattgc attagtgctg tcctccggtg
     5941 gtgatattca gatattgcgg cgcgacggca ccggcaaagc gacgctgatg agcagtgatt
     6001 gctgaatcac cgcagccttt gtcagcaaag aatttaacct gctcttcttc ccagacagca
     6061 tccagcgcct gcggcgtatc atcgcgcgct tcgtcggcaa ttttttccgc cactttgtcg
     6121 ctgtcgatag cggtattggc caatttcagc agcagatcaa acatcacggc ataacggctt
     6181 tcacgctgtt gcagacgcgc gctcagcaat gccagaatcg gcgcgatatc tttcaaacct
     6241 tccacggctt cgctttgcgg cagctgcgac aggtactcca gatacagcgg cagatggtcc
     6301 ggtaattcgc ggctgtttaa ctgcaagccg tgctgctcgt actgcgccag caggtccacc
     6361 atcgcctggc cgcggtcacg ggattcaccg tgaacatgtt caaatagcaa cagtgacgtg
     6421 gcgcggccac ggtcgaacag ttcgctgtac tgggcctggg catcgagcgg atccatcgcc
     6481 gttaaatcgc gcaggaaaat gcccagcgca tgggcatctt ctttcgacag atttttcgac
     6541 gcagcaatcg cctcaaacat ctcttgttga tgctgccata aggcagcatc cggatattca
     6601 aggagacgcg atacaatcac gagttcgatc atggatgcgg ctccgttttg ctggtcacat
     6661 cgatggcatc gatacgacgg ctgttgaaca gattgaattt ggtatctgaa ccgtggcagc
     6721 catcaccaaa ggtaaagccg cagccatttt tctccgggaa ggcttcccgt gccagttcac
     6781 gatgactact cggcaccaca aagcgatctt cgtagttagc aatcgccaga taacggtaca
     6841 tctcctgtgc ctgggcttcg gtcagaccga cctcttccag cgcacgggta tcaactttac
     6901 cgtcaacggt ttcagcacgt ttgtaatgac gcatcgccaa catacgtttc agtgcgcgca
     6961 gtaccggttt ggtatcaccg gcggtcagca gattcgccag atactgtacc ggaatacgca
     7021 agctttcgac gtctggcaga atgccgttgc tacccagctc acccgcgtct gctgcagact
     7081 gaatcggaga cagaggcggc acgtaccaga ccatcggcag tgtgcgatat tctggatgca
     7141 gcggcagcgc cagtttccat tccattgcca ttttataaac cggcgactgc tgtgcggctt
     7201 caataacgct cagcggaata ccgtctttaa tcgcctgctc gatgactttc ggatcgttcg
     7261 gatcgaggaa cacctccagc tgacgctggt aaagatcttt ctcgttctcg gtgctggctg
     7321 cacgttcaat cgcgtcggca tcgtacagca gcacgccaag ataacggata cgaccgacac
     7381 aggtttctga gcacacggtc ggctgacccg cttcaatacg cggatagcag aagatgcact
     7441 tctcagactt accgctcttc cagttgaagt agattttttt gtacgggcat ccggtgatgc
     7501 acatacgcca gccacggcat ttatcctggt cgatcaggac gatgccatct tcttcacgct
     7561 tgtaaatcgc accgctcggg caggtcgcca cacatgccgg gttcaggcag tgttcgcaca
     7621 ggcgcggcaa atacatcata aaggtgtttt cgaactggct atacatcgcc ttctggatgt
     7681 tgtcgaagtt cttgtctttc gccagtttgt caaactcacc acccaggtca tcttcccagt
     7741 tcggcccttt ttcgattttc gccatccgtt ccccggtaat cagcgaacgc ggacgggcaa
     7801 tcggctgcga tttgctgcct tccggcgcgg tatgcaggtt ctgatagtca aaatcgaacg
     7861 gctcgtaata atcgtcgatc cccggcagat gcgggttagc gaagatttta cccagcaaca
     7921 tggcacggtt acccatgcgc ggctgcagtt tgccgttgat tttacggatc cagccgcctt
     7981 tgtatttttc ctggttttcc cagtcagtcg ggaagccctg acccggcttg gtttccacgt
     8041 tgttgaacca cgcgtattcc acgccttcac ggctggtcca gacgttttta caggtaactg
     8101 aacaggtgtg gcagccgatg cacttatcga gattcagcac catgccgact tgtgaacgaa
     8161 ttttcatttt acgctctcct gtacctggtc attgccttcg ccatctaacc agtcaatgtt
     8221 cttcatctta cgcactacaa caaactcatc gcggttagaa cctacggtgc cgtagtagtt
     8281 aaagccgtat gccagatggg catagccgcc gatcatatgc gtcggtttcg gcgtgatacg
     8341 ggtgaccgag ttatggatac caccacgctg ttgggtaatt tccgaaccag gcaggttaac
     8401 gatacgttcc tgcgcgtggt acatcatggt catccctgcc ggaacacgct ggctgacaac
     8461 cgcacgggca gtcagagcac cgttgctgtt gaagacttca atccagtcgt tatcggcgat
     8521 acccagatct ttggcatcgg cttcactcaa ccagaccacc ggaccaccgc gacctaaagt
     8581 cagcatcagc aggttgtcgc tgtaggtgga gtggataccc cacttctggt gcggcgtcag
     8641 gaagttgagc gctttttccg ggttgccatt ggatttctgg cccatcactt ctttcaccga
     8701 acgggtgtcg atcggcggac gataaaccag caggctttca ccgaaatcac gcatccattg
     8761 gtgatcctga tacagctgct gacggccaga gagcgtacgc cacgggatca gctcgtgaac
     8821 gttggtgtaa ccggcgttgt aagaaacgtg ttcatcttca aggccagacc aggtcgggct
     8881 ggagatgatt ttgcgcggct gtgcctgaat atcgcggaag cgaatcttct cgtcttcttt
     8941 attcagcgcc agatgcgtat ggtcacgacc ggtaaactcg ctcagggcag cccaggcttt
     9001 cacggctacc tgaccgttgg tttccggtgc cagcgtcagg atcatctctg ccgcatcaat
     9061 tgcggtgttc agcatcggct ggcctttcgc cggaccttcc gctttggtgt agttgagctt
     9121 acgcagcaga tccatctcgc tctgggtgtt ccaggcaatc cctttaccgc cattaccgat
     9181 tttctccatc agcgggccga tagaggtaaa gcgttcgtaa gtcgccggat aatcgcgctc
     9241 tacgaccata atgtgtggcg cggttttacc agggatcagg tcgcactcgc cttttttcca
     9301 gtctttcaca tccagcggct gcgccagttc agcggcagag tcgtgctgga taggcagcgt
     9361 gacgatgtcg gtttctttac ccagatgacc gacgcacact tcggagaatt tcttcgcgat
     9421 ggctttgtaa atttcccagt cgcttttcgc ttcccaggcc ggatcgaccg ccgcagacag
     9481 cgggtgaata aacggatgca tatccgaagt attcatgtcg tctttttcgt accaggtcgc
     9541 cgtcggcaaa atgatgtcgg aatagagaca ggtgctcgac agacggaagt ccagcgtaac
     9601 caccagatcc agcttgcctt ccagaccatt gtcctgccag tccacttctt ccggcttcac
     9661 gccgccctgt tgccccagat ctttaccctg gataccgtgc tccgtcccca gcaggtactt
     9721 gagcataaac tcatgacctt taccggaaga accgagcagg ttagaacgcc agatgaacag
     9781 gttgcgcggg tggtttttac cgttttctgg ttgttctgcc gcaaaacgga tggaaccctc
     9841 tttcagggat ttcaccgtat agtccaccgg attcatcccg gctttttccg cttcgccagc
     9901 gatagtcagc gggttagtgc ctaactgcgg tgcagacggc agccagccca tgcgttccgc
     9961 acggacgtta aagtcgatca agtgtccggt atagcgggat ttgtccgcca tcggtgacag
    10021 caactcttcc gccgtgacgg tttcataacg ccactggctg gagtggttat agaaataaga
    10081 agtgctgttc atgtgacgcg ccggacgctg ccagtcaagg gcaaacgcca gcggctgcca
    10141 gccggtttgc ggacgcagtt tttcctgacc tacatagtgc gcccagccgc ccccgctctg
    10201 accgacacag ccgcagaaaa tcagcatgtt gatcagacca cgatagttca tatcgaggtg
    10261 ataccagtgg ttcagccccg caccgacgat aatcatcgaa cgaccgtgcg ttttatcagc
    10321 gttatcggca aattcacggg cgatgcgaat aatctggctg cgagaaacgc cggtaatctg
    10381 ctcggcccag gccggggtat aagctttcac atcgtcatag ctggttgcac agttaacgtc
    10441 gttcaggcca cgttccagac cgtagtttgc cagcgtcaga tcataaacgg tggtcaccag
    10501 ggcggtgctg ccatcagcca gttgcaggcg tttcaccggc agtttgtgca gcagcacgtt
    10561 ttccagttcc actttgttga agtgttccgt gccgtcgcca ccaaagtacg ggaagcctac
    10621 ctcagcgatc tcatcctggc tacccagcag gctcagttgc agctcggttt cttcgccagt
    10681 tttgccgtcg cgctgctcaa gattccattt gcccttctcg ccccagcgga agccaataga
    10741 accgttcggc gcaaccattt cgccattggt attaaaggcg acagttttcc attccggatt
    10801 gttttcctgg cccagcgcat caaccagatc agcagcgcgc agcatacgac ctgcagcgta
    10861 gtaaccgtcg cgttcttcca gcatcaccag catcggcatg tcggtgtagc gacgcacata
    10921 gtcggtgaaa tactggcttg ggttgtcgag gtggaattca cgcagcatta cgtggcccat
    10981 cgccagcgcc attgccgcat cggtgccctg tttcggtgcc agccacagat cgcacagttt
    11041 ggcgatttca gcgtagtctg gtgtgacggc aacagttttg gtccctttgt aacgcacttc
    11101 agtaaagaag tgagcatccg gggtacgcgt ctgcggtacg tttgaccccc aggcgatgat
    11161 gtagctggag ttgtaccagt cagcagattc cggtacgtca gtttgctcgc cccaggtttg
    11221 cggagacgca ggaggcaagt cgcagtacca gtcgtagaag cttaagcaag taccgccaat
    11281 cagcgagaga tagcgtgcac ccgatgcgta agaaaccatc gacattgccg gaattggcga
    11341 gaaaccagca acacggtccg ggccgtagtt tttgatggtg taaacgttag atgcggcgat
    11401 cagttcgttc acctcctgcc aggaagaacg aacaaatcca ccgcgtccac gcgcctgctt
    11461 aaagcttttc gctttatcgg cgtcttcaat gatagaagcc catgcctcaa ccggatcgct
    11521 atgcagcgcc ttcgcttcac gccacatttt catcaggcgt ttgcgcatca tcgggtattt
    11581 caggcggttg gcactgtaaa gataccagga gtagctggca ccgcgcgggc agccgcgagg
    11641 ttcatggttt ggcagatccg gacgggtacg cggatagtca gtctgctggg tttcccaggt
    11701 gaccagaccg tttttgacgt agattttcca gctgcaggag ccggtgcagt ttaccccgtg
    11761 ggtagagcgg acgattttgt catgctgcca acgctggcga tatccatcct cccagtcacg
    11821 gttggtattg agaagctggc catgcccatc ggcaaaggtt tcacccttct gtttgaagta
    11881 gcgaaaccgg tccaggaatt tactcatcgg ttttctcctg tgggagcctg tcggccctct
    11941 gataaatcga cattgctaat gtttaagggc aaggtaacgc tctgaaacag cgtgggaatt
    12001 gataacgatc aagtgtgatg gggatgaaaa ataaagtaaa ttcctaagat acccctttag
    12061 gataattaac agttaaagca taacatgctg taaattaaga cgttttatac ctgtagttat
    12121 ttaaagcatg aagattttta tgcagatggg tattaaggag tattccccat gggtaacata
    12181 ttaatggc
//
LOCUS       DAZGLJ010000135        12132 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000135 DAZGLJ010000000
VERSION     DAZGLJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.135"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(29..181)
                     /locus_tag="ACHK49_003276"
     CDS             complement(29..181)
                     /locus_tag="ACHK49_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9263881.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(446..1180)
                     /gene="cysH"
                     /locus_tag="ACHK49_003277"
     CDS             complement(446..1180)
                     /gene="cysH"
                     /locus_tag="ACHK49_003277"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO9263882.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1254..2966)
                     /gene="cysI"
                     /locus_tag="ACHK49_003278"
     CDS             complement(1254..2966)
                     /gene="cysI"
                     /locus_tag="ACHK49_003278"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO9263883.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDHEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2966..4765)
                     /gene="cysJ"
                     /locus_tag="ACHK49_003279"
     CDS             complement(2966..4765)
                     /gene="cysJ"
                     /locus_tag="ACHK49_003279"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO9263884.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5084..5446
                     /gene="queD"
                     /locus_tag="ACHK49_003280"
     CDS             5084..5446
                     /gene="queD"
                     /locus_tag="ACHK49_003280"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO9263885.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5524..6795
                     /gene="ygcN"
                     /locus_tag="ACHK49_003281"
     CDS             5524..6795
                     /gene="ygcN"
                     /locus_tag="ACHK49_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO9263886.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLEPSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6786..7046
                     /gene="ygcO"
                     /locus_tag="ACHK49_003282"
     CDS             6786..7046
                     /gene="ygcO"
                     /locus_tag="ACHK49_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO9263887.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCCLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7063..7638
                     /gene="ygcP"
                     /locus_tag="ACHK49_003283"
     CDS             7063..7638
                     /gene="ygcP"
                     /locus_tag="ACHK49_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO9263888.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7786..8646)
                     /gene="ygcQ"
                     /locus_tag="ACHK49_003284"
     CDS             complement(7786..8646)
                     /gene="ygcQ"
                     /locus_tag="ACHK49_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO9263889.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAVKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8643..9422)
                     /gene="ygcR"
                     /locus_tag="ACHK49_003285"
     CDS             complement(8643..9422)
                     /gene="ygcR"
                     /locus_tag="ACHK49_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO9263890.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRIEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9400..10809)
                     /gene="ygcS"
                     /locus_tag="ACHK49_003286"
     CDS             complement(9400..10809)
                     /gene="ygcS"
                     /locus_tag="ACHK49_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9263891.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10831..>12132)
                     /locus_tag="ACHK49_003287"
     CDS             complement(10831..>12132)
                     /locus_tag="ACHK49_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO9263892.1"
                     /translation="AAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVE
                     NSVVLDGSAMNQIINIDIENMQATAQCGVPQEVLENALREKGYTTGHSPQSKPLAQMG
                     GLVATRSIGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGTDIRHIIIGNEG
                     ALCYITEVTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDG
                     TQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLN
                     WGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGG
                     HSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIG
                     KHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
BASE COUNT         2746 a         3356 c         3131 g         2899 t
ORIGIN      
        1 tacgcggggt tttacccccg cgtggtggct acttcttcga ttcgtaagcg agaactgcct
       61 taaactccat accacgttct ctgatactca actcacacaa cgagtcacct accatcagcg
      121 tgaatcccag tactgtacaa cacagtacga tgattgccac aagggcatat tttgtcagca
      181 tgtcttgcct ccctgcaaag aagagactaa cattcgaatt gtttgggttc gaacgtaagc
      241 ctcaggttga tagaaatatc gcctggggct tttgtccatc tggaacctcg cgaatgctta
      301 acgccagaca gcctcaagca cccgacgcca ttctatacct gataattctt ccagcggcgt
      361 tcacgtttaa aatcaggaat actgctcgca aaataaaaac gcccccatca tttctgacag
      421 aggcgtttaa tttatccggc aatatttacc cttcgtgcaa tccacattcc ctttttaagc
      481 caaagaaacg tgtttcttct tccgacatgc caggttccca tttacgggtt gtatgggtat
      541 cgcccaccga taaatatcct tcgtcccata atgggtgata tttcaggcca tgtttttgca
      601 gatactgata tatcgttcgg ttatcccagt cgataatcgg cagcacttta aatacgccac
      661 gctgaattgc cagcaccggt aaattggcgc ggctgccgga ttgttcgcgg cgcaggccag
      721 caaaccacgt ttgcgcattc agttctttca gcgcccggtt catcggttcg actttgttga
      781 tgtcattgta cttttcaatg ccttcaacgc cctgttccca cagtttgccg tagcgtgctt
      841 cctgccaggc ggcgctttcg gtagcgcggt acactttcag gttgagcttg agtttgtccg
      901 ttaactcgtc aataaagcgg taggtttccg ggaacagata gccggtgtcg gtgaggatca
      961 ccggaatatc cgggtgaatc tgattcacca gatgcaggct caccgccgcc tgaatgccaa
     1021 agctggaaga aagtacatat tcaccgggca gattatccag cgcccaggca acgcggcctt
     1081 cagcgtccag tttttccagt tcggcgttag tttctgccag cgccagaatg cgatctacct
     1141 tcggcagttc gttcagggcg tttagatcga gtttggacat agattcctca ctgtttgcct
     1201 tgcctgatgc gacgctcacg cgtcttatca ggcctacaag accgggctga tggttaatcc
     1261 cacaaatcgc gcgccggatc gagcaccggg cgaatgatgc ccgcacgcac cgtaaagtcg
     1321 ccgaagcctt cacccgcttc gcgctctttc gcccagcgcc ctatcagttc atcaagcgac
     1381 gccaggattt ccggctcggt gatgttttct ttatacatcc gtgggatacg tgtcccaatg
     1441 cggttgccgc caagatgcag gttgtagcga cccggcgctt tacccaccag gcccacttcc
     1501 gccagcatcg cgcgaccaca accgttcggg cagcctgtta cacgcatcac gatatgctca
     1561 tcgctgacac catgtttcgc cattaaatta tcgatgttgt cgataaaaga cggcaggaaa
     1621 cgctctgctt ccgccatcgc cagcgggcaa gtcgggaacg agacgcaggc catcgagttt
     1681 tcacgctgcg gcgtgacggc attcattaac ccgctctctt tggcgatctt ctcgatcttc
     1741 gctttctcgc tttccggtac accggcgatg atcagattct ggttcgccgt aatgcggaaa
     1801 tcgcctttgt ggatcttcgc gatctccagc aggccggttt tcagcggacg ccccggataa
     1861 tcaaggatgc gaccattttc gataaacagc gtcaggtgcc agttatcatc aatgccctta
     1921 acccagccaa tacgatcgcc tcgtccggtg aactcatatg gacggatcgg ttcaaatttg
     1981 atccccgcgc gacgctccac ttccgcttta aacgtctcaa cccccacgcg ctccagcgtg
     2041 tatttggttt tggcattttt acgatcggtt cggttacccc agtcacgctg agttgtcacg
     2101 acggcttcgg ccaccgccag cgtatgctcc agcggcagat agccaaactc gctcgccgtg
     2161 cgggcgtagg ttttcttgtt gccgtgttcg atagaaagcc caccgcccac cagcaggttg
     2221 aagcctacca gcttgccgtt ttcggcgatt gccacgaagt tcatgtcgtt ggcatgcaga
     2281 tcgatatcgt tctgcggcgg gatcactacc gtggttttga acttacgcgg caggtaggtc
     2341 tgaccaagga tcggttcttc atcagtagtg gcgacttttt cgtggtcgag ccagatctcc
     2401 gcatacgcgc gggtacgagg caacagatgc tcagaaatct tcttcgccca ctcgtacgct
     2461 tccgcgtgca gctgcgactc gtaagggttc gaggtgcaga gtacgttacg gttcatgtcg
     2521 ttagctgtcg ccagcgcatc aagaccgacc gagtgcagca tctggtgcac cggtttgacg
     2581 ttctttttca gaatgccgtg gaactgaaac gtctggcggt tggtcaggcg aatgctgcca
     2641 tagatggtgt tttcaccggc aaatttgtcg atcgcctgcc actgtttagt ggtaatcacc
     2701 ccacccggca gacgacagcg aagcagcatc gcgtggcgcg gctccagctt ctgttcagca
     2761 cgttcggcgc ggatgtcgcg gtcatcctgc tgatacatgc cgtggaagcg gatcagcagg
     2821 aagttgtcgc ccttaaagcc gccggtcaga ccgtcgttta aatcttccgc aatggtgccg
     2881 cgcaggtagt tgctttcaag cttcatgcgc tcggcgtctg tcagttttcc ttcgaccact
     2941 aaaggccctg gatgtttttc gctcattagt agacatctcg ctgataacgg cgctctacgc
     3001 gcagctcact taaaaattca tccgccgctt cggtgtccat gccaccaaat tcggcaatca
     3061 cttccagaag tgcctgctca acgtctttcg ccatgcgatt agcgtcgccg cagacataaa
     3121 tgtgggcacc atcattgatc cagcgccaca gctccgcgcc ctgttcgcgc agtttgtctt
     3181 gtacgtaaac tttttctttt tggtcgcgcg accaggcaag atcgatacgt gtcagcacgc
     3241 catctttgac gtagcgctgc cactccacct gatacaggaa gtcttccgta aagtgcggat
     3301 taccaaagaa cagccagttt ttacctggcg cttcgtcggc ggcgcgttgc tgcataaagg
     3361 cgcggaacgg cgcaataccg gtgcctgggc caatcataat caccggggtt tccgggttag
     3421 cgggcaggcg aaagttatcg ttatgttcga taaatacgcg gacttcgccc tcttcttcca
     3481 cgcgatccgc gaggaagctg gaggcaccac cggcacgggc tcggccttcc acgtcgtaac
     3541 gcaccacacc aacggtgacg tgtacttcgt tctcgacttc cgcctgcgag gaggcgatgg
     3601 aatacaggcg cggggtcagc gggcgcagca gattaatcag cgcttcggca tccagttgcg
     3661 ccggagagaa acgcaccatg tcgacaatcg gcgtcgtcgc ggcgtaatgc tgtaacttcg
     3721 ctttatcgcc caccagcggc agcagtgttt cgctgcgggt aagcgtggcg taattctcaa
     3781 caatgttggc ggtgttgacg gtcagttcga agtgccactg tagcgcttcg ttcagaggca
     3841 acgttttgcc ctcgacggtg acaggttcat cgcctttcag ccacagcagt tcgacaagtt
     3901 ctttcaccag tgccggatcg ttctgatacc agacgcccag cgcgtcaccc ggctggtaac
     3961 gcaggcccga gtcacctaag tcaatttcga tatggcgaac gtctttttca gagttacgcc
     4021 cggtaatttt ctggttaaca gagaggctag ccaccagcgg cgcgtctttg ctgtacgggc
     4081 tggtgtggat ttcatttacc gcgccagtag cgacggattg cgaaggtgcc gcgacaggcg
     4141 cacgcgattt aagcgcatca accacgcggg cgcgccactc gctggcagca gcctggtatt
     4201 caacatcggc atcgacacgg tcgagcaggc gttcaccacc cagttccgcc agcttgctgt
     4261 cgaaatcttt cccggactgg cagaaaaatt cataagagct atcgccgagg ctaaacacgg
     4321 caaacgcggt gttttccagc tttggcgctt ttttggagaa caggaactta tgcagcgcga
     4381 cggcttcttc cggcggttcc ccttcccctt gcgttgacgt cactacgatg agcagttttt
     4441 cgctggcgat ttgtttgaat ttatagtcgc ccgcgttcac cagcttaacg ttcagttttg
     4501 ctgctaataa atcatcgcgt aatgcttcag caacccggcg cgcattgccg gtttgcgagg
     4561 cggagataat agttataccc ggcatttctg cggctggcgc tggcgtcgct gcaagcgcag
     4621 caggctgctg attgagcacg ccccagaaat agccagaaac ccaggcaagc tgggtgggag
     4681 ttaaatcggt cgtggccgcc tgaaggcgtg ccagttgctc cgggttcaac ggaagcaacg
     4741 cggaaggtgg gacctgtgtc gtcatgcgtc gttatgttcc agtaagcaaa gctgtttctg
     4801 cgccctgtca gcgcccataa aacagaagag aaggtaaggt taacggggtg aacggtgtgg
     4861 attaaagacg ggatagcgat aactaataac caaatcgact aacctgtttt agcaatagtc
     4921 tttaacaaca aaatagatta accaacctaa tgaaaaacaa atgaatttag ccaatcatta
     4981 agataaatca gcgattttgc gcaacaaagt cgttttagat aatgcgaaaa aacagccttt
     5041 ccggtactct acggcggttt tatcgtcctg tagagaaatt atgatgtcca ccacgttatt
     5101 taaagatttc accttcgaag ccgctcaccg cttaccacac gtcccggaag ggcataaatg
     5161 tggtcgcctg cacgggcatt cctttatggt gcgactggaa attaccgggg aagtcgatcc
     5221 gcatacgggc tggattatcg atttcgctga actaaaagcg gcgtttaaac caacctacga
     5281 gcgcctcgat caccattatc tcaatgatat tccaggtctg gaaaacccaa ccagcgaggt
     5341 tttagcaaaa tggatttggg atcaggttaa acccgttgtg ccgctgttaa gtgcggtgat
     5401 ggtaaaagaa acctgtaccg caggttgtat ctatcgcggc gaatgataag agtgtgtcgg
     5461 cggtcaattt cccttaagta acgctatgtt agggtgttgt gttctggata tctggggcat
     5521 gacatggaag acgactgcga cattattatt attggtgccg gtattgcagg caccgcttgc
     5581 gcgttacgct gcgcgcgagc gggtttatcc gttttgttac tggaacgcgc tgaaatcccc
     5641 ggcagcaaaa atctttccgg cgggcggtta tatacccatg cactcgcgga actcctcccg
     5701 caatttcatc tgaccgcgcc tcttgaacga cgcatcactc acgaaagcct ttccctgtta
     5761 acgccggatg gcgcaacgac gttttccagc ttacagcccg gcggtgaatc ctggagtgta
     5821 ttacgtgcac ggttcgatcc gtggctggtt gccgaagccg aagaagaagg tgtcgaatgc
     5881 atccccggag cgacggtgga tgcactgtat gaagaaaacg gcagggtgtg tggtgtcatt
     5941 tgtggtgacg atattctccg tgcccgttat gtggtgctgg cagaaggtgc caacagcgtc
     6001 ctggctgagc gtcacgggtt agtgactcgt cctgctggtg aagcgatggc gttggggatc
     6061 aaagaagtgc tgtcgctgga accatccgct attgaagaac gttttcatct ggagaataac
     6121 gaaggcgcag cgttgctgtt cagcggcagg atctgtgatg acttacccgg cggcgcattt
     6181 ctttatacta atcaacaaac gctctcgtta gggattgttt gcccgctctc ttcccttacg
     6241 caaagtcgtg ttccggcaag cgagctgctg actcgcttta aagcgcatcc ggcagtgcgc
     6301 ccgcttatca aaaacacgga atcactggag tatggtgcgc atctggtgcc agaaggtggc
     6361 ttgcacagta tgccggtgca atacgccggt aacggctggc tgctggtggg cgatgcgttg
     6421 cgcagttgcg tcaataccgg aatttccgtg cgcggcatgg atatggcgct gactggcgcg
     6481 caggcggcgg cacaaacgct gataagcgcc tgccagcacc gcgagccgca aaatctgttt
     6541 gcgctttatc atcacaacgt agagcgcagc ctgctatggg atgttctaca acgttatcag
     6601 catgttccgg cgcttttgca acgccctggc tggtatcggg cgtggcctgc gttaatgcag
     6661 gatatttccc gcgatttatg ggatcagggt gataaacctg ttccaccgct gcgccagtta
     6721 ttctggcgtc atttacgtcg tcatggcctg tggcatctgg cgggcgatgt tatcaggagt
     6781 ctgcgatgtc tgtagcccat aatctctggc gcgttgctga tgcgccgcac attgttccgg
     6841 ctgactccgt tgagcgtcag atggtacaac ggttgattaa cgcctgtccg gcaggtcttt
     6901 tttcgctcac accggaaggt gacttacgcg ttgattatca cggctgcctg gagtgtggca
     6961 cctgctgttt gctgtgcgac gaatccacac tacaacagtg gcgctatccg ccttccggat
     7021 tcggcatcac ctaccgcttt ggataaataa taaggataat ttatgcccct cttacacctg
     7081 ctccgccaga atccggtgat tgctgccgtt aaagataatg ccagcctgca actggcaatc
     7141 gactctgaat gccagtttat ttccgtgttg tacggcaata tctgcaccat cagtaatatc
     7201 gttaaaaaga ttaagaacgc cgggaaatat gcttttattc atgttgattt gctggaaggc
     7261 gcgtcaaata aagaagtcgt gattcagttt ttgaaactgg tgaccgaggc ggacggcatt
     7321 atcagtacca aagcctcaat gttgaaagcg gcaagagcgg aaggtttttt ctgtattcat
     7381 cgcctgttta ttgttgattc aatttcgttt cacaacattg ataagcaagt tgcgcaatcg
     7441 aatccggatt gtattgagat cctgccaggc tgtatgccca aagtgctggg ctgggtgaca
     7501 gagaaaatcc gccaaccgct gattgccggt gggctggtgt gcgatgaaga agatgcgcgt
     7561 aatgcgatta acgcgggtgt cgtggcgctt tccaccacga ataccggggt ctggacgtta
     7621 gcgaaaaaat tactttgacg ggataatcgt aaccaattga atttggtttg atttgtaggc
     7681 cgcacgccac atccgacatt cagcgcctga tgcgacgctt gacgcgtctt atcaggccta
     7741 caagtcccca ggccagaacc gtaggcagga tcatccggca acacgttatt ggcaatctgc
     7801 gtgaatgttt gtcaccagtg cctccagcac cactttccag tcatcaacca cgccaacatc
     7861 tgcctgtgaa aataccgcag cgctggcgtc atgattaatc gccaccacaa acttgctatt
     7921 gcgtacgccc gccattaacg ctgctgcgcc agaagcgcca acaacaatac acacctcagg
     7981 tgccagcaga tgtccggaaa taccaatcac tttttcagca tcaacgccac cgttcatcac
     8041 tcgcgcccga ctgtagccca cttcggctcc cagcttttct gccagcatag cgatttcctg
     8101 gttatctgcc tcaccgccct gccccaccac cagcacccgt ctggcctcag ctaatgggtc
     8161 acgggtaaca tttttcagat cctctgtgct aaccagccag tcgggaagcg cgccggggac
     8221 aatgttcagt tgctgcatac cagagggcaa cgtggcgttt ttcactgctc ccgcctgacg
     8281 cgccagggaa aggcatagcg ggcgtttttc tgtttgcagc gtcgccgtta gcgcatttcc
     8341 ccagtgggat ttgcgcacgc ttacggttgg aatatctaac gaagtcacct ggcagatgct
     8401 tgcaccatgt aaacgccagg ccagccgggt cgacagttca tcgccaaacg ttcccggcgg
     8461 aaagagaaca acgtccgcag gcgttcgctg ccactgttcc accaacgcat ccagcacctg
     8521 ttccgccact acaggctgag gttcaatctg ccagtgcgcc agggtacaac cgctgaaatc
     8581 ctgcgccgcc agccagcttg ctattgcggc attctcctga tttattgtca caattgcgat
     8641 attcatggct gcatcctttg gcgtaaatag tcctgccata gcttttgagc tttttctgct
     8701 accgtttgcc catcaatcag cgtcgctccg cgtcgttgct ctgctcttgc cagttgcaga
     8761 cactgcatag ctggcatttc ggcagcaaca gttttacgga taatttctgc tttccccgca
     8821 gccattcgct ggcgcattcc cggtactggc aaggccactt caccgcactg acgcacggca
     8881 atcaccgcag gcaagcgaac ccggcaacaa cgcaaaccat gttcaatacg ctgttcgagg
     8941 gtgataaaca gcgcgtcgag agtgaaacgc tccacctggg tgaagcaggg ccagcccagc
     9001 atttccgcca gcaaaaatgg cgtttgccca ttctgccctt cgctgctttg gcagccggta
     9061 attatcagat ccagcggatt ctgatgctgc cattcggcaa tatggcgagc gacaaattcc
     9121 ggcgcaaagc gcagatctgc cgccgtctcc agcaatacag cttcctcaaa cccaagagcc
     9181 atgagatagc gcagccagtg caatgcccgt tcatccccca tgctcaacgc ggttaaagac
     9241 atcggcgtac cgttttttct ctgcgccaac agcagcgcgg cagcggcctg ttcatcagca
     9301 ccgagtaaac ttcgcagtag cgaaatatcc ggtccgcttt taccctgagc cgccgcctgc
     9361 cactcttttt ccgccagcat tccggcatcc ggttcggctt taaacgctaa cagaatgttc
     9421 attcgcacct cctacatttc ccgccgccac cagcgggagg gctttagttt ctggtgccca
     9481 tagccaggtc acaacaaaac caaccaacaa cactgtcgcc aggagcaata aggtgacttg
     9541 cattccccac tgcgccagca cccacggcag caggccagta cttaccgccg cgccaagtcg
     9601 actcatggcg gtggcaaaac cgacgcccag cgagcgaatg tcagtaggaa aactttccgc
     9661 aggcaaaatg cccaccagat tactgactgc cgaaatggtg gtgctgaaga gaacaaaaag
     9721 cagcagcgtt aatgaactgc cggaaggcaa acaggccatt actaccagcg ttgccgccag
     9781 cagcaaaaaa cttcccagca aaaatttgcg atgtgccagc aggtgcgtca gaactaatcc
     9841 cagcagcgcg cccacaatta acaacgcatt aagcatcagg ctggcagtca gcgcatcttc
     9901 cagaccaata gtctgggcga tagttggcag ccaggtataa atcacaaacc atgggattac
     9961 gaggcagaca aagaagacgc tgttaaacgc cgtgcgccgc cagtaacgcg aagagaacaa
    10021 ggttttgatg tgtttatggg tcgccgttac cacttcatcg cccagtaaaa catggggacc
    10081 aaaatagcga tgcacgatag cgtgagcttc tgcaaaacgc ccctggcgca gtagccagcg
    10141 tggtgattct ggcgttcccc agcgtaataa cgtaatcaac aacgcgggca gagctgccga
    10201 tgccagtagc cagcgccagg cctccgggtt ttcggaaata aagtgatgtc cggcaatact
    10261 tgccagcaca tagcctacgg tccacaccac gctgaatgcg cccagcaaaa taccgcgatg
    10321 gcggcgcggg gaaaattcag ccagcaaggt gtgacctact gaataatcgc ctcccagacc
    10381 aatgccaatc aaaatgcgca gtccaataag atgctctggc gtggtggcaa aaaattgtaa
    10441 aaacgaagca agcgtaatca gcaaaaagct gaaggtgaag attttttgcc gaccaatatg
    10501 gtcggagatc cacccaagaa ccaggctgcc aaggaacaaa ccaaggagcg ccgagccgcc
    10561 gatcattccc gccataaacg gcgtcagttg catggcgggc gtaagctgaa taatggcgta
    10621 accaatgacg ccgagaacat aaccgtcggt caggtgtgcg ccgaaagtga gcgcagcaat
    10681 gcggcagtga aaacggttaa gcggtaaatc atccattcgc accggtgaag tgttcatctt
    10741 taccccttca ttgtccggta ataagaacgg cttctcgcct gagaagccga aaaggcaacg
    10801 ccccgtcatc gcggaacgct gcctgacaca ttatttttca atcggataga tagtacccgt
    10861 attcataatg ccattaggat cgaactgctt tttcagccct tccagcaacg cccacgcgct
    10921 gccgtgttcc agtttgctcc agtgaacgcg atgtttaccg ataccgtggt ggtgcaccat
    10981 cgaaccgccg aggcgaatgg tttcttcaca gatgatcttg ttgagcggat tgtggtactt
    11041 gtcgatttcc tcttccggct tacagtcaac gacgttgtaa tcgtagacga agtacatgtt
    11101 ggtgccgttc tgatagctat gagaggaatg accgcccagc atggtgatgt cgtcggcgtg
    11161 cgggaactca gtacgaatac ggttaataac gctttcgtag atttcgtgga tgcagctcca
    11221 gcagccggac acttcagtgg taaagcccat gttgccggtt ttgaggatct gcacacgttc
    11281 ggcagccact ttatccggtc cccagttcag gttgttaaac caggtttcga tcagtttgct
    11341 gtccacgcgc tggcactgcg ggtagcgggc aacgatttcc gcaatccctt cgcccgtcgc
    11401 cttcgcaatg cgagggttac cttcagccat aaagatcagc acacattttc cgtctgcaaa
    11461 atgggtgaag tgttgggtgc catcttcagc gtcatacaaa cgagcgatcg acggacgata
    11521 cccttccacc atgacttcac gcaggatgtt gaagccggtt ttcatgtctt ccaggatata
    11581 gccgtagaag aggttgtttt ccggggtgaa tttaaagatt ttcactgtta cttcagtgat
    11641 atagcaaagt gcaccttcgt taccgatgat gatgtggcga atgtccgtgc cagccgcgcg
    11701 gcgtggcacg tttttaatgc gtgtgacggt gccatcagcc aatactgctt ccagaccaac
    11761 gaccatatct tcgattgcgc cgtagagtgt ggagaactgc ccgatactgc gggttgctac
    11821 caggccgccc atctgcgcca gcggctttga ctgcggagaa tgccccgtgg tgtaaccttt
    11881 ttcacgcaac gcattttcca gcacttcctg gggaacgcca cattgcgccg tcgcctgcat
    11941 attctcaata tcaatattaa tgatttgatt catggcggaa ccgtcgagca ccaccgagtt
    12001 ttctacaaca gtttccagcc caccttcagt ggcggaagcg ccggtacgcg gcacaccgtt
    12061 aattttgtgc gcattcataa aattcagcac acgggagact tgttctgtgg aaccgagttt
    12121 tacgaccgct gc
//
LOCUS       DAZGLJ010000136        11949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000136 DAZGLJ010000000
VERSION     DAZGLJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.136"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            237..1550
                     /gene="gltP"
                     /locus_tag="ACHK49_003288"
     CDS             237..1550
                     /gene="gltP"
                     /locus_tag="ACHK49_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO9263893.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(1628..2317)
                     /gene="yjcO"
                     /locus_tag="ACHK49_003289"
     CDS             complement(1628..2317)
                     /gene="yjcO"
                     /locus_tag="ACHK49_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO9263894.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(2411..4558)
                     /gene="fdhF"
                     /locus_tag="ACHK49_003290"
     CDS             complement(2411..4558)
                     /gene="fdhF"
                     /locus_tag="ACHK49_003290"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4139..4141),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO9263895.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(4756..6222)
                     /gene="mdtP"
                     /locus_tag="ACHK49_003291"
     CDS             complement(4756..6222)
                     /gene="mdtP"
                     /locus_tag="ACHK49_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO9263896.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALLQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVALFAGLDLDLWGV
                     HRSAVAAAIGAHNATLAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFQGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(6219..8270)
                     /gene="mdtO"
                     /locus_tag="ACHK49_003292"
     CDS             complement(6219..8270)
                     /gene="mdtO"
                     /locus_tag="ACHK49_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO9263897.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATMLEIGSLFLIYKWSYGEP
                     LIRLFIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(8270..9301)
                     /gene="mdtN"
                     /locus_tag="ACHK49_003293"
     CDS             complement(8270..9301)
                     /gene="mdtN"
                     /locus_tag="ACHK49_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO9263898.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(9320..9595)
                     /locus_tag="ACHK49_003294"
     CDS             complement(9320..9595)
                     /locus_tag="ACHK49_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO9263899.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(9804..11789)
                     /gene="yjcS"
                     /locus_tag="ACHK49_003295"
     CDS             complement(9804..11789)
                     /gene="yjcS"
                     /locus_tag="ACHK49_003295"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO9263900.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         2695 a         3127 c         3185 g         2942 t
ORIGIN      
        1 aaatctttcc ataacaaatg gttattcatt aatcctgcca taaactcgcg ttattatgca
       61 ttaatgcagc gaaaagctct gttgttaaag ggttgcgcaa cataccgcgc aaatgatact
      121 gatcataagc gttaaaaaaa tctacaaacc aacgcaacac aattcatgcc ctggcagtat
      181 gtcacgttct cgcgtttctg aacggggaac ggcgctccat tgaggaagtt attctgatga
      241 aaagtataaa attcagcctg gcctggcaga ttctgtttgc tatggtgctg ggcattctcc
      301 tgggaagcta cctgcactac catagcgaca gccgcgactg gctggtcgtc aatttgctct
      361 ctccggcggg tgatatcttc atccatctga tcaaaatgat tgttgtgccg attgtgatct
      421 ccacgctggt ggtgggtatc gcgggtgttg gtgatgccaa acagctcggg cgtattggcg
      481 cgaaaaccat tatttacttc gaggtgatca ccaccgtcgc catcattttg gggatcactc
      541 tggcgaacgt cttccagccc ggtgccgggg tggatatgtc gcagctggcg accgtcgata
      601 tctcgaaata tcagagcact acggaagcgg tacaaagcag ttcccacggc attatgggca
      661 cgattttgtc gctggtgccg acgaacattg tggcgtcgat ggcgaaaggc gaaatgctgc
      721 cgatcatctt cttctcggtg ctgtttggtc tggggctttc ctccctgccc gcgacgcatc
      781 gtgaaccgct ggtgaccgtg ttccgctcca tctctgaaac catgtttaaa gtgactcaca
      841 tggtgatgcg ttatgcaccg gtgggtgtgt ttgcgctgat tgcggtgacg gtggctaact
      901 ttggtttctc ttctctgtgg ccactggcga aactggtgct gctggtgcat ttcgccattc
      961 tgttcttcgc gctggtagtg ctgggaattg tggcgcgtct gtgcgggtta agcgtctgga
     1021 tcctgattcg tattctgaaa gatgagctga ttctggcgta ctccactgcc agctctgaaa
     1081 gcgtactgcc gcgaattatt gagaagatgg aagcctacgg agcaccggcg tcgatcacca
     1141 gcttcgtggt gccgaccggt tactctttta accttgatgg ttcgacgctg tatcaaagta
     1201 ttgccgctat tttcatcgct cagctgtatg gcattgacct gtccatctgg caggaaatca
     1261 ttctggtgct gacgctgatg gtgacctcga aagggattgc tggcgtgccg ggcgtgtcgt
     1321 ttgtggtgct gctggcaacg ctgggtagcg taggtattcc gctggaaggc ctggcgttta
     1381 ttgctggtgt tgaccgtatc ctcgacatgg cgcgtactgc gctgaacgtg gtgggtaatg
     1441 cgctggcggt gctggtgatt gccaagtggg aacacaaatt tgaccgcaaa aaggcgctgg
     1501 cttatgagcg tgaagtgctg ggcaaatttg ataaaactgc ggatcaataa ttgaagattg
     1561 ccggggttat ccacccggca atgtgtggat gcctgatgcg acgctggtgc gtcttatcag
     1621 gcctacctta accgttggtt aatttttcaa actcttcaca cccggtatca aacccttcca
     1681 tacagctcag gttcaaccag tgcagcgcct tttgtttgtt cttctcgata aaaccttcct
     1741 caccgtttaa gaacatcatt cccgcccagt actcggaata accggttcgg gaaattgcag
     1801 agctgcgttt gaaataccag gttgccttgt catcgtcggc cttaatgccc acgccgttgg
     1861 cgtaaatcaa accaagcagc atttgggcat cgacggcgga gtcattctcc agatcttccg
     1921 aggcgttttc taacagcgaa atcgcctttg gataatccgg tttacccgct tgagtattta
     1981 ccagaatatg cgccagcgtt acttcacctt ctttactgcc cgcttgcgcc gctttctctg
     2041 caagaacttt tgcttgtgga tagtccagac tgaccggatt ggtgattttg atctgcgcca
     2101 gcagcgcgca ggcatcagca tcaccgctgt cggcggcttt ctgtgcccaa tattcggctt
     2161 tgctcaaatc gccggagcta aaccagctgt cggcaagaaa atattgtgcg cgtcggtccc
     2221 cggcctctgc tgcctttaaa tactggctgc caggctcgga gtcgttcgcg tgggcaaaga
     2281 atgtcaaaaa caacatcaat gcgataattt ttttcatttt gataatacga ttgggtaggg
     2341 agtaaccagt ataaagagtt cgaatggata aaaaaacagc ctccgaaagg aggctgtaga
     2401 aaggactgta ttacgccagt gccgcttcgc gcaggcgagt tttcaacttg ttgtactcgt
     2461 caatcacgta ctgctcggcg gcgcgctgat cggcgatcgg ctcgacgcgc acggcgcagt
     2521 atttgtattc cggcgttttc gtaatcgggc ttaagttttc ggtaaccagc tcgttacagg
     2581 caccaatcca ccactggtag gtcatgtaaa tcgccccttt gttcggacga tcgctgacct
     2641 gcgcacgggt gataattttg cctttacgcg agtgcaccca aaccaatgcc tcatcttcaa
     2701 tacccagacg tttggcgtct tcggtattga tttgtgcgta gccaggttca tcagccagcg
     2761 ccgccagtgc cgcacagtta ccggtcatcg aacggcaaga gtagtgacca acttcacgca
     2821 ccgttgacag taccatcggg tactcgtcgg tgagtttgtc gattggcgct acccagtcgc
     2881 aggtgaagaa ctgtgccaga ccgttcgggg tatcaaactt ctctttaaac agataagaag
     2941 tcccctgatc ggcatctgaa gtatcgcggc aaggccactg aatgaagccc agttcgccca
     3001 ttttctcgta agtcgcaccg tagaaatccg ggcacagatg acgcaactca tcccagatct
     3061 cctgggtgtt gttgtagtgc atcggataac ccatacgggt ggcgatttca ctgatgattt
     3121 gccagtccgt tttcagatcc catttcggtt caactgcttt gaagaaacgc tggaagccac
     3181 ggtcagccgc agtaaacacg ccttcatgct cgccccacga cgttgacggc aaaatgacat
     3241 ccgccgccga cgcggttttg gtcataaaga tgtcctgaac gataaccagt tccagatctt
     3301 caaaggcttt acgtactgcc gacagctccg cgtcagtttg cagcggatct tcgcccataa
     3361 tgtacgcggc acgcacttcg ccatgcgctg cgcggtgcgg cagctcgctg atgcgataac
     3421 cggtatgagc aggcaggctt tccacgcccc aggctttggc gaatttctcg cggttagccg
     3481 gatctttcac gtactggtat cccggatacg tatccggcag cgcgcccata tcgcaggcac
     3541 cctgcacgtt gttctgacca cgaaccgggt ttacacccgc atgcggctta ccgaggttac
     3601 cggtcagcat cgcgaggctg gtcagagaac gcacggtttc cacgccctgg tagaactggg
     3661 ttacacccat gccccacagg atggcggcgc ttttcgcctg ggcatacatc cgtgccgcct
     3721 gacgaatctc actggcgctg acgccggtga tatcttcaac cgactccggc gtgtagcctt
     3781 caacgatttt acgatactct tcaaagcctt ctgtacggga agcgacgaac gctttgtcgt
     3841 acagattttc ttcaataatg acatggccca tcgcattcaa cagcgcgatg ttcgagccgt
     3901 ttttcagtgc aatgtgcatg tcagcaatgc gcgcggtttc aattttgcgc ggatcgcaga
     3961 cgataatttt cgccccgtta cgtttagcgt taattacgtg attcgccacg attgggtggg
     4021 aatccgccgg gttgtacccg aaaacgaaca ctaaatcggt attatcaatt tcgttaatag
     4081 cattgctcat tgcgccatta ccgaccgatt ggtgcagacc tgcaaccgat gggccgtgtc
     4141 agacacgagc gcagcagtca acgttattgg taccaataac ggcgcgcgca aatttttgca
     4201 ttacatagtt ggtttcgtta cccgtaccac gcgaggagcc ggtcgtctgg atggcatccg
     4261 gaccgtactt ctctttgatg gcgctcaggc gctcggcaac gtaattcagt gcctcatccc
     4321 aggaaacagg ttcgagtttg ccgccacgct ggcgacggat catgggggtt ttcaggcgcg
     4381 gggtcaggat ctgggtatcg ttaatgaaat cccagccata ataacccttc agacacaggg
     4441 taccctggtt ggttttcccc tgcgctgcct ccgcccggac gattttgccg ttatcgacga
     4501 ccaggttgat tttgcaacct gatgcgcaat aggggcaaac cgtgacgact tttttcatcg
     4561 gtctcgctcc agttaatcaa atcacgcata cgcgctctcg actacagtat gcatctttta
     4621 tgccacattt tatttggggt cattccctga tattacgggc actatttatt caaaactctg
     4681 acgaaaaaca ggctgtcgtc agttttgacg tgacgaaacg aaataccgcg tgacagccat
     4741 cacgcggcag acattttatt ttttctcgac gacgggagcc gcctgatacc cgccgcccag
     4801 cgatttcatc aactgaatgc tttggatcac ccggcggctg tccagcatca gtaatgacat
     4861 ctcttcggca agtactggca accgggcttc agtggcctgt aagcggctgg ttaagccgcg
     4921 ctgataggcg gcctcggcag cgcgctgggt gaagcgcgtt gcttccacgc gttcagcctg
     4981 catttctcgc tcgtcgttga gcgtttgcag acgcgtgccg ttgacggcaa cgtcacgcac
     5041 cgcgttcagt actgactggt tgtaacgttc aatcatcatg ttgctggcgg cgcgcgtgcc
     5101 ttcgagattg gcattcaacc gtccaccgtc aaacagcggc aatttcagac ccgggatgaa
     5161 gttgaactgg cgactggttt ttttgaataa ggtatccagg tggatggcat caaggccgaa
     5221 aaacgctttg atatcaaagc tcgggtagaa cagcgcccgc gcggaatcca cctgatctaa
     5281 tgacgcctga acataccagc gcatggcttg cagatccggg cgtctggcga gcaactcata
     5341 agagagtgtc gccggaatgc cggtctggac tcgtggtaat gccaccggtt tgatctccgg
     5401 catatcgctg gcccccgcgc caatcaatgc acgcagagat tctcgcgttt cggtgatttg
     5461 ccctttgacg gcagcaattt gtttatcgac cgccagaatc tgtgcccgcg cgccctggaa
     5521 aggcacttgt gcttccagac cgtgcgccac tttactctgg tgcgctttca ccgcgtaatc
     5581 aatcacatcg cgagtttgtt ctaacagatc gagcatctga tagctggcct gcatactgta
     5641 ataaagctgc gctacgcccg tggtcagcga tagctctact gctgcggttt ctgccagcgt
     5701 ggcattatgc gcgccaatgg cggcggcaac cgctgagcga tgcacacccc acaaatcaag
     5761 atccagtccg gcaaacaaag ctactgtggc ttccgtatag tacggcccgt ccatacccag
     5821 tgcgggcgca tccatcgcat aagggcttaa aaagccgttc gccgagacgc gttggcggtt
     5881 gagcatccct aacgctgcga cctgtaattg tgaaccggca tctaacaaat cggcctggga
     5941 ctgcgctttt tcttcccgca gtttcgcttc ggcgagggtg tgtgaaccac ttagcgtccg
     6001 ttggagcagc gcatccagct gcgggtcatt gagttgtttc caccactgcg cctgcggcca
     6061 gccggagctg gcaagatgaa tatcgtcggc cagtttgatt tgttccggtt tgagctgttg
     6121 atgaggtgca gaatccttac gtaccagggc acagccggaa atcagcgtcg tgctgccgag
     6181 aatgctgcac aacagcagac gtgaaagttg acgattgatc attgcgtggc tccttgtgcc
     6241 tgttccgtgg cgggcgttaa tgccggggct gtccagtctg gcaagcgggc gaaaagctgg
     6301 cagacgtgtt gctcctgttt taataaggtg ggcaggatgg cctgcgaggc gggtgtctct
     6361 tcaagcgtta tttgaggaga acggctgagt gcggttgcca gaccggcagc gtatttctcc
     6421 agggcgtcgg caaactgagc tgctctgtcc ggctgtagtg cgttatccag cgcctgcgcc
     6481 gagttccagg tggcatccca ggcgtgaaga atatcgcggc cctgacgaat aaccgtttgc
     6541 gaacgttcaa tcagtaaggc gcgttcttcg ctgtccagtt gacgctccag cgccacgcgt
     6601 tggcacatct cttcacaggc attaaacgcc gcatgcagac cgatacgaat ttgcagataa
     6661 gtgcgcagag ccgtgacttc ctgctggcgt gggatccgca ttactttact taacataccc
     6721 agcgtgccag ccagtttttg cggcagtgtg cgcgcttcac tttcaggcca gacaaaggtg
     6781 taaatcaccg cggacaccac ggtaccaatg atgataccca gggcgcgatc gcgaatttcc
     6841 accaggtcgt acactgggcc aaaaacgttt tcgagcgtgg cgagcgcgaa ggtgaccacc
     6901 atctgtgtgc cgatataaga agagcgttca gagctggtgg caatccatgc gcccaacagg
     6961 aaaatcggtg ccagcacaaa cagcaattcg acaatattgt ccagccaggg catgaccagt
     7021 agcgtgaata acagcgccag aatcgcgccg caaaaggccc cgccaaaacg cagcaccatc
     7081 ttctggtacg acgaaccgac atttgggtta gcgacgatca cgcatgtcag catacaggtg
     7141 tgaatgcctt cccagtccac gccgctgtaa aaggtgtaac agatcaaaca ggcgagcagc
     7201 gtttttaccg cgtagcgcat atagtctgga ttggtaaaag catcggcggc cattgatggc
     7261 ggtttggctg cgggcgttgg cggcgtattc gggtccatct gacccagttg taacaacgtc
     7321 tggcagatat tctccaggtt acattcccgt gccgccatcg cttcactttc actgatccgc
     7381 cagtcgcttt gccagcactg accttcagca acggcatgct gcagcttatt gatttctgag
     7441 gctaattttt gccgaaattc aataattgcc tgagaatcag caaaagaggt gggatcgtag
     7501 cgattcagcg tcgagtaaat gtaggttacc gttgccacgc agctttgcca ccatgcgctt
     7561 tgagttcgcc agttggcatc gtccgcgaga caaaagacat tcagtttttg cagcgccagc
     7621 gcctctcttt caatccgcgt ttcgggtagc ggtgcgaggc tgtccgtcag gtggctaatg
     7681 gcatcatcaa gccgatcatt aagcgcctga tgcatttgcg aaatggcacg actgggaaac
     7741 cacagcacgc cgattaacgt catcagcaag gttggataaa ggccaacaac gatacaccac
     7801 agcgttaagc gcacgaccac ttccggatag tcgagcatgg cggggaaggt ttgcccgtaa
     7861 atggcgacaa tggcgactgc gaaaaagacc agtcccagcc gatgggtgcg catcaaaaac
     7921 atacagccca tcaggatcgg cccggcgatg aacaatcgga tcaacggttc gccgtatgac
     7981 catttgtaga tcaaaaacag gctgccgatc tccagcatcg tggcgaccac aaataagatc
     8041 gcgacaaatt tggtgtaaaa cgcgttcgac tgaataccgt aaaacagcac tgccagcgat
     8101 aacgccacga aagggatctc aaaggtcatc gagatcagaa tcaccagcag gcagcctacc
     8161 cagagttgca cggtctgcgg cacgcggcct ggtcgccgct cgcttaactc ttcatgaaag
     8221 aacgccagca gcctgaccac cggtaatggc agggagttga gcgcgctcat tattgcggct
     8281 caagattagc gactgccgaa gcgccgatgc ggaacatttc cgggtcaggt ttatcgacca
     8341 tgattttgac cggaaaacgc tgggcaacgc ggacccagtt aatagagcga gaaactttcg
     8401 gcaggccgcc cagcaccagg ccgccgtcat ccggtagcac gccgtagcca atcgaatcca
     8461 ctttaccctc gaaggttttg ccgctgtcac tcatcaggcg aatcgttgcg ggtgtacctg
     8521 agcgaatatt tttcagatcg gtttcgcgga agttggcgat cacgtaccag tgacgagtgt
     8581 cgattagggt aaaaataggg cgcatggcag aagcaaattg cccgacggag gttttgaggg
     8641 aaatgacccg gccatcaaac ggcgcgcgaa cggtcgccat ttccagatgc agtttggtca
     8701 gggcaatatc cgcttcgacc gccgcacgct gggcaactaa tgcatccacg ccgctgacgg
     8761 cgctggcggc tgactgcgcc tgcaacaata cagcattaag atcggcttct gcagcgcgct
     8821 gcgcggtcct cgcacggtca acgtcttccg ctgagacaaa accttctttc agtaatggct
     8881 cggtgcggcg taatgtatct gtggcctgtt tcgcggcggc acgggctttt tctaccgtgg
     8941 cgttaaccga gtcggcacca aactgttgcg cgtcaacgct acgctgggtg agcataattt
     9001 gcttatccag cgccgcgagg gaggcttcag ctttcgccag attggcttcg tacgggcgcg
     9061 ggtcaatgcg gaacagcaaa tcgccctgtt tgactgcctg gttgtcggtg accgccagtt
     9121 ctacaatgcg accgctgact tccggcacca catcaatggt atctgctgac gcgtaagcgt
     9181 cattagttga tggcgcactg tctacgcgcc agataacgaa aacaagggca accagcgcca
     9241 acgcaaccac taacagagca gggaatttac tgcgaggagc ttttttcggc gtactttcca
     9301 tgagcgcgtc tcaaatagat tagaaaaatg ccagccagaa cagcatggcg tagagagcaa
     9361 aaagggcggt ataaataatt ccgacaaatg ccagattgat atttgcccgc tgaataactc
     9421 gcctcgtgat gagcatcaaa ataaggctgg caatggcgca gaaaaaccag ctgggataat
     9481 aagcaccgat caccgggatt gccggagata atgagcatcc cgaaaccatg aagacgggaa
     9541 ttatccaggc tgtttttttg agttgcatcg ccatgcgaga gagaactgtt ggcatattaa
     9601 atacatcctg tctgctgcga acaatatctg tagtgaatat caatatccat tgaaggcgtc
     9661 agtatggcta ttttctcgat gaataaaaat agctttattc agccaccgca ttcataactt
     9721 atatatttta tttattaatt ggctgtatat atttttaaaa taaatgcttt tgggggcttc
     9781 agcgccccca aaagcgagtg acattaattt ggggttacga tattcaccca caaatcgaaa
     9841 ttatccagac aggcgataat ttcttccagt ttcgcgccat tgccaataat tttggctttc
     9901 ttcgctttta ccagatccgc cattttggct tgcccggtca gcacggcgtg cagatcttca
     9961 cggctgatgt agaaagaggc gtcagcttgg gattgcagtg ttttacggta gttaagcacg
    10021 ctatcgttca gcgtcaggtt gaggttatcg ccattgctca tattgaagtt caggctgata
    10081 tttttacccg cggctttcgc gctatcgagg cgaacggaca taaagtcgaa cagcatttcg
    10141 accgacatcc cgcgaatggt gtccggggaa ccggtggtgc cgtggctgaa cttatgcacc
    10201 ccttcgcgca gctctttcgc gccggtcagg tagaaaccgc gccaggtggc ggactcggcc
    10261 tgatagccca gctgttcaaa gttattcgct tgcagattct tcgcgacctg gtcacccggg
    10321 ttggcggcaa tcacctgttt cagcagttct gccgaccagc ggtaatcacc ttgcttgttc
    10381 gcttcttgcg ccaggttgat gacacgggca gaaccgccca gcgcctgcac gtaacgttta
    10441 cccatctcca cctgaccata cggatgcagg ttagccgggt taccgtcgta atagccaaga
    10501 tagaagttat acaccgcgcg ggcgttgtgg ctgacagaac cgtaatagcc acggctggcc
    10561 cagttattgg caagtgcagg cggcagttta atcatgtcgc cgatttcatt catggtgtag
    10621 ccctggttcg ccaggtgcag ggtctggtcg tgaatgtact tgatagtatc gcgatattta
    10681 ccaatataat cattgatatg cttattgccc cagaccggcc aggtgtgcgg cataaacagg
    10741 acttccgcgt cgttacccca catatccagc gtttcgttca gatactcggt ccacttgctg
    10801 gtgtcgcggg ttttcgcgcc gcgtagagtg tagaagttgt gcagggtatg cgtggcgttc
    10861 tcggcggtac acagcgcttt cagggccgga atatagaagt gcatttcggc tggtgcttcg
    10921 ctacctgggg tcatcaggaa gtcaaactcc aggccgtcga taatcatctt ctcaccggtt
    10981 ttggtgatgg ttttgttcgg tgcgatgatg ctcgggtagc ccgatgccag cgttacgcca
    11041 agaccattgc cgatgttacc ctgagggtta tgcgccagca gcaggccgta ggagtaaagt
    11101 gcacggcggc tcataatatt acccgccagt acgttttcgc tgatggcttc gtccataaag
    11161 ccagcagggg cgataacctg cactttaccg gatttcacat cagcttcaga gataatgcct
    11221 ttcacgccgc catagtggtc ggtatggctg tgggtataaa taaccgcaac aatcggtttt
    11281 tgcgggcgat tttggaagta aaggtcaagg gcggcttttg cgctcggtgg cgtcaccagc
    11341 gggtcgatga caataatccc ggtatcacct tcgataaagg tgatgttgga gatatcctga
    11401 ccgcgaacct ggtacattct gtcggtgact ttaaacaggc cggaaatacc gtttaactgc
    11461 gactgacgcc acaggctagg gttaacggtt tcgggcgcgg gcgcattgat atcaaattta
    11521 taatcttccc cgcgataata tggtttgcca ttagcatcgc gcagaatacc ttcatccagc
    11581 agcggagcga taaatccacg ctgagcgtcg tcaaaatcct gacgatcgct aaacggtaat
    11641 gatttggcgt aattctgatt aacctgttgg gtatattgag tggcggcttt cgcttcctct
    11701 ttcgcgttag cggtatttac catcatgccg ctggcagcag ttaacgcaat aacaatcctg
    11761 ctcaaacgga ataaccgaga gttattcata taaatctcca tttatcattt gtgtcgtaaa
    11821 tatgtaatcc atgcagaata cgtcatttaa agctggcgtt atcataattg ggaatttgcg
    11881 actttaataa gtggaagtgt gagcggaacg atccatttta ttaggcattt gtgctgaagg
    11941 gggaaaatt
//
LOCUS       DAZGLJ010000137        11928 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000137 DAZGLJ010000000
VERSION     DAZGLJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.137"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            24..725
                     /locus_tag="ACHK49_003296"
     CDS             24..725
                     /locus_tag="ACHK49_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9263901.1"
                     /translation="MEALQTTPKTCSLNTYNKVLCDDLDLDSFALTIANLLSAVRTFN
                     LLDDTRLKEVGFDVLEFTHEYALAIASTKQQHSIRSGNKIACIRTKREACGLTTTELA
                     RLLDLDEEIILQWESGEFEPTISMLIPLANVLGCDPLSLLSEKNSESAIRVNTTEVHA
                     ESIGARIKSARTKLGLTESDLARMIHTYSDPINDWECGICEVPADHIVPLASALNCDL
                     MWLLTGKSEAKEQQQ"
     gene            722..922
                     /locus_tag="ACHK49_003297"
     CDS             722..922
                     /locus_tag="ACHK49_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263902.1"
                     /translation="MTGNIHDKYEGLCLAPDSFANNIHNLLCAVIVLQMSDNDAIKRT
                     GDEVLEFARCYAEAAAEKELSS"
     gene            1121..1302
                     /locus_tag="ACHK49_003298"
                     /pseudo
     CDS             1121..1302
                     /locus_tag="ACHK49_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1308..1880
                     /locus_tag="ACHK49_003299"
     CDS             1308..1880
                     /locus_tag="ACHK49_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263903.1"
                     /translation="MQYAEFQAEATANGIQTGSMTIDYHDAIRRLDAGEFDTPNVRGL
                     RILQCLAQADEAGLLGKLPVEMKVAQWRWLYVTTFINEEEDKNGTIDVPNEHGTTDRA
                     VIYNGKHGVMTIYPGPIRFALQQYIEWNLIQKYGEAEGMGRALFLYQKMLITYPDKGF
                     IVSDMGREGLELLLDEMINDLNTHGMPEGQ"
     gene            2065..2253
                     /locus_tag="ACHK49_003300"
     CDS             2065..2253
                     /locus_tag="ACHK49_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263904.1"
                     /translation="MRLYFTIVILLAIIACIYGLLVPFLISMKDTIAVTSGFALAFLT
                     PPCIYAIYKGLSFTKDKK"
     gene            2250..3077
                     /locus_tag="ACHK49_003301"
     CDS             2250..3077
                     /locus_tag="ACHK49_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO9263905.1"
                     /translation="MKKIIFALAIVLPTIGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITATDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGVQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKALAEADAIRLRGEALRQNPGVMELEAINKWNGTLPQYMTSGANTPFIQIK"
     gene            3118..3489
                     /locus_tag="ACHK49_003302"
     CDS             3118..3489
                     /locus_tag="ACHK49_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFO9263906.1"
                     /translation="MNNQNTQPQIMNYDPNLTSCGRMAKQTVRLTFGLWEYRETFEVT
                     VGGNLTGLDVINCAIESLYATLPYEEVLDERTGKTDIMATINIGELICQDEDLSGELW
                     LAGMLISAEIISIEPATNIRL"
     gene            3521..3625
                     /locus_tag="ACHK49_003303"
                     /pseudo
     CDS             3521..3625
                     /locus_tag="ACHK49_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            3681..3935
                     /gene="xisR"
                     /locus_tag="ACHK49_003304"
     CDS             3681..3935
                     /gene="xisR"
                     /locus_tag="ACHK49_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO9263907.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            3969..5255
                     /locus_tag="ACHK49_003305"
     CDS             3969..5255
                     /locus_tag="ACHK49_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9263908.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEIGSN
                     AFSRYQSVVRNMLPRIGEKRLASSVTKEDLLFIRKDLLTGYHNLSNGKTTPIKGRSVV
                     TVNYYMTTIAGMFQFAADNGYIVSNPFNGLTPLKRSRTEPDPLTRDEFIRFIDACHHQ
                     QTKNLWSLAVYTGIRHGELISLAWEDIDLKAKTMTIRRNYTKLGEFTLPKTEAGTDRV
                     IHLVQPAVDALKSQAEMTRLGPQYQIDVKLREFGRTARHECTFVFNPQLVKKCQQVGH
                     HYKADSIRDSWASALRRAGLRHRKAYQSRHTYACWALSAGANPSFIANQMGHANAQMV
                     FNVYGAWMKDNNIGQIELLNKQLTESVPYMPHRARL"
     gene            5239..6036
                     /gene="yecE"
                     /locus_tag="ACHK49_003306"
     CDS             5239..6036
                     /gene="yecE"
                     /locus_tag="ACHK49_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO9263909.1"
                     /translation="MEPDSEIISLNISCFSGSCRHINCVEGNTTLYALPKPEVVLRWR
                     EQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPR
                     ELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHA
                     ACPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQ
                     TTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6089..6484
                     /gene="yecN"
                     /locus_tag="ACHK49_003307"
     CDS             6089..6484
                     /gene="yecN"
                     /locus_tag="ACHK49_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO9263910.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6525..7268
                     /gene="cmoA"
                     /locus_tag="ACHK49_003308"
     CDS             6525..7268
                     /gene="cmoA"
                     /locus_tag="ACHK49_003308"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO9263911.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7265..8236
                     /gene="cmoB"
                     /locus_tag="ACHK49_003309"
     CDS             7265..8236
                     /gene="cmoB"
                     /locus_tag="ACHK49_003309"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO9263912.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8401..10830)
                     /gene="torZ"
                     /locus_tag="ACHK49_003310"
     CDS             complement(8401..10830)
                     /gene="torZ"
                     /locus_tag="ACHK49_003310"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO9263913.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIRVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(10855..>11928)
                     /gene="torY"
                     /locus_tag="ACHK49_003311"
     CDS             complement(10855..>11928)
                     /gene="torY"
                     /locus_tag="ACHK49_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO9263914.1"
                     /translation="LFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHSMSKPFEEYQ
                     GTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDSDDKFETHRQ
                     EMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGETCIDCHKGIA
                     HFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTDLTVVDASGK
                     QPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADEYGNQWRQAS
                     LQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIAKGMGARTSM
                     SENELDILTRYFQYNAKDITEKQ"
BASE COUNT         3134 a         2920 c         2800 g         3074 t
ORIGIN      
        1 acttagaatt gcatggtgat gatatggaag ccttacaaac aacaccaaaa acatgtagcc
       61 tcaatactta caacaaggtt ttatgtgatg atttagatct cgattctttt gcattaacca
      121 tcgcaaacct gctcagtgct gttcgcacct tcaacctcct agatgatacg cgattaaaag
      181 aggttgggtt tgatgtgcta gaatttactc atgaatatgc tttagcgatc gcatcgacaa
      241 aacaacaaca ttctatccga tcaggcaaca agatagcctg catacgcacc aaacgtgaag
      301 cctgcggctt gacgactacc gaactagcca gactactcga tcttgacgaa gagattattc
      361 tgcaatggga gagtggagag ttcgagccaa ccatcagcat gcttatcccc ctggcaaacg
      421 tcctgggatg cgatccgctt tctctactaa gtgaaaaaaa cagcgagtca gctattcgtg
      481 taaatacgac tgaagtccat gcggaaagta ttggcgcacg catcaaaagc gcccgtacaa
      541 agctgggatt aaccgaatct gatcttgccc gcatgattca tacctacagt gaccccataa
      601 acgactggga atgcggcatc tgtgaagttc ctgctgatca tatagtacca ctggccagtg
      661 cgcttaattg tgacctgatg tggttgttaa cgggaaaatc agaagcaaag gagcagcaac
      721 aatgactggc aatatccatg ataagtatga aggcttatgc ctggcaccgg attcctttgc
      781 aaacaatatc cataatttat tatgcgcagt tatcgtatta caaatgtcag acaacgacgc
      841 aataaaaaga acaggtgatg aagttcttga atttgcacgt tgctatgctg aagcagcagc
      901 tgaaaaagaa ctatccagtt aaatagaaca agtcatctcc agatactata ttacggctta
      961 atcgccgggg attatcacac acttaatcca caggaggttt tattatgact tttataaaac
     1021 ataaggcatc acacaaaaca gcctgcctta ttgcacaaca cgggaaaaat cacatgcata
     1081 ttgcctgctt gtttctgcgt aaagcatacg ggagataata atgcatcaga aaacagcaga
     1141 acacgaacaa accagagtat tgctgaccat caaaaacggg aaagtaatat taattcgtca
     1201 tgttcatgac gatgaatttg tagaaatctt tcaacattcc tgtttattgc agaaaaggca
     1261 ggatatgacg ttattgcacc agcagatgaa gatgaggaat aaatttcatg caatacgctg
     1321 aattccaggc tgaagcaaca gccaatggta tacaaacagg cagtatgact attgattatc
     1381 acgacgccat acgccgtctg gatgctggag aattcgatac tcctaatgtg cgaggtttac
     1441 gtatccttca gtgtctcgcg caagctgacg aagcaggatt actgggaaaa cttccggttg
     1501 agatgaaggt tgcccagtgg cgatggttgt atgtgacgac attcatcaac gaagaagaag
     1561 acaagaacgg cacaattgat gtcccgaatg aacacggaac aacagatcgc gccgtaatat
     1621 ataacgggaa gcatggggtt atgacgatat atcccggccc cattcggttt gccttacagc
     1681 agtatattga atggaattta attcaaaaat acggcgaggc agaaggaatg ggtagagcgc
     1741 tgtttcttta tcagaaaatg ctcattacat acccagataa aggtttcatt gtttcagaca
     1801 tggggcgaga agggcttgaa ctccttctgg atgaaatgat taacgacctg aatactcatg
     1861 gtatgccaga aggccagtga cacattaaat attaagaaga atataattct tccgtttttt
     1921 actaaccatt tatatgaaaa acaaccgtga attaagcaga gtaaaactgc ttttaatcct
     1981 tgccacagta ctgacaataa cagaaatcat tattctcttt attgcgctgt ctgtcagtta
     2041 aaaatatcgg gataccacat accaatgaga ctgtatttca caatagtaat tttactggca
     2101 attatcgcat gcatttacgg attactcgtt ccgttcctta tatccatgaa ggatacgata
     2161 gcagttactt ctggctttgc actggcgttt ctgaccccgc cctgcattta tgccatttac
     2221 aagggtcttt ctttcactaa ggataaaaaa tgaaaaaaat tatttttgct ttagccattg
     2281 ttctgccgac cattggcctt gtcggctgcg atcgcgttga acccggtaat gttggcatca
     2341 aagtaaataa actgggcgac gataaaggcg tcggtgaggt ggtcggtgtt ggtcgctact
     2401 ggactggctg gaatactgaa gtttacatct tcccaacctt caaacaaatg aagacctacg
     2461 atgagccgtt cagcttccag atgagtgacg gcacaaccat cggctatcac atcggtgttg
     2521 cctacaaagt tgatccatcc aaagttacca cggtgtttca gacctaccgc aaaggcgtgg
     2581 atgacattac cgccactgac ctgcgccaga agatcgccga cgcactcaat cgactggcca
     2641 gcaaaatgac caccgacaaa tttatcgacg gcggcaagtc tgaactactg gatgccgcac
     2701 ttaaagacat tcaggaagag atgaccccca tcggcgttca ggtaatgagc ctctcttatg
     2761 taggtaaacc ggaatacccg ccaaccgtta tcgacagcat taacgccaaa gtcacggcaa
     2821 accagaaaac cctgcagcgc gaacaggaag tcaagcaacg tgaagcggag gccaacatgc
     2881 tgcgtgcaga agctgccgga caggctgatg ccattcgaac aaaagccctg gcagaagccg
     2941 atgccattcg tttacgcggc gaagctctgc gccagaaccc tggcgtcatg gagctggaag
     3001 ccatcaacaa gtggaacggt acactgccgc aatacatgac cagtggtgcc aatacaccat
     3061 ttatccagat taaataactt acacgcccgg caaaccgccg ggttaatgga aaatcagatg
     3121 aacaaccaga atactcaacc gcaaataatg aactatgacc cgaatctgac gtcgtgcgga
     3181 cgcatggcaa aacaaaccgt tcgattaact ttcggactat gggaataccg cgaaacattc
     3241 gaagttactg tcggcggcaa tctgaccgga ctggatgtta tcaattgcgc tattgaaagc
     3301 ctgtacgcaa cgctgcctta tgaagaagtc ctggatgagc gcacagggaa aacggatatc
     3361 atggccacca ttaatattgg cgaactgata tgccaggatg aagacctgtc cggggaactc
     3421 tggcttgccg ggatgcttat ctcagcagaa attatcagca ttgaacccgc tacaaacata
     3481 cggctctgaa gttctcacta ttcagagagc aggagaaaaa atgttcgctc tgattaatca
     3541 gggacaactg tataccgaca gtgccggtta cccggtaaaa attgttcgct gcataaacaa
     3601 caccgtgttg tacagaagat gtgaagcgaa tgtttgaagt gtggtgccac tgccgtggat
     3661 gaaagtttta tgaggttggc atgcagacaa tcatctatca gataaccccc agcaaatggt
     3721 gtacggagag agtcctcatt gcatcaacag ggctaaagcc tggcaccatc gagcgagcca
     3781 gaagaaagtc atggatgcag ggaaaagaat accgccatta cgctgtagaa ggtgatccgg
     3841 ggcattacag tgaatgcctg tacaacatcg aagaaattat gcgatggatc gaaaaccaga
     3901 aacaaccagg tgccaaaaat gcaagttccg gttaacctgt taatgctcct ggacgtctgg
     3961 gaggttttat gagtaacgca tcatacccga caggcgttga aaaccatgga ggatcactcc
     4021 gtatatggtt tcactataat ggcaaacgtg tcagagaaaa cctcggtgtt cctgacaccg
     4081 ccaaaaaccg gaagatcgct ggtgagcttc gcacttctgt ttgttttgca atcagaatgg
     4141 ggagtttcga ctacgccgcg cagttcccta attcccctaa cctgaaacac tttggtctgg
     4201 gaaaaagaga gataaccgtt aaggcacttt cggaaaaatg gttggacctt aagaaaattg
     4261 agataggaag taatgcattc agtcggtatc aatccgtggt gagaaacatg cttcctcgca
     4321 taggggaaaa acgtcttgct tcgtcggtaa caaaggaaga tttactgttt atcaggaaag
     4381 atttattgac cgggtatcat aatctttcta acggaaaaac aacgccgatt aaagggaggt
     4441 cagtagttac ggttaattac tacatgacga caattgcagg aatgtttcaa tttgcggctg
     4501 ataacggcta tatcgtgtca aacccattta acggcctgac accattaaag agatccagaa
     4561 cagaaccaga tccgctcaca cgtgacgaat ttattcgttt tattgatgcc tgtcaccatc
     4621 aacaaacaaa aaacctgtgg tccttagcag tatacacagg cattcgtcac ggtgagctaa
     4681 tatctctcgc ctgggaggat attgatttaa aagctaaaac aatgactatc cgtcgtaatt
     4741 atacaaaact cggggagttc actctaccaa aaacagaagc gggaactgat cgtgttattc
     4801 atcttgttca accagctgtt gatgccctga aaagccaggc tgaaatgaca agacttggcc
     4861 ctcagtatca aattgacgtc aagcttcggg agttcggtcg caccgcacgc catgaatgca
     4921 cgtttgtttt taatccgcaa ctggtaaaaa aatgccagca agtcggtcac cactataaag
     4981 cagattccat cagagattcc tgggcatctg cattaaggcg agcaggactg cggcacagaa
     5041 aagcctatca gtctaggcat acttatgcct gctgggcatt atcggcagga gcgaatccaa
     5101 gcttcatagc aaaccaaatg ggccatgcaa atgcacaaat ggtattcaac gtttacggag
     5161 catggatgaa agataacaat atcgggcaaa tagaactact caataagcag ttgacggaga
     5221 gtgtcccata catgccccat agagccagac tctgaaataa tatctttaaa tatcagttgt
     5281 tttagtggta gttgccgtca cattaactgc gtggagggca acaccacgct ttacgccctg
     5341 ccgaaacccg aggttgtcct gcgctggcgt gaacagacca cagatgactt ccgcttctgt
     5401 tttaagtttc cggcgaccat ttcgcatcag gcagcattac gacattgcga tgatttagtg
     5461 actgaatttt tgacccgcat gtcaccgttg gctccgcgca ttgggcaata ctggctgcaa
     5521 ctgcctgcca cattcggccc acgggagctg cctgcgcttt ggcattttct cgattctctc
     5581 cctggcgaat ttaattatgg tgtggaagtc cgccatccac agtttttcgc caaaggggaa
     5641 gaggaacaaa cgcttaatcg cggtttacat cagcgcggcg ttaatcgggt gattttagac
     5701 agccgcccgg ttcatgcagc atgtccacac agtgaagcta ttcgcgacgc tcaacgaaaa
     5761 aaacctaaag ttccggtaca tgctgtactg acggcgacaa atccgctgat ccgttttatc
     5821 ggtagtgatg atatgacgca aaaccgggaa ttatttcagg tctggttaca gaaattagcg
     5881 cagtggcatc agaccactac gccttatctt tttttacata cgccagacat tgcccaggcc
     5941 ccggaactgg tacataccct gtgggaagac ttacgtaaaa cgcttccaga gatcggagca
     6001 gttccggcta ttccacagca atcttctctt ttctgaattt gccacctatc atagacaggt
     6061 gccatcggcc attttaaagg gagtttgtat ggtaagcgcg ctatatgccg ttttaagtgc
     6121 gttgttatta atgaagttct cttttgatgt cgttcgcttg cgtttgcagt accgcgttgc
     6181 ctatggtgac ggtggtttta gcgaactgca aagcgctatt cgcattcatg gtaacgcggt
     6241 ggaatatatt cccatcgcga ttgtgctgat gctgtttatg gaaatgaatg gcgcagaaac
     6301 ctggatggtg catatttgcg gcatcgtttt gcttgctggt cgtctgatgc attattacgg
     6361 ttttcatcac cgtctgtttc gctggcgacg ttccggcatg agcgccacct ggtgtgcgct
     6421 gttgctgatg gtgctggcga atctttggta tatgccctgg gagttggttt tctccctgcg
     6481 ttagcgcaca atacgccact ttctttttcc cggattttta cgttatgtct caccgcgaca
     6541 cgctattttc tgcccctatc gccagactgg gcgactggac ctttgatgaa cgggtagctg
     6601 aagtcttccc ggatatgatc cagcgttccg ttcccggcta ttccaatatt atttccatga
     6661 ttggtatgtt agccgagcgc ttcgttcaac ctggtacgca ggtttacgat ctgggttgtt
     6721 ctctgggcgc ggcgacgctc tcggtgcgtc gcaacattca tcatgataat tgcaaaatta
     6781 ttgccatcga caactccccg gcgatgattg aacgctgccg tcgtcatatt gacgcctata
     6841 aagcccctac gccagtagac gtcattgaag gtgatattcg cgatatcgcc attgaaaacg
     6901 catcgatggt ggtgctgaat tttaccctgc aattcctgga accttccgag cgccaggcat
     6961 tactggataa aatttatcaa ggactgaacc cgggcggcgc gctggtgctt tcggaaaaat
     7021 tcagtttcga agatgccaaa gttggtgaac tgttgttcaa catgcaccac gactttaaac
     7081 gtgccaacgg ttacagcgaa ctggagatca gccagaagcg cagcatgctg gaaaacgtga
     7141 tgctgaccga ttccgtggaa acccataaag cacgcctgca taaagccggt tttgagcata
     7201 gcgagctgtg gttccagtgc tttaactttg gttcactggt ggcattaaaa gcagaggacg
     7261 ctgcatgatc gactttggta acttttattc tctgattgcc aaaaatcatc tttcacactg
     7321 gctcgaaacg ctgcccgcgc agattgctaa ctggcagcgc gagcagcagc acgggctgtt
     7381 taagcagtgg tccaacgcgg tggaatttct gcctgaaatt aaaccgtatc gtctggattt
     7441 attgcatagc gtaaccgccg aaagcgaaga gccactgagc accgggcaaa ttaaacgcat
     7501 tgaaacgctt atgcggaacc tgatgccatg gcgcaaaggg ccgttctcac tgtatggcgt
     7561 caacatcgat accgaatggc gttccgactg gaaatgggat cgtgttctgc cccatctttc
     7621 tgatttaacc ggacgcacca ttctggatgt cggctgcggc agcggttatc acatgtggcg
     7681 catgattggc gcaggcgcgc acctcgcggt ggggatcgac cctacgcaac tgtttctgtg
     7741 ccagtttgaa gcggtgcgta aactactggg taacgatcag cgcgcacatt tgttaccgtt
     7801 aggtattgaa cagcttccgg cactgaaagc ctttgatacc gtcttttcga tgggcgtgct
     7861 ttaccatcgc cgttcgccgc tggagcatct ctggcagttg aaagatcaac tggtgaatga
     7921 gggtgaactg gtgctggaaa cgctggttat tgatggcgat gagaacacgg tgctggtacc
     7981 aggcgatcgt tacgcgcaaa tgcgtaatgt ctatttcatc ccttccgcgc tggcgctgaa
     8041 aaactggctg aagaagtgtg gttttgtaga tatccgcgtt gtggatgtgt gcgttaccac
     8101 tactgaagag cagcgacgca ctgaatggat ggttaccgaa tctctgtccg atttcctcga
     8161 cccacatgat ccgagtaaga cagttgaagg ttatcctgcg cctaaacgcg cggtgctgat
     8221 tgcgcgcaag ccgtaaaggt ctggcattac tgccggatgc ggcttgaatg ccttatccgg
     8281 cctacaaacc cttgctaatt caatctattg cagggactat gtaggcctga taagcatagc
     8341 gcatcaggca gttttacgtt tgcataacct cagcgcccgt ttccgggcgc tattcacttc
     8401 ttacgcctga acagcaggct tatcaaatgc cgttaacttc ggtgcgttac ccgtatattt
     8461 ttcgatatac accagcgcag agttaccggc acaggcattt gccagctgcg agctggggat
     8521 atccgccgtt aacacgttcg cactgccgtt tttacacaag ccgttttcca gatctggcca
     8581 tgcaccttca tgcacgcata ccacgccttt tttgatcccg tcagtcacca ccgcgcctgt
     8641 caaaatctga ccacgtttgt tccagacacg caccagatcg ccattcgcaa taccaaatcg
     8701 agcagcatct tcggtgtgaa tagtgatcgg ttcacgatcg gcaaccgcat attttttacg
     8761 cagttccgca tagttaagct gactatgtaa acggtgtgcc ggatgtgcgg tcagaagctg
     8821 caactgcttc tcgtcggcgg tacccttcca ctcatcaggc gcaagccagg ttggatgtgc
     8881 cgggcaatcc ttatagccaa atttttccag cgttttggag taaatctcaa ttttgccgct
     8941 tggcgtaccc agcgcatttt tcaccggatc ggcgcggaaa tcagcataac gaacgtactg
     9001 ttcgttcttc tcgctgcggc gcatttcgat cagtttattt tgctgccaga aggcattaaa
     9061 catcggcata gtcacgcgtt gcgcacgagc acctttctga gcggcatcat agaaaaattt
     9121 cagccacgcc atttcatctt taccttcggt atagatctct tttccgccag gtttcagtaa
     9181 ttcagcaaga tcggcaaata cgtcaaaatc gttacgcgct tcaaattgcg gagcaacagc
     9241 ctgcttcatc ggcacaatat gctggttgct gtaatcaccg gtcatcgtca agtcattgcg
     9301 ctcaaacgat gtggtgatcg gtaatacgat atctgcatgt ttagcagctg cggtccagta
     9361 gcattcagaa acgacgatca tctccggttt ctgccatgct ttgatcagac gattagtatc
     9421 ctggtgatgg gtaaagttac cgccacccgc ccaccagatc attttgatat tcggatacgt
     9481 ttgttcttta ccgttatgct gatatttgcc accaggattt tccagcgcat cgacgatacg
     9541 ggcaacacga atcgccgtca ttccgccatc atcagcggcc tcgctggcct gtccggcaat
     9601 cgccgcagac atttccggca gcacgccgcc aacacgcgtc ggattgccac cgttggagta
     9661 atgataagag aaaccaaaac cgccgcccgg tgtaccaatc tgccctaaca tcgccgccag
     9721 cgtcaccagc atccagtgtt tctgttcacc atattgctgg cgctgaattc cccagccagc
     9781 catcagcatt gtgcgattcg ccgccatcag ttcagcgagt ttgactatct gcgcttccgg
     9841 tacgccagta atttccgccg cccaggccgc gctcttcgga gtattatcgc tcttacctgt
     9901 cagatactct tcaaactgcg gatacccggt ggtgtatttt tcaaggaaca ctttatcgtg
     9961 tttaccttgt gtcatcaggg tatgcgcaat ccctaacatc agtgccacgt cggtgcccat
    10021 attcggcgcg atccaggtgg cattatcacc aaagaactcg atggtttcgg agcggatagg
    10081 atcaatggca atcactggtt tgccagattt tttcagctga tggaagtatt ccagcccttg
    10141 ctcatcggta ctgctccagg caatttttaa ggtattcagc gggttcatcc cccacagcac
    10201 cacagcctgg ctgttttcca gaatcagcgg ccaggaggtc tgctgttcat acacctctac
    10261 agaaccgacc acatgcggca tgatcacctg tgctgccccg gttgaataat cgccgctatg
    10321 cccggaataa ccgcccgcca ggttcatata acgttgcagt aaggtttgcg ctttatgcaa
    10381 cacgccagaa gagcgccagc cgtatgatcc ggcaaaaata gcagatggtc cgttagcttt
    10441 gcgaatgcga tcgtgttgct catgaatcag ttttaatgct tgctcccagc tcacctgtac
    10501 ataagtatct tcgccacgac ctttcgccgg ttgcagtgga ttatcgagat agctttttct
    10561 taccatcgga tgctgaatac gtgccgtggt gtgtacctga tccgccgccg tagactgtaa
    10621 ggagttcggt atggttttcg ccagcgcgcc tgttgaagaa acaatcttgc cgtccttcac
    10681 ttctacgttc atcgctcccc aacgtcccgc ggtgaggatt ttaccgccct tctcttctgc
    10741 ccatgcgggg agcggtgctg ccgatgtcac caccagcgct ccagcggcaa taccgctgtg
    10801 tttaataaat tcacgtcttg ttaatgtcat aacttcctcc ctgatcaacg aggatcactg
    10861 tttctcggta atatctttgg cgttgtactg gaaataccgc gttaaaatgt ccagttcgtt
    10921 ttcgctcatg ctggttcgcg cacccattcc tttggcaatg gacggccatg cattgacggt
    10981 gtaatggtca gcggcaatag gcgcatggca accagcgcaa taggtatcgt caagtttttc
    11041 agcgtattgc catagcggtt tacggtccgc taatgcggga tcggtaagcg caccctgtaa
    11101 agacgcctga cgccattgat tgccgtattc gtcagcctgc cattccccgt ttaccgtgag
    11161 cgccttgata ccttcttcac ttaatgtggc tagcgccagc cgttgacctg ccgccaggta
    11221 gagcgtgttt tcactgccct gcatttgata accctgcaac agaacgatcg gctgtttgcc
    11281 actggcatca acgacggtga gatcggtacc aggattcacg gtagccagct cgcctatgcg
    11341 agaagttttg aaaggataaa tatgtgcgcc attagtcact gaagtagcgg cctgactttc
    11401 cagctcatgc gccgcgttgt catccatttt tatttctggc ggaaaatggg caatgccttt
    11461 atgacaatcg atacaggttt cgccgccctt ttgtgctttg ttatgcattt tctgcgcaga
    11521 ttcactttgc gaggcaatat ccatggcatc aaaagaatgg caactacggc acgttgcaga
    11581 gtcagttgct tttaattctt tccatactgt ttcggccatt tcctggcgat gagtttcgaa
    11641 cttatcgtca ctgtctattt tgccgctaac aaattcatga taaatatctt tagatgcttt
    11701 taatttagca aataaataat ccatccctga ctttggaata tggcaatctg cacattccgc
    11761 acgtatccct ttctggttcg aaaagtggac agttccctga tattcctcaa aaggtttact
    11821 catcgagtgg caggaaaggc aaaatgctgt atccgacgtt ttatgtaagg ctttttgcgc
    11881 cagcaataac ccgccgccac caaccacaac cgctatcagc aaaaacaa
//
LOCUS       DAZGLJ010000138        11923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000138 DAZGLJ010000000
VERSION     DAZGLJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.138"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(97..861)
                     /gene="nfeF"
                     /locus_tag="ACHK49_003312"
                     /gene_synonym="yqjH"
     CDS             complement(97..861)
                     /gene="nfeF"
                     /locus_tag="ACHK49_003312"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO9263915.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPLSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1138..1761
                     /gene="nfeR"
                     /locus_tag="ACHK49_003313"
                     /gene_synonym="yqjI"
     CDS             1138..1761
                     /gene="nfeR"
                     /locus_tag="ACHK49_003313"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO9263916.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(1915..3435)
                     /gene="aer"
                     /locus_tag="ACHK49_003314"
     CDS             complement(1915..3435)
                     /gene="aer"
                     /locus_tag="ACHK49_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO9263917.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            3853..5232
                     /gene="ygjG"
                     /locus_tag="ACHK49_003315"
     CDS             3853..5232
                     /gene="ygjG"
                     /locus_tag="ACHK49_003315"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO9263918.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5274..5606)
                     /locus_tag="ACHK49_003316"
     CDS             complement(5274..5606)
                     /locus_tag="ACHK49_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO9263919.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            5825..6808
                     /gene="ebgR"
                     /locus_tag="ACHK49_003317"
     CDS             5825..6808
                     /gene="ebgR"
                     /locus_tag="ACHK49_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO9263920.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            6992..10084
                     /gene="ebgA"
                     /locus_tag="ACHK49_003318"
     CDS             6992..10084
                     /gene="ebgA"
                     /locus_tag="ACHK49_003318"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO9263921.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQENEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            10081..10530
                     /locus_tag="ACHK49_003319"
     CDS             10081..10530
                     /locus_tag="ACHK49_003319"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO9263922.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            10593..>11923
                     /locus_tag="ACHK49_003320"
     CDS             10593..>11923
                     /locus_tag="ACHK49_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9263923.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTVGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIV"
BASE COUNT         2841 a         3216 c         3025 g         2841 t
ORIGIN      
        1 cataatcgct tggtcgctgg ttcaagtcca gcaggggcca ccagatatag caaaggctga
       61 cgagaaatcg tcagcctttt tctttttata tatcagttac tttgcgtgcc agtaagccgc
      121 tgcacgtacc cgctgtgggt catactgttc cgcttcaaag cggcggctta aattcttaac
      181 gactttacct tcgccggtta tccagatgaa gtaatcatcg gcagggattt gcatctgcgc
      241 cagacgcgca tctaccgcct gctcatcatg tgccagccat tcgatattaa aaccatcaag
      301 gtgcgcgaga taatcctgac aggcgttatc ccgcacgcta accagcgcac taacttgcgg
      361 tttaacggca agtttgctca acgtttccag gcggcggcgc agtgcgggca ttccggattc
      421 atcgcagaca tacagctgat acgcgtaatc ttccggcacc accagcgaac cgcgcggacc
      481 tgccaccgta agtttatcgc ccggttgcgc ctgcatcgcc cagccgctgg cgaccccacc
      541 gtcgtgaata aagaaatcga tcgccagttc atggcgtagt tcgtcataca gcggcgtata
      601 gtcacgcgac agtgggcgtg gtccttccgg ccagacgatg ccctcttccg ttaccgttgg
      661 cggcacaaag tgagcgtcag gttgaggaaa gaagagtttg ctgtgatcgt caaagccgcg
      721 cgatgtaaaa ccgtccagcg cctcgccgcc gaggacaatg cgctgaaaac cggcgctgat
      781 gcgctcaacg cgtaacacag tcagttcacg gaagcgcaga tcattgcgaa cgcgctgcgg
      841 gtagcggggg gagttattca ttgttatcgc cttcgtgata gtaatcagat atatctaaat
      901 gattttcgta aatgataatg attgttaatc atgataaatg caagcgattt gtagaactga
      961 tatatctgaa gtatgtgaag gcattcagtg ccatagcaat gctgccggat gcggcgtgaa
     1021 cgtcttatcc ggcctggatg tgaggtgctt ccacgtttaa aaatcatttt tgtacttgca
     1081 gtaatgctca catcagatat aaattagata tatctaatca agcaaaagga ggctgatatg
     1141 agccatcatc acgaagggtg ttgtaaacat gaaggccagc cacgccatga gggctgctgc
     1201 aaaggtgaga agtcagaaca cgagcactgc ggacacggtc accagcatga acacggtcaa
     1261 tgctgcggtg gtcgccacgg tcgcggcggc ggtcgtcggc aacgtttctt tggtcacggt
     1321 gaattgcgtc tggtgattct ggatattctc tcgcgcgatg acagccacgg ttacgaattg
     1381 attaaagcga ttgagaacct aacccagggg aattacaccc caagcccggg cgtcatctac
     1441 ccaacgctgg attttctgca ggagcagtcg ctgattacca tccgcgaaga ggaaggtggt
     1501 aagaagcaga ttgcgctgac cgaacaaggc gcgcagtggc tggaagaaaa ccgcgaacag
     1561 gtggagatga ttgaagaacg catcaaagcg cgttgcgttg gcgcggcgct gcgccagaac
     1621 ccgcaaatga agcgggcgct ggataatttt aaagcggtgc tggatttaca cgtcaaccag
     1681 agcgatatca gtgatgcaca aataaaaaag atcattgcgg tgatcgaccg cgccgctttt
     1741 gatattacgc aactggatta atcgccgcat ccgccagtgg cgcggtgcaa ttgccggatg
     1801 cgacgcttga cgcgccttat ccggcctaca cccgctacac accccgcagg cctgataaga
     1861 tgcgccagca tcgcatcagg cattgtgctc caaccgccgg atccggcata ccgattaatg
     1921 cagcaccgtc accgcgtctt ccagtcggct ggcgcggtgt ttcaccatcg ccgacacctg
     1981 cgcactctct tccaccagct cggcattttt ctgggtgatc aggttaagct catccactgc
     2041 acgggtcagg ctggaaagcc catcggcctg ttccagcgtt gaatggctaa tctgggcgat
     2101 caactgggtg acgtttttca cctgtgccac aatatcttcc atcgtccgtc cggcggcgtg
     2161 tacctgctgc gaaccggatt gcaccttatc agcactggca tcaatcagct tgcgaatatc
     2221 gttggcagca ttagcgctgc ggctggctaa atgacgcact tcccctgcca ccactgcaaa
     2281 acctttgccc tgttcgccgg cacgcgccgc ttccaccgcc gcattcaggg ccagaatatt
     2341 ggtctgaaac gcaatatcgt taatcagcga agtaatggtg ccaatgcgct gggtactgtc
     2401 ggcgatatcg tccattgtct tgatcaccgt ggtcatcgcc tccccaccct gcactgccgc
     2461 attactggcg gtgatagaaa gtttatcggc agccgacgcc gtggcagagt tctgtttcac
     2521 cgacgccgcc atttggttca tggtcgccac cgtttgctga acgttatcaa ctgtctgctg
     2581 ggtatgttcg ttcagttcat cagtgccttt cgccagcgtc tcactgccat ttctgacact
     2641 ggacacctgg cttgagacat cgttaattag ccaacggcac atcaggccaa gctgccctac
     2701 cgcgcgtaat gtcagcccca gctcatcgct gcgattcagg tgctcaacac tattacgctc
     2761 tccggtcgcc accttcagcg cctgacgggc gacattttct atcgggcgca caatctgcca
     2821 ttcaaaacaa gcgcttgcca acaataccac taacacacag aggaaatacg tcaccaccgg
     2881 ggcagcaaca aaccaaagca tggccgccag caagataaac atcagggtca tcactccacg
     2941 cgcccgccag cgaagcggta atgaaggcag tttacccagc cagcctttac gcaccaccag
     3001 gcctttatga atacgcttac tggtacgtcc ggcgttcagc gctttgtaca gcggctccac
     3061 cgccgcaatc tcttcatccg tcgcccgggt acgaatcgac atatagccac tgatttttcc
     3121 ctcgcgcacc atcggtaccg cattggcccg cacccaataa tggtcgccat ttttgcggcg
     3181 atttttcacg atgccgctcc agggctcccc ttttttcagg gtgaaccaca tatccgcaaa
     3241 cgccgctttt ggcatatccg ggtgacgcac catgttgtgc ggctgccctt gtaactcttg
     3301 caaggtaaag ccgctcacct gcacaaaagt gtcattagca tgagtgatat agctttgcag
     3361 atcggtagtg gacatcagag tggtatcgtc cgccagcggg gtattttgct gggtgacata
     3421 cggatgagaa gacatgttcg cgtcctgtgc aggttatatg gttgttaact tcttgtcaga
     3481 gtttatgtcg gccccgctgc ggttatcttt aaccgattaa tttgatttag atcgcaattt
     3541 gcgattaaag cacaaatcta attctttgat ttaaaatact ttcactctgt tactatacga
     3601 aaacgttaat tatcttgccc aaaaatcagg caattattgc cctgaaaacg tgcatttgcg
     3661 cagcaatcat caaatccata cccgacaaaa accgtgcaaa ataacaacaa atgttaacag
     3721 atagcattaa atattgcaca aatgataacc gaatttgtgt ttatcccgat tttcgcgatc
     3781 gcagccggag tggcgcaatc cctgcaatac ttaaatcggt atcatgtgat acgcgagcct
     3841 ccggagcata ttttgaacag gttaccttcg agcgcatcgg ctttagcgtg cagcgcccac
     3901 gccctgaatc tcattgagaa gcgaacgctg gatcatgagg agatgaaagc acttaaccga
     3961 gaggtgattg aatacttcaa agagcatgtc aatccggggt ttttagagta tcgcaaatct
     4021 gttaccgccg gcggggatta cggagccgta gagtggcaag cgggaggttt aaatacgctt
     4081 gtcgacaccc agggacagga gtttatcgac tgcctgggag gttttggaat tttcaacgtg
     4141 gggcaccgta atccagttgt ggtttccgcc gtacagaatc aacttgcgaa acaaccgctg
     4201 cacagccagg agctgctcga tccgttacgg gcgatgttgg cgaaaaccct tgctgcgcta
     4261 acgcccggta aactgaaata cagcttcttc tgtaatagcg gcaccgagtc cgttgaagca
     4321 gcgctgaagc tggcgaaagc ttaccagtca ccgcgcggca agtttacttt tattgccacc
     4381 agcggcgcgt tccacggtaa atcacttggc gcgctgtcgg ccacggcgaa atcgaccttc
     4441 cgcaaaccgt ttatgccact gctgccgggc ttccgtcatg tgccgtttgg caatatcgaa
     4501 gccatgcgca cggctcttaa cgagtgcaaa aaaaccggtg atgatgtggc tgcggtgatc
     4561 ctcgaaccga ttcagggtga aggtggcgta attctgccgc cgccgggcta tctcaccgcc
     4621 gtacgtaagc tatgcgatga gttcggcgca ctgatgatcc tcgatgaagt acaaacgggc
     4681 atggggcgca cgggcaagat gttcgcctgc gagcatgaga acgtacagcc ggatatcctc
     4741 tgccttgcca aagcgctcgg cggcggcgtg atgccgattg gcgcgaccat cgccactgaa
     4801 gaggtgtttt cagttctgtt cgacaaccca ttcctgcata ccaccacctt tggcggcaac
     4861 ccgctggcct gtgcggcggc gctggcgacc atcaatgtgt tgctggagca gaacttaccg
     4921 gctcaggctg agcaaaaagg cgatatgttg ctggacggtt tccgtcaact ggcgcgggaa
     4981 tatcccgatc tggtacagga agcgcgtggt aaagggatgt tgatggcaat tgagtttgtt
     5041 gataacgaaa tcggctataa ctttgccagc gagatgttcc gccagcgcgt actggtggcc
     5101 ggaacgctca ataacgccaa aacgatccgc attgaaccgc cactgacact gaccattgaa
     5161 cagtgtgaac tggtgatcaa agcggcgcgt aaggcgctgg cggccatgcg agtaagtgtc
     5221 gaagaagcgt aatatcaaat cggatggcga tacgacgtcg ccatccgatc tttttatacg
     5281 acgcgtacgc ccgcaggcat catccgctcc ggcgttaaca acacgctttc actgccgtca
     5341 tcggtttccg cacacaacag catgcattcc gacgtttcac cacgcatttt cgctttttgc
     5401 agattgcata agaccaccac cgttttcccc atcagctctt cttcgctgta gtacggcacc
     5461 aggctggtca cggtttgcag tgttttttcc cccacatcaa cctgtacgat gtacagcttg
     5521 tcggcgtttt catggcgttt cacttccaca atctttccga cgcgcatttc cagacgtgca
     5581 aaatcagcgt aagccacggt ttccattgtt tcctcccttc gggtaaaatt tcactaaact
     5641 atagcaaaag tttttctcaa tcctgtaggc taaaaatgga gaatgcaggc gtgatcacat
     5701 tcgtgagccg ctgtgttacc gttacagcgt caaagaaacg cgctttattt actgaaaaca
     5761 ggtgacccga taagcacttc ctctacaatg ggggcgcaca tcagggaaag taaaaaaggt
     5821 aaacatggca acactaaaag acatcgcaat cgaagctggc gtatccctgg cgacagtatc
     5881 cagggtctta aatgacgatc cgacattgaa tgtgaaagaa gagacgaaac atcgcattct
     5941 cgagatcgcc gaaaagctgg agtacaagac cagtagtgcc cgtaaactcc agacaggtgc
     6001 agtcaaccaa caccatattc tggctatcta cagctaccag caggagctgg agatcaacga
     6061 tccttactat ctggcgatcc gccacggcat tgaaacccag tgcgaaaagc tgggcatcga
     6121 gctcaccaac tgttatgaac acagcggctt accagacatt aaaaacgtca ccggtatttt
     6181 aattgtcggc aaacccacgc ccgccctgcg cgccgctgcc agcgcgttga ccgacaatat
     6241 ctgttttatc gactttcacg aacccggcag cggttacgat gcggtggata tcgatctggc
     6301 acgcatcagt aaagaaatca tcgacttcta tatcaaccag ggcgttaatc gtattggttt
     6361 tattggcggt gaagatgagc ctggcaaggc ggatattcgt gaggtcgcct ttgcggaata
     6421 tggccgactg aaacaagtgg tacgcgaaga ggatatctgg cgcggcggtt tttccagttc
     6481 gtcgggttat gaactggcaa aacaaatgct ggcgcgggaa gactatccga aggcactgtt
     6541 tgttgcttcc gattccattg ctatcggcgt actgcgggca attcatgaac gaggcctgaa
     6601 catcccacag gatatttcgc ttatcagcgt taacgatatc cccaccgcgc gatttacctt
     6661 tccgccgctc tccaccgtgc gcatccattc cgagatgatg ggaagtcagg gcgttaacct
     6721 ggtgtatgaa aaagcccgcg atggtcgcgc gctgccgctg ttagtcttcg ttcccagcaa
     6781 attaaaactg cgcggcacga cccgttaaat ccccttacac actgtccggc aatcgttttt
     6841 gccggacagt gctgccgttt attttcgtga tccagttaaa gtaaatgcat ttacctgcta
     6901 ctttttagta aaaattttac taaactcccc agcaattaca caaactacca tcaccatgaa
     6961 tggttccgat ttctctctac cgggaggccc tatgaatcgc tgggaaaaca ttcagctcac
     7021 ccacgaaaac cgacttgcgc cgcgtgcgta ctttttttca tatgattctg ttgcgcaagc
     7081 gcgtaccttt gcccgcgaaa ccagcagcct gtttctgccc ttaagcggtc agtggaattt
     7141 ccactttttt gaccatccgc tgcaagtacc agaagccttc acctctgagt taatggctga
     7201 ctgggggcat attaccgtcc ccgccatgtg gcaaatggaa ggtcacggca aactgcaata
     7261 taccgacgaa ggttttccgt tccccatcga tgtgccgttt gtccccagcg ataacccaac
     7321 cggtgcctat caacgtattt tcaccctcag cgacggctgg cagggtaaac agacgctgat
     7381 taaatttgac ggcgtcgaaa cctattttga agtctatgtt aacggtcagt atgtgggttt
     7441 cagcaagggc agtcgcctga ccgcagagtt tgacatcagc gcgatggtta aaaccggcga
     7501 caacctgttg tgtgtgcgcg tgatgcagtg ggcggactct acctacgtgg aagaccagga
     7561 tatgtggtgg tcagcgggga tcttccgcga tgtttatctg gtcggaaaac acctaacgca
     7621 tattaacgat ttcactgtgc gtaccgactt tgacgaagcc tattgcgatg ccacgctttc
     7681 ctgcgaagtg gtgctggaaa atctcgccgc ctcccctgtc gtcacgacgc tggaatatac
     7741 cttgtttgat ggcgaacgcg tggtgcacag cagcgccatt gatcatttgg caattgaaaa
     7801 actgaccagc gccagctttg cttttactgt cgaacagccg cagcaatggt cagcagaatc
     7861 cccttatctt taccatctgg tcatgacgct gaaagacgcc aacggcaacg ttctggaagt
     7921 ggtgccacaa cgcgttggct tccgtgatat caaagtgcgc gacggtctgt tctggatcaa
     7981 taaccgttat gtgatgctgc acggcgtcaa ccgtcacgac aacgatcatc gcaaaggccg
     8041 cgccgttgga atggatcgcg tcgagaaaga tctccagttg atgaagcagc acaatatcaa
     8101 ctccgtgcgt accgctcact acccgaacga tccgcgtttt tacgaactgt gtgatatcta
     8161 cggcttgttt gtgatggcgg aaaccgacgt cgaatcgcac ggctttgcta atgtcggcga
     8221 tatcagccgt attaccgacg atccgcagtg ggaaaaagtc tacgtcgagc gcattgttcg
     8281 ccatattcac gcgcagaaaa accatccgtc gatcatcatc tggtcgctgg gcaatgaatc
     8341 cggctatggc tgtaacatcc gcgcgatgta ccacgcagcg aaggcgctgg atgacacgcg
     8401 actggtgcat tacgaagaag atcgcgatgc tgaagtggtc gatattattt ccaccatgta
     8461 cacccgcgtg ccgctgatga atgagtttgg tgaatacccg catccgaagc cgcgcatcat
     8521 ctgtgaatat gctcatgcga tggggaacgg accaggcggg ctgacggagt accagaacgt
     8581 cttctataag cacgattgta ttcagggaca ttatgtttgg gagtggtgcg accacgggat
     8641 ccaggcgcag gatgacaacg gcaacgtctg gtataaattc ggcggcgact acggcgacta
     8701 tcccaacaac tataacttct gtcttgatgg tttgatctat tccgatcaga cgccgggacc
     8761 gggcctgaaa gagtacaaac aggttatcgc gccggtaaaa atccacgcgc tggatctgac
     8821 tcgcggcgag ctgaaagtcg aaaataaact gtggtttacc acgcttgatg actacaccct
     8881 gcacgcagag gtgcgcgtcg aaggtgaaac gctcgcgacg cagcagatta aactgcgcga
     8941 cgttgcgccg aacagcgaag cccccttgca gatcacgctg ccgcagctgg acgcccgcga
     9001 agcgttcctc aacattacgg tgaccaaaga ttcccgcacc cgctacagcg aagccgggca
     9061 ttctatcgcc acttatcagt tcccgctgaa ggaaaacacc gcgcaaccag tgcctttcgc
     9121 accaaataat gcgcgtccgc tgacgctgga agacgatcgt ttgagctgca ccgttcgcgg
     9181 ctacaacttc gcgatcacct tctcaaaaat gagtggcaaa ccgacatcct ggcaggtgaa
     9241 tggcgagtcg ctgctgaccc gcgagccaaa gatcaacttc ttcaagccaa tgatcgacaa
     9301 ccacaagcag gagaacgaag ggctgtggca accgaatcat ttgcagatca tgcaggaaca
     9361 tctgcgcgac tttgccgtag aacagagcga tggtgaagtg ttgatcatca gccgcacagt
     9421 tattgccccg ccggtgtttg acttcgggat gcgctgcacc tacatctggc gcatcgctgc
     9481 cgatggccag gttaacgtgg cgctttccgg cgagcgttac ggcgactatc cgcacatcat
     9541 tccgtgcatc ggtttcacca tggggattaa cggcgaatac gatcaggtgg cgtattacgg
     9601 tcgtggaccg ggcgaaaact acgccgacag ccagcaggct aacatcatcg atatctggcg
     9661 cagcaccgtc gatgccatgt tcgagaacta tcccttcccg cagaacaacg gcaaccgtca
     9721 gcatgtccgc tggacggcac tgactaaccg ccacggcaac ggtctgctgg tggttccgca
     9781 gcgcccaatt aacttcagcg cctggcacta tacccaggaa aacatccacg ctgcccagca
     9841 ctgtaacgag ctgcagcgca gtgatgacat caccctgaac ctcgaccacc agctacttgg
     9901 cctcggctcc aactcctggg gcagcgaggt gctggactcc tggcgcgtct ggttccgtga
     9961 cttcagctac ggctttacgt tgctgccggt ttctggcgga gaagctaccg cgcaaagcct
    10021 ggcgtcgtat gagttcggcg cagggttctt ttccacgaat ttgcacagcg agaataagca
    10081 atgaggatca tcgataactt agaacagttc cgccagattt acgcctctgg caagaagtgg
    10141 caacgctgcg ttgaagcgat tgaaaatatc gacaacattc agcctggcgt cgcccactcc
    10201 atcggtgact cattgactta ccgcgtggag acagactccg cgaccgatgc gctatttacc
    10261 gggcatcgac gctattttga agtgcattac tacctgcaag ggcagcaaaa aattgaatat
    10321 gcaccgaaag agacattaca ggtagtggaa tattatcgtg atgaaactga ccgtgaatat
    10381 ttaaaaggct gcggagaaac cgttgaggtc cacgaagggc aaatcgttat ttgcgatatc
    10441 catgaagcgt atcggtttat ctgcaataac gcggtcaaaa aagtggttct caaagtcacc
    10501 atcgaagatg gttatttcca taacaaataa caactacggc ggcaaaagga gtttgccgcc
    10561 accgctaccc tacacatttt cggagatgtg ttatgtctga taccaaacgt aatacaatcg
    10621 gcaaattcgg cttgctctcg ctgacttttg ccgccgttta cagctttaac aacgttatca
    10681 ataataatat tgagcttgga ctggcctcgg caccgatgtt tttcctcgcg acgatttttt
    10741 attttattcc cttctgtctg atcatcgcag aatttgtttc gttaaataaa aactcagaag
    10801 ccggtgtcta cgcgtgggta aaaagttcgc tgggcggacg ttgggcattt attactgcct
    10861 atacctactg gttcgtaaac ctgttctttt tcacctcact gttgccgcgc gttattgctt
    10921 atgcttcgta tgccttcctc ggatacgaat atattatgac gccggttgcc accaccatta
    10981 tcagtatggt gctgttcgcc ttctccacct gggtttccac caacggggcg aaaatgttgg
    11041 ggccaattac ctccgtcact tcaacgctga tgctgctgtt aacgctctcc tacattttac
    11101 tggcaggtac ggcgctggtt ggcggcgtac agcctgctga ccccatcacc gttgacgcga
    11161 tgatcccgaa cttcaactgg gcgttcctcg gcgttaccac ctggatcttt atggccgcag
    11221 gtggcgcgga gtccgtcgct gtgtacgtta acgacgtcaa aggcggttcg aaatcgttcg
    11281 ttaaagtgat catcctcgcc gggattttta tcggcgtact gtattccgtc tcctcggtgc
    11341 tgattaacgt cttcgtcagc agcaaagagt tgaaatttac cgtcggatcg gtgcaggtat
    11401 tccacggcat ggcggcgtat tttggtctac cggaagcgtt gatgaatcgc tttgtcggtc
    11461 tggtgtcctt taccgcgatg ttcggttccc tgctgatgtg gaccgcaacg ccggtgaaaa
    11521 ttttcttctc cgaaatcccg gaaggcatct ttggtaagaa aaccgtcgaa ctgaacgaaa
    11581 acggcgttcc ggcgcgcgca gcgtggatcc agttcctgat cgtcatcccg ctgatgatta
    11641 tcccgatgct cggttccaat accgtgcagg atctgatgaa tactattatt aatatgaccg
    11701 ccgcagcgtc catgcttccg ccgttattca tcatgctggc ttacctgaat ttacgcgcca
    11761 aattagatca cctgccacgc gatttccgta tgggctcccg ccgcaccggt attatcgttg
    11821 tttcaatgct gattgcgata tttgccgtag ggtttgtcgc ttcgacattc ccgactggcg
    11881 cgaatattct gaccatcatt ttttataacg tcggcggtat tgt
//
LOCUS       DAZGLJ010000139        11850 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000139 DAZGLJ010000000
VERSION     DAZGLJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.139"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..407
                     /locus_tag="ACHK49_003321"
     CDS             <1..407
                     /locus_tag="ACHK49_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO9263924.1"
                     /translation="FPIIKQSKNLNFLNVSGKNNCPTMQLMLFNLFSPALKLNTGLAI
                     LSPGAFEVHSDGIDVDNELFHYPIKKAYTPYNIHTYKTEEVVNQRNIKVKNMTLDEIN
                     NTYCNNDYYNQAIREEPIDLLDRSFSSSSWPF"
     gene            731..2119
                     /gene="narU"
                     /locus_tag="ACHK49_003322"
     CDS             731..2119
                     /gene="narU"
                     /locus_tag="ACHK49_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO9263925.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2201..5941
                     /gene="narZ"
                     /locus_tag="ACHK49_003323"
     CDS             2201..5941
                     /gene="narZ"
                     /locus_tag="ACHK49_003323"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO9263926.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5938..7482
                     /gene="narH"
                     /locus_tag="ACHK49_003324"
     CDS             5938..7482
                     /gene="narH"
                     /locus_tag="ACHK49_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO9263927.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7482..8177
                     /gene="narW"
                     /locus_tag="ACHK49_003325"
     CDS             7482..8177
                     /gene="narW"
                     /locus_tag="ACHK49_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO9263928.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRSDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            8174..8854
                     /gene="narI"
                     /locus_tag="ACHK49_003326"
     CDS             8174..8854
                     /gene="narI"
                     /locus_tag="ACHK49_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO9263929.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKREMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8933..9826
                     /locus_tag="ACHK49_003327"
     CDS             8933..9826
                     /locus_tag="ACHK49_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO9263930.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9922..10767)
                     /gene="nhoA"
                     /locus_tag="ACHK49_003328"
     CDS             complement(9922..10767)
                     /gene="nhoA"
                     /locus_tag="ACHK49_003328"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO9263931.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10940..11509
                     /locus_tag="ACHK49_003329"
     CDS             10940..11509
                     /locus_tag="ACHK49_003329"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO9263932.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11513..11740)
                     /gene="pptA"
                     /locus_tag="ACHK49_003330"
                     /pseudo
     CDS             complement(11513..11740)
                     /gene="pptA"
                     /locus_tag="ACHK49_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
BASE COUNT         2844 a         3045 c         3086 g         2875 t
ORIGIN      
        1 tattccccat aataaaacag agcaaaaact taaatttttt aaatgtttct gggaagaaca
       61 attgccctac tatgcagctc atgttattta atttgttttc cccagcactt aagcttaata
      121 ctggcctggc aattctttcg cctggtgcat ttgaagttca ctctgacgga atagatgtgg
      181 ataacgaatt gtttcactat cctattaaaa aagcatatac cccatataat atacacactt
      241 acaagacaga ggaagttgta aaccagagga atataaaagt taaaaacatg accttagatg
      301 aaataaacaa tacttactgt aataacgatt attacaatca ggcaataaga gaggaaccga
      361 tagaccttct ggacagatcg ttttcctcca gttcatggcc tttttagtga ataaatcatc
      421 actaattcta taagtgtctg gtcttgttga taatatagtt aatatgtaac cctttagatt
      481 cacgatctga agggttgaaa catgacatac gttcaaagat taacccccgt ttgaaggtta
      541 gcttgttagt tacatttagt aacacatatc tggctactta atccccattc tgcccccgcg
      601 gttacgcttt cttatctatt ctttcaatca gcacttcacc caattcatcc ttaattctaa
      661 taactggcta ttgcgcctta cctcctgttt tgtctttttt cattaccaat gtgtgcatgt
      721 gaggaacaat atggcactgc aaaatgagaa aaatagtcgt tatcttttac gcgactggaa
      781 accagaaaat ccggccttct gggaaaataa aggaaaacat attgctcgaa gaaacctctg
      841 gatatcagtc agttgtctac ttcttgcctt ctgtgtctgg atgctattta gcgcagttac
      901 cgttaatctc aataaaatcg gttttaattt tactaccgat caactctttt tattaaccgc
      961 attaccctcc gtttctggcg cattattgcg tgttccctac tcctttatgg tgcctatatt
     1021 cggtggacgc cgatggacgg tttttagtac tgcaatcctg attattcctt gcgtctggct
     1081 cggaattgcc gtgcaaaatc cgaatactcc ttttgggata tttatcgtta tcgctttgct
     1141 atgcggtttt gcaggtgcaa actttgcttc gagcatgggc aatatcagtt tcttctttcc
     1201 aaaagccaaa caagggagcg ctcttgggat taatggcgga ttaggaaact taggtgtaag
     1261 tgtaatgcag ctggttgcac cgctggtcat ttttgtacct gtatttgcct ttctcggcgt
     1321 caatggcgta ccgcaggccg acggttcggt gatgtcgctg gcgaatgccg catggatttg
     1381 ggtaccgcta ctggcgattg ccacgatcgc cgcatggtca gggatgaatg atatcgccag
     1441 ttcacgcgcc tcaattgccg accagctccc tgtcttacaa cgcctgcatc tctggctgct
     1501 gagcctgctt taccttgcca ccttcggttc gtttatcggt ttttctgcgg gttttgccat
     1561 gctggcaaaa acccagttcc cggatgtgaa tattctgcgc ctggcgttct ttggcccatt
     1621 tatcggtgcc atcgcgcggt cggttggtgg tgctatttcc gataagttcg gcggcgtgcg
     1681 ggtgacgttg atcaacttta tttttatggc gattttcagt gccctgctgt tccttacctt
     1741 accgggcaca ggctccggta atttcatcgc cttttacgcc gtatttatgg ggctgtttct
     1801 gactgcgggt ctgggaagtg gttctacttt ccagatgatc gccgtcatct ttcgccagat
     1861 aaccatttat cgggtaaaga tgaaaggcgg tagtgatgag caagctcaga aagaagccgt
     1921 caccgaaacg gcggcggctc tgggctttat ctcagccatt ggcgcagtgg gcggcttttt
     1981 tattccgcag gcgtttggca tgtcgctcaa tatgaccggc tctccggtcg gcgcgatgaa
     2041 agtgttttta atcttctaca tcgtttgtgt gctgctgacc tggctggttt atggtcggcg
     2101 gaagttcagc caaaaataag agatcgatgc ctgatgtaca accgtgtcgc atccgacaag
     2161 gcatcagatc ccaattcatt cctggagcag gagtcatgtc atgagtaaac ttttggatcg
     2221 ctttcgctac ttcaaacaaa agggcgaaac ctttgccgat ggtcacggac aggtgatgca
     2281 tagcaaccgc gactgggagg acagctatcg ccagcgttgg cagttcgaca aaatcgtgcg
     2341 ttccacccac ggtgttaact gtacaggctc ctgtagctgg aaaatctacg ttaaaaatgg
     2401 tctggtgacc tgggaaatcc aacagaccga ctacccgcgc actcgccctg acctgcccaa
     2461 tcatgaacct cgcggctgcc cgcgtggcgc aagttactcc tggtatcttt acagcgctaa
     2521 ccgcctgaaa tacccgctca ttcgtaaacg actgattgaa ctgtggcgcg aagccctcaa
     2581 gcaacacagc gatccggtac tggcgtgggc atcgattatg aacgatccgc aaaagtgcct
     2641 gagctacaaa caagtgcgtg ggcgcggcgg gtttatccgc tccaactggc aggaactaaa
     2701 ccagctgatt gccgccgcta acgtctggac catcaaaacc tacggcccgg atcgcgttgc
     2761 cggtttctcg ccgatcccgg cgatgtcgat ggtttcttac gccgccggaa cgcgttatct
     2821 gtcgctgctt ggcggcacct gtttaagttt ctacgactgg tattgcgacc tgccgcccgc
     2881 ctcgccgatg acctggggcg agcaaaccga cgtaccggaa tctgccgact ggtataactc
     2941 cagctacatc atcgcctggg ggtctaacgt accgcagaca cgtacgccgg acgcccactt
     3001 ctttaccgaa gtacgctaca aaggcactaa aaccatcgcc attacccctg actactctga
     3061 agtggccaaa ttgtgcgacc agtggctggc accgaaacaa ggcactgata gcgccctggc
     3121 gatggcaatg ggccatgtga ttttaaaaga gtttcatctc gataatccca gcgactactt
     3181 tatcaactac tgccgccgct acagcgacat gccgatgctg gtaatgctgg agcctcgcga
     3241 cgatggtagc tacgttcccg ggcgcatgat ccgcgcatct gacctggtgg atggactggg
     3301 cgaaagcaac aatccgcagt ggaaaaccgt agcagttaat accgcaggtg aattggtagt
     3361 gccgaacggt tcgattggtt tccgctgggg agaaaaaggc aaatggaatc tggaatccat
     3421 tgccgccggt acggaaaccg aattgtcgtt aaccctgctc ggtcaacatg acgctgttgc
     3481 aggcgtggcc ttcccctact ttggcggcat tgaaaatccg cattttcgca gcgtaaaaca
     3541 caatccggtg ctggtgcgcc aattgcccgt taaaaacctg acgttagtcg atggcaacac
     3601 ctgtccggtg gtcagcgttt atgatttggt actggcgaat tacggcctcg atcgcgggct
     3661 ggaagatgaa aacagtgcga aagattacgc tgaaatcaaa ccgtacaccc cagcctgggg
     3721 tgagcaaatt accggcgtgc cgcgccagta tattgaaacc atcgcccgtg aatttgccga
     3781 taccgcccat aaaacgcatg ggcgctcgat gattatcctc ggcgcaggtg ttaaccactg
     3841 gtatcacatg gacatgaact accgcgggat gatcaatatg ctgatcttct gcggttgtgt
     3901 cgggcaaagc ggtggcggct gggcacacta tgtcggccag gaaaaactgc gcccacaaac
     3961 cggctggttg ccgctggcct ttgcgctcga ctggaaccga ccaccacgcc agatgaacag
     4021 tacctcgttt ttctacaatc attccagcca atggcgctat gaaaaagtct ctgctcagga
     4081 gttactttca ccgctcgccg atgccagtaa gtacagcggt catctgattg atttcaacgt
     4141 tcgcgccgaa cgtatgggct ggctaccttc tgcgccgcag ctggggcgta acccgctcgg
     4201 gattaaagct gaagccgaca aggccggatt atcccccaca gaatttaccg cccaggcgct
     4261 gaaatcgggc gatttacgta tggcctgcga acaaccagat agcggcagca atcatccgcg
     4321 taatttgttt gtctggcgct ctaacctgct tggctcctcc ggcaaaggcc acgagtatat
     4381 gcagaagtat ctgctgggga ccgaaagcgg gattcagggc gaggaactcg gtgccagcga
     4441 cgggatcaaa ccggaagaag tcgagtggca aactgcggcg attgaaggca agctcgacct
     4501 gctggtgacg ctcgacttcc gcatgtccag tacctgcctg ttctccgata tcgttctgcc
     4561 caccgccacc tggtacgaaa aagacgatat gaacacctcg gatatgcatc cgtttattca
     4621 tccgctttct gcggcggtcg atccggcgtg ggaatcacgc agcgactggg aaatctacaa
     4681 aggtatcgcc aaagcatttt cgcaagtgtg cgtgggtcat cttggcaaag aaaccgacgt
     4741 ggtattacaa cccctactgc atgactctcc ggcagagctc tcacagccgt gtgaagtgct
     4801 cgactggcgc aaaggcgaat gcgatctgat tccaggcaaa accgcgccga atattgtggc
     4861 ggtggagcgc gactaccctg ctacgtatga acgctttacc tcgctcgggc cattgatgga
     4921 caaacttggc aatggcggta aagggatttc gtggaatacg caggatgaaa tcgatttcct
     4981 cggcaaactc aattacacca agcgtgatgg cccggcacag gggcgtccgc tgattgacac
     5041 cgccattgac gcttcagaag tgattctggc actggcaccg gaaaccaacg gtcatgttgc
     5101 agtcaaagcg tggcaggcgc tgggcgagat caccgggcgc gaacataccc atctggcgct
     5161 gcacaaagag gacgagaaga ttcgctttcg cgatattcag gcgcagccgc gtaaaattat
     5221 ctccagcccc acctggtccg gtctggaaag cgatcacgta tcctataacg cgggatacac
     5281 caacgttcat gagttaattc cgtggcgcac gctgtcggga cgccagcagc tctatcagga
     5341 tcatccgtgg atgcgtgctt ttggtgaaag cctggtggct tatcgcccgc ctatcgacac
     5401 ccgtagcgtc agtgagatgc gccagatacc gccaaacggc ttcccggaaa aagcacttaa
     5461 cttcctgacg ccgcaccaga aatggggcat tcactcaacc tacagtgaaa acctgctaat
     5521 gctgacgctc tctcgcggtg gaccgattgt ctggatcagc gaaacagatg cccgagaact
     5581 gaccattgtc gataacgact gggtggaagt attcaacgcc aacggcgcgc tgactgcccg
     5641 cgcggtggtc agccaacgtg taccgccggg catgaccatg atgtatcacg cccaggaacg
     5701 cattatgaat attcctggtt cggaagtaac tggcatgcgc ggcggcattc ataactcggt
     5761 cacccgcgtt tgcccgaaac caacgcatat gattggcggt tacgcgcagc tggcctgggg
     5821 ctttaactac tacggcaccg tcggatcgaa ccgcgatgag ttcatcatga tccgcaagat
     5881 gaagaacgtt aactggctgg atgatgaagg tcgcgatcag gtacaggagg cgaaaaaatg
     5941 aaaatccgtt cacaagtcgg catggtgctt aacctcgaca aatgtatcgg ctgccatacc
     6001 tgttcggtga cctgtaaaaa cgtctggacc gggcgcgaag gcatggagta cgcatggttt
     6061 aacaacgtcg aaaccaaacc gggcattggt tatccgaaaa actgggaaga tcaggaagag
     6121 tggcaaggcg gctgggtccg cgatgtgaat ggcaagatcc gtccgcgtct gggcagcaag
     6181 atgggcgtaa taaccaaaat cttcgccaac ccggtggtgc cgcagattga tgattactac
     6241 gaacctttca ccttcgacta cgaacatttg catagcgcac cggaaggcaa acatatccct
     6301 actgctcgcc cgcgttcact gattgacggc aaacggatgg acaaagtgat ctggggccca
     6361 aactgggaag aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg caacttcgag
     6421 gccatgcaaa aggagatgta cgggcagttt gaaaacacct tcatgatgta cctgccgcgc
     6481 ctgtgcgaac actgcctcaa tcccagttgc gtggcgacct gcccaagcgg cgctatctac
     6541 aaacgcgaag aagacggcat tgtgctgatt gatcaggata aatgccgtgg ctggcgtttg
     6601 tgcattagcg gttgtccgta caaaaaaatc tacttcaact ggaaaagcgg caagtcagaa
     6661 aaatgtatct tctgttatcc gcgaattgag tcaggccaac ccaccgtgtg ctcagaaacc
     6721 tgcgtgggtc gcatccggta tctgggcgtg ctgctttacg acgccgaccg cattgaggaa
     6781 gcggcgagca ccgagcgcga agttgacctc tatgaacgtc agtgcgaagt attcctcgat
     6841 ccacacgatc cctcagtgat cgaggaagcc ctgaaacaag gtattccaca aaacgtgatt
     6901 gaagctgccc agcgttcgcc tgtctacaaa atggcgatgg actggaaact ggcgctaccg
     6961 ctgcaccctg aatatcgcac cctgccaatg gtctggtacg ttcctccgct gtcgccgatt
     7021 cagtcctacg cagatgcggg cggtttgccg aaaagcgaag gcgtgctgcc cgccatcgaa
     7081 agcctgcgta ttccggtgca atatctcgcc aatatgttga gtgccggcga taccggtccg
     7141 gtactgcggg cgctgaaacg gatgatggcg atgcgccact atatgcgttc acaaaccgtg
     7201 gaaggcgtta ctgatactcg tgccatcgac gaagtaggcc tgagcgtcgc ccaggtcgaa
     7261 gagatgtatc gttacctcgc cattgccaac tatgaagatc gttttgtcat cccgacgagc
     7321 catcgggaaa tggcgggcga tgccttcgca gaacgcaacg gctgcggttt tacctttggc
     7381 gacggttgcc acggttcgga cagtaaattc aacctgttca acagtagccg tatcgatgcc
     7441 atcaacatca ccgaagtgcg cgacaaagcg gagggcgaat aatgcagatc ctcaaagtga
     7501 tcggcctgtt gatggagtat ccggacgagc tgttgtggga atgcaaggag gacgcgctgg
     7561 cgttgatccg cagcgacgcg ccgatgctta cggatttcac ccacaacctg cttaacgcgc
     7621 cgctgctgga taaacaggcc gaatggtgcg aagtgtttga ccgtggacgt accacatcgc
     7681 tgctgctgtt cgaacatgtt catgccgagt cccgcgatcg cggccaggca atggtggacc
     7741 tgctggcgga gtatgaaaag gtcggcctgc agctggattg tcgggaactg cccgattatc
     7801 taccgctgta tctggagtat ttaagcgtac tgcctgacga tcaggcgaaa gaaggattgc
     7861 tcaatgttgc gccgatcctc gccctgcttg gcggtcgctt aaaacaacgc gaggcaccgt
     7921 ggtacgcgtt gtttgatgct ctgctgcaac tggcgggaag ctccctttca agtgacagtg
     7981 tcacaaaaca agtaaacagc gaagagcgcg atgacacccg ccaggcgctt gatgcagtgt
     8041 gggaagagga acaggtgaag tttattgaag ataacgccac ggcgtgtgac agctcgccgc
     8101 ttaatcaata tcagcgacgc tttagccagg atgtcgcgcc gcaatatgtc gacatcagtg
     8161 cgggaggtgg gaaatgattc agtatctgaa cgtctttttt tacgatatct acccgtacat
     8221 ttgtgcgacg gtgtttttcc tcggcagctg gctgcgctac gactacgggc agtacacctg
     8281 gcgcgcctcc tcaagtcaaa tgctcgataa acgcgagatg gtgatatggt cgaatctgtt
     8341 ccatatcggc attttgggga ttttcttcgg gcacttgttc ggcatgttaa cgccgcactg
     8401 gatgtacgcg tggtttttgc cagtggcagc gaaacaacta atggcgatgg tgctcggcgg
     8461 tatttgcggt gtgttgacat tgattggcgg cgcaggactg ctgtggcgca ggctgacaaa
     8521 tcagcgcgtg cgtgcaactt ccactacgcc ggatattatc atcatgagca ttttgctgat
     8581 ccagtgtctg ctggggctaa gtaccatacc gttttcagca cagtatcctg atggtagcga
     8641 aatgatgaag ctggtgggtt gggcgcaaag cattgtcact ttccgtggtg gatcgtcaga
     8701 aatgcttaac ggcgtagcgt tcgttttccg cctgcatctg gtattgggaa tgaccatctt
     8761 cctgctcttc ccgttcacca gattggtgca tgtgtggagc gcgccgtttg agtactttac
     8821 tcgtcggtat cagattgtgc gttcgcggcg ataatttcac cctggccgga tagcccttcg
     8881 ctctccggcc acttgcaggc tatgatagtt ctccagtcac aggagaacgt taatgaaacc
     8941 gcaggtgtac cacgtcgatg cttttacctc tcaaccattt cgcggcaatt ctgccggtgt
     9001 tgttttcccc gccgataatc ttagcgaagc gcaaatgcag cttatcgccc gcgagttagg
     9061 ccattcggaa actgcttttc tgctgcacag cgacgacagc gatgtgcgca tccgctactt
     9121 tacgccaacg gttgaagtgc cgatttgcgg tcacgcgaca gtagctgcgc actatgtacg
     9181 tgcgaaggtg ttaggtttag gaaattgcac gatctggcaa acatcgctgg caggaaaaca
     9241 tcgcgtgact atcgaaaagc acaacgatga ttatcgtatt tcgctggaac aaggtacgcc
     9301 gggctttgag ccaccgctgg aaggtgaaac acgtgaggcg attatcaacg cgcttcatct
     9361 cactgaagac gatattttgc cgggcttgcc aattcaggtc gcaacaacag gacactcaaa
     9421 agtgatgatc ccgttgaaac cggaagtgga tatcgacgcc ctttcgcctg atcttgctgc
     9481 gctgagcgct atcagcaaac agattggctg caatggtttc ttcccgttcc agatccgccc
     9541 aggcaaaaac gaaaccgatg gtcgtatgtt ctcgcctgcg attggtattg tggaagatcc
     9601 ggtgactggc aatgccaatg gcccgatggg tgcatggttg gtacatcaca acgtattgcc
     9661 ccacgatggc aacgtgttat gtgttaaagg ccatcagggg cgcgcattgg ggcgtgacgg
     9721 catgattgaa gtgacggtga caattcgtga taaccaaccg gaaaaagtca ccatttcagg
     9781 cacagcggtg attttgtttc atgccgaatg ggcaattgaa ctctgaaacc gttgcctgat
     9841 gcgcttcgcc tgtcaggtct acaaccgagc taatgtatgc cggataaggc gtttacgccg
     9901 catccggcaa cctgacataa attattttcc cgcctccggg tgcgtatcaa acgccgccat
     9961 caccagcgct aactcatcca cggtaaagcc atgtttcgca tcatccacgc ccagaccaaa
    10021 ctgttcttgc atcacagcat ataatgacgc cacatccggt agatttcgct gctccaccgc
    10081 gtgcccattt tcataatggg taaaatgaaa attggtcagc gttagcttgc caccgtccgg
    10141 caaatggcgg cacatcagca aatgatggcg aaaatgcgac tgcggccagt gcgccgacca
    10201 gaaattgccc atcacataat cgctttgttg ctgctcgcag agatcaaaac ggtacatcga
    10261 ctgccagtgc tgatgatgat taaactgcaa cacccaatca tcaccctcct gcaacaaccg
    10321 ataatctccg tgtggcgtgg tctgcacgag atcggaaact aaacgaatcg gcgcggttag
    10381 cgtctgccca ccgaaaccga catcagcaat ccatttttcc tcttccagtt ccaccaacag
    10441 caaacgatgg gtgcgcggcg gtaatgctgg cggatttgat aacactacgc gccctaacaa
    10501 gctgcgaacg ttaaactcca gctcgcgtaa cacccgctca aacacgccat tctgctcaaa
    10561 acagtaaccg ccacgacggg ctatcaccag tttctcttcc agcgattgat catcaagctg
    10621 tatttccctc ggcagcaaaa cgtcgaggtt ttcaaacgga atagtgcaat tgtgtttcag
    10681 gtgcaatgca cgaagcgtat caatattgac cgcagcagct cccgaccagt taatacgggc
    10741 aaaatagtga ttcagaatgg gcgtcatcag gcgtttccct tagtggtttt ctcaacttat
    10801 aacctggaat agacgccgca ccggctattt tgtgcgatcg cggacatgca tcacgtttgc
    10861 aaatcgccaa tcccgttaat ttcgttactt attgcgttac ggactacgct taaaaaaacc
    10921 ttcttcgcga ggcgatacca tgagccgatt catccccata gaattacacc atgccagccg
    10981 tctgttaaat catggcccaa ctgtcatgat caccagtttc gatgagcaat cccagcggcg
    11041 taacattatg gcagcagcct ggtcaatgcc ggtggagttt gaaccgccac gcgtggcgat
    11101 tgtggtagat aaatcgacat ggaccaggga gttgattgaa cgtaacggta aatttggcat
    11161 cgttatcccg ggcgttgcag caactaactg gacgtgggca gtgggaagcg tgtcgggtcg
    11221 tgaagaagat aaatttaatt gctatggcat tccggtggtg aaaggtccgg tgcttggttt
    11281 gccacttgtt gaagagaaat gcctggcgtg gatggagtgc cgcctgctac ctgcgacttc
    11341 tgcgcaacaa aaatacgaca cgctgtttgg tgaggtggta tcagcagcgg cagacgcacg
    11401 ggtatttgtc gaaggccgct ggcagtttga tgatgataag ctcaatacgt tgcatcattt
    11461 aggtgctggc acgtttgtta ccagcggcaa gcgagttacg gcgggttaag cattacatgc
    11521 tgtaaccggg tttctttatc aaagcctcca tttggggcgc gatttcggca tcccagatag
    11581 cttgccaaga ttctggttga atctgctgta gagcaatgct tatcgagcta tctttactgt
    11641 tcagatggcg aataataacg tcggtaatat ctgcagcaag tgctgctttt tattgttcgt
    11701 ccagttcacg cggaaaacat ttaatgtcga tgtgcggcat gattgtgctc cttttgaaag
    11761 atgtcagtaa aattaacaca cattgtgaat gtcgtgtgtc cggtaatgcg tttttacgac
    11821 aataatgcca gggcaagatt acgaaagcct
//
LOCUS       DAZGLJ010000140        11782 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000140 DAZGLJ010000000
VERSION     DAZGLJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.140"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..596)
                     /locus_tag="ACHK49_003331"
     CDS             complement(<1..596)
                     /locus_tag="ACHK49_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO9263933.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(667..2781)
                     /gene="fusA"
                     /locus_tag="ACHK49_003332"
     CDS             complement(667..2781)
                     /gene="fusA"
                     /locus_tag="ACHK49_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO9263934.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="ACHK49_003333"
     CDS             complement(2878..3348)
                     /gene="rpsG"
                     /locus_tag="ACHK49_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO9263935.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="ACHK49_003334"
     CDS             complement(3445..3819)
                     /gene="rpsL"
                     /locus_tag="ACHK49_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO9263936.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="ACHK49_003335"
     CDS             complement(3945..4232)
                     /gene="tusB"
                     /locus_tag="ACHK49_003335"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO9263937.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="ACHK49_003336"
     CDS             complement(4240..4599)
                     /gene="tusC"
                     /locus_tag="ACHK49_003336"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO9263938.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="ACHK49_003337"
     CDS             complement(4599..4985)
                     /gene="tusD"
                     /locus_tag="ACHK49_003337"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO9263939.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4985..5707)
                     /gene="yheO"
                     /locus_tag="ACHK49_003338"
     CDS             complement(4985..5707)
                     /gene="yheO"
                     /locus_tag="ACHK49_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9263940.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="ACHK49_003339"
     CDS             complement(5874..6686)
                     /gene="fkpA"
                     /locus_tag="ACHK49_003339"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO9263941.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6907..7125
                     /gene="slyX"
                     /locus_tag="ACHK49_003340"
     CDS             6907..7125
                     /gene="slyX"
                     /locus_tag="ACHK49_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO9263942.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="ACHK49_003341"
     CDS             complement(7174..7764)
                     /gene="slyD"
                     /locus_tag="ACHK49_003341"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO9263943.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGFLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7859..8059)
                     /gene="yheV"
                     /locus_tag="ACHK49_003342"
     CDS             complement(7859..8059)
                     /gene="yheV"
                     /locus_tag="ACHK49_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO9263944.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="ACHK49_003343"
     CDS             complement(8069..9874)
                     /gene="kefB"
                     /locus_tag="ACHK49_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO9263945.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="ACHK49_003344"
     CDS             complement(9874..10425)
                     /gene="kefG"
                     /locus_tag="ACHK49_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO9263946.1"
                     /translation="MSQPAKVLLLFAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10556..>11782
                     /locus_tag="ACHK49_003345"
     CDS             10556..>11782
                     /locus_tag="ACHK49_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9263947.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQK"
BASE COUNT         2893 a         3249 c         2845 g         2795 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcgtactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcatat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cacgagcagc accgccgtag
      481 gttttagcca gtacggtggt gattgcagcg gtcagagtag ttttaccgtg gtcaacgtgg
      541 ccgatagtac caacgttaac gtgcggtttt gtacgttcaa atttttcttt agacacggct
      601 atattcctta ctatagtgct ctccccttca ggagagagca cgggactttg gtattaaccc
      661 ttaggcttat ttaccacggg cttcaattac ggcctgagca acgttactcg gcgcttcatc
      721 atacttcagg aattccatag tgtatgatgc acgacctttg gtcagagaac gcagctgagt
      781 tgcgtatccg aacatttcag acagcggtac ttcagcgtgg atcttaacgc cagtaacttc
      841 agattcctga cctttgagca taccacgacg acggctcaag tcaccgataa cgtcaccggt
      901 gttctcttcc ggagtttcta cttcaacctt catgatcggc tcaagcagaa ctggtttcgc
      961 tttcttaaag ccttctttaa aggcgataga agcagccagt ttaaacgcca gttcagagga
     1021 gtcaacgtca tggtaagaac cgaagtgcag acgaataccc atgtctacta ccgggtagcc
     1081 tgccagcgga cctgctttca gctgttcctg gataccttta tcaacggccg ggatgtattc
     1141 gccagggatt acaccacctt taatgtcgtt gatgaactcg tagcctttcg ggtttgaacc
     1201 cggctccagc gggtacatgt cgataacaac atgaccatac tgaccacgac caccagactg
     1261 tttcgcgtgt ttaccttcaa catcggtaac tttctggcgg atagtttcac ggtaagcaac
     1321 ctgcggttta cctacgttcg cttcaacgtt gaattcacgc ttcatacggt caacgatgat
     1381 gtcgaggtgc agttcgccca tacccgcgat aatggtctgg ttagattctt cgtcagtcca
     1441 tacacggaaa gacgggtctt ctttagccag acggcccaga gccagaccca ttttttcctg
     1501 gtcagctttg gttttcggtt caactgcgat ggagattacc ggctcaggga attccatacg
     1561 ttccagaatg atcggcgcat ccgggtcaca cagggtgtca ccagtggtta cgtctttcag
     1621 accgatagca gcagcgatgt cgcccgcgcg aacttctttg atctcttcac gtttgttagc
     1681 gtgcatctga acgatacgac cgaaacgctc acgtgcagct ttcacggagt tcagtacggt
     1741 atcaccagag ttaaccacac cggagtaaac acggaagaag gtcaggttac caacaaacgg
     1801 gtcggtagcg attttgaacg ccagtgcaga aaacggctcg tcatcacttg cgtgacgctc
     1861 agccggagtg tctttaccgt cgtccaggat accgttgatc gcaggtacgt caaccgggga
     1921 tggcaggtaa tcaattaccg catccagcat cgcctgaaca cctttgttct tgaacgcaga
     1981 accacaggtt accaggatga tttcgttgtt cagaacgcgc tgacgcagag cacctttgat
     2041 ttctgcttca gtcagttctt caccacccag gtatttttcc atcagctctt cagaagcttc
     2101 agctgcggat tcgatcaggt tctggtgcca ttcgttagcc agttcaacca tgtctgccgg
     2161 gatatcttcg tattcgaagg ttacgccctg gtcagcgtcg ttccagttga tagctttcat
     2221 tttcaccagg tcaacaacac cggtgaaatg ttcttcagca ccaatcgcca gctgcagcgg
     2281 aaccgggttc gcgcccagac gggttttgat ctggttaaca actttcagga agttcgcacc
     2341 catgcggtcc attttgttaa cgaacgcaat gcgcggaact ttatatttgt ttgcctgacg
     2401 ccatacggtt tcagactgcg gctgaacacc accaactgcg cagtaaacca ttaccgcacc
     2461 atcgagaaca cgcatggaac gttctacttc gattgtgaag tcaacgtgcc ccggggtgtc
     2521 gatgatgttg atgcgatgcg gctcatactg cttagccata ccagaccaga atgcagtagt
     2581 cgcagcggaa gtgatggtaa taccacgttc ctgctcctgc tccatccagt ccatggttgc
     2641 agcgccgtca tgaacttcac cgattttatg gtttacaccg gtgtagaaca gaatacgttc
     2701 ggtagtagtg gttttaccgg cgtcgatgtg cgcactgata ccgatattac ggtagcgtgc
     2761 gatgggtgtt gtacgagcca tttgtttcct cgtttatctt ttaggcgttc aatttaagta
     2821 gcccaaagcg ggctgcttac tggaagcgcc cgcctggtga ctaaaactcc gaagggatta
     2881 ccaacggtag tgtgcgaacg ccttgttggc ttcggccata cggtgaacgt cttcacgttt
     2941 cttaactgca gtacctttgt tttctgcagc atcagaaagt tcgttcgcca ggcgcagagc
     3001 catggattta tcaccgcgtt tacgagcagc ttcaacgatc caacgcattg ccagagcatt
     3061 acgacgaacc ggacggactt caactggtac ctgataagta gaaccaccaa cgcggcgaga
     3121 cttaacttct acagtcgggc gcacgttttc gagagctact tcgaatgctt ccagttcaga
     3181 tttaccagag cgctgagcca gggtctccag cgcgctgtat acgatagatt cagcagtaga
     3241 ttttttacca tctaccatca ggatatttac aaatttagcc agcagttctg atccgaactt
     3301 cggatccggc agaattttac gctgaccaat gacgcgacga cgtggcatgg aaatactccg
     3361 ttgttaattc aggattgtcc aaaactctac gagtttagtt tgacatttaa gttaaaacgt
     3421 ttggccttac ttaacggaga accattaagc cttaggacgc ttcacgccat acttggaacg
     3481 agcctgctta cggtctttaa cgccggagca gtcaagcgca ccacgtacgg tgtggtaacg
     3541 aacacccggg aggtctttaa cacgaccgcc acggatcagg atcacggagt gctcctgcag
     3601 gttgtgacct tcaccaccga tgtaggaagt cacttcgaaa ccgttagtca gacgaacacg
     3661 gcatacttta cgcagcgcgg agttcggttt tttaggagtg gtagtatata cacgagtaca
     3721 tacgccacgt ttttgcgggc atgcttccag cgcaggcacg ttgcttttcg caactttgcg
     3781 agcacgtggt ttgcgtacca gctggttaac tgttgccatt aaatagctcc tggttttagc
     3841 ttttgcttcg taaacacgta ataaaacgtc ctcacacaat atgaggacgc cgaattttag
     3901 ggcgatgccg aaaaggtgtc aagaaatata caacgatccc gccatcacca ggccatctgg
     3961 ctggagtgct taaccgtaag tctgacgaaa tcagtatagt caatgggaat gatgtcgttc
     4021 gaaatttgac cagtcaaacc gcgggcaata aggtcttcgt tcagggcata gaccttaatg
     4081 ggggcattac gcagactttc aaggtagcgg ttaccgtcaa ctgcggcagt tacgccatct
     4141 tgcaataata gcagttcgtc tccttcactg agcagacgca gcagcgcagc aaaatccgtc
     4201 agccagggtg aacgatgtaa cgtgtgcagc ataaacgcct caaaacctca aaataacatc
     4261 gtagttggcg agttcgcggc gtaaggcatc tgcttcgagc ggcgtggctt cgacaacaaa
     4321 gggtgtctgc ggatctaacc cgcgttcgcg cagtgaagcc gcacaaaccc agcactgttc
     4381 aatgtcgtac agacccaaca atttaaaagt ggcaatgtaa tcacgcgcca gcactgcatc
     4441 gggcttttgt cctgacagca gctgaaaaac gccatcagca ataaagaaga cggccagatc
     4501 gtcagttaat gcggaagttg ccagtaaagc atctaaacct tcccgaccag cagctgtacc
     4561 atgaggtgca gtagaaaaaa caaacgcaat tcgtttcatc agaactgtac caccctgtca
     4621 caggtcagcg aggcttccgc cagcgcgcca agtccgctta aggtaaatcc cggctgaagg
     4681 tttgacgaag ccagccccag ccttcctgct tcagtttcat cgacgatacc gcggcgtaac
     4741 gctgccgcta cgcagatatt cagcgccaca ccatgttgcg cattcaactg ttgccagccg
     4801 cgcacaaggt caaattcgtc acttgccggt gaggtcaatt ggttagcgtt atagacccct
     4861 tcccgataga aaaagacgct gcttaactca tggccttcta ctatcagcgc ctgcgcaaac
     4921 tgaaaagcac tactcgcctg ttgcgtaccg tatgctggcc cggtcaccac gatggcaaaa
     4981 cgcattactt atcttgcccc tggaaatcac cgcttttgaa ctggcggatg tagagataga
     5041 cagtgtgttt ggagatgttc aggcggtcag caacctggtt gatcgcgtct ttaatatcga
     5101 agatcccttt ctcgtagaga ttcagcacga tctgacgatt tttggcgtta ttagaaacat
     5161 tgcgatcggc attcacttct tcgatggtga actccagcgt ttgggtaacc agatcttcaa
     5221 cagaagaggc aaagttgacg cttgaaccga catccggggt ttctggcggc acaaaagtgc
     5281 tcataatctg cgagaaggga acatcaagat tcatattgat gcacagcaga ccaattacac
     5341 gctgttcgcg gttacgaatc gcgatagtca gggacttcat taatacgccg cttttggcgc
     5401 gagtaaagta gcatttagaa acgctgctat ccgctcccgt catatcgtgc agcatacgta
     5461 gcgccaggtc agtaattggc gaaccaatct tccggcctgt atgttcaccg ttagcaatgc
     5521 gaatggctga acattttaga tcctgcaaag agtgcaaaac gatttcacag tgggagccaa
     5581 taagcatcgc taacccgtcc accaccgctt cgtaggattt cagaatatca aaatcggtct
     5641 ggtcgaaagg acgttgatcc agtaaatcca actcactggt ttcgttggtt aaaagcgacc
     5701 tggacatgaa aaaaaacact ccttttcagg agcctgtcgt taacttttca gggcaggctc
     5761 attaatgatg cgggtaacta aattaataca gcggaggttc cgctttccag cactaattat
     5821 atccggcctg taataaaaaa accgccgcct ggtcaggcgg cggttcttaa tgcttatttt
     5881 ttagcagaat ctgcggcttt cgcatcagct tccggctttg catcagcctt cggcgctggt
     5941 ttcacatcca gcagctctac gtcaaacacc agggtagaat tcggtgggat ccccggaaca
     6001 cccgctttgc cgtaagccag ttctggtgga ataaccagtt tgatcttacc gcctttcttg
     6061 atgttcttca gaccttctgt ccaacccggg ataacaccgt ccagacggaa agaaagcggt
     6121 tcaccacggg tgtaagagtt gtcgaactct ttaccgtcga tcagcgtacc tttgtagttc
     6181 actacaacag tatcgctgtc tttcggtgct tcgcctttac cggcttctac tacctgataa
     6241 accagaccag ttgaagaggt tttcacacct ttctctttgg caaatttctc gcggtactct
     6301 ttaccttttg cttcgttatc agccgcgtct ttttccatct tcgcctgagc agaagacttc
     6361 acgcgagctt cgaatgcttg cagagtctgt tcgatctctt ggtcggagag tttgctctta
     6421 tcagcaaatg catcctgaac accagcgatc agctgatctt tatccagttt gatgcccagt
     6481 ttttcttgtt ctttaagaga gttttccatg taacgaccca gcgaagcacc cagtgcataa
     6541 gctgatttct gatcgtcatt tttgaacgct gctttgctgt cagcagttgt agcaggtttt
     6601 gcagcttcag cagcaaaagt gattggtgca tgcagggcaa cggccattgt ggtcgccagc
     6661 agcgttactt taaacagtga tttcatccat atctccagga tcggggcatc tcaccccagg
     6721 gttaactatt atcagaaggg tactataaag cgttgtcgaa caaatctaca tacagacacg
     6781 ccctattatc atctattttc agactctttt tgtttaaatt agtttcgatg accgcgaaat
     6841 gagtgctgtc tcgggcagca aagttaagta gaatccgcgg cgaccattcg acaaaagagg
     6901 tgaatcatgc aggatttatc attggaagca cgcctggcag agctggagag ccgactggct
     6961 tttcaggaaa tcaccattga agaactgaac gtcacggtga ccgctcatga aatggagatg
     7021 gcgaaactgc gcgatcatct gcgtctgctg accgagaagt taaaagccag ccagccgtcg
     7081 aacatcgcgt cgcaggctga agaaacgcca ccgccacatt attgaggcgt aaaaaaagcg
     7141 gggattcccc gcttttttgt caccttttcg gtattagtgg caaccacaac cgccgttgcc
     7201 tttaccgcca cagcagcctt cgccaccgtg ttcatgaccg tgatcgtggc catgaccgcc
     7261 gcagcaaccg tcgtggtcgt gatcgtggtg atgatcgtgc gcgccgtgaa cgtggccatg
     7321 agccagttct tcttcggtcg cttcgcgaat cgccacaact tcaacgttga atttcaggtt
     7381 ctgaccggcc agcatgtggt taccatcaac cacgacgtga tcgtcttcaa ccgcagtgat
     7441 ttcaaccggt accggcccct ggtcggtttc agccaggaaa cgcataccta cctgcagttc
     7501 atcaacgccc ataaatacgt ctttaggaac acgttgcacc aggttttcgt cgtactgacc
     7561 gtaagcgtcg ttcgcgccaa cagcgacatc aaatttgtcg ccaacttcat gaccttccag
     7621 cgccgtttcc aggccagaga tcaggaaacc gtgaccatgc aggtagtcca gcggcgcact
     7681 caccggagac tcatcaacca acacaccgtc ttctgtacgt acctgatagg ccaggctgac
     7741 caccaggtct tttgctactt tcatgatatc tcctgagcat gggaagaata gtggcgcaga
     7801 ttgtagcgga attctgcagc cgtgtactca ctagcttaaa aaaactcggc gcatatcgct
     7861 agtccggatg aaaaatcccg atcacttgct catctttgcg aacgtgatcg cgggcttctt
     7921 tgtctgcttc tcgcatctga tgtccgcact taacacattc aacaatatca atattatttt
     7981 cgcgccacat cgccattgaa tcctgcgcct gacaggccgg gcattttgcg cccgcgataa
     8041 aacgttttcg gattgccatc tttaccctct actcaaattc atcccagccg tccagctggc
     8101 gtcgttcttg ttgcatttca cgctggaaaa tctcttccag ttcgcgtcgg gcttccctgg
     8161 cgcgagaaat ttgtacggta tcagcatgca tcgggattaa ctcccgcagc attcgcatat
     8221 ccaggcggcg aaaatgcagt tgcgcccgct gtgcctgatg cggatgcatg ccaagcgtga
     8281 ccagcgtctt gcgccccagc tctaacgcac tggagaatgt ttcacgggaa aactgcgtca
     8341 cccctgcctg taataactca tgcgcttcca cacgtccgcg cgctcgcgca agaatatgca
     8401 aatgcggaaa gtgctgctgg catatttcca ccagcttcat ggtgtcttcc ggctcgttac
     8461 aggtaatgac gatagactca gcggcctctg cacccgcaga acgtaaaaga tcgacctgcg
     8521 tggcgtcgcc gtaataaact ttgtagccat atttacgcat caggttaacg gcgctgatat
     8581 cccgctccag cacggtaatg cgcattttat ttgccatcag caaacgacca atcacctgac
     8641 caaaacgccc gaagcccacg acaatgacct ggggtttatc atcgttgacc cacggttttt
     8701 cgtcttcttc ttccggtccg ttaaactggc gggatagcca tttatccacc agcttcatca
     8761 gcaacggcgt ggtcatcatg gaaagcgtca ccgtcaccag caacagcgcc atctggtcgc
     8821 cctggaataa acgttgtgaa gaagcggtag aaaagaggac aaaggcaaac tcaccgccct
     8881 gactcaacac gccagcaaac tgcatccgct ctgagctacg cacgccatac aatcgcgcca
     8941 gcagatacag cacgagaatt ttcaccgcca ccagcacaac cacgcttatc actacccaca
     9001 acagatgggt ataaagcacc ccgaggttga gcgacatgcc gacagagata aagaacaaac
     9061 cgagcagcaa tcctttgaag ggatcgatag ccgtttccag ttcatggcga tattcacttt
     9121 ccgccagcag cacgcccgca ataaatgtac cgagcgccat cgacagcccc agcgcatcca
     9181 taaacaatgc ggaacccaac accagcagca gcgtcgcggc ggtgaacact tcccgcacgc
     9241 cagaagctgc aataaagcgg aataccggac gcagtaaata gcgcccacca atcagcatgc
     9301 cgacaaacgc cagcaccttc atgccgatct tcatccagtc gaaatgttcg tctgccgaac
     9361 ccgccaacaa cggcactaac gccagtgccg ggatcaccgc cagatcctga aacagcagta
     9421 ccgaaaaccc gagctgcccg gattcgctgc gattcatccc tttctcacgc atcaattgca
     9481 acgccattgc ggtcgaagac atcgcaaggc caatgccacc gaccaccgcc gcctgccagg
     9541 cgaaatccgt cagcatcaat aatcccgcca gcaacgccgc gcttaacagc acctgtgccg
     9601 cgcctacgcc aaaaatcgaa cgccgcagtt gccaaagctt ggagggattc aactcaaggc
     9661 cgatgataaa catcaggaat accacgccga gttccgaaaa gtggaggatc tcatcgacgt
     9721 cgctaataaa ccccagcccc cacgggccaa ttgcaatccc tgccagcaaa tatcccaaca
     9781 cagcgccaat ccccagccgc gatgccaacg gcaccgcagc caccgccgcg aagagaaaca
     9841 gcactcctgc gagtaaaaaa tcggaacctt ccatcagcgg cctcctggag acagcggatt
     9901 tgccagccag tcaccgtagg ctctggcgtg gctcgccagc tcctgtgcgc tttgccgtct
     9961 cgcccagtaa ataatgatgg gacttaacca atgcatccgg cacatgcccg ccgccagttc
    10021 aaagggacgc agaacgtcgc tcatggggta gcgattcaat gcgtcataac ggtaagcact
    10081 ttccggctcg ccggtggtga tcacgctacg ccagtacttt cccgccagtt ggtttcctcc
    10141 cggcccgctg gcaaaaccac ggcttaatac ccggtccagc cactctttca gtagcgccgg
    10201 gcagctatag gtataaagag gatgctgaaa gacaatcacc tcgtgctcgc gcagtaatgc
    10261 ctgctcacgg gggatatcaa taaaaaaatc gggatagtgc gcataaaggt cgtgcacggt
    10321 aacattgctg agctgcgtgg ccggtttaag cagtacccgg tttgccaccg agtcctgaga
    10381 ttccggatgg gcaaacagca gcaaaacttt cgctggctga gacatcatcc ccctcccggt
    10441 atggtttttg tgtatagtcg ctgttttggg ctaccattgc gcccggtgcg gcagctcgcc
    10501 catacattac attatcataa tgataagtta acatagtctg aacatacggc accttatgat
    10561 tgttttctcc tcgttacaaa ttcgtcgcgg cgtgcgcgtc ctgctggata atgccaccgc
    10621 caccatcaac cctgggcaga aagtcggcct ggtgggtaaa aacggctgtg gtaaatctac
    10681 cctgctggca ttgctgaaaa atgaaatcag cgccgacggc ggcagctaca cctttccggg
    10741 aagctggcaa ctggcgtggg tgaatcagga aacgccggcg ttaccgcaag cggcgctgga
    10801 atatgtcatt gacggcgacc gtgaatatcg tcaactagaa gcgcagctac acgacgccaa
    10861 cgaacgtaac gacgggcacg ccattgcgac cattcatggc aagctggatg ctattgacgc
    10921 atggagtatt cgctcccgtg ccgccagcct gctgcacggc ctcggtttca gcaatgaaca
    10981 actggagcgc ccggtaagtg atttctccgg tggctggcgt atgcgtctta accttgccca
    11041 ggcgctgatt tgccgttcag acttgctgct gctcgacgaa ccgactaacc acctcgatct
    11101 cgatgccgtt atctggctgg aaaaatggct gaagagctat cagggcacgc tgatcctgat
    11161 ctctcacgac cgcgacttcc tcgatccgat cgtcgataaa attattcata tcgaacaaca
    11221 aagcatgttc gagtacaccg gcaactacag ttcgtttgaa gtacagcgcg ccacccgtct
    11281 ggcgcagcaa caagcgatgt acgaaagcca gcaggaacgc gtagcgcatc tgcaaagtta
    11341 tatcgaccgt ttccgtgcca aagccaccaa agcgaagcag gcccagagcc gcattaagat
    11401 gctcgagcgt atggagctaa ttgcccccgc gcacgtcgac aacccgttcc gctttagctt
    11461 ccgcgcgccg gaaagcctgc caaatccgtt actgaagatg gaaaaagtca gcgcgggcta
    11521 tggcgatcgc attattctcg actcgattaa actgaacctg gtgcccggct cgcgcattgg
    11581 tctgttaggc cgcaatggcg cgggtaaatc gacattaatc aaactgttag ccggtgaact
    11641 tgcgccagtc agcggtgaaa ttggtctggc gaaagggatc aaactcggct acttcgccca
    11701 gcatcaactt gaatacctgc gcgccgacga atcacctatt caacatctgg cacgtttagc
    11761 gccgcaggag ctggaacaaa aa
//
LOCUS       DAZGLJ010000141        11775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000141 DAZGLJ010000000
VERSION     DAZGLJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.141"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            84..872
                     /gene="phnC"
                     /locus_tag="ACHK49_003346"
     CDS             84..872
                     /gene="phnC"
                     /locus_tag="ACHK49_003346"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO9263948.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            897..1913
                     /gene="phnD"
                     /locus_tag="ACHK49_003347"
     CDS             897..1913
                     /gene="phnD"
                     /locus_tag="ACHK49_003347"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9263949.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            2019..2798
                     /gene="phnE"
                     /locus_tag="ACHK49_003348"
     CDS             2019..2798
                     /gene="phnE"
                     /locus_tag="ACHK49_003348"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO9263950.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRVINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            2819..3544
                     /gene="phnF"
                     /locus_tag="ACHK49_003349"
     CDS             2819..3544
                     /gene="phnF"
                     /locus_tag="ACHK49_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO9263951.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            3545..3997
                     /gene="phnG"
                     /locus_tag="ACHK49_003350"
     CDS             3545..3997
                     /gene="phnG"
                     /locus_tag="ACHK49_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO9263952.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            3994..4578
                     /gene="phnH"
                     /locus_tag="ACHK49_003351"
     CDS             3994..4578
                     /gene="phnH"
                     /locus_tag="ACHK49_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO9263953.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            4578..5642
                     /gene="phnI"
                     /locus_tag="ACHK49_003352"
     CDS             4578..5642
                     /gene="phnI"
                     /locus_tag="ACHK49_003352"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO9263954.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLVKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            5635..6480
                     /gene="phnJ"
                     /locus_tag="ACHK49_003353"
     CDS             5635..6480
                     /gene="phnJ"
                     /locus_tag="ACHK49_003353"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO9263955.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            6477..7235
                     /gene="phnK"
                     /locus_tag="ACHK49_003354"
     CDS             6477..7235
                     /gene="phnK"
                     /locus_tag="ACHK49_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO9263956.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMGEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            7346..8026
                     /gene="phnL"
                     /locus_tag="ACHK49_003355"
     CDS             7346..8026
                     /gene="phnL"
                     /locus_tag="ACHK49_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO9263957.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            8023..9159
                     /gene="phnM"
                     /locus_tag="ACHK49_003356"
                     /pseudo
     CDS             8023..9159
                     /gene="phnM"
                     /locus_tag="ACHK49_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
     gene            9159..9716
                     /gene="phnN"
                     /locus_tag="ACHK49_003357"
     CDS             9159..9716
                     /gene="phnN"
                     /locus_tag="ACHK49_003357"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO9263958.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            9703..10137
                     /gene="phnO"
                     /locus_tag="ACHK49_003358"
     CDS             9703..10137
                     /gene="phnO"
                     /locus_tag="ACHK49_003358"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO9263959.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            10139..10897
                     /gene="phnP"
                     /locus_tag="ACHK49_003359"
     CDS             10139..10897
                     /gene="phnP"
                     /locus_tag="ACHK49_003359"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO9263960.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(11044..11373)
                     /gene="yjdP"
                     /locus_tag="ACHK49_003360"
     CDS             complement(11044..11373)
                     /gene="yjdP"
                     /locus_tag="ACHK49_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO9263961.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(11442..11759)
                     /locus_tag="ACHK49_003361"
     CDS             complement(11442..11759)
                     /locus_tag="ACHK49_003361"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFO9263962.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
BASE COUNT         2504 a         3614 c         3378 g         2279 t
ORIGIN      
        1 cttcatctgt tagtcacttt taattaacca aatcgtcaca ataatccgcc acgatggagc
       61 cactttttta gggaggctgc atcatgcaaa cgattatccg tgtcgagaag ctcgccaaaa
      121 ccttcaatca gcatcaggcg ctgcatgcgg ttgatctgaa cattcatcac ggtgaaatgg
      181 tggctctgct tgggccgtcg ggttccggca aatccaccct tttacgtcac ttaagcggtt
      241 tgattaccgg cgataaatcc gccggcagcc atatcgagct gctgggccgc acagtccagc
      301 gcgaaggccg tctggcgcgc gatatccgca aaagccgcgc caacaccggc tacatcttcc
      361 aacaattcaa cctggtgaac cgcctgagcg tactggagaa cgtgctgatt ggcgcgctcg
      421 gcagcacgcc gttctggcgc acctgtttta gctggttcac cggcgagcag aaacagcgcg
      481 cgttacaggc gctgacccgc gttggcatgg tgcattttgc ccatcagcgc gtttccaccc
      541 tctccggcgg acagcagcag cgtgtggcga ttgcccgcgc gctgatgcag caggcgaagg
      601 tgattctggc cgatgaaccc atcgcctcgc tggacccgga atccgcccgc atcgtgatgg
      661 acaccctgcg cgacatcaat cagaacgacg gcatcaccgt ggtcgtcacg ctgcatcagg
      721 tggattacgc cctgcgctac tgcgaacgca tcgtcgccct gcgccagggg cacgttttct
      781 acgacggcag cagccaacag tttgataacg aacgttttga ccatctctac cgcagcatta
      841 atcgcatcga agagaacgcg aaagctgcct gacatcccca tcattgagga aaacgaatga
      901 acgctaagat aattgcctcg ctggccttca ccagcatgtt cagcctcagc accctgttaa
      961 gcccggcaca cgccgaagag caggaaaagg cgctgaattt cggcattatt tcaacggaat
     1021 cacagcaaaa cctgaaaccg caatggacgc cattcttaca ggatatggag aagaagctgg
     1081 gcgtgaaggt gaacgccttc tttgccccag actacgcagg cattatccag ggaatgcgct
     1141 tcaataaagt ggatatcgcc tggtacggca acctgtcggc aatggaagcg gtggatcgcg
     1201 ccaacggcca ggtcttcgcc cagacggtcg cggcggatgg atcgccaggt tactggagcg
     1261 tgttgatcgt caacaaagat agtccgatca acaacctgaa cgatctgctg gcgaagcgga
     1321 aagatctcac cttcggcaat ggcgatccta actccacctc tggcttcctc gtccccggtt
     1381 actacgtctt tgccaaaaac aatatctccg ccagcgactt taagcgcacc gtcaacgctg
     1441 ggcatgaaac caacgcgctg gccgtcgcca acaagcaggt ggatgttgcc accaacaaca
     1501 ccgaaaacct cgacaagctg aaaacctccg cgccagagaa gctgaaagaa ctgaaggtga
     1561 tctggaagtc gccgctgatc ccaggcgatc cgatcgtctg gcgtaaaaac ctttccgaaa
     1621 ccaccaaaga caagatctac gacttcttta tgaactacgg caaaacgccg gaagaaaaag
     1681 cggtgctgga acgcctgggc tgggcgccgt tccgcgcttc cagcgacctg caactggtgc
     1741 cgattcgcca gctcgcgctg tttaaagaga tgcagggcgt gaaaagcaat aaaggactga
     1801 atgagcagga caagctggca aaaaccaccg agattcaggc gcagctggat gacctggacc
     1861 gcctgaacaa cgcgttaagc gcgatgagtt cggtgagtaa agcggtgcag taaatcgtag
     1921 gtcggataag acgccccggc gtcgcatccg acaatgtgca ggcgttgatg ccggatgcgg
     1981 tgcaagcacc ttatccggcc tacagaccgg agccaaacat gcaaaccatc accatcgccc
     2041 cacccaagcg cagctggttc tcgcttctga gctgggccgt tgttctcgcc gtgttggtcg
     2101 tctcgtggca gggcgcggaa atggccccgc tcacgctgat taaagacggc ggcaacatgg
     2161 cgacgttcgc cgccgacttc ttcccgcccg atttcagcca gtggcaggat tacctcaccg
     2221 aaatggccgt cacgctgcaa atcgccgtct ggggcacggc gctggcggtg gttctctcca
     2281 tccccttcgg cctgatgagc gccgaaaacc tggtgccgtg gtgggtttac cagcccgttc
     2341 gccgcctgat ggacgcctgc cgcgtcatta acgaaatggt cttcgccatg ctgttcgtgg
     2401 tcgccgtcgg cctcggcccg ttcgctggcg tgctggcgct gtttatccac accaccggcg
     2461 tgctctccaa actgctttcc gaagcggtgg aagcgattga acccggcccg gtggaaggca
     2521 ttcgcgccac cggcgccaac aagctcgaag agatcctcta cggcgtgctg ccgcaggtga
     2581 tgccgctgct gatctcctac tccctctatc gcttcgaatc caacgtccgc tcggcgaccg
     2641 tcgtcggcat ggtcggtgca ggcgggatcg gcgtcaccct gtgggaagcg attcgcggtt
     2701 tccagttcca acaaacctgc gccctgatgg tgcttatcat cgtcacggtc agcctgctgg
     2761 atttcctctc tcaacggttg cgtaagcact ttatctgata agcgaggcat tgatatctat
     2821 gcacttgtct acacatccga ccagctaccc aacacgctat caggagatag ccgcaaaact
     2881 tgagcaggag cttcgtcaac actaccgctg cggcgactat cttcccgccg agcagcaact
     2941 ggcggcgcgc tttgaggtga accgccacac cctgcgccgc gccatcgacc aactggtgga
     3001 aaaaggctgg gtacagcgcc gtcagggcgt cggcgtgctg gtgctgatgc gcccgttcga
     3061 ttacccgctc aacgcccagg cgcgttttag ccagaacctg ctggaccagg gtagccatcc
     3121 caccagcgaa aaactgctgt cggtattgcg ccccgcgtcc ggtcacgtcg ctgacgcgct
     3181 ggggattacc gagggcgaga acgttatcca tttgcgcacc ctgcgtcgcg tcaacggcgt
     3241 cgcgctctgt ttaatcgacc actactttgc ggacctcacc ctttggccga cgctgcaacg
     3301 cttcgacagc ggttcgctgc acgattttct gcgcgagcaa accggaattg cgctgcgccg
     3361 cagccagacg cggatcagcg cccgtcgcgc ccaggccaaa gagtgccagc gtcttgaaat
     3421 tccgaatatg tcgccgctgc tgtgcgtgcg cacccttaac caccgtgacg gtgaaagcag
     3481 cccggcggag tactccgtca gcctgacgcg cgccgacatg attgaattca ctatggagca
     3541 ctgaatgcac gcagataccg cgacccgcca gcactggatg tccgtactgg cgcacagcca
     3601 accggctgaa ctggcagccc gcctgaaagc actgaacatc accgccgact atgaggttat
     3661 ccgcgccgcc gaaaccggcc tggtgcagat tcaggcgcgg atgggcggca ccggcgaacg
     3721 cttttttgcc ggcgacgcca cgctcacccg cgccgccgta cgcctgactg acggcacgct
     3781 cggctacagc tgggtgctgg ggcgcgacaa acagcacgcc gaacgctgcg cgctgattga
     3841 cgcgctgatg cagcaatctc gctattttca aaacttatca gaaaccctta ttgccccgct
     3901 ggacgctgac cgtatggcac gcattgccgc acgccaggcc gaagtgaacg ccagccgggt
     3961 cgacttcttt acgatggttc gcggagacaa cgcatgaccc tggaaaccgc ttttatgctt
     4021 cccgtgcagg atgcccagca cagttttcgt cgcctgttaa aggccatgag cgagccgggc
     4081 gtgattgtcg ccctgcatca gctcaagcgc ggctggcagc cgctgaatat cgccaccacc
     4141 agcgtgctgc tgacgctggc cgataacgac acgccggtgt ggcttgccgc gccattgagt
     4201 aacgatatcg tcagccagag cctgcgtttt cataccaacg cgccgctggt cagccagccg
     4261 gaacaggcga ccttcgcggt gacggatgag gcgatttcca gcgaacagct taacgccctt
     4321 tccaccggca ccgccgttgc gccggaagcg ggtgcaacgc tgattttaca ggtcgccagc
     4381 ctgagcggcg ggcgcatgtt acgtctcacc ggcgcgggca ttgccgaaga acgaatgatc
     4441 gctccgcagc tgccggagtg cattctgcac gaactcaccg agcgcccgca cccgttcccg
     4501 ctcggcatcg acctgatcct gacctgcggc gagcgcctgc tggctattcc gcgaaccact
     4561 catgtggagg tgtgctgatg tacgttgccg tgaaaggggg cgagaaggcg atcgacgccg
     4621 ctcacgccct gcaagagagc cgacgccggg gcgataccga tttgcctgaa ctgagcgtcg
     4681 cccagattga acagcagctt aatcttgcgg tggatcgcgt gatgaccgaa ggcggcattg
     4741 ccgaccgcga actggcggcg ctggcgctga aacaggccag cggcgataac gtcgaagcaa
     4801 ttttcctgct gcgcgcctac cgcaccacgc tggcgaagct cgcggtaagc gagccgctcg
     4861 acaccacaga gatgcgtctc gaacggcgta tttcggcggt ttataaagac attcccggcg
     4921 gccagctgct tggcccaacc tacgactaca cccatcgcct gctcgatttc accctgctgg
     4981 caaacggtga agcgccgacg ctgaccaccg ccgacagcga acaacagccg tcgccgcacg
     5041 ttttcagcct gctggcgcgt caggggctgg tgaagtttga agaagatagc ggcgcacagc
     5101 cggatgacat cacccgcacg ccgccggttt acccctgctc acgctcctcc cgcctgcaac
     5161 aattaatgcg cggggacgaa ggctatttgc tggcgctggc ctactccacc cagcgtggtt
     5221 acgggcgcaa tcacccgttc gcgggcgaga tccgcagtgg ttacatcgac gtgtcgattg
     5281 tgccggaaga gctgggattt gcggtaaacg tcggcgaact actgatgacc gagtgtgaaa
     5341 tggtcaacgg ttttatcgac ccgccggatg agccgccgca cttcacgcgc ggctacgggc
     5401 tggtattcgg catgagcgag cgcaaagcga tggcaatggc gctggtcgat cgtgcgttgc
     5461 aggctccgga atacggcgag cacgcgacag gcccggcgca ggatgaagag tttgtgctgg
     5521 cacatgccga caacgtcgaa gccgcaggct ttgtctcgca cctcaaactc ccccactacg
     5581 tcgatttcca ggccgaactg gagctactca aacgtctgca acaggagaag aaccatggct
     5641 aatctgagcg gctacaactt tgcctacctc gacgagcaga ccaaacgcat gatccgccgc
     5701 gctatcttaa aagcggtggc gatccccggt tatcaggtgc cgtttggcgg gcgcgagatg
     5761 ccgatgccat acggctgggg aaccggcggc atacagctca ccgccagcgt gattggcgaa
     5821 agcgacgtgc tgaaggtgat tgaccagggt gcggatgaca ccaccaacgc cgtgtcgatt
     5881 cgcaacttct ttaagcgcgt gaccggggta aacaccactg aacgtacgga cgatgcgacg
     5941 cttatccaga cgcgtcaccg catccccgaa acgccgctga ccgaagatca gatcattatc
     6001 ttccaggtgc caatcccgga accgctgcgc tttatcgagc cgcgcgaaac ggaaacccgc
     6061 accatgcacg cgctggaaga gtacggcgtg atgcaggtga aactgtatga agatatcgcc
     6121 cgcttcggtc atatcgccac tacctatgcc tatccggtga aggtgaacgg gcgctacgta
     6181 atggacccgt cgccgatccc gaaattcgat aacccaaaaa tggacatgat gcccgccctg
     6241 caactgttcg gcgcggggcg cgagaagcgc atctatgcgg tgccgccgtt tacccgcgtg
     6301 gaaagtctcg atttcgacga tcacccgttc accgttcagc agtgggatga gccatgcgcc
     6361 atctgcggat cgacccacag ctatcttgat gaagtggtgc tggatgacgc cggaaaccgc
     6421 atgtttgtct gctccgatac cgattattgc cgccaacaga gcgaggcaaa aaaccaatga
     6481 atcaaccgtt actttcggtc aataacctga cccaccttta cgcgccgggc aaaggcttta
     6541 gcgatgtctc ttttgattta tggccggggg aagtgctggg cattgtcggg gagtccggct
     6601 ccgggaagac cacgctgctg aaatcaatct ccgcgcgcct gacgccgcag cagggggaga
     6661 ttcactatga gagccgttcg ctgtacgcaa tgggcgaggc cgaccgccgt cgcctgctgc
     6721 gcaccgaatg gggcgtagtg catcagcatc cactcgacgg cctgcgccgt caggtctcgg
     6781 caggcggcaa tatcggcgag cggctgatgg cgaccggggc acgtcattac ggcgatattc
     6841 gtgccaccgc gcagaagtgg ctggaagagg tggaaattcc cgccaaccgg atcgacgacc
     6901 tgccgaccac cttttccggc ggcatgcagc aacgtttgca gatcgcccgc aacctggtga
     6961 cgcatccgaa gctggtgttt atggacgagc cgaccggcgg actggatgtg tcggtacagg
     7021 cacgcctgct cgacctgctg cgcggcctgg tggtggagct gaacctcgcg gtagtgattg
     7081 tcacccacga tttaggcgtc gcccgcctgc tggcggaccg tttgctggtg atgaagcagg
     7141 ggcaagtggt ggagagtggg ttaaccgacc gcgtgctcga cgacccgcac catccgtata
     7201 cacaactgct ggtgtcatcg gttttgcaga attgagccgg tgccggatgc ggcgtaaacg
     7261 ccttatccgg cctacaaatg cgctccccgt aggtcggata agacgcgtca gcgtcgcatc
     7321 cgacacccga accacgaggc gaaaaatgat taacgtacaa aacgtcagta aaaccttcat
     7381 cctgcaccag caaaacggcg tgcgcctgcc cgtcttacaa cgtgcctcgc tcaccgtcaa
     7441 cgcgggcgaa tgcgtggtgc tccacggcca ttccggcagc ggcaaatcaa ccttgctgcg
     7501 ctcgctgtac gccaactatc tgcccgacga agggcaaatc cagatcaaac acggtgacga
     7561 atgggtcgat ctggtcaccg cgccagcgcg caaagtggtg gagatccgca aaaccaccgt
     7621 cggctgggtg agccagtttc tgcgcgtcat cccgcgtatt tcagcactgg aagtggtgat
     7681 gcagccgctg ctcgataccg gcgttccgcg tgaagcctgc gccgctaaag ccgcgcgtct
     7741 gctcacccgc ctgaacgtgc cggaacgcct gtggcacctg gcgccatcaa ccttttccgg
     7801 tggcgaacag cagcgcgtca acatcgcccg cggctttatc gtcgactacc ccattctgct
     7861 gctcgacgaa cctaccgcct cgctggacgc caaaaacagc gccgccgtag tcgagctgat
     7921 tcgcgaagcc aaaacccgtg gcgcggccat cgtcggcatc ttccatgacg aagccgtacg
     7981 taatgacgtc gccgaccgct tgcacccaat gggagcctct gcatgattat caataacgtc
     8041 aaactggtgc tggaaaacga agtggtacac ggttcgctgg aggtgcagga tggcgaaatc
     8101 cgcgcctttg ccgaaagcca gagccgcctg ccagaagcga tggacggcga aggtggctgg
     8161 ctactacccg gcctgattga gctgcatacc gacaacctcg ataaattctt caccccgcgc
     8221 ccgtaagtcg actggcccgc ccattcggcg atgagcagcc acgacgcgct gatggtggca
     8281 agcggcatca ccaccgtact ggacgccgtg gcgattggcg acgtgcgcga cggcggcgat
     8341 cggctggaga atctggagaa gatgatcaac gccatcgaag agacgcaaaa acgcggcgtc
     8401 aaccgcgccg agcaccgcct gcacctgcgc tgcgaactgc cgcatcacac cacactgccg
     8461 ctgtttgaaa aactggttca gcgcgaaccg gtgacgctgg tgtcgctgat ggaccactca
     8521 ccgggccagc gccagttcgc taaccgcgag aagtatcgcg aatattatca gggcaaatac
     8581 tccctcaccg atgcgcagat gcagcagtac gaagaagagc aattggcgct cgccgcacgc
     8641 tggtcgcagc cgaatcgcga atccatcgcc gccctgtgcc acgcgcgaca aattgcgctc
     8701 gccagccacg atgacgccac gcacgcccac gttgccgaat cccaccaact tggcagcgtg
     8761 atcgccgaat ttcccaccac gttcgaagcg gcggaagcct cgcgtaagca tggcatgaac
     8821 gtgctgatgg gtgcgccaaa tatcgtgcgt ggcggctcgc actccggcaa tgtggcggcc
     8881 agtgaactgg cgcagcttgg tctgctggat atcctctctt ccgactacta ccccgccagc
     8941 ctgctggatg cggcgttccg cgtcgccgat gacgagagca accgctttac gctaccgcag
     9001 gcggtgaggc tggtgaccaa aaatccggcg caggcgctga atcttcagga tcgcggggtg
     9061 attggcgagg gtaaacgcgc tgacctggtg ctggcgcatc gccagggcaa tcacattcat
     9121 atcgaccacg tctggcgtca gggtaaaagg gtgttctgat gatgggaaaa ctgatttggt
     9181 taatggggcc gtccggctcc gggaaagaca gcctgctggc ggaactccgc ctgcgggaac
     9241 aaacgcagtt actggtggcg catcgctaca tcacgcgcga cgccagcgcc ggaagtgaaa
     9301 accatatcgc cctgagtgag cgggagtttt ttacccgcgc ggggcaaaac ctgctggcct
     9361 taagctggca cgctaacggt ctgtattatg gcgtcggcat cgagattgac ctctggctgc
     9421 acgctggatt cgacgtagtc gtcaacggtt cacgcgcgca tttgccgcag gcacgggcgc
     9481 gctatcaatc ggcgctgctg cccgtctgtt tacaggtttc gccggagatc ctgcgccagc
     9541 gcctggaaaa ccgtggccgt gaaaacgcca gtgaaattaa cgcccggctg gcgcgcgccg
     9601 cccgctatac tccacaggat tgccatacgc tcaacaatga cggcagcctg cgccagtcgg
     9661 tcgacacact gctgacgctg attcatcaga aggagaaaca ccatgcctgc ttgtgagctt
     9721 cgcccggcca cgcagtacga taccgacgcg gtttacgcgc tgatttgtga gctaaaacag
     9781 gcggagtttg accaccacgc gtttcgcgtg ggctttaacg ccaacctgcg cgacccgaac
     9841 atgcgctacc atctggcgct gctggatggc gaagttgtcg gcatgatcgg cctgcatttg
     9901 cagtttcatc tgcatcatgt caactggatc ggcgaaattc aggaactggt ggtgatgccg
     9961 caggcgcgcg gcctgaacgt cggtagcaag ttgctggcgt gggcagaaga agaagcccgc
    10021 caggccgggg ccgaaatgac cgagctttcg accaacgtga agcgccacga cgcgcaccgt
    10081 ttctatctgc gagaaggcta cgagcagagc cacttccgct tcaccaaggc gctgtaacat
    10141 gagcctgacc ctcacgctca ccggcactgg cggcgcacag ggcgttccgg catggggctg
    10201 cgagtgcgcg gcctgcgcca gagcgcggcg ctcgccgcag tatcgccgcc agccatgcag
    10261 cggcgtagtg aagtttaacg acgcgatcac cctgatcgac gccgggcggc acgatctcac
    10321 cgatcgctgg tcgcctggat cgttccagca gtttttgctg acgcactacc atatggatca
    10381 cgtccagggg ctatttccgc tacgctgggg cgtgggcgat gtgatcccgg tctacggccc
    10441 gccggatgaa cagggctgcg acgatctgtt taaacatccg ggcctgcttg atttcagcca
    10501 cacagtggaa ccgtttgtgg tgtttgattt gcaggggttg caggtcacgc cgctgccgct
    10561 taaccactca aaactgacct tcggttatct gctggaaacg gcacatagcc gggtggcgtg
    10621 gctgtctgac accgctggcc tgccggaaaa aacgctgaaa tttttactca ataatcatcc
    10681 gcaggtgatg gtgattgatt gcagtcaccc gccgcgcgcg gatgcaccgc gtaatcactg
    10741 tgatttaaat accgtgcttg cgctgaatca ggttatccgc tcgccacagg tgattctgac
    10801 ccatatcagc caccagtttg atgcgtggct gatggaaaac gcactaccgt cagggtttga
    10861 ggtggggttt gatgggatgg agattggggt ggcgtgatga gagggaatgt gcgcgctggc
    10921 cccctcaccc taaccctctc cccagagggg cgaggggacc gattgtgctc aatattgaat
    10981 attgcgctcg ttttctccct ctccccattg gggtgagggg cgatgcctgc tccataccca
    11041 acctcatcgc ccatactcat cttccattct ccgctcttca tcctccagtt gccgacgctc
    11101 ctgatcaagc tggcgctggc gatcgtccag ctgcctgcgg cgatcttcaa actggcggcg
    11161 gcggtcgtca tattgtctgc gccgatcgtc gctcacttca cgctgccagc cgtcgtcgcg
    11221 cgaatcttca tagtctcgcc cacggtcagg gttataagcg tcattaatcg cctgctgaat
    11281 attaccaata gtatcgtcga taatatcagc ctgggccgga acggtggaca gcgtgagcag
    11341 ggtgaataaa agaaatagcg gaaagcgttt cattagccaa cctcaaaaag aaactctatc
    11401 cacattagtc attactcatc catgcaagta gtggatgaat ctcaatttct ccgctgctct
    11461 attgccgtaa tcgcctccac gcgttgttga tgacgaccgc cttcgtactg tgcgcccagc
    11521 cacgcatcca caatcatttt tgccagttcg aggccaacca ctcgtgaacc aaaagccagt
    11581 gcaacttcac tggcataatg cggataatca gtgcgctctg acgacctggt gtctttatca
    11641 atcacctcac tcattgacca tgaataaaag gctggcacag caacacagaa tcctgcggtg
    11701 ccagcgacat gaccgcccat tgctccggta cgttttttaa aaagaaacac cggttgcatt
    11761 gataaattgt gctca
//
LOCUS       DAZGLJ010000142        11697 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000142 DAZGLJ010000000
VERSION     DAZGLJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.142"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..263)
                     /locus_tag="ACHK49_003362"
     CDS             complement(<1..263)
                     /locus_tag="ACHK49_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263963.1"
                     /translation="MVDASAQPHIVVTLEPNPWAAFYFDINIANTGNAPAYNVEVVFD
                     PPLVNAEHREKSEIPFSKVSVLKNGQSLTSNLCKYEQIKDQIY"
     gene            364..1534
                     /locus_tag="ACHK49_003363"
                     /pseudo
     CDS             364..1534
                     /locus_tag="ACHK49_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1617..1874
                     /locus_tag="ACHK49_003364"
     CDS             1617..1874
                     /locus_tag="ACHK49_003364"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263964.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHKDIIDNNRSINWPV"
     gene            complement(2432..3199)
                     /gene="fecE"
                     /locus_tag="ACHK49_003365"
     CDS             complement(2432..3199)
                     /gene="fecE"
                     /locus_tag="ACHK49_003365"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFO9263965.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(3200..4156)
                     /gene="fecD"
                     /locus_tag="ACHK49_003366"
     CDS             complement(3200..4156)
                     /gene="fecD"
                     /locus_tag="ACHK49_003366"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFO9263966.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(4153..5151)
                     /gene="fecC"
                     /locus_tag="ACHK49_003367"
     CDS             complement(4153..5151)
                     /gene="fecC"
                     /locus_tag="ACHK49_003367"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFO9263967.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILASIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(5148..6050)
                     /gene="fecB"
                     /locus_tag="ACHK49_003368"
     CDS             complement(5148..6050)
                     /gene="fecB"
                     /locus_tag="ACHK49_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFO9263968.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(6095..8419)
                     /gene="fecA"
                     /locus_tag="ACHK49_003369"
     CDS             complement(6095..8419)
                     /gene="fecA"
                     /locus_tag="ACHK49_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFO9263969.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLSPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(8506..9459)
                     /gene="fecR"
                     /locus_tag="ACHK49_003370"
     CDS             complement(8506..9459)
                     /gene="fecR"
                     /locus_tag="ACHK49_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFO9263970.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(9456..9977)
                     /gene="fecI"
                     /locus_tag="ACHK49_003371"
     CDS             complement(9456..9977)
                     /gene="fecI"
                     /locus_tag="ACHK49_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFO9263971.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            10114..10182
                     /gene="ykiE"
                     /locus_tag="ACHK49_003372"
     CDS             10114..10182
                     /gene="ykiE"
                     /locus_tag="ACHK49_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFO9263972.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            10269..10966
                     /locus_tag="ACHK49_003373"
                     /pseudo
     CDS             10269..10966
                     /locus_tag="ACHK49_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
     gene            complement(10977..11048)
                     /locus_tag="ACHK49_003374"
                     /pseudo
     CDS             complement(10977..11048)
                     /locus_tag="ACHK49_003374"
                     /inference="COORDINATES: protein motif:HMM:NF013446.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11111..11257
                     /locus_tag="ACHK49_003375"
                     /pseudo
     CDS             11111..11257
                     /locus_tag="ACHK49_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11284..11388
                     /locus_tag="ACHK49_003376"
                     /pseudo
     CDS             11284..11388
                     /locus_tag="ACHK49_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2744 a         3103 c         3216 g         2634 t
ORIGIN      
        1 ttataaattt gatctttgat ttgttcatac ttacagagat tgctggtaag tgattgccca
       61 ttttttaaca cgcttacctt actaaacgga atctcacttt tttctctatg ctccgcattt
      121 actagtggag gatcaaacac aacctcaaca ttataggcag gtgcgttccc tgtattagca
      181 atgttaatat caaagtaaaa agcggcccaa gggtttggct caagtgttac aactatatgg
      241 ggttgagctg atgcgtcaac cattcttttt gtctcgtctg ctctgaagtg gtgaagtggt
      301 ttactgaatt tggccacctg aacagaggtg atatgctcac ctcagaacaa cacaggtgct
      361 ccaatgaaaa aaagaaattt tagcgcagag tttaaacgcg aatccgctca actggttgtt
      421 gaccagacat acacggtggc agatgccgcc aaagctatgg atgttggcct ttccacaatg
      481 acaagatggg tcaaacaact gcgtgatgag cgtcagggca aaacaccaaa agcctctccg
      541 ataacaccag aacaaatcga aatacgtaag ctgaggaaaa agctacaacg cattgaaatg
      601 gagaatgaaa tattaaaaaa ggctacaacc gcgctcttga tgtcagactc cctgaacagt
      661 tctcgataat caggaaactc agagcgcatt atcctgtggt cacactctgc catgtgttcg
      721 gggttcatcg cagcagctac agatactgga aaaaccgtcc tgaaaaacca gacggcagac
      781 gggctgtatt acgcagccag gtacttgaac tgcatggcat cagccacggc tctgccggag
      841 caagaagcat cgccacaatg gcaacccgga gaggctacca gatggggcac tggcttgctg
      901 gcagactcat gaaagagctg gggctggtca gttgccagca gccgactcac cggtataagc
      961 gtggcggtca tgagcacgtt gctatcccga atcatcttga gcgacagttc gccgtaacgg
     1021 aaccaaatca ggtgtggtgc ggtgatgtga cctatatctg gacgggtaag cgctgggcgt
     1081 acctcgccgt tgttctcgac ctgttcgcaa gaaaaccagt gggctgggcc atgtcgttct
     1141 cgccggacag caggcttacc atgaaagcac tggaaatggc atgggaaacc cgtggtaagc
     1201 ccgtcggggt gatgttccag gcgatcaagg cagtcattat acgagcaggc agttccggca
     1261 gttactgtgg cgataccgga tcaggcagag tatgagtcgg cgtggaaact gctgggataa
     1321 cagcccaatg gagcgcttct tcaggagtct gaagaacgaa tgggtgccag cgacgggcta
     1381 tgtaagcttc agcgatgcag ctcacgcaat aacggactat atcgttggat attacagcgc
     1441 actaagaccg cacgaatata atggtgggtt accaccaaac gaatcagaaa accgatactg
     1501 gaaaaactct aacgcggagg ccagttttag ttgaccacaa cagactacct gaagggagcc
     1561 gcggtcgcct agcagttgca gtagcaggag atcatccagc cgcagtacag gtcacgatga
     1621 ctctggttaa tgataccggc tttgaccccg tattttccgg ctctatcgct gaatcatggc
     1681 gtcagcagcc gtgcacacca tcctattgtt gtgactggga ggctgccacc atgcttcgcg
     1741 ctttccctct ggcgaaaaag ggagaaggac gggcccgtct gccttcactt tatgccagct
     1801 tcggtaagct gggtgagaca ccgactcata aagatatcat tgataacaat cgatccatca
     1861 actggcctgt ataacgtggc tgccggtgat taagaaagct gcacctacct aagtagtagc
     1921 aaacgcacac tttttagaaa aatcgatggt cagaaactgg attagcaatt ccgttccagg
     1981 gttgcttttg atttacgttg gcgtctgatc attgatttat cctcaaaagc ccaacctcat
     2041 tggtaatgaa ccagctccgt gaatgtccgc tctggcacag agcgaaattt tttgatctcc
     2101 ccccctgaaa tctaaactta gtcatgtcac gtttttgggt ttctaaaatt ttaacttcgc
     2161 gtttttcgtt gccgtaaggg ttatacagaa atgtccgtta agcagagttc aaaattgatt
     2221 gccgtgatca cgactggttt gaaagccgcg cccaagcctg tacagctctg gtttgcgttg
     2281 attatgaacc tgtcagccta aagcaagcgg atggacgatg agtattggta atctttcaga
     2341 gtccggaaaa gttcagcccc agtctgaaca ggcttgctgg cgccagtcca gtttcattca
     2401 gtcgtggttt ggttcttacg gcctgtgcaa tctacctcat taggcacatc ggcctgccag
     2461 ataccggctc ggggtgtatt tccgcttcca cgctgaatac tgttctcagc aatcctgggg
     2521 tcatcacctc ttctggtgtg ccttgcgcca taacatgtcc gtttgccatt accaccagtt
     2581 gatcgcagta ccggctagcc tgattaaggt cgtgcagcac agcgaccacc gttttcccct
     2641 gagtccggag ttcgcccatc aaccgcatca ggtccacctg gtgattgata tcaagatagg
     2701 tggttggctc atcaagtaat acaacgggcg tattctgggc caggaccatc gccagaaatg
     2761 cgcgctggcg ctgaccgccg gaaagctcgg ttaaccgacg aacggcaaga tgattgatcc
     2821 gggtctggtt catggcgaca ttaactcgtg cattgtcttc agcggagaga cgcccccaga
     2881 gtgacagcca gggattacga ccatacgaaa ccagctcctg gactgtgatc ccctctggcg
     2941 ttaaatggtg ctgaggcagc agcgaaagcc tgcgggccaa ctggcgcgat gagagcatat
     3001 ttatgggatt atcgccgaga aatacggtgc cagactgcgg cattaaaagc cgcgaaaaac
     3061 agtttaacag cgtcgatttc ccgcaaccgt taggaccgat cagggcggtg atcttccccg
     3121 ttggcagtga gagtgaaacg tcgttaagta ccttgtctgt cccgtaactg accgtcagat
     3181 tttcagttcg taaagtcatt tatcgcattc tcacaagcaa ccagacaaac cacggcgcac
     3241 cgataatggc ggtcagcacg ccaaccggga gctccagtgg gggatgaata attctcgcca
     3301 gcagatcggc aaccaccaac agcaacgcac ctgtcagggc tgaaacaggc agcagtctgc
     3361 ggtgacgtcc accggtgatg ctacgcatca tatgcggcac cacgagacca ataaagctaa
     3421 tcgggccgca ggcggccacg ccggtagatg tcatggcgac agctagtaac aaagcccaga
     3481 atcgggtatg gggcaccgac acaccgagcg tggtggcgcg cgcatcgccg agtgcaagga
     3541 ggtcgagatc gcggcaaaaa ctcaggctca gcggcagaaa taaaatcatc agcgggatgg
     3601 caatcttcac aaagctccag tcacggcccc ataagctgcc ggtcagccac agcagggcgt
     3661 tgttcacatc ctgtgggcgc gagagcatca gataatccgt caggctggcc cagcatgcag
     3721 aaagcgccac gccggtgagc gccagcttca tcggctggtg ggtctttgcc agcatcttca
     3781 gtaatatcaa ccccgccatg ccgcccgcaa aggccagcag cggcagcacc atcacgggca
     3841 gtgacggcat aagaagtaga gcccccacag aggccaggct ggcggcatgg ttaacgccga
     3901 gaatatccgg tgatgccaga gggttgcgca caatcccctg tatcagcacg cccgccacgg
     3961 cgagggctgc accgacaaac agtgccagca gcaagcgcgg cagtcggtac tccatcaata
     4021 cataataatg ctcgcgtccg gcctgccagt cggtcagcag cgcgcgccac ggcacgggga
     4081 tcactcccat atggagtgat aacagcgcac agcccgccag ggcaagggtg atgaaaataa
     4141 ccagcgcaat tttcatcctc gcctcctcac aagccagaca aagcaagggc tgccaatcag
     4201 cgccagcact gcgcctgcgg gcagatctcc ggggaaggcc agcgcgcgtg cgagtacatc
     4261 tgccagcagc atcagcgtgg cccccagcag catgctcact ggcagtacgt tgcgctgatc
     4321 gaagcctgcc cagaagcgcg ccagatgtgg caccagcaga ccgataaacg ccaccggacc
     4381 tgccacgctg acgcacgcac caaccagaag cagcactaac atattgatga ccaaacgtag
     4441 cctcgtcagg ttcactccca gcgtatgggc ggtgctgtcg ctgaggttga gcaggttcag
     4501 ttgattcgcc agcagcaaca cgacagggac tgcagtgacc accaccggca agagctgcca
     4561 gacatcctgc cagcgggcgt gggacactcc gcctgccagc cagtaaaaga tgccgtaagc
     4621 atgatcttcg gccagcagca gggtgatgcg ggtaaggccc atacaaaagg ccgacagcgc
     4681 gatactcgcg aggatcagtt tgtttctgtc atgggtatga cgaaatccgc ctcctgcggt
     4741 catgaccagc agccagctca cgccgccccc gcatgccgcg atgaacgaca gagaatagcc
     4801 tgcaatcggc gtcggactca gcgcgctggt aagcgccata gccagcgccg cgccgctgtt
     4861 aatgccgagc agtgaaggag aggccattgg gttgtgggtc agggtttgca gcagcgtgcc
     4921 cgcgagcgcc aggcttgcgc cgatcagaac ggcgaccagg cttcgtggca aacgaaggtt
     4981 ttgcaccagc gcttctggta gcgttggcgt gtgtccaggc agcagggcgc gggttgcatc
     5041 tgctccggaa acaggaatgg ccgagtagca aaacagactc agccagaaaa taataataag
     5101 tgctgcaacg ggaagccccc acagcagcac cgggtgtttt atcgcggtca tttcacaacg
     5161 gtaagcggct gatggtggaa gatttttacc gtgtcagcgg caatacgctc tgcagcaaaa
     5221 ataccgcgca tccgcgccca ggtgttactg tcgaccgaag caacctgctg cttctgcgcg
     5281 gcggttaaca tctgccagag cggatcttgt tgccagcgtt taacaatgct ctcttcgcga
     5341 tagtgggcaa ccagcagcca ggcaggattg accgccagca gttgctccag gccgatggac
     5401 ggcatggacg cacccgccat cgcagcggga acgttcagcc ccagagaggc cagcacgctg
     5461 ccggtccagg tctcctgagt atgcaggttg aattgctgtt cgcgtgatgt gccaaaggcc
     5521 acgcgtgtcc ctttgggaag ctggctggcc cactgcgcca tcctctcttt atgttgttcc
     5581 aggcgtgcct gcatctctcg ctttttaccc accatttcgc cgatgatagc cgcagattgc
     5641 aaattttcag cgtaggtttc gttgcgggac ttaagcagca gtaccggcgc gatttgctgc
     5701 aaggcgatgt aaacccccgc atggcgactg ctgtcggcaa tgatcaggtc tggtttcaga
     5761 gcggcaatgg cttccaggct cggctgcgcg cgcgttccga cggactgcca cggtttcagg
     5821 tgcgcacgca cttcgggcag gatgcgtttt gcatcgttat cgtcggcaat accgatcggg
     5881 ctgacgtcca cggcggccag cgcatcggcg aacgagagtt ccagcaccac aatccgttgt
     5941 ggcgtttttt cgagtgtaaa cgtgccgtgt tcgtcctgaa ccgtggcggc aaaggcgtgg
     6001 ctgatcacca gcagcaggcc tgcaaaaaca aaacggataa atgccaacat aatcacattc
     6061 cagctaaaag cccggcaagc cgggcgttaa cacatcagaa cttcaacgac ccctgcatat
     6121 acagcgtgcg cggctggcct gcatagatgc ctttgttgtt gtcgtcataa gagcggatga
     6181 agtagtcctg gtcgaagatg tttttcacac cgaatgccag gttcagatct gccatctgcg
     6241 ggccaaaatc atacgccacg cgtgcgcccc agagcatgaa gccgggaatg cggccggtac
     6301 tgccgtcggc gctctctttc accgtattgg cgttatccgc aaactggctg gactggaaat
     6361 cgctgttcag attgaacgtc cagtttcctg gcttgtagtc cacgcccagc gtgcctttat
     6421 gtttcgggga gaatggtacc agattgccgt aggtgtcgcc tttctcgcgg atttccgcgt
     6481 tcacatacgc atagctggcg tagatggaaa cgttatcaag cgttggcgtt agcgtaccca
     6541 gatcgtaacg tgcctgcgtt tccagcccgg tatggcgcgt tttgccacgt gcagtgacgg
     6601 tgtcgttggt ctggttggag tcgtactgat tgttaaagtt aatcaggaac agccccattt
     6661 ccgccgtcag cgcgccgtcg tcgtagcggg taccgagttc ccaggttcgc gctttttccg
     6721 gttccacatt gccgctttgc acagccttgc caatctggct gtactgtacg gtgccgaacg
     6781 agccttcagt gtttgcataa agattccagc tgtcagtcag gtgatagagc acgttcaacg
     6841 ccggaagcgg tgcgttatag ctcacttctt cgtgcgtgcc tgtgatggcg ttgttctggt
     6901 atgactcgat atgttcgaaa cgcatacccg gcgtgatggt ccagttgccg atgtcgattt
     6961 tgtcatccag ataccaggcg tgcgcctcgg tgccggaacg cgtatcgcgg tcgtaagggc
     7021 ttgagccgga cggcaactgc ccgctgctgg tggcggtgta gtaacgcatt tcatgcgttg
     7081 attcattcaa atagcgatag cccacgccca cttcgtgcgc ggaagggccg atcataaaga
     7141 tctggctgta gcgtggctca ataccgcgca cccagtagtt acgcggcgag agggtgatgc
     7201 gtttgccttg ctccaggtag ccgctgcgca gggtttgggt gtagaacccc tgaatgttga
     7261 atttatgctg gctgtctggc tggaactggt agcccaggct cgccagcttg cgacgacccc
     7321 agaagcggtc atacgggcgg gtggattgcc agcgatcggc gtcgtaatcc gcgcgagaca
     7381 ggccaccggg catgtcggct tcaccatcgt aatattgcag caggctgttg aaggtgtgca
     7441 cctcatccgg cgcatatttg cttttcagca tcaggtcgtc gatgcgggtg gcgctgtgct
     7501 cgcgccagtc actgccgcgc gtgccggagt agagcagcgc ggtgccaaaa ccgttgtccg
     7561 ctgtgccgcc caccatcagg ttgtgcgtct ctttcgggtt gttttgtgaa gaggttgggc
     7621 tgagctgacc ttccacgccc gcctcgatac caaagtcctg cggaatggca cgggtaacaa
     7681 agttcaccac gccgcccacg ctctgcggtc cgtaacgcac cgcaccacca ccgcgtacca
     7741 cgtcaatggc atccatgttg ccgagcgaaa cgggagccag tgaaagctgc ggctgaccgt
     7801 aaggggcgaa ggggacgggg atgccgtcca tcaggacggt cgagcggctg gcgaggcgcg
     7861 ggctcaggcc ccggatgcca aagttcatcg ccaggtcgtg gctgccggtg ccgttgtttt
     7921 ccggcgcgct gacgccaggg atgcggttaa gtacctcacg catggtggtt gcgccggttt
     7981 tggcgaaatc ctcacggcgg atcacgtcac gcgcgccagc atgttcaaat acgtcgtttt
     8041 cacgcgcatc acccagccag tcgccgacca cggtcagggc atcttctttt ggtgcgggcg
     8101 cgggctccag cgtccagctg ttatttccca gcggttttac ctgcagtccg ctgccgtcca
     8161 gcagttgttg caggccgctc tcgacgtcgt aatcgccgtg caggccgttg ctctgcttgc
     8221 cgcgcgtcag gctggcgtca accgagaggg taaatccgct gtgtgcggca tactgattga
     8281 gcgctttatc gagcgatccc ggtgcgatat taacctgtgc agcaaaagcg gaaaacgaga
     8341 gaccggccag cggcagcagg ctcaggcgaa tggcgttaac caaaggtgtt gttttacgaa
     8401 aaacgcgtaa cggcgtcata ccttccccat catcattttt gttgtgttca gctatgagtc
     8461 gaacgagaag ggtaaaaagg acaatcgaaa taagaattat tttccttaca gtggtgaaat
     8521 gtttatccag taccgcgtaa tagactgaat tttaacggga agcgtttgcg cgataacgtt
     8581 caggatcgca tcggtatttt tcagcgggaa cgtcccgctc aggcgcagcc cggcaacggc
     8641 gggatcgcag cgcagcacgc cgttgcggta acgggttagc gtggctatca cctcacccag
     8701 cggtttatcg ctgaagctca ggatgtcctt cgtccagctt gtactctcgt catccagcgg
     8761 tttcactgcg ccaaactcag aggcgctgaa ctgcaggctt tcaccagcgt tcacgatgcg
     8821 tttttgcgcg ggggcactgg cgagaagcac ttccacagcg tgctgctgca cgtcaagctg
     8881 cgtgaaatta tcctgctggc ggacggtaaa ttctgtccct aaagcagtga gctggccctg
     8941 acgggtcagg acacggaagg ggcgttgcag ggcatctttc gcggtagtaa tggcgatttc
     9001 accgtaccag agccggacgg tgcgctgatg cgcatcaaaa cgcacatccg ccgcgctttg
     9061 ggtattcagc gtgagcaggg agccatcttc cagttgctga cggctgactg tgcctttggc
     9121 ggtgcggtaa tctgcccgca gaccttcgcc ggtttccgac tgccagagct gccagcctcc
     9181 gccagcgccg agcaacagca gtaatccttt catcacgtga cggcgggtga ggcgggtatc
     9241 gtgcaacgcc ctgctggcta cgtcgccagg cacaccgcca agctggttgc gcaggttttc
     9301 aacctgctgc caggcccact ggttatcctg atcctgttca taccactgtt gccagcgcgc
     9361 ttcctgttgt gggctgacgc gctcgccgct tagcacggca taccagtggg aagctgaacg
     9421 cagcgcctga cggcgggaat cggttaacaa aggattcata acccatactc cagacggaac
     9481 agcaggcagt gctcgacggc tttcgccacg tattttttca cggagctgat ggaaacaccg
     9541 agtttgtgcg caatctcgct gtatgtcaga ccatccagtt gcgaaagcag aaacgcttca
     9601 cgtgttttgc cgtttagccc gtccagcatg ctgtcgagga gttgtagggt ctcgagttgg
     9661 ctttcgcgtt cctcaggtga aggcgctccc ccctccggca taagcgccag catctccaga
     9721 tacgcttttt ccagcgcgtt tcggcgaaac aggtccacca tcacgcgttt ggcgatagtg
     9781 cagaggaagg agcgaggatc gcggatcgtc gagagcgttt cgctgaccat tacccgcaaa
     9841 aaagtgtcct gggcaatgtc atctgcatca aaagcagact ggagtttgcg cgtcagccag
     9901 cttttcaacc agccgtgatg tgtgccataa agcgactcga acgttaagga agctgtggta
     9961 gtggcgcggt cagacatgcg gagtgcatca aaagttaatt atcacgtagt catattaata
    10021 tgagaatggt tatcattaca attggaaata aaattgtttc caatagacat ttttaacatg
    10081 ttgtttttct aagtgttata aggtaggtat aaaatgggat ggagcctctg cttctggcat
    10141 gtgtcggtca gaatgactca tgatgtggtc tgctattatt gacatcctca ctgccctaaa
    10201 ggatggggat ttcggtaatg ctgccaactt actgatttag tgtatgatgg tgattttaag
    10261 gtgcttgcgt ggcttccatt tccatcagat gtccttcctg ctccgctact gaaggcgtgg
    10321 tgcgtaacgg caaaagcact gccggacatc agcgctatct ctgctctcct tgccgtaaaa
    10381 catggcaact acagttcact tacaccgcct ctcagcccgg taagcaccag aaaatcattg
    10441 atatggccat gaatggcgtc ggatgtcgcg ccagtgcacg cattatgggc gttggcctca
    10501 acacggtttt acgtcactta aaaaaatcag gccgcagtcg gtaacctcgc gcatacaacc
    10561 gggcagtgat gtgattgtct gcgctgaaat ggacgaacag tggggctacg tcggtgctaa
    10621 atcacgtcag cgctggctgt tttacgcgta tgacaggata cggaggacgg ttgtggcgca
    10681 cgtcttcggt gaacgcactc tggccacact ggagcgtctt ctgagcctgc tgtcggcctt
    10741 tgaggtcgtg gtatagatga cggatggctg gccgctgtat gaatcacgcc tgaagggaaa
    10801 gctgcacgtt atcagcaagc gttacactca gcgcattgag cgacataatc tgaatctgag
    10861 acaacatctg gcaaggctgg gacggaagtt actgtcgttc tcaaaatcgg tggagttgca
    10921 tgacaaggtc atcgggcatt atctgaacat aaaacactat cagtaagttg gagtcattac
    10981 ctcctcccga actcaaagct atcatacaga taattactgg ttaaccctat atagggcatt
    11041 ttaaagtgtt gtgatcttac ccagcaatag tggacacgcg gctaagtgag taaactctca
    11101 gtcagaggtg attcacaaga caaaacagtt tcaaccagta aaaaaacgta aacaccattc
    11161 gcatgaattt cgtagttaag atctgaagct tgcagaatgc atcggtgtta ctgccggagt
    11221 tcgtgaactt agtctgcatg aatcaccaca gctttacatc tgtcgcagta aacagcaaaa
    11281 tcagcaaact tacttaatta tatcgagtgc gattacaatc gctggcgatg gtacagttgg
    11341 tgtggcggtc tcagtccgga acaatttgaa aatcagaatc tcacttaaaa cggcatccat
    11401 tttacaaaag atggccgtct gtgtatttgt aatgaatacc ttctacatgt ttagagtgca
    11461 taatccacga caaggcgata tcctaaaaat tagctagcag gcaactttga tggcatagat
    11521 aatgttttta gtaaaaaaga gttgactgaa tataatacag cctcttccta cataagaagg
    11581 aatgtgctca caaaaaaaaa ccattagtct gcttcctgtt cttacaataa tcagtattat
    11641 gtattttatt tatatagcaa aatatgcttg ttacacctgt cataattagg catggta
//
LOCUS       DAZGLJ010000143        11450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000143 DAZGLJ010000000
VERSION     DAZGLJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.143"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            130..747
                     /gene="yijF"
                     /locus_tag="ACHK49_003377"
     CDS             130..747
                     /gene="yijF"
                     /locus_tag="ACHK49_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO9263973.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(774..1679)
                     /gene="yijE"
                     /locus_tag="ACHK49_003378"
     CDS             complement(774..1679)
                     /gene="yijE"
                     /locus_tag="ACHK49_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO9263974.1"
                     /translation="MSVAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(1773..3953)
                     /gene="katG"
                     /locus_tag="ACHK49_003379"
     CDS             complement(1773..3953)
                     /gene="katG"
                     /locus_tag="ACHK49_003379"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO9263975.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(4282..5172)
                     /gene="metF"
                     /locus_tag="ACHK49_003380"
     CDS             complement(4282..5172)
                     /gene="metF"
                     /locus_tag="ACHK49_003380"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO9263976.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            5248..5382
                     /locus_tag="ACHK49_003381"
     CDS             5248..5382
                     /locus_tag="ACHK49_003381"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9263977.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(5521..7953)
                     /gene="metL"
                     /locus_tag="ACHK49_003382"
     CDS             complement(5521..7953)
                     /gene="metL"
                     /locus_tag="ACHK49_003382"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO9263978.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPVSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(7956..9116)
                     /gene="metB"
                     /locus_tag="ACHK49_003383"
     CDS             complement(7956..9116)
                     /gene="metB"
                     /locus_tag="ACHK49_003383"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO9263979.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVMTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            9393..9710
                     /gene="metJ"
                     /locus_tag="ACHK49_003384"
     CDS             9393..9710
                     /gene="metJ"
                     /locus_tag="ACHK49_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO9263980.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            9894..10502
                     /gene="yiiX"
                     /locus_tag="ACHK49_003385"
     CDS             9894..10502
                     /gene="yiiX"
                     /locus_tag="ACHK49_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO9263981.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYIFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            10647..11322
                     /locus_tag="ACHK49_003386"
                     /pseudo
     CDS             10647..11322
                     /locus_tag="ACHK49_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         2807 a         3191 c         2879 g         2573 t
ORIGIN      
        1 tctgtgccag taatctgaaa ctcctacgtt gttcaggagt tttttatccg cttctggaca
       61 ttttctctac agaacaatcg ttagcccgga aggtcgaatc tgtgactctt attttcacgg
      121 acgaaccaga tgaaagcttc actggcgctg ctcagcctgc taacggcatt taccagccac
      181 tccttaaaat ctcccgccgt tccaccaacc gtggtacaaa tccaggccaa taccaacctt
      241 gccatcgcag acggtgccag acagcaaatt ggcagcacgc tattttacga tccggcgtat
      301 atgcagctta cctatcctgg cggtgatgtc ccgcaagaac gcggggtatg ttccgatgtg
      361 gtgatccgtg cattgcgcag ccagaaggtc gatttgcaga aactggtgca cgaagatatg
      421 gcgaagaatt ttgccgagta cccgcaaaaa tggcagttaa agcgcccgga cagcaacatc
      481 gatcaccgtc gggtgcctaa tctggaaacc tggtttaccc gccacgataa aacacgcccc
      541 atcagcaaga accccagtga ctatcaagcg ggcgatattg tctcctggcg actggataac
      601 ggactggcac atattggggt ggtgtcagat ggcttcgccc gcgacggtac gccgctggtg
      661 atacataaca ttggcgcagg cgcgcaggaa gaagatgtgc tgtttagctg gcggatggtg
      721 gggcattatc gttattttgt taaataagcg atcgccccac atgaagaaaa aattcagatg
      781 ctttttacac tgacagcttc ttttttctta cggctcacca gcgccagtgc cagcacaatc
      841 agcacaatac cgctaccttc aacggccccc ggattctcac cgagcagcca ccaggaaaag
      901 agtacgccgc aaacgggaac ggccagcgtg cttaagctgg caatactggc aggcaagttt
      961 ttcaatacaa acaaccataa gctccacgcc agcgccgtcg ccagaatcgc actgtaggcc
     1021 agcgcccaga acacggtggg ctgccagtca atttcacgtt gcggcaccag taaagcgacc
     1081 acactcatca ccagcgccgc atagagcatc tgccaggatg ttaacgacaa taaatccacg
     1141 cgcggatgac gggcatacag acgtttagca acaatcgcgc tcgcccccca actgacgccg
     1201 gagaggattg ccagcatggc acttttcatc gaagagaaat cgagttgcca cggctgcaac
     1261 accaaaaata agccgaaagc ggcaatcagt atcgcgaaat attgcccacg tcgcaggcgt
     1321 tcaccgagaa acaacgcggc gaaaatcacc acccagaacg gcatggtata gcttaggatc
     1381 gccaccttcc ccgctccgcc gctgaccagc gcccactgcg ccaggccaac catcccgcag
     1441 gtttgtaaca gggcaatggc taaggtgtat ttaaacggtg tcgggcgcat tccgcgacca
     1501 cgtaataaaa ggacgatgaa taaaacgaga gcgccgaaaa tgcagcgtaa ggcggtaaag
     1561 tcgaaggcac cgatgtagct ggtgacttgc ttcatgaaaa tccagctata actccagata
     1621 agtgtgagca caaccaggcc actgattgcc agtgggttgc tctttcctgc gacagacatg
     1681 agagatccag tgtgtagttt tcctgtggac actatacgcc ggtaaagaaa cttcagcgac
     1741 tgcctgaaag gcagtcgctg aagaaggtca gattacagca ggtcgaaacg gtcgaggttc
     1801 atcactttca cccatgccgc cacgaagtct ttaacaaact tctcgtgagc atcgctgctg
     1861 gcgtaaactt cagccaccgc acgcaggaca gagttagaac caaataccag atccgcacgg
     1921 ctggcagtgt atttcacttc gccggtttcg cggtcacggc cttcaaacag ctctttcgat
     1981 tcgtcggtcg ctttccactc gtaacgcata tccagcaagt tcacgaagaa gtcattgctc
     2041 aatacgccaa cgcggtcagt gaagacgccg tttttgctgc catcgaagtt ggcacccagt
     2101 acacgcatcc cgcccaccag cgcggtcatt tccggcgcgg tcagggtcag ttgctgggct
     2161 ttgtcgatca gcagtgactc ggtggtggaa acatccagac gagcgcgata gttacggaaa
     2221 ccgtcagcaa ttggctccag cagctcgaac atctcaatgt cagtctgatc ctgacgcgca
     2281 tcaacgcggc ccggcgcaaa cggtacatga atgctcaaac ctgcggcgct tgcggctttc
     2341 tcaacaccaa ccacaccagc cagcacgatg atatccgcca gcgaggcttt accagactct
     2401 ttctggattt tctccaaaac aggcagagca cgaacggctg cggcgttcac atcccagtcg
     2461 cgctgcggca ttaatgccag acgcgcaccg ttggcaccac cgcgtttgtc gccaccacgg
     2521 aaggtagaag cagaagccca ggccaccgat accagctcac taacagacag accagaatcc
     2581 gcaatcgcga atttcagatc gataatgtcc tgctcggtcg ggttgtagat cggctgcggc
     2641 agcggatctt gccagatcag atcttctttc ggcacttccg gcccgatgta gcgagatttc
     2701 ggccccatat ccctgtgcgt cagtttgaac caggcacggg caaaggcttc gttgaacgcc
     2761 tgcggatcgt tgaggaaacg acgagagatc ttctcgaact caggatcaaa acgcagcgtc
     2821 aggtcggtca ccagcatcgt cggtttacgt ttcttcgacg gatcaaacgg atccgggata
     2881 atttccggtg cgtctaccgc ttcgaactgg attgcgccag ccgggctgcg ggtctgtacc
     2941 cactcatact tgaacaggtt ctcgaagaaa tagttgctcc actgggtcgg cgtctgggtc
     3001 cagactactt ccagaccaga ggtaatggca tctgcgccaa cgccgctgcc gtaagtgctc
     3061 gcccaaccta aaccttgttc ttcaatcggt gcagcttctg gatcaggacc tacatttgat
     3121 gtcggaccgg caccgtgggt tttacccagc gtatgaccac ccgcaataag cgccacggtt
     3181 tcttcgtcgt tcatgcccat gttgccgaag gtcgcacgga tagctgctgc cgcagaaagc
     3241 ggttcgccgc tgtgatccgg gccttccggg ttaacgtaaa tcaggcccat ctcggttgca
     3301 cccagcggtg ctttcgccag cgcttccgga tgacggtggg tcagccaggc tttttcatca
     3361 ccccagttaa catccagatc cggttcccag acgtcttcac gaccggcacc aaaaccgaag
     3421 gtacggaagc cggagttttc cagcgccacg ttacccgcga ggataaacag gtcggcccag
     3481 gagattttct gaccatattt ctgtttgatt ggccacaaca ggcgacgcgc tttatcgagg
     3541 cttacgttat ccggccagga gttcagcggt gcaaaacgtt gctgaccacg acccgcgccg
     3601 ccgcgtccat cgattgaacg gtaagtcccc gcgccgtgcc aggccatacg aataaacaga
     3661 ccggcgtagc tgccccagtc ggcaggccac cacggctgag attctgtcag cagggctttc
     3721 agatcttttt tcaggccgta gtaatctaat ttgctgaatt ctttgcggta gtcaaagtcc
     3781 tcacccagcg ggttagaacg attagaatgt tggtttaaca ggtcaacacg gagttgattt
     3841 ggccaccagt cgcgagtggt tgtgcccgcc cccgcgctct ggtcgtgacc gccctgatgg
     3901 aacgggcatt tgccagtggc tgtcgtgtta tgaatatcgt ctgacgtgct catcaatgtg
     3961 ctcccctcta cagtgttacc gttacgatac acagcgttag agagaagtta taattgaatt
     4021 aatccacgga tgcgatagtt gagatcttac atattgttgg ttaaagagat gtagatcaaa
     4081 ttgatcgtaa ttaagctttt ttgattatgt ccgaattcgg acaactgctt tataaaaaag
     4141 ggttttagat tcctattctt caaggaagga gggcgaaaac aaagctgaca accttatttt
     4201 tacgccctca tttcgaggca gcattttgtg ctctgtttaa aatttgtgat cacagtgtga
     4261 ttttcacaaa agccacacta tttataaacc aggtcgaaca cccagcgtat ggcaaatcgc
     4321 gtaactcatt tcagcgcggt taagcgtata gaagtggaaa tctttcactc cttcacggct
     4381 taaaatcttc accatatcca tggcaatatt cgcgcccacc agcttgcgcg tttcagcatc
     4441 gtcatccagc ccgtcaaaca tttgtgccat ccacgccgga atacgcacgt tggtcatatc
     4501 ggcaaatttc ttcgcctgtt taaagttaga taccggcaaa atccccggaa taatttccac
     4561 atcaatgccc gccgatacgc agcggtcacg aaaacgcagg tagctttcga catcaaagaa
     4621 gaactgagta atcgcgcggt tggctccggc atcgactttg cgtttcaggt tgagcaaatc
     4681 cgcctgagcg ctttttgctt ccgggtgaac ttccggatac gccgccacgg agatatcgaa
     4741 atctgccact tcttttaaca gcgtcaccag gtcagaagca tacatttccg gcttaccact
     4801 tcccggcggc agatcgccac gcagcgccac gatatgacga ataccgttat tccagtagtc
     4861 gcgtgcaatg gtgcgcagct cgtcgggcgt cgcatcaatg caagtaagat gcggtgccgc
     4921 ttccagacca gtgcgatctt taatgccttt aataatgctg tgcgtacggt cgcgctcgcc
     4981 ggagttcgcg ccataggtca ccgatacaaa cttcggtttc aggctgctaa ggcgatcgat
     5041 ggagttccac agggtctgct ccatttcact ggtacgcggc gggaaaaact cgaacgaaac
     5101 gttaatctgc ccctggactt ctgccaggct ctgattcagg gcatcccgct ggctggcgtg
     5161 aaaaaagctc ataccttacc tcatcaatcg cttgtagtta tatgttgtgt tgtgcacatc
     5221 tatccattta gacgtccaga tgtaaagatg aaggaaaagc cgaagggcgt caaccgaaaa
     5281 atgaccaatg atggtgagga aaaatcaggg aagatgaaaa aacttcagga tggtaagaaa
     5341 aagaaaatcc ggtatgcaaa gcaaaccgga ctgcaaatat aaactaacca tgtacttatt
     5401 gcttcttgcc ggattcgtcg taaacgcttc atccggccta atcctatgat aattcaatat
     5461 atttcattag aaaccataga cccgaaaaca tgagtaccgg gcattattaa atttctgaaa
     5521 ttacaacaac tgtgccagcc ggttgatatc cgactgaatc gccccggcgg tgacgtcgcg
     5581 cccagcgcca ggcccacgga tcaccagcgg attatcgcgg taccagcggc tttcgatggc
     5641 aaagacgtta tcgcatggca gcagtgatgc cagcggatgg tcttcacgca ccgcttccac
     5701 gcccacacgc gctttgccat tggcatcgaa acgcgccacg tagcgcagca ccagccccat
     5761 ttcgcgggcc gcttccagcc gttgcaccat ctgctcgtta agttcatcgc cattttcaaa
     5821 gaaatggtcg atgctgccgc cttcgcaatg agcaggcacc agcgattcca cacgtacctg
     5881 gtccggttca atgttgtaac ccgcttcacg cgccagaatt accagcttgc gcattacgtc
     5941 tttgccggag aggtcgtcac gcggatcagg ctcggttaag ccctgctgcc acgcctgatc
     6001 caccagctcg gtaaacggca cgctaccgtc gaattgcagg aacagccagg agagcgtgcc
     6061 ggagaagatc ccgctgatcg acaaaatagt atcgccgctg tcgatcagat cgcgcacggt
     6121 gtggttgatc ggcaagcccg caccgacggt ggcattgtac agccagtgac gcccggtttt
     6181 ttcgaaggcg tcgtggatct ggcgatattt attgctgtcg ctggctcccg ccagtttgtt
     6241 ggcgctgata acgtggaaac cgtggctggc gaaatcaaga tactgatcag caagctgctg
     6301 gctggcggta acgtccagca ccactaaatc atcatacgga tgggcgcgca tccacaggaa
     6361 caacgactct tcatcctgct caaccgcttc atcgttgaag aaggctaacg cgcggctggc
     6421 gtccagcccg tcatagctca acaggctgcg gcggctgtcc accacacctg ccagcacaaa
     6481 ctcaaagccg gtacgtgccg aaagcgtgct ctgctcacgg gcgaacagtt ccagccaacg
     6541 ggaaccgata ttgcccttac cgaacaatac caggccgatg cgtttttctg cgcggaagac
     6601 ggactgatgc agcccctgaa tcaggctttc ggtcgggccg gtgcgcagta ctgccaccag
     6661 gctgatgccg tcatcggact gccaggtaaa ttcgaccggc tggcctttca gttgctgcca
     6721 gaagcggtgg caatgcagcg ggttacgggt gacgcctgca ccgaccatcg ccaccagcgc
     6781 cagcccctga cgcaggcgca gttcgccagg taatcccgct tcgtcgagga ttttcagcgc
     6841 actgtcggcc acttctgagg tgtagcaaaa ttgcagcaac tggcgatcgt tatgtacgcc
     6901 aaccgccagc gggcgtacct gcgcgcgttt caggatttgg tcgatctctt tatgcgccag
     6961 tttgaaatcc tgactgacgg gcacctgaaa ctcaatcaaa cagacatcat cgtggctggt
     7021 gacaatacgc gcaccagtac cggaggccag cacgcgttca atgcgcgtgg aaccttgatc
     7081 cggcgtgtag ctacagcgca gttgcaggtc gatttcgctg ccagaaaccg gctgtaaagt
     7141 acgggcgtga agaacgggag ccgccaggcg cgccagttcg ctggcctcat ccagacgcag
     7201 caacggcagc aggcaggcat ctttcacttt acgcgggtcg gcactgtata ccccggcgac
     7261 gtcgctccag atggttacgc gagaaacacc cgccagcgca ccgatttgtg tcgcggaata
     7321 gtcggaaccg ttacgcccca gcagcaccgt ttcaccggcg ttgttgcggc tgataaatcc
     7381 ggtcaccacc agacgtttgc ccggatgttg caccagcagc tgttgcagca acgggtaaga
     7441 aagcccttca tcaacctgcg gttgtgcggc gcgttcagcg cgtaaaaact cgcgggcatc
     7501 aagccaggcc gctggcagcc cttgttgatt aagtaccgca gacatcaggc gtgccgacca
     7561 tacttccccg tggcccacca cttccgcata cactgcgtcg ttaataccgc tgtcgagcag
     7621 cgccgccagg cgctcaaggt cgctgacaaa agcgctaatg aggctatcgg cttcttcagc
     7681 gggtagcaga ccgctaatca gatcgcactg atagcgacgc agcgtttgtt gaacctgatg
     7741 cgcagagaga cgatcggtct ggcttagttt caaccagtta atcaactggt tagtggtgct
     7801 accggcggcg gaaaccacca tcatatcgtc aggctgagag tactccgcca taatgcccgc
     7861 gacacgcaaa taacacttca catcagccag actactgcca ccaaatttat gcagctgacg
     7921 acctttcgcc cctgcctgcg caatcacact catttttacc ccttgtttgc agcccggaag
     7981 ccattttcca agtcggcaat taaatcttcg ccatcttcaa taccggtgga gatacgcagc
     8041 agcgtctcgg aaatcccggc ggcagcacgc gcttctggtg ccatgcctgc atgtgtcatg
     8101 gttgcggcgt gagagattaa actttccact ccccctaatg attccgccag cgtaaacaac
     8161 gacagcccgc ccaggaaacg acgcagcgtc tgctcatcgc catccagttc aaaactcaac
     8221 attgcgccaa agcctttttg ctgacgcgcg gcaatttcat gcccctgatt ttccggcagc
     8281 gacgggtgat acagtttttt caccaacggc tgggtttgca gatatttcac aatcgcctgc
     8341 gcgttgcgct gcgccagctc catacgcggc accagcgttc gcaacccgcg tagcagcaga
     8401 tagctgtcaa acgcgccgcc cgtcacgcca atattgtttg cccaccaggc aagttcagtg
     8461 acaacgtccg ggtctttagc aatcaccacg ccagccacca cgtcagagtg accgttcaga
     8521 tatttcgtac atgaatgcaa caccagatcg gcacccaatg ccagcggatt ttgtaatgcc
     8581 gggcttaaga aggtgttatc caccacgctc accgccccga cttcccttgc cagatggcag
     8641 attttcgcaa tatccacgac gcgtaacaat ggattacttg ggctttctac cagtaccagt
     8701 ttgggttttt ctgccagtgc tgcccgtaat gcctgttcat cgccttgatc aacaaacaac
     8761 acgcgatagc aaccgcgttt cgccaggctg tcgaacaggc gatagctacc gccgtagcag
     8821 tcgtgcggcg caaccagcag atcgccaggt ttcaaaaaga cggtcattac caggtgaatc
     8881 gcggacatgc cggtattagt aagtactgca ccagcaccgc cttccagttc tgccagcgca
     8941 cgctgaacca catcgcgcgt tgggttgccg cgacgcgagt aatcatgcgc gcgcggttca
     9001 ttaaatccgg taaagttata tgtgctggaa agatggatcg gtgggacaac gcaaccatac
     9061 tgttcgtcgt catttaaccc gctacgcact gcgatggtgg cctgtttacg cgtcatgtga
     9121 tgaagttccc tgggctttgt cggtgaaatg tcaggcacca gagtaaacat tgtgttaatg
     9181 gacgtcaata catctggaca tctaaacttc tttgcgtata gattgagcaa atcccaaata
     9241 gccgttaaaa ttatatgcat tatcacgccg acaggtgcat tacacgatgt cacggtaacg
     9301 cctgtacggt aaactatgcg ggtttacggt cagtacccac ataaattgtg tggtctggtc
     9361 tcaatttatt gacgaagagg attaagtatc tcatggctga atggagcggc gaatatatca
     9421 gcccatacgc tgagcacggc aagaagagtg aacaagtcaa aaagattacg gtttccattc
     9481 ctcttaaggt gttaaaaatc ctcaccgatg aacgcacgcg tcgtcaggtg aacaacctgc
     9541 gtcacgctac caacagcgag ctgctgtgcg aagcgtttct gcatgccttt accgggcaac
     9601 ctttgccgga tgatgccgat ctgcgtaaag agcgcagcga cgaaatcccg gaagcggcaa
     9661 aagagatcat gcgtgagatg gggattaacc cggagacgtg ggaatactaa agcggaaatg
     9721 agcggcgcgg agtggaatcg cctgatgcgc tacgcttatc aggcctacgt catattgcaa
     9781 tttattgaat ttgcacgaac ttgtaggccg gataaggcgt tcacgccgca tccggcataa
     9841 acaacgagca cgttgtctgc gacccaccgc tttttataca tggacgttta actatgaaaa
     9901 acaggctgct gatcctcagc ctgctggttt ctgtacctgc ctttgcctgg cagccgcaaa
     9961 ctggcgacat catctttcaa atctctcgct catcgcaaag taaagcgatc caactggcaa
    10021 cccattccga ttacagccac accggtatgc tggtgatgcg taacaaaaag ccctacattt
    10081 ttgaagcggt cggcccggtg aaatacaccc cgctcaagca gtggattgcc catggtgaaa
    10141 agggcaaata cgtcgttcgc cgcgttgaag gcggactgag tgttgaacaa cagcaaaaac
    10201 tggcgcaaac ggcaaaacgt tatctcggta aaccgtatga tttcagcttc tcatggagcg
    10261 acgatcgcca gtactgttcg gaagtggtgt ggaaggttta ccagaacgcg ctgggaatgc
    10321 gcgtgggcga acagcagaag ttgaaagagt ttgatctcag caacccgctg gtccaggcga
    10381 agctcaaaga acgctacggt aaaaatattc cgcttgagga aacggtagtt tcaccacaag
    10441 ccgttttcga cgcgccacaa ctcaccacgg ttgccaaaga gtggccgctg ttttcgtggt
    10501 aatggatgat gaaaaaaata attcccgccg tagtctgttt gttgctttgt agttgcacga
    10561 cctttccgca agctgtcgct ccggtaaacg gtgatgcctc caattagttg aattaaggat
    10621 ataatactcg cttttaaggt tgtttcatgg tcacagttaa ccttcattgc ccccgttgcc
    10681 agtccgttca ggtttaccgt cacgggcaga acccaaaaag acatgatcga ttccgcggcc
    10741 gtgactgtca ccgggtattt cagctcagtt actgttacga gccccgtaaa ccgggagtta
    10801 aagaccaaat cgctgaaatg actttcaacg gtgccggggt tcgcgatact gcccgtacgc
    10861 tgaaaatcgg cattaataca gtcatccgaa ctttaaaaaa ctctcgccac gaagaataac
    10921 gtcatccccg gtcgctcatg ctgatgtggc actcatctgc aaacttagtc agcaatggag
    10981 ctttgtcggc agtaaagcgc gacagcactg gctctggtac gtgtacaaca cgaaaactgg
    11041 tggtgttctg gcctacactt ttggcccccg gactgacgaa acctgtcgtg aattgcgggc
    11101 gctgcttacc cttttaccat cggcatgttg accactgacg aatggggtag ttacgctaga
    11161 gagttaccga aggagaagca tctgactggt aagattttta ctcagcacat tgagcgtaac
    11221 aatctgaccc tgagaacccg aatcaaacgt ctggcccgca gaaccatctg tttctcgcgt
    11281 tctgtagaac ttcatgaaaa agtcatcggt gcttttatcg aaaatatatg ttctactaat
    11341 tggagtcatc acccttctga taagctttcc ttggatgatt accagactga atacacagcc
    11401 aaagattttt acctcattct gctctttttt ctcaaatgac atatattata
//
LOCUS       DAZGLJ010000144        11385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000144 DAZGLJ010000000
VERSION     DAZGLJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.144"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(55..1137)
                     /gene="lptG"
                     /locus_tag="ACHK49_003387"
     CDS             complement(55..1137)
                     /gene="lptG"
                     /locus_tag="ACHK49_003387"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO9263982.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(1137..2237)
                     /gene="lptF"
                     /locus_tag="ACHK49_003388"
     CDS             complement(1137..2237)
                     /gene="lptF"
                     /locus_tag="ACHK49_003388"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO9263983.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMCTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(2300..2350)
                     /gene="ytgA"
                     /locus_tag="ACHK49_003389"
     CDS             complement(2300..2350)
                     /gene="ytgA"
                     /locus_tag="ACHK49_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO9263984.1"
                     /translation="MSIIILATISTTQENK"
     gene            2504..4015
                     /gene="pepA"
                     /locus_tag="ACHK49_003390"
     CDS             2504..4015
                     /gene="pepA"
                     /locus_tag="ACHK49_003390"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO9263985.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            4369..4812
                     /gene="holC"
                     /locus_tag="ACHK49_003391"
     CDS             4369..4812
                     /gene="holC"
                     /locus_tag="ACHK49_003391"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO9263986.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTC
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            4812..7667
                     /gene="valS"
                     /locus_tag="ACHK49_003392"
     CDS             4812..7667
                     /gene="valS"
                     /locus_tag="ACHK49_003392"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO9263987.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(7721..8917)
                     /gene="yjgN"
                     /locus_tag="ACHK49_003393"
                     /pseudo
     CDS             complement(7721..8917)
                     /gene="yjgN"
                     /locus_tag="ACHK49_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
     gene            9110..9613
                     /gene="yjgM"
                     /locus_tag="ACHK49_003394"
     CDS             9110..9613
                     /gene="yjgM"
                     /locus_tag="ACHK49_003394"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9263988.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(9659..10075)
                     /gene="rraB"
                     /locus_tag="ACHK49_003395"
     CDS             complement(9659..10075)
                     /gene="rraB"
                     /locus_tag="ACHK49_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO9263989.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            10237..11250
                     /gene="argF"
                     /locus_tag="ACHK49_003396"
     CDS             10237..11250
                     /gene="argF"
                     /locus_tag="ACHK49_003396"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO9263990.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGAHVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         2985 a         2916 c         2887 g         2597 t
ORIGIN      
        1 ctcgtatttg actttatcaa caatgtgaaa gggagctttc gctcccttgc ttggttacga
       61 ttttctcatt aacagccaca ggctgattaa gaagaagctg gcgcttggca acagtgcgcc
      121 gataatcggc gggatgccgt aaaccaacgt cagcgggccg aagatctggt ccagtacgta
      181 gaagacaaaa ccgaagctga taccggtgac cacacgcacg cccatcggta cgctacgcag
      241 tgggccaaag atgaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa
      301 gattttgctc cacatattga gctgataacg tccggcatcc tgaccgctcg acttcagata
      361 cttcacgtag ttgtgcaaac cgctgattga gagtgcatcc gggtccagcg ccaccacgcc
      421 cagtttgtct ggcgtgaggt tggttttcca ggtgccgctc accgtctgcg aaccggtgat
      481 ctgtttcgga ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc
      541 cgggtcaaac ttcgcagtag cggcatagcg tacggattgc agacgacgat tttcgttaaa
      601 ggcataaatg ctgatgccac ctaactcttc gtcaccttta acccgctcaa tgtagacgaa
      661 gttgttgcca tctttcgccc ataagccttg ctgggtagag agcaacgagc cgccgtacat
      721 cgcctgcgca cggtagttac gcgccatctg ctcgccctgc ggcgcgaccc attcgccaat
      781 cgccatcgtc agcaagacca gcggaatggc ggttttcatc accgacagcg ccacctgcat
      841 acgggtaaaa ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag
      901 accaagcaac gccccaagca gagccgccat cgggaagaag atttgcacat ctttcggcac
      961 gctcagcaag gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt
     1021 caactgatcg acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat
     1081 gatggtggtg aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac
     1141 accgctcctt tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg
     1201 ttgagaacaa tcgccaaagc cagataaatc aggttaacgg tccacatcca cagcgtcggg
     1261 tccagcttac ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga
     1321 tacagcagca tggctggcag catcgacagt acgcgtccct gacgtgggtt aaccacgctc
     1381 agcggtacga ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc
     1441 agttctgcgc gagcacgatc ggtgtcagtg ttccacaatg tgcacatgtc catctggtcg
     1501 gtatcgttcg ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa
     1561 tccgtaatgc ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga
     1621 gtgacgacct gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca
     1681 gaaggacgtg cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg
     1741 ccgtcaacgc tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct
     1801 tgcgccagcg ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga
     1861 cgcgatgacc acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc
     1921 gcaaggatca ttgccgcttt caccagaacc gctttgctca ggccgcaggc atgcattacc
     1981 gtaatttcac tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt
     2041 aatggcagga taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga
     2101 ttcgccggaa tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag
     2161 aagatcaaaa gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga
     2221 tatcttatga ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc
     2281 ttgtttcatg gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa
     2341 ttatactcaa cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt
     2401 taagattaac acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat
     2461 ctgtagccac cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa
     2521 aagcggtagc ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg
     2581 tcgcctttct ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct
     2641 gctacgtcgg ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc
     2701 gaatgtactt tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga
     2761 gcgtcagtac aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcaat
     2821 ggaagcggtc tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt
     2881 gcgtcaggct gtcgagacgg caaaagaaac gctctacagt ttcgatcagc tgaaaacgaa
     2941 caagagcgaa ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga
     3001 actgaccagc ggtgagcgcg cgatccagca cggtctggcg attgccgccg ggattaaagc
     3061 agcaaaggat cttggcaata tgccgccgaa tatctgtaac gccgcttacc tcgcttcaca
     3121 agcgcgccag ctggctgaca gctacagcaa gaatgttatc acccgcgtta tcggcgaaca
     3181 gcagatgaaa gagctgggga tgcattccta tctggcggtc ggtcagggtt cgcagaacga
     3241 atcgctgatg tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt
     3301 gctggtgggt aaaggtttaa ccttcgactc cggcggtatc tcgatcaagc cttcagaagg
     3361 catggatgag atgaagtacg atatgtgcgg tgcggcagcg gtttacggtg tgatgcgtat
     3421 ggtcgcggag ctacaactgc cgattaacgt tatcggcgtg ttggcaggct gcgaaaacat
     3481 gcctggcgga cgagcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt
     3541 tgaagtgctg aatactgacg ctgaaggccg cctggtactg tgcgacgtgt taacttacgt
     3601 tgagcgtttt gagccggaag cggtgattga cgtggcgacg ctgaccggtg cctgcgtgat
     3661 cgcgctgggt catcacatta ccggtctgat ggcgaaccat aatccgctgg cccatgaact
     3721 gattgccgcg tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta
     3781 tcaggaacag ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg
     3841 ggcgattacc gcaggttgct tcctgtcgcg ctttacccgt aagtacaact gggcgcacct
     3901 ggatatcgcc ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt
     3961 agcgttgctg gcacagttcc tgttgaaccg cgctgggttt aacggcgaag agtaattgcg
     4021 tcaggcaagg ctgttattgc cggatgcggc gtgaacgcct tatccgacct acacagcact
     4081 gaactcgtag gcctgataag acgcgcaagc gtcgcatcag gcaatgctgt tattgtcgga
     4141 tgcggcatga acgccttatc cgacctacac agcaccgaac tcgtaggcct gataagacac
     4201 aacagcgtcg catcaggcaa tgctgttatt gtcggatgcg gcgtgagcgc cttatccgac
     4261 ctacacacag cactgaactc gtaggcctga taagacacaa cagcgtcgca tcaggcgctg
     4321 cggtgtatac ctgatgcgta tttaaatcca ccacaagaag ccccatttat gaaaaacgcg
     4381 acgttctacc ttctggacaa tgacaccacc gtcgatggct taagcgccgt tgagcaactg
     4441 gtgtgtgaaa ttgccgcaga acgttggcgc agcggtaagc gcgtgctcat cgcctgtgaa
     4501 gatgaaaagc aggcttaccg gctggatgaa gccctgtggg cgcgtccggc agaaagcttt
     4561 gttccgcata atttagcggg agaaggaccg cgtggcggtg cgccggtgga gatcgcctgg
     4621 ccgcaaaaac gcagcagcag cccgcgcgat atattgatta gtctgcgaac atgctttgca
     4681 gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga ttctctgaaa
     4741 caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct gaatacggca
     4801 acctggaaat aatggaaaag acatataacc cacaagatat cgaacagccg ctttacgagc
     4861 actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa agtttctgca
     4921 tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc ttccagcaaa
     4981 ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc ctgtggcagg
     5041 tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag attgccgcag
     5101 aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa atctgggaat
     5161 ggaaagcgga atccggcggc acgattaccc gtcagatgcg ccgtctcggc aactccgtcg
     5221 actgggagcg tgaacgcttc acgatggacg aaggcctgtc caatgcggtg aaagaagttt
     5281 tcgttcgtct gtataaagaa gacctgattt accgtggcaa acgcctggta aactgggacc
     5341 cgaaactgcg caccgctatc tctgacctgg aagtggaaaa ccgcgaatcg aaaggttcga
     5401 tgtggcacat ccgctatccg ctggctgacg gtgcgaaaac cgcagacggt aaagattacc
     5461 tggtggttgc gactacccgt ccggaaaccc tgctgggcga taccggtgta gccgttaacc
     5521 cggaagatcc gcgttacaaa gacctgattg gcaaatatgt cattctaccg ctggttaacc
     5581 gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc ggctgcgtga
     5641 agatcacccc ggcgcacgac tttaacgact atgaagtcgg taaacgtcac gccctgccga
     5701 tgatcaacat cctgaccttt gacggcgata tccgtgaaag cgcccaggtg ttcgatacca
     5761 aaggtaacga atctgacgtt tattccagcg agatcccggc agaattccag aaactggagc
     5821 gttttgccgc acgtaaagca gtcgttgctg cggttgacgc actcggcctg ctggaagaaa
     5881 ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtagtt atcgaaccaa
     5941 tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggcg gttgaagcgg
     6001 ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtat ttctcctgga
     6061 tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac cgtatcccgg
     6121 catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa gtgcgcaagg
     6181 aaaataacct cggtgccgac gttgccctgc gtcaggacga agacgttctc gacacctggt
     6241 tctcttctgc gctgtggacc ttctctaccc ttggctggcc ggaaaacacc gacgccctgc
     6301 gtcagttcca cccaaccagc gtgatggtat ccggcttcga catcatcttc ttctggattg
     6361 cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa ccgcaggtgc
     6421 cgttccacac tgtttacatg accggtctga ttcgtgacga cgaaggccag aaaatgtcca
     6481 aatccaaggg taacgttatc gacccgctgg atatggttga cggtatttcg ctgccagaac
     6541 tgctggaaaa acgtaccggc aatatgatgc agccgcagct ggcggacaaa atccgtaagc
     6601 gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc ctgcgcttca
     6661 ccctggcggc gctggcgtct accggtcgtg acatcaactg ggatatgaag cgcctggaag
     6721 gttaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg atgaacacag
     6781 aaggtcagga ttgcggcttt aacggcggcg aaatgacact gtcgctggcg gaccgctgga
     6841 ttctggcaga gttcaaccag accatcaaag cgtaccgcga agcgctggac agcttccgct
     6901 tcgatatcgc tgcaggcatt ctgtatgagt tcacctggaa ccagttctgt gactggtatc
     6961 tggagctgac caagccggta atgaacggtg gcaccgaagc cgaactgcgc ggtactcgcc
     7021 atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc attccgttca
     7081 tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc gacaccatca
     7141 tgctgcagcc gttcccgcag tacgatgcat ctcaggttga tgaagccgca ctggccgaca
     7201 ccgaatggct gaaacaggcg atcgttgcgg tacgtaacat ccgtgcagaa atgaacatcg
     7261 cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca gaacgtcgcg
     7321 taaatgaaaa ccgtggcttc ctgcaaaccc tggcgcgtct ggaaagcatc accgtgctgc
     7381 ctgccgatga caaaggtccg gtttccgtta cgaagatcat cgacggcgca gagctgctga
     7441 tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgtctggcg aaagaagtgg
     7501 cgaagatcga aggcgaaatc agccgtatcg agaacaagct ggcgaatgaa ggctttgtcg
     7561 cccgcgcacc ggaagcggtc atcgcgaaag agcgtgagaa gctggaaggc tatgcggaag
     7621 cgaaagcgaa gctgattgaa cagcaggctg ttatcgccgc gctgtaatca acaccgtgat
     7681 gaaaacgaag gccggagaat gctccggcct tttttatctc ttacagaaat ggtaaagaag
     7741 gcataattcc ccgtgaaata ctggctaaga agcctttatc tggttgttct tctttattga
     7801 ttaaaggaag gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc cagtcaatca
     7861 tccatatttt cagcagtgga taagccagac cgcccgtaat cccggatatc actaccaacg
     7921 cacacatgcg ataaagcata ccgtggtaag ttaaagttga gcgaaaacga atacgcccat
     7981 cattcagtga caggttgctc ataaaatgat ttcgcaacga caccgttaaa taactggtgc
     8041 taacagcaat cccaaaataa tagattaact gcgcgattat cattttacgt tgcatttcca
     8101 taaattgctg taaattctca atatcatcat ttgcatacac agaactatca tacgcattta
     8161 atatttgatc gaagataata taaccagcaa cagcgagaaa aggtaataaa gctaaaaatg
     8221 cgaggatggc atattttata caatacgcag tatctaattt caccttgaaa cgatgtatac
     8281 cgaaactggt attgctccag agaaaactca ttaccaggct atataaagta ccattaaaaa
     8341 taccaatgga aacaatacca actattgcca tcagaaccac agatataata acactacttg
     8401 aactattggc atgtagcatc tttgtcgaga taaagaaaac agtccccatc ccaatggcca
     8461 ttaaaatcgg caagaaaaag gttactcacc agaacccttt catagagcaa ttaaaactaa
     8521 atcttacgcc gttgagacta gtcatcaagg cctgataacg cagtcctttt gctgccataa
     8581 atataagcaa aaccaacagt gacaaagtca aaacacagcc aataagcggc atatctgctg
     8641 acactgtcat taagattgcg aaatagaaaa aaacaaaaac aagacaacta acaaaaacat
     8701 tcccaccggt aatcccataa gaaaatcgtt gtccgttaac ctccatatta gcatagagat
     8761 aacgcttaca tttcattaat gcccatggta aatagatacc tagcgttata atcgttaaca
     8821 acacattcac cacacaaata agaaaatatc tctcacctgt accatgaaaa acaaacgaat
     8881 gggacggaac atccatttca ttaataactt gagccataaa aattccctga attaaaaaat
     8941 ccattgatat aagttataga tacagacagt aaaatgttct gccacgttac aatactctta
     9001 tcagtaatag gctggcaaaa actttattgc tttataagat gcttgcgcac gtaagaagag
     9061 agtggtatta aacaaatgta taattacttt atttttattg ggttaaacta tgaataacat
     9121 tgcgccgcaa tcacctgtaa tgcgtcgcct gacgctgcag gataatcctg ctattgcccg
     9181 cgtcattcgt caggtatccg ccgaatacgg tcttaccgct gataaaggct acaccgtcgc
     9241 cgatccgaat cttgacgagc tgtatcaagt gtatagtcag ccgggtcatg catattgggt
     9301 ggttgagtac gagggtgaag tggtcggcgg cggtgggatt gcgccattag caggtagtga
     9361 atcggacatt tgcgaactgc aaaagatgta ttttcttccg gctattcgcg gtaaagggct
     9421 ggcgaaaaaa ctggccttaa tggcgatgga gcaggcacga gagatgggct tcaaacgctg
     9481 ctatctggaa acgaccgctt ttttaaagga agccattgcg ctttatgagc atttgggctt
     9541 tgagcatatc gactatgcgc ttggctgcac gggccatgtc gattgtgaag tgcggatgct
     9601 acgcgaactc taaaaaaatg cccgctagtg tgtgctatcg ggcattttaa tcaattaatt
     9661 agtggcgaac cccgtcatcg tcttcatcga caaaatcttc atcgtcgccg tcttcgccgt
     9721 tgggatcttc aaagtaagtg ccccaaccgt cgtactcaac gtcaaatttc tccgccagcg
     9781 tcatcagttg ttcaacctgg gcatcgatca gatcggcatt caacgcgcac tcgctgagga
     9841 tgtcgcagca aatcacgata tcaccatctt caacttccag ctcttctgga tcggtcactt
     9901 cgtaaccgag tttaaacgct tcaactgctg ctttttccag ggtttccaga tcgtctgcgg
     9961 aaagatggtg ttcgatggta tacagcgcgt ccggatcgct gccatcttcc agtaattctt
    10021 caataatcaa acgtgtttct tcacgctgtt cttccagttg ttccgggttt gccatggctc
    10081 attcctctta atgtcctgcc gatactctta ttgtcacaca ccactgacat tgcctccacc
    10141 tttgtgacaa agatttatgc tttagacttg caaatgaata atcatccata taaattgaat
    10201 tttaattcat tgaggtgtta gccacaggag ggatctatgt ccgggtttta tcataagcat
    10261 ttcctgaaat tactcgattt cacgccagct gaactcaaca gcctgctgca gttagccgcg
    10321 aagctgaaag ccgataagaa aagcggcaaa gaagaagcca aactcactgg taaaaacatc
    10381 gcgctcatct tcgaaaaaga ctcgacccgt acccgatgct ctttcgaagt tgccgcatat
    10441 gaccagggtg ctcacgttac ttatctcggc ccaagcggca gtcagattgg tcataaagag
    10501 tcgattaaag acactgcccg cgtgctgggt cgcatgtatg acggtattca gtatcgcggc
    10561 tatggtcagg agattgtcga aacactggcg gaatacgctg gcgtgccggt atggaacggc
    10621 ctgaccaatg agtttcaccc cacgcagttg ctggcggatc tcctcaccat gcaggagcat
    10681 ttacccggta aagcattcaa cgaaatgacg ctggtctatg caggtgacgc acgtaacaac
    10741 atgggcaatt cgatgctcga agctgcggcg cttaccggtc tggatttgcg tctggtcgcg
    10801 ccacaggcat gctggccgga agctgcgctg gttacggaat gccgcgctct ggcacagcaa
    10861 aatggcggga atattacgct gacggaagat gtagctaaag gagttgaagg tgctgacttt
    10921 atctataccg atgtatgggt gtcgatgggt gaagcaaaag agaaatgggc tgaacgcatt
    10981 gcattgctgc gtgattatca agtgaacagc aagatgatgc agttgaccgg taacccggag
    11041 gtcaaattcc tccactgcct gcccgcgttt catgacgacc aaacgacgct tggcaagaaa
    11101 atggcggaag agtttggcct acatggcgga atggaagtga ctgatgaggt cttcgaatct
    11161 gccgccagca ttgtatttga tcaggcggaa aaccgcatgc atactatcaa agcggtgatg
    11221 gtcgcgacgc tcagtaaatt aaataattaa tatctctatc aagatgccgg gaaatacaaa
    11281 aacatcccgg catcacatta ttttatgcag tgatagtttt tttcttccag ccaaattttt
    11341 ctattgctat acgtaaattt tccggaggat ttaattttcc caact
//
LOCUS       DAZGLJ010000145        11370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000145 DAZGLJ010000000
VERSION     DAZGLJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.145"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..389
                     /locus_tag="ACHK49_003397"
     CDS             <1..389
                     /locus_tag="ACHK49_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO9263991.1"
                     /translation="QRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQRRELVALR
                     SNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAAELAADCHV
                     IPRAGHNAHRENPAGVIASLAQILRF"
     gene            404..1261
                     /gene="menB"
                     /locus_tag="ACHK49_003398"
     CDS             404..1261
                     /gene="menB"
                     /locus_tag="ACHK49_003398"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO9263992.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            1261..2223
                     /gene="menC"
                     /locus_tag="ACHK49_003399"
     CDS             1261..2223
                     /gene="menC"
                     /locus_tag="ACHK49_003399"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO9263993.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            2220..3575
                     /gene="menE"
                     /locus_tag="ACHK49_003400"
     CDS             2220..3575
                     /gene="menE"
                     /locus_tag="ACHK49_003400"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO9263994.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            3685..3951
                     /gene="pmrD"
                     /locus_tag="ACHK49_003401"
     CDS             3685..3951
                     /gene="pmrD"
                     /locus_tag="ACHK49_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO9263995.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(3945..4331)
                     /gene="arnF"
                     /locus_tag="ACHK49_003402"
     CDS             complement(3945..4331)
                     /gene="arnF"
                     /locus_tag="ACHK49_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO9263996.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(4331..4666)
                     /gene="arnE"
                     /locus_tag="ACHK49_003403"
     CDS             complement(4331..4666)
                     /gene="arnE"
                     /locus_tag="ACHK49_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO9263997.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4663..6315)
                     /gene="arnT"
                     /locus_tag="ACHK49_003404"
     CDS             complement(4663..6315)
                     /gene="arnT"
                     /locus_tag="ACHK49_003404"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO9263998.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(6315..7205)
                     /gene="arnD"
                     /locus_tag="ACHK49_003405"
     CDS             complement(6315..7205)
                     /gene="arnD"
                     /locus_tag="ACHK49_003405"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO9263999.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(7202..9184)
                     /gene="arnA"
                     /locus_tag="ACHK49_003406"
     CDS             complement(7202..9184)
                     /gene="arnA"
                     /locus_tag="ACHK49_003406"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO9264000.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(9184..10152)
                     /gene="arnC"
                     /locus_tag="ACHK49_003407"
     CDS             complement(9184..10152)
                     /gene="arnC"
                     /locus_tag="ACHK49_003407"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO9264001.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(10156..11295)
                     /gene="arnB"
                     /locus_tag="ACHK49_003408"
     CDS             complement(10156..11295)
                     /gene="arnB"
                     /locus_tag="ACHK49_003408"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO9264002.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTPMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTTIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
BASE COUNT         2768 a         2950 c         3043 g         2609 t
ORIGIN      
        1 gtcagcgttc cgatcgccaa tgggcgcagc gtttttgcac agaaccgtta acggcggtat
       61 ttgccgactg gtatcaacag cctgtttttg cctcgctcaa tgacgatcaa cgccgggagc
      121 tggtggcgct gcgcagcaac aataatggcg caacccttgc cgccatgctg gaggcgactt
      181 ctctcgccgt ccagcctgat ttacgtgcta accttagcgc ccgcacattt gcgttttatt
      241 atttatgtgg tgaacgtgac agcaaattcc gcgccctggc ggcggaactg gctgccgact
      301 gccatgtcat tcctcgcgcc ggacataacg cgcatcggga aaatcccgct ggcgtaatcg
      361 caagtctggc gcagatcttg cgtttctgac taaaggacac aatatgattt atcctgatga
      421 agcaatgctt tacgcaccgg ttgaatggca cgactgctcc gaaggtttcg aggacattcg
      481 ttatgaaaaa tccaccgacg gtatcgcaaa aatcaccatt aatcgtccgc aggtgcgcaa
      541 tgccttccgt cctctgacgg taaaagagat gatccaggca ctggcagatg cgcgttatga
      601 cgacaatatc ggcgtgatca ttctgactgg cgcaggcgat aaagcgttct gctccggtgg
      661 tgaccagaaa gtgcgtggtg attacggcgg ctataaagat gattccggcg tacatcactt
      721 gaacgtgctg gacttccagc gtcagatccg tacctgtccg aaaccggttg tcgcgatggt
      781 ggctggctac tccatcggcg gtggtcacgt tctgcacatg atgtgcgacc tgactatcgc
      841 agcagataat gccatcttcg gtcagactgg cccgaaagtc ggttccttcg acggcggctg
      901 gggcgcttcc tacatggctc gtatcgtcgg gcagaaaaaa gcgcgagaaa tctggttcct
      961 gtgccgtcag tacgacgcaa aacaggcgct ggatatgggc cttgtcaaca ccgtggtacc
     1021 gctggcggat ctggaaaaag aaaccgtccg ttggtgccgc gaaatgctgc aaaacagccc
     1081 gatggcgctg cgctgcctga aagctgcgct gaacgccgac tgtgacgggc aggcggggct
     1141 gcaggagctg gcgggcaacg ccaccatgct gttctacatg acggaagaag gtcaggaagg
     1201 tcgcaacgct ttcaaccaga aacgtcagcc tgatttcagc aaatttaaac ggaatccgta
     1261 atgcgtagcg cgcaggtata ccgctggcag atccccatgg acgcgggggt ggttctgcgc
     1321 gacaggcggt taaaaacccg cgacgggctg tacgtttgcc tgcgtgaggg cgagcgcgaa
     1381 gggtgggggg agatctcccc actgccgggc ttcagccagg aaacctggga agaggcgcaa
     1441 agcgtgctgc ttgcctgggt aaataactgg ctggcaggcg attgcgagct accgcagatg
     1501 ccttccgttg cctttggcgt aagctgtgca ttggcggagc tggcagatac gctcccgcag
     1561 gcggccaatt accgtgcggc accactgtgt aatggcgatc ctgacgatct gatcctcaaa
     1621 cttgctgata tgccaggcga gaaagtggcg aaggtcaaag tgggattgta cgaagccgtg
     1681 cgcgacggca tggtggttaa tctgttgctg gaggcaattc cggatctgca tttgcgtctt
     1741 gacgcaaatc gcgcctggac accgctgaaa ggccagcagt ttgccaaata tgttaacccg
     1801 gattatcgcc accgcatcgc gtttctcgaa gagccgtgca aaacccgcga tgattcgcga
     1861 gcgtttgccc gtgaaaccgg cattgccatt gcctgggatg aaagcttgcg cgagccggat
     1921 tttgcctttg tggctgaaga gggcgtgcgc gcggtagtta tcaaacccac gctcacgggc
     1981 agtctggaga aagtacgcga gcaggtacag gcggcgcacg cgctggggct gacggcggtg
     2041 atcagttctt ccattgaatc gagcttaggc ttaacgcaac tggcgcggat tgccgcctgg
     2101 ttaacgccgg acaccattcc agggctggac acgctggatc tgatgcaggc gcagcaggta
     2161 cgtcgctggc cgggtagcac gctgcctgtc gtggaagttg atgcactgga gcggttgtta
     2221 tgatcttctc tgactggccg tggcgtcact ggcggcaagt gcggggagaa gccatcgcct
     2281 tacgtcttaa tgacgagcaa cttaactggc gcgagctttg tgctcgcgtc gatgaattag
     2341 cctcaggctt tgcggcgcag ggcgttgttg agggcagcgg cgtgatgttg cgggcgtgga
     2401 atacgccgca aacgctgctc gcctggctgg cgttactgca atgcggggcg cgggtgttgc
     2461 ccgtgaaccc tcagctgccg caaccgttgc ttgaagaatt gctgcccaat ctgacgctgc
     2521 aatttgcgct ggtgccggat ggggaaaaca cgtttccggc gttaacgtcg ctgcacattc
     2581 agcgggttga aggcgcacat gccgctacgt ggcagccgac gcgtctgtgc tcaatgacgt
     2641 tgacctcggg ttctaccggg ttgccgaaag ccgctgtcca tacttatcag gcccatcttg
     2701 ccagtgccga aggggtgtta tcgttgattc cgtttggcga tcacgatgac tggttgctct
     2761 ctttaccgct gtttcacgtc tccggtcagg gaattatgtg gcgctggtta tacgctggtg
     2821 cgcggatgac ggtgcgtgat aaacagccat tggagcaaat gctggcaggc tgtactcatg
     2881 cttcactggt gccaacacaa ctctggcgtt tgctggttaa ccgcagttcc gtttccctga
     2941 aagcggtgtt acttggcggc gcggctatcc cggtcgagtt gacggaacag gcgcgcgagc
     3001 aggggattcg ttgcttttgc ggctatggtc tgaccgagtt tgcctccacg gtgtgtgcga
     3061 aagaagccga cggcctggca gacgttggtt cgccgctgcc gggtcgggaa gtgaaaatcg
     3121 ttaataatga agtgtggctg cgggctgcca gtatggcaga aggttactgg cgtaacgggc
     3181 aactggtttc actggttaat gacgaaggct ggtacgctac gcgcgatcgc ggtgagatgc
     3241 ataatggcaa gctgaccatt gtcggacgtt tagacaatct attcttcagt ggcggagagg
     3301 gtattcagcc ggaagaagtc gagcgtgtta ttgctgcaca tcctgcggtt ttgcaggtgt
     3361 ttatcgttcc cgttgccgac aaggagtttg gtcatcggcc ggtagcggtg gtggagtatg
     3421 accagcaaac cgttgatctt ggcgaatggg tgaaagataa gctggctcgt tttcaacaac
     3481 cggtgcgctg gctgacttta ccgccggagc tgaaaaacgg cggtattaaa atttcacgtc
     3541 aggcgctaaa agagtgggtg caacgtcagc aataattaac cctccgtttc acttctcatt
     3601 tctgccattc cattgccgtt gataaagagt ttatctgtta atctgtaaat tgatgtgaaa
     3661 actcttagca aacaggataa tgcaatggaa tggctggtca aaaaatcgtg ctgcaataaa
     3721 caagataaca gacatgtact tatgctctgc gatgctggcg gcgcgataaa aatgatcgcc
     3781 gaagtgaaga gtgacttcgc cgtgaaagtg ggggatttac tctcgccttt gcagaatgcg
     3841 ctttattgta ttaatcgtga aaagctgcat acggtgaaag tcctctcggc aagcagttat
     3901 agccccgatg agtgggaacg gcagtgtaca gcggcaggga aaactcagta acgttgtttt
     3961 gtcgtgggca ggaaaatcag catcaacccg ctcataatac aggctactcc cagtagtgct
     4021 ttcagcgaaa aagtcccttc ccagccggga agtaccattg aagcaatcca taccagcaca
     4081 taactcatac tgagcaaggc ataggctttg cttagcgcaa gtttatgtag cgttttatac
     4141 caacaaaaaa cggaaagcag ataacccagc agcccgagta acagtatccg ggcatccagg
     4201 ccaaaagcta acagagccgc gataaaatcc cataagtgcg tcatcggcgg cagatgactt
     4261 gccgcaaaac ccaggcttaa ttgcgcaact gaggcaataa tgacgctgaa taatccccat
     4321 atcaggccca ttacaccgta ctcccgagga tcacaatgcc accaataatg aacgccaccc
     4381 cacaccagtg acgcggcgat accggttcgt gccacagttt tactgcagcc agcgtcaccc
     4441 agacaaaatt cagacttaac atcgggtagg caatgcctac cggtacgttc tgcaagacca
     4501 gcagccagag caccatggca agaccaagac aagccagcgc cagtcccagc cacagcacga
     4561 tatgtttgcg ccgtttgttt atcgccacaa agcaggttgc ctgtttctga cacaactgcc
     4621 cggcaacgct aagcaagctg gcaaagacta atgttagcca gatcatttgg gacgatactg
     4681 aatcagcacc agacgctcct gacgatcgat ggcatctgcg ggcggaatgg cgagactgtt
     4741 gatatcttca tcgcggtcaa ccgaaagcac gagagtaata atcccctcct gacgatgttg
     4801 attaagccag tttgcgaact catcaccgct gacaaatctc cctttcgcat ccggataatt
     4861 aaggccgtat ttcaactcac ctgtctggcg atacatgatg atgtcatcgc gttgcaggct
     4921 ccatgccaga cctgcggcaa caccgacgct atcagtaaga atatagcggc ttggctgaag
     4981 tgattcttgt gtcatctcga caaaaaattg cggatgtttt ccttccataa ctctgtcagg
     5041 aattgaaaat cctaccagca acgccagccc cagcgggcaa agcgcggcaa aaggccaggt
     5101 cttttcgacg tttgttaagg tgtaccagcc gaaaaatgcc catagcgaaa aaatcgacca
     5161 ggcacaaaag actttatagc tctcgaaggt ttgccacacc ggcgtgttca ttggtcccca
     5221 cggggagacc acaaatgtgg caataatgcc agtgacgcca aaagcgatgt taatccagcc
     5281 attaatccgc agcgccagag gattattttt tgctgccagc aaagcgtaat gcgccatcag
     5341 cattgccaga gatgcaaagc aggaaagaat ataggtgggc aatttacctt tagcgacgga
     5401 gaaaaacagc agcggcatta tcgtccagct caacaaatag acggttgcgg aatgcttgcg
     5461 gtttttccag cctgtgtaca gtgcaccggg gagtaatccc agccacggca ggctaccggc
     5521 aatgatgacc ggcacgtagt accagaacgg agctctatgt tgggcatcat ccagtgcaaa
     5581 gcgttgaata tgctcaaccc agaaaaaata gtgccagaag tcaggctccc gctgcgctat
     5641 cgccagtccc caagggagaa ccgtcagtac gcaactgata accgccagcc agccgtaaat
     5701 aaagagatct ttccagcgtt tttgcgtcgc tacccatggc agcacgctta ataccggcac
     5761 ggcaagggcg agaaaacctt tggtcatcac ccccatgccg caggtgattc ccagcagtaa
     5821 aaatcctgcg cttttgcctt tccacgtctg tgcctgcatt gccagccaga agctgcacat
     5881 tcccgccacc agccagaatg caataaacgg atcgagcacg gcataagtgc cgatggcata
     5941 gacaataaac aatgagagat aaattactgt ggcgagtaga gccagacgtt tatcgcgcca
     6001 taagcgcaga gtaaaccagg tcaccagcgc ggcagttaac agggtcgcaa agataacgcc
     6061 tgcccgcaca ccaaagttat tcgcgccaaa tagccattgc ccaatgctgt taatccagta
     6121 tccggcaatg ggtttttcga aataacgtag ccctaacaga tggggcacaa tccagtcgcc
     6181 ggatgccagc atttctcgac tgatttccgc ataacgcgtt tcatcgggtt gccagagcag
     6241 acgcgtgctg atcggtaaca ggtaatagca ggcaataaac gcgaagaggc cgataaggta
     6301 acgtaccgat ttcatcagcg actacccgca atttgttggc aacccagcca gccttcacgt
     6361 ccggcaatat ttccgcgaac aacttgtccg agcggcaacg tctctgataa cagttcgctt
     6421 aaagggcaaa atgtcactcc ttcctgagct gcgcgtttga ggagatccac aaaattatgc
     6481 tgataagcgc agccttcaac ttctgcatga atggtataaa ccggcgtgcc tttatctcgc
     6541 aggatgcggt tgagtaacca accgttaaaa tcttctgctt tcacatcccg accaatcact
     6601 tcatcccagg tgggtaaggt caccggaatt tgcgcagtgc cagggtttcc tgattcgagc
     6661 aatggacgga acggcatggc cccacgacaa tcgctgttgt agcgcaaatg gaacgcttct
     6721 tttgcttcga tcaccttctg gtcggcacgc cagcccgcag cggcagaaca ggttaccggt
     6781 tgaccgataa tctcttccag agtgcgaaga ccgcgtgcaa tatcgtcgat cattgtttgc
     6841 cgatcccagt taccgctacg ggcttgccag gcatggtgat cccaggcgtg caggccgact
     6901 tcgtgatgtt tagccgcttc acgaatgata tcggcattgg catgaccaat ctctttacct
     6961 ggccaggccg tacctgccag taaaatatcc cagccataaa gcgatgccgc atttgagcgc
     7021 agcatcttcc acaaaaactg tggcttcacc agtcgccaga gatggcggcc catattgtcc
     7081 gggccgacgc tgaaaaaaat gctggcctga atattatgct tactcaagat ttccagcaga
     7141 cgcggcacgc cttcacgggt gccacgaaag gtatcgacat caatgcgtaa gcctactttg
     7201 gtcatgatgg tttatccgta agatcaacgg tgcgcaggaa gaaatccagc gtttcgtcga
     7261 tggtttcctg catatcaatt ttcggctccc agtccaggca gcggtgggca ttgcggatgc
     7321 tcggtttacg atgctctacg tcctgatatc ctttgccgta gtagctgcta ctttcgacaa
     7381 cgcgaaagcc cgcaaacggt gggaaatgat ggcgcagcgg atgtttttcg aagctcgcca
     7441 gcagcatctc gcccagttcc tcaatgctcg cttcgttctc aggattgcca atgttgataa
     7501 tttcaccgtc gcagcgattt cccgcatttt cgataatgcg gtataacgcc tcgataccat
     7561 cgcgaatatc agtaaagcag cgtttttgtt ttccgccatc aatcagctta atcggtgaac
     7621 cttctaccag attgagaatg agttgcgtaa tagcgcggga gctgccgatt cgtgccgcat
     7681 taaggttatc cagtcgcggt cccatccagt taaacgggcg gaagagggtg aactgtaaac
     7741 cctctttttc gccataggcc cagatcaccc gatcaagtaa ttgttttgat accgaataaa
     7801 tccagcgtgg tttattcacc gggccgacga ttaaattaga atggtcctca tcgaagtatt
     7861 tatcgctaca catcccataa acttctgaag ttgacgggaa gatgattcgc ttacggtact
     7921 tcacgcagta gcggataatg cgcagattct cttcaaaatc gagttcaaat acgcgcagcg
     7981 ggttgcgggt atattcaatc ggcgtggcta tcgccaccag cggcaagacg acatcacatt
     8041 ttttgacgtg atactcaatc cattcggaat gaatactgat atcgccttca acaaagtgaa
     8101 aatgcggatg attcagaaaa cggcttatcg catcactgcc aatatccaga ccgtaaactt
     8161 cataatgatc ttcgcgcagc aggcgttctg tcagatggtt gccaataaag ccattcaccc
     8221 cgaggatgag tacccgggta cggcgtcggg cggtgcaggc aggctggcta ttcaagcgtg
     8281 aaccttgcac caggcccagc gtctgcgcta attgcgagcc ctgcatagta atgccgtcgc
     8341 ccgcctgtcc ggtgacgatt tccagcgcgc catcgccaca ggcaatcagc agtggcgcaa
     8401 cagaaatcac gctccccggc tgtgctttgc tggcatgagg atgaacacgc gacgaccaga
     8461 cggtgaattt ctgattgcca acatagctga aggcacccgg ccacggatcg gcaacggcac
     8521 gtaccatgtt gtgcagtacg gatgccggtt tatgccattc aaggaaactg tcatccggcg
     8581 ttctgcgacc aaaacaggtg gcttcgtttt cgcgctgggc gatttccaga atattaccgt
     8641 gtttaatggc gggtaatgtc tgttccagta gctggcgcgc ggcatggcac aatttatgat
     8701 gcagcgtaat agcgatatcg tctggcgcaa tggcaatgcg cagttgcgcc acaatggccc
     8761 cggcatcggc acgtttcacc attcggtgca atgtaacgcc agtttccgtt tcaccgttga
     8821 ccagcaccca gttcagcggc gcgcgaccac gatattttgg taacagcgaa ccatgcagat
     8881 taaatgcacc tgcgggagcg agctgcaaaa tttcgtcgca aataagatgg cgataataaa
     8941 aagagaaaat cacatctggc gacagttggg caatgcgttc cacccacagc ggatgattaa
     9001 cgtcatccgg cgcataaacc ggaatgcctc tttccgctgc cagacgagcc accgaaccat
     9061 aaaaggcttt ttcaccggga ttatcggtat gggtaaaaat ggcgctaatt tcgtaaccgg
     9121 cagccagcag agcttcaata ccgaggcatc ccatatcgtg gtaggcaaaa acgacggttt
     9181 tcattattca ttttccttgc tggatggacg gataacttgc tgaacaaaat agcgggggcg
     9241 ggcgcggaca tcggtgtaga tcctgccgat atattcaccg agtaatccca tgccgataaa
     9301 ctgagcgcca ataaaagtaa acagcacggc aaatagcata aagacgcctt ctgccgccca
     9361 ttgtggtccg aaggtcaggc gtaaaatcac cagcagcacc gcaatgctaa aacctccaat
     9421 cgcaataatg ctgccgagca gactcagcat acgtagcggc gttgtggtaa ggcaggtcac
     9481 caggtcgtac atcaaattaa tcaggcgcat aaaactgtat ttggattcac caaactcacg
     9541 ctcggaatga tgtactggaa tttcaatggc acggcgggcg aagatattcg ccagaatcgg
     9601 gataaaggtg ctacgttcat ggcagtgcaa catcgcatcg acaatatgac ggcgataggc
     9661 gcgcagcata caaccgtagt cacccatcgc tttgccggtg gtgcgctgaa taagccggtt
     9721 aatcatcttc gaagctgttt tacgaaacca gctgtcctgg cggttctggc gtacagtccc
     9781 taccacgtcg taaccttcat cggcttttgc caccaggcgg gggatttctt ctggcggatt
     9841 ctggagatcg gcatcaaggg taataattaa gtcgccggta acgtgactga atcccgccat
     9901 aatcgctgaa tgttgcccgt aattgcggtt aagcaaaata gacacaatat ggctgttctc
     9961 cgcttgtgag gcttcgacca gcatatgcgc ggaattatcg ctactgccgt catcaatcag
    10021 caggatctca tactctttcc ccaacgattc acaggctgtg gtggtgcgcc tgattaattc
    10081 cggtaagctt tcctgctcgt tataaacggg aataaccacc gagactttct taacagggtg
    10141 gatttcaaac atggcttatt gtcctgcgag ttgctgaagg gctgtgatga cacggtcggc
    10201 atcggcggta gtcatatccg ggaacagcgg caaagaacag atgcgttcgc tattccattc
    10261 ggtattcggt aacgacagcg tgggaaaacg ctcgcgataa tatttttgtg tgtgagcggc
    10321 gcggaaatgt aacccggtac caatgcctct ttcttttaac gcttccatca acgcatcgcg
    10381 actgatacca caacgttgtt catcgacacg aataataaac agatgccagg cgtgaacgtg
    10441 cggccaggcg ggaaggctta atggctgaaa ggggagagct gccagtgctt gctgatattg
    10501 ctgggcaatt tcgcgccgac gggtgttgag gtgctctaat tttgctaact gtgtcagggc
    10561 aattgcggcg ttaatatcgg tcagattgta cttatagccc ggtgttaaga cttcagcctg
    10621 cggtgcacgg ccccaggttt gtctgtcata ggcatcgaca cccagaccgt gaaatttcag
    10681 catccgtagc tggcgggcaa ggttttcatt atcagttaca atcaggccac cttccgcaca
    10741 ggtaatattt ttaatggcat gaaatgaaaa aatagtggta ccttttgcgc caatatgtcg
    10801 ccctttgtaa tacgtaccga cggcatgggc agcatcttcg ataactgcga tgccgtaacg
    10861 ttcgccaatg gcgcgaatgg cgtcaatatc tgctggcgca ccggcataat gcaccggaat
    10921 gatggcttta gtgcgtggcg ttatggctga ctcgatcgct tcaggcgtga ccatcggcgt
    10981 atcgcggtcg acatccacca ttaccggcgt tgcacccaac aaggaaatca tattgagggt
    11041 tgaaacccag gtcagggaag gcgtaatcac ttcatcgccc ttgccaattt ccaacgccat
    11101 tagcgtgata tgcattccgg cggtggctga actgaccgcg atggcatgct gatttcccgt
    11161 caactggcaa aaggcttgct caagcgcctg attcttcgga ccggttgtga tccaaccgga
    11221 ttcgagaacc tctttaactg cagcgagttc ctccacgccc attgctggtc gcgaaaaagg
    11281 caaaaattct gacattgctt ttccttccgc cattgaatac ctgtccactt atatttgcta
    11341 taaagagtgt tgtgtatatt ttgccatttg
//
LOCUS       DAZGLJ010000146        11228 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000146 DAZGLJ010000000
VERSION     DAZGLJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.146"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            55..1833
                     /locus_tag="ACHK49_003409"
     CDS             55..1833
                     /locus_tag="ACHK49_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO9264003.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1873..2352)
                     /gene="xdhC"
                     /locus_tag="ACHK49_003410"
     CDS             complement(1873..2352)
                     /gene="xdhC"
                     /locus_tag="ACHK49_003410"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO9264004.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2349..3227)
                     /gene="xdhB"
                     /locus_tag="ACHK49_003411"
     CDS             complement(2349..3227)
                     /gene="xdhB"
                     /locus_tag="ACHK49_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO9264005.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3238..5535)
                     /gene="xdhA"
                     /locus_tag="ACHK49_003412"
     CDS             complement(3238..5535)
                     /gene="xdhA"
                     /locus_tag="ACHK49_003412"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO9264006.1"
                     /translation="MEAREATATGESCMRVNAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5950..6705
                     /gene="actS"
                     /locus_tag="ACHK49_003413"
     CDS             5950..6705
                     /gene="actS"
                     /locus_tag="ACHK49_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO9264007.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6784..6857
                     /locus_tag="ACHK49_003414"
     tRNA            6784..6857
                     /locus_tag="ACHK49_003414"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6816..6818,aa:Gly,seq:ccc)
     gene            6992..8599
                     /locus_tag="ACHK49_003415"
     CDS             6992..8599
                     /locus_tag="ACHK49_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9264008.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPSASYEELREHLKTIVEWELGVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLKGDNSGLLSYLE
                     PETGSLRPSVSLSVSSEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTAGKPYHYYVTALAAITGARLNEVAQLQVKDVRATEAGTVYIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTAGDNAMVFDGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVDRLVNVMAGVYKETGLNG"
     gene            8592..9251
                     /locus_tag="ACHK49_003416"
     CDS             8592..9251
                     /locus_tag="ACHK49_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFO9264009.1"
                     /translation="MAEYYPAVFEAEAFNCPHCGVYARQFWRRMYGSARELAQRTTEF
                     RMSTCSHCGDDAYWYDGNMIIPAAGNVELPNPDMPDNCKSDYMEARSIINLSPKGAAA
                     LLRLCLQKLMVHLGEPGENINKDIRSLVQKGLPVRIQQAADICRIVGNQAVHPGEISL
                     DDDPQLAHGLFKLLNIIVDDRITRPKEIEAMFQSMPEGPRQGIENQDRQAREQQQAAN
                     E"
     gene            10160..10888
                     /locus_tag="ACHK49_003417"
     CDS             10160..10888
                     /locus_tag="ACHK49_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO9264010.1"
                     /translation="MSQQEISVINLDQLVSMTSVEIAELTGKEHRNVLRDIRNMAEEL
                     NALKTEQCSKLSSHIGVTEDVYLNAQGKQQPLYRLDRKHTFILVAGYSVHLRAKCYDH
                     IQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKN
                     PISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPAIVDKLRGILIA
                     FTGTDQQKQEHLLKQAQSGKLEGL"
     gene            10890..11090
                     /locus_tag="ACHK49_003418"
     CDS             10890..11090
                     /locus_tag="ACHK49_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264011.1"
                     /translation="MIDPRTPIGRATLRYRGLPTRHLLSLLRLGVDNPDRPYYSRDEL
                     IAMLVDRDLNNQLRRAFAKLES"
BASE COUNT         2841 a         2755 c         2856 g         2776 t
ORIGIN      
        1 gaaccatcaa cgtcttcaat gatcgacttt taagtaagtc tggaaggtaa agctatggag
       61 cttgctacta cgcagtcagt attgatgcaa attcaaccga caattcagcg ttttgccaga
      121 atgcttgcca gcgttttgca gcttgaggtt gagatcgttg atgaaaactt gtgtcgcgtc
      181 gccggaacgg gcgcgtatgg gaagtttctt ggtcgccagt tgagcggcaa ctcacgcctg
      241 ctccgccacg tcctggaaac gaaaactgaa aaagttgtga cacagtctcg cttcgatccc
      301 ctttgcgaag gttgcgatag taaagaaaat tgccgtgaaa aagcatttct gggtacgcct
      361 gtcattttac aggatcgttg tgttggggtg ataagtttga ttgccgttac ccacgagcaa
      421 caagagcata tcagtgataa tttacgcgaa ttttctgatt atgttcgcca tatatccacc
      481 atttttgttt cgaaacttct ggaggatcag gggccaggag ataacatcag taaaatattc
      541 gcgaccatga tcgataatat ggatcagggc gtattagttg ttgatgatga aagtcgggtt
      601 cagtttgtta atcagactgc cttaaaaaca cttggtgttg tacaaaataa tattattggg
      661 aaacctatcc gtttcagacc attaacattt gagagtaatt ttactcatgg acatatgcag
      721 catattgttt cgtgggacga taaaagtgaa ttaatcattg gtcaattgca taacattcag
      781 ggccgacaat tatttttaat ggcatttcac caatcgcata ccagtttttc tgtagcaaat
      841 gcacctgatg aaccacatat tgaacaattg gttggcgagt gccgtgttat gcggcaatta
      901 aaacgactca ttagccgtat tgcacccagc ccatccagcg ttatggtggt tggtgaaagc
      961 ggcacgggta aagaagtcgt cgcccgagca atccataagt tgagcggaag acggaataaa
     1021 ccctttattg ctatcaactg tgccgcgatt ccggagcagc ttctggaaag cgaactgttc
     1081 ggttatgtta aaggcgcatt tactggcgct tctgccaacg gtaaaacagg gttgattcag
     1141 gcggcgaata cgggcacgct gtttctcgat gaaataggtg atatgccatt aatgttgcag
     1201 gctaaattac tgcgcgctat tgaggcgcgt gaaattctac cgattggtgc cagtagccca
     1261 atacaagtcg acattcgcat catttctgca actaatcaga atttggccca gttcattgcc
     1321 gaaggtaaat tccgcgaaga tctcttctac cgacttaatg ttatcccgat aactctgcca
     1381 ccgctgcgtg aacgtcagga agatattgaa ctattggtgc attacttttt acatctgcat
     1441 acccgtcgtc tgggatcggt ttatcctggc attgctcccg atgtcgtcga aatattgcgt
     1501 aagcatcgtt ggcccggaaa cctgcgcgag ttaagcaatt tgatggaata tctggttaac
     1561 gtagttcctt caggtgaagt tatcgacagc acgctattgc cgccaaatct gctgaataat
     1621 ggcacaacgg agcaaagtga tgtaacagag gtcagtgagg cgcacctgtc actcgatgat
     1681 gcgggcggca cggcgctgga ggagatggaa aagcaaatga tccgcgaggc gctttcacgt
     1741 cataacagca agaagcaagt tgctgatgaa ctgggcatcg gcattgctac gctctatcgc
     1801 aagattaaga aatatgagtt gttaaacaca taacctcagt gaatccggcc tgaattcagg
     1861 ccggatatcc ttttacttcg ttttctcgca atccagaact gtatttacaa tcatctgata
     1921 ccccgtgcag cgacaaagat ttcccgccag tccgcgacga atttccgtaa tggttaatgg
     1981 cttctcgcgt ggtttcgcca gcattgccgt ggtagccata atcaggccag gcgtacaaaa
     2041 cccgcactgc actgcgccgg atttcgcata agcctgctga acatgagaaa gttttccgcc
     2101 tttcgcttca ccttccagcg tgcggatctc ttttccttca gcccaggcgg caaggtataa
     2161 gcaactgtct attgctgtgc cgtcgaccaa caccgtacag gcaccacatt cacccacgca
     2221 gcacccttgt ttgacactta gcagtccttg ttcgcggagt aattccgaga gcggcgtgcc
     2281 tggtgcggcg tgaagctgaa aaggcatccc gttaatggtg cattcgatgg taattgtttc
     2341 gctgtgattc attgcaattt tccccccgcc gcggcgacgg cttcgctaat cacttttttg
     2401 gtcatcgtct ggatgagatg cagacgaaac tctttactgg cccgccatga agaacgcggg
     2461 gcgacatctt gcaggacaga ttcgctgata gcttccagcg tttgcaggtt taatggcgca
     2521 ttttgtgcag tctgttcggc atgttggcag cgaatcggcg ttggcgcggc aacaccaaat
     2581 gccaggcgta attcgctgaa attgccgtta tccagtcggc aatgtgcggc gcagccaatc
     2641 gttgaaatat ccattgcgtc gcgcatggca tatttaaaat gcgcgctgcc cgcgtgttct
     2701 ttcggctgtg gcggaaaatg aaaggcgacg aggatttcgt catgctcaag agacactttg
     2761 cccggcccgg tgtgaaagcc attaatcggg acgaaacgaa caccgcgcgg ggagtggatc
     2821 tccagtttcg cgtcataaat tagcgttggc gtggcagaat ctgcgctggt ggcaccgttg
     2881 caaatatttc caccgtaggt agcgacgtta cggatctgcg gtccagcaat ggacgtggcc
     2941 gcagcacata acgccgggag atgacgttga gttataggat cttctattag ctgggtaaat
     3001 gtcgttgcag agccgatacg tagcgagcca tcttccgcca gcgtaattcc ccgcagctcc
     3061 gccagattat gaatatcaac aatatggcga taacggtcat tgtgatggtg gagctgaatc
     3121 agtacgtcag tgccaccggc gagcagtttg gcctgcgggt tgtcagccag caggttgatg
     3181 gcatcggcaa gggttgctgc gcgatggtaa gaagcaaaat caaacatgat gttatcctca
     3241 aatcaatcct gccagatgga actcttcata taaccgtttt ggcgtcagcg gcagtgtatt
     3301 gattgcaaca ccggtagcca tcttcaccgc gttacgaata gcagcggcaa caggaattat
     3361 tggtggctca cccagtgact tatgtccgta tgcggattgc ggctcattga tttcgacgaa
     3421 cgcgctttcc agttgtggca gatccggcat ggtcggcatt ttgtaatcca gcagattggg
     3481 gttacggacc acgccgcttt tagcatcgat gatcatctct tcaaatagcg cccagccaat
     3541 gcccattccc attccgccgt gtacctgacc ttctgccagc agtggattaa gaatatgccc
     3601 tgaatcatga acgttgagga tgcggttgat ggtgactttg cacagcgcaa tatcgaccgt
     3661 cagatcaaca aaggtacagc caaacgccgg tgggttagtg gtggttttga tggagctttc
     3721 agcagagagc tgcccgccgc gttcagggtg gtagaaagcg tccatcgcca aatcttttaa
     3781 cgacattaac ggctcttccg gtcgttcaac cagcacgata tggcctttta tcagggtcag
     3841 attcatcgct gactgatgta gcatgactgc ggcgtgagcg atgattttct cttttaataa
     3901 tagtgccgca ctgcgcagcg caggcgcggc aacatagctc tggcgtgagg caaatgcgcc
     3961 gggatcgaac ggcgtaacgt cggtatcttg agttgaaata acgcgaacgt cgctgaccgg
     4021 aacccccacg gtttctgcca ccatttgcga gaagacggtg tcggcaccct gaccgatttc
     4081 cgtcgcgccg ctttgcacgt tgatggttcc atcctgattc atcagaaggc gcgcgcctgc
     4141 tatttctacg ccgacaggcc aggtgttaga ggtgtagcta aaacaggcga cgccaacgcc
     4201 gcggcgcaaa ttgccttgct ggttctggca ttctgcacgg cgtttttccc attcaaagat
     4261 tttccggcct ttttcaagac actccggcaa ccctgcgctg taaatacgtt tgcccgtgag
     4321 cggattagca tctccttcgc gggcggcgtt gcgtaaacga atttcaacag gatcaatacc
     4381 taacgctgtc gcggcgtcat caagcataga ctcaacggca aatacgactt gtggcgcgcc
     4441 ataaccacgc atcgcaccag ccgaggggag gttggtatag caggtctttg aactgtaagc
     4501 gtaggcacaa cgaggataaa ggtaagcgac tttattcccc cccgcagaag cgatggagtg
     4561 cccgtgagat gcataagcgc cggtgttaga cagaacatcc agactataac ctttcaatgt
     4621 tccgtcgcgg ttcacgccca tttgcccgtc aatggtaaaa gcgtggcggg tacgggttgc
     4681 gaggaaacac tcttcacggc taagggaaac tttcaccgga atgccgccaa gcttgctggt
     4741 caggaatgcc gccattggct cttccagtac atcctgttta ttaccaaaac cgccaccgac
     4801 aaacggtttg atgactcgta cgcatgacca gggaatatcc agcgcctgac caaccacgcg
     4861 gcgaacaatg tgcgggatct gggtgctgga aacgatggta attcgcgagt catcctccat
     4921 ccacgccagc gatgttacgc tttccatgtg acaatgttga ataacggggg tctgatagtg
     4981 cccctgtacc tggtagtcgg cggcatcgat tgtttgttgg acattacccg tcgacatcgt
     5041 gctttgtttc agtaaattgc caccgttatg gattggtgca gcgtcttctg ccagcgccgc
     5101 ttctggcgtg gtgataacgg gtaattcttg ccactcaatg ctgaccaatt gcgccgcttt
     5161 ttctgccgtg agttcatcgc gggccacgac gatggcaacg gcgtcaccat gatgacgaac
     5221 atggcgagtt agcagtgcgc gatcggcggt atcgcgcttg ttttcgtcaa gtgtccaggc
     5281 atgcccagca gtagcaaatg gaatatcagg cacatcttcc caggtaaaaa tcgccagtac
     5341 gcccggcaaa cttctggctt gttcatcatt aatacttacg gcataaccat gtgcgatagg
     5401 gctacgtaca tatttcgcat aacacatgcc cgccataaca taatcgtcag tatatcgtgc
     5461 ccgcccggtg accttagcaa tggcattgac gcgcatgcat gattcacccg tagcggttgc
     5521 ttcccgcgct tccatagaaa tcccctcgat ttgaacattt ggttcttatg gcaaagattt
     5581 agtctaagca ggcaagattt acgccagaaa cgacgataag cgttagggga ggtaggaagt
     5641 gtgatgacgt taaaaaaaca cagatatgat acgaaacagg actctgaata ggggcagtga
     5701 tacgtatcaa tatgagtggc gataaaatca atgtgataaa ttgggattat tacgcatcag
     5761 gatgggaaat agggtaaaaa gtcaggaagt tcgatacctc tacagcgaat attttgctga
     5821 tgatacttcc ctttgccaaa tgaaattaac ctctttctct attaccacgt ttttccagaa
     5881 gcaagagatt gggtatcaag aggctggctg ctatgataag gcgtcttgtt ttttaagcga
     5941 ggaaagattt tgagtgcggg acgcctgaat aaaaaatctc tgggtatcgt gatgttgtta
     6001 tcggttggac tgcttttggc gggctgttcg ggtagcaaat catccgatac aggaacgtat
     6061 tccggctccg tttacaccgt gaaacggggg gatacgctat atcgtatttc gcgcaccacg
     6121 ggaaccagcg taaaagaact ggcgcgactg aacggcattt ccccccctta caccattgaa
     6181 gttggtcaga aactaaaact gggtggggcg aaaagtagca gtagtacacg taaatcaacc
     6241 gccaaatcaa cgaccaaaac cgcatcggtt acaccgtcat cagcggtacc gaaatcttcc
     6301 tggccgccag tagggcaacg ttgttggtta tggccaacga cagggaaagt tatcatgccg
     6361 tattcgacag cagatggcgg caataaaggg attgatatct cagctccacg gggtacacct
     6421 atttacgccg cgggtgcagg aaaggtggtg tatgtgggca accagctgcg tggctacggt
     6481 aatctcatca tgattaaaca cagtgaagat tacattacgg cttacgccca taatgacacg
     6541 atgctggtaa ataatgggca aagcgtgaag gctgggcaaa aaatcgccac tatggggagc
     6601 acggatgcgg catctgttcg cctgcatttc cagattcgtt accgtgcaac ggcaattgat
     6661 ccgctacgtt acttgccgcc gcagggcagc aagccaaaat gctgatggcg aattaatcag
     6721 cagtcagcag cgcggcactt gctaaggaga gcgtaaggtt tataatgcct tacgcatctc
     6781 gaagcgggcg tagttcaatg gtagaacaag agcttcccaa gctctatacg agggttcgat
     6841 tcccttcgcc cgctccatga ccattcttaa agtctactaa cttactgaat cctaagggat
     6901 acggcttaaa gccttccgat agttttactc actgtaacca acactgtgac taactttgtt
     6961 gaagtagtga gcagacctaa ggtctcagta catgctcaat tcaagaacct acctttatca
     7021 gcgtaacggt gtcttttaca tccgtctcag gatgaagacc actggtcgcc tgaccgcttc
     7081 gcttccttct cacaaccggt ataaactagc gtcagtatca ctacggacta aggacagacg
     7141 taccgctatg gctcactcac ggcacatcaa gtcagcactt aaagcaatac acgcagacaa
     7201 ccctagcgca tcatatgagg aactacggga gcatctgaag accattgttg agtgggaact
     7261 tggtgtcagc cgtgatgacc tgaacgatcc ggagtcctac cagctttacg ttgaccagta
     7321 cgacgacatc aagtccaacc ttcgggaagc tgtagcgact gagcgtctga ctgtagacca
     7381 gcatcgctat atcaatgacg ttatcggtgt acttaaagcc tgtcaggata gactcaaggg
     7441 cgataactct ggcctgttgt cttaccttga gcctgagact ggcagtctaa gaccgtccgt
     7501 ctccctatct gtatcatcgg agcctgaagt ccctgaacct aaagcactga ccctcgcgtc
     7561 tctcattgag cagtacgagc aggagaacgc ccagaactgg aagccagcga ccctaagcga
     7621 aaaccgagcg tcacactcta cgctaatcga gatattcgac tatctggata ttcaggatgt
     7681 cggcaaggcg acccgtgcag atatgcttag ggtccgtgaa gtcctccaac agctaccgaa
     7741 gaaccgcaag caacgcttta agtctatgcc gctgtctgac ctgctgaacc gggagtctaa
     7801 gactgactgt cttgatgtcg ttaccatcaa caataaatat cttatcaaga tggctgctgt
     7861 ctttaagtgg gcggtacgta acgacctgat agctaagaac ctgaccgaag gtcttgagct
     7921 gaaagtcccg cagcgtaaag cctccgacgc tcgtgatgct ttcagtcctg agcaggtagg
     7981 gcaactactg gtcgccgcta aagcgtactc tcagaagacc gcaggtaagc cataccatta
     8041 ttacgtcacc gctctggctg cgattacagg tgcgagactt aacgaggtgg cccagcttca
     8101 ggtcaaagac gtcagggcta ctgaggccgg gaccgtatat atccatatca acgaagacga
     8161 tagcagccta ccgggtaaaa gtattaagaa cgctcatagc gaccgctgtg tcccgctggt
     8221 cgatggcgct tatggttttg tattggctga ctttatgtcg cttgtggaag accgtagaaa
     8281 gactgcgggt gataatgcta tggtattcga tggcctcaag ctgatgaaga atggctacgg
     8341 tgagcaggtg agcaaatggt ttaacagaac gttgctgcct aaagtccttg ctgaccgtag
     8401 cggcttagcc tttcactcat tcaggcacac tgttgcgacc cagttaaaac aacatggggt
     8461 cgagttagcg tatgctcagg cgattatggg tcatagctca gggtctataa cttacgaccg
     8521 ttacgctaaa gaggtcgagg tggatagatt ggttaatgtg atggcaggtg tttataagga
     8581 gactggatta aatggctgag tattatccgg cggtgtttga agcagaggct tttaactgtc
     8641 ctcactgtgg tgtatatgcc cgtcagttct ggcgtaggat gtatggaagt gcgcgtgagt
     8701 tggcgcaaag gacaaccgag ttccgaatgt caacatgctc tcattgcggt gatgatgctt
     8761 attggtatga cggcaatatg attattccgg cggctggcaa tgttgaacta ccaaatccag
     8821 atatgccaga taactgcaag tccgactata tggaagcccg aagcatcatt aatctgtctc
     8881 ctaaaggggc cgctgcactc cttcgtcttt gccttcagaa gctaatggtc cacttaggcg
     8941 agccgggaga aaacattaat aaagacatcc ggtcactcgt ccagaaagga ctacctgtac
     9001 gcatccagca ggcggcagat atctgtcgta ttgtcggcaa tcaggcggtc catcccggag
     9061 agattagtct tgatgatgac ccgcagttgg cacacggtct ctttaagctg ctgaacatta
     9121 ttgtcgatga ccgaatcacg agaccgaaag aaattgaggc tatgttccag agtatgcctg
     9181 aaggtccacg tcaaggtatt gagaatcagg acagacaggc gagggagcag cagcaggccg
     9241 ctaacgaatg aaacccacac tataggggaa ggggggggac taaactatca ctatagggga
     9301 gagaccctaa gtccccccgg gcttaataca gccaccgacc cacacattca ttaagtctca
     9361 ggcttccggt agcggaccga ttggaaacca tccgcaataa gcctagtgtt aaatcacctt
     9421 ggcctgagac cattcccttt aacgtagcaa tctctctcct ttgacaggcg gagaacatca
     9481 gaccgcagcg gaagtatccg tctatgcgac catcggtgtg cgagattgtg aagaaccaag
     9541 acatcattgc tgaggtctgc catccatttg tggacaggcg gaaactcaaa aaggttagac
     9601 cagtggtgtc tgaggttgga gctttgagcc tgaccagcag gcttgaggag accatgagtt
     9661 catcttgtta tggattgtgt tatcacaatt ccaataagaa gaactttaag tccagcctcc
     9721 agtcctgtca gcccgaagta tactacggtc gctatgatgt ggttctccat acttcgggtc
     9781 taactggaag tactgaacac tttcgcgtca ggttctccga agttcattcc tgtttgacta
     9841 aactatcact atagggacca tatctcgcct ctgacctcct gagagacggc aagcgagaaa
     9901 ccgtggtcca cgctagcttg ctagccacca gcctctggtc agcctttgtg ttctttggtc
     9961 tcgtgactta agtctatcac cacacacgct cggacatgct gtcgaacctt catctctatg
    10021 gtcatcgcag accactaacc ccaattacct caagtcattc tgtttgcgtc ctgttgcctg
    10081 ctggtctccc tttacgtggg ggcttgagta ggtcagtggg ggtgcgactt cagggtgcaa
    10141 acaaaaggag tacacgtaaa tgtctcaaca agaaatcagc gtcattaacc tcgaccagct
    10201 tgtctccatg acctcagttg aaattgctga gctgacaggc aaggagcacc gaaatgtcct
    10261 acgtgatatt cgcaatatgg ctgaggagct taacgcgctc aaaactgagc agtgctcaaa
    10321 actgagcagt catatcgggg tgactgaaga tgtctatcta aacgcgcagg ggaaacagca
    10381 gcctctgtac cgtctcgacc gtaagcatac tttcattctg gtcgctggct attccgttca
    10441 cctccgcgcc aagtgttacg accatattca gacgttagag cgtcgagtcc ttcaactgga
    10501 agaccagaag aagcgagcag ccattcagtc cgctaaccgc cgaggcgtga cttggggcga
    10561 ctactgcaaa acctatggtc tacccgctca gaagctaatg accgcacttc ttcagcatcg
    10621 tggtctcttt cgtaagaacc caatcagtaa cgagtggtct gtaaatccaa agtattccga
    10681 ctgcttccgt atcattaagc cgtcagacca gaagttctca gcaggtggtt ataactttcg
    10741 cttcaacgct aagggattgg aggtcttcgg taagcctgcg atagtcgata aactgcgagg
    10801 catccttatt gccttcactg gcaccgacca gcagaagcaa gagcatctcc tgaagcaagc
    10861 ccaatccggc aagctggagg gactctaaga tgattgaccc gcgcacacct attggtcgtg
    10921 caactctccg ctaccgtggt ctgcctaccc gtcacctcct gtccctgctg agacttggag
    10981 tcgataaccc tgatcgccct tactacagtc gagacgagct gatagcgatg ctggtcgacc
    11041 gtgatttgaa taatcagtta cgtcgagcct ttgcgaaact ggagtcttaa catctccgga
    11101 aatttccaga ccttaaccaa ccttatttaa aaccttttga gatgaccgat gcggggagac
    11161 ccgtaggcat tactgtgtct gcgataccga tagtctcaac tctcaagagg agtaattact
    11221 atgtctag
//
LOCUS       DAZGLJ010000147        11117 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000147 DAZGLJ010000000
VERSION     DAZGLJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.147"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(30..989)
                     /gene="ybtA"
                     /locus_tag="ACHK49_003419"
     CDS             complement(30..989)
                     /gene="ybtA"
                     /locus_tag="ACHK49_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HFO9264012.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHDGFNHEQKPSTLWLQGQSLVMLSLVLDEQREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            1246..2958
                     /gene="ybtP"
                     /locus_tag="ACHK49_003420"
     CDS             1246..2958
                     /gene="ybtP"
                     /locus_tag="ACHK49_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HFO9264013.1"
                     /translation="MALAGLAALTSLGALLFLVWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLQTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSEQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFVDTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            2945..4747
                     /gene="ybtQ"
                     /locus_tag="ACHK49_003421"
     CDS             2945..4747
                     /gene="ybtQ"
                     /locus_tag="ACHK49_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HFO9264014.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIACPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVYNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWVHE"
     gene            4740..6020
                     /gene="ybtX"
                     /locus_tag="ACHK49_003422"
     CDS             4740..6020
                     /gene="ybtX"
                     /locus_tag="ACHK49_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HFO9264015.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFRREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFMMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     LHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            6048..7352
                     /gene="ybtS"
                     /locus_tag="ACHK49_003423"
     CDS             6048..7352
                     /gene="ybtS"
                     /locus_tag="ACHK49_003423"
                     /EC_number="4.2.99.21"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     Irp9/YbtS"
                     /protein_id="HFO9264016.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVMGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLSENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(7546..8461)
                     /locus_tag="ACHK49_003424"
                     /pseudo
     CDS             complement(7546..8461)
                     /locus_tag="ACHK49_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(8623..8698)
                     /locus_tag="ACHK49_003425"
     tRNA            complement(8623..8698)
                     /locus_tag="ACHK49_003425"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8663..8665),aa:Asn,seq:gtt)
     gene            complement(8799..9596)
                     /gene="mtfA"
                     /locus_tag="ACHK49_003426"
     CDS             complement(8799..9596)
                     /gene="mtfA"
                     /locus_tag="ACHK49_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO9264017.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            9690..9779
                     /locus_tag="ACHK49_003427"
     tRNA            9690..9779
                     /locus_tag="ACHK49_003427"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9724..9726,aa:Ser,seq:cga)
     gene            complement(10448..11098)
                     /gene="zinT"
                     /locus_tag="ACHK49_003428"
     CDS             complement(10448..11098)
                     /gene="zinT"
                     /locus_tag="ACHK49_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO9264018.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPIFQKKADADKTKTFAEIKDYYRKGYV
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         2457 a         2758 c         3125 g         2777 t
ORIGIN      
        1 gggaggagtt taggggggcg cgaccccggt tacatcccgc gtttaaaggt cgaaggagtt
       61 acgccaaact gtttctggaa ggcggcagag aaatggctgg cgttggcata gcccagatcg
      121 gccgccaccg tcatgacgga ggtattgccg ttggccaggc gtcttctggc ttcctgcatt
      181 cgttcagcct gaaacaggcc atagacgctg ttgttgaaca gaacgcggaa gcctcgctta
      241 attttgagaa cgctcagacc ggactctctg gccagctccg cgacgcccgg cgcttgcgtc
      301 agatcagcca gcagcagatc cttcgcccgc agcagtttct ggcgttccat cgggttgaag
      361 tggcaggaga ggcaactggc gtcttcgcgc tgttcatcca gcaccagact gagcatgacc
      421 agactttgcc cctgcaacca tagcgtcgac ggcttttgtt catgattgaa gccgtcgtgc
      481 atgcgcatca gcgcgtgacg caaggcctga gccgttaagt gggtttcagc attacaacgc
      541 tgctgctggc aacagcagtg ggagtcgatc ttattcttaa tgaccgcgct aatatccggc
      601 acccacagcg cgagggtttc agggctgaag gagagggtaa tgctctcgaa tcggccacaa
      661 taggaggcgc tgccgcgaca gtctggcgta tgggtaatgt aatggcgatt gcgcgcaagc
      721 tcccagtcgg tgtggcgccg gagtccctga atcgcaaagc gcgatgtgcc ttccaggatg
      781 cacgagaagt taatcctgcc cagatcgtcg tgtagctgca acgtctcagg ccgcgcaaaa
      841 tggcccctcc agagcaaaat atccatacct tcctgcaacg aacagcgcag cagttcacac
      901 tgagcaggaa tattgccatc gtttgcaggt ttcccgacca ccaactggtg aatagagatt
      961 tcagattgcg tttgcggtga ctccgtcatg acctggttat ctccctgtgg cttagaccaa
     1021 tgcgggtata aatagaaccg aatggggtag atgagttatt gataatggtt attattcaca
     1081 ttaattctca gaacgccacc ctgaattgtc gtttttggcg tccgtaaaaa tgcatcagga
     1141 aattcagtta ctcccatgtc atctcaatca tccaatactg aatcgctgtc ccgatttcct
     1201 ctctggcagg tgattacacc ggttcgcagg aaggttattt tagctatggc gctggcgggg
     1261 ctggcggcat taaccagcct gggggcgctc ctttttctgg tgtggagcct gcgtgacatt
     1321 cgcgcgacgc ctgacgctat tccggcctgg ccgctgggtg gcgtgatcgg ctgcgtggtc
     1381 ttaacttttg ttctgcgctt acaggcgttc aacacctctc attacgcggc ttttcatctg
     1441 gagaacattc tgcgcagcag gttagcccgt aaagcattgc agcttccgcc tggcgtgtta
     1501 cagcaaatgg gcagcgggtc agtggcgaaa gtgatgctgg atgacgtgaa gtcgttacat
     1561 atttttgtgg ccgacagcac gccgctctat gcccgcgcga tcatcatgcc gctggcgaca
     1621 atcgttatct tgttctggct ggactggcgg ctggcaatcg cgacgctggg ggtactggcg
     1681 tttggatcgg ttgttctcgt gctcgcccgc cagcgttcgg aagatatggc tcagcgttat
     1741 cataaggcgc gcgagcaggt tagcgcggcg gttattgagt tcgtgcaggc catgcccgtg
     1801 gtgagaacgt ttgatagcgg cagcaccagt tttttgcgct atcaacgcgc ccttgaagag
     1861 tgggtcgatg tgctccaaac ctggtatcgc aaagccggtt tttcagcgcg tttttccttc
     1921 tcgattctga atcctctccc gaccctgttt gtcctgatct ggtcgggata cggcctgttg
     1981 cactatggca gtttcgattt tatcgcgtgg gtggccgttt tactgctggg cagcggaatg
     2041 gccgaagccg taatgccaat gatgatgctc aataacctgg tcgcgcaaac gcgtttaagc
     2101 attcagcgta tttatcaggt tctcgcgatg ccggagttat cgctgccgca gtctgagcag
     2161 cagccgcaag aggcgagcat tacctttgag caggtgagct ttcattatcc gcaagcgcgt
     2221 actggcgccg cgttgcagga ggtgagcttt catgtgcctg ccgggcaaat tgtggcgctg
     2281 gtcgggccaa gcggcgccgg aaaaagcacc gtggcgcgct tgctgctgcg ttacgccgac
     2341 ccggacaaag gccatatccg tattggcggc gtggatctgc gtgatatgca gacggacacc
     2401 ctgatgaagc aactctcgtt tgtgtttcag gacaacttcc tttttgtcga cacgatagcc
     2461 aataacattc gtctgggcgc gccggatacg ccgctggagg cggtaatagc ggcggccaga
     2521 gtggcgcagg cccatgattt tattagcgct ctgccagaag gttacaacac tcgagtcggg
     2581 gaacgtgggg tatttctctc cggcggccag cggcagcgca ttactatcgc ccgggcgctt
     2641 ttgcaggatc gccccatcct ggtgctcgat gaggcgacgg cgtttgctga cccggaaaac
     2701 gaagcggcgc ttatcaaggc gctcgcggcg gccatgcgtg gccggacggt catcatggtc
     2761 gcgcatcgtc tctcaatggt gactcaggcc gatgtgatat tgctgttttc cgacggacag
     2821 ctcagggaaa tggggaacca tacgcaactg ttggcgcagg gcggcctgta tcaacggctc
     2881 tggcaacact atcagcaggc gcagcattgg gtgccgggtg gaacacagga agaggtggtg
     2941 gaaaatgaaa gacaataatc ctgcggataa cctggcctgg cgcgtcatct ggcgccagct
     3001 tatctccagc gttggcagtc aggccaggat gctgcgacgc agtatgctgg cgctgttgct
     3061 ggcggcattc atgcagggga tcgcctttgc ctgtctttat ccgatcattg atgcgctgtt
     3121 acggggagac gcgccgcaac ttcttaactg ggctatggcc ttcagcgtcg ccgcaattgt
     3181 gacgctggtg ctacgctggt atggcctggg ctttgaatac cgtggtcatc tggcgcaggc
     3241 tacccatgag ttgcgcctgc gacttggcga gcagttacgc cgcgtgccgc tggagaagct
     3301 ccagcgcggc agggcgggtg aaatgaacgc cttgctgctg ggcagcgtgg atgaaaacct
     3361 caattatgtt attgcgatag ccaatatttt gctgctcacc attgtcacgc cgctgacggc
     3421 gtcgctggcg acattgtgga tagactggcg gctggggctg gtgatgttgc tgatcttccc
     3481 tctgctggtg ccgttttatt actggcgccg cccggcgatg cggcgacaaa tgcagacgct
     3541 gggggaagcg caccagcgtc tgagcggcga tatcgttgaa tttgctcagg ggatgatggt
     3601 attgcgcacc tgcggcagcg atgccgataa aagccgggcg ctgctggcgc atttcaatgc
     3661 gctggaaaac ttacagaccc gcactcaccg tcagggcgct ggcgcgacga tgctgatcgc
     3721 cagcgtcgtg gagttgggcc tacaggtggt ggtgttatcc gggatcgtct gggtagtgac
     3781 gggcactctg aacctcgcct ttttgattgc cgccgtcgcg atgattatgc gcttcgcaga
     3841 accgatggcg atgtttatca gctacacctc ggttgtggaa ctgatcgcca gcgccctgca
     3901 acggattgag cagtttatgg cgatagcacc gcttcccgtc gcagagcaaa gcgagatgcc
     3961 ggaacgttac gatatccgct ttgacaacgt cagctatcgc tacgaagaag gcgacggcca
     4021 cgcgcttaat catgtttctt tgacgttccc ggcagccagt atgagcgcgc tggtgggtgc
     4081 ctccggcgca ggcaaaacta ccgtcaccaa actgttaatg cgctatgccg atccgcagca
     4141 agggcagatt tctattggcg gcgtcgatat tcgccgcctg acgccggaac agctcaatag
     4201 cctgatttct gttgttttcc aggatgtctg gctgtttgat gacacgctgc tggcgaatat
     4261 ccgtatcgcg tgcccacagg cgacgcggca ggaggtagaa gaggccgccc gcgcggcgca
     4321 gtgccttgag tttatttccc gtcttccgca aggctggctg acgccaatgg gtgagatggg
     4381 cggccagcta tcgggcggcg agcgccagcg gatttccatt gccagagcgt tattgaaaaa
     4441 cgcgccagtc gtcattctcg atgaaccgac tgccgcgctg gatattgaaa gcgagctggc
     4501 ggtgcaaaaa gcgatcgata acctggttta caaccggacg gtgattatca tcgctcaccg
     4561 tttatccacc atcgccgggg ccggaaacat tctggtgatg gaagagggac aggtggttga
     4621 gcagggtact catgcgcaat tgctctcaca tcatgggcgt tatcaggcgc tgtggcaggc
     4681 gcaaatggcc gcgcgcgtgt ggcgcgacga cggggtttcc gcgtctggag agtgggtgca
     4741 tgagtgatgt tcagtcgaat gtgaaaccgc tgacgttgac gaccgggcgg gtgatttttg
     4801 ctattgccgg cgtctatgtg acgcagagtc tggtatcggc gctgtctatg cagtccttac
     4861 ccgcgctggt gcgcgctgct ggcggttcgc tggcgcttgc cggtgcgaca accctgttta
     4921 tgctgccctg ggcgctgaag tttatttggg cgccgtggat cgagcgctgg cggcttccgc
     4981 ccggtagcca ggaacgccgt tcccgcatgt taatcctgcg tggtcaggtc gcgctagcgg
     5041 cgatcctgac tattgccgca gcgattggct ggtttaggcg agaaggggga ttccccgata
     5101 cgcaaatcgt cgcgttattt gttctgttta tggtggcagg cacggtcgcc tccaccattg
     5161 atatcgccag cgacggcttt tgtgtcgatc aactgacccg cacgggttac ggctggggaa
     5221 acagcgtgca ggtcggcggc agctatctgg gaatgatgtg cggcggcggg gtgttcatga
     5281 tgctgtcggc ggcatccggc tggcctgtcg ccatgctgat gatggcggtg ctgattatgg
     5341 cgctgtcact cccgctgtgg cgcattacgg agccgacgcg aacagcgact atcctgcatg
     5401 ttccggcgtt aggttatgcg ctaaggagga agcaggcgcg cctgggctta ctgctggtat
     5461 tgatgctgaa ttcaggcatg cggtttgtgc tgcctcttct ggcaccgctg ttgttggatc
     5521 atgggttgag catgtccgca ttgggcgcgt tgttcagcgg cggcaatatt gcagcgggca
     5581 tagcaggaac gctggctggc ggattgctga tgaaatacac ctcacccggc agagcgctgt
     5641 tgacggctta tggcgtccag gggatcgcgc tgctggcggt ggtgatgacg ctcatgatgg
     5701 cgccgggtca tctgctgctg cagattctcc agtgtctggt cattgtccag tccatttcgc
     5761 tggcctgcgc gctggtctgt ctttacgcca cgctgatgtc gctttcatcg cctttgcagg
     5821 ccggtgtcga cttcaccctc tttcaatgta ctgatgcggc aatcgccatt ctggcaggcg
     5881 ttatcggtgg cgttgttgct caacattttg gctatgcggc ctgcttcctg tttgccgggg
     5941 cattcacgtt gctggcggcg tgggttgctt atatccggct gcattcggca agagaactga
     6001 tgacaagcgc aattgattga tccgcgaata acacagagag gtaagggatg aaaatcagtg
     6061 aatttctaca tctggcgtta ccagaggaac aatggctacc gacgatttct ggcgttttac
     6121 gccagttcgc agaagaagag tgttatgtct atgagcgtca accctgttgg tatttaggca
     6181 aagggtgcca ggcacggctg cacattaatg ccgacggaac gcaggcgaca tttattgatg
     6241 atgccgggga gcaaaaatgg gcggtggatt ccattgccga ctgcgcgcgt cgttttatgg
     6301 cgcatcctca ggtgaaagga cgtcgggtat atggacaggt tgggttcaac tttgcggcgc
     6361 atgcgcgggg gattgccttt aacgccgggg agtggccgct gctgacgtta accgttcccc
     6421 gtgaagaact tatttttgaa aagggaaatg tcaccgttta tgcggactcc gccgacgggt
     6481 gccgacgttt gtgcgagtgg gtaaaagagg ccggtacaac gacgcagaac gcaccactgg
     6541 cggtggatac cgccctcaat ggtgaggcat ataaacaaca ggttgcgcgc gccgttgcgg
     6601 agatccgccg tggcgagtat gtcaaagtga ttgtctcgcg cgccattccc ctgccatcgc
     6661 ggattgatat gcccgccacg ctgttatacg ggcggcaggc aaacacgcct gtgcgctcgt
     6721 ttatgttccg tcaggaagga cgcgaagcgc tgggctttag cccggaactg gtgatgtcag
     6781 tgatgggcaa taaagtggtc actgaaccgc ttgcgggcac ccgcgatcgc atgggaaacc
     6841 cggagcataa taaggcgaaa gaggcagaac tgctgcacga cagtaaagag gtgcttgagc
     6901 atatcctttc tgtcaaagaa gctattgctg aactggaggc tgtttgccag ccgggcagcg
     6961 tggtggttga agatttaatg tcggttcgcc agcgcggcag cgttcagcat ctggggtctg
     7021 gcgtgagcgg tcagctttcg gaaaacaagg atgcctggga tgcgtttacc gtgctgtttc
     7081 cgtcgattac cgcctcaggt atccctaaaa atgctgctct gaacgcgatt atgcaaattg
     7141 agaagacgcc gcgagagctt tattccggcg caatcctgct gctggacgat acgcgctttg
     7201 atgcggcgct agttctgcgt tccgtatttc aggatagcca gcgctgctgg atacaggcgg
     7261 gggcgggaat catcgcgcaa tctacgccgg aacgcgaact gacagaaacc cgggagaaat
     7321 tagcgagcat tgcgccctat ttaatggtgt aggagtaact gttttctaac ctcacaacca
     7381 gactgtccaa agacaattga gtcgctcttt gattcctctg tgactctatt gtcttctttt
     7441 gggataaaat aggtgttttt cgtcatgaag ttgataaaag tctatataga accacctcct
     7501 gtggaggtgg tggtaatttg ccagagtgag ggattcgtta gtatactatg ggataaactt
     7561 atttttacca attgtataag gcggcgcata agattctcgg catgcttcca gataatccga
     7621 ccaccactgc atcatcgctt tgcgggcttc taggtgctct gccttatgaa tataagccat
     7681 gcgcacggta ttgcgctcct gatgactcat ctggcgttct actgcgtcct ttgcccataa
     7741 acccgattcc atcagcgcac tgcatgccat tgcccggaag ccgtgaccgc agatatcctt
     7801 tttcgtgtcg taacccatca cccgcagtgc cttattgacc gtgttttcac acattggctt
     7861 atacgggtta tggtcgccgg ggaagatcag ttcattatta ccggtgatat ccttaatctg
     7921 tttcagaatg gcgatggact gttctgagag ggggacgata tgcggcattc gcatttttgc
     7981 cccgcggccg gaataacgca cgccaataat gggttctcgc gtcgcgggta tcgtccagac
     8041 tcggtttgtg aaatcaatct ctgaccagcg ggcgaaacgc agttcactgg agcgaatgaa
     8101 cacatgcagc atcagcagaa cggcatgccg ggtcagttca cggccctgat gatatgcccc
     8161 aatacgttca agcagttcag gcagccgctc cagcggcagg gcaggatagt gccgtctgac
     8221 aggaggtgtg gtcacgccgc caaggtttgc tgcagggttc gtatcgatta actcctgatg
     8281 gaccgcatgg cgcattatgt tactcaggtg ctgccgtgtg cgggacgcaa cctccagctt
     8341 tgagatacca gtggcgggag ccacccggct tgaccagcag atacagaccg tgagaatcgg
     8401 agactttaaa gggtttatca gaaggcttga gggtgcggat ttttgcgtcg gtaaggaaca
     8461 tatgggggtc actccattat cgaactaacc tgatcccaga tttgaccacc aatttttccc
     8521 gatgcatagg gtaaaatcaa aatgcaccga gaagagtttt cacactaacc tgttgaatca
     8581 acatcagata aagatttgca aggatgcatg aaaacagaaa attggctcct ctgactggac
     8641 tcgaaccagt gacatacgga ttaacagtcc gccgttctac cgactgaact acagaggaat
     8701 cgtgtgaacg gagcgcatat tacttagcgg taccttgtct gtcaacacta aatttcataa
     8761 gccatttcaa ttggttaatt aatctgcaaa gttgttaatt aatgaacatt cgtcgccgaa
     8821 aacgagtctg tatcattagc gtgatgcagt ctctgcaaag gatcttgttg ataaaattga
     8881 cagaaacgtt gccacaatga agggaaacga ggagcaaaaa gttctggggc gctaaagaaa
     8941 tattcagaaa gtacggcaaa acattcagca ggatcactgg cagcataagc atcaatgctc
     9001 gctgcattct caccaactaa ttcgatttct tcctgaatgt tgttcattgc agcatgaaga
     9061 tcgtgttccc agccagcaac ctcacgcaac gaaataaagg gaactccgct ggcgcgatcg
     9121 ccgttacggg tgtccagctt atgagcgact tcatgaataa tcaggttaaa accagatgca
     9181 tcaaaagaat cttgtatatc caaccagttc aaaacgatag gcccttgctg ccagctctga
     9241 cctgactgaa caatacgttg gttatgcacc agaccgatat cgtcttccca ttcatcatcg
     9301 accacaaatg gcgcaggata aattaagact tcatgaaaac catccagcca ttccagtcct
     9361 aactccagaa cgggtaggca aaatagaagt gctatccggc agcttcttaa tgaatccagt
     9421 tcaaagccct gtaaaggaac aagccgcttt tgctgtaaaa aacgttcggc aagagtgact
     9481 aatttgcttt gttcctgttc cgtcagaccc gttaaaaggg ggatcgatag tgcttcctgc
     9541 cagggaaggg cagtttgatg tgctgattct tgtactttcc agggccactt aatcatcgtt
     9601 ttgctcgcaa actcgtcact tgaacaaaat tgcacggaca gggactgtta aaatgccaaa
     9661 tttcctggca tcatggcaac catctgaacg gagagatgcc ggagcggctg aacggaccgg
     9721 tctcgaaaac cggagtaggg gcaactctac cgggggttca aatccccctc tctccgccac
     9781 tttatcaatg acttatctcc tgacttcccg ccttgctttt cctaaacaga acaatcgtag
     9841 aatattcttg aagggttaga tcgtcactgt tttctgttcg atactgtgac attcagcact
     9901 tgattcgcta tggagctgac aggaaggttt cgagcgaaaa tctacagtta ttcagtcgtt
     9961 ttcttatcgg tcaccattat tcttttagac attgatccta tgaaactgcc gcaaagttgc
    10021 tggcgggaac tgaagttgcg tagagaaggg gtcaataatt gagtcaaaaa aggaacgcgg
    10081 ccgtcagatt aaagccgcgg gacaaagtcc ataatttcca agagttgttg aatttcccag
    10141 cttttttgaa agcctttgta tcatactaac aagtaaaaaa ggtttaaaca ccaaaattaa
    10201 cctatattac tggttagctt taccatgaca gatgacttgc tcctggaaac atttgcgctg
    10261 ccagtctgta tggctgagtt gtgtatttat tgttcgcctt tttcgcattt ttttctacag
    10321 acataataaa aaagacacga ctcaagtatg agcgtgtctg agtttgtctt acttatttgt
    10381 cttggcaaat tattgttgtt actaccaggc actgttctgt taaataactg aagtcagcgc
    10441 aaaaaaatca atgagacatc atctcctcga ccacttcttc gctactcaac tgatagggat
    10501 aatacgttgg ccagttttcc atttcattca ataaagactg ttgggaatca ttacccataa
    10561 aaatgtgaaa atgagatgat tttcgtggtg caattatatg atcgctaaat tgtatatatt
    10621 taggggcttt actttcagga tctttacatt cgaataagta acgaacgcct ttcttgcctg
    10681 atttataggt gagtattttg tatccatcgt aatcatattt acaggatgtt gtttcattat
    10741 ttctatggaa ttcgacaatg ccgtcctcaa tgccaatcat ctcgatgtct gtcacataac
    10801 ctttgcgata ataatcttta atttcagcaa atgttttggt tttatctgca tccgctttct
    10861 tctgaaagat ggggtcaagt ttgccacttt gcagtaaagg ataaacggat tgccagactc
    10921 catcccagtc actgagcgtt cggttttgta cattggcatc atcaaaaaca ccattagcag
    10981 ctttttgttc gacctctgtt aagggtttgc cgtgtgagtg atgaccatgc gaaaaggcag
    11041 gagcgctaac aataaagaca cctaaagcaa cagccagttt atgaagacga atcgccaaaa
    11101 cagtttcctc cagagtt
//
LOCUS       DAZGLJ010000148        11114 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000148 DAZGLJ010000000
VERSION     DAZGLJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.148"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(132..1961)
                     /gene="glmS"
                     /locus_tag="ACHK49_003429"
     CDS             complement(132..1961)
                     /gene="glmS"
                     /locus_tag="ACHK49_003429"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO9264019.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2123..3493)
                     /gene="glmU"
                     /locus_tag="ACHK49_003430"
     CDS             complement(2123..3493)
                     /gene="glmU"
                     /locus_tag="ACHK49_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO9264020.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3844..4263)
                     /gene="atpC"
                     /locus_tag="ACHK49_003431"
     CDS             complement(3844..4263)
                     /gene="atpC"
                     /locus_tag="ACHK49_003431"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO9264021.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4284..5666)
                     /gene="atpD"
                     /locus_tag="ACHK49_003432"
     CDS             complement(4284..5666)
                     /gene="atpD"
                     /locus_tag="ACHK49_003432"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO9264022.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5693..6556)
                     /gene="atpG"
                     /locus_tag="ACHK49_003433"
     CDS             complement(5693..6556)
                     /gene="atpG"
                     /locus_tag="ACHK49_003433"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO9264023.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YADTMRKVIGHLASGNLEYKHPYLEERDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6607..8148)
                     /gene="atpA"
                     /locus_tag="ACHK49_003434"
     CDS             complement(6607..8148)
                     /gene="atpA"
                     /locus_tag="ACHK49_003434"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO9264024.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLVDVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8161..8694)
                     /gene="atpH"
                     /locus_tag="ACHK49_003435"
     CDS             complement(8161..8694)
                     /gene="atpH"
                     /locus_tag="ACHK49_003435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO9264025.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8709..9179)
                     /gene="atpF"
                     /locus_tag="ACHK49_003436"
     CDS             complement(8709..9179)
                     /gene="atpF"
                     /locus_tag="ACHK49_003436"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO9264026.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9241..9480)
                     /gene="atpE"
                     /locus_tag="ACHK49_003437"
     CDS             complement(9241..9480)
                     /gene="atpE"
                     /locus_tag="ACHK49_003437"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO9264027.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9527..10342)
                     /gene="atpB"
                     /locus_tag="ACHK49_003438"
     CDS             complement(9527..10342)
                     /gene="atpB"
                     /locus_tag="ACHK49_003438"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO9264028.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10351..10731)
                     /gene="atpI"
                     /locus_tag="ACHK49_003439"
     CDS             complement(10351..10731)
                     /gene="atpI"
                     /locus_tag="ACHK49_003439"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO9264029.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2674 a         3022 c         2739 g         2679 t
ORIGIN      
        1 aaaaatcaag tttgttatca gacattaatg ataattcaga atatatttat atagaaagat
       61 agatgaattt attgatgtca gttataaaaa agggccaccg gtcggcagcc cttttttatc
      121 tatgatagtg attactcaac cgtaaccgat tttgccaggt tacgcggctg gtcaacgtcg
      181 gtgcctttga tcagcgcgac atggtaagcc agcagctgca gcggaacggt gtagaagatc
      241 ggtgcaatca cctcttccac atgcggcatc tcgatgatgt gcatgttatc gctacttaca
      301 aaacccgcat cctgatcggc gaagacatac aactgaccgc cacgcgcgcg aacttcttca
      361 atgttggatt tcagtttttc caacagttcg ttgttcggtg caacgacgat aaccggcata
      421 tccgcgtcaa tcagcgccag cgggccgtgt ttcagctcgc ctgcagcgta ggcttcagcg
      481 tgaatgtaag agatctcttt cagcttcaat gcgccttcca gcgcgattgg gtactgatcg
      541 ccacggccca ggaacagcgc gtgatgtttg tcagagaaat cttctgccag agcttcaatg
      601 cgtttgtcct gagacagcat ctgctcaata cggctcggca gcgcctgcag accatgcaca
      661 atgtcatgtt caatggaggc atccagacct ttcaggcgag acagcttcgc caccagcatc
      721 aacagcacag ttaactgagt ggtgaatgct ttagtggatg ccacgccgat ttccgtaccc
      781 gcgttggtca tcagcgccag atcggattcg cgcaccagag aagaacccgg aacgttacag
      841 attgccagcg aaccaaggta acccagctct ttcgacagac gcaggcccgc cagggtatcc
      901 gcggtttcgc cagactgtga caaggtgatc atcaggctgt tacgacgcac ggcagatttg
      961 cgatagcgga attcagaggc gatttcgacg tcgcacggaa tacctgccag cgattcaaac
     1021 cagtaacggg aaaccatacc ggagttataa gaagtaccac aggcgaggat ctgaatatgc
     1081 tcaaccttcg acagcagttc gtcggcgttc ggtcccagct cgcttaaatc aacctgaccg
     1141 tggctgatgc gcccggtaag ggtgtttttg atcgcgttcg gctgttcgta gatctctttc
     1201 tgcatgtagt gacggtaaat acctttatcg cccgcgtcat attgcagatt ggattcgata
     1261 tcctgacgtt ttacttccgc gccagtttta tcgaagatgt ttaccgaacg gcgagtaatt
     1321 tccgcaatat cgccctcttc aaggaagata aagcgacggg tcaccggcag cagcgccagc
     1381 tggtcagaag cgataaagtt ttcgcccata cccagaccaa tcaccagcgg actaccagaa
     1441 cgtgccgcca gcagggtatc cgggtgacgg gagtccatga tcactgtacc gtacgcacca
     1501 cgcagttgcg ggatagcacg cagaacagcc tcacgcagag taccgccctg tttcagctcc
     1561 cagttcacca gatgggcaat tacttcggtg tctgtttcag aaacgaaggt atagccacgc
     1621 gcttttaact cttcgcgcag cggttcatgg ttttcgatga tgccgttatg caccaccaca
     1681 atgtgttcag aaacatgcgg atgcgcattc acttctgaag gttcaccgtg ggtcgcccag
     1741 cgagtatgag caatacccgt accgccatgc agaggatgtt cttccgctgc ctgagccagc
     1801 atctggactt taccgaggcg acgcaggcgg gtcatatgac cttccgcatc aacaacggcc
     1861 agaccggcag agtcatatcc gcggtattcc agacgacgta aaccttcaag aaggatttct
     1921 gctacatcac gttgcgcgat cgcgccaaca attccacaca tagtttttga ttccgattta
     1981 tatcgttgtc ggtcaacctg tatgcccgtt ttttcgggcg ccccgagcct tgtagagagt
     2041 ggggttattt ttatagttac tgcttgtggg agggagatta tgttatctcc tcatcccatg
     2101 tgaccggatt acccggccag aatcactttt tctttaccgg acgacgccag ccttctttct
     2161 gagtctgcgg cacacggctg atagctaatg cattttcgcc gacattacgc gtcacagttg
     2221 tacccgcagc aatggtcgcg cctttgccta ctgttaccgg ggccaccagc tgagtgtcgg
     2281 aaccgacaaa cacatcgtcg ccgataatgg tcttaaattt attcgcacca tcgtagttgc
     2341 aggtaatggt tcccgcgccg atgttaacgt tatcgccaat ttccgcatcg cccaggtaag
     2401 tcagatgacc agctttcgag cctttaccca gacgcgcttt tttcatctca acaaagttac
     2461 cgacgtgtgc accttccagc aactcagcac caggacgcag tcgggcaaac gggccaatag
     2521 tacaggccgc tgccagattc gcgtcttcca caacggtata cggactgatt tcgcaatcat
     2581 caccaatcac gctgttttta atcacgcaac cggtgccgat tttcacgcga tgaccgagag
     2641 tcacgttgcc ctcgatgata acgttagtat caatttcaac atcgcgcccg tgagtaagcg
     2701 taccgcgcaa atcaaaacgc gccggatcgc gcagcataac gcctgctaac agcagttttt
     2761 cagcctgttc ggactggtaa acgcgctcca gacgggagag ttgcaggcgg ttattcacac
     2821 cttctacttc gcttaaacgt tgcggatgaa cggcgacgat ttcacgccct tcctgatacg
     2881 ccagcgcaat aatgtcggtg atgtagtatt cgccctgagc attattgttg gtcagcttcg
     2941 ccagccagcg tttcatatct gcgccgttag cgatcagaat gccggtgttg atctcctgaa
     3001 tctgacgctg ctcgtcggtg gcatctttgt gctcaacaat gccggtaact ttgccatttt
     3061 cacgggtgat acgtccataa ccggtcggat catccagttt caccgtcagc agaccaatgc
     3121 caccctgcgg tttagcatca cgcaggcgct ggagtgtttc gacagagatc agcggcacgt
     3181 cgccgtagag cattaaaatg tcttcatcat cggcaaagaa aggtgcggcc tgctgcattg
     3241 catgacccgt acccagctgc tctgcctgaa gcacccagtt aaggttgtcg tctttcagcg
     3301 cctgtttaag cagatcgccg ccgtgaccgt acaccaggtg aacgtgcgct gcgcctaatt
     3361 cattcgcagc atcaatgaca tgctgaacca tcgctttccc ggcaagggta tgcagcactt
     3421 tcggaagatc ggaatacatg cgcgtgcctt tgcctgcggc aaggatcact acgctcatag
     3481 cattattcaa catacgcgtc ctgactgtaa tttgagaacg aatttaaacc gcttcacctt
     3541 gaaaaaacta catttttttc atcgtgaaat ggacagagga taaattgttc aataacggat
     3601 tatccccgct gacgaaagtg ccattttcga ccatcgtcac gccttttgtc tgcagaaaat
     3661 taaggcagta ccttcattta agcgtcaggt ggatgttttt gctcttattt cgatcaatga
     3721 ataaacggga aaacaggggt gttttatatc agcaggatct atgtgaacgc tcttcaggac
     3781 ggatcacgcg taaaaaaaag ccagcctgtt tccagactgg cttttgtgct tttcaagccg
     3841 gtgttacatc gcttttttgg tcaactcgat aacgcgcagc tgcgcgatcg ctttggccag
     3901 ttccgcagac gcctgagcgt aatctacgtc gccgtgagag ctgctaatgt gctcttcagc
     3961 cttacgtttc gcttccatgg ctcgcgcttc gtcgagatcc tggccgcgaa ttgcggtgtc
     4021 ggccagaacg gtcacgttgc caggctgcac ttcaagaatg ccgccagaca gatagataaa
     4081 ctcttcgtga ccgtgctgtt tcacgatgcg aatcatacca ggcttaatgg cggtgagcag
     4141 cggtgcgtgg ccagggtaga tccccagttc accttcgcta cccgttacct ggattttctc
     4201 gaccagacca gagaacattt gttgctctgc gctgacgacg tccaggtggt aagtcattgc
     4261 catatcaccc tccgattaag gcgttaaagt tttttggctt tttccacagc ttcttcgatg
     4321 gaaccgacca tgtagaacgc ctgctccggc aggtgatcgt attcgccttc catgatgcct
     4381 ttaaagccac ggatggtgtc tttcagggag acgtatttac ccggagaacc ggtgaatact
     4441 tctgccacga agaacggctg ggacaggaag cgctggatct tacgagcacg cgctaccacc
     4501 agtttgtctt cttcagacag ttcatccata cccaggatgg cgatgatgtc tttcagttcc
     4561 tgataacgtt gcaggatgga ctgaacgcca cgcgcggtgt cgtagtgttc ctgaccaacc
     4621 accagcgggt ccagctgacg gctggtggag tccagcgggt caacggccgg gtagataccc
     4681 agagacgcga tctgacggct cagtaccacg gttgcgtcaa ggtgcgcaaa ggtggttgcc
     4741 ggagacggat cagtcaagtc atccgcaggt acgtataccg cctgtacgga ggtgatagaa
     4801 ccagttttgg tggaggtgat acgttcctgc agaacgccca tctcttccgc cagggtcggc
     4861 tgataaccta ccgctgaagg catacggccc agcagtgcgg atacttccgt accggccagg
     4921 gtgtaacgat agatgttgtc aacgaacagc agaacgtcac gaccttcgtc acggaatttc
     4981 tcagccatgg tcagaccggt cagagcaacg cgcagacggt ttcccggcgg ctcgttcatc
     5041 tggccataca ccagggatac tttgtcgata acgttggagt cggtcatttc gtggtagaag
     5101 tcgttaccct cacgagtacg ttcacctacg cccgcaaaca cagagtaacc ggagtgctcg
     5161 atcgcgatgt tacgaatgag ctccatcatg tttacggttt tacctacacc cgcaccaccg
     5221 aacagaccaa ctttaccgcc cttagcgaac ggacacatca ggtcgataac tttgataccg
     5281 gtttccagca gttcctgaga gtttgacagc tcttcgtagg aaggtgctgc gcggtgaatc
     5341 gcccaacgct cttcttcacc gatctcgcct ttcatgtcga ccggttcacc cagtacgttc
     5401 atgatacggc ccagagtcgc tttacctacc gggacttcga tcgggtgttc gaggtctttt
     5461 acatccagac cgcgacgcag accgtcggag gaacccattg cgatggtacg cacgataccg
     5521 ccgccgagct gctgctgaac ttccagcacc agacgctcat taccattttg cacctcaaga
     5581 gcatcgtaca cgcgcggtac ggcatcctga gggaattcga cgtcaactac ggcgccgatt
     5641 acctggacaa tctttccagt agccatctta aatcctctac gaaataacct gtttaaaccg
     5701 cggcggcccc cgagacgatc tcggtgagtt cctgagtaat gctggcctga cgagctttgt
     5761 tgtataccaa ctgcagctct ttaatcaggc tgccgccatt gtcggtcgcg gctttcatcg
     5821 ccaccatacg ggcggcctgc tcgctggcca ggttttcaac cacgccctga taaacctgag
     5881 attcgacata acgacgcagc agggtatcca gcaacgcctt cggatcgggt tcgtacaggt
     5941 aatcccagga tttatgtttc agatcatcat catctgatgc cggtaacggc agcagctggc
     6001 tgatggtcgg aacctgagac atggtgttaa taaatttgtt gctgacaatg taaagcttgt
     6061 ccagacggcc ttcgtcgtag gcctgcaaca tcacttttac cggaccgatc agttcggaca
     6121 gggaagggtt atcccccatg ccggtgacct gggcaacaac attgccgccc acggagttga
     6181 agaacgacac gcctttcgag ccgatcattg cgaggtcgca ttgaacgcct ttgtcggtcc
     6241 aggtcttcat ttccgccagc agttttttga acaggttaat gttcaaacca ccgcacaaac
     6301 cacggtcggt cgacaccacc aggtagccca cgcgtttaac gtcgcgttct tccaggtatg
     6361 ggtgcttata ttccagatta ccgctcgcaa ggtgaccaat cactttgcgc atggtatctg
     6421 cataaggacg gctggccgcc atgcgatcct gcgatttacg cattttggaa gcggcgacca
     6481 tctccatcgc tttagtgatc ttttgcgtgt tctggacgct tgcgatctta ctacgtatct
     6541 cttttgcgcc ggccatgagc ttctcctcaa tgccttgcgg cctgccctaa ggcaagccgc
     6601 cagacgttac caggattggg ttgctttgaa ggaatcgagg atgcctttca gcttgccttc
     6661 gatttcgtcg ttgtagccac cggtctggtt gatctcttgc atcaacggag cgtgatcacg
     6721 gtcgacgtaa gccagcagag cggcttcgaa gctgccgatt ttcgacagtt caacatccac
     6781 caggtaacca cgttctgctg cgaacagaac cagagactgt tgcgctacgg acatcggcgc
     6841 atactgtttc tgtttcagca gttcggtcac tttctgaccg tggtcaagct gcttacgtgt
     6901 tgcatcgtca aggtcggatg caaactgaga gaacgctgcc agttcacgat actgtgccag
     6961 agcggtacgg ataccaccgg acagtttttt catgatcttg gtctgtgctg caccaccaac
     7021 acgggatacg gaaatacccg ggttaaccgc aggacgaata ccggcgttga acaggttggt
     7081 ttccaggaag atctgaccat cggtaatgga gattacgttg gtcggaacga acgcagaaac
     7141 gtcacccgcc tgagtttcaa taatcggcag tgcggtcaga gaaccggttt tccctttcac
     7201 ttcacctttg gtgaaggctt caacgtattc ggcgttaaca cgcgcagcac gctccagcag
     7261 acgagagtgg aggtagaata cgtcgcccgg gaatgcttca cgtcctggcg gacgacggag
     7321 cagcagggag atctgacggt aagcaacagc ctgtttagac aggtcatcgt aaatgatcag
     7381 cgcatcttca ccgcggtcac ggaagtattc gcccattgcg caaccggcat acggagccag
     7441 atattgcagt gcagcggatt cagacgcggt tgctaccaca acgatggtgt tagccagtgc
     7501 gccgtgctct tccagtttac gtaccacgtt agaaatggtg gacgctttct ggccgatagc
     7561 gacatagata catttgatac cggaatcgcg ctggttgatg atggcatcga tagccagtgc
     7621 ggttttaccg gtctgacggt caccgatgat caattcacgc tgaccacgac caattgggat
     7681 catggagtca acggctttat aaccggtctg taccggctga tctacggact gacgttcgat
     7741 aacgcccgga gcgattgctt ctacagcaga gaagccgtcg tgatccagcg gacctttacc
     7801 gtcgattggt gcacccagag tgttaaccac acggcccagc aggccacggc caaccggaac
     7861 ttccaggata cggccagtac acttaacttt catgccttcg gcaaggtcag cgtacggacc
     7921 cataacaacc gcacctacag agtcgcgctc gaggttcagt gcgatagcgt aacggttacc
     7981 cggcagggag atcatttcac cctgcataca atcggccagg ccgtgaatgc ggataacacc
     8041 gtcacttaca gaaacaatag taccttcgtt gtgagcttca ctcacaacat tgaactgagc
     8101 aatgcgctgc ttgatcagtt cgctgatttc ggtggaattc agttgcatgc tccagtcccc
     8161 ttaagactgc aagacgtctg caaggcgctc aagacgaccg cgtacgctgc catcaatgac
     8221 catatcaccc gctcggatga taacgcctgc cattacagac ttatcgattt tgcaattcag
     8281 cttaactttg cgtgacagac gtttttccat cgcagcagaa attttcgcga gctgttgttc
     8341 actcagtgcg gcagcggaaa tgacgtctac ctcagcggta gcctcactca cggcacgaag
     8401 gtgaataaac tgctccagaa catccgggag cgcgttaaga cgaccatttt cagccattac
     8461 ccgaatcagg ttctgaccgt tttcgtccag ttgctcacca caaactgcga taaacgactc
     8521 ggcgagcgtt tctggcgcaa gcgcgccaga gagaagctct gccatttgtt cgtttttggt
     8581 tacctcggcg gcaaacgcca gcatgtcctg ccagcgttct acactttggt gttcgacggc
     8641 aaagtcaaaa gctgctttgg cgtaggggcg agctaccgta ataaattcag acatcagccc
     8701 ctccctcctt acagttcagc gacaagttta tccacgatgt cgctgttagc agcttcatcc
     8761 acggaacgtt cgatgatctt ctcggcgcca gcaacagcca ggatagcaac ttgcttacgc
     8821 agctcttcac gggcacgttt acgctcggct tcaatttctg cctgcgcctg ggccacgatt
     8881 ttagtacgtt cctgttctgc ctcagctttc gcttcgtcca gaatctgcga gcggcgtttg
     8941 ttcgcctgct cgatgattac ctgggcttcc gctttcgctt ttttcagctg gtcggtcgcg
     9001 ctggcctttg caaggtcaag gtccttatgt gctcgttctg cggaagcaag gccgtcagca
     9061 atttcttttt gacgtttttc gatggctgcc attaatggcg gccatacgta cttcatgcag
     9121 aacagaacga acaggacaaa cgcgatggcc tggccgagga ttgttgcgtt aagattcaca
     9181 gcacaatgcc tctatttagt taacgttctg atattgctct ttaaataaaa gcaacgctta
     9241 ctacgcgaca gcgaacatca cgtacagacc cagacctaca gcgatcatcg ggatagcatc
     9301 caccagaccc ataacgataa agaactgagt acgcagcaga ggaatcagat caggttgacg
     9361 cgctgcgcct tccaggaatt tacccccgag gatgccgata ccgatcgcag caccgattgc
     9421 cgccagaccc atcatcacag cggcagccat gtacagcaga tccatattca ggttttccat
     9481 gacagtctcc agtttgtttc agttaaaacg tagtagtgtt ggtaaattaa tgttcttcag
     9541 acgccatcga cagatagacg atcgtcagaa ccatgaagat gaaggcttgc agcgtaatga
     9601 tcaggatgtg gaaaatggcc cacggcacat tcaggatcca ctgtgaccac cacggcaaca
     9661 gaccagcaat cagaatgaaa atcagctcac cggcatacat gttaccgaac agtcgcaaac
     9721 caagtgaaac tggtttggac agcaggctta ccccttcaag gattaagttg acaggaatga
     9781 acgcccagtg attgaacggc tgcagcgtca actctttcgt gaagccgccg atgcctttca
     9841 ttttgatgct gtagaacaga atcaggataa atacgcccaa tgccatagac agcgttacgt
     9901 tcacgtccgc agacggaacc acacgcagtg caggcagacc cagtacatgt tcagcaatgt
     9961 acggcagcag gtcgataggc agtaaatcca tcaggttcat caggaatacc cagacgaaga
    10021 tcgtcagggc cagcggagca atcagcttgc ttttgccatg gtacatgtct ttcacgctac
    10081 cattaacaaa gccgatcacc agctcaatcg cggtctgaaa cttacctggc acaccgctgg
    10141 tcgccttttt ggctacgcta cggaataaaa ccaggaacaa cagacccagc accaccgaga
    10201 agaacatgga gtcaatattg attgtccaga aggtggctgg ggggttttgt ggatccacca
    10261 gcgagaatgt acgcaggtcc agctgaaggt tattcaggtg gtgtcctatg taatcctgcg
    10321 gcgtcatatt ttctgaagcc atgatgcctt ttaccctttg ttgttaatta cagccggtgc
    10381 cagtatctga accaccagca ccaaaaccca cgtaacgatc agcggcaaga ataccgcctt
    10441 taaaaccgcc aacgccacca ccagtaacac caacatcgcc agaactttga aagcttcgcc
    10501 aaatgcgaat gtccaggcca cccggccttt cgctggtgta tgcgcctggt gacgccaggc
    10561 aaatatcata aacaaaacgt taggcagaaa gactgccagg cccccgctta ttgcagagac
    10621 gccccagaag gggtctttga ggctgaacag caatccactt gctatcacca ccagtaactg
    10681 aacgagcaga agcttccgag caacgtttcg actcacgagc gacacagaca tcacgttttt
    10741 cactcctgct cccttcgagg tatgccgcgt gtcgtataaa actttcttta aggcttagag
    10801 tcaagcatca aaaagcggtc aaattatacg gtgcgccccc gtgatttcaa acaataagta
    10861 gccaaaaggt gaataaatgt ttaaatattt ttccagtgca tacaattgcg acttttctgc
    10921 taaccctgtt cgatcatgaa aaactgtaaa taacgcgtaa acactggtga taaagcgtgc
    10981 ttcagatcac atattgcgca tgttcgcgca cagcatattt atttacttgg caaatgatgc
    11041 ctttgcaagt ttatgatatt tcagtctaaa aacagatact gttttaataa atgacattta
    11101 cacaacaaaa acca
//
LOCUS       DAZGLJ010000149        10952 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000149 DAZGLJ010000000
VERSION     DAZGLJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.149"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(60..656)
                     /gene="nrfG"
                     /locus_tag="ACHK49_003440"
     CDS             complement(60..656)
                     /gene="nrfG"
                     /locus_tag="ACHK49_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO9264030.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(653..1036)
                     /gene="nrfF"
                     /locus_tag="ACHK49_003441"
     CDS             complement(653..1036)
                     /gene="nrfF"
                     /locus_tag="ACHK49_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO9264031.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(1029..2747)
                     /gene="nrfE"
                     /locus_tag="ACHK49_003442"
     CDS             complement(1029..2747)
                     /gene="nrfE"
                     /locus_tag="ACHK49_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO9264032.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(2767..3723)
                     /gene="nrfD"
                     /locus_tag="ACHK49_003443"
     CDS             complement(2767..3723)
                     /gene="nrfD"
                     /locus_tag="ACHK49_003443"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO9264033.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(3720..4391)
                     /gene="nrfC"
                     /locus_tag="ACHK49_003444"
     CDS             complement(3720..4391)
                     /gene="nrfC"
                     /locus_tag="ACHK49_003444"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO9264034.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(4388..4954)
                     /gene="nrfB"
                     /locus_tag="ACHK49_003445"
     CDS             complement(4388..4954)
                     /gene="nrfB"
                     /locus_tag="ACHK49_003445"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO9264035.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(4999..6435)
                     /gene="nrfA"
                     /locus_tag="ACHK49_003446"
     CDS             complement(4999..6435)
                     /gene="nrfA"
                     /locus_tag="ACHK49_003446"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO9264036.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6827..8785
                     /gene="acs"
                     /locus_tag="ACHK49_003447"
     CDS             6827..8785
                     /gene="acs"
                     /locus_tag="ACHK49_003447"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO9264037.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            8986..9300
                     /gene="yjcH"
                     /locus_tag="ACHK49_003448"
     CDS             8986..9300
                     /gene="yjcH"
                     /locus_tag="ACHK49_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO9264038.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            9297..10946
                     /gene="actP"
                     /locus_tag="ACHK49_003449"
     CDS             9297..10946
                     /gene="actP"
                     /locus_tag="ACHK49_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO9264039.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT         2529 a         3065 c         2905 g         2453 t
ORIGIN      
        1 atatgttcat tcgattattt tttatacaac tcaataacat aatcttcatc accagatgat
       61 taatctgatc tccgctgcaa caatttcgcc atattaatcg actcgaccag ctgtgtccgg
      121 ttgatccggg gtgagttgag atccatcact ttttgccata gttcgatggc ttgcgcgtaa
      181 ttcgcctgca taaacgcatc ggaagccagc agcatcagag cggtgatttc gttagagtcc
      241 agcgcgaggg ctttgtcgat cattgcgcga gtctgggcgg tcatatgctg gctggcctgg
      301 taataaagca ccgtcgccag cgccgcatac agttcagcgt tctcaccacg tagttgcaac
      361 gcctgacggt acgccagcag cgaattgctg taatcgtttt gccacagata gtactcgccc
      421 agtaacgccc attgttcgct gttttgtgga ttagcgcgga ttttatcctg caatgcctga
      481 agctgcgctt ccggggtttg ctggctggca aactgatgta gcggatcgcg ctgacgctga
      541 tattccgcat gtaccgcctg ccattttgga cttaacagat aactgccgac acagagaaat
      601 accatcaaaa tcgtgagtgt tgtcagcatt ttcaccggta ttttgggctc tttcatcgct
      661 ttgccctcac tcgccagagg atcagtgcca tcagcagtaa caacaccact ggcagcgccc
      721 acaacaccag cgtctgaccc gttaacggtg ggttatagcg aacaaaatct ccgtagcgtt
      781 cggtcatcca gccaatgatt tcgacttcgt tcttcccctc cgccaccatg ctgtaaacct
      841 gatggcgcat actgactgcc accggcgcgt tggattccag taagttttga ttctggcact
      901 gcggacaacg taactggctg gcaatattta acgcctgttg ctgttgttgc ggattggcga
      961 attgccaggt gtctacgacc tgagcgcgcg caaaacaggt aaataacagc agtagcgtga
     1021 gaagtccttt attcatcgcg cttcctcccc cgccatccgc ttaacaatgc gcccgcaatc
     1081 atcaacaatc ctccccccca gatccagcgc acaccgcttt gtacatacaa gcgaaaagcg
     1141 taacgatccg ctccggtttt ttcacccatg accgcatacc agtcatggat gccgttccag
     1201 cgaattgacg gttccatcat ttgctgacga cgtgcttcgt aaaaacgccg ctccggcatc
     1261 agttcaccaa tgcgttgctg atggtcaaac agtgccacta tcgctttttc gctggtgtaa
     1321 ttgcctttgg cttgcagatc gaggcgctca aaacggaagg tgtatcctgc cagcgtcacc
     1381 tgctgacccg gctgtaaatt caggctgatc tcctgacggc tgacgctgga aaccacgatc
     1441 cccgcggcaa ataacagcac gcccgcatga gctaccagcg ccggaagctg cacgcgtttg
     1501 ccagagacaa acgtcgccag cacaatcacc accagcatca acaggccaaa cggtaacgtc
     1561 gcgcggttaa aatacggcgc gccaacggag aggcgtcccc agcccagcag gccgtaaatc
     1621 atcggataaa gcgttcccac cagcacgatc agtagcactg cgcaaaacag caacagcgta
     1681 gcgaggatta acatttcccg cgataacccc gaaaaacgca ccaccgcacc accgtcccgc
     1741 gctcgccagc catacagagc cagagacgca aggctaatca gtgcaaacag gctgaacaac
     1801 ggcacggcgc ggacgttatc cagtgcgaac gcatgaaccg aaaccagaat gccagaacgg
     1861 acaattaagg tacccagtag cgacagcatc agagttacta tcgccagcaa cagcgaccag
     1921 tggcggaaaa tctcccgctg gcgtgtcagg gacaaactgt gcagcagcgc agtggcagaa
     1981 agccagggta ataaagaggc gttttccacc ggatcccaga accaccagcc gccccagccc
     2041 agttcgcaat aggcccacca ggaaccgagg atgatccccg ccgttaatgc gctccagcca
     2101 ggtagtgccc agcgccaaca aattctggcg caggcaccat caaactcgcc gcgcagtaaa
     2161 ctcgccagcg ccacgctcgc cgctaccatc aaaccgccat agccaaggta aagcagcggt
     2221 ggatgaaaga taagaccggg atgttgcagc atcggattga gatcgcggcc ttcgattgct
     2281 ggtggaaata tccgcacgaa gggatcggac cacagcacca caaacagtag cagtgcggcg
     2341 agcatgagag ataaaacggc taacgtcagc ggaaatagcg gatcggtttg ctgccgataa
     2401 tgccaggcaa acagcgcgct ccaggcggaa agcagcagca cccaaagcag cagcgaacct
     2461 tcatggccgc cccacaccgc tgccagcttg agttcccacg acagcaggct atagctatgt
     2521 tgcgcgacat aaatgaccga gaaatcgctg atgagaaaac aatacgtcag tacgccaaag
     2581 gcgagcagca ggagcgcgaa ctgcgccaga atgccgatgc aagtgagtcg catcatggca
     2641 ggccagcgca accgcactcc cgcaaaggcc gtcaacgggg tcaacacgtt gaccccgaga
     2701 cttaacaaca acgccagaaa accgacttca ggaaggaata cgtccagaaa ccacctcttt
     2761 tctggcttac gccaccgtca actgtccggc gtagagaatg aaaaagcgca gcatcaacac
     2821 gccgaccaga ctggccccac acgccgccag cacggcagga atgccggaac tgcgattgac
     2881 ccacggtttg agcaacattg gcacaatcag ccccagcccg gcgacaccaa gccagaacca
     2941 ccacgtccag aaaccgccac ctaatgccgc caccagcgca cgcactttac cgtcatcgcc
     3001 cagcgccaga ccgacaaaaa atgccaccag caggaagatt tcaccccata ccacggggat
     3061 ttccatacgg tgtacaaact gcgcttccgt ggaatgcggg ttactgcgtt ggcgtatcgc
     3121 catggcgatc agcgccaccg ccgcaccgga cgagatgccg gagaagagga acagcaccgg
     3181 caggatcggg ttattgagga acggatacga tttcagcgcc gacagcagaa agccggtata
     3241 agcccccaac aacaccgcca acaccagcat caatgtttcc agtccgcgat gaacgggcgt
     3301 cagcaggctc agaacctttt gcacgatccc cagcttcggc aaccagcgtt gttgcagggc
     3361 aagcaaatca tgttcaaaga ttttcgccag ccacagcacc agcaccacca tgtagagctg
     3421 aaacagcatc acccccatcg acatcaccga ggtaaaactg tagtggaaca tcagcttcca
     3481 gaaggtccac ggtcttgtca ggtggaagac gaggatcaac agaccgagga tcaccgcgcc
     3541 cggcccgaca atcagcgtgg tgcgcagcaa cgtactgtct gcaccgcccg cctgcgggta
     3601 gaagcgacgt aacagcacgg ccagcgtcac cagaccggca gaaataccaa tcaaaaacag
     3661 gtagatggca atcggccagt cccacaccag cgattcaaaa tgaaatgcgg aagtctgcgt
     3721 cattggctca cctccccgta tttaaacggt acgcggtaca gcttcggttt ggttcccagc
     3781 gccagcttgt agcggtaagt aggcttctgg cgcagcagtt gcgaaatctc actgttagga
     3841 tcgtccagat tgccaaacgt cagcgccttc gtcgggcaag cctcaacgca cgcgggcagc
     3901 ttaccggcct gcaaattggt tttacggcag aaatcgcatt tgtccgccgt cttcgtgacc
     3961 ggatggataa agcgcacgcg gtacggacag gcggcgatgc agtactgaca accgacgcag
     4021 agatcaggat taacatcgac aatcccgcta gcggcatcac gaaaagacgc accggtcggg
     4081 cagacgtcaa cgcacggcgc atgatcgcag tgctggcaag acttacggaa gaagcgatat
     4141 ttcacatcag gaaattcgcc ctgcggctcg ctacgaataa tcgtcaggcg cgagacgcct
     4201 tccggcacct tgttcacttc ccgacaagca tccatacagg cggtacagcc gatgcataac
     4261 gactcgtcat gcaccatgcc gtaacgcacc ccattgatat tcaacgtttt cgccaccacg
     4321 cgccctgcgg tgccgctgac ggctgccagc acgccgacgc cggtgagaaa ctggcgacga
     4381 gaccaggtca tggctgctcc ttaagcaacg gaacagacgc cgggttaaag ttcggattgg
     4441 tgcgctgatc gctgtggcaa tcgacgcaaa tcttaatccg tcctttgtca cttaacgtct
     4501 gcatcgtatc ttgctgtgga tgcagggaat ggcagctggc gcacgccact ttggttacgt
     4561 ggacatcgtg cggccagaac gctttttgca actgttccgg cagatgacag gacatacaga
     4621 cgctgttctg ctctccaacc ttgtacatcg gctcgttaaa gcgcatcaca tctttcaccc
     4681 cttcgcggtg ttgcggtgat ggctggccgt gacagttggt gcaggtgacc ggcagtttgt
     4741 tattcgggtt gataacggaa gcatgttttc catgcatacc ttcggtatct ggtttatgac
     4801 agtccagaca ggcggcatcc gggttacgct gctgggtaac ttcgtagcga tcatcagatg
     4861 cctgcgccgc aggggtagcg gtaattccgt tcaggctcca caacaagccc gacgccagca
     4921 ccccggcagt taataacgaa cgtaatacgc tcatattcac tccattgtgt tttccgcccc
     4981 ttgcgaggcg gaacggggtt attggcttaa cagaccattt ttacgtgcct gttcttccca
     5041 ctgcgggatc accgttttga tgaagtcctg cttctcggcc ttgatttgtt ccatgttcag
     5101 gccaatggcc tgctgggctt tctctttggt cgagatatcc gggatctgga tttcatgggt
     5161 gatgcctttg gtcgccagca ggcgcgccag tttggtgcgt gcatccgccg ctttatccat
     5221 cgccgtaccg agcatccgta gaccttcttc cggtgcgtgc atatgaatac cgtgggaagc
     5281 gatcgccaga tcccaacgcc actgggcatg acggatatcg tcctgaatcg gcttcatttc
     5341 ggcttccgtc gcgcctgcat ccagcgctgc tttcgcttcg aagtgagcgt gaaccagttg
     5401 atcttcaacc ttgattttca ggtcgttaat cgactgctta cgttccgcga ccactttttg
     5461 caaagcagct ttgtcctggg tatggcagtt cgcacaagtc tgggcgaagt tatcaaacgg
     5521 attaccaatt ttatggtcgg tgtagagttt gccttcggcg ttctgcactt ttggcatatg
     5581 gcagtcgata caggtcacgt tgtttttacc gtgaatgccc gctgtccagg tttcatattc
     5641 cgggtgctgc gctttcagca ttggcgtttt cgacagggag ttagtccagt cagagaaggc
     5701 aattttgtcg taatactgct ccatattttc gactttcatg ccgtcatccc acgggaattt
     5761 aaccgctttg tttttgccgt cgaagtaata ctccacatgg cactgaccgc aaaccatcga
     5821 ttgctggtcg aaacgtccgg ctttctcaaa aggtttacca atggcttcca tcgcgcgagc
     5881 cgcatacgga cgggaaaggg ttaactccgg tttgcctttg gcgaactctg gtgaggcggt
     5941 gttatggcag tcggcacaac ctaagttgtt gacgatttcc gggccgccgc gcgcccactt
     6001 accgtggaag tagccatctt cgccgtcttt ctggatcaga cgcgccacat ccgggctttt
     6061 acaactccag cacgccatcg gtagcggacc atcttcagcg tttttcggcg caccggtacg
     6121 cagggtttca cgcacatcgg tcacagcaaa ggcatgtcca cgcggcttgt tgtaatcgcg
     6181 cgagaaggga taccccgccc acaggatcac cagccgtgga tcttccgcca gggcgtcaac
     6241 acgctctgac tgttccgagg tggctttcca ggagagatat tgatcgggat gctgcggggc
     6301 aaaggtttca ttcttcgctt ccacagttac aggttttgcg ggagctgccg tttgttcagc
     6361 gtgaacagaa gtgaaaaaga aaaaaggaat caataagctg aagatacggc gtgcgtttat
     6421 ttttatcctt gtcatagggg cttcatccga attgcgccat tgttgcaatg gcggttttta
     6481 ttgttttcac gacagtaacc gcacctacac tgtcatgaca ttgctcgccc ctatgtgtaa
     6541 catataacca cactgtgaat gttgtcttta atcaattgta agtgcatgta aaataccact
     6601 ttagagttag tcagtatctt cctctttttc aacagcatgc ataactgcat gttcctcaaa
     6661 gaattaatca acttttgttg gtgaccttca aaaattaccc tgccgtttat ttgcacaatt
     6721 ctacttttgc gtgatctgtc gcccaaatac taaacaaaac tgccaatacc cctacattta
     6781 acgcttatgc cacatattat taacatccta caaggagaac aaaagcatga gccaaattca
     6841 caaacacacc attcctgcca acatcgcaga ccgttgcctg ataaaccctc agcagtacga
     6901 ggcgatgtat caacaatcta ttaacgtacc tgataccttc tggggcgaac agggaaaaat
     6961 tctcgactgg atcaaaccgt accagaaggt gaaaaatacc tcctttgccc ccggtaatgt
     7021 gtccattaaa tggtacgagg acggcacgct gaatctggcg gcaaactgcc ttgaccgcca
     7081 tctgcaagaa aacggcgatc gcaccgccat catctgggaa ggcgacgacg ccagccagag
     7141 caaacatatc agctataaag agctgcaccg cgacgtgtgc cgcttcgcca ataccctgct
     7201 cgagctgggc attaaaaaag gtgatgtggt ggcgatttat atgccgatgg tgccggaagc
     7261 cgcggttgcg atgctggcct gcgcccgcat tggcgcggtg cattcggtga ttttcggcgg
     7321 cttctcgccg gaagccgttg ccgggcgcat tattgattcc aactcacgac tggtgatcac
     7381 ttccgacgaa ggtgtgcgtg ccgggcgcag tattccgctg aagaaaaacg ttgatgacgc
     7441 gctgaaaaac ccgaacgtca ccagcgtaga gcatgtggtg gtactgaagc gtactggcgg
     7501 gaaaattgac tggcaggaag ggcgcgacct gtggtggcac gacctggttg agcaagcgag
     7561 cgatcagcac caggcggaag agatgaacgc cgaagatccg ctgtttattc tctacacctc
     7621 cggttctacc ggtaagccaa aaggtgtgct gcatactacc ggcggttatc tggtgtacgc
     7681 ggcgctgacc tttaaatatg tctttgatta tcatccgggt gatatctact ggtgcaccgc
     7741 cgatgtgggc tgggtgaccg gacacagtta cttgctgtac ggcccgctgg cctgcggtgc
     7801 gaccacgctg atgtttgaag gcgtacccaa ctggccgacg cctgcccgta tggcgcaggt
     7861 ggtggacaag catcaggtca atattctcta taccgcaccc acggcgatcc gcgcgctgat
     7921 ggcggaaggc gataaagcga tcgagggtac cgaccgttcg tcgctgcgca ttctcggttc
     7981 cgtgggcgag ccaattaacc cggaagcgtg ggagtggtac tggaaaaaaa tcggcaacga
     8041 gaaatgtccg gtggtcgata cctggtggca gaccgaaact ggcggtttca tgatcacgcc
     8101 gctgcctggc gctaccgagc tgaaagccgg ttcggcaaca cgtccgttct tcggcgtgca
     8161 accggcgctg gtcgataacg aaggtaaccc gctggaaggc gctaccgaag gcagcctggt
     8221 gatcaccgac tcctggccgg gtcaggcgcg tacgctgttt ggcgatcacg aacgttttga
     8281 acagacctac ttctccacct tcaaaaatat gtatttcagc ggcgacggcg cgcgtcgcga
     8341 tgaagatggc tattactgga tcaccgggcg tgtggacgac gtgctgaacg tctccggtca
     8401 ccgtctgggg acggcagaga ttgagtcggc gctggtggcg catccgaaga ttgccgaagc
     8461 cgccgtagta ggtattccgc acaatattaa aggtcaggcg atctacgcct acgtcacgct
     8521 taatcacggg gaggaaccgt caccagaact gtacgcagaa gtccgcaact gggtgcgtaa
     8581 agagattggc ccgctggcga cgccagacgt gctgcactgg accgactccc tgcctaaaac
     8641 ccgctccggc aaaattatgc gccgtattct gcgcaaaatt gcggcgggcg ataccagcaa
     8701 cctgggcgat acctcgacgc ttgccgatcc tggcgtagtc gagaagctgc ttgaagagaa
     8761 gcaggctatc gcgatgccat cgtaacccac aattgccgga tgcgagtcgg taacggtttg
     8821 taggcctgat aagacgcgac agcgtcgcat caggcattga ttgccggatg cggcgtataa
     8881 cgccttatcc ggcctacatt cggcaagggt tacccgagcg ttaaccttct cccataaggg
     8941 agcgggaatt aaaacaatcc ctacattacc tctggagaat ctgtgatgaa tggtacgatt
     9001 tatcagcgga tagaagacaa tgcgcatttc agggagttag tcgaaaaacg gcaacggttt
     9061 gccaccatcc tgtcgattat tatgctggca gtttatatcg gctttatttt actgatcgcc
     9121 ttcgcgcccg gctggctggg cacgccgctg aatccgaaca ccagcgtcac acgcggtatt
     9181 ccaattggtg ttggagtgat tgtgatctcc tttgttctca ccggtatcta catctggcgg
     9241 gcgaacggcg aattcgaccg tcttaataat gaagtcctgc atgaggtaca agcatcatga
     9301 aaagagttct gacggcgctt gccgccacac tccctttcgc agctaacgcc gcggatgcta
     9361 ttagcggggc cgtagagcgc cagccaacga actggcaggc gattattatg ttcctgattt
     9421 tcgtcgtgtt tacgctcggc attacctact gggcatcaaa acgcgtacgt tctcgtagcg
     9481 actactacac cgcaggcggc aatatcactg gcttccagaa cgggctggcg attgccgggg
     9541 actatatgtc cgccgcctca ttcttgggga tctccgcgct ggtgtttacc tccggctatg
     9601 acggcttaat ttactcgctg ggcttcctgg tgggctggcc gatcattttg ttcctgattg
     9661 ccgaacgtct gcgtaacctg gggcgctaca cctttgccga tgtggcctct taccgtctga
     9721 aacaagggcc gattcgtatt ctttcggcct gtggttctct ggtggtggtg gcgctttacc
     9781 ttatcgccca gatggtgggc gcaggtaaac tgatcgagct gctgtttggc cttaactatc
     9841 acattgcggt ggtgctggtc ggcgtgctga tgatgatgta cgtcctgttc ggcggcatgc
     9901 tggcgaccac ttgggtacag attatcaaag ccgtgctgtt gctgttcggt gccagcttta
     9961 tggcctttat ggtgatgaaa cacgtcggct ttagcttcaa caatctgttc agtgaagcga
    10021 tggcggtaca cccgaaaggt gtcgacatca tgaagccggg cgggctggtg aaagatccga
    10081 tctccgcgct ctctctgggt ctgggactga tgtttggtac ggcgggcttg ccgcacattc
    10141 tgatgcgctt ctttacagtc agcgatgccc gcgaagcacg taagagcgtg ttctacgcca
    10201 ccgggtttat gggctacttc tatattctga cctttattat cggcttcggc gcgatcatgc
    10261 tggttggtgc gaatccggaa tataaagacg cggcgggcca tctgattggt ggtaacaaca
    10321 tggcggccgt tcacctggcg aatgcagtgg gcggcaacct gttcctcggt tttatttcag
    10381 cggttgcttt tgccaccatt cttgcggtgg ttgctggtct gacgctggcg ggcgcatcag
    10441 cggtttcgca tgacctgtac gctaacgtct tcaaaaaagg cgcgaccgaa cgtgaagaac
    10501 tgcgggtatc gaaaatcacc gtgctgattt taggtgtgat tgccattatc ctcggcgtgc
    10561 tgtttgagaa tcagaacatc gccttcatgg taggcctggc gtttgccatc gcagcgagct
    10621 gtaacttccc gatcattctg ctttctatgt actggtcgaa actgaccact cgtggcgcga
    10681 tgatgggtgg ctggctgggg ctgattaccg cagtggtact gatgatcctc ggcccgacga
    10741 tttgggtaca gatccttggt cacgaaaaag ccatcttccc gtatgaatac ccggcgctgt
    10801 tctctatcag cgtggcattc ctcggcatct ggttcttctc ggcaaccgat aactcagcgg
    10861 aaggtgcgcg cgagcgtgaa ctgttccgtg cacagtttat ccgctcccag accggctttg
    10921 gcgttgagca aggccgcgca cattaatctc tc
//
LOCUS       DAZGLJ010000150        10940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000150 DAZGLJ010000000
VERSION     DAZGLJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.150"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..281)
                     /gene="ihfA"
                     /locus_tag="ACHK49_003450"
     CDS             complement(<1..281)
                     /gene="ihfA"
                     /locus_tag="ACHK49_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO9264040.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENA"
     gene            complement(286..2673)
                     /gene="pheT"
                     /locus_tag="ACHK49_003451"
     CDS             complement(286..2673)
                     /gene="pheT"
                     /locus_tag="ACHK49_003451"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO9264041.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(2688..3671)
                     /gene="pheS"
                     /locus_tag="ACHK49_003452"
     CDS             complement(2688..3671)
                     /gene="pheS"
                     /locus_tag="ACHK49_003452"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO9264042.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(3881..4005)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(3955..3999)
                     /gene="pheM"
                     /locus_tag="ACHK49_003453"
     CDS             complement(3955..3999)
                     /gene="pheM"
                     /locus_tag="ACHK49_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO9264043.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(4122..4478)
                     /gene="rplT"
                     /locus_tag="ACHK49_003454"
     CDS             complement(4122..4478)
                     /gene="rplT"
                     /locus_tag="ACHK49_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO9264044.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(4531..4728)
                     /gene="rpmI"
                     /locus_tag="ACHK49_003455"
     CDS             complement(4531..4728)
                     /gene="rpmI"
                     /locus_tag="ACHK49_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO9264045.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(4825..5367)
                     /gene="infC"
                     /locus_tag="ACHK49_003456"
     CDS             complement(4825..5367)
                     /gene="infC"
                     /locus_tag="ACHK49_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO9264046.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(5371..7299)
                     /gene="thrS"
                     /locus_tag="ACHK49_003457"
     CDS             complement(5371..7299)
                     /gene="thrS"
                     /locus_tag="ACHK49_003457"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO9264047.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            7823..9722
                     /gene="espL1"
                     /locus_tag="ACHK49_003458"
                     /pseudo
     CDS             7823..9722
                     /gene="espL1"
                     /locus_tag="ACHK49_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            9894..10001
                     /gene="yniD"
                     /locus_tag="ACHK49_003459"
     CDS             9894..10001
                     /gene="yniD"
                     /locus_tag="ACHK49_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO9264048.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(10054..10812)
                     /gene="ydiY"
                     /locus_tag="ACHK49_003460"
     CDS             complement(10054..10812)
                     /gene="ydiY"
                     /locus_tag="ACHK49_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO9264049.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGHQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT         2823 a         2792 c         2447 g         2878 t
ORIGIN      
        1 gcgttttcga cccggctttt taacttctgc ccgggtctga aggtcaccac gcgccgtgct
       61 gtaatgggaa tatcctcgcc cgttttcggg ttacgtcccg ggcgttgatt cttatcacgc
      121 agatcgaagt taccaaaacc agagagtttc acctgttcgc cgttttccag agcgcgacgg
      181 atctcttcga aaaacagttc aaccagttct ttggcatccc gcttgctaag cccaagctta
      241 tcaaacagat attctgacat ttcagctttt gtaagcgcca taggttcaat ccctcaatga
      301 tgcctggaat cgctctttta atgcctctac acatttggcg acggtagcgg caatctcctc
      361 ttcttcgagt gtacggctgg tatcttgcag gatcaggctt atggcgaggc tcttataccc
      421 ctccgcaaca cccttaccgc ggtacacgtc aaataagttt acgccaacta cctgatttac
      481 gccaactttc ttacattcgg ataaaatatc cgctgcggga acgttttctg cgaccaccac
      541 cgcgatgtca cgacggttcg ccgggaagcg agaaatctcg cgcgcctgag gcaccacgcg
      601 gtctgcgagc ttgttccact ccagttcgaa caccagagtg cgaccgttaa gatccagttt
      661 acgttccagt tcaggatgaa caaccccaac aaaaccaata cgttcacctt tcagataaat
      721 cgctgcggat tgccccggat gcagtgccgg attcgcttct gcacggaact caacctcatt
      781 cagtttaccg gtcaggtcga gaacggattc aagatcgcct ttcaaatcat agaaatcaac
      841 ggtctctttt gccaggttcc agtgctcttc gtaacggtta ccgcaaatca caccggctaa
      901 catcagatcc tgacgaatgc ccaacggtgc ctgagtatct ggtacgaaac gcagaccgct
      961 ttcgaaaatg cgcacacggt tctgctgacg gttctggttg tacaccacgg ttgccagcag
     1021 gccggtccac agagaaagac gcattgctga catttcaaca gagatcgggc ttggcagcag
     1081 taaggcttca acgcctggat ggatcatctg ctgcactttc ggatcaacga agctgtaggt
     1141 gatcacttcc tgatagcctt tgtcgttgag cagcgttttc acgcgcttga gcgacaggtc
     1201 agcttcacgg tgagtaccca taatcaggct tgcctgtacc ggctcatccg ggatgttgtt
     1261 gtagccgtaa acacgcgcga cttcttcgac cagatcttct tcaatctcca tatcgaaacg
     1321 ccagctcggc gcaactgcct gccactcgtc tttgccttcg gtcacttcgc agccgagacg
     1381 acgcagaatg tcagttacct gctcatccgc aatatgatgg ccgatcaggc gatccagttt
     1441 gctacgacgt aaagtgatgg ttgcacgctt cggcagcgtt gcttcgttgg tgatatcaat
     1501 taccggacca gcctcaccac cgcagatgtc gatcagcaga cgggtcgcac gttccatcgc
     1561 tttgtgctgc agtgccggat caacgccacg ctcataacgg tgagacgcat cagtatgcag
     1621 gccatgacga cgagcacgac cggtgataga cagcgggctg aagaaagcgc attccagcaa
     1681 gacgttttgc gtttcgtcat tcacgccaga gtgttcgcca ccaaagatgc cgcccatcgc
     1741 cagcgccttg ttgtggtcgg cgatgaccag agtgtcagca ttcagcttcg cttcagtgcc
     1801 atcaagcagt accaacgttt cgccctcttt cgccatccgc accacaatgc cgccttcaat
     1861 gcgatcttta tcgaaagcgt gcatcggctg gcccagttcg agcagtacat agttggtgac
     1921 gtcaacaact gcatcgatag aacggatccc gcaacgacgc agtttttctt tcatccacag
     1981 cggagttggc gctttaacgt taatgccttt taccacacgg ccaagataac gcgggcaggc
     2041 ttccggcgct tcgactgtaa tcggcagcgt gtcgtcgatg gtcgcaccaa ccggaacgat
     2101 ttccggttca accagcggca gctgattcag cacggcaacg tcacgcgcaa caccaatgat
     2161 acctaaacag tcggcacggt ttggcgtcac gctgatttcg atggtgttgt catcaagttt
     2221 caggtattca cggatgtcgg tgccaatcgg cgcatccgca ggcagctcga taatgccgct
     2281 atgatcgtca gaaattccca gctcagaaaa ggagcacagc atcccttcag acggttcacc
     2341 acgcagtttc gccgctttaa ttttgaaatc acccggcaga acagcaccaa tggtagctac
     2401 cgccacacgc aggccctgac ggcagtttgg cgcaccgcag acgatgtcca gcaggcgatc
     2461 gccgccaaca ttcacttttg tcacacgcag tttgtcagcg ttcggatgct gcgcacactc
     2521 aaccacttca ccaacgacca cgccgtggaa gctaccggca accggttcta caccgtcaac
     2581 ttccaggccc gccatagtga tttgattcgc cagcgcatcg ctatcaatcg ccgggttcac
     2641 ccattcgcgt aaccacagtt cactgaattt cataatctat tcctgcctta tttaaactgt
     2701 ttgaggaaac gcagatcatt ttcgaagaat gaacgcaggt cggtgacgcc gtaacgcaac
     2761 atagtcagac gctccatccc catcccgaag gcgaaaccag agtaaacttc cgggtcgatg
     2821 ccaacgttac gcagcacgtt cggatgcacc atcccgcaac ccagcacttc cagccattta
     2881 ccgtttttac ccatgacgtc aacttctgca gaaggctcgg tgaacgggaa gtaggaagga
     2941 cggaagcgaa tctgcaaatc ttcctcaaag aagttacgca ggaaatcgtg cagagtgcct
     3001 ttcaggttgg taaagctgat gttggtatca acaatcagac cttccatctg atggaacatc
     3061 ggcgtgtgag tctggtcgta gtcgttacga taaacgcggc caggcgcaat gatacgaatc
     3121 ggtggctgct gggctttcat ggtgcggatc tgtacgccag aggtctgggt acgcagcagg
     3181 cgggtagtgt caaaccagaa agtgtcgtgg tcagcgcgcg ccgggtggtg accaggaatg
     3241 ttcagagcat cgaagttatg ataatcgtct tcgatttccg gcccggttgc cacggtaaag
     3301 ccaagctcac cgaagaaact ttcgatacgg tcgatagtac gggtaaccgg atgcagaccg
     3361 ccgttttcaa tgcgacgacc tggcagagag acatcaatcg tttccgccgc cagacgcgca
     3421 ttcagtgcag cgctttccag ttccgcttta cgcgcattca gcgcctgctg aacctgctct
     3481 ttcgcttcgt tgataaccgc accagctgcc ggacgctctt ctggcggcag ctcacgcagg
     3541 gtcgtcatct gaagggttaa gtgccctttt ttacccaaat attcgacgcg cacattatct
     3601 aacgcggcaa catctgacgc ctggctaatg gccgccttcg cactggcaac cagttctgcg
     3661 agatgtgaca tggttttcct cattgtgtca gtggtgacac tggttcgttg gacttagagc
     3721 ctatcccatc aggctatttt acttgccatt ttggtcccgg gcagtgctcg aaattctcac
     3781 gtacttaaat acgctccggt ttctccgcgc tggccgtgtc cagactggct gcgacaatta
     3841 cacctgatga gacaggcttt ttatttttca aaacgcgcat acaaaaaaag cctccactgg
     3901 gaggctttca ggcgctgttt tccgtttctc ttctcacgcg ctagcctcct ggattcaggt
     3961 gctaaagtaa aaaaagaagc ggaaaatagc agcattcatt gcttgcgtta ccttttggta
     4021 ctcttcaaaa gacctttatt gaaaaggcta cggcgataaa agtcaatgtt ttgatggcgt
     4081 tgaaacgaaa agagggagac tagctccctc tttcaactgg cttatgccag agctgctttc
     4141 gctttttcaa ccagagcggt gaacgctact ttgtcgaata ctgcgatatc agccaggatc
     4201 ttacggtcga tttcaacaga ggcttttttc aggccattga tgaatttgct gtaagaaata
     4261 ccgttctgac gtgctgctgc gttgatacgc gcaatccaca gttgacggaa ctgacgctta
     4321 cgttgacgac ggtcacggta agcatactga ccagctttga taacagcctg gaaggcaacg
     4381 cggtatacgc gagaacgcgc accgtagtag cctttagctt gtttcaaaat tttcttgtga
     4441 cgtgcgcgtg caataacacc acgttttacg cgagccatat gtgctctcct gtatctatat
     4501 tctaattaaa aagttaaaaa cgttaacggc ttatgcgtac ggcaggcacg cgattaccag
     4561 gcccagatcg cctttggaaa ccatggcttt cggacgcagg tgacgtttac gtttggtcgc
     4621 ttttttggtc agaatgtgac gcaggttagc gtgcttgtgc ttaaaaccac ctttaccggt
     4681 ttttttgaag cgcttagcag caccgcgtac ggtcttaatt tttggcattt taataacttc
     4741 cacttcgcat tgttaataaa cgaaacaaag gcgaacaaag cctgtgaagc ccgaaggctc
     4801 cacagacagt gctacttgaa ggccttactg tttcttctta ggagcgagca ccatgatcat
     4861 ctgacggcct tcgatcttcg ttgggaagga ttcgaccact gccagttctt gcaaatcgtc
     4921 tttcacgcga ttaagcactt ccataccgat ttgctggtgc gccatctcac gaccgcggaa
     4981 acgcagcgtg attttggctt tatcaccctc ttcgagaaag cgaatcaggc tgcggagttt
     5041 tacctgatag tcgccttcat ctgtaccagg acggaattta atttccttaa cctggataac
     5101 tttttgcttt ttcttctgtt ccttagaaga cttgctcttt tcatagagga atttgccgta
     5161 atccattata cgacaaaccg gcggctcggc gttagggctg atctcgacta agtctactcc
     5221 ggcttcttct gctttctcca gagcttctct cagactcaca ataccaagct gctcgccttc
     5281 cagacctgtt aagcgaactt cctgggcgcg aatttcgcca ttgatacggt tagggcgcgc
     5341 cgtttgaact cgttttccgc ctttaatacc ttattcctcc aattgtttaa gactgcggct
     5401 gcgaatctct tgttgcagct tctcgatcac ttcatttacg tccatgcttc ccaggtcttt
     5461 accacggcgg gtgcgaacgg caactttgcc tgattccacc tctttatcac cacagaccag
     5521 catatatggg acgcgacgca aagtgtgctc gcggatttta aagccaatct tctcatttct
     5581 caagtctgct ttaacacgaa tgcccgcatt tgatagtttt tgcgtcaatt cgttaacgta
     5641 ttcagactgt gaatcggtaa tattcatgat aacaacctga actggcgcaa gccaggtcgg
     5701 gaagaaacca gcgaattctt cggtcaggat accgatgaaa cgttccatcg atcccagaat
     5761 tgcgcggtga atcattaccg gtactttacg ttcattgtct tcgcctacat aagaagcgct
     5821 cagacgagaa ggcaaagaga agtccagctg tactgtaccg cactgccatg cacgatcgag
     5881 gcagtcatac agggtaaatt caattttcgg accgtagaaa gcgccttcac ccagttgata
     5941 ttcaaacggg atgttgtttt cttccagcgc aaccgccagg tccgcctcag cacgatccca
     6001 catttcatca ctgccaatac gtttttcagg acgagtggag agtttgacga cgatcttctc
     6061 gaagccaaaa gtgctgtaca tatcatagac taaacggata catccgttaa cttcatcgcg
     6121 aatttgttct tcagtacaga agatatgcgc gtcatcctgg gtaaatccac gcacgcgcat
     6181 caggccatgc agcgaacctg acggctcatt acggtggcag ctaccaaact cggccatacg
     6241 cagcggcaga tcgcgataag acttcagccc ctggttgaaa atttgtacgt gacccgggca
     6301 gttcatcggc ttaatgcagt attcacggtt ctcagaagac gtggtgaaca ttgcatcttt
     6361 gtagttgtcc cagtgaccgg ttttttccca caggacacgg tccatcatga acggaccttt
     6421 aacttcctga tactggtact ctttcagttt agaacgaaca aacacttcta gttcacggaa
     6481 gatggtccag ccgtcgttgt gccagaatac catacccggc gcttcttcct gcatatggta
     6541 caggtcgagc tgtttaccga ttttacggtg gtcgcgtttc gcggcttctt ccaggcgctg
     6601 caggtaagcg ttaagcgctt ttttgtctgc ccacgccgta ccgtaaatac gttgcaacat
     6661 tttgttgttg ctgtcgccac gccagtaagc ccctgccgtt ttcattagtt tgaaatgatg
     6721 gcagaaacgc atgttcggta cgtgcggacc gcggcacata tcgacatatt cttcatggaa
     6781 gtacagacct ggcttgtcat catgggcgat gttttcgtca agaatggaga ctttgtagct
     6841 ctccccacgg ttggcgaaag tttcacgcgc ttcgtgccag ctgactttct tcttaatgac
     6901 gtcgtagttt ttctcagcaa gctcatgcat ccgcttctcg agtgcttcga catcttcctg
     6961 ggttaacgtg cggtcaagat caacgtcgta ataaaaaccg ttgtcaataa ccgggccgat
     7021 tgccattttg gtatgcggcc aaagttgttt aatcgcgtgc cctaacaggt gcgcacagga
     7081 gtgacgaatg atctccagac cgtcttcgtc tttggcggta atgatggaca gttgtgcgtc
     7141 gttttcaatc agatcgcaag catcaaccag ttcgccatta acgcgccctg cgatacaggc
     7201 tttcgccaga cctggaccaa tgtccagcgc aacatccatg gggcttacag cgtggtcgta
     7261 atggcgttgg ctgccatcag gaagagttat aacaggcatt ttatatcctt atttgcagtg
     7321 gtgacccaca cgaaagatca catacaaaga aaaatttgtt tattaacagt tcattgcgaa
     7381 accatctagc caacaaatgc taaattggtt cgcaatcagg tacacaaatg aaagacatta
     7441 acctctcacc ttccacccgg ttgagcgatg ttaacactaa aaaaagggag attgtacctt
     7501 tccgtttcac attgattcga ttcgcaattc gtttgtagtg agtacaccag caaaacgctt
     7561 aaagaaccac tgataacatc acggtgcgtc tgaaaggttt actgcgagcg gatttacagc
     7621 agcataaact gaatccagca aagttcgttg cgtgatttgg cggtagtatt ttaaacgctc
     7681 atgagattaa tgacgaagtg gtcatatcac aatgataaaa gtgacacaat tcttataaca
     7741 atttttcgtg cacatttcgt tctggcgata ataattaatc atcatcctca aaacctccag
     7801 atatatataa ggcgaataga ttatgagtca aaacgatatc attatcagaa ctcattataa
     7861 gtctcctcat agattgcaca tcgatagcga cataccaaca ccttcatcag agcctattaa
     7921 taaatttgcg cgccagctca tcaccctact tgatacctct gacttaagtt cgatgctgtc
     7981 atactgtgtt actcaggaat ttaccgcaaa ctgtcgaaaa atatcacaaa attgttattc
     8041 cactgccctt tttaccatta accttgccac ttcacccatc catgcagaaa atatactcat
     8101 tacattacac tataaaaaag atatcatttc cttattactg gaaaccacgc ctattaaagc
     8161 taaccatttg cgaagcatac tggattatat tgaacaggaa cagttaactg ctgaagatcg
     8221 taaccattgt atgaaactgt ctaaaaaaat ccatagagaa aaaactatac acccaacagt
     8281 aaatctcaat ggtagtgcat gttttttgca atctccttct gacgctattt tttgtcgcca
     8341 tctgtcattg caatacgccc ttgattcatt gagaaatgga aaaggcaaag tcaacctgat
     8401 taaacattac tcctccgttg aatccataca gcagcatgtc cccttagtcc gggacgcgga
     8461 gttcagagca ttacttcgcc atcctcctgc agggagtcgc gttatcgcga gtaaggattt
     8521 tggcttcgct ttagattttt tcttttgtcg aatgatggca aacaatatca gtcatatgtc
     8581 cgcgatttta tatatagaca atcatacttt gtcagtaagg ctacgaataa agcagtcagt
     8641 gtatggacaa ttaaactatg ttgtgtccgt ttacgacccg aacgatacca acgttgccgt
     8701 cagagacacc cacaggacag cacggggctt tctttcgctt gataagttca tcagttcagg
     8761 tcccgatgct cagacctggg ctgataggta tgttcgcaac tgtgcaattg ctattctgcc
     8821 cctattacct gtgggagttc caggggctat tttcgcgggt attgcatcac gaatgccatt
     8881 tgcccctata catccatcgg caatgttgtt aataatggct acaggccaga ctcaacagct
     8941 tattacatta ttcaaacagt tgcccatact ccctgaaaaa gaaatcattg aaataataac
     9001 tgcgcagaat agcgttggta cacctgcttt atttttggct atgatgaacg gacatactga
     9061 caacgtgaaa atatttatgc aagaaattca gtcactggta gataatcaca tcattcatga
     9121 agataacctg gttaaattac tgcaaactaa aagtgctaac gaaacacctg gactctatat
     9181 ctccatgttg tatggattcg atgaaataat cgatatcttt ctgaatgcat taaccactcc
     9241 aatagcacaa gaacttttaa acaaaaaaat ggtgatggat attttagcca tgaaaacacg
     9301 tgatggtgaa ccagggttat ttgctgcaat ggaaaataat caccctttgt gtgtcacacg
     9361 gttcctctct aaagtttatg ggatcgccgt taaatataaa ctcagcaaaa tcaacatcat
     9421 ggacttatta aaaggcgcga cagcacaggg aacccctgca ttatacatcg ccatgagcaa
     9481 gggtaatgaa gacgtcgtgt tatcttatat atcgacgctg ggtgcttttg caaaaaaaac
     9541 attcttttag tcaacatcag ttatttacac tattggccgc taaaaatcat gacaacatgt
     9601 cagctgttca tatagccatt catcataagc attataaaac tgtagaaaca tattatgctg
     9661 ctataaatgt catcagccaa agcctgagtt ttagtgctga tgaaataaag acgtatttat
     9721 aaccagctat ttacgatata tatcgataca taattttatt tttttcataa aacgcagaaa
     9781 cagtgaccag gtgtacaacg ccacccaacg gctgtaataa caaaaaatcc cttgatgccc
     9841 gtcccttttg ttacactccg ttatcacgca caagagatat gcaggacact ggtatgccga
     9901 ctaaacgctt tgataaaaaa cactggaaga tggtggtggt gctactggca atctgtggcg
     9961 ctatgttgtt gctacgttgg gcagcaatga tttggggctg agcagtgaca aacgaagaca
    10021 gccagaccgc ataacacggt ccggcagata attttacatg ctgtagccca gcgataaggt
    10081 tgtccgacgg tcggtatgtt ccggtgcaga ctctggaggt tcagagttcc atgtgacgtt
    10141 gtaagccacc ttcagtccaa aatgttcatt aatagcaaca tttaatgcgc tttcggagtt
    10201 cagcgttgtg tcttccgcgc caaagaccga aacaccctgc gtaaatttag cgttgtcagt
    10261 caactgccat gcataagccc cggaggcata gcccagcggc tgggtttcac tggcattgtc
    10321 ggtatatttg tcgtaacgca cacctggacc gaattcaaag cggaaactgt gtaccgggcc
    10381 attcaaaaac tgatgaccat aacccgcggt caacacatcg cgctctcgat agccgttata
    10441 acggtctgtc agccagcttg cctgaccaaa taaatagtca taatcagtta aattaaaacg
    10501 gctacgcccg cccgccgcat atttttctga agaacgctca tcattagaag aagtattact
    10561 ggcgttcccc cacagcgacc aggcagtggt ttgtccatac caggtcatgg tggtgtcagc
    10621 cgtaagtgag gagcttttcg tattgcctga ttgtgcaaga tatcctgcgt tcagattacc
    10681 ttcgaaaggt tttttagcgc tggcaggatc gtccatgaca gtaaaaacgg aatcatcggc
    10741 agctgcattc agtgacgcaa acatgccccc cgccaacata acgatggcag gaactgtctt
    10801 caaaagcttc atttatcaag agtccgtaca acaaaaaaag agaccatcgc ggtcccggaa
    10861 actttcttaa ggatcaaaga ttagcgtccc tggaaaggta acgaattata aaaaggcgcg
    10921 aataacttag caatgtattc
//
LOCUS       DAZGLJ010000151        10907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000151 DAZGLJ010000000
VERSION     DAZGLJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.151"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            63..824
                     /gene="adiY"
                     /locus_tag="ACHK49_003461"
     CDS             63..824
                     /gene="adiY"
                     /locus_tag="ACHK49_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO9264050.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            961..2298
                     /gene="adiC"
                     /locus_tag="ACHK49_003462"
     CDS             961..2298
                     /gene="adiC"
                     /locus_tag="ACHK49_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO9264051.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDVPISKD"
     gene            2402..4045
                     /gene="eptA"
                     /locus_tag="ACHK49_003463"
     CDS             2402..4045
                     /gene="eptA"
                     /locus_tag="ACHK49_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO9264052.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4042..4710
                     /gene="basR"
                     /locus_tag="ACHK49_003464"
     CDS             4042..4710
                     /gene="basR"
                     /locus_tag="ACHK49_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO9264053.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4711..5811
                     /gene="pmrB"
                     /locus_tag="ACHK49_003465"
     CDS             4711..5811
                     /gene="pmrB"
                     /locus_tag="ACHK49_003465"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO9264054.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5787..5876)
                     /gene="pmrR"
                     /locus_tag="ACHK49_003466"
     CDS             complement(5787..5876)
                     /gene="pmrR"
                     /locus_tag="ACHK49_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO9264055.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5988..7490)
                     /gene="proP"
                     /locus_tag="ACHK49_003467"
     CDS             complement(5988..7490)
                     /gene="proP"
                     /locus_tag="ACHK49_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO9264056.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7754..8632)
                     /gene="rdcB"
                     /locus_tag="ACHK49_003468"
     CDS             complement(7754..8632)
                     /gene="rdcB"
                     /locus_tag="ACHK49_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO9264057.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLA"
     gene            complement(8629..10857)
                     /gene="crfC"
                     /locus_tag="ACHK49_003469"
     CDS             complement(8629..10857)
                     /gene="crfC"
                     /locus_tag="ACHK49_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO9264058.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDRLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALASANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGAVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQRTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2721 a         2846 c         2698 g         2642 t
ORIGIN      
        1 tttcccggta tcgctttgct ttagcgagaa ctggtctttt atttgcactc aggagtacat
       61 gtatgaggat ttgcagcgac caaccttgta ttgttttatt gactgaaaaa gatgtctgga
      121 taagggtgaa tgggaaagaa cctattagcc ttaaagctaa ccatatggcg ttattaaatt
      181 gtgaaaataa tattatcgac gtctcctctc ttaacaacac tttggttgct catattagtc
      241 acgacatcat caaagattac ctccggtttc tgaataaaga tctctcgcaa ataccagtat
      301 ggcaacgtag cgctacgccc atactcaccc tgccatgcct gacgccagac gtctttcgcg
      361 ttgccgcgca acacagcatg atgcccgcag aaactgagtc agaaaaggaa cgaacacgcg
      421 cattattatt cacggtgcta tcccgttttc tcgacagtaa aaaattcctt tcattaatga
      481 tgtatatgtt acgtaattgt gtaagtgaca gcgtttatca aattattgaa agcgatattc
      541 acaaagactg gaatcttagt atggtagcca gttgtttatg tcttagccca agtctgttaa
      601 agaaaaagtt gaaaagcgaa aacaccagtt atagccaaat aatcaccacc tgccgcatgc
      661 gttatgccgt aaatgaatta atgatggacg gtaaaaatat ctctcaggta tcacagtcct
      721 gcggctacaa cagtacgtcg tactttattt ctgtctttaa agacttctac ggtatgacgc
      781 cgctgcatta tgttagtcag cacagagaac gcactgtcgc ctgattttta accttaacga
      841 agagctatat taataacggc atcagcgata acccggtcga taataattca actatcgaat
      901 gcaggcgtat gatatgacgt aatttattgt cacgaagctc gccttcgcag gagtttaatt
      961 atgtcttcgg atgctgatgc tcacaaagtg ggcttaatcc ccgtcaccct gatggtgtcg
     1021 gggaatatta tggggtcagg tgtttttctg ttacctgcaa acctggcctc tactggcggg
     1081 attgccattt atggatggtt ggtgacgatt atcggtgcgc tggggctctc gatggtatac
     1141 gccaaaatgt cgttcctcga tccaagtcct ggtggttctt acgcgtacgc ccgccgctgc
     1201 tttggcccgt ttctcggtta tcaaaccaac gtcctctact ggctggcctg ctggatcggc
     1261 aatatcgcca tggtggtcat tggcgtagga tatttaagtt acttcttccc gattctgaaa
     1321 gatccattgg tattaaccat cacctgcgtc gtggtgctgt ggatcttcgt cctgctgaac
     1381 attgtcggtc cgaaaatgat cacccgtgtg caggccgttg ccaccgtgct ggcgctgatc
     1441 cccatcgtcg ggattgccgt atttggctgg ttctggttcc gtggtgaaac ctatatggcg
     1501 gcatggaacg tcagcggcct gggcaccttc ggtgcaattc aaagtaccct taacgttacg
     1561 ctgtggtcgt tcatcggtgt ggaaagtgcc tccgttgccg caggtgtggt gaaaaacccg
     1621 aaacgcaatg tccctatcgc caccattggt ggggtattga ttgccgccgt ttgctatgta
     1681 ctttctacca ccgcgattat ggggatgatc cctaatgccg cactgcgcgt ttctgcttcg
     1741 ccattcggtg atgccgcacg gatggcgttg ggtgacaccg ccggggccat tgtttccttc
     1801 tgcgcagctg cgggttgctt aggttcactg ggcggctgga cgttgctggc gggtcaaacg
     1861 gcgaaagccg ctgccgatga cggactgttc ccaccgattt ttgcccgtgt aaataaagcg
     1921 ggcacgccag tggcggggct gattatcgtc ggtattttga tgaccatctt ccagctcagc
     1981 agcatttcac caaacgcgac caaagagttc ggtctggttt cttccgtctc ggtcatcttt
     2041 acactggtgc catatcttta cacctgtgcg gcgttactgc tgctcggtca cggtcacttt
     2101 ggtaaagcac gcccggcata tctggctgtt actaccattg ccttcctcta ctgcatttgg
     2161 gccgtggtgg ggtccggggc gaaagaggtt atgtggtcat ttgtcaccct gatggtaatc
     2221 accgccatgt atgcgctgaa ttacaaccgg ctgcataaaa acccgtatcc cttagatgta
     2281 ccaataagca aagattaatt ccccttaatc cagcaaacat aaaagccaac cttaagaact
     2341 taaggttggc ttaattttgc tttgcgagca tatgcgcact ttgttcgatg gaaacaccgt
     2401 gatgttgaag cgcctactaa aaagaccctc tttgaattta ctcgcctggc tattgttggc
     2461 cgctttttat atctctatct gcctgaatat tgcctttttt aaacaggtgt tgcaggcgct
     2521 gccgctggat tcgctgcata acgtactggt tttcttgtcg atgccggtcg tcgctttcag
     2581 cgtgattaat attgtcctga cactaagctc tttcttatgg cttaatcgac cactggcctg
     2641 cctgtttatt ctggttggcg cggctgcaca atatttcata atgacttacg gcatcgtcat
     2701 cgaccgctcg atgattgcca atattattga taccactccg gcagaaagtt atgcgctgat
     2761 gacaccgcaa atgttattaa cgctgggatt cagcggcgtg cttgctgcgc tgattgcctg
     2821 ctggataaaa atcaaacctg ccacctcgcg tctgcgcagt gttcttttcc gtggagccaa
     2881 tattctggtt tctgtactac tgattttgct ggtcgccgca ctgttttata aagactacgc
     2941 ctcgttgttc cgcaataaca aagagctggt gaaatcctta agcccctcta acagcattgt
     3001 tgccagctgg tcatggtact cccatcagcg actggcaaat ctaccgctgg tgcgaattgg
     3061 tgaagacgcc caccgcaacc cgttaatgca gaacgaaaaa cgtaaaaatt tgaccatcct
     3121 gattgtcggc gaaacctcgc gggcggagaa cttctccctc aacggctacc cgcgtgaaac
     3181 taacccgcgg ctggcgaaag ataacgtggt ctatttccct aataccgcat cttgcggcac
     3241 ggcaacggca gtttcagtac cgtgcatgtt ctcggatatg ccgcgtgagc attacaaaga
     3301 agagctggca cagcaccagg aaggcgtgct ggatatcatt cagcgagcgg gcatcaacgt
     3361 gctgtggaat gacaacgatg gcggctgtaa aggtgcctgc gaccgcgtgc ctcaccagaa
     3421 cgtcaccgcg ctgaatctgc ctggtcagtg catcaacggc gaatgctatg acgaagtgct
     3481 gttccacggg ctggaagagt acatcaataa cctgcagggt gatggcgtga ttgtcttaca
     3541 caccatcggc agccacggtc cgacctatta caaccgctat ccgccgcagt tcaggaaatt
     3601 taccccaacc tgcgacacca atgagatcca gacctgtagc aaagagcaac tggtgaacac
     3661 ttacgacaac acgctggttt acgtcgacta tattgttgat aaagcgatta atctgctgaa
     3721 agaacatcag gataaattta ccaccagcct ggtttatctt tctgaccacg gtgaatcgtt
     3781 aggtgaaaat ggcatctatc tgcacggtct gccttatgcc atcgccccgg atagccaaaa
     3841 acaggtgccg atgctgctgt ggctgtcgga ggattatcaa aaacggtatc aggttgacca
     3901 gaactgcctg caaaaacagg cgcaaacgca acactattca caagacaatt tattctccac
     3961 gctattggga ttaactggcg ttgagacgaa gtattaccag gctgcggatg atattctgca
     4021 aacttgcagg agagtgagtg aatgaaaatt ctgattgttg aagacgatac gctgttattg
     4081 cagggattga ttctggcggc gcaaactgaa ggctacgcgt gcgatggcgt gacaaccgcg
     4141 cggatggcgg aacaaagcct tgaggccggt cattacagcc tggtggtact ggatttaggg
     4201 ttacccgacg aagatggact gcattttctc gcccgtatcc ggcagaaaaa atacaccctg
     4261 ccagtactga tcctcaccgc tcgcgatacg ctgaccgaca aaatcgctgg gctggatgtc
     4321 ggtgccgatg actatctggt gaagcctttt gcgctggaag agttacacgc ccgtatccgc
     4381 gccctgctac gacgccataa taatcagggc gaaagtgagc tgattgttgg caatctgacg
     4441 ctgaatatgg gtcgccgtca ggtatggatg ggcggtgaag agttgattct gacgcccaaa
     4501 gaatatgctc tgctgtcacg gttaatgctc aaagcaggca gtccggtgca tcgggaaatt
     4561 ctctacaacg acatctataa ctgggacaat gaaccctcga ccaacaccct ggaagtgcat
     4621 atccacaatc tgcgcgacaa agtgggcaaa gcccgtatcc gcaccgtgcg cggctttggc
     4681 tatatgctgg tcgcgaatga ggaaaactaa ttgaatctga tgcattttct gcgccgacca
     4741 atatcgctgc gccaacggct gatattgacc atcggggcca ttttgttggt gtttgagctg
     4801 atcagcgtct tctggctatg gcatgaaagt accgagcaga ttcagctgtt tgagcaggcg
     4861 ctacgcgaca atcgcaacaa cgatcgccac atcatgcgtg agatccgcga ggcggtcgcc
     4921 agcctgattg tccccggcgt ctttatggtc agcctgacgc tatttatctg ctatcaggcg
     4981 gtgcgccgca tcacccgccc gctggcggag ctgcaaaaag agctggaagc gcgcactgcc
     5041 gacaacttaa cgcccattgc cattcacagc gccaccctcg aaatcgaagc ggtggtttcg
     5101 gcgttaaacg atctggtcag tcgcctgacc agcacgctgg ataacgaaag gttgtttacc
     5161 gctgacgtcg cgcacgaact gcgaacgcca ctggcggggg tgcgtttgca tctggaactg
     5221 ctggcgaaaa cgcatcacat tgatgtagca ccgttagtgg cacgacttga tcagatgatg
     5281 gagagcgtct cccagctgct gcaactggcg cgtgccggac agtcattttc ttccggtaat
     5341 tatcaacatg taaaactgct ggaagatgtc attctcccct cgtatgacga actcagtacc
     5401 atgctcgacc agcgacagca aaccctgcta ttgccagaga gcgccgccga catcaccgtt
     5461 cagggtgatg caaccctgct gcggatgtta ttgcgaaacc tggtagaaaa cgcccatcgt
     5521 tacagcccgc aaggcagcaa cattatgatt aagctgcaag aagatggcgg agcggtcatg
     5581 gcagttgaag atgaaggacc aggtattgat gagagtaaat gcggggagtt gagtaaagcg
     5641 tttgtacgta tggacagccg ttatggcggg attggtctgg ggttaagtat tgtcagccgc
     5701 attacacagt tgcatcacgg gcagtttttc ctgcaaaacc ggcaagagac ttccggcacg
     5761 cgggcctggg tacggctgaa gaaagatcag aacgtggcaa accagatata aagaaagctg
     5821 ctgaccacca gcacgctgaa cacggtagtt aaactttcat aaacacggtt tttcattgca
     5881 ctctcctccg ttaacctgga ggagagtatg cgcgtcagag attaagtgaa ccttaagagt
     5941 tcaattggcc tgataagaca gcgtcacatc aggccatccg tttcagctta ttcatcaatt
     6001 cgcggatgtt gctgcaccag gcgggtacgt ttcgcctgca aatcggcaat ctcgtggtca
     6061 atattatcga ttttctgctc gatattatcg taatgctcga cgagaatttc cttcgcttcc
     6121 tgtatatctg acgccgccgg tgtcgcacct ttcaacggac gatttgccgt ctctttcatg
     6181 gttacgccgg tgattaaacc aatcaccgcc actaccatca ggtaataggc aggcatcatc
     6241 agattctgcg agctttcgac cagccaggcc gccagtgttg gcgtcagacc ggcaaccagc
     6301 accgaaatat taaatgccgc cgccagcgcg ctgtaacgga tatgcgtcgg gaacatcgct
     6361 ggcaaggtag aagccataac gcccgtaaag cagttaagga tcaccgccag catcagtaac
     6421 ccggcaaaaa tcaggccgat gacgttactg ttaatcagaa taaacgccgg gatcgccaac
     6481 acaaacaggg caacactacc aagtagcaca aacggacgac ggccaaaacg gtcactcagc
     6541 aagcccatca ccggctggac aaacagcata ccgatcataa tggcgataat aatcagcacc
     6601 ccgtggtctt cggagtaatg caggttatgc gacaaataac tcggcatata ggtcagcaac
     6661 atgtagtaag tcacgttggt ggcaattacc agaccaatac atgtcaacag gctgcgccag
     6721 tgtttggtgg caatctcttt aaacgagact ttcgggccat cctgcaaacc ttcgcggtcg
     6781 ccctgttcca gtttatcgac atgctgctgg aacgccggag tttcttccag cgcatggcgc
     6841 aggtaaagcc cgataatccc taacggcaga gcaataaaga acggaatacg ccagccccag
     6901 tcgaggaagt tcgcttcgcc gacaatggtc gaaattaaca ccactacgcc agcacccagc
     6961 acaaacccgg caatagaacc gaagtccagc cagctgccca taaagccacg tttacggtca
     7021 ggtgagtatt ccgcaacaaa tatcgacgcc ccggtatatt caccgccgac cgagaaacct
     7081 tgtgccatct tacagatcaa cagcagaatc ggtgcccaaa taccaatcgt gtcgtaggac
     7141 ggtattaagc caatacagaa cgtactgatc gacataatca caatagtgat agcgaggatc
     7201 ttctggcgac catatttatc gcccaacata ccaaagaaga gcccgccaag cggtcgaatc
     7261 agaaagggaa cggagaaagt ggcaagtgca gcaaccatct gcacgctggg gtcagccccc
     7321 gggaaaaaaa ctttacctaa tgcgtaagca acaaaaccat aaacaccaaa atcaaaccat
     7381 tccattgcat tacccagtga tgctgcggta atggctttac gcagtttacc gtcatcaata
     7441 atggtgacat cacgaagggt aattggtttt acttttttcc ttttcagcat agctttcctc
     7501 gcagagttgg cccatttccg caaacgccag aaaaatcagc gcgacggccg caaaagggga
     7561 gcgggaattg caggatgcaa tctctgtaac aagcgtagcg ggtttgctta caccctccgg
     7621 ttaaggaatg tacaatcaaa ccagttgacg cctgaattgg gcagttaatg acctttttac
     7681 cctaccagtg tttaaatttg tgatcaactt cacacatgga tttcgtttag gactcattga
     7741 tgtaactgat tatttacgcc aaatttacag agaagatttc ctcgagcatt gcccccgcca
     7801 gacgctgcgc tgaacagagt cgtggcatcc ccagcgcgac agtttgccag cgttgggtca
     7861 ctgaccagca aacgggttgt ttatctgccg tggtccactt tcccagccaa cttaaaaaat
     7921 cagcatgatc cccgcccgtc ggaacggtcg aagaggcaaa ccactgatgg taatagtgac
     7981 gcgtggtcgg ggccgcattg atactctctg ccagatgttg tgtcgctttc acgcgcaaat
     8041 cacccagcaa caacgccacc gcttgcgggc ttcccagacg acaggcatcg ccaaatgccc
     8101 cccaacgcaa ctcttcgaga acgataaaac agcgcccagg caacgaccag gcgttatacg
     8161 ccccagcacg ccagcgggta aaaatttgct cgctctgatc acctgcctgc accagtaggc
     8221 ctcgttgcgt taacgccttt tccttccagc ggcaacgggc tgaaaaccac tcatacaagg
     8281 cggcaatttg ctgggggagc tccgggaaag cagagtgctg gcggtgatcc attttttgca
     8341 gcagcgccag atgctgcgcg atctgcgtca gtgccagatg accgggatcc agcatcgacg
     8401 ccagtttttc caccaatgtc agacgcaggc taagcggcgc atttaacatg ccgccgttcg
     8461 tccatgtctg aagctgcacg cgcgcaagaa gttcctgcat cagtcgttca cgaaactgct
     8521 gctgatgggc ttgtggaagg cgggcatcat cgccctgcgc cagatccact aaaaagcggg
     8581 gataaaccga ctccagcacg cgaccggggc cgtcaagtaa cgtcttggtc aatatcgttc
     8641 tgccgtgaaa agtgtttgaa tatcatcgcg taacagctgg gcgtcggtgt aaatccagcc
     8701 gtgagtcatc acagtccgct gcaattgctg ctgcatcagc ctgaccaccg attcattttg
     8761 ttgacgcaga gccagacttt cgcgtaaacg cgtctgtaat tccgtcaaac atgaagcgaa
     8821 ctcagcgaaa aaagtattca tgcctgccgt aacagattca tcgacctgct ctgccagaac
     8881 tttagccatt tgttggcaat aaagatcgac ttctgcgctt aaagctcgtt gcaacacact
     8941 gtaatcaacc gtctctgtcg gggatttctc atttccccgt ccccagtcgg gctgattcaa
     9001 ccagcgcgaa aaagtctcac gcacaacgcc taaacgcgtg ctctgctcgt ccgttgcttc
     9061 ctggcgcgaa atgacttcac tgaacagctg gcgagtgttg aagtggggaa ctgcgccgtg
     9121 aaaaacagga aaatgaaacc caggacgaaa ccctgactcg ctcaattcca ttttgacttg
     9181 ttgctcaatg gggcgaataa catcggttaa cacccggcaa agggtggatt ccagctcggc
     9241 aaaacgcagc gtaaagtcgc gactgatggt gttctgcgcc gtctgtaaca gtgtctcaca
     9301 gcgggtacga atctcgctta acggctccga atcatcctga aacaacgcgg ctaactgtgc
     9361 attcaccgca tcttgctgtt gacgcagaaa gtggttggcg gaggccaagg ccagctcgat
     9421 ttcatgttta atctcgccgc tcacctgcgc ctgattgagt tgcaatagct gcaaactttc
     9481 ttcgacctga tggatatttt gccgcaattg ttcacaagcg acgtttaacc cgtgcgcacg
     9541 aaaatccagg tattcccgcg cctgctgcgc gtaattcaac agtttatgcg cggcagatcg
     9601 cagagcatac aacgaggcgt tagcgtaagc ggcatgaagc aacgcctgaa ttggctgggc
     9661 gaacagcgaa tcttcccaca actgatcggc agcatgacga atatgttcga ggtccgccag
     9721 atcggcatga cgccagcgcc tgccgagcgc ggcatgggca aaatcttcca cccagcgttg
     9781 ttgctctggc ggtggtaact taccgttgtt ggctaactca tggcgcgccc gattcgccag
     9841 gtagccccac atcgacgaca ccggaaatat ctgctgtggc gtaatacagc ctttcatcag
     9901 cgtcccggaa atcagtgccc gcacctggtc ggcgtcgtca ctgttacgat cctgttgatc
     9961 gaacttattg accagcacat acagcggcac cgattgcccc accgccaaaa tcgcctcacg
    10021 gacctcttca tcggagatcg atttcagttg cgtataatcc agcaccgcca gtaccgccga
    10081 ggcgcgtgcc agctgctggt taagcatttt ttgcagatgc ggttgcccgg cttcatttgg
    10141 cccgggggta tccagtaacg tcaactgacc gggataactt tccagccccg ccagatggac
    10201 aaactccact tcaatcacgg gaatatgctc aatggcggcg taagcagaaa aaggaaaatc
    10261 gacgtccagc gccttcgcca gtcgcactaa atcattcaaa cttttcagac aatgaaaaat
    10321 aggctgggca cccagataat atttttcgaa agcgacgcca ttttcgatcc gctgcataag
    10381 cgcacgcata tctttatcta tttccagcac atcggtcaga tgcttaatat cgcaatcacg
    10441 caggcgctgt tgtaattttt gaattaaacg gtcgattggc gcgacatgtg aaaaatgcag
    10501 taccggttct ttttgcccgg gcgtatggcg aataagcgtc ggcagcgcag tcattgggcg
    10561 attacgatta ggcaaaacct ccgtaccaac aatggcatta atggtggttg atttccctgc
    10621 tttcatggta ccgacaatcg ccagcaccat ttccagtcgg gaaattttac gcaactcatt
    10681 attcagcatc gcgtgacgtt cggcgatatt aggctgactc cagggtaaag ccagttgtgg
    10741 cgcatcgtct ccgggtacag agagaggcat tttttccagt aactgcaact gttggcgaga
    10801 aagctgtaac aggcgttcag cctcctgact taactcatac agggtctgtg tgtacataga
    10861 aaattcttcc ttaaagcaaa ttttgttact tttatttagc cagattg
//
LOCUS       DAZGLJ010000152        10812 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000152 DAZGLJ010000000
VERSION     DAZGLJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.152"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(47..1711)
                     /gene="glnS"
                     /locus_tag="ACHK49_003470"
     CDS             complement(47..1711)
                     /gene="glnS"
                     /locus_tag="ACHK49_003470"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO9264059.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1914..3860)
                     /gene="nagE"
                     /locus_tag="ACHK49_003471"
     CDS             complement(1914..3860)
                     /gene="nagE"
                     /locus_tag="ACHK49_003471"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO9264060.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4193..4993
                     /gene="nagB"
                     /locus_tag="ACHK49_003472"
     CDS             4193..4993
                     /gene="nagB"
                     /locus_tag="ACHK49_003472"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO9264061.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5053..6201
                     /gene="nagA"
                     /locus_tag="ACHK49_003473"
     CDS             5053..6201
                     /gene="nagA"
                     /locus_tag="ACHK49_003473"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO9264062.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6210..7430
                     /gene="nagC"
                     /locus_tag="ACHK49_003474"
                     /gene_synonym="nagR"
     CDS             6210..7430
                     /gene="nagC"
                     /locus_tag="ACHK49_003474"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO9264063.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7478..8230
                     /gene="nagD"
                     /locus_tag="ACHK49_003475"
     CDS             7478..8230
                     /gene="nagD"
                     /locus_tag="ACHK49_003475"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO9264064.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8487..10151
                     /gene="asnB"
                     /locus_tag="ACHK49_003476"
     CDS             8487..10151
                     /gene="asnB"
                     /locus_tag="ACHK49_003476"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO9264065.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10532..10608
                     /locus_tag="ACHK49_003477"
     tRNA            10532..10608
                     /locus_tag="ACHK49_003477"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10566..10568,aa:Met,seq:cat)
     gene            10617..10701
                     /locus_tag="ACHK49_003478"
     tRNA            10617..10701
                     /locus_tag="ACHK49_003478"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10651..10653,aa:Leu,seq:tag)
     gene            10725..10799
                     /locus_tag="ACHK49_003479"
     tRNA            10725..10799
                     /locus_tag="ACHK49_003479"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10757..10759,aa:Gln,seq:ttg)
BASE COUNT         2677 a         2808 c         2758 g         2569 t
ORIGIN      
        1 gccggatgcg acgtaaatgc ctcacccggc ctggtagaac ttaaaattac tcgcctactt
       61 tcgcccaggt atcacgcagc ccaacggtgc ggttaaatac cggtttttcc gctgtggaat
      121 ggcggctgtc gaggcagaag taaccttcac gctcaaactg gaatgcttta cccgcaaccg
      181 catctttcag cgacggttca gcaaagccct gtttgatcac cagcgattcc gggttaatca
      241 ccgacaggaa atcatccgca gcaccagggt tcggcacgct gaacagacgg tcgtacagac
      301 ggatttcaac cggcagcgca tgtgccgcgc tcacccagtg aataacacct ttgactttac
      361 gaccatctgc cggatctttg cttaaggtgt cggcgtcata agtacagaag atggtggtga
      421 tattgccttc ggcatctttc tccacgcgtt cagccttaat gacgtaagca ttacgcagac
      481 gcacttcttt acccagcacc agacgtttgt actgcttgtt agcttcttcg cggaaatcgg
      541 cgcgatcgat ccaaatctca ccgctaaacg gcacctgacg actccccatt tccggtttgt
      601 tcggatggtt cggcatggtg accatttcgc cttcgccctg atagttttcg ataaccagtt
      661 tcaccggatc gataaccgcc attgcgcgcg gcgcattttc gttgagatct tcacggatgc
      721 aggattccag cgatgccatc tcaatggtgt tgtcctgctt ggtcacgccg atgcgtttgc
      781 agaactcacg aatagaagca gcagtgtaac cacgacgacg cagaccggaa atggtcggca
      841 tacgcgggtc atcccagcct tcaacgtgct tgtcggtcac cagcaggttc agcttacgct
      901 tggacatcac agtgtattcc agattcaggc gcgagaattc gtactggcgc gggtgaacag
      961 gaatggtgat gttgtccagc acccagtcgt acaggcgacg gttgtcctgg aactcaagcg
     1021 tacacagaga gtgcgtaata ccttccaggg catcgctgat gcagtgggtg aagtcgtaca
     1081 tcgggtagat gcaccacttg ttgccagtct ggtggtgttc agcaaattta atacggtaca
     1141 gcaccggatc gcgcatcacg ataaacggtg aagccatgtc gattttcgca cgcaggcagg
     1201 ctttaccttc ttcaaaacca ccggcacgca ttttttcgaa cagcgccagg ttctcttcaa
     1261 cgctgcggtc gcggtacggg ctgtttttac ccggttgcgt cagggtgccg cggtattcgc
     1321 ggatctgttc cggcgtcagt tcatcaacgt acgccaggcc tttattgatc agttcgatcg
     1381 cataggcgtg gagctgatca aaataatcgg aggagtaacg gacgttacca gaccagtgaa
     1441 aacctaacca ctctacgtcg tttttgatcg actcaacata ctcgatatct tcttttaccg
     1501 ggttagtgtc gtcgaaacgc aggttgcact ggcctttata gtcctgggcg atcccgaagt
     1561 tcaggcagat agatttcgca tggccaatat gcagatagcc attcggctcc ggcgggaaac
     1621 gggtgtgtac tgtggtgtgc ttaccactgg ccagatcttc atcgatgatc tgacggataa
     1681 agttagtcgg gcgggcttct gcctcactca tcgtggattc ctcaaagcgt aaacaacgta
     1741 taacggcgta tgatcttata agcgggacag gctgacaact gttagttttt taaaattgat
     1801 aatctaacaa ggattatggg gatgattgat gaaaaaaaaa gcggcagaga agatctccac
     1861 cgcttgtcat tgttggatgc gacgctcaag cgtcgcatca ggcataaagc agattacttt
     1921 ttgatttcat acagcggtgt ttgacccgcc acaacatggc cctgagcttt aatgatcaag
     1981 ccactgaaat cgtcgatatt gctgcaaacc accgggctaa tcatcgagcg ggcgttagcg
     2041 ttcaggtaat ccagatccat ttccagaatc ggttgccctg cgcttacctg cgcaccctct
     2101 tccaccagac gtttaaagcc tttaccttcc agcgctacgg tgtcgatacc catatggacg
     2161 acgatctccg cgcctttttc ggtttccagg cagaacgcgt ggttggtgtt gaagattttc
     2221 acgattgtcc ctgcggctgg tgatacgacg attttatctg tcggtttcac cgccacaccg
     2281 tcacccaccg ctttgctggc gaatgcttcg tcaggaacct gatccagtgc cacgacatca
     2341 ccggtaatcg gcgataccag ctccgcgata gataccgcgt ttggtacagc ctgcggtttt
     2401 gctacaggcg cggcagttgc cggagttgct tcagctgacg cagcggctac cggaccacgg
     2461 gcaacgactt tcttcatcgc atcgccgatg gattctgctt tcgcgccaac aatcacctga
     2521 atagtctgtt tgttcagttt cactacacca gaagcaccca gacgtttaca catcgtatcg
     2581 ttgacgcggg cagagtcagc cacggtcagg cgcagacggg tgatacaggc gtcaattgct
     2641 ttcaggttgt cagtgccgcc aaccgcagca atatagttgg tcgccagttg attgaggcct
     2701 tcttcagtgt tgctgttggc ttcttcagta acgatctcgt cttctttatc ttcacgaccc
     2761 ggcgttttca ggttgaacat gcggataacc aaactgaaca ccacgaagta gatagcgaag
     2821 aagacaacac ccataaccag cagcatccag acgttctggc tggcggccgg caggttatac
     2881 atcaacgcgt agtcgatagc ccccgctgag aaagagaagc cagcatggat acccagcagt
     2941 gttgccacaa acaggctgat accggtcagc agtgcgtgca ggaggtacag cagcggtgca
     3001 aggaacatga acaggaattc cagcggctca gtcacaccgg tcaggaacgc agtaacagca
     3061 acagaaagca gcatcccgcc aaccatcgga cgacgctctt tcggtgctgc gaagtacatc
     3121 gccagcgccg cacccggcag accgaacatc ataatcggga agaagccgga catgaacatc
     3181 cccgcggtgc cgtcaccggc atagaagcgg ttgatatcac cgtggaaaac cgtacccgcc
     3241 gcgttggtga attcaccaat ctggaaccag gcgatggtgt tcagcacctg atgcagaccg
     3301 gttgggatca gcagacggtt gatgaaacca aagataccgg aacccagcgc gcccgcagaa
     3361 acgatccact cgcctcctgc atggatagcg tgctgtaccg gcggccagac gtaaccaaaa
     3421 atggccgcca gcaccaggca gaagaagccg gtggcaatcg gcacaaagcg tttgccgccg
     3481 aagaaactca ggaagtccgg cagtttaata tcggaccaac ggttataggc tgcgccacca
     3541 accagaccgg taatgatacc cgccagtaca cccatgttaa tttctgggtt gatggtcacc
     3601 atcgctttgg ttaacacaaa gtaacctacc gcacccgcca gcgccgccgc acctgcgctg
     3661 tctttcgacc agctggatgc cacaccgatg gcgaagatta atgcgaggtt atcaaaaatc
     3721 gcaccgcccg cctgggcaat aaacgcaacg ttaagtaaat ctggctgacc gaatcgcagc
     3781 aacagtgccg ccaccggcag caccgcgata gggagctgta acgccctacc gagtcgctgg
     3841 aaaaaaccta aaatattcat cttattcccc ctacgagaac cctatttggc tcgtttaaag
     3901 ccgtattttt attttgctgc aaattgtact gccgatgttc tgtaatcaga ttgttagatc
     3961 atctgctaca gagtgtgtga aaatttaatt cgtatcgcaa attaaacgcg tgtcttttgt
     4021 gagttttgtc accaaatatc gttatcatca ctccctttta ctggctaaag cagaaaactt
     4081 attttatcat tcaaaaaatc aggtcggatt gacgcctgtc tgcgcaaatc caggttacgc
     4141 ttaaagatgc ctaatccgcc aacggcttac attttactta ttgaggtgaa taatgagact
     4201 gatccccctg actaccgctg aacaggtcgg caaatgggct gctcgccata tcgtcaatcg
     4261 tatcaatgcg ttcaaaccga ctgccgatcg tccgtttgta ctgggcctgc cgactggcgg
     4321 cacgccgatg accacctata aagcgttagt cgaaatgcat aaagcaggcc aggtcagctt
     4381 taagcacgtt gtcaccttca acatggacga atatgtcggt ctgccgaaag agcatccgga
     4441 aagctactac agctttatgc accgtaattt cttcgatcac gttgatattc cagcagaaaa
     4501 catcaacctt ctcaacggca atgccccgga tatcgacgcc gagtgccgcc agtatgaaga
     4561 aaaaatccgt tcttacggaa aaattcatct gtttatgggc ggtgtaggta acgacggtca
     4621 tattgcattt aacgaaccgg cgtcttctct ggcttctcgt actcgtatca aaaccctgac
     4681 tcatgacact cgcgtcgcga actctcgttt ctttgataac gatgtcaatc aggtgccaaa
     4741 atatgccctg actgtcggtg ttggtacatt gctggatgcc gaagaagtga tgattctggt
     4801 gctgggtagc cagaaagcac tggcactgca ggctgcagtt gaaggttgcg tgaaccacat
     4861 gtggaccatc agctgtctgc aactgcatcc aaaagcgatc atggtgtgcg atgaaccttc
     4921 caccatggag ctgaaagtta agactttaag atatttcaat gaattagaag cagaaaatat
     4981 caaaggtctg taattgttat ccctgccctg gctccttgct cagggcaata ttttttaaaa
     5041 tcgggggtca gaatgtatgc attaacccag ggccggatct ttaccggcca cgaatttctt
     5101 gatgaccacg cggttgttat cgctgatggc ctgattaaaa gcgtctgtcc ggtagcggaa
     5161 ctgccgccag agatcgaaca acgttcactg aacggggcca ttctctcccc cggttttatc
     5221 gatgtgcagt taaacggctg cggcggcgta cagtttaacg acaccgctga agcggtcagc
     5281 gtggaaacgc tggaaatcat gcagaaagcc aatgagaaat caggctgtac taactatctg
     5341 ccgacgctta tcaccaccag cgatgagctg atgaaacagg gcgtgcgcgt tatgcgcgag
     5401 tacctggcaa aacatccgaa tcaggcgtta ggtctgcatc tggaaggtcc gtggctgaat
     5461 ctggtaaaaa aaggcaccca taatccgaat tttgtgcgta agcctgatgc cgcgctggtc
     5521 gatttcctgt gtgaaaacgc cgacgtcatt accaaagtga ccctggcacc ggaaatggtt
     5581 cctgcggaag tcatcagcaa actggcaaat gccgggattg tggtttctgc cggtcactcc
     5641 aacgcgacgt tgaaagaagc aaaagccggt ttccgcgcgg ggattacctt tgccacccat
     5701 ctgtacaacg cgatgccgta tattaccggt cgtgaaccgg gcctggcggg cgcgatcctc
     5761 gacgaagctg acatttattg cggtattatt gctgatggcc tgcatgttga ttacgccaac
     5821 attcgtaacg ctaaacgtct gaaaggcgac aaactgtgtc tggttaccga cgccaccgcg
     5881 ccagcaggtg ccaacattga acagttcatt tttgcgggta aaacaatata ctaccgtaac
     5941 ggactttgtg tggatgagaa cggtacgtta agcggttcat ccttaaccat gattgaaggc
     6001 gtgcgtaatc tggtcgaaca ttgcggtatc gcactggatg aagtgctgcg tatggcgacg
     6061 ctctatccgg cgcgtgcgat tggcgttgag aaacgtctcg gcacactcgc cgcaggtaaa
     6121 gtagccaacc tgaccgcatt cacacctgat tttaaaatca ccaagaccat cgttaacggt
     6181 aacgaggtcg taactcaata agagaaagta tgacaccagg cggacaagct cagataggta
     6241 atgttgatct cgtaaaacag cttaacagcg cggcggttta tcgcctgatt gaccagtacg
     6301 ggccaatctc gcggattcag attgccgagc aaagccagct tgcccccgcc agcgtaacca
     6361 aaattacgcg tcagcttatc gaacgcgggc tgatcaaaga agttgatcag caggcctcca
     6421 ccgggggccg ccgcgctatc tccatcgtca ccgaaacccg caatttccac gcaatcggcg
     6481 tacggcttgg tcgtcacgac gccaccatca ccctgtttga tctcagcagc aaagtgctgg
     6541 cagaagaaca ttacccgctg ccggaacgta cccagcaaac gctggaacat gccctgttga
     6601 atgccattgc tcagtttatt gatagctacc agcgcaaact gcgcgagctg atcgcgattt
     6661 cggtgatcct gccagggctt gttgacccgg acagcggcaa aattcattac atgccgcata
     6721 ttcaggtaga aaactggggg ctggtagaag cactggaaga acgttttaaa gtgacctgtt
     6781 tcgttggtca cgatatccgt agtctggcgc tggcggagca ctacttcggt gcaagtcagg
     6841 attgcgaaga ctccattctg gtgcgtgtcc atcgcggaac cggggccggg attatctcta
     6901 acgggcgcat ttttattggc cgcaacggca acgtcggtga aattggccat attcaggtcg
     6961 aaccgctggg tgaacgctgc cactgcggca actttggctg cctggaaact atcgctgcca
     7021 acgccgctat tgaacaacgg gtgttgaatc tgttaaagca gggctaccag agccgcgtgc
     7081 cgctggacga ctgcaccatc aaaactatct gcaaagccgc gaacaaaggc gatagcctgg
     7141 cctcggaagt gattgagtat gtcggtcgtc atctgggcaa aaccatcgcc attgctatca
     7201 acttatttaa tccgcaaaaa attgttattg ccggtgaaat caccgaagcc gataaagtgc
     7261 tgctccctgc tattgaaagc tgcattaata cccaggcgct gaaggcgttt cgcactaatc
     7321 tgccggtggt acgttctgag ctggatcacc gctcggcaat cggcgctttt gcgctggtaa
     7381 aacgcgccat gctcaacggt attttgctcc agcatttgct ggaaaattaa tgtgctttta
     7441 tagtggcgct tattgttgtc aatattctgg gtagtccatg accattaaaa atgtaatttg
     7501 cgatatcgac ggcgtgctga tgcacgataa cgtcgccgta ccgggtgcag cggaattttt
     7561 gcacgggatt atggataaag gcctgccgct ggtgttgctg accaactatc cttcgcagac
     7621 cgggcaagat ctggcgaacc gctttgccac tgccggtgtc gatgtgccgg atagcgtgtt
     7681 ttatacctct gcgatggcga ctgccgattt cctgcgtcgc caggaaggca agaaagcgta
     7741 tgtggtgggc gaaggcgcac tgattcatga actgtacaaa gccggtttca ctattaccga
     7801 tgtgaaccct gattttgtga ttgttggcga aacgcgttcc tacaactggg acatgatgca
     7861 taaagcggcc tatttcgtcg ctaacggtgc acgctttatc gccaccaacc cggacaccca
     7921 cgggcgcggt ttttatcccg cttgtggcgc gttgtgtgca gggattgaga aaatctccgg
     7981 gcgcaaaccg ttctatgttg gtaagcccag cccgtggatc atccgcgcgg cattaaacaa
     8041 aatgcaggcg cattcggaag aaacggtgat tgtcggcgat aacttgcgca ccgacattct
     8101 ggcgggcttc caggcgggtc tggagacgat tctggtgctt tctggtgttt catcgctcga
     8161 cgatatcgac agtatgccgt tccgcccaag ctggatttac ccgtcggtcg ctgaaatcga
     8221 cgttatctga atagcaacca cgtctcaggg cgtggtttct tatttttacc catcattaaa
     8281 atcatcccat tcaataaaac tcgccaaaaa ttatcgatcc atcaataaat acatcaccac
     8341 accctcccct ttttcacaaa tcagcaatca ccattacgtt ttttattttt gcactgccaa
     8401 atcgcttgcg cacggtgctg ctttgcggca tttttttaaa caagcaaaca caacaagcaa
     8461 caaataccag gttaacggag aaggttatgt gttcaatttt tggcgtattc gatatcaaaa
     8521 cagacgcagt tgagctgcgt aagaaagcac tcgagctgtc acgcctgatg cgtcatcgtg
     8581 gcccggactg gtccggtatt tatgccagcg ataacgccat tctcgcccac gaacgtctgt
     8641 caattgttga cgttaacgcg ggggcgcaac ctctctacaa ccaacaaaaa actcatgtgc
     8701 tggcggtaaa cggtgaaatc tacaaccacc aggcactgcg cgccgaatat ggcgatcgtt
     8761 atcagttcca gaccggatct gactgcgaag tgatcctcgc gctgtatcag gaaaaagggc
     8821 cggaatttct cgacgacttg cagggcatgt ttgcctttgc cctgtacgac agcgaaaaag
     8881 atgcatatct gattggtcgc gaccatctgg ggatcatccc actgtatatg ggctatgacg
     8941 agcacggtca gctgtatgtg gcctcagaaa tgaaagccct ggtgccagtt tgccgcacga
     9001 ttaaagagtt cccggcgggg agctatttgt ggagtcagga cggcgaaatc cgttcttatt
     9061 atcatcgcga ctggttcgac tttgatgcag tgaaagataa cgtgaccgac aaaaacgagc
     9121 tgcgtcaggc actggaagat tccgttaaaa gccatctgat gtctgacgtt ccttacggtg
     9181 tgctgctctc gggtggtctg gattcctcaa ttatttccgc gatcaccaag aaatacgccg
     9241 cccgtcgcgt ggaagatcag gaacgctctg aagcctggtg gccgcaatta cactcctttg
     9301 ctgtaggtct gccgggttca ccggatctga aagcagccca ggaagtggca aaccatctgg
     9361 gcacggtgca tcacgaaatt cacttcactg tacaggaagg tctggatgcc atccgcgacg
     9421 tgatttacca catcgaaacc tatgatgtga caactattcg cgcttcaaca ccgatgtatt
     9481 taatgtcgcg taagatcaag gcaatgggca ttaaaatggt gctgtccggt gaaggttctg
     9541 atgaagtgtt tggcggttat ctttacttcc acaaagcacc gaatgccaaa gaactgcatg
     9601 aagagacggt gcgtaaactg ctggccctgc atatgtatga ctgcgcgcgc gccaacaaag
     9661 cgatgtcagc ctggggcgtg gaagcacgcg ttccgttcct cgacaaaaaa ttccttgatg
     9721 tggcgatgcg cattaacccg caggataaaa tgtgcggtaa cggcaaaatg gaaaaacaca
     9781 tcctgcgtga atgttttgag tcatacctgc ccgcaagcgt ggcctggcgg cagaaagagc
     9841 agttctccga tggcgtcggt tacagttgga tcgacaccct gaaagaagtg gcggcgcagc
     9901 aggtttctga tcagcaactg gaaactgccc gcttccgctt cccgtacaac acgccaacct
     9961 caaaagaagc gtatctgtac cgggagatct ttgaagaact gttcccgctt ccgagcgccg
    10021 ctgagtgcgt gcctggcggt ccttccgtcg cgtgttcttc cgctaaagcg attgagtggg
    10081 atgaggcgtt caagaaaatg gacgatccgt ctggtcgtgc ggttggcgtt caccagtcgg
    10141 cgtataagta agaaatgcga aacagccccg gtgaaatccg gggctgtttc agttattgag
    10201 gtgcttgtta attctgatat gcagtaagga aatgttccgg gtttgccaca tcactgtttg
    10261 acaactcatc actgtaggcc agtatttcat agattaattt cccaccacta ttgttgaatt
    10321 ccgaatttca tcctgtacga caaaagaatg gctgtagggt ggggaaaaaa cacaaagtaa
    10381 acaataattg acgaatatag cgccacgctg ttcgcaacct aaccaaacag tcactttcga
    10441 gcaattttcc ttgaaaaaga ggttgacgct gcaaggctct atacgcataa tgcgccccgc
    10501 aacgccgata aggtatcgcg aaaaaaaaga tggctacgta gctcagttgg ttagagcaca
    10561 tcactcataa tgatggggtc acaggttcga atcccgtcgt agccaccatc ttttttgcgg
    10621 gagtggcgaa attggtagac gcaccagatt taggttctgg cgccgcaagg tgtgcgagtt
    10681 caagtctcgc ctcccgcacc attcaccaga aagcgttgta cggatggggt atcgccaagc
    10741 ggtaaggcac cggtttttga taccggcatt ccctggttcg aatccaggta ccccagccat
    10801 cgaagaaaca at
//
LOCUS       DAZGLJ010000153        10757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000153 DAZGLJ010000000
VERSION     DAZGLJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.153"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1410
                     /gene="cscA"
                     /locus_tag="ACHK49_003480"
     CDS             <1..1410
                     /gene="cscA"
                     /locus_tag="ACHK49_003480"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO9264066.1"
                     /translation="AQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLIWFNDRYHA
                     FYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSAVDDNGVLS
                     LIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFRDPKVWREA
                     DTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPDFFSLGDQH
                     YLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDFYAPQSFLA
                     KDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEAESLRQQHQ
                     SVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDNQSERLVLW
                     RYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYPQPEERELS
                     LYASHGVAVLQHGALWLLG"
     gene            complement(1418..2413)
                     /locus_tag="ACHK49_003481"
     CDS             complement(1418..2413)
                     /locus_tag="ACHK49_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9264067.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2656..2874
                     /locus_tag="ACHK49_003482"
                     /pseudo
     CDS             2656..2874
                     /locus_tag="ACHK49_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            2892..4220
                     /gene="dsdA"
                     /locus_tag="ACHK49_003483"
     CDS             2892..4220
                     /gene="dsdA"
                     /locus_tag="ACHK49_003483"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO9264068.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKMTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4328..5866)
                     /gene="emrY"
                     /locus_tag="ACHK49_003484"
     CDS             complement(4328..5866)
                     /gene="emrY"
                     /locus_tag="ACHK49_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO9264069.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(5866..7029)
                     /gene="emrK"
                     /locus_tag="ACHK49_003485"
     CDS             complement(5866..7029)
                     /gene="emrK"
                     /locus_tag="ACHK49_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO9264070.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7445..8059
                     /gene="evgA"
                     /locus_tag="ACHK49_003486"
     CDS             7445..8059
                     /gene="evgA"
                     /locus_tag="ACHK49_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO9264071.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8064..>10757
                     /gene="evgS"
                     /locus_tag="ACHK49_003487"
     CDS             8064..>10757
                     /gene="evgS"
                     /locus_tag="ACHK49_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO9264072.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNSDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQT"
BASE COUNT         3099 a         2432 c         2366 g         2860 t
ORIGIN      
        1 gcgcaaaacg cactagcaaa acttcatgag caccggggta acactttcta tccccatttt
       61 cacctcgcgc cacctgccgg atggatgaac gatccaaacg gcctgatctg gtttaacgat
      121 cgttatcacg cgttttatca acatcacccg atgagcgaac actggggacc aatgcactgg
      181 ggacatgcca ccagcgacga tatgatccac tggcagcatg agcctattgc gctagcgcca
      241 ggagacgata atgacaaaga cgggtgtttt tcaggtagtg ctgtcgatga caatggtgtc
      301 ctctcactta tctacaccgg acacgtctgg ctcgatggtg caggtaatga cgatgcaatt
      361 cgcgaagtac aatgtctggc taccagtcgg gatggtattc atttcgagaa acagggtgtg
      421 atcctcactc caccagaagg aatcatgcac ttccgcgatc ctaaagtgtg gcgtgaagcc
      481 gacacatggt ggatggtagt cggggcgaaa gatccaggca acacggggca gatcctgctt
      541 tatcgcggca gttcgttgcg tgaatggacc ttcgatcgcg tactggccca cgctgatgcg
      601 ggtgaaagct atatgtggga atgtccggac tttttcagcc ttggcgatca gcattatctg
      661 atgttttccc cgcagggaat gaatgccgag ggatacagtt accgaaatcg ctttcaaagt
      721 ggcgtaatac ccggaatgtg gtcgccagga cgactttttg cacaatccgg gcattttact
      781 gaacttgata acgggcatga cttttatgca ccacaaagct ttttagcgaa ggatggtcgg
      841 cgtattgtta tcggctggat ggatatgtgg gaatcgccaa tgccctcaaa acgtgaagga
      901 tgggcaggct gcatgacgct ggcgcgcgag ctatcagaga gcaatggcaa acttctacaa
      961 cgcccggtac acgaagctga gtcgttacgc cagcagcatc aatctgtctc tccccgcaca
     1021 atcagcaata aatatgtttt gcaggaaaac gcgcaagcag ttgagattca gttgcagtgg
     1081 gcgctgaaga acagtgatgc cgaacattac ggattacagc tcggcactgg aatgcggctg
     1141 tatattgata accaatctga gcgacttgtt ttgtggcggt attacccaca cgagaattta
     1201 gacggctacc gtagtattcc cctcccgcag cgtgacacgc tcgccctaag gatatttatc
     1261 gatacatcat ccgtggaagt atttattaac gacggggaag cggtgatgag tagtcgaatc
     1321 tatccgcagc cagaagaacg ggaactgtcg ctttatgcct cccacggagt ggctgtgctg
     1381 caacatggag cactctggct actgggttaa cataatatca ggtggaacaa cggatcaaca
     1441 gcgggcaagg gatccgcgtc actcttcccc cttcacgacc ttcaataata tgcaatgcag
     1501 cttcccgccc gataatgtca tgtggaagct gaattgtggt cagcggcggt aaaaacagat
     1561 gcccgacgcc aaccagatta tcaaagccca ttacggcgac atcctgcggg attcgtaccc
     1621 ccttcgccag aagaacctga taagccacaa aggctgcgcg atcgttacca catatcagaa
     1681 catcaaaatc tggtttgccc gatttgaagt ggtcattgag taaacttgcg agatcggtgt
     1741 agtgatcatc acctgttgcc atgtgaaatt gtttcacctc agccagatct cgtccagcat
     1801 cacgccaggc ctgctcaaat ccctgccgac gataccctgt tgccaacgca ctttccggta
     1861 gccagaagca taacggttga cgatagcccg ccgcgagcaa atgctgtgtt gattcatatt
     1921 gtgcagtgta atcatcaggg atataactgg gtaacgctgg gtcatccgcc acacagttcg
     1981 ccaatacaat attttcacca tacagagact caggcagcgt gatatgtcgc agccccattg
     2041 tagtatagat aatgccatcc ggacggtggg caagcagctg acgtgccgcg cgggcagcgt
     2101 catcttcaga aaaaatattg attaaaaaac tattccagcc gaactcgctg gcggtttgct
     2161 caatggcaag cagaatatca acagagaaag gagtggtagc cgtgtcctgc gccagcacgg
     2221 cgagagtcga cggcttacgt ccttgagcgc gcatcttacg ggcggaaaga tcaggaacat
     2281 aattcagggt ctggattgcc tgcaatacgc ggtcacgcgt tgcaggacgc acagattctg
     2341 cattatgcat cacccgggag actgtcatca tcgacactcc cgctaggcgt gcgacatcct
     2401 ttaatgaagc catacccaag ccgtttgccg taaaacgggc actgtagcag aaacagacgt
     2461 cactggcgag atccaacgat attctcaacg ccctatcacc cgacacggca atacaataaa
     2521 aaataacaat aattgccgga taattgtact caattccgcc tctgttctcg ccaacgagta
     2581 accatatagg caagtaagta aaacatgcca actggcatcg ccaacctgga gaagaaaata
     2641 taaatatgga tcattgtgct gccgctgtat cccgacatca gcccggaaat tattgcgatt
     2701 gctatcggtt caggtgcaat tggctgcacg atcgttacgg actcgctttt ctggctagtg
     2761 aagcaatatt gcggcgctac gctcaatgaa acatttaaat actatacgac agcgacattt
     2821 atcgcttcag tcgtcgctct ggcgggcaca ttcctgctgt catttatcat ctaagcgcaa
     2881 agagacgtac tatggaaaac gctaaaatga actcgctcat cgcccagtat ccgttggtaa
     2941 aggatctggt tgctcttaaa gaaaccacct ggtttaatcc tggcacgacc tcattggctg
     3001 aaggtttacc ttatgttggc ctgaccgaac aggatgttca ggacgcccat gcgcgcttat
     3061 cccgttttgc accctatctg gcaaaagcat ttcctgaaac tgctgccact ggggggatta
     3121 ttgaatcaga actggttgcc attccagcta tgcaaaaacg gctggaaaaa gaatatcagc
     3181 aaccgatcag cgggcaactg ttactgaaaa aagatagcca tttgcccatt tccggctcca
     3241 taaaagcacg cggcgggatt tatgaagtcc tggcacacgc agaaaaactg gctctggaag
     3301 cggggttgct gacgcttgat gatgactaca gcaaactgct ttctccggag tttaaacagt
     3361 tctttagcca atacagcatt gctgtgggct caaccggaaa tctggggtta tcaatcggca
     3421 ttatgagcgc ccgcattggc tttaagatga cagttcatat gtctgctgat gcccgggcat
     3481 ggaaaaaagc gaaactgcgc agccatggcg ttacggtcgt ggaatatgag caagattatg
     3541 gtgttgccgt cgaggaagga cgtaaagcag cgcagtctga cccgaactgt ttctttattg
     3601 atgacgaaaa ttcccgcacg ttgttccttg ggtattccgt cgctggccag cgtcttaaag
     3661 cgcaatttgc ccagcaaggt cgtatcgtcg atgctgataa ccctctgttt gtctatctgc
     3721 cgtgtggtgt tggcggtggt cctggtggcg tcgcattcgg acttaaactg gcgtttggcg
     3781 atcatgttca ctgctttttt gccgaaccaa cgcactcccc ttgtatgttg ttaggcgtcc
     3841 atacaggatt acacgatcag atttctgttc aggatattgg tatcgacaac cttaccgcag
     3901 cggatggcct tgcagttggt cgcgcatcag gctttgtcgg gcgggcaatg gagcgtctgc
     3961 tggatggctt ctataccctt agcgatcaaa ccatgtatga catgcttggc tggctggcgc
     4021 aggaagaagg tattcgtctt gaaccttcag cactggcggg tatggccgga cctcagcgcg
     4081 tgtgtgcatc agtaagttac caacagatgc acggtttcag cgcagaacaa ttgcgtaatg
     4141 ccactcatct ggtgtgggcg acgggaggtg gaatggtgcc ggaagaagag atgaatcaat
     4201 atctggcaaa aggccgttaa taacgtttca acgcagcatc gcaatccttt ccctgggtga
     4261 gcgatgctgc cgatggcgca gacttaagat ccccgctctt acccgctata accccctcct
     4321 tttatcatca cccaacgcct ttcgctgtaa acggcggttt cgcaaaccaa accaaaaccg
     4381 taagtaagat aaaagcaata gccgccatac ggaaaatctc atttgcagaa atagaaagtg
     4441 actgctgggt tatttcatta ttaatttcat taagaactcc tgaaagcgaa ccatagtatt
     4501 tatccataat ttgtgacgat gaattaaaca cggggttaaa ttgatcgatg gttgctgtca
     4561 actgactatg gtgtaacgat tcgcgtcgtc cccacagcgt cattgtcaac gacgtaccaa
     4621 ctgatcctga caaggtacga aaaaaattac tcatactcga ggcattggca aatttattat
     4681 ctggcaagcc tgaaaacgaa atcgttgtta agggtaaaaa gaaacaggca acggcgaatc
     4741 cctggaaaaa ctgtggcaaa atgatgcctg taaaatcaat cgttggcata aatgtcacag
     4801 aacgccagta atagcaaacc gcatacatca aaaaactaaa tgtcactaac aaccgcatgt
     4861 ctattttgtt gccataacgt cctatcaaag gtgaaattaa tagtggcatg atgccgatgg
     4921 gcgcataagc aagtccggcc catatcgcat tataccccat cgtttcctgg agtaactgcg
     4981 gcataaggac gatcgctcca gagtaaaata aatacgcgca tgtgatactc acaataccaa
     5041 tggtgaagtt acgggactta aacaaactga gatcaagaat cgggttctct gaggtcgact
     5101 cccaaatgac taaagagatc agagaaataa ctgatactac tgttaatatt attattgtac
     5161 tcgagttgaa ccaatccaga tcgcgccctt tatcaagcat aatttgcaag ccaccaacac
     5221 cgagcactaa cagggtcagt cctggtagat tcattttgac cggtgaagtt tcagtttctc
     5281 ttcctttaag taaggttaag cataatgtca ggacgataat ccccataggg acattgatta
     5341 aaaatatcca accccagcta aagttatcgc aaatataacc gcccaatatc ggcccacata
     5401 tcggagcgat aatcacggtc attgaccata atgccagagc aaatgttctt ttttctggtg
     5461 gataattcct taataataaa ctctgtgaca gtggaattaa cggccccgcc attaatccct
     5521 gaacgactct aaaaaatatc agcacatcaa gattggtcga taaactacac attaaggaag
     5581 acagcgaaaa aaaagtgact gaaagtaaaa ataaccttaa ttcgcctatt ctttgtgcca
     5641 gcctgccggt aacagggatc gcaatggcat ttgctacgcc aaacgaggtg ataacccagg
     5701 tgccttcgtc tgttgatgct cccagaaagc cagatattgt cggtattgcg acgttagaaa
     5761 tagtggaatc caacatttgc ataaacgtcg ctaatgacaa tgcaatagta acgcaccata
     5821 acgtcccacc ggttaatggt gccggagtcg atttagtgat tgccattaaa gttgtccatt
     5881 atgcgaaata atgttgctaa tttctttttc tatcggactg gtatcgataa ctaaagcctt
     5941 actggtataa gccggcatgg acgtcacggt tgaagccaga tcaggcatct cagcaatgtc
     6001 ttcgttcttc gtatcaatag ttgcagtcat cgataaacca atgcgtaagg ggtgttccat
     6061 gagttctttt ggatcaagag aaacttcaac cggtacacgc tgaacgattt tgatccagtt
     6121 ccctgtcgca ttttgtgcag gtaataagga gaacgcattg ccggttccca tattgatccc
     6181 tgtcacccga ccatgaaaca caacattttc accataaaga tcgctgataa tattgactga
     6241 ttgaccaatc cgtacatccg tgagttgtgt ttctttaaag ttggcattaa cccacatttg
     6301 acgtgccggt acgacagcca ttaacgattg tccgggactc actgtttcgc cgacctgaac
     6361 actccgctgg gcaatatagc cagtaaccgg actccttata tccgtacgtt taagcgccaa
     6421 ccaggcttct ttagttgcat ccgccgcttc cacgacttgt ggctgacggt ttaatggtgt
     6481 gttcattact aaagctttat tcgctttata agcctggata gcggcattca gtgccgcttt
     6541 gctacttatt aacgtatctt tggtatgctc cagcgtttct tttgaaataa ccccctgctt
     6601 cgctaacggt actcgacggt tataatcttc taaagattgt tgatactgaa tacgtgctga
     6661 agcgacttcg gcactgtatt gtttatcctg taagtacagt ttattcgttt gccgaacaat
     6721 atttgccaga ttatttttag ctttattgag tgcgatagtg gcatcagttt tatccagtga
     6781 aactaaaatg tcaccttgtc gaacgtagtt cgtatcttta tgattaacga cagtgacact
     6841 acctgagact tgtgcagaaa ttggatctgc attccccgtg acataggcgt catctgtact
     6901 aatcatgtct tctaattcca ttgaccaata ggcataggca cctgaaaacg caataaataa
     6961 aactactgcc aataaagaaa agtattttct tctgttagaa tgttttttat ttgaattaat
     7021 ctgttccact attatctctc atttctcata gatgaaattt atgaattgaa tgaaaagagc
     7081 attcattctt catatcaaac acagtgcatg tgttgcacat tatcccagga acgttggagg
     7141 cctaatttaa atgaggtgca aaaaaaacaa cagaggacta aaccgtggct tttgcaatac
     7201 aattcttacg cctgtaggat tagtgagaag acttatagtg ctaacttgaa actataaatc
     7261 atcggtataa tccctgattt tattgttgac atttcattta tgccgactat ttatatggta
     7321 tacttgtcga attatcttaa aggaagctca gattttctta tttttattga gaaaatgaga
     7381 tgacgcctta tgtctgtatt actacaggga gaagggagat gcttcattgc aaagggaata
     7441 atctatgaac gcaataatta ttgatgacca tcctcttgct atcgcagcaa ttcgtaattt
     7501 attgatcaaa aacgatattg aaatcttagc ggagttgact gaagggggaa gcgccgttca
     7561 gcgggtggaa acacttaagc ctgatatcgt catcattgat gtcgatatcc ccggagttaa
     7621 cggtatccag gtgttagaaa cgctgaggaa gcgccaatat agcggaatta ttattattgt
     7681 ctccgctaaa aatgaccatt tttacgggaa acattgtgct gatgctggcg ctaatggatt
     7741 cgtgagtaaa aaagaaggca tgaacaatat cattgcggct attgaagctg caaaaaatgg
     7801 ctactgctat ttccccttct ctctcaaccg gtttgttgga agtttaacgt ccgaccagca
     7861 aaaactcgac tccttatcga aacaagaaat tagtgtcatg cggtatattc ttgatggcaa
     7921 ggataataat gacattgctg aaaaaatgtt catcagcaac aaaactgtca gcacttataa
     7981 aagtcgcctg atggaaaaat tagaatgtaa atcactgatg gatctttaca cattcgcgca
     8041 acgtaacaaa atcggctaat cacatgaagt ttttacccta tttttttctt ctctgttgtg
     8101 gtctttggtc gaccataagt ttcgcagacg aagattacat cgaatatcgt ggcatcagta
     8161 gtaacaaccg tgtcacactt gatccagtac gtctgagcaa caaggaatta cgttggttag
     8221 cgagcaaaaa aaatcttgtg attgcagtac acaagtccca aacggctacc ttgttgcata
     8281 ccgattcgca gcaacaggtt cgtggtatta atgctgatta tttaaatctt ttaaaaagag
     8341 cgttaaatat caaattaaca ctccgggaat acgccgatca tcaaaaagca atggacgcgc
     8401 ttgcagaagg tgaagtcgat atagtgttat cacatttagt tacttcgccg cctcttaata
     8461 gtgacattgc tgcaaccaaa ccattgataa ttacctttcc ggcgctggta accacccttc
     8521 acgactcaat gcgaccgctt acctcaccaa aaccagtaaa tattgctcgg gtagcaaatt
     8581 accccccaga cgaggtaatt catcaatcgt ttccaaaagc aacaattatc tcttttacaa
     8641 atttatatca ggcattagca tccgtatcag ccggacagaa tgattacttt attggtagta
     8701 acatcattac cagcagtatg atttcccgct atttcactca ctccttaaat gtagtgaaat
     8761 attataactc gccgcgtcaa tataattttt tcttgaccag aaaagaatcc gtcattctta
     8821 atgaagtact caatagattt gttgatgctt taacaaatga agttcgctat gaagtttcac
     8881 aaaattggct tgatacagga aacctggcct ttctgaacaa accattagaa ctcactgaac
     8941 atgaaaaaca gtggattaag caacatcccg atttaaaggt gctggaaaat ccttactctc
     9001 ctccctattc tatgacggat gaaaatggct cggttcgggg cgttatgggg gatattctta
     9061 atattattac cttgcaaaca ggtttaaatt tttctccgat caccgtttca cacaatatcc
     9121 atgccggaac acagcttaac cccggtggat gggatataat acctggcgct atttatagtg
     9181 aagatcgaga aaataatgtt tcatttgcag aagctttcat aacaacgcca tacgtttttg
     9241 tcatgcaaaa agcacctgac agtgaacaaa cattaaaaaa aggaatgaaa gttgccattc
     9301 catattatta tgagctgcat tcgcaattaa aagagatgta tccggaggtt gaatggatac
     9361 aggtcgataa tgccagcgct gcatttcaca aggttaagga aggtgaactt gatgctctgg
     9421 tcgcgacaca gttaaattcg cgttacatga tcgatcatta ctatcctaat gaactttatc
     9481 attttcttat tcctggcgtt ccgaatgcat cgctttcgtt cgcttttcct cgcggagaac
     9541 cggaacttaa ggatattatt aataaagcac tgaatgcaat tcccccaagc gaagttctgc
     9601 gcctgacgga aaaatggatt aaaatgccca atgtgaccat tgacacatgg gacctatata
     9661 gcgagcaatt ttatattgtt acgacattat ccgttttatt agttggcagt agccttttat
     9721 ggggattcta cctgttacgc tcagttcgtc gtcgtaaagt cattcagggt gatttagaaa
     9781 accaaatatc attccgaaaa gcactctcgg attccttacc gaatccaact tatgttgtaa
     9841 actggcaagg taatgtcatt agtcataata gtgcttttga acattatttc actgcggatt
     9901 actacaaaaa tgcaatgtta ccattagaaa acagtgactc accctttaaa gatgtttttt
     9961 ctaatgcgca tgaagtcaca gcagaaacga aagaaaatcg aacaatatac acacaggtat
    10021 ttgaaattga taatggcatc gagaaaagat gcattaatca ctggcataca ttatgcaatc
    10081 ttcctgcaag tgacaatgca gtatatattt gtggttggca agatattact gaaacgcgtg
    10141 atctaattaa tgcactcgag gtagaaaaaa ataaagcgat aaaggctacc gtagcaaaaa
    10201 gtcagtttct ggcaacgatg agtcacgaaa taagaacacc aataagctct attatgggct
    10261 tcctggaact tctgtcgggt tctggtctta gcaaggagca acgggtggag gcgatttcac
    10321 ttgcctacgc caccggacaa tcactcctcg gcttaattgg tgaaatcctt gatgtcgaca
    10381 aaattgaatc gggtaactat caacttcaac cacaatgggt cgatatccct actttagtcc
    10441 agaacacttg tcactctttc ggtgcgattg ctgcaagcaa atcgatcgca ttaagttgca
    10501 gcagtacatt tcctgatcat tacctggtta agatcgaccc tcaggcgttt aagcaggtct
    10561 tatcaaattt actgagcaat gctctcaaat ttaccaccga gggggcagta aaaattacga
    10621 cctccctggt tcacattgat gacaaccacg ctgttatcaa aatgacgatt atggattctg
    10681 gaagtggatt atcgcaggaa gaacaacaac aactctttaa acgctacagc caaacaagtg
    10741 caggtcgcca gcaaaca
//
LOCUS       DAZGLJ010000154        10696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000154 DAZGLJ010000000
VERSION     DAZGLJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.154"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            52..618
                     /gene="yceJ"
                     /locus_tag="ACHK49_003488"
     CDS             52..618
                     /gene="yceJ"
                     /locus_tag="ACHK49_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO9264073.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            622..1197
                     /gene="yceI"
                     /locus_tag="ACHK49_003489"
     CDS             622..1197
                     /gene="yceI"
                     /locus_tag="ACHK49_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO9264074.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1239..2291)
                     /gene="trhO"
                     /locus_tag="ACHK49_003490"
     CDS             complement(1239..2291)
                     /gene="trhO"
                     /locus_tag="ACHK49_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO9264075.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2516..3436
                     /gene="lpxL"
                     /locus_tag="ACHK49_003491"
     CDS             2516..3436
                     /gene="lpxL"
                     /locus_tag="ACHK49_003491"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO9264076.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPQQYMWLHRRFKTRPEGVPSRY"
     gene            3608..4834
                     /gene="mdtG"
                     /locus_tag="ACHK49_003492"
     CDS             3608..4834
                     /gene="mdtG"
                     /locus_tag="ACHK49_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO9264077.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4917..5291
                     /gene="msyB"
                     /locus_tag="ACHK49_003493"
     CDS             4917..5291
                     /gene="msyB"
                     /locus_tag="ACHK49_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO9264078.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5292..5519)
                     /gene="yceK"
                     /locus_tag="ACHK49_003494"
     CDS             complement(5292..5519)
                     /gene="yceK"
                     /locus_tag="ACHK49_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO9264079.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5692..8235)
                     /gene="mdoH"
                     /locus_tag="ACHK49_003495"
     CDS             complement(5692..8235)
                     /gene="mdoH"
                     /locus_tag="ACHK49_003495"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO9264080.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8228..9763)
                     /gene="mdoG"
                     /locus_tag="ACHK49_003496"
     CDS             complement(8228..9763)
                     /gene="mdoG"
                     /locus_tag="ACHK49_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO9264081.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10157..>10696
                     /locus_tag="ACHK49_003497"
     CDS             10157..>10696
                     /locus_tag="ACHK49_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO9264082.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLS"
BASE COUNT         2639 a         2793 c         2626 g         2638 t
ORIGIN      
        1 ttctccgaca cgccattttc tttctctcgc aaattcgtat tggatgcact tatgtcattc
       61 acaaataccc ctgaacgcta tggtgttata tctgcggcct tccattggct aagcgcaatt
      121 attgtctacg gcatgtttgc tttaggtctg tggatggtca cgctcagtta ttacgatggc
      181 tggtatcaca aagcacccga actgcataaa agcatcggta ttttgttaat gatggggctg
      241 gttattcgcg ttctgtggcg tgtcatatct ccaccgccgg gtccgctgcc gagttattcg
      301 cccatgactc gtcttgccgc aaaggctgga catctcgcat tgtacctatt gctgttcgcc
      361 atcggcatca gcggctatct gatctcaact gccgatggta aaccgatcag cgtttttggc
      421 tggtttgacg tccccgcgac ccttgccgac gctggcgcac aggcagactt tgccggtgcc
      481 ctgcattttt ggctcgcgtg gagcgtcgta gtactgtccg ttatgcacgg atttatggcc
      541 ctgaagcatc atttcatcga taaagacgac actctgaagc gcatgctggg aaagtcgtca
      601 tctgactatg gagtataaaa aatgaaaaaa agcctgcttg gtttaacctt cgcgtccctg
      661 atgttctctg ccggttcagc ggttgccgcc gattacaaaa ttgacaaaga aggtcagcac
      721 gcctttgtta acttccgcat ccagcatctt ggctatagct ggttatatgg cacctttaaa
      781 gatttcgacg gtacttttac ctttgacgaa aaaaatccgg ctgccgataa agtgaatgtg
      841 acaattaaca ccaccagcgt cgatactaat cacgccgaac gcgataaaca tcttcgcagt
      901 gcagatttcc tcaataccgc aaaatatcca caggcaacat tcacctccac cagcgtgaag
      961 aaagacggtg acgaactgga tattaccggc gatctgacgc tgaatggcgt aaccaaaccc
     1021 gtcacgctgg aagcgaaatt aattggtcag ggcgacgacc catggggtgg taaacgtgca
     1081 ggcttcgagg ccgaaggcaa aattaagctc aaggacttca atatcaagac agatttaggt
     1141 ccagcttctc aggaagtgga tctgattatt tcagtggaag gcgtacagca gaagtaagcg
     1201 tgaaatgttg ccggatggca cgcatccggc aatgatattt attccgttgg atcaggaatg
     1261 cccagtgttg tattcagacg tccacgagac ttattaaaga tcttattgcc attttcacgt
     1321 cctgcccgac ggcgtcgctg ttcttctggc ggtaacgcgc tttcttcgca gcaaatctca
     1381 ctacaacaac ctttgtattt ttccgcacat actggacact gaataaacag caggtggcag
     1441 ccatcatttt tacagttggt atggctgtcg cacggcgcgc cgcactggtg gcaatgcgcg
     1501 ataatctcat ccgatatacg ttcgcccatc cgctcgtcaa aaacaaaatt tttgccaata
     1561 aaacgcaccg gcaagccctg ctcgcgcgcc ttacgggcgt attcaataat tccgccctcg
     1621 atatgccaga ctttgttgaa tccgttatgt ttcatccagg cactggcctt ttcacaacga
     1681 atgccgccgg tgcagtacat gacgattttt ttatctttat gtgcctgcat catctcgact
     1741 gcttttggca gctgctcacg gaaggtatct gccggaattt cgagcgcgtt ttcaaagtgc
     1801 cccacttcat actcatagtg gttacgcatg tcgataaata gtgcatcggg atcgtcaagc
     1861 atggcgttca cttccgccgc ttgcagatac tcgccaacat tgctggcatc aaagtgagga
     1921 tcgtcaatac cgtcggcaac aatgcgatcg cgtaccttca tgcgcagtac ccagaaagat
     1981 ttcccgtcat catccaacgc gatattcagg cgtaaaccct ctaaagccgg gtcgaaggca
     2041 tagagctgcg cgcgaaatgt ttcaacattg ctcgcaggta cgctgatttg cgcgttaatg
     2101 ccctcatgcg ccagatacac tcgcccaaaa acattcagcg cggtaaacag ctgatataaa
     2161 gcgtcacggg tcgccttagg atcggcgatg tggaaatact tgtaaaacga aatggtggtt
     2221 cgcggttcgc tctcagccaa cattttggct tttagcgcgt cgttggaaat gcggttgtgt
     2281 aacactggca tggtgtacgg ttcctgcgag atgggaaagt aaaaatccgc ggcatgatat
     2341 agcaattatc gataattaac atccacacat tttacgctac atttgcgcat taaaaattat
     2401 ttgttattta caagtgcggc aatttcgccc agtcttcagc cacaaatttt ggttgcgggc
     2461 gaaaaaatgc gacaatacat acaattgccc gaataggttg aaaaacagga ttgatatgac
     2521 gaatctaccc aagttctcca ccgcactgct tcatccgcgt tattggttaa cctggttggg
     2581 tattggcgta ctttggttag tcgtgcaatt gccctacccg gttatctacc gcctcggttg
     2641 tggattagga aaactggcgt tacgttttat gaaacgacgc gcaaaaattg tgcatcgcaa
     2701 cctggaactg tgcttcccgg aaatgagcga acaagaacgc cgtaaaatgg tggtgaagaa
     2761 tttcgaatcc gttggcatgg gcctgatgga aaccggcatg gcgtggttct ggccggaccg
     2821 ccgaatcgcc cgctggacgg aagtgatcgg catggaacac attcgtgacg tgcaggcgca
     2881 aaaacgcggc atcctgttag ttggcatcca ttttctgaca ctggagctgg gtgcgcggca
     2941 gtttggtatg caggaaccgg ggattggcgt ttaccgcccg aacgataatc cactgattga
     3001 ctggctacaa acctggggcc gtttgcgctc aaataaatcg atgctcgacc gcaaagattt
     3061 aaaaggcatg attaaagccc tgaaaaaagg cgaagtggtc tggtacgcac cggatcatga
     3121 ttacggcccg cgctcaagcg ttttcgtccc gttatttgcc gttgagcagg ctgcgaccac
     3181 gaccggaacc tggatgctgg cacggatgtc cggcgcatgt ctggtgccct tcgttccacg
     3241 ccgtaagcca gatggcaaag ggtatcaatt gattatgctg ccgccagagt gttctccgcc
     3301 actggatgat gccgaaacta ccgccgcgtg gatgaacaaa gtggtcgaaa aatgcatcat
     3361 gatggcacca cagcagtata tgtggttaca ccgtcgcttt aaaacacgcc cggaaggcgt
     3421 tccttcacgc tattaaatct cccctgccgg atgcttcaga atggcatccg gcattaccac
     3481 agcaaatccc cctgatttag cgataaaagc tctctggatt gcgccccctg gaagtcgagc
     3541 gcataattag tgtgcttatc ttttcttctt atgttcaccg cgcctggcgc accaacagcg
     3601 gattgctatg tcaccctgtg aaaatgacac ccctataaac tggaaacgaa acctgatcgt
     3661 cgcctggcta ggctgttttc ttaccggtgc cgccttcagt ctggtaatgc ccttcttacc
     3721 cctctacgtt gagcagcttg gcgttaccgg tcactccgcc ctgaatatgt ggtccggtat
     3781 tgtcttcagc attacatttt tattttcggc catcgcctca ccgttttggg gtggactcgc
     3841 cgaccgtaaa ggccgaaaac tcatgctatt acgctctgcc ctcggcatgg gcatcgtgat
     3901 ggtgttgatg ggactggcac aaaatatctg gcagtttttg atcctacggg cgcttcttgg
     3961 gttacttggc ggatttgtcc ccaacgctaa tgctcttatc gccacacaag taccgcgtaa
     4021 taaaagcggc tgggcgctgg gtacgctctc cacaggcggc gttagtggtg cgttgctcgg
     4081 cccaatggct ggcggcctgc tcgccgatag ctacggctta cgtccggtat tctttattac
     4141 cgccagtgtg ctcatactct gctttttcgt caccctgttc tgcatcagag aaaaattcca
     4201 gccggtcagc aaaaaagaga tgctgcacat gcgggaagtg gtgacatcac ttaaaaaccc
     4261 gaaactggta ctcagcctgt ttgtcaccac gttaatcatc caggtggcga caggctcaat
     4321 tgcccccatt ctgacgctgt atgtccgcga actggcgggt aacgtcagta acgtcgcctt
     4381 tatcagtggc atgatcgcct cggtgccagg cgtggcggct ctgctaagcg caccacgact
     4441 cggcaaactt ggcgatcgaa tcggacccga aaagatcctg attacagcgc tgatcttttc
     4501 tgtactgctg ttgatcccaa tgtcttacgt tcagacgcca ttgcaacttg ggattttacg
     4561 ttttttgctc ggtgccgccg atggtgcact actccccgcc gtacagacac tgttggttta
     4621 caactcgagc aaccagatcg ccgggcgtat cttcagctat aaccaatcgt ttcgtgatat
     4681 tggcaacgtt accggaccat tgatgggagc agcgatttca gcgaactacg gtttcagagc
     4741 ggtatttctc gtcaccgctg gcgtagtgtt attcaacgca gtctattcat ggaacagtct
     4801 acgtcgtcgt cgaatacccc aggtatcgaa ctgatttttc gcctttcata cttgcaaaag
     4861 cggagaatca gctatccttt tccctgaaac ctcatcaact caaagggaga atcgtgatga
     4921 ccatgtacgc aacgcttgaa gaagccattg acgctgcacg cgaagaattt cttgcagaca
     4981 accccggcat cgacgccgaa gatgcgaatg tgcaacagtt caatgcccaa aaatacgttt
     5041 tgcaggacgg cgacatcatg tggcaagttg agttttttgc cgacgaaggg gaagaaggtg
     5101 aatgtttacc tatgcttagc ggtgaagccg cgcaaagtgt ttttgatggc gactatgatg
     5161 agatagagat acgccaggag tggcaggaag agaatacatt acatgaatgg gacgaggggg
     5221 aatttcagct tgagccaccg ctggataccg aggaaggacg cgcagcagct gatgagtggg
     5281 atgaacgtta atcactcata cgggccatga tgaatgtcga tcggcagcag taaagtatca
     5341 aaaaccagag agaatggcag atcaaggatc gtgacataac gccaggcgga gtcacgcaca
     5401 tcccattgca caccgggata atattggttg ccatgcccct gccccggtat agtgcgacta
     5461 ataatgctgc cacagccact cagcaaggtc accatgatgc tcaccacaat taaacgcatt
     5521 tgatacctct ttcgtaaaaa aataccggca taacgccggt atttttattg gctcgttgtt
     5581 tttgccggat gcggcgtgaa cgccttatcc gacccacaaa atcttgcaaa atcaataaat
     5641 tgcaggaacg atgtaggcct gataagcgta gcgcatcagg caactacgtt tttattgcga
     5701 agccgcatcc ggtttacgca atgccagtgg attgagcttt atcccttcgt aataactcac
     5761 ccatgaagaa tatctctccg gggaattcca gacgcggaaa tgcagacggg ccatcgtcac
     5821 cggatcgctt agcagcacca ggcgacgatc gcgattcagc ttctctggcg tctcgttcag
     5881 cgcctgttca acgtggcggt cacgggcgat ttccagcacc ttactggcgc ggtgacgcgc
     5941 ggtcgccatt gcggttgcca gagcgttaaa tgacgggtta aacactgcgt gcataaagcc
     6001 atcatcaagg gagcgttgac gattcatctc aaggaaccga tcggtatcaa ccagcacctg
     6061 cggcggcgaa tactcttccg ggatcaggaa cagtttccag cgtttggtgc gcagaccaac
     6121 ggtggcacgg ctggaaatca ccgaaacaaa cggtgacagg atcaacgaga agacaatcgg
     6181 tgccagccag aacaggaaac gcagatccaa ccacgccata ccaacagccc acactaaccc
     6241 taacagcagc tgtgagccgt ggcgtttgaa cgcttcaccc caggaagtgg agtcatcatc
     6301 acgctgcggt gaattccaca ccacttccca gccaaggaac gcgctgacga cgaagaccgt
     6361 atggaacagc atgcgtaccg gagccagcag cacggaaaac agcacttcca gcagcaacga
     6421 taatgtaacg cgccagaagc cgccgtattc tttcgttcct ttgcaccaga taagcaaaat
     6481 gctcaataac ttcggcagga acaacagcac catggtcgaa gcaaaaagtg caatcgccag
     6541 ctcaggacgc cactgcggcc acaccgggaa caactgccgt ggttgcagga agtattgcgg
     6601 ttcggtcaac gcatgtacta cctgcaatgc agtagagagc gcgaggaaca taaaccacag
     6661 cggagcggag agataagaca tcacgcccgt caggaatacc gcacggtgaa ccgggtgcat
     6721 acccttcacc aggaacagac ggaagttcat caggttaccg tggcaccagc ggcggtcacg
     6781 ttttagctca tcaagcaagt taggcggcaa ttcttcataa gaacccggga gatcgtaagc
     6841 aatccagacc ccccaacctg cacggcgcat caacgccgct tccacgaagt catgtgacag
     6901 gattgaaccg gcaaaggaac cttcgcccgg cagcggagcc agtgcgcagt gctcgataaa
     6961 cggtttcacg cggataatcg cgttatgtcc ccagtagtgc gactcgccaa gttgccagaa
     7021 gtgcaaaccg gctgtaaaca gtggcccata cacgcgggtc gcgaactgct gacagcgcgc
     7081 atacagcgta tccataccgg acgctttcgg cgacgactga atgatcccgg cgttcgggtt
     7141 ggcttccatc aggcgcacca ggccgcataa acaatcaccg gtcattaccg agtcagcatc
     7201 cagcaccacc atgtagctgt actggctgcc ccagcgacgg cagaagtcat cgatattacc
     7261 gcttttacgc ttcacgcggc gacggcggcg gcgatagaaa atctgacctt cgccaccgac
     7321 ttcagcgata agctccatcc aggctttttg ctctgcgacg cagatatccg ggttataact
     7381 gtcactaaga atgtagacat caaagtgttt ggcattcccg gtggctttta ctgattccca
     7441 cgttgcacgc aggccagcaa aaacacggtt cacgtcttcg ttacagatag gcatgatcaa
     7501 cgccgtgcga tgctccgggt ttaatggttc atcgccaact gttgacgcag atatactgta
     7561 tttatcgcga ccaataagca gttgcaggaa gcccattaac gccgtccaga atccggcgga
     7621 cacccaacag aacagtaccg caaagaggat caggataccg gtttgcagca tataaggcag
     7681 aagctgcata aaggaaaccc acaaatcctg accaaccata tccataggat taatcagcgc
     7741 ccaaccctga taaggaagaa tggtcttcat ataccaggtc gcgacgacag tttgcgcgag
     7801 cgtcaggatc aacagaatgt aacggcggat ggtaccgacg gtacgccact tttgctcgct
     7861 ctcctgctct tctttggtca aacgagccag atagcgcggc gtgacatcgc gtccacgcag
     7921 gcgatcccag aaacggccta ccgggttggt acgccacggg tcgggaaaca tcgaggagcg
     7981 ttttgcttct ggcatcgcct tcagctgatc gcgcccttcg tcgtctttaa ttaactgtcc
     8041 atcagcaagt gaatctggcc aggcttgttc cagacgcgcc tttaccgagc cttgcgggga
     8101 atcatcctcc cgcgcccagg tgcggtgttc ggcatccagc gcctgatgaa cggcgcggat
     8161 atcagtcttc ggcaatgccg ctttctcgct tgcggcgatg ggcattgcgt caatgtactc
     8221 agttgtctta ttcattggca ggtaactggt agctccaggt ttcactcaac gtctgatcgg
     8281 cattcaccag cgcagcacgc atttcagtgg ttttcttggc atctttcact ttcacacgca
     8341 tcaccagacg ccagcctttg gtaaccgggt tatagcgcac cgtgctttca actatctcac
     8401 cattatcacc aatgctggtt tgcgctgtga ccggggtatc ctctggcagt tttttcatct
     8461 ctgcgccggt aaaatcgacc acaaaggcga tagtaccgtc aggctggcga atcaggttcg
     8521 actgcttcac atcccccgtt gaacgacgcg tttgttgcac ccatgcgtta tctggcgcat
     8581 gcagtttgtc ttcatcacgg ctgaaggtga tggtgtattt aaagttcatc tctttacccg
     8641 gctccggcag ctgatccggc gtccagtaag cgacgatgtt atcgttggtt tcatcgttgg
     8701 ttggaatttc caccagctca acgctgcctt taccccactc ccctttcgga gtcacccatg
     8761 cgcttggacg aagatcgtaa cgatcatcga gatcttcaaa gcgggagaaa tcacgaccgc
     8821 gctgcaacag accgaagcct tgcgggttct ccatcgagaa gctgctgacc gctaaatgtt
     8881 tcgggttatt caacggacgc cagatccatt cgccattacc ggcatggata gacagaccgt
     8941 tagagtcgtg caactccgga cgatagttat ttgcaggcga cggttggttc ggcccaaaca
     9001 ggaacatact ggttaacggt gcaaccccca gtttgccgac tttatcgcgc agatagattt
     9061 tcgactgcac atccacaacc gtgtcacgcc ctggcataac cacgaatttg taagcacctg
     9121 tcgcgcgcgg cgagtcaagc aatgcataaa tggttaaacg tttatcagtc ggttttggac
     9181 gctcgatcca gaactctttg aagcgtggaa attcttcacc cgatggcaag gcggtatcaa
     9241 ttgccaggcc gcgggcagaa aggccataaa cctgacctgc accaatcacg cggaaatagc
     9301 tggccccgag catgctgacg atttcatcgt ttttatcttt gctgttgatc gggtaaagca
     9361 ctttaaaacc ggcaaaacca aggtctttta ccgtgtcttt gtcatgctga acatcgccga
     9421 aagtgaaata atccgggctg tatttgattc gtttgactgc ggtggcagtc acttcattta
     9481 ttttgaccgg ggtatcgaag tacataccct gatggtagaa ctcgagtttg aatggggtct
     9541 tcagattgtt ccagtacgct ttgtcatgat taaactggat ctgctgatag tccgcgtatt
     9601 tcatatcgcg gaaaacggag ggcaagttgc ttttgggcgc ctcatagcct ttcccggcta
     9661 aggattgagc ttgctttgcg acatcatcaa tactgaaagc ccagcttgaa gatgtataca
     9721 gggttaacat tactgcagca ctcaaccaac gcattttcat catttgtagt ttatgtttca
     9781 taattagtaa gcacttcccc ctttgtgtgc ttatatcgat ccgatctatt ttaatggata
     9841 accagccatt ccgacaacag aatcccggta tcgttcagca cgctggctca tggattaagc
     9901 acctgattgc aaccgttttc actttccgtg cttctcctaa agacagaagc atcgacttcg
     9961 tgtagggttt gccagcaatt ttatttatca accgtcttac agacgcttcc attctggtta
    10021 gtggtagcga tgatgctatg cataaaaacc ttgaagcctg cgcctggcgc ggtcaggtcg
    10081 gtgtcgctat ggcaacaagc ctaaaggcat aacccgacag ggataagacg aaaaatccga
    10141 gattagttaa ccatatatga atccagtacc cgcgcaacgt gaatatttcc tcgactccat
    10201 ccgcgcctgg ctgatgttgt tagggatccc ttttcatatt tctttaatct attcgagcca
    10261 tacatggcat gtgaatagcg ccgaaccatc attgtggctg acccttttta atgacttcat
    10321 ccactcgttc cgcatgcagg tatttttcgt tatatccggc tacttttcct acatgctttt
    10381 tttacgctat cccttgaaaa aatggtggaa agtacgtgtc gaacgtgtag gtatcccgat
    10441 gttaacagcc atccccctac tgacattacc gcaatttatt atgctgcaat atgtcaaagg
    10501 aaaagcggaa agttggcctg ggctgtcatt gtatgacaaa tataatacgt tggcctggga
    10561 attaatatca cacctgtggt ttttactggt gttagtagtc atgacgacgc tgtgcgtatg
    10621 gatatttaag cgcatcagaa ataatttaga aaattctgat aaaacgaata aaaaattctc
    10681 gatggtaaaa ctatcg
//
LOCUS       DAZGLJ010000155        10633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000155 DAZGLJ010000000
VERSION     DAZGLJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.155"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            34..759
                     /locus_tag="ACHK49_003498"
     CDS             34..759
                     /locus_tag="ACHK49_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9264083.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            717..1394
                     /gene="bglJ"
                     /locus_tag="ACHK49_003499"
     CDS             717..1394
                     /gene="bglJ"
                     /locus_tag="ACHK49_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO9264084.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMTRISFAAVIFSFSAMRSERREGLSCLTELAIKLPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1432..2220)
                     /gene="fhuF"
                     /locus_tag="ACHK49_003500"
     CDS             complement(1432..2220)
                     /gene="fhuF"
                     /locus_tag="ACHK49_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO9264085.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLRAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHVEFHETGRAACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2361..2597
                     /locus_tag="ACHK49_003501"
     CDS             2361..2597
                     /locus_tag="ACHK49_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO9264086.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLILTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMLMNLNQG"
     gene            complement(2636..2722)
                     /locus_tag="ACHK49_003502"
     tRNA            complement(2636..2722)
                     /locus_tag="ACHK49_003502"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2686..2688),aa:Leu,seq:cag)
     gene            complement(3023..4054)
                     /gene="rsmC"
                     /locus_tag="ACHK49_003503"
     CDS             complement(3023..4054)
                     /gene="rsmC"
                     /locus_tag="ACHK49_003503"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO9264087.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVAKADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4157..4570
                     /gene="holD"
                     /locus_tag="ACHK49_003504"
     CDS             4157..4570
                     /gene="holD"
                     /locus_tag="ACHK49_003504"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO9264088.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTDLRANPTARAALWQQICTYEHDFFPRND"
     gene            4539..4985
                     /gene="rimI"
                     /locus_tag="ACHK49_003505"
     CDS             4539..4985
                     /gene="rimI"
                     /locus_tag="ACHK49_003505"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO9264089.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5000..5677
                     /gene="yjjG"
                     /locus_tag="ACHK49_003506"
     CDS             5000..5677
                     /gene="yjjG"
                     /locus_tag="ACHK49_003506"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO9264090.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            5768..7357
                     /gene="prfC"
                     /locus_tag="ACHK49_003507"
     CDS             5768..7357
                     /gene="prfC"
                     /locus_tag="ACHK49_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO9264091.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            7750..8355
                     /gene="osmY"
                     /locus_tag="ACHK49_003508"
     CDS             7750..8355
                     /gene="osmY"
                     /locus_tag="ACHK49_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO9264092.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8482..8643
                     /locus_tag="ACHK49_003509"
     CDS             8482..8643
                     /locus_tag="ACHK49_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO9264093.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            8765..9838
                     /locus_tag="ACHK49_003510"
     CDS             8765..9838
                     /locus_tag="ACHK49_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO9264094.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            9835..10617
                     /locus_tag="ACHK49_003511"
     CDS             9835..10617
                     /locus_tag="ACHK49_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO9264095.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         2602 a         2657 c         2837 g         2537 t
ORIGIN      
        1 tcataatttt tagacagtgg atgtggaggg aatatgttgc caggatgctg caaaaatgga
       61 attgttatca gtaaaatacc tgttatgcaa gcagggttaa aagaggtcat gaggactcac
      121 ttccctgaat atgaaataat atccagcgcc tctgcggagg accttacctt attacaatta
      181 cgccgttccg gattagtcat tgctgattta gccggtgaaa gtgaagatcc acgttctgtt
      241 tgtgaacatt attattcttt aatctcacaa tatcgggaaa ttcactgggt tttcatggtg
      301 tcacgctcct ggtattccca ggcagtagaa ctgctcatgt gccctacggc gacgttattg
      361 tctgatgttg aacccattga gaatctggtc aagaccgtac gttccggcaa tacgcacgca
      421 gagcgtatca gcgccatgct gacctctccg gcaatgactg aaactcatga ttttagctat
      481 cgctccgtca ttctcactct ttcagagcgc aaggtactgc ggctattagg taaaggatgg
      541 ggcatcaacc agatagcttc attgcttaag aaaagtaata aaactatcag cgcccaaaaa
      601 aacagtgcga tgcgtcgact ggcaattcac agcaacgctg aaatgtatgc atggataaat
      661 agcgcgcagg gtgcaagaga acttaacttg ccttctgttt atggagatgc cgcagaatgg
      721 aacacagccg aattaagaag agaaatgtcg cactcataga aaaatgcgtc atgagtagta
      781 tcggtattga gagtttattc agaaagtttg cgggtaaccc ttataagctc catacctata
      841 ccagtcagga gtcatttcag gatgccatga cgcggatctc gtttgcggcg gtcatttttt
      901 ctttttctgc catgagaagt gagcgcagag agggattatc ttgcctgact gaactggcga
      961 ttaagcttcc gcgtacccgg cgtttagtta ttgcggatga tgatattgaa gctcggctga
     1021 ttggttcatt gtcgccatca ccgctggacg gtgtattaag taaagcgtca acgctggaga
     1081 tttttcatca ggaactcttt ttgtcattaa atggtgtacg tcaggcgacc gaccgactga
     1141 acaatcagtg gtacattaac caaagccgga cgctaagccc gacggagaga gaaatattgc
     1201 gctttatgtc gcgtggttac tcaatgacac aaattgccga gcagcttaaa cgtaatatca
     1261 aaacgatccg tgcacataaa tttaatgtga tgtcgaaact gggcgtcagt tctgatgcag
     1321 ggttgttgga ggccgcagat attctgttat gtatgcggca ttgcgaagca agtaatgtgt
     1381 tgcatcccta ttaatccgca tgatgccggg tttacttccc cggcagtgct ttcatttcag
     1441 cgtacaatcg ccacattgct gcacatccgg taagcgataa cgctggcagc aagtgcggcg
     1501 caccagcagg ccgtcgcgca gtaccacggt acgccagagt ggattatctt caccggtagt
     1561 aagcgttttc tcaaaaaaga gggcatggcg cagcgattca accgtcgcct cgccgagcag
     1621 ctgtttcatc tcagtcagat accagttgat caaataaccg gtattactcc agataagttt
     1681 gccgttgatc tctcccgtcg cttctagtgc ctgcacaacc ggaaccagcg cctggctgat
     1741 taacgtttcc attcgctgct gcggcgaatg tggtgttgcg tttttatctt cacacacatc
     1801 gacccagaaa caggcggcac ggccggtttc gtgaaactca acgtggaaat gttccggcga
     1861 cacatctaac gctttttcct gcgtcagcag cgccagcatt aatggtggca ccatcaggcc
     1921 gatataccat tgcgcccata gtgagatcag cggcttgttc tcgcggatca tcgttggttg
     1981 gttgcgatag atatgatcgg aatagaccgc cagtagagaa cttagcgcat tcggtgatga
     2041 ccattgcgcc agcgtcatgg cgcgaagcgg ggcgggttca tccagacgga taaactccag
     2101 caaatgttca cgatgttcgg cgatcgtcgc ccgtacggct tgtgcaagcc cagcatcctg
     2161 cggctggaga tgcgttcgcc agatgacatc ttcatagagc ggtgcggaac gataggccat
     2221 aatcgggata gtaatctaaa tgataatgat tgctaatcct agcgataggt ttacccgata
     2281 gcaagggatt tctctggctt gcaaatgata aaaattatca tatgatattg gttatcatta
     2341 tcaatgaaag agatgaaacc atgttgcaac gtacgctggg cagtggctgg ggagtgttgc
     2401 tgccgggatt gctgattgca gggctgatgt atgcggattt atcgccggat caatggcgga
     2461 ttgtcattct gatgggattg atattgacgc cggtgatgct gtatcacaaa cagttacggc
     2521 attacgtttt gctaccatcg tgcctggcgc ttattgctgg catcatgctg atgctaatga
     2581 atttgaatca gggatgaaaa atcaaggaag aaacaagaaa ggaagtaaag ataattggtg
     2641 cgaggggggg gacttgaacc cccacgtccg taaggacact aacacctgaa gctagcgcgt
     2701 ctaccaattc cgccaccttc gcataccatc aattcttaaa aagaattgct accacggagg
     2761 cgcattctag tggttttcag cttttcgtca atagttaatt atcgccggag gtgtaattgc
     2821 tggaaaaatg tccatcagga aactaacgtg caggttttgt aggtcggata aggcgttcac
     2881 gccgcatccg acacggtatt tgggtgcaga tgccagatgc gacgcgggcg cgtcttatct
     2941 ggcctacgaa gggctaacgt gcaggttttg taggttgtat aaggcgagca cgccgcatcc
     3001 gacaaggctt gcggcgggat acttaacctt tcttcgcctg gcgggtcata atggcgcgat
     3061 acaccttgaa gcgcccggtt tgcgcgataa cttcatggaa gccaaatgtc tcatccagca
     3121 cgtccgggta aggcaggaag gcgttcgcta caattcgcag ctcgccgccg ctattaagat
     3181 gacgcaccgc gccgcgaatc agcgtttgcg ccgcatccag gctggtttgc atcccatcgt
     3241 ggaacggcgg gttggagatg atcatatcaa aacgaccttt cacctcggaa aagacgttgc
     3301 tggcaaagac ttcaccttca acaccgttgg ccgcaagcgt cgcacggctg gcttctaccg
     3361 ccggggcaga gacatcgcac aaggtgagac gaattttcgg cgaatggcgc gcaaaggcaa
     3421 ctgaaagcac acccgcgcca cagccgacat ccagcacttt acctttcgtg tgcggggtta
     3481 acgtcgagag cagcaactgg ctaccgacat ccagaccgtc gcggctaaac acgccaggca
     3541 gcgttttgac cgtcaggcca tcgacgctgt attcgcccca gaatttatcc gcatcaaata
     3601 ctggctgttt ttccagacga ccaaaataga ggccacagcg acgagcgctg tcgactttat
     3661 tcaacggcgc ataatctgcc agcatctgct cggcgctgcg cacgccgctg cggttctcgc
     3721 caacgacaaa aatatccgtc cccaccggca gcagagaaag taaattcatc aactggaact
     3781 gggcttccgg tttgttcttc ggccagtagt aaatcagcgt atcgcaatct gcgacgtcat
     3841 ccgccttggc gaccagacta aaacgggcgt tatcccccat ctggcggctt aatacctgcc
     3901 agtggtggaa ttgctgggta tgagcacggc tggccgcggt atctaaacgc gcgggcaggt
     3961 catcctgtaa gtctccggca aacagaatac ggctttgttc gaaatcatca ctgtgacgca
     4021 gcaagacttc acttgccggg gtaaatgcag acatggaatg ctcctcaatt gatactggcg
     4081 gcgattatag ccatatgttg gcgcggtatc gacgaatttg ctatatttgc gcccctgaca
     4141 acaggagcga ttcgctatga catcccgacg agactggcag ttacagcaac tgggcattac
     4201 ccagtggtcg ctgcgtcgcc ctggcgcgtt gcagggggaa attgccattg cgatcccggc
     4261 acacgtccgt ctggtgatgg tggcaaacga tcttcccgcc ctgactgatc ctttagtgag
     4321 cgatgttctg cgcgcattaa ccgtcagccc cgatcaagtg ctgcaactga cgccagaaaa
     4381 aatcgcgatg ctgccgcaag gcagtcgctg caacagctgg cggttgggta ctgacgaacc
     4441 gctatcactg gaaggcgctc aggtggcatc accggcgctc accgatttac gggcaaaccc
     4501 aacggcacgc gccgcgttat ggcaacaaat ttgcacatat gaacacgatt tcttccctcg
     4561 aaacgactga tttaccagcg gcttaccaca ttgaacaacg cgcccacgcc tttccgtgga
     4621 gtgaaaaaac ctttgccagc aaccagggcg agcgttatct caactttcag ttaacgcaaa
     4681 acggcaaaat ggcggcgttt gcgattacgc aagtggtgct ggatgaagct acattgttca
     4741 atattgcggt cgatcctgac tatcagcgtc agggattggg aagggcgctg ctggaacatc
     4801 tgatcgacga actggaaaaa cgcggcgtgg cgacgctctg gctggaagtc cgtgcctcaa
     4861 atgctgccgc cattgccctg tacgaaagtt taggctttaa cgaggcgacg attcgccgca
     4921 attactaccc caccacggac ggtcgcgaag acgccatcat catggcgttg ccaatcagta
     4981 tgtaagacaa ggtggaataa tgaagtggga ctggattttc tttgatgccg atgaaacgct
     5041 gtttaccttt gactcattca ccggcctgca gcggatgttt cttgattaca gcgtcacctt
     5101 taccgctgaa gattttcagg actatcaggc cgttaacaag ccactgtggg tggattatca
     5161 aaacggcgcg atcacttcat tacagcttca gcacgggcgt ttcgagagct gggccgaacg
     5221 gctgaaagtt gaagcaggct tgcttaacga tgcctttatt aatgcgatgg cggaaatctg
     5281 cacgccgctg ccgggcgcgg tttctctgct taacgccatt cgtggcaacg ccaaaatcgg
     5341 catcatcacc aacggcttta gcgccttgca gcaagtgcgt ctggaacgca cgggcctgcg
     5401 tgattacttc gatttgctgg tgatttccga agaagttggc gttgctaaac cgaataagaa
     5461 aattttcgat tatgcgctgg aacaggcggg caatcctgac cgttcacgcg tgctgatggt
     5521 tggcgacact gccgagtccg atattctcgg tggcatcaac gccgggcttg cgacctgctg
     5581 gctgaatgcg cacaatcgcg agcaaccaga aggcatcgcg cccacctgga ccgtttcttc
     5641 gttgcacgaa ctggagcagc tcctgtgtaa acactgattg cctccccccc gttgatgggt
     5701 aaaatagccg caatttttcg ttttcaacaa gcgcggcgcg atgccgctta ctcaagaaga
     5761 aagaattatg acgttgtctc cttatttgca agaggtggct aagcgccgca cttttgccat
     5821 tatttctcac ccggacgccg gtaagactac catcaccgag aaggtgctgc tgttcggaca
     5881 ggccattcag accgccggta cagtaaaagg ccgtggttcc aaccagcacg ctaagtcgga
     5941 ctggatggag atggaaaagc agcgtgggat ctccattact acgtctgtga tgcagtttcc
     6001 gtatcacgat tgcctggtta acctgctaga caccccgggg cacgaagact tctcggaaga
     6061 tacctatcgt accctgacgg cggtggactg ctgcctgatg gttatcgacg ccgcaaaagg
     6121 tgttgaagat cgtacccgta agctgatgga agttacccgt ctgcgcgaca cgccgatcct
     6181 cacctttatg aacaaacttg accgtgatat ccgcgacccg atggaactgc tcgatgaagt
     6241 tgagaacgag ctgaaaatcg gctgcgcacc gatcacctgg ccgattggct gcggcaagct
     6301 gtttaaaggc gtttaccacc tttataaaga cgaaacctat ctctatcaga gcggtaaagg
     6361 tcacaccatt caggaagtcc gcattgttaa agggctgaat aacccggatc tcgatgctgc
     6421 ggttggtgaa gatctggcgc agcagctgcg tgacgaactg gaactggtga aaggcgcgtc
     6481 taacgagttc gacaaagaat tgttccttgc gggcgaaatc actccggtat tcttcggtac
     6541 tgcgctgggt aacttcggtg tcgatcatat gctggatggc ctggtggagt gggctcctgc
     6601 gccgatgccg cgtcagactg atacccgtac cgtagaagcg agcgaagaca aatttaccgg
     6661 cttcgtattt aaaattcagg ccaacatgga cccgaaacac cgcgaccgcg tggcgtttat
     6721 gcgcgtggta tccggtaaat atgaaaaagg catgaagctg cgccaggtgc gcaccgcgaa
     6781 agacgtggtg atctctgacg cgctgacctt tatggcgggt gaccgttcgc acgttgaaga
     6841 agcgtatcct ggcgatatcc tcggcctgca caaccacggc accattcaga tcggcgacac
     6901 ctttacccag ggtgagatga tgaagttcac cggtattccg aacttcgcac cagaactgtt
     6961 ccgtcgtatc cgcctgaaag atccgctgaa gcaaaaacag ctgctcaaag ggctggtaca
     7021 gctttccgaa gagggcgcgg tgcaggtgtt ccgtccgatc tccaacaacg atctgatcgt
     7081 tggtgcagtt ggtgtgctgc agtttgatgt agtggtatcg cgcctgaaga gcgaatacaa
     7141 cgttgaagca gtatatgaat cagtcaacgt tgccaccgcc cgctgggtag aatgtgcaga
     7201 cgcgaagaaa ttcgaagagt tcaagcgtaa gaacgaaagc caactggcgc ttgatggcgg
     7261 cgataacctc gcttacatcg ctaccagcat ggtcaacctg cgcctggcgc aggaacgtta
     7321 tccggacgtt cagttccacc agacccgcga gcattaattc ttgcctccag ggcgcggtag
     7381 ccgctgcgcc ctgtcaattt cccttcctta ttagccgctt acggaatgtt cttaaaacat
     7441 tcacttttgc ttatgttttc gctgatatcc cgagcggttt caaaattgtg atctatattt
     7501 aacaaagtaa tgacatttct gacggcgtta aataccgttc aatgcgtaga tatcagtatc
     7561 taaagccgtc gattgtcatt ctaccgatat taataactga ttcagaggct gtaatggtcg
     7621 ttattcatca cccatcgctt ttgtgatggc gaccattgac ttctgtagag ggtgaagtct
     7681 ctccctattc agcaatgcaa cctcgtgttg ccaggctcaa attacgagca aacatacagg
     7741 aataaatcga tgactatgac aagactgaag atttcgaaaa ctctgctggc tgtaatgttg
     7801 acctctgccg tcgcgaccgg ctctgcctac gcggaaaaca acgcacagac taccaatgaa
     7861 agcgcagggc aaaaagtcga tagctctatg aataaagtcg gtaatttcat ggatgacagc
     7921 gccatcaccg cgaaagtgaa ggcggcgctg gtggatcatg acaacatcaa gagcaccgat
     7981 atctctgtca aaaccgatca aaaagtcgtg accctgagcg gtttcgttga aagccaggct
     8041 caggccgaag aggcagtgaa agtggcgaaa ggcgttgaag gggtgacctc tgtcagcgac
     8101 aaactgcacg ttcgcgacgc taaagaaggc tctgtgaagg gctacgcggg tgacaccgcc
     8161 accaccagtg aaatcaaagc caaactgctg gcggacgata tcgtcccttc ccgtcatgtg
     8221 aaagttgaaa ccaccgacgg cgtggttcag ctctccggta ccgtcgattc ccaggcacag
     8281 agcgaccgtg ctgaaagtat cgccaaagcg gtagatggtg tgaaaagcgt taaaaatgat
     8341 ctgaaaacta agtaattcgt cgtaattcgt cctcccgaac tttgtcggga ggcgcgatgt
     8401 gcaccacact aaaaatatcg cattgagtga ttttcacgct catatcaagc ggtgacatta
     8461 actatggtaa aggagacgct tatgtttcgt tggggcatca tatttctggt tatcgcgtta
     8521 atcgccgccg cacttgggtt tggtggtctg gccggtaccg ctgcaggcgc agctaaaatt
     8581 gtctttgtcg tcgggattat tctgttcctg gtgagtttgt tcatgggccg aaaacgaccc
     8641 tagatttcag accattaaga aaattttatc caaagccagt ccagcggact ggcttttgcg
     8701 gttttagcga ataataaatt gcgttacttt gtcattcttt cacaacaaaa acaggaaggc
     8761 agaggtgggg cagcgaatac ctgtaacgct tggtaatatt gcgccgttgt cgctaaggcc
     8821 gttccagcct ggacgaatag ctctggtgtg cgaaggcggc ggacagcgtg gaattttcac
     8881 ggctggcgtg ctggatgagt ttatgcgcgc gcagtttaat cctttcgatc tttatctcgg
     8941 cacatctgcc ggggcgcaga acctctcggc gtttatctgc aatcagcccg gttacgcgcg
     9001 caaagtcatc atgcgctata ccacaaaacg cgaatttttc gatccattgc gctttgtccg
     9061 tggaggaaat cttatcgatc tcgactggct ggtggaggcc accgcaagcc agatgccgtt
     9121 gcaaatggat accgccgcgc ggttgtttga cagcggcaaa tcgttttata tgtgcgcctg
     9181 tcgtcaggat gactacgcgc ctaattactt tttaccaacc aaacaaaact ggctggatgt
     9241 gattcgcgcc tccagtgcga tacctggctt ttatcgtagc ggagtgtcgc tggaaggcat
     9301 taactacctg gatggcggga tcagtgatgc gattccggtt aaagaggcgg caaggcaggg
     9361 cgctaaaacg ttggtcgtca tccgcactgt gccgtcacaa atgtactaca cgccgcagtg
     9421 gttcaaacgc atggaacgct ggctgggtga cagtagcctg cagccgctgg tcaatctggt
     9481 gcagcatcat gaaaccagct atcgtgacat tcagcaattt attgagaaac caccgggcaa
     9541 gctgcggata ttcgaaattt atccaccgaa accattacat agtatcgcgc ttggcagtcg
     9601 gattccggcg ctgcgtgaag actataaact tgggcgttta tgcggtcgtt atttcctcgc
     9661 cacggttggc aagctattaa ctgaaaaagc gccgcttacc cgccatctgg tgccagtggt
     9721 gacgccggaa tcgatcgtta ttccgcctgc gccagtcgcc aacgatacgc tggttgccga
     9781 agtgagcgat gctccgcagg cgaacgaccc gacatttaac aatgaggatc tggcttgatt
     9841 tgccgtttta tcgataccca ctgccatttt gatttcccgc cgtttagtgg cgatgaagag
     9901 gccagcctgc aacgcgcggc acaagcgggc gtaggcaaga tcattgttcc ggcgacagag
     9961 gcggaaaatt ttgcccgcgt gcaggcgtta gcggaaaaat atcaaccgct gtatgccgca
    10021 ttggggttgc atccggggat gttggaaaaa catagcgatg tgtctcttga tcagctacag
    10081 caggcgctgg aaaggcgtcc ggcgaaggtg gtggcggtgg gggagatcgg tctggatctc
    10141 ttcggcgacg atccgcaatt tgagaggcag cagtggttac tcgacgaaca gctgaaactg
    10201 gcgaaacgct acgatctgcc ggtgatcctt cattcccggc gtacacacga caaactggcg
    10261 atgcatctta aacgtcacga tttaccgtgc actggcgtgg ttcacggttt ttccggcagc
    10321 ctgcaacagg ccgagcggtt tgtacagctg ggctacaaaa tcggcgtagg cggtactatc
    10381 acctatccac gcgccagtaa aacccgcgat gtcatcgcaa aattaccgct ggcatcgttg
    10441 ttgctggaaa ccgacgcgcc ggatatgccg ctcaacggct ttcaggggca gcctaaccga
    10501 ccggaacagg ctgtgcgggt gtttgacgtg ctttgcgagt tgcgcccgga accagaagat
    10561 gagattgccg aagtgttgct taataatacg tatgcgttgt ttagcgttag tgggtagttt
    10621 acccttcgta ggc
//
LOCUS       DAZGLJ010000156        10593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000156 DAZGLJ010000000
VERSION     DAZGLJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.156"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            279..854
                     /gene="ssuE"
                     /locus_tag="ACHK49_003512"
     CDS             279..854
                     /gene="ssuE"
                     /locus_tag="ACHK49_003512"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO9264096.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNCLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            847..1806
                     /gene="ssuA"
                     /locus_tag="ACHK49_003513"
     CDS             847..1806
                     /gene="ssuA"
                     /locus_tag="ACHK49_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO9264097.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGAVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAKVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1803..2948
                     /gene="ssuD"
                     /locus_tag="ACHK49_003514"
     CDS             1803..2948
                     /gene="ssuD"
                     /locus_tag="ACHK49_003514"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO9264098.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2959..3750
                     /gene="ssuC"
                     /locus_tag="ACHK49_003515"
     CDS             2959..3750
                     /gene="ssuC"
                     /locus_tag="ACHK49_003515"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO9264099.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3747..4514
                     /gene="ssuB"
                     /locus_tag="ACHK49_003516"
     CDS             3747..4514
                     /gene="ssuB"
                     /locus_tag="ACHK49_003516"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO9264100.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4721..7333)
                     /gene="pepN"
                     /locus_tag="ACHK49_003517"
     CDS             complement(4721..7333)
                     /gene="pepN"
                     /locus_tag="ACHK49_003517"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO9264101.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7599..8801
                     /gene="pncB"
                     /locus_tag="ACHK49_003518"
     CDS             7599..8801
                     /gene="pncB"
                     /locus_tag="ACHK49_003518"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO9264102.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8970..10370
                     /gene="asnS"
                     /locus_tag="ACHK49_003519"
     CDS             8970..10370
                     /gene="asnS"
                     /locus_tag="ACHK49_003519"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO9264103.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2422 a         2719 c         2814 g         2638 t
ORIGIN      
        1 gccatttaaa tcaagcatac taaatccaat aagtgacaaa tctttaattt atagatttca
       61 tgaatattta aatattaact gaacgagtaa ttcattgatt caacatctaa tgactatcca
      121 acaaataaat atgcgtgata tataaagccc tttctttagt ttattttcag aaaaagatac
      181 acaaaattta tatttggcat tttcttgtct ctccgacaga ctgaccgaat tgttatcaat
      241 gttaacaaaa aaagaacaat tggttataag gagagagtat gcgtgtcatc accctggcgg
      301 gtagtcctcg ctttccttct cgctccagct ccttgctgga atatgcgcgg gaaaaactaa
      361 attgcctgga tgtagaggtt tatcactgga atctgcaaaa cttcgccccg gaagatctgc
      421 tttacgctcg tttcgatagt ccggcactca agaccttcac cgaacagctg caacaggctg
      481 atggactgat agtcgctaca cctgtgtata aagccgccta ttccggcgcg ctgaaaaccc
      541 tgctcgacct gctgccagag cgcgctttgc aaggcaaagt ggtgctaccg ctggcgacgg
      601 gcggtacggt ggctcatctg ctggcggtcg attatgccct taagccagtt ttaagtgcga
      661 tgaaagccca ggagatcctg cacggcgtgt ttgccgatga ctcacaagtt attgattacc
      721 atcacaaacc ccagttcacg ccaaatctgc aaacccgtct tgataccgcg ctagaaactt
      781 tctggcaggc attgcaccgc cgcgatgttc aggttcctga ccttctgtct ctgcgaggta
      841 atgtccatgc gtaacatcat taaactggcg ctggcgggat tgcttagcgt ctctactttt
      901 gcggttgctg cagaatcctc gcctgaagcg ttacgtatag gctatcagaa aggcagtatt
      961 ggtatggtat tggcaaaaag ccaccagtta ctggaaaaac gctatccgca atcaaaaatc
     1021 tcctgggtgg agttccccgc gggtccgcaa atgttggaag cgttaaacgt tggcagtatt
     1081 gatctcggca gtaccgggga tattccgcca atctttgccc aggctgccgg ggctgatttg
     1141 gtatatgtgg gcgtcgagcc accgaagccc aaagccgaag tgattctggt ggcagaaaac
     1201 agcccgatca aaaccgtagc cgatcttaaa ggtcacaaag ttgcctttca gaaaggttcc
     1261 agttcacaca accttttact gcgtgcactg cgtcaggccg gacttaagtt taccgatatc
     1321 caacccactt acctgacgcc cgctgatgcc cgcgccgcgt tccagcaagg taacgttgac
     1381 gcctgggcta tctgggatcc ctactactct gctgcattat tacagggcgc cgtgcgggtg
     1441 ctgaaagacg gcaccgatct caatcaaact ggatcgtttt atctggcagc tcgtccctat
     1501 gcagaaaaaa acggcgcttt tattcagggc gtactcgcaa cctttagtga ggccgatgcg
     1561 ttaacccgca gccagcgcga gcaaagcatc gctttactgg caaaaacgat gggcttaccg
     1621 gcaccggtga ttgcctctta cttagatcat cgccctccta ccaccatcaa accggttaac
     1681 gccaaggttg ccgccttaca gcagcaaacg gcagatctgt tttatgaaaa tcgtctggtg
     1741 ccgaaaaaag tcgatattcg ccagcgcatc tggcagccca ctcaactgga aggaaaacaa
     1801 ttatgagtct gaatatgttc tggtttttac cgacccatgg tgacgggcat tatcttggaa
     1861 cggaagaagg ttcacgcccg gttgatcacg gctatctgca acaaattgcg caagcggcgg
     1921 atcgtctggg ctataccggt gtgctgatcc caacggggcg atcgtgtgaa gatgcgtggc
     1981 tggtggcggc gtcgatgatc ccggtgacgc agcggctgaa gtttcttgtc gccctgcgcc
     2041 ccagcgtaac ctcacctacc gttgccgctc gccaggccgc cacgcttgac cgtctctcaa
     2101 atggtcgtgc gttgtttaat ctggtcacag gcagcgatcc acaagagctg gcaggcgacg
     2161 gcgtgttcct tgatcatagc gagcgctacg aagcctcggc ggaatttacc caggtctggc
     2221 ggcgcttatt gctgggggaa accgtcaatt tcaacggtaa acatattcat gtgcgcggag
     2281 caaaactgct cttcccgccg attcaacagc cttatcctcc gctttacttt ggcggatcgt
     2341 cagacgttgc ccaggagctg gcggcagaac aagttgatct ctacctcacc tggggcgaac
     2401 cgccagaact agtaaaagag aagatcgaac aagtgcgggc gaaagctgcc gcgcatggac
     2461 gcaaaattcg tttcggtatt cgtctgcatg tgattgttcg tgaaactaac gacgaagcgt
     2521 ggcaggccgc cgagcggtta atctcgcatc ttgatgatga aactatcgcc aaagcacagg
     2581 ccgcattcgc ccggacggat tccgtaggac aacagcgaat ggcggcgtta cataacggca
     2641 agcgcgacaa tctggagatc agccccaatt tatgggcggg cgttggctta gtgcgcggcg
     2701 gtgccggaac ggcgctggtg ggcgatggtc ctacggtcgc tgcgcgaatc aacgaatacg
     2761 ccgcgcttgg catcgacagc tttgtgcttt cgggctatcc gcatctggaa gaagcgtatc
     2821 gggtcggcga gttgctgttc ccacatctgg atgtcgccat tccggaaatt ccccagccgc
     2881 agccgctgaa tccgcaaggc gaagcggtgg cgaatgattt tatcccccgt aaagtcgcgc
     2941 aaagctaagg aagaaataat ggcaacgcca gtgaagaagt ggttattgcg cgttgccccc
     3001 tggtttttac cggtgggcat cgtggcggtg tggcaactgg cctcctcagt tggctggctg
     3061 tcgacgcgta ttttgccctc accggaaggg gtggtgacgg cgttctggac gctctccgcc
     3121 agcggcgaac tgtggcagca tctggcgatc agttcctggc gggcgctgat tggcttttca
     3181 attggcggat cgctggggtt gattttggga ctgattagcg ggctatctcg ctggggagaa
     3241 cggctgctgg atacctcaat tcagatgttg cgcaacgtac cgcatctggc gctgatcccg
     3301 ctggtgattt tgtggtttgg cattgatgaa tccgcaaaaa tctttctggt ggcgctcggc
     3361 acgctattcc ccatttatat caacacctgg catggcatcc gtaatatcga tcgcggtctg
     3421 gtggaaatgg cgcgtagcta tggattatcc ggcataccgc tgtttatcca tgtgatcctg
     3481 cctggtgccc tgccctcaat tatggtcggc gtgcgttttg cgttgggcct gatgtggctg
     3541 acgctgattg ttgccgaaac catttctgcc aattcaggca ttggttatct ggcgatgaat
     3601 gcgcgggagt ttttgcaaac ggacgtggtg gtagtcgcta ttattcttta cgccctgctc
     3661 ggcaaacttg ctgacgtcag cgcgcagctg ttagagcgcc tctggttgcg ctggaacccg
     3721 gcttatcatt tgaaggaggc cacggtatga atactgctcg tctgaaccag ggcacaccat
     3781 tgttgctcaa tgcagtaagc aaacattacg cggaaaatat cgtcctgaac caactggatt
     3841 tacatattcc ggcaggtcag tttgtggcgg tggtgggccg cagcggtggt ggcaaaagta
     3901 ccctgctgcg cctgctggca ggtctggaaa cgccaaccgc aggcgatgtg ttagcgggca
     3961 ccacaccgct ggctgagatt caagaagaca cgcgaatgat gtttcaggat gcgcgtctgc
     4021 tgccgtggaa atcggtgatt gataacgttg ggttaggcct taaaggccag tggcgcgatg
     4081 ccgcgcgtcg ggcgttggct gcagtaggac tggagaatcg cgccggggaa tggcctgccg
     4141 cactttctgg cgggcagaaa cagcgtgtgg cgctggcaag agcgttgatt catcgaccgg
     4201 gtttattgtt gcttgatgaa ccgctcgggg cgctggacgc cttaacgcga ctcgagatgc
     4261 aggatttgat tgtgtcactt tggcagcagc acggctttac cgtactgttg gtgacgcatg
     4321 atgtgagcga agcggtagca atggctgacc gggtgttgtt aattgaagag ggaaaaattg
     4381 gtctggattt gacagtggat atcccccgtc cgcgtcgatt agggtcggtg cgattagcgg
     4441 agctggaagc ggaagtattg caacgggtaa tgcggcgtgg gcattctgaa caactgattc
     4501 ggcgtcatgg ttaatatgcc ggatgtggcg cacaaaacca gcattttcaa cggactagta
     4561 ggcctgataa gacacgtcaa gcatcgcatc aggcagttgg gcacggctgc cggatgcggc
     4621 gtgaacgcct tatccagcct acaaaaccag caatttcaac ggagttgtag gcctgataag
     4681 acgcgtcaag cgtcgcatca ggcattttgg ctcaaaagta tcaagccagt gctttggtga
     4741 tcttctcgta cagatcgcca gagagatttt ccagcccttt caactgttcc agcgccgcgc
     4801 gcattttctc ctgacgtttg gcatcataac gtttcaggcg aatcagcggt tcaatcagac
     4861 gtgaagctac ctgcgggtta cggctgttga ggtcggtaag catttccacc aggaactggt
     4921 aaccgctgcc atcttcggca tggaacgctg ccgggttgct gcccgcaaac gcaccaatca
     4981 acgaacgaat acggttcggg ttgctcatgg taaatgagcg atgctgcaac agaccacgca
     5041 ccgtctccag cacattcgcc gccgggctgg tggcttgcag gataaaccat ttatccatca
     5101 ccagaccgtc ctgatgccac ttatcgtcgt actcctgcat cagcgcgtca cggcaaggca
     5161 gctgtgcagc aaccgccgca gaaagcgctg ccagcgcatc ggtcatattg tttgcttcat
     5221 ggtattgctt gctcaccagc acgtcagcca gatgcgtttc accaaaagca aggaagcgca
     5281 ggcaggcatt acgcagagtg cgttttgcaa tatcttcatg ctcaacacgg tactcgctct
     5341 ggtaattcgc gttgtaaata gccagcagct catccgccag ttcagtcgcc agagtacgag
     5401 tgagtgcttc gcgtacttcg gcaatagcaa tcgggtcgat gatatcgaac agttcagcca
     5461 tttcattgac agaaggcagc gtcaggattt ctgccgccag cgccggatca atcttctcat
     5521 cgagcagcac cgcgcggaaa gcgtcagcca catgtaccgg cagagacagc ggctgccctt
     5581 gctgatgacg ggcgacgttc agcttgatgt aggttgccag caaactttgc gccgcatccc
     5641 aacgggagaa atcattacgc gcatgacgca tcaggaaggt cagttgctga tcgctccact
     5701 tatattccag tttcactggc gcagagaatt cgcacagcag cgcaggcacc ggctggaagt
     5761 agacattatc aaaaacaaag gtctgttccg cctgggtgac gttcagcacg gaattcaccg
     5821 gatgaccgcc tttctgcaac gggatcactt tgccttcgtt gtcatacaat tcgatggcaa
     5881 atggaatatg cagcggctgt ttttctgcct gatccggcgt ggctggcgtg cgctggctga
     5941 tggtcagggt gtactgctcg gtttccggat tgtagtcgtc tttgacggtc acaatcggcg
     6001 tgccggactg gctgtaccaa cggcggaaat gggagagatc gacattcgac gcatcttcca
     6061 tcgcctgcac aaagtcgtcg caggttgccg cgctgccatc atggcgctcg aaatagagtt
     6121 gcatcccttt ctggaagttt tcttcgccca gcagggtgtg gatcatgcga atcacttccg
     6181 cgcccttctc gtaaacggtc agggtgtaga agttgttcat ctcaatgacc atatccgggc
     6241 ggatcgggtg cgccatcggg ctggcgtctt ccgcaaactg caatccgcgc atggtgcgca
     6301 cattattgat acggtttacc gcgcgggaac caaggtcaga gctgaattcc tgatcgcgga
     6361 agacggttaa accttctttc aggctgagct ggaaccagtc gcggcaggtc actcggttac
     6421 cggtccagtt atggaaatat tcatggccga taacgcgttc aatatcgaga taatctttgt
     6481 cggtggcggt gtcggtgcgg gccaacacat atttggagtt aaagatattc agacccttat
     6541 tctccattgc gcccatattg aagaaatcca ccgcgacgat catatagatg tcgaggtcat
     6601 actccaggcc aaagcgttct tcatcccatt tcatggagtt tttcagcgag gtcatcgccc
     6661 acggcgcgcg atcaaggttg ccgcgatcga cgtacagctc cagtgctact tcgcgaccag
     6721 aacgcgtggt aaaggtgtcg cgcagtacat caaagtcgcc tgccaccagc gcaaacaggt
     6781 agcacggttt cgggaacggg tcctgccact gtacccaatg gcgtccgttt tccagttcac
     6841 cttgcgcaac gcggttaccg ttggaaagca ggaagggata tttggtttta tcggcaataa
     6901 ttttggtggt aaaacgcgcc agcacgtccg ggcggtcgag ataatacgta atatggcgga
     6961 aaccttcggc ttcacactgg gtgcaaagcg catcgcctga ctgataaagc ccttccagcg
     7021 ccgtattcgc cgccgggctt atttcattaa tgatcttaag cgtaaaacgc tccggcaaat
     7081 tactgatgac cagtgcgccc tcttcttctt tccaggcggt ccacggctca tcattaatat
     7141 gaacagaaac cagtttgagg tcttcgccgt tgagacgaag cggagcatct gatgcaccat
     7201 gacggacagc ctggctgacc gcggtaacga ccgtcttttg cgcgtcgagg tcaaaggtca
     7261 agtcaatatc agtaatctgg taatccggcg cacgataatc gtgacggtat ttggcttgtg
     7321 gctgttgagt cataaataac ctttagcatc ttttatagag tctggtgttc agtctattcc
     7381 tgttgcgtaa atcgcgctat gcagaatctt catcttttca ggtacaaacg cctttattgc
     7441 tacattttta taacatacac cgcgtaatgc catcgaccag aaaggtggca tatggtgtga
     7501 tcggggttca ataaattgcg aaacaaggta tactccagca gttcctgaag atgtttattg
     7561 tactaaacgc tcctgtacga ggacgctact gcgcacctat gacacaattc gcttctcctg
     7621 ttctgcactc gttgctggat acagatgctt ataagttgca tatgcagcaa gccgtgtttc
     7681 atcactatta cgatgtgcat gtcgcggcgg agtttcgttg ccgaggtgac gatctgctgg
     7741 gtatttatgc cgatgctatt cgtgaacaga ttcaggcgat gcagcacctg cgcctgcagg
     7801 atgatgaata tcagtggctc tctgccctgc ctttctttaa ggccgactat cttaactggt
     7861 tacgcgagtt ccgctttaac ccggaacaag tcaccgtatc caacgataat ggcaagctgg
     7921 atattcgttt aagcggcccg tggcgtgaag tcatcctctg ggaagttcct ttgctggcgg
     7981 ttatcagtga aatggtacat cgctatcgct caccgcaggc cgacgttgcg caagccctcg
     8041 acacgctgga aagcaaatta gtcgacttct cggcgttaac cgccggcctt gatatgtcgc
     8101 gcttccatct tatggatttt ggcacccgtc gccgtttttc tcgcgaagta caagaaacca
     8161 tcgttaagcg tctgcaacag gaatcctggt tcgtgggcac cagcaactac gatctggcgc
     8221 gtcggctttc cctcacgccg atgggaacac aggcacacga atggttccag gcacatcagc
     8281 aaatcagccc ggatctagcc aacagccagc gagctgcact tgctgcctgg ctggaagaat
     8341 atcccgacca acttggcatt gcattaaccg actgcatcac tatggatgct ttcctgcgtg
     8401 atttcggtgt cgagttcgcc agtcgctatc agggcctgcg tcatgactct ggcgacccgg
     8461 ttgaatgggg tgaaaaagcc attgcacatt atgaaaagct gggaattgat ccacagagta
     8521 aaactctggt tttctctgac aatctggatt tacgcaaagc ggttgagcta taccgccact
     8581 tctcttcccg cgtgcaatta agttttggta ttgggactcg cctgacctgc gatatccccc
     8641 aggtaaaacc cctgaatatt gtgattaagt tggttgagtg taacggtaaa ccggtggcga
     8701 aactttctga cagccctggc aaaactatct gccacgataa agcgtttgtt cgggcgctgc
     8761 gcaaagcgtt cgaccttccg catattaaaa aagccagtta atatcatcag ggagctaatc
     8821 ggctcccttt ttttaccttt aattccgaaa tctttcgctg catttgcgaa ttctgcttgt
     8881 ctgattgcag atgccaggta acataggtat ccccccattt acgggtggac aagtgtttat
     8941 tttttccgac tattaacaga gagaatatta tgagcgttgt gcctgtagcc gacgtactcc
     9001 agggccgcgt agccgttgac agcgaagtca ccgtacgcgg atgggtacgt acccgccgag
     9061 attcaaaagc tggcatctcc ttcctcgccg tttatgacgg ttcctgcttt gatcctgtac
     9121 aggctgtcat caataattct ctgcccaatt acaatgaaga cgtcctgcgt ctgaccaccg
     9181 gctgctcggt cattgtgacg ggtaaagtcg tggcgtcgcc gggccagggg caacaatttg
     9241 aaattcaggc cagcaaggtt gaagttgctg gttgggttga agatccagac acttacccga
     9301 tggcggcaaa acgccacagc attgagtatc tgcgtgaagt cgctcacctg cgtccgcgca
     9361 caaacctgat tggtgccgtc gcgcgcgttc gtcatacgct ggcgcaggcg ctgcatcgct
     9421 tctttaacga gcagggattc ttctgggttt caacgccact gattaccgca tctgataccg
     9481 aaggtgcagg cgaaatgttc cgcgtttcta cgctggatct ggaaaacctg ccgcgtaacg
     9541 atcagggcaa agtggatttc gacaaagact tctttggtaa agagtctttc ctgaccgtat
     9601 ctggccagtt gaacggcgaa acctacgctt gcgcattatc caaaatttat accttcggcc
     9661 cgactttccg tgctgaaaac tccaacacca gccgtcacct ggcggaattc tggatgctgg
     9721 agccggaagt ggcgtttgct aacctgaacg atatcgcggg tctggctgaa gccatgctga
     9781 aatatgtctt caaagcggtt ctcgaagaac gcgcagacga catgaaattc ttcgctgaac
     9841 gcgtagataa agatgccgtt tcacgtctgg aacgcttcat tgaggccgat ttcgcgcagg
     9901 tggattacac cgacgcagtg accattctcg aaaactgcgg caggaagttt gaaaacccgg
     9961 tttactgggg cgtcgatctc tcttctgagc atgagcgtta tctggcggaa gaacacttta
    10021 aagcaccggt agtggttaaa aactatccga aagatattaa agcgttctat atgcgcctca
    10081 acgaagacgg taaaaccgtt gcggctatgg acgttctggc tccgggcatc ggtgagatca
    10141 ttggtggctc ccagcgtgaa gaacgtctgg acgttctgga cgagcgtatg ctggaaatgg
    10201 gcctgaataa agaagattac tggtggtatc gcgatctgcg tcgctacggt actgttccgc
    10261 attcaggttt cggtcttggt tttgaacgcc tgattgctta cgtaactggc gtgcaaaacg
    10321 tgcgtgatgt gattccgttc ccacgtactc cgcgcaacgc cagcttctga ttttcttcct
    10381 caaagagcca gcgcctgctg gctctttttt atcctttctg tcaagttatc tgtttgttaa
    10441 gtcaagcaat ctatttgcaa ccccgccata acgatcatcc tgttacggaa tattacattg
    10501 caacatttac gtacaaaaaa taacccgcat tcttattgcg gattagtttt ttcttagctg
    10561 atagcacaat tttcatacta ttttttggca ttc
//
LOCUS       DAZGLJ010000157        10507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000157 DAZGLJ010000000
VERSION     DAZGLJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.157"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(65..433)
                     /gene="ycgJ"
                     /locus_tag="ACHK49_003520"
     CDS             complement(65..433)
                     /gene="ycgJ"
                     /locus_tag="ACHK49_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO9264104.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDASGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            953..1648
                     /gene="minC"
                     /locus_tag="ACHK49_003521"
     CDS             953..1648
                     /gene="minC"
                     /locus_tag="ACHK49_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO9264105.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1672..2484
                     /gene="minD"
                     /locus_tag="ACHK49_003522"
     CDS             1672..2484
                     /gene="minD"
                     /locus_tag="ACHK49_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO9264106.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2488..2754
                     /gene="minE"
                     /locus_tag="ACHK49_003523"
     CDS             2488..2754
                     /gene="minE"
                     /locus_tag="ACHK49_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO9264107.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(3126..3470)
                     /locus_tag="ACHK49_003524"
                     /pseudo
     CDS             complement(3126..3470)
                     /locus_tag="ACHK49_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3505..3624)
                     /locus_tag="ACHK49_003525"
     CDS             complement(3505..3624)
                     /locus_tag="ACHK49_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO9264108.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3585..3770)
                     /gene="ymgJ"
                     /locus_tag="ACHK49_003526"
     CDS             complement(3585..3770)
                     /gene="ymgJ"
                     /locus_tag="ACHK49_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFO9264109.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3871..4044
                     /gene="ymgI"
                     /locus_tag="ACHK49_003527"
     CDS             3871..4044
                     /gene="ymgI"
                     /locus_tag="ACHK49_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFO9264110.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            4046..4390
                     /gene="ymgG"
                     /locus_tag="ACHK49_003528"
     CDS             4046..4390
                     /gene="ymgG"
                     /locus_tag="ACHK49_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO9264111.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4394..4729
                     /gene="ymgD"
                     /locus_tag="ACHK49_003529"
     CDS             4394..4729
                     /gene="ymgD"
                     /locus_tag="ACHK49_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO9264112.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4786..7107)
                     /locus_tag="ACHK49_003530"
     CDS             complement(4786..7107)
                     /locus_tag="ACHK49_003530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO9264113.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     GSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGAELLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(7224..7433)
                     /locus_tag="ACHK49_003531"
     CDS             complement(7224..7433)
                     /locus_tag="ACHK49_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264114.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            complement(7833..8051)
                     /gene="ymgF"
                     /locus_tag="ACHK49_003532"
     CDS             complement(7833..8051)
                     /gene="ymgF"
                     /locus_tag="ACHK49_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO9264115.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
     gene            complement(8183..9706)
                     /gene="pdeG"
                     /locus_tag="ACHK49_003533"
     CDS             complement(8183..9706)
                     /gene="pdeG"
                     /locus_tag="ACHK49_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO9264116.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETNEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(10038..10286)
                     /gene="ymgC"
                     /locus_tag="ACHK49_003534"
     CDS             complement(10038..10286)
                     /gene="ymgC"
                     /locus_tag="ACHK49_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO9264117.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            complement(10399..>10507)
                     /gene="ariR"
                     /locus_tag="ACHK49_003535"
                     /gene_synonym="ymgB"
     CDS             complement(10399..>10507)
                     /gene="ariR"
                     /locus_tag="ACHK49_003535"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO9264118.1"
                     /translation="ILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
BASE COUNT         2995 a         2417 c         2253 g         2842 t
ORIGIN      
        1 acgccccact cctctctgtc gctcaagagg ttaatgacga atagaacata atgtaaacaa
       61 gacatcactc acgacacata aataacatct ttgtggtggt ttgattgatt tttccactcc
      121 gcttaccatc tacaccaaaa taacgatcat cacgacataa tttttcttta acatcgcaaa
      181 atacaccgtt agcgaatgta aattcgctgg gatcgtagcc ttgtaatgat tgtaagtttt
      241 cagcggcttt ttcgccgaga tatttccttg ttaactctgg ggaaatgcca tctgagttca
      301 cacaaatata cgggtcgcaa acaacgccgc acgccggaga ttgaagtccg ctagcgtcat
      361 gtccagacgc cattgatgag aacatcatcc ccacacctga caatccgata taaataatac
      421 gcatcatatt catagcacat cctgataatc caaccgtaca acacttatgc tcatttcgtc
      481 atcaatccgt aatcaaaacc gctgacctgg acgagttacg atgacctgac agaagtatca
      541 gacgtcttct gcggtttcta tgatagagca attctgtggc ggatattaag ataacgggta
      601 tctgttcttt aatgatgccg caaaaaatgg aaagttaaga aagcctggcg tggtgaaaaa
      661 atacacttgc ctcaatactg gatatttaaa caggtctgga gatgctgtca cgattagggt
      721 gcggaaaaag gaagcgtaaa taagaagaat caaaagtacg acggcaatgg gttgattgac
      781 aagggtattt tttaagctat gaatcagcgc catttatcac agaatagact tttactctga
      841 ataaatggga gggtgacttg cctcaatata atccagacta taacatgcct tatagtcttc
      901 ggaacatcat cgcgcgctgg cgatgattaa tagctaattg agtaaggcca ggatgtcaaa
      961 cacgccaatc gagcttaaag gcagtagctt cactttatct gtggttcatc tgcatgaggc
     1021 agaacctaag gttatccatc aggcgctgga agacaaaatc gctcaggccc ccgcattttt
     1081 aaaacatgcc cccgttgtac tcaacgtcag tgcactggaa gacccggtaa actggtcagc
     1141 gatgcataag gcggtttcgg caaccggttt gcgggttatt ggcgtaagtg gctgcaaaga
     1201 tgcgcaactt aaagccgaaa ttgaaaaaat ggggctgcct atcctgacgg aaggaaagga
     1261 aaaagcgcca cgtccagctc ccgcaccgca ggctccagcg caaaatacaa cgccggtcac
     1321 aaaaacgcgt ttaatagata ccccggtgcg ttccggtcag cgtatttatg ctccacaatg
     1381 tgatctgatt gttacaagcc acgttagcgc tggggccgaa ttgattgccg atggtaacat
     1441 tcatgtctat ggcatgatgc gcggtcgtgc gctggcaggg gcaagtggtg accgggaaac
     1501 gcaaatattt tgtacgaacc tgatggcgga actggtgtcc atcgcaggtg aatactggct
     1561 gagtgatcaa atcccagcag aattttatgg caaagcggcg cgactgcagt tagtcgaaaa
     1621 cgctttgacc gttcaaccgt taaattgatc cctttttaac aaggaatttc tatggcacgc
     1681 attattgttg ttacttcggg caaagggggt gttggtaaga caacctccag cgcggccatc
     1741 gccactggtt tggcccagaa gggaaagaaa actgtcgtga tagattttga tatcggcctg
     1801 cgtaatctcg acctgattat gggttgtgaa cgccgggtcg tttacgattt cgtcaacgtc
     1861 attcagggcg atgcaacgct aaatcaggcg ttaattaaag ataagcgtac tgaaaatctc
     1921 tatattcttc cggcatcgca aacacgcgat aaagatgccc tcacccgtga aggggtcgcc
     1981 aaagttcttg atgatctgaa agcgatggat tttgaattta tcgtttgtga ctccccggca
     2041 gggattgaaa ccggtgcgtt aatggcactc tattttgcag acgaagccat tattaccacc
     2101 aacccggaag tctcctcagt acgcgactct gaccgtattt taggcattct ggcgtcgaaa
     2161 tcacgccgcg cagaaaatgg cgaagagcct attaaagagc acctgctgtt aacgcgctat
     2221 aacccaggcc gcgtaagcag aggtgacatg ctgagcatgg aagatgtgct ggagatcctg
     2281 cgcatcaaac tcgtcggcgt gatcccagag gatcaatcag tattgcgcgc ctctaaccag
     2341 ggtgaaccgg tcattctcga cattaacgcc gatgcgggta aagcctacgc agataccgta
     2401 gaacgtctgt tgggagaaga acgtcctttc cgcttcattg aagaagagaa gaaaggcttc
     2461 ctcaaacgct tgttcggagg ataagttatg gcattactcg atttctttct ctcgcggaag
     2521 aaaaacacag ccaacattgc aaaagaacgg ctgcagatta ttgttgctga acgccgtcgc
     2581 agcgatgcag aaccgcatta tctgccgcag ttgcgtaaag atattcttga ggtcatttgt
     2641 aaatacgtac aaattgatcc tgagatggta accgtacagc ttgagcaaaa agatggcgat
     2701 atttctattc ttgagctgaa cgtgacctta ccggaagcag aagagctgaa ataagcccgc
     2761 tgtaaaaacg catttatctt caaggcagag tcatttctgc cttgagtttt tcatccctct
     2821 catccacgtt gtggtaaagc ggcgagtatt cttgctgata ctcctcattg ctatttccac
     2881 ctcccccttt acaccttaag gctgttccca cgcacagaac aaaatgttta tgcctggttg
     2941 agtaaataac ctcattgtta gtgtggatac actgggggat gataaccccg caatttcatt
     3001 attcattgtt ctcccatagg atgaggtttc ccgccgtaac tggcgggctt tttttgcccc
     3061 agaattttcc cttttagccc cctgtaaacg aaaactgcgc cgaagcgcag tttaatcgtt
     3121 gatggtcaga acgaccagtt cacaccagcc accgcattcc acggggattc cacaccggca
     3181 ccatggctat accccacccc gaaatgcccg cttaacgtac tgctgaatga ggctttaata
     3241 cctgcctggt atattccacg tctgcccgac aaatcattga caaaattacc gtcactattc
     3301 actttcaccc gattatcatc gacaaattct ttgctcacag ccgccttcag gcagggttca
     3361 atttccatac cgttccccag acgcatgttg taactcagcg ttgcacccag ttcacgatat
     3421 atactgcggg tatcgactga tttcgattcc atgccattgg ataaatgata gtagcgtcta
     3481 agtgacctaa gctctgctgc aacatcagcc cgcaggcacc agaccagggg attcatcctg
     3541 aagagacagc gcaggtgtat tgtgttcacc gctcaccaaa gacatcatga tgaaatgatg
     3601 atatcccgca taagaaagag gcatttttta cacgcagtgc gctgaagtgt agttggataa
     3661 aaaatcactc ttttcagata atactgtcgt acgcttatct ttcgacaatg aggccgtcgg
     3721 caaaataaaa tgactaatgt aatcgttgct gatgaatatc ttctgctcac ataaaaatca
     3781 cacaataata ttgataaatc agattatctt acctttacaa cattgcgtta acagtcaatg
     3841 acacttcttg aataaaaata ttgtcaatca atgagttatt cctcatttaa gattattctc
     3901 attcacgtaa aaaacataat tcctatcatc acagccacgc tcattttgaa ttatctgaat
     3961 aatagcgagc gttctttagt taagcagatc ttaattgaag acgaaattat tgtgtgtgca
     4021 acttacttaa taccggacat ctaagttgaa gaaaaaaata cttgccttcg ggcttatctc
     4081 agcattgttc tgctccacgc cagcgatggc ggatatgaat cgcaccacga aaggtgcatt
     4141 gctaggcgca ggcgttggtt tacttactgg caatggtgtt aatggcgtac tgaaaggtgc
     4201 agctgttggc gctggtgttg gtgcagtaac agaaaaaggc cgcgacggta aaaatgcgcg
     4261 taaaggtgca aaagtaggtg ccgccgttgg tgctgtcact ggcgttctga ccggcaatgg
     4321 cctcgaaggg gcgattaaag gtgccgtgat tggcggtacg ggtggtgcaa ttctgggtaa
     4381 aatgaaataa ggaatgatga tgaaaaaatt tgctttactg gcgggcctgt ttgttttcgc
     4441 accaatgacc tgggcgcagg actacaatat taaaaacggt ttgccgtcag aaacctatat
     4501 tacttgcgca gaagccaacg aaatggcaaa aactgacagc gcacaggttg cagaaattgt
     4561 tgcggtaatg ggtaatgcca gcgttgccag ccgtgattta aaaattgagc aatctccgga
     4621 actgagcgcc aaagtggtag aaaaactgaa tcaggtttgc gctaaagacc cgcaaatgct
     4681 gttgatcacg gcaattgatg acaccatgcg tgctatcggc aaaaaataat atttcatatg
     4741 taccgcgtcg atgaatacga cgcggttttt tagctaacgg cgggatcaga acttatattt
     4801 caggcccacc atcactgcgg tgtcattgta gccattatca cccagctgca cgcccacatt
     4861 cccccagacg ctggccgctg gattcaggtt gccatttacg ccaagtttaa tttcccccaa
     4921 attactcgcc ccatcttgtt cgactttcac accgttcatt gagacagcaa aatctttact
     4981 gttatggatc cagttcattt caacaaaagg ttcaaagtcg tcaccgtgcg gaccgctgtt
     5041 tttctcctga gtacgaataa aggtgcggaa accaagtcgg gtctggatgt tgttatttgc
     5101 gtcattctca atacgtgagc cattggcttc ggtgtgcgca ttatgatcga cgcccatcca
     5161 gatagcctgt gcctgtggct ggaatatcca cgtgtatttc gccgcttcca gaccaaagga
     5221 tttattcagg gcaaagccgt aacccgcttc cagcgagacg gttgcgcctt tcgcactata
     5281 ggattcatag gctaattcat cccctttcac cgagttttta aaccagctat attgcgccca
     5341 ggcgtcaatg tatgcgcctt ttttactgat gtcatcggca aaccaagtgg catacagccc
     5401 tgcgcaatag cctctgacac tccctttcga acgataatcc gacacgctgg aatgagttga
     5461 gttgtagtcg cgggcataac cagccatcac tcccaaacgc caactgtcac tgtcggtaaa
     5521 accaccggtt aacagctcgg cccctaactg cgagacgtag cgatgcgagg tcgttctcaa
     5581 ctgtccgttg ctgtcacgcc aggcattatg cccgccaatt tgacgtagcc aaagtcggct
     5641 tgaacgctcc tgttcagtta caggatcgat gtagcgcatt tcacccgcac ggtcgttcag
     5701 acgcataaca aaaagagtgt tagcggctgc caggttggcg acgtaacttc cagcttcgag
     5761 gcgcaggacg ggggtaggcg caacagtagg cgctgaactt cctccggagt ttgtttcacc
     5821 gccgctcgtt gcctggaagt tagtcagata ccagttttta ttggtcgctt cggtattacc
     5881 tcgggttaat gaatattcgt aggcaccggc gaaaatcctc gaatccttaa taaattcccc
     5941 atttgattcc ccctcaacgc tgataatctc tatcccattt aacgtgtaag ctccgctgcc
     6001 tcctgcattg gtaactttaa ctcgggttgt tccagaggta tttccattaa taacgacttt
     6061 atccgtagct gaattatcat cacccaattc agttctgaga tgcaatacgc cattattacc
     6121 aacgtaattt tcagttattg ttaatcttgt tggttcgaca tcccgtccat ctgcccggga
     6181 aatatagacc gtactattat caaccgtgag gttgctcacc gttgagtcag ctttaatatc
     6241 ccaggtacta ttatctgaaa gcgacagata ggtatgagtg ttaacatctg ttgaaatatt
     6301 agctgaaccc gttatttttg aattagttgc atcgacggtc aaatgacctt taatattatt
     6361 gataaaaaca tctgcagccg atgatacctc actatccgta atggtcacgt tactcgtctc
     6421 atttttatgg tagaggattt catttgctga ccctagtgag ctaccagata aaatatctac
     6481 cgttgcttcc tgaccattta tggaagactc cagcacaatt ccagacccct gatgcgcgac
     6541 tgcagatgta tttttcagcg tcaccgtgcc gccattataa attttagcag tcgcggcgtc
     6601 aggacccgtt gtataaattt ctccctggtt aaatgaaact gtcccctggc gggcagcgat
     6661 ggcatgagca ttgtccccat gcgtggtgat atcaacatcg gttaatgtaa cgctggcatt
     6721 ggtataatca agcgtgtcac cagccttgac gacagtgcca ttagcgtacg cggcataact
     6781 ttctgttccg tgggttgtga tctccccttt tgccatatcc agttttgcta tggcattatt
     6841 ttcattactt cctgtatcgg tagtactttc ttcatcaaga gaagatacac acgcacgcgc
     6901 gcttccggga gatgcacacg caagaacgcc gactgcatga ttacccgcag taacaataaa
     6961 tgtatcatct ccgtcagaaa aagagggggt aaccgaaccg tcagagttga ctcttattat
     7021 ggtgtcagta ttcaggtttc cactcgtttg acttagcaaa ccaattgagt aatcccccgt
     7081 tatgacaatc gtgtttttac cctgcaaaat aacagcaccg ccaaattcac ttacgattgc
     7141 gctagctgtt aatgctgtgc ttagaggact attgtcagaa ggatataaac cgcccgttgt
     7201 tacaatgacg ccattgttaa tggttaactt gctaccctct cctgcaacgg atacccgtaa
     7261 cttccattac ccgttgttat aactagcgca ttatcaatat cttttacttt tccatctttt
     7321 attgccacag catgtttata ttcatcgtgc tgaataaact gttcagcata tgcattaccc
     7381 accgccagag caataagtcc caaactaaac ccggagagtt ttttcagttt catagattaa
     7441 ccatccattc attggattaa caaacattcc tggtcaaata acctatgcgt tcagattacc
     7501 aaaacggagg gtaaagacaa aaacaaaagc acaaaaaatt taaatagata aacagcgaca
     7561 atatataaat gataaataag tcggcattat catttttcac cttattttca tgacattgat
     7621 cactttgagg tgacgatttt gtttaacaaa taactcttcg acatcacaat cattgatagt
     7681 tgcaataaaa attatttcaa tgacatataa aaacaactgt gataacacca gggttgacaa
     7741 ctatttgcgc aataattcct ttacttcaat gatccaggca ttcactgcca gcaacccatt
     7801 cagccgcata ctgacagtga gaaactgaaa gattattgct gatccctgtg gctgacgagt
     7861 ctttccctta atcgattaac cagattgatc gccagccaaa caagggcatt aagcaaattt
     7921 atcgtcacaa gcacttctcc cagcccggta ctgggttttg caataaggat gaccaggagg
     7981 gtgatcagca taaatgcaat ggaaaagatg atatagagag tgaaataatc cagattatta
     8041 ctattgttca tgctactaat ggcaatcctg tcaaaacttt caaacaatta tagcaaacaa
     8101 aataatgaac aatagtaaca agaggcaacg agctaattag ctgtctataa ttacaccgca
     8161 ctatggcctt actgatcaat tttcactcaa ccacaacctt caccttcggt ttagaaagga
     8221 taattttgac caggtcgatg tatgtaacag gtttatagaa ataataaccc tgctgaaatg
     8281 tgatattatt ttggttcaga tagtcaagtt gttcattcgt ttcgacacct tcagcgacga
     8341 tactgatcga aagtttacgc gccagatcca atacacaatc cagaattcgg gttgattctg
     8401 ggtctgcgtt tacgcggcca acgaaactgt gatcaatttt gatataatca atatgcaggt
     8461 catgaagata agaaagccct gagtagccag taccgaaatc atccagcgcg atgacaaaac
     8521 cattttcatg cagaatgttc aaccgctgaa ccagactttc atcaacattc aatggctcac
     8581 gctcggtgac ttccagaaca aggtttaaat cgcggcgggt aaaactgtca cggaaattta
     8641 aacactcgtc gacaaacgtc ggcgaaataa tatgcgaagc gctaaaatta attccaatat
     8701 gaaaaccttc cggcaattta ctcgcgatag cgttcatctg tctggcaacc tgattcatca
     8761 ggctttgcgt aagcggaacg attaatccgg atttttcagc aagtggaata aatgccgcgg
     8821 gtgatatata tccaccgtga ggttgtttcc agcgggctaa cacctcaact ccccgcaatg
     8881 tcccttcccg accatttacc acaggttggt aaaaagggac gatctcccca ttatttattg
     8941 cccgtcgcag gatctcttca ggcgttgcgc ttttattgaa atatctatca agcaggaagg
     9001 cagcggcgca ggcgattaac agaataaata ttaaaatccc aaagccttta tcgataagtc
     9061 tgtagaagct aaagagtggc ggcgggttaa agataatagt gaaatgatat tttgtggagt
     9121 gaaccttctc ggtataggga taatgtccat taagcgtcat cacatcaccc gttggtccaa
     9181 taatgtcatc cgccacgcgt aatacatacc ttaccccttt caagggtaca ttaagtgccc
     9241 cacgaatatg ctgatcgctt atcgtaacca aaatgcgcgt atctgcaaat tggttctgat
     9301 agagcaggat aggtaatcta ttaacggtgt cgattgctgg agccaacagt aaattactgt
     9361 ccgggaaaac aggaatacga gacaacagga cccgattccc aggcagggat gtacaccaga
     9421 ctattccctg tttgagaatg atgattgtgc gcaggtgagg tttcagagct gcttcagtgc
     9481 caagctgata ttgctcctcg aggttgcatt ccttcccggc aatgttaacc gctgtttgag
     9541 tcgcttgtga cgcttcttca agtatatgat tgatattgtt ggcggcatat ctcgctcccg
     9601 ccaggtactc tgcttttgcg gaataccaga gctggatgtt caaaatagcg acaccggtaa
     9661 taaacaagca tatcgcaacg aggatgggta tgagtgtatt gcgcatttgc ttgtcccggc
     9721 caaagggttt aatacatgaa aatgccattc attcgctcgc gctggcatct acagagagca
     9781 tggtagagag cataagaaat atccacgccg aagataaaat tttctccatc ttgatctaac
     9841 ccattaatta ttgagcataa tgtaggcagt acaaaataag ttaggcggga tatcaggcgt
     9901 caagaatgga gagcgagaac tctccattct tgacacctga tattgcggac ataataagaa
     9961 agcataacgc ctgaaatgct cactttgcat cagcatggtg atacagctga tgtttattct
    10021 aaaaccttac tcaagttcta agagagcacg gattccctgt cattaataat gatatccgtt
    10081 agtgtagtct tatgcatttc aatgtactgc aatatttgca gatagagatc agaaagaatc
    10141 acactggttt catcttttaa acgcaagcgt aaagcctgat caataatatg actatcaaca
    10201 tacccatgcg tcatgaacac ctccctctcg agatttttaa acaaagcaca ttgaaaaata
    10261 aatctctctg ggattgaatt attcatgtca gcctcgtaga aatattaacc caataaaatt
    10321 atgctgtgta tagtctaaag cgacagacaa aaataagatg aatgagtttt ctataaactt
    10381 atacttaata attagaagtt acatatcatc agctgtgtat cgcaacacga tttccagtgt
    10441 ttttctaatc acatcagctt tgagaatatc acttgttgtt tccagggagt gtatcagact
    10501 taagata
//
LOCUS       DAZGLJ010000158        10480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000158 DAZGLJ010000000
VERSION     DAZGLJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.158"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..2066
                     /gene="speF"
                     /locus_tag="ACHK49_003536"
     CDS             <1..2066
                     /gene="speF"
                     /locus_tag="ACHK49_003536"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO9264119.1"
                     /translation="GKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRK
                     EFYGRQLETAASHYETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFV
                     EYFGEMLFRSDLCNADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKV
                     VLNALLTPGDLVLFDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEES
                     YLRELIAEVAPQRAKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWV
                     GYEQFIPMMADCSPLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRY
                     VPHKRMNNAFMMHASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILD
                     NCQHIRPFVPELVDGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCK
                     LLLTTPGIDARNGEYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQ
                     QLVALLVRFEKLLESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQL
                     QKEMFRKEHFPRVSMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVP
                     GEIWGGAVLRYFSALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLL
                     KGEKL"
     gene            2063..3382
                     /gene="potE"
                     /locus_tag="ACHK49_003537"
     CDS             2063..3382
                     /gene="potE"
                     /locus_tag="ACHK49_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO9264120.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3438..5078)
                     /gene="pgm"
                     /locus_tag="ACHK49_003538"
     CDS             complement(3438..5078)
                     /gene="pgm"
                     /locus_tag="ACHK49_003538"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO9264121.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGT
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNALGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5104..5649)
                     /gene="seqA"
                     /locus_tag="ACHK49_003539"
     CDS             complement(5104..5649)
                     /gene="seqA"
                     /locus_tag="ACHK49_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO9264122.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSASSQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVRGTI"
     gene            5834..6598
                     /gene="ybfF"
                     /locus_tag="ACHK49_003540"
     CDS             5834..6598
                     /gene="ybfF"
                     /locus_tag="ACHK49_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO9264123.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6738..7031
                     /gene="ybfE"
                     /locus_tag="ACHK49_003541"
     CDS             6738..7031
                     /gene="ybfE"
                     /locus_tag="ACHK49_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO9264124.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7171..7701
                     /gene="fldA"
                     /locus_tag="ACHK49_003542"
     CDS             7171..7701
                     /gene="fldA"
                     /locus_tag="ACHK49_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO9264125.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7911..7997
                     /gene="uof"
                     /locus_tag="ACHK49_003543"
     CDS             7911..7997
                     /gene="uof"
                     /locus_tag="ACHK49_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO9264126.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7990..8436
                     /gene="fur"
                     /locus_tag="ACHK49_003544"
     CDS             7990..8436
                     /gene="fur"
                     /locus_tag="ACHK49_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO9264127.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8520..8846)
                     /gene="chiQ"
                     /locus_tag="ACHK49_003545"
     CDS             complement(8520..8846)
                     /gene="chiQ"
                     /locus_tag="ACHK49_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO9264128.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8896..10302)
                     /gene="chiP"
                     /locus_tag="ACHK49_003546"
     CDS             complement(8896..10302)
                     /gene="chiP"
                     /locus_tag="ACHK49_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO9264129.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2507 a         2621 c         2657 g         2695 t
ORIGIN      
        1 gtggaaaact cgatgaaata gacgctactg gctatgacat tcctgttttt attgcaacgg
       61 aaaatgaaga acgtgtaccc gcagaatatc tgccacgtat ttctggtgtc ttcgaacatt
      121 gcgaatcacg taaagagttt tatggacgtc agttagaaac cgctgccagc cattatgaaa
      181 cgcaactgcg cccgcccttc ttccgcgcac tggtcgatta tgttaatcag ggcaatagtg
      241 cttttgactg ccctggtcat caggggggtg agtttttccg tcgccatccg gcaggcaatc
      301 agttcgtgga atactttggc gaaatgctct tccgctctga cttgtgtaat gctgacgtag
      361 caatgggcga tctgctgatt catgaaggtg cgccatgtat tgcacaacag catgcggcta
      421 aagtctttaa tgccgataaa acctatttcg ttttaaacgg tacttcatct tctaataaag
      481 tggtcttaaa cgcattgctg acgccgggcg atctggtgtt gtttgaccgc aataaccata
      541 aatctaacca ccacggtgcg ttgctacagg ctggcgcgac accggtttat ctggaaaccg
      601 cacgtaaccc gtatggcttt atcggtggca ttgatgcgca ctgttttgaa gaaagttacc
      661 tgcgtgagct gatcgcggaa gtcgcaccgc agcgggcaaa agaggctcgt cctttccgcc
      721 tcgctgtgat tcagttaggc acctacgacg gtacgattta taacgcccgc caggtggtgg
      781 ataaaattgg tcatctgtgt gactacattc tgtttgactc cgcatgggtt ggctatgagc
      841 aatttatccc gatgatggct gactgttcgc cgctgttgct ggatcttaat gagaacgatc
      901 cgggtattct ggttacgcaa tccgtacata aacagcaggc tggtttttct caaacttcac
      961 aaatccataa aaaagacagc cacatcaaag ggcaacagcg ttatgtaccg cacaaacgca
     1021 tgaacaacgc ctttatgatg catgcctcca ccagcccgtt ctatccgctg tttgccgcac
     1081 tggatatcaa cgccaaaatg catgaaggtg tcagcggtcg taatatgtgg atggattgtg
     1141 tggtaaatgg cattaatgcc cgcaagctga tcctcgataa ctgtcagcat attcgtccgt
     1201 tcgtacctga actggtggat ggtaaaccct ggcagtcgta tgaaacagcg caaattgcgg
     1261 ttgatctgcg cttcttccag tttgtaccag gtgagcactg gcactctttt gaaggctatg
     1321 cagagaatca atactttgtc gatccatgca aactgttgct gacaacccca ggtattgatg
     1381 cacgtaacgg cgaatatgaa gcgttcggtg tacccgcgac gattcttgct aacttcctgc
     1441 gcgaaaatgg cgtagtgccg gaaaaatgcg atcttaactc catcctcttc ctgctgactc
     1501 cggcagaaga tatggccaag cttcagcaac ttgttgccct gctggtacgc ttcgaaaaac
     1561 tgcttgagtc cgacgcgcca ttagcagaag tgctgccttc catctacaaa cagcatgaag
     1621 agcgctacgc cggttatacc ctgcgtcagt tgtgtcaaga aatgcatgat ttgtatgccc
     1681 gtcacaacgt gaaacaactg caaaaagaga tgttccgtaa ggaacacttc ccacgcgtaa
     1741 gcatgaatcc gcaagaagcc aactacgcct atttacgcgg tgaagtggag ctggttcgtc
     1801 tgccggatgc agaaggccgt atcgctgccg aaggtgcgct tccttatcct ccgggtgtgc
     1861 tgtgtgttgt tccgggtgaa atctggggtg gtgctgtttt gcgttacttc agcgctctgg
     1921 aagaagggat caacctgctg ccaggttttg caccggagct gcagggtgtc tatatcgaag
     1981 aacatgatgg tcgtaagcaa gtttggtgct atgtcatcaa gcctcgtgat gcgcaaagca
     2041 ccctgttgaa aggggaaaaa ttatgagtca ggctaaatcg aacaaaatgg gcgtcgttca
     2101 gttaaccata ctgacgatgg tcaacatgat gggctccggt atcatcatgc tgccgacaaa
     2161 gcttgccgaa gttgggacaa tttcaattat ctcctggctg gtgacagccg taggctcaat
     2221 ggcactggca tgggcattcg caaaatgcgg tatgttcagc cgtaaatcag gcggtatggg
     2281 cggttatgcg gagtatgctt tcggtaaatc cggtaacttt atggcgaact atacctacgg
     2341 cgtctcgctg ctgattgcta acgtcgcgat tgctatttcg gcggttggtt acggcaccga
     2401 actgctcggc gcgagtttgt cgccagtgca gattggtctt gcgaccatcg gggtgctgtg
     2461 gatttgtacc gtggctaact ttggtggtgc gcgcattacc gggcaaatca gtagcattac
     2521 cgtgtggggg gtcattattc cggtggtagg cctgtgcatt atcggctggt tctggtttag
     2581 cccgacgctt tacgttgatt cctggaatcc gcatcatgca ccgttcttca gtgcagtagg
     2641 ttcttccatc gctatgacgc tgtgggcttt tctcggtctg gagtctgcgt gtgcgaatac
     2701 tgatgtagtg gaaaacccgg aacgtaatgt gccaatcgcg gtactcggcg gtacgttagg
     2761 tgcggcggtg atttatatcg tctccaccaa cgtgattgca gggattgtgc ctaatatgga
     2821 gctggcaaat tcaacggcac catttggcct ggccttcgca cagatgttca caccagaagt
     2881 gggtaaagtc attatggcgc tgatggtgat gtcctgctgc ggttcgctgc ttggctggca
     2941 gttcaccatt gcccaggtgt ttaaatcttc atctgatgaa ggctacttcc ctaaaatttt
     3001 ctcccgtgta accaaagtgg atgcaccggt gcagggaatg ttgaccattg tgattatcca
     3061 gagcgggttg gcgctgatga cgattagccc gtcgctaaac agtcagttca acgtgctggt
     3121 taacctggcc gtggtgacca atatcattcc gtatattctg tcaatggcgg cattagtcat
     3181 tattcagaag gtcgctaatg tgcccccctc aaaagcgaaa gttgcaaact ttgttgcttt
     3241 tgttggcgcg atgtatagct tttatgcgct gtactcatcc ggggaagaag ccatgctgta
     3301 cggttccatc gtgaccttcc ttggttggac actgtatggt ctggtatcac cacgctttga
     3361 actgaaaaat aaacacggtt aaaaaagcaa agtaaaaaag ggcgatcttg cgaccgccct
     3421 ttttttatta aatgtgttta cgcgtttttc agaacttcgc taacaatctc aaccgcttct
     3481 ttctcaatct gcttgcgatg ttcttcaccg aggaagcttt cgcagtagat cttatatgcg
     3541 tcttccgtgc ctgacggacg cgcggcgaac cagccgttgt cagtcatcac tttcagaccg
     3601 ccgatagaag caccattacc cggtgccgcc gtcagacgtg cggtgatcgg gtcacctgcc
     3661 agggtgctgg cgctcaccat ttccggagac agcttagaca acgccgcttt ttgtgcggaa
     3721 gtcgcagcgg cctgcaaacg gttgtagctc ggcgcaccaa agcgttttgc cagttcgttg
     3781 tagtgttcct gcgggttctt accggtgaca gcggtgattt ccgccgccag cagacacata
     3841 atgatgccgt ctttatcggt ggaccacggc gtgccgtcga aacgcaggaa ggaagccccg
     3901 gcgctctctt cgccgccaaa gccgaaactg ccgtcgaaca gaccatcaac aaaccatttg
     3961 aatcctaccg ggacttctac cagtttacgg cccaaggcgt tgaccacacg gtcgatcatc
     4021 gcagaagaaa ccagcgtttt accgacagca acatctttgc cccactgcgg acgatgctgg
     4081 aacaggtagt tgatcgccac cgccaggtag tggttcggat tcatcaaacc tgccggagtg
     4141 acgataccgt gacggtcata atccgggtcg ttagcaaacg ccagatcgaa cttatcacgc
     4201 agtgccagca ggcccgccat cgcacactcg gaggagcagt ccatacggat cgtgccgtct
     4261 ttatcaaggt gcataaagcg gaaggtttga tcgacctgat cgttaacgat ggtcaggttg
     4321 aggttgtaat actcgccaat acgcttccag tattcgatac cggaaccgcc cagcggatca
     4381 acgccaagcg tcaggcccgc tttctgaatc gccgccatat caacgatatc ggccagacct
     4441 tccacaaacg gctgcaccag atcctgctct ttcacatgac cggatgccat cgcttcgtcg
     4501 agagagatac gcttcacgcc tttcaggcca tcggccatca gtgcgttggc cctgtcttcc
     4561 accactttgg tgacgttggt atcagccggg ccaccatttg gcgggttgta cttgatacca
     4621 ccatcttccg gcgggttatg ggacggtgta atcacgatac cgtctgccag cgggccacct
     4681 tttttattgt gaaccaggat ggcattggaa acggcaggcg tcggagtgaa tccattgttt
     4741 tcctgcacaa tgacatcaac gccgttcgct gccagcactt ccagtacgga aatgaacgca
     4801 ggctcggaca gggcgtgagt atctttaccc acatagcaag ggccagtgat gccatttttc
     4861 gcacgttctt cagcaattgc ctgagcgatc gccagaatgt gcggctcgtt aaagctgtgg
     4921 cgcgctgcac tgccacggtg accggaagta ccgaatttca ccgcgtgctc cgcattccct
     4981 gcttctggtt tcagtacata atattgcgcc gtcagttggg cgacgttaat caaatcactc
     5041 tgttgtgcag gttggcctgc acgattgtgg attgccattg ctttgtcctt tgtctgcaac
     5101 gttttagata gttccgcgaa ccttctcaat caattccgcc gggaattgca tcgactgcat
     5161 gatgtgttcg atcatgctgc atttacggcc ggtgttggtg ttggtgatca cccaatacgg
     5221 cgtgcctggc acatgtttcg gcttggtctg attaccattt ttcagcagcg tttgttcatc
     5281 tgccgcaaag taaacgcgtg tacgaccgtg caacgattcc gttgcttcgg caaacgcctg
     5341 ggcgtcaaga gaatatagtg tagacaacag cagcataaag cgattgaccg ctcgcttttg
     5401 ctctgcgtat tcgtccgaaa gcagaagttc acgcattgcg cgaaccttgt ctttaatcgt
     5461 tttgaccggc ttcgcttcga cgatagcagg tgacgcaacg cgaacctctt tcgtcaccgg
     5521 agcagcaggc tgtgatgagg cggaaaattt caacatacgc cgtaaaatgt cggatgcgct
     5581 ctcgccgata tgcttagtgt ggctggcaat atagctgtag agttcatcat caacttcaat
     5641 cgttttcatc ttaatccagt gcagtgtctt agctgaatac aagtcgccag gagtatatga
     5701 ataaatcccg gcagcggata gcgtcaagcc tggcttgcag aaaaactcgg aataaacctt
     5761 atttgttgca gccctgtggc acaatggtca acataatacc ctaacccgac aacgcgaaaa
     5821 aaagaacttt gccatgaaat tgaatatccg cgcgcaaact gcacaaaacc agcacaataa
     5881 ttctcccatc gttcttgtcc atggtctgtt tggtagcctc gacaaccttg gcgtactggc
     5941 tcgcgatctg gtaaacgatc acaatatcat ccaggttgat atgcgtaacc acggtctttc
     6001 accgagagat ccggtaatga attacccggc gatggcgcag gatcttgttg atactctgga
     6061 tgcacagcag atcgacaaag caacatttat cggtcactcc atgggcggta aagcggtaat
     6121 ggcacttact gcactggccc ccgatcgcat cgataaactg gtggcgatcg atatcgcacc
     6181 ggtcgactat cacgtacgcc gtcatgatga gattttcgcg gctatcaacg cggtcagtga
     6241 atcggacgca caaactcgcc agcaagcagc agcaataatg cgccagcatc ttaatgaaga
     6301 aggggtgatt cagtttctgt tgaaatcttt tgttgacggg gagtggcgct ttaacgtgcc
     6361 ggtattgtgg gatcagtatc cgcatattgt gggttgggag aaaatcccgg catgggatca
     6421 ccctgccctg tttatccctg gcggcaattc tccgtatgtt agcgagcagt accgtgatga
     6481 tttactggca caatttccac aggcacgggc gcatgtgatt gcaggcgcgg gtcactgggt
     6541 ccatgctgaa aaaccggatg cggtattacg cgctatccgt cgctatctca atgattaacg
     6601 caactgatta aaaacccagc gtccgcgtcg ctttccgcgc gtgggcgttg gcgtgactca
     6661 tcgcgctgat gtattatggc gcgctatcca tccgggcaga ggcctggctg attgtttccc
     6721 ccgaagtcac caagatcatg gccaaagaac aaacggaccg tacgacatta gatctgttcg
     6781 cgcacgagcg tcgaccggga cgaccgaaaa ctaatccgct ttcgcgcgat gaacagctgc
     6841 gtattaataa acgcaaccag ctaaaacgcg acaaagtacg tggccttaag cgtgtcgaac
     6901 tgaagctgaa cgcggaagct gtcgaggcgc tgaacgagct ggcggagtcg cgcaatatga
     6961 gccgtagcga actgatcgaa gagatgttaa tgcagcaact ggcggcgctg cgtagtcagg
     7021 gcatcgttta aatttccact ttcatgtagc acagtgtgca gtcctgctcg tttgctgatt
     7081 ccgcaagact gcctgttctg ctatgattgc ctttatccgt gggcaatttt ccacccccat
     7141 ttcaataagt ttcaagaggt tatttcactc atggctatca ctggcatctt tttcggcagc
     7201 gacaccggta ataccgaaaa tatcgcaaaa atgattcaaa aacagcttgg taaagacgtt
     7261 gccgatgtcc atgacattgc aaaaagcagc aaagaagatc tggaagctta tgacattctg
     7321 ctgctgggca tcccaacctg gtattacggc gaagcgcagt gtgactggga tgacttcttc
     7381 ccgactctcg aagagattga tttcaacggc aaactggttg cgctgtttgg ttgtggtgac
     7441 caggaagatt acgccgaata tttctgcgac gcattgggca ccatccgcga catcattgaa
     7501 ccgcgcggtg caaccatcgt tggtcactgg ccaactgcgg gctatcattt cgaagcatca
     7561 aaaggtctgg cagatgacga ccactttgtc ggtctggcta tcgacgaaga ccgtcagccg
     7621 gaactgaccg ctgaacgtgt agaaaaatgg gttaaacaga tttctgaaga gttgcatctc
     7681 gacgaaattc ttaatgcctg atgtgatgcg gcgtagactc atgtctacgc cgtattaata
     7741 gataatgcca atcaaaataa ttgctacaaa tttgtaactt ttgctgttgt acctgtacaa
     7801 tgtcccggtg ttcaagtggc cttgccgttg taaatgtaag ctgtgccacg tttttattaa
     7861 caatatttgc cagggacttg tggttttcat ttaggcgtgg caattctata atgatacgca
     7921 ttatctcaag agcaaattct gtcacttctt ctaatgaagt gaaccgctta gtaacaggac
     7981 agattccgca tgactgataa caataccgcc ctaaagaaag ctggcctgaa agtaacgctt
     8041 ccacgtttaa aaatcctgga agttcttcag gagccggaca accatcacgt cagtgcggaa
     8101 gatttataca aacgtctgat cgatatgggt gaagaaattg gtctggctac ggtatatcgc
     8161 gtactgaacc agtttgacga cgctggtatc gtcacccgcc acaattttga aggcggtaaa
     8221 tccgtatttg aactgacaca gcaacatcac cacgatcacc tgatttgcct cgactgcggc
     8281 aaggttatcg aatttagtga tgattccatc gaagcgcgtc agcgtgaaat tgccgcaaaa
     8341 catggcattc gcctgactaa ccacagtctc tatctttacg gtcactgtgc cgaaggcgat
     8401 tgccgcgaag atgagcacgc gcacgaaggc aaataagcca gcctgaacga gaaaagccaa
     8461 cctgcgggtt ggctttttta tacaagggaa ataagaaaag cgagagttac agctctcact
     8521 tatttgttat tactgcgtat ttcctgccag accttatcac aatcggcttt caccgcctga
     8581 tcatttccgg tttgcgttgc acgcagacac tgctgataat ccagtacgcg tacactttcc
     8641 tggtcggcaa actgctgatg ttgtttctct ttcttcagca cgtttaacac gctctggcag
     8701 gcttcaattt tttctggcga accttgtgcg gtgttgatac aggcgctgta tgcctctttc
     8761 agacgactgt cttcctgtgg cgcggttgat tgcgcacaag ctaccagccc cgatgccatt
     8821 atggcgatga gaatcagttt tttcatagca aattcctcaa acgtgcggca ggcacaaaac
     8881 ctgccgcgtc gggcatcaga agatggtgaa tggtgcgatt accataaatt tcacgtcacg
     8941 ctcatcctgg aagatgttac cgtaaccgcc gccccagctt gggatgtcag agtggttgtc
     9001 gtattcggtg aagtgcagtt tgaacatcgt gcctttggcg cgaccgtcct gaatggtgta
     9061 gaccgcatcc aggctgtagg cagactcttc aatagtccgg tttttgtcgt agtacgcatc
     9121 cggattgctc tgccaggtcg caggtttagc atcccatgcg taaacgtagg aagcgccgat
     9181 ggcgaagcct ggaagattcc agtttttcag gtcatacatc gcaccgaaga agaccgcttt
     9241 ttcgccgttg gcgttgaagt cggaacggtt atcccaccag atatccaggc ggccgttgga
     9301 ggaagcgtag gttggagtca tacgttgcag gaagtatccc tgctgaccgt cagccttaac
     9361 ccaggtgcct tcgaggcgca aatccactac gtcagccgcc cggtaaccaa aggtcaacgc
     9421 ttgcagccag gcggtgccgt cataaaggtc gttgacgctg cgatcgtcaa ctttatcgcg
     9481 cgtaccatag aactggtagc tggtggttaa cgggctaccg gcgatatcaa atttgtagct
     9541 ggctttggca aaatattgat cgatataccc ttccgcctga ccaaatgccg cttccagtac
     9601 gaagttattt ttgaagtcgt atttcgcacc gatggagtgc aggtaatcaa ctttggtggt
     9661 tttatcgttc tgataaaact catccatttc cagatgccac ggcgctttgt attcgttggt
     9721 ccacatgtag gagaaactca aggcaccagc atcgccgtaa tcaaaattcg ccccggcttc
     9781 cgccccctga taagtacctg gcataaagct ccagtgcggc gctaacagcg tttgaccagt
     9841 tggctgaatg taacctgccc tcgcccaaac cggaccgtat ttaaatttgg ccgcagcttt
     9901 atacaggctt ataccgcttt tgtcgccgga ccagtcttcg tcataggctt tattactttt
     9961 tgaaaacgcg atttcgttcg ggtgggagct gtcgccgttt tccgccattt caatcgccgt
    10021 aaacgcagca atatcaaggc cgaacatatc agcagcataa ccggactgaa aatcgaggtt
    10081 ggcattccag gtggagtgag aaaggttggt tttgtatttg tcgccgtcgg taacatcttt
    10141 acggtcgcgt tcacgctgcc agtaatatat accgccggtt aaggttgaat cgtcgatgaa
    10201 tccttcggcg cgagcctccg gcattgccat aaagcccgac attgctgtaa caccggcgat
    10261 agccagcgcc agcgtactac gtttgccact aaacgtacgc atgggttaat cctctttgac
    10321 gtataaattg ctgcaccaaa ggtgaacagc gaaaaaataa aaattaaaaa agtagcgttg
    10381 agaaaagtct cgctgactac aggaacagac tatttttcgc gacgcgaatt atcaatctct
    10441 ttctgtaacc agaatgtaag aacatgagca accgcacata
//
LOCUS       DAZGLJ010000159        10267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000159 DAZGLJ010000000
VERSION     DAZGLJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.159"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            209..1741
                     /locus_tag="ACHK49_003547"
     CDS             209..1741
                     /locus_tag="ACHK49_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO9264130.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(1793..2512)
                     /gene="fadR"
                     /locus_tag="ACHK49_003548"
     CDS             complement(1793..2512)
                     /gene="fadR"
                     /locus_tag="ACHK49_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO9264131.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            2734..4275
                     /gene="nhaB"
                     /locus_tag="ACHK49_003549"
     CDS             2734..4275
                     /gene="nhaB"
                     /locus_tag="ACHK49_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO9264132.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4421..4951
                     /gene="dsbB"
                     /locus_tag="ACHK49_003550"
     CDS             4421..4951
                     /gene="dsbB"
                     /locus_tag="ACHK49_003550"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO9264133.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(4997..6265)
                     /gene="umuC"
                     /locus_tag="ACHK49_003551"
     CDS             complement(4997..6265)
                     /gene="umuC"
                     /locus_tag="ACHK49_003551"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO9264134.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(6265..6684)
                     /locus_tag="ACHK49_003552"
     CDS             complement(6265..6684)
                     /locus_tag="ACHK49_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO9264135.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            7057..7968
                     /gene="hlyE"
                     /locus_tag="ACHK49_003553"
     CDS             7057..7968
                     /gene="hlyE"
                     /locus_tag="ACHK49_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFO9264136.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QNFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKRHGKKTLFEVPEV"
     gene            complement(8175..8621)
                     /locus_tag="ACHK49_003554"
     CDS             complement(8175..8621)
                     /locus_tag="ACHK49_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO9264137.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(8713..9372)
                     /locus_tag="ACHK49_003555"
     CDS             complement(8713..9372)
                     /locus_tag="ACHK49_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO9264138.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLATRV
                     L"
     gene            complement(9444..9737)
                     /locus_tag="ACHK49_003556"
     CDS             complement(9444..9737)
                     /locus_tag="ACHK49_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO9264139.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            complement(9749..9907)
                     /locus_tag="ACHK49_003557"
     CDS             complement(9749..9907)
                     /locus_tag="ACHK49_003557"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264140.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            9978..>10267
                     /gene="pliG"
                     /locus_tag="ACHK49_003558"
     CDS             9978..>10267
                     /gene="pliG"
                     /locus_tag="ACHK49_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO9264141.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHG"
BASE COUNT         2636 a         2535 c         2416 g         2680 t
ORIGIN      
        1 actctgcggc aatatggcgt ctttagtatc tcctgctgaa cgcccttttt atccagcata
       61 aaattccgtt cagaagcgga ttagtggcac tctgataata agaaatcgca aagaaaaaaa
      121 ttctccggcg acgcggtttt taacaggtgt tctatgcttg aaatgaggtg ctccacacag
      181 agagcgccga caacaatgag gtgcgcgtat ggcgacgatc gattctatga ataaggacac
      241 cacacgtttg agcgatggac ccgactggac gttcgacctg ctggatgttt atctggcaga
      301 gatagaccgg gtggcgaaac tctaccggct ggatacctac ccgcaccaga ttgaagtgat
      361 aacctcagaa cagatgatgg atgcctactc cagcgtcggc atgccaatta actatccgca
      421 ctggtcattc ggtaaaaagt ttatcgaaac tgaacggctg tataagcacg gtcagcaagg
      481 actggcctat gaaatcgtca ttaactctaa cccgtgtatc gcttacctga tggaagagaa
      541 caccattacc atgcaggcgc tggtgatggc acacgcctgc tatgggcata actctttctt
      601 taaaaacaat tacttattcc gcagttggac cgacgccagt tcgattgtcg attatctgat
      661 ttttgcccgt aaatatatta ccgagtgcga agaacgttat ggcgttgatg aagtagaacg
      721 gcttctggac tcgtgccacg cgctgatgaa ctacggcgtg gaccggtaca aacgcccgca
      781 aaaaatctca ctgcaagaag agaaagcccg gcagaaaagt cgcgaagagt atctacaaag
      841 tcaggtcaat atgctctggc gtaccctgcc gaagcgcgag gaagagaaaa cggttgctga
      901 agcgcgccgc tatccgtccg aaccacaaga aaacctgctc tattttatgg agaaaaatgc
      961 gccactgctg gaatcatggc agcgtgaaat cctgcgtatt gtgcgtaagg tgagccagta
     1021 tttttatccg caaaaacaga ctcaggtgat gaacgaaggc tgggcgacct tctggcacta
     1081 taccatcctt aaccatctgt atgatgaagg gaaagtaacg gaacgtttta tgctggagtt
     1141 tttgcacagc cacaccaatg tggtcttcca gcccccctat aacagcccgt ggtacagcgg
     1201 catcaacccg tatgccctcg ggttcgccat gttccaggat attaaacgga tttgtcagtc
     1261 gccaacggaa gaagacaaat actggttccc ggatatcgcc ggttccgact ggctggaaac
     1321 gctgcatttc gcgatgcgtg atttcaaaga tgagagtttt atcagccagt ttctgtcacc
     1381 gaaagtgatg cgtgatttcc gcttcttcac cgtgctggat gatgatcggc ataattatct
     1441 ggagatttcc gctattcata atgaagaagg ttatcgggag atccgtaacc ggttatcgtc
     1501 gcaatataac ttaagtaatc tggagccgaa tattcagatc tggaacgtgg atttgcgcgg
     1561 cgaccgttcg ctgacgctgc gttacattcc acataatcgc gcaccgctgg atcgggggcg
     1621 caaagaagtg ctgaagcatg tgcatcgcct gtggggattt gatgtgatgc tcgaacagca
     1681 aaacgaagac ggcagcatcg agttgctgga acgttgcccg ccaagaatgg gaaatctgta
     1741 aaaacaacaa aaaacccctc gtttgagggg tttgctcttt aaacggaagg gattatcgcc
     1801 cctgaatggc taaatcaccc ggcagatttt tctgcatccg gtgccaaatc tcgccactct
     1861 catgcccata gcgacgcact gtttcgtaca cctgatcgtg cgcgccttca ctgcacaacg
     1921 ccgacagttt gtggtagaag cccagcgcca gactgcgcgc ttccggattg gcgaaatagt
     1981 gacgaccaat gcgcgtatac agccctttca tcccgttaag gatcagaccg taaatcgggt
     2041 tgccggaagc aaacgccagg ccgcggaata tgttgtaatc cagctcggca aaggcatcgg
     2101 cgtgatcggc cacttcatta gcggtagcca gcacttcctg cgctttatcg ggatgctgac
     2161 gaaacgcggt gcgaataaaa atagtggaaa tattggtacg caccgacagc aaattatcaa
     2221 taagctgcgg cacactttcg tgatccagtc gcgccagtgt ttcaaggata tttaaaccag
     2281 aggtttccca gaaattattc accttcgtcg gcttgccatg ttgaatggtc aaccagccat
     2341 ctcgtgccag acgctgtaac acttcacgta acgtagtacg cgttacgcca attaattctg
     2401 aaagttcacg ttctgcgggc aaaatagtcc cgggagggaa gcggttattc cagatacttt
     2461 caataatgta ctcttccgcg aaacccgccg ggctttgcgc cttaatgacc atagtgagat
     2521 ttccataaca cagcaaaaca aagttggact catcatacca gagggctaac tacgctgata
     2581 gcagacaaaa agaagaaaaa agggatcaag gcttcatttt ccgcatctct ttccccttga
     2641 ctatcaagac ttgcctcact cctgcctgac cgttaagctt tgcgtttaaa cataaaaaca
     2701 tcattgatta tttgtaaggt aagggaaacc atcatggaga tctcctgggg ccgcgcgcta
     2761 tggcgcaatt ttttgggcca gtcccccgac tggtacaaac tcgccctcat cattttctta
     2821 atcgtaaacc cgttaatttt cctcatcagc cctttcgtcg cgggctggtt gctggtagcg
     2881 gaatttattt tcactctggc gatggccctg aaatgctacc cgctgctccc cggtggtctg
     2941 ttggctatcg aagcggtatt catcggcatg accagcgcgg aacacgtccg tgaagaggtg
     3001 gcggcaaatc ttgaagtctt gctgttattg atgtttatgg tggcgggtat ctattttatg
     3061 aaacagctgt tgctgttcat atttacccgt ttgctgttaa gcattcgctc caaaatgctg
     3121 ctgtcgctct ctttttgcgt ggcggcggcg ttcctctccg cgttcctcga tgccttaacc
     3181 gtcgtggcgg tggtgatcag cgttgcagtc ggtttttatg gtatttatca ccgcgtagcc
     3241 tcttcccgta ctgaagatac cgacctgcaa gacgatagtc atatcgacaa gcattacaaa
     3301 gtggttctgg aacagttccg tggctttctg cgtagcctga tgatgcatgc cggtgtcggc
     3361 actgcattag gcggcgtgat gaccatggtg ggcgaaccac agaacctgat catcgctaaa
     3421 gcggctggct ggcattttgg cgatttcttc ctgcgcatgt cgccggtgac cgttccggtt
     3481 ctgatttgtg gcctgttaac ctgcctgctg gtagagaagc tgcgttggtt tggctacggt
     3541 gaaacgctgc cggagaaagt ccgcgaagtg ttgcaacagt ttgacgatca aagccgccac
     3601 cagcgtactc gtcaggataa aatccgtctg attgtccagg cgattattgg cgtctggctg
     3661 gtgactgcgc tggcgttgca tctggcggaa gttggcttga ttggtttgtc agtcattatt
     3721 ctggcgacat cattgaccgg tgtcaccgat gaacatgcta tcggtaaagc cttcaccgaa
     3781 tctctgccat tcaccgcact gttgacggtg tttttctcgg tagtcgcggt gattatcgac
     3841 caacaactgt tttcgccaat tattcagttt gtgttgcagg catcggaaca tgctcagctg
     3901 tcgctgttct atattttcaa cggtctgctg tcatccattt cggataacgt cttcgtgggg
     3961 acgatttata tcaacgaagc gaaagcggca atggaaagtg gcgctatcac gttgaagcaa
     4021 tacgagctgc tggcggtcgc cattaatacc ggtaccaatc tgccctccgt cgctacgccg
     4081 aacggtcagg ctgcgttcct gttcctgctg acctctgcac tcgcgccatt gattcgcctc
     4141 tcttatggcc gcatggtgtg gatggccctg ccttacaccc tcgtactgac actcgtcggc
     4201 ttgctctgcg tcgagtttac gcttgcccct gtaaccgaat ggtttatgca aatgggctgg
     4261 atagcaacgc tttgataaca acttaccggg catataaatg cccggtttgc cttttcgccc
     4321 gataattgtc caattgcgtc ctatttttgc tgcctcctgg tggcgcagcg aatgaattgg
     4381 tttaaactgc gcactctatg catattgcag ggaaatgatt atgttgcgat ttttgaacca
     4441 atgttcacaa ggccggggcg cgtggttgtt gatggcgttt actgctctgg cactggaact
     4501 gacggcgctg tggttccagc atgtgatgtt actgaaacct tgcgtgctct gtatttatga
     4561 acgctgcgcg ttattcggcg ttctgggtgc tgcgctgatt ggcgcgatcg ccccgaaaac
     4621 tccgctgcgt tatgtagcga tggttatctg gttgtatagt gcgttccgcg gtgtgcagtt
     4681 aacttacgag cacaccatgc ttcagctcta tccttcgccg tttgccacct gtgattttat
     4741 ggttcgtttc ccggaatggc tgccgctgga taaatgggtg ccgcaagtgt ttgtcgcctc
     4801 tggcgattgc gccgagcgtc agtgggattt tttaggtatg gaaatgccgc agtggctgct
     4861 cggtattttt atcgcttacc tgattgtcgc ggtgctggtg gtgatttccc agccgtttaa
     4921 agcgaaaaaa cgtgacctgt tcggtcgcta atccattcgg cgctcctgcg ggagcgcttt
     4981 tttcctgccg ctatatttat ttgacccgca gtaaatcaga acttcgcgtt gtataacgtg
     5041 gtgaaagcat ggcacgcttc atctgccatt gttgctggat cccctgcccg gcaaaataga
     5101 gtgttcctct gccctcttta gcattcagtg tatccattac cgtcatcaat tgctcactcc
     5161 cggggcgcgg tgcgttgtca tcgaataaat tgagctgcgc gactccctga ctgaagaaat
     5221 cccccagcat cacgcccgct ttttggtagc gatggcccgc ttgccagatg gcatccagag
     5281 atcgcgtagc agcgttaatg atatccctgc tgtcctgagt gggcgtcagc agttttaccg
     5341 acgcgctatt gccgtaataa ggttcattga gcgcaaatgg tgacgtctta ataaatgtgg
     5401 agataaaccg acaatattga tgctcgctgc gaagtttttc cgccgcccgg gcagcgtaac
     5461 tacaaatggc ctgccgcatc gatggataat ccgtgatgcg ttcaccaaac gagcgggaac
     5521 agataatttc ctgcttcgtc ggtgcaaact cttccagttg caaacagggt tcgccgcgca
     5581 gttcacgcac cgttctttcg agcacgacat taaaatgttt acggataaac cggatatctg
     5641 tatccgccaa atcaagaacg gttttgatcc ccatcgcgtc cagttttttg ctgatccgcc
     5701 gtccaatccc ccagacttca tccacgggga gagcagacat taatttacgc tggcgttcca
     5761 gatttgataa atccaccacc ccccccgtct gccgctgcca tttttttgcc gcatgattgg
     5821 caagcttagc cagcgttttg gtctgggcga tccccacacc aacagtaaga tgggtacgtt
     5881 gtagcaccgt tgcgcgaatt tctctgccaa aatcagtcag atcgcgacaa ttacgcacac
     5941 ctgtcagatc gcagaatgcc tcatcaatac tgtaaatctc gacgcggggc gatagttctt
     6001 ccagcgtcga catcacccga ttgctcatgt ctgcgtaaag ctcataattg ctgctaaagc
     6061 aaaccacacc acagcgacga aacagatctt tttgtttgaa ccagggatcg cccattttaa
     6121 cacccagcgc ctttgcctca gcgtttcggg cgataacgca accgtcatta ttcgatagca
     6181 caaccaccgg tttaccccat aaatcagggc gaaacaccgt ctcacagctg gcataaaacg
     6241 cgtttacatc acagagagca aacatcagcg catcgcctta acgacgtgga tcaccacacc
     6301 aaagacatcc agcgtatctt cgctactgat ggtaatgggc gagtacgcac tgttcatggg
     6361 aataagctgt accgtcgggc gtagttgcaa ttttttcacc gtaaactcgc cgtcaacagc
     6421 agcgatgaca atatcaccat ggctggcggt aatagcgcta tcgacaatca gtaaatcacc
     6481 gtcactaatt ccaccatcaa tcatagaatc accacttgct ttgacgaagt aagtcgcgct
     6541 gggatgctgg atcaacagtt gattcagatc gatgcgctgt tcaacgtaat ctgctgccgg
     6601 tgaaggaaag ccacactgaa caagatcgct aaatagcgga aaagtcacaa tttcgcggag
     6661 atccgcaggc ttgataaaca acataataat ctgcctgaag ctatactgtt tttatataca
     6721 gtagtctgtt cttgccagca gatcaatact gattcaggct atcaatattt gtcgctgcat
     6781 agactgctga tttttcgttc tcttatcctg tgctcacgcg gccttctggc gactacgctc
     6841 atccagcaga aatgaaaaat attacccggc taaaaaatag aatagaagca ttcgccataa
     6901 tgacattaaa cattgtttga tatttatcat attaatagaa ataaagacat tgacgcatcc
     6961 cgcccggcta actatgaatt agatgaagta aaatttatta atagttgtaa aacaggagtt
     7021 tcattacaat ttatatattt aaagaggcga atgattatga ctgaaatcgt tgcagataaa
     7081 acggtagaag tagttaaaaa cgcaatcgaa accgcagatg gagcattaga tctttataat
     7141 aaatatctcg atcaggtcat cccctggcag acctttgatg aaaccataaa agagttaagt
     7201 cgctttaaac aggagtattc acaggcagcc tccgttttag tcggcgatat taaaacctta
     7261 cttatggata gccaggataa gtattttgaa gcaacccaaa cagtgtatga atggtgtggt
     7321 gttgcgacgc aattgctcgc agcgtatatt ttgctatttg atgagtacaa tgagaagaaa
     7381 gcatccgccc agaaagacat tctcattaag gtactggatg acggcatcac gaagctgaat
     7441 gaagcgcaaa aatccctgct ggtaagctca caaagtttca acaacgcttc cgggaaactg
     7501 ctggcgttag atagccagtt aaccaatgat ttttcagaaa aaagcagcta tttccagtca
     7561 caggtagata aaatcaggaa ggaagcgtat gccggtgccg cagccggtgt cgtcgccggt
     7621 ccatttggat taatcatttc ctattctatt gctgcgggcg tagttgaagg gaaactgatt
     7681 ccagaattga agaacaagtt aaaatctgtg cagaatttct ttaccaccct gtctaacacg
     7741 gttaaacaag cgaataaaga tatcgatgcc gccaaattga aattaaccac cgaaatagcc
     7801 gccatcgggg agataaaaac ggaaactgaa acaaccagat tctatgttga ttatgatgat
     7861 ttaatgcttt ctttgctaaa agaagcggca aaaaaaatga ttaacacctg taatgagtat
     7921 cagaaaagac acggtaaaaa gacactcttt gaggtacctg aagtctgata agcgattatt
     7981 ctctccatgt actcaaggta taaggtttat cacatttaaa aacacagacg ttttttaaag
     8041 attgagagtt acctgttgat aataaggtat cgggtaactc tcattatgac agagcgcttt
     8101 caaaaacagg gctacgaaat ggcgagcatc cagacgtata ttaagaaagc ccccatcaac
     8161 atctattaaa tttatcactg cgcccagtca ggtttattta agatatgatc ctgccagtca
     8221 atcacttctg attctttcac tgcgatatga cgcacagaga tacgttcacc atgcattgcc
     8281 gctttcgaac ccgtaagtag cggatgccac gcaggtaaat ctttaccttc cgccagcaac
     8341 cgataagcgc aggtcattgg cagccattcg aatgttggca gattttcacg ggttaattta
     8401 atgcagtcgg gttcaaactc gaaacgacgt tcgtagttcc gacattgaca ggttttaata
     8461 ttgagctggc gacaggcgac gttagtgaag tagatttcgt cggtgtcttc atccatcagt
     8521 ttatgcaggc aacactgacc gcaaccatca cacaacgact cccattccgc atcgctcatt
     8581 tcgtccaggg ttttactttg ccagaaaggt acatcgctca tcaggtgttc cgccattacg
     8641 ttaaaacgca ccttataacc agtctggcac agcgatgcaa gttttgccgc cgctttcagg
     8701 cggcaaaaag tattacaaaa cgcgagttgc caaagaatgc ccatcgaaag tgactgtcag
     8761 ctcatcaccg ctttgcaacg ggccgacgcc atcaggcgtg cctgtcagca caacgtcacc
     8821 ggccttgagg gtaaaaaact tgctcatata agcgatcagc ggaacaattt tatggatcat
     8881 gtccgcagtc gtaccttgct gacgttgctc gccgtttacg ctcaggccca gcgttgtatt
     8941 ttgcgggtcg ccggtaaatt ccgccgcggg aataaacccg gaaagcggac aagagttatc
     9001 aaacgcttta gccttttccc acggctgccc ggctttcttc atttttccct gaacatcacg
     9061 caatgtcaga tcgagcgcca cgccataacc ggcaatggct ttgcggacat gctcttccgt
     9121 agcctgacgc agcgtcgcgc caatcaacac cgccagttcg acttcatgat gaactgaacc
     9181 gaaatcggat gggatcgcca atggctgccg cagatcgcac agtgccgttt ctggtttaat
     9241 aaacagcact ggctcttcgg gcactgcgct gcccatctct ttaatatgtt tggcatagtt
     9301 actgccaaca cagactactt tactcaccgg ataatccagc agcgcacctt gccagttgtg
     9361 atgttgatac ataacgttcc cctcacctta tcgtggatat gaaaacccgc gccgacaaag
     9421 ataatgccac cgccggatat cggttatttg ttagtgtcgt ctgttttctg ccccatgacg
     9481 gaaagatgtt gcttcagcaa atcttcgggt ggtggcggta actgcaaata gtaaccttgc
     9541 tcggttaatg ccagtttaac cttttcaata tcagcattga ccagtttctt ccgcccatcc
     9601 aacggcagaa tcatcgctaa ctgaggctga ccaaaacctt tcatcagttc ctcaggaaca
     9661 cgcgaaaaat cgtctttttt ttcgacatat aaataggtct ggtcacgctt gctgcttcga
     9721 taaatcacac aaaacatact tttactcttt aagatgccag gttttggcat aagctaacat
     9781 caatgaaacc cgcgccacaa gccagccagc tgtagatttc tacggtttgt tgagtccatg
     9841 cccctttata agtaatcccc ccggcaaaaa tgccgcgccg ggattttacc gaacgcagga
     9901 aagccactgg aattctcccg ttagggattg ttatattata acagttcatc gtactcattc
     9961 tgaacaggag actaccaatg aaaatcaaga gcatcagtaa ggctgtattg ctgtttgcac
    10021 tgctaacctc cacgagcttt gctgcgggta agaatgtcaa tgttgagttc agaaaaggcc
    10081 atagcagcgc tcagtactcc ggcgaaataa agggatacga ttacgataca tataccttct
    10141 atgccaaaaa aggtcagaaa gtgcatgtga gtatctctaa tgaaggcgct gatacttacc
    10201 tcttcgggcc aggaatcgat gattcagttg atttatccag atattcccca gaactcgata
    10261 gtcacgg
//
LOCUS       DAZGLJ010000160        10263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000160 DAZGLJ010000000
VERSION     DAZGLJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.160"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..245)
                     /locus_tag="ACHK49_003559"
     CDS             complement(<1..245)
                     /locus_tag="ACHK49_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9264142.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSW"
     gene            complement(277..1293)
                     /gene="lplA"
                     /locus_tag="ACHK49_003560"
     CDS             complement(277..1293)
                     /gene="lplA"
                     /locus_tag="ACHK49_003560"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO9264143.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEVITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            complement(1321..1965)
                     /locus_tag="ACHK49_003561"
     CDS             complement(1321..1965)
                     /locus_tag="ACHK49_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO9264144.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            2071..3039
                     /gene="serB"
                     /locus_tag="ACHK49_003562"
     CDS             2071..3039
                     /gene="serB"
                     /locus_tag="ACHK49_003562"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO9264145.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            3088..4470
                     /gene="radA"
                     /locus_tag="ACHK49_003563"
     CDS             3088..4470
                     /gene="radA"
                     /locus_tag="ACHK49_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO9264146.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            4491..5723
                     /gene="nadR"
                     /locus_tag="ACHK49_003564"
     CDS             4491..5723
                     /gene="nadR"
                     /locus_tag="ACHK49_003564"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO9264147.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(6030..7697)
                     /gene="ettA"
                     /locus_tag="ACHK49_003565"
     CDS             complement(6030..7697)
                     /gene="ettA"
                     /locus_tag="ACHK49_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO9264148.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            7908..9845
                     /gene="sltY"
                     /locus_tag="ACHK49_003566"
     CDS             7908..9845
                     /gene="sltY"
                     /locus_tag="ACHK49_003566"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO9264149.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     HIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSTTEWGRRY"
     gene            9935..10261
                     /gene="trpR"
                     /locus_tag="ACHK49_003567"
     CDS             9935..10261
                     /gene="trpR"
                     /locus_tag="ACHK49_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO9264150.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
BASE COUNT         2312 a         2657 c         2917 g         2377 t
ORIGIN      
        1 atccaggaag ggcatatcag caactctggt ggacaaccat aaatttctgc cattttgctt
       61 ttgatgtttg agctaagccg ttccgtcttt tcgatctgtt ttagagaatt aatcgagata
      121 cctaacattt tacttacttc agcaagcgat aatttatgcc tgatacgcac ggcggctagc
      181 caggaaatac gctggcttaa cattaaattg ataatctccc gctcctggtt ttccagatcg
      241 atcatattcc cttcctttgg tattttaatc agcgagttac cttaccgccc ccgctatcca
      301 cgtcgacaac tcccgtagct ctttttcctg gtccgggaag tcaaccaaca gcgcttcgca
      361 ctcttgttgc agcatatccg cgcggtacag gcagccttgc agtcgcccgg cgagggcttc
      421 cagcggcgcg gggttgaggc tgtcggtaaa cacctgggcg cgggtgatat ggcctttttc
      481 aacgtcgaaa tgcagttcca cgccgcccca gctaaagcgt tcatccagca gatgcgagaa
      541 tgccggagcc tgaccgaagt tccattccca gctactctga cgggcaaagg tttcggcgaa
      601 gtttggcaag tctggcgttt tgtccgggga gatgatttcc gcttctacac gctcgccata
      661 atgggcgaaa aaggccttgg ttatgacctc gcaaacctgc tcatgggtga tccccggcag
      721 cagctcggtg aggttggtca cgcgggaacg cactgaggta atgcctttcg ccgccagttt
      781 ctttttatcc ggattgagat agtttgccag gcggctaagg tcggcattga gcagcaaagt
      841 gccgtggtgg aagccacgat ctttggtttc gcgataggcc gagcctgaga ctttgcggtc
      901 gccttcgact gttttcacca ccagatcgtt acgcccggac gcttcggcgc tgacgccgag
      961 cgcgttcagc gcattgagca caatcgacgt agagatggtt ttatcgtact ccggcttgcc
     1021 agccataaag gtaaagcagg tattgccgag atcgtggaac accgcgccgc caccgctact
     1081 gcgccgcgcc aggcggacgt tatcttcttc catccgccgg gtattacact ctttccacgg
     1141 gttctgcgcg cgaccaatca ctaccgtgtc ggcattgcgc cagagaaaca gaacgcgctg
     1201 cgtggcgggc atttggcgaa aaatacactc ttccaccgcc aggttaaacc acgggtcgta
     1261 agagtcagag atgagcaggc gtaatgtgga cataacgatt tcctttcttg tagtaatggg
     1321 tcactctttt ttctcgcttt cttcctcttc cggcaccggt ttgctggcgg ttaacaggaa
     1381 gggcgattgc tgccagcggg tgcgtttacc ctgtagcagc gtgcgggtca gcactacgcc
     1441 gattgccagt gagagcagca acatcaggcg taaaatgtta gtggtgttat ccacctgttg
     1501 ggcttcggtg gcgagcgtat gggtgtcgag tgtcaggcgc agatagccga gcggtccgtt
     1561 tttacccgca attggctcga caatctgctg gttaaaatag ccgcctgctt ttttaccgtc
     1621 gagcgccagc cggtcgcgca cttcgacgct ttcgcctgaa cgggcgataa gatcgccttg
     1681 ttcgtcatac acacccgcgt cgaggatgcg gctttcatcc gttaactgat cgaggatcgc
     1741 ctgaatgcgt ttttcatctg gtgagtcggt acgcatcagc ggtgcaacgt tcagcgtcac
     1801 ctgacgcgcc agggtgcggg ccagttcttc cagctgtgga ttacgctgtc gctggtggtt
     1861 ttgactaaac catgacgccc cctgcatcag tgccactaac aaggcaagac agaacaggac
     1921 aatcactgcc cgatgcagcc ggaatttcag ttttgtgcga gccatcttcc accctttgaa
     1981 aatttgagac ttaatgttgc cagaagcaat ggatacaagg tagcctcatg cgttattttc
     2041 cctgcttcga acgattttac aggagcctta atgcctaaca ttacctggtg cgacctgcct
     2101 gaagatgtct ctttatggcc gggtctgcct ctttcattaa gtggtgatga agtgatgcca
     2161 ctggattacc acgcaggtcg tagcggctgg ctgctgtatg gtcgtgggct ggataaacaa
     2221 cgcctgaccc aataccagag caaactgggc gcggcgatgg tgattgttgc cgcctggtgc
     2281 gtggaagatt atcaggtgat tcgtctggca ggttcactca ccgcgcgcgc tacgcgcctg
     2341 gcccatgaag cgcaactgga tgtcgcgccg ctggggaaaa tcccgcacct gcgcacgccg
     2401 ggtttgctgg tgatggacat ggattccacc gccatccaga ttgaatgtat tgatgaaatt
     2461 gccaaactgg ccggaacggg cgagatggtg gcggaggtaa ccgaacgggc gatgcgcggc
     2521 gaactcgatt ttaccgccag cctgcgcagc cgcgtggcga cgctgaaagg cgctgatgcc
     2581 aatattctgc aacaggtgcg tgaaaatctg ccgctgatgc caggcttaac gcagctggtg
     2641 ctcaagctgg aaacgctggg ctggaaagtg gcgattgcct ccggcggctt tactttcttt
     2701 gctgaatacc tgcgcgacaa gctgcgcctg accgccgtgg tagccaatga actggagatc
     2761 atggacggta aatttaccgg caatgtgatc ggcgacatcg ttgacgcgca gtacaaagcg
     2821 aaaactctga cccgcctcgc gcaggagtat gaaatcccgc tggcgcagac cgtggcgatt
     2881 ggcgatggag ccaatgacct gccgatgatc aaagcggcag ggctggggat tgcctaccat
     2941 gccaagccaa aagtgaatga aaagacggaa gtcaccatcc gtcacgctga cctgatgggg
     3001 gtattctgca tcctctctgg cagcctgaat cagaagtaat tgcctgcccg tcatcctgcg
     3061 ggcggcacag cattaacgag gtacaccgtg gcaaaagctc caaaacgcgc ctttgtttgt
     3121 aatgaatgcg gggccgatta tccgcgctgg caggggcagt gcagtgcctg tcatgcctgg
     3181 aacaccatca ccgaggtgcg tcttgctgcg tcgccaacgg tggcgcgtaa cgagcgtctc
     3241 agtggctatg ccggtagcgc cggggtggca aaagtccaga aactctccga catcagcctt
     3301 gaagagctgc cgcgtttttc caccggattt aaagagttcg accgcgtatt aggcggcggc
     3361 gtggtaccgg gaagtgccat tctgattggc ggtaacccag gcgcggggaa atccacgctg
     3421 ttgctgcaaa cgctgtgcaa actggcccag cagatgaaaa cgctgtatgt caccggcgaa
     3481 gagtcgctgc aacaggtggc aatgcgcgct catcgccttg gcctgccgac tgacaatctc
     3541 aatatgttgt cggaaaccag catcgagcag atctgcctga ttgctgaaga agagcaaccg
     3601 aagctgatgg taattgactc catccaggtg atgcatatgg cggatgtaca gtcatcgcct
     3661 ggtagcgtgg cgcaggtgcg tgaaacggcg gcttatttga cacgcttcgc caaaacgcgc
     3721 ggtgtggcga ttgtcatggt tggacacgta accaaagatg gctcgctggc gggccctaaa
     3781 gtgctggaac actgtatcga ctgttcggtg cttttggatg gcgatgccga ctcccgtttt
     3841 cgcaccttgc gcagccataa aaaccgcttc ggcgcggtga atgagctggg cgtcttcgcg
     3901 atgaccgaac aggggctgcg tgaagtcagc aacccttcgg caattttctt aagtcgcgga
     3961 gatgaagtga cctccggcag ctccgtgatg gtggtgtggg aaggaacgcg tccgttgctg
     4021 gtggagattc aggcgctggt cgatcactcg atgatggcga atccgcgccg cgtggcggta
     4081 ggtctggagc aaaaccgtct ggcaatcctg ctggctgtct tgcaccgtca cggtggtctg
     4141 caaatggccg atcaggatgt atttgtgaac gtggtcggtg gtgtgaaggt aaccgaaacc
     4201 agtgccgact tagcgttact gctggcgatg gtttccagcc tgcgtgacag accgctaccg
     4261 caggatctgg tggtgtttgg tgaagtcggg ctggcagggg agatccgccc ggtgcccagc
     4321 ggtcaggagc gaatctctga agcggcgaaa cacggttttc gccgggcgat tgttccggcg
     4381 gctaacgtgc cgaaaaaagc gccggaaggg atgcagattt ttggcgttaa aaaactctcc
     4441 gacgcgctta gcgtgttcga cgacttataa tgagagatac ggagggagat atgtcgtcat
     4501 ttgattacct gaaaactgcc atcaagcaac agggctgcac gttacagcag gtagctgatg
     4561 ccagcggtat gaccaaaggg tatttaagcc agttactgaa tgccaaaatt aaaagcccca
     4621 gcgcgcaaaa gctggaggcg ttgcaccgtt ttttggggct tgaatttccc cggcagaaga
     4681 aaaccattgg tgtcgtattc ggtaagttct acccgctgca taccggacat atctacctta
     4741 tccagcgcgc ctgtagccag gttgacgagc tgcatatcat tatgggtttt gacgataccc
     4801 gcgaccgcgc gttgtttgaa gacagcgcca tgtcgcagca gcccacggtg ccggatcgtc
     4861 tgcgctggtt attgcaaact tttaaatatc agaaaaatat tcgcattcat gctttcaacg
     4921 aagagggcat ggagccgtat ccgcatggct gggatgtgtg gagcaacggc atcaaaaagt
     4981 ttatggctga aaaggggatc cagccggacc tgatctacac ctcggaagaa gccgatgcgc
     5041 cacagtatat ggaacatctg gggatcgaga cggtactggt cgatccgaaa cgtaccttta
     5101 tgagtatcag cggtgcgcag atccgcgaaa acccgttccg ctactgggaa tatattccta
     5161 cggaagtgaa gccgttcttt gtgcgtaccg tggcgatcct tggcggcgag tcgagcggta
     5221 aatccaccct ggtaaacaaa cttgccaata tcttcaatac caccagtgcg tgggaatatg
     5281 gtcgcgatta tgtcttttca cacctcggcg gtgatgagat cgcattgcag tattccgact
     5341 acgataaaat cgcgctgggc cacgcacaat acattgattt tgcagtgaaa tacgccaata
     5401 aagtggcgtt tattgacact gattttgtca ctacccaggc gttctgcaaa aagtacgaag
     5461 ggcgtgagca tccgttcgtg caggcgttga ttgatgaata ccgtttcgat ctggtgatcc
     5521 tgctggagaa caacacgccg tgggtggcgg atggtttacg cagcctcggc agttcggtgg
     5581 atcgcaaaga gttccagaac ttgctggtgg agatgctgga agagaacaat atcgaattcg
     5641 tgcgggttga agaggacgat tatgacagcc gtttcctgcg ctgcgtggag ctggtgcggg
     5701 agatgatggg ggagcagaga taaccgcgat gaaacggctc aaaggcgagg tataaaataa
     5761 gttttttgat gagttgaaaa cctttctgga agaggctgtc gagattaaac aaggtttgaa
     5821 aaaacctgca cgggtgaccc gctacgaaat tgcggatact aaggctgttg taggccggaa
     5881 aaggcgttca cgccgtatcc ggcattcggt gctcaatgcc tgatgcgacg cttaccgcgt
     5941 cttatcaggc ctggagattc acaacgtatt gaaatttctt ggtttgtagg ccggataagg
     6001 cgttcacgcc gcatccggca ttttacgcat tacttcgcaa tacgcttgta cttgatacgc
     6061 ttcggctcca gtgcgtctgc gcccagcgtg cgtttcttgt actcttcgta ctcagtaaag
     6121 ttaccttcga agaactcaac tttaccttca tcctggtagt ccaggatgtg cgtggcgata
     6181 cggtcgagga accaacggtc gtgcgagata accatcgcac agcccgggaa ctccagcagg
     6241 gcgttttcca gcgcgcgcag ggtttcgata tccaggtcgt tggttggttc gtcgagcagc
     6301 agcatgttgc cgccaacctg cagcagcttc gccagatgca gacgaccgcg ctcaccaccg
     6361 gaaagttcac caacgcgttt accctgatca acccctttaa agttaaagcg gccaacgtag
     6421 gcgcggcttg gcatctcggt gttgccgatc ttcataatat ccagcccgcc ggaaacttct
     6481 tcccaaacgg ttttgctgtt atccattgag tcacggaact gatcaaccga tgccagtttc
     6541 accgtttcac ctaaagtgat ggtgccgctg tccggctgtt cctgaccaga gatcatacgg
     6601 aacagggtcg atttacccgc gccgttcgga ccgatgatcc cgacgattgc ccctttcggg
     6661 atcgagaagc tcaggtcatc aatcagcagg cgatcaccgt aggatttacg caggttgctg
     6721 acttccagca ctttatcgcc cagacgcggt ccaggtggaa taaacagttc gttggtttcg
     6781 ttacgtttct gatattcggt gctgttcagc tcttcaaagc gtgccagacg tgctttacct
     6841 ttcgactggc ggcctttagt tccctgacgt acccactcca gctctttctc gatcgactta
     6901 cgacgcgccg cttcttgtga agcttcctgc gccaggcgct gatctttctg ctccagccag
     6961 gaggagtagt tgccttccca cggaatacct tcaccgcggt caagttcgag gatccagcct
     7021 gcaacgttat cgaggaagta acggtcgtgg gtaatcgcca ccacggtgcc ctcgaagtcg
     7081 tgcaggaagc gttccagcca ggccacggat tcggcatcca ggtggttggt cggttcgtcg
     7141 agcagcagca tgtctggttt ttccagcagc aggcggcaca acgctacgcg acgacgctca
     7201 ccaccggaga ggttagcgat tttcgcgtcc cagtccggca gacgtagcgc atccgccgca
     7261 cgctccagct gtacgttcag gttatgaccg tcgtgagcct gaatgatctc ttccagacgg
     7321 ccttgttcag cggccagctt gtcaaaatcg gcatccggat cggcgtacag cgcatacact
     7381 tcatccaggc gtttcagggc gttaaccact tcagaaaccg cttcttcaat ggactcacgc
     7441 acggtgtgtt ccgggttcag ctgcggttcc tgcggcaggt aaccaatctt gatgtctggc
     7501 tgcggacgcg cttcaccttc gatgtcttta tcaatgcccg ccataatgcg cagcagggta
     7561 gacttacccg cgccgttcag acccaggaca ccaatttttg ccccagggaa gaaactcaga
     7621 gagatgtttt tcaaaatatg acgtttcggc ggaacaactt tgccgacacg atgcatggta
     7681 taaacgaatt gagccacgtt ggacttcgcc tctatgttta tcgtgatgat gagttttcaa
     7741 aggcgaagtg tagccttttt ccccgcctaa tcccagttgg tcgatcacct tcgcagtaaa
     7801 agtaaaaaag tgtccgtaac gtggcgtaaa cggcaatgac tggttagcat aaatctatta
     7861 cgcggcatga cgctgcattg atgtatttac acttagagga tgcgcttgtg gaaaaagcca
     7921 aacaagttac ctggcggctg ttggctgccg gtgtctgtct gctgacggtc agcagcgtgg
     7981 cgcgagccga ctcactggat gagcagcgta gccgttacgc gcaaatcaag caggcctggg
     8041 ataatcgaca aatggatgtg gtcgaacaaa tgatgcctgg actgaaggat tatccgcttt
     8101 atccctacct ggaataccgc cagatcaccg atgatctgat gaatcaaccg gcggtgacgg
     8161 tcactaactt tgttcgcgct aaccccacgc ttcctcccgc tcgcacgctg caatctcgtt
     8221 tcgtcaatga actggcgcgg cgtgaagact ggcgtggctt gttagccttt agcccggaaa
     8281 agcccggaac taccgaagcg caatgtaatt actactatgc gaaatggaac accgggcaga
     8341 gtgaagaagc ctggcaaggg gcgaaagagc tgtggctaac cggcaagagc cagcctaacg
     8401 cctgtgacaa gttatttagc gtctggcgtg cgtcaggtaa acaagatccg ctggcttatt
     8461 tagagcgtat ccgtctggcg atgaaagcgg gtaacactgg cctggtaaca gtgctggcag
     8521 ggcagatgcc tgccgattac cagactatcg cctcggcaat catttcactg gcgaacaacc
     8581 ctaatacggt actgaccttc gcgcgtacaa ccggagcgac cgattttacc cgtcaaatgg
     8641 cggcggtggc gtttgccagt gtggcgcggc aggatgctga aaatgcacgg ctgatgatcc
     8701 cgtcgcttgc ccaggcgcag cagcttaatg aagatcatat tcaggagctg cgcgatatcg
     8761 tcgcctggcg tttgatgggc aacgatgtca ccgacgagca ggcgaaatgg cgcgatgacg
     8821 ccattatgcg ctcgcaatcc acgtcgttga ttgaacgccg tgtgcgaatg gcacttggca
     8881 ccggcgatcg tcgcggcctg aatacctggc tggcgcgtct gccgatggaa gcaaaagaga
     8941 aagatgaatg gcgttactgg caggcggatt tattgctgga acgcggacgt gaagctgaag
     9001 cgaaagagat tttgcatcaa ctcatgcaac agcgtggttt ctacccgatg gttgcagcac
     9061 aacgcatcgg agaagagtat gagctgaaga ttgataaagc gccgcagaat gttgacagcg
     9121 ccctgactca ggggccggag atggcgcgcg tgcgcgagtt gatgtactgg aatctcgata
     9181 acaccgcgcg tagcgagtgg gccaatctgg tgaagagcaa gtcaaaaaca gagcaggctc
     9241 aactggctcg gtatgctttc aataaccaat ggtgggatct tagcgttcag gcaacgatcg
     9301 ccgggaagct ttgggatcat ctggaagagc gattcccgct ggcttacaac gatcttttca
     9361 aacgttacac cagtgggaaa gagatcccgc aaagctatgc gatggcgatt gcccgtcagg
     9421 agagcgcctg gaatccgaaa gtgaaatcac cggtaggggc cagcggcctg atgcagatta
     9481 tgcctggtac agcgacccat acggtgaaga tgttctctat tcctggttat agcagccctg
     9541 ggcaattgct ggatccggaa acgaatatca acattggcac cagttacctg caatatgttt
     9601 atcagcagtt tggcaataac cgtattttct cctcagcagc ttataacgcc ggacctggac
     9661 gggtgcgaac ctggcttggc aacagcgccg ggcgtatcga cgctgtggca tttgtcgaga
     9721 gtattccgtt ctccgagacg cgcggttatg tgaagaacgt gctggcttat gacgcttact
     9781 accgctattt catgggggat aaaccgacgt tgatgagcac cacggaatgg ggacgtcgtt
     9841 actgatccgc acgtttatga tatgctatcg tactctttag cgagtacaac cgggggaggc
     9901 attttgcttc ccccgctaac aatggcgaca tattatggcc caacaatcac cctattcagc
     9961 agcgatggca gaacagcgtc accaggagtg gttacgtttt gtcgacctgc ttaagaatgc
    10021 ctaccaaaac gatctccatt taccgttgtt aaacctgatg ctgacgccag atgagcgcga
    10081 agcgttgggg actcgcgtgc gtattgtcga agagctgttg cgcggcgaaa tgagccagcg
    10141 tgagttaaaa aatgaactcg gcgcgggcat cgcgacgatt acgcgtggat ctaacagcct
    10201 gaaagccgcg cccgttgagc tgcgccagtg gctggaagag gtgttgctga aaagcgattg
    10261 att
//
LOCUS       DAZGLJ010000161        10200 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000161 DAZGLJ010000000
VERSION     DAZGLJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.161"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(114..2687)
                     /gene="clpB"
                     /locus_tag="ACHK49_003568"
     CDS             complement(114..2687)
                     /gene="clpB"
                     /locus_tag="ACHK49_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO9264151.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2817..3548)
                     /gene="yfiH"
                     /locus_tag="ACHK49_003569"
     CDS             complement(2817..3548)
                     /gene="yfiH"
                     /locus_tag="ACHK49_003569"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO9264152.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3545..4525)
                     /gene="rluD"
                     /locus_tag="ACHK49_003570"
     CDS             complement(3545..4525)
                     /gene="rluD"
                     /locus_tag="ACHK49_003570"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO9264153.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGELVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4660..5397
                     /gene="bamD"
                     /locus_tag="ACHK49_003571"
     CDS             4660..5397
                     /gene="bamD"
                     /locus_tag="ACHK49_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO9264154.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5668..6009
                     /gene="raiA"
                     /locus_tag="ACHK49_003572"
     CDS             5668..6009
                     /gene="raiA"
                     /locus_tag="ACHK49_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO9264155.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6112..6237
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6113..6160
                     /gene="pheL"
                     /locus_tag="ACHK49_003573"
     CDS             6113..6160
                     /gene="pheL"
                     /locus_tag="ACHK49_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO9264156.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6259..7419
                     /gene="pheA"
                     /locus_tag="ACHK49_003574"
     CDS             6259..7419
                     /gene="pheA"
                     /locus_tag="ACHK49_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO9264157.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7462..8583)
                     /gene="tyrA"
                     /locus_tag="ACHK49_003575"
     CDS             complement(7462..8583)
                     /gene="tyrA"
                     /locus_tag="ACHK49_003575"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO9264158.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8594..9664)
                     /gene="aroF"
                     /locus_tag="ACHK49_003576"
     CDS             complement(8594..9664)
                     /gene="aroF"
                     /locus_tag="ACHK49_003576"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO9264159.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9874..>10200
                     /gene="yfiL"
                     /locus_tag="ACHK49_003577"
     CDS             9874..>10200
                     /gene="yfiL"
                     /locus_tag="ACHK49_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO9264160.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQK"
BASE COUNT         2618 a         2701 c         2387 g         2494 t
ORIGIN      
        1 gaaaaatata aaaactttat cgttcgctca tttttcaggc aaagcatctt aaatatagtc
       61 ttttccgtct aacttataga caaaaacgag ccccgaaggg ctcgttttat catttactgg
      121 acggcgacaa tccggtcttc attaacttcc aggcgaatca ctttacccgg aaccaattca
      181 ccagacagta tttgctgtgc cagcgggttt tcgatctgct gctggatagc acgtttcaaa
      241 ggacgtgcac catagactgg atcgtaaccg ttctcgctca gcagtttcag cgcctcgtca
      301 gaaatgtgga tttcataacc acgttcttcc agacgtttgt acagacgttt caactgaatc
      361 tgcgcaatcg aggcaatgtg ctgttcaccc agcggatgga agaccaccac ttcatcaata
      421 cggttaatga attccggacg gaagttatgg cttaccacac cgagcaccag ctctttcatg
      481 tgcgcataat ccagttcacc gaagcgttcc tgaatcaaat cggaaccgag gttagaggtc
      541 ataatgacaa ccgtattacg gaagtcgacc gttctcccct gcccgtcagt cagacgacca
      601 tcatccagta cctgcaacag aatgttgaag acatccggat gcgctttttc cacttcatcc
      661 agcaggatga cggaatacgg acgacgacgc acagcttcgg tcaggtagcc accttcttca
      721 taaccgacat atcccggagg cgcaccaacc agacgagaca ccgagtgttt ctccataaac
      781 tcggacatat cgatacggac catcgcctcg tcgctatcaa acataaagtt cgccagcgcc
      841 ttacaaagct cagttttccc cacacccgtt gggccgagga acaggaatga accaatcggg
      901 cgatttggat ccgccaaccc cgcacggcta cggcgaatag cgttagatac cgcatcaacc
      961 gcttcgttct gaccaatgac gcgatggtgc agttcttgct ccatacgcag cagtttttcg
     1021 cgctcgcttt ccatcatgcg agaaaccgga atccccgtcc aacgcgccag cacttcagca
     1081 atttcggcgt cggtcacttt attacgcaac agacgcatag ttttgccttc gagctgcgtt
     1141 gcggcttcca gttgcttttc cagttccggg attttgccgt attgcagttc agacatccgc
     1201 gccaggtccc ccacacggcg agcctgttca atagcgattt tcgcctgttc cagttccgct
     1261 ttaatggtct gcgtaccaga aagcgatgcc ttctctgctt tccactcttc ttctaactcg
     1321 gagtactgac gttctttgtc gctcagttct tcgttgagca tatccaggcg ttttttactg
     1381 gcttcatcag actctttcat taacgcctgt tgttccagtt tgagctggat gatacgacga
     1441 tcgagtcggt cgagttcttc tggttttgag tcaatctgca tacgaatgct ggatgctgct
     1501 tcatcgatca ggtcgatggc tttatccggc agctgacggt cagcaatgta gcgatgagac
     1561 aacgtcgccg ctgcaacaat tgccgggtca gtaatttgca catggtggtg caattcgtaa
     1621 cgttctttca ggccacgcag aatcgcaatg gtatcttcaa cagaaggctc ggcaacaaac
     1681 actttctgga aacgacgttc cagcgcagca tctttttcga tgtactggcg atattcgtca
     1741 agcgtcgtgg cacctacgca gtgcaattca ccacgcgcca gcgccggttt cagcatgttt
     1801 ccggcgtcca ttgcgccatc ggctttaccc gcgccgacca tggtatgtaa ttcgtcgata
     1861 aacaggatga cgttgccttc ctgtttggca agatcgttaa gcacgccttt taaacgttct
     1921 tcaaactcac cgcgatattt cgccccagcc accagcgcgc ccatatccag cgccagtacg
     1981 cggcggcctt tcaatccttc cggcacttcg ccgttgataa tacgctgcgc cagaccttca
     2041 acgatggcag ttttaccgac gccgggttca ccaatcagta ccgggttatt tttagtacga
     2101 cgttgcagca cctgaatggt acggcgaatt tcttcatcac gaccaatcac cggatcgagt
     2161 ttgccctgtt cggctcgttc ggtaaggtcg atggtatatt ttttcaaagc ctgacgttgg
     2221 tcttcagcac cttgatcgtt cacgctttca cctccacgca tttgttcaat cgcttgagta
     2281 atgttggcgg tggtcgcccc tgctgctttc aggatgtcgg ccagcgtgcc gcgagactca
     2341 agtgccgcca gaacgaacag ttctgacgag ataaagttat caccacgttt ttgcgccagc
     2401 ttgtcgcaaa gattaagaac gcgcaccaga tcctgtgatg gctggacatc accaccagta
     2461 ccttcaacct gcggtaaacg atttaatgcc tgattgatat ctgtgcgcaa ctggccagca
     2521 tttatgccag cggatgttaa taaaggacta accgaacccc cttcctgatt cagcagggcg
     2581 ctcattaaat gaagtggttc gataaattgg ttgtcgtgcc cgagtgcaag tgattgggca
     2641 tcggcaagag caagctggaa tttattagta agacgatcca gacgcataac tcctcccata
     2701 acggatcaaa gttgctactg gagattaaat gaggtcatcc ctcaattatt caaggttatt
     2761 gaccagatta atgtgaaaag aaaatcacgc gtaccggatc gtcttgattc tttaggttat
     2821 atcagccaaa tgaaacttgc catacgaccg gtggtcttgt cgcgacgata agagaagaaa
     2881 gtctcatttt ccgtatatgt acaacggtcg ccgccgaaaa tttgctcaac acccacgttc
     2941 gccagacgct gccgggcaag ctgataaata tccgccagat acttatcacc atgctgaatg
     3001 aaagctgcac ttgctttagc gtctactgcc ataaacgcct cgcgaacctc cacccccact
     3061 tcgaacgcgc gtggaccaat tgccggccct aaccaggcga gaatattttc cggattatca
     3121 gcaaaacagg aaaccgtctc ttccagcacg cctgcgcaca gtccacgcca gccagcatga
     3181 gcggcggcga cttccgttcc cgctcgattg caaaacagca cagggaggca gtcggcagtc
     3241 atcactgcgc aaaccgtgcc gggcgtattg ctataagagg catctgctcg ttttgaggca
     3301 taaggttcgc cagtaagctt aagcacatct ttgccgtgta cctgctcaag ccagaccggt
     3361 ttagaaggca aattgcccgc agcaaaaagt cgcttgcgat tctcctcaac gtgatccggg
     3421 ttatcgccac aatgggcacc gaggttgagc gagtcatacg ggggcaagct cacgccgccg
     3481 atacgagaag agctacaggc cgcaacacct tttggtagcg gccactgcgg gacaatcagc
     3541 ttactcataa ccagtccact tcatccttat gctcttcgaa atcggcgcgc atcacctcaa
     3601 tcagctccac catatcttgt ggaataggcg catgccattc catttcgatg ccggagatcg
     3661 ggtgataaag acgcagcatg gttgcatgaa gcgcctggcg gtcaaactta cgcagcatgg
     3721 agataaatgc ttccgaagca ccttttggcg gacgcggacg gccaccataa accggatcgc
     3781 ccaccagcgg atgagtgata tgggccatat gcacgcggat ctggtgcgta cgtccagttt
     3841 ccagacgcaa ccgcagacgc gtgtgcacac ggaagtgttc catgatgcga tagtgagtca
     3901 ccgccggttt gcccatcgga tgcaccgcca tgtgagtacg tttggtcggg tggcggctga
     3961 tgggttcatc tactgtaccg ccagcggtca tatgaccaat cgccaccgct tcatactcac
     4021 gagtaatttc acgccgttgc aaagattcga ctaaacgcgt ctgagccgga acggtttttg
     4081 ccacaaccat caggccagtg gtgtctttat ccagacgatg gacgatgccc gcacgtggta
     4141 catcggcaat gggcggatag taatgaagca acgcattcag taccgtgcca tccgggttac
     4201 ccgcgccagg atgtaccacc aggtcgcgcg gtttattaat gacgatgata tcttcatctt
     4261 catagacgat atccagcggg atatcctgcg gttcaaaacg cgcttcttct tcaatctcag
     4321 cattgatggc aaccagctcg ccacccaata ctttttcttt cggcttatca caaactttgc
     4381 cgtttaccag cacgcgctgg tcgaggatcc attcttttat tcgcgaacgt gaataatccg
     4441 ggaacatttc ggccaaagcc tgatctaagc gttgaccgag ttggttttcg gacaccgttg
     4501 cagtgagctg tactcgttgt gccatatata ctgcttcttc gtttatcgtt ggggttttac
     4561 ggctttgccg tttaatatag tgtgctattg tagctggtct taaccgggag caggaacaga
     4621 gaatctcccg tattacattt tgaggaaagt caaaacgtca tgacgcgcat gaaatatctg
     4681 gtggcagccg ccacactaag cctgtttttg gcgggttgct cggggtcaaa ggaagaagta
     4741 cctgataatc cgccaaatga aatttacgcg actgcacaac aaaagctgca ggacggtaac
     4801 tggagacagg caataacgca actggaagcg ttagataatc gctatccgtt tggtccgtat
     4861 tcgcagcagg tgcagctgga tctcatctac gcctactata aaaacgccga tttgccgtta
     4921 gcgcaggctg ccatcgatcg ttttattcgc cttaacccga cccatccgaa tatcgattat
     4981 gtcatgtaca tgcgtggcct gaccaatatg gcgctggatg acagtgcgct gcaagggttc
     5041 tttggcgttg accgtagcga tcgcgatcct caacatgcac gagctgcgtt tagtgacttt
     5101 tccaaactgg tgcgcggcta tccaaacagt cagtacacca ccgatgccac caaacgtctg
     5161 gtattcctga aagatcgtct ggcgaaatat gaatactccg tggccgagta ctatacagaa
     5221 cgtggcgcat gggttgccgt cgttaaccgc gtagaaggaa tgttgcgcga ctacccggat
     5281 acccaggcta cgcgtgatgc gctgccgctg atggaaaatg cataccgtca gatgcagatg
     5341 aatgcgcaag ctgaaaaagt agcgaaaatc atcgccgcaa acagcagcaa tacataacag
     5401 aaacctgaaa tacaaaacgg cagcccttga gctgccgttt ttttattctg ttagttgtga
     5461 aactgaagcg atttagtcac tatcgatctc atcaaatatg gctcgctttg agatattcct
     5521 caagtaaaaa aacacctctt cctgcgattt ctcacaaaaa agattcgttg acaaaaagtg
     5581 acaaaattat gagattccca tcacacattt tgacatcagg agcggtatgc tgaattcacc
     5641 aagacgggaa gacaagaggt aaaatttatg acaatgaaca ttaccagcaa acaaatggaa
     5701 attactccgg ccatccgcca acatgtcgca gaccgtctcg ccaaactgga aaaatggcaa
     5761 acacatctga ttaatccaca tatcattctg tccaaagagc cacaagggtt tgttgctgac
     5821 gccacaatca atacacctaa cggcgttctg gttgccagtg gtaaacatga agatatgtac
     5881 actgcaatta acgaattgat caacaagctg gaacggcagc tcaataaact gcagcacaaa
     5941 ggcgaagcac gtcgtgccgc aacatcggtg aaagacgcca acttcgtcga agaagttgaa
     6001 gaagagtagt cctttatatt gagtgtatcg ccaacgcgcc ttcgggcgcg ttttttgttg
     6061 acagcgtgaa aacagtacgg gtactgtact aaagtcactt aaggaaacaa acatgaaaca
     6121 cacaccgttt ttcttcgcat tcttttttac cttcccctga atgggaggcg tttcgtcgtg
     6181 tgaaacagaa tgcgaagacg aacaataagg cctcccaaat cggggggcct tttttattga
     6241 taacaaaaag gcaacactat gacatcggaa aacccgttac tggcgctgcg agagaaaatc
     6301 agcgcgctgg atgaaaaatt attagcatta ctggcagagc ggcgcgaatt ggccgtcgag
     6361 gtaggaaaag ccaaactgct ctcgcatcgc ccggtacgag atattgatcg tgaacgcgat
     6421 ttactggaaa gattaattac gctcggtaaa gcgcaccatc tggacgccca ttacattact
     6481 cgcctgttcc agctcatcat tgaagattcc gtattaactc agcaggcttt gctccagcaa
     6541 catctcaata aaattaatcc gcactcagca cgcatcgctt ttctcggccc caaaggttct
     6601 tattcccatc ttgcggcgcg ccagtatgct gcccgtcact ttgagcaatt cattgaaagt
     6661 ggctgcgcca aatttgccga tatttttaat caggtggaaa ccggccaggc cgactatgcc
     6721 gtcgtaccga ttgaaaatac cagctccggt gccataaacg acgtttacga tctgctgcaa
     6781 cataccagct tgtcgattgt tggcgagatg acgttaacta tcgaccattg tttgttggtc
     6841 tccggcacga ctgatttatc cactattaat acggtctaca gccatccgca gccattccag
     6901 caatgcagca aattccttaa tcgttatcct cactggaaga ttgaatatac cgaaagtacg
     6961 tctgcggcaa tggaaaaggt tgcacaggca aaatcaccgc atgttgctgc gttgggaagc
     7021 gaagctggcg gcactttgta cggtttgcag gtactggagc gtattgaagc gaatcagcaa
     7081 caaaacttca cccgatttgt ggtgttggca cgtaaagcca ttaacgtgtc tgaccaggtt
     7141 ccggcgaaaa cgacgttgtt aatggcgacc gggcaacaag ccggtgcgct ggttgaagcg
     7201 ttgctggtgc tgcgcaacca caatctgatt atgacccgtc tggaatcacg cccgattcac
     7261 ggtaatccat gggaagagat gttttatctg gatattcagg ccaatcttga atcagcggaa
     7321 atgcaaaaag cattgaaaga gttaggggaa attacccgtt caatgaaggt attgggctgt
     7381 tacccaagtg agaacgtagt gcctgttgat ccaacctgat gaaaaggtgc cggatgatgt
     7441 gaatcatccg gcactggatt attactggcg gttgtcattc gcctgacgca ataacacgcg
     7501 gctttcactc tgaaaacgct gtgcgtaatc gccgaaccag tgctccacct tgcggaaact
     7561 gtcaataaac gcctgcttat cgccctgctc cagcaactca atcgcctcgc cgaaacgctt
     7621 atagtaacgt ttgattaacg ccagattacg ctctgacgac ataataatgt cggcataaag
     7681 ctgcggatcc tgagcaaaca gtcgcccgac catcgccagc tcaaggcggt aaatcggcga
     7741 agagagcgcc agaagttgct caagctgaac attttcttct gccagatgca gcccataagc
     7801 aaaagtagca aagtggcgca gagcctgaat aaacgccata ttctgatcgt gctcgacagc
     7861 gctaatacga tgcagccgag cgccccagac ctgaatttgc tccagaaacc attggtatgc
     7921 ttccggctta cgtccatcac accagaccac aacttgcttt gccaggctac cgctgtccgg
     7981 gccgaacatc gggtgtaacc ccagtaccgg gccatcgtgc gccgccagca tggcctgtaa
     8041 tggtccattt ttcactgatg ccagatcaac cagaatacaa tctttcggta aaggcggtaa
     8101 tttgccgata acttgctcag taacgtggat tggcacacta acaatcacca ttccggcatc
     8161 ggcaacaata tcagccgctc gatcccagtc atgttgctcc agaatccgca cctgataacc
     8221 cgatagtgtc agcatcttct cgaacaggcg tcccatctga ccgccaccgc cgacgataac
     8281 caccggacgc agtgacggac aaagtgtttt aaatcctttg tcgttttcac tggagtaaga
     8341 ttcacgcatc acccgacgca aaacatcctc aatcagatct ggcggtacac ccagagcttc
     8401 cgcctctgca cgacgcgagg ccaacataga tgcctcgcgc tccggaacat aaataggcag
     8461 tccaaagcgg cttttcacct cgcccacttc agcaaccagt tccagacgct tcgctaataa
     8521 attcagcagc gctttatcga cttcatcaat ttgatcgcgt aatgcggtca attcagcaac
     8581 cataataaac ctcttaagcc acgcgagccg tcagctgccc gttcagatcc tgatgaattt
     8641 cacgcagcaa ggcatcggtc atttcccagc taatgcaggc atcggttacg gatacaccgt
     8701 atttcatttc actgcgcggt tgctcagaag actgattgcc ctcgtggata ttactttcga
     8761 tcatcagacc aataattgag cgattgccat ctttgatttg agcaaccacg gattctgcca
     8821 ccgcaggctg acggcgataa tctttattgg aattaccgtg gctgcaatct accatcagag
     8881 acgggcgcag tcccgcctgt tccatctctt tttcacattg cgcaacatcc gcagggctat
     8941 agttcggcgc tttaccaccg cgcaggatga catgcccgtc cggattcccc tgagtttgta
     9001 gcaacgcaac ctgccctgcc tggttaatgc caacaaaacg gtgcggctgg gcggcggcgc
     9061 gcatagcgtt aattgctgtt gccagactgc cgtcggtgcc gtttttaaaa ccaaccggca
     9121 tggaaagccc ggaggccatt tcacggtgag tttgcgattc cgttgtacga gcaccaattg
     9181 ctgaccagct aaacagatcg cccaggtatt gcgggctatt cggatctaac gcttccgtcg
     9241 ccagtggcag tcccatattc accagctcaa gcagcaattt acgcgcgatc tgcagcccgg
     9301 cttctacatc aaaagagcca tccatatggg gatcgttaat taaccctttc cagccgacag
     9361 tggtacgggg tttttcaaaa tagacgcgca ttaccagata gaggctatcg ctgacctctg
     9421 cggcaagggc tttaaatcga cgagcatatt ccagagcagt ttccggatca tgaatggaac
     9481 aaggaccaca tactaccagc agacgaggat cgcgcccggc gataatatct gaaatggttc
     9541 tacgcgagtc agcaatctgg gcttcttgtt gcaggctcaa tggaaaagcg gccttcagtt
     9601 gttccggagt cattaaaacc tgttcgtcgg taatatgtac gttattcagc gcgtcttttt
     9661 gcatgatggc gatcctgttt atgctcgttt gcgatagttg atcctcagcg aggatgacgt
     9721 aacgataaca cataaagtaa agttttcaat ccatatttcg tacattttta tttacacagg
     9781 caatttagtc gcgatttcaa cccttacttc tgtatagata aatttacact ccctttgaaa
     9841 acaatccgct atgctttgaa aaaggagaaa gaaatgatga aaaagtttat cgcccccttg
     9901 ttggctttac tggttagcgg atgtcagatt gatccttata ctcacgcgcc aaccttgacc
     9961 agcaccgact ggtatgatgt tggtatggaa gatgcgatat cgggcagcgc cataaaagat
    10021 gacgatgcat ttagcgattc acaggcggat cgcggtctat accttaaagg atatgccgaa
    10081 ggacaaaaga aaacttgcca gaccgatttt acttatgccc ggggactttc cggtaaaagc
    10141 tttcctgcca gctgcaacaa tgttgaaaat gccagccaac tgcatgaagt atggcaaaaa
//
LOCUS       DAZGLJ010000162        10195 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000162 DAZGLJ010000000
VERSION     DAZGLJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.162"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            167..319
                     /gene="hokA"
                     /locus_tag="ACHK49_003578"
     CDS             167..319
                     /gene="hokA"
                     /locus_tag="ACHK49_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO9264161.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(308..367)
                     /locus_tag="ACHK49_003579"
     CDS             complement(308..367)
                     /locus_tag="ACHK49_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264162.1"
                     /translation="MRNAVRKAGITSRRRLLLF"
     gene            complement(507..719)
                     /gene="cspA"
                     /locus_tag="ACHK49_003580"
     CDS             complement(507..719)
                     /gene="cspA"
                     /locus_tag="ACHK49_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO9264163.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1000..1290)
                     /gene="yiaG"
                     /locus_tag="ACHK49_003581"
     CDS             complement(1000..1290)
                     /gene="yiaG"
                     /locus_tag="ACHK49_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO9264164.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1724..2434
                     /gene="yiaF"
                     /locus_tag="ACHK49_003582"
     CDS             1724..2434
                     /gene="yiaF"
                     /locus_tag="ACHK49_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO9264165.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2484..3458)
                     /gene="ghrB"
                     /locus_tag="ACHK49_003583"
     CDS             complement(2484..3458)
                     /gene="ghrB"
                     /locus_tag="ACHK49_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO9264166.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3562..4221)
                     /gene="yiaD"
                     /locus_tag="ACHK49_003584"
     CDS             complement(3562..4221)
                     /gene="yiaD"
                     /locus_tag="ACHK49_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO9264167.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4374..6707
                     /gene="bisC"
                     /locus_tag="ACHK49_003585"
     CDS             4374..6707
                     /gene="bisC"
                     /locus_tag="ACHK49_003585"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO9264168.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSHGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6676..7116)
                     /gene="yiaC"
                     /locus_tag="ACHK49_003586"
     CDS             complement(6676..7116)
                     /gene="yiaC"
                     /locus_tag="ACHK49_003586"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO9264169.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7113..7676)
                     /gene="tag"
                     /locus_tag="ACHK49_003587"
     CDS             complement(7113..7676)
                     /gene="tag"
                     /locus_tag="ACHK49_003587"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO9264170.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7834..8532
                     /gene="yhjY"
                     /locus_tag="ACHK49_003588"
     CDS             7834..8532
                     /gene="yhjY"
                     /locus_tag="ACHK49_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO9264171.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8761..9963
                     /gene="yhjX"
                     /locus_tag="ACHK49_003589"
     CDS             8761..9963
                     /gene="yhjX"
                     /locus_tag="ACHK49_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9264172.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         2453 a         2458 c         2611 g         2673 t
ORIGIN      
        1 taagctagaa tagccacggg tgcttgagac tgtttgtctc aggtattcac cgaaaggcag
       61 acagagaaaa gccccacctg actataaatc aaagtgaggc taccctatgc ctgaacacca
      121 taaggttagc ctcttactcg ttggaaatca acacaggggg ctgggaatgc cgcagaaata
      181 tagattactt tctttaatag tgatttgttt cacgctttta tttttcacct ggatgataag
      241 agattcactg tgtgaattgc atattaaaca ggggagttat gagctggcgg catttttagc
      301 ctgcaattta aaagagtaag agtcttcggc gggaagttat tcccgccttt cttacggcgt
      361 tgcgcattct cattgcaccc aaatttattt ttcacaaaaa taatactaga ttttattacg
      421 cgatcgatta tttatttcct gaaaaacaaa tagaaaaatc cccgccaaat ggcagggatc
      481 ttagattctg tgcttttaag cagagattac aggctggtta cgttaccagc tgccgggcct
      541 ttagcgccgc tttcgatggt gaaggacact ttctgacctt cgtccagaga tttgtaacca
      601 tcgttctgga tagcagagaa gtgtacgaac acgtctttag agccatcgtc aggagtgatg
      661 aagccgaagc ctttgtcagc gttgaaccat tttacgatac cagtcatttt accggacata
      721 gtgtattacc tttaataatt aagtgtgcct ttcggcgata tggcgtgctt tacagatttt
      781 gaagcgttaa aggaatgtgc actacgaggg gtatcaacga taactcttga agggacttgc
      841 cttactacac tgctttaatg gtctgtacgt caaaccgttg atgtgcatta agccacgcat
      901 tggcgggtga tgcaacaatt atttttcata tttatgatta atcggttgga aaacggtcct
      961 gtcatcagga ccgtaaacag caataaagtg gataaaagtc tattccatca actgcttact
     1021 taatgccggg ttggcttgaa tcaaacgcat caattttagt tcggcacttg aaggcttcac
     1081 gcgtctggat tcccattcct ttaccatggc gactgatacg cccaaaaccc gggcgaaatc
     1141 atcgattttt aaccctgtcc cttttcgtaa ctgctcaatt tcggtacagg acgttgttct
     1201 gtgcgtcagg gtaattttct gcgtttcatc tttaaaaaca atctgttcca ggctgctcaa
     1261 cagctcatgc attggatctt tatattccat tgaaaactcc tcaaatcaca ctgcggggtc
     1321 gtgaattgca tcgaagctca ttaagaatag tcgggaaaag cagacacagc ggggtgaagt
     1381 cagggcatgc tctggctatg attcgattca gccagtcatt tagcgtgact tatagccacc
     1441 taacgatctg ataaatgccg atatatctct taccactcct ggtttgtaaa gatttcgaaa
     1501 atctaacctt ctgcgaaggc gtaaatctct ggctacaaaa aaagcaacgg cactatcatt
     1561 ttttaacaat gaaacaacaa attaaccttg tttcattttc tccatgattt taccaggaag
     1621 attacgtaga aagggtatcc tgcaagcctg tcctggacta tccttgtcac gtcagacacg
     1681 cgtgtgtcgt tgtgcgcttt ttttgggtga aaggagtaag aaaatggcga caggaaagtc
     1741 ctgctctcgc tggtttgcgc ctcttgcggc gttattaatg gtagttagcc tgagtgggtg
     1801 ttttgataaa gaaggcgatc agcgtaaagc gtttatcgac ttcctgcaga atacagtgat
     1861 gcgtagcggt gaacgtctac caaccctgac tgccgatcag aaaaaacagt ttggtccttt
     1921 tgtctctgat tacgcgattc tgtatggtta ttctcagcag gtgaatcagg cgatggattc
     1981 cggtctgcgc ccggtcgtag acagcgttaa cgcaattcgc gtaccgcagg attacgttac
     2041 gcaaagcggt ccgctgcgtg aaatgaacgg ttcgctgggt gtgctggcgc aacaactgca
     2101 aaatgcgaag ttgcaggccg atgctgcgca ctctgcgcta aaacagaccg atgatttgaa
     2161 accggtcttt gatcaggcat tcaccaaagt ggtaacgacg ccagctgatg cattgcaacc
     2221 gttgattccg gctgcacaaa ctttcacgca acaactggtg atggttggag actacattgc
     2281 tcagcaaggt actcaggtga gctttgtcgc aaatggcatc cagttcccga cttcacagca
     2341 ggcaagtgaa tataacaaac tgattgcgcc attaccggca caacatcagg cgtttaatca
     2401 ggcctggact acagcagtta ctgcaaccca ataaagagta aaagcccgag cgattatcgg
     2461 gctttacttc acgcagtcgc ggcttagtcc gcgacgtgcg gattcacaca gttcttctca
     2521 acctttcctt gtaacgcatc aatcaaatta tccacggcac aggcggccat gccataacgc
     2581 gtctcatggg tggcagatcc aatatgcggt actgcgacga cgttggccat tgagagcaac
     2641 ggcgaatcta cggacagtgg ctcttgttcg aagacatcca gcccggcggc gtgaatttcg
     2701 cctttctgca atgcagcaat cagtgcattt tcatcaacca ccgggccacg tccggcatta
     2761 atgaaaatgg cggaggattt cattttggcg aattgttctg cgccaaacag atgatgcgtc
     2821 tcatcagtta acggcaggat caggcaaacg aaatctgact cttgtaacag agtatccaaa
     2881 tcgcagtagc gggcgttgaa gcgttcttct gcttctttat ggtggcggcg cgcgttatag
     2941 aggatgggca tgttgaagcc aaagtgcgca cgttgtgcca gcgccatacc gatccgtccc
     3001 atcccgacaa tgcccagtgt tttatggtga acgtcagtgc cgtaccagtc cgggcctatg
     3061 ctcgcggtcc attcgcctgc ttttacccgt tctgctacct ccacaacccg acgagcggta
     3121 gacaacacca gcgccatcag cgtatcggcg acggtttctg ttaatacggt tggcgtgtgc
     3181 atcagcagaa tttttcgggc ggtaagcgca tcgacatcaa aattgtcata gccgacggag
     3241 atcgttgatg tggcacgcag tttcggcatt ttttccagca atgcggcatc aacattctcg
     3301 tttgaaccca gtaaaccttc agcttcggca aaaattgctg cattttgttc gacggtttgt
     3361 gggctgaggt ttgccacctg gtgaacggtg aaatgctctt gcaggcgttg cagtaaatca
     3421 tcaggtaagg ctttgtagag gataacggac ggcttcatgc ttctctccat tcactgataa
     3481 ggcgatcacc ttagcctgaa atatgatgaa ggaaaactga agaatgccag gtgatttctg
     3541 catcacctgg catgaaaggg attacagcgg gcttaaggta atttctacac ggcggttttg
     3601 cgccttacct tctgcggtgc tgttgctggc gattgggtta gccgggccaa ggccctgagt
     3661 acggatgcgg ctggcgtcca cgccctgggt gatcaacgcg ctggcaacgg aatccgcacg
     3721 ttgctgggag agacgcatgt tcaggtcgtg accacccgtg ctgtcggtat aaccaatcac
     3781 gttaaccgcc gttttcggat actctttcag caccattgcc acgccggtca gggtgttagc
     3841 gcccgcaggt ttcagggtcg cgctgctgct gtcgaaggtc acattgttcg gcatattgag
     3901 gataatgtta tccccgctgc gggttacgct aacaccagtg ccgcgcattt tgtcacgcag
     3961 cttcgcttcc tgcacatcca tgtaataacc cacgccgccg cccagagctg cgcctgctgc
     4021 tgcgccaatc agcgcgcctt taccgcgatc tttcttcgaa gaagagagcg caccaatacc
     4081 cgcgcccacg agagagccca gacctgcgcc gatagcagat ttacctgctt cgcgttcgcc
     4141 ggtgtaaggg ttagttgtgc agccagatac cgccagagcg ccactcacta cggcggcaat
     4201 aagataaaca cgtttcttca ttgttaatcc ttaataacct ttttattctt tgccacgggt
     4261 tccgtggcgg gagattatgc cgcgtgaaca tgaagattat tcctgggaat actcggaaat
     4321 ttgtaagtaa tatttaactg ctcaatacat ctaacctttc aggagttttc ggtttggcca
     4381 actcatcctc acgatattcc gttctgactg ccgcccattg ggggcccatg ctggttgaaa
     4441 ccgacggcga aaccgtgttt agctcgcatg gcgcgttagc cacaggaatg gaaaactcct
     4501 tgcagagcgc ggttcgcgac caggttcaca gcaatacacg ggtacgattt ccaatggtgc
     4561 gcaaaggctt tcttgcgtca ccggaaaatc cgcaaggcat tcgtgggcag gatgaatttg
     4621 ttcgcgtgag ttgggatgag gcgctggatc ttattcacca acaacataaa cgcattcgtg
     4681 aggcttatgg tccggcatcg atttttgctg gttcctacgg ctggcgttca aacggcgtgc
     4741 tgcataaggc ctcgacatta ttacaacgct atatggcgct ggcaggcggt tataccgggc
     4801 atctggggga ttattcgacc ggcgcggcac aggcgatcat gccgtatgtc gtgggtggta
     4861 gcgaagttta tcaacagcaa accagttggc cgctggtgct ggaacatagc gatgtcgtgg
     4921 tgttgtggag tgctaaccca ctcaatacgc tgaaaattgc gtggaatgca tctgatgagc
     4981 aggggctttc ttacttttcc gcactgcgtg acagcgggaa aaagctgatc tgtattgatc
     5041 caatgcgatc ggaaaccgtc gatttctttg gcgataaaat ggagtgggtg gcaccgcaca
     5101 tgggcaccga tgttgcgctg atgctgggga tcgcccatac gctggtggaa aatggttggc
     5161 acgacgaagc gtttctgacg cgttgcacca caggttatgc cgtcttcgcc tcttatttgc
     5221 tgggcgagag tgacggaata gcgaaaaacg ccgaatgggc ggcagagatt tgtggggtta
     5281 acgcagcgaa aatccgcgag ctggcggctc ttttccacca aaacacgacc atgctgatgg
     5341 ctggctgggg aatgcagcgc caacagtttg gtgagcaaaa acactggatg atcgtcacgc
     5401 tggcggcaat gttggggcaa atcggcacac ccggcggcgg ttttggtctt tcttatcatt
     5461 ttgccaatgg tggtaacccc acgcggcgtt ctgcggtgct ctcttccatg cagggcagct
     5521 tgccgggtgg ctgcgatgcg gtggataaaa tccctgttgc ccgcattgtt gaagcactgg
     5581 aaaaccctgg tggcgcatat caacacaacg gtatgaaccg acatttcccg gatattcgtt
     5641 ttatctggtg ggcgggcggt gccaacttta ctcatcatca ggataccaat cgtctgatcc
     5701 gtgcctggca aaaaccggag ctggtggtga tctctgaatg cttctggacg gcggcggcga
     5761 aacacgcgga tatcgttctg cctgcgacta cctcttttga gcgtaatgat ctcaccatga
     5821 ccggtgatta cagtaatcag catctggtgc cgatgaagca agtggtgccg ccacgctatg
     5881 aagcgcgtaa tgattttgat gtttttgccg agttgagtga acgttgggag aagggcggct
     5941 atgcgcggtt tacggaagga aaaagtcagc tgcaatggct ggaaacgttt tataacgttg
     6001 cccggcagcg cggggcaagc cagcaggttg aattgccgcc atttgctgag ttctggcaag
     6061 ccaaccagtt aattgagatg ccggaaaacc cggacagcga gcggtttatt cgcttcgctg
     6121 atttttgccg cgatccgctg gcgcatccgt taaaaaccgc cagcggcaag attgaaatct
     6181 tctcacagcg tattgccgat tacggttacc cggattgccc tgggcatcca atgtggctgg
     6241 agccggacga atggcagggc aatgccgaac cagaacagtt gcaggtactt tctgcccatc
     6301 cggcgcaccg cctgcacagc cagctgaatt acagttctct gcgcgaattg tacgcggtag
     6361 caaaccgtga gcctgtcacc attcatcctg acgatgctca ggcgcgcggc ataaccgaag
     6421 gggatatggt gcgggtgtgg aactcccgtg ggcagatcct tgccggagcg gtcattagcg
     6481 agggaattaa acctggcgtg atttgcattc acgaaggggc atggccggat ctggatttaa
     6541 ccgctgacgg tatttgtaaa aacggcgcag tgaacgtgct gaccaaagat ctccccagct
     6601 cgcggctggg gaatggctgt gcgggtaata cggcgctggc atggctggaa aaatacaacg
     6661 gtccggaact gacacttaca gcgtttgaac caccggccag ctcataatcc aggtgggtag
     6721 ttgggtttca tcctgccatg cgcaatcgac aatgtgaaaa ccctgcgcac ggtaaaaatc
     6781 tatcgccggt tgattttttt gataaacctc cagcatcaga tggggatagc gctgctgcac
     6841 atactgcatc agcgccttac caataccgcg tctgacggcc ttcggtgcga caaacattgc
     6901 tgccagaaat cggccttcca taatgctgac aaaaccgaga agcttaccgt cctcttccca
     6961 gacccagttt tgcgcgttgg caagataggc atcccgcacc agcggaatgc agtcacgcca
     7021 gtaattcgct tttataaagg gatgccccca ggttgtactt tccagccaca gttcgaggat
     7081 cgcggggagt tctgaacgtt gcgcttcccg aatcatggtt tatttcccgg atagcaacag
     7141 cagccaacca catgatcatt caccagccca catgcctgca taaaggagta acagattgtg
     7201 gtgccgacaa acttaaaacc acgttttttc agtgccttag atagggcgtc ggaggcggac
     7261 gtagatgtgg gaatttcgct caacgttgtg gcttgtgtca cctgtggctg atgatttaca
     7321 aacgaccaga caaagtcggc aaacggttcg ccgttctgtt ccatttgcag gtacgcccgc
     7381 gcattaccaa taattgcctg aattttccct cgatggcgga taatcccggc gtcctgtacc
     7441 agtctttcga catcctcttc ctgcattgct gcgaccttca ccggatcgaa ctgatgaaag
     7501 caggcgcgat agttttcgcg ttttttgagg acggtgatcc acgataaccc agcctgctgc
     7561 ccttcaaggc agatcatttc gaacagtttt ttactgtcag tttcaggcac gccccattca
     7621 ttatcatggt aggcaatata aagcgggtcc tgactcaccc agccgcaacg ttccatactt
     7681 tccctcgcta tgtacccgat cgtaaaaatt tcagccgctt ttcttgacgc atcggtgaaa
     7741 aagtcaatat ttaatacagg gctactatgc ccgatatcct tcatgcaatg acaataatat
     7801 tgccgaattc ggctcttccc tggagtcgtg ttaatgatca taaaaaaaag cggtggtcgc
     7861 tggcagctaa gcctgctggc gagcgtggta atcagtgcct tttttctcaa cacagcttac
     7921 gcctggcaac aagaatatat cgttgatacg caacccggac attccacaga gcgttacacc
     7981 tgggatagtg atcatcaacc tgattacaac gatattttgt cgcaacgtat tcaaagtagc
     8041 caaagggcgc tgggactgga agtcaatctg gcggaagaaa ctcctgtgga tgtgaccagc
     8101 agtatgagta tgggctggaa ttttccttta tatgaacagg ttacaaccgg cccggtcgcg
     8161 gcattacatt acgatggcac aaccacctcg atgtataacg agtttggcga cagtactacc
     8221 acgctgaccg atccgttatg gcatgccagc gtgagtagct taggctggcg tgttgactcc
     8281 cggcttggcg atctccgacc ctgggcgcaa atcagctata accagcaatt tggcgagaat
     8341 atctggaagg cgcaatcagg cctgagccgg atgacggcga caaaccagaa cggcaactgg
     8401 ctggatgtca ccgtaggcgc tgatatgttg ctcaatcaaa atattgccgc ctatgccgcg
     8461 ctaactcagg cagaaaatac cactaataat agcgactatc tgtatacgat gggggttagc
     8521 gccagatttt aacgtaacag tcacaattga aaccattgaa taacaacagt tgtggcgata
     8581 gtgggtgcta acttaccaaa taataaattt ggtgaataat tgtcgcgtca ttcattcctg
     8641 aactaaggca tttcattccg ttctgatggc atttcatgcc gtttttcccc aggcataaaa
     8701 tgcacttcgt tatggttgtc ggcagagatt tttccttttt attactgcag gaatactgcc
     8761 atgacacctt caaattatca gcgtacccgc tggctgacac tcatcggtac tatcattacc
     8821 cagtttgcac tggggtcggt ttatacctgg agcctgttta atggcgcgct ttctgccaag
     8881 ctggatgcgc cggtaagcca ggtcgctttc tctttcggct tgttaagtct ggggctggca
     8941 atttcgtctt ctgttgcggg caaattgcag gaacgttttg gcgttaaacg cgtcaccatg
     9001 gcttccggca ttttgctggg attaggcttc ttcctgaccg cgcattccaa caacctgatg
     9061 atgctgtggt taagcgccgg tgtgctggtc ggtctggcgg atggcgcggg ttacctgctg
     9121 acgctctcta actgcgtgaa gtggttcccg gagcgtaaag gtctgatatc cgcgttcgct
     9181 atcggttctt atggtctggg tagcctgggt ttcaaattta tcgacacgca gctgctggaa
     9241 acggtgggtc tggaaaaaac ctttgtgatt tggggagcga ttgcgctggt gatgattgtt
     9301 ttcggcgcaa cgttaatgaa agacgcacca aaacaggaag tgaaaaccag caatggtgtg
     9361 gtggagaaag attacacgct ggcagagtcg atgcgtaaac cgcagtactg gatgttagcg
     9421 gtaatgttcc tgaccgcctg catgagcggc ctgtacgtga ttggggtagc gaaagatatc
     9481 gcccaaagtc tggcacacct tgatgtggtt tccgcagcca atgcagtcac tgttatttcc
     9541 atcgccaacc tttcaggtcg tctggtgctg ggtattctgt ctgacaaaat cgcccgtatc
     9601 cgtgttatca ccattggtca ggtgatatcg ctggtgggta tggcggccct gctgtttgca
     9661 ccattgaatg cagtgacgtt ctttgcagcg attgcctgcg tggcatttaa ctttggcggc
     9721 actattaccg tctttccgtc actggtcagt gagttctttg gcctcaataa cctggcgaaa
     9781 aactacggtg tgatttacct cggtttcggt atcggtagca tttgtggttc gattatcgcc
     9841 tcactgtttg gcggcttcta tgtgaccttc tacgtgattt tcgccctgct gattctgtca
     9901 ttggcgcttt ctacgacaat tcgtcagcca gagcagaaag tattgcgtga ggcgcatatt
     9961 tagaaatatg ttgctactga aataaatgcg aggagggaga cttcaaagtc tccctttttt
    10021 tatggacgca tattaaaaga acaataatat ttacaccaca aatatatttt atttgtttat
    10081 atatcatgta aatataaata tattcctaaa aaaatagtcg ttggaagcgt ttttgtgtgc
    10141 aattaattga agttgaagta catttcaatt tattgcaaaa actaatcttc agtaa
//
LOCUS       DAZGLJ010000163        10192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000163 DAZGLJ010000000
VERSION     DAZGLJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.163"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            154..1089
                     /locus_tag="ACHK49_003590"
     CDS             154..1089
                     /locus_tag="ACHK49_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO9264173.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELVAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLARSRLGIMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(1145..1687)
                     /locus_tag="ACHK49_003591"
     CDS             complement(1145..1687)
                     /locus_tag="ACHK49_003591"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9264174.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1671..1982)
                     /locus_tag="ACHK49_003592"
     CDS             complement(1671..1982)
                     /locus_tag="ACHK49_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO9264175.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(2167..3057)
                     /gene="cyoE"
                     /locus_tag="ACHK49_003593"
     CDS             complement(2167..3057)
                     /gene="cyoE"
                     /locus_tag="ACHK49_003593"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO9264176.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(3069..3398)
                     /locus_tag="ACHK49_003594"
     CDS             complement(3069..3398)
                     /locus_tag="ACHK49_003594"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO9264177.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(3398..4012)
                     /locus_tag="ACHK49_003595"
     CDS             complement(3398..4012)
                     /locus_tag="ACHK49_003595"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO9264178.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(4002..5993)
                     /gene="cyoB"
                     /locus_tag="ACHK49_003596"
     CDS             complement(4002..5993)
                     /gene="cyoB"
                     /locus_tag="ACHK49_003596"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO9264179.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(6015..6962)
                     /gene="cyoA"
                     /locus_tag="ACHK49_003597"
     CDS             complement(6015..6962)
                     /gene="cyoA"
                     /locus_tag="ACHK49_003597"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO9264180.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(7422..8897)
                     /gene="ampG"
                     /locus_tag="ACHK49_003598"
     CDS             complement(7422..8897)
                     /gene="ampG"
                     /locus_tag="ACHK49_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO9264181.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTGVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(8941..9519)
                     /locus_tag="ACHK49_003599"
     CDS             complement(8941..9519)
                     /locus_tag="ACHK49_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO9264182.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            9824..10141
                     /gene="bolA"
                     /locus_tag="ACHK49_003600"
     CDS             9824..10141
                     /gene="bolA"
                     /locus_tag="ACHK49_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO9264183.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
BASE COUNT         2804 a         2509 c         2551 g         2328 t
ORIGIN      
        1 ctaaataaac gatttggtga catggatgat aatgtttcag gctaaatgtg tgcttattac
       61 ttggaccttt atggcactgt atttattcat taaatggata tttggtggtt aattattatg
      121 gaaatatatg acagtgagga cataaaggta tacatgttaa ctaaaagaat attattttct
      181 gtaatgctga tagtttcacc aagcgtggtg gccagcgaga aagcgcatga attgtatgac
      241 agcatatacg gtggtaaacc tgctcccgat gttataaata cactgcataa aatggctgaa
      301 tcgggagata tcgacgcgca gagcctgtta ggctgggaat attatcagcc tcgttatgat
      361 accaaacctg atgttcagga agcgattaaa tggttcgagt tagtggctaa acaaggtgac
      421 agagaagctc cgttagcgct ggggggtatc tactacgacg gtgagcaggt gcgggtggat
      481 tatgccaaag cgtatgcact atttaatcag gccgcgcagc acggtgtgaa tttagcaagg
      541 tccaggttgg gcattatgta cgccaatggt cagtatgttg aggtagattg taagaaagcg
      601 aaggaatatc ttgataaagg tgtccacatt tatggtggcc cagaagactt tctggctact
      661 tgtcgaaaag acatgattga cagaaaaacg gttgacgata cgttacccgt gattacggtt
      721 acccgttctg ggatgcgaga taatttttta gataaaggtt tttcctgcat ggatagcctc
      781 ttcgccacca ccaataaatt aggcgaagtg gccaatcttc gcgtgacatt cagcattcgt
      841 cgcccatccg gtaaggagat taaccaaacg gttggcttcg cgcctttcgg tttaaaccgg
      901 ctgaatatta gctttactga ttacctattt ggttcattta ccagtaattc gtctcttatt
      961 ctttataaac cggaatttga gcgaaaatct tgtgcaactg taaggaccac aatcgttgcc
     1021 gcaacggcaa ccattaacgg caaggatgtg gagttgctga aagcgggggc aattgaacaa
     1081 aagtggtaac gctgctcagc ccccggctga ggcaaaactt gccctggcgg gtggcgataa
     1141 taatctaagc cgcgagaata tgctgaatcg ccaaaaacat aggtacagca ccaaaagcat
     1201 ttggtgtcgc tgaaagctga acaaagccga gacgagcata aaaattaatg gcggcagggt
     1261 cagcatcaag ataaacccct ttaattggta atgcctggtg aattattttt acatgttcaa
     1321 aaaaatcaca tagtaggtcc tgaccaaagc cccgcttttg atacttccgt gctaccccca
     1381 acatgactaa tctgacaaca ccaatttctg aaggttgtga accctgaagg acgccagaaa
     1441 cgcgttgttt ttccagcgaa tatgccgtaa aagtacagat gccgatcagt tcaccacttt
     1501 gtctgtcaat aagtgcttta gccgcacagt cgctattacg cacacttttc tttagcgatg
     1561 cgcgtacaaa tttatcgata acagggttac cgcaatcaaa ctgtttttgc ccaggataag
     1621 taatatcaga ctgataatta caggagagga ctatgggcag agtaatctct tcatgcttgt
     1681 cgaccatcgc tgtttttcct tttcattaac gccttgaggg cgagcgtcgg ttgagcaggt
     1741 tcagcgatga gttggttcat cagttcccag ctttgattcg aaagctcacg gcgacgttgg
     1801 ttatcaagca cactttcggc gcgttccata gcagcgctca aaataaaagc gcttaaatct
     1861 accccaacgg cttgcgcagc ttcacgaagt ttgtttttgg cgtcgggact agttttgagt
     1921 tcgacccgcg catttttcag cgttttatcg acatcgggta gagtggctgt ttttccaggc
     1981 attatgacct ctttgattaa ctgaatgaaa tcgtagggag tcgatctcat acataatacg
     2041 gctatcaacc gtatgattaa tttatgttca tctgctcact ctgtcaagac atacggttat
     2101 tagccgtatc tctgatctca gacaataaaa aaggtgccgt agcacctttt taatagagag
     2161 gttttgttac cacacagcag ccagcagcgt atgcgagtcc ggtaccataa aatcaacgga
     2221 catcatcacc gagagggcag tgatggcgat gatagagaag ccgaacagct tgcgcgccca
     2281 gattctgtca tcagcaactt tataaccgcg cagagccata cctaaccacc agacgctaac
     2341 cgccgcggcg accaccagat atttatatcc agcgtaaccg ccaagagaga gcatcagcgt
     2401 ggcaacggca aaggcgatga tatacagcgt gatgtgattc ttcgccaccg aaatgccttt
     2461 taccactggc aataccggaa tgtttgccgc ctggtaatcc ttaaagcgga aaatggcgat
     2521 ggcataggag tgaggcatct gccacaggct gaagatagcc agcaggatcg ctgcgccgct
     2581 atcgaactcg ccggttactg cacagtagcc aatcaccggc ggcgcagcgc cggagagcga
     2641 accaatcaat gtgccgtaga ctgagtggcg tttcatgtac aggctataga cgccgacata
     2701 aaccacaaag cccatcaccc ccagccagca ggccagcgga ttcgcgccaa accacagcag
     2761 cataaagcca gcaataccca gcaaggtggc gtacaccagc gagacagcag gagagatcag
     2821 gcctttaacc agcacccgat tcttcgtcct ttccatcttt ctgtcgatat ccctgtcgat
     2881 gtagttgtta aacacacaac ccgacgccac aaccagtgac accccaacca gcgtgtagat
     2941 aaacagggga taatcaatgc tgccctttga ggccagcagg aatcccccaa tcaccgagat
     3001 caggttgcca aagatgatgc ctggtttcgt tacttgcagg tattgcttaa acatcataac
     3061 cgccgctctt agtgcatcat catgttgtag ttgaggttcc acataatcca gatggagcct
     3121 acaaccagga tagcgatgat tagcacggtg aagacaaacg ccgtcatgtt ccagccttca
     3181 tctgatttgg tattcatgtg caggaagcac accagatgca ccagaacctg taccactgcc
     3241 attgccagga ttgttcccag aattacggcc ggagaggcag cccctgtcat caccatccag
     3301 aacggaatca ccgtcagaat gatcgacagg ataaagcctg tcatgtaggt ttttacgctg
     3361 ccatgggacg cgccgctgtg atcggtagaa tgactcatta catcgccccc atcagataaa
     3421 caacagtgaa cacacagatc caaaccacat ccaggaagtg ccagaacagg ctcaggcaca
     3481 tgatgcgggt acggttagtg ctggtcaggc cgcgacgagc gatttgtacc atcagcaccg
     3541 ccatccagat aagaccggaa gtgacgtgca gaccgtgcgt gccgaccagc gcaaagaacg
     3601 ctgacaggaa gccgctgcga tccggaccca tgccgttaac aatcaggtga tggaattcgt
     3661 agatttccat cccgataaat ccggcaccaa acaaccaggt caacgccagc caggagataa
     3721 cctggctttt gttgtttttg tacatggcga tagccgccat gccgtaggtg atggagctga
     3781 acaacagcaa gaaagtttca accagaacga acggcagttc gaaaatgtcc ttacctgtcg
     3841 ggccgcctgc ggtgccgttc accagaacgg cataggtagc aaacaagatt gagaacagaa
     3901 tgcagtcgct catcaggtag atccaaaatc cgaagatttt ggttccgcct gcatcgtggt
     3961 gcccgtgttc gtgcgcgtgg gcagtcgcgt gcgtcaaagt atcagttgcc atttttcagc
     4021 cctgccttag taatctcatc gaaatgctgg ttttccagtt tttcgatttc tgccaccggc
     4081 acgtagtaat ccacgtcctc gtcgaagctt ttcacgatcc aggtgatgat catgcctgcg
     4141 aagccaacaa tcgccagcca ccagatatgc cagatcatgg cgaaaccgaa gatggtggag
     4201 aaagctgcaa tgacgatacc tgcaccgctg tttttcggca tatgaatttc ttcatagtgg
     4261 tcaggctttt tatacgcttc gcctttctct ttcatttccc agaatgcatc acgctcgtga
     4321 acgtgcggca caacggcaaa gttatagaac ggaggcgggg aagaggttgc ccactccagc
     4381 gtacggccac cccacgggtc gccagtcagg tcacggttct ggtcgcggtc gcgaatagaa
     4441 acgtacatct gaataacgag gcagagaata cccagcgcaa tcagtactgc accgctagct
     4501 gcaatcatca gcatggtgtg gaactgcggg tcaatctgct ggctcaaacg acgggtcatg
     4561 cccatgaagc ccagcgcata cagcggcata aaggcaacga agaagccgat gatccagaac
     4621 cagaacgcgc gtttacccca ggtttcattc agtttgaaac cgaaggcttt aggccaccag
     4681 taggtcatcc ctgcgaagca gccgaagacc acgccgccga tgatcacgtt atggaagtgc
     4741 gcaatcagga acaggctgtt atgcagaacg aagtccgcgc ccggtactgc cagcagcacg
     4801 ccagtcatcc cgcccaccga gaaagtgacg ataaaaccga tggtccacag catcgcagaa
     4861 tggaacacga tgcggccctg atacatggtg aacagccagt tgaagatctt caccccggtc
     4921 gggatggcga taatcattgt ggtgatacca aagaaggcgt ttacgttcgc gcccgcaccc
     4981 atcgtaaaga agtggtgcag ccaaacgatg aacgacagca cggtgataca gacggttgcc
     5041 cataccagcg aggtataacc aaacagacgt ttacgcgaga aggttgccgc aatttcggag
     5101 aacacaccga aaacaggcag gatcaggatg taaacttccg ggtggcccca ggcccaaatc
     5161 aggttgatgt acatcatcat gttgccaccc atatcgttgg taaagaaatg ggtgcccaga
     5221 tagcgatcca gggtcaacaa cgcgacggta accgtcagaa ttgggaagga agcaataatc
     5281 agtacgttcg cgcacagtga tgcccaggta aatactggca tcttgaacat ggtcatgccc
     5341 ggtgcgcgca tcttcagaat ggtaacgaag aagttgatac cggtaagcgt cgtacctata
     5401 ccggatagct ggagactcca tatccagtaa tcgacaccga ctcccggact gtactctatt
     5461 cccgatagcg gtggataggc cagccagccg gtctgcgcaa attcgcccac gccgagagaa
     5521 acgttaacca gaatcacacc aacaacagta aaccagaagc ttaagttgtt gaggaacggg
     5581 aacgcaacgt cacgcgcgcc gatctgcagc ggaaccacca ggttcatcag accgataacg
     5641 aaaggcatcg ccacgaagaa gatcataatc acgccgtgcg cggtgaagat ctgatcgtag
     5701 tggtgaggtg gcaggaagcc cgcttcgccc gccgaggcaa gagcctgctg gctacgcatc
     5761 ataatggcgt cagcaaaacc acgcagcaac atcacaatcg ccacgatgat atacatgata
     5821 ccgaggcgtt tatggtcgac ggaggtcagc cactctttcc acaggtaggt ccacttaccg
     5881 aagtaagtga tcaggccaac gagcgccaga cctcccaaaa taatgccagc gatcgtaacc
     5941 atgacgatag gttcatggaa cgggactgca tcaagtgata attttccgaa catctttatt
     6001 cttcctcaac ccctttaatg ggcggattcc gcgtggctca tgtccatgcc ttccatacct
     6061 tcgtgtgcac tgtgctcacc ttctggctgg gtcatgtcca tgctcttacc gtgagccata
     6121 aacttgttaa tcacatcggc aaacaagtct ggtttcacgt tggagaaata ttccacctgg
     6181 ttgtattcgc taggcgcggc cagtttttcg aacgcagcca tgtcagacat ggtgttcggc
     6241 gactgtttcg cttttgcgac ccattggtcg aattcggcgc gatccggtgt tgcaatagct
     6301 ttgaacttca tgcctgagaa gcccggcccg ctgtagctgg cggagatacc gtcataagta
     6361 ccgggttcgt tggcgatcag atgcaggcga gtctgcatac cggccatggc ataaatctgg
     6421 ctacccagac gcggaatgaa gaaggagttc atcacggagt tggaggtcac tttgaagtac
     6481 accggagtgt tcgccgggaa agcgatttca ttcacggtag caatgccctg ttccgggtag
     6541 atgaagaacc atttccagtc catggaaacc acttcgatgg taatgggctt ctcgtcgtgt
     6601 gccagcggct tgctaggctc aagagcgtga gtggttttcc aggtcagtac cgcaaggaag
     6661 atgatgatta agataggtac cgtccagacc acagcttcca ctttattgga gtgtgaccag
     6721 ttcgggctgt acttagcatc tttattgctc gcacgatact tccaggcgaa accaacagcc
     6781 atcaagattg cgggaataac gacaatcaac atcaggccaa atgccgtcag tatcagtgaa
     6841 cgttgctcca gaccaatctg tcctttggga tctaacagcg cagaattaca gccactgagc
     6901 aatacagtgc ctgcaaataa tgacaaccat cccaaacttt tattgtattt cctgagtctc
     6961 atttaacgac ctcaattcca cgggatctgg tggcgtttaa agtgtgtggg cattttacgg
     7021 gaaggttaca ttactgtaaa catgattaaa ttcctgttac ttggatttgg ctgtcaggtc
     7081 acatatgttg caaaacatat caagttgatt ttaagaagct gttgcaaaag ggcgttataa
     7141 cgaaagggga gtgcagaagg agtttggacg ggcaattggt ataaccaatc tggaataaaa
     7201 caattgttta acaaataatt atcattatgg tgacaattgg gtgggggaaa ataaagactc
     7261 attagctgaa tcgtgtaaga aaaaatagaa ttcataaatt gcgtcaataa tttccaaacg
     7321 ctaagccgca caaaagaaca aatattatta agcgatactg atgtttattt agccgtaata
     7381 attacggcga gtgattacta cagctaaata atatttacag attacgtcag atgcgttttt
     7441 cgtagcgcca gataatccag caaaccacca agcacgacgc cagaaagggc gactaaaacc
     7501 ccgacttcca gcagagcagg caggaaagag aagtgcgtca aatccagcgc gtccatcgtc
     7561 aacagcagta accacacggc caacaggctg acgcccgccg ccagtgtcca catggcaaag
     7621 gcataacctg ccggatattc ggtacgggag ataaagttgt catttactcc tgtatattca
     7681 agcgtctggc ggcaaaccag cagcaaaata agccccggta cggcagcggc gacggagaat
     7741 agatagaatg tcgaccagcc gtgtgcttca acaaaccaac ccgccacggg gccgacataa
     7801 actcgtccta cagcggaaag cgctgagagc agggcaaatt gagttgcgga aaatgactta
     7861 ttacacagcg tcattaacag cgcgacaaag gctgatgtgc ccatcccgcc acagaggttt
     7921 tcgaaaaaga cggctgcgcc catgctgtag agatgcttat cagtaatcga cagcagccag
     7981 taaccagcgt tagacgcacc ttgtaaaatg ccgaaaatca gcagcgcccg gaacagcgac
     8041 aggcgctgca tcaaaatccc accgtacaat gcgcccacaa tggtcgccag taagccaagc
     8101 gttttgttaa ccacgcctac ttcacccgca tcaaacccga cgccgcgaat caaaaacgtg
     8161 gttgtcaggc tcatggcgaa tgcgtcgccc agcttataca gcacgataag aagcaaaata
     8221 agccaggcat tattgcgacc aaagaaatct cgcagaggtg caacaaccgc ttgttccagc
     8281 gtttttggca caggaatggt gtcggtcggt tctggtgcaa gcaacgtcgc gataatacaa
     8341 gggatcaaca gcgccgccat caaccagtac atgccctgcc agcccagcca tttatctgcc
     8401 agccacaggg ctaagccgcc ggaaaccagc atccctaaac ggtaacccag cacgctgatt
     8461 gccgcgcccg caccacgttc ttctgccggg agcacatcgg ttttccacgc atcgaagaca
     8521 atatcctggg aggccgagca aaaagcgatc accaccgcca gtgccgccat ccagcggagt
     8581 tgggtgccag gttcgagaaa acccatcgcc gcaatggcga ctaataacag gatttgcgtg
     8641 gcgagcagcc aaccgcgccg ccgcccaaaa aatgggggcg tgtagcggtc catcagcggt
     8701 gaccagagga atttaaaaac gtaagcctgg cctaccagag agaagaaacc aatggttttg
     8761 agatcaatat tctcgaccgt catccaggcc tgtaaggtgc cggatgtcag ggcgagcggt
     8821 agcccggaag caaaacccag gatcagcaat atggctgaac gcggctgttg aaaaatacgt
     8881 aaatattgac tggacatggg cgtctacagg cccggcttgc accgggccag agggcagaaa
     8941 ttaacgcgcg ttctgcttga tgaattcgtg aatgctggtg tcctgagaca tatcagcgat
     9001 ggtatcggtc agcacactgt taaccgcatc ggcgatattt ttgttggagg cctggaacgc
     9061 accttcaaca ttgtagctgg cacgatagtt tttggtcatt ttgttgccat tctgcgcggt
     9121 agcgatgatg gcgatatccg ctttggtagc gatgttgtag cgcacgttgc cctgggacac
     9181 gtcagcatac agttggctaa cgatgatttg cagattaacc gggccattcg gaccaaccat
     9241 gtaaccacgc gcggtcatct gtttttccag cacttcttgc agcaggaaac gcagatcgcg
     9301 ggaggcggtc agggtaacga tttgattatc gcgggtgact tttgccagcg cctgatcggt
     9361 acgctgatcg gcaccattaa tgcttacggt gacgcccatc aggcttggat cctgctgtgg
     9421 cagtgtaatc gtcggggaaa cttcaatagt tgttggcggt tttgcgcatc ctgcaagcat
     9481 aaacagagca actaacggga agaggatttt tttgaacatg ttcgggctct cagagactct
     9541 taagcgtgtt tggtaaaaat tcccgccatc ataacattgc caacggcgag gggaagtggg
     9601 taaggcatgt aaattcatca tgttgacgaa ataatcaccc ctggtaaaag aaacgctgat
     9661 gcgaggcctg tgtttcaatc tttaaatcag taaacttcat acgcttgacg gaaaaaccag
     9721 gacgaaacct aaatatttgt tgttaagctg caatggaaac ggtaaaagcg gctagtattt
     9781 aaagggatgg atgacatctc agcgttgtcg gaggagatat ttcatgatga tacgtgagcg
     9841 gatagaagaa aaattaaggg cggcgttcca acccgtattc ctcgaagtag tggatgaaag
     9901 ctatcgtcac aatgtcccag ccggctctga aagccatttt aaagttgtgc tggtcagcga
     9961 tcgttttacg ggtgaacgtt ttctgaatcg tcatcgaatg atttacagta ctttagcgga
    10021 ggaactctct actaccgttc atgcgctggc tctgcatact tacactatta aggagtggga
    10081 agggttgcag gacaccgtct ttgcctctcc tccctgtcgt ggagcaggaa gcatcgcgta
    10141 aaaacgcatt tgcaactgtc ggcgcttttc cagtatgttg ctaaagattt ta
//
LOCUS       DAZGLJ010000164        10119 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000164 DAZGLJ010000000
VERSION     DAZGLJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.164"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1102
                     /locus_tag="ACHK49_003601"
     CDS             <1..1102
                     /locus_tag="ACHK49_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO9264184.1"
                     /translation="ARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTLAVSIAM
                     VARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVILVGIKMM
                     ILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADKVKDPQV
                     IMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHVGYVIVV
                     IGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVVLIMLMT
                     AALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVIVGFIYS
                     LWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            1133..1230
                     /locus_tag="ACHK49_003602"
                     /pseudo
     CDS             1133..1230
                     /locus_tag="ACHK49_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1227..1634
                     /gene="cueR"
                     /locus_tag="ACHK49_003603"
     CDS             1227..1634
                     /gene="cueR"
                     /locus_tag="ACHK49_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO9264185.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(1635..2093)
                     /gene="ybbJ"
                     /locus_tag="ACHK49_003604"
     CDS             complement(1635..2093)
                     /gene="ybbJ"
                     /locus_tag="ACHK49_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO9264186.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(2090..3007)
                     /gene="qmcA"
                     /locus_tag="ACHK49_003605"
     CDS             complement(2090..3007)
                     /gene="qmcA"
                     /locus_tag="ACHK49_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO9264187.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            3153..3830
                     /gene="fetA"
                     /locus_tag="ACHK49_003606"
     CDS             3153..3830
                     /gene="fetA"
                     /locus_tag="ACHK49_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO9264188.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTNGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            3817..4596
                     /gene="fetB"
                     /locus_tag="ACHK49_003607"
     CDS             3817..4596
                     /gene="fetB"
                     /locus_tag="ACHK49_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO9264189.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(4659..5513)
                     /gene="cnoX"
                     /locus_tag="ACHK49_003608"
     CDS             complement(4659..5513)
                     /gene="cnoX"
                     /locus_tag="ACHK49_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO9264190.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAETVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(5574..6383)
                     /gene="ybbO"
                     /locus_tag="ACHK49_003609"
     CDS             complement(5574..6383)
                     /gene="ybbO"
                     /locus_tag="ACHK49_003609"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO9264191.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(6373..6996)
                     /gene="tesA"
                     /locus_tag="ACHK49_003610"
     CDS             complement(6373..6996)
                     /gene="tesA"
                     /locus_tag="ACHK49_003610"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO9264192.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            6967..7653
                     /gene="ybbA"
                     /locus_tag="ACHK49_003611"
     CDS             6967..7653
                     /gene="ybbA"
                     /locus_tag="ACHK49_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO9264193.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            7650..10064
                     /gene="ybbP"
                     /locus_tag="ACHK49_003612"
     CDS             7650..10064
                     /gene="ybbP"
                     /locus_tag="ACHK49_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO9264194.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVEPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2225 a         2614 c         2827 g         2453 t
ORIGIN      
        1 tgcgcgtctg ggggcgagct atcccagcaa tggcggcatt atcgacttct ttcgtcgcgg
       61 attaggcaac ggcgtctttt cgctggcgct ctcgttactg tacctgttga cgctggcggt
      121 gagcatcgcc atggtcgccc gtgcttttgg cgcttatgcc gtgcagtttt tgcatgaagg
      181 cagccaggag gagcacctta ttttgctcta cgcgttgggg atcattgcgg tgatgacgct
      241 tttcaactcc ttaagcaacc atgcggtagg gcggctggaa gtgatcctcg tcggcattaa
      301 aatgatgatc ctgttattgc tgattattgc cggtgtctgg tcgctgcaac cggcgcatat
      361 ttccgtctct gcgcccccca gctccggtgc gttcttctcc tgtatcggga taaccttcct
      421 tgcctatgcg ggctttggca tgatggcgaa cgcggcggat aaagtgaaag atccgcaggt
      481 cattatgccg cgggcgtttc tggtggcgat tggcgttacc acgttgcttt atatctcgct
      541 ggcactggtt ttgcttagcg atgtatcggc attagagtta gaaaaatatg ccgataccgc
      601 cgtagcgcag gctgcttctc cgctgctcgg acatgtgggt tatgtgatcg tcgtcatcgg
      661 cgctttactg gcgacggctt cagccattaa cgcgaacctg ttcgccgtgt ttaacatcat
      721 ggacaacatg ggcagcgaac gcgaactgcc gaagctaatg aataaatccc tgtggcggca
      781 gagtacctgg ggcaacatca ttgtcgtggt gttgattatg ctgatgacgg cggcactaaa
      841 tttaggctca ctcgccagcg ttgccagcgc cacctttttg atttgctatc tggcggtgtt
      901 tgtggtggcg atccgcctgc gtcatgatat tcacgcctcg ttgccgattc ttatcgttgg
      961 tacgttggtg atgttgttgg tgatcgttgg ctttatctac agtctgtggt cccagggtag
     1021 ccgtgcgttg atatggatta ttggctcact cttactcagc cttattgtgg caatggtcat
     1081 gaagcgcaat aaaaccgtat aacatctctc tgtgcgcagt acttcctgta ttattgtggt
     1141 ggcggtcgat attcgcactg gcaaaaaaac gtgcttgaat atctgttgaa accctttaac
     1201 aaagcacagg aggcgttgcg cgaacgatga acatcagcga tgtagcaaaa attaccggcc
     1261 tgaccagcaa agccattcgc ttctatgaag agaaggggct ggtgacgccg ccgatgcgca
     1321 gcgaaaacgg ttatcgcacc tacacgcagc agcatctcaa cgaactgacc ttactgcgcc
     1381 aggcacggca ggtgggcttt aacctggaag agagcggcga gctggtgaat ctgtttaacg
     1441 acccgcagcg gcacagcgcc gacgtcaaac ggcgcacgct ggagaaggtg gcggagatcg
     1501 aacgacacat tgaggagctg caatccatgc gcgaccagct gctggcactg gcgaatgcct
     1561 gccctggcga tgacagcgcc gactgcccga ttatcgaaaa tctctccggc tgctgtcatc
     1621 atcgggcagg gtgattaaga cgagacggct cggatatgta gggttatccc ttctatcgca
     1681 atgacttcaa catgcgtacc tgcaccgaga tcctcgctgg cgctgacagg ccatgaactg
     1741 tcaccgacgc gcatatgacc gcgcccgttg accagcggag attccagcac aaaacgtcgg
     1801 ccaatcagct gctgcccgcg ctggtttaaa tgactatcgc tgtgcttttg ttcgcgcacc
     1861 cgccgcgaca accatttcca ccacagccag gcggcgagca gcgtcaggat ggcaaacatt
     1921 accccttgcc actcccaacc cagcggcacc agccagacca ccaggccggt aatcaccgct
     1981 gccacgccgc tccacaacaa ataaccattt ccgcccagca tctcggctgc cagcagcaaa
     2041 ccgccgagac tgagccagaa aatatgtgga tgaacgacca ttaactccat catggctgag
     2101 tccgtttgtt ggcgctgtct ttcaccagct cggcaatccc ggcaatcgac cccatcaggc
     2161 tgctggcctc taatggcatc atcactactt tgctgttact ggaggaaccg atctgctgta
     2221 acgcttcggt gtatttctgc gctacgaagt agttcaccgc ctgaatatca ccggaggcga
     2281 tagcttcaga caccattttg gtggcgcggg cttctgcttc ggcggaacgt tcacgcgctt
     2341 cagcctgtaa aaacgccgac tgacgttcgc cttccgcttt caggatttgc gactgttttt
     2401 caccttcggc tttgaggatt tccgcctgac ggatcccttc cgcttcaaga atgtaagcgc
     2461 gtttggtacg ttccgctttc atctgcgcgt tcattgaaga gataagctct gccggtgggc
     2521 gcacgtcgcg aatttcaata cgggtgactt taatccccca cgggttggtg gcttcatcga
     2581 caatacgcag caggcgtgag ttgatgctgt cgcgctgaga gagcatttcg tcaagttcca
     2641 ttgaacccaa cacggtacgg atgttagtca tggtcaggtt gatgatcgcc agctccagat
     2701 tgctgacttc ataagccgcg cgcggcgcgt caatcacctg aataaaacag actgcgtcga
     2761 tggtaacgtt ggcgttatct ttcgagataa cttcctggga agggatatcg agcacttgct
     2821 ccatcatatt gatcttgcga ccaatgcgat ccataaacgg caccaccaga ctgagccccg
     2881 gctgtaacgt tttggtatag cgaccaaaac gttctaccgt ccattgatag ccctgcggca
     2941 cgattttgac gcccgcgccg acaatgacca gcgcgacaaa aatgagaatc gggataaaga
     3001 taagcatcgg aaaaacctcc tgttgtaccg tccataatca gcaaaattgc tgcttgatta
     3061 aacaaattat acctgattac tgaaagagag ttccccctta ttcctgcgaa ggataaactg
     3121 tttttagtaa aaatcagaaa aagggaacag tgatgcagga aaatagtcct ttacttcagc
     3181 tacaaaacgt aggatatctg gcgggtgatg cgaagattct taataacatc aatttttcgc
     3241 tgcgtgctgg cgaatttaag ttaattaccg gtccttccgg ttgtggcaaa agtacgctgc
     3301 taaaaatagt tgcttcattg atcagcccaa ccaacggaac gttactgttt gaaggtgagg
     3361 atgtcagcac actaaagcca gaaatctacc gccagcaagt ctcttactgc gcccagacgc
     3421 cgacgctgtt tggcgatacg gtatacgata atctgatctt tccctggcag atccgtaacc
     3481 agcagcctga cccggccatt tttctcgatt ttctcgaacg cttcgctttg ccggacagca
     3541 ttttgacgaa aaatatcgcc gagctatctg gtggtgaaaa acaacgcatc tcactgattc
     3601 gtaacctgca atttatgccg aaggttttat tgctggatga aatcaccagt gcgctggatg
     3661 aaagtaataa acataacgtt aatgagatga tccatcgtta tgtgcgcgag caaaatattg
     3721 ccgtgctgtg ggtgacacac gataaagacg aaattaatca tgcggataaa gtgattacac
     3781 tgcaaccgca tgccggagaa atgcaggaag cacgctatga actcgcataa tattactaac
     3841 gaatcattgg cactggcatt aatgctggtg gtggtggcaa tcttaattag ccataaagaa
     3901 aaactggcgc tggagaaaga tattttctgg agcgtcgggc gagcgataat tcagctgatt
     3961 attgtcggct atgtgctgaa gtatattttc agcgtggatg atgccagcct gacattattg
     4021 atggtgttat ttatctgctt taatgcggcg tggaacgcgc aaaaacgcag taaatatatt
     4081 gctaaagctt ttatctcatc gtttattgcc attacggtcg gggcgggaat taccctggcg
     4141 gtgctgattc tctctgggtc gattgaattt atcccgatgc aggtgatccc tatcgccggg
     4201 atgattgccg gtaacgccat ggtagcggtg gggttgtgtt acaacaattt agggcaacgg
     4261 gtcattagcg aacagcaaca gattcaggag aaactgagtc ttggtgcgac gccgaagcag
     4321 gcttcagcga tattgattcg cgacagtatt cgcgcggctt taattccgac ggtcgattca
     4381 gcaaaaacgg ttggcttagt gagtttacct ggaatgatgt ccgggctgat atttgccggg
     4441 attgatccgg tgaaggcgat taaatatcag attatggtga cctttatgct gctctcaacc
     4501 gccagcttgt cgaccattat tgcctgctat ttaacctatc gtaagtttta taattcgcgc
     4561 caccagttgg tggtgacgca attgaagaag aaatgatgcg atgccggatg cgcagcatcg
     4621 catccggcgt tgtggttcat gtgccgatca acggaatatc aatacaacaa tgcatacagc
     4681 tggcggcgat acttcgacgc cagtgcatca cccgtaccca gcgcagcgag gatctcctga
     4741 aacgttttac gcgtctgacc gtctgcggcg gtgagatctt tacgcagatg cccgaacagc
     4801 aactccagcg cctcttcatt gcgcccaacc tggtgcagtt gcagagccag ttgcgtcgcc
     4861 agtgcggcat cttctggatt ctccgccacc tgctgttgca actgttgaat ttccggcgta
     4921 tcagccgcct gcttcagcag ttcgatttgc gccaccagcc cctggtagcg ggtgtcctga
     4981 tcctgcaacg gaatggtttt cagcaccgtt tccgcatctt cagaacggtt cagcgcaatc
     5041 agcgtttctg ccagcagcag gccgatctcc ccgttctgat tcgacaactg ccaggcgtct
     5101 ttcagcaatg gcagggcatc ggtgtaattg ccttcctgca tcagttgcat cgcctgctgc
     5161 gctttcagct cttcttcgcg cggcagcact ttatccagca gggcgcggat cgcctcttcc
     5221 ggttgcggcc cctggaagcc atctaccggt tgcccgttct ggaacagata cacggtcgga
     5281 atcgcgcgca gaccaaactg ggcggcaatc atctgctccg cgtcgcagtc cagcttcgcc
     5341 agaataaatt gcccgttgta ctgcgccgcg aggctttcca ggatcggggt taactgcaaa
     5401 cagtgctggc tacgttcaga ccaaaaatag aacagcaccg gagtggtcat cgactgttca
     5461 agaacctgtt gcaggttaga ttcgttaatg ttgacaatat tttctacgga catggagtcg
     5521 ctctctgttg tcgatatttt ctttgacatg ggggcttaag cgcgcgcttc aactcacccc
     5581 tgcaatattt tgtccatcac gcgccccggc agcaggcgct tgagcaccat tactgcccag
     5641 gtcaccagcg ttaccggata gcgcatcttc ggcttctcgc taataaaagc atggcgtact
     5701 ttgtccacca ccgcttccgg tcccaacgta aagcgggcgg cgatgccggg attttcgact
     5761 ggtttatcac tttgcgtctg gttgacgttg tcggtgaagc gagtacgaat gggaccgggt
     5821 tcgatcaggc tgactttaat tccgctgtgg cgcagctcca tgcgcagtgc gtctgaccac
     5881 gcctccagcg catatttact ggccgcgtaa gcgccacgac ccggcgtgga gattaatccc
     5941 atcaccgatg atgtcatcac aatacgccct tcaccgtgcg gtaacatcgc gggtaacagg
     6001 cgcatggtga gctggtgtgc gccgaaaaag ttggcggaaa actgctgttc catctgcgca
     6061 cggctgatgg tggaaagggg gccatacatg ccgaatccgg cattgttaaa gatcccatac
     6121 agacaattat cggtcagggc gatcacctcg tctgctgcgc gatcaacact ttctggtgaa
     6181 tccagatcga tcaacacgcc ggtaaatccc atgttgttca tacgctcaac atcatccggt
     6241 tttcggcaac ctgccagcac atgaaaaccc tggcgtttta attcgagcgc gctttccagg
     6301 ccaattccac tggaacatcc ggtaattaag accgattttt gcataacttt acctgtcagg
     6361 atctccgttg ctttatgagt catgatttac taaaggctgc aactgcttcg ccatccagtc
     6421 ggcaataaac ggctgggcgt cgcggttggg atgaataccg tcatcctgca tccattgtgg
     6481 cttgaggtag acctcttcca taaaaaaggg cagcagcgga acatcaaact ctttggcgag
     6541 tttggggtaa atggcgctaa aggcttcatt ataacggcga ccatagtttg caggcagacg
     6601 tatttgcatt aacaatggtt cagcgttggc ggctttgaca tcctgcaaaa tctggcgcag
     6661 cgtttgctcg gtttgctgtg gctgaaaacc acgcaaaccg tcattgccgc ccagttcaac
     6721 cagcacccag cgcggctgat gctgtttcag cagagccgga aggcgcgcca gtccttgttg
     6781 cgaggtgtcg ccgctgatgc tggcattaac taccgacgtt ttactctgcc acttatcatt
     6841 caacaaggca ggccaggccg cgctggcaga cattcgatac ccggcgctca ggctatcacc
     6901 cagaatcaat aacgtgtccg ctgcggcggc acggaaggtt aacaggacca ggaacaggaa
     6961 gggcaaatgc cagcggaaaa cattgttgaa gttcatcatc ttaagaagtc cgtcggtcag
     7021 ggggagcatg aactctccat cctcaccgga gttgagctgg ttgtcaaacg tggcgagacc
     7081 atcgcactgg tgggcgaatc gggatcgggt aagtcaacct tgctggcgat cctcgccggg
     7141 cttgatgacg gcagcagtgg cgaagtgagt ctggtgggac aaccgctaca taatatggac
     7201 gaagaagcgc gggcaaagtt gcgcgcgaag cacgtcggct ttgtttttca gtcatttatg
     7261 ttaattccta cccttaacgc gctggaaaat gtcgagcttc cggctctgct gcgcggtgag
     7321 agtagcgcgg aaagtcgtaa cggggcgaaa gcgttgctcg aacagttagg gctgggtaaa
     7381 cgtctggatc atcttccggc acagctttcc ggcggtgaac agcaacgagt ggcgctggca
     7441 cgagccttta atggtcgacc tgatgtgctg tttgccgacg aacccaccgg caaccttgac
     7501 cgccagacgg gcgataaaat tgccgacctg ctgttttccc tcaaccgtga acatggcacc
     7561 acgttgatta tggtgactca cgacctgcaa ctggcggcac gctgcgaccg ctgcttacgg
     7621 ctggtgaacg ggcagttgca ggaggaagca tgattgcacg ttggttctgg cgcgaatggc
     7681 gctcgccgtc gctattaatt gtctggctgg cgctaagcct ggcggtggcc tgcgtgctgg
     7741 cgctgggcaa tatcagcgat cgcatggaga agggcttaag ccagcaaagc cgtgagttta
     7801 tggcgggcga tcgggcgttg cgcagttcgc gcgaagtgcc gcaagcatgg ctggaggaag
     7861 cgcaaaagcg cggcctgaaa gtcggcaagc agctgacttt cgccaccatg acatttgcag
     7921 gcgacacacc gcagctggcg aacgtcaaag cggtggatga tatctacccg atgtatggcg
     7981 atctgcaaac taatccccct ggcctgaaac cgcaggcggg cagcgtattg ctggccccac
     8041 gcctgatggc actgcttaac ctgaaaacgg gcgacaccat tgacgtgggc gatgccacct
     8101 tgcggattgc cggagaagtg attcaggaac cggattccgg ttttaacccc ttccagatgg
     8161 ctccgcgtct gatgatgaat ctggcggatg tcgataaaac cggagccgtg cagccgggga
     8221 gtcgggtcac ctggcgttat aaattcggcg gcaacgagaa ccagctcgac ggctatgaga
     8281 aatggttgtt acctcagctt aaacccgaac aacgctggta cggtctggaa caggacgaag
     8341 gcgcgctggg gcgatcgatg gaacgctcgc aacagttcct gctgctttcg gcgcttctga
     8401 ccttgctgct ggcagtggca gcggtggcgg tagcgatgaa tcattactgt cgcagtcgct
     8461 acgatctggt ggcgatcctc aaaacgctgg gggcagggcg agcgcaactg cgtaagctaa
     8521 tcgtcggtca gtggttgatg gtgctggcgc tttcagccgt taccggtggg gcgataggcc
     8581 tgttgttcga aaacgtgttg atggtgctgc tcaagcccgt tctgcctgct gcactaccgc
     8641 cagccagcct ctggccgtgg ctgtgggcgc tgggtaccat gacggtcatc tcgctgctgg
     8701 tggggctacg accgtaccgc ctgttgctgg caacgcagcc tttacgcgta ttacgtaatg
     8761 atgtggtagc gaacgtctgg ccgctgaagt tttatctgcc gattgtcagt gtggtggttg
     8821 tgctgctgct cgccggatta atgggtggca gcatgctgct ttgggcggtg ctggcgggcg
     8881 cggtagtact ggctttgctg tgcggtgtgc tgggctggat gctgctgaat gtacttcgcc
     8941 gcatgacgct gaaatcgcta cctctgcgcc tggcggttag ccgcctgtta cgtcagccgt
     9001 ggtcaacatt aagccagctt tcggcatttt cgctctcctt tatgctgctg gcgctgttgc
     9061 tggtattgcg tggcgatctg cttgaccgct ggcagcagca gctacctcca gaaagcccga
     9121 actacttttt aattaacatt gccacagaac aggtagaacc gctaaaagcg ttcctcgcgg
     9181 aacatcagat agtcccggaa tcattttatc cggtagtgcg ggcgcggctg acggcgatta
     9241 acgataagcc gacagaaggt aatgaagatg aggcgcttaa ccgcgaactc aatcttacct
     9301 ggcaaaatac gcgacccgat cataatccga ttgtcgccgg taactggccg ccaaaagccg
     9361 atgaagtgtc gatggaagag gggctggcga aacgcttaaa cgttgccctc ggtgataccg
     9421 tgacttttat gggcgatacc caggagttcc gcgctaaagt gaccagcctg cgcaaagtgg
     9481 actgggaaag tctgcggcct aatttctatt ttattttccc tgaaggggca ttagacgggc
     9541 aaccgcagag ctggcttacc agtttccgct gggagaatgg caacggcatg ttgacacaac
     9601 tcaaccgcca gttcccgacc attagcctgt tagatattgg cgcgatttta aaacaggtcg
     9661 gtcaggtgct ggagcaggta agtcgggcgc tggaggtgat ggtggtgctg gtcaccgcct
     9721 gcggtatgtt gctgttgctg gcacaggtgc aggtgggaat gcgccagcgt catcaggagc
     9781 tggtggtatg gcgcacactc ggtgcgggga aaaaactgct gcgcacgaca ttgtggtgtg
     9841 agttcgccat gcttgggttt gtttccggcc tggtggccgc aattggtgcg gaaacggcac
     9901 tggcagtgtt gcaggcgaaa gtgtttgatt tcccgtggga gccagactgg cgattgtgga
     9961 ttgttctgcc gtgcagcggt gcgctgctgc tgtcgctttt cggcggctgg ctgggtgcgc
    10021 gactggttaa gggtaaggcg ctgttcaggc agtttgcggg gtgatgagag taaataaggg
    10081 gcgttctggt tgaatggaac gccttgttta aattaattc
//
LOCUS       DAZGLJ010000165        10059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000165 DAZGLJ010000000
VERSION     DAZGLJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.165"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            102..1280
                     /gene="purT"
                     /locus_tag="ACHK49_003613"
     CDS             102..1280
                     /gene="purT"
                     /locus_tag="ACHK49_003613"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO9264195.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEQLQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGLQIRLFGKPAINGSRRLGVALATAESVVDAIKRAKHAAGQVKVQG
                     "
     gene            complement(1336..1977)
                     /gene="kdgA"
                     /locus_tag="ACHK49_003614"
                     /gene_synonym="eda"
     CDS             complement(1336..1977)
                     /gene="kdgA"
                     /locus_tag="ACHK49_003614"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO9264196.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(2014..3825)
                     /gene="edd"
                     /locus_tag="ACHK49_003615"
     CDS             complement(2014..3825)
                     /gene="edd"
                     /locus_tag="ACHK49_003615"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO9264197.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(4060..5535)
                     /gene="zwf"
                     /locus_tag="ACHK49_003616"
     CDS             complement(4060..5535)
                     /gene="zwf"
                     /locus_tag="ACHK49_003616"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO9264198.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            5873..6742
                     /gene="hexR"
                     /locus_tag="ACHK49_003617"
                     /gene_synonym="yebK"
     CDS             5873..6742
                     /gene="hexR"
                     /locus_tag="ACHK49_003617"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO9264199.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            6870..8312
                     /gene="pyk"
                     /locus_tag="ACHK49_003618"
     CDS             6870..8312
                     /gene="pyk"
                     /locus_tag="ACHK49_003618"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO9264200.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(8372..9214)
                     /locus_tag="ACHK49_003619"
     CDS             complement(8372..9214)
                     /locus_tag="ACHK49_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFO9264201.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASIDVLRKAVFEGANIIITHEPTFYSYADLEGDVLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDDMYHVYAIEP
                     DSAINITKRFAKNLKIYNLPGIQFYGDRARIVRTIGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGYSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
     gene            complement(9237..>10059)
                     /locus_tag="ACHK49_003620"
     CDS             complement(9237..>10059)
                     /locus_tag="ACHK49_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9264202.1"
                     /translation="YGNRYHVLQSFPMPQLASSREIRILLPASYYESDVSYPVLYMHD
                     GQNLFDKSVSFGPHVWDIPEAVDAFFPNSQYDGVIIVGIDNASSHGKFSRMDEYSPWP
                     LNTSFPLPGWDPDVDYSGGKGALYVDFIVNTLKNYIDSNFRTLPDRNNTAIAGSSMGA
                     YISLFAAILRLDVFSKVGVFSPALWFNDSAMLNFIQENNIFADLTVYLDVGTLETSGM
                     REDFPEVYISGAEKLCVSLRKQRNVTIDYHLWGGDTHSESAWAKRFPEMLKLFYC"
BASE COUNT         2576 a         2563 c         2406 g         2514 t
ORIGIN      
        1 cagtatattt cttttcggtt gagaaatcaa catcagcaat aaagacacac gcaaacgttt
       61 tcgtttatac tgcgcgcgga attaatcagg ggatattcgt tatgacgtta ttaggcactg
      121 cgctgcgtcc ggcagcaact cgcgtgatgt tattaggctc cggtgaactg ggtaaagaag
      181 tggcaatcga gtgtcagcgt ctcggcgtag aggtgattgc cgtcgatcgc tatgccgacg
      241 caccagccat gcatgtcgcg catcgctccc atgtcattaa tatgcttgat ggtgatgcat
      301 tacgccgtgt ggttgaactg gaaaaaccac attatatcgt gccggagatc gaagctattg
      361 ccaccgatat gctgatccaa cttgaagagg aaggactgaa tgttgtcccc tgcgctcgcg
      421 caacgaaatt aacgatgaat cgcgagggta tccgtcgcct ggcggcagaa cagctgcagc
      481 tgcccacttc cacttatcgt tttgccgata gcgaaagcct tttccgcgag gcggttgccg
      541 ccattggcta tccctgcatt gtaaaaccgg tgatgagctc ttccggcaag gggcagacgt
      601 ttattcgctc tgcagagcaa cttgctcagg catgggaata cgctcagcaa ggcggtcgcg
      661 ccggagcggg ccgcgtaatt gttgaaggcg tcgttaagtt tgacttcgaa attaccctgc
      721 taaccgtcag cgcggtggat ggcgtccatt tctgtgcacc agtaggtcat cgccaggaag
      781 atggcgacta ccgtgaatcc tggcaaccac agcaaatgag cccgcttgcc cttgaacgtg
      841 cgcaggagat tgcccgtaaa gtggtgctgg cactgggcgg ttatggtctg tttggtgtcg
      901 agctgtttgt ctgtggtgat gaggtgattt tcagtgaggt ctcccctcgt ccacatgata
      961 ccgggatggt gacgttaatt tctcaagatc tctcagagtt tgccctgcat gtacgtgcct
     1021 tcctcggact tccggttggc gggatccgcc agtatggtcc tgcagcttct gccgttatcc
     1081 tgccacaact gaccagtcag aatgtcacgt ttgataatgt gcagaatgcc gtaggcgcag
     1141 gtttgcagat tcgtttattt ggtaagccgg caattaatgg cagccgtcgt ctgggggtgg
     1201 cactggctac tgcagagagt gttgttgacg ccattaaacg cgcgaagcac gccgccggac
     1261 aggtaaaagt acagggttaa acccggcaaa aaaacgctac aaaaatgccc gatccgcgat
     1321 cgggcatttt gacttttaca gcttagcgcc ttctacagct tcacgcgcca gcttagtaat
     1381 acggtcgtaa tcgcccgctt ccagcgcatc tgccggaacc agccaggaac caccgatgca
     1441 cagcacgctt ttcagcgcca ggtagtcacg gtagttagcc ggagaaatac cacctgtcgg
     1501 gcagaaacgg acctgggaga acggacccgc gatcgcctgc agggctttca cgccgccgtt
     1561 agcttcagcc gggaagaatt tgaactcttt caaaccgtag tccataccca gcatcagttc
     1621 ggaaacagtg ctgatccccg gaatcagagg aatagtccct tcggtagcag ctttcagcag
     1681 cggctcggtc agacccgggc taattgcgaa ctgtgcaccc gcttcggtga cttctgccag
     1741 ctgctgtgga ttcagcaccg taccggcacc cacaatcgct tcaggcactt ctttggcgat
     1801 agcacggata gcgtcaactg cacactcggt acgcagagtc acttccagaa cgcgcacccc
     1861 accagcaacc aacgcttttg ccatcggcac tgcgtgttcc agttttttta ccacgataac
     1921 cggtacaacc gggccggtgg tcaggattga ttctgcactt gttttccagt ttttcatcag
     1981 agttttctct cgcctgatta caaatttgtc gtcttaaaaa gtgatacagg ttgcgccctg
     2041 ttcggcaccg gacagttttt cacgcaaggc gctgaataat tcacgtcctg tccccacgcg
     2101 tgacgcgctc aggtcaggaa tgtgcggttc gcgagcagcc agttccgctt cgtctaccag
     2161 cagcgtcagt tcgcctgtct gtccattcac acgaatgatg tccccgtcgc gcacttttgc
     2221 cagcagcccg ccatcgtagg cttctggtgt tacgtggata gctgacggca ctttacctga
     2281 agcgccggag agtcgtccat cggtaactaa cgcaattttg aaacaccggt ccaataatac
     2341 accaagtggc ggcatgagtt tatgtaattc tggcattccg ttcgcttttg gcccctgatg
     2401 acggacaaca acgacacaat cgcggtccag caaacccgct tcaaaggccg gcataacgtc
     2461 atgctggctt tcaaaaacaa ccgctggcgc ttcaatcacc tggttctcaa ccggcacggc
     2521 agaggttttc ataaccgcac ggcccaggtt accgcttaac acttttgtcc caccatgatg
     2581 agagaaaggt tgttcgaagg aagcgatcac attgctgtcg agtgattttt ccgccccttc
     2641 ccgccagtcc agttcaccat tattcagcca tggttcaagg gtataacgag acagaccaaa
     2701 gcctgccacc gtattgacat cttcatgcag caggcctgct ttgagcagtt cacgcaccag
     2761 aaccggtacg ccacctgccg cctggaagtg gttaatatcg gccggaccgt tcgggtagag
     2821 acgtgccatc agcggtacaa catcagaaag gtcagagaag tcatcccagt taatctgaat
     2881 accggccgcg cgcgccatcg ccaccaggtg catggtgtgg ttagtggaac caccggtcgc
     2941 cagcagtgca acgataccgt tcaccaccac tttctcatcg atcatcttac cgatcggcat
     3001 ccattcatta ccattaccgg tcatgcgtgt aacctgacgc gcagcggcag cggtcaaagc
     3061 atcgcgcagc ggagaatccg gatgaacaaa agaagagcct ggcaactgca tccccataaa
     3121 ttccaccacc atctggttgg tgttggcagt accgtagaaa gtacatgttc ccggcgcatg
     3181 gtaagacgcg gcttctgact ccagtaaggc catgcggtcc actttacctt cggcataaag
     3241 ctggcgaata cgcacttttt ctttatttgg caaaccgctt gccatcggtc cagacggcac
     3301 aaacaccgca ggcaaatgac caaacgacag ggctgccatc gtcagacccg ggacaatctt
     3361 gtcgcacaca ccgaggaaca gagcaccatc aaacatgtta tgggacagcc cgaccgccgc
     3421 agacatcgct atcacttcgc ggcttagcag cgacaattcc attccatcct gcccctgggt
     3481 gacaccatca cacatcgccg gaacaccgcc cgcaacctga ccaaccgcat tcgcttcatg
     3541 cagggcttta cgaatgattt ctggatagtg ttcataaggc tggtgcgcgg agagcatgtc
     3601 gttataggag gtgatgatgg cgatattgtt acgcaacatg cttttcaaag aggctttgtc
     3661 ttctggctgg caggcagcga aaccgtgtgc caggttaccg catgccaact gcgaacgatg
     3721 aacggtcgaa gttttcgctt gttctatccg ggcgagataa gcagagcgag tctcgcgcga
     3781 acgttcaatg attcgatttg ttacgcgtaa caattgtgga ttcataaagg ctcctgaaat
     3841 tgagttgtca gagcaggatg attcacaacg cgtttcattc agaggattta tgactgaaac
     3901 gcctgtaacc ggagctcata gggcaaaaac gtttcagtca gtgtaataaa aaaagcctcg
     3961 tgggtgaatc cgcacgaggc ctgaaagtgt aaaaattgtt ctacaatctg tgcaagatca
     4021 tgttaccggt aaaataacca taaaggataa gcgcagatat tactcaaact cattccagga
     4081 acgaccatca cgggtaatca tcgccaccga ggcaacgggt ccccaggttc cggcctgata
     4141 cggtttcggc gcatcattgt ccatcgccca cgcctcagta atggagtcta cccatttcca
     4201 ggcttcttcc acttcgtcgc gacgaacaaa cagtgcctga ataccacgca tggtttccag
     4261 cagcaaacgt tcataggcat ccgccagatg cgtctgatta aaggtttctg aatagctcag
     4321 atccagcttg gtgatttgca ggttatgttt gtggtcaagg ccaggaactt tattcagtac
     4381 ctggatatcc acgccttcat caggttgcag acggatagtc agtttattct gcggcagatc
     4441 ctgccacgat tctttaaaca gattcagttc aggtgttttg aaatagacca cgacttcaga
     4501 acatttggtc ggcagacgtt taccagtacg caggtagaat ggcacaccgg cccagcgcca
     4561 gttatcaatg tcgacgcgga tcgctacgaa ggtttctgta ttgctgctct tgttcgcgcc
     4621 ctcttcttcc agatatcccg gcactttttt gccctgggcg aagcccgcag tatattgccc
     4681 gcgtacggtt ttttcgcgta cgttggagcg gtcgatgcgg cgcagagact tcagtacttt
     4741 cactttttca tcgcggatgc tgtctgcgct caggtcagac ggcggagaca tcgcaatcat
     4801 gcagagaatt tgcagcaggt ggttttggat catatcgcgc atctgaccgg ctttatcaaa
     4861 atagccccag cgcccttcga tccccacttc ttctgccacg gtaatctcaa catgatcaat
     4921 ggtgcgattg tcccagttat tcacaaacag ggagttagca aaacgcagcg ccaacaggtt
     4981 cagcaccgtt tctttaccaa gatagtggtc gatacggtaa acctggcact cctcgaagta
     5041 ttcgccaacc tgatcattga tttcctgcga ggtcgccagc gacgtcccca gcggtttctc
     5101 catgactacg cgtgccggtt tagcattcag tttagcctcg ccaagccctt tgcaaattgc
     5161 gccaaaagtg ctgggcggca tggcaaagta gttaatggtg atacgatttt tttgatccag
     5221 catcgcgccg agacggctga atgcagcagt gtcattgaca tcgagattac aaaaatccag
     5281 acgtgcactc agggtgtccc ataaaccttc atcaatggtt tctttcatga aagtttcgag
     5341 cgcctcgcgg acaactttgg tatatgccgc tttatcccag tcagcacgcc ctacgccgat
     5401 aatccgggtg tccgggttga gctgaccggc tttttccagt tgatacaggg aaggcagcaa
     5461 tttacgacgc gcaaggtcgc ctttcgcgcc gaaaatgacc aggtcacagg cctgggctgt
     5521 ttgcgttacc gccatgtcat tctccttaag ttaactaacc cggtacttaa gccagggtat
     5581 acttgtaatt ttcttacggt gcactgtact gcttttacga gcttgcgaaa actgtaaacg
     5641 cttatccacc cgtgcgatta cggtaaaagc gcgcaaagtg cggcaaaact gataaaaaaa
     5701 tcatcgtttt ccattgggta aaaatctgac actgatcatg ttatgaaaaa aaataacaac
     5761 ttttttatct gcttttgtca ttaacacagc acacaggcgt aatatctgac aaaactacat
     5821 ttcgatttct ttcagtgcgg aaatcgtcat tacccgtgag tttctttaca tcatgaatat
     5881 gctggaaaaa atccagtctc agctggaaca tttgagcaaa tcagagcgca aagttgccga
     5941 ggtcattctg gcttcgcccg ataacgcgat ccattcgagt attgctgctc tggcgctgga
     6001 agccaatgtt agcgaaccga cggtgaatcg tttctgtcgc agcatggaca cgcgcggttt
     6061 tcctgatttt aaacttcatc tggcacaaag tctggcgaat ggcactccct atgttaatcg
     6121 caatgtcaat gaagatgaca gcgttgaatc atacacaggg aaaatatttg agtccgcaat
     6181 ggcaacgctt gatcatgtcc gtcattcact ggataaatct gccatcaacc gcgccgtcga
     6241 cttgctcact caggcaaaaa aaatcgcctt tttcggatta ggctcttcag ccgccgttgc
     6301 ccacgatgcg atgaataagt tctttcgttt taatgttccg gtggtgtact ccgatgatat
     6361 cgtgctgcaa cgcatgagtt gtatgaattg tagcgacgga gacgtggtgg tgctgatttc
     6421 tcacactgga agaacaaaaa atctggtcga gctggcgcag ctggctcgcg aaaacgacgc
     6481 catggtgatt gccctcacct ctgcgggtac cccgctcgcc cgggaagcaa cgctggcaat
     6541 taccctcgac gtaccggaag atactgacat ttatatgccc atggtttctc gacttgcaca
     6601 gctgaccgtg atagatgtgc tggcgacagg atttactttg cgacgcggtg caaaattcag
     6661 agataacttg aagcgggtca aggaagcgct gaaggaatcg cgttttgata agcagttact
     6721 taatttaagt gacgatcgct aaaaacgact gtcactgtcc taatcttata cgacatccga
     6781 atgagattaa tttatcgcca tcgcggcgtt atttcattcg gatttcatgt tcaagcaaca
     6841 cctggttgtt tcagtcaacg gagtattaca tgtccagaag gcttcgcaga acaaaaatcg
     6901 ttaccacgtt aggcccagca acagatcgcg ataataatct tgaaaaagtt atcgcggcgg
     6961 gtgccaacgt tgtacgtatg aacttttctc acggctcgcc tgaagatcac aaaatgcgcg
     7021 cggataaagt tcgtgagatt gccgcaaaac tggggcgtca tgtggctatt ctgggtgacc
     7081 tccaggggcc caaaatccgt gtatccacct ttaaagaagg caaagttttc ctcaatattg
     7141 gggataaatt cctgctcgac gccaacctgg gtaaaggtga aggcgacaaa gaaaaagtcg
     7201 gtatcgacta caaaggcctg cctgctgacg ttgtgcctgg cgacatcctg ctgctggacg
     7261 atggtcgcgt tcagttaaaa gtactggaag ttcagggcat gaaagtgttc accgaagtga
     7321 ccgtcggtgg tcccctctcc aacaataaag gtatcaacaa acttggcggc ggtttgtcgg
     7381 ctgaagcgct gaccgaaaaa gacaaagcag acattaagac tgcggcgttg attggcgtag
     7441 attacctggc tgtctccttc ccacgctgcg gcgaagatct gaactatgcc cgtcgcctgg
     7501 cacgcgatgc aggatgtgat gcgaaaattg ttgccaaggt tgaacgtgcg gaagccgttt
     7561 gcagccagga tgcaatggat gacatcatcc tcgcctctga cgtggtaatg gttgcacgcg
     7621 gcgacctcgg tgtggaaatt ggcgacccgg aactggtcgg cattcagaaa gcgttgatcc
     7681 gtcgtgcgcg tcagcttaac cgcgcggtaa tcacggcgac ccagatgatg gagtcaatga
     7741 ttactaaccc gatgccgacg cgtgcagaag tcatggacgt agcaaacgcc gttctggatg
     7801 gtactgacgc tgtgatgctg tctgcagaaa ctgccgctgg gcagtatccg tcagaaactg
     7861 ttgcagccat ggcgcgcgtt tgcctgggtg cggaaaaaat cccgagcatc aacgtttcta
     7921 aacaccgtct ggacgttcag ttcgacaatg tggaagaagc tattgccatg tcagcaatgt
     7981 acgcagctaa ccacctgaaa ggcgttacgg cgattattac catgaccgaa tcgggtcgta
     8041 ccgcgctgat gacctcccgt atcagctctg gtctaccaat tttcgccatg tcgcgccatg
     8101 aacgtacgct gaacctgact gctctctatc gtggcgttac gccggtgcac tttgatagcg
     8161 ctaatgacgg cgtagcagct gccagcgaag cggttaatct gctacgcgat aaaggttact
     8221 tgatgtctgg tgacctggtg attgtcaccc agggcgacgt gatgagtacc gtgggttcta
     8281 ctaataccac gcgtatttta acggtagagt aatctcacct gccccggccg ctgatgaccg
     8341 gggtatttag caacacataa attaggacta ctcattcaat ccaatcataa tcacagcccc
     8401 ctgtaaaatg aatacaggga tagcctgaat ggagatctaa atagcttgca agacgtcgca
     8461 tacccgcttc ctctgcaaca ctatatccaa taactgccat cggtaagcca ctatctttgg
     8521 agtattgtgt ctgcacccat gttttaattg aatcgttaat cgtaatataa taatcagcct
     8581 gatattccat atattgtatt ggatcacaaa aacagcctgc cccaatccca atagttctaa
     8641 ctattctggc tctatctcca taaaattgga tcccaggaag attataaatt ttcaaatttt
     8701 tggcaaaacg tttggtaata ttaatggcac tgtcaggttc tatagcatat acatggtaca
     8761 tatcatcaga agcaacaata tttgtaacat ccagttctaa ctgctgagcc aaagccttag
     8821 acattccgaa cgtaggctct cgatccatga catcatggca acgaatgatt accaaattat
     8881 ttttatgcaa aaactccttt ttttgctcaa ttatttttaa ataactgagt tcaccgcgcg
     8941 tataatccat tatttttctg gcccatgaaa actcaagaac gtcaccctcc agatcagcat
     9001 aagaataaaa tgtaggctca tgagtaataa tgatattggc accttcaaaa acagcctttc
     9061 ttaaaacatc aattgaggca atccagcagg tcccaatttt attaataagt gtcgcctcag
     9121 caccaaaaat aattttatca acagttggtt gcgaaacggc aaccattgaa tttaaaaaac
     9181 aattaagatc tgcggctgtt ttaatatgat gcattcaaca tccttcaccg gtttcatcag
     9241 caataaaata atttcaacat ttctggaaaa cgttttgccc atgcagactc agagtgggta
     9301 tcacctcccc atagatgata atctatagtt acattgcgtt gtttacggag actcacgcat
     9361 aatttttccg cgcctgaaat atagacttca ggaaagtctt cgcgcatacc actggtttcc
     9421 agcgtaccga catcgagata tacagtaaga tctgcaaaga tattgttttc ctgaataaaa
     9481 ttcagcattg cgctgtcatt gaaccatagt gccggcgaaa aaacgccaac ttttgaaaaa
     9541 acgtctagac gaagaattgc ggcaaacaaa ctgatgtacg cacccataga actacctgct
     9601 atcgctgtat tattacgatc tgggagagtt cgaaaattgc tgtcaatata gttcttgagc
     9661 gtattcacaa taaaatcaac atacaacgca ccttttcctc cactataatc cacatcaggg
     9721 tcccagccag gtaagggaaa agatgtgttt agcggccatg gagaatactc atccatacgg
     9781 ctgaatttac catgagacga tgcattatca atccccacaa tgataacacc gtcgtattgg
     9841 gaatttggga aaaaagcatc taccgcctca ggtatatccc atacatgtgg accaaaagat
     9901 acagatttat caaataaatt ttgcccatcg tgcatataca atacagggta actgacatca
     9961 gattcataat aggacgcagg tagtaatatt cttatttcgc gactacttgc caattgtggc
    10021 atcggaaagg attgcaacac atgatatcga ttcccatag
//
LOCUS       DAZGLJ010000166         9960 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000166 DAZGLJ010000000
VERSION     DAZGLJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.166"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(186..261)
                     /locus_tag="ACHK49_003621"
     tRNA            complement(186..261)
                     /locus_tag="ACHK49_003621"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(226..228),aa:Lys,seq:ttt)
     gene            complement(264..339)
                     /locus_tag="ACHK49_003622"
     tRNA            complement(264..339)
                     /locus_tag="ACHK49_003622"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(304..306),aa:Val,seq:tac)
     gene            complement(475..550)
                     /locus_tag="ACHK49_003623"
     tRNA            complement(475..550)
                     /locus_tag="ACHK49_003623"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Lys,seq:ttt)
     gene            complement(715..1506)
                     /gene="cpoB"
                     /locus_tag="ACHK49_003624"
     CDS             complement(715..1506)
                     /gene="cpoB"
                     /locus_tag="ACHK49_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO9264203.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1516..2037)
                     /gene="pal"
                     /locus_tag="ACHK49_003625"
     CDS             complement(1516..2037)
                     /gene="pal"
                     /locus_tag="ACHK49_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO9264204.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2072..3364)
                     /gene="tolB"
                     /locus_tag="ACHK49_003626"
     CDS             complement(2072..3364)
                     /gene="tolB"
                     /locus_tag="ACHK49_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO9264205.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3497..4762)
                     /gene="tolA"
                     /locus_tag="ACHK49_003627"
     CDS             complement(3497..4762)
                     /gene="tolA"
                     /locus_tag="ACHK49_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO9264206.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4827..5255)
                     /gene="tolR"
                     /locus_tag="ACHK49_003628"
     CDS             complement(4827..5255)
                     /gene="tolR"
                     /locus_tag="ACHK49_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO9264207.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5259..5951)
                     /gene="tolQ"
                     /locus_tag="ACHK49_003629"
     CDS             complement(5259..5951)
                     /gene="tolQ"
                     /locus_tag="ACHK49_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO9264208.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5948..6352)
                     /gene="ybgC"
                     /locus_tag="ACHK49_003630"
     CDS             complement(5948..6352)
                     /gene="ybgC"
                     /locus_tag="ACHK49_003630"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO9264209.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6502..6795)
                     /gene="ybgE"
                     /locus_tag="ACHK49_003631"
     CDS             complement(6502..6795)
                     /gene="ybgE"
                     /locus_tag="ACHK49_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO9264210.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6795..6908)
                     /gene="cydX"
                     /locus_tag="ACHK49_003632"
     CDS             complement(6795..6908)
                     /gene="cydX"
                     /locus_tag="ACHK49_003632"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO9264211.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6923..8062)
                     /gene="cydB"
                     /locus_tag="ACHK49_003633"
     CDS             complement(6923..8062)
                     /gene="cydB"
                     /locus_tag="ACHK49_003633"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO9264212.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8078..9646)
                     /gene="cydA"
                     /locus_tag="ACHK49_003634"
     CDS             complement(8078..9646)
                     /gene="cydA"
                     /locus_tag="ACHK49_003634"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO9264213.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         2439 a         2713 c         2376 g         2432 t
ORIGIN      
        1 gtaagggagg tgctctccca gctgagctaa tcacccgata ctgcgctgga tactacaaac
       61 tacttccacc gaacccaccg taattagtgg tgggtcgtgc aggatgactc gcttcgctcg
      121 ccctacgggc cgttgctgac gcaacgttat cctacacgct taacatctgt cttcgatgct
      181 aaaactggtg ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag tcaactgctc
      241 taccaactga gctaacgacc cggtggtggg tgatgacggg atcgaaccgc cgaccccctc
      301 cttgtaaggg agatgctctc ccagctgagc taatcaccca atactacgct ggatactaca
      361 actaactatg ttagtggtgg gtcgtgcagg atgactcgac ttcgtctcgc ccttcgggcc
      421 gttgctgatg caacgttatc cttcacgttt tactaccgct tccaccttag cgattggtgg
      481 gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt caactgctct accaactgag
      541 ctaacgaccc actttttcgt tgctttcggt ttgtttgata tcccgtggca acggcggcat
      601 atattactga tttcagactt gagcgcaaca aaaatttcga tgcagatcac tcaactgctt
      661 atgattcgca cgacacgacc agaaataatg cgacttctgg tcgtgtgtta tgcattacat
      721 cgcgttcaga cgtttttgtg cctgtttagc gccatcagta ccagggtatt tactgataac
      781 ctgctggtat acggctttcg cttttgcggt gtcacctttg tcctgcatga tgacgccgac
      841 tttaaacatc gcatcagcag cctttggtga cttcggatag tttttcacta ccgaagcaaa
      901 atagtacgcc gcatcatctt ttttaccctt gttgtagttt aactgaccca gccaataatt
      961 ggcgtttggc aggtaagttg aatcagggta atttttgatg aaattctgaa atgccaccat
     1021 tgcgtcatcc tggcgggatt tatcctgcac cagcgcaata gctgcattgt aatccgtgtt
     1081 tgcatcgccg cttttcaccg gcgcgccagc attcgcagta ccagcatcag ctgtcggcgt
     1141 cgttgatgcc gccgcaccgc tttgatcgct gctggttgat tgcgccgctg caccaccgct
     1201 gctgaggctg tcgatctgca acaggatctg cttttgccgc tccacgacct gattcagttg
     1261 atactgattt tcctgaatct gaccacgcag ggaatcaata tcggattgat tatcagaaag
     1321 ttgttgctgg agttgggtta aaagctggct gtgagcatta gaaatacgct caagttgagt
     1381 gacgcggtct tcgaccgagc ctgagccgac actactgatt ggtgcctgag caaaagcggc
     1441 ccagggggcc gctataccaa ccagtaacga cagactcaat agttgatgtc tgaagttact
     1501 gctcatgcaa ttctcttagt aaaccagtac cgcacgacgg tttttggagt atgccgcttc
     1561 gtcatgaccc agtactgcag gtttttcttt accgtaagaa acgatggaga tctggtctgc
     1621 agaaacgcct ttaccctgca ggtacatctt aacggcgttc gcacgacgtt cacccaggga
     1681 gatgttgtat tccggagtac cacgttcgtc cgcgtgacct tctacggtga ctttgtaaga
     1741 cgggttgcta cgcaggaagt ttgcatgtgc atccagcatt tgagcgaagt cagaacggat
     1801 atcgtacttg tccagatcga agtaaacgat gttgttctgc tgcagctgtt gcatttgcag
     1861 acgagcctgc tcttcggaag acatgttgcc gttgccgccg ttcgcatcca taccagtgcc
     1921 ggcacccagc atgccttcgc tgccgtcatt gctggcgttc ttgttggaag aacatgccgc
     1981 aattgccata acaggcagag caatcatcag ccctttcagc actttgttca gttgcatttc
     2041 aatgattcct ttactattca attaattatt atcacagata cggcgaccag gcagggaatt
     2101 tgacctgtcc atcagttgcc ggaagacgcg ctttgaaacg cccatctgta gaaaccaaat
     2161 tcagcacgga tcccatcccc tgagaagagc tgtagattac catagtgccg ttaggtgcca
     2221 gacttggcgt ttcatccagg aacgtggacg acagaacttg tacgcctccc gttgccagat
     2281 cttgtttggc aatgtgctgc tgcccaccat tggagctgac cattaccata aatttaccgt
     2341 cgctgctgac atccgcatcc tggttctgcg aaccttccca ggtaatacgt tgtggcgcac
     2401 cgccgttgat attcacttta taaacctgcg gacgaccggc ctggtcagaa gtaaatgcca
     2461 ggttctggct atccgggaac caggtcggtt cggtattgtt actgcgacca tcagtcacct
     2521 ggcggatctg accagaagcc aaatccatta cgtacaggtt cagactaccg gttttcgaca
     2581 aggcgaatgc cagtttgctg ccgtctggcg agaatgcagg tgcaccgttg tgacgcggga
     2641 atgaagccac ctgacgtaca gcgccatttg ccagcgtctg aataaccagc gcggaacgac
     2701 cgctttcgaa ggtcacataa gccagtttag aaccgtctgg tgaccacgcc ggagacatca
     2761 gcggctgcgg tgaacggtga acgacaaact ggttgtaacc gtcatagtca gatacgcgca
     2821 gttcatacgg gaactgaccg ccgttggtct gaacaacgta ggcaatacga gtacggaacg
     2881 cacctttaat gccggtcagt ttttcaaaca cttcatcact ggcggtatga ccagcataac
     2941 gcagccactg cttgttcact ttgtacgagt tctgagcaag tacagtaccc ggtgcgccgc
     3001 cagtgtcaac aagttgataa gcaacattgt aagaaccatc cggattcgga gtgacctgac
     3061 cgacaactac agcgtcaatg cccagtgcgg accatgcagc tggttgtact tcctgcgcac
     3121 tacccggctg ctgtggcaga cgagcgcgat ctaacggatt aaatttaccg ctgttacgca
     3181 agtcagcagc aacgatgccg ccaatatctt caggtgccgc accaggcccc gcccactgga
     3241 aaggaacaac accaatagga cgaccggaat ctacaccgct gtcgatcaca atgcggactt
     3301 cagcatgcag aactgatgcc cacagtatga gaaaaccaaa tgctactcgt aatgcctgct
     3361 tcatcatatc tcccttatct ggaccgatgg cccacgataa tttagcagaa tgttaacaaa
     3421 ctaaaataca caaaactacc agaaccccgt ggcaaccggt gcctgatgtt gaccgtccga
     3481 acagtcaaca tcgcgattac ggtttgaagt ccaatggcgc gtttttgaac acttcatata
     3541 ctgcctggct tggtggtttc gggatcttcg caagtttagc tgctgccaac gcagcctgac
     3601 aaagtgcggg atcgccacct tcaggtttga tatccagtaa cataccatcg ggtgccagtt
     3661 ttatgcgcag cgtacaggtt ttgcctgcat aggacgatgc gtcatagaac ttactttcga
     3721 tagcagattt aatctgcccg gcatagttat tgatatcggc ccctgatgcg ccattgtttt
     3781 tagtattacc actcccggca ggcgaagcat tgttcccttt cgcccctccc cccgttttcg
     3841 gtgcattctt accagagctt agctcaccga aaatatcatc tgcctctgcg gccgcttttg
     3901 ctgcagcggc tttttcagct gcagcttttg ccgctgccgc ttttttgtct gcggcggctt
     3961 tttctgccgc tgctttctta tcagctgctg ccttttcagc agccgctttt tctgctgctt
     4021 ttttgtcggc tgcagctttc tctgctgccg ctttcttatc agctgcagcc ttttcagcag
     4081 ccgctttctt ctcagcttct gctttggctt tttcagcagc ttcagttgcc gctttctttc
     4141 ttgcttcagc tgcagctgct tctgccgctt ccgctttctt cttcagtgcc gcagcggctg
     4201 cctcggcttt tttctgcgct tcggctgcgg ctttggcggc ttctgcttct gcttttttct
     4261 ttgcgtctgc agccgctttc ttcgctgctt cttccgcagc tttagcatct gcttcggcct
     4321 tcgctttagc atctgccgcc gctttcgctg ccgcctcttc agcttgcttc tgctttaact
     4381 cggcctgttt tgcggcttct tcagcctgct ttttctgctc ctgagccgct aaccgctctt
     4441 tctcaagttg cttcaggcgt tcctgttcag ccgcttgttt ctcacgcagt tcttcagcag
     4501 cctgctgttc cttcatcttg cgctgttcat cagaacgctt cgcgcttgat tcctggcttt
     4561 gcatgcgttt gtactgctca actaccgcac ctgaatcaac catgacagcg tcgatggacg
     4621 aaccaccgcc gcctccggct gaagcttcta tattctcatc gaacgaactc cagatcagcg
     4681 ccgcaaataa gatgacatgc agcactgctg aaataattat cgcccgcttg agcttgtcgt
     4741 tttgttcggt tgcctttgac actctcggtt tccaaaaact gttcgcctgt tacccgctct
     4801 ctttcaagca agggaaacgc agatgtttag ataggctgcg tcattaaacc aaccgatttc
     4861 acacccgcac tatgtaacaa gttcagtgct ttaattattt catcgtaagg cacatctttt
     4921 gcgccaccga tcagaaagac cgttttcggg ttggccttga aacggctgga cacttccgcc
     4981 accacctgct ctggtggtaa acgctccagg cgatctttct caaccaccac ggtgtactga
     5041 ccaataccag acacttcaac aatcactggc ggattatcgt tactgctcac cgcctgtgat
     5101 tcagtagcgt ctggcagatc gacctccacg ctctgggtga tgatgggcgc tgtcgccatg
     5161 aagatcagca acagcaccag cagtacgtcc agcaacggta caatgttgat ttcggactta
     5221 agttcgcgac gacctcgtcc acgcgctctg gccatgactt accccttatt gctctcgcta
     5281 acggtaaacg cctggcggtg cagaatcgcg gtaaactctt ccataaagtt gtcgtaattc
     5341 agttccagtt tgtttacgcg ctggttgagg cggttgtagg ccataactgc cgggatagcg
     5401 gcaaacagac caattgcagt cgcaatcaac gcttctgcga taccgggcgc aaccatttgc
     5461 agtgttgctt gttttaccgc cccgagggcg ataaaggcgt gcatgatccc ccagaccgta
     5521 ccaaacagac caatatacgg gctgatggag ccaaccgtac cgaggaacgg aatgtgcgtt
     5581 tccagatttt caagttcacg gttcatggag atacgcatag cacgcgacgc cccttccact
     5641 acggcttccg gcgcatggct attggcacga tgcaggcgca caaactcttt gaacccgctg
     5701 taaaatattt gttccgaacc agtcagatta tcccgtttcc cctgactctc ttgatagagg
     5761 cgagagagtt cgattccaga ccagaattta tcttcaaacg cttcggcttc gcgcgccgct
     5821 gcgttaagaa tacgggtccg ctggataata atggcccagg atgcgattga aaaaccaatc
     5881 aaaatcaaca tgataagttt aaccagaagg ctagccttca ggaacaaatc aaggatattc
     5941 atgtcagtca ctgcttaaac tccgcgacaa tagacttggg aagcgcacga ggcttcattt
     6001 tgagtgggtc aacgcaaaca accagaacct ctgcttcatt cagcaaagta ttctcggcgt
     6061 tgacaatacg ttgcgtgaaa accaaagagg tgccacgcat tgatgttatt tcagtctgta
     6121 tttcgagcat atcgtcgagc cgcgcaggtg cgtaatattc caccgtcatt ttacgtacca
     6181 caaaggcaac gcgttcagcc atcagcgcct gctgactgaa gtgatgatga cgcagcatct
     6241 ctgtgcgtgc tctttcataa aaagcgacgt aactggcgtg gtacaccaca ccaccggcat
     6301 cggtatcttc atagtagacg cgaaccggcc atcgaaacag cgttgtattc actttacatc
     6361 ccggtaatgc aacaaaagtt agagctttta aacgctgcta ctatacgcgc gcgattgagg
     6421 tttgggaatg agggcaagtt aagggagcgt aaaaatttat gggcctggag cggcccataa
     6481 ttttttaaag agaattctga tttaaaagaa gaagaaaatc agcccgacaa tgagaacaat
     6541 atcggcaagc aatgggcaaa aaatcccttg ccaaagaacc ttctgcggac gaaagcccac
     6601 gccgtgaatc acaccagcac agacggccca catcagcaat aaaccatgcc agatttccag
     6661 ttcactggtc ttcgcggcaa aacgagatgg gtcccaaaac atacatcctg ctaacagaag
     6721 cgccatcacg aaggaaagcg cccgcagggg gcgcttgtcc attaccgcat acaaagtcgc
     6781 gataatctta ctcatcagat gtcttcttga ccggctttgc ctgattcgac gtgttcaagc
     6841 gccagcgcgg tgattacccc aaacgaacag gcaagaagcg ttcccagaat ccatgcgaaa
     6901 taccacattt ttagctcctt acttagtaca gagagtgggt gttacgttca atatcttctt
     6961 tggtgatacg accgaacatt ttccagtaac accaggcggt gtagagcaga atgatcggta
     7021 ccagaaccac cgcaacccag gtcatgacgt taagcgtcag ctggctggaa gttgcatccc
     7081 acattgtcag acttgcgttc atcatggtgc tggacggcat cacaaacggg aacattgcga
     7141 taccggctgt caggatgatg caggccagcg tcagggagga gaacacaaac gcccacgcgg
     7201 ctttatccat acgtgcagtc aggatggtca gcagcggcag aaccacaccc agtgccggaa
     7261 tagcccacag aattggcgtg ttgttgaagt taaccagcca tgcgccagct tcacgaacca
     7321 cttctttatt cagtgggtta gaggctgcgt aatggtccat tgtcgatttc acgacataac
     7381 catcgatacc gtacatcacc catacgccag ccagtgcgaa acagaccagt gtcaccagcg
     7441 cagccacctg agccgttgca cgggtacgca ggtgcagttc gcccacggta cgcatttgca
     7501 gataggttgc gccctgagta atgatcatcc ctacgctcac cacgcctgcc agcaggccga
     7561 acgggttaag caactggaag aagttaccgg tgtagtacag acgcagatat tcatcaacgt
     7621 tgaacggtac gccctgcaac aggttaccga acgctacacc aattaccagc ggcggaacga
     7681 agctaccaat gaagatgccc cagtcccaca tgttacgcca gcgggtttct tcaatcttgg
     7741 agcggtagtc aaaaccgacc ggacggaaga acaaagacgc cagcacgagg atcatcgcca
     7801 catagaagcc ggagaacgca gcggcataga ccatcggcca ggcagcaaag agtgcgccgc
     7861 ccgcggtgat cagccaaacc tggttaccgt cccagtgtgg tgcaatggag ttaatcataa
     7921 ttcgacgctc ggtgtcgtta cgaccgagga aacgggtgag catgcccacc cccatgtcga
     7981 aaccgtcagt gactgcaaaa ccaatcagca gaacgccaac cagcagccac cagataaaac
     8041 gcaatacttc ataatcgatc atttgacgac tcctgtctta gcgtgccggc tgagtagtcg
     8101 tggaagactg ctcaaagtga tagcgaccgg ttttcaggct gcttgggccg aggcgtgcaa
     8161 atttgaacat taagaacaat tctgccacca ggaacagggt atacaggccg caaatcagca
     8221 ccattgagaa gatgagatcg cctgcggtca gtgacgagtt cgccacagct gtcggcagca
     8281 cttcaccgat agcccacggt tggcggccat attcagccac gaaccagccc gcttctacag
     8341 caatccacgg cagcggaata ccgtacagcg cggcgcgcag aagccatttt ttctcgccaa
     8401 tgcggttgcg gatgacactc cagaaagaga gcgcgatgat tgccagaagc aggaagccac
     8461 acgccaccat gatacggaac gcaaagtaca gcggcgctac acgcgggatg gagtctttgg
     8521 ttgcctgttg aatctgcgct tcagtcgcat cagccacgtt tggcgtatag cgtttcagca
     8581 gcagaccgta accgaggtct ttcttcatgc tattgaactg gtcacgaacc gcctggtcgg
     8641 tagaaccaga acgcagttgt tcgagcagag agtacgcctt catcccgtta cgaatgcgtt
     8701 cttcatgctg caccatcagc tctttcaggc cgataaccgg ggtatccacg gaacgcgttg
     8761 caatgatgcc cagtgcgtaa gggatctgaa tcgcaaattt gttcgtctcc tcttcctgat
     8821 caggaatgcc gaacagagta aaggcagcag gcgcaggttg cgtttcccac tcggcttcaa
     8881 tagcagccag tttggttttc tgcacgtcgc ccatttcgta gccggattca tcacccagaa
     8941 caataacaga cagaacagca gccataccga agctggcagc gatagcaaag gagcgtttag
     9001 cgaaggcgaa gtcacgacct ttcagcatat accatgcgct gataccgagg atgaacatcg
     9061 cgccagtcac ataaccagac gctacagtgt gaacgaattt cacctgagca accgggttaa
     9121 gcaccagctc ggagaagctc accatctcca tacgcatagt ttcaaagttg aaatcggacg
     9181 cgattgggtt ttgcatccag ccgttcgcaa ccagaatcca cagtgcggac aggtttgaac
     9241 cgagcgccac cagccaggtg acacacatat gctgaacttt acccagacga tcccaaccga
     9301 agaagaacag acctacaaag gtggattcga ggaagaaggc catcagacct tcgattgcca
     9361 gcggcgcacc gaagatatcc cctacatagt gggaatagta agaccagtta gtcccgaact
     9421 ggaactccat ggtcagaccg gtagccacac ccagagcgaa gttgatacca aacaacttgc
     9481 cccagaactt ggtcatatct ttataaatct gtttgccgga gaggacgtag accgtttcca
     9541 taatggccag caggaacgcc ataccgagcg tcagtggcac aaaaaggaag tggtacatcg
     9601 cggtcaaggc aaactgtaag cgcgacagtt cgactatatc taacatcatg actccttgct
     9661 catcgcatga agactccgag agtgaacccc gttagaaagg gtcacacgca tgccccaata
     9721 gaaatttgtt tgaacacttt catcatacca tgtgaatgca cacaaaaacg gcgtaaagaa
     9781 aggttgcaaa catgttaata aaaactcaaa ttgatcccac gtatatatta cgccgcaaaa
     9841 tccttacaat aaacaggttt ttgttgaagc aaatttgcat ttttcgacgg tgatcaattt
     9901 atagctaaat taccgccttt cagccaattt gatcgagaac aatttatctc tttttgatgc
//
LOCUS       DAZGLJ010000167         9876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000167 DAZGLJ010000000
VERSION     DAZGLJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.167"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1181
                     /locus_tag="ACHK49_003635"
     CDS             <1..1181
                     /locus_tag="ACHK49_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9264214.1"
                     /translation="DNTGAEPADEAGSEQPEETAETVSPVQRLAQLTAELDALHNDRL
                     LVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWLGDTIKGQDLAIASLHKH
                     LLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINMSEFQEKH
                     TVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQAFDKGEM
                     ADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPALLARMEV
                     VPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVIIEPEVTDEIMSRVTRAENGARML
                     ESVIDGNMLPPLSLLLLQKMAANTAVARIRLSAVDGAFTADVEDAQNDESVTKDETVL
                     "
     gene            1178..1921
                     /gene="vasI"
                     /locus_tag="ACHK49_003636"
     CDS             1178..1921
                     /gene="vasI"
                     /locus_tag="ACHK49_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO9264215.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            1926..3338
                     /gene="tssA"
                     /locus_tag="ACHK49_003637"
                     /pseudo
     CDS             1926..3338
                     /gene="tssA"
                     /locus_tag="ACHK49_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
     gene            3447..6881
                     /gene="tssM"
                     /locus_tag="ACHK49_003638"
     CDS             3447..6881
                     /gene="tssM"
                     /locus_tag="ACHK49_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO9264216.1"
                     /translation="MLILAWIFLLVWIWWKGPMWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFITRYGLQSYFVKQRDELVELTAMDSWVLNLTRNVKYSDADRAEIQHQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            6892..8341
                     /locus_tag="ACHK49_003639"
                     /pseudo
     CDS             6892..8341
                     /locus_tag="ACHK49_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            8268..8750
                     /locus_tag="ACHK49_003640"
     CDS             8268..8750
                     /locus_tag="ACHK49_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO9264217.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            8794..9708
                     /locus_tag="ACHK49_003641"
     CDS             8794..9708
                     /locus_tag="ACHK49_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264218.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNAGKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIISSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
     gene            9718..>9876
                     /gene="ykfM"
                     /locus_tag="ACHK49_003642"
     CDS             9718..>9876
                     /gene="ykfM"
                     /locus_tag="ACHK49_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HFO9264219.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRML"
BASE COUNT         2407 a         2630 c         2726 g         2113 t
ORIGIN      
        1 aggataatac cggtgccgaa ccggctgatg aagctggcag cgaacagccg gaagagaccg
       61 ctgaaacagt ttccccggta cagcggctgg cccagctcac tgccgaactg gacgccctgc
      121 ataacgaccg gttgctggtc tccccgcacg tcgataaaaa acagattgcg gcggtgattg
      181 ccgaatggac cggcgtgccg ctcaaccgcc tatcgcagaa tgaaatgtcg gtcatcaccg
      241 acctgacgaa atggctgggt gacaccatca aaggccagga cctggcgatt gccagcctgc
      301 ataagcatct actgaccgca cgcgccgacc tgcgtcgtcc gggacgcccg ctcggtgcgt
      361 tcctgctggc tggccccagc ggtgtgggta aaaccgaaac cgtcctgcaa ctggcagagc
      421 tgctctacgg cggtcgccag tacctgacca ccatcaatat gtccgaattc caggagaaac
      481 acaccgtctc gcggctgatt ggttcgcctc cgggctacgt tggctacggt gaaggcggcg
      541 tactgaccga agcgattcgc cagaaaccct actcggtagt actgctcgat gaagtggaaa
      601 aagcgcaccc ggatgtgctc aacctgttct accaggcgtt cgataagggc gaaatggcag
      661 acggtgaagg ccgcctgatt gactgcaaaa atatcgtctt cttcctgacg tccaacctcg
      721 gctaccaggt aatagtcgag catgccgatg acccggaaac catgcaggaa gtactgtatc
      781 cggtgctggc cgacttcttc aaacctgccc tgctggcgcg tatggaagtg gtgccgtacc
      841 tgccgctgtc gaaagagacg ctcgccacca tcattgccgg gaaactggcc cgcctggata
      901 acgtgctgcg cagccgcttt ggcgcggagg tgattataga accggaagtg acggacgaaa
      961 tcatgagccg cgtcacccgc gcggaaaacg gcgcgaggat gctggaatcg gtcatcgacg
     1021 gcaatatgct gccgccgctc tccctgctgc tgttgcagaa aatggcggcg aatacggcgg
     1081 ttgcccggat tcggttgtcg gcagtggacg gcgcatttac ggcagacgtg gaagatgctc
     1141 agaacgacga gtccgtcaca aaggatgaaa cggttttatg agcaggttgc gcgttttccg
     1201 gcagacagga tggtttatcg cgggcctgat gacgggctta cccgccactg ccgcgcccgc
     1261 agaagcatcg tcaatggccg gtgtggcggt cgccgtcgcc accacgaccc cgccggatgc
     1321 gacagccacg ttacaggcca tgcagtcctg tcggcgggag tccgcggcgc tggagcggct
     1381 tgactgctat gaccgcctgc tggcaccgct gtccccgtca gggtttgacg gggcgctggt
     1441 caaagccggt tttgtgggcg aggcctggac ccgcgccaca gagcaggaaa aacgccgcga
     1501 gggcaacacc acagaactgc tggtgacgca ggtgccgggg gagcgcccga cggtggtgat
     1561 aaccaccccg gctatcggtc atgttccgcc acgcccggtg ctgatgttca gttgcgtgga
     1621 caatatcacc cggatgcagg tggcgctgat gcaccctctg gatgtccatg atatcgccgt
     1681 cactctgaat gccgacaacc gggcattgcg cagccactgg tttgtgcgcg agaacggcac
     1741 cctgctggag tccagccggg ggttatccgg gattgatgaa attaagcaac tgttcggcgc
     1801 aaaaacgctg acggtggata ccggcgcaga caatgccgcc ggaaagctga cttttaacat
     1861 tgacgggctg gcaagggcga ttgctcccct gcgcgatgcc tgccactggg cgggagaata
     1921 aaaatatggc aatggactta cgcgatccga atgtctggat atcgcacctg ctggaaaacc
     1981 tgccggaaga gaaactggca tcggcgctga aagatgacaa cccgaactgg gagtatatcg
     2041 acggcgaaat cgtcaagctg ggatcgctgg cccacagtca actggatatc ccggaactcc
     2101 agcgccgtgg gctggtcatt ctggcttcgg agagtaagga ttttcgtctg ctggcgcatc
     2161 tgctgagaac cctgcaacac gccggtgatc ctcatctcgc attgcgcctg ctggcgctgt
     2221 atgtggagca ttactgggcg gtggctgcgc cgcagaatat ggcgcataaa aaacgctttg
     2281 ccagccaggt cattaaacgc tttgaaacag gtattgaagt tttttcacag aacgccgcca
     2341 cagcgcagcg cgatgcgctg tcaggggaac tggcaaaact ggcgcaatgc tggcagtcac
     2401 ataacgcccc ggaactggca caggctactg atgatctttt tgccctgtac cagcgtgcgt
     2461 ttaatcgtgc ggcaccggct cctgtcccca ctccggcggc ctccggtagt tcaccacaga
     2521 ccaccgccac gtctgaaagc ggcgtgacgc aacccagtgc tccggctccc caaatagtca
     2581 tcgacagtca cgacgacaaa gcctagcgcg acacgctgtt aaaagtggcg gccattttat
     2641 gtgaacgcca gccggattca ccgcagggct accgtctgcg ccgccatgcg ctttggcaga
     2701 acatcaccag tacgccccag gcggaaagcg atggccgtac tccactggct gcggtctccg
     2761 ccgatatggt ggcagattac catgctcagc tcggtagtgc cgatatggcg ctgtggcagc
     2821 aggtggagaa aagcgtactg ctggctcctt actggctgga tggtcactgc ctttctgcac
     2881 agacggcgct gcgtctgggt tacaaacagg tggcagacgc catccgcgat gaggtcatcc
     2941 gcttccttga gcgtctgccc cagcttaccg ggctgctgtt taatgaccac acaccgttta
     3001 tcagtgagca gacgaaacaa tggctggcag cttcgcccga cgccaaagtt gcaccggttg
     3061 cgcaaatcgg tgaggaatcg aaggcagcca gagcctgttt tgctgagcag ggtctggagg
     3121 cggcgctgcg atatctggac atgctacccg aaggcgatcc ccgcgatcag tttcaccgcc
     3181 agtaccttgc cgcacagttg acggaagagg cggggctggt acagcttgcg cagcagcagt
     3241 accggatgtt gttcaggatg ggattgcaga tgatggtggc agactgggag ccgtccctgc
     3301 ttgagcagct agaacaaaaa ttcacggcag aacaataaaa gagaacagga gtttttgtgt
     3361 tcagattacc aacaccccga ctactcagcg gactcaaatc agccctgcga ccggcgatgc
     3421 cccggtttaa agtctctgct ttctggctgc tgatactggc gtggattttt ctgctggtgt
     3481 ggatctggtg gaaaggcccg atgtggacgc tgtatgaaga acagtggctc aaaccactgg
     3541 cgaaccgctg gctggcaacg gcggcgtggg ggattattgc cctggtgtgg cttaccgtcc
     3601 gggtgatgaa gcgcctgcaa cagcttgaaa aaatgcaaaa gcaacagcgc gaggaagccg
     3661 ttgatccgct cagcgtggaa ctgaacgccc agcagcgtta tcttgaccgc tggctgctgc
     3721 gcctgcaacg ccatctcgac aaccgccgtt tcctgtggca gttgccgtgg tatatggtca
     3781 tcggcccggc gggcagtggc aaaacgacac tgctgcgcga ggggtttccg tccgacatta
     3841 tttatgcccc ggagggcgca cgcggcgcag aacaacgcct gtacctcacg ccccatgtcg
     3901 gtaaacaggc ggtgatcttt gatatcgacg gcacactctg cgctcccgct gatgcggata
     3961 tcctgcatcg ccgtctgtgg gaacatgccc tcggctggct gaaggaaaag cgcgcacgcc
     4021 agccgctgaa cgggattatt ctgacactcg atttacccga tctgctgacc gcagacaaac
     4081 gccgccgcga gcatctgtta cagacgctgc gcagccgctt acaggatata cgccagcatc
     4141 ttcactgcca gttaccggtt tacgtggtac tgacccggct tgatttatta cagggttttg
     4201 ccgccctgtt ccagtcactc aacagacagg atcgcgatgc gattctgggc gtcacgttca
     4261 cccgccgcgc ccatgaaaat gatgactggc gaacagagtt gaatgctttc tggcagacat
     4321 gggtggatcg aatgaatctg gcgttgccgg atctgatggt cgctcagact cacacccgcg
     4381 cgtctttatt cagtttttcc cgccagatgc agggaagccg tgaaccgctg gtgtcactgc
     4441 ttgagggtct gcttgatggc gaaaatatga acgtgatgct gcgtggtgtc tatctcacct
     4501 cttcgcttca gcgtgggcag atggatgata tattcaccca gtctgccgcc cgccagtacc
     4561 ggctgggcaa taacccactg gcgtcctggc ccctggtgga caccgcgcct tatttcaccc
     4621 gcagcctgtt cccgcaggca ttactcgcag agcctaatct ggcaacagag agccgcgcct
     4681 ggctgatacg ttcccgtcgc cgcctgacgg ttttctctgc cacaggcggc gtggcagcac
     4741 tgctgctcat caccggctgg catcactatt acaacggtaa ctatcagtcc ggcatcaccg
     4801 tgcttaagca ggccaaagcc tttatggacg tgccgcctcc gcagggggaa gatgactttg
     4861 gcaacctgca actgccgctc ctgaacccgg tacgcgatgc cacactggcc tatggcgact
     4921 ggggcgaccg cagccgtctg gccgatatgg gactgtacca gggacgacgt atcgggcctt
     4981 atgtggaaca gacctatctg caactgctgg agcaacgtta cctgccctcg ctgtttaacg
     5041 ggctggtcaa agcgatgaac gccgcgccgc cggagagtga agaaaaactc gcggtgctgc
     5101 gcgtaatgcg aatgctggag gacaaaagcg gacgtaacaa tgaggtggtg aagcagtata
     5161 tggcaaaacg ctggagcgaa aaattccacg gccagcgcga tatccaggca caactgatgt
     5221 cccatcttga ctacgcgctg gctcatactg actggcatgc agagcgtcag gcgggcgacg
     5281 gtgacgccat cagccgctgg acgccatatg acaagcccgt ggtatcagca cagaaagaac
     5341 tgagcaaact gcctgtctac cagcgggttt accagagcct gaaaacgcgg gcgctgggcg
     5401 ttcttcctgc cgacctcaat ctgcgtgacc aggtagggcc aacctttgac caggtgttta
     5461 catctgccga tgacaacaaa ctggttgttc cacagtttat cacccgctac ggcctgcaaa
     5521 gctattttgt aaaacagcgc gatgaactgg ttgaactgac agcgatggat tcctgggtac
     5581 ttaacctcac ccgcaatgtg aaatacagtg acgccgaccg tgcggaaatc cagcaccagt
     5641 tgaccgagca gtatatcagc gactacaccg ccacctggcg ggctgggatg gacaatctga
     5701 atatccgcaa ttttgagtcc atcggacaac tgaccggggc gctggagcag gttatcagcg
     5761 gcgaccagcc tttgcagcgg gcgctgaccg tgctgcgtga caacacacag ccaggcgtct
     5821 tttctgaaaa actctctgcc aaagaacggg aggaagccct ggcagagccg gattaccagt
     5881 tactcacccg cctcgggcat gaattcgccc cggaaaacag taccctggca gtacagaaag
     5941 acaaagaaag cacgatgcag gccgtgtatc agcaactcac cgagttgcac cgctacctgc
     6001 tggcaatcca gaacgcgcct gtaccaggga aatcggcgct gaaagccgtg cagttacggc
     6061 ttgatcagaa cagcagcgat ccgatattcg ccacccgcca gatggcaaaa acgctgcctg
     6121 ctccgctcaa ccgctgggtt ggcaggctgg ctgaccaggc ctggcatgtg gtgatggtgg
     6181 aggctgttca ttatatggaa gtggactggc gcgacagcgt ggtgaaaccg tttaacgagc
     6241 aactggcaaa taactatccg tttaatccgc gttctgcaca ggatgcctca ctggatgcct
     6301 tcgaacgctt ctttaaaccg gatggcatac tggatacctt ctaccagcag aacctgaagc
     6361 tgtttatcga taatgacctg agtctggagg atggcgataa caacgtcatt attcgcgaag
     6421 atattattgc gcaactggaa actgcgcaga aaatccgtga catcttcttc agcaaacaga
     6481 acggtctggg aacatccttt gccgtggaaa cggtatcgct ttcaggcaat aaacgccgca
     6541 gtgtactgaa ccttgacggt cagttagtcg attacagcca gggccgtaac tataccgccc
     6601 atctggtctg gcctaacaac atgcgcgaag gcaacgaaag taagctgacg ctcatcggca
     6661 ccagcggcaa cgcgccgcgc agtatcagct tcagcgggcc gtgggcgcag ttccgcctgt
     6721 tcggggccgg acaactgacc ggagtacagg atggcaactt taccgtgcgc tttagcgtgg
     6781 acggtggcgc gatgacctac cgtgtgcata ccgacacgga agataacccg ttcagcggtg
     6841 ggttgttcag ccagtttggt ctgtcagaca cactgtactg aggaggcagg gatgaatagt
     6901 aacgtactga cacaaactat cgttaccggc agtgacccgc gcgggctgcc ggaattcagc
     6961 gccatccgcg aggaaataaa caaagccagc cacccgtcac agcctgagct gaactggaaa
     7021 ctggtggagt cgctggcgct ggcgattttt aaagccaacg gtgtggattt acacaccgcc
     7081 acctactata cgcttgcccg gacacggaca cagggactgg cgggattctg cgaaggtgcg
     7141 gaactgctgg cggcaatggt aagccacgac tgggataagt tctggccgca gggcggcccg
     7201 gcgcgtactg aaatgctgga ctggtttaac tcccgaaccg gcaatattct gcgtcagcaa
     7261 atctcctttg cggaatccga cctaccactg atctaccgca cagagcgggc attgcagctt
     7321 atctgcgata agctccagca ggtggaactg aagcgcgttc cgcgcgtgga gaatctgctc
     7381 tattttatgc agaacacgcg taaacggctt ggaccccagc tgaagagtaa cactgagaac
     7441 gccgcacaga ccacggtcag aacgctgatt tatgccccgg aaacacaggc atcttccaca
     7501 ccagaagcgg tagtgcctcc cctgcccggc ctgcctgaga tgaaagtgga agttcgcagt
     7561 ctgacagaga atcccccaca ggccagtgtg ataaatcaag gcagtacggt aagagggttt
     7621 atcgcaggga tcgcctgttc agtggctgtc gcctcagcat tgtggtggtg gcaggtctat
     7681 ccggtgcagc agcaactgtt acaggttaac gacaccgctc agggcgcagc aacggtgtgg
     7741 atggcctcac ctgaactcga aaactatgag cgcaggctgc aacaacttct tgatacctcc
     7801 ccggtacagc cgctggaaac cgggatgcag atgatgcgtg ttgccgacag tcgctggccg
     7861 gaaagcctgc aacagcaaca ggcctcgaca caatggaatg aggcactcaa aacccgcgca
     7921 cagagtagcc cgcagttgcg tggctggttg cagacccgcc aggacttaca tgcttttgca
     7981 gatctagtga tgcagcgcga gaaagaggga ctaacccttt cctatatcaa aaatgtcatc
     8041 tggcaggcgg agcggggact ggggcaggaa acacccgttg agtctctgtt gacgcagtac
     8101 catgatgccc gtgcgcagaa gcagaataca gatgcgctgg aaaaacaaat taatgagcga
     8161 ctcgaaggcg tgttaagccg ctggctgctg ctgaagaata acgtcatgcc agaggcggca
     8221 accggtacca cggccgaaaa ataattattt aattaaagga gattatcatg gcaaatataa
     8281 gttatttatc tttgagtgga gagacacaag ggctaatttc agcaggttgc tcaacgcttg
     8341 attctgtcgg aaacaaagcg cagccagaac ataaagatca gatcatggtt tatgcgttaa
     8401 tgcattccat ctcaagaagt caaaatgtca atcatcatga attaattatc accaaaccgg
     8461 ttgataaatc ctcaccattg ctggcaaaag cacttagtga caatgaaaaa atggcaatat
     8521 gtgaatttat actctaccga accagtaaag cgggtattta ccagccttat tataaaataa
     8581 atctgtcaaa agcccgaata tcaagtattg attttgtcac accgcatgct gtcctggaaa
     8641 aagaactgga accacaggag agaattgcct ttatttatga agatatttcc tgggagcata
     8701 cgttagccgg tacgaatgca atgagtaaat ggcaggatcg tgtacagtaa cttactgtaa
     8761 taacataata aattactatt gcggagagat aaaatggctg gtttaaggaa taacaacaac
     8821 acacagaatg cacagtgggc tgattatgtg ggtgacattc ttcggggggc tcagccgata
     8881 aaccagctag tacctcagca cccttatctt aacgacgtcc cactcattga tgagttaagg
     8941 catcagaaca ctcatcatgt tattctcttg acacttgatg ttgcaaaaaa aatactcagc
     9001 ccaataacat catttgatta cattcatttt atcactacac atccttcagg tataaaagat
     9061 acactcgcct ggttagttaa cgcgggtaaa ttaatgactg agtttgatga caatgggaaa
     9121 attatattta accttaatgc cttaaagtat acaaaagcaa gttactttga aatacttgga
     9181 gagaaatata taaaaatcac aacatccagc ccctggcttt tagaaaagct cggtaaatat
     9241 attttctctt cacgagcgcc tcaagttctg gaactcgcta ttggctggcg aggagcttta
     9301 tctgaatcaa taaaaggagt taagttctgt atctggtttt cagtagcctg gagaaccata
     9361 gaatttatta taagttcaga gcgagatctg gttaattttc ttggcgattt ttcaatggat
     9421 gtggccaaag cagttatagc cggtggtgtt gcaaccgcta ttggaagcct ggcttctttt
     9481 gcctgtgtta gctttggctt tccagtaatt cttgtcggag gagcaatttt actgacaggg
     9541 atagtgtgta cagttgtttt aaatgaaatc gatgctcaat gccatttatc agaaaaatta
     9601 aaatatgcaa ttagagatgg actaaaacgg caacaggaac ttgataaatg gaaaagggaa
     9661 aacatgactc catttatgta tgttcttaac actccacccg tgatatgagg attaaccatg
     9721 cgtttacatg ttaaattaaa agagtttctt tcgatgtttt ttatggccat attatttttc
     9781 cctgcattca atgcttcttt gttttttacc ggagttaaac ctctatattc aataataaaa
     9841 tgctccacgg aaatatttta tgattggaga atgttg
//
LOCUS       DAZGLJ010000168         9818 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000168 DAZGLJ010000000
VERSION     DAZGLJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.168"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..524)
                     /locus_tag="ACHK49_003643"
     CDS             complement(<1..524)
                     /locus_tag="ACHK49_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HFO9264220.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAV"
     gene            complement(521..2209)
                     /gene="yfaA"
                     /locus_tag="ACHK49_003644"
     CDS             complement(521..2209)
                     /gene="yfaA"
                     /locus_tag="ACHK49_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO9264221.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(2358..4985)
                     /gene="gyrA"
                     /locus_tag="ACHK49_003645"
     CDS             complement(2358..4985)
                     /gene="gyrA"
                     /locus_tag="ACHK49_003645"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO9264222.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            5132..5854
                     /gene="ubiG"
                     /locus_tag="ACHK49_003646"
     CDS             5132..5854
                     /gene="ubiG"
                     /locus_tag="ACHK49_003646"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO9264223.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            complement(5983..9717)
                     /gene="yfaL"
                     /locus_tag="ACHK49_003647"
     CDS             complement(5983..9717)
                     /gene="yfaL"
                     /locus_tag="ACHK49_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO9264224.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGVVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLAIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVVDAGDGQVSLANN
                     NSYLGTTQIASGMLEVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         2283 a         2732 c         2373 g         2430 t
ORIGIN      
        1 ctgactgcgc gcattccgtt gtcagttttc gtggcgcttc cggtgtggta gatgacgtaa
       61 cgccccatcc agaccattaa gtgctgggca tcgccctgat cgaaaaaaat catatcgcca
      121 ggcagcgcct ggtttatgtc ctggccaata aactggctgt tgtactgaat caaattaatc
      181 gcggtcacgt aggggccggt tttcccgttc ccctgattcc agttttgcgc cagttgacgc
      241 tgtccaggtg ttagcgtcat ctctggcggc aaatactggc tagagaaacc gttacttttt
      301 agccatttac tatcgtgaac tttcagcgtc tcgttcgccg caaatcgcac caggcccgca
      361 caatcctgct gataccagcg tggacttggc ccctggcgga gctgttcttg tgcaatgcgt
      421 acaaaccagg cgcgaaacag cccggattgt tcgacattca gcatttcgct atgggcaaca
      481 acacaacaca gccagcaaat cagcgccagc agcccgtgcc tcatagcggc tgccaggtta
      541 tgggtagcca ctgccaggcg gcaccgggtt ccatctgggc cagcttcatg acataacgcg
      601 gttgttgaga taaagcgtcc agcttcggca ttaataaagt ttgtgcggcg ttataaaaaa
      661 ccggttcgag attcttcggc agactggtca acgtttcgtt acgcagcagt ttcgccacgc
      721 cttgtggatt gatatagagc ggaacgatgc catcagtggg tattacatcc accattgccg
      781 ggcggttttt attcagtgtt tgcagggcgt tattaaccag cgtgtcatcg agggagaaaa
      841 gcagcgtttt gttttgcatc gccagcgaca cgcggaaaaa ataatccgac attaattgat
      901 cgggttgcgc cgcctgcgct ttcggatact gaccgtatcg ggaactcact tcgcgacgcc
      961 agatttgcgc ttcgccctgc tgagtctggc ttatcggcaa aacaccttct ggcgctttgc
     1021 tttcgtgcgc accaatattt tgcgtaaaca gtttccctgg caattgcgcc tgttcggcag
     1081 tgccatcaaa ctgaccgaca aacagcgggg tttgcaattt tgagtctgca taccagcaca
     1141 gccccgcggc accgtctaac gccccattca acttgtcgtt ttcctgactg atgtgcgaaa
     1201 gcatctcttc ggcaataccg tgtgaatacg gcaccaccac acagaagctg gccccggcag
     1261 gcatactgtt ccagaccggc gtaaaatcga aactggtatc tacgctggcg gattcatcat
     1321 ttaacgccac aaaactgtgc cagccgtcgt tacccatttc gaagcgtacg ccagcaaaag
     1381 aaggcattaa ccgctggtag ccaaaaccca gcaacctggc gctgaccacg atgcgctggc
     1441 gtactggcgt tttttcagca gtacgctctt ccaggccaaa gcttgcttgc cagcgttttt
     1501 tgccgctcaa caaatcacct gcgatcgccg tggcttcggt atcctgctga tcatctttaa
     1561 acaacatatc cgtgctggaa aacaccagca tcttgtcctg ataagtcgcg aacatcaggg
     1621 cgttattgcc gttatagcgc aactgataaa caggaacagt ttcactattt atcttgatgc
     1681 tactgatttc cgttttgctt aactggctgt cgctggtagc ggcaaacaac aatggctcca
     1741 gcagtttgct taaaccactg cgctggatca gcaccatata atgtgaaaga tggccttgtt
     1801 tatcgtgcca cagcgctgcc tgcgcgggct gatctaagag tgacgaaaag agcttatctt
     1861 tcagcgtgag atcgtgttca tagacaatac gacgaatgct gccttcaatg cccagacgat
     1921 cggcatgatt ctgataatag aaaacgaaat cttcgctcag aacatcgtgg agaaacggaa
     1981 tggtgaggag atctttggga agctggctca gagagtcgct gtcgagaaag aggtccggct
     2041 cattgagatc gatttgcaga ttgttgtgca ccaccagcgg cgacaacgtt ttttctggcc
     2101 cactgccagc atattgcaac gcccagacgc cagcggaaag cagtgctatt gcgccaaaac
     2161 ctacaagacc atagaaccgc cagcctttcg cctttttttc accactcatt gccacattcc
     2221 ttgtgtatag ccagccattt tttacgggca cagccaaact ttaccgtgcc ctaatacgac
     2281 aaaagcccag actttgcagc ctggactttt caattcaaac aagggagata gctccctttt
     2341 ggcatgaaga agtaaaatta ttcttcttct ggctcgtcgt caacgtccac ttccggagcg
     2401 atttcatcgt ccccttccgc ggcactgcca tcgatggtat ccagatcttc ctcgtcaacc
     2461 ggttcagcaa cacgttgcag acccactacg ttttcatctt ccgcagtacg gatgaggatc
     2521 acgccctggg tgttacggcc tacgatgctg atttccgaaa cgcgagtacg taccagcgta
     2581 ccggcatcgg tgatcatcat gatctggtcg cagtcatcta cctgtaccgc gccaaccact
     2641 aaaccattac gttcagtaac cttaatggag ataacccctt tcgtcgcacg cgacttggtt
     2701 gggtattccg ccactgcggt acgtttaccg taaccgtttt gcgttgcggt gaggattgcg
     2761 ccatcgccac gaggcacgat cagagagacg actttatcgc cttcacctaa gcgaataccg
     2821 cgaacaccgg tggtgttgca gcccatcgca cggacagaag actctttaaa gcgcaccact
     2881 ttaccttcag cggagaacag cattacttcg tcttcgccgc tggtcaggtc aacgccgatc
     2941 agctcatcgc cgtcaaccag tttgatcgcc actttaccgg cggtacgcag acggttgaac
     3001 tcggtgagga cagttttctt cacggtaccg ttagcggtcg ccatgaagac tttcacgcct
     3061 tcttcaaact cggtcactgg caggatcgca gtgatacgtt cgtcctgctc cagcggcagc
     3121 aggttgacga tcggacgacc gcgcgcgcca cgagtggctt ccggcaactg ataaactttc
     3181 atcgaataga cgcgaccacg gctggagaag cacagaatat ggtcgtgagt gttcgccacc
     3241 agcagtcggt cgataaagtc ttcttcttta atacgtgcgg cagatttacc tttcccgcca
     3301 cgacgctgcg cttcgtattc agaaagcggc tgatacttaa cgtagccctg gtgagagagc
     3361 gtcactacca catcttcctg agtgatcaga tcttccaggt tgatgtctgc gctgttggcg
     3421 gtgatttcag tacgacgttt gtcaccgaac tgttcacgaa ccagctccag ctcttcacgg
     3481 atcacttcca tcaaacgatc ggcgctacca agaatacgca acagttccgc gatctgatcc
     3541 agcagctctt tgtattcgtc gagcagtttt tcgtgctcaa ggccggtcag tttctgcaaa
     3601 cgcagatcca gaatcgcctg agcttgctgt tcggtcaggt agtacagacc atcacgcacg
     3661 ccgaactctg gctccagcca ttccggacgc gcagcatcgt cgccagcacg ttcgagcatc
     3721 gcggcaacgt tgcccagctg ccacggatta gcaaccagcg cagttttcgc ttctgcaggc
     3781 gtcggcgcat gacggatcag ttcgatgatc gggtcgatgt tcgccagcgc cacggctaat
     3841 gcttcaagga tatgagcacg atcgcgagct ttacgcagtt cgaaaatagt acgacgggtc
     3901 accacttcac ggcggtgacg aacaaacgcc gcgatgatgt ctttcaggtt catgatcttc
     3961 ggctgaccat ggtgcaatgc caccatgttg ataccgaaag aaacctgcaa ctgggtctgg
     4021 gagtagaggt tgttgagcac aacttcaccg accgcatcgc gtttcacttc aatcacgatg
     4081 cgcataccgt ctttgtcaga ctcgtcacgc agcgcgctga tgccttccac gcgtttttct
     4141 tttaccagtt ccgcaatctt ctcgatcagg cgcgctttgt ttacctgata cggaatttcg
     4201 tggacgataa tggtttcacg accggttttg gcgtcaactt ccacttctgc gcgagcgcgg
     4261 atatacacct tgccgcgacc ggtacggtaa gcttcttcaa taccgcgacg accgttaatg
     4321 attgccgccg tcgggaagtc cggccccggg atgtgttcca tcagcccttc aatgctgatg
     4381 tcttcatcat cgatatacgc cagacaaccg ttgatgactt ccgtcaggtt gtgcggcggg
     4441 atgttggttg ccatacctac ggcgataccg gaagaaccgt tcaccagcag gttaggaatt
     4501 ttggttggca tgacgtcggg aattttttcc gtgccgtcat agttatcaac gaaatcgacc
     4561 gtctcttttt cgagatcggc catcagttca tgggcaattt tcgccagacg gatttccgta
     4621 taacgcattg ccgccgcaga gtcgccgtcg atggaaccga agttaccctg accgtctacc
     4681 agcatgtaac gcagcgagaa tggctgcgcc atacggacga tcgtgtcata aaccgccgag
     4741 tcaccatggg gatggtattt accgattacg tcaccaacga cacgggcaga ttttttatag
     4801 gctttgttcc agtcattgcc tagtacgttc atggcgtaaa gtacgcgacg gtgtaccggc
     4861 ttcaggccat ctcggacatc tggcagcgca cggccaacaa tgaccgacat cgcataatcc
     4921 agataggagc tcttcagctc ttcctcaatg ttgaccggtg taatttctct cgcaaggtcg
     4981 ctcatctaac cgctatccct ctactgtatc ccggattcaa aggtcgcaaa ttataacaca
     5041 gccgcgcagt ttgaggtaaa cctatacgct ttattcacat ccaatgcctg atatactcgt
     5101 ttgtcttgcc aattacggag tagaagtgcc aatgaatgcc gaaaaatcgc cggaaaacca
     5161 taacgtagac cacgaagaga tcgctaaatt tgaagccgtc gcctcccgct ggtgggatct
     5221 tgaaggtgag ttcaaaccgt tgcaccgcat taacccgctg cgtctgggct atattgccga
     5281 gcgtgctggc ggtttatttg gcaaaaaggt gctcgatgtc ggttgtggcg gcggcattct
     5341 ggccgagagt atggcgcgcg aaggcgcgac ggtgaccggt ctggatatgg gctttgagcc
     5401 attgcaggtg gcaaaactgc acgcgctgga aagcggcatt caggtggatt atgtgcagga
     5461 aaccgtggaa gagcacgcgg caaaacatgc cgggcagtat gatgtggtga cctgcatgga
     5521 gatgctggag cacgtccccg atccgcagtc agtggtcaga gcctgtgcgc aactggtgaa
     5581 accaggcggc gatgtctttt tctcgacgct taaccgcaac ggcaagtcat ggctgatggc
     5641 ggtggttggc gcggaatata ttttgcgcat ggtgcccaaa ggcacgcatg atgtgaagaa
     5701 gtttattaaa ccggcagaac tgcttggctg ggtagatcag acaagcctta aagagcgaca
     5761 tataaccggg ctacattaca acccgctcac taatactttt aaactcggcc ccggcgtgga
     5821 tgtgaactat atgctgcaca cgcagaataa gtgaggttgt tgtttggccg agccaatgcc
     5881 tgatgcgacg cttgccgcgt cttatcagta tctacaaatg ctccccgtag gccggataag
     5941 gcgtttacgc cgcatccggc aaccgtgccg actagacagt gattaccatt tcaccgtcat
     6001 cgacaaaaac cctgccgtct gggcaaaatc atcactccct ttctgccacg ccacgctgcc
     6061 gcgcagggac actcgctgac tgatattgcc cgtgactccc acttttattt caccccgtcg
     6121 cttcaccgca tcgtcactga tagtgctgcc atcttcttca atttccgtcg catggggatc
     6181 gtgataataa ttcagatcta atgttggtgt gacaccaacc gcggtacgcc attcgctgtg
     6241 taaacccagc cgcgtctgaa tatcatcccc ctgcgattgt gacacgcgcg catggttagc
     6301 ggcggtaaaa tcatcctgct gcacgccctg ataaatcacc tgcgcctgcg gttcaatcac
     6361 cacaccacgc cccggtaacc actgataccc ggcttccagc gaggcgataa tccccgacga
     6421 gtggtaatga tctgtgccat cgtcttgttc ggaaacatca ttgttaaacc acgcgtattg
     6481 cagccagcta tccagccagg ccccctgctt ctgattaccg tgctgatacc agcttgaggt
     6541 cagcccaacg gcataaccgt ggttctggtt atcggcgcga gttccggtca tattcgagcg
     6601 gctctcgccc tgattatcac tgtagccacc aaccgcccca agcatccact cgccatcatc
     6661 gccccagcgc ccgctaaaca ggtcaccgct aagctgcacc gtagaagtgt cttcatgctg
     6721 agccagttgc cctgccgccg tgtaatgata acgtccgccg ataacacgta aattcagcgt
     6781 ctgaccattg ccacctgcat gatcgcgtcg ctccatcata aacgcctgat ttgccgcccg
     6841 cagattattg agataaccgc caactttggc attcagcacc ggctggtaag cggggattgg
     6901 atcgggtgtt gggtcaggat cgggtgttgg gtcagggtct ggatcaggtg gcaatggtgg
     6961 cgttacttct tgcgatcgca gataccagtc gttgttatct tccaccagcg tgtagtcata
     7021 tgctcccata ttgacgtagc cgctgcctgc cagactgaac ttcgcattgt tttgaaactg
     7081 cgtgggatcg gctgcgaaat caaccacttt aatgcctgtc gatgtcggct caccaatccc
     7141 tgtaatggag ttaaccacca cagttgtgtt gccagcagta ttaccgttca acaccaattg
     7201 atcgcttacc gagtcatcgc cgtttaattc gctatcgagc agtagcgtac cgccgccggt
     7261 ataatcaccg tttaccgtca gcgtatcgcc agcgacgcca ttttgcaggc taaccgcacc
     7321 ggacgtattg gtcagattgc cattaaccgt cacgtcggaa agataggtca ggctgtcagt
     7381 ttcaaggttg gtattaagct cacctgtcaa cgtcacgcca tcggtggcat tcaccagcgt
     7441 cccatcgccg ttaaacagtg aggcattaag ggcgacagaa gtatcctgcc cggtcaggcg
     7501 caaaaccgta ccatcgctga tgtcgatagt gccgctggaa gtagcatcaa tagattgaat
     7561 atcctgatcc gcgccagtaa cgaacgtcgc cccatcaccg acccacagtg acgaagcata
     7621 aggaaggata tccgcgacat cgcctttcag ggtgccttct tcgacacgta ccgccgactg
     7681 cgttgtaccg ctggcggtca gctccagcgt acccgccccc gttttagtca atgtgctacc
     7741 ctcatcctga tgctgcccgc tgctgtcagc catcagtgcg ccccactgcg tgtctacccc
     7801 cgcatcaact gccacttcac cgtcggcgcg catttcaata tcacgcccat gctcagtcgt
     7861 cgtagagcga gtatcgacat tggcagtaat ctccatcacg ctttcttgtt gcttatcggt
     7921 aaagataacc tggcggttat agtgggtatc tccaagctgt gagttgtcgc tcacctccag
     7981 cataccggag gcgatttgtg ttgtgcccaa ataactatta ttgtttgcca gacttacctg
     8041 cccgtcgccc gcatcgacca ccacgtagac gccatctttc ccgtcaccgc cgatatgcat
     8101 atcgtttcca cctgcaagat ctaccacatc ctgactgccg ataaccgttc cgctgctgcc
     8161 gctgacttca aggccatcgt tgtatgatgt gccactctgc caggtggaga aatcagagag
     8221 atcgagcaca ccgccgttta acacgatcga ctgcggatcg tcctggagag cggtaagatc
     8281 tgccgcgtcg ccttccagcg aaagcaccgc accatcatca acgactatat cgccggttag
     8341 cgccatcgac tgcgcccccg acagcacgta gctgccgttt tgcgcaatgg tgagctgacc
     8401 agcaccttcg atggtgccag caaaactgcc ttgattaaca gtaacattgc caccagcatc
     8461 gatatttaaa gtgccattct gaaagcccgt caatgagtgc acaaaggttt gttgggtgga
     8521 gccaacgttt agttctgcct gattctggta cttatcaata ctccctatcg ccagaccgta
     8581 gcagtcttgc ggatcgtcct ggcaatgcgt atcgccgaca ttcatcaggg agttgctgcg
     8641 acccagggta acttcaccgt tttcaatctg catctcacca gtaaagtcat tgttatctgc
     8701 attaagtacc agatcgccgg aacctgtttt ggtgatcaac ccggtaccag caatagagtc
     8761 aacagctccg tcattctctg tattgccaat aaccagcgtt tttccgtcgg caatatcaaa
     8821 ggtaacttca cttaagccga gatacataaa gccacccgcc gcagtggaag gaccatctcc
     8881 atagcctgct gcgctgttgt tctcatccac taacacgccg ccgttctggc tgtagctgtc
     8941 atcaacagaa atatcaataa gatagggagc cgtcgcgcta ttggtatata tcgccccgcc
     9001 ataaccttcg gcagtgttat ttgtaaaggc ggtgttattt attatcgtat aacctgaagg
     9061 atgcttgctg tcgctattat tatcggtaac atcgattgcc ccgccatcgc catcactgta
     9121 acttgttgat gtatatgcct ggttgttatc aaaaataaca tcacttaaat aaacgtcatt
     9181 attaatggta taaattgcgc cacctttgcc atcattagcg atgttattgc gaaacatggc
     9241 gttagtgaca cgtaaatcga cgacaccagt atcattagta ccagaagaat agattgcgcc
     9301 accatagccg cctgcgacgt taccagaaaa aataacatca gtaagattca gcgttgagtt
     9361 ttctttagca aatatggccc cgccgttatt atattctcct gtaacggtgt tattggcaaa
     9421 cagagtcatt ccagtttcat tttctggtaa tagtgaaaat tccgctcctt gttgcaaaaa
     9481 tacggctcct ccgctggcgt tattggtcat atccgaaaaa accagccatt gcccatcggc
     9541 gacactccag tcctgcgtaa tgcctgaaag gctttgcctg ctggcgtgac aactcgcttt
     9601 gatatcatat ccctggcata aatcaatggc cgccgcgaaa ccgttagcag agaaaagaga
     9661 tgcaatcatt gacgggagta gagataaata ctccttgcgt agaaagataa tccgcatatt
     9721 catctaacca tcattttata taagacggca tatttaaacg cattatacaa tatgggttat
     9781 ctttcttttg taaattgtcc aacccgatgc aatggcta
//
LOCUS       DAZGLJ010000169         9787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000169 DAZGLJ010000000
VERSION     DAZGLJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.169"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACHK49_003648"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACHK49_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO9264225.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="ACHK49_003649"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="ACHK49_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO9264226.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHK49_003650"
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHK49_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO9264227.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5797..6906)
                     /gene="mdtE"
                     /locus_tag="ACHK49_003651"
     CDS             complement(5797..6906)
                     /gene="mdtE"
                     /locus_tag="ACHK49_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO9264228.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7014..7292
                     /locus_tag="ACHK49_003652"
     CDS             7014..7292
                     /locus_tag="ACHK49_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264229.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="ACHK49_003653"
     CDS             complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="ACHK49_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO9264230.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="ACHK49_003654"
     CDS             complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="ACHK49_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO9264231.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9446..9778
                     /gene="hdeA"
                     /locus_tag="ACHK49_003655"
     CDS             9446..9778
                     /gene="hdeA"
                     /locus_tag="ACHK49_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO9264232.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VEESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
BASE COUNT         2677 a         2234 c         2238 g         2638 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctatt ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4441 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5401 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atacattctg
     6061 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     6121 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     6181 gaatttcagc gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     6241 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgtaagaa
     6301 atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6361 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6421 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6481 cgctttggcg acggtgacat tggcttctgc ttcattcaac tgggtgcgcg cggtatcgta
     6541 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6601 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6661 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6721 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6781 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6841 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
     6901 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6961 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
     7021 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     7081 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     7141 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat aaagtcgttt
     7201 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     7261 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     7321 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     7381 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     7441 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     7501 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     7561 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     7621 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     7681 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     7741 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     7801 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     7861 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     7921 tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     7981 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcaattt gtaaatatca
     8041 aacaaaaagt taacgtttgc ggattactgg ccaagaataa gttatcaata accagtttgt
     8101 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     8161 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     8221 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     8281 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     8341 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     8401 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     8461 aaaaaaatta gatgaaattc agtaggttga aatgatcact agcaggtaat tatttcaatg
     8521 atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg gatcatagtc
     8581 acatcaagtg actatgatcc tggtgacaac cgggataatt attgctgctt aacgaacaaa
     8641 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     8701 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     8761 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     8821 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     8881 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     8941 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     9001 agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     9061 acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc aataaactgg
     9121 attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt tgccttatct
     9181 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     9241 ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg cagtatctga
     9301 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tccatcaaca
     9361 tgacatatac agaaaaccag gttataacct cagtgtcaaa attgattcgt gacggctctt
     9421 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     9481 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     9541 accggtcaac tcctggacct gtgaagattt cctggctgtg gaggaatcct tccagccaac
     9601 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     9661 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     9721 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     9781 ccgggaa
//
LOCUS       DAZGLJ010000170         9748 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000170 DAZGLJ010000000
VERSION     DAZGLJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.170"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(83..2209)
                     /locus_tag="ACHK49_003656"
     CDS             complement(83..2209)
                     /locus_tag="ACHK49_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO9264233.1"
                     /translation="MAETLEKKHERIMLRFDRAYSPQQEVREKCIEATRFARVPGGQW
                     EGATAAGTKLDEQFEKYPKFEINKVATELNRIIAEYRNNRITVKFRPGDREASEELAN
                     KLNGLFRADYEETDGGEACDNAFDDAATGGFGCFRLTSMLVNEYDLMDDRQRIAIEPI
                     YDPSRSVWFDPDAKKYDKSDALWAFCMYSLSPEKYEAEYGKKPPASLDVTSMTSWEYD
                     WFDEDVIYIAKYYEVRKESVGVISYRHPITGEIATYDSDQVEDIEDELAIAGFQEVAR
                     RSVKRRRVYVSVVDGDGFLEKPRRIPGEHIPLIPVYGKRWFIDDIERVEGHIAKAMDP
                     QRLYNLQVSMLADTAAQDPGQIPIVGMEQIRGLEKHWEARNKKRPAFLPLREVRDKSG
                     NIIAGATPAGYTQPAVMNQALAALLQQTSADIQEVTGGSQAMQQMPSNIAQETVNNLM
                     NRADMASFIYLDNMAKSLKRAGEVWLSMAREVYGSEREVRIVNEDGSDDIAVLSAQVV
                     DRQTGAVVALNDLSVGRYDVTVDVGPSYTARRDATVSVLTNVLSSMLPTDPMRPAIQG
                     IILDNIDGEGLDDFKEYNRNQLLISGIAKPRNEKEQQIVQQAQMAAQSQPNPEMVLAQ
                     AQMVAAQAEAQKATNETAQTQIKAFTAQQDAMESQANTVYKLAQARNIDDKAVMEAIR
                     LLKDVAESQQQQFQSPPQSPADLMPS"
     gene            complement(2212..3624)
                     /locus_tag="ACHK49_003657"
     CDS             complement(2212..3624)
                     /locus_tag="ACHK49_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFO9264234.1"
                     /translation="MTSINPIFEPFIEAHRYKVAKGGRGSGKSWAIARLLVEAARRQP
                     VRILCARELQNSISDSVIRLLEDTIEREGYSAEFEIQRSMIRHLGTNAEFMFYGIKNN
                     PTKIKSLEGIDICWVEEAEAVTKESWDILIPTIRKPFSEIWVSFNPKNILDDTYQRFV
                     VNPPDDICLLTVNYTDNPHFPEVLRLEMEECKRRNPTLYRHIWLGEPVSASDMAIIKR
                     EWLEAATDAHTKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLMDINE
                     GADWATSLAIEDGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDGP
                     YQAGAWADEVVQGDNVRTIGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLS
                     FDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCM
                     HCPALVREETEIYVPSSSGW"
     gene            complement(3621..4046)
                     /locus_tag="ACHK49_003658"
     CDS             complement(3621..4046)
                     /locus_tag="ACHK49_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin carboxyl-hydrolase"
                     /protein_id="HFO9264235.1"
                     /translation="MATEKKKGGRPSDYMPEVANDICALLSSGESLRKVCERPGMPSK
                     TSVFRWLAEHQEFRDQYAKATETRADSIFEEIFEIADDVIPDAAEVAKARLRVDTRKW
                     ALARMNPRKYGDKVTNELVGKDGGAIQIETSPMSTLFGK"
     gene            complement(4126..4368)
                     /locus_tag="ACHK49_003659"
     CDS             complement(4126..4368)
                     /locus_tag="ACHK49_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HFO9264236.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITTRTIEAVRKSKEALDLFTTGA"
     gene            complement(4472..4843)
                     /locus_tag="ACHK49_003660"
     CDS             complement(4472..4843)
                     /locus_tag="ACHK49_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HFO9264237.1"
                     /translation="MNDQQIEKEIVEKGKTAPRVTPDHIEGIIAQEAYFTAEDGAFGK
                     AIKAKHTGGEVNYQSHESLSLLTFCVLVLRNGFTVTGESACASQENFDAEIGRKIARQ
                     NAVNKIWMLEGYLLKQKLSEQ"
     gene            complement(5127..5645)
                     /locus_tag="ACHK49_003661"
     CDS             complement(5127..5645)
                     /locus_tag="ACHK49_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO9264238.1"
                     /translation="MNHQLANLDFRDMVVVSGDRVITTSRKVAAYFDKQHHHIIQKIE
                     KLDCSDEFLTSNFSRVTYEHKGNQYVEYEISKDGAMYIIMSFTGKKAAAIKEAFIKAF
                     NWMRDRLMEMAHSYQREHNELMLEFMKEKDVASMSGRLLNRWGRIKKPQLIARIKRLE
                     QQAQISIPGLPK"
     gene            complement(5851..6288)
                     /locus_tag="ACHK49_003662"
     CDS             complement(5851..6288)
                     /locus_tag="ACHK49_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO9264239.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            complement(6285..6761)
                     /locus_tag="ACHK49_003663"
     CDS             complement(6285..6761)
                     /locus_tag="ACHK49_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO9264240.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            complement(6745..7068)
                     /locus_tag="ACHK49_003664"
     CDS             complement(6745..7068)
                     /locus_tag="ACHK49_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFO9264241.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            complement(8141..8764)
                     /locus_tag="ACHK49_003665"
     CDS             complement(8141..8764)
                     /locus_tag="ACHK49_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO9264242.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            complement(8761..9426)
                     /locus_tag="ACHK49_003666"
     CDS             complement(8761..9426)
                     /locus_tag="ACHK49_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO9264243.1"
                     /translation="MRYYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKAGELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQLIW
                     NRERISNSQDGIVKEIKGADMFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIHVQG
                     EGA"
     gene            complement(9404..9610)
                     /locus_tag="ACHK49_003667"
     CDS             complement(9404..9610)
                     /locus_tag="ACHK49_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO9264244.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            complement(9607..>9748)
                     /locus_tag="ACHK49_003668"
     CDS             complement(9607..>9748)
                     /locus_tag="ACHK49_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO9264245.1"
                     /translation="RIGQEAVDEIESNHSRHRWTIEECKAIKAEYQQKLKDLRNSRSE
                     AA"
BASE COUNT         2213 a         2505 c         2221 g         2809 t
ORIGIN      
        1 ttcttctgca ttttcctgtg tttcagacag gtcaataacc tgaccgtcga tgatcagttc
       61 gttttccatt gattactcct ggttaactcg gcattaagtc tgccggtgac tgtggtggtg
      121 actggaattg ctgttgttgt gactcggcga catctttcag aaggcgtatt gcctccatca
      181 ctgctttgtc atcgatgttt ctggcttgag ccagtttata gacagtgttt gcctgactct
      241 ccatcgcatc ctgctgggca gtaaatgctt tgatttgagt ttgagcagtt tcgttagttg
      301 ctttttgcgc ttctgcctgc gctgctacca tttgcgcctg agcgagaacc atttcaggat
      361 ttggctggct ttgtgctgcc atttgcgcct gttgaacaat ctgctgctct ttctcattgc
      421 gtggttttgc aataccagat atcagcagtt ggtttcggtt gtactctttg aagtcatcaa
      481 ggccttcgcc atcgatattg tccagaataa taccctgaat tgccgggcgc attgggtctg
      541 ttggaagcat agagctaagg acatttgtca gtacagaaac cgttgcatca cgtcgtgctg
      601 tgtagcttgg tccaacatca accgtcacat cgtatcgacc gacagaaagg tcatttaacg
      661 caacaacagc ccctgtttgc ctgtcaacaa cctgtgcgct caggaccgcg atatcatcac
      721 ttccatcttc gttaacgatg cgcacttcac gctctgaacc gtacacttca cgcgccattg
      781 acagccatac ttcaccagcg cgtttaagac ttttcgccat attgtccaga tagataaacg
      841 aagccatatc tgctctgttc atcaagttgt taaccgtttc ctgagcaata ttacttggca
      901 tctgctgcat ggcctgactg ccgcctgtaa cctcctgaat atcagcactg gtttgctgta
      961 gtaatgcagc caatgcctga ttcataaccg caggctgtgt atatcctgcc ggggtagctc
     1021 cagcgataat gttgccagat ttatctctca cttcgcgcaa cggcaagaac gctgggcgtt
     1081 tcttgttgcg agcctcccag tgcttctcaa gtccacgaat ttgctccatg ccaactatag
     1141 ggatctgacc ggggtcttgc gctgcagtat cagccagcat tgatacctga aggttataca
     1201 aacgctgtgg atccattgct tttgcaatat gtccttcgac acgctcaatg tcatcaatga
     1261 accagcgttt tccataaacc gggatgaggg ggatatgctc accaggaata cgtcgaggtt
     1321 tctcaaggaa accatcacca tccactacgg atacatacac acgacggcgc ttcactgagc
     1381 gccttgccac ttcctgaaat ccagctattg ccagttcatc ttcaatatct tcaacctgat
     1441 cactgtcgta tgttgcaatc tctccagtga ttggatgtcg ataactgatg acgccaacag
     1501 actctttacg aacttcgtaa tacttcgcta tgtaaataac atcttcatca aaccagtcat
     1561 attcccaact ggtcatagac gttacatcca gagaagcagg aggtttcttt ccgtattcag
     1621 cctcatattt ttcaggtgac aacgaataca tgcagaacgc ccacaacgcg tcagatttgt
     1681 cgtacttctt agcgtcaggg tcaaaccaca cagagcgcga cgggtcgtat attggttcaa
     1741 tagcaatacg ctgacgatcg tccatgaggt cgtattcatt gaccagcatc gacgtcaaac
     1801 ggaagcaacc gaaaccacca gtagcagcgt cgtcaaatgc attatcgcaa gcctcaccgc
     1861 catcggtttc ttcgtagtca gcacggaaca gaccatttaa tttattggct aactcttcgc
     1921 ttgcctctct gtcaccagga cgaaacttaa cagtgattct gttattgcgg tattctgcaa
     1981 tgatgcggtt aagttcagtt gctaccttat tgatttcaaa cttaggatac ttctcgaact
     2041 gctcatcaag cttagttcca gccgccgttg ctccttccca ttgacctcct gggacacgag
     2101 caaacctcgt agcttcaatg cacttttcgc gcacttcctg ctgtggagaa taggcgcggt
     2161 caaacctgag catgatccgc tcatgttttt tctctaatgt ctctgccatg tttaccaacc
     2221 ggaggatgag ggaacgtata tttctgtttc ttcgcggacc aatgccgggc aatgcataca
     2281 catcatcagc gcatcagcca ggttaggaga tgggataccg agcttctgct tcatttcgac
     2341 cttagtcata agctccagct tcccgttatt attgaatttg cgctgaatct gcgtcagttc
     2401 tgcaaacagc ttctccagca tcttctcgcc tatcgcttct ttgtcgaagc tcagcatgtc
     2461 gtcggggtct gcatactcac cgtggacaac cgcccgatat gtcagataca gcctgtcagc
     2521 cagcgcgtaa tagaattgcg ctcgcttatt gcggaataca tcgccaatag tgcgaacgtt
     2581 gtcgccctgt acgacttcat cagcccatgc tccggcctga taaggcccat cttcatcgaa
     2641 tggcgattcg ctgcccttga acatcgtggc ggtaattttc ttgccggaga atgcttccgt
     2701 tgtctgtctg cgtaggcccg caccgacgcc atcaccgtcc cacaggtaat ggtcagcgcc
     2761 gtcttcaatc gccagcgaag tagcccagtc agcaccctcg ttgatgtcca tcagcagacc
     2821 ttcggcaatg cgcttaacta ccgaaccgtg acgcgatgca taacctttag catctggccc
     2881 tgtatctgat gggtcatgcg cagaaacaac cgcacctttc gctttccacc cgagtttcgt
     2941 gtgcgcatcg gttgcggctt caagccattc acgtttgatg attgccatat cacttgcgct
     3001 taccggctca ccaagccaga tgtgacgata cagtgtcgga tttctgcgtt tacactcttc
     3061 catctccaga cggaggactt caggaaaatg cgggttgtcg gtgtagttca ccgtcagcag
     3121 gcaaatatca tcgggaggat ttacgacgaa tcgctgatag gtatcgtcga ggatgttttt
     3181 cgggttgaaa cttacccata tttcagagaa cggcttacgg atggttggaa tcaggatatc
     3241 ccacgattcc ttcgttaccg cttccgcttc ttccacccag cagatatcaa tgccttcgag
     3301 cgatttaatc ttcgtcgggt tgtttttgat gccgtagaac atgaattcag cattcgttcc
     3361 gagatgacga atcattgaac gctgaatttc aaactcagcc gaataccctt cacgctcgat
     3421 ggtgtcttca agcaacctga ttaccgaatc gctgatactg ttttgcagct cacgagcgca
     3481 aagaatacgc acaggctgcc gacgcgccgc ttcaacaaga agcctcgcaa ttgcccatga
     3541 cttaccgcta cctcgaccgc ctttggcgac tttgtagcga tgagcctcaa tgaacggttc
     3601 aaagatagga ttaatcgagg tcattttccg aatagagtgc tcatcggtga tgtttcaatc
     3661 tggattgcgc cgccgtcttt gccgacaagc tcgttagtta ccttgtcgcc atacttacgg
     3721 ggattcattc gggccagcgc ccatttgcgg gtatcaacgc gaagtcttgc ctttgccacc
     3781 tcggcggcat cagggattac gtcgtcagca atttcgaata tctcttcgaa aatagagtcg
     3841 gcccgagtct ctgttgcctt cgcgtactgg tcacgaaact cctgatgttc agccagccag
     3901 cgaaaaactg atgttttgct cggcattcct gggcgttcgc aaactttgcg cagactctca
     3961 ccggaggaaa gcaatgcgca aatgtcatta gccacctccg gcatataatc agaggggcga
     4021 ccacctttct ttttctcagt cgccatattg attatttccc ttctgcttgc ttatcccatt
     4081 catcgcggaa tttggatggg ttgtcgaaac cttgagttgc catgtttacg ctccggtagt
     4141 gaacaggtct aacgcttcct tcgatttacg caccgcttcg attgtgcggg tcgtgatatc
     4201 tgaattagcg ccacctgact ggaagtgaat tttgaatagc tcaagcttca gctcgtcagt
     4261 gccaatgaac tgaaatgctt cctccgcggc tgcgttctgg ttcatgacca gtttgtaaat
     4321 ctctaactgg aatttctgtt cttcagtcat gggaataatc tctgccattg ttggctccgt
     4381 ttatccgtta aaagggatat cagttaagtt atcccgtgta gggtataagc cattatcaaa
     4441 gccactcagt aaggaatggc ttttgtaata actactgttc gcttagcttc tgcttcagca
     4501 agtaaccttc gagcatccag attttgttta cagcattctg ccgggcaatc ttccgaccaa
     4561 tttctgcatc aaaattttcc tgacttgcac aggcactctc tccggtgacg gtgaagccgt
     4621 tgcgcagcac caggacgcag aacgtcagca gagaaagtga ttcgtgcgac tggtagttta
     4681 cctctccgcc agtatgtttc gcttttatgg ctttgccaaa ggcaccatcc tctgctgtga
     4741 aatatgcctc ctgagcaata attccttcga tatggtctgg cgtaactcgc ggtgccgttt
     4801 tgcctttctc aacgatttct ttttcgattt gctggtcgtt cataattatg accctgtaga
     4861 gtggttgctt gattaggatg tctttccatc agttcgccac cacaaagaat cttttttgcc
     4921 ataaggctgg aggttcatct ttcagtggct gccagtgtta tttccccact tactggcttg
     4981 ggttgtttcg cggtactgcc gtaactggtg gtgcacagat ttagttaaat ctgttctcgc
     5041 ctgaactatc ttttacatac ccggattgtg gggatgtaaa tcacggtttc attatcaagc
     5101 ccacccgtag atgggctttg gaatggtcac tttggcagtc cggggatcga tatttgcgcc
     5161 tgctgctcaa gccttttgat tcttgctatg agttgcggtt ttttgatcct gccccagcgg
     5221 ttcagcaagc gtcctgacat actggcaaca tccttttcct tcatgaactc cagcattaac
     5281 tcgttgtgct ctctttggta tgagtgagcc atctccatca gcctgtcacg catccaatta
     5341 aatgctttga taaacgcctc tttgatggcg gcagcttttt tgccggtaaa cgacatgatg
     5401 atgtacatcg caccgtcttt ggaaatttca tattcaacat actgattacc cttgtgttca
     5461 taggtaaccc gcgaaaagtt gctggttaga aattcatccg aacagtctag cttttcgatt
     5521 ttctgaatga tgtggtgatg ctgcttgtcg aagtaagctg ctaccttgcg ggaggttgtg
     5581 atcacgcgat caccagaaac aaccaccatg tcccggaaat cgagattagc caattgatga
     5641 ttcatagcgt ctttaccttt tagaaagtga gcctgtctca cagaaaagcc gccccgagat
     5701 ggtcgccacc atatacggca gttctcaggc tcaactttct gaaaggctcg ggtgatgtaa
     5761 tatgcgcgtg agatgcgctg tgaaattcag atgtaaaaaa agccccgcat cgcgaggctc
     5821 attaaatgga ctttgtgatt tgcaaaaaaa ttatttcagg cactgagtcc tgatgtactc
     5881 ctgcaggtag ttaacctgcg cggttatcct gtcgattcca cttcggagac ggtaataatt
     5941 gagttcagca tctgctgtaa gtcttgggct ttctccatcg cccatgctgc tggctccggt
     6001 cgttgacttt gcacaggtgg cggcgacttg caggcgctta cgaccagcag aaacatcagc
     6061 acgaaggctt tcgatagtag cgttagcatc agcaagctcc tttgtgtatc tggcgtcgag
     6121 ttctgctaca tcacgttgac gcttctgcat atcagcgatg atggatgtgg ctttatcgcg
     6181 ctgctctttg taggtcatgg cgttatcacg gtaatgatta acagcccatg acaggcagac
     6241 gatgatgcag ataaccagag cggagataat cgcggtgact ctgctcatac ctcaatctct
     6301 ctgaccgttc cgcccgcttc tttgaatttt gcaatcaggc tgtcagcctt atgctcgaac
     6361 tgaccataac cagcgcccgg cagtgaagcc cagatattgc tgcaacggtc gattgcctga
     6421 cgaatatcac cgcgatcaat catcggcaaa gcgccacgct ctttaatctg ctgcagcgca
     6481 acagcgtcct ggcttttggg agagaagtct ttcaggccaa gctgcttacg ataggcatcc
     6541 caccaacggg aaagaagctg gtaacgtccg gcggctgttg atttgagttt tgggtttagc
     6601 gtgacaagtt tgcgaggatg atcggagtaa tcagtgaata gctctccgcc aacaatgacg
     6661 tcataaccat gatttctggt tttctgccgt ccattatctg ttccctctga ccacgccagc
     6721 atatcgagga acgccttgcg ttgattattg atttccacca tcttctactc cggctttttt
     6781 agcagcgaag cgtttgataa gcgaaccaat cgagtcagta ccgatgtagc cgatgaacac
     6841 gctcgttata taagcgagat tgctacttag tccggcgaag tcgagaaggt cacgaatgaa
     6901 ccaggcgata atggcgcaca tcgttgcgtc gattactgtt tttgtaaacg caccgccatt
     6961 atatctgccg cgaaggtacg ccattgcaaa cgcaaggatt gccccgatgc cttgttcctt
     7021 tgccgcgaga atggcggcta acaggtcatg tttttctggc atcttcatgt cttaccccca
     7081 gaaggggatc tgttcaaatt aggaattatg gatatggtcg cttgaacaaa tccgggttac
     7141 ggttgatttg taacgggttt gttcgtgacc gcattcatga gcaaatcagg cgtggattgc
     7201 gccaacaata catgccgctc atatcacgaa gcccagccat tgatgctggg ttttcttttt
     7261 taaagcgtac tagacaaccg tatccacaga gtgtcagcaa tgagttggtt aggtctggtt
     7321 cttggtggaa gtacgcttta aaaaatgggc tgatggtgta gcccaaaata ctgagtgaat
     7381 ggtaaggatg aacaacggtt ttgctctggg tggatttggc tgtggtggcc ggcgctgatc
     7441 tccggcttgt atacaggcac cttgttcttc cgaagctctc ctgcgcgcat cagcctgcgc
     7501 attcaccaca ccggaaagag cactcagttg taccggccag ttgtgccact aagaaatgct
     7561 ttcgcagacc gttaagctct ttgccagttc tttaatgctc ttacctgttg tgtgcccatt
     7621 attaatcaca ccgggccagt gcgccgaatt tgtttacaag gagtcggaag accttgctga
     7681 cttacaggct attacgccgc catcagaaca acatcatcgt ttgcatttat ctttgtggtc
     7741 agtttctaaa aaaccgcaaa gtcgccaact ctgacgaaaa ctatcgttgt gctgccacaa
     7801 cgataagagc actcggtgca tttaagccaa gccccataag ggagaatgct cttacctgtt
     7861 acacagatat aaaaaatccc gaaaccgtta tgcaggctct aactattacc tgcgaactgt
     7921 ttcgggattg cattttgcag acctctcagc ctgcgatggt tggagttcca gacgatacgt
     7981 cgaagtgacc aactgggcgg aatcggtagt aagcgccgcc tctttttatc tcaataccac
     8041 aacgagcgaa ttaacccatc gttgggtcaa atttacccaa ctttattcaa taagtcaata
     8101 tcatgccgtt aatatgttgc catccgtggc aatcatgctg ctaacgtgtg accgcgttca
     8161 aaatgttgtc tgcgattgac tcttccttgt ggcattgcac caccagagcg tcatacagcg
     8221 gcttaacagt gcgtgaccag gtgggttgag taaggtttgg gattagcatc gttacagcgc
     8281 gatatgcggc acttgctggc atccttgaat agccgacgcc tttgcatctt ccgcactctt
     8341 tctcaacaac tctcccccac tgctctgttt ttgctatatc aaccgcacgg cctgtaccgt
     8401 gacaatctct gcatcttgcg cccggcgtcg cggcactacg gcaataatcc gcataagcga
     8461 atgttgcgag cacttgcagt acctttgcct tagtatttcc ttcaagcttt gccacgccac
     8521 ggtatttccc cgataccttg tgtgcaaatt gcatcagata gttgatagcc ttttgtttgt
     8581 cgttctggct gagttcatgc ttaccgcaga atgcagccat tccgaatccg gcttgtgatt
     8641 gcgccatccc catagcagcc atcacatcag taccggaaag agagtcagaa gccgtagccc
     8701 gtggtgagtc gctcatcatc gggctttttg gcgaatgaaa tttagctacg ctttcgagtc
     8761 tcatgcgcct tctccctgta catgaatcaa tgtgaggttt ccgcagaaca ctgcgccggt
     8821 atcgatatac atctggttgg caaacttgag tggtttcact gctggcgtat gaccaaagat
     8881 gaacatgtcc gctcctttga tttctttcac gatcccgtct tgtgagttgc tgattcgttc
     8941 gcggttccaa attaactgct gatgatcaac tggctttcca aactcgtatt cgtcacaagg
     9001 ataatcggcg tggcagatga catatttttt atctttgctc accagttcga tgattaacgg
     9061 aagttcacct gctttatggg caagagcttt agccagaatt tctttgtcgt aatcgagatt
     9121 aaagaaccag ccaccgccat taagcagcca gtgattgacg tttccacgct ctgataagcc
     9181 atcaatcatc atttgctcat ggtttccacg tacagctctg aaccagggga atgtgattaa
     9241 ttccagacat tcgacgttct ctgtaccgcg atcgaccaaa tcgccaaccg agataagcag
     9301 gtcttttttg gtgtcgaatc ctatcgtctc cagttttttc atcaggttcg tgtagcatcc
     9361 gtgcagatcg ccaactaccc aaatatttcg gtatttgctg ccatcaattc tttcgtaata
     9421 gcgcatctct ttcactccat ccgcgatgaa ccataagaac gtcgttgacg atggcgtgca
     9481 ttttcccgtc tttatcatca acgtattttc ttaccgtgcc gcgactacat ttcagtctgc
     9541 gtgctacttc tgtctggttt ccgtatgctt caacgagcat gtctggaatg gtttttactg
     9601 agaacgtcat gcggcctcac ttctgctatt tcgcaggtct ttgagtttct gttggtactc
     9661 tgccttgatc gccttgcact cttcgatagt ccagcgatgg cggctatggt ttgattcgat
     9721 ttcgtctact gcttcctgcc ctatgcgg
//
LOCUS       DAZGLJ010000171         9723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000171 DAZGLJ010000000
VERSION     DAZGLJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.171"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..543
                     /gene="ykgH"
                     /locus_tag="ACHK49_003669"
     CDS             <1..543
                     /gene="ykgH"
                     /locus_tag="ACHK49_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFO9264246.1"
                     /translation="INKDNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQR
                     ITTFFHTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDE
                     IQTPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIV
                     ISVMFCLVKKMVDEYQQNSG"
     gene            complement(789..1484)
                     /locus_tag="ACHK49_003670"
     CDS             complement(789..1484)
                     /locus_tag="ACHK49_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO9264247.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1477..2904)
                     /locus_tag="ACHK49_003671"
     CDS             complement(1477..2904)
                     /locus_tag="ACHK49_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO9264248.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2915..3634)
                     /locus_tag="ACHK49_003672"
     CDS             complement(2915..3634)
                     /locus_tag="ACHK49_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO9264249.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4161..5015)
                     /gene="rclR"
                     /locus_tag="ACHK49_003673"
     CDS             complement(4161..5015)
                     /gene="rclR"
                     /locus_tag="ACHK49_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO9264250.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5241..6566
                     /gene="rclA"
                     /locus_tag="ACHK49_003674"
     CDS             5241..6566
                     /gene="rclA"
                     /locus_tag="ACHK49_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO9264251.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEANLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            6675..6911
                     /gene="rclB"
                     /locus_tag="ACHK49_003675"
     CDS             6675..6911
                     /gene="rclB"
                     /locus_tag="ACHK49_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO9264252.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6923..7516
                     /gene="rclC"
                     /locus_tag="ACHK49_003676"
     CDS             6923..7516
                     /gene="rclC"
                     /locus_tag="ACHK49_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO9264253.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(7676..8545)
                     /locus_tag="ACHK49_003677"
     CDS             complement(7676..8545)
                     /locus_tag="ACHK49_003677"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO9264254.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            8794..9651
                     /locus_tag="ACHK49_003678"
     CDS             8794..9651
                     /locus_tag="ACHK49_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9264255.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
BASE COUNT         2705 a         2312 c         2072 g         2634 t
ORIGIN      
        1 atcaataaag ataatataaa agtgagttac agcctaaaaa taaaccagac aacaccaggc
       61 atacttgtta actgtgatag caataataac tttgcctgtc agaccacaat gactgaagat
      121 gttatacagc gaattactac attttttcac accagcccag atgtcaagaa cagagaaata
      181 aagctggaat ggtcaggaga taagagagat ttaccaactg ctgaagcgga aatatctcgc
      241 gtgcaggcgt ctattatcaa atggtatgcg tcagaatacc ataatggcag gcaagttctc
      301 gatgagatac aaacaccttc agcaattaac agtgagcttt atacaaaaat gatatacctg
      361 accaggaact ggtcattgta tccgaacggt gatggctgtg taactataag ctcaccagaa
      421 ataaaaaata aataccctgc tgccatttgc ctggctctgg gattttttct aagcattgta
      481 atttctgtaa tgttttgcct tgtcaaaaaa atggtagatg aataccaaca aaactctggg
      541 tagtaataaa cagcattctg atagagagtg cctgtataaa gctgaagtga caatgaccgg
      601 gaagggaacc cgttttcagg aatggatgcc tgtctttttc attgtaaata tcctggcatt
      661 actaatgcca ggatatttac agaagagcag cataaccatg acggaacggg gttaagtcag
      721 gttgtcctta acgctatttt caacctcgta tgccttcttc aggtttatgt tcagatctca
      781 tatctctctc agcaatcctc aataatcaga tacaccgctt tcaccggacc atgaaccccg
      841 acgactttga taagctcaat atccgccgtt gaactagggc cgctaatgat gttaatgcag
      901 gacggcattc gttcaccggc ctgcgctttc tgatgcaatt tttctgcgag ttgcgctaca
      961 cgcggcaaga tagtgctttt acgcaggata aaaagagaag attccgggag caggctcaat
     1021 gaacgcccgc gcccggcggc ggaaaaaaga accacgcctc ccgattcggt taaaccatat
     1081 tcagcataca cgacacccac tttagcctgc tcggcctgcg agatattctc ggcacctttc
     1141 gccggatccc aaacaacggc attgcattcc tgctgcaaac gttcactaat ccccaattcc
     1201 tccagcctcg tgtcaccgct aatcacgacc gactgatctc ccagctcttt acacagacgt
     1261 attgcagctt ctgccgcctt cgcctcgctg gtcagctcac agcgcgtcaa cataacatcg
     1321 ctggcaaact gaataaacgc gtcacagcgc tgctgttggt taagttgggt aagccgctcg
     1381 ttagcatagt tgttaagcgg cgcatcttct gcttgcggtt caagtcgcag cgggcgaccc
     1441 agtgcctgag caacgttatt caaaaattcg cctcgattat ccattctttt tctcctgcgc
     1501 ctgatgtttc ttaaaccaac tacggaaact ctctccgtca gcttcaggaa gatcgcgtgc
     1561 ttccatccag tcgctaatcg cgccaaattt gagtggtgtt ttgccgccat tgataaacca
     1621 gcttgccgca tgagcgccgg ccatcatccc gactttccac aatcctggat gactattggc
     1681 ataagcgaac atttttatcg cccgttgctc tgcttttgcg gtgatacctt tttcagccat
     1741 cacccgacga tgacgcaaaa tcagttttga cagcggaata cgcaccggac acacgctgtc
     1801 acaagcagtg cataaagagc aggcgtaggg taaatcttta aaatctttat agccgccaag
     1861 tagcggagaa atcaccgcac caattggccc tggataaata gagccatatc catgaccgcc
     1921 aatatggcga tatgccggac aagtattcat acaagccccg cagcgaatac agcgcagcac
     1981 atcccgaaat tcagaggcca gcacctcaga acgcccgtta tcgacaataa ccagatgaaa
     2041 ctcttcagga ccatcaacgt gccccgcttc gcgcggtcct gtcagccagg tgttgtatcc
     2101 cgtcaaacgt gcaccaacgg cactgcgcgc cagcatggtg atcaatacat ctacctcggc
     2161 aaacgtgggg gcaatacgct ccattcccat cactgcaata tgcgttttag gcagcgtggt
     2221 acacattcgc gcattacctt cattggtcac caggcatacc gaacctgtct ctgccaccgc
     2281 gaaattacag ccggtaatac ctatttcagc actgaggaaa tcttcgcgga ttttttgccg
     2341 gatgaataag gtcatcgctt caggcgtttc cggcccctca tagcccagac gttcgtgtag
     2401 cactcgacgg atctgatggc gatctttatg aattgccggg accacaacat gagatggcgg
     2461 atcttgatcc agctggagaa tgtattcacc cagatcggtt tcaatcacct gaatgccagc
     2521 atcctgcaac acatgattga caccaatctc ttcggtcacc atcgatttag atttcaccac
     2581 cttccgggca tttttgcgtt gggcaacctg taaaatgtag cgagtggcgt cttctttagt
     2641 ttttgcaaaa tagacgtgac cgccgttttg cgtcactttt tctgagagct ggtacagata
     2701 agcgtcgaga ttacttagaa catgatcacg tatctgggcg gcccgatcgc gccactcctc
     2761 ccagtgcccc aattcatcga ccattttttg ccgatttgcc ccaatacgct gctgcgcgtt
     2821 tgccaccgct ttgcgcatga tcggatcttc aatttgctga cggatgcgtg tcttaaaatc
     2881 tgtattactg gttttgatcg acatctttat atcctcagcg gctcatcaac acttcagcaa
     2941 tatgcatcac tttgactttc tgcccttccc gttgtaatcg cccactgatg ttcagcaggc
     3001 aactcacgtc agcaccaatt aaatactcag ggcggacttc catcaggtgc acaacctttt
     3061 ctttcaccat ctcgccggat atttcggcca ttttgaccga gaacgtgccg ccaaatccgc
     3121 agcaggtatc ctgttcagca aaggtcaaca gctccagtcc acgcacattt ttcagcagcg
     3181 taagtggctc gtccttcact cccagcttac gggccaggct acaagatggg tgatacacag
     3241 ctctcccttg caaactggca cctacatcga ctacccctaa tttattaaca ataaaagagg
     3301 tgagatcctg catacgcgcg gcaacctttt cggcacgtga tgcccattca ggttcatccg
     3361 ccagatacgt cgggtaactt tttacggcat aggtgcaaga gccagccggt gaaataatgg
     3421 gatcgtcgtt atcctccagt gcggcgatca gatttttcat ccctggaatc gcttctttga
     3481 tataaccgct attgatcgca ggctgaccgc agcatccctg tttctccggg aaatttacgc
     3541 gacagccgag tttttccagt agcagcacgg agtctcgtgc cattcttgat ttcagggcgt
     3601 caccaataca ggtgacaaag aaattgacat tcacaactaa tactccatta cttcatgccc
     3661 atttatgccc gatgtttccc ccccaccggg ggatcaatca tcaataaata tggcgttctt
     3721 ttttaattgg ctagcaacat accacccctc atttgtatga caaatgataa tctttcatcc
     3781 aaacttcaaa tgcaataact aattttattt ctttcggcat cgctcacatt ttatggggaa
     3841 atcgacgttc atttttggca taagaaatcc atttaaaaag gctacatccc ttatacctat
     3901 tcagagctac ttattcaggg gttattgaga tatacactga tggtgtacaa aaaaatcacc
     3961 gacaataatc acgtatcaag taatagtcgg ggttttattt aattgaagca atttagtttg
     4021 catttttatt taaaaactga gctagagtcc atcagaaaaa aatatataaa gcccaaacca
     4081 ccgttggaaa tagaaatagt tgaaaggctc acaactcccg gaatgcggat tatctttgcc
     4141 ctgatttctg gcggttttat tcagggtgca agctgtctga ccctttcccg atattctccc
     4201 ggagtacaac caaactcgcg gacaaacgcc ttgtgaaaag atgattcact ggcatagcct
     4261 actgactcag cgatcaccac aacagggagc atttcccggg aaaacatctg ggccgctatt
     4321 tgtagacgca actttgttaa tacagccagc ggcgtggttc cggaaacatc acggaaaagc
     4381 tgggcaaaac ttgcccggga catgtgagcg atgctggcca gcgattcgac ggtccaggcg
     4441 tgtcctggca tttccagcat ttgctgtatt accgcaccaa gacgtggatg cagaagcaaa
     4501 ctgagaatgt ttttctcagt attaacctgt gcaatccact cacgcaccgc gagggtaaag
     4561 aatgtagcgc agatctggct acacagtgca tccactcccg gcatcgccga tctggattcc
     4621 tgttgtaaaa acgggatcgc ctccctcagc cagttatatt ccacgctgtg gttaaccggt
     4681 gctaaaaaca gcgtttccgg caaagacgtt aaaaaataac gcgccgaatg ttgcaaccga
     4741 agagtgccgc agacaataca ggtcgattca ttatcgacat gactcaggcg atgagctgaa
     4801 ttttgtggta gcaggaccac atttcccggg cgtaatgtaa aaatctctcc cgtcggcatt
     4861 tccagcttcg ccgctccttg cgttaacgca tgccaacgaa taaccgataa ttccccggca
     4921 ccatgtggaa gctgccagtc acttcctaac acgcaattct tatcgatcgt tccttgtgga
     4981 gcgttaagca tcaacaaacg gctaagggca tccatatcag actcctgagt ggtttttgat
     5041 tacaaacacc aacaatctta gtaaataact aatctaatac catcaaatgg ttaatcatga
     5101 tttttgtttt gtgattctga ttaagaaaaa gaaaatacca gacgataaga caaaatatca
     5161 agcgttgtga agaaatggta tttgctcttt tgcgtctata gtcatgatgt caaatgaacg
     5221 cgtttcgaca ggaaatcatc atgaataaat atcaggcagt gattattggt tttggcaagg
     5281 ctggaaaaac attagccgtc acgctggcaa aagcaggttg gcgagtggct ctcatcgaac
     5341 aatcaaatgc aatgtatggc gggacctgta ttaatatcgg ctgcatccca accaaaacat
     5401 tggttcatga cgcacagcag cacacagatt ttgtccgtgc catacagcgt aaaaatgaag
     5461 tggttaattt tttacgtaat aagaattttc ataatcttgc ggatatgccc aatatcgacg
     5521 tgatcgacgg ccaggcggag tttatcaata atcatagcct gcgtgttcat cggcctgagg
     5581 caaatctgga aattcatggc gagaaaattt ttattaatac cggtgcacaa accgtggttc
     5641 cgccaattcc tggaattacc accacgccag gagtatatga cagcaccgga ttacttaatc
     5701 taaaagaatt gcctgggcat ttaggtattt tgggcggcgg atatattggc gttgagttcg
     5761 cctctatgtt cgctaatttt ggcagcaagg taaccatttt agaagcagct tcgctgtttt
     5821 tgcctcggga agatcgggat attgctgata atatcgcgac gattttacgc gatcagggcg
     5881 tcgatattat cctcaatgcc catgtggagc gaatcagtca ccatgaaaat caagtgcaag
     5941 tgcatagcga gcacgcccaa ctggcggtgg atgcactgtt aatagcttcc ggtcgtcaac
     6001 cggctaccgc ttcgttacat ccagaaaatg ccggtatcgc agtaaacgag cgcggggcaa
     6061 ttgtcgttga caagcaatta cataccaccg cagacaatat ttgggcgatg ggagatgtta
     6121 ccggcgggct gcaatttact tatatttcac tggatgatta ccgcattgtg cgtgatgagt
     6181 tgctgggaga aggcagacgt agtactgatg atcggaaaaa tgtgccttat tccgtattca
     6241 tgacaccgcc actgtccagg gttggtatga cagaagaaca ggccagagag agtggtgctg
     6301 atattcaggt ggtgacattg cctgtagctg caattccgcg tgccagagtg atgaatgata
     6361 ctcgtggggt attaaaagcg attgttgata ataaaaccca acgtatatta ggggcatcac
     6421 tgctgtgtgt tgactcccac gagatgatca atatagtgaa aatggtgatg gatgccgggc
     6481 tgccttatag catattacgc gatcagatat ttactcatcc gtcgatgagc gaatcactca
     6541 atgatctatt ttcattagtc aaataaactc aaaatcagac gccagaacaa atattctggc
     6601 gtctcagaga aaagaatctt attaattccc tgttactcta taacttctta atcacttcat
     6661 tgatggtatt ttatatgttt aaaaaatctg ttttatttgc aacactatta tctggcgtta
     6721 tggcattttc caccaatgca gatgataaaa taattctgaa acatatcagc gtctcgtcag
     6781 tatcagcatc accgacagtt ctggaggata ccattgctga tatagccaga aaatataatg
     6841 cttcatcctg gaaagtcaca tcgatgcgaa ttgataataa ttcaaccgca acagcagtat
     6901 tgtataaata aggatgttca caatggaaaa atacctgcac ctgttaagtc ggggagataa
     6961 aattggcctg acattgattc gtctgagtat tgcaattgtt tttatgtgga ttgggttatt
     7021 aaagtttgtc ccttacgagg cagacagcat tacaccattc gtcgcaaaca gtccactaat
     7081 gtcgttcttt tatgaacacc cggaagacta taaacagtat ctgactcacg aaggcgaata
     7141 caaaccagaa gcaagggcat ggcaaacggc caataatacc tatggttttt ccaacggtct
     7201 tggcgtcgtg gaggtgatta ttgctctgct ggttttggct aatcctgtca atcgctggtt
     7261 aggtttattg ggaggactga tggcatttac cacgccgctg gtaacactct catttttaat
     7321 caccaccccg gaggcatggg tacccgcatt gggtgacgct catcatggtt tcccttattt
     7381 atccggtgct ggtcgcctgg tattgaaaga tactctgatg ctggcaggtg ctgtcatgat
     7441 aatggcagat tcggcgcggg aaattcttaa acaacgcagt aatgaatcca gttcaacgtt
     7501 aaaaactgaa tattgataat ccgaacactg tctgttgttc actttttcag gcgtcaggcc
     7561 ccctttctga tgatgaagcc tgacgccata ttaccggaga tatgacagag tgaagatcaa
     7621 tatcactccc ctgttaaaag attcttgtcg tctcaggtaa aatatatgat taatatcaat
     7681 catgtatttt tacagcaaga caaccacgaa caaattcagg attaaaatgt tttaccgact
     7741 ccccaacata acccaaatca agcgaactaa tttgtgccat ttctttctct gtcagtgaga
     7801 aatcccagat agcaaaattt tcttcgatac gctgttgatg tgtcgatttc ggaataacga
     7861 ccactccccg ttgaatattc caacgcagaa tgacttgaga aatcgatttt tgatgggcat
     7921 ctgcaatact ctgaagcaga ttattttcaa agggtttatg tcgtccacca cctaacggag
     7981 cccatgcttc aggctgcacg ttataatact tcatggttgc cagcgcctct ggttgggcaa
     8041 aatagggatg caactcgacc tggttgacca tcggtttaac tcttaccgtt tcacaaaagt
     8101 tcgccagaac atgaggatag aaattggaaa ccccaattgc ttttaatttg ccctcttcat
     8161 aggcatcttc gagtgcacgc cacgcactga aataatcgcg catagcctgg tgcaataaat
     8221 agaggtcgaa gtactctagt cccgattttt ttaatgatgc ttcaatacct gctgctgccg
     8281 tatcctgatt caacatatcc tgcacccaca atttagacgt tataaataac tcatctcggg
     8341 tacataaacc ttcagatata gcttcacgaa cggcttcacc gacggcatct tcattaccgt
     8401 ataccgctgc agtgtcgatg agtcgataac ctgtgcggat agcgttcaac actgactgtt
     8461 tgcacacgtt tttatcggta acctgaaaaa caccaaatcc catcatcggc attttcagat
     8521 tgttacttaa tacggaaaat tccactgtca gtccctccat taggcatgaa caatgagtct
     8581 acgttaaaac gtaacctcaa attagtacgt ggattttgat atcacttatg caaaaaattc
     8641 attaataatg caggactgaa acctctctat tttcggggac aacgaagcag acgctaccag
     8701 tgcttttgcc ttcgcccttg ctgtttttga tacacttagg gcccagggca taacgaaaat
     8761 gtgcgatatg acgaagaatc aatggagaat gtgatgatca ggcagaagat tctacagcag
     8821 ctcctggagt ggattgagtg caatcttgag caccctattt caatcgaaga tatcgcacag
     8881 aaatctggct acagcagacg caacatccag cttctgttcc gaaatttcat gcatgtgcct
     8941 ttgggagaat acattcgcaa acgaaggctt tgtcgtgccg ccattcttgt ccggctcacc
     9001 gcgaaatcta tgcttgatat tgcactctct ttgcattttg attcacagca gtcattcagc
     9061 cgcgaattta aaaagttatt cggctgctct ccccgtgtat accgccaccg tgattattgg
     9121 gatctcgcaa atatcttccc ttctttttta atacgtcaac agcaaaagac cgagtgtaga
     9181 ttagtcaact ttcctgagac acctattttt ggcaattcat ttaaatatga tattgaagtg
     9241 tcgaataaat caccggatga agaagtcaaa ctgcgacgtc atcatttagc cagatgtatg
     9301 aataatttta agacggatat ctatttcgtc tccacgtttg aaccgtcaac aaaatcggtc
     9361 gatttgctca cggttgaaac ttttgccggt acggtatgca aacaaaccga ttcagagatg
     9421 ccgaaagagt ggacaattaa ccgaggactg tatgcctctt tccgctatga aggtgagtgg
     9481 gagcactacc ctgaatgggc gcgaaacctc tatctgatgg aattacccgc cagggggtta
     9541 gccagagtga acggcagtga tattgagcgg ttttattaca atgaaaattt cgtcgaaatt
     9601 gatagcaata acattgtttg cgaaattttt atccccgttc gtccggttta gttttcgcta
     9661 tatctaaatg aatttataac gagactactg tcaggaaatt caataaataa tggcgttgaa
     9721 gat
//
LOCUS       DAZGLJ010000172         9622 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000172 DAZGLJ010000000
VERSION     DAZGLJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.172"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            280..930
                     /gene="csgD"
                     /locus_tag="ACHK49_003679"
     CDS             280..930
                     /gene="csgD"
                     /locus_tag="ACHK49_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO9264256.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            935..1324
                     /gene="csgE"
                     /locus_tag="ACHK49_003680"
     CDS             935..1324
                     /gene="csgE"
                     /locus_tag="ACHK49_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO9264257.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1349..1765
                     /gene="csgF"
                     /locus_tag="ACHK49_003681"
     CDS             1349..1765
                     /gene="csgF"
                     /locus_tag="ACHK49_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO9264258.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1792..2625
                     /gene="csgG"
                     /locus_tag="ACHK49_003682"
     CDS             1792..2625
                     /gene="csgG"
                     /locus_tag="ACHK49_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO9264259.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2689..3180)
                     /gene="ycdZ"
                     /locus_tag="ACHK49_003683"
     CDS             complement(2689..3180)
                     /gene="ycdZ"
                     /locus_tag="ACHK49_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO9264260.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGSFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3282..3836)
                     /gene="ycdY"
                     /locus_tag="ACHK49_003684"
     CDS             complement(3282..3836)
                     /gene="ycdY"
                     /locus_tag="ACHK49_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO9264261.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3860..4597)
                     /gene="ycdX"
                     /locus_tag="ACHK49_003685"
     CDS             complement(3860..4597)
                     /gene="ycdX"
                     /locus_tag="ACHK49_003685"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO9264262.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4652..5590)
                     /gene="ghrA"
                     /locus_tag="ACHK49_003686"
     CDS             complement(4652..5590)
                     /gene="ghrA"
                     /locus_tag="ACHK49_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO9264263.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGADVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5824..5911
                     /locus_tag="ACHK49_003687"
     tRNA            5824..5911
                     /locus_tag="ACHK49_003687"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5858..5860,aa:Ser,seq:gga)
     gene            complement(6061..6903)
                     /locus_tag="ACHK49_003688"
     CDS             complement(6061..6903)
                     /locus_tag="ACHK49_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO9264264.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6988..7185)
                     /locus_tag="ACHK49_003689"
     CDS             complement(6988..7185)
                     /locus_tag="ACHK49_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO9264265.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7197..7688)
                     /locus_tag="ACHK49_003690"
     CDS             complement(7197..7688)
                     /locus_tag="ACHK49_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO9264266.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7685..8059)
                     /locus_tag="ACHK49_003691"
     CDS             complement(7685..8059)
                     /locus_tag="ACHK49_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO9264267.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(8106..8480)
                     /locus_tag="ACHK49_003692"
     CDS             complement(8106..8480)
                     /locus_tag="ACHK49_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO9264268.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPALATTS"
     gene            complement(8643..8864)
                     /locus_tag="ACHK49_003693"
     CDS             complement(8643..8864)
                     /locus_tag="ACHK49_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO9264269.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8927..9403)
                     /locus_tag="ACHK49_003694"
     CDS             complement(8927..9403)
                     /locus_tag="ACHK49_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO9264270.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9419..>9622)
                     /locus_tag="ACHK49_003695"
     CDS             complement(9419..>9622)
                     /locus_tag="ACHK49_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9264271.1"
                     /translation="ETWVLFNAMNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTV
                     HYYRLRDYALQHPECSAIMRIID"
BASE COUNT         2471 a         2375 c         2350 g         2426 t
ORIGIN      
        1 aaaccaaatg tacaactttt ctatcatttc taaacttaat aaaaccttaa gattaacatt
       61 ttaatataac gagttacatt tagttacatg tttaacactt gatttaagat ttgtaatggc
      121 tagattgaaa tcagatgtaa tccattagtt ttatatttta cccatttagg gctgatttat
      181 tactacacac agcagtgcaa catctgtcag tacttctggt gcttctattt tagaggcagc
      241 tgtcaggtgt gcgatcaata aaaaaagcgg ggtttcatca tgtttaatga agtccatagt
      301 attcatggtc atacattatt gttgatcact aaaccgtctt tgcaagcgac agctctcttg
      361 cagcacctta aacaatcgct ggcaattaca ggaaaattac ataatattca acgttctctg
      421 gacgatatat cttcaggctc tattattctt ctggatatga tggaagcaga taaaaagctt
      481 atccattatt ggcaggatac tttgagcagg aaaaacaaca atatcaaaat attgttgcta
      541 aatacgcctg aagattaccc gtaccgcgac attgaaaact ggcctcatat caacggcgtt
      601 ttttatgcca tggaggatca agaacgtgtt gtcaatgggt tgcaaggcgt cctgcgcggc
      661 gaatgctact ttacgcaaaa gcttgccagt tacctgatta cacattctgg taactatcgt
      721 tataacagca cggaatcagc cctccttact catcgggaaa aagagatcct gaataagctg
      781 cgtatcggcg cgtctaataa cgagatcgct cgttcgttgt tcatcagcga aaatacggtt
      841 aaaacgcatc tttataatct tttcaagaag atagccgtaa aaaaccggac acaagcggtt
      901 tcctgggcaa acgataacct caggcgataa agccatgaaa cgttatttac gctggattgt
      961 ggcggcagaa tttctgttcg ccgcagggaa tcttcacgcc gttgaggtag aagtcccggg
     1021 attgttaact gaccatactg tttcatctat tggccatgat ttttaccgag cctttagtga
     1081 taaatgggaa agtgactata cgggtaactt aacgattaat gaaaggccca gtgcacgatg
     1141 gggaagctgg atcactataa cggtcaatca ggacgttatt ttccagactt ttttatttcc
     1201 gttgaaaaga gacttcgaga aaactgtcgt ctttgcactg attcaaactg aagaagcact
     1261 aaatcgtcgc cagataaatc aggcgttatt aagtacgggc gatttggcgc atgatgaatt
     1321 ctaaataaaa aattgttcgg aggctgcaat gcgtgtcaaa catgcagtag ttctactcat
     1381 gcttatttcg ccattaagtt gggctggaac catgactttc cagttccgta atccaaactt
     1441 tggtggtaac ccaaataatg gcgctttttt attaaatagc gctcaggccc aaaactctta
     1501 taaagatccg agctataacg atgactttgg tattgaaaca ccctcagcgt tagataactt
     1561 tactcaggcc atccagtcac aaattttagg tgggctactg tcgaatatta ataccggtaa
     1621 accgggccgc atggtgacca acgattatat tgttgatatt gctaaccgcg atggtcaatt
     1681 gcagttgaac gtgacagatc gtaaaaccgg acaaacctcg accatccagg tttcgggttt
     1741 acaaaataac tcaaccgatt tttaagcccc agcttcataa ggaaaataat catgcagcgc
     1801 ttatttcttt tggttgccgt catgttactg agcggatgct taaccgcccc gcctaaagaa
     1861 gccgccagac cgacattaat gcctcgagct cagagctaca aagatttgac ccatctgcca
     1921 gcgccgacgg gtaagatctt tgtttcggta tacaacattc aggacgaaac cgggcaattt
     1981 aaaccctacc cggcaagtaa cttctccact gctgttccgc aaagcgccac ggcaatgctg
     2041 gtcacggcat tgaaagattc tcgctggttt ataccgctgg agcgccaggg cttacaaaac
     2101 ctgcttaacg agcgcaagat tattcgtgcg gcacaagaaa acggcacggt tgccattaat
     2161 aaccgaatcc cgctgcaatc tttaacggcg gcaaatatta tggttgaagg ttcgattatc
     2221 ggttatgaaa gcaacgttaa atcgggcggt gttggggcaa gatattttgg gatcggtgca
     2281 gacacgcaat accagctcga tcagattgcc gtgaacctgc gcgtcgtcaa tgtgagtacc
     2341 ggtgagatcc tctcctcggt gaacaccagt aagacgatac tttcctatga agttcaggcc
     2401 ggggttttcc gctttattga ctaccagcgc ttgctggaag gggaagtggg ttacacctca
     2461 aacgaacccg ttatgctgtg tctgatgtcg gctatcgaaa caggggtcat tttcctgatt
     2521 aatgatggta tcgaccgtgg tctgtgggat ttgcagaaca aagcagaacg gcagaacgac
     2581 attctggtga aataccgcca tatgtcggtt ccaccggaat cctgacagat aaaaaagccg
     2641 gaattatcat ccggcatttt actgtttaag cgaaagcgtg agtgcccctc acgctttatt
     2701 tttgatttcc tgctcacggt gagccgtctt tgcactacgc gccgccagcc acaggccgct
     2761 gtttttcatt gcgtaaccaa ataccagccc aagcgccagc gaaggtaaca ccagtttcca
     2821 gtcaccctgc ccggcaaacg tcgcgcatgc gcctataaac gaccccggaa caaatgaaag
     2881 cagcagctgt ttggcctgaa tacacatcag aaacgcgaca ataccggtta tgacataacc
     2941 gagaatttcc agatgtggtg ccagtgcact accgtaaata atgaccatcg cccacaccac
     3001 gccactaagc agggttgcag cggagatcgc cagccctttc agcccacctt gcgggcaggc
     3061 aaagtaggcc gtacagccca ggaagcccgc ccagcttagt aagcccaggg aaacagccac
     3121 ccatccccag ataccggaga gaatgcctgt tgtgattgca atagagagaa gtatgttcat
     3181 gccgcgcatg atagcagaaa acgcggccat gaatgagatc gagcacacat tttatgcaac
     3241 aacaaattat ctgttgcagt tatgagtgac gatgatcaca tttactcttc agaatcttct
     3301 tccagctcgt cccacattgc actaatggca tcacgggtta gcggtgccat ggtgcgccag
     3361 aaaggcgtgg ttgcatgggc ctccactttg ccgaggaacg caccacacca gggtaacaga
     3421 tactcactga acagtgtttc cagtgcttcg ctctcatctt ccgttgactg atcttccagc
     3481 caggaagccg caagcagcaa tgtgccgatg tgatcggctg gcgtatccgc cagtggcatc
     3541 cctcgttcgg aaagaaaagc gcgcacttcc gcttccgttg cgtcctcaac ccatgcgcta
     3601 cgatatggcg gcacggcaca ttcatcgccg ataaacaacg cattgtaatc ggcagagact
     3661 tgcgccatat cacagctttt ctgtaaacgc gtcagtaact catcctgctc cagtggccag
     3721 ttcgcagcca gtttcccctc acgaatcagg gtaaacagcg gcaccagtaa aggatcttgc
     3781 ggttggcggt aatagagcga acccagcaca cgacagagga tagaaaactc gttcattaat
     3841 atatttccat tactagtaat taaaggtctg caaattccgc aatcggtgcc ataccgcgag
     3901 attcaaggaa gttcagtaag cggcgcggcg aaacattcaa aatgcgctct ggcggaaaat
     3961 ctaccgcgtc gaggatttta agacactctt caaattcccc catggtaaac gcggtgtgag
     4021 aatccgagcc taatgccacc caacctccag catcacgtac cgctgcggct accgcacgac
     4081 agttgtcttc actgccctta cgtgagtgta aaaatgaaga attattgatt tccagcgcca
     4141 cctgatgttt cgcggctgct tcagcaacgg ctttcacatc tatttcatat ttgggatttc
     4201 cgggatggct tattatatgc acattgccgc tggcgatagt ggcgatcatc gcttgtgtat
     4261 tggtcgcttt gtcatgtggt gcaaaaaccg gctcatgaaa accggcaata atcagatcca
     4321 gcgagtcaaa cattttaccg ctgcaatcaa tttcaccatc aacattttta atgttagctt
     4381 cgatgccgcg caggatccct accccatcaa ccactcgcgg ccagatacgc atgttaatga
     4441 agtgccagtg atgcggagca tcttccatat ccgggccatg atcggtgatc gcaaaaagtt
     4501 taatgccctt ttgtttggcc tgggcaatgt aatcactcaa tgtgctatat gcatgtgtgc
     4561 tggcaacggt atgcatatga aggtcgacgg gatacataac tctctcctgt attcaattcc
     4621 cgccaaggat agcaggaatc ctgatgcttt attagtagcc gcgtgcgcgg tcgacttgcc
     4681 cgcagaccct ctcccctttt tcgagctggg caatggtgcg agaaatgtac tccacagctt
     4741 cagcgggacg ggtaatcgcg gcgacatgtg gtgttatcgt cacgcgtgga tgttgccaga
     4801 gcggactttc aggcggtaag ggttcacgat taaaaacatc caacattgcg cctttaactt
     4861 tgccgctatc cagcgccgcg agcaggtcat cttccacaac atgaacacca cgcgccaggt
     4921 tgaggagata cgcgccatcc ggtaattttt cgagtaattg ttgattaata atgccgacgg
     4981 tttcaggggt attcggtaac aaattaatca atacccgaca ttggctcaga aatgcagaca
     5041 gttcttcccg tccggcaaag ctttgcacgc caggccacga tttacgggtt cgactccagc
     5101 aacgcagcgg aaagcgccag gtttgcagac tctgagcaac tttactgccc agtacgcctg
     5161 cgcccaaaat gccgatggta aaatcttccc gatgatattc aggcagcggt tgccaatgcg
     5221 aactattttg ctggatgcga taatcgtcaa aacgtcgaaa ccaatgcagc acctgactga
     5281 cagcatattc ctgcatttgc tcgcccatac cggtatcttc caggcgaaaa agtggaacag
     5341 aagggttcag catttcaggg tgtgcctgta gcttgctcaa aatagaatca acatcggccc
     5401 cgagtgcgaa caccgcttta agatcgcgcc ctgccagcat ttcaacagga ggatgccaga
     5461 ctaaagcata atcagcagag tcattatctc cgcttttcca cgctctgact cttgcctgag
     5521 gaatagcttt gcgcagtgcc tcaatccacc attgggtatc gaacgttggg tgataaaaga
     5581 tgatatccat actgactccc gaaaagcgtt gtgcgaaatt tattcgcact tatcgttatg
     5641 atctacaaag gccaccagca taacaaatcc gtggtcggtg gcaaaaaaag cagatttcgc
     5701 ttattaaaac cacacattga ttgaagtttg aataaacgcg cgattttttc aaaaagtttg
     5761 ttgacctcag gtcatgattt ccctaaatta gcgcccgttc cagcaagaca ggaacgacaa
     5821 tttggtgagg tgtccgagtg gctgaaggag cacgcctgga aagtgtgtat acggcaacgt
     5881 atcgggggtt cgaatccccc cctcaccgcc agattataag cgcatgttat tgtttaacat
     5941 gcgctttttt attttccctt ccagacagta tgccatcggg tatgccatct gaaaccggtt
     6001 tatatgcccc gcgatcatat cttatcggac aatttccgat ctttgagcat actggcaaag
     6061 ctatactgcc agcatatcag gatagtgttt tgcgataata tcattcagtc tgtcaaccag
     6121 ttctggtttt ctgaacgtga tatgcgcgga gcccttctta aagtatctga tgctgaacat
     6181 ctcatcttca tagctctctt taccctgaac agactgaatg tgatcatcca gacgaagggt
     6241 gatgtttccc cggttgtcgg ggattggttt accgctgaac aggtgcaaca ttctttccag
     6301 atcggcgagt cggtcagcct gttgtccggt gataagatga aatccccacc ggtcccatct
     6361 caccagattg ttgacgataa ttttactgcc aaatttacag gggcaattgg ttttgtaatt
     6421 ccagttcagc cctctgaaga cgttgatcac ccctcgctca aacacctcat ccttattctg
     6481 gtgcagttgt tcaaatgtgc tcagaatgtt cgcttcgctg atttccggaa aattatcgta
     6541 ctccagtgac cggtaccatg tatcacgggc ctgcgtatcc attaatgaca gcatcccgga
     6601 acgttgcatc aggtcacgcc agatttcgcg atcaagattc cgggtaatgg ctttcattgc
     6661 tgtttcgggt ttctccatca gccagcagcc gcagcgaaaa tcctgtttca tggcccagtc
     6721 cggggcagtt ttacctccga tgctttccgt cagcaccgag atttcagcca gttgtcggat
     6781 aagtgtgtcg atttgcaaca gtgtactctt tcgtacagcc agaatgtggc gaatgctggt
     6841 tgagcagata acgtccgtat gcccgttgac cacttccgga cgggagattg tgatatctga
     6901 cataatcgat attccttttt gcagataaat gaaaacgcca gccctgtgca ggctggcgtc
     6961 agaggaatga agtctggtga gtgagcttca ccggttctgg tgcagaagct gcagatgacg
     7021 cagggtacgg acatcctccc gggcctgttc gatacagggc aacagtgctg ctgaatccgt
     7081 tttatcctca tcgttgtcaa agataattcc cgatccgcag tcgatattgt cctgcagcca
     7141 cgcaatcaga atatccagcg ctgtttccgt ggttaatgat ttcatgttgt tcctggttac
     7201 cagtcgaaag tgggtaaacc cggcaggcat tcagcgctgg catccagatg aatgagactg
     7261 acgccataac gccggatgag tgtgacgacc agacgacgga acgtttttga cagccccagg
     7321 cgtttaagcc gcagaaccgg gtatgaccac gcatcggtac gtaacagata tccgctacct
     7381 gtaaaatgaa tccattctga ttcaccgaag tcactggtct ggtgtgacag cgagtacaac
     7441 caggcgttgt ccgtttccgt gatatgtgcg gtactgcagc gtatgccggt aagggtagca
     7501 aatggtggcg tgcattctgc tgaggtctgg tcagattcat caacaacacg aagggagtag
     7561 ccgttatcgc agaccatgtt aatcagttca gcgagctcaa taccatcgac gtcaacgaca
     7621 atgcgcccca tgttcagtgc ctgtacgtta acgctgtcgg cttccagtgt cagggccagt
     7681 ttcatcattt cgcctccgga tacttaccca aggtaatgtt atttaccgtt ctgtaattgt
     7741 cgcgggtcat caggccggtc gcccggcgag cccggaggat atcaatactg tttattaact
     7801 gagagcgggg acaggtgctg aatcccggct ggtcggtacg caccagcgcg tatttttcca
     7861 cgagaaaatt aactgcatca cacagtgaaa tgcctgcctc aatatgctgc tcaatcacac
     7921 gttcatcggc aaacggtgtg tcattcagtg tgaggccata atgctggtcc agcagtcgga
     7981 tgagcagtgt ctgccagatg gtgacgggag acgggcagcg agatacctcc cgtacatgtg
     8041 tgtcggataa tgttttcatc ttaagttctc tgtaactggt agtgctaaag gtgagagctt
     8101 cggggttatg aggtggttgc gagtgctggt gtcggataaa cagccagata aacgtaacca
     8161 caggagccga gggtgtcggc ttcgcaggtc agcccttttg cattcagcgt gacggtatgc
     8221 tgatatcggg gattcagttc accgctggtg agcatgagtt ccagttgttt catcagcagc
     8281 ggaaaggcct ggtccagatg gtatgcatct gcgtcgctga agcggcctct gataccggcg
     8341 cggtcggcaa ggtaatgcag gcggttaccc tcttgcacca gacgagcgcc aaaacagggc
     8401 ctcactgtgc agggaagccc ccaccagggg cggtcgtgat tgtcatcggg atgagttatc
     8461 ccggagagtt tgtctgacac gataaaatcc tctctgaaaa gtggttaaga atggtttaac
     8521 gatgacaggt cagccggcgc gctgaccggt ttcggtgaag atgtatgcat tgatgagaat
     8581 ggcctcatcc acagcggcgt ctgaggtcat ctggtcgtac tcgtttcccg ggtgacggaa
     8641 catcagttca gggtgacact catcagccgg acatacgggc caaaactgtc cttacggcgt
     8701 tctgcaaaca cggcaagaac gccgggaata tcctgcactt cacgaccagt atacgcttca
     8761 gcactgccgt gccagcgata cttaccggta cagaacggaa aaagacggga tgtcggatgt
     8821 tgttggtgaa tacgcatggc ttcaccacgg gtgataattt tcataatggg atacctctga
     8881 agacagaaga taaaagtgaa aacaggtgtg atgtggttgt gaagggttaa agcagaccat
     8941 gttcggcaaa ggagaaaacc tggttgccac cgactatcag atggtccggc acccggatat
     9001 ccaccaggcc cagtgcctgt accagacgtt cggtgataag ccggtctgcc ttactgggtg
     9061 tgacttcacc ggacgggtga ttgtgcgcca gcaccacggc agcggcattg tggtacaggg
     9121 cgcgtttaat cacttcccgg ggatgaactt ccgtacggtt gatggtgccg gtgaagaggg
     9181 tttcaccggc aatcagctga ttctggttgt tcagatacag cacccggaac tcttcccgct
     9241 ccagtcccgc catgttcaga atcagccatt cccgtgccgc acgggtggag gtgaaggcca
     9301 cgccgggttc atgaagatgg cggtccaggg tttgcagggc ccgcagaatg agactgcgct
     9361 cgccgggcgt catctctccg ggcagaaagg aaagctgctg cattgtccct gcctccattc
     9421 agtcaatgat gcgcataatg gcgctgcatt ccggatgctg cagggcgtaa tcccgcagcc
     9481 ggtaatagtg gaccgtcatg gcataacact ccgtacgaca ggcatgatga ctgtacgtca
     9541 tcagacaggc agcaataccg gcggcttccg ggctcatttc agcgcggttg ccgttcatgg
     9601 cattgaacag tacccatgtt tc
//
LOCUS       DAZGLJ010000173         9533 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000173 DAZGLJ010000000
VERSION     DAZGLJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.173"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..104)
                     /locus_tag="ACHK49_003696"
     CDS             complement(<1..104)
                     /locus_tag="ACHK49_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264272.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKL"
     gene            182..1276
                     /gene="ddlA"
                     /locus_tag="ACHK49_003697"
     CDS             182..1276
                     /gene="ddlA"
                     /locus_tag="ACHK49_003697"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO9264273.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1300..1512)
                     /gene="yaiZ"
                     /locus_tag="ACHK49_003698"
     CDS             complement(1300..1512)
                     /gene="yaiZ"
                     /locus_tag="ACHK49_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO9264274.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1772..2080
                     /gene="yaiY"
                     /locus_tag="ACHK49_003699"
     CDS             1772..2080
                     /gene="yaiY"
                     /locus_tag="ACHK49_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO9264275.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2139..3233)
                     /gene="yaiW"
                     /locus_tag="ACHK49_003700"
     CDS             complement(2139..3233)
                     /gene="yaiW"
                     /locus_tag="ACHK49_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO9264276.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3246..4466)
                     /gene="sbmA"
                     /locus_tag="ACHK49_003701"
     CDS             complement(3246..4466)
                     /gene="sbmA"
                     /locus_tag="ACHK49_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO9264277.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4818..5975
                     /gene="ampH"
                     /locus_tag="ACHK49_003702"
     CDS             4818..5975
                     /gene="ampH"
                     /locus_tag="ACHK49_003702"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO9264278.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMVPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(5976..6599)
                     /gene="iprA"
                     /locus_tag="ACHK49_003703"
     CDS             complement(5976..6599)
                     /gene="iprA"
                     /locus_tag="ACHK49_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO9264279.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6687..9384)
                     /locus_tag="ACHK49_003704"
                     /pseudo
     CDS             complement(6687..9384)
                     /locus_tag="ACHK49_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556531.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(9414..>9533)
                     /locus_tag="ACHK49_003705"
     CDS             complement(9414..>9533)
                     /locus_tag="ACHK49_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO9264280.1"
                     /translation="REIMRATVFNYIECDYNRWRRHSLCGGLSPEQFENKNLA"
BASE COUNT         2477 a         2385 c         2241 g         2430 t
ORIGIN      
        1 attagcttcc cgttcatcat ttttgtgatc ttgcgcacat ctttgagata acgcactttc
       61 ttcaccagta cgttgcacgc tttgtcagca gaagattgca acaatctttc agaaggccgc
      121 gaatcccccg ttttgtcgtg gtaatgtatg cactttgtgt tagacgggat aggtttttaa
      181 gatggaaaaa ctgcgggtag gaatcgtttt tggtggtaaa tcagcggaac atgaagtgtc
      241 tctgcaatcg gcaaaaaaca ttgtcgatgc cattgataaa agtcgcttcg acgttgtgct
      301 gctgggcatt gataaacaag ggcaatggca cgtcagcgat gccagcaatt atctgctaaa
      361 tgcagacgat cctgcccata ttgcgttacg cccttcggcg accagccttg cgcaggtgcc
      421 aggtaaacat gagcatcagc ttatcgacgc gcaaaacggt cagccgttgc cgacggtgga
      481 tgtcattttc ccgattgtcc acggtacgct gggcgaagat ggttccttgc agggaatgct
      541 gcgggtcgcc aatttaccgt tcgtaggttc tgatgttctg gcttcagcag cctgtatgga
      601 taaagatgtt accaaacgtc tgctgcgcga tgccgggctg aacattgcgc catttattac
      661 cctgacgcgc gccaatcgtc acaacattag ttttgccgaa gtggagtcta aactggggtt
      721 accactgttt gtaaaaccgg ctaatcaggg ctcttctgtt ggtgtcagca aagtaaccag
      781 tgaagaacag tacgcaattg ccgtcgatct ggcgttcgaa ttcgatcata aagtgatcgt
      841 tgagcaaggg atcaaaggtc gtgagatcga atgcgcagtt ctgggcaacg acaacccgca
      901 agccagcacc tgtggcgaga tcgtactcac cagcgacttc tatgcctacg acaccaagta
      961 cattgacgaa gatggcgcga aagtggtagt tccggcagcc attgcgccag aaatcaacga
     1021 taagatccgg gcgattgccg ttcaggctta tcaaacgttg ggatgcgcag gcatggcgcg
     1081 tgtagacgtg tttttaaccc cagaaaacga agtggtgatc aacgagatca acacactgcc
     1141 tggcttcacc aacatcagta tgtatccgaa gctgtggcaa gccagcggtc tgggttacac
     1201 cgatctgatc acacgtttga ttgagctggc gctggagcgt cacgctgcgg ataacgcatt
     1261 gaaaaccaca atgtaatatt ttaacgcccg ataatattct tattgggcgt tttcagtttc
     1321 ttcatcgcga cgacgaataa tcagccccgc cagccagaaa ctaatcaccc acgtcaccag
     1381 accgacggca taggtctgcc aaccttttgc ttcaaatccc agtaacccca ccacgccatt
     1441 aagaatgaat ataaggccaa tggcgaacgc atagtagtgc cagtcacggc ggatttttac
     1501 aggcagattc atggcagctc cgttaacgaa aaacaacatc cttgcatatt ctgtaacaac
     1561 cgtctgtggt gtagacaaca ttttcgtaat tgttaataaa tttaacttaa ggttgccatt
     1621 ttgtgactca caacgaagat tcacgcttcg ggaatattcc caacatcaat ttaccatgat
     1681 tctgatattg acaatctaaa tgacctgtca gacttgccat aagccgctgg tatcacacca
     1741 gaagagaagc agatgacttc cggaggtttt tatggctgat ttcaccctgt caaaatcgct
     1801 gtttagcgga aaatatcgca atgcctcttc aacgcctggc aacattgcct atgcgttgtt
     1861 tgtgctgttt tgcttttggg ctggggcgca attgctgaac ctgttagtgc atgcgcccgg
     1921 cgtctatgag cgtttaatgc aggtccagga aacaggtcgc ccacgggtgg aaattggttt
     1981 aggtgtcggc accattttcg ggctgatccc gtttttagta ggctgcctca tttttgcagt
     2041 ggtggcgcta tggctgcact ggcgacatcg ccgccagtaa gatttacgtt gccagatacg
     2101 gcctaaaacg ccttatctgg catgacattc ctgtttgatc actgtttcat acaacgcgcc
     2161 cgccgttcgt ctacgcgctt cgcaaaccag gccgtagtca ggttgcgggt gattttcggg
     2221 ctttccagtt gaatgccagg caacatttcg cggggtagtg atttacccgt tttcgcttcg
     2281 gcaagttgat aaactttctt gtacagcgcc gtctcctcaa aagagaagct gtcacctttc
     2341 tctaactgac ggcgaatttc gctgtcgctc atccccagtt ttcccgccag tttgcgcgtt
     2401 gccagttcgg ttttcccggg ttctttactg tcgtaacgaa ttaaatcacc gtccagtgcc
     2461 agcttcacgc cgctggcctt actgactgcg ttctgaaacg cggcattacg gctggcgtac
     2521 cagccagcgt taaaatcagc aaagcggtat atcggtgcgc tatagctggc gggataattc
     2581 agtaaatggt aagtaccaaa ccacaacccg ccgcggcgac tgaagacttc ctgacgaact
     2641 gtaccgtcca ttttccacgg ataccctttg gtatgctgtt cggcaaaagc aatgctgacc
     2701 tgcatcggcc caccggtgcg cacaggatta agcgaaccaa acagcgtctg ccccattggt
     2761 accatgctga tcagatcgtc aaaaatcgcg ctcaattgct tttctgtacg caccgaatct
     2821 aaccgttcgc tataactttt gccgtttggc gatttgattt ttagcgcggt atgaaccaga
     2881 aaagcaggaa tgtgcatccg ttcagcacga cggtcaatct cttgccaggc gatcttgctt
     2941 aagcccggaa cggccggatc tgcctgataa tttgactcct gctgcgccac cgccagcacc
     3001 gagcagacgt tttccagcgt cggtgccagc ccctgacttt caaaggtggt ggcgagatct
     3061 ttcgcccagg catcgcggtc tttcacgctc gcaggcatct tctgccgcac gacgctcgct
     3121 acatctatcg ctttttcgcc ctttttcaac ggttgcggag cctgactact acaccccgct
     3181 aaaaccagaa ccgccaacaa tgacagcgaa gatagatgat tgacgcgcga cattacttct
     3241 cctttttagc tcaaggtatg ggttacttcc tgaattttgt caccatccag ctcatgttca
     3301 aagctgcgca gacgtttgta gatagacatc aactcaacca gtgtggtcca tgagttaatc
     3361 aggtactgga aagcaccgcg aacctgacca aaaacgttgg taatctgcgt catcaggccg
     3421 agcgtaatcg taccggcaac aattgacgga aacagcaaga acaaaccgaa aacgttatcg
     3481 acctgcaaat agagaatgcg ggcgatgttg aaatacatat agtgaaaata gaggcgaaaa
     3541 tagtttttcc gtacggcgct aaacagctcg cgtaccgtag gcggcgtcgc gcgcgtggca
     3601 tcgtcttcac cataaaccag ctctttacgg taggcggcct ctactcgctg gtttttaaac
     3661 tccagccccg gcagtttgat ccctaccact gccagcaatc cggtccccat cagcgaccag
     3721 acgatagcgg caatcaccag accatacgga atgtgcccga taatcggcag ctccggcaca
     3781 tgcgcggaga gcgttaccag caccggcaga aaggcgatca acgtcatgat ggcgttgata
     3841 aaactgacgc ccatattctc cagcgttgaa gcaaaacgca tggtgtcttc ctgcacacgc
     3901 tgtgcggcac cttcgatatg acgcagttgt tgccagttcg ccatgtaata ttcgttcatc
     3961 gccgtacgcc agcggaacac atagtgactg acaaagaagt tgttcagtac actgatcacc
     4021 accgcgatca gcgcaatccc cagaaagacg cccacttcgc ggtaaaattg ttcgatagtg
     4081 actttatgcg gcgaacttag cgcggtttga atcagatcat agaacggtgc ataccaggcg
     4141 ttgacggcga ccccgacttc caccaaaaac caggtgacga agatgatcag tgcagtaccg
     4201 agtattgacc agtattgcca acggtgcggg ctgtagataa accagaaaaa tgcaaaaagt
     4261 cctacgcaaa caatgtagta agcgtaaaaa atcaggaaat ccaacgacca gaaacgcgcg
     4321 gcgctaatcg ggatctgccc ggaagcgccg gtgatacgcg ccacccagtc acccccaccg
     4381 gcttgccaga agataacggc aatcaatgcc caaacaaagg ccgagagaaa aaacgttccc
     4441 ggctttggga aaaaagactt aaacatatgc actcctgcta acttcttatc gttttaacaa
     4501 ctgctgtgta ttaaccgcat gaccgctaca aagcgtcagc caaatgaaac cgcccattgt
     4561 gacccatagc aaggcgctta gttcgccagc ctgaccataa gaattgtttc gacggatttc
     4621 acaaaccaaa gattgtttac ataagcggat taacacgcgc aggatcacaa atgcaggcat
     4681 tggtgagcag gaaaaagatc gtgtttacgc gccgttacgt ttttaacact attattgcac
     4741 taattcgccc tttgcaatcc atcaatgagt agtataaata cgctcagtta ccttcattca
     4801 atctatggac accaccgttg aaacgtagtc tgcttttttc tgccgtgctg tgtgcggcgt
     4861 cattgacctc cgtccatgcg gcgcagccga tcactgaacc ggagtttgcc tctgatattg
     4921 tcgatcgtta tgccgatcat attttttacg gcagcggtgc cacggggatg gcgctggttg
     4981 ttatcgacgg taaccagcgc gtctttcgca gttatggcga aacgcgacct ggtaataacg
     5041 ttcgcccgca gctggattcg gtcgtgcgta ttgcttccct caccaagcta atgaccagtg
     5101 aaatgctggt gaaattgctc gaccagggga ccgtgaagtt aaacgatccg ctaagtaaat
     5161 atgctccgcc aggcgcacgc gtgccaacct acaatgggac gcccattacg ctggtcaatc
     5221 tggcaaccca taccagcgcg ctgccccgtg aacagcccgg tggcgcggca catcgtccgg
     5281 tgtttgtctg gccaacgcgc gagcaacgct ggaaatacct ttctacggcg aagctgaaag
     5341 ccgcgccagg cagtcaggcg gcgtactcta accttgcgtt cgacctgctg gctgatgcac
     5401 tggcgaatgc gtccggcaag ccttataccc agttgtttga agagcagatt acccgtccat
     5461 tggggatgaa agacaccacc tacaccccct caccggatca gtgccgccgt ttaatggttg
     5521 cagagcgcgg tgccagtcca tgcaataaca cgctggcggc aattggcagc ggcggcgtct
     5581 attccacgcc tggcgatatg atgcgctgga tgcagcagta tttgtcatct gatttttatc
     5641 agcgcagtaa ccaggcagat cgcatgcaga cgttgattta tcagcgcgcg cagtttacga
     5701 aagtgattgg catggatgtc cccggcaaag cggatgcgct tggtttaggc tgggtatata
     5761 tggtcccgaa agagggtcgt ccggggatta ttcagaagac aggaggtggt ggcggtttca
     5821 ttacctatat ggcgatgatc ccgcagaaga atattggcgc gtttgtggtt gtcactcgtt
     5881 cgccgttgac gcgctttaaa aatatgagcg atggcatcaa tgacctggtg accgagctaa
     5941 gcgggaataa accgctggtt atccccgcgt cctgattaat actctgaagg caaacggttg
     6001 atagcgacca gtttgccttt attcatttcg atatagccgc ctttacgcaa agcagcgaga
     6061 acttctgcga cgacagaacg cgatatgcgt gtacgttgat ggatataatt catcacacca
     6121 atacgtgatc gcaattcttc attccagtca atcattgata ataatgttgc gcggatttgt
     6181 tcgtaggaat tatgcccaat gagctgcacg tcgcgtaatt ccagaattct attttgccag
     6241 gctaaccagt aaaaagcgtc tcgccagagt tgattttgtt caataagcgt aatggtttgt
     6301 ttggctggta gatgatatcc cgtacaattt ccttctgata ttaatttgta tggtatatcg
     6361 tttttcatta aaccatcagc cagccccata atataaggag cctgggtaat accgataagt
     6421 acgttttctt ctctacgcag agagataact ccctctaaaa taacgaaagt atcttcgtta
     6481 ttgacatcac tggaaaatat aacttgcttt tcattattaa actcgaagcg cgtaccgtat
     6541 ctggacaaac atttatcgag cttaccaaat tcctgaagag gtttaactac agataacatt
     6601 tgcgcgtcct ttgcagtaat gcccgtcaaa tccttgacgg gcattattta gattaaatta
     6661 ccagaatttc ttcggagtga agaatattac caggtatatt taacacccac gttcgcggac
     6721 cagtcttgat ctacgtcacc accaccgagg tagttagcat cggtataggc gctgaagttc
     6781 ttggtgaagc tgaactgagt acccagacca acacgtaccg cagacccttc agtaccgtta
     6841 tcgatggaat caccgttcac atcgttatcg ttgttagagt cgtcgtagac gtaagccagt
     6901 ttgaagtacg gagtcagagc ctggtcttcg ctgtaggtga aggtataacc tgcatctaca
     6961 cccagttcat aacgcatgct gtcgtaagac tgaccgtcaa ctttcatgtc gttgctcagc
     7021 tggtagtcat caccagactg gaacagacca gaaacgctgc cgtaaggagt cacgtaacca
     7081 gcatcaccca gtttgaagtc gtaaccggct ttcaaaccga agccccaagc gtcggagttg
     7141 gtgctaccgt caacgtaagt accgttgctc atggttgcag acaggtcgtt gttgaagtga
     7201 gagtagctca agctaccatc aacaaagacg ttgttcgcga agtgagcaga agagtagatg
     7261 taggcagtct ggctgtcttg atccacctga ccagaacggt cattcatgtc acctttagcg
     7321 aagcctgcag ccgcaccgac gatccactta gcgttgttac cgtcaatttt ggtatcaaca
     7381 ccgaccatga tgccgttaac atcctgatca tagttgatgg tgccgttgtc gccgttgaag
     7441 ttaccaccga agtagcttac ccatgcgccg ccgttatcag ccaggccatg acgagagttg
     7501 gtcagacgag taccaacggt gtcttgttcc aggttccaga tattggtgtt cgcagacggg
     7561 atgctcagcg ccatgttagc gtagtcggtc agctccatct gttgcagaac aacggtgtta
     7621 ccgcgctgtt cagcctgata ggtgtatgca cccaggtcag ctttgttagc agcagagaag
     7681 gtcgcgttgg tgttgacgtc gtttacgtag ataatttctt tatctttgta atcagcgata
     7741 gcgccagcgc ctgttgcgtt gtcgatacga actttgtagt taccggttgc agcaacaacg
     7801 ttgtcaacag agctgttatc cagttcgtcg ccgttgttca tgtcaccgtt accgttgata
     7861 gtcagatgac catcagagtt catagcaaca ataccgtagc cgtagttaga cttggaagta
     7921 tcccaggtac ggtcgttcac caaatcagcg ttcagaacat aatcgctgct gtggatatcg
     7981 aatacaccag acactacgtt gccgttatgg tcagcgatgt tgccgctggt cagttccaaa
     8041 gtatcagtct ggaacggatt atcatcgaac tggtctacat taatatccag taaaccaccg
     8101 ttagtcacag ttattgtatt tgctaacagc gacggagtgt aataaccatt atagatactt
     8161 gaattgtcat cactagacaa cgctacagta ctgtagctat caactgtcag atgatcagtt
     8221 ccaacataac cttcatctcc aatgttcaaa gaagaaccat tggtcagagc gatgctatca
     8281 gaaatcaagc tagattcaga cacattaacc tgagagccat tattcacagc taaagtatca
     8341 atgtttgaag cgttaacggt attccattca gaaccattat tcagagtgac gttaaacaca
     8401 ccgctctgga atacttgatc atcaatcgta cgaccccaag catttgcttc tgttacaacg
     8461 cccggataca ggctattacc tgtaacatca taccagtcag tcgaagcagt agattcatca
     8521 tcaacatttg ctgatgtagt agctgagcct acccacttag aaccattatc cagagtgata
     8581 ttcagttcat caacattaag tgagtcatca gcccagccac cattggtgtc caagtacgcc
     8641 atcggcatta ctgtcatgac cttgaggata gaagtttgcg ttgaaagtac ttgcgaaaac
     8701 aacatcaccc agcagagtag aattgctgaa ataaacattg tttttcatgg agtaatttga
     8761 agccgcacta tcactgaaat acagagctac atcattagct tcgccgttac cagagtaatc
     8821 actaggttcc gcagagttac catagaaacc actgtcttcc agagtagtta cagaacctga
     8881 agtcactgtg gaatcggtaa ctgagatatt gttatcccaa gtatgagatt cagtagtgta
     8941 agtattgttt acggtattac cctgagtcag gctgatacca gcaacgcggg agttgttaga
     9001 aatattgatg ttactttcga catcaagatt cactgccagc ccaaggccaa caccaccgtt
     9061 tgcaaacaaa ccggcgtatt cttcaccttc accagctggg gtatggaagg tttcgttagt
     9121 atatttggta acgtcaccgt ccagatatga atcagtgaag tataaacctt cataatcgtc
     9181 atcaattgta gaattagcaa tatttaaagt gaatacatca ccatcatgcc aattatcatc
     9241 aattacacga tcagtaccgt attcagagaa ctgatagaca taatgaccat cactgctttc
     9301 acccataggc agcattgaag tgattgaacc atgaatcagg gagttgctga tgttcaactg
     9361 gtttgttgtt ccatcagtaa agttgatcct acccacgtaa tatggacaca ggcctaagcg
     9421 aggttcttgt tttcaaattg ttccggactg aggccgccac acaaactgtg ccgccgccac
     9481 cgattgtaat cacattcgat ataattaaat accgttgccc gcattatttc ccg
//
LOCUS       DAZGLJ010000174         9439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000174 DAZGLJ010000000
VERSION     DAZGLJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.174"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(111..1598)
                     /gene="yeeO"
                     /locus_tag="ACHK49_003706"
     CDS             complement(111..1598)
                     /gene="yeeO"
                     /locus_tag="ACHK49_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO9264281.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAVAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
     gene            1759..1834
                     /locus_tag="ACHK49_003707"
     tRNA            1759..1834
                     /locus_tag="ACHK49_003707"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1792..1794,aa:Asn,seq:gtt)
     gene            complement(1872..2822)
                     /gene="cbl"
                     /locus_tag="ACHK49_003708"
                     /pseudo
     CDS             complement(1872..2822)
                     /gene="cbl"
                     /locus_tag="ACHK49_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
     gene            complement(2924..3841)
                     /gene="nac"
                     /locus_tag="ACHK49_003709"
     CDS             complement(2924..3841)
                     /gene="nac"
                     /locus_tag="ACHK49_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO9264282.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4167..4242
                     /locus_tag="ACHK49_003710"
     tRNA            4167..4242
                     /locus_tag="ACHK49_003710"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4200..4202,aa:Asn,seq:gtt)
     gene            complement(4286..5233)
                     /gene="ldtA"
                     /locus_tag="ACHK49_003711"
     CDS             complement(4286..5233)
                     /gene="ldtA"
                     /locus_tag="ACHK49_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO9264283.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMFYERW"
     gene            complement(5295..6374)
                     /gene="cobT"
                     /locus_tag="ACHK49_003712"
     CDS             complement(5295..6374)
                     /gene="cobT"
                     /locus_tag="ACHK49_003712"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO9264284.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCAAHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6386..7129)
                     /gene="cobS"
                     /locus_tag="ACHK49_003713"
     CDS             complement(6386..7129)
                     /gene="cobS"
                     /locus_tag="ACHK49_003713"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO9264285.1"
                     /translation="MSKLFWAMLSFITRLPVPRRRSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7126..7671)
                     /gene="cobU"
                     /locus_tag="ACHK49_003714"
     CDS             complement(7126..7671)
                     /gene="cobU"
                     /locus_tag="ACHK49_003714"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO9264286.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(7823..7996)
                     /locus_tag="ACHK49_003715"
                     /pseudo
     CDS             complement(7823..7996)
                     /locus_tag="ACHK49_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            complement(7975..8163)
                     /locus_tag="ACHK49_003716"
                     /pseudo
     CDS             complement(7975..8163)
                     /locus_tag="ACHK49_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(9160..9439)
                     /locus_tag="ACHK49_003717"
                     /pseudo
     CDS             complement(9160..9439)
                     /locus_tag="ACHK49_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2594 a         2529 c         2014 g         2302 t
ORIGIN      
        1 ctacagagga atcgtgagaa cgaggcgaat attagcgatg cccacccaca atgtcaaagc
       61 ctgtttttta aatttgaaat cgtttgctga aataatctgc attttgttgt ttattccgac
      121 acaactggct ttttttcaca cttttgcggt tcgggtcgag ggtatttcca tagccaacgt
      181 ccagtaacca ttcgccagta aaacagcacg gcccgcacag cccagtcggc aaacattccc
      241 atccagacac caaccacacc ccagccaagc atgatgccca gcacataacc gacgacaacc
      301 cggcaacccc acatgctcaa catagaaacc cacatagcgt aacgggcgtc acgagccccc
      361 ttaaatccag cgggtagcac ccatgaggcg gaccaaatag gcataaataa agcatttagc
      421 caaatcagaa tcacaacgac atgtttaacc tgtggatcct gggtgtaaaa cgatgccata
      481 accccggcaa agggagccgt tagccaggcg atggccgtta atccaagagt ggacagccag
      541 aagacatgcc gcaactgaat ctctgcttgc gctatctgcc ctacccctaa ccttcggcct
      601 gtaatgatcg tagaagcaga gccgagcgca cttccgggta agttgataag agccgcaatt
      661 gaaaacgcga taaaatttcc ggcaataaca ctggtcccca tcccggcaac gaacatttgg
      721 gttaataacc gaccactggt aaataacact gattcgacac tcgctggaat accaatcccc
      781 atgacttccc agataatgct aaaattcagc ggtttaaaat agctctttaa cgaaatcctt
      841 agcgcaggat taaaaccaat cgccagcacc cacaaaattg caactgcgcc aatataacga
      901 gaaatggtta aacccaaccc agccccgaca aatcccagtc ccggccagga gaaaaggccg
      961 taaatcaata tgccgctaat aataatatta agaatattca ggctaccgtt aatcagtagc
     1021 ggtattttcg tattccctgc accacgaagt gccccgctac caatcaaagt gatggcaact
     1081 gctggataac tgagtaccgt cagctccaga taagtcaacg ccagtgcttt aacttctgtc
     1141 gtggcatcac ccgcgacgaa atcaataatt tgttcgccaa aatgatgaat aagcgttgcc
     1201 aacagtacgg caaacaacgt catgatcacc aatgactgcc gcgtcgccac cctcgctcgt
     1261 cgtcgatccc gcttaccgag actaaatgcc acaacgacag tagtaccaag atcgatagca
     1321 gcaaaaaaag ccataatgac catattgaag ctgtccgcca atcccacgcc ggccatcgca
     1381 tcttttccca gccagctgac cagaaaagtg ctcaggacac ccatcaacag gacacaggca
     1441 ttctccatga agataggaac agcaagcggg gttatctcgc gccagaacaa cactttgtag
     1501 ctcttgcgtt tagcgtgcca gcgagtgccg tgaacaacct ggcgtaaagc agaggagata
     1561 ttcaaagccg accttaattg cagaaagtga aaccacattt caaataatga gggagaatca
     1621 gcaaagctgc aaagattttc gccaacaaat tgtctgcaaa tgcaacaaac tgttgataga
     1681 aacggcaaac agttgggaaa tttaaaaatc gggtttgaca aaagattttt cgccgttaag
     1741 atgtgcctca acagcgattc ctctgtagtt cagtcggtag aacggcggac tgttaatccg
     1801 tatgtcactg gttcgagtcc agtcagagga gccaaatttc tgaaaagccc gcttttatag
     1861 cgggcttttt gctatatctg ataatcaatt tcctcttcac tgttctccat cacctggcgc
     1921 ttgatatcct caactgacag tcctgcatta caaagttcca gaaagcgcca gacatagtta
     1981 cgttgaagtt gccctcgctt cagtcccaac catacagtat tggcatcgaa aagatgccgc
     2041 gtatccagac ggattaaatt ctcttcctct tgttcgccgc tggattgttc ggcaactaat
     2101 ccaatcccca gcccaagagc aacataggtt ttaatgacat cagaatcctg cgcacttaat
     2161 acaatatctg ccagcaaacc tttgcgggca aatgcgtcat caatacgtga gcaccccgta
     2221 atcccctatc ggtaagtgat taacggccac ttcgctattg attccagcgt caacggtgta
     2281 atttgcgtca agggatgatc gagtggaaca agcaaactat ggtgccaacg aaaccacggg
     2341 aaggcgacga gatgcgggtc attactcaaa cgctcgctgg cgataccaat atcagcttcg
     2401 ccattttgca acaacgtcgc aatttcctgt ggcgtaccct ggattagctc cagccgaacc
     2461 tccgggaaaa gttcgcgaaa agctttaatg acctcaggca agctataacg tgcctgagta
     2521 tgcgtcgttg caatagtgag aacgccagac gtatcgttgg taaacaggtc tgcaagccga
     2581 cgaacattac tggcttcatt cagaatacgt tctgcaatga ccagtaatgc tttgcccggt
     2641 tcagtcatac ccagcagtcg cttacctcgt cgaacaaata tttcgatgcc aagttcatcc
     2701 cccagttccc gaatatgacg gctgacgccg gactgtgagg taaaaagcat attcgcaacc
     2761 tctgtcaggt tgtaatcctg acgtgcagcc tcgcggatta tctttagttg ttggaaattc
     2821 acggtaaact ccgggcagct cagatttccc gttattgtta aagtctaatg cccggcataa
     2881 caaataataa aaacccgcat cttattccat cccgatataa cgcttagctc accaattgcc
     2941 actgcctttt ttccatcact ggagaactaa tcactgacat taacaactct ttcactgcct
     3001 gtgcctgtgg cgataagttc gctctggcgg gtaaatttaa tgacaaagag agactcatgg
     3061 aaggcgtggt aatgcgtgac atccacccat ttactgcgcc acataacgaa cgcgcggccg
     3121 attcgggtaa tactgcaacg cccatgccgc tggcaatcgc tgcggtaagc gtggcaatag
     3181 actcaatttc accaatgact tttgccgtga gccgccgtag ggaaaaagcc tcatcaacac
     3241 gaagtctaac agcactgtaa tcactgggga gaaagaggtt catttgcgca atagcattaa
     3301 catcaacgct ttgccccggg caatcttgag ttcctaccag gaaaagatct tctttcagca
     3361 aagcctgact ggatacccca gccacagggg aatgctcata aatcaccgcc atatcgagtt
     3421 ggtgatttat cagtttttcg ttaagcactg caccgctatt ttcatgaaga tagataacga
     3481 tctccgggaa ttcagcgcga accgcttgta ataagggcat ggtgatagat gacgcagcgg
     3541 ttcctggcgc aaagccaatc gagacttgcc ccgataatgc ctgaccaacg ttatgcaccg
     3601 ccagttgggc ctgctcacac tgacgtaaaa tggcccgcgc atgggtatag agaatttttc
     3661 cggcgtctgt tggcgtaacg ccccgttttg tgcgaatcaa aagttgttga tttaactcac
     3721 cttccagtgt ggcaacctgc tggctgagcg ctggttgtgc gatatgcaat acttcagcag
     3781 cctgagtcag gctaccaata tctacaattt ttacgaagta tttcaggcgt ctgaagttca
     3841 tgttgcctcc ggtttttgag aatcggccca agtgccgcca ttacttacaa ccagactgca
     3901 agatgcttgc cagttttatt ttggcgttga tgtacaagct aaccaactgt caaataagag
     3961 attatgatag attcgtcatt ggctcttttc accagcagtc gcgttcccct gccctataaa
     4021 aggagggtat gcaccacgat ggttcattac caataagatt gaaatctcat cactttgttg
     4081 aaattggcag caaacaaaca aaaaaatgca tttcaccctt tgacatcacc atgcactgcc
     4141 attaatatgc gccccgttca cacgattcct ctgtagttca gtcggtagaa cggcggactg
     4201 ttaatccgta tgtcactggt tcgagtccag tcagaggagc caaattcaaa aaagcctgct
     4261 ttctagcagg atttttgctt tctaattacc aacgctcata aaacatctgt cttgaaccag
     4321 aactaatttg cacaggcatt cccgatcgac gttgcaacgc agcatttgcg cgattcacat
     4381 caacttcttg cccgttgata aacgcccgca aagatggggt taccggcaat ggcacttttc
     4441 ggtcagactc atattctgca cgattgcgcg acagcggctc atgaacttcc agccagttcg
     4501 agccatctgg ttcagtggtg tattttactg gctggtcgat aatttgcaca cgcgttccaa
     4561 caggaacatt atcaaacaga tatttgatat cgtcattgcg cagacgaata cagccctgac
     4621 ttacccgaag tccaatacca aaattggcat tggtaccgtg gatggcatac aacctgccaa
     4681 tataaatcgc gtacagcccc atgggattat cgggccccgc aggaacaaat gcgggcaaac
     4741 tctcccctcg tttcgcatat tcgcgccgag tgttcggcgt tggcgtccag gttggagctt
     4801 cttgtttacg ttcaacggta gtcacccagt tacgcggggt ttctcgccca gcctggccga
     4861 taccaatagg aaagacttcc acagtattac tgtctggcgg gtagtaataa agacgcatct
     4921 cagcgacgtt aaccacaatc cctttacgaa cagtggcggg caaaatcagt tgctgcggaa
     4981 tggtgagttg cgagccagac ttcggcaaga aaacatcagc gcccggattc gcttccagca
     5041 tgttacttaa cccttgcccg tattgtgcgg caaaagtctc cagcggctgg gtattgtgat
     5101 caggaacagt tacagtaagc gactgcccca ccaaacggct accctctgga ggtaatggat
     5161 aagttaccgc caggctagta tggctggcaa aaagcagagc aaatgagcaa agaatattta
     5221 cacgacgcat catgtccctt tcctatgtcg cgaaagctat ccgttaagta tagcttttat
     5281 cagacttttc gtttttaact gttcaaatca gaagtcgtat tccccggtag aacaatatta
     5341 ctggcagcaa gttcgcccat gttgttatat atcgcacagg cagcttcgat gatgggcatc
     5401 gccagagctg cgccactccc ctcacctaaa cgcatctcca tattgagata aggctccagc
     5461 cccaaatgcg acagcgctat acgcgcccct ttttctgccg acaggtgaga aggaatgaga
     5521 tacggtttga ttgcaggaga catctggcag gctgcgagcg cagcagcata agaaagaaat
     5581 ccatccagca acacgggtaa accgcaggaa gcagcgccta acatcacgcc agccattccg
     5641 accaaatcaa atccccccac ttttgccaga acatcaacgc catcctgcgg atttggttga
     5701 ttcaacgtaa tcgcccggcg cacaacatca attttattag ccagtttatc tgtcggcagg
     5761 tttgcgccaa tcccaaccac ttcttcagga tcccggccag tgattgtgct gactattgcc
     5821 gctgccggtg tcgtgtttgc catccccagt tcacctacac caaacagcgt gacaccgttt
     5881 tttgccagct cccgcgtata acatatgacg tccaaaagca acttttcagc ctgacggcga
     5941 ctcattgccg gagctgaagc aatattgccg ctacctcgtg cgacacgcat gttgataagc
     6001 ccggggatag gctcagcact atcaatacca acatcaacta cgtggacgtt agcgcccgct
     6061 tgtgctgcca gcacacacac gccggttgtt ccgcgggtca tattttcagc ctgaatggct
     6121 gtcacttctt ttggggaaat agcgacccct tcctcccaga cgccgtgagc ggcacacata
     6181 accagtaccg cttttttgcc cacatgcggt atgccattca accccggcat tcccgccagt
     6241 tgtatggcaa gcgcctccag ccttcccagg ctaccaacag gtttgagtaa cccgtcaata
     6301 tgccgttgtg cacgcgacat agcggcagga tcgatagcag ggatcgtatt cagtaaatcg
     6361 gcaagtgttt gcatgtcacg tctctttata gcagtgccag caggaagacc agttcaccaa
     6421 gctctatagc agctcccagc gtatcgccgg tttgcccgcc cagcgtacgt ttgagaagct
     6481 gaccgaggat gaaaatcgcc accatcgtca ccaccatagc agccacacca tgcatacccg
     6541 gcaataatac ggcagcaaaa atagcggcta agccgagtgt gacgcaggtt tgtcgcccat
     6601 caattttgcc aatgaacaca ttgccaagcc cttcttcccg ggcataacga tgacgataca
     6661 tcaataatgc ggcagtcccg cggctgaccg cacatgctgc cgccagcgat gcaaggatcg
     6721 gttcgccacg cagagccaac tcgcttaata ccagaatctt tgccagtaca acaaaaatca
     6781 atgccagacc gccatgggtg cctaaacggc tatcacgcat gatttccaac atgcgatcgc
     6841 ggctacgtgc agaaaatacg ccatcacagg tatcggcgag gccgtctagg tggaatcccc
     6901 cggtcatcag cgccagcaca agtacgctaa acagtgccgc cagtggtacg ccacaccatg
     6961 cctgcagcac catgaagacc agcccgctaa tcgcgccaag caataatcca attaaaggaa
     7021 aagtaataat accgcgagaa taatgctcga aatccagtcc ctgggacctg cgacgcggta
     7081 caggcagacg cgtaataaat gagagcatcg cccaaaataa tttactcatt tgatttttac
     7141 tccaataccc gaaaccacca gccatacttc atttgccgca gcggccaact gctgatttac
     7201 ccgcccggca atatcacgaa aatgtcgtgc cagacgactc tccggcacaa tccccattcc
     7261 cacttcgtta gtcactaata caacctttgc ggggcaacgt tggcaggcag caatcaacga
     7321 ctgaatctca gcattaatcg cctgttccat cgcctgataa tcccattcat cagggtcttt
     7381 atcgccgcca taatcaaaca acagattagt caccattgtg gtaacgcatt caagcaacac
     7441 aacctcattc gggttaatgt ctgcatgaat taattcatca agatgttgcc agcgctccac
     7501 tgtgcgccag tgctccgggc ggccttgccg atgatgttct atccgtgcag ccatctcatc
     7561 atcaaggatt tgcgaggtag cgatatacag aacctgtgaa gagtccccaa taagcgcctc
     7621 tgcgtggcga ctcttcccgc tccgtgcgcc ccccgtcacc aaaatcatca taccgactcc
     7681 tgattcaaat gtatgatggt attcattgaa cagaacaatg gatcccccac cgttgctgac
     7741 cgcaaaacat ttacgcgatt aatactgcgc gtaatataat taaaaatcca gcattctcaa
     7801 tccattacga aataagatgg cattacggaa tagccagact ttctcgtgtt aacgtccgac
     7861 aaaatgcgtc attgacagcg gtttctgtgg attgacacat ttcattcaga taatgaatta
     7921 atgctactgc cggaacaacc agcaaacggg cattgtgttc tgaaatccag gctattgatt
     7981 caacgtcagc gacagatctg cgctgtaaaa gaacttgtaa aacaacgtaa tagaattatc
     8041 cggaatggtg gcgactatgc actagaacac cacaatttcg ctctctcggt aaggcactgt
     8101 cacagcattc aacagaatgt gacttgccag atttattagc gccaccagat gtttaaccgg
     8161 gtaaccacga cccagtcggg gatatattcc atcttctcgc tgacatcatc aagaataaaa
     8221 aggtttatca cactcagagc aacgttcttc agcaggcggg ataattcttc aacatttaga
     8281 agatgtgtta ttaaaggcat acaaactgga cgtattgtat tttcttttgg tggttgacct
     8341 aaggtagcag tttatcctga tgcgctgaga tttctgtaat atctacgtca acattctcct
     8401 cgaatagaaa atgcagcccc ggcaagtgat tcacattttt taacaaaacg ttgttagcgt
     8461 gccattttca gaagctctcc aagaaagcga attcttttct caaattctgc attctcagac
     8521 acagctttct actccatggt aatggcccaa atacgaagtt gctcaggatc attagtggtg
     8581 ctgagaagtt aaataaccat gcggagtacc actcgtcatc acgtatttat atatcatgaa
     8641 gctattgttg tgttttgtac atcagtagat atattctgat atactccttt tgctagacat
     8701 aacctttcac ctgcttgcaa agcttctgtg ttctgacatt gccaaattgt tgcaattctg
     8761 tatccagcct tctttcagtc atagcttcgg gccgcgataa gactcactga tctgaccctg
     8821 attcctcttg cagactttat agaccaatta aaatgcagtt tctgcaggtc aacgtctgac
     8881 catcattgtc atcactctgg ccattagagt aaccttctgc attcatcctt ttgtaaaaag
     8941 tttatgtcag tatcagcaat taaccggacc tgatactgat atgagtctta ccgcatatac
     9001 ggtcaatttc agcaattaat tacattatcc acgccaaagt gtttgtcatc gcaatgatgg
     9061 taccttcttt cagacaccat tttttcaact ccgttttcca cggaccgcac tcttatgtca
     9121 agagtgcggt gatcctaccc acgtaatatg gacacaggcc taagcgaggt tctggttttc
     9181 aaattgttcc ggactgaggc cgccacacca actgtgccgc cgccaccgat tgtaatcaca
     9241 ttcgatataa ttaaacaccg ttgcccgcat tatttcccgg ctgataaagt gttctccatg
     9301 gatacattcc actttcagcg aatgaaagaa gctttccacg cagattatgc cgcttcagtt
     9361 gcgcctgata atctgctgaa cagtactggc ctccacggtc cgtgtgaacg ataacgttcc
     9421 ggggcctctt acgccgcca
//
LOCUS       DAZGLJ010000175         9373 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000175 DAZGLJ010000000
VERSION     DAZGLJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.175"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(277..1224)
                     /locus_tag="ACHK49_003718"
     CDS             complement(277..1224)
                     /locus_tag="ACHK49_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFO9264287.1"
                     /translation="MPELTSLFPDVANALDIESVAIVEKDYFVVNLLKLLKEIKPETH
                     TLVFAGGTALSKAGISLNRMSEDIDIKLVPTENFMQNGRNRKRKIRKEIVQIITDVLH
                     NSDIFSLDNENARITRDEYRYNEIPVRYPQTFAQAPCLRPFIKLELMETTLLEHPESR
                     DIYSLVTELTGNGTPVTAFPCATILSTQAEKLISMMRRTAILLRNPEQQDDESLVRHI
                     YDNYCIVREKGVNVPVLKNFVQICIQQDIERYGNQHPQFRNSPVDELKKGLDALRDDP
                     VHKLRFQRFVKPMVFGNTQIDWEEAYSCFRQTALSVLTN"
     gene            complement(1217..1612)
                     /locus_tag="ACHK49_003719"
     CDS             complement(1217..1612)
                     /locus_tag="ACHK49_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6088 family protein"
                     /protein_id="HFO9264288.1"
                     /translation="MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEG
                     KLMKVGYGVYTKARRNVITGKIMPAAPGGSAAVITEALDRLNIRYSLIGATECYNKGQ
                     STQIPVSPEFKTPPRFKRVLVVGKSKINA"
     gene            complement(1681..2526)
                     /locus_tag="ACHK49_003720"
     CDS             complement(1681..2526)
                     /locus_tag="ACHK49_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001440175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO9264289.1"
                     /translation="MSDITASRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTARDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVLNVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIRLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKYYPN
                     VLTER"
     gene            complement(2611..2808)
                     /locus_tag="ACHK49_003721"
     CDS             complement(2611..2808)
                     /locus_tag="ACHK49_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO9264290.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDRTDSAALLPC
                     IEQAREDIRTLRQLQLLQQNR"
     gene            complement(2826..3314)
                     /locus_tag="ACHK49_003722"
     CDS             complement(2826..3314)
                     /locus_tag="ACHK49_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO9264291.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGVTLAELINVVCDNGYS
                     LRVVDESDRTSAERTPPSAALTGIRCSTAHITAKDNAWLYSLSHQTNDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLIVTLIRHYGVSLIHLDACAECLPGLPTF
                     DW"
     gene            complement(3311..3688)
                     /locus_tag="ACHK49_003723"
     CDS             complement(3311..3688)
                     /locus_tag="ACHK49_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO9264292.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMARDNYRTVNNITLGKHPGAKQ"
     gene            complement(3778..4146)
                     /locus_tag="ACHK49_003724"
     CDS             complement(3778..4146)
                     /locus_tag="ACHK49_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO9264293.1"
                     /translation="MSDSLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELSPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYLAVYPTSETKT"
     gene            complement(4196..4840)
                     /locus_tag="ACHK49_003725"
     CDS             complement(4196..4840)
                     /locus_tag="ACHK49_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HFO9264294.1"
                     /translation="MSRIITTTVYTLNELSSTAREKARDEYRQHHADSNWYENVYEDF
                     REVCDIFGIDLRQRVIRLSNGRFMEEPCIWFSGFCSQGDGACFEGLWHWQPAAPRKIR
                     EYAPQDRELHRIADALQAVQKRNFWQLQAEISHRGRYCHPYSMDITVTRNSPTGQVMT
                     TDAEAAVSEVLRDLAFWLYRQLENEYDWLTSDAAVDEALLINEYTFTEAGLRAG"
     gene            complement(4859..5080)
                     /locus_tag="ACHK49_003726"
     CDS             complement(4859..5080)
                     /locus_tag="ACHK49_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO9264295.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(5149..5625)
                     /gene="radC"
                     /locus_tag="ACHK49_003727"
     CDS             complement(5149..5625)
                     /gene="radC"
                     /locus_tag="ACHK49_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO9264296.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VILAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(5641..6126)
                     /locus_tag="ACHK49_003728"
     CDS             complement(5641..6126)
                     /locus_tag="ACHK49_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9264297.1"
                     /translation="MTTSSHNSTTPSVSVTAASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNV
                     MNGNRAEMSPEAAGIAACLMAYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(6203..7021)
                     /locus_tag="ACHK49_003729"
     CDS             complement(6203..7021)
                     /locus_tag="ACHK49_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO9264298.1"
                     /translation="MQLASRFGHVNQIRRERPLTREELMYHVPSIFGEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDKHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLENLR"
     gene            complement(7111..7344)
                     /locus_tag="ACHK49_003730"
     CDS             complement(7111..7344)
                     /locus_tag="ACHK49_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO9264299.1"
                     /translation="MSDCHPVLLPEGPFSREQAMAVTTAYRNVLIEDDQGTHFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNSYLASEGILRQ"
     gene            complement(7350..8027)
                     /locus_tag="ACHK49_003731"
     CDS             complement(7350..8027)
                     /locus_tag="ACHK49_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264300.1"
                     /translation="MNTRILFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEIGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARQAYARKYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVIVTVGADGNATGA
                     SVEFIGK"
     gene            complement(8146..9030)
                     /locus_tag="ACHK49_003732"
     CDS             complement(8146..9030)
                     /locus_tag="ACHK49_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO9264301.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQLGERRMTLVDLPGIGETPQH
                     DQEYRELYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNDTEKCPSRQQELSLATVTARVATLFPSSFPVLPVAAPVGWNLPAFVSLMI
                     HALPPQATSAVYSHIRGENRSEQARKHAQQTFGDAIGKSFDAAITRFSFPAWILQLLR
                     KTRDRIIHLLVTLWDHLF"
BASE COUNT         2211 a         2280 c         2492 g         2390 t
ORIGIN      
        1 ggcaggacgc ttaaatcgtg gcgtcctgaa acgaaaaacg gacctccgtg gaggtccgtt
       61 tatatgaatt tggtgcccgg actcggaatg aaactggttg tataacaagt tgttttatat
      121 tgattgtttt gtgtttgtta tttttgaggc caccaatgag gcccccatgt gcaattcgaa
      181 ttcttttgac gcgataatcc ctcttttttg tgcttggctg taattatttt gtctggttcg
      241 atgtgctgat gcgcggaatc gtacttaacg cgtttgttag ttggtcagaa cactcaacgc
      301 tgtttgtctg aaacaggagt aggcttcttc ccagtcaatc tgtgtattac caaataccat
      361 cggtttaaca aatcgttgaa aacgcaattt gtgtacagga tcatcccgga gtgcatccag
      421 ccctttctta agttcatcaa ctggtgagtt acgaaattgt gggtgctggt taccatagcg
      481 ctcaatgtcc tgttgaatac atatctgaac aaaatttttc agtactggaa cattcacacc
      541 tttttccctg acaatgcaat aattgtcgta aatgtggcgt accagggact catcatcctg
      601 ttgttcggga ttgcgaagaa gtatggctgt tcttcgcatc atggatatga gcttttcagc
      661 ctgagtggat aaaatggtgg cacaggggaa tgctgttaca ggtgtgccat tacccgttaa
      721 ctccgtgacg agtgagtaaa tatctctgct ttccgggtgt tccagcagag tggtttccat
      781 caactccagc ttaataaatg gacgaagaca gggggcctgg gcaaatgtct ggggataacg
      841 tacggggatc tcgttataac gatactcatc acgtgttata cgggcgtttt cattatcaag
      901 gctgaaaatg tcagaattat gaagaacatc cgtgattatt tgcacgattt ccttgcgaat
      961 ttttcttttc ctgtttctgc cattttgcat aaagttctct gttggcacga gctttatatc
     1021 aatatcttct gacattctgt tcaatgagat accagctttt gacaatgcgg tacctcctgc
     1081 aaacacgaga gtatgtgttt ctggttttat ttcttttagt aactttaaaa gattaaccac
     1141 aaaataatct ttttcgacga tggcgacaga ttcaatatcc agtgcgttgg caacgtcagg
     1201 aaataatgat gttaattcag gcattgattt tacttttccc tacaactaac actcttttaa
     1261 agcgaggagg tgttttgaac tccggagata ccggaatctg tgtggactga cctttattgt
     1321 agcattctgt tgcgccgatc aagctgtatc tgatattcaa acgatccagg gcttcggtga
     1381 ttacagcggc agagccacca ggtgctgcag gcatgatttt tcctgtaata acgttgcgac
     1441 gggctttggt gtaaacacca tagcccactt tcatcaactt accttcgttt accagcgtac
     1501 gaagagctct gccaacctga tcatagccag cgatatccct gaagtcatcc cgggtgaaca
     1561 cataacgctt tgaacggttc agtcgggact gaatacggtc ttttgtagtc atgcttcctc
     1621 cgaaaaatat taagaatgat agcagtaata cggcatgttt aatagcgggg cagggtcaca
     1681 ttacctttcg gtcaatacat ttggatagta tttcgcaata atgtcattca gtctgtcaac
     1741 cagctctggt ttcctgaacg tgatgtgcgc ggagcccttc ttaaagtatc tgatgctgaa
     1801 catctcatct tcatagtcct ctttaccctg aacagactgg atgtgatcat ccagacgaat
     1861 agtgatgttt tcccggttgt cggggatcgg tttgccgctg aacaggtgta gcattctttc
     1921 cagatcggca agtcgatcag cctgttgccc ggtgataaga tgaaaccccc accggtccca
     1981 cctcaccaga ttgttgacga taattttact gccaaatttg cagggtgaat tggttttgta
     2041 attccagctc agtcctctga aaacgttgag cacccctcgc tcaaacactt catctttgtt
     2101 ctggtgcagt tgttcaaacg tgctcaggat atttgcttcg ctgatttccg gaaaattatc
     2161 gtactccagt gaccggtacc atgtatcacg ggcttgtgca tccattaatg acagcatccc
     2221 tgaacgttgc atcaggtcac gccagagttc gcgatcgaga ttccgggtga tggctttcat
     2281 tgcggtttcc ggtttctcca tcagccagca accgcagcga aaatcctgtt tcatggccca
     2341 gtcccgggcg gttttaccgc caatactttc tgtcattgct gatatttcag ccagttgccg
     2401 gataagtgtg tcgatttgca gcagtgtact ctttcgtaca gccagaatgt ggcggatact
     2461 ggttgagcaa ataacgtccg tatgcccggt gaccacttcc ggacgggagg ctgtgatatc
     2521 tgacatcatc gtttttcctt ttggtttata aacgaaaacg ccagctgtat tcaggctggc
     2581 gtcaggggaa tgaagtatgg tgagtaagac tcaccggttc tgttgcagaa gctgtagttg
     2641 gcgcagggta cggatatctt ccctggcctg ttcaatgcag ggcaacagtg ctgctgaatc
     2701 cgttctatcc tcatcgttgt cgaagataat cccggattca cagtcaatat tgtcctgcag
     2761 ccacgcaatc agaatatcca gcgccgtttc tgtcgttaat gatttcatgt tgtgaatttc
     2821 cctgattacc agtcgaaagt gggcagaccc ggcagacatt cagcgcaggc atccagatga
     2881 atgagactga cgccataatg gcgaatgagc gtgacgatca gacgacgaaa cgtttttgac
     2941 aggcccaggc gtttaagtcg cagaaccggg tacgaccacg catcggtacg taacagatat
     3001 ccgctaccgg taaaatgaat ccattctgat tcgccgaagt cattcgtctg atgtgacagc
     3061 gagtacagcc aggcgttgtc ctttgccgtg atatgcgcgg tactgcagcg tatgccggta
     3121 agggccgcag atggtggcgt gcgctctgct gatgtccggt cagattcatc aacaacacga
     3181 agggagtaac cgttatcgca gaccacgtta atcagttcag cgagagtaac accatcgacg
     3241 tcaacgacaa tgcgccccat gttcagtgcc tgcacgttaa tgctgtcggc ttccagcgtc
     3301 agggccagtt tcattgtttc gcccccggat gtttacccag ggtaatgtta tttaccgttc
     3361 tgtaattgtc gcgggccatc aggccggttg cccggcgggc ccggaggata tcaatactgt
     3421 ttattaactg agaacgggta caggcgctga atcccggctg gtcggtacgt accagtgcgt
     3481 atttttcaac gagaaagttc accgcatcgc acagtgaaat gcctgcctca atatgctgct
     3541 caatcacacg ttcatcggca aacggtgtgt cattcagtgt gaggccgtaa tgctggtcca
     3601 gcagtcggga cagcagtatc tgccagatgg tgacgggaga cgggcagcgc gatgcctccc
     3661 gtacatgcgt gtcgggtaat gttttcatac tgaagatttt cctgatatga gagattaaat
     3721 tttgaggaga gtggtgctgt agcgcaccgg gccggagcag acgattattc cggtggttta
     3781 cgttttcgtt tcggatgttg gataaacagc cagataaacg taaccacagc tgccgagggt
     3841 gtcggcttcg caggtcagcc ctctggcata cagcgtgacg gtatgctgat ggcgggggct
     3901 gagttcaccg ctggtgagca tgagttccag ttgtttcatc agcagcggaa aggcctggtc
     3961 cagatggtac gcatccgcgt cgctgaaccg gcctctgata ccggcgcggt cggcaaggta
     4021 atgcaaccgg ttaccctcct gcaccagacg tgccccaaaa cagggcgtca cggtgcaggg
     4081 tagcccccac caggggcggt cgttgttatc gtcgggataa tttgtttcat ggagtgagtc
     4141 tgacacgata aaatccttac agaaaatcgg tgaatgatag tttaacgatg acgagtcagc
     4201 cggcgcgcag accggcttcg gtgaaggtgt attcgttgat gagcagggcc tcatccacag
     4261 cggcgtctga ggtcagccag tcgtactcgt tttccagctg acggtacaac cagaaggcca
     4321 ggtcgcgcag gacttcgctg actgcggctt ccgcatcggt tgtcattacc tgaccagtcg
     4381 gactgttccg ggtgacggtg atatccatgc tgtacggatg gcagtaacgt ccccggtgac
     4441 tgatttcagc ctgtaactgc cagaagttac gcttctgtac cgcctgcagg gcatccgcga
     4501 tacgatgaag ttcacggtcc tgcggggcat actccctgat tttccggggt gccgctggct
     4561 gccagtgcca gagcccttca aaacaggctc cgtccccctg gctgcagaat ccgctgaacc
     4621 agatgcacgg ctcttccata aacctgccgt tgctgagccg gataacacgc tggcggaggt
     4681 caatcccgaa aatgtcacag acctccctga agtcttcata cacgttttca taccagttgc
     4741 tgtcagcatg atgctgacgg tactcgtcgc gggctttttc ccgtgcggtg gaggatagct
     4801 cattcagtgt atacacggtg gtggtgataa ttctgctcat ctgaatgccc gtcctgattc
     4861 agttcagggt gacgctcatc agccggacat acgggccaaa actgtcctta cggcgttcag
     4921 caaacacggc cagcacaccg ggaatatcct gcacttcacg accggtatac gcttcagcac
     4981 tgccgtgcca gcggtatttg ccggtacaga acggaaaaag acgggatgcc ggatgctgac
     5041 ggtgaatacg catggcttcg ccacgggtga tgattttcat agtgggatac ctctgaagac
     5101 agaagataaa agtgaaaaca ggtgtgatat ggttgtggcg gtgacgggtt aaagcagacc
     5161 gtgttccgca aaggagaaaa cctggttgcc accaactatc agatggtccg gtacccggat
     5221 atccaccagg gccagtgcct gtaccaggcg ttccgtgata aggcgatctg ccttactggg
     5281 agtgacttca ccggacgggt ggttgtgtgc cagtatcacg gcagcggcat tgtggtacag
     5341 ggcacgttta atcacttccc ggggatggac ttccgtgcgg ttgatggtgc cggtgaagag
     5401 ggtttcaccg gcaatcagct gattctggtt gttcagatac agtacccgga actcttcccg
     5461 ctccagtccc gccatgttca gaatcagcca ttcccgtgcc gcatgcgtgg aggtgaaggc
     5521 cacaccgggt tcatgaagat ggcggtccag ggttttcagg gcccgcagaa tgaggctgcg
     5581 ctcgccgggc gtcatctctc cgggcagaaa ggaaagctgt tgcattgtgc ttctctccat
     5641 tcagtcgatg atgcgcataa tggcgctgca ttccggatgc tgcagggcgt aatcccgcag
     5701 ccggtaatag tggaccgtca tggcgtaaca ctccgtacga caggcgtgat gactgtacgc
     5761 catcagacag gcggcaatgc cggcggcttc cgggctcatt tcagcgcggt taccgttcat
     5821 gacgttaaac agcacccatg tttcgtcatc atcgtcatcc ggttcgggtg ccataaatgc
     5881 gccaccgttg ttcagggtgt acagattcca gataccaccg cagtagtctt cacacagacg
     5941 gtccatccag ccgaagacac ggggctccag ggtcacccat tgtggaatga gaccaaaatg
     6001 ctgcggccag aagctgatgc gctgttcatc agggacggga gtggcaacca gctgaggcgg
     6061 gttattccct gatgcagcgg tcacggaaac agaaggtgtg gtggaattat gcgaagaggt
     6121 tgtcatgata ttattcctta taaaaagcaa atgaatgaag aaggctcggg ggcagggaca
     6181 gacgtgattc aggtaaacgg catcagcgca ggttctccag cagcgtttcc gccatcaccc
     6241 acaatgcgcg attgagctta atgtcggtgt cgatgttgtg aatggcccgg gtatggatac
     6301 gttttccttt tgcactgcgg ccggaaatcc cgcctttcag catattctcc tggatggtct
     6361 gataagcact ccacaggtcc ttaccgtaat cctcccggcg tcgtggcgtc agaatgtcgg
     6421 cagtggtgac tggctgatgt ttgtcaccat aacggtaagt cagtgccgcc tgtgccagcg
     6481 cctggcgtgc cggtggcggc aggaccagcg actgcatggc atcacgcttt tcctcaatcc
     6541 ggtcaaacac gcccaccacc tcgtaagccc cttcaatgac tctgtccacc acatttcccc
     6601 ggtgcggaac acgcacttcc cccagagact gaccacagac gcagccgttc tggcagacga
     6661 acctgaagta acccggcagc atctggtagc tggaggtacc gtcatgggag ttcagcagga
     6721 tgatttcagg gacatgttgt ccgtttatct ccccgtcacg gcgcagacgc agcatgtgtt
     6781 tggtataccc ccgacggctc tggtcgcgca cacgggtctg gcaggcgaag aatggctgaa
     6841 agccttcccg ctgcagattt tccaggacgg tgatggtggg aatgtacgca taccgttcac
     6901 tgcgggaggt gtgccggtct tctccaaaaa tactcgggac gtggtacatc agttcttcgc
     6961 gtgtcagtgg gcgctcccga cggatctgat ttacatgacc aaaacgactg gctaattgca
     7021 tactttgctc cttatgaatg aattagcggt ataaacgaaa aggccgcgtc tcccggagaa
     7081 gacgcggcct gatggatgaa atgaagacgt ttattgcctg agaattccct cactggcgag
     7141 atacgaattt agctgttttc cggcatcagg ttcaaaattc cagcaccgcc agcgtagctg
     7201 cccttcggca ttgcggataa ccagccggaa atgcgttccc tggtcatctt caataagcac
     7261 attgcggtaa gctgttgtga cagccatcgc ctgttcgcgt gagaaagggc cttcgggcaa
     7321 taaaacaggg tgacagtccg acatatcggc tattttccga taaattcaac gctggctccg
     7381 gtggcattac cgtcagcacc aaccgtgacg atcactttgt cgtacgcacg gtcttcagga
     7441 ttgaacactt caaactcaac cttattgcct tttacttcgt aagagccaaa atcagcggtt
     7501 tctgttgttt cctcgtcgta tttacgggcg tatgcctgtc gggcagcctg cagtacggag
     7561 tctttattaa tagtggtcgt ctgtttcggg gaaaaaccgg caagtgatgt gacaaatttc
     7621 ggacagacct tcagcatatc accgcgggtg aattccattc gtacaccaaa tgcctgtaaa
     7681 acggacacat cacgctctgc tatggagtga acggcttttt tgaccatttc cggcgcctgt
     7741 ccgatttcca cctcaacagc cctgaatccc gtctgctgat tacacatttg tgatgtgggg
     7801 atcaggttac cgttaatact gatacggctg cccaggagcg tctgtacacc attcgggcat
     7861 ccctgcatca ggccggtaat accaatcgtg ccatttttca gcattaccag cgcctgacca
     7921 tcttccgtca tggtctgaca ccagccgttt ttgacagccc agttgccggc ggaggcggag
     7981 aatgatgcca cagtgaacag caggggaaaa agaattctgg tattcatgac tgacctctga
     8041 aattaaagag cgtgttattg ataatgacag acggtatacg gcagtggtta ccgtaatccg
     8101 ttgtcgtgta aaggtatacg gttgccacgg gggactccgg ggcgctcaga acaggtgatc
     8161 ccacagtgtg accagcaggt ggataatgcg gtcccgggtt ttgcgcagaa gctgtaatat
     8221 ccaggctgga aagctgaacc gggtaatggc agcgtcaaag ctttttccga tggcgtcacc
     8281 aaaagtctgt tgtgcgtgtt tccgggcctg ttcagagcgg ttttcccccc ggatatgtga
     8341 ataaaccgcg ctggttgcct gcggtggcag tgcgtggatc atcagcgaca caaatgccgg
     8401 aaggttccag ccgacaggtg cggcaacggg gagtaccgga aatgatgaag ggaacagggt
     8461 tgccacccgg gctgttactg ttgccagtga gagttcctgc tgacgggacg ggcatttttc
     8521 tgtgtcgttc cattcttcag caggaaatac gcgatcggca tggctgagaa caaacagaaa
     8581 acgcgaggga tctgcacctt cattcagtaa aaactgatgc atggcaatat cggcagcata
     8641 cgcacgttca tcagcccgca ggatccagat aatcagatcc agttccggca gtaactgacg
     8701 gtaaagctcc cggtattcct gatcatgctg cggtgtttca ccaatgccgg gcagatcgac
     8761 cagcgtcatt ctgcgttcac cgagctgcag ggtaagacga tgagcctggc gggtgcagcc
     8821 gttcagggga tgcgtggcgc agatacggga ctgaaaaatg gcattacaca ggctgctttt
     8881 gccggtgccg gatttaccca taatgccaat cacgggctcg taatgaataa caccgtggag
     8941 atgttcaagg attttctggc gtatttcttc aggaagaaac gtcagatatg atttcacttg
     9001 tgcagagagg gaagaatggc tgaatgacat accggtatcc tgttgatttc actgaaccgg
     9061 atcacactaa tccggtttat cattcaggta atatggacgt gaaaaatgat ggaatgtctg
     9121 aattacggac atgattgtta aagtgtaatg gagtatgaag tgagtttttc cgaacgggaa
     9181 ctaatgacat actgcggttt caggagatga gaggatgacc ggacgtaaaa acaaatgtgt
     9241 cagaaaaaat tgtaacaatc aggttttctc agtaaaaatg gcagtggaga aaagactgtg
     9301 cagaaaacat tatgagaggt tttgtgagct caaaaaaaga agagagcaaa atcctgttca
     9361 cagattaaat gaa
//
LOCUS       DAZGLJ010000176         9217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000176 DAZGLJ010000000
VERSION     DAZGLJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.176"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..931
                     /gene="ldhA"
                     /locus_tag="ACHK49_003733"
     CDS             <1..931
                     /gene="ldhA"
                     /locus_tag="ACHK49_003733"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO9264302.1"
                     /translation="ESFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSRPVLEEL
                     KKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRRIHR
                     AYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAMLRILKGFGMRLLAFDPYPSAA
                     ALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEAAFDQMKNGVMIVNTSRGALI
                     DSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQ
                     AFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1042..1464
                     /gene="hslJ"
                     /locus_tag="ACHK49_003734"
     CDS             1042..1464
                     /gene="hslJ"
                     /locus_tag="ACHK49_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO9264303.1"
                     /translation="MKKLAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1461..1727)
                     /gene="ydbJ"
                     /locus_tag="ACHK49_003735"
     CDS             complement(1461..1727)
                     /gene="ydbJ"
                     /locus_tag="ACHK49_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO9264304.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2001..5525
                     /gene="nifJ"
                     /locus_tag="ACHK49_003736"
     CDS             2001..5525
                     /gene="nifJ"
                     /locus_tag="ACHK49_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO9264305.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGSWPMGTTRWEKRNIAEEIP
                     TWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            5892..7025
                     /gene="ompN"
                     /locus_tag="ACHK49_003737"
     CDS             5892..7025
                     /gene="ompN"
                     /locus_tag="ACHK49_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO9264306.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            7166..7600
                     /gene="uspF"
                     /locus_tag="ACHK49_003738"
     CDS             7166..7600
                     /gene="uspF"
                     /locus_tag="ACHK49_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO9264307.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(8388..8510)
                     /locus_tag="ACHK49_003739"
                     /pseudo
     CDS             complement(8388..8510)
                     /locus_tag="ACHK49_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(8541..9182)
                     /locus_tag="ACHK49_003740"
     CDS             complement(8541..9182)
                     /locus_tag="ACHK49_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO9264308.1"
                     /translation="MPVDLTPYILPGVSFLSDIPQETLSEIRNQTIRGEAQIRLGELM
                     VSIRPMQVNGYFMGSLNQDGLSNDNIQIGLQYIEHIERTLNHGSLTSREVTVLREIEM
                     LENMDLLSNYQLEELLDKIEVCAFNVEHAQLQVPESLRTCPVTLCEPEDGVFMRNSMN
                     SNVCMLYDKMALIHLVKTRAAHPLSRESIAVSMIVGRDNCAFDPDRGNFVLKN"
BASE COUNT         2443 a         2258 c         2156 g         2360 t
ORIGIN      
        1 cgagtccttt ggctttgagc tggaattttt tgactttctg ctgacggaaa aaaccgctaa
       61 aactgccaat ggctgcgaag cggtatgtat tttcgtaaac gatgacggca gccgcccggt
      121 gctggaagag ctgaaaaagc acggcgttaa atatatcgcc ctgcgctgtg ccggtttcaa
      181 taacgtcgac cttgacgcgg caaaagaact ggggctgaaa gtagtccgtg ttccagccta
      241 tgatccagag gccgttgctg aacacgccat cggtatgatg atgacgctga accgccgtat
      301 tcaccgcgcg tatcagcgta cccgtgacgc taacttctct ctggaaggtc tgaccggatt
      361 tactatgtat ggcaaaacgg caggcgttat cggtaccggt aaaatcggtg tggcgatgct
      421 gcgcattctg aaaggttttg gtatgcgtct gctggcgttc gatccgtatc caagtgcagc
      481 ggcgctggaa ctcggtgtgg agtatgtcga tctgccaacc ctgttctctg aatcagacgt
      541 tatctctctg cactgcccgc tgacaccgga aaactaccat ctgttgaacg aagccgcctt
      601 cgatcagatg aaaaatggcg tgatgatcgt caataccagt cgcggtgcat tgattgattc
      661 tcaggcagca attgaagcgc tgaaaaatca gaaaattggt tcgttgggta tggacgtgta
      721 tgagaacgaa cgcgatctgt tctttgaaga caaatccaac gacgtgatcc aggatgacgt
      781 gttccgtcgt ctgtctgcct gccacaacgt gctgtttacc gggcaccagg cattcctgac
      841 agcagaagct ctgaccagta tttctcagac tacgctgcaa aacttaagca atctggaaaa
      901 aggcgaaacc tgcccgaacg aactggttta atcttgccgc tcccctgcaa tccaggggag
      961 ctgattcaga taatccccaa tgacctttca ttctctattc ttaaaatagt cctgagtcag
     1021 aaactgtaat tgagaaccac aatgaagaaa ctagccgcgt ttgttgcgct aagcctgctg
     1081 atggcgggat gtgtaagtaa tgacaaaatt gctgttacgc cagaacagct acagcatcat
     1141 cgctttgtgc tggaaagcgt aaacggtaag cccgtgacca gcgataaaaa tccgccagaa
     1201 atcagctttg gtgaaaaaat gatgatttcc ggcagcatgt gtaaccgctt tagcggtgaa
     1261 ggcaaactgt ctaatggtga actgacagcc aaagggctgg caatgacccg tatgatgtgc
     1321 gctaacccgc agcttaatga actcgataac accattagcg aaatgctgaa agaaggtgca
     1381 caagtggatc tgaccgcgaa ccagttaacg ctggcgaccg cgaaacagac attaacttat
     1441 aagctggcgg atttaatgaa ttaatagctg ccacagctcc cggcggcaag tgactgttcg
     1501 ctacagcgtt tgccgttggg taatgcacac atcccaatcg ccgtaccatc cagttgacgg
     1561 gcaacagaaa gcgaaccgcc gatcattgca caatttgctt ctccactact ggacatcgac
     1621 gcttttaaac ctggcgctac gtgcgccgca gtcgcctgct gaacaggttc actactacac
     1681 gccgacaaca ataaagcggc acaccctacc caaaacgctg ctcgcatctc ttcttcctct
     1741 gatcttcaag ccaaacgaca ccgccataaa taataggcag cacagagggc ggcgtcgaga
     1801 gctgtcctgc gcgttgcccc gcatttttac ttttttatgg ctattttttt gccctctgtt
     1861 tgatcaaaac attcattacg ctgatgtggg ggacacaaaa gcgaaaatgc agaagaaagc
     1921 catttgctaa aattgaagga ttactactgg gcgcgcagca atttcgtgcg cccctcattt
     1981 gcgcaatgta agggtgtcat atgattacta ttgacggtaa tggcgcggtt gcttcggtgg
     2041 cgtttcgcac cagtgaagtt atcgccatct accctattac ccccagttcc acgatggcag
     2101 aacaggctga tgcctgggcc ggaaacggct tgaagaacgt ttggggagac acaccacgcg
     2161 tggttgaaat gcagtcggaa gcgggtgcta tcgctaccgt gcatggcgct ttgcagacgg
     2221 gtgccctttc aacatcgttt acgtcatcgc agggtttgct gctgatgatc ccgacgctgt
     2281 acaaactggc aggcgaactc acaccgtttg tcctgcatgt agcggcacgt accgttgcca
     2341 cacatgcact ctctattttt ggcgatcatt ccgacgtcat ggcggtgcgc cagacgggtt
     2401 gcgcgatgtt gtgtgcggca aacgtccagg aagcgcaaga ctttgctctc atttcgcaca
     2461 tcgcgacgct gaaaagccgc gtgccattta ttcatttctt tgatggtttc cgcacgtccc
     2521 acgaaatcaa caaaattgtc ccgctggccg atgacacgat tcttgatctc atgccgcagg
     2581 ccgaaattga tgctcatcgc gcccgggcac tcaacccgga acatccggtg atccgcggta
     2641 cgtccgccaa tcctgacact tatttccagt ctcgcgaagc caccaaccca tggtacgacg
     2701 cggtctatga ccatgttgaa caggcgatga atgatttctc tgccgcgaca ggtcgtcagt
     2761 atcagccgtt tgaatattac gggcatccgc aagcggaacg ggtgattatc ctgatgggct
     2821 ctgccattgg cacctgtgaa gaagtggttg atgaattgct aacccgtggc gaaaaagtcg
     2881 gcgtgctgaa agttcgcctg taccgcccct tctcagccaa acacttgcta caaactctac
     2941 cgggatccgt acgtagcgtg gcggtactgg acagaaccaa agaacccggt gcccaggcag
     3001 aaccgctcta tctggatgtg atgaccgcac tggcagaagc ctttaataat ggcaagcgcg
     3061 aaactctccc ccgtgtcatt ggtgggcgct atggtctttc atccaaagaa tttggcccgg
     3121 actgtgtact ggcggtattt gccgagctca acgcggctaa accgaaagcg cgctttacgg
     3181 ttggtattta cgatgatgta accaatctgt ccctgccgtt gccggaaaac acccagccaa
     3241 actcggcgaa acttgaagcc ttgttttatg gccttggtag tgatggcagc gtttccgcga
     3301 ccaaaaacaa tatcaagatt atcggtaatt ccacgccgtg gtacgcacag ggctattttg
     3361 tttacgactc caaaaaggcg ggcggcctga cggtttctca ccttcgagtg agcgaacagc
     3421 cgattcgttc cgcttatctc atttcccagg ctgattttgt tggctgccac cagttgcagt
     3481 ttatcgataa atatcagatg gctgagcgtt taaaacctgg cggcattttc ctgctcaaca
     3541 caccgtacag cgcagatgaa gtgtggtcgc gcttgccgca agaagttcag gccgtgttaa
     3601 accagaaaaa agcgcgcttc tatgtgatta acgcggcgaa aatcgcccgc gaatgtggcc
     3661 tggcggcccg tattaatacc gtcatgcaga tggctttttt ccatctgacg caaattctgc
     3721 ctggcgatag cgccctcgca gaattgcagg gtgcgattgc caaaagttac agtagcaaag
     3781 gccaggatct ggtggaacgc aattggcagg ctctggcgct ggcgcgtgaa tccgtagaag
     3841 aagttccgtt gcaaccggta aatccgcaca gcgccaatcg accgccagtg gtttccgatg
     3901 ccgctcctga tttcgtgaaa accgtaaccg ctgcgatgct cgccgggctt ggtgacgccc
     3961 tccccgtttc ggcgctgccg ccagacggct cctggccgat gggcactacg cgctgggaaa
     4021 aacgtaatat tgccgaagag atccccacct ggaaagagga actctgtacc caatgtaacc
     4081 actgcgttgc cgcttgccca cactcagcta ttcgcgcaaa agtggtgccg cctgaagcga
     4141 tggaaaacgc ccctgccagc ctgcattcgc tggatgtgaa atcgcgtgat atgcgcgggc
     4201 agaaatatgt cttgcaggtg gcaccggaag attgcaccgg ttgtaacctg tgcgtcgaag
     4261 tttgcccggc gaaagaccgt cagaatccag agattaaagc catcaatatg atgtctcgcc
     4321 tggaacatgt cgaagaagag aaaatcaatt acgatttctt cctcaacctg ccagaaatcg
     4381 accgtagcaa actggaacgt attgatattc gtacatcgca gctgattaca ccgctgtttg
     4441 aatattcagg tgcttgctcc ggttgtggcg agacgccgta tattaaattg ctgactcagc
     4501 tctatggcga ccggatgttg atcgctaacg ccaccggctg ttcttcaatt tatggcggta
     4561 acctgccctc tacaccgtat accaccgatg ccaacggtcg tgggccggca tgggcgaact
     4621 ctctatttga agataatgcc gaatttggcc ttggtttccg cctgacggtc gatcaacacc
     4681 gtgtccgcgt gctgcgtctg ctggatcaat ttgccgataa aatcccggcg gaattactga
     4741 cggcgttgaa atcagacgct acgccagagg ttcgtcgaga acaggtcgca gctttacgcc
     4801 agcaactcaa cgatgttgcc gaagcacatg aactgctgcg tgacgcagat gcactggtgg
     4861 aaaaatcaat ctggctgatt ggtggcgacg gctgggctta cgatatcggc tttggcggtc
     4921 tggatcatgt attgagtttg acggaaaacg tcaacattct ggtgctggat acgcaatgct
     4981 attccaacac cggtggtcag gcgtcgaaag cgacaccgct gggtgcagta actaagttcg
     5041 gcgagcacgg caaacgtaaa gcgcgtaaag atcttggcgt cagtatgatg atgtacggtc
     5101 atgtttatgt ggcgcagatt tctctcggcg cgcagctgaa ccagacggtg aaagcgattc
     5161 aggaagcgga agcgtatccg gggccatcgc tgatcattgc ttatagcccg tgtgaagagc
     5221 atggttacga tctggcactc agccatgacc agatgcgcca actcacagct accggcttct
     5281 ggccgctata tcgcttcgat cctcgtcgtg ccgatgaagg caaactgccg ctggcgctgg
     5341 attcacgccc gccgtcagaa gcactggaag aaacgttact tcacgagcaa cgtttccgtc
     5401 gtctgaattc gcagcagcca gaagtggcag aacagttatg gaaagatgct gcagctgatt
     5461 tgcaaaaacg ctatgacttc ctggcacaaa tggccggaaa agcggaaaaa agcaacaccg
     5521 attaaatgct ctggataagg attatccaat tctaaaaaaa aagcccggac gactgttcgg
     5581 gcttgtcttt ttatatgttg gaaaatcagt ggcaatgcaa tggcccaaca gaaaataaaa
     5641 tctgtgacaa taaaggcata taacccgcgc agaataacgt atagaaaatt aaaagtataa
     5701 tttttatttt gtatgtatta tcttcgatac attcaccaga cttattatca tttcaggaga
     5761 attatttgat taaggtttta cttaaggcgt aacaaatgat atttatcggc taactgaact
     5821 tctcctttag gatgttttca ctccccaaaa tggggataaa aggcaaataa aataactaaa
     5881 ggatttattc aatgaaaagc aaagtactgg cacttttaat tcctgccctg ctcgccgcag
     5941 gtgctgcaca tgcagccgaa gtttataata aagacggcaa caaattagat ctgtatggca
     6001 aagttgatgg cctgcattat ttttctgata attcagcgaa agatggcgac cagagctatg
     6061 cgcgtctggg ttttaaaggc gaaacccaaa ttaacgatca acttactggc tacggtcaat
     6121 gggaatacaa tattcaggca aacaacactg aatcttcaaa aaaccagtca tggacccgtc
     6181 tggcatttgc cggactgaaa tttgcagatt acggttcttt cgattacgga cgtaattatg
     6241 gcgtaatgta cgacatcgaa ggctggaccg atatgctgcc tgaatttggc ggtgactctt
     6301 ataccaatgc agacaacttt atgactggtc gagccaatgg cgtcgcgact tatcgtaata
     6361 ctgatttctt cggtctggta aatggtctga acttcgcggt gcagtatcaa ggtaacaacg
     6421 aaggtgccag taatggtcag gaaggcacca acaacggacg tgatgttcgc catgaaaacg
     6481 gtgacggctg gggtctttcc acaacatatg atttaggcat gggctttagc gctggtgcgg
     6541 catacacctc ttctgaccgc accaatgacc aggttaacca tactgcggcg ggtggtgata
     6601 aagcagacgc gtggactgct gggctaaaat acgatgctaa caatatttac ctggcaacca
     6661 tgtattcaga aacgcgtaat atgaccccgt ttggcgacag cgattatgct gttgcaaaca
     6721 aaacccagaa ctttgaagtc actgcacagt accagtttga ttttggtctg cgtccggcag
     6781 tctctttcct gatgtctaaa ggccgtgacc tgcacgctgc cggtggtgca gacaacccgg
     6841 caggtgttga tgataaagat ctggttaaat acgccgatgt tggcgcgact tactatttca
     6901 ataaaaacat gtccacttat gttgactata aaatcaacct gttggatgaa gatgacagtt
     6961 tctacgctgc caacggcatc tctaccgatg atattgtcgc tttaggtctg gtttatcagt
     7021 tctaaatcct cctgcccgct gttatggcgg gctttttctg cttattcttc ctcctttgat
     7081 ctaaattaaa aatgtgaact ccgtcattac acaaaaagtg tcatctggcg ttacacttta
     7141 tgcggatact aaaacaggag gttttatgaa cagaacgatt cttgtcccta tcgatatttc
     7201 cgattcagaa ttaactcaac gcgtgattag ccacgttgag gcagaggcaa agattgatga
     7261 tgcagaggtt catttcctga cggtaatacc ttcactgccc tactatgcct ctctgggttt
     7321 agcgtattcc gcagaattac cggcaatgga tgacctgaaa gcggaagcca aatcgcaact
     7381 ggaagagatc attaaaaaat ttaaactgcc aaccgacaga gtgcatgtcc atgttgagga
     7441 aggctcgcca aaagaccgca ttctggaatt ggcgaagaag atccccgctc atatgatcat
     7501 cattgcttcc catcgaccgg atatcaccac ttatctgctc ggttctaacg ccgcagctgt
     7561 agtgcgtcac gcagagtgct ccgtgctggt tgtgcgctga cactaacgcc cgcacattgc
     7621 tgcgggcttt ttgattcatt tcgcaaatgt gctgacattt tcccctctaa tccgtaccat
     7681 acacgccaca gtttttatat cagatttctt atgctgggcg ttccggcatg acgcatactc
     7741 ttctgatgcc atataacgaa ttgagtcgct tttaaatgtc gcaaaatcaa gaaattagta
     7801 agaaagaaca atacaacctg aacaagtcag agtaaaaacc gcaactatct gacgaacaat
     7861 aacttttatg tttttcaata tgttaggcag gattaacaac tacaataaag cacaataatc
     7921 tacacttttt ttgccccatt tttgcccctt ttgcggcctt ttgtcccatt tttgccacag
     7981 taaaaatcta taactaatca atctgtgatc tgtaaattgc gcccatgatt tctttccaat
     8041 agaatcagtt ccggctggaa atcattagat actcgcacta tcgaaaagct taccagccaa
     8101 ccgcggcacg ttcttgcata cgacgtggct acgggtttcg agaccgatga tacagagtcg
     8161 aaaaatcaaa gagttaaaaa tttttcttgg aataaatgtc ttaaaatcgt acacgaaaaa
     8221 tcaataaatt acataacatt taaagaagaa tattccaagc cttcttgagg ccaccacctg
     8281 ttttcaggtg tcattcagac gtgatttacc ccagattggg aaagcacgct ttttttctca
     8341 gcttattccg ttttgcctgt gtgttgaata attccactga agttatataa aatcggtaac
     8401 ggctggaaat catccaatac tagcactatc gaaagttcac caaccaaccg cagtacgttc
     8461 ttgcatacga ggtgccgcgt tttacatgcc gtaccgagaa ttaatttcat tttacgaaaa
     8521 gttaacccgc cattgctatc ttaatttttt aaaacgaagt tacctctgtc agggtcaaaa
     8581 gcacaattat ctcttcctac aatcattgaa actgcgattg attccctgct caaaggatga
     8641 gccgcccttg ttttaacaag atgtattaat gccattttat catacaacat acaaacattt
     8701 gaattcattg aattcctcat gaatacccca tcttctggtt cacataatgt aacagggcat
     8761 gttcgtaagc tctctggcac ttgcaattgt gcatgctcca cattaaatgc acatacttca
     8821 attttatcta acaactcctc taactggtag ttagaaagca aatccatatt ttcgagcatc
     8881 tcaatttcac gcagtactgt aacttcacgg cttgtcaaac taccatgatt aagtgtacgt
     8941 tcaatatgtt ctatatattg aaggccaatc tggatattat cattcgataa accatcctgg
     9001 ttaagacttc ccataaaata tccatttacc tgcataggtc gtattgacac catcaactca
     9061 cccagtctta tttgagcttc tccacgaata gtctgattac gtatctcaga caaggtttct
     9121 tgaggaatgt cagacaaaaa actaacccca ggtaaaatat aaggcgttaa atctacaggc
     9181 attcctctcc ctccatttta ataacttaat tttgctt
//
LOCUS       DAZGLJ010000177         9011 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000177 DAZGLJ010000000
VERSION     DAZGLJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.177"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            179..1237
                     /gene="citC"
                     /locus_tag="ACHK49_003741"
     CDS             179..1237
                     /gene="citC"
                     /locus_tag="ACHK49_003741"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO9264309.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1252..1548
                     /gene="citD"
                     /locus_tag="ACHK49_003742"
     CDS             1252..1548
                     /gene="citD"
                     /locus_tag="ACHK49_003742"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO9264310.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1545..2453
                     /gene="citE"
                     /locus_tag="ACHK49_003743"
     CDS             1545..2453
                     /gene="citE"
                     /locus_tag="ACHK49_003743"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO9264311.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2464..3996
                     /gene="citF"
                     /locus_tag="ACHK49_003744"
     CDS             2464..3996
                     /gene="citF"
                     /locus_tag="ACHK49_003744"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO9264312.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLATFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4000..4551
                     /gene="citX"
                     /locus_tag="ACHK49_003745"
     CDS             4000..4551
                     /gene="citX"
                     /locus_tag="ACHK49_003745"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO9264313.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4526..5404
                     /gene="citG"
                     /locus_tag="ACHK49_003746"
     CDS             4526..5404
                     /gene="citG"
                     /locus_tag="ACHK49_003746"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO9264314.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5455..6918
                     /gene="citT"
                     /locus_tag="ACHK49_003747"
     CDS             5455..6918
                     /gene="citT"
                     /locus_tag="ACHK49_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO9264315.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7032..7838
                     /gene="rna"
                     /locus_tag="ACHK49_003748"
     CDS             7032..7838
                     /gene="rna"
                     /locus_tag="ACHK49_003748"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO9264316.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8068..8478
                     /gene="rnk"
                     /locus_tag="ACHK49_003749"
     CDS             8068..8478
                     /gene="rnk"
                     /locus_tag="ACHK49_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO9264317.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8616..8705
                     /gene="yldA"
                     /locus_tag="ACHK49_003750"
     CDS             8616..8705
                     /gene="yldA"
                     /locus_tag="ACHK49_003750"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO9264318.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8709..>9011)
                     /locus_tag="ACHK49_003751"
     CDS             complement(8709..>9011)
                     /locus_tag="ACHK49_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264319.1"
                     /translation="FIHGFLFGDAFDKGLTFKMGQTHVHAWLGELLPLIEKGLLKPEE
                     IVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQSAEAAQKAVSGLVNAMPGGTI"
BASE COUNT         2079 a         2440 c         2316 g         2176 t
ORIGIN      
        1 gaaataaatc aaaatttcac gccgaaatac tccttaggat gtatagcgaa aggagaaaaa
       61 gatatacctc gatcaccccc ctttctccca agtgaaaata aaaggttatc agtttgcaac
      121 attgaacaac attcgttgca aatcgataac aacatgcacc ttcaggatac tatttattat
      181 gttcggcaat gatattttca cccgcgtaaa acgttcagaa aataaaaaaa tggcggaaat
      241 cgcccaattc ctgcatgaaa atgatttgag cgttgacacc acagtcgaag tatttattac
      301 cgtaacccgc gatgaaaagc ttatcgcgtg cggtggaatt gccggaaata ttattaaatg
      361 cgttgctatc agtgaatccg ttcgcggtga aggactggcg ctgacattag ccactgaact
      421 gataaacctc gcctatgagc ggcacagcac gcatctgttt atttatacca aaaccgaata
      481 cgaggcgctg ttccgccagt gcggtttttc cacgctgacc agcgtacccg gcgtgatggt
      541 gctgatggaa aacagcgcca cgcggctgaa acgctatgcc gaatcgctga aaaaatttcg
      601 tcatccaggg aacaagattg gctgcattgt gatgaacgcc aatcccttca cgaatggtca
      661 ccgttatctg attcaacaag ctgcggcaca gtgcgactgg ttgcatctgt ttttagtcaa
      721 agaagattct tcacgcttcc cctatgaaga ccggttggat ctggtgttaa aaggcaccgc
      781 cgatattcca cgcctgactg tgcatcgcgg ctccgaatac atcatctccc gcgctacgtt
      841 cccttgctac ttcattaaag aacagagcgt cattaaccat tgttacaccg aaattgatct
      901 gaagattttc cgtcagtacc tcgctcccgc gctgggtgta actcaccgct ttgtcggtac
      961 tgaacccttt tgtcgcgtta ccgcccagta caaccaggat atgcgctact ggctggaaac
     1021 gccgactatc tccgcaccgc ccatcgaact ggttgaaatt gagcggctgc gttaccagga
     1081 gatgccgata tccgcttccc gggtacgtca actgctggcg aaaaacgatc tcacggctat
     1141 cgcgccgctg gtccctgcag tcacgctgca ttatttgcag aacctgcttg agcactcccg
     1201 ccaggacgcg gcagctcgtc aaaagacccc cgcatgagaa acaggtgaaa aatgaaaata
     1261 aaccagcccg ccgttgcagg cacccttgag tctggggatg tgatgatacg catcgcccca
     1321 ctcgatacgc aggatatcga cctgcaaatc aatagcagcg ttgagaaaca gtttggcgat
     1381 gcaattcgca ccaccattct ggacgttctc gcccgctaca acgtgcgcgg cgtacagctg
     1441 aatgtcgatg acaaaggcgc actggactgc attttacgtg cacgactgga agccctgctg
     1501 gcacgcgcca gcggtatccc ggctctgcca tgggaggatt gccaatgatt tccgcttcgc
     1561 tgcaacaacg taaaactcgc acccgccgca gcatgttgtt tgtgcctggt gccaatgccg
     1621 cgatggtcag caactccttc atctacccgg ctgatgccct gatgtttgac ctcgaagact
     1681 ccgtggcatt acgtgaaaaa gacaccgccc gccgcatggt ttatcacgcg ctgcaacatc
     1741 cgctgtatcg cgatattgaa accattgtgc gtgtcaacgc gctggattcc gaatggggtg
     1801 ttaacgacct ggaagccgtc gttcgcggtg gtgcggacgt tgtgcgtctg ccgaaaaccg
     1861 ataccgctca ggatgttctg gatattgaaa aagagatcct gcgtatcgaa aaagcctgtg
     1921 gtcgtgaacc cggcagcacc ggcctgctgg cggcgattga atctccgctg ggcattaccc
     1981 gcgcagtgga aatcgctcac gcttccgagc gtttgatcgg tatcgccctc ggtgcagaag
     2041 actatgtgcg caacctgcgt acagaacgct ccccggaagg aactgaactg ctgttcgcac
     2101 gctgttccat tttgcaggcc gcgcgctctg cgggtattca ggcgttcgat accgtctatt
     2161 ccgacgctaa caacgaagcc ggattcctgc aagaagccgc ccacatcaaa cagctgggct
     2221 ttgacggcaa atcgctgatc aacccgcgtc agattgatct gttgcacaac ctctacgcac
     2281 caacccagaa agaagtggat cacgcccgcc gcgtcgtaga agccgctgaa gccgccgctc
     2341 gcgaaggcct cggcgtggtt tccctgaacg gcaagatggt ggacggtccg gttatcgatc
     2401 gcgcccgtct ggtgctctct cgtgcagaac tttccggcat ccgcgaagaa taaggcaatc
     2461 aaaatgacgc agaaaattga acaatctcaa cgacaagaac gggtagcggc ctggaatcgt
     2521 cgcgctgaat gcgatcttgc cactttccag aactcgccaa agcaaaccta ccaggctgaa
     2581 aaagcgcgcg atcgcaaact gtgcgccaac ctggaagaag cgattcgtcg ctctggttta
     2641 caggacggca tgacggtttc cttccatcac gctttccgtg gcggtgacct gaccgtcaat
     2701 atggtgatgg acgtcatcgc gaagatgggc tttaaaaacc tgaccctggc gtccagctcc
     2761 ctgagtgatt gccatgcgcc gttggtagaa cacattcgcc agggcgtggt tacccgcatt
     2821 tatacctccg gcctgcgcgg tccactggcg gaagagatct cccgtggtct gctggcagaa
     2881 ccggtgcaaa tccactctca cggcggtcgc gtgcatctgg tacagagcgg cgaactgaat
     2941 atcgacgtgg ctttcctcgg cgtcccgtcc tgcgatgaat tcggtaatgc caacggctac
     3001 accggtaaag cctgctgcgg ctccctcggc tatgcaatgg ttgatgccga caacgcaaag
     3061 caggtcgtga tgcttaccga agaactgctg ccttatccgc ataatccggc aagcattgag
     3121 caagatcagg ttgatttgat cgtcaaagtt gaccgcgttg gcgatgctgc aaaaatcggc
     3181 gctggcgcga cccgtatgac cactaacccg cgcgaactgc ttattgcccg tagcgctgcg
     3241 gatgtgattg tcaactcagg ctacttcaaa gaaggtttct ccatgcaaac cggcaccggc
     3301 ggcgcatcgc tggcggtaac ccgtttcctg gaagacaaaa tgcgtagccg cgatattcgc
     3361 gccgacttcg cccttggcgg tattaccgcg acgatggttg acctgcacga aaaaggtctg
     3421 atccgcaaac tgctggatgt gcagagcttt gacagccatg ctgcgcaatc gctggcccgt
     3481 aaccccaatc acatcgaaat cagcgccaac cagtacgcta actggggttc gaaaggcgca
     3541 tcggttgatc gtctcgacgt ggtggtactg agcgcgctgg aaattgacac ccagttcaac
     3601 gttaacgtgc tgaccggctc tgacggcgta ctgcgtggtg cttccggtgg tcactgcgat
     3661 accgcgattg cctctgcgct ttccatcatc gtcgcgccgc tggtacgcgg tcgtattccg
     3721 actctggtgg ataacgtgct gacctgcatc accccgggct ccagtgtcga tattctggtc
     3781 acagaccacg gtatcgcagt taacccggca cgtccggaac tggcagaacg tctgcaggaa
     3841 gcgggcatta aagtggtttc cattgagtgg ctgcgcgaac gtgcgcgtct gctgaccggt
     3901 gaaccacagc cgattgaatt caccgaccgc gtcgttgccg ttgtgcgtta ccgcgatggc
     3961 tcggtgatcg atgttgtgca tcaggtgaag gaataagcca tgcacctgct tcctgaactc
     4021 gccagccacc atgcggtgtc aattcccgag ctgctcgtca gccgggatga aaggcaagca
     4081 cggcaacacg tctggctcaa gcgccatcct gttccactgg tctcctttac cgtggttgcg
     4141 cctgggccga ttaaagacag cgaggtcaca cgccgaattt ttaatcatgg cgtgacagcc
     4201 ttgcgtgcct tagccgcaaa acagggctgg caaattcagg agcaggctgc actggtttcc
     4261 gccagcgggc cggagggcat gttgagcatt gccgccccgg ctcgcgacct caagctcgcc
     4321 accattgagc ttgaacatag tcatcctctc gggcggttat gggatatcga tgtcctgacg
     4381 cccgaaggcg aaattctctc ccgccgcgac tattcactgc cgcctcgccg ctgcctgttg
     4441 tgcgagcaaa gcgcagccgt ctgcgcgcgt ggaaaaaccc atcaactgac cgatttactc
     4501 aaccgcatgg aggcactgct gaacgatgtc gatgcctgca acgtcaacta aaaccacaaa
     4561 gcttgcgacg tcattaatcg atgagtacgc cctgctgggc tggcgcgcca tgctgactga
     4621 agtcaatctg tcaccgaaac caggcctcgt ggatcgcatt aactgcggtg cgcacaaaga
     4681 tatggcgctg gaagatttcc accgcagcgc gctggcgatt cagggctggt taccccgttt
     4741 cattgaattt ggtgcctgta gtgcagaaat ggcaccggaa gcggtactcc acggattacg
     4801 cccaattggt atggcttgcg aaggtgatat gttccgcgcc actgcgggcg taaacacgca
     4861 taaaggcagc attttttctt tagggctgct atgtgcggca attggccgtt tgcttcagct
     4921 caaccagccg gtaacgccaa caaccgtttg ttctacggcg gcaagtttct gccgtggtct
     4981 gaccgatcgc gaactgcgta ccaataattc acaactgacg gcaggtcaac ggttgtacca
     5041 gcagcttggc cttaccggcg cacgcggtga agccgaagcg ggttatccac tggtgatcaa
     5101 ccacgccttg ccgcattacc tcactctgct ggatcagggg ttagatcctg aactggcatt
     5161 gctcgatacc ttgctcctac tgatggcgat caacggcgat accaacgttg cttcgcgcgg
     5221 tggcgagggg ggcctgcgct ggctacagcg cgaggcgcaa acattattgc aaaaaggggg
     5281 cattcgaacc cccgccgatc tcgattatct ccggcagttc gacagggagt gtatcgaacg
     5341 aaatctcagt ccaggcggca gtgccgacct gctgatcctt acctggtttt tagcacagat
     5401 ttaattattt aagcacttga taaatttgaa aatattaatt ttcggagaac ccgtatgtct
     5461 ttagcaaaag ataatatatg gaaactattg gccccactgg tggtgatggg tgtcatgttt
     5521 cttatccctg tccccgacgg tatgccacca caggcatggc attacttcgc tgtgtttgtg
     5581 gcaatgattg tcggcatgat cctcgagcca attccggcaa cagcgatcag ttttattgcg
     5641 gttactattt gcgttattgg cagtaattac ctgctctttg atgccaaaga attagctgac
     5701 ccagcgttta atgcgcaaaa acaggcgctg aaatggggtc tggctggttt ttccagcacc
     5761 acggtatggc tggtatttgg cgcatttatt tttgcattag ggtatgaagt ttccgggtta
     5821 ggtcgtcgca ttgccctttt cctggtgaaa ttcatgggca aacgcacgct gacgttgggt
     5881 tatgcgattg tcattatcga cattctgctg gcaccgttta caccgtccaa caccgcgcgt
     5941 accgggggta cggtttttcc ggtcattaaa aacctgccgc cgttgtttaa atcattcccc
     6001 aacgatccgt ccgcgcgtcg tattggcggc tatttgatgt ggatgatggt cattagtacc
     6061 agtctgagtt cgtccatgtt tgtcaccggt gcggcaccaa acgtgctggg tctggagttc
     6121 gtcagcaaaa ttgccggtat ccagattagc tggttgcagt ggttcctctg cttcctgccg
     6181 gttggggtta tcttgcttat cattgcgccg tggctttcct acgtgctgta caaaccggaa
     6241 atcacacaca gtgaagaagt ggcaacctgg gcgggtgatg aactgaaaac catgggtgcg
     6301 ctgacacgca gagagtggac actgattggc cttgtattgc tcagcttagg tttgtgggtt
     6361 tttggcagcg aagtcattaa tgctactgca gttggtctgc tggcagtttc gctaatgctg
     6421 gccctgcacg ttgtgccttg gaaagatatt acccgctata acagcgcatg gaacacgctg
     6481 gtcaacctag caactctggt tgtgatggct aacggcctga ctcgttctgg ctttattgac
     6541 tggttcgccg gtaccatgag tacgcacctg gaaggattct caccaaacgc aacggtgatt
     6601 gtactggttc tggtgttcta ctttgcacac tacctgtttg ccagcctgtc tgcgcacacc
     6661 gcaaccatgc tgccggttat tctggccgtc ggtaaaggta ttccgggcgt accaatggaa
     6721 caactgtgta tcctgctggt gctgtctatc ggtatcatgg gctgtctgac gccgtatgca
     6781 accggtcctg gggtgattat ttacggctgt ggctatgtga aatcaaaaga ttactggcgt
     6841 cttggcgcaa tcttcggggt gatttacatc tctatgttgc tgttggttgg ctggccgatt
     6901 ctcgccatgt ggaactaata cgttcgccaa acgctgaata aatcatcacg cccgccaggt
     6961 aagcccacct ggcgggcttt tttatgattt aatagatagt attcagtgta tgtatgagtt
     7021 ccacacccat tatgaaagca ttctggcgta acgccgcgtt gctcgcggtt tctctgcttc
     7081 ccttctcttc tgccaacgcc ttagcgttgc aggcaaaaca gtatggcgat tttgatcgct
     7141 atgtcctggc cctctcctgg caaaccggat tttgccagag tcaacacgat cgaaatcgta
     7201 acgaacgaga tgaatgtcgc ctgcaaaccg aaacgaccaa caaagctgat tttctgaccg
     7261 tacatggtct gtggccagga ttgcctaaat cggttgctgc ccgtggtgtt gatgaacgcc
     7321 gctggatgcg cttcggttgc gctactcgcc caatcccgaa tctaccagaa gcgcacgcca
     7381 gccggatgtg ttcatcgccg gaaaccggat tatcactgga aacggccgct aaactaagtg
     7441 aagtcatgcc aggagctggc ggacgttcct gcctggaacg ctacgaatat gccaaacacg
     7501 gtgcctgctt tggttttgat ccggacgcat acttcggtac gatggtacgc ctgaatcaag
     7561 aaattaaaga gagcgaagcc ggaaaattcc ttgcggataa ttacggtaaa acagtgagcc
     7621 gccgtgactt tgacgccgcc tttgccaaaa gctggggaaa agagaacgtg aaagcagtta
     7681 agctaacgtg ccagggtaac cctgcgtatt tgactgaaat tcagatctcg atcaaagctg
     7741 acgcaatcaa cgctccgctt tctgcaaact cattcttgcc acaaccacac ccaggcaact
     7801 gtggcaaaac ctttgtgatt gataaagtgg gttattaatc atctgattta caaaacacgc
     7861 taattcgtct tccataaacc gtgacgatta agaattatac ataatgcgct gcattgagga
     7921 attccctgaa tgtgacgcaa atcacttcaa agagtgagca acagtcagta tcatcatgtg
     7981 agttaaaccc tcgccgcctg acggtgaggg ttttcttttg ggtatgttta tctgcgacac
     8041 tcgcagtacc gacgacatgg agtaaaaatg tccagaccaa ctatcatcat taacgacctg
     8101 gatgccgaac gcatcgatat tctgctggag caacccgcct atgctggttt gccaatcgcc
     8161 gacgcgttaa acgcagagtt ggatcgcgcc caaatgtgtt cgccagaaga gatgccacac
     8221 gacgtggtga caatgaacag ccgggttaaa ttccgcaatc ttagcgatgg cgaagtgcgt
     8281 gtgcgcacgc tggtgtatcc ggcaaaaatg accgatagca atactcagct ttccgttatg
     8341 gctccggtag gtgccgcact gctggggctg cgcgttggcg attccattca ctgggaactt
     8401 ccgggcggcg ttgcaaccca ccttgaagtg ctggaactcg aataccagcc agaagctgct
     8461 ggcgactacc tgctttaatc ccatctgcac aagcgataac tcgctttgcc cagaggatgc
     8521 actcgcatcc tctttacgca aaatcagcaa tttttcctgg ttctcactgt tcacttttgc
     8581 tgcaaaatta ttccgtttcg tttgtgggaa taaccatggc agaagcattt tacatcctga
     8641 taggatttct gattatggcg gcgattatcg tcatggctgt cctttacctc gaaaaccact
     8701 cctgacgatc atattgttcc ccccggcatc gcattcacca gacctgaaac cgccttctgc
     8761 gccgcctctg cgctttgtgc accgggtacc agaatcacct tacggcactc ctcttcacgt
     8821 ttttcgaaaa tctcatatcc ccgggcggcc tcttcaaacg gcatatagtg ggtaacaatt
     8881 tcttctggtt tcagtaatcc tttctcaatt aacggtaata attctcccag ccatgcgtga
     8941 acgtgggtct gtcccatttt aaacgtcaac cctttatcaa aggcgtcgcc aaacaggaaa
     9001 ccgtgaataa a
//
LOCUS       DAZGLJ010000178         8941 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000178 DAZGLJ010000000
VERSION     DAZGLJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.178"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            108..536
                     /gene="uspC"
                     /locus_tag="ACHK49_003752"
     CDS             108..536
                     /gene="uspC"
                     /locus_tag="ACHK49_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO9264320.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(543..1967)
                     /gene="otsA"
                     /locus_tag="ACHK49_003753"
     CDS             complement(543..1967)
                     /gene="otsA"
                     /locus_tag="ACHK49_003753"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO9264321.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(1942..2742)
                     /gene="otsB"
                     /locus_tag="ACHK49_003754"
     CDS             complement(1942..2742)
                     /gene="otsB"
                     /locus_tag="ACHK49_003754"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO9264322.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(2909..3895)
                     /gene="araH"
                     /locus_tag="ACHK49_003755"
     CDS             complement(2909..3895)
                     /gene="araH"
                     /locus_tag="ACHK49_003755"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO9264323.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(3910..5424)
                     /gene="araG"
                     /locus_tag="ACHK49_003756"
     CDS             complement(3910..5424)
                     /gene="araG"
                     /locus_tag="ACHK49_003756"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO9264324.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(5494..6483)
                     /gene="araF"
                     /locus_tag="ACHK49_003757"
     CDS             complement(5494..6483)
                     /gene="araF"
                     /locus_tag="ACHK49_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO9264325.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7280..7783
                     /gene="ftnB"
                     /locus_tag="ACHK49_003758"
     CDS             7280..7783
                     /gene="ftnB"
                     /locus_tag="ACHK49_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO9264326.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(7862..8113)
                     /gene="yecJ"
                     /locus_tag="ACHK49_003759"
     CDS             complement(7862..8113)
                     /gene="yecJ"
                     /locus_tag="ACHK49_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO9264327.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMQAIQKAIALIELKFTPQGESH"
     gene            complement(8228..8314)
                     /gene="azuC"
                     /locus_tag="ACHK49_003760"
     CDS             complement(8228..8314)
                     /gene="azuC"
                     /locus_tag="ACHK49_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO9264328.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            8577..8900
                     /gene="yecR"
                     /locus_tag="ACHK49_003761"
     CDS             8577..8900
                     /gene="yecR"
                     /locus_tag="ACHK49_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO9264329.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT         2307 a         2266 c         2047 g         2321 t
ORIGIN      
        1 ataatcagac taatatttac ctgtttgacc gagttgggat tgcgtcgttt ctccattagg
       61 agtaaagctt taatgtcacc tgaagttcac agaataagga acaggttatg agctatagca
      121 atattcttgt cgctgttgcg gttacaccgg aaagtcagca actgctggca aaagcggtat
      181 ctatcgccag gccagtaaag ggacacatca gtttaattac tctcgcttcc gacccggaaa
      241 tgtacaatca attagccgcg ccgatgcttg aagatttacg cagcgtgatg caagaagaaa
      301 cgcaaagctt tcttgataag ttaattcagg atgcggggta tcccgttgac aagacgttta
      361 ttgcctacgg tgagttaagc gaacatattc tggaagtatg tcacaaacat catttcgatt
      421 tggtgatttg cggtaatcac aatcacagtt tcttttcgcg agcatcctgc tcggcgaaaa
      481 gagttattgc ctcaagtgag gtcgatgtgc tgttagttcc acttacggga gattaaccgc
      541 tcctacgcaa gctttggaaa ggtagcaact ttatcgcgct gctggctttc cgcgcttcgc
      601 ggaactatct gctttaggtc gctaatgaag cactcctgcc agtggttaat atcgtttttc
      661 acgataacgt ccagcatttc tgcatgacgg gaaatacgtt ccgccagcga catagtcaat
      721 gcacgatcca gcgcagctgc aacttcgtca cgatcgtagg ggttaacaat taacgccgac
      781 gttaactcgt ttgccgctcc cgcaaattgc gaaagaacaa gaacgcccgg gttggctggg
      841 tcctgagcag caacatactc ttttgctacc aggttcatcc cgtcacgcag tggcgtcact
      901 aagcccacgt cagagtagcg gaatattttc atcagtaatt tacggtcaaa atgctgattc
      961 aaataataaa gcggcgtcca gcctaattgc ccgtatttac cattaattcg tccagcttca
     1021 ttttcgagct gatgacgaat atcctgatag gcttgcacat caccacgcga cgttggtgca
     1081 atctgggtat aacgaatttt accatgatgc tgcggatatt tttccagcaa cgcttcatag
     1141 gcgagaaaac gctctggcaa acctttggaa taatccagcc gttcgacaga aaagatattt
     1201 tgtacgtttt tcagttccgc tttaagttgc gccagttttg gcggcagtgg cccggcagcc
     1261 tgtttggcta tttctttcgg ttcaatgccg atcgggtaga cttctgttcg aaatgctttg
     1321 ccccaggctg tatggctttt tgcgctacgt gtcgtgacgc gggtcaggtt agaaagacaa
     1381 tccaggaacg ccagacgatc gttttctgtc tggaaaccca gcaaatcata atcacaaagc
     1441 tgttcaagca aggtgtcata tgtcggcagc gcgttgaaga tttccggtgt cgggaaagga
     1501 atatgcagaa agaaaccaat gcgattattc actccccgtt tgcgtaattc atgcgcaaat
     1561 ggcaacaggt gataatcgtg gatccagata atgtcatcgt cttgcaacag cggcagtaat
     1621 ttatctgcca gcaacgcatt tacgcgtaga tagccgtccc aggcaggacg ctgaaattgc
     1681 accagatcga gccgataatg aaaagcgggc cagagaacgg cattggagaa ttggttgtag
     1741 tattcgtcaa ggtcctgttc gctgaggtta aaagaggccc acgtaatgtt accttttttc
     1801 acctttttta gcggctgatc ctcattccct gtttcaccac tccagccaaa ccacagtccg
     1861 cctgcggctt tcagtgcccc cagtatgcca acggcaaggc caccggcact ggcggcgtgc
     1921 tcgtctggtg gtgcaatccg gttagatact acgactaaac gactcatagt catcactcct
     1981 gttattttct cttttttgtt gtaatgcggt ggttatcatt tcaagccagc tccagacatc
     2041 cggcacaccc gccagtcgcc atgatgcctg agttgcacct gtgccaattt ttactgacat
     2101 tccgcccagt cggttaacga ctgcgaagcc agattcatcg gttaaatcat cgcccagaaa
     2161 tacgggcgtt cgcccgataa agggagcttc ctgcataaaa gctgcaattg cctcaccttt
     2221 actggtacct ctcggtttga tctcgacaac acactttccc tgctgtaacg ccatttgtgg
     2281 ccagatctga gtaatacgtt gcgctaatgt cattaatgcg tcttcatgct gcggagcctg
     2341 acgataatgc agcgcaaaag ccatcccttt cgcctccagc tccgcgccgg gatactgagc
     2401 gatgactgta tgcagttgca cgctaatatc acgcgcaatc gcatccggca gatgaacgat
     2461 atgtgtttta ccattgatgt cacggcgctc cgccccatgc acgcccgcta acgggaagcg
     2521 ataaggtttt gccagtgcgt caagctccac cattgagcgc cctgatatca atgccaatgc
     2581 accatcactt gcggttgcca gtagctgtag tccttgcaga atattgtcag gcacgacgac
     2641 ctgatcggga tgcggtttga tttccgccag cgttccatca agatcaaaaa accaggcata
     2701 tttcgcggat agttcagggg tttcggttaa cggttctgtc acccggctct cctccttctc
     2761 ttttcttatt gttttgcgca gaacgcgctt tacagacatg taagtataga cagtgtgacg
     2821 ggggtcgcca tttgcaatga agaacagcca accgttgtgg cggctggctg tggtgggaaa
     2881 aaacgttaaa ttgttgtgga aaaaagcatc agacagtgcg tttcgctttt tgcttgtaac
     2941 ggtcgaagat caccgctgcc agcaggatta agccgcgaac cacgtactgc gcgaaaggag
     3001 aaatattaag caggttcatg gcgttttcca cggtgcctaa aattaagata cccgccacca
     3061 catatgagat ttttccgatg ccacctttca gagaaacgcc acctaaaacg caggcggaga
     3121 taacaataag ctcataacca atcgacgtca ttggctgccc actagtcata cgtgaagcca
     3181 gaataattcc ggctatcgca gataccaggc cagagagaac aaagataata attttggtgc
     3241 gaacaaccgg tacacccgcc agacgcgcag cctcttcgtt cccgccaatc gccagggtgt
     3301 tacgaccaaa ggtggtttta ttcagcaaca aaccaaagat aatcagacac gcgacggtga
     3361 gccagattgg cgcaggcaga ccgaaccagt tagcgtaacc aagggcaaag aagctttcat
     3421 cttcgatacc gaccgcttta ccgtctgaaa tgatgtacgc cagacctcga acaatctgca
     3481 tcgttgccag tgttgtgatc agagcattta ttttcagttt ggcgataaca aagccattga
     3541 ccaggccaca gagaatgcca agcagcaacc ccgctgccac gccaatccac aggctttcag
     3601 tcaggttgat aaccaccgcc gtggtgacac ccgcacaggc aattacggag gcgacagaaa
     3661 ggtcaaagtc accggaagca aggcagaaca acatgccaca cgccaccatc cccgacatgg
     3721 aaattgccag gcccaaccct ttcatattaa tgaaggtggc aaaatttggg acaaaaatgg
     3781 cacaggcgat aaagagcacc gcaaacacca ccagcatgcc gtactgatcc cagatacgcc
     3841 cgaagctgaa tgacgactta ggtgcgccag accccgatgt agaaacagaa gacatcatac
     3901 tctccttact caggcaactg cctggctgac tttaggcatc gcaaggctca gtgcctgacg
     3961 ctcatctgcc tgctcgtgta acaattcacc ggcgatttca ccttcccgca tcaccacaat
     4021 ccggtcggca acgccgagga cttcaggtaa gtcgctggag gcaaacagca ccgccacacc
     4081 ctgcgccgcc agcgcataaa tcacgttgta aatttcatgc ttagcaccaa catcaatgcc
     4141 gcgcgtaggt tcatccagca aaatgacctt catctcttcc gataaccagc ggcccagaat
     4201 ggctttttgc tgatttccgc ctgagagatt catgatcagt tgctcagcgc ccggcgtttt
     4261 gatgttgagc gaacgaatgt ggtgatcggc attgttttct tcccaaccgt tgttgattac
     4321 acaaccgccg agcacatgtt tacgtctggc actgatgttg atattgtcgc gaacggagtg
     4381 cacgggaata atgccttccg ctttgcgatc ttccgggcag agcatcatgc ctgcggcaat
     4441 ggcgtggctc ggtttacgaa tatcgatcgg ctgttggtcg atataaacct gaccggcggt
     4501 gatttgcgtc ccgccaaaca agcctttcat taattcgcta cgccccgccc ctaccagacc
     4561 aaacagccca acaatttcac cactgcgaac cgccagactt attggcgtac gcacgcctgg
     4621 tgctttcaca gcatcaagac gcaggcgctc ctcgccataa ctacgaggtt gccagccgta
     4681 gatatcgcca atgtcgcgcc cgaccatcgc ctgcaccagc gcgtcgtggt caacctgctg
     4741 catatcggta aaggttttga cataacgtcc atctttaaag acagtgatgg catcgctgag
     4801 ggcaaatatt tcttccatac ggtgagaaac gtataagatc acccgcccct cttttcgcag
     4861 ttcacgaata acgcggaaaa gattgtcgat ttcgcgggca gaaagggagc tggttggctc
     4921 atcaaaggcg ataattttag cgttacgcgc cagcgccttg gcgatttcaa ccatctgcca
     4981 ctgaccaatg gagagatatt tcagcggcgt gtccgggtca atatccatac caagatgttt
     5041 aagttgtaaa cccgcctcat aattcagcaa tgagcgattc acaatgccgc ctttatgcgg
     5101 cagctggccg agatagatgt tttccgcgac ggtcatttcc ggcacgagat gtagttcctg
     5161 gtaaataatc gccacacccg cgttaagtgc tgcggtcgtg tcggaaaagg acatttcctg
     5221 cccattaatc actacagaac ccgtggttgg cgcatagttg ccgctgagga tttttaagag
     5281 agttgatttt cctgcgccat tttcacccat caacgcatga acctgaccgg catagcagtc
     5341 aaaactaata tccgtcagcg ccttaacgcc gggaaacgtt ttaccgatgc cgcgaaatga
     5401 gagatacggg gtagactgtt gcataacgtc tccgtgaatc actggtcgtt cacaccccct
     5461 ctgcatcatg cagaggggaa tttttccggc aaattactta ccgcctaaac cttttttctc
     5521 cagttcttct ttaaagttgt cacgcgtgat cagtaccacg tcggtaactt cggtaaattt
     5581 tggcggttca acgtcttttg ctacccagtt gtaaagcatt tcgctggatt tatagccatg
     5641 tacgtccggg cttggcagca gggaaccgta gaagccggtt gcctgtgctt tagacagttc
     5701 gctcaccgca tccacaccgt taatgccaat gccgatgata tcggccgctt taaagccctg
     5761 accttccgtc gcgcgtacgc cgcccagcac ggtgctgtcg ttcataccga cgatcagcca
     5821 atgtttaact tccggatgtt gaaccagcat tgagttggca gcgtcaaatg cccccgggat
     5881 gtcgttagat ttggtaggta cctgataaat ttgtttttcc gggaatccgg ccgctttcag
     5941 cgcatccata gatcccgtag tacggcggcg ggcggtatcc agttcgttgg cggtaatcgc
     6001 catcaccgcg ctttctttga catcccagcc acgtttctgc atctctttat acagttcctg
     6061 gccctgacgt tcgccaattt tagtcgccgc catcatcacc agcggaacgg tatccattgg
     6121 cttacctttg gcgttaacaa actggtcatc cacggcaatg actttcatat cgtagccacg
     6181 cgctttcgcg acgatggcag agccgagttt ggggtccgga gtacaaataa cgaaaccttt
     6241 tgcgccactg gcagccaggc tgtcgatcgc gttcaatgtt ttttcgccat ccggcacggc
     6301 aatcttaata acctcaaacc ctaaatcctt cccggcttta tcggcaaact tccattcggt
     6361 ctggaaccac ggctcttccg gttgcttcac cagaaaaccg agcttcaggt tctccgccat
     6421 agcggattgt gacataacgg ctgccagacc aatggctgcc agggctttag taaatttgtg
     6481 catggttctc tccagcttta gtgtcgtttt gtgtagggca aaaacgaatg acattcgtta
     6541 aattaatcgg aaaacaaagc attacctttt aactaaaaga taagtgactg tgttgacata
     6601 gttttagcga gaaattaatt ctccatagga gagcaatatc acatcgcaga attacagtga
     6661 gaacgtgcat aaatttagcg ggaaaagaca taagggaaag ccaatttgtc agacaaattg
     6721 tcgaatgcac agcagattaa tccataagat tagcctggaa atccttgttg tctttggtac
     6781 ccatgcggga tgtcttcttt ttaaccagtc aataggccgc attacctggc gttgagtttt
     6841 tgaaatggtg taataaccgc aactcaaaga tgtggaaaat gcacgtcatt catttcgcca
     6901 ttaattatca ctgtactcat taattaacag aacacgtata atgagagcca tctcgcaaaa
     6961 atgaaaaaac gttttataaa atcatcactt catcatgaat tcaaattcat tgattaatat
     7021 caacaagata caaaaagcgc tatcattaaa attcattgca gttacattga tttcatcaat
     7081 gaaatgtaaa aatatataaa cttgatgatt taagcatttt cttatacccg ttcagacgtt
     7141 attcttattt cagatcatcg tcagaattga ctccacgatc acatttcgga ccggcagaaa
     7201 ggaattattc tgcaaacagt aattatggtg ttttgattta tcttgcacct ctccacttct
     7261 ggatataagg atattaggta tggcaaccgc tggaatgctt ctcaaactca actctcaaat
     7321 gaaccgcgag ttttacgcat ccaatctcta ccttcacctg agtaactggt gttctgaaca
     7381 gagtctgaac ggcaccgcca ctttccttcg cgcccaggca cagagtaatg tgacccaaat
     7441 gatgcgcatg tttaacttta tgaagagtgt cggcgctacc cccatcgtta aagccattga
     7501 tgttcccggt gaaaaactga actctctgga agaactgttc caaaaaacga tggaagaata
     7561 cgagcaacgt tctagtacgt tggcacagtt agccgatgaa gcgaaagaac tgaatgatga
     7621 ttcaaccgtc aatttcctgc gcgatctgga aaaagaacag cagcatgatg gtctgttgct
     7681 gcaaaccatt cttgatgaag tgcgcagtgc gaaacttgcg ggtatgtgcc ctgtgcagac
     7741 cgaccaacat gttctgaatg tcgtgtcaca ccagctgcat tgatcatcat cggcgctaat
     7801 gcattgcgcc gatgaaggtt ttgagaaacc gcagcctcat ctgtttgaag cagcggtttt
     7861 tttaatggga ttcaccctgt ggggtaaact tgagttcaat aagcgcgatg gctttttgga
     7921 ttgcctgcat ggtgaccggg tctgcggcgg cgggatggtt agtaaagtcg atattcttca
     7981 gctgactgga cattttttca cgaacttcaa cgggcgcgat tacatcgaga acatccagaa
     8041 tttgtttgat aaccaactgg caagcaacca catcagaaac cagttcctga tcggcattca
     8101 gcggctggga catcgtaaac tcctgatagc attttgaaag ccgttatagt agcggcttca
     8161 catcttcagc gatagtcaca tccaccgtca tcaggacaca aaaaaacctg ccggagcagg
     8221 ttttttatta tcggaacata ttgcctggcg gtacgtcttt gaacgtcttg caatagttat
     8281 tgaacatact tttcaggatt ttgcgcagtt tcatcgcggc actccgacca tttgttatac
     8341 aggtgttatt ctctttgcgc ttataatatg acaaccatca caaaaatcaa tctttatgtg
     8401 atacaaatca cataaatacc cctttaatgt tataaaaatg ataatcaaaa aacagccccc
     8461 ctatttctga cacctacaga tggcaagaaa tagcgcctgc caggcgtctt ttccggccat
     8521 tgtcgcagcg ctgtaacgcg taaaatagtg ctttctctta ctcttctggc tggaccatga
     8581 gacttctgat tctgactctt tcattaataa cgctggcggg gtgtacggtt actcgccagg
     8641 cccatgtgag cgaagttgac gcggcaacag gtattgtacg gttggtttat gaccaggctt
     8701 ttttgcagca tgcccatacc gatcgttatg tcagtcgcgg gatcgccgat cgcgcatgcc
     8761 agcaggaagg ctatacccac gcggtcccct ttggtcagcc agtaggcaac tgtagccttt
     8821 ttgcgggttc cttatgtttg aatacggaat tcactttgtc atatcagtgc catcattccg
     8881 ccttccctgt ctttctctaa atattttaat cagcgagggg atcttcgctg attaaagaaa
     8941 t
//
LOCUS       DAZGLJ010000179         8915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000179 DAZGLJ010000000
VERSION     DAZGLJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.179"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1036
                     /locus_tag="ACHK49_003762"
     CDS             <1..1036
                     /locus_tag="ACHK49_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO9264330.1"
                     /translation="GSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNK
                     KKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLW
                     AFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKL
                     FVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDI
                     NQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKI
                     IAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKSE
                     GGNKVIIHHI"
     gene            1217..2707
                     /gene="dgcJ"
                     /locus_tag="ACHK49_003763"
     CDS             1217..2707
                     /gene="dgcJ"
                     /locus_tag="ACHK49_003763"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO9264331.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2750..3253
                     /gene="yeaK"
                     /locus_tag="ACHK49_003764"
     CDS             2750..3253
                     /gene="yeaK"
                     /locus_tag="ACHK49_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO9264332.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3254..3358)
                     /gene="yoaI"
                     /locus_tag="ACHK49_003765"
     CDS             complement(3254..3358)
                     /gene="yoaI"
                     /locus_tag="ACHK49_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO9264333.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3528..3974
                     /gene="yeaL"
                     /locus_tag="ACHK49_003766"
     CDS             3528..3974
                     /gene="yeaL"
                     /locus_tag="ACHK49_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO9264334.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(3931..4752)
                     /gene="nimR"
                     /locus_tag="ACHK49_003767"
     CDS             complement(3931..4752)
                     /gene="nimR"
                     /locus_tag="ACHK49_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO9264335.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            4849..6030
                     /gene="nimT"
                     /locus_tag="ACHK49_003768"
     CDS             4849..6030
                     /gene="nimT"
                     /locus_tag="ACHK49_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO9264336.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRTLHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6085..6432
                     /gene="yeaO"
                     /locus_tag="ACHK49_003769"
     CDS             6085..6432
                     /gene="yeaO"
                     /locus_tag="ACHK49_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO9264337.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6454..6708)
                     /gene="yoaF"
                     /locus_tag="ACHK49_003770"
     CDS             complement(6454..6708)
                     /gene="yoaF"
                     /locus_tag="ACHK49_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO9264338.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            6891..7916
                     /gene="dgcP"
                     /locus_tag="ACHK49_003771"
     CDS             6891..7916
                     /gene="dgcP"
                     /locus_tag="ACHK49_003771"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO9264339.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            7949..8047
                     /gene="yoaK"
                     /locus_tag="ACHK49_003772"
     CDS             7949..8047
                     /gene="yoaK"
                     /locus_tag="ACHK49_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO9264340.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8050..8124
                     /gene="yoaJ"
                     /locus_tag="ACHK49_003773"
     CDS             8050..8124
                     /gene="yoaJ"
                     /locus_tag="ACHK49_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO9264341.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8183..8431)
                     /gene="yeaQ"
                     /locus_tag="ACHK49_003774"
     CDS             complement(8183..8431)
                     /gene="yeaQ"
                     /locus_tag="ACHK49_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO9264342.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8659..>8915)
                     /locus_tag="ACHK49_003775"
     CDS             complement(8659..>8915)
                     /locus_tag="ACHK49_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO9264343.1"
                     /translation="GGQVLAVPPAYTSQRCACCGHTAKENRLSQSQFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT         2198 a         2161 c         2107 g         2449 t
ORIGIN      
        1 cggcagtaca ctgatccaga caaagtcgaa tgatgtttct attttctata ttttccgcca
       61 actcagtttt atttgtttaa cctcgctggc actcttttgt tatggaaaag acaacatcct
      121 tgacaacaat aagaaaaaaa cgggaatcct gttgctggcg ctgattcctt ttttagtttt
      181 tccccttctg gcacacaatc tgagcagtta taacgctgac tattctttgt atgtcgtcga
      241 ctactgtccg gacaaccata ctgcgacctg gggaatcaac tatacaaaaa tattggtttg
      301 tctgtgggca tttttactgt tctttattat catgcgtaca cgattagcca gcgaactatg
      361 gccgttaata gcattattat gtctggcatc gctatgctgc aacttacttc tactgactct
      421 ggatgagtat aattacacca tctggtatat cagtcgcggg attgaagttt ccagtaaact
      481 gtttgttgtg tcttttctga tttataacat ttttcaggaa ctgcaactct ccagcaaact
      541 ggcagttcat gatgtgctga ccaatattta taatcggcgc tactttttca acagcgtaga
      601 gtcattattg tcgcgacctg ttgttaagga cttctgtgtc atgctggttg atattaatca
      661 gttcaaacgc atcaatgccc aatggggaca tcgtgtgggt gataaagtgc tggtttcaat
      721 tgtcgatatt atccagcaaa gcatccgccc tgatgatatt ttagcgcgac tggagggtga
      781 ggtgtttggc ttgctattta ccgaactcaa tagtgcccag gcaaaaatca ttgcggaacg
      841 tatgcgtaaa aatgtcgaac tcctgaccgg ctttagtaac agatatgatg ttcctgaaca
      901 aatgaccatc agtattggca cggttttttc aacgggtgac acgcgtaata tctcgcttgt
      961 catgacggaa gcagataaag ccttacgcga agcgaaaagc gaggggggca acaaagtgat
     1021 tattcatcat atttaagtgc aaaaatattc agagccatgc tttttgcgtg gcttttgcat
     1081 acaatttatt actattaccc ctaaatttcc cctccccagc ggcgcagaga tgagtataat
     1141 tagcgcccct gtgccaggcc gcaatcgaac tttatctggt tttctcgttt cactaaccga
     1201 aggagtgcca tttatcatga aattgcacca tagaatgctc cggcatttta tcgccgcaag
     1261 tgtcattgtg ctgacatctt ccttccttat ttttgaactt gtcgccagcg acagagcaat
     1321 gagtgcctat ctgcgctata tcgtgcagaa agcagactcc tcctttcttt atgataagta
     1381 tcagaatcag agtattgccg cgcatgtgat gcgcgctctc gctgctgagc agtcggaagt
     1441 gtcgccagaa cagcggcgcg ccatctgcga ggcttttgag tctgccaata atacccatgg
     1501 cttaaacctg actgcccata aatacccggg cttacgcggc acactacaaa ccgcatccac
     1561 tgactgcgac acaattgtgg aagctgcagc actattaccc gcttttgatc aggcagtgga
     1621 aggcaaccgc caccaggatg attacggttc aggtcttggg atggccgaag agaaatttca
     1681 ctattatctc gatctcaatg accgctatgt ctatttttat gagccggtta atgttgaata
     1741 ctttgcgatg aataactggt ccttcctgca gtcaggaagt attggcatcg atcgcaaaga
     1801 tattgaaaag gtatttaccg ggcgtaccgt attgtcgagc atttaccagg atcagcgtac
     1861 taaacagaac gtgatgagtt tgctgacgcc ggtatatgtc gcagggcagc taaaagggat
     1921 tgtgctgctg gatattaaca aaaacaattt gcggaatatc ttttacactc atgaccgccc
     1981 tctcctctgg cgttttctca atgtcacgct aaccgatacc gattcggggc gcgacattat
     2041 catcaaccag agcgaagata atctgttcca gtacgtcagt tacgtccatg acttaccggg
     2101 cggcattcgt gtctcgttat ccattgatat tctttacttt atcacgtctt cgtggaaaag
     2161 cgttctgttc tggattttga cggcgttaat tttgctgaat atggtccgga tgcacttccg
     2221 tttataccaa aatgtgtcgc gagaaaatat tagtgatgcg atgactggac tgtataatcg
     2281 caaaatttta acccctgaac tggagcagcg gttgcagaaa ctggtgcaat ccggttcttc
     2341 ggtgatgttt attgcaattg acatggacaa gttaaagcaa ataaatgaca ccctcggtca
     2401 tcaggagggg gatttagcga ttacgttatt agctcaggcg attaaacaat cgattcgtaa
     2461 aagtgattat gccatccgac tcggtggcga tgaattctgc atcattcttg tcgattcgac
     2521 gccgcaaatt gcagcacaac tgcctgaacg tatcgaaaaa cgtctgcaac atatcgcgcc
     2581 gcagaaagaa atcggcttct cttccggtat ttacgcgatg aaagaaaacg atacgttaca
     2641 tgatgcgtat aaagcttccg atgagcgttt atatgtcaat aagcagaaca aaaacagccg
     2701 ttcatgataa ccttctgtgg ttgtttgctt gtaatctcag gagcgtgaaa tgactgaaat
     2761 ggctaaagga agcgtgacgc atcagcggtt aatcgcttta ttatcacaag aaggtgctaa
     2821 cttccgcgtc gttacccatg aagcggtagg taaatgtgaa gcggtgtcag aaattcgtgg
     2881 caccgctcta ggccagggtg ctaaggcact ggtatgtaaa gtcaaaggca atggcgtaaa
     2941 tcaacatgtt ctggcgatcc tcgctgccga tcaacaggcc gatctgagcc aactcgccag
     3001 ccatataggc ggattacgcg cttcgttggc cagcccggcg gaagtcgatg aactcacagg
     3061 ctgtgtcttc ggcgcgatcc cccctttcag cttccatcca aaactcaaac tggttgccga
     3121 ccctctactc ttcgaacgat ttgatgaaat cgccttcaat gcagggatgc tggataaatc
     3181 cgttattttg aaaaccacgg attatttgcg cattgcgcaa ccagaactgc tcaatttccg
     3241 ccgcactgcg taactagcct gtccgttcaa taagcagaac ggacaaaacc agcacaacag
     3301 caatggcaaa aaacgatgac gtgataatca gtgtctcgac gaacatttga tcgttcatag
     3361 catgcccccg ggagatagct gttttcacgt tattgttagc gtgcacaaat ggcagtttga
     3421 tgacagttcg cctgattttt tatgcaaaaa acgtaaagat tttctacttc cttcctgcag
     3481 caagcgtaaa gtaagcaggc ttattatttt ttggcaagga aaccacgatg tttgatgtca
     3541 ctttgctgat cctgctcggg ttagctgcgc tgggctttat cagtcataac accactgtcg
     3601 ccgtttcaat tctggtgttg atcattgtcc gcgtcacacc gttaagcacc tttttcccgt
     3661 ggattgaaaa acaggggcta agtatcggga taatcattct gacgattggc gttatggccc
     3721 ccatcgccag cgggacgcta ccgccctcaa ccttgattca ctcgtttctg aactggaaat
     3781 cgctggtggc aattgcagta ggcgttattg tctcctggct gggtgggcgc ggcgtgacct
     3841 taatgggcag ccagccgcaa ctggtcgccg ggctgctggt aggcactgtt ttaggcgtgg
     3901 cgctgtttcg cggcgtaccg gtcggcccgc ttattgccgc gggtctggtt tcgctgattg
     3961 tggggaaaca gtagttaatc ccgcaatata tcggcctggc gtttgcccca ggcctttctt
     4021 aaacatagta ataaaggccg ttgttgaatc ataccccagc gtatgagcaa ctttctgtac
     4081 agtatcgcct ttcactaatc cctgaagtgc cataatcagc tgtaactgct gacgccactg
     4141 gcgaaaactc aaccccgtct cttttactat cagtcgcgca agattgcgtt cactcatcgc
     4201 aaaaaagcca gcccattgcc ctaatgaccc ccactcgacg ggccctttcg ccatcatctc
     4261 caccatcgtg cggattttag gatgagaaga aacgggcagg tgcagctgtt gttgtggctg
     4321 ctgagggagt tcatcaaaca ggacctgaat taatctgcgg gtcatcggct cagcacgctg
     4381 agtcgtcgtc ctgtttgcca gggttaaaat caattcccgg cataagggcg atattttcaa
     4441 tgtgcaacat gttgtcggca ttgtcactgc gctgggttcg ataaacagaa aacagagttc
     4501 agcgttagct gtcacttgat tgctatgttc caccccgccc ggtatccaaa ccgcatattg
     4561 cggcggcacc atccacaaag cattttccac cgtacaggta attgcaccat gtaatgctag
     4621 tatcagctgt cctttgcggt gctgatgtac aggactggtt aactcatccg ttccggcgtg
     4681 aatacaaaat gcgaccgcgg cttcatggtg tcggtcgggt tcatagccat tgagattcaa
     4741 cctgtgcatc attttgtccg aatttagcga taatttgtca ttttagcttg attcaacata
     4801 acaataaaaa cggtaaggta cagcctcgtt tgtaacaatg agaagcatat gacctgttca
     4861 acttcattaa gcggcaaaaa caggattgtc cttatcgctg gcattctgat gattgccact
     4921 acattacgcg tcaccttcac cggcgcagca ccgttactcg atacgattcg ttccgcttac
     4981 tcgctgacga cagcgcaaac cggcttatta accaccctgc cattattggc ctttgcgcta
     5041 atctcacctt tggctgcccc ggtggcgcga cgttttggta tggaacgtag cctgtttgcc
     5101 gcgttacttt tgatctgtgc aggtatcgca attcgttctc tcccttcgcc ttacttatta
     5161 tttggcggta cagcggtcat tggcggtggg attgcattag gcaatgtctt actgccagga
     5221 ttaattaaac gcgatttccc tcattccgtc gccagactta ccggcgcata ttccctgaca
     5281 atgggagctg cagcggcact gggatcggct atggtcgtgc cgctggcttt gaacggtttt
     5341 ggctggcaag gcgcgttgtt catgctgatg tgttttcctc tgctggctct ttttttatgg
     5401 ctgccacagt ggcgaagcca acaacatgca aatttgagta cctcgcgcac cttacatact
     5461 cggggtatct ggcgttcgcc gcttgcctgg caggtcacat tgtttcttgg gatcaactca
     5521 ctggtctatt acgtgattat tggctggctt ccggcgatcc tcatcagtcg cggctatagc
     5581 gaagcacagg cgggttcact gcatggtttg ctgcaactag ccacagcagc tcccggtttg
     5641 ctgatccctc ttttcttaca tcatgtgaaa gatcagcgtg gtattgcagc gttcgttgcc
     5701 ttgatgtgcg cagtgggcgc tgttgggctc tgctttatgc cagcgcacgc gatcacctgg
     5761 actctgcttt tcggttttgg ttccggcgca acaatgatac tggggttgac gttcattggt
     5821 cttcgggcaa gttctgcgca tcaggcggcg gcactctcgg ggatggcaca atccgtcggg
     5881 tatttgctgg cagcctgtgg gccgccgctg atgggtaaaa tacacgatgc taacggtgac
     5941 tggtccgtac cactgatggg tgttgccata ctttcactac tgatggcgat tttcggactt
     6001 tgcgccggga gagacaaaga aattcgctaa tatccggtgc tatagtgacg tgacaaatca
     6061 tgcgtgaaag ggagaacaaa cacgatgaat attcagtgca aacgcgttta tgatccggct
     6121 gaacagagcg atggttatcg cgtactggtc gaccgcctct ggccgcgcgg tatcaaaaaa
     6181 accgatttag cccttgatga gtgggataaa gaaatcacgc cgtcaacgga actgcgcaaa
     6241 gcctttcacg gcgaagtcgt cgattttgca acctttcgcg agcaatatct tgcagaactg
     6301 gcgcaacacg agcaagaagg aaagcggctg gcggacatcg ccaaaaaaca gccgctgacc
     6361 ctgctctacg cagcaaaaaa caccacgcag aaccatgcgc tggtgctggc cgactggcta
     6421 cgtagcttgt gattttagta cagcatccgg cggttatttt tcaccagccg gatggtcccg
     6481 tcgccacaat gcccattcat caatcgtttc accgcccggt aatttgcaat tgttgctgac
     6541 cccttgcgct gtctgcactg gaatgagcgt cccgcccttc tgctggcaat agaccgacgc
     6601 cggatttgcc ataccaatct gcggcggttt aggtgcttct ggctgagaag aggttgaaca
     6661 accagccagg accagcaagc aaggcaaagc aaaactgata attttcattt attgatctca
     6721 cacatttatc tacgcttaga gtatcgcggt atcgttttgt tttgcagcac tatttttatt
     6781 acattcactc aaaacatatt acgtcttgtt tcatctttgt tgatgatgtt ttatcatgcc
     6841 tgcaaagatt aaataatcag catttacccg ccgtatcctg gagttgtttc gtgtcagatc
     6901 agattatcgc ccgcgtctcg caatcccttg ccaaagaaca gtcactagaa agtctggtcc
     6961 gacagcttct ggagatgctg gaaatggtca ctgatatgga atcaacctac ctgaccaaag
     7021 tggatgtcga agcgcgcctg cagcatatta tgtttgcccg taacagccag aaaatgcaca
     7081 tcccggagaa ttttaccgtc tcgtgggatt actcgttatg caaacgcgct attgatgaaa
     7141 actgcttttt cagcgatgaa gtccctgacc gttggggcga ctgtattgcg gcacgcaatc
     7201 ttggaatcac cacatttctg agtacaccaa ttcacttacc ggatggatca ttctatggca
     7261 cgctttgcgc cgccagcagt gagaagcgcc agtggagtga acgcgcggaa caggttttac
     7321 agttattcgc cggactgatt gcacaatata ttcaaaaaga ggcgttggtt gaacagctgc
     7381 gcgaagccaa tgccgcgctg attgcgcaat cgtataccga ctcgttaacc gggctaccga
     7441 atcggcgggc gatttttgaa aatctgacga cgctgttttc tctcgcccgg catcttaacc
     7501 ataagataat gatcgcgttt atcgatctgg ataacttcaa attaatcaat gatcgttttg
     7561 gtcataatag tggcgatctg tttctcattc aggttggcga gcgccttaat acgctccagc
     7621 aaaatggcga agttattggt cgtctcggcg gtgatgagtt tttggttgtt tcactaaata
     7681 acgagaatgc ggatatttcg tcgctgcgag aacgtattca acagcaaata cgtggagaat
     7741 atcacttagg tgatgttgat ttgtattatc ctggtgccag tcttggcatt gtagaagtcg
     7801 atcctgaaac gaccgatgca gacagtgccc tgcatgctgc cgatatcgcg atgtatcagg
     7861 agaaaaaaca caaacagaaa acaccttttg tcgcgcattc agcgccacat tcctgaggcg
     7921 tattcacatc cttttgattg gtgataacat gcgaatcggt attatttttc cggttgtaat
     7981 ctttattaca gcggtcgtat ttttagcatg gttttttatt ggcggctatg ctgccccggg
     8041 agcataaaga tgaaaaaaac aacgattatt atgatgggtg tggcgattat tgtcgtactc
     8101 ggcactgagc tgggatggtg gtaacgtcac ctctaaaaaa tagcaaaggc tgcctgtgtg
     8161 cagcctttgt gcaatttaag cgttaacttt taatcttcct gtagataaat agcacgacaa
     8221 tcgcaccaat aacggcaacc acgaagctgc caaaattgaa gccatcgact ttaccaaagc
     8281 caaacagcgt gctgatccat ccgccaacta cggcaccgac tatccccagc aggatagtca
     8341 taaagaatcc accaccatct ttacctggca tgatccactt cgccagaata ccggcaataa
     8401 gcccaaaaat aatccatgac agaatgccca ttgtttcctc acttatctgt tttgcattag
     8461 cgggttagtc gctgataaaa agcatagcac aacatcggga gggcaagatt tgtgacgagc
     8521 atcacggagg atttttttgc ggatggcaca gaaattgcgc catcaacgat cagtgataat
     8581 taccaaccac aaacatcatg ttcgttttcc gtgtcttgac atcctccacg ccctgaatga
     8641 cgaggatccc tgctacgttc aggctgtcgc ctgaatcatt tcggtgggtt cctgcttcaa
     8701 cgggcggcct gactgcacca tctctccaca ggcaagaacg gcgtgccccg ccgctaaaat
     8761 gttacgagcg ccgtttacat cggcgttcgc tgtatatcca catacctggc atctgaattg
     8821 actttgtgac aggcgatttt ctttcgctgt atgaccacag cacgcgcaac gctggcttgt
     8881 gtacgctggc ggaacagcaa gcacctgacc gccac
//
LOCUS       DAZGLJ010000180         8854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000180 DAZGLJ010000000
VERSION     DAZGLJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.180"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(77..3439)
                     /gene="mscK"
                     /locus_tag="ACHK49_003776"
     CDS             complement(77..3439)
                     /gene="mscK"
                     /locus_tag="ACHK49_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO9264344.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(3567..4214)
                     /gene="acrR"
                     /locus_tag="ACHK49_003777"
     CDS             complement(3567..4214)
                     /gene="acrR"
                     /locus_tag="ACHK49_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO9264345.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQVKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            4356..5549
                     /gene="acrA"
                     /locus_tag="ACHK49_003778"
     CDS             4356..5549
                     /gene="acrA"
                     /locus_tag="ACHK49_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO9264346.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            5572..8721
                     /gene="acrB"
                     /locus_tag="ACHK49_003779"
     CDS             5572..8721
                     /gene="acrB"
                     /locus_tag="ACHK49_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO9264347.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDREKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
BASE COUNT         2133 a         2270 c         2312 g         2139 t
ORIGIN      
        1 tcacgccgca tccggcatga acaacgcgca ctttgtcagt aatctcgggg caactaaatg
       61 tcgccccctt tggttattac cctaccgctg gcgtcgggtc atcgcctttg tagtcgcgtt
      121 tgacttccgt cacctcatcg cccttctcgt tgtgcagatg cacttcaagc tggttaaagg
      181 caatgttgat gtcgttttca cggcacagct gatcgatagt acggttcagc tcatcgacag
      241 tacgactacg gtcacgcagt tcacgcacat acagacgcag ctcatgatcc aacgtgctgg
      301 caccaaacgc cgtaaagaag acttccggca ttggttcgtg catcaccctt gggtgctcag
      361 tcgccgcctt cagtaacact ttacgcactt tttccagatc ggagccatag gccacgccga
      421 gacggatcac cagacgcgta gtagtgtcag tcaacgacca gttgatcaga cgctcggtaa
      481 caaacgcttt gttcgggatg atcacttctt tgcgatcgaa atcggtaatc gttgtcgcac
      541 gaatacggat cttacttacc gtccccgaga agctaccaat ggttaccgta tcgccaatac
      601 gcaccggacg ttcgaacaga atgatcaaac cggagacgaa gttaccgaaa atttcttgta
      661 aaccaaaacc aagacctacg gataatgctg cggccagcca ctggagttta tcccaagaga
      721 cgcccagcga tccgaacacc gtcatcgcac caacggcaat aatgatgtag ttaaggatgg
      781 tagtaatggc atacgacgcg ccctgacgca tattcagtcg cgagagcacc agcacttcca
      841 gtaaaccagg caggttgcga atcaacgccc aggccaccat tgaggcgata atcgcaaaca
      901 acagactgcc catggtgacg tttttcacca ccgcagcgcc agcttcagtg ccgttgtaat
      961 gccagagcgt gatgctgtcg agatagctga acacggtgat caaatcggac caaattgccc
     1021 agaacatgac accgaacagc gcaaacatca gcaacatggt aatacgcagc gtctgctggt
     1081 taacctgttc cagtgcaatg gtgggttctt ccggcggttc ggcaccttct gcgccctctt
     1141 tcaccagatt ctgccgacgc gccaacgcac gacgccaggc gatacgccgc gccgctacgc
     1201 ttaagccgcg caataccgtc tggtagagca agttccagat gatcaccaga taaacggttt
     1261 caatccaacg cccggagagg cgcagcgtgg tgtagaaata acccgtggca gtcagcacca
     1321 tcaatgcaat cgggataatc gacagcacgg taatggtgac cagtcgcatg gtgtgcgact
     1381 ctttatcacg ccagctttcg cggcacattg gccataccag gaaggcaatc agcagcaggt
     1441 tgaagaaaat catcgcctgc cccagcacat catccatcag atgcagcggg gaaagttctg
     1501 ccaccacaga ccagaaatgg ataggcagca acgcgagact gatgcggaca atttgccgac
     1561 gccagtggct ggtctgctgc tccggcatgc cgaagtgacg cacggcaacg ccatttttct
     1621 ccagtacctt ccagcacagg ccaaacacca gccagaaaat cgccagtttt ttactgaacg
     1681 accatagcag ttcgctgatg ttgagctgca tggtcaacag aatcaggcca accgcgagaa
     1741 taatcaggca caccggcagc gcgcggatca ggtcgataag gatcgctttt ggtgtgttga
     1801 gctggctatc gttacgcagg gaacccacag cggaagccag tttctgttga tacgctttca
     1861 gccagcccag acgccagtgg atcagcccgg caatcaacag cagcggcaaa ccggcgagga
     1921 aagcgataaa aacggcaggc caggcttttt cccagttcac cgtgattttc atcgacttaa
     1981 attcatcttt caggctttgc gggaacgctt tgatccagtc ccagtccatt ggacggttac
     2041 tgttcaccca aaagatttgc tgagtcagga tggatttcag gtttttcgat acgctcatta
     2101 actgctgctg gttgatttgc aggttaatgg ccatcatcag ctggttaccc aactgtttgt
     2161 tgagttgatc cagcaattcg cgacgcatat caaccacttg caataacgca tcgtgaactt
     2221 cgctgttgac ttcgttggtg tgaccttctt ccagtttgtt gacgaacgca tcgctctgga
     2281 agagtgcatc acgctgctgg ttaacttcaa actgttcgag acgcaaatcc gcgatgcggt
     2341 tggtcatgtt ttccagttca tccgccgagg gcagcgtttg ttgttgctgg taaaggatac
     2401 gagacaacag caggctgccc ttcaggacgg caatctgctc tttaatattg cgttccgatt
     2461 gcagcgcccg ctccagccag tttttgactt taatgttttg ctgcatcaac tgattaccgt
     2521 tttcagtcgc ggtaatcaga cgctgactta actgctggtt aatttccagt tcctgtttca
     2581 ccagcggatt agcctgaata cgcgcggctt catccgggga gacggcttcc tgcgccgttt
     2641 tttcagttaa agtcaggcgc ttgctgttta ccgcttcttg caacagctgc aactggtgct
     2701 ccagacgggc gctgttcgcc gtcacgtaat cacgttgctt ttgcaaggta tcctgcaaga
     2761 cggtgttccc ttccaggctt ttacgctgct ggtcaatctc cgcattcagc aacgcctgct
     2821 gagcctgcat taacactttc tggctgggac gtaaggcagt ctcgccgaca tcagtcccat
     2881 ccagacgact gcgaatttgt tgcagctgct gcgaagcgtt atacatcgca ttttgcacgc
     2941 gttcgggctg cgtctgtaac gaaaccagct ggctgttata agacgccaga tcgttttgtg
     3001 cgttttgcaa atcgtccagc gcctgggcaa cgcgagtttc cagctggcgc aacgaaagcg
     3061 tactcagaat tttgcgcgtt tcttcgtcgt tatcgacatc gctaagtgct gttaacgccg
     3121 cggtcgcctg gcgcattttt tccggcgctt cagcgacttt ttgtcgtagc tgaactgtct
     3181 cttcttttat gcgatcgatt ttatcgaggg tggctaatgt atctgtcaga tcctgctgca
     3241 ccagtttgtc ctgagcagaa agatcttttt gtttatttag tgagtcaagt tgcgcctgca
     3301 ggtccgcttt tgtcggcaga tcaccattcg atgacgcccg cgcaaacgcc gtgttctggc
     3361 ataacaagac aaaaacaaaa gcaataaatg ctgaaaaaac aaaatgccgt gatcgtttgt
     3421 aatactggaa catagtcatg atgaatgaag gtttctgaac ctgaagaacg acctgaaaaa
     3481 gtcaaaccgc aagaatatca cgacgcagtg aaccagaata gcaacgacga aaatgtccag
     3541 gaaaaatcct ggagtcagat tcagggttat tcgttagtgg caggattacg aagcgtgggg
     3601 cacaggagat acatctccag taagatggca acgtaatcgc gggcttcttt tttaagatca
     3661 aaagattgcg gggcaaagag ccagttttcc atcaggccgg aaatatagcc gcgcataata
     3721 attgctgcgc gacgcgtcat taaatccgca ggcaacattt tcgcttcaat acaatgtttt
     3781 aacgtttgtt ctatacggtc ataactttcc agacagagat tacgttgtgc ctgttgcaca
     3841 acagccattt ctccgacaaa ttcgcatttg tggaatataa tctccatcaa taatcgacgc
     3901 cgttcttctg tcaccgtgga ttcaagaaca tgaattaata tctctcttaa tactgagagt
     3961 ggatcgccag ggaattttac ctgatactca agctctagtt caccaatatt ggattctgac
     4021 agttcccaga tctcactgaa caaatccgac ttgtctttaa aatgccagta gattgcaccg
     4081 cgcgtaacgc cagctgcttt tgcaatctca cccagcgagg tggatgatac cccctgctgt
     4141 gagaaaagac gtagagccac atcgaggatg tgttggcgcg tttcttgcgc ttcttgtttg
     4201 gtttttcgtg ccatatgttc gtgaatttac aggcgttaga tttacataca tttgtgaatg
     4261 tatgtaccat agcacgacga taatataaac gcagcaatgg gtttattaac ttttgaccat
     4321 tgaccaattt gaaatcggac actcgaggtt tacatatgaa caaaaacaga gggtttacgc
     4381 ctctggcggt cgttctgatg ctctcaggca gcttagccct aacaggatgt gacgacaaac
     4441 aggcccaaca aggtggccag cagatgcccg ccgttggcgt agtaacagtc aaaactgaac
     4501 ctctgcagat cacaaccgag cttccgggtc gcaccagtgc ctaccggatc gcagaagttc
     4561 gtcctcaagt tagcgggatt atcctgaagc gtaatttcaa agaaggtagc gacatcgaag
     4621 ctggtgtctc tctctatcag attgatcctg cgacctatca ggcgacatac gacagtgcga
     4681 aaggtgatct ggcgaaagcc caggctgcag ccaatatcgc gcaattgacg gtgaatcgtt
     4741 atcagaaact gctcggtact cagtacatca gtaagcaaga gtacgatcag gctctggctg
     4801 atgcgcaaca ggcgaatgct gcggtaactg cggcgaaagc tgccgttgaa actgcgcgga
     4861 tcaatctggc ttacaccaaa gtcacctctc cgattagcgg tcgcattggt aagtcgaacg
     4921 tgacggaagg cgcattggta cagaacggtc aggcgactgc gctggcaacc gtgcagcaac
     4981 ttgatccgat ctacgttgat gtgacccagt ccagcaacga cttcctgcgc ctgaaacagg
     5041 aactggcgaa tggcacgctg aaacaagaga atggcaaagc caaagtgtcg ctgatcacca
     5101 gtgacggcat taagttcccg caggacggta cgctggaatt ctctgacgtt accgttgatc
     5161 agaccactgg gtctatcacc ctacgcgcta tcttcccgaa cccggatcac actctgctgc
     5221 cgggtatgtt cgtgcgcgca cgtctggaag aagggcttaa tccaaacgct attttagtcc
     5281 cgcaacaggg cgtaacccgt acgccgcgtg gtgatgccac cgtactggta gttggcgcgg
     5341 atgacaaagt ggaaacccgt ccgatcgttg caagccaggc tattggcgat aagtggctgg
     5401 tgacagaagg tctgaaagca ggcgatcgcg tagtaataag tgggctgcag aaagtgcgtc
     5461 ctggtgtcca ggtaaaagca caagaagtta ccgctgataa taaccagcaa gccgcaagcg
     5521 gtgctcagcc tgaacagtcc aagtcttaac ttaaacagga gccgttaaga catgcctaat
     5581 ttctttatcg atcgcccgat ttttgcgtgg gtgatcgcca ttatcatcat gttggcaggg
     5641 gggctggcga tcctcaaact gccggtggcg caatatccta cgattgcacc gccggcagta
     5701 acgatctccg cctcctaccc cggcgctgat gcgaaaacag tgcaggacac ggtgacacag
     5761 gttatcgaac agaatatgaa cggtatcgat aacctgatgt acatgtcctc taacagtgac
     5821 tccacgggta ccgtgcagat caccctgacc tttgagtctg gtactgatgc ggatatcgcg
     5881 caggttcagg tgcagaacaa actgcagctg gcgatgccgt tgctgccgca agaagttcag
     5941 cagcaagggg tgagcgttga gaaatcatcc agcagcttcc tgatggttgt cggcgttatc
     6001 aacaccgatg gcaccatgac gcaggaggat atctctgact acgtagcggc gaatatgaaa
     6061 gatgccatca gccgtacgtc gggcgtgggt gatgttcagt tgttcggttc acagtacgcg
     6121 atgcgtatct ggatgaaccc gaatgagctg aacaaattcc agctaacgcc ggttgatgtc
     6181 attaccgcca tcaaagcgca gaacgcccag gttgcggcgg gtcagctcgg tggtacgccg
     6241 ccggtgaaag gccaacagct taacgcctct attattgctc agacgcgtct gacctctact
     6301 gaagagttcg gcaaaatcct gctgaaagtg aatcaggatg gttcccgcgt actgctgcgt
     6361 gatgtggcga agattgagct gggtggtgag aactacgaca tcatcgcaga gtttaacggc
     6421 caaccggctt ccggtctggg gatcaagctg gcgaccggtg caaacgcgct ggataccgct
     6481 gcggcaatcc gtgctgaact ggcgaagatg gaaccgttct tcccgtcggg tctgaaaatt
     6541 gtttacccgt acgacaccac gccgttcgtg aaaatctcta ttcacgaagt ggttaaaacg
     6601 ctggtcgaag cgatcatcct cgtgttcctg gttatgtatc tgttcctgca gaacttccgc
     6661 gcgacgttga ttccgaccat tgccgtaccg gtggtattgc tcgggacctt tgccgtcctt
     6721 gccgcctttg gcttctcgat aaacacgcta acaatgttcg ggatggtgct cgccatcggc
     6781 ttgttggtgg atgacgctat cgttgtggta gaaaacgtgg agcgtgttat ggcggaagaa
     6841 ggtttgccgc caaaagaagc tacccgtaag tcgatggggc agattcaggg cgctctggtc
     6901 ggtatcgcga tggtactgtc ggcggtattc gtaccgatgg ccttctttgg cggttctact
     6961 ggtgctatct atcgtcagtt ctctattacc attgtttcag caatggcgct gtcggtactg
     7021 gtagcgttga tcctgactcc ggctctttgt gccaccatgc tgaaaccgat tgccaaaggc
     7081 gatcacgggg aaggtaaaaa aggcttcttc ggctggttta accgcatgtt cgagaagagc
     7141 acgcaccact acaccgacag cgtaggcggt attctgcgca gtacggggcg ttacctggtg
     7201 ctgtatctga tcatcgtggt cggcatggcc tatctgttcg tgcgtctgcc aagctccttc
     7261 ttgccagatg aggaccaggg cgtatttatg accatggttc agctgccagc aggtgcaacg
     7321 caggaacgta cgcagaaagt gctcaatgag gtaacgcatt actatctgac caaagaaaag
     7381 aacaacgttg agtcggtgtt cgccgttaac ggcttcggct ttgcgggacg tggtcagaat
     7441 accggtattg cgttcgtttc cttgaaggac tgggccgatc gtccgggcga agaaaacaaa
     7501 gttgaagcga ttaccatgcg tgcaacacgc gctttctcgc aaatcaaaga tgcgatggtt
     7561 ttcgccttta acctgcccgc aatcgtggaa ctgggtactg caaccggctt tgactttgag
     7621 ctgattgacc aggctggcct tggtcacgaa aaactgactc aggcgcgtaa ccagttgctt
     7681 gcagaagcag cgaagcaccc tgatatgttg accagcgtac gtccaaacgg tctggaagat
     7741 accccgcagt ttaagattga tatcgaccgg gaaaaagcgc aggcgctggg tgtttctatc
     7801 aatgacatta acaccactct gggcgctgca tggggcggta gctatgtgaa cgactttatc
     7861 gaccgcggtc gtgtgaagaa agtttacgtc atgtcagaag cgaaataccg tatgctgccg
     7921 gatgatatcg gcgactggta tgttcgtgct gctgatggtc agatggtgcc gttctcggca
     7981 ttctcctctt ctcgttggga gtacggttcg ccgcgtctgg aacgttacaa tggcctgcca
     8041 tccatggaaa tcttaggcca ggcggcaccg ggtaaaagta ccggtgaagc aatggagctg
     8101 atggaacaac tggcgagcaa actgcctacc ggtgttggct atgactggac ggggatgtcc
     8161 tatcaggaac gcctctccgg caaccaggca ccttcactgt acgcgatttc gttgattgtc
     8221 gtattcctgt gtctggcggc gctgtacgag agctggtcga ttccgttctc cgttatgctg
     8281 gtcgttccgc tgggggttat cggtgcgttg ctggctgcca ccttccgtgg cctgaccaat
     8341 gacgtttact tccaggtagg cctgctcaca accattgggt tgtcggcgaa gaacgcgata
     8401 cttatcgtcg aattcgccaa agacttgatg gataaagaag gtaaaggtct gattgaagcg
     8461 acgcttgatg cagtgcggat gcgtttacgt ccgatcctga tgacctcgct ggcgtttatc
     8521 ctcggcgtta tgccgctggt tatcagtact ggtgctggtt ccggcgcgca gaacgcagta
     8581 ggtaccggtg taatgggcgg gatggtgacc gcaacggtac tggcaatctt cttcgttccg
     8641 gtattctttg tggtggttcg ccgccgcttt agccgcaaga atgaagatat cgagcacagc
     8701 catactgtcg atcatcattg atacaacgtg taatcactaa ggccgcgcaa gcggcctttt
     8761 ttatgcataa cctacgaaca ttaaggagta attgaaccac caactcagga tctcatacga
     8821 aaaccagtat taaccacgga taaaattcat aaaa
//
LOCUS       DAZGLJ010000181         8792 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000181 DAZGLJ010000000
VERSION     DAZGLJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.181"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..727
                     /locus_tag="ACHK49_003780"
     CDS             <1..727
                     /locus_tag="ACHK49_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFO9264348.1"
                     /translation="LPDNEMILKSVLAVKLVMQLKILNIVNKNFIENMKKTFSHCPYI
                     KDPIIRSYIHSGEDNKFDDFMRQHRFSKVDFDTQQMIHFINRFNMNKGLIDKNNNFFI
                     QLIDQALRSTDDMIKANAWYLYKEWIRSDDVSPLFIEIEDNLRTFNTNELTRKDNIFI
                     LFSSADDGPVMVVSSQRLHDMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSH
                     GKYALFPIFTASWRATRIKNIGI"
     gene            950..1447
                     /gene="ubiC"
                     /locus_tag="ACHK49_003781"
     CDS             950..1447
                     /gene="ubiC"
                     /locus_tag="ACHK49_003781"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO9264349.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1460..2332
                     /gene="ubiA"
                     /locus_tag="ACHK49_003782"
     CDS             1460..2332
                     /gene="ubiA"
                     /locus_tag="ACHK49_003782"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO9264350.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2487..4910)
                     /gene="plsB"
                     /locus_tag="ACHK49_003783"
     CDS             complement(2487..4910)
                     /gene="plsB"
                     /locus_tag="ACHK49_003783"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO9264351.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            5081..5449
                     /gene="dgkA"
                     /locus_tag="ACHK49_003784"
     CDS             5081..5449
                     /gene="dgkA"
                     /locus_tag="ACHK49_003784"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO9264352.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5559..6167
                     /gene="lexA"
                     /locus_tag="ACHK49_003785"
     CDS             5559..6167
                     /gene="lexA"
                     /locus_tag="ACHK49_003785"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO9264353.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6240..7565
                     /gene="dinF"
                     /locus_tag="ACHK49_003786"
     CDS             6240..7565
                     /gene="dinF"
                     /locus_tag="ACHK49_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO9264354.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7681..7890
                     /locus_tag="ACHK49_003787"
     CDS             7681..7890
                     /locus_tag="ACHK49_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO9264355.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7932..8447)
                     /gene="zur"
                     /locus_tag="ACHK49_003788"
     CDS             complement(7932..8447)
                     /gene="zur"
                     /locus_tag="ACHK49_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO9264356.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         2070 a         2083 c         2351 g         2288 t
ORIGIN      
        1 attgccagat aatgaaatga tattgaaatc cgttctggca gtaaaactgg ttatgcaatt
       61 aaaaattctt aatattgtta ataaaaactt tattgagaat atgaagaaaa catttagcca
      121 ttgtccttat ataaaagatc caattatacg cagttatatc cattctggtg aagataacaa
      181 gttcgatgat tttatgcgtc aacatcgatt cagcaaggtg gatttcgata cccaacagat
      241 gatccatttt attaacaggt ttaatatgaa taaagggctg attgataaaa ataacaattt
      301 ttttatccaa cttatcgatc aggccttacg atcaacggat gatatgatca aagcaaatgc
      361 ctggtatctt tataaagagt ggattcgtag tgatgatgtt tcacctctat ttatagaaat
      421 tgaagataat ttaagaacct ttaacacgaa tgaattaaca cgaaaggata atatctttat
      481 cctgttctcc tctgccgatg atgggccagt tatggtggta agctcccagc gcttacatga
      541 tatgttgaat cctacaaaag ataccaattg gaattccacg tgtatctata aatccagaca
      601 taagatgttg cctattaatc ttactcagga aacacttttt agctccaaat ctcatggtaa
      661 atatgcgctt ttcccaattt ttactgcgag ttggcgagct actcgtataa agaatatagg
      721 tatttaagta aaggaaaaca tcaccgttcc tggcatcatg ggcggtgatg cccctacggt
      781 tgccctcgcc agcacgggca tcggcaaagc gtaaggttca acagcggttt accacttcat
      841 gcgattgttg cgtttttgtt gcgcattaga tcacttaatt tgctttacat ctcccgtaaa
      901 cacttttctg cgatacaatg cctttacgtt atgtaacgga gagttcggca tgtcacaccc
      961 cgcgttaacg caactgcgtg cgctgcgcta ttttaaagag atccctgccc tggatccgca
     1021 actgctcgac tggctgttgc tggaggattc catgacaaaa cgttttgaac agcagggaaa
     1081 aacggtaagc gtgacgatga tccgcgaagg gtttgtcgag cagaatgaaa tccccgaaga
     1141 actgccgctg ctgccgaaag agtctcgtta ctggttacgt gaaattttgt tatgtgccga
     1201 tggtgaaccg tggcttgccg gtcgtaccgt cgttcctgtg tcaacgttaa gcgggccgga
     1261 gctggcgtta caaaaattgg gtaaaacgcc gttaggacgc tatctgttca catcatcgac
     1321 attaacccgg gactttattg agataggccg tgatgccgga ctgtgggggc gacgttcccg
     1381 cctgcgatta agcggtaaac cgctgttgct aacagaactg tttttaccgg cgtcaccgtt
     1441 gtactaagag gaaaaaaata tggagtggag tctgacgcag aataagctgc tggcgtttca
     1501 tcgcttaatg cgtacggata agccaattgg cgcgttactg ctgctctggc caacattatg
     1561 ggcgttgtgg gtggcgacac cgggcgttcc tcagctctgg atcctggcgg tatttgtcgc
     1621 gggtgtctgg ctgatgcgcg ctgctggatg tgtggtgaat gattatgctg accgaaagtt
     1681 tgatggtcat gttaagcgca cggcgaaccg accacttccc agcggcgcgg taacagagaa
     1741 agaggcgcgc gcactgtttg tagtgctggt actgatttcg tttttactgg tactgacgct
     1801 gaatacgatg accattctgt tgtcgattgc cgctctggcg ttggcgtggg tgtacccgtt
     1861 tatgaagcgg tatacccatc tcccgcaagt ggtgctgggc gcggcgtttg gctggtcgat
     1921 tccaatggct tttgccgctg tgagtgagtc ggtgccattg agttgctggt taatgttcct
     1981 cgccaatatt ctctgggcgg tggcttacga cacgcagtat gcgatggttg accgcgatga
     2041 tgatgtgaag attggcatta aatccacggc aatcctgttc ggccaatacg ataaattgat
     2101 tattggtatt ttgcagattg gcgtactggc actgatggcg atcatcggtg agttaaatgg
     2161 cttaggctgg ggatattact ggtcaattgt ggtggctggc gcgctgtttg tttatcaaca
     2221 aaaactgatt gccaaccgcg agcgtgaagc ctgctttaaa gcatttatga ataataacta
     2281 tgttggtctg gtactatttt tagggctggc aatgagttac tggcatttct gatgatgtaa
     2341 aaaagccgga tgatcatccg gctttcttct gggttgcctg atgcgcggcg cttctcaggc
     2401 ctacacaaca catcgcaatt tattgaattt gcagattatg gaaggccgga taaggcgttt
     2461 tcgccgcatc cggcaattct ctctgattac ccttcgccct gcgtcgcact ctcaatcgtc
     2521 aaacgcacgt ctgatgtaat caactccgcc agcaactgat aaaccttcat cgtttctgcc
     2581 ggttcggcat cgccgctatc gctgatatac ccttcatcac gcagtgtcag caccagagaa
     2641 ctgaacaccg ccttgtcgaa gaactccggc gcgttgatgc cgtgcagcac ggagagacgt
     2701 tgcgcgacgg tgcggctctc tttctccagc gtaccgcggt tgatcgacgg gttggcactc
     2761 aacaaccaga aggtgatggc ataacgttgc agcgtttcgc gcgcgcctgc ggcgagcagc
     2821 tgtagcgtgc gagaatgcac cgggttgata tgcaactcat catcttgcag ggtaatcagc
     2881 ccctgacgtt gcatctcatt tgccagcgca tcaataacgt ccggcaactc gtcgcgatcc
     2941 cagcgcagga acagctccgc tttcagcatt gggtaaagca cattgacgtg ctccatcaat
     3001 acgtcgcggg agatgtggcg atgctgggtg acaattgccg ccatcagcga aggcagcacc
     3061 aacatatgcg caatgttgtt gcgatagtag gtcatcagca ccgcttgctc gcgtggcaga
     3121 atgatgatgt cgccgatagt gtctttctcg acttcaaact tgttcatttg cagcgcgtga
     3181 tcgataagct cgctggcgct ggctgaagga acggtagagt ccgtggagta aggtacgttg
     3241 cgcatcagat ccaggtagca gttgagttgc tcggttaact gctcgcgggt gagtgagcgc
     3301 tgacgtgatg ccagtagcgc agtacagcac aggttcatgg cgtttgccgc gcctgcgttg
     3361 ttaatgcgta ccatcagatc ggcagcaata ttattgaccg tcggcgttaa ccaggccgga
     3421 cgcaccgctt cgatgggatc gatagattca cgccagtctg gtacgtgctg gttaaggtag
     3481 gtcatcaacg gcattggttc accgaagttg acgtaaccct gaccgagatt acgcagcttg
     3541 cttaaaccgc gcagcatctg cggcaggctc tctttctctt tcgtcgcgcc gcgcagttct
     3601 ttggcgtaag tacccacttc catgacgtgc tcataaccga tatagatcgg aatcagcgta
     3661 atcggacgcg tgccgccacg cagcatcgcc tgaatggtca tcgacagcgt accggttttc
     3721 ggatccagca aacgccccgt gcgggaacga ccgccttcca cgaagtactc gacggaataa
     3781 ccacggctga acagttcgcc gagatactca cggaaaacgg tggaataaag tttattgcct
     3841 ttaaaagtac ggcgaataaa gaacgccccc agacggcgga aaatcggccc ggccggccag
     3901 aagttcaggt tgatcccggc ggcgatatgc ggcggcacca gcccctggtg gtacagcacg
     3961 taagaaagta gcaggtagtc catgtgactg cggtggcaag gcacatacac cagctcatga
     4021 ccgtcgtggg ccagctggcg aacgcgctcg gcgttatgga cgttgatgcc ctgataaagt
     4081 cggttccagg tgaagcccag aatgcggtca gtcaggcgga tcatctcgta agagaaattc
     4141 gccgcaatct cttccatcag cgcaatcgcg ttctgctgcg ctttttcatg ggagattttt
     4201 ttgctgcgcg cttcatcttc taccgctttg gcaatggcgc gggaggcgag cagcttatta
     4261 aacagatcct gacgagcagg cagacgtggg cccacagcag ccagacgttg acgggcaaag
     4321 tgcatacgcg ccacgcgcgc cagtttctga gcgatagttt tatctgtgcc gtgttcatcg
     4381 gccatgcggc gcagagaaac tgacggcgag aaacgcacaa aactgtcgcg accgagccac
     4441 agtacggcga aaaatttttg tacgccgtta agcatacgca gcggcgggtt tacttcgcct
     4501 ttttcacgcc ccggcgcgcg accaaacatc accgatactg gcaccatctg cacatccaga
     4561 tttgggttgc tgcggtgcaa atcaagatag tcgtggaaca gcttaataga ctcttctttc
     4621 ggcgtgtaat aggtgaacac acgcggcccg ccgtgaatga acacatagcg cggcagtagc
     4681 gtgccgtcga tttccagcgg ctctaacggg tcaggcaagt catgagccag acactgggcg
     4741 cgcaacgtca gcaaatctgc tttcgagttg tacggtaaaa cgtacataat tggacgagag
     4801 gtatccagcc ccagttccgg ggcaggatct gccggaatag acttgctttt taccaggatg
     4861 cttaatggta aattcagtaa tttgtagtaa attcgtggcc agccggacat aaacgatgta
     4921 aagcctctgg ttaataatgc aaatgcgcgg caaggatagc agaaagtcat gggaaattct
     4981 gtggtatccg ctcatgtttc gcgcggcgtt acgcaaaccc gaatcatcgg atttaacggt
     5041 acactgatat tgacgctcat aatgtaaaaa ggttctttca atggccaata ataccactgg
     5101 attcacccga attatcaaag ctgctggcta ttcctggaaa ggtttacgcg ctgcatggat
     5161 caacgaagcg gcattccgtc aggaaggcgt agcggtattg ttggcggtgg tcatcgcctg
     5221 ctggctggat gtggacgcaa ttacccgcgt gctgcttatc agctccgtga tgctggtgat
     5281 gattgtggaa atcctcaata gcgccatcga agcagtggtt gaccgaattg gctctgaata
     5341 ccatgagctt tccggacgcg caaaagatat gggatccgcc gcggtgctga ttgccattat
     5401 cgtcgccgtg attacctggt gcatcctgtt atggtcgcat tttggataac ccttccagat
     5461 ttcgataaat ctctggttta ttgtgcagtt tatggttcca aaatcgcctt ttgctgtata
     5521 tactcacagc ataactgtat atacacccag ggggcggaat gaaagcgtta acggccaggc
     5581 aacaagaggt gtttgatctc atccgtgatc acatcagcca gacaggtatg ccgccgacgc
     5641 gtgcggaaat cgcgcagcgt ttggggttcc gttccccaaa cgcggctgaa gaacatctga
     5701 aggcgctggc acgcaaaggc gttattgaaa ttgtttccgg cgcatcacgc gggattcgtc
     5761 tgttgcagga agaggaagaa gggttgccgc tggtaggtcg tgtggctgcc ggtgaaccgc
     5821 ttctggcgca acagcatatt gaaggtcatt atcaggtcga tccttccttg ttcaagccga
     5881 atgctgattt cctgctgcgc gtcagcggga tgtcgatgaa agatatcggc attatggatg
     5941 gtgacttgct ggcagtgcat aaaactcagg atgtacgtaa cggtcaggtc gttgtcgcac
     6001 gtattgatga cgaagttacc gttaagcgcc tgaaaaaaca gggcaataaa gtcgaactgt
     6061 tgccagaaaa tagcgagttt aaaccaattg tcgttgacct tcgtcagcag agcttcacca
     6121 ttgaagggct ggcggttggg gttattcgca acggcgactg gctgtaacat atctctgaga
     6181 ccgcgatgcc gccaggcgtc gcggtttgtt tttcatctct cttcatcagg cttgtctgca
     6241 tggcattcct cacttcatct gataaagcac tctggcatct cgccttaccc atgattttct
     6301 ccaatatcac cgttccgttg ctgggactgg tcgatacggc ggtaattggt catcttgata
     6361 gcccggttta tttgggcggc gtggcggttg gtgcaacggc gaccagcttt ctctttatgc
     6421 tgttgctgtt tttacgcatg agcaccaccg ggctgactgc gcaggcttat ggtgccaaaa
     6481 atcctcaggc attagcccgt gcgctggtgc aaccgttgct gttggcgttg ggggctgggg
     6541 cgttaattgc gctgctgcgt acaccgatta tcgatctggc gctgcatatt gttggcggta
     6601 gtgaagcggt cctggaacag gcgcggcgct ttcttgaaat ccgctggtta agcgctccgg
     6661 cgtcgctggc gaatctggta ttactcggtt ggttactcgg cgtgcaatat gcccgtgcgc
     6721 cagtaatttt gttagtggtc ggcaatatcc tcaacattgt gctggatgtc tggctggtga
     6781 tggggctgca tatgaacgtg cagggcgcgg cgctggcgac ggttattgcg gaatatgcaa
     6841 cattgctgat tggtctgcta atggtgcgta aaatcctcaa actacgcgga atttccggcg
     6901 aaatgctgaa aactgcctgg cgaggaaact tccgtcgctt gctggcgctt aaccgcgata
     6961 tcatgctgcg ttcgctgttg ttgcaactct gtttcggcgc gatcaccgta cttggtgcgc
     7021 gactggggag tgacattgtc gctgttaacg cggttctgat gacgctactc acctttaccg
     7081 cctatgcgct tgatggtttt gcctacgcgg ttgaagcgca ctccggtcag gcatacggtg
     7141 cgcgcgacgg tagccagttg ctggatgtat ggcgggcagc gtgccgccag tcggggattg
     7201 tagcgttact gttttcggtg gtttatttgc tggcagggga acacatcatt gcgttgctga
     7261 cgtcgttaac ccagattcag cagctggctg accgctatct tatctggcag gtgattttgc
     7321 cgttggttgg cgtctggtgt tatctgctgg acggcatgtt tataggcgca acgcgtgccg
     7381 ccgaaatgcg taacagtatg gcggtggccg ccgcaggttt tgcgctgacg ctccttacgc
     7441 tgccgtggct gggtaatcat ggtttgtggc tggcattaac cgtctttctg gcgttgcgcg
     7501 ggctttctct ggcggctatc tggcggcgtc actggcgcaa cggtacctgg tttgccgcaa
     7561 cgtgacggtt aaaaattctg aataaataat cctaagccaa attgctgact acacttaatc
     7621 tcacgttcag aagaaaagtg aacgtactct cattcacaac ctaacgatga ggtcttgatt
     7681 atgaataaag atgaagccgg cggtaactgg aaacagttta aaggtaaagt gaaagagcaa
     7741 tggggcaaac tgaccgatga tgatatgacg atcattgaag gtaaacgtga tcaactggtc
     7801 ggtaaaatcc aggaacgtta tggttatcag aaagatcagg cagaaaaaga ggtcgtggat
     7861 tgggaaaccc gcaatgaata tcgctggtaa ttaatccctc ctgcccgacg tgtacaagga
     7921 tgtacgccct cttaacgcgg tttctttttc acctgcacag agtgatcatg ctggcactgt
     7981 tcaggatgac gacacgcttc cacttccacg catgccgcac agagcccatg tgcttcaatc
     8041 acattatgcc gcagggcaaa ccccattttt gccgccagcg tatgcataat gtcttccacg
     8101 ccttctgcac actcttcttt caccgcgccg cagcgatcgc aaataaacat ggctgacgta
     8161 tgggtgggct ggtcgaacag atgacagagc acataactgt tggtggattc caccttatgc
     8221 acaaaacctt gctcaagcag aaaatccagc gcgcgataaa ccgttggcgg cttggcttgc
     8281 ggttcagctt cgcgcagtaa atcaagcaga tcataagcgc tgatagcgcc atcctgcaga
     8341 ctcatcaggc gcaacacttc caggcgctgt ggggtcaggc gcacattacg ctgcgcgcag
     8401 attttttcag cctgcgctaa taactcctgc gttgtggtct tttccatcgg gcacctcaaa
     8461 gtgggggtta agaatgtcct tactttacca tgttccagga aaaacaccga gaacccttat
     8521 tgttgccgta atgttgattt tctgttttgt aggtaaggtg ttgtgttgcc ttgtcgtacc
     8581 attatcaaca cgataataat taataattca tttttaaata aattgcttca agttaactcg
     8641 ctatttattt aactgggttt aaacgcatac ctgtagttca gaaattatga gcgatttttg
     8701 atagtgacca gatggcaaaa gaattataag gaggacacgg tacgacaaaa aaggtagatg
     8761 aatcatgttg aagataatac ccggtgcaac tg
//
LOCUS       DAZGLJ010000182         8689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000182 DAZGLJ010000000
VERSION     DAZGLJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.182"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1324
                     /locus_tag="ACHK49_003789"
     CDS             <1..1324
                     /locus_tag="ACHK49_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO9264357.1"
                     /translation="LSDNYNAITLGNGWNTPLGAISFYAARSSSKLNNDTRHEGTSYQ
                     VAYNKYLVQTATRFSVAAWRYASQDYRTFSDHLYENDKHYHQSDYDDFYDIGRKNSLS
                     ANIMQPLSNNLGNVSLSALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSYDENN
                     KEEERFNLFISIPFYWGDDIAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLN
                     YGIYVNQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGIN
                     ITNQLSDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSES
                     ETELQGNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIG
                     VVGQGSRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            1337..2296
                     /locus_tag="ACHK49_003790"
     CDS             1337..2296
                     /locus_tag="ACHK49_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFO9264358.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECTDLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGKSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(2335..3234)
                     /gene="nhaR"
                     /locus_tag="ACHK49_003791"
     CDS             complement(2335..3234)
                     /gene="nhaR"
                     /locus_tag="ACHK49_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO9264359.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(3300..4466)
                     /gene="nhaA"
                     /locus_tag="ACHK49_003792"
     CDS             complement(3300..4466)
                     /gene="nhaA"
                     /locus_tag="ACHK49_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO9264360.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5052..5204
                     /gene="mokC"
                     /locus_tag="ACHK49_003793"
     CDS             5052..5204
                     /gene="mokC"
                     /locus_tag="ACHK49_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO9264361.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(5308..6438)
                     /gene="dnaJ"
                     /locus_tag="ACHK49_003794"
     CDS             complement(5308..6438)
                     /gene="dnaJ"
                     /locus_tag="ACHK49_003794"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO9264362.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(6527..8443)
                     /gene="dnaK"
                     /locus_tag="ACHK49_003795"
     CDS             complement(6527..8443)
                     /gene="dnaK"
                     /locus_tag="ACHK49_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO9264363.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
BASE COUNT         2323 a         2120 c         1960 g         2286 t
ORIGIN      
        1 tttatccgat aactataatg ctataactct gggaaatggt tggaatacac cgctgggtgc
       61 catctctttt tacgctgccc gatcgagtag taagttaaat aatgatacca gacacgaagg
      121 tacgagctat caggtcgcct ataataaata tctagtacaa acagccactc gatttagcgt
      181 ggccgcctgg cgttatgctt cgcaggatta caggacattc agcgaccatc tttacgaaaa
      241 tgataaacac tatcatcaga gtgattatga tgatttttat gatattggca gaaaaaatag
      301 cctttctgct aatattatgc aacctttatc caataatctg ggaaatgtat cattaagtgc
      361 gctttggcgg aattactggg ggcgaagtgg aaatgctaaa gattaccaat tcagttactc
      421 caataactgg caacacatta gttatacttt ctctgccagc caatcttatg atgaaaataa
      481 taaagaagag gagcgtttta atctgtttat ctccattcct ttctactggg gggatgatat
      541 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
      601 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      661 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      721 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      781 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      841 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg
      901 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
      961 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg
     1021 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
     1081 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
     1141 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
     1201 ttttattcgc gtagatgaaa ttcccactgg cataaaagtt gctctcaatg atgaacagaa
     1261 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
     1321 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
     1381 tgtgcaaagt gctccctgtg cattgaccaa tgtcggtgaa caacgtggaa catatatact
     1441 caagccactg agtatgaaag gtaatctaac cgccgaaaaa attgaaaaca ccggaaatat
     1501 cattgagtgt acagacttgc aagagacaac caacgccatt aaagttcatt ttttaacaca
     1561 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     1621 tgacggtaaa aaaatagagc ttccagggaa gtcatcttat aatttagaag aaatgctatc
     1681 tgcgataaat ttcaccattc catattctat cgcatctgta tcagatagtt ctaacaccgc
     1741 agacattttt cctggaactc cttttccttt gttgttcgaa attgaagtga gttcatgtac
     1801 agggaataat actaattgta atagcgtccg ctttaattat aatttaaata ctattttaca
     1861 ggtaaattta atgacctgtg gttttgaaga tcaatccatc gacactggac gatttgtact
     1921 gtctcgtatc aataatgaaa attatcagtt tcatcatata agatttgact gcatccaggg
     1981 cgagcaaggt gaattagttt ttgaacccag tgatgttgaa tattactttg aaccagtgac
     2041 gatacaggag tcaggaacca gcatattaaa aaatgagtta gaaaacagtg aggacggtgc
     2101 gggtcaaata ggctttcaac tctccaatga tggtacgaat gaaatccagt atggtaaaag
     2161 caattactac tcttttcagc atccccatga aggaagtaat cagattcctc tatttattcg
     2221 cccgcgtaca tatggaaata atgtctcaag cgggcaaata atgagccgag tcaaaatcgt
     2281 ggttatgtac aactaatcta tctcccccac cttaactttg gggggaggtg ccgattaacg
     2341 cgccgctgga ctaaaaagcg cagaataatc cgtattgcag attcgctgta ccgccgggtg
     2401 ctgaatcatc cgctcagcaa aaatagcatg gtactcttcc atcacattct cgacgcgacc
     2461 aatttctacg acagttttat cggcataaaa gtcatatgca taaagcgttg gggcaacgaa
     2521 gattgcattg tgcatcgcac caaaagcttt catcaacgcg gcatcatcaa actcgccgag
     2581 gatttctacg tttaatccct gggagttaaa ccagttaagc aatttgcgcc ctaacattga
     2641 acgtcgccca ggaatcaaaa gtcgccgttc ttccagacaa gccgggaacg gtttttctgg
     2701 tggtggattt gtacaccaga aactcacgcc acattcgcca attctcacgg agaacaggcc
     2761 ttcctgctgc gtagagtcta tcggacagtc agaaatgatc atatccagtt tatgctgact
     2821 taattgctcc agcagcattt cgtgggtgga ttcgaagcag cgaagatgaa tgggctcgcc
     2881 ttctaccact gcggcgttaa gtacgctact gaccaggcgt ttggaaagtg catcagccac
     2941 accaacgtca aacaataaat tggattcttt gcgatagttc acaatatcca gcatttcctg
     3001 gcttaaggtg aacattttat cggcatagcg atagaccagt tctcccagct cgctgggttc
     3061 gagaccacgt cccttgcgtt taaataattt gccttgcagg cgctcttcca gcgctcgaat
     3121 ctgtccggta atggtttgtg gagttaaata aagcgcctcc gctgcgccaa ccacggaacc
     3181 ttctttatag acatgccaga agtaatacaa gtggttgtaa ttgatatgag acatgctcat
     3241 ttctctccct gataacaatg aaaagggagc cgtttatggc tccccggtaa accgtcctgt
     3301 caaactgatg gacgcaaacg aacgcgtaac cagctgtatc caattaccgc cgaagagata
     3361 gaaccgacca ggataccgag tttcgcccag ttaatcagtt ctggatctac gctaccaaag
     3421 gccaggctgg caataaagat agacatagta aaaccgatac cgcacaggat ccccaccgcc
     3481 ataatttgct gataagtcgt tccctcaggc agatgcgcca gtttcaaacg cagcgccaac
     3541 cagcagaaca gactaatccc cagcggtttg ccaatcagca agccagcgat gatccccaat
     3601 ggcagaatgg aggtcaagcc atccagcgtg acaccttgca gtgaaacgcc agcattagca
     3661 aatgcaaaca gcggcaaaat cagatacgcc acccacgggt gtaacacatg ctccagtcgc
     3721 ttcgctggag aacgcccatg cttctctttc aaaggaataa agaagccgac aattaccccc
     3781 gccagggttg cgtgaacccc cgatttcaac accgcagtcc acaacactac gccaaccaga
     3841 atatagacgc ccgtgcggcg tacaccacac agattcaata ccgcgagtac cgcaattgct
     3901 acagccgcga cgccaagaga ggccatcgat aagtcattag tgtagaacaa tgcgatgata
     3961 atgatggccc caagatcgtc gataatagcc agagccatca aaaagatctt cagcgctaac
     4021 ggaacacgac ttcccaacag cgccagcaca ccaagtgcaa aggcaatgtc agtagccgcc
     4081 gggatcgccc acccttcgcg ggtaatcgga tcggcatagt taaaggccag atagagtaat
     4141 gccggaacaa tcatcccacc aatagcggcg ataactggaa atgcggcctg gcgtaagctg
     4201 gctagcgatc cttgcatcag ttcacgttta acttccagac cgactaacag gaaaaatacc
     4261 gccatcagcg cgtcatttat ccataacagc atgtttttgt tgatttcgag tgaaccaacc
     4321 cggagctgaa ccggcgtctc cagaaagtcg tgataccatc cactggttgc gccgctattg
     4381 gccataatca tcgccaggat agcggcaatg ataagaataa tgcctcccga ggcatcactg
     4441 ctaaagaatc gatgcagatg tttcactttt tatttctctt tcaggtgaat agatcgtttt
     4501 tactatttta ccccgcacaa atcatcgtta aaatcagatt atagtgtagg aataactcgt
     4561 tttttacgat ccattgaaga atttgcgcca tcagcggtga gatgagtttt tgacggctgt
     4621 aagcgctcac gagtgaccct aagcccagat aatcatagct taaggtgagg atttctgagg
     4681 gattaattag gaaaagtaat aattaatttc ttgtcagtcg gcgttatttg ttaggacaat
     4741 atttcaaaaa taatccagct tgtcgtcaat aattgcgata aagagataaa ttaaagttaa
     4801 ataattaaat cattaaaaaa ttacgccaca aaaatgcgag gcgtcttcag gattagaatc
     4861 gataaaacag aatgggttaa agagagcgat ataatagcgg cgggtacttg aggctatctg
     4921 tctcaggcat tagctgaacg gcagatagag aaaagccccg agtgatattt tactatcaac
     4981 ccgaggcccc ctatatgctg aacacatgta gagtgcctct tactgaccgt aaggtcaagg
     5041 agaagagagc aatgaagcag cataaggcga tgattgtcgc cctgatcgtc atctgtatca
     5101 ccgccgtagt ggcggcgctg gtaacgagaa aagacctctg tgaggttcac atccgaactg
     5161 gccagacgga ggttgctgtt ttcacggctt acgaatccga gtaagtgcaa cggcggggag
     5221 tgatccccgc cacttttcag gtgctcgcat atcttcaacg caccctaatt ttacccaagc
     5281 ctgcccacgg gcaggctttt ggggaggtta gcgggtcagg tcgtcaaaaa acttcttcac
     5341 accatcaaag aagctctttg agcgcgggct gttgtgctca ccggttgggc caccgaagct
     5401 ttcttgcagc tcttgcagca gctgtttctg cttctcgttc aggcctaccg gtgtttcgac
     5461 gacaacgcgg cacagcaaat caccctgtgc gccaccgcgg acagacttga cgcctttacc
     5521 gcgcatacgg aaaagcttac cggtctgggt ttcgccaggc actttcagtt tgacgcgacc
     5581 atcaagggtc ggtacttcga tttcgccacc cagcgccgcc atagcgaagt tgatcgggac
     5641 ttcgcaatag aggttgttgc cttcacgctc gaaaatcggg tgctgtttaa cctgaacctg
     5701 aacgtacaga tcgcctgccg gtgcgccatg ctcgcccgct tcaccttcgc ccgcaagacg
     5761 gatgcggtct ccagtgtcca cccctgccgg gattttaacg gacagcgttt tgctgcgctc
     5821 aacacgacca tgaccatgac atttgttgca cggatctttg atcagcgtac cgcggccctg
     5881 acagtgtgga caggtctgct gtacagcgaa gaatccctga cgcatctgca cctgaccaga
     5941 accatgacag gtcggacagg tctgcggctg tgtacctggt tttgcaccgc taccgtggca
     6001 aacgtcacac tcttccagag tcggaatgcg gatctctttg gtcacgccac gtacagcttc
     6061 ttcgagggtg agctccatgt tatagcgtaa atcagcaccg cgcgccgcac gttgacgacc
     6121 acgtccgccg ccaaaaatat cgccgaaaac gtcaccaaaa atatcgctga agtctgcgcc
     6181 gccgccaaaa ccgccgccgc ccataccacc ttgctcaaac gcagcatgac catactgatc
     6241 gtatgccgca cgtttttgcg agtcggtcag aacttcataa gcttccttga tctctttaaa
     6301 tttcgcctcg gcctctttgt caccctggtt acggtccggg tggtatttca tggccaggcg
     6361 tttgtaggcc tttttgattt cacgctcttc cgctgttttg gaaacgccta aaatctcgta
     6421 ataatcttgc ttagccatct tttttaaatt gcccctagat gaatgcacgg gcggagagga
     6481 aattcccctt cgcccgtgtc agtataatta cccgtttata gggcgattat tttttgtctt
     6541 tgacttcttc aaattcagcg tcgacaacat cgtcatcttt cgcgttgttt gcagaagcat
     6601 cagcaccggc agtctgctgc tgggcatgtt gctgctgggc gatttccatc agtttctggg
     6661 aaacctgtgc cagctcctgc attttcgctt cgatagcggc tttgtcttca cctttcagag
     6721 cagtttccag tgcagtcagt gcagactcga tagcagtttt gtcgtcagcc ggcagtttgt
     6781 cgcctgcttc ttcaacctgc ttacgggtgc tgtgcagcag atggtcgccc tggttgcgag
     6841 tctgtaccag ctcttcaaac ttacggtcag cttcggcgtt agcttctgcg tcgcgtacca
     6901 ttttctggat ttcatcttcg ttcagaccag aagaagcctt gatggtgatc ttctgctctt
     6961 taccgctgtt tttatctttc gcggaaacgt gcaggatacc gtcagcatcg atatcgaagg
     7021 taacttcgat ctgcggcatg ccgcgcggtg ccgggttgat accatccagg ttgaactgac
     7081 ccagagattt gttatcagcc gcacgtttac gttcaccctg cagcacatgg atggttaccg
     7141 cagactggtt gtcttcagcg gtagagaaca cctggctgtg cttggtcggg atagtggtgt
     7201 ttttcgcgat cagcgtcgtc atcacaccgc ccatggtttc gatacccaga gacagcgggg
     7261 taacgtccag cagcagtacg tctttcacgt caccagtcag aacaccaccc tgaacagcag
     7321 caccgattgc tacagcttcg tccgggttaa cgtctttacg cggctcttta ccaaagaact
     7381 cagcaacttt cttctgaacc attggcatac gagtctgacc accaacgagg ataacgtcgt
     7441 cgatatcaga tacggacagg ccagcgtcct gcagtgcaac tttcagcggc tcaatggaac
     7501 ggtttaccag atcttcaacc aggctttcca gtttcgcacg agtcactttg atgttcatgt
     7561 gtttcggacc ggtcgcatct gcagtgatgt acggcaggtt aacgtcggtc tgctgagcgg
     7621 aagacagttc gattttcgct ttttctgccg cttctttcag gcgctgcatt gccagcggat
     7681 cgttgcgcag gtcaatgccc tgatctttct tgaattcttc aaccaggtag ttgatcagac
     7741 ggctgtcgaa gtcttcacca cccaggtggg tatcaccgtt ggtagccaga acttcgaagg
     7801 ttttttcgcc gtcaacttcg tcgatttcga taatagaaat atcgaaagta ccacccccca
     7861 ggtcataaac cgcgatagta cggttgccag tacctttgtc cagaccgtaa gccagcgcag
     7921 ctgcggtcgg ttcgttgatg atacgtttta cttccagacc agcgatacgg cctgcgtctt
     7981 tggttgcctg acgctgagca tcgttaaagt atgccggtac ggtgataaca gcttcagtta
     8041 ccggttcacc caagtaatct tcagcggttt tcttcatttt tttcagcact tcagcagaaa
     8101 tctgcggcgg tgccattttc tggcctttaa cttcgaccca tgcgtcgccg ttatcagcag
     8161 caataatttt gaacggcatg atggaaacat cacgctgtac ttcttcgtcc tggaagcggc
     8221 ggccaatcag gcgtttaatc gcaaacaggg tgttttgcgg gttcgtcact gcctgacgtt
     8281 tagccggctg accaaccaga gtttcaccat cctgggtata ggcaataata gaaggcgtgg
     8341 tgcgatcgcc ttcggcgttc tccagcacgc gaggagtggt gccatccata atcgctacac
     8401 aagagttggt agtacccagg tcgataccaa ttattttacc catctaaacg tctccactat
     8461 atattcggtc atcatgtggt tgtgagtctg taataggggc gaaacgtctg gtttcaactg
     8521 cccaatttca tttttttgca gactcactca ctgcggttga ctactaaatg gggtcgtaac
     8581 ccacgtcatc aagggggaga tgcaaaaatt ttttgtgctt ttgcgcagaa atttcgcgat
     8641 atagcaggac aagacgtgaa actgacagca attttattca tataaagaa
//
LOCUS       DAZGLJ010000183         8617 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000183 DAZGLJ010000000
VERSION     DAZGLJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.183"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(54..815)
                     /gene="kduD"
                     /locus_tag="ACHK49_003796"
     CDS             complement(54..815)
                     /gene="kduD"
                     /locus_tag="ACHK49_003796"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO9264364.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(845..1681)
                     /gene="kduI"
                     /locus_tag="ACHK49_003797"
     CDS             complement(845..1681)
                     /gene="kduI"
                     /locus_tag="ACHK49_003797"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO9264365.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1968..3149)
                     /gene="yqeF"
                     /locus_tag="ACHK49_003798"
     CDS             complement(1968..3149)
                     /gene="yqeF"
                     /locus_tag="ACHK49_003798"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO9264366.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3404..4633
                     /gene="yqeG"
                     /locus_tag="ACHK49_003799"
     CDS             3404..4633
                     /gene="yqeG"
                     /locus_tag="ACHK49_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9264367.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            5093..5725
                     /gene="yqeH"
                     /locus_tag="ACHK49_003800"
     CDS             5093..5725
                     /gene="yqeH"
                     /locus_tag="ACHK49_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9264368.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQY"
     gene            6059..6868
                     /gene="yqeI"
                     /locus_tag="ACHK49_003801"
     CDS             6059..6868
                     /gene="yqeI"
                     /locus_tag="ACHK49_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9264369.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
     gene            6861..7343
                     /gene="yqeJ"
                     /locus_tag="ACHK49_003802"
     CDS             6861..7343
                     /gene="yqeJ"
                     /locus_tag="ACHK49_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFO9264370.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(7376..7456)
                     /locus_tag="ACHK49_003803"
     CDS             complement(7376..7456)
                     /locus_tag="ACHK49_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264371.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(7492..7917)
                     /gene="yqeK"
                     /locus_tag="ACHK49_003804"
     CDS             complement(7492..7917)
                     /gene="yqeK"
                     /locus_tag="ACHK49_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFO9264372.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKSSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
     gene            8111..8601
                     /locus_tag="ACHK49_003805"
                     /pseudo
     CDS             8111..8601
                     /locus_tag="ACHK49_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2605 a         1902 c         1691 g         2419 t
ORIGIN      
        1 caggtataaa aaaccctgcc atgcggcagg gtcataaaag taagaagaat gaattaacgc
       61 gccagccaac cgccatccac ggcaatggta taaccattca cataatctga agcgctggag
      121 gcaaggaaca ctatcggccc catcaggtca ctcggcagtc cccaacgacc agctggaatg
      181 cggtcgagaa tttccgcgct acgttgttca tctgcccgta gttgttgagt attgttggtc
      241 gccatgtaac ccggagctat cgcattaaca ttaatgttgt gtttagccca ttcgttcgcc
      301 atcaatcgcg tcacacccat cacgccgctt tttgatgcgg tataagaagg cacacggatc
      361 ccgccctgga aggagagcat tgacgcgata ttgataatct tgccgccatt gccttgcgcg
      421 ataaagtgtt tcgccgctgc ctgagacatg aagaatacgc tcttgatatt caggttcatg
      481 acatcgtccc agtccttttc gctgaactcg agagcatctt cgcggcgaat caatccggcg
      541 ttattcacca ggatatcaat atgaccaaac tccgctaccg cgcgatccag cagtccagga
      601 atgccatcaa tctttcgcag atcggcggtc aggcttaaaa aacgacgccc cagcgccgtg
      661 acctgctcga tggtttcagt cggttcaacg atgttaatgc caacaatgtc acagcccgct
      721 tgcgccagcc ccaacgccat cccctggccc agcccagtat cacaaccagt gacgaccgca
      781 actttacctt cgagagaaaa tgcacttaaa atcataacaa taccttattc gtttatgccc
      841 acaactagcg caaatcttta acggccacat ggtccatatc atcaaagacc tggttttcac
      901 cgaccatgcc ccagataaag gtataagctt tggttccgac gccggaatga atcgaccagc
      961 tcggggagat caccgcctgc tcgttatgca tcacaatgtg acgcgtttct tgcggctgcc
     1021 ccatcatgtg gaaaacgcag gcgtcatcat ccatattgaa atagaaataa acttccatcc
     1081 ggcgctcgtg agtgtgacac ggcatggtgt tccacaagtt acctggagcc agctccgtca
     1141 gccccatact caattggcag gtttccagca catccgggac aaaatatttg ttaattgtgc
     1201 gacggttact ggtgaggtta tcgcctaacg tgactggaga tacttcgtcc ggtgtgactt
     1261 ttttggtggg atacgtcgta tgcgcgggtg cgcaattgta ataaaacttt gccggagtgg
     1321 cggtatcaat actggcaaag acaacttctt ttgcaccttt acccacatac agggcgtcgc
     1381 ggtgaccgat ttcatagcat tggccatcga cagtaatcgt accggcaccg ccaatattga
     1441 taacacctaa ctcgcgacgt tcaaggaaat agcttacgcc gagttgttta ccaacttccc
     1501 cgccaacgga aaccgtttta gttatcggca taatgccgcc aacaataatt cggtcaatgt
     1561 ggctgtaaac catggtgtac tcatcggcga caaatacctc ttcaaccaaa aattcattgc
     1621 gcagcccttg ggtatccagc gtttttgcgt gcgcactgtg gatgctctgt cttacgtcca
     1681 catcaacctc cgataatcag tgaagcgtga aaatagaaaa cgatagtgaa acactgtttc
     1741 gttttctcta taaagggatg atatgcccat gggctagggt tttcaattaa aaataaaaca
     1801 atgtttcatt tttaagttaa gaattaaaaa aagtgcagtg tcgatcacga atatgtcatg
     1861 agaatggaaa aatgagggag cccagaaaat gtatgtcgac ttgccagcca tcattattca
     1921 acagattaat gacggcagat atttatcggt aaggaggaaa tgcagagcta ctcgtcacgt
     1981 tcaatggtca atgccacgcc ctggcccccg ccgatacaaa gcgttgccag ccctttgcgg
     2041 gcattacgtt tcaccatttc atgaaccaga gaaaccagga ttcggcaacc ggaagcgcct
     2101 atcgggtgac cgagtgcgat cgcgccacca ttgacattga cccgacgctc atcccactca
     2161 agcatcttgc caaccgaaag cgcctgtgca gcaaacgctt cattagcctc gataagatcg
     2221 acatcagcca actgccagcc tacacgctcc aggcaacggc gggtcgcata caccggcgca
     2281 attcccatca atgccggatc tacaccaacg ctggcaaatg cgcggatgcg ggccagcacg
     2341 ggtaaattca acgctcgtgc tttggcttcg ctcatcatca ttaccgcagc tgcgccatcg
     2401 tttatggatg atgcattccc cgcggtcaca gaaccgagac tatcaaatga aggatttaaa
     2461 cgggctaaac cttctgcact ggcgtcagtg cgtggctgtt catcggtatc aacaaccaac
     2521 gtctgaccgt tactttggat cattaccggg acgatctcat ctttaaatcg tccggcgtca
     2581 atcgccgctc gcgctttttg ttgcgagcta agtgcgtaag catcctgcaa ctgacggctg
     2641 atgccatatt cgcgagccag attttcggcg gtgacaccaa tatgataatc attgaaggca
     2701 tcccacaacc catcatgcac cagactgtca accaactggc tattgccaag ctgtgcaccg
     2761 gtgcggctat cagtcagaac atgtggtgcg cggctcatgt tttcctggcc accggcgatg
     2821 acaatatcag cctcgccaca ctgtatcgcc tgagtagcca gatgcagtgc tttaagccca
     2881 gaaccgcaaa cgtcattaat agtgattgca gaaacgctat taggaagacc acctttaata
     2941 gccgattgcc ttgccggatt ctgccctgcc cctgcagtca acacctgacc aagaattact
     3001 tcatccaccg catatgcagg aacgccggta cgttctatta acgctttcac gaccagacta
     3061 ccaagttcca cggcggaatg acccgctaac gcaccgcgaa agcagccgat aggtgtccgt
     3121 aacgccccga caatcacaac gtctttcatc acaacctcgc accaattaac agtgcaatag
     3181 taaatgattg ttaattacag ttatcttaat tgtttaaaaa aagtgatttt tatcacaaag
     3241 gaaatatgcc tgagcagtag tcagagtcat aactggcacg taaggtttgc aaccactaac
     3301 ccaccaatag aggggtagat agggcgttaa tctcccatac ttaacctggt ttatggtaaa
     3361 ttgccctcca ttttgtttaa tttgtagatg atacgttcag ataatgtcta atatttggtc
     3421 taaagaagaa actctgtgga gtttcgcgct ctacggcaca gccgttggtg caggcacgct
     3481 cttccttcct attcagttag gttcggcagg ggctgtggtc ctgtttatta ctgctctggt
     3541 cgcctggcct ttaacatatt ggccacataa agccttatgc cagttcatcc tctcatcgaa
     3601 aacatcagca ggtgaaggga taacgggcgc agtaacacac tactatggca agaagattgg
     3661 taatctgatt accacgctgt acttcatcgc cttttttgtc gtcgtgttga tatatgcagt
     3721 ggcaattacc aactcactta cggaacagct ggcaaagcat atggttattg atcttcgcat
     3781 ccgtatgttg gtgagtctgg gtgttgtatt aatcctgaat ctcatttttc tgatgggacg
     3841 ccatgcaacc attcgggtaa tgggtttttt ggtattccca ttgattgcct atttcttatt
     3901 tctttccatt taccttgtcg gtagttggca acctgatcta ttaacgaccc aggtagagtt
     3961 caatcagaat acccttcacc agatatggat atcgattccc gtgatggttt tcgcctttag
     4021 ccatacgccc attatttcta cgtttgccat agacagacgt gaaaaatatg gcgaacacgc
     4081 tatggataaa tgcaaaaaaa ttatgaaagt cgcttatctc atcatctgca taagtgtact
     4141 gttctttgtc tttagctgcc tgctttctat tccaccttcg tatattgaag cggctaaaga
     4201 agaaggggtc accattttat cggcgctttc tatgctgccg aacgccccag catggctgtc
     4261 aatttccggg attattgtcg cagtagttgc gatgtcgaaa tcattcctgg gtacgtactt
     4321 tggcgttatt gaaggtgcca cggaggtcgt caaaacaaca ctacagcagg ttggtgtaaa
     4381 gaaaagtcgt gcatttaacc gcgcactatc aattatgttg gtatcgctga tcaccttcat
     4441 tgtttgttgc attaacccga acgcgatttc gatgatttac gcgatcagcg gcccgctcat
     4501 tgccatgata cttttcatca tgcctacgtt gtcaacgtat ctgatcccgg cgcttaaacc
     4561 ctggcgttcc atcggaaatc tgattacgct gatcgtgggt atcctgtgcg tatcggtaat
     4621 gttctttagc tgatatccat tgtgaatggt tcaaaattgc tacaattttg aaccattcca
     4681 gttttcataa tgttcattcg ttattattcc gcactatcac ttccctcatc atatcctagt
     4741 attatcagtt agttaaacac acaaagagat ctgaacatct taaataagac tatttaagat
     4801 gcataactta gattcgcaag atatcttccg gtcattcaaa aacaatttgc gatatagata
     4861 ttctcatacc atgagatagt tatctgaacc accctcaacc aggtaagatt aaatcagtga
     4921 aacataatca tatttaataa cgagaaatgc attttaaaat gtcttaatca gaattatagc
     4981 aaatacagat taatcgcaat ttttcccagc aaaattacaa aatcatggtg tattgacatc
     5041 atcaatcacc aatgaaatta tgcaattata tacggatagg gaggttctta acatggggct
     5101 ttgtagtcgt tataaaagtc ttacatgcaa tagttgctca atgcattgcc aaataatgcc
     5161 agaagagtca ccgcgtttgc agtactgcgc aaactcgtgt ttttgtatgt ggcccgaaga
     5221 aagttcatat tttaatcgtg gtgtggtaga aggtatctta acaaaaaacc acaatgcgag
     5281 attaagcgga tatatttttg tcgatttttc agtaagtttt ttacgtctat tccttgaaaa
     5341 agactggatt gactatcttg caagtactga catggggatt gttttagtca gcgaccgcaa
     5401 tatgcaatcg ttagctaatt actggcgaaa acacaactca gcaatatctg ctgtcatata
     5461 taatgatgat ggtcttgatg ttgccaatga aaagatcaga caactattta ttggtcgtta
     5521 tttatcattt accagaggta acacattaac tcagatggaa ttcaccataa tggggtatat
     5581 ggtttctggt tataatccat atcagattgc tgaagttctg gatatggata tccgtagcat
     5641 ctacgcgtac aagcaacgaa tcgaaaagag aatgggtggt aaaataaacg aattatttat
     5701 tcgttcacat tcggtccaat attgatactc aacgaccagc cagaatcata ctctgtttat
     5761 acgggcattt attgaaatgt ccgtatttta taaataggcc gtcccctcaa gagtaaacac
     5821 catagtattt aaaaccatca cacgaaaaat tcagaagcat tacgaaatat gaatttacaa
     5881 acaatagtgg cataaatgtt aaccatgtta atttacgtaa agttttacgt tgcaacatta
     5941 aagcctcatt tcaatcatca tgataaatat aaaattaata tatatttatg ccgtaaatac
     6001 caatatactt agcaaactat gtgatctcca tttcgattga tttagtgttt attgacgtat
     6061 gtactggatt attaacgata atatcgagtt ctggcctgag caccgaaaat taatatcggt
     6121 acataacgcc gatcttaacg tcgttctgac aacgccagcc agtcgatgtt tatcacttct
     6181 acttgaagct tttcctgatg tggttgcaca acaagatttt ttcaccagag tctgggaaga
     6241 agaaggtatg cgtgtgccta ctaacacgtt atatcagaac atatccatta tcagacgcgg
     6301 atttcgcgct gttggtgata ctacccactc gctaattgca accgtgccga gaagaggatt
     6361 caagatccat aatgacatta acatacaaaa tcatgtaata aactcgtcaa cagacgcaca
     6421 tacacacaat gccccacctg ccataaaagt taatgcggga tacaaagaga gcattggtgg
     6481 cgcaaagaat ttcaataaca aaatcctcaa acatataaaa agtcatctaa ttatgttgag
     6541 cgcatttgtc ataggcgcat attctgcata ttggctatgg aataataatc aaccgaagcc
     6601 atttttcaag gattacaaga ctgttgcgga aattaatggc tgccatttta atgtaacaga
     6661 agatacaatt gacgggttga aagagttcga taaatataaa acacggatac tggattcggg
     6721 gattaactgt aaaaaacatc cgtggttata cttccctctt gcaaaatcct caccggggat
     6781 gattgttatg gcatgcaata aaaattataa ccaacatgaa gtggcagact gtttaaccct
     6841 gtcttaccgt gaggttaatc gtgattgatt ataaaaaaaa cttattattt attttagttt
     6901 ttatcagtgg gtttattctc ttcactgtct attcttacac tgcagaaaaa atgatatata
     6961 acgaaacatg cactgcaaat tgggtaattt ttaatgacca gggacgagca aatcttacca
     7021 tcgactttat gtataacaaa aaaaataaaa caggaactgt tgcgttgagt ggaacctggc
     7081 aacaaggtaa cagagaaagt aaatcaatac ggcgaaatat tgaatacaca tggattgaaa
     7141 actatgacac agcccattta acatcaaaga aagttaataa gtttgagatt atggatcaag
     7201 ttgatgatga tagacttgca caacttattc ctgattttta tgtctttcca gaaaaaagtg
     7261 taagctataa tatactaaag caaggtaagc atgcttttat tttgagcatt ggtaacagag
     7321 caataatgca ttgtgcaagg taaactaaaa tagataacaa aaagatggaa cctcgttaat
     7381 cataaaacaa taaaattaaa tattctcgca gtatatggca gtctaaagca tcaaagtttt
     7441 gatcaacatc tttcatttta gacatctcct tgcaatgtaa aatatcatgt atcaacaaca
     7501 tctggtgcaa atccatgagt cgaactcgac attcatctta gctcgattat tattccttcg
     7561 tacagtcgat gtaaacaata cagaaagagg attattaaga accagtttac tcagtgaatc
     7621 aatagaggaa aggactaacg tttctttaaa agaattgatt tcatcatctg ttaaactaaa
     7681 ctcatcattg acagatcgtg agatataact gcttttaact ttactcttta cgttgacttt
     7741 attgacagaa ttaacattca caaatcttga atttaatgcc catgatgttg tttgcgtgag
     7801 aaccttctca gcattaagga atttttttaa cggaatcctt ggtttctttt tcgagtccga
     7861 atttacaata tcatgcatat agaccatttc atgaatctgc gaaaactcaa tgtccattat
     7921 atacctcaca tttttaccgt gactcgatgt tactgttcaa taatcacctt ccatcaatac
     7981 taaaattaat acccctaatg tgccgataac aaatatagtc attctacgta atgtctccat
     8041 aaggtgatat ttgacattat cagaagctgc gaattcggat tttgctctaa tcaaatgaga
     8101 gaaatgtaaa atgaagccac gaaatattaa taatagccta ccactgcaac cattagttcc
     8161 tgatcaggag aacaaaaata agaaaaatga agagaaatcc gttaatccag ttaaaatcac
     8221 aatggggtct ggtttaaatt atattgaaca agaatctctt ggaggaaaat atctaacaca
     8281 tgatttgtca ataaagatag cggatatttc tgaagagata attcagcaag caatattatc
     8341 tgctatgagc atatataaat tttcgataac agatgattta atgagtatgg ctgtaaatga
     8401 actcataaaa ctgaccaaaa tagagaataa tgtagacctg aataaattca ctactatatg
     8461 cacagacgtt ctatcccccc gcgtcaccag acataataaa gaaaaaaaac aaacgacatt
     8521 ctacccttct caaaaatccc ctttttaatt ttcattgaaa aaatggcaat tgatactatg
     8581 aatccaggtc atacacatta aggcatttat gtaatgt
//
LOCUS       DAZGLJ010000184         8616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000184 DAZGLJ010000000
VERSION     DAZGLJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.184"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(76..696)
                     /gene="entD"
                     /locus_tag="ACHK49_003806"
     CDS             complement(76..696)
                     /gene="entD"
                     /locus_tag="ACHK49_003806"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO9264373.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(733..831)
                     /locus_tag="ACHK49_003807"
                     /pseudo
     CDS             complement(733..831)
                     /locus_tag="ACHK49_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(871..3111)
                     /gene="fepA"
                     /locus_tag="ACHK49_003808"
     CDS             complement(871..3111)
                     /gene="fepA"
                     /locus_tag="ACHK49_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO9264374.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDILVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGMSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            3354..4555
                     /gene="fes"
                     /locus_tag="ACHK49_003809"
                     /pseudo
     CDS             3354..4555
                     /gene="fes"
                     /locus_tag="ACHK49_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
     gene            4558..4776
                     /gene="ybdZ"
                     /locus_tag="ACHK49_003810"
     CDS             4558..4776
                     /gene="ybdZ"
                     /locus_tag="ACHK49_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO9264375.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            4773..>8616
                     /gene="entF"
                     /locus_tag="ACHK49_003811"
     CDS             4773..>8616
                     /gene="entF"
                     /locus_tag="ACHK49_003811"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO9264376.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPVDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFV"
BASE COUNT         1842 a         2319 c         2392 g         2063 t
ORIGIN      
        1 gtggtaaggc acctgcttta cactttcgcc cgcggtcagt gatggctgcg ggcgaatcgt
       61 accagatgtt gtcaattaat cgtgttggca cagagttatg actatttttt cttttatctg
      121 ccagtgcaca gcaaacatct cattctcacg atgaatgatg acctgctgtt tattccagct
      181 aattatctga tagtccagaa aacctgcatc agtttggatc tcacttgcct taaatgcgct
      241 ctctttggcg gaaaatgcca gtgtcagcgc cagagaaaag gctaaaccgc agtctgcgag
      301 tcgctcgtgt tctgctggtg taataatgtt gtctgtcaat tcggtcgctg tttgcgcaga
      361 aaaaatttct tcgatatcaa cgccaatcgg ttgacgggaa accacggcca atgccgtagc
      421 cccgcagtgg ctaatactgc cgtatacctc cgcaggccac accggttggc gtagctcgcc
      481 gattgcgggc acacatttat agccatattc ccgcagcgca taaattgcag cgatccgccc
      541 ggctaaatgt tcggctttgc gcttccgtcc agcgtgttgc agttgcgcgt aatgcggcag
      601 ccagagtaaa tcctgctcac aaaaattcgc cggatcgaac tcaacaaaat gcagcgtatg
      661 tccggcaaag gggagggagg tatgcgtagt tttcatgtcg accatattag agactgatga
      721 caaacgctaa actgcctgat gcgctacgct tatcaggcct acatggtcct gcaatatatt
      781 gaattggcaa gatttttgta ggccggataa ggcgttcacg ccgcatccgg catgaacgac
      841 gcgcactttg tcaacaatct gacgttagca tcagaagtga gtgtttacgc tcatgtacca
      901 cgtacgtccc ggctcgttat aggtatacgc cccggcaccc gcgatatagt tggcccctgc
      961 cagatcgccc gtggtctggg cattacccgc acgccacaaa cgtttgtcga acaggttgtc
     1021 cacgccgccg gtcagactga cattcttcgt cacatcccag gtcgcgctca gacccacaat
     1081 gctgtaagga ctgatttctt tggtttccgg gccaaccgct ggctgacctt tgtagttgta
     1141 ttttttcggc tgctgcttac cgtaccaggt gaaggtcgtt tgcatcgaca aatcttcccg
     1201 cgcctgccag ctcagcgttg agttcaacgt gtactccggg atgatcgaca aacggtcgcc
     1261 cgtggttttg ttttcactct tcagcatata agtgatgtta ttggtccaca tcaccgtttc
     1321 gctaactggt acgtttaacg atccttccag accttcaacc actgctttcg gcacgttatc
     1381 ccactgatag agatcggtgc cgactgcgtt ttgccctaca gccacatagc ccgcttcaat
     1441 cttattgcga taatcgttac ggaaccaggt gacgcccgcc agccagccgt cgcgtttgaa
     1501 ctccagacca atctctttgt tgatgctggt ttctgctttc aggtcatcgt taccttgcag
     1561 atagcagccg cccgcgctgg catagcagcc ctgaccttta ctgtagagaa tgtagttcgg
     1621 gttagtctgg tacaggctcg gtgctttata agcacgggca atgcccattt tgagcgtgaa
     1681 gtcatcgcct aaaccttgcg atatgttcag cgccgggctc cagttattgc cgacaatact
     1741 gtgatgatcg aaacgcagcc ccggcgttac gatggtgctg tcagtcagct ccatgttgtt
     1801 ttcggcaaac agcgagaaaa tttctgcttt tgaatacggg ctacggtcgg tggcactcat
     1861 accatcaata gcgccgccgg tgttcgcccc ggtcagcgcc tgggtattgg aactcaagtc
     1921 cttcatccgt tgctgattcc actccgtacc cagcgtcagc gtctggttaa cgaggatatc
     1981 aatcggcagg ttaacttcgc tgtgcagcat cacgtcatcg agatcgatat cgacgaaatc
     2041 ctgtgtcgct ttttcgttaa atttcccttc ggtaccgccc gccagacctt ccggaatacg
     2101 cgagttacgg gtgtgttcat actgcaccca gttgctggtg gtcacgccgt tatcccagcc
     2161 accgttccag gtcagcgcgt agttctggcg atacaggcgg ttggtttcat cgccatattt
     2221 cgagcgagtg taagcgtcag agttggtgtt ctgggtatcg cccgcataca ggttaccctg
     2281 gcggctgtaa cccgcttcca gttccagcga ttgcaatggt gcgaagtccc agcgcaccac
     2341 gccgttaata tctttattga tcaccccttc gcgcccggct ggtaacgtcg tggcatacgt
     2401 tccggcacgc gcggactgat ggccctggtt gatatcccac gcgtcagcct gggttttgtc
     2461 gaggttgcca tacaagcgga agctgaattc gtcgcccagc ggaccggtca ggctgaagtt
     2521 ggtgcgttta gtggctcctt cctctttatg ttctggtgca ttgaaataag cgtcccagga
     2581 gccgtgccac tcaccgctgc cttttttggt aatgatgtta accacgccgc ccgccgcgcc
     2641 gttgccataa cgcgcagctg ccggaccacg cagaacttca atacgttcaa tcatttcagg
     2701 tggcacccag gaggtatcac cacgggtatc gcgctcgcca cgccagccct gacgcaccga
     2761 gttacggctg cttaccggct tgccgtcaat caaaatcagc gtgttttccg gacccatacc
     2821 gcgaatatca atctgtcggt tattcccacg ctgaccactg gtggagttac cggtcaggtt
     2881 aacgcctggc atggtacgga tgatctccga cacatcgcgg gcaacggggt ttttgcggat
     2941 ttcatctgcg gtgatggtcg aaacgccagg cgcctgtaag ttctgctcgg cggcggtaac
     3001 gacaatggta tcgtcatgtg aaacaggagt atcggtcggc tcttgtgcct gcgctacccc
     3061 ataaatcccc agattgacca acaaggccag ggaatgaatc ttcttgttca ttgttttatt
     3121 cctgcatttt tgccacgaat tgcaactgtc gggcatggtc gtcctcaaca cgacgcatcc
     3181 cgctaccgcg aaaacctttg atcctgaaag acacgcagtg cagttggtta ataaatgtcc
     3241 gcgcttccca cggcgcgcca ttacgctatt gcaaatgcaa atagttatca ataatattat
     3301 caatatattt ctgcaatcaa tgaaaaattg cacagtaaac atggggttat ggtgtgacgg
     3361 cgttaaaagt aggaagtgag agctggtggc agtcgaaaca tggcccggaa tggcagcgtc
     3421 tgaatgacga aatgtttgag gtcactttct ggtggcgtga tccccaaggt tctgaagaat
     3481 actcgacgat aaagcgcgta tggatctaca tcactggtgt gaccgatcat catcagaaca
     3541 gccagcccca gtcgatgcag cgaattgcag gtactgatgt ctggcagtgg acgacacaac
     3601 tcaatgccaa ctggcgcggc agctactgct ttattcccac cgaacgcgat gacatttttt
     3661 ctgcaccatc ccccgatcgc ctcgaattac gtgaaggctg gcgaaaacta ttaccccagg
     3721 caatagccga tccgctgaat ccacaaagct ggaaaggcgg gcgagggcac gctgtttctg
     3781 cactcgaaat gccgcaagcg cctctgcaac cgggatggga ttgtccgcaa gcgccagaaa
     3841 tagcagccaa agaaattatc tggaaaagtg aacggttgaa aaattcacgt cgtgtatgga
     3901 tttttaccac cggcgatgca acagcagaag aacgtccgct ggcagttttg ctcgatggcg
     3961 aattttgggc gcaaagtatg cccgtctggc ctgtgctgac ttcgctgacc catcgtcagc
     4021 aacttcctcc cgccgtgtat gtgttgatcg acgctatcga caccacgcac cgcgcccacg
     4081 aactgccgtg taatgcggat ttctggctgg cagtacagca agagttatta cccctggtga
     4141 aagctattgc cccttttagc gatcgcgccg atcgcaccgt ggtcgccggg cagagttttg
     4201 gtgggctgtc cgcgctgtat gccggactgc actggcctga acgctttggc tgtgtattaa
     4261 gccagtcagg atcgtactgg tggccgcatc ggggcgggca gcaagagggc gtgttacttg
     4321 aaaagcttaa agctggtgaa gttagcgccg aaggtctgcg cattgtgctg gaagcgggta
     4381 ttcgcgagcc gatgattatg cgggccaatc aggcgctgta tgcgcaatta caccccataa
     4441 aagaatccat tttctggcgt caggttgacg gcggacatga tgcgctttgt tggcgcggtg
     4501 gcttgatgca ggggctaatc gacctctggc aaccactttc catgacagga gttgaatatg
     4561 gcattcagta atcccttcga tgatccacag ggagcgtttt acattttgcg caatgcgcag
     4621 gggcaattca gtctgtggcc gcaacaatgc gccttaccgg caggctggga cattgtgtgt
     4681 cagccgcagt cacaggcgtc ctgccagcag tggctggaag tccactggcg tactctgaca
     4741 ccggccaatt tcacccagtt gcaggaggca caatgagcca gcatttacct ttggtcgccg
     4801 cacagcccgg catctggatg gcagaaaaac tgtcggattt accctccgcc tggagcgtgg
     4861 cgcattacgt tgagttaact ggagatgttg atgcgccatt actggcccgc gcggtggttg
     4921 tcggactagc gcaagcagat acgttgcgga tgcgttttac ggaagataac ggcgaagtct
     4981 ggcagtgggt cgatgatgcg ctgatattcg aactgccaga aattatcgac ctgcgaacca
     5041 atattgatcc gcacggtact gcgcaggcat taatgctggc ggatttgcaa caagatttgc
     5101 gcgtcgatag cggtaaacca ctggtctttc atcagctgat acatgttgcg gataaccgct
     5161 ggtactggta tcagcgttat caccatttgc tggtcgatgg cttcagtttc ccggccatta
     5221 ctcgccagat cgccaatatt tactgcacat ggctgcgtgg cgaaccaacg cctgcttcgc
     5281 cgtttacgcc tttcgctgat gtagtggaag agtaccagca ataccgcgaa agcgaagcct
     5341 ggcagcgtga tgcggcattc tgggcagaac agcgtcgtca actgccgccg cccgcgtcac
     5401 tttctccggc acctttagcg gggcgcagcg cttcggcaga tattctgcgc ctgaaactgg
     5461 aatttaccga cggggaattc cgccagctgg ctacgcaact ttcaggtgtg cagcgtaccg
     5521 atttagccct tgcgctggca gccttgtggc tggggcgatt gtgcaatcgc atggactatg
     5581 ccgccggatt tatctttatg cgtcgactgg gctcggcggc gctgacggct agcggacccg
     5641 tgctcaacgt tttaccgttg ggtattcaca ttgcggcgca agaaacgctg ccggaactgg
     5701 caacccgact ggcagcacaa ctgaaaaaaa tgcgtcgtca tcaacgttac gatgccgaac
     5761 aaattgtccg tgacagcggg cgagcggcag gagaggaacc gctgttcggt ccggtactca
     5821 atatcaaggt atttgattac caactggata ttcctggtgt tcagacgcaa acccataccc
     5881 tggcaaccgg tccggttaat gaccttgaac tggccctgtt cccggatgaa cacggtgatt
     5941 tgagtattga gatcctcgcc aataaacagc gttacgatga gccaacgtta atccagcatg
     6001 ctgaacgcct gaaaatgctg attgcccagt tcgccgcgga tccggcgctg ttgtgcggcg
     6061 atgtcgatat tatgctgcca ggtgagtatg cgcagctggc gcagatcaac gccactcagg
     6121 ttgagattcc agaaaccacg ctgagcgcgc tggtggcaga acaagcggca aaaacaccgg
     6181 atgctccggc gctggcagat gcgcgttacc tgttcagcta tcgggaaatg cgcgagcagg
     6241 tggtggcgct ggcgaatctg ctgcgtgagc gcggcgttaa accaggggac agcgtggcgg
     6301 tggcactacc gcgctcggtc tttttgaccc tggcactcca tgcaatagtt gaagctggcg
     6361 cggcctggct accgctggat accggctatc cggacgatcg cctgaaaatg atgctggaag
     6421 atgcgcgtcc gtcgctgtta attaccaccg acgatcaact gccgcgcttt agcgatgttc
     6481 ccaatttaac aagcctttgc tataacgccc cgcttacccc ccagggcagt gcgccgctgc
     6541 aactttcaca accgcaccac acggcttata tcatctttac ctctggctcc accggcaggc
     6601 cgaaaggggt aatggtcggg cagacggcta tcgttaaccg cctgttatgg atgcaaaatc
     6661 actacccgct tacaggcgaa gatgtcgttg cccaaaaaac gccgtgcagt tttgatgtct
     6721 cggtgtggga gtttttctgg ccgtttatcg caggggcaaa actggtgatg gctgaaccgg
     6781 aagcgcaccg tgacccgctc gctatgcagc aattctttgc cgaatatggc gtaacgacca
     6841 cgcactttgt gccgtcgatg ctggcggcat ttgttgcatc gctgacgccg caaaccgctc
     6901 gccagaattg cgcgacgttg aaacaggttt tctgtagtgg tgaggcctta ccggctgatt
     6961 tatgccgcga atggcaacag ttaaccggcg cgccgttgca taacctgtat ggcccgacgg
     7021 aagcggcggt agatgtcagc tggtatccgg cttttggcga ggaactggca caggtgcgcg
     7081 gcagcagtgt gccgattggt tatccggtat ggaatacggg tctgcgtatt cttgatgcga
     7141 tgatgcatcc ggtgccgccg ggtgtggcgg gtgatctcta tctcaccggc atccaactgg
     7201 cgcaggggta tcttggacga cccgatctga ccgccagccg ttttattgcc gatccttttg
     7261 ccccaggtga acggatgtat cgtaccggag acgtcgcccg ctggctggat aacggcgcgg
     7321 tggagtacct cgggcgcagt gacgatcagc taaaaattcg cgggcagcgt atcgaactgg
     7381 gcgaaatcga tcgcgtgatg caggcgctgc cggatgtcga acaggcagtt acccacgcct
     7441 gtgtgattaa ccaggcggca gccaccggtg gtgatgcgcg tcagttggtg ggctatctgg
     7501 tgtcgcaatc gggactgccg ttggatacca gcgcattaca ggcacagctt cgcgaaacat
     7561 tgccgccgca tatggtgccg gtcgttctgc tgcaacttcc gcagttgccg cttagcgcca
     7621 acggcaagct ggatcgcaaa gccttaccgt tgcctgaact gaagacacaa gcgtcgggac
     7681 gtgcaccgaa agcgggcagt gaaacgatta tcgccgcggc attcgcgtcg ttgctgggtt
     7741 gtgacgtgca ggatgccgat gctgatttct ttgcgctggg cggtcattcg ctactggcaa
     7801 tgaaactagc agcgcagtta agtcgacagt ttgctcgtca ggtgacgccg gggcaggtga
     7861 tggtcgcgtc aaccgtcgcc aaactggcaa cgattattga tggtgaagag gacagctccc
     7921 ggcgcatggg attcgaaacc attctgccgt tgcgtgaagg taatggcccg acgctgtttt
     7981 gtttccatcc ggcgtccggt tttgcctggc agtttagcgt gctctcgcgt tatctcgatc
     8041 cacaatggtc gattattggc attcagtcgc cgcgccctca tggacccatg cagacagcga
     8101 caaacctgga tgaagtctgc gaagcgcatc tggcaacgtt acttgagcaa caaccgcacg
     8161 gcccttatta cctgctgggg tattcccttg gcggtacgct ggcgcagggt attgcggcgc
     8221 gactgcgtgc ccgtggcgaa caggtggcat ttcttggttt gctggatacc tggccgccag
     8281 agacgcaaaa ctggcaggaa aaagaggcta atggtctgga cccggaagtg ctggcggaga
     8341 ttaaccgcga acgcgaggcc ttcctggcgg cacagcaggg aagtacttca acggagttgt
     8401 ttaccaccat tgaaggcaac tacgctgatg ctgtgcgctt gttaaccacc gcccatagcg
     8461 ttccggttga tggaaaagca acgttatttg ttgctgagcg cacacttcag gaaggtatga
     8521 gtcccgaacg tgcctggtcg ccgtggattg cggagctgga tatctatcgt caggattgtg
     8581 cgcatgtgga tattatctct ccaggggcgt ttgtaa
//
LOCUS       DAZGLJ010000185         8564 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000185 DAZGLJ010000000
VERSION     DAZGLJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.185"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..2640
                     /gene="cas3"
                     /locus_tag="ACHK49_003812"
     CDS             <1..2640
                     /gene="cas3"
                     /locus_tag="ACHK49_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFO9264377.1"
                     /translation="WGKTRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKRNCFGLADYF
                     RQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQEGARKNYEKISHSTL
                     GYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGQPPDRMDELDNFLPED
                     KAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYISATVVLADWTGSSTRFFP
                     RVAHPMDIKGYWQKTLIQAQNALTVFPLKAKVAPFNGINTLFPFIENPTPLQQKVLDL
                     DISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGLFFGLPTMATANAMYDRLV
                     KTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCAAWFAD
                     SNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCILEG
                     LIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQQEAPFLEKDDYPWLTHVTKS
                     DVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSVDDAIKVHRQ
                     LLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKYCSLQRASQVIVCTQVIEQSV
                     DIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAPVWDDS
                     PGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGEDVVMPE
                     GFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLSTRLAEESVSLWLAT
                     CIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRLWCIEQRQDPEMAN
                     VILVNDDESCGYSATEGLIGKVG"
     gene            2738..4300
                     /gene="casA"
                     /locus_tag="ACHK49_003813"
                     /gene_synonym="cse1"
     CDS             2738..4300
                     /gene="casA"
                     /locus_tag="ACHK49_003813"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO9264378.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKNHGRWDDIWQDGLEAEKLREALLSLEHAFQFGADSPSFM
                     QDFEALKGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWPNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRIPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLKKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            4297..4836
                     /gene="casB"
                     /locus_tag="ACHK49_003814"
                     /gene_synonym="cse2"
     CDS             4297..4836
                     /gene="casB"
                     /locus_tag="ACHK49_003814"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO9264379.1"
                     /translation="MSIVKEEHKATLRKWREELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSKGRFTRL
                     SAVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQNQ"
     gene            4848..5903
                     /gene="cas7e"
                     /locus_tag="ACHK49_003815"
     CDS             4848..5903
                     /gene="cas7e"
                     /locus_tag="ACHK49_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO9264380.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWSAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            5914..6660
                     /gene="cas5e"
                     /locus_tag="ACHK49_003816"
     CDS             5914..6660
                     /gene="cas5e"
                     /locus_tag="ACHK49_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO9264381.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            6642..7292
                     /gene="cas6e"
                     /locus_tag="ACHK49_003817"
     CDS             6642..7292
                     /gene="cas6e"
                     /locus_tag="ACHK49_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO9264382.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGPAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFVQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            7289..8209
                     /gene="cas1e"
                     /locus_tag="ACHK49_003818"
     CDS             7289..8209
                     /gene="cas1e"
                     /locus_tag="ACHK49_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO9264383.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            8209..8502
                     /gene="cas2e"
                     /locus_tag="ACHK49_003819"
     CDS             8209..8502
                     /gene="cas2e"
                     /locus_tag="ACHK49_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO9264384.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
BASE COUNT         2111 a         2026 c         2376 g         2051 t
ORIGIN      
        1 tggggaaaaa cccgacgcgg cgagaaagac gggggcgacg actatcacct tctttgctgg
       61 cattcgctgg atgtagccgc gatgggctat ttaatggtta aaagaaattg cttcgggctg
      121 gctgattatt ttcgtcaatt agggatttct gacaaggaac aggcggctca atttttcgcc
      181 tggttgctgt gctggcacga tattggaaaa tttgcccgct cttttcaaca actttacctg
      241 ccccctgaac tcaagattca ggaaggtgcc agaaaaaatt acgaaaaaat ctctcattca
      301 acgctgggct actggttgtg gaatcattat ttaagtgaat gtcaggagtt acttccttca
      361 tcttcgctct ctccgcgcaa acttaggcga gtgatagaaa tgtggatgcc cgtgacgaca
      421 gggcatcatg gtcagccccc tgatcgtatg gatgaattgg ataattttct gcctgaagac
      481 aaagctgcag cacgagattt tctccttgaa atcaaaccac tgtttccgct catagagatc
      541 cccgcattct gggatgatga tgagggcatt gaacttataa aacacctttc ctggtatatc
      601 tctgcaaccg tcgtgctcgc agactggacg ggttcgtcaa cgcgattttt tccacgcgtc
      661 gcacatccaa tggatattaa aggttactgg cagaaaactt taattcaggc tcaaaacgcc
      721 ttaaccgtct ttcctctaaa agcaaaagtc gcgcctttca acggaattaa tacgctgttt
      781 ccttttattg agaatccgac accgttacag caaaaggtgc tggatctgga tatcagccag
      841 caaggaccgc agttatttat tctggaagat gtgactggcg caggtaaaac agaagcggcg
      901 cttatcctgg cgcacaggtt gatagctgcg gggaaagctc aggggctgtt ttttggtctg
      961 ccaacaatgg caacggccaa tgccatgtac gatcggctgg tcaaaacctg gcttgctttc
     1021 tattcgccag aatcccgccc cagcctggtg ctggcacata gtgcccgcac attaatggac
     1081 cgctttaatg aatcactctg gtctggtgat ttagtcgggt cagaagaacc ggatgaacaa
     1141 acgttcagtc agggatgtgc ggcctggttt gccgacagta acaagaaggc gttgctggct
     1201 gaaattggcg tcggcacgct ggatcaggcg atgatggcgg tgatgccgtt caaacataat
     1261 aatctgcggc ttctggggct gagtaacaaa atcctgctgg ctgatgagat ccacgcctgt
     1321 gacgcttaca tgtcgtgcat tcttgaaggg ttgatcgagc ggcaggcgcg tggcggaaac
     1381 agcgttattt tgctttctgc cacgttatct caacagcagc gcgacaaact cgtcgctgcc
     1441 tttgcgcggg gtacagaggg ccagcaagaa gctccgttcc tggaaaagga tgattacccc
     1501 tggctgacgc atgtcacgaa atccgatgtg aactcacacc gggtagcgac gcgcaaagat
     1561 gttgagcgta gcgtcagcgt gggttggctt catagtgaac aagagtgtat tgcgcgtatc
     1621 gaatcggcgg taagtcaggg gaaatgcatc gcctggatcc ggaattctgt cgatgacgct
     1681 attaaggttc atcgtcagct gcttgcccgc ggcgtcattc ctgcttccag cctttcactt
     1741 tttcatagcc gctttgcttt tagcgatcgc cagcgaattg aaacggaaac gctggcacgc
     1801 tttggtaaat attgtagttt acagagagcg tcgcaagtca ttgtttgtac acaagtaatt
     1861 gagcaaagcg tggatattga cctggacgaa atgatctccg acctggcccc tgttgatttg
     1921 ctgattcagc gagcaggacg attacagcgg catatccgcg atattaatgg tcagttaaag
     1981 cgtgacggaa aggacgagcg ttccccccct gaattgctga ttctggctcc cgtctgggac
     2041 gactctcctg gcgacgaatg gtttggcagt gccatgcgta acagtgcatt tgtctatccc
     2101 gatcatgggc gaatctggct gacgcagcgt gtgctgcgtg agcaaggtgc tattcagatg
     2161 ccacacgcag cccggcttct tattgaatcc gtctacggtg aggacgtggt aatgccggaa
     2221 ggatttgccc gcagcgagca ggagcaagtg ggcaaatatt actgcgatcg cgcaatggct
     2281 aaaaagtttg tgctgaactt caggcccggc tatgccgcca atatcaacga ttacctcccg
     2341 gaaaaactgt cgacacgtct ggctgaggag tctgtttccc tgtggctggc tacctgtatt
     2401 gacggtgtgg tgaagcctta tgccaccggt gctcacgcat gggaaatgag cgttgtcaga
     2461 gtgcgccgaa gctggtggaa aaaacatcgg gatgagtttt ctttactgga gggagaggcg
     2521 ttcaggctgt ggtgcattga acagcgacaa gatccggaga tggcaaacgt gattttagtc
     2581 aacgatgacg aaagttgcgg gtattcggct acggaaggat tgattggcaa ggttggttga
     2641 gatgtctgcg acaggactct ctgaatagtt gatacaccga aaaggatgga aagatggaca
     2701 accgacctgg gatggtcttt tttacgagag gatgcgtatg aactcgtttt cacttctgac
     2761 aaccccgtgg ttgcctgttc gttttaaaga cggaacgaca ggcaagctgg caccagtcga
     2821 tctggctgat gaaaatgttg tcgatatcgc tgcgccgcgg gcagatctcc agggggcggc
     2881 ctggcagttt ttgctggggc tactacaaac cagcttcgcg cccaaaaatc acggtcgttg
     2941 ggatgatatc tggcaagacg gactggaggc tgaaaagcta cgggaagcat tgctgtcctt
     3001 agaacacgct ttccagtttg gggcagattc cccttcattt atgcaggatt tcgaggcgct
     3061 caagggagat aaagttcagg tcgcttcgct actgcctgag attcccggcg ctcaaacaac
     3121 gaagtttaat aaagaccact ttattaagcg tggcgtgact gaacacgtat gccctcattg
     3181 ttccgcgtta gctctgttct ccctacagtt aaatgcgcca tcaggcggca aaggctatcg
     3241 caccggtttg cgcggcggtg ggccgatgac cactctgatt gaattacagg aatatcaagg
     3301 caatcaacaa acgcccttgt ggcgcaaact gtggcccaat gtgatgccgc aggatgaagc
     3361 cgacttaccg ctacccaaaa aatttgacga tctggttttc ccctggcttg gcccgacgcg
     3421 aaccagcgaa ctggccggtg cggtggtaac ccatgatcag gtcaataaac tccaggctta
     3481 ctggggaatg ccgcgccgta ttcgtattga ttttaatacc acgacagtcg gcaactgcga
     3541 tatttgcggt gaacagagcg acgcgctcct gagcctgatg acgaccaaaa attacggtgc
     3601 gaattatgcc atgtggcagc atcccttaac gccttaccgt ataccactta aagagggtgg
     3661 cgagttttac tcggtcaaac cacaaccggg cggtttaatc tggcgcgact ggttaggcct
     3721 tatcgaaacg ggtaaatcag aaaacaatac ggaacttccc gcgctggtgg tgaaactctt
     3781 taatgccagc agtctgaaac aggcaaaagt aggcctgtgg ggatttggct atgatttcga
     3841 caacatgaaa gcgcgctgtt ggtacgaaca ccatttcccg ctgctgctca agaaaaaaga
     3901 aggccagata ccgaaactgc gtctggctgc gcaaacggct tcacggattc taagtctgtt
     3961 acgaagtgca ttgaaagaag cgtggttctc cgatccaaaa ggtgcacggg gtgatttcag
     4021 ttttgtggat atcgacttct ggaacaaaac tcagcatcgc ttcctgaggt tagtgcgcca
     4081 aattgaagaa ggtcaggatg cggacgaatt actcggcaaa tggcaaaagg aaatttggtt
     4141 attcgcgcgt caggattttg acgagcgtgt attcaccaat ccatatgagc ctgttgattt
     4201 gaaacgcgtt atgaccgcgc gcaagaaata tttcacaaca tcggcggaga agcaaagtgc
     4261 taaagccgcc agggagaaaa agcaggaggc tgctgaatga gtattgtaaa agaggagcac
     4321 aaagccacgc tgcgtaaatg gcgcgaagag ttgcaggcta aacgcggcaa tcgtgcaagt
     4381 ctgcggcgta gcacaacggt taatgatgtt tgcttgtcag aagggtttcg ttctctgctg
     4441 atgcagaccc acactttgtg gaaaattgaa gcacaagagt ggcgttttac cgcactggcg
     4501 ctggtcgccg cagttagcgc caatgtaaaa gctattgatg aacggcagcc ttttgccgcg
     4561 cagctggcgg cggtaatgtc aaaggggcgt tttactcgtc tgtcggcagt aaaaacaccg
     4621 gatgaattac tgcgccagtt acgccgcgca gtcagattat taaacggttc cgtcaatctt
     4681 gactctctgg cagagggggt cttccgttgg tgccaggaga gcgacgacct gctcaatcat
     4741 caccgccgcc agcagcggcc aaccgaattt atccgtatcc gctgggcgct ggaatattac
     4801 caggctggcg acgccgataa cgaacaaaat caataaagga ctatctcatg acaacattta
     4861 ttcagcttca tttgttaacc gcttaccctg ctgccaacct taaccgtgat gataccggtg
     4921 cgccaaaaac tgtggtgctg ggtggagcaa cgcgtctgcg cgtttcctcg caaagtctga
     4981 aacgtgcgtg gcgcacttct gcactttttg aacaggcact ggcgggccat attggtattc
     5041 gcagtgggcg tattgcgcgt gaggcggcaa ctatcctgat tgagaaaggg attgaagata
     5101 aaaaagccat cgaatggtcg gcaaaaattg ctgattatct tgggaaagct aaaaatgaca
     5161 aaaaaccaaa agatccgctc actaacgccg aaactgaaca gttagtccat atcagcccgg
     5221 cagaatttga cgccgtaaaa gcgctggccc atcagcttgc agaagaaaag cgcgcgccaa
     5281 aagaggaaga tctcgcgttg ttacgcaaag accgtatggc ggtagatatt gccatgtttg
     5341 gtcgtatgct ggcgaataaa cccgagttta atgttgaagc cgcctgccag gtagcgcacg
     5401 catttggtgt cagtgaaacg attgtcgaag atgatttttt caccgccgtt gatgatttgc
     5461 gccaggcttc tgaagatgcc ggtgccgggc atcttggtga aaccggattc ggttctgcac
     5521 tgttctacac ctatatctgc attgataaag atctgctggt cgaaaacctt ggcggtgacg
     5581 aagcgttagc taatcagacc atacgtgcct ttacggaagc cgcacttaaa gtctccccaa
     5641 ccggcaaaca gaacagcttt gccagccgtg cctacgcctc ctgggcgctg gcagaaaaag
     5701 gcaccgatca accacgctct ctggcggcgg ctttctatga acccattaat ggcacacgtc
     5761 agttagatgt ggcggtgcag cgtattacaa cgcttcgcga aaacatgaat acggtctacg
     5821 aacagaagac cgaatgcgca agttttgacg tgatgaacaa acagggaagc atgaaggacg
     5881 tgctggactt tatctgcgcg taaggggcgg cttatgagcc aatatctgat ttttcagctt
     5941 catgggccga tggcgtcctg gggggtcgat gctccaggtg aagttcgtca tacccatgaa
     6001 cttccttcgc gctcagcatt gctggggcta ctggctgccg gggtagggat tcggcgtgat
     6061 gataccgaac gcttaaacgc gtttaatcgc cactattcac tggtggtttg cgccagccgt
     6121 aacccgcgct gggcacggga ttatcacacg gtccagatgc caaaagaggt gcgtaaagca
     6181 cgttatttca gccgtcgcga agagttgagc gaccctgagc ttttgagcgc gattatctcc
     6241 cggcgcgact actacaccga tgcctggtgg atggtggcgg tggcaacaac agccgatgcg
     6301 ccttacagcc tcgaacagtt gcaggacggt ttacgccatc cagtttttcc gctttatctg
     6361 gggcgaaaaa gtcatccgct ggcattacct cttgcgccgt tactgctcga aggcaacgcg
     6421 tgtgatgcct tatgtaatgc ataccaacag tatcaggatc atttccataa actgaaagtc
     6481 tcactcccga aacttcagga tgaatgctgg tgggaaggga aacacgacgg cctggtcgcg
     6541 agcaaaatat tacgccgccg ggatgttcct ttaaatcgtc agcagtggct gtttggggaa
     6601 cgcaccgtca atcaggggcc gtggctcagc aaggaggaac catgtacctc tcaagaataa
     6661 ccttacatac cggccagctt tcgcctgcgc agttgctgca tctggtggat cgcggtgaat
     6721 atgtgatgca tcagtggctg tgggatctct tccctggcgg caaagaaagg caatttcttt
     6781 atcgtcggga agaactccag ggcgcgtttc gcttttttgt actgtcgcag gaacgcccgg
     6841 cggaaagtga tacgttcacc atagaatgcc ggtcatttgc gcccgagctg tgtaccggac
     6901 aacaactttg tttcaacctg cgggcgaatc caacaatctg taaagcgggc aagcgccacg
     6961 atctgctgat ggaggcgaaa cggcaggtga gggggccggc ggaaggaagt gatgtctggt
     7021 tgcatcaaca acaggcggcg ctggactggc tggcagcgca gggggagaga agcggtttta
     7081 cactgctgga tacctcggtt gatgcctacc gacaacagca actgcggcgg gaaaactccc
     7141 ggcaactgat ccagttcagt agcgtggatt atacggggat gcttacggtc accgatcctg
     7201 ggttatttgt gcaacgcctg agccaggggt atggtaaaag ccgggcgttc ggctgtggtc
     7261 tgatgctgat caaacccgga gcagaggcgt gacgtttgta ccactgagcc cgatcccatt
     7321 aaaagatcgc acctccatga tcttcctcca gtacggtcag atcgacgtac tggacggcgc
     7381 tttcgtgctg atcgacaaaa cagggatccg aacgcatatt ccggtgggat cggtcgcctg
     7441 cattatgctc gaaccaggaa cgagagtttc ccacgcggcg gtgcatctgg ccgccacggt
     7501 gggaacactg ctggtctggg tcggtgaagc gggcgttcgc gtttactcct ccggacaacc
     7561 cggtggggcg cgggcagata aattactcta ccaggcaaag ctggctttaa cggaagatct
     7621 acggttgaag gtggtgcgca aaatgtatga attacgtttt cgtgaaccac agccagctcg
     7681 ccgttcagtg gagcagttac gtggaattga gggatctcgc gttcgccaga cgtatgcatt
     7741 actggcgaaa caatatggtg tgaaatggaa tggtcgcaaa tacgatccta aagactggga
     7801 aaaaggcgat gttgtgaatc gctgcatcag tgctgctaca tcatgtctgt acggtatatc
     7861 tgaagcggca gtattagccg cgggatatgc gcccgctatt gggtttatcc atagtggcaa
     7921 accgctttca tttgtttatg acatagccga tatcattaaa tttgattcgg ttgtgccaaa
     7981 ggcatttgaa atagcagcga ggcaacccgc agaacctgat aaagaagtca gattagcctg
     8041 tcgcgatatt ttccgtagca ctaagttaac gggcaaatta ataccgttaa ttgaggaagt
     8101 ccttgccgca ggtgaaattg aaccgccaca acctgcgccg gatatgttac cgccagccat
     8161 ccccgaacct gaaacgttgg gcgatagcgg tcatcgagga cgcggctgat gagcatggtc
     8221 gtggttgtta cagaaaatgt cccgccgcgc ttacgtggac ggctcgcaat ctggctactg
     8281 gaagtgcgtg ccggtgtgta tgttggtgat acatcaaaac gtattcggga gatgatctgg
     8341 cagcaaatta cccaactggc tggttgcgga aatgtggtga tggcctgggc gaccaatacc
     8401 gagtcgggtt ttgaatttca gacctgggga gaaaacagac gtattccggt ggatttggat
     8461 gggttacgtt tggtttcttt tcttcctgtt gataatcaat aagttatccg ttctttaaaa
     8521 ataaggaaat gttttaattt agttggtaga ttgttgatgc ggaa
//
LOCUS       DAZGLJ010000186         8562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000186 DAZGLJ010000000
VERSION     DAZGLJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.186"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(355..1335)
                     /locus_tag="ACHK49_003820"
                     /pseudo
     CDS             complement(355..1335)
                     /locus_tag="ACHK49_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004019902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1632..2234
                     /locus_tag="ACHK49_003821"
     CDS             1632..2234
                     /locus_tag="ACHK49_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFO9264385.1"
                     /translation="MKNDKAWIGDLLGGPLMSRESRVIAELLLTDPDEQTWQEQIVGH
                     NILQASSPNTAKRYAATIRLRLNTLDKSAWTLIAEGSERERQQLLFVALMLHSPVVKD
                     FLAEVVNDLRRQFKEKLPGNSWNEFVNSQVRLHPVLASYSDSSIAKMGNNLVKALAEA
                     GYVDTPRRRNLQAVYLLPETQAVLQRLGQQDLISILEGKR"
     gene            2231..2833
                     /locus_tag="ACHK49_003822"
     CDS             2231..2833
                     /locus_tag="ACHK49_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFO9264386.1"
                     /translation="MIDPVLEYRLSQIQSRINEDRFLKNNGSGNEIGFWIFDYPAQCE
                     LQVREHLKYLLRHLEKDHKFACLNVFQIIIDMLNERGLFERVCQQEVKVGTETLKKQL
                     AGPLNQKKIADFIAKKVDLAAQDFVILTGMGNAWPLVRGHELMSALQDVMGFTPLLMF
                     YPGTYSGYNLSPLTDTGSQNYYRAFRLVPDTGPAATLNPQ"
     gene            2845..6486
                     /gene="brxC"
                     /locus_tag="ACHK49_003823"
     CDS             2845..6486
                     /gene="brxC"
                     /locus_tag="ACHK49_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFO9264387.1"
                     /translation="MNIEQIFEKPLKRNINGVVKAEQTDDASAYIELDEYVITRELEN
                     HLRHFFESYVPATGPERIRMENKIGVWVSGFFGSGKSHFIKILSYLLSNRKVTHNGTE
                     RNAYSFFEDKIKDALFLADINKAVHYPTEVILFNIDSRANVDDKEDAILKVFLKVFNE
                     RIGYCADFPHIAHLERELDKRGQYETFKAAFADINGSRWEDERDAYYFISDDMAQALS
                     QATQQSLESSRQWVEQLDKNFPLDINNFCQWVKEWLDDNGKNILFMVDEVGQFIGKNT
                     QMMLKLQTITENLGVICGGRAWVIVTSQADINAAIGGMSSRDGQDFSKIQGRFSTRLQ
                     LSSSNTSEVIQKRLLVKTDEAKAALAKVWQEKADILRNQLAFDPTTTTALRPFTSEEE
                     FVDNYPFVPWHYQILQKVFESIRTKGAAGKQLAMGERSQLEAFQTAAQQISAQGLDSL
                     VPFWRFYAAIESFLEPAVSRTITQACQNGILDEFDGNLLKTLFLIRYVETLKSTLDNL
                     VTLSIDRIDADKVELRRRVEKSLNTLERLMLIARVEDKYVFLTNEEKEIENEIRNVDV
                     DFSAINKKLASIIFDDILKSRKYRYPANKQDFDISRFLNGHPLDGAVLNDLVVKILTP
                     KDPTYSFYNSDATCRPYTSEGDGCILIRLPEEGRTWSDIDLVVQTEKFLKDNAGQRPE
                     QATLLSEKARENSNREKLLRVQLESLLAEADVWAIGERLPKKSSTPSNIVDEACRYVI
                     ENTFGKLKMLRPFNGDISREIHALLTVENDTELDLGNLEESNPDAMREVETWINMNIE
                     YNKPVYLRDILNHFARRPYGWPEDEVKLLVARLACKGKFSFSQQNNNVERKQAWELFN
                     NSRRHSELRLHKVRRHDEAQVRKAAQTMADIAQQPFNEREEPALVEHIRQVFEEWKQE
                     LNVFRAKAEGGNNPGKNEIESGLRLLNAILNEKEDFALIEKVSSLKDELLDFSEDRED
                     LVDFYRKQFATWQKLGAALNGSFKSNRSALEKDAAAVKALGELESIWQMPEPYKHLNR
                     ITPLIEQVQNVNHQLVEQHRQHALERIDARIEESRQRLLEAHATSELQNSVLLPMQKA
                     RKRAEVSQSIPEILAEQQETKALQMDADKKINLWIDELCKKQEAQLRAANEAKRAADS
                     EQTYVVVEKTVIQPVPKKTHLVNVASEMRNAAGGEVLETTEQVEKALDTLRTTLLAAI
                     KAGDRIRLQ"
     gene            6532..>8562
                     /gene="pglX"
                     /locus_tag="ACHK49_003824"
     CDS             6532..>8562
                     /gene="pglX"
                     /locus_tag="ACHK49_003824"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFO9264388.1"
                     /translation="MNTNNIKKYAPQARNDFRDAVIQKLTTLGIAADKKGNLQIAEAE
                     TIGETVRYGQFDYPLSTLPRRERLVKRAREQGFEVLVEHCAYTWFNRLCAIRYMELHG
                     YLEHGFRMLSHPETPTAFEVLDHVPEVAEALLPENKAQLVEMKLSGNQDEALYRELLL
                     GQCHALHHVMPFLFEAVDDEAELLLPDNLTRTDSILRGLVDNIPEEDWEQVEVIGWLY
                     QFYISEKKDAVIGKVVKSEDIPAATQLFTPNWIVQYLVQNSVGRQWLQTYPDSPLKDK
                     MEYYIEPAEQTPEVQAQLAAITPASIEPESIKVLDPACGSGHILIEAYNVLKNIYEER
                     GYRGRDIPQLILENNIFGLDIDDRAAQLSGFALLMMARQDDRRIFTRDVRLNIVSLQE
                     SLHLDIAKLWQQLNFHQQVQTGSMGDMFAENTALTQTNSAEYQLLMRTLKRFVNAKTL
                     GSLIQVPQEEEAELKVFLDALYRLEQEGDFQQKSAAKAFIPFIQQAWILAQRYDAVVA
                     NPPYMGSKYQSLSVKKHLKDNFTGYDKDLFSAFIVRNLELSKQGAQLGFMTPFVWMFI
                     TTYEELRKKLIEQEIITSLVQLEYSGFDGATVPICTFTLQKGHINHFTGSYIRLSDFR
                     GASNQGPKTLEAIQHHNCGWFYEAQPDNFKKIPGTPIAYWLGQNINIFDNDKI"
BASE COUNT         2400 a         1986 c         1990 g         2186 t
ORIGIN      
        1 gcagatgtaa agaataccgc tttgttaatc tgacttgtag acttctttgg gattgtatca
       61 ctttcactca taatcattta ttccattaat taaattcgca ctggatagaa ggtactacct
      121 ctcacagagt agcaaagttc aactggctat gcggcgttaa tcggaatgca tggtggaatg
      181 tgaatcgcca ccggtttttt agggggttgt agagtggcat cggaaagagg tttttagggg
      241 gggaggttga taataccgcg cccccataat cttgccatct cttacattga gatgttaaaa
      301 gctacaaatg ctctccctat ttttcaatgt agacatttat agcaatataa aaatttattg
      361 ttcagctttt tcaacaacaa aaccattttg ccaaacaaac acaatcaaga gttcaatatt
      421 aacaataccc cattgattag aaatggcgac aatttcacca gaaggaagtg taatcaatgc
      481 atcttcgttg atatgatagc gactcttttg cttctctggt atctgatcga taagtataac
      541 cgtacgtttt ttaaagtctt cacttaatcc atttattaaa tcattataac ttgcagggtt
      601 aaattgtttt atacaatcgc gtaataactc aagcgctaac ttacgtttat taagtttttt
      661 atctttgcat atataccacg tatagccctt ctcttttttc tgactgctaa ttagctgctc
      721 atcacgtttt tcacgaaact taacttgata ttcttccaat tcaggaaccg gtatgatttg
      781 ctctgcattg atcagaacat catcattaaa tctataaggc gtcagacgaa cacatcgaat
      841 gtctacccct ttatctctta accaaatcgc agtggtagtc agttccttgc taaaatccga
      901 tgaagccaaa acgattctga tatctttacc aaaatcaaca agctcagttt catccagctc
      961 aacaaattca agtatttttt ctttcgcatt ttttctttcg catcggcatc gcggttttgt
     1021 tttttcaaat acgtctgaaa atactcacat gctttcgcaa aactcattgt gtaaatcatt
     1081 gccgcatatc gtagtgcctg taactccata tgcgtccccg tttcatcacg ttttaattcg
     1141 ataaccacta tatttgcctg tttatcgatt gccaataaat caattcgccg atggctatct
     1201 tcccagtcag aaatcaccag acaatcagga gcaataatat ctatattttt tgattgcttc
     1261 ctgtaatcct tccagagtaa aggtcgtttt ttctaaagca tgtggttctt tatttgcgat
     1321 attgtacagc accacgttca tcttcctttt ttaactgatt ttacagagac tttaatacag
     1381 ttaaaatttt atttcctgag ctgtaatcga ttaagttgat gcatttaatg ggaatgatat
     1441 agggtcattt ccagtctcac ttatagaaat ggctaaagca tgactctcgc caaaaccgtt
     1501 tatgtgttgt acataacgcg atcatccctc tcacaaattg ccttttctca tggcatctcg
     1561 cccggtcccc cattacaatc actttttgtt ttttgcgagc tgcattccag tcttcagagg
     1621 gtttttcgat gattaaaaat gacaaggcat ggataggaga cttgctgggc ggaccgctca
     1681 tgagcaggga aagccgcgtc attgccgaac tgttgctaac cgatcccgat gaacagacat
     1741 ggcaagagca aattgttggc cacaacattt tacaagcctc ttctcctaac accgcaaaac
     1801 gttacgcggc aacaatcagg cttcgcctga acacgctgga taaaagcgcg tggacattga
     1861 ttgccgaagg tagtgaacgg gaacgccaac aacttctgtt tgtggctctg atgctacatt
     1921 cgccggtagt taaggatttt ctggctgaag tggtgaacga tctgcgcagg cagttcaagg
     1981 aaaagttgcc tggcaatagc tggaacgaat ttgtgaatag ccaggttcgc ctacatccgg
     2041 tactcgccag ctactcagat tcatctattg caaaaatggg aaacaatctg gtgaaggcgc
     2101 ttgctgaagc gggttatgtg gatacgcccc gcagacgtaa cctgcaggca gtttaccttt
     2161 taccggaaac tcaggcagtg ttacagcgcc tgggacaaca ggacttgata tctattctgg
     2221 agggaaaacg gtgatagatc ccgttcttga atatcgcctg tctcaaatcc agagtcgcat
     2281 taacgaagat cgcttcctca aaaataacgg ctccggaaat gaaattggtt tttggatctt
     2341 tgattatccc gcgcagtgcg aactgcaggt acgggagcat ttgaaatatc tgctccggca
     2401 tctggaaaag gaccataaat ttgcctgtct gaatgtcttc caaatcatca tcgatatgct
     2461 caatgaacgc ggccttttcg agcgcgtctg ccagcaggaa gtcaaagtgg gtactgagac
     2521 gctgaaaaag cagcttgctg gtccgttaaa tcagaaaaag atcgctgatt ttatagcgaa
     2581 aaaagtcgat ctggctgccc aggattttgt cattcttacc ggcatgggca acgcctggcc
     2641 attagtacgc ggtcatgaac tgatgagtgc cttgcaggat gtcatggggt tcaccccact
     2701 gctgatgttt tatcctggca cctacagcgg gtacaacctt tccccgctca cagacaccgg
     2761 ttcacaaaat tattatcgcg ctttcagact ggtaccagat acgggacccg cagcaacatt
     2821 gaatcctcaa tgaagagcat aacaatgaat attgaacaga tttttgaaaa acctctaaaa
     2881 cgaaatataa acggggtagt caaagcagag caaaccgatg atgccagcgc gtacatcgag
     2941 ttagatgaat atgtcatcac ccgcgaactg gaaaaccatc ttcgccattt cttcgaatcc
     3001 tatgttcctg ccactggccc ggaacggatc cgtatggaaa acaagatcgg cgtatgggtt
     3061 tcaggcttct tcggttcagg taaatcgcac tttattaaga ttctttctta tcttttatct
     3121 aaccgcaaag ttacacataa cggtacggaa cgtaatgctt actccttctt tgaagataaa
     3181 atcaaagatg cattattcct tgccgatatt aacaaagcgg tgcattaccc gactgaagtc
     3241 attctgttca atattgattc gcgtgccaac gtagatgaca aagaagatgc cattcttaaa
     3301 gtcttcctga aagttttcaa cgaacgcatt ggatactgcg ctgattttcc gcatattgcc
     3361 catcttgagc gcgagctgga taaacgcggt cagtatgaaa cctttaaagc cgcgtttgcc
     3421 gatatcaatg gctcgcgctg ggaagacgag cgcgacgctt actacttcat cagcgatgac
     3481 atggcacaag cattaagcca ggccacgcag cagagtcttg aatcctcccg ccaatgggtg
     3541 gaacaactcg acaaaaactt cccgctggat atcaataatt tttgccagtg ggtaaaagag
     3601 tggctggatg acaatggtaa gaacatcctc tttatggtgg atgaagtcgg tcagttcatt
     3661 ggcaaaaata cgcaaatgat gctgaagctg cagactatta ctgaaaacct tggggtaatt
     3721 tgcggtggcc gcgcatgggt tatcgtgact tcgcaggccg atatcaacgc ggcaatcggt
     3781 ggtatgagca gtcgcgacgg acaggacttc tccaagatcc aggggcgctt ctctacacgc
     3841 ctgcaacttt ccagctctaa cacatcagaa gttatccaga aacgtttgtt ggtaaagact
     3901 gacgaagcaa aagcggcact ggcaaaagtg tggcaagaga aagccgatat cctgcgtaac
     3961 cagctggctt ttgaccctac aacaactact gcactacgtc cttttaccag cgaagaagag
     4021 ttcgttgaca actacccgtt tgtcccgtgg cactatcaga ttctgcaaaa agtgtttgaa
     4081 tctattcgaa cgaaaggtgc agcgggtaaa caattggcca tgggtgagcg ttctcagctg
     4141 gaggcattcc agacggcggc gcagcaaatc tcagcgcaag ggctggattc tctggtgcct
     4201 ttctggcgct tctatgccgc cattgagagc ttcctggaac ctgccgttag ccgcaccatc
     4261 actcaggctt gccagaatgg cattcttgat gaattcgatg gcaacctgct taaaacgctg
     4321 ttcctgatcc gctatgtgga aacgctgaaa agcaccctgg ataacctggt cacattgtct
     4381 atcgatagga tcgatgccga taaagttgag ttgcgccgcc gggtcgaaaa aagtctgaac
     4441 acgcttgaac gcctgatgct cattgcgcgc gttgaagata aatatgtgtt cctgaccaac
     4501 gaagaaaaag agatcgaaaa cgagatccgt aacgttgatg tcgatttctc tgcgatcaac
     4561 aaaaaactgg catcgatcat ctttgatgac attctgaaaa gccgtaaata tcgttatccg
     4621 gctaacaagc aagactttga tatcagtcgc ttcctgaacg gacacccatt agacggcgca
     4681 gtgcttaacg atctggtagt gaagatcctg acccctaaag atccgaccta ttcgttctat
     4741 aacagcgatg cgacctgtcg cccttatacg tcagaaggcg acggctgtat tttgattcgt
     4801 ctgcccgaag agggccgtac ctggagcgat attgatttag tcgtccagac tgaaaagttc
     4861 ctcaaagata acgccgggca acgtccggaa caggcaaccc tgctctcaga aaaagcgcgt
     4921 gaaaacagca accgggaaaa attactccgt gttcagttgg aatcactact tgcagaagca
     4981 gatgtctggg cgattggcga gcgcttaccg aaaaaatctt ccacaccatc gaacattgtc
     5041 gatgaagcct gccgttacgt gattgaaaac accttcggca agctgaagat gctgcggcct
     5101 tttaacggcg acatctcccg tgaaattcat gcattactga cggttgagaa cgacaccgaa
     5161 ctggatctcg gtaacctcga agagtccaac cccgacgcca tgcgcgaggt agaaacctgg
     5221 atcaacatga atatcgaata caataaacct gtgtatttac gcgatattct gaaccatttc
     5281 gcgcgtcgcc cttatggctg gcccgaagac gaagtgaaac tgctggtagc ccgtctggcc
     5341 tgcaaaggta agttcagctt tagccagcaa aacaacaacg tcgagcgaaa acaggcgtgg
     5401 gagttattta ataacagccg ccgccatagc gaattgcgtc tgcataaagt tcgccgtcat
     5461 gatgaagcgc aggtgcgtaa agccgcgcaa accatggctg acatcgctca gcagccgttt
     5521 aacgaacggg aagagccggc gctggttgaa catattcgtc aggtatttga agagtggaag
     5581 caagagctga acgtattccg cgccaaggca gagggcggaa acaatccggg gaaaaacgag
     5641 attgaatccg gtctgcgcct gcttaatgcc attcttaatg agaaagaaga ttttgccctg
     5701 atcgaaaaag tctcatcgct gaaagatgaa cttctggatt tcagtgaaga ccgtgaagat
     5761 ttggtcgact tctaccgtaa gcaattcgcc acctggcaaa aactgggtgc tgcgctgaat
     5821 ggcagcttta aatctaaccg cagcgcgctg gaaaaagacg ccgcagcggt taaagcgctg
     5881 ggtgagctgg aaagcatctg gcaaatgccg gaaccttata agcatctcaa tcgcatcacg
     5941 ccgttgattg aacaggtcca gaacgtcaac catcagttag tcgaacagca ccgccagcac
     6001 gccctcgaac gcattgacgc ccgcattgag gaaagccgtc aacgcttgct ggaagcgcac
     6061 gccacgtcag aactgcaaaa cagcgttctg ctgccgatgc aaaaagccag aaaacgcgct
     6121 gaggtcagcc agtcgattcc ggaaattttg gcggaacagc aagagacaaa agcgctgcaa
     6181 atggatgcag ataaaaagat taatctgtgg atcgatgagc tgtgtaaaaa gcaagaagca
     6241 caactccggg cagcaaatga agctaaacgc gctgccgact cagaacagac ttatgttgtg
     6301 gtggaaaaaa ccgttatcca accggtaccg aaaaaaacgc atctggtgaa tgtcgccagt
     6361 gagatgcgta acgccgccgg tggtgaggtt ctggaaacca ccgaacaggt ggaaaaggcg
     6421 ctcgacacgt tacgcacaac gctgctggcc gccattaaag caggcgatcg aattcgcctt
     6481 cagtaactcc catttcaggg cagcactctg ctgccctttg caggattttc tatgaatact
     6541 aataacatta aaaaatatgc cccacaggcc cgtaacgatt tccgcgatgc ggtgattcag
     6601 aagctaacga cgcttgggat cgctgcagat aaaaaaggca atttgcagat tgccgaggcc
     6661 gaaaccattg gcgagaccgt gcgttacggt cagtttgatt acccgttatc gacccttccc
     6721 cgccgcgaac ggctggtaaa acgcgcccgt gagcagggtt ttgaggtgct ggttgagcac
     6781 tgcgcctaca cctggtttaa ccgcttatgt gcaattcgct atatggagct acacggttat
     6841 cttgagcacg gcttccgtat gttgtcccac ccggagacgc cgaccgcgtt tgaggtgctg
     6901 gatcatgtgc cggaagtggc agaagccctg ctgccggaaa ataaggcgca gctggttgaa
     6961 atgaagcttt ccggtaatca ggacgaagcc ctgtaccgcg aactgctgct ggggcagtgc
     7021 cacgccctgc accacgtgat gccgttcctg tttgaagcgg tagatgacga agcggaactg
     7081 ctgttgccgg ataacctgac ccgtaccgac tctattctgc gtgggctggt tgataatatt
     7141 cctgaagaag actgggagca ggtagaggtt atcggctggc tgtatcagtt ctatatttcg
     7201 gaaaagaaag atgctgtgat tggcaaagtg gtgaagagcg aagatattcc tgccgccacc
     7261 cagctgttta cgccaaactg gattgtgcag tatctggtac agaactccgt tggccgccag
     7321 tggttgcaga cctacccgga ctcgccgctg aaagacaaaa tggagtacta cattgagcct
     7381 gcggagcaaa cgccggaagt gcaggcacag ctggcggcga ttaccccagc cagcattgaa
     7441 cccgaaagta ttaaagtgct cgacccggcc tgcggttccg gtcatatttt gattgaagcc
     7501 tataatgtgc tgaaaaatat ctacgaagag cgtggttatc gcgggcgtga tattccacaa
     7561 ctgattctgg aaaataatat ttttggtctt gatatcgacg accgcgcagc ccaactttcc
     7621 ggttttgcat tattaatgat ggcgcgtcag gatgaccgca gaatatttac ccgcgatgta
     7681 cgtctgaata ttgtctcttt gcaggaaagc ctgcatctgg atatcgccaa actctggcag
     7741 caactgaatt tccaccagca ggtacaaacc ggcagtatgg gggatatgtt tgctgaaaat
     7801 accgcgttaa cccaaactaa cagcgcagaa tatcagctgc tgatgcgcac gttgaaacgc
     7861 tttgtgaatg caaaaacgct gggatcgctg attcaggtgc cgcaggaaga agaagcggaa
     7921 ctgaaagtat ttctggacgc gctgtatcgc ctggaacagg aaggcgattt ccagcagaag
     7981 tcggcggcga aagcgtttat tccgtttatt cagcaggcgt ggattctggc acagcgatat
     8041 gatgcggtgg tggcgaatcc gccgtatatg ggaagcaaat accaatcatt aagtgtaaaa
     8101 aaacatctta aagataattt tactggttat gataaggatt tattttcagc ctttatagtc
     8161 agaaacttag aactttcaaa acaaggggct caacttggat tcatgacgcc atttgtctgg
     8221 atgtttatca ctacgtacga agaattaaga aaaaaactga tagagcaaga aattattaca
     8281 tcgcttgtgc agcttgaata ttcaggcttt gacggtgcaa ctgtgccaat ttgcacattc
     8341 acattacaga aaggtcatat taaccatttt actggtagct atatccgcct ctctgatttt
     8401 cgtggtgcat caaaccaagg tcccaaaact cttgaagcga tacaacacca taattgtgga
     8461 tggttttatg aagcgcagcc agacaatttt aaaaaaatac caggaacacc aatagcctat
     8521 tggctaggcc agaatataaa tattttcgat aatgacaaaa ta
//
LOCUS       DAZGLJ010000187         8463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000187 DAZGLJ010000000
VERSION     DAZGLJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.187"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1401
                     /gene="pglX"
                     /locus_tag="ACHK49_003825"
     CDS             <1..1401
                     /gene="pglX"
                     /locus_tag="ACHK49_003825"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFO9264389.1"
                     /translation="EKNGGIYPSKFRDKAGICWSKITSGQASFRVKSEDVEYDSASPV
                     IFNDKFNSDLYTLALLNTKVFNVLLKALNPTINTQVNDVMSLPFIKMEAAVQEKIETN
                     EQLLINIAMHDWNSQEISFDFMVNSLVSESRASSEVASSYAIARKKWQDAFNKTKELE
                     EENNHLVITSSNFNGINEELIDSEITLVSNPYYKYGDSTTNNETRLLSDSIADVLSYS
                     IGCMMGRYSLDREGLVYAHEGNKGFAELAAEGAYKTFPADNDGILPLMDDEWFEDDVT
                     SRVKEFVRTVWGEEHLQENLEFIAESLCLYAIKPKKGESALETIRRYLSTQFWKDHMK
                     MYKKRPIYWLFSSGKEKAFECLVYLHRYNDATLSRMRTEYVVPLLARYQANIDRLNDQ
                     LDEASGGEATRLKRERDSLIKKFSELRSYDDRLRHYADMRISIDLDDGVKVNYGKFGD
                     LLADVKAITGNAPEAI"
     gene            1578..4175
                     /gene="pglZ"
                     /locus_tag="ACHK49_003826"
     CDS             1578..4175
                     /gene="pglZ"
                     /locus_tag="ACHK49_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFO9264390.1"
                     /translation="MQNQDFIAGLKAKFAEHRIVFWHDPDKRFIEELEQLKLENVTLI
                     NMTHASQLAVKKRIEMDEPEQQFLLWFPHDAPPHEQDWLLDIRLYSSEFHADFAAITL
                     NTLGIPQLGLREHIQRRKAFFSTKRTQALKNLVTEQEDETSLDKKMIAVIAGAKTAKT
                     EDILFNLITQYVNQQTEDDSELENTQAMLKRHGLDPVLWEMLNHEMGYQAEEPSLENL
                     LLKLFCTDLSAQADRQQRAWLEKNVLLTPSGRASALAFMVTWRADRRYKEAYDYCAQQ
                     MQAALRPEDHYRLSSPYDLHECETTLSIEQTIIHALVTQLLEESTTLDREAFKKLLSE
                     RQSKYWCQTQPEYYAIYDALRQAERLLNLRNRHIDGFHYQDSATFWKAYCEELFRFDQ
                     AYRLFNEYALLVHSKGAMILKSLDDYIEALYSNWYLAELSRNWNEVLEAENRMQAWQI
                     PGVPRQQNFFNEMVKPQFQNPQIKRVFVIISDALRYEVAEELGNQINTEKRFTAELRS
                     QLGVLPSYTQLGMAALLPHEQLCYQPGNGDIVYADGLSTSGIPNRDTILKNYKGMAIK
                     SKDLLELKNQEGRDLIRDYEVVYIWHNTIDATGDTASTEDKTFEACRTAVTELKDLVT
                     KVINRLHGTRIFVTADHGFLFQQQALSVQDKTTLQIKPENTIKNHKRFIIGHQLPADD
                     FCWKGKVADTAGVSDNSEFLIPKGIQRFHFSGGAHFVHGGTMLQEVCVPVLQIKALQK
                     TAAEKQPQRRPVDIVAYHPMIKLVNNIDKVSLLQTHPVGELYEPRILNIYIVDNANNV
                     VSGKERISFDSDNSTMEKRVREVTLKLIGASFNRRNEYWLILEDAQTETGYQKYPVII
                     DLAFQDDFF"
     gene            4186..6270
                     /gene="brxL"
                     /locus_tag="ACHK49_003827"
     CDS             4186..6270
                     /gene="brxL"
                     /locus_tag="ACHK49_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFO9264391.1"
                     /translation="MQTHHDLPVSGVSAGEIASEGYDLDALLNQHFAGRVVRKDLTKQ
                     LKEGANVPVYVLEYLLGMYCASDDDDVVEQGLQNVKRILADNYVRPDEAEKVKSLIRE
                     RGSYKIIDKVSVKLNQKKDVYEAQLSNLGIKDALVPSQMVKDNEKLLTGGIWCMITVN
                     YFFEEGQKTSPFSLMTLKPIQMPNMDMEEVFDARKHFNRDQWIDVLLRSVGMEPANIE
                     QRTKWHLITRMIPFVENNYNVCELGPRGTGKSHVYKECSPNSLLVSGGQTTVANLFYN
                     MASRQIGLVGMWDVVAFDEVAGITFKDKDGVQIMKDYMASGSFSRGRDSIEGKASMVF
                     VGNINQSVETLVKTSHLLAPFPTAMIDTAFFDRFHAYIPGWEIPKMRPEFFTNRYGLI
                     TDYLAEYMREMRKRSFSDAIDKFFKLGNNLNQRDVIAVRRTVSGLLKLMHPDGAYSKE
                     DVRVCLTYAMEVRRRVKEQLKKLGGLEFFDVNFSYIDNETLEEFFVSVPEQGGSELIP
                     AGMPKPGVVHLVTQAESGMTGLYRFETQMTAGNGKHSVSGLGSNTSAKEAIRVGFDYF
                     KGNLNRVSAAAKFSDHEYHLHVVELHNTGPSTATSLAALIALCSILLAKPVQEQMVVL
                     GSMTLGGVINPVQDLAASLQLAFDSGAKRVLLPMSSAMDIPTVPAELFTKFQVSFYSD
                     PVDAVYKALGVN"
     gene            complement(6535..6960)
                     /locus_tag="ACHK49_003828"
     CDS             complement(6535..6960)
                     /locus_tag="ACHK49_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO9264392.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCGQHF
                     PGKKFCEACGTGIYSRAVCTKYVYSYQPDLFQDCGSAST"
     gene            complement(6957..7340)
                     /locus_tag="ACHK49_003829"
     CDS             complement(6957..7340)
                     /locus_tag="ACHK49_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO9264393.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
     gene            complement(7712..8311)
                     /locus_tag="ACHK49_003830"
     CDS             complement(7712..8311)
                     /locus_tag="ACHK49_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO9264394.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
BASE COUNT         2243 a         2073 c         2122 g         2025 t
ORIGIN      
        1 gaaaaaaacg gtggcattta tccatcaaaa tttagagata aagctggtat ttgttggtct
       61 aaaatcacat cgggccaagc tagctttaga gtcaaaagtg aagatgtaga atacgattct
      121 gcatcacctg taatatttaa cgataaattt aacagtgatc tttatacgct tgctctcttg
      181 aatacaaaag tttttaatgt tctacttaaa gcattgaacc ctactattaa tacccaagtt
      241 aatgatgtca tgtcattacc attcatcaaa atggaagcag ctgttcaaga aaaaatcgaa
      301 actaatgaac agctgctgat aaatatagcg atgcacgatt ggaattcgca agaaatttct
      361 tttgatttta tggttaattc tttagtcagc gaatcgagag catcatctga agtagcatca
      421 agctatgcta tagcaagaaa aaagtggcaa gatgctttta ataaaacgaa agagttggaa
      481 gaagaaaata accatttagt cattacaagt agcaacttta atgggataaa tgaagaatta
      541 attgattcag aaataacttt agtcagcaac ccatactaca agtacggtga ttcaacgact
      601 aataatgaaa ctcgtttgtt atcggattca atagcagatg tattaagtta ttccatcggc
      661 tgcatgatgg gtcgctactc cctcgatcgc gaaggcctcg tctacgccca tgaaggcaac
      721 aaaggctttg ccgaacttgc cgctgaaggt gcgtacaaaa ccttcccggc agacaatgac
      781 ggcatcctgc cgctgatgga tgacgagtgg tttgaggacg acgtcacctc tcgcgtcaaa
      841 gagtttgtcc gcaccgtctg gggcgaagag cacctgcagg aaaacctcga atttatcgcc
      901 gaaagtctct gtttatacgc gatcaagccg aaaaaaggcg aatctgcgct ggagaccatt
      961 cgtcgctatc tttccacaca gttctggaaa gatcatatga agatgtataa aaagcgccca
     1021 atctactggc tattcagctc cggtaaagag aaagccttcg agtgcctggt gtatctgcat
     1081 cgctataacg acgccacgct gtcgagaatg cgtaccgaat atgtggtgcc gctgctggca
     1141 cgttatcagg ccaatatcga tcgcctgaac gatcaacttg atgaagcttc tggcggtgaa
     1201 gccacacgcc tgaaacgcga acgcgacagc ctgatcaaaa aatttagcga actgcgtagc
     1261 tatgacgacc gcctgcgtca ctatgctgat atgagaatca gtattgatct cgacgatggc
     1321 gttaaggtta actacggcaa gtttggcgat ctgctggcag atgtcaaagc catcaccggc
     1381 aatgccccag aggcgatcta aaccagacgg cgcgttcccc tactgccggg ttctgcccgg
     1441 tggcaaatac caccgggaaa cgcgccgctg ctggcatttc tccacctcac ttcatgataa
     1501 aatgcgccac cgtgtcaaaa tctccttttc gcgttttggc gctttcttat tcatcgtaac
     1561 aacatgggat tgtgaacttg caaaatcagg actttattgc tggccttaaa gctaaatttg
     1621 ccgaacatcg catcgttttc tggcacgatc ccgataaacg ttttattgag gaactggaac
     1681 agctcaagct tgaaaacgtc acgctaatca acatgaccca cgcgtcacag ctggcggtga
     1741 aaaaacgcat cgagatggat gagccagaac agcagttcct gctgtggttc ccccatgatg
     1801 cgccgcctca tgaacaagac tggctgctgg atatccgcct ttacagcagc gaattccatg
     1861 ccgattttgc cgccatcacc ctgaacacgc tgggcattcc ccagcttggc ctgcgcgagc
     1921 atattcagcg acgcaaggcc ttcttcagca ctaaacgtac gcaggcgctg aaaaacctgg
     1981 tgacggaaca ggaagatgaa acctcgctgg ataagaaaat gattgcggtg atcgctggcg
     2041 caaagaccgc gaaaaccgaa gacattttgt ttaacctgat tacccagtac gttaaccaac
     2101 aaacagaaga cgacagcgaa ctggaaaaca cgcaggcgat gttgaaacgc cacggtctgg
     2161 acccggtatt gtgggaaatg ctcaaccacg aaatgggcta ccaggcagag gagccatcgc
     2221 tggaaaacct gctcctgaaa ctgttttgta ccgatctctc tgcccaggcc gacagacagc
     2281 agcgcgcctg gctggaaaaa aatgtcctgc tgacgccatc cggcagagca tccgccctgg
     2341 catttatggt gacctggcgt gccgatcgtc gctataaaga ggcttatgac tactgcgctc
     2401 agcaaatgca ggcggccctg cgcccggaag atcattaccg actcagttcg ccgtatgatt
     2461 tgcacgaatg cgaaaccacc ctcagcatcg aacaaaccat tattcatgcg ctggtaacgc
     2521 aactgctgga agagagcacc acgctcgatc gggaagcctt taaaaaactg ctctctgaac
     2581 gccagagcaa atactggtgc cagacacaac cagagtatta cgccatctat gacgcgctgc
     2641 gccaggctga gcgtttgctg aatctgcgca atcgccacat cgacggtttc cactatcagg
     2701 acagcgccac cttctggaaa gcctactgcg aagaactgtt ccgcttcgac caggcttatc
     2761 gcctgtttaa tgaatatgcc ctgctggttc acagcaaagg ggcgatgatc ctcaagagcc
     2821 tggatgatta tatcgaggca ctctacagca actggtatct ggcagaatta agccgtaact
     2881 ggaatgaagt gctggaagcg gaaaatcgta tgcaggcgtg gcaaattcct ggcgtgccgc
     2941 gtcagcagaa cttcttcaat gagatggtga agccgcagtt ccaaaatccg caaatcaaac
     3001 gcgtgttcgt gataatttcc gatgccctgc gttatgaagt ggcggaggag ctggggaatc
     3061 aaatcaatac cgagaaacgc tttaccgcag aactgcgatc gcagctcggc gtgctcccta
     3121 gctacaccca actgggaatg gcggcgttgc tgccccatga acaactttgc tatcaacccg
     3181 gtaacggcga catcgtttat gctgatgggc tgtcgacctc gggtattcct aaccgcgata
     3241 ccattctgaa gaactataag ggaatggcga taaaatcgaa ggaccttctg gagttaaaaa
     3301 accaggaagg gcgagacctt attcgcgatt acgaagtagt gtatatctgg cataatacga
     3361 ttgatgccac tggcgacacg gcatccacgg aagataaaac cttcgaagcg tgccgcacgg
     3421 cggtgactga actgaaagat ttagtcacca aggtaatcaa ccgtctccac ggcacacgca
     3481 tttttgttac ggcggatcac ggtttcctgt tccagcaaca ggcgctttcg gttcaggata
     3541 aaaccactct gcaaattaag ccggaaaaca ccatcaagaa ccacaaacgc tttattatcg
     3601 gccaccagct tcccgccgat gatttttgct ggaaagggaa agtggcggat accgcaggcg
     3661 tgagcgacaa cagcgagttc cttattccga aagggatcca gcgcttccat ttctctggcg
     3721 gcgcgcactt cgttcatggc ggcaccatgt tgcaggaggt ttgcgttccg gtattgcaga
     3781 taaaagccct gcaaaaaacc gccgcagaaa aacagccgca gcgccgcccg gtggatattg
     3841 tcgcttacca tccgatgatt aagctggtga acaatatcga taaagtgagc ctgttgcaga
     3901 cgcatccggt gggcgaactt tatgaaccgc gtatcctgaa catttacatt gtcgacaacg
     3961 ccaacaatgt ggtctcgggc aaagagcgca tcagctttga cagcgataac agcaccatgg
     4021 aaaaacgcgt acgcgaagtt acgctgaagc tgattggcgc tagcttcaac cgtcgcaatg
     4081 agtactggtt gatactggaa gacgcacaaa cggaaacggg gtatcagaag tacccggtca
     4141 ttatcgatct ggcgttccag gatgatttct tctaagtgag gcgatatgca aacccatcat
     4201 gatttacctg tttcaggcgt atccgcaggg gaaattgcct ccgagggtta cgatctggac
     4261 gccctgctga accagcattt tgctggtcgc gtggtgcgta aagatctcac caagcaactc
     4321 aaggaagggg caaacgtccc ggtgtatgtg cttgagtatc tgctcggcat gtactgcgcc
     4381 tctgacgatg acgacgtggt cgagcaaggg ttgcaaaacg ttaagcgtat tctggctgat
     4441 aactatgtgc gcccggatga agcggagaaa gtgaagtcgc tgatccgcga gcgtggttcg
     4501 tacaaaatca tcgataaagt gtcggtgaaa ctgaaccaga aaaaagacgt ttacgaagcc
     4561 cagctttcta acctcggcat caaagacgcg ctggtgccct cgcagatggt taaagacaac
     4621 gagaagctac tgacgggcgg tatctggtgc atgattaccg tcaactattt ctttgaagaa
     4681 gggcagaaga cctcaccctt ctcattgatg acgctcaagc ctatccagat gccgaatatg
     4741 gatatggaag aagtgttcga tgcgcgtaaa cactttaacc gtgaccagtg gatcgatgtg
     4801 ctgctgcgct cggtgggtat ggagcccgcc aatattgagc aacgcaccaa atggcacctt
     4861 atcacccgta tgatcccgtt cgtggagaac aactataacg tttgcgagct ggggccgcgt
     4921 ggcaccggta aaagccatgt gtataaagag tgttctccta actccctgtt agtttccggc
     4981 gggcaaacga ccgttgccaa cttgttctac aacatggcca gtcgccagat cggcctggtt
     5041 ggcatgtggg atgtggtagc gttcgacgaa gtcgcgggga tcactttcaa agataaagac
     5101 ggcgtgcaaa tcatgaaaga ttacatggcg tcaggatctt tctctcgcgg cagagattcg
     5161 attgaaggta aagcgtcgat ggttttcgtc ggcaacatca atcaaagcgt agagactctc
     5221 gttaaaacca gccatttgct ggcaccattt ccgactgcga tgattgatac agcatttttc
     5281 gaccgctttc atgcctatat tcccggttgg gaaatcccca aaatgcgccc ggaattcttt
     5341 accaaccgtt acgggctgat tacggattat ctcgctgaat atatgcgcga aatgcgcaaa
     5401 cgcagtttct ctgatgcgat tgataaattc tttaagctgg gtaacaacct caaccagcgt
     5461 gacgttattg ccgttcgacg taccgtgtcg gggttgttaa aactcatgca tcccgatggc
     5521 gcgtacagca aagaagatgt gcgagtctgc ctgacctatg cgatggaagt tcgccgccgc
     5581 gtgaaagagc aacttaaaaa actgggcggt ctggagttct tcgatgtgaa ctttagctac
     5641 atcgacaacg aaacgctgga agagtttttt gtgagcgtac cggaacaggg cggcagcgaa
     5701 cttattcctg ccggaatgcc aaagccgggt gttgtgcatc tggtcactca ggcagaaagc
     5761 ggcatgaccg ggctgtatcg ttttgaaaca cagatgactg ccggtaatgg taagcatagt
     5821 gtatcgggtc tgggttcaaa tacctccgcg aaagaagcta tccgcgtcgg tttcgattac
     5881 ttcaaaggca atttgaatcg ggtaagcgcg gccgcgaaat tctccgatca tgaatatcac
     5941 cttcatgtcg ttgaactgca taatactggc ccaagcaccg caaccagtct tgctgcgctt
     6001 atcgctttat gttcgatatt gctggcaaaa ccggtgcagg aacagatggt ggtgttgggc
     6061 agtatgacgc ttggtggggt aattaacccg gtgcaggatc ttgccgccag tttgcagctc
     6121 gccttcgaca gcggtgcaaa acgggttctg ttgccgatgt cctcggctat ggatattcca
     6181 acggttccgg cagagttatt taccaagttt caggtgagtt tttactcaga cccggttgat
     6241 gctgtttata aggcgctggg tgtgaattaa cgtagtaact attttaatga actggagagg
     6301 acaatggctc aagaacgttc tgtaattaaa ttcatcagac cttgcctctc cattcatgaa
     6361 tataaaaata cttacatctg agtattgttg gctgtgtttg aataaaaaga gagctgttca
     6421 aataagcctg tataactgtt ctttacaaaa aatggaggag ctatcagcta attcaacact
     6481 ggaagatgtt cgtcgagttg gattgaacgc cctgcgcaga ccaggcattt cggattaagt
     6541 gctcgcactt ccacagtcct ggaacaagtc tggctggtag ctgtaaacat actttgtaca
     6601 taccgctctg gaatagatcc ccgtgccaca ggcttcacag aactttttcc cgggaaaatg
     6661 ctgcccgcac atcacacaat gccactccag cacgaccggt aatggcgata aaaacatcgc
     6721 catatcctca atgtaagggt gggacttctc cggattcagc accacacagt ccgccttctg
     6781 ttgctcactc agggcatgta aatcatgctc aaaccacgcc ccctgagcat ctgtctgcag
     6841 aatcaaccgg ccatggcagg aaaggcagaa acactgccgg atatttctgc tgagcgcaga
     6901 gtcggcactg ataatgtgct cacccggcgt gatccccagc cccgttttta taccgttcat
     6961 tctgctcccc cctcctcact gaagcgcccg gtctggcagt gtgtgcagta ctgctcccca
     7021 taataatcgt ggtgacattg cctgcagtgc cagctggctt tacgtactac gggtaaggca
     7081 tccgggacga attgctgcag acgtttaatc aaccgtattt ctctgcgttc cggcctgaca
     7141 taagggcact catgaccgtg ctccgtcagc ccttcgtcag cgtgttcaaa ccagggccgt
     7201 tcagtgtcgt attgcgggtg gtatctgagt gcactgccgc aaaggtggca ggtgtagcta
     7261 tcgtaaggtg cagtctgtgc ggtacgggca ccggtcagac gtccgttgcc atcaagtgcg
     7321 agaaaagatt ttgcgtacat agtatatgtt cctctgcgcc agacgacacg caggcaacag
     7381 ccgtcccggt acgggcagcg tggtcagggt gtgaatggcg gttcagttaa aatctggact
     7441 ggagtttggg taaagcaggc tggttgctgt tacagcactg tggttgtcac acaatggccc
     7501 actgacgtaa aaaacagtca gtaagcagag atggagattg ggggatgtgc tgtatcggtt
     7561 gccggaagga atggtaaaga atgtgtcatg gttaaatctc cgtatggttc atacggaatg
     7621 accacgccgt aaaaaatgcg cagtgccgct gaagtggccg ccggaaccga cagcaaagat
     7681 tagcgccagc ccgtacttgt cgttcagggg tttacaccag agcacatatc cccggcaggg
     7741 ggctgtccag tcgtggtcga ccccggttac ttttttcagg aagcccgctg catttcagga
     7801 tgatgccaca ttctttctgt tcccgtttgg ccaggtagct gatggcatag cgtaaatcat
     7861 tcaccgcttt gtgatctgaa tagtgaatga cgtggttaat gtttaccgga taatttttat
     7921 taaaccggca ccagtggtag taaccattac cctcggtaac ccgcctccag atatctccca
     7981 tcaatctgga taccagatac gaactcctgt ggctctggcc gttcagatag cccacaaaat
     8041 gaatgtgaag ccctttacgt tctgtatatt ccatcaccca ggcatacccg accagtttct
     8101 ctcccacctc gttaatcagt tgcagcatct ccgcgcacat ggcgtacctg caggcacatc
     8161 tgtagctgag ggtgtttttt cggtaagcaa aatccattct gaacggtaac agtttcgaat
     8221 agaccccgaa taatgaatcc agatgctggt taaagtgctc caggagctgg ggagccattt
     8281 cgtaagcggg attggtgtta taagtttgca tgtttcttcc tcgtgttctg taacacagga
     8341 agaagcgttg ccgtaatttt tgaagaagat gttctcaggt agtagggtga acgtgtttcc
     8401 tccggtaacg acttccacgg gtatcagaac caggcaaaga acagcatacg taacagccat
     8461 aca
//
LOCUS       DAZGLJ010000188         8449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000188 DAZGLJ010000000
VERSION     DAZGLJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.188"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            149..3034
                     /locus_tag="ACHK49_003831"
     CDS             149..3034
                     /locus_tag="ACHK49_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO9264395.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINEGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTDYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQSNLFDQKQVNGGYRFSF"
     gene            complement(3134..5053)
                     /gene="dhaR"
                     /locus_tag="ACHK49_003832"
     CDS             complement(3134..5053)
                     /gene="dhaR"
                     /locus_tag="ACHK49_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO9264396.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPVDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            5281..6351
                     /gene="dhaK"
                     /locus_tag="ACHK49_003833"
     CDS             5281..6351
                     /gene="dhaK"
                     /locus_tag="ACHK49_003833"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO9264397.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            6362..6994
                     /gene="dhaL"
                     /locus_tag="ACHK49_003834"
     CDS             6362..6994
                     /gene="dhaL"
                     /locus_tag="ACHK49_003834"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO9264398.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7005..8423
                     /gene="dhaM"
                     /locus_tag="ACHK49_003835"
     CDS             7005..8423
                     /gene="dhaM"
                     /locus_tag="ACHK49_003835"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO9264399.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         2203 a         2128 c         2113 g         2005 t
ORIGIN      
        1 ttttgcccgg taatagagaa ttattctctg agaagctcat catgattgca ccatacaaat
       61 atcatgatga taagaggata ttattcattg atttaaacaa aatgacaaaa tccatagacc
      121 catagtctgg gtactatata ggagtggtat gggcatcaaa caacacaatg ggaataccaa
      181 agcagatcgt ctcgctgaat taaaaatccg ttcgccctca attcaactga taaaatttgg
      241 cgctattggt ttgaatgcaa ttatcttttc ccccctgctg atagctgctg atacaggaag
      301 tcaatatggc accaatatta ctattaatga aggtgacaga attacaggag ataccgccga
      361 tccatcagga aacctctatg gtgtaatgac cccagcagga aacacgcctg gcaatatcaa
      421 cctgggtaat gatgtcaccg tcaatgtcaa cgacgcctct ggatatgcaa aaggaatcat
      481 tattcagggc aaaaacagct ccctgacagc taaccgactc acagtagatg ttgttggtca
      541 aacctctgcc atcggcatta acttaattgg tgactatacc catgctgact taggcacagg
      601 cagcaccatt aagagtaacg atgacggcat cattattggg catagctcaa cactaacagc
      661 cactcaattc accattgaaa actcgaacgg tataggccta accatcaatg actatggcac
      721 cagtgtcgat cttggaagcg gaagtaaaat cacgaccgat ggaagtacag gtgtttatat
      781 cggtggtctc aacggcaata acgccaatgg tgctgcgcgt tttacggcta cagacctgac
      841 aatcgatgtt cagggctaca gcgccatggg gataaacgta cagaaaaact ctgttgtcga
      901 tctcggaaca aacagttcca ttaaaaccaa tggcgataat gcacacggcc tctggagctt
      961 tggccaggtt agcgcgaatg cactcactgt tgatgtaact ggagccgcgg ccaatggcgt
     1021 cgaagttcgt ggtggtacaa ccactatcgg tgcagatagc catatttctt ccgcgcaggg
     1081 cggtggcctc gtcaccagtg gttcagacgc gacaatcaat ttttctggca cggcagcgca
     1141 acgaaacagc atcttttccg gcggttctta tggtgcctcg gcccagacgg caacggctgt
     1201 tgtcaacatg caaaataccg atattacagt tgatcgtaat ggcagtctgg cgctgggttt
     1261 gtgggcgctc agcggcggta gaataaccgg agacagtttg gctatcaccg gcgcggcagg
     1321 agccagggga atttatgcca tgaccaacag ccagatcgac ctcacgagcg atctggtcat
     1381 tgatatgagt acacccgacc agatggccat tgcaacgcaa catgacgatg gttatgccgc
     1441 cagccgcatc aacgcctcgg gtcgtatgct tatcaacggt agcgttcttt ccaaaggtgg
     1501 gctaatcaat ctagatatgc acccagggtc ggtttggaca ggttcctccc tcagcgataa
     1561 tgtcaatggc gggaaactgg acgttgcaat gaataacagc gtctggaacg taacaagtaa
     1621 ttctaatctc gacacgctgg cgctgagcca ttcaactgtc gattttgcca gccacgggtc
     1681 aactgccggc acatttgcca cattaaacgt agagaacctg agcggtaaca gtacctttat
     1741 tatgcgtgct gatgttgttg gcgagggtaa tggcgttaat aataaagggg atttattgaa
     1801 tatcagcgga agtagtgctg gtaatcacgt attggctatc cgcaaccagg gcagcgaggc
     1861 cactacggga aatgaagttc tgacagtggt aaaaaccact gacggtgcgg cctcgttcag
     1921 cgcgtcttct caggttgagt tggggggata tctgtacgat gtgcgtaaaa atggcactaa
     1981 ctgggagctt tacgcttccg ggacagttcc ggaaccgact cctaatcctg aaccaacacc
     2041 ggctcccgct cagcctccca tagtcaaccc cgatcctacg cctgaacccg atcctacgcc
     2101 taaccccact cccacaccta aacccaccac gaccgcagat gctggcggca attatctcaa
     2161 tgtcggttac ttattaaact atgttgaaaa ccgtacgctg atgcaacgaa tgggtgacct
     2221 gcgaaatcag agtaaagacg gtaatatctg gctgcgcagt tatgggggaa gcctggactc
     2281 cttcgccagt ggcaaactga gcggctttga catgggttac agcggtatcc agtttggtgg
     2341 ggataaacgt ctctctgatg taatgccgtt gtatgtcggt ctgtatattg gctcaacaca
     2401 tgcatcgccg gactatagcg gaggcgacgg taccgcacgt tcagactaca tgggaatgta
     2461 tgccagttac atggcacaca acggttttta cagcgatctc gttgtaaaag catcgcgcca
     2521 gaaaaatagt tttcacgtac tggacagtca gaacaacggc gttaacgcca acggcactgc
     2581 gaatggattg agcatctccc tggaagccgg gcagaggttc aacctgaccc ctactggtta
     2641 tgggttctat atagagccgc aaacccagct cacatacagc caccagaatg agatggctat
     2701 gaaggcgagt aatggtctca atatacatct gaatcactac gaatcgctgc tggggcgtgc
     2761 cagcatgata ctgggatatg acatcaccgc aggcaacagc cagctgaata tgtatgtgaa
     2821 gactggagct atccgcgagt tttcagggga tacagactat ctgttgaaca actcccggga
     2881 gaagtacagt ttcaaaggta atggctggaa taacggcgtg ggagtcagtg cacagtataa
     2941 caaacagcac acattctacc tcgaagcgga ttacacgcag agtaacctct ttgatcagaa
     3001 gcaagtcaac ggaggatatc gcttcagctt ttaagtgcgg gatagaccag cccttaaacg
     3061 atgctgaaaa atgttagcgt gcgccggaat gaaatataga tcactctgat gtatcagaaa
     3121 tcgtctctgt ctttcatacc cggcgcttaa actgccctgc atcaatgcca tgttgcttca
     3181 ttttccgcca cagcgtagtg cggccgatcc caagtaaagc cgacatttcc tgaatgcgac
     3241 cgcctgtgac ctgggctgcg ttaataattg cctctttttc aacttccgca aatgacagac
     3301 tggtggaaag gcgtgtggcg ctgacatcat ctgttgcctg ctcggtaaac agatgttccg
     3361 gcaaatcact gacgcgaatg cgcccattat cactactcag agcaagattc tcgatgacgc
     3421 tgtaaagttc aaaatcgttg ccaggccatg cacaggaaac caggcgagcg agggcatcgt
     3481 catcaatttt cagccgcgta gagaagcgtt tttcaagact gcgtaatttg ttattcacca
     3541 gcgccggaat gctgccacgc cgcatacgca gaggcgggat ggtaatttca aatgcatgca
     3601 acgcgtaata cagctggcga ctaaaacgat tttgttccac cagcattgcg aggtccgcgg
     3661 tcgttgtggc aatcactttg acatcaactg gtattaaacg ccgcgcatcc agtcgcgtga
     3721 taacaccctg cttgataacc tgaagcaaag cagactgtaa ctccaccgcc agatattcaa
     3781 tcttttcaag aaacagcgtg ccgccgtgtg ccagttccag ccgactcaga cggccatttt
     3841 cattgtccgt gcgatcgcca ccaataaatt cttccgccag cgccgcatca ccatataact
     3901 cacaattgac ggcgatataa ggacctgcag cacgctcgct ttcattatga attgcctgac
     3961 ttagcagtgc ctttcccacg ccctcttctc cacaaagcag gacaggaaag ctactgcgcg
     4021 ccgcctggcg accaaaatga atcaagcggc gggtttgcgg atcgtcctgt ggcatatgag
     4081 cgaaggtatg gctgactttt cctaattgac tggtcatcaa ctgccgcatc tgttccacag
     4141 gatggagcaa caaaataaag ctggttccct gcgtttctat tatcggttta agggttatca
     4201 ccgcatcaat aaactgatgc tgactttcaa aggttgcttc tacgtgtttg agcggatgtg
     4261 cctgttttat tgcttgttgc aatacggcgg gtaacgttaa gagttcagtg attgcccttc
     4321 cctgacttgc cgtcgcgtca aggcgcaaga cccgcgccgc ctgggcatta ataaattgca
     4381 aattaccctg ctcgtcccag ctaatcacgc catcatccat actttctaac agggcattaa
     4441 gttgatttaa atgacggtta gtttcagcga gcaaactgtc cgtcagcagt aaatttccga
     4501 cctcgcgggc gattgccaac gtcaacggca aatcagctgc ggtagtttgc tcaaccggac
     4561 acgccagcgc tattgttccc gtcaatcggc ccttgctgtc aaacaacggc gttgcacaaa
     4621 aggcccagtt ccagagtgcc tgtttgaaat gttgatcggc catcgttttc acggcctgac
     4681 cagagatagc cgctaacgat agcgcacaag taccaataat tccctcggcg caatacgtgc
     4741 cgtcattgaa tcccagtgca cttagctgct gcaaggtttg cggatcgcca ttacggctga
     4801 gaatgcaggc ggtttcatcg aggataaaca gcgcacactc tcgcggtgcc atatattccc
     4861 aggcatcttc cagcgcagcc tgcccgagcg tcagcatcgc tttcttacgc cgataaatag
     4921 aagcaaatgt cacgccctgg gcctgatgtg gtacgttcca tgtctcccgt ttcatcagct
     4981 tattgcatcg ctcccaggag gttgcaatta agggagatat gccccgacca tcgttgttaa
     5041 aagcgccact catatcccgc atcccttatg acaaaaggtg ttccggctcg caattttaag
     5101 tattaataca cgtaaaagtt tcaatatgga acattcatta aagcatatgt ttcactatga
     5161 aacataattt gggctaattt tcttttccct tgcctgatgc acaatggatt catcggcagt
     5221 cagtggtcgc cgtgtcgttg aacatcatcc atgccctacc gtaattgctg gagcaaaata
     5281 atgaaaaaat tgatcaatga tgtgcaagac gtactggacg aacaactggc aggactggcg
     5341 aaagcgcatc catcgctgac actgcatcag gatccggtgt atgtcacccg agctgatgcc
     5401 cctgttgcag gaaaagtcgc cctgctgtcg ggtggcggca gcggacacga gccgatgcac
     5461 tgtggttata tcggtcaggg gatgctttcg ggggcctgtc cgggcgaaat tttcacctca
     5521 ccgacgcccg ataaaatctt tgaatgctcc atgcaaattg atggcggcga aggtgtactg
     5581 ttgattatca aaaattacac cggcgatatt cttaactttg aaacagcgac cgagttactg
     5641 cacgatagcg gcgtaaaagt gaccactgtg gtcattgatg acgacgttgc ggtaaaagac
     5701 agtctttata ccgccgggcg acgcggcgtt gccaacaccg tattaattga aaaactcgta
     5761 ggcgcagcgg cggagcgtgg cgactcactg gacgcctgtg cggaactggg gcgtaagttg
     5821 aataatcaag gccactcaat aggtatcgct ctcggtgcct gtaccgttcc tgccgcgggc
     5881 aaaccttctt ttaccctggc ggataatgag atggagtttg gcgtcggcat tcatggtgag
     5941 ccgggtattg accgccgctc cttctcttcc cttgatcaaa ccgtcgatga aatgttcgac
     6001 accctgctgg aaaatggctc ataccatcgc actttgcgtt tctgggatta tcaacaaggc
     6061 agttggcagg aagaacaaca aaccaaacaa ccgctccagt ctggcgatcg ggtgattgcg
     6121 ctggttaaca atcttggcgc aactccgctt tctgagctgt acggcgtcta taaccgcctg
     6181 accacacgtt gccagcaagc gggattgact atcgaacgta atttaattgg cgcgtactgc
     6241 acctcactgg atatgaccgg tttctcaatc accttactga aagttgatga cgaaacgctg
     6301 gcactctggg acgccccggt ccacaccccg gcccttaact ggggtaaata aggagaaagc
     6361 aatgtcactg agcagaactc aaattgttaa ctggctcact cgttgtggcg atattttcag
     6421 caccgagagc gagtatctta ccggactgga tcgcgaaatt ggcgacgctg accacgggct
     6481 aaatatgaac cgaggcttta gcaaagtggt ggaaaaactc cctgctatcg cagataaaga
     6541 tatcggtttc attctcaaga ataccggtat gacgctgctt tccagcgtcg gtggtgccag
     6601 tggtccgctg ttcggtacct tctttatccg cgccgcacag gcgacccagg cacggcaaag
     6661 cctgacactg gaagagcttt atcagatgtt ccgcgatggc gcggacggcg taatcagtcg
     6721 cgggaaagcc gaacctggcg ataaaaccat gtgtgatgtg tgggtgccgg tggtggaatc
     6781 gttacgtcag tccagcgagc aaaatctctc tgttccggtg gcgctcgaag ctgccagtag
     6841 aatcgccgaa tccgctgcac aaagtacgat tacgatgcaa gcccgcaaag gccgcgccag
     6901 ttatctcggt gaacgcagta ttggtcacca ggatcccggc gcgacctcgg tgatgtttat
     6961 gatgcaaatg ttggcgttag ccgcaaaaga gtaaggaatt ggtgatggta aacctggtca
     7021 tagtttcaca tagcagccga ctgggagaag gtgtcggtga attagcccgt cagatgttaa
     7081 tgagtgatag ttgtaaaatc gccattgccg cgggaattga cgatccacaa aatcctattg
     7141 gtaccgatgc cgtcaaagtg atggaggcca tcgaatctgt tgctgatgcc gaccatgtgc
     7201 tggtcatgat ggatatgggt agcgcattat tgagtgctga aactgcgctg gaattgctgg
     7261 ctcccaagat cgccgcaaaa gtacgtttgt gtgctgcgcc gttggtcgaa ggtacactgg
     7321 cagcaacggt cagcgcggcc tcgggagcgg atatcgacaa agttatcttt gacgctatgc
     7381 atgcgctgga agccaaacgt gaacaactgg gtttaccgtc ctccgacact gaaatctctg
     7441 acacatgtcc tgcgtacgat gaagaagccc gttctctggc ggtggtcata aaaaaccgta
     7501 acggcctgca tgtacgtccg gcctcccggc tggtttatac cttatcgaca tttaatgccg
     7561 atatgttgct ggaaaaaaac ggcaaatgcg tcacaccaga gagtattaac cagattgcgt
     7621 tactacaagt tcgctataac gatacgctgc gcctgattgc gaaagggcca gaagctgaag
     7681 aggcactgat cgctttccgt cagctggctg aagataactt tggtgaaacg gaggaagtcg
     7741 ctccacctac tctgcgtccc gttccgcctg tttcgggtaa agccttttat tatcaaccag
     7801 ttttatgtac ggtacaggca aaatcaaccc tgaccgtgga agaagaacaa gatcgattac
     7861 gccaggctat tgacttcacg ttattagatc tgatgacgtt aacagcgaaa gcagaagcca
     7921 gcgggcttga cgatattgcc gcaatctttt ctggtcacca tacactgtta gatgatccgg
     7981 aactgctggc ggcggcaagc gaactccttc agcatgaaca ttgcacggca gaatatgcct
     8041 ggcagcaagt tcttaaagaa cttagccagc aataccagca actggatgat gaatatctac
     8101 aagctcgcta tattgatgtg gacgatcttc tgcatcgcac cctggtccac ctgacccaaa
     8161 cgaaagaaga actcccgcag tttaactcgc caactattct actggcggag aacatttatc
     8221 cttccacagt actgcaactg gatccggcgg ttgtaaaagg tatctgcctt agcgccggaa
     8281 gtccggtatc ccacagcgcc ctaatcgccc gtgaactggg gattggctgg atttgccagc
     8341 agggtgagaa actgtatgcg atacaaccag aagaaacgct aacgctggac gttaaaacgc
     8401 aacgtttcaa ccgtcagggt taagccaccg ttcggatggc atcgttctg
//
LOCUS       DAZGLJ010000189         8437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000189 DAZGLJ010000000
VERSION     DAZGLJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.189"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(22..98)
                     /locus_tag="ACHK49_003836"
     tRNA            complement(22..98)
                     /locus_tag="ACHK49_003836"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(62..64),aa:Asp,seq:gtc)
     gene            complement(231..962)
                     /gene="dnaQ"
                     /locus_tag="ACHK49_003837"
     CDS             complement(231..962)
                     /gene="dnaQ"
                     /locus_tag="ACHK49_003837"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO9264400.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1027..1494
                     /gene="rnhA"
                     /locus_tag="ACHK49_003838"
     CDS             1027..1494
                     /gene="rnhA"
                     /locus_tag="ACHK49_003838"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO9264401.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1491..2213)
                     /locus_tag="ACHK49_003839"
     CDS             complement(1491..2213)
                     /locus_tag="ACHK49_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO9264402.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            2247..3002
                     /gene="gloB"
                     /locus_tag="ACHK49_003840"
     CDS             2247..3002
                     /gene="gloB"
                     /locus_tag="ACHK49_003840"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO9264403.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3074..4432
                     /gene="mltD"
                     /locus_tag="ACHK49_003841"
     CDS             3074..4432
                     /gene="mltD"
                     /locus_tag="ACHK49_003841"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO9264404.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4480..5250)
                     /locus_tag="ACHK49_003842"
     CDS             complement(4480..5250)
                     /locus_tag="ACHK49_003842"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO9264405.1"
                     /translation="MTIQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVVRMRTPEALID
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(5328..6128)
                     /locus_tag="ACHK49_003843"
     CDS             complement(5328..6128)
                     /locus_tag="ACHK49_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO9264406.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6369..7283
                     /gene="yafC"
                     /locus_tag="ACHK49_003844"
     CDS             6369..7283
                     /gene="yafC"
                     /locus_tag="ACHK49_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO9264407.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGQRYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIVRGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7280..8083)
                     /gene="dkgB"
                     /locus_tag="ACHK49_003845"
     CDS             complement(7280..8083)
                     /gene="dkgB"
                     /locus_tag="ACHK49_003845"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO9264408.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAVIGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8246..8322)
                     /locus_tag="ACHK49_003846"
     tRNA            complement(8246..8322)
                     /locus_tag="ACHK49_003846"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8286..8288),aa:Asp,seq:gtc)
BASE COUNT         2233 a         2030 c         2050 g         2124 t
ORIGIN      
        1 actcattttc atgagtgaat ttggcggaac ggacgggact cgaacccgcg accccctgcg
       61 tgacaggcag gtattctaac cgactgaact accgctccgc gttgtgttcc gttgggaacg
      121 aggcgaatat tacggattgc ctcacccttc gtcaacggtt tttctcatct tttgaatcgt
      181 ttgctgcaaa aatcgcccaa gccgctattt ttagcgcctt ttacaggtat ttatgcccgc
      241 cagaggcagc ttccgccctt cttctccacc agatcaagac gggcttcatg agctgcaagc
      301 tcttcatctg tcgcaaaaac aacgcgtaac ttacttgcct gacgtacaat gcgctgaatt
      361 gttgcttcac cttgttgctg ctgtgtctct ccttccatcg caaaagccat cgacgtttga
      421 ccaccggtca tcgccagata aacttccgca aggatctgtg catcgagtaa tgccccgtgt
      481 agcgttcgtt tactgttatc tatttcgtag cgggcacata acgcatcgag gctgttgcgc
      541 ttaccaggaa acattttcct cgccaccgca aggctatcgg tgaccttaca gaaggtattg
      601 gtcttcggaa tatcgcgctt aagcaacgaa aactcgtagt ccataaagcc gatatcgaac
      661 gctgcgttat ggatcaccaa ctccgcgccg cggatatagt ccatgaactc atcggctact
      721 tcggcaaacg tgggcttatc gagcaaaaat tcatcggcaa taccatgtac gccaaaggct
      781 tccggatcca ccagccgatc gggtttgaga taaacatgga agttattgcc cgtcaggcga
      841 cggttcacca cttcaacggc accaatctca atgatcttgt ggccttcata gtgcgcacca
      901 atctggttca taccggtggt ttcggtatcg agaacgatct ggcgtgtaat tgcagtgctc
      961 atagcggtca tttatgtcag acttgtcgtt ttacagttcg attcaattac aggaagtcta
     1021 ccagagatgc ttaaacaggt agaaattttc accgatggtt cgtgtctggg taatcctgga
     1081 cctgggggtt acggcgctat tttacgctat cgcggacgcg agaaaacctt tagcgcaggc
     1141 tacacccgca ccaccaacaa ccgtatggag ttgatggccg ctattgtcgc gctggaggcg
     1201 ttaaaagaac attgcgaagt cattttgagt accgacagcc agtatgtccg ccaaggtatc
     1261 acccagtgga tccataactg gaaaaaacgt ggctggaaaa cagcagacaa aaaaccagta
     1321 aaaaatgtcg atctctggca acgtcttgat gctgcattgg ggcagcatca aatcaaatgg
     1381 gaatgggtta aaggccatgc cggacacccg gaaaacgaac gctgtgatga actggcccgt
     1441 gctgcggcaa tgaatcccac actggaagat acaggctatc aagtcgaagt ttaagcctgt
     1501 ggtttacgat attgccgggt ggctccaacc gcctggcgaa ttcgtggctt gtttttactc
     1561 tgtttcatcg gatttagcgt taaaggaata gtccgtttcc gggcaacaat aagttgtaag
     1621 caaccaagcg caggaatatg tgcattcaat agttttccac cgtgtttgtt ccacgggaga
     1681 acgtggaaac ggctggcgtg cagcacttca aaattcaaca aagagagcca gtccagttgc
     1741 cgcatcagag taaacatccg gctgttatag ggcgaggttt ttcgcaatac cggcacaagt
     1801 ttgcgtaatc ccattaaact aatgggattg aagccactaa tcaccagcca gccatcatca
     1861 atcaataccc gatcggtttc acgcaataaa cgatgcggat cggtgcacca cggcaatgta
     1921 tgtgccagta gacaaacatc aacggattta tcggcaaaag gaagatgaag tggatccgcc
     1981 tgcacctgga cgggcattcc ttgcgcagaa acattcactt gatgagaaac cgcgcacgct
     2041 tcgcaattga tttctgcgct taaattgcca atcttaagca gatgaaaacc atacatttta
     2101 gtgaaccacg ggttgagctg gcgctccagc gccttgcgat aaagctctcc ccagggcaaa
     2161 tcgccccagc gatcaggagc cacgacagtt tgagggactc ttgccggttt catcacaacc
     2221 ttccgtttca cactaagagg taatctatga atcttaacag tattcccgcc tttgatgaca
     2281 attacatctg ggttttgaat gatgaagcag gtcgctgcct gattgtcgat cccggagacg
     2341 cagagccagt attaaacgcc attgccgcca ataactggca accggaggcc atatttctca
     2401 cccaccatca tcacgatcac gttggcggcg taaaagaact ggtggaaaag tttccgcaaa
     2461 ttgtggtgta tggtccacaa gagacacaag ataagggaac aacacaggta gtcaaagatg
     2521 gcgaaactgc cttcgttttg gggcatgaat ttagtgtaat tgccacaccg ggtcacactt
     2581 taggacatat ctgttacttc agtaaacctt atctattttg cggcgacacg ctgttttctg
     2641 gtgggtgtgg tagattgttt gaagggacag catcacaaat gtatcaatca cttaataagt
     2701 taagtgcgtt acctgacgat acattggtat gttgtgctca tgaatatacg ttatcaaata
     2761 tgaagtttgc tttgagtatt cttccgcacg atttgtccat aaatgattat tatcgtaaag
     2821 ttaaggagtt acgggcaaaa aatcaaataa cactacccgt aattctgaaa aatgagcggc
     2881 aaattaatgt ttttttaaga acggaagata ttgatttaat taatgtaatt aatgaagaaa
     2941 cattattgca acaacctgaa gagcgttttg catggttaag gtcaaagaaa gataggttct
     3001 gataaaactt tcttgtcatc ggctccgttc gccgttatga tcggtcgtct tttaagcaac
     3061 tattgacaca cacatgaagg caaaagcgat attactcgcc tctgtcctgc tcgtgggttg
     3121 ccagagtacc ggcaacgttc aacagcacgc acagagcctt tctgcagctg gtcaagggga
     3181 agcagcaaag tttacaagtc aggcacgatg gatggacgat gggacgtcta tcgcgccaga
     3241 tggtgacttg tgggctttca ttggcgacga gctaaagatg ggaattccgg aaaatgaccg
     3301 gattcgcgaa cagaaacaga aatatttacg caataagagc tatctccacg atgtaacttt
     3361 acgggcagag ccgtatatgt actggatagc agggcaagtt aaaaaacgta acatgcctat
     3421 ggaactggta ctactaccca tagtggagag cgcttttgat cctcacgcaa cgtctggcgc
     3481 caatgccgca ggcatctggc agatcattcc gagcacgggg cgcaattatg gtttgaaaca
     3541 gacccgcaat tatgacgcgc gtcgcgatgt tgttgcttca acaactgccg cgctgaatat
     3601 gatgcagcgt ctgaacaaga tgtttgacgg cgactggctt ctgaccgtag cggcttataa
     3661 cagcggcgaa ggtcgagtca tgaaggcaat taaaacgaac aaagcgcgtg ggaaatccac
     3721 ggacttctgg tcgttaccgt tgccgcagga aacgaagcag tacgtgccta aaatgctggc
     3781 attgagtgat attctcaaaa acagcaagcg ttatggcgta cgtctgccaa cgaccgatga
     3841 aagccgtgct ctggcgcgtg tgcacctgag cagcccggtt gaaatggcga aggttgcaga
     3901 tatggcgggg atttccgtca gcaagctgaa gacattcaac gctggcgtga aaggctccac
     3961 gctgggcgca agtggtccgc agtacgtgat ggtgccaaag aagcatgcag atcaactgcg
     4021 tgaatctctg gcttcaggcg aaattgctgc tgtacagtcg acgctggttg ccgacaatac
     4081 gccgcttaac agccgtgttt acaccgtacg ctctggcgac acgctttcaa gtatcgcttc
     4141 acgtctcggc gtaagcacca aagatttgca gcagtggaac aaactgcgcg gatctaaact
     4201 gaagccaggc caaagtttga cgattggcgc aggcagtagc gcacagcgac tggcaaacaa
     4261 cagcgatagc attacgtatc gtgtgcgcaa aggcgattcg ctttcaagca ttgctaaacg
     4321 ccacggcgtg aacatcaaag atgtgatgcg ctggaacagc gacactgcga atctgcaacc
     4381 aggcgataag ctgacgttgt ttgtgaaaaa caacaacatg ccagattcct gacaaaccag
     4441 ataataaaaa ggcaccgatt cccccggtgc cttttttatt tatgccgctt tatgtgcttc
     4501 taccatgatg atatcactgg tgaaagagcc gtcattctgc aaggcaaaat acgttctcac
     4561 ctctgtcgat gcgctctgtt ggtaaatgcg aatagcgtct attaacgctt ctggcgtacg
     4621 cattctcacg acccatgaag aaaattccag cggtaactta tctgtaatta aattatcaac
     4681 tatcagattg gcttcattga ttaacgtcaa ccactcaccg ctggcgtagt ttcgtacgtg
     4741 agaggtatcg cgtaatgctt ctaccgtctg taaccagatg tcgcgcactg ggtgacccgg
     4801 agacattacg tccatcacaa tcagcctacc gccaggtttc aatatccggt tcacttcacg
     4861 cagtgctgca ccaacatcat gccagtgatg ggcagaataa cggctgataa caatatcaaa
     4921 tgcgttgtcg gcaaatggca gactttcggc ataaccctgg cgggtggtga tatttttcag
     4981 ttgccggact tcggcagctt gtgccacgac atctagcatg tgggcagata agtcatacgc
     5041 caccaccgtg ctcacgtttt gcgcagcgac aaagctggca tgccctgctc cgcagcccat
     5101 atcaagtaca cttgcaccag gataatcagc cagacgcacc gccaggcgct gtaaatctcg
     5161 tccggatgca tgtacggtac tggtcagata ttcgctggct tgagagctaa actgcttttc
     5221 tacatggtca tggtgggatt gtattgtcat tgcgctgtcc ttttgttatt tgagtataaa
     5281 agggcagcgc caaacgttgg tctgccctgt ggcagacctg acatacctta tttatcaggc
     5341 ttgccgggac tgaattcaac gagtagcgga ttgtgatcgg aagcgcgcgt aaccagtaca
     5401 gaagcttcgc tgacgttcag accacggtag aaaacaaaat cgagcgggcg accaaacgcc
     5461 cgacggcgct gatcatcggt aaaacgcacc tggcgcagcg acatttcccg cgcaaagcga
     5521 tataacgcgt tcatccttct acggctccag gcattgaaat ctcccgccat aatgaccggg
     5581 ccgctgtggt gagctatctg atcaccaata ggaagtaact gcttactata gacatccacg
     5641 cccagactga agttgacggc gtgtatatta accaccatca acaggcgggt gtcaggtaat
     5701 ggatagaccg tcaccagtgc cgacttcgcc agacgcaaaa tgggttctcg ttcacgtaac
     5761 gggcagcaat acactggatg cgccgccgaa agggtcatta caccggaagg atgttgtggc
     5821 agcacgaaag cgggcacctg atcggcggca agatagttag cggtcgcaaa ctgtactaac
     5881 tctggcgttg tctgcgcttc ctgcaataac accagatgtg catctttgcc gtagttcttt
     5941 aataccgaca accattcagc gcgttgctgt ttgtatatgt tccacaccag gatccgaata
     6001 cgctcttcag tacttaacgg ttctccaggt ggtaatgcct ggccgatgct cgcaaaagac
     6061 cccggcggta agatcctttc cgcaggttgt ccggcaacat agcgcatggc ataggtgttt
     6121 tttcgcactt ttgactttga aacctctatt acgtcaaccc ttccgcactc gggagggttc
     6181 tactgttata gggattttag ctcactcttt caacgagcaa ttgtatattg ttatgtaagc
     6241 aagtgcttac acaggtcaga attttgctca ttattgagaa cattaaatcg gatgacatat
     6301 cacgcatcct gatggtttaa tgacaaataa attcaaaata tctcttctat tttgcaacag
     6361 gagcaaaaat gaaagccacg tcggaagaac tcgccatttt tgtttcggtc gtagaaagcg
     6421 gcagctttag ccgggcagcg gaacaattag ggcaagcaaa ctcagcggta agccgggcag
     6481 tgaaaaagct ggagatgaaa cttggcgtta gcctgcttaa tcggaccacg cgacaactta
     6541 gcctgacgga agaaggccag cgttattttc gtcgcgtaca gtcaattttg caggagatgg
     6601 cagcggcaga atcagaaatt atggagacgc gtaatacacc gcgtggactg ttacgcatcg
     6661 atgccgcaac tccagtggtg ctgcactttc tgatgccgtt aattaagcct ttccgtgaac
     6721 gctatccgga agttacttta tcgctagtct cctccgaaac gattattaat ttgatcgaaa
     6781 gaaaagtgga tgtcgcgata cgcgctggta cgttaacgga ttccagctta cgtgccaggc
     6841 cgttatttaa cagttatcga aaaattatcg cctcccccga ttatatttcc cgctacggga
     6901 agccagaaac gatcgacgat ttaaagcaac atgtttgcct gggattcact gaacccgctt
     6961 ccctcaatac ctggccgata gcctgtagtg atggacaatt acatgaggtg aagtacggtt
     7021 tgtcatccaa tagtggggaa acactgaaac agctttgcct gagtggcaac gggattgcgt
     7081 gtttgtccga ctacatgatc gacaaagaaa tcgttcgcgg agaattggtg gagttaatgg
     7141 cagataaagt gttgccagtg gaaatgccat tcagtgcagt ctattacagc gaccgtgcgg
     7201 taagtacgcg catccgggct tttatcgatt tccttagcga gcatgtaaaa acagctcccg
     7261 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
     7321 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
     7381 gtgccttaag attactttcc aggttttcac gtttagtaga agaaggaatt actgagtaac
     7441 cttcccccat agcccacgcc agaatcactt gtgccggagt cgcattgtgt ttagctgcga
     7501 tacgagcaat aacctcatct ttcaaggctt tcccataagc cagcgtcata taggaagtaa
     7561 tatggatgcc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
     7621 gttcaatctg gttagtagcg atgttttcag cgccaataac agcaatcgcc ttttccatca
     7681 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
     7741 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
     7801 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga cttgggatca
     7861 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
     7921 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
     7981 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
     8041 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
     8101 gctattttcg ttaactgagg tgattatggg cttcagactg ttgaggaaaa agagcgtaaa
     8161 atgcagagga tttttgcgat tctggcaata atagatatcc agacgaaaaa aaacctcgag
     8221 cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc
     8281 tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac
     8341 caccggtttt atgaccggct tactgcttaa tttgatgcct ggcagtttat gaatcacttc
     8401 gtgattcacc cttcgggccg ttgcttcgca acgttca
//
LOCUS       DAZGLJ010000190         8325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000190 DAZGLJ010000000
VERSION     DAZGLJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.190"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..142
                     /locus_tag="ACHK49_003847"
     CDS             <1..142
                     /locus_tag="ACHK49_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO9264409.1"
                     /translation="YNIAPFNKDLWSLLKSSQECPPGESKITNKCLHNSCIIKICPYG
                     LK"
     gene            complement(245..1495)
                     /gene="icd"
                     /locus_tag="ACHK49_003848"
     CDS             complement(245..1495)
                     /gene="icd"
                     /locus_tag="ACHK49_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO9264410.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1667..2320
                     /gene="rluE"
                     /locus_tag="ACHK49_003849"
     CDS             1667..2320
                     /gene="rluE"
                     /locus_tag="ACHK49_003849"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO9264411.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2330..2791
                     /gene="nudJ"
                     /locus_tag="ACHK49_003850"
     CDS             2330..2791
                     /gene="nudJ"
                     /locus_tag="ACHK49_003850"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO9264412.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2845..3951
                     /gene="mnmA"
                     /locus_tag="ACHK49_003851"
                     /gene_synonym="trmU"
     CDS             2845..3951
                     /gene="mnmA"
                     /locus_tag="ACHK49_003851"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO9264413.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3987..4628
                     /gene="hflD"
                     /locus_tag="ACHK49_003852"
     CDS             3987..4628
                     /gene="hflD"
                     /locus_tag="ACHK49_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO9264414.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4632..6002
                     /gene="purB"
                     /locus_tag="ACHK49_003853"
     CDS             4632..6002
                     /gene="purB"
                     /locus_tag="ACHK49_003853"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO9264415.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6170..6841
                     /gene="phoP"
                     /locus_tag="ACHK49_003854"
     CDS             6170..6841
                     /gene="phoP"
                     /locus_tag="ACHK49_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO9264416.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6841..8301
                     /gene="phoQ"
                     /locus_tag="ACHK49_003855"
     CDS             6841..8301
                     /gene="phoQ"
                     /locus_tag="ACHK49_003855"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO9264417.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
BASE COUNT         2078 a         2101 c         2113 g         2033 t
ORIGIN      
        1 ttataacatc gcaccattca acaaggattt atggagtcta ctcaaaagca gtcaagagtg
       61 tcctccagga gaaagcaaaa taacaaataa atgtttgcat aatagttgca ttataaaaat
      121 atgcccatat gggctcaagt aaagggtttt atataaatat gctcgatatg ctattaataa
      181 tacctacata aataaaacaa cgggcgtgtt atacgcccgt tgcaatatta aacacatgta
      241 gtgattacat gttcttgatg atcgcatcac caaattctga acatttcagc agtttagcgc
      301 cttccatcag acgctcgaag tcataggtta cggtcttcgc attgattgcg ccttccatac
      361 ctttaacaat caggtctgcg gcttcagtcc agcccatatg gcgtaacatc atctcagcgg
      421 agagaataat agagccaggg tttactttgt cctgaccggc gtatttcggc gcagtaccgt
      481 gggtggcttc aaacagggcg cattcgtcac cgatgtttgc tccaggggcg ataccgatac
      541 cgccaacctg cgctgccaga gcgtcagaaa tgtagtcacc gttcaggttc atacaggcga
      601 taacatcata ttcagccgga cgcagcagga tctgttgcag gaatgcatca gcaatcacgt
      661 ctttaatgac gatctctttg ccggtgttcg ggtttttaac tttcagccac gggccgccgt
      721 cgatcagttc accgccaaac tcttcacgcg ccagctggta gccccagtct ttaaacgcgc
      781 cttcggtgaa cttcatgatg ttgcctttgt gcaccagagt cacagagtca cgatcgttag
      841 caattgcgta ttcgatcgct gcacgaacca gacgtttggt gccttcttcc gaacacggct
      901 taataccgat accacaatgt tccgggaagc gaattttctt cacccccatc tcttcacgca
      961 ggaatttaat cactttctcg gcgtcggcag agtctgcttt ccattcgata cccgcataaa
     1021 tgtcttccga gttttcacgg aagataacca tatcggtcag ttcagggtgt ttaaccgggc
     1081 ttggagtgcc ttgatagtaa cgtaccggac gcaggcagat gtagagatcc agttcctggc
     1141 gcagggcaac gttcagagag cgaataccgc caccaacagg agtggtcagc ggacctttaa
     1201 tggcaacgcg atattcacga atcagatcaa gagtttcagc aggcagccag acgtcctgac
     1261 cataaacctg tgtggatttt tcaccggtgt aaatttccat ccaggagatt ttacgctcgc
     1321 ctttataggc tttctcgact gcagcgtcga ccactttcag catggctggg gttacatcta
     1381 caccgattcc atcaccttca atgtaaggga taatcggatt ttcaggaacg ttgagtttgc
     1441 cgttttgcag ggtgatcttc ttgccttgtg ccggaacaac tactttactt tccattcacc
     1501 tctccttcga gcgctactgg tttgctcgtc tgccaccacg ttgcatatgc gtttgcgtcc
     1561 tgcgatacgg atgctttaga gcaatttttt gttaatgatt tgtaattggc ttgtcaatac
     1621 taccctattg tttgccgtca tgaaagatgc gcgttattag gctataatgc ggcaattcat
     1681 aatctctgaa aataccatgc aaaaaacttc ttttagaaat caccaagtta agcgattcag
     1741 ctcgcaacgt tctaccaggc gtaaacctga aaaccagccc acgcgtgtga tcctgttcaa
     1801 taaaccctac gatgttcttc cgcagttcac cgatgaagcc ggacgcaaaa cattaaaaga
     1861 attcatcccg gttcagggcg tttatgcagc aggtcgtctt gaccgcgata gcgaagggtt
     1921 actggtgctg accaataacg gcgcgttgca ggcgcgttta acccagccgg gcaaacgcac
     1981 cgggaaaatc tattatgtgc aggtggaagg tattcccaca caagacgcac ttgaagcctt
     2041 gcgcaatggc gtaaccttaa atgatggccc taccctgccc gcaggcgcgg agctggttga
     2101 cgaacccgcg tggttatggc cgcggaatcc accaattcgt gaacgcaaaa gtattcccac
     2161 cagctggctg aagatcacct tatatgaagg acgtaatcgc caggtgcgcc gcatgaccgc
     2221 ccatgttggt ttccccacgc tgcgactgat tcgctatgcg atgggagatt actctttgga
     2281 taatcttgcc aatggcgaat ggcgagaagt gacagattaa ggatgcaaaa tgtttaaacc
     2341 gcacgttacc gttgcttgcg tggtgcacgc agaaggcaaa tttttagtcg ttgaagagac
     2401 gattaatggt aaagcgttat ggaaccaacc tgccgggcat ctggaagccg atgaaacctt
     2461 agtggaagcc gccgcccgtg aactgtggga agaaaccggc atcagcgcgc agccgcaaca
     2521 ctttattcgt atgcatcagt ggattgcgcc agataaaacg ccgtttttgc gtttcctctt
     2581 tgccattgag cttgagcaaa tatgcccgac ccagcctcat gacagcgata tcgactgctg
     2641 tcgttgggtc agcgccgaag aaattttaca ggcgtcaaat cttcgttcgc cgctggtggc
     2701 ggaaagtatc cgttgttatc aaagcgggca acgctatccg ctggagatga ttggcgattt
     2761 taactggcct tttacaaagg gtgtcatcta aaaggctgcg tgatagaata cgccgccttg
     2821 aagttcaatg tcgtgagtga tccaatgtct gaaaccgcaa aaaaagtaat cgtcggcatg
     2881 tccggcggtg tcgattcctc cgtttctgcc tggctgttgc aacaacaggg atatcaggtc
     2941 gaaggcctgt ttatgaagaa ctgggaagaa gacgacggtg aggaatattg cacagcggca
     3001 gcggatctgg ctgatgccca ggctgtctgc gacaagctcg gcattgaact gcacaccgtt
     3061 aactttgctg ccgagtactg ggacaacgtc ttcgaactgt tccttgccga atataaagcc
     3121 ggtcgcacgc cgaatccgga tattctgtgc aacaaagaga tcaaatttaa agccttcctc
     3181 gaatttgccg ccgaagattt aggtgccgat tatatcgcta ccggtcatta cgtacgtcgg
     3241 gccgatgtcg atggcaagag ccgcctgctg cgtggtctgg acagcaataa agaccagagc
     3301 tacttccttt atacgctcag ccatgagcag attgcgcaaa gcctgttccc ggtcggcgaa
     3361 ctggaaaaac cgcaggtgcg taagattgct gaagatcttg gtctggtcac cgcgaagaaa
     3421 aaagactcta ccggcatttg cttcattggc gaacgtaaat tccgcgagtt cctgggccgt
     3481 tatctcccgg cgcaaccggg caaaatcatt accgtcgatg gcgatgaaat tggtgagcac
     3541 caggggctga tgtatcacac tctcggtcag cgtaaaggtc tgggtatcgg tggcaccaaa
     3601 gaaggtaccg aagaaccgtg gtatgtggtg gacaaagacg tcgaaaacaa cattctggtt
     3661 gtcgctcagg gccatgaaca cccgcggctg atgtctgtcg ggttgattgc ccagcagttg
     3721 cactgggtcg atcgcgaacc attcaccggc actatgcgtt gcacggtaaa aacccgctat
     3781 cgccagaccg atatcccttg caccgtcaag gcgctggacg atgatcgcat tgaagtgatt
     3841 ttcgatgaac cggttgccgc cgtgacgccg ggccagtctg ccgtcttcta taacggtgaa
     3901 gtgtgcctcg gtggcggtat tattgagcag cgtctgccgc tgccggtctg attattatct
     3961 ttacttaaaa caaggaagca gtgaacgtgg caaagaatta ctatgacatc accctcgccc
     4021 tggccggtat ttgtcagtcg gcacgcctgg tgcaacaact cgctcaccag gggcattgtg
     4081 atgccgatgc gctacacgtc tcactcaaca gtattattga tatgaacccc agctcgacgc
     4141 tggcggtttt tggcggtagc gaagccaacc tgcgcgtcgg gctggaaacc ctgctcggcg
     4201 tgctcaatgc cagcagtcgc cagggcttaa acgccgaatt aacccgctac acactcagct
     4261 tgatggtgct tgagcgcaaa ctctcctcag cgaaaggcgc gctcgacact ctgggcaacc
     4321 ggatcaacgg cctgcaacgc cagctcgaac acttcgattt acagtccgaa acgctgatga
     4381 gcgcgatggc tgctatctat gttgatgtga tcagcccgct tggcccgcgc attcaggtca
     4441 ccggttcccc tgctgtactg caaagcccac aagtgcaggc gaaagttcgc gcaaccctgc
     4501 tggcaggcat tcgcgccgcc gtgctctggc accaggtcgg cggcggacgt ctgcaactga
     4561 tgttttctcg taatcgcctg accactcagg caaaacaaat tcttgctcat ttaaccccgg
     4621 agttgtgatc tatggaatta tcctcactga ccgccgtttc ccctgtcgat ggacgctacg
     4681 gcgataaagt cagcgcgctg cgcgggattt tcagcgaata tggtttgctg aaattccgtg
     4741 tacaagttga agtacgttgg ctgcaaaaac tggccgcgca cgcagcgatc aaggaagttc
     4801 ctgcttttgc tgccgacgca atcggttacc ttgatgcaat tgtcgccaat ttcagcgaag
     4861 aagatgccgc acgcatcaaa accatcgagc gtaccactaa ccacgacgtt aaagcggttg
     4921 agtatttcct gaaagaaaaa gtggcggaga tcccggaact gcacgcggtt tctgaattca
     4981 tccactttgc ctgtacttcg gaagatatca ataacctctc ccacgcatta atgctgaaaa
     5041 ccgcgcgtga tgaagtgatc ctgccgtact ggcgtcaact gattgatggc attaaagatc
     5101 tcgccgttca gtaccgcgat atcccgctgc tgtctcgtac ccacggtcag ccagccacgc
     5161 cgtcaaccat cggtaaagag atggcaaacg tcgcctaccg tatggagcgc cagtaccgcc
     5221 agcttaatca ggtggagatc ctcggcaaaa tcaacggcgc ggtcggtaac tataacgccc
     5281 atatcgccgc ttacccggaa gttgactggc atcagttcag cgaagagttc gtcacctcgc
     5341 tgggtattca gtggaacccg tacaccaccc agatcgaacc gcacgactac attgccgaat
     5401 tgtttgattg cgttgcgcgc ttcaacacca ttctgatcga ctttgaccgt gacgtctggg
     5461 gttatatcgc ccttaaccac ttcaaacaga aaaccattgc tggtgagatt ggttcttcca
     5521 ccatgccgca taaagttaac ccgattgact tcgaaaactc cgaagggaat ctgggccttt
     5581 ccaacgcggt attgcagcat ctggcaagca aactgccggt ttcccgctgg cagcgtgacc
     5641 tgaccgactc caccgtgctg cgtaacctcg gcgtgggtat cggttatgcg ctgattgcgt
     5701 atcaatccac cctgaaaggc gtgagcaaac tggaagtaaa ccgtgaccat ctgctggatg
     5761 agctggatca caactgggaa gtgctggctg agccaatcca gacagttatg cgtcgctatg
     5821 gcatcgaaaa accgtacgag aagctgaaag agctgactcg cggtaagcgc gttgacgccg
     5881 aaggcatgaa gcagtttatc gatggtctgg cgttgccaga agaagagaaa gcccgcctga
     5941 aagcgatgac gccggctaac tatattggtc gagctatcac catggttgat gagctgaaat
     6001 aaactcgtat cagtgccgga tggcgatgct gtccggcctg tttattaaaa ttatccactt
     6061 tttctttttt cactttacct cccctccccg ctggtttatt taatgtttac ccccataaac
     6121 acataatcgc gttacactat tttaataatt aagacaggga gaaataaaaa tgcgcgtact
     6181 ggttgttgaa gacaatgcgt tgttacgtca ccaccttaaa gttcagattc aggatgctgg
     6241 tcatcaggtc gatgacgcag aagatgccaa agaagccgat tattatctca atgaacattt
     6301 accggatatt gcgattgtcg atctcggatt gcctgacgag gacggtctgt cactgattcg
     6361 ccgctggcgt agcaatgatg tttcactgcc gattctggta ttaaccgccc gtgaaagctg
     6421 gcaggacaaa gtcgaagtat taagtgccgg tgctgatgat tacgtgacta aaccgtttca
     6481 tattgaagag gtgatggcgc gaatgcaggc attaatgcgg cgtaatagcg gcctggcttc
     6541 acaggtcatt tcgctacccc cgtttcaggt tgatctctct cgccgtgaat tatctattaa
     6601 tgacgaagtg atcaaactga ccgcgttcga atacaccatt atggaaacgt tgatacgcaa
     6661 taatggcaaa gtggtgagca aagattcgtt aatgctccaa ctctatccgg atgcggagct
     6721 gcgggaaagc cataccattg atgtactgat gggacgtctg cgcaaaaaaa ttcaggcaca
     6781 atatccgcaa gaagtgatta ctaccgttcg cggccagggc tatttgttcg aattgcgctg
     6841 atgaaaaaat tactgcgtct ttttttcccg ctctcactgc gggtacgttt tctgttggca
     6901 acggctgcgg tagtactggt gctttcgctt gcctacggaa tggtcgcgct gatcggttat
     6961 agcgtcagtt tcgataaaac tacgtttcgg ctgttacgtg gcgagagcaa tctgttctat
     7021 acccttgcga agtgggaaaa caataagttg catgtcgagt tacccgaaaa tatcgacaag
     7081 caaagcccca ccatgacgct aatttatgat gagaacgggc agcttttatg ggcgcaacgt
     7141 gacgtgccct ggctgatgaa gatgatccag cctgactggc tgaaatcgaa tggttttcat
     7201 gaaattgaag cggatgttaa cgataccagc ctcttgctga gtggagatca ttcgatacag
     7261 caacagttgc aggaagtgcg ggaagatgat gacgacgcgg agatgaccca ctcggtggca
     7321 gtaaacgtct acccggcaac atcgcggatg ccaaaattaa ccattgtggt ggtggatacc
     7381 attccggtgg agctaaaaag ttcctatatg gtctggagct ggtttatcta tgtgctctca
     7441 gccaatctgc tgttagtgat cccgctgctg tgggtcgccg cctggtggag tttacgcccc
     7501 atcgaagccc tggcaaaaga agtccgcgaa ctggaagaac ataaccgcga attgctcaat
     7561 ccagccacaa cgcgagaact gaccagtctg gtacgaaacc tgaaccgatt gttaaaaagt
     7621 gaacgcgaac gttacgacaa ataccgtacg acgctcaccg acctgaccca tagtctgaaa
     7681 acgccactgg cggtgctgca aagtacgctg cgttctctgc gtagtgaaaa gatgagcgtc
     7741 agtgatgctg agccggtaat gctggagcaa atcagccgca tttcacagca aattggctac
     7801 tacctgcatc gtgccagtat gcgcggcggg acattgctca gccgcgagct gcatccggtc
     7861 gccccactgc tggacaatct cacctcagcg ctgaacaaag tgtatcaacg caaaggggtc
     7921 aatatctctc tcgatatttc gccagagatc agctttgtcg gtgagcagaa cgattttgtc
     7981 gaggtgatgg gcaacgtgct ggataatgcc tgtaaatatt gcctcgagtt tgtcgaaatt
     8041 tctgcaaggc aaaccgacga gcatctctat attgtggtcg aggatgatgg ccccggtatt
     8101 ccattaagca agcgagaggt cattttcgac cgtggtcaac gggttgatac tttacgccct
     8161 gggcaaggtg tagggctggc ggtagcccgc gaaatcaccg agcaatatga gggtaaaatc
     8221 gtcgccggag agagcatgct gggcggtgcg cggatggagg tgatttttgg tcgccagcat
     8281 tctgcgccga aagatgaata aatatgtcct tttatcatta cacca
//
LOCUS       DAZGLJ010000191         8218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000191 DAZGLJ010000000
VERSION     DAZGLJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.191"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(383..1144)
                     /gene="envY"
                     /locus_tag="ACHK49_003856"
     CDS             complement(383..1144)
                     /gene="envY"
                     /locus_tag="ACHK49_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO9264418.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1327..2217)
                     /locus_tag="ACHK49_003857"
     CDS             complement(1327..2217)
                     /locus_tag="ACHK49_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO9264419.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2218..5190)
                     /gene="nfrA"
                     /locus_tag="ACHK49_003858"
     CDS             complement(2218..5190)
                     /gene="nfrA"
                     /locus_tag="ACHK49_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO9264420.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAAQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5177..7414)
                     /gene="nrfB"
                     /locus_tag="ACHK49_003859"
     CDS             complement(5177..7414)
                     /gene="nrfB"
                     /locus_tag="ACHK49_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO9264421.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMFNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            complement(7683..>8218)
                     /locus_tag="ACHK49_003860"
     CDS             complement(7683..>8218)
                     /locus_tag="ACHK49_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO9264422.1"
                     /translation="LNKAFEGKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVPD
                     ELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHW
                     HVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKA
                     AMDRNYLASVLAGSGLS"
BASE COUNT         1916 a         2089 c         2129 g         2084 t
ORIGIN      
        1 gtttttggtt taattatctg ttattgtttt atgttgcggt tttactgtgt ggttttttat
       61 gcttttgtgg tgtttttatc tatttaagcg ccatgccttt aaaggcatat aagcgaaaat
      121 agcatgaggt ttatcctcaa ttgctatgtt ttttagtaca aaaaagaggg acaaaactga
      181 gacacataag gcctcacaat ggcttgcaag gctttacatg ttttgaggta gtgggacgtg
      241 tgagcgcaga gatggcgcgg taatttgttg acttaaaatg tcgttctagg aacttctaag
      301 tcgtgggccg caggttcgaa tcctgcaggg cgcgccattt cttcttcatt tatgcccgtc
      361 ttatccgttt ccgctttgcc cttcaccaca tcactttttg tcgctgtttg gcgagataat
      421 tcaacggtgt caggccgtaa aacgccttaa aaacagagat aaagtacgac gtgctgctat
      481 agccacataa ttgcgccacc tgagtgatat ttttgttatc catcaataac atctgtacgg
      541 cgtaacgcat acgacactct gtgacaatct ggctatagct ggtattttcg ttttttaatt
      601 tctttttgag taggctgggg cttaaacata gcgaactggc gacaattcgc agattccagt
      661 aatgctgaat atcgctttga ataatgcggc agacgctgtc gcggacgctg ctgcgtaaga
      721 tatacatcag tagggctata aaccgcgatt gctcaagaaa gttagacaat acggtaaaaa
      781 gtaatgcgcg cgtcaactcc gcttcgcagg gtttgctggg ttgctgcttg ctgtggttgg
      841 cggctagccg gaacacttcc ggtgtacggc tatgacaagc gataaccggg gttgccagcc
      901 gcgaccaggg ctttacacag gttaagttct tatttaaaaa caacagataa tcgttgatga
      961 tgttacggtt gatgtggggg attaatacgt gatttaacgt tgagagttca atgacgttat
     1021 tgttgcacgc gaaggcggcc aggtagtttt tcggaagggt aaacgtagca tggttattga
     1081 cgcttacctc tacctctttt tcggtcagga tcaccacgca aggttcactg ctgctcaatt
     1141 gcatttcgca ctcctcagat atcagaaact ccgctcaaag gatctatgct tcctgcatga
     1201 gtgatcggcc cgttcgccga taacgatctt ctttctttag cacgcttttt agcaattaat
     1261 cttgatggaa ttctgacgag agcgaaagag gtaagccagg tcgtacccga cttacctgga
     1321 ggagatttaa tactcgagaa tgccgtgcgc gacgggcaaa tagcgcagag agaaatagag
     1381 agtgtcttta ccgcaagagg aacgtttcgc taacagcgag gcgatctctg catccggttt
     1441 cggtttcgct gtaaaggttt tgcctttgcc ggcgacaaac agttcataaa caatgccgct
     1501 ggcaggggcg ggactttggc aatcggcgct ggcctgtaga taacgttcac gctgttcagc
     1561 ggtaagttta tccacgccgc tgccatcctg tacccagaca ttaacgccgg ttgctttaac
     1621 ttgttccagc agttggcgat agccatcggg cgacatgttt ccggcgaaaa aactactgat
     1681 ataaaccggt tttgctgaaa catcgctaat cagccgctgc gcgttgttta accatgttag
     1741 caaaggctga cgggcagctt cgctgcgcca gttcaggtcg tcaatttccg cgctgatgta
     1801 ccagccatcc ggcgttacgc caggcgcggc gctccataat ctggcttgct ggagatcggc
     1861 agccagcagg cgattaagat agctttccag cgctgcggac gactgtttct ggtgcataaa
     1921 aaattccgga tcggcgttca gcccgacaat cagcttcagg ccagcctgtt gcgcagctgc
     1981 ggcacgctta aacaataacg cgcgctgttc tggctgggtg aatgcatcgc cgtaacgggt
     2041 ccattgcaaa accagggtat cgaagccttg caaacgtaac tgactcatca gcccctgcca
     2101 ctgggtatcg gtaacctgac tatctcggtt ttgtggttgc cagataatac ctttcatcgc
     2161 aaaggaaaaa gggctgacca aaagcagtgt cagcaatacg aaaatgaact tacgcattta
     2221 ccagtgcact ccaatggtga gaaacgcgtt gttgcgctct ccgttacgtt gattaatcgc
     2281 cttaaaggta tgttgatact cgacgccgag actgactttg tgcggccagg cgtcgtagtg
     2341 cgtctcgccg gtccagatat tccagcggac cccgactccg ccaagctgcg cgccctgagt
     2401 gcctttatca cgatagccgt tgtcctgaac gtgagcgtaa ggctcaatag tctgtccgtt
     2461 agctaccttc tgatgccagc tgacgcgata atctgccgtc cacgcctgaa tatcctggcg
     2521 gatatattgc gccgcatcga ggtacaggtt ttgggcaaac cagcctgaac cgttcgggtg
     2581 ccattcgtcg ctgtatttgc cgccattaaa gaatgaggcg ctggcgcgca gcatggtatc
     2641 ggatgcgcca ttttggccgt tcagcggcaa ctgctgttcg acggcgagga aaaagatctg
     2701 atcgcgcagc ggcttccagc gcagaccggt gccggacatc ggatttttca ccggcatcat
     2761 caccccgttt tctccggtat cggcaaagac gcggctataa actgagagca ggtcgccttc
     2821 cagcagcata ttgcgtccga tgcggtactc ggcttccagt tgcccgtagc tacgatagct
     2881 tttccctggc gctgcgccgc cgacattatt gttagcggtg ctcattgccc cggaacgcaa
     2941 gccgatagaa gaatcgaaac tgaacgtcca gcggcgaccg acctcctcat gcaaacggcg
     3001 gaaattgaag cgttgctgat tttgctctgg ggtcagtggg gttatcagcg cctgattatc
     3061 aatgtcatca atcaccagcc gggcgtagtg ctgcgtcgca ggcatgtcat ccagacgctg
     3121 gttcacgtag gccagttgtc ggatcagtgc cggatcgtcc ggaagccctt tatgcgcctg
     3181 ttcgagcatt tcccgcgact gtgcgatatc accgctatcc cacaaggcgt aaccaagcgc
     3241 tgcctggatg ttgctattat tcggttccag ttccagcgcg gcgcgcaaat cactcaccgc
     3301 cgccgggaca ttatgacgtt ggcgataaat tgtcgcccgc gcaacgtaag cgttggcaga
     3361 aggcgcaata ttgattgagc gcgtgagatc gctcagtgcg agttccggct gaccaggaat
     3421 gtaacgttgc gcatgcagcc accagtagag ggcattgttt cccagtccac gttgttctgc
     3481 ctgttgtagc cagcgatcgc gagctgcacc atttcctgcc gcctgggcgg tattggcagc
     3541 agcaagcaga tcctcattgc tcatgtcgtg aagactgatt ttctgccagg ccgccagtgc
     3601 ggtggcgtag tcctcaacct gatacgcctg ataggctacc gcacgatgtt gccaggcgtt
     3661 cggttgtcgt tgttcggcct gaagccatgc atacaaggcc acaccgggta gcgtgtcccg
     3721 ataacacttt gccagacggt tccaggcggc ggcatcgtag gaaggcgaca tatcgcccag
     3781 caagcgaact attgccgggc aattatctgc aatacccggc aactggcttt gccactgacg
     3841 ttgcgccgcc agcggcaagg gttttgataa aatcgccacc ttcgctggag ttgccaggta
     3901 aggatgactt tccagcagag acgccagtcg cgccattaaa gtctggctga cacgcgcatc
     3961 gccctggaaa ggatagcgtt gcagcaataa atcggcagct tcgcgtgact gctcgttctg
     4021 catcagttgc caggtcagtt gatctaggcg ggtaagattt gccggttctt gctgatacag
     4081 caatcgtgcc agacgcagag attcagcctt gttacgggtc gccacgctga cagcataacg
     4141 ctcctcaagc atttcattgg cggggagggt ggcgagcagt ttttgcgctg cgtcgtactg
     4201 accttctttt aacagcaccg gtagcgtcgc gccaacaaca tactggcggt tatcggcaaa
     4261 ctgcaccgta taattcgcca atgcctgaac ggggttggcg ctgtatttag ataacagata
     4321 gagccaactt ttctcttgtg cgtccgtggt aaacagcggc ttattttcaa tgagataatg
     4381 ctggaggcgt gctttttcgc cacgataagc cagcgcggtc gcgtaagtaa tatatgactg
     4441 aggatcggtg aagatcccct gtgattgcag tgccaggatc cgatcgtcca gctgcccggc
     4501 aagaagcacg tcaaaccact gacggcgttc tgccgcgctt aatgtgttct gctggcgtgc
     4561 ttcattgtat agcgtatctg cctgggacca ttgtttcagg tagattgccc gttgcagcag
     4621 atcggttcgc agcgtttttc cttccggcga tgcagcaaac gtcgcatcgt tcagttgcgc
     4681 tctggcgaca ggtaactgtg ccagccgcag ggcattctgc ccgacttcac tgcgacaacg
     4741 cagggtcggc gcagcatcgc acgctttttg ctgggcaagc agttcttcaa cagtcgtaac
     4801 gcttttcact tcaactggaa tagccgccag actgcgctca agtcgggcat ctcctgggtg
     4861 acgtttcagt tgatcctcca gcaacagccg cgcccggtca tcatgaccaa aatggcgata
     4921 ggcttccgca aggtataaag tcagcggaat attatccggc acctgctggt gtatatattc
     4981 aaattcgcgg atggcggttg cttcgtcgtt atttttctgt gccttcagcg ccttatcgag
     5041 acggggataa ataacaaaat ggcgataatc gctcagcccc agctcttctg cgctggtgcc
     5101 gatattgtct gcgagtgcgc tggtactcaa taaagacgtc agcagtaaac cagaccatcc
     5161 gatgacgcga ttaaggttat tctccttcat tttcggactc cagttgcgca acctgttctg
     5221 tgtttaaacc tgctttgagt aatagtgatt gcatcgaaac ttgtaattcg cgttgaattg
     5281 tcaggacgcg atccaacgtt tcctggctga taacgccttc ggtgaccaaa aacttgccga
     5341 gcggcagaga actgcgttca tggcgcaata acaacacgtt aattgctgaa cgattaatat
     5401 gaccgagcgt ggtcagtatt tcggcgaaca ggaactgatg cggcacatat tgccgccaga
     5461 tttcaccggt ctgctgttcc gtgagccact gatgctgaac cgcattgtac aacattgccc
     5521 gcggatcgtg accgcgtcgg cgtgcatacc agtgacgtaa ccccgtgaca atttgtcccc
     5581 gcagaacaat gacgtaacgc actttgcgtc cgactttacg cgtcagggcc gccagcgaaa
     5641 cagggtcaat accatcttca ctgccgacaa ttaactcatc attttccaga cgcagcggca
     5701 gtactgcata atgcagcgct acggaggctg gcatttcggc aatcagcgag gacgggatct
     5761 gccaggcatc gatggattcc cacgccacgc cgttttgctc tgccagcgcc tgtgccagct
     5821 gctcggcgct aatcagcccc tgcatcagca ttgaaccgcc caggcgtaga ccttcgacgc
     5881 gattacgcag tgctgtatcg agttgttctt cagtgatgac ctgattttcc agcagaattt
     5941 ggcctaacgg gcgcaacgag cgggtatcgc cagtcacgct ggggaagtca tgcgttgttt
     6001 tatcccacgc gacgcgacgt ggatcgccgt gttgaagtac ctgttttagc gcgcgccagt
     6061 tggccatgaa gttaatcagg ttgccccaga aaagacgcag gacagatagc agcccctgcg
     6121 tcagaccgta gtagccagtg acgaaaatca cccgctgcac gatgcggtta aacatcaagc
     6181 caaagtttag ccacagcagg gtcattaacc atgcgctgcc gctaaaaata gaaaggaaat
     6241 gccaggcatc gggccacaaa ctttcatacg ccagcaacag caaaagctgg agcatcacca
     6301 gcatcgcgag gaagctgaca aagttactga ttgccccttt gcggtcgcgc cagagaaagt
     6361 agttcagcgt caggctggag gtccatttgt gggttttaaa gccctggaaa acaatgccga
     6421 tgatccagcg agatttttgt cgaactgcag tcgaaaaggt atcggggaaa tattcgcgca
     6481 cgcagatcat gtttgacgtc cgtgcgtgct gtaaaaattt acgctgctcg cgttctttgg
     6541 cttcgtccac caccggaaaa cggacaaaaa tttccgtcat acctttctct ttcaggcgga
     6601 aaccaatgtc gtaatcttca gtcagactct gcacgtcgaa agcaataccg tcaccgtcag
     6661 ctaacagagc ggtcacggcg cggcggctga aacaggtgcc gacgcctgcg ctgggcactt
     6721 gtccggcgag ggcttcacgc accggaacat ctttgccatg cagctctgaa aactcatcaa
     6781 tgtaagtcat gctggtgaag tgcgtccatt cgcgttcgaa cggatacacc gggatctgaa
     6841 tcagatcttt acgctcgacc agatagttga acagacgcaa ttccatcggt gaaatcacat
     6901 cttcggcgtc atgcagaata aaaccagcaa aagcgaaatt ggcgctacgt tcaaactggg
     6961 tgatggcgtc cagcacgttg ttcagacagt cggctttgct ggtgggacca ggacgcgcgc
     7021 agactacctt atgcacgttc ggaaagcgag cgcacacttc gtcaacatca cgttgagtat
     7081 cggggtcgtt ggggtaggtg ccaacaaaga tatgatagtt ttcgtagtcg agcgtggtcg
     7141 ccgccagctc ggccatattg ccgatgacgc ccgtttcatt ccacgccgga accataatcg
     7201 ctaacggttt ttcatctggt ttatacagtt cgcggtaact cattcgcggg tagcggcgat
     7261 aaacactcaa cttgcgttta atgcggcgta cccagtagac gacatcaata aaaaaatcgt
     7321 ccagcccgct gatgaacatg atgaccgcta acgttatcgc gattactttt aagccgtaga
     7381 gccaggttgc aaaaacatca agaagccagt ccacacaaaa accttacatt aacgctggtt
     7441 atgtttaggg tggcgtatat taaggttttt tatgaattgt gacagctttt taccattaat
     7501 aggtatgact attgcggcac gttattttta cactggttat aaaagttgcc gtttgcgtag
     7561 aatgtagaca aaagcatggt gtcaaggagg ggaggttaac gtcatagtga aaaggagcgg
     7621 tggcaccgag caggtcataa tgtagtggaa gtgcctggag gaggtgagca ggcagggcaa
     7681 gattacgaaa gcccgctccc cgcaaggact gacgccagat agtttctgtc catggctgct
     7741 tttcgcatct tacgtcttaa ccctgccttg aataccttat cattcgttaa aatattaata
     7801 gcgatgtgcc gtatccctga aaataattct gcggcgtttc ctcttcttat tttgcagtcg
     7861 tcttcattca ttaccacgtc cagacgccag tgcagcttat tctccacgtg ccagtggttt
     7921 cggattgctg tggcgaactt ctctgcggtt aaatcagcag aactgatata atatctgacc
     7981 gtcatttcgg gctctttctt ttgttctgct attattgacc gaaaggagac tgccacgcat
     8041 aatttcttca gacctttcca ttcaaacgtg aaatcaataa gttcatcagg gacatcgcaa
     8101 acaatatgaa gacggatttc ttctctgccg tgactctttt cactgattgc gtaactgtca
     8161 tgctctggat tatttaattc tttcagcgga aattttccct caaaggcttt atttagcc
//
LOCUS       DAZGLJ010000192         8175 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000192 DAZGLJ010000000
VERSION     DAZGLJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.192"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(20..976)
                     /locus_tag="ACHK49_003861"
     CDS             complement(20..976)
                     /locus_tag="ACHK49_003861"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO9264423.1"
                     /translation="MFSALDIKTLMQGTLYGDPSLRVDTIRPIHSPLVGGLSIVMTPG
                     DLLHIPTTGADIIIGPEEIISSNAKAKIVVDYLNVNNLNKVLRYFKVHKYRLFEQENT
                     STIPDVYIGKHCQIGMNCHFMPGVKIMNCVTIGDNVAIHANTVIKEGTIIGNDVIIDS
                     NNSIGNYSFEYMSDERDSYVRVDSIGRVIIGDDVEIGCNNTIDRGTLGDTIIGQGTRI
                     DNQVQIGHDCIIGNKCLIVSQCGFSGHVVLGDHVITHGQVGIAGHISIGSYSVIKAKS
                     GVSHSCPEKSDLFGYPAKNTREYNKNLAVLNKLTKQHGVYKQ"
     gene            complement(995..2032)
                     /locus_tag="ACHK49_003862"
     CDS             complement(995..2032)
                     /locus_tag="ACHK49_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO9264424.1"
                     /translation="MVLIKKNVLSASIATVLLGSTGAFAANDRGFTSDTDWSEEQAGF
                     NGHVGTTFEYETKSTDNYLGKTVKEYTKTHEIFQAYFSQPNWQFAAIYGLKSIDRRQE
                     EKGYHENETSLQNYISLNKTFTIGNGFDFSLVYDLMYTDGNIDSPYVTGLHTVTVDNS
                     FRPTLTYFNSDWNAGFYSNVEYLYSRNDKSSWGVREEEGQSILFQPYKRFGAWEFGIE
                     FFYQKKHNDEFNHGKINDRSIYTETYYEPIIKYAFENAGNLYLRTRFGHGKTENADSL
                     QYNANRTYFKTIRKATLGYEQKIGDDWIAKIEYKHAWEKEHKNFYDPRDEEKYNVLNQ
                     DNIYVHAFYRF"
     gene            2305..3537
                     /locus_tag="ACHK49_003863"
     CDS             2305..3537
                     /locus_tag="ACHK49_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9264425.1"
                     /translation="MRLSNIVTVIILAISSTLTPQAMAEKLIPETDAELLIWSDATSA
                     EYMKYAATEFNKDFGYKVKFTFRNIAPMDAASRIMQDGGTTRVADVAEIEHDTLGRLV
                     VAGGVMENMVSAERIKKTFIPGAVSAATYNNISYGFPVSFATLALFYNKDLLNTAPKT
                     FEEINTFSEKFNNSSEHKYALLWDVQNYYVSRMFITLYGANEFGKTGNDPKALGIASS
                     EAKKGLETMKRLKKANPSNPLDMGNPQVLRGLFNEGKVAAVIDGPWSIQGYIDSGINF
                     GVTRIPTLDGHQPRTFSTVRLAVVSSFTEYPHAAELFADYLTTDKMLMKRYEMTNLIP
                     PIDSLMNKISQTGSEAIKAIIAQANYSDAMPSIPEMSYLWSPMTNAILATWVENKTPD
                     EVLNHAQTIIEEQLSLQE"
     gene            3540..4829
                     /locus_tag="ACHK49_003864"
     CDS             3540..4829
                     /locus_tag="ACHK49_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO9264426.1"
                     /translation="MNTQQLKMKSAPVLPISCLIMGGTQLSRHYYVKGGIFFAIQVCF
                     LLYLSDIVHTLIGLFTLGDVAQIRKGLTVIQGDNSIFMLVEGVIATIIVGLFATIYIL
                     NIKDARNSSYCHLTFKQQLYKLYEDKFAFIVLTPAFLASIAFIVLPIVITVLVSFTNY
                     AAPNHIPPKNLVDWVGIKNFIMLFKFKIWSDTFLGVALWTFIWAICATIFTFSFGFIL
                     ALALAKKNIRFAKVWRLIFILPYAIPTFVTLLIFRLLLNGIGPVNSFLNSIGMNSVDF
                     LSVPLNAKITVIAVCVWVGAPYFMLLIAGALTNISRDLYEASEVDGATKWQQFKEITL
                     PMVLHQVAPTLVMTFAYNFNNFGAIYLLTEGGPTNPEYRFAGHTDIFITWIYKLTLNF
                     QQYHIASVISIIIFIFLSVIAIWQFRRMKSFKDDVGM"
     gene            4832..5665
                     /locus_tag="ACHK49_003865"
     CDS             4832..5665
                     /locus_tag="ACHK49_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFO9264427.1"
                     /translation="MDKFLRKISTLLVYLFLICNSILVLGPVIWTIMASFKKGNNLFS
                     STFSGIEFTFDHYITLFTDTPYMQWYLNTFILATANMLISLFVVTMTAYVFSRYRFRG
                     KRNTLMSILVLQMFPAFLSMTAIYILLSKANLIDTYTGLLLVYVTGSLPFMTWLVKGY
                     FDAIPTSLDEAAKIDGAGHLTIFIQIILPLAKPILVFVGLVLFTGPWMDFILPTLVLR
                     SEDKMTLAIGIFSWIASNSAENYTLFAAGALLVAVPITLLFVFTQKHITTGLVSGAVK
                     E"
     gene            5685..7484
                     /locus_tag="ACHK49_003866"
     CDS             5685..7484
                     /locus_tag="ACHK49_003866"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFO9264428.1"
                     /translation="MITVSSLKHSAKSEDSYAYDNETLHVRLRTLRGEVDKVILWIGD
                     PYNWAEGGLDGGNMAGTEAFGWIGGNEIEMEQEAVTEFHDHWFAVFKPQKRRCRYGFI
                     LFGKEGEKFLFGEKRCVDISSPDCEERELSRLNNFFCFPYLNKIDVLNTPSWVKNTVW
                     YQIFPDRFCNCRPEISPEGVEPWGSAPTSFNFMGGDLWGVIDKLDYLEDLGINGIYFC
                     PIFTSASNHKYDTIDHFSVDPHLGGNIAFHTLIEEAHKRGIKIMLDAVFNHLGADSLI
                     WLDVVRNGANSRYADWFWIHKFPVYPDTPKSEWDFKNFNYETFGNVIEMPKLNTENEE
                     CREYLLSIVRYWTQNFDIDGWRLDVANEVDHHFWRDFRKVIKDIKPECYILGEIWHEG
                     TPWLRGDQFDSLMNYPLTYGIIDYFALQDTTKQEFMTSVTRSYLCYPKNITEVMFNLL
                     DSHDTARILSVCLNDKRKVKLAYLFMLTQAGSPCIYYGSEIGIDGFKSMTLENNRKCM
                     IWDENKQDLELRQFIRWLIRLRKKHPQWCVASIQWKDVEHPTVIAYQRDNITFFLNNS
                     EDTANFIYDGRSMEISGFSYEIEGLPAADLYDF"
     gene            7506..>8175
                     /locus_tag="ACHK49_003867"
     CDS             7506..>8175
                     /locus_tag="ACHK49_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO9264429.1"
                     /translation="MIIDAHTHIGDFVKIRMPEDVFLASLDKYNIDFALCSCGSAVEV
                     DHDQNPIPDEDQVTQHDNNERMLRLVRQHSKRIGAFMWIKPRLESCDQDFEDMIASNR
                     DIIYGIKVHPYHSKMAFNSDKVQEYIRLAQKYDLPVVSHTSNDYESSPQLVYEMALKY
                     PNVNFVMCHMGLATDNQEAIELIAKLPNLYGDTAWVRADMVYQSIKICGSDKILFGTD
                     NPING"
BASE COUNT         2384 a         1487 c         1625 g         2679 t
ORIGIN      
        1 aataatactt ctcaacgact cactgtttat atacaccatg ctgtttagtt agtttattaa
       61 ggacagcgag atttttgttg tattctcgcg tatttttggc agggtaacca aaaaggtcac
      121 tcttttcagg acaagaatgg cttacacctg atttagcctt aattaccgaa taactaccaa
      181 ttgatatgtg accggcgatt ccgacttgac catgtgtaat aacatggtcg cctaaaacta
      241 cgtgtcctga aaaaccgcat tgtgaaacaa tcaggcattt attaccgatt atgcagtcat
      301 gtccaatctg cacttgatta tcaatacgag tgccttggcc tataatcgta tctccaagtg
      361 tccctcggtc aatggtgttg ttacaaccga tttcaacatc atcacctatg attactctac
      421 cgatgctatc aactcggaca tatgaatcac gttcatccga catatattca aaactataat
      481 taccgatgga gttattagaa tctatgataa cgtcattgcc aataatagta ccttctttaa
      541 tcactgtatt cgcatgaatg gcaacattat cacctatggt cacacagttc ataattttaa
      601 caccaggcat aaaatggcag ttcataccta tctggcagtg tttaccaatg tatacatcag
      661 gtatcgttga agtgttttct tgctcaaaca aacgatactt atgtacctta aaatatctta
      721 aaactttgtt aagattattc acgttcaggt aatcgactac aatctttgcc ttagcattag
      781 aactaatgat ctcttccggt ccgataatta tatccgcacc agtcgttggt atatgcaaca
      841 aatctccagg tgtcataaca atcgagagtc caccaactaa aggactatgg attggccgaa
      901 tggtatcaac cctgagtgat ggatcaccat ataaagttcc ttgcatcaac gttttaatat
      961 ctagtgcgct gaacataggt ttccctctgt ttaatcaaaa gcgatagaaa gcatgaacat
     1021 aaatattatc ctgattgagc acattatatt tttcttcatc tcttggatca taaaagtttt
     1081 tgtgctcttt ttcccaggca tgtttatatt caatcttggc tatccagtcg tcacctattt
     1141 tttgttcata acctagagtg gcttttcgaa ttgttttgaa ataggttctg tttgcattgt
     1201 attgcaaact atctgcgttt tctgttttcc cgtgtccgaa gcgggtgcgt agatacagat
     1261 tacctgcgtt ttcaaaagca tatttgataa ttggctcgta gtaagtttcg gtataaatac
     1321 tacgatcatt tattttaccg tgattaaatt catcgttatg ctttttctgg tagaaaaatt
     1381 caataccaaa ctcccatgca ccaaaacgtt tatagggctg aaatagtata ctttgacctt
     1441 cttcttcgcg tacgccccag gagctcttat catttcgact gtataagtat tcaacgtttg
     1501 aataaaaacc tgcgttccag tcagagttaa agtacgtcaa cgtagggcga aaactgttgt
     1561 caacagttac agtatgcaag cctgtaacat aagggctatc tatatttccg tcggtataca
     1621 tcaggtcgta taccagactg aagtcgaaac cgttaccaat agtgaacgtt ttgtttagtg
     1681 atatatagtt ttgcagactt gtttcatttt cgtgatagcc tttttcttct tgccggcgat
     1741 cgatagactt aaggccatag attgctgcaa attgccaatt aggttggctg aaataggcct
     1801 ggaaaatctc gtgtgtttta gtatattcct ttactgtttt acctaaatag ttatcagtgc
     1861 ttttggtctc atattcaaat gtagtaccaa catgtccatt aaagcccgct tgttcttctg
     1921 accagtcagt gtcggaggta aagccgcgat cgtttgccgc aaaggctcct gttgagccta
     1981 aaaggactgt tgcaattgaa gccgataaga cattcttttt tattaaaacc ataaatgagt
     2041 tccttccaaa atatcaattt aagatatgat ttctgttatt ccatatagtc gtacctcatg
     2101 tactcctaaa cactctgcgg caataaaata tttttttcct caacaaatta ctgcaaatga
     2161 gatatgcatc actatttgca gttattcatc attaaaatct tatttgacag ttgattggcc
     2221 ttgatttgtg ttgataatgg cgtgaatgct tatgtaggcg tacagcatgt actacttaaa
     2281 gatattattc acgaggtaaa tataatgaga ctttcaaata tcgtcacagt tataattctt
     2341 gcaatttcca gtacgctaac accccaggcg atggcagaaa aattgattcc tgaaactgat
     2401 gctgagttat taatctggtc tgatgccaca agcgcggaat atatgaaata tgcagcaacg
     2461 gaattcaata aagattttgg ctacaaggtc aagtttacat ttcgcaatat agcgccaatg
     2521 gatgcagcat caagaattat gcaggatggg ggtacgactc gtgtagctga tgtagctgaa
     2581 attgaacatg ataccctggg gcggttagtc gttgctggcg gggttatgga aaacatggtc
     2641 tcagctgagc ggattaaaaa aacatttatt ccaggcgcag tatcggcagc tacatataat
     2701 aacatcagct atggttttcc tgtaagtttc gcaacgctgg cgcttttcta taataaggat
     2761 ttgttaaaca ccgcaccaaa aacattcgaa gaaatcaata ctttcagtga aaagtttaat
     2821 aattcatccg agcataaata tgctctgcta tgggatgtac aaaattatta tgtttcacgt
     2881 atgtttatta ccttgtatgg tgccaacgaa ttcggtaaaa ccggtaacga tcctaaagct
     2941 ctaggcatcg cttcatctga agcgaagaaa gggctagaga caatgaaacg cttaaagaaa
     3001 gcgaatcctt ctaatcctct tgatatgggt aatccacaag ttctaagagg tctgtttaat
     3061 gaaggaaaag ttgctgctgt aatcgacgga ccttggtcca tacaaggtta cattgacagc
     3121 ggaatcaatt ttggcgtgac acgcatccca acattagatg gtcatcagcc tcgcacattt
     3181 tcaacagtac gactggccgt tgtaagctca tttaccgaat atcctcatgc cgctgaatta
     3241 ttcgctgact acctgacaac ggataagatg ttgatgaagc gttatgaaat gacgaatctc
     3301 atcccaccaa tcgattcact tatgaacaaa attagccaga caggtagtga agctataaaa
     3361 gcaattattg ctcaagctaa ttattctgac gcaatgccat caataccgga aatgtcttat
     3421 ctatggtctc caatgactaa tgctattttg gctacctggg ttgagaataa aacaccggat
     3481 gaagttttaa atcatgcaca aacaattatt gaagaacaac tttcgcttca ggagtaattg
     3541 tgaatacaca acaactgaaa atgaagagtg cacctgtgct acctatttcc tgtttgatta
     3601 tgggcgggac tcagttatca cgtcattatt atgtgaaagg cgggatattc tttgcaatac
     3661 aagtatgctt tttgctttat ctttccgata ttgtgcatac attaataggt ttgttcacat
     3721 taggtgatgt cgcgcaaatt cgaaaagggt tgactgttat tcagggtgac aattctatat
     3781 ttatgcttgt agagggcgtg atagcgacca ttattgttgg cttattcgcc actatttaca
     3841 tccttaatat taaagatgcg cggaactcat cttattgcca tctgactttt aaacaacagt
     3901 tatataagct ctatgaggat aaatttgcat ttatagtcct aacgcccgca tttttagcga
     3961 gtatagcatt cattgtttta cctattgtaa ttacagttct tgtatcgttt acaaactatg
     4021 ctgcgccaaa tcacatccca ccaaagaacc tggttgattg ggttgggatt aaaaatttca
     4081 ttatgttgtt taaatttaaa atatggtctg atacgtttct aggtgtcgct ctctggacat
     4141 ttatttgggc aatttgcgcg acgattttta ccttcagttt tggctttatt ctggctttag
     4201 cactggctaa aaaaaatata cgtttcgcaa aagtctggcg attgatattt atactgccat
     4261 atgccatacc cacatttgta acgttactta ttttccgtct tcttttaaat ggaattggtc
     4321 cggttaactc gtttctgaac agtatcggaa tgaattcagt tgattttctg tcggttccat
     4381 taaatgcaaa aataacagta attgccgttt gtgtttgggt aggggcaccg tattttatgc
     4441 tattgatcgc gggtgcatta acaaacatat cccgagattt gtatgaggct agtgaggtcg
     4501 atggcgcaac taaatggcaa cagtttaagg aaattacatt gccaatggtg ttacatcagg
     4561 ttgcgccaac tctggtaatg acatttgcat ataatttcaa taattttggt gcaatttatc
     4621 ttctgactga aggtggtccg actaatccgg aatacagatt cgctgggcat accgatattt
     4681 ttattacctg gatttataaa ctcacattga atttccagca atatcatata gcttctgtaa
     4741 tctccatcat aatatttatc ttcttatcgg ttatagcaat atggcaattc agacggatga
     4801 aatcatttaa agatgatgta gggatgtgac aatggataaa tttttacgta aaatatcaac
     4861 gctcttagtt tatctttttt taatctgcaa ctccattctt gtgcttgggc cagtgatatg
     4921 gacgataatg gcatctttta aaaaagggaa taatctattt agttcaacat ttagtggcat
     4981 cgagtttaca tttgaccact acataacgtt gttcacggat acgccatata tgcagtggta
     5041 tttgaacact ttcattcttg ctaccgctaa catgttaatt tcactgttcg ttgtgacgat
     5101 gaccgcctac gttttttcaa ggtatcgatt tcgtggaaag cgtaatactt taatgagtat
     5161 tcttgtattg caaatgtttc ctgcattttt atcaatgact gcaatttata tcctgctatc
     5221 aaaggccaat ttgatagaca catatactgg gttgcttctg gtttatgtaa ccggttcatt
     5281 gcccttcatg acgtggttgg taaaaggtta ttttgatgct attccaactt ctttggatga
     5341 ggccgctaaa atagatggag cagggcatct gactattttt attcagataa tattgccatt
     5401 ggctaagccg attctcgttt tcgttggatt agttttgttc actggaccat ggatggactt
     5461 catcttgcca acgttggtgc ttcgtagtga ggataaaatg accctggcga ttggaatttt
     5521 tagttggatt gcttctaatt cagcagagaa ttatacgctt tttgctgctg gggcgctttt
     5581 agttgcagta cctatcacat tgttatttgt attcacacag aagcatataa ctactggact
     5641 tgttagcggt gccgttaagg aataattttt agggattagt atttatgatt acagttagtt
     5701 cgttaaaaca ctcagcaaaa agtgaagata gctatgccta tgataacgaa actttgcatg
     5761 tacggttacg cacattaaga ggagaagttg ataaagttat tctgtggata ggcgatccat
     5821 ataattgggc tgaaggtggt cttgacggcg gtaacatggc cgggacagaa gcgttcggct
     5881 ggattggcgg caacgagatc gagatggaac aagaagctgt caccgaattt catgatcact
     5941 ggtttgcagt gttcaaacct caaaaacgcc gttgccgtta cggttttata ctttttggga
     6001 aagagggaga gaagtttctt tttggtgaga aaaggtgtgt tgatatcagt tcaccggact
     6061 gcgaagaacg agagttaagc cgcttaaata actttttttg cttcccttat cttaataaaa
     6121 ttgacgttct aaataccccc tcctgggtaa aaaatacggt atggtaccaa atttttccag
     6181 accgtttctg taactgcaga cctgaaatat ccccagaagg ggtagaaccg tggggcagtg
     6241 caccgacttc attcaacttt atgggtgggg atctgtgggg ggttattgat aaactcgatt
     6301 atctggaaga tctgggtatt aatgggattt atttttgtcc tatttttacc tcagcttcca
     6361 accataaata tgacaccatc gatcatttta gtgttgatcc acatttaggt gggaatattg
     6421 ctttccatac tttgattgaa gaagcgcata agcgtggaat aaaaatcatg ttggatgcag
     6481 tattcaatca tttaggcgct gactctctaa tatggcttga cgttgtacgc aatggtgcca
     6541 attcacgtta cgccgattgg ttctggattc ataaatttcc tgtttatccc gatactccaa
     6601 aaagtgaatg ggattttaaa aactttaact atgaaacctt tggcaatgta attgaaatgc
     6661 ccaagctaaa tactgagaat gaagagtgcc gtgaatactt acttagtata gttcgctatt
     6721 ggacgcaaaa ttttgatatt gatggttggc gtctggatgt tgcgaatgaa gtggatcatc
     6781 atttctggcg agatttcaga aaggtgatta aagatatcaa acctgaatgt tatattttgg
     6841 gtgagatatg gcatgagggc accccctggt tgcgtggtga tcagtttgat tcattaatga
     6901 attatccctt aacatatggg attattgatt attttgcgtt acaggataca acaaaacaag
     6961 aatttatgac atccgttaca cgttcttatc tttgttatcc taaaaatata actgaagtta
     7021 tgtttaatct tctggatagt catgacaccg cgcgtatttt aagcgtttgt ttgaatgata
     7081 aacgaaaagt taaactcgcc tatttattta tgttaactca agcgggctct ccttgtatct
     7141 actacggtag cgaaatagga attgatggtt ttaaatcaat gacgcttgag aataatagaa
     7201 aatgtatgat atgggatgaa aacaaacaag atcttgaatt acggcaattt atacgttggt
     7261 taatacgtct acgtaaaaaa catccacaat ggtgtgtggc atcaatacaa tggaaagatg
     7321 ttgaacatcc tacggtgatc gcttatcaac gcgataacat cactttcttc ttaaataata
     7381 gtgaagatac tgccaacttt atttatgacg gaagaagtat ggagatctcc ggtttcagtt
     7441 atgaaattga aggtttacct gctgctgatc tttatgattt ttaaatttca attaggatgt
     7501 ttgttatgat aattgatgca cacactcata ttggtgactt tgtgaaaatc cgtatgccag
     7561 aagatgtttt tctggcatct ctggacaagt ataatattga ttttgcatta tgttcttgtg
     7621 gatctgcagt agaagttgac catgaccaga acccgattcc ggatgaagat caagttacac
     7681 agcatgataa taatgaacgt atgctcagac ttgttcgtca acattcaaag cgaattggcg
     7741 cgtttatgtg gattaagcct cgtctggaaa gttgtgatca agattttgaa gatatgattg
     7801 ccagcaatcg agatatcatt tatggcatca aagttcaccc atatcattca aaaatggctt
     7861 tcaattcaga taaagttcag gaatatatac gccttgctca aaaatatgat ctcccggtag
     7921 ttagccatac ttctaatgat tatgaatcca gcccacagtt ggtttatgag atggcactta
     7981 aatatccaaa tgtgaatttt gttatgtgtc atatggggct cgcgacagat aatcaggaag
     8041 cgatcgaatt gattgcaaaa cttcctaatc tctatggcga tactgcgtgg gttagggcgg
     8101 atatggttta ccagtcgata aaaatctgtg gtagtgataa gattttattt ggtaccgata
     8161 atcctattaa tggac
//
LOCUS       DAZGLJ010000193         8113 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000193 DAZGLJ010000000
VERSION     DAZGLJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.193"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            124..564
                     /locus_tag="ACHK49_003868"
     CDS             124..564
                     /locus_tag="ACHK49_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HFO9264430.1"
                     /translation="MSKKKFSVENEDLATVRTSLLNSNLVRKLIMEMSDEDFNQVKDN
                     LEKAIEQRNELKDSLAIIKKYMDDAGVTAEDVYREFGKKLQTGRKNDSGESNKYRYEY
                     NGQIMYWSGQGRRPSVIAEAIKAGGSLDDFLISQGTSSSCSDYE"
     gene            832..960
                     /locus_tag="ACHK49_003869"
                     /pseudo
     CDS             832..960
                     /locus_tag="ACHK49_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(992..1621)
                     /gene="istA"
                     /locus_tag="ACHK49_003870"
                     /pseudo
     CDS             complement(992..1621)
                     /gene="istA"
                     /locus_tag="ACHK49_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
     gene            2782..3348
                     /locus_tag="ACHK49_003871"
     CDS             2782..3348
                     /locus_tag="ACHK49_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO9264431.1"
                     /translation="MPKSYTPNWFFTALLDNHINQMMARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTADHGFHAHVVFWIDRQRVKKIY
                     PFVERITECWRSITHNSGSVHRCTYQPHYTYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCNEVPERPAAGRPRKPHF"
     gene            3595..4167
                     /locus_tag="ACHK49_003872"
     CDS             3595..4167
                     /locus_tag="ACHK49_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264432.1"
                     /translation="MNAIPYFDYSLAPFWPSYQNKVIGVLESALREQSGSRIRRILLR
                     LPCEYDRTFSSRKIWFGMDFIETVSALMNATPGRDLCWLLTHHPEKPEYHAVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAKQYQKQITRDVVLDSRSPDCEERLKELIWAF
                     SDFARDRRGVHDPEARYLASNPWYPVAGQL"
     gene            4236..4469
                     /locus_tag="ACHK49_003873"
     CDS             4236..4469
                     /locus_tag="ACHK49_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9264433.1"
                     /translation="MLTTTSHDSVWLRADDPLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            4670..5218
                     /locus_tag="ACHK49_003874"
     CDS             4670..5218
                     /locus_tag="ACHK49_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264434.1"
                     /translation="MQITETVLSEPVEIRRFIQQAVDHWPRLLAVHFTLHSAEGNING
                     QQIHAFCTSFYRQVHERITERNHTASPAPPVVLRWLREQHGGATMRCLLLLSQTSICH
                     PRVSATVDEECSQRVDLLQQTWRVISADGQCRVERCFQAARGDTSGQYIALKTVALSL
                     GLPVVTGITHRPVQRCTLITAQ"
     gene            complement(5236..5744)
                     /locus_tag="ACHK49_003875"
                     /pseudo
     CDS             complement(5236..5744)
                     /locus_tag="ACHK49_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258200.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
     gene            complement(6117..6808)
                     /locus_tag="ACHK49_003876"
                     /pseudo
     CDS             complement(6117..6808)
                     /locus_tag="ACHK49_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            6868..7044
                     /locus_tag="ACHK49_003877"
                     /pseudo
     CDS             6868..7044
                     /locus_tag="ACHK49_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
     gene            7233..7408
                     /locus_tag="ACHK49_003878"
                     /pseudo
     CDS             7233..7408
                     /locus_tag="ACHK49_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2104 a         1788 c         1899 g         2322 t
ORIGIN      
        1 aaagttcact cactttgaat gaagtaaata ttaagtatca cattttgtga tgtagccatt
       61 atttttataa aaaatcacct gataggattt atcataagtt aatgataagc agagtattga
      121 tagatgagca agaagaagtt ttctgttgag aatgaagatc tcgctactgt tcgtacatca
      181 cttcttaatt ccaaccttgt ccggaagttg atcatggaaa tgtctgatga agacttcaat
      241 caagttaagg ataaccttga aaaggccatt gagcagcgta atgagttgaa ggattctttg
      301 gcaattatca aaaagtacat ggatgacgca ggtgtaacgg cggaagatgt gtatcgtgaa
      361 tttggcaaaa aattacagac gggcagaaaa aacgattcag gagagtctaa taagtacagg
      421 tatgagtata acggtcaaat aatgtactgg agtggacaag gtcgtcggcc ttctgttatt
      481 gcagaagcaa taaaagccgg aggttctctt gatgattttc ttatatctca gggcacatca
      541 tcatcttgtt ctgattatga gtgatacatc taatcggttg aatgttgctg taatgctcgc
      601 ttttgatgct tatatggcag cgttaccgtt tattgtttta gttacaaatt ggtcgtcatg
      661 gacataacct gaaattataa ctatcatcgg gtctttcaag ttgcttatgc atataacgcc
      721 agggaggaaa acgagagtta aaaaattgaa ctgttctcat agagactgta atttaaattg
      781 tgtaattgcc ggttttgata tgttcgctcc aacaacgtag acaagtagat tgtggacgaa
      841 aagacgctta aagtccttgc ggctgagctg gctaaaggcc tcaaaaccga ggccgacctc
      901 aatcagtttt cccgtatgct gacgaaatta actgtcgtaa cggcgcttaa tgcagaactc
      961 ggtaacggct gcagatgttc ctgctctggt tgtggtcggc cagcaacagg aatccggagt
     1021 tgaacacgac tttcccgttg ttattcttca gcaccgcagc cttctggtta tcaaccagca
     1081 ccgttttcac acaaccaccg aagtagcgga aggcacgaac cagtgattcg taggtatgct
     1141 cagcatcctg ttttggtgct gcgaagacat ggaagcggcg ggagaacccc agcgtattaa
     1201 ccgcaaagtt aactttgcac cgttgcccgg caacctccac ctcaacttcg ccccagtcgt
     1261 gctggagctg gtatccaggc tgagtttcga agcgaactgt tctttttgac ggacgcattt
     1321 tacgtttggg ctggatgtag taacgcagca tggaacgtcc gcccgtataa cccattgcct
     1381 taatctccgc gaagataacc tcactattcc agacattctc tgccaggcgc atgtcgatgt
     1441 aatccataaa cggtttcagc ttaaccattt tgtggcgggt ctttctggct ggcggttcag
     1501 ggtatttgag gtagcgtctg accgtccgtt cagagcaacc aatctgagtc gcaatatcga
     1561 caatgtacgc gccctgctgg cgcatttgct ttatcatgta aaagtcctct ctgctcagca
     1621 tgttgatgtc ctttctggtg tgagaacctt aaggaaacaa catgttgggt ggagcggaca
     1681 atccaaatgg tgaccgtctt attatcgctg gcgctgacac ctgagcgatc acctttaata
     1741 tggcagcact tacactgaat tatttacata ttcgttataa cgaacttctg tacataatta
     1801 tgaaacagat ttgtatttac ataaaacatg gatgttttta atttaggttt attactatta
     1861 ttatactcac tccctttttt atatgatctc tattgttatt tacttcctgt cttcactcac
     1921 tctcaatctt tgcatattcc attagtatta ctcttgttat cgtattcact ccatccttca
     1981 atcataatta ctgtaactca tatgatattc agataagtta ttctctacta cgctattgct
     2041 gatatccatc tgttcctgtt ttcagtgaaa taataattga ttttaattta tccatcatga
     2101 actgtattgg attaacaatg attgtttatc tgaagtgttt taaccattct ggtgagcctc
     2161 agttcttcag tcgtcactga ttaactgaat gtttattctt tgataaggta tccatgattc
     2221 tgtaacgatt aacagagctg gataaacaac agaatcaaca gagtgaattc ctgattatgt
     2281 ttgtttcggg ttgtattgtt taagatgcag ggtaaaaatc atgccagtgg tattttttac
     2341 tctggtaatg tgtgcggtta acgttcatgt ccttaagaac agattggatg agggcgtata
     2401 agtttattgc gttggccttg tacacgtttt ttcagactca ctgagagcaa gtttgtagtt
     2461 tatgatgtta ttggtcgtaa tatgtttctt aatctcctgg tagtgggttt ttatcgcgtg
     2521 ttttgcagta tttcgtgatg tttattgatt ctgtatttta ttcgctcctc gtttatctca
     2581 tctctttagc taataccatc agataatcca tttctttctg cataatgctg cgtatcgtta
     2641 ataactcgtc gtatccattc tgctacagca tgcctgataa ataccatctg taagttatta
     2701 ccgttttaga tctgattatg agcgaaagca ttaattcgct cacagagctt aaaacatcat
     2761 taactttcag gagtcatcaa catgcctaaa tcttacacac caaactggtt ttttaccgct
     2821 ttacttgaca atcacatcaa tcaaatgatg gcacgttatt cctgcctgcg ggccttacga
     2881 atggatttct tctacaggaa agatacgcct gatttcttac aacctgatca tcgctggctt
     2941 gaattgcagt tgcgtatgct gctggaacag gttgaacaat ttgaaaatat tgttggcttc
     3001 ttctgggtga ttgaatggac ggctgatcat ggttttcatg cgcatgtggt tttctggatc
     3061 gatcgtcaga gggttaaaaa aatatatccc tttgtggagc ggattacgga atgctggcgg
     3121 tctattacgc ataacagcgg ttcggtacat cgctgtacat atcagccgca ttatacatac
     3181 aacatcaaca ttcctgtgcg ccacaacgat cctgaaagca tcgataatat tcgcggtgcc
     3241 ctgcattatc tggcgaaaga agagcaaaaa gacgggctgt gtgcctacgg ctgcaatgaa
     3301 gttcctgaac gtcctgctgc agggcgtcct cgtaagcctc acttctgaag cttaaggcct
     3361 gagcctccgc tcctggaaac actccgtcgg taaaaactta ccgccttgat taatgatgtg
     3421 aaccgaagtc aacggagatc attccccctg aacctgcatc cagtgttctt tcccttatct
     3481 tcccgttctg cttcagttct tcacttattc catcaatctc atccgcaagc cataacacgt
     3541 cagctcattc acgggcagga cgcattgtgg gctgcgcata acggaacata tcttatgaat
     3601 gctattcctt atttcgacta tagcctggca cccttctggc catcttatca gaacaaagtc
     3661 atcggcgtcc ttgagagtgc gctgcgtgag cagtccggct cccggatacg acggatcctg
     3721 cttcgtctgc catgtgaata tgaccgtacc ttcagcagca gaaagatctg gttcggtatg
     3781 gactttatcg aaaccgtcag tgcgctgatg aatgcaacac ctggacgaga tctctgctgg
     3841 ctcctgaccc atcatcctga aaaacctgaa taccacgcgg tgctgtgcgt cagacaggag
     3901 tatttcgatg gccctgaact ggaccggttg atactggatg cctggagtaa cgtactgggc
     3961 ttcgcgtcac cgggtgaagc aaagcagtat cagaagcaga tcacccggga tgtggtgctg
     4021 gacagtcgtt caccggactg cgaagagaga ctcaaagagt tgatatgggc gttcagtgat
     4081 ttcgcccgcg atcgtcgtgg tgtgcacgat ccggaagccc gttacctcgc cagtaatccc
     4141 tggtatcccg tagcaggcca gctctgaaag ctcggttcag catctttccc tgcattcgcc
     4201 taaccttcat aaatctcact gaggacattc tgaccatgtt gaccacaaca agccacgaca
     4261 gcgtatggct gcgtgccgac gatcccctga tcgacatgaa ctacatcacc agtttcactg
     4321 gcatgaccga taaatggttt tacaagctga tcagtgaagg ccatttccct aaacccatca
     4381 aactggggcg cagcagccgc tggtacaaaa gcgaagtgga gcagtggatg caacagcgaa
     4441 tcgaggagtc acgaggagca gcagcatgaa acgtgttgta atgccggtac gctggcaatg
     4501 tgcaaaatgc cggcgctggt attgcggaaa tcagccctgt ccctggtgct ggcgacctgc
     4561 tgcccccgtc tgtcattcct cgttcttcgc tgtaacaggc atcgtaccgg tctcggcgtg
     4621 ctttcctgcg taccgacacc ggttcagcaa cattcaaggg gatcaaatcg tgcaaataac
     4681 cgaaaccgta ctttcagagc cggtggaaat ccggcgtttt attcagcagg cggtggatca
     4741 ctggccgcgt ctgctggccg tccacttcac actccattcg gcagaaggaa acatcaacgg
     4801 acaacagatt catgcattct gtacttcctt ttaccgacag gtgcacgagc gtattactga
     4861 acgtaatcac acggccagtc cagcaccacc ggtggtatta cgctggttgc gggaacaaca
     4921 tggaggagca acaatgcgat gcctgttgct gctcagccag acgagcattt gccatccacg
     4981 ggtcagtgcc acggttgatg aagaatgttc gcaacgggtg gatttattgc aacagacatg
     5041 gcgggtgata agtgctgacg gacaatgccg ggtggaaagg tgttttcagg ctgcccgggg
     5101 tgatacatcc ggccagtata ttgcgttaaa aacagttgca ttgtctctgg ggttaccggt
     5161 tgtgaccggt attacccatc gcccggtaca gcgctgtaca ttgattacag ctcagtgaac
     5221 agcgctgttg tgcagtcaac gaggttttct gtcttttcgt cggtcattca gcatcattaa
     5281 acgggtctct gcctctttct gttcctgtgg tgtcacttcc ccgcacggct gaccgttcag
     5341 gttgtaacgg gcgccacctg ctattaacgc acggtaatag cgcggagact gcacataaga
     5401 cgccactgct gcatgtaatg taccagcgcc aaacgccagt tctcggatgg cgatatcctg
     5461 tatcaggtcg tcaggtatcc ccactttaag tggttttgtt tttttcccgg ttgaatgact
     5521 caggccagag ttccacaaga cgaataatgc gcttacggtt tttgctctgg cgttttgtca
     5581 tgtcgccacg gtgttttatc agcaggtttc tgtttatcgt tcaactgtgg tatctggtca
     5641 ccggccttca ttttgtatgc tgtgtccgtt ttatcctgca gcggcgttgc cggtgattta
     5701 ctgaaaatac cctgcggttt acggttaatg gtcagagtta ccatcaattt tgttacctcg
     5761 cgctgtcaat ggaagtagta cctcatccga tatctgaaaa tgttttccgg tggatgttac
     5821 agcgaatttc atgtaagaca tgttgttgtc ggtattgttc agtactgtgc gttgtgagcg
     5881 tattcgttat cagacagagg ggcagggaac aaatggcgta aacggacatc tcagtgcctg
     5941 agaaacagta caggtcaggc catcatggcc tgcaaacaga gccctggcga ccccgtggcc
     6001 gaaaacgaaa caataacccg cgcgcagtgc gaggaatccg gatgtgaagc aatcagcagg
     6061 taatattaat ggcaggacca gcaggttctt taaacaaacg aaggtggtga ctccaattag
     6121 tagaacatat atttttcgat aaaggcaccg atgacttttt catgaagttc tacagagcgc
     6181 gagaaacaga tagttctgcg ggccagacgt ttgattcggg ttctcagagg tagatgttgc
     6241 gctcaatgcg ctgagtaaaa atcttaccag tcagatgctt ctccttcggt aactctctgg
     6301 tgtaactacc ccattcgtca gtggtcaaca taccgatggt aaaagggcaa gcagcgccag
     6361 caattcacga caggtttaat cagtccgtag gccaaaagtg taggccagaa cgccaccagt
     6421 tttcgtgttg tacgtgtacc agagccagtg ctgtcgcgct ttactgccga caaaactcca
     6481 ttgctcatca agttcgcaga tgagtgccat atcagcatga gcgaccgggt atgacgttat
     6541 tcttcgtggc gagagttttt taaagtccgg atgacagtat taatgccgat tttcagcgta
     6601 cgggcagtat cgcgaactcc ggcaccgttg aaagccattt cagtgatttg ctctttaact
     6661 cccggtttac gggcctcgta acggtaagtg agctgaagca ctcgatgaca gtcacggcag
     6721 cgaaatcgat catgtccttt gggttcttcc cgtgacggta aacctgaacg gactggcaac
     6781 aggagcaatg aaggttaact gtgaccatga aagaacctta aaagtggcta ttatacccct
     6841 aattcaacta attggagtca tgaccgtatt cataatggat taggttcagc agttgctgaa
     6901 gtgcttgtag aaacttgtcc tgtgcccatg cgtcgggtgg gggttaaaga gcggtatggc
     6961 caggttgaaa ctcaggactt tttacagaga gagtacgggt taaccgcgca agctattgtg
     7021 gcggcagcca gagagctatt gtaattcatg gagtttgttt accattctgt ttttcttaaa
     7081 agtgagtgtc tgtgcggtca ctaaactcgg taataaagca gttcattgca ggataagaag
     7141 cgtttggttg tgtcgtttta ccgtaagaac cgaccgccgt atgtatccat taagccagta
     7201 ttggtaacgt aggtgcccac cttttcaaac tggtgggcgc ctgttatgga gcaaaaacat
     7261 tatccgcaga accccgcaga tcattttcaa atgagttaaa cttcaaatgg ttaaactggc
     7321 tttgcaacca ggagcctctg ttgcccgtat tgcccgggaa cacgatatca atgataacct
     7381 cctgttcaaa tggctcagac tctggcagaa cgtaaggtaa agggagatga aaaaggcaat
     7441 agcagaactg agtgacagtg aaaccattct ggcgtacagg gttggatggt gaaactgata
     7501 aaaaaaacct ctgaaaattc tgtcagaggg tgtagtcaaa aatggtagat atttacgatg
     7561 cagtaatgct ttcactccgg taattaattt ccgtgattaa ctgctttcga tcaataaagg
     7621 aattgcaaaa gtgtgatctt gttcacgaca tcgtggatga taatatgcct gatgtctgaa
     7681 tccggaaaca gtgctctggc gggtttcatg tgaagttgac ggataaaaaa tccgcacgac
     7741 cagacagacg tgcggtttac gaaattcgta acaagaggct tttctacgga tcacgtggtg
     7801 cgtgccacgt gaaatttttg tagcgcaaaa tgtgcaatgt gtaaatacaa ggaaggaaaa
     7861 aagttaaatt ggtagaagtc catgcctgcc acatgtacgt ctgcattcct cgttttgaac
     7921 agaattctca ccagacatcg tgaatttctc attcaatcat cagagatatt aaccaggatt
     7981 tttattgttt tgtgatatca gcagtgcagg tatacagacg ttaaagtaat caggtttatt
     8041 ataaccacct ttacaaagtt gtatatgttt ataatttaat gattttaatg ccagtcgttt
     8101 tctgctgctt aaa
//
LOCUS       DAZGLJ010000194         8098 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000194 DAZGLJ010000000
VERSION     DAZGLJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.194"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            42..872
                     /locus_tag="ACHK49_003879"
     CDS             42..872
                     /locus_tag="ACHK49_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO9264435.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            904..1839
                     /locus_tag="ACHK49_003880"
     CDS             904..1839
                     /locus_tag="ACHK49_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO9264436.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            1932..2174
                     /gene="yhdT"
                     /locus_tag="ACHK49_003881"
     CDS             1932..2174
                     /gene="yhdT"
                     /locus_tag="ACHK49_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO9264437.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            2164..3615
                     /gene="panF"
                     /locus_tag="ACHK49_003882"
     CDS             2164..3615
                     /gene="panF"
                     /locus_tag="ACHK49_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO9264438.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            3627..4508
                     /gene="prmA"
                     /locus_tag="ACHK49_003883"
     CDS             3627..4508
                     /gene="prmA"
                     /locus_tag="ACHK49_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO9264439.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            4837..5802
                     /gene="dusB"
                     /locus_tag="ACHK49_003884"
     CDS             4837..5802
                     /gene="dusB"
                     /locus_tag="ACHK49_003884"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO9264440.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            5828..6124
                     /gene="fis"
                     /locus_tag="ACHK49_003885"
     CDS             5828..6124
                     /gene="fis"
                     /locus_tag="ACHK49_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO9264441.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            6210..7094
                     /gene="yhdJ"
                     /locus_tag="ACHK49_003886"
     CDS             6210..7094
                     /gene="yhdJ"
                     /locus_tag="ACHK49_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO9264442.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            7178..7357
                     /gene="yhdU"
                     /locus_tag="ACHK49_003887"
     CDS             7178..7357
                     /gene="yhdU"
                     /locus_tag="ACHK49_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO9264443.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(7360..8022)
                     /gene="acrS"
                     /locus_tag="ACHK49_003888"
     CDS             complement(7360..8022)
                     /gene="acrS"
                     /locus_tag="ACHK49_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO9264444.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT         1997 a         1963 c         2044 g         2094 t
ORIGIN      
        1 agaaaaatac aaggggctat agtgttacgc attaaaggcc aatgaagagt gttaacgtgc
       61 cgagaaaatc gtctaaccta ctgaaacgca gattaaaaat tgcagaaatt gttgccgccc
      121 aaggtgaaat caaagttgat gacttatctg cgcaacttgg tgtctctggc gtgacgatcc
      181 gcggcgatct cagttatctg gagcaacagg gatatttaaa gcgatcattt ggcggtgcta
      241 ttgcgacacc actctcctct gaaccagaag cgatacaacc gatcgcgcca ttgtctctgg
      301 cacaacaaat ggaaatagca cgacattgcg cacggttaat tcgcgatcgt gacacgctat
      361 ttcttggaca tgggacaatc tgccgtaaga tcattccatt gctcagcgaa gtgaaaaaac
      421 tgcgtctgat caccaacgat cccgaacatg cgctgctggc aaatcagttt attgatggcg
      481 aaataatact ggctggcagt gaaatgctgc gccccgacac cgcactggcc ggaaaaccgc
      541 ttgaacaggc acttcagcat ttcaccatta cacatagcat tctggaaatc agccatctgg
      601 atgctgatgg tactttaaat atcaacgttc ccagactggc cgctgcctgg cagctctgtt
      661 tcgatcatgc ccagaataag acggttatcg ttgccgccga tcctgcgcca tcaacggctg
      721 ctatcgggca tttgcatcag gcagaaaatg tgatcgtcag ccgcagcatt aacgaacaat
      781 atcagcaaca attgcagtcg gctgatttcg ttatccttta taccagtaat gagtgcttta
      841 cctggtccaa tcgcgaaagg cttcaggaat gagaggggtt ttgtgacaat aactatctgc
      901 gctatgggcg aattgctggc cgagtttttg tcccgcaacc cacatcaaaa attcactcag
      961 cctggggagt ttatcgggcc atttcccagc ggtgcgccag caatttttgc tgctcaggtg
     1021 gcaaaactgt cccatcgggc catcttcttc ggatgtgttg gtaatgatga ttttggccga
     1081 ctcattatag agcgcctccg tcatgaaggt gtcattaccg atgggatcca tgttatgaac
     1141 aatgccgtca caggtacggc gttcgtgagt tatcaaaatc cccagcagcg cgatttcgtc
     1201 tttaatatcc ctaacagcgc ctgcggcttg tttactgctg agcacattga taaggatctg
     1261 ctaaaacagt gtaaccatct ccatattgtg ggctcatcgt tgttctcatt tcgcatgatc
     1321 gatgtcatgc gtaaagcaat aacgacgatc aaatcggctg gcggcaccgt ttctttcgat
     1381 cccaatattc gcaaagagat gctgagcatt cctgaaatgg cgcaggctct cgattatttg
     1441 attgaatata cggatatttt catccccagc gaaagcgaac tccctttctt cgcgcgtcac
     1501 aaaaatctgt cagaagaaca gattgttagc gatcttctcc acggcggcgt aaaacatgtg
     1561 gcgataaaac gcgcccagcg tggggccagc tattacaagc ttaaaaacgg tacattacac
     1621 gcccagcatg ttacaggcca cgatatcgaa attatcgatc caacgggtgc aggcgactgc
     1681 tttggcgcaa cgtttatcac tcttttctta tccggtttcc cggcacataa ggcactgcaa
     1741 tatgcaaatg ccagcggcgc gctcgccgta atgcggcaag gtccgatgga agggatatcc
     1801 tcactggctg acattgaaga ctttttgcag cagcactgaa cggcaatttt ctgtcagatt
     1861 attttccctg cctgaagagt tgccgtagaa ttgcccgttt ctcgtatccc cccatgagga
     1921 ggaacccttc cgtggacact cgttttgttc aggcccataa agaggcgcgc tgggcgctgg
     1981 ggctgaccct tttgtatctg gcagtttggt tagtagccgc ttacttacct ggcgttgccc
     2041 ccggttttac cggctttccg cgctggtttg agatggcctg catcctgacg ccgctgctgt
     2101 ttattggact gtgctgggcg atggtgaaat ttatctatcg cgatatccca ctggaggatg
     2161 acgatgcagc ttgaagtaat tctaccgctg gtcgcctatc tggtggtggt gttcggtatc
     2221 tcggtttatg cgatgcgtaa acggagcacc ggcaccttcc ttaatgagta tttcctcggc
     2281 agccgctcta tgggcggtat tgtgctggcg atgacgctca ccgcgaccta tatcagtgcc
     2341 agttcgttta tcggcgggcc aggagctgct tataaatacg ggctgggctg ggtattgctg
     2401 gcgatgattc agcttcctgc agtctggctt tcactcggta ttctcggcaa gaagtttgcg
     2461 attcttgcgc gccgctacaa tgcagtgacg ctgaacgata tgctgtttgc ccgctaccag
     2521 agtcgtcttc tggtgtggct ggcgagtttg agtttgctgg ttgcgttcgt tggtgcgatg
     2581 accgtgcagt ttatcggcgg tgcgcgcctg ctggaaaccg cggcgggtat tccttatgaa
     2641 accgggctgc tgatttttgg tatcagcatt gcgttatata ccgcctttgg tggctttcgc
     2701 gccagcgtgc tgaacgacac catgcaaggg cttgtgatgc tgattggcac cgttgtgctg
     2761 cttattggcg tggtacatgc cgctggcggc ttaagcaacg cagtacagac cttgcaaacc
     2821 atcgatccgc aactggttac gccacaaggc gctgacgata ttctgtcgcc tgcctttatg
     2881 acgtcgttct gggtactggt gtgctttggc gtgattggcc tgccgcatac tgcggtgcgc
     2941 tgtatctctt ataaagacag caaagcagta caccggggga tcatcatcgg tacgattgta
     3001 gtcgcaatcc tgatgttcgg tatgcacctg gccggagcgt taggtcgtgc agtgatcccc
     3061 gatctcaccg taccggatct ggtgatcccg acgttaatgg taaaagtgct gccaccgttt
     3121 gctgccggaa tcttcctggc tgcacctatg gctgcgatca tgtcgacgat caatgcccag
     3181 ttgctgcaaa gttccgctac gatcattaaa gatctctatc tgaatatccg tccggatcaa
     3241 atgcaaaacg agacgcgtct gaagcggatg tcggcggtaa ttacgttagt tctcggcgcg
     3301 ttgctgctgc ttgccgcctg gaagccgcca gaaatgatca tctggctgaa tttgctggcc
     3361 ttcggtgggc tggaagccgt tttcctgtgg ccgctggtgc tgggtcttta ctgggaacgc
     3421 gccaacgcca aaggcgcgct aagtgcgatg atcgttggcg gcgtgctgta tgccgtactc
     3481 gcgacgctga atattcagta cctgggcttc caccctattg tgccctcgtt actactaagt
     3541 ttgctggctt tcctggtcgg aaaccgtttc ggtacatccg tcccgcaagc taccgttttg
     3601 actactgata aataaagagt tttgccatgc cttggatcca actgaaactg aacaccaccg
     3661 gcgcgaacgc ggaagatctt agcgatgcgc tgatggaagc gggtgccgtt tctatcactt
     3721 ttcaggatac ccacgatacg ccagtatttg agccgctgcc gggcgaaacg cgcctgtggg
     3781 gcgacaccga tgtgattggt ctgttcgacg ctgaaaccga tatgaacgac gtggtggcga
     3841 ttctggaaaa ccatccgctg ctcggcgcag gcttcgcgca taaaatcgaa caactagaag
     3901 ataaagactg ggagcgcgaa tggatggata atttccaccc gatgcgcttt ggtgaacgac
     3961 tgtggatctg ccctagctgg cgtgatgtgc cggacgaaaa cgccgtcaac gtgatgttag
     4021 atccagggct ggcgtttggt acgggtaccc atccaaccac ctctctgtgc ctgcaatggc
     4081 tcgacagcct cgatttaacc ggtaaaacag tcatcgactt tggctgtggt tccggcattc
     4141 tggcgatcgc ggcgctgaaa ctgggtgcag caaaagccat tggtattgat atcgatccgc
     4201 aggcgattca ggccagccgc gacaacgccg aacgtaatgg cgtttctgac cgtctggaac
     4261 tctacttacc gaaagatcag ccagaagaaa tgaaagccga cgtggtggtc gctaacatcc
     4321 ttgcaggccc attacgtgaa ctggcaccgt taatcagcgt cctgccggtt tcaggcggtt
     4381 tgctgggcct ttccggtatt ctggcaagcc aggcagagag cgtttgtgaa gcttatgccg
     4441 atagcttcgc actggacccg gtcgtggaaa aagaagagtg gtgccgtatt accggtcgta
     4501 agaattaacc ttcgcatcgc cgtagggtga cgcggggcga agtgcgagca agctcacaaa
     4561 aggcacgtaa atttgccggt tatttacgca aatttgcgtg ccaaaatttt cattcataaa
     4621 gaaaaattga gaacttactc aaatttcttt gagtgtaaat tttagtcact attttctaat
     4681 atgatgattt ttatgagtaa ttatcgcacc acgctcattt taaatgcaat tctttgatcc
     4741 atctcagagg attggtcaaa gtttggcctt tcatctcgtg caaaaaatgc gtaatatacg
     4801 ccgccttgca gtcacagtat ggtcatttct taactcatgc gcatcggaca atatcagctc
     4861 agaaatcgcc tgatcgcagc gcccatggct ggcattacag acagaccttt tcggacgttg
     4921 tgctacgaga tgggagccgg attgacagta tccgagatga tgtcttctaa cccacaggtt
     4981 tgggaaagcg acaaatctcg tttacggatg gtgcacattg atgaacccgg tattcgcacc
     5041 gtgcaaattg ctggtagcga tccgaaagaa atggcagatg cagcacgtat taacgtggaa
     5101 agcggtgccc agattattga tatcaatatg ggttgcccgg ctaaaaaagt gaatcgcaag
     5161 ctcgcaggtt cagccctctt gcagtacccg gatgtcgtta aatcgatcct taccgaggtc
     5221 gtcaatgcag tggacgttcc tgttaccctg aagattcgca ccggctgggc accggaacac
     5281 cgtaactgcg aagagattgc ccaactggct gaagactgtg gcattcaggc tctgaccatt
     5341 catggccgta cacgcgcctg tttgttcaat ggagaagctg agtacgacag tattcgggca
     5401 gttaagcaga aagtttccat tccggttatc gcgaatggcg acattactga cccgcttaaa
     5461 gccagagctg tgctcgacta tacaggggcg gatgccctga tgataggccg cgcagctcag
     5521 ggaagaccct ggatctttcg ggaaatccag cattatctgg acactgggga gttgctgccc
     5581 ccgctgcctt tggcagaggt taagcgcttg ctttgcgcgc acgttcggga actgcatgac
     5641 ttttatggtc cggcaaaagg gtaccgaatt gcacgtaaac acgtttcctg gtatctccag
     5701 gaacacgctc caaatgacca gtttcggcgc acattcaacg ccattgagga tgccagcgaa
     5761 cagctggagg cgttggaggc atacttcgaa aattttgcgt aaacagaaat aaagagctga
     5821 cagaactatg ttcgaacaac gcgtaaattc tgacgtactg accgtttcta ccgttaactc
     5881 tcaggatcag gtaacccaaa aacccctgcg tgactcggtt aaacaggcac tgaagaacta
     5941 ttttgctcaa ctgaatggtc aggatgtgaa tgacctctat gagctggtac tggctgaagt
     6001 agaacagccc ctgttggaca tggtgatgca atacacccgt ggtaaccaga cccgtgctgc
     6061 gctgatgatg ggcatcaacc gtggtacgct gcgtaaaaaa ttgaaaaaat acggcatgaa
     6121 ctaattcagg ttagctaaat gcttgattaa aaaggcgcta ctcggcatgg ggaagcgcct
     6181 tttttatagg tgtcacaaag ggagtgacca tgagaacagg atgtgaaccg acccggtttg
     6241 gtaatgaagc taagaccatt attcacggtg atgcgcttgc cgaacttaaa aagatccccg
     6301 ccgaaagtgt cgatctgatc tttgccgacc caccatataa catcggtaaa aattttgatg
     6361 gtctgatcga agcctggaaa gaagatctgt ttatcgactg gctgtttgaa gtgattgcag
     6421 agtgccaccg cgttctgaaa aagcagggca gcatgtacat catgaacagc acggaaaaca
     6481 tgccctttat cgatctccag tgccgcaagc tttttaccat caaaagtcgc atcgtctggt
     6541 catatgacag ttctggagta caggcgaaaa aacactacgg ctccatgtac gaacccatcc
     6601 tgatgatggt gaaagacgca aagaactaca cattcaacgg tgatgctatt ctggtagaag
     6661 ccaaaaccgg atcgcagcgc gcgttgatcg attatcgcaa aaatcctcca cagccataca
     6721 atcatcaaaa agtaccgggt aacgtctggg attttccgcg cgtgcgttat ttgatggatg
     6781 aatatgagaa ccacccgacg caaaaaccgg aagccttact gaaacgcatt attctcgcct
     6841 cttccaaccc aggcgatatc gttctcgacc cgtttgccgg tagctttact accggtgccg
     6901 tagccgtctc cagcgggcga aaattcattg gtattgagat caacagcgag tacatcaaaa
     6961 tggggcttcg acggctggat gtcgcgtcgc attactctgc ggaagaactg gcgaaagtga
     7021 aaaaaagaaa gaccggcaac ctgtcaaaac gaagccggtt aagcgaagtt gaccccgatc
     7081 tcattgcaaa gtaaaggaag tgtaagcctg gttttcagat tattcatttc gtgtatattt
     7141 cctgccagac ttggttaaac atacacaggc tcaggtaatg attcgcaagt attggtggct
     7201 cgtcgttttc gctgtcttcg tttttctgtt tgatacttta ctgatgcagt ggattgaact
     7261 gctggcaaca gaaacagaca aatgccgcaa tatgaactca gttaatccac taaaactggt
     7321 taactgtgac gaactgaatt ttcaggacag aatgtgaatt tacatgacac ttaattcatt
     7381 cgtttgatga attaatttcg ttatgttttc atctggcatg aacattctta atacgttatc
     7441 gaccagagcg ggggcttgtt tataaagatc ataacccgcc atattcatta accagttttg
     7501 aacaattccg ctgaaggcac cattaataat aatcatcaca acatctaaat cgaggttatt
     7561 tgctacacaa ccttgttgct gacacgcctg caatacttcg cggagagtct gcggattgaa
     7621 gcccatcttt tcgcgtatca ctccctcggc cagcatctca tcattaaatt cacatttgtg
     7681 atataagatt ttcagcaacg cctgctggcg gggaattttg gcaatatatt gcaagccgac
     7741 aatcaatttt tcacgcaatt gttgaaacgg gtcatgctct aatccagccg tcaagtgttc
     7801 ctggattaac tcccgcaatg aaggctgttg caaccacatc tcattaaaca gttgagtctt
     7861 gttttcgaag tgccagtaga tagcgccacg cgtaacgtta gcagcgtcgg caatgtcgtt
     7921 gagtgtcgtc ttgcttacgc catgctgcgc aaactgggcg atggcagttt caatcagttc
     7981 ttgccgggtc ttcagagctt cggctttggt tctttttgcc atgattaatt attcaggaaa
     8041 taaatatatt cgacacagag tgagaaaata gcgaaggtta atctatcacc taatgtgt
//
LOCUS       DAZGLJ010000195         8077 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000195 DAZGLJ010000000
VERSION     DAZGLJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.195"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            546..1829
                     /gene="ydfJ"
                     /locus_tag="ACHK49_003889"
                     /pseudo
     CDS             546..1829
                     /gene="ydfJ"
                     /locus_tag="ACHK49_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            1918..3378
                     /gene="ydfI"
                     /locus_tag="ACHK49_003890"
     CDS             1918..3378
                     /gene="ydfI"
                     /locus_tag="ACHK49_003890"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO9264445.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPAIGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNLIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(3414..3617)
                     /gene="ydfZ"
                     /locus_tag="ACHK49_003891"
     CDS             complement(3414..3617)
                     /gene="ydfZ"
                     /locus_tag="ACHK49_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO9264446.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(3795..4481)
                     /gene="rspR"
                     /locus_tag="ACHK49_003892"
                     /gene_synonym="ydfH"
     CDS             complement(3795..4481)
                     /gene="rspR"
                     /locus_tag="ACHK49_003892"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO9264447.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(4570..5316)
                     /gene="ydfG"
                     /locus_tag="ACHK49_003893"
     CDS             complement(4570..5316)
                     /gene="ydfG"
                     /locus_tag="ACHK49_003893"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO9264448.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5453..7498
                     /gene="dcp"
                     /locus_tag="ACHK49_003894"
     CDS             5453..7498
                     /gene="dcp"
                     /locus_tag="ACHK49_003894"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO9264449.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHSVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(7543..8061)
                     /gene="ydeJ"
                     /locus_tag="ACHK49_003895"
     CDS             complement(7543..8061)
                     /gene="ydeJ"
                     /locus_tag="ACHK49_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO9264450.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         2038 a         1847 c         2059 g         2133 t
ORIGIN      
        1 tgttcttaaa gttcgtagat aaacattgtc gtttcaggta tacaggaatg ctaacaggtg
       61 gcagcaaaaa tcaggcggtt tatggcgcaa gctgaagcgg caactgcaaa ctatcttatg
      121 cagagactct acacggattg ggtttaaaag tatacataga taacagtttt tatctgaaaa
      181 agaaaaatat caaggtgata tagcctatat gcctttgatg tggaggaatg aatgtgatgg
      241 gagtgatgta tctgaatagt tgaaaaaccg cagacacacc ttatgcaaga acgtgctgcg
      301 gttggctggt aaatttttcg atagtgcgag tattgaatga tttccagccg ttattgattt
      361 tacgcgtaaa tccatgaaaa aactacttat ctgttgggga gttttttggg gcatatatgg
      421 gacagaaata ggccccagat agacactgag atcgaactta ggatgctttt aaaaaaatgc
      481 aactatctga aaaaacctag aaaacgccaa ggaaaccaca ggatgggaaa aaacacctgt
      541 gaattatgga tttccagtta tattcgctcg gcgcagcgtt agtgtttcat gaaatatttt
      601 ttcctgaatc atcaacggca atggcgttaa ttttggcaat gggaacctac ggtgcaggtt
      661 atgtggcgcg tattgtcgga gcatttattt tcggcaaaat gggcgacaga atagggcgta
      721 aaaaagtgct ctttattacc atcaccatga tggggatctg taccacctta attggtgtgt
      781 taccgaccta tgcacagatt ggtgtttttg cccccatctt gctggtgacg ctgcgtatta
      841 ttcaggggtt gggtgcaggt gcggaaattt ccggtgccgg tacgatgctg gcggaatatg
      901 cgccaaaagg taagcgcgga attatctcct catttgtagc tatgggaact aactgcggaa
      961 ccttgagcgc aacggcaatc tgggccttta tgttcttcat tctcagtaaa gaggaactgc
     1021 ttgcgtgggg atggcgtata ccgttcctgg cgagcgttgt cgtgatggtc tttgctatct
     1081 ggttgcgtat gaatctgaaa gaaagcccgg tctttgagaa ggttaacgac agcaaccaac
     1141 ccacagcaaa acctgcacct gctggtagca tgttccagag caaatccttc tggctggcaa
     1201 cagggctgcg ttttggtcag gcgggtaact ccgggttaat tcagactttc cttgcaggct
     1261 atttagtgca gacgttattg tttaacaaag caattccaac agatgcattg atgattagtt
     1321 cgattctcgg ctttatgacc attccgttcc ttggttggtt atccgataaa attggtcgcc
     1381 ggatcccgta tattattatg aatacctccg cgattgtgct ggcatggcca atgctttcta
     1441 tcattgtaga taaaagctat gccccgagca ccattatggt tgcactgatt gtgattcata
     1501 actgtgcggt gctgggatta tttgctctgg aaaacattac catggcagaa atgttcggct
     1561 gtaaaaaccg ctttacccgg atggctattt ctaaagaaat tggtggtctt atcgcttccg
     1621 gttttggtcc tatcctggcg ggtattttct gcaccatgac ggaatcctgg tatccgatcg
     1681 ccattatgat catggcatat tcagtaattg gtttaatctc tgcgctgaaa atgccagagg
     1741 tgaaagaccg tgatttaagt gcgctggaag acgctgcgga agatcaaccg cgtgttgtaa
     1801 gagctgcgca accttccaga agtctgtaaa tcctcaatcc cttctcttac cggagagggg
     1861 atttttattc atataacaaa acatatagct tgccatattt atatttaagg aattgttatg
     1921 ggaaataatt tgttatcagc aaaagcgaca ctccctgttt atgatcgtaa taacctggcc
     1981 ccaagaatta ttcatttagg ctttggtgca tttcaccgtg cgcatcaggg cgtgtatgcc
     2041 gatattcttg ctacagaaca tttcagtgac tggggatatt atgaggtcaa cttaatcggc
     2101 ggcgaacagc aaattgccga tttacaacag caagataatc tttataccgt tgcggaaatg
     2161 tcggccgatg cgtggacggc tcgcgtcgtt ggcgtcgtta aaaaagcctt gcacgtacag
     2221 atggatggct tagaaaccgt gttggctgcg atgtgtgaac cgcaaatcgc gattgtctct
     2281 ctgacaatca ccgaaaaagg gtatttccac tctccggcga tcggacagtt aatgctcgat
     2341 cacccgatgg tcgctgccga cgtgcaaaat ccccaccagc cgaaaacagc aacaggagtg
     2401 attgttgagg cgctggcccg ccgtaaagcg gcaggacttc ccgcatttac cgtcatgtca
     2461 tgtgacaaca tgccagaaaa cggtcatgtt atgcgtgacg ttgtcacttc atacgcgaaa
     2521 gctgttgatg taaaactggc acaatggatc gaagataacg tgactttccc atcaacaatg
     2581 gtggaccgta ttgtgcccgc agtgacagag gatacgctgg cgaaaatcga acaacttacc
     2641 ggtgtgggcg atcctgcggg cgttgcctgt gaacctttcc gccagtgggt aatagaagat
     2701 aactttgttg ccggacgtcc agaatgggaa aaagcgggag ccgaactggt tagtgatgtg
     2761 ctgccttatg aagagatgaa gttgcgcatg ctcaacggca gtcattcatt cctggcgtat
     2821 ttgggttatc ttgctggata tcagcacatt aatgactgca tggaagatga acattatcgt
     2881 tatgcggcgt atggcttgat gttgcaggaa caagcgccga cgctgaaagt gcagggcgtt
     2941 gatttgcaag attacgctaa ccgattaatt gcacgctata gcaacccggc gttacgtcat
     3001 cgaacctggc agattgcgat ggatggtagc cagaaattgc cacagcggat gttggattct
     3061 gttcgctggc atctggcgca tgacagcaag ttcgatctgc tggcgctggg cgtcgcgggt
     3121 tggatgcgtt atgtcggtgg tgttgatgaa cagggaaatc tgatagaaat cagtgaccca
     3181 ctgttacctg ttattcagaa ggctgtacaa agtagtgccg aagggaaagc gcgcgtccag
     3241 tcattgctgg cgattaaggc aatctttggt gatgatttgc cagacaatag cttgtttact
     3301 gcaaaagtga cggaagcgta cttgtcttta ttagcgcatg gcgcgaaagc gaccgtggcg
     3361 aaatattccg tgaagtaaaa aaagcaatgc cgccatcggc ggcattcaca cacttagttc
     3421 atgccgagac gaatcaggtc cagaggtgcc agtttctctt cacaaccttc aacaactacg
     3481 gtttttccgc ggcggatttg ctcagcggtc agaccttcag tatcaatcga caggatcttg
     3541 ccagtgtgac cggtgccgct aaccataaca cggctgccag tggtgattgc gttacggtta
     3601 cgatcgtagg tcgtcatagg tgttttctcc tttctgattt acagtcgaag cccacgtaac
     3661 gggcgcaata taaatactcc tgtcgagtga aatatttttt gtttttgatc aagctcacat
     3721 cttttttgat gcatgcatga gccgtaaggc ggggggaaaa gcctctcatc ccggatggga
     3781 tgagagtaaa tgaattactc ttcgctaaac cagtcgctgt tttcctggcg gatctggcgc
     3841 acggattcgc tgatttcctg caaatgttgc gtcattgcac gttctaccgc atcgccatca
     3901 cgtttttgca gggcagagaa aatatcaaga tgctggcgta acagcatttc tggtggagaa
     3961 acatggtcga aactcatata gcgcacgcga tcaacggtcg ctttcaggtt ctcaatggta
     4021 tcccacgcca gttgacagtc ggcaatctgc gtcaggagtt gatggaagtt gtcatcaagt
     4081 tcaaaaaaat catccagttg cttgcgctca atggcaatgc gttgctggtg aagattttgt
     4141 tccagttggt agcactggct ttcggtaatc atgctcgccg cccgacgcgc caccgcgcac
     4201 tcaatggcct gacggataaa actgccgttt cgcacctggg ccatggaaat tttgttgacg
     4261 tagctgccac gctgcggacg aatttgaatc aggccgtttt ccgccagttt aataaaggct
     4321 tcacgaaccg gctggcgtga cacattgaaa cgaacagaaa cttctttttc cgacaacggt
     4381 gtgcctggtg caatcaggca atggacaatg tcgcgacgaa gaatacgata aatctgctga
     4441 ttgacaggct gtgtgggatt aagttgcgtt tcgacggtca ttcgttcttg cttgtgagtg
     4501 agttaactgc atgagtctac cacttttttg tgacaagcaa taacccggca gttacgccgg
     4561 gtataaaaat tactgacggt ggacattcag tccggcatag ctttgggtaa ccggcatcat
     4621 ttccagggta ttgatattga cgtgagcagg cagcgttgac acccaccaga cggcttcgct
     4681 gacatcttct ggcgtcaatg caacggtatt ttgataggtt ttttccgctt taccgtcatc
     4741 gcctttaaag cggacattgg aaaactcggt gccacccacc agacccggtt cgatgtcggt
     4801 gacgcgcacc gccgtaccat gcaggtccgt acgcagattc aggctaaact gacgaacaaa
     4861 cgctttcgtc gcaccgtaaa cgttaccacc ggcataaggc cagctacctg ccgttgagcc
     4921 aatgttaata atatgaccat gattacgttc aaccataccc ggtaagacgg cgcgcgtcat
     4981 atataccagg cctttgttgt tggtatcaat catcgtttcc cagtcttcaa cgctggcttt
     5041 atgcgcaggc tccatgccca acgccaggcc ggcattattt accaggatat caatattgca
     5101 ccactcggca gggagcgatg ccagcatctc ttcaatagcg gcgcggttgc gaacgtccag
     5161 ttgggcgata tacagattat ctcccagttc gtcttttaac tcctgcaacc gctcctggcg
     5221 acggccagtg gcgataactt tatgcccttg ttgaataaaa cgacgagtaa tgcattcacc
     5281 aaaacctgcc gttgctccag ttactaaaac gatcatctca ctgttcctca acgcttttgt
     5341 gtgtcaataa catagcacgt gatgcttaag agcgggtaat actggtttgc aagattgcac
     5401 tccgttatca gtaagcctac gctatggaac acctcgcgtt caggagaaga aaatgacaac
     5461 aatgaatcct ttccttgtgc aaagcacact gccgtatctg gctcctcatt ttgatcaaat
     5521 tgccaatcat cactatcgcc cggcattcga tgagggaatg cagcaaaagc gggcagaaat
     5581 tgctgccatc gcgcttaacc cgcaaacgcc tgatttcaac aatactattc tggcactgga
     5641 acaaagcgga gaattactta cccgcgttac cagcgtcttt tttgcgatga ctgcggcgca
     5701 taccaatgat gaattacagc gtcttgatga acagttttcc gctgaactgg cggaactggc
     5761 taatgatatc tatctgaacg gtgaattatt cgcgcgggta gatgctgtct ggcagcgccg
     5821 tgaatccctg gggcttgata gtgaatccat ccgcctggtg gaggtgattc atcaacgttt
     5881 tgtccttgcc ggagccaaac ttgcgcaagc tgataaagca aaattaaaag tactgaatac
     5941 agaagctgcg acactgacca gtcagtttaa ccagcggtta ctggcagcaa ataaatccgg
     6001 cggtctggtt gtgaacgata tcgcgcagct ggcaggaatg agtgagcaag agattgcgct
     6061 ggcggcagag gcggctcgcg agaaaggtct ggataacaaa tggctgattc cgctgctgaa
     6121 taccacccaa caaccggcgc ttgccgaaat gcgcgatcgt gcgacgcgtg aaaaactgtt
     6181 tattgcgggc tggacgcgag cggaaaaaaa tgatggcaat gatacccgcg ctatcattca
     6241 acgtctggtg gagatccgtg cacaacaggc gacattactt ggttttcctc attatgccgc
     6301 atggaaaatc gccgatcaga tggcaaaaac acctgaagca gcgctcaact ttatgcggga
     6361 aattgttcca gcggcgcgtc aacgtgcgag cgatgaatta gcctccatac aggcggttat
     6421 tgataagcag cagggcgggt ttagcgcgca gccgtgggac tgggcatttt atgccgaaca
     6481 ggtacggcgg gagaaatttg atcttgatga ggcgcagctc aagccatatt ttgaattaaa
     6541 cacggtgttg aatgaaggtg tattctggac cgcgaatcag ctcttcggta ttaagtttgt
     6601 cgaacgtttt gatattcctg tctaccatcc tgacgttcgt gtgtgggaaa tttttgatca
     6661 taatggcgtg ggactggcgt tattttacgg tgatttcttc gcccgtgatt caaaaagcgg
     6721 cggtgcatgg atgggcaatt ttgttgagca atcaacgctt aatgaaacgc attcggtaat
     6781 ttataacgtt tgcaattatc agaaacccgc tgccggtgag cctgcgttgt tactctggga
     6841 tgatgtcata accttattcc atgaatttgg tcatacgctg cacggccttt ttgcccgcca
     6901 gcgttatgcc acgctttccg gcaccaacac gccgcgtgat tttgtcgaat ttccgtcgca
     6961 aatcaacgaa cactgggcaa cgcatccgca ggtattcgct cgctacgccc ggcattatca
     7021 gagcggggca gcaatgcctg acgaactgca acagaaaatg cgtaatgcca gcctgttcaa
     7081 caaagggtat gagatgagcg aactgcttag cgccgcactt ctcgatatgc gctggcattg
     7141 cctggaagaa aacgaagcaa tgcaggatgt cgatgatttt gaattgcggg cgctggtggc
     7201 ggaaaatatg gatcttcctg ctataccgcc acgctatcgc agcagttatt tcgcccatat
     7261 ttttggtggc ggatatgccg caggttatta cgcttatctg tggacacaaa tgttggccga
     7321 tgatggttat cagtggtttg ttgagcaggg cggattaacg cgtgaaaatg ggctgcgttt
     7381 tcgcgaggcg atcctttcca gaggtaacag cgaagatctg gaacgcctgt atcgacaatg
     7441 gcgcggtaag gctcctcaga ttatgccgat gctgcaacat cgtggcttga acatataaac
     7501 accaccaatg gctgtcctgg agatatcgcc aggccagcct ggttatagca gtaattgcag
     7561 cagctgggcg agggcaaacc tcaccgctaa agctaatacc gtttcgcagt cgccagcaaa
     7621 atgcataacc gcagtatagg tctggccttt aatatgccac gcgaaccaga cggtacccgc
     7681 tggcgtacca tcttcaccgc cctccggtcc gccgtagccg gtaatggcaa tacttacatc
     7741 agcatccgca cgctctatgg ctccggttgc catttctgct gccactttct cactcaccgc
     7801 agaatatcgt tcaagagatt gctggcttac gctgaggatt ttcatctttg cctgatcggt
     7861 gaaagtaaca aagcctgcac cgtaaaattt gggtgtatct tcagctgcac acagggcgct
     7921 agccaactta ccgccggtgc atgattcggc ggtagttaag cgtaattgat ccgcgataag
     7981 tctttgactc aacgcggatg tcaggttttc aatagtgtcg gtatctgcga gttgtacaat
     8041 tttatcttta ttaatgttca ctgttttttg tccacgc
//
LOCUS       DAZGLJ010000196         8076 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000196 DAZGLJ010000000
VERSION     DAZGLJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.196"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..329
                     /locus_tag="ACHK49_003896"
     CDS             <1..329
                     /locus_tag="ACHK49_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO9264451.1"
                     /translation="WEHYKAGADEEALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNI
                     NMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPA
                     LTQEVA"
     gene            329..1021
                     /gene="rutB"
                     /locus_tag="ACHK49_003897"
     CDS             329..1021
                     /gene="rutB"
                     /locus_tag="ACHK49_003897"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO9264452.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            1033..1419
                     /gene="rutC"
                     /locus_tag="ACHK49_003898"
     CDS             1033..1419
                     /gene="rutC"
                     /locus_tag="ACHK49_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO9264453.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLEIIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            1427..2227
                     /gene="rutD"
                     /locus_tag="ACHK49_003899"
     CDS             1427..2227
                     /gene="rutD"
                     /locus_tag="ACHK49_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO9264454.1"
                     /translation="MKLSLSPPPYADTPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            2237..2827
                     /gene="rutE"
                     /locus_tag="ACHK49_003900"
     CDS             2237..2827
                     /gene="rutE"
                     /locus_tag="ACHK49_003900"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO9264455.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            2838..3332
                     /gene="rutF"
                     /locus_tag="ACHK49_003901"
     CDS             2838..3332
                     /gene="rutF"
                     /locus_tag="ACHK49_003901"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO9264456.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            3353..4681
                     /gene="rutG"
                     /locus_tag="ACHK49_003902"
     CDS             3353..4681
                     /gene="rutG"
                     /locus_tag="ACHK49_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO9264457.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(4764..4937)
                     /gene="ymdF"
                     /locus_tag="ACHK49_003903"
     CDS             complement(4764..4937)
                     /gene="ymdF"
                     /locus_tag="ACHK49_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO9264458.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            5310..5906
                     /gene="wrbA"
                     /locus_tag="ACHK49_003904"
     CDS             5310..5906
                     /gene="wrbA"
                     /locus_tag="ACHK49_003904"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO9264459.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            5927..6154
                     /gene="yccJ"
                     /locus_tag="ACHK49_003905"
     CDS             5927..6154
                     /gene="yccJ"
                     /locus_tag="ACHK49_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO9264460.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(6192..7433)
                     /gene="agp"
                     /locus_tag="ACHK49_003906"
     CDS             complement(6192..7433)
                     /gene="agp"
                     /locus_tag="ACHK49_003906"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO9264461.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(7725..>8076)
                     /locus_tag="ACHK49_003907"
     CDS             complement(7725..>8076)
                     /locus_tag="ACHK49_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264462.1"
                     /translation="NDKKCILHLPEFIFNDNKARLVLDTDPEQKVNEVKNFLTALSDS
                     ICSLFIVNGNVASISLGNESFSTDDDLEYGYLINTGNHYDVYLPPELFAQAYELNNKE
                     RNAQIDFLTRYAIY"
BASE COUNT         1825 a         2063 c         2171 g         2017 t
ORIGIN      
        1 aatgggaaca ctacaaagcg ggcgcggatg aagaggcgtt aagctggcta accgaacaaa
       61 gtcagaaaga tacccgctcc ggtactgata ccaacgtccg tcagatggcc gatcccactt
      121 cggcggtaaa catcaatatg gggacgttag tcggttctta cgccagtgtc gcgcgcatgt
      181 tagatgaagt cgcaagcgtg cctggtgccg aaggcgtgct gttgaccttc gatgattttc
      241 tgtcgggaat cgaaaccttc ggcgagcgca ttcaaccact gatgcagtgc cgcgcccatc
      301 tacctgcgct gactcaggag gtggcatgat gacaacctta accgctcgac cggaagccat
      361 taccttcgat ccgcagcaaa gtgcgctgat cgtggtggat atgcaaaacg cttatgccac
      421 gccaggcggc tacttagatc tcgccgggtt tgatgtctca accactcgcc cggtcattgc
      481 caacattcaa accgccgtga ccgcagcgcg agcggcaggg atgctgatca tctggtttca
      541 aaatggctgg gatgaacagt atgtcgaagc tggcggcccc ggctcaccga attttcataa
      601 atcgaacgcc ctgaaaacca tgcgtaagca gccgcagctg caggggaaat tgctggcgaa
      661 aggctcctgg gattatcaac tggtggatga actggtgccg cagcctggcg atattgtgct
      721 gccgaagccg cgctacagcg gtttcttcaa tacaccgctg gacagcattt tgcgcagccg
      781 tggaatacgc catctggttt tcaccggtat cgctaccaac gtctgcgtcg aatcgacgct
      841 acgcgatggc ttttttctgg agtatttcgg cgtggtgctg gaagacgcaa cacaccaggc
      901 ggggccggaa tttgcacaga aagccgcgtt gttcaatatc gaaacctttt ttggctgggt
      961 cagcgacgtc gaaacgttct gcgacgcgct ttctcccaca tcctttgctc gtatcgctta
     1021 aggagtttaa cgatgccaaa atccgtaatt attcccgctg gcagcagcgc accgctggcc
     1081 cccttcgttc ccggcacgct ggctgatggc gtggtgtatg tctccggtac gctggctttt
     1141 gatcaacata ataacgtgct gtttgccgat gacccaaagg cgcaaacccg ccacgttctg
     1201 gaaattatcc gcaaggtgat cgagacggcg ggtggcacga tggcggatgt gaccttcaac
     1261 agcatcttta ttaccgactg gaaaaattac gccgcgatta acgaaatcta cgccgagttt
     1321 tttccgggtg ataaaccggc gcgattctgc attcagtgcg gactggtaaa acctgacgcg
     1381 ctggtggaaa tcgccacaat tgcgcatatc gccaagtgag gccgcgatga aactttcact
     1441 ctcacctccc ccttatgctg atacgcccgt agtggtgttg atttcgggtc ttggcggtag
     1501 cggcagttac tggttaccgc aactggcggt gctggagcag gagtatcagg tagtctgtta
     1561 cgaccagcgc ggcaccggca ataatcccga cacgctggca gaagattaca gtatcgccca
     1621 gatggcagcg gaactgcatc aggcgctggt agccgcaggg attgagcatt acgcggtggt
     1681 cggccatgcg ctcggtgcgc tggtgggaat gcagctggcg ctggattatc ccgcgtcggt
     1741 aactgtgctg atcagcgtta acggctggct acgaataaac gcccatacgc gccgctgttt
     1801 tcaggttcgc gaacgattac tgtatagcgg cggcgcgcag gcatgggtgg aagcgcagcc
     1861 gttgttcctc tatcccgccg actggatggc ggcccgcgca cctcgcctgg aggcagaaga
     1921 cgcgctggca ctggcgcatt ttcagggcaa aaataattta ctgcgtcgac ttaacgccct
     1981 caaacgcgct gactttagtc accatgcgga tcgcatccgc tgcccggtgc aaatcatctg
     2041 cgccagtgat gatctgctgg tgccatcagc atgttccagt gagcttcatg ccgccctgcc
     2101 cgatagccag aaaatggtga tgccctatgg cggacacgcc tgcaacgtga ccgatcccga
     2161 aacgtttaat gctctgttac tcaacgggct tgccagcctg ttacatcacc gtgaagccgc
     2221 cctgtaagga attgctatga acgaagccgt tagcccaggt gcgcttagca ccttgttcac
     2281 cgatgcccgc actcacaacg gctggcggga gacacccgtc agcgatgaga cgttacggga
     2341 gatttatgcc ctgatgaaat gggggccgac atcagctaac tgttctccgg cacggatcgt
     2401 gtttatccgc acggcagaag gaaaagaacg tctgcgcccg gcactttcca gcggcaatct
     2461 gcaaaaaacc ctgaccgcgc ccgtcaccgc tatcgtcgcc tgggacagtg aattttatga
     2521 acggttaccg caactgtttc ctcacggcga tgcccgcagt tggtttacct ccagcccaca
     2581 acttgccgaa gaaacagcgt ttcgcaacag ttccatgcag gcggcctatc tgatcgtcgc
     2641 ctgccgtgcg ctgggactgg ataccggccc gatgtcgggt tttgaccgtc aacacgttga
     2701 cgacgccttt tttgcgggca gcacgctgaa gagcaatctg ctgattaata tcggctatgg
     2761 cgatagcagc aaactttttg cgcgcctgcc acgtctgtcc tttgaagaag cctgcgggct
     2821 gttgtaagga gcaaaccatg aacattgtcg atcaacaaac ctttcgcgat gcgatgtcct
     2881 gtatgggcgc ggcagtcaat atcatcacca cggatggtcc agccgggcgc gccgggttca
     2941 ccgccagcgc ggtctgcagc gtgaccgaca cgccgcctac attactggtg tgcctgaatc
     3001 gtggggcgtc cgtctggccg gtattcaatg aaaatcgaac gctgtgtgta aatacactga
     3061 gcgccgggca ggagccgctt tcaaatcttt ttggcggcaa aacgcccatg gaacaccgct
     3121 ttgccgccgc ccgctggcag acgggtgtga ccggatgccc gcaactggaa gaggctctgg
     3181 tttcgtttga ctgccgtatc agccaggtgg tgagcgtcgg cacccacgac attctgtttt
     3241 gcgccatcga agcgattcat cgtcacgcca caccctacgg gctggtgtgg ttcgatcgca
     3301 gttatcacgc gctgatgcgc cctgcttgtt aacctcctaa aggagacagc tgatggcaat
     3361 gttcggtttt cctcactggc agttaaaatc gacctctaca gagagcggcg tggtcgcgcc
     3421 gtatgaacga ttgccgtttg ctcagacggc agtaatgggc gttcaacacg cggtggcgat
     3481 gtttggcgcg acggtgttaa tgccgattct gatggggctg gatcccaatc tttccatttt
     3541 aatgtcgggg atcggcacgt tactgttttt ctttattacc ggcgggcgcg tccccagtta
     3601 tctcggttcc agcgcggctt ttgtcggcgt ggtgattgcc gcgacgggct ttaacggtca
     3661 gggcattaac ccgaatatca gcattgcgct gggcgggatt atcgcctgtg ggctggttta
     3721 taccgtcatt ggcctggtgg tgatgaaaat tggtacacgc tggattgaac gactaatgcc
     3781 gccggtggtg acgggtgcgg tggtgatggc gattggcctg aacttagcgc ctatcgcagt
     3841 gaaaagtgta tcggcttcgg cctttgatag ctggatggcg gtaatgacgg tgctgtgtat
     3901 tgggctggtg gcggtgttta cccgtggcat gatccagcgg ttattgattc tcgtaggttt
     3961 aattgtcgcc tgtctgctgt atggcgtgat gaccaacctt ttagggctgg gaaaagccgt
     4021 cgattttact ctcgtcagcc atgctgcctg gttcggtttg ccacattttt ccactcctgc
     4081 ttttaatagt caggcaatga tgctgattgc gcccgtggcg gtgattctgg tggcggaaaa
     4141 cttaggtcat ctcaaggcgg tcgccgggat gaccggacgc aatatggacc cgtatatggg
     4201 gcgggcattc gtcggtgatg ggctggcgac gatgctttct ggttctgtcg gtggcagcgg
     4261 cgtaaccacc tacgccgaga atatcggtgt aatggccgtg acaaaagtct actcgacgct
     4321 ggtgtttgtc gctgctgccg tcatcgccat gctgttgggc ttttcaccga aatttggcgc
     4381 gctgattcat accattccgg ccgcggtgat tggtggtgca tcaattgttg tgttcgggct
     4441 gattgccgtc gcaggcgcaa ggatatgggt acaaaaccgt gtcgatttaa gccagaacgg
     4501 taatttgatt atggtcgcag taaccttagt gctgggcgcg ggtgattttg ctctcacgct
     4561 gggcggtttt acgttgggag ggattggtac agcaaccttt ggcgcgattt tactcaatgc
     4621 gttgttaagc agaaagttgg ttgacgttcc accgccagaa gttgttcatc aggagccata
     4681 aacagaaaac cgccgggatt accggcggtg atgacttttt gacgagccta agaaaaaaat
     4741 gcattcgtca ttgattagcc cggctagttg tcgcttttgc cgtgactgct cttgccacct
     4801 tttttacctg cttcagatgc gcgctgcggg tcattcttga aattaccccc gctgtgctgt
     4861 ccaccttttt tacctgcttc tgatgctctt tcgcggtctt ctgcaaaatt gccggaaccg
     4921 cctcgatggt ttgccattac tgacctccgt ctgggtatag acatcatatt gcatagaatt
     4981 aacgcggcat atggcggctg ccatattttc gctacgtggg actaagctta gtgaactgac
     5041 gcgggcagac ccgcgtttgg taatatcctg caacagcttc ttagcgagaa tagatatcat
     5101 ctcaaataat tatccataag ccgctcttat gattaaaaaa acgcgatgag ataaaaatgt
     5161 gtcattttgc gacaaaatta cgtgcttgtg aaagttgtta taaatcaaat aagtggttgt
     5221 gaaatttgca ctctgaaaag gacgtcttat ctttaaataa gtggtagcga atcgctacgg
     5281 aatagagata acacgaggag tggttagaaa tggctaaagt tctggtgctt tattattcca
     5341 tgtacggaca tattgaaacg atggcacgcg cagtcgctga gggtgcaagc aaagtcgatg
     5401 gcgcagaagt tgtcgttaag cgtgtaccgg aaaccatgcc gccgcaatta tttgaaaaag
     5461 caggcggtaa aacgcaaact gcaccggttg caaccccgca agaactggcc gattacgacg
     5521 ccattatttt tggtacacct acccgctttg gcaacatgtc cggtcaaatg cgtaccttcc
     5581 tcgaccagac gggcggcctg tgggcttccg gcgcactata cggaaaactg gcgagcgtct
     5641 ttagttccac cggtactggc ggcggtcagg aacaaactat tacttcaacc tggacgaccc
     5701 ttgcgcatca cggcatggta attgtcccca ttggctacgc agcgcaggaa ttatttgacg
     5761 tttcacaggt tcgcggcggt acgccgtacg gcgcaaccac catcgcaggc ggtgacggct
     5821 cacgccagcc aagccaggaa gaactgtcta ttgctcgtta tcaaggggaa tatgtcgcag
     5881 gtctggcagt taaacttaac ggctaatctt caacaggagg atacgcatgc caactcaaga
     5941 agcgaaagct catcacgtcg gtgaatgggc atctctgcgc aatacgtcgc cggaaatagc
     6001 cgaggccatt ttcgaagtcg ccgggtacga cgaaaaaatg gcggaaaaga tttgggaaga
     6061 aggtagcgat gaagtgttag tcaaagcctt tgctaagacc gataaagatt cgcttttttg
     6121 gggcgaacag accatcgaac gtaaaaacgt ttaaccagcg actcccccac ttctcgcggg
     6181 ggagttttct gttatttcac cgcctcattc aacacgctat caaacttatc cattgggcag
     6241 aaaccgttgg catctatcgg gcaaccgctt aattccagcg tcacacgctg agcaggtgcc
     6301 tgcagggtta acgcatcggc attacgtaac tgttccgcac tctgatacac atattcaatt
     6361 ttcatcaaat cgcgattggc tttgctgtca tgccaacgct ggaaaacgat tttgccgcca
     6421 atcggcgtgc gttcgttctg gtcatgcaac tgatacggtt tgaaatccag cgccgttaac
     6481 agagaggcaa tgttggagtc gtgcccaacc aacactgtaa ttttcggtgc gctggtgcga
     6541 tcggtgacca gagctttgtc gatataactg accagcggtt tcgcaacatt gcgcgccact
     6601 tccggtgagg taaacaggct gtcctggtag ccgtttttaa gcttcgacaa caccttccac
     6661 tgctggtcag atttgatttc tccccaggcc acctgatcca tcggaaaacc ttcgtaatat
     6721 tgcaaagtaa acgcatctac cagcgagttg ccgactttca gcggcccgga aacacctggt
     6781 tcttgttgat acttggcgct aaaggtattt ttgccatcca ccagcgaaca ctgttgtttc
     6841 tctttacagg caggggaatc tttatagtta acgatttttt ccagtagttg gtagctgtcg
     6901 gtaagctgga gtttgctgag ctctttctcc attgccgcca ccgcctgttc actgaatgcg
     6961 gcggaatcat cggtgatcac cgggttaaag gttgggtcca tagtgcccat tttttcctgg
     7021 tgatgcacag gaatatcaca ccccgggaat gcgccggtaa taaagaactg tgcggtcgca
     7081 acggtacgtt gcagactatt ggcataggca taaacggtgt ccggcggcgg gcattccccc
     7141 gatttcacca tcccctgctg tgccagccat tcacgcatgt aatggcccat atacacttcg
     7201 agcacgccac ctttggtggt gagttgccca ccggggacgt cccattcggg ccatttattc
     7261 ggcgtagact gctccagcac actgccattg ttcgccagcg gcgcacgtaa gttatggcgg
     7321 ctcatcatga gcacttgctg tagctgatag ccttccggta cggtttgtgc ctgagcattt
     7381 gaagcgagta aaactatccc tgccacagtt gcggcgatta gcgttttgtt cattcctggc
     7441 acctcttttg ttatcaatca ctaaagagtg tgacagaaat atgacatttt tccgggaaca
     7501 ggttcgcaaa atggcttgtt gaatgcctgt ttttctatat acatctttaa aaacaggcaa
     7561 ggtttgacgc aaactatgcc tgattagctc cctgcctcgc gtacgccgct gatgattaca
     7621 cctgtggcta aacagaatat cagcggcgtt actaatacag aggcaatagt accaataata
     7681 agagaagtga ggctaataac aatggttttt agcacatgcc tttgtcagta aattgcataa
     7741 cgagttaaaa aatcaatttg cgcattcctt tccttattgt ttaactcgta agcctgagca
     7801 aaaagttcag gagggaggta aacgtcataa tgattgccag tgtttattaa atacccatac
     7861 tcaaggtcgt catctgttga aaaggattca ttccctaagg agattgatgc cacattacca
     7921 ttaacaataa ataatgagca aatgctatct gaaagagcag tgaggaaatt tttcacctca
     7981 ttaaccttct gctctgggtc ggtgtctaaa acaaggcgtg ccttattatc attaaatata
     8041 aactcgggta aatgcagtat gcattttttg tcattc
//
LOCUS       DAZGLJ010000197         7982 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000197 DAZGLJ010000000
VERSION     DAZGLJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.197"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            115..726
                     /gene="ruvA"
                     /locus_tag="ACHK49_003908"
     CDS             115..726
                     /gene="ruvA"
                     /locus_tag="ACHK49_003908"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO9264463.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPTTDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            735..1745
                     /gene="ruvB"
                     /locus_tag="ACHK49_003909"
     CDS             735..1745
                     /gene="ruvB"
                     /locus_tag="ACHK49_003909"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO9264464.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1892..2677)
                     /gene="znuB"
                     /locus_tag="ACHK49_003910"
     CDS             complement(1892..2677)
                     /gene="znuB"
                     /locus_tag="ACHK49_003910"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO9264465.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(2674..3429)
                     /gene="znuC"
                     /locus_tag="ACHK49_003911"
     CDS             complement(2674..3429)
                     /gene="znuC"
                     /locus_tag="ACHK49_003911"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO9264466.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3508..4440
                     /gene="znuA"
                     /locus_tag="ACHK49_003912"
     CDS             3508..4440
                     /gene="znuA"
                     /locus_tag="ACHK49_003912"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO9264467.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4456..5778
                     /gene="mepM"
                     /locus_tag="ACHK49_003913"
     CDS             4456..5778
                     /gene="mepM"
                     /locus_tag="ACHK49_003913"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO9264468.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQATQEDEAIPLDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5898..6869
                     /gene="lpxM"
                     /locus_tag="ACHK49_003914"
                     /gene_synonym="msbB"
     CDS             5898..6869
                     /gene="lpxM"
                     /locus_tag="ACHK49_003914"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO9264469.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(6958..>7982)
                     /locus_tag="ACHK49_003915"
     CDS             complement(6958..>7982)
                     /locus_tag="ACHK49_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HFO9264470.1"
                     /translation="DLISLYDKRYGKEYITENMHSEIRAYHEDAGFFAAWDFASNYRD
                     GESYVLLAEKMTTVISGPKTTMTLIYHYNSSYLRFAFTLTQDSPRVDVQTFIDWHEPN
                     VSLKVKFPVSVQTSLAQCQIQFGVIDRPTHSDDSFAFAKDEIPAHHWVDLSDQETGIA
                     LIAKNKYGYRVKDQTLELTLLRSQHKPGEVVKTDNYDNFLINNFADIGKQKFTFSLFP
                     HHGDYRVGGVVNQAYIMNHPLQIVPVKPHPGELPPSLSFFAIDTNSVIIETIKLAEDG
                     DGIIVRLYESYGLEISAMFSWVCNYHYVQYVDLQENKLDDSFYCNQYCNLEFKPFEII
                     TLRLTQ"
BASE COUNT         2117 a         1901 c         2023 g         1941 t
ORIGIN      
        1 catttttcag atcgttggga cgcctcgcaa gttttcttca tccttcgctg gatatctatc
       61 cagcattttt ttatcataca gcattatctt tgattcatta cgcaggagcg tcatgtgata
      121 ggcagactca gaggcatcat cattgaaaaa caacccccgc tggtgttaat tgaagtgggc
      181 ggcgtaggct atgaagtgca tatgccgatg acctgttttt atgaactccc tgaagcgggt
      241 caggaagcga tcgttttcac ccactttgtg gtgcgtgaag acgcgcaact gctgtacggt
      301 tttaacaata aacaagagcg cacattgttc aaagagttga tcaaaaccaa cggcgtcggc
      361 ccgaagttgg cgctggcgat cctctccgga atgtcagcgc agcagttcgt taatgccgtt
      421 gagcgtgaag aagtgggggc actggtgaaa ctgccgggta ttggcaaaaa aaccgccgaa
      481 cgcttgattg ttgaaatgaa agaccgattt aaaggtttgc atggcgatct ctttacgcca
      541 gccgccgacc tggtactcac gtcacctgcc agcccgacga ccgacgatgc tgaacaggaa
      601 gcggttgccg cgctggtggc gctgggctat aaaccacaag aagcaagccg catggtgagc
      661 aaaatcgctc gccctgacgc cagcagtgaa acattaattc gcgaagccct acgcgccgcg
      721 ttatgaggta aaggatgatt gaagcagacc gtctgatttc tgccggtacc actttgccgg
      781 aagatgtggc agatcgcgcc attcgcccta aattactgga agagtatgtt ggtcagccgc
      841 aggttcgttc acagatggag attttcatca aagcagcgaa actgcgcggc gatgccctcg
      901 atcatctgtt gattttcggt cctccggggt tgggtaaaac tacgcttgcc aacattgtcg
      961 ccaatgaaat gggcgttaat ttacgcacga cttctggtcc ggtgctggaa aaggcgggcg
     1021 atctggcagc gatgctcact aaccttgaac cgcatgacgt gttgtttatt gatgagatcc
     1081 accgtctttc gccagtggtg gaagaggtgt tgtatccggc aatggaagac taccaactgg
     1141 atatcatgat tggtgaaggt ccggcggcac gctccattaa aattgatttg ccgccgttta
     1201 ccctgattgg tgcaaccacg cgcgcaggtt cgctgacatc accgttgcgc gatcgttttg
     1261 gtattgtgca gcgtctggag ttttatcagg tgccggatct gcaatatatc gtcagtcgca
     1321 gcgcacgctt tatggggctt gagatgagtg atgacggcgc gctggaagtt gctcgtcgag
     1381 ctcgcggtac gccgcgcatt gccaaccgtc tgctgcgtcg agtgcgtgat ttcgccgaag
     1441 tgaagcacga tggcaccatc tcggcagata tcgctgctca ggcgttggat atgttgaatg
     1501 tcgatgctga aggtttcgat tatatggacc gcaagttatt gctggcggta atcgataagt
     1561 tctttggtgg gccggtaggt ctggataacc ttgcggcagc cattggcgaa gaacgtgaaa
     1621 ccattgagga tgtgctggaa ccttatttga ttcagcaagg ctttttgcag cgtacaccgc
     1681 gtgggcgtat ggcgacgacg cgggcgtgga atcactttgg cataacgccg ccagaaatgc
     1741 cgtaagtcgg attggtcgct cgcgccgcat ccgacactgg cagtgtgtct gatgcgacac
     1801 tgttcgcgtc ttatcaggcc agccagagat agcgaaccgt aggtcggata aggcgctcgc
     1861 gccgcatccg acaaatgtgt tcagcgatag attagctggc ctgctttttc atcatactga
     1921 gaataaataa cagtgccgca catagcacca ccgacggacc cgccggcgta tcgtaaaacg
     1981 cggaaaaggt taaaccgcca gttactgcca ccatccccac caaaacagcg acaccagcca
     2041 tctgttccgg cgtacgggca aagcgacgcg cagtagctgc aggaataatc agcagtgaag
     2101 taataatcaa cgcgccaaca aatttcatcg ctacaccaat cgtcaatgcc gtcaccagca
     2161 tcaacaacaa tttcacgcgc tgtaatttca caccatcaac aaacgccaga tccgggctaa
     2221 tcgtcatcga cagcaaattg cgccattgcc agaacaaaat agccaccacg atgaccacgc
     2281 caatcgcaat agcgatgaga tcttctggcg tcactgccag caaatcaccg aacaggtaag
     2341 ccatcaaatc aacacgaatg ttagacatca gactaacgac caccaggccc agcgacaggg
     2401 cactgtgcgc cataatccct aataacgtgt caatcgccag ctgtggacgc ttctccagcc
     2461 ataccagacc gcccgccagg agcagcgtaa cggcaattac cgcatagaat ggattcacgt
     2521 ccagcaacaa accaaacgcg acgccaagta atgaggcatg agccagcgta tcaccgaaat
     2581 aagacatacg acgccagact acaaacgaac ccagcggacc cgcggcacag gcgagcatga
     2641 tcccggctaa ccaaccggga aataataatt caatcatgag cgatcatttc cccgacgcaa
     2701 aacaattcgt ccctgtaaat cgtgacgatg attatgatga tggcgataga tacccagttg
     2761 ttcagcacca cgaggaccaa acatagaaat aaactccgga tgtagggaaa caacttccgg
     2821 tgtgccggaa caacaaatgt ggtgattcag acacagcact tcatcggttt ttgccattac
     2881 cagatgcaga tcgtgagaga ccattaaaac gccacaatcc agttcgcgac gcaactggtc
     2941 aataaggtca tataacgcca cctggccatt tacatccacg ccttgagtgg gttcatccag
     3001 cactaataat tgcggtcgat ttaacaatgc tcgcgctaac agtacacgct gtgtttcgcc
     3061 accagagagc ttttgcatcg gtgcgttaat cagatgcccg gcctggacac gtttcagtgc
     3121 aggcaaaata tcttctttat gtgtgccagg gcgtaagcgt aaaaaacggt ttacggtcag
     3181 tggcaacgtg gtgtcgagat acagcttctg cggtacatag ccgatgcgta gttttccgtt
     3241 gcgcttgata accccttcat cgggtgttac cagcccgagc actacccgta ccagtgtcga
     3301 cttacctgcg ccatttggcc caagtaaagt caaaattttt ccaggtttaa gttccagcga
     3361 cacatcagag aggacgcggc gttggccaaa agaaaccgag acattttcca gggaaaccag
     3421 acttgtcatg ttaattttag tcttgcagta gtcatgaaat gttataatat cacacttctc
     3481 atattcatta cgatgattgg tcgcattatg ttacataaaa aaacgcttct tttcgcagca
     3541 ttatccgccg ctctctgggg aggtgcaaca caggccgcag atgccgccgt tgtcgcttcg
     3601 cttaaacccg ttgggttcat cgcttctgcc attgctgatg gtgtaacaga aacggaggtt
     3661 ttactgcctg acggcgcttc agaacatgat tattcactgc gtccatcgga tgtaaaacgc
     3721 ttacagaacg cggacttagt cgtttgggtt ggcccggaga tggaagcgtt tatgcaaaaa
     3781 ccggtaagca aattaccagg agcgaagcag gtaacgattg cgcagcttga agatgtgaaa
     3841 ccgctactga tgaaaagtat tcacggcgat gatgatgatc acgaccacgc ggaaaaaagt
     3901 gacgaagatc accatcacgg cgatttcaac atgcatcttt ggctttcccc agagatagcg
     3961 cgggctacag cggttgcaat ccatggaaaa ttagtggaac ttatgccgca aagtcgagcc
     4021 aaacttgacg ccaacctgaa ggattttgag gcacaattag cctcaaccga aacgcaggtt
     4081 ggtaacgagc tcgcgccgct caaggggaaa ggttatttcg tttttcacga tgcttacggc
     4141 tatttcgaaa aacagtttgg actgacaccg cttggtcatt ttaccgttaa ccctgagatt
     4201 caacctggcg cgcagcgttt acatgaaata agaacacagt tggttgagca aaaagcaacc
     4261 tgcgtttttg ctgagccaca gttcaggcca gcggtcgttg agagcgtcgc acgagggaca
     4321 tccgttcgta tgggaacgtt ggatcccctg gggacgaata tcaaactggg taaaacaagc
     4381 tattcagaat tcctgagtca attagccaac cagtatgcga gctgcctgaa aggagattaa
     4441 tgaggaagtg attacgtgca acagatagcc cgctctgtcg ccctggcgtt taataattta
     4501 ccgcgaccac accgcgttat gttggggtcg ctcaccgttc ttactctggc cgtcgctgtc
     4561 tggcggcctt atgtttatca ccgtgatgcc acgccaattg tcaaaaccat tgagctggaa
     4621 cagaacgaaa ttcgttcgct cttacctgaa gccagtgagc cgattgatca agctacacaa
     4681 gaagatgaag ccattcccct ggacgaactg gatgacaaaa tcgccggtga agcgggcgtg
     4741 catgaatatg ttgtttccac tggcgatacg ctaagcagca ttctcaatca gtatggtatt
     4801 gatatgggtg atatcaccca actggctgcg gccgacaaag aattgcgtaa cctgaaaatc
     4861 ggtcaacaac tctcctggac attaaccgcg gacggcgaac tgcagcgcct cacctgggaa
     4921 gtgtctcgtc gtgaaacccg aacctatgac cgtactgccg ctaacggttt taaaatgacc
     4981 agcgaaatgc agcaaggaga gtgggttaac aatctgctga aaggtaccgt cggggggagc
     5041 tttgttgcca gcgccagaaa cgccggttta accagcgccg aagtgagcgc agtgattaaa
     5101 gccatgcagt ggcaaatgga tttccgcaaa ctgaaaaaag gcgatgaatt tgcggtgtta
     5161 atgtctcgag aaatgcttga tggtaaacgt gagcaaagcc agctgctggg cgtacgtttg
     5221 cgttcagaag gtaaagatta ttacgctatc cgcgctgaag atggcaaatt ctacgatcgt
     5281 aacggtactg gtctggcgaa aggattcttg cgattcccga cggcgaaaca gttccgtatc
     5341 tcgtctaact ttaacccgcg tcgaactaat ccggtgacag gtcgcgttgc accacacaga
     5401 ggtgttgatt tcgccatgcc acaaggtacg ccagtgcttt cagtgggtga cggtgaagtg
     5461 gtggttgcca aacgcagtgg cgcagcaggt tattatgtgg ctattcgtca tggtcgcagc
     5521 tacaccacgc gttatatgca cttgcgcaag attctggtga aaccgggaca gaaggtgaaa
     5581 cgtggcgacc gtatcgcgct ttccggtaat accggacgtt caactgggcc gcatctgcac
     5641 tatgaagtat ggataaacca gcaggccgta aacccgctga cggcaaaact gccgcgtact
     5701 gaagggctga ccggctccga tcgtcgcgaa ttcctggcgc aggccaaaga gattgtgccg
     5761 cagctacggt ttgattaatt aacatccatt cgcagccggt acgcagtcag taccggcttt
     5821 ttttatttgg tgcggggcaa gttgcgccgc tacactatca ccagattgat ttttgcctta
     5881 tccgaaactg gaaaagcatg gaaacgaaaa aaaataatag cgaatacatt cctgagtttg
     5941 ataaatcctt tcgccacccg cgctactggg gagcatggct gggtgtagca gcgatggcgg
     6001 gtatcgcttt aacgccgcca aagttccgtg atcccattct ggcacggctg ggacgttttg
     6061 ccggacgact gggaaaaagc tcacgccgtc gtgcgttaat caatctgtcg ctctgctttc
     6121 cagaacgtag tgaagctgaa cgcgaagcga ttgttgatga gatgtttgcc accgcgccgc
     6181 aagcgatggc aatgatggct gagttggcaa tacgcggacc ggagaaaatt cagccgcgcg
     6241 ttgactggca agggctggag atcatcgaag agatgcggcg taataacgag aaagttatct
     6301 ttctggtgcc gcacggttgg gccgtcgata ttcctgccat gctgatggcc tcgcaagggc
     6361 agaaaatggc agcgatgttc cataatcagg gcaacccggt ttttgattat gtctggaaca
     6421 cggtgcgtcg tcgctttggc ggtcgtctgc atgcgagaaa tgacggtatt aaaccattca
     6481 tccagtcggt acgtcagggg tactggggat attatttacc cgatcaggat catggcccag
     6541 agcacagcga atttgtggat ttctttgcca cctataaagc gacgttgccc gcgattggtc
     6601 gtttgatgaa agtgtgccgt gcgcgcgttg taccgctgtt tccgatttat gatggcaaga
     6661 cgcatcgtct gacgattcag gtgcgcccac cgatggatga tctgttagag gcggatgatc
     6721 atacgattgc gcggcggatg aatgaagaag tcgagatttt tgttggtccg cgaccagaac
     6781 aatacacctg gatactaaaa ttgctgaaaa ctcgcaaacc gggcgaaatc cagccgtata
     6841 agcgcaaaga tctttatccc atcaaataaa aaacagcctc tcaaattatt gagaggctgt
     6901 acttgataaa aaatctcaat gagtgttctc accattttga gtttttagat tagtacgcta
     6961 ttgagttaat cgtagcgtga taatttcaaa aggtttgaat tcaagattgc aatattgatt
     7021 gcaataaaaa ctgtcatcta atttattttc ttggagatcg acatattgaa cgtagtgata
     7081 attacatacc cacgaaaaca tggctgaaat ttctaatcca taactctcat ataatcttac
     7141 gattataccg tcgccgtctt cagctaattt tattgtttcg atgataacgc tgttagtatc
     7201 gatagcaaaa aatgatagtg aagggggtag ttcacccggg tgtggtttga cagggacgat
     7261 ctgtaaggga tgattcatta tataggcctg gttaacgaca ccacccacac ggtaatctcc
     7321 atgatgagga aataaggaga atgtgaattt ttgtttacca atatctgcaa aattattaat
     7381 caggaaatta tcataattat ctgtcttaac aacttcccca ggtttgtgct gcgaacgcaa
     7441 caaagttaac tctagcgtct ggtctttgac acgatagcca tatttgtttt ttgctattaa
     7501 agcaatgcca gtttcttgat cagaaaggtc gacccagtga tgtgctggta tttcatcttt
     7561 ggcaaaagcg aacgaatcat cgctatgcgt tggtctgtca attacaccga actgaatttg
     7621 acattgtgca agagatgttt gtactgatac cgggaatttt actttgagag aaacattagg
     7681 ttcgtgccaa tcaataaaag tttggacatc aacgcgtgga ctgtcctgtg tcagggtaaa
     7741 tgcgaagcga agataagagg aattgtagtg ataaatcagc gtcatcgtag ttttaggccc
     7801 gctgatcaca gttgtcattt tttcagcgag taaaacatat gattccccat cacgataatt
     7861 tgaagcaaaa tcccaggccg cgaaaaaacc tgcatcttcg tggtatgcac ggatctcact
     7921 gtgcatattt tcagtaatgt attctttacc atatctttta tcatatagcg aaattaaatc
     7981 ac
//
LOCUS       DAZGLJ010000198         7959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000198 DAZGLJ010000000
VERSION     DAZGLJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.198"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            107..196
                     /gene="kdpF"
                     /locus_tag="ACHK49_003916"
     CDS             107..196
                     /gene="kdpF"
                     /locus_tag="ACHK49_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO9264471.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            196..1869
                     /gene="kdpA"
                     /locus_tag="ACHK49_003917"
     CDS             196..1869
                     /gene="kdpA"
                     /locus_tag="ACHK49_003917"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO9264472.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLDQHYLPLNPQQLPGLSWDLA
                     LNAAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGSVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1892..3940
                     /gene="kdpB"
                     /locus_tag="ACHK49_003918"
     CDS             1892..3940
                     /gene="kdpB"
                     /locus_tag="ACHK49_003918"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO9264473.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSARGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3949..4521
                     /gene="kdpC"
                     /locus_tag="ACHK49_003919"
     CDS             3949..4521
                     /gene="kdpC"
                     /locus_tag="ACHK49_003919"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO9264474.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4514..7198
                     /gene="kdpD"
                     /locus_tag="ACHK49_003920"
     CDS             4514..7198
                     /gene="kdpD"
                     /locus_tag="ACHK49_003920"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO9264475.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLTDPAEEKAVVRYARE
                     HNLGKIILGRPASCRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7195..7872
                     /gene="kdpE"
                     /locus_tag="ACHK49_003921"
     CDS             7195..7872
                     /gene="kdpE"
                     /locus_tag="ACHK49_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO9264476.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
BASE COUNT         1627 a         2072 c         2362 g         1898 t
ORIGIN      
        1 tgccattttt atactttttt tacaccccgc ccgcagattt ttgcgaaatc tttgcagcca
       61 gaattctacc cttccggtat cacttttagg ccactggagg tgcactgtga gtgcaggcgt
      121 gataaccggc gtattgctgg tgtttttatt actgggttat ctggtttatg ccctgatcaa
      181 tgcggaggcg ttctgatggc tgcgcaaggg ttcttactga tcgccacgtt tttactggtg
      241 ttaatggtgc tggcgcgtcc tttaggcagc gggctggcgc ggctgattaa tgacattcct
      301 cttccaggta cagcgggcgt tgaacgtata ctctttcgtt tacctggcgt ctctgaccat
      361 gagatgaact ggaagcaata tctttgtgcc attctcggcc tgaacatgct ggggctggcg
      421 gtgctgtttt ttatgttgct cgatcagcac tatctgccgc ttaatccaca gcagttgccg
      481 gggctgtcgt gggatctggc gctgaatgcc gccgtcagct ttgtcacgaa taccaactgg
      541 caatcttaca gcggtgaaac cacgttgagc tatttcagcc agatggctgg cttaacggtg
      601 caaaactttc tttctgccgc cagcgggatt gcggtgattt ttgccctcat ccgtgcgttt
      661 acccgccaga gcatgagcac actcgggaat gcctgggtcg atctgctacg catcacgtta
      721 tgggtgctcg tccctgtggc gttgttgatc gcactgtttt ttattcaaca aggtgcgctg
      781 caaaactttc agccttatca ggctgtgaat accgttgaag gagcgcaaca gctgttaccc
      841 atggggcctg tagcttctca ggaagcgatc aagatgctcg gtactaacgg cggtggcttc
      901 tttaatgcca actcgtcgca tccgtttgaa aacccaaccg cactgaccaa cttcgtgcag
      961 atgctggcga tcttcttgat cccaacggcg ctgtgctttg cctttggtga agtgacgggc
     1021 gatcgccgcc aggggcgcat gttgctgtgg gcgatgtcag tgatttttgt catctgcgta
     1081 ggcgtggtga tgtgggcaga agttcagggt aatcctcatc tgctggcgct cggcgcagac
     1141 agcagcatca atatggaagg taaagagagc cgtttcggcg tactggtcag tagcctgttt
     1201 gcggtcgtga ctacggcggc ttcctgtggc gcggtgattg cgatgcatga ttcgtttacc
     1261 gctctcggtg gcatggtgcc gatgtggctg atgcaaattg gtgaagtggt gttcggcagt
     1321 gtcggttctg gtctttacgg catgatgctg tttgtcctgc tggcggtgtt tattgccggg
     1381 ctgatgattg gtcgtacacc ggaatatctg ggtaaaaaaa tcgacgtacg cgagatgaaa
     1441 ctgactgcac tggcgattct ggttaccccg acgctggtgc tgatgggcgc ggcgctggca
     1501 atgatgaccg acgccggacg tagcgccatg ctcaaccctg gcccgcatgg ttttagcgaa
     1561 gtgctgtacg ccgtgtcatc cgccgctaac aacaacggca gcgcctttgc cggattaagc
     1621 gccaactccc cgttctggaa ctgtttactg gcgttctgca tgtttgtcgg tcgcttcggg
     1681 gtgattatcc cggtgatggc aattgccggt tcgctggtga gtaaaaagag ccaacccgcc
     1741 agctccggca cgctgccaac gcacggcccg ctgtttgttg gcctgttaat cggcaccgtg
     1801 ttgctggttg gcgcactgac ctttatccct gccctggcgc ttggtccggt ggcggaatat
     1861 ctctcctgat gatattgagt gagcactgaa tatgagtcgt aaacaactgg cgctattcga
     1921 accaacactt gtcgttcagg cgctgaaaga agcggtgaaa aaattaaacc cgcaggcgca
     1981 atggcgcaat ccggtgatgt ttatcgtctg gatcggcagt ctgctgacca cctgtattag
     2041 catcgcgatg gcaagcggtg cgatgcctgg caatgcgctg tttagcgcgg caattagcgg
     2101 ctggctgtgg gtcaccgtac tgttcgctaa tttcgccgag gcgctggcag aaggccgcag
     2161 taaagcgcag gccaacagtc ttaaaggggt gaaaaaaact gcctttgccc gcaagctgcg
     2221 tgagccgaaa tatggcgctg cggcggacaa agttcctgcc gatcaactgc gtaaaggtga
     2281 catcgtgctg gtagaagctg gcgatattat cccctgcgat ggtgaagtca ttgaaggcgg
     2341 cgcatcggtc gatgaaagcg ccatcaccgg ggaatcggca ccggtgatcc gtgaatccgg
     2401 cggcgatttt gcctccgtca ccggcggcac gcgtattctt tctgactggc tggtgattga
     2461 gtgtagcgtt aaccccggtg agacatttct ggatcggatg atcgcgatgg tggaaggcgc
     2521 acagcgacgc aaaacaccga acgagattgc cctgactatc ctgctgattg ctctgaccat
     2581 cgtcttttta ctggcaaccg ccacgctgtg gccgttttcc gcgaggggcg gtaatgccgt
     2641 gagtgtaacg gtactggtgg cgctgctggt ctgtctgatc ccaaccacta ttggcggcct
     2701 gttgtcagcg atcggcgtcg ccgggatgag tcggatgctg ggcgcgaatg tgattgccac
     2761 cagcggacgt gcagttgaag cggcaggtga cgttgacgtt ctgctactgg ataaaaccgg
     2821 caccatcaca ctcggtaacc gtcaggcgtc ggagtttatc cctgcgcagg gcgtggatga
     2881 aaaaacgctg gctgacgccg cacaactggc ttcgctggct gatgaaacgc cggaaggccg
     2941 cagtattgtg atcctcgcca agcaacgttt taacctgcgc gagcgcgatg tgcagtcgct
     3001 ccatgccacc tttgtaccgt ttactgcgca aagccggatg agcgggatca acatcgacaa
     3061 ccgcatgatc cgtaaaggtt ctgtcgatgc cattcgccgc catgttgagg ctaacggcgg
     3121 tcacttccct accgatgttg atcaaaaagt cgatcaggtt gcgcgtcagg gggccacgcc
     3181 gctggtggtg gtggaaggtt cccgtgtgct gggcgttatt gcgctgaaag atatcgtcaa
     3241 aggcggtatt aaagagcgct tcgcccagct gcgcaaaatg ggcattaaaa cggtgatgat
     3301 taccggcgat aaccgtctga ctgccgccgc cattgctgcg gaagcgggcg tcgatgattt
     3361 tctcgccgaa gcgacaccgg aggccaagct ggcattgatt cgtcagtatc aggcggaagg
     3421 ccgtttggtg gcgatgaccg gcgacggcac caacgatgct ccagcgctgg cgcaggcaga
     3481 tgtcgcggtg gcgatgaact ccggtactca ggcggcgaaa gaagcgggca atatggtcga
     3541 tctcgactct aacccgacca agttgatcga ggtggtacat attggcaaac agatgctgat
     3601 gacccgtggc tcgctgacca ccttcagcat tgccaacgat gtggcgaaat acttcgccat
     3661 tattccggcg gcgttcgcgg caacgtatcc gcagttaaat gcgctgaaca tcatgcgcct
     3721 tcattcgccc gactccgcca ttctcagtgc ggtcattttc aacgccttga ttatcgtctt
     3781 tttgattcct ctggcgttaa aaggcgtgag ttataaaccg cttaccgctt ctgccatgtt
     3841 gcgccgtaac ttatggattt acggtctggg tgggctgctg gtgccgttta ttggtatcaa
     3901 agtcattgat ttactgctga ccgtttgcgg tctggtgtga ggtttaccat gcgtggctta
     3961 cgtccggcat tatcgacatt tatctttctg ttattgatta ctggcggcgt ttacccgctg
     4021 ctgaccaccg tgctggggca atggtggttt ccctggcagg ccaacggttc gttgattcgt
     4081 gaaggtgata cggtgcgggg ttcggcatta atcgggcaga attttaccgg caacggctat
     4141 tttcagggtc gcccgtcggc aacggcagaa atgccctata atccacaggc ttctggcggg
     4201 agcaatctgg cggtcagtaa ccctgagctg gataaacaaa tagccgcacg cgttgctgca
     4261 ttacgggccg ctaacccgga tgccagtacg aacgttccgg ttgaactggt gacggcatcg
     4321 gcaagcgggc tggacaataa tatcaccccg caagcggcgg tctggcaaat cccacgcgtg
     4381 gcgaaagcgc gtaatctcag cgttgaacag ctcacgcaac tgatcgcaaa atacagccaa
     4441 caaccgctgg tgaaatatat cggccagccg gtcgtcaaca ttgttgaact caatctggcg
     4501 ctggataaac ttgatgaata acgaaccctt acgtcccgac cccgatcgtc tgctggaaca
     4561 aactgccgcg ccgcatcggg ggaagctgaa agttttcttc ggtgcctgtg caggcgtcgg
     4621 gaagacctgg gcgatgctgg cagaagccca gcgactgcgg gcgcaagggc tggatattgt
     4681 ggttggcgtg gtagaaaccc atgggcggaa agataccgcc gccatgcttg aagggctggc
     4741 tgttctgccg cccaaacgcc aggcgtaccg tgggcggcat atcagcgagt ttgatctcga
     4801 tgccgccctc gcccgccgtc cggcgctgat cttaatggac gaactggcgc acagtaatgc
     4861 gccaggttct cgtcatccta aacgctggca ggatatcgaa gaactgctgg aagctggcat
     4921 tgatgttttc acaaccgtca acgttcagca tctggaaagt ctgaatgatg tggtcagcgg
     4981 cgtcaccgga attcaggtac gggaaaccgt gcccgatcct tttttcgatg ccgccgacga
     5041 cgtagtgctg gtggacttgc ccccggacga tctgcgccag cggctgaaag aaggcaaagt
     5101 ctatattgcc gggcaggcgg agcgcgccat tgaacatttt ttccgcaaag gcaatctgat
     5161 cgccctgcgt gaactggcac tgcgccgtac tgccgatcgc gtagatgagc aaatgcgcgc
     5221 ctggcggggg catcctggtg aagagaaagt gtggcacacg cgcgacgcga tccttttatg
     5281 catcgggcat aacaccggca gtgagaaact ggtccgcgcg gcggcgcggt tggcctcgcg
     5341 cctgggtagc gtctggcacg cggtgtatgt tgaaacccct gccctgcatc gcttaccgga
     5401 aaaaaaacgt cgggcaattc tcagcgcctt acgtctggcg caggagctgg gcgcggagac
     5461 ggctacactt actgatccag cggaagaaaa agcagtagtg cgttatgccc gtgaacataa
     5521 tctcggcaag attattctcg gtcgcccggc ctcgtgccgc tggtggcgtc gggaaacgtt
     5581 tgctgaccga ctggcgcgca tcgcccccga tctcgatcag gtgctggtcg cgcttgatga
     5641 accacccgcc cgcacgatta acaacgcgcc ggatagccgc tcttttaaag acaagtggcg
     5701 ggtacagatt cagggatgcg tggttgccgc cgcgttatgc gccgttatca ctttaattgc
     5761 catgcagtgg ctgatggcgt ttgatgccgc caacctggtg atgctgtatc tgcttggcgt
     5821 ggtggtggtg gcgctatttt atggacgctg gccttcggtg gttgctaccg tcattaatgt
     5881 ggtgagtttc gatctctttt ttatcgcccc acgcggcacg ctcgctgtct ctgatgtgca
     5941 atatctgctg accttcgcgg tgatgttaac cgtcgggctg gtgatcggga accttactgc
     6001 gggtgtgcgt tatcaggcgc gggtagcccg ttaccgcgag caacgcacac ggcacttata
     6061 tgaaatgtcg aaagctctgg cggtgggccg cagtccgcag gacatcgccg ccaccagcga
     6121 acaatttatt gcctccacgt ttcatgcccg cagtcaggtg ttgttgcccg atgacaacgg
     6181 taaattgcag ccgttaacac atccgcaagg aatgacgcca tgggacgatg ccatcgcgca
     6241 gtggagtttt gataaaggcc tgcctgcggg tgcgggcacc gacacgttac ccggcgtgcc
     6301 gtaccagatt ttgccgctaa aaagcggcga gaaaacctac ggactggtgg tggtggaacc
     6361 ggggaatctg cgtcagttga tgatcccgga gcagcagcgc ctgctagaga cgtttacgct
     6421 gttagtcgcc aatgcgtttg agcggctgac gctgaccgcc agtgaagaac aggcgcggat
     6481 ggcaagcgaa cgtgaacaga tccgcaacgc cctgctggcg gcgctttcgc atgatttacg
     6541 cacgccgctt acggtgctgt ttggtcaggc agaaatctta acgctcgatc tggcaagcga
     6601 aggatcgccc cacgcccgcc aggccagcga gattcgtcag catgtgctga acactacccg
     6661 actggtgaat aatctactgg atatggcgcg aattcagtcc ggcggcttta atttgaagaa
     6721 agagtggtta acgctggaag aagtagtcgg cagcgctctg caaatgctgg aaccgggttt
     6781 atcgtcgccc atcaatcttt ctctgccaga accgctgacc ttaatccacg ttgacgggcc
     6841 actctttgaa cgggtgctga ttaatctgct ggagaacgcg gtgaaatatg cgggtgcgca
     6901 ggccgaaatt ggtatcgatg cccacgttga gggcgaaaat ctacaactgg atgtctggga
     6961 taacggcccc ggtcttccgc caggccagga gcagacgata tttgataagt ttgctcgcgg
     7021 gaataaagag tcggcagtac cgggggtagg gcttggactg gcaatttgtc gggcgatagt
     7081 ggatgtacac gggggcacta ttaccgcgtt caaccgaccg gaaggtggtg cctgttttcg
     7141 tgttacactt ccccagcaaa ccgctcctga acttgaagac tttcatgagg atatgtgaca
     7201 aacgttctga ttgttgaaga tgaacaggct attcgtcgct ttctgcgcac ggcgctggag
     7261 ggcgacggga tgcgcgtcta tgaggccgaa acgctgcaac gcggcttgct ggaagcggct
     7321 acccgtaagc cagatttgat tattctcgat ctcggcctgc ccgatggtga tgggattgag
     7381 tttatccgcg acctgcgcca gtggagcgcg gtgccggtga ttgtgctttc cgcacgcagc
     7441 gaagagagcg acaaaatcgc cgcgctggat gccggagcgg atgattatct gagtaagccg
     7501 tttggcattg gcgaattgca ggcccgtctg cgcgtcgctt tacgccgcca ctccgccacc
     7561 gccgcgcccg atccgctggt gaaattttcc gatgttaccg tcgatttagc cgcccgcgtg
     7621 attcaccggg gtgaggaaga ggtgcatctc acgccgattg agttccgcct gctggcggtg
     7681 ctgctaaata atgccgggaa agtgctcacc cagcgccagt tactcaatca ggtgtggggg
     7741 ccaaacgcgg tcgaacacag tcactatttg cgtatttata tgggacatct gcgacaaaaa
     7801 ctggaacagg atcccgctcg cccccgccat ttcattactg aaaccggtat tgggtatcgg
     7861 tttatgccat gaatgtttat tcaataaatg cgcttaaata attattaatt tattattaaa
     7921 cctgccaaaa atattattat ttggcaggtt taatttctt
//
LOCUS       DAZGLJ010000199         7928 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000199 DAZGLJ010000000
VERSION     DAZGLJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.199"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            83..198
                     /gene="rrf"
                     /locus_tag="ACHK49_003922"
     rRNA            83..198
                     /gene="rrf"
                     /locus_tag="ACHK49_003922"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            521..1819
                     /gene="kgtP"
                     /locus_tag="ACHK49_003923"
     CDS             521..1819
                     /gene="kgtP"
                     /locus_tag="ACHK49_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO9264477.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1816..2139)
                     /gene="yfiM"
                     /locus_tag="ACHK49_003924"
     CDS             complement(1816..2139)
                     /gene="yfiM"
                     /locus_tag="ACHK49_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO9264478.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(2185..3540)
                     /gene="pssA"
                     /locus_tag="ACHK49_003925"
     CDS             complement(2185..3540)
                     /gene="pssA"
                     /locus_tag="ACHK49_003925"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO9264479.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3654..6314)
                     /gene="pat"
                     /locus_tag="ACHK49_003926"
     CDS             complement(3654..6314)
                     /gene="pat"
                     /locus_tag="ACHK49_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO9264480.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6346..7044)
                     /gene="tapT"
                     /locus_tag="ACHK49_003927"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6346..7044)
                     /gene="tapT"
                     /locus_tag="ACHK49_003927"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO9264481.1"
                     /translation="MTENGVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMASDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(7113..7532)
                     /gene="trxC"
                     /locus_tag="ACHK49_003928"
     CDS             complement(7113..7532)
                     /gene="trxC"
                     /locus_tag="ACHK49_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO9264482.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7739..>7928
                     /locus_tag="ACHK49_003929"
     CDS             7739..>7928
                     /locus_tag="ACHK49_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase YfiF"
                     /protein_id="HFO9264483.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRK"
BASE COUNT         1926 a         2022 c         2041 g         1939 t
ORIGIN      
        1 tgttttggcg gatgagagaa agattttcag cctgatacag attaaatcag aacgcagaag
       61 cggtctgata aaacagaatt tgcctggcgg ccgtagcgcg gtggtcccac ctgaccccat
      121 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag
      181 agtagggaac tgccaggcat caaattaagc gaaaggccat ccgtcaggat ggcctttttg
      241 cgttggtgaa aaaaatgcct gatgcgacgc tgcgcgtctt atactcccac atatgccaga
      301 ttcagcagcg aatacggctt ccccaacttg cccacctcca tacgtgttct cctcaccaga
      361 aatttatcct taagctcctc aataaccatt ttcctgctaa ctaaattcat ggttaaggtt
      421 gcataatgat acgcaacaaa tgtataacat tttctttacc aaaagaaata aacaaaagcg
      481 accgacaaaa gcatcggatt acggcaggag acataatggc atggctgaaa gtactgtaac
      541 ggcagacagc aaactgacaa gtagtgatac acgtcgccgc atttgggcga ttgtgggggc
      601 ctcttcaggt aatctggtcg agtggttcga tttctatgtc tactcgttct gttcactcta
      661 cttcgcccac attttcttcc cttccgggaa cacgacgact caactactac aaacagcagg
      721 tgtttttgct gcgggattcc tgatgcgccc aataggcggt tggctatttg gccgcatagc
      781 cgataaacat ggtcgcaaaa aatcgatgct gttatcggtg tgtatgatgt gtttcggatc
      841 gctggttatc gcctgcctcc caggttatga aactataggt acgtgggctc cggcattatt
      901 gcttctcgct cgtttatttc agggattatc cgttggcgga gaatatggca ccagcgccac
      961 ctatatgagt gaagttgccg ttgaagggcg caaaggtttt tacgcatcat ttcagtatgt
     1021 gacgttgatc ggcggacaac tgctagccct actggttgtc gtggttttac aacacaccat
     1081 ggaagacgct gcactcagag agtggggatg gcgtattcct ttcgcgttag gagctgtgtt
     1141 agctgttgtg gcgttgtggt tacgtcgtca attagatgaa acttcgcaac aagaaacgcg
     1201 cgctttaaaa gaagctggat ctctgaaagg attatggcgc aatcgccgtg cattcatcat
     1261 ggttctcggt tttaccgctg cgggctccct ttgtttctat accttcacca cttatatgca
     1321 gaagtatctg gtaaatactg cgggaatgca tgccaacgtg gcgagtggca ttatgactgc
     1381 cgcattgttt gtattcatgc ttattcaacc actcattggc gcgctgtcgg ataagattgg
     1441 tcgccgtacc tcaatgttat gtttcggttc gctggcagcc atttttaccg ttcctattct
     1501 ctcagcattg caaaacgttt cctcgcctta tgccgctttt ggtctggtga tgtgtgccct
     1561 gctgatagtg agtttttata catcaatcag tggaatactg aaggctgaga tgttcccggc
     1621 acaggttcgc gcattaggcg ttggcctgtc atatgcggtc gctaatgcta tatttggtgg
     1681 ttcggcggag tacgtagcgt tgtcgctgaa atcaatagga atggaaacag ccttcttctg
     1741 gtatgtgacc ttgatggccg tggtggcgtt tctggtttct ttgatgctac atcgcaaagg
     1801 gaaggggatg cgtctttagt gacgggtcag ttgccagacg gtatagccgg tgcttgcacc
     1861 ggcgacatcc caggccaaat ccttccagct ccagccgctc ccttcggggc ggctatccca
     1921 aagctctttt gacgccccca aactgacaga gaacatcaat ccaaacatgg cactgcgatc
     1981 ccggctcatc ccctgatgct gtgaatattc atttccggcg gcggaaagca tcgccgaggc
     2041 gataaagtgt tgagctttat cctgcccgct ccagctatcg ttagccatat ggctgcatcc
     2101 agaaagtaac aatagcgaac agacaaaaag aatacgcaaa acgtggcctc caaaaaacaa
     2161 accccgtaca ggacggggtt gtgattacag gatgcggcta attaatcggt cgatgcggat
     2221 acggcgcaaa cggcggatga gtttacgaac cttcaccgga taatcggcaa tactttgcag
     2281 atcgcgatag tgcttaacga tggtggtatg ctcgcggatc agctccagtt ctttctctcg
     2341 ctgcggcgca agctccagtt gtggatcgtg gatcaaaatg gcgttttcca gatccagacg
     2401 ccaggcgcgc gggttcaggt tattaccggt gatcaacatc cacttatcat caacccacat
     2461 cccttttagg tgataggtgt tgtcgtcatc tttccataac cgaaccacta gctggtcagt
     2521 attgacgtaa tactgcaaac ggctcaggaa acgacgcaga ttgatctcat agagataagg
     2581 caatgcgcca attatcttga aaggttcatc ttccggaatg tagaagtcgt tcgcggtttt
     2641 atcaccaaca ataatttcga cctttttccc ttcgcgcagc aactggataa tattgcgcac
     2701 aaggattgct ggcaggttga agtatggcgt acagatggtt agtttctgct cggcacaagg
     2761 cataagatgg aaaatggtct tgttcaacag actcgatttc cccagcccca ctagcggcgt
     2821 tacggaaagc tgatcgttgt cggcatcgcc ctggaaatga taagcggcat cacgcagctc
     2881 ctggcggaac agacgaatat cgttcttgat ttccgggctt tttggccgat taacatcatc
     2941 cagacgatta acgccgcggc cattcataat attctgtgta acccattcaa acataatgtc
     3001 tgacatctta cggttacgga taagatgata acggtcgtag cgatatttat cgtgctgatg
     3061 caggtaaaca tcgttcaggc tggcaccgct ataaagtacg ctatcgtcga tgataaagcc
     3121 tttaaagtgc agaacaccaa gggcttcacg ggtattgatt ggaacgccat aaaccggaac
     3181 atctacgccc ggattttcct gcgccatgcg gcagtaccag tcagcgttag tgttagatgc
     3241 cgcagcgcca atgcgtccac gttgtgcacg atgccagtcg accagcaccc gcacatccag
     3301 ttccggacgc tgccttttag cttcatacaa cgcgttcaga atgcctttgc caccgtcatc
     3361 ctgttcgaga tacagggcga caatgcaaat gcgctgcttc gcgctggcta ttttttccag
     3421 cagcgtctcc cggaagtcgg cgggagcgta aaagaaatcg acatcatcaa ctgattgaga
     3481 aatcttgggt agttgggcaa ggtgttgttg atgtttatta cgcttaaatt ttgacaacat
     3541 cacagtgcat ttcttctctg ttcattgaag ggtcctctgt gcaatgcaga cgacataagc
     3601 gggcaataat aacaccagtc ccgattaagt ggtcaacatt tccagtacct tactcatgat
     3661 tcctcgcgtt gggcaagatt tagcgtaagc ccaacgatcc cctcttcgag ctggatatca
     3721 acgttaaacc cgagcttgcg ggctagcgcc accatgccac gattgtttgg catcgtaata
     3781 ccattcagac gttgtagtcc gtgatctcgc gtataggtaa tcaacttttc cattaagcgt
     3841 cgacctaagc ctaacccttt gagatccgag cgaaccagca cagcaaattc ggcatcgatg
     3901 ttatcaggat cggaaatcgc acgcgtgacg ccgaggatct cttccgtttg atcaatacgt
     3961 cgtaccgcta caaacgccat ttcccgatcg tagtcgatct gtgtcatgtt ggctaaatct
     4021 tcatgggtaa attcgttgat ctcgctaaag tagcggtaat aaagatcttc tttggtgact
     4081 cgcgaaatga attgctgaag ttgtggctca tcttctggca aaatcgggcg gaacaagcag
     4141 cgttcaccgt ttttcaattc tacccattct tccagctgat gcggataagg gcgcactgcc
     4201 agccgactct cgttatcgcc ttcaaacggc gagatatcca gcgtgacatc cagcgcggta
     4261 aattcactgc cagaagccag caaaggatga atatccagac gctgaatttc cgggcaatcg
     4321 acaatcaagt tggaaacctg caccagaagc tggctcaagc ctgcaacatc caatgggcgt
     4381 agcgcactgc gtgcacgaat ctttttactt ttgatcccct gaataaccag atagcgggcc
     4441 aggttcatgt tcagcggcgg taacgcgacg acggcttgat cttcaggacg ccactccaca
     4501 ccgccttcgc ccagcatgat caacggcccg aaaaccggat cgtgctcaac cacaacccgc
     4561 aactcctgag caccagcacg gttagccata ctttgcacca acaggccgtg gacccgtgcc
     4621 tgcggccagg ccatttttac gcgatcgaaa atggcgtttg ccgcttgctg gacttcattg
     4681 gctgtacgca ggtagagcat gacgccctga acttccgatt tatgtggaat atccggcgaa
     4741 cgcaatttca gcgccaccgg ataaccaatc tgttcggcaa tatgcaccgc ttcggtgcta
     4801 tcgctggcaa tccaggtggg gagcgtgttc atgccatatg cttgcaggat aggctgaact
     4861 tcatgggtat cgagtgacgt tgctccttcg gcaatcgcct gttgcaacag aagatgcgct
     4921 tctgcggtat tggaggtcag attgctgggc aacgccggcg tttcgcgtag ttgcttctga
     4981 ttacgccggt actccaccat atgcataaaa gcagtgatgg ttccttccgg ggtacggtag
     5041 gtcggcagcc cggcttcgct gaataatcgt cgtgcctctt gcgaggaatg ttcgccacac
     5101 cagttcgtca gcagagaaac atacttgctg cggggatgat gctttaccgc ttcaattaat
     5161 acttgcgcgc tttctgttgc gggagcagcg gcgctgggcg aatgaataac catcagcgca
     5221 tcaaaatcct ggctgtggag cagaatgtcc agcgttttaa tatagtgctc actgctggcg
     5281 tcatcgcgca gatcgagcgg gttagagatt gccacatgtc ctggcagtgc atcgcgcagt
     5341 ttctggcagg tttcttcgct tagtgttgcc agcttgccat tgcgtgacca taaggcatca
     5401 agcgccagcg cggcaggcgc agcaccgttg ctgataatca tcaaccggtc gccacgcagc
     5461 gggcgcatat ggctaagggt ttccaccgcc gaaaacagtt cgtgggtgtc ctgtacccgc
     5521 aacaaaccgg cacgctgaat agccgcatcc cacgccggat ccattcctgc cgtggtgttc
     5581 aacagacgtt gagccgccgg gctacgtccg cttttaatca ccagaatcgg tttgttgcga
     5641 gaggcactac gggccgccga aacaaagcgt cgcgcgtcgc ttaactgttc gagatagagc
     5701 aggatggcgc tggttttact gtcgcgtgcc agatagtcaa gcaattcatc aacgtcgata
     5761 tccaggctgt cgccgagcgc aataaagtag gaaaagccca tcttacgctg ttgcgcccag
     5821 tcgaggatgg tgttggagac ggcagccgat tgcgaaataa acgccagctt gccgcgttta
     5881 atcggcacag gcgaaaagct ggcattcaga ccttgccagg gagccagtaa acccagactg
     5941 tttggtccaa gcaggcgcat gttatggcgc agggcgcagg cgcggagatc ttcgtgttgc
     6001 gatgccgggg cggaaagaat aatgcaggtt ttacagcctt tctcgccgag ctcttccaga
     6061 agagcaagat tacggctggc attggtacat aaaaccgcaa ggtcgggtgt aaagggcaag
     6121 ctggcaatat ccggccaggc caacacaccc aacaccgctt tccaggctgg cgtcaccggg
     6181 agtaccggtc cgttaaagcc tcccgccagc aggttacgca tcatcaggta acctgcgcga
     6241 ttgggtttca tcgacgcgcc aattaccgct atcgattttg gtcgcagtag tgcttccagt
     6301 cctcgctgac tcataccggt ctcccgcaga agtgaccgaa tgattttaaa cgctttctaa
     6361 ctgttctgct gtgatgctac ccagatgttg cgtttttcct gccagatagc gtgttttaaa
     6421 gcgggtaaaa tgctcgccta accctgctgc cgccccggta tcgctggcca tatctaacag
     6481 tgcgatggct acctcggcag tacaatattg gcctgcagcc tgggcttcac gcaggcgata
     6541 ggcagaaagc cgggaaagat cgacggaaat gacgggaaga ttatccagat acggactttt
     6601 acgaaacatc ttgcgagctt ccggccaggt accatcgagc atgataaaca gcggtggctt
     6661 accggcaggt ggtgtgaaga tcacttcccg ttgctcatca gcatacgaag cgggaaagac
     6721 caccattggc tgatagtccg ggttttgcac caactccagc aaatcctgcg agggttcggt
     6781 acgcgaccat tgaaacgcaa cggtatcagg caaaatatca gcaatcagac gcccggtatt
     6841 actgggcttc attggctcgg tgtcgaacat cagcaaacag aagcgacttt ttgcttgtgc
     6901 tggggtaatt gtcgaacaga gacataattt ctctggcaaa agacagcgtt ggcagcgacg
     6961 aacgcgatta ccgcgggcaa gaaaaggacg tgttgcgcgc gcaatacgct cggcgcgtaa
     7021 ctggagaaca ccgttttcgg tcataagaga gcgtcgaaaa aacgccattg tcgcagagga
     7081 gaaaacgggg cacaagatgc gccccggtaa gattaaagag attcgttcag ccagctatcg
     7141 aacggcgctt tcggtactgc gccattaagc atgtcgacaa cctgaccgtt tttgaaaatc
     7201 atgatcgtcg gtatactacg aattccaaag cgactgctca attcacgttc agcttcggta
     7261 ttcactttca caaagcgcac tttaccgcta cgctcttgcg cgacatcttc aaaaattggt
     7321 gcgaaattac ggcaggggcc gcaccacggt gcccagaagt cgatcaccac aggtagatca
     7381 tccttcagca atttgtcgag tgtttcaccg gtcgcattaa tcacctctcc gtcaaacaag
     7441 tcgtgaccgc agcgtccgca ttttgccgca tcttcgatcc gatcgtcggg aatgcgattg
     7501 atggcctgac aatgggtaca aacggtattc ataactaacc tcgggatgag taaggagact
     7561 tatcggcaac gacgccggta tgtttctaat atgttacata ttatcgggga cattgttcca
     7621 gacaacaatc tgttttattc aataggtatg atagtcgcag gctttggtac gaaatcatgg
     7681 cgaaccgcgc ggacttcggg taatctgcgc gcttcgcgca gcgctggtgg agaaaagcat
     7741 gaacgatgaa atgaaaggta aaagcggcaa ggtcaaagtg atgtatgtcc gcagtgatga
     7801 tgattctgat aaacgtaccc acaacccgcg taccgggaaa gggggcgggc gtccaggaaa
     7861 atctcgtgct gacggtggcc gtcgccccgc ccgcgatgac aaacagagtc agccccgtga
     7921 ccgcaagt
//
LOCUS       DAZGLJ010000200         7798 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000200 DAZGLJ010000000
VERSION     DAZGLJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.200"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..887)
                     /locus_tag="ACHK49_003930"
     CDS             complement(<1..887)
                     /locus_tag="ACHK49_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO9264484.1"
                     /translation="MQRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKTREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGD"
     gene            complement(1192..1440)
                     /locus_tag="ACHK49_003931"
     CDS             complement(1192..1440)
                     /locus_tag="ACHK49_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO9264485.1"
                     /translation="MVHDRIAEELEAKGFYRRASARWGEVMQLVETDKERHQVTMRRL
                     ECSRKAQRPPEPPTENYADLRNAVNRTYADMGMSKLAE"
     gene            complement(1451..1861)
                     /locus_tag="ACHK49_003932"
     CDS             complement(1451..1861)
                     /locus_tag="ACHK49_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO9264486.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVPLPCSQADDGT
                     ATLWLSVMAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRRGYQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDGIPF"
     gene            complement(1858..2109)
                     /locus_tag="ACHK49_003933"
     CDS             complement(1858..2109)
                     /locus_tag="ACHK49_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264487.1"
                     /translation="MTKMRRDRTAPKYKALDMTEHALKVAIRTIDRHAGEGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(2312..3721)
                     /locus_tag="ACHK49_003934"
     CDS             complement(2312..3721)
                     /locus_tag="ACHK49_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO9264488.1"
                     /translation="MKLAPNVKLLPKDKYTEAIIFAGNNAHSFAEHYIIAQAKKAGDP
                     VPPVYLGRYQLSELDNLQIVDDGRYRATVIRAGNIEEPQLLTIATKLAIAGVQEARLL
                     SENFELLEEWNDQLPRLREAWERGESLVMKKIPQRKTTLPMSVGSTGYDTQLDYVVKG
                     IIPASSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGRRVAHGAVLYVVGEGGIGVPR
                     RVKAWEVVHGEQVKNLYLVNRPIFPAVPLDVDELVIAASQVERETGKPVRMIILDTLA
                     RCFGGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDA
                     EYRIRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDKPR
                     PPVELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKRLGYDVKNMRRWL
                     AKLEKDAVIYIDGDDVGPL"
     gene            complement(3718..4017)
                     /locus_tag="ACHK49_003935"
     CDS             complement(3718..4017)
                     /locus_tag="ACHK49_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9264489.1"
                     /translation="MKLKYPGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFDRDFSLVQAVPRNVPTSREKARANIQEIRKMLNVFRGKK"
     gene            complement(4023..4250)
                     /locus_tag="ACHK49_003936"
     CDS             complement(4023..4250)
                     /locus_tag="ACHK49_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264490.1"
                     /translation="MHKIPYDVLIHSENALIKAREMNALLIKLIDVPEDGGEPASLIF
                     AAVQTLLSPVIDELDKAMAIHENNKAPHTGE"
     gene            complement(4243..4707)
                     /locus_tag="ACHK49_003937"
     CDS             complement(4243..4707)
                     /locus_tag="ACHK49_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO9264491.1"
                     /translation="MSKSRLQKIITGLYASPVAHMATGFSSLNTSGGQPPISDKRFFC
                     VRKPTHTRIMAGRNGGAFALAGFQMTSLLTLLRLATPFSSVVARLLKFHLGAFHMAVS
                     ARPYFVWRFMQCHHSSIDIYTVTAATEREARAQLPRAHLIFVARIRQGVSHA"
     gene            complement(4700..4897)
                     /locus_tag="ACHK49_003938"
     CDS             complement(4700..4897)
                     /locus_tag="ACHK49_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO9264492.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGSHHE"
     gene            5087..5392
                     /locus_tag="ACHK49_003939"
     CDS             5087..5392
                     /locus_tag="ACHK49_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO9264493.1"
                     /translation="MATANKNAKSQLTTVRVPLDVMQGMESVKLDGESNAGFIVTAMR
                     GEIARRQAEGSGENPLVSSLDALAKVEQIGIKAAEDIGQLVTVAREELQRRKTQESE"
     gene            5450..5626
                     /locus_tag="ACHK49_003940"
     CDS             5450..5626
                     /locus_tag="ACHK49_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264494.1"
                     /translation="MTNEESTEKTSPARKRRRRKKEHESERFAPCSFALEKYLKEYSS
                     KERASQLWQRTEPD"
     gene            complement(5891..6079)
                     /locus_tag="ACHK49_003941"
     CDS             complement(5891..6079)
                     /locus_tag="ACHK49_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9264495.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(6454..7683)
                     /locus_tag="ACHK49_003942"
     CDS             complement(6454..7683)
                     /locus_tag="ACHK49_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9264496.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHEMNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYDPLKKRLNNHVIQHIGPMPLDKCELRHWLACF
                     DRVAKKAPVSAGFLLQTCKQALKFCRKRRYAISNVLDDMSVADVGKKPDISERVLSNK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGCRTVELRLSEIGEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLVHRRLKHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYIDAKRNALDMWVERLEI
                     LAGTHENVTTLPVTRIK"
BASE COUNT         1763 a         2071 c         1829 g         2135 t
ORIGIN      
        1 taatcaccgt tcgcgttctt caccttctga agttttgccg ccgtcgtgga gttaaccacc
       61 cagaccgcgt ttttgcggta ttttttacgt aatgcaaatt tcaggtcgat caacgggtcc
      121 gcagatgtcc acgccagaga ttcggaaggt ttaattacct gcaacgtacc aaaatcacgc
      181 tctttgtcgt tcttctctgc acggggtacg gataaaaaac cttttgcttt tttgtcaccg
      241 tcgcccacaa ccagatcgct ttcttcggtt tccgtgaagg tgtcgccaat ctcacccgtc
      301 agccatgaaa ggatgtccac atcggaaaaa tccacgattt cctgtgtggt gcgcgggtac
      361 gcatagaccg gatacagctt aatgctcacc tcgttaatct gcggggtgct ggtctgttcg
      421 cgtgtcttac cctcttcacc gtggttaacg gtcgcaccgc cagcggaaac aagctgctta
      481 aactcgttgc tgctgatttt cttcacggta cagatgcggc gcatggtgga ctcatccgcc
      541 agcattcgca tgatttcggt gttcagctcg gggataacgg tataaccacc atcagcggga
      601 acgcctgtac ttaatgcgcg ggtttcaccc gtcagaatgt agtttcgtag ttctgcgggg
      661 tcagtggtct ggctgctttt acctggcttg ctgcgctctt cgtctgcaat ggcttcaagg
      721 cgggaaatgt ctttatcgag ggattcagct ttagcgcgta attcgtcaaa ttttgcgccc
      781 tcagcatcgt taagactgcg gttttctttt tcagcgttct ccagcatgtc gcgcatctga
      841 tttttaatgg cggttttctg ctggcgtaat tcgattattc tctgcataaa aaaaagtcct
      901 ggggttaagt aaggaactcc aggacgcggc aaacactcaa ccgtttttca taaggaaatc
      961 agtaatcgca ccgatcgttt tcccgcctgg taatgaatat tggcgggcac attaacaggc
     1021 gggaaagtgg ccccagcgtc ctggcaccac gggcgagaat aatcatgatt cagttcgggt
     1081 aaaatatgcc gatccgctca gtgaacaacg tggaacaacc acgaacaaat aatttacaaa
     1141 aaataacaaa aagccggatt gctccggctg cgtggtgtgg ttctgatatt cctactctgc
     1201 taatttgctc atccccatgt ctgcgtaagt gcgatttact gcatttctca ggtctgcgta
     1261 gttctccgtt ggcggctccg gtggcctctg tgccttcctg gaacattcca gccgtcgcat
     1321 cgtaacctga tgccgttcct tgtctgtctc caccagttgc atgacttcac cccatcgcgc
     1381 cgacgccctc cggtaaaagc cttttgcctc gagttcctcc gctatgcggt catgtaccat
     1441 cgtcaccccc tcagaacgga ataccgtcac cgtaagggtc atcgcctccc gctggtggct
     1501 gattaccctg tgtgcctgtg gttttgcgtc tgttcccgcc tggacgtgcc gcgcgggcac
     1561 tgattacgct gtctgcaata acctgatacc cccgccgcgt ttccccgttc tgtccggtcc
     1621 actggcttac ctgcatcgtg ccggatacgc tggcaacatc gcctttttga tgtttagcca
     1681 ggaaatcggc ctgtttgcca aatgccatca ccgatagcca taacgtagcc gttccgtcat
     1741 ctgcctggct gcacggaagg gggaccgcca tacgcgccag cgtcatcggt gtgcccttgc
     1801 tggtctgttt tacctgcggg tcgtccacca gccgcccgta agcggctatc tgtgctgtca
     1861 tgattccacc tctccggttt taacgttgat ggttgttacc tgttccgctt cggcaatctc
     1921 ccgttctgtc agcgtggcaa agtttgccgc cgctgtggtc atgaatgcgc ttatcaggtc
     1981 gggatgttcc ttcgcgtatc cttcccccgc gtggcggtct atcgttctga ttgccacctt
     2041 taaagcgtgc tctgtcatgt ctaacgcttt atattttggc gctgttctgt ctctgcgcat
     2101 tttggttatc tcctcactcg cactcacttt tacgcctcac tttttaacgc gtctcacttc
     2161 gtctcgcttg agattttcgt gttttatatg tgtatgtttc ataagcattt ttttactcct
     2221 cacttttgag tatgtataca ggtgagaaag tgagcaatcg tgttaatatt ttgtaaaaac
     2281 ctcaccatta ctcacttttg ctactcactt tttacagtgg tcctacatca tcaccatcaa
     2341 tgtaaatcac ggcgtctttt tccagtttgg ctaaccatcg ccgcatattt ttcacgtcat
     2401 acccaagccg tttcatgtca tcacgtaaca gcgggatcgt gcacttgtcg ccgttctgtg
     2461 tgcgtgaacg gatgcacccc catagtgcgg tatggttttc cgtcttgttg cctgcctcct
     2521 cgatgcgctc cagttcaacg ggagggcgcg gcttatccac caccaccagc gacgtgatta
     2581 actccccgtc agcgtcggta aaaagctcca ccacccgcaa atcataggca gcctctttga
     2641 gttcctccgc gtccttcatt ttggtgcatg agataaccag cgcttcgctt cctgcgtcct
     2701 ccctgcgtat ccggtattca gcatccagtg atgcacgaaa tgcactggaa ccgcgcgcgc
     2761 ctttcgtctc atccttgccg gaatggtgaa ccaccagcac cgtggcccct gtgcgccgtt
     2821 tcagttcgtc acacccacgg ataaacgccc ccatgtcacg ggaatcattt tcgtcattgc
     2881 cgccaaagca acgcgccagc gtgtccagaa taatcatgcg aacaggttta cccgtttccc
     2941 gctcaacctg gctggcagca ataaccagtt catcaacatc aagcgggaca gccggaaaga
     3001 tgggacggtt tacaaggtaa agatttttca cctgctcccc gtgcacaacc tcccaggctt
     3061 ttacacgacg cggaacgcca ataccgcctt caccaaccac atagagaaca gcgccatgcg
     3121 ccaccctgcg gcctccccac tggcggcccg tggaaacgtg acacgcccac gatccggcaa
     3181 ggaatgattt ataggaaccg ctcgccccgt atatgctgca aagcgacgat gccggaataa
     3241 tcccctttac cacgtaatca agctgtgtgt cgtatccggt agatccaacg ctcatcggta
     3301 gcgtggtttt tcgctggggg atttttttca tgaccaggct ttccccgcgt tcccatgcct
     3361 ccctaagccg tggaagctgg tcgttccatt cctccagcaa ttcgaaattt tcagaaagta
     3421 gccgcgcctc ctggactccg gcgatcgcca gtttcgtggc gatggttaac agctgcggct
     3481 cttcaatatt tccggcgcgt atcaccgtcg ccctgtatcg cccatcatca acaatctgga
     3541 ggttatccag ttcgcttaac tgataacgcc ccagataaac gggagggact ggatcgcctg
     3601 cttttttggc ctgtgcaatg atgtagtgct ctgcaaatga gtgcgcatta ttccccgcga
     3661 aaataattgc ttcggtgtat ttgtctttcg gtaatagttt tacgttcggt gcaagtttca
     3721 ttttttaccc ctgaatacgt tcaacatctt tcttatttcc tgaatattgg cgcgtgcttt
     3781 ctccctgctg gtgggcacgt tacgcggtac agcctgcacc agagaaaaat cacggtcgaa
     3841 ctgataaaca ggcataacgc aatcatattc gtagccttca cggcggtaag ttacgcgccg
     3901 ttcctccacg cccttaatca ttaccgtgcc gccgtactgg tcgcggtaaa tatcaccgcg
     3961 cgtaaattta gggtgagtgt tgccactggc agttaagcca ggatatttaa gtttcattat
     4021 ttttattctc cggtgtgggg cgctttatta ttttcgtgaa ttgccatcgc cttatccagt
     4081 tcatcaatta caggcgatag taatgtctga acggcggcaa atattaatga tgcaggttct
     4141 ccaccatctt ccggcacgtc tattaactta attaacagcg cattcatttc acgcgcttta
     4201 attaatgcgt tttcagaatg aataagaaca tcatagggta ttttatgcat gactcacccc
     4261 ctgacgaata cgggcgacaa aaatcagatg tgcgcgcggc aactgggcgc gggcttcgcg
     4321 ttcagtggct gcggtaacgg tgtaaatatc aatgctgctg tggtggcact gcataaagcg
     4381 ccagacaaag taagggcgtg cggatacagc catgtgaaag gctcccagat gaaatttaag
     4441 gagtctcgct actacgctgc taaacggggt ggcgagacgt aacagggtta gcagactggt
     4501 catctggaaa ccagcgagcg caaaggctcc cccgttacgc cccgccataa tgcgggtatg
     4561 ggtaggttta cggacacaaa aaaaccgctt atcggatatg ggcggctgtc cgccagatgt
     4621 attcaggctg ctaaacccgg tcgccatgtg ggcgacgggt gaagcataca gccccgttat
     4681 aattttttgc aagcggcttt tactcatgat gtgacccctg acgaatacga gcggcaaata
     4741 cagcaacaca acctgacggg caacggctac gcgcttcgcg ttccgtccag gcggttacgt
     4801 ggatgatttg agattctccg gcactcaggg ccagaaaacg ccacacaaag gccgtttgtg
     4861 tgtgtacaag gtgtggtata tgatttacgg caaccataac ggctcctagt ttacgttgtt
     4921 ggttagaagc cccgttactg ctcctacagt gcggggcttt gctctttgta tttcaacaat
     4981 ccttttggtg tgtttcatgt tatggacaca tgaaacacac gtcaaggctt tttgtatttc
     5041 tttttttgtg tatactgaaa cacaccgatg attaggagtt tcagaaatgg caacggctaa
     5101 caaaaacgca aaatcacaac tgacaaccgt cagagtccca ctagatgtta tgcaagggat
     5161 ggaatccgtt aagctggacg gcgaaagcaa tgccggattt atcgtaaccg ccatgcgcgg
     5221 tgaaatcgcc cgccgccagg cagaaggaag cggagaaaat ccccttgtgt cttcactgga
     5281 tgctttggct aaggtcgaac aaattggcat caaggcagcg gaggatatcg ggcagcttgt
     5341 caccgttgca cgcgaagaac tccagcgtcg caagacccaa gaatcagaat aatcactatc
     5401 agcgccgtgg cgtgaggtac tacggcgcat tgctttacag gcgcacacaa tgaccaacga
     5461 agaatcaacc gaaaaaacat cacctgcacg aaaaagacga cgcagaaaga aagagcatga
     5521 atcagaacga ttcgcgccgt gttcttttgc ccttgagaag taccttaaag aatactcatc
     5581 aaaggagaga gcttcgcagt tatggcaacg cactgaacca gactgattgc attgcacacc
     5641 agcctgatag cggctatcat ccccgtgctt atgtttggga tcacacacac aaactgcgca
     5701 gcaggttaat tgttcagaaa ggcggctcca tatcggggcc gcttttttta tgcctgaaaa
     5761 accccaattt tgttgttttt cagtttcacc agggcgaacg aatccccgcc cacgttcggg
     5821 cgcatattca atcttcatag ttatatctct gtattagtgg atgtgtggcg actgtgtgcc
     5881 gccagtcttt ttagtgaact gccttgcagc tatccttcca ggccaaaacc tcagataaag
     5941 accagccaac agaacgccca ccaagtttac gacgtgatgg gaattgtccg gccttttcca
     6001 ggcggtagcg gcatgagcgg ctaaggcctg ttagcttttc gcattctttt tcacgtataa
     6061 accgatcagt gcttaacact attgccccct ttcgtttctt aaagagttat ttcgtgttct
     6121 attgcgttgg gatgtgtctg attgtgtcag gatgattcag agttggcaaa tgttggtgtc
     6181 gcatggttta cagaaagagg aataatcagg ataaaatcat ttaaattcat atcaatacaa
     6241 aggcataaaa tcttgtttta tgcctttttt cgcacgcttt aacgcgtaat tcactaatgt
     6301 ataaaaaacc agctaagcat taaaaatcag taacttataa atctgtattc tttttggcct
     6361 cttgttcgtg attgtgtcac gttgttgcac attgtttcac gttgtatctg tgcacttatc
     6421 cagtatgcgc atactgaaaa aacacgaaaa aaattatttt attctggtta ctggtagcgt
     6481 ggttacgttt tcatgtgttc ccgccagtat ctccaaccgc tccacccaca tatcaagcgc
     6541 attgcgtttc gcatcaatat agcgggaatg gttgtacacg cgttgcattc ctggcatctg
     6601 gtggcctgta agctgctcca cgacgtgagg atcaacgcct aaatcgttca gcatggttgt
     6661 aaaggtgcgc cggatgtcat gcagtgacca gtgagggtgt ttaagcctcc tgtgcaccaa
     6721 tctgccgtac tgcgatacgc tggtttcctg tttcacttcc cccagcaata agcccgtgtg
     6781 cctgttctgc tccaccagct gcgtgacgaa cggcagtatc gcttccggta tgggccggaa
     6841 tatggctacc ttcgttttgc tgtgctcctt cggaacggtc cagagcattt cggtaaaatc
     6901 ccactcgccg atctccgata acctcagttc tactgtccgg catccgaaaa caatcaggag
     6961 gcggattagt gcgacgtagt aaggggaaaa tatttttttg tccagtgcct gcaataattc
     7021 gccaagttct ttgttactta agacacgctc acttatatcc ggttttttcc caacgtccgc
     7081 cacactcata tcatcaagaa cgttgctgat tgcatagcgc cgcttcctgc agaacttaag
     7141 cgcctgcttg catgtctgta gcaggaaccc ggcggaaaca ggggctttct ttgccacccg
     7201 atcaaaacag gccagccagt gccgtagctc gcatttatcc agcggcatag ggccaatgtg
     7261 ctgtattacg tgattattta gccgcttttt cagtgggtca taatccacac ggttttcctt
     7321 tgcgtacgac tcaagccagt aggtgagcgc atcgccaacc gttaccggct ttaacgcttc
     7381 ctgtacggtg taattcattt catgacgtgg atttttcccc tctgccagcc atgcgcgaca
     7441 ctgggcggct ttttccctgg ctgatttcag gctcagatca ggataatttc ccagcttaat
     7501 gcgttccggt ggtgcccccc ttcccgttcc ggccctgtaa gtgaaatacc aggttaaaag
     7561 gccactggtt gaatgcctga cgctcaggtt tccaccgtca ttaagaaagg ctgttttttg
     7621 ggcgggtgtg ccgttgattt tcctcagctg tgtatcgctc agtttgttaa gtgctctgct
     7681 cataattttg aatccggtca tcacaattaa ctacaccatt aactacaccg atcgttgcac
     7741 aaagggctac aacgtgaaac aagctggaac aagaaaaatc acaaatctgt ttataatc
//
LOCUS       DAZGLJ010000201         7769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000201 DAZGLJ010000000
VERSION     DAZGLJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.201"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1372
                     /gene="xanP"
                     /locus_tag="ACHK49_003943"
     CDS             <1..1372
                     /gene="xanP"
                     /locus_tag="ACHK49_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO9264497.1"
                     /translation="SENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLLAMFVAVI
                     TPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQGTSFNFVA
                     PLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPLVSGVVVM
                     IIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNPYLRVASL
                     VIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLMLVFMITSL
                     ETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCFGQNNGVI
                     QLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIAASGVRIV
                     SREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGITAIVLNLI
                     FPPEKQ"
     gene            1493..3202
                     /gene="yicH"
                     /locus_tag="ACHK49_003944"
     CDS             1493..3202
                     /gene="yicH"
                     /locus_tag="ACHK49_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO9264498.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRINGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDVAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(3255..5573)
                     /gene="yicI"
                     /locus_tag="ACHK49_003945"
     CDS             complement(3255..5573)
                     /gene="yicI"
                     /locus_tag="ACHK49_003945"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO9264499.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGNNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(5583..6965)
                     /gene="yicJ"
                     /locus_tag="ACHK49_003946"
     CDS             complement(5583..6965)
                     /gene="yicJ"
                     /locus_tag="ACHK49_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO9264500.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            7258..7352
                     /locus_tag="ACHK49_003947"
     tRNA            7258..7352
                     /locus_tag="ACHK49_003947"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7292..7294,aa:Sec,seq:tca)
     gene            7431..7559
                     /gene="rhuM"
                     /locus_tag="ACHK49_003948"
                     /pseudo
     CDS             7431..7559
                     /gene="rhuM"
                     /locus_tag="ACHK49_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
BASE COUNT         1871 a         2061 c         1955 g         1882 t
ORIGIN      
        1 gtcagaaaat gcgcaaccgg ttgcgcagac tcaaaacagc gaactgattt accgtcttga
       61 agatcgtccg ccgcttcctc aaaccctgtt tgccgcctgt cagcatctgc tggcgatgtt
      121 cgttgcggtg atcacgccag cgctattaat ctgccaggcg ctgggtttac cggcacaaga
      181 cacgcaacac attattagta tgtcgctgtt tgcctccggt gtggcatcga ttattcaaat
      241 taaggcctgg ggtccggttg gctccgggct gttgtctatt cagggcacca gcttcaactt
      301 tgttgccccg ctgattatgg gcggtaccgc gctgaaaacc ggtggtgctg atgttcctac
      361 catgatggcg gctttgttcg gcacgttgat gctggcaagt tgcaccgaga tggtgatctc
      421 ccgcgttctg catctggcgc gccgcattat tacgccgctg gtttctggcg ttgtggtgat
      481 gattatcggc ctgtcgctaa ttcaggttgg gttaacgtcc attggcggcg gttacgcagc
      541 catgagcgat aacaccttcg gcgcaccgaa aaatctgctg ctggcaggcg tggtcttagc
      601 cttaattatc ctgcttaacc gtcaacgtaa cccttactta cgcgtggcct cactggtgat
      661 tgcgatggcg gccggatatg cgctggcgtg gtttatgggc atgttgccag aaagcaacga
      721 accgatgacg caagaactga ttatggtgcc aacgccgctc tattacggtc ttggcattga
      781 atggagtctg ctgctgccgc tgatgctggt ctttatgatc acttcgctgg aaaccattgg
      841 cgatatcacg gcgacctctg acgtttccga acagccagtg tccggtccgc tgtacatgaa
      901 acgcctgaaa ggcggcgtgc tggcaaacgg cctgaactcg tttgtttcgg cggtgtttaa
      961 caccttcccg aactcctgct tcgggcaaaa caacggagtg atccagttga ctggtgttgc
     1021 cagccgctat gtcggttttg tcgtcgcgct gatgttgatc gtgctgggtc tgttcccggc
     1081 agtgagcggt tttgtacaac acattccaga accggttctg ggcggcgcaa cgcttgtaat
     1141 gtttggcact atcgccgcct ccggtgtgcg tatcgtttct cgtgaaccgc tgaaccgtcg
     1201 ggcgattctg attatcgcgc tgtcgctggc ggttggtctg ggcgtgtctc agcagccgct
     1261 gattttgcag tttgcccctg aatggctgaa aaacctgctc tcctccggga tcgccgcggg
     1321 cggtattact gccatcgtgc tgaatctgat tttcccacca gaaaaacagt aattcactgg
     1381 cggcggtaag ataattaccg ccgcagtctc acctctttca ccattcccgc cttgaggatt
     1441 gcgcgtaaat cgtgcataac tcccttatgt gcatttcacg ggatggaaga ccatgaaatt
     1501 tattgggaag ctgcttctct acattctcat cgctctgtta gtggtgatcg ctggcctcta
     1561 ttttcttctg caaacccgct ggggagcaga acatatcagc gcatgggttt ccgagaatag
     1621 cgactatcat ctggccttcg gggcgatgga tcaccgtttt tccgcgccat ctcatatcgt
     1681 gctggagaac gtcacgtttg gccgtgatgg ccagcccgcg accctggtgg caaaaagtgt
     1741 cgacattgcg ctaagcagtc ggcaactgac cgaaccacgc catgtcgata ccatcctgct
     1801 ggaaaacggg acgctgaatc tcaccgacca gaccgcgccg ctaccgttca aagccgatcg
     1861 tctgcaactg cgtgatatgg cgtttaatag cccgaatagc gaatggaaac tgagcgcgca
     1921 gcggataaat ggcggcgtag ttccgtggtc accaaaagcc ggtaaagtgc tgggtacgaa
     1981 ggcgcagatt cagtttagtg ccggatcgct ttcgctcaat gatgttcctg ccaccaatgt
     2041 actgattgaa ggcagtattg ataacgatcg cgttacgctg actaacctgg gtgccgacat
     2101 cgcccgcggg acattaaccg gaaacgcgca gcgtaacgcc gacggcagct ggcaagtgga
     2161 aaacctgcgc atggcggata tacgtctaca aagcgaaaaa tcgctaaccg acttctttgc
     2221 gccattacgc tctgtcccgt cgttgcagat tggtcgcctg gaagtgatcg atgctcgttt
     2281 gcaaggtccg gactgggcgg tgaccgacct cgatctcagc ttgcgcaaca tgaccttcag
     2341 taaagatgac tggcagacac aggaaggcaa actgtcgatg aacgctagcg agttcattta
     2401 tggttcgctg catttatttg acccgattat aaacgcggaa ttttccccgc agggcgtagc
     2461 gctgcgccag ttcaccagcc gctgggaagg gggtatggtc agaacgtcag ggaactggct
     2521 gcgtgacggg aaaacgttga tccttgatga tgtggcaatt gccgggctgg aatatacctt
     2581 gccgaaaaac tggcaacagt tgtggatgga aacgacaccc ggttggttaa acagcctgca
     2641 actgaagaga tttagcgcca gccgcaatct gatcattgat atcgaccctg acttcccgtg
     2701 gcagctcacc gcgctcgatg gttacggtgc caacctgacg ctggttaccg atcataaatg
     2761 gggcgtctgg agtggctcgg cgaatctgaa tgccgccgcc gcgacattca atcgtgttga
     2821 tgttcgtcgc ccgtcgctgg cgctgaccgc caacagcagc acggtgaata tcagcgaact
     2881 gagtgcattt actgaaaaag gcattctgga agccactgcc agtgtttcac aaacgccaca
     2941 acgtcagacc catatcagcc tgaatggacg cggtgtgccg gtgaatattt tgcaacaatg
     3001 gggatggcct gaattaccgt tgactggcga cggcaatatt cagcttaccg ccagtggaga
     3061 tattcaggcc aatgccccgc tgaaacctac ggttagcggg caattgcatg ccgtgaacgc
     3121 cgcaaagcag caagtgactc aaaccatgaa tgctggcatc gtttccagcg gtgaagttac
     3181 gtcggcagag ctggtgcagt aaacatcaat ctcgccgggt acgactcaga cgtacccggc
     3241 attccatcaa tagattacaa cgtaattgtc agcgcattcc cttgaggctt caccaccagc
     3301 ccctgctcac tttcagcctg cgaaccgtct tgcagaccat ttactttcac aacattgcgc
     3361 aggcacagtg tccagttctt cgcctcgccc gcaccagtca cagtaatcgt gttgccagta
     3421 cgtgctgctt ttaaagtaaa gatcaccgat ccgtcagcag cgggcacttc acagacggct
     3481 tcatgcccgt cttgcagatt aaagaggtgg aatgccgtgc cttcgtgcca cacgtaatcg
     3541 ggacgttgat cgttgttgcc cagcgccagt agagtgttat cacgcacata aacgggcaga
     3601 ctcaggaagc cgtgctgctg tttatgccag cgactaccgt cgagttcatc gttgtgccac
     3661 aggtgtgtcc agcgaccttc cggcaggtag aactgcacat cgcccgcttc agtgaacacc
     3721 ggcgcaacca tcacgttgtt gcctaacatg tattgacggt caaggtaatc acaagccgga
     3781 tcgtccggga actccatcat catggcccgc atcatcggcg taccccgcgc gttcgcacgc
     3841 gcagcttcac gatacagata cggcatcatg cggcatttca gttgcgtgaa gaagcgcact
     3901 acatcacagg actcatcatc gtaggcccac ggcacacgat aagatttgct accgtgtaaa
     3961 cggctatggc tggagagcaa accaaacgcg caccagcgtt tgtaaacgtg cgccggagcg
     4021 gtattttcaa agccgccgat atcgtggctc cagaagccaa aacctgaaag gccaatagac
     4081 aaaccaccgc gcaggctttc cgccattgat tcgtagttag cgtaacaatc gccaccccag
     4141 tgtaccggga atttctgcgc accgacggag gccgagcggg caaacaagac agcttcttcc
     4201 tcaccaacgg tgtccttgag cacgttccac accagttcgt tgtagatgta tgcataatgg
     4261 ttatgcattt tctgcggatc ggaaccgtca aaccactgaa catcagttgg gatacgttcg
     4321 ccaaagtcgg tcttaaagca atcaacgccc atcgcgacca gacctttcag tttgtcggcg
     4381 taccatttgc aggcatccgg attggtaaag tcataaatcg ccagacctgg ctgccattta
     4441 tcccactgcc atagcgaacc gtccgggcgt ttgagtaaat agcctttctc ttgtaactct
     4501 ttaaagacgg gggatttttg accgatatag gggttaatcc agacgcagat tttcagccct
     4561 ttcgctttca ggcggcggat catcccttcc gggtcaggga aagtcagcgg gtcccactca
     4621 aaatcgcacc actggaaggc tttcatccag aaacagtcaa agtggaaaac atgcagcggc
     4681 agattgcgtt ccgccatacc atcgataaag ctgtttaccg tcgcttcgtc gtagttggtg
     4741 gtaaatgaag tggttagcca caggccgaag gaccacgcgg gcggcagcgc cggacgacca
     4801 gtaaagcggg tataacgatc aagtaccgct ttcggcgtcg ggccgtcgat aacaaagtat
     4861 tcgagatatt cactctcaac gctgaactgc actttggaga ctttctccga tcccacttca
     4921 aaagagacac actggggatg attgaccagt accccataac cacggttagt catgtagaac
     4981 gggatatttt tatacgcctg ttcagtactt gtgccgccgt cccggttcca ggtctctacc
     5041 gtctggccat tgcgcaccag ggcagtaaag cgctctccca gaccgtaaac tgtttcgcca
     5101 acgccaagat caagccgctc aaacatataa ttgcgttgat tattcgtgtc ctgcacgtag
     5161 ccattatttt tcacctgact accggtaata cgttcgccgt tgcgcagaaa atccagtgac
     5221 cagaactcac ctttgctgac acgcgcgctt aagttgccac ttttaaactc agcataacgt
     5281 tctgtgtttt cgattgtgac cttcacgtcc tgcaaaatat tgagcggata atgaggaccg
     5341 ttattcagcg ccccctgaaa atgctcaatc cgcacaccga caataccttc ctgtggggag
     5401 aaaaagcgca gcgtaaataa aggcgtatca agctgccagg tacgttcacg cacatcacgg
     5461 ggggcagcat agaccaccat ttcattaccc tgctgttcaa cctcgaatac ctgaagcggg
     5521 tgaatcaaat tgaggccagg ttgaatcaac cagtttccat cgctaatttt cattgccgtt
     5581 ccttagttct gcacttcttg agaggtgaat tgttgctggc aacggcgttt accctgagcc
     5641 agctgttcca taacggtttt cagattgtgc gtcgtgagtg agtagtagcg tttagcgata
     5701 atcgcgctca gcaaataaca gatcgccgga acaatcgtga atagcgcaat aatgatgcta
     5761 atcgtggcgc tgttctgcgc tttttctgcc gcatcatatc cgccataagc cagcatccag
     5821 ccgataagag cgccgccgaa ggccaacccc agtttgagca caaacagcgt gccagcaaaa
     5881 ctgatcccgg tcaggcgttt accattgcac cactcgccgt agtcgacggt atcggacatc
     5941 attacccact ggataggtgt caccagttga tgcaacacac caatcacgaa gatgaagaca
     6001 aacatagtga tgctggcctg catgggaaca aagaacatcg cgaggctaat cactgccagc
     6061 agggcgttcg tccaccagaa gatagtgact ttacatttcc agtcggtcag aggttttgcc
     6121 agtgcggaac caatcaggtt acccacgcaa taagtggtga gaaaagcgac aaacacttcc
     6181 ggcgtgccca aaatccatgt gacgtaatac atcatcgccc caccgcgtac gcacaccgcc
     6241 aggatattga aaatggttag taaaccgaca atccgccact ggtcgttttg ccagatatca
     6301 cgtaaatctt cccgcataga cgttgttgta ggtggtgctt caacgcgttc tttagtggtg
     6361 aagaaacaga atgccagcat catgaatgcc accacggaaa ggaccgcgat accgccctgg
     6421 aaaccgagtg gtttattatc accgccaatt aaattaacca gtggcatcat cagaacagta
     6481 gaaagcatgc ctcccgcggt cgccagcaca aaacgccagg attgcagcga gatacgctga
     6541 gtcgggtcat tggtgattac accacccaat gcgcagtaag ggatattgac gacggtataa
     6601 agtaaggtaa gtagggtgta agtaattgct gcatagatca ttttgccgtt catactgaga
     6661 tctggcgtgc tataggccag tacacagacg atcccgaatg gcagtgcgcc aaacagtacc
     6721 cacggacgaa atttacccca gcgagagcgc gttcgatcgg ccaacaaccc catgcaagga
     6781 tcggaaatcg catccagtgc acgagcgacc aaaaacatgg ttccgacaaa accggcagga
     6841 atgccaaaaa tatcggtata aaagaacatc atatataaca ttacgttatc gaaaataatg
     6901 tggctggcgg cgtctcccat gccataacca attttctctt taacggacaa cacttcactc
     6961 ttcatctgct tttccttgat gctatagggg tgctgagacc gataacgcat tttgtaaacc
     7021 atccctgtga gagcgaatat tccgatatct ggttattaat ttatgattct tgttttatgt
     7081 gatcgtggta gcgttaattt cgctcatata tcattgtaaa atataggttt tatatgaact
     7141 ataatgcttt cgtgataata cgctgcgtgt attaggcgga aaaaactgat ctgggggatg
     7201 tagaaactca aggaagtagc tataatgcgc cccgcctcca tgtagcaatc gaggcgcgga
     7261 agatcgtcgt ctccggtgag gcggctggac ttcaaatcca gttggggccg ccagcggtcc
     7321 cgggcaggtt cgactcctgt gatcttccgc caaaatgcct cttctgacgt tctccaaccc
     7381 cttgtaatac tgtcaatcca gatattttct actgctcaat attcaatcgg cttcaggaat
     7441 acctggtcta aggttttgtg atggacgatg agcggctgaa gaatccgcct gtcggttctt
     7501 ctgtcctcga acgcatccgc gatatgcgcg ccagcatgtg ccgtgtttat ttgcaggtga
     7561 tttgccccct gctggctctc agccaactgt atcgccatta aggtgaagat gttgttttac
     7621 tcgccccaga acaccctttt ttatacgact attctatttc tttaaccgct ggcgaaaaaa
     7681 actgactata aacttcacca ataatcagac tgccatcaag attaatttgt tcatttaata
     7741 tatcaaatca acttgtttta tttttacta
//
LOCUS       DAZGLJ010000202         7763 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000202 DAZGLJ010000000
VERSION     DAZGLJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.202"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            142..684
                     /gene="ecpR"
                     /locus_tag="ACHK49_003949"
                     /gene_synonym="matA"
     CDS             142..684
                     /gene="ecpR"
                     /locus_tag="ACHK49_003949"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO9264501.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            758..1345
                     /gene="ecpA"
                     /locus_tag="ACHK49_003950"
                     /gene_synonym="matB"
     CDS             758..1345
                     /gene="ecpA"
                     /locus_tag="ACHK49_003950"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO9264502.1"
                     /translation="MKKKVLAIALVTVFTGIGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1403..2071
                     /gene="ecpB"
                     /locus_tag="ACHK49_003951"
                     /gene_synonym="yagY"
     CDS             1403..2071
                     /gene="ecpB"
                     /locus_tag="ACHK49_003951"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO9264503.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2097..4622
                     /gene="ecpC"
                     /locus_tag="ACHK49_003952"
                     /gene_synonym="yagX"
     CDS             2097..4622
                     /gene="ecpC"
                     /locus_tag="ACHK49_003952"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO9264504.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAEVTQTGEENES"
     gene            4612..6255
                     /gene="ecpD"
                     /locus_tag="ACHK49_003953"
     CDS             4612..6255
                     /gene="ecpD"
                     /locus_tag="ACHK49_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO9264505.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6224..6934
                     /gene="ecpE"
                     /locus_tag="ACHK49_003954"
     CDS             6224..6934
                     /gene="ecpE"
                     /locus_tag="ACHK49_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO9264506.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7247..7576
                     /gene="ykgJ"
                     /locus_tag="ACHK49_003955"
     CDS             7247..7576
                     /gene="ykgJ"
                     /locus_tag="ACHK49_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO9264507.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7571..>7763)
                     /locus_tag="ACHK49_003956"
     CDS             complement(7571..>7763)
                     /locus_tag="ACHK49_003956"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264508.1"
                     /translation="FYILIQIVSEYIALLQDDVDPKNATEERHCCWQIEEVSGFVTPE
                     KRSPAIIAGGAVQLIIVCL"
BASE COUNT         2015 a         1987 c         2006 g         1755 t
ORIGIN      
        1 ttaaccttta ttgatatgta ctacgtatct tatttacttc cggtttacta acgaaactga
       61 atgcacctgt aaaaattaca ggtttggaaa gtagtgacat ggcaaagtga ttacagcagg
      121 gactatgagg ttaaaaacca tatggaatgt caaaaccgtt ctgataaata catctggtct
      181 ccccatgacg cctacttcta taaaggacta tctgaactga ttgtggatat cgacagatta
      241 atttatctat cgttggagaa aattagaaaa gatttcgtgt ttatcaatct caatacggat
      301 tctttatctg aatttataaa ccgtgataat gaatggttat ccgcggtaaa ggggaaacag
      361 gtcgtattga ttgcggccag aaagtcagaa gccttagcaa attattggta ttacaacagc
      421 aatattaggg gcgtggtata cgcaggactg agtcgtgata ttagaaaaga actggcctat
      481 gtgattaatg gcaggttcct gagaaaagat attaagaaag ataaaatcac ggaccgggaa
      541 atggaaatta tccgcatgac ggcccaggga atgcaaccta aatcgattgc cagaattgaa
      601 aattgtagtg tgaagacagt gtatacccat cggcgtaatg ctgaggccaa gctgtactca
      661 aaaatatata agttggttca gtaaactcca ggctagttag ttttaaaaat gactcactgg
      721 gacatcacgt cctcaattca actcgggaag aaatgcaatg aaaaaaaagg ttctggcaat
      781 agctctggta acggtgttta ccggtatagg tgtggcgcag gctgctgacg taacagctca
      841 ggctgtagcg acctggtcag caacagccaa aaaagacacc accagtaagc tggttgtgac
      901 gccactcggt agcctagcgt tccagtatgc cgaaggcatt aaaggtttta actcacagaa
      961 aggtctattt gacgtggcta tcgagggtga ctcaacggct accgccttta aactgacctc
     1021 acgtcttatc accaacacat taacccagtt ggatacctca ggttccacac tgaatgtggg
     1081 cgtggattat aacggcgcgg cagtcgaaaa aactggcgat accgtgatga tcgataccgc
     1141 caacggcgta ctgggcggca accttagccc gctggcaaac ggttacaatg ccagcaatcg
     1201 taccaccgca caggatggtt tcactttctc catcatcagc ggcaccacca atggtaccac
     1261 cgcagtaacc gattacagca ctctaccgga aggcatctgg agcggcgacg ttagcgtaca
     1321 gttcgacgcg acctggacca gttaatctct ctgatgtacc agcaggggta gcccccctgc
     1381 tttattcctg gacggaattg ttatgaaaaa gcaccttctg cctctcgctc tgctgttttc
     1441 cggaatatct ccggcccagg cgctggatgt cggcgatata tcatcgttta tgaacagtga
     1501 cagcagcacg ctgagcaaaa cgatcaaaaa cagtaccgac agtggtcgcc ttatcaatat
     1561 ccgtctcgaa cggctctctt caccgcttga cgacgggcag gttatctcaa tggacaagcc
     1621 ggatgagttg ctactcactc ccgccagctt gctgctaccc gcccaagcca gcgaagtgat
     1681 ccgcttcttc tataagggac ccgcagatga aaaagagcgc tactaccgca ttgtctggtt
     1741 tgatcaggcc ctcagtgatg cgcagcgcga taatgccaac cgcagcgctg tggccactgc
     1801 ttccgcccgc atcggcacca ttctggtcgt cgcccctcgt caggcgaact accactttca
     1861 gtacgccaac ggctccctga caaatacagg aaatgcgacg ctgcggatcc tcgcttacgg
     1921 cccctgcctg aaagccgcca atggtaagga gtgtaaagag aattactacc tgatgccagg
     1981 caagtcgcgt cgttttaccc gcgtggacac ggcggataac aaaggacggg ttgcactttg
     2041 gcagggtgat aagttcattc ccgtgaaata gatagctgtg cagatggata acgacaatgc
     2101 ctttacgacg gttctcccca ggactgaaag cccagtttgc cttcggcatg gtctttttgt
     2161 tcgttcaacc cgatgccagc gctgctgaca taagtgcgca gcaaataggt ggggtgatta
     2221 ttccgcaggc cttcagtcag gcgcttcagg acggcatgag cgtcccgctc tatattcatc
     2281 tcgccggtag ccagggtcgc caggacgatc agcgaatcgg cagcgctttt atctggctgg
     2341 atgatggaca gctacgcatc cggaaaatac agctggaaga gagtgaagat aacgccagtg
     2401 tcagcgaaca aactcgacag cagctgatgg ctctggcgaa cgccccgttc aatgaggccc
     2461 ttaccatccc cctgactgac aacgcgcagc tggatctcag cttgcgccaa ctgctgctgc
     2521 agctggtggt caagcgcgaa gcgctgggca ccgtactacg ctcacgtagc gaagacatcg
     2581 ggcagtccag tgttaacacc ctcagcagta atctgagcta taacttgggc gtctataaca
     2641 accagttgcg taacggcggg agcaacacat ccagctatct gtcgctgaat aacgttactg
     2701 cgctgcgcga acatcatgtg gtgctcgacg gctcgctgta cgggatcggt agcggtcaac
     2761 aggacagtga attatataaa gcgatgtatg aacgcgattt tgccggtcac cgatttgccg
     2821 gtggaatgct cgacacctgg aacttgcagt ccttagggcc gatgaccgcc atttcagcag
     2881 ggaagattta cggcctttcc tggggaaacc aggccagctc caccatcttc gacagcagcc
     2941 agtcagccac gccagtgatc gcctttttac cggcggcggg cgaagtacat ctcacccgtg
     3001 atgggcggct actaagcgtt cagaacttca ctatgggcaa tcatgaagtg gatacccggg
     3061 gtctaccgta cgggatttac gatgtggaag ttgaggtgat cgttaacggt cgcgtgatca
     3121 gcaaacgccc ccagcgggtc aataagctgt ttagccgggg gcgcggcgtc ggtgcaccac
     3181 tggcgtggca ggtatggggc ggtagctttc atatggatcg ctggtcggaa aacgggaaaa
     3241 agacgcgacc agctaaagag agttggctag caggtgcctc gacctccggc tcattgagta
     3301 cgcttagctg ggcggcaacg ggatatggat acgataatca ggcggtgggt gaaacccgtc
     3361 tgacgctgcc gcttgggggg gcgatcaacg ttaacctgca aaatatgctg gccagtgaca
     3421 gctcatggag cagcatcggc agcatcagcg ccactctacc gggaggcttt agttcgctgt
     3481 gggttaacca ggaaaaaacc cgcattggca atcaattgcg acgtagcgat gccgacaacc
     3541 gtgcaatcgg cggcacactc aacctgaact cactgtggtc gaagctgggc acattcagca
     3601 tcagctacaa tgatgaccgc cgttacaaca gccattatta cacggcagat tactatcaaa
     3661 atgtctacag cggtaccttt ggttcgcttg gcctgcgggc cggtattcag cgctataaca
     3721 acggcgacag caacgccaat acagggaaat atatcgctct cgatctctcg ctaccactgg
     3781 gcaactggtt tagcgcaggg atgactcatc aaaacggcta caccatggca aacctgtcag
     3841 cacgcaaaca gtttgatgaa ggaaccattc gcactgttgg tgccaatctg tcacgtgcca
     3901 tctccggcga taccggtgat gacaaaaccc tcagcggtgg ggcgtatgca cagttcgacg
     3961 ctcgttacgc cagcggaacg ctgaacgtca atagcgcggc ggacggctac atcaatacca
     4021 acttgaccgc caatggcagc gtcggctggc agggtaaaaa catcgctgcc agcgggcgga
     4081 ctgatggcaa cgctggggtg atattcaaca ccgggctgga ggacgacggt cagatcagcg
     4141 ccaaaatcaa cgggcggatt ttcccgctta acggcaagcg taactatctc ccgctctctc
     4201 cctatggaag atatgaggtg gagttacaga acagcaaaaa ctcactcgac agctacgata
     4261 tcgtcagcgg ccgcaaaagc catctgactc tctatccagg caatgtcgct gtcattgagc
     4321 cagaggtgaa gcagatggtt accgtctccg gtcgtatccg tgcggaagac ggcacactgc
     4381 tggctaacgc acggattaac aaccatatcg gccgaacccg aaccgatgaa aacggcgagt
     4441 ttgtcatgga cgtggataag aaatatccca ctatcgattt tcgctacagt ggcaataaaa
     4501 cctgcgaagt ggcactggaa ctcaaccagg cgcgcggtgc cgtctgggtc ggtgatgtgg
     4561 tctgcagcgg cctctcatcg tgggcggagg tgacgcagac aggagaagag aatgagagtt
     4621 aacctactaa taacgatgat aattttcgcg ctaatctggc cagtaactga gctcagagcg
     4681 gcagtgagca aaacaacctg ggcggatgca ccggcacgcg agtttgtgtt tgtcgaaaac
     4741 aactcagacg acaacttttt cgtcactcct ggcggggcgc tggatccgcg cctgaccggt
     4801 gccaaccgct ggaccggttt aaaatacact ggttcaggaa ccatctatca gcaaagcctc
     4861 ggctacattg ataacggtta caacaccggc ctttatacca actggaagtt tgatatgtgg
     4921 ctggaaaatt caccagtttc atctccttta actggcttgc gctgcatcaa ctggtacgct
     4981 gggtgtaata tgaccaccag tcttatcctg ccgcaaacca ccgacgccag tggattttat
     5041 ggcgcgacgg tgaccagcgg cggcgcgaag tggatgcacg gcatgttgtc agacgcgttt
     5101 taccagtatc tgcaacaaat gcccgtcggc agcagcttta caatgaccat caatgcctgc
     5161 cagacctctg tgaactatga cgccagcagc ggcgcacgct gtaaggatca ggcctccggc
     5221 aactggtatg ttcgcaacgt cacccatacg aaagcagcaa atctgcggtt aataaatacc
     5281 cactcgctgg cggaggtatt tatcaacagc gacggagtac cgactctggg cgaagggaac
     5341 gccgactgcc ggacacagac catcggcagc ctttcaggat taagttgtaa gatggtcaac
     5401 tataccctgc aaacaaacgg actcagcaac acctcaatcc atatattccc ggcgatcgcc
     5461 aactcgtcgt tagcctcggc cgtcggggcg tacgatatgc agttcagtct gaatggcagt
     5521 tcatggaaac cggtgagcaa taccgcctat tactacacct tcaacgagat gaagagcgca
     5581 gactcgatct atgttttctt ctcgagcaac ttctttaagc agatggtgaa cctcgggatc
     5641 agcgatatca acaccaaaga tctattcaac tttcgctttc agaacaccac atcaccggag
     5701 tctggctggt atgaattttc tacctccaac acgctgatta tcaaaccccg tgatttcagc
     5761 atcagtatta tctccgatga atatactcag acaccgtcgc gggagggata tgttggcagc
     5821 ggcgagtcgg cactcgattt cggctatatc gtaaccacca gcggtaaaac agctgccgac
     5881 gaagtgctga tcaaggtgac cggacccgcg caggtgattg gcgggcgctc ctattgtgtc
     5941 ttcagctccg atgacggtaa ggcgaaagta ccgttcccgg cgacactttc ctttattacc
     6001 cgcaacggag ctacaaaaac ctacgatgcc gggtgcgatg atagctggcg ggatatgacc
     6061 gatgcgctgt ggttgaccac accgtggact gatatctctg gcgaagtggg gcagatggat
     6121 aagaccacag tcaaattttc gattccaatg gataacgcca tttcgctgcg tacggtagat
     6181 gataacggct ggtttggcga agtcagcgct tcaggagaga ttcatgttca ggcgacgtgg
     6241 cgtaacatta actaaggccc tgctgacagc ggtctgtatg ctggcggcac ctttgacaca
     6301 ggcgatttcg gtcggcaatc tgacattttc gctgccgtcc gagactgact ttgtcagcaa
     6361 acgtgtagtg aataacaaca aaagcgcacg gatataccgt attgccatca gtgctattga
     6421 tagcccgggc agcagtgaat tgcgcacccg accggtggat ggtgaactgc tttttgcccc
     6481 ccgccaactg gcgttgcagg ctggtgagag cgagtatttt aaattttact atcatggtcc
     6541 acgggataac cgcgagcgct actaccgggt ctcatttcgc gaagtcccca ctcgtaacca
     6601 gacaagacgt agcccaaccg gcggcgaggt cagcacggag ccggtggtgg tgatggatac
     6661 cattctggta gtacgaccac gtcaggttca gtttaaatgg tccttcgaca aggtgacagg
     6721 aacggtaagt aacactggca acacatggtt taagctactg attaagccag gatgtgattc
     6781 gaccgaagag gaaggcgatg cctggtatct acgtccggga gacgtggttc atcagcctga
     6841 gttacgtcag ccggggaatc attatctggt ctataacgac aaattcatta agattagcga
     6901 ttcctgtccg gctaagcccc cttcggcgga ctaagactta tccatcggtc gagggaagaa
     6961 ttccacctca gagctccaaa aatcgtttaa atgatgtgga agcgatcgtc aatgtcacct
     7021 gtaacgatgg gctttctcat atgcacacca tagtgttcag gagacacgga aaggtatcag
     7081 gcagcctgct aatgagtggt ttaatcacga attagtacgt aaaatcggta acggctggaa
     7141 atcattcaat actcacacta tcgaaagttc cccagccaac cgcggtacgt tcttacatac
     7201 gatgtaccgc tgttctcttt acgattttta gctgtactgg tgaattatga gcaatctgaa
     7261 tccatgcatg acgtgtggtg cctgttgtgc atttttccgc gtctcttttt actgggccga
     7321 agccgacgat gctggcggta ctattcccgc caggctcact gaacaaatat ccccttttca
     7381 ccgatgcatg agcggtacca atcagaaaaa cccccgatgt attgcccttg caggaacccc
     7441 gggcaaaaat gcctgctgca cgatatataa aaatcgatcg tccacatgca gagaatttgc
     7501 catgtctggt gagaacggag tcgtcaatga ggcttgcaat cgtgcaaggg ctaaatacgg
     7561 gctgacaccg ttataaacat acaataatta attgcactgc cccgccagcg ataatagcgg
     7621 ggcttcgttt ttcaggggta acaaaacccg atacttcttc tatttgccag caacaatgcc
     7681 tctcttctgt agcgttcttc ggatctacat catcctgaag tagcgcgata tactcactga
     7741 ctatctgaat cagaatataa aaa
//
LOCUS       DAZGLJ010000203         7757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000203 DAZGLJ010000000
VERSION     DAZGLJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.203"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..469
                     /gene="lpfA"
                     /locus_tag="ACHK49_003957"
     CDS             <1..469
                     /gene="lpfA"
                     /locus_tag="ACHK49_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long polar fimbrial major subunit LpfA"
                     /protein_id="HFO9264509.1"
                     /translation="AADAGDGTVKFTGEIVDAACVVSPDTQKQEVVLGQVNKSVFTAM
                     GDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKLVSVSTEPGAATGVGIGIYD
                     NTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTTGYGNAEVDFNLTYN"
     gene            553..1239
                     /gene="lpfB"
                     /locus_tag="ACHK49_003958"
     CDS             553..1239
                     /gene="lpfB"
                     /locus_tag="ACHK49_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO9264510.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            1263..3794
                     /gene="lpfC"
                     /locus_tag="ACHK49_003959"
     CDS             1263..3794
                     /gene="lpfC"
                     /locus_tag="ACHK49_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFO9264511.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3805..4860
                     /gene="lpfD"
                     /locus_tag="ACHK49_003960"
     CDS             3805..4860
                     /gene="lpfD"
                     /locus_tag="ACHK49_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFO9264512.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            4866..5390
                     /gene="lpfE"
                     /locus_tag="ACHK49_003961"
     CDS             4866..5390
                     /gene="lpfE"
                     /locus_tag="ACHK49_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO9264513.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5643..7334
                     /gene="eptB"
                     /locus_tag="ACHK49_003962"
     CDS             5643..7334
                     /gene="eptB"
                     /locus_tag="ACHK49_003962"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO9264514.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            7426..7502
                     /locus_tag="ACHK49_003963"
     tRNA            7426..7502
                     /locus_tag="ACHK49_003963"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7460..7462,aa:Pro,seq:cgg)
     gene            7537..7670
                     /locus_tag="ACHK49_003964"
     ncRNA           7537..7670
                     /ncRNA_class="other"
                     /locus_tag="ACHK49_003964"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         2018 a         1789 c         1974 g         1976 t
ORIGIN      
        1 cgcagctgat gcgggcgacg gtactgttaa attcaccggt gagattgttg acgccgcttg
       61 tgttgtgtct ccagacactc aaaaacaaga agttgttctg ggtcaggtga acaagtctgt
      121 atttactgct atgggtgata aatccacagc aacgccgttc aaaatcaaac tggaaaactg
      181 cgatatctcc actttcaaaa atgtggaaat cagctttaac ggcgttggcg atgcagacaa
      241 cagcaaactg gtttctgtaa gcactgaacc aggtgccgca actggcgtag gtatcggtat
      301 ttatgataac accaataccc tggttgacct gaatactggt aaatctgcta ctgtcctgaa
      361 agaaggtcag accgttctgt atttcaccgc taactatgtc tctaccaaag atgcagtaac
      421 cacgggttac ggtaacgctg aagtcgactt caacctgact tacaactaat cgatcctgac
      481 ggaacgatca tctctcaatg gcaacagaga ctgtgttgcc attattcaca ccaatgactc
      541 agggaccgaa tcatgaaacg tattagcttt gctgtgctgg cacttttaat gaccgcacaa
      601 cagtgttttg ctggtggcgt agccttaagc acgacgcgtg ttatttatga tggcggcaag
      661 aaagaagcgt cgctgaccgt tgaaaaccat aataaaaatg aagagttttt gatccagtca
      721 tggattgatg atgtgaatgg caataaaaaa acaccgttta ttattacacc gccattattc
      781 aaacttgatc ctgataaaaa taacgtctta cgtatcgtca atattgaaga taccttacct
      841 aaagatcgtg aatccgttta ctggattaat gtaaaggcca ttcctgcgaa aagtgctgaa
      901 aatgaaaata aaaacgtctt acagatagcc gttcgcaccc gtttgaagtt gttctatcgt
      961 ccaaaaggtt tgccgggaag caacctcgag gccatgaaac agttacgttt ccagcgtcaa
     1021 aataaccaaa ttgtggttaa taacgactcc gcttataacc tgacctttaa tcagttttcc
     1081 attaatggac aaaagattga acgggcggga atggttctcg cgaaaggaaa gttaaacatt
     1141 aaccttccag ccggtacagg caacgcgcac gaagtgaaat acagcattat taatgatttc
     1201 ggtgttgctg gcgaaatgct gacgaagccg attaactgat ccttttacaa ggcgctttta
     1261 ctatgcattg gatgcattta cctctctatc actacagagc gcatttttcc ttctctctgc
     1321 tggcattaac gattgccagc tcgttacctg cgtatggcgg aaaatttaac cctaaatttc
     1381 tcgaaaacgt tcagggtatc gatcaacaca tcgacttgtc ggtatacgac tcacctgtcg
     1441 gccagcaaat ccccggtaaa taccgggttt cggtgtttgt aaacgaagaa aaaatggctt
     1501 cccggacgct ggattttagt accgccagcg aagcgaaacg caaagccagc ggtgagtcgc
     1561 ttatgccttg ccttagccgg gtgcaactgg aagaaatggg cgtgcgcgtt gacagctttc
     1621 cggcgctgaa gatgtcacca ccagaagcct gcgtagcgtt tgatgaaatc attccccagg
     1681 ccaccagccg ctttgatttt aatactcaga cgctgcatct ttcctttcca caggcggcaa
     1741 tgatgatgac cgctcgcggc acggttgatc cttctcgctg ggacgaagga atacctgcgc
     1801 tgttgctgga ttacagcttt agtggcagca atggacgcaa tgaaggtaca ggttcatctt
     1861 cagattcgac cagtgacagt tattacctga acctgcgcag cggtttgaac gttggtccgt
     1921 ggcgtttacg taataacagt atctggaacc gtaccgatgg caaaaaccaa tgggataacg
     1981 ttggtacatc gttaaaccgg gcaattattc cacttaaatc acagataact ctgggtgata
     2041 ccgccacgcc tggcgagatt tttgatagcg tacaaatgcg tggggcgtta ctggcatccg
     2101 acgatgaaat gttgccggac agccagcgtg gctttgctcc ggtagtgcgc ggcatcgcca
     2161 aaagcaacgc tgaagtgagt atcgaacaga acggctatgt catttatcgc acttttgttc
     2221 agccgggtgc atttgagatt aacgatctct acgcaacctc cggcagcggc gacctgacgg
     2281 tgatcattaa agaagccgac ggaagtgaac aacgttttat ccagccgttt tcggcggtgg
     2341 cgattttcca gcgtgaaggg tatctgaaat acagtctggc tgccggtgaa tatcgtgctg
     2401 gtaattacga cagcgataaa ccacatttcg ggcagtttac cgccatgtac ggcttgccgt
     2461 ggggtatgac cgcctacggt ggtgcattgt tgtcggcaga ttacaacgct ttagctctgg
     2521 gcctggggaa aaacttcggt accattggcg cagtatcggt ggatgtcact caggcgaaaa
     2581 gtcagttacg caacaatgaa aaagaggaag gccagtctta tcgcttcctc tattcgaaaa
     2641 gttttgaggg tggcactgac ctgcgtctgt tgggctacaa atactcaacc agcggctact
     2701 acaccttcca ggaagcaact gatgttcgca gtgacgccga cagtgattac cgccgttatc
     2761 acaagcgcag ccagatacag ggcaatatta cccagcaact gggagactac ggctcggttt
     2821 atttcaatat gacgcagcag gattactgga acgttgacgg taaggaaaac tccttaagcg
     2881 ccggttatca cggtcacatt ggtcgggtga attacagcgt ggcctatacc tggacgagaa
     2941 gtccggaatg ggaagaagac gatcgcttgt ggtcattcag tgtttccatc cctctggggg
     3001 gcgcgtggag cagttatcgc atgactaccg accagaatgg caagacgtcg caacaggcct
     3061 ctgttagcgg cacgttactg gaagatcgta acctgagcta taacgttcag cagggttaca
     3121 ccagtaatgg cgtaggctac agtggcagcg tcaacatggg gtatatgggt ggctctggta
     3181 acattgacgt aggctataac tattctaaag ataaccaaca ggttaactat ggcgtgcgcg
     3241 gcggtgtcat cgttcacagt gaaggtataa cgctttcgca gcctttaggc gaaagtctgg
     3301 cgattgtttc tgcaccgggc gcgcgtggtg gacatgtggt taacagcagc ggcgtagaag
     3361 ttgactggat gggcaacgcc gtggtgcctt atctcacacc atatcgtgaa accattgtgg
     3421 aattgcgtag cgacacgttg gggcaaaacg ttgaactaca ggaagcgttc cagaaagtcg
     3481 tgcctactcg tggtgcagtt gtgcgttccc gctttgatac ccgcgtcggt taccgtgtgc
     3541 tgatgagctt aaaacaggcg aatggcaacg cagtaccgtt tggtgcaacc gcagcgttaa
     3601 ttgatgagag caaacctgcc agcagcattg tgggtgaaga aggacagctt tatatcagcg
     3661 ggatgccaga agagggcgaa ttgcaggtga gctgggggaa cgagcaggcg caacgttgcc
     3721 gtgtgccatt ccgcttgcca gagaacaagg acaatgcggc tatcgtgatg gttaatgcgg
     3781 tatgcgagaa ataaggatat ttagatgaaa attaaaaatt tgttggcagg tatggtgctg
     3841 ttagggacga gcacttttgc tggaaatata tgggctgcgg attggggacc atgtcagaca
     3901 tcaaatgggg tggcccatga gtatagtttt gatttcgtac agactatcca ggtaccttca
     3961 gaaaataagg ctgggaaaat attaactcag ccctttgctt taggtacaaa atattccgct
     4021 tattgtgaat gcccggatcc cattccagat aatggagttg tgacttattt taaaggtgta
     4081 acgcttctgc ctgagcctgg aactgtagat gggtactata aattcaataa ttacattgat
     4141 attctcacca aaatatatgt ctacacacaa ttagatatcc cggtgccttt taccgatcga
     4201 agcaatggca cggcacagaa agaatgtacg ccctatactg ctaacaattg ggggacaggt
     4261 ggtaaaggaa gtatcagtat ttatatttca catccttttg ttgggcaaat gattattcct
     4321 aaaacacgag ttgcgtccct ctttgggaca aaaaagaaag gtgtttacaa tgatagtcag
     4381 cccatggcca atgtcagtat ttccgggagt attacagtta ctcaaggctg tgaacttgcc
     4441 gctggcaccg tactggatat tccattcgga gaatatcagg cccatgattt taaagggcgt
     4501 gccggacaac caccacaaaa tgttcagaag gtacaaaaag aattaagctt tgactgcaat
     4561 aatatttcag atggcgtcaa aatttatctc agtattgatg caacaccaaa tactgcctat
     4621 cccagcgcaa ttgatttagg caacgccgat gttggtgcag tcattgagga cggtaaaggt
     4681 aatattctta agccgaatga cagtaatagc ttattagaaa tgaacccagg ctcgctgtat
     4741 gaagatgtca aacgcaaagc gacaaccaca attaccgctt atcccgttag cactaccgga
     4801 aaattacccg ctgccgggga ttatagcggt attgctacca tacatgtcga actggagtaa
     4861 cagtcatgaa gcgaacaata cttgcgttat gcctgctgcc tctttccttt tcgtcattcg
     4921 cggcaaccac ggatttaggg ccgaaaggaa cattgaaatt cacccttaaa atcagccagg
     4981 gtgcttgcga actggtgaaa gacagcgttg aggttgacat gggcaccgcg gttctcaaaa
     5041 agccggtaag aatcggaacg gaaattaatc ccactccttt ttccatcggc ctgaaaaact
     5101 gtagcgaagt ggtcagagcc tacgtgacta tggacggtac acccaatgca gacgactcaa
     5161 atctttttgc gctggatgcg ggtggcgcta caggcgttgg gctgaaaatc aaaaccgctg
     5221 ctggagtaca acagttcccg gaaaatacaa cgccgacacc ggttgagttt gcaattaacg
     5281 gtgatggcgc acatcaactg aactatatcg ccagctacgt gcctattcgc gccgatgcaa
     5341 ctattggtcg cgcagatgca acggtcgact ttactgtaca gtacgaataa tttcacatct
     5401 taagggccag ttaactggcc ctttttactg ctttttgtaa cgctaaagtc tcttttcaaa
     5461 cttgcatttt tgtaaatttg tgcttcatgc acactctttc cccacacttt ttcgctttgc
     5521 tgtggtctac ttattcgcgc gtgtagattt tacttatctg actacctccg cacttttccc
     5581 ctgccgggcc tgaaaagccg ctaagcaggg tgttatcacc tgtttgtcca gggtttgttt
     5641 gcatgagata catcaaatcg attacacagc agaagctgag ctttttgctt gcaatctata
     5701 ttggcctttt tatgaatggc gcggtttttt atcgccgctt cggcagctat gcgcacgatt
     5761 ttaccgtctg gaaaggcgtt tctgctgttg ttgaactggc cgccaccgta ctggtgacct
     5821 tctttttact acgtcttcta tcgctgtttg gccgccgcag ctggcgtatt ctggcgtcgc
     5881 tggtggtgct cttttccgca ggtgccagct attacatgac cttccttaat gtggtcattg
     5941 gttatggcat catcgcttcc gtcatgacca ccgatatcga cctgtcaaaa gaagttgttg
     6001 gtctgaactt tattctctgg ttaatcgccg ttagtgcatt gcctcttatc cttatctgga
     6061 ataaccgctg tcgctacacc ttgctccgac aactgcgaac cccagggcag cgtattcgca
     6121 gcctggcggt cgtcgtactg gcgggtatta tggtttgggc accgattcgt ttgctggata
     6181 tccagcagaa gaaagtggag agggcgaccg gcgttgattt gccgagttat ggcggtgtcg
     6241 tagcgaactc ttatctgcca tcaaactggc tttctgcgtt ggggctgtat gcctgggcgc
     6301 gggtcgatga atcttccgat aataattcat tgcttaatcc ggcgaagaaa ttcacttatc
     6361 aggcaccgca aaacgttgat gacacttatg tcgtgtttat catcggtgaa accacgcgtt
     6421 gggaccatat gggcattttc ggctatgagc gtaataccac gccgaaactg gcccaggaga
     6481 aaaatctggc ggcgttccgt ggttactcct gtgataccgc aaccaaactc tcactgcgtt
     6541 gcatgtttgt acgtcagggg ggcgcggaag ataatccgca gcgcacatta aaagaacaga
     6601 acattttcgc ggttctgaag cagttaggat tcagttctga cctctacgct atgcagagcg
     6661 aaatgtggtt ctacagcaac acgatggcgg acaacattgc ttatcgtgag cagattggtg
     6721 cggagccacg taatcgtggc aagccggtag atgatatgtt gctggtagac gaaatgcagc
     6781 aatcgctagg gcgcaacccg gatggtaagc atctgatcat tctgcatacc aaaggttcgc
     6841 attttaacta cacccagcgt tatccgcgta gcttcgcgca gtggaagccg gaatgtattg
     6901 gtgttgatag cggctgtacc aaagcgcaga tgatcaactc ctatgacaac tcggtgacct
     6961 atgtggatca ctttatctcc agcgtgattg atcaggttcg cgataagaaa gcgattgtgt
     7021 tctacgcagc tgaccacggt gagtcaatta atgaacgcga gcacctgcac ggcacgccgc
     7081 gtgaactggc accgccggag cagttccgcg taccgatgat ggtctggatg tcagataaat
     7141 atctggaaaa tccggccaat gctcaggcat ttgcgcagct gaaaaaagaa gccgacatga
     7201 aagtgccacg ccgtcatgta gagctgtacg acaccatcat gggttgtctt ggctatactt
     7261 caccggatgg tggaattaac gaaaacaaca actggtgtca catcccgcag acaaaagtgg
     7321 cagcggctaa ctaagcggcc tgctgacttt ctcgccgatc aaaaggcatt ttgctattaa
     7381 gggattgacg agggcgtatc tgcgcagtaa gatgcgcccc gcattcggtg attggcgcag
     7441 cctggtagcg cacttcgttc gggacgaagg ggtcggaggt tcgaatcctc tatcaccgac
     7501 caaattcgaa aagcctgctc agcgagcagg cttttttgca tttgaagccc atgaggatga
     7561 gaacctccgg gggcggggag gttcgactcg agcgaagcga gagaacgttg cgccagcaac
     7621 ggcccgcagg gcgagccacg aagtggcgag taatcctcta tcaccgacca aattcgaaaa
     7681 gcctgctcaa cgagcaggct tttttgcatt tgtagctcat gaggatgaga acctccgggg
     7741 gcggggaggt tcgactc
//
LOCUS       DAZGLJ010000204         7670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000204 DAZGLJ010000000
VERSION     DAZGLJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.204"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..4221
                     /locus_tag="ACHK49_003965"
                     /pseudo
     CDS             1..4221
                     /locus_tag="ACHK49_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            4444..4710
                     /gene="hipB"
                     /locus_tag="ACHK49_003966"
     CDS             4444..4710
                     /gene="hipB"
                     /locus_tag="ACHK49_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO9264515.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            4710..6032
                     /gene="hipA"
                     /locus_tag="ACHK49_003967"
     CDS             4710..6032
                     /gene="hipA"
                     /locus_tag="ACHK49_003967"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO9264516.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            6344..6552
                     /locus_tag="ACHK49_003968"
                     /pseudo
     CDS             6344..6552
                     /locus_tag="ACHK49_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            6984..7547
                     /gene="fimA"
                     /locus_tag="ACHK49_003969"
     CDS             6984..7547
                     /gene="fimA"
                     /locus_tag="ACHK49_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO9264517.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT         2100 a         1646 c         2061 g         1863 t
ORIGIN      
        1 gcgttcagga cggttcccac ggtgtcgtca acgtgaccga taaagggcat tggaatttcc
       61 tcggaacggg cgaagctttc cgctatatct atatcggtga tgctggcgac ggtgaactta
      121 atgtctcgcg tgaaggtaaa gtagattcgg gaattatcac tgcggggatg aaagaaacag
      181 gcacaggcaa cattactgtt aaggataaga actccgttat cactaatctc ggaactaatc
      241 ttggttatga cggccacggc gaaatgaata tcagtaatga agggcttgtt gtcagcaacg
      301 gaggaagctc actcggttat ggagaaaccg gcgtcgggaa tgtcagcatc accacggggg
      361 ggatgtggga ggtcaataag aatgtctata ccaccattgg tgttgcgggc gtcggaaacc
      421 tcaatattag cgatggcggt aagttcgtat cgcaaaatat tacttttttg ggcgataaag
      481 caagcggtat cggcacactg aacctgatgg atgcgacatc atcgttcgat actgtgggta
      541 tcaatgtcgg taattttggt agcggtatcg taaatgtcag taatggtgcc acccttaatt
      601 caacgggcta tggatttatc ggaggaaatg cctccggtaa ggggatagtt aatatttcaa
      661 cggacagtct ctggaattta aagacatcat ctactaacgc acaattgcta caggtcggtg
      721 tattaggcaa gggtgaactg aatattacca ccggaggtat agttaaagcg cgtgatacac
      781 agatagctct caatgacaaa agtaagggcg acgtgagggt ggatgggcag aactctcttc
      841 ttgaaacatt caatatgtac gtagggacat ctggtacggg tacgttaacc ctgacgaata
      901 gcggcacgct gaatgtcgaa ggtggagaag tttacttagg tgtttttgaa cctgctgtag
      961 gaacgctaaa cattggtgca gctcacggtg aggcggcggc agatgccggg tatataacca
     1021 acgcgacgaa agtagagttt ggttctggcg aaggtgtttt tgtctttaat catactaata
     1081 acagtgatgc cggctaccag gtcgatatgc tgattacagg tgacgataaa gacggaaaag
     1141 tgatccatga tgcaggccat acggtgttca atgcagggaa tacttatagc ggtaaaacgc
     1201 tggtcaatga cggcctcctg accattgcgt ctcatacggc agatggggta acgggcatgg
     1261 gttcgagtga agtcaccatt gcaagccccg gtacgctcga cattctcgca tcaacgaaca
     1321 gtgcaggaga ttacacgctg accaatgcgc tcaaaggcga tggcttgatg cgagtgcagc
     1381 tgtcatccta cgacaagatg tttggcttta cccatgcaac agggactgaa ttcgccggtg
     1441 ttgcccaact gaaagacagt accttcactc tggaacgcga caacaccgct gcgcttactc
     1501 acgcgatgtt gcagtctgac agtgaaaata ccacatcggt aaaagtggga gagcaatcca
     1561 ttggtggact ggccatgaat ggcggtacgc tcattttcga tacggatatt cctgctgcga
     1621 cgcttgcaga gggatatatc agcgtcgata cgctggttgt cggcgcgggt gactacacct
     1681 ggaaaggccg taactatcag gtaaacggga cgggcgacgt gcctaaaccg tggaatgatc
     1741 ccatggcgaa taaccctctg acgacgctca atttgctgga acacgatgat agccatgtcg
     1801 gcgttcaact ggtgaaggcg caaacggtta ttgggtcggg tggctcatta acgttacgtg
     1861 atttacaggg cgacgaggtg gaagcggaca aaacgttaca cattgcgcaa aacggaacgg
     1921 tggtcgccga gggtgattat ggattccgcc tcacgaccgc accaggtgat ggtttgtacg
     1981 ttaactatgg gctgaaagcg ctgaacatcc atggtgggca aaagctgacg ttagccgaac
     2041 atggcggagc ctatggcgca acggccgata tgtcggcaaa aatcggtggt gaaggggatc
     2101 tggcaatcaa tacggtgcga caggtttcgc tttccaacgg tcagaacgac tatcaggggg
     2161 caacctacgt tcagatgggg acattacgta ccgatgcgga tggtgcactt ggcaacaccc
     2221 gggaactgaa catcagcaac gcggccatcg tcgatcttaa tggatcgacg cagacggtag
     2281 agacattcac cgggcagatg ggttcgactg ttttgttcaa agagggggcg ctgacggtaa
     2341 ataaaggtgg gatcagtcag ggtgaactga caggtggcgg aaacctgaat gttacagggg
     2401 gaacgctggc tatcgagggg cttaatgcac gctacaatgc gttaaccagc attagcccaa
     2461 atgcggaagt cagcctcgat aatacgcagg ggttaggcag aggaaatatt gccaataacg
     2521 gtctgttaac gctaaaaaac gtgactggcg aactgcgcaa tagcataagc gggaagggta
     2581 tcgtgagcgc aaccgccagg acagatgtag agctggatgg cgataatagc cgctttgtgg
     2641 ggcaattcaa cattgataca ggcagcgcgc tcagcgtcaa cgagcagaaa aacctgggtg
     2701 atgcttccgt tatcaataat ggcctgctca ccatctccac tgagcgtagc tgggcgatga
     2761 cgcacagtat cagcggtagc ggtgatgtga caaaactggg taccgggatc ctgactctta
     2821 acaacgattc cgcggcgtat cagggtacga cggatatcgt gggggggggg gaaattgctt
     2881 tcggttccga ctctgccatt aatatggcaa gtcaacacat taatatccat aacagcggtg
     2941 tgatgtcggg aaatgtcacc actgcaggtg atgtgaacgt tatgcctggg gggacactgc
     3001 gtgtcgctaa aaccacaatc ggcggcaacc tggagaatgg cggcacggtt caaatgaata
     3061 gcgaaggggg gaaaccgggg aatgtactga ccgttaacgg caactatacc ggaaacaatg
     3121 gcctgatgac gttcaacgcg acgctgggcg gcgataattc gcccaccgat aaaatgaacg
     3181 tgaaaggcga tacccaaggg aacactcgcg ttcgggttga taacattggc ggcgtcggtg
     3241 cacaaacggt caacggtatt gaactcattg aggttggcgg taattctgca ggtaacttcg
     3301 cgctgaccac cggaactgtc gaagctgggg cttacgtcta cacgctggct aaagggaagg
     3361 ggaatgacga gaaaaactgg tatctgacca gtaaatggga cggcgtaacg ccagcggata
     3421 cacccgatcc catcaataat ccccctgttg tggatccgga aggcccatca gtttatcgcc
     3481 cggaggccgg aagctatatc agcaacattg ccgcagccaa ctcgctgttt agccatcgtt
     3541 tacacgaccg tctgggtgag ccgcagtata tagattcact gcattctcag gggtcggcaa
     3601 gcagtatgtg gatgcgtcat gtcggagggc acgaacgttc aagggccggt gacggtcagc
     3661 taattactca ggctaaccgc tatgtattgc agctaggcgg cgatttggcg cagtggagta
     3721 gcaacgcgca ggatcgctgg catgacggcc tctgttgagg gtgggtatac ctttgaagcg
     3781 ggaacattta gaggcagcga agggacgctg aatacctggt acgtccagcc acaggcgcaa
     3841 atcacctgga tgggtgtgaa agattctgac cataatcgga aagacggaac acgcattgaa
     3901 acggaaggcg acggaaacgt gcaaacgcga cttggggtga aaacctacct gaatagccat
     3961 cacaagcgtg acgatggtaa acagcgtgag ttccagcctt acattgaagc gaactggatc
     4021 aacaatagca aagtctacgc cgtgaagatg aatggtcaaa ccgtaagccg tgatggtgcg
     4081 cgtaatctcg gtgaagtacg taccggggtt gaggcgaaag taaataacaa ccttagcctg
     4141 tgggggaatg tcggtgtgca actaggtgat aaaggctata gcgatactca gggcatgctg
     4201 ggagtgaaat atagctggta aaccgtataa gccgcatgtc gagatggcat gcggcttaat
     4261 attgccgact tcaaacggcg catcagcgcc ttttttaaat cctccttttt atccgcgatc
     4321 gcggatatcg cagcgtttat cccgtagagc ggataagacg tgtttccaaa ttgacttatc
     4381 ctcactaaag gataaaactg ataatatcct cttatgcgga taaatttgct gtggacgtat
     4441 gacatgatga gctttcagaa gatctatagc ccaacgcaat tggcgaatgc aatgaaactg
     4501 gttcgccagc aaaatggctg gacgcagagc gagctggcga aaaaaattgg cattaagcag
     4561 gcgacaattt ccaatttcga aaacaaccct gacaatacca cgctcacgac attttttaag
     4621 attttacagt cgcttgaact ctcaatgaca ctatgcgaca cgaaaaatgc ctcgccagaa
     4681 tcaacagaac agcaagatct ggagtggtaa tgcctaaact tgtcacttgg atgaacaacc
     4741 agcgggtagg cgagttaacg aagttagcca acggcgcgca cacctttaag tatgcaccgg
     4801 agtggttagc cagccgttat gccagaccgt tgtcactttc gctgccattg cagaggggga
     4861 atatcacctc agatgccgta tttaacttct tcgataacct gttacccgat agcccgattg
     4921 tacgtgaccg gatcgttaaa cgttatcatg ctaaatccag acaaccgttt gatttattgt
     4981 cagaaatagg gcgagacagc gttggtgccg taacgttatt acccgaaaat gaaaccataa
     5041 cgcgcccgat aatggcatgg gaaaagctta ctgaagccag gcttgaagaa gtattaacgg
     5101 cttataaagc agatatccct ctgggcatga ttagagaaga aaatgacttt cgcatctcgg
     5161 ttgctggcgc gcaggagaag acagcgctgc ttagaatagg caatgactgg tgcattccga
     5221 aaggaataac gccgacgacg cacatcatta aattaccgat tggcgaaatc agacagccca
     5281 atgcgacgct cgatctcagc caaagcgttg ataatgagta ttactgtctg ctgctggcga
     5341 aagaacttgg gttgaatgtt ccggacgcag aaatcattaa agcgggaaat gtgcgcgcgt
     5401 tagcggtcga acgttttgac aggcgttgga atactgagcg aacggtttta cttcgcttgc
     5461 cacaggagga tatgtgtcag acattcggtt taccttcatc tgtgaaatat gaatcagatg
     5521 gaggcccagg catcgcgcgg atcatggcgt ttttgatggg gtccagcgag gcgcttcgcg
     5581 atcgttatga ttttatgaaa ttccaggtct tccagtggtt gattggcgca acggatggcc
     5641 atgcaaaaaa cttctccgta tttattcagg ctggcggcag ttatcgactc acgccatttt
     5701 acgacatcat ttcagcattt ccggtccttg gcggtacggg aatacacatc agcgatctca
     5761 aactggcaat ggggcttaac gcatccaaag gcaaaaaaac ggcaatcgat aaaatttatc
     5821 cgcgacattt tttggcgaca gcaaaggtgc tgagattccc ggaagtgcag atgcatgaaa
     5881 tcctaagtga ctttgccaga atgattccgg cagcactgga taacgtgaag acttcattac
     5941 cgacagattt tccggagaac gtggtgacgg cagttgaaac caatgtgttg aggttgcatg
     6001 gacggttaag ccgagaatac ggtagtaagt gagatatggg gtttggtcgt tgttaatgag
     6061 cacgacaatc atgaccgcca acgactggat tcttccttgg tttatactgg tgcgcataat
     6121 gtatattatg ttaaattatg tctgagtgat gcgaagtgct tttctggtag tcgttattcg
     6181 ttcagggttc aagaacttaa gcctctcctc tttcataatt ggcctgtgca tggagaaata
     6241 gcctggttat tccctgcccc atgctaagag cgcagtgtct gtcgcataga cactgcatac
     6301 gaaaacacca gagggtgctg ttacttattt aaggacggat caagcgggaa tgagtccact
     6361 ccggtatcag aaatggctac gtctcaatga agtcaggcga ccgatgctga atgaacatta
     6421 cgatgtcacc acagcagcct atgctgtcgg ttacgaaagc tatcccattt cagtcgggaa
     6481 tattcgcgga tgtttggaga gtcacccaag agagatatta ccaggttaag aaagtctgtc
     6541 ggtcagcttt aataaaccct gcacttatct gtttagagca attcggtgtt agtttcagca
     6601 agcaaacatt aaccatagct aatgatttat agccatatta accattggta gcccttgacc
     6661 tttcctgaca cttcccttgt tcgcaataat attctgttta gtcacggttt ataaataaca
     6721 tgcataatga aatcgtcatg ttatttagcg acataaaatg tttttgcaaa tactctcttc
     6781 aacaagtgca caataaactt gttgtgatta ggtgtccttg tgatttaaag atattatcgt
     6841 tcttcgtgaa atgtaataac cctggaaact atattctcag ggcaacaacc aatcaagaat
     6901 gattcttgtt tttaagcaat aacagaatga cttaattgtt aaccctcttt attcagctac
     6961 ttaaatataa attttggaga attatgaaac tcaaacatgt tggtatgatt gtcgtttctg
     7021 tgttggcgat gtcgtctgct gcggtaagcg cagccgaggg tgatgaatca gtaacgacca
     7081 ctgttaatgg cggtgttatt cattttaaag gtgaagtggt aaatgccgct tgtgcaattg
     7141 attcagaatc aatgaaccaa acggttgagc tgggtcaggt tcgttcttct cgcttggcta
     7201 aagcgggtga cctcagctcc gccgttggct tcaatatcaa gctgaatgat tgtgatacca
     7261 atgtttccag taatgcagct gttgcattcc tgggtactac tgtcaccagt aatgacgata
     7321 cgttagcgct gcagagttca gcggcaggct ctgcccaaaa tgtcggtatt caaattttgg
     7381 accgtacggg tgaggtatta atacttgatg gggccacttt tagtgctaaa accgacttga
     7441 ttgatggcac gaatatactg ccattccagg ctcgttatat tgctctcggg cagtccgtag
     7501 ctggtactgc aaacgctgat gcgaccttca aagttcaata tctataattc aatcttaagg
     7561 tggctatacg tcatctgaaa ttgaaacagg gtgtgtgtgg catggctacg cacacccgag
     7621 caatttacca atattcatct catttttaat gagaaatggt ttgtatttgg
//
LOCUS       DAZGLJ010000205         7651 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000205 DAZGLJ010000000
VERSION     DAZGLJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.205"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..565)
                     /gene="ppiA"
                     /locus_tag="ACHK49_003970"
                     /gene_synonym="rotA"
     CDS             complement(<1..565)
                     /gene="ppiA"
                     /locus_tag="ACHK49_003970"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO9264518.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKV"
     gene            836..2017
                     /gene="tsgA"
                     /locus_tag="ACHK49_003971"
     CDS             836..2017
                     /gene="tsgA"
                     /locus_tag="ACHK49_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO9264519.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            2279..4822
                     /gene="nirB"
                     /locus_tag="ACHK49_003972"
     CDS             2279..4822
                     /gene="nirB"
                     /locus_tag="ACHK49_003972"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO9264520.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            4819..5145
                     /gene="nirD"
                     /locus_tag="ACHK49_003973"
     CDS             4819..5145
                     /gene="nirD"
                     /locus_tag="ACHK49_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO9264521.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            5271..6077
                     /gene="nirC"
                     /locus_tag="ACHK49_003974"
     CDS             5271..6077
                     /gene="nirC"
                     /locus_tag="ACHK49_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO9264522.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLSNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            6096..7469
                     /gene="cysG"
                     /locus_tag="ACHK49_003975"
     CDS             6096..7469
                     /gene="cysG"
                     /locus_tag="ACHK49_003975"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO9264523.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         1774 a         1952 c         2028 g         1897 t
ORIGIN      
        1 ggactttagc ggaaaggata actaccggtt ttgacggcac attctggtac ggaccaacgt
       61 catgagtcgg cacctgggaa atcttatcgg caacgtccat gcctttcacc actttaccaa
      121 ataccgcgta accgaaatca cgctgaccat ggtcaaggaa ggcgttatcg gcaacgttga
      181 taaagaactg gctggtggcg ctgtctttgt cagcggtacg cgccatcgcg atggtgccac
      241 gcgtgttgcg caggccgtta tcggcttcat ttttgattgg cgggtttggt tttttctgct
      301 gcatctgctc ggtgaaaccg ccgccctgaa tcataaagcc aggaatgacg cggtgaaagg
      361 tagtgttgtt ataaaaaccg ctgttcacat aatcgacaaa gttttgcaca gacactggcg
      421 ctttttgttt atccagctcc agttcgatgt taccagctga ggttgtcaac aatacgtgcg
      481 ggtccccttt cgctgccatt gctgcgggag aaagagcaga aagagcgaaa acagcagcca
      541 tcgccgccag ggtcgatttg aacatgagat ttccttaaca aacagagcag aaaaaaagca
      601 agtgaagaga ttctaaatag ccttcaagaa caaggccatc cctttaccta attttacgta
      661 tctgaaatat atgtaacctg acgatcactt tttattccgc gatcaaaatc acctcttaaa
      721 atgcaattta gcaaccgatt gcaataaaac atttaaacag atcacaaaat cacctaaaat
      781 cgcccgctcg cggcatatcc cgctatgcca ttttattttt gaacgcaggc caatcatgac
      841 taacagcaat cgcatcaagc tcacatggat tagctttctc tcctacgcac tgactggtgc
      901 gttggttatt gtcaccggga tggtgatggg aaatatcgcc gattatttca atctgcctgt
      961 ttccagtatg agtaacacct tcaccttcct caacgccggc attttaatct ctatcttcct
     1021 caacgcctgg ctgatggaaa tcgtcccgtt gaaaacgcag ttacgttttg gctttctcct
     1081 gatggtgctg gcggttgccg gtttgatgtt cagccacagc ctggcgctgt tctcggcggc
     1141 gatgttcatt ctcggggtgg tcagcggcat caccatgtcg attggtacat tcctggtaac
     1201 acaaatgtat gaagggcgtc agcgcggttc ccgcctgtta tttaccgact ccttcttcag
     1261 tatggctggg atgattttcc caatgatcgc cgcgtttcta ctggcgcgca gcattgagtg
     1321 gtactgggtt tatgcctgca tcgggctggt gtatgtcgct atttttattc tgaccttcgg
     1381 ctgtgagttc ccggcgctgg gcaaacatgc gccaaaaacg gatgctccgg tagagaaaga
     1441 aaagtggggg atcggcgtac tgtttctctc tgttgcggcg ctgtgctaca tcctcggtca
     1501 gttaggtttt atctcctggg tgcctgagta tgccaaaggc ctgggcatga gcctgaacga
     1561 cgcgggcacg ctggtgagta acttctggat gtcatacatg gtcggcatgt gggcgttcag
     1621 ctttattctt cgcttctttg atttgcaacg cattctgacc gtactggctg gtctggctgc
     1681 gattctgatg tacgtcttta acaccggaac accagcacat atggcgtggt caattctcgc
     1741 tctgggcttc ttctccagcg cgatctatac caccatcatc accctgggtt cacagcagac
     1801 caaagtaccg tcgccaaaac tggttaactt tgtcctgacc tgcggaacca tcggtactat
     1861 gttgaccttt gtggttaccg gcccgattgt tgaacatagc ggtccgcagg cggcactgct
     1921 tacagcaaac ggtctgtacg ctgtcgtctt tgtgatgtgc ttcctgttag gtttcgtcag
     1981 ccgtcaccgt cagcataaca ccctgacctc tcattaattg ctcatgccgg acggcactct
     2041 cgtcgtccgg ccttttcctc tcttcccccg ctacgtgcat ctatttctat aaacccgttc
     2101 attttgtcca ttttttgcac aaacatgaaa tgtcagacaa ttccgtgact taagaaaatt
     2161 tatacaaatc agcaatatac cccttaagga gtatataaag gtgaatttga tttacatcaa
     2221 taagcggggt tgctgaatcg ttaaggtagg cggtaataga aaagaaatcg aggcaaaaat
     2281 gagcaaagtc agactcgcaa ttatcggtaa cggtatggtc ggccatcgct ttatcgaaga
     2341 tcttcttgat aaatctgatg cggccaactt tgatattacc gttttctgtg aagaaccgcg
     2401 catcgcttat gaccgcgtac acctctcgtc ttacttctct caccacaccg ccgaagagct
     2461 gtcgctggtg cgcgaaggct tctacgagaa acacggcatc aaagttctgg tcggcgaacg
     2521 cgctatcacc atcaaccgtc aggagaaggt gattcactcc agcgccgggc gcaccgtttt
     2581 ttatgacaag ctgatcatgg cgaccggttc ctacccgtgg atcccgccaa tcaaaggttc
     2641 tgatactcag gactgctttg tctatcgcac tattgaagac ctcaacgcca ttgaatcctg
     2701 cgcccgtcgc agcaaacgcg gtgccgttgt tggtggcggc ctgttaggtc tggaagccgc
     2761 tggcgcgctg aaaaacttag gtattgaaac ccacgttatc gaatttgccc ctatgctgat
     2821 ggcggaacag cttgatcaga tgggtggcga gcagctgcgt cgcaaaatcg aaagtatggg
     2881 cgtgcgcgtt cacaccagca aaaacaccct tgagattgtg caggaaggtg tcgaagcgcg
     2941 taaaaccatg cgttttgccg acggcagcga actggaagtc gactttatcg tcttctctac
     3001 cggtatccgt ccgcgcgata agctggcaac ccagtgtggt ctggacgttg ctccgcgtgg
     3061 gggtatcgtc attaatgatt cctgccagac ttccgatccg gatatctacg ccatcggtga
     3121 atgcgcaagc tggaacaacc gtgtatttgg tctggtagca cctggctaca aaatggcgca
     3181 ggtcgccgtt gaccatattc tcggtagcga aaacgccttt gaaggtgctg accttagcgc
     3241 caagctgaaa ctgctgggcg tagacgtagg cggtattggt gatgcgcacg gtcgcacgcc
     3301 tggcgcacgt agctacgttt acctcgacga aagtaaagag atctacaaac gcctgattgt
     3361 cagcgaagac aacaaaaccc tgctcggtgc ggtactggtg ggcgatacca gcgactacgg
     3421 caacctgctg caactggtgc tgaatgctat cgagctgccg gaaaacccgg attctctgat
     3481 tctgccggcg cactcgggta gcggtaagcc gtctatcggt gttgataaac tgccggacag
     3541 cgcgcaaatc tgctcctgct tcgacgtcac caaaggtgat ctgattgctg ccatcaacaa
     3601 aggctgccac accgttgcgg cgctgaaagc tgaaaccaaa gcgggtactg gctgcggtgg
     3661 ctgtatcccg ctggttactc aggtactgaa cgcggaactg gcgaaacagg gcatcgaagt
     3721 taacaacaac ctgtgcgaac actttgctta ttcgcgtcag gaactgttcc atttgatccg
     3781 cgttgaaggc attaaaacct tcgaagaact gctggcgaaa cacggcaaag gctacggttg
     3841 tgaagtttgt aaaccgaccg tcggttcgct gctggcctcc tgctggaacg aatacattct
     3901 gaagccggaa catactccgc tgcaggattc taacgacaac ttcctcgcta acatccagaa
     3961 agacggcacc tactcggtga tcccgcgttc tccgggcggt gaaatcaccc cggaagggct
     4021 gatggcagtg ggtcgtatcg cgcgtgaatt taatctctac accaaaatca ccggctccca
     4081 gcgcctggcg atgtttggcg cacagaaaga cgatctgccg gaaatctggc gtcagctgat
     4141 tgaagctggc ttcgaaaccg gtcatgccta tgcgaaagca ctgcgtatgg cgaaaacctg
     4201 cgtgggtagc acctggtgcc gctacggtgt tggcgatagc gtcggcctcg gcgtggaact
     4261 ggaaaaccgc tacaaaggca tccgtacgcc gcacaaaatg aagttcggtg tctccggctg
     4321 tacccgtgaa tgttcagaag ctcagggtaa agacgtgggt attatcgcca ctgaaaaagg
     4381 ctggaacctg tatgtttgcg gtaacggcgg catgaaaccg cgtcatgcgg atctgctggc
     4441 agcggatatc gatcgcgaaa cgctgatcaa atatctcgac cgcttcatga tgttctacat
     4501 ccgtaccgcc gacaaactga cgcgtaccgc gccgtggtta gaaaacctcg aaggcggcat
     4561 cgattacctg aaagcggtga tcattgacga caaactgggg ctgaacgctc atctggaaga
     4621 agagatggcg cgcctgcgtg aagcggtagt gtgtgagtgg actgaaacgg tcaatacacc
     4681 gtccgcgcag actcgcttca aacacttcat caacagcgac aagcgtgacc cgaacgtgca
     4741 gatggtgcca gagcgcgaac agcaccgtcc ggcaacgccg tatgaacgta tcccggtaac
     4801 tctggtggag gacaacgcat gagccagtgg aaagacatct gcaaaatcga tgacatcctg
     4861 cctgaaaccg gcgtctgcgc gctgttaggc gacgagcagg tcgcgatttt ccgcccgtat
     4921 cacagcgacc aggtgtttgc gatcagcaac atcgatccgt tcttcgagtc cagcgtgctg
     4981 tcacgcgggc tgattgcgga acaccagggc gagctgtggg tcgccagccc gctgaaaaaa
     5041 cagcgttttc gcttaagtga tggcttgtgc atggaagacg aacagttttc cgtcaaacat
     5101 tacgaagcac gagtgaaaga cggcgtggtg cagctgcgcg gttaatgttt taacgggagg
     5161 cgcaatgcct cccctttttg catggtcctg taatgacctt cggtataatg caggacattt
     5221 tttaaacttt ttgttttatt ttttgttttt attttttaaa ggataatcaa atgtttacag
     5281 acactattaa taagtgtgcg gctaatgctg cacgcattgc acgcctgtcg gcaaataacc
     5341 cgctcggctt ttgggtcagc tccgccatgg cgggcgcata tgtgggtctt gggatcatcc
     5401 tgattttcac gctcagtaat ttgctcgacc cgtccgtacg tccgctggtg atgggcgcga
     5461 cctttggtat cgccttaacg ctggtgatta tcgccggttc tgaactgttc accggacaca
     5521 ccatgttcct cacctttggg gtaaaagcgg gcagcatcag ccacgggcaa atgtgggcaa
     5581 tcctgccgca aacctggctg ggtaacctgg tcggttccgt cttcgttgcc atgctctata
     5641 gctggggcgg cggtagcctg ctgccggtag ataccagcat cgttcactcc gtcgcgctgg
     5701 ctaaaaccac tgcaccggca atggtactgt tcttcaaagg tgcattgtgt aactggctgg
     5761 tttgcctggc aatctggatg gcgctgcgca ctgaaggggc ggcgaaattt atcgctatct
     5821 ggtggtgtct gctggcattt atcgcgtccg gctacgagca ctctatcgct aacatgacgc
     5881 tgttcgcgct ctcctggttc ggcaaccaca gcgaagccta cacgctggcg ggtattggtc
     5941 ataacctgct gtgggtgacg ctgggtaata ctttatcagg tgccgtattc atgggattgg
     6001 gttattggta tgctacgccg aaagcgaatc gtccggttgc ggacaaattt aatcaaactg
     6061 aaacggctgc cggttaatta ctaaggggtt tttacgtgga tcatttgcct atattttgcc
     6121 aattacgcga tcgcgactgt ctgattgtcg gcggtggtga tgtcgcggaa cgcaaagcaa
     6181 ggttgctgtt agacgcaggc gctcgcttaa cggtgaatgc attagcgttt attccacagt
     6241 tcaccgcatg ggcagatgca ggcatgttaa ccctcgtcga agggccattt gatgaaagcc
     6301 ttctcgacac ctgctggctg gcgattgcag cgacggatga tgacacgctt aaccagcgcg
     6361 tcagcgaagc cgctgaagct cgtcgcatct tctgtaacgt agtcgatgcg ccgaaagccg
     6421 ccagctttat tatgccgtcg attattgacc gctcaccgct aatggtagcg gtctcctctg
     6481 gcggcacctc tccggttctg gcgcgcctgt tgcgcgaaaa actcgaatca ctgctgccgt
     6541 tgcatctggg ccaggtggcg aaatatgccg ggcaattacg cgggcgagtg aaacaacagt
     6601 tcgccacgat gagtgagcgt cgccgtttct gggagaaatt gttcgttaac gaccgcctgg
     6661 cgcagtcgct ggcaaacaac gatcagaaag ccattactga aacgaccgaa cagttaatca
     6721 acgaaccgct cgaccatcgc ggtgaagtgg tgctggttgg tgcaggtccg ggcgatgccg
     6781 ggctgctgac gctgaaagga ctgcaacaaa ttcagcaggc agatgtggtg gtctacgacc
     6841 gtctggtttc tgacgatatt atgaatctgg tacgccgcga tgctgatcgc gttttcgtcg
     6901 gcaaacgcgc gggataccac tgtgtaccgc aggaagagat taaccagatc ctgctgcggg
     6961 aagcgcaaaa aggcaaacgc gtggtgcggc tgaaaggtgg cgatccgttt atttttggcc
     7021 gtggtggcga agagctggaa acactgtgca acgcgggtat tccgttctcg gtggttccgg
     7081 gtattaccgc agcttctggt tgctctgcct attcgggtat tccgctcacg catcgcgatt
     7141 atgcccagag cgtgcgctta attaccggac acttaaaaac cggtggcgag ctggactggg
     7201 aaaacctggc ggcagaaaaa cagacgctgg tgttctatat ggggctgaat caggctgcga
     7261 ctattcagca aaagctgatt gaacacggta tgcctggcga aatgccggtg gcaattgtcg
     7321 aaaacggaac agcagtcacg cagcgcgtga ttgacggtac gctcacgcaa ctcggtgaac
     7381 ttgctcagca aatgaacagt ccatcgctaa ttattattgg tcgggttgtt ggcctgcgcg
     7441 ataaactgaa ctggttctcc aaccattaat ttaacccggg ccagagaatt ctggccttct
     7501 taatgactct ttttatatat tcagcaaata aaacatagcc ccttaataaa tattatctgc
     7561 taatgggtta tattcaccgt tacagcgact tacaaaatta agtcattcgc tcactgacgc
     7621 caattaaata ttcctgtatc gttcttaacg a
//
LOCUS       DAZGLJ010000206         7650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000206 DAZGLJ010000000
VERSION     DAZGLJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.206"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..63)
                     /locus_tag="ACHK49_003976"
     CDS             complement(<1..63)
                     /locus_tag="ACHK49_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264524.1"
                     /translation="MSQNLDATAINQIHALISAQG"
     gene            complement(129..491)
                     /gene="yfdP"
                     /locus_tag="ACHK49_003977"
     CDS             complement(129..491)
                     /gene="yfdP"
                     /locus_tag="ACHK49_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO9264525.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            complement(1158..1832)
                     /gene="ymfK"
                     /locus_tag="ACHK49_003978"
     CDS             complement(1158..1832)
                     /gene="ymfK"
                     /locus_tag="ACHK49_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HFO9264526.1"
                     /translation="MKTVHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            1923..2123
                     /gene="ymfT"
                     /locus_tag="ACHK49_003979"
     CDS             1923..2123
                     /gene="ymfT"
                     /locus_tag="ACHK49_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO9264527.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            2167..2718
                     /gene="ymfL"
                     /locus_tag="ACHK49_003980"
     CDS             2167..2718
                     /gene="ymfL"
                     /locus_tag="ACHK49_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO9264528.1"
                     /translation="MGKYHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAAGTHYIADAVAQSAGGVFVSLPEIEEVENADINQR
                     LLEVIEQIGNYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            2864..3043
                     /locus_tag="ACHK49_003981"
     CDS             2864..3043
                     /locus_tag="ACHK49_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9264529.1"
                     /translation="MQELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEGPPAH"
     gene            2973..3866
                     /locus_tag="ACHK49_003982"
     CDS             2973..3866
                     /locus_tag="ACHK49_003982"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO9264530.1"
                     /translation="MSALVLCGNSGVILLSVKDHQHIDSAIPGRYTVQAPYKAGAGRG
                     NPEFTKAHNRALAVFLCHEQHYAQIMVGRAGPTSVGPGSLVTGISTPVRLTTNKVVES
                     LGGELLKITKEAAIMATIPTLTQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRTL
                     ECSASFTARNFSVSDYTDCTGRKLPCYQITRDGFAFLAMGFTGKRAAQFKEAYINAFN
                     QMEKQLSKLAVPSDVAHNASVLYSYISSIHQVWLQQLYPMLEKAESPLAVSLYDRIND
                     AAFLARLIHSSLNSSEVRGRK"
     gene            3863..4087
                     /locus_tag="ACHK49_003983"
     CDS             3863..4087
                     /locus_tag="ACHK49_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264531.1"
                     /translation="MIRNIFKRFTNQTFRCPRPGQWYTTPAGHVLRVSLVDRECQKVI
                     CEPLGRNYRVSMPLIAFRSGKNMKHLGGAA"
     gene            4084..4902
                     /locus_tag="ACHK49_003984"
     CDS             4084..4902
                     /locus_tag="ACHK49_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9264532.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSLGGSANNSL
                     SGAANSPGSAGVAPGGSAGDSPRTSHSFEPVKEPVNEPIAVGASADESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVNPETMLEGVKRYAGWVSAMGNSG
                     TQFVKQAVTFFGPDRHFEESWEVPAVSAARRDDPYFKASYDNVDYSQIPAGFRG"
     gene            4899..5393
                     /gene="yfdN"
                     /locus_tag="ACHK49_003985"
     CDS             4899..5393
                     /gene="yfdN"
                     /locus_tag="ACHK49_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO9264533.1"
                     /translation="MIMSLLNDVQKFIEAHPGCASGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRQTEVLQESEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            5393..6046
                     /locus_tag="ACHK49_003986"
     CDS             5393..6046
                     /locus_tag="ACHK49_003986"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO9264534.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASSMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            6043..6369
                     /locus_tag="ACHK49_003987"
     CDS             6043..6369
                     /locus_tag="ACHK49_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO9264535.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLAGEENARLRATHWLH
                     ERGLKV"
     gene            6366..6755
                     /locus_tag="ACHK49_003988"
     CDS             6366..6755
                     /locus_tag="ACHK49_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9264536.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISEAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPKGKVEITISKYEKPAGAAA"
     gene            6775..7584
                     /locus_tag="ACHK49_003989"
     CDS             6775..7584
                     /locus_tag="ACHK49_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO9264537.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGGEQKKRPKYWLSNK
                     QTCELIEQLFTEGGIPPLEKNQPVSVINGGNNQGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSASEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
BASE COUNT         1907 a         1819 c         2033 g         1891 t
ORIGIN      
        1 accctgagca gaaataaggg catggatttg attaattgcg gttgcgtcta agttctgaga
       61 cataataagt cctcactata taaagatatt cagtgatgag ataaataatc agttaattaa
      121 gaacgatatt aatgacctgc tgcgcggagt tttccgtcag gttcaccggc aagagtcagt
      181 aattgtccct ggtcttcctg cagaatagtc agtcgaccac cgcgattgac atacatcggc
      241 gtttcggtgg tgtcttcttc ggaaattttc ccgcggttag tcgggcgaac atatgagagt
      301 ttgtgtttga ttttcacacg gttctcatca aacggttcga tttccaggtt gagcgagacc
      361 ttacctttgg ttttcgtgtt catcacaccg gaagcgactt cactgagaac tgcgccgatt
      421 ttggtttcaa atacgccgcc gtccagctcc ccgataaatg cctgcacatc agtactgcgt
      481 tcgctagcca ttttgctgct cctcatcata tcgaccctgc aaggtcggtt ggtttctcca
      541 caaaacagag aagaacacct gcggtggcag ccgcccggct ggattgggtt atgagcccgt
      601 cgtccggtga tgctcttctc tgttttgtaa aaagagcggt accagccgga agcaagtgta
      661 caaactggta ccgccaaagc agtggctgtt gtggtggggt tgtcactcag gcgtatggtc
      721 aacctgacaa tccggtgtcc tcaacgggga aagagtaacc ccgccatact taccgccgcg
      781 ccatttcgcg gattaccaca acgctgagag cacttagcaa gttacggcac cacactttgt
      841 cgcggttcca taaatgccct catcgttgca ccctggtctc ttcccaggcg tcaaaccgga
      901 tcgccgcgct ggttaggcgt cttatcagca tcatcattga cttgcacatt ccggctacct
      961 ggtttttttg cccgagcaag gagtggattg tcccctttaa cgtccccaga ccgctaacga
     1021 cgcatgtgcc atacgccgtg ttacaaccaa attttgttta atcttgcctg cggcatgttt
     1081 cttttagata cattatgtat ctcaagggta cattgtcaag tataaaaaaa cctgccgaag
     1141 caggttataa atattgatta ggcctttatt ttgtatcttc ttggttttcc tgagaaaatt
     1201 actgtaccaa ttatagagca attaccgtta atcttaatgt aaggctcagg ccagtttggg
     1261 tttaatgctt tgagataacg ctgtgttcca tcttctatca atcgcttgaa ggtggtttcg
     1321 cctgtatcgt gcatcaatgc aataacgtcg tcaccgtggc aggcagggac ttcgggatca
     1381 acaaaaatca tgtctcccgg gcggtactca tcaatcattg aatcaccaat cacccgcaag
     1441 atataagtca tttcgccaca gggtacaggg cagggataag tttctgctgt gctcaaatca
     1501 acctcagaat agccaacttc tttccatgct ccggcctgta cccatgatat gacagggact
     1561 aacgttattt gtttgttagt gattgaaaca tcaggttttt ttgtgatgtt cgtggtctgg
     1621 tgttcttgat caagccatcc gacaggcagg tcgaaacatt tttcgatgtg ccgtgccatg
     1681 ctgtcaccga tatttttagt agcaccatct cccataaacc tgctggtctg ggttggctcg
     1741 cgatcaatca tggtggcaaa ggaagaattc ccgccaacac catctctcag ttttctggcg
     1801 ttagaccgcc ggatgtcatg gactgttttc ataaagaaat taaaaccttt gtaccgataa
     1861 ggtacaagta tcttgaaggt tcatattaat catgtaatat gtatattgga ggtacatatt
     1921 gtatgaaagc gtattgggac tctttaacca aagaacagca gggcgagttg gccggaaaag
     1981 ttggctcaac accaggctac ttacggctgg ttttcaatgg ttataaaaaa gccagttttg
     2041 tgctggctaa aaaacttgag caatgcacgt caggtgcaat tacgaaatct gacttaagac
     2101 cggatatcta tccgaaagat taacagaaca ccttcaattt ttaaccacag aacgatgagg
     2161 ctaaccgtgg gtaagtatca ctggaaagta gaaaaacagc ctgagtggta cgtgaaagct
     2221 gtcagaaaaa ctatcgcagc gttgccgggt ggttacgctg aagcagctga ctggctggat
     2281 gtaacagaga acgcattatt taaccgcctt cgtgccgatg gcgatcagat tttcccgctg
     2341 ggatgggcaa tggttttaca gcgcgcggct ggcactcact acattgcgga tgctgtcgca
     2401 cagtctgctg gtggggtgtt cgtatcgctt cctgaaattg aggaagtaga gaacgccgat
     2461 ataaaccagc gcctgctgga agtcatcgaa cagatcggga attactcaaa gcagattcgt
     2521 tcggcaatcg aagatggggt cgtggagcca cacgagcaga cagcaattaa tgatgagttg
     2581 tatctgtcaa tttcgaagct ccaggagcat gcagcactgg tctacaaaat cttttgcgct
     2641 ccagaaaaga gtgacgcccg cgagtgtgca gctccgggcg tcgtggcgtt ttgtgtctgt
     2701 ggagaaacta acgcatgaac agtttaacgg caaataaccg tttgtcgcaa cagctggtgg
     2761 tcagtgtcgc tgaacacctg ttgttacggc atgaatgcag attaccaaat cacctggctg
     2821 taagtaacca cagagaactt tacctgactg tggggggcga gttgtgcagg aacttaaccg
     2881 ctggtttcgt gacggaagag ggctttatgt ccatgttatt cgttgggagc cagaaacaca
     2941 gcgcgttatc tatcttcgca aagactaccc gcatgagtgc tttagtcctt tgtggaaatt
     3001 caggcgtgat tttgttgagt gtgaaggacc accagcacat tgattctgcc attccgggac
     3061 gttacactgt tcaggcacct tataaagcgg gtgccgggcg tggaaacccg gaattcacca
     3121 aagcgcacaa ccgcgctctt gcggtttttt tgtgtcatga gcagcattac gcccaaatta
     3181 tggtggggcg tgcagggcca acttcggttg ggccgggttc tttggtgacc ggtatttcca
     3241 cccctgtacg tctcaccacc aataaggtcg tggaaagcct tggtggtgag ttattaaaaa
     3301 tcaccaaaga ggctgccatc atggctacga tcccaaccct cactcaaccc gaaattgcca
     3361 tcgttgatgg tcaggctgtt acttcatccc tggctgttgc caacttcttc tccaaacgtc
     3421 atgacgatgt tctgaaaaag atccgcacgc ttgaatgctc cgcatcattc actgcccgca
     3481 atttttcggt gagtgattac accgattgca caggccgcaa actaccttgc tatcaaataa
     3541 cccgcgacgg ctttgcgttt cttgctatgg gtttcacggg taaacgtgct gcccagttca
     3601 aagaggcata catcaatgcc tttaaccaga tggagaaaca gctttcaaag ctcgctgtac
     3661 cgagtgacgt tgcacataac gccagcgttc tctattccta catttcatca attcatcagg
     3721 tctggttgca gcagctttat cccatgctgg aaaaagctga atcaccgctg gctgtaagtc
     3781 tgtatgaccg aattaacgat gcggcatttc ttgcccgtct tattcattcg tcgctgaact
     3841 cttcagaggt aagggggcgc aaatgatccg aaatattttc aaacggttta ccaatcaaac
     3901 tttccgttgt cctcgcccgg gtcagtggta caccacgcct gcagggcatg ttctacgtgt
     3961 tagcctggtt gaccgtgaat gtcagaaggt gatttgtgaa ccgctgggtc gtaattaccg
     4021 cgtcagtatg ccgctcatag cctttcgctc cggaaaaaac atgaagcatc tcggaggtgc
     4081 agcatgagta tggagctgat ggttaaagcg atgaaaattc gagtgggtaa tccattgcga
     4141 aaactggttc tgatcaagct ggctgataat gccagcgatc agggtgagtg ctggcccagc
     4201 taccagcata ttgctgacca gtgcgagatt agcaaacgtt ctgtgatgaa tcatattgcg
     4261 gccctttgtg agtccgggct ggtaaaaaaa gtcacccgga aaggtgaaaa aggtaactca
     4321 agtaatatct atctccttca tctggatggt gcaggagatt cactaggggg tagtgcaaat
     4381 aattcactat ctggtgcagc aaattcacca ggtagtgcag gagttgcacc agggggtagt
     4441 gcaggagatt cacccagaac cagtcactct tttgaaccag tcaaagaacc agtcaatgaa
     4501 ccaatagctg ttggtgcatc tgctgatgag tctgtgcgag ttcgttcaaa ccgaccggaa
     4561 tactctccgg agtttgagca ggcatggctg gcatacccca aacgtgctgg tggcaattca
     4621 aaatcagcag ccttcaaagc ctggaaagcc cgtttgaacg agggggtaaa ccccgaaacc
     4681 atgctggaag gtgtgaaacg ctacgcgggc tgggtatctg cgatgggcaa tagcggcaca
     4741 caatttgtga aacaggctgt cacgttcttt ggtccggatc gtcatttcga agaatcctgg
     4801 gaagttcctg cggtatctgc agccagacgc gatgacccgt acttcaaagc cagttacgac
     4861 aacgtggact acagccagat cccggcagga ttcagggggt gatcatgagt ctgttaaatg
     4921 acgttcagaa attcattgaa gcccatccgg ggtgtgcttc cggagacatt gcggatgctt
     4981 ttgcaggtta ctcacgacag cgcgttctgc agtcagcaag caagttacgt cagagtgggc
     5041 gtgtggctca ccgttgtgaa ggggatactc gcagacattt cccgcgccag actgaggttt
     5101 tgcaggaatc ggaaccgcaa ccagttcgtg aaaccagacc tgtgcgcaat ttctatgtcg
     5161 gcactaacga cccccgggtg attttgtgcc tgacccgcca ggctgaagaa ctggagtcca
     5221 ggggcttata ccgtcgtgct gcaacggtgt ggatggcggc attccgtgaa agccactccc
     5281 agccagaacg aaacaatttt ctggcgcgtc gtgagcggtg cttacggaaa agcagcaagc
     5341 gcgctgcatc gggtgaagag tggtatctgt cagggaatta cgtgggggct taatgagtaa
     5401 taaatattgc caggcgctgg tggaactgcg gaacaaacca gcccatgaac tgaaggaagt
     5461 gggcgatcag tggcgcacgc cggacaacat tttctgggga attaacaccc tgtttggccc
     5521 gtttgttctg gatctgttta ctgacggtga taacgccaaa tgtgccgcgt attacacggc
     5581 ggaagataac gcgctggcgc atgactggtc agaacgtctt gcggagctta aaggtgctgc
     5641 ctttggtaat cccccataca gccgcgccag tcagcatgag gggcaataca tcaccggcat
     5701 gcgttacatc atgaaacatg ccagttccat gcgtgataag ggcgggcgct atgttttcct
     5761 gatcaaagct gccaccagcg aagtgtggtg gccggaagat gcggaccata ttgcttttat
     5821 tcgcgggcgt attggttttg aactgcctgc ctggtttatc ccgaaggacg agaagcaggt
     5881 gccgacagga gcttttttcg ctggtgctat tgctgttttt gacaagacct ggaagggacc
     5941 ggcaatcagc tacatcgggc gcgatgaact tgaggcatgt ggtgaggcgt ttctggcgca
     6001 ggttcgccag caggcggaaa aactggtcag ggagatggcg gcatgacgac gttaactcaa
     6061 tgccagcagc aggtgctgga tatgctgatt tcttatcaga aagaacgtgg cttcccgcca
     6121 accaatcagg aggtggcaac catgctggga taccgttcgg tgaatgcagc ggtggagcat
     6181 cttcgcgcac tggagaaaaa aggcgtcatc acgataaagc gtggtgtggc ccgggggatc
     6241 acgcttcata ccgcggtgaa ggacgacgac agcgaggcgg tcgggattat ccgctcactg
     6301 cttgccggtg aggaaaacgc aaggctgcgt gcaactcact ggttacatga gagaggcctg
     6361 aaagtatgaa gctgatcctg cctttcccgc ccagcgtgaa cacgtactgg cgacacccca
     6421 acaaaggggc atttgctggt aagagcctga taagcgaggc ggggcgaaaa tttcagagcg
     6481 cggcgtgcgc agcaatagtt gagcagttac gtcgtctgcc gaaaccaacg tcggcacctg
     6541 cttcagtgga gatcgtgttg tttcctccgg ataaccggat ccgcgatctg gacaactata
     6601 acaaggcgct gtttgacgcc ctgacccacg cgggtgtgtg ggaagacgac agccaggtga
     6661 aaagaatgct ggtggagtgg ggaccggtta tcccgaaagg gaaggtcgag atcactatca
     6721 gtaagtatga gaaaccggcg ggtgcagccg cctgattaag aggagaaacg aagtatgaat
     6781 aatctgatgg tcattgatgg tattgaagtt cgtcgtgatg cttatgggcg ttacagcctg
     6841 aacgatctgc acagggcagc cgggggagaa caaaaaaaac gcccgaaata ctggctctcc
     6901 aataagcaaa cctgtgaatt gattgaacaa cttttcaccg agggtggaat tccgcctctg
     6961 gaaaagaatc aaccagttag cgttattaat ggcggaaata accaggggac gtatgtctgc
     7021 aaagaactgg tgtatgccta tgcaatgtgg atcagcccgt cattccatct gaaggtgatc
     7081 cgtactttcg atatggtaac cagcgcatcg gaaaaattat ccgggcaggc tgctgacaag
     7141 atgcaggctg gcgtgattct gctggacttt atgcgccggg agttaaacct gtctaactct
     7201 tcagtgcttg gtgcctgtca gaaactccag gaggctgttg gcttaccgaa tctggcaccg
     7261 cgctatgcca ttgatgctcc tgctgacgcg cctgatggct caagccgtcc cacgctgtca
     7321 ctgagtgcac tgctgaagca gtatggtatc cgcctgacag ctaatcaggc atatcaccag
     7381 atggtgaagc tggggatcgt cgagcagcgc gaacgataca gccgtaccgc gattaacaac
     7441 atcaaaaaat tctggtcgct gacagcgaaa ggctgcatgt tcggcaagaa catcaccagt
     7501 cccgcaaatc cgcgcgagac gcagccgcat ttcttcgaat cccgattccc tgagctgtta
     7561 aagctgctcg ataccgttca ttgaggtgac cgtgagagca ctactgaccc ctgaaattgc
     7621 cccgcgtatg gggatcgtat tgttcaggcc
//
LOCUS       DAZGLJ010000207         7629 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000207 DAZGLJ010000000
VERSION     DAZGLJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.207"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            94..1314
                     /gene="argD"
                     /locus_tag="ACHK49_003990"
                     /gene_synonym="dapC"
     CDS             94..1314
                     /gene="argD"
                     /locus_tag="ACHK49_003990"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO9264538.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(1381..3471)
                     /locus_tag="ACHK49_003991"
     CDS             complement(1381..3471)
                     /locus_tag="ACHK49_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO9264539.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(3522..4154)
                     /gene="crp"
                     /locus_tag="ACHK49_003992"
     CDS             complement(3522..4154)
                     /gene="crp"
                     /locus_tag="ACHK49_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO9264540.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            4456..4860
                     /locus_tag="ACHK49_003993"
     CDS             4456..4860
                     /locus_tag="ACHK49_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO9264541.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            complement(4915..5784)
                     /locus_tag="ACHK49_003994"
     CDS             complement(4915..5784)
                     /locus_tag="ACHK49_003994"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO9264542.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(5838..6056)
                     /locus_tag="ACHK49_003995"
     CDS             complement(5838..6056)
                     /locus_tag="ACHK49_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO9264543.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            complement(6050..7072)
                     /locus_tag="ACHK49_003996"
     CDS             complement(6050..7072)
                     /locus_tag="ACHK49_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO9264544.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            complement(7072..>7629)
                     /locus_tag="ACHK49_003997"
     CDS             complement(7072..>7629)
                     /locus_tag="ACHK49_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9264545.1"
                     /translation="LLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDD
                     LYLVHDRKVEPFDGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELR
                     AQTQPLRKEIARLEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSG
                     LEECEMAWLEAQEQLEQMLLEGQSN"
BASE COUNT         1732 a         1963 c         2056 g         1878 t
ORIGIN      
        1 atcgctgatt tctgattgcc attcaatgat tttttatgca tattttgtga ttataatttc
       61 acatttattt atgcgtaaca gggtgatcat gagatggcaa ttgaacaaac agcaattaca
      121 cgcgcgactt tcgatgaagt gatcctgccg atttatgctc cggcagagtt cattccggta
      181 aaaggtcagg gcagccgaat ctgggatcag caaggcaagg agtatgtcga tttcgcgggt
      241 ggcattgcag ttacggcgtt gggccattgc catcctgcgc tggtgaacgc gttaaaaacc
      301 cagggcgaaa ctctgtggca tatcagtaac gttttcacca atgaaccggc gctgcgtctt
      361 gggcgcaaac tgattgaggc gacgtttgcc gaacgcgtgg tgtttatgaa ctccggcacg
      421 gaagctaacg aaaccgcctt taaactggca cgccattacg cctgtgtgcg tcatagtccg
      481 ttcaaaacca aaattattgc cttccataac gcttttcatg gtcgctcgct gtttaccgtc
      541 tcggtgggcg ggcagccaaa atattccgac ggctttgggc caaaaccggc agacatcatc
      601 cacgttcctt ttaacgatct ccacgcagtg aaagcggtga tggatgatca cacctgtgcg
      661 gtggtggttg agccgatcca gggcgagggc ggtgtgacgg cagcgacgcc agagtttttg
      721 cagggcttgc gcgagctgtg cgatcaacat caggcattat tggtgtttga tgaagtacag
      781 tgcgggatgg ggcggaccgg cgatttgttt gcttacatgc actacggcgt gacgccggat
      841 attctgacct ctgcgaaagc gttaggcggc ggcttcccga ttagcgccat gctgaccacg
      901 gcagaaattg cttctgcgtt tcatcctggt tctcacggtt ccacctacgg cggtaatcct
      961 ctggcctgtg cagtagcggg ggcggcgttt gatatcatca atacccctga agtgctggaa
     1021 ggcattcagg cgaaacgcca gcgttttgtt gaccatctgc agaagatcga tcagcagtac
     1081 gatgtgttta gcgatattcg cggtatgggg ctgttgattg gcgcagagct gaaaccacag
     1141 tacaaaggtc gggcgcgtga tttcctgtat gcgggcgcag aggctggcgt aatggtgctg
     1201 aatgccggac cggatgtgat gcgttttgca ccgtcgctgg tggtggaaga tgcggatatc
     1261 gatgaaggga tgcaacgttt cgcccacgcg gtggcgaagg tggttggggc gtaaatacga
     1321 agcatcaggc aaaagatgtc ggatgcggcg tgaatgcctt atccgatggt ttagcctgca
     1381 ttacgccttc gaatcccgca acttgcgact cagccaaatc ccgtgatgcg gtcgctgacg
     1441 ccatgccatc gacgaaatgg tgtgcatggt gttcagatga ccaataaccc gctgtaaatg
     1501 ctgctccagc gtacctgccg cgccttcgtg cggctgcatc tccggcgcat ccatgatatt
     1561 ggcatcgcca gaactacccg gttcgtcata ctccagtcgc tgctgacaac gctgaatggc
     1621 gatttcacaa gactgtaaat actcttgtgc cagttcaggt ggcaatgccc ggtgttcccg
     1681 cgccagcgtg gtcatggcat taatatgctc aacaataaac tggctgtgcg ttacccacag
     1741 tttcatatct gccagataat ggctgttaaa cgccggttcc tgcatcgcct gattcaatga
     1801 gttatacaga gtgttatgtg cctgatttac ccgcattcgc tgccaggcca gtggcgtagg
     1861 ttgcggatcc tcgctaagaa tcaagcgaat cgcttcctga taggcttcta aagcgtcatg
     1921 ggcgttttta cgcaataacc cgctctgcca ctgcggccac agccagacag taccgccgaa
     1981 agcaattaaa caaccaataa tggtatcgat aagacgcgga aggatgtatt gctcgccgtt
     2041 caaccacaat agttgcaggg tatacactgc ggtaatagta aaaccgaccg tcgcccagcc
     2101 gtagtttttg cgcaatatca ggtagctggc gagggtggta atcagcatca acgtcagggt
     2161 gtaaccttcg ggaattttaa agtgcagcgc cacgcccgca atgattaacc cgaccacggt
     2221 tcccacggag cgattcacaa tcctcagacg ggttgcgcca tagccatttt gtgtcaccag
     2281 caataccgtc atcaggatcc agtacgactt cggcagatgc agcgcggtgc ccatcaggct
     2341 ggcaacgctt aacatcacac tgagtcgtcc ggcattgcgt agcgccggag actttagtga
     2401 cagataactt ttcagcgccg gaagtaatgg cattcgccgc tgtttatcgg ccagtaagtc
     2461 acgtgcatag agcggttttt gggtgcgcag cacgcgggcg atgcggctga aatgccagta
     2521 gcagaattgc ccaaccggat tatccggatg ctggcgggcg attttttcca gtgcgccaat
     2581 ttgcttttcc atcgtaaaac gcgttggcag gcgatggtaa agaatgtcat cagccagcac
     2641 gcgcaggcga gcggcgacgg tttgcgcatt ccagcggata acttcttccg catggctacg
     2701 ctcgaccagc ttttgcacct cttccggctg atgcaaactg accgaaatat gttcctgtaa
     2761 atccagcgcc tcctggaaaa tacgcagcat ccgcttgtag tcagtattat tttgcgcgga
     2821 aagcatatgc atttgctgat agcactgggt aattagatcg accgcttttt gctggcgcac
     2881 cagcagcggc ggcagcgctt tttcagggtc ggtgtgctgg gtaagcaggc tgtatttggc
     2941 ttcacaataa tctgccagtt cacggtacag cagacttagt gactcgcgca gcggttgttc
     3001 gcgccagatc cagaaccaaa accagttaaa caatccgtac cagagagtgc ccaacgcata
     3061 gatgagcaac ggttcccaga ccggcatgta tcccgccaga ctgagggtaa aaatggctgc
     3121 cagcagcgat gcgggaagca attttgcgtg caatggcccc aactcagcag tgacgccaag
     3181 taccagcgtt aatccggtca gcaaaaaggg caggggaaca tcttttgcca gtagcagctg
     3241 tgtcagtaag ctacaggtgg caaacagcga cgcaccaatg attaagcgtt tgaaaaaacg
     3301 tttatgaggc gtatcaaggc ccgcaatatt gcaacaggca ggaacgagag agaagagcag
     3361 accgaatcgt aattcgccaa gcattaaccc aacggccacg ggcaaacata gcaccagcgt
     3421 ttgtcgaagt gcatagttga tatcggggtg ataaatcagt ctgcgccaca tcgggggaaa
     3481 caaaatggcg cgctaccagg taacgcgcca ctccgacggg attaacgagt gccgtaaacg
     3541 acgatggttt taccgtgtgc ggagatcagg ttctgatctt ccagcatctt cagaatgcgt
     3601 cccacggttt cacgagaaca gccgacaatc tgaccaattt cctgacgggt aattttgatt
     3661 tgcataccgt ccgggtgagt catagcgtct ggttgttttg ccagattcag cagagtctgt
     3721 gcaatgcggc ccgtcacgtc gaggaacgcc aggttgccca ctttctctga agtgacttgc
     3781 agacgacgcg ccatctgtgc agacaaacgc atcagaatgt ccgggtttac ctgaatcaat
     3841 tggcgaaatt ttttgtacga aatttcagcc acttcacagg cggttttcgc acgtacccat
     3901 gcgctacgtt cctggccctc ttcaaacagg cccagttcgc caataaaatc accctgattc
     3961 agataggaga ggatcatttc tttaccctct tcgtctttga tcagcactgc cacagagcct
     4021 ttaacgatgt agtacagcgt ttccgctttt tcaccctggt gaataagcgt gctcttggat
     4081 gggtacttat gaatgtggca atgagacaag aaccattcga gagtcgggtc tgtttgcggt
     4141 ttgccaagca ccatgcgcgg ttatcctctg ttataagctt tctccagagc cagataacgc
     4201 cgctgtctct ggattgccga aatatgcttc ccgctacctg ggaaggggct atcaactgta
     4261 ctgcacggta atgtgacgtc ctttgcatac atgcagtaca tcaatgtatt actgtagcat
     4321 cctgactgtt ttagcatagc tttcgctttg tgtctcctgg tgtctcgctt cagcatgacc
     4381 caggtcgcct tccgttgcgc gatttggtta gtacgcgtac tctgtcagga aaattgacgc
     4441 agtggagtag caaaaatgca agcgcgagtg aagtgggtcg aagggttaac ttttctgggc
     4501 gaatccgcct ctggtcatca gattttaatg gacggcaact caggcgataa agcaccaagt
     4561 ccgatggaaa tggtgttgat ggcggcgggt ggttgcagtg ccatcgatgt ggtttcgatc
     4621 ctgcaaaaag ggcgtcagga tgtggtcgat tgtgaagtaa aattgacctc tgaacgccgc
     4681 gaagaggcac cacgcctgtt tacgcacatt aatctgcatt ttatcgtcac cggtcgcgac
     4741 ctgaaagacg cagcggttgc gcgtgcggtt gatctctctg ccgagaaata ttgttcagtg
     4801 gcgttgatgc tggaaaaagc ggtgaatatt actcactcgt atgaagtggt tgccgcgtga
     4861 cttaactatc ccgttgtgaa gccggatagt attttatccg gcgtaatagc agagttactc
     4921 gattttcttt ccttccatca atcgttgcac cagcggcagc ataattaatt ccattgccag
     4981 acccattttg ccgcccggta ccactaacgt attgatgtgg gaaatgaatg agccttgcaa
     5041 catcgccagc agccagggga aatcgatccc ttccagatta cgaaaatgga tcaccacaaa
     5101 gctttcatcg agcgacggga taccttttgc cgcgaacggg tttgaagtgt cgacggtggg
     5161 aacgcgctgg aagttaagat gggtgcggga aaactgcggt gtgatgtagt tgatatagtc
     5221 ttccattgaa cgcactactg agtccatcac tgcttctcgt gagtgcccgc gctcgctggt
     5281 gtcgcggatc agtttttgaa tccactcaag gttaacgata ggcaccacgc cgaccagtaa
     5341 gtccacatgc tgcgcaacgt tatgctgtgg cgtgactacg ccgccgtgta aaccttcata
     5401 aaacagtaca tcagtgggtt ccggtaaagg ttgccagggg gtgaatgtcc ccggtacctg
     5461 attccacggt acggcttcgt cgtaggtatg cagatatttg cgagatttcc ctttgccgct
     5521 ctgaccgtat tcaatgaagg tttgttccag caggccgaag tcgttagcct cggggccgaa
     5581 gtagctgata tgccgcccgg cgtcgcgcgc tttgcggatc gccatgtcca tttccgggcg
     5641 ggtgtaacgg tgaaaactgt cgccttccac ctcagctgca tgcagattta actgcgcgaa
     5701 tattttacga aacgcgaggc tggtggtggt ggtccccgcg ccgctggatc ctgttaccgc
     5761 aatgaccgga tgtttggcag acatagtaac tccatgactt agctatcatt attatctcgt
     5821 ccgccgacgg ttgcaggtta ttcgcgaaac tgactgcgcg gcataatatt gaccgtttcg
     5881 tgcagctccg accataccag caccgcttct ccgcactgta gctggcgttt gacgtcggcg
     5941 actttctgtt caagtgtacg ctcatgttca ccataatcgg tgccttcacg taacacaaag
     6001 ctttcaatta aattttccag cgtttcgggg gagaggtctt gccacggaat caacatgatt
     6061 tcgcctccag atacgttgtt aaccagtcag gaatgcgtga ctccagccac atttgcggat
     6121 gaagtaatgt accgccaata aagccaacat gaccgccatg ttcagtcagt tgatactcca
     6181 cctgcggggg gagactttcc ggtttcggga tcacctgatg atccataaac ggatcgtctt
     6241 tggcgtgaat aatcagcgtc ggtttggcga tccggttcag catcggcatg gcgctacact
     6301 gacgataata gtcgatagcg tcggcgtagc cgtgaattct ggcggtgatg agatcgtcaa
     6361 attcacggat gcgacgtacc gattttaact gcgcgagatt aatcggcagc gttccggggt
     6421 aggctgccag cttgcgcgcg gcattggctt ttaacaggtt cagcaagtaa cgctgataaa
     6481 cgcgggaaaa gcccttttcc atatgatagc tacaggcttc cagcataaac ggcgcagaga
     6541 caatcaccgc cgcatcaacc gggagatcat tgccttcctt tgccagcaaa caggccagca
     6601 tattaccgcc gagcgaatag ccgacggcag ccgttggcgc atgaccaaat tcgcgttgca
     6661 gccagcgtaa aaaccaactg gcgtcttcgg tttcgcccga atggtaaatg cggtgcatac
     6721 ggtttggttc accgctgcat ccgcgaaaat gcatcaccac gcccagccag ccgcgctttt
     6781 gcgccgcctc aaccagaccg tgggcgtaag ggctgttgag actgccttcc agcccgtgaa
     6841 acaccaccag acgaggttta tgttgcgcct gtgcagggtt ttcactccac gcaagatcga
     6901 caaaatcgcc gtcgggcaac tccagccgct gccagtacgg ggtgaatttc acctgacgac
     6961 gaaacagacg cggcagcatg gtttgcagat gacaattgct aaagccgcgc atggggatga
     7021 attcagcact gctgctgaat tcattggcat cggtcgtcgt tatctgcgcc atcagttgct
     7081 ttggccttcc agcagcatct gctcaagctg ctcctgggct tccagccatg ccatttcgca
     7141 ctcttccagg ccggatttgg cgctggcttg ctgttgcagg caggcggtca actccgcttt
     7201 acggctctgg tcatacagtt cgctgtcgcc gagtttctct tccgcctgcg ccagttgcgc
     7261 gttcagcttc tccatctctt tttccagacg ggcaatctct ttacgcagtg gctgggtttg
     7321 cgcacgcagc tcagcttccc gacgcttctg atctttacgt gcctgggcgc tgttcgcatt
     7381 ctcttttggc gcttcgtcgg tctggttttc ctgcttttgt acgtcgctca accactgttg
     7441 ataatcttcc agatcgccgt cgaacggttc gactttacga tcgtgaacca ggtagagatc
     7501 gtcagtggtg gaacgcagca aatgacggtc gtgcgaaacg acaaccagcg cgccttcaaa
     7561 ctcgattaat gcttcggtga gtgcctgacg catgtcgagg tcaaggtggt tagtcggttc
     7621 gtcgagcag
//
LOCUS       DAZGLJ010000208         7595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000208 DAZGLJ010000000
VERSION     DAZGLJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.208"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            289..1560
                     /locus_tag="ACHK49_003998"
     CDS             289..1560
                     /locus_tag="ACHK49_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO9264546.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(1590..2594)
                     /gene="dppF"
                     /locus_tag="ACHK49_003999"
     CDS             complement(1590..2594)
                     /gene="dppF"
                     /locus_tag="ACHK49_003999"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO9264547.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSFSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(2591..3574)
                     /gene="dppD"
                     /locus_tag="ACHK49_004000"
     CDS             complement(2591..3574)
                     /gene="dppD"
                     /locus_tag="ACHK49_004000"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO9264548.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDIYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(3585..4487)
                     /gene="dppC"
                     /locus_tag="ACHK49_004001"
     CDS             complement(3585..4487)
                     /gene="dppC"
                     /locus_tag="ACHK49_004001"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO9264549.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(4497..5516)
                     /gene="dppB"
                     /locus_tag="ACHK49_004002"
     CDS             complement(4497..5516)
                     /gene="dppB"
                     /locus_tag="ACHK49_004002"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO9264550.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRLQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(5667..7274)
                     /gene="dppA"
                     /locus_tag="ACHK49_004003"
     CDS             complement(5667..7274)
                     /gene="dppA"
                     /locus_tag="ACHK49_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO9264551.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
BASE COUNT         1712 a         2088 c         1947 g         1848 t
ORIGIN      
        1 cgctgatttt acgattgctt tttactgtca gcacccggca atcttcctca ttgcgcgatg
       61 ccttccagct taatttcttt atttgcaata gttgcagttc atatccgggc tgactccaga
      121 ctggtagtga aatgcgcaat tttgtagaat tgcgcctcct cgcgacctcc ccagtgaatc
      181 tgttgcctca ttttttacat actgcgtatt cgacttctcc acctgttgcg caagagaaac
      241 tgggtttatt catttttgcg agaccgactt ctttctggac aggactttat gcagcacaac
      301 acactatcga aacacaatca gaaattgccg tttacacgct acgacttcgg ctgggtttta
      361 ttatgcatag gcatggcgat tggtgccgga accgtgctga tgccagtaca aattggcttg
      421 aagggaattt gggtatttat taccgcagcg atcattgctt atcctgccac ctgggtagtg
      481 caggacattt atttaaaaac cctttctgaa agcgattcct gtaatgacta caccgatatt
      541 atcagtcatt acctggggaa gaactgggga attttcctcg gggttatcta ctttttgatg
      601 attatccacg ggatttttat ctactctctc tccgtggttt tcgacagcgc ctcgtacctg
      661 aaaaccttcg gtttaaccga tgccgatctt tcacaatctc tactttataa agtcgctatt
      721 ttcgccgtac tggtggcgat tgcgtctggt ggtgaacgat tactgtttaa gatttccggg
      781 ccaatggtgg tggtcaaagt agggattatt gtcgtgttcg gttttgcgat gatcccgcac
      841 tggaatttcg ccaatataac cgccttcccg caagcctccg tctttttccg cgatgtcctg
      901 cttacaattc cattttgctt cttttctgcg gtatttattc aggtacttaa cccaatgaat
      961 attgcctatc gtaaacggga agcagataaa gtactggcaa cccggctcgc gctgcgtacc
     1021 caccgaatta gttatatcac gctcatcgcg gtgatcctgt tttttgcctt ttcgtttacc
     1081 ttctcaatta gccacgaaga agccgtttct gcctttgaac aaaatatctc ggcactggcg
     1141 ctggccgcgc aggtgatccc tggacatatc attcatatca cctctacggt gctgaatatc
     1201 tttgccgtac tgaccgcatt ctttggcatt tatctcggtt tccacgaggc tattaaaggc
     1261 attattctca atctgttaag ccgaattatt gataccaaga aaattaactc acgcatgctg
     1321 actctggcga tctgcgcttt tatcgtcatt acattgacga tttgggtttc gtttcgtgta
     1381 tcggtgctgg tgttctttca gttgggaagc ccgttatatg gcattgtgtc gtgcctcatt
     1441 ccgtttttcc tgatctataa agtcgcacaa ctggaaaaac ttcgcggatt taaagcctgg
     1501 ctaattctgc tttacggcat tttgctatgc ttgtcgccac tgttgaagct gattgagtaa
     1561 accggagcgc atggccccgg ttttgtgagt taacgctgcg gattttcatc ctgatcaaca
     1621 gcaaaacaag cgaccagttg accgccgtag tcttttagct gcggctgcaa ctgggtgcag
     1681 gggccgaagc gccgacgaca gcgggcgttg aaggcgcaac ccggcggagg attcagcggg
     1741 cttggcagtt caccggtgag tttgatgcgt tcacgacgat cgtccgggtt caggcgtggc
     1801 gtcgcggaga gcagcgcctg ggtgtacgga tggcgcgggt tattgaagat ttggtccttc
     1861 gttcctttct ccacgcagcg gcccaggtac atcaccatca cttcatcagc aatgtgctcc
     1921 accaccgaca ggtcgtggga gataaagaca taagaaagcc ccaactcctg ctgcaaatcc
     1981 atcatcagat tcagcacctg cgcacgcact gagacgtcca gcgcagaaac cggttcatcg
     2041 gcaatcacca cgtccgggtc gagcatcaga ccacgggcaa tagcgatacg ctgacgttgt
     2101 ccgccggaga acatatgcgg atagcggtca tagtgctcgg ttttcaggcc gactttcgcc
     2161 atcatcgaca gggctttttc ccgacgctgt tctttgctga agctggtgtt aataagcagc
     2221 ggctcttcga gaatttgccc aactttttta cgcggattca gcgaaccgta agggttctgg
     2281 aagacaatct ggattttctg ccgacgcagc ttctgcgcct gcggatcgtg cttgagcaga
     2341 tcctgcccct ggtaatacag ctcaccaccg gtgggcgttt cgatcatcgt cagcaaccga
     2401 ccgagggtcg atttaccgca gccagattcg cccactactg ccagcgtttt gccacgttca
     2461 aggttaaacg aaacgccgtc cagcgccttc accagacgtt ccggcgcgaa catgcctttc
     2521 ttcaccggat aatgtttttt caggtcgata gcctgcaaca gcggttgttg cgaggtggcc
     2581 tcttgcgtac tcatagtgtc ggcctcccgg catcatcaag tgggtaatgg catttggact
     2641 gacgcccatc agcgagcata ttcagcgccg gttcttcagc gcgacatctg tcagtggcat
     2701 aggggcagcg cgggttaagc aggcagccgt tcgggcggtc gtacttaccg ggaacgacac
     2761 ctggcaacga cgccagacgt tctttgtcct gagcaaattc tggcagcgca cgcagcaatg
     2821 cctgagtata cgggtgacgc ggcgcatgga agatggcgtg cgcatcaccg gtttccacca
     2881 cctggcctgc atacatcacg atgattttat gtgccgcttc cgccaccagc gccaggtcat
     2941 gggtaattaa caccagcgcc atgttctctt tctgctgtag ctccagcagt agttcgatga
     3001 tttgcgcctg aatggtcacg tccagcgcgg tggtcggttc atcggcaatc agcagttttg
     3061 gccgacaggc aatcgccatg gcgatcatca cgcgctggct catgccgccg gaaagctgat
     3121 gcgggtaaat atccagacgc gatgccggat cgggaatacc gacctgattc agcaggtcaa
     3181 tcgcccgctg acggcgagtg ctcttattac cgccctgatg caccttaatc gcttccatga
     3241 tctggaaacc cacggtgtag cacgggttaa ggctggtcat cgggtcctgg aagatcatcg
     3301 ccacttcggc acccaccagg ttgcggcgct ctttttctga tatacgctgc aaatcctgac
     3361 cgttaaacac cagcttttcc gccattacgc ggcccggata atcaatcagc cccataatcg
     3421 ccagtgaact gaccgactta ccggaaccag actcacccac aatcccgacc acttcgccct
     3481 gttttacgct gtagctgatg cggtctacgg cgcggaacgg tgcgctttcg tcgccgaaat
     3541 gcaccgataa tttatctaca tttaataacg ccatctcgaa cctcttactg cttcagtttg
     3601 ggatcgagcg cgtcacgcag accgtcaccc atcaggttaa atgccagcac cgtcagcagg
     3661 atcgccagac ccgggaaggt cacgacccac caggcgcttt gcgcgaactg caacacgtcg
     3721 gagagcatgg tgccccactc tggtgttggc ggctgcgctc ccatgccgag gaagccaagg
     3781 gcggccatat cgagaatggc gttagagaaa ccgagcgacg cctgaacaat cagcggcgca
     3841 aggcagttcg ggaagatgtt aataaacatc tgacgcatcg ccccggcacc cgccacgcga
     3901 gacgcggtga cgtaatcgcg gttaacttcc accagcacgg cggcgcgggt taagcgcaca
     3961 tagtgcggca aggcaacgaa ggtcagtgcc agcgcggcgt taccaatcga cgggccgaag
     4021 attgccacca gcaccagcgc cagcagcaga cttggcagcg ccagcatgat atcgaccacg
     4081 cgcataatga tgttatcgac caggccgcca aagtaaccgg cgatcaggcc gagaataacg
     4141 cccataatca gcgataacac aactaccaga cagccaacca gcagcgacag gcgcgcaccg
     4201 tacatcaggc gcgacagcac atcacggcct acgtcatcgg tgcccagcaa gtgcgccatg
     4261 ctgccgcctt cctgccaggc tggcggggcg agcagtgcat cgcggaactg ttccgccggg
     4321 ttatagggtg caatccagtt ggcaaagatc gcgatgaaca gcacgatgac gacgtaaacc
     4381 agcccgacga ccgcgccttt gttgcgttta aaatagtgcc agaactcctg taacggggtc
     4441 atcggcaccg gtgcgctaat cactttattt tcagtaacct gtgacatgat gtccccttac
     4501 ttcttatgac gaatacgcgg gttcaccacg ccgtacagca gatcgaccag caagttgacg
     4561 aggataatca tcgtcgccac cagcaatacg ccgccctgca ctaccggata gtcgcggcgt
     4621 tgcagcgcgt caatcaacca gcgccccaga ccgggccacg agaagatggt ttcggtcaga
     4681 atcgcccccg ccagcaatgt tcccacctgc aggccgataa cggtcactac cggcagcatc
     4741 gcgttacgca gcgcatggac gataatcacc cgcatgcggg ttagcccttt ggcgcgcgcg
     4801 gtgcggatgt aatcctcgcc cagcacttcc agcatcgagg agcgtgtcat acgcacaatg
     4861 accgccagcg gaatagtacc cagcacaatg gcaggcaaga tcatatgggc gacggcatcg
     4921 ataaagttgc cgtcttcacc ccagatggcg gtgtcgatta gcataaaacc ggttaacgga
     4981 ttggagtcat cgaggaacac catatcgctc acgcgaccgg agacgggcgt caggttccag
     5041 tgcaccgaaa ccagcatgat cagcatcatg ccccaccaga agatcggcat cgagtagccg
     5101 gtcagcgcca ggccaaccgc tgtgtgatcg aaaatggaac cgcgtttaac cgcagccagc
     5161 acgccgaccg gaataccgac cgcagtcgca aaaatcatcg cgcagacgcc aagttccagc
     5221 gtggcctgga ggcgcggcac gaactcttcc caaaccggga tgcggctttt cattgaaatg
     5281 cctagatcgc catgcataac gccccaaatg taatggagat actgctgcca catcggttta
     5341 tctaagccga gttcagccag cagctgcgcg tgacgctctg gggagatccc acgttcgccc
     5401 gccatgatca tcaccggatc gcccgggatc atgtggacaa aggcaaatgt gagaagggta
     5461 ataccgataa acgtggggat gacgagtccc aaacgtcgga gaataaactg caacataacc
     5521 cggattctct atagtgaccc gcagcctgga actgcgtgtc tgtattgctc acaaatcaat
     5581 tcccgcctag gtcgggctgg gaatgaagta ttgctgccgg atggcgctgc acgcagaggc
     5641 acagcgccac cgggtatggc ttttaattat tcgatagaga cgttttcgaa gtgatgtttg
     5701 cctaatggat caaccacata gcctttaact tctttgcgta ccggttcaaa cacggtggag
     5761 tgagcgatga tcagtgccgg agcctgatcg tgcatcacca cctgcgcttg cttgtacagt
     5821 tcaacgcgtt tgttatggtc gtcggtagca cgcgccggtt gaatcagatc ttcaaacggt
     5881 ttgtagcacc attttgagta gttggagcct tgttcagagg cggcgcagct gaacagggtg
     5941 gcgaagaagt tatccggatc cccgttatcg ccagtccagc ccatcattac cgtctggtgc
     6001 tcgccatctt tcgcacgctt gaggtactca ccccattcgt aggtgacaat tttggcctgc
     6061 acgccgactt tcgcccagtc agcctgaatc atctccgcca tgcggcgagc gttcgggtta
     6121 tatgggcgct gtaccggcat cgcccacagg tcgatggaga aacctttttc cagacccgct
     6181 tctttcagca aggctttcgc tttttcagga tcgtaggtgt aatcctgaac gtcgtcgtta
     6241 tagccccaca tggttggcgg gatcaggttt ttcgctgata cacccgcgcc ctgataaacc
     6301 gctttgatga tcgcgtcttt gttcaccgcg taggtcagtg cctggcgaac tttcacgtca
     6361 tccagcggtt ttttctgcac gttataggag aggtagccga cgttcagccc cggcatttcc
     6421 atcaggttga tggatttatc ctgcttcatg cgagcgatgt ctgccgggtt cgggtacggc
     6481 atcacctggc attcattctt ctgcaatttc gcataacgca cggaagcgtc aggagtaata
     6541 gagaagacca gcgtatcgat ctgcggtttg gtgccccagt agccatcaaa cgctttgtag
     6601 cggatacggg agtctttttg gtactgctgt aactggaacg gaccggtgcc gattgggttg
     6661 aggtccagtt tttccggtgt accggctttc atcatcgcgt cagcatattc ttttgacaga
     6721 atagaggcga agtccattgc caggtcagcg aggaacggcg cttccgggcg agtcagcaca
     6781 aactgaacgg tgttgtcgtc cacctttttc acttcgctga tcagctccgg caagcccatg
     6841 ccttcgaagt attcgtagct gccgccagaa actttatggt acgggttttg cgcgtttttc
     6901 tgacgatcaa acgagaacac cacgtcatcg gcgttcagtt cacgcgtcgg tttgaattct
     6961 ttattgtcgt gccacttcac acctttacgc agatggaagg tataggtttt accgtcttcg
     7021 ctgacttccc acttttcagc gaggcccggg atcacttcag tggtgccgat tttaaattca
     7081 accaggcggt tataaagcgg tacggaagag gcgtcatagg tggtgccgga ggtaaacagc
     7141 tgcgggttaa acccttccgg agatccttct gagcaataaa ccagagtttt agcctgaaca
     7201 cttgctgcga cggtcatagc caccaggctg agaccaagct tcagcatccc tgactttttc
     7261 aaggaaatac gcattattct gctccaattg tgatgtttgt tgttttaacc ctttgcagtg
     7321 ggttgtcgct gcctgacctt ttttgttttt tgcccggtcg ggtcaacgtt atgaggtggg
     7381 gatgccgtac taattaacat cagtgtaaca gtgcggatcc tccataaaat gcccctcgtg
     7441 acctacaatc tgtcaacaga atgtgaaaac gtcaatacag ccaaccggga tttacaccaa
     7501 cggtgagaat ccacaaacaa agattaaaaa aacttcaaac agctatttgc agcagagaaa
     7561 tttgtgctac tccaacatga ccagaacaat cagct
//
LOCUS       DAZGLJ010000209         7594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000209 DAZGLJ010000000
VERSION     DAZGLJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.209"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(105..359)
                     /locus_tag="ACHK49_004004"
     CDS             complement(105..359)
                     /locus_tag="ACHK49_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO9264552.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            560..1294
                     /locus_tag="ACHK49_004005"
     CDS             560..1294
                     /locus_tag="ACHK49_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO9264553.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(1296..1907)
                     /gene="emtA"
                     /locus_tag="ACHK49_004006"
     CDS             complement(1296..1907)
                     /gene="emtA"
                     /locus_tag="ACHK49_004006"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO9264554.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            2007..2921
                     /gene="ldcA"
                     /locus_tag="ACHK49_004007"
     CDS             2007..2921
                     /gene="ldcA"
                     /locus_tag="ACHK49_004007"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO9264555.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            3016..4752
                     /locus_tag="ACHK49_004008"
     CDS             3016..4752
                     /locus_tag="ACHK49_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO9264556.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(5138..6208)
                     /gene="dadX"
                     /locus_tag="ACHK49_004009"
     CDS             complement(5138..6208)
                     /gene="dadX"
                     /locus_tag="ACHK49_004009"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO9264557.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(6218..7516)
                     /gene="dadA"
                     /locus_tag="ACHK49_004010"
     CDS             complement(6218..7516)
                     /gene="dadA"
                     /locus_tag="ACHK49_004010"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO9264558.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVHPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
BASE COUNT         1730 a         2053 c         1939 g         1872 t
ORIGIN      
        1 aagtgctata ttgatttgtt ccacccgtaa agcaggtgtc gagtatacgt tctgatgtga
       61 aagcggatag cggcatctgt atcgctatcc gccttatgaa aatcttattc tcttcgcagg
      121 aggcggaata cgcccagaac gagaatagct cccaccaccg ccaccaggaa gctgtgcaga
      181 ttaaaaccac tgatggagcc gccaatgcca aacatggtcg ccagccagcc gccgaccacc
      241 gcaccgacta tcccgagaat acaggtcagg aaaaatccac caccatcacg ccccggcatg
      301 attagcttgg cgataatgcc ggctatcagg ccaaaaataa tccaggcaat aattcccatg
      361 ttcaggccct cgtttcgatg aatcggacgc aagaaatgct tacgtcaacg ctctaagtat
      421 aggcaagggc gggatcgatg ctgggcgtat gtcggctgat ttaagaacaa catgtaaaaa
      481 atattcacgc atttgtatta agttttgtta actgtgaccg ataaaccaaa gacagtttgt
      541 cagtcaggag tttttccgcg tgagtcatta ccatgagcag ttcctgaaac aaaatccgtt
      601 agccgtcctg ggcgtgttac gcgatttgca caaagccgca attcctttgc gtctcagttg
      661 gaatggcggg cagctgatca gcaaaatact ggcaataacc ccggataaac tggtgctgga
      721 tttcggcagt caagccgaag acaacatcgc cgtgctaaag gcacagcaca ttaccattac
      781 cgccgaaact cagggtgcga aagtcgagtt tactgttgaa caactacagc agagtgaata
      841 cttgcagctt ccggcattta tcaccgtacc gcctcccacc ttatggtttg tacaacgacg
      901 ccgatatttc cgcatctccg ccccactcca tccgccttat ttttgccaga ccaaactggc
      961 ggataacagt acgttacgtt tccgcctgta tgatttgtcg ttaggcggca tgggcgcatt
     1021 actggaaaca gcaaagcctg ccgaattaca agaaggcatg cgcttcgctc agattgaagt
     1081 caacatgggg caatggggtg tttttcactt tgacgcccag ttaatctcca tcagcgagcg
     1141 caaagtgatt gatggcaaga atgaaaccat caccactccc cgtctgagct tccgttttct
     1201 taacgtcagc ccgacggtgg agcggcaatt acagcggatt attttctctc tcgagcgaga
     1261 agcccgggaa aaagcggaca aagtgcgcga ctgatttaca tcgcgtccag tgcctgctca
     1321 agtttgtaga tatagcgcgg agcctgcggc gcaggatgat ttcgcgctac gtgatcgagg
     1381 aactcgtcag catccaaatc gttgattttg ctgatcgcct ttttccgatc tgacgagaaa
     1441 gtccgtagca gcgcacctgc cccgttagcg tatgacacca ccagcgcata ttgcagtacc
     1501 ttcggatctt caatgcctgc cagcgggccg gtttccagaa tattcaggta agccgccccc
     1561 attgaaatat tacgctctgg atttttcagc tcgctggtcg tcggctcacc actccagccc
     1621 atacggcgat aaacatcacg tccggaggtt gaagctttta actgcatcaa accaatggca
     1681 ttcgatttac tcaccgcatt cggattacca cccgattcga tagcgataat cgccgtgatc
     1741 aattgtggat cgacgcccca ggctgcaccg gctttctggc ttattggcat ccactgcatc
     1801 gcacgttgca ccggaacttt cgcgttccac ggcgggttcg tatagtcatg ctttgatgaa
     1861 caacccgcta ataacacaat caaaaaggca aaccatctca atttcacttc ttctatcctt
     1921 atagcctgga atcgccgttg aggtgacaac ggcacaccta agcggcatga tatacatttc
     1981 gtttctgttg tcagcaagga attgccatgt ctctgtttca cttaattgcc ccatcgggtt
     2041 actgcattaa acagcacgcc gcgctgcgtg gtatccagcg ccttaccgac gaggggcatc
     2101 aggtcaataa cgtcgaggtt attgcccgtc gttgtgagcg ttttgcgggt acggaaacgg
     2161 agcgcctgga agatctcaat tcccttgcca gactgaccac ccccaacacc atcgtgctgg
     2221 ctgtacgcgg cggttacggt gccagtcgtt tactggcaga tattgactgg caggcactgg
     2281 ttgctcgcca acaatatgac ccgttgctca tatgcgggca tagcgatttt accgccattc
     2341 agtgcggtct tctggcgcaa ggcaatgtca tcacctttag cggccccatg ctggtagcaa
     2401 actttggcgc ggatgaactg aacgccttta ccgagcacca tttctggctg gcgttacgca
     2461 atgaaacctt caccattgaa tggcaagggg aaggcccgac atgtcagacc gaaggcacgt
     2521 tgtggggagg caatcttgcg atgctgattt cactgattgg tacaccgtgg atgccaaaaa
     2581 ttgagaacgg tattctggtg ctggaagata ttaacgagca ccctttccgc gtcgaacgta
     2641 tgctgttgca gctctatcat gcgggaattt tgccacgtca gaaggcgatt attctcggta
     2701 gctttagcgg cagcacgccc aatgattatg acgcaggtta caacctggag tcagtgtacg
     2761 cgtttttgcg ttcccgcctg tcgattccac tcattaccgg cctcgatttt ggtcatgaac
     2821 aacgcacggt tacgctgccg ttgggcgcac atgccattct gaataacacc cgggaaggca
     2881 ctcagttgac gattagcggt catcctgttc ttaaaatgta aaaaaatcac gcttcagggc
     2941 taagtaaaac gctgtctctg ccgctaatat ggtagggttt atataagaga cagcgtaatc
     3001 aggagtaacc gaccgttgga tgccacaaca ataattagcc ttttcatctt aggatccatc
     3061 cttgtcacca gcagtatatt acttagttca ttttcttccc gtcttggcat tcctattctg
     3121 gttatttttc tggcgatcgg catgctggca ggagtcgatg gcgtcggcgg tatcccgttt
     3181 gataattacc ccttcgccta catggtaagt aacctggcac tggcgattat tttgctcgac
     3241 ggcgggatgc gcactcaggc cagttccttt cgtgtggcgt taggaccggc actgtcgctg
     3301 gcgacgctgg gcgtgcttat cacctctggt ttaaccggca tgatggcggc gtggctgttt
     3361 aatcttgatt tgattgaagg cttattaatc ggcgctatcg tcggctctac cgatgctgca
     3421 gcggtctttt ctctgctggg tggtaagggg cttaacgaac gtgttggctc gacgctggaa
     3481 attgaatccg gcagtaatga tccaatggcg gtctttctga cgattaccct gattgcgatg
     3541 atccagcaac atgaaagcag tgtcagttgg atgttcgtgg tcgatattct gcagcaattt
     3601 ggcctcggga ttgtcatcgg acttggtggt ggttatttac tgctgcaaat gattaatcgc
     3661 atcgccctgc ccgccggatt atatccattg ctggcattaa gcggcggtat tttaattttt
     3721 gctttaacca cggcgctgga aggcagcggt attctggctg tttatctgtg cggttttctg
     3781 ctgggtaatc ggccgattcg caaccgctac ggcatcctgc aaaatttcga cggcctcgcc
     3841 tggctggcgc aaatcgccat gttcctggtg ctggggctgt tggttaaccc aagcgatctg
     3901 ctgcctattg ccattccggc gctcattttg tcggcatgga tgatattctt cgcccgtccc
     3961 ctttcggtat ttgctggatt gctccccttc cgcggtttca atctgcgtga gcgcgtgttt
     4021 atcagctggg taggattacg cggcgcggtg ccaatcatcc tggctgtgtt cccgatgatg
     4081 gcggggctgg agaatgcacg tctgttcttt aatgtcgcct tctttgtggt tctggtttca
     4141 ctgctcttgc agggaacatc actctcgtgg gcggctaaaa aagccaaagt ggtggttccg
     4201 ccagtgggac gtccggtgtc acgcgttggc ctggatattc atccggaaaa tccgtgggag
     4261 cagtttgttt atcaattgag tgccgataaa tggtgcgtgg gcgcggcact gcgcgatttg
     4321 catatgccaa aagagacgcg tattgcggca ctgtttcgtg ataaccagtt gcttcatcct
     4381 accggcagca cccgactgcg cgaaggcgat gtgttgtgtg taattggtcg ggaacgcgat
     4441 cttccggcgc tcggtaaact gttcagccag tcgccgccgg tcgcgctgga tcaacgcttc
     4501 tttggtgact tcattctcga agccagcgcc aaatatgctg atgttgcgct gatatatggc
     4561 ctggaagacg ggcgagaata tcgtgataag cagcaaacgc tgggtgaaat tgtccagcag
     4621 ttgttaggcg cggcaccggt tgtcggtgac caggtagagt ttgccgggat gatctggacg
     4681 gtggccgaga aagaagataa tgaagtgttg aagattggcg tacgggtggc tgaagaggaa
     4741 gctgaatctt aaatttaatg aagcatctat cgccggttgc gatgctttgc tgaacgcagc
     4801 ctgagcctgc aattgtttgt cgtccataat ttaaccttca tttgatgttg aattgaacat
     4861 atcaaaatca ggcaattaca caaatctatg tacaggctcc atcaagggta aagcgtgatt
     4921 tatctgaagt cgagttcgag tcaacaatga agcgtaagcg ttttatgtag aacgcctgtg
     4981 cggtgagttc aggttccggg gaaatagtgt ccattctttt aatggacact atcatgggca
     5041 atggctctgt cacattaaca actacagttg ctgaccagcc ggatggatga ttttttcgca
     5101 accagaagac gctgcctccg atccggctta caacaagtta cactgtcaca accgggacgc
     5161 gtaacgccag cgcgcacatc aactcatagc ccaccgttcc ggcagcggcg gcgacatcat
     5221 caattttgat ctccttgccc cacagctcaa ccggcgtacc aatccccgcc tgcgggcaag
     5281 gcgttaaatc gaccgctagc atatccatcg agacggtccc caccgtcatg gtgcgcacgc
     5341 cgtccactaa aacaggggta ccggtaggcg cgtggcgcgg ataaccgtcg gcgtaccctg
     5401 cggcgacaat gccgattcgc tgttcatcgc gcgcagtata gcgaccgccg tagcccacac
     5461 gctcacccgc ttttagcgtc tggacaccaa taatctcact gcttagcgtc atcaccggac
     5521 gtaatccggt attggcgata tcacgccact gaccggacgg cgaagcgcca tacaaaataa
     5581 tgccaggccg aacccagtca aaatgcgctt ccgggtgcca cagagtcgcc gccgaattgg
     5641 acaacgaacg ccgacactcc agcccttccg ccgcctgctc aatacgcgcc atcgcgctgg
     5701 aaattccatc aggatgttcc gcttcggcaa aatgcgacat cagggtcatc tcgccaacat
     5761 tcgccattgc ccgcaactgc tgccagacgg taagcacgcg atcgggctgg aagcccaacc
     5821 gattcatccc actgttcact ttaagataaa tatccaacgg tgcttttagc cgcgcatttt
     5881 gcagtgcttt gagctgccag ttgctgtgta cgcaggtggt caggcggtgc tggtcataaa
     5941 tctccagatc ctgagcatgg aaaaatcctt ccagcatcag gatcggcccc ttccagccgc
     6001 gctcacgtaa cgttattgcc tcttccaggt taagtaatgc aaagccatcg gtggccccga
     6061 gcgcgctcca gatacgctca ataccatgcc cgtaagcgtt cgcttttacc accgaccaga
     6121 cgcgcgcgtg cggcgcggcc tggcggacaa tggacagatt ctgttttaat gcctgcagat
     6181 cgaggctggc ctgtatcgga cgggtcatct cgtttcctta gctgtgtgcg ccatgtaaat
     6241 ggcccggacg tgatggcgta aatccacggc tgtagcgcgc tacgcttaga tcctcatatg
     6301 ggatcgctgg cgtgcgacca gagagcagat cgcttaacaa ctgaccggaa ccgcaagcca
     6361 tcgtccagcc gagcgtgccg tgaccggtat tcagccacag atttttaaag cgtgtacgcc
     6421 cgacaaccgg cgtaccgtct ggcgtcatcg ggcgcaaacc cgtccagaaa gtcgcctgct
     6481 cgacatgacc gccgcgtgga tagagatcgc gaaccaccat ttccagcgtt tcacgacgcg
     6541 gttgcaacag ctcggtgtta aaaccaacaa tttccgccat tccgccaaca cgaatgcggt
     6601 tatcgaaacg ggtaatggcg attttgtagg tttcatcaag aatagtggat actggcgcac
     6661 catcttcctg cgcaattgga atagtcagcg agtagccttt cagcggatgc accggaatat
     6721 caacaatgcc tttgagcatc gccgtcgagt aagaaccaaa cgccatcaca tacgcatcgg
     6781 ccttaatcac ttcatcgcca cacttcacgc cgtagatttg ctcgccgtcg caaagcagtt
     6841 ggtcaacggg cgtattaaag cggaatttaa cccccgcctg ctctgccatc cgcgccagat
     6901 tctgggtaaa cagctggcag tctccggttt catcattggg cagttgcaga ccacccgtca
     6961 gtttgtgcgc tacttctgcc agcgcgggct ccacttccgc caggcggctg gattccagca
     7021 gctgatacgg tacgccggca tcttccagca cggcgatatc gcgggtcgca ttttcatact
     7081 gttgttcggt acggaacagt tgcagcgtcc caccctgacg cccttcatac tgaatattgg
     7141 tttcggcgcg caatgctttc aggcaatcac ggctgtattc cgccagacgc accatccgcc
     7201 ctttgttttc catgtagtgg ctggtgtcgc agttacgtaa catttgccac atccatttca
     7261 actgaaactg cgtaccgtcg agacggaccg ccagcggcgc atggcgctgg aacatccatt
     7321 tgatcgcttt taaaggcaca cctggtgccg cccacggtgc agcatatccg ggggagattt
     7381 gcccggcatt cgcggcgctg gtttccaggg cggcccccgg ctcccgatca atgacggtga
     7441 cctcatgtcc tgcctgattt aagtaccagg cgctggcaac gcctaccaca ccacttccca
     7501 gtatgacaac tcgcatagcc actccgataa cggtagatac agtaaaagaa taataatcta
     7561 attacatctt gataactcag ttgaaaatat tatt
//
LOCUS       DAZGLJ010000210         7591 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000210 DAZGLJ010000000
VERSION     DAZGLJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.210"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(17..229)
                     /gene="yncH"
                     /locus_tag="ACHK49_004011"
     CDS             complement(17..229)
                     /gene="yncH"
                     /locus_tag="ACHK49_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HFO9264559.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
     gene            complement(305..922)
                     /gene="yncG"
                     /locus_tag="ACHK49_004012"
     CDS             complement(305..922)
                     /gene="yncG"
                     /locus_tag="ACHK49_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO9264560.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIAFMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAVCQLPKLQEVLKRNEII"
     gene            1188..2687
                     /gene="ansP"
                     /locus_tag="ACHK49_004013"
     CDS             1188..2687
                     /gene="ansP"
                     /locus_tag="ACHK49_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO9264561.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2802..3863)
                     /gene="yncE"
                     /locus_tag="ACHK49_004014"
     CDS             complement(2802..3863)
                     /gene="yncE"
                     /locus_tag="ACHK49_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO9264562.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            4105..6207
                     /gene="pqqU"
                     /locus_tag="ACHK49_004015"
     CDS             4105..6207
                     /gene="pqqU"
                     /locus_tag="ACHK49_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO9264563.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(6243..6908)
                     /gene="mcbR"
                     /locus_tag="ACHK49_004016"
     CDS             complement(6243..6908)
                     /gene="mcbR"
                     /locus_tag="ACHK49_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO9264564.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(7106..>7591)
                     /gene="curA"
                     /locus_tag="ACHK49_004017"
     CDS             complement(7106..>7591)
                     /gene="curA"
                     /locus_tag="ACHK49_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO9264565.1"
                     /translation="EKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKGIDIYYENVGG
                     KVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKRIRLQGFIIAQ
                     DYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNFGKVVIRVAGD
                     D"
BASE COUNT         1864 a         1810 c         1842 g         2075 t
ORIGIN      
        1 aacgccattt atccttttag catacctgcc ggatattttt atttgcatta atattaattg
       61 catctccaac tccgagcata tccatcatta aaaacttttt gagattatag aatacctgtg
      121 ataacaagtg aaacatctgc acaatgtgaa tagttagttt cttttcagag gaaatgaaat
      181 agacaagctg aatgaagggg agagtgatat aaatcaaaaa acataacatg aatttaattt
      241 attacaccca tcattgtgga taaagttact gaataataaa taacataatc ttctggtatc
      301 ccactcaaat aatttcattc ctttttaata cttcttgtaa tttcggtaac tgacatacag
      361 catcagcgat cgcgctgata ttctgcgcat tatcctgaaa ccattcatgc cccggccccc
      421 atgtgcgcat ggtgcagaga taacagtcca ccagcgttag ttgctcacca aaggcataag
      481 gttcagcggt caattgtgaa ttcagccaga tataaagtga tttgcgatat tcaattacat
      541 tcttctttaa ctgttcaggt gcgtcagatg cccaacgctc agggtaatcg gcgaaagtga
      601 atgttggata gacattagca accagccaga ccaatagccg ttgaaactgc tgacgttcgg
      661 cgcgcccaac cggtggcgca aggtccgggc gacgatcgag aaccataaat gcaatcgccg
      721 ctgtctccgt cataatctcg tcattttcca gcgccagtgt cggaacctga cacaacgggt
      781 tcagggtttt caataactcg cgtgaagctc cctcgtggtc aaaaccactg acatcaacga
      841 actgataagg aatatcagcc agtgttagca tcagctcact gattgtagag ccccatccgg
      901 gtacgccata caccttaatc atatgccctc acaatctttt aaagattaag tgtaggacag
      961 tacacaggtt aaccagcaaa aagatttgtc attttttcgt cagttgtatt attccccttc
     1021 aggattgtaa ggggatgttt tgcatcgttt atcgataacg ggtaggatgc tgcgccgata
     1081 gttttatacc cataaagaat aatggtgata actatcatcg ccaggatgaa taaacattgt
     1141 tcatggcaac ttatatgact ttttcattaa agcaatcagg gagagcaatg agtaaacacg
     1201 acaccgacac ttcagatcaa cacgccgcga aacgccgctg gcttaatgcc cacgaagagg
     1261 ggtatcacaa agcgatgggc aatcgccagg tacagatgat cgccattggc ggcgcgattg
     1321 gcaccggctt gtttttaggt gcaggagccc gactgcaaat ggcgggtcca gcattggcac
     1381 tggtttattt aatttgtggc ttgttttcgt tttttattct gcgtgcattg ggtgagctgg
     1441 tgctacaccg cccttccagt ggcagttttg tctcttatgc ccgtgagttt ttgggtgaga
     1501 aagccgctta tgttgctggc tggatgtact tcatcaactg ggcgatgacg gggattgttg
     1561 atattaccgc cgtcgctctg tatatgcatt actggggtgc gtttggcggc gtgccgcagt
     1621 gggtctttgc gctcgctgca cttaccatcg ttggcaccat gaatatgatc ggtgtgaaat
     1681 ggtttgcgga gatggagttc tggtttgcgc ttattaaagt gctcgccatt gtgacctttt
     1741 tggtcgtggg tacagtgttc ctcggtagtg gtcagccgct ggatggcaac accactggct
     1801 ttcatttaat taccgataat ggcggcttct tcccccacgg tttgctgcct gctctggtgt
     1861 tgattcaggg cgtagtgttt gcttttgcct ccattgaaat ggtgggtaca gctgccggag
     1921 aatgtaaaga tccgcagacc atggtgccta aagcgattaa cagcgtgatt tggcgtattg
     1981 gcctgtttta cgtcggctcc gtggtgttgc tggttatgct attgccgtgg agcgcgtatc
     2041 aggcggggca aagtccgttc gtgacgtttt tctctaaact gggtgtgcca tatatcggca
     2101 gcattatgaa cattgtggtg ctgaccgctg ccctctccag cctgaactca ggtctgtact
     2161 gcaccggacg tattctgcgc tcaatggcga tgggcggttc cgcaccgagt tttatggcga
     2221 aaatgagtcg tcagcatgtg ccgtatgccg ggattctggc gacactagtt gtgtatgtcg
     2281 tcggcgtatt cctcaactat ctggtgccgt cgcgcgtatt tgagattgtg ttgaacttcg
     2341 cgtcgctggg aatcatcgct tcatgggcgt ttatcatcgt gtgccagatg cgcctgcgta
     2401 aagcaattaa acaaggcaaa gcagcggatg tcagttttaa actgcctggc gcgcccttca
     2461 cttcctggct gacattactg tttttactga gtgtcctggt gctgatggcg ttcgattacc
     2521 cgaacgggac ttatactatc gcggcgctgc cgattatcgg tattctgctg gttataggct
     2581 ggtttggtgt gcgcaaacgc gttgctgaaa ttcacagcac tgcgccagtc gtcgaggaag
     2641 atgaagaaaa acaggaaatt gtgtttaagc ctgaaacggc gagctaattg caaaattgcc
     2701 cggctacgtg ccgggcaaat ttagattact gacgaaggtg tgccgcatgc ggcgtgataa
     2761 aagtcaggca agtctctccg ttttcaccct cgccacatct attacagcgc aatacgaatc
     2821 acatcgtccg gctgggtagc ttcctgctgt ttagtggatt tttgtttcac actgacatac
     2881 agcgttttgc catcggcaga cagcgccagg ctgtttggat gagtcggcgt atcgaacgtt
     2941 ttcaccactt tatagctttt cgcgtcaatc acactgactt tacctgcctg acgatgcgtt
     3001 acgtaggctt cattacgcgc tgggttaaac agcacagcca gtgattctgg tgccgcaacc
     3061 ttcgccagaa tattgccatt acgggtatcg accactaaca cttctgcggc tttagaatcg
     3121 gtgataaatg cacgctgcct ggcggtatca aggctaatgt tgataaagaa gtgctctttg
     3181 ccgtcatcca gcagcttttt acggctgagg attttattgt cggcggtgtc gatggtaatc
     3241 aattcgccgt cagcgttagt ggtgtaaaga cgtttgcctt tgctatccag cgccagaccg
     3301 gtactcattt taccggtgtt ctggatggcg gttttcagtt tgatattctc gccatcaacg
     3361 acccaaatca cgctctcttt accaatacca ctgatgtaaa cggtgttcgt ggcatcatca
     3421 gctaccagct cacgcggttg cagcgggcgc acctcttccg tgcgcttacg atcatccagc
     3481 accagacggc ctttcacctc gcccgttttg gcatctatcg ccgtgaccgc gctgtttacg
     3541 gtgttaccaa accacaacgt ctgagtcgtg ttattgatgg tggcaccaaa cggcttgaga
     3601 tcgttatgga tcgcctgcgt cacttccaga gtgaccggat caagacgata aaccacgcca
     3661 cctttatcca gtttgcggct ttgcgaagtg gcgagccaca gtgcgttttc ttgctggcta
     3721 taagccattt cgtaggcacc tttacctacc gctttacgca gcatttcttc tgcggcctgc
     3781 gtactgaatg atgaagcaac aagcaatgaa cctaacagta atgaaccacg caggcgcgat
     3841 gaaaacagat gacgtaaatg catgacgact ccctttgatg aaccgatatt tttgctgctt
     3901 cacaatgctt cgctaagcgt aatcatcgtt acgctcttgt tataaatggg aatggtaatc
     3961 attattttct caaatgtgga gcatttcttg ccgttacgac tacaattttc tcaattactt
     4021 aacgtatcgt gccgtttcgt tttccataaa tttacaattt cattaccata acggcgtttt
     4081 cccattctta acctggcttt tttcatgaag attttttccg tccgacagac cgttttgccc
     4141 gcactgcttg tcctttcccc cgttgttttt gccgctgatg aacagactat gattgtcagt
     4201 gccgcaccgc aggtggtttc agaactggat accccagcag cagtaagcgt ggtggatggc
     4261 gaggagatgc gcctggcaac accgcgcatt aacttgtccg aatcactgac cggcgtgcct
     4321 ggtttgcagg tacaaaaccg gcagaactat gcgcaagatt tacagctgtc gattcgcgga
     4381 tttggctccc gctccactta cggtattcgc ggtattcgcc tgtatgtgga cggtattccc
     4441 gccaccatgc ccgacgggca agggcaaaca tccaacatcg atttaagcag tgtgcaaaat
     4501 gtggaagtgc tgcgtggccc cttctctgcc ctgtatggca acgcgtctgg tggggtaatg
     4561 aatgtcacca cccagaccgg acaacagcca ccaaccattg aagccagtag ttactacggc
     4621 agttttggca gctggcgcta tgggctgaaa gcaacgggcg caacgggaga cggcacacag
     4681 cctggcgatg tcgattacac cgtctcaacc acgcgtttta cgacccacgg ctatcgtgac
     4741 catagtggcg cacagaaaaa tttagctaat gccaaactgg gcgtacgcat tgatgaagcc
     4801 agcaaattaa gtctgatttt caatagtgtg gatatcaaag cagatgaccc aggtgggcta
     4861 accaaagcag aatggaaggc taatccacaa caagcgcctc gtgcagaaca gtacgacacg
     4921 cgaaaaacca tcaagcaaac tcaggctggg ttgcgctatg agcgtagcct gagcgcgcaa
     4981 gatgatatga gtgtgatgat gtatgccgga gagcgagaaa cgacccagta ccagtcaatc
     5041 ccgatggccc cccaacttaa cccgtcacat gcgggcggcg tgattaccct gcaacgccat
     5101 taccagggga tagacagccg ctggacacac cgtggtgaac tgggcgttcc ggtcacgttc
     5161 actaccggcc tgaactacga aaacatgagt gaaaaccgca agggctacaa taacttccgc
     5221 ctgaatagcg gcatgccgga gtacgggcaa aaaggtgagt tgcgtcgcga cgaacgcaat
     5281 ctgatgtgga acatcgatcc ctatttacag acgcagtggc agctgagcga aaaactgtcg
     5341 ctggatgctg gcgtgcgcta cagctccgtg tggtttgatt ccaacgacca ttacgttact
     5401 ccgggtaacg gcgatgacag cggtgatgcc agttatcata aatggctacc tgccggttcg
     5461 ttaaaatatg caatgaccga tgcctggaat atctatctgg cagccgggcg aggttttgaa
     5521 acgccgacga ttaatgagct gtcttatcgt gctgatgggc aaagcggtat gaactttggt
     5581 ttaaaaccat ccaccaacga tacaattgag atcggcagta aaacgcgtat tggtgatggg
     5641 ctgcttagtc tcgcattgtt tcagaccgac actgatgatg aaattgttgt cgatagcagt
     5701 agcggtgggc gtacgactta caaaaatgcc ggaaagaccc gtcgtcaagg cgctgaactg
     5761 gcatgggatc aacgtttcgc aggagatttt cgcgtaaacg cgtcctggac ctggcttgat
     5821 gcgacctatc gcagcaatgt ttgcaatgaa caggattgta acggtaatcg gatgccaggg
     5881 atcgcccgta atatgggctt tgcgtcgata ggttatgtac cggaagatgg ttggtatgca
     5941 ggcacggaag cgcgttatat gggcgatatt atggcagatg atgaaaatac ggcaaaagcg
     6001 ccgtcttata ctctcgtcgg cttattcacc gggtataaat acaattacca caatttaact
     6061 gtggatttat ttggtcgtgt cgataattta ttcgataaag aatacgttgg ttctgtcatt
     6121 gtcaatgagt caaacgggcg atattacgaa ccttcgcccg gacgaaatta tggtgtcggc
     6181 atgaatattg cgtggagatt tgagtaatta ccttgatgcc cggtatttgc cgggcattta
     6241 ctttaacgat tgtattgctg gtataaaata gcaatatttt gttgcataat ttcagcaaga
     6301 caatgtctgc atccctcttt gtcttttgct tttaacgcag cgagtaacag atgatagttt
     6361 tctatatgct cccgtaattc cgctggatta atcgcttcat agagataatg taaaccaggc
     6421 cccatcctga cccacagttg ctcaatcatc tcacacagga tgggcatatt tgagcgatga
     6481 taaatcgcta agcgaaatag cctgtttaca ttaatgattt gttccatgtc gcccttttct
     6541 tgcgcctgct gtaatttctc gagcaattcc tggagttccg ccaggtcttg cggggtgagg
     6601 ttttcaacag ccagagcaac tgccattaat tccagttcgt agcggatccg attgatttcg
     6661 tctaattgac gtttcctcac ttccggaact gtaaatgctt gtgcaggtgc gacagaaagc
     6721 gcattcaccg aaaccagacg taataatgct tcacgcacag gtgtaatact catacctaat
     6781 tgctccgcca gatttttagt aatcaggcgt gcgccaggtt ttaatgcgcc aatactaagc
     6841 tgatgtttca ggtcgttctc aacctgcaga gtcaaactga catgtttcat ttttcccatt
     6901 ccaggcattt taattccttc gcgtgttttc tgtccaaccc tgcctgcacg gcagaaggag
     6961 ttcacctggt gtattaaagt gaataatcat aaaccaccca cctttgttgt tattagcagc
     7021 agaacaatga cgtggttagt taattaaagc tgaaaataag ctttttgcct ggatttttat
     7081 ggcggcttac gccgccgttg aaactttaat catcacccgc gacgcggatc accactttgc
     7141 cgaagttttt acccttcagc aggccgataa acgtctgtgg cgcattctct aaaccatcag
     7201 taatatcttc gtggtagtgg attttatcct ctttcaccca ttgccccatc tccttctgaa
     7261 actcatggat gcggtgacca taatcctgag cgataataaa cccttgcaag cgaatacgtt
     7321 ttttcagcac cgttgccatc aacaggggta aacgatccgg accgggtggt agctctgtag
     7381 cgttatagct gctcactaag ccacaaacgg gaatgcgcgc agatgtatta agtaatggta
     7441 gcactgcatc aaatacctta ccgcccacgt tttcataata gatatcaata ccttttgggc
     7501 acgctttcac cagttgttcg gcaaaatcat ccgcgtggtg atcaagacaa acatcgaagc
     7561 ccagcacctc gatagcatgg cggcattttt c
//
LOCUS       DAZGLJ010000211         7575 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000211 DAZGLJ010000000
VERSION     DAZGLJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.211"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            156..986
                     /gene="yjiC"
                     /locus_tag="ACHK49_004018"
     CDS             156..986
                     /gene="yjiC"
                     /locus_tag="ACHK49_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO9264566.1"
                     /translation="MSMPLSNTLQSQIITDNHFLHHPKVDSELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVENYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1127..1900)
                     /gene="uxuR"
                     /locus_tag="ACHK49_004019"
     CDS             complement(1127..1900)
                     /gene="uxuR"
                     /locus_tag="ACHK49_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO9264567.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDV"
     gene            complement(2115..3575)
                     /gene="uxuB"
                     /locus_tag="ACHK49_004020"
     CDS             complement(2115..3575)
                     /gene="uxuB"
                     /locus_tag="ACHK49_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO9264568.1"
                     /translation="MTTIVDSNLPVARPSWEHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFSLMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKVNAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3656..4840)
                     /gene="uxuA"
                     /locus_tag="ACHK49_004021"
     CDS             complement(3656..4840)
                     /gene="uxuA"
                     /locus_tag="ACHK49_004021"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO9264569.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5292..5384
                     /locus_tag="ACHK49_004022"
                     /pseudo
     CDS             5292..5384
                     /locus_tag="ACHK49_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5627..6529)
                     /gene="fimH"
                     /locus_tag="ACHK49_004023"
     CDS             complement(5627..6529)
                     /gene="fimH"
                     /locus_tag="ACHK49_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO9264570.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGSVLSNFSGTVKYSGSS
                     DPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTVDAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6549..7052)
                     /gene="fimG"
                     /locus_tag="ACHK49_004024"
     CDS             complement(6549..7052)
                     /gene="fimG"
                     /locus_tag="ACHK49_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO9264571.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7065..>7575)
                     /gene="fimF"
                     /locus_tag="ACHK49_004025"
     CDS             complement(7065..>7575)
                     /gene="fimF"
                     /locus_tag="ACHK49_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO9264572.1"
                     /translation="LLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAESTNFTVDL
                     MENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLALENTVSA
                     ASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPVTAGHINA
                     TATFTLEYQ"
BASE COUNT         1873 a         1976 c         1864 g         1862 t
ORIGIN      
        1 tactttagag tgtttcctac cctttaatat caccgtgtag agttgatatc actacaacag
       61 aaacatgcat gtcatttgtt tacttctcat tttactacag aaaaatttat ctatttttct
      121 aaacatcagt gaaatactca taaaaggtaa ctattatgtc tatgccatta agcaataccc
      181 ttcagagcca aattattaca gataatcact tccttcatca tccaaaagtc gacagtgagt
      241 taacacgtaa atatgaacgt gcacgcctgg atacggaaaa tatctatctt ctgcctcttg
      301 ctcgtggcaa taaccataat tacgatggaa aaagcgttgt cgaaattaga aaacttgata
      361 ttagtaaaga gtcctggcca tttaattatg tgacagaagc atgcagagaa tccgatggta
      421 ttacaacaac aggacgtatg ctctatcgaa acttgaaaat cacctcagct ttagacgaaa
      481 tatatggtgg catatgtaaa aaggcgcatg ctgctacaga attagcagaa ggtttacggc
      541 taaatttatt tatgaagagc cctttcgatc ctgttgagaa ctatacagtt catgaaataa
      601 cattaggccc tggttgcaat gttccaggtt acgccggaac cacgatagga tatatttcaa
      661 cccttccagt ttctcaggca aaaagatgga ccaatgagca gccaagaatt gatatctata
      721 ttgatcagat aataactgtc tcaggggtag caaattcttc cggattcgcc cttgccgcat
      781 tacttaatgc taatattgag atgggaaatg acccaatcat tggtatagaa gcctatcctg
      841 gtaccgcgga gatccatgca aaaatgggat ataaagttat ccccggtgat gaagatgcgc
      901 cactcaaacg catgactctg caaccgtcat ctcttccaga actgtttgaa ttaaaaaatg
      961 gtgaatggaa ttatattggt aaatagccac tgagaagaat gagtactaat attcaggtta
     1021 tacagatatt taaacaatac cctgcaattt acaaagcaat actcattgcg acttcttaat
     1081 ttaagaaagc gcaaggaacg tttacccttg cgcttattat aataagtcag acgtcgactt
     1141 tatccagcgg ccatgaatca aacagatagc cgtcaaaata aatatcgtca acgttcgaga
     1201 attccagcag acgttgctta acgttttcca gatgctgcca cattgccagc ttcgccgctc
     1261 gggcatcttt tttgattaac gcggcgagga tctgtttgtg atcgcccaac cactctttgc
     1321 gatacaggct gtcatccaga tggctgtgca actggatcca cattggattg ttttcccgcc
     1381 actgccagga ctgacggaat aactccacca gcatgctgtt atgcgttgct tcggcaatag
     1441 cgagatggaa ctgcatgtca ccgctttcgc tgctgcccgg cgcactggaa gccagttcac
     1501 gctcttccag ttgcaatgcc tgacgcattt tgacgatatc ttcgcgggta gcctgcaaag
     1561 cggcaaactc ggcgatgttg ctctccaata actgccgcgc ctgtaacagc tcaaaaggac
     1621 cggcatcgtt gcagacgttg gcatccggac tgtctgtgtt ctggctgcct gagttatcaa
     1681 gaacatagat accggcaccc cggcgtactt ccaccagccc tttgatctcc agcatgatca
     1741 gcgcttcacg taccaccgtc cgcgtgacat caagcatttc tgcaatttca cgctccggcg
     1801 gcagccgttc gccaggattg tacggcgtct ttatgatcag atcgcggatc atcgccccga
     1861 cttcctggta aggtctttgc gcagaggtgg cagatttcat ggtgttgtgt ccgttaggtg
     1921 cggttaatca aatttttgcc ggatgcggcg tgaacgcctt atccggccta caacgggcaa
     1981 ttttacggcc cccgttaatc tcaaactata ccattagcac tggaagttgg cacgtacagg
     2041 tgggcaaaaa aacgaaaact ggtcaaccaa atatcagttt gattgaccag taagtctgta
     2101 gggtaatcag ttagttacag cgcagccaca cactcgcgcg caccgcgttc gcacagctgc
     2161 tgatatgccg ccgtcactgc gccaacaaag tcggcattct gcggcagatc atcggcaaaa
     2221 ataccgctca ggcccagcag cgctttcacg cggtctgcgc cctgatactg cgcgttgacc
     2281 ttctggaact ccgccagcat cgggtcgacc acgtcaatgg cattaccctg ctcatccacg
     2341 ccctgggtgt aacgcatcca gccagccacg cccagcgcca ggtgacgcca gctgccgccg
     2401 ttttgcaggt gcagacgcac cgggtccagc agacgctgcg gtaacttctg gctgccgtcc
     2461 atcgcaatct gccaggtacg gtgacgcaga gacgggttgc tgaaacgctc gatcagcagc
     2521 gtcgcatagg cgttcaggtc cgtaccttcc ggcatcgaca gcgttggcgc ttgttcctgc
     2581 atcatcaagg agaaggccgc tttgcgataa tccgggttag tcatggtgtc ggcaatggtt
     2641 tcatagccgc cgaggtaacc gaggtatgcc aggaaagagt ggctgccgtt cagcatacgc
     2701 agcttcatca tttcgaacgg cacaacgtct gcaacgaact gtgcgcccac tttatcccag
     2761 tccgggcgac cgttaacgaa gttatcttca atcacccact gacggaacgg ttcgcaggca
     2821 atggcgcacg ggtcgtaaac acccagctgg tcagcaattt cctgcaaggt ttctggcgtc
     2881 gccgccggaa cgatgcggtc aaccatggtg cacgggaagg tgacgttttc ttcaatccat
     2941 gccgccagct gcgggtcacg cgcctgagcc agtcccagta ccgcgacctt cgccacatgg
     3001 ccgttttcac gcacgttatc gcaggacatc accgtaaacg ctttcaaccc tttttcacga
     3061 cgcagccgca gggcttcgac gatgtaacca atcgcggact tcggcgcagt cgggttttcc
     3121 agatcgtgct tgatcagtgg gttattgaga tccagctgac cgcttgccgc atcagcgcag
     3181 tagccttttt ccgtgactgt cagagagaca atcgccgtct gcggacgcgc catcgcattg
     3241 agaatacctt cgcagccgtc gatttccgga tgcagcgctt ctttcatcga accgataatt
     3301 ttcagctcgg tgctttccgc gcctttttcc gccacggtgt acagcagttg ctgttttttc
     3361 aggttttcga tcagcacgcg gtcgttgcct ggcatcaggt taacttcgca gataccccag
     3421 tcgctgtcgg tgctttccag caggtgatgg gtatacagcg cctggtgcgc gcggtgaaac
     3481 gccccgcaac cgagatgcac aatgcgtgat tccagacgag aatgctccca tgacgggcgg
     3541 gcaaccggca gattgctgtc aacaatagta gtcatcgtaa actccagaat ccgtcttcag
     3601 agagtgacgg caaggaagaa ggaaccggca cggcgcgcag ccatgccggt gggtattaac
     3661 ggctaaagaa agcgcgctgg atcgccagtt cgacaccgcg aacttcggcc aggcctttca
     3721 gacgaccaat tgcggagtaa cctgggttgg ttttcttctt caggtcgtcc agcatctgat
     3781 gaccgtggtc cggacgcatc gggatcaggt cttctttgcc ttccgcttta cgacggtgtt
     3841 cttcttcaac aatcgctttc accacttcgt acatatcaac gtcaccgttc aggtgcgccg
     3901 cttcgtggaa ggttttcggg ttatcttcac gcatggtgga gcgcagatgg gtgaagtaaa
     3961 tacgcggacc gaactgcttg atcatatcaa ccagatcgtt gtcagcacgc acgccgtagg
     4021 aaccggtgca catggtgaaa ccgttcgcca tgctgtttac ggtatcaacc atccactgca
     4081 tatcttcaat ggtggaaaca atgcgcggca ggccaaggat cgggcgcggc ggatcgtccg
     4141 ggtgaaccgc catacgcacg ccaacttctt cagcaactgg aataatcgct ttcaggaaga
     4201 cggcaaagtt ttcgcgcagc ttcgctttat cgatatcttt gtacagctcc aggtgcttac
     4261 ggaactggtc gagggtatac ccttcttccg cgcccggcag accggcaatg atgttacgcg
     4321 tcagacgcgc tttgtcttcg tcgctcatag tggcgaagcg aacagcagcc tgagcaattt
     4381 cttcttcggt gtaatccgct tccgcgcctg gacgtttcag gatatgcatt tcgaatgcag
     4441 cgaattcgat ctggtcgaag cgcagagctt tggagccgtc tggcagcacg tattcgaggt
     4501 cagtacgggt ccagtcgagc accggcatga agttgtagca cacggtgcga atgccgcact
     4561 gcgccaggtt gcgcagagtc tgctgatagt tagcgatcca ctgctcatag ttgccagtgt
     4621 gggttttgat atcttcgtga attggtacgc tttctacgac agaccacacc aggcctgcgt
     4681 cttcaacgat cgccttgcgt ttgaggatct cttctacgga ccatacttcg ccgttcggga
     4741 tatggtgcag cgcggtaacc acgccagttg cgcccgcctg acggacatca gctaaagaaa
     4801 ccggatcgtt tgggccgtac cagcgccagg tctgttccat gatttttcct ctttcacata
     4861 gtgggttcca cagcaaagag acgcggaacc ctggagttgt ttaggtatcc ggatgaacaa
     4921 ccagaccgca gccgtggcgt tgcatcggat gagatggcgt ataagttcta ccttttggta
     4981 agacatgtca acccgaattc tgaaattggt taaccacatc acaagaattt cactctcatg
     5041 gttgttgctg caaattgcag caaagggtaa tcgatggtta ttgatcagag gatgtgacat
     5101 tcatcgcaac aatggttgac caatttacat aacatgtcgc ccaaaataac aacggttcaa
     5161 ccaggtaagg taaaacgtta tgcatgtgct taacattctc tgggtggtat tcggcattgg
     5221 cctgatgctg gtactgacaa agaccgcagg ttcagacaca ttcgctgcat tagcaatgcc
     5281 tcttagataa ttttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     5341 tggtcaactc cgtggttggg ctgattatta gcaaggtagc gtaaaagaac accagagcct
     5401 gccaatggca ggctctgact gatgacaaag tctaaaaatg tgcccggaca gtcccctcgc
     5461 ccctccgggg agagggttag ggtgagggga acagaccagc aaagtgcgct ttgtttatgc
     5521 cagatgcgac gctgacgcgt cttatctggc ctacaaaggg ctaacgtgca ggtttttagc
     5581 ttcaggtaat attgcgtaac tgcattagca atgccctgtg atttctttat tgataaacaa
     5641 aagtcacgcc aataatcgat tgcacattcc ctgcagtcac ctgccctccg gtacgtgcgt
     5701 aatttgccgt taatcccaga cttaccgccg aagtccctac tgctcctaac gataccgtgt
     5761 tattcgctgg aataatcgta ccgttgcgcg tcaactgtac gccgacgccc tgcgctggtg
     5821 aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc tacggttgtg ccggagaggt
     5881 aataccccag attttggctt ttcgcacaat aaacggtaag aggaattggc actgaaccag
     5941 ggtagtccgg cagagtaacg gtgacatcac gagcagaaac atcgcagccg ccagtaggca
     6001 ccaccacatc attattggcg taaatattcc acacaaactg aaaatcatcg ctgttatagt
     6061 tgttggtctg tcgcaaaata agcacggcaa ttaatgagcc agctttaatc gccaccccgc
     6121 ccgcactgct cacaggcgtc aaataaagcg ccaccggcca cggcttatcc gttctcgaat
     6181 tataaacaac gcgcggcgtt tcgctggtgg taggaaatgg atcgctactg ccactatatt
     6241 ttacggtccc ggaaaaatta gataacacgc tgccataagc cgagcctcgt tgcagtgtga
     6301 catagtctgt aatggtttcc ggataatcgt tatggcaaaa gatttgcgtc gaaagatcca
     6361 cgaccaggtt ttgccccaca ttcacggcgg gcgcaaggtt tacataaaca ttggcgctgc
     6421 caccgccaat agggatagcg gtaccattgg cggttttaca ggcgaatgac caggcattta
     6481 ccgaccagcc catcagcagt acagcaaaca gggtaataac tcgtttcatt acaatcatct
     6541 cttcgggttc agctgtaggt ataggtgatg ctaatcactg cctgaatggt tccctgagtg
     6601 gctccgccat ttaccgtcaa tgctctgacc tgtaacggga agtgcgctga ttgtgaggaa
     6661 tcatccacct gaactgtttt ggttgcgcca gtattcaatg tgttgccact gtcatcctgt
     6721 agctctaact ggatgttttg cgcggtcccc tggtttttat aatatccggt actgtcggct
     6781 gccccgctga agctggcggt gaccctcgac gttcccaccg gacaattagt caactcaagc
     6841 gcaacatcat gccaggccga tgccgccccg gcagacatca gactgaaaga ataaagatcg
     6901 ccgagatcaa ccgtggcatt ggtggtggaa accgtacacg gtttggcgac gaccttaccg
     6961 ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac ttgcgagcgc caatattgcc
     7021 gccaataaat acccacgttt gcgccatttc atgagcatct ccagttactg atattcaaga
     7081 gtgaaggtag ccgtggcatt gatatgcccc gcagtgacag gcacctgtgt cgccattagc
     7141 cgggcgtaaa aattcaacgt atttggttta cccggcgtca gggtcgtcca cgaaatcgcg
     7201 gacgatggag cattaagggg tatttgattt tgctgctcat tcagaagctg tattcccagt
     7261 cccgaagccg ctgacaccgt attttcaagt gcaagcagat tggcattgtg gctatctgca
     7321 acgccggtaa acccaacttt tacggcagaa acggcattac cacagggtga cagcaaaata
     7381 cgaaatggaa caacaggagt cgtcgcgcca atgttgttaa attgcttcgc cgcgttttcc
     7441 atcagatcaa cagtaaaatt ggttgattca gcggccacac tacagccgtt atctctgaca
     7501 tagccgcgga tagtaatcgt gctatccgca gccaaagcgt gactcaccgc cagccacaaa
     7561 aaagcgcaca gaaga
//
LOCUS       DAZGLJ010000212         7564 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000212 DAZGLJ010000000
VERSION     DAZGLJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.212"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            33..635
                     /gene="yebB"
                     /locus_tag="ACHK49_004026"
     CDS             33..635
                     /gene="yebB"
                     /locus_tag="ACHK49_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO9264573.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(637..1158)
                     /gene="ruvC"
                     /locus_tag="ACHK49_004027"
     CDS             complement(637..1158)
                     /gene="ruvC"
                     /locus_tag="ACHK49_004027"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO9264574.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1193..1933)
                     /gene="yebC"
                     /locus_tag="ACHK49_004028"
     CDS             complement(1193..1933)
                     /gene="yebC"
                     /locus_tag="ACHK49_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9264575.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1962..2414)
                     /gene="nudB"
                     /locus_tag="ACHK49_004029"
     CDS             complement(1962..2414)
                     /gene="nudB"
                     /locus_tag="ACHK49_004029"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO9264576.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2532..4304)
                     /gene="aspS"
                     /locus_tag="ACHK49_004030"
     CDS             complement(2532..4304)
                     /gene="aspS"
                     /locus_tag="ACHK49_004030"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO9264577.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4614..5180
                     /gene="yecD"
                     /locus_tag="ACHK49_004031"
     CDS             4614..5180
                     /gene="yecD"
                     /locus_tag="ACHK49_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO9264578.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5177..5272
                     /locus_tag="ACHK49_004032"
                     /pseudo
     CDS             5177..5272
                     /locus_tag="ACHK49_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5498..5746
                     /locus_tag="ACHK49_004033"
     CDS             5498..5746
                     /locus_tag="ACHK49_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO9264579.1"
                     /translation="MRVEICIAKEKISKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(5743..5829)
                     /locus_tag="ACHK49_004034"
                     /pseudo
     CDS             complement(5743..5829)
                     /locus_tag="ACHK49_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            5914..5997
                     /locus_tag="ACHK49_004035"
                     /pseudo
     CDS             5914..5997
                     /locus_tag="ACHK49_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            complement(6118..7131)
                     /gene="nleC"
                     /locus_tag="ACHK49_004036"
     CDS             complement(6118..7131)
                     /gene="nleC"
                     /locus_tag="ACHK49_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFO9264580.1"
                     /translation="MYKTILSINMKIPVLQPGFNFFAPAGYSAAVAPNRAENAYADYV
                     LDIGKRIPLSAADLSNVYESVISAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDA
                     VSYGIHNEEVHIGCIKYRNEYELNGESAIKIDDIQSLTCNELYGYDVGQEPILPICEA
                     GENENEEPYVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGASDPSGDSNIELGPTEIL
                     ARRVAQELGWSVPDFKGYAEPEREAHLRLRNLNALRQAAMRHEKNERAFFERLGTISD
                     RYEASPDFTEYSAVSNIGYGFIQQHDFPGVAIDENLQDVNQIQLYHGAPYIFTFGDVD
                     RHN"
BASE COUNT         1880 a         1972 c         1747 g         1965 t
ORIGIN      
        1 tatattcata tctatttcaa tggaatgaca acgtgaatat taattaccct gctgaatatg
       61 aaattggtga tatcgtcttt acatgtatag gtgctgcctt atttggtcaa atatcagctg
      121 catcaaactg ctggagtaat cacgtcggga tcattatcgg tcataacggt gaagactttc
      181 tggttgcaga aagccgtgtt cccctctcaa ccatcactac gctatcccgt tttattaaac
      241 gctctgctaa tcaacgctat gctataaagc gattagacgc cgggctgaca gaacaacaga
      301 aacaacgaat tgttgaacag gttccctccc ggctacgcaa actttaccac acgggtttta
      361 aatacgaatc ttcccgccag ttctgttcaa aatttgtctt tgatatttat aaagaggcgc
      421 tatgtattcc ggtgggtgaa atagagacgt ttggacaact gttaaatagc aatccgaatg
      481 caaaactcac tttctggaaa ttctggttct taggttctat tccgtgggag cgtaaaaccg
      541 tcacgccagc cagtttgtgg catcatccgg gtttggtgtt gattcacgcg gagggagttg
      601 aaacgcctca gcctgaactg accgaggcgg tataacttaa cgcagtcgcc ctctcgccag
      661 gttcagccgc gattcgctca tctgcattgc attctggctg acgtgacaat gggtaatcgc
      721 gatagctaac gcatcagcgg catccgcttg tggattagcg ggcagtttca gcaaggtgcg
      781 gaccatatgc tgcacctggc ttttctcggc gctaccaata cctaccaccg tttgctttac
      841 ctgacgtgcc gcgtattcaa ataccggcaa ttcctgattc accgccgcca caatcgccac
      901 gccgcgcgcc tggcccagtt tcagggctga gtcagcgttc ttcgccataa agacttgttc
      961 aatggcgaaa taatcaggct ggaactgggt gatgatttcc gtcacgcccg catagatgag
     1021 cttcagacga gacggtaaat catccacttt ggtgcgtatg catccactac ccaggtagga
     1081 cagttgcctg cctacctggc ggatgacgcc atagccggtc acgcgcgaac ccggatcaat
     1141 gccgagaata atagccatca cgcgtctccg ttttgctgtt tagcaggcct catcagagag
     1201 tcgctgcaac ctcatcagag atttcaccgt tatggtaaac ttcctgcacg tcgtcgcaat
     1261 cttccagcat atcgatcaga cgcatcagtt tcggtgcggt ttctgcatcc atatcagctt
     1321 tggtggacgg gatcatggaa acttccgcgc tgtctgcttt cagacctgcc gcttccagag
     1381 cgtcgcgcac tttacccatt tcttcccatg cggtgtagac atcaatcgcg ccgtcatcat
     1441 aggtcacaac gtcttcagca cctgcttcca aagctgcttc catgatggtg tcttcatcgc
     1501 ctttctcgaa ggagatcacg ccttttttgc tgaacagata ggctacggaa ccatcagtac
     1561 cgaggttacc gccacatttg ctgaatgcat gacgcacttc agcaacggta cggttgcggt
     1621 tgtcagacag acattcaatc atgattgccg tgccgccagg accgtaacct tcgtagatga
     1681 tggtttccat gtttgcatca tcatcaccgc ccacaccacg tgcaattgcg cggttcagtg
     1741 tgtcgcgggt catgttgtta gacagtgctt tatcaattgc tgcacgcaga cgcgggttag
     1801 cgtccggatc gccaccgccc agcttagccg cggttaccag ctcacgaatg attttagtga
     1861 agattttacc gcgctttgca tcctgcgcag ctttacgatg tctggtgttg gcccatttac
     1921 tatgacctgc cataaaaata tctccagata gccctgcctg ttcaggcagc gttaatgaca
     1981 aactgttcaa tcgcctgccg gttgctccag gacttagtga gcgccgccgc agcaggcgca
     2041 tcaagccact tgtaagccag atgttcagtg aaaacgatct gccgctcgtg cggaagcgca
     2101 agacagaacc atgattccgt attacgcgtc acgcccggcg catagcgatg acgtaaatgt
     2161 gaaaaaattt caaactctac cgtgcgctga cagtcaatta aggtcagttg ttcagcgaca
     2221 acatcaatgg tgacctcttc ctttacttcg cgcatggcag cttgcggcgc ggtttcaccc
     2281 tcttccacgc tgccggttac cgactgccag aaatcgggat cgtcacgccg ctgcaacatc
     2341 agcacccgtt tcgtatcttg tgcatagatg accactaaga tcgaaacggg acgcttatac
     2401 actttatcct tcacgctgcc tcttcgttga ctgccttcgc tcatcccatt cacatagtta
     2461 tctatgctca tgggagttca ctcagttgcc gccttgatgc aacgcgaatg atttcgtgta
     2521 tttgagtcat atcagttatt ctcagccttc ttcacaacct gaatgctcag ctcagccagt
     2581 gcagtcgggt tagcaaagct cggtgcttca gtcatcagac acgctgccgc cgtggttttc
     2641 gggaaggcga taacgtcacg gatattgtcg gtgccagtca gcagcatggt cagacggtca
     2701 agaccgaatg ccagacctgc gtgcggcgga gtaccgtatt tcagggcgtc gagcaggaag
     2761 ccgaatttct cgcgctgttc ctcttcgttg atacccagaa taccaaacac cgtctgctgc
     2821 atatcaccat tatggatacg tactgaacca ccgcccactt cgtaaccatt gatgaccata
     2881 tcgtaagcgt tcgccaccgc attttccggt gcagctttca gttctgcagc agtcatgtct
     2941 ttcggtgagg tgaacggatg gtgcattgcc gtcaggccgc cttcaccgtc gtcttcaaac
     3001 atcgggaagt cgataaccca cagcggtgcc catttgcttt cgtcggtcag accaaggtct
     3061 ttacccactt tcaggcgcag tgcgcccatc gcgtcggcaa caattttctt gttgtcggca
     3121 ccgaagaaaa tcatatcgcc atcttgcgcg gcagtacgat ccaggatggc ttcgatgatt
     3181 tctgcattaa ggaacttcgc taccgggctg ttgatacctt ccagaccttt cgcgcgttcg
     3241 ttaactttga tgtaagccag acctttcgcg ccgtagattt taacgaagtt accgtattcg
     3301 tcgatctgct tacgggtcag cgatgcgcca cccggaacac gtagagcggc aacacggcct
     3361 ttcggatcgt tcgccggccc cgcaaatact gcaaactcaa cagatttcag cagatcggca
     3421 acgtcggtca gttccatcgg gttacgcaga tccggtttat cagaaccata acggcgttct
     3481 gcttccgcaa aggtcattac cgggaaatcg cccagatcca cgcccttcac ttccagccac
     3541 aaatgacgca ccagcgcttc catcacttca cgcacttgcg gcgcggtcat gaaagaagtt
     3601 tccacatcga tctgagtaaa ttcaggctga cgatcagcgc gcaggtcttc gtcacggaag
     3661 catttaacga tctgatagta acggtcaaaa ccggacatca tcagcagctg tttgaacaac
     3721 tgtggggatt gcggcagtgc gtagaattta cctttgtgca cacgagaagg caccaggtag
     3781 tcacgcgcgc cttccggcgt ggctttggtc agcatcggag tttcgatgtc gaggaagccg
     3841 tggtcatcca taaaacggcg caccaggctg gtgattttag cgcgggtttt caggcgctga
     3901 gccatttccg gacgacgcag gtcgaggtag cggtatttca gacgcgcttc ttcggtgttg
     3961 acgtggttag agtcaagcgg cagaacatct gcgcggttga tgatagtcag cgaggacgcc
     4021 agcacttcga tttcgccggt cgccatatcg cggttaatat ttttttcgtc acgcgcacgt
     4081 acggtgcccg tgacctgaat gcagaactca ttacgcagtt cagaggccag ctttaacgcg
     4141 tccgcacgat ccggatcgaa aaatacctgc acgatacctt cgcggtcgcg catatcgatg
     4201 aagatcaggc taccaagatc acgacgacgg ttgacccaac cacacagagt cacctgctgc
     4261 cccacgtggg acaaacggag ctgtccacaa tattctgtac gcatgagata tcccttaact
     4321 tagctgccgg cggatgcccc ctgctgcgca ggtgaccaag tcgcagcgtt agctgtatgt
     4381 cacaactgaa tgaaaaaagg cggctattat actggaaatt ctgccgcacc gtaagagcct
     4441 ggcccgcgct ggaacgcctc gttaccactt tatatcgggc ctgaaatcag actctacgcc
     4501 agtttgctat aaaggtgttg cccgaactca taaaaattaa caaaatttgt cgttccgcca
     4561 tcggctaatc gcattaaggt gtgaggcacg attttgtttt ttcaggagtc attatgcttg
     4621 aacttaatgc taaaaccact gcgctggtgg tgatcgattt acaagaaggc atcttacctt
     4681 ttgccggtgg tccacatact gccgatgagg tagttaatcg cgccgggaag ctggcggcga
     4741 aatttcgcgc cagcggtcag cccgtgtttc tggtgcgcgt tggctggtct gccgattacg
     4801 ccgaagcatt aaaacagccg gttgatgccc cctcccccgc taaagtgttg cccgaaaact
     4861 ggtggcaaca tccagctgca ttaggtgcaa ctgacagtga tatcgaaatc atcaaacgtc
     4921 agtggggtgc gttttacggt acggatctgg agttgcaact gcgccgccga ggtatcgata
     4981 caatagtgtt atgtgggatc tcgaccaata tcggtgttga atccaccgcc cgcaatgcct
     5041 gggaacttgg ctttaatctg gtgattgccg aagacgcctg tagtgccgct agcgccgagc
     5101 agcacaataa cagcatcaac catatctacc cacgcatcgc ccgtgtgcgt agcgtggaag
     5161 agatccttca cgcgttatga tttacatcgg tctaccgcaa tggtcgcatc ctaaatgggt
     5221 gcggttgggg atcaccagcc ttgaagagta tgcccgccac tttaactgca tgacgcaaca
     5281 tttttaaaaa tcgacgaaga tcgtccagga gcgccgtttt tcaagggttg gatagatgat
     5341 tcgtttacca tgtcgacgaa cttcgacgaa tttcgccgat aaaaagtgtc ccatacatgc
     5401 cccattgatg ccccataaac aatatcagga attgacactg gttatccata cagataaaaa
     5461 taacacctgt atataaccac agttacagga gggtgttatg cgtgttgaga tctgtattgc
     5521 taaagaaaaa atcagcaaaa tgccaaacgg tgctgtggat gcgttaaagg aagaattaac
     5581 ccgacgcatc agtaaacgtt atgacgatgt agaggtgatc gtaaaagcca ccagcaacga
     5641 tgggctttct gttacacgca ccgcagataa agattctgca aaaacatttg ttcaggagac
     5701 tctgaaagat acctgggagt ctgctgacga gtggtttgtt cactaataaa cacgtaaaat
     5761 cggtaacggc tggaaatcat tcaatactcg cactatcgaa agttcaccag ccaaccgcga
     5821 cacgctctta catacgaagt gccgcgcttt ccttaataat tttttagcag tactgtgtaa
     5881 atgatgagcg acctaatcca tgcatgacgc gtagtgccta ttgtgcatct ttctgcgtct
     5941 ctttttactg gacccaagcc aatgatgctg gcgataccat tcccgccagg cccacttaca
     6001 acagtaaaat tcggagctct gcctttacat acaatacaat gttgcacgat tgcccatcat
     6061 aaactccgtt aatatctatg agtagtaacc ctatgtaact gtaaagacga atcagcgcta
     6121 attgtgtctg tccacatccc caaatgtaaa gatataagga gcaccatgat acagttggat
     6181 ctgatttaca tcctgtaaat tctcgtcgat agccacccct ggaaaatcat gttgctggat
     6241 aaatccgtat cctatgttag acacagcgga atactctgtg aaatcaggac tcgcctcata
     6301 tcggtcactg atcgtaccca gtctttcgaa gaaagccctc tcattctttt catgcctcat
     6361 ggcagcctgt cgaagggcat tcaggttacg tagtctaaga tgagcttctc gttctggctc
     6421 tgcatatcct ttgaagtcgg gaacactcca tcccagctct tgagcgacac gacgtgcgag
     6481 aatctcggtg ggtcctagct ctatattact atctccagat ggatcgctgg ccccagtaac
     6541 atgatgaata atctcgtgaa tcagtccttc ctgccacgat ggcatctcat aagaatcagt
     6601 atctggcgca acactaaaac tgacataagg ctcttcgttc tcgttttctc ctgcctcgca
     6661 aatggggaga attggttctt gcccgacatc gtatccatat aattcgttac aggttagtga
     6721 ttgaatatca tcaattttga tagcagattc tccgttaagc tcgtattcgt ttctgtattt
     6781 aatgcaacca atgtgtacct cctcattatg aatgccatag cttacggcat cacgaaatgt
     6841 ttgtgatcgg ctcaacgcat caagtacagt gttgccaatc atatcgaccg tatgctgatc
     6901 gatgagcctg ctacggctgt catggacggc gctaattaca ctttcgtata cgttgcttaa
     6961 atctgctgcg gaaagcggta ttcgcttacc tatatccaaa acgtaatccg cataggcatt
     7021 ttcagcacga ttaggagcaa cggcagcaga gtatccagca ggggcaaaaa agttgaagcc
     7081 aggttggaga acgggaattt tcatattgat acttaatatg gttttatata tttcagtgcc
     7141 accaattcac tcgcttacaa ccagaaacta aaaaaacgtc agggcgttga taataatgaa
     7201 taccaacttt ctgcccattt atagtaatat ctttcgttat caggttttgt ctggcagctt
     7261 cacgtaatat ttcaatactc tgcaaagtaa catttgatga aatgctggat gttatcaatg
     7321 gcatatgtca tttaccccaa caagactaaa ttttagaaaa aacaactgcc gggacaactt
     7381 tcgccccctg taaccccaaa taagatgtca gatttatcgg cattaattta tctccctgtt
     7441 gaattagcac ctctatcatc tacttaacaa atatcccacg gacattgcaa cacaaaaacc
     7501 ggagccggac tccggttttt gtgaagctgt cgggctattt catcccgcca acattttccc
     7561 acgt
//
LOCUS       DAZGLJ010000213         7545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000213 DAZGLJ010000000
VERSION     DAZGLJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.213"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(229..933)
                     /locus_tag="ACHK49_004037"
     CDS             complement(229..933)
                     /locus_tag="ACHK49_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO9264581.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1070..1522)
                     /gene="moaE"
                     /locus_tag="ACHK49_004038"
     CDS             complement(1070..1522)
                     /gene="moaE"
                     /locus_tag="ACHK49_004038"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO9264582.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1524..1769)
                     /gene="moaD"
                     /locus_tag="ACHK49_004039"
     CDS             complement(1524..1769)
                     /gene="moaD"
                     /locus_tag="ACHK49_004039"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO9264583.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1762..2247)
                     /gene="moaC"
                     /locus_tag="ACHK49_004040"
     CDS             complement(1762..2247)
                     /gene="moaC"
                     /locus_tag="ACHK49_004040"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO9264584.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVTALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2250..2762)
                     /gene="moaB"
                     /locus_tag="ACHK49_004041"
     CDS             complement(2250..2762)
                     /gene="moaB"
                     /locus_tag="ACHK49_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO9264585.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2784..3773)
                     /gene="moaA"
                     /locus_tag="ACHK49_004042"
     CDS             complement(2784..3773)
                     /gene="moaA"
                     /locus_tag="ACHK49_004042"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO9264586.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3761..3905)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4170..5078
                     /gene="yvcK"
                     /locus_tag="ACHK49_004043"
     CDS             4170..5078
                     /gene="yvcK"
                     /locus_tag="ACHK49_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO9264587.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5270..7291)
                     /gene="uvrB"
                     /locus_tag="ACHK49_004044"
     CDS             complement(5270..7291)
                     /gene="uvrB"
                     /locus_tag="ACHK49_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO9264588.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         1827 a         1991 c         1882 g         1845 t
ORIGIN      
        1 gctgttttgt gagtatgatt ccattctttt agttgtcctt agaaaaaata ttaatattca
       61 ccatcaataa atttaaaacc acgcatacaa aatacttttg tatttatgga tacggaaaac
      121 gcttccgctc gccataaggc gtattttata cttggtactt gttaagtgtc gtttttagta
      181 agaaaaaaaa tcccgacaca tggccgggat attgatggtg aaaaagaatt aacggcggtt
      241 gccaaaaatt cgcagcaaca tcaggaacag gttgatgaag tccagataca aggttaacgc
      301 gccaagaatg gaatatttgc gcaggttcga cgtgtcgcgg gtatcaatct gctcacccat
      361 atttttcaat ttctgagtgt catacgccgt caatccgaca aagacaatca cgccgatgta
      421 ggtaactgcc cacatcaatg cttcgctttt cagccagaag ttgaccagcg atgccagcac
      481 aatgccgatt aacgccataa acagcatatt gccaaagcca cttaaatcgc gcttcgtggt
      541 gtaaccgtac aggctcattg cgccgaacat cccggcagta acgacgaaag tactggcgat
      601 agaagcagcg gtatagacaa tgaatatact ggaaagcgta agacccgtca gcgccgaata
      661 aagcataaag agcatcgtcg ttacacctgc gctcagcttt tgaatcatcg ctgataacac
      721 aataaccaat gctaattgcg cgatgatcag accgattaaa aagacacggt tagtaaacaa
      781 cagctccatc acggccgcgg aattagccgc ataccaggca acaaatgcgg tcagcaacaa
      841 gccaacggtc atccagccat agacttgcgc catataagtt tgcaagccag cccggggttg
      901 tacgattgaa tcagaacgtg ggaatctgtc catgacgatc tcctgaagat ataaggaata
      961 tcttaaggat actgcaaaat gatgaggctg tgcatcgacg cagcgtaaac gcatgtactg
     1021 agcggtgaaa ttgccggacg cagcggtgcc ttatccggct aacaaaaaac taccagcgtt
     1081 ttgccgcctg ctgatcgctc tcccgagctt caacccagcg gtcgccttcc ggcgtggctt
     1141 cgcgcttcca gaacggtgcg cgggttttga gataatccat aataaactgc ccggcttcaa
     1201 acgcactgct gcgatgcgca ctggtgacac cgacaaaaac gatttcatcg cccggccata
     1261 attccccgat gcggtgaatc acagtgacgc gccccagcgg ccagcggtta cgcgcttcat
     1321 caacaatttc tgccagtgct ttttcagtca tccccggata gtgttcgagg gttaatgctt
     1381 tgacgctgtc gcccaggtta tggttgcgca ccttaccagt aaaggtgact accgcaccgt
     1441 cttcgtcacg ctccgccagc cacgggtact cttctcctac gctgaacggc tgcggaccaa
     1501 caacaatttt ggtttctgcc atcttaacct ccggttaccg gcgggaagaa agctacttcg
     1561 tcgccgtcag tcagcggatg gtcaaaactc accagcgtct ggttgacggc agccagtaat
     1621 ttgccatctt ccagcgccag cgcccagcga tcgctctgcg cagccatgtg ctggcgtaac
     1681 gcttcaacag ttgggaaatc cgcagccact tcggttgcat ctgttcccac caactcgcgc
     1741 acctgggcga aaaaaagaac tttaatcatc cgcttccacc ttaaagtcac ccgacttgcc
     1801 gccgctcttc gccagcaaac gtaccggacc aatcaccata tctttttgca ccgctttgca
     1861 catgtcataa atggtcagcg ccgtcacgga ggccgcggtt aatgcttcca tttcgacacc
     1921 ggttttcccg gtcaggcggc ataaggtttc tatacgcacc cgattgtgct ccggctcggc
     1981 ctgtaaattg acttcaactt tgctgagcat cagcggatga cagagcggga tcagatccca
     2041 ggtgcgtttt gccgcctgaa taccggcaat acgcgcagtg gcaaatacgt cgcctttgtg
     2101 gtggcgacca tcaataatca tcgccagcgt ctcgctgcgc atggtgacaa aggcttccgc
     2161 ccgcgcttca cgcacggttt ccgctttggc ggagacatcc accatgtgcg cttcgccagc
     2221 ggcgttgata tgggtcagtt gcgacatact tatttcttca aatgtggatg gaaattacac
     2281 ggacgcgtac gggcatccag ctgcggcgcg atgatatttt cccatgcggt acggcacgct
     2341 ttggtcgaac ccggcatggc gaaaatcagc gttttgttgg cgacgcccgc taccgcacga
     2401 gattgcaacg tggaagtgcc aatctcttca aacgacaaca tacggaacac ttcaccaaaa
     2461 ccttcaattt cacggtcgaa caacggcagc aatgcttcgg gagcctgatc accttccgtc
     2521 aggccagtac cacccgtaat caataccact tgtacatcgt cgctggcgat ccacgcagat
     2581 acctgagcgc gaatagcgta gcggttttct ttcacaatgg ctttatcgac aacgtgatgg
     2641 cccgcttctt gcgccgaatc gcgcagatag tgaccggagg tatcgtcttc ttcaccgcga
     2701 cgattagaaa ccgtaagaat agcaatacgg gtcgggataa attcagtgct tacctgactc
     2761 atctgatctc tccttttgac gttttagccg ccaatgtacg ataagttttg cgtaataccg
     2821 gtgttgtttt gatgcaggaa atgggtctgt ttcttctccc gcagcgccgc tgaaatacgc
     2881 gcttccagcg cctgttgctg ggtatcgtct tccagcagat cgcgcaggtt aacgccgcct
     2941 tcaccaaaca ggcagagatg gagtttacca atggaggaaa cgcgcaggcg gttgcaagtg
     3001 gcgcagaagt ctttttcata cggcatgata aggccaatct ctccggcgta atctggatgg
     3061 caaaagactt gcgcgggacc gtcgctgcgt tgacgtaatt ggtggatcca gccgcgacgc
     3121 agtagctcgt cacgcagaac ctgaccagag atgtgatgct tacggaagag ctcgctgccc
     3181 tcgcccgttt ccatcagttc gatgaaacgc agctggatag ggcgatgctg gatccagttc
     3241 agaaaggtgt cgagctggtg atgattaaca tcacgcatca gcacggtatt gactttgacc
     3301 ttctcaaaac cggcctcaaa tgcagcatca atccccgcca tgacctggtt gaatttatcc
     3361 tgcccggtaa tagcgtgaaa ctggcgggcg tccagactgt cgacactgac gttaatgcca
     3421 gtaagtcccg catcgcgcca gttcgccaca tcgcgttcca gacggtaacc attggtggtg
     3481 accgcaatct ggcggatagc gtcgttttcc cgcacagcgg cgatgatatc ggtaaagtcg
     3541 cggcgtaaag acggctcacc gcctgtcagg cgcacttttt cggtgcccag actggcgaag
     3601 gcgcgcgtaa cccggcgaat ttcatcgacg gtaagaaagc ctttattggt gacgccgctc
     3661 ggtttgtagc catccggcag gcagtaggtg caacgaaagt tacacacatc ggtaatcgac
     3721 aggcgcaagt agtaaaactt acgcgcaaat gcatcagtca gttgtgaagc catgtacacc
     3781 tttccagata cgggaggcga agtcatttct tccttcgccc tggtggcaat ctttacgagc
     3841 attgtcacgg ccaaagcacc gtatcagttg acccaggtgc agaggctaga gtgtttagtg
     3901 gttatgccga tactagcgtg taaatgagat ttttaccatc cacactttcg ctatgtaatc
     3961 atgtatatag cgtcatgatc gtgctatttc gctacagaat aagggaaaat ggcggtttta
     4021 ctttgatata catcatcagg gaggcatagc gtgcatcgtc ctttagaggt agaagaaaat
     4081 agtttgtatc ctcaatattg gcaggttaat tgctgtttcc ccgcaatttg cgctactgta
     4141 gcgcggataa tctgactcca ggaaactata tgcgcaatcg tacgctggct gatcttgatc
     4201 gtgtcgttgc tctcggcgga gggcatggac tgggacgcgt tctctcatca ctttcgtctt
     4261 tgggttctcg tttaacgggt atcgtcacca ccaccgataa tggtggctcg acggggcgta
     4321 ttcgccgttc agaaggcggc attgcctggg gcgatatgcg caactgcctc aaccagctga
     4381 taacggaacc gagcgtcgcc tccgcgatgt ttgaataccg ttttggtggc aatggcgaac
     4441 tttccggtca taatctcgga aacttgatgt taaaggcgct ggatcacctt agcgtgcggc
     4501 ctctggaagc catcaattta attcgtaatc tgctgaaagt ggatacgcat ttgattccaa
     4561 tgtcagagca tcctgttgat ctgatggcga ttgacgatca ggggcatgaa gtttacggcg
     4621 aggtcaatat cgaccagtta actacgccga ttcaagagtt attgttaacg cctaatgtac
     4681 ccgcaacgcg tgaggcggtt cacgctatca atgaagcgga tctcatcatt attgggcctg
     4741 gcagttttta taccagcctg atgccaattc tgctgctgaa ggaaatcgcc caggcattac
     4801 gccgcacgcc agcgccgatg gtttatatcg gcaatctggg gcgtgagttg agtttacctg
     4861 cggctaattt gaagctggaa agcaagctgg caattatgga gcagtatgtt ggtaaaaagg
     4921 tcattgatgc ggtcatcgtc gggccaaaag tggatgtctc ggcggtgaaa gagcggattg
     4981 tgatccagga ggtactggag gccagcgata ttccgtatcg tcatgaccgc cagttgttac
     5041 ataacgcgct ggaaaaggcg ttacaggctt taggttaacg gaaatccgaa ggaaaattcc
     5101 ggcttcctat tgaagacaaa gtgcgcgttg tttatgccgg atgcggcgtg aacgccttat
     5161 ccggcctaca aaccgcgcaa attcaatata ttgcggagaa aatgtaggcc tgataagcgt
     5221 agcgcatcag gctgttttcc gtttgtcatc agtcttcttc gctatcctgt tacgatgccg
     5281 cgataaacag ctcacgcagc tgatgcaact ggtcacgaat ttgcgccgct tcttcgaact
     5341 ccagattctg cgcgtgttgc atcatcaacc cttccagctc atggattttc tgctgcaacg
     5401 ctttaggcga catatccatc ggcacattat ccggctcaac aatcggtcgc gattttcctc
     5461 tgcccttcgc tttggtttta gcaatgttct gccccaacgc caggatatcg accactttct
     5521 tgttcaagcc ttgcggcgta atgccgtgtt cctcgttgta cttctgctgt ttctcgcggc
     5581 gacgttcggt ttcgccaatc gctttcgcca ttgatggggt gatcttatcg ccgtagagaa
     5641 tcgctttacc gttaacgtta cgtgccgcac gaccaatggt ctggatcaac gaacgttcgg
     5701 aacgcaggaa gccttctttg tcagcgtcga ggatcgccac cagcgaaact tccggcatat
     5761 ccagaccttc acgcagtaag ttgatcccta ccaatacgtc gaactcaccc agacgcaagt
     5821 cgcggataat ctccatacgc tcgacggtgt cgatatctga gtgaagataa cgcacgcgct
     5881 caccgtgttc ttcgagatat tcggtaagat cttccgccat tcgcttggtc agtgtggtga
     5941 ccagtacgcg ttcgttaatt gccgctcgct gacgaatctc cgaaagaaga tcatcaacct
     6001 gtgtcgccac cggccgcact tcgataatcg ggtcaagcaa tccggttgga cgcaccacct
     6061 gatccaccac atcgccgccg gatttttcca gctcgtaatt acccggcgtc gccgaaacat
     6121 agatggtttg cggcgctaat gcttcgaact cttcaaactt aagcggacgg ttatccagcg
     6181 ctgatggcag gcggaagccg tactccacca gtgtctcttt acgcgcccgg tcaccgcgat
     6241 acatgccgcc aatttgtgga atggtgacgt gagattcatc gaccaccagc agcccatcag
     6301 cagggaggta atcaaacagc gtcggcggtg gctcacccgg tccacgaccg gagaggaagc
     6361 gcgagtagtt ttcaatcccc gaacagtagc ccagctcgtt catcatctcc agatcaaact
     6421 gggtacgctg ggtcagccgc tgctcttcca gcagtttgtt gttttccaac agcactttgc
     6481 gtctggcggc cagctcttct ttgatctcct ccatcgcctg tacgatgcgc tcgcgcggtg
     6541 tgacgtagtg cgttttcggg tagatggtaa aacgtggaat agtggaaaca atctgcccgg
     6601 tcagcgggtc aaataacgac aatcgttcca cttcctcgtc aaacagttcc acgcgaagtg
     6661 caatgtcatc cgattctgcc gggaagatat ctatcacctc gccacgaacg cggaaagtac
     6721 cacgctggaa tgcttgatca ttacgagcgt attgcagctc cgccagtcgg cgcagaatcg
     6781 cgcgctgatc gataatcata ccgaccgtga gatggagcat catcttgaga tataaatcag
     6841 gatcgcccag accataaatc gcggaaacag acgccaccac aaccacatca cgccgctcca
     6901 gcatcgcttt ggtggcggac aaacgcatct gctcaatatg ttcgttaacc gaggcatctt
     6961 tctcaatgaa agtgtcggaa ctcggtacat aggcttccgg ctgatagtag tcgtagtagg
     7021 aaacgaaata ttccaccgcg ttttccggga agaactcttt catttcgcca tacagctggg
     7081 ccgccagcgt tttgttgggc gcaagtacca tggttgggcg ctgaaggtca gcaatgacat
     7141 tggcaatggt gaatgttttc ccggagccag tcacgccaag taacgtctgg tgcgccaggc
     7201 catcttccag cccctcttcg agacgtcgaa tcgcctctgg ctgatcgcca gaaggtttaa
     7261 aagcggaatt cagtttgaac ggtttactca tgagtcgcta cctgaaggag ttgggcgggc
     7321 aggtatgtaa ttttactcgt cgtacttaat tatgccaaca aattatactg gataaaaaaa
     7381 cagttcatca ccataatatt tctgttacag cgtaaactcc gctgtcaatc atgagcaaaa
     7441 tttactctgt ggcgagataa aactccggcc ttaccgggtt atccccaaag caacggcttt
     7501 tttaacattt gtcaagatga gtgatgacag ttttatggca agaga
//
LOCUS       DAZGLJ010000214         7481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000214 DAZGLJ010000000
VERSION     DAZGLJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.214"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..847
                     /gene="gatY"
                     /locus_tag="ACHK49_004045"
     CDS             <1..847
                     /gene="gatY"
                     /locus_tag="ACHK49_004045"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO9264589.1"
                     /translation="VSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAPVII
                     AGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVMIDA
                     SHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQAREF
                     AEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDIQQT
                     IKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIADCG
                     CEGRA"
     gene            876..2138
                     /gene="gatZ"
                     /locus_tag="ACHK49_004046"
     CDS             876..2138
                     /gene="gatZ"
                     /locus_tag="ACHK49_004046"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO9264590.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIESTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            2148..2600
                     /gene="gatA"
                     /locus_tag="ACHK49_004047"
     CDS             2148..2600
                     /gene="gatA"
                     /locus_tag="ACHK49_004047"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO9264591.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVYDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            2631..2915
                     /gene="gatB"
                     /locus_tag="ACHK49_004048"
     CDS             2631..2915
                     /gene="gatB"
                     /locus_tag="ACHK49_004048"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO9264592.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            2919..4274
                     /locus_tag="ACHK49_004049"
     CDS             2919..4274
                     /locus_tag="ACHK49_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO9264593.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            4322..5362
                     /gene="gatD"
                     /locus_tag="ACHK49_004050"
                     /pseudo
     CDS             4322..5362
                     /gene="gatD"
                     /locus_tag="ACHK49_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
     gene            5468..6241
                     /locus_tag="ACHK49_004051"
     CDS             5468..6241
                     /locus_tag="ACHK49_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO9264594.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(6323..7222)
                     /gene="yegS"
                     /locus_tag="ACHK49_004052"
     CDS             complement(6323..7222)
                     /gene="yegS"
                     /locus_tag="ACHK49_004052"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO9264595.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         1882 a         1680 c         1944 g         1975 t
ORIGIN      
        1 ggtatcgaca aagcagatgc tgaacaacgc acagcgcggc ggttatgcgg ttccggcatt
       61 caatattcac aatctcgaaa cgatgcaagt ggtggtggaa accgctgcca acctgcatgc
      121 gccggtcatc atcgccggaa cgcctggcac atttactcat gctggtacag aaaatctgtt
      181 ggcgctggtc agcgcgatgg cgaagcaata tcaccatcca ctggcaattc atctcgacca
      241 tcacacgaaa tttgacgata tcgctcagaa ggttcgttct ggcgtgcgct cagtcatgat
      301 tgacgcctcg catttgcctt ttgcgcaaaa tatttcacgg gtcaaagagg tggtggattt
      361 ttgccatcgc tttgatgtca gcgtcgaagc ggagctgggg caacttggcg gccaggaaga
      421 tgatgtgcaa gtcaatgaag ccgatgcttt ttataccaac cccgctcagg cgcgtgaatt
      481 tgccgaggca accggaattg attccctggc ggtcgccatc ggcacggctc atgggatgta
      541 tgccagcgca ccggcgcttg atttttctcg actggagaac attcgccagt gggtgaactt
      601 accgctggtg ctgcatggcg cgtcagggtt atcgactaag gatattcagc aaaccatcaa
      661 actggggata tgcaaaatca acgttgcaac ggagctgaaa aatgccttct cgcaggcgtt
      721 aaaaaattac ctgaccgagc accctgaagc gaccgatccc cgggattatt tgcagtcggc
      781 taaatccgca atgcgcgatg tggtgagcaa agtgattgcc gattgtggct gcgagggcag
      841 ggcataacgc acctgccatt taataaggaa aaaacatgaa aacgttaatt gcccggcata
      901 aagctggtga acatatcggc atatgttcag tctgttctgc ccatccgttg gttatcgaag
      961 cggcgctggc atttgatcgc aacagcacgc gcaaagtgct gattgaagca acgtcaaacc
     1021 aggtcaatca atttggcggt tataccggaa tgacaccggc agactttcgc gaatttgttt
     1081 ttacgattgc cgataaagtt gggtttgcac gcgaacgcat tattctcggc ggcgatcatc
     1141 tggggccaaa ctgctggcag caagaaaatg cggatgcggc gatggaaaaa tccgtcgagc
     1201 tggtaaagga atatgttcgt gccggtttca gtaaaattca tcttgatgcg tcaatgtcct
     1261 gcgcggggga tcccataccg ttagcaccag aaacggttgc ggaacgagct gctgtgcttt
     1321 gctttgctgc ggaaagtgtg gcgacagatt gccagcgtga gcaactgagc tatgtcattg
     1381 gcaccgaagt tccggttccg ggcggtgagg ccagcgccat tcagtcagta cacatcaccc
     1441 atgttgaaga tgccgccaat actttacgta cgcatcaaaa ggcctttatt gcccgtgggc
     1501 tgacagaggc gttaacacgc gtgattgcca tcgtggtgca gccgggcgtg gaatttgatc
     1561 acagcaatat tatccattat cagccgcagg aagcgcagcc gctggcgcaa tggatagaaa
     1621 gcacccgaat ggtttatgaa gcacattcta ccgattacca gacccggacg gcttattggg
     1681 aattagtccg cgatcacttt gcaatattga aagtcggtcc cgcattaacc tttgctttac
     1741 gcgaggcgat atttgcactg gcacaaattg agcaggaact tatcgcccct gaaaatcgca
     1801 gcggttgcct ggcggtaatt gaagaagtga tgctcgacga accgcaatac tggaaaaaat
     1861 attatcgtac gggttttaac gattcattac tggatattcg ttacagcctg tcggatcgta
     1921 ttcgttatta ctggccgcat agtcggatta aaaatagcgt cgaaacgatg atggtgaatc
     1981 ttgaaggcgt ggacatccca ctgggcatga ttagtcagta tcttcccaaa caatttgaac
     2041 gcattcagtc cggggaatta tcagcaatac cgcatcagct gattatggat aaaatttatg
     2101 atgttttgcg cgcctatcgc tacggctgtg cggaataagg acggtatatg actaacctgt
     2161 ttgttcgtag cggaatttct tttgtcgatc gtagcgaagt tttaacccat atcggtaatg
     2221 agatgctcgc gaaaggtgtg gtttatgata cctggccaca ggcattaatt gccagagaag
     2281 cagaattccc taccgggata atgcttgagc agcacgccat tgctataccg cattgtgagg
     2341 cgattcatgc taagtcgtca gccatttatc tgttaaggcc aacaaataaa gttcattttc
     2401 agcaagcgga tgatgataac gacgtggagg tgtcgttggt tattgcgttg attgtggaaa
     2461 atccgcagca gcaattgaaa cttttacgct gtttatttgg caagttacaa cagcccgata
     2521 tcgtcgagac actaattact cttcctgaaa ctcagttaaa ggaatacttc acaaagtatg
     2581 ttttagattc agacgaataa atccctctgt aacaataaaa aggactattt atgaaacgca
     2641 agattattgt cgcttgcgga ggcgcggttg cgacctctac gatggcggcg gaagaaatta
     2701 aagagttgtg tcagaatcat aatattcctg ttgaattaat ccagtgtcgg gttaatgaaa
     2761 tagaaaccta tatggatggt gtgcatttga tatgcaccac tgccaaagtg gatcgtagtt
     2821 ttggcgatat tccgttagtt cacggcatgc cttttatttc tggtgtcggt atcgaagcat
     2881 tacaaaataa aattctgact atcttacagg ggtgacctat gttttcagaa gtcatgcgtt
     2941 atattctcga cctcggccct acggtgatgc ttccgattgt catcattatt ttttctaaaa
     3001 tattaggcat gaaggcaggc gattgcttta aagcgggtct gcatatcggg attggctttg
     3061 ttggcattgg ccttgtgatt ggcttaatgc tggattccat tggtccggcg gcgaaagcga
     3121 tggcggaaaa tttcgacctg aatctgcatg tggtcgatgt cggctggccg ggctcttcac
     3181 caatgacctg ggcgtcgcaa attgcgctgg tggcgattcc gattgcgata ttggttaacg
     3241 tggcgatgtt actgacccgt atgacgcggg tggtaaatgt tgatatctgg aatatctggc
     3301 atatgacctt caccggcgcg ttgctgcacc tggcaaccgg ttcatggatg atagggatgg
     3361 caggtgtggt aattcacgcg gcgtttgttt ataagctcgg cgactggttt gcccgcgata
     3421 cccgaaattt ctttgagctg gaaggtattg ctattccgca cggtacgtcg gcgtatatgg
     3481 ggccgattgc ggtgctggtc gatgctatca tcgagaaaat cccaggcgtt aaccgaatta
     3541 aatttagcgc cgacgatatt cagcgcaaat ttggtccatt tggcgagcct gtcaccgtgg
     3601 gttttgtgat ggggctgatt atcggcatcc tcgcgggtta cgatgtcaaa ggtgtattgc
     3661 agctggcggt aaaaacggcg gcagtgatgc tgctaatgcc acgggtgatt aaacccatca
     3721 tggatggttt aacgcccatc gctaagcagg cccgtagtcg tttacaggcg aagttcggcg
     3781 gtcaggagtt cctgattggc ctggatcccg cattactgct ggggcatacg gcggtggtat
     3841 cggcaagcct gatttttatc ccgctcacca ttttaattgc tgtttgtgtt ccgggtaatc
     3901 aggtgctgcc gtttggcgat cttgccacca tcggcttctt tgtggcgatg gcagtcgccg
     3961 tgcatcgtgg aaatctgttc cgcaccttaa tctcgggtgt catcatcatg agcatcactc
     4021 tgtggatcgc gacgcaaact attggtttgc acacccaact ggcggctaat gctggggcgt
     4081 taaaagccgg gggtatggtg gcttcaatgg atcagggcgg ttctcccatt acctggttac
     4141 tgattcaggt tttctccccg caaaatattc ccggattcat tattatcggc gcaatttatc
     4201 ttaccggtat tttcatgacc tggcgtagag cgcgtggctt tattaaacaa gagaaagtcg
     4261 ttctcgcaga ataattttta cctgaggggg aggttatccc ccttaacaat caggagtttt
     4321 tatgaaatca gtggtgaatg atactgatgg tatcgtgcgc gttgcagaaa gcgtcattcc
     4381 tgaaattaaa catcaggatg aggtgcgggt aaaaattgcc agctcgggct tatgtggttc
     4441 cgatttaccc aggatattta aaaatggtgc acattattat ccaataacgt taggccatga
     4501 atttagcggc tatattgatg cggtgggatc cggtgttgat gatttacatc ctggcgatgc
     4561 ggttgcctgt gtgccgttat taccctgttt tacttgtcca gagtgtttga aagggtttta
     4621 ttcccagtgc gcaaaatatg attttattgg ctcacggcgt gatggtggat ttgccgaata
     4681 tattgtcgtt aagcggaaaa atgtctttgc tctacctact gatatgccta ttgaggatgg
     4741 ggcttttatt gaaccgatta ccgttggcct gcatgctttt catttagcgc aaggttgtga
     4801 gaataaaaac gttattatta ttggtgccgg aaccattggc ctgctggcca ttcagtgcgc
     4861 tgtcgcgctg ggagcaaaga gtgtgacggc gatcgacatt agttcagaaa aactggcact
     4921 ggcaaaatct ttcggtgcga tgcaaacatt taacagtagc gaaatgagcg cgccgcaaat
     4981 gcagagcgtt ttacgctaac tgcgctttaa tcagcttatc ctcgagacgg ctggcgtacc
     5041 gcaaactgtc gaactggcgg tagagattgc cggtcctcat gcccaactgg cgctggtggg
     5101 cacgttgcat caggatctgc atttaacatc ggcaacgttt ggcaaaatat tgcgtaaaga
     5161 gctgacggtt atcggcagtt ggatgaacta ctccagccct tggccggggc aggagtggga
     5221 aacggcgagc cggttgctga cagaacgtaa gttaagcctg gagccattaa tcgctcaccg
     5281 tggaagcttt gaaagcttcg cccaggcggt gcgtgacatc gctcgtaatg ctatgccggg
     5341 caaagtgttg ctcattccct gaaaccgcgg gccagcggga tgctggcccg gtattgtgca
     5401 aaacagatca ttcaccaatg gtcccccttc gtttacacta gccacaattg aatatgggta
     5461 aatgacgatg aattcattcg agcgaaggaa taagatcatc caattagtga atgaacaggg
     5521 aaccgtgctt gttcaggatc tggcgggagt atttgctgcc tcggaagcga caatccgtgc
     5581 cgatttgcgc tttctcgaac aaaaaggcgt ggttacgcgc tttcatggcg gtgcggcgaa
     5641 aataatgtcc ggtaatagtg aaaccgagac ccaggaagtc gggtttaaag agcgatttca
     5701 gctcgccagc gcgccgaaaa acagaatagc gcaggcggca gtcaaaatga tccatgaagg
     5761 gatgaccgtt attctcgaca gcggaagtac cacaatgctt atcgccgaag gattaatgac
     5821 cgccaaaaat atcacggtga ttaccaacag tctcccggct gcgtttgccc tttccgaaaa
     5881 taaagacatt actctggtcg tctgcggtgg cacggtccgc cataaaacgc gatctatgca
     5941 tggatctatt gctgaacgtt ccctgcagga tattaatgcc gatttaatgt ttgtcggtgc
     6001 tgatggtatt gatgcggtta atggtattac gacctttaat gaaggttatt cgattagcgg
     6061 tgcgatggtc accgccgcta ataaagtgat tgccgttctc gattcatcga aatttaaccg
     6121 tcgcggtttt aatcaggtac tgccaatcga aaaaattgac attattatta ccgatgatgc
     6181 tgtatcagag gtggataaac tggcattgca gaagacacgg gtaaaattaa ttacggtgta
     6241 gtattgttct gtatgccgga taaggtattt acaccgcatc cggcatttcg gattctccct
     6301 ggttcaagag agaatagttt gattaacgca ataatggaca atctggtggt aatcgacaac
     6361 gcaacgccgc cggaagtatt tcaatatgaa agttttgtcc gctcaacggt tcgccatcaa
     6421 gattaaaagt gatttcgtgt ggggcttgta tatcaaacca cgacgaagcg ccttcgataa
     6481 tattcgggtt atcttcgtca gattttaagg ttgatacgag agccggaaga atttcatcgc
     6541 cggtaaaaat gcgcagttgc agcaagccat cgttaattaa cgcgttcgga cacaattgct
     6601 gaccgccacc ggcctgacgc ccgttaccaa taccaatgac cagggcgtca ccttgccagt
     6661 gaaagttttc accgcggatt tcacaacggt ccggttgcag agtatccatg cgcattaagc
     6721 catgaatgat gtaagagacg ccacccagcg cggcttttaa tttttccggc gtttctgtgg
     6781 taatacgcgt cccaaatccg cctgtcgcca tattaataaa acaggtttgt ttgttgacct
     6841 gcgccatatc tatcgcaatg gcgttaccgg caattgccag tttcagcgcc ttatccagtg
     6901 cctcaggaat ccctacactg gtggcaaaat cattggcggt tcctaatggc aaaattccca
     6961 gcgcgggtat gtcatccccc tcacactgaa tcaacgccgt agaaacttca ttaatggtgc
     7021 catcgccacc accggcaatc accgttgcga cgcccaactt ccgggcctcc tctacatatc
     7081 gtgcggcatc gcctttctcc caggtgaccc gcacatggat cgtcattcct tcctcacgca
     7141 acagcataat tgcttcgcgc aagggtagat tgtcagtact tttgccatta agaatcagta
     7201 agctggcggg aaattctgcc atgatcgtat gtgcctttat gattggtctg tagatagtgt
     7261 agagcagaaa acaaaaggtg gagtcagaac aggatgaaag tcggaggata aaaagtcagt
     7321 tcaagtatgg aggattcagt ggttaccacc aggagcttgc aatggggaca gcgaatacta
     7381 acggctgcca ccgataaatt tcaaaaaaga gcatatgcct aatattcaac caaacagtgg
     7441 catcttaaat atactattcc aaatatatta aagcccccat g
//
LOCUS       DAZGLJ010000215         7446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000215 DAZGLJ010000000
VERSION     DAZGLJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.215"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            254..1513
                     /gene="codB"
                     /locus_tag="ACHK49_004053"
     CDS             254..1513
                     /gene="codB"
                     /locus_tag="ACHK49_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO9264596.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            1503..2786
                     /gene="codA"
                     /locus_tag="ACHK49_004054"
     CDS             1503..2786
                     /gene="codA"
                     /locus_tag="ACHK49_004054"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO9264597.1"
                     /translation="MSNNALQTIINARLPGKEVLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(2826..3725)
                     /gene="cynR"
                     /locus_tag="ACHK49_004055"
     CDS             complement(2826..3725)
                     /gene="cynR"
                     /locus_tag="ACHK49_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO9264598.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            3835..4494
                     /gene="cynT"
                     /locus_tag="ACHK49_004056"
     CDS             3835..4494
                     /gene="cynT"
                     /locus_tag="ACHK49_004056"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO9264599.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            4525..4995
                     /gene="cynS"
                     /locus_tag="ACHK49_004057"
     CDS             4525..4995
                     /gene="cynS"
                     /locus_tag="ACHK49_004057"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO9264600.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            5001..6182
                     /gene="cynX"
                     /locus_tag="ACHK49_004058"
     CDS             5001..6182
                     /gene="cynX"
                     /locus_tag="ACHK49_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO9264601.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVSWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     V"
     gene            complement(6261..>7446)
                     /gene="lacY"
                     /locus_tag="ACHK49_004059"
     CDS             complement(6261..>7446)
                     /gene="lacY"
                     /locus_tag="ACHK49_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO9264602.1"
                     /translation="GAYFPFFPIWLHDINHISKSDTGIIFAAISLFSLLFQPLFGLLS
                     DKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGIYLGFCFNAGAPAV
                     EAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGIMFTINNQFVFWLGSGCALILAVL
                     LFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKLWFLSLYVIGVSCTYDVFDQ
                     QFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPLIINRIGGKNALLLAGTIM
                     SVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCFF
                     KQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFTLSGPGPLSLLRRQVNE
                     VA"
BASE COUNT         1696 a         1946 c         2016 g         1788 t
ORIGIN      
        1 ggcctgaacc gtaggtcgga taaggcgctc gcgtcgcatc cgacaccccg cgccgatgcc
       61 tgatgcgacg ctgacgcgtc ttatcagtat ctacccacta cccactgttt ttacaccgat
      121 aatttttccc ccaccttttt gcactcattc atataaaaaa tatatttccc cacgaaaacg
      181 attgcttttt atcttcagat gaatagaata cggcggattt tttgggtttc aaacagcaaa
      241 aagggggaat ttcgtgtcgc aagataacaa ctttagccag gggccagtcc cgcagtcggc
      301 gcggaaaggg gtattggcat tgacgttcgt catgctggga ttaaccttct tttccgccag
      361 tatgtggacc ggcggcactc tcggaaccgg tcttagctat catgatttct tcctcgcagt
      421 tctcatcggt aatcttctcc tcggtattta cacttcattt ctcggttaca ttggcgcaaa
      481 aaccggcctg accactcatc ttcttgctcg cttctcgttt ggtgttaaag gctcatggct
      541 gccttcactg ctactgggcg gaactcaggt tggctggttt ggcgtcggtg tggcgatgtt
      601 tgccattccg gtgggtaagg caaccgggct ggatattaat ttgctgattg ccgtttccgg
      661 tttactgatg accgtcaccg tcttttttgg catttcggcg ctgacggttc tttcggtgat
      721 tgcggttccg gctatcgcct gcctgggcgg ttattccgtg tggctggctg ttaacggcat
      781 gggcggcctg gacgcattaa aagcggtcgt tcccgcacaa ccgttagatt tcaatgtcgc
      841 gctggcgctg gttgtggggt catttatcag tgcgggtacg ctcaccgctg actttgtccg
      901 gtttggtcgc aatgccaaac tggcggtgct ggtggcgatg gtggcctttt tcctcggcaa
      961 ctcgttgatg tttattttcg gtgcagcggg cgctgcggca ctgggcatgg cggatatctc
     1021 tgatgtgatg attgctcagg gcctgctgct gcctgcgatt gtggtgctgg ggctgaatat
     1081 ctggaccacc aacgataacg cactctatgc gtcgggttta ggtttcgcca acattaccgg
     1141 tatgtcgagc aaaacccttt cggtaatcaa cggtattatc ggtacggtct gcgcattatg
     1201 gctgtataac aattttgtcg gctggttgac cttcctttcg gcagctattc ctccagtagg
     1261 gggcgtgatc atcgccgact atctgatgaa ccgtcgccgc tatgagcact ttgcgaccac
     1321 gcgtatgatg agtgtcaatt gggtggcgat tctggcggtc gcgctgggga ttgccgcagg
     1381 ccactggtta ccgggaattg ttccggtcaa cgcggtatta ggtggcgcgc tgagctatct
     1441 gatccttaac ccgattttga atcgtaaaac gacagcagca atgacgcatg tggaggttaa
     1501 cagtgtcgaa taacgcttta caaacaatta ttaacgcccg gttaccaggc aaagaggtgc
     1561 tgtggcagat tcatctgcag gacggaaaaa tcagcgccat tgatgcgcaa tccggcgtga
     1621 tgcccataac tgaaaacagc ctggatgccg aacaaggttt agttataccg ccgtttgtgg
     1681 agccgcatat tcacctggac accacgcaaa ccgccggaca accgaactgg aatcagtccg
     1741 gcacgctgtt tgaaggcatt gaacgctggg ccgagcgcaa agcgttatta acccatgacg
     1801 atgtgaaaca acgcgcctgg caaacgctga aatggcagat tgccaacggc attcagcatg
     1861 tgcgtaccca tgtcgatgtt tcggatgcaa cgctaactgc gctgaaagca atgctggaag
     1921 tgaagcagga agtcgcgccg tggattgatc tgcaaattgt cgccttccct caggaaggga
     1981 ttttgtcgta tcccaacggt gaagcgttgc tggaagaggc gttacgctta ggggcagatg
     2041 tagtgggggc gattccgcat tttgaattta cccgtgaata cggcgtggag tcgctgcata
     2101 aaaccttcgc cctggcgcaa aaatacgacc gtctcatcga cgttcactgt gatgagatcg
     2161 atgacgagca gtcgcgcttt gtcgaaaccg ttgctgccct ggcgcaccgt gaaggcatgg
     2221 gcgcgcgagt caccgccagc cacaccacgg caatgcactc ctataacggg gcgtatacct
     2281 cacgcctgtt ccgcttgctg aaaatgtccg gtattaactt tgtcgccaac ccgctggtca
     2341 atattcatct gcaaggacgt ttcgatacgt atccaaaacg tcgcggcatc acgcgcgtta
     2401 aagagatgct ggagtccggc attaacgtct gctttggtca cgatgatgtc ttcgatccgt
     2461 ggtatccgct gggaacggcg aatatgctgc aagtgctgca tatggggctg catgtttgcc
     2521 agttgatggg ctacgggcag attaacgatg gcctgaattt aatcacccac cacagtgcca
     2581 gaacattgaa tttgcaggat tacggcattg ccgccggaaa cagcgcaaac ctgattatcc
     2641 tgccggctga aaatggattt gatgcgctgc gccgtcaggt tccggtacgt tattcggtac
     2701 gtggcggcaa ggtgattgcc agtacacaac cggcacaaac caccgtatat ctggagcagc
     2761 cagaagccat cgattacaaa cggtaatttc ctctgtgttg aagagccact gacctgtgtc
     2821 agtggctacc gtgattcatt ctcgccaaca tccgcacatt cctccaacgc catgtgcaaa
     2881 aatgccttcg cggcggctgt ctgccagcta tttttccgcc gcaacaaaac cgccgttctc
     2941 tccagtagtg gcggggcaag agaaatcgct ttaagcccgt catgttgtgt ggcaatcgct
     3001 gctggtaaca atgtggaaag ggaagtgcgg cgaatcagct ccagaaccgc gctaattgag
     3061 ttcgcctcaa tgaccacctg tggatgtagc cccgctttct cgcagtagtg gtcaatttgc
     3121 tctctggtgg caaattccgc gctgagcagg accagttttt catcatgcaa gcgactcaac
     3181 gccacctgtt cacaggcagc cagcggatga tgttgcgcca cgactaacgc taaactttct
     3241 gtcagtaaag gaattgcctc cagctccggc gaatgcacag gcgcgaaggc aatcccaacg
     3301 tccagctcgt cgcggcaaag cagatcttcg attttctcct gcgacatttc ctgtagctgg
     3361 agcgtgatgc cggggtagcg cgcatagaaa tccgccatta aagggccgat aaagtagctt
     3421 gtaaaggtgg gggtgacggc gatacgcagc gatcctcgcg tcagatcggc aacatcatga
     3481 atcgcccgtt tacccgctcc cagttcctgt aacgcccggc tggcgtactg tcgccagact
     3541 tctcctgcat cggtgagacg aatcgttcgc ccgctacggt caaacagcgg cacgcctaaa
     3601 ctctcctcta actggcgaat ctgctgggaa agcgcaggct gagagacgtg caacgcactg
     3661 gcggcacggg tgaagctgcc atgttcagcc acggcaagaa aataattgat atgtcgagag
     3721 agcattcgcc aacctataag taaatccaat agaactcatc ataaatgaga cttttacctt
     3781 atgacaatcg gcgagtagtc tgcctctcat tccagagaca gacagaggtt aacggtgaaa
     3841 gagattattg atggattcct taaattccag cgcgaggcat ttccgaagcg ggaagccttg
     3901 tttaaacagc tggcgacaca gcaaagcccg cgcacacttt ttatctcctg ctccgacagc
     3961 cgtctggtcc ctgagctggt gacgcaacgt gagcctggcg atctgttcgt tattcgcaac
     4021 gcgggcaata tcgtcccttc ctacgggccg gaacccggtg gcgtttctgc ttcggtggag
     4081 tatgccgtcg ctgcgcttcg ggtatctgac attgtgattt gtggtcattc caactgtggc
     4141 gcgatgaccg ccattgccag ctgtcagtgc atggaccata tgcctgccgt ctcccactgg
     4201 ctgcgttatg ccgattcagc ccgcgtcgtt aatgaggcgc gcccgcattc cgatttaccg
     4261 tcaaaagctg cggcgatggt acgtgaaaac gtcattgctc agttggctaa tttgcaaact
     4321 catccatcgg tgcgcctggc gctcgaagag gggcggatcg ccctgcacgg ctgggtctac
     4381 gacattgaaa gcggcagcat cgcagctttt gacggcgcaa cccgccagtt tgtgccactg
     4441 gccgctaatc ctcgcgtttg tgccataccg ctacgccaac cgaccgcagc gtaaccttat
     4501 ttttaaacca tcaggagatc caccatgatt cagtcacaaa ttaaccgcaa tattcgtctt
     4561 gatcttgccg atgccatttt gctcagcaaa gctaaaaaag atctctcatt tgccgagatt
     4621 gccgacggta ccggtctggc agaagccttc gtaaccgcgg ctttgctggg gcagcaagcg
     4681 cttcctgccg acgccgcccg cctggtcggg gcgaagctgg atctcgacga agacgccatt
     4741 ctactgttgc agatgattcc actgcgtggc tgcattgatg accgtattcc aaccgaccca
     4801 acgatgtatc gtttctatga aatgttgcag gtgtacggca caaccctgaa agcgttggtt
     4861 catgagaagt ttggcgatgg cattatcagc gcgattaact tcaaactcga cgttaagaaa
     4921 gtggcggacc cggaaggtgg cgaacgtgcg gtcattacct tagatggtaa atatctgccg
     4981 accaagccgt tctgacagcc atgcgcaatc atcaaaagac gttcacgatg ctgctggttc
     5041 tggtgctgat tggtcttaat atgcgaccac tgctcacctc cgtcgggcca ctgctaccgc
     5101 aattgcgcca ggcgagcgga atgagcttta gcgtggctgc cctgttgacc gctcttccgg
     5161 tggtcaccat gggcgggctg gcgctggccg gaagctggct tcatcagcat gtcagcgaac
     5221 gtcgcagtgt cgccatcagt ctgttgctga ttgccgtcgg tgcattgatg cgtgagcttt
     5281 acccccaaag tgtgctgctg cttagcagcg cactgcttgg tggggtgggg atcggtatca
     5341 ttcaggcggt gatgccttca gtgattaaac ggcggtttca gcaacgcacg ccactggtga
     5401 tggggctgtg gtctgcggct ctgatgggcg gcggtggact tggtgccgcc ataactccct
     5461 ggttagttca acatagcgaa acctggtatc aaacactcgc ctggtgggcg ctgcctgccg
     5521 ttgttgcgct ctttgcctgg tggtggcaaa gcgcccgcga ggtcgcctct tcccacaaga
     5581 caacaaccac tccggttcgc gtggtattca ctccccgcgc gtggacgctg ggtgtttact
     5641 tcggtctgat taacggcggt tacgccagcc tgattgcctg gttacccgct ttctatattg
     5701 agattggtgc cagcgcgcag tacagcggtt ccttactggc attgatgacg cttgggcaag
     5761 ccgcaggagc tttgctgatg cctgctatgg ctcgccatca ggatcggcgc aaactgttaa
     5821 tgctggcgct ggtgttacaa ctggtggggt tctgcggctt tatctggttg ccgctgcaat
     5881 tgccggtatc gtgggcgatg gtgtgtgggt taggtctggg cggcgcgttt ccgctctgtt
     5941 tgctgctggc gctcgatcac tctgtgcaac cggctattgc tggcaagctg gtggcgttta
     6001 tgcaggggat cggttttatc atcgccgggc ttgccccgtg gttttctggc gtgctgcgta
     6061 gtatcagcgg caattacctg atggactggg cgtttcatgc gctgtgcgtc gttgggctga
     6121 tgatcataac cctgcgtttt gcaccagcac gttttccgca gctgtgggtc aaagaggtat
     6181 gatgcgacgc ttgtttcgcc agatgatgga gctataaata attgtcggat gtgcccagtg
     6241 gcgcatccga cattaattgc ttaagcgact tcattcacct gacgacgcag cagggaaagc
     6301 gggccggggc cgctaagcgt gaacacggaa attaaggtga agcccagcgc caccagaccc
     6361 agcaccagat aagcgccctg gaaaccgatg ctttcataca tattgcccgc cagtacagac
     6421 ataaaaatca tcgccagttg cttaaagaag cagaaacaga ccagataaat cgtcgctgaa
     6481 aaacgcactt caaactggct ggtaatatat ttaaagcagc ccaccagcag gaacggtact
     6541 tcaaacatat gcagcgtttt cagaataacc acttccagcg ctgaggtggc gaacgatgag
     6601 ccaataatac gtacagacat aatagtgcca gccagcagca gggcgttttt cccaccgatg
     6661 cgattaatga tcagtggcgc aaagaacata atcgaggcgt taagtaattc gcccattgtc
     6721 gttacgtagc caaatacccg cgtaccctgt tcaccggtag caaagaacga agtaaagaaa
     6781 ttagcaaact gttggtcaaa aacatcgtag gtgcaggaaa cgccaataac atacagtgac
     6841 aaaaaccaca gttttggctg tctgaacagt tccagtgcca gcttaaggct aaatgccgaa
     6901 tggttggcac ctaccgcatt ggcaaccgtg gcggaagagg gcgcatccgt tttggcgaaa
     6961 aagagtaaaa cggcgaggat gagtgcacag ccagaaccca gccagaaaac aaactgatta
     7021 ttgatggtga acatgatgcc gacaatcgag gcacacaacg cccagccaac acagccaaac
     7081 atccgcgcgc gaccaaattc gaaattactg cgacggctga ctttctcaat aaatgcctct
     7141 actgctggcg caccggcgtt aaaacaaaag cctagataaa taccaccaac aatcgatcct
     7201 actaaaatgt tgtattgtaa cagtggcccg aagataaaaa taaagaacgg cgcaaacatc
     7261 actaacatgc cggtaataat ccacagcagg tatttgcgca gcccgagttt gtcagaaagc
     7321 agaccaaaca gcggttggaa taatagcgag aacagagaaa tagcggcaaa aataataccc
     7381 gtatcacttt tgctgatatg gttgatgtca tgtagccaaa tcgggaaaaa cgggaagtag
     7441 gctccc
//
LOCUS       DAZGLJ010000216         7411 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000216 DAZGLJ010000000
VERSION     DAZGLJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.216"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(53..876)
                     /locus_tag="ACHK49_004060"
                     /pseudo
     CDS             complement(53..876)
                     /locus_tag="ACHK49_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(930..2648)
                     /gene="proS"
                     /locus_tag="ACHK49_004061"
     CDS             complement(930..2648)
                     /gene="proS"
                     /locus_tag="ACHK49_004061"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO9264603.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2760..3467)
                     /gene="tsaA"
                     /locus_tag="ACHK49_004062"
     CDS             complement(2760..3467)
                     /gene="tsaA"
                     /locus_tag="ACHK49_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO9264604.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3464..3868)
                     /gene="rcsF"
                     /locus_tag="ACHK49_004063"
     CDS             complement(3464..3868)
                     /gene="rcsF"
                     /locus_tag="ACHK49_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO9264605.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(3986..4801)
                     /gene="metQ"
                     /locus_tag="ACHK49_004064"
     CDS             complement(3986..4801)
                     /gene="metQ"
                     /locus_tag="ACHK49_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO9264606.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4841..5494)
                     /locus_tag="ACHK49_004065"
     CDS             complement(4841..5494)
                     /locus_tag="ACHK49_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO9264607.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5487..6518)
                     /gene="metN"
                     /locus_tag="ACHK49_004066"
     CDS             complement(5487..6518)
                     /gene="metN"
                     /locus_tag="ACHK49_004066"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO9264608.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6706..7281
                     /gene="gmhB"
                     /locus_tag="ACHK49_004067"
     CDS             6706..7281
                     /gene="gmhB"
                     /locus_tag="ACHK49_004067"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO9264609.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         1813 a         1924 c         1885 g         1789 t
ORIGIN      
        1 cggataaggc gtttacgccg catccggcat tttgtgcacg aggctggctt tatcactgag
       61 tcagcccaac aaagcgaccc gccttgatat aaatcccagg cttcaggtgc ccaacatact
      121 gtaactgcgt ttctggctta aacgcagaga catcaccaat acgcatatgc atcagatcgg
      181 cagggctaat ccagcccgat tgcgccagtg taagtggatg cccggctttg gcaaaggcat
      241 ccgtgacgaa ttccgaacaa aaccacgact ttttgtctcc ttcgcccaca ctgcttaact
      301 gcgctttcgc caggccgctg acgcactttg gcgaaaatcc tcggagaacg gattcagtga
      361 gcacatctgg cgagtcacca taaagggaat aaattcgaca atgccgcgat agttataacc
      421 gctatctttg attttattgg caaacgcggt gatatctgtg gcttgttgcg gggtaagatc
      481 cgggactcgt aagacgaaaa gcttatcact atgcttcatc gcttttttaa gggaaacaat
      541 ctggacgcca gcgcctgtcg cttctgcaac gttattatca cccagaaaga ttgcaacgtg
      601 actcacagag gaagtgctga agacgcggat tccaaatgag gttaccccaa ggcttgagga
      661 gaacagcaaa tcgccgggtt tgagatcagg tgccgttatt tcttttattg attgttcggt
      721 gaaagagctt tgatgctgga atttgacagc ccatgttttt gcctcggcat ctactgctgt
      781 ggctgatggg tctggctggc tgatatcaac ggtacaagcc gaaagtaaaa gaaagctggg
      841 gagaaacagg cggcagtacg cctttggttt atccatttta tacaatccat gtaaaaaaag
      901 ggccctgaaa ttcaggaccc tttctggcat cagcctttaa tctgtttcac cagatattcg
      961 acgatgtcac cagtcttaat taactgtttc tcgccgttgc gacgatattt atattcgata
     1021 tcgtcgttgt cgaggttacg gtcgcccagc acaatagtgt gcggaatacc gatcagttcc
     1081 atatcagcaa acatcacgcc cggacgctct ttgcggtcat ccagcagcac ttcgatacct
     1141 tgtgcacgca gttcgctgta cagtttctca gcaagctctt gtacgcggaa ggatttgtgc
     1201 atgttcatcg gcagaatcgc cacctggaac ggcgcgatag cgtcaggcca tacgatgcct
     1261 cgttcgtcgt agttctgctc aatcgccgca gctaccacac gcgttacccc gataccgtag
     1321 caacccatcg tcaggatttg gttacggcca tcttcaccct gtacggaggc tttcagtgct
     1381 tcggagtact tggtacccag ctggaagatg tgaccaactt cgataccacg tttgatcagc
     1441 agcgtaccct ggccatccgg gcttggatcg ccagccacca cgttacggat atctgcaact
     1501 tccggggtag cgacatcgcg atcccagttg atgccgaagt agtgtttacc atcgatgtta
     1561 gcaccagcag cgaaatcact catcgccgca acggtacggt caatcaccac cggaatcggc
     1621 atgtttaccg gacccagtga acccggaccg gctttaacca cggcacgaat ttcttcttcg
     1681 gtcgcgaaag tcagcgggct tgcaacctgc ggcagttttt ctgctttaac ttcgttcagc
     1741 tcgtgatcac cgcgcaccag cagcgcaacc agcgggaagc tactgccttc aaccgcttta
     1801 accagcagag tcttaaccgt tttctcaatc ggcagattga actgttcaac cagttccgcg
     1861 atggttttcg cgttcggcgt atcaaccagc gtcatttcct gggtagcagc agcgcgcggt
     1921 tctttcggcg cgatagcttc tgccagttca atgttcgctg catagtcaga ggtgtcggag
     1981 aagaccacat cgtcttcacc gctctgcgcc agcacctgga attcgtgaga ggcgctgccg
     2041 ccgatagaac cggtgtcggc ttgtacggcg cggaaatcca gccccatgcg gctgaagatt
     2101 ttgctgtagg ccgcatacat tgcatcgtag gtttcctgca gggattcctg agaagtatgg
     2161 aaagagtaag catctttcat caggaattcg cgggaacgca tgacgccgaa acgcggacgc
     2221 acttcgtcgc ggaacttggt ctggatctga tagaagttca gcggcagctg tttgtaagag
     2281 ctaagctcgt tacgaatcag gtcagtgata acttcttcat gagttgggcc gagtacgaac
     2341 ggacgctcgc cacggtcaac aaaacgcagc agttccggac cgtactgttc ccaacgacca
     2401 ctctcttgcc acaaatcagc tggctgaacc accggcatcg acacctcgat cgcaccggcg
     2461 ttgttcatct cttcacgcac gatgttttcg acttttttca gaacgcgcac gccggtcggc
     2521 agccaggtat ataacccgga ggccagcttg cggatcatcc cggcgcgcag catcagctga
     2581 tggctgatca cctcggcgtc ggcaggtgtc tccttgagag tggagagcag gtattggcta
     2641 gtacgcatgt tgttacggtt ccagttggaa ggtagaacag gctcaaggcg agcctgggac
     2701 aaaaaaaagt gatttagttt accagtgcaa aagaaatgtc aaaagagaag ggcgtgaatt
     2761 taacgcggtt ccagcgcaaa gacttcaaaa cctgcgtcgg tgacgcgcca gcgaacgtta
     2821 aaatcatgta gccagacggc ataggttttg cccgtttcct cacctttacg ataggccggg
     2881 cgtgggtcct gcgccagtac ttcgcggata aacagcgtta actgcggata acgcttctcc
     2941 agcgtcaaaa gctgcttttc gacctctgcg gtaaaactca ccgccatctc tgccgctggc
     3001 gcactttgcg catagctggc actggcatcg ggaagcgatt cggcaaaggg gagatacggt
     3061 ttgatatcca ctaccggcgt accatcgacc agatccagac tgccgagctt cagaatcacg
     3121 ctgtctttat ggcaaacaac ctctttcagc tctaccagcg acataccaat tgggttaggg
     3181 cggaaagtag agcgtgttgc gaaaaccccc attctggcgt taccgccgag gcgcggtgga
     3241 cgcacagtcg gacgccagcc gccttccatc gtttgatgaa agacgaaaag gatccataaa
     3301 tggctgaacg cttccaggcc gcgaacggcg tcggcctggt tgtagggagc aatgagatgc
     3361 agttcgccgt tggcgctttt taccagacct ggctggcgcg gaacggcgaa tttttcttta
     3421 tagggcgagc gaataacgcc tatttgctcg aactggaaac tgctcatttc gccgtaatgt
     3481 taagcgcaga gccgatacat acagcctgac gatagcagcc tggcgtaccg ctggtgactt
     3541 cgcagctatg cagtaatacc gcattggcct tcattttcga ggcgttgatt tgcatccgct
     3601 tacgcgcggt tggaatgctc ggcggagagt cctgattaga ggcctggcaa gagtcgccac
     3661 tgacttcacc gagatcgcgg aacggtttgc cgactaattc ttctgcattg gtataaattc
     3721 ggaccggcgt ggcgcgcggc gctttcggtt ttgcaggctc cgctttcggc tggggtgcag
     3781 tgctttgaac gggttcgaca ggggatctgc ttaacatgga acagccgctt agcatgagtg
     3841 ctactaaaca gatcggtaaa gcacgcatag tatttcctca atgtatgatc aaaacgtcaa
     3901 tattgaatca ggagcttgta aaaatgacaa gacgggcaag cgcccgtcct gaatgatatt
     3961 acaaattgtg gaaacagcct aaaaattacc agcctttaac agcgccgccg ttaaacactt
     4021 tgtttgctgc ttcgtaaact tcatcagact gataagcctg gacgaatttc ttcacgttct
     4081 cggcgtcttt gttatcttca cgcgttacga tcaggtttac gtacggggag tctttatctt
     4141 cgacaaagat accgtctttc gctggagtca ggccaatctg gctggcatag gtggtattga
     4201 taactgccag agcgatttgc gcgtcgtcca gagatcgagg cagctgcggt gcttccagct
     4261 caacaatttt cagatttttt gggttctcaa caacatcaag aacggtcggc agcaggccaa
     4321 cgccatcttt cagtttgatc aagcccactt tttgcagcag cagcagtgaa cgaccaagat
     4381 tagttggatc gtttggcacg gcaacctgcg aaccatcctg cagttcatcc agtgatttga
     4441 ttttcttgga gtaaccagca atcggataaa caaaagtgtt gcctactgcg accagtttgt
     4501 agccacgatc tttcagttgc tgatcaaggt acggtttatg ctggaaggcg ttggcgtcga
     4561 tatcgccttt gctcaatgct tcgtttggca gaacatagtc gttgaaggtt accagctcaa
     4621 cgtccaggcc atatttgtct ttcgcaactt tctgcgcgac ttctgctacc tgctgttcgg
     4681 caccaacaat cacgccgact ttaatgtggt ttggatcttt ttcatcctga ccgcagccta
     4741 ccagtgccag tgatccaatc agggctccca ctgccgcaaa ggttttgaat ttgaacgcca
     4801 taccttattc cttcttcaat tatttatgtt gtgttgaacg ttacttgcga gtgacagccc
     4861 ggacgatgcg gtcgcctgcg aactgaatta aataaaccag aatgaccagc aataccagta
     4921 ccgtattcat caccgtcgcg ttatagccga tgtagccata ctgatagcca atctgaccta
     4981 aaccaccggc accgactgca ccacccatcg cggaataacc gaccagggta atcagggtga
     5041 tagttgccgc attcaccaga cccggcaatg cttccggtaa cagcacttta cggacgatct
     5101 gcatcggcgt ggcacccatt gcacgggaag cttcaattaa cccggttggg atctccagca
     5161 gagcgttctc gaccatacgg gcaataaacg gtgctgcacc aacggttaac ggaacaatcg
     5221 ctgcctgcaa accaatcgat gtaccgacaa taacgcgggt aaacggaatc atccatacca
     5281 gcaagataat gaacgggatg gaacggaaaa tgttcacaat cgcagaaacg gtacgataca
     5341 gcttcgcgtt agcaataatt tgccccgggc gcgtaacata aagcaggacg ccgaccggca
     5401 gaccaatcac aaagccaaaa aatccggata cgaaggtcat tgccagcgtt tcccatacgc
     5461 cacgaaccag caaccacatc atcggctcag acataaccca gtacctctac ttttacatgg
     5521 tgttcctgca gccaggcaat ggcggcttgc gtatcttgtt gtgtgccgtg catttcagtc
     5581 agcatgatgc cgaacttaac gccaccggcg taatccatct gcgcgctaat aatgttgttg
     5641 ttgacgttga aacgacgcgc ggtttcagaa agcagcgggg catcgaccga ttgaccggta
     5701 aactccaggc gcaacatcgg cacgcagtca gtaaatggct ccgcttgcag acgttcctgg
     5761 taatcttccg ggatatccag atgcagggtc gactgaataa acttctgcgc cagcggcgtt
     5821 ttcggatgcg agaacacttc acttaccgtg tcctgctcga tcagttcgcc attgctgatg
     5881 accgccacgc aatcacaaat acgcttcaca acgtccattt cgtgggtgat caacagaatc
     5941 gtcaaaccca gacggcggtt aatgtctttc agcagttcga gaatagaacg tgtcgttgcc
     6001 gggtccagcg cgctggtggc ttcatcacac agcaatactt tgggattgct ggctaatgca
     6061 cgggcaatcg ccacacgttg tttctgccca ccggaaagat tcgacgggta gctatcatgc
     6121 ttatcgccaa gaccaaccaa tgacagcaat tccgtcacgc gacgtttgat ctcgtctttc
     6181 ggtgtgttgt ccagctccag cggcagagcc acgttgccaa aaacagtacg cgaagagagc
     6241 aggttaaaat gctggaaaat cataccaatc tggcggcgag ctttggtcaa ctcggattct
     6301 gacagcgtgg tcagttcctg gccatcgacc agcacgctac cctcggttgg gcgctccagc
     6361 aggtttacac aacgtataag cgtactctta cccgcgcctg aggcaccgat aacgccataa
     6421 atttgtccag ctggcacatg caggctgacg ttgttcaacg cctggatggt gcgggtgccc
     6481 tggtggaaca ctttggtgat attcgaaagt tttatcattg attatttatt atcgtcatta
     6541 agttagtcgt ggcatctcga atgcctgaaa cgggcaacgc cgtcaatgaa atggatgtta
     6601 aggcatccag acgtctaaat caatcaggtt tatgcgaaga gcactttctt gcaggtcgaa
     6661 acatgcgata ctagcgtccc atgccttatt aaggagctat aaaaggtggc gaagagcgta
     6721 cccgcaattt ttcttgaccg tgatggcacc attaatgtcg atcacggcta tgtccatgag
     6781 atcgacaact ttgaatttat cgacggtgtt attgacgcca tgcgcgagct aaaaaaaatg
     6841 ggctttgcgc tggtggtagt aaccaaccag tctggcattg ctcgcggtaa atttaccgaa
     6901 gcacagtttg aaacgctgac cgagtggatg gactggtcgc tggcggaccg agatgtcgat
     6961 ctggatggta tctattattg cccgcatcat ccgcagggta gtgttgaaga gtttcgccag
     7021 gtctgcgatt gccgcaaacc acatccgggg atgcttttgt cagcacgcga ttatttgcat
     7081 attgatatgg ccgcttctta tatggtgggc gataaattag aagatatgca ggcagcggtt
     7141 gcggcgaacg tgggaacaaa agtgctggtg cgtacgggta aacctattac acctgaagca
     7201 gaaaacgcgg cagattgggt gttaaatagc ctggcagacc tgccgcaagc gataaaaaag
     7261 cagcaaaaac cggcacaatg attaaaagat gagcggttga aataaaaatg catttttccg
     7321 cttgtcttcc tgagccgact ccctataatg cgcctccatc gacacggcgg atgtgaatca
     7381 cttcacacaa acagccggtt cggttgaaga g
//
LOCUS       DAZGLJ010000217         7369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000217 DAZGLJ010000000
VERSION     DAZGLJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.217"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            458..691
                     /locus_tag="ACHK49_004068"
     CDS             458..691
                     /locus_tag="ACHK49_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264610.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
     gene            complement(669..1076)
                     /locus_tag="ACHK49_004069"
     CDS             complement(669..1076)
                     /locus_tag="ACHK49_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264611.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(1099..1317)
                     /gene="ydfC"
                     /locus_tag="ACHK49_004070"
     CDS             complement(1099..1317)
                     /gene="ydfC"
                     /locus_tag="ACHK49_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO9264612.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(1390..1689)
                     /locus_tag="ACHK49_004071"
     CDS             complement(1390..1689)
                     /locus_tag="ACHK49_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264613.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            complement(1954..2361)
                     /locus_tag="ACHK49_004072"
     CDS             complement(1954..2361)
                     /locus_tag="ACHK49_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9264614.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            2438..2665
                     /locus_tag="ACHK49_004073"
     CDS             2438..2665
                     /locus_tag="ACHK49_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO9264615.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            2649..3200
                     /locus_tag="ACHK49_004074"
     CDS             2649..3200
                     /locus_tag="ACHK49_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO9264616.1"
                     /translation="MKITTEQVCEALDTCVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGSYEYRCTTMG"
     gene            3172..4212
                     /locus_tag="ACHK49_004075"
     CDS             3172..4212
                     /locus_tag="ACHK49_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO9264617.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            4205..4666
                     /locus_tag="ACHK49_004076"
     CDS             4205..4666
                     /locus_tag="ACHK49_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO9264618.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            4700..5433
                     /locus_tag="ACHK49_004077"
                     /pseudo
     CDS             4700..5433
                     /locus_tag="ACHK49_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            5430..5594
                     /locus_tag="ACHK49_004078"
     CDS             5430..5594
                     /locus_tag="ACHK49_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264619.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            5599..6132
                     /locus_tag="ACHK49_004079"
                     /pseudo
     CDS             5599..6132
                     /locus_tag="ACHK49_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            6293..7051
                     /gene="paa"
                     /locus_tag="ACHK49_004080"
     CDS             6293..7051
                     /gene="paa"
                     /locus_tag="ACHK49_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HFO9264620.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTG
                     IIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSIL
                     LVKKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRK
                     NIILYAPNSGTARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITV
                     RQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
BASE COUNT         2034 a         1604 c         1886 g         1845 t
ORIGIN      
        1 actgtttttg gttttaactg ttccgcgtgc gctcagccgc attcaccaca tcacaaaatt
       61 cactttaaaa agggcggcag agcagtcacg gagtaaaact gataccgcca aacgtcacca
      121 gaaaattgat aacagagggc gttgcagcgg ggttgtcact taagcgtatg gtcaacctga
      181 caacccggtg tcctcaacgg ggaaggaata acccctccat acttaccgcc gcgccatttc
      241 gcggattgcc acaaccggaa gcgcacggtc gacgaaaatt taacgacagg ctatctatga
      301 accagctacc tcgccgtgcg ctttcgcgtt atggtctgac ttttcagaga aatatccttt
      361 cagtaaactg tcagtgccgg atgttcaccc gtgtccggcg cacgcactcc acttcacccg
      421 tggagaactc cttaattacc aaccctcagg agggtgaatg ttaaaatcaa ctcttattgc
      481 taaatgcctt tatcaaaatc gcatggtaag cagcatttca ataggcgagt ctgcagttaa
      541 aagtattttc gaagagtact ttcccgggca tgattttaat aaatggaata ccaaattacc
      601 gccagcagtt tcaacgcgta ttctgaaagc aaccgaaaga gcaagtacaa ttcgcgttaa
      661 ctatttcatt aaagatttgt gggatctttg atatccacag agcctaaagt atgtgcatat
      721 ggatgtgcta ttatgcgccc tcgcagattt gcatcatttt ctaaattcac tgaacgaaac
      781 agggcatcaa caaggctctg tacaatgcaa aggcaatcga agactgtcgc cgtttctgtt
      841 ttgattgatg aaagaacatg gccattcacg caaacagaaa ttacccgttt attaacatcg
      901 ctttcctgct tttgattatc agaaccatat agcccagaaa aagcattgcg cacattacga
      961 accatattat cgatggtttc tttttctgcg gtactaaggt caagagtagc cagttgtgaa
     1021 cgaactatat tcgatgccat ttcctgtaat ggcgttggta aatctttaaa ttccattatt
     1081 agcctcgttg gttagctatt aacgtgggta tgtaatcatt ctggcaatgc ttaatgccgc
     1141 tgctttttcc agattggtga tatcctgctc cagagcggac agattttcag cctgcttagc
     1201 cctggcttca ttggcccatt tcaggtcctg caccgcctta attttctggt gcatccactc
     1261 ataaagttca tcatcggtat agtctggcgc gatgatgacg gggtctcgtt tctgcatgtc
     1321 ggctccttgt ggttagcgtt gcctgctttt taaccacgtc aggcgaggtg gtatcctctg
     1381 aggggtctgt tactcgagag gaaattggtt atgaatacaa tcaagttttc ttgcccagaa
     1441 tgtggtggct aagtctttga cacatccttt aagccgcagg gctctgacag tttcgcggga
     1501 gccatctgca aaaattgtgg tcaccttgta actgaagatg agtcctcgca gttcgatgac
     1561 gaaatcgttg acaatatctt cggtgcactc accagagact ttctgaagta aaggcgcata
     1621 ccgcttagtt accgctctga taactcttac ctgtccggca atggcgctga tatcaatata
     1681 aagcgccatc gctgtttctt tgctgatccc tggacgcctt ccattctgat gtttgacttc
     1741 gcccactgag aaatcctctg cttcccctta acgccgggta gcggaactgt ttgctgagaa
     1801 caccgtgcgg tgtcttgatg agtagaattt agaatagcct aagagttatg gtcaagcttt
     1861 ttgtgtagaa aaacctaagc ttcttgatgt aaaaaacaca agtatttgaa agtttgtgct
     1921 ttttattaca gagagttgcg aaaaaaaggg gggttattta tttgcgcttc ttttgcgagc
     1981 tttgagtagt tcttcaaaaa gtttgttgaa attctcaact cgagcacgca tctctgacaa
     2041 cagagccttt tgctctgact caggcagtgc gtcgaacagt tgaagcaact ctttttgatc
     2101 ttctgtcaga ttgactggct gattatctgg gatcggttcg cctggttgct tatcttcatc
     2161 tccaaaaaga agccaagtcg gcgagcactg aagcgcctgg ctcagtgcga ataatctctt
     2221 ccccgctggc tgtgtttcat ctctttccca ttgagaaatt gttacgtgag ccactttgac
     2281 cagcttacct aatgcggcct gagacagttt taattttttt cgcctgtata agaggcgagc
     2341 accgaaggtt tcgtttttca tattaggtaa ttctaatttt tcttgactta ggtttctcta
     2401 cgatctagtt tccttaggaa aatctaaggg gttcgatatg ttgaaaattg atgctatagc
     2461 gttttttggc agcaaaacaa agcttgccaa tgtcgcagga gttaggctgg caagcgttgc
     2521 tgcatggggg gaactggttc ctgaaggtcg cgcgatgcgc ctgcaagagg catccggcgg
     2581 ggaacttcag tacgacccca aagtttatga cgaatatcgt aaggcaaagc gggcggggcg
     2641 gttgaacaat gaaaatcacc actgaacagg tttgtgaggc tctggatacc tgtgtatgcc
     2701 gaccaggaat gacacaggag caggcgacga tattaatcac ggaagcattc tgggctctga
     2761 aagaacgccc gaacatcgat gttcaacgcg tcacgtttaa tgatggcgag gttgatcaac
     2821 gggcgctggg cgttaaccgg gtgaagatat tcgaacgctg gaaagctatc gacaccagag
     2881 ataagcgtga caaattcacg gcgctgattc cggcaattat ggaggctatc cggatcagcg
     2941 atttcagatt gtattgtgaa attactgacg gaaaaagcat tacgtacatg atcgccgggt
     3001 taaacaaaga atatggcgat gtggtggagt ccgggctgct ttttgcggat ccagttgttg
     3061 tggaacgtga gactgacgag cttatagaaa aagctattgc tttcaagcac gcgtatcgtc
     3121 agcaatacca atattacttt gcagataaac aaatgtctgc caggggttcg tatgagtatc
     3181 gatgcactac gatgggctaa aaaggtgaaa accggcagtt catccagtaa gtcagtattg
     3241 acctggcttg ctgatatgtg cggtgccgat ttgtgtgcat acccgtctgt atctgcactg
     3301 gcagaagtaa cggaactgaa caaaaagact gtgcaggaca gcttacgaca cctgatggag
     3361 attgggttaa ttgttgatac cggtgagaga aaaggcagaa caaagcaaat tgtggtgtac
     3421 cgacttatcg gtgtagaaga aagtgttgcc gagcctgaat acacccaaaa acgggagtct
     3481 ttaaaggtgg gtaaaattgg tgctgttaat aaaaacagta ccgaaaatgg ttatgtttca
     3541 gcacaaaaca gacccaaaaa cagaactctt agctgcatgg aaaataacca aagacaccca
     3601 aattttccat caaagacacc caaaaacgga tcacggaacc caaaggaacc caaagatcta
     3661 aaccccacac ataacgcacg cgagagtgct ccgaccagtg agcaggaagt tttgtcgtta
     3721 caggcagccc cccctgtatt cctgtatggc ctgagcgaac ccatcggaaa attcccgatg
     3781 agcgatagct ggtatccgtc acgggatttt cgacgacggg ctgcgttgtg ggggatggct
     3841 ttgccggaga cagaatttac acctgctgaa cttgccgcct tccgggacta ctgggcagcg
     3901 gaggggaaag tgtttacgca gattcagtgg gagcagaaat tcgcccgtca cgtaaatcac
     3961 gtcagggcgc aggttaaacc agtcagcaaa ggggtaaacc atgcagcagc accaggtggc
     4021 accgcatcac gggcagttca ggaaattcgg gcagcacgtg agcagtggga acgtgaaaac
     4081 ggatttatca gcgacggaaa cggcctggaa gctgtgggaa ctcatggggg tggtttattc
     4141 gaaccgctgg atccagaaga acggggccgc accttcgaag ctctggattg cacagattgg
     4201 cgcgatgact gagcagcaaa tccggcaggt ctgccgccag tgcatggacc gctgccgggc
     4261 gggtgaaaca tggcctccgg acctggctga gtttgtggca ctgatttcga aaagcggagc
     4321 caatccattc ggtctgacgg tggatgctgt gatggaggag taccgccgct ggcgcaatga
     4381 gtcctggcga tacgacggaa gtgataagta cccgtggtct cagcctgtgc tgtatcacat
     4441 ttgcctcgag atgcgttcaa aggggattga gcgccagatg accgaagggg aattaaaacg
     4501 gcttgcagaa cggcaactga cgaaatgggc aaagcatgtt agtaacggcc tgagcgttcc
     4561 gccagtccgg cgacaactgg cggcccccaa acgcccgtcg gggccaacgc caattgagtt
     4621 gctgaaacag gaatatgaac gccggaaagc ggctggtttt gtttgagttg agaagtgatt
     4681 ttttaccggg aggaaattta tggagactgt ttttgacgca ctgaaagcga tgggaaaagc
     4741 cacgtcggta gagctggctg cgcgacttga tatcagtcgt gaagaagtac tgaacgagct
     4801 gtgggaactg aaaaaggctg gcttcgttga taaaagcgta tacacctggc gtgtggctga
     4861 taacaacgtt cagcaggaac agccagcgcc agaagaaacc accacggcaa cagaagcgaa
     4921 aatctcagag tgcgatttaa ccgcgacgat tgaacaacgc ggaccacaaa cggcggatga
     4981 actggctacg ctgttcggta caacatcccg caaagttgct tcaacgctgg caatggcaat
     5041 cagcaaaggt cgtctgattc gcgtaaacca gaacggtaaa attcgttact gcataccagg
     5101 tgataattta ccagcagagc cgaaagttga atcggtagcg gaaaccgatg gtaaagcctt
     5161 tcctcagcca gccggtgttc gttaccggta cagaaagatg caacacagga agatattaaa
     5221 acagaaactg tggcggacat tgtgcagtcg ctgccatcgt ttactgcaac gcgagaagat
     5281 gatttgattt tgccatcgct gcatatggca aatcgcgaac tgcgtagggc gaagaatcat
     5341 gtccagaagt gggagcgagt ctgcgccgcg ctgcgggagc tgaacaagca ccgggatatg
     5401 gttgccggga tttgtcggaa gtccgggcaa tgagcggatg gtgcaggcct gaaatcatga
     5461 tactaacaat gaaggtaaaa tgcatcggca gtctgattgg tcgtagtgag gcggcggtca
     5521 ggatgaaagc ccaggttaag ggaataagcc tgattctgcg gggtgatttt caccagtcaa
     5581 caaaatatcc gtagcgcgat aacggtcaaa aattatggcg ctgacacttt tgtgccactg
     5641 gagatgactg tacctaagtt caggggagaa gaacacgtcc ggtgggatgg tcgggccaga
     5701 tttaaagggc aggtcatggc tccagcctgt acgctggcaa tggaggctgc ctggcgggaa
     5761 attgatatgg gaaccacgcc actcagggat ttactgccgg tccagagaat aaattcctgt
     5821 tacggttaca ccactgtgat cttgcaagtg caggaaagta ggtctacacg gtaacgcgag
     5881 tgcgtgtaac ttttgatgtc attcccgtag aaacaccgga caaattttcg ctgacaggtc
     5941 atgcagaagg tataaatctg cagattatgg acaattacgg atatccggca agagccggaa
     6001 aaagcatgcc gcctctaatt ctcagtggaa gatggacttg attatactca ttgcattgtc
     6061 agaaatagtt atccattaaa ggctggctat tccaaacagg atgttgatta caaaaatgta
     6121 atcaacatgt aaggtttata ctcttcaata tgcgtataat tttccttatt ttgttgactt
     6181 taaataacaa gctatgcacg aggtaaagtc ggataagttt atctggatgt aatatatatt
     6241 atttgtagtg tttataactt tatttcatga taaccaataa aaggagtttt ttatgaggaa
     6301 cataatggca ggttttttaa tattcctgtc ttctgctgct tatgctgata tcaatctgta
     6361 tggtcctggt ggcccgcata cagccttgct tgatgcagcc aaactttatg ccgaaaaaac
     6421 aggtattata gtgaacgttc attacggccc acagaacaaa tggaatgaag atgccaaaaa
     6481 aaatgcagat atcttgtttg gcgcatcaga acaatctgct ctggctatca ttcgggacca
     6541 taaagacagc ttcagtgaaa aagatattca gcctctttat ctgcgaaaaa gtattttact
     6601 ggtaaagaaa ggtaatccta aaaatatccg gagtattgac gacctgacca gacctgggat
     6661 tggcgtaatt gttaatgatg gtggtggtac cagtaataca tcaggcactg gcgtctggga
     6721 agatattgcc ggacgtaaag ggaatataga aactgtcgcc gcaatccgaa aaaatattat
     6781 tttatatgcg cccaatagcg gaactgcacg taaggctctt gagaatcagc ctggagcaga
     6841 tgtctggata acctgggctg actgggcagc cagtaatcca gaaattggtg atgtcgtgga
     6901 aatagcgcca gactacgtga tatggcgtga tatgaacatt acagtacgtc aggatgcaaa
     6961 tgatgaaacc cgtcgatttg cagaatggct acaaaccgat gaagcggcgc ctgcattcaa
     7021 aaaatatggc tggaccagga aaggcacttg acatcctcgt ccttcaggac gtggattctt
     7081 tttccggatg ccgcgccagc ggcatgtagg ggcagctcac aaaacggaaa aaattgtacg
     7141 ctaagcctcg ccaggtgaac tgaattcatt ccgatatggg aattcccata tcgggcgaaa
     7201 acggtttgct gtaacggcag agttaagtag gattgctgcg ggtgcttgag gctatctgcc
     7261 tcgggcatga acaccaacgg cagatagata aaagccccac ccgactataa atcgaagtga
     7321 ggcccctata tgctcgtcac atatagattg cctcttacgg accgaaagg
//
LOCUS       DAZGLJ010000218         7340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000218 DAZGLJ010000000
VERSION     DAZGLJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.218"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            54..314
                     /gene="dinJ"
                     /locus_tag="ACHK49_004081"
     CDS             54..314
                     /gene="dinJ"
                     /locus_tag="ACHK49_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO9264621.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            302..589
                     /gene="yafQ"
                     /locus_tag="ACHK49_004082"
     CDS             302..589
                     /gene="yafQ"
                     /locus_tag="ACHK49_004082"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFO9264622.1"
                     /translation="MRNLNQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNAL
                     PLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            745..1485
                     /gene="dpaA"
                     /locus_tag="ACHK49_004083"
     CDS             745..1485
                     /gene="dpaA"
                     /locus_tag="ACHK49_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO9264623.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1456..2223)
                     /gene="yafJ"
                     /locus_tag="ACHK49_004084"
     CDS             complement(1456..2223)
                     /gene="yafJ"
                     /locus_tag="ACHK49_004084"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO9264624.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2429..3007)
                     /gene="lpcA"
                     /locus_tag="ACHK49_004085"
     CDS             complement(2429..3007)
                     /gene="lpcA"
                     /locus_tag="ACHK49_004085"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO9264625.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            3247..5691
                     /gene="fadE"
                     /locus_tag="ACHK49_004086"
     CDS             3247..5691
                     /gene="fadE"
                     /locus_tag="ACHK49_004086"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO9264626.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTASLGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5734..6180)
                     /gene="ivy"
                     /locus_tag="ACHK49_004087"
     CDS             complement(5734..6180)
                     /gene="ivy"
                     /locus_tag="ACHK49_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO9264627.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6361..7128
                     /gene="yafV"
                     /locus_tag="ACHK49_004088"
     CDS             6361..7128
                     /gene="yafV"
                     /locus_tag="ACHK49_004088"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO9264628.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRL"
     gene            complement(7249..>7340)
                     /locus_tag="ACHK49_004089"
     CDS             complement(7249..>7340)
                     /locus_tag="ACHK49_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264629.1"
                     /translation="LNTCYIEEVLASCSELGFRTDKMKNLTQI"
BASE COUNT         1753 a         1836 c         1963 g         1788 t
ORIGIN      
        1 tcatttgagc tacaattcaa gctgaataaa tatacagcac aggagatacc ccaatggctg
       61 ctaacgcatt tgttcgcgcc cgaatcgatg aagatctgaa gaatcaggcg gcggacgtac
      121 tggccgggat ggggctgacc atctctgacc tggttcgcat aaccctcaca aaggtcgcgc
      181 gtgaaaaggc attgccgttt gatttacgcg agcctaatca attaaccatt caatcaatca
      241 aaaacagcga agctggcgtt gatgttcata aggccaaaga cgccgatgat ttatttgata
      301 aattaggaat ttaaatcaaa gggatattga atactcggga caattttcaa aggatgtaaa
      361 acttgcacaa aagcgtcata aggatatgaa taaattgaaa tatcttatga cgcttcttat
      421 caataatgct ttaccgcttc cagctgttta taaagaccac ccgctgcaag gttcatggaa
      481 aggttatcgc gatgctcatg tcgaaccgga ctggatcctg atttacaaac ttaccgataa
      541 acttttacga tttgagagaa ctggaactca cgcggcgctc tttgggtaaa gcacgctgcc
      601 gatgaaattt gtaaaccctg cgccgacagg cttgcgtaaa actcttttgg tgataatgtg
      661 agccttccaa atccagacta ttccgatttg ggatctattt tgccgtcctg gcgtgtgtaa
      721 ccgttttatc aaggaataag cagtatgcgt aaaatcgcat taattcttgc gatgttgttg
      781 atcccgtgcg tttcgtttgc cggtttgctg ggcagcagta gctcgaccac gccagtgagc
      841 aaagagtata agcagcagtt gatgggatcc cctgtctaca tccagatctt caaggaagaa
      901 cgtacgctcg atctctacgt caaaatgggc gagcaatatc agctgctcga cagttataaa
      961 atctgtaaat attccggcgg cttagggcca aaacagcgtc agggcgattt caaaagcccg
     1021 gaagggtttt atagcgtcca gcgtaatcag ttaaaaccag acagccgtta ctacaaagcg
     1081 attaatattg gtttccccaa tgcctatgac cgtgcgcatg gttatgaagg gaaatacctg
     1141 atgatccacg gcgattgtgt ttccatcggc tgctacgcaa tgaccaatca gggtattgat
     1201 gagatcttcc agttcgttac tggtgcgctg gtgtttggtc agccgagcgt gcaagtgagt
     1261 atttacccgt tccgcatgac cgacgccaat atgaagcgcc ataaatattc caactttaag
     1321 gacttctggg agcagctgaa gccgggctac gactactttg agcaaacccg taagccaccg
     1381 accgtttctg tggtcaatgg ccgttacgtg gtcagcaagc cgttaagcca cgaagttgtg
     1441 cagccacaac tggcatcaaa ctacacgctc cccgaggcaa aataagcgcc attcgcctgg
     1501 cataatcttt tgccaggttt cattgcccgt cagcggctgt gttgcaatca ccgtgaccac
     1561 atcattcggt gtggtctgcg agctgaagtc gatttccaca tcctgatcca gcagcgttgc
     1621 cacgccaaac ggcgcgcggc gggtgatcca gtgtaaatta gtcgagcaat acgccatcac
     1681 atagcgcccg tcagaaagca gcatgttgaa aacgcccttc tgccgcagtt catccgccag
     1741 tgaggcgata tatttaaaca ctgccgccat gttgcccggt gtgcgcgggt aacgctgcgt
     1801 taatttatgc aggagccagc agaaggcttt ttcgctatcg gtttcaccga ccgggcggaa
     1861 gttgccggtt tccagtgatt tgtagcccgt cagttgtccg ttatgggcat aagtccagtt
     1921 acgcccccat aactcgcggg taaatgggtg agtattttcc agcgccacct cgccccgatt
     1981 agcctggcga atatgagcca ccaccgaaca ggattttatc gggtagtcct ggacaagttt
     2041 ggcgatgggg gaattaaagc tgggttgtgg atctttaaat gtgcgacagc ctttaccttc
     2101 gtaaaaggta atgccccagc catctttatg tggcccggtt ccaccaccac gctgtacaag
     2161 tccggtgaaa ctaaagcaga tatcggtagg gacgttggcg ctcatcccga gcaattcgca
     2221 catgggtaac ctccacaaca gcgggagcga atggcccccg gcgaagattg atttaagaat
     2281 tttcaggtcg gatgcggcgt aaacgaatta ttcggcctac ggttcgggta cagatttgta
     2341 ggcatgataa gacgcgtcag catcgcatca ggcatcagcg cacaagtgcc ggatgcggcg
     2401 taaacgcctt atccagccta cgccagactt acttaaccat ctctttttca attaactgga
     2461 tcaggatatg gatcacttta atgtgaatct cctgaatgcg gtcggcataa ccaaagtgcg
     2521 gtacgcgaat ttcgatatcc gccgtgccag ccattttgcc gccgtcttta ccggtcaggg
     2581 tgatcacttt catccccttc tcacgcgccg ctgcgatcgc tttgatcacg tttgcagagt
     2641 taccggaggt ggagatcccc agcagtacat cgccttcgcg acctaccgct tcaacgtagc
     2701 gggagaaaat atcattgaaa ccgaaatcat taccgacgca ggaaatatga ctaacgtcag
     2761 aaatagcaat cgccgggtag cccggacggt tttcacggta gcgaccggtc aactcttcgg
     2821 caaagtgcat agcgtcgcaa tgggaaccgc cgttgccgca ggaaagcact ttgccaccgg
     2881 ctttaaagct gtctgctaac aggaccgccg cgcgctgaat ggcgtgaata ttggcgtcat
     2941 cttttaaaaa gttagccagc gtttccgccg cttcgttcag ttcgttacga ataagatcct
     3001 ggtacatgag gatatccttc agcataaatg taatagacaa aatgcagtgt accggatacc
     3061 gccaaaagcg agaagtacgg gcaggtgcta tgaccaggac tttttgacct gaagtgcgga
     3121 taaaaacagc aacaatgtga gctttgttgt aattatattg taaacacatt gctaaatgtt
     3181 tttacatcca ctacaaccat atcatcacaa gtggtcagac ctcctacaag taaggggctt
     3241 ttcgttatga tgattttgag tattctcgct acggttgtcc tgctcggcgc gttgttctat
     3301 caccgcgtga gcttatttat cagcagtctg attttgctcg cctggacagc ctccctcggc
     3361 gttgctggtc tgtggtcggc gtgggtactg gtgcctctgg ccattatcct cgtgccattt
     3421 aactttgcgc ctatgcgtaa gtcgatgatt tccgcgccgg tatttcgcgg tttccgtaag
     3481 gtgatgccgc cgatgtcgcg cactgagaaa gaagcgattg atgcgggcac cacctggtgg
     3541 gagggcgact tgttccaggg caagccggac tggaaaaagc tgcataacta tccgcagccg
     3601 cgcctgaccg ccgaagagca agcgtttctc gacggcccgg tagaagaagc ctgccggatg
     3661 gcgaatgatt tccagatcac ccatgagctg gcggatctgc cgccggagtt gtgggcgtac
     3721 cttaaagagc atcgtttctt cgcgatgatc atcaaaaaag agtacggcgg gctggagttc
     3781 tcggcttatg cccagtctcg cgtgctgcaa aaactctccg gcgtgagcgg gatcctggcg
     3841 attaccgtcg gcgtgccaaa ctcattaggc ccgggcgaac tgttgcaaca ttacggcact
     3901 gacgagcaga aagatcacta tctgccgcgt ctggcgcgtg gtcaggagat cccctgcttt
     3961 gcactgacca gcccggaagc gggttccgat gcgggcgcga ttccggacac cgggattgtc
     4021 tgcatgggcg aatggcaggg ccagcaggtg ctggggatgc gtctgacctg gaacaaacgc
     4081 tacattacgc tggcaccgat tgcgactgtg cttgggctgg cgtttaaact ctccgacccg
     4141 gaaaagctgc tgggtggcgc agaagattta ggtattacct gtgcgctgat cccgaccacc
     4201 acgccgggcg tggaaattgg tcgtcgccac ttcccgctga acgtaccgtt ccagaacgga
     4261 ccgacgcgcg gtaaagatgt cttcgtaccg atcgattaca tcatcggtgg gccgaaaatg
     4321 gccgggcaag gctggcggat gctggtggag tgcctctcgg taggccgcgg catcaccctg
     4381 ccttccaact caaccggcgg cgtgaaatcg gtagcgctgg caacgggcgc gtatgctcac
     4441 attcgccgtc agttcaaaat ctctatcggt aagatggaag ggattgaaga gccgctggcg
     4501 cgtattgccg gtaatgccta cgtgatggat gctgcggcat cgctgattac ctacggcatt
     4561 atgctcggcg aaaaacctgc cgtgctgtcg gctatcgtta agtatcactg tacccaccgc
     4621 gggcagcagt cgattattga tgcgatggat atcactggtg gcaaaggcat tatgctcggg
     4681 cagagcaact tcctggcgcg tgcttaccag ggcgcaccga ttgccatcac cgttgaaggg
     4741 gccaacattc tgacccgcag catgatgatc ttcggacaag gagcgattcg ttgccatccg
     4801 tacgtgctgg aagagatgga agcagcgaag aacaatgacg tcaacgcgtt cgataaactg
     4861 ttgttcaaac atatcggtca cgtcggtagc aacaaagttc gcagcttctg gctgggcctg
     4921 acgcgcggtt taaccagcag cacgccaacc ggcgatgcca ctaaacgcta ctatcagcac
     4981 ctgaaccgcc tgagcgccaa cctcgccctg ctttctgatg tctcgatggc tgtgctgggc
     5041 ggcagcctga aacgtcgtga gcgcatctcg gcacgtctgg gggatatttt aagccagctt
     5101 tacctcgcct ctgccgttct gaagcgttat gacgacgaag gccgtaatga agccgacctg
     5161 ccgctggtgc actggggcgt acaagatgcg ctgtatcagg ctgaacaggc gatggatgat
     5221 ttactgcaaa acttcccgaa ccgcgtggtt gccgggctgc tgaatgtggt gatcttcccg
     5281 accggacgtc attatctggc accttctgac aagctggatc ataaagtggc gaagatttta
     5341 caagtgccga acgccacccg ttcccgcatt ggtcgcggtc agtacctgac gccgagcgag
     5401 cataatccgg ttggcttgct ggaagaggcg ctggtggatg tgattgccgc cgacccaatt
     5461 catcagcgga tctgtaaaga gctgggtaaa aacctgccgt ttacccgtct ggatgaactg
     5521 gcgcacaacg cgctggcgaa ggggctgatt gataaagatg aagccgctat tctggtgaaa
     5581 gctgaagaaa gccgtctgcg cagtattaac gttgatgact ttgatccgga agagctggcg
     5641 acgaagccgg taaagttgcc ggagaaagtg cggaaagttg aagccgcgta attgaataac
     5701 ggagccgaaa ggctccgttt ctttatccgc taattattta aaattaaagc catccggatg
     5761 gttttccagg ctgccggtca acgccgcgaa cagcaccgtt ttaccatcaa tcgaaagcgc
     5821 atcgttcaca ttcagccagg tgagtttctc ttgcgacgtt ttctcatcaa tagtcgagaa
     5881 cagccccgtc atctgattag atttctcgga ccacatcaca gcgatacgtt gcgagccaca
     5941 gtcatgcggt ttgcacgcgc tcatcacctg atacgtctca tctcccaacg ttacggtttg
     6001 tgcgggagta taagtaccgc ctttcatcac ccaggcaggc agcttatgcc cttgtaccat
     6061 ctgattaaat gcagctttgg tggtttcgcc ctttgcaagg ctgctaatgg ttaaatcatc
     6121 ctgcgccatt gcactggtgg cgatgaccag agcggcgact gtcgttattg ccttaaacat
     6181 cattcctccc gagcttatcc tgcccatgtt attaaccttc taaagcctgg cgataacacg
     6241 ataatttgca aatcagatat ttccaatatc agcatttgtt atcaatttat tctgcgctaa
     6301 ggcaactgcc tgataaggtg ttaaagttat aaaacccttc gcataaagga gttgtacacc
     6361 gtgcctggtt tgaagattac gcttttgcag caaccactgg tgtggatgga tggccctgcc
     6421 aacctgcgtc attttgatcg tcaactggaa ggtattaccg ggcgcgatgt gatcgttcta
     6481 ccggagatgt ttaccagcgg ctttgccatg gaagcggcag cttcgtcgct agcacaagat
     6541 gacgtagtga actggatgac agctaaggcg cagcagtgca atgcgctgat tgcaggcagt
     6601 gttgcattac aaacggagtc tggttcggtt aaccgctttt tgctggttga gccgggcggc
     6661 acggtacatt tttatgataa gcgtcatctg ttccgcatgg cagatgagca tctacattat
     6721 aaagcgggca atgcgcgagt gattgtggaa tggcgcggct ggcgtatttt gccgctggtg
     6781 tgctacgact tacgttttcc tgtgtggtcg cgcaatctca acgattatga cctcgccctg
     6841 tacgtcgcca actggcctgc tccgcgctct ctgcactggc aggcattgct gacggcccgc
     6901 gcgattgaga atcaggcgta tgtggcggga tgcaatcgcg tcggcagcga tggcaacggc
     6961 tgccattatc gcggtgacag ccgggtgatt aatccacaag gcgagattat cgctactgcc
     7021 gacgcgcatc aggcaacgcg cattgatgcc gagctgtcga tggcggtgtt aagggaatat
     7081 cgcgataagt ttccggcatg gcgggatgcg gatgagttta ggttgtagtg agtgggggct
     7141 gtatttaggg cggggaattc agaaagggat gtttagtgtg ctgagcgaga gttaaataat
     7201 aaaaaactgg gaagatttaa taatcccccc gcagggcaag agcatgaatt aaatttgtgt
     7261 taaatttttc attttatcag ttcgaaaccc aagctctgag caggatgcaa gcacttcttc
     7321 tatataacat gtgtttaacg
//
LOCUS       DAZGLJ010000219         7339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000219 DAZGLJ010000000
VERSION     DAZGLJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.219"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..362)
                     /locus_tag="ACHK49_004090"
     CDS             complement(<1..362)
                     /locus_tag="ACHK49_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO9264630.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHAT"
     gene            complement(375..2156)
                     /gene="gcl"
                     /locus_tag="ACHK49_004091"
     CDS             complement(375..2156)
                     /gene="gcl"
                     /locus_tag="ACHK49_004091"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO9264631.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(2246..3061)
                     /gene="allR"
                     /locus_tag="ACHK49_004092"
     CDS             complement(2246..3061)
                     /gene="allR"
                     /locus_tag="ACHK49_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO9264632.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(3139..3621)
                     /gene="allA"
                     /locus_tag="ACHK49_004093"
     CDS             complement(3139..3621)
                     /gene="allA"
                     /locus_tag="ACHK49_004093"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO9264633.1"
                     /translation="MKLQVLLLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            3851..4777
                     /gene="allS"
                     /locus_tag="ACHK49_004094"
     CDS             3851..4777
                     /gene="allS"
                     /locus_tag="ACHK49_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO9264634.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            4846..5940
                     /gene="mnmH"
                     /locus_tag="ACHK49_004095"
     CDS             4846..5940
                     /gene="mnmH"
                     /locus_tag="ACHK49_004095"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO9264635.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            6056..6421
                     /gene="ylbG"
                     /locus_tag="ACHK49_004096"
                     /pseudo
     CDS             6056..6421
                     /gene="ylbG"
                     /locus_tag="ACHK49_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(7188..7339)
                     /locus_tag="ACHK49_004097"
                     /pseudo
     CDS             complement(7188..7339)
                     /locus_tag="ACHK49_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1882 a         1911 c         1744 g         1802 t
ORIGIN      
        1 gttgcgtgaa tctgttcact gctgaaacca gccggcgttt taccgaccag acagttaatt
       61 tttttattac ccagcgcacg ggcataacga atcgctgctg ctacgccatc ccgaaactct
      121 tcttcacggc caggaataca ggcaataccg cgctcccccg ccgcccagtc acctgccggt
      181 aaattgtgca gcgtatgttc gagtttatta ctcgccagca cctgttttaa ttcttcaatg
      241 tcgtagtcat aaggaaacat aaattcaacg ccgcgaaaac cacactgcgc agctttctca
      301 aaacgggcga gaaaatcata ttcaccaaat aacatcgata aattagcaga gaaacgtaac
      361 attatttatc tcccttattc atagtgcatg aagcaggttt cagtcggtgc gtccgctgcg
      421 ttatcggcga tatcttcaaa ttccatgacg ttatccagtt cgctgcccat cgaaatattg
      481 gtcacacgct caagaataac ttccacgact accggtaccc gatattgcgc cattaaggct
      541 ttcgcctgtt caaaggctgg cgcaatatct tccggtttga agacccgaat agctttacaa
      601 cctaaacctt ccgctacttt tacgtggtcg acgccgtaac cgttcacttc actggagtta
      661 atattttcga acgcgagctg tacgcagtaa tccatgtcaa aggcgcgttg tgactgacga
      721 atcagcccca gataagcgtt attgaccagt acatggatgt acggaatgtt gaactgtgcg
      781 ccaacggcta attcttcaat caggaactgg aagtcgaagt caccggaaat cgccaccaca
      841 ttccgttctg gatcagcagc acacacgccc agcgccgccg gaatggtcca gcccagcggg
      901 cctgcctgac cacagttgat ccagtgacgg tctttaaaga catgcagcat ttgtgccgca
      961 gcgatttgtg acaggccaat ggtggtgaca taacaaacat cgcgaccaaa ggctttgttc
     1021 atctcttcat acacgcgctg cggtttcacc ggcacgttgt cgaagtgggt tttgcgcagc
     1081 aaagtgcgtt tgcgctgctg gcagtcggcg acccattctt tacgacacgg cagacgaccc
     1141 gctttttgca tctcctgcgc cacttcaacc agcagtgtca gcgccgcttt agcatcagag
     1201 acaatgccga gatccggaca cagcacgcga ccaatttgcg tcggctcaat atcgatatga
     1261 acgattttgc gcccttcggt gtatttctct accgaaccgg tatgacggtt agcaaaacgg
     1321 ttaccgatac caaacaccat gtcggacgcc agcagcgttg cgttaccgta acgatgcgcg
     1381 gtttgcagac ccaccatccc ggccatcagt tcatgatcgt ccgggataca gccccagccc
     1441 atcagcgttg ggatcaccgg aacgctggtc agttcagcaa actgttgtaa cagtgcagct
     1501 gcgtcagcat taattacccc gcccccggca acaatcaccg gacgttcggc ctggattaac
     1561 atttctacag ctttttcgat ctgcatacgg ctggcagcag gtttgtagac cggcagcggt
     1621 tcgtacatat caggatcaaa ctcgatttcc gcaacctgaa cgtcgaacgg taaatccacc
     1681 agtaccggac ccggacgacc agaacgcatc aggtgaaatg cctgttgcaa cacacgaggc
     1741 accagcgccg cttcacgtac tgtaaccgcc attttactta ccggcttggc gatagcttcg
     1801 atatcaacag cctggaaatc ttctttatgc agacgggcgc gcggagcctg gccagtaata
     1861 cacagaatag gaatggaatc agcagaagca gaatagagcg cggtgatcat gtccgtgccc
     1921 gcaggaccgg aagtccccag acatacgccg atattccctg ccgttgcgcg ggtataacct
     1981 tccgccatgt gcgaagcacc ttccacatga cgcgccagaa tgtgacgaat accgccgtgc
     2041 ttacgcatcg ctgagtagaa cggattgatt gcagctcccg gaacgccgaa ggcggtggtg
     2101 ataccttctt tctccagcac atacattgcc gcgtcaacgg ctctcatttt tgccatttta
     2161 ttccaacctc tattaattgg aaaaattttc caactttcaa attttgtcaa aaaaacccgc
     2221 tccgcagagc gggatgcgac agacactatg gatgtgcttt cagtcccaac gccgtgctga
     2281 tatcgcgggc ggtgtctctg accagctcac cctgactgac aaaacgatct tctgtcagtc
     2341 ttgatgaagg cccggagatg gagatagcgg caacaacgct accgacatca tcgtaaattg
     2401 ctgaagctat gcaattcaga cctacaacat gctcttcttt atctacggta tagcccagtt
     2461 cacgcgcttg ttccaggtcc ttcagcaagg tgggcatatc cacaagcgta gttggcgtaa
     2521 actgctgcaa accggtttgc agaatgatgc tcatcaactc ctcttccgcc agcggataaa
     2581 gcaacgcctt gcccgcaccg gaagcatgca gcggcagacg actgcccagt ggcgcacaca
     2641 tcctgaccat cgatttacac tctaactgac caattaatac cgcttcattg ccgttacgga
     2701 tcgcgacatt gaccgtttcg ccggaaagta acatcaggcg gcgcataaac ggcccggcga
     2761 cggagaggac atcgcggtta tggatgtacg ccgcaccgac gttaaagaca cctaatccta
     2821 tatgccacca gcctaactga ctgtcctgat agacaaaatc cgctgcctgt aaaaccttca
     2881 gcaagcgaaa ggtcgtagag agcggcaaat ccagattgag agaaatatcg ctaaccgacg
     2941 aacttccccc acttttttcc agatattgca gaatcgcaat tccccgctct aacgcctgtg
     3001 cgcccttctg tgccacaggc tccgcctgtc ctggcctgcc gcgccgtcta acttccgtca
     3061 tacctacgtt aactccgacc tacaagtgag cataaacaat gaacaattag tttatcttat
     3121 gcaagtcggt tgcaggcgtc acgcaaaaca gagttcctgt tcaggaatac tttcaacatc
     3181 acagttgtca ctgccgccgc gatcgatggt cagaaaatcg gtgacgcgct gccaggcgaa
     3241 aagtgggtga tgccagacgt tacggtggta attcactccc tgttcgccgt tggtaataaa
     3301 cgcccgcagg gttgacaggt ctggtttgtc gtcacctaac gccacgacca ccacaaacac
     3361 ctcacctttc atcgggataa aggcctgagt acccagcgga tgacgttcga gttcgtgaat
     3421 ggtcagcggc agattcgccg gttgcgcgcg gttaatgctg ataagcgtac ggtcttgctc
     3481 aagaatctca accagcgcca aatcgtggta acgctccacc aggccattgt taatatggaa
     3541 aaaatcccgt tgctgcgttt cgattacgtc gccataagca ctaaaggctt cctgacttaa
     3601 cagtaatacc tgaagtttca tctttttgtc cttgtaacat aattccgttc aaccaccgcc
     3661 gaccggtcag cttggtttca ataacaggca atctaaaacc ttaagagaac aacatcaata
     3721 caaaagttgg aatatttttc caaaactaga tttagtccac aaattgaaca gggttatcat
     3781 gtaaaaatac tgataaatca gaggataaga aaatcacgca acttgcggat ttaaaagggg
     3841 atacctgaag atgttcgatc cagaaacctt gcggactttc attgcggttg ctgaaacagg
     3901 aagtttttca aaagcggcag aacgattatg taaaaccacg gcgacgatca gttatcgcat
     3961 taaacttctg gaagagaata ccggagtagc gctgtttttc cgcacgactc gcagcgtgac
     4021 gttgacagcg gctggcgagc atctactttc ccaggccaga gactggctga gctggctgga
     4081 aagtatgcca agcgagctgc aacaggtgaa tgatggcgtg gaacgccagg tgaatattgt
     4141 catcaacaac ctgctctaca acccccaggc cgtcgcccag ttgctggcgt ggctgaatga
     4201 acgttacccc tttacccagt ttcacatctc ccgacaaatc tatatgggcg tctgggattc
     4261 gctattatac gaaggttttt cactggctat cggcgtcacg ggaaccgagg cgctggcaaa
     4321 tacctttagt cttgatccct taggatcggt gcaatggcgc tttgtcatgg cggcggatca
     4381 tccgctggcg aacgttgaag agccgctaac agaagcgcag ttgcggcgct ttccggcggt
     4441 caatattgaa gacagcgccc gcaccttaac caaacgcgtc gcctggcgat tgcctgggca
     4501 aaaagagatt attgttcctg atatggaaac gaaaatcgcc gcccatcttg cgggcgttgg
     4561 cattggtttt ttgccaaaat cgctttgcca gtcaatgatc gataatcaac aactagttag
     4621 ccgggtaatc ccaacgatgc gccctccttc gccattgagt ctggcatggc gcaaatttgg
     4681 cagcggcaaa gcggtagaag atattgtgac cttgtttacc cagcgcaggc cggaaatcag
     4741 cggattttta gaaattttcg gcaacccacg cagttaaaaa tcaagtttcc ttgcgcgttg
     4801 gcgcgtaaaa tagcgcgcaa gctgatttga ggttcacttt taactatgca agagagacac
     4861 acggaacagg actatcgtgc cctgctgatt gctgatacgc ccattattga tgttcgcgcc
     4921 cctatcgagt ttgagcaagg cgcaatgccc gccgctatca atctgccgtt aatgaataac
     4981 gatgaacgcg ccgccgttgg cacctgctat aaacagcaag gctcagacgc agcgctggcg
     5041 ctggggcata aactggtggc gggtgaaatt cgtcagcagc gcatggacgc ctggcgggca
     5101 gcgtgcctgc aaaatccgca aggtattctc tgctgcgccc gtggcggtca gcgctcacat
     5161 attgtgcaaa gctggttgta tgcagcgggg attgattatc cgctggtgga aggcggttat
     5221 aaggcactgc gccagaccgc gattcaggcg actattgaac tggcacaaaa accgatagtg
     5281 ctgattggcg gttgtactgg cagcggtaaa acgctgttag tgcagcaaca gccgaacggt
     5341 gttgatctgg aagggctggc gcgccatcgc ggttcggcgt ttggtcgcac gttacaacca
     5401 caacttagcc aggcgagttt tgaaaacctg ctggctgccg aaatgctgaa aaccgacgcc
     5461 cgtcaggatt tgcgcctgtg ggtgctggaa gacgaaagcc ggatgatcgg ttcgaatcac
     5521 ctgccggaat gcctgcgcga gcgaatgact caggcggcga ttgcggtggt agaagatccg
     5581 tttgagatcc gtcttgagcg cctgaacgaa gagtatttct tgcgtatgca tcatgatttt
     5641 acccacgctt atggcgacga acagggctgg caggaatatt gcgaatacct gcatcacgga
     5701 ctttcggcga ttaagcgtcg gctggggtta cagcgctata acgaactcgc tgcccagctg
     5761 gatacggcgc tgacaacgca actcaccacc ggcagcaccg acggtcatct ggcctggctg
     5821 gtgccgttac ttaaagaata ttacgaccca atgtatcgct atcagcttga gaaaaaagcg
     5881 gaaaaagttg tctttcgcgg tgagtgggcg gaagtggcgg aatgggttaa ggcgcggtaa
     5941 tgagggacgt atttgtatta gtcgcgactt gatctgacac atggccttga aaggttgaga
     6001 gttaccggtt ttgatatgag tgtcgaatcc ttatccaaaa caagaggtaa ctctcatgct
     6061 tcatactgcc aatcccgtca tcaaacacaa agccggtttg ctcaatctgg ctgaagaact
     6121 cagcaacgta tcaaaagcct gtaaaatcat gggcgtttcg cgtgatacgt tttatcgtta
     6181 ccgcgaactg gtcgctgaag gcggtgtgga tgcgcagatt aatcgtagtc gccgcgctcc
     6241 taaccttaag aaccgtaccg atgaggcaac tgaacaggct gttgttgatt acgccgtcgc
     6301 tttcccgaca catggtcagc accgggccag caacgagctg cgtaaacagg gggtttttat
     6361 ctccgacagt ggtgtccgtt ctgtctggtt actccataac cttgagaatc tcaaaagacg
     6421 atactgaaca aattttatca ggtgacgttc cgtaaaaagt tgtatggagt tctcgatgca
     6481 gtcggcgcga ctatcagtac ccccgctgaa ccctggacta tcgggccgcc gtactggggc
     6541 atgtcaccct ggcgtgccgc tggttttccg ctaatggttt atttcctctt tctttcccgg
     6601 caagaattta ttttaatccg gaaggattat ttattttcag atagggactc acattaaaca
     6661 cgtaacattt taattaatgc atggctgaat atgtattaca caatgtcaga tatcgtaaaa
     6721 gaccagtgca attctatgta aactctctga ctgttcattt tatttgttgt ttcagggtcg
     6781 gaactcaccc tcaataaaca actgaatacg agtagatatt catcgtctga gctatatggc
     6841 tttacacaat agccgacatg tcttttctca gtcaggaatt ttacttttcc tcactacgaa
     6901 gttgacgact tcgatatggg atagactctt aattcaagca attactctgc acaagcatca
     6961 ggcaatccag ctccaatatt tgttataaaa taatgcgtaa cattaatttc accaacaaaa
     7021 aaaaggttta caccatcccc ttgccctggt atcttaccat atttacacca cacttttttt
     7081 gtatctggat cattctttga tacgattttc gccacttgtt ccctggccct cactgtcaat
     7141 gttttcttaa acctaccccc atcatcattt gttagggaaa aataagaaaa aaaataatca
     7201 tcttctacat ttgtgtatat tgatatatcc accgctacgt gcggaaaaaa aatctgggag
     7261 ccatgtaaaa actccatcat tttctggtat tgccatatag tttatgtaat gtttacttac
     7321 aagagattca tctttgtat
//
LOCUS       DAZGLJ010000220         7330 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000220 DAZGLJ010000000
VERSION     DAZGLJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.220"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            85..1758
                     /locus_tag="ACHK49_004098"
     CDS             85..1758
                     /locus_tag="ACHK49_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO9264636.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            1783..3222
                     /locus_tag="ACHK49_004099"
     CDS             1783..3222
                     /locus_tag="ACHK49_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO9264637.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(3279..3497)
                     /gene="marB"
                     /locus_tag="ACHK49_004100"
     CDS             complement(3279..3497)
                     /gene="marB"
                     /locus_tag="ACHK49_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO9264638.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(3529..3912)
                     /gene="marA"
                     /locus_tag="ACHK49_004101"
     CDS             complement(3529..3912)
                     /gene="marA"
                     /locus_tag="ACHK49_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO9264639.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(3932..4366)
                     /gene="marR"
                     /locus_tag="ACHK49_004102"
     CDS             complement(3932..4366)
                     /gene="marR"
                     /locus_tag="ACHK49_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO9264640.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            4578..5243
                     /gene="marC"
                     /locus_tag="ACHK49_004103"
     CDS             4578..5243
                     /gene="marC"
                     /locus_tag="ACHK49_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO9264641.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(5268..6458)
                     /gene="ydeA"
                     /locus_tag="ACHK49_004104"
     CDS             complement(5268..6458)
                     /gene="ydeA"
                     /locus_tag="ACHK49_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO9264642.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(6608..>7330)
                     /gene="yneK"
                     /locus_tag="ACHK49_004105"
     CDS             complement(6608..>7330)
                     /gene="yneK"
                     /locus_tag="ACHK49_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO9264643.1"
                     /translation="PKPVTMGRVFQTGVERVLFLFLNDFIEQFPMINLGVPIKRAHTP
                     HIEPLPPDRHTAADYLRQFDLLVLNFISRGNFVILPRLWSNSEVHRWFVNKDPNLITA
                     ILDITDGELKEDLLQSLMDSLGSNKHVLPEVCICFLSLLAEQESPHFQDFFLFFANML
                     LHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKVTHEDLVKNR
                     PRSPVRPPIPATAKTPDLPERH"
BASE COUNT         2038 a         1582 c         1778 g         1932 t
ORIGIN      
        1 tttcattaag aaaagaactc catgcctgat tttttcctcc agtgcgatag ctggaggaaa
       61 ttattatact cttaaggata agtcatgaat ataaagacgc ttaacgtcag ccttttgtct
      121 ttttctatta ttacagcatt gtttccattg aacgcgatgg caacaaaatt aaccatagag
      181 cagcgccttg aattgcttga aaatgaattg tcgcaaaata aacaagagct gaaagcaaca
      241 cagaatgaac tgggagtata taaatcccga ctttcgacct tacaaaaaag catcacggaa
      301 aacaaatata aatcagcctc gcttgctgaa atatcagcga catctcccgt cgctgataac
      361 atcaaaaatg aaaacggtga acagaactcg tttaccgcag cgcatactat aaatggatcg
      421 cagcaagttg ccgttattga aagtaaaggc gacaaaacca ctatcgaaag cgtgaccctg
      481 aaagatatca gtaaatatat aaaggatgat attgggttca gctatcaggg gtacttccgc
      541 tcaggctggg gtactggtaa tcacggctca ccacaaactt atgcagcggg ttctctggga
      601 cgttttggta atgagatgag cggttggttt gacctgacct taaatcagcg tgtttataat
      661 caggacggta aaacggcaaa tgcggtcgtt acctatgatg gcaacgtggg tgagcagtat
      721 aacgatgcct ggtttggtga cagtgccaat gaaaatatca tgcagttcag cgatatttac
      781 ctgacaacgc gaggtttttt acccttcgca ccagaggcag acttctgggt cggcaaacat
      841 aaactcccgc aatatgagat ccagatgctg gactggaaaa ccttaaccac ggatgtcgcc
      901 gcgggtgtgg ggattgaaaa ctgggcactt ggtgtagggc tgtttgatat gtccttaagc
      961 cgtgatgatg tcgatgttta ctctcgtgat ttttcgcgta ccagccagat gaataccaat
     1021 tctgtggatg ttcgttatcg caatatcccg ttatgggatg atgcgacatt atcattaatg
     1081 gctaaatatt ccgcacctaa taaaacggat caacaacaag ataacgaaaa tgacaacagt
     1141 tattttgaaa tgaaagatag ctggatgctg acttctgttt tacggcaaaa cctgcaacgc
     1201 gatacgttta atgaatttac gttacaggtt gccaataatt cctatgccag cagttttgcc
     1261 agtttctcag atgccagtaa tacgatggcg catggtcgct attactatgg tgatcatacc
     1321 aatgggatcg cctggcgttt aatctctcag ggcgagatgt atcttactga caatattatt
     1381 atggctaacg cgcttgtcta ttctcacggc gaagatgttt atagttatga aagtggtgct
     1441 catagtgatt ttgacagtat tcgcactgta ataagaccgg cctggatctg gaatacatgg
     1501 aatcagacgg ggcttgaatt aggctggttt aagcaacaga acaaaactca gcagggtgta
     1561 acactaaatg aatcggctta taaaacgaca ctctggcatg cattgaaagt gggtgaaagc
     1621 attttaggtt cacgaccaga aattcgcttc tatggaacgt atatcaatat tctggataac
     1681 gaattatcga attttaagtt taatgagaac agcaaagacg agtttatggc cggcatccag
     1741 gcggaagtct ggtggtaatc tattttagtt aaggagttgt gtatgtcagg atttaaaaaa
     1801 ggttttttat ggggcggtgc ggtagccgcg catcagttgg aaggtggctg gaatgaagga
     1861 ggaaaaggca tcagcatcgc tgatgtgatg actgctggcg ctcacggggt gccgcgtgaa
     1921 gtgacagaag gcgttatcga cgggcttaat tatcccaatc atgaagcaat tgatttttat
     1981 catcgctata aaacagatat tcagttattt gccgagatgg gattcaaatg ctttcgaact
     2041 tccattgcct ggacacgaat ctttccgcaa ggtgacgaac aggagccgaa tgaagagggt
     2101 ttacaatttt atgatgatct gttcgatgaa tgccttaagc agggaatgga acctgtggtg
     2161 acgctttcac attttgagat gccttatcat ctggtgacaa aatatggtgg ctggcgaaac
     2221 cgtaaactga tcgacttttt tatccgcttc gcatcaacgg tcttcacgcg ctataaagaa
     2281 aaagtaaagt actggatgac gtttaacgaa atcaataatc aggtgaattt cagcgaaagc
     2341 ctgtgtccat ttactaattc cggtatcttg tattcgccag aggaagatat caatgagcgc
     2401 gaacaaataa tgtaccaggc ggtacattac gagttagttg ccagtgccct ggcggtacag
     2461 actggaaaat cgatcaatcc tgaatttaac attggctgta tgatcgccat gtgtcccatc
     2521 tatcctctga cgtgtgcacc caacgatatg atgatggcca cgaaagcaat gcatcgtcgt
     2581 tactggttta ctgatgttca tgctcgtgga tattatccgc aacatatgct gaattacttt
     2641 gccaggaaag gattcaacct cgatatcaca ccagaagata acgcgattct tgccagtggt
     2701 tgtgtcgact ttatcggctt tagctactac atgtctttta cgacacaatt ttcgcccgat
     2761 aacccgcaac tggattatgt tgaaccacga gatttggtca gcaaccctta tatcgataca
     2821 tccgaatggg gatggcaaat tgatccggca gggctacgtt attcactcaa ctggttctgg
     2881 gatcatttcc agttgccgct gtttattgtc gaaaatggat ttggtgcggt tgaccagaga
     2941 caagctgacg gcacggtgaa cgatcactat cgcattgatt actttgcttc ccatattcgg
     3001 gaaatgaaaa aagccgtagt tgaagatggt gttgacttaa ttggctacac cccgtggggc
     3061 tgcattgacc tggtttctgc cggaacaggg gaaatgaaaa agcgctacgg aatgatttat
     3121 gtcgataaag acaacgaagg gaagggaacg ctggaacgga tacgtaaagc gtcgttttac
     3181 tggtatcggg atctcatcgc caacaatggc gaaaatattt gagacttgag gaagatatta
     3241 cggagatgtc aggctctgtg atcagagcct gacaataact acatagcgtg ttgattataa
     3301 tagggcacgc cgagcgcatc cgacttatca ctgccagtac ccatgtgatt taaatcaaac
     3361 ggtggttgtt cctgcgatgg gggaacaacc acgacattgg cacaagaagt aacaactggc
     3421 tgcgtggttt gttccgcaac gccctgcgcg gaaaagagaa taagcgcagc tgctattgcg
     3481 gatgaaagtg gtttcatgat tgcctcagtg acgttgtcac gttatcaact agctgttgta
     3541 atgatttaat ggatgtaaaa agcgcgattc gccctgcata ttggtcatcc ggtatttatg
     3601 cggcggaaca tcaaagtaat ttttgaaggt tcgggtcaga gtttgttgcg actcgaagcc
     3661 atatcgttct gccagataga gtatcggctc gttactttcc ttcagctttt gcgcgatttc
     3721 cgtcatctta cggctgcgga tgtattggcc taatgaatga ccggtttctt ttttaaacat
     3781 ccgttgcagg tgccatttag agtaacctga acgctctgac actttctcca gtgacagtgg
     3841 cgattccagg ttgtcctcga tccagtccaa aatgctatga atggtaatag cgtcagtatt
     3901 gcgtctggac atcgtcatac ctcttttttg tttacggcag gactttctta agcaaatgct
     3961 caagtgttgc cacttcgtcc gccgtcaggt tttttgttaa ttcttgatgc aggtcctggc
     4021 caactaattg atggcattgt tcgcatattg ccgcgccgct ggtggtaagt tttaccagta
     4081 cgccgcgctt atcattcggg ttcggcaacc tttcaaccca gcctttacag accaggcgat
     4141 ccagcatacg ggtcagtgct ccaaggtcga cagacaacac ttttttcagt tcaaccggag
     4201 taatacacgc cgcgcagcgg atagagcaga gcaccttaaa ctgtgccgcg gtaatatcca
     4261 gcggagacag atactcgtta agcagacgat ctttcttctg attaaccata tggattaagc
     4321 gacccaatgg aataatttca ttgaacagat cgctggtact tttcacatta gttgccctgg
     4381 caagtaatta gttgcagggg ataatattgc ccaggcaagt ataagtcaaa taaatgaatt
     4441 gaccgatgcc acgttttgct aaatcggttc aagttttggt caatgcgatg caaggttagc
     4501 taaagagtga cactttatcc ttgtttaccc cttatacttt tcgctgataa cccagataca
     4561 caggataaca accaccaatg ttagatttgt ttaaagcaat tggcctgggg ctggtggtgt
     4621 tactgccgtt agctaaccca ttaacaactg tcgcgttatt tcttggcctg gcaggcaaca
     4681 tgaacagtgc cgaacgtaat cgtcagtcgt tgatggcctc ggtgtacgta tttgccatca
     4741 tgatggtggc gtattacgcc gggcaactgg tgatggatac atttggcatt tcgattcccg
     4801 gtctgcgaat tgcaggcggc ttaatcgtcg cgtttatcgg ttttcggatg ctctttccgc
     4861 aacagaaagc gattgattca ccggaggcga aaagcaagtc ggaagagctg gaagatgaac
     4921 ccagtgccaa tattgccttt gtgccgctag caatgccaag taccgctggt ccgggaacca
     4981 tagcgatgat tattagctcg gcgtccacag tgcgtcagag ttcaaccttc gctgattggg
     5041 tactgatggt tgcgccgccg ctgatctttt tcctggtggc ggttattttg tggggaagcc
     5101 tacgcagctc cggcgcaatt atgcggctgg tgggcaaggg tggcattgaa gcgatctccc
     5161 gtctgatggg attcctgctg gtatgtatgg gtgtgcagtt tattattaac ggcatcctgg
     5221 aaatcattaa aacgtaccat taaaaaaggc ccgaatgggc ctttcaacta ttgcgtctgt
     5281 tcttcgagtg tcactggcca gcggcgaaat ataatgattg accaaattaa cgcggcaaaa
     5341 gcaggcaccg cgcccacata accaatcatc gacattgacc agtgcaaact cacctgatta
     5401 cctaccaacg cacccgcccc gattccaata ttaaatatcc cggagaatag cgccatcgcg
     5461 acgtcggtag catctggtgc cagcgccagc actttaacct gcataccaag cccgatgatc
     5521 atcatcgcga tcccccagaa aatactcagc accccgaggt gtatttcact gttcgccgca
     5581 ggtaacagca atgccaggca caccagcaac agcgcaatcg ccgtactcac caacgcagac
     5641 gcatactgat tacccagttt accgaaaatc acgctgccaa taatgcccgc accaccgagt
     5701 aataacagta atgccgtggc aaagttggcg ctgaatcccg caatgttttg tacaaaaggc
     5761 tcgatatagc tgtatgccgt gtaatgggcg gtgacaacca ccacagttaa caaataaatg
     5821 ctcatcaatg ccgggcggcg gaacaatagc gggaggcttt tcagtgaacc ggaatgctca
     5881 ctgggcagta agggaagtaa cttaatcagg cacaaaaggg tgataagcgc cccaatacca
     5941 atcgcgaaga aggtcattcg ccaaccgaaa tactggccca caatgcgccc gagaggtaaa
     6001 cctaagacca tcgccagtgc tgtaccggtg gcaattaaac tcaatgcctg tgctcgcttc
     6061 ccggccggag ccatacggat cgccagagac gccgtaatcg accagaaaat cgcatgtgca
     6121 aaagccacac caatgcgact gatcaccaga acggtaaagc tccacgataa aaacgacagt
     6181 acgtggctgg caataaacac cacaaacagg cagatcagta atttgcgccg ttcaacctgg
     6241 ctggtcatta acataaaagg caatgacatt agcgccacta cccatgcata aatggtcaac
     6301 ataatgccga cctgagcggt ttgcatgtga aaactttgcg caatgtcaga gagcaggcca
     6361 acagggacaa attcggtggt gttgaagatg aaggcggcga ctgccagcgt aacgacccgt
     6421 agccacgcca ctttgcggga aacagtgttt gttgtcataa cagattatcg ggtgagttgc
     6481 gaaaaagtga tgaaatgatc ttaaaacggt taatgatgtg aatacaatgc atttgtgatc
     6541 tggatcgcgt tttccgtttc tctgacgctg attctgttcc gcgtcagaga gtcggtaaca
     6601 tcacggttta atgacgttca gggaggtctg gcgttttggc ggttgcgggt ataggtggtc
     6661 tgacagggga gcgaggcctg tttttcacca ggtcttcgtg agtaactttt ggcggcgttt
     6721 cattttttac aaacagtttg agggtcctgc gcgccatatg tttgataatt ttatcatcac
     6781 ttaatgatgc tggcatcaac acgtcattca gatcactttc attgggattc ataaattggt
     6841 gatagtgcaa taacatattg gcgaaaaata aaaaaaagtc ctgaaaatga ggagattctt
     6901 gttctgctaa aagggataaa aagcagatgc agacttcggg tagtacatgt ttgttagaac
     6961 ctaatgaatc catcaggctt tgcaataaat cctcttttaa ttcgccgtct gttatgtcga
     7021 gaatggcggt gatcaagtta ggatctttat tgacgaacca tctgtgaacc tcagagttac
     7081 tccataatcg ggggagtatg acaaaattac cgcgagagat aaaattcagg acaagcaaat
     7141 caaactggcg taagtaatct gcagcggtat ggcgatccgg aggcaggggt tcaatatgtg
     7201 gtgtatgcgc tcttttgatg ggaacgccaa ggttgatcat tggaaattgt tcaataaaat
     7261 cattcagaaa cagaaatagc acacgctcga cgccggtctg aaatacgcgc cccatagtga
     7321 caggctttgg
//
LOCUS       DAZGLJ010000221         7305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000221 DAZGLJ010000000
VERSION     DAZGLJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.221"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(289..1482)
                     /gene="tyrB"
                     /locus_tag="ACHK49_004106"
     CDS             complement(289..1482)
                     /gene="tyrB"
                     /locus_tag="ACHK49_004106"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO9264644.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKSRELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1717..2814)
                     /gene="alr"
                     /locus_tag="ACHK49_004107"
     CDS             complement(1717..2814)
                     /gene="alr"
                     /locus_tag="ACHK49_004107"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO9264645.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVDLSFCNI"
     gene            complement(2867..4282)
                     /gene="dnaB"
                     /locus_tag="ACHK49_004108"
     CDS             complement(2867..4282)
                     /gene="dnaB"
                     /locus_tag="ACHK49_004108"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO9264646.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4365..5348
                     /locus_tag="ACHK49_004109"
     CDS             4365..5348
                     /locus_tag="ACHK49_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO9264647.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5514..5756)
                     /gene="pspG"
                     /locus_tag="ACHK49_004110"
     CDS             complement(5514..5756)
                     /gene="pspG"
                     /locus_tag="ACHK49_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO9264648.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5890..6927)
                     /gene="dusA"
                     /locus_tag="ACHK49_004111"
     CDS             complement(5890..6927)
                     /gene="dusA"
                     /locus_tag="ACHK49_004111"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO9264649.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNTQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
BASE COUNT         1868 a         1920 c         1786 g         1731 t
ORIGIN      
        1 taaggcaaag catattcttg aggatataga aatattctat cgtaacaaac ccgatactta
       61 ttattaaatt taataatggt tagatgtgca gaaattatta ctccacccaa aaaaacagga
      121 taaggaattc cagataattg ggataagctt aacaataaaa gaactaacaa cgtttattat
      181 ctctcacgta gaacgatggc atcaaaaaaa tcatctataa agaaagagtg aaagcgacga
      241 cagtttaatt tcactgcagg ctgggtagct ccagcctgct ttcctgcatt acatcaccgc
      301 agcaaacgcc tttgccacac gttgtacatt tgccgtattt aacccggcga cacacatgcg
      361 accgctggcg atgagataga caccaaattc ttcacgtagt cggtcaacct gagcggcact
      421 taaaccggta taactgaaca tgccgcgctg attaagcaga taatcgaaat tgcgttctgg
      481 catctctgtg cttaatacct tcaccagttc ctgacgcatt gccagaatgc gagtacgcat
      541 ctcttctact tccgccagcc agctggcttt caatgcctcg tcattcagca ctgcagccac
      601 cacctgcgca ccaaaattcg gcgggctgga gtagttgcgg cgaactgttg ctttcaattg
      661 ccccagtacg cggccagcgg cttcggcatc ttcacacaga acagaaagtc cgccgacgcg
      721 ctcgccgtaa agggagaaaa ttttcgagaa cgaattgctc accagagcgg gtaatccagc
      781 gctggcaatg gcgcgaatag cgtaggcatc ctcttccata ccggcaccaa atccttgata
      841 ggcaatatcg aggaatggaa taagctcgcg ggatttgaga atttcaatca ccgcatccca
      901 ctgatcatta gtgagatcgg cacccgttgg gttgtggcaa catggatgca gcaacacaat
      961 actgcgggca ggtaatgttt tcagcgtcgc caacaggtca ttaaagcgca cgccgttagt
     1021 cgcttcgtca taccaggggt aagtactcac ttcgaatcca gccccggcga atattgctac
     1081 gtggttttcc caggtaggat cgctgaccca gacgcctgat tccgggaagt agcgtttcag
     1141 gaaatccgcg cccactttca atgcccctga gccgccaagg gtttgaatgg ttgctacgcg
     1201 ctgttgttgc agtactggat ggtccgcacc aaacagcagc ggcgcaatgg catggcgata
     1261 gctgttaagc ccttccatcg gtaaataaag cgaagcgcca tgaggctgcg cattcaggcg
     1321 cgcttccgcc tccgccacgg cttgcagttg tggaataatt ccgtcttcgt tgtagtacag
     1381 accgatactt aaattcactt tgtcgctgcg agggtcttct ttaaaacgct ccataagcgt
     1441 aagaatcggg tcgccagcgt aggcgtcaac tttttgaaac acgcgatggt tctccaggtt
     1501 tacgggcagg tggttaaaac acaataaacc ggaagaaggc gaagatcgag tggatgttca
     1561 ggagcgaacg gcaattagca acagagtgag actcatgaca aacgtacatc cgccagaggc
     1621 acgaccttta taagagcatg aaagaacatc aacttattga atttttagga ttttattggc
     1681 cggataaggc attcacgccg catccggcac agacaatcaa atattacaga acgataaatc
     1741 cacgtatttc atcgcgaccc ttgaagtcag gcgcgtaata agttcgtaag cgcttacttt
     1801 cgtcatttca gcgatacgtt ctacgggcaa accttcgccc cataaaatga ccggatcccc
     1861 ggctttgtcc tgcgcttgtg gacctaagtc tacgcagatc atatccatcg ccacgcgccc
     1921 gacaatcggt acttcgcgac cgttcaccag cactggcgta ccggacggcg cggcgcgcgg
     1981 ataaccatcg ccatagccca tcgcgactac gccaagacgg gtatcacgtt cgcttaccca
     2041 ggttccacca taaccaacag gttctccggc tttatgctca cgcacggcaa tcaggctgga
     2101 ggttagtgac atcactggct gacagccaaa atcggcaccg gtggagcgat cttccagcgg
     2161 cgagacgcca taaagaatga tgcccgggcg cacccagtca aaatgcgact gtggccacag
     2221 cagaatgcca cccgatgcgg caatggaacg ttgaccaggt ttgccttcgc aaaaggtatt
     2281 aaagatagcg agttgtttct cggttgcgcc acattttggt tcatccgcgc gcgcaaaatg
     2341 gctgacgata ttcaccggct gacgaacgtt tttgcactgg gtcaggcgat gataaaacgc
     2401 ctcagcctgt tccggcctta cgcccagacg gtgcataccg gtatcgagtt tcatccagac
     2461 ggtaaccggc tcgtccaggc tagcctcttc cagcgcagcc agctgttctt cgttatgcac
     2521 ggcggtatga aaatgttgcg cagaaatcgt cggcagatct ctggcatcaa aaaagccttc
     2581 gagtaacagt acaggtttgg tgattccccc cgcacgcagt cgcagagctt cttcgagacg
     2641 ggctacgcca aaggcgtcag catcggggag cgttcgcgcg gtctcaagaa gaccgtgacc
     2701 ataagcgttc gctttcacca ccgcaaccat tttactggca ggggccagtt cacgaagacg
     2761 ttgcaggttg tgtcgcagag cgcggcggtt aatcacaaca gttgccgctt gcatttgtgt
     2821 tccttgataa gtgtttgctt taattaccta attcataaaa taattattat tcgtcgtcgt
     2881 actgcggccc cgcatagttg tcgaagcgcg accattgacc gttaaaggtc aggcgtaccg
     2941 tcccgattgg gccgttacgt tgtttaccga taataatttc cgcgatgcct tttaaatcac
     3001 tgttttcgtg atacacctca tcacgataga taaacatgat caagtccgca tcctgctcga
     3061 tagagccaga ttcacgcagg tcggagttga ccgggcgttt gtcggcacgt tgttccagag
     3121 aacggttcaa ctgggacagc gccaccaccg gcacgttcag ttctttcgcc agtgctttca
     3181 gcgagcggga gatttctgca atttccagcg tacggttatc ggaaagcgcc ggtacgcgca
     3241 tcagttgcag gtagtcgatc atgataagcc cgatgccgcc gtgttcacgg gcaatacggc
     3301 gtgcgcggga acgcacttcc gttggcgtca ggccggagga gtcatcgata tagatattgc
     3361 gtttttcgag caaaataccc atggtgccgg aaatgcgcgc ccagtcttca tcatcgagct
     3421 gcccggtacg gattttagtc tggtcaacgc gcgacagcga cgccagagaa cgcatcataa
     3481 tctgttctga aggcatctcc agcgagaaga taagtactgg tttatcctgc aacatcgccg
     3541 cgttttcgac gaggttcatc gcaaatgttg ttttacccat cgacggacgc gcggcgacga
     3601 tgatcaaatc cgacggctgc aagccagcgg tttttttgtt gagatcgtca taaccggtgt
     3661 ttactccggt aacgccatcg tgtggctgct gaaacaactg ctcaatacgc gccacggttg
     3721 cgtcgagcac atcggcgatg ttcttcggcc cttcgtcttt gtttgcacga ctttcggcaa
     3781 ttttaaagac gcgggattca gcaaggtcca gcagatcttc gctggtacgc ccctgcggat
     3841 caaaaccggc ttcagcaatc tcattcgcaa ccgagatcat ctcacggaca acggcacgtt
     3901 cacgcacgat gtcagcataa gcactgatgt tcgccgcact tggcgtattt tttgacagct
     3961 ctgccagata agcaaaacca ccgacgctat cgagttgccc ctggcgttcc agcgattccg
     4021 caagggtaat cagatcgata ggactaccgc tttcctgcaa acgcgccatt tcagtaaaga
     4081 tatggcggtg tgggcgggtg taaaaatcgt ctgccaccac acgctcggct acatcgtccc
     4141 agcgttcgtt atccagcatt aaaccgccca acaccgactg ctccgcttcg atcgagtgcg
     4201 gaggcacttt cagcccggca acttgtggat cgcgttcgcg gggttcagcc tgctgtttgt
     4261 tgaagggttt atttcctgcc atagtgaatg gagttaccga gataaagaat gggtcgaaac
     4321 tttaccatat gaagcagccc ctgacgatac gttctggagg acacatggca acacgaattg
     4381 aatttcacaa gcacggtggc ccggaagtac ttcaagccgt agagttcact cctgccgatc
     4441 cggcagagaa tgaaatccag gtcgaaaata aagccatcgg catcaatttt atcgacacgt
     4501 atatccgcag cggcctttac ccgccgccat cactacccag cggattaggt accgaagcgg
     4561 caggcatcgt gagtaaagtc ggcagtggtg taaagcatat taaggcaggc gatcgtgttg
     4621 tctatgcgca gtcggcgtta ggcgcttaca gctctgtgca taacattaat gcggataaag
     4681 cggcgattct gcctgcggca atttcttttg agcaagctgc ggcatccttc ctgaaaggct
     4741 taacggttta ttatctgctg cgtaaaacct atgaaattaa acccgatgaa cagttcctgt
     4801 tccacgcagc ggctggcggc gttggcttga ttgcctgcca gtgggcaaaa gccctgggcg
     4861 cgaaacttat cggcaccgta ggaaccgcgc aaaaagcgca gagcgcgcta aaagcgggcg
     4921 cgtggcaggt aattaactat cgtgaagaga atctggtcga gcggttaaaa gagatcaccg
     4981 gcggtaagaa agtgcgcgtg gtgtacgatt ccgtgggcag agacacctgg gaacggtcgc
     5041 tggattgcct gcaacgccgc ggcttaatgg tcagttttgg caactcatca ggtgcggtta
     5101 ccggtgtgaa cttaggcatt ctcaatcaaa aaggctcgtt gtatgtgaca cgcccttccc
     5161 tgcaaggcta tatcaccacg cgggaggaat taaccgaggc cagtaatgaa ctgttctctt
     5221 tgattgccag cggtgtgatt aaggtcgatg tcgccgagca gcagaaatat ccgctgaagg
     5281 atgcgcagcg tgcgcatgag attctggaaa gccgggcgac gcaaggttcc agcctgctga
     5341 ttccataaaa gaaatagggc ttccacctgg gaagcccttt ctttttatag ttcggctgta
     5401 tgtagggtac agcacgatga atctgttaga ggcgcaatag tgacagattt gattatcaat
     5461 tcctattttg ttctaaggat aaaaccttag gttgtgatca tccgcacaat cccttagtaa
     5521 cgccagcggt cataacgctg atatttaggc acttttggtg ctttaatcgc cttaataacc
     5581 cacaccaccg caatcgccag tagtaaccac ggcagcaact taatcatcaa tgccagcata
     5641 ccgccgagga acataatggc cgtcgccaca accagcgcgg cgataatgcc cagcaacgaa
     5701 acgccggtga ccatcagcat gacaaaaaag ccaatcacaa aaagtagttc cagcatgatg
     5761 ctctcccaaa tatgaaatct cttgctggca ttacaagaat catgccaaaa ataatctatt
     5821 gatttaacag caaaacgccc cgcgacggtg cgcagggcgt ggtgaatttg actacttttt
     5881 ggtgaaaagt taacgcttat ccgccaccag tttgagcgcg tgttccagca cattaatgtc
     5941 tgcacccgct ttatgggcat tttcacttaa ataacgccgc cactgccgcg cgccaggaat
     6001 accctggaac aagcccaaca tatgccgggt aatatggccg agatacgtcc cctggctgag
     6061 ttcacgctca atgtacggat acatggcgcg cactaccgcc accggatcgg catcggtatc
     6121 cgaggaacca aagatctccc ggtctaccgc cgccagaata cccggattct gatacgcctc
     6181 gcgcccgacc atcacgccat ccatatgttg caggtgtgct ttggcctctt ccagcgactt
     6241 gataccaccg ttaatcgaca ttgtcagatg cggaaagtca cgcttcagtt gatacacacg
     6301 cggataatcg agcggcggga tttcacggtt ttctttcgga cttaaccccg aaagccaggc
     6361 tttacgtgcg tggatgataa acatctcaca ctcgcctttg ccggaaacgg tgttgatgaa
     6421 atcgcagaga aattcatagc tgtcctggtc atcgatgcca atacgcgttt tcaccgtcac
     6481 tggaatcgac accacatcgc gcatcgcttt cacgcagtcg gcaaccagct gcgtattacc
     6541 catcagacac gcaccaaaca tgccgttctg cacccggtca gacgggcagc cgacattcag
     6601 gttgatctca tcatatccac gcgcttctgc cagcttcgca cactgtgcca gcgccgccgg
     6661 atcgctaccg ccgagttgca acgctaccgg atgttcttct tcactgtacg ccaggtaatc
     6721 acctttaccg tgaataatcg cccctgtggt caccatttcg gtatacagca acgtattgcg
     6781 ggaaagcaga cgcaagaaat agcggcaatg tctgtccgtc cagtcgagca ttggtgcaac
     6841 gctaaaccga ccactccagt gaacgtcagt tttttcaggc attgcgctgg tttggttgat
     6901 tttttgcatt tcagaattac cgtgcatttt caaatgtaga gattatttta tcgatatatc
     6961 atggggttat gttattcagc atcactattc aggaggctca atagcggggt actataccat
     7021 aacaacagga agcgcctgtc tcattgcaaa agaaaattga gatcctctca aggcatgaag
     7081 ctctcacgaa gtgatggaaa taatcttatt agccgttaac tttgttaagg ccaatgataa
     7141 acaatccagg ttcgacgata aataaaaaat cacacattaa actctggtga tatatctccc
     7201 tgctaatagc attgatagag aaaaaagaac ccaataaata ttgggtcctt ttatataatg
     7261 ccttccatac tatcgaagaa cttcacatat tatttctccg attta
//
LOCUS       DAZGLJ010000222         7302 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000222 DAZGLJ010000000
VERSION     DAZGLJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.222"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(206..1306)
                     /gene="chaA"
                     /locus_tag="ACHK49_004112"
     CDS             complement(206..1306)
                     /gene="chaA"
                     /locus_tag="ACHK49_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO9264650.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1576..1806
                     /gene="chaB"
                     /locus_tag="ACHK49_004113"
     CDS             1576..1806
                     /gene="chaB"
                     /locus_tag="ACHK49_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO9264651.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            1964..2659
                     /gene="chaC"
                     /locus_tag="ACHK49_004114"
     CDS             1964..2659
                     /gene="chaC"
                     /locus_tag="ACHK49_004114"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO9264652.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2703..3056)
                     /gene="ychN"
                     /locus_tag="ACHK49_004115"
     CDS             complement(2703..3056)
                     /gene="ychN"
                     /locus_tag="ACHK49_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO9264653.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3241..4635
                     /gene="ychO"
                     /locus_tag="ACHK49_004116"
                     /gene_synonym="iatA"
     CDS             3241..4635
                     /gene="ychO"
                     /locus_tag="ACHK49_004116"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO9264654.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4636..5286)
                     /gene="narL"
                     /locus_tag="ACHK49_004117"
     CDS             complement(4636..5286)
                     /gene="narL"
                     /locus_tag="ACHK49_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO9264655.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5279..7075)
                     /gene="narX"
                     /locus_tag="ACHK49_004118"
     CDS             complement(5279..7075)
                     /gene="narX"
                     /locus_tag="ACHK49_004118"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO9264656.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7101..>7302
                     /locus_tag="ACHK49_004119"
     CDS             7101..>7302
                     /locus_tag="ACHK49_004119"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264657.1"
                     /translation="MSNNLNECDDTFWNGSILGYWLKYTHTWKELLLICRVVSRFTSL
                     RVGILQHREKSHNFYEITVLTMG"
BASE COUNT         1872 a         1776 c         1860 g         1794 t
ORIGIN      
        1 cgcaattcat cttcacttca tccctgaaat gtttgcaata aagagtacat tccggctttt
       61 caacagctgt tgcagtggtt tcgtgcaggt tgtggtacag gctcgcaatt ctgattaacg
      121 agctgtccag gtagtatgaa gaaatacaat aagcaggaat ttatcttatc tcccccgatt
      181 tccgtcatcg gaggagatat aactgtcagg caaatatcgt catcaaatag gcggcaaaca
      241 gtgccagatg cgctgcgcca ttgagcacgt tagtacgtcc ggtggagaag gagatatggc
      301 acagcactaa agaggccacc atcaccacca tttctggcgc accaagtgca aactgtaatt
      361 cgttacccgt cataaaggca attagcgtga cgacaggtac ggtaagcgaa atggttgcta
      421 acactgaacc aaagaacaga ttcatcgcgc gctggacctg gttgttcaac actgctttta
      481 acgcacctaa accttccggc gacagaatca acagagccac caggaagcca gtaaaggcga
      541 caggggcatt catgctgtcg agcaatgtct ccagcgggct ggcattcatt ttggtgactg
      601 caataacggc aatcagatgg ataatcaacc agatagcatg ccacacgctg ctatgggcag
      661 acggtttacc gtgatgcggg tcatcgtcat cactgtcatc ttcgtgctcg tagacaaaca
      721 aactttgatg cgttttggtc tggatcagca aaaatacgcc atacattgcc gcagaaatta
      781 atgctaccag taacgcctga ccggttgaaa aattcgccgc aggcagagcc attggaaata
      841 ccagtacgat tatcgccagg gggaacagcg caattaaata ctgcttgata ccaaacagat
      901 tcatatattg ggtggcaaac ttacgaccgc ccaacaataa tgaaaagcca accagcccac
      961 cggtaacaat cataatgatt gaatagagcg tatcacgcat tagcgttggc gcggcgtcgc
     1021 cggttgccat taaagctgaa atcaaactga cttcaagaat aaccactgaa aggctaagaa
     1081 taagcgaacc gtaaggttct cccaggcgat gggctaatac gtccgcatga cggacaacac
     1141 taaaggcgct acttaaaata ccaataagcg caagaagatt gatggcaatg accactggta
     1201 gtgtctggct gcttccccac aggaacagca ctaccagcgc cagaaccggg aaaataagcg
     1261 aagtctcctt gtggcgggtt tttaccgcct cttgagcatt tgacattatg gttatccctt
     1321 tgcagatgaa tttatcgaaa atgtaaaaaa taggtaggaa aaataacaga aagtgtctgg
     1381 atatcggtaa cattttacga atttttaccc cactgtcgat tttttactca ttgggacata
     1441 aaaataagta ttacgtttag acaatgtttg ttttagcgtt tcttaagaac agtctgacat
     1501 gaaaattctt atgttttggc aagagtagat attgttgacc acacttaatg ttcaactttg
     1561 taaaaggagt caacgatgcc gtataaaacg aaaagcgatc tgccggaaag cgtaaagcac
     1621 gttctaccgt ctcatgccca ggatatctat aaagaagcgt tcaacagcgc atgggatcaa
     1681 tataaagata aagacgatcg gcgtgatgac gccagtcgcg aagaaaccgc gcataaagtg
     1741 gcctgggctg ctgtgaagca tgaatatgcc aaaggggatg atgataaatg gcataaaaaa
     1801 tcgtaaaacc ggtagcttag ttaaagctat tcgtgcggtg ttgccttgca agtggtccgt
     1861 ggattgcata ttgtcccgtt agtggtttca aaatgagcag taaaaatgtc cggaagacac
     1921 caaaaagttg tcgcagggaa gtatgcagtg gcggaggtgt aaggtgataa cgcgtgattt
     1981 cttgatgaat gccgattgta aaacggcatt tggtgccatt gaagaatcac tcttatggtc
     2041 agcagagcaa cgggcggctt cgctggcggc gacgctggct tgtcgacctg atgagggacc
     2101 ggtgtggatc ttcggttatg gatcgttgat gtggaatccg gcactggagt ttaccgagtc
     2161 gtgcaccggt acactggttg gatggcatcg cgcgttttgc ctgcgcctga ccgccgggcg
     2221 cgggactgcg caccagccgg gacggatgct tgcactgaaa gagggcggac gcactacagg
     2281 cgtcgcctat cgactgccag aagagacgct ggagcaggaa ctaaccctgt tgtggaagcg
     2341 cgagatgatt accggctgtt atctgccaac ctggtgtcag cttgatcttg atgatggatg
     2401 cacagtaaac gccattgtgt ttattatgga cccgcgacat ccagaatatg aatctgacac
     2461 tcgcgctcag gtcatcgcgc cgttgattgc ggcggcgagc ggtccgctgg gaaccaacgc
     2521 acaatacctg ttttcactgg aacaggagct tatcaaactg ggaatgcagg acgatgggct
     2581 gaatgatttg ctggtatcgg taaaaaaact gctggcggag aattatccgg atggtgtgtt
     2641 acggccggga ttcgcctgag taaacttccc tcatagtggg gcgtcagacg ccccctcaaa
     2701 cattaaaatg tgagcacttt atcggctgac agcgtccatt gcgccagttc cacaagagta
     2761 ccgatttcca ccccatcaat cagaggaagt gtactaatcc cgcgcccgtc ggtacaggtt
     2821 ttgcacaatt tcaccggtac attctgagcg gtaaggatct ccagcatttg ctgaatgttg
     2881 tagccttccc ctggtttttg cccgcgcaac ccggctgtga ccgcatcaga catcaggaac
     2941 agacgcagat ccagattgct ctcctgctct cgtaacgcaa tggctagccg caagctgtta
     3001 aacaaggatt cgctcccgta aggtgcgcca ttggcaacga tcacgatttt ttgcattatt
     3061 tactcctgta ttcagggaat tagacattca tcttctatct tactgcttct gcagcgtctg
     3121 accaatcggt cacattttta aggattttcc tgaaagcgcg agaaaatacg acaaaagttg
     3181 ccagtaatcg ttattcttta aggctatggt ttttcattat taccggaagt taccgacgtt
     3241 ttgagccgtt tcgttcctcg cattatcccg ttttatttac tcttgcttgt agcaggcggt
     3301 acagctaacg cacaatctac cttcgagcaa aaagcggcaa atccctttga taataacaat
     3361 gatggtctgc cggatttagg catggctccc gaaaatcatg atggggaaaa acactttgct
     3421 gaaattgtga aagatttcgg cgaaaccagt atgaatgata acgggctgga tactggcgag
     3481 caggcaaaag ctttcgcatt gggaaaagtc cgcgacgcgc ttagtcaaca ggttaatcag
     3541 cacgtagagt cctggctatc accgtgggga aatgccagtg ttgacgtcaa agtggataac
     3601 gaaggacatt tcaccggcag tcgtggaagc tggtttgtgc cgttacaaga taatgatcgt
     3661 tatctcacct ggagccagct tggtcttact cagcaggatg atgggttggt gagcaatgtg
     3721 ggcgttgggc aacgctgggc gcgcggcaac tggctggtgg gttataacac tttttatgac
     3781 aacttgctgg acgaaaatct tcagcgagcg ggctttggtg ccgaagcgtg gggcgaatat
     3841 ttgcgactat cggcaaactt ttatcagccg tttgctgcat ggcatgaaca gacagccacg
     3901 caggaacaac ggatggcgcg cgggtatgac ctgacagccc ggatgcgcat gccgttctat
     3961 caacacctca ataccagtgt cagcgtagaa cagtattttg gtgatcgtgt tgatttgttt
     4021 aactctggta cgggttatca caatcccgtc gcgttgagtc tgggattaaa ttacacccct
     4081 gtgccattag tcactgtgac ggcccagcat aaacagggtg aaagtggcga gaatcaaaat
     4141 aacctcgggc tgaatcttaa ttaccgcttt ggtgtaccgc tcaaaaaaca actttctgcg
     4201 ggtgaggttg ccgaaagtca gtcgttacgt ggtagtcgct atgacaatcc gcagcgaaat
     4261 aatctaccga ctcttgagta ccgacagcga aaaacgttaa cggtgtttct ggcgacaccg
     4321 ccgtgggatc taaaacctgg cgaaacagtg ccgctgaaat tacaaatccg cagtcgttac
     4381 ggtattcggc aactgatttg gcagggcgat acgcagatat taagtttgac gccgggcgca
     4441 caagccaaca gcgcggaggg ctggacgctg atcatgcctg actggcagaa cggggaaggg
     4501 gcaagcaatc actggcgatt gtcggtggtg gtggaagata accaggggca gcgtgtctcc
     4561 tccaatgaga tcacgctaac gcttgtcgaa ccgttcgatg cattgtcaaa cgacgaactg
     4621 cgctgggaac cgtaatcaga aaatgcgctc ctgatgcacc cataccgctg cttccacgcg
     4681 agacttgagc ttcattttct tcagcatgtg cttgacgtgc acttttactg tgctttcggt
     4741 gatatccagg cggcgggcaa tcatcttgtt cggcaaaccc tgggcaatca gcttgagaat
     4801 atcgcgctcg cgtggggtta actggttaac atcgcgctca gtagtggcac ggttagcgcg
     4861 caagctggcg gccagaacag gcgttaatgc ttcgcttaat accatttcgc cagcagctgc
     4921 ctgatgcaat gctttcagca gatcttccgg ttccatatct tttaacagat aaccatccgc
     4981 gccgcgtttc agtgcggtga ccacatcttc ttcatggtta gagacgctga ataccacaat
     5041 gcgccctgag agggactttt cgcgcagttt atccagcgtt tccagaccgt tcatgccggg
     5101 catattgaga tctaacagga tcagatcggg atcaagagac tccgccagtt caataccctg
     5161 ttcgccatta ctcgcttcgc caaccacggt gatatctggt gccatactga taagctgttt
     5221 tacgccagtt cgcagcatcg ggtgatcgtc aatcagcagg atagtagccg gttcctgatt
     5281 actcatgggt atctccttgg acgtctgtga aagttttttc gggaataaag gtgaccacca
     5341 cttcggtgcc acctgattca cgacggcgga cgcggcaatc gcctcgtaaa ctttgcgcac
     5401 ggtcgcgcat tattatcatg ccgtagtgat tgctgcggat ggcattttca ggcacgccgc
     5461 agccgttatc ctggacggtc agtttgacct gattatcgtt ttgcgccacc gtcaccacga
     5521 cttcactcgc ttgcgagtgt ttgagggcgt tacttaatgc ctcacgggca atttgcaaca
     5581 agtggattgc ctgatgcgaa ggcaccaggc gaggcggcaa ttgataatcc agcttcaccg
     5641 ggaagccaaa tttggcgctg tactcttcgc aactcgcctc cagcgccgga cgtaatccag
     5701 gctcggtgag ctgcaagcgg aatgtggtga gcaattcacg caactgcgcc caggatgcat
     5761 tcagttcgtt acggatctga cttaacagtt cgcggctgct ttctggcagc gcatcgccct
     5821 gcatctgtaa acaactcacc tgcatcttca tgcaagagag agattgggca atagaatcat
     5881 gcagttcgcg cgcaatggtg gcacgctctt ccatcacgat caactgttgc tgacgttcct
     5941 gatggcgatc cagcgccagc gtggcggtga gttgttcaac cagggtatcc accagttgtt
     6001 gttgatcatg gctaagatga cgcccctgcg gcagggtcgc cagcaaaata ccgtactgcg
     6061 tatgagagtc agccagccgc cacttcaggg ttgtgccgcg atcaccaacg ggtaatacgc
     6121 cgcgcgggca gagctggcag cctttatcat cacaagtcat atctggttgg caggtaaact
     6181 cctgatgatt ctcttcatca tcagtatcat aaacccgcag ttcgatatca cgtagcaggg
     6241 ttaaattctg taagccgttg agtacaggtg acaggcgttc acacagcggg gcttgggaat
     6301 gcaaacggcg gttagcctgc cataaaaaag agaggatctg atttttatgc tccagcccgg
     6361 cggttttctc ctgaacccgc tgctcaagta cggcataact ttcggccagt tctgcagaca
     6421 tattgttcaa cgcagttcca agcatcgcca tttcgttgcg cccgctgatg tttgcgcgtt
     6481 gggtaaaatc gcgatgactg acggcactcg ccattgccag cagttgccgc cacggttgta
     6541 gcagtcgcgc ccgcaaccag ataatagtga acaccagtaa aagtgccata aataccgcca
     6601 ttacccgatg gaccagtacc actgtctcga tgcgcatttc cgtggtgcgg tcaaaaccag
     6661 ataccagttg atcaagcccg gcaacaaact ggctgacatc cgctgacacc gtttctcggt
     6721 tttgtgcacg catcaacgca gggatcagtt cattacgcca gtaatcttgt aaaccctgta
     6781 attgcgccag ttgtccgtct cgttctgctg ctcgagtcaa ctcggcgcta aatgccgttt
     6841 gttccatctc tttaattaag ggcttgtctt tctcgcttaa tggcactgct gccaacagac
     6901 ggtaactttg catgcgcagc gatcccgctt tgttgatcgc atgggcgctg ccctgaacgc
     6961 cttgcaccag ccagccagaa accgccatcc ctgccagtcc aatagcagta gaaagcaaca
     7021 caataagcgc aacctgatta accagggtga gcggagagag acaacgttta agcatgtaaa
     7081 cctcttcctt caggctttaa atgagcaata accttaatga atgtgacgat acattctgga
     7141 atggcagtat tctcggctat tggctgaagt atacccatac ctggaaagag ttactcctta
     7201 tttgccgtgt ggttagtcgc tttacatcgt taagggtagg gattctacag caccgtgaaa
     7261 aatctcataa tttttatgaa atcactgtac tcactatggg ta
//
LOCUS       DAZGLJ010000223         7242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000223 DAZGLJ010000000
VERSION     DAZGLJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.223"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..114)
                     /locus_tag="ACHK49_004120"
     CDS             complement(<1..114)
                     /locus_tag="ACHK49_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264658.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNG"
     gene            complement(239..2239)
                     /gene="yehQ"
                     /locus_tag="ACHK49_004121"
     CDS             complement(239..2239)
                     /gene="yehQ"
                     /locus_tag="ACHK49_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO9264659.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEGLATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRALQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2236..3372)
                     /gene="yehP"
                     /locus_tag="ACHK49_004122"
     CDS             complement(2236..3372)
                     /gene="yehP"
                     /locus_tag="ACHK49_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO9264660.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3365..5644)
                     /gene="yehM"
                     /locus_tag="ACHK49_004123"
     CDS             complement(3365..5644)
                     /gene="yehM"
                     /locus_tag="ACHK49_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO9264661.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIKGPADFNDRV
                     DELFLSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEANMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            complement(5655..6743)
                     /gene="yehL"
                     /locus_tag="ACHK49_004124"
     CDS             complement(5655..6743)
                     /gene="yehL"
                     /locus_tag="ACHK49_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO9264662.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1694 a         1957 c         1938 g         1653 t
ORIGIN      
        1 gccgttcaga ttggcgctga atttcttcga ttcttctttg tcaccgcagc cagcaagaga
       61 gaaaaccaga acagatgcaa caacgaggga aaacagctta ttgaaagcct tcatgtaaaa
      121 ctccatttta tttaatcaag aaactggtga ctctcaccag gggctatata gaatatgcct
      181 aataccgtga cgtgagcagt ccggaactgg agtagaactc ttagtaaaaa gcactatttc
      241 atccttgttg ctgaagcatg gggaataatt gttcgcaaag taaaacaccg ttattcattg
      301 cttctacccg tgcctcgctt tctgtattac gaaattgtcc caacacatgt gccagccgat
      361 aaaaaccgac cgcggagagg tcattcgcca gcaactctgc ctgactaata gcgctctgtt
      421 cctgatagcg ccagccgtta tggagcagtt gaataagtaa cgcctggcag cgcatcagca
      481 actgatgagc ggtagacggc acaggcaaaa cgctggcaga aggtagcggt gccacaggcg
      541 cagtttccgc gtccagcgcc caggcgcggg tttttgtcat catcacccgt ggttccagtg
      601 tcaattgccc atcaacaaaa ctgacaaagc cagaaaccag actcacgggg tcgtctgttt
      661 gttgcaaaag cgccgccatg cgttcaacgg caaaaggaga acaggcagat gccggaaggg
      721 ataacgtcag gagattatcc tcaccttcgc cgctgattac ctgcgcatcc agcgtctggc
      781 ggctgctatc ccaaccgagc gaaatacact cagcgaccgg cagaataaat aagttatcga
      841 cctgattaag aggccgtatg caggcggggg gacgctggcg taaatattcc cgcaaagcca
      901 caatgcccgg ctggcgtaac ggcgcgctca acatttgcca ggcatcaggc gacagcggca
      961 caacgctgct taagcggttg cgggtagcta acagcagctc gccatcggca ctgcgttttg
     1021 ctgcttgtga aacaatttgc ccacccgcca gtgcgccagc ctgaaaacta aacagccgac
     1081 gcgtagctgc cggtgagttt tcctgttcac ttcgcggcca actgcgcgaa aggtgcaaaa
     1141 tactgccggt gtcgggatcg gtaaaccaga tgcgtaaacc ataatgctca atatcctgcc
     1201 agcaacgcat acctaaagac accagccgca gatgatcaag ctttgcttct ccggcaatgc
     1261 cagagccaac gaccgtgcgc cacggcacag gaggaacttc accaacactg tcgcgccggg
     1321 ccatctcttg tgcgcaattt aatcgactgt ttaatgccgc aagctgacgt aagcattctc
     1381 cggcatgata gtggctggcg cgagtgtgga aggcatcaac gcttgcccgt agctgtcgta
     1441 gtgattcact cacccagcgc cagttgcagg cctctgccgc ctgcaatgcg cggttgaatg
     1501 ctgcctcgta gtgaataagc ggctggctga tgccgccaag ccataatgcc tggcttaatt
     1561 gctgaacata ttgacgacac gtttggcctt cttcgctggc aaacggatcg tcagatgatg
     1621 tgacgtgttc gctgcgcatc tgccagatta aatggttaaa ttctgcttgc tgcgctttgg
     1681 cctcgacgaa ggcctgcaca gccagtacga catgttcgca aagtgtgcct tcaatacaat
     1741 cacaacgggc gaaacgaata ctgctacggg aataaaaacg cacatcactc atcggtaagc
     1801 gggcagaggg aatttcgccc ggcgcacaga acaactcaat ggtgatgcct ttagcgacca
     1861 gcgcctgcgc gcgtttgcgg gtggcatcgg gcagggtagc cagtccttcc agccagattg
     1921 ccggatccca ctcttcttct ttttccgtgg gctggacggt ggcacaaagt cgttgataac
     1981 ttaacaccag catcacgcga tgacggcaca tgccgctggc cccgcagctg cactgagcct
     2041 ctttcagtgc ctggctgttc gccagctggg tacggacacc gtcactgaag gtggcgatta
     2101 aagcgccgtt ctcatggctg atttccggga cgttgccatt ttccagttcc ttaagactgc
     2161 gcttaacaaa accggcattg cttaacgccg tcagtgcctg cggtgtcagt tctaataatt
     2221 ccggacgtag tgaattcatg actgaagatt ctccgcaagc catgatgcca gctcgcccgg
     2281 cgtcatggcg gctatttgtg cgccgacatt aaccagcgcc tgggccgtat cgcggtcata
     2341 gcaaggcgtt gcggtgctat cgagcgctgc cagtcccagc actttgatgc cgctctggac
     2401 acactttttc acctgatgcg tcagcaatga tgatgaaccc ccttcgtaaa aatcgctcac
     2461 gaggataatg acgctttttg ctggttgttc aataagttgc cgaccatact ccacggcact
     2521 ggcgatattg gtcccgccgc ccaactgtac tttcattaat aactctaccg gatcggcaac
     2581 gtctgccgtg agatcaacca cgcttgtgtc aaacgccacc agatgggtac gaatgccggg
     2641 taactgccac aaacaggccg ccatcaccgc agagtggatc accgagtcca ccatcgatcc
     2701 gctttgatca accagtaaga ccagttgcca ttgttcgctt tggcgtttaa tgcggctgtt
     2761 aaagcggggg gattcgatat acaacttgcc gtgttgcggg tgccagtgtt gcaggttggc
     2821 gcgcagagta cttttgaaat caaagtttcg cgccagtgga attaatgagc ggcgacggcg
     2881 atcgcggaca ccagaaaaag cctgacgaac ttcctttgcc agtcgcgcca taatttcttc
     2941 aacaacctgg cgcactatct ggcgggcggc agccagtact tcgggattca tcagatgttt
     3001 ggtgtgcaaa acggcgcgta gcaggctttc cgaaggctgc atacgttcca gcacgtcgag
     3061 attcgtcacc acatcttcaa tgccgtagcg cagtacggca tcgctttcca gtcgctcaat
     3121 cacctgttgc ggaaacagag tgtgaatact gttgatccac tcaggggtgg tgagatttga
     3181 gccacctaat ccaccagagc gttcaccacg ctggagccgt tcaggatcgc gcccatacag
     3241 ccactccagc gcgtggtcta tctgccgggc gttgtcatcc agcccacaaa gcgtcgtttc
     3301 tgccgcttcg ccaagaatta atcgccagcg ttgtagctca cgggtggtca gaagatcgtt
     3361 cagttcagac atggaaaact ccccagtttt gcagcgatgc cagtgcctgc tgttcgagtt
     3421 gttgatgatg tgcaatggcc tgtggtggac aatgcaacgg catttgcagc gccgaaacgg
     3481 gaagttgcgc cagttgataa tgctcaagca cctgatgcgc cagcgtcccg cgttctcgtg
     3541 gtggtagcca ggccatcgcc gcgcgtaaat cgggcagggc attgataaaa tcggcatcac
     3601 ttagttgatt taaatgactg ctgaaaccgg cgataaatgt cggctgacag gccagttgat
     3661 ggcgggccag cgccagcaaa ccgtgcagcg cttcgccgga ttgtgccggg gataactgcg
     3721 ccagcatcgt cagcgccgct tcggcactgg cgttaggatg ttccagacgc atcagtgcgc
     3781 catgagccgc gccgcgatcc agcgccgggg catgaattgc ctgactgcgt cgctccagta
     3841 aggctaccgc cgcagaaaga gaaatgccgg ggagttgcac accgctatgt agatcgcgca
     3901 ggatatgaca caacgcttgc cagctatgca ggtgagcgtg aaactccttt tcatctggtc
     3961 tgccgttaga ttcacatagc cacagcgtgc gatcgatagc cgcgcataac gtcgtttgta
     4021 atatctgcgc gccttgcata ccgctaattt catccagccg ccataaggca tatagcactt
     4081 ccagcgcctg gcccatttcg gcaaattgat tttcctgggc gattaataat gtgagttgct
     4141 ccagtaattg ttgactgaag gacgccagac ccgctaacgc cgcctggcta agacatgtgg
     4201 tgatactgcc gattccaccc gcgtccagca tattggcttc taatttatgg cgtgcggctt
     4261 cctggagtgt tgcaccaaag caggcggcct caatcaatgc cgcatgctgg ctaagcgggc
     4321 gggttaattt ccagcgttct tcaccgttgc ctgcaagtgt cagtgtgctt ccctgctggc
     4381 gtacaatccc tgggatctcc agtattgcca gccgatgtaa cacctgactt tgcgctagtc
     4441 cattggggtt aaagcgattt aaggtaagct ccgccggaag agagattgcg gtacgttcca
     4501 gttcgcaggt gacatctttt ggcagaggcg gttgtggcgt agaaggggca agtttaccga
     4561 atccgtcacc cgctaatgtg tctatcaacg ttagcagaat cggatcggta tcgggatgaa
     4621 taacgccgcg atagctccac ggcaacggcg cattcagggc ttctttaatc agcgagcctg
     4681 ctatggcatc cagccagtca gtgcgtaacg gtagtgtatg accgcgcaac tgtgccagcg
     4741 ccatcgcatg cagatgagcg gcagccatat ccgcggtcga agcaggcaac ttgtgctggc
     4801 gcaaacgggt aaggatagtt tttagcagtt gttcaccggc ctgctgtaag ccccactgcc
     4861 agcaccagtt ttgccagacc ggggcaggca taccggaaag gtatcctgcc agcacatcaa
     4921 ggcgcttttc actgtagggc gtgagataac aacctgtaat ggcatctgcc agcgagggca
     4981 attccggcgt gttaatgtcc tgcgggcatt cgcgccacat ttttgccagt gccggagcgt
     5041 gccagccacc gcagacgact aacacgtcgc cattattttg ctgcaccgcc catgcaatcc
     5101 agcgagccat aaaggcttcg cgctgacgat tgagtgcatc gccggggaaa tcgccccgca
     5161 gttgggcaaa atagtgcgcc agcgcagagg gtaatgcagt ttgctggctt tcatcttcga
     5221 acaagtgatc ccacagggta tcgctgttat ccatgcgggt ggcacgcagc agtaaggcct
     5281 ggctttcatc ttgcgtatca ggcgaatcgt cctcttcttc actctgcgcc cagcaaggca
     5341 aatcgatgaa gtaagtttgt gcctgaatac gacgtgcggc ttgtagcgcc tgccactccg
     5401 gcgaaaattc agcaaatggc gtccaggcac cacgcccggg ggctgcaccg tcctgatact
     5461 ggcaataact gtaaatagct accggaagct ggtgggataa aaacagttcg tctacccggt
     5521 cattaaaatc agccgggcct ttaatcaaca cgtatcgtgg ccgctggctt tcgattaacg
     5581 atttcaccag ccgggcgcag gccggactat gatgccggat gccgacaatt aacggctcgc
     5641 tcatgcatct ttcctcacgg caggcggtgg cgggcttgat aataagcctg ccagtgagct
     5701 tctttatgtg tggcgactcg ttgttcgaag taacggcgca gacgagcgcg atcttcttcg
     5761 ttatctttga cgatggttcc ggcaatgcag tccaccaggt cggctggctc acctgcgcga
     5821 ttcgctaaaa accaggcgcg gacgcctacg gcgtgggcca cgttcacggc ttcagcggtg
     5881 gacataatcg ctgttagtgt atccattgac gttttctttt cgccgttggc acgcagatcg
     5941 cggaaggtgc ggaccagtaa ctccagtacc gcgtcaggca ctttatgcgg aataccgcta
     6001 tgggccagca aacgcgccga agcactggcg acaagctcca gctcctgagc gaaatccata
     6061 atcggaaaga cagtttcaaa atcgaagcga cgctttagcg cggcactcat ctcattgacg
     6121 ccgcgatcgc gggtattggc agtggcaata atattaaagc cttcccgcgc atagagttga
     6181 ctggcttcac cagtgagttc cggcaccgtc ataactctgt cagaaagcat acctaacaga
     6241 caatcttgca cttccagcgg tgtacgcgtt atctcttcga aacggacgat tttgccgtcg
     6301 cgcattccct ggtaaagcgg ggcagggacc agcgcttccg ttgatgggcc atgattgata
     6361 agcagagcgt agttccagcc gtacttgatt tgatcttcag tggtagatgc gccgccctgg
     6421 atggttaacc cggcatcgcc gctaatagcg gttgccagta attcagacag gagagatttt
     6481 gcggtgccag gttcccccac caacatcaaa ccgcgtccgg tggcaagagt gactaacata
     6541 cgttcgacag cggagggact aatcaccact ttacggctaa tattttgcgc gctatcgcca
     6601 aggataaaag cacgggccgc aggcaaactt aactgccaac cgggcgggca aggtgcgtta
     6661 tcattttgtt ttaattttgc cagttcatcg gcgtataaca cagcagcagg cggacgctgc
     6721 agatgattat tctgcggtga catatattca tccctgaaat gtattaatga aataaacgat
     6781 attttacgcg caataaatgt ttggcgcatt agttgtttga gcggaaaata tctggaaagc
     6841 agatcacagt aaataaaaaa acctgctggt gaggcaggtt cttttattta ctattttcag
     6901 gcctaacacc acagcctgtg caacatcgtg ttccaacgaa cgaattataa ccacggctgg
     6961 accccgttgc aattaagata atgagattta taatcctgac tgttgggacg ttgcgccgga
     7021 tcgaacctat ttcaagtcct ggctaccgga cggggggtgc tgcaggtggt gccctgatcc
     7081 ggagtgcaac atcgtggtcc aacatacgta aaccagtatc gacaggcagt gtaagggtaa
     7141 ggtgctggtg ttgcgctcct taataaggag agcggatgga ctcgctgaca caaaagttaa
     7201 ccgtgctcat tgctgtactg gagctattag tggctctgtt ac
//
LOCUS       DAZGLJ010000224         7214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000224 DAZGLJ010000000
VERSION     DAZGLJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.224"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(60..1652)
                     /locus_tag="ACHK49_004125"
     CDS             complement(60..1652)
                     /locus_tag="ACHK49_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO9264663.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHK49_004126"
     CDS             1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHK49_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO9264664.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2850..3968)
                     /locus_tag="ACHK49_004127"
     CDS             complement(2850..3968)
                     /locus_tag="ACHK49_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO9264665.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHK49_004128"
     CDS             complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHK49_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO9264666.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4618..4746
                     /gene="mntS"
                     /locus_tag="ACHK49_004129"
     CDS             4618..4746
                     /gene="mntS"
                     /locus_tag="ACHK49_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO9264667.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5018..6601
                     /gene="opgE"
                     /locus_tag="ACHK49_004130"
     CDS             5018..6601
                     /gene="opgE"
                     /locus_tag="ACHK49_004130"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO9264668.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHK49_004131"
     CDS             complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHK49_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO9264669.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
BASE COUNT         1821 a         1946 c         1709 g         1738 t
ORIGIN      
        1 attttgcatt tgtcatcagc cgccgtcacc ccgacagtat cggggtgacg gataatcact
       61 tactcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcacgcgt
      121 tccggggtga tttccagaat gcgggtcgcc agggagctta cgaactcacg gtcgtgtgaa
      181 acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagtgactc gatggattcc
      241 atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac
      301 ttaccaaaca gcatccgccc tttttcccca ccggaaagca ctttagctgg ctttttgatg
      361 tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc gtcatcgcct
      421 tcctgcttcc actgactcat ccattcgaac acggtcagat cattttcaaa ctcatattcg
      481 tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc attttacggt gccgctgtcc
      541 ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc
      601 agtaccgcaa gtttttcacc gacctccagc agcaggttga gatttttaaa cagtggaccg
      661 ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag tttcttatcc
      721 tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt tcacctcttc cagtttgatt
      781 ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag
      841 cggctaacga aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt atcggccagc
      901 agacgttcac gcgcctgggt cgccgccgtc atgtactcat cgtagttacc cggataaacg
      961 cgcagctcgc cgtaatccag atccgccatg tgggtacaga ccatgttaag gaagtgacgg
     1021 tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc cagccagcga
     1081 atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca ggagaatatc cggatcagca
     1141 aacagcgcct gcgccagaag cacacgcagc ttccagccag gagcaacttc actcatcggg
     1201 ccgtagtgct gttccactgg aattcccacg ccaagcaaca attcaccggc gcgagcttcc
     1261 gcagagtaac cgtccatttc gccgtattta acttccagat cggccacttt atagccgtct
     1321 tcctcactca tttccggcaa agcatagatg cggtcgcgct cctgcttcac ttcccacaac
     1381 tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc
     1441 tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccaa agtcggctct
     1501 aagtcgccgc cgaggatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg
     1561 ccgtaacggt tgccgccgcc aaatttgacg gaaatgtttt caaacaacgg cttactgccg
     1621 aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa
     1681 accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc
     1741 aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa
     1801 cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat
     1861 ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg
     1921 cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt
     1981 tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc
     2041 cgcggagtac cagatggggc tttccaatat gatggaagcg aacccgggtg tggatacctt
     2101 cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt
     2161 tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac
     2221 caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagata cgcctatcaa
     2281 ctggaccacc aaagttgagc gtaagaaagc aggcccgacc tggacgccga ccgccaaaat
     2341 gcacgcagag taccgcgctg cgggcgaacc gcttccggct gtcgttccgg caggtccgga
     2401 taacccgatg ggtctgtatg cactctacat cggtcgcctg tatgctatcc atggcaccaa
     2461 cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga
     2521 catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc
     2581 ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc
     2641 taccaccgaa gcccagtttg aaggtcagga aattgtgcca attaccctga cgaagagcgt
     2701 gcaaacagtg accggtcagc cagatgttga ccaggttgtt cttgatgaag cgattaaaaa
     2761 ccgctccggg atgccggttc gtctgaatta atcttcaaaa cttaaagcaa aaggcggact
     2821 cataatccgc cttttttatt tgccagaccc tagttggccg ggagtataac taacaaaaca
     2881 tatcccacca acgccgccca caacagcatc ggtattgaat agagatggaa acgccaccag
     2941 atgcggcgat cgttcgccat acgtagcgca atcaaatttg ccagcgatcc gggtaataac
     3001 ccaaagccac ctacgtttac cgcccatacc agtaataaag acggcggcac atagttcagc
     3061 aacaatatgg tactcggcac gttactgatc acctgcgata aaccgattgc cgttaaccat
     3121 aacccgggtt cagatagatg actcacgtta cccaacacgc cttgcaacgc tggaagctgg
     3181 gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcagcgt ccagtccaca
     3241 ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg ccacaatcac cagtccccac
     3301 agctcttgtt tgaactccag cgccgtcaga aagacgatat acagccccag acaactccac
     3361 accagccgcg gtttccactc cggtgtttgc acccccgtat ggtattgcag cgcctttcca
     3421 gggaaacaac accagcacag gagcaggagc gtcagcatca ttgcgccagc cagcggtgcc
     3481 atttgggcaa taaatccggc aaacgaaaga ccagaacgtc cccagataag aatattttgc
     3541 gggttgccaa ttggcgtcag tagcgaacca gcgttgactg ccagcgcctc aaaaataatc
     3601 agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg aacaacaata
     3661 aacagcgcga catcgttggt cagaaaggta gaaagcagcg ccgccgccag caccataaac
     3721 atcgccagcc gacgctccgt agcaaagcgg cgcaccattt tgcgccccag cacatcaaaa
     3781 taaccgctta actccacacc tttggtcagc agcatcaggc cgcttaaggt gatgatggtg
     3841 tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gcacgaaaaa gcttaatccg
     3901 ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt gcagcgtgcg taaaaaaggc
     3961 aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa agcgtcgagc gtctcttcac
     4021 taacatggtg ctccatgcct tccgcgtcgc gacgggcgat ttccggactg acgcccaaca
     4081 ccaacaagaa attttcgact atctgatgtc gctcgcggct ttcctgcgcc agcttctctc
     4141 cttctgccgt taaaaacacg ccacgccagg ggatcatttc aatcagcccc atggttgcca
     4201 gccgcttaag cattttagcc accgtcggtt gcgaaactcc cagacgagca gccatgtcca
     4261 cctgacgagc ttcccccact tccctgatca agtcagaaat cagctcaacg taatcatcaa
     4321 taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc ttcaacgtgc tcttccacgt
     4381 tcactaactg cgtcactttt tttgctgttg gcgtacctgc gcgacgactc attgtgcttc
     4441 ctcattatgg tgacgcatac atagcgttct catttcgtgc gcacattgta aaccagagtt
     4501 gcgaaggtac aaaaaattaa cgttttagca atagctatat aatatagcct gtgctatatc
     4561 tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta gcaagtcagg aggtcttatg
     4621 aatgagttca agaggtgtat gcgcgtgttt agtcattctc cctttaaagt acggttaatg
     4681 ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc agcaagataa accttccgat
     4741 aaatagcggc gtcgcggtac gccgcttcac tcctgctttc atgcaggcat aacgcgtttt
     4801 ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc ttcgcaaaag atttgttcgt
     4861 cagtagttga cctgaacggc ggctcgctct atcttcttgc agccctgcgt atattgcggc
     4921 tcgcggatgc ggaccccttt cccctcttca cgcactcttg caggtattga cccttgacgc
     4981 cagggtaagc acatggcgtt tgttacgata gtggcatatg aatttaaccc tcaaagaatc
     5041 gcttgttacc cgtagccggg tatttagccc gtggactgcg ttctactttt tacagtcgct
     5101 attaattaac ctcggcttag gttacccctt cagtttgctc tacaccgctg cgtttacggc
     5161 tattttgctt ttgctatggc gaacattgcc tcgcgtacaa aaagttctgg tcggtatcag
     5221 ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc tacggcgcgc ctaacttcaa
     5281 tacattgctg gcattgcact ccaccaatat ggaagagtcg accgaaatcc tgacgatttt
     5341 tccgtggtac agctacatgg tcggcatatt tatttttgtg ctcggcgtaa tagcaatcag
     5401 gcgaaaaaaa gagagtgaaa aagcgcgctg gaataccttc gacagcctgt gtctggtatt
     5461 cagtgtggcg acattttttg ttgctcccgt gcaaaacctg gcctggggtg gcgtatttaa
     5521 actgaaagat actggctatc cggtatttcg ttttgctaag gatgtcatcg tcaataataa
     5581 cgaggtgatt gaagagcaag aacggatggc aaaactttcc ggaatgaaag atacctggac
     5641 ggtcactgcc gttaagccga agtatcagac ctatgtggtg gtgatcggtg aaagcgcgcg
     5701 tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat accccgtttg ccagcagcgt
     5761 taacggtttg atatttgctg actacattgc cgccagtggc tccacgcaga aatcgcttgg
     5821 cttaacgctc aatcgcgtag tcgatggcaa accacagttt caggataact ttgtcaccct
     5881 ggcaaatcgc gcgggcttcc agacctggtg gttttccaac cagggccaaa tcggcgaata
     5941 cgatactgct atcgccagca tcgccaaacg tgcagatgaa gtgtacttcc tgaaagaagg
     6001 taattttgaa gcagataaaa acacgaaaga cgaagcgtta ctggatatga ccgctcaagt
     6061 gctggcgcaa gagcactcgc aaccgcagct gattgttctg catctgatgg gctcgcatcc
     6121 gcaggcctgc gacaggacac aaggcaaata cgaaaccttt gtgcaatcga aagaaacgtc
     6181 gtgctatctc tataccatga cgcaaacgga cgatttactg cgcaagctgt acgatcagtt
     6241 acgcaacagc ggcagcagct tctctctggt ttacttttct gaccacggtc tggcctttaa
     6301 agagcgcggc aaagacgtgc aataccttgc ccatgatgat aaatatcagc aaaatttcca
     6361 ggtgcctttt atggtcattt ccagcgacga taaagcgcat cgggtgatta aagcccgccg
     6421 ctcagccaat gacttcttag gctttttctc gcagtggacg ggaattaaag cgaaggaaat
     6481 aaatatcaaa tacccgttta tatctgagaa gaaagccggg ccgatataca tcaccaactt
     6541 ccagttacag aaggtggatt acaaccatct cggaaccgat attttcgacc cgaaacctta
     6601 aaacaaaaaa tccgccccga gaggcggatt ttttatatca ccaaagtgat tagaagcggt
     6661 aaccaacacc ggcaatccag gtacctacgt caacgctacg aatacggctc tgctcgtaag
     6721 agaagtccag agcaacgttt tccatcgggt tgaactgcag accagcaccg taggagaaac
     6781 cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattccgt ggtctggaat ttaccataac
     6841 ccacccccac tacaccgtag atgcttgccc agtcgttaat gcggtaagcc ggaccagcag
     6901 tgatgccgta gtactggttt ttgttgtagt caccagagct tgcagtacgg cttttctcgg
     6961 tgtaagtgaa agagccgatc acacccagcg ggctgttgtc ttcttcatag cggtatttca
     7021 ggttgaaacc gcccattttg ttcatttggc cctgagcgtc gctctgtgcg taaccgccag
     7081 ttacggtaga agtcgcagct acggaagtac ctgcggtgaa agccagaact gcggccagtg
     7141 ctgaaagaca tgcaattttt ttcataacca cctcaaatgt gattcaaata agtcctaagt
     7201 tttaaatata tcaa
//
LOCUS       DAZGLJ010000225         7210 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000225 DAZGLJ010000000
VERSION     DAZGLJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.225"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            420..1547
                     /locus_tag="ACHK49_004132"
     CDS             420..1547
                     /locus_tag="ACHK49_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFO9264670.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
     gene            1553..2344
                     /locus_tag="ACHK49_004133"
     CDS             1553..2344
                     /locus_tag="ACHK49_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFO9264671.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(2380..3305)
                     /locus_tag="ACHK49_004134"
                     /pseudo
     CDS             complement(2380..3305)
                     /locus_tag="ACHK49_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            complement(3450..4799)
                     /gene="lysC"
                     /locus_tag="ACHK49_004135"
     CDS             complement(3450..4799)
                     /gene="lysC"
                     /locus_tag="ACHK49_004135"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO9264672.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDIA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     regulatory      complement(4891..5087)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            5324..6973
                     /gene="pgi"
                     /locus_tag="ACHK49_004136"
     CDS             5324..6973
                     /gene="pgi"
                     /locus_tag="ACHK49_004136"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO9264673.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALVGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDGKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         1845 a         1722 c         1849 g         1794 t
ORIGIN      
        1 acgataaata cagatacaaa tttagcgaaa agttttccct ctacaaacaa aaataattta
       61 ttaaatcttt atttttcatt tggttaataa ttttttctca attctggcac gcttctcgca
      121 atatctcgta cgcgttgaaa ctgaattaca aagggggtca aaccccgtcg gcacaccagg
      181 aacggccagg ctgttttccg ggcgtgttcc gtcagccttt gacgccaatt atgggttcga
      241 ttcccacatt cattcctgaa atgaatactg gttttaactt attaaataaa aagtgaggga
      301 ttgcgcagtc agcgatcccc gataacgtcg ccgataaaga tgccgggtaa caggcaaaag
      361 ggaattcccc tgtcgcttcg tgtctgcttc cgcgacggct gttaagcaag gaatagataa
      421 tgattaaaaa aataagcgta agaaaagatc agctggcatt actctccaga aacggcgatt
      481 actacaaagt gctacatgca ggcgagcatc ttttaccctg gttaaatacg ccagaagtct
      541 tgctgattac gctcgatggc agcgaagttc ccgacgtgct ggcggattat ttgcgccgct
      601 ttcagcctga ttgggtggag aaatattgcc tggtggcaga cttgtcggag attgaagccg
      661 gggcgttgta tatggacggt attttgcagg agatcctgcc gccatcaacg cgccgtttgt
      721 actggcgtgt agaggatgat ttgacactgg tgcggatgaa tacgcagcaa gtccaggtac
      781 aaacggaggt gatgaatgcg gtattgcaac cacggcgcaa aggggcagtt aaaggccgcg
      841 atgcaatact aacggtgcag gttccggcct ggcatgtagg cgtgctgaaa atcgatggtg
      901 aaacgcaggc gctgttaccg ccggggctga ccgcatactg gaaaattaac catctggttg
      961 acgctgaagt ggttgacacc cgcttgcagg tactggaagt cagcgggcaa gaaatcttaa
     1021 cgaaagataa ggtcaatctg cggattaacc tcgccgctaa ctggcgctac agcgacgtgt
     1081 tattggcatt tagccagtta acgaagccga ttgatcattt gtatcgggaa ttacaatttg
     1141 ccctgcgtga agcggtaggt acgcgtacgt tggatgaatt gctggaagat aaacaggtca
     1201 ttgatgacgt tgtcagcgaa caggtgaaat cgcgcatgtt gccatttggt atggagattg
     1261 cctcgttagg ggtgaaagat atcgtcctgc ctggcgatat gaaaaatatc cttgcgcagc
     1321 tggttgaggc ggagaaatcc gcgcaggcca atgtgattcg ccgacgtgaa gaaacggcag
     1381 cgacgcgttc gttactcaat accgctaaag tcatggaaaa caacccggta gcgttacgct
     1441 taaaagaact ggaaacgctg gagagagtgg ctgaacggat cgataacatc tctgtatttg
     1501 gcggtctgga tcaggttctg catgggttgg ttaatatcaa agggtgagta acatgccatt
     1561 aaatggagtc agtgctgcga tgcgagaaag ggtatcgcag cagttaaaag aaattgaacg
     1621 gcggtatgat gtgaaagtac tgtacgcctg tgaatcaggg agccgcggct ggggatttgc
     1681 ctcgcccgac agcgactacg atgtacggtt tttgtatgtt cacccgctgg aatggtatct
     1741 gcgagtagag tcaccgcgcg atgttattga gctgcccatc gacgacgagt tagatgtttc
     1801 cggttgggag tggcgtaagg cgctgggatt attgaaaggc gcgaacccga cgctgataga
     1861 atggctggat tctccggtgg tttatcaaca agatgaagaa acgataacgg cgtttaaggc
     1921 aatggtccca atgtggtttt ctccgctgcg tgcgcgctgg cattactatt cgatggcgca
     1981 gaaaaacttc cccggatatt tgcagggcga cgaagttcga ctgaaaaaat acttttacgt
     2041 gctgcgtccg ctgctggccg tgcgctgggt cgaagcgggt aaaggcgtac cgccaatgcg
     2101 ctttgctgag ttactggcgg gaagcgaact tgatgcgccg ttacgtgcag aaatagacga
     2161 attactggaa cgtaaacagc gcgcaggaga agcggaatat ggtccgcgcc gtccattgtt
     2221 acatgcgttt attcgtgcgg aactggcgcg tggggaaatt ccaccgctgt taccggatag
     2281 cagagaaggg gacgttaagg aactggatag cttaatgtac cagacggtaa tgcgccgggc
     2341 ataaagcccg gcgttatttc acgccatcag ctcttattat tattgtgtat tcccgctgat
     2401 ttcattctgt ataagcgttg caatatcgtc attcgttgcc gcttgtgaac taaatatcat
     2461 cttctcacgc gaatacaccg caaacttatc tgacaggttc gaagcaaaca ccttgcgggc
     2521 attttctgcc agcaaaccgg tgacgatatc atggtggttt ttatccactt ttcgggcaat
     2581 atttcccgcc tctttgtcct tactataccg ttcattggtg ctttcccagc ttaatgccgc
     2641 tgaggttgtt tttatcatga cttctgtttg gtagcctgca tctttgagtt ttttaatgtt
     2701 tgtaacggcg tcgcggcatt acgaaatgtt ccttcgacaa caatgttgag tttactgact
     2761 atcgctctct caattgcacg ttcgaccgtt tcgccggaaa atttcgctgt atgggtaaca
     2821 aaatcatccc cataattttg gtagatataa ttaaaatcag ggtgtagaaa tcgaaaatca
     2881 tcaccgttaa taatcattat attatctttt gtctctcttt tggctaattc aatcagagat
     2941 gatttacctg ctccgggttg cccacctaaa acaaaaccaa tgggatgctg ttgcggtgtg
     3001 acttcatgat ttgtcagacg ctgccaaata gaatcaaaag atgctttatt ttctgaatgc
     3061 gtagtattgt tgaattcatg ctgatattga gttcttacct gctgaaggag aatcttacct
     3121 tgtgtaaaat caacaagttc agcgggggta gaataaagat aatcgcgata gctgactaat
     3181 cgcttgagag catcgcctga atcctgatgc gacgaaactg agcaattatc aatgatattt
     3241 gataaaaacg cgatgcactg actggctccg tttaaaagtg catctttttc cattaaagta
     3301 gccattaaca ctcctgttaa atgatatttt tcggtgatta taatactgtt ctacaggagt
     3361 gattttgctt ttcagatatt atttaatcta tgttttcagt gaggatggcg tataacaatc
     3421 ggtgagcgtt atacgccagt agggaaatat tactcaaaca aattaaaatg cagcttctgc
     3481 accacctgct cggcatcttc gccgggcacc aggaagcaca ggttatggct ggatgcgccg
     3541 taacaaatca tgcgaatgtt gaacggttcc agtacgccga atacctcttt gccaacgccg
     3601 caggcttttg acaggtcatt gccaatcaac gcgaccagcg ccagaccttc ttctacttcc
     3661 acccggcaca gtgcggaaag ctccatcagc agagattgcg tcagcaacgt atcgccagtg
     3721 gaggttgaac cggtggtatc aagggttaat gccacgctca cttctgacgt ggtgattaag
     3781 tctaccgaaa tattatgccg cgcgaggatg ccgaaaactt ccgcgaggaa gccgcgagaa
     3841 tgcagcatat tcaggctgtg caaagtgagc agagtctgat tgcgacgaag cgccagcgcg
     3901 cggaacagcg gcggattttc agttttattg cacaccagcg taccacctgc gcgtgggtct
     3961 ttgctggagc cgacaaagac cgggatatcg ctgcgtactg cgggtagcaa cgttgccgga
     4021 tgcagtactt ttgcaccaaa agttgccatc tctgccgctt cggcaaacgc gatttcatca
     4081 atgcgttttg ctgcggaaac tacgcgtgga tcggtggtgt agatgcccgg gacgtcggtc
     4141 cagatatcaa cacgagatgc gtgtaaagcc tccgccagca aggctgccgt ataatcgctg
     4201 cctccacggc caagcgtcgt tgtacgacct ttattttcgc taccgataaa tccctgggtg
     4261 atcactaagc cttcattgag acgtgggagc agctgcagcg cggccagttc cgccagcgcg
     4321 gctatatctg gctctgcacg accaaatcgg tcgttggtac gcatcacttt acgtacatca
     4381 aaccactgtg cctgaacatc gcgttcgcgc aggatctcaa caaacagcag ggtcgacatc
     4441 agctcgccgt ggctgaccag ctcatctgtc agcgccggag acgttgccag cgccgccgct
     4501 tctgccagaa cagtaatgtt ctccagcaga cgttcaatct cttcacggat aacgttcggg
     4561 taacgcagac gttccagaat ggcaaactgg atgttgcgga tagcgtcgag tttttcgaat
     4621 cgctcgccag gttccagtcc ttcagctaaa gcgaccagca gattagtgat accagcagaa
     4681 gccgagagga caactaaacg cacgttggca tcagaaagca caatatcagc gctgcggttc
     4741 atggcgtcaa aatcagctac gctggtaccg ccaaatttgg agacaacaat ttcagacata
     4801 actacctcgt gtcaggggat ccattttcag ccttggcaca agggaagagc ggaagacggg
     4861 tgggcgcaga gcgatacttc gctactattt tcacccagaa gtgctccacc acttgcgaaa
     4921 cgcccgactg cgaacgcttc tggtgacaac ccaggggatt cagcccctgt agccgatgat
     4981 gaacgtggcc agccgttcaa tcacctcggc gatgcacccc ctcaggtgtt atcacaggac
     5041 tggctcctcc aacaccgtta cttgggcaac gcgcctcttc tggcctgcgc tagcgcaggt
     5101 agtacattta taaataaagg gtgagcgggg cggttgtcaa cgacggggtc atgcggattt
     5161 ttcatccact cctggcggtc agtagttcag ctaataaatg cttcactgcg ctaagggttt
     5221 acactcaaca ttacgctaac ggcactaaaa ccatcacatt tttctgtgac tggcgctaca
     5281 atcttccaaa gtcacaattc tcaaaatcag aagagtattg ctaatgaaaa acatcaatcc
     5341 aacgcagacc gctgcctggc aggcactaca gaaacacttc gatgaaatga aagacgttac
     5401 gatcgccgat ctttttgcta aagacggcga tcgtttttct aagttctccg caaccttcgg
     5461 cgatcagatg ctggtggatt actccaaaaa ccgcatcact gaagagacgc tggcgaaatt
     5521 acaagatctg gcgaaagagt gcgatctggc gggcgcgatt aagtcgatgt tctctggcga
     5581 gaagatcaac cgcactgaaa accgcgctgt gctgcacgta gcgctgcgta accgtagcaa
     5641 taccccgatt ttggttgatg gcaaagacgt aatgccggaa gtcaacgcgg tgctggagaa
     5701 gatgaaaacc ttctcggaag cgattatctc cggtgagtgg aagggttata ccggcaaagc
     5761 aatcactgac gtagtgaaca tcgggatcgg cggttctgac ctcggcccgt acatggtgac
     5821 cgaagcactg cgtccgtaca aaaatcacct gaacatgcac tttgtttcta acgtcgatgg
     5881 gactcacatc gctgaagtgc tgaaaaaagt aaacccggaa accacgctgt tcctggtagc
     5941 atctaaaacc ttcaccactc aggaaaccat gaccaacgcc catagcgcgc gtgactggtt
     6001 cctgaaagcg gcaggtgatg aaaaacacgt tgcgaaacac tttgcggcgc tctcaaccaa
     6061 tgccaaagcc gttggcgagt ttggtattga tactgccaac atgttcgagt tctgggactg
     6121 ggtcggcggc cgttactctt tgtggtcagc gattggcctg tcgattgttc tctccatcgg
     6181 ctttgataac ttcgttgaac tgctttctgg cgcacacgcg atggacaagc atttctccac
     6241 cacgcctgcc gagaaaaacc tgcctgtact gctggcgctg gttggcatct ggtacaacaa
     6301 tttctttggt gcggaaactg aggcgattct gccgtatgac cagtatatgc accgtttcgc
     6361 ggcgtacttc cagcagggca atatggagtc caacggtaag tatgttgacc gtaacggtaa
     6421 cgttgtggat taccagactg gcccgattat ctggggtgaa ccaggcacta acggtcagca
     6481 cgcgttctac cagctgatcc accagggaac caaaatggta ccgtgcgatt tcatcgctcc
     6541 ggctatcacc cataacccgc tctctgatca tcaccagaaa ctgctgtcta acttcttcgc
     6601 ccagaccgaa gcgctggcgt ttggtaaatc ccgcgaagtg gttgagcagg aatatcgcga
     6661 tcagggtaaa gatccggcaa cgcttgacta cgtggtgccg ttcaaagtgt tcgaaggtaa
     6721 ccgcccgacc aactccatcc tgctgcgtga aatcactccg ttcagcctgg gtgcgttgat
     6781 tgcgctgtat gagcacaaaa tctttactca gggcgtgatt ctgaacatct tcaccttcga
     6841 ccagtggggc gtggagctgg gtaaacagct ggcgaaccgt attctgccag agctgaaaga
     6901 tggcaaagaa atcagcagcc acgatagttc gaccaatggt ctgattaacc gctataaagc
     6961 gtggcgcggt taatcatcgt cgatatgtag gccggataag gcgttcacgc cgcatccggc
     7021 aaccgatgcc tgatgcgacg cggtcgcgtc ttatcaggcc tacaggtcga tgccgatatg
     7081 tacatcgtat tcggcaatta atacatagca cgattgatta aataacctta ataacaatgc
     7141 cgacgttatg tcggcatttt tttatcagat aaatcccctt gtctgtgatt taacggaaat
     7201 cataccatga
//
LOCUS       DAZGLJ010000226         7139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000226 DAZGLJ010000000
VERSION     DAZGLJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.226"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..296)
                     /locus_tag="ACHK49_004137"
     CDS             complement(<1..296)
                     /locus_tag="ACHK49_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9264674.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRV"
     gene            complement(293..2905)
                     /locus_tag="ACHK49_004138"
     CDS             complement(293..2905)
                     /locus_tag="ACHK49_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9264675.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVHRHFSSLEADARKTASTV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFLIMLQQGGQISD
                     SFGGPLSLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGELGRFMSVARVAA
                     GGGVAVLAALAAAAWQAEQADRALLRSLILTGGAAATTTAELWKMAGVISDEAGGGIR
                     QAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEGIAADPVKALAS
                     LNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQLDKAATPVEKIW
                     DDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLALAEFIQSAWETT
                     KNVPGVGALFGELAEENRVFIEKTKRDELALRKSIAERDARIRQGEMGYINRSRATGV
                     SKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEAELRTLEKHSGA
                     NEKISRQRRDLWKAESQYAVLKEAATKRQLSWQEKSLLAHEKETLEYKRQLADLGDKV
                     EHQKRLNELAQQAVRFEEQQSAKQAAISAKARGLTDRQAQRESEAQRLRDVYGDNPQA
                     LARVTGALKQTWADEDMLRGDWLAGLKSGWGEWAESATDSFSQVKSAATQTFDGIAQN
                     MAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGGASASSGTA
                     IEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEG
                     GYVGGAGSPAQMRRAEGINFNQNNHVVIQNDGINGQAGPQLMKAVYDMARKGAQDELR
                     LQLRDGGMLSGSGR"
     gene            complement(2886..3299)
                     /locus_tag="ACHK49_004139"
     CDS             complement(2886..3299)
                     /locus_tag="ACHK49_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO9264676.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGDGGRD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
     gene            complement(3326..3748)
                     /locus_tag="ACHK49_004140"
     CDS             complement(3326..3748)
                     /locus_tag="ACHK49_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO9264677.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYIKFVSDAEQQETTKHD
                     VVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEQTGKAEATEPVTSKKHSKAS"
     gene            complement(3762..4514)
                     /locus_tag="ACHK49_004141"
     CDS             complement(3762..4514)
                     /locus_tag="ACHK49_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9264678.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VTAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(4522..4917)
                     /locus_tag="ACHK49_004142"
     CDS             complement(4522..4917)
                     /locus_tag="ACHK49_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO9264679.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(4914..5492)
                     /locus_tag="ACHK49_004143"
     CDS             complement(4914..5492)
                     /locus_tag="ACHK49_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9264680.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGASAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(5504..5857)
                     /locus_tag="ACHK49_004144"
     CDS             complement(5504..5857)
                     /locus_tag="ACHK49_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO9264681.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(5869..6264)
                     /locus_tag="ACHK49_004145"
     CDS             complement(5869..6264)
                     /locus_tag="ACHK49_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO9264682.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(6306..>7139)
                     /locus_tag="ACHK49_004146"
     CDS             complement(6306..>7139)
                     /locus_tag="ACHK49_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO9264683.1"
                     /translation="SRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLADPAYRR
                     RRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAANNITQSG
                     GTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVRDKLDTRRGS
                     HSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGNTQARGL
                     RTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDEFVSVQL
                     A"
BASE COUNT         1438 a         2326 c         1705 g         1670 t
ORIGIN      
        1 tcaacgcgca acatcccgac ccgcgcagac cacccggcac aggtcacctt tatctgccgg
       61 tatgcatagg gtggcttcca caaaaatgcc ttccagcctc cgtgctctgc caggaacgct
      121 tccagatgcc gggcctcctc ccgggtcacg gaaagcgtca cacggtatgt tttcaggtca
      181 gcattcagcc ctgccgccat acgctgtgag tacccgtcac caaaacgcac ttcacgcacc
      241 gatggctgcg agttcacctc catatccggc ttcactttcc agcgaaatgt tttcatcgcc
      301 cgctccctga taacataccg ccatcacgca actgcagccg gagctcatcc tgcgccccct
      361 tgcgggccat gtcatacacc gccttcatca gctgcggccc cgcctgtccg ttgataccgt
      421 cgttctgaat caccacgtga ttgttctgat taaaattaat accttccgcc cgccgcatct
      481 gcgccggact tccggcacca cccacataac caccttccgc atagccgcgc atcagacggt
      541 aaagattccc cacacctatc cggctggttg cctctttcgt gaaaacaaac tccccgcggt
      601 gaactatccc cgcaggctca tatttgccgc ccgtccccgt aaatcctccg gtcgcgaaat
      661 ggaagttcgc cgccgcagcc tcaatggccg tccccgagga agcagatgca ccaccaccga
      721 aagcaccgcc aatggcgctg ccgatacgcc cgacaatgcc caccatggcc tgtttaagca
      781 ggatttctgt catcatggac agcaccgaac gggtgaatcc ccgccagtct gcctctgcac
      841 cggtcagcat cgccgccata ttctgtgcaa taccgtcaaa ggtctgcgtg gcagcacttt
      901 taacctgcga aaaactgtcc gtcgcacttt ccgcccactc cccccagccg gacttcagcc
      961 cggccagcca gtcaccgcgc agcatgtctt catccgccca tgtctgtttc agtgccccag
     1021 tgacccgggc cagcgcctgc ggattatcac cgtacacgtc ccgaagacgc tgcgcttcag
     1081 actcccgctg cgcctgacgg tcagtgagac cgcgggcttt tgcgctgatg gcggcctgct
     1141 tcgcgctctg ctgctcttca aaccgcaccg cctgctgtgc cagctcattc aggcgtttct
     1201 ggtgttcaac cttgtcgccc aggtcagcca gctggcgttt gtactccagc gtctctttct
     1261 catgggccag cagggatttt tcctgccagg ataactgccg tttcgtggca gcctctttca
     1321 ggaccgcata ctgactttcc gccttccata aatcacggcg ctgccgactg attttctcat
     1381 tcgcaccgct gtgtttttcc agcgtcctga gctcagcttc aagggcaagc agagcctctc
     1441 ttgcctgctc ctcttccctc tccccggcag agcgcgtttt cggtgatgta tgctttttac
     1501 ctgtcagctc ttcagccaga cggctgacgg cttcctgctg cccaggacct ttgctgacgc
     1561 ctgttgcacg cgagcggttg atgtacccca tttccccctg gcgtatacgc gcatcccgtt
     1621 ccgcaatgga ttttctcagc gccagttcat cgcgttttgt tttctcaata aatacgcggt
     1681 tctcttctgc cagttcacca aacaacgcac caacgccggg cacattcttt gtcgtttccc
     1741 aggctgactg aataaattca gccagcgcca gatccccctg cacaagcagc agcttcactt
     1801 gttcaacggt tccggccacc acgtcagtga tcagactgag tgcccccagt gtatgatcac
     1861 ctatccatgc ccatgcgtca gaagtccagg ttttaacatc gtcccagatt ttttccaccg
     1921 gcgtggccgc tttatcaagt tgctccagac gtgcattcat gacatccgca aacagggaca
     1981 tcgcctccgt caccgcagcc tgtttacctt tcgtgcgctc aagctcatca atatggcgta
     2041 actgggaaac gctcaggaag ttatactgct gattcaggga ggccagcgcc ttcaccggat
     2101 ctgctgcaat cccttcaaag gctttttcca ccttcccggc atcgtccccc accgtctgca
     2161 gccatctctg agaggtttcc cccatgatcc gtagctgccc ggcggtatat ttcccgcttt
     2221 ctgccagacg ggccagattt tctgccgcct gtctgatacc accaccggct tcatcgctga
     2281 tcaccccggc cattttccac aattctgccg ttgtggtggc agccgcccct ccggtcagga
     2341 tcagtgaacg caataaggcc cggtcagcct gctctgcctg ccaggcggcg gcagcaagcg
     2401 cggccagtac ggcaaccccg ccacctgccg ccacacgggc caccgacata aatcgtccca
     2461 gctcaccggc attccgggca ttttcagcca gtgcatttgc cgtatctgac agcgactcct
     2521 ctgatgattc agaggcatcc ctgatcccga gaagttcctc cttcagcagg gtaagcaggc
     2581 tgagcggtcc accgaatgaa tcgctgatct gccccccctg ctgcagcatg ataaggaagg
     2641 gattctgacc accggcaagc tgagtgacaa tatccgtgaa ctgtgcgggc agtgtgcgca
     2701 tggcagcctt atactgtccg actgatatcc cggctttttg tgcagccagc gcctgtcggc
     2761 tcagcccctg ttcaacagta ctggcggttt ttctggcatc cgcctccaga ctggaaaaat
     2821 gacggtgtac ccgggccatc tgttcatcaa accgggccgc atccagactc aaatcaataa
     2881 ccagatcacc cgctggctgg gacatatctc acacctccgg aaatccccgc tgaagccatc
     2941 attaatgcaa catcatcctc gctgacatcc accacatccg cagaaggtga aatatcgcgc
     3001 cctccgtccc ctccgaaccg gacgcctccg gcaactcctg tcgctttctg catcagcatt
     3061 tcttcctcgt ccggcatctc cgtctgctct tcctcacacg ctggagcaag cagactgaaa
     3121 tccgccggat gcatatccgg atcgccaaaa aacaggctga gtacggcgta catcagcccg
     3181 gaaaaatgag cgtccagttg ggtatcctga aaataatgcg tgcagtaaaa acgtcgccag
     3241 tcggcatatt cggtggatgt catcccggca agcatggcgc gccagtcggg tctccccatc
     3301 tctcgcgcca gtttcaggac aaagttcagc tcgccttcga atgctttttt gatgttaccg
     3361 gctcagtcgc ttctgctttc ccggtttgtt caggatcggc atcgtgccgg ttatccagca
     3421 tacctgaaag ataaagcacc cggttcgttg cctgattcag tgcatcagca ggccatccca
     3481 gcatcacttc acggcggatc tgctgcatct ctgtctccgg agaggccaga gtgcctttga
     3541 gggaatggga atgccatagc gacatcgcca caagcaggga tgccgtttcc agatatcgct
     3601 ggttaatgtg cacgacatca tgcttcgttg tctcctgttg ttctgcgtct gaaacaaact
     3661 ttatataatc aaaccgctgc agcgcagaca attcggaaag cgtgacggac acaccgttat
     3721 attcaaattg ttctgttttc agaaacatgt attacctccg tttaccctgc agcgcccgct
     3781 tcagtaacgg tgacttcagc cactgcggcg aactgaccat ttccgctcac cacagggatc
     3841 tgcaccttac ctgtcgccac gccgtttacc gtaattgtca tatctttcac actaatggtg
     3901 gctttcgacg gatcggcgga aaccgctctg aacgtcttgt cggttgcact ttccggctca
     3961 aaagaaaccg tcagggtggt tgttttccct tttgccaccg taccggatgt cggtgtcacc
     4021 ttaatcgcag tgaccggcgt aattttgctg cgttcttccg ctacagaagg tttgcccacg
     4081 ttggtcactt tcaccgtgcg ggtgatcact tctttcgccg tcacggcctt accgatactg
     4141 ctgacccagc cacggaacac atccaccgtg ccatttggga aacggatttt ataggcccgc
     4201 acatccccgc tttcaaacca gcctataagc cctttctgac cttcttctcc cggtttccag
     4261 gccagcgtaa aactggtatc tcctgcagac ttctgcccct gcccggtcgc ggtccagtcc
     4321 gcgtcttcat catccaggta gttatcatcg tagggttctg ccgtcatctc gcccggcgtc
     4381 agatccttca ccttagccag tcgctgccag tcatcgtctg acaacgggtt tgcataagca
     4441 tcacccttgc cgttgtaaac ccacagagtg gtaccggcac cttttaccgg ctcaagggga
     4501 tttggtgttg ccatatcgtc ctcacatctc gtatgtaatg gaataagtca gatctgcaga
     4561 actccataac gccatatcgt catcacgacg atactcatag ccctgcgtaa ccatcgtggt
     4621 aatcagtcct gccagtgccg ggatatcgct catcaccgga taaatccggg actccatcca
     4681 cgcatccagc tctgaatccg gcacctgagc aggcaggaaa acttcaatat gcagctccgc
     4741 ctgccaggta tcgctgtcca gctcttcgcc cgtgtattca gcgccggtga gataaacggc
     4801 aactgccgga aaatccgcct catcaaaaac agcggggcga ccatcaaaaa gcgtcgcccc
     4861 ggtgtcatgc ttctccagtg catccagtac ggctgcacgg agttcagtat gtttcatcgc
     4921 tttattacca ttctcagttg atgctgcagc gcatagccca gctctttcgg aagacgttca
     4981 cgccgtatcc gttcaatatt ctgtttaaac gccgtggtaa gcggcaccgc catcgggatt
     5041 ttcaccacat caatggggta acggtttttc ccggccacac gctgcatgac atgccaccgc
     5101 ccatttttca gttgctgaat aaacgcgccg ggaatacgac ggtttcccac cacaagcacg
     5161 ctgccgccac ctttcaggga tgaacgctgc ccctttttac gacgcctgcg tcgggacagg
     5221 acaacccgcg cattccccag cttgattacg ggcaaatccc cccggttaac cttgattctg
     5281 gcctgcggat ttttaaccgt ggcccttttc agcctggccc tttcctttac cagtttccgg
     5341 cgtacctttg tctcacgggc aacctgtgac gcagactgcg atatcgcgga tgaagcaacg
     5401 cggttaatgg ccattgctga cgcaccgggc accgccgttt tgctgatacg gctgaggttt
     5461 tcaacggcct gctcaagacc ttttatggcc atacatcccc ctttcagcgg cgacggttaa
     5521 cggcaggcgg cacgccacgc ccaagccaga gatgacagct tccgccatca tccggcgaaa
     5581 tccggtctat ccagaagttt tcctcaccga tggtcagcgt gtctccacgc cgcagctgcc
     5641 gcacatcatc agtccggaca aacagggacg ggctggagcc ttcaacgcgc acgccctgtc
     5701 cggcatagct gatattttca gggtcatcaa aaacaccacg tattactgcg ccggactgct
     5761 caccggatgt catggtggct gacgttccca tgtacccgcg tatcgtttca tcggcgcggg
     5821 caatggcagc atcgaacagg ttatcgaaat cagccacagc gcctcccgtt attgcattct
     5881 ggccaggcca cgttctgtca tttcggctgc cacaccggca gagacacgaa acgccgttcc
     5941 cggcagcaca aatgccacag gttcatcccg cgtggcgtga agtgcatcgg tatgcagcgt
     6001 caccagtgcc acaaccgtga ccagagcagc cgtatcagtc acggtatccg tctgtgctga
     6061 taccacctca ttttcatgtc cggtcagcgc attttccggg ctgacagacg tgtcctgacc
     6121 ggctgcgtca tccgtgtcat caagctcctc ttccagctct gccacacgga gcgccagttc
     6181 ttctttcgtc cccgtcaggc tgacatcacg gttcagttgt tcacccagcg agcggagacg
     6241 ggcaatcagt tcatctttcg tcatggactc atccacagag aaacaatggc cccgaagggc
     6301 catgattacg ccagttgtac agacacgaac tcatcagggt cagccagcag catcagcggt
     6361 gctgactgaa tcatggtgaa ctctcgcgcc ggatcgccgg tagtcaccca gtttttcggg
     6421 taacgggcag aggcgttaat gccttcgcgc tgtgcgtccg catcctgaat acagccatag
     6481 gtacgcagac cgcgtgcctg agtgttcccc agcaccatcg tgttgtccgg caggaagttc
     6541 tttttgacgc cgttttccac gtactgtccg gaatacacga cgatggccac atcgccatac
     6601 attcccttat aagacaccgc tttgcccagg tcttttaccg ctgtctccag ctcggaatga
     6661 gagccgcgac gggtatccag cttatccctg acggctttga aggaacggaa cagcgcccat
     6721 cctttcggat caaacacgat gatattcacc acgccgctgg cgttcagcgc gtaggcttcg
     6781 atatcgtcgg tcgggtcata cgtggacttg tcacgcttgc tccactccgt gccaccggac
     6841 tgtgtgatgt tgtttgccgc actgcggccc atatccacct caaccggatc aaaggcttca
     6901 ccggtcatgg tgtatttgcc cttaagcacg gcagaaactg cctgcatctc ttcgacctgg
     6961 gcaatggcca gctcttcgtc acgcatgttc tgcatgatga tgcgacggcg gcggtaagcc
     7021 gggtccgcca gattctgcgg atcttcatcc ggcaggcgac gcagggtcat ctgcggattc
     7081 acctcatgct tcggcttgac atatcccggc gtaaattcag aggtggagcc gccacggga
//
LOCUS       DAZGLJ010000227         7122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000227 DAZGLJ010000000
VERSION     DAZGLJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.227"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(236..844)
                     /locus_tag="ACHK49_004147"
     CDS             complement(236..844)
                     /locus_tag="ACHK49_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001415151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFO9264684.1"
                     /translation="MVTQLAFRDVKFTPINHNNQIWFTSKELAAALKYASTKAVTDIY
                     NKNIDEFTDGMSQVVESTTSGNYRKKTRIFSLRGAHLIAMFARTPVAKEFRRWVLDIL
                     DREIQQSPITKQFTDNELCTLAWLWRASDTMLTACQNVTPLLQVAEHREAGRFTSIEQ
                     EYPRILNKAQAILARETAHVKFRPWQDDKWSRVLTHLRSERL"
     gene            complement(1270..1338)
                     /locus_tag="ACHK49_004148"
                     /pseudo
     CDS             complement(1270..1338)
                     /locus_tag="ACHK49_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1597..1944
                     /locus_tag="ACHK49_004149"
     CDS             1597..1944
                     /locus_tag="ACHK49_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264685.1"
                     /translation="MTIKATDIEILHRYAEGVMERSNHHAKNVGAAALTLLGGVIWKA
                     LPGSIEIRTYNGSLANMVWWQSERTLKNYAISYNHNSCEIEMRDESVKGAVLFSISNE
                     TTPEKILSQLSEL"
     gene            complement(2149..2850)
                     /locus_tag="ACHK49_004150"
     CDS             complement(2149..2850)
                     /locus_tag="ACHK49_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO9264686.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVSAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(2847..3866)
                     /locus_tag="ACHK49_004151"
     CDS             complement(2847..3866)
                     /locus_tag="ACHK49_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO9264687.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGTYPNQVNTKDKLTKDNIKPYSSENSGESSDLPENDLPVVK
                     ADAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTVGKPKLDLTNTDWI
                     YGVDL"
     gene            complement(3863..4402)
                     /locus_tag="ACHK49_004152"
     CDS             complement(3863..4402)
                     /locus_tag="ACHK49_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO9264688.1"
                     /translation="MQPLPYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRTRQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPELAGPLMAMVTTMLGAI"
     gene            complement(4472..4702)
                     /locus_tag="ACHK49_004153"
     CDS             complement(4472..4702)
                     /locus_tag="ACHK49_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9264689.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            4807..5496
                     /locus_tag="ACHK49_004154"
     CDS             4807..5496
                     /locus_tag="ACHK49_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO9264690.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            5578..5841
                     /locus_tag="ACHK49_004155"
     CDS             5578..5841
                     /locus_tag="ACHK49_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264691.1"
                     /translation="MSNKTLVKKSNNCVDAYADAFGYSSFGDGSQRLGSISFFHNVTE
                     WPVDESDDVENIKYNIATIRMPEELMLKLADFIRDQHDKAKTN"
     gene            5854..6297
                     /locus_tag="ACHK49_004156"
     CDS             5854..6297
                     /locus_tag="ACHK49_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264692.1"
                     /translation="MKHDYYEAPNLNDKIAKAAESFCLHSSASSQLAGISGKRWDMLQ
                     SSGSSGSGSDNMPEKVAKLESDVAHIKRDVDELKTDVKAIDKNMITILARLDSIKESL
                     TNKPSSDAVDRKISDAKLAVLLGVPAIIAAGTGLYKLSMYFFFSA"
     gene            6848..7054
                     /locus_tag="ACHK49_004157"
     CDS             6848..7054
                     /locus_tag="ACHK49_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO9264693.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKRKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNALNH"
BASE COUNT         1887 a         1681 c         1584 g         1970 t
ORIGIN      
        1 tgaaaacaat attggatatc attttcggta tttctctgca aattccagcc cacaggatct
       61 cggtgtacaa tctgtcgctg atcgcgcaaa tttagggtca atcacttcat tttttgcatc
      121 aaaccctcac taacatcgaa cgattatagt acaagtagtg ttcttgactt ctcattaaac
      181 cgtcagcatt caattaatta caaaatacta gcggcatttc tctcccgcaa ctttattaca
      241 gccgttcaga acgtaaatgc gtcaatactc gactccactt atcatcctgc cacggccgga
      301 attttacatg tgccgtttct ctggcaagga ttgcctgtgc cttattgagt atccggggat
      361 attcttgttc gattgaagtg aagcgaccgg cttcgcggtg ttctgctacc tgaagaagcg
      421 gagtgacgtt ttggcaagcg gttaacattg tgtcacttgc ccgccataac caagcaagtg
      481 tgcaaagttc gttatcagtg aattgttttg tgattgggga ttgttgaatt tctcgatcga
      541 gaatatccag cacccagcgg cggaattctt tggccacagg agtgcgggcg aacatggcga
      601 tcagatgggc gccacggagg gagaaaatgc gagtcttctt acgataattt cctgaggtcg
      661 ttgattcgac gacctgagac attccgtcag taaactcatc aatatttttg ttgtagatgt
      721 cggttactgc tttggtgctg gcatatttta atgctgccgc caattctttg ctggtaaacc
      781 agatctggtt gttgtgattt ataggagtga attttacatc gcggaatgcg agctgagtga
      841 ccatagcgat aatctcgttt tgaatttgag ctatcaccac cgacgacgcc aatcgactgg
      901 tggtggactg tgcaaggttg gcgtaaccgg tcaaaacgaa atccggcgct tccgaagaag
      961 ccctcacaca gcccaccata atttttgtgt agacgtgcac tgcgcataaa aaaaccgcga
     1021 acgcggttat gcgtcgtttt gatgtccggg acgccaatcc cgtgtgccga ttttgcggca
     1081 acgcacagaa tatagcgccg gataaataat gtcgtcaacc cttgcatatg gggggcgcga
     1141 tgatcactac taccaccata atcgctccaa ctgttgcaac tactttaggc taagacatca
     1201 cgatttccat atcaccttta gccgccagac aaacagagac tccctgttct tggctgccaa
     1261 aaacaccgaa tgcaaaaaat cgcaggtgta tttctgcatc tgtggaggga gaagagagtt
     1321 tggattgtat gtgcgcataa acgtccccgt ttagcgcaga agtcaccgta gttgttcata
     1381 ttctgatgac atgataattt cctattgaat agaaaaaatt aacacgactc attcttatga
     1441 ctggaaaaag caatcatgct aataatcatt gttgttccag ttccaaatgc agcgaaataa
     1501 tcaccaatat tttttctacc ggtttcgact ttgatgctat attctgctaa cacctaacta
     1561 acactacgtt tcacacagca attacatgga aataacatga ctatcaaagc gactgacata
     1621 gagattcttc atcgttatgc tgaaggggta atggaacgtt ctaaccatca tgcaaaaaat
     1681 gttggtgcag ccgctcttac attgctagga ggtgttatct ggaaagccct gcccggatca
     1741 atcgagataa gaacgtataa cggaagtctt gcaaatatgg tgtggtggca aagcgaaaga
     1801 acattaaaaa attatgcaat ctcatacaac cataattcat gtgaaattga aatgagagat
     1861 gaatcagtca aaggtgctgt attatttagc atttccaacg aaaccactcc agaaaaaata
     1921 ttatcacagc tttctgagct ttaattaaag gcggtgctaa caccgcccat accattactc
     1981 cacatcttac gcaacttgat aattatgctc tattaaatac ccacaatatt tcatcaatgc
     2041 atgggtcagc tacaaaatgt ttaccgtggt aattcctgtt ttcgcaacat ctcaaaagaa
     2101 caataaacac tgtatttctt caagtaacga agaatctttc tttgcccgtc atacacttgc
     2161 tcctttcagc ccaaacttag ctttgatttc tgcgatcttc gccagagcct gtgcacgatt
     2221 tagaggtcta ccgcccataa caggaagttg ttttactggt tcaggtatcg tctcaccacg
     2281 gttaattcgc gctgtcatac aggtcagttc atcggcagcc ttgcgccgta attccgcgtc
     2341 agtcagcgca ttggcccgca tgttctggta caggttggta accaaccagt aatgcgcgtt
     2401 cgatttccac ggataagact ctgcatccgg atacaggcca cgcttccggc aatactcgta
     2461 aaccatatca accagctcgc tgacgtttgg cagcccggcg ttaacggatg cttcttcccg
     2521 gcaccatgca acaaactgcc cgggtgatgg cagaaatggt ctattctgcc gacgggcaac
     2581 acgcattccg gcgctcacct gttccatcgt ggtgatcccg ttttcccgga aagccagaac
     2641 ccactggcgg cggatttcgt tcagttcgtt ctggtcacga ttagccaggc tcgccgggaa
     2701 agttgccagc aactggctga atacaccgtt gattatctgc gctacctgct gtacctgcgg
     2761 cttttcgtcg tactgttccg gcatgttgtt ggcgatccga cgcatctgct cacggtcaaa
     2821 gttaatcatc tgtgcggcga tgtttttcat agatccaccc cgtaaatcca gtctgtgttt
     2881 gtcaggtcga gttttggttt gccgactgtc acgccagcct gttgcttgtt ccggttgatt
     2941 tcgagctggg tccacttgtc gcggagtttg gccggactca gcacgttacc ggaccagaag
     3001 ttgtcctggc aggcccagcg gaacagtaca cacatgtcgc ggtggttacg tccatcacgt
     3061 tcacgcatca gacggatatc gttagcccac cctgcaaaat tcggttttct ggctgatggc
     3121 gcgatggtct tcaccatgtc aaacatccac tctgcggcgg tcaggtcttc tgctgtcccc
     3181 cacttgctgc cgctctgaat cgcagcatcc gctttcacca caggaaggtc gttttctggc
     3241 aggtcagagg attcgccaga attctcggac gaataaggtt ttatattgtc ttttgttagt
     3301 ttgtcttttg tgtttacctg attcgggtaa gtgcctttac ctgatttggg taaacttttc
     3361 ttacctgatt caggtaaatt tacctctttc aggtaaactt tatttttctt acctgattcg
     3421 ggtaatgttg accattcact gaccacatta ttaatgccga tattccgccc gctctgaata
     3481 aaaatcccac gctttaccag aacacttttt gcagcagaac acttgtgcgg caatatcccg
     3541 gtcaactcgg aaagttgctc gttgctcacc cagtccagtt ttttattaaa gccatatgtt
     3601 ttgcgcatga cagccaggaa gaccagaagc tggtgctgtg ttaatccggc cagcattaca
     3661 gcttccagca actcatttgc aatgcgcgta taaccatcat cgagatctgc cacgcgcggc
     3721 tccttttgtg ccgcatccgg cactggaaaa ttgaatatct cagcagtgtt tgccataatt
     3781 cctcccgcaa tgagtgtgtt acgatttgca cctgaaagtc ggttctgttc cagcagaccg
     3841 gctttcgcca tttctgaacc tgtcatatcg cccccagcat ggtagtaacc atcgccatca
     3901 atggaccagc cagctctggg tccacacgaa acatcgacac aataccttca ctaatttcct
     3961 tcagtttctg gtggcgtggt gcgttgagaa tgacagcctg ttttgcctca ctgagttcct
     4021 tttccatttc agccaaccta gccatgaagc tatcctgctc aaccaggtaa ccgcgatatt
     4081 ccagcggtag taccgccaga attgccgggg tcagttcacg cacgttattt cggtattttt
     4141 cagaatcgaa tttgttatcg aggaagcgga acagcttctg gcgtgtacgg ctgacatcat
     4201 cagggaaatc gatggtgccg ccgccctgct cccgatactc attcacaatg agtgtggcaa
     4261 cgacatcctg attatctaca gccgaccagg cgcggacggc atcacggatt ttttcgtggc
     4321 ctggcacctg ttttgtttga gaacgattta tcaccgcagt cgggctaaat ccgctagtct
     4381 gttggtatgg aagtggttgc ataattgact cctttagttt gaattgactg ttaagttgat
     4441 tgcttattgt taaagagcgt gaaatggaaa tttaagctgc gttcttttcg gtgtgtggaa
     4501 acaacttcgg aagatccggg cgaatctggt atgccttcac tactccacca gtagccgtaa
     4561 caatgctgcc gacatgttca ggggatacct ttgctttgtt gtgaagccac ttatagacgg
     4621 cctgctgtga aacttcgcag gcagcgccca gtttcttttg tgaaccaacg atattgatcg
     4681 ctgttttgat agctgggttc ataacaacct ccgtggttaa tttgaatcaa gattaaaact
     4741 atggttgttt ttagtcaaca accattttcg tttgatggaa taaaaccttg gttgtacatt
     4801 tggactatga aaacaacact ctcagaaaga cttaaagaag ccagattagc gcgaggcctt
     4861 acacaaaagg cgcttgggga tttggtcggg gttagccagg ctgctattca gaaaatcgaa
     4921 acagggaaag ctaatcaaac aactaaaatc gtggagatcg cgaacgcttt gggtgtgcgc
     4981 gcagaatggt tatcttctgg cgttggaaat atgtcagaca gtacagtgca accaatacaa
     5041 tcaactgtca gccattccaa atacttcaag attgacgttc ttgatataga agtcagtgct
     5101 gggccgggag tcatcaaccg tgagtttgta gaagttctac gctcggttga gtactcgttt
     5161 gacgatgctc gtcacatgtt cgatggtagg aaggcagaaa atatccgcat cattaacgtg
     5221 cgtggtgaca gcatgtcagg aacgatcgaa ccaggtgatc tgctgttcgt tgatatcacg
     5281 gttaaatctt tcgacggtga tggtatctat gcatttctgt acgacgacac cgcccatgta
     5341 aagcgcctgc aaatgatgaa ggataaactg ctggttatct ctgataacaa gagctactca
     5401 ccgtgggacc ctatcgagaa agacgagatg aaccgggtat tcatattcgg taaggttatt
     5461 gggagcatgc cgcagacgta cagaaaacac ggataatcag ccgcgtgttg atgaggcttt
     5521 tgggtaatac gctgaagaaa ataccttata aaaaataaca ttacgggaaa ggcaaaaatg
     5581 agtaataaaa cacttgttaa aaaatcaaat aactgtgtcg atgcatacgc cgacgcgttt
     5641 ggctactctt cgtttggtga cggctctcag cgcctaggtt caatttcttt ttttcataat
     5701 gtaactgaat ggcctgttga tgaaagtgac gatgttgaga acataaagta caacattgca
     5761 accattagaa tgcccgaaga attgatgctt aaattggctg acttcattcg cgaccagcat
     5821 gacaaagcta aaacaaacta atcgtaatta attatgaaac acgattatta cgaagctcca
     5881 aacctaaatg ataaaattgc aaaggcggca gaatcatttt gtttgcactc cagtgcgtct
     5941 tcccagctag caggtatatc tggaaaaaga tgggacatgc tacaatcaag tggtagcagt
     6001 gggtctggga gtgataacat gcctgaaaaa gttgcaaaac tggagtccga tgttgctcat
     6061 atcaaacgag acgttgatga acttaaaaca gacgttaaag ctatcgacaa aaatatgata
     6121 actattcttg cacgtctgga ctctataaaa gaatccttaa caaacaagcc atcaagtgat
     6181 gctgtcgata gaaagatttc agacgcaaag cttgcggtat tgcttggtgt tccagcaatc
     6241 atcgctgcag gaacaggact ttataagcta tcaatgtact ttttctttag tgcttaagaa
     6301 aatgctcgca cccttgcatt aggtaaagcg atttttcatt ccccattacc ctcgctctta
     6361 accaatcatc taaacccggc caccgtgccg ggttttcttt tgccctcccc tcatcataca
     6421 ccgttcaaaa aaaccaccac gacctcgctt cagttatcgc tatgcgatgc aagtcacaaa
     6481 ataaatccat cttaaataca accagttata tctaaaacaa ccaacaaaac aacttttgtt
     6541 gttgacgata aaacaactat agttttaaat gagttcatcg caacaacaca acgatacggc
     6601 aatcacctga ttcaccgttg cgatgaccgc ttagatccgc agcttgaatt tcagcaggct
     6661 tcggggagtg cgagggatga aacggacgcg tgaacgtcgg tgtgaccagc tgaaatcaac
     6721 tcaacatttc ataccttagt cgcttcaacg aggcggctta gttatgacaa ccggcggcca
     6781 tccaccgcct gaatacgcgc agaagtcttt atatgttcag cagcccagct tacgggcagg
     6841 agtttttatg gttcatcaac attacggaac gcagaccgtt aatcgcggtg cggtcatgcc
     6901 aggaatgctg gtcaaacgca aagatggtac ctggactgca tcagctaatt tacgcggacg
     6961 actttatctg catcgcggca ttgagcgcac ttatacccgt gacttgctcg tggaagtttt
     7021 tctcgacgga cgcggcaacg ctctgaatca ctaatcccct ttcctgtttt ccgaatcagc
     7081 ctggcattcc gcgggcgatt ttttcacagc cattttcagg ag
//
LOCUS       DAZGLJ010000228         7114 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000228 DAZGLJ010000000
VERSION     DAZGLJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.228"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(14..1123)
                     /locus_tag="ACHK49_004158"
     CDS             complement(14..1123)
                     /locus_tag="ACHK49_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO9264694.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRDEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPPKPKKEKSSSREGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLARGRAELYPEMHGTVTGFKSEIDN
                     QDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETA"
     gene            1281..2465
                     /locus_tag="ACHK49_004159"
     CDS             1281..2465
                     /locus_tag="ACHK49_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO9264695.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAKLFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ASCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HDQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNGTTNLRQGRVRIRYKYTPVPPLEDMELYQTFTDEFFGPAFAVLG
                     GA"
     gene            2465..2977
                     /locus_tag="ACHK49_004160"
     CDS             2465..2977
                     /locus_tag="ACHK49_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO9264696.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTPPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRALFLKYGGTIDGTLLRFAGEYYTDAESDLYEIE
                     MRGRVTEIDMGEAKQGEATSHTYAVKNTYYKLSVNDRPLWEIDLLNHIYRKDGKDIVP
                     DRIRSALGLG"
     gene            3033..3407
                     /locus_tag="ACHK49_004161"
     CDS             3033..3407
                     /locus_tag="ACHK49_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9264697.1"
                     /translation="MKDIDTETRNNTVADDVTAGEDMAVERGVKLTRPIERGGEKITY
                     VEITGAIEQAGSLRGLSLSDVLNLKADTMFTLLPRVTSPRLDEVMIKKMSSRDFIQLC
                     AVAVNFMSEPDSGAKSVQETAA"
     gene            3416..3571
                     /locus_tag="ACHK49_004162"
     CDS             3416..3571
                     /locus_tag="ACHK49_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO9264698.1"
                     /translation="MCFEHIEDLVADIAAIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            3558..6365
                     /locus_tag="ACHK49_004163"
     CDS             3558..6365
                     /locus_tag="ACHK49_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9264699.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGILKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVSVSDA
                     GGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAVGDGSLPKLR
                     KELEGARGEAERTAKVMANNLDGDLKSLGSAWEGLRIRIADLIDGPLRSVTQWLTRVV
                     SRVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLNPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPAASRNREPAVSRFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            6378..6866
                     /locus_tag="ACHK49_004164"
     CDS             6378..6866
                     /locus_tag="ACHK49_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9264700.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSATSSVKG
                     LL"
     gene            7023..>7114
                     /locus_tag="ACHK49_004165"
     CDS             7023..>7114
                     /locus_tag="ACHK49_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFO9264701.1"
                     /translation="MLSYLMAIHFVLFGDSTNLKNFWKYEVIRR"
BASE COUNT         1610 a         1771 c         2076 g         1657 t
ORIGIN      
        1 acatttaaga cattcacgct gtttctgcta tccattccgg gatttttgcc tcaagctcaa
       61 gctgcgtggt aaagccgctg ttatcaatgg tgtgctcggc ttttgcaata atccagtcct
      121 gattatcaat ctcgctttta aatcctgtta ccgtgccatg catttcgggg tagagttctg
      181 cacgtccacg cgccagcgtg atggaaaatg atgcggctcc gcgttgtagc tgctgccact
      241 ttgccgccgc tgcgcgtctt gctgcctgct cgttctgata agtcttgcgt aacacaaaca
      301 cattgccttc cgcgccttcc atataatcac cttcacggct gctgcttttc tcctttttgg
      361 gttttggcgg tttgcggcgt ttcacgctga cttttttctt tttcccgtaa ttaagatcaa
      421 gccagtaagc gcgtaccccc gtatacgcct cgcggtcagc aatgcggaac tgatggcgat
      481 cgccgctgct gcgtgtgatg gcgaacgatg gcaacggctg gccctgtgcg ttcacgccac
      541 cacctggcat gatgaataac agattgccgc tttttaccgt ggtgattgcg cccagcattt
      601 ccgccatgcg cgtaaggaag gacatatcgc tttcttcggt ctggtcggcg tggtcgattt
      661 cgatatccat cagcatttcg ctgatttgcg gtttcagacc ataccgatga gcgatggcgg
      721 ataccacgcg ctcaacggtc acatcatgcc aggacacctc acgtttaacg ttaaattcat
      781 cccgaaaatc tgcgcttctg gctgaaacag tcagcctgtc cggcggtcct tcgtgagcga
      841 tttcatcaac aatgtaagtg cctttttctg tcagcggttc tcctttccag ccaatgagaa
      901 ccgtcaggcg cgcgccccgt ggcggtagct gcaactgacc atcagcatca tccagcgtga
      961 tggtgagctg gtccgcctca aatccccggt tgtcggtcag tgacaggctc atcaggcgct
     1021 ctgccacgcc tgacagcgtt ttaccctccg cgagaatatc aaaatccggc attttcacgg
     1081 ggtctgtgcc ctgactgagc aattgcatgg tggtgtcggt catctgttcc ctccctgtgc
     1141 ggcatggtcg catgtgcgtg cggagggggt tactgctttt tgttgtcgcc gtggtgggag
     1201 aacggcgcag gggtgagatt acgcgcgtgg tgggtgatga ttgttgccga atcatttaac
     1261 ggatacaagg ggctgaagct atgagtgaaa ctcgttttca tggtgcccgt gttacggaaa
     1321 ataccgacct ggtaacagcg attaacgatg ttgattccag cgttatcggt atcgtggcaa
     1381 cggcggatga tgcggacgcg aagctgttcc cgctgaacaa gcccacactg ctgacccgcg
     1441 ttaatgacgt gctgggaaaa tgcggaacaa cggggacgct ttatcgtgcg cttaaggcca
     1501 tcgcagacca ggtgagcaca aaggtgatcg tcgttcgcgt ggctgaacac aaagaagaag
     1561 acggaaagac gcaggatcaa ctggttatcg gtggttctga atctgacggc agctatacgg
     1621 ggatgtatgc gctgcttgtt gcagagcagg atgaaagcat cggataccgt ccgcgtattc
     1681 tggccgcgcc ggagctggac acggaggcgg taacaaaatc cctgtgcgtg attgcaggta
     1741 aactgcgcgc gtttgtgtat gcctcatgtc acggctgtaa cacgatggct gaggcgatta
     1801 cctaccgcca gaaattcaac gaacgtgagg tgatgctctt atggccggac ttcatcgcct
     1861 acaacccgaa aagtggcaaa aacgaaacgt tccccgcgcc tgcctatgcg tgcggccttc
     1921 gtgcgtacat tgaccatgat caggggtggc acaaatcact gtccaacgtt ccggttaaaa
     1981 atgtgctggg gatgtcgagg catgtgttct ggtcgttgca ggccgaagac agcgatgcca
     2041 acagcctcaa caacaaagaa atcacgacca ttattcgtcg caacgggttc cgcttctggg
     2101 gcaaccgcac accggaaacg aacgcctaca tctttgaggt gtatacccga accgcacagg
     2161 tgctggctga ttcaattgcg gaagcgcagt ttgaaactat cgacagtcca ctgacgcctg
     2221 cgaacgtgaa ggatgttatc agtgccatca gggcaaaact ggattcgctg gttactgccg
     2281 ggaaactgat tggcgcggag tgctggtatg acgtggtgga taacggcacc acgaatttac
     2341 gtcagggacg tgtgcgtatt cgctacaaat atacgcccgt tccgccactg gaagacatgg
     2401 agctttacca gacgtttact gatgaattct ttggtcccgc atttgcggtg ctgggaggtg
     2461 cctgatggct gtaccaaaac atcttcgctt ttttacgttg tttgtggatg gtgaaaacga
     2521 agtgggcaaa gtgacatccg tcacgccgcc caaactgacg cgcaaaaccg acagctatcg
     2581 tggtggtggc atgatggggg cggtaagtat tgatctcggt ctggacgact ccgcgcttga
     2641 tgcgagtttt gtcatggggg gagctgttcg tgcgctgttc cttaaatatg gtggcacgat
     2701 tgacggcacg ctgctgcgtt ttgcgggtga atactacacc gatgcagaaa gcgatctgta
     2761 tgaaatcgag atgcgcgggc gtgtgacgga aattgatatg ggggaagcca aacagggcga
     2821 agccacatca cacacttacg ctgtcaaaaa cacctactac aagctgagcg ttaacgatcg
     2881 cccgctgtgg gaaatcgacc tgttgaacca catctaccgg aaggacggca aggacattgt
     2941 gcctgaccgt atccgttccg cgcttgggct tggctgataa gtaatatgca ggcggcgcag
     3001 tgcgtcgcct ctgactgaaa aggagacaac tgatgaaaga catcgatact gaaacccgga
     3061 ataacaccgt ggcggatgat gtgacggcag gtgaggatat ggctgtcgaa cgtggcgtaa
     3121 aactgacccg accaattgag cgtggtggcg aaaaaatcac gtatgtggag atcaccgggg
     3181 ctattgagca ggctggatcc ctgcgtggtc tgtcgctgtc tgatgtgctg aatctgaaag
     3241 cggataccat gtttacgctg ttgcctcgcg tgacctcgcc acgactggat gaagtgatga
     3301 ttaaaaaaat gtcgtcacgc gattttattc agttgtgcgc tgtggctgta aattttatga
     3361 gcgagccaga ctctggcgcg aagagcgtgc aggagacggc agcgtaatca ccttggtgtg
     3421 ctttgagcac atcgaagatc tggtggcgga tattgccgcc atttttaact ggtcgcccgc
     3481 cgaaatcttc atgatgacgc ccggcgaagt ggttagctgg cgcgagcggg cggcacttcg
     3541 cagcgggaat gcagataatg aagactcttg atatccgggt cgctttcagc gccgttgaca
     3601 ggctgacccg gcctgccgaa aacgcccgcc gcctgatggg gcagtttggt gactccatcc
     3661 agcgaacgca gggggcgatc aaaaatctcg agcgtcaggc gcgttcattt gagcgcgccc
     3721 gtgacgctgt cagtaaagcg gatgctggca tattgaaagc acgacgccag cttaacgctc
     3781 ttaatcagtt acaacgcacg ggtacagtgc tcagcgaaaa acaacaaaag ctgatgcagc
     3841 agttaagcac ccggcttgaa cgcctgaatg aatcgcgcac acgggaaatt cagaaaatgc
     3901 gggaacttgg cggagagctg aaacgccacg gcatttccct gacaggcagc gataacacca
     3961 tccagcaggc catcagacgc accgaacaat acaacaacca gcttgaacgc gaacggcagg
     4021 cgcttgcgcg tgtaacgcgg gcgcgtgagc ggtattcgcg cgcgcaggaa accgcgggaa
     4081 aactgaaaac aggtggtgcg ctggcaattg gtgcggcagc ggcgggcggc tatgctgccg
     4141 ggcgtttttt gcagcccgcg atcgggttcg gcaaagagat gtcccgcgtt caggcgctga
     4201 cgcgaatcga ccagaacagc ccgcagttta aggcgctgcg tgagcaggcg ttaaaacttg
     4261 gctctgaaac gcagttcacc gcaggcgatg ccgccagtgg gcaggcattt cttgcaatgg
     4321 caggcttcac accgcaggcc attcaggctg cgcttcccgg agtgctgagc atggcaacgg
     4381 ctggcggtat ggatctcggc gagacggcag atattggctc aaatatcctg acgcagttcg
     4441 gcctttctgc tgaccagatg gaccgggtcg gtgacacact caccgcagcg tttacccgta
     4501 ccaacactga ccttcgcgca ctgggcgaaa ccatgaaata tgcaggtccg gtggcgggta
     4561 agctgggaat atcgctggag caggccgcag cgatggctgg tgtgctggcg aatatgggta
     4621 tcagagggag tgatgccggg acggcaatgc gtgccagcct ggctcgtctg gcatcaccgc
     4681 caaaggcggc ggcagaagct ctgaaagagc tgggcgtgtc cgtctcggat gccgggggta
     4741 aaatgcgccc gatggaggat gtgctggccg acctttataa agccacccgc aaatacgggg
     4801 aagttgaccg ggtatcgttc tttaaggaca ttgccggaga agaggctttc acatcgttta
     4861 tggcgctcgt tgatgcagtg ggtgacggtt ccttacccaa actgagaaaa gaacttgaag
     4921 gcgcgcgcgg tgaggctgaa cgcacagcaa aggttatggc caacaacctt gacggcgatc
     4981 tgaaatcact cggcagtgca tgggaagggt tgcgcatccg cattgcagat ctgattgacg
     5041 gtccgctgcg ttctgtcacg cagtggctca cgcgggtggt atcaagggtg acggcgctgg
     5101 cgcaggccca tccggcactg acgcgccagc tactgatagc aggcggtgcg ttgctggcaa
     5161 tgactgcaac ggttggctcg ttgtcgctgg ctattggtgt gcttgctggt ccgctggcaa
     5221 aactgcgtct tggtttttct ctcctgaccg gatcaatgaa tgctgtcagg gtcctgccag
     5281 cactatgggg aatggtgacg ggttccgttt ctttactggg aggcgctatc ggggcgctgt
     5341 tcagtccggt tggtcttatc gtggctgcgc ttgccggagc tgccgtcctt atctggaaat
     5401 actgggatcc catcagggca ttttttgccg gggtgttcag cgggattatg gaaaggctga
     5461 acccgttgcg tgaaaccttt gaacggtttg gccctgtttt tgacgcaatc ggaagcggga
     5521 tcagccaggt gtttaactgg tttaaatcgc tgctgtcacc gatggagtcc agcaaggaaa
     5581 cgctggataa atgtaccagt gctggcgaga tattcggtaa cgttcttggc ggtgcgttac
     5641 aacttgttct gacacctgca aaaatgctac tggatacgct ggcgtggata cttgaaaaac
     5701 ttggcgtcct tccggatgaa gcggaaaggg cgcgcaagaa aatcgaagac gcacagcgtg
     5761 cggccattct tcaggataag gttgccttac ttcaggggga cctggcgaaa atcaatccgc
     5821 cgaagcctgt ggaaaatggc aatggcaccg gaggtgataa acccaaagac aacaaaccgc
     5881 tcacagacag caataccggg acgctacgca gactcagcaa aattgctgat aacacaggta
     5941 agctggttga tgagacgaaa aaacgcattg gccctggcga tattgtcttt aagaacctgc
     6001 cccgcgcact tgctgttcgt ggggagtggc aggagcggaa gattgcgcag gtcagtaagc
     6061 ctgcccccgc aattaatatc acacccgtag tcccggctcc gctgcctccg gcgctggtcc
     6121 ctgttgttgc ggccagctcc cgcccggtgg cggaggccat acgatcgcca gtggcatcag
     6181 ttcctgcagc ttcccgtaat cgggagcctg ctgtctccag atttggcggt gaaattcatg
     6241 ttcatctgca taacgttgtt acgcagaatc cccgcgaact ggcgaaactg gtcggtgaaa
     6301 tggtcagggc agaaatggaa cggcgcgccc gtgccgggcg tggcagtttt tacgataaag
     6361 attgaggagt catggccatg atgatgatct atggcatgtt tgtttttgag ctgcgcacgc
     6421 tgccgcatca gcagttacag caaaacaaaa gctggcggca tgtgaaaaat gaacgcgtta
     6481 accgttcagc aagctggcag tatatcggtg caggtgatga tcgcatcgtt ctttctggtg
     6541 tgctttatcc tgaaattaca ggtggcgaag tgtcgctgtc tctgctgacc acgcaggcgt
     6601 atacaggacg gccctggcct ctgattgatg gcgtcgggca gatttacggc atgtatgtcc
     6661 tgaccgaaac gaatacgacc cgctccgagt ttgatcgcta cggtaaggcg aaaaagatag
     6721 aattttcact gactcttgaa cgctgtgatg aggatttgcg ggagcgcctg caatcctcat
     6781 cgttcagcga tatgttgtcc ggcttcaaag ataaggtgac atcatccctt aatagcgcga
     6841 ccagctcagt taaggggctg ctctgattta acgtatgtcg ccaatttcct gatgaaggtg
     6901 actggcgact tgctgttgta tgtccttctc agaaaattgt ttttgaataa caaacaacag
     6961 gattttataa tctcttaacc ttataatatg tgtggtctga aataataatt aaggagatta
     7021 tcgtgctgtc ttacttaatg gcaattcact ttgttttatt tggggactct actaatttaa
     7081 aaaacttctg gaaatatgaa gtaattcggc ggaa
//
LOCUS       DAZGLJ010000229         7071 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000229 DAZGLJ010000000
VERSION     DAZGLJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.229"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            81..1415
                     /gene="adeQ"
                     /locus_tag="ACHK49_004166"
                     /gene_synonym="yicO"
     CDS             81..1415
                     /gene="adeQ"
                     /locus_tag="ACHK49_004166"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO9264702.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1468..1920
                     /gene="yicN"
                     /locus_tag="ACHK49_004167"
     CDS             1468..1920
                     /gene="yicN"
                     /locus_tag="ACHK49_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO9264703.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2131..3321
                     /gene="nepI"
                     /locus_tag="ACHK49_004168"
     CDS             2131..3321
                     /gene="nepI"
                     /locus_tag="ACHK49_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO9264704.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3362..3655)
                     /gene="yicS"
                     /locus_tag="ACHK49_004169"
     CDS             complement(3362..3655)
                     /gene="yicS"
                     /locus_tag="ACHK49_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO9264705.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIHEAIVAMERNNQSNYWEALGKVECPDM"
     gene            3877..4695
                     /gene="nlpA"
                     /locus_tag="ACHK49_004170"
     CDS             3877..4695
                     /gene="nlpA"
                     /locus_tag="ACHK49_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO9264706.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4699..5622)
                     /gene="yicL"
                     /locus_tag="ACHK49_004171"
     CDS             complement(4699..5622)
                     /gene="yicL"
                     /locus_tag="ACHK49_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO9264707.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5733..6917)
                     /gene="setC"
                     /locus_tag="ACHK49_004172"
     CDS             complement(5733..6917)
                     /gene="setC"
                     /locus_tag="ACHK49_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFO9264708.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLGDE
                     LKARPIMVDFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMICIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1775 a         1591 c         1784 g         1921 t
ORIGIN      
        1 atgtgataat tatcactgaa ccgttgttgt catttgtttt acaaaagcaa gggattaaat
       61 ctcctccaat ggacaaaaaa atgaataatg acaataccga ttacgtgagt aatgaatcag
      121 ggacgctttc gcgattattt aaactacctc agcatgggac caccgtccgc acagaattga
      181 ttgcggggat gaccactttt ttaaccatgg tgtacatcgt ttttgtgaac ccgcaaatcc
      241 tcggcgcggc acaaatggac ccgaaagtgg tgtttgttac cacctgtttg attgccggta
      301 tcggcagtat tgcgatgggg atatttgcta acttacccgt ggcgctggct ccggcaatgg
      361 ggctgaacgc cttctttgcc ttcgtggtcg tgggggcgat gggcatctcc tggcagaccg
      421 ggatgggcgc aatattctgg ggcgcagttg gactattttt gctcacgctg tttcgtatcc
      481 ggtactggat gatctccaac attcccttaa gtttacgtat tggtatcacc agcggaattg
      541 gattatttat cgccttaatg ggattaaaaa atactggcgt tattgtcgcc aataaagaca
      601 cgctggtgat gattggcgat ttaagttctc acggcgtgtt gttaggtatt ttagggtttt
      661 ttattataac cgtgttgtca tcacgtcatt ttcatgccgc ggtgctggtt tctattgtgg
      721 tgacgtcttg ctgtggatta tttttcggtg atgttcattt tagcggcgtc tattccattc
      781 cgcctgatat tagcggcgtc attggtgaag tagatttgag cggcgcgtta acacttgaac
      841 tcgccggtat cattttctcc tttatgctga tcaacctatt tgattcatca ggaacattaa
      901 ttggtgtaac tgataaagcg ggcttaatag atagtaacgg taaattcccc aatatgaata
      961 aggcgctgta tgttgatagc gtcagttcgg tggcgggggc gtttatcggc acctcgtctg
     1021 ttactgccta tattgaaagt acttctggtg tggcagtcgg tggtcgcacg gggctgactg
     1081 cggttgtggt tggcgttatg ttcctgttag ttatgttctt ctcaccgctg gtggcgatgg
     1141 ttcctcctta cgcaaccgcc ggagcgttaa tctttgttgg cgtgctgatg acttcgagcc
     1201 tggcgcgcgt taactgggat gattttaccg aatcggtgcc tgcgtttatt accacggtga
     1261 tgatgccctt tactttctcg atcaccgaag ggattgcact cggctttatg tcgtactgca
     1321 tcatgaaagt atgcaccggg cgctggcgcg atctgaacct gtgtgtggtg gtggtcgcag
     1381 ctctgtttgc actgaagatt attctggtgg attagtaact ggcgagggtt tccagatatc
     1441 atgagttctg attacgcagg agaactcatg atctggataa tgctcgccac gctggcggta
     1501 gtgtttgtgg ttggttttcg ggtgctgaca tccggggcca gaaaagcgat tcgccgtctc
     1561 agcgatcggc tgaacatcga tgtcgtaccc gtggagtcga tggtcgatca aatgggaaag
     1621 tcagctggtg acgaattttt acgttatttg catcgtccgg atgagtcgca cctgcaaaac
     1681 gccgcgcagg tgttgctcat ctggcaaatt gtcattgtcg atggtagcga acagaacctg
     1741 ctgcaatggc atcggatttt acaaaaagct cgcctcgccg cgccgattac cgacgctcag
     1801 gtcaggctgg cgctaggttt tctgcgcgaa accgaacctg aaatgcagga tattaatgct
     1861 tttcagatgc gctataacgc gttctttcag cctgccgagg gcgttcactg gttgcattga
     1921 aaccgtttcg tcttatcagc ctacagaccg cgcctgacct gtcgggtgga taagacgttc
     1981 acgccgcatc cggtaatctt tgccctgttc cgtttcgatc ttaactccag ctcaagcaac
     2041 accgtaaaag ttgcaaaaac gttaaatgcg tcacacattt caatgttaaa gtggtcggct
     2101 tttcccctga aacatgccac gggtaacacc atgagtgaat ttattgccga aaaccgcggc
     2161 gcggatgcca tcacccgacc gaactggtca gccgttttct cggtggcgtt ttgtgtcgcc
     2221 tgtctgatta tcgttgagtt tttgcccgtc agtttgttga cgccaatggc ccaggattta
     2281 ggcatttcgg aaggggttgc cgggcaatcg gtgaccgtga ccgcctttgt ggcaatgttt
     2341 gccagtttgt ttattaccca gacaattcag gctactgacc gccgctacgt tgttattttg
     2401 tttgccgttt tgctgacgct ctcctgcttg ctggtttcct ttgctaactc attcagtttg
     2461 cttttaatcg gtcgtgcctg tctggggctg gcgctgggcg ggttctgggc gatgtcggcg
     2521 tcgctgacca tgcgtctggt gccgccgcgt acggtgccga aggcgctgtc ggtgatcttc
     2581 ggcgcggttt ctattgcgct ggtgattgcc gcgccgttgg gcagtttttt aggcgagctt
     2641 atcggttggc gcaatgtctt taatgcggcg gcggtgatgg gcgtgctgtg tattttctgg
     2701 attatcaaat cattgccttc actgccaggc gaaccctcgc atcagaaaca aaatactttc
     2761 cgcttattac aacgtccggg tgtgatggca gggatgatcg ccatcttcat gtctttcgcc
     2821 gggcagtttg ctttcttcac atatattcgc ccggtgtata tgaacctggc gggattcagc
     2881 gtggatggct taacgctggt gctgttgagt tttggcattg ccagctttat cggtacgtcg
     2941 ctttcgtcat tcattcttaa acgttcggta aaactggcct tagcaggcgc gccgttaata
     3001 ctggctgtga gtgcgttggt gctgacgttg tggggaagcg ataaaatcgt tgctaccggc
     3061 gtggcgatta tctgggggct aacttttgca ttggttcccg tcggctggtc aacgtggatc
     3121 acccgctcgc tggccgatca ggcagaaaaa gccgggtcta ttcaggtggc ggttattcag
     3181 cttgctaata cctgtggcgc ggcaatcggc ggttatgcgc tggataatat tggtctgact
     3241 tcgccgttga tgttgtccgg cacattgatg ttgctgactg cattgttggt tactgcaaag
     3301 gtgaaaatga agaaatcctg atatcaggta aagaagcgtt ggtcagtagt tgttgctgtt
     3361 cttacatatc cgggcattct accttaccca acgcttccca ataattgctc tgattattac
     3421 gttccatcgc cacgatggcc tcgtgtatat gttgtttatt gttttcatct gacaacatta
     3481 acttttccca cgcttcatct gataatgacg cctgtggcgt gcaaatttta cgtaaatctt
     3541 gtaataccgt ggttttctct tttgcggact gcagtgagct aaaaggcgac tcggcgtata
     3601 cgatgcctgg catagcgaaa aaagtgaaaa taagcagtag tgtcgttggt ttcatggatt
     3661 caacctttta caaatgtgta tcgaatgaag cataagtgta gtttgctttc atcgctctca
     3721 tcatgctgta cgaaacgcgc ttgcgtggtc agtaagaagt gccagacttt atattccact
     3781 tttattcctt tttattctta tcgatagcgt ttcgtttttt aaaccgcagc gaccttaccg
     3841 ctatagtcag gtaatcatta ataaaaggat aaaaaaatga aactgacaac acatcatcta
     3901 cggacagggg ccgcattatt gctggccgga attctgctgg caggttgcga ccagagtagc
     3961 agcgatgcaa aacacattaa agttggcgtt ataaatggcg cagaacaaga tgtcgcggaa
     4021 gtcgctaaaa aggtggcaaa agagaaatat ggcctcgatg ttgaactggt aggatttagc
     4081 ggttcgttgc taccgaacga tgcgacaaat catggcgaac ttgatgcgaa tgtcttccag
     4141 catcgaccct ttcttgaaca agataatcag gcgcatggtt ataaactggt agcggtggga
     4201 aatacgtttg tcttccctat ggcgggttat tccaaaaaaa tcaaaaccgt ggcgcaaata
     4261 aaagaaggcg cgacagtggc gatccccaat gacccgacca accttggtcg cgcactttta
     4321 ctgttgcaaa aagagaagct aattactctg aaagagggaa aagggttatt acctaccgcg
     4381 ctggatataa ccgataatcc tcgtcatttg cagattatgg aactcgaggg ggcgcagctc
     4441 ccgcgcgtac tggatgatcc caaagttgat gtagcgatta tcagcaccac ttacattcag
     4501 cagacagggc tttctccggt gcacgacagc gtatttattg aagataagaa ttcgccgtat
     4561 gtgaatattt tggtggcacg ggaagataat aagaatgcag aaaacgtgaa ggaatttctg
     4621 caatcttatc aatcacccga agtcgctaaa gcggcagaaa ccatttttaa cggtggcgcg
     4681 gtgcctggct ggtaattctc actcatgccg cgccggacga tttatttttc tggcgcggcg
     4741 acgggaatcc attgaaatca aaattaccga tgacagaatt agcagcgttc ccagccagtc
     4801 cggtaatgtg aacgtgatcc ccagcaacag caaagagagt agcgcgctac ttaatggttc
     4861 tgcacagctc aaaatgctgg cttttggacc gccaattaat tgtgctcctt tcaggtacag
     4921 actaaatgtc agggacgtac caatgacgat caaataaaaa aacgccagaa tcaaactgcc
     4981 gttaacgaca aagtttgttc cttgtctggc ataaaaaggc aacagaatca gaccgccaat
     5041 cagcatactc cagccgacga ctggtaacgt gccatagcgg gcaattagtg ttgagggata
     5101 ggtggtatag aatgcagcag caaatgccga ggcaatgccc cagaacaacg cggcaggaga
     5161 gatcgataat gacgtcggat taccgtgtgt caccaataaa aaagtcccga ccagcgatgt
     5221 caaaatagcg cagaaaacca gaatgcccgg gcgcgattta cgcaccagtg agaaccaggc
     5281 gacgataatc gtcggtgaga ggaattgcag caccgttgcc gtggctgcgt tcgatttttc
     5341 gatggttagc aaaaaagtga gctgtacagt tagcgcgcca accacggaaa aaatcagcag
     5401 gctaatggca tctttatgat tgttaataat agaaaagatt ttatcgccat gaacaaatga
     5461 cagcgtcagt agaatcaaac cggcgaatat caaacgcgtc atagtcaaaa actgcgacga
     5521 catctggctt tgctccatga tgtattgcgc acagacccct gaacttcccc acaacacggc
     5581 ggcaatcaga acgttcagca tcccctttct ggtggaaccc atttttccct ctgcatactg
     5641 ttttgtcgtt tatgtatgca ggcatgatag caaaaatggc gaggatggct ctgtttgcgc
     5701 cacgccgatt tttcttagcc cagactggtg agctaaatat ctttaataaa cagcaggcaa
     5761 atcatcgcaa tacccaacat ccctatcgcc agccagaaca acgcatggta gctccagatt
     5821 tcaaccataa ttccgtcaac agagccggcg ataatccagc cgacgcgtga agtatttgca
     5881 tataacgttg tcgcagagcc tattttttca ggcatcaagt cctgaaaata aagcatgccg
     5941 ataccacaga gaataccaag gaagatggca ttaagaattt gcaattccag ctcaaccgcc
     6001 ggagtcgtcg ccatgagtac gctggcgtaa aaacacattc cactcacgat agcaatgagc
     6061 attaatagtc gcttgccaat acgtttcatg taatagcctg cgattaacat catcggaatt
     6121 tccagaccgg cagcgataca aatcatttct ccagccagtt tatcggttag atgcagttca
     6181 tcaataataa atagcggcat atttatcatg tagagattat tcgccgccca catcattgaa
     6241 cagaccacaa aaagtagccg cgtatcccgc ttcctgtggg tggagggtaa aatttctacg
     6301 ggttgggtaa cgacaggaat atttctttgt attgatggca aaaacaacca gactatcagt
     6361 ccgcaaacaa caaatgcgat ggcagcggtg agatacatca ctttaaaact aaatcccatt
     6421 gccagttcat aagcgagcgg tggcccgata acccaggcaa gcgagatctg agcacgtaaa
     6481 aatgtactga acatgaccgt ctcacggcct gttctgtcgg cgtgttcacg ggcgagggcg
     6541 aacatttgcg ggtttgcggt ggaagcaaaa ctactcagaa gtacgcccgt tgagaggaga
     6601 atgaagtagt tgcgattcca cgcaaaaagc gtgcaggcca gcactccaaa taagcaacat
     6661 agcagaatca gtaatttacg gtcgccttgt ttatcggagt gccttgccag aaattgactg
     6721 accagaattc ccataatagc gctaccggtg aagaaaaaat ctaccattat aggacgggct
     6781 ttcagttcat ctccgaggaa tatacttagg gtaggagtct gaagagcgcc cgcaataccc
     6841 gtcagaaagg cgacaagtaa aaatgccgcg gcagtgagat caagtatttt tgatggagtg
     6901 gtagccgttt tttgcattct tctgaatcac cgggcgacaa aatataactg agttacaaat
     6961 aagtaccagt cgaatgagta ttgtattaag aatttttgag tatcgctgat acaaccagaa
     7021 agcaatgcta gcgcaatatt atagggccag caattgagat tattagcaaa a
//
LOCUS       DAZGLJ010000230         7063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000230 DAZGLJ010000000
VERSION     DAZGLJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.230"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..6292
                     /gene="efa1"
                     /locus_tag="ACHK49_004173"
     CDS             <1..6292
                     /gene="efa1"
                     /locus_tag="ACHK49_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lymphostatin Efa1/LifA"
                     /protein_id="HFO9264709.1"
                     /translation="IHNKIQRIKDEFKEYSRVTIHNIDKVIFKGQTLDRLYHEGYVFS
                     DINTLSRYTLHGLGITGVHTEENLLPAPSSSLINILKEHYNEDEISAKLPLAYDYILN
                     KKESSSIPVEILNKLSELPPHELLTPVLGQSVNPLGMGYSSDNGKITEQVIVSGADGF
                     DNPISGLIYTYLEDLYNIHVRMREGTLNSQNLRQLLENSVSSCFLTEQSINKLLSEAE
                     KRPYQSLTEIHQHLTGLPTIADATLSLLSVGLPGTGKLLRREQDYGRPPVTAIQDSTF
                     VLPYNFKGIGFNDNIISSAPVVSSLHFIAEHAKYTLLSWPEFYRHHAQRWFEMAKGYG
                     SQNIDFHPQSLLVTQEGRCMGLALLYLQTEDTAHYSILQENLMTVSALHQTSNRDKLP
                     LSKDDNSLMTRTYSLIEMLQYQGNKYITNESLLHKTAWNQERITLLFNEKGVKRALIS
                     TPNHTLVLQQLEDIYRLTDPNFGHADFLSPIDALKFIEAMIQLTPTLQEYYGLLNKDI
                     NKHIQVHYAESDMVWNKLLPENDAGLSTRIQHTTTDRLANLAEPVAVAGISLPVKTLY
                     DIGATLDGRRITSPPTSEQIPSLRLNGDVLNDYLSRTVLTPEQADNIRKILHTQGIRS
                     GTRPIDPEMIRGTQDDLVSSQTRLQRQATRVKQQLAGVLDTLQQHFQNIPRSSGRHLS
                     VENIELADIGSGRFNLQIRDGETLHTTSVEVPEVVSRFQKLSTMLSALPASGIMDFDL
                     GMSVVGVVQYARLLQQGHEDSTLAKINLAMDIKQLSEATLGSMIQIAGNKFLNTEGIQ
                     GFRLESAVAEGMRSVATRTGGTMGKALSASARVLELPVLETVLGTWNLYNSVIQLQQA
                     TSYSETMAARVQIAFDSISLGLTAASVAFPPLIIATGPIAAIGMGASSIARNVARKEE
                     RHTQWLEYKKFLTDGSKHIVVASPERGLLDFSGNKVFGKMVLDLRQSPPLLHGESSFN
                     ADRKIGHRPDLGDWQIREKVGYANSISPYSSLAHGYANSKWPQTIPKIPSGEYDTIIL
                     GYGHQYQANTEIEYLSNWIVWREAVPDSTSRHKRPPLEVLNSQCTVIAGERKTTVLPL
                     RVLSDLTPECTEQAISLKDYKFILRGGSGGLAVQVGGAGYYDIDANPVAKENTLSFRG
                     LPEEFPLAFDLSKQTQSVMLKTTDGEVPVMTITQKGINTLVGTTAGENWLTGNDKDNT
                     FHTSSGGGTVISGGGNNRYIIPRDLKTPLTLTLSNSSVSHEILLPETTLTELKPVAFE
                     LSLIYWAGNNIKVQLEDETQLNRFAGNFRGHTSDGITLEAVSQENGIQLGVSSCDVQR
                     WQAVYPEENNRPDAILDRLHDMGWSLAPEVRFRGGETSASYDPLNRQLVYRLQERYSE
                     FRLTGSRHYTTAVTGTPGSRYIITGPDTAQISPAQIILAGDNDHPETIDLLEASPVLV
                     EGKKDKNSVILTIATIQYSLQLTISGIDESLPGTTRVAIQPQDTRLLGDVLRILPDNG
                     NWVGIFRSGHTPTVNRLENLMALNQVMTFLPRVSGSAEQVLCLENLGGVRKKVEGELL
                     SGKLKGAWKAEGEPTVPVNISDLSIPPYSRLYLIFEGKNNVLLRSKVHAAPLKITSAG
                     EMQLSERQWQQQEHIIVKPDNEAPSLILSEFRRFTISSDKKFSLKLMCHQGMVRIDRR
                     SLSVRLFYLREQPGIGSLRLTFRDFFTEVMDTTDREILEKELRPILIGDTHRFINAAY
                     KNHLNIQLGDGVLNLADIVAEYARIQKEETSKILYQYQGAMKKKTDGPSVVEDAIMTT
                     TVTTDSGEPFPTFHPWYTDDLSGRYKSVPMARKADTLYHLTPKGDLQIIYQVATKMVN
                     QAMIVSLPNYRHEWEKYNLSILSEIPQNNNTVVHSILRVNGPTMQVRTIDYRGTDENN
                     PIVSFSDTTFINGEQMLSYDSHSSGRVYSREEYMMWELQQRVSEASSARTQDYWLMDA
                     AVRNGEWKITPELLRHTPGYIRSTVSKWSRGWLKTGTILQTPEDRNTDVYLTTIQNNV
                     FSRQGGGYQVYYRIDGMAGADIADNAPGETRCTLRPGTCFEVTSVDERHYEWNIIYVT
                     LKTCGWSRNGQSKTPNGDNLFN"
     gene            6464..6814
                     /locus_tag="ACHK49_004174"
                     /pseudo
     CDS             6464..6814
                     /locus_tag="ACHK49_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2116 a         1590 c         1606 g         1751 t
ORIGIN      
        1 tattcataat aaaatacaaa gaataaaaga cgagttcaag gaatactccc gtgtaaccat
       61 tcataatata gataaagtta tatttaaagg gcaaacactg gatcgtcttt atcatgaagg
      121 atatgtattt tctgatatca ataccttgtc tcgttataca ctacacggac taggaataac
      181 tggtgtacat actgaagaaa acctgctacc agctccttca tcgtccttaa ttaatatatt
      241 gaaagaacat tataatgaag atgaaattag tgcgaaatta ccactagcat atgattacat
      301 tttaaataaa aaagaatcaa gctctattcc tgttgaaatt ttgaacaaac tttcagagtt
      361 accaccacat gaactactca cacctgttct tggccagagt gttaatcctc tgggcatggg
      421 ctactcatcc gataatggaa aaatcacaga gcaagtaata gtcagtggag ctgatggatt
      481 tgataatccc atatctggac ttatatatac ctatcttgaa gatctatata acatccatgt
      541 aaggatgcga gaaggtacac taaattcaca gaatcttcgt cagcttctgg aaaactctgt
      601 ttcttcatgc tttttgactg agcaaagtat taataaatta cttagtgagg cagaaaaaag
      661 gccttatcag tctttaacag aaatacatca gcatctcaca ggattaccaa ctattgccga
      721 tgcaaccctt tcattacttt ctgttggatt acctggtacg ggaaaactat tgcgcaggga
      781 gcaggactat gggcgtccac cagttacagc aattcaggat tccacatttg tactccctta
      841 taatttcaaa ggtattggtt ttaacgataa cattatatcc tctgcacctg tagtctcctc
      901 attacatttt atcgctgaac atgcgaaata tactttgttg tcatggcctg agttttatcg
      961 tcatcatgca cagcgatggt tcgaaatggc taaaggatat ggaagccaga atattgattt
     1021 tcaccctcag tctctattgg taacccaaga aggacgctgt atgggattag ccttacttta
     1081 tttacagact gaagatactg ctcattatag cattctccag gaaaacctaa tgactgtgag
     1141 tgcacttcat cagaccagta atcgcgataa gttgccactg tccaaagatg ataattcctt
     1201 aatgacaaga acttatagtc tgattgaaat gctacagtat cagggaaaca aatatattac
     1261 caacgaatcg ctactacata agaccgcatg gaaccaagaa agaataactt tattattcaa
     1321 tgaaaaagga gttaagcgag ccctgataag cacgcctaat catactctgg ttctgcaaca
     1381 actggaggat atttaccggc tcactgatcc aaattttggg catgcagatt tcctttcacc
     1441 tatagatgct ctgaagttta ttgaggctat gatacaatta actccaacac ttcaggaata
     1501 ttatggccta ttaaacaaag acattaataa acatatacaa gtacattatg ccgaatcaga
     1561 tatggtctgg aataagcttc tgccagaaaa tgatgctgga ctgagcacca gaattcagca
     1621 caccaccacc gaccgtctgg cgaatctggc tgaaccagtc gctgttgcag gtatctccct
     1681 gccagtaaaa acactttatg atatcggagc cacccttgac ggtcggcgca tcacctctcc
     1741 tccaacatcg gagcaaatcc cttctctgcg tctcaacggt gatgttctga atgattatct
     1801 gtcccgcaca gttctgactc cagaacaggc tgataacata agaaaaatac tgcacactca
     1861 gggaatacgc agcggtaccc gtcccataga tccggagatg attcgtggga cgcaggatga
     1921 cctagtttcg tcacagactc gtctgcaaag gcaagcaaca cgggttaaac agcaactcgc
     1981 cggcgtactt gatactctgc aacagcactt ccagaacatt ccacgttcat ccggtcgtca
     2041 tctttctgta gagaatattg agctggctga tatcggaagt gggcgtttca accttcaaat
     2101 tcgagatgga gaaacattgc ataccacttc tgtggaagta ccggaagttg tgtcccgttt
     2161 tcagaaactt tctaccatgc tttcagccct gcctgccagt ggaatcatgg atttcgacct
     2221 cggcatgagt gtggtcggag tcgtccagta tgcccgcctg ctacagcagg ggcacgaaga
     2281 cagtacccta gccaagataa atctagctat ggatatcaag caactttccg aagcaactct
     2341 cggcagcatg attcagattg ccgggaataa gtttctcaat acagaaggaa tccaggggtt
     2401 cagactggaa agcgccgtcg ctgaaggtat gcgctcagta gcaacccgta ccggaggcac
     2461 aatggggaaa gccctttctg ccagtgcccg tgttcttgaa ctgcctgtac tggaaacagt
     2521 tctggggaca tggaacctgt acaacagcgt cattcagctc cagcaagcta catcttattc
     2581 tgagacaatg gctgcccggg tacagattgc gtttgattcc atttctctgg gattaactgc
     2641 cgcttcggta gctttcccgc cactaattat tgccactggc cccattgcgg ctattggtat
     2701 gggagcttcc agtattgcac gtaatgtggc acggaaagaa gaacggcata cacaatggct
     2761 ggaatataaa aaattcctga ctgatggcag taaacacatt gttgtggcct ctccggaaag
     2821 aggtctgctg gatttctccg gaaacaaagt ttttggaaaa atggtgctgg atctgcgtca
     2881 gtctcctcct ctcttgcatg gagaaagctc ttttaacgct gaccgcaaaa tcggtcatcg
     2941 tccggatctg ggggactggc aaattcgtga gaaggtgggg tatgccaaca gtatcagtcc
     3001 ctactcttcc ctggcgcacg gttacgccaa cagtaaatgg ccacaaacaa taccgaaaat
     3061 tccctcggga gaatatgaca ccataattct gggctacggt caccagtatc aggccaatac
     3121 ggaaatagaa tatctgtcta actggattgt atggcgggaa gccgtaccag acagtacttc
     3181 ccgccacaaa cgtcctcctc tggaggttct taatagtcag tgtactgtga tagctggaga
     3241 gcgtaaaacc acagtacttc ccctgagagt gctcagcgat ctgacaccgg aatgcacaga
     3301 acaggctata tcgttaaaag attataaatt catactgaga gggggaagcg gtgggctggc
     3361 tgttcaggtc ggtggcgcgg gatattatga tattgatgca aatcctgtgg caaaagaaaa
     3421 tacgctctct tttcgcgggc taccggaaga gtttccgctc gcctttgatt tatcaaaaca
     3481 aacacagtcg gtcatgctga aaacaacgga cggtgaggtg ccggtaatga ccatcaccca
     3541 gaagggaata aacaccctgg tcggtacaac ggccggcgaa aactggctaa cgggtaacga
     3601 taaggacaat accttccata cgagttctgg tggcggtact gtcatctctg gtggagggaa
     3661 taaccgctat attatccccc gcgatttaaa aactccgctg acactgacgc tgtctaatag
     3721 ctcggtctct cacgagatcc ttctgccaga aacaactctg acagaattaa aacctgtcgc
     3781 ctttgagctg agtctgattt actgggccgg gaacaatata aaggttcagc tggaggatga
     3841 gacacaactg aaccgttttg ccggaaactt cagggggcat accagtgatg gcattactct
     3901 ggaggcggtt tcccaggaaa atggtattca actgggggtt tcatcatgtg atgttcaacg
     3961 ctggcaggct gtttatccgg aagaaaacaa cagaccagac gccatactgg acaggctgca
     4021 tgatatggga tggagcctgg cccccgaggt caggttccgg ggaggagaaa catctgccag
     4081 ctatgacccc ctgaaccgtc agctcgttta ccggcttcag gagcgttact cggaattcag
     4141 gctgaccggc agtcgccatt acaccacggc tgtaaccgga acccccggaa gccggtacat
     4201 tattacgggg ccggatacag cacagatatc accggcacaa atcatactgg ccggtgataa
     4261 tgaccatccg gaaacgattg atttgcttga ggccagtcct gttctggttg aagggaaaaa
     4321 agacaaaaac agcgtgatat taacgattgc tacgattcag tattcccttc aactgacaat
     4381 atccgggatc gacgaatcgc tgcccgggac aacccgtgtg gcaattcagc ctcaggatac
     4441 ccgtttactg ggtgacgtac tccggatctt accagataat ggtaactggg tggggatttt
     4501 ccggagtggt catacaccaa cggtaaaccg gctggaaaat ttgatggcac tgaatcaggt
     4561 aatgacgttc ctgccccggg tatccggaag tgcagagcag gtattatgcc tcgaaaacct
     4621 aggtggcgta aggaaaaaag tggaggggga gttactgtca gggaagctga aaggtgcgtg
     4681 gaaagccgaa ggtgaaccta ctgttccggt aaatatctca gatctaagta tcccacccta
     4741 ttcacgtctg tatctgattt ttgaagggaa aaataatgtg ttgctacgca gtaaagtaca
     4801 tgcagctccg ttgaaaataa catccgcagg agagatgcag ttatctgaaa ggcagtggca
     4861 acagcaggaa catattattg tcaagcccga caacgaagcc ccctcattaa tactcagtga
     4921 atttcgtcgt ttcactattt catcggataa aaaattttct ttaaaactga tgtgccatca
     4981 ggggatggtc cgcatcgacc gcagatcatt atcggtcaga ttgttctatc tgcgtgaaca
     5041 gccaggtatc ggcagtttac gtctgacgtt cagagatttt ttcacagaag tgatggatac
     5101 aacggacagg gaaattctgg agaaagagct gagaccaatt ctgataggag atacacaccg
     5161 ctttatcaac gctgcataca aaaatcatct gaatatccag ttaggagatg gcgttctgaa
     5221 tctggcagac attgttgcgg aatacgcccg tattcaaaag gaagaaacat caaaaatatt
     5281 gtatcaatat caaggtgcca tgaaaaaaaa aacagatgga ccatctgtgg tagaagatgc
     5341 cattatgacc actactgtca caacagattc aggtgaacca ttccctacct tccacccgtg
     5401 gtatacagat gatttatcag ggcgttataa gagcgtacct atggcaagaa aagcagatac
     5461 tttgtatcac ctgacaccaa aaggtgatct acagataata tatcaggtag ctacaaaaat
     5521 ggtgaatcag gcgatgattg tatccctgcc aaactaccga cacgagtggg aaaaatataa
     5581 tttaagcatc ttatccgaaa tccctcagaa caataatact gttgtacatt caatcctcag
     5641 ggttaatggc cccacaatgc aggtgcgcac aattgactac agaggaacgg atgaaaacaa
     5701 tcccatagta tctttttcag atacaacctt catcaatggt gaacagatgt tgagttatga
     5761 ctcgcattca tcagggcgag tctattccag agaagaatat atgatgtggg aattgcagca
     5821 acgggtatca gaagcttcca gtgcccggac acaggattac tggctgatgg atgcagcggt
     5881 aagaaacgga gaatggaaga tcacaccaga attattacgt cacacaccgg gatatatccg
     5941 gagtacggta tcgaaatggt ccagaggatg gctgaaaacc ggcacaatac tccagactcc
     6001 agaagacaga aatacggatg tatacctgac taccatacag aacaatgtat ttagtcgtca
     6061 ggggggcggc taccaagtgt attatcggat tgatggaatg gctggtgcgg atatagcgga
     6121 taatgcacca ggggaaaccc gctgcaccct caggcccgga acatgttttg aagtgacaag
     6181 tgtggatgaa aggcattatg agtggaatat catttatgtc acgctgaaaa cctgtggctg
     6241 gagccgaaat ggccaaagca aaacgccgaa tggtgacaac ctttttaact aattaaaatc
     6301 tctgttaaag atgtataacc cggcatctat gtgctcacca gcatatagat gcaatgtgat
     6361 tcccctgatg gtcgtatcag agatggtatg cgagcctgta catagatttg tgtaattgcc
     6421 tgattttgat atgttcaatc caacatcaaa agcaggttaa tttatggacg aaaaacagtt
     6481 gcaggctctg gctaacgaac aggccaaaaa tctcaaaacc cctgaagatc tcagccagtt
     6541 cgatcgcctg ctgaagaaaa tcagcgttga ggcagctctc aacgctgaaa tgtcccacca
     6601 tctgggctac gataaaaacc agcctaaacc gggtgccaac tcccgcaatg gctattccac
     6661 aaagaccgtt atcaccggtg atggccccct ggaactacgc actccgcgcg atcgtgatgg
     6721 ctcttttgaa ccgtaacttg tgaagaaaaa ccagacccgg atcaccggga tggataacca
     6781 gatcctgtcg ttgtacgcca aaggcctgac aaccgtaagc gtacagcctg aaccgtctgg
     6841 tcagaatctg acgaattaga gtaagctgga catccccaga tatattatgg ctgccagcgt
     6901 catatcggca taaatagaga tatcccttaa cgctaaccag aaaaaaatac ctataagagc
     6961 ctgtacatag agttgtgtcg ttgcctgatg ttgatatgtc aaatccaaca tcaaatagcg
     7021 gtaaatttat ggataaaaaa ttacaggctc tggttaacga aac
//
LOCUS       DAZGLJ010000231         7051 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000231 DAZGLJ010000000
VERSION     DAZGLJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.231"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(34..897)
                     /locus_tag="ACHK49_004175"
     CDS             complement(34..897)
                     /locus_tag="ACHK49_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO9264710.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAVFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(869..2419)
                     /locus_tag="ACHK49_004176"
     CDS             complement(869..2419)
                     /locus_tag="ACHK49_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO9264711.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            2677..3456
                     /gene="deoC"
                     /locus_tag="ACHK49_004177"
     CDS             2677..3456
                     /gene="deoC"
                     /locus_tag="ACHK49_004177"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO9264712.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            3583..4905
                     /gene="deoA"
                     /locus_tag="ACHK49_004178"
     CDS             3583..4905
                     /gene="deoA"
                     /locus_tag="ACHK49_004178"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO9264713.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            4957..6180
                     /gene="deoB"
                     /locus_tag="ACHK49_004179"
     CDS             4957..6180
                     /gene="deoB"
                     /locus_tag="ACHK49_004179"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO9264714.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            6260..6979
                     /gene="deoD"
                     /locus_tag="ACHK49_004180"
     CDS             6260..6979
                     /gene="deoD"
                     /locus_tag="ACHK49_004180"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO9264715.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         1644 a         1861 c         1932 g         1614 t
ORIGIN      
        1 gtctgtgccg aaccgtaggc cgtatccggc atgtcacaaa tagagcgccg gaaatatcaa
       61 ccggctcacc ccgcgcacct ttaacgcatc agccaacggc tcaacgtctt ccggcgtggc
      121 gctcgcccag ctttgcgcct cgccatacac gccgtgggca tgaaacgcgt tcaggcgtac
      181 cggaacatcg ccaagtccct tgataaacac cgccagttct tcgatgtgtt gcaaataatc
      241 cacctggcca gggattacca gcaaacgcag ttccgccagc ttaccgcgct ctgccagcaa
      301 acagatgctg cgcttaatct gctgattatc gcgtccggtt aattgttgat gacattcgct
      361 cccccacgct ttgagatcga gcattgcgcc gtcacacacc gggagtaatt tttcccagcc
      421 ggtttcgctc aacataccgt tactgtccac gaggcaggtg aggtggcgca gttgcggatc
      481 gtttttgatg gctgtaaaca gcgccaccac aaacggcagc tgggtcgtgg cttcgccgcc
      541 gcttaccgtt atcccttcga taaacagcac cgctttgcgg atatggctaa gcacctcatc
      601 cacgctcatg gattgcgcca ttggcgtggc atgttgcgga cacatcttca ggcaggtatc
      661 acactgctcg cagaccacag cgttccacag cactttgccg tcaacaatct gcaacgcctg
      721 atgcggacac tgtggcacgc actctccgca atcattgcag cgtcccatcg tccacggatt
      781 gtgacagttt ttgcagcgca gattgcagcc ctgcaaaaac agagccagac gactgcctgg
      841 cccgtcaacg caggagaagg ggataatctt actgactaaa gcgcatctgc tgttcatggc
      901 ttatcacgcg cggctggcgt tccagaatac gagtgttgcg tgcggcttct tcgcccagcc
      961 aggtggtgtt ggtgcgtgaa ccttcggcgc gatatttttc taaatccgac aaacgcacca
     1021 tataaccggt aacgcgaacc agatcgttac cgctgacatt ggcggtaaat tcacgcattc
     1081 cggctttaaa ggcaccgagg caaagctgta ccagtgcctg cgggttacgt ttgatggttt
     1141 cgtcgagggt cagaatgtcg ctgatgccgg aataataata agcatgatgc ggggcgacag
     1201 tttgcagatg ggtgatcgga tctggctcat cgccatacgg caaacgcgcg cccggcgtgg
     1261 tgccgatatc ggaactgatc cccgactgtg cgtgtaacat ggcgcgtttt tgccagccat
     1321 atttcacggg ggtattggcg acaaactccg ccagttgcgc gctgatgcga taacccactt
     1381 catttgcggc ggcttcttta ccgtagcgcg cggcaatccc ttctttctca cacagcaagt
     1441 taaccgcttc cgccagccca tacatgccaa acattggcac aaaacgttca gggttaatca
     1501 gcccttcttt caccaggaag ctattctcaa agaagtgtga ttgttgatag aggaattcac
     1561 accgcgcatc gatgatggcg atctgctgct ggcagtagtg cggtagagtg cgcgtaaaga
     1621 agtcatccag cgattcgctg cgctcggcaa tggctttcag gttaaggcgt accagcgtgc
     1681 tgccaccacc cgccagcggc agtgagttgt aacagctcac aatcccgtag cccccttttg
     1741 tgaaaatttt atcatgcacc ggaccgttgg cgatgtgcgg tttgctacat tcacagatgt
     1801 tcttcgccac ttccagcagc aggtcatcag gggtgatttc tggatcgtag ataaaggtca
     1861 ggttcggtga aacctgcttc aactctgcat ctgcacgtaa tatcgcacgg gtgatgggcg
     1921 aatcagacgg gccgatattg gcgtgcataa aggcgtctgg cagggttctg tcgaggtaac
     1981 gccagaaacg ttttattcga atatcgatct cgtcttgtgt tagaattcta acatacggtt
     2041 gcaacaacgc atccagttgc cccaggtaga ccggcatcga tgtgaccgac ggtacgtggt
     2101 ggtaaagaat ggtcagcaga gagagtgcgt catcaagatc tttcgcgcct tccagctcca
     2161 gccattcgga accgttcgcc agaaaacggg cgtaatcggg taagacatag cgcggtttgt
     2221 acggcgcatg accttcaaac atatcgcaga ttacaccctc atccagcgcg cggcgggcct
     2281 cggcaggcag ctgcggataa ggcagattgt tttctgcttc cagtgccaga aaatggcgct
     2341 tctgctccgg gctaagcact gggctggtga caatttgctg gcaacgttgt tgcagtgcat
     2401 tttcatgaga agtgggcatc ttcttttcct tttatgccga aggtgatgcg ccattgtaag
     2461 aagtttcgtg atgttcactt tgatcctgat gcgtttgcca ccactgacgc attcatttga
     2521 aagtgaatta tttgaaccag atcgcattac agtgatgcaa acttgtaagt agatttcctt
     2581 aattgtgatg tatatcgaag tgtgttgcgg agtagatgtt agaatactaa caaactcgca
     2641 aggtgaattt tattggcgac aagccaggag aatgaaatga ctgatctgaa agcaagcagc
     2701 ctgcgtgcac tgaaattaat ggacctgacc accctgaatg acgacgacac cgacgagaaa
     2761 gtaattgctc tgtgtcatca ggccaaaacc ccggtcggca ataccgccgc tatctgtatc
     2821 tatcctcgct ttatcccgat tgctcgcaaa acgctgaaag agcagggcac cccggaaatc
     2881 cgaatcgcta cggtaaccaa cttcccacac ggtaacgatg acatcgaaat cgcgctggca
     2941 gaaacccgtg cggcaatcgc ctacggtgca gatgaagttg acgttgtgtt cccgtaccgc
     3001 gcgctgatgg cgggcaacga gcaggttggt tttgacctgg tgaaagcctg taaagaggct
     3061 tgcgcggcag cgaatgtact gctgaaagtg atcatcgaaa ccggcgaact gaaagacgaa
     3121 gcgctgatcc gtaaagcgtc tgaaatctcc atcaaagcgg gtgcggactt catcaaaacc
     3181 tctaccggta aagtggctgt gaacgcgacg ccggaaagcg cgcgcatcat gatggaagtg
     3241 atccgtgata tgggcgtaga aaaaaccgtt ggtttcaaac cggcgggcgg cgtgcgtact
     3301 gcggaagatg cgcagaaata tctcgccatc gcagatgaac tgtttggtgc tgactgggct
     3361 gatgcgcgtc actaccgctt tggtgcttcc agcctgctgg caagcctgct gaaagcgctg
     3421 ggtcacggcg acggtaagag cgccagcagc tactaagtaa gatgctttac gcctgatgcg
     3481 ctgcgcttat caggcctacg agacgtatct acccgtaggc cggataaggc gtagacgcat
     3541 ccggcaaaag ccgcctcata ctcttttcct cgggaggtta ccttgtttct cgcacaagaa
     3601 attattcgta aaaaacgtga tggtcatgcg ctgagcgatg aagaaattcg tttctttatc
     3661 aacggtattc gcgacaacac tatctccgaa gggcagattg ccgccctcgc gatgaccatt
     3721 ttcttccacg atatgacaat gcctgagcgt gtctcgctga ccatggcgat gcgagattca
     3781 ggaaccgttc tcgactggaa aagcctgcat ctgaatggcc cgattgttga taaacactcg
     3841 accggcggcg tcggcgatgt gacttcgctg atgttggggc cgatggtcgc agcctgcggc
     3901 ggctatattc cgatgatctc tggtcgcggc ctcggtcata ctggcggtac gctcgacaaa
     3961 ctggaatcca tccctggctt cgacattttc ccggatgaca accgtttccg cgaaattatt
     4021 aaagacgtcg gcgtggcgat tatcggtcag accagctcac tggctccggc ggataaacgt
     4081 ttctacgcga cccgtgatat taccgcaacc gtggactcta tcccgctgat cactgcctcg
     4141 atcctggcga agaaactggc ggaaggtctg gatgcgctgg tgatggacgt gaaagtgggt
     4201 agcggcgcgt ttatgccgac ctacgaactc tctgaagccc ttgccgaagc gattgttggc
     4261 gtggctaacg gcgctggcgt gcgcaccacc gcgctgctca ccgacatgaa tcaggtactg
     4321 gcctccagtg caggtaacgc ggttgaagtt cgtgaagcgg tgcagttcct gacgggtgag
     4381 tatcgtaacc cgcgtctgtt tgatgtcacg atggcgctgt gcgtggagat gctgatctcc
     4441 ggcaaactgg cgaaagatga cgccgaagcg cgcgcgaaat tgcaggcggt gctggacaac
     4501 ggtaaagcgg cagaagtctt tggtcgtatg gtagcggcac aaaaaggccc gaccgacttc
     4561 gttgagaact acgcgaagta tctgccgaca gcgatgctga cgaaagcagt ctatgctgat
     4621 accgaaggtt ttgtcagtga aatggatacc cgcgcgctgg ggatggcagt ggttgcaatg
     4681 ggcggtggac gccgtcaggc atctgacacc atcgattaca gcgtcggctt tactgatatg
     4741 gcgcgtctgg gcgaccaggt agacggtcag cgtccgctgg cggttatcca cgcgaaagac
     4801 gaaaacagct ggcaggaagc ggcgaaagcg gtgaaagcgg caattaaact tgccgataaa
     4861 gcaccggaaa gcacaccaac tgtctatcgt cgtatcagtg aataacggta tactgatctg
     4921 atcatttaaa tttgaagcac tgagtacgga gaacatatga aacgtgcatt tattatggtg
     4981 ctggactcat tcggcatcgg cgctacagaa gatgcagaac gctttggtga cgtcggggct
     5041 gacaccctgg gtcatatcgc agaagcctgt gccaaaggcg aagctgataa cggtcgtaaa
     5101 ggcccgctca atctgccaaa tctgacccgt ctggggctgg cgaaagcaca cgaaggttct
     5161 accggtttca ttccggcggg aatggacggc aacgctgaag ttatcggcgc gtacgcatgg
     5221 gcgcacgaaa tgtcatccgg taaagatacc ccgtctggtc actgggaaat cgccggtgtc
     5281 ccggttctgt ttgagtgggg atatttctct gatcacgaaa acagcttccc gcaagagctg
     5341 ctggataaac tggtcgaacg cgctaatctg ccgggttacc tcggtaactg ccactcttcc
     5401 ggtacggtca ttctggatca gctgggcgaa gagcacatga aaaccggcaa gccgattttc
     5461 tatacctccg ctgactctgt gttccagatt gcctgccacg aagaaacctt tggcctggat
     5521 aaactctacg aactgtgcga aatcgcccgt gaagagctga ccaacggcgg ctacaacatc
     5581 ggtcgtgtta tcgctcgtcc gtttatcggt gacaaagccg gtaacttcca gcgtaccggt
     5641 aaccgtcacg acctggctgt tgagccgcca gcaccgaccg tgctgcagaa actggttgat
     5701 gaaaaacacg gtcaggtggt ttctgtcggt aaaattgccg acatctacgc caactgcggt
     5761 atcaccaaga aagtgaaagc aactggcctg gacgcgctgt ttgacgccac catcaaagag
     5821 atgaaagaag cgggtgataa cactatcgtc ttcaccaact tcgttgactt cgactcttct
     5881 tggggccacc gtcgcgacgt cgccggttat gctgcgggtc tggagctgtt cgaccgccgt
     5941 ctgccggagc tgatgtctct gctgcgcgat gacgacatcc tgatcctcac tgctgaccac
     6001 ggttgtgatc cgacctggac cggtactgac cacacgcgtg aacacattcc ggtactggtt
     6061 tacggcccga aagtaaaacc gggctcactg ggtcaccgtg aaaccttcgc ggatatcggc
     6121 cagactctgg caaaatattt tggtacttct gatatggaat atggcaaagc catgttctga
     6181 taatgggtgc ggcttgatgc cctcaccccg gccctctccc acagggagag ggtgaaaaca
     6241 tgtagataag gataaaacaa tggctacccc acacattaat gcagaaatgg gcgatttcgc
     6301 tgacgtagtt ttgatgccag gcgacccgct gcgtgcaaag tatattgctg aaactttcct
     6361 tgaagatgcc cgtgaagtga acaacgttcg cggtatgctg ggcttcaccg gtacttacaa
     6421 aggccgcaaa atttccgtaa tgggtcacgg tatgggtatc ccgtcctgct ccatctacac
     6481 caaagaactg atcaccgatt tcggcgtgaa gaaaattatc cgcgtgggtt cctgtggcgc
     6541 agttctgccg cacgtaaaac tgcgcgacgt cgttatcggt atgggtgcct gcaccgattc
     6601 caaagttaac cgcatccgtt ttaaagatca tgactttgcc gctatcgctg actttgacat
     6661 ggtgcgtaac gcggtagacg cggctaaagc actgggcgtt gatgctcgcg tgggtaacct
     6721 gttctccgct gacctgttct actctccgga cggcgaaatg ttcgacgtga tggaaaaata
     6781 cggcatcctc ggcgtggaaa tggaagcggc tggtatctac ggcgtggctg cagagtttgg
     6841 cgcgaaagcc ctgaccatct gcaccgtgtc tgaccacatc cgcactcacg agcagaccac
     6901 tgccgctgag cgtcagacca ccttcaacga catgatcaaa atcgcactgg aatccgttct
     6961 gctgggcgat aaagagtaat tgtgtttcgc tgaaaggcga acgtcgtgtt aagccggagc
     7021 aggagactgc tccggctttt tagtttctat t
//
LOCUS       DAZGLJ010000232         7046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000232 DAZGLJ010000000
VERSION     DAZGLJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.232"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            58..354
                     /locus_tag="ACHK49_004181"
     CDS             58..354
                     /locus_tag="ACHK49_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9264716.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            662..778
                     /locus_tag="ACHK49_004182"
     CDS             662..778
                     /locus_tag="ACHK49_004182"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264717.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            997..2430
                     /locus_tag="ACHK49_004183"
     CDS             997..2430
                     /locus_tag="ACHK49_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO9264718.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEERINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNSALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
     gene            3251..3814
                     /locus_tag="ACHK49_004184"
     CDS             3251..3814
                     /locus_tag="ACHK49_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO9264719.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRHN"
     gene            3969..6329
                     /locus_tag="ACHK49_004185"
     CDS             3969..6329
                     /locus_tag="ACHK49_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO9264720.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLIRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIIADGDESVI"
     gene            complement(6491..6667)
                     /locus_tag="ACHK49_004186"
     CDS             complement(6491..6667)
                     /locus_tag="ACHK49_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264721.1"
                     /translation="MTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMA
                     NYQLDEQLLEGCRP"
     gene            complement(6805..7046)
                     /locus_tag="ACHK49_004187"
                     /pseudo
     CDS             complement(6805..7046)
                     /locus_tag="ACHK49_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         1949 a         1369 c         1714 g         2014 t
ORIGIN      
        1 aaatttaccc ggggaataca aactgtaacg acagttcacc acactatggg ggataaaatg
       61 ccagatcaat ccggacactg caggactcct tcacgcatct tacggatgcc tgaactgagc
      121 caattgttag gtatatctcg ttcgacaatt tacgaaaaaa tgaatccgtt atcgaaatac
      181 tacgatgcaa ccttccccag gccggtacgt cttggtagtg gttctgtggg ctggcgttca
      241 tcagctattg acgaatggct tactttacat actgtacctg ccagatcagc agtcaaaggt
      301 gttaatgacg aagtgagaca tacgctttct tcagatatga ccatgagcag ataagttttt
      361 gtcatcacgt gatcacctca ggaggaaaaa gaagcataag aacaatttag ccagaagttt
      421 aattgataaa cagaatttcc agcattcatt gttgaaagag cagctattgt cgctattgta
      481 gcggtaattg ctgctataac cgaaacgtat tacgaaaaag gaagcattta tgccttacat
      541 caaaccggat atgtcgtatt cagtatatgc atgaccatca atgataggga aggcaataat
      601 taaagctcaa tacaataaac aagtacctct gactctggtt gcaatgtcag tattaacagc
      661 gatgtcaatt gcctgtcaga atcaggttga cgtatgcagt cccggaaatt tacgtggggc
      721 cgttaatatt tacactatgg ttctggagaa agggaaaaga ccgggggaaa ggtattagtg
      781 aaataatttc cggaggtatt aagaatgaga aatatgaatg atatttcgtg gtgaataata
      841 cagtcttcac tgaattaacc ttatcggttt attaaacatc agatctacaa atgctctgta
      901 tttctcagag catcaggtct gaatagcagc tattttcgct attgtagcgg gatttgctgc
      961 tattttacta acccattaat gaaaagagag gcttttatgt gcttacttgc accagaaaat
     1021 ccttatccga tatatgcact accaccgctg gtgagaaatg caataattga aactcaaaaa
     1081 aatacacagg cacctttggc tatggtggca acatccgcat taacagcgat ctcaattgcc
     1141 tgtcagaatc agattgacgt gtgcagacct ggaaatttac atgggcctgt taatctttac
     1201 tctctgattc tggctgattc cggtgaaagg aaaacgaccg tggataaagt gtttatgaaa
     1261 gcattttatc tcagggatga agccctggcg gatgaatacg cgaaactggt tgagaattac
     1321 agtacagaaa aggaaatatg ggagcaaaaa caaaaagcgc tggaatcaaa atttcataaa
     1381 gagattcgtg ccggtaaaga ttacaaggca acagaatcag agcttgaaac gcatctgaat
     1441 aaacctcctg ttccgccgca gatacgtcga acaattttta atgagacaac gatagaggga
     1501 atgttaaaat attactccga tagcaatcgc tcttttgctc ttgtatccag tgaagggggg
     1561 gtaatttttg acagccgggc catgagtaaa ctgggaatta ttaacacttt gtgggatgga
     1621 ggttctcttt tcatcgacag gaaatcatct cccggaatta atttgaagga gccaagactg
     1681 acgatatcgg tgatgattca gcctgatgtt taccacaaag gtttttgtac gcgaaaaaaa
     1741 gaaattgtga aaacgtcagg acatcatgca aggtttttga tgtgtcaacc aacatcaacg
     1801 caggggacaa ggataataac cggagataat tattcatcac agtatcagga tttgtttgaa
     1861 gagaggatca atgagcttat tgatgaaagt ctggcgatga gtggtgaacg acgttgcctt
     1921 cacttttctc ctcaggctgc cagaatctgg acggattatt acaatgatgt ggaatcgaag
     1981 ctggggggat tggggccttt aagacattgc cgggaatatg ctgctaaaaa tgcagagtat
     2041 atggcaagac tggcaggact tatttaccat tcgagcggtg aagaggggga gatttcccct
     2101 tacactgcag aaatggcgag agaattagca atatggtacg gtaatgagta tgtgcggttg
     2161 tctaatccgt taacttttga caactctgcc ctgaccgtac ctgtgcgact cattccagag
     2221 gagcttgaac ttttcaactg gataaaaagc tattgtattg agaaggggat cctctgtatg
     2281 aaaaaaaatg acattttaca gcgtggtccg aaccgtttcc ggaaaaagga taaaatcaac
     2341 tggttactgg atttactgta tgaacaaaac agagttgtac cggttattga gggaaaaacg
     2401 ttgtgtgtcg cacctaactt tgacctctga acgacttaat cgttgagtac atatagtctc
     2461 ctctgctact tgcggaggag actaatgtct ttaccatgtc agcgttgtat tagaaaactc
     2521 tggtacaggt cagtcagtaa atataactgg tgtaaccact atttactatt ggatagagtg
     2581 ttactcagga tattgtcggc tttagtctga ggtgaggtgg ggccttcatt gttttcgtgt
     2641 tcaataaata atttaggtta attagtatta ttagtggaag ttatgtgatc tgttatgttg
     2701 tcttgtacgc acttaacact ggttacgtac cggaacacta ttagagtatg ttatggctct
     2761 gttacagttt ataggtatga aaaaatataa tctttcctgt atgaaataat attcttatgg
     2821 tgtgtggggt atggtctgta taccatcatt tttatggtga agttaaatat ctgtagaggc
     2881 ctcaagctgg gtcgctgttt ttacttaatg gtacagaagt attaatagcg gtgttgcctg
     2941 tggctacccg atactggctt ttgtgaatgt cagattactt cctgtaagtg attgaatggt
     3001 acttccttaa actcaattct atgaggtgtg tttttaccat tgtaattcgt actggtcctc
     3061 agtttaagtg agtcttactg tactctgaaa tattacctct ttggagtaat gaggtaatac
     3121 gaaacagttt tatcaggttg tctgtgctgc tggttacctc gtttattaat gccgcggtac
     3181 ggcgtttctg tactctcagt atttatgtaa ttccaccttc ctgtaacttt tataaagtaa
     3241 ggactataaa atgtataatg ctaatcccaa ttacgaaatg gacttcatga ttctgaaaga
     3301 cgtgaatgaa cacatggaag gtctgtttca acgtttttct aaactgttac ctttcagaat
     3361 tgattttgcc tacaggaaag acacgcccag ttttggtcat tcctgtaggc acagtatgtg
     3421 tattgaaatg caccgcttac ttagtgaaac ccaaacgatg ctggcgggat attactgggt
     3481 aatggaatat acgtcaaaca agggacttca tattcatttt atcggctacc tggatgggca
     3541 acgtcacaac aaatcttatc ggatatccag gcaactggga gatatctgga ggcggatcac
     3601 ggaaggggag ggttattttc acctgtgcag ggctaaagac aaatacccgg tgcgtatcga
     3661 tcacgtaatt cactatagtg ataaatctgc ggtagacgac ttacgttacg cccttagcta
     3721 tctggccaaa caggatcaga aagaacacgg tattattctg aggcgtagca ggcttcccga
     3781 aaaaagtaac cggggccgac cacgacataa ctgaattaca gctgtattag gtattagaat
     3841 taagtttctg taagttgttg ggaggtgctt gagttaatag gcagaagagg gcggggcgct
     3901 ttgtcatcag tgtaggggac ccgaatttct gccgtggtgc ctcagtggat cggatacagg
     3961 taacgaatat ggttcataaa tctgacagtg atgaattagc tgcattgagg gcagaaaatg
     4021 cccgtctggt ctcattactt gaagctcatg ggattgaatg gcgacgtaaa ccgcagactc
     4081 ctgtgcagcg cgtttctgta ttatccaccg acgagaaggt tgcattattt cgtcggttgt
     4141 ttcgtgggcg tgatgatgta tgggcactta gatgggaaag taaaaccagc ggcaaatcag
     4201 ggtattctcc tgcctgcgct aacgaatggc aggcgagaat atgcggtaaa ccccggataa
     4261 aatgtgggga ctgcgctcac cgtcagttga ttcctgtatc cgatctcgtc atctaccacc
     4321 atctggccgg tacccatact gtcgggatgt atccgttgct ggaagatgat tcctgttatt
     4381 ttctggcagt tgattttgat gaagctgaat ggcagaagga tgcatccgca ttcatgcgat
     4441 cctgtgatga gctgggtgta cctgctgcgc tggaaatatc ccgttcacgt cagggggcgc
     4501 atgtctggat attttttgcc tcacgagttt cggcccgcga agctcgccgt ctggggactg
     4561 ctattattag ctatacgtgt agtcggaccc gacagctgcg attggggtct tatgaccgat
     4621 tatttcctaa tcaggatact atgccaaaag ggggatttgg taatctcata gcgttacctc
     4681 tgcaaaaaag accgcgtgaa ttggggggaa gcgtttttgt tgatatgaat ctccagcctt
     4741 atcctgatca gtgggctttt cttgtatcgg tgaccccgat gaatgtgcag gatattgaac
     4801 cgacgatatt acgggctaca gggagtatcc atcctctgga tgtgaatttt atcaacgaag
     4861 aagacctggg tacgccgtgg gaagagaaaa aatcatcagg aaacagactg aatatttcta
     4921 ttgcagaacc gctgaaaatc acgctggcaa accagatcta tttcgaaaaa gcgcaattac
     4981 ctcaggtgct gattaaccga cttattcggc tggcagcatt tccgaaccct gagttttata
     5041 aggctcaggc aatgcgtatg tcagtctgga ataagccccg tgttataggt tgtgcggaga
     5101 attacccgca acacattgcg ttgccccggg gatgtctgga cagcgtatta tctttcctta
     5161 gggacaacaa tattgctgca gaattaatcg ataaacgatt tgccgggacg gaatgtaatg
     5221 ccgtttttat gggaaacctc agagcggagc aggaagaggc cgtttcggca ttactccgtt
     5281 atgacactgg tgtgctttgt gcgccaacgg cttttggtaa gacagttacc gcagcggcag
     5341 tgattgccaa gcggaaagtg aatacactga tactggtaca ccggactgaa ttgctgaagc
     5401 agtggcagga gcgtctcgcg gtgtttctgc aggccggtga cagtattggt attatcgggg
     5461 gaggtaaaca taaaccctgt ggcaatattg atattgcggt ggtgcagtcc atatccagac
     5521 aaggagaagt tgaacctctg gtcaggaatt atgggcaaat cattgtggat gagtgccatc
     5581 atattggcgc ggtttcattt tctgcgattc tgaaggaaac gaatgccaga tatctgcttg
     5641 gcctgacggc aacaccaatc cgacgggatg gtctgcatcc cattattttt atgtactgtg
     5701 gtgccattcg ccatacagcg gtccgcccga aggaaagccc acataatctg gaggtactga
     5761 tccgttcccg ttttacatct ggtcatttac catcggatgc gagaatccag gatattttca
     5821 gagaaattgc tctggatcat gacagaacgg tggcgatagc tgaagaagcc atgaaagctt
     5881 tcgggcaggg gcgaaaagtt ctggtactga ctgaacgtac agatcatctg gatgagatag
     5941 catcagtgat gaattcactg aaattgtctc cctttattct ccatggtcga ctatcgaaaa
     6001 aaaagcgtgc gatgctgata tccgggctga atgctcttcc tcccgattct cctcgaattt
     6061 tgttgtcaac aggcagactt attggtgagg gatttgacca ccctccgctg gatacgctga
     6121 ttcttgccat gcctgtgtca tggaaaggga cattacagca gtatgcaggg cgtcttcaca
     6181 gagagcatac cggtaaaagc gatgtcagga tcattgattt tgtggatacc gcgtatcctg
     6241 tgctgctcag aatgtgggat aaacgtcagc ggggttataa agcgatgggg tacaggatta
     6301 tagctgacgg cgatgaatca gtgatttaat tttaaggagt actttaccct ttttaaataa
     6361 tttgagctcc ccgggttgtt cgggcaacaa agagtcccag tatacaggct tcaggaatat
     6421 gttgtagtat caggcttttc atcgctttaa aatgactacc ggcagtgaga acgtcatcaa
     6481 aaataacaat ctagggcctg cacccttcca gtaattgttc atccagttgg tagtttgcca
     6541 taatcgcttc tggtaagggc cggtttgttg cggtatgaga ggctattgtt gatacgtttt
     6601 gcacaaccag ttcccagaaa tccagccatg gttcgtaggt ttgcgcaatt cgcagaattt
     6661 ctgtcatacg gtcatcgtaa gcggcatctg tacggcattt ggatggtggt acgggaacaa
     6721 atgtgtattc ctcaagatta ccgatatttg atatgagcat ctgagcaaca tactcaatag
     6781 cagccttttt atagtggtaa tcaggctacg aaagtacagc gttttataaa ttgtctcagg
     6841 tgatattcgc agcgtttttt gacgtggttt tgttcgcctt aaccatcctg atatttgctc
     6901 tggagaccat ttcatctcca gcttttccag aacaagcttt cgcaatggta aattttgatc
     6961 cagtaagcac ggttttggcc ttttcgccat tctgttggct cggttattag catcaacagc
     7021 tttgtaatag cgtctgcccc gattac
//
LOCUS       DAZGLJ010000233         7033 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000233 DAZGLJ010000000
VERSION     DAZGLJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.233"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(90..1156)
                     /locus_tag="ACHK49_004188"
                     /pseudo
     CDS             complement(90..1156)
                     /locus_tag="ACHK49_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(1159..1521)
                     /locus_tag="ACHK49_004189"
     CDS             complement(1159..1521)
                     /locus_tag="ACHK49_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO9264722.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2058..3971
                     /gene="mtlA"
                     /locus_tag="ACHK49_004190"
     CDS             2058..3971
                     /gene="mtlA"
                     /locus_tag="ACHK49_004190"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO9264723.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSTILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGEYIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4201..5349
                     /gene="mtlD"
                     /locus_tag="ACHK49_004191"
     CDS             4201..5349
                     /gene="mtlD"
                     /locus_tag="ACHK49_004191"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO9264724.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5349..5936
                     /gene="mtlR"
                     /locus_tag="ACHK49_004192"
     CDS             5349..5936
                     /gene="mtlR"
                     /locus_tag="ACHK49_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO9264725.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(5948..6157)
                     /gene="yibT"
                     /locus_tag="ACHK49_004193"
     CDS             complement(5948..6157)
                     /gene="yibT"
                     /locus_tag="ACHK49_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO9264726.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6442..6804
                     /locus_tag="ACHK49_004194"
     CDS             6442..6804
                     /locus_tag="ACHK49_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO9264727.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1779 a         1765 c         1792 g         1697 t
ORIGIN      
        1 taaagaaaaa tcatcgcagc taatgaagta atatgcaaaa ataattagtc cttacaagga
       61 gcaaagttcc ataatctccc ccctccccct caatgatcca aataaagata atgcatccag
      121 ctggtcattc ttagcagcac tttccgcatc accgaaacat ggctgaaatg gtcggagtaa
      181 accgccacct gggcgtagat gccgtcgggt aaggcatcga tatcatcgtt agggtccagt
      241 tcaatggttc ccagcacacc gtccgtgccg ggcacgaccg ttaatgattg caataccccc
      301 tgcgcctgat aagaaccgcc tggcacgaca ggtaaaatac tggtcagttt gccgtggaac
      361 acctgcccag gtagcgcgtt aaacaccact tctgcatcat caccaggttt cagacgtaac
      421 agcgagtttt gccgaaattg ggcgacaatt tgccgttttt gctcggggat gaaaaccatc
      481 accggacgca acggcaaggc agctgcgtat gtacctgggc ggatcagtac ctgagtaatg
      541 tagccattgc tcggcgcgcg aatgacagtc tgctcaagat tatattttgc ttcagtaagt
      601 tgcgctctta agctcacaat ctgcgattgc tcgccgttaa ccatactgtc gagctggctc
      661 tggatctgcg cctgctccgc caccgagcct ttcaccagcg catcctgcgc gaggaaattt
      721 tgccgcgcat cgtcgatgtc acgttccgag aacggattca ccgccgcctg gctgcctttc
      781 agataacgtt gataattttt aaacagacgg tcgcgctccg ctgaaacctg ggtggtgttg
      841 gcctgcgctt ctgtgagctg cgcgcgcaga gtctttatat tatgcgtcgc cgtcatcagg
      901 tcagcctgaa gcctgtcaac tcgcgcctgg taacgaaccg ggtcgagctt aaaaagcacc
      961 tcgccctttt gaataagctg attattcttg tcagtgactt cagtaacaat tcccgtcacc
     1021 tgtggcgtga tggggatcgc tatcactgcc ttttgcgcgg taaaagtgta agggtggttg
     1081 tagttcatca acaaaatcaa accactcacc agaaacacgc cagcgccacg taagttaaaa
     1141 caatcaatag atccataatt agatctccgg gaacgtggaa tagtcagtct ttttctcggc
     1201 ggcgagttga tgctccagcc gggaaattcg gtcagaaagt gcggcgattt ccgggtcagt
     1261 cgctttctcc tgcgacgcaa catgtgactg cataccccag ccacgctccg gttgatagag
     1321 cgtcgcccag atccacagaa acggccagat aacatgcaga gtaaacaggc tcacccagcc
     1381 cgccgtatga atagcgtcgg catggggatg gttgcgcttt ttggcaatca gataagggat
     1441 gtcatgtatc gcgatgattc cataaaaaat caccagaaag acgaagatca gcactcccaa
     1501 cgcgaaatag tttagaaaca tatctgcctc ggattcacgt ttatcagtgt tgtttttggg
     1561 ctggcagcca gaagggagtc aggctgatat tttgacaata atccgggttc gcgattctcg
     1621 ccataacacc aaagaataat ttttagaggt gatgagttgc ttagttacat aacgattgta
     1681 tgacgaaggc ataacatgct gtagatcaca tcaggtgaac gccgtaagaa aatatcttgt
     1741 gattcagatc acaaagattc aacaaaccat caaaacaaaa atgtgacact actcacattt
     1801 aaatgccatt tttagcgaaa atcgccgcct tgttgctttt ttacacaagc gttttgtgat
     1861 gaacgtcacg tcaattacct ctctaccccc tatatttatg tgattgatat cacacaaaag
     1921 gccgtcgact ggacagttaa ccgattcagt gccagatttc gcagtatcta caaggtccgg
     1981 ctacctctgc cgccacatta acaaaaaacc tcgggcttcc agcctgcgcg acagcaaaca
     2041 taagaagggg tgtttttatg tcatccgata ttaagatcaa agtgcaaagc tttggtcgtt
     2101 tcctcagcaa catggtgatg ccaaatatcg gcgcgtttat cgcgtggggt atcatcaccg
     2161 cgttatttat tccaacaggg tggttaccga acgagacgct ggcgaagctg gtcgggccga
     2221 tgatcactta tctcctgccg ctgctgatcg gttataccgg tggtaagctg gtaggcggcg
     2281 aacgtggcgg cgtagtcggt gccatcacca ccatgggcgt tatcgtcggc gcagacatgc
     2341 cgatgttcct cggttctatg attgcaggtc cgctgggcgg ctggtgcatt aagcacttcg
     2401 accgctgggt agacggtaag atcaaatccg gttttgagat gctggtgaat aacttctccg
     2461 caggcatcat cgggatgatc ctcgctattc tggcattcct cggcattggc ccgattgttg
     2521 aagccctgtc caaaatgctg gctgcgggcg ttaacttcat ggttgtccat gacatgctgc
     2581 cgctggcgtc tatctttgtt gaaccggcga aaatcctgtt cctcaacaat gccattaacc
     2641 acggtatctt ctcgccgctg ggtattcagc agtcccatga actgggtaaa tcaatcttct
     2701 tcctgattga agctaaccca ggtccaggta tgggcgtgct gctggcgtac atgttctttg
     2761 gtcgtggtag cgctaaacag tctgcgggcg gtgcggcaat catccacttc ctgggtggta
     2821 tccacgaaat ctacttcccg tatgtgctga tgaatccgcg tctgatcctc gcagtcatcc
     2881 tcggcggtat gactggcgtg ttcacgctga ctatcctggg cggtggtctg gtttctccgg
     2941 catctccggg ttctatcctt gctgtactgg cgatgacacc aaaaggtgct tatttcgcta
     3001 acatcgcggg tgtgtgtgcg gcgatggctg tctccttcgt tgtctctact attttgctga
     3061 aaaccagcaa agtgaaagaa gaagatgata ttgaagcagc aactcgtcgt atgcaggaca
     3121 tgaaagctga gtctaaaggc acatctccgc tgtctgcggg cgatgtgact aacgacctga
     3181 gccacgtacg taaaatcatc gttgcctgtg acgccggtat gggttcaagt gcgatgggcg
     3241 caggcgtgct gcgtaagaaa attcaggatg caggtctgtc acagatttcg gtcactaaca
     3301 gcgcgatcaa caacctgccg ccagatgtgg acctcgtcat cactcaccgt gacctgaccg
     3361 aacgcgctat gcgccaggtt ccgcaggcgc agcatatttc gctgaccaac ttcctcgaca
     3421 gcggcctgta caccagccta accgaacgtc tcgttgctgc ccagcgccat actgaaaacg
     3481 aagtgaaagt gaaagacagt ctgaaagaca gctttgacga ttccagtgct aacctgttca
     3541 aactgggcgc ggagaacatc ttcctcggtc gcaaagcagc aaccaaagaa gaagcgattc
     3601 gttttgctgg cgagcagctg gtgaaaggcg gttacgttga gccggaatac gttcaggcga
     3661 tgctggatcg tgaaaaactg accccgactt atctgggtga gtatatcgcg gtgccacacg
     3721 gtacggttga agcgaaagat cgcgtactga aaacgggcgt ggtgttctgc cagtacccgg
     3781 aaggcgtgcg cttcggtgaa gaagaagatg acattgcccg tctggtgatt ggtattgcag
     3841 cccgtaacaa cgagcacatt caggttatca ccagcctgac caatgcactg gatgatgagt
     3901 ccgtcatcga gcgtctggca cacaccacca gcgtggatga agtgctggaa ctgctggcag
     3961 gtcgtaagta atccaatccc accctctcca catggagaag gtggggttaa ttgcctgatg
     4021 cgctacgctt atcaggccta caggatgcat cacaatttgt tgaatttgca cgttcttgta
     4081 ggccggataa ggcgcttacg ccgcatccgg cgctgcccct ctcctcacgg agagggtttg
     4141 ggtgagggaa aagcctcacc ccagccctct cgggtaaaaa cattgatgaa ggttaatact
     4201 atgaaagcat tacattttgg cgcaggtaat atcggtcgtg gctttatcgg taaactgctg
     4261 gcagacgcgg gtatccaact gacgtttgcc gatgtcaatc aggtggtact tgatgccctg
     4321 aatgcccgtc atagctatca ggttcatgtg gttggtgaaa ccgagcaggt agataccgtt
     4381 tccggcgtca atgctgtcag cagcattggt gatgatgtcg ttgatctgat tgctcaggtc
     4441 gatttagtca ctaccgccgt tggcccggtt gtgctggaac gtattgctcc ggctatcgcc
     4501 aaagggctgg tgaaacgcaa agaacaaggt aatgaatccc cgctgaacat catcgcctgt
     4561 gaaaacatgg tacgcggtac cacgcagctg aaaggccatg tgatgaacgc cctgccggaa
     4621 gacgccaaag cgtgggtaga agaacacgtt ggctttgtcg attccgccgt tgaccgcatc
     4681 gtaccgcctt cggcttcggc aactaacgat ccgctggaag tgacggtaga aactttcagc
     4741 gaatggattg tcgataaaac gcagttcaaa ggcgcactgc cgaacatccc aggcatggag
     4801 ttaaccgaca acctgatggc atttgtcgaa cgtaaactct tcaccctgaa cacgggtcat
     4861 gctataaccg cgtacctcgg aaaactggcc ggtcatcaga ccattcgtga cgctattctc
     4921 gacgagaaaa tccgcgcggt ggtaaaaggt gcgatggaag aaagtggtgc ggtactgatc
     4981 aagcgctacg gctttgacgc agacaaacat gcggcgtaca tccagaaaat cctcggtcgt
     5041 tttgagaacc cgtatctgaa agatgatgta gagcgcgtag gccgtcagcc gctgcgtaaa
     5101 ctgagtgctg gcgaccgtct gatcaagcca ctgctcggta cgctggaata cagccttccg
     5161 cacaagaatc tgattcaggg gattgctggt gcaatgcact tccgcagtga agatgatccg
     5221 caggctcagg aactggcagc actgatcgct gacaaaggtc cgcaggcggc gctggcacag
     5281 atttccggtc ttgatgccaa cagcgaggtt gtatccgagg cggtaaccgc ttataaagca
     5341 atgcaataat ggtggaccag gcgcaggaca ccctgcgccc gaataacaga ttgtcagata
     5401 tgcaggcaac aatggaacaa acccaggcct ttgaaaaccg tgtgcttgag cgtctgaatg
     5461 ctggcaaaac cgtgcgaagc tttctgatca ccgccgtcga gcttttgacc gaggcggtaa
     5521 atcttctggt gcttcaggta ttccgcaaag acgattacgc ggtgaagtat gctgtagaac
     5581 cgttactcga cggcgacggt ccgctgggcg atctttctgt gcgtttaaaa ctcatttacg
     5641 ggttgggcgt cattaaccgc caggaatacg aagatgcgga gctgctgatg gcattgcgtg
     5701 aagaactaaa tcacgacggc aacgagtacg cctttaccga cgacgagatc cttggaccct
     5761 ttggtgaact gcactgcgtg gcggcgttac caccgccgcc acagtttgaa ccagcagact
     5821 ccagtttgta tgcaatgcaa attcagcgct atcaacaggc tgtgcgatca acaatggtcc
     5881 tttcactgac tgagctgatt tccagaatca gcttaaaaaa agcctttcaa aagtaagcaa
     5941 cgtctgctta ctgcccctct acctgcttcg gccgataaag ccgacgataa tactccagac
     6001 gttgtagata taacggcacg ctttcatcgg gtattccgga cggaatcgcg ttacggggag
     6061 gaagtttttt cagatactcc cggaacgcct ggcttgatgc catgaaatct acggctttat
     6121 cgataagaag cggaacgttt tcacctaatt tgcccatgat gatatctccg tattaccccg
     6181 cgccgggaat gcgcggccgc caattttagt ttaggttccc ggtaaagatg acattaagga
     6241 aaacgtgctg aatcctcaat aatcatccgc tgctgtttat tatctttttt ctatagattt
     6301 cttaattaat caacgaattg cgtagttttt gagcatgtta atgattacgt tgcagaacta
     6361 ttacaatgtc atcagttgtg aacaaagcac ctggtcgcgc atactagggg ctataaattt
     6421 atctttatca gaagccatca catgaaagaa gtcgaaaaaa acgaaatcaa acgtctcagc
     6481 gatcgcctgg acgccatccg ccaccagcag gccgatctgt cgctggttga agccgcagac
     6541 aaatatgccg agctggaaaa agagaaagcc acgctggaag cagaaattgc tcgcctgcgt
     6601 gaggttcata gccaaaaact gagtaaagaa gcacagaagc tgatgaagat gccgtttcag
     6661 cgcgcaatta ccaaaaaaga gcaggctgat atgggcaagc tgaagaaaag tgttcgcgga
     6721 ctggtggttg tgcacccaat gaccgcgctg ggccgtgaaa tgggcctgca ggagatgact
     6781 ggattctcta agactgcgtt ttaagcgcct cagacgactg gttcgatcat atcaacccgc
     6841 atctggcttg ccttagctgg tcaatgcggg gactctcctg tctactcgcc taattaccaa
     6901 acaattcact ccctttacgt cgatttaccg ctggttgata ttctgaaaat gcatttttac
     6961 cgaaatagcc acctataaac aataaaaata tatagaagat atcatttatt aattaacaag
     7021 catttacgtc tct
//
LOCUS       DAZGLJ010000234         6962 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000234 DAZGLJ010000000
VERSION     DAZGLJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.234"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            178..948
                     /gene="yjjP"
                     /locus_tag="ACHK49_004195"
     CDS             178..948
                     /gene="yjjP"
                     /locus_tag="ACHK49_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO9264728.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSDTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            939..1412
                     /gene="yjjB"
                     /locus_tag="ACHK49_004196"
     CDS             939..1412
                     /gene="yjjB"
                     /locus_tag="ACHK49_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO9264729.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1519..2058
                     /gene="dnaT"
                     /locus_tag="ACHK49_004197"
     CDS             1519..2058
                     /gene="dnaT"
                     /locus_tag="ACHK49_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO9264730.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2061..2798
                     /gene="dnaC"
                     /locus_tag="ACHK49_004198"
     CDS             2061..2798
                     /gene="dnaC"
                     /locus_tag="ACHK49_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO9264731.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2847..3341
                     /gene="yjjA"
                     /locus_tag="ACHK49_004199"
     CDS             2847..3341
                     /gene="yjjA"
                     /locus_tag="ACHK49_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO9264732.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3595..5886
                     /gene="opgB"
                     /locus_tag="ACHK49_004200"
     CDS             3595..5886
                     /gene="opgB"
                     /locus_tag="ACHK49_004200"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO9264733.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGAGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6025..>6962)
                     /gene="lgoD"
                     /locus_tag="ACHK49_004201"
     CDS             complement(6025..>6962)
                     /gene="lgoD"
                     /locus_tag="ACHK49_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFO9264734.1"
                     /translation="ALIKIKTVGICGTDIHAWAGNQPFFSYPRVLGHEICGEIIESGK
                     NFHNFNIGQQVAVIPYISCQQCPACKSGRTNCCEKISVIGVHQDGGFSEYLSVPVANI
                     LPADGIDPQAAALIEPFAISAHAVRRAAIAPGEQVLVVGAGPIGLGAAAIAKADGAQV
                     VVADTSPARREHVATRLELPVLDPSAEDFDAQLRAQFGGSLAQKVIDATGNQHAMNNT
                     VNLIRHGGTVVFVGLFKGELQFSDPEFHKKETTMMGSRNATPEDFAKVGRLMAEGKIT
                     AYMMLTHRYPFATLAETYERDVINNRELIKGVITF"
BASE COUNT         1706 a         1836 c         1756 g         1664 t
ORIGIN      
        1 tgatagtttg tttgcggcga gagataattc gctttttatc accgagcttc tcccgataac
       61 caggattaat cagcgcaatt ttgatcttaa cctaccgcaa aacaaccgtg cttcatgata
      121 caaatggatt ctcattatct gaacaatacg cagcacgtct atgacaaagg gcgagttatg
      181 caaactgagc aacagcgagc cgtaacacgg ctttgtatcc agtgtggatt atttcttttg
      241 caacatggtg cggaaagcgc gttggttgat gagctttcct cacgactggg tcgggcgctg
      301 ggaatggaca gcgtcgaaag ttctatctct tcgaacgcca tagtgctgac aaccattaaa
      361 gatgggcaat gcctgacatc gacacgtaaa aatcacgatc gcggcattaa tatgcatgtg
      421 gtgactgaag tccagcacat tgtgattctt gcggagcatc atctgctgga ttacaaaggc
      481 gtagagaaac gatttagcca aattcagcca ttacgttacc caagatggct ggtagcctta
      541 atggttggcc tttcttgcgc ctgtttctgt aaactcaata acggtggctg ggatggtgcc
      601 gtcatcacct tctttgccag tacgaccgcg atgtatatcc gccagctgct ggcacaacgt
      661 catcttcatc cacagatcaa cttttgcctt accgctttcg ccgccaccac catttccgga
      721 ttgcttttgc aactccccac tttcagcgat acacccacca ttgcgatggc cgccagcgtt
      781 ctgctgctgg tgccgggctt tccgttgatt aatgccgtcg ccgatatgtt taaaggccac
      841 atcaataccg gactggcacg ctgggcgatc gccagtctgc tgacgctggc tacctgcgtc
      901 ggcgtggtga tggcactgac gatttggggg ctacgcggat gggtgtgatc gaatttctgt
      961 tagcgttggc gcaggatatg atcctcgccg ccattcctgc ggtcggcttt gcgatggtgt
     1021 tcaacgttcc cgttcgggcg ttacgctggt gtgcgctgct tggcgcgatt ggtcatggtt
     1081 cacgaatgat cttaatgacc agcgggctga atattgagtg gtcaactttt atggcttcta
     1141 tgctggtcgg caccattggt attcaatggt cgcgctggta tctggcgcat ccgaaagtgt
     1201 ttaccgtggc ggccgttatc cctatgttcc cgggcatatc ggcttatacc gcaatgattt
     1261 cggcggtaaa aatcagccag ttaggttaca gcgaaccgtt gatgattacc ctgttaacca
     1321 actttcttac agcttcatcg attgttggtg cgttatccat cggtctttcc attcctggat
     1381 tatggttgta ccgcaagcgc cctcgcgtat aaaattgcct cgcttaagca cacggatgag
     1441 agacagcctc ctctcctccg tgtgttacta taaaagttat ctcccttctc gttcatcgtt
     1501 ccatatttga gaaacagtat gtcttccaga gttttgaccc cggacgtcgt tggtattgac
     1561 gccctggtac acgatcacca aaccgttctg gcaaaagctg aaggcggtgt ggttgccgta
     1621 tttgctaaca atgccccggc gttttatgcc gtcacgcctg cacgcctggc tgaactgctg
     1681 gcgctggaag aaaagctggc gcgtccggga agcgatgtcg ctctggacga tcaactctat
     1741 caggaaccgc aagccgctcc cgttgctgtc cccatgggga aattcgccat gtatccggac
     1801 tggcaacccg atgccgactt tatccgcctg gcggcgctat ggggcgtggc gctaagagag
     1861 ccggtgacca ccgaagaact ggcctcattc attgcctact ggcaggcgga aggtaaagtc
     1921 tttcaccatg tgcagtggca acaaaaactg gcgcgcagcc tgcaaatcgg tcgtgccagc
     1981 aacggcggac tgccgaaacg agatgtgaat acggtcagcg aacctgacag ccaaattcca
     2041 ccaggattca gagggtaacg atgaaaaacg ttggcgacct gatgcaacgc ctgcaaaaaa
     2101 tgatgcctgc ccatatcaaa cctgcattca aaacgggtga agagctactg gcctggcaga
     2161 aagaacaagg ggcgatccgc tccgccgctc tcgaacgtga aaatcgggcg atgaaaatgc
     2221 agcgcacctt taaccgctcc ggtattcgtc cactgcatca gaactgctcc tttgagaact
     2281 atcgcgttga gtgtgaaggg cagatgaatg cgttaagcaa agcgcgccag tatgtcgaag
     2341 agtttgacgg caacatcgcc agcttcatct tttctggtaa gccaggaacc ggcaaaaacc
     2401 atctggcggc ggcaatctgc aacgagctgc tgctacgcgg taaatccgta ttgatcatca
     2461 ccgtggccga tattatgtcg gcgatgaaag ataccttcag gaatagcggt accagcgaag
     2521 aacaactgct taacgatctg agcaacgttg atctgctggt gatcgatgag atcggcgtgc
     2581 agaccgaatc gaaatacgaa aaagtgatca tcaaccagat cgtcgatcgc cgctcttctt
     2641 ccaaacgccc aaccgggatg ctgaccaaca gtaatatgga agagatgacc aagttgctgg
     2701 gcgaacgcgt gatggaccgt atgcgcctgg gtaacagttt gtgggtgatc ttcaactggg
     2761 atagctaccg tagccgggta acaggtaaag agtattaatc cccgtttctg gcggtatact
     2821 gcgacacaat tccaccttga gtaatgatga tgaaaactgt taaacatctt ctgtgctgtg
     2881 ccattgccgc cagcgcatta atttccaccg gggtgcatgc tgcgtcctgg aaagatgcgc
     2941 tctccagcgc cgccagcgaa cttggcaacc aaaacagcac gacacaggaa ggcggttggt
     3001 cgctcgcgtc attaactaac ttgcttagca gcgggaacca ggccttaagc gcagataaca
     3061 tgaacaacgc cgcaggcatt ctgcaatact gcgcgaagca aaagctggcg tcggtaaccg
     3121 atgccgaaaa catcaagaac caggtgctgg aaaagctggg cctgaacagt gaagaacaaa
     3181 aagaagacac caactatctg gatggcattc agggtttgct gaaaaccaaa gatggtcagc
     3241 aactcaatct ggataacatc ggaacgactc cgctggcaga aaaggtgaaa accaaagcct
     3301 gcgatctggt gttaaagcag gggctgaact tcatttcctg aaccgcgaaa cgccgcgttt
     3361 tagctgttac ccggctgacg cggctctctc ctgccctttt ccctgctcct ggacggtttt
     3421 ccccctgtta cacgggaagc gaccgcgatc aaaaaagtct gtttataaac taattcctaa
     3481 caacatcgca tttttttgac actagaattg cagcaatatg gcgacgccat tacattgtgc
     3541 ttctcaaaaa gcttaaacgt tggctaaacg gcccggttta gtgaggatcg cccgttgtca
     3601 gaactactct ctttcgccct ttttctcgcc tctgtgctga tttacgcatg gaaagcggga
     3661 cgtaacacct ggtggtttgc agccacatta acggtgctgg ggctatttgt cgttttaaat
     3721 atcaccctgt ttgccagcga ctattttact ggcgctggta ttaacgacgc ggttctctat
     3781 accttaacca acagcctgac cggtgctggc gtcagcaaat acatcctgcc gggtatcggc
     3841 attgtgctgg ggctgacagc ggtgttcggt gcgctgggct ggatcctgcg ccgtcgtcgc
     3901 catcatccgc accattttgg ttacagcctg ctggcgctct tactggcgct gggttcggtg
     3961 gacgccagcc cggcatttcg tcagataacg gaactggtga aatcccagtc acgcgatggc
     4021 gacccggact ttgcggctta ttataaagag ccgtcgaaaa ctatccctga cccgaaactc
     4081 aacctggttt atatctacgg cgaaagtctc gagcggacct attttgataa cgaagctttc
     4141 ccggatctca cgcctgaact gggcgcgttg aaaaatgaag gcctggattt cagccacacg
     4201 cagcagctgc cgggaacaga ttacacgatt gcgggcatgg tggcttctca gtgcggcatt
     4261 ccgctttttg ccccatttga aggtaacgcc tccgcttctg tttccagctt cttcccgcag
     4321 aacatctgtc tgggcgatat cctgaaaaac tcgggttatc agaactattt cgtgcagggc
     4381 gcgaatctgc gttttgccgg taaagatgtg ttcctgaagt cgcatggctt cgaccactta
     4441 tacggctcag aagagctgaa aagcgtggta gccgacccgc actatcgcaa cgactgggga
     4501 ttctacgacg ataccgttct cgatgaagcg tggaaaaagt ttgaagagct ttcccgctca
     4561 ggtcagcgat tctcactgtt taccctgaca gtcgataccc atcacccgga tggttttatc
     4621 tcgcgtacct gtaaccgcaa aaaatatgat tttgacggta agccgaacca gtcattcagc
     4681 gcggtaagtt gcagccagga gaatatcgcg gcgtttatca acaaaatcaa agcgtcaccg
     4741 tggtttaaag atactgtcat cgtcgtctct tctgaccatt tagcgatgaa caacacggcg
     4801 tggaaatacc tcaataagca ggatcgcaat aacctgtttt ttgtcattcg tggcgacaag
     4861 ccgcagcaag agacgctggc agtgaagcgt aacacgatgg ataacggcgc gacggtgctg
     4921 gacattctcg gtggcgataa ctatctcgga cttggtcgta gcagtttatc cgggcagtca
     4981 atgtcggaaa tcttcctcaa tatcaaagag aaaacattgg cgtggaagcc ggatatcatc
     5041 cgcctgtgga aattccctaa agagatgaaa gagttcacca tcgaccagca gaaaaacatg
     5101 attgccttct cgggtagcca tttccgtttg ccgctgctgt tgcgggtttc agacaaacgc
     5161 gtggaaccgc tgccggaaag cgaatactca gcaccgctgc gtttccagct ggccgatttc
     5221 gctccacgcg acaatttcgt ctgggttgac cgttgctaca agatggcaca actctgggct
     5281 ccggaactgg cactttccac cgactggtgt gtctcgcagg ggcagcttgg cggtcagcaa
     5341 attgttcagc atgttgacaa aacaacatgg cagggcaaaa cggcatttaa agatacggtc
     5401 atcgacatgg cgcgttacaa aggcaatgtc gatacgctga agattgttga taacgatatt
     5461 cgctacaaag ccgacagttt catcttcaac gtcgccggtg cgccagaaga ggtgaaacag
     5521 tttagtggga tttcccgtcc agagtcgtgg ggccgctggt ccaacgcgca gctgggcgat
     5581 gaagtaaaaa tcgagtacaa gcatccgctg ccgaagaaat ttgacctggt gattaccgcc
     5641 aaagcatacg gcaataacgc cagccgtcct attccggtac gcgtaggcaa tgaagaacaa
     5701 acccttgtgc tgggcaatga agtgaccacc accacactgc atttcgataa cccaaccgat
     5761 gccgacacac tggtaattgt gccgccggaa cctgtctcaa ccaacgaagg gaatatcctc
     5821 ggacactcgc cgcgtaagct cgggatcggc atggtggaaa ttaaagttgt ggaacgtgaa
     5881 gggtaattcc tgactgatgt gtaggtcgca taaggtgcga cctacacacc ttctccggcc
     5941 tacaaaacat cattgagaaa cagaaaaaaa cagcgcccaa cacttccccg gacgctgcat
     6001 ggcaacttta ataatctcgc cttatcagaa agtaattaca cctttaatta actcacgatt
     6061 gttaatcaca tcgcgctcgt aggtttctgc cagcgtggcg aacggatagc gatgggttaa
     6121 catcatgtaa gcagtgattt ttccttccgc catcagtcga ccgactttag caaaatcttc
     6181 cggcgtggcg ttgcggctgc ccatcatcgt cgtttctttt ttatggaatt ccggatcgga
     6241 gaactgcaac tcacctttaa acaggccgac aaataccacc gtgccgccgt gacgaatcag
     6301 attcacggtg ttattcatcg catgttgatt acctgtcgcg tcgatcactt tctgcgccag
     6361 cgaaccacca aactgcgccc gcaactgcgc gtcaaaatct tcggctgacg ggtccagcac
     6421 gggcaactcc agacgcgttg ccacatgttc acggcgcgcc ggactggtat ccgccaccac
     6481 cacctgtgcg ccatcggctt tagcgattgc cgccgcgccc agaccgattg gccccgcccc
     6541 gaccaccagc acctgctcgc cgggagcaat ggctgcgcga cgcaccgcat gagcgctaat
     6601 agcgaaaggt tcaatcaatg cggccgcctg cgggtcaata ccgtctgcgg gcaaaatgtt
     6661 cgccaccggc acgctcaaat actcactaaa accgccatcc tgatgcacgc caatgactga
     6721 aattttttca cagcaattgg tacgcccgct tttacacgcc gggcattgct gacaagaaat
     6781 atacggaatt accgcaactt gctgtccgat attaaaattg tgaaagtttt ttcctgattc
     6841 aataatctcc ccacatattt catggcctaa aacgcgtgga tagctaaaaa atggttgatt
     6901 gcccgcccag gcatgaatat ctgtaccgca aatcccgacg gttttaattt taattaatgc
     6961 tt
//
LOCUS       DAZGLJ010000235         6925 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000235 DAZGLJ010000000
VERSION     DAZGLJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.235"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..216)
                     /locus_tag="ACHK49_004202"
     CDS             complement(<1..216)
                     /locus_tag="ACHK49_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264735.1"
                     /translation="MTKSWSVPFPESETKHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQE"
     gene            417..911
                     /locus_tag="ACHK49_004203"
     CDS             417..911
                     /locus_tag="ACHK49_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264736.1"
                     /translation="MDVLSGITAAKQAYDLLKTIKETRDDAVIAKAIGDLHQRITDLQ
                     MLNAELSGLYQAEKEIAMKLRDENRKIKMFVVQAENYELHTTEGGSVVYRPKSHSDPS
                     IQQHNLCAHCFGEHKISILQPSTVTIKSNGFFVHSCPRCKNEYRMYRAPDPKPVYVPP
                     LTNY"
     gene            complement(942..1304)
                     /locus_tag="ACHK49_004204"
     CDS             complement(942..1304)
                     /locus_tag="ACHK49_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination N domain protein"
                     /protein_id="HFO9264737.1"
                     /translation="MGQEEKYELKKLIGEDAIEEIAALTTAIKNIRYALNTLISSCDK
                     NSREFLILGAALGIVDAATLHLITHDDILIEPYETLLLVRQKMADAAANGDLQLYIDL
                     RKVLRRMVKTEGDIPLTK"
     gene            complement(1307..1579)
                     /locus_tag="ACHK49_004205"
     CDS             complement(1307..1579)
                     /locus_tag="ACHK49_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264738.1"
                     /translation="MTVVITYLADDNARNRRRARRQAQREQAMQEQRLARKIALKLSG
                     CVRADKAASLGSLRCKKADECSGSICLPNVAIYAAGYRKSKQLTAR"
     gene            complement(2013..2309)
                     /locus_tag="ACHK49_004206"
     CDS             complement(2013..2309)
                     /locus_tag="ACHK49_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264739.1"
                     /translation="MEFLIVFVVVLVIILFVLLSISKKLSQMIEHSSNRAKEEEHIID
                     IKEILSDIKITLDEIKYTTDLIEQYKIPTPNERKAIDQYRIDLEIDEMLSKRKD"
     gene            complement(2350..3057)
                     /locus_tag="ACHK49_004207"
     CDS             complement(2350..3057)
                     /locus_tag="ACHK49_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO9264740.1"
                     /translation="MKTFAERLNAAMSSAGVSQSQLADMVGISQPAIQKMSSGKTNGS
                     RKMVELANALKVRPEWLSSGIGEMRDGAHEEPSNVRDSSLKAVVWEDIKRNDDEFVAL
                     PLLNVSLSAGSGSCELEESSEFSLVFRKHYLKKMGVSERSAKLVRVVGQSMEPTLHDG
                     DVVGVNTQDTTIRDGKTYAICQSDLLRVKTLIATPTSVIIRSINREEYPDEVMDRDEF
                     HETVRIIGRVFWSSHSW"
     gene            3169..3393
                     /locus_tag="ACHK49_004208"
     CDS             3169..3393
                     /locus_tag="ACHK49_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9264741.1"
                     /translation="MNLVIQRALKIVGSQKRLADKCGVTQPAVHKWLKGGLVSPEKVT
                     AIVNATGGQIKAYEIRPDLPHLFPKPNQAA"
     gene            3503..3781
                     /locus_tag="ACHK49_004209"
     CDS             3503..3781
                     /locus_tag="ACHK49_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFO9264742.1"
                     /translation="MELTSTRKKANAITSNILNRIAVRGQRKVADALGINESQISRWK
                     DSFIPKMAMLLAVLEWGVEDEELAELAKKVAMVLTKEKPQDCGNSFEA"
     gene            3817..3978
                     /locus_tag="ACHK49_004210"
     CDS             3817..3978
                     /locus_tag="ACHK49_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264743.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            3965..4786
                     /locus_tag="ACHK49_004211"
     CDS             3965..4786
                     /locus_tag="ACHK49_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO9264744.1"
                     /translation="MGVVKLADYRPQLEVVEHRVADTEDGFMRVANEIIDSLLMADLT
                     VRQMKVMLAIMRKTYGFNKPMDRLTNTQIAAMTGIHHTHVCAAKRQLIERKFLIADGV
                     KIGVNKVVSQWISQDSLTLAKTANKTLAESANGYKPSQLNTKDNIQKTINTNTPLPPN
                     GGGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQLKTP
                     NVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA"
     gene            4783..6159
                     /locus_tag="ACHK49_004212"
     CDS             4783..6159
                     /locus_tag="ACHK49_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO9264745.1"
                     /translation="MRQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDTSSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIEELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERNKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGPLGTVYQRFCNGHFVACDQDEARQICTASNAPASRGR
                     RYAQGADV"
     gene            6246..6461
                     /locus_tag="ACHK49_004213"
     CDS             6246..6461
                     /locus_tag="ACHK49_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264746.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPIDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDLK"
BASE COUNT         1871 a         1506 c         1645 g         1903 t
ORIGIN      
        1 ttcctgtaac cactgatttg gtgctggttt aaaattaaca atccaatgcg caggaaccaa
       61 ccatgcataa tgctctgtct gatgaaaagc tatatattga agtgcgaata ttttgattcc
      121 atcttcttca actgtcgcct ggaatctcca gaaaacaggc attccatcat gtttagtttc
      181 tgattcagga aaaggtacgc tccatgattt tgtcatatct cacctcaaat aagtggtttg
      241 ctgtctaatt tcattttctg gcgaccaaca caagtcacac ccatttcact gcgtggcttg
      301 cggtagtaaa gattgtgcct gtcttttaac cacatcaggc tcggtggttc tcgtgtaccc
      361 ctacagcgag aaaaatagta aaatcctctt acccctacag taagagagta atttatatgg
      421 atgtgttatc tggtataact gccgcgaaac aggcttatga tctgctgaag acaataaaag
      481 aaaccagaga cgatgctgtt atcgctaaag ctattggtga tctacaccaa agaataactg
      541 acttacagat gcttaatgcg gagctctctg gcctttatca ggccgagaag gagatcgcaa
      601 tgaagcttcg agatgaaaat agaaaaatca agatgtttgt tgtgcaagct gagaattatg
      661 aacttcatac aactgaaggt ggttctgtcg tgtatcgacc taagagtcat tccgatcctt
      721 ctattcagca gcataacctt tgtgcacatt gcttcggcga acataaaata tcgatacttc
      781 aaccaagcac ggttactata aaatctaatg ggttctttgt gcattcatgt cctcgctgta
      841 agaatgaata ccggatgtat agagcgccag accctaagcc tgtatatgta ccgccgctga
      901 caaattatta attttattcc tgaaatggcc tcttcacccc cttactttgt cagggggata
      961 tctccttcag ttttgaccat tcgccttaat actttcctta agtcgatgta aagttgaagg
     1021 tctccatttg ctgcggcatc agccattttt tgcctgacaa gcagtaatgt ttcatacggc
     1081 tcaataagaa tatcgtcatg agtaattagg tgaagcgttg ccgcatcaac tattcctaga
     1141 gctgcgccaa gtatcaaaaa ttccctgcta tttttgtcgc atgaggagat aagcgtattt
     1201 agcgcatacc taatattctt tatagctgtt gttaatgctg caatttcttc tatggcgtct
     1261 tctccaatga gctttttaag ctcatatttt tcttcctgac ccataattac ctcgccgtca
     1321 gttgttttga tttccggtag cctgccgcgt aaatggctac gtttggcagg caaatacttc
     1381 cactgcattc atctgccttc ttgcagcgaa ggcttccgag tgatgctgct ttatctgctc
     1441 tgacgcaacc agagagcttt agcgcaattt ttcgtgccaa tcgctgttct tgcattgcct
     1501 gttcacgttg agcctgtctg cgtgctctgc ggcgatttct ggcgttatcg tcagccagat
     1561 atgtaatgac tactgtcatg tttacctccg atgattgact ttggcggtga cgcgccgcgt
     1621 gcttatcttc cggttgccgt cttgcagctg cacttcacgt caccccaaag ccaactactc
     1681 tttggttccc gcatttcggc gggacaatcc catcaatgtt aaagagcctg ccaatctgtt
     1741 ccgtttggct tccagcgtcc tgctgatggc ttaaagataa cttaggttat aggtgtggtc
     1801 aataacctaa tttatatttt atggtaaata ggttataagt gatggataac aaaggtattt
     1861 tatttttgta aatgttgctg attgattggt gtttgagggc ttgcgtgcgg ggtgaaggtg
     1921 ttacctttgg cttgatgctt gtctatgatg aggatggttg attgggtggt gaacggcagg
     1981 aaaagaaaac ccggcgctga ggccgggtgt ttttagtctt ttcttttgct tagcatttcg
     2041 tcgatttcta agtcaatacg atattgatct attgcttttc tctcgtttgg ggttggtatt
     2101 ttatactgtt caattagatc tgttgtgtat tttatttcat ctaatgtgat ttttatatca
     2161 gagagaatct cttttatatc aattatatgc tcttcttctt ttgcacgatt agagctatgt
     2221 tcaatcattt gagatagctt tttactaatg cttagcaaaa caaaaagaat gatgaccaaa
     2281 acaacaacaa aaactatcag aaattccatt atccctccgc acttccgtaa gtcttcttct
     2341 gattatcggt taccaactat gagacgacca gaatactctg ccaataatcc ttacggtttc
     2401 atgaaattca tctctatcca ttacttcatc cgggtactct tcgcgattta ttgatctgat
     2461 tatcaccgat gtaggggtgg cgattaatgt ttttactcgt aacaaatcag actggcaaat
     2521 agcgtaggtt ttaccatctc tgattgtggt atcttgcgtg ttaacaccaa caacatcgcc
     2581 atcgtgaagc gttggttcca tgctttgccc tacaacccta actagcttgg ctgatctttc
     2641 agatactccc atctttttca gatagtgctt tctgaaaacc aaagagaact ccgatgattc
     2701 ctctagctcg cagctaccgc ttccagctga aagcgaaacg ttaagaagag gcaacgcaac
     2761 aaactcgtca tcgtttcttt taatgtcttc ccataccaca gcttttaaag atgaatcacg
     2821 gacattggat ggttcttcat gtgcaccatc cctcatttca ccaataccag aactaagcca
     2881 ttcagggcgc acttttaaag cattggctaa ttcaaccatc ttgcgagatc cgtttgtttt
     2941 accggacgac atcttctgta tggctggctg agatattcca accatgtcag caagctgtga
     3001 ttgtgatacc cctgctgagc tcatggctgc atttagtctt tctgcgaatg ttttcatacc
     3061 cacaaatcta taactacggt tatccaaagt aaaataacaa aggttattgc tgtttttcat
     3121 aacttgagtt atctttggtt ataagtaatg accacaagag gtatgctcat gaatttagta
     3181 attcaacgag ccttgaaaat tgtcggtagc caaaagcgcc ttgccgacaa gtgtggtgta
     3241 acgcagccag cagtacacaa atggctgaaa ggcgggttgg tctctccaga gaaagttacc
     3301 gccatcgtta acgccactgg agggcagatc aaggcttacg aaattcgccc cgatttgcca
     3361 cacctgtttc caaaaccgaa tcaggcagca taagtaacac cgctctttaa cagtcatggt
     3421 cctcattccc gccgaaatgc gggaatacaa cgcgcataag ttgatgcgca taacttctta
     3481 tttgttaagg aaatacttac atatggaact tacaagtact cgcaagaaag cgaatgcaat
     3541 tacaagcaac atcctgaatc gaattgctgt acgtggtcag cgaaaggttg ctgatgcatt
     3601 agggatcaat gaatcgcaaa tttcgcgatg gaaagacagc tttatcccaa agatggccat
     3661 gcttctggct gtgctggagt ggggtgttga agacgaggaa ttagcggagc tggcaaagaa
     3721 agtagccatg gtgctgacaa aagaaaagcc tcaagactgc ggcaacagtt ttgaggcctg
     3781 attgtagaaa gactgtatca atccacagga gtaattatga caaaacgtcg taagaaatac
     3841 caggaaaaag aagagattcg acaccctgat tcacctgagg gattagtggt agccgcagca
     3901 aataacaggg cgttcgcaga gcgccttgtt ggtgtttaca gactagccaa agcaggagtg
     3961 aaacatgggc gtcgttaagt tagctgatta caggcctcaa ctggaggtcg tggagcatcg
     4021 cgtggcagat accgaagatg gtttcatgcg cgttgctaac gagattatcg acagtctgct
     4081 gatggctgat ttaaccgtcc ggcagatgaa ggtgatgctc gctatcatgc gcaagacata
     4141 cggattcaat aagccgatgg atcgactcac aaacacgcag atagcagcca tgacaggtat
     4201 tcatcacact catgtttgcg ctgccaagcg ccagcttatt gagcgtaaat tcctcattgc
     4261 tgatggcgtg aaaatcggag tgaacaaggt ggtttctcag tggattagcc aggacagctt
     4321 aacattagct aaaacagcta ataaaacatt agccgagtcg gctaatgggt ataagccaag
     4381 tcagctaaac acaaaagaca atatacaaaa gacaataaat acaaataccc ccttaccccc
     4441 taacgggggc ggcgatgggc aggttaaacc tgaacgtcgc aaggcagaac gaatcgacta
     4501 cgaatccttc ctgaacgcct acaacaccga agtcggtgac agacttccac acgctgttgc
     4561 ggtcaacgag aaacgaaaac gccgcctgaa gaaaatcatc ccgcaactga aaacgccaaa
     4621 cgtggacggt ttcagagcgt atgtcagggc gtttgtgcat caggccaagc cgttttactt
     4681 cggagacaac gacacgggct ggacggcaga ttttgattac ctgctgaggg aagactcgtt
     4741 aacgggagtt cgggaaggga agtttgcaga cagggggatt gcatgagaca ggatatcgaa
     4801 gcgagcgtta tcggtggctt gctgattggt ggattaacac caaccgccag cgacgttctg
     4861 gcaacgctgg agccggaagc gttttcaatt ccgctctacc ggaaagcctt cgaagttatc
     4921 cgtaagcagg cgagaaacag aaacctaatc gacgcgctga tggttgccga ggcgtgcgga
     4981 gaggagcatt tcacgtcaat cctgatgacc agcaaaaact gcccgagtgc cgcaaacctg
     5041 aagggatatg ccggaatggt cgcggataac tatcaccgcc gtctggtgct ggaaatcatg
     5101 gacgaaatgc gtgaaccaat tcagagcgga accatcgata catcgagtca ggcgatggac
     5161 gagcttgtaa agcgtctctc agccatcaga aagccccgtg acgaggttaa acctgtacgg
     5221 ttaggggaaa tcatcactga ctacactgac acgcttgaca ggcgtctgag gaacggagaa
     5281 gagtcagata ccctgaagac cggaatcgaa gaacttgacg ccatcaccgg agggatgaac
     5341 gcggaagacc tggtgattat cgccgctcgt cctggtatgg ggaaaaccga actggcgctg
     5401 aagattgccg aaggtgttgc aagccgcgtt attcctggtt ctgacgtccg gcgcggagtg
     5461 ttgattttct cgatggaaat gagcgcattg cagattgcag agcggagcat tgccaacgcc
     5521 gggaggatgt cggttagcgt actgcgaaat cctgcatcga tggatgacga aggctgggcg
     5581 cgtgttgcta acggcatgag tcagcttgca gatttggatg tatgggtagt cgatgcctcg
     5641 cggttatcgg tagaagaaat tcgctcaatc gcagagcgga acaaacagga aaatccaaac
     5701 ctgtcactca tcatggcgga ttatcttggc ctgattgaga agccgaaagc agaccgcaac
     5761 gacctcgcaa ttgctcacat ctcaggaagc ctgaaggcga tggcgaaaga cctgaaaacg
     5821 cctgttatct ccctaagtca gctttcgcgc gatgttgaga agcgaccaaa caaacgcccg
     5881 acaaacgcag atttgcgtga ttcaggaagc attgagcagg acgcagactc aatcatcatg
     5941 ctctatcggg aagcggtata tgacgagaac agtagcgccg caccatttgc tgaaatcatt
     6001 gtgacgaaaa accggtttgg cccacttggt acggtttacc agcggttctg taacggacac
     6061 tttgttgcat gtgaccagga tgaagccaga cagatttgca cagcatcaaa tgcacctgct
     6121 tcgcgtggca gacgatatgc acaaggggct gacgtatgac catctacatc actgagctaa
     6181 taacaggcct gctggtaatc gcaggccttt ttatttgggg gagagggaat tggaggcttt
     6241 aagaaatgag tacgatagct gagcttgtca gggctaattt tcgtgaagag ttggtgcgtt
     6301 ggtatcggta tcgttcatcg tccagtttgc cgattgatga gttgtatgag cattcacctg
     6361 ctgcacgacg ctatccgcgt gaccgtgtcc ttcgacggtt gttcaaactc aacaatgagt
     6421 ttcagcgcaa cagaataatc cggagtctgg atttaaagtg aaggagtgag catgagtcga
     6481 agaagtagct ttttggggtt tgtaatattc ctgtcctgca ctggttacat cgtaatctgg
     6541 tcaatttcga acattgaccg tggcggggaa tatctcattg taatgttctt tcctttgttt
     6601 cttgggtggt acgccgcaag gttgctggaa gaatggggtt acaggcataa aaaataaagg
     6661 agtgttcagt gaagcaaacc cttttttatt ggattaagag cgaggggtaa gtaccgatgg
     6721 taaatgcatt tatttgtagt ttatttcttg tcgcgatttt tcatggattc cttctgatga
     6781 tgagttttgt tctctggaat aatggatatc gcatattggg agtaggtttt gttttacggt
     6841 tttcagttgt ctgcgcgttg ctaccgataa ttatggcgac tatcaaatat tattggtaac
     6901 ccaaaaaatc atcgatggag agtga
//
LOCUS       DAZGLJ010000236         6918 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000236 DAZGLJ010000000
VERSION     DAZGLJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.236"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..749)
                     /gene="pdeC"
                     /locus_tag="ACHK49_004214"
     CDS             complement(<1..749)
                     /gene="pdeC"
                     /locus_tag="ACHK49_004214"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO9264747.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAG"
     gene            1179..1460
                     /gene="yjcB"
                     /locus_tag="ACHK49_004215"
     CDS             1179..1460
                     /gene="yjcB"
                     /locus_tag="ACHK49_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO9264748.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIELVMIEEMNQTPPQWPMILT"
     gene            complement(1559..2095)
                     /gene="ssb1"
                     /locus_tag="ACHK49_004216"
     CDS             complement(1559..2095)
                     /gene="ssb1"
                     /locus_tag="ACHK49_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO9264749.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            2349..5171
                     /gene="uvrA"
                     /locus_tag="ACHK49_004217"
     CDS             2349..5171
                     /gene="uvrA"
                     /locus_tag="ACHK49_004217"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO9264750.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(5206..5562)
                     /gene="yjbR"
                     /locus_tag="ACHK49_004218"
     CDS             complement(5206..5562)
                     /gene="yjbR"
                     /locus_tag="ACHK49_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO9264751.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(5566..5982)
                     /gene="yjbQ"
                     /locus_tag="ACHK49_004219"
     CDS             complement(5566..5982)
                     /gene="yjbQ"
                     /locus_tag="ACHK49_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO9264752.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(6093..6806)
                     /gene="aphA"
                     /locus_tag="ACHK49_004220"
     CDS             complement(6093..6806)
                     /gene="aphA"
                     /locus_tag="ACHK49_004220"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO9264753.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKLGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         1695 a         1775 c         1773 g         1675 t
ORIGIN      
        1 ccggcgggca acgcaaaaat aagatgatta taaaaatggg ttataagacg ttgatatgaa
       61 gtcgcaacaa tggctgcaat tgggcgtttt gtcgaataaa ctgtcgcata taaatagcca
      121 tttctttgta cggttaaatc cttcaaatga atcagcggag aaaacgttgc tgccgaggcc
      181 tctttgctta acgagaaaaa ggttttcgtc accgtatcat acacacccca ttgcaatgtc
      241 ggatcatcag acatcacttc actccagaag agagggttga taaccgccac ataatttccc
      301 cgctgcatat aggtcatttt atagccagag aaaaaaggcg tatcgcggta ataatagata
      361 gaaacgttag gttcacgctt ataatcggcc ggtgcaatcg tatagccgtt tacaggcgct
      421 atcagcgatg agcataaaaa atggttatca cgggcataga tcaattcatt aatataaaga
      481 tagccacgaa taatattcaa cattcgcttt tgatgggctg gagtgcaaac ttgcccctga
      541 tagcgttcag ctgcatcgct cactaaatct gcctggcgaa tgaccagctc ggatttatcc
      601 agtgccagtt gagcaaaggt tcggagctga ttattcactt ctgattttgc ccaaaggaag
      661 gcaatccata gcgaaagaat gatgggaaag agaactaaaa agataatgcc cggcaacgcc
      721 agtaattggt gtcgtgcacg atgactcatt gctatcaatt tcctgcctgt ttatacccgg
      781 ccattgcaag gtgaaaaccc tgggtaccga cgtctttaga taaatcttac ggtataaata
      841 gcacttttgc ctgatttgaa attgatacct ttctaatcgt cacctggatg aaaataattc
      901 attgatattc aaaattaatt tcctacagga atgcagaggc ggcgggaaga tatgccctta
      961 atgccactga aaaaccacat aatctgtaca gcaataaaag tcacggccta atatggctaa
     1021 caaacaatca attgtgatat agttcacaaa attaatgaaa caaacagagt gtttcatttt
     1081 tgtgttctgc aagccccgcg atagccagag tgtaccacgc ctcccgtgaa caacgccgcg
     1141 ctgtccaggg catcggctct tttcagagga attgtttgat ggctaccctc accactggcg
     1201 tggttcttct tcgctggcaa cttcttagtg ccgtaatgat gtttctggcc agcacactca
     1261 acatccgttt tcgtcggtct gattatgtcg ggcttgcggt gatcagcagc ggtctgggcg
     1321 ttgtttccgc ctgttggttc gcaatggggt tgcttggcat cacaatggcg gacatcaccg
     1381 ctatctggca caacatcgag ttggtgatga tagaagagat gaatcagaca ccgccacaat
     1441 ggccaatgat tttgacctga tacgtagaag cctccataac ggaggcttct tttttatggc
     1501 tggcagatgc tttaatcatc caccttaaaa caatataacc tattgtttta atgacaaatc
     1561 agaacggaat gtcatcatca aagtccatcg gcggctcgtt agacggcgct gccggagcgg
     1621 actgctgcgg gcgagactgc gcgccgccgc tgaactgatt gccaccctgc ggctgctgtg
     1681 gctgacccca accgccctgc ggctgaccac caccgatatt gccacctgcc ggagcgccac
     1741 caccctgacg accacccagc atctgcatgg tgccgccaac gttcaccacg acttctgtgg
     1801 tgtagcgatc ctgaccggat tgatcggtcc atttacgggt acgcagctga ccttcgatat
     1861 aaacctgaga acctttacgc agatattcgc tcgccacttc tgccagtttg ccgaacagca
     1921 caacgcggtg ccattcagtc tgttctttca tctcgccggt cgctttatca cgccaggatt
     1981 cggaagtagc cagcgtaatg ttggcaactg cgccaccatt tggcatgtag cgtacttccg
     2041 ggtcctgacc cagattacca acgagaataa ccttgtttac gcctctgctg gccatgttcg
     2101 tgtctcctga aaaaaatcgt tctgaataag tgtaaacgcg cgattgtacc attaccaata
     2161 gcgcttttac tatgttgtga cctcggttcc gggaaacaaa cctggccaga cattgttaca
     2221 caacactccg ggtaatgcat tccaatactg tatattcatt caggtcaatt tgtgtcataa
     2281 ttaaccgttt gtgatcgccg gtagcaccat gccaccgggc aaaaaagcgt ttaatccggg
     2341 aaaggtgaat ggataagatc gaagttcggg gcgcccgcac ccataatctc aaaaacatca
     2401 acctcgttat cccccgcgac aagctcattg tcgtgaccgg gctttcgggt tctggcaaat
     2461 cctcgctcgc tttcgacacc ttatatgccg aagggcagcg ccgttacgtt gaatcccttt
     2521 ccgcctacgc gcggcagttt ctgtcactga tggaaaagcc ggacgtcgat catattgagg
     2581 ggctttctcc tgccatctca attgagcaga aatcgacgtc tcataacccg cgttctacgg
     2641 tggggacaat caccgaaatc cacgactatt tgcgtttgtt gttcgcccgc gtcggcgaac
     2701 cgcgctgtcc ggaccacgac gtcccgctgg cggcgcaaac cgtcagccag atggtggata
     2761 acgtgctgtc gcagccggaa ggcaagcgtc tgatgctgct cgcgccaatc attaaagagc
     2821 gcaaaggcga acacaccaaa acgctggaga acctggcaag ccagggttac atccgtgctc
     2881 gtattgatgg cgaagtctgc gatctttccg atccgccgaa actggaactg caaaagaaac
     2941 ataccattga agtggtggtt gatcgcttca aggtgcgtga cgatcttacc caacgtcttg
     3001 cggagtcgtt tgaaaccgcg ctggagcttt ccggtggtac agcggtagtg gcggatatgg
     3061 acgacccgaa agcggaagag ctgctgttct ccgccaactt cgcctgccca atttgcggct
     3121 acagtatgcg tgaactggag ccgcgactgt tttcgtttaa caacccggca ggtgcctgcc
     3181 cgacctgtga cggccttggc gtacagcaat atttcgatcc tgaccgcgtg atccaaaacc
     3241 ccgagctgtc actggctggc ggtgcgatcc gtggctggga tcgccgcaac ttctattact
     3301 tccagatgct gaaatcgctg gcagatcact ataagttcga cgtcgaagcg ccgtggggca
     3361 gcctgagcgc gaacgtgcat aaagtggtgt tgtacggttc tggcaaagaa aacattgaat
     3421 tcaaatacat gaacgatcgt ggcgatacct ccatccgtcg tcatccgttc gaaggcgtgc
     3481 tgcacaatat ggagcgccgt tataaagaga cagaatccag tgcggtacgt gaagaattag
     3541 ccaagtttat cagcaatcgc ccatgcgcca gctgcgaagg gacgcgtctg cgtcgggaag
     3601 cgcgccacgt ttatgtcgag aatacgccgc tgcctgctat ctccgacatg agcatcggtc
     3661 atgcgatgga attcttcaac aatctcaaac tcgctggtca acgggcgaag attgcggaaa
     3721 aaatccttaa agagatcggc gatcgtttga aattcctcgt taacgtcggc ctgaattacc
     3781 tgacgctttc ccgctcggca gaaacgcttt ccggcggtga agcccagcgt atccgtctgg
     3841 cgagccagat tggtgcgggc ctggttggcg ttatgtacgt gctggacgag ccatctatcg
     3901 gcctgcacca gcgcgataac gagcgcctgt tgggtacgct tatccatctg cgcgatctcg
     3961 gtaataccgt gattgtggtg gagcacgacg aagacgcaat tcgcgccgct gaccatgtga
     4021 tcgacattgg cccgggcgca ggtgttcacg gcggtgaagt ggtcgcagaa ggtccgctgg
     4081 aagcgattat ggcggtgccg gagtcgttga ccgggcagta catgagcggc aaacgcaaga
     4141 ttgaagtgcc gaagaaacgc gttccggcga atccggaaaa agtgctgaag ctgacaggcg
     4201 cacgcggcaa caacctgaag gacgtgacgc tgacgctgcc ggtgggtctg tttacctgca
     4261 tcaccggggt ttcaggttcc ggtaaatcga cgctgattaa cgacacactg ttcccgattg
     4321 cccaacgcca gttgaatggg gcgaccatcg ccgaaccagc accgtatcgc gatattcagg
     4381 ggctggagca tttcgataaa gtgatcgata tcgaccaaag cccaattggt cgtactccac
     4441 gttctaaccc ggcgacctat accggcgtgt ttacgcctgt gcgcgaactg tttgcgggcg
     4501 taccggaatc ccgtgcgcgt ggttatacgc caggacgttt cagctttaac gtccgtggcg
     4561 ggcgctgcga agcctgtcag ggcgacggtg tgatcaaagt agagatgcac ttcctgccgg
     4621 atatctacgt gccgtgcgat cagtgtaaag gtaaacgcta taaccgtgaa acgctggaaa
     4681 ttaagtacaa aggcaaaacc atccacgaag tgctggatat gaccatcgaa gaggcgcgtg
     4741 agttctttga tgccgtacct gcactggcgc gtaagctgca aacgttgatg gacgttggcc
     4801 tgacgtacat tcgcctgggg cagtccgcaa ccacactttc tggtggtgaa gcccagcgcg
     4861 tgaagctggc gcgtgagctg tcaaaacgcg gcaccgggca gacgctgtat attctcgacg
     4921 agccgaccac cggtctgcac ttcgccgata ttcagcaact gctcgacgta ctgcataaac
     4981 tgcgcgatca gggcaacacc attgtggtga ttgagcacaa tctcgacgtg atcaaaaccg
     5041 ctgactggat tgtcgacctg ggaccagaag gcggcagtgg cggcggcgaa atcctcgtct
     5101 ccggtacgcc agaaaccgtc gcggagtgcg aagcttcgca tacggcacgc ttcctcaagc
     5161 cgatgctgta atcgttaagg ccgctttctg agcggccttt tcctttcaga gttgtaccag
     5221 caatttacgc ttttcttccg gtagtaaatt cactgcctgc tgataagacg catccaccag
     5281 ataatagatt tgcgaatccg gcagcgaacc gtcgagatac acggtgctcc agtgcgcttt
     5341 attcagatgg cggctaggac gcacatcgct gtgctgctga cgtagcagct ccgccagctc
     5401 cgggctggtt ttcagcgaaa cagctgggcg attttctact tctttcacca tcgcaaacag
     5461 tacatcttcc actttgatct gcgtcgcttt ccagtcgtta tgcacgctct gttctgcgcc
     5521 tggttttgcc atgcaatatt gtagcaactc cgaaatggtc attttttact ccccttgtag
     5581 tgtcgcgatg atgcgacgcg atccgccgtg gatgcgatgt tcccccagcc agatgccttg
     5641 ccaggtgccg gtctgaatac gccctttatg taccggcaat acaagcgatg ttcccagcat
     5701 tgaggatttg atatgagaag gcatatcgtc tgctccctca tagtcatgct cataatttcc
     5761 gttgtcggga acggtgcgga ggaaaaaacg ctccatgtcg tggcgtacgg tgggatcgca
     5821 gttctcatta agtgtcagag aggcggaggt atgttgcagc aacagatgca gtaagccgat
     5881 gttaacgcgc ggcatatcag ccagctgatt cagaatttca tccgttacca gatgaaaccc
     5941 acgagatttg gcgctaagcg tgagcgtctt ttgataccac atgtgctgct ccttgataaa
     6001 actctcttaa tcagtttgca gcaagacagc gaaaggataa aggtgtgatt aaaaaaacag
     6061 cattcgggag agcgtcacgc tctcccgctc tgtcagtatt ctgaattgac gatcacctct
     6121 tcaccaaacg cacccgcttg tggcaagggt ttgtaggtag agttggaggc gcgcagaatg
     6181 cggataccac gagcgccgac atcgcgtgcg gcggtaatat cattatcaga atcgccataa
     6241 aaaattcgga tatttttatc ctgcagccat tgcgattttg tattttgccc tagtttatcg
     6301 cccgcaaaga tcaccggatt catgttggtg gcaggaatat gaaaattatc cgccagcgtt
     6361 tttgaaaccg tttctgtttt cgtcggacta cgaccagtca caaagaagat cgcgtcaccg
     6421 cggcgtacat gcatatcaat cagctggcga gcgacctctt ttggaatgct gaattcatcc
     6481 cagccattgt tcattttttc ccagaacaca ggatttttca gataatcttc gctttctggc
     6541 gagaaggttt ttttgccgcg ccagaagccc ggactggaaa aaagtaccgt gtcatcgata
     6601 tcaaacccca ccgccattgg cggacgccct gcgaggctat tttcaatttg tgcgaccgaa
     6661 acccaatgaa tgggtgcctg ttcagcaagc ctggcaacgt tagtcccagg gttaagcggt
     6721 gaaggagatg aggccagggc aacagcggaa ctgtttagag cgaacaataa gcaaacggca
     6781 ctgattgcct gtgtgatctt gcgcatattt ttccctaaat agtcagtatg ttgaaacttt
     6841 ttattgtgag atttgttgca aaaaccatct gaccataacg ccagctgccg gggaaaggaa
     6901 ggattatctg cggttttt
//
LOCUS       DAZGLJ010000237         6917 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000237 DAZGLJ010000000
VERSION     DAZGLJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.237"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            148..510
                     /locus_tag="ACHK49_004221"
     CDS             148..510
                     /locus_tag="ACHK49_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264754.1"
                     /translation="MTQNVLPINKSLHDRAVDEFNRLHGTMIGEISAMLKTAKVAPLV
                     DLRKKDPTFSNVVAELRTFRDVCNALLPYFRVDKTSEIAVIDKLLILANDLAQAIDAD
                     DPDALCAAIAALDVEPYI"
     gene            526..654
                     /locus_tag="ACHK49_004222"
     CDS             526..654
                     /locus_tag="ACHK49_004222"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264755.1"
                     /translation="MTKAFDYTAVSKLLAEMRGCVERVQNLRRDFEAHINHSQKAA"
     gene            920..1139
                     /locus_tag="ACHK49_004223"
                     /pseudo
     CDS             920..1139
                     /locus_tag="ACHK49_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1117..1389
                     /locus_tag="ACHK49_004224"
     CDS             1117..1389
                     /locus_tag="ACHK49_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFO9264756.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQEAISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVAIYAAGYRKSKQLTAR"
     gene            complement(1413..1952)
                     /locus_tag="ACHK49_004225"
     CDS             complement(1413..1952)
                     /locus_tag="ACHK49_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="super-infection exclusion protein B"
                     /protein_id="HFO9264757.1"
                     /translation="MSNWWQELLRFFLRGLTLQQLIHMLIILIALIIITPASIKEWVD
                     IRNPEILPDHWMYYAMLLCISYVLNRGMEFIFLASSDRYKKYLSKRDEAKVIVETERL
                     FNSLSIQEKEVLAFAVMANNKIVLKHGDPVALSLMRKGLLHRSGVTYSASGKEKFVIP
                     DVWFHECYMRFAGKADELI"
     gene            2208..3176
                     /locus_tag="ACHK49_004226"
                     /pseudo
     CDS             2208..3176
                     /locus_tag="ACHK49_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
     gene            3201..3332
                     /locus_tag="ACHK49_004227"
     CDS             3201..3332
                     /locus_tag="ACHK49_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO9264758.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVEVLLCAMK"
     gene            3317..3469
                     /gene="kil"
                     /locus_tag="ACHK49_004228"
     CDS             3317..3469
                     /gene="kil"
                     /locus_tag="ACHK49_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO9264759.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PCYRRK"
     gene            3466..3624
                     /locus_tag="ACHK49_004229"
     CDS             3466..3624
                     /locus_tag="ACHK49_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264760.1"
                     /translation="MSFTDNWSDEEFIRQMKELIGNEGDIHVTCNHSEGEQVTEAHVH
                     AESSLVSP"
     gene            3726..4331
                     /locus_tag="ACHK49_004230"
     CDS             3726..4331
                     /locus_tag="ACHK49_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFO9264761.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAAMQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
     gene            4331..4714
                     /locus_tag="ACHK49_004231"
     CDS             4331..4714
                     /locus_tag="ACHK49_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264762.1"
                     /translation="MAHSITVRLNKPAREFQTGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVVFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     ASGNGPQQQSSNQQNTPVYDDSIPF"
     gene            4738..5031
                     /locus_tag="ACHK49_004232"
     CDS             4738..5031
                     /locus_tag="ACHK49_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFO9264763.1"
                     /translation="MPAPLYGADDARRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            5042..5206
                     /locus_tag="ACHK49_004233"
     CDS             5042..5206
                     /locus_tag="ACHK49_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFO9264764.1"
                     /translation="MRGLAYNPGILPAEMIIRQRAKPMPSREELLKRKSFGSVNDNKY
                     LNAMWRSGKK"
     gene            5203..5760
                     /locus_tag="ACHK49_004234"
     CDS             5203..5760
                     /locus_tag="ACHK49_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264765.1"
                     /translation="MKQMTLIEMDGFLKGKCIPSDLKVDETNDEYLVRKFGELESKLE
                     TALRECRSAGITIDNLEAKCAKMAAENSSLKQSEKEFNDFCREEFSEWEDDITETPAT
                     DAFLAEVKTEARKEGAYFVANRMLAAWEAGFIDDTAKNAADIARMILTSTEFMANAPE
                     GDFDRSFSDGVLEDIAEQLRKGVIQ"
     gene            5757..6314
                     /locus_tag="ACHK49_004235"
     CDS             5757..6314
                     /locus_tag="ACHK49_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO9264766.1"
                     /translation="MSKIDYQALREAAERAIPAMERLLMLPVDDDLLTEQELKDYGVD
                     IDALNAFKFLTGPETVLALLDERERNQQYIKRRDQKNEDIALTVGKLRVELEAVQKTS
                     AARIEAIDRTHKMFQREKDRADAAEKCIAELSASHSKLRDTMAGIHNTIRMDGGYTPL
                     AAILNAAKRAYEESASAAGIRIKGE"
     gene            6316..6453
                     /locus_tag="ACHK49_004236"
                     /pseudo
     CDS             6316..6453
                     /locus_tag="ACHK49_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2206 a         1484 c         1639 g         1588 t
ORIGIN      
        1 tatgtgtata aatagttaaa aatgcacgat gtagagatat ttgtgcttaa catcaattaa
       61 tcattgctaa atcgtgctta ttgttcagaa atcgcccaag tcatattgaa aatctgttgg
      121 cgaacaccta tataaggagt atagttagtg acccaaaacg tattgccaat caacaagagc
      181 ttgcatgacc gagctgttga tgaatttaat cgtctgcatg gaacaatgat tggcgagatt
      241 agtgcaatgc tgaaaacagc taaagtagcg ccattggtgg atcttcgtaa gaaagatcct
      301 accttttcga acgttgtcgc agaattacgg acattcagag acgtatgtaa tgctctgctc
      361 ccatactttc gtgtagataa gaccagcgaa atcgctgtca tcgataagtt acttatactg
      421 gctaatgacc tggctcaagc catcgatgct gacgatcctg acgcattgtg cgcagcaata
      481 gcagcccttg atgttgagcc ttacatttaa acgaggggaa aaaaaatgac taaagcgttt
      541 gactatactg ccgttagtaa gcttttggct gaaatgcgcg gatgtgtaga gcgcgttcaa
      601 aatctacgcc gcgactttga agctcacatc aatcattcac aaaaagcggc ttgatactta
      661 agttagatga cagaacccgg cctcagcgcc gggttttctt tgcctcacga tccccacacc
      721 taataacacc ttaaccaatt gtatttattg aaaaattgat agatacaact ttctaaacca
      781 cgaaattctg atccccctca agtctcttca ttccttctga taaattcccg caatcaaata
      841 aaaaaccata ataatcaaca ggatatttga aaacccaaac aatttatcat tttgctattg
      901 ccatcagttt atcatttcga taaagtcaac tcatcagcag gacgctgtaa gccaaacgga
      961 gcagactggc aggctcttta aacaacgtcg aacactcgac tacgtggctg aaaagccaga
     1021 tcacccaacc acataagctg tgggatgcaa tgccgaagta accgtctcag gaggagcttc
     1081 gagattgcat cgccaaagtt tattcgggag gaatctatgt ccagaaaaac agaatttaaa
     1141 ggcaccgcag cttctcgccg tagagctcgt cgcgcaaacc tgcaaagtca ggaggcgatt
     1201 agctccgaca agctacacag gccaacccct tcacgagtgg tcttgcaatg caagcgcaaa
     1261 ccagcaatga gagcagaagt tataactctg acaacgttga ccagaaaata tgaaggttca
     1321 acttgtcttc caaacgtagc catttacgcg gcaggctacc ggaaatcaaa acaactgaca
     1381 gcgaggtgac gatccccccc gccgtagagg aactaaatta gctcatcagc tttaccagca
     1441 aagcgcatat aacactcatg gaaccagacg tcaggtataa caaatttctc tttacctgac
     1501 gcgctgtaag tcacacctga gcgatggaga aggccttttc tcataagaga taaagcgacc
     1561 ggatcgccgt gcttaagtac gattttgtta tttgccataa cagcaaatgc taaaacctct
     1621 ttttcctgaa tactcagaga attgaatagg cgctcagtct ccacaattac tttagcctca
     1681 tctctcttac tgagatattt tttatatctg tctgaagatg ccagaaatat aaactccatc
     1741 cccctgttca gaacataact gatacacaaa agcattgcgt aatacatcca gtgatctgga
     1801 agtatttcgg ggttccttat atctacccac tctttaattg atgctggggt gattattatc
     1861 aaggcaatta aaataataag catatgaatt aactgttgta gcgtaagacc acgcaggaag
     1921 aaacgtaata gttcctgcca ccagttactc atcggtgaat ctccatcatt ctctctgtag
     1981 gggtgaatag agtttatccg atttctcgct gtaggggtac acgagaacca ccgagcctga
     2041 tgtggttaaa agacaggcat acgaataaac actgcactgt gtattcattc caacgagtga
     2101 atacacggag caatgtcgct cgtaactaaa caggagccga cttgttctga ttattggaaa
     2161 tcttctttgc cctccagtgt gagggcaatt tttttgacgg aggatatatg agtgaagtaa
     2221 cagatttagt tgttattgaa aaagcaaatg caatgactgt aatttagtct gccgaccaga
     2281 ttgaagaaat tcttcaaaag gttgaacgtg aagttatgtc ctttgtgcct gatatcacaa
     2341 cggcaaaggg cagaaaggag atcgcttatc tggcgtataa agttgcgcag acgaaaacat
     2401 atctcgatgg tcttggcaaa gaccttgttg ctgaactgaa ggaaattcca aagctaattg
     2461 atgctaaccg caagacagtg cgcgatcgcc ttgatgaact gaaagccaag gcgcgccagc
     2521 ctcttactga ttatgaggaa gaacaggcgc ggattaaagc cgaagaagaa gctaaggcag
     2581 cagctgaagc tctcgcaaag caaattgagt ctgaccatga aatagcgatt ttgatggatc
     2641 gcgaatttga ccgccaaaga gaagaggcaa gactcaaagc ggagcaggaa aagcgagagc
     2701 atgaagaacg cttaaaaaga gaagctgaag agaaagccag agctgaagcc gaagcaaagg
     2761 caaaagccga aattgaagca gcagcaaggc gagaagcaga agctaaggcc gcagaggaac
     2821 gtgcagagcg tgaacgcatt gaagccgagc aacgagcaca gcgcgaagca aaagaggcag
     2881 cagaacgagc tgaaagagaa aagcatgcgg caattgaagc agaacgccgt aaagcacagg
     2941 aggaggcaga acgaatcctg cgcgaggctg aagcaaaaga gcaagccaga atagcagaag
     3001 aaaaaagaat caaggacgaa gaagagcgta gagcaaagga taaagctcac cggaaagaag
     3061 taaataacaa aatacttgct gaccttatca aggttggcgc atcagaagat gttgctaaaa
     3121 atatcataac agccatcgta aaaggcgaat tattcgcaac aaaaataacc tactaataaa
     3181 accaacataa ggaaccaccc atgatttacg caatcgcggg aggcgctcgc atgggtgcct
     3241 tccaattaaa tgaatcttta cttgaacgaa tcacccgtaa attacgtgac ggatggaaaa
     3301 gagttgaggt cttattatgc gcaatgaaat agccatcaat caccagatgc ttcgtgctgc
     3361 acagaacaag gcagtaatag cccgatttat tggtgattca aaaatgtggc ttgaagcaaa
     3421 taaagcgatg aaatcagcta tcaaccttcc gtgttatcgc aggaaatgag ttttacagat
     3481 aactggtcag acgaagaatt cattcgtcag atgaaagaat taatcggtaa cgaaggagat
     3541 attcatgtca cttgcaacca cagtgaagga gagcaagtta cagaggcgca tgtacacgca
     3601 gaaagctctc tggtatcgcc ataatggtga ccgcgaagga atgcgggtat gccttaattt
     3661 gtcccgagtc gaagtattaa atcagcgtta tttccttggg ccgtgtccat tctgagaaca
     3721 atcatatgag caaagaattt tacgcaagac tggcagctat tcaggagaat ctgaacgcgc
     3781 caaagaatca gtacaactca ttcggcaaat ataaatacag aagctgcgaa gatattcttg
     3841 aaggcgttaa gccgttactg aatggcctgt ttttatcaat cagcgatgaa gttgtgttga
     3901 ttggtgatcg gtattatgtg aaagccacgg caactattac cgatggcgaa aacagtcata
     3961 cggcaaccgc tcttgcacga gaggaagaaa gcaagaaagg aatggattct gcacaagtta
     4021 cgggagctac aagctcttat gcacgcaagt attgcctcaa tggtttattc ggcattgatg
     4081 atgcgaaaga tgcagatacc gacgagcata aacatcagca gaacgcagca gcaaagcaat
     4141 caaaaccatc acctacacct gaacaggttc taaaagcatt cactgacgca gcaatgcaga
     4201 aaaacaccgt agaagagctt aaacaggcgt tcgccaaagc gtggaagatg ctcgaaggca
     4261 caccggagca gcacaaagcg caggacgttt acaacatcag acgagacgaa ttagaaggag
     4321 ctgctgctta atggcacatt cgattactgt aagactaaac aagcccgcaa gagagtttca
     4381 gaccggggaa aatatcggat tcaacatccg tgctggcgtt cagtattacg atcgccagac
     4441 aaaaaagaaa gaatggacaa actacagcgc cgttgtattt gccaagccgg gagcgcaagc
     4501 ggattactac cgtagtgttc ttgttgaagg tggcattgtg gaaattaccg gagaaaacat
     4561 cagggttgat gtttatcagg ggcaaaatgg tcaatcaatc actcttgaat tactgaatgc
     4621 aaagattgga tttgcagctt caggaaatgg cccgcagcag caaagtagta accagcagaa
     4681 cactcctgta tacgacgatt ccatcccatt ctgatttaga aaaataagga tttaattatg
     4741 ccagcgcctc tgtatggtgc ggatgacgcg cgccgctgtt ccggcaattc cgtatcggag
     4801 gtgctggata aattcagaaa aaactacgat cggataatgt cgctaccgca ggaaacgaaa
     4861 gaggaaaagg aatttcgcca ttgtatatgg cttgcagaga aagaagaacg cgagcgaatt
     4921 taccagacat caatccgacc attccgcaaa gccacatata cccacttccc tgaaattgac
     4981 ccgcgcctgc gtaattaccg ctcacgctat ggcgctatca gtaatgactg aggaatttac
     5041 catgagagga cttgcataca atcccggcat tcttccggca gaaatgatta ttcgccaacg
     5101 cgcaaagcca atgccatcga gagaggaatt gcttaagaga aagagtttcg gttctgttaa
     5161 tgacaacaaa tatctgaatg cgatgtggcg gagtgggaag aaatgaaaca aatgacacta
     5221 attgagatgg atggttttct gaaaggtaaa tgcatcccaa gtgatttaaa ggttgacgaa
     5281 acaaacgatg aatatcttgt ccgtaagttc ggtgaacttg aatcaaaact agaaacggcg
     5341 ttgcgggagt gtcgttctgc tggaatcacg attgataacc ttgaggctaa atgcgcgaag
     5401 atggctgctg aaaattcctc gcttaagcaa tctgagaagg aatttaatga cttttgtcgt
     5461 gaggagttta gcgaatggga agatgatatt actgaaaccc cagccaccga tgcttttctg
     5521 gctgaagtga agactgaagc acgcaaggag ggcgcttact ttgtggcgaa cagaatgctg
     5581 gctgcctggg aagctggttt tattgatgat actgcgaaga acgccgctga tattgcccgg
     5641 atgattctta cctctactga gtttatggcc aatgcgccgg aaggcgattt tgaccgttca
     5701 ttctctgatg gcgttctcga agatatcgcc gaacagcttc gtaaaggagt catccaatga
     5761 gcaagattga ctatcaggca ctgcgtgagg cggcagagcg tgcaattccg gcaatggaac
     5821 gcctgttaat gttgccagtt gatgatgatt tgttaactga acaggaactt aaggattacg
     5881 gtgtggatat tgatgcgctc aacgccttca aatttctgac cgggccagaa accgtgctgg
     5941 cactactgga tgaacgggaa agaaaccagc aatacatcaa acgccgcgac cagaagaacg
     6001 aggatattgc tcttacggtt gggaagctgc gtgttgagct tgaagccgtg cagaagacat
     6061 cagcggcacg aatcgaagct atagaccgca ctcacaaaat gttccagcgt gaaaaagatc
     6121 gagccgatgc cgcagagaag tgcatagcag aactgtctgc tagccacagc aaattgcgcg
     6181 acacaatggc tggcatccac aacacaatcc gaatggatgg cggctatacg ccactggcag
     6241 caatccttaa cgctgctaaa cgcgcatatg aagaatcagc aagcgcagct ggcattcgca
     6301 tcaaaggaga gtgatatgag cactatcact aaagaacgta tcgaattgtt cattaaatcc
     6361 ccgcttgata acggacttac tagtggcgaa caaatggaac tggcacggat tgctctggca
     6421 tcgctggaag caaaaccaat aggtgcattc cacattgcag aacagcaagt tgacggcaca
     6481 agtgactacc tcaaggatgg agaatggcct attgataatg gaattattga ggtctacgcc
     6541 gctccgccag taccggtagt acctgctgca ttacctgaga acgacgatga ggacgggcat
     6601 gacattgatt atcttgatcc atctgaagtt tacgcgcttg ggcgaacagc tggctggaac
     6661 gcctgccgcg ccgccatgct tcagggtaaa tcagaacaac cacaaaacgc acaacaaaat
     6721 attccggaaa atattcccgg tggcaactct ccggtaactc cggatggttg ggttatggtg
     6781 ccgaaggagt taaccccgga aatgatgaga gccgttcaga ttagaagtga gcttggaggg
     6841 tatgctactt ccaatctatc tggtgcatac aatatgtttt ctgagttctg gaatgttgct
     6901 gtctcagctg cgcctaa
//
LOCUS       DAZGLJ010000238         6902 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000238 DAZGLJ010000000
VERSION     DAZGLJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.238"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..183
                     /locus_tag="ACHK49_004237"
     CDS             <1..183
                     /locus_tag="ACHK49_004237"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264767.1"
                     /translation="FNDRLSMNQLPRRALSRYGLTFQGNILSVNCQCRMLTRVRRTHS
                     TSPVENSLITNLSFVG"
     gene            complement(187..405)
                     /gene="ydfC"
                     /locus_tag="ACHK49_004238"
     CDS             complement(187..405)
                     /gene="ydfC"
                     /locus_tag="ACHK49_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO9264768.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(477..776)
                     /locus_tag="ACHK49_004239"
     CDS             complement(477..776)
                     /locus_tag="ACHK49_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264769.1"
                     /translation="MALYIDISAIAGQVRIIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IKLRGLIFSYKVTTIFADGSRETVRAMRFKGCVKDFATTFWARKLDCIHNQFPLE"
     gene            1112..1414
                     /locus_tag="ACHK49_004240"
     CDS             1112..1414
                     /locus_tag="ACHK49_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO9264770.1"
                     /translation="MKIISVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKAQ
                     YRTASILKNRRVVFNIKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            1417..1776
                     /locus_tag="ACHK49_004241"
     CDS             1417..1776
                     /locus_tag="ACHK49_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9264771.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(1823..2215)
                     /locus_tag="ACHK49_004242"
     CDS             complement(1823..2215)
                     /locus_tag="ACHK49_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9264772.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            2342..2602
                     /locus_tag="ACHK49_004243"
     CDS             2342..2602
                     /locus_tag="ACHK49_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9264773.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANAQAAAGVDS"
     gene            2599..3036
                     /locus_tag="ACHK49_004244"
     CDS             2599..3036
                     /locus_tag="ACHK49_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO9264774.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            3123..4133
                     /locus_tag="ACHK49_004245"
     CDS             3123..4133
                     /locus_tag="ACHK49_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFO9264775.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNSDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEAYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHAASGGTAS
                     RAVQEIRAAREQWERENGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEALDCPDWC
                     DD"
     gene            4126..4587
                     /locus_tag="ACHK49_004246"
     CDS             4126..4587
                     /locus_tag="ACHK49_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO9264776.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKLLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            4621..5346
                     /locus_tag="ACHK49_004247"
     CDS             4621..5346
                     /locus_tag="ACHK49_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO9264777.1"
                     /translation="METVFDALKALKRASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMVISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVAETDGKAFPQPAGVALPVREAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKHRDIVRQITDSSRRVVSEK"
     gene            5362..5754
                     /locus_tag="ACHK49_004248"
     CDS             5362..5754
                     /locus_tag="ACHK49_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO9264778.1"
                     /translation="MAKEFTPEEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSGCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQN
                     IICRECRQSEVMQRVLAFYRGNFQEVME"
     gene            5751..6047
                     /locus_tag="ACHK49_004249"
     CDS             5751..6047
                     /locus_tag="ACHK49_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO9264779.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKAEEELKREGHTVLTPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVAR"
     gene            6044..6505
                     /locus_tag="ACHK49_004250"
     CDS             6044..6505
                     /locus_tag="ACHK49_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HFO9264780.1"
                     /translation="MREVNYEALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFK
                     RHIRHREADIIAGLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELDAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRG"
     gene            6483..6839
                     /locus_tag="ACHK49_004251"
     CDS             6483..6839
                     /locus_tag="ACHK49_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264781.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVKLLRQEEQERRKNIEKLIQIE
                     RNAKARIRRVTKSRTE"
BASE COUNT         1858 a         1505 c         1861 g         1678 t
ORIGIN      
        1 tttaacgaca ggctatctat gaaccagcta cctcgccgtg cgctttcgcg ttatggtctg
       61 acttttcagg gaaatatcct ttcagtaaac tgtcagtgcc ggatgctcac ccgtgtccgg
      121 cgcacgcact ccacctcacc cgtggagaac tccttaatta ccaaccttag cttcgttggt
      181 tagctattaa cgcgggtatg taatcattct ggcaatgctt aatgccgctg ctttttccag
      241 attggtgata tcctgctcca gagcggacag attttcagcc tgcttagccc tggcttcatt
      301 ggcccatttc aggtcctgcg ctgccttaat tttctggtgc atccactcat aaagttcatc
      361 atcggtatag tctggcgcga tgatgacggg ttctcgtttc tgcatgtcgg ctccttgtgg
      421 ttagcgctgc ctgcttttaa ccacgtcagg cgaggtggta tcctctgagg ggtctgttac
      481 tcgagaggaa attggttatg aatacaatca agttttcttg cccagaatgt ggtggcgaag
      541 tctttgacac atcctttaaa ccgcatggct ctgacagttt cgcgggagcc atctgcaaaa
      601 attgtggtca ccttgtaact gaagatgagt cctcgcagtt tgatgacgaa atcgttgaca
      661 atatcttcgg tgcactcacc agagactttc tgaagtaaag gcgcataccg cttagttacc
      721 gctctgataa ttcttacctg tccggcaatg gcgctgatat caatataaag cgccatcgct
      781 gtttctttgc tgatccctgg atgccttcca ttctgatgtt tgacttcgcc cactgagaaa
      841 tcctctgttt ccccttaacg ccggggtagc ggaacaaaaa acctgctgca tagttaaacg
      901 taatccccgc cgtcatgttc atacgcctca ggctggctac ttaacccctg accactgccg
      961 ggtaactcga agtattgccc tgcgttctgt ggggcggggt gggtttatgg atacaattta
     1021 caaattaaaa tttaactggt caacatgttt attattaaat ttgtaattgc gggctgttgc
     1081 atcagtccca aaatgggact actatacagt tatgaagatt atctcagtta aaacactcag
     1141 ggatttttgg gcggagaatc ctgatgcaga acaaccgcta agggcatggg tggatgaggc
     1201 ggcaaaagct gactggaaaa gcccagcaga cattaaggca caataccgaa cggccagtat
     1261 attgaaaaac cggcgcgtgg tattcaacat aaaaggtaat cattaccgtt taattgttgc
     1321 aattgcgtat cagcgaggat gggtatttgt taaattcatc ggaaaccaca aggaatacga
     1381 tgctattgat gctgatacca tcgaactgga gtaagcatga acatcaaacc tattcgtaca
     1441 gagcaagatt atgaagccgc gttgcgtgct gttgaaccca tgttcgacaa tgagcccgaa
     1501 attgacacgc ctgaggggga tttttttgag gtgatgtgtt tgctaataga ggaatatgaa
     1561 aaaaaacatt atcccattga gccaccatcc ccaattgaag ctataagatt tcgcatggaa
     1621 cagcaggggc tgactgtgaa agatttggaa cccgcaattg ggaaaaaaaa tcgggtttat
     1681 gaggtgttga atggtaccag aagccttacg ttaccaatga ttcgccgtct tcataatcaa
     1741 tttggtatcc ccctggaaag cctggttgga ttataaaatc tgctagtcat ttgcctgatg
     1801 ctcgttccag aaaaggaatg catcaggcag ttttgttttt ctgccgcagt aactcttcaa
     1861 gtttccgttt atagaaatcg cgtttttgct ccatatcacg aatgatctgc tctgcgtcgc
     1921 tttgaggtaa ctcatctaaa agcgatatga tttttcgttg ttgttctgta agttgcggtt
     1981 ggttgtcatt actggataca gccattttat cgccgagagt ttcttcttcc ataaagaacc
     2041 aatggacggg atgttgtgag agctctgcta atttttccag tttatccatt cttggcatca
     2101 cgcctttcaa ccaaccttgc acggattggg gttttacacc aagacgtctt cccagctctg
     2161 actggtttat attcaattcc tgcaacacct gctgaaggcg ttttacaaag atcatcacca
     2221 cccctcgtaa actagttccg cgatcctaca gaaaaaattg ataagtggca ttacaaatag
     2281 aagttgaaat ttaaatttaa atttgtaatt atcggtgtca tcgtaaagtt cggagggaaa
     2341 catgcaaaaa agtactcaag tgaaaatcct gtcaataatg agccaatcag aattagggcg
     2401 tcgtcttggt aaaacaccgc aaaccataag tgggtggttt aaaaaacgag tgccagcgga
     2461 ggaggttata ccagcatgtg aggcgcttga ctggggagta actccgcatg aattgcgccc
     2521 tgataaatac cctaatccaa ccgatggttt acctgttgag tatcaggcta acgcacaagc
     2581 agcggcggga gttgattcat gaaaatcaag catgagcaca tccgcatggc gatgaatgcg
     2641 tggctgcttt atccgagggt agggcgcaaa aaaatcgctg atgatatagc gacagcatat
     2701 tttgagcttg aaatgactta tccaccaatg catgacacct ctacgacaga gggtattgga
     2761 ttgaacatac aaaatatttt tcgctggctt gaaaaggata cgcctgatgc tgttgaaaaa
     2821 attcaggcac taattccagc tatcctgact gttcttccgc gtgaactgcg ttatcacctc
     2881 agtatttttg acactgttga gcgccgtgca ttactggcgg cccaggaagc gttgagcacg
     2941 gcaattgatg cgcatgatga tgcagtccag gcagtttacc ggaaagcgta tttcagcgac
     3001 ggcgggtcat ccggcgagtc tgttgtggtg cattgatatt tatgccagac ccctgctgat
     3061 tctgttgatt ggggaatcac agaatatcac cagaggatgg ttcgtcacaa gatgaggcaa
     3121 ttatggccgc attaccatac atgcaactgt acatagctga ttacctggct gacaccatgc
     3181 atttgtccgc agaggagcac ggtgcgtatt tgttgctgat gttcaattac tggcaaacag
     3241 ggaaaccaat acctaaaaac aggctggcaa aaatttcccg tctgactaac gagcgatggg
     3301 ctgatgtgga accatccttg cgggagtttt tttgcgataa cagcgatgaa tgggtgcatc
     3361 ttcggattga ggaagatctg gcatcagtca gggaaaaatt gaccaaaaaa tcagccgccg
     3421 gaaaagcatc tgttcaggcc agaagaagca gaaaggaagc atatgttcaa acaaaacaag
     3481 agagagattt aacaggtgtt caaacaaatg ttggtgttgt gtttgaacat gacgcaaaca
     3541 caaaagcaac taataaagat acagatctaa aagaattaaa ccccacacat aacgtgcgtg
     3601 aacgcgagag tattccgacc agtgagtcgc atggtgcgcc gttgcagaca gccgaacctg
     3661 aatacctgga cggcctgagc gaaccgatcg ggaaattttc gatgactact gtctggcagc
     3721 cgtcgccgga ttttcgacaa cgggcagcag tgtggggtat ggctctgcct gagccggaat
     3781 ttacacctgc tgagcttgcc gcattccggg attactggat ggcggagggg aaggttttca
     3841 cgcaggttca gtgggagcag aaatttgccc gccacgtgca gcacgtcagg gcacaggtaa
     3901 aaccagtcag caagggggta agccatgcag catcaggtgg cacggcatca cgggcagttc
     3961 aggaaatccg ggcagcacgc gaacagtggg aacgtgaaaa cggatttatc agcaacggaa
     4021 acggcctgga agctgtggga gcttatgggg gaggtgtatt cgaaccgctg gactcagaag
     4081 aacggggccg caccttcgaa gctctggatt gcccagattg gtgcgatgac tgaacagcaa
     4141 atccgtctgg tctgccgtca gtgcatggac cgctgccggg cgggtgaaac gtggcccccg
     4201 gacctggctg agtttgttgc gctgatttcg gagagtgggg caaatccatt tggtcttacg
     4261 gtggatgccg tgatggaaga gtaccggcgc tggcgcaatg aatcctggcg atacgacggg
     4321 agtgataaat acccgtggcc acagcctgtg ctgtaccaca tctgcctcga aatgcgtacc
     4381 agagggattg agcgccagat gacgcagggt gagttaaaac tacttgcaga acggcaactg
     4441 acgaaatggg caaagcatgt tggtaacggg atgagtgttc cgccagtgcg acgacaactg
     4501 gaaggggcga aacacccgca agggccaacg ccaattgagc ggctgaaaca ggaatacgaa
     4561 cgccggaagg cagctggttt tatttgaatc tgagaaacga ttttgtcgga ggaaatttta
     4621 atggaaaccg tatttgacgc actgaaagca ctgaaaagag cctcttcaca ggtagtggcg
     4681 gcccgccttg gaatcagccg tgaagatgcg gtcaacgaac tgtggaaact gaagcgccgc
     4741 ggtgaagcgg ataacaaggg gtcgatgtgg tggctgattc aggctggtga aagtgaaccg
     4801 gtgtcaccgg taccgaaagt gacagcgcaa atgctgactg aggcgattga acaacatggc
     4861 ccacaaacgg cggatgagct ggcactgatg ttcgggatta cctcccgccg ggcgaattca
     4921 tcgctggcca tggtaatcag caaagggcgt ctgattcgcg tgaatcaggg cggtaaattt
     4981 cgttactgca taccgggcgc tgatttaccg gcagagccgg aagctgcatc cgtagcggaa
     5041 accgatggta aagcctttcc tcagccagca ggtgttgcgt taccagttcg ggaagcagaa
     5101 acacaggaag aaataaaaac ggaaagtgtg gcggtcacag tgcagtcaca gccgtcgttc
     5161 accagaaaac atccggatgg tctgatttta ccatcgctgc atgtggctaa ccgcgagctg
     5221 cgccgggcaa aaggtcaggt tcagaagtgg gagcgagtct gcgccgcgct gcgggagctg
     5281 aacaagcacc gggatattgt tcgacagatt actgattctt cccgccgtgt tgtatcggaa
     5341 aagtgattgc cggaggcgct tatggcaaaa gaatttacac cagaagagcg ggaagaagtg
     5401 aaggcgcgca ttgtggaatt cgtgcgcctg agcggacgag aaacttttcg acaactggca
     5461 gataaaacgg gtgtcagtaa gaccgctatt cgtcgtttat ctggtgcgct tgcggccagt
     5521 ggtgatgtct ggctctctgg ttgcggggta tttccatcag agcaggcgta tcgcgtatgg
     5581 cgtaagacac cggagaaggc tgctgacccg acactgattc gaaagttacc tgacggagaa
     5641 atacgtcgtt acaacagacg gcagaacata atttgtcgtg agtgccgcca gagcgaagtt
     5701 atgcagcgtg tgctggcgtt ctatcgggga aactttcagg aggtgatgga gtgagggtga
     5761 gagtttatat tgccggtcca atgacgggat atgaaaattt caaccgtgag gcgtttcaca
     5821 aggcggaaga ggaactgaaa cgggaagggc ataccgtctt aactccggca gtacttccgg
     5881 acgggctgac acagccgcac tacatggata tttgcatggc aatgattcgt tgtgtggatg
     5941 cgatttacat gctgaatggc tggcagcggt cagcgggcgc taaggcagag ctggcactgg
     6001 cggagaaact ggggcatgcg gtgatttatc aggaggtggc tcgatgagag aggttaacta
     6061 tgaggcgctt cgtgaggcag cacaaaacta tcagtcgacg ctggcgtggt atcaggctac
     6121 cccggacagc ccaaatgctg aacgggattg tgatgcggct cttgctgcgt ttaagcgtca
     6181 tatccgtcat cgggaagcgg atattatcgc tggtttgctg gatggactgg aagaagcaaa
     6241 atcacaactc aacgagcagc gtgagtatta cgaaggcgtt atctctgatg ggagcaagcg
     6301 tattgctgaa ctggatgcgc gggaagttca attaccgact cgctacgacc ttcgatatgg
     6361 acacccgata aatgcagatg agcgacatgt catgatacct aaagaaaatg gcagttggct
     6421 ttacctgatt gacctagaac acgcattacg cgtcgctggc attcgcatca aaggagagga
     6481 gcatggaaat aaaaccagag gatgagttaa gcaatatcgt tttatttccg gtaaaagagg
     6541 atgaccctcg taatcaggtt aattttcttt atgagccatc ggaaagacca tattgtcatc
     6601 acgcctctgt ccgggttgac gaaaaagagc gtcaggtccg ctgtaaaatc tgcggtgcag
     6661 ttgtggagcc atttgactgg atgctctctg tggcgaaaag agaaactagg ctggcagatg
     6721 atgtaaagct attgcgccag gaggaacagg aaaggcggaa aaatatagaa aagttaattc
     6781 agattgagcg taacgcgaaa gcgcggatac gcagggtgac aaaatccaga actgaataaa
     6841 taaatttagc gctgtaaata aaatctaatc cttaactgga ggtatattta tgttaaatac
     6901 ac
//
LOCUS       DAZGLJ010000239         6883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000239 DAZGLJ010000000
VERSION     DAZGLJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.239"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            226..1383
                     /gene="acrE"
                     /locus_tag="ACHK49_004252"
     CDS             226..1383
                     /gene="acrE"
                     /locus_tag="ACHK49_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO9264782.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1395..4499
                     /gene="acrF"
                     /locus_tag="ACHK49_004253"
     CDS             1395..4499
                     /gene="acrF"
                     /locus_tag="ACHK49_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO9264783.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMEFGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            4752..4973
                     /locus_tag="ACHK49_004254"
     CDS             4752..4973
                     /locus_tag="ACHK49_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264784.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            5404..6429
                     /locus_tag="ACHK49_004255"
     CDS             5404..6429
                     /locus_tag="ACHK49_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9264785.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            6497..>6883
                     /locus_tag="ACHK49_004256"
     CDS             6497..>6883
                     /locus_tag="ACHK49_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO9264786.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIE"
BASE COUNT         1673 a         1640 c         1759 g         1811 t
ORIGIN      
        1 aatttacgag gttttaattc tgcctctttc aacccgcgtc aaaataaaac agtagaatat
       61 taatcttttt ttgtgtttat gtgccttgag acgccagtat tcataactat tccttacatc
      121 gacgaatgat aatttgtagg atagcaaact gtatttttct ttctgcgagt taacgcgtta
      181 cctttctggg taaataacgc gcttttggtt ttttgaggaa tagtaatgac gaaacatgcc
      241 aggtttttcc tcctgccctc ctttattctg atctccgcgg ctttaatcgc cggttgtaac
      301 gataagggag aagagaaagc tcacgtcggt gaaccgcagg ttaccgttca tattgtgaaa
      361 acggccccgt tagaagttaa gactgaatta ccaggccgca ccaatgctta ccgtatagcc
      421 gaagttcgcc cacaggttag cgggatcgta ctgaatcgta atttcactga aggcagcgat
      481 gtacaagcag gccagtccct gtaccagatc gatcccgcga cctatcaggc aaattatgac
      541 agcgcgaaag gcgaactggc gaaaagtgaa gccgccgccg ccatcgcgca tttgacggta
      601 aaacgttacg ttccgctcgt gggtacgaaa tacatcagcc agcaggagta cgaccaggcc
      661 attgctgatg ctcgccaggc cgatgccgcc gtgattgccg caaaagccac agtcgaaagc
      721 gctcgcatca atctcgctta taccaaagtg actgcgccaa ttagcggacg tatcggcaaa
      781 tcgaccgtga ccgaaggcgc tcttgtcact aatgggcaaa cgactgaact ggcgactgtc
      841 cagcagctcg atcctatcta cgttgatgtg acccaatcca gcaacgattt tatgaggctg
      901 aagcaatccg tagagcaagg aaatttgcat aaggaaaacg ccaccagcaa cgtagagttg
      961 gtcatggaaa acggtcaaac ctatcccctg aaaggtacgc tgcaattctc tgatgtgacc
     1021 gttgatgaaa gcaccggctc cataacccta cgtgctgtct tccctaaccc gcaacatacg
     1081 cttttgccgg gtatgtttgt gcgtgcacgg attgatgaag gcgtccaacc tgacgccatt
     1141 cttatcccgc aacaaggcgt tagccgcaca ccgcgtggtg atgcaaccgt gctgattgtt
     1201 aacgataaaa gtcaggtgga agcgcgccct gtcgttgcca gtcaggcgat tggcgataaa
     1261 tggttgatta gcgaaggtct gaaatctggc gatcaagtca ttgtcagcgg cctgcaaaaa
     1321 gcgcgtccgg gagagcaggt taaagccact accgataccc ccgcagatac tgcatcgaag
     1381 taaggtaatc tgacatggca aactttttta ttcgacgacc gatatttgca tgggtgctgg
     1441 ccattattct gatgatggcg ggcgcactgg cgatcctaca attgcccgtc gctcagtatc
     1501 caaccattgc accgcctgcg gtttctgttt cggcaaacta tccaggtgct gatgcgcaga
     1561 ccgtgcagga tacggtgacg caggttatcg aacagaatat gaatggtatc gataacctga
     1621 tgtatatgtc ctccaccagc gattccgccg gtagcgtgac aattaccctt accttccagt
     1681 ccgggaccga tcctgatatc gcgcaagtgc aggtgcagaa caaactccag ctcgccacgc
     1741 cgttgctgcc gcaggaggtt cagcagcagg ggatcagtgt tgaaaagtcc agtagcagct
     1801 atttgatggt ggcgggcttt gtctctgata acccagacac cacacaggac gatatctcgg
     1861 actatgtggc ctctaacgtt aaagatacgc ttagccgtct gaatggcgtc ggtgacgtac
     1921 agcttttcgg cgcacagtat gcgatgcgta tctggctgga cgccgatctg ctaaacaaat
     1981 ataaactgac accggttgat gtgattaacc agttgaaggt acagaacgat cagatcgctg
     2041 ccggacagtt gggcggaacg ccagcgttac cagggcaaca gttgaatgcc tcgattattg
     2101 ctcaaacgcg gttaaaaaat ccggaagaat tcggcaaagt gaccctgcgc gtaaacagtg
     2161 acggctcggt ggtacgcctg aaagatgtcg cacgggttga acttggcggt gaaaactata
     2221 acgttatcgc tcgtatcaac ggaaaaccgg cggcgggcct ggggattaag ctggcaaccg
     2281 gcgcgaatgc tctcgatacc gcgaaagcca ttaaggcaaa actggcggaa ttacagccat
     2341 tcttcccgca gggaatgaag gttctctacc cttatgacac cacgccattc gtccagcttt
     2401 ctattcacga agtggtaaaa acgctgttcg aagccattat gctggtgttc ctggtgatgt
     2461 atctgttctt gcagaatatg cgagcaacgc tgatccccac cattgcggta cccgtggtgt
     2521 tgttagggac gtttgccatc ctcgccgctt ttggttactc catcaacaca ctaacgatgt
     2581 tcgggatggt gcttgccatc gggctgctcg tcgatgatgc gatagtggtg gtggagaacg
     2641 tcgagcgcgt gatgatggag gataagctcc cgccaaaaga agcgacggaa aaatcaatgt
     2701 cgcaaatcca gggcgcactg gtgggtatcg cgatggtgct gtcagcggta tttattccga
     2761 tggcattctt cggcggttct actggggcaa tttatcgcca gttctctatc accatcgttt
     2821 cggcaatggc gctttctgtt ctggtggcat tgattcttac ccctgcgtta tgtgcaacgc
     2881 tgcttaaacc cgtctctgct gagcatcacg aaaataaggg cggtttcttc ggctggttta
     2941 atactacctt cgatcatagc gttaaccact acaccaacag cgtcggcaaa atcctcggtt
     3001 ccacaggacg atatttactg atctatgcgc tgattgttgc aggaatggtg gtgttgtttt
     3061 tacgtcttcc gtcttccttc ttacctgaag aggatcaggg tgtctttctg accatgattc
     3121 agttacccgc tggcgcgacg caagagcgga cgcaaaaagt gttggatcaa gtgacggatt
     3181 actatctgaa gaacgagaaa gcgaacgttg aaagtgtctt tacggttaac ggctttagct
     3241 tcagcggcca ggcacaaaac gccggtatgg ccttcgtcag tctgaaaccg tgggaagagc
     3301 gtaatggtga tgaaaacagt gcggaagcgg taatccatcg tgccaaaatg gaatttggca
     3361 agatccgcga cggttttgtc attccattca atatgccagc cattgttgaa ctgggcacgg
     3421 caacgggttt cgactttgag ttaattgatc aggctgggct gggtcacgat gccctaaccc
     3481 aggcccgtaa ccagttgctt ggtatggcgg cgcaacatcc tgccagctta gtcagcgtgc
     3541 gtcctaatgg cctggaagac accgcgcagt ttaaactgga agttgaccag gaaaaggcgc
     3601 aggcattagg tgtttcactt tctgacatca atcagaccat ttcaacggcg ttgggtggga
     3661 cttacgttaa cgacttcatc gaccgtggtc gcgtgaaaaa ggtgtatgtt caggcggatg
     3721 ccaaattccg tatgctgcca gaagatgtcg ataaacttta tgtccgcagc gccaacggcg
     3781 aaatggtgcc attctcggcc tttaccactt cacattgggt gtatggctct ccgcgactgg
     3841 aacgctacaa cggtctgccg tcaatggaga ttcaggggga agccgcgcca ggaaccagtt
     3901 ccggcgatgc catggcgttg atggaaaacc ttgcgtcaaa attacctgct ggcattggtt
     3961 atgactggac gggtatgtcg tatcaggaac gcttatcggg aaaccaggct cccgctctgg
     4021 tagcaatttc ctttgtggtt gttttcctgt gccttgctgc actctatgaa agctggtcaa
     4081 ttcctgtctc ggttatgttg gtcgtgccgt tagggattgt cggcgtgctg ctggcggcga
     4141 cactctttaa tcaaaaaaat gacgtctact ttatggtggg cttgctaacg acaattggct
     4201 tgtcggccaa aaacgctatt ttgattgttg agttcgctaa agatctcatg gagaaagagg
     4261 gtaaaggtgt tgttgaagcg acactaatgg cagtacgtat gcgtctgcgt cctatcctga
     4321 tgacctctct cgcctttatt ctcggcgtat taccgctagc tatcagtaac ggtgccggca
     4381 gtggcgcgca gaacgcagtg ggtatcgggg taatgggcgg aatggtctct gcaacgttgc
     4441 tggcaatctt cttcgtaccg gtgttctttg tggtgatccg ccgttgcttt aaaggataaa
     4501 tcagaaacat aaaggcactt tcgggtgcct ttattatttt cagtgaaacc cataaaaatt
     4561 aataagatat tcttccactc actctttaaa aactttctat agttcccgct cccttcacta
     4621 tttttacaat tcacataatt tgtcattatt cattccgcaa tatttataac ctccggtgat
     4681 aaaatggcat tcagctcgtt aataagagag ttaacttatt aagcgttagc gtttattact
     4741 gaggtaacac catgaaaaga ttaattcctg tggcattgct caccgcattg ctggcgggct
     4801 gtgctcacga ttcgccctgc gttccggttt atgacgatca gggtcgtctg gttcacacca
     4861 atacctgtat gaaaggcacg actcaggata actgggaaac ggctggggct attgccggcg
     4921 gggcagcggc tgttgctggc ctgacgatgg ggatcatcgc tctgtcgaaa taactgaaca
     4981 gggcgcggag tttcgcgctc tgtttaacga tacagtatct ttacataaga taaaatattg
     5041 ctatattctg ctggctaatt ctctgctgat ttagcatgtt atatcgcgtt atttcgctgc
     5101 tttcgaaata acgcatcaat ttaactttca cacttcccac cagaaatata ctcacgctaa
     5161 caatcattat tctgcttagt aaaacacgcg taaatatttt gcgccaaaat gtggcgcatg
     5221 tttcattttc gcaccattgc ggggcgctgt ttttattatt tcctgtctac actcagctta
     5281 ttgcgacgcg aaatcgggcg caaaagctgg cactactttt gcttataaga ggatggccac
     5341 agacaggtaa aagacgtttc ccaaacgtcc tataacgata attttcgcct cacaggaagc
     5401 attatgaaaa agatgatgat agccacactg gctgccgcca gcgtgctgct tgccgttgca
     5461 aatcaggcgc atgctggagc gacgcttgat gccgttcaga aaaaaggttt tgtgcaatgc
     5521 gggatcagtg atggattacc tgggttctct tatgccgatg cagacggtaa gttttcaggt
     5581 attgatgttg atgtttgtcg tggtgttgcc gctgctgtat ttggtgacga cacgaaagtg
     5641 aaatataccc cactcacagc aaaagaacgc ttcacggctt tacagtcagg ggaagtggat
     5701 ttgctctccc gtaatacgac ctggacttca tctcgcgacg ccgggatggg aatggcattt
     5761 actggcgtca cttattacga cggcattggc ttcctgacgc acgataaagc ggggctaaaa
     5821 agcgcgaaag aactggatgg cgctaccgtc tgtattcagg cgggtactga taccgaactc
     5881 aacgttgccg actatttcaa ggcaaacaat atgaagtaca caccggtgac tttcgatcgc
     5941 tctgacgaat cagcgaaggc actggaatct ggtcgctgcg atacgctggc ctcggatcag
     6001 tcgcaactgt atgccctgcg catcaaatta agcaacccag ctgaatggat tgtcttaccg
     6061 gaggttatct ctaaagaacc gcttgggccg gtagttcgtc gtggcgatga tgaatggttc
     6121 tcgattgtac gctggacgct tttcgccatg ctgaatgctg aagagatggg catcaattcc
     6181 cagaacgtcg atgaaaaagc ggctaatcca gcaacgcctg atatggcaca tctgctgggt
     6241 aaagaaggcg attacggcaa ggatctgaag ttggataata aatgggccta taacatcatc
     6301 aaacaggtgg gtaactactc ggaaattttc gagcgtaatg taggttcaga aagcccgctg
     6361 aaaattaaac gtgggcaaaa taatctctgg aataacggcg gtattcagta cgcgccgccc
     6421 gtgcgttaag tcggtgtgag gtaacgagcg tcgctacggc ggcgctctct tcagcgttat
     6481 gctttccgag gtttctatgt ctcatcgccg ctcaaccgtt aaaggctcac tctcctttgc
     6541 caaccctacg gttcgtgcct ggttattcca gatccttgcc gttgttgctg ttgtcggcat
     6601 tgttggttgg ttatttcaca acactgtaac gaatctcagt aatcgtggca ttacttcagg
     6661 ttttgccttt ctggatcgcg gcgctggctt cggtattgtc cagcatttga tcgattacca
     6721 gcagggcgac acctacggac gcgtttttat tgtcggctta ctcaatacgc tactggtttc
     6781 tgcattgtgt attgtgttcg cttctgtttt gggcttcttt atcggtctgg cgagactttc
     6841 ggataactgg ctgctacgaa agctttccac aatttatatt gag
//
LOCUS       DAZGLJ010000240         6829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000240 DAZGLJ010000000
VERSION     DAZGLJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.240"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(274..759)
                     /gene="sixA"
                     /locus_tag="ACHK49_004257"
     CDS             complement(274..759)
                     /gene="sixA"
                     /locus_tag="ACHK49_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO9264787.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(962..3106)
                     /gene="fadJ"
                     /locus_tag="ACHK49_004258"
     CDS             complement(962..3106)
                     /gene="fadJ"
                     /locus_tag="ACHK49_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO9264788.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3106..4416)
                     /gene="fadI"
                     /locus_tag="ACHK49_004259"
     CDS             complement(3106..4416)
                     /gene="fadI"
                     /locus_tag="ACHK49_004259"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO9264789.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4596..4880)
                     /locus_tag="ACHK49_004260"
     CDS             complement(4596..4880)
                     /locus_tag="ACHK49_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO9264790.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFASRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5252..6592
                     /gene="fadL"
                     /locus_tag="ACHK49_004261"
     CDS             5252..6592
                     /gene="fadL"
                     /locus_tag="ACHK49_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO9264791.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1701 a         1829 c         1618 g         1681 t
ORIGIN      
        1 ttcaattaca tcctgcctga ttattcaact ctattttctc tattatctgt attaataaat
       61 gacaatgagt gtcaaacaga cattattcat ttcaacatgt aactttgggc aaaatcaaga
      121 tacgcgcaga agaataattt ccgaatgaaa gtagaaagtg ttttatttat agatggggag
      181 agtcttcgat agtagtgagg ttgacagata tccgctgcct gatgagcaat tatgcccaat
      241 gaatctacct cattgggcat aatttggaac tcatcagata gcttttgcca tcttcagatt
      301 acacgggctc atctgccagt tgaatgtgcc gtttccgctt tcatcaagcg ttacgctggc
      361 aatggccgag gtggtaaaca taggcggcgt ttcgcccgga cataactcgg caaccagata
      421 cccgactaac ggtaagtgag agatcaccag taccgaagca acaccttcat tggtcaacgc
      481 ctgcaaatag gcactgacca gaccaacatc gccgcagggc gttaactccg gcagaacttc
      541 tgcactggaa ggcaggttca gacaatcccc tacttcttcc agtgtttgct cggctcgcag
      601 gaacgggctc accagaacgc gttcgatttc cactttttga cctttcagcc agttcgccat
      661 caggcgagat tcgtcacaac cattagtggt cagaggacga acggaatcac tggcggcatc
      721 gagggctgcg tcgccgtgac gcatgataaa aacttgcata ttgcaccgct tttgttaacc
      781 agattcgccc gttttctttc atctgaaacc agagaaagaa aacggtggcc ggcattgtgc
      841 cttatccatt caccgaatga aacgctgttt tttacctcaa tggcgtaagt atagtcaata
      901 tgcgtttata ttttgaccaa cactccgcca ttcagcgcgg attcatatag ctttgacctt
      961 cttattgcag gtcagttgca gttgttttcc aaaaactttc cccacgcgcg cccatctcga
     1021 ccaaacgctc gcaaggggta aaacgggaac catactgcgt ggcaagtcgt tgcattattg
     1081 caaccacttc gcccgcgccg agagaatcga tatagcggaa cggtccaccg agaaatggcg
     1141 gaaaaccaat gccaaatacc gcaccaatat ccccgtcacg cacgctacgg ataacctgct
     1201 catcaacaca acgtactgct tcattcagca tcaacatcac acaccgttca gcaacctgcg
     1261 gtgcggagag tcgcccctgc ccttgtgcgc caatgagcgg gtaaatggcg ggatcgacct
     1321 gttttttgct tttacgccct ttctgaccat aaagatagaa accccgacca ttttttctgc
     1381 ctttgcgatc gtcgttcaaa attgaagaaa caacatttgc aggcgcgcta aaacgttctc
     1441 cataagcggc ttccagtaca ggaataattt tagtcccggt gtcgattcct acctcatcca
     1501 aaagttggat tggacctacc ggaaaaccaa atttcactag cgcggcatca atgtgctcta
     1561 cccgttcacc ttcggtcagc atgcggatag cttcattaat gtaaggcgct aagatgcgat
     1621 tgacgtaaaa cccggctttg tcacgcacga caatgggcgt tttgccctgt tttttcgcca
     1681 gttttaccgt agtggcgatg gtttgcgccg atgtacccgc atgaggaata atctctacca
     1741 gcggcatttt ttccaccgga ctgaagaaat gcaggccaat aacttgctca ggtcgcgtgg
     1801 cgtgagctgc gatatcacca atcggtaaag atgacgtatt cgaagcaaaa atggtatgag
     1861 cggcgcaatt ttgctccact tccgccacca tctgttgttt taattcgaga ttttcaaaca
     1921 ccgcttcaat aatcagatcg cgatgggcaa agccgcgata gtccgtcgtt ccggagatta
     1981 atgccagctg tttgtcacgt tcgctggctt tgagatgacg acggcgaact ttgccctcca
     2041 gctgatccca actgtacttc agcgcatgat ttatgccctg cgggttgata tctttaattc
     2101 tgaccggaag ccccgcttta caagcagtga cataagcaat accgccgccc atcaagccac
     2161 cacctaaaat tcccacgctg tttaatggcg caggcggtgc atcactaccg ggatctttct
     2221 tcacttccgt actggcaaaa aagatactac gcaaagcttg cgattgtggc gtcatcgcca
     2281 gttcgccaaa cgcccgagct tcggcgtcat aaccgctgct ggtgccctgc gctaatcccg
     2341 tttcaacaac ctccaggatg cgttctgtcg ccggataatt gccttgagtt ttgtgttctg
     2401 ttttcttgcc gaccattttg aacagcagcg cacgacctaa cggccccgcc agaatacgct
     2461 cgcgtacagg tagagggcgg gaagatgggc gatccttctt cgccagctca acagcgactt
     2521 ccagcaaaat ggagtgcgga acaacgtcat ccaccagccc cagctttaat gcctgtttcg
     2581 cccgaagttg ttttccggtg aggatcatct ctaatgctgt gctgacgcct atcagacgcg
     2641 gtaaacgctg ggtgccgcct gaaccgggta acaatccaag ttgtacttca ggcaaaccga
     2701 gcaccgtttt aggatcgtca gtacaaacgc gaccgtggca cgccagcgcc aactccagcc
     2761 caccacccag gcaagcgcca tgaatagccg cgataaccgg aatgggcaaa gcatgaatct
     2821 ccgccatcaa ctgttggccc tgccgcgcca gtgcttccgc ttcttgcgcc gttttgcagt
     2881 tgccgatcat gttgatgtct gcgccagcaa tgaagttgtc cggtttagcg gagacaaaca
     2941 ccacgcctcg caactctttg ttttcacgga gttgcttaat aatggcgcgc acctgcgagg
     3001 caaactccgc cttcagggta ttcattttct cacccggtac gtcgatggtg ataacggcaa
     3061 tgttgtccag acgaacatta agggtaaacg ctgatgtcat ttccattatt ccgcctccag
     3121 aaccattgcc gcgccaagcc caccggcagc acaggcggta actaaaccaa atccaccgcc
     3181 gcgacggcga agttcatgca atgtctgggt aatcatccgt gcgccggtcg ccgcgaaggg
     3241 atgtccgtaa gcaatcgaac cgccaagcac gttaaatttg ctatcgtcca cttcgccagt
     3301 ggcatgtgca cgccccagta cttcacgagc aaaacgttca ctacccagca actgaatatt
     3361 cgccagtgtc tgagctgcaa aggcttcgtg catatcgatc aatgtcagat cgctcattgt
     3421 caaaccggca cgttccagcg ccagcggtgt tgaccaggcc ggaccgagca acatgtcctg
     3481 ccagacatca atcgcagtaa atgcgtagct gcgcagatac cccagcggca ccagccctaa
     3541 ttctttcgcc cgggattcag tcatcaggat caccgctgcc gcgccatcgg tcagcggcgt
     3601 actgtttgcc gccgttaccg ttccgtgttt gcgatcaaac gccgggcgca gctttgcgta
     3661 atcggcaagc gaggaattac cgcgaatatt gttgtcttcg acaagcggtt gtttataagg
     3721 agggataaag gcagtcatca cctcttcttt gagttttccg tctgaccatg cctgagcggc
     3781 acgctgatgc gaacggtgcg ctaatgcatc ttgctgttct cgggtgatgc cgtaggtttt
     3841 cgccatttgt tcggcggtgt cgcccatccg caagccagta gaatattctg ctaccgcagg
     3901 cggtacgggc attaagtcgc gcaaacgcag gcgagagaag agtttcagtc gctggctcat
     3961 ggttcgagct ttgttgacat caaccagcac gcgcgccagt tttttactga cgccaattgg
     4021 caatacggaa gaggaatctg ccccaccggc aatccccgct cggatagttc ccgccatcag
     4081 gctttctgcg acgtttgcaa ccgcctggaa actggtagcg caagcgcggc tgacgctgta
     4141 agcatcggta tgcacattca ttccggtacc gagaacaatt tcacgcgcaa tgttgggggc
     4201 ttcaggcatt tgtacgacct gaccaaagac cagctgttca atcacttcag cggggatctc
     4261 gctgcgtgcc agcagttcgc ctaccaccat cttccctaaa tcaaccgcgg gaatgccatg
     4321 aaaagccgtc gcctggcggg caaaaggcgt acgtaaaccg ctaacaatgg cgatacgatc
     4381 gccctggcgg gtaaccagcg gtaaaacctg acccataaca ctcccctgta aaaaaataaa
     4441 taaagtggtc tgacctgatc atagtcttaa ccattttttt acatttagcc aagcggagaa
     4501 aaaggaaagt gggagctatg acacagtgaa gacaggagaa agaaaagaaa acgcccctgc
     4561 cagcaactga caggggcgta cgtacccgag aggaattaac gcagacccag ctggaagatc
     4621 agcatttcag cttcgcaggc aaaagtgaaa tcgatatcca ggcgcacacc gtcagactct
     4681 tcagtgaaag tcggggtgat tttacacggt tcggattcca cgctacgggc tttttcagtc
     4741 agcgccgcca gcgtttgttc tgcttctgcg cggcttgcaa acacgcggct gtaagacgcg
     4801 gtgcagtcgg agttgtccat aatggtgcca acatccatac agcagcaaac cggggtttca
     4861 tcagcactac atttactcat cgttgatttc ctctgtatgt gcacccaagg tgccagataa
     4921 acgttgtgga tattttacgc ttccggaaag tgctgctcca gttgttaatt ctgcaaaatc
     4981 ggataagtga ccgaaatcac acttaaaaat gatctaaaac aaaattcacc cgaatccgcg
     5041 agtgcgcaac ctccaaattt tgccagctgg atcgcgtttc ttagatcata tttgaaaaaa
     5101 gatggaaaca tacttgcaac attccagctg gtccgaccta tactctcgcc actggtctga
     5161 tttctaagat gtacctcaga ccctacactt cgcgctcctg ttacagcacg taacatagtt
     5221 tgtataaaaa taaatcattg aggttatggt catgagccag aaaaccctgt ttacaaagtc
     5281 tgctctcgca gtcgcagtgg cacttatctc cacccaggcc tggtcggcag gctttcagtt
     5341 aaacgaattt tcttcctctg gcctgggccg ggcttattca ggggaaggcg caattgccga
     5401 tgatgcaggt aacgttagcc gtaaccccgc attgattacc atgtttgacc gcccgacatt
     5461 ttctgcgggt gcggtttata ttgacccgga tgtgaatatc agcggaacgt ctccgtctgg
     5521 tcgtagcctg aaagccgata acatcgcgcc tacggcatgg gttccgaaca tgcactttgt
     5581 tgcaccgatt aacgaccaat ttggttgggg cgcttctatt acctctaact atggtctggc
     5641 aacagagttt aatgatactt atgcaggcgg ctctgtcggg ggtacaactg accttgaaac
     5701 catgaacctg aacttaagcg gtgcgtatcg cttaaataat gcatggagct ttggtcttgg
     5761 tttcaacgcc gtctacgctc gcgcgaaaat tgaacgtttc gcaggcgatc tggggcagtt
     5821 ggttgctggt cagattatgc aatctcctgc cgggcaaact cctcaagggc aagcattggc
     5881 agctaccgcc aacggtatcg acagtaatac caaaatcgct catctgaacg gtaaccagtg
     5941 gggctttggc tggaacgccg gaatcctgta tgaactggat aaaaataacc gctatgcact
     6001 gacctaccgt tctgaagtga aaattgactt caaaggtaac tacagcagcg atcttaatcg
     6061 tgcgtttaat aactacggtt tgccaattcc taccgcgaca ggtggcgcaa cgcaatcggg
     6121 ttatctgacg ctgaacctgc ctgaaatgtg ggaagtgtca ggttataacc gtgttgatcc
     6181 acagtgggcg attcactata gcttggctta caccagctgg agtcagttcc agcagctgaa
     6241 agcgacctca accagtggcg acacgctgtt ccagaaacat gaaggcttta aagatgctta
     6301 ccgcatcgcg ttgggtacca cttattacta cgatgataac tggaccttcc gtaccggtat
     6361 cgcctttgat gacagcccag ttccggctca gaatcgttct atctccattc cggaccagga
     6421 ccgtttctgg ctgagtgcag gtacgactta cgcatttaat aaagatgctt cagtcgacgt
     6481 tggtgtttct tatatgcacg gtcagagcgt gaaaattaac gaaggcccat accagttcga
     6541 gtctgaaggt aaagcctggc tgttcggtac taactttaac tacgcgttct gataacgcgt
     6601 tcgcctggat aaagtcacct gcatagcagg tgactttaac tcccccactt caccgaaagt
     6661 agtgtcctca ttgcttacct cctgagtttt gcaaacagcc tgatggcagc ttgccctttc
     6721 aaatcaataa gcggtatcca cagacaaatc tgaagggaaa ggcatatttt caggcgttct
     6781 gctcgtcctt ctcaaagagt ttacttttct gaatttccag gatactcac
//
LOCUS       DAZGLJ010000241         6801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000241 DAZGLJ010000000
VERSION     DAZGLJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.241"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..611)
                     /locus_tag="ACHK49_004262"
     CDS             complement(<1..611)
                     /locus_tag="ACHK49_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO9264792.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPIPGGLWDHLGARESHHLADSLARQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCF"
     gene            795..1385
                     /gene="espM2"
                     /locus_tag="ACHK49_004263"
     CDS             795..1385
                     /gene="espM2"
                     /locus_tag="ACHK49_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HFO9264793.1"
                     /translation="MPMNTSGTSFSSFGISCHREGSFRNSFRGKNDEVVKCSIGERTI
                     SFSVRKFSRNILDTVNRQSAKDIDGWIKDERIVYPSRVINQEIDNYCFQKNAKISTEE
                     RQRVFSLVSQENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTI
                     ANLLADKFYQKHIDSDIDIVKLRNDIPAYLTRAIQG"
     gene            complement(1408..1493)
                     /locus_tag="ACHK49_004264"
                     /pseudo
     CDS             complement(1408..1493)
                     /locus_tag="ACHK49_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1617..1766
                     /locus_tag="ACHK49_004265"
                     /pseudo
     CDS             1617..1766
                     /locus_tag="ACHK49_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(1999..2862)
                     /gene="potB"
                     /locus_tag="ACHK49_004266"
     CDS             complement(1999..2862)
                     /gene="potB"
                     /locus_tag="ACHK49_004266"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO9264794.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVNELDD"
     gene            complement(2846..3982)
                     /gene="potA"
                     /locus_tag="ACHK49_004267"
     CDS             complement(2846..3982)
                     /gene="potA"
                     /locus_tag="ACHK49_004267"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO9264795.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            4232..5461
                     /gene="pepT"
                     /locus_tag="ACHK49_004268"
     CDS             4232..5461
                     /gene="pepT"
                     /locus_tag="ACHK49_004268"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO9264796.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            complement(5607..6728)
                     /gene="roxA"
                     /locus_tag="ACHK49_004269"
                     /gene_synonym="ycfD"
     CDS             complement(5607..6728)
                     /gene="roxA"
                     /locus_tag="ACHK49_004269"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO9264797.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
BASE COUNT         1858 a         1573 c         1664 g         1706 t
ORIGIN      
        1 taaaaacatt gttttgttat attaaaatga cacttgtctt tgcttataat catttctgga
       61 gcaaaggggt cacggctgag ggggtggaga gcgttacgca ggataagttc agtcaatgct
      121 tcctgatcat ataatgcaca tatttctgaa tttcgagcat ttctgacaaa aacacctgtc
      181 tcaggaatat gtagcgttat cggacaatta agatgctcct cagtacagga aaagtcttgt
      241 aaactaacgg gaaatgcatt cgaatttatt cgatctagta gtgtttttat gacgctttcc
      301 tgcacaagcg gggcagcttg cctctgctcc agatataagc tgtatgcctg cagaaaagtg
      361 ttacctccgt taagttgtct tgccaggctg tctgcgagat gatgagactc tcttgcccca
      421 agatggtccc aaagtccacc aggaataggc tctacactaa aaccatccat aaactggata
      481 taatgaagcc tcatattacg gcctcctata gtaatagtgt cattctgatt gcttcttgca
      541 atatgtcgta aagcctccag ctcattttcc ggcaatacac ttccattaga aaaatttaat
      601 atgactggca tattattatc cttttcaacc cattttgtga actatattgg taacaagagt
      661 caacataccg atcatgagat gggactcaaa caatacaact tttaatagaa tattatttgc
      721 atgcatgttt tttctggcta caataaacac aagcatgcaa tcttgtaaac gcttttttat
      781 aaaaaggaat tataatgccg atgaatactt caggtacgtc tttcagctct tttggtataa
      841 gttgccatag agaaggctcc tttaggaact ctttccgagg gaagaatgat gaggtcgtaa
      901 aatgttcaat tggagaacgg actattagct tttctgttcg caaatttagc agaaatatac
      961 tggatacagt gaacaggcag agtgctaaag atattgatgg gtggataaaa gatgaacgca
     1021 tagtatatcc ctcaagggtg atcaaccagg agattgataa ttactgtttt caaaaaaatg
     1081 caaaaatttc cactgaagag aggcaaagag tcttttctct tgtgagccag gagaatcaac
     1141 taacccttga tgttaaggca gcacaaagtt ctattaatca tgtaataatg gggagtgctt
     1201 cttttggcaa gaaaatggat gctctttgtg atggtatgag ccgggatgta aaaaatcgta
     1261 catcagatac catagcaaac ttgcttgcgg acaagtttta ccagaaacat atcgattcgg
     1321 atattgatat tgtaaaacta cgaaatgata tcccagctta cttgacgcgt gctatacagg
     1381 gatgaggcta gaggagagga ctgcttcagc atcatcggct tcggaccagt aaaagagacg
     1441 cagaaaaatg cacaacaggc accacacgcc atgcatggat tcagattact catggctcac
     1501 cagtacagct ataaatcgta aagaaaacta cagtacgttg tacacagaaa cgtagcgtga
     1561 ttcactattg cacaggtcca tcacaacgac aatattttgt gatgtttgcg tggtgcgccc
     1621 cacttcagga tgctgcagat ctggagatcg taattgagga agagacttca ttgcgggaag
     1681 cctggaaaaa gtatcgggtg ttgttgaatc gtgttgatac atcagcagca cctgatattg
     1741 agtggccgac ttcacctgta gagtaatcaa cggagcaggg atgatatttt gtgcttgaag
     1801 tgcctccccg accattcaga tagtcgggga gggggtattg cgattatacg caatcagttt
     1861 tagttaaaga ccgaagctct cgaaaaatct ttctccaaaa ccagaatgca attgattgat
     1921 tttgatagga tctataacgc cgttacctta aacgacaaaa tcactcaaaa acaaacctaa
     1981 taaattgaat ataaaggatt aatcatctaa ttcgttcacc ttcttattca gcagacgaga
     2041 agcgcgccag taaaccagca acatcaggcc cattacgata gtcagcgtaa tgctggtggc
     2101 tgcaccaaat ggccagtcac gaatattgag gaactgtacc ttgatgacgt taccgatcag
     2161 caggtttttc gcaccgccca tcaggtcgga tacatagaac aggcccatcg ctggcagcat
     2221 caccagcaga catccggcaa taattcccgg catcgtcagt ggaataatga tacggataaa
     2281 agtctgtaac ttgctggcac cgagatcgcg cgccgcctca agcaacggct tatccagttt
     2341 ttcgatactg gagtacagcg gcatcaccat aaacggcagc agaatgtaaa ccagaccgat
     2401 aatcaccgca ctgggcgtga acatgatacg gattggtgtg tcgataacgc ccagccagag
     2461 caaaaactcg ttgagatagc ctttggtgct gaggaaaatt ttcagcccgt agatacgaat
     2521 caatgagttg gtccagaagg gaacaatcag cagaaacagc agcagcggac gcaccttgtg
     2581 tggcagcttc gccagaaacc aggcaaacgg gtagcccagc accaggcagg cgagggtggc
     2641 gatcagcgcc atattcagcg agtgcaatag cacttcaaaa tagagcggat cgagcagacg
     2701 cgtgtagtta tccagcgtaa agaccatttt tacgaaactg gcgtcgtcgc gggtcaaaaa
     2761 gctggtgcca atgatcatca ggttgggcag aaagacaaac aacacaagcc aaccgacaat
     2821 agtgacaatc actacattct ggaacttact tgtgttcttc atcagccagt acgacctccc
     2881 agctttctac ccaattaatg gccatttttt ggtcgagaga gtggtcaaag tcaggatcgt
     2941 cttcattgaa gaattcgctg accatcacca tcttgccatt ttccagttca acaaccgact
     3001 ccagcgtcat gcctttgtag ttacgctcac gaacgtaacc aatcagccct tcagcgtggt
     3061 tgtcgtcgtt aatctcttca acacgtaagt cttccgggcg cagcagaaca tgcagttttt
     3121 gccccggttc aacggcgaag ttaacgtaga tattacattc gcggccttca acgttggcgc
     3181 gtacgcgctg ctcgtctaga cgttcgatga cagtggcgtt aaacatattg atttcgccaa
     3241 tgaagccggc aacaaacagg tttttcggct cttcgtaaat ttcgcgcggt gtaccatctt
     3301 gctcaatacg accctcgcgc atcaccacaa tcctgtctga catggtgagc gcttcttcct
     3361 gatcgtgagt gacaaagacg aaggtaatac caagcttacg ctgtaacgct ttcagctcgt
     3421 tctgcatttg cttacgcagt ttgtaatcca gcgctgagag cgactcatcc agcaacaaca
     3481 gacgaggctt gttaaccacc gcgcgagcaa tggcgacgcg ttgctgttga ccaccagaga
     3541 gctgatgcgg tttacgttga gcgaaggttt ccagttgcac catccgcagg gcttccatca
     3601 cgcggggcgt aatttcagca gcgggggttt tttgcatgcg caacccaaag gccacatttt
     3661 cgaacacggt catgtggggg aaaagtgcgt agctttggaa aacagtgttc acatagcggt
     3721 tttccgccgg aacgtgggtg atgtcctcgt tatccagcat gatgcgtccg gaatcaacag
     3781 tttccagacc tgcaatcagg cgaagaacgg ttgttttacc gcagccagaa gggccaagca
     3841 gcgtgaggaa ctcgccattg ttgatagtca gatccagctg gggaatgacc tctttaccat
     3901 caaagcattt gcgaattccc gccaattgca ccagcggtga aagcgaactc ggttgtttat
     3961 tcaatttttt actctgtccc atgtaaacgc aacggatggc ttaccgatgc ggggtttgtg
     4021 gttaaccacc ttggtgactc ttaatgaggg cggtaattct acggcaaacc gcttgaatcg
     4081 ccaatctttg ttgtgaatta ctggcttagc tttatattca ttaaggtaat gctgataaat
     4141 attcccgctt gcaggggtaa aagtgacctg acgcaatatt tgtcttttct tgcttcttaa
     4201 taatgttgtc acaaaaagtg agggtgacta catggataaa ctacttgagc gatttttgaa
     4261 ctacgtgtct ctggataccc aatcaaaagc aggggtgaga caggttccca gcacggaagg
     4321 ccaatggaag ttattgcatc tgctgaaaga gcagctcgaa gagatggggc ttatcaatgt
     4381 gaccttaagt gagaagggca ctttgatggc gacgttaccg gctaacgtcc ctggcgatat
     4441 cccggcgatt ggctttattt ctcatgtgga tacctcaccg gattgcagcg gcaaaaatgt
     4501 gaatccgcaa attgttgaaa actatcgcgg tggcgatatt gcgctgggta tcggcgatga
     4561 agttttatca ccggttatgt tcccggtgct gcatcagcta ctgggtcaga cgttgattac
     4621 caccgatggt aaaaccttgt taggtgccga tgacaaagca ggtattgcag aaatcatgac
     4681 cgcgctggcg gtattgcaac agaaaaacat tccgcatggt gatattcgcg tcgcctttac
     4741 cccggatgaa gaagtgggca aaggggcgaa acattttgat gttgatgcct tcgatgcccg
     4801 ctgggcttac actgttgacg gtggtggcgt aggcgaactg gagtttgaaa acttcaacgc
     4861 cgcatcggtc aatatcaaaa ttgtcggtaa caatgttcat ccgggcacgg cgaaaggagt
     4921 gatggtaaat gcgctgtcgc tggcggcacg tattcatgcg gaagttccgg cggatgaaag
     4981 cccggaaatg acagaaggct atgaaggttt ctatcacctg gcgagcatga aaggcaccgt
     5041 tgaccgagcc gatatgcact acatcatccg tgatttcgac cgtaaacagt ttgaagcgcg
     5101 taaacgtaaa atgatggaga tcgccaaaaa agtgggcaaa gggttacatc ctgattgcta
     5161 cattgaactg gtgattgaag acagttacta caatatgcgc gagaaagtgg ttgagcatcc
     5221 gcatattatc gatatcgccc agcaggcgat gcgtgactgc gatattgaac cggaactgaa
     5281 accgatccgc ggcggtaccg acggcgcgca gttgtcgttt atgggattac cgtgcccgaa
     5341 cctgttcact ggcggttaca actatcatgg taagcatgag tttgtgactc tggaaggtat
     5401 ggaaaaagcg gtgcaggtga ttgtccgtat tgccgagtta acggcgcaac ggaagtcgtg
     5461 attgcccgat gcgtatgtgg actgccaatt accgggatga aaccgttata ttgatactgg
     5521 tccacatact tatcgtgcct acaggcgttc agtgcatgta ggcacgataa ggcgcaatca
     5581 gcgccgcatc cggcttaaat ccaaacttac ccttcgaaga accaataccc gctattgacc
     5641 agcgccgcga gcatcgcgag gaatgacgga tcttccagcg catcgccaaa attctccgca
     5701 gtcagcgcaa tgttgctggc gagtgcatcc agtgccggac ggtgcgggga atcgatcttc
     5761 tcaccattgg catacacgtc gtcgccaatg cgcaatacgc gcagaccacc caggcgcacc
     5821 agcacttcac cttgtttcag cgcatcgtag atttcatctg gctgataagg cggttccggc
     5881 ggcgcgatat ccagttcatg acgtgactgg gatataaact cgccaaacca ttgcttaaag
     5941 tgttccggct ggttgatcaa ttcgagcatc atctcacgca gtttatccat ctcttgcggc
     6001 agaacgtccg caggatgagc gcgaggtgga acatccggat cgctgtagta gttgccgccc
     6061 agttcacgtt gcagcacata atcggcaaat ccgctaatta attcccgcgt atttggcgcg
     6121 cgaaaaccca cggaatagtt catcgcattt tccagcgcgt agccttcatg cgggaatcct
     6181 ggcggaatat aaagaatatc gccaggctcc agctcttcat cgatgatggc ttcgaacgga
     6241 tcgacctgta acagatccgg gtgcggacag tgctgtttca tttgcagctt ttcgcccact
     6301 cgccagcgac gacgaccggt accctgaatg ataaacacgt cgtactgatc gagatgcggg
     6361 ccgacgccgc cgcccggtac agaaaaagag atcatcagat catcaatacg ccagtccggt
     6421 agttcacgga acggtcgcat cagcgcggcg gtcggctcat gccagtggtt cactgcctgt
     6481 accagtaatg accagttggt ttcaccgaga tgatcgtagc tttcgaacgg gccgtggctg
     6541 acctgccatt tgccatcctg gtgactgacc agtcgactgt caacttcgct ttccatcgcc
     6601 agacccgcca actcgtctgg agagatcggg tcaataaaat tattaaagcc gcgttttaac
     6661 accaccgggc gtttctgcca gtgacgttca agaaaatcgg gccagttaag agtgagttgg
     6721 tattccatgt taagcatcca caggctggta tctgcaaccg attataacgg atgcttaacg
     6781 taatgcgtga agtatggaca t
//
LOCUS       DAZGLJ010000242         6767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000242 DAZGLJ010000000
VERSION     DAZGLJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.242"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(178..1107)
                     /gene="fdhE"
                     /locus_tag="ACHK49_004270"
     CDS             complement(178..1107)
                     /gene="fdhE"
                     /locus_tag="ACHK49_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO9264798.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1104..1739)
                     /gene="fdoI"
                     /locus_tag="ACHK49_004271"
     CDS             complement(1104..1739)
                     /gene="fdoI"
                     /locus_tag="ACHK49_004271"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO9264799.1"
                     /translation="MKRRDTILRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1736..2638)
                     /gene="fdoH"
                     /locus_tag="ACHK49_004272"
     CDS             complement(1736..2638)
                     /gene="fdoH"
                     /locus_tag="ACHK49_004272"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO9264800.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2651..5701)
                     /gene="fdnG"
                     /locus_tag="ACHK49_004273"
     CDS             complement(2651..5701)
                     /gene="fdnG"
                     /locus_tag="ACHK49_004273"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5114..5116),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO9264801.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            5895..6728
                     /gene="fdhD"
                     /locus_tag="ACHK49_004274"
     CDS             5895..6728
                     /gene="fdhD"
                     /locus_tag="ACHK49_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO9264802.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1480 a         1834 c         1834 g         1619 t
ORIGIN      
        1 atacgcttgt catcagtgac atacccataa ccttatcgat gtgttggagg gaagaacgag
       61 gcaaacagac aattgcggca tgatcttgaa tgggtaacgc ggaatacgag agatggtgtc
      121 gtcacaccct atgagctgta atccgggcga ccagcgccac ccggaaaacg tcagagatta
      181 ctccccttca cccggaaaca ggaacgggtt gatggaactg cgggcatagc cttcttgctc
      241 cattcgcgcg tccagtacca gagaggcgag gtcatctgcc acggcttcaa ctttcggttc
      301 tttttcctga taaagaatct tcaggtaagt gccgcagtca tcgcagcttt cggctttaat
      361 cgcggcctgt tcgtcatcca gcgaccagta atgcagtttg ccgctctgct cgcagttgct
      421 gcattttacg cgcactacgt gccattcggt ttcacacagg ttgcagtgca ggtaacgcag
      481 accctgagtg gtgccaattt gcaccatgct ggacaccggc atagaaccac aaaccgggca
      541 atattgacgt tgctcgccgt attcagcgcg ggctttgccg gggatcagat tggccatctg
      601 cgcccagtag agcgacagtg cagcccagat aaacggcgct ttatcgctgc tgacggatga
      661 gaaatcagag gcaaacagtg cgctggccat atcttccagc tcctgagtcg atgccttctc
      721 cagattctca atcactgcca gcgccgggcc gctcatttca ggtttcagct cagcaatcag
      781 cgccatcagc agcttttgcc agtgcttatc acgcggcaga acgtgaatat ccagcggggg
      841 cttgccctgt gcgctggctt ctttaatgcg cgcggtcaga tccatctcca gcggatggtc
      901 gtacagcacc acttcctggg cgtgggcgat aagcgcagca aagcgcaggt aatcacccag
      961 cggattattt tctgccagct cgcgcagacg ctcggcgcgg cggttgtata aattcttgag
     1021 ccgagggaac aataacggcg gaatcatatc cgccgtacgt ttctcgctcg aacccagctc
     1081 atcttgcggg attatgcgaa tactcattca gctttctttt ccgttgtctt gcggacctca
     1141 cggtaccagc gcgggtgatg tttcttcgcc caggcgctgg taacccatcc ttccaccatg
     1201 gcggtaatcg tgcctttcac ccaaagggcg gcgtagatat gcaccatgat aaccacaatt
     1261 aacgccactg cggcaaatga atgcagcatt aacgcgaatc ggatcaccgg gattgagaaa
     1321 gcaggcgcaa aataaggacg ccagataatc acgccgctca ccagcagcag aaccaggaaa
     1381 ataatcgccg cccagaaaac gcatttctga ccgaagttat aacgcccggt gtcacctact
     1441 tcctcgttga cgacgatctt acgaatattc ttcgcccaaa agatatcatc ccgattgatt
     1501 aggttgtgat gccagtaacg gaaaaacatg atgatgaacg aggcaaacat aaccacgccg
     1561 acaaacgggt gcagaattcg cgccagctgc ggtgtgccca tgatttgcat caaccagttg
     1621 aaggacggga acaaaaagcc cagcccgctc accgccgcca ggatgaagca gaaggcggtg
     1681 atccagtggt tgatacgttc cggcgcggtg tagcgcagga tggtgtcacg tcgtttcatt
     1741 tgcgctcctc gtctttctct tcgtgcagat tattctcttc ctcatccgca cggttcggac
     1801 cgacacccac gtagtggaag atactggccg caaaggtagc cgcaaagcca acagccgcga
     1861 gcggtttcca gatgcctttc cagaatttca cggtttcgct gatttccggg ttctccggca
     1921 agccatgata cagatttggc ttgtcagcat ggtgcagcac gtacattacg tgtgtaccac
     1981 cgacgcctgc cggatcgtac agacccgcat tgtcgtaacc gcgggttttc agctcagcaa
     2041 cgcgctcgct cgccagcgtt ttcatcgact ctttcgtacc gaagtgaatc gcgcccgttg
     2101 ggcaggtctt cacgcaggct ggttcttgcc caaccaccac gcggtcaacg cacagcgtac
     2161 atttgtagac gcggttgtct tccgggttga ggcgcggaat gtcgaacgga cagcccgcaa
     2221 tgcaataacc gcagccaatg cactgctcgg actggaagtc gacgataccg ttggcatact
     2281 gaataattgc accttccgcc gggcacgctt tcaggcagcc tggatcggaa cagtgcatac
     2341 agccgtcttt gcggatcagc cattccagtt tgtcgttctg ctccacttcc gagaagcgca
     2401 ttaccgtcca cgatttggcg cttaaatcat tggggttgtc gtacacccca atgttattgc
     2461 cgacggtatc gcgaatgtcg ttccactctg aacacgccac ctgacaggct ttacagccga
     2521 tacaggtggt gacgtcgatg agtttcgcca cttcttcctg gaagtcccgc gcctgaggcg
     2581 cgggggtcag accgttagtc gcggaacgac gaatgatatc ttgagattga taagccatat
     2641 gtcgtctccg ttacaccttt tccacattca caaggaagga cttaaactcc ggcgtctgcg
     2701 tgttcgcatc accaacgaat ggcgttaacg tattggcaat aaagcctttt ttagctacgc
     2761 cttcatagcc ccagtgaatc ggaataccga tggtatcgat atctttgccg ttcgctttca
     2821 gtgtacgaat acgtttggtc accaccgctt tggccttgat atagccacgg ttggaggaga
     2881 ctttcacggt atcgccctgg gcaatgccaa gtttattcgc cagcgactcc ccgatttcca
     2941 caaactgctc tggttgcaaa atcgcgttca acagcgcgtg tttggtccag tagtggaaat
     3001 gctcggtcag acgataggtg gttccgacat acgggaactt atcggcttta cccaatgctt
     3061 cggcgtcgtc tttaaagata cgtgcagccg ggttcgagat aacgtttgga tgcagcgggt
     3121 tagttcccag cggcgtttca aacggctcgt agtgttccgg gaacggacct tccgccatct
     3181 tatcgagggc aaacagacgc cccatgcctt cctgctgcat gataaacggc ccgacgccgc
     3241 tacccggagg cgctgcgctg tagtccggaa tatcccagcc ggtccactta gtgccgtccc
     3301 atttcagcaa ctgacgcttc ggatcccacg ggttaccctg cggatctgcg gaggcgcggt
     3361 tatacagaat gcggcggtta agcggccatg cccatgccca gcccagcgtg ttaccgaggc
     3421 cagacggatc ggcgttatca cgacgcgcca tctggttgcc ttccggcgtc cagctaccgg
     3481 cgaaaatcca gcagccacag gaggttgtac cgtcatcgcg caattgggcg aacgagctaa
     3541 gttgttggcc tttcttgacg ataaccgcac cggttgccgg atcggtaata tcggccagcg
     3601 ccttaccgtt gctctccatc gctacttctt ccgacttcgg ctcatgcgga atggcgtagt
     3661 tccaggtcat gttcagcacc tgatccgggt tcgcgccacc ctgttcggca tacatcttgc
     3721 gcaagcgcag gaagataccg gagaggatct caccgtcagt cagcgcaatc cccggggcgt
     3781 ccgcaccttt ccagtgccac tgcaaccagc gacctgagtt aacgattgaa ccgttctctt
     3841 ctgcgaagca ggtcgatggc agacggaaca cttcggtctg gatcttcgac gagtcaactt
     3901 cgttcagctc accgtggttc tgccagaagt tagaggtttc agtgttcagc gggtcgatgg
     3961 tgacgaggaa cttcagtttc gacagacaac cgatcacttt gtttttgttc gggaatgagg
     4021 caacagggtt aaagccctgg cagatatagc cattgacctt gccttctttc atcatctcga
     4081 agtattgcag gacgtcgtag cctttatccc acttcggcaa ccagtcaaag ccccagctat
     4141 tttccgccgt cgctttatca ccaaagaagg ccttcatcat agagacgaag aatttcgggt
     4201 agttgcccca gtagtttacc tggccttcca gcagcggttt tggcgtgttg gcggtaaggt
     4261 aggtttgcag atcggtctgc ttctcgcttg gcagcgtcat gtaacctggc agactctgcg
     4321 acagtagccc caggtccgtc agcccctgaa tattggagtg accgcgcagg gcgttaacgc
     4381 cgccgccggc catccccata ttgccgagca gcagctggat catcgccatc gtacgaatgt
     4441 tttgcgcacc aacggagtgt tgcgtccagc cgagggcata caggaacgag gcagttttat
     4501 cgtgagcact ggtttctgcg atgtattcgc agactttcag gaacgcgtct tttggcgtac
     4561 cacagatgtt ttcaaccaca tctggcgtgt agcgggaaac gtgctgtttc agcaagttcc
     4621 acacgcagcg cggatgttgc agcgtggtat cgcgtttggc gaagccgttt tcgtccagtt
     4681 cataagtcca ggaggattta tcgtacttgc gtttttccgc gtcgtagccg gtgaacaggc
     4741 catcttcaaa gccgtaatcc tcacgcacta tcaggctggc gttggtatag gcttcggtgt
     4801 attcgcggtt gaatttttca ttgttcagca ggtacagcaa tacgcctgac aggaaagcaa
     4861 tgtcagtacc ggaacgaata ggggcatagt agtcagctac cgccgccgta cgcgtaaagc
     4921 gaggatcgat cacaatcagc ttcgcgccgt tgtgaatttt ggcttccatc gcccagcgga
     4981 acccgaccgg gtgagcttca gcggcgttac cgcccatcac cacgacgagg ttggcgttct
     5041 tgatgtcgac ccagtggttg gtcatcgcac cgcgaccaaa tgttggagca agacttgcta
     5101 ccgttggtcc gtgtcagaca cgcgcctggt tgtcgaccgc gagcataccc agcgcgcggg
     5161 agaatttttg cgttaaatag ccggtttcgt tactcgacgc ggaagcacac agcatcccgg
     5221 tggagagcca gcggttaaca gtcacgcctt cggcgttttg cgcaatgtag ttagcatcgc
     5281 ggtcttcttt catcagtttg gcgatgcgat caaacgcctc ttcccaactg atttgctgcc
     5341 atttatcaga acctggcgca cggtattccg gaaacttcag acggctttcg gagtggatga
     5401 aatccaccag gccagcgcct ttcggacaaa gtgcaccgcg gttgaccggg tgatccggat
     5461 cgccttcgat atggaagata gatgctttgg cgttttttgc tccgtcaccg aggctgtaca
     5521 tcaacagccc acagcctacg gaacaatagg tgcaggtatt acgggtttcg cgggtgcgca
     5581 gcagtttata ctgccgggtt tccgcgagcg ctacgctggg tgcaaaaccc agtgccgctg
     5641 ccgtggtgcc tgccataccg ccagcgcaga tcttaaagaa ctgccttctg ctgacctgca
     5701 tggattgctc cttgtttcga cattgtcacg tcccatttac attcgcttgc tgcgtgtgca
     5761 gggagtggga gttatttttc tttgcggaag gggccgcaaa ggtccagaat tggctcaatt
     5821 tccctccatc caggaagggt ttgtaacaga ataccataat gttggtgtgt gtgttcttat
     5881 ctggttaaga gaaagtgaaa aaaacacagc aaaaagaaat cgaaaatgtg acaaatatca
     5941 caggtgttcg tcaaattgag ttatggcggc gtgatgattt acaacaccca cggttagatg
     6001 aggtcgcgga agaagttccc gttgcgctgg tctacaacgg catttcgcat gtggtgatga
     6061 tggcgtcgcc caaagacctt gagtactttg cgctcggttt ttcgctttcc gaggggataa
     6121 tagaaagtcc gcgcgatatc ttcggcatgg atgtcgttcc ttcctgtaat ggtcttgaag
     6181 tacaaattga gctttccagc cgccgcttta tggggttgaa ggagcgccgc cgggcgctgg
     6241 cgggacgtac gggctgtggc gtatgcggcg tggagcaact taatgacatc ggaaaaccgg
     6301 tgcagccgct accgttcacc cagacgtttg atctcaacaa actggatgat gcattacgtc
     6361 atctcaacga ttttcagcca gtggggcaac tgactggttg tactcacgcc gctgcctgga
     6421 tgttgccatc tggcgaactg gtcggcgggc atgaagacgt gggtcgccat gtggcgctgg
     6481 acaaactgtt aggccgccgg tcacaagaag gggaaagctg gcagcaaggt gcggtactgg
     6541 tttccagtcg tgccagttat gaaatggtgc aaaagtcggc gatgtgcggc gtagagattt
     6601 tgtttgcggt gtctgccgcg accacgcttg ctgtagaagt ggctgagcgt tgtaatctga
     6661 cgctggtggg tttttgtaag ccgggtcggg caacggttta tacccatccg cagcgtttga
     6721 gcaattaatt aaaataagtc ctgcgatata tattaaattg caggact
//
LOCUS       DAZGLJ010000243         6566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000243 DAZGLJ010000000
VERSION     DAZGLJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.243"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1031
                     /locus_tag="ACHK49_004275"
     CDS             <1..1031
                     /locus_tag="ACHK49_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9264803.1"
                     /translation="NLYIRNNGYYCYRDPRTGKEFGLGRDRRIAITEAIQANIELFSG
                     HKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAIRRGLPDAPL
                     EDITTKEIAAMLSGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPVAATRAAKSE
                     VRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGYLYVE
                     QSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTHREPLSSGTVSRYFM
                     RARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRDDRGR
                     EWDKIEIK"
     gene            1166..2449
                     /locus_tag="ACHK49_004276"
     CDS             1166..2449
                     /locus_tag="ACHK49_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO9264804.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(2683..4944)
                     /locus_tag="ACHK49_004277"
     CDS             complement(2683..4944)
                     /locus_tag="ACHK49_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO9264805.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(5127..6560)
                     /locus_tag="ACHK49_004278"
     CDS             complement(5127..6560)
                     /locus_tag="ACHK49_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO9264806.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
BASE COUNT         1762 a         1683 c         1591 g         1530 t
ORIGIN      
        1 ctaaccttta tataagaaac aatggatatt actgctacag ggacccaagg acgggtaaag
       61 agttcggatt aggccgagac aggaggatag caatcactga agcaatacag gccaacattg
      121 agttattttc aggacacaaa cacaagcctc tgacagcgag aatcaacagt gataattctg
      181 ttacgttaca ttcatggctt gatcgctacg aaaaaatcct cgccagcaga ggaatcaagc
      241 agaagactct cataaattac atgagcaaaa ttaaagcaat aaggaggggg ctgcctgatg
      301 ctccacttga agacatcacc acaaaagaaa ttgcggcaat gctcagtgga tacatagacg
      361 agggcaaggc ggcgtcagcc aagttaatca gatcaacact gagcgatgca ttccgagagg
      421 caatagctga aggccatata acaacaaacc cggtcgctgc cactcgcgca gcaaaatcag
      481 aggtaaggag atcaagactt acggctgacg aatacctgaa aatttatcaa gcagcagaat
      541 catcaccatg ttggctaaga cttgcaatgg agctggctgt tgttaccggg cagcgagttg
      601 gtgatttatg cgaaatgaag tggtctgata tcgtagatgg atatctttat gtcgagcaaa
      661 gcaaaacagg cgtaaaaatt gccatcccta caacattgca tgttgatgct ctcgggatat
      721 caatgaagga aacacttgat aaatgcaaag agattcttgg cggagaaacc ataattgcat
      781 ctactcatcg tgaaccgctt tcatccggca cagtatcaag gtattttatg cgcgcacgaa
      841 aagcatcagg tctttccttc gaaggggatc cgccgacctt tcacgagttg cgcagtttgt
      901 ctgcaagact ctatgagaag cagataagcg ataagtttgc tcaacatctt ctcgggcata
      961 agtcggacac catggcatca cagtatcgtg atgacagagg cagggagtgg gacaaaattg
     1021 aaatcaaata atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgataa
     1081 gcaatgcttt tttataatgc caacttagta taaaaaagca ggcttcaacg gattcatttt
     1141 tctatttcat agcccggagc aacctgtgaa cacattttca gtttcccgtc tggcgctggc
     1201 attggctttt ggcgtgacgc tgaccgcctg tagctcaacc ccgcccgatc aacgtccttc
     1261 tgatcaaacc gcgcctggta cctcttctcg cccgattctg tcggcaaaag aagcgcagaa
     1321 tttcgatgct caacactatt ttgcatccct gacaccaggt gcagcagcgt ggaatccttc
     1381 cccgattacc ctgcctgcgc aacctgactt tgttgtcggc ccggcgggca ctcaaggtgt
     1441 aacgcatacc acgattcagg cggcggtaga tgcggcaatt atcaagcgta ccaacaagcg
     1501 ccagtatatt gccgtgatgc ctggtgagta tcagggaacg gtatatgtcc ctgccgctcc
     1561 gggtggaatt actctgtacg gtacaggtga aaaaccgatt gatgtgaaga ttgggctttc
     1621 ccttgatggt ggcatgagcc ctgccgactg gcgtcacgac gtcaacccgc gcggcaaata
     1681 tatgccaggt aaaccagcgt ggtatatgta cgatagctgc cagagcaaac gcagcgacag
     1741 tatcggtgtt ctctgctctg cggtcttctg gtcacaaaac aatggcctgc aactgcaaaa
     1801 tctgaccatc gaaaacacgc tgggcgatag cgtagatgca ggtaaccatc cggcggtggc
     1861 actgcgtact gatggtgacc aggtacagat taacaacgtt aacattctcg gtcgtcagaa
     1921 caccttcttt gtcaccaaca gcggtgtgca gaaccgtctg gaaacgaatc gtcagccgcg
     1981 tacgctggtg accaacagct acattgaagg ggatgtggat atcgtttctg gtcgcggcgc
     2041 agtggtgttc gataacaccg aattccgcgt ggtgaactca cgtactcagc aagaagcgta
     2101 tgtgtttgca ccggctacgc tgtccaacat ttactacggt ttcctcgccg taaacagccg
     2161 tttcaatgct ttcggtgatg gtgtggcgca actgggccgc tcgctggatg ttgatgccaa
     2221 taccaacggt caggtggtga tccgtgatag cgccatcaac gaaggtttta acacggctaa
     2281 accgtgggcc gatgcggtga tctctaatcg tccgtttgcg ggtaataccg gcagcgtaga
     2341 tgataacgac gaaatacagc gcaatctgaa tgacactaac tacaaccgca tgtgggaata
     2401 caataaccgc ggcgtgggta gtaaagtggt tgcagaggcg aagaagtaag agcaattaac
     2461 tatttgccgg atgcggcgta aacgccttat ccggcctacg gttcgatgcg atttgtaggt
     2521 cggataagat gcgcaagcat cgcatccgac aataagtgcc ggatgctgcg aaaatgcctt
     2581 atctggccta cagattcgat gcgattcgta ggtcggataa gatgcgcaag catcgcatcc
     2641 gacaataagt gccgaatgcg acctacattc acatggcgct ttttacatct gacggttttt
     2701 attgaagtta atcaaactac ccgccttgat aatctcgcgc tcttcagcag tcagactttc
     2761 catatagagc gtaatttccg ttaccggcgc atcttcatgg atcacataac ctttaaacgt
     2821 cgtacccgga ttatccagcg ccgctttaat gccagggatg taaatgtaat cccccacttc
     2881 aaaggttggc acttccgcca tctgcagcgg taacatcccc cagttgatga cgttagaacg
     2941 gtagcgttta gtcgcgtact cttcggcaat attcgccaga ccgccaatca cacgctggca
     3001 gctcgccgcc tgttcacgcg cagaaccatc gcctggtttc accgcatata ccatgctgcc
     3061 aatttcagtt tgcagcggat caatatgctc ctgaccagca atctgcttaa tgcgcgcaaa
     3121 cacctctgtc agctcgctga cattccccgc cagacgctga ttttccagct cagcagtcgc
     3181 tttacttctg ccaacataac cgggatcgcg gcgtgacagg gtaaactccg ccagaccaat
     3241 cggatttgaa cgataagaag aggtttcacc ggaaggaatc agttcgtcgg tggtggtcac
     3301 ttcgtcgagg atcttcgagc acactttcag gacgatattg tcagtcagcg cacccaattc
     3361 cggccagtct ttaatgttcg gtccgtaaat cagcggttgc tgagttgccc ctttcacaaa
     3421 gccctgataa acacggtttt tatacggcgt tacatcgaag gcgtactccg gcacgttgtc
     3481 ccagcaatca agttcgctgg cagaggttaa atagccaccg tttgccgcag tcgcagcgat
     3541 agaacgagcg tccatcaacg ccaccgctga catctgccca ttagctggct tagagccttc
     3601 gcggttcggg aagttacgcg tggtgtggcg aatactcaaa ccgttgttga ttggcgtatc
     3661 gcccgcgcca aagcatgggc cgcagaacgc ggttctgatg attgcgcctg cgccaatcaa
     3721 atctgctacc acaccttttt tggcgagatc cataaacacc ggctgtgatg acgggtaaac
     3781 tgccagcgag aaggtgtcat tgccacagga ttgaccgcgc agtgcattcg ccgccgcgat
     3841 gacgttttcg tagttaccgc cagaacagcc cgcgataatc ccctgctgca ctttcaggcg
     3901 accattttcc actttatcca gcagcgagag tttggcttta ccgtgcgcca cgcgttcgga
     3961 ctcaatttca atctcacgca gaatgtcggt caggttctgg ttcagtgtgt cgatttcata
     4021 cacgttgctc gggtggaacg gcagcgcaat cattggtttg atggcgctta aatcaacgct
     4081 gatgcagcca tcgtagtacg ccatcggttg agggttaagc tggcagtaat cctggccgcg
     4141 accgtgcagc gccagccagt tatgcacttc ttcatcagtt tgccagacgg agcttaaaca
     4201 ggtggtttcc gtggtcatca cgtcaacgct gttacggaaa tcggtagaga gcgcgctaac
     4261 gcccggtcca acgaactcca tgactttgtt tttgacgtaa ccgtttttga acaccgcgcc
     4321 aatgatagcc agcgccacat cctgtggccc cacatacggc gcaggttttc cggtcagatg
     4381 caccgcaacc acgcccggat agtcgatatc ccaggtgtca ttaagcagct gttttaccaa
     4441 ctcaccgccg ccctcaccga ctgccattgt ccctaatgca ccgtaacggg tgtggctgtc
     4501 tgacccgagg atcattttgc cgccgcctgc catcatctca cgcatatatt gatggatgac
     4561 cgcaatatgc ggaggcacaa aaataccgcc ataacgctgg gccgccgata aaccaaaaac
     4621 atggtcatca ccgttaatag tgccgccgac ggcgcagagt gagttatggc agttggtcag
     4681 cacatacggc agcgggaaac gttccatacc ggacgcttta gcggtctgta caataccaac
     4741 aaaggtaata tcgtgagagg ctaatgaatc aaacttaatt ttaagtttat ccatatttcc
     4801 ggacgtatta tgcgaagaga gaatagacca ggcaatagtg ccttttttgg cttcttcttt
     4861 tttaatttcg ccggtgaaat gttcttcggc aattatttcg ttattactgg cgagaaacac
     4921 gcctttttca gataacttga tcatcaaatg ctccagatat ctgcgcaccg tgaagtgcgc
     4981 agaaagataa taaatggata tactctaaat aattcgagtt gcaggaaggc gacaagcgag
     5041 tgaatcccca ggagcttaca taagtaagtg actggggtga gcgaacgcag acgcagcaca
     5101 tgcaacttga agtatgacga gaatatttac agcatgttcc agccgatcag catattccac
     5161 caccacacgc cgagggtgat atgcaccagg aaggttaata tcgtcagtac cgcaccgacc
     5221 aaccaccagg atttaatatc gttataaccc acgccaaaga tgaccggacc tgccgcgccg
     5281 ccatagtgag taaccatgcc gccataggag ttggagaaca acagtgccag cgcggttaac
     5341 attaacggtg cgccggagac gttcgccagc atggcaaata ccggtaacat agcaacgata
     5401 taggcactac cggaagcgaa gaaataacgc acgataatgc tgaggaaaat aataacgaag
     5461 aaagcaacgt taccgtgacc atcaaatgcc aggttatttt taaagacttc agctaaccat
     5521 tcgaagaatt taactttcga taataaggag cttaatccga taataccgcc gtaccagatt
     5581 aaggtattcc agccgccttt atttttaacc acgtcttccc aggtaacgat acccagcagc
     5641 agcatggtcg ccataacaac gattgccaca gtggattcat caacccccag agacttactg
     5701 aaaatccagc ccagtagcgc cagcacaaag acaccgagca gcattttttc gcggattttc
     5761 atcggtccca gttcggcaag gcccgcttta gcgatggttt tattatccac cttcttaatt
     5821 tctggcggat acatggtata aatcaccagt ggggtgacca gcagcataat gatgcccggc
     5881 aatccggcgg ccagtgccca tccaccccag ctaatttgca ggtgcagaat gtcgttgatc
     5941 attttcagcg ccagaatgtt ccccgccatt gcggtaaaga acatatagct ggtggttttg
     6001 gtgaccatgt aaatggacat catcaggtaa tgtccgacac gacgcggact tttttccggt
     6061 tcggacccca aagccaccgc cacgctgttg atgatcggta acacaatacc gcccgcacgc
     6121 gcggtgttag acggtgttgc cggagccagt accagatcga ggaataccgt aacgtaaccc
     6181 agacccagcg tggtgttacc gattttacca atcagcagat aggcaatacg tttacctaaa
     6241 ccggtggtca caaatgcggc gcttaaggta aacgccgaga acaccagcca ggtagtacct
     6301 gaagagtaac cgcttaatac ggcggtggtc ttaaacgcac cgtcggataa gttaccgacc
     6361 accaccatgg aggcagcaac ggcaattaac agtacgacag gttccgggaa aggcttgata
     6421 accagcccca caatggccgc caggtaaata ccaaaaagca cccacgccag ttcgcttaat
     6481 cctgccggag ctggcataaa accaataata catgggatcg ctaatatcag aattagcttc
     6541 cataacgatt tcttattcat ttaaaa
//
LOCUS       DAZGLJ010000244         6521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000244 DAZGLJ010000000
VERSION     DAZGLJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.244"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(138..395)
                     /locus_tag="ACHK49_004279"
     CDS             complement(138..395)
                     /locus_tag="ACHK49_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264807.1"
                     /translation="MNNRTTTVSPEQLRRQAQEMLRCAEQMEKTSVAKDTLRKQLTPA
                     LRDLLQAKHRTQKAVDELVDCVAELEGQVSQFETLVKEFTA"
     gene            complement(392..1792)
                     /locus_tag="ACHK49_004280"
     CDS             complement(392..1792)
                     /locus_tag="ACHK49_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO9264808.1"
                     /translation="MTSQNTPAWRNDDLEGAVIGAFFLRGADPEVMDILATLSADVFS
                     ARQYRDIYAGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALR
                     RNAALRDAEKTLTEALQNLRDAHTCEAAEDALKDAQNMMASLSTGKGVIQPVHIDDVL
                     PEVVDRVECRNQGLEKSRALMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDII
                     DKVTEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVARFEDEDWARFSQGVGR
                     MTGRNIWMVDQANLTIDEICATTRHHRMKHPETALVVVDYLGLIKTRSTGRHDLAVGE
                     ISKGLKSLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRD
                     EVYNPETQARGIAEINITKQRNGTLGTIYRRFHNGHFLPVDQESARVLSTPMTPGNPR
                     RYSNNRMSGSKTERLF"
     gene            complement(1789..2667)
                     /locus_tag="ACHK49_004281"
     CDS             complement(1789..2667)
                     /locus_tag="ACHK49_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO9264809.1"
                     /translation="MMTFNLREQQTRLQARMDELRAEIAFAQKGEKPWPYRSCWMREG
                     RGCCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSLKAEELTDNA
                     GIALRFRDCEFDNYLEVNPDAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVSMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADDVIEHYASLDLLIIDEV
                     GVQFGSAAEMAILQEIINARYEGILPTILISNLSPEELWAFISPRIADRITNGGRNWL
                     SFNWPSYRSRIGGVAA"
     gene            complement(2678..3586)
                     /locus_tag="ACHK49_004282"
     CDS             complement(2678..3586)
                     /locus_tag="ACHK49_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO9264810.1"
                     /translation="MRPSDLLLDFGHPVAYYPGLVKYMGSPHAVIFFGQIFYWQDKAH
                     AAEGVHKTREEIQHETGLTFEQQAVARKHLVSRGILVETNKRLEHKMFYRIDCERLNE
                     IINENNQFSRNGETRFRETVKPNFAEEGKPSPRTRETTRRGEGKTNFDLTENTTEITS
                     ENTTESKNTIGASADASAPARSARQEYSPEFEQAWQEYPKRAGGNSKSAAFKAWKARI
                     REGVTPETMLNGVKRYAAWVRVSGNTGTQFVKQASTFFGPDRHFEDFWQQPAAPGGGR
                     QRQVDVLAGLGAMSDKFGKSSDKLTF"
     gene            complement(3573..3806)
                     /locus_tag="ACHK49_004283"
     CDS             complement(3573..3806)
                     /locus_tag="ACHK49_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264811.1"
                     /translation="MIRRVVNSLYHRYNRCPRVGQWFTTSNGHVLRVCLVNTESQMVV
                     CQVQGRTHTLSYPLVAFQSGKMFKRLGGGYASV"
     gene            complement(3803..4465)
                     /locus_tag="ACHK49_004284"
     CDS             complement(3803..4465)
                     /locus_tag="ACHK49_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFO9264812.1"
                     /translation="MIISRPNIDSAISGRYTVSAPYKAGAGGRSPELLEAIYDAPASF
                     LSSMLAHARIMVGWAGEPKGSPVSLSAGTANPVQFTTHEFRSSGGGSFSLLKEAAIMA
                     TVPTSPYLKIEVVNGKAVIFSLHVACHFKRMHQNIVDKIEYLNCSREFFTRNFIPGTY
                     HIYGDSLRGYYITLDGLMMLQLGLSLRTMRYYESCIEAFHEAEISLSHTAFRRNQWEV
                     RP"
     gene            complement(4574..5281)
                     /locus_tag="ACHK49_004285"
     CDS             complement(4574..5281)
                     /locus_tag="ACHK49_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO9264813.1"
                     /translation="MNTAIFNDKASMTSVEIAELVGSQHKDVKRSIERLMGKGVIRSA
                     PMANFEIINNLGLKRNVAAYVFEGEQGKRDSIIVVAQICPEFTARLVDRWRELEEQIR
                     KPMSEIEMVAAMALEAVRQQKRITQVEEKVIHVAETVEQIKKGTIREGYAGYRQLKAK
                     TGLSDDKCCNLVNAYQIPTDTHEFMTPDGLLSRRAIVAVEPFMAAFYRVMEEAEPRGT
                     RWYHPKMGLFQVIGWQR"
     gene            complement(5363..5596)
                     /locus_tag="ACHK49_004286"
     CDS             complement(5363..5596)
                     /locus_tag="ACHK49_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9264814.1"
                     /translation="MLRKLIPQNAIARYIGVTPQAVNLWFSKNSVPSRFVLRVCELVE
                     WKVTPHGLRPDLYPYPEDGIPDSLRKSNGITRD"
     gene            5753..6442
                     /locus_tag="ACHK49_004287"
     CDS             5753..6442
                     /locus_tag="ACHK49_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO9264815.1"
                     /translation="MKNVKSTENRIAMMLKTKGWSQAELARKLGVSAQSVQYWTTGKT
                     FPRSDKLAQLSVISGYPQSWFLGEDASSTFSSAEKHHTREDSVVFNVLDVEFSCGDGT
                     HVRGDLIDVVRSIELDPEYARRLVGNRAFKNIEIGNARGDSMAPTISPGDLLFLDKTV
                     TYFDGDGIYAFCFDGECYVKRLQKIGSKIMVLSDNPNYQPWSIEKEGMALLYIQSKVI
                     SSVPFNINRFG"
BASE COUNT         1609 a         1711 c         1521 g         1680 t
ORIGIN      
        1 ataaacaggg gcatcagctc tgaaccgggt ctgaacaaga cgatccccat acgcggggca
       61 atttcagggg tcagcagtgc tctcacggtc acctcagcga acggtattgc atgaacgcag
      121 gagaaaaaat tcagccatca cgcagtaaac tctttcacca gcgtttcaaa ctggcttacc
      181 tggccttcca gttccgccac gcaatccacc agctcatcca ccgccttttg tgtgcgatgt
      241 tttgcctgca gcagatcacg aagcgccgga gtaagctgct tgcggagcgt atcttttgcc
      301 acgctcgttt tttccatctg ttcagcacaa cgaagcatct cctgcgcctg ccgacgaagt
      361 tgttccggtg aaacagtggt ggttctgttg ttcaaaataa acgctccgtt ttactacccg
      421 acatgcggtt attgctgtat ctgcgcggat tgcccggcgt catgggagtg gaaagaaccc
      481 gggcactctc ctggtccaca ggcagaaaat gcccgttatg aaaacgccgg taaatggtac
      541 ccagcgtgcc attacgctgt ttcgtgatgt tgatttcagc tatgcctctc gcctgtgtct
      601 ccgggttgta cacctcatcc ctgtaaagca tcagaatgat gtctgcatcc gcctctattt
      661 cccctgagtt tttcaggtcc gagttcatgg ggcgtttatt gggtctggat tccacgccac
      721 gggagagctg gctcagagca atcagcggaa aaccgccgga ttttgccagg cttttaagtc
      781 cctttgagat ttcccccaca gcaaggtcgt gacgccccgt gctgcgggtt ttaatcaggc
      841 cgaggtaatc gaccaccacc agcgccgttt ccgggtgttt catccggtgg tgcctcgtgg
      901 ttgcacatat ctcatcaatg gtcaggtttg cctggtccac catccagata ttacgccccg
      961 tcattcgtcc cacgccctgt gagaaacgcg cccagtcttc atcttcaaaa cgggcaacag
     1021 acttaagacg ggataccggc attcccccgg cagcagacac catacgttca ccaatctgaa
     1081 tgttcgccat ctccatggtg aacagaagca cgccatgccc ctgctcagtc accttgtcga
     1141 tgatatccag cgcaagttcg gttttcccca tcgaaggacg ggccgcaatg aataccaggt
     1201 cgcctggctc cataccgccc gtttttgcgt ccagttcatc aataccggtc atcagcgccc
     1261 tggatttctc cagtccctga ttgcggcatt caacacggtc gaccacttcc ggaaggacat
     1321 catcaatgtg aaccggctga atgacgccct ttccggtcga cagtgaggcc atcatgttct
     1381 gcgcatcctt cagggcatcc tcggctgctt cacaggtatg cgcatcccgt aaattctgta
     1441 atgcttcagt cagtgttttt tctgcatcgc gcagtgcggc attacgccgc aacgctgcga
     1501 catagtgctc cagtgaagac ttcacccagg ttttgcgtcc ggtgtcggta atcaccgggg
     1561 caagttccgg catctcattg cacagcagta cggggtcaat gacgccggat acacgtgcct
     1621 gtctgcaaat tcccgcgtaa atatcccggt actgtcgtgc agaaaaaacg tccgccgaca
     1681 atgtggccag aatatccatc acttccggat cagccccacg cagaaaaaac gcgccaatga
     1741 cagcgccttc caggtcatcg ttacgccatg ccggggtgtt ctggctggtc atgcggcaac
     1801 acctccgata cgagaacggt agctgggcca gttaaacgac aaccagttgc gcccgccatt
     1861 ggtgatcctg tcggcaatcc ggggactgat gaacgcccac aattcttccg gtgaaaggtt
     1921 gctgatcaag atagttggca aaataccctc ataccgggca ttgataattt cctgcaaaat
     1981 ggccatttca gccgcactgc caaactgaac gccgacttcg tcgatgatca gcaaatccag
     2041 tgacgcataa tgctcaatga cgtcatccgc tgttttttca ctgtcattcc gccagcagtt
     2101 tttcacagca cgggtaaggc gcatcacgtc ggtgatctcc acactggcca gatagttacg
     2161 gatgatgtgt tttgccattg ataccgccag atgatttttc ccggtaccgc aactgccggt
     2221 cataacaaga ctggtaccgt tctccagcat atctggccag ttctccgcat agcggcgaca
     2281 ggccgcaaga tttctggctg cgtcaggatt aacctccaga taattatcaa actcgcagtc
     2341 ccgaaaacgc agagcaattc cggcgttatc agtcagttct tccgccttga gggacgacag
     2401 ctccatggtc aaatcgttgg cctcagctat caagcagtca gggcagcatg aaattttttc
     2461 tctgtcctcg ccattacgat cgctccacac cagaatatgt gtgtggtatt cgccatgttt
     2521 ttcgcaacac ccgcgccctt cacgcatcca gcaggaacga taaggccatg gtttttcgcc
     2581 cttctgagca aatgcaatct ctgcccgtaa ctcatccatc cgcgcctgta gtcttgtttg
     2641 ttgttcacgc aggttaaacg tcatcatcgc tgtcacctca gaatgtcaat ttgtcactgg
     2701 atttaccgaa tttgtcagac atggctccca ggccagccag gacatcgacc tgtcgctgtc
     2761 gcccacctcc gggagctgct ggctgttgcc agaaatcttc gaagtgacga tcgggtccaa
     2821 agaacgtcga cgcctgcttc acgaactggg tgccggtatt tcctgagaca cgcacccagg
     2881 cagcatagcg tttcacaccg ttgagcatgg tttctggtgt cacaccttcc ctgattcggg
     2941 ctttccaggc tttgaaggct gctgacttgg aattaccacc agcacgtttg ggatattcct
     3001 gccaggcctg ttcaaattcc ggtgaatatt cctgtcgggc agaacgcgct ggtgcagacg
     3061 cgtcagcgga tgcgccaata gtgtttttac tctctgtagt attctctgaa gtaatctctg
     3121 ttgtattctc tgtaagatcg aaattggttt tcccttctcc gcggcgagtg gtttcccgtg
     3181 tccgcggtga aggctttccc tcctccgcga aattgggttt tacagtttcc cgaaaacggg
     3241 tttccccatt tcgggaaaac tgattgtttt cattgataat ttcattaagg cgctcacaat
     3301 ctatacggta gaacattttg tgctcaagac gcttgttggt ttcaaccaaa atgcctctgg
     3361 acacaagatg cttacgcgct acagcctgtt gttcaaatgt aagtccagtt tcgtgttgta
     3421 tctcttcacg cgttttatgt acgccttccg ctgcatgtgc tttatcctgc cagtaaaaaa
     3481 tctgaccaaa gaaaataaca gcgtgcggac ttcccatgta tttaacgagc ccagggtaat
     3541 aagcaaccgg atgcccaaaa tcgagcagaa gatcagacgg acgcatagcc acctcccaga
     3601 cgcttaaaca ttttcccgga ctgaaaagcc accagcggat aactcagggt atgagtacgt
     3661 ccctgaacct ggcagacaac catctggctt tctgtattga ccaggcaaac ccgcagaacg
     3721 tggccgttgc tggtggtgaa ccactgcccc acacgggggc aacggttgta tcggtgatac
     3781 agggaattaa cgacgcggcg aatcatgggc gcacctccca ttgattacgg cggaaagcgg
     3841 tgtgactgag gctgatttct gcctcatgga atgcttcaat gcagctctcg tagtaccgca
     3901 ttgtgcgtag acttaaccca agctgaagca tcatcagacc atcaagggtg atgtaataac
     3961 cacgcaggga gtcaccgtag atgtgataag tacccggtat gaaattgcgg gtaaaaaact
     4021 cgcgtgagca gttcagatac tcgattttgt cgacgatgtt ctggtgcatg cgcttaaagt
     4081 ggcaggcaac atgcagggag aaaataacgg ccttgccgtt gacaacttca attttcaggt
     4141 atggggaagt tgggactgta gccatgatgg cagcctcctt gagcagtgaa aaacttccac
     4201 caccggagct gcgaaactca tgggtggtga actgaacagg gttcgcagta ccggcgctca
     4261 aggaaaccgg cgagcctttc ggctcccctg cccagcccac cataattctg gcgtgcgcga
     4321 gcatggacga taaaaaagac gctggcgcgt cgtatatcgc ctcgagcaat tccgggctgc
     4381 gacccccggc acccgcttta taaggtgcgg agacagtgta acgtcccgaa attgcagaat
     4441 caatatttgg tcttgaaatg atcatatagc tgctgatatc tttagaactg ttcttggatg
     4501 tttcggagcc gttttatgcg aaacagctcc ccgttattga tgttgagtga gccgggttac
     4561 tcccggcttt ttttcaccgc tgccaaccaa taacctgaaa taaccccatt ttcgggtgat
     4621 accagcgagt ccctcgcggt tctgcttcct ccataacccg ataaaaagca gccataaacg
     4681 gttccacagc aacaattgcg cgacgtgaca acaatccgtc cggcgtcatg aactcatgtg
     4741 tgtctgtagg aatttgatag gcgttcacca gattgcagca tttatcatct gacaaaccgg
     4801 tttttgcttt cagttggcga tatccggcat agccctcacg aatagtgccc tttttaattt
     4861 gctcaactgt ttcagcaacg tggatgactt tttcttccac ctgagtgatc cgtttctgtt
     4921 ggcgaacggc ttcaagagcc atcgcggcaa ccatttcgat ttcgctcatt ggcttacgga
     4981 tctgttcttc cagttcacgc cagcggtcta ccaagcgggc ggtgaattca gggcaaatct
     5041 gcgcgacaac aatgatactg tcgcgtttgc cttgttcacc ttcgaatacg taagcagcta
     5101 catttctttt caatcctaag ttgttgatta tttcgaaatt cgccattggc gcacttcgga
     5161 taactccctt ccccatgagt ctttcaatgc tgcgtttaac atctttgtgt tggctgccca
     5221 ccagctctgc aatctcaacg ctggtcatgg atgctttatc gttaaaaatt gcggtgttca
     5281 tctgattgct ccttgaggat gacatttcag aactcacgcc aaaacgtatt catcgtgaat
     5341 tactgcttgt gacagtcagt ctttaatctc tggtaatacc gtttgatttt cgtaacgaat
     5401 caggaattcc atcttcggga taagggtata gatcaggtct taacccatgc ggagtaacct
     5461 tccattcaac caattcacac acccgtaaaa caaaacgaga aggaacggag tttttagaaa
     5521 accacagatt caccgcttgt ggcgtaacac cgatgtatct cgctatggcg ttttgaggga
     5581 tcaatttacg caacatgtcg taatcattca gttttatcac agcgcagctc caacattaac
     5641 tttacaaatc aagaatacat caagaataaa ttaacatgca agtttcaaaa ggatcgaata
     5701 cactaaaatc aagttaatat ttatgtgtat aaagcttcga caggaactca ccatgaagaa
     5761 tgtaaaaagc acagaaaatc ggatagcaat gatgctgaaa acgaaaggct ggagtcaggc
     5821 tgaactagcc cgtaagctgg gtgtaagcgc gcaatcagta caatactgga ctacaggaaa
     5881 gacgtttccc agaagcgata aacttgcaca gctatcagta attagtggtt atccacaatc
     5941 ctggtttttg ggtgaagatg cctcatcgac attctcttca gctgaaaaac accatacaag
     6001 agaagacagc gttgtgttca atgtactgga tgttgaattc agttgcggcg acggaactca
     6061 tgtacgggga gatctaattg atgtagtacg ctcaatagaa cttgatcctg aatatgcccg
     6121 ccgcctggtc ggtaacagag cattcaagaa catagaaatc ggtaacgcca gaggggacag
     6181 catggcccca acaatctccc cgggagactt actgttttta gataaaacag taacttactt
     6241 tgacggcgat ggcatttatg cattttgctt tgatggcgaa tgttacgtca aacgacttca
     6301 aaaaattgga agcaagatca tggtcttatc agacaatccc aactatcagc catggagcat
     6361 cgaaaaagag ggaatggcgc tgctctatat ccagtcaaaa gtaatctcat ctgtaccatt
     6421 caatatcaac agatttggtt agttattgat tttaaattaa attattggtc atccatcaca
     6481 aaaaaatcaa gtttatcaat ttttaattga cagtcgtttt c
//
LOCUS       DAZGLJ010000245         6483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000245 DAZGLJ010000000
VERSION     DAZGLJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.245"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(116..555)
                     /locus_tag="ACHK49_004288"
                     /pseudo
     CDS             complement(116..555)
                     /locus_tag="ACHK49_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1163..4576
                     /locus_tag="ACHK49_004289"
     CDS             1163..4576
                     /locus_tag="ACHK49_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO9264816.1"
                     /translation="MEQSLNFEMLRSQWPELAELACMAERYVHSDPESCLVKLRNYTE
                     LMVRWLYRQERLPEGIKANLYDLMNADVFTSMMPEAIIMKMDALRIHGNRAAHGGRIK
                     AKDTYWLLKEAYLLGIWLYVRYAHGNVDDCPKFTLPPLTQSSGRADEKRLEDAIRAQD
                     ESRERELALQRALQQEQEKAEHLTQRLNEARARNQHVADILSIDEAETRRRLIDSRLL
                     AADWNVGEGLKNTDQVTQEHPVKEQPTATGDGYADYVLWDEAHKPLAVVEAKKTSVNA
                     EQGRIQARLYADWLEKEYDQRPIIFYTNGYDIWLWDDHKTHGYPPRRVFGFYSKESLQ
                     YLIQQRETRLPLNSVPHVKDNEGKAVAGRLYQLETIARVSERFTNKYRQSLIVQATGT
                     GKTRVAIALSKLMIDARWVKRVLFLCDRKELRKQAANAFNQFTNEPLYVVGKSKKADR
                     QNARIYIATYPGMMKIMDHFDVGYFDLIIADESHRSIYNVYGDLFKYFDALQIGLTAT
                     PIDMVSKTTFGLFGCEGRIPTANYSLEDAIADNNLVPYEVVTHTTGFLREGIKREKLS
                     DAQIRELEEQGIDPNTLEFDGKALDEAIYNKDTNRYILRNLMENGLKDRDGQLPGKTI
                     IFARNHKHALLLNELFDDMYPQFAGRFCQVIDNYDPRAEQLIDDFKGLDESTNKELTI
                     AISVDMLDTGIDVPEIVNLVFAKPVKSKVKFWQMIGRGTRLCPGLYGYDDNGKPLDKQ
                     KFRIFDHWGNFEYHELHTEEAEVTATKSLAQKRFEAWVMLGAAAQRKFDKQAVDLVAH
                     QLREQINALDEKSIAVQEKWHQKAQYSDEKVLRQLSPKTQQDLLSVLAPLMQWLDVRG
                     QSDAMRFDMDILAAQTARYTNPEELDVLWPVIVEKVERLPPHLAQVQQQGPRINQLRD
                     LGWWKQASLEELEDIRIHLRGIMHLMEKDATPKFGSIQVDITEDANLIQTENRKTNIR
                     SIDFKLYRQQVQGALEPLFQQNPVLKKIRNGEPVTQSELDELAKLVLIQNPNVDIRAL
                     KEFYPQATASLDKLLRTIIGMDSDAVEVRFAQFAADNSLTSQQLRFLSLLKNHIRDYG
                     TIEMRQLFEQPFTHIHNEGVTGVFPDIEQIARLQKIVEELGVVTDAATV"
     gene            4640..6274
                     /locus_tag="ACHK49_004290"
     CDS             4640..6274
                     /locus_tag="ACHK49_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO9264817.1"
                     /translation="MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQ
                     EQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGSDEMMEVVRDGVFQHFRQLGQAD
                     ASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
                     NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHT
                     EIGTNERGELEEQKIFTGDLLTPWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVEAP
                     DIHYQDTMSQSFSTNFPQASKNAFNLILANPPFTGSLDEEDIDSTLSAMVKTKKTELL
                     FLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLEAVINLPSGVFKPY
                     AGVATAILIFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKHYRTLRG
                     LPVDNFMGEKLASLLKQQYPEGIDAGVDFKDRTQAAFIVPKEDIAAQKYDLSINRYKE
                     VVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML"
     gene            6271..>6483
                     /locus_tag="ACHK49_004291"
     CDS             6271..>6483
                     /locus_tag="ACHK49_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO9264818.1"
                     /translation="MKKNQLVTLGKHIDILSGCAFPSSGFNRNNGVPLIRIRDILSGK
                     TETYYEGSYDLKYLIKKGDLLVGMDGD"
BASE COUNT         1761 a         1577 c         1656 g         1489 t
ORIGIN      
        1 tacgtcattg aacatatcca ggactggccg gcaaaccggg tacgcgatct gttgccctgg
       61 aaagttgatc tgagctctca gtaaatatca atacggttct gacgagccgc ttactcttca
      121 ggcgatttac atgaccttcg acaacaccgt tactccagcg gctgctggtt gcctcacata
      181 tagccgcagc atcagcttcc atccccgcag ccacgcgccg aagttctgct gaaccgtttt
      241 cgtggactcg agtgaaccaa ctgcttagat ttgatttatt tggggttttc agaatacggt
      301 agaactcaag tactagttgc tgcgctattt tcagcgccgg ttctttttca cacatcagac
      361 taataaatcg ggaagcataa ttttcttcac ccctgattat tatccagggc atcaaccagc
      421 ggcttacccg ggacactgaa ggaagtctgg cggcagtagt aactggtggg ttccagcctt
      481 tacgccattt ggcaacagca tccctgacga tggtttcact gcctgtaacc cccgggccac
      541 catttcccgc catatccagt cgctggtgtg ctgaattggt catgtatgaa ggaagatatc
      601 aatggtggta tctccctttc aaaaggaagt gaaaagcttg atgcctgctg ccaggcaatc
      661 aatattacgt gcttttaacg actatcctaa atggtggtag ctatgcagag ctcccggtcc
      721 tagcgctggg agcttatcgc ttatctttac cgtaagcgta aacggaccgc cgtatgtagc
      781 cattaaacca gtattggtaa cgtagatacc caccttttca aaagggaagt ggataaaaga
      841 tagcgaaaat gcgaagagtt gattaatgca ttgaaacaaa attcattaat gatgaacttg
      901 gagaaaagct ccatcgtttt attggctcaa aaaacatatc ccatagatga tcagccatac
      961 cttcattggt tagcaaggta tcctgtgagt agccatggct gaagatgccc attgatgggc
     1021 gaaaaccaag gatctgaccg ttagagcagt tatgtcgtta aacacatcac caccatatgc
     1081 catccggctc acttcgtcgg ccaaattagt gtgatattct ttccagaaat gctgttgtat
     1141 ccacgacata aggcgatgat tgatggagca gtctctcaat tttgaaatgt tgcgcagcca
     1201 gtggccggaa ctggctgaac tcgcgtgtat ggcggaacgc tatgttcact ccgatccgga
     1261 aagttgtctg gtcaagctgc gcaactacac cgaattgatg gtgcgctggt tgtatcgtca
     1321 ggagcggttg ccggaaggta ttaaggctaa tctttacgat ttaatgaatg ctgatgtctt
     1381 taccagcatg atgccggaag ccatcatcat gaaaatggat gcgctgcgta tccatggcaa
     1441 ccgtgccgcg cacggcggac gtatcaaagc taaagatact tactggctgc tcaaagaagc
     1501 gtatttgttg ggaatttggc tgtatgttcg ctacgcccac ggtaatgttg atgactgccc
     1561 caaatttaca ctccctccat taacacaatc ttcaggtcgt gcagatgaaa aacgtctgga
     1621 agatgcaatc agggctcagg atgaaagccg tgagcgcgaa ctggcgctgc aacgcgcact
     1681 acagcaagaa caggaaaagg ccgaacacct cactcaacgc ctgaatgaag ccagagcgcg
     1741 taaccagcat gttgccgata ttctctctat tgatgaagcg gaaacccgcc gtcgcctgat
     1801 tgactctcgc ctgcttgctg ctgactggaa tgtaggcgaa ggccttaaaa ataccgatca
     1861 ggttacgcag gaacatcccg ttaaagaaca acctaccgcg accggagacg gttatgcgga
     1921 ctatgtcttg tgggatgagg cacacaaacc gctggcggtg gtggaagcaa aaaaaaccag
     1981 cgtcaatgcc gagcaaggac gaattcaggc ccggctgtat gcagactggc tggaaaaaga
     2041 atacgaccag cgtccgatca tcttctacac caacggctat gatatctggc tgtgggatga
     2101 ccataaaact catggttatc ccccgcgtcg ggtgtttggc ttctacagca aggaaagcct
     2161 gcaatatctt attcaacagc gtgaaacccg tcttccgctg aacagtgtgc cgcacgtaaa
     2221 agataacgaa ggtaaggccg ttgccggacg tttgtaccag cttgaaacca ttgcccgtgt
     2281 cagcgagcgg tttaccaaca aataccggca atcgctaatt gttcaggcta cgggtacagg
     2341 caaaacccgc gtggcaatag cgctaagcaa actaatgatt gatgcccgct gggtaaaacg
     2401 cgtcctgttt ctttgcgacc gcaaggaact acgtaaacag gcggcgaatg cctttaatca
     2461 attcaccaat gagccgctgt atgtggtcgg gaaatcgaaa aaagcagaca ggcagaatgc
     2521 taggatctac atcgccacct atcctggtat gatgaaaatc atggaccatt tcgatgttgg
     2581 ttatttcgat ttgatcatcg ccgatgaatc ccatcgctct atctacaacg tttacggcga
     2641 tctgtttaag tattttgatg ccctccagat tggcctgacg gccacgccga ttgacatggt
     2701 gagtaaaacc actttcggcc tgtttggttg tgaaggacgt attcctaccg ccaactacag
     2761 cctggaagat gccatcgccg ataacaatct ggttccctat gaggttgtca cgcacaccac
     2821 aggattcctg cgcgaaggca tcaaacggga gaaattaagc gacgcacaaa tccgcgagct
     2881 ggaagaacaa ggcatagatc ccaatacgct ggagtttgat ggcaaggcgc tggatgaggc
     2941 gatctacaac aaagacacca accgctatat cctgcgtaac ctgatggaaa atggcctgaa
     3001 agatcgggac ggccagttgc caggtaaaac catcattttc gcccgtaacc acaaacacgc
     3061 gctgctgttg aatgaattat tcgacgatat gtacccgcag tttgccgggc gcttctgcca
     3121 ggtcatcgac aattacgatc cccgcgccga gcaactgatt gatgatttta aagggctgga
     3181 tgaaagcacc aacaaagagc tgaccatcgc tatctccgtt gacatgctcg ataccggtat
     3241 tgatgtcccg gaaattgtga atctggtctt tgccaaaccg gtcaaatcaa aagtgaagtt
     3301 ctggcagatg attggccgtg gtacgcgcct ctgtccgggg ttgtacggtt acgacgataa
     3361 cggcaaacct ctggataaac agaaattccg cattttcgat cactggggca attttgagta
     3421 tcacgaactg cataccgaag aggccgaagt cacagcgaca aaatcgcttg cacaaaaacg
     3481 cttcgaagcg tgggttatgc tgggggccgc tgcgcaacgt aaattcgaca aacaggcggt
     3541 ggatttagtt gctcaccagc tacgcgagca aatcaatgca ctggatgaaa agtcgattgc
     3601 cgtccaggaa aagtggcatc aaaaagcgca gtacagcgat gaaaaagtgc tgcgccagct
     3661 ttccccgaaa acacagcagg atttgctatc tgtcctggcc ccgctgatgc agtggctgga
     3721 cgtgcgcggg caaagcgacg ctatgcgctt tgatatggat attctggcgg cgcaaactgc
     3781 ccgttatacc aacccggaag agctggatgt gctctggccg gtcatcgtcg aaaaagtgga
     3841 gcgcctgccg ccgcatttgg cgcaggtcca acagcaagga ccgcggatca atcagcttcg
     3901 tgatttaggc tggtggaagc aggccagcct ggaagagctg gaagatatcc gcattcatct
     3961 gcgcggcatc atgcacctga tggaaaaaga cgcgacgccg aaatttggtt cgatacaggt
     4021 ggatattacc gaagatgcga acctgattca gacggaaaac cgcaaaacca acatccgctc
     4081 gattgacttc aaactctatc gccagcaggt gcagggagcg ctggagccgc tgttccagca
     4141 aaatccggtg ctgaagaaaa tccgcaacgg cgagcctgtg acgcaaagcg agctggatga
     4201 actggctaaa ctggtgctga tccagaaccc taacgttgat atccgggcac tgaaagagtt
     4261 ctacccgcag gcgaccgcca gcctggataa actattgcgt accatcatcg ggatggacag
     4321 cgacgcggtg gaagtgcgct ttgctcagtt cgccgctgat aacagcctga ccagccagca
     4381 attgcgcttc ctgtcattgc tgaaaaacca cattcgcgat tacggcacca ttgaaatgcg
     4441 gcagctcttt gaacagcctt ttacgcatat ccacaacgaa ggcgttaccg gtgtgttccc
     4501 tgatatagag cagatcgccc gcctacaaaa gatagtcgaa gagctgggtg ttgtgaccga
     4561 cgcagcaacg gtataatacg cacatgacaa gccccggcag cggggctttt gtatttaaaa
     4621 caggatataa aagctaacaa tgatcactgg tgacttaaaa agtaaaatcg acggcctgtg
     4681 ggaagatttc tgggtgggtg gtatcaccaa cccgctgacc gtaatcgaac agatcaccta
     4741 tctaatgtat tcccggatgc tggatactca ggaacaacgc gacgagaagc gtaaacaaat
     4801 cgcgggtatt gattttaaac cgcgttttgc accagaacag caggagttcc gtttcagtca
     4861 ctatagcaat cttggctcgg atgagatgat ggaagtggtg cgcgatggtg tattccagca
     4921 ttttcgtcag ctcggccagg ctgatgcgtc gaaggtaacg ctgctgggca actttatgaa
     4981 agatgcccgt ctggagattg ttaagccttc attgctgacc aaagcggttg aggtgatcaa
     5041 aaacctgcca ttggatcgcg gcgacaccaa aggcgacctt tacgaatacc tgttaagcaa
     5101 gctgaccact gccggaatca acggccagtt tcgcacaccg cgccacatta tccgcacgat
     5161 ggttgaaatg atggagccga accccgcccg cggcgagacg atttgcgatc cggcctgcgg
     5221 caccggtggc tttctggcaa ccagctatga atatctactg gagaagtaca gctcgctgga
     5281 atccattcat accgagattg gcaccaacga acgtggtgag ctggaagagc aaaaaatctt
     5341 taccggcgat ctgttgaccc cgtggcgtaa ccatgtggat aacaatatgt tccacggtta
     5401 cgactttgac accacaatgt tacgtatcgc cgccatgaac ctgattatgc acggcgtgga
     5461 agcgccggat atccactatc aggacaccat gagccagagc ttcagcacaa acttcccgca
     5521 ggccagtaaa aacgctttca acctgattct ggcaaacccg ccgtttaccg gttcactgga
     5581 cgaagaagat atcgactcca cgctgtcggc aatggtgaaa accaaaaaaa ccgaactact
     5641 gttcctggcg cgtattctgc aaatgctgaa agtgggcggg cgcagtgcca ctatcgtgcc
     5701 gcagggcgtg ctgtttggct ccagtaaggc gcaccagtca cttcgcaaaa cgctggtgga
     5761 agataaccaa ctggaagcgg tgatcaacct gccttccggc gtatttaaac cttacgctgg
     5821 cgtggcgacg gcgatcctga tctttaccaa aggcggtcag acggacgagg tctggttcta
     5881 cgatctgcaa aatgatggct acagcctgga tgacaagcgt aacccaatta aagacaacga
     5941 cctgccgcat ctgctggcaa gctggaagca ttaccgtact ttacgcgggc tgccggttga
     6001 taactttatg ggtgagaagt tagcctcgtt gctgaaacag cagtacccgg aagggattga
     6061 tgctggcgtt gattttaaag atcgcactca ggcggcgttt atagtgccaa aagaagatat
     6121 tgctgcccag aaatacgatc tctctattaa tcgttataaa gaagtggtgt atcaggctga
     6181 ggagtatgaa gatccgaagg tgatattgaa gcggttaaaa gatctggaaa aagagattct
     6241 ggcggatctg gatgagctgg aggggatgct gtgaaaaaaa atcaattggt cacacttggc
     6301 aaacatatag acatattgtc cgggtgtgca ttcccttcaa gtggattcaa taggaacaat
     6361 ggtgttccac taatccgtat tagagatatt ctttcaggaa aaaccgaaac ttattatgaa
     6421 ggcagttatg acttaaaata tcttattaaa aaaggtgatt tactagttgg gatggatgga
     6481 gat
//
LOCUS       DAZGLJ010000246         6425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000246 DAZGLJ010000000
VERSION     DAZGLJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.246"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            283..1533
                     /gene="frc"
                     /locus_tag="ACHK49_004292"
     CDS             283..1533
                     /gene="frc"
                     /locus_tag="ACHK49_004292"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO9264819.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            1587..3281
                     /gene="oxc"
                     /locus_tag="ACHK49_004293"
     CDS             1587..3281
                     /gene="oxc"
                     /locus_tag="ACHK49_004293"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO9264820.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            3351..4295
                     /gene="yfdV"
                     /locus_tag="ACHK49_004294"
     CDS             3351..4295
                     /gene="yfdV"
                     /locus_tag="ACHK49_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO9264821.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            4369..5514
                     /gene="yfdE"
                     /locus_tag="ACHK49_004295"
     CDS             4369..5514
                     /gene="yfdE"
                     /locus_tag="ACHK49_004295"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO9264822.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5570..>6425)
                     /locus_tag="ACHK49_004296"
     CDS             complement(5570..>6425)
                     /locus_tag="ACHK49_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFO9264823.1"
                     /translation="MQGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPE
                     KLSILIADDHPTNRLLLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNM
                     DGFELTRKLREQNSSLPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQL
                     HQVAHIAPQYRHLDIEALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRT
                     FHQCIHRIHGAANILNLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEI
                     AVFCQKND"
BASE COUNT         1697 a         1360 c         1530 g         1838 t
ORIGIN      
        1 aaatcaccgg cgcttcttac agttgtaaga ataacatcac aaatgtcgcg actctctctg
       61 attaccacga atcaatattt atcatattct tcttttcgtc ctcactactt accatgcaga
      121 aaggaattgg aagtggcgat gaaatgattt tcagcacttg atacaggatt ttaaaaatat
      181 cgagtgcagc tacgacgata ttttgcgggt tatttataga agatattacg ccttaaataa
      241 acgctaaatt aacacatatt taattaacta ataggtattg ctatgtcaac tccacttcaa
      301 ggaattaaag ttctcgattt caccggtgtg caatctggcc catcttgtac tcaaatgctg
      361 gcctggtttg gcgctgacgt tattaaaatt gaacgtcccg gcgttggtga cgtaacgcgt
      421 caccagctgc gagatattcc tgatatcgat gcgctttact tcaccatgct taacagtaac
      481 aaacgttcta ttgagttaaa taccaaaaca gcggaaggca aagaggtaat ggaaaagctg
      541 atccgcgaag ctgatatctt agtcgagaac tttcatccag gggccattga tcacatgggc
      601 ttcacctggg agcatattca agaaatcaat ccacgtctga tttttggttc gatcaaaggg
      661 tttgatgagt gttcgcctta tgtgaatgta aaagcctatg aaaacgttgc tcaggcagcg
      721 ggtggcgcgg catccactac gggtttttgg gatggtccgc cgctggtaag cgctgcagcg
      781 ttgggtgaca gcaacaccgg aatgcatttg ctgatcggtt tacttgctgc tttgctgcat
      841 cgcgaaaaaa cggggcgtgg gcaacgagtc accatgtcaa tgcaggatgc cgtattgaac
      901 ctttgccgcg tgaaattacg tgaccagcag cgtctcgata aattgggtta tctggaagaa
      961 tacccgcagt atccgaatgg tacatttggt gatgcagttc cccgcggtgg taatgcaggt
     1021 ggtggcggtc agcctggctg gatcctgaaa tgtaaaggct gggaaaccga tcctaacgcc
     1081 tatatttatt tcactattca ggagcaaaac tgggaaaaca cctgtaaagc catcggcaaa
     1141 ccagaatgga ttaccgatcc ggcatacagt acagcccatg cacgacagcc acatattttc
     1201 gatatttttg ctgaaatcga aaaatacact gtcactattg ataaacatga agcggtggcc
     1261 tatttgactc agtttgatat tccttgtgca ccggttttaa gtatgaaaga aatttcactt
     1321 gatccctctt tgcgccaaag tggcagtgtt gttgaagtgg aacaaccgtt gcgtggaaaa
     1381 tatctgaccg ttggttgtcc aatgaaattc tctgccttta cgccggatat taaagctgcg
     1441 ccgctattag gtgaacatac cgctgctgta ttgcaggagc tgggttatag cgacgatgaa
     1501 attgctgcaa tgaagcaaaa ccacgccatc tgatattaac gggggcttct ggctcccaat
     1561 ttataaaaat ttcgaggtta ttaatcatgt cagatcaact tcaaatgaca gatggtatgc
     1621 atatcatcgt tgaagcatta aaacagaata atattgacac tatttatggt gttgtaggta
     1681 ttcctgtgac ggatatggca cgccatgccc aggcggaagg cattcgttat attggttttc
     1741 gtcatgagca gtcggcaggc tatgccgctg cggcaagcgg ttttcttacc caaaaaccgg
     1801 ggatctgcct gacagtttct gcgccaggat tcctcaatgg tttgaccgca ttggccaacg
     1861 caacggtaaa tggttttccg atgatcatga ttagcggctc cagcgaccgc gcgatcgtcg
     1921 acctacagca aggtgattat gaagagctgg accaaatgaa tgcggcaaaa ccgtatgcca
     1981 aagcagcatt tcgcgttaat cagccgcagg atcttggcat tgcattggca cgcgctatcc
     2041 gggtctctgt atcgggtcgc cctggcggag tttatcttga tttgccagca aatgtcctgg
     2101 ccgcgacgat ggaaaaagac gaagcgttaa ccacgattgt taaagttgaa aatccgtcgc
     2161 cagcattatt gccatgcccg aagtcagtca ctagcgcaat ttcgctttta gcaaaagctg
     2221 aacggccatt aattatcctt ggcaaaggcg cggcgtattc acaagctgat gaacagcttc
     2281 gtgagtttat tgaaagtact cagattccat tcctgccaat gtctatggcg aaagggatcc
     2341 ttgaagatac gcatccactt tctgcggcag ctgcgcgttc gtttgccctg gcaaatgctg
     2401 acgttgtcat gcttgttggt gcacgactga attggttatt ggcacacggt aaaaaaggat
     2461 gggcggcaga tacacagttt attcaactgg atattgaacc gcaggaaatt gacagcaacc
     2521 gccccattgc tgtgccagtc gttggtgata ttgcatccag tatgcaaggt atgctggcag
     2581 aactgaaaca aaacacattt acgactccac tggtatggcg cgatatttta aatatccaca
     2641 agcagcaaaa tgcacaaaaa atgcatgaaa aattaagtac agatactcaa ccattaaatt
     2701 actttaatgc attaagtgct gtgcgcgacg tattgcgcga gaaccaggat atttatttag
     2761 ttaatgaagg tgcaaatacc ctggataatg cacgaaatat tattgatatg tataaaccac
     2821 gtcgtcgtct ggattgtggt acctggggtg tcatgggcat cggtatgggc tatgccatcg
     2881 gtgctagcgt gacttctggt tctccggttg tcgccattga aggtgatagt gcttttggtt
     2941 tcagtgggat ggaaattgaa acgatttgtc gatataacct gccggtgacg atcgttattt
     3001 ttaataatgg cggcatctac agaggagacg gtgttgatct cagtggcgct ggtgcaccat
     3061 caccaacgga tctgttgcac catgcaaggt atgacaaatt aatggatgcg tttcgtggcg
     3121 ttggctataa cgtcaccacg acagatgaac ttcgtcatgc tttaaccacc ggtattcagt
     3181 cgcgcaaacc gaccattatt aatgtggtca tcgaccctgc agcaggaact gaaagtggcc
     3241 atattaccaa acttaaccca aaacaagtcg ctggtaatta aatactgaca tcgtctcctc
     3301 ttgccaactc attatgcgat ggtgagggga cccttatcgg aaatactatt atgctaacat
     3361 tttttattgg cgatttattg cctattatcg taatcatgct gttgggttat tttagcggca
     3421 gacgagaaac tttttcagaa gatcaagccc gcgcatttaa taaactggta ttaaactacg
     3481 ctcttcctgc ggctctattt gtttctatta cccgggcaaa cagggaaatg atttttgcgg
     3541 acactcgtct gacccttgta tcacttgtgg ttattgtcgg atgtttcttt ttctcctggt
     3601 ttggttgcta caaatttttt aaacgtaccc atgcagaagc agctgtatgt gcattaattg
     3661 cgggttcacc taccattgga ttcctggggt ttgcagttct cgatcctatt tatggtgatt
     3721 ccgtatcaac aggtttagtg gtagcaatta tttctattat tgttaacgca attactattc
     3781 ctattggtct gtatttgctg aatccttcct caggagcgga tggtaagaag aatagtaatc
     3841 tgagcgcatt aatttctgcg gcaaaggagc cagtagtatg ggcacctgtt ctggcaacga
     3901 tcctggtgtt ggttggggta aaaattccgg cagcatggga cccaaccttt aatctgattg
     3961 cgaaggctaa ctcaggggta gcggtattcg ctgcggggtt gactctggct gcacataaat
     4021 tcgagttcag tgccgaaatt gcttataaca ccttcctgaa gctgattctg atgccactgg
     4081 cactgcttct cgttggtatg gcatgtcatt tgaacagcga acatctgcag atgatggtac
     4141 tggcaggcgc attaccgccg gcattctccg gaatcattat tgccagccgg tttaatgtct
     4201 acacccgtac tggtacagcg tcattggctg tgagcgtact gggctttgtc gtcacggctc
     4261 ccttgtggat ttatgtcagt cgactggttt cataattaat catgttattg attttacata
     4321 aatgccgaag tgtcttttca ccttcggctt ttttcaaggc ataagcaaat gacaaataat
     4381 gaaagcaaag ggccgtttga aggcttatta gttatcgata tgacacatgt ccttaatgga
     4441 cctttcggaa ctcaacttct ttgtaatatg ggcgcaaggg taattaaagt tgagccgccg
     4501 ggtcatggtg atgatacccg cacatttggt ccctatgtgg atggacagtc actctattac
     4561 agttttatta atcatggcaa agagagtgtg gttcttgatt taaagaatga tcacgataaa
     4621 agtatattta taaatatgct caaacaagct gatgtattag ctgagaattt tcgcccaggt
     4681 acaatggaaa aactggggtt ttcatgggaa acgcttcaag aaatcaaccc gcgcctcata
     4741 tatgcttcat cgtcaggttt cggacatacc ggtccgctaa aagatgctcc tgcctacgat
     4801 accatcattc aggcaatgag cgggataatg atggaaacag gatatcctga tgctccgcca
     4861 gtgcgcgttg gtacatctct tgcggatcta tgcggcggtg tctatttatt cagcggaata
     4921 gtgagtgcac tttatggccg cgaaaagagc cagagagggg cgcatgtcga tatagcgatg
     4981 tttgatgcca cgctgagttt tctggagcat ggtctgatgg catatatcgc gacagggaag
     5041 tcaccacaac gtctgggaaa tcgccatccc tacatggcac cttttgatgt tttcaatact
     5101 caggataagc cgattacgat ttgttgtggt aatgacaagc ttttttctgc gttatgccag
     5161 gcactggagc ttacggaact ggttaatgat ccccgattta gcagcaatat tttacgcgta
     5221 caaaaccagg ctattcttaa acaatatatt gagcggacgt taaaaacgca ggcagctgaa
     5281 gtttggttag ccagaataca tgaagttggt gtacccgtcg cgccgttatt aagtgtggct
     5341 gaggccatta aattgccaca aactcaggcg agaaatatgt tgattgaagc cgggggaata
     5401 atgatgccgg gtaatccgat aaaaatcagc ggctgcgcgg acccgcatgt tatgccggga
     5461 gcggcaacgc tcgaccagca tggggaacaa attcgccagg agttctcatc ataaatgtaa
     5521 gatgtgctgg taaatagctc ccacatttga acattgtggg agccgctatt tagtcatttt
     5581 tctgacagaa aacagcaatc tcctggtcca gctctgcaat gtgttctttt acagagttca
     5641 gcaactgaag aatttcaggc ttactgtcat ctgaaacagg tgttatttct aactgatggc
     5701 taatattaat caacttttgc aaattcagga tgttagccgc accgtggatg cgatgaatac
     5761 actgatggaa agttctgtta tcgccagctt ctagtgcttg aaacgcagcg ggtagatctt
     5821 tatgcgtttc atgctggaaa gtcatgagaa tctcctgcat cagttgtaga tcgttcgccg
     5881 tattattttt cagggcttcg atatcaaggt ggcgatactg aggtgcaata tgcgcaacct
     5941 ggtgtaactg acttaaatgt gttttcagta catccagggt caacggtttg aacaaacata
     6001 agttcatgcc gcaacttaac cctttttcac gttcgttagc ctgtgcgttg gctgtaagcc
     6061 cccagatggg taaggaagaa ttttgctcac ggagtttgcg agtcaactca aaaccatcca
     6121 tattcggcat attaacgtca gtaataagca gatcataatg ttgcatactg actttgtgta
     6181 gcgcttgcac accatcagtg gcttcatcaa catcatatcc taatagattt agctggcgtt
     6241 tgagtaatag cctgttggtc ggatgatcat ccgcgattaa tatgctcaac ttttcaggta
     6301 gtgtgatggg gtgttctgct tttgcctcga cagtcgccac ttgctggcta atttctaccg
     6361 ggattgtgat cgtaaatgtt gttcctatgc ctggatgact ttctaatgac aaatcgccct
     6421 gcata
//
LOCUS       DAZGLJ010000247         6374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000247 DAZGLJ010000000
VERSION     DAZGLJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.247"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..72)
                     /locus_tag="ACHK49_004297"
     CDS             complement(<1..72)
                     /locus_tag="ACHK49_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264824.1"
                     /translation="MREQIKQDIDLIEILFYLKKKVRV"
     gene            complement(285..1955)
                     /gene="betA"
                     /locus_tag="ACHK49_004298"
     CDS             complement(285..1955)
                     /gene="betA"
                     /locus_tag="ACHK49_004298"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO9264825.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRI
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1969..3441)
                     /gene="betB"
                     /locus_tag="ACHK49_004299"
     CDS             complement(1969..3441)
                     /gene="betB"
                     /locus_tag="ACHK49_004299"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO9264826.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGGGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFSPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3455..4042)
                     /gene="betI"
                     /locus_tag="ACHK49_004300"
     CDS             complement(3455..4042)
                     /gene="betI"
                     /locus_tag="ACHK49_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO9264827.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4171..6204
                     /gene="betT"
                     /locus_tag="ACHK49_004301"
     CDS             4171..6204
                     /gene="betT"
                     /locus_tag="ACHK49_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO9264828.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1315 a         1746 c         1744 g         1569 t
ORIGIN      
        1 aacacgaacc tttttcttca gataaaataa aatctcaatc agatcgatat cctgtttgat
       61 ttgttcacgc ataatatatc cagaaaataa aatctgtcgc agataaggtt gtattaatag
      121 tctgtatcag gaatgttcgg gttaaatatc agcaaaaagc cagcatcatg gatactggat
      181 atgaagcatg agagttacct cggtgtttat ataaggattc ggtccccctc tctggaacgg
      241 taactctcaa tctgatcggt tcctgcgtta gttcacatca cgactcattt tttcgctctc
      301 accggcatcc catttgccac aaaatatccc gccgtgctcc tcggcagcgc ttcctgtcca
      361 cgaatcatat ccgctatttt ctcgccaatc ataattgtcg tggcgttcaa attcccggtg
      421 ataatctgcg gcataatcga cgcatccacc acacgcaggc cttctaaccc gtgtacgcgg
      481 ccttcgccgt caaccacgga catctcgtcg taacccattt tgcaggtacc gcacggatgg
      541 aaggcggttt cggcgtggtt acgcacgaac tcatcgagct gttcatccgt ctgacattcc
      601 gtgccggggc tgatttcgcg gccacgatac tgatccagcg cgggttgatg catgatctcg
      661 cgggtgatgc gaattgcgtc gcggaactcc tgccagtcct gctcgtgcga catgtagtta
      721 aacagaatcg ccggatgctg gtgcgggtcg cgggatttaa tccgcacatg cccacggctt
      781 ggcgagcgca ttgagccgac gtggcactgg aaaccgtgct ctttcactgc attcgagccg
      841 ttatagttaa tcgctactgg caggaaatgg tactgaatat tcggccacgc aaattcctca
      901 cggctgcgaa taaatccacc tgcttcaaag tggttgctgg caccaacgcc agtgccgcca
      961 aacagccact ccgcaccgat tttcggctgg ttccaccact gcagggcagg gtagagggaa
     1021 accggttctt tgcactcata ttgcagatac atctccagat gatcctgaag attttcgccg
     1081 acgccgggta attcatgcac cagcggaata tcaaactccg ccagcagttc agcgttgccg
     1141 acgccggagc gttgcaggat ctgcggtgag gcaatcgcgc ctgcacataa cagcacttct
     1201 ttgttggccg ttgcgcgggt tgggatggtg ctgtcgcctt ccagccattc gacgcccacc
     1261 gcgcgtttgc cgtcaaaaat gatgtgatcg gtcatagcgt gaatacgaat ggtcaggtta
     1321 gggcgcgatt tggcctgatc gagataaccg cgcgcggtac tggcgcgacg gccctgcggc
     1381 gtgacggtgc gatccatcgg accaaaacct tcctgctgat aaccgttgag atcgtccgtg
     1441 cgcgggtagc ccgcctgcac gcccgcttca atcatcgctt caaacagcgg attgacgccg
     1501 ggtttggagg tagtgacgct caccgggcca tcgccaccgt ggtagtcgtt ctcgcccaca
     1561 tcgcgagtct cggccttgcg gtagtagggc aggcagtcga gataactcca gttctccaga
     1621 ccgggttctt gcgcccagtt atcgagatcc agcgcattgc cacggatgta gcacatgccg
     1681 ttgatcagcg acgatccacc caggccttta ccgcgtccgc actccatgcg gcggttattc
     1741 ataaacggtt caggctctgt ttcataggcc cagttgtagc gtttgccctg tagtgggaac
     1801 gccagcgctg cgggcatctg ggtgcggaag tcaaagcgat agtccgggcc gcccgcttca
     1861 agcagcagca cggaggtatt cggatcttca gtcagacggg tagcgagaac gttgccggct
     1921 gagccggcac caataatgat gtagtcaaat tgcaaataaa cctcctggtt agaatatgga
     1981 ctggaattta gccatctcaa cctggatgga cttcacctgg gtgtaactct ggagcgtcat
     2041 cacgccgttc tcgcgaccaa tgccggagtg tttgtagccg ccaacgggca tctctgccgg
     2101 ggattcgccc caggtgttga tccagcaaat acccgcttcc agctgatgaa tgacgcgatg
     2161 cgcgcggttc aggtccgccg tcacgatgcc cgccgccagg ccgtagtcgg tatcattggc
     2221 gcggcgaatc acttcgtcct cagattcata agtgagaagc gacatcaccg gaccgaagat
     2281 ctcttcgcgc acgatggtca tttcgtcgcg gcaatcggtg aacacggtcg gtgcaaccca
     2341 tgcgccgtta tcgaagccat cgcctttcag tacatcgccg ccgcacagta cgcgcgcgcc
     2401 ttcctctttg cctttggcga tatagcgcag cacgttatcg cgatgcggga agctgaccag
     2461 cgggctgaag ttagtttgcg gatcgaaaac gtcgcccgcg cgaatgcgct caacgcgcgc
     2521 cagaattttc tgctcaaatg cggctttgca tttcgccgga acgaagacgc gggtgccgtt
     2581 ggtacacacc tgaccggagc tgaagaagtt cgccatcatg gcgatatcgg cggcgagatc
     2641 gagatccgca tcgtcgaaaa cgatcagcgg tgatttaccg cccagttcca tggtcacttc
     2701 tttcagggaa gaggccgccg agttagccat cacttttttg ccgctggcga caccgccggt
     2761 aaatgacact ttggcaatgc ccggatgctc ggtcagatat tgcccggtct ccgcgccccc
     2821 ccccggcaac acgttaaata cgccgtccgg caggcccgct tcgctgtaaa tttcagccag
     2881 ctttaacgcg gtaagcgggg tgacttcgct cggtttgaaa atcattgcgt tgcctgccgc
     2941 cagcgccggg gcggatttcc acagggcaat ctggatcggg tagttccatg cgccaatccc
     3001 cgccaccaca cccagcggtt cgcggcgggt ataaacaaat gacgtctcac gcaacgggat
     3061 ctggctgcct tccagcgccg ggatcagccc ggcgtagtac tccagcacgt ccgcaccggt
     3121 aacgatatcg acggttgagg tttccgaata tgcttttccg gtatcgaggg tttccagttt
     3181 tgcgagttcg tcattgcgtt cacgcagaat atcaacggcc cgacgcagaa tacgcgagcg
     3241 ctccatagcg gtcatcgccg cccagatttt ttgcccctgt tgggcactct tcacggcgcg
     3301 atcgacatcc tcacgcccgg cggcctgcac ggtcgccagc acgttaccgt tggccgggtt
     3361 aatggtctcg aaggtgcgac cgctggtggc ggaggtataa ccaccatgta tataaagctg
     3421 ctgttctgcc attcgggaca tcacgtctcc tcggttaatc ggtgggtaaa tgctgagtaa
     3481 taaagtgacg ggtcagcgag tgtgcgagag gtttatccag cggtttgccg ctcagtgccg
     3541 cgcgcagcca taatccgtca atcagcgcgg caaggccgta gcccgcttcc tgtgcctgtt
     3601 ggcgcggtaa ctcgcggcga aattcgctca ccagattcga caataagcgg cggctactga
     3661 cctgctgtaa acgatagagc atcggctgat gcatactgct ggcccagaac gccagccagg
     3721 ctttcatcgc cgcactgctc acctgcgttt catcgaagtt tccgccaaca atcgcctgta
     3781 atcgctgctc tgcactgccc tgcggaagtg catgtaatcg attcaaaacc gcgtcacgca
     3841 gctgactggt gatatcgcgc atggttgctt ccagcagacc atttttgtcc ctgaaatagt
     3901 ggctgatgat ccccgtagaa acgcctgcac ggcgggcgat ctgcgcgatc gttgcatcgt
     3961 gcatgcccac ttcatttatt gcttccagtg tggcgtcgat cagttgtctg cgccggatcg
     4021 actgcatccc caatttgggc attttcgcca ctccattcat cagcgttgtt tatctattaa
     4081 agcggttatt gattggacgt tcaatataaa atgtgtctta attgttacga atttgatttt
     4141 aaatggtaac aataacagtg gggatactgg atgacagacc tttcacacag cagggaaaag
     4201 gacaaaatca atccggtggt gttttacacc tccgccggac tgattttgtt gttttccctg
     4261 acaacgatcc tgtttcgcga cttctcggcc ctgtggattg gccgcacgct ggactgggtt
     4321 tctaaaacct tcggttggta ctatctactg gcggcaacgc tctatattgt ctttgtggtc
     4381 tgtatcgctt gttcgcgttt tggttcggtg aagctcgggc cagaacaatc caaaccggaa
     4441 ttcagcctgc tgagttgggc ggcgatgctg tttgctgccg ggatcggtat cgacctgatg
     4501 ttcttctccg tagccgaacc ggtaacgcag tatatgcagc cgccggaagg cgcgggacag
     4561 acgattgagg ccgcgcgtca ggcgatggtc tggacgctgt ttcactacgg cttaaccggc
     4621 tggtcgatgt atgcgctgat gggcatggcg ctcggatact ttagctatcg ttataatttg
     4681 ccgctcacca tccgctcggc gctgtacccg atcttcggta aacggattaa cgggccgata
     4741 gggcactcag tggatattgc agcggtgatc ggcaccatct tcggtattgc cactacgctc
     4801 ggtatcggtg tggtgcagct taactatggc ttgagcgtac tgtttgatat tcccgattcg
     4861 atggcggcaa aagcggcact gatcgccttg tcggtgataa tcgccacgat ctctgtcacc
     4921 tccggtgtcg ataaggggat tcgtgtgtta tcggaactta acgttgcgct ggcgctggga
     4981 ttgatcctgt tcgtattgtt tatgggcgac acctcgttcc tgcttaatgc gctggtgctg
     5041 aatgttggcg actatgtgaa tcgctttatg ggcatgacgc tcaacagttt tgccttcgac
     5101 cgtccagttg agtggatgaa taactggacg ctcttcttct gggcatggtg ggtggcatgg
     5161 tcgccgtttg tcggcttgtt cctggcgcgt atctcgcgtg ggcgtaccat tcgccagttc
     5221 gtgctgggca cgttgattat tccgtttacc ttcacgctgt tatggctctc ggtgttcggc
     5281 aatagcgcgc tgtatgaaat catccacggc ggcgcggcat ttgccgagga agcgatggtc
     5341 catccggagc gcggcttcta cagcctgctg gcgcagtatc cggcgtttac ctttagcgcc
     5401 tccgtcgcca ccattactgg cctgctgttt tatgtgacct cggcggactc cggtgcgctg
     5461 gtgctgggga atttcacctc gcagcttaaa gatatcaaca gcgacgcccc cggctggctg
     5521 cgcgtcttct ggtcggtggc gattggcctg ctgacgctcg gcatgctgat gaccaacggg
     5581 atatccgcgc tgcaaaacac cacggtgatt atggggctgc cgttcagctt tgtgatcttc
     5641 ttcgtgatgg cggggttgta taaatctctg aaggtagaag attaccgccg tgaaagtgcc
     5701 aaccgcgata ccgcaccgcg accgctgggg cttcaggatc gcctgagctg gaaaaaacgt
     5761 ctctcgcgcc tgatgaatta tccgggcacg cgttacacta aacagatgat ggagacggtc
     5821 tgttacccgg caatggaaga agtggcgcag gagctgcggt tgcgcggcgc atacgtggag
     5881 ctaaaaagcc tgccgccgga agagggacaa cagttggggc atctggattt gttggtgcat
     5941 atgggcgaag aacaaaactt tgtctatcag atttggccgc agcaatattc ggtgccgggc
     6001 tttacctacc gcgcacgtag cggtaaatcg acctactacc ggctggaaac cttcctgtta
     6061 gaaggcagcc agggcaacga cctgatggac tacagcaaag agcaggtgat caccgatatt
     6121 cttgaccagt acgagcggca ccttaacttt attcatctcc atcgtgaagc gccgggccat
     6181 agcgtgatgt tcccggacgc gtgattgttt ttgcataaaa ccgcgccatg tctgcatatg
     6241 gcgcgggaat cattgactcg ctgtaaaaaa ttatgcatat attatatatg attgtcatgt
     6301 catatcctgc aattcctctt gtatcacttc atgattttat attttattaa ttaacgattt
     6361 tattttgtat tagc
//
LOCUS       DAZGLJ010000248         6310 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000248 DAZGLJ010000000
VERSION     DAZGLJ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.248"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..338
                     /gene="ydjO"
                     /locus_tag="ACHK49_004302"
     CDS             <1..338
                     /gene="ydjO"
                     /locus_tag="ACHK49_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFO9264829.1"
                     /translation="RFKKNESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDIMSIP
                     DSSLRKRLGYEVLSFSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATA
                     PIQNGPLPA"
     gene            complement(342..1733)
                     /gene="tcyP"
                     /locus_tag="ACHK49_004303"
     CDS             complement(342..1733)
                     /gene="tcyP"
                     /locus_tag="ACHK49_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO9264830.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1866..2456)
                     /gene="ydjM"
                     /locus_tag="ACHK49_004304"
     CDS             complement(1866..2456)
                     /gene="ydjM"
                     /locus_tag="ACHK49_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO9264831.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2619..3287)
                     /gene="hxpB"
                     /locus_tag="ACHK49_004305"
     CDS             complement(2619..3287)
                     /gene="hxpB"
                     /locus_tag="ACHK49_004305"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO9264832.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3434..3970
                     /gene="yniB"
                     /locus_tag="ACHK49_004306"
     CDS             3434..3970
                     /gene="yniB"
                     /locus_tag="ACHK49_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO9264833.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4011..4871)
                     /gene="yniA"
                     /locus_tag="ACHK49_004307"
     CDS             complement(4011..4871)
                     /gene="yniA"
                     /locus_tag="ACHK49_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO9264834.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4977..5267)
                     /gene="ghoS"
                     /locus_tag="ACHK49_004308"
     CDS             complement(4977..5267)
                     /gene="ghoS"
                     /locus_tag="ACHK49_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO9264835.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5368..6297)
                     /gene="pfkB"
                     /locus_tag="ACHK49_004309"
     CDS             complement(5368..6297)
                     /gene="pfkB"
                     /locus_tag="ACHK49_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO9264836.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1624 a         1580 c         1550 g         1556 t
ORIGIN      
        1 tgcgattcaa aaaaaatgag agtctcctaa agtttccttc gataatatca atgcttaata
       61 tcaatcttct ttttaatcca ctaaatcttc cgggaatggg aagtggtgtg cttgaagata
      121 ttatgagtat tccagatagt tcacttagaa aacgtctcgg gtatgaggtt ttaagcttct
      181 cactacaggc acattcattg agccaggaat gtattgataa gttggacatt tttttcgctg
      241 acgatctgtt taaatatgag tcagtatgca tcgccgcgat ggaacattta aaaagtaaag
      301 caacggcccc tatacaaaac ggaccgttgc cagcataaga attaacggtg tgccagttcg
      361 gcgtcgtctt cgctatccag aatggcttta tcggtttgct tcagccactg gctggtcagc
      421 gtgccagctg tcatcgagcc actaacgttt agcgccgtac ggcccatgtc gataagcggt
      481 tcaacggaga ttaacagcgc caccagggtt actggcaggc ccatcgcagg cagcacaatc
      541 agtgcggcga aagttgcacc accaccgaca ccggcaacgc ctgcggaact aacggtaaca
      601 ataccgacca gcgtcgcaat ccacatcggg tccagcgggt taatgccaac cgtaggcgca
      661 accatcaccg ccagcattgc cggatacaaa ccggcgcagc cgttctgacc aatggttgca
      721 ccgaaagagg cggcgaaact ggcgatggat tcaggaacgc ccagacgacg cgtttgtgct
      781 tccacattca gtgggataga cgcagcactg gaacggctgg taaaggcaaa cgtcagcaca
      841 ggccatacct tacggaagta cttcagcgga ctcacgccat taatgcccag cagaatgcca
      901 tgcactgcaa acataatcag cagaccgagg taggacgcga caacgaaact tcccagtttg
      961 atgatgtctt gcaggttaga acctgcaacc actttggtca ttagtgccag aacgccgtaa
     1021 ggggtcaact gcatgaccag gcgaaccagt ttcatcaccc agctttgcag ggtatcgata
     1081 gcggttaaga cgcgttcacc tttcggcgca tcatccttca gcagtttcag cgcagctaca
     1141 ccgaggaatg cggcaaaaat taccacgctg ataattgacg tcggattggc tccggtcaga
     1201 tcggcaaacg ggtttttcgg gataaaggac aagaccagct gcggaacgct caggtcagag
     1261 actttaccaa catagttact ttcgatggcg ttcagacgtg cagtttctgc acctccctga
     1321 accagacctt cagccgtcaa accaaacagg ttggtgacca gcacaccgac cagcgccgca
     1381 atcagtgtgg taaacaaaag cgtaccgatg gtcagaaaac tgattttgcc taactgagat
     1441 gcgttatgca gacgggcaac cgcgctcaga atagaggcga agactaacgg cataacgatc
     1501 atttgcagca gttgaacata gccgttacca acgatgttaa accactgtac agaatcttta
     1561 agtacctggc tgtcagaacc ataaatggta tgcagggcaa ggccaaaaac cacacccatc
     1621 accagaccca ccagcacttt tttcgccaga ctccactgtt tgtggcgggt ctgagccagc
     1681 gcaaacagca gtacaacgaa caccacgatg ttcgcaatta atggaaagtt catccccgtt
     1741 ctcctgatct tattatttct atcactgcgt agcagtcata gtgttggcgc aagattaaca
     1801 gaactgtgag ctggctctta tattcaaatg gaattgctta ttccagaaag gtgatttttg
     1861 cctttttagt attcaacctg atgcttaata agccgatgaa actggatctg caaggtattg
     1921 atcatttgcg atgaccaacg aaccgcgctg ttttcgggta atgaatgggg catccatacc
     1981 gaccagacaa ataatgccat gcagataaaa cgttccagtt ggttgccctt ttggggaacc
     2041 aggataggca agcggaaacg ccagcgacat ggccagagca ggggaacacc ggcgggtgtc
     2101 agcatatcgg caagtatgtg gcttaaataa cccagcacca ttccttgtag cgcatcagcc
     2161 ggaatgaacc agctttcagg aaccttaagg taaaaggttg ccagcagcgc aaataccgcc
     2221 agcagactgt gggtaaaacc acgatgccca aaagcgcggg cgatcggttt tgatatccac
     2281 tttaatcgct gcccaagaaa cgactttggg tgatcgatgt ccggtaacaa acacgtcagg
     2341 attgcggaag ggacaatatg ccaccagtca ccctgtgcca gcacgggcgt cagctcggca
     2401 tttttggcaa ataccgcaca agcaatagaa aagagaaggt gaccttccgc cgtcatgata
     2461 aaactcacta aactgtcgat acgtacagta taggattttt atacagtgca cggaagtggt
     2521 gacggtgtaa ctctttctct taaattgacg attaatccgt cagttgaatg caaattcaga
     2581 taaagaacca ggggcagcga gatcactgcc cctggtcatt aaccgagaag gtcttttgcg
     2641 gtgagttctg tcagcgatga gagtttgacg tttgctaata caaaacgtgg atcattttgc
     2701 gcttctggcg caggaacgac aatggaacgc atgcgggctg ctttagaggc gatcatgcca
     2761 tttactgaat cttccagcgc tacgcaggtc agtgggtcaa cgcccagttt tgctgcgcag
     2821 tcgagatata cttgcggatg cggcttgctg taaggcagtt tttcagccga ggcgagggca
     2881 tcgaaactgt cgcgtaagtc aaacatggtc aacacttttt ccagcatatg tagtggtgac
     2941 gcggaggcca gtcccaccaa taaaccttgt tctttgcata acgcaacggc ttcgcgcacg
     3001 cctggtaata atggacgtgt ctcttcaacc agtgaaatgg cacgggcaat aacccgttct
     3061 actacttcct gacggcttgg cccattccac ggttgccggg cgtaccaaag atcgaccacc
     3121 atatcgatgc gtaaacctaa ggtgtccggc agctcgttac gacgggagat atccaccccc
     3181 aggcttgcca tcacatccag ttcggctcga tcccataaag gttctgagtc gataagtaat
     3241 ccatccatat caaaaattgc agcaagaatc tgacgcgggg ttgacattac aacctctcct
     3301 ttagagcatt atgtgtaagg tattaagccg tgcttatgat taagcgccta tcatatcgcc
     3361 tttaaagaat gggcgaaaac agatatctac aggtaaacta agtgaaacga cgcgtcttaa
     3421 tcaaggagaa ctcatgacgt atcaacaagc tggacgcatt gctgttttga aaaggatttt
     3481 gggttgggtg atttttatac ctgcattgat ctctacattg atttctttgc tgaaatttat
     3541 gaatacccgg caggaaaacc aggaaggcat taatgcggtc atgctcgact ttactcatgt
     3601 catgatcgat atgatgcagg cgaatacgcc ttttttgaat ctgttctggt ataactcccc
     3661 gacgcccaat tttaatggtg gcgtgaacgt tatgttctgg gtgattttca tcctgatttt
     3721 tgtcggactg gcgttgcagg attccggtgc ccggatgagt cgccaggcgc gcttcttgcg
     3781 ggaaggtgtt gaagatcaac tcattctgga aaaagccaaa ggggaagagg ggctaacgcg
     3841 tgaacaaatt gagtcccgta ttgttgttcc gcaccatact attttcctgc agtttttctc
     3901 actatatatc ctgccggtta tttgtattgc tgcaggttat gtgttctttt ctctgcttgg
     3961 gtttatttaa tcaaaaggcc gcacggagcg gccattatcc tcaacccata tcatgctgct
     4021 aataatctat ccaatgactg ctgagcaata accaaatgct gaccgccaaa taatcttgca
     4081 cgatttaaca gcgtgtagag ttggtaaacc ggttgacgtt caaggaaatc ggcaggtagc
     4141 ggggatactg actgatagcc gtcatagatt tgtggcggct gttcagtatg cagcggtaac
     4201 atcgccaggt cgcactctcg gtcaccccag tagcaggcag ggtcgaaaat gtacgggcca
     4261 tccggaccca gcgcacagtt gccggaccat aaatcgccgt gcaacagaga aggctgcggt
     4321 tgatgcgagg ccagacgctg ctgaatatgc tcgacgatgg cgtcgatatt gccgaaagcg
     4381 atccctttct ctgctgccag ttccaactgc cagccaatcc gttgttcagc aaaaaacgtt
     4441 gaccagcgac gttgccaggt gttgggctgt ggagttgtgg agagcgagtt atcaaaatcg
     4501 aggccaaatt gtggttggtc actccattga tgtaaacgcg caatttgctg accaagaata
     4561 aatgcgctat gcgcatccag cggacgaggt gggagataat ccatcaccag aaaactgtag
     4621 tcacggtcag cgcctactgc ccaaacctta ggcacggtga cggttttact acgcgacagt
     4681 aactccagtt ggtcggcttc ggcggtaaaa ccgggaagca gttccctttc atcacatttg
     4741 acgaaaaagt catgtcctgc atagcgcaaa tgccatgcgg catggacttc tccgccaggc
     4801 agttcattac gcagttcgat ttcgccttca cctaactgct cgctcaaaag acgactgatt
     4861 gcctgccaca tgatgtctct cccatgttgt ctgccagatc ataaggttag cgcaagaatg
     4921 cggtgtaaaa acacgagcta acgcacacca cagactgatt attgcccaaa aaccatttat
     4981 tttctgttgc tattccattc ctcccaggtc gtgatggtga tttcaggatc tttgccggtt
     5041 gcactttggg taagccccga aaccagttct ctaatttctt cttcactttg ggtactgata
     5101 agtccaaaag tgttagtccc cagctcatgg atatttccct catcatccgt catggtgagc
     5161 aaaaaaccgt cgcgagtcag gtaattattc aactcgttaa tttcagtcaa tgtatcctca
     5221 tgcaacatga cggttatgac ataacggaca agatcgccac tggccatagt tcaccccttt
     5281 gttatcacat gacttttgag catagtcgga gaaacgcgtt gccgacaggt tggtggtgat
     5341 tcccccaatg ctgggggaat gtttttgtta gcgggaaagg taagcgtaaa ttttttgcgt
     5401 atcgtcatgg gagcacagac gtgttccctg attgagtgtg gctgcactcc ccgcagctac
     5461 gccaaaacgg accatctctt caagagaggc attttctgcc agtttcagtg tcatcgcgcc
     5521 gaccatgctg tcaccagcgc caacggtact ctggcttttc accggtggtg gcaccacctg
     5581 aatgcagttt tcactatcaa cacccagcgc tccttgtgga ccaagggaaa cgacaacccg
     5641 tttggccttg ccgctattaa cgatttcctg cgcggctttg cggacatcgt ctggctgggt
     5701 gagttcgcga ttcaccagcg cactgagttc tttttggtta ggcttaacca actcgatgtt
     5761 accaatcgcc agtgctgcac ttaacgcttc gccagaactg tcgatgatgc agcggatccc
     5821 ttggttttgc gcagcggaaa tcagttgggt taatttttcc agcttcacac ctggcggcag
     5881 gcttccgctt atgaccagga tggccccgga ttcaatttcc agaacttgct cttcaagctg
     5941 gcgaaactca tcttcattta atgccgcgcc tggcataaca aaacgatact gctcaccgct
     6001 tgcttccaca tgtacgtgta aattctgccg ggtccagtct ttggcttcta cagtagcgac
     6061 ggggacattt tcatccgcca acagagaaac caggtgttcg ccggtcgcgc cacccgccgg
     6121 gaagatcgct gtggcactgc ctccaagatg ggcaatggcg cgggcgacgt tgatgccgcc
     6181 gcccccgggt tcgaacaccg gtgcggtaca gcgcagtttt ccttcgggat aaatttgcgg
     6241 ggtaattgtt gcgctatcga gagagggcgc aagtgtcaac gtatagatac gtaccatcat
     6301 ttcctcctat
//
LOCUS       DAZGLJ010000249         6309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000249 DAZGLJ010000000
VERSION     DAZGLJ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.249"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(57..443)
                     /gene="ridA"
                     /locus_tag="ACHK49_004310"
     CDS             complement(57..443)
                     /gene="ridA"
                     /locus_tag="ACHK49_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO9264837.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(516..977)
                     /gene="pyrI"
                     /locus_tag="ACHK49_004311"
     CDS             complement(516..977)
                     /gene="pyrI"
                     /locus_tag="ACHK49_004311"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO9264838.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(990..1925)
                     /gene="pyrB"
                     /locus_tag="ACHK49_004312"
     CDS             complement(990..1925)
                     /gene="pyrB"
                     /locus_tag="ACHK49_004312"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO9264839.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(1929..2063)
                     /gene="pyrL"
                     /locus_tag="ACHK49_004313"
     CDS             complement(1929..2063)
                     /gene="pyrL"
                     /locus_tag="ACHK49_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO9264840.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(2344..2739)
                     /locus_tag="ACHK49_004314"
     CDS             complement(2344..2739)
                     /locus_tag="ACHK49_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO9264841.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(2870..3583)
                     /gene="bdcA"
                     /locus_tag="ACHK49_004315"
     CDS             complement(2870..3583)
                     /gene="bdcA"
                     /locus_tag="ACHK49_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9264842.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3654..4247
                     /locus_tag="ACHK49_004316"
     CDS             3654..4247
                     /locus_tag="ACHK49_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO9264843.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKVGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            4392..4844
                     /gene="tabA"
                     /locus_tag="ACHK49_004317"
     CDS             4392..4844
                     /gene="tabA"
                     /locus_tag="ACHK49_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO9264844.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            4967..>6309
                     /locus_tag="ACHK49_004318"
     CDS             4967..>6309
                     /locus_tag="ACHK49_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264845.1"
                     /translation="MAKISDLNCYQHMASADNVKPKNEVLNTWCVGMNDFARNAEGQD
                     SRRNILSPGAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMNF
                     VMNNPEGERADSSKVIIEISLSTTTTMGTRQGHTAIIFPQSDFLSFRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKNMALLLNNHQLNWKDAIDYNELLEKPLASTLEKIKKEH
                     LLLMPHVCDDTIPYLLGEGGILEEINDLKALHDTRIASGTEGNDEINNIKINLSNILI
                     DSLDDAKVNLEKVIDSMLKTFLKLPYINDVKILEWCFNQSMQYSDDTTKIKHARSVID
                     HIDFNCDQSKIAETLFFNLNKEPYKNSPELQELIWEKLVVYVNDFNLSNQEKSRLILR
                     LFDDVKLLFNEVPVSILVNDIFLKDFFMKQPDFAKWYFYQLIKKYEGEQLYLNELG"
BASE COUNT         1687 a         1395 c         1513 g         1714 t
ORIGIN      
        1 ttttgcgttt gtcatcagat ttaatccggg catgatagcc cggatttcca tcaagattag
       61 cgacgaacag cgatcgcttc gatctcaatt tttacgtctt tcggcagacg ggcaacttca
      121 acgcaagaac gtgccgggaa ggtggcattg tgttcggtga agaaggcttc gtaggtagca
      181 ttaacggttg cgaagtcgtt cagatctttc acaaacacgg tggttttaac gatgtcgccc
      241 actttcaggc ctgcggcttc gacgatcgct tttacgttat ccagcgactg acgtgcctgt
      301 gcagcgacgt ctgccggtac ttcgcccgtt ttcggattta ccgggatctg gccggaggtg
      361 atgatcatat tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt
      421 tccgtcgcga tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc
      481 attataggga gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg
      541 gaaaactctt tttcacagta tttgcatttg agcgcgatgt cattggcgcg ttttcgcacg
      601 gcaaagctgg aagaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc
      661 acattgtcga tgcgctccgg cagactgggg cgcgatttac ccaccacttc atagttgtcg
      721 atacggttaa ccgtggcttg cggcgcatac aatgccagtt gatctacctg atcttcactc
      781 aaaaaggtat tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaggttc
      841 agaccaatgg tgatgcgctg atccgtttcg gtcagcttga acagactcaa cagcttaaaa
      901 ccgatctggg cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat
      961 ttattatcgt gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt
     1021 gccagtaacg cctggcgagc gaaaatcccg ttgcctgcct gctggaagta ccaggcgtgt
     1081 ggcgttttat caacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc
     1141 atattggctt tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg
     1201 gcgtactcgg acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact
     1261 tccgccatca cttcttcaat agagctgtgc agactccatg cgatcccttt ttcatcgagc
     1321 atatccagaa tgtattgcgg cattgccagc gcgtccggcg cgatgaagta aaaacggttg
     1381 ccatcgaact tcgctaacgc ctgagtcagg gagtgaacgg tgcggccata tttcaggtca
     1441 ccaaccattg cgacgtggag attgtccaga cgcccctggg tttcctgaat agtgaataag
     1501 tccagcaagg tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca
     1561 ttgccggaaa actcggtggc caggcgcgcc gcaccttcct gcggatgacg catcactatc
     1621 gcatcgacgt aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ctttttaccc
     1681 agtgatgtat tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatagat
     1741 gtttcgaaag agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg
     1801 tgcttcaaca gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga
     1861 ttaaggtcat cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta
     1921 gccatctttt atctcctgac gcctgggcaa aaaaaagccc ctcgattgag gggctgggaa
     1981 tgggtgatca acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca
     2041 aaatgtcgaa cacactgaac catacatcct cccggcaaat tgtccggcat tatactcata
     2101 gccagaagcg gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat
     2161 taaaagtcaa aatgtatgca tttttatttg ctttgattct ggaggagaac tgtcgttcgg
     2221 tatcaataat tgcagacatt tcctgctttt ttctttttca ctttcacgca atcagatatg
     2281 cattttattc cattcatccg acttataggg cggagtgtga aaacgaacgg ctaacactat
     2341 tgcttacagc tcagggatgc gcgctatcac tttaatttca aaatcaaagc ctgccagcca
     2401 ggtaacgccc accgccgtcc agtttggata aggaggggcg ctaaatattt catttttgac
     2461 cgtcatgatg tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat
     2521 atcgtcaaaa gtgcatcccg cagcagccag ggtcgcatgc aaattatcaa atgccagtct
     2581 gacttgttgc tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga
     2641 aacaaacagc aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga
     2701 atgtcggcca gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac
     2761 gacatgaaca gaagatttct ctttaccggg agccgctttt agcggacgac gtgagtaaac
     2821 aaaacccaga catcatggat aatggctggg cttaattgag cgtagtcggt tatgcgccaa
     2881 acgcgccatc aatggtatgc atcgcgccgg taacaaaact ggcttctggc cctgctaacc
     2941 atgcgaccat accagcgacc tcttccggtt gcccatgtct tttgatagcc atcaaactat
     3001 gcaacatatc gcgcattggc ccgttggcgg gattagcgtc ggtatcaatt ggccctggct
     3061 ggacgacgtt aatggtgatc ccacgcggtc caaaatcacg ggccagcccg cgcgccatgc
     3121 cttgcagggc agatttgctg gcggcataag cagccatgcc tgcaacaggc atacgatcgc
     3181 cattcacgga gccgatgatt aagatgcgcc cgccttcggg catctgccgg gcggcttcaa
     3241 cagaggcatg ataaggagca tgaatattga ttttgaaaag gcgatcaata tcgtcggcat
     3301 ttaattccag ggcatcgcca aagacgccaa tacctgcatt taccaccagg atatccaatg
     3361 cgccgctctt acgaacgaca tcaatgacag cgtctctgtc agcactatct gtaaatactg
     3421 ctgtcgctcc agtctcttgt gccagatgtt cagcggcatc tttcgacccc gcataggtga
     3481 atcgtacatt ggccccatca gtgacgaaac gacgtacgat agcggcaccg attccacgac
     3541 tgccaccgag gatgagaact gtcttacctg taaaagcgcc cataaggact ccttgattta
     3601 ttatgtaaca tgcattacaa aactgtttta actttctgtc aacaagtttt gtaatggtca
     3661 ctaaaaaaca atctcgcgtt ccaggtcgtc ccagacgttt cgctcctgag caggcagtct
     3721 ctgcggcaaa agtgcttttt caccaaaaag gtttcgatgc tgtcagtgtt gctgaagtta
     3781 ctgattatct tggtattaac cccccgagcc tctacgcagc ttttggcagt aaagttgggt
     3841 tatttagccg tgtactcaat gaatatgtcg gtacggaagc tattccgctt gccgatattc
     3901 ttcgtgatga tcgtccggta ggcgagtgcc tggttgaggt attaaaagaa gcggcgcgca
     3961 gatatagcca aaacggcggc tgcgctggct gtatggttct tgaaggtatt catagtcatg
     4021 atccacaagc gcgtgatatt gccgttcaat attatcacgc cgcagaaacg accatttatg
     4081 actatatcgc caggcggcat ccacaaagcg cacaatgtgt gactgatttt atgagtaccg
     4141 tgatgtcagg gctttctgcg aaggcacggg aggggcactc catcgaacaa ctctgtgcaa
     4201 cagctgcact ggcgggggaa gcgataaaaa ctattctcaa ggagtgatgc tggccttgac
     4261 ccgaaaggcg ggaacgcgcc tgccgataag ttgtgataag acaataattc acgcgttaag
     4321 gctagcggaa ttgatcatct tttcgtataa cggtggaaat gaaacattgt tttaattaag
     4381 gagtggaaaa gatgatcatc ggaaatattc ataatcttca gccgtggcta ccccaggagt
     4441 tacgccaggc gattgagcat atcaaagcgc acgttacggc agaaacgcca aagggtaagc
     4501 acgatatcga aggcaatcgt ctgttttatc ttatctcgga agatatgacc gagccgtacg
     4561 aagctcgccg tgcggagtat catgcccgct atctcgacat tcagattgtg ttaaaaggtc
     4621 aggaaggcat gaccttcagc acgcaacctg caggcacgcc ggataccgac tggttagctg
     4681 ataaagacat cgcatttttg ccggaaggcg ttgatgagaa aacagttatc cttaatgaag
     4741 gtgattttgt tgtgttttat ccgggggaag tgcataaacc gctgtgcgca gtgggcgcgc
     4801 cagcccaggt tcgcaaagca gtagtgaaga tgctgatggc gtgatgactt ttcgccgtaa
     4861 ataactccag gtttacggcg agtttgtgaa aagagcgttt tttgatattt ttttgtgagt
     4921 agaatttgta atgcttagac gttcttatta actcaaggag tccgtcatgg caaaaatatc
     4981 agatttgaat tgttatcaac acatggcatc agccgacaat gttaaaccaa aaaatgaagt
     5041 cttaaatacc tggtgtgtgg gaatgaatga ttttgctcgt aatgccgagg ggcaggatag
     5101 cagaaggaat attctatctc ctggagcatt tttggagttt ttggcaaaaa tatttaccct
     5161 gggttatgtg gattttagca aacgctccaa cgaagcgggt agaaatatga tggctcatat
     5221 tgagtcctca tcttatatca aaaataatga tggcagtgaa ataatgaatt ttgttatgaa
     5281 taatcctgaa ggtgagcgtg cggattcatc taaggtgatt atagaaattt cactttctac
     5341 taccactact atggggactc gtcaaggaca tacagccatt atatttccac aatctgattt
     5401 tttgagtttt cgttatgaag gtaagtcttt tgaaagaaaa gatgatagtt cattacatct
     5461 gattactaac aaggttctgg cgtgttacca acgcgaagct aacaagaata tggcgcttct
     5521 attaaataat catcagttga actggaagga cgcaattgat tataacgagt tattagaaaa
     5581 gccattagcc agtacattag aaaaaataaa gaaagaacat ctgctattaa tgcctcatgt
     5641 atgtgatgac acgattccat acttactagg cgaaggtggt atacttgaag aaataaatga
     5701 tctgaaagcg ttacatgata ccagaattgc tagtggtaca gaaggtaatg atgaaattaa
     5761 taacatcaaa attaacctgt ctaatattct tattgattcc ttagatgatg caaaagttaa
     5821 tctcgaaaag gtcatcgatt cgatgttgaa gactttttta aaattgccat atattaatga
     5881 tgtaaaaatt ctggagtggt gttttaatca aagtatgcaa tattctgatg atactacaaa
     5941 gataaagcat gcccgctccg tgatcgatca tattgatttt aactgcgatc agtctaaaat
     6001 agctgagaca ttatttttca acctcaataa agaaccctat aaaaacagcc ctgagttaca
     6061 ggaattgatt tgggagaagt tggttgtata tgtcaatgat tttaacttaa gcaatcaaga
     6121 aaaatcacgc ttaatactaa ggttatttga tgatgttaag ttgctattta atgaagtgcc
     6181 tgtcagcatt ttagtaaatg atattttttt gaaggatttt ttcatgaagc aacctgattt
     6241 tgcgaagtgg tacttctatc aattaattaa gaagtatgag ggcgaacagc tgtatcttaa
     6301 tgagttagg
//
LOCUS       DAZGLJ010000250         6298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000250 DAZGLJ010000000
VERSION     DAZGLJ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.250"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..738)
                     /locus_tag="ACHK49_004319"
                     /pseudo
     CDS             complement(1..738)
                     /locus_tag="ACHK49_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
     gene            complement(805..1089)
                     /locus_tag="ACHK49_004320"
                     /pseudo
     CDS             complement(805..1089)
                     /locus_tag="ACHK49_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459368.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1613..2587
                     /gene="hemB"
                     /locus_tag="ACHK49_004321"
     CDS             1613..2587
                     /gene="hemB"
                     /locus_tag="ACHK49_004321"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO9264846.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(2693..3544)
                     /gene="tauD"
                     /locus_tag="ACHK49_004322"
     CDS             complement(2693..3544)
                     /gene="tauD"
                     /locus_tag="ACHK49_004322"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO9264847.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTIAPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3541..4368)
                     /gene="tauC"
                     /locus_tag="ACHK49_004323"
     CDS             complement(3541..4368)
                     /gene="tauC"
                     /locus_tag="ACHK49_004323"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO9264848.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(4365..5132)
                     /gene="tauB"
                     /locus_tag="ACHK49_004324"
     CDS             complement(4365..5132)
                     /gene="tauB"
                     /locus_tag="ACHK49_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO9264849.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(5145..6107)
                     /gene="tauA"
                     /locus_tag="ACHK49_004325"
     CDS             complement(5145..6107)
                     /gene="tauA"
                     /locus_tag="ACHK49_004325"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9264850.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1497 a         1734 c         1576 g         1491 t
ORIGIN      
        1 gatacagcca gccttcatct gtacgtaagt acgtgatgtc tcctgcccac ttctggttcg
       61 ggccactggc gtaaaaatcc tgctccaaca gattttctga cacaggcagg ccgtgtgcgc
      121 ggtagctgac cgggctgaac ttccgggagg cctttgccct cagtccctga cggcgcaggc
      181 ttgccgccac ggtttttacg ttaaaggggt aaccctgagc acgcagttca tccgtcaggc
      241 gtggggcacc gtaacgctgt tttgaccggg taaaagccgc gaggacaacg ctgtcgcagt
      301 gttggcggaa ctgctgacgc gtgcttatcc ttgtccgccg ctgacaccac gtataccagc
      361 cgctgcgggc cacccggagc acgcggcaca ttgctttgat gctgaactca gcctgatgtt
      421 tttcaataaa gacatacttc atttcaggcg cttcgcgaag tatgtcgcgg ccttttggag
      481 gatagccagc tcttcatccc gttctgccag ctggcgtttg agacgtgcaa tctcggtaga
      541 catctccagt tcacgttcag aagacgtctg ctgattttgc tgtttactgc gccagttgta
      601 gagctgtgat tcatacaggc tgagttcacg ggctgcggca gtaacaccga tgcgttcagc
      661 aagcttcagg gcttcactgc gaaattcagg cgaatgctgt ttacggggtt ttttactggt
      721 tgatactgtt tttgtcatgt gagtcacctc tgactgagag tttactcact tagccgcgtg
      781 tccactattg ctgggtaaga tcaagtcatg attcaggtaa tatgtagaga tagtcccgtt
      841 aacagtgtca ccattaaagg tggtatcgaa atcaccgttt gtgttgttat agtagacaaa
      901 gccactataa ttcacatagt taccatctac agtacccggt gcatccatat tgctccagtc
      961 aagagcggta gttggattgt cgtaataagt ccaggtgtca taagtagttg cattagcctg
     1021 agaggtgata gccagagtgc aagccaatgc taattgtgat actacaagtt tctttttcca
     1081 ggagtgcatt tgtcatccct cctcagggac gtaataagtt gatccatcaa ttatcaatgc
     1141 atagaaaaca tattatgttt cgcgctaatg attatgctgc gttacagacg aatggttcaa
     1201 ttattctttc ccctaagtcc gaatgtggac atatataacc tatggagaaa aacacgcatc
     1261 aaattgataa aagggcgata tttcaacatg ttgcagataa tgttattttt gatacatacg
     1321 cgttataaat atcaaaaaca caaaccagat aaatatctta tcaatataca acaaagtttg
     1381 aattattcaa tttatagcag cacaaccaaa cgccaaaaaa taataaccaa gagctataaa
     1441 taaagattaa aaatattttc agtgaatatg aaattattgc tatttctatt acaaagatgt
     1501 cctggtctct ctttcattct cttcttgtca acatcacgac aactttcgta aaacactcct
     1561 gccccgcttc aggtatactg tgcaccttaa ttccacaaac atcaggcaga ccatgacaga
     1621 cttaatccaa cgccctcgtc gcctgcgcaa atctcctgcg ctgcgcgcta tgtttgaaga
     1681 gacaacactt agccttaacg acctggtgtt gccgatcttt gttgaagaag aaattgacga
     1741 ctacaaagcc gttgaagcca tgccaggcgt gatgcgcatt ccagagaaac atctggcacg
     1801 cgaaattgaa cgcatcgcca acgccggtat ccgttccgta atgacttttg gcatctctca
     1861 ccataccgat gaaaccggca gcgacgcctg gcgggaagat ggactggtgg cgcgtatgtc
     1921 gcgcatctgc aagcagactg tgccagaaat gatcgtcatg tcagacactt gtttctgtga
     1981 atacacttct cacggtcact gcggtgtgct gtgcgagcat ggcgtcgaca acgacgcaac
     2041 tctggaaaat ttaggcaagc aagccgtggt tgcagctgct gcaggcgcag acttcatcgc
     2101 cccttccgcc gcgatggatg gccaggtaca ggctattcgc caggcgctgg acgctgcagg
     2161 ctttaaagat acggcgatta tgtcgtattc gaccaagttc gcctcttcct tttatggtcc
     2221 gttccgtgaa gctgccggaa gcgcattaaa aggcgaccgc aaaagctatc agatgaaccc
     2281 aatgaaccgt cgcgaggcga ttcgtgaatc actgctggat gaagcccagg gcgcagactg
     2341 cctgatggtt aaacctgctg gagcatacct cgacatcgtg cgtgagctgc gtgaacgtac
     2401 tgaattgccg attggcgcgt atcaggtgag cggtgagtac gcgatgatta agttcgccgc
     2461 gctggcgggt gctatagatg aagagaaagt cgtgctcgaa agcttaggtt cgattaagcg
     2521 tgcgggtgcg gatctgattt tcagctactt tgcgctggat ttggctgaga agaagattct
     2581 gcgttaattt ttttcctctc agaaaggggg agttaacctg ttgaaagtac ggtgaacgcc
     2641 ccctcaccct aaccctctcc ccaagggggc gagggaaccg tccgcactcg tatcaccccg
     2701 cccgataaaa cggtttatcc ccaaggatcg tcgcccgatg cataatccgc cgctgtggca
     2761 ggtaatcggc attggcatag tgctgagtca cgcggttatc ccaaatcgca atatcattcg
     2821 gttgccagcg ccaacgcacc tgaaactccg gtttagtgat atgggcgaat aaaaagccta
     2881 acaaggcttc gctctctttc tcgctgacat cgacaattcg cgtagtaaag ccttcattca
     2941 caaacagcgc ctgtttaccg ctcaccggat gcgttcgcac caccggatgt agcaacggag
     3001 gatttttcgc gaccgcctcg cgccagcgtt gatgttcctc ctcggttttg cggtatttgt
     3061 attccgggaa cgatttacgg aaatcatgct ccgcacgcag cccgctcagc agctggcgga
     3121 agggaacaga gagcgcctca taggccgcaa taccgctggt ccagagcgta tcaccgccgg
     3181 tcgaaggtaa ctctttagct gccagaatcg cccctgcggg tggcgtttca ataaatgtca
     3241 catcggtatg ccagttgtcg ttatccggcg gattatcgtt atgggtatcc agcacgatga
     3301 tctcgtcaac cccttcggca tgagggtaaa cagggtgaat atgcagttcg ccaaaacgct
     3361 gggccagcgc gcgttgctgc tgcggcgcaa tagtttgatc gcgcagaaac accacctgat
     3421 ggcgcagcac cgcatggtaa agctgttcaa actgattatc gcttaacggg cgcgtcaggt
     3481 cggcacccga aatttgtgcg ccgatatacg gccccagcgg ggtaatgctc agacgttcac
     3541 tcattgtact tctccatgcc agggcgtcag acggcgctgt aacgcgcgca aacccagttc
     3601 taaaagaaag gcgataatcg caatcaccgc gatccccgcc agcaccacgt cagtcgcgag
     3661 aaattcaccc gctgactgaa ccataaatcc taaaccgcgc gtcgcggcaa tcagctccgc
     3721 cgctaccagc gtagaccagc ccacacccag accaatgcgt aatccggtga ggatttccgg
     3781 cagcgcgccg ggcaaaatga caaaccacag cacctgcgca cggctggcac ccagcgactg
     3841 ggcggcacga atgcgaacct gctgcgcgct tttcaccccc gccagcgccg acatcgccac
     3901 cggtgcaaaa atcgctaaat agatcagtaa gatcttcgag gtttcaccaa taccaaacca
     3961 gatcaccatc agcggcaaat aagccagcgg cggcaccgga cgataaagtt cgattaccgg
     4021 atccagaatg ccgcgcaccg tcgggcttag ccccatggcg atcccgaccg gaataccgat
     4081 caccaccgcc gcgagtagcg ccagcacaat gcgcgtcagg ctggctgcca gatgctgcca
     4141 cagcgtggcg tccataaatc cctgcggtcc ggcaatggtg agcagttttg ccagtacttg
     4201 ttgcggcggc ggcaaaaaca gcgggctaat cagttgcagc gccgccaccg tccaccatac
     4261 agtgagtaaa accgctaacg tgccaatgct taaggtcacc tgacgcgaga gcggccagcg
     4321 ccatttcagc cgccgcgaat gcagtttttc attaatgagc acactcatga gaacgcctcc
     4381 cgttgctcaa atacgcggct taaaacatat tcgcgcatgg cgatgaattg tggatcggac
     4441 ttgatgctgc ggctcgactc tcccgcaaca aagcggcgag caaagttgag cggcagccgc
     4501 tccagcacgc ggccagggcc gggtgaaagc agaaccagtt cagtcgccat aaacaccgct
     4561 tcttctatat cgtgggtaat caacagcacc tgcttgcccg tctcctgcca gagtttcagc
     4621 agcagggttt gcatctggtc gcgggtgaag gcgtccagcg caccaaacgg ctcgtcgagt
     4681 aataacagct ggggattcgc cgccagcgcg cgggcaatcc ccacccgctg acgttgacca
     4741 ccggaaagct gccagatata gcgtttttct gcgccttcca gccccacttt tttcagcatc
     4801 tggtgcgcga tttccagtcg ctgcattttc tctatacctg ccaattgcag gccgaacgcc
     4861 acgttgtcct gtacattgcg ccacggtagc agcccttcat tctgaaaaac caccccgcgc
     4921 tctgctcccg gtccctcaat acgcttaccc gccagttgaa tgctgccatg ctgataaggc
     4981 acaaaaccgg caatcagatt cagcagggtg gttttaccgc agccggacgg ccccagcacc
     5041 accagtagct cgccgctttc cagcgtcagg ttgatatctt ccagtgccgg tttgccgcca
     5101 taatcggcgt aaagatgaga gatttgcagc atctgcgcct ccttttattg cacgaagcgc
     5161 gaggtaacgt actggctgta atcattcgct acagccggga ccttgccctg ctctttcaaa
     5221 aactgcgcgg tgtcgatgat cgctttgttc accggtccgg tcagttctgc cgtttgttgc
     5281 tgcggcgtca gataggtatt ccccttcacc agccccggaa cgtcaccttc cggcacgccg
     5341 cttaaacgcg ccagtttgct gatgttttcc ggctgtttca gccacgcgtc tgggttagca
     5401 atgtacggtt gctgagcatc gatggcgctt ttagcgaacg ctttcacgac ctcaggatgt
     5461 ttctcggcaa aatctttgcg caccacccag acgtccagcg ttggcgcacc ccactgcccg
     5521 acctgttcag aatcggtcag caccttgccg tctttttcca gggcgttaac cgccggtgcc
     5581 cagacataag caccatcaat atctccccgc tgccaggcag cgataatcgc gggcggctgc
     5641 aggttcacaa tctccacttg cccgggttta atgccccagt gtttcagcgc cgccagcagg
     5701 ctgtagtggg tggtagagat aaacggtacg gcgatgcgtt tgccaatcag atcttccggt
     5761 ttgctgatag ttttctttac caccagcgct tcggagttac ccagttttga cgccagcaag
     5821 aaaacttcaa tcggcacctg ttggctggct gcaaccgcta acgggctgga accgaggttg
     5881 ccgatttgca catcgccaga agccagcgcc cgcacgatgc tggctccgct gtcaaactta
     5941 cgccagtcca cggttgctcc gctttcttta gcaaaggtgt tgtcggcctg agccactttc
     6001 gccggttcgg ctgaggtttg atacgcaacg gtgacgttca ccgcctgtgc ctgaaaagcg
     6061 atgaatgcca gtgcggcaag aagtgtgtta cgcgatgaaa ttgccatgat tgtctgctcc
     6121 cctgtcttgt tatgtgagca gtattcagga ataaaaacat tcattaaaag aattagtcgt
     6181 tatcgcacag atgattttat tcttagcaaa aaaacggcaa aggaaagatt aaatatgcaa
     6241 ttactccgtt ctaacaattg ggaaaattac tacacttcat tttagtgatg cataaaca
//
LOCUS       DAZGLJ010000251         6261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000251 DAZGLJ010000000
VERSION     DAZGLJ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.251"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..796
                     /locus_tag="ACHK49_004326"
     CDS             <1..796
                     /locus_tag="ACHK49_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO9264851.1"
                     /translation="PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSIN
                     ENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYAGIKDYPVCFYGPKYKIMANHHGIT
                     FDDQRKRYIIGPITVSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFN
                     RYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAG
                     RMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            986..1099
                     /gene="yhiY"
                     /locus_tag="ACHK49_004327"
     CDS             986..1099
                     /gene="yhiY"
                     /locus_tag="ACHK49_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO9264852.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1162..2229
                     /gene="yhiI"
                     /locus_tag="ACHK49_004328"
     CDS             1162..2229
                     /gene="yhiI"
                     /locus_tag="ACHK49_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO9264853.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSESRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2226..4961
                     /gene="rbbA"
                     /locus_tag="ACHK49_004329"
     CDS             2226..4961
                     /gene="rbbA"
                     /locus_tag="ACHK49_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO9264854.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDSLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDITHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4961..6085
                     /gene="yhhJ"
                     /locus_tag="ACHK49_004330"
     CDS             4961..6085
                     /gene="yhhJ"
                     /locus_tag="ACHK49_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9264855.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
BASE COUNT         1437 a         1575 c         1773 g         1476 t
ORIGIN      
        1 gccttatttg gttattaata aagacgcagt cacgccatgt acgtttctgg gcgaccagac
       61 tggcgatcga tttagtaata tctgtggcga taaatttatc atcgatttat taaaacgcat
      121 aatgagtatc aatgaaaacg ttcatgtatt agctggaaat catgaaacga attgcaatgg
      181 taactatatg cagaacttca cgcgcatgaa accacttgat gaggatacct acgctgggat
      241 aaaagattat cctgtatgtt tctatggccc caaatacaaa ataatggcaa atcatcatgg
      301 gataactttt gatgatcagc gaaaacgtta cattatagga ccgataacag tctctagtga
      361 tgaaatgacc aatgctctcg atccggtcga attagcggca attatcaata aaaagcacca
      421 tgctgtaatt aatggcaaaa agtttaaaaa tagccgggct atctcttgtc gatcctttaa
      481 tcgttatttt tctgtttcga cagattacag acctaaactt gaagcactat tagcatgctc
      541 acaaatgtta ggtataaatc aagttgttgc acataacggt aatggtggaa gagaatgtat
      601 aggtgaaacg gggacagttt taggtcttaa cgcccgtgac agtaaacatg ctggaagaat
      661 gttcagcatg cataattgcc aaattaaccc tggtgcaggg cctgagataa ccactccctg
      721 gaagtcttac cagcatgaaa aaaacagaaa tggacttatg cctttgatta gaaggcgcac
      781 aatgttacag ctctgaacca tagacaacat gaatattaat gttactatct ggttgagact
      841 gttgataaaa cgtaaaaagg aatgctctgt tcctgcaact gaacaggcgt gaaacgcgat
      901 ccaacaggat ggataccgtt ttgtcagcca gttttataga tcacacttat gagtatgcgt
      961 gtttcattta cggccaggaa tgctgatgat gacaacatta ctacccgttt tcactaagcc
     1021 ttccccgttg gcgctcaatg ctctgcgcgc tggtcgtatt tgtcgtttcc ttcttatccc
     1081 ggatggaaga atccgctgaa cgtcgttcgg cggcagttgt aacacgcgtc gtttgcttac
     1141 agggattatg gcgggccgat tatggataag agtaagcgcc atctggcgtg gtgggttgtc
     1201 ggggcactgg cggtggctgc cgtcgtggcg tggtggctgt tgcgcccggc gggtgtgccg
     1261 gaaggctttg ctgtcagcaa tgggcgcatt gaagcgacgg aagtggatat cgccagcaaa
     1321 attgccgggc gtatcgacac cattctggtg aaagaaggcc agtttgttcg cgaaggtgaa
     1381 gtgctggcga agatggatac tcgcgtgttg caggaacagc gactggaagc catcgcgcaa
     1441 atcaaagagg cacaaagcgc cgttgccgcc gcgcaggctt tgctggagca acgacaaagc
     1501 gaaagtcgcg ccgcgcagtc gctggttaat caacgccagg cagaactgga ctccgtagca
     1561 aaacgtcata tgcgttcccg ttcactggcc caacgagggg ctatttctgc gcaacagctg
     1621 gatgacgacc gcgccgccgc tgagagcgcc cgagctgcgc tggaatcggc aaaagctcag
     1681 gtttctgctt ctaaagccgc tatagaggcg gcacgtacca atatcattca ggcgcaaacc
     1741 cgcgtcgaag cggcacaagc cactgaacgg cgcattgccg cagatatcga tgacagcgaa
     1801 ctgaaagccc cgcgtgacgg acgcgtgcag tatcgggttg ccgagccagg ggaagtgctg
     1861 gcggcaggcg gtcgggtgct gaatatggtc gatctcagcg acgtctatat gaccttcttc
     1921 ctgccaaccg aacaggcggg cacgctgaaa ctgggtggtg aagcccggct gatcctcgat
     1981 gccgcgccag atctgcgtat tccggcgacc atcagttttg tcgccagtgt cgcccagttc
     2041 acgccaaaaa ccgtcgaaac cagcgatgaa cggctgaaac tgatgttccg cgtcaaagcg
     2101 cgtatcccac cggaattact ccagcagcat ctggaatatg tcaaaaccgg tttaccgggc
     2161 gtagcgtggg tgcgggtgaa tgaagaactt ccgtggcctg acgacctcgt ggtgaggttg
     2221 ccgcaatgac gcatctggaa ctggttcccg tcccgcctgt cgcgcaactg gcgggcgtga
     2281 gccagcatta tggaaaaacc gttgcgctga acaatattac tctcgatatt ccggcccgct
     2341 gtatggtcgg gctgattggt ccggatggcg tcgggaagtc gagcttattg tcgttgattt
     2401 ccggtgcccg cgtcattgag cagggcaatg tgatggtgct gggcggcgat atgcgcgacc
     2461 cgaagcatcg ccgcgacgtc tgcccgcgca tcgcctggat gccgcagggg ctgggcaaaa
     2521 atctctacca caccttgtcg gtgtatgaaa acgtcgattt tttcgctcgc ctgttcggtc
     2581 acgacaaagc ggagcgggaa gtgcgaatta atgagctgct gaccagcacc gggttagcac
     2641 cgtttcgcga tcgtccggca gggaaactct ccggcgggat gaagcaaaaa ctggggctgt
     2701 gctgtgcgtt aatccacgac ccggaactgt tgattcttga tgagccaaca acgggggttg
     2761 actcgctctc ccgcgcccag ttctgggatc tgatcgacag tattcgccag cggcagagca
     2821 atatgagcgt gctggttgcc accgcctata tggaagaggc cgaacgcttc gactggttgg
     2881 tggcgatgaa tgccggagaa gtgctggcaa ccggcagcgc cgaagagttg cggcagcaaa
     2941 cgcaaagcgc cacgctggaa gaagcattta taaatctgtt accgcaagca caacgccagg
     3001 cgcatcaggc ggtagtgatc ccaccgtatc aacctgaaaa cgcagagatt gccatcgaag
     3061 cgcgcgatct gaccatgcgt tttggttcct tcgttgccgt tgatcacgtc aatttccgca
     3121 ttccacgcgg ggagattttt ggattccttg gttcgaacgg ctgcggtaaa tccaccacca
     3181 tgaaaatgct taccggactg ctgcccgcca gcgaaggtga ggcgtggctg ttcgggcaac
     3241 cggttgatcc aaaagatatc gatacccgcc gtcgggtagg ctatatgtcg caggcgtttt
     3301 cgctctataa cgaactcacc gtgcggcaaa accttgagtt acatgcccgt ttgtttcaca
     3361 tcccggaagc ggaaattccc gcgcgagtgg ctgaaatgag cgagcgtttt aagctcaacg
     3421 acgttgaaga tgttctgccg gagtcattgc cgctcggcat tcgccagcgg ctttcgctgg
     3481 cggtggcggt gattcatcgc ccggagatgt taatcctcga tgaacctact tctggcgtcg
     3541 atccggtggc gagggatatg ttctggcagt tgatggtcga tctctcgcgc caggacaaag
     3601 tgactatctt catctccacc cactttatga acgaagcgga acgttgcgac cgcatctcac
     3661 tgatgcacgc cggaaaagtg cttgccagcg gtacaccgca ggaactggtt gagaaacgcg
     3721 gagccgccag tctggaagag gcatttatcg cctatttgca ggaagcggca gggcagagca
     3781 acgaagccga agcgccgccc gtggtacacg acatcaccca cgcgccgcgt cagggattta
     3841 gcctgcgccg tctgtttagc tacagccgcc gcgaagcact ggaactgcgc cgcgatccgg
     3901 tccgttcgac gctggcgctg atgggaacgg tgatcctgat gctgataatg ggttacggca
     3961 tcagtatgga tgtggaaaac ctgcgctttg cggtgctcga ccgcgaccag accgtcagta
     4021 gccaggcgtg gacgctcaat ctctccggtt cccgttactt tatcgaacag ccgccgctca
     4081 ccagttatga cgagcttgat cgtcggatgc gtgcgggcga tatcacggtg gcaattgaga
     4141 tccctcccaa tttcgggcgc gatatcgcgc gtggtacgcc tgtggaactc ggcgtctgga
     4201 ttgacggagc gatgcccagt cgtgccgaaa cggtaaaagg ttacgtgcag gcaatgcacc
     4261 agagctggtt acaggatgtg gcgagccgac aatccacacc cgccagccaa agcgggctga
     4321 tgaacattga gacgcgttat cgctataacc cggacgtaaa aagcctgcca gcgattgttc
     4381 cggcggtgat cccgcttctg ctgatgatga tcccgtcaat gctaagcgcc cttagcgtgg
     4441 tgcgggaaaa agagcttggg tcgattatca acctttacgt gacccccacc acgcgtagtg
     4501 aatttttgct tggtaaacag ttgccataca tcgcgctggg gatgctgaac tttttcctgc
     4561 tctgcggcct gtcggtgttt gtgtttggcg taccgcataa aggcagtttc ctgacgctca
     4621 ccctggcggc gctgctgtat atcatcattg ccaccggaat ggggctgctg atctccacct
     4681 ttatgaaaag ccagattgcc gccattttcg gaacggcgat tatcacgttg atcccggcga
     4741 cacagttttc cgggatgatc gatccggtag cttcgctgga agggcctgga cgttggatcg
     4801 gcgaggttta cccgaccagt cattttctga ctatcgcccg cgggacgttc tcgaaagcgc
     4861 tggatctgac tgatttgtgg caacttttta tcccgttgct gatagccatc ccgctggtga
     4921 tgggcttaag tatcctgctg ctgaaaaaac aggagggatg atgcgccatt tacgcaatat
     4981 ttttaatctg ggtatcaaag agttgcgcag tctgctcggt gataaagcga tgctgacgct
     5041 gattgtcttc tcgtttacgg tgtcggtgta ttcgtcagcg accgttacgc caggatcgtt
     5101 gaacctcgcg ccgatcgcca ttgccgatat ggatcaatcg cagttatcga accggatcgt
     5161 taacagcttc tatcgtccgt ggtttttgcc accggagatg atcaccgccg atgagatgga
     5221 tgccggactg gacgccggac gctatacctt cgcgataaat attccgccta attttcagcg
     5281 tgatgtcctc gccggacgcc agccagatat tcaggtgaac gtcgatgcca cgcgcatgag
     5341 ccaggcattt accggcaatg ggtatatcca gaatattatc aacggtgaag tgaacagctt
     5401 tgtcgcgcgc taccgtgata acagcgaacc gttggtatcg ctggaaaccc ggatgcgctt
     5461 taacccgaac cttgatcccg cgtggtttgg cggggtgatg gcaatcatca acaacattac
     5521 catgctggcg attgtattga ccggatcggc gctgatccgc gagcgtgaac acggcacggt
     5581 ggaacacttg ttggtgatgc cgataacgcc gtttgagatc atgatggcaa aggtctggtc
     5641 gatggggctg gtggtgctgg tggtgtcggg attatcactg gtgctgatgg tgaaaggcgt
     5701 gctgggtgta ccgattgaag gctcgatccc gctgtttatg ctgggcgtgg cgctcagtct
     5761 gtttgccacc acgtcaatcg gcatttttat ggggacgata gcgcgttcaa tgccgcaact
     5821 ggggctgctg gtgattctgg tgctgctgcc gctgcaaatg ctttccggtg gatccacgcc
     5881 gcgcgaaagt atgccgcaga tggtgcagga cattatgctg accatgccga cgacacactt
     5941 tgttagcctc gcgcaggcca tcctctaccg gggtgccgga ttcgaaatcg tctggccgca
     6001 gttcctgacg ctgatggcaa ttggcggcgc gtttttcacc attgcactgc tgcgattcag
     6061 gaagacgatt gggacaatgg cgtaaataca attgcccgat gcgtaagctt atagggacta
     6121 cgcatcggtg caatttattg aattttagtt gtttttaggc cggataaggc gtttatgaac
     6181 tgacccccaa aacctggaca gtttcagtta accggctttt tgatactcag tcctgtattc
     6241 agggcaagat tacgaaagcc c
//
LOCUS       DAZGLJ010000252         6186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000252 DAZGLJ010000000
VERSION     DAZGLJ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.252"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1055)
                     /locus_tag="ACHK49_004331"
     CDS             complement(<1..1055)
                     /locus_tag="ACHK49_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO9264856.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFDGTPGNHAAQFGALVDKFRADLTD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESSYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIG"
     gene            1299..1622
                     /locus_tag="ACHK49_004332"
     CDS             1299..1622
                     /locus_tag="ACHK49_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO9264857.1"
                     /translation="MNWIVIDTVIQPACDISFSAIWCNIKLILWYQSDIFLPPGCIFT
                     PTRTGVILNNKELPVTIYNVTPFNKNVWNLIKSSQECPANTGNITDKCFNHRCILEIC
                     PYGRK"
     gene            complement(1920..2189)
                     /locus_tag="ACHK49_004333"
     CDS             complement(1920..2189)
                     /locus_tag="ACHK49_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFO9264858.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(2201..3160)
                     /locus_tag="ACHK49_004334"
     CDS             complement(2201..3160)
                     /locus_tag="ACHK49_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO9264859.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            complement(3251..3327)
                     /locus_tag="ACHK49_004335"
     tRNA            complement(3251..3327)
                     /locus_tag="ACHK49_004335"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3291..3293),aa:Arg,seq:tct)
     gene            complement(3341..3417)
                     /locus_tag="ACHK49_004336"
     tRNA            complement(3341..3417)
                     /locus_tag="ACHK49_004336"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3381..3383),aa:Arg,seq:tcg)
     gene            complement(3427..3502)
                     /locus_tag="ACHK49_004337"
     tRNA            complement(3427..3502)
                     /locus_tag="ACHK49_004337"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3467..3469),aa:Ile,seq:cat)
     gene            complement(3543..4601)
                     /locus_tag="ACHK49_004338"
     CDS             complement(3543..4601)
                     /locus_tag="ACHK49_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO9264860.1"
                     /translation="MFNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWTGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYLKLQSLFARVA
                     EEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAALALGRRATGVELET
                     ERFEQTVREVQDLASQNG"
     gene            complement(4753..4950)
                     /locus_tag="ACHK49_004339"
     CDS             complement(4753..4950)
                     /locus_tag="ACHK49_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO9264861.1"
                     /translation="MLKQQDMTETARVVFNELSATAPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(5166..5546)
                     /locus_tag="ACHK49_004340"
     CDS             complement(5166..5546)
                     /locus_tag="ACHK49_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO9264862.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSTTKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            complement(5565..>6186)
                     /locus_tag="ACHK49_004341"
     CDS             complement(5565..>6186)
                     /locus_tag="ACHK49_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9264863.1"
                     /translation="LRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVVTMPELCW
                     WQVRNDLADALPESAARKALRLPKPVLPSVTRESDLVPSVPATSIIQDKAKKVLALKV
                     DPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKAHD
                     LFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
BASE COUNT         1593 a         1710 c         1338 g         1545 t
ORIGIN      
        1 ccgatacccg gaatgtccgg gtcttcttcc ggtttgttcg tcggcacatt caccccgttc
       61 tcatcggtca tgaacggtac gaaatggata ttcttttccg ttttgttttt gtagctgccg
      121 tacaccgtct ggtacgagga ttcgttcttc tgcttccaga aatacgtcgt atctccacag
      181 atccagggaa caccgccagc agagccacca acgcactgac ctgccatatc cgtcaggtct
      241 gcacggaatt tatcaaccag cgcaccaaac tgtgctgcgt gatttccggg cgtgccgtca
      301 aaatcaaatt ccccctgcat ccacaccacg gcaaacagca catttttcgg gttcttcttc
      361 agtgctgctt ttgttcgacc gataaggtcc ttatacagcg gcttgtccac accccagcgg
      421 gttgaattct ccgaggcacc actcgcgtca ctgtatgtgc catcagctcc ggtggtgaac
      481 gctgaaccac cacgacagca cggaaccagc agaatgcccg cattcgccgg tataaacggc
      541 agcagttttt tggcgatatg cagcccctgc cccacggtac cgtactgccc ttttgacagg
      601 tccgctttcg gatggttaag acggctcatg tcctgcacat catgcagaca atggtccgcc
      661 ggaatgatgt cgttatattt gcatgctgca ccgcccggtg tcaccgtact gcggcgcgcc
      721 agctgcttaa tacgcgggtc cggacggtca tatgtctccg gcagcggaag accttcacca
      781 tacgacatgc catttgactg ccctgccaga accacaacaa agtaatactc cgggtcgctg
      841 gtggcactga cagccaccgc cttaccctca ttaccggtca ccgccactgg tgtggtgaca
      901 tcaccttccg ccgcaatggc ctgcatcagg gtataaggcg tgatggccac cggactgcca
      961 aatggctgcc agccctcctt cagtttttgt gtcagtcgtt cagcaaggtc tgacggcgat
     1021 gccgccctga ccacatcgta gtgtttaaat gtcataacct ctcctgccgc cagcaaagta
     1081 attatccgga atacacaatc atgtaacaca taaaaaacac gctataaaaa aaccatgtct
     1141 cttatataac aaaaaaacgc cgacaattta aaatgtcctc ttattttaaa caaagaataa
     1201 acgacaccag aaaaacaact caggctgaca gattgctttc aaaacatcac ttataattta
     1261 tggcatcatt taaaacctta agaaaataag gaaagcaaat gaactggata gtgattgata
     1321 cggtaatcca gccagcatgt gacatatcat tttcagccat atggtgtaac ataaaattaa
     1381 tattatggta tcagtctgat atcttcctcc ctccgggctg tatttttaca ccaacccgga
     1441 caggcgtaat actcaataat aaagagctcc ccgtcaccat ttacaacgtg accccattta
     1501 ataaaaacgt ctggaattta attaaaagca gccaggaatg ccctgcaaac acaggaaata
     1561 tcacagataa gtgttttaat catcgctgca ttctggaaat atgcccatac gggcgaaaat
     1621 aacagaatca ttcagataaa aagcccctcc ggagaggggc tgaagccgca tttctgtatc
     1681 accatgaaca tggtgccggg tgcctcccgg tgagttcagt ccggtgtccc tgaacccgcg
     1741 tatctcgatc caggttgtcg tcagagatga caccttatac accagtcgcc cctccgcaca
     1801 gggggcttca ccatgcgaaa tttttttaac aaatgctcag tctgacaggc aactgtcaac
     1861 tgactgaatt gtgacacaga ttacacttgt tacccacata ccacgaatca ggttatgcct
     1921 cagtcattat taaactgcac ttcagcaaat ccggagcctg attcacaggt actggatttg
     1981 attgtgacag tcattcctgt caactgagca ctttgcagta acggttgcag attccagcga
     2041 ctggtccagt attctttccc gtcaaccttc actgtaaatg tgtcatcctc attatacttg
     2101 gaaaactcaa ttttaccttt agcacaatcc gccgccattg cattaacaga aactaatgca
     2161 aataaaaccg ccataaacat cttcttcata cttaactcct ttatttaccc gttgtatata
     2221 aaaactgtga ctttctgttc agaaacgctg cagctgtatt actttcccat aatgtattgt
     2281 ttatttttat aacgggcctg tcgccagtta tctgacattc tggttgactc tcttcattca
     2341 cggcgcgaac agaacgcgcc ccctgatgat ggcaattcag tataacggcc acagtaccca
     2401 gtatcgctga tatattatta aaggatattc tccccactct gacaccatcc tctccccgat
     2461 actccggaag cacattgctg attcgccccc agttcagagt gaggtccacg tctcccggcg
     2521 tcatcgtata cacaggagca gtttcagaca gtgcctgacg aaattctctc tgtatctgcc
     2581 tgaagcgtaa ggcttctgcc gtgacagtga caaaacgcag aactgctctg gatgcatctc
     2641 tggtcattgt attaccactg aactccatta acgccagata tgatgaaacc agtgagtgac
     2701 gactgatttg cattccggaa cgttccagcg ctgcgacacg ttgcagagtg gtataactgc
     2761 tgtccgttgt catggaaacc gttgtcacat cgggcactga tatatgtgta aaatctgaaa
     2821 aacggtagaa agtatttgtt gccgtattaa cgaacccggc cacatataaa ttattttgct
     2881 caataatcag acgaagatgg tcaaaacgcg cctgatagac atcaagccct cgtatatcca
     2941 cagcaaaata actgcccggc ggggtgtggt taataacaga caccgatgtg gtcccctgag
     3001 atatatgttc aagaggggtc gatatctctg tccgtatact atttaacgaa gagacataac
     3061 tttgttgagt cgaaaagtct atcgtaaatt cccgggaata ggataccgaa gaaaaaccca
     3121 gtaacaggca cagtacccat ttaaataata tacacttcat atacaggtgt tccttttggc
     3181 tgaagtaatc agcaccagac ccggcgcaga tataaaaaag gcccgcaaaa gcgagcctgg
     3241 taaataaata tggcgcgttg tactggattc gaaccagtga ccgattgctt agaaggcaat
     3301 tgctctgtcc ggctgagcta acaacgctga ataccgataa tggaccgcca tcgaggactc
     3361 gaaccccgcg cagccagctt cgaaggctgg cgctctgtcc cgatgagcta atggcggtat
     3421 gtgatatggt ggcccttgct ggatttgaac cagcgacctg gcgattatga gtcgctcgct
     3481 ctcaccactg agctaaaggg ccgggcgcag gataataacg gtacgtaact aatcctgcaa
     3541 tatcatccgt tctgactggc taaatcctga acttccctga ccgtctgctc aaaacgttca
     3601 gtctccagct caacgccagt tgcacgacgc cccagcgcca gtgcagcttt gactgtcgaa
     3661 cccgacccca tgaaaaaatc tgcaaccagg tcacccggac gactgctcgc gctgattatc
     3721 tgctgcagca tttctgccgg tttttcgcac ggatgtttcc cgggatagta ctgcaccggt
     3781 ttatgcgtcc acacatccgt gtacggcacc tgcgccgtca cgccaaaata ccgccgcaga
     3841 tgcttatatt cactctgcag ttccgcatac tgccggttca gtgaagtata cgtctccagc
     3901 agctggtggt ggggcttttc cagttcaccg cgctgatgct tctcttctgc cacccgggca
     3961 aacagcgact gtaatttcag ataatcgctt tcgttcggta gctgccactg actggcactg
     4021 aaccagtgcg acaccatgtt tttctttcct gtggcatcca ctatctgttt tgccgttatc
     4081 cccagggcag cacgcgcatc acgaaagtaa gcaatcagcg gagccatcac atgctgtttc
     4141 agtgccctgc ccttcgcctc atacccggca tctttcggac gatacggccc ctgataatgt
     4201 tccgcgaaca gaatgcgctc tgtggcgggg aaatacgccc tcaggctttc cttgttgcac
     4261 ccgttccagc gtccggacgg cttcgcccag ataatatggt tcagcacact gaagcgttca
     4321 cgcatcatga tttcaatgtc agatgccagg cgatggccac agaacaggta aagacttccg
     4381 gcaggtttca gcacccgcca gaactgcgcc agacactggt ccagccattt caggtaatcc
     4441 tcatcacccg tccactggtt atcccagccc tcaggcttca ctttaaagta cggcgggtcc
     4501 gtgactatca ggtcaacaga attttcgggt aacgaccgga taaattccag gcagtcggcg
     4561 ttgattaact cacaactgga tatttttaca gtattaaaca tggatcatta agcctgtctc
     4621 tgataggctc ataccgcttt tgcgcaaagc agatgggcct gaggtttgct tgtgacccca
     4681 acgcatgagc agatggctgg tgagtgcccc taacacccac cagccgccca tttaccacaa
     4741 ataaaaaagc cttcaggact gaaggcgtct gtaacaacca aactgatagt ctgccagacc
     4801 cgccataaca agctgggtca gtattagctg gcagcgttcg cgtgaaaggt acgtattctg
     4861 tgcaatctcc ccgactgtcg ccggtgcggt ggcacttaat tcattaaaca ccactctggc
     4921 ggtttctgtc atatcctgct gtttcagcat gtctttttcc cttttccggt taacgtgaca
     4981 caccaataac tcttgtcgaa aaagccagca agctgaaaga ccggtattca ccgccaccag
     5041 cgcgtttaac gtactggtcc gatttcaggc ataaaaaacc cgcctgacgg cggatttaag
     5101 ctgtgtggca aagtaaccac tcttaacata ctgacatact ttttgcggac cgcactaatc
     5161 attttttact tttttagcag ccagtcgtcc atctccagtc ttacccccag cacagacaaa
     5221 catccgtcaa taaacccttc ggctatctgc atctcaattc gtattgcttt ttcgcttttc
     5281 tttctcgtcc tggctatctg tctttttgat attcgcaaca aataatgagc aatgagaagc
     5341 gaatactcct caggtttttt ctgcttcaga cgtgcaagac agttttcaat gataagtccg
     5401 tcatcatcgc agcaggctgg acgtggttta gtggtagatg gtaaaaggcc tttgaatccg
     5461 gcagcgatcg gagaatagtc caccccggtg ttaccacttg ccgcccatgc cccccagcgt
     5521 tcaagaacca tctgaatatc acgcatcaac tttctccaca aaaatcagga cagcacacca
     5581 atcgccagtg cgcgatcgat aaaacgaaat atcagctcca gttgggaacc atacttctct
     5641 tcaaatgcca cggtatccgc atgcagttcg tcatggtgtt ttctgcacaa aggcaacaca
     5701 aaaaggtcat gcgcttttgt acccattcca ccctgaccat ggccaatcag gtgatgtgga
     5761 tcgtcggctg gcttaccaca acacgcgcaa ggctgtgtct taacccagcg cgtgtacttt
     5821 tcattaaccc agcgacgacg tttggggcgt aacataaaag actccggcga ctccggatcc
     5881 actttcagcg ccagcacctt tttcgcttta tcctggataa tgctggtggc aggaaccgaa
     5941 ggcacaaggt cactctcccg ggtgacagac ggcaaaacag gcttcggtaa tctcagggcc
     6001 ttacgggctg cactttccgg taaggcatcc gccaggtcat tacgaacctg ccaccagcac
     6061 agttcaggca ttgtcacaac gtgactgtca tcaaaaccga gatcacgacg gactacggac
     6121 aacacccagc gggcacagtt atccgttgcc attgactcca gacgttccgt gaactgatcg
     6181 cgaagc
//
LOCUS       DAZGLJ010000253         6174 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000253 DAZGLJ010000000
VERSION     DAZGLJ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.253"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(587..1063)
                     /locus_tag="ACHK49_004342"
     CDS             complement(587..1063)
                     /locus_tag="ACHK49_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO9264864.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1122..2411)
                     /gene="bioA"
                     /locus_tag="ACHK49_004343"
     CDS             complement(1122..2411)
                     /gene="bioA"
                     /locus_tag="ACHK49_004343"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO9264865.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            2498..3538
                     /gene="bioB"
                     /locus_tag="ACHK49_004344"
     CDS             2498..3538
                     /gene="bioB"
                     /locus_tag="ACHK49_004344"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO9264866.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            3535..4689
                     /gene="bioF"
                     /locus_tag="ACHK49_004345"
     CDS             3535..4689
                     /gene="bioF"
                     /locus_tag="ACHK49_004345"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO9264867.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDIAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            4676..5431
                     /gene="bioC"
                     /locus_tag="ACHK49_004346"
     CDS             4676..5431
                     /gene="bioC"
                     /locus_tag="ACHK49_004346"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO9264868.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            5424..6101
                     /gene="bioD"
                     /locus_tag="ACHK49_004347"
     CDS             5424..6101
                     /gene="bioD"
                     /locus_tag="ACHK49_004347"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO9264869.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         1465 a         1586 c         1693 g         1430 t
ORIGIN      
        1 tctgttttta ttagagaata tgatgttttt tcgttgcgta atatgttact aattgatgat
       61 aagcagttct ttatgattgg cataataaat tccttgtctt catatggatg aacttttgat
      121 aggaagtctt atattatata aactaattgc tatcattttt gtagttaatt gtctagtaga
      181 gttgtcattt tttatgttct attcttattg attctgttta ggcatagcca taatgcatga
      241 tggtgtggcg tggcaaagca gagatataca aagtctgcca catgttttta tgacaaaaaa
      301 ttagcgcaag aagacataaa atcaccttgc gctaatgctc tgtctcaggt cactaatact
      361 atctaagtag ttgattcata gttactagat atgttgtgtt ttgtagtatt atgcagtcta
      421 ttttttagac taaatatatt gtaacacatt gatattaatg gtctttagcg tttcgcgttc
      481 agctttttta tactaacttg agcgaaacgg gaaggtaaaa agacaaaaag ttgtttttaa
      541 tacctttaag tgataccaga tggcattgcg ccatctggca gagtgattaa ctaaacatcg
      601 cagtaatcga ggcgcttgcc agagagtgga aatgaacgtt aaacccgacc atcgcgccgc
      661 tggcaccttc atcgacatca atacgttcta tatccagcgc gtgaacggta aaaatgtagc
      721 gatgagtttc gcctttcggc ggtgctgcgc catcgtaccc ggttttacca aagtcggtac
      781 gcgtctgcaa aacgccgtct ggcattgcta ccagaccaga gccaaaccct tgcggtaata
      841 cgcgggtatc agcgggtaag ttaacaacta cccagtgcca ccagccggag ccggttggcg
      901 catccgggtc gtagcaggtg acaacaaaac ttttcgttcc cgcaggaaca tcatcccacg
      961 ccagatgcgg tgaaatatta tcgccatcgt aacccatgcc gttaaagaca tgacgatgcg
     1021 gcaatttatc gccatcgcgc agatcgttac tgatgagttt catgaaccct cctttcttgt
     1081 ttgcagaaag tgtagccaga aaccctcacg cggacttctc gttattggca aaaaaatgtt
     1141 tcatcctgta ccgcgcggtt aaccgctgcg gtcagacgct gcaactgttg cgggagaata
     1201 atatagggcg gcatcaggta aatcagtttg ccaaaaggcc ggatccagac accctgttcg
     1261 acaaagaatt tttgcagcgc cgccatattc accggatgag tggtttcgac tacgccgatt
     1321 gcccccagca cgcgcacatc ggcaaccatt tcggcatcac gggcgggggc aagttgctcg
     1381 cgcagctgta cttcaatagc cgccacctgt tgctgccagt cgccagattc gagaatcgcc
     1441 aggctggcgt ttgctgccgc gcaggccagc ggattgccca taaaagttgg cccatgcata
     1501 aagcaaccgg cttcgccgtt actgatggtt tccgcaacct cgcgcgtggt gagcgtggcg
     1561 gaaagggtca ttgtgccgcc ggttaaggct ttaccgaggc acaaaatgtc cggcgcgatt
     1621 tctgcatgtt cacaggcaaa cagtttgccg gtacgaccaa atccggtggc gatctcgtcg
     1681 gcaatcagca agataccttc gcgatcgcac attttgcgga ttcgttttaa ccattccgga
     1741 tggtacatgc gcatcccgcc tgcgccctgg acaatcggtt caatgatcac cgccgcgatt
     1801 tcatgacgat gcgccgccat caggcgggca aagccaacca tatcacgttc atcccattca
     1861 ccatccatgc ggctttgcgg ggcgggagca aacaagtttt ctggcagata gcctttccac
     1921 aggctgtgca ttgagttgtc cggatcgcac accgacatcg cgccaaaggt atcgccatga
     1981 taaccattgc ggaaggtcag aaaacgctgg cgcgcttcgc ctttggcctg ccagtactgc
     2041 aacgccattt tcatcgccac ttccaccgct acggagccgg agtccgcgag aaaaacgcat
     2101 tccagcggtt gcggcgtcat tgccaccagt ttgcggcata gctcgatggc gggcgcatgg
     2161 gtgataccgc caaacatcac atgcgacata gcatcaattt gcgacttcat cgccgcatta
     2221 agctgcgggt gattatagcc gtggatcgcc gcccaccagg acgacatacc gtcaaccagg
     2281 cgtctgccgt cagacaaaat tagctcgcaa ccttcggcgc tcgcgaccgg ataaaccggc
     2341 agaggggagg tcatggatgt gtatgggtgc cagatatggc gttggtcaaa ggcaagatcg
     2401 tccgttgtca taatcgactt gtaaaccaaa ttgaaaagat ttaggtttac aattctacac
     2461 cgaattaaca acaaaaaaca cgttttggag aagccccatg gctcaccgcc cacgctggac
     2521 attgtcgcaa gtcacagaat tatttgaaaa accgttgctg gatctgctgt ttgaagcgca
     2581 gcaggtgcat cgacagcatt tcgatcctcg tcaggtgcag gtcagcactt tgctgtcgat
     2641 taagaccgga gcttgtccgg aagattgcaa atactgcccg caaagctcgc gctacaaaac
     2701 cgggctggaa gccgagcggt tgatggaagt tgaacaggtg ctggagtcgg cgcgcaaagc
     2761 gaaagctgcg ggatcgacgc gtttctgtat gggcgcggcg tggaagaatc cccacgaacg
     2821 cgatatgccg tacctggaac aaatggtgca gggggtaaaa gcgatggggc tggaggcgtg
     2881 tatgacgctg ggcacgttga gtgaatctca ggcgcagcgc cttgcgaatg ccgggctgga
     2941 ttactacaac cacaacctcg acacctcgcc ggagttttac ggcaatatca tcaccacccg
     3001 cacttatcag gaacgcctcg atacgctgga aaaagtgcgc gatgccggga tcaaagtctg
     3061 ttctggcggc attgtgggct taggcgaaac ggtaaaagat cgcgccggat tattgctgca
     3121 actggcaaac ctgccgacgc cgccggaaag cgtaccaatc aacatgctgg tgaaggtgaa
     3181 aggcacgccg ctggcggata acgatgatgt cgatgccttt gattttattc gcaccattgc
     3241 ggtcgcgcgg atcatgatgc cgacctctta cgtgcgcctt tctgccggac gcgagcagat
     3301 gaacgaacag actcaggcga tgtgctttat ggcaggcgca aactcgattt tctacggttg
     3361 caaactgctg accacgccga atccggaaga agataaagac ctgcaactgt tccgcaaact
     3421 ggggctaaat ccgcagcaaa ctgccgtgct ggcaggggat aacgaacaac agcaacgtct
     3481 tgaacaggcg ctgatgaccc cggacaccga cgaatattac aacgcggcag cattatgagc
     3541 tggcaggaga aaatcaacgc ggcgctcgat gcgcggcgtg ctgccgatgc cctgcgtcgc
     3601 cgttatccag tggcgcaagg agccggacgc tggctggtgg cggatgatcg ccagtatctg
     3661 aacttttcca gtaacgatta tttaggttta agccatcatc cgcaaattat ccgtgcctgg
     3721 cagcagggtg cggagcaatt tggcgtcggt agcggcggtt ccggtcacgt cagcggttat
     3781 agcgttgcgc atcaggcgct ggaagaagaa ctggccgagt ggctgggcta ttcgcgggca
     3841 ctgctattta tctctggttt tgccgctaat caggcggtca ttgccgcgat gatggcaaaa
     3901 gaggaccgca ttgtcgctga ccggcttagc catgcctcat tgctggaggc tgccagttta
     3961 agcccgtcgc agcttcgccg ttttgttcat aacgatgtca ctcatctggc gcgactgctt
     4021 gcttccccct gtccggggca gcaactggtg gtgacagaag gcgtgttcag catggacggc
     4081 gatattgcgc cactggcgga aatccagcag gtaacgcaac agcacaatgg ctggttgatg
     4141 gtcgatgatg cccacggcac gggcgttatc ggggagcagg ggcgcggcag ctgctggctg
     4201 caaaaggtaa aaccagaatt gctggtggtg acttttggca aaggatttgg cgtcagtggg
     4261 gctgcggtgc tttgctccag tacggtggcg gattatctgc tgcaattcgc ccgccacctt
     4321 atctacagca ccagtatgcc gcccgctcag gcgcaggcat tacgtgcgtc actggcggtc
     4381 attcgccgtg atgaggggga tgcacggcgc gaaaaactgg tgtcactcat tgcgcgtttt
     4441 cgtgccggag tacaggattt gccgtttacg ctggctgatt catgcagcgc catccagcca
     4501 ttgatcgtcg gtgataacag ccgtgcgtta caactggcag aaaaactgcg ccagcaaggc
     4561 tgctgggtca cggcgattcg cccgccaacc gtacccgctg gtactgcgcg actgcgctta
     4621 acgctaaccg cagcgcatga aatgcaggat atcgaccgtc tgctggaggt gctgcatggc
     4681 aacggttaat aaacaagcca ttgcagcggc atttggtcgg gcagccgcac tctatgagca
     4741 acacgcagat ctacagcgcc agagtgctga cgtcttactg gcaatgctcc cacagcgtaa
     4801 atacacccac gtactggacg cgggttgtgg acctggctgg atgagccgcc actggcggga
     4861 acgtcacgcg caggtgacgg ccttagatct ctcgccgcca atgcttgttc aggcacgcca
     4921 gaaggatgcc gcagaccatt atctggcggg agatatcgaa tccctgccgt tagcgactgc
     4981 gacgttcgat cttgcatgga gcaatctcgc agtgcagtgg tgcggtaatt tatccacggc
     5041 actccgcgag ctgtatcggg tggtgcgccc cggaggcgtg gtggcgttta ccacgctggt
     5101 gcagggatcg ttacccgaac tgcatcaggc gtggcaggcg gtggacgagc gtccgcatgc
     5161 taatcgcttt ttaccgccag atgaaatcga acagtcgctg aacggcgtgc attatcaaca
     5221 tcatattcag cccatcacac tatggtttga tgatgcgctc agtgccatgc gttcgctgaa
     5281 aggcatcggt gctacgcatc ttcatgaagg gcgcgacccg cgaatattaa cgcgttcgca
     5341 attgcagcga ttgcaactgg cctggccgca acaacagggg cgatatcctc tgacgtatca
     5401 tctttttttg ggagtgattg ctcgtgagta aacgttattt tgtcaccgga acggataccg
     5461 aagtggggaa aaccgtcgcc agttgtgcac ttttacaagc cgcgaaggca gtaggttatc
     5521 ggacggcagg ttataaaccg gtcgcctctg gcagcgaaaa gaccccggaa ggcttacgta
     5581 atagtgacgc gctggcgtta cagcacaaca gcagcctgca gctggattac gcaacggtaa
     5641 atccttacac cttcgcagaa cccacctcgc cgcacatcat cagcgcgcaa gagggcaggc
     5701 cgatagaatc attggtaatg agcgccggat tacgcgcgct tgaacaacag gctgactggg
     5761 tgttagtgga aggtgctggc ggctggttta cgccgctttc tgacactttc acttttgcag
     5821 attgggtaac acaggaacaa ctgccggtga tactggtagt tggtgtgaaa ctcggctgta
     5881 ttaatcacgc gatgttgact gcgcaggcaa tacaacacgc cggactgact ctggcgggtt
     5941 gggtggcgaa cgatgttacg cctccgggaa aacgtcacgc tgaatatatg accacgctca
     6001 cccgcatgat tcccgcgccg ctgctgggag agatcccctg gcttgcagaa aatccagaaa
     6061 atgcggcaac cggaaagtac ataaaccttg ccttgttgta gctatcctgt atttggttaa
     6121 attgcgagcg agatcgcgtc ttcgattgac tgcaatttaa ccaattaaat tcta
//
LOCUS       DAZGLJ010000254         6118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000254 DAZGLJ010000000
VERSION     DAZGLJ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.254"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(99..438)
                     /locus_tag="ACHK49_004348"
                     /pseudo
     CDS             complement(99..438)
                     /locus_tag="ACHK49_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(754..1398)
                     /locus_tag="ACHK49_004349"
     CDS             complement(754..1398)
                     /locus_tag="ACHK49_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264870.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERGWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            complement(1419..1688)
                     /locus_tag="ACHK49_004350"
     CDS             complement(1419..1688)
                     /locus_tag="ACHK49_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO9264871.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            complement(1767..2405)
                     /locus_tag="ACHK49_004351"
     CDS             complement(1767..2405)
                     /locus_tag="ACHK49_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFO9264872.1"
                     /translation="MGDSVTPEILGNMIRQYFSQERPEEEAIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            complement(2390..3613)
                     /locus_tag="ACHK49_004352"
                     /pseudo
     CDS             complement(2390..3613)
                     /locus_tag="ACHK49_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            complement(4114..>6118)
                     /locus_tag="ACHK49_004353"
     CDS             complement(4114..>6118)
                     /locus_tag="ACHK49_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO9264873.1"
                     /translation="SNNNSAVSNVYEKMDKQQLGYVVARLGSTENRQQFFSTSISRSE
                     EVLPDSPSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEE
                     LFSHKYRLSSRKTTDLMAYIHYLSDRRIGFRNRIDLLLNFRILKVKPLVIPERRLALF
                     TSLQLSYYEKFIREKQVSLNEYEEVLKESDFKILLERLTSWSVLYLKQHLRRNISTRN
                     SFSAETYRDEFNRFIKRFPVIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGL
                     GCARNVIVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLK
                     EHYRCHPKIIQFCNKQFYDNALIPLTLDSGEASLSLIITAKGNHTRNFSNLRELESLE
                     GHYWDEESSRGYIAPYNAQVNLAETVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRS
                     SQHSRNIAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGE
                     IFESPVISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMM
                     FHSQIALNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTS
                     VQAERDELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
BASE COUNT         1653 a         1559 c         1214 g         1692 t
ORIGIN      
        1 cagaagcaga aaaagcttat tatgcttcca tcggaaacga tgatctggca gcctgagttc
       61 acagataaaa cactctccag gaaacccggg gcggttcaaa cctgattctg gaaaaggact
      121 ttgtggattg ttgctattgc cagtgagatt gaatgaagtc gttttaactc gctttgaata
      181 cgtcttgccc ccagatttcg ctgagaacgt aactccagaa tcagagcgac ttcgcaagtg
      241 ccggttttcg ttgatggtga acgcttgggg cgacggctgt ggctttccag accagcgatg
      301 ccctgagcca gatatctgcg ccaccatttt acgcaatgtg ggccgagaaa tgccacagcg
      361 acggcataca aatcccgcat caccggacgt ttcatagagt tttacccact gtagccgctg
      421 ctgaatttcc ctgttcataa tttggcatta gttcagctca atcccgactt aaaaagtccc
      481 tacaggatgt tccggacctg aggcacagtt catatcctta tccataaaaa atttagctat
      541 gtctatagca ttaatcttga caagacgacg ttagggcctg gcgatgaatc atttcccctg
      601 ccagatatcc tttggggtgc accagaacta ccgttttata tctatataac tgaaacgtcc
      661 ctcgccatcc ttcatatttt gatgtagtaa caccattcac tttcatctac cccccgtttt
      721 attaacatat agtaaaaatc acctcgccgc attttattct tttgaagtaa aatcatttac
      781 cagttcactt aaagctctct tttgttcatc atcatacaga gattgaatcc ggaatttaat
      841 ctcttctgca accagtcttg catcgggctc ataaaatatt tttgttattc gctgtttctg
      901 atgttcaggc tggtcttcca tatttcgtaa aaccagatga aataatatat cataaagtgc
      961 gcaatgccat gaataaccat cattggttaa aataagctcc caaccccgct cctgctcggc
     1021 aataagctgc agaacaaagg actcaacctg ttctgtttgt tcgggggcaa cacggtatag
     1081 cggagcaatc acagcaaaag atagttcatt taaccaggat tccgcttcct tctcgctcct
     1141 gaacggtgga cagttttgta ttcttcgttc gtattcacgg gtaatctgct ccagaatatc
     1201 ctccggcggt gtcagccact tattcctgag caatacactg gttgatggat gacagctggc
     1261 tatttcagca ataatttcac gtaatgtctc cgggctgaac ttcttcagct gattgtagtg
     1321 ctcccgggta aaaccatgtt catcgcataa aagttgccat gtcattgata tactgtttgc
     1381 cattccttgc ttcatcatta atacctgtta cctgaatatc aacagaccgg tgtacctttt
     1441 ctgaatacct ccccggacgg agtggcaatc cccattcgcc tcagcgtggc atctgcagct
     1501 ttcgccacct cgctgatatc cagcgtttca ggtagcggcg gggacgatat ctgcgccaga
     1561 caataattcc ggcgcaacaa caaccactcc cgttgtgctt ctgtacattc aacatttccc
     1621 ataacaaaaa gccagcggtc agaagctctg cgccagcaac cagcagcctc cagtttctgc
     1681 gcaaccatgt cttccacaac ttccggggta ctctttttcc tgctcaaaac attaactcac
     1741 tgaaataata tacatatctg acggtctcag cgaaccgtcc atgccgggct gaaatcctct
     1801 tcctgaaaca gctccgtcag cccgctaatc tgtttcaatc tgagaacttc atcctggtcc
     1861 atacccagct ccgtaccaat gcgttcattc gtccagccca ggcgcgacag gtccctgaca
     1921 atatcagaca ttgaggttat ctgatgtttg cccctggccc ggttatgtcg gatggtcgca
     1981 gcaatacgtg aggcctgtcc ctttctctcc ggattaatac agaccaccgg cagccagccc
     2041 ttcagacgtt ttcccgtacc tgcttccctg cccagtacct ggcgatgaaa accatccacc
     2101 accaagtaat ggttcttttc ctcagacact accaccggtt gtgtgaaacc atctttctcc
     2161 agtgactgct tcagcagttt cttctcaccc ggcgccatga cattaggatt gtaatcattg
     2221 gccaccacct catctgcttt tacccacagc acacagtcca caggctcctg tgcaaaagga
     2281 ctgacctcat gaagcaccct gcgcaggtga ttaagggcct gtatggcctc ttcttctggt
     2341 cgttcctggc tgaagtactg acgaatcatg ttgccaagta tctccggtgt cacagaatcc
     2401 cccattcctt cctcctttcc ttcatacgct gcaggtaacg ttcatagtgc cggggtttgt
     2461 tagggctaaa ggagagggtc cggcaccaga aatcattctt tatgagagtc ttacagatac
     2521 gtcgccagga cggaatatcc cggctcccca ggtcattctc ctgttcatcc ggaatgtcat
     2581 ccgggaagcc ccgcgtctga taccaacgca ggtagacagc aattttatta cggtaatgtt
     2641 ctttccggca tcacatccag caggaacatc gcatagctgc gccaggtatg atgaggcggc
     2701 ttcgtgatac gcttacacag ggcaaaatag gcaccactct cattggcata aagcgccccg
     2761 ctggcagccc ctgacacccg ttcacacatc ctggcccagg tttccggctc cagaacatgg
     2821 taaagccaca gtcccttacg ctgttcagga ccaaaaggtt cacagacacg catgttgcgt
     2881 aacggcacgc cggcacggta catcaggtca tacagaggat tgtaaatggc acaggttctg
     2941 gcgttatata tccagatatc gcgggccttc cagtcataca acggatacat ggtgtaataa
     3001 aacccttcag gcgatgccgt ggtccagggc ttatcatcgg catatctcag tttgcgctga
     3061 gataccagtc ccataaagcg attgagcgat tcatcagcac ggacaccagt cagtattgcc
     3121 actccacacc ggttaccggc aaaccaggaa gaaaatgccg gaacaaattc ttcaaacgtc
     3181 atggcatacc ggtaaaaggg aaaatacgtc atgtctgtaa tggcctcttc cggtggctgg
     3241 cgaacccagg tcactcctgg ctcccagcat atccactccg gctgaaactg cgagacgccg
     3301 tttaccgtgg tcagggaagt gccacccagt aaaaggtttc cgtcacatca tggtacattt
     3361 cccgcatctt ctgaatatgt tcaatagtgc accgatactg ggcctcccag tcaatgaaca
     3421 gaacagagaa atgacgtttt ctcctgcggg ccacctctgc cacaagatgg aaaagcacag
     3481 tggaatcctt tccacctgaa aaagacaggc ataccgacgg aaatgtttca aaaatccact
     3541 caatgcgggc gattgcggca cgcaaaacat tttctccggt aaggattttt tcggttaaaa
     3601 gcgtatcact cattttcctg gctttatgtc gaataattct gtttatgcat tcagacaaac
     3661 ttcattaata actcaccgct ttggggataa ataataacaa taaaactctt cagatattaa
     3721 tctggttgtt ggatttgtta tcacatcctt tatccacctg cttcttactg gtaacacatc
     3781 tggaaaatcc atgcattttc aacatatacc atcctttcaa cttgtaaata aggaaatatg
     3841 atgcagatgg tgaacaattc aaatatcatt ttgcaggaag tattcactgt aatagaatta
     3901 ccccctgata aagtttattc actaaggaat tattcaacag aaccattccc tgttagcttt
     3961 gagcaatagc acttattttt atatgtatca ggaatacgta cgttgatgta accaaaacaa
     4021 agaagcctgt gtccatgggg ttcgtacaat tgtgtgtggc acatgaccaa atcaggtttg
     4081 agacctcccg ctgcaatatt ttttattcag actttaacct gtcaggcctg acagaaacgc
     4141 tcccaacttc ccttctatat cactgtctat agtacgtaat cttagcaatg gcaggccgca
     4201 tttcttcagt atactgtttt taagttcatc acgttcagcc tgaacactgg tcagatgata
     4261 acctccatcc acttcaataa cccctaatgg agtcttcccg accttatagt aaacaacaaa
     4321 gtcacaactg gcccggttac gcatgaataa ctgttcacgg tgtgtaaaat ccccccgctc
     4381 cagcaacacc aactgattca gcgctatttg tgaatgaaac atcatactcc gataactatc
     4441 ctgcgacagg atatcccgca acaggcaggc cacaatctgt tctgacttaa agtgtgaatc
     4501 atttgaattc aatctactat tcagtcgttc cagtgatttg tcatactcac tgtacagcaa
     4561 atcaaatgct gatatgaccg ggctctcgaa aatctctccg tcatcagcgt agtatttgat
     4621 gtaacgaatc agcgccgcaa tatgtccagc gtggcgttca aatacatcat taccagtcac
     4681 cagcgtaaat ttatttcggg cccttgaaac agcgacattc accagttcag gattatcaac
     4741 aaacgcgatg ttccgactgt gctggctgct gcgcttctta tccagcacag tggaaaacac
     4801 aatttcatcg cactcccgtc cctgaaattt atgcaccgtc gatttcacaa aatcagcagg
     4861 cagcactgtt tcagccagat taacctgtgc attatatggg gcgatatatc ccctgctact
     4921 ttcctcatcc cagtaatgtc cttccagaga ttcaagctcc ctgagatttg aaaaattacg
     4981 ggtatggttt ccttttgccg tgatgatcag agataatgag gcttcacctg aatcaagcgt
     5041 tagcggtatc agagcattat cataaaactg cttattacaa aattgaatga ttttaggatg
     5101 acagcgataa tgctctttaa gaagagttac tggcaccata ttcctgaaca acatacaaac
     5161 cgaatcaagt agactatatt tctcacaatt atagtattct gccggtggag atgggctgtt
     5221 cgggagtaat acaggaacat gtggaagttg ctttcgatcc cctacaacga ttacattacg
     5281 ggcacatccc agtcccagaa tgcccggaac aatatcctgc tgtgaggctt catcaataat
     5341 cacataatca agaagagccc ccttaccgat agagttaata atagaatgcg tgctgctacc
     5401 aataaccgga aaacgcttta taaaacggtt aaattcatcc ctgtatgtct ctgccgagaa
     5461 tgaatttctt gttgaaatat ttcttcgaag atgttgtttt aaataaagca cagaccatga
     5521 tgtcaggcgc tccagtaaaa ttttaaaatc agattccttt aatacttcct catattcatt
     5581 cagagaaact tgtttttccc ggatgaattt ttcataataa gaaagctgaa gagaagtaaa
     5641 tagtgccagc cttctctccg gaatcaccaa tggtttgact ttcagtatcc tgaagtttaa
     5701 caacaaatca attctgtttc tgaatccaat ccgcctgtca gaaaggtaat gaatataagc
     5761 catcaggtca gttgtttttc gtgaggaaag tcggtatttg tgagaaaaaa gctcctccgg
     5821 tcgaagattc tgactctgcc attgctgcag gtatttatac tcaatattca gctcattaat
     5881 ttctgctttt aacgaagcga cctggttaat ggcattcagg tgttttttca cttgttgaag
     5941 tacatcatca atcgcatttg cagacggtga gtcaggtaag acttcctcag aacgcgaaat
     6001 cgacgttgaa aaaaactgtt gcctgttctc cgtactcccc agtcgtgcca ccacatatcc
     6061 cagttgctgt ttgtccatct tttcataaac attactgaca gctgagttat tattggac
//
LOCUS       DAZGLJ010000255         6099 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000255 DAZGLJ010000000
VERSION     DAZGLJ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.255"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            64..840
                     /gene="exuR"
                     /locus_tag="ACHK49_004354"
     CDS             64..840
                     /gene="exuR"
                     /locus_tag="ACHK49_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO9264874.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1080..1163
                     /gene="yqiM"
                     /locus_tag="ACHK49_004355"
     CDS             1080..1163
                     /gene="yqiM"
                     /locus_tag="ACHK49_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO9264875.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1185..1847
                     /gene="yqjA"
                     /locus_tag="ACHK49_004356"
     CDS             1185..1847
                     /gene="yqjA"
                     /locus_tag="ACHK49_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO9264876.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1851..2234
                     /gene="mzrA"
                     /locus_tag="ACHK49_004357"
     CDS             1851..2234
                     /gene="mzrA"
                     /locus_tag="ACHK49_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO9264877.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2381..2749
                     /gene="yqjC"
                     /locus_tag="ACHK49_004358"
     CDS             2381..2749
                     /gene="yqjC"
                     /locus_tag="ACHK49_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO9264878.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2787..3092
                     /gene="yqjD"
                     /locus_tag="ACHK49_004359"
     CDS             2787..3092
                     /gene="yqjD"
                     /locus_tag="ACHK49_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO9264879.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3095..3499
                     /gene="yqjE"
                     /locus_tag="ACHK49_004360"
     CDS             3095..3499
                     /gene="yqjE"
                     /locus_tag="ACHK49_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO9264880.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAIIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3489..3788
                     /gene="yqjK"
                     /locus_tag="ACHK49_004361"
     CDS             3489..3788
                     /gene="yqjK"
                     /locus_tag="ACHK49_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO9264881.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3974..4366
                     /gene="yqjF"
                     /locus_tag="ACHK49_004362"
     CDS             3974..4366
                     /gene="yqjF"
                     /locus_tag="ACHK49_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO9264882.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4436..5422
                     /gene="yqjG"
                     /locus_tag="ACHK49_004363"
     CDS             4436..5422
                     /gene="yqjG"
                     /locus_tag="ACHK49_004363"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO9264883.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5715..6080
                     /gene="yhaH"
                     /locus_tag="ACHK49_004364"
     CDS             5715..6080
                     /gene="yhaH"
                     /locus_tag="ACHK49_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO9264884.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1526 a         1510 c         1541 g         1522 t
ORIGIN      
        1 atggcatacc catgaataaa gtggtatgat aacttcgtcc gcattccact ccgggagacg
       61 catatggaaa tcactgaacc acgccgtttg tatcaacaac ttgccgctga cctgaaagag
      121 cgcatcgaac agggcgtata tctggtaggt gataaactgc ctgcagaacg ctttattgcc
      181 gatgaaaaga acgtcagccg tacggtagtt cgtgaagcca tcatcatgct ggaagttgaa
      241 ggctatgtcg aagtgcgtaa aggttcaggt attcatgtgg tttccaacca gccgcgccat
      301 cagcaggcgg ctgacaataa tatggaattc gccaattacg gtccgtttga gttgcttcag
      361 gctcgccagc tcatcgaaag taatattgcc gagttcgcgg caactcaggt aacgaaacaa
      421 gacatcatga aactgatggc cattcaggaa caggcgcgcg gcgaacaatg cttccgtgat
      481 tccgagtggg atttacagtt ccacattcag gtagccctgg cgacgcagaa ctccgccctg
      541 gcggccatcg ttgaaaaaat gtggacccaa cgtagccata acccgtactg gaaaaaactg
      601 cacgaacaca ttgattcccg taccgtcgat aactggtgtg atgaccacga tcaaatcctc
      661 aaggcgctga ttcgcaaaga tcctcatgct gctaagctgg caatgtggca gcacctggaa
      721 aacaccaaga tcatgttatt caacgaaacc agcgacgact tcgagttcaa tgccgaccgc
      781 tatctgttcg ccgaaaaccc ggtagtgcat ctcgatacgg ccaccagcgg cagtaaatga
      841 aatacctgtc cgacaggcgc tgcgacgcgc ctgtttgtct cgtcggtaag cgaaaagtat
      901 aaagtgtcag cctgtgtaaa tcctctcgcc accctcccct gcattcagca aaatcagact
      961 ccacggacat gtaattttga taacgaacaa cgttgacctt tgttacaatt agattcaatt
     1021 tgaatttatg tttttgaatg ctttcttatc tcacgattta acagggaata gttcaggctg
     1081 tgttgatgta tcaaacccgc agaacatacc aaaacagcaa taacattgcg gtagtgcatc
     1141 ttttaaaacc agcgtggcgt taaccgattc accaggaata atgaatggaa cttttgaccc
     1201 aattgctgca agccctgtgg gcgcaggatt ttgaaaccct ggccaatcca tcgatgattg
     1261 gcatgttgta ttttgtcttg tttgtaattt tgttccttga aaacggcttg cttccagcgg
     1321 cctttttacc gggcgacagt ttactggtat tggtcggcgt gttgattgcg aaaggcgcga
     1381 tgggttaccc gcaaacgatt ctgctgctga ccgttgccgc cagcctcggc tgctgggtca
     1441 gctatattca ggggcgatgg ctgggcaata cccgcaccgt acaaaactgg ctatctcatt
     1501 tacccgcgca ttatcatcaa cgcgcacacc atctttttca taaacacggt ttatcggcgc
     1561 tgttaattgg tcgctttatt gcgtttgtca gaacactgct gccgacgatt gccgggttat
     1621 cagggctgaa taacgcgcgc tttcagtttt tcaactggat gagcggtctg ctgtgggtat
     1681 tgatcctgac aactctgggt tacatgctcg gcaaaacgcc ggtattttta aagtacgagg
     1741 accagctgat gtcatgcctg atgctgctcc cggtggtgct gctggtgttt ggcctggcag
     1801 gttctctggt cgtgttatgg aaaaagaaat atggaaatcg ggggtaaggg atgcaaatac
     1861 ctcgcatgtc gcttcgccag ctagcctggt ccggcactgt tttacttctg gtcggcacgc
     1921 tgttgctggc ctggtccgcg gttcgccagc aagagtctac gctggcgatt cgtgccgttc
     1981 atcaaggcac aacgatgcca gacggttttt cgatctggca tcaccttgac gctcatggca
     2041 ttcctttcaa aagtatcacc cccaaaaacg acactctgtt aattacattt gactccagcg
     2101 accagagcgc cgccgcaaaa gcggtcctcg acagaacatt gccccatggc tacatcattg
     2161 cgcagcagga caataacagt caggctatgc agtggctgac ccggttacgg gataactctc
     2221 atcgcttcgg ataacttcct gttatccgaa acatatcact cactttggtg atttcaccgt
     2281 aactgtctat gattaatgag gcggtggtta acactaccgc ttcgttgtct ggacaatcgt
     2341 tcctttgtaa taggtccgaa tcacaatgga aggttcaaga atgaaatacc gcatcgcttt
     2401 agctgtttct ctctttgctc ttagtgccgg tagttatgcc actaccctgt gtcaggaaaa
     2461 ggagcaaaat atccttaagg agatcagcta tgccgaaaaa caccaaaacc agaatcgtat
     2521 tgacggtctg aataaagccc tgagtgaagt ccgggccaac tgttcagata gccagctgcg
     2581 tgccgatcat cacaagaaaa tcgcaaagca gaaagatgag gtggcggaac gccagcaaga
     2641 tttagccgag gcgaagcaaa aaggcgatgc cgataagatt gccaaacgcg aacggaaact
     2701 ggcagaagcg caggaagagc tgaaaaagct ggaagcgcgc gactactaac tcacaatagt
     2761 cactacttac tcacctggag aaaactatgt cgaaagaaca cactacggaa catctgcgtg
     2821 ctgagttgaa atccctttcc gatacgctgg aagaggtgct tagctcatct ggcgagaagt
     2881 cgaaagaaga gttgagtaag attcgtagca aagcggagca ggcactgaaa cagagccgtt
     2941 atcgcctggg tgaaaccggt gatgccatcg ccaaacaaac ccgtgtcgcg gcggcgcgtg
     3001 ctgatgagta tgtgcgtgaa aatccgtgga ccggcgtggg cattggcgct gcaatcggtg
     3061 tagtgctcgg cgttctgctg tcgcgtcgtt aattatggcg gacactcatc acgcgcaagg
     3121 gcccggtaaa agcgttctgg gcatcgggca gcgaattgtt tccatcatgg ttgaaatggt
     3181 ggaaacacgt ctgcggctgg cggtggtgga gctggaagag gaaaaagcta atctctttca
     3241 acttttactg atgctgggcc tgacgatgct tttcgctgca tttggtctta tgagcctgat
     3301 ggtgctaatt atttgggcgg ttgatccgca atatcgcctg aatgcgataa ttgccaccac
     3361 cgtggtgttg ctgctactgg cactaattgg cggtatctgg acgttacgta aatcgcgtaa
     3421 atccaccttg ctgcgccata cacgccatga gctggcaaac gatcggcaac tgctcgagga
     3481 ggagtcccgt gagcagtaaa gtcgaacgtg aacgacgtaa ggcgcaactg cttagccaga
     3541 tccagcaaca acggctggat ctttccgcca gtcgtcgtga atggctggag gcaacaggcg
     3601 cttacgatcg tcgctggaat atgctgctaa gtctgcgctc ctgggcgctg gttggcagta
     3661 gcgtgatggc gatctggacg attcgccatc ctaatatgct ggtccgctgg gccagacgcg
     3721 gttttggcgt atggagcgcc tggcgtctgg ttaaaacgac cctcaagcag caacagcttc
     3781 gcggttaact tccctctggc cggagcaatt ccggccttat ccctcaaatt tcttgaagat
     3841 ttttgacagt tttccttgct aacaatcatc attcaccacg tttatgattc tctccatcga
     3901 cagcaacgac gctaataccg cgcccttgca caaaaaaaca atcagcagcc tgagtggcgc
     3961 agtggagagt atgatgaaaa aattagaaga tgttggtgta ctggtagcgc gcattttaat
     4021 gccgattctg tttattaccg caggctgggg aaaaatcacc gcttactctg gtacccaaca
     4081 atatatggaa gcaatgggtg tcccgggttt tatgctgccg ctggtgattc tgcttgagtt
     4141 cggtggtggt ctggcaatcc tgttcggttt cctgactcgc accacagccc tgtttactgc
     4201 gggctttacg ctgctgacgg catttttatt tcacagcaac tttgctgaag gcgtcaactc
     4261 gctgatgttc atgaaaaacc tgacaatttc tggcggattc ctgctgctgg caattaccgg
     4321 tccgggcgcg tatagcatcg accgcctgct gaataaaaag tggtaagcac gcgctatact
     4381 taacgataaa aaacgaggag gaagctcctc gtttttgcta ttggaggaga gaaaaatggg
     4441 gcaactgatt gacggcgtct ggcatgacac ctggtacgat accaaatcta ccggcggtaa
     4501 atttcaacgt tcagcttccg catttcgtaa ctggctcact gccgatggcg cacctggccc
     4561 cactggcaca ggcggtttta tcgcagagaa agatcgttat catctctatg tttcactcgc
     4621 ctgcccgtgg gcgcaccgca cgctgatcat gcgcaaactc aaaggactgg aaccgtttat
     4681 ttccgtttcc gtagtgaacc cgctgatgct ggaaaacggc tggacctttg atgacagttt
     4741 tccgggagca accggagata cgctctatca acatgaattt ctgtatcagc tttatctcca
     4801 cgccgatcca cactacagcg gacgagttac tgttcccgtg ctgtgggaca aaaagaacca
     4861 caccatcgtc agcaacgaat cagcagaaat catccgcatg ttcaataccg cgtttgatgc
     4921 gctgggcgca aaagcgggtg attactaccc accagccctg caaacgaaaa ttgacgaact
     4981 taacggctgg atttatgaca ccgttaacaa cggcgtgtat aaagctggtt ttgccaccag
     5041 ccagcaagcc tacgacgagg cggtggcgaa agtgtttgaa tcgctggcgc gactggagca
     5101 gattttaggc cagcatcgtt acctgaccgg caaccagcta accgaagccg atattcgcct
     5161 gtggaccacg ctggtccgtt ttgatccagt gtatgtgacc cacttcaagt gtgataagca
     5221 ccgcatcagc gattacctta atctgtatgg cttcctgcgt gatatctacc agatgccggg
     5281 aattgccgaa acagtcaatt tcgatcacat ccgtaatcat tacttccgca gccataagac
     5341 catcaaccct acggggatta tttcaattgg tccatggcag gatctcgatg aaccgcatgg
     5401 acgagatgtt cgcttcggtt aaaaaaaggc accttacgag gtgcctctga taattagcta
     5461 agtgcatgtt gttcatgtcg gatgcggcgt gaacgcctta tcaaacctac aaaacattta
     5521 tattcaatat gttgcaatga ccgcgcaggc cagataagcg tagcgcatct ggcgattttg
     5581 cgcttgtcat cagtcttagc gcgccttcac agcactttat taattccttg aacgaatatt
     5641 tactgccatt tcattcacgt ctattcttaa tttgctgctt taaaaacaag tgattgagca
     5701 aaattgaggc aaaaatggac tggtatctga aagtactaaa aaattatgtc ggtttccggg
     5761 gtcgtgcgcg gcgcaaagag tactggatgt ttattctggt caacatcatc tttacgttcg
     5821 tgctggggct gctggataaa atgttaggct ggcaacgcgc tggtggcgaa ggcatcctga
     5881 cgacaatcta cggtattctg gtgtttttac cgtggtgggc agttcagttc cgccgcctgc
     5941 acgacaccga ccgctcggcg tggtgggcac tgttgttctt aatcccgttt atcggctggc
     6001 ttattatcat cgtctttaac tgccaggcgg gtacgccagg cgaaaaccgc tttggaccag
     6061 atccgaaact ggaaccttaa aacaaatctg ctcgccgga
//
LOCUS       DAZGLJ010000256         6097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000256 DAZGLJ010000000
VERSION     DAZGLJ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.256"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..1222)
                     /locus_tag="ACHK49_004365"
                     /pseudo
     CDS             complement(1..1222)
                     /locus_tag="ACHK49_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
     gene            1205..1903
                     /gene="tagH"
                     /locus_tag="ACHK49_004366"
     CDS             1205..1903
                     /gene="tagH"
                     /locus_tag="ACHK49_004366"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO9264885.1"
                     /translation="MSDPASNGGINTPFMDLPPIYASPGDRNDDVSAAEMAQRHLAVT
                     PLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLDLQQQRNSLSDKHLRPLEDNP
                     LRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVRHHEEANRAAIVESLRVLLDA
                     FSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEELASDRQQGFEMLFNEVYAQV
                     YDRVLREKQREPEA"
     gene            1900..2424
                     /gene="tssJ"
                     /locus_tag="ACHK49_004367"
     CDS             1900..2424
                     /gene="tssJ"
                     /locus_tag="ACHK49_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO9264886.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKKYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            2427..3757
                     /gene="tssK"
                     /locus_tag="ACHK49_004368"
                     /pseudo
     CDS             2427..3757
                     /gene="tssK"
                     /locus_tag="ACHK49_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            3762..4523
                     /gene="icmH"
                     /locus_tag="ACHK49_004369"
     CDS             3762..4523
                     /gene="icmH"
                     /locus_tag="ACHK49_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO9264887.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            4532..>6097
                     /locus_tag="ACHK49_004370"
     CDS             4532..>6097
                     /locus_tag="ACHK49_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9264888.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGHVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQ"
BASE COUNT         1288 a         1778 c         1736 g         1295 t
ORIGIN      
        1 ccggtgcgag ggtgcgcacg ctctccagcc tgcccgtggc actgtagccg tattcccgcg
       61 tctgtcgcgg gccgctgatg cgcaccaggt cgccgttgtc attccacccg tagtcacggt
      121 catacaccag gctgttcagg tgctggctct gtaactggcc tgcgggggtg tatgtgctgg
      181 tcagttcata tgcggcatta ctgcctgccc tgctgccgaa gctgcgcacc gtctcacggt
      241 gcagcctgtc ccgcgtatac tcgaccagcg gcgtcccgcc cagcttcatg cccgccaggt
      301 aaccgctgcc gtaggtcagc cactccaccg gcggcaggct gtccggcgtg acgcggtttg
      361 ccagcccctg ttcgttgtag gcgtgtttcg tctcatgctg ccacagcagt tcccccgtct
      421 ccgggttctc caccgtctgc cgttcgcccg tcaggcggcc cttatcgtcg tacccgtagt
      481 ggaccgacac gcggtggcct tcactggtat ggctgagggt ggttaaccac ccgtgctcat
      541 catactgcca ctgctctgcc gggtcgccgt tcaccgtccg gtgcgtgatg cggtccgacg
      601 catcgtagtg ccagagggtg ataagcccct cgtcttcact ctgtgtgagt tttctggtca
      661 ggtcatagtg gtaacgttgc gtccggccgt caaaaccgcg ctgttcagtc agcctgtcaa
      721 ccgggtcata ccggaacgtg gactggctgc cgttctcgtt ggtcagcacg gtgatgcgcc
      781 cggcagcgtc ataccccatg ctgcgcgtca gaccgccttc cgtcacactc accggacgcc
      841 cgcgtttatc atatgcgatg gtgctgcgtt taccgtccgg tgataccgtt gctgtcaggt
      901 ctcctgcggc gctgtactca taacgggttt cacgcccctg cgcatccttc tgactgacca
      961 gttgcccacg cgggttataa ctgctgtaag tgctgatgcc ttcctcccgg tgaacggcga
     1021 tttgttgacc gtaccggtcg tactcatacc gcgtcgtgta ccccgagcag tccgtaaagg
     1081 ccagcagttg accgtaacgg ctccatgcca tctgttttgt actgcccgtc gcgtcctgta
     1141 tccctgtcgg cagctcactc gccggccgct ttcaggcatt gcggctgtcc ggccatcggc
     1201 accgctgtcc gatccggcaa gtaatggggg gatcaatact ccgtttatgg atctgccgcc
     1261 catttatgcc agccccggcg atcgtaatga cgacgtctct gcggcagaaa tggcgcaacg
     1321 ccaccttgcg gtcaccccct tactgcgcgg tcttggcggc tcgcttacca tgagcaattc
     1381 cgacgatgcg gatgattttc tggaggaggc cggacgaacg ttacaggccg caataaaagg
     1441 tctgctcgat ttgcagcagc agcgtaacag cctctcagac aaacatttgc gcccgctgga
     1501 agataacccg ctgcgcctga acatggatta cgccaccgcg ctcgacgtaa tgtttgccga
     1561 aggtaaaagc ccggtacatc tggcggctcc cgccgccgtc agtgaaagcc tgcgcaatgt
     1621 ccgccaccac gaagaagcta accgggcagc gattgtggag tcgcttcgtg tcctgctgga
     1681 tgctttctca ccacaaaatc tgctgcgccg ctttgtgcag taccgccgca gccatgaact
     1741 gcgccagccg ctggatgatg ccggagcatg gcaaatgtac agccattatt acgaagaact
     1801 ggcctccgat cgccagcagg ggtttgagat gctgtttaac gaggtctacg cccaagtcta
     1861 tgaccgggtg cttcgtgaaa aacagcggga gccggaagca tgaacaggaa ggcgtttctg
     1921 gcctgcgtgc tgatgtgcgt catattaacc ggctgcgaaa cggcgaaaaa aatcagtgag
     1981 gtgatcaaaa acccggatat ccaggtcggg agcctgaagg agcaaccttc ggaaataacc
     2041 gtcaccctgc tgaccgaacc ggacaccaat accaatgcgg aaggtgaatc agccgccgtg
     2101 gatgtgcaac tggtgtacct gactgacgac tcaaaattgc tggcagccga ctatgaccag
     2161 atagccagta cgccgctacc ggatgtgctg ggtaaaaaat atatcgacca ccaggatttc
     2221 aacctgttgc cggacaccat caaaaccctg ccgccggtaa agctggatga gaaaacgcag
     2281 tttattggtg tcgtggccta tttctccgat gaccaggcca ctgaatggaa acaaatcgag
     2341 acggtggaag gaaccggcca ccattaccgc ctgctggtgc atgtacgtca aagcagcatc
     2401 gaaatgaaaa aagaggatga ataattatgg caaccacccg caacaaggtg atgtggcagg
     2461 aagggatgct gatgcgtcca caccatttcc agcagcagca gcgttacaac gactacctgg
     2521 ataaccagcg tttccgggcc atgaatgatt tatcctgggg atttaccgaa ctcactctca
     2581 acaatgaact gctggcacag ggtaagatca tgattgacag cgcgtcaggc acactgcccg
     2641 acggcaccgt cttttctatc cccgaccagg acgcactgcc cgatccgctg cacccgcaac
     2701 attttccgga cgagagaagc cgcaatatct acctcgctct gccggtcgcc agtgatgtga
     2761 gaaatgaaat cagcgacggg cggcgaatcg ggcgttaccg gctgaattat gccgatgtcc
     2821 gggatttgca ttcagaagaa ggtgacacgc gaacactgac gctgggacaa ctgacgccgc
     2881 gcatatgagc ggtgcagaag atatgagcgc ctatattacg ctcccgcttt gccgtatcag
     2941 cgatcgccat gccgatggct ccctgacgtt ggatgacgat tttatcccct cctgccagaa
     3001 tattcaggtc agtaagaaac tgcgtgttta tctcaaagag gtacaggggg ccattggcgg
     3061 acgggcaagc gatctggcaa accgtattgg ctctccggcg cagagcggca tcgcggatgt
     3121 ggcggaattt atgatgttgc agttacttaa ccgtaaccag acccggttta cccatcgcgc
     3181 ccgccgatcc cagctccacc cggaagattt ctaccttgat cttgctgggt tgctgggtga
     3241 actgatgacc tttaccgagc cgtcgcgcct gccctgcccg cttgatgtgt atgatcatca
     3301 tgatctgacc aaaacattta aaacactgtt accggaagtc aaacgggcgc tgcataccgt
     3361 actgtcgcca agagcggtca atctgccgct gcatctgcgt gacggtatct ggcaggccga
     3421 tatccatgac acagaactgc tgcaatctgc cacctttgtg ctggccgtgg cggcaaacgt
     3481 gccggtcgat cagatccagc gtcagtttat ccagcagtcg aaaatttcct cgccggaaaa
     3541 aatccgcaat atggtcagtg tgcagatccc cggcattcca ttgcgcgcac tgatggtggc
     3601 tccccgccag cttccttacc attccgggtt cagctatttc gaactcgaca agagcggaca
     3661 ggcctggaca gaaatggctg ccgccggggc cgttgcactg catgtatccg gcagtttccc
     3721 ggatctgaac atgcaactgt gggcgataag agggtaagac gatggatgag gggtcactgt
     3781 ctctgccacc atttaccgga tacgacgaaa aaagccagcg cgactatcat ctcgcgcttc
     3841 gtggcaacag cctgaatccg atgatcgacg cagccacgcc gctcctcggt atggtgatgc
     3901 gcctttcaac catgaacagc cagacgatgc cggagcatct ttttgctcag gtggtgacgg
     3961 atgttcaggc cgtggagcaa ctgcttcagg agcagggcta cgaaccgggc gtgattatct
     4021 cgtttcgcta catcctctgc acgtttattg atgaagcggc gctcggtaac ggctggtcga
     4081 acaagaacga gtggatcaag cagtcattgc tggtccattt ccacaacgaa gcctggggtg
     4141 gagagaaagt tttcatcctg ctggagcgcc tgatccgtga acccaaacgt taccaggatc
     4201 tgctggaatt tctctggctc tgtttttctc tgggatttcg cgggcgctac aaagtggcag
     4261 cacaggatca gggggaattt gagcagatct accgccgcct gtaccacgtc ctgcataaac
     4321 tacgcggcga tgcccctttc ccgctgttac accaggacaa aaaaacccag ggcgggcgtt
     4381 atcagctcat cagtcgcctg accattaaac atattttttg tggtggcgtt gtcgtgctgg
     4441 cgttgtttta cctgttctat ctgttgcggc tggacagcca gacgcaggac atcctgcacc
     4501 agctaaacaa attacttcga taaggacgtt tatgatccag attgatcttc ccacgctggt
     4561 aaaacggctt aacctgttct cccgccaggc gctggagatg gccgcctctg aatgtatgag
     4621 ccagcaggca gcggaaatta ccgtcagcca tgtgcttatt cagatgctcg ccatgccacg
     4681 cagtgacctg cgggttatta cccgccaggg cgatattggc atggaagagt tgcgccaggc
     4741 gctgacggtg gagaactaca caactgcccg ttctgcggac agctacccgg cattttcccc
     4801 gatgctggtt gagtggctga aagagggctg gctgctggcg tcggctgaga tgcagcacag
     4861 cgaattgcgt ggcggcgtgt tgctgctggc cctgctgcat tcgccgctgc gttatatacc
     4921 gcctgctgcc gcccggctgt tgaccggcat taaccgtgac cgtctgcaac aggactttgt
     4981 gcaatggaca caggagtcgg cggaatcagt cgtgccggat gcagacggta aaggcgcagg
     5041 cacactgacg gacgctgctg acaccctgct tgcccgctac gccaaaaaca tgaccgcaga
     5101 cgcccgtaac ggcaggcttg acccggtact gtgccgcgac cacgaaatcg acctgatgat
     5161 cgacattctc tgccgccgcc gcaagaacaa cccggtggtg gtgggcgaag caggcgtggg
     5221 taaaagcgcc ttaatcgaag ggctggcgct gcgcatcgtg gcaggccatg tgccggacaa
     5281 gctgaaaaac accgatatca tgacccttga cttgggcgca ttgcaggccg gagcgtcggt
     5341 gaagggtgaa ttcgaaaaac gtttcaaagg gctgatggca gaggtcattt cctccccggt
     5401 gccggtcatt ctgtttatcg acgaagcaca taccctgatt ggcgcgggca accagcaggg
     5461 cgggctggat atctccaacc tgctcaaacc ggcgctggcg cgcggcgagc tgaaaaccat
     5521 cgccgccacc acctggagcg agtacaaaaa atacttcgaa aaagatgccg ccctgtcgcg
     5581 ccgcttccag ttggtgaagg tcagcgaacc caacgctgcc gaagccacca ttattctgcg
     5641 cggtctgtcg gcggtctatg aacagtctca cggcgtgctg attgatgatg acgccttgca
     5701 ggccgctgcg acattaagcg agcgttatct ctccgggcgt cagttaccgg acaaagcgat
     5761 tgatgtgctg gataccgcct gcgcccgtgt ggccatcaac ctgtcgtcgc cgccgaagca
     5821 aatctcggcg ctgaccactc tgagccacca gcaggaggcg gaaattcgcc agcttgagcg
     5881 cgagcttcgc atcggactgc gtaccgacac atcacggatg accgaggtgc tggtgcagta
     5941 tgatgaaacg ctgacggcgc tggatgaact ggaagcggcc tggcaccagc agcagacgct
     6001 ggtccgggag attattgcgc tgcgccagca gttactgggc gtggcagagg acgatgcggc
     6061 gccgttgccg gacgcagata ccgtggagga tacgcag
//
LOCUS       DAZGLJ010000257         6025 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000257 DAZGLJ010000000
VERSION     DAZGLJ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.257"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(144..551)
                     /locus_tag="ACHK49_004371"
     CDS             complement(144..551)
                     /locus_tag="ACHK49_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9264889.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            629..856
                     /locus_tag="ACHK49_004372"
     CDS             629..856
                     /locus_tag="ACHK49_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO9264890.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELLYDPKVYDEYRKTKRAGRLNNENHS"
     gene            840..1361
                     /locus_tag="ACHK49_004373"
     CDS             840..1361
                     /locus_tag="ACHK49_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO9264891.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            1342..2307
                     /locus_tag="ACHK49_004374"
     CDS             1342..2307
                     /locus_tag="ACHK49_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264892.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKLSESIGLKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDQQTAEQDFLTRNPDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVNLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDQFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            2348..2767
                     /locus_tag="ACHK49_004375"
     CDS             2348..2767
                     /locus_tag="ACHK49_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO9264893.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYSSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKARRSRLLLTPSCNVPFEPF"
     gene            2822..3919
                     /locus_tag="ACHK49_004376"
                     /pseudo
     CDS             2822..3919
                     /locus_tag="ACHK49_004376"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1202-like element ISEsa1 family transposase"
     gene            3992..4141
                     /gene="ydfW"
                     /locus_tag="ACHK49_004377"
     CDS             3992..4141
                     /gene="ydfW"
                     /locus_tag="ACHK49_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfW"
                     /protein_id="HFO9264894.1"
                     /translation="MDNARIDLRSKYYVKSKADHPWLTRRTQSHQQVKPPKLPKKKPD
                     PDKID"
     gene            complement(4578..4910)
                     /gene="flxA"
                     /locus_tag="ACHK49_004378"
     CDS             complement(4578..4910)
                     /gene="flxA"
                     /locus_tag="ACHK49_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFO9264895.1"
                     /translation="MSVTIQGNTSTVISNNSAPEGTSEIAKITRQIQVLTEKLGKISS
                     EEGMTTQQKKEMAALVQKQIESLRAQLEQLLRQQAEKKNKDATVQPDKKEEKKDDTNT
                     AGTIDIYV"
     gene            complement(5113..5418)
                     /gene="ydfV"
                     /locus_tag="ACHK49_004379"
     CDS             complement(5113..5418)
                     /gene="ydfV"
                     /locus_tag="ACHK49_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFO9264896.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLVFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMSVLLSRRRVSGDLTCR"
     gene            5443..5682
                     /gene="relB"
                     /locus_tag="ACHK49_004380"
     CDS             5443..5682
                     /gene="relB"
                     /locus_tag="ACHK49_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFO9264897.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            5682..5969
                     /gene="relE"
                     /locus_tag="ACHK49_004381"
     CDS             5682..5969
                     /gene="relE"
                     /locus_tag="ACHK49_004381"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFO9264898.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT         1645 a         1260 c         1528 g         1592 t
ORIGIN      
        1 aacaccgtgc ggtgtcttga tggaggtaat ttagttttct cattattttt cgtcaaggtt
       61 ttttgatgag aaaactcaag cgttggcgca agataaagcc aatacattga aatgtaaggc
      121 tttaaaattt tgtgaaggga tgattattga tgcttgttgc gtttgcgagc ctctaatagc
      181 tcggtgaaca ggcgattaaa attctcaacg cgggcgcgga gttcgctgag ttgtgcttgt
      241 tgctctgatt taggaagtgc gcgatacaaa cgcagcatct ctaactcatc ttccgataag
      301 tctaaggcac tatcgagtga aacaggggga gctggtgttt tgtcctcgtc gccaaacagt
      361 atccaagttg gtgaacattg caatacctcg gcgaggcgat gcaaattttg cccgcgcggg
      421 gctgtatggt cactttccca taatgaaatt gatgagccag atacgccagc agctttgctt
      481 aaaccgtttt gacttaagcc tacctgctta cgtctttctc taatgcgttg acctaaagtt
      541 ttctcgttca tatttagata tcttaataac ccttgacttg agattccttg agtgattagc
      601 attgagaaaa ctcaatattg gaggtgcgat gtttaaatca gacgtaatta atttttacgg
      661 tacgaaagcc aaagtagcga aagctgctgg cgttgaccca tctgctgttt ctcaatggca
      721 agagctggtt cctgaaggtc gcgcgatgcg tctacaggag gcatctggcg gcgagcttct
      781 gtatgatccc aaggtttatg acgaatatcg taagacgaag cgggcggggc ggttgaacaa
      841 tgaaaatcac tcctgaacag gctcgtgagg ctctggatgc ctggatatgt cgaccaggaa
      901 tgacacagga gcaggcgacg atattaatca ctgaagcatt ctgggctttg aaagagcgcc
      961 cgaacatcga tgttcagcgt gtcacagatg aaggtggcgc ggttgatcag cgagcacttg
     1021 gcgttaatcg agtgaagata ttcgaacgct ggaaggctat cgacaccagg gataagcgtg
     1081 aaaagttcac ggcgctagtg cctgcaatta tggaggctat tcggattaat gatttcaggt
     1141 tgtatcgtga aattagtgac ggaaaaagca tcacgtacat gatcgccggg ttaaacaaag
     1201 aatatggcga tgtggtggag tccggactgc tttttgctga tcctgccgta gtggatcgtg
     1261 aaactgacga acttatagaa aaagcaattg ctttcaaact tgcgtatcga cagcaatacc
     1321 aacaaaaagc tggatggaat tatgagcctt ctttttgctg aacgcccact ggttataaac
     1381 acacagcttg cgatgaagat tggcttaaat gaagccattg tgttgcagca gttgcattac
     1441 tggttgagag ataccaattc cggcatggaa tgtgatggtg ttcgctggat ttacaacaca
     1501 acggaacaat ggctggaaca gttcccattc tggtcagagt caacgttaaa gcgcgcgttt
     1561 gcaagtctga aaacgctggg gcttttgcgt tgtgaaaagc tcaataaatc aaagcgcgat
     1621 atgaccaatt tctacacgat caactacggg agcgagcttt tagatggtgg caaattgagc
     1681 gaatccatcg gtttaaaatg cgccgctcca tcaggtcaaa atgacacgat ggaagaggtc
     1741 aaaatgaaac gctccattgg ttcaaaacga cccaatgtca tcgggtcaaa atggcctgat
     1801 gatcttacag agaatacaac agagattact acagagaata aaaacacttt tcgtccggaa
     1861 gcttcgcaac cggaccagca gaccgctgaa caggattttt taacccggaa ccccgacgcg
     1921 gttgtgttta gtgcgaaaaa acgccagtgg ggtagcaggg aggatttggc gtgtgcgcag
     1981 tggatttggg ggcggatcgt gaacctttac gaacaggctg ccagcgacga tggagagatc
     2041 atgcgaccaa aagagcctaa ctggacagcc tgggccaatg acgtgcgcac aatgcggatg
     2101 ctggatggca gaagccacag acaaatttgc gaaatgtttg gtcgggtaca gcgagatcaa
     2161 ttctgggtaa aaaacatcat gagcccgtca aaactccgcg aaaaatggga cgaactggtc
     2221 atccgcctgg gacgttcacc tgtacagcgt tgtgtgaatc atatttctga accggataca
     2281 gaaattccgc ctggtttcag gggataagtg ttgattttag ggtataaagt aatgttcagg
     2341 aggacttgtg gcaaaagttt ttacacaaga agagcgggaa aaaattaaag ggcaggtggt
     2401 ggaactcgta cgccagagtg ggcgcgagac gttacgacaa ctggaagcta aaactggggc
     2461 aacaagatat ctgatgagcg ttctggccag agagctggtt gccagtggcg atgtatacag
     2521 ctctggttac gggttattcc cgtctgaaca ggcgcgtaag gactggcaaa acgcccgcaa
     2581 aaaactctcg agggcaaagg tgaagaaacc tgctgtggtt gatccggacc ttatctggtc
     2641 attacctgac ggagaaatac gtcgctacga cagtcgtcta aacataatct gtcgcgagtg
     2701 ccggaaggca aggagaagca ggctattgtt aacaccaagc tgtaatgtcc cctttgaacc
     2761 attctaaaat gtccccagac aattctctgg gggatttttc atgatcaaag agactgttac
     2821 gatgagtcat aaggaactcc accgacttca gattattcag gagtcaatta atcgacatat
     2881 tactcaggaa caagctgcgg cacgcattgg catttctatt cggcaggtta aacgtctggt
     2941 gcaacggtat agaaatgaag ggccttctgg tctggtttcc caccgacgtg gaaagcgtcc
     3001 taataattcc ttttctactg aattcagagc aacagtaatt tcactcctca aaggccgtta
     3061 cgctgatttt ggacctacgt ttgcgtgcga aaaattgcgc gagatacacg gtttatcttt
     3121 atccgttgaa actctcagaa agtggatgat agaagagggg ttatggcgtg aacgccgtcg
     3181 taaaattgcc cgtatatatc aacgccgcat gcgacgacca tcttacggtg aactgatcca
     3241 gattgatggc tcacctcatg actggtttga aaatcgaggc cccagatgta cactgatcgt
     3301 tttcattgat gatgccacca gtgcgttgat ggcgttgcgt tttgtgcctg ctgaaacaac
     3361 ccgggcttac atggaaaccc tccggggtta ccttaatgat catggcgtac cgctcgctct
     3421 ctactctgat agacacagta tattcagggt aaataaccca gagcgggaag gtgagctgac
     3481 ccagttcact cgtgcgataa agacactggg catcgagcca atccatgcca acagcccgca
     3541 ggcaaaaggg cgggtagagc gcgccaatca gacactacag gacaggctgg tcaaagaaat
     3601 gcggcttcag aatatcagtg atattgaaac agcaaatgca tggttgccga cctttattga
     3661 agcctataac aaccggttcg ctacgtcgcc tcgtactact gataatgctc atcttgatgt
     3721 gcaccattct gaagaggaac tgggttatat cttcagccta caggcgaagc gcgttctgtc
     3781 taaaaatctc actttccagt acaaaagcag tgcgtttcag gtacgcagtg agggccgggg
     3841 atatcgactt aggcattcgg ttgttactgt atgtgagaac tttgacggtg aaattaacgt
     3901 tctgtatgac gggaaagcgc tgggctggga aaagtatgtt gatggcccgg agcctatacc
     3961 actggatgat gaaaagagtg tccatgaacg agtggataat gcccgtattg atttacgctc
     4021 aaaatactat gttaaatcta aagctgacca tccctggctt acgcgccgaa cgcaaagtca
     4081 tcagcaagtt aagcccccga agttacctaa aaagaagcct gatcccgata aaatagattg
     4141 aaaccaagat cgattcggtt gagtgcatat ccattcatag ggtagattct taagtcgcgt
     4201 ttctgatgtt cattttcggg tggtttgtta cttgttttac cggggatatg ccagaaacgc
     4261 gctgagtcag tctgggcggt gcgcgtaatg aggcgttatg gtaaatagcc tatgctaatg
     4321 tccgctaaga gcaagaagcg gaagttggca gttttgtgga ctgtccccac aaaagtgact
     4381 acagaaatag ttgcaattca taattgatca tgggttgtca gttaaactcg tggcgattta
     4441 aatagactaa ttgggagtgc gtccattact tatatcttgt aatgttaact atcagaaatg
     4501 atacaaagat aatatgtctt taaagaaaag gctgatggcg aaaagtggcc cgatgagggc
     4561 cacaatacgg ctgtcactta gacgtaaata tcaatggtgc cagcggtatt tgtatcgtct
     4621 tttttctctt cttttttatc aggctgaact gtcgcgtctt tattcttttt ctctgcctgc
     4681 tgccttaaca actgctccag ttgagccctg aggctttcaa tttgcttctg taccaatgca
     4741 gccatttctt ttttctgctg tgtcgtcatc ccctcttccg atgagatttt cccaagcttt
     4801 tcagtcagca cctgaatttg tcttgtgatt ttggctattt ctgatgttcc ttccggggcg
     4861 gagttgtttg aaataacggt tgaggtattt ccctgaattg tgacagacat agatttctcc
     4921 ttttaaaaaa gcactatcgg catgcacaaa aaaatcttta atcgtatttc ttgtgtcatt
     4981 aattgtttga tgttcagatt gttttcctcg cgggctggcg cgcctcagaa agtaaagctt
     5041 gttgacaggg gtaaacgttc ggcaataatt ttctgccgca tgcgggtgtt gcataaaacg
     5101 tgctacgttc ctttatcgac aggtcaggtc accgctcacc cgccgacgag aaagcaacac
     5161 tgacatgcta aagcaaaaaa tagatgaata agttgagttg tgcatatgta gcctgaccgt
     5221 cacaaagtat atggtgtctg taccagtaag atgatggccg gactctttaa aaacgagctg
     5281 acctgcacaa tacaggatgg acttagcaat ggctgctcct ggcacaaagc ggacagtgat
     5341 caccgttctt acgactactt tctgacttcc ttcgtgactt gccctaagca tgttgtagtg
     5401 cgatacttgt aatgacattt gtaattacaa gaggtgtaag acatgggtag cattaacctg
     5461 cgtattgacg atgaacttaa agcgcgttct tacgccgcgc ttgaaaaaat gggtgtaact
     5521 ccttctgaag cgcttcgtct catgctcgag tatatcgctg acaatgaacg cttgccgttc
     5581 aaacagacac tcctgagtga tgaagatgct gaacttgtgg agatagtgaa agaacggctt
     5641 cgtaatccta agccagtacg tgtgacgctg gatgaactct gatggcgtat tttctggatt
     5701 ttgacgagcg ggcactaaag gaatggcgaa agctgggctc gacggtacgt gaacagttga
     5761 aaaagaagct ggttgaagta cttgagtcac cccggattga agcaaacaag ctccgtggta
     5821 tgcctgattg ttacaagatt aagctccggt cttcaggcta tcgccttgta taccaggtta
     5881 tagacgagaa agttgtcgtt ttcgtgattt ctgttgggaa aagagaacgc tcggaagtat
     5941 atagcgaggc ggtcaaacgc attctctgaa ccaaagcatg acatctctgt ttcgcaccga
     6001 aggtgacact tctgctttgc gttga
//
LOCUS       DAZGLJ010000258         5990 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000258 DAZGLJ010000000
VERSION     DAZGLJ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.258"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(33..1679)
                     /gene="fumB"
                     /locus_tag="ACHK49_004382"
     CDS             complement(33..1679)
                     /gene="fumB"
                     /locus_tag="ACHK49_004382"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO9264899.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            1878..3053
                     /gene="manA"
                     /locus_tag="ACHK49_004383"
     CDS             1878..3053
                     /gene="manA"
                     /locus_tag="ACHK49_004383"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO9264900.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSILQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            3154..4662
                     /locus_tag="ACHK49_004384"
     CDS             3154..4662
                     /locus_tag="ACHK49_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO9264901.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(4707..5972)
                     /gene="uidC"
                     /locus_tag="ACHK49_004385"
     CDS             complement(4707..5972)
                     /gene="uidC"
                     /locus_tag="ACHK49_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO9264902.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
BASE COUNT         1564 a         1530 c         1404 g         1492 t
ORIGIN      
        1 tgtaaaaaaa ccgccccgaa gggcggctct gtttatttca cacagcgagt gcactgtgtg
       61 agttgtatct gctggaagaa gtcatttcct ttatcatcca caaggataaa cgccgggaaa
      121 tcttccactt caattttcca gatggcttcc attcccagtt ccggatattc aacacattcc
      181 aggctcttaa tacttccctg cgccaataca gcggccggac caccgatact gccaaggtag
      241 aagccgccgt gttttttaca ggcatccgtc acctgctggc tgcggttgcc tttcgccagc
      301 atgatcatac ttccgccctg cgcttgcagt tgatcgacat aagaatccat ccgtccggcg
      361 gtcgttgggc caagagaacc ggaggcataa ccttccggcg ttttggccgg acccgcgtag
      421 taaatcggat gatctttaat gtactgcggc agcccttcac cgttatccat ccgctctttc
      481 agtttggcgt gagcaatatc acgaccgacg ataatcgtgc cgttaagcga taagcgtgta
      541 gaaacgggat actgcgacaa ctgtgcgagg atctctttca tcggacggtt aaggtcaacg
      601 cgcaccgctt cgccttctcc cgctttacgc agctcttcag ggatatattt acctggatta
      661 tgttccagtt tttcgatcca gatcccctga cggttgatct tcgctttgat attacggtca
      721 gcagagcagg aaacacccat accaaccgga caggatgcgc cgtgacgtgg caggcgaatc
      781 acgcgtatgt cgtgagcgaa gtatttacca ccaaactgcg cacccagacc gagattttgc
      841 gcttcgatca gcaattcttt ttccagttcc acatcgcgga atgcctgacc gtgctcattc
      901 ccttccgttg gcagttcgtc atagtattta gcggaagcca gtttcactgt tttaaggtta
      961 gtttctgcag aagttccacc aattacgaac gcaatatgat acggaggaca ggccgccgta
     1021 cccagcgtgc gcatcttctc aaccaggtaa tttttcagtt tccccggcgt tagtaacgct
     1081 ttggtttcct gatagagata cgtcttgttt gccgaaccac cacctttggc gatacagagg
     1141 aatttgtact cgtcgccatc aacggcataa agatcgatct gcgctggcag attggtgccg
     1201 gtattcacct ctttatacat atccagcggc gcgttttgcg agtagcgcaa attatcttcg
     1261 atataagtgt tatagacacc gcgcgccagc gccgcttcat cacagccacc ggtccataca
     1321 cgctgccctt ttttaccaac aataatcgca gtgccggtat cctgacaggt tggcagaacg
     1381 cctttcgccg cgatgtcgga gttacgcagg aattgcagcg ccacatattt atcattttcg
     1441 ctggcctccg ggtcacgcag aatgtcggcc acctgttgtt ggtgagccgg acgcagcata
     1501 aacgacgcat catgaaacgc ctgacgcgcc aacagagtta acgcttcggg tgcgactttc
     1561 aaaatctcct gcccttcaaa ttcagatacg ctaacgtgtt cgctggttag caggtaatac
     1621 tcagtatcat cttttttgag tggaaaagga gcctgataat gaaagggttt gtttgacatt
     1681 gttctctcac ttactgcctg gtttggttat gctctgggcg ggtgttccgt tgccctgtta
     1741 aaagcgagta acaatatcct acacactttt ttaacaaaaa ctgagactag tacgactttt
     1801 tgcggctcca ggttacttcc cgtaggattc ttgctttaat agtgggatta atttccacat
     1861 taaaacaggg attgatcatg caaaaactca ttaactcagt gcaaaactat gcctggggca
     1921 gcaaaacggc gttgactgaa ctttatggta tggaaaatcc gtccagccag ccgatggccg
     1981 agctgtggat gggcgcacat ccgaaaagca gttcacgagt gcagaatgcc gccggagata
     2041 tcgtttcact gcgtgatgtg attgagagtg ataaatcgac tctgctcgga gaggccgttg
     2101 ccaaacgctt tggcgaactg cctttcctgt tcaaagtatt atgcgcagca cagccgctct
     2161 ccattcaggt tcatccaaac aaacgcaatt ctgaaatcgg ttttgccaaa gaaaatgccg
     2221 caggtatccc gatggatgcc gccgagcgta actataaaga tcctaaccac aagccggagc
     2281 tggtttttgc gctgacgcct ttccttgcga tgaacgcgtt tcgtgagttt tccgagattg
     2341 tctccatact ccagccagtc gcaggtgcac atccggcaat tgctcacttt ttacaacagc
     2401 ctaatgccga acgtttaagc gaactgttcg ccagcctgtt gaatatgcag ggtgaagaaa
     2461 aatcccgcgc gctggcgatt ttaaaatcgg cgctcgacag tcagcagggt gaaccgtggc
     2521 aaacgattcg tttaatttct gaattttacc cggaagacag cggcctgttc tccccgctgt
     2581 tactgaatgt ggtgaaattg aatcctggcg aagcgatgtt cctgttcgct gaaacaccgc
     2641 acgcttacct gcaaggcgtg gcgctggaag tgatggcgaa ctccgataac gtgctgcgtg
     2701 cgggtctgac gcctaagtac attgatattc cggaactggt tgccaatgtg aaattcgaag
     2761 ccaaaccggc taaccagttg ttgacccagc cggtgaaaca aggtgcagaa ctggacttcc
     2821 cgattccagt ggatgatttt gccttctcgc tgcatgacct tagtgataaa gaaaccacca
     2881 ttagccagca gagtgccgcc attttgttct gcgtcgaagg cgatgcaacg ttgtggaaag
     2941 gttctcagca gttacagctt aaaccgggtg aatcagcgtt tattgccgcc aacgaatcac
     3001 cggtgactgt caaaggccac ggccgtttag cgcgtgttta caacaagctg taagagctta
     3061 ctgaaaaaat taacatctct tgctaagctt attaaaggct tataacacct tcaggcggcc
     3121 agtccgcctg atttcatttt atggataatc attatgaata aatcgctggt agcggtaggc
     3181 gtcattgttg cgctaggcgt agtctggaca ggcggcgcat ggtatacagg caagaagatt
     3241 gaaacccatc tcgaagacat ggtcgcgcag gcgaacgcgc aactcaaact gacagctcct
     3301 gaatccaacc tggaagtgag ttatcaaaac tatcatcgcg gcgtattcag cagccagttg
     3361 caactgttgg tgaaacccat tgccgggaaa gaaaatccgt ggattaaaag cggtcagagc
     3421 gtcatcttca acgaatcggt tgatcatggt cccttcccgc ttgcccagct taaaaaactg
     3481 aacctgatcc cgtcgatggc atcaattcaa accacgctgg ttaataacga agtaagcaaa
     3541 ccactgtttg atatggcaaa aggtgaaacg ccttttgaga ttaactcgcg cattggttac
     3601 agcggtgatt ccagttccga tatttcgctc aagccactga attacgagca aaaggatgaa
     3661 aaagtcgcct ttagcggcgg cgagttccag ttaaatgctg acagagacgg caaagccatc
     3721 tccctttccg gggaggcgca aagtggtcgg atagacgcag ttaacgaata caaccagaaa
     3781 gtgcagttga cctttaataa tctgaaaacc gacggttcca gcacgctggc aagttttggt
     3841 gagcgcgtag gaaaccaaaa actgtcactg gaaaaaatga ccatttcagt ggaaggcaaa
     3901 gaactggcac tgctggaagg catggagatc agcggtaaat cggatctggt caatgacggt
     3961 aaaacgatca atagccaact ggattactcg ctaaacagcc tgaaggtaca gaatcaggat
     4021 ctgggcagcg gcaagctgac tttaaaagtc ggccagattg atggtgaagc ctggcatcag
     4081 tttagccagc aatataacgc gcaaactcag gcgctgctgg cacagccaga aattgccaac
     4141 aatcccgaac tttatcagga gaaagtgacg gaagccttct ttagcgccct gccgctgatg
     4201 ttgaaaggcg atccggtgat tactatcgcg ccgctaagct ggaaaaacag tcagggtgaa
     4261 agtgcgctga atctgtcgct gttcctgaaa gatccggcaa cgactaaaga agcgccgcaa
     4321 acgctggcgc aggaagtaga tcgttcggtt aaatctctgg atgcgaaact gaccattccg
     4381 gtggatatgg caactgagtt tatgactcag gtagcgaagc tggaaggtta tcaggaagat
     4441 caagcgaaaa aactggcgaa acagcaagtt gaaggtgcat cagcaatggg gcagatgttc
     4501 cgtctgacca ccttgcagga caataccatc accaccagcc tgcaatatac taacggtcag
     4561 ataacgttaa acgggcagaa aatgccactg gaagatttcg ttggtatgtt tgcaatgccg
     4621 gcattaaatg ttccggtcgt acccgctatt ccgcagcagt aattcacaga caaaacgccg
     4681 gagatttttc tctccggcgt tattttttac ttcagcataa agtcatactg aatactcatt
     4741 ttcacatccc agtaattcag gcgatcgccc agcgtactgc catcgcgctt ttcccgccct
     4801 ttatcggcag caagaaagaa attcatccct tgtaacggcc cttcgctgac ccgatagttc
     4861 ataccaccta agatacccgt gccttcaatc cgcacatctc gtttactgga ttcatagccg
     4921 cgagtcacaa tcggtgcaaa tgtcagcgta aattgcggcg ttaaacgata gttgacgccc
     4981 aattgccagg actgcattcc ttcacggtta cgatcaagac ttaatgaaaa gggatagcca
     5041 atgtcggtat ccaccacggc accattcatt gcatggcgca aataaccacc actgccaaat
     5101 acgcttcccc acggcgcatt ccaggtcaat tgtccactaa ccaacgccgt ttcattgggc
     5161 tggctggtat tacgacttaa accttcgagc tgcgcgtaaa agccgagcaa ctcccctttc
     5221 aacgtataat tcgggtcgag cggtatgctt ttctcgagaa tactttgtat ctgattggaa
     5281 acatctttct gtaccgatgc ccccagagaa tagcgactgg tatcatcatg ccaactggcg
     5341 gctaaaaaag aacgtgcgcg atgtttatcg tattttcccg tctggctgtt atacacccct
     5401 tcatcacgaa taccggttgt tgaatcagtc cataaaaata ctcgtgaacg ccacattccc
     5461 cacagatcaa tatttggtgt taaagcccaa taacctaatg ctccttcaga aactgtcggt
     5521 aatgtacgtt gcgaactgcc agccattaac ggaatacgat aaggtaagct accataacta
     5581 taatccgtcc cgaagcgacc tagtttaagc aggctatttt cactgggttt tatttttact
     5641 gcccccaggg caaagccaaa acttttatca ccatcaagat accaccgggt actcatatca
     5701 gcacgagcac ctaatttata aacataatat gccccgcctt caacgccaat aatattggaa
     5761 taataaccac tattgaaatc gagcaatcct ccctgtaccc atgcgtaaat atctcttccc
     5821 gcgccagcac tgggcttatc cgccctgcgc aattcgtttt tcagtctgat gcgtagtccc
     5881 gcttcatcgt cagccagttg cgccgcataa gcagaggtaa ggccagaggc agccgtcagg
     5941 caaataacgg ccatggccac tatttttctc atagttgcac ctttgatgtt
//
LOCUS       DAZGLJ010000259         5913 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000259 DAZGLJ010000000
VERSION     DAZGLJ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.259"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(227..850)
                     /gene="tetR(Y)"
                     /locus_tag="ACHK49_004386"
     CDS             complement(227..850)
                     /gene="tetR(Y)"
                     /locus_tag="ACHK49_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(Y)"
                     /protein_id="HFO9264903.1"
                     /translation="MTKLDRETVIQAALELLNEVGVDNLTTRKLAERLKVQQPALYWH
                     FRNKRALLDALSEAMLEKNHTRTVPQTGEDWRVFLKENALSFRSALLSYRDGARIHAG
                     TRPTSAQYERVEKQIRFLCESGFEQPDAVRALVIVSHYTTGSVSEQQAALEDSSERKQ
                     ASKEAPAQPSQFLSHAFDTFDAEGADFAFEYGLDALISGLEMKKATK"
     gene            933..2108
                     /gene="tet(Y)"
                     /locus_tag="ACHK49_004387"
     CDS             933..2108
                     /gene="tet(Y)"
                     /locus_tag="ACHK49_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(Y)"
                     /protein_id="HFO9264904.1"
                     /translation="MSKSLITALIVVALDAIGLGLIMPVVPALLNEFVPAEQTAFHYG
                     VFLSLYAFMQVFCAPVLGRLSDRYGRRIILLVSFLGATIDYSIMAAAPVLWVLYIGRI
                     ISGVTGATGAIAASIIADTTKQEERARWFGFMGACFGAGMIAGPAIGGVLGDISVHAP
                     FVAGALLNAIAFCLVAFLLPKTPSQPPEGQPAKINLFEGFRFNFAVQGLASFFALFFL
                     MQLIGQAPAALWVIYGEQRLNWDIGTAGVSLAVFGAAHTFVQAVLTGTLSKRLGDRGV
                     LLLGMGADMCGFLLLAFITQSWMVLPAIFMLATGGIGMPALQAIISGLVCDEKQGALQ
                     GTLTGLTNITSIIGPVGFTTLYGLTAGQWDGWVWLVAASLYLIAIPLLRQSASLLRS"
     gene            complement(2124..2390)
                     /locus_tag="ACHK49_004388"
     CDS             complement(2124..2390)
                     /locus_tag="ACHK49_004388"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264905.1"
                     /translation="MASPPGNWKTRYKASDLADHQRLEMTCKKCNRLVYINKAMISSI
                     RGHEQLYLDEVERRARCKAQNCRGTMRMAMVRLDELSGFIGGIA"
     gene            complement(2558..3394)
                     /locus_tag="ACHK49_004389"
     CDS             complement(2558..3394)
                     /locus_tag="ACHK49_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFO9264906.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3394..4197)
                     /locus_tag="ACHK49_004390"
                     /note="aph(3'')-Ib"
     CDS             complement(3394..4197)
                     /locus_tag="ACHK49_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFO9264907.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(4258..5073)
                     /gene="sul2"
                     /locus_tag="ACHK49_004391"
     CDS             complement(4258..5073)
                     /gene="sul2"
                     /locus_tag="ACHK49_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFO9264908.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(5320..>5913)
                     /locus_tag="ACHK49_004392"
     CDS             complement(5320..>5913)
                     /locus_tag="ACHK49_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal sequence protein"
                     /protein_id="HFO9264909.1"
                     /translation="EVIIQENNRMLKRRDAFLKKSALAVSVALLLSAQAQAVLTGPVD
                     ANSSSLLIGENSFITNSTGTANNTFLLGGGAFNMDSPGSLQFGSFSGVYNSPHSVTLG
                     RDAGQAESKYGVAIGKSAEVLNSQQSVAIGGWAGIENSSGSVALGHGSQVSGENNVVS
                     VGAGPEGYGESVKGAPETHSIVNKTFLSFIFNGLFSC"
BASE COUNT         1390 a         1497 c         1525 g         1501 t
ORIGIN      
        1 ttcccgtttc agctgcgttt cctgtttggg gtcgtttgcg gtacgtgcaa tccgcagcga
       61 agatgcggat atactgccat ttaaatggct tggttatggt ttggttgcag ggttctttgt
      121 cgtctattca acacgcaaac caaaagctgc accaaaagca taatctttaa gttaatcatt
      181 ttaaaagcct cgctttgcgg ggctttttta tgttgcatct gctaagttat tttgtggctt
      241 tcttcatttc cagaccggaa attaaagcat ccaatccata ttcaaaggca aaatcggcac
      301 cttccgcatc gaatgtatca aaggcatgag atagaaactg tgatggctgt gcaggggctt
      361 cttttgaggc ttgtttgcgt tcagaactat cttcgagggc tgcttgctgc tcactgacag
      421 agcccgtcgt ataatgactg acaatcacta atgcccgtac ggcatcgggc tgttcaaatc
      481 cgctctcaca taaaaatctg atctgttttt ccacgcgctc atattgcgca ctggttgggc
      541 gtgtgcccgc atgaatgcgt gcgccatcac gataagaaag cagtgcgcta cgaaagctga
      601 gtgcattttc ttttaaaaag acacgccagt cttctcctgt ctgaggcacg gtgcgggtgt
      661 gatttttttc taacatggct tcagataagg catcaagcag ggcgcgctta ttgcgaaaat
      721 gccaataaag agcaggttgc tgcaccttca ggcgctcggc caattttcgc gttgttaaat
      781 tgtcaacacc gacttcgttg agaagttcaa gtgcagcctg aataacggtc tcacgatcca
      841 atttggtcat tttattgcat tacctcttga caatttatcg ttgataaagt ttattatcgc
      901 caacttatca atgataaata aaagtcacat ctatgtcaaa atcacttata accgcactca
      961 ttgttgtcgc gcttgatgcg attggtttgg gattaatcat gccggtggtt ccggctttat
     1021 taaatgaatt tgtaccggca gagcaaacag catttcacta tggtgttttt ttatcgcttt
     1081 atgcgtttat gcaggtcttt tgcgcgcccg ttttagggcg gttatctgac cgctatggac
     1141 ggcggattat tttgctggtt tcatttttag gtgccacgat tgattatagc ataatggcgg
     1201 cagcgcctgt tttatgggtg ctttatatcg gccggattat ctcaggtgtt accggagcaa
     1261 ctggtgcaat cgcggcatca attatcgctg atacaactaa acaggaagaa cgtgcgcgtt
     1321 ggtttggttt tatgggggcg tgttttggtg caggtatgat tgcagggcct gctattggcg
     1381 gtgttcttgg tgatatatct gtgcatgcgc cctttgtggc aggggctctt ctcaatgcaa
     1441 ttgccttttg tttggtggct ttcttgttgc ccaaaacgcc gtcacaaccg cctgaaggac
     1501 agccagccaa aatcaatttg tttgaaggct ttcgcttcaa ttttgcagtt cagggacttg
     1561 ccagcttttt tgcgttgttt tttcttatgc agctgattgg gcaggcgccc gccgctttgt
     1621 gggtgattta tggcgaacag cgcttgaatt gggatattgg cacagcaggt gtgtcgctgg
     1681 ccgtttttgg tgcagcacat acattcgtac aagctgtttt aaccggcact ctttcaaagc
     1741 gactgggtga ccgcggtgtg ttgctgcttg gaatgggcgc tgatatgtgc gggtttttat
     1801 tgctggcttt tatcacgcaa agctggatgg ttctgccggc aatttttatg ctggccacag
     1861 gcggcattgg tatgcctgct ttgcaggcca ttatttcagg tcttgtttgc gatgaaaaac
     1921 aaggtgcttt acagggaact ttaacggggt tgacgaatat aacctcgatt atcgggccgg
     1981 tcggatttac gacgctttat ggcttaaccg cggggcagtg ggatggttgg gtttggctcg
     2041 tcgcagcaag cctttatctt attgctatac cattattgcg ccagtcagcc agtttattgc
     2101 gatcttaaaa tcttggcggg agatcaagca atcccgccaa taaaaccgct gagttcatca
     2161 agacgcacca ttgccatgcg catggtgcca cggcaatttt gagctttgca gcgtgcgcgt
     2221 cgttcaactt cgtccaaata taattgctcg tgtcctcgaa ttgagctgat cattgcctta
     2281 ttgatataaa caaggcggtt acatttcttg caggtcattt ctaatctttg atggtctgca
     2341 agatcactgg ctttatagcg ggttttccag tttccgggtg gggatgccat tttcatcacc
     2401 agaagtcttc aggtgaggga atacggacat agctgatttt accgcccaca tggggtcgtt
     2461 tgcgggagag ggcgaaatcc tacgctaagg ctttggccaa cgatattctc cggtaagatt
     2521 gatgtgttcc caggggatag gagaagtcgc ttgatatcta gtatgacgtc tgtcgcacct
     2581 gcttgatcgc ggccgcgata gctagatcgc gttgctcctc ttctccatcc gcgttccaag
     2641 ctgcggaaag gcacccataa gcgtacgcct ggtcgagcag gcgacgcgga tcgacgtcca
     2701 gcgcacgaga gaatgcgtcc gccatctgtg caatgcgtct aggatcgaga caaaggtcgt
     2761 ctctgtcagc cggatcgtag aacatattgg cggcgccaaa gcccacttca ccgaccagac
     2821 cgacgggatc tatcaccagc cagccgcgac tggagaacat gatgttttca tgatgcagat
     2881 cgccatgtag cccacgcagt tccgaggcat tgctcatcat ttgatcggct ataatcgccg
     2941 cgtggacgta gtcagtttga caacctgcgt tttgatcatc gcgcgcccgc tgaaacaaag
     3001 ctgcaaagcg atcccggatc gggagaaggg cagaaggcag gggttcctca gatgcggcat
     3061 acagcttcgc cattagttcc gctgcaattt cggtcgcctg gtagtcgccg tgctcggcaa
     3121 cgatgtgaga gagcattcgc tccccggcat attcgagcaa catcagattg ttctcacgac
     3181 cgagcaaccg gactgctccc ctcccattgc gccataccag atagtcggcc ccgcgcagtt
     3241 catcagcaat gtcttctata ggtttcaatc ccttgacgat tgcaggagtc ccgtctggca
     3301 atgaaacttt ccaaacgagg ctggaaaagg tgtccgcaat gagaacaggt tgcgaaacgt
     3361 gccaatgagc aggaaaaaca ggcggcatga acatcaaccc caagtcagag ggtccaatcg
     3421 cagatagaag gcaaggcgtt cgcggtcggg ggcttcgatc cccaatacat tgaataggac
     3481 agcgaaggcg cgctctgctt catctggcgc tgcccagttc tcttcggcgt tagcaatcat
     3541 gagtgccaaa tcggcatagc gatctgctgt tccgagccgc ccaaggtcga tcagacccgt
     3601 gcattgaaga gttttagggt ccaccatgaa gttcggcatg cagggatcac catggcaaac
     3661 aaccatatcg gtgcgctctt ggtcgagccg caccggtagc tctcgttcga cacgagccaa
     3721 aagatcgagc tgcggcgtac tcttgtcctc gtccggtaag aagtcgggat tgacggcatt
     3781 gcgggacacc acatcaacgg cgcgtccgaa cattcgcgac agcctgcgct caaacggaca
     3841 ttgatcaacc gataggctgt gaacagcgcc aagttgctgc cccattgacg gccacgcttt
     3901 gagcaaatcc gctccagaca gatcagccgc cggtactccc ggaattgccg ttatcaccaa
     3961 gcatgcaccc tcctgttcct cctgccagtt gatgacctcg gggcaagcca cacctcgacc
     4021 tttgagccaa atgaggcggt cacgctctcc agcgagctca ccgcggcggg aagcaggtgc
     4081 gattttcgcg aaggcatgcc cgtcaccacg tcgaaaaaca aaatcaccag attctccgcc
     4141 tctgacaggc aaccagtcag aatgcgattc accaaaaaaa atattagttc gattcaatgg
     4201 aggttccttc agttttctga tgaagcgcga atatagagaa atatcccgaa tgtgcagtta
     4261 acgaattctt gcggtttctt tcagcgccgc caataccgcc agcccgtcgc gcaaggggcg
     4321 cggctcgtgt gtgcggatga agtcagctcc acctgcggcg gcggcaagct ctgcagcgag
     4381 tgtcgcggcc ccgacatccc ccggaccacg gcctgtgagc gcgcgcagaa aggatttgcg
     4441 cgaaacagac agaagcaccg gcaaatcgaa gcgcagccgc aattcatcga accgcgccag
     4501 caccgagagc gaggtttcgg gagcagcccc cagaaaaaac cccatgccgg gatcaaggac
     4561 aaggcggttg cgtttgatac cggcacccgt cagcgccgcg atgcgcgcgt caaagaacgc
     4621 cgcaatgtga tccatgatgt cgccagcggg tgcctcgcgc cgatctgcct gcccgtcttg
     4681 caccgaatgc ataacgacga gtttggcaga tgatttcgcc aattgcggat agaacgcagc
     4741 gtctggaaaa ccgcgaatat cattgagata ggccacacca cgcgacaagg cataggcttg
     4801 cgtcgcgggt tgataactgt cgagcgagac gggaatgcca tctgccttga gcgcgtccag
     4861 caccggcgcg atacgcgcga tttctgtgtc ggacgaaaca ggcgcggcgt cgggattgct
     4921 ggatgccgga ccgaggtcga tcacatctgc cccctcggcc atcagcttac gcgcctgcgc
     4981 aatggctgcg tctggcgcca gataccggcc tccatcggag aaactgtccg aggttatgtt
     5041 gacgatgccg aaaatgatga gcgatttatt catgggggct tctataataa taataataat
     5101 aatcgagcat gagtctcata cggatgctcg ggtcgaaagg gaatccccag gcgagtaacc
     5161 tgtttgcggt gatccattag ctgcaggagc agaatagcat acatctggaa gcaaagccag
     5221 gaaagcggcc tatggagctg tgcggcagcg ctcagtaggc aatttttcaa aatattgtta
     5281 agccttttct gagcatggta tttttcatgg tattaccaat tagcaggaaa ataagccatt
     5341 gaatataaaa gataaaaatg tcttgtttac aatagagtgg gtttccggtg ctcctttgac
     5401 actctctcca tatccctcag gcccggcacc aacagatacc acgttatttt ctcccgacac
     5461 ctgtgagcca tggcccagag ctacagagcc tgaagaattt tctattccag cccatccccc
     5521 aatagcaaca ctctgctgtg aattaagtac ctcagcgctc ttacctatgg caacaccata
     5581 ttttgactca gcctgaccgg catcccttcc cagggtgaca ctgtgtggag aattatacac
     5641 tcccgaaaaa gagccaaact gaagacttcc tggtgaatcc atgttaaaag cgccacctcc
     5701 caaaaggaaa gtgttattag ctgtaccagt tgaattcgta ataaaagagt tttctccaat
     5761 cagtaagctg gaagaattag catcaacagg ccctgtcaaa acagcctgag cctgagccga
     5821 gagcagcagg gccacactca ctgccagcgc gcttttcttt aaaaaagcat cacgtctttt
     5881 aagcattctg ttattctcct gtattattac ttc
//
LOCUS       DAZGLJ010000260         5895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000260 DAZGLJ010000000
VERSION     DAZGLJ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.260"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..385
                     /locus_tag="ACHK49_004393"
     CDS             <1..385
                     /locus_tag="ACHK49_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO9264910.1"
                     /translation="LIDSKLFCTWLTNTFLEMVICTGSNMANVNFNNANLSNCHFNCS
                     ILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFFEEDCTSHT
                     GMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(539..2188)
                     /locus_tag="ACHK49_004394"
     CDS             complement(539..2188)
                     /locus_tag="ACHK49_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO9264911.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2340..3689)
                     /gene="ghxP"
                     /locus_tag="ACHK49_004395"
                     /gene_synonym="yjcD"
     CDS             complement(2340..3689)
                     /gene="ghxP"
                     /locus_tag="ACHK49_004395"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO9264912.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4235..4699)
                     /gene="soxR"
                     /locus_tag="ACHK49_004396"
     CDS             complement(4235..4699)
                     /gene="soxR"
                     /locus_tag="ACHK49_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO9264913.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4785..5108
                     /gene="soxS"
                     /locus_tag="ACHK49_004397"
     CDS             4785..5108
                     /gene="soxS"
                     /locus_tag="ACHK49_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO9264914.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5111..>5895)
                     /gene="pdeC"
                     /locus_tag="ACHK49_004398"
     CDS             complement(5111..>5895)
                     /gene="pdeC"
                     /locus_tag="ACHK49_004398"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO9264915.1"
                     /translation="LSPKRKLQRALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQ
                     GQIMNPAEFIPLAEKEGMIEQITDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTS
                     RLIARINQKTEQYAVRPQQIKFEVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGY
                     SNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQ
                     VNWLRKRGVRYCQGCFFAKAMPPQVFMQWMEQLPARELTRGQ"
BASE COUNT         1598 a         1610 c         1387 g         1300 t
ORIGIN      
        1 cttaattgat agcaaactct tttgtacgtg gctaacaaat acattcctcg aaatggttat
       61 atgtaccggc tctaatatgg ctaatgttaa ttttaataat gccaatttaa gcaactgcca
      121 tttcaactgt tctattttaa caaaagcctg tatgtttaat acccgtctct atcgggttaa
      181 ttttgatgag gctagcgtcc agggaatggg catttctatt ctccgtgggg aggaaaatat
      241 ccccattgat agtgataccc tggtaacact acagaaattc tttgaagaag attgtacctc
      301 tcatactggc atgtcacaaa ctgaggataa tattaatgca gtcgctatga agattactgc
      361 agatattatg caacacgcag attgaggtta agtcgaagaa cagccaatga attgacttta
      421 caagagagat aacttatttg ttttagaatc atgaatgtta gcccgagcca acctgttaaa
      481 ggtgcaaaag attgcacctt tttcagatta cctcgggcct ggttacgtcg ctttcggctt
      541 actgattttc ctcaattagc aacgcttcaa gcaaatcgag atcgtgcagt aatttttgca
      601 gcgtttcgtt gctgatctcc cgcgtggcgc gcaggtggta cagttcagca cgttcagaac
      661 gcaatgccgc cagacggaag cgacgctcaa ggttctcttc ctgcacggaa ctttcaacgt
      721 cgttacgtcc atcggcgcga cgacgcaggt taccaatgac gcgagaactg acctccgtaa
      781 gcagctggtt atcgatattc tcttcggtat ccgccgccag acgctcctcc attttctgga
      841 tcgccacaat cgccacttct gccgttgccg ctcgcgcaat acgttcttct ttcaattgtt
      901 gcgaatggtc cgcgacttca atgtgttgta gcagaatagg caacatcacc acgccgacaa
      961 acagcgagaa gagaatgacg ccagccgcca ggaacaccag ctcatagcgc gccgggaaga
     1021 cgttaccatc cggcaagagc agcgggatag agagcacacc ggccagagtg atcgccccac
     1081 gcaccccggc gaaagacgcg atcaggattt ctcgtgtggt ccacgaacca aactccatcg
     1141 gcttcttctt caggaagcgg ttgctgaact ttttcatcgt ccacagccag ccgaaacgga
     1201 ccagcatcag cgccgcatat atcagaataa tatcggtaaa cagcatccag atttcgacgt
     1261 tagggtcgat ttctgccgcc atcagcgacg tctccagaat acccggcagc tgcagaccta
     1321 acagcaggaa caccatgccg ttaaagacaa attccagcat cgcccaggtg ctgtttgcgc
     1381 gcaggcgcat tgccagcggc gcacggcgca tcacaccgga gcgggtgatg gtcatcccgg
     1441 cggcaaccgc agcgaggatg ccggaaacgc caatatgttc ggcaatcaga taagaagcga
     1501 atggcagcaa gaacagcaga acgatctgcg tcgcgggttc atcaccgccc cagcggctga
     1561 ggaatcgcag cgagcgacca tacagccagc tcaccacaaa gccggcgaga ataccgccaa
     1621 tggctacttt cataaattcg accgtcgcac cgccaacggt gaagatcatc gtccccatcg
     1681 ccactgccac ggcaaacttc aacgacacca ggccggaggc gtcattcatc aacgcttcgc
     1741 cctgcaaaat acccatgatt tttttcggga tgcgcccttc gccaacaatc ccggagagcg
     1801 ccacagcatc ggtcggagaa agcactgccg ccagcgcaaa ggcggggatc agcggaatgc
     1861 ccggcaccac ccagtaaata aggaagccaa tgccgaccac ggtgaccacc accagcgcca
     1921 gcgcgaggcc aaaaatctct cgaccatgtt cgagaaattc acgggtcggc gttttccagc
     1981 catcagcgaa cagcaacggc gggataaaca agactaaaaa gagttcagga tcaaactcca
     2041 catgcaaacc aaacgtcggc cacgccagta gcgcaccgat ggcgatttgc ataagcggaa
     2101 gcgggatctg aaagggcatg acacgagtga ccaccccgga cagcgagacc acgagggtca
     2161 ttatcagtat ggtgaagaag atttccatgc gttccctgtt tgcgatgttt ttttatgatc
     2221 tgagaaagac gttatgcctg aattctcatt ttttcagaat atcgcaaaag ctcacacaat
     2281 gtgcggagaa aataaaaacg ggcagccatt gccacccgtt ttcagaatcc gatgaaagat
     2341 tagatagccc agccacccgc atagaaggtc accagcgcca cggcgataac gaccgtaccg
     2401 atgttcaact tgcgccattc gccggaaacc agacgaccaa tcaccagagt cgcgaagccg
     2461 atcatgatgc ctgttacgat gttacaggtc agcacgatga ataccgccgt aaccagaccc
     2521 gccatcgcat caacaaaatc agcaaagtcg attttcgcca cgttgctcag catcagcagg
     2581 ccaacgtaca tcagcgccgg agccgttgca taccccggaa cgaggtaaga gagcggagag
     2641 aggaacagaa tcagcaggaa cagcacgcca acggtgatag ccgtcaaacc ggttttaccg
     2701 cccgccgccg tacccgccgc agactcgatg tataccgctg ccggagcagc acccaccagg
     2761 ccagagaaaa cgctgctcat ggagtcagtg gtcagtgctt tcccaccgtc gatgatctgc
     2821 ccatctttat ccagcaggtt cgcctggccg gcgacggcac ggatagttcc ggtggcatca
     2881 aatactgccg tcatcaccag cgccagaacg cttggcagga ctacaggatt cagcgcgccc
     2941 ataatgtcca ggctgccaat cagtgaattg ccgttttcat cgctcaatga aggcatggcg
     3001 aaaacgccgg agaaatggac gttaggatcg aagatcaaac cgacaattga gataccgata
     3061 atggtcagca gaatgccacc agggactttc agtttttcca ggccgatgat caccgccaga
     3121 cctactagtg acataatcac cgggaaggtc gcgaaatcac ccagcgcaac gggcagacca
     3181 tcaagcgggt ttttaatcac cagaccgaca ccattagcgg caatgagcag caggaacaga
     3241 ccgataccga tccccgtgcc gtgcgccaca ccgtgcggca agttgcgcaa aatccagcta
     3301 cggatacccg tggcagaaat taccgtaaac agcaccccca tcaggaacac ggcacccagc
     3361 gcgacaggta cgctaatatg ttgccccagc accaggctga atgcggtaaa cgccgtcagg
     3421 gagatggcgc aaccaatcgc caacggcaga ttagcccaca gacccatcac gatagaaccg
     3481 agtccggcaa ccagacaggt tgcaacgaaa actgccgcag gcgggaagcc tgctttaccc
     3541 aacatacctg gaacgacgat gaccgagtag accatcgcca gaaacgttgt taacccggca
     3601 accacttcct gacggacagt gcttccacgt tgtgaaattt taaaccaggc gtcgagtgaa
     3661 ccgccggtac gcgctgatgg cgtagacata agaaaacatc ccctgagagt ttaattttcg
     3721 tcaagatgcg tggcggacaa tcccctgcca gcgaaacgtt atcttcctgc tccaggtttg
     3781 cgacaattta ctgcgtcgca aaaaagcaaa cgtttagctc cgcgcataca aaacgggtgg
     3841 taaaaaaagc aaacgattat ccggcgatca tagcggcttt ttcaaccgaa gtttgctgct
     3901 tttttctctt ttattgtctt gacgatgaaa aaccaggcaa ttgatgcgca aacgttatcg
     3961 tcggtgcgca agattgcagc gttatcgagc cgtataccgt tcaggattgc gataaaaaat
     4021 cgtctgagat gacaaaaata ttgcggataa aaagtatact tttacactgg cgaagaaatg
     4081 ccggtgacat acaattaaag catcaacacc aaccggaacc tccaccacgt gctcgaatgg
     4141 ggtgtgttga cgtcggggga aaccctcctg tgtaccagcg ggatagagag aaagacaaag
     4201 accggaaaac aaactaaagc gcccttgtgg cgctttagtt ttgttcatct tccagcaagc
     4261 gtgcgccggt accttcttct cctaagcggt cgcccgggtt acgcaacggg caatcactgc
     4321 gcgaaaggca gccacaacca atacatccgt ccagttcgtc acgcagcgct actaaggtat
     4381 gaatgcgccg atccaactct tctcgccatt gggacgaaag ctgtttccac tctttcgcac
     4441 ttaacgtatg cccttcgggc aacacgccaa acgcttcacc aatggtcgcc agcggaatgc
     4501 caatacgctg agcaattttg ataattgcaa catatcgcaa cacatcacgt ttatatcgcc
     4561 gctgattgcc gctgttacgg atactggtaa tcaacccttt actttcatag aaatgcagcg
     4621 ccgataccgc cacaccgctg cgtttcgcca cttcgccggg ggttagcagc gctttaatgc
     4681 ggggtaattt cttttccata aatcgcttta cctcaagtta acttgaggaa ttatactccc
     4741 caacagatga attaacgaac tgaacactga aaagaggcag atttatgtcc catcagaaaa
     4801 ttattcagga tcttatcgca tggattgacg agcatattga ccagccgctt aacattgatg
     4861 tagtcgcaaa aaaatcaggc tattcaaagt ggtacttgca acgaatgttc cgcacggtga
     4921 cgcatcagac gcttggcgat tacattcgcc aacgtcgcct gttactggcc gccgttgagt
     4981 tgcgcaccac cgagcgtccg atttttgata tcgcaatgga cctgggttat gtctcgcagc
     5041 agaccttctc ccgcgttttc cgtcggcagt ttgatcgcac tcccagcgat tatcgccacc
     5101 gcctgtagtt ttattgcccg cgcgttaact cccgcgcggg taattgctcc atccattgca
     5161 taaacacctg cggcggcatc gccttcgcaa aaaaacatcc ctggcaatag cgcacgccgc
     5221 gtttgcgcag ccagttaacc tgctcctcag tttcgacgcc ttcagcgatc gtttttaacc
     5281 ccaggctgtg cgccagctcg atgatgtgtt ccgcaatcaa atgactggtt ttgtgggtgg
     5341 tcagcgtttc aacaaacgat ttatcgattt tcaaaatatc gacattcaat gatttaaggt
     5401 tatgcaagtt agagtagcca ataccaaaat catcaattgc cacttcgtaa ccagcctggc
     5461 ggaaagccag aataatcggc gtcattttgt caacatcaag aaatgcatgt tcagtcactt
     5521 caaatttaat ttgctgcgga cgcaccgcgt attgctctgt tttctgattg attcgcgcta
     5581 tcaaccgtga cgtatgaaaa tcggaggccg acaggttaat agaaacatag cgatcggcat
     5641 gtgttgccaa gtacgcgccc agatcacgga agacattatc aataacataa tcagttatct
     5701 gttctatcat cccctccttt tctgccagcg gaataaactc tgccggattc attatttgcc
     5761 cctgctcacc aagccaacgt aacaacgctt cagcgccgat acatttttct gttttgatat
     5821 cgattattgg ctggtaataa agacaaagtt gatgtttttc gagggcgcgt tgcaatttac
     5881 gtttgggaga taaat
//
LOCUS       DAZGLJ010000261         5823 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000261 DAZGLJ010000000
VERSION     DAZGLJ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.261"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            205..4167
                     /gene="putA"
                     /locus_tag="ACHK49_004399"
     CDS             205..4167
                     /gene="putA"
                     /locus_tag="ACHK49_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO9264916.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPLSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(4207..4845)
                     /gene="rutR"
                     /locus_tag="ACHK49_004400"
     CDS             complement(4207..4845)
                     /gene="rutR"
                     /locus_tag="ACHK49_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO9264917.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            5133..>5823
                     /locus_tag="ACHK49_004401"
     CDS             5133..>5823
                     /locus_tag="ACHK49_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFO9264918.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQ"
BASE COUNT         1318 a         1571 c         1594 g         1340 t
ORIGIN      
        1 acctttcaaa aatgttaact gccgcagaga aaaagtctga gttatttttt taatccctgt
       61 catatcgatt tcttttatta acatttcatt catttttaag cttgctacgc atgtcacatt
      121 taacatggtt gcacaaagtt gcaacatcat ggatatttca cgataacgtt aagttgcacc
      181 tttcagaaca acaggagtaa tggcatggga accaccacca tgggggttaa gctggacgac
      241 gcgacgcgtg agcgtattaa gtctgccgcg acacgtatcg atcgcacgcc acactggtta
      301 attaagcagg cgattttttc ttatctcgaa caactggaaa acagcgatac tctgccggag
      361 ctacctgcgc tgctttctgg cgctgccaat gagagcgatg aagcaccgac tccggcagag
      421 gaaccacacc aaccattcct cgactttgcc gagcaaattt tgcccctgtc ggtttcccgc
      481 gccgcgatca cagcggccta tcgccgcccg gaaaccgaag cggtttctat gctgctggaa
      541 caagcccgcc tgccgcagcc agttgctgaa caggcgcaca aactggcgta tcaactggcc
      601 gataaactgc gtaatcaaaa aaacgccagt ggtcgcgcag gtatggttca ggggttattg
      661 caggagtttt cgttgtcatc gcaggaaggc gtggcgctga tgtgtctggc ggaagcgttg
      721 ttgcgtattc ccgacaaagc cacccgtgat gcgttaattc gcgacaaaat cagcaacggt
      781 aactggcaat cacacattgg tcgtagcccg tctttgtttg ttaatgccgc cacctggggg
      841 ctgctgttta ctggcaaact ggtttccacc cataacgaag ccagcctctc ccgctcgctg
      901 aaccgcatta tcggtaaaag cggtgaaccg ctgatccgca aaggtgtgga tatggcgatg
      961 cgcctgatgg gcgagcagtt cgtcactggc gaaaccatcg cggaagcgtt agccaatgcc
     1021 cgcaagctgg aagagaaagg tttccgttac tcttacgata tgcttggcga agctgcactg
     1081 actgccgcag atgcacaagc gtatatggtt tcctatcagc aggcgattca cgccattggt
     1141 aaagcgtcca atggtcgtgg catctatgaa ggtccgggca tttcaatcaa gctgtcggcg
     1201 ctgcatccgc gttacagccg cgcccaatat gaccgggtaa tggaagagct ttacccacgt
     1261 ctgaaatcac tcaccctgct ggcgcgtcag tacgatattg gtatcaacat tgacgccgaa
     1321 gaggccgatc gtctggagat ctccctcgat ctgctggaaa aactctgttt cgagcctgag
     1381 ctggcaggct ggaacggtat cggttttgtt attcaggctt atcaaaaacg ctgcccactg
     1441 gtgattgatt acctgattga tctcgccacc cgcagccgtc gccgtctgat gattcgcctg
     1501 gtgaaaggcg cgtactggga tagtgaaatc aagcgtgcgc agatggacgg ccttgaaggc
     1561 tatccggttt atacccgcaa ggtgtatacc gacgtttcat atctcgcctg tgcgaaaaag
     1621 ctgctggcgg tgccgaatct aatctacccg cagttcgcga cgcacaacgc ccatacgctg
     1681 gcagcgattt atcaactggc aggtcagaac tactatccgg gtcagtacga gttccagtgc
     1741 ctgcatggta tgggcgagcc actgtatgag caggtcaccg ggaaagttgc cgacggcaaa
     1801 cttaaccgtc cgtgtcgtat ttatgctccg gtcggtaccc atgaaacgct gttggcgtat
     1861 ctggtgcgtc gcctgctgga aaacggtgct aacacctcgt ttgttaaccg tattgccgac
     1921 acctctttgc cactggatga actggtcgcc gatccggtca ctgctgtaga aaaactggcg
     1981 caacaggaag ggcaaactgg attaccgcat ccgaaaattc ccctgccgcg cgatctttac
     2041 ggtcacgggc gcgacaactc ggcagggctg gatctcgcta acgaacaccg gctggcctcg
     2101 ctctcttctg ccctgctcaa tagtgcactg caaaaatggc aggccttgcc aatgctggaa
     2161 caaccggtag cggcaggtga gatgtcgccc gttattaacc ctgcggaacc gaaagatatt
     2221 gtgggctttg tgcgtgaagc cacgccgcgt gaggtagaac aggcgctgga aagtgcggtt
     2281 aataacgcgc caatctggtt tgccactcct ccggttgaac gcgcggcgat tttgcatcgc
     2341 gctgccgtgc taatggaaag ccagatgcag caactgattg gtattctggt gcgtgaagcc
     2401 ggaaaaacct tcagtaacgc cattgccgaa gtgcgcgaag cggtcgattt tctccactac
     2461 tatgccggac aggtgcggga tgatttcgct aacgaaaccc accgtccatt agggcctgtg
     2521 gtgtgtatca gtccgtggaa cttcccgctg gctattttca ccgggcagat tgctgccgca
     2581 ctggcggcag gtaacagcgt gctggcaaaa ccggcagaac aaacgccgct gattgccgcg
     2641 caagggatcg ccattttgct ggaagcgggt gtaccgccag gcgtggtgca gttgctgcca
     2701 ggtcaggggg aaaccgtggg cgcgcaactg acgggtgatg atcgcgtacg cggggtgatg
     2761 tttaccggtt caaccgaagt cgctacgtta ctgcagcgca atatcgccag ccgcctggac
     2821 gctcagggtc gccctattcc gctcatcgct gaaaccggcg gcatgaacgc gatgattgtc
     2881 gattcttcag cactgaccga acaggtcgtc gtggatgtac tggcctcggc gttcgacagt
     2941 gcgggtcagc gttgttcggc gctgcgcgtg ctgtgcctgc aagatgagat tgccgaccac
     3001 acgttgaaaa tgctgcgcgg cgcaatggcc gaatgccgga tgggtaatcc gggtcgcctg
     3061 accaccgata tcggtccggt gattgatagc gaagcgaaag ccaatattga gcgccatatt
     3121 cagaccatgc gtagcaaagg ccgtccggtg ttccaggcgg tgcgggaaaa cagcgaagat
     3181 gcccgtgaat ggcaaagcgg cacctttgtc gccccgacgc tgatcgaact ggatgacttt
     3241 gccgaattgc aaaaagaggt ctttggtccg gtgctgcatg tggtgcgtta caaccgtaac
     3301 cagctaccag agctgatcga gcagattaac gcttccggct atggtctgac gcttggtgtc
     3361 catacgcgta ttgatgaaac catcgcccag gtcactggct cggcccatgt tggtaacctg
     3421 tatgttaacc gtaatatggt gggcgcagtg gtgggtgtgc agccattcgg cggcgaaggg
     3481 ttgtccggta ccgggccgaa agcaggcggt ccgctctatc tctaccgtct gctggcgaat
     3541 cgcccggaaa gtgcgctggc agtgacgctc gcgcgtcagg atgcagagta tccggtcgat
     3601 gcacagttga aagccgcatt gactcagccg ctaaatgcac tgcgggaatg ggcagcaaat
     3661 cgtccagaat tgcaggcgtt atgtacgcaa tatggcgagc tggcgcaggc aggaacacaa
     3721 cgattgctgc cggggccgac gggtgaacgc aacacctgga cgctgctgcc gcgtgagcgc
     3781 gtgttgtgta ttgccgatga tgagcaggat gcgctgactc agctcgccgc cgtgctggcg
     3841 gtgggcagcc aggtactgtg gccggatgac gcgctgcatc gtcagctagt aaaggcattg
     3901 ccatcggcag tcagcgaacg tattcaactg gcgaaagcgg aaaatataac cgctcaaccc
     3961 tttgatgcgg tgatcttcca cggtgattcg gatcagcttc gcgcattgtg tgaagccgtt
     4021 gctgcgcggg atggcgcaat tgtttcggtg cagggttttg cccgtggcga aagcaatatc
     4081 cttctggaac ggctgtatat cgaacgttcg ctgagtgtga ataccgctgc cgctggcggt
     4141 aacgccagtt taatgactat aggttaaaca ggtagccgga ggatgttaca acctcctccg
     4201 gcatctttaa cgtggtcgaa tcccctcaat aataatccgc tgcacgtttt caaccgtttg
     4261 attgaaaaat atctcatcgc gcaacgtcgc gcctgtcacc gcctccacct gaggggcgaa
     4321 atcggcgtaa tgttgagtgg aagcccaaat catgaaaatc aaatgctgcg gatcaatcgg
     4381 cgcgagtttg ccgcttttga cccaaccggc aatcagcgcc gatttctcat caattaatgc
     4441 cttcaaatcg cccgtcagtt catccattaa cagcggcgcg cctgccagca tctccataca
     4501 gaacaggcgc gaagcctgcg gataatcgcg ggagacttcc agcttcagac ggatgtactc
     4561 ttttatcgcc gccagcggag cgaaatcttc acgaaacgct tttaacggtg ccagccagat
     4621 atcgagaatc tgccgcagca cggcaatata cagcgcctct tttgacggaa agtaatacag
     4681 cagattggtt tttgaaacac ccgccaactc tgcgatctgc tccagccttg tgccgtgaaa
     4741 accgaattgt gaaaaagtgt ccagtgctgc gctaagaatc gcttttttct tcgcgcttac
     4801 tgcgcgcgaa cgtttacccg ttgttttcac tgcgccttgc gtcatgcgct ctcccctctt
     4861 gtagattggc aacaggatag caaaaccccc gcccggcctc gaccttctgc actctcatcg
     4921 cgcacagatg catgttttat gtgcaactgt tttgaccgtt tagtccactt tttaccagat
     4981 attttagaca acacaatgtt aacacattaa taacattcac tttttaaaaa ctggcatccg
     5041 ctttgcaaac aagccattac gcggctgaaa aggatatgca ggatgcagcg ccccgtctga
     5101 ctttcacttt acgagacgaa gagaggttga tgatgaaaat tggcgtattc gtacctattg
     5161 gcaacaacgg ctggctcatt tcgacccacg cgccgcagta catgccgacc tttgaactga
     5221 ataaagccat tgtgcaaaaa gcggagcact accatttcga tttcgccctg tcgatgatca
     5281 aactgcgtgg ctttggcggc aaaactgagt tctgggatca caaccttgag tcgttcacct
     5341 tgatggcggg gctggcggcc gtcacctcgc gcattcagat ttacgccact gctgccacct
     5401 taacgttacc tccggcaatc gtcgcccgta tggccgcaac catcgactcc atctctggcg
     5461 ggcgttttgg cgtcaacctc gtgactggct ggcaaaagcc cgagtatgag cagatgggta
     5521 tctggcctgg cgatgactat ttctcccgtc gttacgacta tctcaccgaa tatgttcagg
     5581 tgctgcgcga cctgtggggc acggggaaaa gcgattttaa aggcgatttt ttcaccatga
     5641 atgattgtcg cgtcagtccg caaccgagtg tccccatgaa agtgatctgc gccgggcaaa
     5701 gcgacgctgg catggcgttc tccgcccagt atgccgattt caacttctgt ttcggcaaag
     5761 gcgtaaatac gcccacggct ttcgccccga ccgctgcgcg gatgaaacag gccgcagagc
     5821 aaa
//
LOCUS       DAZGLJ010000262         5810 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000262 DAZGLJ010000000
VERSION     DAZGLJ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.262"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            148..594
                     /gene="hpaR"
                     /locus_tag="ACHK49_004402"
     CDS             148..594
                     /gene="hpaR"
                     /locus_tag="ACHK49_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFO9264919.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            713..2377
                     /gene="tsr"
                     /locus_tag="ACHK49_004403"
     CDS             713..2377
                     /gene="tsr"
                     /locus_tag="ACHK49_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO9264920.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMAVADSGENWETF"
     gene            complement(2420..3691)
                     /locus_tag="ACHK49_004404"
     CDS             complement(2420..3691)
                     /locus_tag="ACHK49_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO9264921.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEVVFSLGQA
                     WPALFLIFAVVGGIRANIFTPTEAGAAAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLTLVTYIPAISLFLV"
     gene            complement(3688..4161)
                     /locus_tag="ACHK49_004405"
     CDS             complement(3688..4161)
                     /locus_tag="ACHK49_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO9264922.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVMTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKLRRVIAVLVAVLSIALLLYMAWLG
                     YRLAEMAQFKVTQLLKVSWFWIDLAVPVGALATAIYTLYWLIHDIKQSDSGDKMQ"
     gene            complement(4225..5196)
                     /locus_tag="ACHK49_004406"
     CDS             complement(4225..5196)
                     /locus_tag="ACHK49_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HFO9264923.1"
                     /translation="MSKIIAMLITVSTLFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFSTLSPEQLEMIHQTGYEAGVYSQKLTLERDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
BASE COUNT         1569 a         1337 c         1456 g         1448 t
ORIGIN      
        1 aaagataata aaatattaaa tatcagaaag ttaataaaac atgctcaatt ttgataaaat
       61 gaagaattgc aggggcagcg cagtcccggt aacgtgatga ttaactgttt ctatattaat
      121 gattttgaat ttttcagggg aaagataatg cacgactctc taaccattgc gttgctccag
      181 gcgcgagaag cggcgatgag ttattttcgc ccaatcgtta aacggcataa tttgactgag
      241 caacagtggc gcatcgtgcg tattctggcg gaaagcccat caatggattt tcacgatctg
      301 gcgtatcgcg cctgcatttt gcgcccaagc ctgaccggga tcctcacgcg gatggaacgc
      361 gacggtttag tgttgcgatt aaagccgatc aatgatcagc ggaagctcta tatctcgctg
      421 accaaagagg ggcaggcgct gtataaccgc gctcagacac agattgaaga agcctatcgg
      481 cagattgaag ctcagtttac tgccgagaaa atgcaacagt taacccatct gttagaggaa
      541 tttattgctc tgggtaattc ccgacaagaa gacatcccgg gcgataatga ataacttttt
      601 agtatctgat aaccagcgcc tggtttttcc atggatggcg ggttacacct tttcataaag
      661 tttttgcttt ccaggccgaa aatcttgcat cggtccacag gaaagagaaa ccatgttaaa
      721 acgtatcaaa attgtgacca gcttactgct ggttttggcc gtttttggcc ttttacaact
      781 gacatcaggc ggtctgttct ttaatgcctt aaagaatgac aaagaaaatt tcactgtttt
      841 acaaaccatt cgccagcagc aatccacgct gaatggcagc tgggtcgcgt tgttgcagac
      901 gcgtaacacc ctcaaccgcg cgggtatccg ctacatgatg gatcagaata atattggtag
      961 cggttcaacc gttgctgagc tgatgcagag tgccagtatt tcgctgaaac aggcggaaaa
     1021 aaactgggcg gattacgaag cgttgccgcg tgacccgcgt cagagcaccg ccgcagcggc
     1081 agagatcaaa cgtaattacg atatttatca caatgcgctg gcggagctga tccaactatt
     1141 aggtgcaggc aaaatcaacg agttctttga tcagccgacc cagggatatc aggacggttt
     1201 cgagaagcag tatgtggctt acatggagca aaacgatcgg ctctatgata tcgccgtcag
     1261 cgataacaat gcctcctaca gccaggcgat gtggattctg gtgggcgtga tgatcgtcgt
     1321 actggcggtc atcttcgccg tctggttcgg tattaaagcc tcgctggtag cgccaatgaa
     1381 tcgcctgatt gacagcattc gtcatattgc aggcggcgat ctggtgaaac cgattgaggt
     1441 ggatggctct aatgagatgg ggcaactggc agagagtttg cgccatatgc agggagagct
     1501 gatgcgtacc gtcggtgatg tgcgcaacgg ggccaatgcc atctatagcg gtgccagcga
     1561 aatcgctacc ggcaataacg atctctcttc gcgcaccgag caacaggccg cttcgctgga
     1621 agagacggca gccagcatgg agcaactgac cgcaacggtg aaacagaacg ccgagaatgc
     1681 gcgccaggcc agccatctgg cattaagtgc ttctgaaacg gcgcaacgcg gcggcaaagt
     1741 ggtagataac gtggtgcaga ctatgcgcga tatctccacc agttcgcaga aaatcgccga
     1801 tattatcagc gtaattgacg gcattgcctt ccagaccaat attctggctt tgaacgcggc
     1861 ggttgaggct gcgcgtgcgg gtgagcaagg acgcggtttt gcggtggtcg cgggagaagt
     1921 gcgtaatctg gcccagcgta gcgctcaggc ggctcgtgaa attaaaagcc tgattgaaga
     1981 ctcggtggga aaagtggatg ttggctctac gctggtcgaa agcgccgggg aaacaatggc
     2041 ggagattgtc agtgctgtga cccgcgtgac ggacattatg ggcgaaattg cttctgcttc
     2101 tgatgagcag agccgtggta tcgatcaggt tggcttagcg gttgctgaga tggaccgggt
     2161 aactcaacag aacgccgcgc tggtggaaga atctgccgcc gccgccgccg cgctggaaga
     2221 gcaggccagt cgcctgaccg aagctgtggc agtgttccgg attcagcaac agcagcaaca
     2281 gcagcgtgaa acatcggctg tggtaaaaac cgtgacgcca gctgcgccgc gtaaaatggc
     2341 cgtggcagat agcggggaga actgggaaac gttttaacgc aatgacgccg gatgagtatt
     2401 tcatccggcg tttttattat taaaccagga acagtgaaat cgccgggata tatgtcacca
     2461 gcgttagcat taatagtaga gccgccagta gaggcaggga ttgtttaaag aaatttccca
     2521 ccgaaacttg cgtgatgtta cataccagca gcatcacggt acctaccggt ggcgtgaatg
     2581 cgccaatcga caggttgaag atcatgatga tgccaaaatg caccggatcg atgccaaagt
     2641 gttcgaccac cggttttaat agtggtacca ggatcagcaa aatggcgttt ccttcaagaa
     2701 acatccccag cgtcagcagc aatatgttga taattaacag gaacatatat ttattttcgg
     2761 tcagcgaagt cacaaaattc gccactgcga caccagcatg ttccattgag aaaatccatc
     2821 ccaacgcggc gctggccatg attaccagca tcaccgatgc ggttgcgcgt gccgtttcgc
     2881 ccaatgcttt gacgacatga gaaatccgca tttcacgata gataaaaaag ccaatcgcca
     2941 gcacgattag caccgctgct gcacctgctt ctgttggggt gaaaatgtta gcgcgaatac
     3001 cacccaccac agcaaaaatg aggaacagcg caggccaggc ctgtcccagc gagaacacca
     3061 cctctttgcc cgtaggccag ctttcacgcg agggtttagc gttagttttg cgggcgacca
     3121 ggtagacggt aaacatcaga acgactgcgc atagcaaacc aggtaccagc ccagcgataa
     3181 acatttttcc gatagagacg tcggcaacca gaccgtaaat aatcagggct attcccggcg
     3241 gtatgatcgg ggtgactaac gagccaccag cggtaatggc ggcggcaaac gcgcggttgt
     3301 agcctttacg ctccatctcc ggcaccatca tgcgcgacag catcgccgcg tcagcaaggt
     3361 tagaggcgct gacgccgccc atcatggtgc tgacgagaat gtttgccacg ccaagtccgc
     3421 cgcgcatttt ccccaccaga acgttggcaa tgtgcaggat gcgttcggca atgccggtgt
     3481 agctcattag tgtgccgagc aagataaaaa acggaattgc cagcaatgat gggctttgcg
     3541 ttggcgcgat cagtctttgc acggcaattt caatcggtac gctggagaag aataaaaagt
     3601 aagcgatgat gccaataaac atcgccagat agacccgaat gttgagaaac aaacagacga
     3661 tcattagcgg aataatcaat aaccaactca ttgcattttg tctccgctat cagattgttt
     3721 tatatcgtgt attaaccagt agagggtata aatagccgtt gccagtgcac cgacagggac
     3781 cgccagatcg atccagaacc aggaaacctt cagaagttgt gtcactttaa actgcgccat
     3841 ttcggctagt cggtagccca gccaggccat atacagcagc agtgcgatgg agagaacggc
     3901 gaccagtact gcaataacac gcctgagctt gcctggaagc atctccatca ggaagggaat
     3961 ggtcaggtgc gccctgtcac gttccgccac cactccacct aacatcacca cccaaatcat
     4021 cagcattccc gacatttctt ctgtccattg aagcggttgg ccgatgaaat aacgcataaa
     4081 taccgctcca attgtcatca caaccagaac gaatagcgca aaggcagcac taaattctgc
     4141 cactttgcca aaaagacgca tgaaacctcc tgttagtgcg tgtcctgcct aagagagatg
     4201 aggcaggacg ctgagcatta atgcttattg cagttgctgt tgaatggttt cgtacagacc
     4261 tggtgtccat tccgggaatt gagagtaaac ttcgcgtgct ttcttctgga atggtccagt
     4321 atcgggatag atcacttcga cgcccgctgc ctgcatggtt ttcaacattt cggcatcacg
     4381 ttcaagagtg agtttctggc tatagacacc cgcttcatag ccggtctggt ggatcatctc
     4441 cagttgttct ggtgaaaggg tgctaaagaa ggcttcacct cctatccata ctgaggtatt
     4501 tgtcaggtag ttaaccattg tcagatatct tgcctgctca tacagttttt gcccttgcag
     4561 cactgaaatt gggttttcta cgccgtcaat aacgccctgg gttaatgcgg gataaacttc
     4621 gcctaatggc atgggagttg gtgttgcccc catcgcctga atggctttta tttgcatgac
     4681 gttattagga acacgaattt ttaagcctgc gagatcgtca acagtgcgaa tgggtttttt
     4741 cgaaataatc tgtcgagtgc catagagata attattcatt accacatgaa ttcccttttt
     4801 cttcagactt tcattttttt gtttaaacca gtcgctttca tatattttga acagtttttg
     4861 cggatcgtca gtcagatacg gtccaaacaa aatacccaaa tcgggttcat aatcggcaag
     4921 aaaggccaca tcactcaagg ttatgacatt catgcccatc atggcttgct cggtgacatc
     4981 ctgttttgag cccaattggg aacttggata gagcgcaagt gttatttcgc cattactttt
     5041 tttatttaat aggtctgccc agtaacgcat gaccacatcc aggggttctc cagggttatt
     5101 ttcataggca acttttatcg aaataggttt tgcctggatt attgacggta aaaaaagggt
     5161 actgacagta attagcatcg ctattatttt gctcatcttt acttactcca tgctaaggaa
     5221 ataaaactcc aacattgttg tcgggagtga aaccttatga aaaagcagtc attactgaaa
     5281 agtagtcagc gaatagcgga atggctgcaa atgtgtcata aattcgtgca ccgggtaata
     5341 ctttgctgct tttaatcggt ttttaatgca ttttttgatt gtatatctta aagttatgta
     5401 atgtatgacg ttacaaggaa cacccactga tgagtttagt taccgctgat aagatctctt
     5461 attcggttga tgatttatta aaataaaagc attaatttgg ctgcggctat ttttacagat
     5521 aaaaactaag tgttgacgca gaatggtggt atccattaaa tttatttagt gctgatttaa
     5581 tacatttgct gaacgtataa ttcatgctaa cgttaaaaga aagccatcat tcactaaata
     5641 acttgttcgg caaggagatc aaagagtaac ggatgattcg ttacaacggt taaaggtagt
     5701 aaatattcac atggtgaatt tgttatcaac tggaaaactc atctcactgg tttcctcctg
     5761 agcatgtgag tgaacgctgt gattaatgat taacgttaag tgctaaccag
//
LOCUS       DAZGLJ010000263         5797 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000263 DAZGLJ010000000
VERSION     DAZGLJ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.263"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            127..2559
                     /gene="hsdR"
                     /locus_tag="ACHK49_004407"
     CDS             127..2559
                     /gene="hsdR"
                     /locus_tag="ACHK49_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFO9264924.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKAYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2626..4095
                     /locus_tag="ACHK49_004408"
     CDS             2626..4095
                     /locus_tag="ACHK49_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO9264925.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     VLELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMKTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRIENEQAWKVSIDDVIARNFNLDIKNPHQAETI
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4095..>5797
                     /locus_tag="ACHK49_004409"
     CDS             4095..>5797
                     /locus_tag="ACHK49_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO9264926.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASELLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWTRLINLGIWALGSGFPNVVQGSTDKEILMCKVSDMNLEGNEKFIFSTKNTISK
                     DLADEYKIKISEPGTIIFPKIGGAIATNKRRILVQDTAIDNNCLGIKPCDAISGEWFY
                     LILNTLDMSKYQSGTSIPAINQSVIGSIPIALPSLKMQEKIVSYVITLMSLCDQLEQQ
                     SLTSLDAHQQLVETLLGTLTDSQNAEELAENWARISEHFDTLFTTEASVDALKQTILQ
                     LAVMGKLVPQDPNDEPASELLKRIAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEG
                     WEWCKFGLTSEFINGDRGSNYPNKNEYVSQGIPWINTGHIEKNGTLTVTEMNFITEGK
                     FNELRSGKIQKGDLVYCLRGATFGKTAFVIPYETGAIASSLMIIRPFITEMGGYIYNY
                     LTSPFGRSQIYRFDNGSAQPNLSANSVMLYSFPCPPLTEQYRIFSQVGLLHELCDKLK
                     TR"
BASE COUNT         1745 a         1355 c         1373 g         1324 t
ORIGIN      
        1 tactattgat gaaactcaag aataccttta aaacagtgac ttaacgctga taatactctt
       61 tgaacagatg aattcatcta tttttctgtg tagaatttgc gcatcgcatt tagatgagaa
      121 caccccatgg cggaactgaa cctaagtaac ctgacggaag cagacatcat taccaaatgc
      181 gttatgccag ccattctcaa tgcaggctgg gacaacacaa cgcaaatcag acaggaggtc
      241 aaactccggg acggtaaagt catcgtacgt ggtaaagttg cggcacgcag aacggtaaaa
      301 tctgctgaca tcgtgctgta tcacaaacca ggcattccct tagccgtgat tgaagcaaaa
      361 gccaacaaac atgaaattgg caaagggatg caacagggca ttgaatatgc gcgcctgctg
      421 gacgttcctt ttgtttttgc caccaacggg gatggcttta tcttccgcga tgccaccgca
      481 gccgaaggtg aatgccttga aaagcaaata acgctggatg acttcccctc ccccgctgaa
      541 ctctggcaaa aattctgcct ctggaaagga tacacacaag ctcagcttcc ggtgattact
      601 caggactatt acgacgatgg cagcggtaaa tcgccacgtt attaccagct tcaggcaatc
      661 aacaaaacca ttgaagccgt ctccaacggg caaaaccgcg ttctgctggt catggcgacc
      721 ggaacaggga aaacctatac cgcattccag atcatctggc gcctgtggaa atcaaaaaat
      781 aaaaaacgca ttttgttcct tgccgatcgc aacattctgg tcgaccaaac caaaaataat
      841 gatttccagc catttggtac ggcaatgacc aaagtcagcg gacgcaccat tgatcccgct
      901 tatgaaattc acctcgcgct ctatcaggct ataactggcc cggaggaaga ccaaaaagcg
      961 tttaaacaag tcgcaccaga tttcttcgat ctgatcgtga tcgacgaatg ccatcgcggc
     1021 agcgcatccg aagacagcgc ctggcgagag atccttgatt acttcagttc cgccacccaa
     1081 attggcttaa ccgccacgcc aaaagagacg catgaagtct ccagcacgga ttacttcggc
     1141 gatccggttt acgtctactc gctaaaagaa gggatcgaag acggcttcct cgccccttat
     1201 aaagttgtcc gtgttgatat tgatgttgat ctgcaaggct ggcgcccaac caaagggcaa
     1261 actgacttaa acggcgaagt gatcgacgat cgtatctata accagaaaga tttcgatcgc
     1321 acgatggtaa tcgacgaacg gacagaactg gttgccagaa ccattaccga ctacctcaag
     1381 cgcaccaacc cgatggataa aaccatcgtc ttctgtaacg acatcgatca tgcagaacgt
     1441 atgcgccgcg ccctggtgaa tctcaacccg gagcaggtga aaaagaacga caaatacgtc
     1501 atgaaaatca ccggcgatga tgaaattggc aaagctcagt tggataactt catcaacccg
     1561 aaaaaagcgt acccggttat cgcgaccact tcagagctga tgaccactgg tgtggatgct
     1621 aaaacctgca aactggtagt actggaccag aacatccagt cgatgaccaa attcaagcaa
     1681 attatcggtc gtggtacacg catcgacgaa cgttacggca aactctggtt taccatcctc
     1741 gactttaaaa aagccaccga actgtttgcc gatgagcgtt tcgatggcat tcccgaaaaa
     1801 gtcatggata ccacaccaga ggatatcgcc gatccagaat ctgattttga agagaaactc
     1861 gaagaaatca gcgaacatga cgacgaacag gtaacaggcg ttgatgaacc gcctgcgcca
     1921 ccataccagg ttaccgatac cgatgatgtc ggcccacttc cggaagaaga cgagaagaaa
     1981 atccgcaagt ttcacgtcaa cggtgtagcg gtgggcgtta ttgcgcagcg tgttcagtat
     2041 tacgatgccg acggtaaact ggttaccgaa tcttttaaag attacacccg caaaacactg
     2101 ctcaaagaat atgcctcgct ggatgacttt acccgcaagt ggcaggacgc cgatcgcaaa
     2161 gaagcgatca ttcacgagct ggagcaacag gggatcatct gggaagtact ggcagaagaa
     2221 gtcggtaaag atctcgaccc gttcgacatg ctttgccacg tagtgtatgg tcagccgccg
     2281 ttaacccgca aagagcgcgc cgagaacgtg cgcaagcgga actacttcac aaaatactct
     2341 gaagcagcgc aagccgtgct cgataatctg ctggataaat acgccgatgc gggcgtacag
     2401 gagatcgaaa gtattcaggt gctgaaactt aaaccattcg acagcatggg cactttaccg
     2461 gagattatta aaaccggatt tggcgaccgt aacgggtata atcaggcgct cagcgagctg
     2521 gaaaacgaaa tctaccaatt accgccccgc tctgcttaac aactctcttt tgtgccttct
     2581 gcaaccaaca tgttgcagaa ggccttgttt ttcatggaat acaacatgtc tatcagttca
     2641 gtaatcaaat cattacaaga tattatgcgc aaagatgccg gtgtggatgg cgatgcgcag
     2701 cgtctcggtc agctctcctg gctgctgttt ttgaaaatct tcgatgccca ggaagaggtg
     2761 ctggaactgg agcaggataa ctatcaatat ccgatcccac agcgttattt atggcgcagt
     2821 tgggccgcaa acgctcaggg cattaccggt gattccctgc tggaattcgt taatgatgat
     2881 ctgttcccgg cgttgaaaaa cctcactgcg cctatcgata aaaacccacg cggctacgtg
     2941 gtgaagcagg cgttcagcga tgcctataac tatatgaaaa acggtacgct actgcgccag
     3001 gtgatcaaca agctgaacga aattgacttt accagcgcca gcgaacgcca tctgtttggt
     3061 gatatttacg aacagatcct taaagatctg caatctgcgg gcaatgctgg cgaattctat
     3121 acgccacgcg ccgtcactcg ctttatggtg gatcgcgttg atccgaaact cggcgaatcc
     3181 attatggacc cggcctgcgg tacgggcggt tttcttgcct gcgcatttga tcatgtaaag
     3241 aacaaatacg tgaagagcgt cgccgatcat cagacgctgc aacaacagat ccacggtgtt
     3301 gagaaaaaac agcttccgca cctgctggcg accaccaata tgctgctgca cggcattgaa
     3361 gtaccggtac aaattcgtca cgacaacacc ctgaacaaac cgctttcctc ctgggatgaa
     3421 caactggatg tcattgttac caacccgccg tttggtggca cggaagaaga cggtattgag
     3481 aagaactttc cggcagagat gaaaacccgc gaaacggcgg atctgttcct gcaactgatt
     3541 gtggaagtac tggcgaaaaa cggtcgcgcg gcggtggtat tgccggatgg cacactattt
     3601 ggcgaaggcg ttaaaaccaa aatcaaaaaa ctgcttaccg aagagtgcaa cctgcatacc
     3661 atcgtgcgtt taccgaatgg ggtgtttaac ccctataccg gcattaaaac caacctgttg
     3721 ttctttacca aaggtcagcc aaccaaagag atttggttct atgagcatcc gtatccggcg
     3781 ggcgtaaaaa actacagcaa aaccaagccg atgaagtttg aagagtttca ggcggagatc
     3841 gactggtggg gtaacgaggc cgatggtttt gccagccgca tcgagaatga gcaagcgtgg
     3901 aaagtcagca ttgatgacgt gattgcgcgt aacttcaatc tggatattaa aaacccacat
     3961 caggcggaaa ccattagcca tgatccggac gaactgttag cgcagtatgc aaaacagcag
     4021 gcggagatcc agacgctgcg taatcaactg cgcgatattc ttggcgctgc gctgtctggc
     4081 aaggaggtta actaatgagt gtggaaaagc tgatcgttga tcatatggaa acctggacct
     4141 cggcgttgca aacccgttcc accgccgggc gcggcagctc cggtaagatt gatttgtatg
     4201 gtattaagaa attgcgtgag ctgattctgg aactggcggt gcgaggtaaa ctggtgccgc
     4261 aggatccgaa cgatgaaccg gcgtcggagc tgctgaagcg tattgcggcg gaaaaagcag
     4321 agctggtgaa gcaaggtaaa attaaaaagc aaaaaccgct gccggaaatt agcgaggaag
     4381 agaagccgtt tgaattgccg gaggggtggg aatggactcg attaattaat cttggtatat
     4441 gggctttagg gtctggtttt ccaaatgtgg tacagggaag cactgataaa gaaattttga
     4501 tgtgcaaagt tagcgacatg aatcttgaag gtaacgagaa attcattttt tcgacaaaaa
     4561 atacaatttc aaaagaccta gctgatgaat ataaaattaa aatctcagaa cccggcacca
     4621 tcattttccc taaaattggc ggagcaattg caactaataa acgaagaatt ttagttcagg
     4681 atacagctat tgataacaac tgtcttggaa taaaaccatg tgatgcaata tcgggggagt
     4741 ggttctatct aatccttaat actctcgata tgtcgaagta tcaatcagga acctcaatac
     4801 ccgcgataaa tcaaagtgtc ataggtagta taccaattgc gcttccttct ttaaaaatgc
     4861 aagagaagat tgtttcttat gtcataaccc taatgtccct ttgcgaccaa ctggaacagc
     4921 aatccctgac cagtctggac gcacatcagc aactggttga aaccctgttg ggaacactta
     4981 cagacagcca aaacgccgag gaactggctg aaaactgggc gcgtattagc gagcatttcg
     5041 acaccctatt taccaccgaa gccagcgtgg atgcgttaaa acagaccatt ctgcaactgg
     5101 ccgtaatggg caaacttgtg ccgcaggatc cgaatgacga accagcctct gaactgctca
     5161 aacgaattgc gcaggaaaaa gctcaactgg tgaaagaagg aaaaataaaa aaacaaaaac
     5221 cgttgccgcc aattagcgat gaggaaaaac cgtttgaact tccggaaggg tgggagtggt
     5281 gtaaatttgg tttaacatca gagtttataa atggcgatcg tggaagtaat taccccaata
     5341 aaaatgagta tgtttctcaa ggtattcctt ggatcaatac tggacatata gaaaaaaatg
     5401 gtacacttac ggttacagaa atgaatttta taaccgaagg aaaatttaat gaattaagaa
     5461 gtgggaaaat tcaaaaaggt gatttggttt attgcctacg aggagcaact tttggtaaaa
     5521 ctgcctttgt aatcccctat gaaacaggag ctattgcctc atcactaatg atcattagac
     5581 catttatcac agaaatggga gggtatattt ataattattt aaccagtcca tttgggcgct
     5641 cacagattta ccgctttgat aatggctcag cacaacccaa cctatcagca aatagtgtaa
     5701 tgttgtattc attcccatgc ccaccattaa ctgaacaata tagaatattt tcacaagtag
     5761 gtttacttca tgaactatgc gataagctta aaactcg
//
LOCUS       DAZGLJ010000264         5796 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000264 DAZGLJ010000000
VERSION     DAZGLJ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.264"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            9..305
                     /locus_tag="ACHK49_004410"
     CDS             9..305
                     /locus_tag="ACHK49_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO9264927.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            311..1096
                     /gene="bet"
                     /locus_tag="ACHK49_004411"
     CDS             311..1096
                     /gene="bet"
                     /locus_tag="ACHK49_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO9264928.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            1093..1770
                     /locus_tag="ACHK49_004412"
     CDS             1093..1770
                     /locus_tag="ACHK49_004412"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO9264929.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEIHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVYGEQW"
     gene            1770..1952
                     /locus_tag="ACHK49_004413"
     CDS             1770..1952
                     /locus_tag="ACHK49_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO9264930.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            1925..2116
                     /locus_tag="ACHK49_004414"
     CDS             1925..2116
                     /locus_tag="ACHK49_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO9264931.1"
                     /translation="MHKASPVELRTSIGMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMAAKAEANERDPA"
     gene            2193..2408
                     /locus_tag="ACHK49_004415"
     CDS             2193..2408
                     /locus_tag="ACHK49_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO9264932.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            2507..2728
                     /locus_tag="ACHK49_004416"
     CDS             2507..2728
                     /locus_tag="ACHK49_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO9264933.1"
                     /translation="MADIIDSASEIEELQRNTAIKMHRLNHQAISATHCCECGDPIDE
                     LRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            2725..3030
                     /locus_tag="ACHK49_004417"
                     /pseudo
     CDS             2725..3030
                     /locus_tag="ACHK49_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            3327..3677
                     /locus_tag="ACHK49_004418"
     CDS             3327..3677
                     /locus_tag="ACHK49_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO9264934.1"
                     /translation="MINRTKLEHILEYARQQRRFGQLCKILPGDMVEIVEIAMRKTGN
                     SPVSPGGWISCSERMPAQDDWILIYSKHGEYMAGQVQEEYVELSDGTLSWLGNALYWM
                     LLPEPPQGVNDELA"
     gene            3752..3994
                     /locus_tag="ACHK49_004419"
     CDS             3752..3994
                     /locus_tag="ACHK49_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9264935.1"
                     /translation="MFALIQRGQIYTDSAGYPIKILRCINNTVLYRRMDGRTQSVKIN
                     DFNELFERIDHQEYRQILAETEQENHLKKLRAMQRR"
     gene            4113..4457
                     /locus_tag="ACHK49_004420"
     CDS             4113..4457
                     /locus_tag="ACHK49_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264936.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            4563..4781
                     /locus_tag="ACHK49_004421"
     CDS             4563..4781
                     /locus_tag="ACHK49_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO9264937.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTSSLLKRIRNGKKAKS"
     gene            4759..>5796
                     /locus_tag="ACHK49_004422"
     CDS             4759..>5796
                     /locus_tag="ACHK49_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9264938.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELLSGNRRESLIDRIKGADAITLHAWLDRYETILSERGIRPKTLLDYAS
                     KIRAIRRKLPDKPLADISTKEVAAMLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHV
                     ATNPVTATRTAKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCR
                     MKWSDINDNHLHIEQGKTGAKLAIPLTLTIDALNISLADTLQKCREASGSETIIASTH
                     HEPLSPKTVSKYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHK
                     SDSMAARYRDSR"
BASE COUNT         1672 a         1333 c         1509 g         1282 t
ORIGIN      
        1 gttcagccat gaacgcttat tacattcagg atcgtcttga ggctcagagc tgggcgcgtc
       61 actaccagca gatcgcccgt gaagagaaag aggcagaact ggcagacgac atggaaaaag
      121 gcctgcccca gcacctgttt gaatcgctat gcatcgatca tttgcaacgc cacggggcca
      181 gcaaaaaagc cattacccgt gcgtttgatg acgatgttga gtttcaggaa cgcatggcag
      241 aacacatccg gtacatggtt gaaaccattg ctcaccacca ggttgatatt gattcagagg
      301 tataaaacgg atgagtacag cactcgcaac gctggctggg aagctggctg aacgtgtcgg
      361 catggattct gtcgacccac aggaactgat caccactctt cgccagacgg catttaaagg
      421 tgatgccagc gatgcgcagt tcatcgcatt gctgatcgtc gccaaccagt acggccttaa
      481 tccgtggacg aaagaaattt acgccttccc tgataagcag aacggcatcg ttccggtggt
      541 gggcgttgat ggctggtccc gcatcatcaa cgaaaaccag cagtttgatg gcatggactt
      601 tgagcaggac aatgaatcct gcacatgccg gatttaccgc aaggaccgta atcatccgat
      661 ctgcgttacc gagtggatgg atgaatgccg ccgcgaacca ttcaaaaccc gcgaaggcag
      721 agaaatcacg gggccgtggc agtcgcatcc caaacggatg ttacgtcata aagccatgat
      781 tcagtgtgcc cgtctcgcct tcggatttgc tggtatctat gacaaggatg aagccgagcg
      841 cattgtcgaa aataccgcat acactgcaga acgtcagccg gaacgcgaca tcactccggt
      901 taacgatgaa accatgcagg aaattaacac tctgctgatc gccctggata aaacatggga
      961 tgacgactta ttgccgctct gttcccagat atttcgccgc gacattcgtg catcgtcaga
     1021 actgacacag gccgaagcag taaaagctct tggattcctg aaacaaaaag ccactgaaca
     1081 gaaggtggca gcatgacacc ggacattatc ctgcagcgta ctgggatcga cgtgagagct
     1141 gtcgaacagg gagatgatgc gtggcacaaa ttacggctcg gcgtcatcac agcttcagaa
     1201 attcacaacg taatagcaaa accccgatca ggaaagaagt ggcctgacat gaaaatgtcc
     1261 tacttccaca ccctgctggc tgaggtttgc accggtgtgg ctccggaagt taacgctaag
     1321 gctctggcct ggggaaaaca gtacgagaac gacgccagaa ccctctttga gttcacttcc
     1381 ggcgtgaatg ttactgaatc cccgatcatc tatcgcgacg aaagtatgcg caccgcctgc
     1441 tctcccgatg gtttatgcag tgacggcaac ggccttgagc tgaaatgccc gtttacctcc
     1501 cgggatttca tgaagttccg gctcggtggt ttcgaggcca taaaatcggc ttacatggcc
     1561 caggtgcagt acagcatgtg ggtgacacga aaagatgcct ggtactttgc caactatgac
     1621 ccgcgcatga agcgtgaagg cctgcattat gtcgtgattg agcggaatga aaagtacatg
     1681 gcgagttttg acgagatggt gccggagttc atcgaaaaaa tggacgaggc actggctgaa
     1741 attggttttg tatatgggga gcaatggtaa tgaagcatcc tcacgataat atccgggtag
     1801 gcacgatcac tttcgtctac tccgttacaa agcgaggctg ggtatttccc ggcctttctg
     1861 ttatccgaaa tccactgaaa gcacagcggc tggctgaaga gataaataat aaacgagggg
     1921 ctgtatgcac aaagcatctc ctgttgaatt aagaacgagt atcgggatgg cacatagcct
     1981 cgctcaaatt ggagtcaggt ttgtgccaat accagtagaa acagacgaag aatttcatac
     2041 gttagccaca tccctttcac aaaagctgga aatgatggcg gcgaaagcag aagcaaacga
     2101 gagagacccg gcatgacaac aacagaatgc atttttctgg cagcaggctt catattctgt
     2161 gtgcttatgc ttgccgacat gggacttgtt caatgacacc tcagcaggaa aacgcccttc
     2221 gcagcattgc ccgtcaggct aattctgaaa tcaaaaaagc cagacagcag tttccggata
     2281 aaaacgtcga tgacatttgc cgtagcgtac tgaagaagca ccgcgaaacg gtaacgctaa
     2341 tgggattcac accgactcat ttaagtctgg cgatcggcat gttaaacggc gtctttaagg
     2401 agcgatgaac atgaaaagca aaatcatcag ggagctacag gctccttttt tattgttcgc
     2461 attcaccctc aagcgtatta accaacaatt cagggattaa tggaagatgg cagacatcat
     2521 tgattcagca tcagaaattg aagaattaca gcgcaacaca gcaataaaaa tgcaccgcct
     2581 gaaccaccag gctatatctg ccactcattg ttgtgagtgt ggcgatccga tagatgaact
     2641 aagacgtctg gccgttcagg gttgtcggac ttgtgcaagt tgccaagagg atctggagct
     2701 tatcagtaaa cagagaggtt cgaagtgagc gaaattaact ctcaggcact gcgtgaagcg
     2761 gcagagcagg caatgcatga cgactgggga tttgatgcgg accttttcca tgagctggta
     2821 acaccatcga ttgtgctggc actgctggat gaacgggaaa gaaaccagca atacatcaaa
     2881 cgccgcgacc aggagaacga ggaaattgcg ctaacggtag ggaagctgcg tgttgagctt
     2941 gaggaagcaa aatcaaaact caacgagcag cgtgagtatt acgaaggtgt tatctcggat
     3001 gggagtaaac gtattgctga actggagaaa agcgaagagc aactcattaa cgagcgtgac
     3061 catgctgagt ctgctttagc tgatatgtac ttcgcaacaa ccggggatag gcctgagtgg
     3121 agtaactgtt tcagtttttc agatgctgtc gatgccgtag ttgacagaat tgctgattta
     3181 gaagccaaac agccatcgcc agtagtaccg gaagaaaaac caatgcctaa ccctcttagc
     3241 atgtacacag ttgatgctgt tgcagctatt gcagaggtga gaggctggaa cgcctgccgt
     3301 gcagccatgc ttaagggaga taaatcatga ttaatcgaac caaactggag cacatcctcg
     3361 agtatgccag gcagcagagg cgctttggcc agctttgtaa aattctgcca ggagatatgg
     3421 ttgaaatcgt ggagattgcc atgcgtaaga ctggcaactc tccggtaagt ccgggtggtt
     3481 ggataagctg tagtgagcga atgcccgctc aagatgattg gattttaatt tattcaaagc
     3541 acggtgagta tatggcagga caggtacaag aggaatacgt ggagttgagc gacggcactt
     3601 tatcgtggtt agggaacgcc ttgtactgga tgctgctacc agaaccgccg cagggagtga
     3661 atgatgaatt ggcctgaagc attcaccgct gtaggagttg caatcgcggt ggcatttatt
     3721 ctgtattcgc ttttccgctg gggataaagg aatgttcgct ctgattcaac gtggtcagat
     3781 atacaccgat agcgccggct acccgataaa aattcttcgc tgcataaaca acactgtgtt
     3841 gtacagaaga atggatgggc gaacacagtc ggtaaaaata aacgatttta atgaactgtt
     3901 tgaacggatc gatcaccagg aataccgaca aattctggct gaaacagagc aggagaacca
     3961 tctgaaaaaa ttacgcgcca tgcaaaggag ataaaccggt aaaggtgttc gcgataaagg
     4021 tgaatatcgg caatgaataa caatcctcgc actcgcgggg atttctttta tctgaactcg
     4081 ctacggcggg ttttgtttta tggagacaag aaatgtcaga tttggctatg aaggttttga
     4141 aatggcaatc gactggcgat gttggcatca gtagcgcaac tcttgcctca atcgcatgtg
     4201 gactgaaaaa gaatatctat ggtcatcact tcggcgctcc acatgacgca gcagacttcc
     4261 ggcgatgcgt tgcacttgtt gagcagattc cagaaatcag agattcattc gacaaggttg
     4321 caaagcgcgt tccggcattc aaaggcatcc tcaacgaatg ggattccctc gtcgctctgt
     4381 tgaagtctga aatgaagata cacggaaaca aagcaccaga gacttacaga agaattagcg
     4441 agctacgcaa ggactaacca cagcctcaca ctcaatgagg cctgttcatt tctcaagata
     4501 tccagaccta ccattgccgc atcaatgcgg cttttcttgc gtgtaattgc ggagactttg
     4561 cgatgtactt gacacttcag gagtggaatg cacgccagcg acgcccaaga agccttgaaa
     4621 cagttcgtcg atgggtgcgc gaatgcagga tattccctcc tccggttaag gatggaagag
     4681 agtatctgtt ccacgaatca gcggtaaagg ttgacttaaa tcgaccagta acaagtagcc
     4741 ttttgaagag gatcagaaat gggaagaagg cgaagtcatg agcgccggga tttaccccct
     4801 aacctttata taagaaacaa tggatattac tgctacaggg acccaaggac gggtaaagag
     4861 ttcggattag gccgagacag gaggatagca atcactgaag caatacaggc caatattgag
     4921 ttgctatccg ggaacaggcg tgagtcactg atagacagaa ttaaaggcgc tgacgcaatc
     4981 actcttcatg cgtggcttga ccgatatgaa acaatcctca gcgagagggg tatcaggccg
     5041 aaaactctac tcgactacgc cagcaaaatc agggcaatcc gaagaaaatt gccggacaaa
     5101 ccgctcgctg acatatcaac gaaagaggtg gcagcaatgc taaacaccta cgtagcagaa
     5161 ggtaaagcgg cttccgcaaa attaatcagg tcaacccttg ttgacgtttt tcgtgaggca
     5221 atagccgagg ggcatgtggc tacgaatccg gtaacagcaa cccgcacagc aaaatcagaa
     5281 gtaaggcgtt caagactgac agctaatgaa tatgtcgcga tttaccatgc agccgaaccg
     5341 ctcccaatct ggctgaggct ggcaatggat ttggctgtcg ttacagggca gagagtaggc
     5401 gatttatgca gaatgaaatg gtcagacata aacgacaacc atcttcacat tgagcagggt
     5461 aaaacagggg ctaagctcgc cattccgctg acgctaacga ttgacgcgct caatatctca
     5521 ttggctgata cactacagaa atgcagggag gccagcggca gtgaaacaat aattgcatca
     5581 acgcatcacg aaccgctttc cccgaaaaca gtatctaagt attttacaaa ggcgagaaat
     5641 gcatctggac tctcatttga tggagacccg ccaacattcc atgaactgcg cagcctgtcg
     5701 gcgaggctat accggaatca gattggcgat aagtttgctc aacgtcttct cgggcataaa
     5761 tcagattcaa tggcggcgcg ttacagggac agtcgc
//
LOCUS       DAZGLJ010000265         5790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000265 DAZGLJ010000000
VERSION     DAZGLJ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.265"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            13..1083
                     /gene="ygjJ"
                     /locus_tag="ACHK49_004423"
     CDS             13..1083
                     /gene="ygjJ"
                     /locus_tag="ACHK49_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO9264939.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            1100..3451
                     /gene="ygjK"
                     /locus_tag="ACHK49_004424"
                     /pseudo
     CDS             1100..3451
                     /gene="ygjK"
                     /locus_tag="ACHK49_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            3777..>5790
                     /gene="fadH"
                     /locus_tag="ACHK49_004425"
     CDS             3777..>5790
                     /gene="fadH"
                     /locus_tag="ACHK49_004425"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO9264940.1"
                     /translation="MSYPLLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVASSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHHVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALE"
BASE COUNT         1398 a         1571 c         1617 g         1204 t
ORIGIN      
        1 tggattaaca caatgaaatt aatcactgca ccatgcagag cattacttgc tctgccgttt
       61 tgctacgcct tttctgcggc aggagaagaa gcacgtccgg cagaacatga cgacacaaaa
      121 acacctgcaa ttacctcgac atcttctcct tcatttcgtt tttacggcga attaggggtt
      181 ggtggatata tggatttaga gggtgagaat aaacataaat acagcgacgg tacctatatt
      241 gaaggtggcc tggagatgaa gtacggctcc tggttcggcc tgatttacgg cgaaggctgg
      301 accgtgcagg ccgaccacga cggcaatgcc tgggtgccag accatagctg gggtggtttc
      361 gagggcggaa ttaaccgttt ctatggcggt tatcgtacca atgatggcac cgaaatcatg
      421 ctcagtctgc gtcaggattc ctcgctggat gacctgcaat ggtggggcga cttcaccccc
      481 gatctgggct acgtcattcc caatacccgc gacattatga ccgcgctgaa ggtgcagaac
      541 ttaagcggca actttcgtta tagcgtcacc gccacacctg ccggacatca tgacgaaagc
      601 aaagcctggc tacattttgg caagtacgat cgctatgacg acaaatacac ctatccggca
      661 atgatgaacg gttacatcca gtatgacctt gccgaaggca tcacctggat gaacggtctg
      721 gaaatcaccg acggcacagg acagctctat ctcacgggcc tgctaactcc taactttgcc
      781 gctcgcgcct ggcaccatac cggacgcgcc gacgggctgg acgtaccggg aagtgaaagt
      841 gggatgatgg tgagcgccat gtatgaagcg ttaaagggcg tttatctctc caccgcttac
      901 acctacgcca aacatcgccc tgaccacgct gacgatgaaa ccacctcttt catgcagttt
      961 ggtatctggt acgaatacgg cggcggacgt ttcgccacgg cttttgatag ccgcttctac
     1021 atgaaaaatg cctctcacga tcccagcgac caaatcttcc tgatgcaata tttctactgg
     1081 taataaggac tgtaatttca tgaaaataaa aactatttta acgccagtaa cctgcgctct
     1141 gctgataagt ttttccgccc atgccgctaa cgccgacaat tataaaaacg tgattaaccg
     1201 tactggcgcg ccgcagtaca tgaaggatta cgattacgac gatcaccagc gttttaatcc
     1261 gtttttcgat ctcggagcct ggcatggtca tctgttgcca gacggcccta acaccatggg
     1321 cggctttccg ggcgttgcgc tgctgacgga agagtacatc aactttatgg ccagcaattt
     1381 cgaccgcctg accgtctggc aggacggcaa gaaagtcgac tttacgctgg aggcatacag
     1441 tattcccggt gcgctggtgc aaaaactgac agcaaaagat gtgcaagtcg aaatgactct
     1501 gcgcttcgcc acgccgcgca cgtcactact ggaaaccaaa atcaccagca ataaaccgct
     1561 ggatctggtg tgggacggcg aactgctgga aaaactggaa gcgaaagaag ggaaaccgct
     1621 ttccgataaa accattgctg gcgaataccc tgactaccag cgcaaaatca gcgccacccg
     1681 tgatggcctg aaagtcacct ttggcaaagt gcgcgccacc tgggatctgc tgacctccgg
     1741 cgaatcggaa tatcaggtgc ataaatccct gccggtgcag actgaaatca acggcaatcg
     1801 ctttaccagt aaggcacata tcaacggttc gacgacgctc tataccacct attcccatct
     1861 gctgacctct caggaagtta gcaaagagca aatgcagatc cgcgatattc tggcgcgtcc
     1921 ggcgttttat ctcaccgcct cgcagcaacg ctgggaagag tatctgaaga aagggttaac
     1981 caatccggat gcgacgccgg aacagacgcg cgtcgcggtg aaagcgatcg aaacgctcaa
     2041 cggtaactgg cgctcaccgg gcggtgcggt gaaatttaac accgttacac cgtcggtgac
     2101 cgggcgctgg ttctccggca atcagacctg gccgtgggat acctggaagc aggcgtttgc
     2161 gatggcgcat ttcaatccgg acatcgccaa agagaatatc cgcgcggtct tctcctggca
     2221 gatccagcct ggcgatagcg tgcgtccgca ggatgtgggc tttgtccccg acctgatagc
     2281 gtggaacctt agccccgagc gtggtggcga tggcggcaac tggaacgaac gtaataccaa
     2341 acccagcctt gccgcctggt cggtgatgga agtgtacaac gtcacccagg ataaagcctg
     2401 gttggcagag atgtacccga aactggtggc ctatcacgac tggtggttac gtaaccgcga
     2461 tcacaacggc aacggcgtgc cgtaatatgg cgcgacccgc gacaaagccc acaacactga
     2521 gagcggcgag atgctgttta cggtgaagaa aggcaacaaa gaagagacgc agtctgggct
     2581 gaacaactac gcccgcgtgg tggagaaagg ccagtacgac agtctggaaa ttccggcaca
     2641 ggtcgctgcg tcgtgggaat cggggcgtga tgatgccgcc gtctttggtt ttatcgacaa
     2701 agaacagttg gataaatatg tcgctaatgg cggcaaacgt agcgactgga cggtgaaatt
     2761 cgccgaaaac cgcagtcagg acggaacgtt gctgggctac tcgctattgc aggagtcggt
     2821 ggatcaggcc agctatatgt acagcgataa ccattatctg gcggagatgg caaccatcct
     2881 cggtaagccg gaagaggcca aacgctatcg ccagttggca cagcagctcg cagactacat
     2941 aaacacctgt atgttcgacc cggctaccca gttctactat gacgtgcgta ttgaagataa
     3001 accgctggcg aacggctgcg cgggcaaacc gattgtcgag cgcggtaaag ggccggaagg
     3061 ctggtcgccg ctgtttaacg gtgcggcaac gcaggccaac gccgacgcgg tggtgaaggt
     3121 gatgctcgat cctaaagagt tcaacacctt tgtcccgctg ggaacggcgg cgttaaccaa
     3181 tccggctttt ggcgctgata tctactggcg cgggcgcgta tgggtggatc agttctggtt
     3241 tggtctgaaa gggatggagc gttacggtta tcgtgatgat gccctgaaac tggcggatac
     3301 gttcttccgt cacgccaaag gattaaccgc cgatggccca attcaggaga attacaaccc
     3361 gctgactggc gcacagcaag gcgcaccaaa tttctcctgg agtgccgcgc atttgtatat
     3421 gttgtataac gattttttcc gtaagcaata atttgcaact gctggcggat gcggcgtaaa
     3481 cgccttatcc gccctacatg tgcaatcctc ctggccggat aaaacgcgac aagcgtcgca
     3541 tccggcatct gcaccgtgcc actggcggat gcggcgtgaa cgccttatcc gccctacatg
     3601 tgcaatcccc caggccggat aaaacgcggc aagcgtcgca tccggcgtta ccaccgggcg
     3661 tattcttttt gaatcccatc acaaaccccg cactcccctt ttcccttttc tccggcgacg
     3721 gctaaattag aactcatccg accacataac aattatttta catactggac aattttatga
     3781 gctacccgtt gctgttcgcc ccgctggatt taggttttac cacgttaaaa aaccgcgtgt
     3841 tgatgggctc aatgcacacc gggctggagg aatacccgga cggtgccgag cggctggcag
     3901 cgttttatgc cgaacgcgcc cgtcacggcg tggcgctgat tgtcagcggc ggcattgcac
     3961 cagatttaac aggcgttggc atggaaggtg gcgcaatgct caacgacgcc agccagatcc
     4021 cacaccatcg caccattacc gaagcggtac atcaggaagg cggcaaaata gccctgcaaa
     4081 ttttgcatac cgggcgctac agctaccaac cgcatctggt cgcttcctcc gcattgcagg
     4141 cccccatcaa ccgtttcgtt cctcacgaac tcacccatga agagatcctg caactgatcg
     4201 acgatttcgc ccactgcgcg caactggcac gggaggcagg atacgacggt gtagaggtaa
     4261 tggggtccga agggtatttg atcaacgaat ttctgacgct gcgcaccaat cagcgtagtg
     4321 accagtgggg cggcgattac cgcaaccgga tgcgatttgc cgtagaagta gtgcgtgcgg
     4381 tgcgcgaacg cgtcggcaac gacttcatta ttatctaccg actgtcgatg ctcgacctgg
     4441 tcgaagacgg cgggactttt gccgaaacgg tagagctggc gcaggccatt gaagcggcgg
     4501 gcgcgaccat tatcaacacc ggcattggct ggcatgaagc acgtattccg accattgcca
     4561 cgcccgtgcc gcgcggcgca tttacttggg tcacgcgcaa actgaaaggc cacgtttcgc
     4621 tgccgctggt gaccaccaac cggattaacg atccgcaggt tgccgacgac attctctcgc
     4681 gcggcgatgc cgatatggta tcgatggcac gaccgtttct tgctgatgcg gaactgctgt
     4741 caaaagcgca atcggggcga gccgatgaga tcaacacctg cattggctgc aatcaggcct
     4801 gcctcgatca gatcttcgtt ggcaaagtca cctcgtgcct ggtgaatcct cgcgcctgcc
     4861 acgaaaccaa aatgccaatc cttcctgccg tgcagaaaaa aaatctggcg gtggtcggtg
     4921 cgggacctgc tgggctggcg tttgccatta acgcggcggc gcgtgggcat catgtaacat
     4981 tgtttgatgc ccacagcgag atcggcgggc agtttaatat cgccaaacag atccccggca
     5041 aagaggagtt ttacgaaacg ctgcgctatt accaccggat gatcgaagtg acgggcgtga
     5101 cgctgaaact caatcacacc gtgacggcgg atcagttaca ggcgttcgat gaaacgatcc
     5161 tcgccagtgg gatcgtgccg cgcgttccgc ctatcgacgg gatcgatcat ccgaaggtat
     5221 tgagttatct cgatgtactg cgcgacaaag cgccggtagg caacaaggtt gccatcatcg
     5281 gttgtggcgg gattggtttt gatacggcga tgtatttaag tcagccgggc gaatccacca
     5341 gccagaatat cgccaggttc tgtaatgagt gggggatcga cagcagccta caacaggctg
     5401 gtggcttaag cccgcaggga atgcagatcc cgcgtagccc acggcaaatt gtgatgctcc
     5461 agcgcaaagc cagcaaaccg ggacaggggt taggcaaaac caccggctgg atccatcgca
     5521 ccaccctgct ctcgcggggt gtgaaaatga tcccaggcgt aagttatcag aagattgacg
     5581 atgacgggct gcatgtggtg atcaacggcg aaacgcaggt attagcagtg gacaatgtgg
     5641 tgatctgcgc agggcaagag ccaaaccgcg cgctggcgca accgctgatt gatagcggga
     5701 aaactgtgca tttaattggc ggctgcgatg tggctatgga gctggacgca cgacgggcaa
     5761 ttgcccaggg aacacggctg gcgctggaga
//
LOCUS       DAZGLJ010000266         5695 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000266 DAZGLJ010000000
VERSION     DAZGLJ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.266"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            226..1158
                     /gene="rfaD"
                     /locus_tag="ACHK49_004426"
     CDS             226..1158
                     /gene="rfaD"
                     /locus_tag="ACHK49_004426"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO9264941.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1168..2214
                     /gene="rfaF"
                     /locus_tag="ACHK49_004427"
     CDS             1168..2214
                     /gene="rfaF"
                     /locus_tag="ACHK49_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO9264942.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2218..3210
                     /gene="rfaC"
                     /locus_tag="ACHK49_004428"
     CDS             2218..3210
                     /gene="rfaC"
                     /locus_tag="ACHK49_004428"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO9264943.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3207..4415
                     /gene="rfaL"
                     /locus_tag="ACHK49_004429"
     CDS             3207..4415
                     /gene="rfaL"
                     /locus_tag="ACHK49_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO9264944.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNSVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(4451..5593)
                     /locus_tag="ACHK49_004430"
     CDS             complement(4451..5593)
                     /locus_tag="ACHK49_004430"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFO9264945.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
     gene            complement(5602..>5695)
                     /locus_tag="ACHK49_004431"
     CDS             complement(5602..>5695)
                     /locus_tag="ACHK49_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFO9264946.1"
                     /translation="EYKEKYKHLLYQKKFLDGVFTAIKYNVMKG"
BASE COUNT         1575 a         1248 c         1273 g         1599 t
ORIGIN      
        1 aatgcttccg ttatttatca tacatcggaa tattgatact aaagcactat tcacatgcaa
       61 aaccaacatc cgccatgaag gactagctaa aacccaaact agtttgttgc aattagcatc
      121 cttgcacctc tatgtaaagg gctgaaggga ttcggatgtg atggtatgat tacagacatt
      181 cgtgtctgag attgtctctg actccataat tcgaaggtta cagttatgat catcgttacc
      241 ggcggcgcgg gctttatcgg cagcaacatc gttaaagccc tgaatgataa aggcatcacc
      301 gatattctgg tggtggacaa cctgaaagac ggcaccaagt ttgtgaacct ggtggatctg
      361 aatatcgcag actatatgga taaggaagac ttcctgatcc agattatggc tggcgaagag
      421 ttcggcgatg tcgaagcgat tttccacgaa ggcgcgtgct cttccaccac cgagtgggac
      481 ggcaagtata tgatggataa caactatcaa tactccaagg agctgctgca ctactgcctg
      541 gagcgtgaaa tcccgttcct gtacgcttct tccgcagcca cctacggcgg acgcacctcc
      601 gactttattg aatcccgcga gtacgaaaaa ccgttgaacg tctacggtta ctcaaaattc
      661 ctgtttgatg aatatgttcg ccaaatcctg ccggaagcga actcgcagat tgttggcttc
      721 cgctatttca acgtttatgg accgcgtgaa ggccataaag gcagcatggc gagcgtcgct
      781 ttccatctca acacccagct taataacggt gaatcaccga agctgttcga aggtagcgag
      841 aactttaaac gcgacttcgt ctatgttggc gatgtggcag atgtaaacct gtggttcctg
      901 gaaaatggcg tttccggcat cttcaacctc ggcaccggtc gtgcggaatc cttccaggct
      961 gtagctgatg ctacgctggc ttatcacaag aaaggccaga tcgaatacat tccgttcccg
     1021 gataagctga aaggccgcta ccaggcgttc actcaggcag atctgacaaa tctgcgcgcg
     1081 gcgggttacg acaaaccgtt caaaaccgtt gctgaaggtg taacggaata catggcctgg
     1141 ctgaatcgcg acgcataaga gctctgcatg aaaatactgg tgatcggccc gtcttgggtt
     1201 ggcgacatga tgatgtcgca aagtctctat cgcacgctcc aggcgcgcta tccccaggcg
     1261 ataatcgatg tgatggcacc ggcatggtgc cgtccattat tatcgcggat gccggaagtt
     1321 aacgaagcta tccctatgcc tctcggtcac ggagcgctgg aaatcggcga acgccgcaaa
     1381 ctgggtcata gcctgcgtga aaagcgctac gaccgcgcct acgtcttacc taactccttc
     1441 aaatccgcat tagtgccttt cttcgcgggt attcctcatc gcaccggctg gcgcggcgag
     1501 atgcgctacg gtttactcaa cgatgtacgc gtgctcgata aagaagcctg gccgctaatg
     1561 gtggaacgct atatagcgct ggcctatgac aaaggcatta tgcgcacagc acaagatctg
     1621 ccgcagccat tgttatggcc gcagttgcag gtgagcgaag gtgaaaaatc atatacctgt
     1681 aatcaatttt cgctttcatc agaacgtccg atgattggtt tttgcccggg tgcggagttt
     1741 ggtccggcaa aacgctggcc acactaccac tatgcggagc tggcaaagca gctgattgat
     1801 gaaggttatc aggtggttct gtttggctcg gcgaaagatc atgaagcagg caatgagatt
     1861 cttgccgctt taaataccga gcagcaggca tggtgtcgga acctggcggg ggaaacacag
     1921 cttgatcaag cggttatcct gattgcagcc tgtaaagcca ttgtcactaa cgattctggc
     1981 ctgatgcacg ttgcggcggc gctcaatcgt ccgctggttg ccctgtatgg tccaagtagc
     2041 ccggacttca caccgccgct atcccataaa gcgcgcgtga tccgtctgat taccggctat
     2101 cacaaagtgc gtaaaggtga cgctgcggag ggttatcacc agagcttgat cgacattact
     2161 ccccagcgcg tactggaaga actcaacgcg ctattgttac aagaggaagc ctgacggatg
     2221 cgggttttga tcgttaaaac atcgtcgatg ggcgatgttc tccatacgtt gcccgcactc
     2281 actgatgccc agcaggcaat cccagggatt aagtttgact gggtggtgga agaagggttc
     2341 gcacagattc cttcctggca cgctgccgtt gagcgagtta ttcctgtggc aatacgtcgc
     2401 tggcgtaaag cctggttctc ggcccccata aaagctgaac gcaaagcgtt tcgtgaagcg
     2461 ctacaagcag agaactatga cgcagttatc gacgctcagg ggctggtaaa aagcgcggca
     2521 ctggtgacac gtctggcgca tggcgtaaag catggattgg actggcaaac cgctcgcgaa
     2581 cctttagcca gcctgtttta caattgtaag catcatattg caaaacagca gcacgccgta
     2641 gaacgcaccc gcgaactgtt tgccaaaagt ttgggctata gcaaaccgca aacccagggc
     2701 gattatgcta tcgcacagca ttttctgacg aacctgccta cagatgctgg cgaatatgcc
     2761 gtatttcttc atgcgacgac ccgtgatgat aaacactggc cggaagaaca ctggcgagaa
     2821 ttgattggtt tactggctga ttcaggaata cggattaaac ttccgtgggg cgcgccgcat
     2881 gaggaagaac gggcgaaacg actggcggaa ggatttgctt atgttgaagt attgccgaag
     2941 atgagtctgg aaggcgttgc ccgcgtgctg gccggggcta aatttgtagt gtcggtggat
     3001 acggggttaa gccatttaac ggcggcactg gatagaccca atatcacggt ttatggacca
     3061 accgatccgg gattaattgg tgggtatggg aagaatcaag tagagtgtcg ttctacaagt
     3121 atgtctcttg cagatttgcc agctcaaacc gtttttcaaa atttaaatct tgaaataata
     3181 accaataagt tgacatcgga gataagatga cctcaacatt atttttctct ctcgagaaaa
     3241 aaaactggat agcgtactgg aacagagctc tcgtattctt attcattacc acctattttt
     3301 tgggtgggat tacaaggtat aaacatctta ttgttattct tatgacaata acgacaatcg
     3361 tctatctctg caaacggcca aaacactatc tctcactatt taaaacattt ctttttggta
     3421 gtgttgccat attaactatt gctgcattgc tgtcacttct tcaatcccct gatgcaggtg
     3481 ctagcatgaa ggaaattttc aaagctatta ttgagaatac tttactatgc acaatagcaa
     3541 taccgatcat attgagagac gagaaaagag aagatgtcga aaaaatcgtt ttcttctcat
     3601 ttattagtgc gttgggctta cgctgttttt ctgaattgat tacctattat aaggactatc
     3661 aacaagggat aatgccattc gcagattata gacaccgtag catttctgac tcgatggtct
     3721 ttttattccc tgcattgtta aatctctggc ttatcaaatc agcaaaatac cgcatttctt
     3781 ttgtggttct aagcgttatt tttatttttc tgatattagg aactttatcc agaggggcct
     3841 ggctttccgt gttagtcatt ggattaatat ggattctgat gtttaaacaa tggaagttac
     3901 tattagtagg agtaatggtt gccatcattg cattgtcggt tattttcaca cataaggaga
     3961 tgaccgcaaa gctaacgtat aaacttcaac aaactaatag ttcttatcgc tatgcaaatg
     4021 gtactcaagg cagcgcactc gatctaatat tagaaaatcc tgttattggt tatggttacg
     4081 gtaacgttgc atataaagat gtctataata aacgtgtcat tgattatcca gaatggacct
     4141 ttagacaatc aatagggcca cataattttg cgctattcat ctggtttggc actggtttat
     4201 tagggctggt aagtcttatg atgctatact gtgcaatatt gaaagagtgt ataaaaaata
     4261 gcgtcaagaa taaatatcgc tcaccatata atgcatatta tataatctta ctatctttta
     4321 taggttattt tgttatccgt ggaaacgtag aacaaattga accaaattta ttaggcgttt
     4381 acgccggctt attattagcg atgaaaaaca agtaaaaaat aaaaaaggct gcataatgcg
     4441 gcctttttat ttattcaaac caattatgaa taacctcttc gaatctttga gttacgccat
     4501 cccaactgta gtgttcaaac acaaagtcct gaccttgctt tgcaactgct gttaattcag
     4561 gattcgctaa cgtttttaaa atatcgctgc ttatggagtc tgcagtcata ggttctttta
     4621 aatggaatcc cgtagtattt tctttgacaa attccgtcat ccccccacgc gtactcacta
     4681 aaacaggctt accagcaccc atagcttcaa ttgccaccat gcaaaatggt tcctggaact
     4741 gggaaggaat gacgactaaa tctgccagtg ggtaataaca atgcatcttt tcaggcggta
     4801 tgcttcctaa cataatacaa cgatccttca aacgttttgc aatttccctg acattgcgtt
     4861 gatatgcacc tttgtcactt ttactcattt ctgtataatc accaatcaca actaatttta
     4921 aatttttgtg agcagcggcc aatttttcga atgattgcat caacagcaat atgcctttgt
     4981 cagggacgat acgcccagca taaaaaataa ttttctcttc aggggagata ttaagttcgc
     5041 ttctagtgat cggctgaaaa ttagattgat aggtttctaa atcaattcca tttggtacaa
     5101 tctcaatatc tgcatttgct aaataagatt ggtaatactt tttcaagtat aaactaggca
     5161 ctatcatttt aacattttgc tcaagcaatt ttggttcaaa tgcattatgc atatgtataa
     5221 ccactcttgc ctgaggagct cgcttacgaa tttgagtgta tagcttcatg ctattatgaa
     5281 ctacaataac actttctttc gttataggaa aatcatgtgc aatattcaat attctctgtg
     5341 aatatggaag aggatcaagt cttgtccatt tttgaaataa ccgcttatat atgcgactga
     5401 atccaaccct atgaatacta caattatcat taacaaaggt gtaatttgag tatcctggat
     5461 ttttgataca aacaatacga tttggaaaat tggtacgctg agcaacctga tacatccacg
     5521 tttcaacagc agccgctccg cgaggaggaa ttgaaaaaat aggagtaacc gtaaatatta
     5581 ttttatcaac catctcgtat attaaccttt cataacatta tatttaattg ctgtaaaaac
     5641 accatcgaga aattttttct ggtaaagcaa gtgtttatat ttttctttgt actca
//
LOCUS       DAZGLJ010000267         5677 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000267 DAZGLJ010000000
VERSION     DAZGLJ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.267"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(135..211)
                     /locus_tag="ACHK49_004432"
     tRNA            complement(135..211)
                     /locus_tag="ACHK49_004432"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(175..177),aa:Arg,seq:tct)
     gene            complement(225..301)
                     /locus_tag="ACHK49_004433"
     tRNA            complement(225..301)
                     /locus_tag="ACHK49_004433"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(265..267),aa:Arg,seq:tcg)
     gene            complement(311..386)
                     /locus_tag="ACHK49_004434"
     tRNA            complement(311..386)
                     /locus_tag="ACHK49_004434"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(351..353),aa:Ile,seq:cat)
     gene            complement(415..1128)
                     /locus_tag="ACHK49_004435"
     CDS             complement(415..1128)
                     /locus_tag="ACHK49_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9264947.1"
                     /translation="MAVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRELFLMH
                     YGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGE
                     ATDMFLMMACLSLFETDERMSLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHM
                     SESLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRHGYGQAIAEKCGYSSRSYFISVF
                     HRYYGFPPDRYVSRQGLDY"
     gene            complement(1263..1460)
                     /locus_tag="ACHK49_004436"
     CDS             complement(1263..1460)
                     /locus_tag="ACHK49_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO9264948.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEVAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            complement(1702..2232)
                     /locus_tag="ACHK49_004437"
     CDS             complement(1702..2232)
                     /locus_tag="ACHK49_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO9264949.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            complement(2241..2600)
                     /locus_tag="ACHK49_004438"
     CDS             complement(2241..2600)
                     /locus_tag="ACHK49_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9264950.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            complement(2613..3659)
                     /locus_tag="ACHK49_004439"
     CDS             complement(2613..3659)
                     /locus_tag="ACHK49_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9264951.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAQQNLTAWMIDVIRHAISGAQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQKPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTHWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
     gene            complement(3661..3933)
                     /locus_tag="ACHK49_004440"
     CDS             complement(3661..3933)
                     /locus_tag="ACHK49_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264952.1"
                     /translation="MAHNTKHHNSDIAPASRQGRCSHTFKYDWYQHDPCTEEQAEWLI
                     HNYRRRGYEFKKALSLDYRHWIIYVRLLYSERPPRPSRTFQQRIWR"
     gene            4069..4326
                     /locus_tag="ACHK49_004441"
     CDS             4069..4326
                     /locus_tag="ACHK49_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO9264953.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRSGK"
     gene            4332..4631
                     /locus_tag="ACHK49_004442"
     CDS             4332..4631
                     /locus_tag="ACHK49_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9264954.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGNVIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDSRKVFIVGIYHGGQSYETDFL"
     gene            complement(4836..5180)
                     /locus_tag="ACHK49_004443"
     CDS             complement(4836..5180)
                     /locus_tag="ACHK49_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264955.1"
                     /translation="MTTFTREQLIAHAEETIETQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            complement(5177..5542)
                     /locus_tag="ACHK49_004444"
     CDS             complement(5177..5542)
                     /locus_tag="ACHK49_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO9264956.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            complement(5544..>5677)
                     /locus_tag="ACHK49_004445"
     CDS             complement(5544..>5677)
                     /locus_tag="ACHK49_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO9264957.1"
                     /translation="PIPPLLLVWFIVKIIEPVIELYNDVVWASFSTLHNKINPYKES"
BASE COUNT         1428 a         1517 c         1265 g         1467 t
ORIGIN      
        1 ggagcgatct gcccacagag aaagtcacac tgaccactcc gtaagctcac ccccgaaagg
       61 ctctgtggtt gatatgcgcc gggcgtggcg cagatacaaa aaaggcccgc cgaagcgagc
      121 ctggaaaata agtgtggcgc gttgtagtgg agtctaacca ctgaccgatt gcttagaagg
      181 caattgctct gtccgactga gctaacaacg catgatgctg ataatggacc gccatcgggg
      241 acttgacccc cgcgcagcca gcttcgaagg ctggcgctct gtcccgatga gctaatagcg
      301 gtatgtaata tggtggccct tgctggattt gaaccagcga cctggcgatt atgagtcgct
      361 cgctctcacc actgagctaa agggccgagc caaaaaataa taatcagatg aaaatcaata
      421 atcaagccct tgcctggata catatctgtc tggcgggaag ccataatagc ggtgaaatac
      481 agaaataaag taggacctgc ttgaataacc gcatttttct gctatagcct gtccatatcc
      541 atgccgggaa cataacatat ttacagcaac acgcatccgc tcttccagca acaagcgact
      601 gaacatgccc ccttcatttt tcagttttgt ctttaacaaa ctctcactca tatgcaactg
      661 tagagcaatc gcaccaagcg tccagcttgc tgatatatct gtctgaatta tcgccctgac
      721 tttggcactt atgctggata aacatccact taaaaacaat gacatccgtt catctgtttc
      781 aaacagagac aggcaggcca tcataagaaa catatccgtg gcctctccgg aaaatccctg
      841 gctggtaatt aaagccgcag ccaacgcagg attgttgggt tccagcaaca ggtaaagcgg
      901 aatgtcagtc agacgagtcc tcgtcagctt atgctgactt tccagatatt gacttacgac
      961 ggattcgctt atatcgacaa ttttaacttt gccataatgc ataaggaaaa gctccctgat
     1021 gcatttggtg gccagaacga ctgagcctgg cttaagtgac aacgtatcct tttcaagaaa
     1081 aatattaatt ggggagcaaa ccatgataac tgaacagaca acagccatta taattttact
     1141 ttcattagca attggttagc tcaattatag ccccaaaagg taaattatca tcaacacata
     1201 agcaaaggac tgacaggtgt cgccccccca ccagccgccc attcaccaca aataaaaagc
     1261 cttcaggact gaaggcgtct gtaacaaccg cactgatagt ctgccagacc cgccataaca
     1321 agctgggtca gtattaactg gcagcgttcg cgtgaaaggt aagtattctg cgcaacctcc
     1381 ccgactgtcg ccggttcggt aacgcttaat tcattaaaca ccactctggc ggtttcggtc
     1441 atatcctgct gtttcagcat gtctttttcc ctttttcggt taacgtgaca caccaataac
     1501 tcttgtcgaa aaagccagca agctgaaaga acggtattaa taaccaccag cgaatttatt
     1561 gcgctgctgt atattacgga cacaaaaaaa ccaccttccg gtggcttcct tgtgcgaaaa
     1621 aacttgcatt tcgcctcgcg atacagcttt gcgaagctta caggaattca agctgtttct
     1681 gcgtaaaaaa gcaagctttt tttatcgaaa tgaatcgtgc ataggtacat aaagcatgtg
     1741 ttcagccacg gctaaccaac ctgcaatacg tttctcacat gtgctgaaac accattccgg
     1801 gtgagtacga tttaaacatt ctgccatttt tctcttactc attccccgtc cttcgtacct
     1861 ttgccggaga atattgatta gcccgggata ttccccaagc acctcactga taacgcgatc
     1921 aataatcaac gcctctgtgt ctgtacaatg tgacaaccag ctcttctgct tccctctggt
     1981 catatcccga aaaaatgcct caagttccgg tttttccagc ccggatttct tcatgctgcg
     2041 taaaacctca ttaactgctg ttttcgtcag ctttttcgaa accagtaacc ggttaaacat
     2101 atttccggat ttacccccac cgatatacga ccaccgcccc cacatccgta atttcccctg
     2161 gatccagact gcttccagcg tgttcaggcg taaatgttcg ccgcttttgc ctgtaatttc
     2221 cggatatatc atatttacgc tcactcactc tcaattttgt aaatcttcac acccagccgt
     2281 ccaccagata ctggctgacc gcgcacaata ttgatttcat caaactgctc gtcatcaatg
     2341 agcacttccg catgcgtcag cgcatccagc ggtgctttca gaatgttgtc caggtcacgg
     2401 cggcgcttat ccggtggctc tgcaatcacc tttatcgcca gccttccgga caggcttaat
     2461 ttcagccgct gctggcgaac aataagcgcc acagcccggc gataacgctt tccctcctcc
     2521 gagataaaat atgtgctgcc acggcgtcgc cagtaagtgt tcaccgtcgg cgggtaaggt
     2581 aaaaccaaat ctatgagcat cagtcacctc ttttacccaa gcacgccagt tgcaaaggcg
     2641 tgatcaagaa aacgaaaaat taaatcaacc tgagaaccat gcttttcttc gaacgccagc
     2701 ggatccgcat gaagctcgtt gtgatgctcc cgacacagcg gtagcgtgaa aatatcgtga
     2761 gattttgtcc ccattccccc ctgaccatga ccaatcaggt gatgcggatc gtcagcaggc
     2821 ttaccacaac acgcacacgg ctgtgtcttc acccagtgcg tgtatttctc gttaacccag
     2881 cggcgacgtt taggtcgttt catgaaagat tccggagact caggatcaac ggcaatgctg
     2941 accaccgtct tttcctgtgg cggtttttgc tggtgagcgt gaggcagcgg cgcaagattt
     3001 tttgtgcgct gcttcagtat gctggtggcg gtctgctctc ccggtacgat gtcgctttca
     3061 cggtacattg agcggatttt ttccgcacgc aaccccagcg aacgacgtaa taccgcttcc
     3121 ggtagcgcgt ccgccacctg attgcggacc gcccaccagg ataattcagc cagagataat
     3181 tcccgttcct gtgcaccact tattgcgtga cgtatgacgt caatcatcca ggctgtcaga
     3241 ttttgttgag caagctgccc gagtgattcg gatgtctggt cgcgcagctg gttgtcgcag
     3301 tgccagcaca acaccattgc gccggtacca taacggtgaa tgacggtttc gctgtgatga
     3361 taatcgccgt gtggccactg gcaggattta atatggcgca acagccagtc agacaatgca
     3421 ccagcaccac cagcagcacg aatcacccgt gcgttactga aaaacggcag caatgttttg
     3481 tcttccacta gcggctggcg aacggcagga acaaccccgg acggcagatt acgcatgctt
     3541 ttcggttccg gctccaccag tacccgggta ttgtggaata ccggcatgga ttcacgaccc
     3601 ggcttaacga tcaccagccc gagttccggt accagaacag gtcgaagtaa tacccgcacg
     3661 ttacctccag atgcgttgct ggaatgtgcg ggacggacgc ggcgggcgtt cggaataaag
     3721 gagcctgacg tagattatcc agtgacggta gtcgaggcta agggctttct taaactcata
     3781 cccacgtctg cggtagttat gaatcagcca ttcggcctgt tcttcagtgc agggatcgtg
     3841 ctgataccag tcatatttga atgtgtgaga acaccgccct tgcctgctgg caggggcgat
     3901 atcagaattg tgatgtttgg tattgtgcgc catcggtttt ctctgctggc gcagcaggtg
     3961 ccagttgttc aagctggcgt gcggcaatat tgtctctgat ttctgttgtc gtcaacaggc
     4021 aacgtgctat catcgaatgg tgttctatcc tactccgtga ggtttaccat gcgtacaacc
     4081 caacaattca gcattacatt aactaacgaa atggctgaca tggtgcgcgc ccgtgtggct
     4141 tccggtgcct atgcttcaga aagcgaggtc attcgtgaag ggcttcgcgc actgaatgag
     4201 cgcgataaag caatcgaagc gtggttaacg cattcagccg ccccctctct tgattctatc
     4261 cgcgaaaatc caaacaacgg acgctccatt tcacaggttc gcgccgcgat tcgatccggg
     4321 aagtaatctg catgacatat gaagtcatca ttactcctga ggccgaacaa caaataatca
     4381 acctgcacag gtatataacg gagaaagcag ggaacgtcat tgctgacaat tatgccaatg
     4441 cgcttcttga ttatcttgat gggttttcta cattcccgca tcggggcaat aaacgcgatg
     4501 atattcgcca ggggatgcgg gtaactcatt tccgccacag aacgattatt gcttttgccg
     4561 ttgatagcag aaaagtcttt attgtcggta tctatcatgg tgggcaaagt tatgaaaccg
     4621 atttcttata aacttttacc cacatcattc cggtgttaga attaaccgtc cgccccctct
     4681 cttactggcg gattcgtagg ctatataaat caaagatccc ggctcatgtt tatgtcggga
     4741 tcttttttcg gcgatttatc cccagcggca aatcgaatac accaccagcg ccaccgccat
     4801 cgcaattcct accgttgtga atgcttcagg ccaggtcatc gtaaaatatc ctccacgctt
     4861 atcagtccgt tccgctccag ataactcatc gccttatccg gtaatttgca gtctggcttc
     4921 gctttcctca gttgccaggt taactgcttt accagcatgg ttaactcatc gaccagacgc
     4981 tgatatccca ctggtttgta ttcatgcaat ttaccggctg gctctgctgc cagcgatacc
     5041 agtgcgattt ccagaacagc aatatccatc ttatatgtgc ggatgatgtc atggtcgatt
     5101 gtgcccggta tgcacagtct ctgtgtttca atagtctcct ctgcgtgagc tattaactgc
     5161 tctctggtaa aagtcgtcat gccgtagccc cttcttgata tttttcaaac cagaacacaa
     5221 ctgggtcaga tttcatttca accaatccca tacgaaccag cgctttgcct ttcccggacg
     5281 caaggaattc acgacgacca tcactgataa ttcgccgata atcttccaga ctactgcaat
     5341 gcttgtgcag attgcatggg tggcatgccg gaaccatgtt ggatatatcg tcacgttcct
     5401 ggtgaagcat atttccatca aaacgaatga ccggttttac atggtctgca tgccactttt
     5461 cgccaagttc gcagccgcaa taagcacagc gaccgccgaa cttcatgcgc agctctgcac
     5521 gttgtttttt cgtcagtgcc atatcagctt tccttatacg gattaatttt attgtgcagt
     5581 gtgctgaacg acgcccacac cacgtcgtta tacaattcaa taactggctc aattattttc
     5641 acgataaacc agaccagtaa tagcgggggt atcggta
//
LOCUS       DAZGLJ010000268         5665 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000268 DAZGLJ010000000
VERSION     DAZGLJ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.268"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..299)
                     /locus_tag="ACHK49_004446"
     CDS             complement(<1..299)
                     /locus_tag="ACHK49_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFO9264958.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGL"
     gene            complement(296..1966)
                     /gene="menD"
                     /locus_tag="ACHK49_004447"
     CDS             complement(296..1966)
                     /gene="menD"
                     /locus_tag="ACHK49_004447"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO9264959.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(2055..3350)
                     /gene="menF"
                     /locus_tag="ACHK49_004448"
     CDS             complement(2055..3350)
                     /gene="menF"
                     /locus_tag="ACHK49_004448"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO9264960.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3429..3734)
                     /gene="elaB"
                     /locus_tag="ACHK49_004449"
     CDS             complement(3429..3734)
                     /gene="elaB"
                     /locus_tag="ACHK49_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO9264961.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(3789..4250)
                     /locus_tag="ACHK49_004450"
     CDS             complement(3789..4250)
                     /locus_tag="ACHK49_004450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9264962.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            4315..5232
                     /gene="rbn"
                     /locus_tag="ACHK49_004451"
     CDS             4315..5232
                     /gene="rbn"
                     /locus_tag="ACHK49_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO9264963.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            5420..>5665
                     /locus_tag="ACHK49_004452"
     CDS             5420..>5665
                     /locus_tag="ACHK49_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFO9264964.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSI"
BASE COUNT         1357 a         1555 c         1394 g         1359 t
ORIGIN      
        1 agcccctggc aagccgccat catcgccacc cgtccgccaa gcgagtaccc caccagccag
       61 aagtcaagga tgttgtaact aaccaaggtt ttacgcagta agtcggtgac atcatcaaat
      121 ccatcgacgc taatcgccgc cgaaccacca tgacctggga gatcaacata caaccgtgag
      181 tagtcggcaa acgcctcgcc cacttcttgc cattcgtggc aatcgccgga aaaaccgtgc
      241 aaaaacacca gccagggtaa acctggtttt ccgtgttttg cctgcgcgtg caggatcata
      301 aatggcttac ctgcgccaga agttgctgga gcgtttgcgc accatcggtg tcgttaacca
      361 ccatttcaat caccgtggtg gttggcgtgc gccaggcgtc ggcaaatgcc gtttcaagtt
      421 cctgccagtt ttgcggacga tgatatttca gctcgaacat cgcggcggcg tgctcaaaat
      481 ggacgttttg cggcatcaga tagaaacgct cacgctcgct ttgcggcgtt ggcaacagcg
      541 agaaaatttg cccgccgttg ttgttcacca caattaatac cagcggcgca gaaacctgac
      601 gcaataacgc cagcgcgttg agatcgtaaa gtgcggagag atcgcccaca atcgccagcg
      661 tcggtttgcc gcttgcccgc tgaacgcctg cggcggtcga aagcagtccg tcgataccgc
      721 tggcccctcg gttgctgtac accgggtaac ctgccggaag ttgcgaaagc gcatcaatca
      781 gacgtaccac caggctgtta ccaacaaaca attgcccctg ttcaggcaga tagtcgctga
      841 tgcgatgcgc cagttgcgct tcgccaaacg catcacggcg ggcaataacc gcctgcattg
      901 cctgttccgc caggcgcggg atttcaacgc accagggctg gcgtttttct gccggatgca
      961 gctccagcca gtcggcaata ttggcaatta agcgacgtcc gcgatggtgt gccggatcaa
     1021 gtcgcccttc aatgtcatca acaatccagt actcttcggg ttcacagctt gcctgccatt
     1081 gcaggagccg tttccccgtc aggctgcttc ccagttgcac cacaatttgc gcctgctgca
     1141 gctcgctggt cgctttggca ttgcctaacc aaagatcggc acacggcagc ggctgcccgg
     1201 tttgtgacag cacatcgcca atcagcggcc agccaagagt ttgcgcccac agggcaactt
     1261 ttttgccctc ttccgcactc atgcgcccgg caaccaccac gccgcgcttt tgtcgccaga
     1321 agaaccagtc gcgctgtttt tcactttcca gacgaggcgc ttcacgcagc cacggtttgt
     1381 cgtcctgcca ccagtcaccc agacgctgtt gccagctaag cccggtatcg tccatttcgc
     1441 catacagcgg ttcagcaaac gggcagttga tatggactcc ccccgcatga agcgtaccga
     1501 gagcgtggtc gatggtagaa accagccaac gtgcggggat atcctgggtc gggcgcggca
     1561 atgaaatact gtgcgtgggg tgagaggcga acattcccgg ctggcgaatt gcctgattcg
     1621 cgccgcagtc aattagctcc ggcgggcgat cggcggttaa gagaatcagt ttttctccgg
     1681 ttaacccggc ttcaatcagt gccggataga gatttgccac cgccgtgccg gaggtcacaa
     1741 tcaccgccac cggctgcttg ctgactttcg ccagccccag cgccagatgc cccagtccac
     1801 gctcatcgaa atgggtgtgg tgaatgaatg cggaattctc cgccgccgct aacgttaacg
     1861 gtgtagaacg cgagcctggg gcgatacaga tgtgtctgac gccgtgacgc gttaatgctt
     1921 ccagaatgac cgccgcccag cgtcggttaa atgcgcttac tgacatgagt ttgtccggta
     1981 tcaatattgc ggctaagtat aaggagctac aaaaatagat tattgatatg aatcggtaat
     2041 gatgcgactc attactattc catttgtaat aaagtacgca gccctgccgc tttgttgtcg
     2101 atttcctgcc actcttgctc ggggtcggaa ccacggacaa tgcccgcgcc agcatatagc
     2161 cgcacgacat tgccgctgat ttttgctgaa cgcagggaaa cgcagaattc gctttgttgt
     2221 aatgagagat agcccgcaga accggcgtac cattctcggg tgaacggttc gtgacgggcg
     2281 ataaactgtc gcgccagatc gcgcggtaag ccagcaactg ctgccgttgg ctgcaactga
     2341 tgtaaacaga tcacatcatc cgctttgttg agtgaagtcc agatacagcg gcgaagatgc
     2401 tgcactttac gcagacgcag tacctgcggc ggtaaaacat ccagcgtctg ggtatcggcc
     2461 tgtaatcgtt ggcagatatc ttccaccacc agcatgttct cgcgctggtt tttatcatcc
     2521 gccatcagcc actctcctaa ctgctgcgcc tgcttatcat caggattatt tgctactgtt
     2581 cccgccagcg cttcagtacg cagcgctttg tcacgccgcc gccataaccg ttccggtgaa
     2641 gagccaagaa aagcattttc gccatcaaag gccatgtaaa aatggtagca attcagattc
     2701 agtcgacgac tggcagccat catcgccgcc gcgttgaccg gacttgcgaa atgcaggtca
     2761 gttgcccgag cgagcaccac tttgtcgagc tcaccttcgg cgatggtttt cgttgccagt
     2821 tcgattaatt gcgtccagcc cgttttgtcc ggccagtgtt gttctcgcgt ggtggttaaa
     2881 tgtaacccag gcaagggctt gatactcacc agtgtggcga taaattcttt tgcctgaatc
     2941 gcatcgtgct gaagggagct ttcgctgaat agcgtcagcc gcagcgtggc tttaccgcca
     3001 cagcgtcgcc attccaggcg gggtaaaagt aaattgccct gcgacgggtc aaaagcattc
     3061 agcccccaaa tgcgtaagtc ggcgtgttcc gggtgctggc gaagaaaacg ttgtgcctgg
     3121 tccaacgacg taaaacgggt aatcgcgccc aggacggctg cttcttcatc accattacgt
     3181 tgttgccagt agaattgcgg gtacgtttgc tgactggcca gccagctcaa ggcatcaaaa
     3241 gcgtctttga gagggaaagg aatatcgata acccgaatgc cgggtgtcgc cggaatctct
     3301 tgcgacaaat ggcgcagtag attttccagc gccgtagtaa gtgattgcac gcggacctct
     3361 ccctgttaaa agcctcacat tatacggggt actacaaaaa aaatgcagta ccccggtgta
     3421 gggaggtttt aacggcgtgc cagcaacagt cctagtacca gcccaacggc cgcgcccaca
     3481 ccaattcctt gccagggttt ttcgtggacg tagtcatcag cacgataaac tgcctgcttc
     3541 gcccgatagt aataactgtc tgaagcctgg ctaacccgtt ttttcacatc atccagcgct
     3601 ttttctgcac gcgctttcag ctcaacatat ttctgatcgg cgggatcgcc agaggagcgg
     3661 agcacctctt ccagtgtttc actaagcagc gtcaggtcgt catcgatacg tgtatcacca
     3721 aactgattag acattctcgt tctccatttg cgtaaaacct gtcactaact atagacaagg
     3781 gttgttgatt acgcctgaat tacctcgcgt gccatcccaa tgtgtgggat accatcttct
     3841 tcatagacct ccgtcacagg gataaaaccg aaactctggt agaagttttg cagatgcgcc
     3901 tgcgccccga ggtaaacagg cttatcaggc cagtgatgcg tacatgtttc gagtgtttta
     3961 ctcatcagct gctgccccac tttttcgcca cgcaacgctt cactgacaat cacccgacct
     4021 ataacgaccg gctcaagatc atcatcactt ttcagaatcc tcgcatatgc caccagttca
     4081 tcatttttcc aaccgagaat atggcgatta tcccccgtca aatcatcacc atcgatatcc
     4141 tgataagggc aattctgctc gacaacaaac accgcgcaac gcagttgtaa taaggcataa
     4201 agctgggaaa ccgataattc agagtgatgc agatcttgcc attcaatcat aatgctctcc
     4261 ttttagatgt atgccttata ctagccggaa tattttttga aacgtgatga actcatggaa
     4321 ttaatttttt taggtacttc ggccggtgtg ccaacccgca cgcgcaatgt cacggcaata
     4381 ttgcttaacc tgcaacatcc gacccagagc ggtctttggt tgtttgactg cggtgaaggc
     4441 acccagcatc agctactgca taccgccttt aaccctggaa aactggacaa gatttttatc
     4501 agtcaccttc atggcgatca tctttttggt ttacccggct tgctgtgcag tcgttctatg
     4561 tcaggcatta tccaaccctt aacgatttat ggtccgcaag gtatccgtga atttgtggaa
     4621 accgcgctgc ggattagcgg ctcatggacc gattatccgc tggaaattgt cgaaattggc
     4681 gctggcgaaa ttctcgatga tggcctgcgc aaagtaaccg cttatccgct ggaacaccca
     4741 ctggaatgtt atggctatcg tattgaagaa catgataaac cgggtgcatt aaatgcccag
     4801 gcattaaaag ctgctggcgt gccgcctggc ccactgtttc aggaattaaa agcgggcaaa
     4861 acaatcacgc tggaagatgg aaggcagatt aacggcgcag attacttagc tgctccagtg
     4921 ccaggtaaag cgctcgctat tttcggcgat accggcccct gcgatgccgc acttgacctg
     4981 gctaaaggtg tcgatgtcat ggtgcacgaa gcgacgctgg atataaccat ggaagccaaa
     5041 gccaatagtc gcggccatag ctctacacgc caggctgcga cactagcccg tgaggctgga
     5101 gtcggtaagc taatcattac ccacgtcagc tcgcgctatg atgacaaagg ttgtcagcac
     5161 ctgttacgtg aatgcaggtc aattttcccg gcgactgaac tagcgaatga tttcaccgtg
     5221 tttaacgttt aactctgctt attattaaaa acagggcgaa acttgccctg ttatcgcaac
     5281 ccgcgctaca tatatccacc cacacgggca tattgagcga tttttgatat ttattcatct
     5341 ccttacgcac tatattcaga gcaataagtt gcctcttttt atcttcattc agcacaacct
     5401 ttgcaactaa aaggagccca tgatggttac agttgtcagc aattattgtc aattatctca
     5461 aacccaactc agtcagacat ttgcagaaaa atttactgtg accgaggaat tactgcagtc
     5521 tttaaaaaaa acagcgttat ccggagatga agaaagtatt gagttactgc ataatattgc
     5581 gttaggttat gatgaatttg gaaaaaaagc tgaagatatt ctttaccata ttgttagaaa
     5641 cccaacaaat gagaccctat cgatt
//
LOCUS       DAZGLJ010000269         5609 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000269 DAZGLJ010000000
VERSION     DAZGLJ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.269"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            80..790
                     /locus_tag="ACHK49_004453"
     CDS             80..790
                     /locus_tag="ACHK49_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO9264965.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            832..3483
                     /locus_tag="ACHK49_004454"
     CDS             832..3483
                     /locus_tag="ACHK49_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO9264966.1"
                     /translation="MTAFRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKIALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVITVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            3497..4027
                     /locus_tag="ACHK49_004455"
     CDS             3497..4027
                     /locus_tag="ACHK49_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9264967.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4040..4543
                     /locus_tag="ACHK49_004456"
     CDS             4040..4543
                     /locus_tag="ACHK49_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9264968.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            4602..5516
                     /locus_tag="ACHK49_004457"
     CDS             4602..5516
                     /locus_tag="ACHK49_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9264969.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         1616 a         1213 c         1254 g         1526 t
ORIGIN      
        1 aatgtattag caacattaat cgattgtctg atgtaacggt tttcccgctt atcctcttaa
       61 cacatggaga atgcatcaga tgcaaactac gcgtaccccc tactctattt cctttatggc
      121 gacagtgtta cttttattgc tctttgcttg ccatagcacc gttgccaacg cagctgtcgc
      181 actgggtgcg acgcgggtaa tttatccggc aaaccagaaa caggttttat tgcctgtcac
      241 gaataatgat cctgccagtg tctatctcat tcagtcatgg atagaaaatg caggtgacca
      301 aaaagacacc cagtttgtca ttaccccacc gcttttttcc atgcaaggta aaaaagagaa
      361 tacgctgcgg ataatcaacg ctactaatca tcagttacca ggcgaccgtg aaagcctgtt
      421 ctgggtgaat gttaaagcta ttcctgctat ggaaaaagac cagaagaatg agaatacatt
      481 acagctggca attattagcc gcataaaaat gttttatcgc cccacgaacc tggcaatggc
      541 acctgaagaa gcgcctgcaa tgcttcggtt tcgtcgcagc ggtagcaaac tcacgcttat
      601 taatccaacg ccctatttca ttaccgtaac aaatatgaaa gcaggaaata gcaatctccc
      661 aaacaccatg gtgccgccaa aaggtgaagt atcggtggat atcacgcatg ctgcaaccgg
      721 ggatatcagt tttcagacga tcaatgatta cggtgcatta actccgcgta ttaaggcaac
      781 catgcagtaa gccagtgacc taattaataa taaactcaat cggaattaat aatgacagcc
      841 tttcgtgccg catttaaggc atatcgtatg catcaggtgt taataatgcc tcgctttgca
      901 cggcttacca ttgcccttgg cttagcaact gcggtatttc ccgttgacgc agagtattat
      961 tttaatccac ggtttttaag caatgatctt gctgaatctg tcgatttatc cgcctttact
     1021 aagggacgcg aggcaccacc tggtacatat cgggtcgaca tttatttgaa cgacgaattt
     1081 atgactagtc gggatattac ttttattgca gatgataata acgcagaact catcccatgc
     1141 ctgagtacag atcttcttgt cagtcttggt ataaaaaaaa tagcgttatt agacaataaa
     1201 gaacattcgg cagaaaaaca tgtgccggac aacagcgcat gcacaccact tcaggatcgt
     1261 ttggccgacg catcaactga gtttgatgtt ggtcagcaac atttgtcact ctccgttccg
     1321 caaatttatg ttggcagaat ggctcgcggc tatgtttctc ctgatctgtg ggaagaagga
     1381 ataaatgctg ggcttctaaa ctatagtttt aatggtaatt ctattaataa tcgtagtaac
     1441 cataatgcag gaaaatccaa ctatgcatat ttgaatttac agagtggcat caacattggt
     1501 agttggcgac tgcgcgataa ctcaacgtgg agttataaca gtgggagtag caattcatct
     1561 gacagcaata aatggcagca tatcaatacg tcggctgaac gtgacattat tcccttacgc
     1621 tcacggttaa cggtaggtga tagttatacc gatggcgata tttttgatag tgtgaacttt
     1681 cgtggcctca aaataaattc aacagaagcg atgttgcccg atagccaaca tggtttcgct
     1741 ccggtgattc atggtattgc ccgtggaacc gcacaagtga gtgtaaaaca gaacggatac
     1801 gatgtttatc agactactgt tccacccggc ccttttacta ttgatgatat caactctgcg
     1861 gccaatggtg gtgatctgca agtaaccata aaagaggcag acggcagtat tcagacatta
     1921 tatgttcctt attcgtctgt tccggttctc caacgtgctg gatatacgcg ttatgcgctt
     1981 gccatggggg aatatcgtag tggaaataac ctgcaaagct ctcccaagtt catacaaggt
     2041 agcttgatgc atggtctgga aggaaactgg acaccttatg gcggaatgca aattgcagaa
     2101 gattatcagg ccttcaacct tggtattggg aaagatttag gactttttgg tgccttttct
     2161 ttcgatatca cgcaggccaa tacgacactt gcagatggca cccgtcacag tgggcaatcg
     2221 gttaaatccg tctacagcaa atccttctac cagacaggaa ccaatatcca ggtcgcagga
     2281 tatcgctatt ctacgcaagg tttttataac ttatccgaca gtgcctacag tcgaatgagt
     2341 ggttacaccg tcaagcctcc taccggagac accaatgagc agacacaatt tattgattat
     2401 tttaatctgt tctacagtaa gcgtggtcag gaacaaataa gcatctctca gcagcttgga
     2461 aattacggta cgacattttt cagtgccagt cgccaaagtt actggaacac gtcacgcagc
     2521 gaccagcaaa tatcatttgg attaaatgtg ccgtttggtg atattacgac ttcgctgaat
     2581 tacagctatt ccaataatat atggcaaaac gatcgggatc atttactcgc ttttacgctt
     2641 aatgttccct tcagtcattg gatgcgtaca gacagtcagt cggcatttcg taattcaaac
     2701 gccagttaca gtatgtcaaa cgatttgaaa ggcggcatga ccaatctatc gggggtttat
     2761 ggcactctgc tgccggataa taacctgaat tatagcgttc aggtcggtaa cacccacgga
     2821 ggtaatacat cgtctggcac cagtggttac agttctctta attatcgtgg agcttatggt
     2881 aatactaatg tcggttacag tcggagtggt gacagcagcc agatttatta cggaatgagt
     2941 ggtgggatta ttgctcatgc tgatggcatc acctttggac agccgctggg cgacacaatg
     3001 gttctggtta aggctcctgg tgctgataat gtcaaaatag agaaccagac cggaattcat
     3061 accgactggc gtggctatgc catattacca tttgcgacag aatatagaga aaaccgtgtt
     3121 gctcttaacg cgaattccct tgcagataat gttgaactgg atgaaaccgt gatcactgtc
     3181 atcccaactc acggtgctat tgccagagca acatttaatg cacaaatcgg cgggaaagta
     3241 ttaatgacgt tgaagtacgg taataagagt gttccattcg gtgcaattgt cacacacgga
     3301 gagaataaaa atggcagcat tgtcgcagaa aacggtcagg tttatctgac tggacttcca
     3361 cagtcaggga aattacaggt ttcatggggc aaagataaaa actcaaactg tattgtcgag
     3421 tacaagcttc ctgaagtttc tcctggtacc ttactgaacc agcagacagc aatctgtcgc
     3481 taaggaacaa tagattatga aatacaataa cattattttc ctcggtttat gtctggggtt
     3541 aaccacctat tctgctttat ccgcagatag cgttattaaa attagcgggc gcgtcctcga
     3601 ttatggctgc acagtctcat cggattcgct taattttacc gtagatctcc aaaaaaacag
     3661 tgccagacaa tttccaacga ccggtagcac aagtccagcc gtcccttttc agattacgtt
     3721 aagtgaatgc agcaaaggga caacgggggt tcgggttgca tttaacggta ttgaggatgc
     3781 agaaaataat actttgttga aactggatga aggaagcaat acggcctccg gtttgggtat
     3841 agaaatattg gacgcaaata tgcgtccggt gaaactgaat gaccttcatg ccgggatgca
     3901 gtggatccca ctggtaccag aacagaacaa tattttgcct tactccgctc gtctgaagtc
     3961 aactcagaag tccgtcaatc cgggactggt gagggcttcg gcaaccttta cccttgaatt
     4021 tcaataatca gagtcagata tgaaaagact tcacaagagg ttcctgttag ctacgttttg
     4081 cgcgttattc acagcaactc tccaggccgc cgatgtcact atcactgtta atggtcgggt
     4141 agtcgctaaa ccctgcacta ttcaaaccaa agaagctaac gttaatctcg gggatcttta
     4201 tacgcgcaat ctgcaacaac ctggttctgc atctggctgg cacaatatta ctttgtcatt
     4261 aaccgattgt ccggttgaaa caagtgcagt gacggcaatc gtgacaggtt caactgacaa
     4321 tacgggttat tacaaaaatg aaggtactgc cgaaaatatt cagatagagc tgagggatga
     4381 ccaggatgct gcgttaaaaa atggcgatag caaaacggtt attgttgatg agatcactcg
     4441 taatgcacag tttccactta aggcaagagc tatcacggtg aatggaaacg caagccaggg
     4501 aacgatcgag gcgctaatca atgtgatcta cacctggcaa taacgtcgat tcgattttct
     4561 tagccttctt tttaaatcaa tttttaacct taggatatat catgggtaaa acaatcagta
     4621 taaaggtcct gttcggtatt tatcttctat taatggcagg caaagttttt gccttcagtt
     4681 gtaatgtcga tggcgggtca agtattggag caggtacaac ctctgtttat gttaatctcg
     4741 accctgtaat acagccgggc cagaatctgg ttgtagactt gtctcagcat ataagttgct
     4801 ggaatgatta cggcggctgg tacgacactg atcatataaa cctggtacaa ggctcagctt
     4861 tcgcaggctc cctacagtca tataaaggat cgctgtactg gaataatgtg acctacccct
     4921 tcccgttgac gacaaatacc aacgtattgg atattggtga taagacaccg atgccattac
     4981 cgttaaaact ctatattacc ccagttgggg cggccggtgg agttgtcata aaagcggggg
     5041 aagtcattgc ccgtatacat atgtataaaa ttgcaacatt aggaagcgga aatccgcgta
     5101 attttacgtg gaatattatt tcaaataaca atgttgttat gccaacaggc ggctgcaccg
     5161 ttgatagtcg taatgtcacc gtggatctcc cggactttcc tggaagcgca gaaatacccc
     5221 ttggcgtata ttgctcaagc gaacaaaagc tctcatttta tctttcgggt gccaccactg
     5281 acagtagccg acaagtgttt gctaacacag cacctgatgc aacaaaagca tcgggagtgg
     5341 gtgtcactct aatgcgtaat ggaaaaatac tggctacggg agaaaatgtc tccctgggga
     5401 cagtaaataa aagcaaagta cctctgggtt taagcgcaac ctatggacaa acaggaaaca
     5461 aggtttcagc cggaaccgtc cagtcagtca ttggtgtcac ttttatctat gaataattgt
     5521 gattttgacc ttagtctatg cgatccattc acgaaaattg cgcggataat tattttgtga
     5581 aggctattag cctacacctg taagaaaat
//
LOCUS       DAZGLJ010000270         5570 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000270 DAZGLJ010000000
VERSION     DAZGLJ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.270"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(128..1408)
                     /gene="gabT"
                     /locus_tag="ACHK49_004458"
     CDS             complement(128..1408)
                     /gene="gabT"
                     /locus_tag="ACHK49_004458"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO9264970.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            complement(1422..2870)
                     /gene="gabD"
                     /locus_tag="ACHK49_004459"
     CDS             complement(1422..2870)
                     /gene="gabD"
                     /locus_tag="ACHK49_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO9264971.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPMVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            complement(2893..4161)
                     /gene="lhgO"
                     /locus_tag="ACHK49_004460"
     CDS             complement(2893..4161)
                     /gene="lhgO"
                     /locus_tag="ACHK49_004460"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO9264972.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            complement(4181..5158)
                     /gene="glaH"
                     /locus_tag="ACHK49_004461"
     CDS             complement(4181..5158)
                     /gene="glaH"
                     /locus_tag="ACHK49_004461"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO9264973.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
BASE COUNT         1218 a         1589 c         1484 g         1279 t
ORIGIN      
        1 tctccccaga ggagcgaggg gacagtccgc agtcgttttt aactttgtcg atcgcctcat
       61 cgttattatt ttttactgca tcgttatcat tcccttagca acgtttaact cttacaaacg
      121 gacatagcta ctgcttcgcc tcagcaaaac actggctgat gatctccaga ccctgacgga
      181 tctgagcgtc ttcaatggtg agcggtacaa ggatgcgcag cacgttgtaa tacgggccgc
      241 aggagagaag aatcaggccc ttatcgcggg cgcgtgccac gatctcggcg gtgagttttg
      301 cgtccggttt gctgtgatcg ccatcttcaa acagctcgat ggcaatcatc gctcccagcc
      361 cgcgtacgtc gccgatctcc gggtgttttt cggctatcgc caacaatccg tcttttagct
      421 tctgccccag atcgttggct ttttgcagca gattttcctg ctcaaacacc ttcaacactt
      481 ccagcgccgc cacgcaggca atcgggttac cggcataggt gccgcccaga ccgcctggag
      541 cgacggcatc catcacttcc gcgcgcccgg tgacgcccgc cagcgggaag ccgcccgcga
      601 tcgatttcgc aaaggtagtg agatccggcg caacgcccat ctgctccatc gcaaacagcg
      661 tgccggtacg ccccgcgccg ctctgcactt catcggcaat cagcatgatc ccgtgctcgt
      721 cacacagcgc gcgtaaacgc tgcataaagg ctggcgacgc ggcgtagaaa ccgccttcgc
      781 cctgaaccgg ctcaatcacg atggcggcga tatcttccgg cgcggcatca tttttgaaga
      841 tccggtggat gctggcgata gcgtcatcct cgcttatgcc gtgcagcggg caaggataaa
      901 gcgcgcgata aacatgaccc ggcatcagcc ccatgcccgc agagtacgga ttcaccttgc
      961 cggtcagcgc cagcgtgtaa tgcgtgcgcc cgtgatacgc gccgctaaaa gcgatggtgc
     1021 cgctacgttt ggtggcggcg cgggcgattt ttaccgcgtt ttccaccgct tcggaaccgg
     1081 tcgtaaccag cagcgttttc ttggcgaaat cgcccggcac cttctgattc ataatctcgc
     1141 acagctccag atacggctcg taagccagca cctggaagca ggtgtgcgac agttttttca
     1201 actgcgcttc caccgcggcc accaccttcg gatgcaggtg cccggtattg agcaccgcaa
     1261 tcccgcccgc gaaatcaaga tactcacggc cttcaacgtc ccacacccgg cagttttccg
     1321 cgcggtcagc gaaaatcggg tgaatttgcc caacgccacg gggaatcgcc tgactgcggc
     1381 gctgcattaa ctctttattg ctgttcattc gcattctcca gttaaagacc gatgcacata
     1441 tatttgattt ctaagtaatc ttcgatgcca tacttcgaac cttcacgacc cagacccgag
     1501 gctttgatgc cgccgaacgg ggccacttca ttggaaataa tgccggtatt gatgccgacg
     1561 atgccgtact ccagcgcttc gcccacgcgg aagacgcggc ttaaatcacg ggcgtagaaa
     1621 taggcggcaa ggccaaactc ggtgtcattg gcttgcgcaa tcacatcggc ttcatcttta
     1681 aagcggaaca gcggggcgag ggggccgaac gtctcttctt tcgacacttt ggcgttggcc
     1741 ggcacgtcca ccagaatggt cggctggaag aagttgccgc cgcgttcatc cgctttaccg
     1801 ccgcaaacca cacgcgcacc tttctccagc gcatcggcaa tatgctcttc cacttttgct
     1861 accgcttttt catcgatcag cggcccgatg gtgacgcctt tatccagccc gtcgccgatg
     1921 tgcagtttgc tcaccgcctg ctgcaatttt tcggcaaagc ggtcatacac gccgtcctgc
     1981 acgtataaac ggttggcgca gacgcaggtt tgcccggcgt tgcggaattt cgaggccagc
     2041 gcgccttcca cggctttatc gagatcggca tcgtcaaaga cgataaacgg cgcgttgccg
     2101 cccagctcca gcgacacttt tttgatgtct ttcgcgcact gttccattaa ctggcggcca
     2161 atttcggtcg aaccggtaaa cgacagtttg cgtaccatcg ggttgctggt cagttcatta
     2221 ccgaccgcgc ctgccgaacc ggtgaccacg ttaaacacgc cagccggaat gcccgcgcga
     2281 atcgccagct ccgccagcgc cagcgcagag aacggtgtct gactggcggg cttcagcacc
     2341 atggtgcagc ctgccgccag cgccggaccg gctttacggg taatcatcgc cgccgggaag
     2401 ttccacggcg tgatagctgc ggtaacgcca atcggctgct tgataacaat caggcgttta
     2461 tcggcctgat gaccgggaat ggtgtcgcca taaatgcgtt tgccttcttc ggcaaaccac
     2521 tcaataaaag aggcggcgta gctgatttca cctttcgctt cggccagtgg tttgccctgt
     2581 tcgagggtca tcaggcgcgc taaatcgtcc tgatgctcca tcatcaaatt gaaccagttg
     2641 cgcagaatgt tggcgcgttc tttggcggtg agcgcacgcc aggcgggcag ggcgcggttg
     2701 gcggcgtcga tagcggcgcg ggtttcatca gcgcccattt tcggcacgct acccagcttg
     2761 tcgccgttcg ccggattggt gacgtcgatg acttcaccat tgttggcgtc cagccattcc
     2821 ccgttaatca acgcctgctg gcggaataag ttactgtcgt taagtttcat tgttacgtcc
     2881 tgttttcaaa ggttattgat taaatgcggc gtgtaaggca tccacactac gtgccgctcg
     2941 cagcgtgcgt ccggggttac tctggcttgc caacagcgtt tgtaccttgc tgacaatatg
     3001 cgcaccaata ggaattgctg atgtcgctgc cggggagggc gcattgcagg tgtggatcgt
     3061 gcgtggggtg gtgacaaaca gaaaatcgtc aatcagcttg ccgtccggcg atactgcctg
     3121 cgcccgcaca ccggcgggcc agggctggag atcgcttaac gaaagccggg gacaatactt
     3181 ttgcaccagg cgcagatagc cgcttttgca cagcgagttt ttcatctcgc ccagtcctga
     3241 gcgtagatgg ttttgcagca cccggcgaat ccccgacgag cccaaaatct ccagcgtgtc
     3301 gctaaatgag aagtcgcgct tgcgatagcc ttcgcgtttg aaagccagca ccgcgtttgg
     3361 cccgacggtg acgctgccat cgatcattcg ggtgagatgc acgcccaaaa atggcattgc
     3421 cgggtcggga atggggtaaa tcagatggtt aacaatctgg ttatgctccg gcgcaaggcg
     3481 gaaatactcg ccacggaacg ggcagataat aaaacccggt tcgaggccga gcattttcac
     3541 cagccggtca gccatcagcc cggaacagct aatcagcgtc gatgcttcat attcgccgcc
     3601 ctgacgggtt cggacgacca cgccgttttt atgctcactg agggcgctga cttcggcgtt
     3661 ataaataatc tcaccgcctc tggcctggaa gatttttgcc attgccgctg tgacttcgcg
     3721 atagctgaca atgccgctgg acggcacaaa aatgccgccg agcccggtga tattcggttc
     3781 gcgctcgcgc agttcctcgg cgtttaacca ctcgcgctcg ataccgttcg ccgccgtgcg
     3841 ttcccacaac gcgcgcatcc gttccatttc gagatcggac gtggcgacca gcatcttgcc
     3901 gcagttgtca tagcgaatgt cgttttgatc gcaaaaggct ttggtggcgc ggtttcccgc
     3961 caggcaaaac tgcgccttca ggctgccggg cgtgtaatag accccggcat ggatcacgcc
     4021 gctgttgtgg cccgtctggt gacaggccgg gccggactct ttttccagca acgcaatgcg
     4081 ggcatccgga taaacatcaa tcagttgcat ggcggtcgac atgccgatga tgccgccgcc
     4141 aataatcaca aaatcataca tccgctcaat tcctttgcgc ttactgatgc gtctggtagt
     4201 ggtgagtggc gtaagcgaaa tagccacgct gacgcatcag ttcacggcgc agatccgggt
     4261 gcggagtaaa gcggtcgcga ccgtgcagcc agaacaggtt gtttatcaac aggaatttgc
     4321 caacgggaac gggtacagaa agaatgcctt tgctggtttc aatggcatcg gaaagctcgc
     4381 tcaaccacac gccttcttca aaatcttttg gctggacgaa ctggtcgata tagcgcatca
     4441 ccgggcgacc ctgttgatca acgtcgaaca ctggatggaa aacatctttg ctgacgtttt
     4501 tgctcggcgg cgcggcaaag cgcatcgggc gacgcgccag tggatggcgg aaatagtggt
     4561 ccagatgttc ccagtcatcg agatgcagca gcaacgaatt tccaccctgc atgttttgct
     4621 catcgatttt catcatcagc acgtaatcgg tgatctcttc gacatacgtg ccgtcgttgt
     4681 gcagttccat cacgcggtgc ggctgacgca gatagctgtc tgagttatcg acatttttca
     4741 ccacgaaacg cgcgtaatac tgaccgctca tcgcatcgaa atttgagcga ccaatcagat
     4801 gcgctaccgc cgttgccagc ttcaccatct catctgcctg cgccacatca tcgataccca
     4861 ccgcattgat caacagcgca ccttcagcac ggtttagcag ggtcttcaac agcaatggtt
     4921 gcagctgatt cgcacacaga tcgtcaagaa ttttgcctac ccgaaaacgc agaaacgatt
     4981 tgtactccag cgcctgcacg ggccattcgg caacctgttc gagaaactgt ttggtcgtct
     5041 gttcggtgaa ggtgagttcc agcagacgcg gggattgcgc cgacgggatg agagtgaatc
     5101 cgctatagtc ctggcctgaa tcgacagcgt tattgtgtac ggcggtcagt gcattcatca
     5161 gaagcgatcc tcttatgaga tgtagggtga catggcgatg ctcatttcgt agccataatc
     5221 taaaaatatc tacatttctg aaaaatgcgc acaaaagcga catattgttt tcttattgtg
     5281 atcaaaagca acaaattagt aacatttaac gtagggtatt aaggcaaagt gagacaggaa
     5341 atgaataact aaagcaacag gttaggggga gttaacatta gagaacagat tatctggtcg
     5401 ataaaaaaca gtaaatggct tcactgcaaa atgaccgtta gtaaactaaa catattatct
     5461 gccccgtttt atcggggcag atataacatt aaattataaa tagagatagt tcggcggttc
     5521 tcctgttttc cctaaaagaa gagcgctacg cccaggaata ttgatattta
//
LOCUS       DAZGLJ010000271         5533 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000271 DAZGLJ010000000
VERSION     DAZGLJ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.271"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(325..1113)
                     /gene="phoH"
                     /locus_tag="ACHK49_004462"
     CDS             complement(325..1113)
                     /gene="phoH"
                     /locus_tag="ACHK49_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO9264974.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1732..3003)
                     /gene="efeB"
                     /locus_tag="ACHK49_004463"
     CDS             complement(1732..3003)
                     /gene="efeB"
                     /locus_tag="ACHK49_004463"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO9264975.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(3009..4136)
                     /gene="efeO"
                     /locus_tag="ACHK49_004464"
     CDS             complement(3009..4136)
                     /gene="efeO"
                     /locus_tag="ACHK49_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO9264976.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVTQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(4194..5024)
                     /gene="efeU"
                     /locus_tag="ACHK49_004465"
     CDS             complement(4194..5024)
                     /gene="efeU"
                     /locus_tag="ACHK49_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO9264977.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         1391 a         1518 c         1309 g         1315 t
ORIGIN      
        1 gttaaacagt cagtcaaagt atagcccatt gttgattaaa cctcataagc aaaattggca
       61 ccagatctcc tgtacaggtt gaatggccga aaaaagatag gcagatgctc atttttttgg
      121 cttctggaag aaaaactctc cgcgctggca gtattacact ggtctgatgc tatgacgaaa
      181 aatgcctggt aagagtatct aaaccagact ccagttcagc ttatcaacac gctcaaagtc
      241 gcccccctga acgctaacct gaagtgaaaa tctgttgaaa taaaacaaag cccggttcgc
      301 ccgggctctg caccgataat acacttaact gtaggcatgc agcgtacgtt ggcaaagtgc
      361 cgaacgtacg cagtcctctt taccgaaccg gacgatccca accatttcat cttcttcgaa
      421 acgttccagc gcgtcactta atccggagca cacgccgcga ggcaaatcgc attgcgtgat
      481 atcaccgttg acgataaccg tcacgttctc cccgaggcgg gttaaaaaca ttttcatttg
      541 cgcggcagtc acattctgcg cctcgtcaag aatgacgact gcattttcaa aggtacgtcc
      601 acgcatatag gcgaacggcg caatttccac cttccctatt tccggtcgca ggcagtactg
      661 cataaaggaa gcccctaagc gccggaccag cacgtcgtag accgggcgaa aatagggagc
      721 aaacttttct gcgatatctc caggtaagaa gccaagatct tcatcagctt gcagaactgg
      781 acgggtgacg ataatcctgt cgacatcctt atgtatcagg gcttctgccg cttttgctgc
      841 gctgatccag gtttttccgc acccggcttc gcccgtggcg aatatcagct gcttactctc
      901 aatagccttc agatagtgca attgcgcttc atttcgcgcg aggatgggcg aagtatcgcg
      961 actgtcgcgg gccataccaa tggcttctac gccgcccatc tgcacaagcg aggtgaccga
     1021 ttcttcttca cgctgcttat ggctgcgcga atcccgtctc agcacacgtt ttgcctcgcg
     1081 acgagctttg atcactgctt tttgtcttcc catggagagc accttgagtt gtttgtattc
     1141 atcacacgcg ccgttggcag cgcgattatg cgcacgaaca tcagagggtt ggcttccttg
     1201 taagccatag tttgcttttg gataaaatgc cgaaaaacgg ctacgcacac cgtttgcggc
     1261 gtcggtaaca cgagtaaaga gaggaagtga gaaaaataca gagtgacgaa gttgctaccg
     1321 gaggagaaat ctccgcaagt ggtggagcgt agcttaacaa gacattgtcc agaacaggac
     1381 gttaccatcc gcgatctcca tagtgactga ctatcactgc cgggaacttc cgctgctact
     1441 taataattac aacagatctc gcattcattg caacaatata ttcacttata tttaaccata
     1501 aaacaccatt tcagtgacat tagtttctac tggaaagatg acagagtgat gacagatatg
     1561 aaaaaagctg tgtgctttca gcaggattcg catcaggcat ctgcgcacga acgccggatg
     1621 cggcgtgaac gccttatccg cctaccgaac ccgcaccgat tgtaggcata agaagacgcg
     1681 caagcatcgc atcaggcatc tgcgcaagaa cgccttatcc gtctataaac attaaacccg
     1741 caataacgcg cttccgagat aatcgttcgc gtccttcact cccggcagcg caaaaaaata
     1801 accgccgccg ataggtttaa cgtattcctc cagcgcttcg ccattgagcc ttttttgtac
     1861 tgtcaggaag cctttttcca gatcgtgttg gtaacagaca aacagtagcc ccatatccag
     1921 ttgcccggag ttggtgacgc ccagtgaata actgtagcca cgacgcagca tcaggctgga
     1981 ctcactctcc gccgtgcggg gattcgccag ccggatatgg ctgtccagcg caatcacctt
     2041 cccttccgga tcgctggcgt aatcgggaac atcatgctca tgctgcattc ccagcggcgc
     2101 accggtttgc ttatcacgac caaaaatcgt ctgctgttct ttcagcggag ttctgtccca
     2161 aaattccact cgaaactgaa tcaagcgtac tgcctgatag ctgccaccga ttgtccacgc
     2221 tggctcctgc tgatctgccg ttacccacac cactttttgc atcaacttat cattctggct
     2281 atcgggattg gcagtgccgt ctttgaaacc cagcaaatta atcggcgtct ctttgccttt
     2341 actacgcgcc gcgtgatcgg aaataaaccc ttcccgcttc cagcgcacgc tgagcaaatc
     2401 cggcgtatgt ttgatgatat cgcgcagtgc atggatcacc gtgtcctggg tgttggcgca
     2461 aatttgtagc aacacatcac catgacataa cgccgcatcc agcgagtcgt tgggaaaacg
     2521 cgtcatcttc tgcaactttt tcggcatctg tggcgcaagg ccaaagcgct catcaaacaa
     2581 tgagtgaccc accgataacg tgatggtgag attatcgggc gcaatgtagc cgccaagaat
     2641 gccggaatcg agtggtggca ggcgcggatt tggcgtttct ggtgccgctc cgccctgagt
     2701 cagaaaagca aaacgctgag tcaacaagcg aaacaaccgt tcaagatcgg ctttatcgct
     2761 ggcaagcaca tcaaacgcca ccagcatcat tgcggcctgt tgtggcgtca ggatccctgc
     2821 ctgatgctca ccataaaacg gctgtttctc attgcgtgca tccggtgaaa gcgtacccgg
     2881 cgcactttgc gttttttgtg catgagcgac cggacaactt cccgccagcg ccagcgcacc
     2941 aatgcctttc agtaaacgtc ggcgtgacgg ttcattcacg ccgtttttat cttcatactg
     3001 cataatgctt aatccaatcc cagcacaccg cgaagttgcg ccagatcttc cgccagcgca
     3061 gtaatcggtc ctttcagtgc attccggtcg gcatcggtca atttgtcgta ggtttcaaaa
     3121 ccgtctttag tacggtattt cgccagaatg gtatcgacct ttttaaagtt ggcatcgact
     3181 tttgccaaca gttccgggtt ggctttttgc agttgtggac gcagcagatc gacaattttc
     3241 tgcgagcctt caacgttagc ctggaaatcc cacagatcgg tgtggctgta gcgatcttct
     3301 tcaccgctga ttttgctggc tgccacttcc tcaatcagtc cggctgcacc gccgaccact
     3361 tttgaaggtg ggaaagccag ttcactgatg cgtttttgca aatcgaccac atcggtataa
     3421 agctggtcag cgtactgatc catgcctttg gtggtgttgt cgccaaacaa tgctttttcc
     3481 agacggtgga aaccggtgaa ttttggatca gcggcttttt gctcgtaatc atcttcacgg
     3541 gcgtcaatgc tgccatccag atcggagaac agttcagcaa tcggttcaat gcgctcatag
     3601 tgctggcgcg tcggcgcata cagtgctttc gctttttcga tatcgcctgc tttaatcgcg
     3661 tcggtaaagg ctttggtgtc ggtcaccagc tgcgtggttt ccgccatgac atacgcttta
     3721 tatgcagtaa ttgcaccacc aagacttaac agcgcatcac tttgcgccgc atccgccgtt
     3781 gcctcacctt tgacgatcaa cttccctttc gggttagtca gcagaccgca ggtcatatcg
     3841 tattcgccag gctgtaaatt cgccgtcatt ttctggctaa aaccaggggc gatattttcc
     3901 cgctcttcca ccaccatcac gcctttgagg atctcccact ccagcgcctt ctggctatgg
     3961 ttctgaataa tgaactgtgt tttcccggcg ttaaccgtaa tggtcatcgg ttcgcactgc
     4021 ttatccgtca cggtcacttt gacctgcgtc acatcagcgg cgttagccat aaaagcagaa
     4081 gaaaacagcg cagccacgct caactgcaat gcgttacggc ggaagttaat ggtcatgacg
     4141 agactatccc tttaaagtat gttgtaacta agcaagagtt tgcgtcgtat ttgttacgcg
     4201 gagcgagacg ctgtcgcccc tgcgcgtggt ggcagagcaa atgccaccag cgccgggatg
     4261 agataaataa accagacggc gacttcgctg acgctcggcg cttcctgata gccaaaaatc
     4321 ccttccatca gcgtgccaaa cagcgagtga gttgagagca ccgcactcat atcgaaggcg
     4381 atttcctgaa agtggttcca caatccggct tcatgaaatg cgcgaatggc accagctgcc
     4441 agccctgcgg cgacgaagag aataaacagg ctggtccatt taaaaaatgc accaagattg
     4501 aggcgaatac cgccccagta gagcaagaag cctagcacca cggcagtagc aagaccgagc
     4561 attgcaccca gcggcggcca gatcccgaca tcttgttgaa atgccgccag caggaaaaag
     4621 accgactcca gcccttccct tgcaacggca aaaaagacca tcatcaccag cgcccagcca
     4681 tgatgatttc cacgctgcaa tgcgctatcg actgcctgct ccagttgtac tttgacgttg
     4741 cgcgacactt tgcgcatcca gaaaaccatc caggtaagga tcaccacggc gatcaccgcc
     4801 acgatacctt caaacagttc ctgctctttt tgcggaaatt cgccggtggt ttcgttaatg
     4861 aagatcccca agcccaggca caacgcagcg gcaagcaaca cgccaatcca catcacacca
     4921 atccatcggc ctcgctgggt acgcttaaga tagctggcaa tcaaactgac aatcagcgcg
     4981 gcttcaagtc cttcgcgcaa cataatgaga aacggaacaa acatgccacc catccttaaa
     5041 cgtttttctg agtcaatcta tgcaaagaaa agtaaatcgc agtgatagcg attatcatta
     5101 cggtaaagaa aaatacaagc ggaatgttta taaaatgatg ttgaaatgta aacaaagaga
     5161 ggtaatgcca ggggatataa acgattattc caggtacaaa gatgggattg tttacaacga
     5221 gccatgtgtt cttgccggat gccgcgtaaa cgccgtatcc agccgacaaa accgtcctga
     5281 ctcaatacat caaaacttcc atgtaaaact gataagcggg tgtatcaagc aatttcgtcc
     5341 atcctcgatt atcaaatcta actttttgcc cggaacataa gaacagcgcg ggtgttggtg
     5401 gcgtcaaaat cgctgaaacg ttccccgaag gccggggcag cccacatgga tgtgggctga
     5461 gggcgcgtct tacaggacgt tacctcgcgc ccgacccggt agccgaaggg gataagctga
     5521 aggtaccacg cag
//
LOCUS       DAZGLJ010000272         5485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000272 DAZGLJ010000000
VERSION     DAZGLJ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.272"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(126..911)
                     /gene="ygcW"
                     /locus_tag="ACHK49_004466"
                     /pseudo
     CDS             complement(126..911)
                     /gene="ygcW"
                     /locus_tag="ACHK49_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            1229..2506
                     /gene="yqcE"
                     /locus_tag="ACHK49_004467"
     CDS             1229..2506
                     /gene="yqcE"
                     /locus_tag="ACHK49_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO9264978.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEVRTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            2533..4011
                     /gene="ygcE"
                     /locus_tag="ACHK49_004468"
     CDS             2533..4011
                     /gene="ygcE"
                     /locus_tag="ACHK49_004468"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO9264979.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGSVVCEGKGLLQPMHTPYAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
     repeat_region   4650..5223
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4711..4735
                     /rpt_unit_seq="cggtttatccccgctggcgcgggga"
BASE COUNT         1493 a         1237 c         1289 g         1466 t
ORIGIN      
        1 tctgcaccaa caatttcctt tagttggtcg acaatcgctg cgcgagataa agacatagta
       61 attccttcct gataataaaa atggaataat cattaataag agaaaacaga aaccaccgtt
      121 atggattagc gcactaaata accaccatcg accactaata aatgcccatt gacataattt
      181 gaagccggac ttgcgaggaa tacagctgcg cccattaaat cctgagtatc gccccaacgg
      241 tttgccggaa tatgatcaag aacgcgctga ttggtttctg gattactgcg tgtcgccagc
      301 gtaatatctg ttgcataata gccaggggca ataccattta cctgaatatt atattgacct
      361 agttcatcac aataagcttt ggtgaatccg gcaagagcat gtttagtggc agaatatgca
      421 ggtgaccatt gtccacctaa gtaagagaac aatgaacaga tattaataat tttgccgctt
      481 ttctgcggga tcataatttt tgcagcttca tagcttaact cgaatgcggc ggtcaggttc
      541 acatcaatca tcggatccca gtcggcacga ccgaagtcca gcaccttatt cagcttacaa
      601 ataccggcat tgtttaccag aatatcaact gtaccgaaac gctcacagca agcagcgata
      661 atcttctgcg gcgcgccttc tgcggtgata tccacctgca tgaagtccac ctcaacaccc
      721 tgtttttcaa tcatttcctt tgtttcgccg ttatctttga cgaaactagg aataaagata
      781 tttgcgccag ctttggccaa cgccatggca aatgcctagc ctaaaccgct attcccaccg
      841 gtaacaattg cggttttacc tttcagtgag aaaaaatcca ttgagaacgc attgagagat
      901 tcgattgaca tagttagctc caatttccgt aaggcaaaaa aaagaaaggt aacttccccc
      961 gcgagaggga agttaccttt ctcattatct ctggtaactg gagataattt ttagcataac
     1021 ggcataactg ctaatgtgat cgtaatcaca gtgtgatatt cgtattgtca aatagtttta
     1081 aaaagcagaa gcgtgattac tctcacacaa cacctctgct ttccatgtta tttcttattg
     1141 caattactag agacctgaga aaagtaatcc acctgaaagg gcggcttact tttctctttt
     1201 ttttgtctat tacaaatggg aatacgatat gcaacacaac tcatatcgcc gttggataac
     1261 cctcgcgata attagtttta gcggcggcgt tagttttgac ctggcttatt tacgttatat
     1321 ttatcaaatt cccatggcga aatttatggg attcagcaat accgagatag gtttaataat
     1381 gagtaccttt ggtattgcgg ctattattct ttatgccccc agcggcgtta ttgccgataa
     1441 attttcacac cgcaaaatga ttacttccgc gatgatcatt accggattac tgggtctgtt
     1501 aatggcaacg tatccaccgc tgtgggtaat gctctgtatt caggtcgcct ttgcgataac
     1561 gacgatttta atgctgtggt cggtgtcgat taaagccgcc tcgttgctgg gcgatcatag
     1621 tgagcaaggg aaaattatgg gctggatgga agggctgcgc ggcgtcggtg taatgtcgct
     1681 ggcggtgttt accatgtggg tcttttctcg ctttgcaccg gatgacagcg ccagcctgaa
     1741 aacagtcatt atcatctaca gtgtggttta catcttgttg gggattctgt gctggttttt
     1801 tgttagcgat aacaacaacc tgcgcagtgc caataacgaa gaaaaacagt cattccagct
     1861 tagcgacatc cttgccgttt tgcgtatcag caccacctgg tattgcagca tggtgatttt
     1921 tggtgtcttc acaatctacg ccattctgag ttactccacc aactatctga ccgaaatgta
     1981 cggcatgtcg ctggtggcag cgagctacat ggggattgtg atcaacaaaa tcttccgcgc
     2041 gctgtgcggc ccacttggcg gcattatcac cacctacagt aaagtgaaat cccctacccg
     2101 cgtgatccaa atcctttccg tactcggcct gctggcgtta actgccctgc tcgtcacgaa
     2161 ctctaacccg caatcggtcg cgatggggat tggcctgatt ttactgctgg gattcacctg
     2221 ttacgcctca cgcgggctgt actgggcctg ccctggcgaa gtgagaacac cgtcttacat
     2281 tatgggcacc acggtaggta tttgttcggt gattggattc ctgccggatg tcttcgttta
     2341 cccgattatc ggccactggc aagacactct gcccgcagca gaagcctacc gcaatatgtg
     2401 gctgatggga atggcggcgc ttgccatggt gattgtcttt acctttttgc tgttccaaaa
     2461 aattcgtact gctgatagcg cccccgcaat ggctagcagc aagtaagcca ggaaaattac
     2521 aggagaaaaa tcatgtcgaa aaaatacatc atagggattg atggcggaag tcagagcaca
     2581 aaagtggtga tgtacgatct ggaaggtagt gtggtttgcg aaggtaaagg cttattacag
     2641 ccgatgcaca cgccttatgc cgatactgca gaacatcctg acgacgattt atgggcatca
     2701 ttatgttttg caggtcacga tttgatgagc cagtttgccg ggaataaaga agatattgtt
     2761 ggcatcggcc tgggctccat ccgttgctgc cgtgcgttat tgaaagccga tggcacgcct
     2821 gctgcgccgt tgattagctg gcaggatgca cgcgttacac gcccttacga acatacgaat
     2881 cctgacgtgg cgtatgtcac ctctttttcg ggttatctga cgcatcgctt aaccggcgag
     2941 tttaaagaca atatcgccaa ctattttggt cagtggccgg tggattataa gagctgggca
     3001 tggagcgaag atgctgcggt aatggataag tttaatatcc cccgtcatat gctgtttgat
     3061 gtgcaaatgc ctggcaccgt cctcggacat atcacaccac aagccgcact ggcgacacat
     3121 ttcccggcag gactgccggt tgtttgtacc accagtgata aaccggtaga agctctgggg
     3181 gccggattac tggatgatga aactgcggta atttctcttg gcacttacat cgcattgatg
     3241 atgaacggca aagcactgcc gaaagatccg gtggcgtact ggccgattat gtcttctatt
     3301 ccgcaaacat tgctgtatga aggttacggt attcgcaaag gtatgtggac ggtgagctgg
     3361 ctgcgtgaca tgttaggcga gtcgttaatt caggatgcca gggcgcagga tctttcaccg
     3421 gaagatttac tcaacaagaa agcctctagt gtgccacctg gctgtaatgg gctgatgacg
     3481 gtgctggact ggctgaccaa tccgtgggaa ccgtacaaac gcgggattat gatcggcttt
     3541 gattccagca tggattacgc atggatatat cgttcgatat tggaaagcgt ggcgctaacg
     3601 ctgaagaaca attacgacaa tatgtgtaat gaaatgaatc actttgcgaa gcatgtgatc
     3661 attactggcg gcggttcgaa cagcgatctg tttatgcaaa tttttgccga cgtgttcaac
     3721 cttccggcac gccgtaacgc cattaacggt tgtgcaagtc tgggagcagc gattaataca
     3781 gcggtaggtc tggggctata cccggattac gcaacggctg ttgataagat ggttcgcgtg
     3841 aaagatatct ttataccgat tgagagcaat gccaaacgct acgacgcgat gaataaaggc
     3901 attttcaaag acctaaccaa acatactgat gtgatcctga aaaaatcgta tgaagtgatg
     3961 catggggaat tggggaatgt ggattcgatc cagagctggt cgaatgcgtg aatgaggtag
     4021 gccgggcgga aaactgcccg gcctgaacat acctaaatgg ttatccccgc tgacgcgggg
     4081 aacataagtt cgacaactta aaaaatgata acaagtccgg tttatccccg ctgatgcagg
     4141 gaacataata aaaactttaa ctagttcggt ttatccctgt ttttacaagg aatattaact
     4201 acactcgatg tctttagaat taaagacaat acgaatcgca ctttatcaat tttcacttaa
     4261 agtatgaact ggatatcttt attcataaga actattcatc aacagcgtat agaggcggtt
     4321 atgtaaaacc actcattagc ctcaaaacaa agacaaactt ccagatttat tacttaacca
     4381 atgaataaca agcttaaaaa atatactgtt attctaaaga aaaaattaac aggcattaat
     4441 tcaatattaa aaaaataata gattaaaatt tcttaacgat ttaagaatca tacaaataac
     4501 actttgatta aatttaattt tttgtatcga taattgtgaa cctctctggc atggagaact
     4561 attttgaaca tgaggtgtta cgtggatatg ttgcttatta caagtactgc taatataaaa
     4621 acttgagaaa gagataacgg gttatatggt ggtttatccc cgctggcgcg gggagctcaa
     4681 catcggaaac ggcttcgcgg cggcggcgtc cggtttatcc ccgctggcgc ggggaactcg
     4741 acagaacggc ctcagtagtc tcgtcaggct ccggtttatc cccgctggcg cggggagctc
     4801 tacgtgaaga atatttgcaa cacccgcaag aacggtttat ccccgctggc gcggggagct
     4861 cggcatagcc aggctgatcc ggcgacggcc ttacggttta tccccgctgg cgcggggaac
     4921 acaatcgtgt gtaaattcgc ggcggctcca ctggcggttt atccccgctg gcgcggggaa
     4981 cactggtcga aatatagaca gcatgttccg taccacggtt tatccccgct ggcgcgggga
     5041 acacacacta tccgggcggt attacgccca aatatccggt ttatccccgc tggcgcgggg
     5101 aacacctgcc gggtgaaacc actcgcggca gatcttgcgg tttatccccg ctggcgcggg
     5161 gaacacacta ctgtcggtag ctgggaggat gaggagatcg gtttatcccc gctggcgcgg
     5221 ggaacactct aaacataacc tattattaat taatgatttt ttaagccagt cacaatctac
     5281 caactttata gtatcacaca aacaacacat ccattatgtt aaagagcact taatccattg
     5341 attaaaaagg taaatattta aaataactct atacaaacta aaatctacca aactttaccg
     5401 caataatttt cactccagcg aaaaattaat gccacagaat ttgtagagaa tgctaatgat
     5461 tagccctgga tgggtaaaat ggagg
//
LOCUS       DAZGLJ010000273         5460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000273 DAZGLJ010000000
VERSION     DAZGLJ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.273"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..727
                     /gene="nanM"
                     /locus_tag="ACHK49_004469"
     CDS             <1..727
                     /gene="nanM"
                     /locus_tag="ACHK49_004469"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO9264980.1"
                     /translation="MAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNEAGKDSTAIDKI
                     NAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNKGDKTWLINGEA
                     KPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIFAGGAGFKGSRE
                     NYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPWNNSLLIIGGET
                     AGGKAVTDSVLISVKDNKVTVQN"
     gene            792..1772
                     /gene="nanS"
                     /locus_tag="ACHK49_004470"
     CDS             792..1772
                     /gene="nanS"
                     /locus_tag="ACHK49_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO9264981.1"
                     /translation="MNAIISPDYYYILTIAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPPCHFNDMIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPHNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDKFVEAILQFWRER"
     gene            complement(1780..2430)
                     /locus_tag="ACHK49_004471"
     CDS             complement(1780..2430)
                     /locus_tag="ACHK49_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO9264982.1"
                     /translation="MNKLPDHLPRRYIIEAIHLWDAGETHQFQPARLYEIEYHGRRYP
                     SKAIAGIAATLMTGTQFTPADFTGGIKSKCVKLLIEQGFHIHLDKAAAAPIADVLFPD
                     ELPSQETYLEGAAIKVTVNRYERDKKARDKAVEHHGCQCNVCGVDLVKIYGDIAEGFI
                     HVHHLVPLSAIKEDYQLDPVNDLLPVYPNCHAMLHRRKPPFTPEQLKALMDANKSN"
     gene            2567..2710
                     /locus_tag="ACHK49_004472"
                     /pseudo
     CDS             2567..2710
                     /locus_tag="ACHK49_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(3374..3502)
                     /locus_tag="ACHK49_004473"
                     /pseudo
     CDS             complement(3374..3502)
                     /locus_tag="ACHK49_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(3478..3627)
                     /locus_tag="ACHK49_004474"
                     /pseudo
     CDS             complement(3478..3627)
                     /locus_tag="ACHK49_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
     gene            complement(3712..3909)
                     /locus_tag="ACHK49_004475"
     CDS             complement(3712..3909)
                     /locus_tag="ACHK49_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO9264983.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            complement(3921..4409)
                     /locus_tag="ACHK49_004476"
     CDS             complement(3921..4409)
                     /locus_tag="ACHK49_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO9264984.1"
                     /translation="MKLSLMLEADRINVQALNMGRIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(4406..4780)
                     /locus_tag="ACHK49_004477"
     CDS             complement(4406..4780)
                     /locus_tag="ACHK49_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO9264985.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            complement(4870..5238)
                     /locus_tag="ACHK49_004478"
     CDS             complement(4870..5238)
                     /locus_tag="ACHK49_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO9264986.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
BASE COUNT         1456 a         1255 c         1358 g         1391 t
ORIGIN      
        1 catggcgggt catgtaactt ttgtacacaa cggcaaggct tatgttactg gcggtgttaa
       61 ccagaatatc ttcaatggct attttgaaga tctcaacgag gctggaaaag attcaaccgc
      121 tatagataaa atcaacgctc actattttga caaaaaagca gaagattatt ttttcaataa
      181 gtttctgttg tcttttgatc cctcaacaca gcaatggagt tacgctggcg aatcgccctg
      241 gtacggaacg gctggtgcgg cggttgtgaa taaaggtgat aaaacctggc ttattaatgg
      301 cgaagccaaa ccaggattgc gaacggatgc cgtatttgaa cttgatttca ccggtaataa
      361 tttaaaatgg aataagcttg ctcccgtctc atcaccagat ggcgtagctg gcggttttgc
      421 ggggataagc aatgattctc ttatatttgc cggaggggcc ggattcaaag gttcacgaga
      481 aaattaccag aacggtaaga actatgcgca tgaaggcctg aaaaaatcat atagcactga
      541 tattcatctt tggcataacg ggaaatggga taaatcgggt gaattatcgc aaggtcgggc
      601 ctacggagta tcattgccct ggaataatag tctattgatt attggcggtg aaactgcagg
      661 cggcaaagcg gtgacggatt cagttttgat ctctgtgaag gataataaag ttacagtaca
      721 aaactaacgc ttcagggccc cggtaaaggg gccctgctat caatttgtca tttcaattac
      781 gcgaaattta tatgaacgca ataatatcgc ccgattatta ctatattctt accattgctg
      841 gtcagtctaa tgccatggcg tatggcgaag gactgccatt accggacagg gaagatgcgc
      901 ctcatcccag aatcaaacaa ttagcgagat ttgcgcatac gcatcccgga ggcccgccat
      961 gtcactttaa cgacatgatt ccactgactc actgcccaca cgatgttcag gatatgcagg
     1021 gttatcacca tcctctggca acgaatcatc aaacacagta cggcaccgtt ggccaggcac
     1081 tgcatatagc acggaaatta ctgcccttta ttcctgataa tgcaggggtt ctcatcgttc
     1141 cgtgttgccg tggcggatcg gcttttaccg cgggcagcga agggacatat tcagaacggc
     1201 acggagccag ccatgatgct tgtcgttggg gaacggatac tccgctatac caggatttag
     1261 tcagcagaac gcgagccgca ctggcaaaaa atccgcataa caaattcctc ggcgtatgct
     1321 ggatgcaagg cgaatttgac ttaatgacca gtgactacgc gtcacaccct caacacttta
     1381 atcatatggt tgaagccttt cgtagggatc taaaacaata ccattctcag cttaataata
     1441 ttactgacgc accgtggttt tgcggcgata ccacctggta ctggaaagaa aatttccctc
     1501 atgcgtatga agttatttat ggcaattatc aaaataatgt tttagccaat attattttcg
     1561 tcgacttcca gcaacaaggt gaaagaggac tgacgaacgc gcctgatgaa gacccggacg
     1621 atttaagcac gggatattac ggttcagcgt accggtcacc ggagaactgg acgacggcac
     1681 tgcgaagcag tcatttcagc acggcagccc gtcgggggat tatttctgac aagtttgtag
     1741 aagcaatttt gcagttttgg cgcgaaaggt gagcgttcat taatttgatt tattcgcatc
     1801 catcagcgct tttaactgct ccggcgtaaa cggtggtttg cgtcggtgca acatcgcatg
     1861 gcaattaggg tacacaggca gcaaatcatt aactggatcc agctggtaat cctctttaat
     1921 ggctgacagc ggcaccaggt gatgcacatg gataaagcct tcagcgatat caccatagat
     1981 cttcaccaga tcaacaccgc atacgttgca ctggcatcca tgatgttcga ccgctttatc
     2041 tcgggccttt ttatcgcgtt catagcggtt tactgtcacc tttatcgccg caccttcaag
     2101 gtatgtctcc tgcgacggca actcatccgg gaacagaacg tcggctatgg gagccgcagc
     2161 ggctttatcc agatgaatat gaaatccctg ctctatgagt agcttgacac atttagattt
     2221 tattccgccg gtgaaatccg caggggtaaa ttgcgtcccc gtcatcaatg tcgctgcgat
     2281 gccagcaatc gccttcgagg ggtaacgtcg gccatgatat tcaatctcat acaaccgcgc
     2341 aggctggaac tgatgagttt ctcccgcatc ccataaatgg atagcttcga tgatataccg
     2401 ccgcggaaga tgatcaggga gtttattcat ggagaatgtc cttatacgtt ctcagaacag
     2461 gcagcaaaaa aacaaatgag atcaaggtaa tatatcttca cttgcaggct tgaacagcag
     2521 aaaatttgcg agcatgatcg caattatttc actgccaggg atatcaatag cttccagtaa
     2581 atcgctacag caggcaattg ccaatataaa aatctggcac aagggtgaac agcgcgcgcc
     2641 gcataagcca ttgttattgc tatacgtatt agcgggatac ctgaatggac atccgcgcct
     2701 tttcgattat tgtagtggaa cagtctacga ggatgcagtt cgaagtgagt aagtaaaggt
     2761 tgcgggaaac gatcaatgcc gccatacaat gattttttag gatcaacaat gagtaataaa
     2821 aactacgaaa gtcaccgcaa agcgattgtt agtaaaggta taccacccgc tctattaaat
     2881 aggctcacca attcggatgt tcaggtgatc aataccttct taacacgagt gagcaaactg
     2941 gaactatctc aacaagagaa agactggatc ataaaaatca tctctatggt ttagacaatt
     3001 tcagccttgt agcctttcaa gggttttgtg tgggtacaaa cattatcgaa tcggggtata
     3061 gccgcagctg tagctgtatc cacatcagta agttgatgta ggttgactta ggttgagcga
     3121 aaaatagcga aaagccttgt gtggcgcgga tttcagtcat aaaaaaacgt ccgttgatgt
     3181 ctattgatgt tccgatggtg cgaaggtgcg aaggtgcgaa ggccggactc aaacatgtga
     3241 cttaacctca tgatttaaaa ttgttaataa taaacaatat catcttatac caacacagat
     3301 accaacacga aaaatcactg gttttcggca tcgaaccagg acaatcagct cctgattgag
     3361 ataccgagct ctgttactcg tcatcttcct cgacaggctt cattgcatca agtagtactc
     3421 gcaccatcgc gatagaacca atccggataa aagcatcttc tccatctata tctgttaact
     3481 gatatcgctc acttgatgcc ggataattgt gtcgatttgc agcagtgaac tctttcgtac
     3541 agccagaatg cggcggatgc tggttgagca gataacgtcc gtattctcgt tgaccacttc
     3601 cggacggtag gctgtgatat cagacataat cgatattcct ttttgcagat aaatgaaaat
     3661 gccagccctg ttcaggctgg cgtcagtgga atgaagtctg gtgagtaaga ctcaccggtt
     3721 ctggtgctga agctgcagtt ggcgcagggt acggatatcc tctctggcct gttcgataca
     3781 gggcaacagt gctgctgaat ccgttttatc ctcattgttg tcaaagataa ttcccgattc
     3841 gcagtcgata ttgtcctgca gccacgcaat cagaatatcc agtgcggttt ccgtggttaa
     3901 tgatttcatg ttgttcctgg ttaccagttg aaagtgggta aacccggcag gcattcagcg
     3961 ctggcatcca gatgaatgag actgacgcca taacgtcggg taagtgtgat aaccagacga
     4021 cggaacgttt ttgacagccc caggcgttta agccgcagaa ccggatatga ccacgcatcg
     4081 gtacgtaaca gatatccgct acctgtgaaa tgaatccatt ctgattcacc aacgtcactg
     4141 gtctgatgcg acagcgagta cagccaggcg ttatcctttt ccgtgatatg tgcggtactg
     4201 cagcgtatgc cggcaagggt tgcaaacggt ggtagtgtgc aggttgactg ttggtcggat
     4261 tcctccacca cgcggagtga ataaccgttt tcagcgacct tgttaatcag ttcagtgaga
     4321 ttaacaccat cgacgtcaac gacaattcgc cccatgttca gtgcctgcac attaattctg
     4381 tcggcttcca gcatcaggga aagtttcatc gtttctccgg atgcttaccc agggtaatgt
     4441 tatttaccgt tctgtaattg tcgcgggtca tcaggccggt cgccctgcga gcccggagga
     4501 tatcaatact gtttattaac tgagagcggg gacaagtgct gaatcccggc tggtcggtac
     4561 gcaccagcgc gtatttttcc acgagaaaat tcaccgcatc acacagtgaa atgccagctt
     4621 caatatgctg ctcaatcaca cgttcatcgg cgaacggtgt gtcattcagt gtaaggccgt
     4681 aatgctggcc cagtagtcgg gagagcagag tctgccagat ggtgacggga gacgggcagc
     4741 tcgatgcctc ccgtacgtgt gtatcgggta atgttttcat actgaaggtt ttcctgatat
     4801 gcagagatga aaaatggaaa agtggcgtgg tgaaagcacc aggccggagc agaaggttat
     4861 tccggtggtt tactttttcg tttcgggcgt cggataaaca gccagataaa cgtaaccacc
     4921 gctaccgaga gtgtcagctt tgcaggtcag accttttgca tacagcgtga cggtatgctg
     4981 atggcgggga ttcagttcac cgctggtgag catgagttcc agttgtttca tcagcagcgg
     5041 aaaggcctgg tccaggtggt acgcatctgc atcgctgaac aggcctctga taccggcgcg
     5101 gtcggcaagg taatgcaggc ggttaccctc ctgcaccaga cgggcgccga aacagggcgt
     5161 cactgtgcag ggtagtcccc accagggatg gtcgtcattg tcatcgggat gcgttgtccc
     5221 ggaaagtgtg tctgacacga taaaatcctc acagaaaatc ggtgaatgat agtttaacga
     5281 tgacgggtca gccggcgcgc agaccggctt cggtaaaggt gtattcgttg atgagcagga
     5341 cctcatccac agcggcgtct gagctcagcc agtcgtactc gttttccggg tgacggaaca
     5401 tcagttcagg gtgacgctca tcagccggac atacggacca aaactgtcct tacggcgttc
//
LOCUS       DAZGLJ010000274         5334 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000274 DAZGLJ010000000
VERSION     DAZGLJ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.274"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..948
                     /locus_tag="ACHK49_004479"
     CDS             <1..948
                     /locus_tag="ACHK49_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO9264987.1"
                     /translation="SNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHKGI
                     VNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLESIL
                     GALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQLSQ
                     FQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLLPE
                     EQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWPLE
                     KLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1157..2122
                     /gene="lipA"
                     /locus_tag="ACHK49_004480"
     CDS             1157..2122
                     /gene="lipA"
                     /locus_tag="ACHK49_004480"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO9264988.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(2223..2426)
                     /gene="tatE"
                     /locus_tag="ACHK49_004481"
     CDS             complement(2223..2426)
                     /gene="tatE"
                     /locus_tag="ACHK49_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO9264989.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(2555..3343)
                     /locus_tag="ACHK49_004482"
     CDS             complement(2555..3343)
                     /locus_tag="ACHK49_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO9264990.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            3436..3819
                     /gene="crcB"
                     /locus_tag="ACHK49_004483"
     CDS             3436..3819
                     /gene="crcB"
                     /locus_tag="ACHK49_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO9264991.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3874..4083)
                     /gene="cspE"
                     /locus_tag="ACHK49_004484"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3874..4083)
                     /gene="cspE"
                     /locus_tag="ACHK49_004484"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO9264992.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(4258..4818)
                     /gene="pagP"
                     /locus_tag="ACHK49_004485"
     CDS             complement(4258..4818)
                     /gene="pagP"
                     /locus_tag="ACHK49_004485"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO9264993.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         1460 a         1319 c         1151 g         1404 t
ORIGIN      
        1 agtaataatc aaattgaacc ctgtttaagc agaaagtcgt cagaaggtaa accacaaata
       61 tttacaacct tacgaaatat tgatcttaac cttctgacta tttttgaagc tgtatatgta
      121 cataaaggga tcgttaatgc agcgaaagtg cttaatctga ccccctcggc aatcagtcag
      181 tctattcaga aactgcgcgt tatattccct gacccattgt ttattcgcaa aggccagggt
      241 gtcactccta ccgcatttgc gatgcatcta catgagtata tcagtcaggg ccttgagtcc
      301 attcttggcg cgctggatat cgaaggaagc tatgataagc aacgaacgat aactattgcg
      361 accactccct cggtcggagc cctggtcctt cctgtcatct atcgggcgat taaaactcac
      421 tatccgcagc ttttactgcg caacccgccc atcagcgacg cggaaaacca actcagtcag
      481 tttcaaaccg atctcatcat cgataacatg ttttgcacca atcgtacggt gcaacatcat
      541 gttctgttca ccgacaatat ggtgttaatt tgccgcgagg gaaatccact actctcttta
      601 gaagatgaca gagagactat cgacaacgct gcgcatgtac ttctgttacc ggaagagcaa
      661 aatttcagcg gtctgcggca gagagttcaa gaaatgtttc cggaccggca aatcaatttc
      721 accagctaca acattttgac aatcgctgca ctggttgcca acagtgacat gttagcgatt
      781 attcccagcc gtttttataa cctgtttagc cgctgctggc cgctggaaaa actgcctttt
      841 ccgtccttaa atgaggagca aatagatttc tctatccact acaacaaatt cagcctgcgt
      901 gatcccatcc tgcacggagt aatagatgtt atccgtaatg cattttgaaa aagtattgtt
      961 acagccagat cacagcgcag aacgataaaa tggcacaatc caacaacaat tttacatttt
     1021 cgcgaccgct ttggctgctt tcaggtccgt ttcaatgata tactgccagt cgttaattca
     1081 aaaatagttg ataattacaa caatctattg aattgaaacg ctttccttcg taattcgcaa
     1141 ctggaacacg cacgctatga gtaaacccat tgtgatggaa cgcggtgtta aataccgcga
     1201 tgccgataag atggccctta tcccggttaa aaacgtggca acagagcgcg aagccctgct
     1261 gcgcaagccg gaatggatga aaatcaagct tccagcggac tctacacgta tccagggcat
     1321 caaagccgca atgcgcaaaa atggcctgca ttctgtctgc gaggaagcct cctgccctaa
     1381 cctggcggaa tgcttcaacc acggcacagc aacgtttatg atcctcggcg ctatttgtac
     1441 ccgccgttgt ccgttctgtg atgttgccca cggtcgcccg gtagctcctg atgccaatga
     1501 accagtgaaa ctggcgcaga ccattgccga tatggcgctg cgttatgtgg ttatcacctc
     1561 cgttgaccgt gatgacctgc gcgatggcgg tgcccagcac tttgcggatt gcattactgc
     1621 cattcgggaa aaaagcccgc aaatcaaaat tgaaactctg gtgccggatt tccgtggtcg
     1681 tatggatcgt gctctggata ttctgactgc aacgccacca gatgtgttca accataacct
     1741 ggaaaacgta ccgcgtattt accgtcaggt acggcctggt gcagattaca actggtcgct
     1801 gaagctgctg gaacgcttta aagaagcgca tccggaaatc ccgaccaagt ctggtctgat
     1861 ggtgggactg ggtgaaacca atgaagaaat tattgaggta atgcgcgacc tgcgccgtca
     1921 tggtgtgacg atgttaacgc tggggcaata tttgcagcca agccgccatc acctgccggt
     1981 tcaacgttac gttagcccgg atgagttcga cgaaatgaaa gccgaagcgc tggcgatggg
     2041 ctttacccat gctgcatgcg gtccgtttgt ccgctcttct taccacgccg atttgcaggc
     2101 gaaagggatg gaagttaagt aatcctgtaa taattactta catctgtctg ataaaaaacc
     2161 cgctttttga agcgggtttt ttgtatcgaa caagatattg agggagcgtc ctgctcgcca
     2221 cgtcactctt tatgagagag cttttcagcc tgaagatcaa cgtctgcgcc ttttttcgcc
     2281 gcagcatcgt catcattcat cgccttcttg aaccctttaa tggccgctcc aaggtctccg
     2341 cccagcgtac gtaacttctt agtcccaaac agcagaacga ccagcgccgc aactactagc
     2401 agtttggtaa tactaatctc acccatagat accttcttga catataaccg gcaacagaac
     2461 gcgccttaaa aaccagagaa tattaacggt cattcgacgc gcgcacacaa tagcaatctg
     2521 taacaaggtg aatcaagcgc cgagtaaaaa aacatcataa taattgcggc ggcgcaaagc
     2581 gacggttgtt taagacgggc agttgcgcgc gcacctgacg cacacgttcg ggcgtcactt
     2641 ccgccataat gagtgcaggc atttctgacg ctgccgcaat ggtgacgcca aagggatcta
     2701 taatccggct ttgaccgata tttttgttcc cgcactcccc cgccgccacc atataacagg
     2761 tggtatccag cgcacgagcg gcaagcaacg ttgaccaatg atgctctttg agcggcccgc
     2821 gaacccaggc ggcaggaagt accaggattt cagctccctg taatgcctgt gccagcgcca
     2881 gctctggaaa gcgtaagtca taacaggtca tcagaccgac cttcatcccc tccacctcga
     2941 gtaacggagc gatttcatta ccagcatcaa cacggcgtga ttcctgaatg gcaaatgcat
     3001 catagagatg caatttggca taacgggcga cgatgtttcc tgcctgtagc gccaccagca
     3061 tattccatgc gcgccccggc gttgaaggaa catgaatcgt cagaattgtc gtcatcatgt
     3121 tacgtttact ttctcgccgt aaacgtccga ggaattcgcc ttccagcagc tgtgctgatt
     3181 taaccgatag atctgcatca tgatcatcgc gcgccagcaa tgcttccggc aggacgaaca
     3241 gcgatacgtc gttttccgcc gcctgcgcca tcaacgaggc acaaatctca gcgttctttt
     3301 cccacacaga tgtaacagca aactgtcctg cggcaaccag cattgccacc tcctgtcaga
     3361 gtcgtaaaat tcgccaacga cgattattcg gcgttacact tatcactcat acaaatcaaa
     3421 tagcaggatt ttgcagtgtt acaacttctt ttagcagttt ttattggcgg tggtacggga
     3481 agcgtggcga gatggctgtt aagtatgcga tttaacccgc tgcatcaggc gattccgttg
     3541 gggacgctgg cagcaaacct gattggggca ttcatcatag gaatgggatt cgcctggttc
     3601 agcaggatga cgaacattga tccagtgtgg aaagtattaa tcaccaccgg attttgtggc
     3661 ggtctaacaa ccttctcaac attttcggca gaagtggtgt ttttgttaca agagggccgc
     3721 tttggctggg cgttactgaa cgttttcgtc aaccttctgg ggtcttttgc catgacagca
     3781 ctggctttct ggctgttttc ggcctcaacc gcacactaaa gaccaaaaaa aaacccgctg
     3841 attaagcggg ttttgaattc ttgctgacgt atcttacaga gcgattacgt ttgcagcaga
     3901 agggcctttg gcaccgttag tgatttcgaa ctctacgcgc tgaccttcag caagagtttt
     3961 aaaaccatta gtctggattg cagagaagtg tacgaacacg tctttgctgc cgtcttccgg
     4021 agtaatgaaa ccgaatcctt tggactcatt aaaccactta acgttacctt taatcttaga
     4081 catcaaaatt acctttacat gaaaaataga cacaaatgct gtgtcggtta ccagtacacc
     4141 aattgtggta cgctttgtcc agtgcaactt tactaaaaag tgataaaagt cgcgttattt
     4201 ttgatgttac ttattttagc tattgatttt aaagaagtta ctaaaacttc atttgtctca
     4261 aaactgaaag cgcatccagg caaagtacac attgccattg ttgtaggtac ccggaatgta
     4321 ggtcatctga aaagtcactg ggccataacc cacggaggcc aatggcagta gaaccgggag
     4381 agggatgtaa ttccagttat cgcgtgccgt tacgccagcg gtgaatccca gacctaaatg
     4441 aaaattttca tccgccagcg gtcgccaggt actttcccat ccgtatccgg caatcggttc
     4501 ccatttgttc cacgagtcct taaatgccat ggcatacagg ccatgccagt ttcctttttc
     4561 atcccaacgc gacaggccaa aaccgccacc ccacggtcgc tcgttatagc gatcggtttt
     4621 ttctttgtcg taagcgaaac gtgcatgcca ggtgatggca ggaatatata aatcataatg
     4681 ttcaggctgt tgccaggttt gtgcaatatt ttctctaaac gttgtcatcc actcatctgc
     4741 gttagcaaaa actttaccaa cgctgattaa ctgaataaaa acaaaggaaa agatagcgac
     4801 atatttactc acgttcattt gtgaccataa aacatttatc aaaaatctac tactagcata
     4861 gcaaagctac aattaacata accttaatag acccaacata aagaataatc tgaatagctg
     4921 ctctatttat ttccgcttgt tcaagttaaa tatttccgcg catcgattaa agatgatgcg
     4981 tcgttttctg cgatgggaat agtcaaaaaa gaaaaaccaa gtcttttttg atgacaaaat
     5041 gcaatcaagg aaaaattatt ttatttttta actagataca acaatcatct taacgaagta
     5101 tataatataa atcgtcaacc catgctcatg ttagattatt ttttgagcgc acagccgcag
     5161 ctaacaccat tgcaattaac aaatttgcat caatccacca tcaatttaca cacattatta
     5221 tgtgataatt gccaaccgct aaatatgcgt tttgttatct atgtataaaa acagcaactt
     5281 caatgtctta atggcagttt ttcttgattt taatcagcat tcatcgccaa ttta
//
LOCUS       DAZGLJ010000275         5319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000275 DAZGLJ010000000
VERSION     DAZGLJ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.275"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..264
                     /locus_tag="ACHK49_004486"
     CDS             <1..264
                     /locus_tag="ACHK49_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9264994.1"
                     /translation="YLALSNHARLFGFTAEDIMDFWQHKAPQKYSAFELAFELGHRVI
                     AELILNTLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(233..1969)
                     /gene="aceK"
                     /locus_tag="ACHK49_004487"
     CDS             complement(233..1969)
                     /gene="aceK"
                     /locus_tag="ACHK49_004487"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO9264995.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPHFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2250..3554)
                     /gene="aceA"
                     /locus_tag="ACHK49_004488"
     CDS             complement(2250..3554)
                     /gene="aceA"
                     /locus_tag="ACHK49_004488"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO9264996.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(3584..5185)
                     /gene="aceB"
                     /locus_tag="ACHK49_004489"
     CDS             complement(3584..5185)
                     /gene="aceB"
                     /locus_tag="ACHK49_004489"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO9264997.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
BASE COUNT         1245 a         1429 c         1353 g         1292 t
ORIGIN      
        1 tatcttgctt tatctaacca tgcacgcctg tttggattta ccgctgaaga tattatggat
       61 ttttggcaac acaaagcccc acaaaaatac tctgcctttg agttggcttt tgaattgggt
      121 caccgggtta ttgctgaatt aatccttaat acattaaata agatggctga aagctttggc
      181 tttacggata accctcgata cattgcggag aaaaattata tggaagcttt actcaaaaaa
      241 gcatctcccc ataccgtacg ctaaatcttt gcctgcgccg atacgcataa acatcttcca
      301 catgcccttc acgtatgcgg ttttgtagtg cgcgccagta atcagcgcgg aacaggtcgg
      361 cgtgcatctc ttcaaacagc ggaccaatac gcgggtcggc gcatagccag tggcgaaact
      421 cttccgggaa aacatcgccc ggcgagacgc tgtaccacgg ttcgctggca agttcgtctt
      481 ccggatagcg cggcggcggg atgtcgcgga aattcacttc cgtcatgtag caaatttcat
      541 cgtaatcata aaaaaccaca cgcccgtgac gggtgacacc gaagttttta aacagcatgt
      601 cgccaggaaa aatgttagca gcggcaagct ggcgaatagc gttaccgtat tcttcaatgg
      661 cgtcgcgcaa ctgctgacct tccacttgtt ccagccagat attgagcggc accatccgcc
      721 gctcgatata gagatggcga attacaatct gctcaccaag atcggtgatt ttttccgctg
      781 cttcctggag cagtaattcc attaatgccg gggaaatatg ccgcttctcc agcacaaagt
      841 tttcaaactc ctgggtgtcc gccattcggc ccacacggtc gtgctctttc accagttgat
      901 agcaggcacg aacgtgagcg gcagacatct ctttctgcgg cgcgaacttg tctttgatga
      961 ctttgaatac ccgatcaaag cccggcagcg taaataccaa catcaccatt ccacgaatac
     1021 ccggcgcttc aatgaactgc tcattacagc cctgtagata aacgagatat tcgcggtagc
     1081 tttcggtttt ggcgtgcttc tggcagccga tagccatata caattcagca gtggttttac
     1141 ccggcagaat ttcccgtagc cactcgacca gcgctgcggg cagcggtgca taaaccataa
     1201 aataagaacg cgcaaagcca aaaacaatgc tcgcttcggc ggtcgtcgtc aggcaggtat
     1261 caataaataa ctcgccgtcg tccgtctggt ggatcggcag taagaagggc aacgtgccgg
     1321 aaggcgtgat cagtttgccc accagccaag cggctttatt gcggtaaaac agttcgttcg
     1381 ccacctgtaa atgactttcc gcgaggttgt ctgtccccag cgtttccgtc agatggcgaa
     1441 tgatgtaatg aatgtcacgg cttttattct gccagcgcag gcgcagcggc aggtcgctga
     1501 taacgcgcat cagtagagat tcccagccgt gatcggggtg aaagtctttc gccagcgggc
     1561 ggggaatggt acgaaagcgg cgctctggct gagagctaaa gataaaaagc cgctcgggag
     1621 taagcgagcg gtggtcaaat aaccgacagt acacggagtt aaaaaagctc tccgcaatct
     1681 cgaagtgcgg gtaatccggc aacagccgcg tgtaatgctc tttaacgcgc agtaaaaatg
     1741 ccgcgtccgt actttggccg ttagtaatgc agcgcagttg ctccacgacc agaccaacgt
     1801 gatgatcgta aagatggata cggtttttca tcgcctgctg gacagcatgc cagtcggcct
     1861 gttcgaaacg ctgctgcgcc ccggaagtca cttcgaggaa tcgaccatac tgagcgtcga
     1921 agccttgcaa aatggtttga gcaatcagta attccaggcc acgcggcatt tagcgccctc
     1981 atcaggagca gagaattgcc ggatgcggcg taaacgcctt atccagccta cgttcggcaa
     2041 cggttgtagg catgataaga cgcgcaagcg tcgcatcagg catcgtgcac cgattgccgg
     2101 atgcggcgta aatgccttat ccggcctacg ttcggcaacg gctgtaggca tgataagacg
     2161 cgcaagcgtc gcatcaggca tcgtgcaccg attgccggat gcggcgtgaa cgccttatcc
     2221 ggcctacagt cagcaacggt tgttgttgct tagaactgcg attcttcagt ggagccggtc
     2281 agcgcggtga ctgaagacgt gccgccctga ataatagtcg tcactttatc gaagtaacct
     2341 gtacccactt cctgctggtg agatacgaag gtatagccat ctttcgcggc ggcaaattcc
     2401 ggctgctgca ctttctcaac gtagtgcttc ataccctcgc cctgggcata ggcgtttgcc
     2461 aggtcaaaca tgttgaacca catgctgtgg atacctgcca gggtgatgaa ctggaacttg
     2521 tagcccatat ccgacagctg ctgctggaag ctggcaatag ttttgtcgtc gaggtttttc
     2581 tgccagttga acgacggcga gcagttataa gccagcagtt tgcccggata tttcgcgtgg
     2641 atagcttgtg caaagcgacg cgccagttcc agatccggcg tggaggtttc acaccagacc
     2701 aggtcagcat atggcgcata cgccaggcca cggctgatcg cttgctcaat gcccgcatga
     2761 gtacggaaga agccttcact ggtacgctcg ccagtaataa attcactgtc atacgggtcg
     2821 caatcggagg taatcagatc cgccgcatcg gcatcggtac gagcaaccag cagggttgga
     2881 acgcccgtca cgtcagctgc aagacgcgcc gcgaccagtt tctgaatagc ttcctgagtt
     2941 ggcaccaaaa ctttgccgcc catgtgaccg catttcttca ctgacgccag ctgatcttcg
     3001 aagtgaactg ccgctgcacc ggcttcaatc atcgctttca tcagttcaaa ggcattcagg
     3061 acaccgccaa aaccggcttc cgcatcggca acgatcggca ggaagtaatc gacatagcgc
     3121 ggatcgcccg gctcaatgcc tgcggaccat tggatctgat cggcacgacg gaaggtgttg
     3181 ttgatccgct ccaccacagc tggcaccgag tttgccggat agagcgactg atccggatac
     3241 atgctggccg ccaggttagc gtccgccgct acctgccatc ccgacagata gactgcttca
     3301 atacccgctt tcgcctgttg cagcgcctga ccgccagtca gtgcaccgag gctgttgatg
     3361 tagccttttt tcgactcgcc atgcagcaga cgccacattt tcgccgcgcc cagttgcgcc
     3421 agggtgcatt caggattgac tgaaccgcgt aatttcacca catcttccgc gctgtatggg
     3481 cgagtaatgc cttcccaacg cggttgagtc cactctttct gtaattcttc aatttgttgt
     3541 gtacgggttt tcatgtgcag atgctccata gttatgtggt ggtttacgct aacaggcggt
     3601 agcctggcag ggtcaggaaa tcaattaact catcggaagt ggtgatctgt tccatcaagc
     3661 gtgcggcatc gtcaaaacgc ccctgggaga aacgttcttc gcccagttcg ctggcaatga
     3721 ctttcatctc ttcacccagc atctggcgga acaaggcttt ggtcaccggt ttgccattgc
     3781 tcaacgtttt ttgatgatgg atccactgcc agattgaggt acgggaaatt tcagccgtcg
     3841 ccgcatcttc catcaggcca taaatcggca cgcagccgtt gccggagatc catgcttcga
     3901 tgtactgcac tgccacgcga atgttggcgc gcataccttc ttcggtgcgt tcaccatcac
     3961 aaggtgccag cagctgatcg gcagtaatcg gcgcgtcttg ttcgcgcatc acttcaagct
     4021 gatttttacg ggagccgaga atgtcgttga ataccgccat tgccgtatcc gcaaggcctg
     4081 ggtgagcaat ccatgtgcca tcgtgaccgt tattggcttc cagcgcttta tccgctttta
     4141 ctttgttgag cacctggtta ttgcgctctt catctttgct cggaataaac gctgccatgc
     4201 cgcccatcgc aaaagcaccg cgtttatggc aggttttaat caacaggcgt gagtaagcat
     4261 tcaggaatgg tttatccatc gtcactgcct gtctgtctgg caggacgcga tcgggatagt
     4321 ttttcaacgt tttgatatag ctgaagatgt aatcccaacg accgcagttc agaccaacaa
     4381 tatggtcacg cagcgcgtga aggatttcat ccatctggaa cacggcgggc agcgtttcaa
     4441 tcagcaacgt cgccttgatg gtgccgcgcg gcagattaaa gcgatcttct gcatagctga
     4501 agacttcgct ccaccaggcc gcttcctgcc aggactgggt tttcggcaga tagaaatagg
     4561 gaccgctgcc ctttgccaac agtgcctgat agttgtggaa gaaatagagc gcaaaatcaa
     4621 acaggctacc ggggattgcc tcaccacgcc aggtgacatg tttttccggc aagtgcagac
     4681 cgcgtacccg acaaatcaaa accgctggat tgggcttgag ctgataaatt ttgcctgctt
     4741 cattggtata gctgatggtg ccgttaaccg catcgcgcag gttaatttgc ccgtcgatca
     4801 ctttgttcca gtctggtgcc agtgaatctt cgaaatcggc cataaagact ttcacattgg
     4861 cgttcagtgc gttgatcacc atcttgcgct caaccgggcc agttatctcg actcgacgat
     4921 cttgtaagtc cgcgggaata ccacgaattt tccagtccgt atcgcgaatg gaagctgttt
     4981 ccgaaataaa atcaggcaaa gttccgttat caatatcttg ctgctgctga atgcgcgctg
     5041 ccagaagttt attgcgttgt ggcgtaaaat gcgtcaccag ctcagtcaga aattctaccg
     5101 cttcggcagt aagaatttgc ttctcctgct cgccatacgg ccttatgaaa gccagttcat
     5161 cggttgttgt tgcctgttca gtcatcgtgc agctcctcgt catggacccg aaaacttccc
     5221 cactgtgaac gaaggatcgt tgtgcgcttt tcgttcagca caactaagac tactcattta
     5281 aaatgcaaaa tcaaaaacaa tttccatttt aattaaaaa
//
LOCUS       DAZGLJ010000276         5306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000276 DAZGLJ010000000
VERSION     DAZGLJ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.276"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            52..471
                     /locus_tag="ACHK49_004490"
     CDS             52..471
                     /locus_tag="ACHK49_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFO9264998.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            468..1028
                     /locus_tag="ACHK49_004491"
     CDS             468..1028
                     /locus_tag="ACHK49_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFO9264999.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDTSSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            1029..1274
                     /locus_tag="ACHK49_004492"
     CDS             1029..1274
                     /locus_tag="ACHK49_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO9265000.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRPLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            1271..2773
                     /locus_tag="ACHK49_004493"
     CDS             1271..2773
                     /locus_tag="ACHK49_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO9265001.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDVPVRITRASQATELWGRGSMIALMVAEFIAINPDAELYAIAQGNGT
                     GQATAAAMNLTGTVTRDGIVYACVGGRRYTLPAPKGKKGKELTDELAALINADPDAPF
                     TASSGAGSGDNGAGLKGSLGITARFTGECSVHDVRLNYHDGEATPEGIQVAIAYPKQK
                     AANPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWLAHTG
                     TFGEVQAFGAKRNDFLATCTSIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALP
                     SRMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQIERQVTMYRQNVYGESDPSYLD
                     VETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWV
                     ELGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            2782..3147
                     /locus_tag="ACHK49_004494"
     CDS             2782..3147
                     /locus_tag="ACHK49_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO9265002.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASASSTRVQ"
     gene            3162..3638
                     /locus_tag="ACHK49_004495"
     CDS             3162..3638
                     /locus_tag="ACHK49_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO9265003.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            3765..>5306
                     /locus_tag="ACHK49_004496"
     CDS             3765..>5306
                     /locus_tag="ACHK49_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO9265004.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSIQAADAN
                     ILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGAQKDAMN
                     SWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDGSYHSLAESTAS
                     AMNQGFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATRALKMARAVGTGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPGTSQGRGQRFI
                     NFLTGVNPAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRGPGRGRPVTPPLP
                     PSPVPP"
BASE COUNT         1186 a         1394 c         1628 g         1098 t
ORIGIN      
        1 acaggatgct ccccggaaaa agaccggtaa caaagcggaa caggcccgtg catgaattac
       61 gccactgaaa ccgatatgcg ggcgcgttac cgcgaggatt tgctcaggcc gttactggct
      121 gtgccccgtt cggatgagcc ggacacgcgc aagctgaacc gggcgctgac agatgcgtca
      181 gcccttatcg acagctatct gtccgcccgt tacacgttgc cgctggaggt tattccggcc
      241 gttcttgttc agcactgttg cgcgattgcc ttttattacc tgtgcgatca gcgagcctcc
      301 gatcaggcgc gtgaccgtta ccgtgaagcg ctggcctggc tgaaagatgt catgaacggc
      361 aatgtgccgg tcggtgtgga tacgaacggt gcggcccctg aatccgggga tttaccacag
      421 gttcagtctg atgcagcggt attcgggcgc aaccagaagg gcttcatatg attacggaaa
      481 ccgaacaggc atacatcgcc cgtatccgtg agtatttcgg gaatgaactg gtgtctgttg
      541 acacgcatcc cggcgactgg agcgacagcg tactgcgcac catgctgatt aacgccccgg
      601 cgatctacgt tgcctggctg ggtgccggtg aaggccgtac ccgtggtcgc ctggtcagtc
      661 actgggtgtt ctacgtcatc ggcgacatgc tcaacgggcg tgaggccagc cgtcccggac
      721 tgtatcagat tgtggcccgg ctgattgccg tgcttaacgg cttcagaaca gaaaaaacct
      781 caccgcttta ctttgaaaag gcggtcaacg gttacaccga aacccaggcg gacagtggtg
      841 cggtgatgta tgcgctttat ttctcctgcg aggaaatgat cgcaccgctg accgatacca
      901 gctcgctgga cgacttcctg cgtcattacg aaacctttgt ggagccgccc ggtacgccgc
      961 cgtttgaggc acatattgat ttgccgggga acacgacaac ggcggtcgaa ccgccggagg
     1021 aactgtaaat gaaaaccatc tttatcaaac ccgcgccggg acgcctgatt cgtgatcccg
     1081 acacaatgcg cccgctggca caggaggggg aggaaaagcc ctttacgccg ttctggtgtc
     1141 ggcgtcttga tgacggcgat gtcattcagg ctgaaaaagc cgctgaagag gctccggctg
     1201 tatctgcgga tgcaacgact gcgacagaaa aaccggtcgc ccaaccggca tccgataagg
     1261 agaaaccgca atgattaatt ttgacgggat cggtaatgac cgccgtatac cgctgattga
     1321 ggttgagttt aataactcca tggctgtcac cggcactccg gcacagcgtc agtgtgtgct
     1381 gctgtttggt caggcgagaa tgaaagagaa tgccgtggac ggtgccggtg ttctggatgt
     1441 tccggtcagg atcacccgtg cgtctcaggc aacagaactc tgggggcgcg gctccatgat
     1501 tgccctgatg gtggcggaat ttatcgccat caatcctgat gctgaactgt atgccattgc
     1561 tcaggggaac ggcaccggac aggcaacggc tgcggccatg aatctgaccg gaacggtcac
     1621 ccgtgacggg attgtttatg cctgcgtggg ggggcggcgt tatacactcc cggcaccaaa
     1681 aggtaagaaa ggcaaagaac tgaccgacga gctggccgcg ctgatcaatg ccgatcctga
     1741 cgcgcccttc acggcctcct ctggtgcagg aagtggtgat aatggcgcag gtctgaaagg
     1801 gtctctgggg atcaccgccc gctttaccgg tgaatgctcc gttcatgatg tgcgcctgaa
     1861 ctatcacgac ggggaagcaa ccccggaagg aatacaggtc gctatagcct atccgaaaca
     1921 aaaagcggct aacccggata tcacccgaag cgtggcgggt atgggcgatc gccagtacaa
     1981 ctatgtcgtc atgccctata aggatgatgc aaacctgaaa atcatcagtg atgagcttct
     2041 gaaacgctgg ggaccggcca aaatgtctga cggcgtgctg tggctggcac ataccggcac
     2101 gtttggtgag gtgcaggcat tcggggcaaa acgcaatgat tttctggcga cctgcacatc
     2161 cattcccaaa gcccctgaac cggattacct ctgggcagcg gcggtctgtg ccacctgtgc
     2221 accctctctg ggagcagatc ctgcgcgtcc gcttcagacg ctggcgcttc cgtcacgcat
     2281 ggccccggct ccggccgatc gtctgacgcg tgaagagcgt aacagtctgc tgtggggagg
     2341 gattgccacg gtcagtgtgg ctgccggtga tgtggttcag atcgaacgtc aggtgaccat
     2401 gtatcgccag aacgtgtacg gcgaaagtga tcccagctat ctggatgtgg aaaccatcta
     2461 caccctctcc tggctgcgtt attccctgcg tacttttatc acacaacgtt ttccgcgcca
     2521 taaactggct gatgacggga cgcccgttcg tgccggtcag aatatcgtga cgccggagat
     2581 catgaagctt cagttaattt cgctgggtga agagtgggtg gaactcgggc tggtcgaaaa
     2641 tctggacacc tttaaaaaga atctgcttgt ggagcgtaat gcgtcagatc gaaaccgtct
     2701 tgacgtgtta tgcacgccgg atctggttaa ccagttccgc ttcctggcag cacagatccg
     2761 tttcattttg tgaggtaagg catgagcgga aaacagtatc agggtacggc cactatccgt
     2821 gtgaacggtc aggaatacga gacgcttgag ggggctacat tttctccgtc tggttttgaa
     2881 cgtgaagtgg tgaaaggcgc aaaagtctat ggttaccgcc agaaaccacg tgaagcgacg
     2941 ctggactgca aatttccggc aggcggagaa ggttcaccag ccgccgatga aatcaacaac
     3001 tggactgcgg tcactatcga gtttgtggcg gataccggcg aagtccacat gatgacgaag
     3061 gcctggagca gtgaaccggc ctcgcttgac ggtggcgggg agatctccgt gaagttcgcc
     3121 agcgcctcca gtacccgtgt tcagtgatca ggaaaaataa aatgaccaca cgtaagaaaa
     3181 aaacggcagt ttccgaggca gccgtgatgg aggcaatccg ggaagcactt gagggggctg
     3241 atccacgcac tgccgggctg acagagcaac tggcaaaggg atatgtggat ctgcttgatg
     3301 gtctgccgtt tggtgagacc cgtgaatatc gtgtcacgtt ccgggaactg acagcgaaag
     3361 acagtattga cgcagaagca gaagccgagc gcgtggtgga gacaaacaat ggcccgatgc
     3421 tgatagcgtc tccgtcgtta cgcggtgttg cactgctgcg ccgtcagatt gccgcagtgg
     3481 gagacattga aggtccgttg tcaccccgtc agattggaca gttaagcgag cgcgatctct
     3541 cccgcctgat ggcagcggtc agcctgctgg ataccgcgct tgccggaaag ctggcagctg
     3601 accgggggcg atcaggcgca gtgtcgggat cagattgaag aagcggcaat tatcctgggg
     3661 atggtgacaa aaagcggtcc ggaatgggcg cttaacctcc ccctgtcgca actttaccgg
     3721 cactgccgac agacagaaaa aatcatcaga acgaagcagt aagtatggcc cgaaatctca
     3781 gagcatctct gatagttgat ttgctcggca atatctcagc caaatcccgc cagtggtcac
     3841 aggagctggg ggcattctca cgctccggcc aggcggggct gggtggcctg ggaaatgctg
     3901 cccgccgtgc cgggcaggaa acagatgtac tcggaagcag gatgcagcgc acgctggccg
     3961 gggtgcgggg cagtatccgc catgtcactt ctgattttga ccgtcttcag ggcagtatta
     4021 ccggcaccat cgggcggatc agcaatcttt acggaatgct ggccggtggt gctgctgtat
     4081 atggctttaa ccggggattt attcgcccgg cagcggagat ggaaacctat atgattcgcc
     4141 tgaactccct ttacaaaggg gatcgggcga aaactgatga tgtccgcagg tgggcaatac
     4201 agaatgcgaa ggaaaccacc tgggggctgg ctggcgtcat gcaggaatat acgtccagtc
     4261 tgggctttgg tatgagtgac agggaggccc gaaattttgt caccatgctt caggatcagg
     4321 gtgccgtggg cggctggtca ctgtccgatg cacagggggc atccctgcaa ctgaaacaga
     4381 tgtatgcaag aggcagtatt caggcggcgg atgcaaatat tcttgccacc tacggcatta
     4441 atgcctaccg cgtgctggct gatcgtctgg gtgtggacca gaaagtggtc agaaaacttg
     4501 gtgaaaaagg gctgcttggg ccggacagta ttcgcctgtt atttcagacg ctggcggaac
     4561 aggccagggg agcacaaaag gacgcaatga attcctggag tggcctgacg gccatgatgg
     4621 gtgacgtctg ggatcagttt gcccgggatg tgatggacag tgggccattt gaaaaactga
     4681 agggtaatct caggggattt ctttcatggg ttgacagtgc caaatctgat ggcagttacc
     4741 attccctggc ggaaagcacg gcatcagcca tgaaccaggg ctttgagtat gcccgtgatg
     4801 cagtgacagg gttttatcag gcgatcagaa aggtacgcga cacgcttcag gctctgcggg
     4861 atgcgggcta tggtgatgcg ctggatcgca ttggtcaggg ggcacagacc gccgcgaaat
     4921 acctgctgta catgtatctc gccacccgtg ccctgaaaat ggcgcgggct gtcggaacgg
     4981 ggattatacg tccgggtgct gcattgctgg gatacggatt atcagcggcg gccttcctga
     5041 cttcaccttt ccggcgctct cctccggccg gaacaccacc aggcacatct cagggacgag
     5101 ggcaacgttt tataaacttt cttaccgggg taaatccggc agccgttcag ccagtacttg
     5161 taacaaactg gcccacagga ggactgtccg gcaccggcgc gaccgatact tcctcaggcg
     5221 gacgtcaggg gcggggccgc aggaaacgcg gaccggggcg cggaagaccg gtcacgcctc
     5281 ccctgccacc gtccccagtg ccaccg
//
LOCUS       DAZGLJ010000277         5271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000277 DAZGLJ010000000
VERSION     DAZGLJ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.277"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..2294
                     /locus_tag="ACHK49_004497"
     CDS             <1..2294
                     /locus_tag="ACHK49_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO9265005.1"
                     /translation="HVFEEMTVYAPVPVPVNGNTHYTSESIERLPTGNGNISDLLRTN
                     PAVRMDSTQSTSLNQGDIRPEKISIHGASPYQNAYLIDGISATNNLNPANESDASSAT
                     NISGMSQGYYLDVSLLDNVTLYDSVVPVEFGRFNGGVIDAKIKRFNADDSKVKLGYRT
                     TRSDWLTSHIDENNKSAFNQGSSGSTYYSPDFKKNFYTLSFNQELADNFGVTAGLSRR
                     QSDITRADYVSNDGIVAGRAQYKNVIDTALSKFTWFASDRFTHDLTLKYTGSSRDYNT
                     STFPQSDREMGNKSYGLAWDMDTQLAWAKLRTTVGWDHISDYTRHDHDIWYTELSCTY
                     GDITGRCTRGGLGHISQAVDNYTFKTRLDWQKFAVGDVSHQPYFGAEYIYSDAWTERH
                     NQSESYVINAAGKKTNHTIYHKGKGSLGIDNYTLYMADHISWRNVSLMPGVRYDYDNY
                     LSNHNISPRFMTEWDIFADQTSMITAGYNRYYGGNILDMGLRDIRNSWTESVSGNKTL
                     TRYQDLKTPYNDELAMGLQQKIGKNVIARANYVYREAHDQISKSSRTDSATKTTITEY
                     NNDGKTKTHSFNLSFELAEPLHISQVDINPQIVFSYIKSKGNLSLNNGYEESNTGDNQ
                     VVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPSGLVWANTLAWQEARKARIILGKT
                     NVQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQFLKQQNLTISADILNVLDSKTAVD
                     TTNTGVATYASGRTFWLDVSMKF"
     gene            2351..5134
                     /locus_tag="ACHK49_004498"
     CDS             2351..5134
                     /locus_tag="ACHK49_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO9265006.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVHDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         1533 a         1188 c         1226 g         1324 t
ORIGIN      
        1 tgcatgtttt tgaagagatg acggtctatg ctcctgtccc tgtacccgta aacggcaaca
       61 cgcattacac cagtgaaagc atcgagcgtt taccgaccgg gaatggcaat atcagcgatc
      121 tgctgagaac caaccctgcg gtacgcatgg attcaacgca aagtacctcg ttgaaccagg
      181 gagatattcg cccggagaaa atctctattc acggtgcgtc gccctatcag aatgcctatt
      241 tgattgacgg tattagtgcc actaataact tgaacccagc gaatgagtcc gatgccagta
      301 gtgcaaccaa tattagcggg atgtcacagg ggtattatct tgatgtcagc ttactggaca
      361 atgtgacgct ttatgacagt gttgtgccgg ttgaatttgg tcgcttcaat ggcggggtaa
      421 ttgatgcaaa gatcaaacgc ttcaacgctg atgatagcaa ggtgaaattg ggttatcgca
      481 ctacgcgttc ggactggtta acatcgcata tcgatgagaa taacaagagc gcatttaatc
      541 aaggctcttc aggaagtact tattactccc cagattttaa aaagaacttt tataccttgt
      601 cgtttaatca ggaactcgct gataacttcg gcgttaccgc cggtttatcg cgccgccagt
      661 ctgatatcac ccgcgcggat tatgtttcga atgacggcat tgtcgccggt cgggcacagt
      721 ataaaaacgt tatcgatact gcattgagca aatttacctg gtttgccagc gaccgcttta
      781 cccacgattt aaccttaaaa tataccggct ccagccgtga ttataatacc agcaccttcc
      841 cgcagtctga tcgcgaaatg ggtaataaat cctacggtct ggcatgggat atggatacgc
      901 agctcgcatg ggccaaactg cgtaccaccg ttggttggga tcatattagt gattataccc
      961 gtcacgatca tgacatctgg tacaccgaac tttcatgtac atatggtgat attacagggc
     1021 gttgtacccg tggcggatta ggacacattt cccaggctgt agataattac accttcaaaa
     1081 cacgcctgga ctggcaaaaa ttcgccgtgg gtgatgtttc gcatcaaccc tacttcggcg
     1141 cggaatacat ctattccgat gcatggactg aacgccataa ccagtctgaa tcctatgtga
     1201 ttaatgctgc aggaaagaaa actaaccata ccatttacca taaaggtaaa ggcagcctgg
     1261 gaattgacaa ctacacgctg tatatggcgg atcacattag ctggcggaat gtgtcgttaa
     1321 tgcccggtgt gcgttatgac tatgacaact atctgtcaaa ccacaatatc tccccgcgct
     1381 ttatgacgga atgggatatt tttgctgatc aaacctcaat gattacagca ggttataacc
     1441 gttactatgg cgggaatatt cttgatatgg gattacgtga tatccgcaat agctggacgg
     1501 aatcggtatc aggcaataaa accctgacgc gttatcagga tttgaaaacg ccttataacg
     1561 atgaactggc aatgggattg cagcagaaaa tcggtaagaa cgttattgcg cgcgcaaact
     1621 atgtttaccg tgaagcgcat gatcaaatca gcaaaagcag tcgtaccgac agcgcgacta
     1681 aaaccaccat tactgaatat aacaacgacg gcaaaacaaa aacgcattcg ttcaacctca
     1741 gttttgaact ggccgaaccc ctgcatatca gccaggtaga tattaacccg caaattgtct
     1801 ttagctatat caagagcaag ggcaacttgt cgttaaacaa tggttatgag gagagcaata
     1861 ccggtgataa ccaggtggtt tataacggta atctggtttc ttacgatagc gttccagtgg
     1921 cagattttaa taacccatta aagatctcct taaacatgga tttcacgcat caaccgagcg
     1981 ggttagtgtg ggcgaatacg ctggcctggc aagaagcgcg taaagctcgc attatcctgg
     2041 gtaagacaaa tgtgcaatac atcagcgaat attcagatta caagcagtat gttgacgaaa
     2101 aactggatag cagcctgacc tgggacaccc gcttgtcctg gacgccacaa tttctgaaac
     2161 aacaaaacct gacgatcagt gccgatattc tcaatgtatt ggatagcaaa accgctgttg
     2221 atacaacgaa taccggtgtg gcgacctacg ccagtggccg tactttctgg cttgatgtca
     2281 gcatgaaatt ttaatagttc ggattttact taaccgggta aattcgcccg gttttcgcat
     2341 ggagataatt atgagaaacc tctgtttctt actgacgtta gtggcaactc tgttgctccc
     2401 cgggcggctg attgccgccg ccttaccgca ggatgaaaag ttaattaccg ggcaactggg
     2461 caatggcttg cgatatatga tttatccgca tgctcatcca aaagatcagg taaatttatg
     2521 gctgcaaatt cataccggtt cattgcagga agaagacaat gagcgcggcg tggctcattt
     2581 tgtagaacat atgatgttta acggcacaaa aacatggccg ggtaataaag tcatcgaaac
     2641 atttgagtca atgggcctgc gttttggtcg cgatgttaat gcctatacca gctatgacga
     2701 aacggtgtat caggtgagtt tgccgactac gcagaaacaa aatctgcaac aagtgatggc
     2761 aatcttcagt gaatggagta atgccgcaac ctttgaaaaa ctcgaagtag acgctgaacg
     2821 tggcgtaatt actgaggaat ggcgtgccca tcaggatgcg aaatggcgca cctctcaggc
     2881 gcgccgccct ttcctgctgg ctaatacccg taatttagac cgtgaaccta tcggcctgat
     2941 ggatactgtc gccacggtca caccggcaca attgcgccaa ttttatcaac gctggtatca
     3001 accaaataat atgaccttta tcgtggttgg cgatatcgac agtaaagaag cgctggcgct
     3061 gataaaggat aatttaagta agcttccggc taacaaagca gctgaaaatc gcgtctggcc
     3121 gacaaaagcc gaaaaccacc tgcgctttaa tatcattaat gataaagaaa accgggtgaa
     3181 cggcatcgca ctctattatc gcctgccaat ggtacaagtg cacgatgagc aaagctttat
     3241 cgaacaagct gaatggagca tgttagttca gctgttcaat caacgtctgc aggaacgcat
     3301 acagtcgggc gagttgaaga ctatttctgg cggcactgcg cgcagcgtta aaattgcacc
     3361 cgattatcag tcgctgtttt tccgtgtaaa tgcacgagac gataatatgc aggatgctgc
     3421 gaatgcatta atggcagagt tggcaactat tgatcagcat ggtttttctg ctgaagaact
     3481 cgatgatgtt aaatctaccc gcctcacctg gctgaaaaat gcggttgatc agcaagctga
     3541 acgtgattta cgtatgctga ccagtcgcct ggcatccagc tcattaaata atacgccgtt
     3601 cttgtcgccg gaagagacat atcaactttc gaaacgtctg tggcagcaaa ttaccgtgca
     3661 aagtctggcg gaaaaatggc agcagttaag aaagaaccag gacgcatttt gggagcaaat
     3721 ggtaaacaat gagcttgccg ccaaaaaagc attgtctcct gcggctatcc tggcgctgga
     3781 aaaagagtac gccaacaaaa agctggcggc ttacatcttc ccaggcagaa atttatcgtt
     3841 aacagtagac gctgacccac aggcggaaat tagcagcaaa gaaacgctgg ctgagaatct
     3901 gacatcatta acactttcca atggtgccag ggttattctg gcaaaatccg cgggtgaaga
     3961 gcaaaagcta caaattactg ccgtatcgaa taaaggcgat ttaagtttcc ctgcgcagca
     4021 aaaatcactt atcgcgctgg caaataaagc agtaagcgga agcggcgttg gtgagctctc
     4081 ctcttccagc ctgaaacgct ggagtgcgga aaattcggta accatgagca gtaaagtcag
     4141 tggcatgaat acgttgctct ccgttagcgc gcggactaat aaccctgaac ctggttttca
     4201 gttgattaac cagcgaatca cccacagcac gattaacgac aatatttggg catcgctaca
     4261 aaatgctcaa attcaggcgt tgaaaacgct cgaccagcgt ccagcggaga aattcgccca
     4321 gcagatgtat gagacgcgct atgctgatga ccgcacgaaa ttactgcaag aaaatcagat
     4381 tgtacagttt actgccgcag atgcgctggc tgccgatcgc cagttgtttt catctccagc
     4441 ggatatcacg tttgtcattg tcggtaatgt ctcagaagac aaactcgtgg cgttaattac
     4501 gcgttactta ggatcaatca aacactctga ttcgccatta gccgcaggta aaccattaac
     4561 tcgcgcgacg gacaacgcat cggttactgt aaaagaacaa aatgaacctg tggcacaggt
     4621 ttcacagtgg aagcgttatg attcccggac acctgttaat ctggcgacgc gtatggcgct
     4681 cgatgctttt aacgtcgcac tggcaaaaga tctacgtgtt aatattcgtg aacaggcatc
     4741 tggagcatac agcgtttctt ctcgcctctc ggttgatcct caggccaaag atatcagtca
     4801 tttgctggct tttacttgtc aaccagaacg acatgatgaa ctgttaacgt tagcgaatga
     4861 agtgatggtt aagcgtctgg ctaaagggat cagtgagcaa gaactgaatg aataccagca
     4921 aaacgttcag cgcagcctcg atatccaaca gcgtagcgtt caacaattag cgaacactat
     4981 tgtaaatagt cttattcaat atgacgatcc tgcagcatgg actgagcagg agcaattgtt
     5041 gaaacaaatg acggtagaga atgttaacac tgccgttaaa caatatcttt ctcatccggt
     5101 aaatacttat accggagtat tattgccaaa ataataacag ccccgtcaac acatcgttgg
     5161 cggggatttt agcaatattc gctattttta tgtaataatt ttataaatgc gttcaaaata
     5221 ataatcaagt actaatagtg atattttaag gtctgatttt tacgtgataa t
//
LOCUS       DAZGLJ010000278         5266 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000278 DAZGLJ010000000
VERSION     DAZGLJ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.278"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..934)
                     /locus_tag="ACHK49_004499"
     CDS             complement(<1..934)
                     /locus_tag="ACHK49_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFO9265007.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFSYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYA"
     gene            1155..1583
                     /gene="uspG"
                     /locus_tag="ACHK49_004500"
     CDS             1155..1583
                     /gene="uspG"
                     /locus_tag="ACHK49_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO9265008.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(1704..3269)
                     /gene="ahpF"
                     /locus_tag="ACHK49_004501"
     CDS             complement(1704..3269)
                     /gene="ahpF"
                     /locus_tag="ACHK49_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO9265009.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3440..4003)
                     /gene="ahpC"
                     /locus_tag="ACHK49_004502"
     CDS             complement(3440..4003)
                     /gene="ahpC"
                     /locus_tag="ACHK49_004502"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO9265010.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            4375..5121
                     /gene="dsbG"
                     /locus_tag="ACHK49_004503"
     CDS             4375..5121
                     /gene="dsbG"
                     /locus_tag="ACHK49_004503"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO9265011.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         1333 a         1325 c         1269 g         1339 t
ORIGIN      
        1 cagcgtagac gcccggtacg ctaacaatgc cgccacgcct gaccgccgca atacactggc
       61 gcaacgcttt accgctgctg ccctccagtt tcaggttagt cagcaccgtt tccgtggtgc
      121 tgcctttcgc ttcaaaaccg acggcgtcta ttactgcatc cacgccccgg tgacctgccg
      181 tttgttcaat aattgactgt gccggatcgc tatcttcatc gaaattaatc gggatcgcgc
      241 cgtagcggtc agcggcgaaa cgcaagcggt agggatgatg atcaacaaca aaaatctgtt
      301 ccgcaccgag caaccgtgca caggcgattg tcaacaatcc cactggaccg gcaccataga
      361 ctgcaacgct tgaaccttgt tggatctgcg cattttttgc tgcctgccat gccgttggca
      421 gaatatcaga aaggaaaagg gctttatcat ctgaaagcaa aggcggtact ttaaacggcc
      481 ccacattccc tttagggacg cggacatatt ccgcctgtcc accaggaacg ccaccataca
      541 ggtgactata actaaacaat gccgcgggag ctggtatctg ttttttattg agcgcagcgc
      601 ctttacccgc attggtattt tcgcaggcgg catattgctg cattcgacag aaaaaacagt
      661 cgccacaagc aatgacaaac ggaattacca cccggtcgcc tttttgcaaa ttttttacgt
      721 cctttccggt ttcaacgact tcccccataa attcatgacc aaaaatatcg ccatgtttaa
      781 cctggggtat tttgcctcga taaagatgga ggtcagagcc acagatcgcc gttgccgtaa
      841 tacgcagaat aatatcatct gcctgttcaa tgcccggatc gggaacattt tctacctgaa
      901 cgtgatgtgg gccgtgataa gtcaatgctt tcatgcaacc tccagagaca gttcaaaaaa
      961 gagtcagtct ttaagggtag ctatcacagc gggaaaacgg gcggtaccgc aggttttggg
     1021 agaattcctg gtgttcagtg agaatatatt aatcacagaa accgttcgta aggaaaaaga
     1081 accagacaaa tatcagggat aaatattata atcatggcga atgatttgtt catgattaac
     1141 agggagaaag ggttatgtat aagacaatca ttatgccagt tgatgtattt gaaatggaat
     1201 tgagcgacaa agctgttcgc cacgctgaat tcctcgccca ggatgacgga gttattcatc
     1261 tacttcacgt actacccggg tcagccagcc tgagcctgca ccgttttgcc gctgatgtgc
     1321 gtcgttttga agagcatctg caacatgaag cagaagaacg tctgcaaacg atggtcagcc
     1381 acttcaccat cgatccttcc cgcattaaac aacatgtccg ttttggtagc gtgcgggatg
     1441 aagtcaatga gttggcagaa gaactggggg ctgatgttgt agttattggt tctcgcaacc
     1501 catcgatttc gacccatctg ttaggttcta acgcctcgag cgtgatccgc catgccaatc
     1561 tgccggtgct ggttgtgcgt taattgccag aataaatatc ccgccctgcc cggagccatc
     1621 ccggcagggc tttttaatgc cataaaaaag ccgccaggtt tgaccccggc ggctaagcaa
     1681 ttgcaggtga atcttacttc ttcttatgca gttttggtgc gaatcaggta gtcaaaagca
     1741 ctcagagagg ctttggcacc ttcgcctgtg gcgatgataa tctgcttgta cggaaccgtc
     1801 gtacagtcac ccgctgcgaa cacgcctttg acgttggttt cgcatttcgc atcaatgata
     1861 atctcaccca tgcggttacg ttcgactgcg ccttccagcc agttggtgtt cggcagcaga
     1921 ccaatctgga cgaaaatacc ggccagttcg atgttgtgaa tatcgccgct gacacgatct
     1981 cgatattcca gaccaacgac tttgctgccg tcgcctttca cttccgtggt ttgcgcattc
     2041 agaataatgt cgacgttttt caggctgcgc agtttgtcct gcagaacctg gtcggctttc
     2101 atttctggcg caaattcgag cagcgttacg tgctcaacga tacccgccag gtcaattgcc
     2161 gcttccacgc cggagttacc gccgccgata accgctacgc gtttgccttt aaacagcggg
     2221 ccgtcgcagt gcgggcagta ggtcacgcct ttggtgcgat actgatcttc gccaggaacg
     2281 ttcatgttgc gccattttgc accggtcgcc acgataatgc tgcgtgcttt cagtaccgcg
     2341 ccagaagctg tttcaatctg atgcagacca ccttcaactg ctgctgggat cagtttgctg
     2401 gcgctctggc tgtcgatcac atcaacgtcg tattcatcaa cgtgaacttt cagtgcgcct
     2461 gccagcttct gcccttcagt cttcggtaca gaaatgtagt tttcgatatc aacggtatcg
     2521 aggatctgac caccaaaacg ttcgcccatc agaccggtac ggatgccttt acgtgcggag
     2581 taaattgctg cagctgcacc cgccgggccg gaaccaacga ttaatacgtc ataagcatca
     2641 cgcttgttca gctcttctgc cgcacgtttt tccgcgcccg tatcaatttt ggcaacgatt
     2701 tcagtcaacg tcatgcggcc ctgaccaaac tctttcccgt ttacgaacac tgccggaacg
     2761 cccatcacgt tgcgatcggt gatttcgttc tggaaggtgc cgccgtcaat tgcagtgtgc
     2821 ttgatgcgcg ggttcagtac gctcatcagg ttcagcgcct gcaccacgtc cgggcagtta
     2881 tggcaagaga gcgagtaata ggtttcgaat tcaaaatcac cgtcaatatg gcgaatctgc
     2941 tccagcagag actgcgcttc tttcgacgga tgaccaccgg tccatagcaa cgccagtacc
     3001 agcgaggtga actcgtggcc cagcggggag cctgcaaaac gtggcccctg gttggaacct
     3061 gggttggtga tcaggaaaga cggcttacgc accggcaagc tgttatcttc tttaaaggtg
     3121 actttgtctg acagttctgc gatttcagcc aacagttcct tgatttctgc cgatttagcg
     3181 ctgtcatcca gcgtggcaat taactcaaca ggcttggtca atttctcaag gtaagccttg
     3241 agttgagttt tcatatttgt gtcgagcatg tttatctcct gggctttaaa catcatcatg
     3301 caagcggcct ctgcggctac ctggatgcag cttgcatcat ggtgcgggga agaaaatcgg
     3361 gtgccgcagc acccgaagaa ttaggtgaat ttccgaagta tttcgcgccg gagttggtag
     3421 cgcgaaagac gaaggaaatt tagattttac caaccaggtc cagagacgga gccagagttg
     3481 cttcaccttc tttccattta gccgggcaaa cttcacctgg gtgagaagct acgtactgtg
     3541 ctgctttgat tttacgcagc aggtcagacg cgtcacggcc aatgccttca gcggtaactt
     3601 cgattgcctg gatgataccc tgcgggtcaa caacgaaggt cgcacggtca gccagacctt
     3661 catcttcacg catgttgtcg aagttacggg tcagggcgcc agtcgggtcg ccgatcatcg
     3721 catatttgat tttagcgatg gtttcagagc tgctgtgcca tgctttgtgg gtgaagtgag
     3781 tatcggtaga tactgcgtat acgtctacgc ccagtttctg cagttcttcg tagtggtcag
     3841 caacgtcacc cagttcggtc gggcatacga aagtaaagtc agccgggtag aagaagaaga
     3901 cgctccagcg gccttcggta tctttttcgg tgatttcgat gaattcgccg tttttgaatg
     3961 cctggttttt aaaaggttta attttggtgt taatcaagga catctatact tcctccgtgt
     4021 tttcgatgag atgtaaggta accaattttt gccgattcgg ctaatgcgtt tccattatca
     4081 aatcgataag ttttacctta caaccttcgt aagacaactt tgtgaacttt gcaccgccag
     4141 cggcgataaa aagtaaaatg gccccctttc caggtgaccg tgtaatctga actacccttt
     4201 ggtatcttca gtgcctgcct gcaggctgag gtgttgccct tacaaatgca acaacgacat
     4261 ggattacaac accctcataa acaaagggca atcacctgat ctaagctctt acctatgaca
     4321 gtgataggtt atgcctttta ctcgactttt gcactgactg aaaaggacaa attaatgtta
     4381 aaaaagatac ttttactggc tctgcttcct gcaatcgcct tcgcagagga acttcctgct
     4441 ccagtaaaag cgattgaaaa acagggcatt acaatcatca aaacattcga tgcccccgga
     4501 ggaatgaaag gttatctcgg aaagtatcag gatatgggcg tcaccatcta cctgactcca
     4561 gatggtaagc acgctatctc tggttacatg tacaacgaga aaggtgaaaa cctgagtaac
     4621 acacttatcg aaaaagaaat ttacgcacca gccggacgcg aaatgtggca acggatggaa
     4681 caatcccact ggctcctcga cggtaaaaaa gatgcgccgg tcattgtcta cgtgttcgcc
     4741 gatccgttct gcccatattg taaacagttc tggcagcagg cgcgcccgtg ggtagattct
     4801 ggcaaagtgc aattaagaac attgttggtt ggggttatca agccagaaag cccggcgaca
     4861 gcagcggcaa ttcttgcctc caaagatccc gcaaaaacct ggcaagaata tgaatcctct
     4921 ggtggcaagc ttaagctaaa cgtgcctgca aacgtaagta cagaacaaat gaaagtgtta
     4981 agcgccaacg agaaactcat ggacgatctg ggggcaaatg tcacaccagc gatctattac
     5041 atgagtaagg aaaatacgct gcaacaggcc gtggggttgc ccgatcagaa aacgcttaat
     5101 atcattatgg ggaataaata aaagataatt catggatggt tgaaatacat ccctgctttt
     5161 caaccatcct aaaaaagcat gaattgctcg ctctgcccta agtggctatg ctattttaga
     5221 aaaacttaat cctgagttaa gcaaaaaaat ataatcaata aaaata
//
LOCUS       DAZGLJ010000279         5230 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000279 DAZGLJ010000000
VERSION     DAZGLJ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.279"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..177
                     /locus_tag="ACHK49_004504"
     CDS             <1..177
                     /locus_tag="ACHK49_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO9265012.1"
                     /translation="RLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGR
                     SLKQEPTEMIQATA"
     gene            221..328
                     /locus_tag="ACHK49_004505"
                     /pseudo
     CDS             221..328
                     /locus_tag="ACHK49_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(312..950)
                     /gene="ytfB"
                     /locus_tag="ACHK49_004506"
     CDS             complement(312..950)
                     /gene="ytfB"
                     /locus_tag="ACHK49_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO9265013.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1169..1789
                     /gene="fklB"
                     /locus_tag="ACHK49_004507"
     CDS             1169..1789
                     /gene="fklB"
                     /locus_tag="ACHK49_004507"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO9265014.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2098..3501
                     /gene="cycA"
                     /locus_tag="ACHK49_004508"
                     /gene_synonym="dagA"
     CDS             2098..3501
                     /gene="cycA"
                     /locus_tag="ACHK49_004508"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO9265015.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRMAK"
     gene            complement(3543..3641)
                     /locus_tag="ACHK49_004509"
                     /pseudo
     CDS             complement(3543..3641)
                     /locus_tag="ACHK49_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3768..4196
                     /locus_tag="ACHK49_004510"
                     /pseudo
     CDS             3768..4196
                     /locus_tag="ACHK49_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246814.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4301..>5230
                     /locus_tag="ACHK49_004511"
     CDS             4301..>5230
                     /locus_tag="ACHK49_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265016.1"
                     /translation="MAILHPQECWLLERIMSPEYYRRRFEGWQEFVELCERQVAEWSK
                     TMPLDVRRRPLCEQIDVVWGGRVLPNIRSTLKSVQYDFIQLQQGDLRVLQSGGNISSD
                     MKGLIDYPSDWMSLAAQKQYDRLKWRGAHYNNLIRRTSGGYWYDGELTYYYEESLHGP
                     LALPMQLPLYELDSSVYLREDDSVTVAGLYLPDIPDASAQLLYRSEHIPEAWQGRVRT
                     KYVNEAGIQEYYWESGAWAKCNWKRIRRVANRFINVPPEGFFPQGMPEELYNWPQREA
                     QYVTDRQRIAAFSGEACPHSGEWSIFVEGRQATV"
BASE COUNT         1171 a         1265 c         1440 g         1354 t
ORIGIN      
        1 cgcctgtcac aaagtaaatt cagatgccag gtatgtggat atacagcgaa cgccgatgta
       61 aacggcgctc gtaacatttt agcggcgggg cacgccgttc ttgcctgtgg agagatggtg
      121 cagtcaggcc gctcgttgaa gcaggaaccc accgaaatga ttcaggcgac agcctgaacg
      181 aagcagggat ccacgtcctt cagggcgtgg aggatgtcaa catatcagta tggtttcccc
      241 ccaggcactg gaagccagac gtaaggtcat caaagagaca atccttagct ggcttttggt
      301 tgacccttca tctaccgcgc acgaataaaa ctgccatccg gctggcgggt aaacaggacc
      361 tgttgattat tccccgtatc aatggttaag cccgtcacca cgccgctggc gttctggcgg
      421 attttcacca tttgcccgtt ttgcaaatta ctcagcggct tacccgcgcc ttctacctgc
      481 gccattgcat agacatcggt agcgggcagt ccgtggtcgc ggaacagctg cgccatcgtt
      541 ttaccgggtt cgacacggta agagcgccat tgattatcaa ttccgctatc tggctggaat
      601 ggttgcgttt gggtagtttg cggctgctct tctggctggc cttcctgtat aggttctgga
      661 gcgaccggag ccacctgatc aggatcattt tgtggggtga ctaattgcgc ccgcagctgt
      721 tcttctgtcg gcggttgtga ttgcgactga atatctaact gcgcttcacg cgtcaccacc
      781 ggcgcgttgg gcgtatcatc agatggaagc agaaatccga ccaccaatac aatggcggca
      841 ataatgatcc cccgacgatg catcggcggc agcgggtcca tgaagcgaaa attgtccggc
      901 gcgtgccaga ctttctccag ggttggtttt agttcaaagc gcccgggcat ggtttcctcc
      961 tgctccgcgt cttgttcctc aatcatagcc tatgaataag ctaacgctat gatgtccgtg
     1021 gtaaacccgc ctttattata ttcatacggg atattgctat tgtttctttt tccccgggat
     1081 ttgtcatcat tcctgcgaca aagttttacc caaagaagtg tggctgatat gctgcccgct
     1141 acttttatac cctaagaaag gaaatacgat gaccacccca acttttgaca ccatcgaagc
     1201 gcaagcaagc tacggcattg gtttgcaggt agggcaacaa ctgagtgaat ctggcctgga
     1261 agggctgctg ccagaagcac tggttgcagg tattgccgat gcgctggaag gcaaacatcc
     1321 ggctgttccg gttgatgtgg tgcatcgcgc gctgcgtgaa atccacgagc gcgccgatgc
     1381 cgttcgtcgt cagcgtttcc aggcgatggc tgctgaaggt gtgaaatacc tggaagaaaa
     1441 cgccaaaaaa gaaggtgtga atagcaccga atctggcctg caattccgcg tgatcaacca
     1501 gggtgaaggc gcaattccgg cacgtaccga ccgcgttcgt gttcattaca ccggtaaact
     1561 gatcgacggc accgtgtttg acagctccgt tgctcgtggt gaacccgctg aatttccggt
     1621 taatggcgtg atccctggct ggattgaagc actgactctg atgccggtag gttctaaatg
     1681 ggaactgact atcccgcagg aactggcata tggcgagcgc ggcgcaggcg catccatccc
     1741 tccgttcagc accctggtgt ttgaagtcga actgctggaa atcctctaag cagcgcattc
     1801 tgttcccctc gaaccagagg ggagcaggca ttcagcaata aacccttcag tttgccaaac
     1861 ggcgctattt tgtgttgcaa agaccccgta agcgtgtatt tttgtgagct gtttcgcgtt
     1921 atcaccgtga tatgacactc actttaaaca taaaattaac attagatcta aatcttagta
     1981 ttcatcccgc gtattgttac ctaatatcga tgagtcccga tacagattcg tcgtatcata
     2041 ggctgactaa aggccgtaga gcctgaacaa cacagacagg tacaggaaga aaaaaacatg
     2101 gtagatcagg taaaagtcgt tgccgatgat caggctccgg ctgaacagtc gctacggcgc
     2161 aatctcacaa accgacatat tcagcttatt gccattggcg gtgccattgg tacggggttg
     2221 tttatggggt ctggcaaaac gattagcctt gccgggccgt cgatcatttt cgtttatatg
     2281 atcatcggtt ttatgctctt tttcgtgatg cgggcaatgg gggaattgct gctttcgaat
     2341 ctggaataca aatcttttag tgacttcgct tccgatttac tcgggccgtg ggcaggatat
     2401 ttcaccggct ggacttactg gttctgctgg gttgtaaccg gtatggcaga cgtggtggcg
     2461 atcacggctt atgctcagtt ctggttcccc gatctctccg actgggtcgc ctcgctggcg
     2521 gtgatagtgc tgctgctgac gctcaatctc gccaccgtga aaatgttcgg tgagatggag
     2581 ttctggtttg cgatgatcaa aatcgtcgcc attgtgtcgc tgattgtcgt cggcctggtt
     2641 atggtggcga tgcactttca gtcaccgact ggtgtggaag cgtcattcgc gcatttgtgg
     2701 aatgatggcg gctggttccc gaaaggttta agtggcttct ttgccggatt ccagatagcg
     2761 gttttcgctt tcgtagggat tgagctggta ggtacaaccg ctgcggaaac caaagatccg
     2821 gagaaatcac tgccgcgtgc aattaactct attccgatcc gtatcattat gttctacgtc
     2881 ttcgcgctga ttgtaattat gtccgtgacg ccgtggagtt cggtagtccc ggagaaaagc
     2941 ccgtttgttg aactgttcgt gttggtgggt ttgcctgcgg ctgccagcgt gatcaacttt
     3001 gtggtgctga cctctgcggc gtcttccgct aacagcggcg tcttctctac cagccgtatg
     3061 ctgtttggcc tggcccagga aggtgttgca ccgaaagcgt tcgctaaact ctctaagcgc
     3121 gcagtacccg cgaaagggct gaccttctcc tgcatctgtc tgctcggcgg cgtggtgatg
     3181 ctgtatgtga atcccagcgt gattggcgcg ttcacgatga tcacgacggt ttccgcgatt
     3241 ctgtttatgt tcgtctggac gattatcctt tgctcgtacc tggtgtatcg caaacagcgt
     3301 cctcatctgc atgagaagtc gatctacaag atgccgctcg gcaagctgat gtgctgggta
     3361 tgtatggcgt tctttgtgtt cgtgatcgtg ctgctgacac tggaagatga cactcgccag
     3421 gcgctgctgg ttaccccgct gtggtttatc gcgctggggt tgggctggct gtttattggt
     3481 aaaaaacgca tggcgaagta atgccgctaa ggcacctgcg ggtgtctttc aggctgcaaa
     3541 attgcctgat gcgctacgct tatcaggcct acagaacctc tgcaatatat tgagtttgcg
     3601 tgcttttgta ggccgggtaa ggcgtttacg ccgcatccgg catgaacaaa gcacactttg
     3661 tcagcaatct cctttgtctt tatacattcc taaaaatact tcccgtcatc gacagctctc
     3721 aggcgcgggc ttaccgtgga tctttgccac tcattgcaga gttcactatg gctaagcaca
     3781 gttggttatt aatcgccgca ttacctctct ccatttcacc ttcctggggc gcagattttt
     3841 attaccgcca gcaggagaaa ggcacggttt atgttgccga acagaaggga gaaaaggatg
     3901 agatcctctc cgaactgccg ggcgttaatt tttcccgcct ctggcgtatt gccaatttag
     3961 ccaataacca agaaacccgc ttactgtccg attttaatcc cgataagttc gattgcgatg
     4021 atcggaataa ttgccagcat acctggctca ccgatggacg ctctgttctt tggtctggca
     4081 aagtcctgaa aaatccgccc ggtgaaccta atgtcgatgc cgccagtttt caggcattcg
     4141 gtgctttcgc tgccgataaa cgcagcgtct attttgacgg tcagcgtacc gatgatagat
     4201 gaagtggata tgtatatagc tgtgtctcag ttgcaaacaa atatttatgt accgaaataa
     4261 aagtatcaat agacaattaa ttaataaatc aggagataaa gtggctatat tacatccaca
     4321 ggagtgctgg ttgctggaaa gaataatgtc gccggaatat tatcggcgtc gttttgaggg
     4381 ctggcaggag tttgtcgagc tgtgcgagcg acaggtggca gagtggagca agaccatgcc
     4441 gctggatgtg cgccgtcgtc cgctgtgcga gcagattgac gtcgtctggg gcgggcgagt
     4501 gttgcccaat atccgcagta ctttaaagtc ggtacagtat gactttattc agctacagca
     4561 gggggacctg cgggtactgc aatccggggg caatattagc agtgatatga aaggactaat
     4621 tgattatccg tcggactgga tgtcactcgc cgcgcaaaaa cagtatgaca gactgaaatg
     4681 gcgaggtgca cattataata atttaatcag acgtacctcg ggcggatact ggtatgacgg
     4741 cgaactgact tattattatg aggaaagtct gcacggcccg ctggccctgc cgatgcaatt
     4801 accgttgtat gagctggata gtagcgtata cctgcgtgag gatgactcgg taacggtggc
     4861 tggcctgtat ctgccggata tcccagatgc cagcgcccaa ctgttgtacc ggagtgaaca
     4921 tattcccgag gcttggcagg ggcgggtacg aacgaagtat gtcaacgagg ctgggataca
     4981 agaatattac tgggaaagcg gggcgtgggc aaaatgcaat tggaaacgta tacggcgggt
     5041 ggcaaaccga tttatcaatg tgccgccgga gggatttttc ccacagggca tgccggagga
     5101 gttgtacaac tggccacagc gcgaagcgca gtatgttaca gaccggcagc ggatagcggc
     5161 ctttagcggt gaagcttgtc ctcatagcgg ggagtggtcg atttttgtag aaggtagaca
     5221 agcgacggtg
//
LOCUS       DAZGLJ010000280         5214 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000280 DAZGLJ010000000
VERSION     DAZGLJ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.280"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            49..288
                     /locus_tag="ACHK49_004512"
                     /pseudo
     CDS             49..288
                     /locus_tag="ACHK49_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(336..953)
                     /gene="ureG"
                     /locus_tag="ACHK49_004513"
     CDS             complement(336..953)
                     /gene="ureG"
                     /locus_tag="ACHK49_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFO9265017.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLDLIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(965..1639)
                     /locus_tag="ACHK49_004514"
     CDS             complement(965..1639)
                     /locus_tag="ACHK49_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFO9265018.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(1640..2104)
                     /gene="ureE"
                     /locus_tag="ACHK49_004515"
     CDS             complement(1640..2104)
                     /gene="ureE"
                     /locus_tag="ACHK49_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFO9265019.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(2114..3784)
                     /gene="ureC"
                     /locus_tag="ACHK49_004516"
     CDS             complement(2114..3784)
                     /gene="ureC"
                     /locus_tag="ACHK49_004516"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFO9265020.1"
                     /translation="MFGPTTGDKIRLADTELWIEVEDDLTTYGEEVKFGGGKVIRDGM
                     GQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNPDIQPNVTIPIG
                     VSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTGPTAGSNATTCT
                     PGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKIHEDWGATPAVI
                     NCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGAGGGHAPDIITA
                     CAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAESRIRQETIAAE
                     DVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEESGDNDNVRVKRY
                     IAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKGGMIAMAPMGDI
                     NGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLHSTTAVVKGCRT
                     VQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFLF"
     gene            complement(3810..4130)
                     /locus_tag="ACHK49_004517"
     CDS             complement(3810..4130)
                     /locus_tag="ACHK49_004517"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFO9265021.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVDLVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(4139..4441)
                     /locus_tag="ACHK49_004518"
     CDS             complement(4139..4441)
                     /locus_tag="ACHK49_004518"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFO9265022.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(4451..>5214)
                     /locus_tag="ACHK49_004519"
     CDS             complement(4451..>5214)
                     /locus_tag="ACHK49_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFO9265023.1"
                     /translation="QRLHGKTTLTTRHHVGPLTVQRPFYPEEETCHLCLLHPPGGIVG
                     GDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLPQDSIFF
                     PGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGSPLLIER
                     LHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGLLTVRFL
                     SDDNLRCQQVMRDIWQFMRPQLTDKSPVLPRIWLT"
BASE COUNT         1142 a         1405 c         1423 g         1244 t
ORIGIN      
        1 ctgtagattc aatctgtcaa tgcaacaccc ctttcaatta tctctttcac tgcccgcgac
       61 ggtatcggtt atcagtttga cctgctggct aacggtgaaa ccgtactgcg tgatgcatcc
      121 ggcgaaaaac gcatcaaggg tgaaaaagac ggtcgtatgc tcatgaatgt tggcctgaat
      181 gcggaaattc gcgacaacat ccgcttcggt ctggagtttg aaaaatcggc atctggtaag
      241 tacaacgtgg ataacgcggt gaacgccaac ttccgttatt ccttctgata aaaacaaggg
      301 gccgcctgcc ggccctgata cagaggatgt tgtgattact tacccagcat gccctgctct
      361 tcgataaagg cgatgatgtt ttgcagacct tcaccacttt tcaggttagt gaacccccat
      421 gggcgttctc cgcgcatacg taaagtatca cgctccatta cctcgaggga ggcgcccacg
      481 tatggtgcaa ggtcagtttt attgatcacg aggaagtcgg atttggtgat ccccggaccg
      541 cctttacgcg ggatcttttc tccttctgcc acatctatga catatatggt aaggtccgcc
      601 agttcggggc tgaaggtcgc actcaggttg tcgccgccgc tttccacaaa gatcagatcg
      661 agattaccaa atttctcact cagtgcttcc acggctgcca gattcattga tgcgtcttca
      721 cgaatggctg tatgagggca gccccccgtt tcgacaccga caatgcgttc cgcttccagt
      781 gctccggcct cagtgagaat gcgttgatct tctctggtgt agatgtcgtt ggtgactacg
      841 gctaattgcc aggtgtcacg catggatttg cagagggctt ccagaagcgc ggttttacct
      901 gagccgaccg gaccgccgac gccgacacgc agcggttgct tgattatgtt cattttattt
      961 tctcctaaga acggaataat cgggaatact gggtttcatg tcgcgcggaa gcgatagatg
     1021 caagtggcgt tgctgaacca attgcgtgat ccggggttgc aagggcactg tccatgttcg
     1081 ccgcatagcg ggcacacagg cgcataatta actgctgggc agccagctgg ccgtagggta
     1141 ccagcctgac gcccgccatc actgcgcttt caatccagct gtagccaagg ctcagggcca
     1201 ggtctgaaac ggatatttgc cagcgtacgc cgagccatgc cataccggtt aactggcttt
     1261 gctggcatag tttgcgccat tcggccgggc agtcgggctg ccagtcgacc agaagtctgg
     1321 tgaaggctgc accgcggtta cgttcttcat cgcgtagctc acgggtttcc ctgcaggcca
     1381 gcagataagc ggtccagcgc ccggcactgt caggatcgcc cgcttcacag gcccggtata
     1441 gccgggcaag cagtggcagg tcgacggcaa aaaaactctg ttccatctgc aactgctgcc
     1501 agtgttcaaa ggcggcgcta tcttctaccc agcctgcttc cactgcccac tccagtccct
     1561 gggaccagct gtagcctcca acagggaggc tgttactggc aagctgcatc agccgcagac
     1621 gtttttccgg agtcggcatt cagtggctgt gctcctgatc gtgattatgg gcgtgcgact
     1681 tgctggcgta agcgccagct tccggctcaa acggaagatg tgcaaaatca acatccagcc
     1741 caaactggcg cagcatatca tcgaggacgt gatcgtgatg gtagcgcagc tctccgggca
     1801 ttatttgcag cggaacgtgg cgattgccga gatgccagca ggctttcgcg agcataaaag
     1861 gatctgcgca gcgcactaca gaaacggctt catcagcggc gatcacctta atgaattcat
     1921 caccgttttc gttactgaga atatcaccat cgcgcagcag cagaccgcgt ggcagcaaca
     1981 gtccagcctg acgtccatcg ttaagggtga ctttaatacg gctttttacc cgcatgtcaa
     2041 cagggagggt aacgctggca gttgtctgag ccggggtctc cacccgccgg gtcaaataaa
     2101 gcatttgtgc tccttaaaac aggaaataac gttgcgccat tggcagaatg tccgccggtt
     2161 cactggttat cagttcgccg ttgatacgca cttcgtaggt ttgtgaatcc acggttatat
     2221 caggcaacag gctgttgtgg cgcatatcgg ctttttgtac tgtgcggcag ccttttacca
     2281 cagcagttgt gctgtgcagg ttaagctgtt cagcgacgcc atttgctgct gctgcctgcg
     2341 acaggaaagt cacacgacag cggtgacggg cactgcccaa tgcagcgaac attgggcgat
     2401 agtgcaccgg ctgcggtgtg gggatagagc cgttgatatc acccatcggc gccatggcta
     2461 tcattccgcc tttgacgata gtcgccggtt ttacgccaaa gaacgccggg gaccagagca
     2521 ccagatccgc cagttttccc acttcaatcg agccgacttc atgagcaata ccgtgggtta
     2581 atgccggatt aatggtgtat ttagcgatat agcgcttcac gcggacgttg tcgttatcac
     2641 cactttcttc cggtaacggg ccgcgctgaa ctttcatccg gtgtgccacc tgccaggttc
     2701 gtaacactac ttctccgacg cgtcccatgg cctgcgaatc ggacgaggtg agggagaacg
     2761 cgccaaggtc gtgcaggacg tcttccgcgg caatggtttc ctggcgaatg cgcgattcgg
     2821 caaaggctac gtcctcggcg atatccgggt caagatgatg gcaaaccatc agcatgtcca
     2881 gatgctcatc aatagtgttg acggtatagg gtagcgtcgg attggttgag gaaggcagaa
     2941 tattggggtg cgcgcaggcg gtgataatat ccggagcatg accaccacct gcaccttctg
     3001 tatggaaggt atggatagtg cgcccgccga tggcagtcag agtatcctca acgaatcctg
     3061 attcgttaag cgtatcactg tgtagcgcaa cctgaacgtc catttcgtcg gctacagtca
     3121 gtgcgcagtt gattaccgca ggtgtagctc cccagtcttc gtgaattttg aggccgataa
     3181 ccccggccgc gacctgctca cgaagcgcat ccggattgga gcaattgcct ttacccagca
     3241 acccgatatt gaccggcagg ctgtctgcag cctgcagcat ttgataaatg taccatggtc
     3301 ctggggtaca ggttgtggcg ttagaacccg ctgtcgggcc agtaccgcca ccgatcatgg
     3361 tggtaatgcc ggatgtcagc gcttcttcag cctgctgtgg gcagatccag tgaatatgcg
     3421 tatcgacgcc acctgcggta acgatcctgc cttctgcggc aataatttcc gtggatacgc
     3481 cgattgggat agtgacgttg ggttgtatat caggattacc ggctttgccg atagcaaata
     3541 tccttccatc tttgacgccg atatcggctt taacgatccc ccagtaatcg atgatcaggg
     3601 cattggtcag caccagatca gcgcagccgg cggagagcat ttgcccctgt cccataccgt
     3661 cgcggattac tttaccgccg ccgaatttga cctcttcgcc gtaggtagtt aaatcatctt
     3721 cgacctcgat ccacagctca gtgtctgcca gacgaatttt atcaccggtg gtagggccga
     3781 acatgtcagc ataggcctag cgtgaaatat tactcatcat ttgcctccag ctcacccatt
     3841 atatctccgc ggaaaccgtg cacgatacgg gcaccgctca gcgccaccag atcaacctca
     3901 cgcttttggc ctggttcgaa gcgcactgca gttccggcgg cgatattgag acgatagcct
     3961 cttgccttct ggcgatcgaa cttcagtgcc gggttaacct ccgcgaagtg ataatgggaa
     4021 ccgacctgaa ttggtcggtc tccgtgattt tcaacgataa tactgcaggt agcccggccg
     4081 atgttaagct ctatatagcc aggtttaacc ttatattctc cagggatcat ctgttacctc
     4141 agattatcgg attatggacg gtaaccagct tggagccgtc cggaaaggtg gcttcgacct
     4201 ggatatcggg gatcatttct ggtatgcctt ccatgacctg ctcgcgactc aggacatgcc
     4261 gtccttcttc catcagcgca gcgacgcttt tgccgtcgcg agcgccctcc attataaaag
     4321 cgctaatcag ggccacggat tcgggatagt taagtttcag gccgcgggcc agacgacgct
     4381 ctgccagcag tgcagcggta aacagtaata gtttgtcttt ttctcttgga gtcagttcca
     4441 tagcgttctc ttaagtcagc cagatccggg gaagtaccgg tgatttgtca gtcagttgcg
     4501 gccgcataaa ttgccagata tcacgcatta cctgctggca acgcagatta tcatcactta
     4561 aaaagcgaac tgtcagtaga ccatcggtga gcgtggctcc ggcgtagttt tccagtggtg
     4621 tgagtttttc gcgtatgtct tccagttgcg tttcattccc caggtagaac agcatcgttc
     4681 ccacccatgg tttccgggca acgcaggtaa gttctccatt ggccacgtgc agacgctcaa
     4741 tcaacagcgg agaaccgtcg acccatatct ccagacggtt agcgagtgtt ccgtggctga
     4801 aggtttctcc gatgaccgga cgtccaaggc acagaagatc ccaggccagc agtgtactgg
     4861 aggacgacag atgaaacacg gtatgcaacg tggcgtgagc gccgggaaaa aaaatcgaat
     4921 cctgtggcaa ccactccagc gttgcgttat tgcacagcgt caggttttgc tgaaggtgcg
     4981 cctgcgcgcc gctgctgcgg taaaatttac tggcaccagg catggtgatc agcgtatggc
     5041 aatcagagtc aattattgcg ttgatgatga gttcgtcacc gccgacgata ccaccgggtg
     5101 gatgaagcag acagaggtgg caggtttctt cttcgggata aaaaggacgc tgaacagtga
     5161 gcggaccgac atgatgacgg gtggtgagcg tggttttccc gtgcagacgc tgaa
//
LOCUS       DAZGLJ010000281         5211 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000281 DAZGLJ010000000
VERSION     DAZGLJ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.281"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1820
                     /locus_tag="ACHK49_004520"
     CDS             <1..1820
                     /locus_tag="ACHK49_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO9265024.1"
                     /translation="NYYLPKESAYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARV
                     GYSKMLLGVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGK
                     KHRDNTLTMKRFSNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFL
                     GDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDK
                     ETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSS
                     GEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIG
                     ERPDAEVMAERKEYYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQII
                     MGRHDDEQTLLRVDEAINKTYTRRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRV
                     IPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVH
                     FPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRISI
                     SRWQLDLSALLASLQEEDGAATNKKTLAEYARALSGEDE"
     gene            1817..2023
                     /locus_tag="ACHK49_004521"
     CDS             1817..2023
                     /locus_tag="ACHK49_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO9265025.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2020..3621
                     /locus_tag="ACHK49_004522"
     CDS             2020..3621
                     /locus_tag="ACHK49_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9265026.1"
                     /translation="MKMSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTR
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYSAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            3602..4921
                     /locus_tag="ACHK49_004523"
     CDS             3602..4921
                     /locus_tag="ACHK49_004523"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO9265027.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAM
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            4931..>5211
                     /locus_tag="ACHK49_004524"
     CDS             4931..>5211
                     /locus_tag="ACHK49_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO9265028.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKASAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAAS"
BASE COUNT         1178 a         1388 c         1611 g         1034 t
ORIGIN      
        1 ccaattacta tctcccgaaa gaatccgcat accaggaagg gcgctgggaa acactgccct
       61 ttcagcgggc catcatgaat gcgatgggca gcgactacat ccgcgaggtg aatgtggtga
      121 agtctgcccg tgttggttat tccaaaatgc tgctgggtgt ttatgcctac ttcatagagc
      181 ataagcagcg caacacactt atctggttgc cgacggatgg tgatgccgag aactttatga
      241 aaacccacgt tgagccgacc atccgcgata ttccgtcgct gctggcgctg gctccgtggt
      301 atggcaaaaa gcaccgggat aacacgctca ctatgaagcg tttttccaat ggtcgtggct
      361 tctggtgcct gggcggtaaa gcggcaaaaa actaccgtga aaagtcggtg gatgtggcgg
      421 gttatgatga actggctgcc tttgatgagg atattgaaca ggaaggctct ccgacgttcc
      481 tgggcgataa gcgtattgaa ggctcggtct ggccaaagtc catccgtggc tccacgccca
      541 aagtgagagg cacctgccag attgagcgtg cagccagtga atccccgcat tttatgcgtt
      601 ttcatgttgc ctgcccgcac tgcggggagg agcagtatct taaatttggc gacaaagaga
      661 cgccgtttgg cctcaaatgg acgccggatg acccctccag cgtgttttat ctctgcgagc
      721 ataatgcctg cgtcatccgc cagcaggagc tggactttac tgatgcccgt tatatctgcg
      781 aaaagaccgg gatctggacc cgtgatggca ttctctggtt ttcgtcatcc ggtgaagaga
      841 ttgaaccgcc tgacagtgtg acctttcaca tctggacggc gtacagcccg ttcaccacct
      901 gggtgcagat tgtcaaagac tggatgaaga cgaaagggga tacgggaaaa cgtaaaacct
      961 tcgtgaacac cacgctcggt gagacgtggg aagcgaaaat cggcgaacgt ccggatgctg
     1021 aagtgatggc agagcggaaa gagtattatt cagcgcccgt tcctgatcgt gtggcttacc
     1081 tgaccgccgg tatcgactcc cagctggacc gctacgaaat gcgcgtatgg ggatgggggc
     1141 cgggtgagga aagctggctg attgaccggc agattattat gggccgccac gacgatgaac
     1201 agacgctgct gcgtgtggat gaggccatca ataaaaccta tacccgccgg aatggtgcag
     1261 aaatgtcggt atcccgtatc tgctgggata ctggcgggat tgacccgacc attgtgtatg
     1321 aacgctcgaa aaaacatggg ctgttccggg tgatccccat taaaggggca tccgtctacg
     1381 gaaagccggt ggccagcatg ccacgtaagc gaaacaaaaa cggggtttac cttaccgaaa
     1441 tcggtacgga taccgcgaaa gagcagattt ataaccgctt cacactgacg ccggaagggg
     1501 atgaaccgct tcccggtgcc gttcacttcc cgaataaccc ggatattttt gatcttaccg
     1561 aagcgcagca actgactgct gaagagcagg tcgaaaaatg ggtggatggc aggaaaaaaa
     1621 tactgtggga cagcaaaaag cgacgcaatg aggcgctcga ctgcttcgtt tatgcgctgg
     1681 cggcgctgcg catcagtatt tcccgctggc agctggatct cagtgcactg ctggcgagcc
     1741 tgcaggaaga ggatggtgca gcaaccaaca agaaaacact ggcagaatac gcccgtgcct
     1801 tatccggaga ggatgaatga cgcgacagga agaacttgcc gctgcccgtg cggcactgca
     1861 tgacctgatg acaggaaaac gggtggcaac ggtacagaaa gacggacggc gagtggagtt
     1921 tacgaccact tccgtgtctg acctgaaaaa atacattgct gagctggaag tgcagaccgg
     1981 catgacacag cgacgcaggg gaccagcagg attttatgta tgaaaatgtc caccattccc
     2041 acccttctgg ggccggacgg catgacatcg ctgcgtgaat atgccggtta tcacggcggt
     2101 ggcagcggat ttggtgggca gttgcgggcg tggaacccac cgggtgaaag tgtggatgca
     2161 gccctgctgc ccaactttac ccgtggcaat gcccgcgcag acgatctggt acgcaataac
     2221 ggctatgccg ccaacgccat ccagttgcat caggatcata tcgtcgggtc ttttttccga
     2281 ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt
     2341 tcccgcgagg ttgaagcggc atggaaagag tttgccgaag atgactgttg ctgcattgac
     2401 gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt
     2461 aacggtgaac tgttcgttca ggccacctgg gatacccgtc cctcgcgact gttccggaca
     2521 cagttccgga tggtcagccc gaagcgcatc agcaacccga acaataccgg cgacagccgg
     2581 aactgccgtg ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc
     2641 gaggacgggt atcctggctg gatgccgcag aaatggacat ggataccccg cgagttaccc
     2701 ggcggtcgtg cttcgttcat tcacgtcttt gaacccgtgg aggacgggca gacccgcggt
     2761 gcaaatgtgt tttacagcgt gatggagcag atgaagatgc tcgacacgct gcagaacacg
     2821 cagctgcaga gcgccattgt gaaggcgatg tatgccgcca ccattgagag tgagctggat
     2881 acgcagtcag cgatggattt tattctgggc gcgaacagtc aggagcagcg ggaaaggctg
     2941 accggctgga ttggtgaaat tgccgcgtat tactccgcag caccggtccg tctgggaggc
     3001 gcaaaagtgc cgcacctgat gccgggggac tcactgaacc tgcagacggc tcaggacacg
     3061 gataacggct actccgtgtt tgagcagtca ctgctgcggt atatcgctgc cgggctgggt
     3121 gtctcgtatg agcagctttc ccggaattac gcccagatga gctactccac ggcacgggcc
     3181 agtgcgaacg agtcgtgggc gtactttatg gggcggcgaa aattcgtcgc atcccgtcag
     3241 gcgagccaga tgtttctgtg ctggctggaa gaggccatcg ttcgccgcgt ggtgacgtta
     3301 ccttcaaaag cgcgcttcag ctttcaggaa gcccgcagtg cctgggggaa ctgcgactgg
     3361 ataggctccg gtcgtatggc catcgatggt ctgaaagaag ttcaggaagc ggtgatgctg
     3421 atagaagccg gactgagcac ctacgagaaa gagtgcgcga aacgcggtga cgactatcag
     3481 gaaatttttg cccagcaggt ccgtgaaacg atggagcgcc gcgcagccgg tcttaaaccg
     3541 cccgcctggg cggctgcggc atttgaatcc gggctgcgac aatcaacaga ggaggagaag
     3601 agtgacagca gagctgcgta atctcccgca tattgccagc atggctttta atgagccgct
     3661 gatgcttgaa cccgcctatg cgcgggtttt cttttgtgcg cttgcaggcc agcttgggat
     3721 cagccgcctg acggatgcag tatccggcga cagcctgact gccggagagg cacccgcggc
     3781 gctggcgtta tccggtgatg atgacggacc acgacaggcc cggagttatc aggtcatgaa
     3841 cggcatcgcc gtgctgccgg tgtccggtac gctggtcagc cggacgcggg cgctgcagcc
     3901 gtattcgggg atgaccggtt acaacggcat tatcgcccgt ctgcaacagg ctgccagcga
     3961 cccgatggtg gacggcattc tgctcgatat ggacacaccg ggcggaatgg tggcgggagc
     4021 atttgactgt gctgacatca tcgcccgtgt gcgtgacata aaaccggtat gggcgctggc
     4081 caacgacatg aactgcagtg caggtcagct gcttgccagc gccgcctccc ggcgtctggt
     4141 cacgcagacc gcccggacag gctccatcgg cgtcatgatg gctcacagta attacggtgc
     4201 tgccctggag aaacagggcg tggaaatcac gctgatttac agcggcagcc ataaggtgga
     4261 tggcaacccc tacagccatc taccgggtga tgtccgggaa acactgcagt cccggatgga
     4321 tgcaacccgc cggatgtttg cgcagaaggt gtcggcatat accggcctgt ccgtgcaggc
     4381 tgtgctggat accgaggctg cagtgtacag cggtcaggag gccattgatg ccggactggc
     4441 tgatgaactt gtcaacagca ccgatgcgat caccgttatg cgtgatgcac tggatgcacg
     4501 taaatcccgt ctctcaggag ggcgaatgac caaagagact caatcaacaa ctgtttcagc
     4561 cactgcttcg caggctgacg ttactggcgt ggtgccagcg atggagggcg aaaacgccag
     4621 cgcggcgcag ccggacgtga acgcgcagat caccgctgcg gttgcggcag aaaacagccg
     4681 cattatgggg atcctcaact gtgaggaggc tcacggacgc gaagaacagg cccgcgtgct
     4741 ggcagaaacc cccggtatga ccgtggaaac ggcccgccgc attctggccg cagcaccaca
     4801 gagtgcacag gcgcgcagtg atactgcgct ggatcgtctg atgcaggggg caccggcacc
     4861 gctggctgca ggtaacccgg catctgatgc cgttaacgat ttgctgaaca caccagtgta
     4921 agggatgttt atgacgagca aagaaacctt tacccattac cagccgctgg gcaacagtga
     4981 cccggctcat accgcaaccg cgcccggcgg attgagtgcg aaagcgtctg caatgacccc
     5041 gctgatgctg gacaccgcca cccgtaagct ggttgcgtgg gatggcacca ccgacggtgc
     5101 tgccgttggc attcttgcgg ttgctgctga ccagaccagc accacgctga cgttctacaa
     5161 gtccggcacg ttccgttatg aggatgtgct ctggccggag gctgccagcg a
//
LOCUS       DAZGLJ010000282         5139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000282 DAZGLJ010000000
VERSION     DAZGLJ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.282"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..833)
                     /locus_tag="ACHK49_004525"
     CDS             complement(<1..833)
                     /locus_tag="ACHK49_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9265029.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRNQTSESLGQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWATINNVADALPETVLRRSLGLRAEKIRSVYRESDIVPGEQTATSMLKQR
                     TKNIALLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPRREEMPVYTRWVKTQKCM
                     T"
     gene            complement(835..1107)
                     /locus_tag="ACHK49_004526"
     CDS             complement(835..1107)
                     /locus_tag="ACHK49_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265030.1"
                     /translation="MVHKTKFHNSDTDLAIRHGRRSHAFKTDWYQHPPCTEEQAEWLI
                     HNYRRRGYEIKKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            1054..1233
                     /locus_tag="ACHK49_004527"
     CDS             1054..1233
                     /locus_tag="ACHK49_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265031.1"
                     /translation="MPDGKVGIRIVKFGFVHHLFSLLAQQVSGVQADVRIVNQNARKK
                     QNPPKRVKCGCVEDA"
     gene            complement(1275..1430)
                     /gene="hokD"
                     /locus_tag="ACHK49_004528"
     CDS             complement(1275..1430)
                     /gene="hokD"
                     /locus_tag="ACHK49_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO9265032.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1988..3085
                     /locus_tag="ACHK49_004529"
     CDS             1988..3085
                     /locus_tag="ACHK49_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HFO9265033.1"
                     /translation="MTISYIPILKAKRSELSALSQLSIEKKSKILPLLEIEPVPIDPD
                     SGIALKSYNETLIEFGKKVSKSCSDMQGVYIDGLLIEEHFISPEDHYPIINAVNQVRD
                     MEIRVIPVSSPTRPSNYKRAIDELMQNEICLRLTTLDLVNPQLITHYINHLGIPLSNI
                     DIIIDLRDELTEDKINSGELYTLAMGLINNLAHLNEYRKVILSGGSFPTDLSDISVGL
                     YSQPRIEWILWQSLMNRKELARNVIYSDYGVQHPDFNRLSTRFPSVSASVRYSGDNDF
                     WVFRGRVANRFGYEQYGKHSEDILAHREYSGPTFCAGDRDIEYYANEYQAYKANPSGN
                     YKFGSPEVWRRIGQNHHITKVVEQLATLYGL"
     gene            complement(3045..3623)
                     /locus_tag="ACHK49_004530"
     CDS             complement(3045..3623)
                     /locus_tag="ACHK49_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HFO9265034.1"
                     /translation="MRDIDVRKAVHAKILRDHHKDPDTLIIDEFTMNLGASRADIAVI
                     NGLIHGYELKSKSDNLLRLPAQVQHYSSVMDKVTLVVSDCHLYDALSIVPSWWGIKQV
                     TQGARQGIHLKTIRTSKLNPQVDKLSLTMLLWKDELLSLLSDVGELQNLKNKPKRVLW
                     SKLANSMDVGELREAVRVKLKARKEWRVAQQP"
     gene            complement(3929..4240)
                     /locus_tag="ACHK49_004531"
     CDS             complement(3929..4240)
                     /locus_tag="ACHK49_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265035.1"
                     /translation="MNEIKEIPVVRDEYGCWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGNGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            complement(4233..4487)
                     /locus_tag="ACHK49_004532"
     CDS             complement(4233..4487)
                     /locus_tag="ACHK49_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265036.1"
                     /translation="MSESKCQVNGNKIEPCAALAKSLEHDAEYTMRKGLLIYKIWNES
                     LTRGPDFVMLRSGEFSKLPVRVSFCPFCGESLKTWENRNE"
     gene            complement(4484..4906)
                     /locus_tag="ACHK49_004533"
     CDS             complement(4484..4906)
                     /locus_tag="ACHK49_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO9265037.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLETKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKPKKPSAVDPDLIWSLPDG
                     EIRRYDRRLNIICLECRKSEVMQRVLAFYQGNFEEVVR"
     gene            complement(4947..>5139)
                     /locus_tag="ACHK49_004534"
     CDS             complement(4947..>5139)
                     /locus_tag="ACHK49_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9265038.1"
                     /translation="HRQICEMFGRLQRDSFWVKNIMSPAKLREKWDELVIRLGRSPAQ
                     RCVNHISEPDTEIPPGFRG"
BASE COUNT         1350 a         1217 c         1094 g         1478 t
ORIGIN      
        1 cacgtcatgc atttctgcgt ttttacccaa cgcgtgtata caggcatctc ttcccgtcgt
       61 ggtttctggc gctggagata ctgagccggt gactccggat caacggcaat gcttaccacc
      121 gtcttttcct gtggcgggtt ttgctggtgg gcgtgaggca gtagcgcaat attttttgtg
      181 cgctgcttca gcatgctggt ggcggtctgc tctcccggca cgatgtcgct ctcgcggtat
      241 actgagcgaa ttttttccgc gcgtaatccc agtgaacgac gtaacaccgt ctccggtagt
      301 gcgtccgcta cgttatttat ggttgcccac caggataatt cagccagcga taattcccgc
      361 tcctgcgtgc cattcattgc gtggcggatg acatcaatca tccatgctga caggttttgg
      421 tgagcaagct gcccgagtga ttcggaagtc tggttacgca gctggttgtc gcagtgccag
      481 cacaacacca tcgcgccggt accgtaacga tgtatgacgg tttcactgtg atggtagtca
      541 ccatgaggcc actggcagga tttaatgtgg cgtaacagcc agtcagacaa tgcaccagca
      601 ccaccagcag cacgaatcac ccgctcatcg ctgaaaaatg gcagtaatga tttatcctcc
      661 gccagcggct ggcgaacagc aggaacgact ccggacggca gaccgcgcat gcttttcggt
      721 tccggctcca ccagaactcg agggttatga aatacctgca tggattcacg gcccggtttt
      781 agcaccacca gcccaagttc cggtaccgga acaggtcgaa gtaatacccg cacgttacct
      841 ccagatgcgt tgctggaatg tgcgggacgg acgcggtggg cgttcggaat aagggagcct
      901 gacatagatt atccagtgac gataatcgag gctgagggct ttcttaatct cgtatccgcg
      961 tctgcggtag ttatgaatta gccattcggc ctgttcttca gtacatggtg ggtgttggta
     1021 ccagtcggtt ttaaatgcgt gtgaacgccg cccatgccgg atggcaaggt cggtatcaga
     1081 attgtgaaat ttggttttgt gcaccatctg ttttctctgc tggcgcagca ggtgtcaggt
     1141 gttcaggctg acgtgcgaat tgtaaaccag aatgccagga aaaaacaaaa cccgccgaag
     1201 cgggttaagt gcgggtgcgt tgaggatgcc tgactcatca gaggtggcga gggatttctc
     1261 cctcgcctgg tctcttactc ctcaggttcg taagctgtga agacagcgac ctccgtctgg
     1321 ccggttcgga ttcgtacctc gcagaggtct ttcctcgtta ccagtgccgt cactatgacg
     1381 gttaaacaga tgacgatcag ggcgattaac atcgcctttt gctgcttcat agcctgcttc
     1441 tccttgcctt tcggcacgta agaggctaac ctacatgtgc aaagcatgaa attggcctca
     1501 gattaatgtt aagcgtcttg ccggacgcgt aatgttaact ggggcttttc tctatctgcc
     1561 ttttggtgtt catgcctgag gcagatagcc tcaagcaccc gcagcaattc taactatcca
     1621 atagatcaat gccaacttct tttcctgcaa catctctcat cagaagtgac ccaaattcat
     1681 ccaatcagca caaaagaaac atttaaagcg tgaatgctac cgaacgttca tttcaattac
     1741 atcgttttga tttttaacag ttttcacctc aacaaatcaa atggcattag cattcatcat
     1801 ggaagaaaga ctgataaaaa tgatcatttt caatgactta tgatcacacc gggtattgcc
     1861 aagtcattcc ttttacgcca ggatacccac aagtgagtag tcgaggagag tttgccaatt
     1921 tttgcgatat aaatttcaat gtccgaagaa ccattaattg ttaattgatt ctgtataagg
     1981 tattaacatg acaatttcat atattcctat tctgaaagcg aaacgttctg agttatcagc
     2041 tttatcgcag ctatccattg aaaagaaatc aaaaattttg ccattactcg aaattgaacc
     2101 agtgccaatt gaccctgatt caggtattgc cttaaagagt tataacgaga ctcttataga
     2161 gttcggaaag aaagtctcaa aatcttgctc agatatgcaa ggtgtttata ttgatggatt
     2221 attaattgaa gagcatttta tttctcctga agatcattac cctataataa atgcggttaa
     2281 tcaagttaga gatatggaga taagagttat tcctgtcagt tcaccaactc gcccatctaa
     2341 ctataaaaga gcgattgatg aattaatgca gaatgaaata tgcttgagat taaccacgtt
     2401 agatctggtt aacccacaat taataacgca ttacattaat catctgggaa ttcctttatc
     2461 aaatattgat ataattattg acttaagaga tgagttaacc gaggataaaa ttaattccgg
     2521 cgaactatat actttggcaa tgggattgat aaacaatctg gcgcacctca atgaatacag
     2581 aaaagtgatt ctttctgggg gttcatttcc tacagatctc agtgatattt ctgttggtct
     2641 atattctcaa cctcgaatcg agtggatttt atggcagagt ttaatgaata gaaaagaact
     2701 tgccagaaat gtgatttata gtgattatgg agtacagcac cccgacttta ataggctttc
     2761 gacgcgattc cctagcgtat ctgccagcgt cagatactct ggagataatg acttttgggt
     2821 atttcgagga agagtagcta atcgctttgg ttatgaacaa tatggtaaac atagtgagga
     2881 tattcttgct catcgagaat attcagggcc tacattctgt gcgggagata gagacataga
     2941 atattatgca aacgagtatc aagcctacaa agccaatccc tctggtaact ataaatttgg
     3001 tagcccagag gtatggcgta ggattgggca aaaccatcac ataactaagg ttgttgagca
     3061 actcgccact ctttacgggc tttaagttta actcgaacag cttcacgaag ctcgccaaca
     3121 tccatactat tggcgagttt tgaccataag acgcgtttag gtttattttt caaattctgt
     3181 agctccccta catcacttaa tagggaaagc aattcatctt tccaaagaag cattgttaag
     3241 gaaagtttgt ccacttgtgg attcaacttg ctagttcgaa ttgtttttaa atggatacct
     3301 tgccgtgcac cttgcgtaac ttgctttatc ccccaccatg atggaactat gcttaaagca
     3361 tcataaagat ggcaatcaga gacaaccaaa gttactttat ccatcactga tgagtaatgt
     3421 tgcacctgcg ctggtaatct gagcaagtta tcactcttgc tcttcaactc ataaccatgt
     3481 ataagcccat tgatcactgc tatatcagct ctgctagccc ctagattcat cgtaaactca
     3541 tcaatgatta gggtgtcagg atctttatga tgatctctca gaatcttggc atgcacagcc
     3601 tttcttacat caatgtctct cataccatag ttgcgctttc atcgactctc tcaagccatg
     3661 attctatatc atggttcgca tgaatgccat ttgatatgac cagtggttaa acattgcaca
     3721 gaaaagtgga tgtgtatttt aacaaaagca atgacggcta accatgttgg tatgtcgata
     3781 aaatgtttca acgtaagcat tatctgttcc gccctttcag acggcctcct gatgttctga
     3841 gggtgcagaa tccctccggt taagggttta ataaaaatcg tttctgattt aaatcttcag
     3901 tatttagttg ttagtcggtt tatagccttt atgcttcggc cttatttctc agccatacac
     3961 aaaccgggcc atcttcggtg tcatgtattg aaccaataaa ccatccattg ccctctggtc
     4021 gttccggttc ccatgcagaa atatcagcat cacacgcatc aaggtcagca catccttcat
     4081 ctctgaagca gaggacgtat tgaagattat tttcctccat ccaggcgtta aactcttccg
     4141 ttgaaatata ttcccgaccg tcacagaatt tttcatattc aggatgcgtc cagcagccat
     4201 attcatcacg tactactggt atttctttaa tttcattcat ttctgttctc ccacgttttc
     4261 agactttcac cacagaacgg acaaaatgaa acccgaactg gtaatttaga aaattcaccg
     4321 gaacgcaaca tcacaaaatc aggaccgcga gttaaactct cattccagat tttgtatatc
     4381 agcagacctt ttcgcatcgt gtattcagca tcatgctcaa gggactttgc cagtgctgca
     4441 catggttcta tcttgttgcc attaacctgg cattttgatt cactcaccgc accacctcct
     4501 caaaattccc ctgataaaac gccagtacgc gctgcataac ttcgctcttc cggcactcga
     4561 gacagattat gttcagacgc ctgtcgtagc ggcgtatttc gccatctggt aatgaccaga
     4621 taaggtccgg atcaaccgca gatggtttct tcggctttgc ccttgagagc tttttacggg
     4681 cattttgcca gtccttacgc gcctgttcag acgggaataa cccgtaacca gagttgtata
     4741 catcgccact ggcaaccagc tctctggcca gaacgctcat cagatatctt gttgccccag
     4801 ttttagtttc cagttgtcgt aacgtctcgc gcccactctg gcgtacgagt tcaacaacct
     4861 gccctttaat tttttcccgc tcttcttgtg taaaaacttt tgccacaagc cctcctgaaa
     4921 attacctcat gaccagaaat taacacttac cccctgaagc ccggcggaat ttcagtgtcc
     4981 ggttcagaaa tgtgattcac gcaacgctgc gcaggcgaac gccccaggcg gataaccagt
     5041 tcatcccatt tttcccggag ttttgccgga ctcatgatgt tttttaccca gaacgaatcc
     5101 cgctggagac gcccaaacat ttcacaaatt tgtctgtga
//
LOCUS       DAZGLJ010000283         5136 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000283 DAZGLJ010000000
VERSION     DAZGLJ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.283"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1967
                     /gene="acnA"
                     /locus_tag="ACHK49_004535"
     CDS             <1..1967
                     /gene="acnA"
                     /locus_tag="ACHK49_004535"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO9265039.1"
                     /translation="LGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVV
                     GKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVEL
                     VEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASN
                     ELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAV
                     TLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPD
                     PIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAAE
                     PIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSD
                     TYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDS
                     PAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDS
                     DVVSIYDAAMRYKKEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHR
                     SNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVV
                     PCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(2031..2621)
                     /gene="ribA"
                     /locus_tag="ACHK49_004536"
     CDS             complement(2031..2621)
                     /gene="ribA"
                     /locus_tag="ACHK49_004536"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO9265040.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            2791..3555
                     /gene="pgpB"
                     /locus_tag="ACHK49_004537"
     CDS             2791..3555
                     /gene="pgpB"
                     /locus_tag="ACHK49_004537"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO9265041.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            3704..4012
                     /gene="lapA"
                     /locus_tag="ACHK49_004538"
     CDS             3704..4012
                     /gene="lapA"
                     /locus_tag="ACHK49_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO9265042.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            4019..>5136
                     /gene="lapB"
                     /locus_tag="ACHK49_004539"
     CDS             4019..>5136
                     /gene="lapB"
                     /locus_tag="ACHK49_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO9265043.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPS"
BASE COUNT         1258 a         1214 c         1396 g         1268 t
ORIGIN      
        1 tgttaggcca gccggtttcc atgcttatcc cggacgtagt gggctttaaa cttaccggta
       61 aattacgtga aggtattacc gccacagacc tggttctcac tgttacccaa atgctgcgca
      121 aacatggcgt ggtggggaaa ttcgtcgaat tttatggtga tggtctggat tcactaccgc
      181 tggcggatcg cgccaccatt gccaatatgt cgccagaata tggtgccacc tgtggcttct
      241 tcccaatcga tgctgtaacc ctcgattaca tgcgtttaag cgggcgcagc gaagatcagg
      301 ttgagttggt cgaaaaatat gccaaagcgc agggcatgtg gcgtaacccg ggcgatgaac
      361 cgatttttac cagtacgtta gaactggata tgaatgacgt tgaagcgagc ctggcagggc
      421 caaaacgccc acaggatcgc gttgcactgc ccgatgtacc aaaagcattt gccgccagta
      481 acgaactgga agtgaatgcc acgcataaag atcgccagcc ggtcgattat gttatgaacg
      541 gacatcagta tcagttacct gatggcgctg tggtcattgc tgcgataacc tcgtgcacca
      601 acacctctaa cccaagtgtg ctgatggccg caggcttgct ggcgaaaaaa gccgtaactc
      661 tgggcctcaa gcggcaaccg tgggtcaaag cgtcgctggc accgggttcg aaagtcgttt
      721 ctgattatct ggcaaaagcg aaactgacac cgtatctcga cgaactgggg tttaaccttg
      781 tgggatacgg ttgtaccacc tgtattggta actctgggcc gctgcccgat cctatcgaaa
      841 cggcaatcaa aaaaggcgat ttaaccgtcg gtgcggtgct gtccggcaac cgtaactttg
      901 aaggccgtat ccatccgctg gttaaaacta actggctggc ctcgccgccg ctggtggttg
      961 cctatgcgct ggcgggaaat atgaatatca acctggctgc tgagcctatt ggtcatgatc
     1021 gtaaaggcga cccggtttat ctgaaagata tctggccatc ggcacaagaa attgcccgtg
     1081 cggtagaaca agtctccaca gaaatgttcc gcaaagagta cgcagaagtt tttgaaggca
     1141 cagcagagtg gaaggaaatt aacgtcacac gatccgatac ctacggttgg caggaggact
     1201 caacctatat tcgcttatcg cctttctttg atgaaatgca ggcaacacca gcaccagtgg
     1261 aagatattca cggtgcgcgg atcctcgcaa tgctggggga ttcagtcacc actgaccata
     1321 tctctccggc gggcagtatt aagcccgaca gcccagcggg tcgatatcta caaggtcggg
     1381 gtgttgagcg aaaagacttt aactcctacg gttcgcggcg tggtaaccat gaagtgatga
     1441 tgcgcggcac cttcgccaat attcgcatcc gtaatgaaat ggtgcctggc gttgaagggg
     1501 ggatgacgcg gcatttacct gacagcgacg tagtctctat ttatgatgct gcgatgcgct
     1561 ataagaagga acaaacgccg ctggcggtga ttgccgggaa agagtatgga tcaggctcaa
     1621 gccgtgactg ggcggcaaaa ggtcctcgtc tgcttggaat tcgtgtggtg attgccgaat
     1681 cgtttgaacg aattcaccgt tcgaatttaa ttggcatggg catcctgccg ctggaatttc
     1741 cgcaaggcgt aacgcgtaaa acgttaggac taaccgggga agagaagatt gatattggcg
     1801 atctgcaaaa tctacaaccc ggtgcgacgg ttccggtgac gcttacgcgc gcggatggta
     1861 gccaggaggt cgtaccctgc cgttgtcgta tcgacaccgc gacggagttg acctactacc
     1921 agaacgacgg cattttgcat tatgtcattc gtaatatgtt gaagtaacaa gtatttgctt
     1981 gccggttatt ttgcttccgg caagcaaatt aattacacaa tgcaagaggg ttatttgttc
     2041 agcaaatggc ccattttctc ggctttggta tcgagataat gttcgttatt ggggttacga
     2101 cctacaatca atggtacgcg ttcaacaata ttaatccctg cttcggtcag aatttcgact
     2161 tttttcgggt tattggttaa caagcggact tcattgacgc caaggagttt gaacatatca
     2221 gcgcaaagag tgaagtcgcg ctcatcagcg gcgaagccta actggtggtt agcctctacg
     2281 gtatcgtaac cttgatcctg cagtgcgtaa gcgcggattt tattcagcag accaatgtta
     2341 cgaccttcct gacggtgata cagcaaaata ccacggcctt cctcggcaat ttgcgtcaat
     2401 gccgcttcga gctggaagcc acaatcgcag cgcaagctga acagggcgtc accggtcaga
     2461 cattcggaat ggacgcgcgc aagtaccggg gtatgcccgg aaatatcgcc atagactagc
     2521 gcgacatgat cgtgtccggt tgccagttct tcaaatccca ccatcaggaa atcgccccat
     2581 ggggttggca gtttggcttc tgccacacgt ttaagctgca tgaaattctc cagataatgc
     2641 tggttctgta ttggcttatt ttgccataac gagaaggggt tcacctaatc acgacgcgtc
     2701 gatcgttcac ggaatggcac aaatctgtca atttttcctg gaactggcgt tttcagttat
     2761 gattgtggga cttatcaaaa aggagaggcc atgcgttcga ttgccagacg taccgcagtg
     2821 ggagctgcac tattgcttgt catgccagta gccgtatgga tttctggctg gcgttggcaa
     2881 cctggagaac aaagttggct actaaaagcg gctttttggg ttactgaaac tgtcacccag
     2941 ccctggggcg tcattacaca tttgatttta ttcggctggt ttctctggtg tctgcgtttt
     3001 cgcattaagg ctgcctttgt attatttgcc attctggcgg ccgcaatcct tgtgggacaa
     3061 ggcgttaaat cctggatcaa agacaaagtc caggaaccac gaccttttgt tatctggctg
     3121 gaaaaaacac atcatattcc ggttgatgag ttctacactt taaagcgagc agaacgcgga
     3181 aatctagtga aagaacagtt ggctgaagag aaaaatatcc cacaatattt gcgttcacat
     3241 tggcagaaag agacggggtt tgcctttcct tccggtcaca cgatgtttgc tgccagttgg
     3301 gcactgctgg ccgttggttt gctgtggccg cgtcggcgaa cgttaaccat tgctatcttg
     3361 ctggtctggg caacgggagt catgggaagc cgcctgctgc tcgggatgca ttggccacgc
     3421 gatctggtag tagctacgtt gatttcgtgg gcgctggtgg cggtggcaac gtggcttgcg
     3481 caacgaattt gtgggccatt aacaccacct gcggaagaaa atcgcgaaat agcgcaacga
     3541 gaacaagaaa gttaacgctg gttgattttc cgaatttagc ccttaaatca tcaacaatgc
     3601 gcgtggatgc catttcgcag acggcgcgaa aatggtactt taaagggcta ttgcggtaag
     3661 ttgaccataa tttattcgct ctaaccacat aacgggaagt aatgtgaaat atttactcat
     3721 tttcttactg gtgttagcga tcttcgtgat ttcggtcacg ttgggtgcgc agaacgatca
     3781 acaggtgacg tttaattatc tgttagcgca aggggagtac cgtatttcca cattgctggc
     3841 ggtattgttt gctgcggggt ttgctatcgg ttggttgatt tgtggcctgt tctggctgcg
     3901 agttcgtgtt tccctggcgc gcgctgaacg taaaataaag cgactggaaa accagctttc
     3961 acccgcgact gacgtggctg tagtgccgca ctcgtcagcg gcgaaggaat aactttctat
     4021 gctggagttg ttgtttctgc ttttgcctgt agccgcggcc tatggctggt atatgggccg
     4081 cagaagtgcg caacaaaaca agcaagatga agccaaccgc ttgtcgcgtg attacgtagc
     4141 gggggttaac ttcctgctta gtaatcaaca ggataaagcg gtagacctgt ttctcgatat
     4201 gcttaaagag gatacaggca ccgttgaagc ccaccttacg ctcggaaacc tgttccgttc
     4261 gcgtggcgaa gttgatcgcg ctattcgcat ccatcagacc ctaatggaaa gcgcctcgct
     4321 gacctatgaa cagcgtctgt tggcgattca acaactaggg cgtgattaca tggccgccgg
     4381 gttatacgac cgcgcggaag acatgttcaa tcagctgacc gatgaaactg acttccgcat
     4441 tggcgctctg caacagttgc tacaaatcta ccaggctacc agtgagtggc agaaagcaat
     4501 tgatgttgcc gaacgcctgg tgaagctggg taaagataaa cagcgcgtcg aaattgccca
     4561 tttctactgt gagttagccc tgcagcatat ggccagcgac gatctcgatc gtgccatgac
     4621 cttgctaaaa aaaggggcgg cggcagataa aaacagcgcc cgcgtatcca tcatgatggg
     4681 acgcgtgttt atggcgaaag gagaatacgc caaagccgtc gaaagtttgc aacgcgtgat
     4741 atcccaggac agagaactgg tcagcgaaac gctggaaatg ttgcaaacct gctatcagca
     4801 gttgggtaaa actgccgaat gggcagaatt cctgcaacgc gcggtggaag agaacaccgg
     4861 tgccgatgct gaattgatgc ttgctgatat catcgaagcg cgcgacggta gtgaggccgc
     4921 acaggtctat attacgcgcc agcttcagcg tcatccgacc atgcgtgtgt tccataagtt
     4981 aatggattac cacttaaatg aagcggaaga agggcgtgcc aaagagagcc tgatggtgct
     5041 gcgtgacatg gttggcgaaa aggtacgtag taagcctcgt tatcgctgcc agaaatgtgg
     5101 ttttaccgca tacaccctct actggcattg tccgtc
//
LOCUS       DAZGLJ010000284         4970 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000284 DAZGLJ010000000
VERSION     DAZGLJ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.284"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(116..874)
                     /gene="chbG"
                     /locus_tag="ACHK49_004540"
     CDS             complement(116..874)
                     /gene="chbG"
                     /locus_tag="ACHK49_004540"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO9265044.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(887..2239)
                     /gene="celF"
                     /locus_tag="ACHK49_004541"
                     /gene_synonym="chbF"
     CDS             complement(887..2239)
                     /gene="celF"
                     /locus_tag="ACHK49_004541"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO9265045.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(2344..3186)
                     /gene="chbR"
                     /locus_tag="ACHK49_004542"
     CDS             complement(2344..3186)
                     /gene="chbR"
                     /locus_tag="ACHK49_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO9265046.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(3194..3544)
                     /gene="chbA"
                     /locus_tag="ACHK49_004543"
     CDS             complement(3194..3544)
                     /gene="chbA"
                     /locus_tag="ACHK49_004543"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO9265047.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEVKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(3595..4954)
                     /gene="chbC"
                     /locus_tag="ACHK49_004544"
                     /pseudo
     CDS             complement(3595..4954)
                     /gene="chbC"
                     /locus_tag="ACHK49_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
BASE COUNT         1359 a         1310 c         1056 g         1245 t
ORIGIN      
        1 agcgcatcag gcagttttgc gtttgtcagc agtctcaagc ggcgcagtta cgccgccttt
       61 gtaggaatta atcgccggat gcaaggttca cgccgcatct ggcaaacatc ctcacttaca
      121 catcccgata actccccaac cgataaccac gctgagcgat agcacctttc aacgacgctg
      181 atgtcaacac atccagctcc gttaagcgtg ggaaacagta agcactctga cggatagtat
      241 tatcgataaa cgccggatga cacatcacct ccagcgaacg atcaccccga tggcttgcgt
      301 catccagcac ttgcaggaac agtgcctcgc taatctcttc accataaaac gcgctactga
      361 atccctgaga actgcgcaga ttagccggta aatcaccagc gttagatagc ggctgacggt
      421 cgatgcgcaa cgcaatcccc tcttcagccg caaacctggc aacaatcggg aaaatctgcg
      481 ggaacatatg cacatgatga tggctatcaa gatgcgtagg tttgcgtcca aatagctcaa
      541 tgaaacgcaa atactgactg gcaagctcct gagtaatttc ttccagcggt aaagcatctt
      601 cttctgccaa ctgccagatc cattttccca gcacaccatc gcgggttaac cccggcatag
      661 ctgtcagggg cttgcccata gtaaggacaa agtgcatccc tatggccaga ctcggttcat
      721 cacgactcaa ctgcaccgca tggtcaatag cctgcccatt caccagcgcc gtcgttgatg
      781 tgacaatccc attgcgacag gcctcgataa tgccgtagtt ctgacctttg cttaagccaa
      841 aatcatcggc attaacaatc agtaagcgtt ccataatcag cctcggttaa tgtgcttttt
      901 taagctctgc gatgcagtcg gcaaaatttg gcagccattt ctcgtgcgcc agaatcatct
      961 cgcgtgccag cagctcagca tcgcgatcgg aatgcaccaa cggactaagg tttagcgcca
     1021 gtaacacatc gttaaattct ccgctaagtg cggcgttgct ggcagcaatc tcgaagcctt
     1081 taatggtgtg aatcagcccc atcactttat catcgaaatg cgtaatgcgc ggatgtggcg
     1141 tcgcgccatc gcgccccagc ttacaggtca tttctaccgc ccagtctgcc ggaatattat
     1201 caatatgccc atgatgcggg atattaacgt aatgttcagc ttgcttgtcg ttgtagatag
     1261 cgttgatcac ttcgcacgct gcatcagagt aataagcccc accgcgctgt tccagttctt
     1321 tcggcttaac tttcaactcc ggatttttat acagctcaaa aagttgtttc tcgactttct
     1381 gtactacctg tgctcgtgcg ccgcctttgt agtattcgcc catttcaata gccagcatct
     1441 ctttctgctt gaagtaatac agcagataag aacatggcag cagatttaac gaacgaatta
     1501 agccctcact aaatggcaga tcgaaaatat tttttacaga ggacgctttt aactgccctg
     1561 acgccacacc atcaagcaat tcggcaaagc gcgacttgcc atttaccagc acatccttaa
     1621 tgaacaccat atggttgagg ccgaacagat cgatagataa atcatcgctg tctttcagca
     1681 tcagaacatc gcgaataaac atcttcatgc cgatcggaat attacacacg ccgataaagc
     1741 gtttaaatcc ggtatgacga taaacggctt cagtgaccat tcccgccggg ttagtgaagt
     1801 taatcaccca tgcattcgga caaagttctt cgacatcttt tacgatgtca aaaatcaccg
     1861 gaatggtacg cagaccttta aacagaccgc ccgcgccgtt ggtttcctga ccaagataac
     1921 catgacttaa tggaatacgt tcatccagtt cgcgcgccgg taattggcct acgcgcagtt
     1981 gggtagtaac gaaatcagca tctttcaatg cttcgcggcg atccagcgtt ttataaagct
     2041 tcatcgggac gccagcgtta tcaatcatcc gttggcagag ttcaaaaatg atatccagtt
     2101 tctctttacc atcttcgaca tccaccagcc ataattcgct gaccggcaat tcgtgataac
     2161 gcttaataaa tccttccagt aactccgggg tatagctgct cccgccacca atagtgacga
     2221 cttttaattt ctggctcata atttctccct tcagtacaga atactgatat ctggcatatc
     2281 tgcccccccg gacataaata atccagcaac aggacagata tgtgaattgt caggtataac
     2341 gacttactga ttaaattcag tcaatttctt acgatagctc ttaggcgtaa aggacgttaa
     2401 tttcttaaac gttttaataa acaaactcgg gctactataa ccggcctcaa acgcaatatc
     2461 cgtcacggaa tagttggtca tttccagttg ttttttggca aaattaatac ggatctcatt
     2521 aataatctgc attggtgttt tgccataata tcgttgagtg gctcgcgtca aatattcctg
     2581 tgatttggct gacaacgtca ccatattctc cagcgccgat tcactaaact gctctttatc
     2641 atgcatcttt tctaccgtac ttttcagcca ctgcggtaca tcatcaatca cctgttcttc
     2701 gcggtaatga cgtaaacggt taatgacata aaaagtaacc atctcaacaa actcttccag
     2761 ccctgtttcg cggaaattca atgaagaaat cactgtttcc acataggtga gaaaagcgtt
     2821 attggtccgg tatacctgcg aagcgacaaa gcaataaggc aacaacggca ggtaatgctg
     2881 ctcaaaaaag cgtttactga tcccaacgtt caatatacgc gtggcaccaa actcataaaa
     2941 actttgatgg tgcgaaccta acggaataaa aacaaaatcg ccccgttcca gtaacacgcg
     3001 cttaccgtta atctcctgga aataacgccc ggttaatacc agagtaaatt cataatagtc
     3061 gtgctgatgc agcccgctga tactctcagt tttgttatag ataaacacat ggaagttttt
     3121 gccattaaac aactgctgtt ctcgggcagt ggcaatttcc ggcgcgttaa tcactggctg
     3181 catcatcgac tccttatgcc ttcagttttt catgaagctc aattaattca gtaatcagtt
     3241 cacgcgcaag catggacgtc attaaatgat cctgagcgtg gaccagcacc agactcactt
     3301 tcatcttaac ttcgcccgca tcgccttcaa tcagtttcgt ctgtaccaga tgcgcttcat
     3361 tcaatgccat ccgtgactga tccatcatgg cttttgctgc agcaaaatcg ccctgcttcg
     3421 cctgtttcag tgccgcatac gccaggctgc gcgcttgtcc ggagttgatg ataagcccca
     3481 tcaccacttc ttccagctct tcagcttctg tttgcgtatc aggaatatta tcgagatcca
     3541 tcatacatcg ttcctctctc ttcttaccgg caagattacc cgtaccggca tcgattaaaa
     3601 tttcagggcg ttagcgatat cttcttcgct ctcttcttta tcaatcgcat tttgtgcttt
     3661 gttagccacc acaacaaagg gcagataaat taaggttgcg atgccaaggt tgaagagtgc
     3721 gaccagcaat gcggcgacgc taccgttggt gttaaagaag gctcccagac cggttggcat
     3781 ggtccacggt gcaatattgg tcaccggagg aatgatgccc atgtagtacg ctgcgagggt
     3841 gattgccgcc agaatcggtt gtaccagtac aaacgggata aacatcaccg ggttcatgat
     3901 aattggcaga ccaaacagaa tcggttcgtt aatctggaag atgccggacg gcagcgccag
     3961 ttttgccacc tgacgataat cagcacggcg agaggcgata aagatagcca ggatcaggcc
     4021 taaagtcgca ccactgcccc caaggaaaat aaaggagtcc agcatcggct tggcccagat
     4081 atggaaggtc ttaccggctg ccagcgccgc ttcgacggaa ccatattgct gataggtcgc
     4141 gatattttcc agcgcccacg gcgtcataat gccgttgtcc agtgcggtca gcgccagcgc
     4201 gccatgaata ccgaagaacc agagcagtgg aacaaagatc acataggccc agcccaccac
     4261 gctacccaac gatgccagtg gggttgagat ggtatccata atgatctgat ggaagttggt
     4321 gccccaggta ttcaacgccc aggcaataat ccccatcacg gaaagaataa taaagccggg
     4381 aattaatgcc gagaaggaac gcgatactga agcaggtacg ctgtcgggca gtttaatgac
     4441 ccaattgcgg cggacaataa aggtaaacat ttctgccacc accaggccaa taataatccc
     4501 ggagatgata ttcgccccac ctaaccagtt tgcgccaacc gcataggcct caccgacact
     4561 atatgggggt gacggtcata aatgctgcaa cggataacaa ccccgccgcc agcgcatcga
     4621 ctttacgctc ttctgccagc gccatgccaa taaagaacgg tgccattaaa gacattatcc
     4681 ctaatgttcc gttatatacg ttgccgccaa tacctttcag gccattaagt gtttcaatgg
     4741 ttgaggcgtc gaggcgaata cctaaggaat aaaaaaacga cccctcccca aagcttagaa
     4801 aaacgttgtt aattaataca aacatggccc ccgcaagggt taacggcatt aagcgaataa
     4861 agccattttt gattgcatta acgtgtggct gctttcctat tttaactgca aaagggagga
     4921 gtaccttttc aagcgatgca ataacattac tcatagaaaa atacccttaa
//
LOCUS       DAZGLJ010000285         4934 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000285 DAZGLJ010000000
VERSION     DAZGLJ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.285"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..683
                     /gene="bet"
                     /locus_tag="ACHK49_004545"
     CDS             <1..683
                     /gene="bet"
                     /locus_tag="ACHK49_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO9265048.1"
                     /translation="KGDASDAQFIALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGV
                     DGWSRIINENQQFDGMDFEQDNESCTCRIYRKDRNHPICVTEWMDECRREPFKTREGR
                     EITGPWQSHPKRMLRHKAMIQCARLAFGFAGIYDKDEAERIVENTAYTAERQPERDIT
                     PVNDETMQEINTLLIALDKTWDDDLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQK
                     ATEQKVAA"
     gene            680..1360
                     /locus_tag="ACHK49_004546"
     CDS             680..1360
                     /locus_tag="ACHK49_004546"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO9265049.1"
                     /translation="MIPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            1357..1539
                     /locus_tag="ACHK49_004547"
     CDS             1357..1539
                     /locus_tag="ACHK49_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO9265050.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            1512..1703
                     /locus_tag="ACHK49_004548"
     CDS             1512..1703
                     /locus_tag="ACHK49_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO9265051.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            1780..1995
                     /locus_tag="ACHK49_004549"
     CDS             1780..1995
                     /locus_tag="ACHK49_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO9265052.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQHFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            2094..2312
                     /locus_tag="ACHK49_004550"
     CDS             2094..2312
                     /locus_tag="ACHK49_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO9265053.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRQNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
     gene            2360..2638
                     /locus_tag="ACHK49_004551"
     CDS             2360..2638
                     /locus_tag="ACHK49_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9265054.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            2610..2675
                     /locus_tag="ACHK49_004552"
                     /pseudo
     CDS             2610..2675
                     /locus_tag="ACHK49_004552"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2837..4000
                     /gene="intD"
                     /locus_tag="ACHK49_004553"
     CDS             2837..4000
                     /gene="intD"
                     /locus_tag="ACHK49_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO9265055.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(4016..4092)
                     /locus_tag="ACHK49_004554"
     tRNA            complement(4016..4092)
                     /locus_tag="ACHK49_004554"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4056..4058),aa:Arg,seq:tct)
     gene            4335..>4934
                     /gene="fimZ"
                     /locus_tag="ACHK49_004555"
     CDS             4335..>4934
                     /gene="fimZ"
                     /locus_tag="ACHK49_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO9265056.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVEL"
BASE COUNT         1463 a         1116 c         1212 g         1143 t
ORIGIN      
        1 ttaaaggtga tgccagcgat gcgcagttca tcgcattgtt gatcgtcgcc aaccagtacg
       61 gccttaatcc gtggacgaaa gaaatttacg ccttccctga taagcagaac ggcattgttc
      121 cggtggtggg cgttgatggc tggtcccgca tcatcaatga aaaccagcag tttgatggca
      181 tggactttga gcaggacaat gaatcctgta catgccggat ttaccgcaag gaccgtaatc
      241 atccgatctg cgttaccgaa tggatggatg aatgccgccg cgaaccattc aaaacccgcg
      301 aaggcagaga aatcacgggg ccgtggcagt cgcatcccaa acggatgtta cggcataaag
      361 ccatgattca gtgtgcccgt ctggccttcg gatttgctgg tatctatgac aaggatgaag
      421 ccgagcgcat tgtcgaaaat actgcataca ctgcagaacg tcagccagaa cgcgacatca
      481 ctccggttaa cgatgaaacc atgcaggaga ttaacactct gctgatcgcc ctggataaaa
      541 catgggatga cgacttattg ccgctctgtt cccagatatt tcgccgcgac attcgcgcat
      601 cgtcagaact gacacaggcc gaagcagtga aagctcttgg attcctgaaa cagaaagcca
      661 ctgagcagaa ggtggcagca tgataccgga cattatcctg cagcgtaccg ggatcgacgt
      721 gagagctgtc gaacaggggg atgatgcatg gcacaaatta cggctcggcg tcatcaccgc
      781 ttcagaagtt cacaacgtga tagcaaagcc ccgctcagga aagaagtggc ctgacatgaa
      841 aatgtcctac ttccacaccc tgctggctga ggtttgcacc ggtgtggctc cggaagttaa
      901 tgctaaggcg ctggcctggg gaaaacagta cgagaacgac gccagaaccc tgtttgaatt
      961 cacttccggc gtgaatatta ctgaatcccc gatcatctat cgcgacgaaa gtatgcgcac
     1021 cgcctgctct cccgatggtt tatgcagtga cggcaacggc cttgaactga aatgcccgtt
     1081 tacctcccgg gatttcatga aattccggct cggtggtttc gaggcaataa aatcggctta
     1141 catggcccag gtgcagtaca gcatgtgggt gacgcgaaaa gatgcctggt actttgccaa
     1201 ctatgacccg cgcatgaagc gtgaaggcct gcattatgtc gtgattgagc ggaatgaaaa
     1261 gtacatggcg agttttgacg agatggtgcc ggagttcatc gaaaaaatgg acgaggcact
     1321 ggctgaaatt ggttttgtat ttggggagca atggcgatga cgcatcctca cgataatatc
     1381 cgggtaggcg cgatcacttt cgtctactcc gttacaaagc gaggctgggt atttcccggc
     1441 ctttctgtta tcagaaatcc actgaaagca cagcggctgg ctgagaagat aaataataaa
     1501 cgggaggcgg tatgcacaaa gcatctcctg ttgagttaag aacgagtatt gagatggcac
     1561 atagccttgc tcaaattgga gtcaggtttg tgccaatacc agtagaaaca gacgaagaat
     1621 ttcatacgtt agccacatcc ctttcacaaa agctggaaat gatggtggcg aaagcagaag
     1681 cagatgagag agaccaggta tgacaaccac tgaatgcatt tttctggcag cgggcttcat
     1741 attctgtgtg cttatgcttg ccgacatggg gcttgttcaa tgacacctca gcaagaaaac
     1801 gcccttcgca gcattgcccg tcaggctaat tctgaaatca aaaaagccag acagcatttt
     1861 ccggataaaa acgtcgatga catttgccgt agcgtactaa agaagcaccg cgaaacggta
     1921 acgctgatgg gattcacacc gactcattta agcctggcga tcggcatgtt aaacggcgtc
     1981 tttaaggaac ggtgaacatg aaaagcaaaa tcatcaggga gctacaggct ccttttttat
     2041 tgttcgcatt catcctcaag cgtattaacc aacaattcag ggattaatgg aagatggcag
     2101 acatcattga ttcagcatca gaaatcgaag aattacagcg caatacagca ataaaaatgc
     2161 gtcgtcagaa ctaccagact gtatccgcaa ctcattgttg tgagtgtggc gatccgatag
     2221 atgagcgaag acgcctggct gttcagggtt gtcggacttg tgcaagttgc caggaggaga
     2281 tcgaacttaa gaacaaacaa tggggactgt gatggcctca aagcagcaaa tttcaacatc
     2341 gtccaactga ggtgtaaaaa tgttcagaat catttttcct aacacctggt acgtcgacca
     2401 ccacggcact ccctgcaaaa tcctgcgttc tacccacaac aaagttcact acatccgaaa
     2461 aggcagaaca tgtatcgcca gcatgttccg ctttaatcat gactttgaac ctgtgaataa
     2521 agctgatgca gatcggatag cagaagagat cgaaacggca gaacacatta agaagttacg
     2581 tgacatgcgt tcaaaaagca gaggtaacca tggaatcata cagcctcaca ctcgatgagg
     2641 cctgtcagtt tcttaagata tccagaccaa ccgccaccaa ctggatacga acaggccgcc
     2701 tacaggcaac acgtaaagat ccaaccaagc caaaatctcc ttacctcaca acacggcaag
     2761 cctgcattgc ggcgcttcag tctccgctgc atactgtcca ggtgagcgcg ggtgatggca
     2821 taacagagga aagaaaatgt cactcttccg cagaaatgaa atatggtatg cctcgtattc
     2881 gctcccgggc gggaaacgaa ttaaggaatc tcttggcaca aaggacaagc ggcaagctca
     2941 ggagttgcac gacaagcgaa aagcagaact ctggcgagta gaaaagctag gggatttacc
     3001 tgatgtcact tttgaagagg cctgcctaag atggcttgag gaaaaagctg ataaaaaatc
     3061 tctcgattca gataaaagcc ggattgagtt ctggcttgaa cattttgagg gtataaggct
     3121 taaagatatc tcggaggcaa agatttactc tgctgtaagc agaatgcata acagaaagac
     3181 gaaagaaata tggaaacaga aagttcaggc cgccatcagg aaaggtaaag aaccgcctgt
     3241 ttatgaacca aagccagtat caactcagac aaaggcaaag catcttgcca tgataaaggc
     3301 cattctccgt gctgcagaac gcgactggaa gtggctggaa aaagcgcctg tcatcaagat
     3361 accagcggtc agaaacaagc gagtcagatg gctggaaaag gaggaagcaa aacgccttat
     3421 tgatgagtgc cccgaaccac tgaaatctgt cgtcaagttt gcgctggcaa ctggtctgag
     3481 aaagtcgaac atcataaatc tggaatggca acaaatcgac atgcagcgac gagttgcctg
     3541 ggtgaatcca gaagagagca aatcaaaccg cgccattggt gtggcgctga acgataccgc
     3601 ctgtaaagtg ttgcgtgatc aaataggcaa gcatcacaaa tgggtgtttg tacataccaa
     3661 ggcggctaag cgagcagatg gaacatcaac gcctgcggtc aggaagatgc gcatcgacag
     3721 caagacatca tggctatcag cttgtcgtcg tgcaggaatt gaagatttcc gtttccatga
     3781 cctcagacac acctgggcaa gctggctgat tcagtcaggc gtcccattat cagtgcttca
     3841 ggaaatgggc ggatgggagt ccatagaaat ggttcgtagg tatgctcacc ttgcgcctaa
     3901 tcatttgaca gagcatgcga ggaaaataga cgacattttt ggtgataatg tcccaaatat
     3961 gtcccactct ggaattatgg aggatataaa gaaggcgtaa ctgattgaat tgtaatggcg
     4021 cgccctgcag gattcgaacc tgcggcccac gacttagaag gtcgttgctc tatccaactg
     4081 agctaagggc gcgttgatac cgcaatgcgg tgtaatcgcg tgaattatac ggtcaaccct
     4141 tgctgagtca atggcttttg atctggttgc tgaacaagtg aacgaccgcg tctgattttc
     4201 tgatttattt cgctatagcg gcaaacaaac acacaccgct gcgcgtctga atcaagaaaa
     4261 cccgtatttt catgtatcaa agtacaattt accgccctaa cggaaaattg tccgctccta
     4321 tgagactggt aactatgaaa ccaacgtcgg tgatcattat ggatactcat cctatcatca
     4381 gaatgtctat tgaagttctg ttgcaaaaaa acagtgaatt gcagattgtc ctgaaaacgg
     4441 atgattaccg cataaccatc gattatctcc gaacccgtcc tgttgattta atcattatgg
     4501 atatagactt gcccggaaca gacggtttta ccttcctgaa aaggatcaaa caaatccaga
     4561 gcacagtgaa agtgttattt ttatcatcga aatcagaatg cttttatgct ggcagagcga
     4621 tacaagctgg tgctaacggt tttgtcagta aatgcaatga tcagaatgat atttttcatg
     4681 ccgttcagat gatcctctcc ggatacacgt tttttcccag cgaaacgctt aactatataa
     4741 aaagcaataa atgtagtacg aatagttcaa cggtcactgt gctatctaat cgtgaagtga
     4801 ccatattacg ttatctggtt agcggattat ctaataaaga aattgccgat aagttattac
     4861 ttagcaataa aacagttagt gcgcataaat ctaatattta tggcaagcta ggtttgcatt
     4921 caattgtaga gctt
//
LOCUS       DAZGLJ010000286         4847 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000286 DAZGLJ010000000
VERSION     DAZGLJ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.286"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..411
                     /locus_tag="ACHK49_004556"
     CDS             <1..411
                     /locus_tag="ACHK49_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO9265057.1"
                     /translation="NAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSAK
                     HSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARI
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            522..2906
                     /locus_tag="ACHK49_004557"
     CDS             522..2906
                     /locus_tag="ACHK49_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFO9265058.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRRAALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTSDLGW
                     ENYDEDVSRSVININKAREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCAFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            2903..3808
                     /locus_tag="ACHK49_004558"
     CDS             2903..3808
                     /locus_tag="ACHK49_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO9265059.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            3805..4847
                     /locus_tag="ACHK49_004559"
                     /pseudo
     CDS             3805..4847
                     /locus_tag="ACHK49_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
BASE COUNT         1235 a         1183 c         1402 g         1027 t
ORIGIN      
        1 aacgccggtt atgtgaagtt cgggcatggc agtgcacaac atgtgcgtgc cggtttccgt
       61 ctgggcagcc acaacgatat gacctttggt gaaggcacct catcccgtga caccctgcgc
      121 gacagtgcaa aacacagtgt gagtgaactg ccggtgaact ggtgggtaca gccttctgtt
      181 atccgcacct tcagctcccg gggtgacatg agcatgggga cagccgcagc cggcagtaac
      241 atgacgttct caccgtcccg gaatggcacg tcactggacc tgcaggccgg actggaagcc
      301 cgtatccggg aaaatatcac cctgggcgtt caggccggtt atgcccacag cgtcagcggc
      361 agcagcgctg aaggctataa cggtcaggct acgctgaata tgactttctg attatacggt
      421 atcccggaaa tacgatttca ggaaaattca tgcagtaatg ggaaaggacg ctcattgctg
      481 ttcaccgcag ttctgaatta ttgaatttat aaaggtgaat gatgcacgaa aagaacatcg
      541 ccctgctttg tgatgaagcc gaccggcttt tgcaactgaa tatcaatctg ctccggcaaa
      601 tggttgatga gccggatgtg ttatctgaca gtaagaacga aaacgggctg ctttttgata
      661 aacggaaagc gctgaaaaga attgaggagc tggagggcga acaaatcaaa accgcccgca
      721 gggagatggt gctggctgtg gtcggcacga tgaaggcggg caaatcaacc accatcaacg
      781 ccattgtggg gcaggaaatt ctgcccaacc gtaaccgccc catgacctcg gtaccgacat
      841 tgatccgcca cgttcccgga aaaacggaac cggtcctcca tctggaacat attcagcccg
      901 tccgcaattt attaatcaca ctgcaggaaa aactcgccac cccggcaggg cagcaagtcg
      961 cacagtccct gcagcaaacc ggggataccc gcgaactact ggatattctg gcggatgatg
     1021 tctggcttaa aaatgaatac cacggggaag acgaaatctt taccggactg gcatcgttaa
     1081 atgacctggt tcgtcttgct gcggcaatgg ggactgaatt tccttttgac gaatacgcag
     1141 aagtgcagaa actaccggtg atcgacgtgg cgttcagcca cctggtggga atggatgcat
     1201 gtcagggaac actcacactg ctggataccc ccggccctaa tgaagccgga caaccacaga
     1261 tggaaatgat gatgcgggat caactacaga aagcctctgc ggtgctggcg gtgatggatt
     1321 acacccagat gaactcgaaa gccgatgagg aggttcgtaa agagcttaat gccattgctg
     1381 acgtctcagc cggtcgtctg tttgtgctgg tcaataaatt cgatgaaaaa gatcgcaatg
     1441 gtgatggtgc ggatgccgta cgccagaagg tgccagcaat gctgaacagc gatgtgctgc
     1501 ccgcctcccg cgtttatccc ggctcatcac gtcaggcgta cctggctaac cgtgcgcttc
     1561 atgagttacg aaaaaacggt gcgcttccgg ttgatgaagc ctgggtcgat gattttatca
     1621 gggaagcttt cggacctatg gtggaagagg acgactggaa agacagcact aaggtaaata
     1681 aaaaggcaga aaaactctgg aatatatctc tgatcgatca actgataacg gaagtcatac
     1741 agagctccca ttccagagcg gcggcactgg cggttgactc tgccgccgca aaactgatgc
     1801 agaatgcaga aaatgtcagc gaatatctgt cattacgtca tcaggggctg cagcaaagca
     1861 ttcagtcact acaggcgcat attaccagcc tgctggcgga tatccaggag attgaggaat
     1921 gtcagaatca ggtgaccggt gatgtcagaa tggccatgga ggacatcaat accaaaaccg
     1981 gggaattact gacgaaggtc tgtgcctcac tggaggagga gctgaacgac tatttcagaa
     2041 gcggtaaacg caaagaacag caaatgctgg aggaagaaaa ttcagcgcag ccccgggaga
     2101 gaaacgcatt cgctttcttt cacgatattt tcggaaccgg aaatcagcat gacaggatgc
     2161 gggactttga tccggacagc cccgaaataa aattcagtga tcgcagggca gcccttgaac
     2221 tgatgacgca aatcgaatcg accgtgacca gcctgcaccg tgaggctgaa gcacagttcc
     2281 ggcctgagct ggagaaaatc gtcagcggga ttgaaacagg ttttcgtggc acggccctgt
     2341 acgccacaga aaacattgcc ggtcgcatca atgcccgcct ggaggatgag ggctttaccg
     2401 taaaaatcag ttttccggca gtcagccagt tacagacccg gctcgcggta aaaataaatc
     2461 tgagtgcgct tatggaggaa agaacggaga ccgtcacccg tcgccgtcgg cagagtggcg
     2521 tatggggaac cgtttgtcga tggtttggca ccagtgacct gggctgggaa aactatgacg
     2581 aggatgtgag tcgcagcgtg atcaatatca acaaggccag agaggaagtt atgtcactga
     2641 cccgggcata tttcggggag ctgcaggcat ccattgagca ggatattaac cagcccgtcc
     2701 gccaggaaat cgatgccttt ttctgcgcat tcagggagaa agttgaacaa ctgcgtaaca
     2761 cgctgattca gagctctgaa gatcataaac gcgatcagca ggcgcaggaa cggcttaccg
     2821 ggcgacttca ggcattaaat gaaagggttc ctgagctcat tactgacagt aaggcgctga
     2881 gggaagagct ggagacaatg ctgtgacttc accatttatt caacaaattg ctgataaccg
     2941 ggtatgtcag gtactcacct gtcttcctga aaaatttgtg gttgatttcg caaacggaat
     3001 cgatgttgca caggagcata tccgcacggc cggagagcgc acgttcttcc ggcgtttaaa
     3061 agaggggctg accggcgaag gcgctgcacg gcagaatgcc atcaatgcgt cgcttgcaca
     3121 gggcgttgag gcgtccctgc gctggctgac ggagatgaca acgtcacttg ccaccacaaa
     3181 ctacgcgatt acccgggtaa acgacagggt cagttcactg gtcagtgata cagccaggct
     3241 ggcgcattat tccgcagata cgcgggagca gttactcacc ctggccgatc aggttcacca
     3301 taaactgaat catcttgaag aaaaactcca ccgtgttgac caggttcagc gggcgcagtt
     3361 acatcttgag cagatattct catggtggag cgccgggcga tatgcgtcat tttcccctgc
     3421 cggacgttgt tatgtggcac ttgaagagct tcgctggggc gcgtttggtg atgtgatacg
     3481 tcagggcgaa acaggccagg ttaaccagct actggatatt ctcagacata aagcattaac
     3541 gcagatggca caggagagtg gcggtagtgc aaccgtgcgt ctgaacactc tggactggct
     3601 tggtggtcag ggccgggagc aggcagataa cgagtggcat gacgcgatta actggctggg
     3661 agactggtgc agtgaagagc agcatccggt gatctggtca accacgcagg ctgcagaaca
     3721 cttaccggtt cgcatgcctc gcctctgctc tgcagaacgc ctctctgaaa gtatggtcga
     3781 tgaaatattt cagaaggggg ctgcatgagt acagaaatga aaacggggct ggtgctgtcc
     3841 ggtggcgggg cggtgggcgc ttatcaggcg ggagtggtta aggcactggc agagtgtggt
     3901 acacagatca gcatggtttc agggaccagc attggcgcat tcaatggtgc cattatcgcg
     3961 gcctctcccg atctgtcaga agctgccgta cgcctggagg cgctctggga gcatctgggg
     4021 aataatcagg tgctgtcggt aaacagattg gtttactttt cattgctgaa aaaattgttc
     4081 caggcaatga acctctgcca gatccccgga cgtgcaggag cactgcttac gacgcttctt
     4141 cgccatatat cgacaatcaa cgggtttgac aatctgatgg ctcagccgtt gttgtcagat
     4201 gagcccctga cagcgctgat ggatcattat cttgatactg atgctctggc agacgggcta
     4261 ccgctgtatg tgtcgctgta ccccacagaa ggggcatgca ggatattatt gactgcattc
     4321 gtgctgaact gggtgtcgga accacgaaaa acgccgtttt tcagcatatc cagagcctgc
     4381 cccgcggaca gcagaaagag gctctgcttg cgtcagccgc gctgcccctg ctgttccgtc
     4441 cccgtgaggt tcaggggaca atgttcggtg atggtggtat gggaggatgg cgaaatatgc
     4501 agggaaatac ccctgtgacg cctctggtcg atgccggatg caatatggtg attgtgacgc
     4561 atctgagtga cggttcttta tgggatcgcc aggcttttcc ggacaccaca atccttgaga
     4621 tccgtccccg gaaaaggctg aaatatgcag gtgatggtgg caacagcggc ggtctgctca
     4681 gttttacatc ggcacatacc gacgcctggc gtcagcaggg ctatgaagac acgatgctgg
     4741 cgatggagca tatccggaaa ccgctggcag cacgtcaggc actgacccgg tcagaggcgg
     4801 tattgcagaa aagcctggat ataacggaag aggcagattt ggcactg
//
LOCUS       DAZGLJ010000287         4801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000287 DAZGLJ010000000
VERSION     DAZGLJ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.287"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(25..2661)
                     /gene="fimD"
                     /locus_tag="ACHK49_004560"
     CDS             complement(25..2661)
                     /gene="fimD"
                     /locus_tag="ACHK49_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO9265060.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKTMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(2728..3453)
                     /gene="fimC"
                     /locus_tag="ACHK49_004561"
     CDS             complement(2728..3453)
                     /gene="fimC"
                     /locus_tag="ACHK49_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO9265061.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(3490..4029)
                     /locus_tag="ACHK49_004562"
     CDS             complement(3490..4029)
                     /locus_tag="ACHK49_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9265062.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(4094..4642)
                     /gene="fimA"
                     /locus_tag="ACHK49_004563"
     CDS             complement(4094..4642)
                     /gene="fimA"
                     /locus_tag="ACHK49_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO9265063.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         1197 a         1250 c         1066 g         1288 t
ORIGIN      
        1 aggtttgttt ctcatcacgc ccccttaacg acattcagct gatagctggg ttaataactg
       61 ctgctgactc tctggtggca gttgataatt ggcgacacag tgagcatttt cctcttctcc
      121 ccatttcacc tgaacttttc ccgctaaagg cattccgctg aggtaaacct gaccattatc
      181 cgcaacaatg ccgctactct ggctactctc tgatgtcacc atcgccccaa acggcagcgg
      241 cttattattg tgggttagcg tcatgagcag ttttatccca acgcgcgctt taaactctgc
      301 tcgcacgatc gccccacgag tgggaacaac gttagcgacc gcgttatcta aatcgacgtt
      361 atcagccagg gtattggtat ccagcgccac tctgttttcc ctatattcag tggcataagg
      421 cagcacggca taaccgcgcc agtcggtacg cacccccgtc tggttttcga cttttgcatc
      481 ttttgcgcca ggcgctttaa caagcaccac cgtatcgttt aacggctgcc ccagcgttac
      541 gccattggca tgagccaata ccccaccgct gactccgtaa tagagctgct taatatcatc
      601 gctatggctg taaccgatat tggcattgcc gtaaccaccg cgataattca gcgtggcgta
      661 gcctgtgctt ccgctattac catcgcctcc cccggcatag ccggtttgca cgctatagct
      721 gaggttgttg tcttccagca acgtaccgta tacaccagcc agattggtca tccgaccgtt
      781 gagatcgtgt gacatgctgt agctggcact ggcatgtcgc cactgagatt tactgtcaga
      841 acgcagccag tggctgaaag gaatattgac gttaagcgct aacatctgat cacgtccttt
      901 ttgccaggcg tttttcgtca ggctatagct gagcgtccag ttgatatctt cgaacgcagt
      961 atttaatcca gcctggaatt gctcatcgac attactcgtt ccccaataag tttgatggct
     1021 accactcaaa tacagtgttg atgtgcgccc gagttgctga gtaacggtga gctgtaattt
     1081 cccgcgtttg ttataagcga ggttgtaata gtcggtgaat ttcggcttaa cctgaataac
     1141 tccatcctgt gtttcgatgt tgtagccatt cattcgactg tatgttgtat cagcgaaatt
     1201 aaaatatccg ctggtcgaat aacggtaacc cactaactga atattcgtgc ctgattcatt
     1261 gagcgatttg ttatagagaa aacgcaccga ttgtccgtca tgctgactgt catcgggaag
     1321 tgtggaatta gcctgcgtca tatccacaga cagagcgccc agtgccccca tggttttccc
     1381 gataccgaaa ttaaaagcac gataacgatc cgccagttgc gttccaccat atattgtcca
     1441 gccagccgga aggccgtgga gtaatgtact ctggaaaaag cggggttttt cctgctgcgc
     1501 atttccacta cggtattctc ctgccgtaat ggaataacga gtatgccctt cacgttgcaa
     1561 aagcgggact gacgaatagg gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat
     1621 cgttacctgc aagtcaccac tattacctgc ggcatagata tcgttgatgg taaaaggccc
     1681 cggtggcacc gtactattat aaatgtcata cccattttgt ttaatagtga cctgtgcagt
     1741 accacgagca ataccgtgga tcaccggggc aaatcctctt tggctatcgg gtaacatatt
     1801 gtcatctgag gccaattgtg cgccgcgaaa gttaatacca tcgaaaatat cgccctgagt
     1861 ataaccatca cccagcgtca gccgggaacg taacggtatt atgtctcgct caagccaggt
     1921 attgatatgc tgccatttat ttttgctacc tgatgatctg tcgctactgt tataactcca
     1981 ggtggtattg tcgcgtaaac gccacgcacc aatatttaac ccactctgta ggtttaaata
     2041 tgcataatgg ctgttacccc caatccgatt ctgtacacta tttccgctga aattataatt
     2101 gagcaatccg gcattaatac cgggatccca taactcagga ggaatataac cacgcgcgcg
     2161 attactcata aatgcctgag ggatcgtcag gttcagtcgc tgctgaccaa catccagatg
     2221 cgcagtagcg tcctggacca ttgtggttaa tggcacacag gcatcatccg ccagcagatt
     2281 cataccggcg acagaagccg tattcagccc catactggcg agttgcgcgc gtgtcaggca
     2341 gggaacaatc ccttgttcac tgtcgcccgt attaaatgtg acatcacgcg ttgccatata
     2401 accattattc aaatagatat cgacgcgata cgtccctggc ggtaattctt gcccattttc
     2461 aaaacgcgat aaatcggcca cagcctgggg atcatccgct aaaaaacgcg ggttaaaata
     2521 gagttcggca gatgacaaag gtgcctgtgc ggcaaaagca caggcaacaa agagccggac
     2581 aaaaaaacca gccaaacgat gcttacgaat atgcaagcat tgtgtgtttc gctggtaaag
     2641 tcttaaattc agatatgaca ttattcctcc ggtttaaagt cacccggcag tcaattcttt
     2701 ttattcaggc gaaaaattcc ccctgcgtta ttccattacg cccgtcattt tgggggtaag
     2761 tgcaccataa tcatttattg ttcggtaagt aatattgctt cccgcatcag aaggcaattt
     2821 aaccgtgctt tcgcccattg gaggcaccaa ggcattttca agaacccggg ttccggcatt
     2881 caactctgtt accgtcaggt aatagggtgt cgggttaatc agcgtcagag aattcgcgct
     2941 acgacgaaat cttaattttt ctgcggcctg atcgggtggc aacgctaatt tagccgggcg
     3001 atagtacagt ttaatgcggc tgataattgc gagctgtagc gtattctcag tcaattttga
     3061 tttatccatt gacggaatcg ctttaacatt catccagaat aaactttccc gatcctgtgg
     3121 caattggtta tttgttgcat caagaatacg caaggtattc tctttttttc ccttcatcgc
     3181 aaacagagga ggcgtcacga taaaacgacc atcctttaca ccatcggcat tttccaccca
     3241 tgattgaatt aaataggtac tattttcatc attatttgtc acggcaagtt gcacttgttt
     3301 ttgccctgcc ggataaatta cgcgagtcgc acctaaggcc actcccgctt cagcgcgtcc
     3361 ggcaaccatc attgccataa acatcaggat acctgccagc aagcagaatg ttatttcctg
     3421 cgatttcctt acattgacgt ttttattact cactgtcctg ttcctgttat cacattatct
     3481 gctgaacaat tactgatagg ttaaagagaa ccaggcctgg gcattggcga tgccgccagt
     3541 aacccgacgc ccggtagcac gatatttggc gatgaaatgt agcgaagtag agcctgagta
     3601 aagccgtttc cagtttgctg gaggacgatt aatcggtacg aggtttcctt catcatcaaa
     3661 caacgctacg ccaatattgg tggctatccc tggcccctct cccacggaaa gcacatccgg
     3721 atttttacca tccgcgacac cgtgaaacgc cacccccaca cgttcactca ccaccgtgct
     3781 acattcccgt aaatgaataa caaaaggcac cggtgcgcta tcttcccctg ccgcatgaaa
     3841 ccggttactg ctgatttgcc ccatattgac cgtcatttgt ttatcaccgg cttcaatccg
     3901 gcaagtttcc gcaataatga cgccctgaaa ttgcatattt ccgccgggca gcgtggtatt
     3961 ccatttattt ccggccagag caaacattgg cagcaacgac gctaacagaa ataatctctt
     4021 ccttttcatc gttattttta tcgcacaagg gtgggcatcc ctgcccgtaa tgacgtcctt
     4081 gaacctgggt aggttattga tactgaacct tgaaggttgc atccgcatta gctgcacccg
     4141 gggttgcctc gccgattgca taataacgcg cctggaacgg aatggtgttg gtaccgttat
     4201 tcagggttgt ttgtgcacta aatgtcgcac cgtccagcgt cagagcattg cctgttctgt
     4261 ccaggatctg cacgccaacg tttgttgcac tacctgcagc agaactctgc agagccagta
     4321 catcagtacg cgtagcatca attgccgtac ctaagaaggc aacagcggct tttgtggcaa
     4381 cagtggtatc gcaatcattc aactgaatgt taaaaccaac ggcagagctg gttgctccag
     4441 cctgcttcag gctagcggta cgaacctgtc ccaactgaac ggtttgatca acagagcctg
     4501 catcaactgc gcaagcggcg ttaacaactt cccctttaaa gtgaacggtc ccaccattta
     4561 ccgtcgtagt atcggccaga gccgctgcgg aactgaggga cagagccgac agaacaacga
     4621 ttgccagagt tttaattttc atgctgcttt cctttaaaaa aactatttct aaatcgacat
     4681 gggcagtcgt tctgtacact ttgttttgtc aacgagtttg actgccaaca ctgcacagtt
     4741 ttcccccaaa agatgaaaca tttggggcca ttttgactca tagaggaaag catcgcgcac
     4801 a
//
LOCUS       DAZGLJ010000288         4794 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000288 DAZGLJ010000000
VERSION     DAZGLJ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.288"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..652
                     /gene="fimC"
                     /locus_tag="ACHK49_004564"
     CDS             <1..652
                     /gene="fimC"
                     /locus_tag="ACHK49_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO9265064.1"
                     /translation="LIISASAHAAGGIALGATRIIYPADAKQTAVWIRNSHTNERFLV
                     NSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAADRESLFWMNVKTIPS
                     VDKNALNGRNVLQLAILSRMKLFLRLIQLQELPAEAPDTLKFSRSGNYINVHNPSPFY
                     VTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDYGSVTPVREVNLN"
     gene            683..3292
                     /gene="sfmD"
                     /locus_tag="ACHK49_004565"
     CDS             683..3292
                     /gene="sfmD"
                     /locus_tag="ACHK49_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO9265065.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERISLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGIRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGTVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGRKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            3334..4311
                     /gene="fimH"
                     /locus_tag="ACHK49_004566"
     CDS             3334..4311
                     /gene="fimH"
                     /locus_tag="ACHK49_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO9265066.1"
                     /translation="MACLCLANISWATVCANSTGVAEDEHYDLSNIFNSTNNQPGQIV
                     VLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLHDYLLGAMSLVD
                     SVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMVEIPRQVMFTV
                     YVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASLFSAAGPGNRP
                     AGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIVADQNDTPITP
                     NDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDYQ"
     gene            4322..>4794
                     /gene="sfmF"
                     /locus_tag="ACHK49_004567"
     CDS             4322..>4794
                     /gene="sfmF"
                     /locus_tag="ACHK49_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO9265067.1"
                     /translation="MRRVLFSCFCGLLWSSIGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPG"
BASE COUNT         1234 a         1064 c         1183 g         1313 t
ORIGIN      
        1 tttaatcatt tcggccagcg cccatgctgc cggagggatc gcattaggtg ccacgcgtat
       61 tatttatccc gctgatgcta aacagactgc ggtatggatt agaaatagcc ataccaatga
      121 gcgctttctg gtcaattcgt ggattgaaaa cagcagcggt gtaaaagaaa agtcattcat
      181 cattacaccg ccactgtttg ttagtgaacc caaaagcgaa aataccttgc gtattattta
      241 caccggtcca ccgctggcag cagatcgtga gtctctgttc tggatgaatg ttaagacgat
      301 cccttcggta gataaaaatg cattgaacgg caggaatgtt ttgcaactgg cgattttatc
      361 gcgcatgaaa ttatttctcc gtctaattca attacaagaa ttacccgcag aagcaccgga
      421 cacactcaag ttttcgcgat ccggtaacta tatcaatgtt cataatccct caccttttta
      481 tgtcactctg gttaacttac aagtgggcag ccaaaagttg gggaatgcta tggctgcacc
      541 cagagttaat tcacaaattc ccttaccctc aggagtgcag ggaaagctga aatttcagac
      601 cgttaatgat tatggttcag taaccccggt cagagaagtg aacttaaact aaccgaatca
      661 tctgacaata tcagagctaa ttatgaaaat acccactact acggatattc cgcagaggta
      721 tacctggtgt ctggccggaa tttgttattc atctcttgcc attttaccct cctttttaag
      781 ctatgcggaa agttatttca acccggcatt tttattagag aatggcacat ccgttgctga
      841 tttatcgcgc tttgagagag gtaatcatca acctgcgggc gtgtatcggg tagatctctg
      901 gcgtaatgat gagttcattg gttcacagga tatcgtattt gaatcgacaa cagaaaatac
      961 aggtgataaa tcaggtgggt taatgccctg ttttaaccag gtactccttg aacgaattag
     1021 ccttaatagc agtgcatttc ccgagttagc ccagcagcaa aacaataaat gcatcaattt
     1081 actgaaagct gtacctgatg ccacaattaa ctttgatttt gcagcgatgc gcctgaacat
     1141 cactattcct cagatagcgt tgttgagtag cgctcacggt tacattccgc ctgaagagtg
     1201 ggatgaaggt attcctgctt tactcctgaa ttataatttc accggtaaca gaggtaatgg
     1261 taacgatagc tattttttta gtgagctcag cgggattaat attggcccgt ggcgtttacg
     1321 caacaatggt tcctggaact attttcgcgg aaatggatat cattcagaac agtggaataa
     1381 tattggcacc tgggtacagc gcgccattat tccgctgaaa agtgaactgg taatgggaga
     1441 cggcaataca ggaagtgata ttttcgatgg tgttggattt cgtggtatac ggctttactc
     1501 ttccgataat atgtatcctg atagccagca agggtttgcc ccaacagtac gtgggattgc
     1561 ccgtacggcg gcccagctaa cgattcggca aaatggtttt attatctatc aaagctatgt
     1621 ttcccccggc gcttttgaaa ttacagattt gcacccgaca tcttcaaatg gcgatctgga
     1681 cgtcaccatc gacgagcgcg atggtaatca gcagaattac acaattccgt attcaacagt
     1741 gccaatttta caacgcgaag ggcgtttcaa atttgacctg acggcgggcg attttcgtag
     1801 cggtaatagt cagcaatcat cgcctttctt ttttcagggt acggcactcg gcggtttacc
     1861 acaggaattt actgcctacg gcgggacgca attatctgcc aattacaccg cctttttatt
     1921 agggctgggg cgcaacctcg ggaactgggg cacagtgtcg ctggatgtga cccatgcgcg
     1981 cagtcagtta gccgacgaca gtcgtcatga gggggattcc attcgcttcc tctatgcgaa
     2041 atcgatgaac accttcggca ccaattttca gttaatgggt taccgctatt cgacacaagg
     2101 tttttatacc cttgatgatg ttgcgtatcg tcgaatggag gggtacgaat atgattacga
     2161 ttacgacggt gagcatcgcg atgaaccgat aatcgtgaat taccacaatt tacgctttag
     2221 ccgtaaagac cgtttgcagt taaatatttc acaatcactt aatgactttg gctcgcttta
     2281 tatctctggt acccatcaaa aatactggaa tacttcggat tcagatacct ggtatcaggt
     2341 ggggtatacc agcagctggg ttggcatcag ttattcactc tcattttcgt ggaatgaatc
     2401 tgtagggatc cccgataacg aacgtattgt cggacttaat gtttcagtgc ctttcaatgt
     2461 tctgaccaaa cgtcgctaca cccgggaaaa tgcgctcgac cgcgcttatg cctcctttaa
     2521 cgccaaccgt aacagcaacg ggcaaaatag ctggctggca ggtgtaggtg ggaccttact
     2581 ggaaggccac aacctgagtt atcacgtaag ccagggtgat acctcgaata atgggtatac
     2641 gggcagcgcc acggcaaact ggcaggccgc ttacggtacg ctgggggtcg ggtataacta
     2701 cgaccgcgat caacatgacg ttaactggca gctgtctggc ggtgtggtcg ggcatgaaaa
     2761 tggcataacg ctgagccagc ctttagggga taccaatgtt ttgattaaag cgcctggcgc
     2821 aggcggtgta cgcattgaaa atcaaactgg cattttaacc gactggcgcg gctatgcggt
     2881 gatgccgtat gccacggttt atcggtataa ccgtatcgcg cttgatacca atacgatggg
     2941 gaattccatc gatgttgaaa aaaatattag cagcgttgtg ccgacgcaag gcgcgttggt
     3001 tcgtgccaat tttgataccc gcataggcgt gcgggcgctc attaccgtta cccagggcag
     3061 aaaaccggtg ccgtttggat cactggtacg ggaaaacagt accggaataa ccagtatggt
     3121 gggtgatgac gggcaagttt atttaagtgg tgcgccattg tctggtgaat tactggttca
     3181 gtggggagac ggcgcgaact cacgctgcat tgcgcactat gtattgccga agcaaagctt
     3241 acagcaagcc gtcactgtta tttcggcagt ttgcacacat cctggctcat aaaggaaatt
     3301 atcaataaga taatctgcag attattattg gcgatggcat gtttgtgtct ggcaaacata
     3361 tcctgggcta ctgtttgtgc aaatagtact ggcgtagcag aagatgaaca ctatgatctc
     3421 tcaaatatct ttaatagcac caataaccag ccagggcaga ttgttgtttt accggaaaaa
     3481 tccggctggg taggtgtctc agcaatttgt ccacccggta cgctggtgaa ttatacatac
     3541 cgtagttatg tcaccaactt tattgttcag gaaactatcg ataattataa atatatgcaa
     3601 ttacatgatt atctattagg tgcgatgagt ctggttgata gtgtgatgga tattcagttc
     3661 cccccgcaaa attatattcg gatgggaaca gatcctaacg tttcgcaaaa ccttccattc
     3721 ggggtgatgg attctcgttt aatatttcgt ttaaaggtta ttcgtccctt tattaacatg
     3781 gtggagatcc ccagacaggt gatgtttacc gtgtatgtga catcaacgcc ttacgatccg
     3841 ttggttacac ctgtttatac cattagtttt ggtggccggg ttgaagtgcc gcaaaactgc
     3901 gaattaaatg ccgggcagat tgttgaattt gattttggtg atatcggcgc atcgttattt
     3961 agtgcggcag ggccggggaa tcgaccagct ggcgtcatgc cgcaaaccaa gagcattgcg
     4021 gtcaaatgta cgaatgttgc tgcgcaggct tatttaacaa tgcgtctgga agccagtgcc
     4081 gtttctggtc aggcgatggt gtcggacaat caggatttag gttttattgt cgccgatcag
     4141 aacgatacgc cgattacgcc taacgatctc aatagcgtta ttcctttccg tctggatgca
     4201 gctgcggcag ccaatgtcac acttcgcgcc tggcctatca gtattaccgg tcaaaaaccg
     4261 accgaggggc cgtttagcgc gctggggtat ttacgcgtcg attatcaatg aggtacggag
     4321 aatgagaaga gtactcttta gctgtttctg cgggttactg tggagttcca ttggatgggc
     4381 agctgaccct ttaggaacga ttaatatcaa tttgcacggt aacgttgttg atttctcctg
     4441 taccgtaaac acagcggata ttgataagac ggtagattta ggcagatggc ctacgacaca
     4501 attactgaac gctggcgata ccacggcact cgtccctttt agcctgcggc tggagggatg
     4561 tcctccgggt tcagttgcga ttttatttac gggaacgccg gcatccgata ccaacctgct
     4621 ggctctggat gatcccgcaa tggcacaaac cgtcgccatc gaattacgta atagcgatcg
     4681 ctcccggctc gcactggggg aggcgagccc gactgaggaa gtagatgcaa atggcaatgt
     4741 cacactaaac ttttttgcca attatcgagc gttagccagc ggtgttcggc ctgg
//
LOCUS       DAZGLJ010000289         4649 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000289 DAZGLJ010000000
VERSION     DAZGLJ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.289"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            40..815
                     /locus_tag="ACHK49_004568"
                     /pseudo
     CDS             40..815
                     /locus_tag="ACHK49_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(974..1264)
                     /locus_tag="ACHK49_004569"
     CDS             complement(974..1264)
                     /locus_tag="ACHK49_004569"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9265068.1"
                     /translation="MSRRWQANWPNLTTFFAYLTDIRKVIYTTNAIESLNSVIRHAIK
                     KRKVFPTDDSVKKVVWLAIQSASQKWTVPLKDWRMAMSRFIIEFGDRLNGHF"
     gene            complement(1314..1412)
                     /gene="astA"
                     /locus_tag="ACHK49_004570"
     CDS             complement(1314..1412)
                     /gene="astA"
                     /locus_tag="ACHK49_004570"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-stable enterotoxin EAST1"
                     /protein_id="HFO9265069.1"
                     /translation="MRRPTSSYASCIWCATVCASCHGRTTKPSLAT"
     gene            complement(1366..1527)
                     /locus_tag="ACHK49_004571"
                     /pseudo
     CDS             complement(1366..1527)
                     /locus_tag="ACHK49_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558334.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1581..2459)
                     /locus_tag="ACHK49_004572"
     CDS             complement(1581..2459)
                     /locus_tag="ACHK49_004572"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO9265070.1"
                     /translation="MKLKMQDLRLFNIVFESDPGWILDFSNRTLSAFFDEELNIDIDD
                     ERYQKEGASKAKRVRCLLKQVDRETALRVLGALWQYKTESMPEQAEQSRNDYLALISR
                     LENADTDEAKGVKPVQAWHGVDWHSLIAEMNEMKSLPPHPRGFRFEAWLAELFSIFKL
                     APRSSFRNTGEQIDGSFRLNDEFYLMEAKWHQKRTSAADLHVFEGKLSTKATWTRGVF
                     ISWMGFTPEGLTAFGKGKRVICVSGYDLYHSLNHRIPLPDLLDAKTRHAAETGEPYAE
                     FDRLYALKDKQFGTLK"
     gene            complement(2787..3518)
                     /locus_tag="ACHK49_004573"
     CDS             complement(2787..3518)
                     /locus_tag="ACHK49_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO9265071.1"
                     /translation="MNQPIHNAYWLSRFESILNSALAQHRAVSLIRVDLRFPEYMPAT
                     IMDTDLDSAVISRFFASLKAKIQAYQRHKRRANKRVRATTLRYFWCREFGKEKGRKHY
                     HVILLLNKDTWCSPGDFTVPSSLATLIQLAWCSALHLEPWQGNGLVHFSRRTPFRKPT
                     SSDARPSSDDTPVPGGCSETRKVSDKKSGEAAVLWIKRGDVEAMQKARNRASYLVKNE
                     TKLHDGSGQRNYGCSRGPGRLLDGR"
BASE COUNT         1379 a         1082 c         1036 g         1152 t
ORIGIN      
        1 atcttaatat tagtttttag actggtcatt ggaaaacaga tgattgatgc ctcaggaccg
       61 gagaaactca gacggcgtac tgcacaggaa aagatcgcta atgttcagaa gagctttgaa
      121 ccaggaatga tggtctccct cgttgcccgg caacatggtg tagcagccag ttacttctct
      181 ggcgcaagca ataccaggag ggaagtctta ctgcggtgac tgctggggaa cagatcgttc
      241 ctgcctctga acttgctgct gccataaggc agattaaaga acgccagcgc ctgctcggaa
      301 caaaacgatg gaaaatgaac tcattaaaga agccgttgaa tatggacgtg caaaaagtgg
      361 atagcgcacg cgcccttgtt gccggggtga tgcactgggc ggtcactacc ggcggcttca
      421 acagtgaaac agcacaggac gtcatgccag gagcggtgga acgccgcttc ggcaacgagc
      481 ttccggcgtc tccagtggag tggctgacag ataatggttc atgctaccgg gctaatgaaa
      541 cacgcctgtc cgctcggatg ttgggacttg aatcgaagaa cacggcggtg cggagtccgg
      601 agagtaacaa aatagcagag agcttcgtga aaacgataaa gcgtgactac atcgtgttca
      661 tgcccaagcc agatgttaat gacggcattg cataaccatg cagtagcgat cgaacattac
      721 aatgcagcat agctctaggt tatcgctcgc cgcaggtata tatgtggcag cggatcagta
      781 atgggttaaa tgataacagg agtctggaaa tatagggaga aattccctga tggatcccca
      841 aaaaatttag taaaccaata tcatattggt ataccgttgt tgtagaactt tcagccctgc
      901 gtcaagtgac aagatgttta gtatgagtga agaatgtcgg aacctgttta ggattctgtg
      961 taaatgcctt ttctcagaag tgaccgttca ggcggtcacc gaactcgata ataaagcggc
     1021 tcattgccat acgccagtcc ttcaatggca ctgtccattt ctgggacgca gactggattg
     1081 ccagccacac cacttttttc accgagtcgt ctgtcgggaa cactttacgc tttttgatcg
     1141 catggcggat cacactgttt agtgactcga tggcatttgt cgtatagatc actttgcgga
     1201 tgtccgtcag ataagcgaag aacgtagtaa gattcggcca gttagcctgc cagcgtcggc
     1261 ttatctagtg cttgctagcc tgcctcttcc gtgggtgcct gattaaatct ctttcaggtc
     1321 gcgagtgacg gctttgtagt ccttccatga cacgaagcgc agactgttgc gcaccatatg
     1381 cacgatgcat aactggatgt gggtctccga atatactgtg ttgatggcat ctgggaagcc
     1441 tttcaggcca tctgcacaga tgataaggat attgttcagg ccgctgtttt tcagttcagc
     1501 cagcacattg agccagaact tcgaccctgt acacgatttt gtgtgaaaca acggggagca
     1561 ggtcatcgct tcccaacccg ttatttcagc gtgccgaact gtttgtcttt cagtgcatat
     1621 agtcggtcga actcagcata gggctctccc gtttcagccg cgtgccgggt tttagcatcc
     1681 agaaggtcgg gtaaagggat cctatgattc agtgagtggt aaagatcata accactgaca
     1741 cagataaccc gcttcccttt accaaaagcc gtcaggcctt caggcgtaaa ccccatccaa
     1801 ctgataaaaa caccgcgtgt ccaggttgcc ttggtactga gttttccttc aaatacatgg
     1861 aggtcggcag ccgacgttct tttctgatgc cactttgcct ccatcaggta aaattcatca
     1921 ttcagccgga aacttccgtc aatctgttct cctgtatttc ggaaagacga acgaggagca
     1981 agcttaaaga tactgaacag ttcagcaagc caggcttcaa accgaaaacc acgtggatgt
     2041 ggcggcagag atttcatctc gttcatttca gcgataagtg aatgccagtc aacaccatgc
     2101 catgcctgga caggcttaac gcctttggct tcatcggtgt cagcgttttc taagcgggaa
     2161 atcagtgcaa gatagtcatt acggctttgt tcagcctgtt caggcatgga ttccgtttta
     2221 tactgccaga gcgcaccaag aacccgcaga gcggtctcac gatcaacctg tttcagcaag
     2281 catctaactc gtttggcttt tgacgccccc tctttctgat aacgttcatc atcaatatca
     2341 atgttcaatt cttcatcgaa aaaagcagac aaggtgcggt tagagaaatc cagtatccag
     2401 ccaggatcgc tctcgaacac gatattaaac agcctcaaat cctgcatttt cagtttcacg
     2461 cctacctcct tttttcacag tataacggga tgaaagtggg ggaggaaatg agtcagaacc
     2521 tgtacaccag cattagggga ttccgcgtct ggtacaaagc agactatgag aagagagacg
     2581 ctataacatt tgagtcctac caatcgatct gttcatttct atgaatggca gtaaatgcaa
     2641 ataaccagaa caaccaaacg tttttcgact tatgtgtcac gaacggaagt agatggcaat
     2701 ccattgcatt acttcaggac gacttttcag aaagcgagct aagggcgttg gcataaactc
     2761 aggcatcgga tgctgtttta cagggctcac ctgccatcca gtaaacgccc cggcccacga
     2821 ctgcaaccat aattacgttg acctgaacca tcatgcaact tcgtttcatt tttcacgaga
     2881 taactggctc tgttccgggc tttctgcatc gcttccacat caccacgctt gatccagaga
     2941 acagcggctt caccagactt tttgtctgaa accttcctgg tttcagaaca tccaccaggc
     3001 acaggcgtat catcggaaga agggcgagca tcggatgatg tcggtttacg gaaaggcgtc
     3061 cgcctggaaa aatgaaccag tccattaccc tgccagggct caagatgcag agcactgcac
     3121 catgccagtt ggatcagcgt cgccagcgaa gaaggaacgg tgaaatcccc tggcgagcac
     3181 caggtatctt tgttgagcag taatatcacg tgataatgtt tcctgccttt ttccttgcca
     3241 aactcccgac accagaaata acgcagagtg gttgcacgca cacgcttgtt ggcacgtcgt
     3301 ttatgtcgct ggtaagcctg aatttttgct ttcagggatg cgaaaaaacg agaaatcacc
     3361 gctgaatcta aatcggtatc cataatggtg gcaggcatat actcagggaa acgtaaatcc
     3421 acccggatta acgagacggc acggtgttgc gccagggcac tgttgagaat actttcaaaa
     3481 cgggataacc agtaagcatt gtgtattggt tgattcatga tgagagatct ccattgattt
     3541 ggattgactt cacatcatta atcaccgcgg taaaacttta cgaactccca ccattgagca
     3601 acacaaccta ctccggataa aaatggaatc ccctacaaac gaaaagatgg taaaaaaaca
     3661 caacaacagc aaatgaattt aaaaacggca tggacgacga gaaaagtcag aagggaaacg
     3721 gatcaacaga acgtgcaaac aaaacatcaa aaacgcaatc agaacgattt ctggcgctgt
     3781 gaatcaacaa caacaccaaa acctacagct taaccacaca actcgatcac agagccgaat
     3841 agcgcgctta aaacctgttt acagataacc ccaccacaag aacaaaaact accaatatca
     3901 gaatcgattt tcttatcctg aaaacgttac ttttcaacac accataaaaa cgattaactg
     3961 cgataacgta aacctcatct gaatcaaaat atcaaaaccg ttcatcacac tttcgcaatg
     4021 caagaagaaa cctcatgaca aaaagaatga ccttacacgg aaaagaaaac gtcaggaatc
     4081 gttaatgcct caatctgatt gttatcaaaa catccagaaa gtcgtttaac agaatcgtcg
     4141 taagcacatc gaaaaaaacg tcagattact tcaggaaagt tcgggttgat ttagcgtaaa
     4201 gatacttttt catgctaacg tcgaaaattc gaaatctcag gaaaagttaa acgtaacatc
     4261 agtaaaatcg aacagcaggt tgattgctta aacaggaaaa attgttttgt agcagcttta
     4321 aactgatttt agctaacgta aagatttact aaactggatg tgtatcagac aaagcaatgt
     4381 gtataacaat acttaatgaa aacacttgta gaagtgttta tgaaaatacg tgtatgtctg
     4441 gtaaaacact tagtagaaaa tacgtatata acacttattg aaatgcttag taaaaatacg
     4501 tgtatataat atcataccga cattagcaac attctcagac actcatgcag actgaatgtt
     4561 gattacccgt tttcacatca ggcatggttc tttactgacc aaaaaacaaa ctggtttact
     4621 cttgaattat tttctctacc tcagaaaac
//
LOCUS       DAZGLJ010000290         4628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000290 DAZGLJ010000000
VERSION     DAZGLJ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.290"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..596)
                     /locus_tag="ACHK49_004574"
     CDS             complement(<1..596)
                     /locus_tag="ACHK49_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO9265072.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDS"
     gene            complement(711..786)
                     /locus_tag="ACHK49_004575"
     tRNA            complement(711..786)
                     /locus_tag="ACHK49_004575"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(751..753),aa:Thr,seq:ggt)
     gene            complement(793..867)
                     /locus_tag="ACHK49_004576"
     tRNA            complement(793..867)
                     /locus_tag="ACHK49_004576"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(832..834),aa:Gly,seq:tcc)
     gene            complement(984..1068)
                     /locus_tag="ACHK49_004577"
     tRNA            complement(984..1068)
                     /locus_tag="ACHK49_004577"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1032..1034),aa:Tyr,seq:gta)
     gene            complement(1077..1152)
                     /locus_tag="ACHK49_004578"
     tRNA            complement(1077..1152)
                     /locus_tag="ACHK49_004578"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1117..1119),aa:Thr,seq:tgt)
     gene            1514..2464
                     /gene="coaA"
                     /locus_tag="ACHK49_004579"
     CDS             1514..2464
                     /gene="coaA"
                     /locus_tag="ACHK49_004579"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO9265073.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2493..3458)
                     /gene="birA"
                     /locus_tag="ACHK49_004580"
     CDS             complement(2493..3458)
                     /gene="birA"
                     /locus_tag="ACHK49_004580"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO9265074.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3455..4483)
                     /gene="murB"
                     /locus_tag="ACHK49_004581"
     CDS             complement(3455..4483)
                     /gene="murB"
                     /locus_tag="ACHK49_004581"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO9265075.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1179 a         1169 c         1050 g         1230 t
ORIGIN      
        1 taagaatcca ggaagccagc cagttccagg attttcgctt cccactctgc gtcgccttcc
       61 agcgctttca gagcagaacc acgaacgatc ggagtgtcgt cgcccgggaa gtcatactga
      121 gacagaagtt cacgaacttc catttcaacc agttccagca gctcttcgtc atcaaccatg
      181 tcgcatttgt tcaggaacac gatgatgtac ggaacgccta cctgacgacc cagcaggatg
      241 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaagat cgcgccgtcc
      301 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      361 tgtgcgtagt gacgggtcgg ggtgtcgtat tcaacgtgag aagtgttgat ggtgatacca
      421 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cgcgagcagc accgccgtag
      481 gttttagcca gtacggtagt gattgcagcg gtcagcgttg ttttaccatg gtcaacgtgg
      541 ccgatagtac cgacgttaac gtgcggtttt gtacgttcaa acttttcttt agacatcgat
      601 tgtccctcta agacacggat aaatcggtga tatcaccaca tcaaccaggc aacatgcccg
      661 acttgttgaa tgcaataaac agaaggaaaa aacagggagg agaaaagaag tggtgctgat
      721 aggcagattc gaactgccga cctcaccctt accaagggtg cgctctacca actgagctat
      781 atcagcacat cttggagcgg gcagcgggaa tcgaacccgc atcatcagct tggaaggctg
      841 aggtaatagc cattatacga tgcccgcatc ctggaactcg gctacctgat tttcattctg
      901 cactgaatat cgagagaagc tctctttatt cgagccggta agcgaactta tcgtctcggg
      961 ctacgccatc gcgtggccga aattggtggt gggggaagga ttcgaacctt cgaagtctgt
     1021 gacggcagat ttacagtctg ctccctttgg ccgctcggga accccaccgg acttgatggt
     1081 gccgactacc ggaatcgaac tggtgaccta ctgattacaa gtcagttgct ctacctactg
     1141 agctaagtcg gcatcaagta gcgcgcattc tatggagaca tgcgagttca tgcaactaaa
     1201 aaattgcata atttgtttta ttggtcacat tttatgcgac acgatgaaga aacagccgaa
     1261 cggagggcag attagcacac tttttcaaca tcattgtgct caacaatgcg ctcctgctaa
     1321 accataattc tttttatcag atggaatatc tgtcacattg cttttcaacg atagcttcct
     1381 ggcagagatt ttttcttatt attcctcccc atctggtgtt accctcctgc ccattaaccc
     1441 attcaacaga actgtgacgc gccatggcaa atatcgcttt gccgatagag ctatgaccgc
     1501 cagaaacatg cttatgagta taaaagagca aacgttaatg acgccttacc tacagtttga
     1561 ccgcaaccag tgggcagctc tgcgtgattc cgtacctatg acgttatcgg aagatgagat
     1621 cgcccgtctc aaaggtatta atgaagatct ctcgttagaa gaagttgccg agatctattt
     1681 acctttgtca cgtttgctga acttctatat aagctcgaat ctgcgccgtc aggcagttct
     1741 ggaacagttt cttggtacta acgggcaacg cattccttac attatcagta ttgctggcag
     1801 tgtcgcggtg gggaaaagca caaccgcccg tgtattgcag gcgctattaa gccgttggcc
     1861 ggaacatcgt cgtgttgaac tgatcactac agatggcttc cttcacccta atcaggttct
     1921 gaaagaacgt ggtctgatga agaagaaagg cttcccggaa tcgtatgata tgcatcgcct
     1981 ggtgaagttt gtttccgatc tcaaatccgg cgtgccaaac gttacagccc ctgtttactc
     2041 gcatcttatt tatgatgtga tcccggatgg agataaaacg gttgttcagc ctgatatttt
     2101 aattcttgaa gggttaaatg tcttacagag cgggatggat tatccacacg atccacatca
     2161 tgtatttgtt tctgattttg tcgatttttc gatatatgtt gatgcaccgg aagacttact
     2221 tcagacgtgg tatatcaacc gttttctgaa attccgcgaa ggggctttta ccgacccgga
     2281 ttcctatttt cataactacg cgaaattaac taaagaagaa gcgattaaga ctgccatgac
     2341 attgtggaaa gagatcaact ggctgaactt aaagcaaaat attctaccta ctcgtgagcg
     2401 cgccagttta atcctgacga aaagtgctaa tcatgcggta gaagaggtca gactacgcaa
     2461 ataatttgca ggggagcgaa tactcccctt tcttattttt ctgcactacg cagggatatt
     2521 tcaccgccca tccagggttt tattattcca tcctgctcaa gtaataaagc cccctgttta
     2581 tctattccgc gtgaaatgcc aaatatttct ttatcaccaa tgataagttt cactgggcga
     2641 ttaataaaat tatccagctt ttcccagcgc gacagataag gtgccaatcc ttcttgttcg
     2701 aagagttcca acgcagcacg taattcacgt attagcatgg ccgccaacgt attacgatcg
     2761 agattgatcc ccgcttcctg cagcgtgatc cacccctgat taacgacact ctcttcaaca
     2821 cggcgcattg ccatgttgat cccggctcca atgactattt gcgccgcatc gccagttttg
     2881 ccagtcagct ccacaagaat gcctgccagc ttgcgatcct gcagatagag gtcattaggc
     2941 catttgacac gaactttatc tgctcccagc ttgcgtaata cttccgccat cacgataccg
     3001 ataaccagac ttaaaccaat cgccgccgcc gggccttgtt ccagacgcca gaacatcgac
     3061 aaatataagt ttgcgccaaa aggcgaaaac catttccgcc cccggcgacc acggccagcc
     3121 tgctggtatt ctgcaacaca ggcatcgccc gatttaagct ctccgatacg atcaagaagg
     3181 tactgattcg tggagtcaat aactggcagc acggctacac taccgccatc cagctgaccc
     3241 aatatctgtt cagcattaag taactggatg ggctcaggca ggctgtatcc tttacccgga
     3301 acggtaaaga catcaacgcc ccagtcacgc agtgtctgaa tgtgtttatt aatagccgcc
     3361 cggctcattc ccagcgtttc acccaactgc tcgccagagt gaaattcacc gttcgctaac
     3421 agggcaatca atttcagtgg cacggtgtta tccttcatga aattgtctcc actgcgctca
     3481 cttcacctga tgcaccaata aagcggactt caggctcaag ccagacatta aatttttcac
     3541 ccactttttg tcttacatgg tgtgccagtt gcaccacatc ttcgcttttt gcattgtctt
     3601 cattaatgag aactaacgcc tgttgacggt gcaccgcagc cccacccatt tgcatccctt
     3661 ttagctggca ctgatcaata agccaacctg ctgccagttt tactgaacca cccgcctggg
     3721 ggtaatttgg tgctgttgga aattgcgcca gtaatgcttt agccgtttcg gcagatacaa
     3781 cagggttttt gaagaaacta ccggcattgc cattcacttt tggatcaggg agtttggtgg
     3841 tgcgcatatg acacaccgca ttaaatactt gctgtggcgt tactgttgta ggatccagac
     3901 gagttaagtc accatacgtt agtacaggtt gccactcttt tggcagacgc agacctacgg
     3961 ctacaatggc gaagcgatcc tggtattcat gtttaaaaat actgtcgcga tagccaaaac
     4021 ggcactcttt ggcagttaag cgcacttgct tgcctgtcgc cagttcaaca caatcaacat
     4081 aagcgcaaac tcgctgtaat tctacgccat aagcaccaat attctggata ggtgatgagc
     4141 cgacacaacc aggaattaat gccagatttt ccagaccagg cataccttcc tgcaaagtgt
     4201 attttaccag gcgatgccag ttttctccgg ctcctacatg taaataccac gcatcaggtt
     4261 catcatgaat ttcgatacct ttgatccggt tgatgatcac cgtgccgcga tagtcttcca
     4321 gaaaaagtac attacttcct tcacccagaa taagaacggg ttgtccttct gcggttgcat
     4381 actgccaggc attgagtaat tgttgttcgt cttcggcaca tacaatgtgc tgagcattat
     4441 gatcaatgcc aaatgtgttc cagggtttta aggagtggtt catagctgct ttcctgatgc
     4501 aaaaacgagg ctagtttacc gtatctgtgg ggggatggct tgtagatatg acgacaggaa
     4561 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta atttgatgcc
     4621 tggcagtt
//
LOCUS       DAZGLJ010000291         4609 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000291 DAZGLJ010000000
VERSION     DAZGLJ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.291"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(17..1519)
                     /locus_tag="ACHK49_004582"
     CDS             complement(17..1519)
                     /locus_tag="ACHK49_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO9265076.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDLMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMISELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASIEQQNNPPAKGKEIAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(1649..2668)
                     /gene="ahr"
                     /locus_tag="ACHK49_004583"
     CDS             complement(1649..2668)
                     /gene="ahr"
                     /locus_tag="ACHK49_004583"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO9265077.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDTC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGTDKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2864..2948
                     /locus_tag="ACHK49_004584"
     tRNA            2864..2948
                     /locus_tag="ACHK49_004584"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2898..2900,aa:Leu,seq:caa)
     gene            3112..4374
                     /locus_tag="ACHK49_004585"
     CDS             3112..4374
                     /locus_tag="ACHK49_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9265078.1"
                     /translation="MKLNARQVETAKPKEKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPMVTLAQARAKRDEAKKLLVQGIDPKAEQKEAQAENSGAYTFET
                     IAREWHASNKRWSEDHRSRILRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFSEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRIPAKREEIKGVRYSYR
                     GMKMKEEHIVPLSRQAMVLLEQLKQISGDKELLFPGDHDAAKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHTERNNVRAAYIHTSEHLDERRL
                     MVQWWADFLEANRNNSVTPYEFAKFMGK"
BASE COUNT         1139 a         1232 c         1118 g         1120 t
ORIGIN      
        1 acagatcgcg cctgacttac cttcttctcc ctcccagcaa actccccaac atcccgcgta
       61 caatctgatt cgtcacctgg cgagcggcgc ttttggccat tgtctgcacc acaccatctt
      121 tcttcccgcc gcgtggtccg gtagtgccga acaaaatatc cttcaatcca ccaagaatgc
      181 cgtcatctac cgctatctct ttccctttcg ctggaggatt attttgctgt tcaatactcg
      241 cctgtacgcc tttttgcaac atctcatagg cggattcccg gtccacgtca tcctcgtact
      301 tgccatacac cggggagtgg ttaatcaagc cattgcgctc atcttccgtc accggtccca
      361 tccgcgaaca aggcgcaatc accatcgccc gttccaccac agaaggactt ccttttgcat
      421 cgagaaacga gattaacgcc tcgccggtcc ccagttcctg gattgccttt tcggtatcaa
      481 acgccggatt ggcccgcatg gtttgcgccg cagcttttac cgctttctga tcttttggcg
      541 taaaagcccg caaagcgtgc tgaacgcgat taccgagctg gccgagcaca ttatccggaa
      601 tatcagacgg gttttgcgaa acgaaccaga cgcccacgcc ttttgagcga ataagcctta
      661 tcacctgctc aatcttatcc agcagtacct gcggtgcatc gttaaacagc agatgtgctt
      721 cgtcgaagaa aaacaccagt tttggcttct ccagatcgcc cgcttccggc aattgttcat
      781 acaactctga aatcatccac agcaggctgg cggcgtacag tttcggcatc tgataaagct
      841 tctcggcgct gaggatatta ataacgcctt taccgttggc atcggtgcgc atcaagtctt
      901 tgatatccag catcggctca ccaaagaagt gtgctgcgcc ttgctgttcc agcgacaaca
      961 gcccgcgctg gatggcacca accgatgcgc tactgatatt tccgtactga ttctggaaag
     1021 atttggcgtt atcgccgatg tactgggtaa tcgcccgcag atctttaaag tcgagcagta
     1081 acagcccctg atcgtcagca attcggaaga tgatattcag cacgccagat tgcacatcgt
     1141 tgagattcaa cagccgcgcc agcaacagcg gccccagatc cgaaaccgtc gcccgcaccg
     1201 gatggccttt ctcgccaaag atatcccaca ccaccaccgg attagcatgc ggttgccagt
     1261 catttacgcc gatattttta agccttgcga gcagtttttc cgacgccgtt cctgcctgcg
     1321 cgataccggt cagatcgcct ttcacatcgg ccataaacac cggcacgccg atttccgata
     1381 gagactccgc cagtttttgc agcgtgacag ttttacccgt ccccgttgcg ccagtaatca
     1441 gcccgtgacg gttagccatt cccggcagta aaaacagttc tgtgtccggc gtgcgggcaa
     1501 ttaacagggg ttcgctcatg agatttcctc cagtttcctg cctggagtat aggcaacggc
     1561 tgtgcactct tcaccgctaa attcagcaga ttgcgagacg ccttccaaag agatttaagt
     1621 gccgggcgac aggctcccgg caaagggatc agaagtcggc tttcaggacc acgcggtagc
     1681 gcgctttacc atcgcgtaca tgctggatgg cgtcgttaat tttcgacatc gggaacagtt
     1741 cggtggtcgg cgcaaccttg ctgcgggcgg caaaacgcat cagcttacgc agctcataag
     1801 gcgtgccggt agcagaacca gagatgctgc ggtcgcccgc aattaacgta aaggccggaa
     1861 cagacagcgg cgtgagaacc gcaccgaccg tatggaaatt accgccatag gtcagcgcct
     1921 caaaataagg ctgccagtcg aggctgacgt tcacagtatt gataatgaga tcaaactgcc
     1981 ccgacagggc tttcagtgcc tgcggatcgc ggctattcac cactttatcg gtacccatcg
     2041 ccagtacttc ctgctctttc gccggattag aactaaaggc cgtcacctcg catcccattg
     2101 cgtgcagaag ttttatagcg atatgcccca gcccgccaat accaattacc ccaacgcggc
     2161 tggtagcagt gatatggtgc atcaacagtg gtttaaagac cgtgataccg ccgcacaaca
     2221 gcggcccggc ggactcgata tcaatatttt ctggcagtgg aatcacccat tgccagtccg
     2281 cacgcaactt ctcggcaaag ccaccgcgat tcataatcgt cggcaccgca ccttgctcgc
     2341 agttgatctg attaccgcta atacaggtgt cgcagtgacc acagctacgc gccgtccagc
     2401 caatcccgac acgctgaccg acctgcaaac ctttatcctg cgcggcgctc ccgagtgcca
     2461 ccacgcgccc aatcacctca tgcccggcaa ccagcggata ttgtgaaaat ccccattcgt
     2521 tatcgatcat cgacagatcg gaatgacaaa tcccgcagta atccacctgc acttcaacat
     2581 cttgtggctt cagctcaccg ggatcgtact cataaacttc cagttcgccg cccgcttctt
     2641 ttgcggcata gctttttatc atcgacattt ttgggtcctt ctctggtgtt gtttgggaag
     2701 tgtagagcat ggcagggcgg ggtgcctgga gtgtgacaaa ggttacatat cgctgagtgt
     2761 aatgctgaaa atttcagcac ttagcgaggt gggtgcaagc tggcgcttgc atggtggcgt
     2821 gcgacaggta taatccacaa cgttttccgc atacctcttc agtgccgaag tggcgaaatc
     2881 ggtagacgca gttgattcaa aatcaaccgt agaaatacgt gccggttcga gtccggcctt
     2941 cggcaccatt agtacttcca agaccatccg agaaaatcca gcaaaccctt taaaatcaat
     3001 gcctgtagcg gtttttctgt cctgagctgt ccgagctagt ccgttgaaat ccggatgcta
     3061 ttgggggcat aattgggggc atcttaactt cgattagaaa tgtgccccca aatgaagctc
     3121 aacgccagac aggtcgagac agcaaagcca aaagagaaaa cctacaaaat ggccgatggc
     3181 ggcggtttgt atctcgaggt ttcggctaag ggttctaaat actggcgcat gaaatacaga
     3241 cgcccctctg acaaaaaaga ggatcgcctc gcttttggtg tttggcccat ggtgacactt
     3301 gctcaggcaa gagcaaagcg tgacgaggct aaaaagctct tagtgcaggg cattgaccca
     3361 aaagccgagc agaaagaagc tcaggctgag aattcgggcg catacacttt cgaaacaatt
     3421 gctcgtgaat ggcatgccag taataagcgc tggagtgaag accatcgatc acgcattctg
     3481 cgctatcttg agctttatat ttttcctcat atcggttcgt ccgacattcg gcagcttaaa
     3541 accagccacc tgttagcccc gattaaaaaa gttgatgcca gtggcaaaca cgatgtcgct
     3601 cagcgcctgc aacagcgcgt cacggccatt atgcgttatg ccgtacagaa cgattacatc
     3661 gactctaatc cagccagtga catggccggt gcgttatcta caaccaaagc acgacactat
     3721 ccagctttac cctctagccg cttctctgag tttcttgcac gtcttgctgc atatcgtggt
     3781 cgtgtaatga caaggatcgc ggtcgagctt tccttactaa cttttgtacg ttccagtgag
     3841 ttacgtttcg cgcgatggga tgagttcgac ttcgataaat ctctttggcg tatacctgca
     3901 aagcgagaag aaattaaagg cgtgcgttac tcgtatcgtg gcatgaagat gaaagaggag
     3961 catatcgttc ctcttagtcg acaggcgatg gttttgttag agcaactcaa gcagattagt
     4021 ggcgataaag agttactttt tccaggggat cacgacgcgg ccaaagttat gagtgagaac
     4081 acagtaaata gcgccttgcg tgcgatgggg tatgacacga aaactgaggt ctgtgggcat
     4141 ggatttagga cgatggcgcg tggcgcgctt ggtgaatcag gcttatggag cgatgacgcg
     4201 atagaacggc agttgagtca tacagagcgt aacaatgtgc gtgcagcata tattcatacc
     4261 tctgagcatt tagacgaacg tagattaatg gttcaatggt gggctgattt tcttgaggcc
     4321 aatcgaaata actctgttac accttatgaa tttgctaaat tcatggggaa ataatgtaac
     4381 tgaaatatta tacattaact aatttggtaa ttgtgtgaga gaattatata ataataaatt
     4441 gaaatgctct tttaaaatgc gggaaaacct caaccaaggg gcattatttt agagcgaggt
     4501 gcaatggcca ttctgagatg atagtgaaaa aacaaaaagt gttcaaacgg tatggctttt
     4561 gttatagaaa ccagccgact aactctgttc attacagttt tagtcggtg
//
LOCUS       DAZGLJ010000292         4608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000292 DAZGLJ010000000
VERSION     DAZGLJ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.292"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            2..1021
                     /gene="tsuA"
                     /locus_tag="ACHK49_004586"
     CDS             2..1021
                     /gene="tsuA"
                     /locus_tag="ACHK49_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO9265079.1"
                     /translation="MLGFVMQRGRFCLTGGFRDMYIAKNNRMFYALLIAISVQSVGVF
                     ALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRAGEGLIGSWIALFT
                     YMVMSAVMRSPHASGLNQTLQHYTTEHNSIADTFNLSVWPLVAVLLVITLWVVMKELK
                     KPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLGITS
                     PTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRAADAQTTLRSGLGG
                     VLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKARLA
                     TAAAN"
     gene            1035..1262
                     /gene="tsuB"
                     /locus_tag="ACHK49_004587"
     CDS             1035..1262
                     /gene="tsuB"
                     /locus_tag="ACHK49_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO9265080.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1305..2732)
                     /gene="sbcB"
                     /locus_tag="ACHK49_004588"
     CDS             complement(1305..2732)
                     /gene="sbcB"
                     /locus_tag="ACHK49_004588"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO9265081.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQETRAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFVEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            2941..4107
                     /gene="dacD"
                     /locus_tag="ACHK49_004589"
     CDS             2941..4107
                     /gene="dacD"
                     /locus_tag="ACHK49_004589"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO9265082.1"
                     /translation="MKRRLIIAASLFIFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRTIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHDEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            4225..>4608
                     /gene="sbmC"
                     /locus_tag="ACHK49_004590"
     CDS             4225..>4608
                     /gene="sbmC"
                     /locus_tag="ACHK49_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO9265083.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVFYDNPDETPAEKLRCDIVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPK"
BASE COUNT         1116 a         1046 c         1216 g         1230 t
ORIGIN      
        1 tctgctggga tttgtcatgc agcgtgggcg tttttgcctg acaggtggtt ttcgcgatat
       61 gtatatcgcg aaaaataatc gcatgtttta cgccttgctg attgcaattt cggtacaaag
      121 cgtgggggtt tttgcgttaa ttcaggcggg cctactgact tacgaagccg gggcgttccc
      181 gtggcttggt actgttatag gtgggtatct tttcgggctg ggaattgttc tggcgggtgg
      241 atgtgccacc gggacctggt atcgcgcggg tgagggattg atcggcagtt ggatcgcgct
      301 tttcacttat atggtgatga gtgcggtgat gcgttctcca cacgccagtg gtttaaatca
      361 aaccttgcag cactacacta ctgaacataa ctctattgct gatactttta atttgtctgt
      421 gtggccgttg gttgccgttt tgctggtgat aacgctctgg gtggtgatga aagagttgaa
      481 gaagccaaaa ctcaaagtcg cgaccttacc gccgcgccga accgggatcg ctcatattct
      541 gtttgaaaaa cgctggcatc cctttgtcac tgctgtactc atcggtttga ttgcgctttt
      601 agcttggccc ctgagtgaag caaccggacg catgtttggg ttaggaatca cttccccaac
      661 ggccaatatt ctgcaatttc tggtcgcggg tgacgtgaaa tacattaact ggggcgtttt
      721 cctggtgtta gggatcttcg tggggtcatt tattgcagcc aaagcgagcc gtgagttccg
      781 cgttcgcgct gctgatgcac aaacaacatt acgtagcggg ttaggtggtg tactgatggg
      841 cttcggtgcc agcattgcag gtggttgctc tatcggtaat ggactggtca tgactgcaat
      901 gatgacctgg cagggctgga ttggccttgt atttatgatt ctcggagtct ggactgcgtc
      961 ctggcttgtg tatgttcgac cgcagcgtaa ggcacgactg gcaactgctg cggcaaatta
     1021 agtaaggtgt aaagatggcg attaaaaagc tggatgtggt gacgcaggtc tgtccgtttc
     1081 cactcataga agcaaaagca gcactggcag aaatggccag cggcgatgag ttggtaattg
     1141 agtttgattg cacccaggca actgaagcaa tcccacagtg ggcggcagaa gaaggacatg
     1201 ccatcaccga ttatcagcag attggcgatg cagcgtggag catcaccgtt caaaaagcct
     1261 gactcaaaat tgtcctccgc cgtaccagcg gcggaggctt cacattagac aatctcttcc
     1321 gcgtactgcc aaagcgcttt taacagcgcc actttttctt tatcgtcggc atactgctgc
     1381 gccagcatct gaatctcttc tgcatagcct tgtaaaaact ctggcgtgaa gacctggcgg
     1441 cggtgctcca gccagcgttg ctgctcggca taatccagcg tccccgggaa gttgcgcgcc
     1501 cgataattga acaacagctt ttcaattcgt ttatcaacaa aagtgatatc cagtgccggt
     1561 aaattacgcg gctcggtttc cagcacaatt ttcattgctg cacgatcggc gtcactgaaa
     1621 aagccgttat aaagctgtgc atccacatta tctgaaggtg taaacggttc ggcttccacg
     1681 aatatcgcta ccactttttc acgcacttgc ggattttcac gcagaatttt taggttatcg
     1741 aggcaatgct gacgattaat tcccagtcgg tcggcatctt ccgggcgtag cgtattcgcc
     1801 tgggccagta ccggacattt attgatatgt accagcttaa ccggaacggc ggcgttatcg
     1861 ccaagatcgg cttttgcggt atataaacgc tcgcgcaatg tgtcgctatc cagctccagt
     1921 aatggcgaaa tgtctcctgc caaatccacc ataattacgg cattgcgatt ttcaggatgc
     1981 cacgccagcg gtgccaccca gcttgtattg ccgcgccatg caccaaacat tccggaaacg
     2041 tgaaccaggg gtttcatctg aggaacatca atcaacgcca ttagtttgtg tttattacga
     2101 tgggtaaaga gataatcaaa caggcgtggc tgacgcgttt ttaccagttt tgccatcgca
     2161 atagtggcgt acacatcagc catcgcatcg tgggcgttgc tatgttcaat accattcgct
     2221 ttggttaaat gctcaaggcg aaagctcggt agaccgtcat cattttcagg ccaatttatt
     2281 ccttccgggc gcagggcata acaggcacgc ataacatcca gtaaatccca gcgcgagtta
     2341 tcatgctgcc agctccaggc gtaaggatcg tagaaattac gataaaaaac gttgcgtgtg
     2401 acttcgtcgt cgaaacgcac attgttgtag cccagaatac aggtcttcgg tacggtaaaa
     2461 agcgagtgaa tacgggcggc aaacgcggct tcgttttctc ctttcgctcg tgtctcctgc
     2521 ggggtaatac cggtaattaa tactgctcca ggctggggta aatagtcatc cgcgggcttg
     2581 cagtaaaaga cttcgggttc gccgatgaca ttgaattcgt catcggtgcg aatggctgcg
     2641 aactgtgcag ggcgatctaa cgcggggtgc gtgccaaagg tttcgtaatc gtgaaacaaa
     2701 aaggtagatt gttgcttacc gtcattcatc attaggttaa atccgttatt tctgctgtct
     2761 gccagagtat caaatatcac cgtgctaatc agctttagcg cgacaatttg acagcgagtg
     2821 acaacagatc atgtcagata aaaatgagag ggtagtcaca ttttcttgca ctttattcca
     2881 gccagttcat aagtatttcc gtaaaaagaa cagctatttg aaactcctga gggtttgctg
     2941 ttgaaacgcc gtcttattat tgctgcttct ttgttcattt ttaacttatc gtctggtttt
     3001 gcggcggaaa acattccttt ttcacctcag cctccagaga ttcatgccgg ctcctgggta
     3061 ctgatggatt acaccaccgg tcagatcctc accgcgggta atgagcatca acagcgcaat
     3121 cccgccagcc tgacaaagct gatgacgggc tatgtcgtgg atcgcactat cgatagtcat
     3181 cgcattacgc cagacgatat tgtcaccgtg gggcgcgatg cgtgggcgaa agataatcca
     3241 gtgtttgtcg gttcttcact gatgtttttg aaagagggcg atcgcgtatc ggtacgtgat
     3301 ttaagccgtg gtttaattgt ggattccgga aatgacgctt gtgttgcact ggctgactat
     3361 attgccggtg ggcaacggca gtttgttgaa atgatgaaca actatgccga gaagctgcat
     3421 ctcaaggata cgcattttga aacagtgcat ggtctggatg caccaggtca gcatagctcg
     3481 gcttatgatt tagctgtgct ttctcgcgct atcatccacg acgagcccga gttttatcat
     3541 atgtacagtg agaaaagtct cacctggaac ggtatcaccc agcaaaaccg taacgggtta
     3601 ttgtgggata aaaccatgaa tgttgacggc ctgaaaacgg gtcatacttc tggtgccggg
     3661 tttaatctca ttgcttcggc tgtcgatggg cagcgtcgtc tcattgcagt ggtaatgggg
     3721 gctgacagtg caaaaggtcg tgaggaagag gcaagaaaat tactgcgttg ggggcaacaa
     3781 aactttacta cggtgcaaat tttgcaccgt gggaaaaagg tcggtacgga acgcatctgg
     3841 tatggcgata aagaaaatat cgacctggga acggaacaag agttctggat ggtgctaccg
     3901 aaagccgaaa ttccacatat caaagccaaa tatacccttg atggtaaaga gctcaccgcg
     3961 ccaattagcg cccatcagcg ggtaggggaa attgaacttt acgaccgtga taaacaggtg
     4021 gcgcactggc cgctggttac cctggaatct gtcggggaag gcagcatgtt ttctcgcctg
     4081 agtgattatt tccaccataa ggcctgacct ttcttttgca gcagactggc aggagtgcga
     4141 gtctgctcgc ataatcaaca ctcattcctt gtggttttaa tatcgcaatt atactgtata
     4201 taaaaacagt gttaatggga cgtcatgaac tacgagatta agcaggaaga taaacgtacc
     4261 gttgcaggtt tccatctcgt tggcccgtgg gaacagacgg taaagaaagg ctttgagcag
     4321 ttgatgatgt gggtagatag caaaaatatt gtgccgaagg agtgggttgc tgtcttttac
     4381 gacaatccag atgaaacacc cgccgaaaaa ttacgctgcg acatcgtcgt gacggtgccg
     4441 aataacttta cgctccccga aaacagtgag ggcgtcattc tgacagaaat ttcaggtggt
     4501 cagtatgcgg ttgcggtggc tcgtgtagtc ggtgatgatt ttgctaaacc ctggtatcag
     4561 ttctttaata gcctcttgca ggacagtgct tatgaaatgt taccaaag
//
LOCUS       DAZGLJ010000293         4598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000293 DAZGLJ010000000
VERSION     DAZGLJ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.293"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1792
                     /locus_tag="ACHK49_004591"
     CDS             <1..1792
                     /locus_tag="ACHK49_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265084.1"
                     /translation="GELREFELADGNWLLNNAWYERNMAGFNEQLKENLSFTPDELKT
                     LFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICASCGLTEVHHA
                     PENHKPDPEWYCTSLCRETETLCQEIYERPYNSFISDATANGLILMKLPETWSTNEKM
                     FASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEIQTKYCSTAAR
                     SVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVPGVTDPAEASR
                     LIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTASVFWLSTGDRD
                     AALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPTVRLALQKGTG
                     AGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGTQFIRNLAVASSGVAGGA
                     VGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEEDRVKILAMIQE
                     QVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNMLIKPLVVSII
                     RQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            1868..2323
                     /locus_tag="ACHK49_004592"
     CDS             1868..2323
                     /locus_tag="ACHK49_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO9265085.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            2402..2635
                     /locus_tag="ACHK49_004593"
     CDS             2402..2635
                     /locus_tag="ACHK49_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO9265086.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            2735..3553
                     /locus_tag="ACHK49_004594"
     CDS             2735..3553
                     /locus_tag="ACHK49_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO9265087.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRVLWVMAETLLESLR"
     gene            3608..4093
                     /locus_tag="ACHK49_004595"
     CDS             3608..4093
                     /locus_tag="ACHK49_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9265088.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            4109..4585
                     /locus_tag="ACHK49_004596"
     CDS             4109..4585
                     /locus_tag="ACHK49_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO9265089.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
BASE COUNT         1123 a         1208 c         1230 g         1037 t
ORIGIN      
        1 gggagagctg agggaatttg aactggcaga cggaaactgg ttactgaaca atgcctggta
       61 tgaaagaaac atggcagggt ttaacgaaca gttgaaagag aacctgtcat tcacacctga
      121 tgaactgaaa acgctcttcc ggaaccgcct gaatttatca ccggaagcga atgacgattt
      181 tctcgatcgt tgcctggacg gcggtgactg gtatcccttt tcagaaggtc gccgttttgt
      241 atcattccat cacgtggatg agcttcgtat ctgtgcctcc tgcgggctga cagaagtaca
      301 tcatgccccg gaaaatcata agccggatcc ggaatggtac tgcacctctc tttgtcgcga
      361 aacagaaaca ctgtgtcagg aaatttatga acgcccttac aacagcttta tttccgatgc
      421 aacggcgaat ggtctgattc tcatgaaact gccggaaacc tggagtacaa atgagaaaat
      481 gtttgcttcc ggagggcagg gacatgggtt tgccgctgaa cggggaaacc atattgtcga
      541 cagagtccgt ctgaaaaacg cacggatcct cggtgataat aatgccagaa atggagcaga
      601 cagactggtc agcggaacag aaatccagac gaaatattgt tcaactgcag cccgtagcgt
      661 cggtgcggca ttcgacggac aaaacggaca gtatcgttac atgggaaata atggccccat
      721 gcaactggaa gtcccccgtg atcagtatgc cggcgctgtt gaaaccatga ggaataagat
      781 ccgcgaaggt aaagtacccg gtgtaaccga tcccgcagaa gcatcccggc tgattcgtcg
      841 gggacatctg acttatactc aggcccgtaa tatcacccgg ttcgggacca tcgaatcggt
      901 cacttatgat attgccgagg gttcggttgt cagtctggcg gccggaggga tcagttttgc
      961 cctgacggca tcggtcttct ggctcagcac cggcgatcgc gatgctgccc tgcagacagc
     1021 tgctgtccag gcaggaaaaa ccttcacccg cacactggct gtctacgtca caacccagca
     1081 actccaccgg ctcagtgttg ttcagggtat gctgaagcac attgattttt cgactgccag
     1141 cccgactgtt cgactggctc ttcagaaggg gaccggtgca ggaaatatca gtgccctgaa
     1201 caaagtgatg aagggtacgc tggtgacatc tctggcactg gtagctgtca caaccggccc
     1261 tgacatgatc aaaatgttgc ggggacggat ctccggtacg cagttcatca ggaatcttgc
     1321 cgtggcatct tccggtgtgg caggtggtgc tgtcgggtca gtggcgggcg gaatactgtt
     1381 cagtccactg ggaccatttg gtgcactgac agggcgtgtg gttggcggtg ttctgggggg
     1441 aatgattgcc tccgctgtat caggaaaaat tgccggagcg ctggttgaag aagatcgcgt
     1501 caaaattctg gcaatgattc aggagcaggt gacatggctt gccggcagtt tcctgctgac
     1561 cggacatgag attgaaaatc tgaacgagaa tctggcccgt gttatcgatc agaatgctct
     1621 ggagatcatt ttcgccgccg gtatacaaca acgggccgcg accaatatgt taatcaaacc
     1681 actggtggtc agtatcatca ggcaacgccc cgtcatggaa tatgatgcat cccatctcgg
     1741 caatatggtt aaccgactgg aagaagcatt acccccggag ttaccggcat aacggctatt
     1801 ctgcgccttt tacgctttat cacctgattt gtcagtgtca tcgtcatgac tcagagaggt
     1861 aaataccatg attcacctgt tcaaaacctg catgattacc gccttcattc tggggttaac
     1921 gtggtctgcc ccactccggg cacaggatca acgttacatc agtatacgca atacagatac
     1981 gatatggctc ccgggaaata tttgtgctta ccagttccgg ctggataatg gcggaaacga
     2041 tgaaggattt ggccccctca ccatcactct gcaactcaaa gacaaatatg gtcagacgct
     2101 ggtgaccaga aaaatggaaa cggaagcttt tggtgacagt aatgccacgc gaaccacaga
     2161 cgcatttctg gaaacggagt gcgtggaaaa tgtcgccaca accgaaatca tcaaagcaac
     2221 tgaagaaagt aacggccatc gtgtcagtct gccgttatcg gttttcaatc cccaggacta
     2281 ccatccactg ctgattaccg tttccggaaa aaacgttaac tgatccctga agtcagtttc
     2341 atgcttcagc ctctgtcaca acgcgtggca gaggcttttt atttatcagg agaaaacaga
     2401 tatgccgggt tacacagagt atgtcctggc tgaaggatct ttctcatacg ggcaggcggt
     2461 tgctgtcata accgcttacc gcaatgtgtt tatccaggat gacccgggaa tgcatttccg
     2521 tcgggttatc cgcaacgcgg aaggtcagcg acgctggcgc tgccggaatt ctgaacccga
     2581 tgccggaaag gtgcttaata cgcggctcgc cagtgacggt cttctcagac aataaacgtc
     2641 attcatttca cccatcaggc cgcgtcttct ccgggagacg tggctttttc atttattaca
     2701 ccagtaaatc ttaaccaccg ataaggagca aattatgcga ctagccagtc gttttggtta
     2761 tgcgaaccag atacgccgtg accgtccgct gacacacgaa gaactgatgc actatgtgcc
     2821 ggggattttc ggggaagata aacacacgtc ccgaagccag aactatacgt acatccccac
     2881 catcaccgta ctggaaagcc tgcagcggga aggctttcag ccattcttcg cctgccagac
     2941 ccgtgtgcgc gacccgggcc gccggggata cacaaaacac atgctgcgtc tgcggcgggc
     3001 cggagagata aacggagaac atgtccctga aattattctg ctcaactctc atgacggtac
     3061 ctccagctac cagatgctgc cgggttactt caggttcgtc tgccagaacg gatgcgtctg
     3121 tggtcagtct ctgggggaag tgcgtgttcc gcaccgggga aatgtggtgg acagagtcat
     3181 tgaaggggct tacgaggtgg tgggcgtgtt tgaccggatt gaggaaaagc gtgatgccat
     3241 gcagtcgctg attctgccgc caccggcacg ccaggcgctg gcacaggcgg cactgactta
     3301 ccgttatggt gacgaacatc agcccgtcac caccgccgac attctgacgc cacgacgccg
     3361 ggaggattac ggtaaggacc tgtggagtgc ttatcagacc attcaggaga atatgctgaa
     3421 aggcggaatt tcaggtcgca gcgctaaagg aaaacgtatc catacccgtg ccattcacag
     3481 cattgacact gacattaagc tcaaccgcgt attgtgggtg atggcggaaa ccctgctgga
     3541 gagcctgcgc tgattcagcc attaccctga aaaccacgca tttatttgtc ctttaaggag
     3601 tcatttcatg aaaaccattt cacataattc caccacatct tctgtttccg taaccgctgc
     3661 atcagggaat gaccagcctc agttggttgc caccctcgtc cctgatgaac agcgcatcag
     3721 cttctggccg cagcactttg gcctcattcc acagtgggtg accctggagc cccgtgtctt
     3781 cggctggatg gaccgtctgt gtgaagacta ctgcggtggt atctggaatc tgtacaccct
     3841 gaacaacggc ggggcattta tggcacccga accggatgac gatgatgacg aaacatgggt
     3901 actgttcaat gccatgaacg gtaaccgcgc tgaaatgagt ccggaagctg ccggcattgc
     3961 tgcctgtctg atgacgtaca gtcatcatgc ctgtcgtacg gagtgttatg ccatgacggt
     4021 ccattattac cggctgcggg attacgccct gcagcatccg gaatgcagcg ccattatgcg
     4081 catcatcgac tgaatggaga gaagtacaat gcaacagctt tcctttctgc ccggagagat
     4141 gacgcccggc gagcgcagcc tcattcaacg ggccctgaaa accctggacc gccatcttca
     4201 tgaacccggc gtggccttca cctccacccg tgcggcacga gaatggctga ttctgaacat
     4261 ggcgggactg gagcgtgaag agttccgggt gctgtatctg aacaaccaga atcagctgat
     4321 tgccggtgaa accctcttca ccggcaccat caaccgcacg gaagtccatc cccgtgaagt
     4381 gattaaacgc gccctgtatc acaatgccgc tgctgtggtg ctggcacaca atcacccgtc
     4441 cggtgaagtc acacccagca aggcagaccg ccttatcacg gaacgtctgg tacaggcact
     4501 gggcctggtg gatatccggg tgccggacca tctgatagtc ggtggcagcc aggttttctc
     4561 ctttgccgaa catggtctgc tttaacccgt cacaacca
//
LOCUS       DAZGLJ010000294         4523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000294 DAZGLJ010000000
VERSION     DAZGLJ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.294"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            268..4520
                     /gene="fdeC"
                     /locus_tag="ACHK49_004597"
                     /pseudo
     CDS             268..4520
                     /gene="fdeC"
                     /locus_tag="ACHK49_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
BASE COUNT         1191 a         1062 c         1266 g         1004 t
ORIGIN      
        1 agtaagaaat ataacttagg tatctattta atgacttgca caaaaagcta aattttcccc
       61 cataaataaa aatataatcc cgcgcccaac cacctgatga gtggctacag gcactggata
      121 tattaggtgg cggtgcactt tcttacataa aggtatttcc ttttctgcgg aaaaggaaat
      181 cgggaaatcc ccggtttttc tgacaagcag acgccattat ttgtgtctgc ctatgttcgt
      241 taattcgttc atcaggaaat tatctcaatg tcacattata aaacaggtca taaacaacca
      301 cgatttcgtt attcagttct gacccgctgc gtggcgtggg caaatatctc tgttcaggtt
      361 ctttttccac tcgctgtcac ctttacccca gtaatggcgg cacgtgcgca gcatgcggtt
      421 cagccacggt tgagcatggg aaatactacg gtaactgctg ataataacgt ggagaaaaat
      481 gtcgcgtcgt ttgccgcaaa tgccgggaca tttttaagca gtcagccaga tagcgatgcg
      541 acacgtaact ttattaccgg aatggccaca gctaaagcta accaggaaat acaggagtgg
      601 ctcgggaaat atggtactgc gcgcgtcaaa ctgaatgtcg ataaagattt ctcgctgaag
      661 gattcttcgc tggaaatgct ttatccgatt tatgatacgc caacaaatat gttgttcact
      721 cagggagcaa tacatcgtac cgacgatcgt actcagtcaa atattggttt tggctggcgt
      781 catttttcag gaaatgactg gatggcgggg gtgaacacct ttatcgacca tgatttatcc
      841 cgtagtcata cccgcattgg tgttggtgcg gaatactggc acgattatct gaaactgagc
      901 gccaatggtt atatccgggc ttctggctgg aaaaaatcgc cggatgttga ggattatcag
      961 gaacgcccgg cgaatggctg ggatattcgt gctgagggct atttacccgc ctggccgcag
     1021 cttggcgcaa gcctgatgta tgaacagtat tatggcgatg aagtcgggct gtttggtaaa
     1081 gataagcgcc agaaagaccc gcatgctatt tctgccgagg tgacctatac gccagtgcct
     1141 cttctgacac tgagcgccgg gcataagcag ggcaagagtg gtgagaatga cactcgcttt
     1201 ggcctggaag ttaattatcg gattggcgaa cctctggcga aacaactcga tacagacagc
     1261 attcgcgagc gtcgggtact ggcaggcagc cgctatgacc tggttgagcg taataacaac
     1321 atcgttcttg agtaccgcaa atctgaagtg atccgtattg ctctgcctga acgtattgaa
     1381 ggtaagggtg gtcagacact ttccctgggg cttgtggtca gcaaagcaac tcacggactg
     1441 aaaaatgtgc agtgggaagc gccgtcatta ctggctgagg gtggcaaaat taccggtcag
     1501 ggtagtcagt ggcaagtaac gctcccggct tatcgtccag gcaaagacaa ttattatgcg
     1561 atttctgcgg ttgcctacga taacaaaggc aatgcctcaa aacgcgtgca gacagaggtg
     1621 gtcattaccg gagcaggtat gagcgccgat cgcacggcgt taacgcttga cggtcagagc
     1681 cgtattcaaa tgcttgctaa cggtaatgag caaagaccgc tggtgctgtc tctgcgcgac
     1741 gccgaggggc agccagtcac gggcatgaaa gatcagatca agactgaact agccttcaaa
     1801 ccggctggaa atattgtgac tcgttcccta aaggccacta aatcacaggc aaagccaaca
     1861 ctgggtgagt tcaccgaaac tgaagcaggg gtgtatcagt ctgtctttac taccggaacg
     1921 cagtcaggtg aggcaacgat tactgttagc gttgatggca tgagcaaaac cgtcactgca
     1981 gaactgcggg ccacgatgat ggatgtggca aactccaccc tgagcgctaa cgagccgtca
     2041 ggtgatgtgg ttgctgatgg tcagcaagcc tatacgttga cgttgactgc ggtggactcc
     2101 gagggtaatc cggtgacggg agaagccagc cgcttgcgat ttgttccgca agacactaat
     2161 ggtgtaaccg ttggtgccat ttcggaaata aaaccaggcg tttacagcgc cacggtttct
     2221 tcaacccgtg ccggaaacgt tgttgtgcgt gctttcagcg agcagtatca gctgggcaca
     2281 ttacaacaaa cgctgaagtt tgttgccggg ccgcttgatg cagcacattc gtccatcaca
     2341 ctgaatcctg ataaaccggt ggttggcggt acagttacgg caatctggac ggcaaaagat
     2401 gctaatgaca accctgtaac tggtctcaat ccggatgcac cgtcattatc gggcgcagct
     2461 gctgtaggtt ctacggcatc aggctggacg gataatggcg acgggacctg gactgcgcag
     2521 atttctctcg gcactacggc gggtgaatta gaggttatgc cgaagctaaa tggacaggat
     2581 gcggcagcga atgcggctaa agtaacgtgg tggctgatgc gttatcttca ggccagtcga
     2641 cagtgtctgt agccgcagat cgcgtaaaag caggcgaaag cacaaccgtg acgcttattg
     2701 caaaagatgc acatggcaac gctatcagtg gtctttccct gtcggcaagc ctgacgggtg
     2761 ctgcgtctga aggggcgact gtttctggtt ggaccgaaaa aggtgatggt tcctatgtcg
     2821 ctacgctgac aacaggtgga aagacgggtg agcttctcgt catgccgcta ttcaacggcc
     2881 agccagcagc caccgaagcc gcgcagttga ctgtcattgc gggggagatg tcatcagcga
     2941 actctacgct tgttgctgac aataaggctc cgaccgtcaa aacgacgacg aaactcacct
     3001 tcaccgtgaa ggatgcgtac gggaaccttg tcaccgggct gaagccagat gcaccgcagt
     3061 ttagtggtgc cgccagcacg gggacagagc gaccttcaac aggagactgg acagaaacaa
     3121 gtaatggggt ctacgtggcg accttaacgc tgggatctgc cgcgggccag ttgtctgtga
     3181 tgccgcgagt gaacggccaa aatgccgttg ctcagccact ggtgctgaat gttgctggtg
     3241 acgcatctaa ggctgagatt cgtgatatga cggtgaaggt tgataaccag ctggctaatg
     3301 gacaatcgac taaccaggta accctgaccg ttgtggacac ctatggtaac ccgttgcagg
     3361 gacaaaatgt gacgctgact ctgccgaaag gtgtgaccag caagacgggg aatacggtaa
     3421 caaccgatgc ggcaggtaaa gccgacattg agctgatgtc aacggttgcc ggggaacaca
     3481 gcatcacggc ctcagtgaat aatgctcaga agacggtcac ggtgaaattc aacgcggatg
     3541 ccagcaccgg tcaggcaaac ctgcaggtag acaccgctgc tcaaaaagtg gcaaacggca
     3601 aagatgcctt tacgctgacg gcgaacgttg aggataaaaa tggtaaccct gttccaggga
     3661 gcctggtgac ctttaatctg ccccggggtg tcaagccgct tacaggcgat aatgtctggg
     3721 tgaaagccaa cgatgagggg aaagccgagt tgcaggtggt ttcagtgact gccggaacgt
     3781 atgagatcac ggcatcggca gggaatagcc agccttcgaa tacgcagact ataacgtttg
     3841 tagccgataa ggctaccgca accgtctccg gtattgaggt gatgggcaac tatgcgctgg
     3901 cggacggcaa agccaaacaa acgtataaag ttacggtgac tgatgccaat aacaatttgg
     3961 tgaaagatag cgaagtgacg ctgactgcca gcccggcttc gttaaacctg gaaccgaatg
     4021 gcactgcgac aacgaatgag caagggcagg ctattttcac cgctaccact actgttgcgg
     4081 cgacatacac actcaaggcg caagtgagtc agaccaacgg tcaggtatca acgaaaactg
     4141 ccgaatctaa attcgttgcg gatgataaaa acgcggtact caccgcatca tctgatatgc
     4201 aatctctggt ggcggatggg aaatcgactg cgaagctgga ggtgacactg atgtcggcaa
     4261 acaaccccgt tggcgggaat atgtgggtcg acattcagac gccggaaggg gtgacggaaa
     4321 aggattatca gttcctgccg tcgaaaaatg accatttcgt gagcggaaaa atcacgcgta
     4381 aatttagtac cagcaagcct ggtgtctata cgttcacatt taacgccctg acctatggcg
     4441 ggtacgaaat gaagccagtg acggtgacca ttaccgcggt ggatgccgat acggcaaagg
     4501 acgaggaggc gatgaaataa ttt
//
LOCUS       DAZGLJ010000295         4517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000295 DAZGLJ010000000
VERSION     DAZGLJ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.295"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            137..1234
                     /gene="mltA"
                     /locus_tag="ACHK49_004598"
     CDS             137..1234
                     /gene="mltA"
                     /locus_tag="ACHK49_004598"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO9265090.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1311..2117
                     /gene="tcdA"
                     /locus_tag="ACHK49_004599"
     CDS             1311..2117
                     /gene="tcdA"
                     /locus_tag="ACHK49_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO9265091.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2168..2611)
                     /gene="csdE"
                     /locus_tag="ACHK49_004600"
     CDS             complement(2168..2611)
                     /gene="csdE"
                     /locus_tag="ACHK49_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO9265092.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAIEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2611..3816)
                     /gene="csdA"
                     /locus_tag="ACHK49_004601"
     CDS             complement(2611..3816)
                     /gene="csdA"
                     /locus_tag="ACHK49_004601"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO9265093.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQLGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLNIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4008..4235
                     /gene="ygdI"
                     /locus_tag="ACHK49_004602"
     CDS             4008..4235
                     /gene="ygdI"
                     /locus_tag="ACHK49_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO9265094.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
BASE COUNT         1085 a         1136 c         1221 g         1075 t
ORIGIN      
        1 tgattttgaa caaaaacaaa ccactaaagc ctattttttg tgcaaaattt gcgtcaggga
       61 gaggctgctc atttcattaa acgcggcggt ttgttatctt cgttgcgcct tattttttaa
      121 cctgaagaag agaacaatga aaggacgttg ggtaaagtac cttcttatgg gcacggttgt
      181 ggcaatgctt gccgcctgct cttccaaacc aaccgatcgc ggacagcaat ataaagacgg
      241 gaaatttacc cagcctttct ctctggtgaa ccagccagat gccgttggcg cgccgattaa
      301 cgccggtgat tttgccgagc aaattaacca tatccgtaat tcgtcaccgc gtctgtatgg
      361 caaccagagt aatgtttata acgcggtgca agagtggctg cgcgcaggcg gtgatacccg
      421 caatatgcgc cagttcggca ttgatgcctg gcagatggaa ggtgccgaca actatggtaa
      481 cgtgcagttt acaggctatt acacgccggt aattcaggcg cgccataccc gccagggcga
      541 gttccagtat cctatttacc gtatgccgcc aaaacgtggt cgtctgccgt ctcgtgcgga
      601 gatctacgca ggggcgctga gtgataaata tattctcgct tacagcaact ccctgatgga
      661 taacttcatt atggatgtgc agggtagcgg gtatatcgac tttggtgatg gcagtccgct
      721 taactttttc agctatgcag ggaaaaacgg tcatgcctat cgcagcattg gtaaggtgct
      781 gatcgaccgt ggcgaagtga aaaaagaaga tatgtcgatg caggcgattc gtcattgggg
      841 cgaaacacac agtgaagccg aggttcgcga gctgctggaa cagaacccgt ctttcgtttt
      901 ctttaaaccg caatcttttg caccggtgaa aggggcaagt gcggtgccgc tggttggccg
      961 tgcttctgtt gcctccgatc gttcaattat tccgccaggt actaccttgc tggcagaagt
     1021 gccgctgctg gataataacg gcaaatttaa cggtcagtac gaattgcgtc tgatggtggc
     1081 gctggatgtc ggtggcgcaa tcaaaggcca acacttcgat atctatcaag ggatcgggcc
     1141 ggaagccgga catcgcgcag gttggtacaa ccactatgga cgtgtctggg tgctgaaaac
     1201 cgccccgggc gcaggtaacg tctttagcgg ctgatgtgtt attctgtata ccgtttttac
     1261 ggatacgaca gggtgaggga taacctcacc ctttttaatt ctgaggtgct atgtctgtgg
     1321 taattagtga tgcatggcgt cagcgttttg gtggcacagc gcgtctgtat ggtgaaaaag
     1381 cgttgcaact gtttgctgac gcgcatattt gtgtggttgg tatcggtggt gtcggttcct
     1441 gggcggcgga agcgctggcg cgcacgggga ttggcgcaat cacgcttatc gatatggatg
     1501 atgtgtgcgt caccaatacc aatcggcaaa ttcacgctct gcgcgataac gtcgggctgg
     1561 caaaagcgga agtcatggcg gagcgtattc gccagattaa cccggagtgc cgtgtaacgg
     1621 tggtggatga tttcgtgacg ccggataacg tagcgcagta tatgagcgtg gggtattcgt
     1681 acgtgattga tgccattgat agtgtacggc ccaaagcggc gctgattgct tattgtcggc
     1741 gcaataaaat cccgctggtc acgaccggtg gcgcgggtgg gcagattgac ccgacgcaga
     1801 ttcaggttac cgatctggcg aaaacgattc aggatccgct ggcggcgaag ttgcgcgagc
     1861 gcctgaaaag cgattttggc gtagtgaaaa acagtaaagg taagctcggc gtcgattgcg
     1921 tgttttctac tgaagcgctg gtgtacccgc agtcagacgg tactgtgtgt gcgatgaaag
     1981 ccacggcaga agggccgaag cggatggatt gtgcatctgg atttggcgcg gcaacgatgg
     2041 tgaccgccac ctttgggttt gttgcggttt ctcatgcgct gaagaagatg atggcgaaag
     2101 cggcgcgtca gggttaaaca atcaggtcgg atgcgacgcg aacgtcttat ccgacctggt
     2161 tctgtgctta aacctgcttc gtcgcagcga taatcgcctc gcttaacgca ttcaacccct
     2221 ggctgcgtga ggcgctaagc tgcccacgca atcccagctc atcaaacaat gccagcggcg
     2281 actgtgcctg caattcggcg gcggttttcc cctcaatggc agtcaacagc accgccagca
     2341 ggccgcgcac tatacgtcct tcgctgtcgc caaagaagtg cattttaccg ttttcagcca
     2401 cggtatatcc cagccagacg cggttttcgc atccggcaat ctctttagcc tgcgctttta
     2461 actcgtctgg caaagccgga agctgtttcc ccagcatgat caactggcga tatttatctt
     2521 cccattgcgt caacggtgcg aaggtattgc gtaacgtttc tgcggttacg gttgtgccga
     2581 acggatgtcc ggcgaattgc gggttcgtca ttaatccacc aataattcca gcgcgcggtc
     2641 aacggcattc accagcgcat ccacatcact ctttgtatta tatggcgcaa aagaggcgcg
     2701 cagtgtgccg gttacgccta attctgccag tagcggctga gcgcaatgtt gcccggcccg
     2761 caaggcaata ccgtactccg ccagcagcgt caccatatcg ctgtgatgaa cgccagcaaa
     2821 atcaaaggcc agcaggctgg aatcctggca gcggaatgaa cggaaacctg gacgtttcgc
     2881 cagcgcatct tccgccagtg ttgctaagct acggctccag ttttcggcct ggttgatatc
     2941 gtaatctgcc agccattcca gcgccgcgct taatcctatg acaccagcca catttggcgt
     3001 tcctgcttcc agtttccacg gcgcagattg agtcgtgaag ccgtcaaaac tcacttcgtg
     3061 aaccattttg ccgccgccca gccagggcga catcgcttcc agcagttctg atttgccata
     3121 cagcacgccg atacctgtcg ggccatacag tttgtgacct gaaaaagcat agaaatcaat
     3181 attcagttgc tgaacatccg cggggaaatg caccgccccc tgagcaccat caaccatcac
     3241 caccatcccg gctgaatgag caaaggtaat cgctcgcgcc agatccgggc aaccgccagt
     3301 aacgttcgac atctgaccca acgccagaat ccgactacgg ggagtaatca gttctggcaa
     3361 caaatcgaca tccggcagtc gctgcgcatt aagcggcaat ttcaccactt tggctccagt
     3421 ttgttgggcg accatcagcc aggggacgag gttggcgtgg tgttctgcca cgctgacaat
     3481 aatctcatcg cccagttgca gacgcggacg cgcatagcat tgtgcaacca tgttgatgga
     3541 ttcagtggtg ccgcgcgtcc agacgatagt tttatcatcc ggtgcattca gtaattgcgc
     3601 cactttctct cgtgcagctt cataacgcgc ggtcaggcgt tgggcttcgg caaactggct
     3661 gcgatggacg tttccggcgc tcaggctgta aaactgtcgg gtggcttcaa ccacggcttc
     3721 aggtttaagc gcggtcgcgg cgctgtcgag atagacgccc gcatcctgta gagcgggaaa
     3781 ctgggcgcga aactgcgcgg gattaaaaac gttcatggta ctcctcggct tgatgcaccg
     3841 atcgtgacgc aaaattcact ttgatacaag gagagtgcta accatcagaa ttatctggat
     3901 atcctggcga agccgcgtaa gtaacatatg ctgaatagtg ttaggcgtta attttagcct
     3961 gttaatcaat actattaaga tttattaatc gctttaagga gaagatgatg aaaaagactg
     4021 ccgcaattat ttctgcctgt atgctgactt ttgccctgag cgcctgttcc ggttcgaact
     4081 atgtgatgca caccaatgac ggacgtacca tcgtctctga cggcaaacca cagactgata
     4141 acgataccgg tatgatttcg tataaagacg ctaatggcaa caaacagcag atcaaccgta
     4201 ctgacgtgaa agagatggtc gaactggatc agtaattcac gatcgcaagg taaaaaaaag
     4261 caccgcaatt aggcggtgct acattaatca ctatggacag acagggtaaa tgtacaggaa
     4321 gtgaaaaaag gtagctttgc taccatggtc tgaatcgcag accaattgca aacacaacaa
     4381 cacaacatca caaccgtaag ccaaaagttc accagaacac gcattccgat aaaacttttc
     4441 gttccggctc aggaagtgcc gccactatag gtatttgctg gtagaagctc aacggacaat
     4501 ttataatggc tcagatt
//
LOCUS       DAZGLJ010000296         4495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000296 DAZGLJ010000000
VERSION     DAZGLJ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.296"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..551)
                     /locus_tag="ACHK49_004603"
                     /pseudo
     CDS             complement(1..551)
                     /locus_tag="ACHK49_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
     gene            complement(511..630)
                     /locus_tag="ACHK49_004604"
                     /pseudo
     CDS             complement(511..630)
                     /locus_tag="ACHK49_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            complement(643..2253)
                     /locus_tag="ACHK49_004605"
     CDS             complement(643..2253)
                     /locus_tag="ACHK49_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFO9265095.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSNGSMTLMLKEMSNLINDEGIK"
     gene            complement(2928..3917)
                     /locus_tag="ACHK49_004606"
     CDS             complement(2928..3917)
                     /locus_tag="ACHK49_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265096.1"
                     /translation="MTMTKDSYQFGIEPVRITDTDNIQVNEGLPTNADPQVYALALAK
                     AVKTMLNGVLKSAQDNIPFPIEILPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDAKSSTGFTQGTLQQDALNICDNVVRTINSRMV
                     DVLESSKLLKTVELPVLTGSLTAKADTIMDALYENTEPSFGSEVADYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDIAYYNGEDRGVFMMAKRFTALSFGCFRHDGDHITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(3992..>4495)
                     /locus_tag="ACHK49_004607"
     CDS             complement(3992..>4495)
                     /locus_tag="ACHK49_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265097.1"
                     /translation="SKASALFNNQDWKLSVTDINLYENTVSLDGQFYPLSLAIKTLIP
                     GYLSGLPSTSRESMELLEALAEAGVTIGNFFSNDLMTAYGRRQQNKRAEAERIAKEQR
                     IQAERMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESG
                     GNWMDNL"
BASE COUNT         1173 a         1038 c          968 g         1316 t
ORIGIN      
        1 caatatcaac aagacttctg taacatgttt tactaaatcc aattacaaaa tgaatggacc
       61 aatataatga ttttttgtct aacactggag cgtttccgta ttggcagact gtgactttat
      121 ctaaccaaag attcaattcc cctgttcata ttcttattag tggttgagca gggctcaata
      181 ctccatcaaa tactttacct acgtgcaaac gattatattt tagttcagga tgctttagat
      241 ttaacgggga actggataaa ctgagccatt cccctattaa cctttccgtt tctggcatta
      301 aagccatccc agcgagctca gatgaaatta agggaaatat ttctccaagc aatacccatc
      361 cagaccagct gccactctct tctgctatcg gtaaccattt agcactaaat tcgggcatta
      421 ataatgcaac atcttcgcca ttttctttcc aattttgaaa agtggtttca aacgtatgag
      481 tgtttcgatt tactttcctc agaccgaaca ttaactttca tccttctgat cgttttcgaa
      541 tttgctttca ttttcttgct cctcataagg ctgatgtcgt tgtccttgtc gttcatcttg
      601 ctcagtcaat acccaacgtt gtgctgtaac tcattatgaa aactatttaa ttccctcatc
      661 attgataagg ttggacatct ctttcaacat gagagtcatt gaaccattcg accaatttag
      721 cggaatgata gggcttggcg cttcatgcaa agttcccgac ttgtggatat agttatagct
      781 ttcgggaagt gccatttcag gtacactcct gccatttgca ccaatcatat tctcgcctgt
      841 tatttgggcc aatgatctat tcataaattg cactgagtca ttcaaatact cttcctttct
      901 tgattcttta taaacaattg ctgctgattt tgcataccat gagtcaaaaa accattctgc
      961 ttctgttgat ggaatgaagc tcttttctct tttcgcatgt gaattttgat cggtatcggt
     1021 tttaatatca ttgaaccaaa aatttgccga ctgataatta tctttctcgt atctttttat
     1081 accatacgga cctgctagtt ttttaactat ttttagaacc tgctcttttc ttctggtgtt
     1141 tatctttgca agatttgcag gataaataac attaagtaaa gccgcatcgg cttctctgta
     1201 atgtttatct ttttccagat aacctgggga ttctccccct aaatcaagct gtaacgttat
     1261 tctttcataa cctttatcta tgaggtggtt caacctcgtt gttgaaagag gttcatccag
     1321 ttcattggct ttagcttcgc gtagtaaatc agagacaaaa acagagtctt tcttcgagag
     1381 taagttcgac aaacgttcca gtccagaggt caccagtgca acggaagatg tgtttaatcg
     1441 ggcatcttct tcccatgctc cggaatcttc cataacatag aaattggctt tatccagata
     1501 agcaatcagc agcgcaaccg attttaatcg gtcacctttt tgccaatctt cagcgttaat
     1561 tgtatcagtg tcgattgctt gtataaggag atcaagataa agacctaacg catcattttg
     1621 tttatggttc cacagctgcg gcttgccttc ttcctgcaca tctgccatca cgggggagtt
     1681 gctgtcaaat cggatatgca caacattcat ttgcccggga actccatcca gtcgtttggg
     1741 gttgctaatg acgtcctgca tccgcttaat ctggtcgggc gtagacatat aatcccacag
     1801 ggtgagcaga accttttttg cggctacact atttccctga tctgatacta atgccatata
     1861 gccccataaa ctatccctca gccagacagc atcatagtgg gtttctgccg ctgttttgtc
     1921 atcttcgaaa gtagaagcaa gaataaacgt tccccagggt ttctccgtca acgttaactt
     1981 tcgtgtgttt tctgttaatg ttagcaatcc ttgaatttcg tctttggtat agtggctctt
     2041 tatttcattt tgtaatttgg ctgagtgaat caaaagacga taattaggat acttatctgg
     2101 atttaacgat aaataacttt tatctgttgc tgcccccgtg gaattaagaa tgttaaatac
     2161 agattcactc gtcacttctg cccctgtcgc ctggcatgtg ccgcacgaaa atataccagc
     2221 gagcagaaga gttaatgcag atttcccttt cataaaatat tccatcaatc gtttgttaaa
     2281 agcgtagata accacttatt taacaacttg aaaataaggt gttttacctg ggttgttaca
     2341 aaaggattgc attgcgtaaa cgctttttat ttacaacaaa atggggaagt attacggcga
     2401 ttgcgaaaag tctgaagcga ttgaattaag ataatataga aaatcagata cagcaaaaat
     2461 ggcagattga tcaggtgaag ataaattgga gcgggcgaag ggagcctacg acaaatcgaa
     2521 gacaaccagc taacctcttg tttcttaaag actgatgaat ccagttggtt gtagtgatag
     2581 tcacacattt agcgatagac ggtcaagact tgaagcacgt aatattccct ctaaaggaat
     2641 agaactcacg gtctgactac ccggcccttt tggtctgact cagggtcggg ctatggggac
     2701 ttttggctcg ctctttatat tagatgaggt ctcacatttt tcagtcaaaa ctcatcgtct
     2761 aatagcccat agccagacca ccagtcccag ctttagcagg aagaccacga gatagacttt
     2821 gtgtaggtcc atttggtcct cctcccttct gtattcacga actagattac tagtacaaaa
     2881 taaaggccag acatccagtt cggaagtctg accaatagtc gttattgtta gttagcttct
     2941 ttagtgaagc tcaggcgttt gactacaggc aggacagcag tagccgagcc agtccccatc
     3001 ttgatggtcg tagcagcttc agctacaaca aacaccttgc cgagaatctc aaggtcgtga
     3061 gactgggagt caccatcacg tgaaaggacc acagtaatgt ggtccccatc gtgacggaag
     3121 caaccgaagc tcagagccgt aaaacgctta gccatcatga agacgccacg gtcttcaccg
     3181 ttgtaatagg cgatgtcagt ccccaacata tcgacgatag cgtcttcacc accaaagccc
     3241 tgcttagcag caagacgtga cagtgccttg aggtgtgact catgggcgat gatgccgtaa
     3301 tcagcaacct cagaaccgaa agaaggctcg gtgttctcgt acagcgcgtc catgatagtg
     3361 tctgccttag ctgtcaggct accagtcagg accggaagct ctacagtctt caacagcttg
     3421 gaggattcca gtacatcgac catgcgacta ttgatggtac ggaccacgtt atcgcagatg
     3481 ttcagagcgt cctgctggag cgtcccctga gtgaaaccag tggaagactt agcgtcccat
     3541 agcttggtct ggtgggacac gaggatagct tgctcaatcg gctcaacggt gatttcagta
     3601 ccagacggag cggtcacgac ttcaggtcgc ttaccaccac ggacaggaac gaccacagag
     3661 cggtcagcca gagtgtgagc aatgattggg gtcggtaagc tattgcgagt tggcagaatc
     3721 tcaatcggga atggaatgtt atcctgagcg gacttcagca caccattcag catggtcttg
     3781 acagctttag ccagtgccag agcatagacc tgtgggtcag cattggttgg cagaccttcg
     3841 ttaacctgaa tgttgtctgt gtcggtgata cgtactggct caatgccgaa ctgatagctg
     3901 tctttagtca tagtcataat aatcaatctc ctaaattagt ttagatacgt gggtcccgac
     3961 atcaggtcag gaccacttgt gtgcgtctgt cttacaggtt gtccatccag ttaccgcctg
     4021 actcaaggtc agcgacgata gcggcacggg agcgaccagc ggaatgggta gcactacgaa
     4081 ggttctcacc gtaggtccgg cgttcagctt tgacctgttc acggcgctgt agctctttct
     4141 gccactctgc atcagtcatg ttctcttcac gcatgcgctc ggcctgaatg cgctgctctt
     4201 tagcgatacg ctcagcctca gctcgcttgt tctgctggcg gcgaccatag gcggtcatca
     4261 ggtcattact gaagaagtta ccaatggtca cgccagcttc agccagggct tccaataact
     4321 ccatagactc gcggctggta gacggcaggc ctgacaggta gcccggaatc agggtcttga
     4381 tagcgaggga cagcggatag aactgaccgt ccagactcac agtattctcg tagagattga
     4441 tgtcggtgac tgacagtttc cagtcttggt tattgaataa cgcactagct ttgct
//
LOCUS       DAZGLJ010000297         4445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000297 DAZGLJ010000000
VERSION     DAZGLJ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.297"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            163..1563
                     /gene="gabP"
                     /locus_tag="ACHK49_004608"
     CDS             163..1563
                     /gene="gabP"
                     /locus_tag="ACHK49_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO9265098.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPALMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            1584..2246
                     /gene="csiR"
                     /locus_tag="ACHK49_004609"
     CDS             1584..2246
                     /gene="csiR"
                     /locus_tag="ACHK49_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO9265099.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(2247..2696)
                     /gene="kbp"
                     /locus_tag="ACHK49_004610"
     CDS             complement(2247..2696)
                     /gene="kbp"
                     /locus_tag="ACHK49_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO9265100.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(2780..2938)
                     /locus_tag="ACHK49_004611"
     CDS             complement(2780..2938)
                     /locus_tag="ACHK49_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO9265101.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            3121..3420
                     /locus_tag="ACHK49_004612"
     CDS             3121..3420
                     /locus_tag="ACHK49_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO9265102.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            3430..3954
                     /gene="ygaP"
                     /locus_tag="ACHK49_004613"
     CDS             3430..3954
                     /gene="ygaP"
                     /locus_tag="ACHK49_004613"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO9265103.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRT"
     gene            complement(4001..4405)
                     /gene="stpA"
                     /locus_tag="ACHK49_004614"
     CDS             complement(4001..4405)
                     /gene="stpA"
                     /locus_tag="ACHK49_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO9265104.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         1034 a         1090 c         1126 g         1195 t
ORIGIN      
        1 acgcggctac accctcaccc cggccctctc cctaagggag agggagaaaa ctctcccgct
       61 ttcgttttta agattttgtt gaccgtctca attaaacgcg tgacccgacg cgggaaatgt
      121 cggggcgctc tcccaagtga cacactttcg agaggattca ggatggggca atcatcgcaa
      181 ccacatgagt taggcggcgg gctgaagtca cgccacgtca ccatgttgtc tattgccggt
      241 gttatcggcg caagtctgtt tgtcggttcc agcgtcgcca tcgccgaagc gggcccggcg
      301 gtattactgg cctatctgtt cgccggatta ctggtggtta tgattatgcg gatgttggcg
      361 gaaatggcag ttgccacgcc cgataccggt tcgttttcca cctatgccga taaagccatt
      421 ggccgctggg cgggttatac catcggctgg ctgtactggt ggttttgggt actggttatc
      481 ccgctggaag ccaacatcgc cgctatgatc ctgcactcgt gggttccagg cattcccatc
      541 tggttatttt ccctcgtcat taccctcgcc ttaactggca gtaatttatt aagcgttaaa
      601 aactacggcg aatttgagtt ctggctggcg ctgtgcaaag tcatcgctat cctggccttt
      661 attttccttg gtgcagtcgc aattagcggt ttttaccctt atgccgaagt gagcgggatc
      721 tcaagattgt gggatagcgg cggctttatg cccaacggct tcggtgcggt attaagcgcg
      781 atgttgatca ccatgttctc gtttatgggc gcagaaattg tcaccattgc cgccgcggaa
      841 tccgacacgc cggaaaaaca tattgtccgc gctaccaact cggttatctg gcgtatttct
      901 atcttctatt tgtgctctat ttttgtcgta gtggcgttaa taccgtggaa tatgccgggg
      961 ctgaaagccg ttggttctta tcgctcggtt ctggaattgc tcaatattcc ccatgcgaaa
     1021 ttaatcatgg actgcgtgat attactttcc gtaaccagct gtctgaactc ggcgctgtat
     1081 accgcgtcaa ggatgctcta ctccttaagc cgtcgcggtg atgcgcccgc gctaatgggc
     1141 aaaatcaacc gcagtaaaac cccgtatgtg gcggtgttac tctccaccgg agcggcattt
     1201 ttaacggtgg tggtgaacta ttacgcacct gcgaaagtgt ttaaattcct gatagacagc
     1261 tccggcgcta tcgccctgct ggtttattta gtcatcgccg tttcacagtt gcggatgcgc
     1321 aaaattctgc gagcagaagg aagcgaaatt cgcttgcgca tgtggcttta tccctggctc
     1381 acctggctgg tcatcggctt tattaccttt gtgttggtag tgatgctatt ccgtccggcg
     1441 caacagttag aagtgatctc caccggctta ttagcgatag ggattatctg taccgtgcca
     1501 attatggctc gctggaaaaa gctggtattg tggcaaaaaa cacccgttca taatacgcgc
     1561 tgataatgat caggagtcac accatgacca ttacgtctct ggatggctat cgctggctga
     1621 agaacgatat tattcgcggt aattttcaac cggatgaaaa attacggatg agtttgctga
     1681 catcgcgtta tgcacttggc gttgggccgt tgcgggaagc actttctcaa ctggtggcgg
     1741 aacggctggt cacggtggtg aatcaaaaag ggtatcgggt ggcgtctatg tcagagcagg
     1801 agctgctcga tattttcgac gcccgcgcca atatggaagc gatgctggtc agtctggcga
     1861 ttgcccgcgg tggcgatgaa tgggaggcag acgttctcgc aaaagcgcat ctgctcagta
     1921 agctggaagc ctgtgacgcc agcgagaaaa tgcttgatga gtgggatctg cgtcatcagg
     1981 cgtttcatac agcaattgtg gcgggctgtg gttctcacta tttgctgcaa atgcgtgaac
     2041 ggttgtttga tctggcggcg cgctatcgat ttatctggct gcggcgaacg gtgctttcgg
     2101 tagaaatgct ggaggataaa cacgatcagc accagaccct gactgcggcg gtactggcgc
     2161 gagataccgc gcgcgccagt gagttaatgc gccagcattt actgacgcca attcccatta
     2221 tccagcaggc gatggctggc aattaactac tcttccggaa tacgcaacac ttgccccgga
     2281 taaattttat ccgggctttt tagcatcggt ttattcgctt cgaagatttt attgtacaga
     2341 ttagcgttac cgtagacctg tttggaaatg gcactcagag tgtcgccaga cttaacggta
     2401 taaaactggc tggcagtggc tggtgtcgcc gttttcacct gatcatcgac actggcaata
     2461 ccggaaatat tcccaaccgc aacaaggatt ttctccttcg cctcctgact caggccgtca
     2521 ccagtgaccg tcgctttgcc gtcggcaatt tgaatgttca ctttatcggc atcaggtata
     2581 ccggttttgc tcagatgctc ctgcaccttc ttcgcctgat cgtctttatc gtgctgacct
     2641 gtaaccgcgt cccagagttt ttctccggca tctttcacaa aattgaacag acccatagct
     2701 acctcattcg ttaacggaaa cactcagaaa gtgtagcaga ggaacgcggc gcgttaacgc
     2761 cgcgggggtg agcacaggtt taatcgcggg tttgcaccca gaacgcgtga atcaaaccgg
     2821 ggatataccc aagcagtgtc agcagaatat taataatgaa cgcccaaccg aaccctttac
     2881 cgagcagcac gccgagcggc ggcagaatga tggtgatgac gattctccag aaacccatat
     2941 gtactcccta taagaaaatt actcattgtt taaaaagaga ttttatctct taagcgtagt
     3001 aattttagcg gagcctgcca gtttcagctt acgcttttac tctcatttac gctgctgagg
     3061 ctggtatatg ttgcattttt agtttagagt ttacttaatt tagaaaatac ttaaataaat
     3121 atgactgaac tcgcgcaatt acaggccagt gccgaacagg cagcggcctt attgaaagca
     3181 atgagccatc ctaaacgatt gctgattctg tgcatgctta gcggttcccc cggcaccagc
     3241 gcgggagagc tgacgcgcat taccggactg agtgcctctg cgacatcaca gcatctcgct
     3301 cgtatgcggg acgaagggct tatcgacagc caacgggatg cccaacgcat tctatattcc
     3361 attaaaaatg aggcggtaaa tgccattatc gccaccctga aaaatgtcta ttgtccgtaa
     3421 ggagtcacta tggctttgac aaccatttcg ccgcatgatg cacaagaatt aatcgcacgc
     3481 ggcgcaaagt taatcgatat tcgtgacgct gatgaatatc ttcgtgaaca tattcctgaa
     3541 gcagatctgg ctccattatc cgtgctggaa cagtcaggtc ttccacctaa attacgtcgc
     3601 gagcaaatta tattccactg ccaggcaggt aaacgcacca gtaataacgc cgataaatta
     3661 gcagcgattg ccgcccccgc agaaatattt ttactcgaag atgggattga tggctggaaa
     3721 agagctggat tgccagtagc ggtaaataaa tctcaaccct tgccgttaat gcgccaggtg
     3781 cagatcgctg cgggtggttt aatattaatc ggcgttgtac tgggttatac cgtaaatagc
     3841 ggtttcttct tattaagtgg ctttgttggt gccgggttac tgtttgcagg aatcagcggt
     3901 ttttgtggaa tggcaaggtt gttagataag atgccgtgga accaacgaac ttgagaagcg
     3961 acgccggacg cgccctagca gcgacatccg gcctcagtaa ttagatcagg aaatcgtcga
     4021 gagatttacc ttctgccagc gcctgagcaa ttggcttcgg tgtacggccc tgaccggtcc
     4081 aggttttagt ttcaccgtta acatcggtga atttatattt cgccggacgc ggctggcgtt
     4141 ttttaccagc gcgtggtgca gcagcagagc tattacctaa taactcttcc gggttaattc
     4201 cgtcagcctt catcagctcc agccaggtgc taattttttc ctgacgctca gccagttcac
     4261 gctgctgctg ttcttcttct tcacgtcttt ctttagtgac aaccctgaat ttttcgagca
     4321 tttcttcaag aacgtcaatg gagaattcgc gagccatcgc acggagagtg cgaatgttat
     4381 ttaaactttg taacattacg gacataataa agaaaacctt ttaacgccaa aacaaaaaag
     4441 tattt
//
LOCUS       DAZGLJ010000298         4409 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000298 DAZGLJ010000000
VERSION     DAZGLJ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.298"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..311
                     /locus_tag="ACHK49_004615"
     CDS             <1..311
                     /locus_tag="ACHK49_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO9265105.1"
                     /translation="TIQTLKQTRAMTGEILETWEDGHTLWASVNMISSKEAISSGAEL
                     AIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRLEILCREGAEK
                     "
     gene            308..604
                     /locus_tag="ACHK49_004616"
     CDS             308..604
                     /locus_tag="ACHK49_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO9265106.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLEFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            604..1044
                     /locus_tag="ACHK49_004617"
     CDS             604..1044
                     /locus_tag="ACHK49_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO9265107.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            1028..1210
                     /locus_tag="ACHK49_004618"
     CDS             1028..1210
                     /locus_tag="ACHK49_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265108.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            1333..1689
                     /locus_tag="ACHK49_004619"
     CDS             1333..1689
                     /locus_tag="ACHK49_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO9265109.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            1673..3334
                     /locus_tag="ACHK49_004620"
     CDS             1673..3334
                     /locus_tag="ACHK49_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO9265110.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLESPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVMHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSTCKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGATPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLVSRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            3348..3629
                     /locus_tag="ACHK49_004621"
     CDS             3348..3629
                     /locus_tag="ACHK49_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265111.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
BASE COUNT         1164 a         1060 c         1238 g          947 t
ORIGIN      
        1 taacgattca gaccctgaaa cagaccagag ccatgacggg cgaaatactc gaaacgtggg
       61 aggacggtca cacactctgg gcaagcgtga acatgatcag cagcaaggag gccatttcat
      121 cgggcgcaga gctggcgatt ggtaccgtaa ggatctggat acggtaccgg aaggacatca
      181 acgccaccag tcggataaag gtcagtacgg ggccgctggc ggggcgtgta ctgaatatca
      241 tcgggcagcc gctgccggat gccgccagga cacgccttga aattctttgt cgtgagggcg
      301 cggaaaaatg acagaagaac ttatcaccct ggaagaagtg aaactccatt gccgcatcga
      361 tggcgacgat gaagaccagt taatcagcgg atacattgcc gcatcgcttg aggcgtgcca
      421 gatacacata ggcaggcgct ttgatgacgg gctggagttc acgccagcca taaagattgg
      481 ctgcatgatg tttatcgctc acctgtatga aaatcgccag attgtcgcgg ataacgcaaa
      541 aacacgcgta cccatgacga taggcgcact ctggacggct taccgtgatg tgggggtgta
      601 ctgatgccgt ggcaaccatt aagacgatgc acagaaccag gctgtaacag gcgcgttaaa
      661 tccggcaagt gtgaggagca caggcgggcg gcatggcgtg cagaggatgc cagacgagga
      721 caccgccgcg cgcgtggata ttccagacag tgggacaaat accgcgccat gtatctgagt
      781 aaaaacccgt tatgcgtgcg atgccttgag aaggggatat atacgcccgc cgtggtggtg
      841 gatcacatta tcccgattaa tggcggtgat gatgttctct tctggcccga atggaaccat
      901 caaccattgt gccaggcgtg tcataaccag aaaacgaaat ggcttgatcc ggcaacaaaa
      961 agcaagcgtg ccgcaggtgg atttcgtgaa gaggaagaac gggccgctaa ccgcaataac
     1021 tggatgtatg acgctgatga atgagcggga gcaaaaccgc cttatccgtg gacttataag
     1081 gcagcgtgac gcatggaaga cacaggaaac agggcataaa gataaagcgt caggacgcgc
     1141 agaacgcatc acagcgaagc gattaaccga ccgtgaccgc gaggttatgg aatgtttccg
     1201 caatcgctga tgaggctgta tgacggggtg gggggcgttt tcaggaccaa cccgaccccg
     1261 ccgggcaccg aacgcctcct caaattttta tgcacgggaa ttttttgaaa aataatctgg
     1321 cgaaaaataa gcatggcaag accaccgaaa gcccccgcct acctggatga tatcgccgtg
     1381 aagcagtggc gggaaaaatc taggcagctt gcggaacggg gagacctgac ccccgccgac
     1441 tggagcaatc tggaactgta ttgtgtcaac tactccattt accggaaagc cgttgcagac
     1501 cttgcggcgc gcgggttcag cattgttaac agtcagggcg gcgagagcag aaaccccgca
     1561 ttaagcgcaa aatccgacgc tgaaagagtg atgataaaaa tggcctcttt gctcggtttt
     1621 gacccgataa gccgccgtaa aaatccaccg gaaacagaag aagaggacga gcttgaccgc
     1681 ctggaataag tacgcagaag acgtaaaaac gggcaaaatt ccggcctgta aacggctgaa
     1741 acaggccgtt aaacggtact tttcggacct tgaaagcccc ctttacacgt tcgatcgtga
     1801 ggttgtggag cggtttattg ccttttccag ggtgtgcccg cacgtaaaag ggccgatgcg
     1861 tggcagaccc attgagctgg agccgtggca gcagttcgcc tttgcgtgca tcctcggctt
     1921 taaggttaag gccaccggac ggcgcaaata cacgagcgcc tttatcgaag tgccgcgcaa
     1981 aaatgcgaaa tccacgaccg ccgcgattct ggctaactgg tttctgatta tggagaacgg
     2041 gcagcaggat atttacaccg cagcggtgag ccgtgaccag gcgcggatcg tttttgatga
     2101 tgcgcgtcag atgtgccttt tatcccgacc gttacgcagg cgggtgaata ttcaggccca
     2161 caaggtgatg cacccgaaaa gcaacagcct gttaaagccg ctggcagcaa aagcggcaac
     2221 catcgaggga acaaacccca gcctttccat cgtggacgaa taccacctac accccgataa
     2281 cggcgtttat tccgcgcttg agctggggat gggcgcacgt cccgaagggc tgttatttgc
     2341 cattaccaca tcgggcagta acgtcgtttc aacctgtaaa cagcactatg attattgctg
     2401 ccagatcctg gacggcgaag aggtcaacga ttcaattttt gtgctgattt acgagctgga
     2461 cgacgaaaac gaggttgatg atccggcgat gtggataaag gctaacccta acatcgatgt
     2521 ttccgtggat cgtgaaaaac tggcctcaac catccagaaa gcgcggggta ttccgtcgca
     2581 atgggtggag atgatgacaa agcgatttaa tatctggtgc cagggggcta ccccgtggat
     2641 gggtaacggc gcatgggctg agtgtgccgg aacgttcacg gaggaagatt tacacgggca
     2701 ggagtgttac gcggggctgg acttatcatc aaccagcgat atttccagcg tgtgctatgc
     2761 ctttccggtc ggtaaaacca ttatgctggt ttcccgtcat tatctgccgg agttccagct
     2821 acagaacccc gccaataaaa accgcgctgt ctatcgccag tgggctaaag cgggctggat
     2881 acgcacaacg ccaggtgact gcattgatta cgaccggatc agagatgaca tcatgcagga
     2941 tgcggagaaa tttaatatca ggctggtggg ctttgatacg tggaacgcca cgcatctcag
     3001 gacgcaatta cagggggctg gttttgaggt ggagccgttc ccgcaaacct acctcagatt
     3061 cagtccggcg gcgaaatcgt tcgaagtttt tgttaaccgc agggtgattg tgcatcgtgg
     3121 cgatccggtg ttgtcctggt cgatgagtaa cgtcgtgatg cagagtgacg cgaacgccaa
     3181 tatcaagccg aacaagaaaa aatcaccgaa caagatagac ccgagcgtag cggcgctgat
     3241 ggcgtttggt acattccagg cagagcatga ggattttgct tttgatataa gcgacagcca
     3301 ccgccagaaa ctggaagaat ttagcggggt gtaatgaggt cagcagcatg acagaagccg
     3361 aaatactgcg attaatccgc cgcgtctctg gaatcagcca gcaggctgac gaacagacca
     3421 cgcagccgga cagcgtgaca gccgaaaatt atgcgcgtgt tgttgctgag gtgatgcgcc
     3481 gtgatggtat ccagcttaat gatgcggata tgcgcgacat acggatccgc gttctggaaa
     3541 tgctggccta caatcgccgc gttgaactgt atcgggagaa ggagaaaatc acgtatcact
     3601 ggaagaagcc ggagcggtta cggcggtaac gtgttgattt aaaggcggct caattttgag
     3661 cctcgtaact ggttgatatt tccggcaacc tcaatttgag gaagtcggcg ctgtaactcg
     3721 ctgaacttaa agcaaagctc aaatttgcgc tggctgaatg atcattatga ttacttatgt
     3781 ttacgcagat gattaaggcc taaaccgttg attattccac aatcccccat tgggggaagc
     3841 tggcggtaac tggttgaact taaagcaaag tgcaaaattg cgctttgcta acccgttgat
     3901 attctcggaa acagccaacg gttgaggccg gaacaaccga cgatctttca gatagtcaca
     3961 agctacgaaa ttttcgcagt ttaattactc gccagtttat cgttaagtag ttgatatttc
     4021 cggtaacctc aatttgagga gagcggaaac atcatccctc acattctgaa tggtcataat
     4081 gaccacgcag gccatcgagg caaccatagc gatcatcgtt acggttggag ctttccgcca
     4141 ttggtggaaa aggtgcacat ctggatcatc attacggtga tcaagggtat gtaaaaccca
     4201 ccagcctgat taaatgttaa ccggaaaaaa aatccggtag ccccgttatg gggatcccca
     4261 tatcgacatt aacgcccctc atgaattggt gcgcttcccc ctggaaagat tacccgcctg
     4321 tatatttctt gtgtctattt gttccacgtt gtttcataca gtgcaccgaa cggtgtagtt
     4381 aatggtgtag tcaatttgcg atttttggc
//
LOCUS       DAZGLJ010000299         4397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000299 DAZGLJ010000000
VERSION     DAZGLJ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.300"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            498..1247
                     /locus_tag="ACHK49_004622"
     CDS             498..1247
                     /locus_tag="ACHK49_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HFO9265112.1"
                     /translation="MSKKYQLLLITHYMSTWVTITEAVEITTKAIKQKITPSDIYRHA
                     LSGNILLLVYFQSPVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCT
                     EARYICPVQQIIDTPLLRKLNQFRTFVRNVRPGDELDVQAQVSEKNLTPPPGNSSGNL
                     EQQIASTSQLIGSLLAEDMNSEQAANIARGWASSQTSGVMTDWLSRFGTARITLGVDE
                     DFSLKNSRDGNPRKWRFDATNSELCAERHEC"
     gene            1174..3296
                     /locus_tag="ACHK49_004623"
                     /pseudo
     CDS             1174..3296
                     /locus_tag="ACHK49_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(3466..>4397)
                     /locus_tag="ACHK49_004624"
     CDS             complement(3466..>4397)
                     /locus_tag="ACHK49_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO9265113.1"
                     /translation="MAIFPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVT
                     MTMQDHATLKEIKDLAKNITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGL
                     TMKCYSPLCKNASRMDQNLRTPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEVREE
                     MRSLGSYIALNLDGSSQERTFSLSIAENLIAKIQSETDIPIVIVYGPKGEDKARALVD
                     CYNNVYRLSLSPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPA
                     MADVSESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT         1345 a          998 c          927 g         1127 t
ORIGIN      
        1 ctctccttat actaaagaaa taatcatatc aaaataaaaa ttcacaacag tgcaacatta
       61 aaaaatacaa ccaacaaaca atcctatata caaggcacat ctccagaata taaaagcaca
      121 gacaaacaac ctaaaaaaca accccataca tataaattat tttataggta aaatagatta
      181 tatattctca tagctaccat aaactatact agcctgaact atatttattc tgctgcaatc
      241 aatgcataca taacacaaat atcactcagg tacactactc aaaccacgct gggatttttc
      301 ctcaagttat aatcatcacc ccgaaaatca ttcggcattt actcattaaa tagtcacccc
      361 ataggcctgt acatgtttac tcagaaatat acatcctttt ctctgtcata aaccctctga
      421 ttaatcataa ataaatactt gtgacaccaa tctttttcct taacggaacg aattgttgtg
      481 tagaaggaga taatattatg tcaaaaaaat atcagctatt gcttataact cattatatgt
      541 caacatgggt cactataacg gaagcagttg agatcactac caaagccata aaacaaaaaa
      601 ttactcctag cgatatttat cgtcatgcct tgagtggcaa catcctacta ttggtctatt
      661 tccagtctcc tgtgatactt aagaagatac aaacttttaa tggaaaaata aaattcaggc
      721 agtttgtggg agacctactt gataaactat gcatgcttga cagggacggt tttatttatg
      781 gtcaaaattt aagactctgt acagaagcca gatatatttg tcctgtgcaa caaattatag
      841 atacccccct gttacgcaaa ctcaaccagt ttcgtacgtt tgttcgtaat gtccgcccgg
      901 gtgatgaact ggatgtccag gcacaagtta gtgaaaaaaa tttaaccccg ccgccgggta
      961 acagcagtgg caaccttgag caacagatag ccagtacttc acagctaatt ggatctctgc
     1021 tcgccgaaga tatgaacagc gagcaagcgg caaacatagc acgtggatgg gcctcttctc
     1081 agacttcagg ggtaatgaca gactggttaa gccgcttcgg taccgcaaga atcacgctgg
     1141 gcgtggatga agatttcagc ctgaagaact cccgtgacgg caaccctcga aaatggcgat
     1201 tcgatgcaac aaacagtgaa ctatgtgccg aacgtcacga atgctgaaat cacgctggca
     1261 gccttgaagg atccggtgat tgccgacaat aacgatctca cgacactaac agcaccgtcg
     1321 ctgatacaga gggcaatgcg atagccaaca ctgaggtaac atttactcta ccggaagatg
     1381 tgaaggcgaa cttcacgctg agcgatggcg acaaagcggt tactgatgcc gatggcaaag
     1441 cgaaagtcac gctgaaaggt acaaaagcag gcgctcatac cgtgacagca tcgatggttg
     1501 gcggtaagag tgagcagtta gtggtgaact ttactgcgga tacgctcact gcgcaggtta
     1561 atcttaacgt taccgaggac aatttcatcg ccaataacat tggaatgacc aggctacaag
     1621 caatagtgac tgatggaaac ggcaacccag tagaaggtat aaaagttaat ttccgtggaa
     1681 cctccgtcac gctaagcagc accagcgttg aaacggatga tcaggttttt gctgaaattc
     1741 ttgtgacaag caccgaggtc ggactaaaaa cagtttcagc ctctctggca gataaaccta
     1801 ctgaagtcat ctcgcgatta ctgaatgcca aggtagatgt taattctgcg acgattacca
     1861 gtcaggagat acctgaaggt caggtcatgg tcgcacaaga catagcagtt aaagctcacg
     1921 ttaacgacca gtttggcaac ccggtgacgc atcaagccgc gacattcagt gcagcgccat
     1981 cctcgcaaat gatcatcagc cagaacacgg tctctactaa tacgcaggga gtagccgagg
     2041 tcaccatgac gtctgaaaga aacggctcgt atactgtaaa agcatcccta gcgaatggag
     2101 cttcacttga gaaacaactg gaggctattg atgaaaaact gacactcacg tccagtccgc
     2161 ttatcggtgt caatgctcct aaaggcgcta ctctgacggc aacgctaacc tctgcaaatg
     2221 gcactccagt ggagggtcag gtcatcaact ttagcgtaac gctagaaggt gcgacgttaa
     2281 gtggcggaaa agtgagaacc aactcttcag gtcaggctcc agtcgttctg accagcaata
     2341 aagtcggtac atatacggtg actgcatcgt tccataacgg cgtcacaata cagacacaga
     2401 caaccgtgaa ggttactggc aacccaagca ccgcacatgt tgctagcttt atcgctgatc
     2461 cgtcgactat cgccgccacc aacagtgatt taagtacctt aaaggcaacg gttgaggatg
     2521 gcagtggtaa cctgatcgaa ggtctcactg tgtacttcgc cttaaaaagc ggctccacca
     2581 cattaacgtc attaacagcg gtgaccgatc aaaacggaat cgcgacaaca aacgtgagat
     2641 gagcgataac agggagcgtc actgtaagcg cagtcataag cgcaggtgga atgcaaacag
     2701 tagatatatc gctagtggca ggcccagcag acgcctcgca gtccatcctt aagaacaacc
     2761 agtcatcact gaaaggggac tttaccgata gtgctgagct acatcttgtt ctgcacgata
     2821 tatcaggcaa tccgatcaaa gtttctgaag ggatggaatt tgtgcaatca ggtacaaacg
     2881 tgccctatat gaaaattagc gcaattgatt acagtcaaaa tatcaatggt gattacaaag
     2941 ctactgttac aggagacgga gagggtatcg caacgctgat ccccgtattg aatggtgttc
     3001 atcaggcggg cctgagtacc acgatagaat tcattagcgc ggaaaccaga ccaatgacgg
     3061 gtacagtgtc agtgaatagt gcaaacctac cgacagcttc attcccttcg cagggattta
     3121 ccggggcgta ttatcagttg aataatgaca actttgcacc aggaaaaacg gcggctgatt
     3181 attcgttttc aagctcggcc tcctgggtag gcgttgatgc tactggtaaa gtgacattca
     3241 aaaatgacgg tgatagcaat acagtaataa tcacagcgcc cccaagatcc ggtggtgcta
     3301 tttaccaaac agttcctcct gaatcgcgtt cggtgtgact atgatcattt tcggtgagta
     3361 ttgctttgat ccaaaggctg gttttctgtg atgattgacc agcctcagag tattgatgac
     3421 accaccgttc ataacagatc ggcattacta tgcataagta ctgcctcaaa ttctcgccaa
     3481 aacacttttt aatgcctttg cgaattcatc cagacttata ttgtcaattt tttgcccaac
     3541 gacgactgac tccgaaacat cggccattgc gggccatcgc gttttgtaat cagcataaat
     3601 tgcaacaaca ggggtattat aggcacttgc catatgtaat attgaggtgt caggagttat
     3661 tgcaatataa gcatctttta tgattgctgc tgaacgttta atcgagggtg ataaggataa
     3721 acggtagaca ttattataac aatccactaa tgccctggct ttatcttccc ctttgggtcc
     3781 ataaacgatc actattggaa tatctgtttc actttgaatt tttgctatta gattttctgc
     3841 aatcgataat gaaaatgtac gttcctgcga gctaccgtct aaattgagcg caatgtaaga
     3901 tcctaacgat cgcatttcct cgcgtacctc atcgagtaca tcatcgctta atggcaattc
     3961 atatattgac ctgactgccg gaaaacccgc ctcacgcatc ataaatgccc atgtcatggg
     4021 tacaggagtc cggagattct gatccatccg ggacgcgttc ttacacaaag gggaatagca
     4081 cttcatggtt aaaccgacga cctggaaatt cgtttttgct ttcaactgac tgataaatat
     4141 catcgttttc aggttctttt tacgcatcgc ctctatacaa agatccggcg taccgtattg
     4201 cagtgttata ttcttcgcta aatcttttat ttcttttaat gttgcgtgat cctgcatagt
     4261 cattgtgact aatgttaatt tagtctgttc aagtttgagc gcattaaaga cttctaaatt
     4321 aattgtcgac gtaacaatta atagatgctt aattttatgc aattcaagcg cccgaataac
     4381 aggaaagatg gccatag
//
LOCUS       DAZGLJ010000300         4393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000300 DAZGLJ010000000
VERSION     DAZGLJ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.301"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            518..778
                     /locus_tag="ACHK49_004625"
     CDS             518..778
                     /locus_tag="ACHK49_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9265114.1"
                     /translation="MNSISVSTPAPAAPVLPVPAQQERFIRLPEVMHLCGLSRSTIYD
                     LISRDAFPQQISLGGKNVAWVQSEVSAWMAARIAAREQGGHA"
     gene            775..1323
                     /locus_tag="ACHK49_004626"
     CDS             775..1323
                     /locus_tag="ACHK49_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO9265115.1"
                     /translation="MKLALQSHLLFSGLRPDIFSRYSFPAAAKSAAGRRNPSYLLATP
                     DAPCVFFYVVAQAHPFFGLRCLYLSPFQIMVVRAGQPSGWPVSNKAGIPTPVRVTTHE
                     RRNSGGGIYRYLLEVALMATILAPSHPQFIFVFAAVRRAERKPRISMLRIIASNEHHA
                     RLSLVHEYVLCLAARLPVREVI"
     gene            1323..1547
                     /locus_tag="ACHK49_004627"
     CDS             1323..1547
                     /locus_tag="ACHK49_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265116.1"
                     /translation="MNHAAISYDDILRLKHLRNVGEFVTGMAVLQDCYEKPASAQCEQ
                     LVSLIYLMTEQLDGVVQRCQDDLMNMEVVQ"
     gene            1544..1867
                     /locus_tag="ACHK49_004628"
     CDS             1544..1867
                     /locus_tag="ACHK49_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HFO9265117.1"
                     /translation="MKSRTLSLSLRVALYRRAVACAWLSACYQQNRHLQLTLDEIETA
                     IARELEGFYLRQHGQTKGMEIACALLSDLMEYGPLMSCPALSQLGIAVMDELCVRHIK
                     KPVLH"
     gene            1882..4215
                     /locus_tag="ACHK49_004629"
     CDS             1882..4215
                     /locus_tag="ACHK49_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO9265118.1"
                     /translation="MSGMKVSQAVKAARGHWAQILPALGVNILKNRHQPCPVCGGKDR
                     FRFDDQEGRGTWFCNQCGAGDGLALVTRALNVDISEAADRIHGLTHDLSVVNSEVRTL
                     TADTDSGKDAAAALAARLLQASRESAGNTYLTHKGFPEHICHELTSAHKAGGMMFRPG
                     DLIVPLYNADGELVNIQLISGNGSKCFLKGGQVKEAYHLIEGGGSSVRRIWIAEGYAT
                     ALTIHYLTGEAVMVAFSSVNFLSLSSVAHNKYPGYQLIIAADRDLNGSGQNRAETAAK
                     ASQCDIVLPPVFGDWNDALTQYGEESTRKAILEALKPHNASPFDTMSEAEFTAMSVSE
                     KAQRVREHYRDALAVDPNGQLLSRYESGAWKVISQSDFARDVAALFQRLGAPFSSGKI
                     ASLVETLKLIVPQQQNPSRHLIGFRNGVLDTRTGLFSPHCKENWLRTLCEVDFTPPVK
                     GETLETHAPAFWRWLDRAAGHKPAKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIETLESSRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDSKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPEQIAPEERD
                     PQLKDKIARELAVIVRQLMQRFSDPMSARTLLQSQQNSDEALTIKRDADPAFDFCGYL
                     EALPDTNGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPVMLKEY
                     GLHYEKRRTNQGMQTNLTLKEESNADWLPKCDEPILK"
BASE COUNT         1052 a         1029 c         1131 g         1181 t
ORIGIN      
        1 tgtcttatgt ctgtttaata attaaacatt caacctccct gcactcagtt gcatttcagg
       61 aaaatattca taaaaatgaa tttgtctgat gttcattaaa cacatccgga tacatgcgga
      121 agtaagatgg taatttaatt tctttaatca tcggatttat tgatacatat ggtgagctgt
      181 ttatttttgg ccgttttggc tcttttatta atcaggtgtt gtcttagtgc ttatgatgta
      241 acgccctgaa tgatatgggc tggttcagat ttgagctgtg atcctgccta gtgtaataga
      301 cttttttatt attgatgctc cttattatat tccataaagg aattgtttgt ttttgttcgt
      361 ttcatacggt ttttagctgt tttatttttt gagaaatcaa catttcacac aaaataaata
      421 aagcatgcgc gagccgctta tataaacata ataaggagct acctgaatcc gcatggaatt
      481 gcccggactg ttgtaaacat attagagagg tagccagatg aatagtattt ccgtttccac
      541 cccggcacca gctgctccgg tgctcccggt tccggctcag caggagcgct tcatacgatt
      601 acctgaagtg atgcaccttt gcgggctatc ccgttcaacc atctacgacc ttatcagccg
      661 cgatgccttc ccgcagcaga tatccctggg tggaaaaaat gtggcctggg tacagtcgga
      721 ggtcagtgca tggatggcgg cgcgaattgc tgcccgcgag caaggaggcc atgcgtgaaa
      781 cttgctttgc agtctcatct tcttttttct ggcttgcgtc ctgatatctt ttccaggtat
      841 agttttcccg ctgccgcaaa atcggctgcc gggcgtagga acccgagtta cttattggcg
      901 acaccagacg cgccgtgcgt ctttttttac gtcgttgctc aggcacaccc atttttcggg
      961 ctgcggtgct tataccttag cccctttcag ataatggtgg tccgggcggg gcagccttcg
     1021 ggctggccgg tttccaataa ggccggtatt cctacccccg ttcgggtcac cacccatgag
     1081 cgtaggaact ccggtggtgg catttaccgc tacttattgg aggttgccct tatggctacg
     1141 atcctcgccc cgtcacatcc tcaatttata tttgtttttg ccgccgttcg tcgtgcagaa
     1201 cgtaaaccac gtatcagtat gctgcgtatc atcgccagca atgagcatca tgcacgtctt
     1261 tcactggtcc atgagtatgt gctttgtctt gctgctcgcc tgcctgtccg ggaggtgatc
     1321 taatgaatca cgccgcaatc tcttatgacg atattctccg cctgaagcat cttcgtaacg
     1381 tgggagagtt tgtcactgga atggctgttc ttcaggattg ctacgaaaaa cctgccagtg
     1441 ctcaatgtga acaactggtt tccctgattt atctgatgac agagcagctt gatggcgtgg
     1501 tacagcgttg ccaggatgac ctcatgaaca tggaggtggt gcaatgaaat cacgcactct
     1561 ttccctttca cttcgggtag cgttgtatcg ccgcgcggta gcctgcgcct ggttgagtgc
     1621 ctgctatcag caaaaccgcc atctgcagct cactctggat gaaattgaaa ccgctattgc
     1681 ccgtgagctt gaaggatttt atctgcgaca gcacgggcaa acaaaaggca tggaaatcgc
     1741 ctgcgccctg ctttcagacc tgatggaata cggccctcta atgtcctgtc ccgcactctc
     1801 ccagctcgga atagccgtaa tggatgagtt atgcgtccgt cacatcaaaa agccggtttt
     1861 acactgaggg agaactgcgt catgtcagga atgaaagtca gtcaggccgt gaaggctgcc
     1921 cgcggtcact gggctcagat tctgccagcg ctgggcgtga atatattgaa aaatcgccat
     1981 cagccttgcc cggtctgtgg cggtaaagat cgttttcgct tcgacgatca ggaaggacgc
     2041 ggaacatggt tctgcaatca gtgcggagcc ggggatggtt tagcgcttgt caccagggca
     2101 ctgaatgtgg atatcagtga agcagctgac aggatacatg gactgacaca tgatctgtct
     2161 gtagttaatt ccgaggtcag gacgttaacc gccgataccg atagcgggaa agatgcagcg
     2221 gcagcacttg ccgcgcgact gctgcaagcc tcgcgtgaat ctgccggaaa cacttatctt
     2281 acacataaag gtttcccgga gcatatttgc catgagctga cttcagctca taaggccggt
     2341 ggtatgatgt tccgccccgg tgatttgatc gtcccactct ataacgctga cggggagctg
     2401 gtgaatattc agctcatcag tggcaatggt agcaagtgtt ttcttaaagg aggtcaggtt
     2461 aaggaggcct atcatctgat agaagggggc gggagttctg taagaaggat ttggattgcg
     2521 gaaggttacg ccacggcgct taccattcat tatttgacag gagaagccgt catggtggca
     2581 ttttcgtcgg tcaactttct ttctctgagc agcgttgccc ataataagta tccgggatat
     2641 cagttaatta ttgcagcgga ccgcgatctg aatggttcag gccagaacag ggctgaaact
     2701 gctgcaaaag cgagtcagtg tgacattgtg ctgccaccgg tttttggtga ctggaatgat
     2761 gcgcttaccc aatacggaga ggaatccacc cggaaggcaa ttcttgaggc tttgaagcca
     2821 cataacgcca gtcctttcga cacaatgagt gaagcagaat tcacagcgat gagtgtcagc
     2881 gaaaaagctc agagggttcg ggagcattac agggatgcac tggcggttga tccgaacggg
     2941 cagcttttat cccgctacga gtctggagcg tggaaagtta tttctcagtc agattttgcc
     3001 cgagatgtcg cagccctttt ccagcgcctc ggggcaccgt tttcctcagg aaaaattgcc
     3061 tcactggtgg aaacattaaa gttaattgtt ccgcagcagc aaaatccgtc acgtcatctg
     3121 attggttttc gtaatggcgt tctcgatacg cgaacagggc tttttagtcc gcactgtaaa
     3181 gagaactggc tgcgtaccct gtgtgaagtc gatttcacgc caccggtaaa gggggaaacg
     3241 cttgaaactc atgccccggc attctggcgt tggctggacc gggccgccgg acacaagcca
     3301 gcaaaacgcg acattattct tgcagcgtta tttatggtgc tggcgaaccg ctatgactgg
     3361 cagctctttc tggaagtgac tggccctggt ggaagtggca aaagtattct ggctgaaatt
     3421 gcgaccatgc ttgctggaga ggataacgcc acctctgcga ccattgaaac gctggagtcc
     3481 tcgcgagaac gtgcggctct gataggtttt tctcttattc gcctgcctga tcaggagaag
     3541 tggagcggcg acggcgccgg gcttaaggct ataactggtg gcgatgcagt atctgtggac
     3601 tccaaatatc agaatgctta ttcaacacat attccggcgg ttatcttggc cgtaaataat
     3661 aatcctatgc gcttcactga tcgcagtggt ggtgtgtccc gcaggagagt tattctgcat
     3721 tttcctgagc agatagcacc ggaagaacgc gatccgcagt tgaaagataa aattgcacga
     3781 gaactggctg tgattgttcg tcagctcatg cagcgtttca gcgatccgat gagcgccaga
     3841 acattgcttc agtcgcagca gaattctgat gaggctctca ccatcaagcg tgatgctgat
     3901 ccagcatttg atttttgtgg ctaccttgag gcattacctg acaccaacgg tatgtttatg
     3961 gggaacgcca atattgtccc acgccagcca cgtacatacc tttaccatgc ctacctcgta
     4021 tacatggagg ctaatggcta taaaaacacc ctgagtctta cgatgtttgg caaagggttg
     4081 ccagtcatgc tgaaggagta cgggctacat tatgagaaac ggcgaactaa tcagggaatg
     4141 caaactaacc tcactttgaa agaggagagt aatgcagact ggctgcccaa gtgtgatgag
     4201 ccaatattaa aataacgatt aactaacccg gctgatgccg ggcttcttat tggaaacgca
     4261 caaatgtaga gatagctgtt cactcttcac ctcgctgtta acacgtaagc aattgatata
     4321 taagttaaaa atggcagggt gaatagtgtg catggttttg cgaaaaaaaa gtattttttg
     4381 tagagtcaag tcc
//
LOCUS       DAZGLJ010000301         4349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000301 DAZGLJ010000000
VERSION     DAZGLJ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.302"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..564
                     /locus_tag="ACHK49_004630"
     CDS             <1..564
                     /locus_tag="ACHK49_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO9265119.1"
                     /translation="IVYPPILSNGMFNFIVMQTLFYLPFFILGALAFIFPHLKALFTT
                     PSRGCTLAAALAFVAYLLNQRYGSGDAWMYETESVITMVLGLWMVNVVFSFGHRLLNF
                     QSARVTYFVNASLFIYLVHHPLTLFFGAYITPHITSNWLGFLCGLIFVVGIAIILYEI
                     HLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(572..2053)
                     /gene="clsC"
                     /locus_tag="ACHK49_004631"
     CDS             complement(572..2053)
                     /gene="clsC"
                     /locus_tag="ACHK49_004631"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO9265120.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(1995..2528)
                     /gene="ymdB"
                     /locus_tag="ACHK49_004632"
     CDS             complement(1995..2528)
                     /gene="ymdB"
                     /locus_tag="ACHK49_004632"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO9265121.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(2623..2934)
                     /gene="ymdA"
                     /locus_tag="ACHK49_004633"
     CDS             complement(2623..2934)
                     /gene="ymdA"
                     /locus_tag="ACHK49_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO9265122.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(3055..3387)
                     /gene="csgC"
                     /locus_tag="ACHK49_004634"
     CDS             complement(3055..3387)
                     /gene="csgC"
                     /locus_tag="ACHK49_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO9265123.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(3446..3901)
                     /gene="csgA"
                     /locus_tag="ACHK49_004635"
     CDS             complement(3446..3901)
                     /gene="csgA"
                     /locus_tag="ACHK49_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO9265124.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(3942..>4349)
                     /gene="csgB"
                     /locus_tag="ACHK49_004636"
     CDS             complement(3942..>4349)
                     /gene="csgB"
                     /locus_tag="ACHK49_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO9265125.1"
                     /translation="GIAAAAGYDLANSEYNFAVNELSKSSFNQAAIIGQAGTNNSAQL
                     RQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDASISQGAYGNTAMIIQK
                     GSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         1084 a         1103 c          980 g         1182 t
ORIGIN      
        1 attgtgtatc cgcccattct gagtaatggc atgttcaatt ttattgtcat gcaaacgctg
       61 ttttatttgc cgttctttat cctcggcgca ctggctttca ttttccctca tcttaaagcc
      121 ttgtttacca cgccgtctcg tggctgtacc cttgcagcag cattggcgtt tgtcgcttat
      181 ttactcaacc agcgctatgg cagtggcgat gcctggatgt acgaaaccga gtcggtgatc
      241 accatggtcc tcggtctgtg gatggtgaat gtggtcttct cctttggcca ccgtttgctt
      301 aacttccagt cagcgcgggt gacttatttt gttaacgcat cgctgtttat ctatctggtt
      361 caccacccgt taacgctgtt tttcggcgca tacattacac cgcacatcac ctccaactgg
      421 cttggttttc tctgtggcct gatatttgta gtagggattg cgataattct gtatgaaatt
      481 catttgcgca tcccgttact gaagtttttg ttctctggta aaccggttgt taagcgtgag
      541 aacgataaag caccagcccg ttaagccaca tttacaataa ccattccacg ggcaatatcg
      601 acgccagtct gaccataacc cgcttccaga aactggtggc gggttctttt ttgagaataa
      661 tctctttctt tgcatgacga tcaacccagt tgatccgtcc ccacctgtcc agacggagct
      721 gccaggccgc atcatactgg ctctgaataa agcgtttatc aattaactgt gccagcgttt
      781 cgctctctat cacgaagccc atttcagtat tgagcaatgt tgaacgcgga tcgaaattga
      841 aagagccgat aaacaccgtt ttaccatcga tgctaaaggt tttagcatgc aggctggctc
      901 cggaattacc ggttatgccg cgatcgtgta acgtactact ttgttcacgc gtcggcttga
      961 gttcatataa ttccacgcca tagcggagca attttttgcg ccagcgcgcg tatccggcat
     1021 ggacgacagc aacatcgtta gcggcaagag aattggttag gatcgcaatc tttacccctt
     1081 ttctcaccat ccgtaagagt tgcgccacac ctgcgcgtgt cggtacaaaa taggaagaga
     1141 taatatcgat gcgttcactg ggtgagccca tgatatcgaa caggcgctgc ggtaacagtg
     1201 aatgccgttt tgccttgccc tcccctttcg ccggatcatc acttaataaa cgtgttttcg
     1261 cccagataag cggcaacgtt ccatcaacca gatgatttat aaacggactg gattccattt
     1321 tgcgtaagta acgatgcgtc atagcatcgt tatgccagga ggcgggtaac tcgatgcgat
     1381 ccgccatttc accttccggg acatccagca cttgctgtaa gggtgaaacc gatttgcaat
     1441 accagtagcg ggcgaaatca tcggcaacgt cctctaccac gggccctatt gccataacat
     1501 ctaaatccga aaaaagtggc tcttcccctg ccccaaaata ggcatcacca atatttcgcc
     1561 cccctaccag cgtcaccacg ccatcgacag tgaaactttt attgtgcata cggcgattga
     1621 gacgggaaaa gtcggtgata taaccaagcg gacgcagcaa gcgaaacgag aaaggattaa
     1681 aaagccggac ttcaatgcgg ggatggctgt caagcaagcg taaaatgtcg tcaagtccgg
     1741 gcgtattgtt gtcgtccagc aacaaacgga cgcgaacgcc acgctttgct gcggctaaca
     1801 gggcggaaaa cagtaaccga cccgacatat cgtcctgcca gatgtaatac tgaacatcca
     1861 gcgtatgctc tgccatttcg gccagacgat accgggcggc aaacgcatcc agacttttct
     1921 ccagagggaa aagaccacac tgaccgggat gttgcgaaca cagtggcagc accgcgctcg
     1981 ccagccgggg caaatcattc atctccttgt tgggtaagga gtctttcgta gaggtgggcg
     2041 ttttcttcat cataacagac aaagtatacc tgttcgggta aagcgtgacg ggtaataaat
     2101 tctgaaacgg ttttaactgc gatttcagcc gctgccgcac gagggtaacc ataaacccca
     2161 gtactgattg caggaaaagc cactgacgta tagctgtttg ccgccaccag tcgtaggcta
     2221 ttgagatagg catcctgcaa aagctggtct tcgttttgtt caccaccacg ccagactggc
     2281 ccgacggtgt gcactacggc tttagcggga agagcgcctg caagcgtaat aacggcatgc
     2341 cccgtagggc aatcgccctg ctgttgcctg acttttaaac aggcatccag cagggccgga
     2401 cccgctgcgc gatgaatggc cccatcgacg ccgccgcctc ccattaatga cggattagcc
     2461 gcattcacaa tcacatcaac ggccagtttg gtaatatcac cctgcacaac atgaatacgc
     2521 gttttcataa cgcctcctta caccttcttg tttcttaagt gtacagcagg ctttttggtt
     2581 aaatcgcatt tttctgctca gcaacgtgcg ttgttcagaa acttagttat attcgatgtt
     2641 cattacagcc agctttttct gtttatcaag atactgcact tttactgagg ctatttgcgc
     2701 gtttttaaca ttaccgctca ttaacgcttt gctggaatag gttttgcccg gaacagaacc
     2761 attctgtggg cagttcattt ctacgggtga tgactgggtg ctgacgtcac atgccggttc
     2821 cacaatttgg ccataaaaat ggatcacccc cccttgcgcg gtgcttcctg cgatggcaat
     2881 aaaaggaaaa agcaaactgc cgagcataag agagtttaaa aaaggtctga acatttattc
     2941 tatttcctca atgattagtc atccttgagg gttgtgttat ccatactttc aggttggctg
     3001 attcgcagca gaccattctc tccagattca tcttatgctc gatatttcaa caaattaaga
     3061 cttttctgaa gagggcggcc attgttgtga taaatgaagt gactgtccat cagaaacagt
     3121 aacaactatt ttcacccgat cttccgggga aatatttaaa ctcaacttcg tcaaagcaat
     3181 aggttgatta gcaggcaatg agagggtctt ttcttgcttc gtctgacttt gccctgaact
     3241 gccttcgcgc agggacaata tttgtactct gcacagacaa gattgagtaa gagtgacttc
     3301 aggaataatg gtatacacat ccccttgctg ggtcgtatta aaggttatct ggctggaaag
     3361 tgccgcaagg agtaataacg cattcatatt cttctcccga aaaaaaacag ggcttgcgcc
     3421 ctgtttctgt aatacaaatg atgtattagt actgatgagc ggtcgcgttg ttaccaaagc
     3481 caacctgagt cacgttgacg gaggagttag atgcagtctg gtcaactgca gcaccgttgc
     3541 caccaccgaa ctgtttaaca gtcatttcag aattttttcc gttccactga tcaagagtgg
     3601 cgctgttacc gaagccacgt tgggtcagat cgattgagct gtcatctgag ccctgaccta
     3661 catctgcacc attaccaccg ccatgctggg taatagtcaa gtcagagtta cgggcatcag
     3721 tttgcagagc aagtgcagag ttaccgccac cgtactggta aatgttcagc tctgaatttg
     3781 ggccgctatt attaccgcca ccaccgtggt taccgccgcc gccgtactga ggaacaacac
     3841 ctgccagagc gctaccggag aatacgattg ctgcaattgc tgctactttt aaaagtttca
     3901 tgtaaaaccc ccatcggatt gatttaaaag tcgaatggaa attaacgttg tgtcacgcga
     3961 atagccattt gcgactgtct ctgcactaca attgccgttt tttgagtacc atactgtgta
     4021 atatttgctt tattaccaga acctttctgg ataatcatcg cagtattacc ataagcacct
     4081 tgcgaaatac tggcatcgtt ggcactgccc gcctgatcaa tatatgcaag gttataatct
     4141 cctgtctggt caatctttgc ccggttgcta ctaccttctt gcgcaacaac cgccaaaagt
     4201 tttgagcctc cctgccgtaa ctgagcacta ttattcgtcc cagcttgacc aattatggct
     4261 gcctgattaa atgaagactt actcaattca tttaccgcga agttatattc tgaattagct
     4321 aaatcataac ctgctgcggc tgcaatccc
//
LOCUS       DAZGLJ010000302         4277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000302 DAZGLJ010000000
VERSION     DAZGLJ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.303"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            99..350
                     /gene="rem"
                     /locus_tag="ACHK49_004637"
     CDS             99..350
                     /gene="rem"
                     /locus_tag="ACHK49_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFO9265126.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDARVTDGFILREGEFVTSLQALKEIGIKAGFSAFSGE"
     gene            417..695
                     /locus_tag="ACHK49_004638"
     CDS             417..695
                     /locus_tag="ACHK49_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265127.1"
                     /translation="MARNAKYYNSDNSPVLACTHGRYSHAFKSEWFQHPPCTAEQAEW
                     LIHSYRRRGFEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            697..1741
                     /locus_tag="ACHK49_004639"
                     /pseudo
     CDS             697..1741
                     /locus_tag="ACHK49_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
     gene            1755..2507
                     /locus_tag="ACHK49_004640"
     CDS             1755..2507
                     /locus_tag="ACHK49_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO9265128.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(2785..2874)
                     /locus_tag="ACHK49_004641"
     CDS             complement(2785..2874)
                     /locus_tag="ACHK49_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265129.1"
                     /translation="MNNPVCLDDWLIGFKSLCCTLAVIALLII"
     gene            complement(2929..3141)
                     /gene="cspF"
                     /locus_tag="ACHK49_004642"
     CDS             complement(2929..3141)
                     /gene="cspF"
                     /locus_tag="ACHK49_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HFO9265130.1"
                     /translation="MSRKMTGIVKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            3442..3657
                     /gene="cspB"
                     /locus_tag="ACHK49_004643"
     CDS             3442..3657
                     /gene="cspB"
                     /locus_tag="ACHK49_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HFO9265131.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIERGAKGPAAANVIITD"
     gene            complement(4021..4191)
                     /gene="ynfU"
                     /locus_tag="ACHK49_004644"
     CDS             complement(4021..4191)
                     /gene="ynfU"
                     /locus_tag="ACHK49_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein YnfU"
                     /protein_id="HFO9265132.1"
                     /translation="MSERKDSKSRRNYLVKCSCPNCTQESEHSFSRVQKGALLICPHC
                     NKVFQTNLKAVA"
BASE COUNT         1195 a          867 c         1053 g         1162 t
ORIGIN      
        1 ggacggtgcc ggaatagaat caaaaatact taagtagcgc gcagggataa gagggatggt
       61 cccttaaagg ggagagctaa ttatccggaa ggattctgat gatgaacatc gaagaactgc
      121 gtaaaatttt ttgtgaagat ggcctctatg ctgtgtgcgt tgaaaatgga aatcttgtta
      181 gtcattaccg cattatgtgt ttgcgaaaga atggggctgc gttaattaat tttgtggatg
      241 ctcgggtcac ggacggattt atcttgcgcg aaggtgagtt tgtcacttca ttacaggcat
      301 tgaaagagat cggaataaaa gctggctttt ctgctttttc aggagaataa actcatctac
      361 aatcttgcgc ggggctgaac tcccgctgag taacaccgtg ccaccggaga aaaccgatgg
      421 cacgcaacgc aaaatattac aattctgata attcgcccgt tcttgcctgc acgcacgggc
      481 ggtattctca cgcattcaag tctgaatggt tccagcaccc tccatgcact gcagaacagg
      541 ccgaatggct gattcattct taccgcaggc gcgggttcga ggttaagaaa gctctcagtc
      601 tcgactatcg gcactggata atctctgtca ggctgcctta ttccgaacgc ccaccacgtg
      661 cgtcccgcac tttccagcaa cggatctgga ggtaacgtgc gggtattact tagacctgtt
      721 ctggtgcctg agcttgggct ggtggtcctt aagccgggcc gtgaatccat acagatattt
      781 cataatcctc gagtgctggt ggaaccggaa ccaaaaagca tgcgtaatct gccatccgga
      841 gtcgttcctg ccgttcgcca gccgctggcg gaagacaaaa cattgctgcc gttttttagt
      901 aacgaacggg tgattcgtgc tgctggcggc gttggcgcat tgtccgactg gctattacgt
      961 catgttacat cctgccagtg gcctaatggt gattaccatc acactgaaac agtcattcac
     1021 cgttatggta ccggcgcaat ggtgttgtgc tggcactgcg acaaccaact gcgtgaccag
     1081 acatcggaat cactggagct gcttgctcaa caaaatctga cagcatgggt gattgacgtc
     1141 atccgtcacg caataagcgg tacgcaggag cgggaattat ctttggctga attatcctgg
     1201 tgggcggtct gcaatcaggt ggtggatgca ctacctgagg ctgtatcgcg tcgttcgctg
     1261 ggattaccag cggaaaaaat ctgctcggtg taccgcgaaa gcgacatcgt accgggagag
     1321 cagaccgcca ccagcatatt gaaacaacgc acaaaaaatc ttgcaccgtt gccttacgcc
     1381 caccagcaac aaaaatcacc acaggaaaag acggtggtaa gcatcaccgt tgatccagag
     1441 tctccggaat ctttcatgaa gctgcctaaa cgtcgccgct gggttaagga gaaatacaca
     1501 cgttgggtta agacacagcc gtgtgcttgc tgcggtatgc cagccgacga tccgcatcat
     1561 ctgattggtc acgggcaggg cggaatggga acaaaagcac atgatctctt tgtgttgcct
     1621 ttgtgcagaa agcatcacaa cgagctgcat acggatacag tggcatttga agataagtat
     1681 ggctcccaac tggatatttc gttttatcga tcgcgcgctg gcaattggcg tactggcgta
     1741 agtggagaac gagcatgaac cttgaagcct taccaaaata ttactcccca aaatctccaa
     1801 aattgagcga tgacgctcca gcgacaggca ccggttgttt aacaattacg gatgtaatgg
     1861 cagcgcaggg gatggtgcag tcgaaagcac cacttgggtt ggccttattt ctggcaaaag
     1921 ttggtgttca ggaccctcag tttgcgattg aaggcctgct aaattacgcg atggcactgg
     1981 ataacccgac attgaacaaa ttgagtgaag aaatccggtt acagattatt ccttacctcg
     2041 tgaattttgc ctttgctgat tactccaggt ctgcggcaag taaggctcgc tgtgagcatt
     2101 gttcaggtac gggattttat aatgtattgc gcgaagtggt gaaacactac cgacgcgggg
     2161 aatctgtaat caaggaagaa tgggtgaagg aactatgtca gcattgccat ggtaagggtg
     2221 aagtcagcac agcgtgcaga gggtgtaagg gtaaagggat tgttctggat gaaaaaagaa
     2281 cccggtttca tggcgtaccg gtatataaga tttgtgggcg ttgtaatgga aaccggttta
     2341 gtcgtttacc gaccacgctg gcacgacgtc atgtccagaa gctggtacca gacctgaccg
     2401 attatcagtg gtataagggg tatgcggacg tcattggtaa actggtaaca aagtgctggc
     2461 aggaagaagc atacgcggaa gcgcaattga ggaaggtgac gagataaatg atttttgctg
     2521 aagatggcga catgatgttt gcatttttca aaaaatatgg ataaaatttt ttcaacgatg
     2581 ggctttgtat acccgacgtt aagaaaaagt agaaaacccg ctgatgagcg ggttttgtgc
     2641 tttaaatggg gcaatggtaa tgttgaatct catcccggga ctcatgtctg ttaacttatt
     2701 atttagctgg tgacttggtt atttgcctga tgtttaaaat gttttcttcc agtacaatgt
     2761 ccctaaacac aatgagtctg cttattatat tattagcaga gctattacgg ccaaagtaca
     2821 gcataagctt ttaaagccaa tcaaccagtc atcaagacag acggggttat tcataaaaac
     2881 tctccatgtg tgatccgatg gggcctgaaa ttaaagcttt aatatagctc atgaaaggta
     2941 aacattggca gctgaagggc cacgcagacc atttatccgg caaaattcca cgcgtaatcc
     3001 ggtggtaatt tcttctgcat cgcggagatt gagcgctgaa acatgaagct ggacatcgat
     3061 acgaccatcg gatggggtga taagaccctt gccgcttttg ccgtcaaagg ttttgacaat
     3121 tcctgtcatt ttacgggaca aaaaaattcc ttaatactga taacttggcg cactatacac
     3181 acgttcctga agaaagctat agttttttga tggggttgaa gatggctgga tgtctaaaat
     3241 aaacattgct tcatatgttc aactatgcgt taatgattgc gtcggtttga agaacagacg
     3301 atatacgaag tagtttacta aagcagttct catttcaggt gttattcact tattccttct
     3361 ttgagtctct ccaattaagt acgaagtcgt ttctgttatg caaaccattt atgccgaaag
     3421 gctcaagtta aggaatgtag aatgtcaaat aaaatgactg gtttagtaaa atggtttaac
     3481 gctgataaag gtttcggctt tatttctcct gttgatggta gtaaagatgt gtttgtgcat
     3541 ttttctgcga ttcagaatga taattatcga accttatttg aaggtcaaaa ggttaccttc
     3601 tctatagaga gaggtgctaa aggtcctgca gcagcaaatg tcatcattac tgattaaaat
     3661 tcatcgctcg tctgtatacg ataacgaaga aggctgatgc ctgagtagag atacggacag
     3721 agtagtgaat attggatctc tttaataaaa agtaaggagg tccaatacat gaaacaatgg
     3781 ctagcatatt tggcaaaatc ttaatcagga aaagtatgct aaccattgtg gtgaagtgca
     3841 ggtttgctgc atgaatagtt tcacagcaga agctaactgc tggcatcgca aaacaaagtg
     3901 cgtaagtgga tgactcccac aaaaagcacc acaatctcaa acccgctcag gcgggttttt
     3961 tattatctgc tttaaatata ttattaaaat ataaaaaata cttgttacta ataaaatcaa
     4021 tcaggctaca gctttaagat ttgtctggaa tactttgttg caatgagggc agatcaaaag
     4081 ggcacctttt tgtactcttg aaaaactgtg ttctgactct tgggtgcagt ttgggcagga
     4141 acatttaacg agataattac ggcgtgattt tgagtcttta cgttctgaca taggcttttc
     4201 ctgtataatg gccgtataca gtacactaaa tatgaaaaca tttctcgtat tattatttta
     4261 tatatgactt tctttca
//
LOCUS       DAZGLJ010000303         4262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000303 DAZGLJ010000000
VERSION     DAZGLJ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.304"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..356)
                     /locus_tag="ACHK49_004645"
     CDS             complement(<1..356)
                     /locus_tag="ACHK49_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO9265133.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQP"
     gene            complement(468..773)
                     /locus_tag="ACHK49_004646"
     CDS             complement(468..773)
                     /locus_tag="ACHK49_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9265134.1"
                     /translation="MRRTNHRNLVNVGILSGRIPLISLVQFIAVAEHLNFRHAAKALG
                     ISQSSVSARVKALEDNLGVLLFERHARGVRLTDAGRHFMERVTAGVDQLDHAVKTAE"
     gene            complement(801..2015)
                     /gene="floR"
                     /locus_tag="ACHK49_004647"
     CDS             complement(801..2015)
                     /gene="floR"
                     /locus_tag="ACHK49_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol/florfenicol efflux MFS
                     transporter FloR"
                     /protein_id="HFO9265135.1"
                     /translation="MTTTRPAWAYTLPAALLLMAPFDILASLAMDIYLPVVPAMPGIL
                     NTTPAMIQLTLSLYMVMLGVGQVIFGPLSDRIGRRPILLAGATAFVIASLGAAWSSTA
                     PAFVAFRLLQAVGASAMLVATFATVRDVYANRPEGVVIYGLFSSMLAFVPALGPIAGV
                     LIGEFLGWQAIFITLAILAMLALLNAGFRWHETRPLDQVKTRRSVLPIFASPAFWVYT
                     VGFSAGMGTYFVFFSTAPRVLIGQAEYSEIGFSFAFATVALVMIVTTRFAKSFVARWG
                     IAGCVARGMALLVCGAVLLGIGELYGSPSFLTFILPMWVVAVGIVFTVSVTANGALAE
                     FDDIAGSAVAFYFCVQSLIVSIVGTLAVALLNGDTAWPVICYATAMAVLVSLGLVLLR
                     LRGAATEKSPVV"
     gene            complement(2232..3116)
                     /locus_tag="ACHK49_004648"
     CDS             complement(2232..3116)
                     /locus_tag="ACHK49_004648"
                     /inference="COORDINATES: protein motif:HMM:NF023271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /protein_id="HFO9265136.1"
                     /translation="MLRSQRRGRDPPCWLSEKEPLVGKRRVNFQRCWLPVSRIGLHRR
                     GVERVNCGPKLLDLGEKGVYSSYTCRVPGGDYEGYVDAHVRRLEALRRAGIVERIDAD
                     QWRIPDDLVSRAAAHDAGRDSQASVRVLSPVDLNKQIGSDGATWLDRRLIHGETADLA
                     PTGFGQQVREAMDQRREHHIEQGDATRSRDSRVFYRRNLLAILREREVAGVGSDMALS
                     KGLPFRAATDGESVSGKFTGTVHLSSGKFAVVEKSHEFTLVPWRPIIDRQLGREVMGI
                     VQGGSVSWQLGRQRGLER"
     gene            complement(3147..>4262)
                     /locus_tag="ACHK49_004649"
     CDS             complement(3147..>4262)
                     /locus_tag="ACHK49_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HFO9265137.1"
                     /translation="FPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKKAGHTHQVAKT
                     GAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPELTQLTHTIAH
                     RVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQAGRKVFTLQT
                     LPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSEKRLSLTRGGN
                     VRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPNSRHRALVTPA
                     KRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAMKVIACIEDPI
                     VIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
BASE COUNT          902 a         1319 c         1231 g          810 t
ORIGIN      
        1 ggttgttcag gcagtacttc atcaaccagc aaggcggcac tttcggccat ccgccgcgcc
       61 ccacagctcg ggcagaaacc gcgacgctta cagctgaaag cgaccaggtg ctcggcgtgg
      121 caagactcgc agcgaacccg tagaaagcca tgctccagcc gcccgcattg gagaaattct
      181 tcaaattccc gttgcacata gcccggcaat tcctttccct gctctgccat aagcgcagcg
      241 aatgccgggt aatactcgtc aacgatctga tagagaaggg tttgctcggg tcggtggctc
      301 tggtaacgac cagtatcccg atcccggctg gccgtcctgg ccgccacatg aggcatgttc
      361 cgcgtccttg caatactgtg tttacataca gtctatcgct tagcggaaag ttcttttacc
      421 ctcagccgaa atgcctgccg ttgctagaca ttgccagcca gtgcccgtca ctccgcggtc
      481 ttcactgcgt gatcgagttg atcgacaccc gccgtgacac gctccatgaa gtgcctgcct
      541 gcgtctgtta gccgaacgcc ccgcgcatgg cgctcaaata gcaggacacc aaggttatcc
      601 tccagcgctt tcacacgcgc gctgacgctc gactggctga taccaagtgc cttggccgca
      661 tgccgaaaat tcagatgctc ggcgacggcg atgaactgaa caagggaaat gagcggtatc
      721 ctgccagaca ggataccgac attcacgagg tttcgatggt tagtgcgccg catcggagcg
      781 ggcctgctac cagtcgtcgg ttagacgact ggcgacttct cggtggcagc cccacggagc
      841 cgaaggagca ccagccccaa cgaaaccagt accgccatcg ccgtggcgta acagatcacg
      901 ggccacgctg tgtcaccgtt taaaagtgcc accgccaatg tcccgacaat gctgactatc
      961 aggctttgaa cgcagaagta gaacgcgacc gctgatcccg cgatgtcgtc gaactctgcc
     1021 aaagcgccgt tcgcggtaac ggacaccgtg aagacaatac cgaccgcgac aacccacatc
     1081 ggtaggatga aggtgaggaa tgacggcgag ccgtaaagtt cgccgatccc caacaggacc
     1141 gctccgcaaa caagcaacgc catcccacgc gccacgcatc ctgcgatgcc ccatctggcg
     1201 acaaaggact tcgcgaaacg ggttgtcacg atcattacaa gcgcgacagt ggcgaaggca
     1261 aagctgaatc cgatctcgga atattccgct tggcctatga gcacacgggg agccgtcgag
     1321 aagaagacga agtaggtgcc cataccggcg ctaaagccga cagtgtaaac ccaaaaagcc
     1381 ggactcgcga agatcggcaa gacagatcgg cgcgtcttga cttgatccag agggcgggtt
     1441 tcgtgccacc tgaaacccgc atttaggagt gcgagcatcg ccagtatagc caaagtaatg
     1501 aatatcgcct gccatcccaa gaactcgccg atcaatactc cggcgatagg gccgagcgca
     1561 ggcacgaacg ccagcatcga actgaaaagg ccgtagatga cgacaccctc aggacggttg
     1621 gcataaacgt cgcgaaccgt cgcgaacgtc gccaccagca tggccgacgc gcccactgct
     1681 tgaagtagac ggaaagcgac aaaggccggt gcagttgaag accaagctgc tcccagagac
     1741 gcaatgacga aagccgttgc gcccgcaagt agaattggcc gtcgcccgat tctgtctgag
     1801 agcggaccaa aaatcacctg gcccacgccg agcatcacca tatagaggct caacgtgagt
     1861 tggatcatag cgggcgtcgt gttcaggatg ccgggcatcg ctggaacgac agggagataa
     1921 atatccatcg ccagtgaagc gaggatgtcg aaaggagcca tcagcagcag tgctgccggc
     1981 agcgtatagg cccacgcggg gcgtgtggtg gtcatgacga atcaaccctc gattaaggaa
     2041 taccgggcga cgtctgctcg tcagcaatca gatgagacta gccttacaga gcgccgcaac
     2101 aacaatactg gttgttgcgg cttacttgtc tgctgacttg gaatttccca tctgattact
     2161 ccacgcttac gaatatgaat tgctacattt tatccgatta tcttttgctt cgcaatgcgg
     2221 catgggcgca ctcagcgttc cagccccctc tgccgcccta actgccacga caccgacccg
     2281 ccctgcacga tgcccataac ctcgcggccg agttggcggt cgatgatcgg ccgccacggg
     2341 acaagggtga actcatggga tttctcgacc acggcgaact tgccgctcga tagatgcacg
     2401 gttccggtaa acttgccgct gacgctctcg ccgtccgtgg cggcgcggaa cggcaggccc
     2461 ttactcaaag ccatatccga tccgacgccg gctacctcgc gctcccgcag gatggcgaga
     2521 aggttgcgcc ggtagaagac gcggctgtcc cggctgcggg tggcgtcgcc ctgttcgata
     2581 tggtgctcgc ggcgctggtc catggcttcg cggacttgtt gcccgaagcc ggttggcgca
     2641 aggtcggccg tctcgccgtg gatcagccgc cggtccagcc aggtcgcgcc gtccgatccg
     2701 atctgtttgt tcagatcgac cggggaaagg acgcgaacgc tggcctgact gtctcggccg
     2761 gcgtcatggg cggcggcacg gctgaccaga tcatcgggga tgcgccattg gtcggcgtcg
     2821 atccgctcga cgataccggc ccggcgtagc gcctccagcc ggcgcacatg ggcatcgaca
     2881 tagccctcat agtcgccgcc tggaacgcgg cacgtatagg aagaataaac gcccttttca
     2941 cccaagtcca acagctttgg accgcagttg actctttcga cacccctgcg atgcaaccca
     3001 atccggctga cggggagcca gcaacgctga aaatttaccc tcctctttcc cactagcggc
     3061 tccttttccg acaaccagca cggcggatcc ctgccgcggc gctgtgaacg cagcattttg
     3121 attggtatcg ttggccttca ggctcgtcag tcaaacagac ccaggagcag ctcagccggt
     3181 ggcgcccggc tttcgggtaa cgccctggtc ccgctggttt cggctttgtg cttcaggtga
     3241 tcaaggatct gcttgatcac tatagggtct tcaatgcagg cgatgacttt catggcgccg
     3301 ccgcagccgc tgcaggtctc gatgtcgata ttgaaaacac gcttgagccg ttgcgcccat
     3361 gtcatcgacg ctcgccgttg tgctggtgtt gccggttcat cagccaccct gaccttgttg
     3421 cccctgcccc gttttgccgg cgtgaccaac gcccggtgcc gactgttggg tgcgaacacc
     3481 ccgtggaagc gggttaggtt gactctgggc ttcggtacca gggcggccag ccttgcaatg
     3541 aaatccaatg gttcgaaaat gacgtgcgtg gtgccgtccc ggtacggcgt cttgagctgg
     3601 tagcgcacgt tgccgcctcg tgttaacgac agccgcttct cggataccgc cgggcggctg
     3661 atgtaccggc acagccgttc gagcttcttg cgttcatcgg ccctggccgc cacgccggcg
     3721 tgcaggctgg acccggctac cttgccaatc ccgtcaccga acggatcacc actggtcggc
     3781 agagtttgca aagtgaacac ctttcgcccc gcctgtgaac cgacagcgat acggtaagtg
     3841 atcgagtgcc ccagcagggg tgtcatcggg tcgtcatcca ccgcatccga ggccagatag
     3901 ctgttttcga catcccgttc cagcaggcct tgccgttcca gatagcgacc cacccggtgg
     3961 gcgatggtgt gcgtcagctg ggtgagctct gggctggtcg gcgccttgac ccagcggaaa
     4021 cgcgctgagc cgtgggattg ctcgacatac acaccgtcga gaaacagcat gtggaagtga
     4081 acattcagat tgagcgccga tccaaaacgc tggatcaggg tgaccgcgcc cgtcttggcc
     4141 acttggtggg tatggcccgc tttcttgacc aggtgcgtgg caatgacgcg gtaaacgatg
     4201 cccagcaccc accccatgat ctcgggccgg ctggcaaaca ggaaacgcag ctgaaacggg
     4261 aa
//
LOCUS       DAZGLJ010000304         4172 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000304 DAZGLJ010000000
VERSION     DAZGLJ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.305"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..102)
                     /locus_tag="ACHK49_004650"
     CDS             complement(<1..102)
                     /locus_tag="ACHK49_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO9265138.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKVEEELKREGH"
     gene            complement(99..521)
                     /locus_tag="ACHK49_004651"
     CDS             complement(99..521)
                     /locus_tag="ACHK49_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO9265139.1"
                     /translation="MAKVFTQEEREKIKEQVVELVRQSGRETLRQLEAKTGATRYLMS
                     ILARELVASGDVYNSGYGLFPSEQARKDWKNARKKLSRAKVKKTVVVDPDLIWSLPDG
                     EIRRYDRRQNIICRESRRSEVMQRVLAFYRGNFQEVME"
     gene            complement(537..1298)
                     /locus_tag="ACHK49_004652"
     CDS             complement(537..1298)
                     /locus_tag="ACHK49_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO9265140.1"
                     /translation="METVFDALKAMGKATSIELAARLDISREEVLNELWELKKAGFVD
                     KSAYTWRVADNNVQQEQPMQAELPEETTTATVVKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKFRYCMPGDNLPAEPKAASVAETDGKAFPQ
                     PAGVALPVQEAATQEDIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKNH
                     VQKWERVCAALRELNKHRDIVRQIVDSSSRIVSEK"
     gene            complement(1321..2067)
                     /gene="ydaV"
                     /locus_tag="ACHK49_004653"
     CDS             complement(1321..2067)
                     /gene="ydaV"
                     /locus_tag="ACHK49_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO9265141.1"
                     /translation="MKNIATGDVLERIRRLAPSHVTAPFKTVAEWREWQLSEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFSNYQVQNEAQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVVQPGIEK"
     gene            complement(2074..2931)
                     /gene="ydaU"
                     /locus_tag="ACHK49_004654"
     CDS             complement(2074..2931)
                     /gene="ydaU"
                     /locus_tag="ACHK49_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFO9265142.1"
                     /translation="MLFVLILSHRAASYGAIMAALPYMQLYIADYLADTMHLSAEEHG
                     AYLLLMFNYWQTGKPIPKNRLAKIARLTNERWADVEPSLQEFFCDNGEEWVHLRIEED
                     LASVREKLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVEVVFEHDVNTKAT
                     NKDTDKDLKTDPPLNPPRGNRGVKKFDPLDIALPNWISVSLWREWVEFRQALRKPIRT
                     EQGANGAIRELEKFRQQGFSPEQVIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPD
                     SAIPPGFRG"
     gene            complement(2944..3366)
                     /gene="ydaT"
                     /locus_tag="ACHK49_004655"
     CDS             complement(2944..3366)
                     /gene="ydaT"
                     /locus_tag="ACHK49_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO9265143.1"
                     /translation="MKIKHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKTQREKIQKLIPAILAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVIVH"
     gene            complement(3363..3617)
                     /locus_tag="ACHK49_004656"
     CDS             complement(3363..3617)
                     /locus_tag="ACHK49_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9265144.1"
                     /translation="MNQKTLEDVIKTVRVSVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIAHASNGLFSADVILTIGRNKTTTKKLMGVDS"
     gene            3697..4116
                     /locus_tag="ACHK49_004657"
     CDS             3697..4116
                     /locus_tag="ACHK49_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9265145.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQVRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
BASE COUNT          989 a         1121 c          897 g         1165 t
ORIGIN      
        1 atgcccttcc cgtttcagtt cctcttccac cttgtgaaat gcctcacggt tgaaattttc
       61 atatcccgtc attggaccgg caatataaac tctcaccctc actccatcac ctcctgaaag
      121 tttccccgat agaacgccag cacacgctgc ataacttcgc tcctcctgct ctcacgacaa
      181 attatgttct gacgcctgtc atagcggcgt atttctccgt ctggtaatga ccagataagg
      241 tcaggatcaa ccacaaccgt ttttttcacc tttgccctgg atagtttttt gcgggcgttt
      301 ttccaatcct tacgagcctg ttcagacggg aataacccat agccagagtt gtatacatcg
      361 ccactggcaa ccaactctct ggcaagaatg ctcatcagat atcttgtcgc acctgtcttg
      421 gcctccagtt gccttaacgt ctcgcgccca ctctggcgta ccaactcaac aacctgctcc
      481 ttaatttttt cacgctcttc ctgtgtaaaa acttttgcca taagcgcctc cggcaatcac
      541 ttttccgaca caatacgact ggaggaatcg acaatctgtc ggacaatatc ccggtgcttg
      601 ttcagctccc gcagcgcggc gcagactcgc tcccacttct ggacatgatt tttcgcccga
      661 cgcagttcgc ggtttgccat atgcagcgat ggtaaaacta ggtcatccgc ttgcgtttca
      721 gtaaacgatg gcagcgactg cacaatgtcc gccacagttt ctgttttaat atcttcctgt
      781 gttgccgctt cctgtactgg taacgcaaca ccggctggct gaggaaaggc tttaccatca
      841 gtttccgcta ccgatgcagc tttcggctct gctggtaaat tatcgcccgg catgcagtaa
      901 cgaaatttac cgttctggtt tacgcgaatc aggcgtcctt tgctgattgc cattgccagc
      961 gttgaagcaa ctttgcggga tgttgtaccg aacagcgtag ccagttcatc cgccgtttgt
     1021 ggtccgcgtt gttcaatcgt cgcggttaaa tcgcactctg agattttcac tacagttgcc
     1081 gtggtggttt cttccggcag ttctgcctgc attggctgtt cctgctgaac gttgttatca
     1141 gccacacgcc aggtgtacgc gcttttatca acgaaaccag cttttttcag ttcccatagt
     1201 tcgttcagca cttcttcacg actgatatca agtcgcgcag caagttctat ggatgtggct
     1261 tttcccattg ctttcagtgc gtcaaaaaca gtctccataa aaatttcctc ccggtaaaaa
     1321 ttacttctca attcctggct ggacgacatt cggacgccag ctctcccagt taaaattcac
     1381 ccatcgcccg ccgttcatgg tcatgcgatc cataatccgc tcgccgagca atgttttcat
     1441 ggcctcatag ttcaggtttg tcagcatccc cacgctgcgc atcgacgctg tccggcgatc
     1501 aacaatctgg tgcagcacca cctgctcgtt tttcgtctcg cgctgaatgc caatttcatc
     1561 aagaaccagc agatccactt cgcatagttc ccgcaaaaat ttttcgcctg actgcccatc
     1621 gtcatagctg gcgtgcaggg cactcataac atcagccacg gtaaccacaa tcactgtctg
     1681 accgtctttc agcaggcgat tcccgatagc tgccgctaag tggttcttcc cggtaccagg
     1741 ttttccgctg aacgcaaaat ttgtacaccc ggtcatcagt tcatcagcga tggatttcgc
     1801 ctgactcaac gcgtatcgct gcgcttcgtt ctgcacctgg taattcgaaa acgagcattt
     1861 gcggtgcaat ggctggatgc cagagcgatt cagaattttt tccacccgca actgacgatt
     1921 ctgacggttg atctcctcac aacgtttctg gccttcggaa agttgccact cgcgccactc
     1981 cgctaccgtc ttgaatggcg cggttacatg tgacggggcc agtctgcgga tacgttcaag
     2041 aacgtcgcct gtcgcaatat ttttcatggt cagttacccc ctgaagcctg gcgggatcgc
     2101 actatccggt aacgagacgg tgttaacctg tcggagtaac gtctcaggtc gaacaccttt
     2161 cggcgcgaac aagccctggt attcattggc gatgctgtgt cgaatcacct gctcaggtga
     2221 aaaaccctgc tggcggaatt tttccagctc ccgtatcgcc ccgttagcgc cctgctccgt
     2281 tcgaatcggt tttcgcaatg cctggcgaaa ttcaacccac tcacgccaaa gcgagacaga
     2341 aatccagttc ggcaaagcaa tatccagagg gtcaaacttt ttgacacctc gattcccccg
     2401 ggggggattt agggggggat ctgtttttag atctttatct gtatctttat tagttgcctt
     2461 tgtgttgaca tcatgttcaa acaccacttc aacatctgtt tgaacacctg ttaaatttct
     2521 ctcttgtttt gtttgaacat ctgcttcctt tctgcttctt ctggcctgaa cagatgcttt
     2581 tcctgcggct gattttttgg ttaatttttc cctgactgat gccagatctt cctcaatccg
     2641 aagatgcacc cattcctcgc cgttatcgca aaaaaactcc tgcaaggatg gttcaacatc
     2701 agcccatcgc tcgttagtca gacgggcaat ttttgccagc ctgtttttag gtattggctt
     2761 tcctgtttgc cagtaattga acatcagcaa caaatacgca ccatgctcct ctgctgacaa
     2821 atgcatggtg tcagccaggt aatcagctat gtacagttgc atgtatggta atgcggccat
     2881 aattgccccg tatgatgctg cccggtggct tagaataagc acaaacagca tggaaacttt
     2941 tgcttaatga acaatgacag aatcgtcgga agacccgccg ccgctgaaat gcgctttccg
     3001 gtaaacggct tggactgcat catcatgcgc atcaattgcc gtacttaacg cttcctgcgc
     3061 cgccagtaat gcacggcgtt ccagggtatc gaagatgcag agtcggtgac gcagctcgcg
     3121 cggaaggatt gccagaattg ctgggatcag cttctgaatt ttttctcttt gcgttttcgt
     3181 ttcacctttc aaccaacggt gatagatatt ctgctgatta ttccagtcct tgcctggtac
     3241 caggggcaat tcgccgcccc cctggcgcag atattcttca gtaatggcat tggctaccca
     3301 tgcctgccct ttttcggctg ccagggctaa caacactgac tcgatgtgct catgcttgat
     3361 tttcatgaat caactcccat cagctttttc gtagtagttt tatttctgcc aatagttaaa
     3421 attacatcag cagaaaataa tccgtttgat gcatgagcga ttttttcagc gtaatttgtt
     3481 tcgccggtat attctgtgcg aggcaatttt ccgttatcca tccatttgta gattgctctt
     3541 tggctgacac cacaaacgtc ggccacaaca gaaacgcgaa cagttttgat tacatcttca
     3601 agtgttttct ggttcatatc atcctcacaa tgtgaacttt gagtacatgc tataacagaa
     3661 ctgacagtac attcaagagc gaatatcatt gaacttatgg ttcatgaaga taaagcgcgt
     3721 aaagagttcg ccagtaggct tgcgctagcc tgtgaaaacg ctggttatga acaacatgga
     3781 aggcaggcag aaattgcccg tcgaatgaaa ttaacaccaa aagcggttag caaatggttt
     3841 aatggcgaaa caattcctcg ccgagagaaa ttaagggaat tagcaacact cattggaaca
     3901 acaccaacct atcttttggg agaggataca gaagaaagtg gacaggtacg tttctatcag
     3961 gagttaaatc caagacaaaa aatcatcatt gaccttctgg acgagctccc tgacagtgag
     4021 acagatgaac ttttaaaaac tcttgaggag aaaaaacaga agtacaatgc aatttacgaa
     4081 gagctagcac gaaagaaaaa acaaaaagcc tcttaaacca gcataaatcc ggtagcgtcc
     4141 ccctccgggt ttgtgcttca ctttatccca tc
//
LOCUS       DAZGLJ010000305         4148 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000305 DAZGLJ010000000
VERSION     DAZGLJ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.306"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..944
                     /locus_tag="ACHK49_004658"
     CDS             <1..944
                     /locus_tag="ACHK49_004658"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HFO9265146.1"
                     /translation="KKINKEIFEKIDYPHYLHGALSGRDYISNAAVHTRKRTVICLDI
                     TNFYPSISKKDVCSIFKNLMRFSPEVSLCLTELVTLNNKVPQGGCCSSYIANLLFFNS
                     EYNLYNRLKSMGLSYSRLLDDITISSDKDLSSEEKTKVIKLVHGMVNQYRLSINESKT
                     TIEHSKDSSSKLSVTGLWVKHGVPKLTKENRRYIRYLVYICKKQGAYERHTKEYHDLW
                     NRCSGKVAQMSRLGHVQAVELRAILSEIMPVYDDYKISKLKLMAKHYLNKFTPPLTDE
                     QIRKIDRMLYDFDIVGRTNKNLAKLYRRKLVALLPDR"
     gene            949..1152
                     /locus_tag="ACHK49_004659"
     CDS             949..1152
                     /locus_tag="ACHK49_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retron Se72 family effector protein"
                     /protein_id="HFO9265147.1"
                     /translation="MENGFVNFYDHVKGYGFIRRERGRDVFFRYDDFLFLGHDVDICK
                     GILVRFKLEKTDKGFKAVAIQKV"
     gene            complement(1989..2183)
                     /locus_tag="ACHK49_004660"
     CDS             complement(1989..2183)
                     /locus_tag="ACHK49_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO9265148.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEL
                     AEELQNSIQTRFEAENRTGI"
     gene            2468..2791
                     /gene="mobA"
                     /locus_tag="ACHK49_004661"
     CDS             2468..2791
                     /gene="mobA"
                     /locus_tag="ACHK49_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO9265149.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            3041..3379
                     /locus_tag="ACHK49_004662"
     CDS             3041..3379
                     /locus_tag="ACHK49_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265150.1"
                     /translation="MNISIKNNDLKTIARFDGKTLRDAFSRKIATFDGKILRDASLKK
                     IATFDGKVLRDASLKKVATFDGKVLRDASLKKVATFDGQNLRDASSKIILSADAEVGI
                     VVMAFVAELI"
     gene            complement(3928..>4148)
                     /locus_tag="ACHK49_004663"
     CDS             complement(3928..>4148)
                     /locus_tag="ACHK49_004663"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265151.1"
                     /translation="IPFKDLLGSKICLSFLFFPVSTLNCLYDIDTDLTIQSNLFLSMP
                     NIADKESKDATGVPLNLGLFMMFELKVD"
BASE COUNT         1143 a          779 c          987 g         1239 t
ORIGIN      
        1 aaaaaaaaat aaataaagaa atttttgaga aaattgatta ccctcattat ttgcatgggg
       61 cgttaagtgg tcgcgattat atatctaatg ccgctgttca tactaggaaa agaacagtta
      121 tatgtcttga tatcaccaat ttttatccca gcattagtaa aaaagatgtt tgttctattt
      181 ttaaaaattt aatgcgtttt tctcctgagg tttctttgtg cttaacggaa cttgtgacgt
      241 tgaataataa ggttcctcag ggtgggtgct gttctagtta tattgcaaat cttttatttt
      301 ttaatagtga gtataatctc tataatagat tgaagtctat ggggctttca tattcaaggc
      361 ttttagatga tattactata tcctctgata aggatctttc atcagaggaa aaaacaaaag
      421 taataaaact cgttcacgga atggtgaatc agtacagact ttcaataaat gaaagtaaga
      481 caacgataga gcactcaaaa gattcgtctt ctaaacttag tgtcactggg ttgtgggtaa
      541 agcatggcgt tcctaagtta acaaaagaaa atagacgtta tatcaggtac ttagtttata
      601 tatgtaaaaa acaaggtgct tatgaacgtc atacgaaaga gtaccatgat ttatggaata
      661 gatgctctgg caaggttgct caaatgtcga ggcttgggca tgtccaggcg gttgaattga
      721 gggcgattct ttctgagata atgccagttt atgatgatta caagatatct aaattaaagt
      781 tgatggctaa gcattatcta aataaattta cgccgccttt aactgatgag cagataagga
      841 aaatagatag gatgctctat gattttgata tagtagggcg gactaataag aatcttgcaa
      901 aactatatag acgtaagctt gtggcattac ttccagatag gtgattatat ggaaaatgga
      961 tttgttaatt tttatgacca tgttaaaggg tatggcttta taaggcgtga gcgtggacgt
     1021 gatgttttct ttcggtatga tgattttttg tttttagggc atgatgtcga tatatgtaaa
     1081 ggtattttag ttaggtttaa attagaaaaa acagataaag ggtttaaagc tgtagcgatt
     1141 caaaaggtat aacatcgtgg atgtgtcagc ttgtttttat gatatgagaa cttgagatcc
     1201 tttctatctc gtcgtaatct cttgctgtgt aaacgaaaaa accgccttgc agggcggttt
     1261 ttcgaaggtt ctcagagcta ccaactcttt gagccgaggt aactggcttg gaggagcgca
     1321 gtcaccaaaa cttgttcttt cagtttagcc ttaacaggcg catgacttca aggctaactc
     1381 ctctaattcg gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt ctcggtttgg
     1441 attcaagtcg atagttaccg gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca
     1501 tacagtccag cttggagcga acgacctaca ccgggccgag ataccaacag cgtgagctat
     1561 gagaaagcgc cacgtttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     1621 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     1681 ctgtcgggtt tcgccacctc tgacttgagc gtcggttttt gtgatgctcg tcaggggggc
     1741 ggagcctatg gaaaaacgcc tcctgctcgc ccttcttctg ggcctttgtc ttttgctcac
     1801 atgttctttc cggctttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     1861 gctgacaccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg
     1921 gaagagcgcc ttatgtgaca ttttctcctt acgctcctct atgccgttct gcatcctgtc
     1981 ctgatgcgtt atatcccggt gcgattttcc gcttcaaagc gtgtctgtat gctgttctga
     2041 agttcttccg cgagttcgtg cagcttctca cacatggctg cctgttcgtc agcatcaagt
     2101 gcgtccagtt tttcgagcag cgtcaggctc tggcttttta tgaatcctgc catgttgagt
     2161 acggtttgct gctgcttgtt catctttctg ttttctccgt tctttctgtc atctgctttg
     2221 tgtgattata tcgcgtacca cttttcgact gttttaccgt cggtattctg ctgtctgact
     2281 ctttgacggg catttctgtc agacaacact gtcactgcca aaaaactgcc gtgcctttgt
     2341 cggtaattcg agcttgctga caggacagga tgtgtaattg ttataccgcg catacatgca
     2401 cgctattaca attaccctgg tcagggcttt gccccgacac cccatgtcag atacggagcc
     2461 atgttttatg acaaaacgaa gtggaagtaa tacgcgcagg cgggctatca gtcgccctgt
     2521 tcgtctgacg gcagaagaag accaggaaat cagaaaaagg gctgctgaat gcggcaagac
     2581 cgtttccggt tttttacggg cggcagctct cggtaagaaa gttaactcac tgactgatga
     2641 tcgggtactg aaagaagtta tgagactggg ggcgttacag aaaaaactct ttatcgacgg
     2701 caagcgtgtc ggggacaggg agtatgcgga ggtactgatc gctattacgg agtatcaccg
     2761 tgccctgtta tccaggctta tggcagatta gcttcccggg gagaaaactg tcgaaaacag
     2821 acggtatgaa caccgtaagc tcccaaagtg atcaccattc gctttcatgc atagctatgc
     2881 agtgagctga aaacgatcct gacgcatcct tcctgttttt tcggggctaa acctctcttt
     2941 ttgcggtatt tgcgtcagaa ttgcgttcag cgcgtttcag cggtgcgtac aattaaggga
     3001 ttatggtaaa tacaattggt taaattacta ttggtgataa atgaatattt caataaaaaa
     3061 caatgactta aaaactattg ctcggtttga cggtaaaacc ttaagagatg ccttttcgag
     3121 aaaaatagcg acttttgatg gcaagatttt aagggatgct agcttaaaaa aaatagcgac
     3181 ttttgatggc aaggttttaa gggatgcctc tttaaaaaaa gtggcgactt ttgatggcaa
     3241 ggttttaagg gatgcctctt taaaaaaagt agcaaccttt gacggtcaga atttaagaga
     3301 tgctagttca aaaattatcc tgagtgccga tgctgaggtt gggattgttg ttatggcctt
     3361 tgtagctgag ttgatttgaa tctcatgctg catacctttt agcctatcgc tggcaggata
     3421 aggcgctttc ttgttggtga cagaaagata aaaaacttta taataatcag cgaagcgggc
     3481 gaaccacgcc tggctctcgc tctttaacaa atggttacgt gtatgttgcg caaggaaacc
     3541 gatgccttgt ttttaaggcg atctcgcatg agatcatgac cggcctggcc gctaattaat
     3601 ggtgagaacg cccctgagcg tgtatcagca ctaaaagaat agcgacgatt gtctggcaat
     3661 gtgagttaaa agtcccaaag agccaataga gtttttcctt gctttcctac tggtctggca
     3721 aaatttcata accaatagga gttgcatcat gtttattatc aacatcaaca ttaacgtaac
     3781 caagagctct tcatcttgtt ctggagacgg tcagatgaag aaaaaaagag aggttggctc
     3841 tctggaggca ctgaaaaaaa cacagttttt gttttctatg tgcttgatta tgtttaaagt
     3901 aatagattac gtatcgaaac tttttaacta atcgactttt aactcaaaca tcatgaataa
     3961 acccaggttt aatggaactc ctgttgcatc tttggattct ttatctgcga tgttaggcat
     4021 agaaagaaaa agattggact ggatagtcaa gtctgtatcg atgtcgtaca agcagtttaa
     4081 ggttgaaaca gggaagaata aaaaagaaag gcaaattttt gaacccaaga gatctttgaa
     4141 aggaattc
//
LOCUS       DAZGLJ010000306         4103 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000306 DAZGLJ010000000
VERSION     DAZGLJ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.307"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..351
                     /locus_tag="ACHK49_004664"
     CDS             <1..351
                     /locus_tag="ACHK49_004664"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO9265152.1"
                     /translation="GAYSFMIIAPIVLISKCRDVYRNKILTYVGEISYSLYINHMVVY
                     SLAVLYLKPYGVLDSKGFGVFLAMICATFIISIISYNFIEKPSVSIGHAIARRMQKKS
                     DVSSIKTESVREAP"
     gene            complement(596..1753)
                     /gene="intS"
                     /locus_tag="ACHK49_004665"
     CDS             complement(596..1753)
                     /gene="intS"
                     /locus_tag="ACHK49_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFO9265153.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMRGYRKKNFPFLPADQIPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMQWKNVDFENRIITIDANVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(1915..1989)
                     /locus_tag="ACHK49_004666"
     tRNA            complement(1915..1989)
                     /locus_tag="ACHK49_004666"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1954..1956),aa:Arg,seq:cct)
     gene            complement(2065..2997)
                     /gene="yfdC"
                     /locus_tag="ACHK49_004667"
     CDS             complement(2065..2997)
                     /gene="yfdC"
                     /locus_tag="ACHK49_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO9265154.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3291..4046
                     /gene="mlaA"
                     /locus_tag="ACHK49_004668"
     CDS             3291..4046
                     /gene="mlaA"
                     /locus_tag="ACHK49_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO9265155.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         1006 a          966 c          960 g         1171 t
ORIGIN      
        1 ggggcttatt catttatgat tatagcccca atagtcctta tttctaagtg tagggatgta
       61 tatagaaata aaattctaac gtatgttggt gaaatatcct attcacttta cattaatcat
      121 atggttgtat acagtcttgc tgttttatat ctcaaaccgt acggggtatt ggatagtaaa
      181 ggatttggtg tttttctagc tatgatttgc gctactttta ttatatcaat catatcttat
      241 aactttattg aaaaaccatc agtatccata gggcatgcca tcgctcgtag aatgcaaaaa
      301 aaatcagatg tatcttcaat taaaactgaa tccgttagag aagcaccttg ataaatagag
      361 caatagccag atagcaccta accgatttcg tactcatagg gttgtcactt taccaaactg
      421 agaggaaata cactgataac agttggttat gaagtactgg gtgaggtgga tgtgaatggt
      481 attttgatgc atttcattaa cctgtacgcc taagaaagca accctgaaat ctaagacaca
      541 caagctttgc actggattgc aaggctttgt gcacttcgat agtggttaag gtggttcact
      601 ccaccttttc atcaagccag tccgcccacc attgcatcat ttctctgcgc ttatcgagat
      661 actgagcatg gttgtaaata ccgcgcacag atccgccgtt ggcatgtgcc agttgcactt
      721 caatagcgtc agcaggccat tcgtgctcgt tcataatcgt gctgaattca tgcctgaatc
      781 cgtgaccgct ttccagaccc tcatagccga tttgtttgat cacaagtagc accgcgttct
      841 cgcagattgg cttcttctta tcgttgcgcc cggcaaaaac aaattctgat actggtttgg
      901 tgatggagct taacgtagtg agaagttcaa ccacctggtc cgacatcggg acaacatgaa
      961 ttttgcgtcc cttcatcaca ttggcgtcaa tggtgataat cctgttttca aaatcgacgt
     1021 tcttccattg catggaacga agctcttttg ttcttagggc tgtgtagcgt aaaaccttgg
     1081 tggcaatgag cgatacgata cttcctgaaa atgttgccag tgctttattg aatgccggga
     1141 tctggtctgc aggaagaaac gggaagttct tcttgcggta tcccctcatg gcgtcagcaa
     1201 ggtcaggtgc cgggttatat ttagccctgc cggtgacaat agcgtaacgg aaaacctcgc
     1261 cgcatcttct gcgggcttta tttgctcgct ccattgcacc gcgatcttca aatctgcgga
     1321 ttacttccag cagttgcatc ggctcaatat cctgaatttc aaggccgccg atgataggca
     1381 aaatgtcgtc atcaaacatt tttgcaagtt cagtcgcata ccctactgac catacttgct
     1441 tcttgtgctc gtaccattcc ttgtaaatcg cactaaagga attgttgtta gacgaagcct
     1501 ttttcgcctt taccggatcg atgccaaccg agatgtcttt cctcgcagtc catgccttat
     1561 cccttgcctc ctgcaaagtc attagcggat attttccgac ggtcaggatt ttctccttac
     1621 cgtcaatctt gtagcgaagc tgccatacct ttttcccgga tacaggaaca taaaggtaca
     1681 ggccattacc atcgagaagg cggtatggtt tttctttcgg ctttgctgct tcaatctgct
     1741 taacggtgag catgggtaaa aatccggagg gtaaaattat tttatccact ttttacccgt
     1801 catggggtgc ggctgtcaac gatctgacgc gaaccatgac gaactgtgaa tctacggaag
     1861 gcttgatatt caggggattt tgcggactgg tacggatggg agcgaactga taaatggtgt
     1921 cccctgcagg aatcgaacct gcaattagcc cttaggaggg gctcgttata tccatttaac
     1981 taagaggaca atgcggcatg agtatacccg ctaatggact gcggggtaag tacgctgccg
     2041 ctcgattgct taaaccctcg ccatttatgc tgggttttta tcatttttct taatgttttc
     2101 cgcacgttct gctttttggc gtgcttctgc tttacgcttg ttgctcatgt cgttacgaat
     2161 ctgtgcatga ctcattaacg cgaagataaa ggtgccgccg cagatgttcc ccgctaaagt
     2221 aggtagtgcg aagggccaga tgaaatcgct ccagtgcagc gtgccgttaa acaccagata
     2281 gaggatttca acagaaccga caacgatgtg ggtggtgtca cccagtgcaa taagccaggt
     2341 catcaaaata atcaccacaa tctttgccgc acccgctgca ggaaacatcc aaaccatagt
     2401 ggcgatcagc cagccggaaa tgatcgcgtt ggcaaacatc tcgctggggg tgttcttcat
     2461 cacatccatg ccgattttga caaatgcatc gcgagtttct tcattgaaga taggcatata
     2521 ttcaaatgcc catgcagcaa tacctgtccc gagaatatta cccagcagca cgacgcccca
     2581 taatcgcata agtaagccga cgttgctcat tgtcggtttt tgcatgacgg gtagtaccgc
     2641 agtcacggtg ttttcggtaa ataattgctg gcgggccata atgacgataa taaaaccaaa
     2701 ggtataaccg agattctcca gtaagaagct gcctggcact ccttccagtt cgacatgaaa
     2761 tatccctttt gccagtagcg aagcgcccat cgacagaccc gccgcaatgg ctgaccacag
     2821 tagcgccatt gcgtcgcgtt ccagctcttt ttcaccatcc tggcggatat gctcatgaat
     2881 tgccatcgcc cgggagggga gtcggtcttc atctatttct atttttttgc cgcgctcttt
     2941 ttcttcgctc tcaacttcaa tttcgtcgct gtgttgatca attttgtcgt tgtccatggt
     3001 ctcttctctt gaattagcac gtatagctaa agcgtagcgg cttttttgct cgcaactggc
     3061 gggagttact ctgaaaatgt agaaaaggct gcgtttgcct ttttctgttt ctatagaatc
     3121 aagtagccta cagggcggcg attaccaggc tatgatcaaa tcagcaaatc agggcgtctg
     3181 gacatcagtt gacgtgctgt tacaatcgcc cacacctaaa caggcggata cggtatcgtt
     3241 ccgtcatgga tggcaaactg cataagccat aaaaaaacag ggagacattt atgaagcttc
     3301 gcctgtcggc gcttgctctg ggaactacgc ttctggtggg gtgtgcgagt tccggtacag
     3361 atcagcaagg gcgttctgac ccgttagaag ggttcaaccg caccatgtac aacttcaact
     3421 tcaacgtatt agacccgtat attgttcgac cggtcgctgt cgcctggcgt gattatgttc
     3481 cgcaaccggc gcgtaatggt ttgagcaact ttactggcaa ccttgaagaa cctgcggtga
     3541 tggttaacta cttcttgcag ggcgaccctt atcaggggat ggtccacttt acccgctttt
     3601 tcctgaacac cattttgggg atgggcggct ttattgatgt tgcagggatg gcgaacccga
     3661 aactgcaacg gactgaacct caccgcttcg gtagtacgct tggtcattat ggcgtgggtt
     3721 atgggcctta cgttcagtta ccgttctacg gtagcttcac gctgcgtgat gacggtggtg
     3781 atatggcgga tggtctttac ccggttcttt cctggctgac ctggccgatg tctgtgggta
     3841 aatggacgct tgaagggatc gaaacccgcg ctcagctgct ggattccgat ggtctgctgc
     3901 gtcagtcgtc cgatccttat attatggtgc gcgaagcgta cttccagcgt catgatttca
     3961 tcgctaatgg cggcgaactc aaaccgcagg aaaatccgaa cgcacaagcg attcaggatg
     4021 atttaaaaga tattgattct gaataagaaa caaataaaaa aggtgagtcg caaaactcac
     4081 cttttttgtt gttatccctc aat
//
LOCUS       DAZGLJ010000307         4087 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000307 DAZGLJ010000000
VERSION     DAZGLJ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.308"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            191..1105
                     /locus_tag="ACHK49_004669"
     CDS             191..1105
                     /locus_tag="ACHK49_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO9265156.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            1177..2424
                     /locus_tag="ACHK49_004670"
     CDS             1177..2424
                     /locus_tag="ACHK49_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO9265157.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(2965..3372)
                     /locus_tag="ACHK49_004671"
     CDS             complement(2965..3372)
                     /locus_tag="ACHK49_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO9265158.1"
                     /translation="MSDTLHGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTITLYAKGLTCEAD
                     TLGSCSFVYMAVYATRFSNQLPKASVHQLTHYF"
     gene            complement(3446..3667)
                     /locus_tag="ACHK49_004672"
     CDS             complement(3446..3667)
                     /locus_tag="ACHK49_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO9265159.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(3730..>4087)
                     /gene="radC"
                     /locus_tag="ACHK49_004673"
     CDS             complement(3730..>4087)
                     /gene="radC"
                     /locus_tag="ACHK49_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO9265160.1"
                     /translation="RAAREWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRT
                     EVHPREVIKRALYHNAAAVVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHL
                     IVGGNQVFSFAEHGLL"
BASE COUNT          980 a          879 c         1060 g         1168 t
ORIGIN      
        1 tggtgaattg ttaacgttaa cttttataaa aatatagtcc cttactttca taaatgcgat
       61 gaatatcaca aatgttaacg ttaactatga cgttttgtga tcgaatatgc atgttttagt
      121 aaatccatga cgattttgcg agaaagaggt ttatcactat gcgtaactca gatgaattta
      181 agggaaaaaa atgtcagcca aagtatgggt tttaggggat gcggtcgtag atctcttgcc
      241 agaatcagac gggcgcctac tgccttgtcc tggcggcgcg ccagctaacg ttgcggtggg
      301 aatcgccaga ttaggcggaa taagtggatt tataggtcgg gtcggtgatg atccttttgg
      361 ggcgttaatg caaagaacgc tgctaactga gggagtcgat atcacgtatc tgaagcaaga
      421 tgaatggcac cggacatcca cggtgcttgt cgatctgaac gatcaagggg aacgttcatt
      481 tacgtttatg gtccgcccca gtgccgatct ttttttagag acgacagact tgccctgctg
      541 gcgacatggc gaatggttac atctctgttc aattgcgttg tctgccgagc cttcgcgtac
      601 cagcgcattt actgcgatga cggcgatccg gcatgccgga ggttttgtca gcttcgatcc
      661 taatattcgt gaagatctat ggcaagacga gcatttgctc cgcttgtgtt tgcggcaggc
      721 gctacaactg gcggatgtcg tcaagctctc ggaagaagaa tggcgactta tcagtggaaa
      781 aacacagaac gatcaggata tatgcgccct ggcaaaagag tatgagatcg ccatgctgtt
      841 ggtgactaaa ggtgcagaag gggtggtggt ctgttatcga ggacaagttc accattttgc
      901 tggaatgtct gtgaattgtg tcgatagcac gggggcggga gatgcgttcg ttgccgggtt
      961 actcacaggt ctgtcctcta cgggattatc tacagatgag agagaaatgc gacgaattat
     1021 cgatctcgct caacgttgcg gagcgcttgc agtaacggcg aaaggggcaa tgacagcgct
     1081 gccatgtcga caagaactgg aatagtgaga agtaaacggc gaagtcgctc ttatctctaa
     1141 ataggacgtg aattttttaa cgacaggtag gtaattatgg cactgaatat tccattcaga
     1201 aatgcgtact atcgttttgc atccagttac tcatttctct tttttatttc ctggtcgctg
     1261 tggtggtcgt tatacgctat ttggctgaaa ggacatctag ggttgacagg gacggaatta
     1321 ggtacacttt attcggtcaa ccagtttacc agcattctat ttatgatgtt ctacggcatc
     1381 gttcaggata aactcggtct gaagaaaccg ctcatctggt gtatgagttt catcctggtc
     1441 ttgaccggac cgtttatgat ttacgtttat gaaccgttac tgcaaagcaa tttttctgta
     1501 ggtctaattc tgggggcgct cttttttggc ctggggtatc tggcgggatg cggtttgctt
     1561 gacagcttca ccgaaaaaat ggcgcgaaat tttcatttcg aatatggaac agcgcgcgcc
     1621 tggggatctt ttggctatgc tattggcgcg ttctttgccg gcatattttt tagtatcagt
     1681 ccccatatca acttctggtt ggtctcgcta tttggcgctg tatttatgat gatcaacatg
     1741 tgttttaaag ataaggatca ccagtgcgta gcggcggatg cgggaggggt aaaaaaagag
     1801 gattttatcg cagttttcaa ggatcgaaac ttctgggttt tcgtcatatt tattgtgggg
     1861 acgtggtctt tctataacat ttttgatcaa caactttttc ctgtctttta tgcaggttta
     1921 ttcgaatcac acgatgtagg aacgcgcctg tatggttatc tcaactcatt ccaggtggta
     1981 ctcgaagcgc tatgcatggc gattattcct ttctttgtga atcgggtagg gccaaaaaat
     2041 gcattactta tcggtgttgt gattatggcg ttgcgtatcc tttcctgcgc gctgttcgtt
     2101 aacccctgga ttatttcatt agtgaagctg ttacatgcta ttgaggttcc actttgtgtc
     2161 atatccgtct tcaaatacag cgtggcaaat tttgataagc gcctgtcgtc gacgatcttt
     2221 ctgattggtt ttcaaattgc cagttcgctt gggattgtgc tgctttcaac gccgactggg
     2281 atactctttg accacgcagg ctaccagaca gttttcttcg caatttcggg tattgtctgc
     2341 ctgatgttgc tatttggcat tttcttcctg agtaaaaaac gcgagcaaat agttatggaa
     2401 acgcctgtac cttcagcaat atagacgtaa acttttccgg ttgttgtcga tatctcccta
     2461 tccctcaacc ggaaaataat aatactaaag tgcttagccc tgctaataat cacctaatcc
     2521 aaacgcctca ttcatgttct ggtacagtcg ctcaaatgta cttcagatgc gcggttcgct
     2581 gatttccagg acattgtcgt cattcagcga cctgtcccgt gtatcacggt cctgcgaatt
     2641 catcaaggaa tgcattgcgg agtgaagtat cgagtcacgc catatttcgc tatcaggatt
     2701 ctgtgtgatg gttacatcgc ccggcccagg gctgtttagt catcagcgct ttctgacagt
     2761 gctgagattt caacctgttg cagtaaaaat gagtagatat aaggcaagtg tgctgccaaa
     2821 cctatctttt acggggtgaa ggtagatttc gtttgaaggg tatctggtgt cccctgcagg
     2881 aatgaaattt tttatttaat ttattgttat ttatgttgtt tgttagcgtt gttaatttct
     2941 tgggccccag aaagggcccc atcattaaaa gtaatgagtt agttgatgaa cagaagcttt
     3001 cggtaattga tttgagaatc gggtagcata aacagccata taaacgaaac tacaactgcc
     3061 gagagtatca gcttcacagg tcagcccttt tgcatacagc gtgatggtat gctgatggcg
     3121 gggattcagt tcaccgctgg tgagcatgag ttccagttgt ttcatcagca gtggaaaggc
     3181 ctggtccaga tggtatgcat ccgcatcgct gaaccggcct ctgataccgg cgcggtcggc
     3241 aaggtaatgc aaccggttac cctcctgcac cagacgtgcc ccgaaacagg gcgtcacggt
     3301 gcagggcagc ccccaccagg ggcggtcgtg attgtcgtcg ggaagtgttg tcccgtggag
     3361 tgtgtctgac acgataaaat ccttacagaa aatcggctaa gaatggtccg gtagtggcga
     3421 taattctgct catcagaatt cccactcagt tcagggtgac gctcatcagc cggacatacg
     3481 gaccaaaact gtccttacgg cgttcagcaa acacggccag cacaccggga atatcctgca
     3541 cttcacgacc ggtatacgct tcagcactgc cgtgccagcg gtatttaccg gtacagaacg
     3601 gaaaaagacg ggatgtcgga tgttgttggt gaatacgcat ggcttcacca cgggtgataa
     3661 ttttcataat gggatacctc tgaagacaga agataaaagt gaaaacaggt gtgatgtggt
     3721 tgtgacgggt taaagcagac catgttcggc aaaggagaaa acctggttgc caccgactat
     3781 cagatggtcc ggcacccgga tatccaccag gcccagtgcc tgtaccagac gttcggtgat
     3841 aagccggtct gccttactgg gtgtgacttc accggacggg tgattgtgtg ccagtaccac
     3901 ggcagcggca ttgtggtaca gggcgcgttt aatcacttcc cggggatgga cttccgtgcg
     3961 gttgatggtg ccggtgaaga gggtttcacc ggcaatcagc tgattctggt tgttcagata
     4021 cagcacccgg aattcttcac gctccagtcc cgccatgttc agaatcagcc attcccgtgc
     4081 tgcacgg
//
LOCUS       DAZGLJ010000308         4063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000308 DAZGLJ010000000
VERSION     DAZGLJ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.309"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..435
                     /gene="cesT"
                     /locus_tag="ACHK49_004674"
     CDS             <1..435
                     /gene="cesT"
                     /locus_tag="ACHK49_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HFO9265161.1"
                     /translation="AEKIGIGSISFNENRLCSFAIDEIYYISLSDANDEYMMIYGVCG
                     KFPTDNPNFALEILNANLWFAENGGPYLCYESGAQSLLLALRFPLDDATPEKLENEIE
                     VVVKSMENLYLVLHNQGITLENEHMKIEEISSSDNKHYYAGR"
     gene            496..3315
                     /gene="eae"
                     /locus_tag="ACHK49_004675"
     CDS             496..3315
                     /gene="eae"
                     /locus_tag="ACHK49_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type beta"
                     /protein_id="HFO9265162.1"
                     /translation="MITHGFYARTRHKHKLKKTFIMLSAGLGLFFYVNQNSFANGENY
                     FKLSSDSKLLTQNAAQDRLFYTLKTGETVANISKSQGISLSVIWSLNKHLYSSESEMM
                     KAGPGQQIILPLKKLSVEYSALPVLGSAPVVAAGGVAGHTNKMTKMSPDATKSNTTDD
                     KALNYAAQQAASLGSQLQSRSLNGDYAKDTALGMASSQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSENMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHGGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLPNGQVVDQVGVTDFTAD
                     KTSAKADGIEAITYTATVKKNGVAQANVPVTFSIVSGTATLGANSARTDGNGKATVTL
                     KSATPGQVVVSAKTAEMTSPLNASAVIFVDQTKASITEIKADKTTAKADGSDAITYTV
                     RVMKEGAPVVDQKVTFSKDFGTLNKTEATTDQNGYATVKLSSNTPGKAIVSAKVSGVG
                     TEVKATTVEFFAPLSIDGDKVTVIGTGITGALPKNWLQYGQVKLQATGGNGKYTWKSS
                     NTKIASVDNSGVITLNEKGSATITVVSGDNQSATYTINAPGSIVIAVDKNTRVTYFDA
                     ENKCKTNSANLAQSKELLANIYSTWGAANKYPYYSGSKSLTAWIKQSSSEQSSGVSST
                     YDLVTKNQLINVGVNNKNAFSVCVK"
     gene            complement(3530..>4063)
                     /gene="escD"
                     /locus_tag="ACHK49_004676"
     CDS             complement(3530..>4063)
                     /gene="escD"
                     /locus_tag="ACHK49_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFO9265163.1"
                     /translation="IITKGNKPGFFLLSGYIPPSPKWSEVENLLLNTPGVAGWEIHNN
                     SNNKINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKILAIINAMNKNSNVK
                     ILFQNIQPYISADIFPGKILRISGTMKNPTIALDNGTSLGIGSILKGGYVIDAIDPKD
                     GINISRPDEYIHIPLSY"
BASE COUNT         1201 a          754 c          896 g         1212 t
ORIGIN      
        1 gcagaaaaaa ttggtattgg atctatttca tttaatgaaa atagattgtg ttcttttgct
       61 attgatgaga tttattatat ttcgttatct gatgctaatg acgaatatat gatgatctat
      121 ggcgtctgtg ggaaattccc gacagataat cctaacttcg ctcttgagat tttgaatgca
      181 aacttatggt ttgcagagaa tggtggacca tacctgtgtt atgaatcagg agcacaatcg
      241 ctgttgttag cgttacgttt tccgctcgat gatgctacac ctgaaaaact tgagaatgaa
      301 atagaagtcg tcgttaagtc aatggaaaac ctgtatttgg tattacataa tcagggaata
      361 acattagaaa acgaacatat gaaaatagag gaaattagtt caagcgataa taaacattat
      421 tacgccggaa gataaaagat tatttattaa tataatttac tattcattct aactcattgt
      481 ggtggagccc ataacatgat tactcatggt ttttatgccc ggacccggca caagcataag
      541 ctaaaaaaaa catttattat gcttagtgct ggtttaggat tgttttttta tgttaaccag
      601 aattcatttg caaatggtga aaattatttt aaattgagtt cagattcaaa actgttaact
      661 caaaatgccg ctcaggatcg ccttttttat acgttaaaaa caggtgaaac tgttgccaat
      721 atttctaaat cacagggtat cagtttatcg gtaatttggt cactgaataa acatttatac
      781 agttccgaaa gcgaaatgat gaaggctgga cctggtcagc agatcatttt gccactcaaa
      841 aaactgtctg ttgaatatag tgccttacct gtcttaggtt cggcacctgt tgttgctgca
      901 ggtggtgtcg ctggtcatac gaataaaatg actaaaatgt ccccggacgc gactaaaagc
      961 aacacgaccg atgacaaggc tctaaattat gcggcacaac aggccgcgag ccttggtagc
     1021 cagctccagt cgcgctcact gaacggcgat tacgcgaaag ataccgctct tggtatggcc
     1081 agcagccagg cttcgtcaca gttgcaggcc tggttacaac attatggaac ggcagaggtt
     1141 aatctgcaga gtggtaataa ctttgacggt agttcactgg acttcttatt accgttctat
     1201 gattccgaaa acatgctggc atttggtcag gtcggtgcgc gttacattga ctcccgcttt
     1261 acggcaaatt taggtgctgg ccagcgtttt ttccttcctg aaaatatgtt gggctataac
     1321 gtcttcattg atcaggattt ttctggtgat aatacccgtt taggtattgg tggcgaatac
     1381 tggcgagact atttcaaaag tagcgttaac ggctatttcc gcatgagcgg ctggcatgag
     1441 tcatacaata agaaagacta tgatgagcgc ccggcaaatg gttttgatat ccgctttaat
     1501 ggctatttac catcatatcc ggcattaggc gccaaactga tgtacgaaca gtattatggt
     1561 gataatgttg ctttgtttaa ttccgataag ttgcagtcga atcctggcgc ggcgaccgtt
     1621 ggtgtaaact acactccgat tcctctggtg acgatgggga tcgattaccg tcatggtacg
     1681 ggtaatgaaa atgatctcct ttactcaatg cagttccgtt atcagtttga taaaccgtgg
     1741 tctcagcaaa tcgagccaca gtatgttaac gagttaagaa cattatcggg cagccgttac
     1801 gatctggttc agcgtaataa caatattatt ctggagtaca aaaagcagga tattctttct
     1861 ctgaatattc cgcatgatat taatggtact gaacacagta cgcagaagat tcaattgatc
     1921 gttaagagca aatacggtct ggatcgtatc gtctgggatg atagcgcatt acgcagtcag
     1981 ggcggtcaga ttcagcatgg cggaagccaa agcgcacaag actaccaggc tattttgcct
     2041 gcttatgtgc aaggcggcag caatatttat aaagtgaccg ctcgcgccta tgaccgaaat
     2101 ggtaatagtt ctaataatgt acagctcact attaccgttt taccgaatgg gcaggttgtg
     2161 gaccaggttg gggtaacgga ctttacggct gataaaacat cggctaaagc ggatggcata
     2221 gaagctatta cctataccgc gacggttaaa aagaatggtg tagctcaggc taatgtccct
     2281 gtaacattta gtattgtatc cgggactgca actcttgggg caaatagtgc cagaacggat
     2341 ggtaacggta aggcgaccgt aacgctgaag tcggctacgc caggacaggt cgtcgtgtct
     2401 gctaaaaccg cggagatgac ttcgccactt aatgccagcg cggttatatt tgttgatcaa
     2461 accaaggcca gtattactga gattaaggct gataaaacaa cagcgaaggc agatggttct
     2521 gatgcgatta cctatactgt cagagtgatg aaggaggggg cacccgtagt agatcagaaa
     2581 gtgacctttt ctaaggattt tgggaccctg aataagactg aagcaacaac cgatcagaat
     2641 ggttatgcta ctgtaaaatt atcatccaat actcctggca aggccattgt tagtgcaaaa
     2701 gtgagtggag taggtacaga agttaaggct actaccgttg agttttttgc cccgttgagt
     2761 attgatggtg ataaagtgac cgtaattggt actggtatca cgggggctct gccaaagaac
     2821 tggttacagt atggtcaggt taagctacag gcaacagggg gcaatggaaa atacacatgg
     2881 aaatccagta atactaaaat tgcttctgtt gataactcgg gagtgataac cttaaatgaa
     2941 aaagggagtg ccacaattac tgtagtatct ggtgataatc agagtgcgac atacacaatt
     3001 aatgcaccgg gtagtattgt aattgctgtg gataaaaata ctcgagttac gtattttgat
     3061 gccgaaaaca aatgtaagac aaatagcgca aatttagcac agtcaaaaga actattggcc
     3121 aatatctatt caacatgggg tgctgcaaat aaatatcctt actattctgg ttctaaatca
     3181 ttgactgctt ggattaaaca atcctcttct gaacagtcat caggtgtatc aagcacatat
     3241 gatttggtta cgaagaacca gttgatcaat gttggagtaa acaataagaa tgctttttct
     3301 gtttgtgtaa aataatttaa ttacatctca aatcccggaa aataaaattc attttccggg
     3361 attttttatt gtacctactt gaatctttta ataaacgttt catgttaaaa tcaatgtctc
     3421 atttcggaat aagtttttcg tgtatcttgt ttgatagaga ccttgtttac catatctaca
     3481 acatctgcac aataaaaaac cctccgaaga gggggaagat aataataagt taataagaca
     3541 acggaatatg tatatattcg tctgggcggg agatgttgat accatcttta ggatcaatcg
     3601 cgtctatgac atagcctcct ttcagaatgc taccaattcc caggcttgta ccgttatcaa
     3661 gagcaattgt tggattcttc atcgtgccgc tgattctgag tatttttccg gggaaaatat
     3721 ctgctgaaat gtacggctga atgttctgaa atagtatctt gacgttacta tttttgttca
     3781 tcgcatttat aatggcaagg atcttactct cgttctgttg agaaacctcc cccgcaacaa
     3841 tgatgacatc attctttttg aaaatattga catagtttat taatttattc tttttgaact
     3901 ctgatgccag ttcattgatt ttattgttac tgttgttatg tatttcccat ccagcaaccc
     3961 ctggcgtatt cagtaacaga ttttcaactt cgctccattt tggtgaaggg ggaatgtatc
     4021 ccgataacag gaagaatcca ggcttattac cttttgtaat gat
//
LOCUS       DAZGLJ010000309         4059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000309 DAZGLJ010000000
VERSION     DAZGLJ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.310"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(34..1029)
                     /locus_tag="ACHK49_004677"
     CDS             complement(34..1029)
                     /locus_tag="ACHK49_004677"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFO9265164.1"
                     /translation="MSYSEIVKKYENISKSTNYSIDILDKLEDDLQKIFSEKNLEFTM
                     VTTGSFGRLEASEESDIDLFIFCKKESEKHYILYKKEEIEACIKKYIHKKAGDTGTFG
                     VDAVDVFDNILKNIGGGDDTNKSLTRRMLFLLEGKAIYNKNIFNSYKKKLITKYLNSS
                     SEGKLDKYLLNDVIRYYRTITTDFQHKVDADGKSWGIRNIKLRYSRKLLYFAGVMAIA
                     HASETQQDITKRTQEISNSLDIPPLERIYNISHGKAKPELIEDIFCQYEFFLSCISDP
                     EKRASLNDIKDKSDRLNSPLYNQLSEKSTEFTDNLYKLLNNAFEKNNQIHTSLIF"
     gene            complement(1058..2086)
                     /locus_tag="ACHK49_004678"
     CDS             complement(1058..2086)
                     /locus_tag="ACHK49_004678"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFO9265165.1"
                     /translation="MGDFFKRNDIDEEIEKESIGYWIQDLQKSSKLLMIIKTAAFNRL
                     YGISFLGAIDYSDQNELTKKERNRAIHSLYVAGIANYVATKRNYNSDLKEHLVAAALL
                     HDIGHMPLSHSAEPFIKEKFGYGHHELGNNLISGTCWVDSGLSKALNKNFDVSFIKGL
                     LNFELKTDGHDIFSNKINADTIDGIVRCIEYKGKNKANHLNRISIAKAAFLDNIDMTE
                     NKRLQILDNFWNTKHFVYQNFINTKHSVISDKISQIFFMENKKIAYKNLLSDERRWRE
                     QYKLLFKWLSNLKSNSIPHCLKNYEMIYTTRNYKVLKTEKDIKKRYTNNKNKAKISED
                     SLLKYREE"
     gene            complement(3076..3270)
                     /locus_tag="ACHK49_004679"
     CDS             complement(3076..3270)
                     /locus_tag="ACHK49_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFO9265166.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHEL
                     AEELQNSIQVRFESESETGT"
     gene            complement(3879..>4059)
                     /locus_tag="ACHK49_004680"
     CDS             complement(3879..>4059)
                     /locus_tag="ACHK49_004680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265167.1"
                     /translation="HLNPQHKPIYHNISLYAPQKRAERDSDAGTAKRDVLPSENRKNA
                     SGSFSAHCIAMHENE"
BASE COUNT          981 a          835 c          833 g         1410 t
ORIGIN      
        1 aaaaaaaaag gccgcttgaa agcggccttt aagttaaaaa attaatgagg tatgtatttg
       61 attgttcttt tcaaacgcat tattaagcaa tttatataaa ttatctgtaa attctgtgct
      121 tttttcgctc agttgattgt aaagagggga gtttaatcta tctgatttat cttttatgtc
      181 gtttaaacta gctcttttct ctgggtcact gatgcagctt aagaagaact catattgaca
      241 gaagatgtct tctatcagtt caggttttgc tttgccgtgg ctgatattat atattctctc
      301 taaaggtggt atatctaaac tattagatat ttcttgtgtt cgttttgtta tgtcttgttg
      361 tgtttcactg gcatgggcga tagccattac gcctgcaaaa taaagcagtt tacgggaata
      421 gcgtagtttt atatttcgga taccccaaga cttgccatcg gcatcaactt tatgctgaaa
      481 gtcagtagtg atagtcctgt aatatcttat aacatcgtta agcaaatatt tgtcgagctt
      541 tccttcgctt gaggagttca aatatttggt aattaatttt ttcttgtaac tattgaaaat
      601 gtttttgtta tatattgctt tcccttctag tagaaacaac atccttctgg ttagagattt
      661 gtttgtatca tccccaccac caatgttttt taaaatatta tcaaatacat ctaccgcatc
      721 aacgccaaac gtacctgtat caccggcttt tttatgaatg tattttttta tgcaagcctc
      781 tatttcttct tttttatata gaatgtagtg tttttccgat tcctttttac agaaaataaa
      841 gagatctata tcagattctt ctgacgcttc tagtctaccg aaagatcccg tggtgaccat
      901 ggtgaactct aaattttttt ctgaaaatat cttttgtaga tcatcctcta gtttgtcaag
      961 aatgtcaatg ctataatttg ttgattttga aatgttttcg tattttttga ctatttcact
     1021 atagctcatt ttttttatcc ctacactctt actggtatca ttcttctctg tattttaaaa
     1081 gactgtcttc acttatcttt gctttatttt tgttgtttgt atatcttttt ttaatgtctt
     1141 tctctgtctt taacacttta taatttcttg ttgtgtatat catctcgtaa ttttttaggc
     1201 aatgaggtat tgagtttgat tttaggtttg ataaccattt aaacagtaat ttatattgct
     1261 ctctccagcg tctttcgtct gagagtaagt ttttgtatgc tatcttttta ttttccatga
     1321 aaaaaatctg acttatcttg tctgatatga cgctatgttt cgtattgata aaattttgat
     1381 atacaaaatg ctttgtattc caaaaattat ctaatatctg cagcctttta ttctctgtca
     1441 tatctatgtt atctaagaat gcagcttttg caatagaaat tctatttaaa tggtttgctt
     1501 tattctttcc tttgtactct atgcacctca ctatgccatc gatagtatcg gcatttattt
     1561 tatttgagaa gatgtcgtgt ccatcggttt ttagttcgaa gtttaacagt ccttttataa
     1621 agctaacgtc aaagtttttg tttagagcct tgcttaaacc gctgtctacc cagcatgtgc
     1681 cagatattaa attatttccg agctcatgat ggccataacc aaatttttct tttataaacg
     1741 gctcagcgct atgcgataac ggcatatgtc ctatgtcatg cagtaaagct gcggctacta
     1801 aatgctcttt gagatccgaa ttgtaattcc ttttagttgc tacatagttt gctatccctg
     1861 cgacatataa agagtgaatt gctctgtttc tttctttttt tgtgagttcg ttttgatcgc
     1921 tataatcgat agcacctaag aatgatattc cgtataagcg attaaatgcg gcagttttta
     1981 ttatcattaa taatttactg gacttttgta agtcttgaat ccagtatcca atgctttctt
     2041 tttcaatctc ctcatcaatg tcatttcttt tgaagaaatc acccataata tctgctctca
     2101 aatcattcac gttaaattga ctctaacact taatatgaca acttgctcac acaatccagt
     2161 tgacgattcg tgtcatgtca agatactgtt tacctcgttg aaaatgaaaa aaccgccttg
     2221 gcgggcggtt tttcaaaggt tctctagagt tggcgctctt ttgaaccgag gtaactggct
     2281 gatagaggag cgcagtcacc aaaacttgtt ctttcagttt agccttaacc ggcgcataac
     2341 atcaagacta actcctctaa atcagttacc aatggctgct gccaatggtg cttttgcatg
     2401 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2461 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacca
     2521 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2581 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2641 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2701 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcggc gcggtctttt gctctggctt
     2761 tgcctttttg ctcacatgtt ctttccggcc ttatcccctg attctgtgga taactgtgtt
     2821 accgtttttg tgtgagtcag taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca
     2881 gtgagcgagg aagcggaaaa gcgcctgaat gtgcattttc tccttacgca tctgtgcggc
     2941 atttcacacc cggcgtgggg cacttttcat acaatctgca ctgatgccgc atggttacgc
     3001 cagtatacac tccgctatcg ctacgtgact gggtcatgtc tgcgccccga cacccgccag
     3061 ggcgcgtcag cttcgttatg ttcctgtttc actttcgctc tcaaagcgaa cctggatgct
     3121 gttctggagt tcttccgcga gttcgtgcag tcgttcgcac atggccgcct gttcgtcggc
     3181 atccagagca tccagttttt caagcagtgt caggctctgg cttttgataa atcgcgccat
     3241 attcagcgca gtttgttgct gtttgttcac cttccgttgt tctccgtcag taccgggtgt
     3301 cgggtggacc ctgaccaggt ggtaattgta agacgttgcg ggcgacggta ttacaattgc
     3361 acatcctgtc ctgtttcttt aggacgtatc atggcacagc atatcaactg ataaataatc
     3421 cctgtgcagt gcagccgctg ttgcataaaa tttcgctctg cgtcgagtgt ttcttaatca
     3481 ataattccgt tgatactccg gaaaaacgcg agtctgttct catgcctgaa atgcccacac
     3541 cccacgcaaa aacaagtttt tgctgatttt cctttataaa tagagagtta tgacaaatta
     3601 gtttctcttg ctctctttgc gatatttaaa aaagcggtgt cggcgcggtg ttgtagctgc
     3661 actgacaccg ctttgtaggg gtggtactga ctatttttat aaaaaacatc attttatatc
     3721 aggggtgctg ctggcggcgc ggtgtgtttt tttacaggac gcccctgggg gcgctgctag
     3781 gggtgtctgt tcagatatgt agcaatgagt cagggattca cgaactgaaa atgtccgaaa
     3841 atgtgtctca tgggcaccct atgcgcccaa accgttcgtc attcattttc atgcatagct
     3901 atgcagtggg ctgaaaacga tcctgacgca tttttccggt tttcactggg taaaacatct
     3961 ctttttgcag tgcctgcgtc agaatcgcgt tcagcgcgtt tctgcggtgc gtacaaactg
     4021 atgttatggt aaattggctt gtgttgagga tttaaatgc
//
LOCUS       DAZGLJ010000310         4053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000310 DAZGLJ010000000
VERSION     DAZGLJ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.311"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            153..761
                     /locus_tag="ACHK49_004681"
     CDS             153..761
                     /locus_tag="ACHK49_004681"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO9265168.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            859..2250
                     /gene="selA"
                     /locus_tag="ACHK49_004682"
     CDS             859..2250
                     /gene="selA"
                     /locus_tag="ACHK49_004682"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO9265169.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYCQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2247..>4053
                     /gene="selB"
                     /locus_tag="ACHK49_004683"
     CDS             2247..>4053
                     /gene="selB"
                     /locus_tag="ACHK49_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO9265170.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVVLTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGND"
BASE COUNT          908 a         1025 c         1202 g          918 t
ORIGIN      
        1 gagatatcca tcagatagaa acaattagga aaatatttat atcaccatga taaatatgtg
       61 atttatttgg aagtaacaaa ccatcaacat tttctgccct ttttcccatc accaggccat
      121 actgatattt ttcctcgcac aaaggactcg gcatgaaact cgtcggtagc tacaccagcc
      181 cgtttgtacg caaactttct attctgttgt tagaaaaggg cataactttc gaatttatta
      241 atgaactgcc ctataacgcg gacaacggcg tggcgcaatt taacccgtta ggaaaagtgc
      301 cggtgctggt gaccgaagag ggcgaatgtt ggtttgattc gccgatcatc gctgaatata
      361 ttgaattaat gaatgtcgct ccggcgatgt tgccgcgcga tccgctggag tcgttgcggg
      421 tgcgcaaaat tgaggcactg gcggatggca ttatggatgc cgggctggta tcggtgcgtg
      481 aacaggcgcg tccagcggcg cagcagtctg aagatgaatt gttacgccag cgggagaaaa
      541 tcaaccgcag tctggatgtg ctggaaggat atctggtcga tggcacactc aaaaccgata
      601 cggtcaatct ggcgactatc gccattgcct gtgccgtcgg atatctcaat ttccgccgcg
      661 ttgcgccagg ctggtgtgtc gatcgcccac acttagtcaa actggtcgaa aacctgttta
      721 gccgcgaaag ttttgctcgc accgaaccgc caaaggcttg atgcgcgata tgtcctcctg
      781 acccatctca cgttacaatc cgtggttatg ttaaacgccc ttctccgtgt gagagggcct
      841 tgatcagcca ggtttcctat gacaaccgaa acgcgttccc tctatagtca acttccggct
      901 attgatcgct tattgcgcga tagctccttc ctttctttgc gtgatactta tggtcacacc
      961 cgcgtggtgg aattgttgcg tcagatgctc gacgaagcgc gagaagtgat tcgtggcagc
     1021 cagacgctgc ctgcgtggtg tgaaaactgg gcgcaagaag tcgatgcccg gttgacgaaa
     1081 gaagcgcaga gcgcgctgcg tccggtgatc aacctgacgg gaaccgtgct gcataccaac
     1141 cttgggcgag ctttacaggc ggaagccgcg gtggaagccg ttgcgcaggc tatgcgttcg
     1201 ccagtgaccc tcgagtacga tctggacgac gctggacgcg gacatcgcga tcgtgcgctg
     1261 gcgcagttgc tgtgccgcat tacgggggcg gaagatgcct gtatcgttaa taacaatgcg
     1321 gcggcggtgt tattgatgtt ggcggccact gccagcggga aagaggtggt ggtttctcgc
     1381 ggcgaactgg tggagattgg cggcgcgttt cgtattcccg atgttatgcg tcaggcaggc
     1441 tgcaccctac acgaagtagg gaccaccaac cgcacgcacg cgaatgatta ttgtcaggcg
     1501 gtgaatgaaa ataccgcact gttgatgaaa gtacatacca gtaactacag cattcagggg
     1561 ttcaccaaag cgatagatga agcggaactg gtggcgctcg gcaaagagct ggatgttccc
     1621 gtagtgactg atttaggcag tggctcgctg gtcgatctta gccagtacgg tttgccgaaa
     1681 gagccaatgc cgcaggagtt gattgcggcg ggcgtcagtc tggtgagttt ctccggcgac
     1741 aagttgttag gcgggccgca ggcaggaatt attgttggta aaaaagagat gatcgcccgc
     1801 ctgcaaagcc acccgctgaa gcgtgcatta cgcgcggata aaatgaccct cgcggcgctg
     1861 gaagccacgt tgcgtcttta tttacaccct gaagctctga gtgaaaaatt accgaccctg
     1921 cgcctgctta cccgcagcgc agaggtcatt caaatccagg cacaacgttt acaggccccc
     1981 cttgccgcac attacggcgc ggagtttgcg gtacaggtta tgccatgtct ttcgcagatt
     2041 ggcagtggtt cgctgccggt tgatcgcctg ccgagcgcgg cattaacgtt tacaccccat
     2101 gatggacgcg gtagccacct tgagtcatta gccgcccgct ggcgtgaatt gccagtgccg
     2161 gtgattggtc gtatttatga cggacgattg tggctggatt tacgctgcct tgaagatgag
     2221 caacggtttt tggagatgtt gttgaaatga ttattgcgac tgccggacac gttgaccacg
     2281 gcaaaacaac cttattgcag gcgattactg gcgtaaatgc tgaccgtctg ccggaagaaa
     2341 aaaagcgcgg catgaccatc gatctcggct atgcctactg gccgcagccg gatggtcgcg
     2401 tgcctggttt tatcgacgtt cccggtcatg aaaaatttct ttccaacatg ctggcgggcg
     2461 ttggtggtat cgatcacgcg ctgttggtgg tggcgtgcga tgacggtgtg atggcacaga
     2521 cccgtgagca tctggcgatt ttgcagctga ccggtaaccc gatgctgaca gtggtgctga
     2581 ccaaagccga tcgcgtggac gaagcgcgtg ttgatgaggt tgaacgtcag gtaaaggagg
     2641 tgctgcggga atacggtttt gctgaggcaa aactgtttat caccgcagca accgaaggtc
     2701 gaggaattga tgccctgcgc gagcatctgc ttcagttacc agaacgtgaa cacgccagcc
     2761 aacatagttt ccgcctcgcg atcgaccgcg catttaccgt aaaaggtgcc gggctggtcg
     2821 tcaccggtac ggcgttaagc ggggaagtga aggtaggcga ttcactatgg ctaactggtg
     2881 taaataaacc gatgcgtgta cgtgcgctgc atgcacaaaa ccagccaaca gaaaccgccc
     2941 atgccgggca gcgtatcgcg cttaacatcg cgggtgatgc ggaaaaagag cagattaacc
     3001 gtggcgactg gctgcttgcc gatgcgccgc cagagccgtt cacacgggtg attgtcgagc
     3061 ttcaaaccca tacgccgctg acccagtggc agccgctgca tattcaccac gccgccagcc
     3121 acgttacggg acgcgtttcg ctgctggaag ataaccttgc cgagctggtg ttcgacacgc
     3181 cgctgtggct ggcggataac gatcgcttag tgctgcgcga tatctcggcc cgcaacacgc
     3241 tggcgggggc gcgtgtggtg atgcttaacc cgccgcgtcg cggtaaacgt aagccggaat
     3301 atctgcaatg gctggcgtct cttgcacggg cgcagagcga tgccgatgcg ttatctgttc
     3361 atctggaacg cggcgcggtt aaccttgcgg atttcgcctg ggcgcgccag ctcaacggcg
     3421 aagggatgcg cgaattgctg caacagcctg gttatattca ggctggttat agcttgttga
     3481 atgcgccggt tgccgcccgc tggcagcgga aaattctcga cacattagcg acttatcatg
     3541 agcaacatcg cgatgaacct ggtcctgggc gcgaacgtct gcgacgtatg gcgttgccaa
     3601 tggaagatga agcgctggta ctgttgctga ttgaaaagat gcgcgaaagc ggcgacatcc
     3661 tcagtcatca cggttggctg catctgccgg atcacaaagc gggcttcagc gaagagcagc
     3721 aggccatctg gcaaaaagca gagccgctgt ttggtgacga gccgtggtgg gtgcgtgatc
     3781 tggcaaaaga gacgggtacc gacgagcagg caatgcgcct gacgctacgc caggcggcgc
     3841 agcaaggaat aattaccgcg atcgtcaaag atcgttatta ccgtaacgat cggattgtcg
     3901 aatttgccaa tatgatccgc gatctcgatc aggagtgtgg ttcaacctgc gcggcggatt
     3961 tccgcgatcg cttaggcgta ggccgaaagc tggcaattca gattctggaa tattttgacc
     4021 gcattggctt tacgcgtcgt cgtggaaatg atc
//
LOCUS       DAZGLJ010000311         4012 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000311 DAZGLJ010000000
VERSION     DAZGLJ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.312"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="ACHK49_004684"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="ACHK49_004684"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO9265171.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVSDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="ACHK49_004685"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="ACHK49_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO9265172.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="ACHK49_004686"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="ACHK49_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO9265173.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /gene="bcsR"
                     /locus_tag="ACHK49_004687"
     CDS             3807..3995
                     /gene="bcsR"
                     /locus_tag="ACHK49_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO9265174.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT         1050 a          983 c         1007 g          972 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcctggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggcta tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgcccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gttttgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcactcacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtagc gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcgga tggaagttaa gatatcccgc ccacaacagg
     1681 ccgaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gacaataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcatgcccc attgttctgg
     2101 ggcaagcaag cgcatctgca ctagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacacagcgt cagggcttgt tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggt aatagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggcacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tgttttgcca
     3181 tgcattattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggtc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaacggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg cc
//
LOCUS       DAZGLJ010000312         3963 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000312 DAZGLJ010000000
VERSION     DAZGLJ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.313"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            212..910
                     /locus_tag="ACHK49_004688"
     CDS             212..910
                     /locus_tag="ACHK49_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO9265175.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGRYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(955..1854)
                     /gene="eamA"
                     /locus_tag="ACHK49_004689"
     CDS             complement(955..1854)
                     /gene="eamA"
                     /locus_tag="ACHK49_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO9265176.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2049..3236
                     /gene="ydeE"
                     /locus_tag="ACHK49_004690"
     CDS             2049..3236
                     /gene="ydeE"
                     /locus_tag="ACHK49_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO9265177.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPVNIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFIILALVIIAAWVLMLKGIRAKPWGQP
                     ALC"
     gene            3363..3458
                     /gene="mgtS"
                     /locus_tag="ACHK49_004691"
     CDS             3363..3458
                     /gene="mgtS"
                     /locus_tag="ACHK49_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO9265178.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            3458..3562
                     /gene="mgtT"
                     /locus_tag="ACHK49_004692"
     CDS             3458..3562
                     /gene="mgtT"
                     /locus_tag="ACHK49_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO9265179.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(3677..>3963)
                     /gene="dgcZ"
                     /locus_tag="ACHK49_004693"
     CDS             complement(3677..>3963)
                     /gene="dgcZ"
                     /locus_tag="ACHK49_004693"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO9265180.1"
                     /translation="YRYGGEEFIIIVKAANDEEACRAGVRICQLVDNHAITHSEGHIN
                     ITVTAGVSRAFPEEPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV"
BASE COUNT         1064 a          946 c          854 g         1099 t
ORIGIN      
        1 atattttttt catattttac cttactggta tataacagat tacattgttt aaatatcata
       61 tcccgcagta gctatacgtc ctggtacatt gatcacagta atacagtgat aaagttgttc
      121 aacatcaggg tgattattaa acgactcggc catacgataa accaatcagg actcaactcc
      181 ttgttttcaa tggctcttaa aaggtgcaaa agtgattttt caaaagattg cccgcttact
      241 taaatccgaa atcaatggca attcatggca tgttggtgat ttgttgccgt cagaagcgga
      301 actcgctgtt cgttataatg tttcgcgtaa taccctgcgt aaggcattat ccttgctgga
      361 aggcgaggga attattcaca gaaagcatgg ttcaggaaga tacattcaaa aaaagaattt
      421 tgttgcacac attgatcaca tgaacagttt cagtgaaatt gcgcataaaa gcggcaaaga
      481 ggcagggagt cagattatga aatttgaagt gcaggatgct tcacccacta ttgcgactga
      541 gctgaattta gtgactggtg agcaggttta ttacataaag agactgcgat tcattgagga
      601 taatgcagca caattagaag aaacgtggat gtcagtagca cgttttcctg atttaaccgt
      661 gtcacatatg caaaaatcca aattttcata tattgagaat gaatgcggga tcaaaatcat
      721 tggcaccttt gaaactttct cccctacttt tcctacccca gaaatcgcca gtattttacg
      781 gatcagccca cgggatccca tacttaaaat tcagacccag gctgtggaca gtaactctat
      841 tccgctggat tattcgttac tttacagcaa tattttcgag ttccaggtaa agtacttttt
      901 cccgcgataa aagcagacgc acagagtgct gacatcacat tctgtgcgta tggtttagcc
      961 tctaaccttt accgctttac gccaccgcaa gccaaataca ttgatataca gcccggtcat
     1021 aatgagcacc gcacctaaaa attgcagacc cgttaagcgt tcatccaaca atagtgccgc
     1081 acttgccagt ccgactacgg gcaccagtaa cgataacggt gcaacccgcc aggtttcata
     1141 gcgtcccagt aacgtccccc agatcccata accaacaatt gtcgccacaa acgccagata
     1201 catcagcgac aagatggtgg tcatatcaat agtaaccaga ctgtgaatca tggatgcgga
     1261 accatcgaga atcagcgagg caacaaagaa gggaatgatt gggattaaag cgctccagat
     1321 taccagcgac atcaccgccg gacgcgttga gtgcgacatg atctttttat tgaagatgtt
     1381 gccacacgcc caactaaatg ctgcagccag ggtcaacata aagccgagca tcgccacatg
     1441 ctgaccgttc agactatctt cgattaacac cagtacgcca aaaatcgcta aggcgatccc
     1501 cgccaattgt ttgccatgca gtcgctcccc gaaagtaaac gcgccaagca cgatagtaaa
     1561 aaacgcctgt gcctgtaaca ccagcgaagc cagtccagca ggcataccaa agttaatggc
     1621 gcagaacaga aaggcaaact gcgcaaaact gatggttaat ccatacccca agagcaaatt
     1681 cagtggtact ttcggtcgtg caacaaaaaa gatggccgga aaagcgacca gcataaagcg
     1741 caaaccggcc agcatcagcg gtggcatgtt atgaagcccc actttgatga ccacaaaatt
     1801 tagcccccat acgaccacta ccagtagcgc caacacccca tcttttcgcg acattctacc
     1861 gcctctgaat ttcatctttt gtaagcaatc aacttagctg aatttacttt tctttaacag
     1921 ttgattcgtt agtcgccggt tacgacggca ttaatgcgca aataagtcgc tatacttcgg
     1981 atttttgcca tgctatttct ttacatctct aaaacaaaac ataacgaaac gcactgccgg
     2041 acagacaaat gaacttatcc ctacgacgct ctaccagcgc ccttcttgcc tcgtcgttgt
     2101 tattaaccat cggacgcggc gctacgctgc catttatgac catttacttg agtcgccagt
     2161 acagcctgag tgtcgatcta atcggttatg cgatgacaat tgcgctcact attggcgtcg
     2221 tttttagcct cggttttggt atcctggcgg ataagttcga caagaaacgc tatatgttac
     2281 tggcaattac cgccttcgcc agcggtttta ttgccattcc tttagtgaat aacgtgacgc
     2341 tggtggtgct cttttttgcc cttattaact gcgcctattc cgtttttgct accgtgttga
     2401 aagcctggtt tgccgacaat ctttcgtcca ccagcaaaac gaaaatcttc tcaatcaact
     2461 acaccatgct aaacattggc tggaccatcg gtccgccgct cggcacgctg ttggtgatgc
     2521 agagcatcaa tctgcccttt tggctggcag ctatctgttc cgcgtttccc atgcttttca
     2581 ttcaaatttg ggtaaagcgc agcgagaaaa tcatcgccac ggaaacaggc agtgtctggt
     2641 cgccgaaagt tttattacaa gataaagcac tgttgtggtt tacctgctct ggttttctgg
     2701 cttcctttgt tagcggcgca tttgcttcat gcatttcaca atatgtgatg gtgattgctg
     2761 atggagattt tgccgaaaag gtggtcgcgg ttgttcttcc ggttaatgct gccatggtag
     2821 ttacgttgca atattccgtg ggccgccgcc ttaacccggt taacatccgc gcgctgatga
     2881 cagcaggcac cctctgtttc gtcatcggtc tggttggttt tattttttcc ggcaacagcc
     2941 tgctattgtg gggtatgtca gctgcggtat ttactgtcgg tgaaatcatt tatgcgccgg
     3001 gcgagtatat gttgattgac catattgcgc cgccaggaat gaaagccagc tatttttctg
     3061 cccagtcttt aggctggctt ggtgctgcaa ttaacccatt agtgagtggc gtagtgctaa
     3121 ccagcctgcc gccttcctcg ctgtttatca tcttagcgtt ggtgatcatt gctgcgtggg
     3181 tgctgatgtt aaaagggatt cgtgcaaaac cgtgggggca gcccgcgctt tgttgattta
     3241 agtcgaacac aataaaaatt taattcagcc ttcgtttagg ttacctctgc taatatcttt
     3301 ctcattgaga tgaaaattaa ggtaagcgag gaaacacacc acaccataaa cggaggcaaa
     3361 taatgctggg taatatgaat gtttttatgg ccgtactggg aataatttta ttttctggtt
     3421 ttctggccgc gtatttcagc cacaaatggg atgactaatg aacggagata atccctcacc
     3481 taaccggccc cttgttacag ttgtgtacaa ggggcctgat ttttatgacg gcgaaaaaaa
     3541 accgccagta aaccggcggt gaatgcttgc atggatagat ttgtgttttg cttttacgct
     3601 aacaggcatt ttcctgcact gataacggat cgttgacaca gtagcatcag ttttctcaat
     3661 gaatgttaaa cggagcttaa actcggttaa tcacattttg ttcgtcaata aacatgcagc
     3721 gatttcttcc ggtttgctta ccctcataca ttgcccggtc tgctcttcca atgaccacat
     3781 ccagaggctc ttcaggaaat gcgcgactca cacctgctgt cacggtaatg ttgatatgcc
     3841 cttcagaatg tgtgatggca tggttatcga ctaactggca aattctgaca cctgcacgac
     3901 atgcttcttc atcattagcc gctttgacaa taatgataaa ttcttcgccc ccgtagcgat
     3961 aaa
//
LOCUS       DAZGLJ010000313         3944 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000313 DAZGLJ010000000
VERSION     DAZGLJ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.314"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..769
                     /locus_tag="ACHK49_004694"
     CDS             <1..769
                     /locus_tag="ACHK49_004694"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO9265181.1"
                     /translation="GKSFIDPEVLRRQRAEETRVYGENACQALFQSRPEAIVRAWFIQ
                     SVTPRFKEALRWMAANRKAYHVVDEAELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQ
                     AGAQDCVLALENESNPHNLGGMMRSCAHFGVKGVVVQDAALLESGAAIRTAEGGAEHV
                     QPITGDNIVNVLDDFRQAGYTVVTTSSEQGKPLFKTSLPAKMVLVLGQEYEGLPDAAR
                     DPNDLRVKIDGTGNVAGLNISVATGVLLGEWWRQNKA"
     gene            complement(817..1506)
                     /gene="ung"
                     /locus_tag="ACHK49_004695"
     CDS             complement(817..1506)
                     /gene="ung"
                     /locus_tag="ACHK49_004695"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO9265182.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            1811..2194
                     /gene="grcA"
                     /locus_tag="ACHK49_004696"
     CDS             1811..2194
                     /gene="grcA"
                     /locus_tag="ACHK49_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO9265183.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(2250..2837)
                     /gene="eamB"
                     /locus_tag="ACHK49_004697"
     CDS             complement(2250..2837)
                     /gene="eamB"
                     /locus_tag="ACHK49_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO9265184.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            2940..3821
                     /locus_tag="ACHK49_004698"
     CDS             2940..3821
                     /locus_tag="ACHK49_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9265185.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNASSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
BASE COUNT         1037 a          937 c         1027 g          943 t
ORIGIN      
        1 tggtaaaagt tttattgatc cggaagtgtt gcgtcgtcag cgtgcggaag aaacccgcgt
       61 ctacggcgaa aatgcatgtc aggcactttt ccagagccgt ccggaagcga ttgttcgcgc
      121 ctggtttatc cagagtgtaa cgccgcgttt taaagaagcc ttgcgctgga tggcagcaaa
      181 ccgcaaagcg taccatgtgg tggatgaagc ggaattgaca aaagcgtcag gcacggaaca
      241 tcacggcggc gtttgcttct tgatcaaaaa gcgtaacggt acaaccgtgc agcagtgggt
      301 aagccaggca ggcgcgcagg attgcgttct ggcactggaa aacgaatcta acccgcataa
      361 cctgggtggc atgatgcgca gctgcgcgca cttcggcgtg aaaggtgtcg tggtgcagga
      421 tgcggcactg ctggagtcgg gggcggctat ccgtaccgca gaaggcggcg cagagcacgt
      481 tcagccgatt actggcgaca acattgttaa cgtgctggat gatttccgtc aggcaggtta
      541 caccgtagtg accacttcca gcgagcaggg taaaccgctg ttcaaaacta gtctgccagc
      601 gaaaatggta ctggtgctgg gtcaggaata tgaagggtta ccggatgccg cacgcgatcc
      661 gaacgatctg cgcgtgaaga ttgacggtac gggcaacgtt gccgggctga atatctctgt
      721 cgcaaccggc gttttgcttg gtgaatggtg gcgtcagaat aaagcctgat aaatcagccg
      781 ggtggcaact ctgccatccg gcatttcccc gcaaatttac tcactctctg ccggtaatac
      841 tggcatccag tcaatcggcg tctcgccacg ttgttccagc cactgatttg ccagcacaaa
      901 atggttgcag ccaaagaatc cacgatgcgc cgaaagcggc gacggatgcg gtgctttcag
      961 tacatgatgg cgttgcttat ctataatcgc ccctttcttt tgcgcatgtg atccccacaa
     1021 caaaaacacc acgccttcgc ggtgctggtt aatcaggctg atgactttat cagtaaacgt
     1081 ctcccaaccg aggctggcgt gggaatgcgc ctgacctgcg cgaaccgtca acacagtatt
     1141 gagcagcaga acgccctgac gcgcccagct ttcgagatag ccatgattag ggcgggtgaa
     1201 gcccggaata gtattttcca gctctttata catattcaat aatgacggag gaatggcaat
     1261 gccgggacga acggaaaatg ccagaccatg cgcctgtccc ggtccgtgat aaggatcctg
     1321 gccgagaatc accactttaa cgtcacccaa ctcggtaaag cggaacgcgt taaagacatc
     1381 tttttgtggt ggatagatag tgacgccgga ctgccgctcg ctggcgacgg tctgaagggt
     1441 attaagaaaa taaggttgtt gcttctcttc agccagcacg tcatgccagg ttaattcgtt
     1501 agccatctcg ctctcctgcg aatcttcaat ccgcctagct taactgcttc tttctacaga
     1561 acaaaatcat gctcattttt tagacttaaa cgagtgcaaa aaaatgtaaa ttggtaaaat
     1621 ttcacacccg cataagttga tgtaaaacaa taaaatccat ttacgaccac aatcgatgtg
     1681 tggtttttat tgatttaaat caaagaatca agggtggttg aggagtatat atacactcaa
     1741 gcaacaatgg ttttaccaat tggccgcgac aggctgaaca aatcaaataa ttttgccggg
     1801 gaggcatcac atgattacag gtatccagat tactaaagcc gctaacgacg atctgctgaa
     1861 ctctttctgg ctgctggaca gcgaaaaagg cgaagcacgt tgcatcgttg caaaagcagg
     1921 ttatgcagaa gatgaagtgg ttgcagtaag caaactgggt gacattgaat accgtgaagt
     1981 tccagtagaa gtgaaaccag aagttcgcgt tgaaggtggt caacacctga acgttaacgt
     2041 tctgcgtcgt gaaactctgg aagatgcagt taagcatccg gaaaaatatc cgcagctgac
     2101 catccgtgta tccggttatg cagttcgctt taactctctg actccggaac agcagcgcga
     2161 cgttatcgct cgtaccttta ctgaaagcct gtaaggctta agtagcaagt tactaagcgg
     2221 aagagggcga cctcttccgc tttttttcgt taatagaaaa tgcgtaccgc gcaatagacc
     2281 agcaacaggg caagcacgat atttaactgg cgaccatact ggcgaaacaa tcgctgaaac
     2341 agatgccccg ccagcgccca gcacacattg ccaaacgtcc caatcatcgc cagcaaaacg
     2401 ctgacgccaa ctacccagct tagcgcctgt gtttgcggca gaacaaacgt cgacagtgcc
     2461 gtaacaccgt acaaaatgat tttgacgttc acaaactgca aagcaaagct ggcccaaaag
     2521 ctgattggtt ttgcctgaag tccgtcttcc tttgttgggc tggtggcgat tttccacgcc
     2581 agccagacaa tatatgccgc ccccgcccaa ctcaaaaggt gtaccgctgc cgggtcaatc
     2641 actgccagtg aaaatgaaat gcccgcacac agtaacatca caatcaaaaa tcccagactc
     2701 atccctgcca gcacgcgggt actttgacga aatccatgcg acgtagcaga gctaagggcg
     2761 agaatattgt tcggtcctgg cgtcatagcg gtaatcaggg tgtaagtcca aaaagcactt
     2821 aaaagggtcg gtgtcacttg ttatctcctc tgttttggta gacagtaccc gacacggaac
     2881 atttaaaaaa atgaatgttt ttatattcta gattcgatat tttcgatagg tttggggtta
     2941 tggatctgcg ccgttttatt acgcttaaaa ccgtggtgga agagggttcc tttttgcgag
     3001 cttcgcaaaa attgtgctgt acacaatcga cggtgacttt tcatattcag cagcttgagc
     3061 aggagttttc agtccagtta tttgagaaaa ttggccgacg gatgtgcctt acccgcgaag
     3121 gaaaaaagtt gctgccgcac atttatgaac ttacccgagt gatggatacg cttcgtgagg
     3181 ctgccaaaaa ggagtcagat ccggacgggg aactacgcgt tgtttcgggc gaaacgttgc
     3241 tctcttaccg aatgccgcag gtgttgcagc gttttcggca acgcgcacca aaagtgcgtt
     3301 tatcgttgca gtcgctgaac tgttacgtga tccgtgatgc gctactgaat gatgaggccg
     3361 atgtcggcgt cttttatcgc gtagggaatg atgatgccct gaaccgacga gagttgggtg
     3421 aacaatcgct ggtgctggtg gcttcaccgc aaattgcaga tgtcgatttt actgagccgg
     3481 gaagacataa cgccagtagc tttattatca acgaaccgca atgtgtcttc cggcagatat
     3541 ttgagagcac gctgcgtcag cggcggatca cggtggaaaa caccattgag ctgttaagca
     3601 ttgaaagtat caaacgttgt gtcgcggcga atatcggtgt cagttacttg ccccgctttg
     3661 cggtggaaaa agagcttgag agcggagaat taattgaatt gccttttggc gaacagtcgc
     3721 agaccattac ggccatgtgc gcacaccatg ctggaaaagc ggttagcccg gcgatgcaca
     3781 cttttattca gtgtgttgaa gagagttttg tggctggata aaaataatgt cgggattaaa
     3841 cccggcattt aacttgtcga caaagtgcgc tttattcatg ccggatgcgg cgtgaacgcc
     3901 ttatccggcc tacaagatcg tgcaaattca atatattgca tgag
//
LOCUS       DAZGLJ010000314         3903 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000314 DAZGLJ010000000
VERSION     DAZGLJ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.315"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            116..628
                     /gene="secM"
                     /locus_tag="ACHK49_004699"
     CDS             116..628
                     /gene="secM"
                     /locus_tag="ACHK49_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO9265186.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            690..3395
                     /gene="secA"
                     /locus_tag="ACHK49_004700"
     CDS             690..3395
                     /gene="secA"
                     /locus_tag="ACHK49_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO9265187.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTVEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            3455..3844
                     /gene="mutT"
                     /locus_tag="ACHK49_004701"
     CDS             3455..3844
                     /gene="mutT"
                     /locus_tag="ACHK49_004701"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO9265188.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
BASE COUNT         1029 a          964 c         1048 g          862 t
ORIGIN      
        1 tgttttctta agcacttttc cgcacaactt atcttcattc gtgctgtgga ctgcaggctt
       61 taatgataag atttgtgcgc taaatacgtt tgaatacgat cgggatggca ataacgtgag
      121 tggaatactg acgcgctggc gacagtttgg taaacgctac ttctggccgc atctcttatt
      181 agggatggtt gcggcgagtt taggtttgcc tgcgctcagc aacgccgccg aaccaaacgc
      241 gcccgcaaaa gcgacaaccc gcaaccacga gccttcagcc aaagttaact ttggtcaatt
      301 ggccttgctg gaagcgaaca cacgccgccc gaattcgaac tattccgttg attactggca
      361 tcaacatgcc attcgcacgg taatccgtca tctttctttc gcaatggcac cgcaaacact
      421 gcccgttgct gaagaatctt tgcctcttca ggcgcaacat cttgcattac tggatacgct
      481 cagcgcgctg ctgacccagg aaggcacgcc gtctgaaaag ggttatcgca ttgattatgc
      541 gcattttacc ccacaagcaa aattcagcac gcccgtctgg ataagccagg cgcaaggcat
      601 ccgtgctggc cctcaacgcc tcacctaaca acaataaacc tttacttcat tttattaact
      661 ccgcaacgcg gggcgtttga gattttatta tgctaatcaa attgttaact aaagttttcg
      721 gtagtcgtaa cgatcgcacc ctgcgccgga tgcgcaaagt ggtcaacatc atcaatgcca
      781 tggaaccgga gatggaaaaa ctctccgacg aagaactgaa agggaaaacc gcagagtttc
      841 gtgcacgtct ggaaaaaggc gaagtgctgg aaaatctgat cccggaagct ttcgccgtgg
      901 tacgtgaggc aagtaagcgc gtctttggta tgcgtcactt cgacgttcag ttactcggcg
      961 gtatggttct taacgaacgc tgcatcgccg aaatgcgtac cggtgaaggt aaaaccctga
     1021 ccgcaacgct gcctgcttac ctgaacgcac taaccggtaa aggcgtgcac gtagttaccg
     1081 tcaacgacta cctggcgcaa cgtgacgccg aaaacaaccg tccgctgttt gaattccttg
     1141 gcctgactgt cggtatcaac ctgccgggca tgccagcacc ggcaaagcgc gaagcttacg
     1201 cagctgacat cacttacggt acgaacaacg aatacggctt tgactacctg cgcgacaata
     1261 tggcgttcag ccctgaagaa cgtgtacagc gtaaactgca ctatgcgctg gtggacgaag
     1321 tggactccat cctgatcgat gaagcgcgta caccgctgat catttccggc ccggcagaag
     1381 acagctcgga aatgtataaa cgcgtgaata aaattattcc gcacctgatc cgtcaggaaa
     1441 aagaagactc cgaaaccttc cagggcgaag gccacttctc ggtggacgaa aaatctcgcc
     1501 aggtgaacct gaccgaacgt ggtctggtgc tgattgaaga actgctggtg aaagagggca
     1561 tcatggatga aggggagtct ctgtactctc cggccaacat catgctgatg caccacgtaa
     1621 cggcggcgct gcgcgctcat gcgctgttta cccgcgacgt cgactacatc gttaaagatg
     1681 gtgaagttat catcgttgac gaacacaccg gtcgtaccat gcagggccgt cgctggtccg
     1741 atggtctgca ccaggctgtg gaagcgaaag aaggtgtgca gatccagaac gaaaaccaaa
     1801 cgctggcttc gatcaccttc cagaactact tccgtctgta tgaaaaactg gcggggatga
     1861 ccggtactgc tgataccgaa gctttcgaat ttagctccat ctacaagctg gataccgtcg
     1921 ttgttccgac caaccgtcca atgattcgta aagatctgcc ggacctggtc tacatgactg
     1981 aagcggaaaa aattcaggcg atcattgaag atatcaaaga acgtactgcg aaaggccagc
     2041 cggtgctggt gggtactatc tccatcgaaa aatcggagct ggtgtcaaac gaactgacca
     2101 aagccggtat taagcacaac gtcctgaacg ccaaattcca cgccaacgaa gcggcgattg
     2161 ttgctcaggc aggttatccg gctgcggtga ctatcgcgac caatatggcg ggtcgtggta
     2221 cagatattgt gctcggtggt agctggcagg cagaagttgc cgcgctggaa aatccgaccg
     2281 tagagcaaat tgaaaaaatt aaagccgact ggcaggtacg tcacgatgcg gtactggaag
     2341 caggtggcct gcatatcatc ggtaccgagc gtcacgaatc ccgtcgtatc gataaccagt
     2401 tgcgcggtcg ttctggtcgt cagggggatg ctggttcttc ccgtttctac ctgtcgatgg
     2461 aagatgcgct gatgcgtatt tttgcttccg accgagtatc cggcatgatg cgtaaactgg
     2521 gtatgaagcc aggcgaagcc attgaacacc cgtgggtgac taaagcgatt gccaacgccc
     2581 agcgtaaagt tgaaagtcgt aacttcgaca ttcgtaagca actgctggaa tatgatgacg
     2641 tggctaacga tcagcgtcgc gccatttact cccagcgtaa cgaactgctg gatgtcagcg
     2701 atgtgagcga aaccatcaac agcattcgtg aagatgtgtt caaagccacc attgatgcct
     2761 acattccgcc acagtcgctg gaagaaatgt gggatattcc ggggctgcag gaacgtctga
     2821 agaacgattt cgacctcgat ttaccaattg ccgagtggct ggataaagaa ccagaactgc
     2881 atgaagagac gctgcgtgag cgcattctgg cgcagtccat cgaagtgtat cagcgtaaag
     2941 aagaagtggt tggtgctgag atgatgcgtc acttcgagaa aggcgtcatg ctgcaaacgc
     3001 ttgactccct gtggaaagag cacctggcag cgatggacta tctgcgtcag ggtatccacc
     3061 tgcgtggcta tgcacagaaa gatccgaagc aggaatacaa acgtgaatcg ttctccatgt
     3121 ttgcagcgat gctggagtcg ttaaaatatg aagttatcag cacgctgagc aaagtccagg
     3181 tacgtatgcc tgaagaggtt gaggagctgg aacaacagcg tcgtatggaa gccgagcgtt
     3241 tagcgcaaat gcagcagctt agccatcagg atgacgactc tgcagccgca gctgcactgg
     3301 cggcgcaaac cggtgagcgc aaagtaggac gtaacgatcc ttgcccgtgc ggttctggta
     3361 aaaaatacaa gcagtgccat ggtcgcctgc aataaaagct aacagttgaa gtaaaaggcg
     3421 caggattctg cgcctttttt ataggtttaa gacaatgaaa aagctgcaaa ttgcggtagg
     3481 tattattcgc aacgagaaca atgaaatctt tataacgcgt cgcgcagcag atgcgcacat
     3541 ggcgaataaa ctggagtttc ccggcggtaa aattgaaatg ggcgaaacgc cggaacaggc
     3601 ggtggtgcgt gaacttcagg aagaagtcgg gattacccca caacattttt cgctatttga
     3661 aaaactggaa tatgaattcc cggacaggca tataactctg tggttctggc tggtcgaaag
     3721 ctgggaaggg gagccgtggg gtaaagaagg gcaacccggt gagtggatgt cgctagtcgg
     3781 tcttaatgcc gatgattttc cgccagccaa tgaaccggta attgcgaagc ttaagcgtct
     3841 gtaggtcgga taaggcgttc acgccgcatc cgacatttgc acaagatgcc tgatgcgacg
     3901 ctg
//
LOCUS       DAZGLJ010000315         3747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000315 DAZGLJ010000000
VERSION     DAZGLJ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.317"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..1385
                     /gene="dcuC"
                     /locus_tag="ACHK49_004702"
     CDS             3..1385
                     /gene="dcuC"
                     /locus_tag="ACHK49_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO9265189.1"
                     /translation="MTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIM
                     GHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVV
                     KLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGA
                     AAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFWQ
                     RYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVIC
                     MLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQS
                     LISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYL
                     TIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMV
                     PGTAAAVTGK"
     gene            complement(1426..2106)
                     /gene="dpiA"
                     /locus_tag="ACHK49_004703"
                     /gene_synonym="citB"
     CDS             complement(1426..2106)
                     /gene="dpiA"
                     /locus_tag="ACHK49_004703"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO9265190.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVPEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2075..3733)
                     /gene="dpiB"
                     /locus_tag="ACHK49_004704"
     CDS             complement(2075..3733)
                     /gene="dpiB"
                     /locus_tag="ACHK49_004704"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO9265191.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          853 a          989 c          904 g         1001 t
ORIGIN      
        1 tgctgacatt cattgaactc cttattgggg ttgtggttat tgtgggtgta gctcgctaca
       61 tcattaaagg gtattccgcc actggtgtgt tatttgtcgg tggcctgtta ttgctgatta
      121 tcagtgccat tatggggcac aaagtgttac cgtccagcca ggcttcaaca ggctacagcg
      181 ccacggatat cgttgaatac gttaaaatat tactaatgag ccgcggcggc gacctcggca
      241 tgatgattat gatgctgtgt ggatttgccg cttacatgac ccatatcggc gcgaatgata
      301 tggtggtcaa gctggcgtca aaaccattgc agtatattaa ctccccttac ctgctgatga
      361 ttgccgccta ttttgtcgcc tgtctgatgt ctctggccgt ctcttccgca accggtctgg
      421 gtgttttgct gatggcaacc ctatttccgg tgatggtaaa cgttggtatc agtcgtggcg
      481 cagctgctgc catttgtgcc tccccggcgg cgattattct cgcaccgact tcaggggatg
      541 tggtgctggc ggcgcaagct tccgaaatgt cgctgattga cttcgccttc aaaacgacgc
      601 tgcctatctc aattgctgca attatcggca tggcgatcgc ccacttcttc tggcaacgtt
      661 atctggataa aaaagagcac atctctcatg aaatgttaga tgtcagtgaa atcaccacca
      721 ctgctcctgc gttttatgcc attttgccgt tcacgccgat catcggtgta ctgatttttg
      781 acggtaaatg gggtccgcaa ttacacatca tcactattct ggtgatttgt atgctgattg
      841 cctccattct ggagttcctc cgcagcttta atacccagaa agttttctct ggtctggaag
      901 tggcttatcg cggaatggca gatgcgtttg ctaacgtggt gatgctgctg gttgccgctg
      961 gggtattcgc tcaggggctt agcaccatcg gctttattca aagtctgatt tctatcgcta
     1021 cctcgtttgg ttcggcgagt atcatcctga tgctggtatt ggtgattctg accatgctgg
     1081 cggcagtcac gaccggttca ggcaatgcgc cgttttatgc gtttgttgag atgatcccga
     1141 aactggcgca ctcttccggc attaacccgg cgtatttgac tatcccgatg ctgcaggcgt
     1201 caaacctggg ccgtaccctt tcgcccgttt ctggcgtagt cgttgcggtt gccgggatgg
     1261 cgaagatctc gccgtttgaa gtcgtaaaac gcacctcggt accggtgctt gttggtttgg
     1321 tgattgttat cgttgctaca gagctgatgg tgccaggaac ggcagcagcg gtcacaggca
     1381 agtaaatagt aatgccggag gcaagtttcc tccggcttta tcatgtcacc cactgtggta
     1441 tatgcgttgc ggtctgccaa ctttgccgtg aacaatttca gcaataatca gatggcggct
     1501 ggcgcaatat tcaagataac gcctggcagt ggtgcgactg atggtcagtg cctgcgccac
     1561 cgtttctgcc gtatgttgca caccaggctc tttaaacagt tttcgcaccg cgtttagcgt
     1621 caagggatca atgccggtcg gcagttcgtc tttaggttca ccgcgagcat aggcattaaa
     1681 catctcatca atttgcttct ggctggcgct atcaatactt tccagcatat gtttacgctg
     1741 gcggaaacgg gttagcgttt gccccagccg ttcataggca atgggcttaa tgagataatc
     1801 aaatacacca caacgtactg cttcaggcac cgtttccata tcgctggctg cagtggtaaa
     1861 caccacgtcg ccgggataat gcgcctgcac cagttcatgc aataaattaa tccctctgcc
     1921 gtcaggaaga tagttatcga gcaagattag ccccggctta aaacgctcga tcatcattcg
     1981 ggcctgcgcc aggtttcccg ccagtaatat ctgactgaat ccgggaatgt gacgaatata
     2041 ttccgcatgc atctctgcca gcggcgtttc gtcctcaacg atcaataggg ttaatggagc
     2101 tgtcattagg tttcactttc ggaatataga ttgaaaacaa ggtaccgcag ggatcattat
     2161 cttcgagagt gataacacca ccgcagcgcg ttacgtagct ggcaatcaag tacaacccaa
     2221 tgccatgttc accgggctcg tcagcacgcg tactgacccc ctgctcaaat attttgtctc
     2281 gtagagactc tggaacgccg cagccctgat cggcgacttc aatcaccaca tcatcgcctt
     2341 catcgctgag gaataattca acgatcttgt ttccttcatc gctacgcagg ctggcttcga
     2401 aggcgttatc aagtaaattg cccacaatgg ctgcaaactc ggtgctatcc agtcctggcg
     2461 gcagttgcga aagctggcta ccggggacaa tgatcatttt tagccccagt tcccgggcgc
     2521 gctgcacttt accaaaaagc agccccgcca cctggcgatc ggcaaacgcc tcacgcaggc
     2581 tgtcaataag ctgttgctgg gcctgagact ccccctgcac catcgccagc acgcgatcat
     2641 actctttcat ctgcaacaga ccattgagcg tcgacatcca attgagatgc tcgtgtcgca
     2701 atgtacgaag gctctcgacg tattgtttta tttgcgtcag ttgcgcattg agggtggaaa
     2761 tttcgtcttt actacgaaag ctgataatgg cccccagcaa atcatcacct gaacgaatag
     2821 cttcccgatt ggcaataacg ctcagaccgt taaagttagc caccacatcc tgacgttttt
     2881 catcaatctg ttcggtaaag aaatcggcgg gcctgaccac ttcagcaatg ggtttaccca
     2941 accattgccg tccgggagag ctcagcccca gcatctttct tgcgttacga ttgatggcgg
     3001 taatgtaacc atgcggatcc accgcaatca gcccttcata aaccgaacta aacagcgcct
     3061 cttgctgacg gaccacgcgt gcgatttgct ttggctccat gcccatcatt tgccgacgga
     3121 tatgcgctgc caggaaccac gacagcaaca tcagaatccc taacagcacg acaaacacgc
     3181 ctgccatcgg taataaaaac tcagcccgcc agctatcgat tttactcacc aggtagccaa
     3241 tcgacaccac gccgatgact tttccatcgt catcaaagat tggcgttttg gcgcgcatcg
     3301 ccatccccat tgaccctttc ccggtaatga agtagctctc ccctttctcc agcgcgccct
     3361 gtttggtgaa ctgcatagga taaccaattt tctccggatt aggatggtaa aggcggatcg
     3421 agtgccggtc cccaatcacc acataatcaa aatcggtatc tctttgtaat ttgttagcga
     3481 tggtcgccag ccgtttgtag tcacgcgttt tcaccgcaga gatgacactg tcattggagg
     3541 caataatttt cgcctgattc attgccatgt cgcgtacatg aagcgttaaa tagtcctcaa
     3601 aactggccgt aaaatattgc gccagggctg caatgacaaa tattgagaac accagaatca
     3661 gcaaaaagat acgcagcgga aatgccagtc tttggaaaaa tgcaaactgt ttattctcgt
     3721 taagctgcaa cattgttttc cttgcgg
//
LOCUS       DAZGLJ010000316         3640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000316 DAZGLJ010000000
VERSION     DAZGLJ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.318"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..363
                     /locus_tag="ACHK49_004705"
     CDS             <1..363
                     /locus_tag="ACHK49_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO9265192.1"
                     /translation="KVPAAKITKAYFELGMTFPELYDDSHPEALARNTQKIFRWLDKD
                     TPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYREIVERRDRLVKDVDDFVASAVV
                     LYDQMNRGGPAGNAVVMH"
     gene            386..1348
                     /locus_tag="ACHK49_004706"
     CDS             386..1348
                     /locus_tag="ACHK49_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9265193.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIAPSTVDPSNFDGSTVDNKLPIRGAMIDPDPSVLK
                     PEPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            1355..2096
                     /locus_tag="ACHK49_004707"
                     /pseudo
     CDS             1355..2096
                     /locus_tag="ACHK49_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            2122..2883
                     /locus_tag="ACHK49_004708"
     CDS             2122..2883
                     /locus_tag="ACHK49_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO9265194.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGEAGVTEGQALQPEAPDVVTEEVAPKVTADMMVEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMSGGNLPADPKAAPVTKNDGKA
                     FPQPAGAALPVREAATQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRA
                     KGQVQKWERVCAALRELNKCRDILRDITATREQQR"
     gene            2916..3197
                     /locus_tag="ACHK49_004709"
     CDS             2916..3197
                     /locus_tag="ACHK49_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO9265195.1"
                     /translation="MILRQCAGTMKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            3194..3421
                     /locus_tag="ACHK49_004710"
     CDS             3194..3421
                     /locus_tag="ACHK49_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265196.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            3414..>3640
                     /locus_tag="ACHK49_004711"
     CDS             3414..>3640
                     /locus_tag="ACHK49_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265197.1"
                     /translation="MSEIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERP"
BASE COUNT          948 a          779 c         1128 g          785 t
ORIGIN      
        1 aaagtaccgg ctgcgaaaat taccaaagcg tatttcgagc tgggaatgac gttcccggaa
       61 ctgtatgacg acagccatcc ggaagccctg gcccgtaata cccagaaaat tttccgttgg
      121 ctggataaag acacccctga tgctgttgaa aaaatgcagg ctctgttacc ggcgatcgaa
      181 aaggcgatgc cgcctttgct ggtggcccgt atgcgcagcc acagttctga atattaccgt
      241 gagatcgtcg aacggaggga tcggctggtg aaggatgtcg atgattttgt tgcgtcagcg
      301 gttgttttgt atgaccagat gaatcgcggc ggcccggcag ggaatgctgt ggtgatgcac
      361 taaaagcacg gtgttcgggg gttttatgag cagcaagctt catggtcttg tctgggaagg
      421 gtgcgccttc accggcatga tcttatccag ggtggcggtt atggcccgtc ttgcagacta
      481 cagcaatgac gagggcgtgt catggcctgc cattgaaact atccggcgtc agatcggtgc
      541 aagaagtgaa tccacagtga aatcggctat tgcagaactg gcgaaagagg gctggctgac
      601 gaaggaagag cgtaaggtcg gtgggcgtaa tgtaagcaat atctatcggc ttaatgtgga
      661 aaaactcgaa gcagctgcgg cggcggcgcg tgagtcatat aaaccgaaaa gaaaaattag
      721 cccggcaaaa aatgacccgt taacagttga cccgtcaaat attgccccct caacggttga
      781 cccgtcaaat tttgatggat caactgttga taacaaactg ccgattaggg gggcgatgat
      841 tgaccccgat ccgtcagtat taaaacctga gccgtcagat aaaagatctt cttgtccgga
      901 cgcttcgcaa ccggacccgc agacggctga acaggatttt ttaacccgac accctgacgc
      961 ggttgtgttc agtgcgaaaa aacgccagtg gggaagtcag gaagatttgg tgtgcgcaca
     1021 gtggatctgg ggacgaatcg tgagtcttta cgagcaggcg gccagcgatg atggcgagat
     1081 cacgagaccg aaagaaccca actggactgc atgggccaat gacgtgcgga caatgcggat
     1141 gctggatggc agaactcaca gacaaatttg tgaaatgttt gggcgtctcc agcgggattc
     1201 gttctgggta aaaaacatca tgagtccggc aaaactccgg gaaaaatggg atgaactggt
     1261 tatccgcctg gggcgttcgc ccgcgcagcg ttgcgtgaat cacatttctg aaccggacac
     1321 tgaaataccg ccgggattca gggggtgacg tgtcatgaaa aacattgcgg cagttggggt
     1381 tcttgaacgt attcgcagac ttgcaccaca ggggggcggt tccaccgtac cggacggtgg
     1441 aggagtggcg ggaatggcaa cttgctgaag gacgaaaacg cagcgaggag attaaccgcc
     1501 tgaatcatca ggtgcgggtt gaaaaaatcc tgaaccgtgc gggcatccag ccgcttcaca
     1561 ggaagtgctc attcgggaac taccgggtgc agaacgacgg tcagcgccat gctctgagcc
     1621 aggcgaaatc cattgccgat gaattgatga ccggatgtac aaacttcgtg ttcagcggta
     1681 aacctggtac cggtaaaaat cacctggcag cagcgattgg caatcggctg atggcgaagg
     1741 ggagaagcgt gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gacggctacg
     1801 acaacggcca gtccggggaa aaatttttac aggagctttg tggagttgac cttctggtcc
     1861 ttgatgaaat tggcatgcag cgggatacgc gcaacgagca ggtcacgctg aaccagatag
     1921 tcgaccgcag aacggcttcg atgcgtagtg tcggaatgct gacgaacctg aatcacgcag
     1981 cgatgagcac actcctcgga gatcgggtga tggaccgtat gaccatgaat ggtggtcgtt
     2041 gggtgaattt taactgggag agctggcggt caaacgttgg acgtcagggt atgtgagaat
     2101 ttttgacgag gtaaattttc gatggaaact gtattgcatg cactgaaagc gatgggaaaa
     2161 gccaattctg ttgaactggc ggcgcggctt gatatcagcc gtgaagaagt tcttaacgaa
     2221 ctgtgggaac tcaaaaaaaa tggcgttgtt gataaaacgg gtcacacctg gtttctggct
     2281 gtcgaaggtg aagccggggt aaccgaaggg caggcactac aacctgaagc gccggatgtg
     2341 gtaaccgaag aggtcgctcc aaaagttacc gcagacatga tggttgagtt tatcggtcag
     2401 gatggtgcta aaacgtgtga ggaactggcg ggtaagttcg gcgtcagtac tcgcaaggtt
     2461 gcttccacgc tggcggtggt aaccgcaacg gggcggctgg cacgcgttaa tcagaacggt
     2521 aaatttcgtt actgcatgtc ggggggtaat ttaccagcag atccgaaagc cgcgccggta
     2581 acgaaaaatg atggtaaggc ctttcctcag ccagcaggtg ctgcgttacc agtccgggaa
     2641 gccgcaacac aggaagaaat aaaaacggaa agtgtggcgg tcacagtgca gtcacagccg
     2701 tcgttcacca gaaagcatcc ggatggtctg attttaccat cgctgcatgt ggctaaccgc
     2761 gagctgcgcc gggcaaaagg tcaggttcag aagtgggagc gagtctgcgc cgcgctgcgg
     2821 gagctgaaca agtgccggga tattctccgg gatattaccg ccaccagaga acagcagcgg
     2881 tgagtgggtg gaagacgtgg tgccgggcgg aaatcatgat actccggcag tgtgcgggaa
     2941 cgatgaaggt aaaaagcgtt ggcgcactta tcggacgaac tgaagcggca gtgagaacga
     3001 aggcacggga gctgggcatc agcatgatgt tacgtggtga ttttcacccg tcggcaaaat
     3061 attctcagcg tgatattgag ctggcgcggc aactgcatca gagaggcatg caaagaaggg
     3121 aaattgccag aaaattaggc atgccgctgc gcatagtgaa taactacgtt tatttcgaca
     3181 ggagggtgtc tgcgtgaaaa tcctgtatca ggattacggc ccggtggggc aggtggttat
     3241 cagcagtact gtaatggagt ttcggaagca taaccgtgtg gtggatgctg tgctgttaac
     3301 ctgtccgggg atatcggcga gtcgtgcagg tgtgtttatt atgaagacga aattatatgg
     3361 cagtaaggcg tggataaaga aggcgtatcg tgtagcgttg caggaggtta acagtgagtg
     3421 aaattaaaga aatgccggta gttcgtgacg gatatggcta ctggacacat cctgaatatg
     3481 aaaaattctg tgatggtcgg gaatatattt caacggaaga gtttaacgcc tggatggagg
     3541 aaaataatct tcaatacgtc ctctgcttca gagatgaagg atgtgctgac cttgatgcgt
     3601 gtgatgctga tatttctgca tgggaaccgg aacgaccaga
//
LOCUS       DAZGLJ010000317         3599 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000317 DAZGLJ010000000
VERSION     DAZGLJ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.319"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..810
                     /locus_tag="ACHK49_004712"
     CDS             <1..810
                     /locus_tag="ACHK49_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO9265198.1"
                     /translation="DFLNGDPDQPIIMGRTYHHENRTPGSLPGTKTQMTIRSKTYKGS
                     GFNELKFDDATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIQ
                     TVGVNQIETVGSNQIINVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQI
                     GLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKD
                     GQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKPPDEKQDTPDMREY"
     gene            878..>3599
                     /locus_tag="ACHK49_004713"
     CDS             878..>3599
                     /locus_tag="ACHK49_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO9265199.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVQPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDI
                     RLQIRDDALILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWA
                     SLPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDLLPAPLPPYRVLTGMADRFGR
                     TLAYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKPHTASLSSPDSPRPL
                     SAPSFPDTLPGTEYGADSGIRLSAVWLMHDPEYPENLPAAPLVCYDWTPRGELAAVYD
                     RSGTQMRHFTYDDKYRGRMVGHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQD
                     RITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTE
                     YGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSR
                     SGETVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQ
                     MTAVHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQY
                     DAWGKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQ
                     RYHYDLTGKLTQSEDEGLVTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLS
                     EGHRVAVHYGYDDKGRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPP
                     VEWLTYGSGYLAGMKLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQ
                     LQSQHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAP"
BASE COUNT          827 a         1001 c         1175 g          596 t
ORIGIN      
        1 gacttcctca acggcgaccc ggaccagccg attattatgg ggcgtaccta ccaccatgaa
       61 aaccgcacac ccggcagcct gccggggacg aagacgcaga tgacgatccg ctcgaaaacc
      121 tataagggca gcgggtttaa tgaactgaag tttgacgatg cgacagggaa agaacaggtc
      181 tacatccacg cgcagaagaa catgaacacc gaggtgctga ataaccgcac cacggatgtg
      241 ataaacaacc atgctgagac cattggcaac aatcagatga ttgcggttac caacaatcag
      301 atacagacgg tgggcgttaa ccagatagag acggtgggca gtaaccagat cattaacgtg
      361 ggttctgttc aggtggaaac gattggactt gttcgtgcgc tgaccgtggg cgtggcgtac
      421 cagacgacgg taggtggcat tatgaacact tcggtggctc tgatgcagtc ctcgcagatc
      481 ggtttgcata aatcgttgag ggtcgggctg ggttatgacg tcaaagtcgg aaataacgtt
      541 accttcaccg ttggtaaaac gaaaaaggat gataccgggc agaccgcgat ttactccgcc
      601 ggtgagcatc tggagctctg ctgtggtaag gcaaggctgg tgctgacgaa ggacggacaa
      661 atttttctca acggcacaaa aattcatttg cagggtaagg agcaggttaa tggtgactca
      721 ctgttgatta actggaactg tgcagcctcg aaatccccac cgaagccccc tgatgaaaag
      781 caggatacgc cggatatgag agagtactga tcccgtacct gttcggaatt cagtaacaaa
      841 ccagcattaa aaataaacag aagaaggaat ttgaatcatg agcggaaaac cagcggcgcg
      901 tcagggagat atgactcagt atggcggtcc cattgtccag ggttcggcag gtgtaagaat
      961 tggcgcgcca actggcgtgg cgtgctcggt gtgtccgggc gggatgacct cgggcaaccc
     1021 ggtgaacccg ctgctcggtg caaaagtaca gcccggcgag acggaccttg cgctgcccgg
     1081 cccgctgccg ttcattctct cccgcaccta cagcagctac cggacgaaga cgcctgcacc
     1141 ggtgggcgtt ttcggccccg gctggaaagc gccttctgat atccgcttac agatacgcga
     1201 tgatgcactg atactcaacg acaacggcgg gcggagcatt cactttgagc cgctgctgcc
     1261 gggggaggcg gtgtacagcc gcagtgagtc aatgtggctg gtgcgtggcg gtaaggcagc
     1321 gcagccggac ggccacacgc tggcacggct gtgggcgtcg ctgccaccgg atatccggtt
     1381 aagcccgcat ctttacctgg cgaccaacag cgcacagggg ccgtggtgga tactggggtg
     1441 gtctgagcgg gtgccgggtg ctgaggactt actgccagcg ccgctgccgc cgtaccgggt
     1501 gcttaccggg atggcggacc gcttcgggcg gacgctggcg taccggcgtg aggccgccgg
     1561 tgacctggcc ggggaaatca ccggcgtgac ggacggtgcc gggcgggagt tccgtctggt
     1621 actgaccaca caggcacagc gggcggaaga ggcccgtaaa ccgcacaccg cttcgttatc
     1681 ttcccctgac agtccccgcc ctctatcagc tccttccttc cccgacacac tgcccggtac
     1741 ggaatacggc gctgacagcg gtatccgtct ttcggcggtg tggctgatgc acgacccgga
     1801 atacccggaa aatttacctg ccgcgccact ggtgtgctac gactggacgc cacgcggtga
     1861 gctggcggcg gtgtatgacc gcagcggtac gcagatgcgc cactttactt acgatgataa
     1921 ataccggggc cggatggtgg ggcaccgtta tgcaggcagg ccggagatgc gctaccgcta
     1981 cgacgatgcg gggcgggtgg tggagcaact taacccggca ggcctgagtt accgctacca
     2041 gtatgagcag gaccgcatca ccgtcacgga cagcctgaac cggcgtgagg tgctgcatac
     2101 agaaggcggg gccgggctga agcgggtggt gaaaaaagaa ctggcggacg gcagcgtcac
     2161 gcacagcggg tatgacgcgg caggaaggct cacggcgcag acggacgcgg cgggacggcg
     2221 gacagagtac ggtctgaatg tggtgtccgg cgatatcacg gacatcacca cgccggacgg
     2281 gcgggagacg aaattttact ataacgacgg gaaccagctg acggcggtgg tgtccccgga
     2341 cgggctggag agccgccggg aatatgatga accgggcagg ctggtatcgg agacatcgcg
     2401 cagcggggag acagtacgct accgctacga tgacgcgcac agtgagttac cggcgacgac
     2461 aacggatgcg acgggcagca cccggcagat gacgtggagc cgctacgggc agttgctggc
     2521 gttcactgac tgctcgggct accagacccg ctatgaatac gaccgcttcg gccagatgac
     2581 ggcggttcac cgcgaggaag gtatcagcct ttaccgccac tatgacaacc gtggccggtt
     2641 aacctcggtg aaagacgcac agggccgtga aacgcagtat gagtacaacg ccgcaggcga
     2701 cctgactgcc gttatcaccc cggacggcaa ccggagcgag acacagtacg atgcgtgggg
     2761 aaaggcggtc agcaccacgc agggcgggct gacgcgcagt atggaatacg atgctgccgg
     2821 acgtgtcatc agtctgacta acgagaacgg cagtcacagc gacttcagct acgatgcgct
     2881 ggaccggttg gtacagcagg gcggctttga cgggcggacg caacgttatc attatgacct
     2941 gaccggaaaa ctcacgcaga gtgaagatga ggggcttgtc accctctggt actacgatga
     3001 atcggaccgt atcactcacc gcacggtgaa cggcgaaccg gcagagcagt ggcagtatga
     3061 tgaccacggc tggctgacag acatcagcca cctgagcgaa ggccaccgtg tcgctgtcca
     3121 ctacggctac gacgataagg gccgcctgac cggcgaacgg cagacggtgg agaacccgga
     3181 gacgggggaa ctgctgtggc agcatgagac aacacacgcc tacaacgaac aggggctggc
     3241 aaaccgcgtc acgccggaca gcctgccgcc ggtggagtgg ctgacctacg gcagcggtta
     3301 tctggcgggc atgaagcttg gcgacacgcc gctgctggag tacacgcgcg accgcatgca
     3361 ccgggagacg gtgcgcagct ttggcagcat ggcaggcagt aatgccgcat ataaactgac
     3421 cagcacatac acccccgcag gccagttaca gagccagcac ctgaacagcc tggtgtatga
     3481 ccgtgactac gggtggaatg acaacggcga cctggtgcgc atcagcggcc cgcgacagac
     3541 gcgggaatac ggctacagcg ccacgggcag gctggagagc gtgcgtaccc tcgcaccgg
//
LOCUS       DAZGLJ010000318         3519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000318 DAZGLJ010000000
VERSION     DAZGLJ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.320"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..296)
                     /locus_tag="ACHK49_004714"
     CDS             complement(<1..296)
                     /locus_tag="ACHK49_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO9265200.1"
                     /translation="MSKVFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEASASFVPVETES"
     gene            complement(392..580)
                     /locus_tag="ACHK49_004715"
     CDS             complement(392..580)
                     /locus_tag="ACHK49_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO9265201.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPLKKVSLYLNNETPAG"
     gene            complement(577..765)
                     /gene="dicB"
                     /locus_tag="ACHK49_004716"
     CDS             complement(577..765)
                     /gene="dicB"
                     /locus_tag="ACHK49_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO9265202.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            complement(1298..1672)
                     /locus_tag="ACHK49_004717"
     CDS             complement(1298..1672)
                     /locus_tag="ACHK49_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265203.1"
                     /translation="MEFKDLPMPFQEMAANVVRSQLATLDLSTVEKETIDNISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLELMDIINKGFGLLMKKKGIRIAPLENHFTASSINS
                     CDLKHHTSDGKVESNNKISINH"
     gene            complement(1695..1913)
                     /gene="ydfC"
                     /locus_tag="ACHK49_004718"
     CDS             complement(1695..1913)
                     /gene="ydfC"
                     /locus_tag="ACHK49_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO9265204.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITRLEKAAALSIARMITYPR"
     gene            complement(1943..2071)
                     /gene="ydfB"
                     /locus_tag="ACHK49_004719"
     CDS             complement(1943..2071)
                     /gene="ydfB"
                     /locus_tag="ACHK49_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO9265205.1"
                     /translation="MDFNTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSNG"
     gene            complement(2073..2228)
                     /locus_tag="ACHK49_004720"
     CDS             complement(2073..2228)
                     /locus_tag="ACHK49_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO9265206.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTRSKTFKTENGARRW
                     LERNSGE"
     gene            complement(2501..3217)
                     /locus_tag="ACHK49_004721"
     CDS             complement(2501..3217)
                     /locus_tag="ACHK49_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO9265207.1"
                     /translation="MSTIATSLGSNIGYMKNTISERIRNRRKDVGLTQQQVAKAIGIS
                     RVSVTKWENGSSKPDGENLYLLSKLLSKSPEWILYGKDGHDKTDDLRLNQYPYISDNI
                     ARLPVLTWEQAGYWDMSCPVTKIPGIKNWVDVMTKTAENSFLLHVEGDAMTNSNGLPT
                     IPDGSTVLITPCSSNIRELVGKIILIQLEGTPNVTLKKVAIDGPNIYLLSLNPLYKPI
                     ELNGGYTIKGKVSQIHQYLD"
     gene            3267..3482
                     /locus_tag="ACHK49_004722"
     CDS             3267..3482
                     /locus_tag="ACHK49_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO9265208.1"
                     /translation="MFKDDVLRYFKKKRLVAEALGISHVAVVRWKAVIPKLRAMELDE
                     ITNGELKYNPELYKKQDSTSNEGKNDS"
BASE COUNT          915 a          794 c          769 g         1041 t
ORIGIN      
        1 gattcagtct caaccgggac aaaggaagca gacgcctcat cccagcggtt ttcctgcata
       61 tattcagcat cccaggaatc gagggcaggg cggggtatgc cgggtttatc ctcacagaca
      121 ataaatttat aagcgcagtc ctgagctgca gggaattgct ccagaaattg ccaatgaaat
      181 tttgcgcgtg cgcgacgctc atcaccagct tcaatggcag tggccaccgc aacagcgcta
      241 tcttctttta tggcctgttc atcaggaata gcagcgcaaa taaagacctt actcattttg
      301 ttttaacctc attacagatt tcagggtgaa cgaatccctg ccattgctgg catttttaat
      361 ccgttggtat ggtgttaata tggctggagg attatccagc cggtgtttcg ttattcaggt
      421 acagcgatac tttttttagc gggaggcatt caccagaaat tttttgctcg tctcttgcct
      481 ggaggcagga ttctttactt gcataaattc cggtaatcac attctgtgat tcacccgtta
      541 taagaaaaac cgtcatcatc agtgcaaatg ctgaagtcat tgacgttctc cgaaaatacc
      601 aagttcaaga agagcaattc gggaaagtat ggaattatca ttgagcagat aaggctcata
      661 tttcctcata ttaatggcat cttcagtaaa ctcccggtta ctgagcagaa caccaatatc
      721 aaaacaacct tcagacgtat taacgtttgg taataacgtt tccattatcg cgtcctcaac
      781 aatgaatttt gtgatgcagt gcctggtgcc tccaggtgac gttaaccagt taacaattaa
      841 cgccggataa agagaatcca cccataacac tgtttttggt tttaactgtt ccgcgtgcgc
      901 tcagccgcat tcaccacatc acaaaattca ctttaaaaag ggcggcagag cagtcacgga
      961 gtaaaactga taccgccaaa agtcaccaga aaattgataa cagagggcgt tgcagcggag
     1021 ttgtcactta agcgtatggt caacctgaca acccggtgtc ctcaacgggg gaaggaataa
     1081 ccccgccata cttaccgccg cgccatttcg cggattgcca caaccggaag cgcacgatcg
     1141 aattaaattt aacgacgacc tatacagaga gactaacttc tccgggcgct ttcgtgttat
     1201 gccctgactt ttcagggata tatccttttc agtaaactgt cagtgccgga ttcttatccg
     1261 tgtccggcgc acgcactcta cctcacctgt gaataaatta atgattaatt gatattttgt
     1321 tgtttgattc aactttccca tcggatgtgt gatgctttaa atcacaggaa ttaatactgc
     1381 ttgctgtaaa atgattttca aggggagcta ttcgaatccc tttctttttc attaacaagc
     1441 caaatccttt attaatgatg tccattaatt ccagaaagta tttttcatgt aaatcctggt
     1501 tatcagagag ctgcttctct tcgtacagcc cgataaaggc tcggcgtacg ttaccagata
     1561 tattgtcgat ggtttctttt tctacggtac tcaggtcaag agtcgccagt tgagagcgaa
     1621 ccacattcgc tgccatttcc tggaatggca ttggtaaatc tttaaattcc attattagcc
     1681 tcgttggtta gctattaacg cgggtatgta atcattctgg caatgcttaa tgccgctgct
     1741 ttttccagcc tggtgatatc ctgctccaga gcggacagat tttcagcctg cttagccctg
     1801 gcttcattgg cccatttcag gtcctgcgca gccttaattt tctggtgcat ccactcataa
     1861 agttcatcat cggtatagtc tggcgcgatg atgacgggtt ctcgtttctg catactgatt
     1921 cctcgcggtg ctgtttcgct tatcagccgt tagattttgc cgaactggaa agcgcctgtt
     1981 taaactcact gaagctgagg gcttcttcgc cttcggcaag gccttcgaag tattcttcgt
     2041 aagccttttc catgattgtg ttgaaatcca tatcactcac ctgagtttct ttccagccag
     2101 cgacgggcac cattttcggt tttaaacgtt ttgcttctgg tatacgtcat cgcggtgaac
     2161 gtaccgtcct ggttggggaa cacgccacat accagagatt cgctgttgcc aagatcgata
     2221 gtatccatgc tgacctcatt tccccttaac gccggggtag cggaacaaaa acctgctgca
     2281 tagttattaa agttgaaccc tgccgtcatg ttcttacgcc tcgggctggc tacttaaccc
     2341 ctgaccactg ccgggtaact cgaagtattt ccctgcgttc tgtggggcgg ggtgggttgg
     2401 tatgttgtta aggtaacaag agttaccttt cgagtcaata caatgttgca aaaggtacat
     2461 ttgagggcat aaaaaacccg caatgaatgc gggttctgac tcagtctaag tattgatgta
     2521 tttgtgaaac tttaccttta atggtgtaac caccattcag ttcgatgggt ttgtaaagcg
     2581 gattcagtga caacagatag atgtttggtc cgtcaatcgc aacttttttt agtgttacgt
     2641 ttggcgttcc ttccaattgg attaagatta tttttcccac cagttctcta atgttacttg
     2701 agcatggtgt gatcagcacg gtagatccgt cggggatggt tgggaggccg ttagagtttg
     2761 tcatcgcatc tccctcaaca tgcaataaaa aagagttttc agcggttttt gtcatgacat
     2821 caacccagtt cttaatacca ggaatcttgg ttactggaca actcatatcc caataaccag
     2881 cctgttccca cgttaaaacg ggcaaccggg cgatgttgtc actaatgtaa gggtactgat
     2941 tcagacgcag atcatcggtt ttatcgtgac cgtcctttcc ataaagaatc cattcaggag
     3001 atttggaaag caattttgac agtagataca aattctcacc gtcaggtttt gaagagccat
     3061 tttcccattt tgttacggat acacgagata tgccgattgc tttcgcaacc tgctgttggg
     3121 ttaatccaac gtcttttcga cgattccgaa tacgttcgct gatagtgttt ttcatgtaac
     3181 caatgttact accaagtgat gttgctatgg ttgacattgt tatgtaacta ttgttaccct
     3241 tctgctcgaa ataacaggag agttttatgt tcaaagatga tgttctgcgc tatttcaaaa
     3301 aaaagcgact agtagctgag gctcttggaa tttcacatgt ggctgttgtg cggtggaaag
     3361 cagttattcc caaacttcgc gcaatggaac tggatgaaat tactaacggt gaattgaaat
     3421 acaacccaga actttacaag aagcaggata gcacctcgaa cgaaggaaag aatgattcat
     3481 gaaaatcaag catgaacaca tccgcatggc gatgaatgt
//
LOCUS       DAZGLJ010000319         3496 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000319 DAZGLJ010000000
VERSION     DAZGLJ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.321"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            230..331
                     /locus_tag="ACHK49_004723"
                     /pseudo
     CDS             230..331
                     /locus_tag="ACHK49_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(741..976)
                     /locus_tag="ACHK49_004724"
                     /pseudo
     CDS             complement(741..976)
                     /locus_tag="ACHK49_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1147..1797)
                     /locus_tag="ACHK49_004725"
     CDS             complement(1147..1797)
                     /locus_tag="ACHK49_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO9265209.1"
                     /translation="MPFSFNLSSGNYLSTQDVEVLQRATRDHQMERLTIGERSFSVRY
                     QSAMDAFIVDPVQGELYSGLSHTELADIIRLADSVENQLNGGNSFLDVFSTYMGQVIS
                     EFMHSNDNRIELLQRRLHSCSFLVNIEEMSYIDEALQCPITLAIPQRGVFLRNAEGSR
                     VCSLYDEMALSRIINDGMHHPLSREPITLSMLVAREQCEFDCSIGHFTVRSDCYSV"
     gene            2029..3085
                     /locus_tag="ACHK49_004726"
                     /pseudo
     CDS             2029..3085
                     /locus_tag="ACHK49_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(3304..>3496)
                     /gene="espM1"
                     /locus_tag="ACHK49_004727"
     CDS             complement(3304..>3496)
                     /gene="espM1"
                     /locus_tag="ACHK49_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HFO9265210.1"
                     /translation="NASFGKKMDALCDGMSRAVKNSTTDYIANVLADKFYQKHIAPGV
                     DIVKLRNEIPGYMSRVIQG"
BASE COUNT         1055 a          805 c          657 g          979 t
ORIGIN      
        1 tagattttac aaaactcaat aacatattat atttaaagga aaaatacaga atttacgatg
       61 cttttttata cagatctctc gtgtttttaa ctgattgatt ttgttgaact ctttttttgt
      121 tttgcaggag ttactcctct taccttattg tccactcgat gaccaacaca ttcacaaccc
      181 aaaccagaat atcctatcta tgaagcatgt atccgcacgc agattaatta tggtttacat
      241 tgttctacca caatagtcgc atcctaaatg ggtgcggttg aggatcacca tccttgaaga
      301 ggatgcccgc cactttaact gcatgacagg gctatttaaa caatcactaa aaatcgccca
      361 agagcattct gtttctataa tgtattccaa gagattaaaa aataatatca tacgttattc
      421 ggcagagttc gtatgtacta cggcgtccca accatgtccc aaaacaaaaa tttctgccct
      481 atacacgttc caaaaaattc aacagcaaag agagtctgct gacgagttgt tgattcactg
      541 attaatacgt aaaattgata actgctggaa atcatacaat actcgtactt tcgaaagttc
      601 gctaaccagg tgctacacgc gaggtgttgc agttttcttt acgatttatc attcaatcct
      661 aatccatgca tgacatgtgg tgcctgtggg gtgatcctac ctacgtaatg tggacacagc
      721 cctaatcaag gttctgtttt tcaaaacagt tccgtgctga gacagccaca agtactgtgt
      781 cggcaccacc gattgtaatc acactagatc taattaaaca cagccgtctg cattatttcc
      841 cgactcataa aatattagcc gtaaatgcat tcgactttta gtgagtggaa gaaactttcc
      901 gccctagcat tatcgtaaca gcgccccttt gcactcatcc tgtcatgcag gtatgccgct
      961 tcagaagcgc ctgataatgc gttgaaatca ggacggtggc tacttgctat tcagttcacg
     1021 atacaaaatg ggcaatatcc cttaaggacc agcgaatagc aatgagtcac tttattatca
     1081 agtttgatga ccgctttgat gctaactttt aataagagtc tcttcacaca aaatcgtgta
     1141 ccgagtctac actgaataac aatcactcct caccgtaaag tgaccgatac tgcaatcaaa
     1201 ctcacactgc tctctggcca caagcattga taatgttatt ggctctctgc ttagtgggtg
     1261 atgcatccca tcattaatta tacgagaaag agccatttca tcatataaac tacatactct
     1321 ggaaccttca gcatttctta aaaaaacacc tcgttgagga attgccagcg taatcgggca
     1381 ctgtaatgct tcatctatgt aagacatttc ttcaatatta actaaaaatg aacatgaatg
     1441 taatcgccgc tgtaacaatt caattctgtt atcattacta tgcataaatt cagaaataac
     1501 ctgccccata tatgtactga atacatcaag aaatgaattg cctccattca attgattttc
     1561 aacagaatca gccaatctaa tgatatcggc tagttctgta tggcttaaac ccgaatatag
     1621 ttccccttga acaggatcta caataaaagc gtccatagca ctctgatagc gaacagaaaa
     1681 cgatctttct cctattgtga gtctttccat ttgatgatct ctggtcgcac gttgtaaaac
     1741 ttcaacatct tgtgttgaca agtaatttcc agaagacaaa ttaaatgaaa atggcataat
     1801 tttatcccta aagcatatat cttacttttc gataattaac aaaaagccat cctccacagc
     1861 aatactccat cagaataatt gtaggattat tttaatctaa tttataagca taaaatatat
     1921 actcactaaa tagctgcgca gaatagtaga tcatttagag ggaactcaac ccgattgtgc
     1981 gatctgatca atcgccaaat caatacaaat caccaaccgg actgagcaat gtcgatcata
     2041 ccaccaattt cccgtgacga acgacgccta atacagaaag ccatccataa aaggctgaca
     2101 gccatgctga tgctgcaccg gggcgaccgt gtcagcgacg ttgcccgcac actatgctgc
     2161 gcccgttcct ctgttgggcg ctggattaac tagttcacac agtcgggtgt tgagggactg
     2221 aaatcattac ctgccgagcg agcccgccgc tggccgtttg cgcatatcta cacactatta
     2281 cgtgaacagg taaaatattc actcgcgact ttggctacaa ttattcacgc tggagtacag
     2341 aactgctgac aataaaaatc aatgagataa ccggctgcct gttacatgct ggaaccattc
     2401 gccgctggtt gtcgtctgtg gggcttgtgt ggcgaagggc tgcgccaact ctacgtatcc
     2461 gtgccccgcc taaagataaa aagatggcaa caatccataa cgcactggac gaatgcagca
     2521 cagagcatcc ggtcttttat gaagatgaag tatttatcca tcttaatccc aaaatcggtg
     2581 cggactggaa actgctcggg aagcaaaaac gaggggtcac gccggaacaa aatgaaaaat
     2641 attctctgga cgtagcgctg cacagcggga caggttaagt cagctgtgtg ggcgccaaca
     2701 gcaaaagttc ggcgctgttc atcagactgc tgaagcggct taaagctaca tactgtcgga
     2761 cgaaaaccat cacgctgctc gaggacaact acattatcca caaaggccgg gaaacccagc
     2821 gctggctgaa ggataaaccg aagtgcagag tcatttatca gccggtttaa tctccatggg
     2881 tgaatcattt tgaatagcta tggcagatac ttcacgccac aataacgcgt aatcatcagt
     2941 gccgctcaat gtggcaacta ttgaaaaagt tcgccatttt atggaatccg tcagtccatt
     3001 ccccgaaggc aaacatgggc tggcaaaaat gtgcgatatt aggcgcagct atttagttat
     3061 tccgtcattt ctcccgcccg tgtaaacact actgaatctt gtcgttgtga caaagctata
     3121 aatagtcaat cacagcacgt ttctgcgtac gacgcgctgc aattttcttt acgatttata
     3181 actgtacagg tgaactatga gttatttgga tacatacata tagtgtaacg cctgttgtat
     3241 attttactca tatcttttta caggatgaaa accaatgata ctggcgcata gcctcctcca
     3301 accttacccc tgtataacac gactcatata acctgggatt tcatttcgta gttttacaat
     3361 atcaacaccc ggggcgatat gtttctgata aaacttgtct gcaagcacgt ttgctatgta
     3421 atctgttgta ctgtttttta cagctcggct cataccatcg caaagagcat ccattttttt
     3481 gccaaaagaa gcattt
//
LOCUS       DAZGLJ010000320         3458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000320 DAZGLJ010000000
VERSION     DAZGLJ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.324"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..105)
                     /locus_tag="ACHK49_004728"
     CDS             complement(<1..105)
                     /locus_tag="ACHK49_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265211.1"
                     /translation="MTSREARLVMFLVSLLRRIAFSYYDYKAYNFNIEK"
     gene            393..1709
                     /gene="shiA"
                     /locus_tag="ACHK49_004729"
     CDS             393..1709
                     /gene="shiA"
                     /locus_tag="ACHK49_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO9265212.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDVQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFARLADRFGRRRVYITGALIGMLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAITALLMKDNQRA"
     gene            1811..3265
                     /gene="amn"
                     /locus_tag="ACHK49_004730"
     CDS             1811..3265
                     /gene="amn"
                     /locus_tag="ACHK49_004730"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO9265213.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          821 a          836 c          813 g          988 t
ORIGIN      
        1 tttttcaata ttaaaattat aagctttata atcgtagtaa ctaaatgcaa tacgtctcaa
       61 caatgatact aaaaacataa ctaacctcgc ctccctactg gttataatgc aatgcagtct
      121 atcagaatca tcagggtgca attttgtgca tatgcggact tttatgtttc atatctcgaa
      181 cctgtgggcc ctctgcttaa tccttaaaca acaccagcaa ctcctgcgct tttatcttcc
      241 atcgaatttt tcatgttgcc gctaatcagc cataaaatca tttgcagatg cgctctgtcg
      301 aggtagtctc ataaggttcg tttatagatc gacggcaatg tgagttacct tttccatact
      361 aattataaaa agacagtaca aacaggatca ttatggactc cacgctcatc tccactcgtc
      421 ccgatgaagg gacgctttcg ttaagtcgcg cccgacgagc tgcgttaggc agtttcgctg
      481 gtgccgtcgt cgactggtat gattttttac tctatggcat taccgccgca ctggtgttta
      541 atcgcgagtt tttcccgcaa gtaagcccgg cgatgggaac gctcgccgca tttgccacct
      601 ttggtgtcgg cttccttttc cgaccgctcg gcggtgtcat tttcggtcac tttggtgacc
      661 ggctgggacg taagcgcatg ttaatgctga ccgtctggat gatgggcatc gcgacagcct
      721 tgattggtat tcttccttca ttctcgacca ttgggtggtg ggcacctatt ttgctggtga
      781 cactgcgtgc cattcaggga tttgccgtcg gcggcgaatg gggaggcgca gcgttgcttt
      841 ccgttgaaag tgcgccgaaa aataaaaaag ccttttacag tagcggtgta caagttggct
      901 acggagtagg tttactgctt tcaaccggac tggtttcatt gatcagtatg atgacgactg
      961 acgtacagtt tttaagctgg ggctggcgca ttcctttcct gtttagcatc gtactggttc
     1021 tgggagcatt gtgggtgcgc aatggcatgg aggagtccgc ggaatttgaa caacagcaac
     1081 ataatcaagc ggccgcgaaa aaacgcatcc cggttatcga agctctgtta cgacatcccg
     1141 gtgctttcct gaagattatt gcgctacgac tgtgcgagtt gctgacgatg tacatcgtta
     1201 ccgcctttgc acttaattat tcaacccaga atatggggtt accgcgcgaa cttttcctta
     1261 atattggttt gctggtaggt ggattaagct gcctgacaat tccctgtttt gccaggcttg
     1321 ccgatcgttt tggtcgccgc agggtttata tcacaggtgc gttaatcgga atgttgagcg
     1381 catttccttt ctttatggcg cttgaagcac aatctatttt ctggatagtt ttcttctcca
     1441 taatgctggc aaacattgcg catgacatgg tggtgtgtgt gcaacaaccg atgtttaccg
     1501 aaatgtttgg tgccagttat cgctatagtg gcgctggagt cggttatcag gttgccagtg
     1561 tggttggtgg tggatttaca ccttttattg ccgctgcact catcacttac tttgccggga
     1621 actggcatag cgtcgccatt tatttgctgg ctggatgcct gatttccgca ataaccgctt
     1681 tgttgatgaa agacaatcaa cgcgcttgat agcctggcga agatcatccg atcttcgcct
     1741 tacacttttg tttcacattc ctgtgacata ctatcggatg tgcggtaatt gtatggaaca
     1801 ggagacacac atgaataata agggctccgg tctgacccca gctcaggcac tggataaact
     1861 cgacgcgctg tatgagcaat ctgtagttgc tttacgcaac gccattggca actatattac
     1921 aagtggcgaa ttacctgatg aaaacgcccg caaacaaggt ctttttgtct atccatcact
     1981 gaccgtaacc tgggacggta gcacaaccaa tccccctaaa acgcgcgcat ttggtcgttt
     2041 tactcacgca ggcagctaca ccaccacgat tactcgccct actctctttc gttcgtatct
     2101 caatgaacaa cttacgttgc tgtatcagga ttatggtgcg catatctcgg tgcaaccctc
     2161 gcagcatgaa atcccttatc cttatgtcat tgatggctct gaattgacac ttgatcgctc
     2221 aatgagcgct gggttaactc gctatttccc gacaacagaa ctggcgcaaa ttggcgatga
     2281 aactgcagac ggcatttatc atccaactga attctccccg ctatcgcatt ttgatgcgcg
     2341 ccgcgtcgat ttttccctcg cgcggttgcg ccattatacc ggtacgccag ttgaacattt
     2401 tcagccgttc gtcttgttta ccaactacac acgttatgtg gatgaattcg ttcgttgggg
     2461 atgcagccag atcctcgatc ctgatagtcc ctacattgcc ctttcttgtg ctggcgggaa
     2521 ctggatcacc gccgaaaccg aagcgccaga agaagccatt tccgaccttg catggaaaaa
     2581 acaccagatg cccgcgtggc atttaattac cgccgatggt cagggtatta ctctggtgaa
     2641 tattggcgtg ggaccgtcaa atgctaaaac catctgcgat catctggcag tactacgccc
     2701 ggatgtctgg ttgatgattg gtcactgtgg tggcttacgt gaaagccagg ccattggcga
     2761 ttatgtactt gcacatgctt atttacgcga tgatcacgtt cttgatgcgg ttctgccgcc
     2821 cgatattcct attccaagca ttgctgaagt gcaacgtgcg ctttatgacg ctaccaagtt
     2881 agtcagcggc aggcccggtg aggaagtcaa acagcggctg cgtacaggta ctgtggtaac
     2941 cacagatgac aggaactggg aattacgtta ctcagcttct gcacttcgtt ttaacttaag
     3001 ccgggccgtg gcaattgata tggaaagtgc aaccattgcc gcgcaaggat atcgtttccg
     3061 cgtgccatac gggacactac tgtgtgtttc agataaaccg ttgcatggcg agattaaact
     3121 tcccggccag gctaaccgtt tttatgaagg cgctatttcc gaacacctgc aaattggcat
     3181 tcgggcaatc gatttgctgc gcgcagaagg cgaccgactg cattcacgta aattacgaac
     3241 ctttaatgag ccgccgttcc gataataagt tctgaacctt cttatcaaaa atagatgtga
     3301 aggattaagt aagacatctg gcaaatagcc cgcaataaac gcgggctgtt tgcttccttg
     3361 ggcggatacg agttttatta tcgtcttaat gatttccaca tattaaaagc aagtatgctt
     3421 tcaaaacaca attataaaaa accccaccaa cattataa
//
LOCUS       DAZGLJ010000321         3452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000321 DAZGLJ010000000
VERSION     DAZGLJ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.325"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..538)
                     /locus_tag="ACHK49_004731"
     CDS             complement(<1..538)
                     /locus_tag="ACHK49_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFO9265214.1"
                     /translation="MNNTDLIHLIKRFMRNEHKAVEEVIDSPLSEVANLIKILQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFT"
     gene            complement(507..1715)
                     /locus_tag="ACHK49_004732"
     CDS             complement(507..1715)
                     /locus_tag="ACHK49_004732"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFO9265215.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSALTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNVIATI
                     VAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGCYFSWVDCSALGYPFD
                     KFYRRLINEGRVGIMAGHVYGTEGEGYLRLNVACSREKLYMGLARLASVIKNINQEKE
                     NEQYGSYPSY"
     gene            complement(1715..3295)
                     /locus_tag="ACHK49_004733"
     CDS             complement(1715..3295)
                     /locus_tag="ACHK49_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFO9265216.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMGK
                     FIPVLAIPLIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKEKDDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVAADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLFIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRRAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGLANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPLAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEETAIANIPVHDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEAAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIC"
     gene            complement(3327..>3452)
                     /locus_tag="ACHK49_004734"
     CDS             complement(3327..>3452)
                     /locus_tag="ACHK49_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HFO9265217.1"
                     /translation="KDFQLQRAIHEIERYLYATFKYDSLLEEKLYLSIRIKVLMD"
BASE COUNT         1087 a          787 c          618 g          960 t
ORIGIN      
        1 gagtaaattt tttcaattca gagagtgcaa gcgccaccgc atcaccaaca accagaacaa
       61 cagtagacga gcatgacggt gctaaaccta aattatccgc ctcacttttg acggggattt
      121 caacagataa atcacaactt atcgcaagcg atgagtgatg actgcgggta aaagagatca
      181 aataattacc catttttttc aaactaggca gtgtcgccag gatttccgcc gtctcaccac
      241 tgtttgaaat aagaataaca acatcgtctt tcgccaccat cccaaggtcg ccgtgtaccg
      301 cttccgtacc gtgaacaaaa aacgagggag ttccggtgct ggcaaatgtt gcagcaagtt
      361 ttctggcaat aatgccagat ttcccaacac caataaaaac aacttttccc tggcaagatt
      421 gtaatatttt aattaaattc gcgacttccg aaagggggga atcaataacc tcttctactg
      481 ctttatgttc gttacgcata aaacgtttaa taagatggat aagatccgta ttgttcattt
      541 tctttttcct gattaatatt tttaattaca ctcgcaaggc gcgcaagccc catatataat
      601 ttttctcgac tgcaggccac attaagacgc agatagccct ctccttcagt accataaaca
      661 tgccccgcca taatacctac tctcccttcg ttaattaatc gacggtaaaa tttatcaaag
      721 ggataaccaa gggccgagca atcaacccac gaaaaatagc agccctcagc tggccatacg
      781 atcaattccg gaatattttc atttaaatac tgcaaggtaa attgaatgtt agaggcaata
      841 tgtaccttta acgactttaa ccattcttca cattcattat aggcaacaat ggtcgcaatt
      901 acattcagta ctgatgggga gtttacgaaa tcgcgatacc tcgatatcgt tctgaactta
      961 tgattaaaat tatcgtcagt tgaaatcata tatgatccgg ttagcgcagg aacattaaat
     1021 gttttcgaaa tcgaattaaa caccaccact ttgtcatctt ttattgttag cgctgacgtg
     1081 aaactatgat taaaaacaaa atcactatgt atttcatctg aacataacca aatattatgc
     1141 cttgcacagc atgctgcgat tctgtgaaga tcgtcttccg accagaccgt tcctgtcggg
     1201 ttatgaggat tacataataa caccatcttc gcgcttttaa ttttaagctc aaagtcttgc
     1261 cagttgataa cgtagcctct cccattttct ttcatcaaag aggttgttag catgtttcga
     1321 ccattatgct taatcacatc aaaaaatgcg ttataaacag gcgtgaaaat gagaatatta
     1381 tctccattca atgtcaatat ttctattgcc ttagatattg aatacatcac gcttgggcta
     1441 taggtgatcc attctggttg aatctctgtt ttatattgtt gatgatacca attgactatc
     1501 gaattcagat agtcatcatg ctgccaacga ctgtacccta aaatcgggtg ttccagacgt
     1561 ttttttaatg ttctgatgat agcttccggt atcggaagat ccatatctga aatagagaat
     1621 ggtaatacat cttttacacc aaatctgtct tcacaaaagt cccattgtgt gcaatatgtt
     1681 cctttacgag aaattgaggt attaaaaata tccatcaaca tatccccatg taattatcca
     1741 tatcccgacg tagggcgatg acttttgtac cgataatgac ttgtaaggta ttttgatcaa
     1801 gtttcacaac cgcaattcct ccggcccttt ttattgcagc ctcatctatc aatcccatat
     1861 ccgcgatagt taaacgcaaa cgagttacac aattatccag tgatttaata ttttctttac
     1921 cacccagagc cttaaccatt tgctccgctg cctgcaactc tgatttatca tgaactggga
     1981 tgttagctat tgcagtttct tcgtgagcgt tcagcgtgac aggactggac tcttcacggc
     2041 ctacagtcat caggttaaat ttgagaatgg tccaacggaa cacaaagtaa taaatgaaga
     2101 aataagccac ttcaacacat accgctaatg gccatcccgt tcttgtgcca cgtaagacac
     2161 caaagctaat aaattgaatc agatcgccag taaagcccat cttcacgccc atatatggca
     2221 gaaccatgtt agccaatcca ttcataaagg catggaagac aaacaacagc gggctgacaa
     2281 ataagaacag aaattcaatc ggctcgctga cgccgccaat aattaccgtg agcacaccgg
     2341 aaatcagcaa tgctttgata gcccttctgt tttgagcatg agcagtgcga tacatcgcca
     2401 gcgcggctcc agccagacca tactgaatca tcagtttgcc ctgctccatt ttccccgcca
     2461 aatccagcgg aatgaccgca ttctctttga cgtatgccat aaacatgttc agggtgccgt
     2521 aatactcttc accgccaatc actgccgatc cgccgattgg ggtaaagcgg aaaactgatg
     2581 tcaccaagtg atttaagcca aaaggtatcg tcacgcgttc ggcaacagca tagaagaaat
     2641 aaccaacagg accggaagtt gaaatccagt gaccaatgag catgaaaaga ttgaaaaatg
     2701 gcggccagat aaaaggaatg aacagaccaa atccactcat cacaatcagt gtaatgatag
     2761 gcacaaggcg tgggccacta taaaacccta atgattcagg aatacgtaag ttaacgatct
     2821 ttttatacat gctggcgact aataaccctg ccacaatccc acccaacacg ctggtattgt
     2881 aggactggat cccgagtatg atggtttgcc catgtgtcga catttggtca gcagctacca
     2941 ataagtcgtg ctgtttaaga taaaagttcg ttccaagatg catcgccata aagccaatta
     3001 agccagaaaa agcgccatag gctttatcat ctttctcttt taataatccc aaaggtatag
     3061 ctatcgcaaa caatacaggt aaattaacga aggcaaacaa accaaggcta acaataaaat
     3121 caagtatggt tttaattaac ggaatagcca acactggaat gaattttccc atatcatcgc
     3181 tggctaaacc acttcccagc cctaacatca taccacatac acttaacaga gcgatgggat
     3241 acataaatgc cttccccagg ctctgaaaaa aactccaggc tttcttttgt ttcatactgg
     3301 ttatctcatg aaaatgttat gtataattag tccattaata ctttgatgcg aatagaaaga
     3361 tatagtttct cttccagaag agaatcatat ttaaaagttg cgtacagata tctttcaatt
     3421 tcatgaatgg cacgttgtaa ctgaaaatct tt
//
LOCUS       DAZGLJ010000322         3380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000322 DAZGLJ010000000
VERSION     DAZGLJ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.326"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            88..1239
                     /gene="yiaY"
                     /locus_tag="ACHK49_004735"
     CDS             88..1239
                     /gene="yiaY"
                     /locus_tag="ACHK49_004735"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO9265218.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1404..2942
                     /gene="aldB"
                     /locus_tag="ACHK49_004736"
     CDS             1404..2942
                     /gene="aldB"
                     /locus_tag="ACHK49_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO9265219.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3161..>3380)
                     /locus_tag="ACHK49_004737"
     CDS             complement(3161..>3380)
                     /locus_tag="ACHK49_004737"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265220.1"
                     /translation="SEVCCVAFQQVEVYHIEIMELLTKLMNNSSKTSTVQIKRIKPSI
                     IYRLLLIGLGTPMVIYGLVCPLTIETRE"
BASE COUNT          829 a          837 c          904 g          810 t
ORIGIN      
        1 ttttgataat taataataat gatcatgttt tcgaatgaaa atcctcagta agctgcccac
       61 ccttttttac actttcagga gtgtgttatg gcatcttcaa ctttctttat tccttctgtg
      121 aatgtcatcg gcgctgattc attgactgat gcaatgaata tgatggcaga ttatggattt
      181 acccgtacct taattgtcac tgacagtatg ttaacgaaat taggtatggc gggtgatgtg
      241 caaaaagcac tggaagaacg caatattttt agcgttattt atgatggcac ccaacctaac
      301 cccaccacgg aaaacgtcgc cgcaggtttg aaattactta aagaaaataa ttgcgatagc
      361 gtgatctcct taggcggtgg ttctccacac gactgcgcaa aaggtattgc gctggtggca
      421 gccaatggcg gcgatattcg cgattacgaa ggcgttgacc gctctgcaaa accgcagctg
      481 ccgatgatcg ccatcaatac cacggcgggt acggcctctg aaatgacccg tttctgcatc
      541 atcactgacg aagcgcgtca tatcaaaatg gcgattgttg ataaacatgt cactccgctg
      601 ctttctgtca atgactcctc tctgatgatt ggtatgccga agtcactgac cgccgcaacg
      661 ggtatggatg ccttaacgca cgctatcgaa gcgtatgttt ctattgccgc cacgccgatc
      721 actgacgctt gtgcactgaa agccgtgacc atgattgccg aaaacctgcc gttagccgtt
      781 gaagatggca gtaatgcgaa agcgcgtgaa gcaatggctt atgcccagtt cctcgccggt
      841 atggcgttca ataatgcttc tctgggttat gttcatgcga tggcgcacca gctgggcggt
      901 ttctacaacc tgccacacgg tgtatgtaac gccgttttgc tgccgcatgt tcaggtattc
      961 aacagcaaag tcgccgccgc acgtctgcgt gactgtgccg ctgcaatggg cgtgaacgtg
     1021 acaggtaaaa acgatgcgga aggtgctgaa gcctgcatta acgccatccg tgaactggcg
     1081 aagaaagtgg atatcccggc aggcctacgc gacctgaacg tgaaagaaga agatttcgcg
     1141 gttctggcga ctaatgccct gaaagatgcc tgtggtttta ctaacccgat ccaggcaact
     1201 cacgaagaaa ttgtggcgat ttatcgcgca gcgatgtaat catcatttcc acaacggctg
     1261 gcaaattgtc agccgctttt tcaactatct ctgtaaccct tgcccgtaaa ttcgtgatag
     1321 ctgtcgtaaa gctgttaccg actggcgaag atttcgccag tcacgtctac ccttgttata
     1381 cctcacaccg caaggagacg atcatgacca ataatccccc ttcagcacag attaagcccg
     1441 gcgagtatgg tttccccctc aagttaaaag cccgctatga caactttatt ggcggcgaat
     1501 gggtagcccc tgccgacggc gagtattacc agaatctgac gccggtgacc gggcagctgc
     1561 tgtgcgaagt ggcgtcttcg ggcaaacgag acatcgatct ggcgctggat gctgcgcaca
     1621 aagtgaaaga taaatgggcg cacacctcgg tgcaggatcg cgcggcgatt ctgtttaaga
     1681 ttgccgatcg aatggaacaa aaccttgagc tgttagcgac ggctgaaacc tgggataacg
     1741 gcaaacccat tcgcgaaacc agtgctgccg acgtgccgct ggcgattgac cattttcgct
     1801 atttcgcctc gtgtattcgg gcgcaggaag gtgggatcag tgaagttgat agcgaaaccg
     1861 tggcctatca tttccatgaa ccgttaggcg tggtggggca gattatcccg tggaacttcc
     1921 cgctgctgat ggcgagctgg aaaatggctc ccgcgctggc ggcgggcaac tgtgtggtgc
     1981 tgaaacccgc acgtcttacc ccgctttctg tactgctgct aatggaaatc gtcggtgatt
     2041 tactgccgcc gggcgtggtg aacgtggtca acggcgcagg tggggaaatt ggcgaatatc
     2101 tggcgacctc gaaacgcatc gccaaagtgg cgtttaccgg ctcaacggaa gtgggccaac
     2161 aaattatgca atacgccacg caaaacatta ttccggtgac gctggagctg ggcggcaaat
     2221 cgccaaatat cttctttgct gatgtgatgg atgaagaaga tgcctttttc gataaagcgc
     2281 tggaaggctt tgcactgttt gcctttaacc agggtgaagt ttgcacctgt ccgagtcgtg
     2341 ccttagtgca ggagtcaatc tacgaacgct ttatggaacg cgccatccgc cgtgtcgaaa
     2401 gcattcgcag cggtaaccca ctcgacagcg tgacgcaaat gggcgcgcag gtttctcacg
     2461 ggcaactgga aaccatcctc aactacattg atatcggtaa aaaagagggc gcagacgtgc
     2521 tcaccggcgg gcggcgcaag ctgctggaag gtgaactgaa agacggctac tacctcgaac
     2581 cgacgattct gtttggtcag aacaatatgc gggtgttcca ggaggagatt tttggcccgg
     2641 tgctggcggt gaccaccttc aaaacgatgg aagaagcgct ggagctggcg aacgatacgc
     2701 aatatggcct gggcgcgggc gtctggagcc gcaacggtaa tctggcctat aagatggggc
     2761 gcggcataca ggctgggcgc gtgtggacca actgctatca cgcttacccg gcacatgctg
     2821 cgtttggtgg ctacaaacaa tcaggtatcg gtcgcgaaac ccacaagatg atgctggagc
     2881 attaccagca aaccaagtgc ctgctggtga gctactcgga taaaccgttg gggctgttct
     2941 gatataagaa gctggtcgca ttgggtattc attgcctgat gcgacgctta cgcgtcttat
     3001 catgcctacg ggaacctgac cgtaggccgg ataaggcgta cacgccgcat ccggcataaa
     3061 caaagcgcac gttgttacaa tttacgtttt aaacctacgt gcgcaataac ccagactacg
     3121 ccaacagaag tgcgtttctg gggcgtgttt tgccaggagt ttactctcgc gtttcgatgg
     3181 tgagcgggca aaccaggccg taaatcacca ttggcgttcc gaggccaatc agcaataaac
     3241 ggtagataat tgaaggttta atacgcttta tctgtactgt ggatgtttta ctggagttat
     3301 tcattagctt ggtcaatagt tccattattt caatatgata aacctcgacc tgttgaaaag
     3361 ccacgcagca cacttcagaa
//
LOCUS       DAZGLJ010000323         3363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000323 DAZGLJ010000000
VERSION     DAZGLJ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.327"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(116..2377)
                     /gene="katE"
                     /locus_tag="ACHK49_004738"
     CDS             complement(116..2377)
                     /gene="katE"
                     /locus_tag="ACHK49_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO9265221.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            2560..2823
                     /gene="cedA"
                     /locus_tag="ACHK49_004739"
     CDS             2560..2823
                     /gene="cedA"
                     /locus_tag="ACHK49_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO9265222.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            3112..>3363
                     /locus_tag="ACHK49_004740"
     CDS             3112..>3363
                     /locus_tag="ACHK49_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310463.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265223.1"
                     /translation="MFSTNLIQSNYGDLNIKTLAFDSFKERLESTMAALTFFISTGQC
                     DCDEIAESNFNRMIAYMSNINYDASKPGAPALSFDTYLQD"
BASE COUNT          834 a          785 c          838 g          906 t
ORIGIN      
        1 caatacattg aatttatacg actttgtagg ccggataagg cgttcacgcc gcatccggca
       61 taaacaaagc gcactatgta aatcatttga ggcggcgcaa ttgcgccgcc tcccatcagg
      121 caggaatttt gtcaatctta ggaatgcgtg accacacgcg gtgtgctgcc atcagcgtta
      181 gcagttcatc cataaaacta ccgtcagcgc tgtcagcttc cacaatccct tcttcaccct
      241 ggtcagcgac cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt
      301 taaggtgttt gtaggcttcc atcaggtagt agttggcatc gccgttgtca gcgatatccg
      361 cgatattgcc gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg
      421 tagcggctat aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga
      481 gcagtttggc atgaacgcct ttggccttca gggccttgag aatggccaga aggtctgccg
      541 atctcacttc atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa
      601 tggcgtacaa acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga
      661 tattcagctg gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag
      721 tgagatcaat atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt
      781 tgcttaactc aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta
      841 gccagaacag acgcggatgg gaataatatt cgccaaacga tggactgcgc tcacgaactt
      901 tatttccttc cacgcgttcc tgatatgatt caaaaccgcc gcgtttcggt cccggcggtg
      961 tttcgcgcgg ccagttatcg ttgatcgagt tcggttcgta attcgccggg ttagtgtcga
     1021 tccccatacg atgcatgcca tcacgctgga agttgtggta agggcaggtc ggacggttaa
     1081 tcggaatctc atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga
     1141 acaaacgtcc ctgcaacagc ggatcgttgg tgaagtccag accaggaaca atatgacctg
     1201 gatggaatgc tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca
     1261 ttttgccgac acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa
     1321 gatcgaagtc gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt
     1381 ccggaaaatc gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac
     1441 gtccggtgag tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc
     1501 agtggaaacg tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac
     1561 cgaagccttc catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca
     1621 cgttgtgcag agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt
     1681 gcccttgtgg aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt
     1741 tatgcgcatc ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct
     1801 cttcagtata gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag
     1861 cgccaccctg aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga
     1921 ggaaatccgc tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg
     1981 ctgatccgcg tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt
     2041 tctcgcgcag aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt
     2101 tttgatcatc ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt
     2161 tgcgtacgtc ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc
     2221 caggggcggt aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt
     2281 cctcaggtgc cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact
     2341 ggtgctgatg tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt
     2401 actgaaaggg ccgctgtttt ggtcaaaaaa ccctctaact atagaccatc agccagatcc
     2461 cgcttcggag actattctgg aaacggtgtt atgaaataca cgcaaagtgg gacgtacagg
     2521 ggctaaatcg gctaaactac agtttttctg atttttaata tgcgtttagt gaagccagta
     2581 atgaagaaac cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa
     2641 cccgcgccgc cagaacatgc gataaagatg gattcttttc gtgatgtctg gatgctgcgt
     2701 ggcaaatatg ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact
     2761 gtgcctgaat ccgcccagcg ttgggcaaat cagatccgcc aggaagggga agtgactgag
     2821 taaagattac gtacctcccg gttttgccgg gagttacatg acctgaaatt ctgtatttca
     2881 ttgctacatt cattcaaacc accagtaaca aaaagctgcc tttgacgatc tattgctata
     2941 aataagtgat cttttttctt taattgttta tttattttaa aatggttaag ttttgctaat
     3001 catgatgagc tatttttgat agttcatcct gtggcgtcgc gcttaaactt atattgtaaa
     3061 tgacttagat ttaaatgaga taataatttt gttaacaata tgaggtggga gatgttttct
     3121 acaaacttaa tacagagtaa ttatggtgat ttaaatatta aaactctggc ttttgactct
     3181 tttaaagaaa ggcttgagtc taccatggcg gcactcactt tctttatatc aacgggtcaa
     3241 tgtgactgcg atgaaatagc agaatcaaac ttcaatcgta tgattgccta tatgtctaac
     3301 attaactatg atgcctcaaa gccaggagcg ccagcactta gttttgatac atatctgcag
     3361 gac
//
LOCUS       DAZGLJ010000324         3323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000324 DAZGLJ010000000
VERSION     DAZGLJ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.328"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..276
                     /locus_tag="ACHK49_004741"
     CDS             <1..276
                     /locus_tag="ACHK49_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO9265224.1"
                     /translation="VWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGAVVSFKGYYGD
                     LAIVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK"
     gene            328..711
                     /gene="gpFI"
                     /locus_tag="ACHK49_004742"
     CDS             328..711
                     /gene="gpFI"
                     /locus_tag="ACHK49_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO9265225.1"
                     /translation="MATKEENQKHLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMPGDESGPEQNHTDDGEQLHNTDATDDVKAVRVRKCLHVMGYCPETGRPVELT
                     YRGMRVLVPSPLATAMIQHGTAEHA"
     gene            704..1057
                     /locus_tag="ACHK49_004743"
     CDS             704..1057
                     /locus_tag="ACHK49_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO9265226.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGSGVRIEGSCPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            1072..1605
                     /locus_tag="ACHK49_004744"
     CDS             1072..1605
                     /locus_tag="ACHK49_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9265227.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMPGRRPDSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            1602..1997
                     /locus_tag="ACHK49_004745"
     CDS             1602..1997
                     /locus_tag="ACHK49_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO9265228.1"
                     /translation="MKHTDIRAAVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDSWMESRIYPAMSAIPALAGMITTM
                     VQQGYDYRRDDDMALWSSADLTYSITYEM"
     gene            2005..2757
                     /locus_tag="ACHK49_004746"
     CDS             2005..2757
                     /locus_tag="ACHK49_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9265229.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYTGKGDAYANPLSDDNWQRLAQVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITP
                     VTAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSTGTIAVKDMAITVTG
                     VKAGKVSIPVISGNGQFATVAEVTVTEAGAAG"
     gene            2771..3202
                     /locus_tag="ACHK49_004747"
     CDS             2771..3202
                     /locus_tag="ACHK49_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO9265230.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTESPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGAVHVDTDITEVAKNNALTDDDFSAGKSSTAS"
     gene            3229..>3323
                     /locus_tag="ACHK49_004748"
     CDS             3229..>3323
                     /locus_tag="ACHK49_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO9265231.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQ"
BASE COUNT          769 a          773 c         1055 g          726 t
ORIGIN      
        1 gtctggcgtc tgctgaatgg ttttaagctg ttccgcgaaa aactggatac ccgtcgcggc
       61 tcaaattcac aactcgaaac ggcagtgaaa gacctggggg cggtggtgtc tttcaaaggg
      121 tattacggcg atctggccat tgtggtggcc tcttcccgtt atccgaaaca ctggctgacg
      181 gtaggggatc ccgcccgtga atttaccatg acgcagtccg cgccgctgat ggtgttgccg
      241 gacccggatg agtttgtggt ggtgcaggtg aaataatccg tgagcggggg cgaaatgccc
      301 ccgtgtcttt tttcacaggg ggctgatatg gcaacaaaag aagaaaatca gaaacatctt
      361 cgtcaactgg ctggcctgct ggggcgcgag gcggatatgt cggggagtgc tgcggatatt
      421 gcgcaacgtg tgtctgagtg ggaagaggag cttgctgctt cccgggaggg cattatgcct
      481 ggtgatgaga gcgggcctga gcaaaatcac acagacgatg gtgagcagtt gcacaacact
      541 gatgctacgg atgatgttaa agcggtccgt gtgcggaaat gcctgcatgt gatggggtat
      601 tgcccggaga caggccgtcc cgttgaactg acgtaccggg gcatgcgtgt tctggtgcca
      661 tcaccactgg cgacagccat gatacagcac ggaacggctg agcatgcgtg attttcagaa
      721 tgcctttgat gctgcccttg ccggggtgga cagcacgatt gttgaagtga tggggctctg
      781 tgcgcagttc acctcggggg cacagtgtgg cagcgaagtt cagggggttt ttgacgatcc
      841 ggagtcgctg gggtttgccg gtagcggggt ccgtattgaa ggaagctgcc cgtcattatt
      901 tgtacggacg gatacggttc gtgctgtgcg gcgtggtgac acgctgacca ttaatggtga
      961 gacattctgg gtggatcgtg tttctccgga tgacgggggc agttgttatc tctggctcaa
     1021 ccgtgggcaa ccaccggcag ttaaccggcg acgataaacg cagggtgaat tatggcgata
     1081 aaagggcttg atcaggcgat tgaaaatctg agccgggttc gtaaaaacgc cattccggcg
     1141 gcttcagcaa tggccattaa ccgcgtggcc acaacggcga ttaatcagtc ttcgtcacag
     1201 gttgcccggg agaccagggt gagccggaaa ctggtaaagg aacggtccag actgaaacgg
     1261 gccacggtca gaaatccgaa tgccagaatt atcgttaacc gcggtgatct cccggtgatt
     1321 aagctgggga tcaggatgcc ggggcgtcgt ccggacagca tactcaaagc cggtcagcat
     1381 cggtatcagc gggcatttat tcagcgatta aaaaatggtc gctggcatgt catgcagcgt
     1441 gtggccggga aaaaccgtta ccccattgat gtggtgaaaa tcccgatggc ggccccactg
     1501 aaacaggcgt ttgatgagaa tgttgaccgt atccggcgtg aacgcctgcc cggagaactg
     1561 gcatatgcgc tgaaacaaca actgaggatt gcgataaaac gatgaaacat actgatatcc
     1621 gtgctgcagt gctggatgca ctggagctgc atgaacacgg ggcgacgctg tttgatggtc
     1681 gccccgttgt ttttgacgaa gaggattttc ccgcggtcgc ggtttatctg acggatgcag
     1741 agtataccgg tgaagagctg gatgcggata cctggcgggc cacactgcat attgaagttt
     1801 tcctgcctgc tcaggtgccg gattcagagc tggattcgtg gatggagtcc cggatttatc
     1861 cggcgatgtc cgcgatccct gcactggcag ggatgattac cacgatggtt cagcagggct
     1921 atgactatcg tcgtgatgac gatatggcgt tatggagctc tgcagatttg acttattcca
     1981 ttacatacga gatgtgagga cgatatgcca acaccaaatc ccctggcgcc ggtaaaaggt
     2041 gccggtacca ctctgtgggt ttacaccggc aagggtgatg cttatgcaaa cccgttgtca
     2101 gacgataact ggcagcgact ggcacaggta aaggatctga cgccgggcga gatgacggca
     2161 gaaccctacg atgataacta cctggatgat gaagacgcgg actggactgc gaccgggcag
     2221 gggcagaagt ctgcaggaga taccagtttt acgctggcct ggaaaccggg agaagaaggt
     2281 cagaaagggc ttataggctg gtttgaaagc ggggatgtgc gggcctataa aatccgtttc
     2341 ccgaacggca cggtggatgt gttccgtggc tgggtcagca gtatcggtaa ggccgtaacg
     2401 gcgaaggaag tgatcacccg tacggtgaaa gtgaccaacg tgggtaaacc ttctgtggcg
     2461 gaagaacgca gcgaaattac gccggtcact gcgattaagg tgacgccgac atctggtacg
     2521 gtggcaaaag ggaaaacaac aaccctgacg gtttcttttg agccggaaag tgcaaccgac
     2581 aaaacgttca gagcggtttc cgccgatccg tcaacgggaa ccattgctgt gaaagatatg
     2641 gcgatcactg tgacgggggt taaggctgga aaagtgagta tccccgtgat ttccggtaat
     2701 ggtcagtttg ccacggtagc tgaagtcacc gttactgaag cgggcgctgc agggtaaacg
     2761 gaggtaatac atgtttctga aaaccgaaca atttgaatat aacggtgtgt ccgtcacgct
     2821 ttccgagctg tctgcgctgc agcgtatcga gcatcttgcc ctgctgaaac gacgggcaga
     2881 acaggctgaa gccagcggta acctgcaggt gagcgtggaa gaccttgtca gaaccggcgc
     2941 gtttctggtg gcgatgtccc tgtggcataa ccatccacag aaaacggagt caccatcaat
     3001 gaatgaggct gtgatgcaga tcgaacagga ggtgctcacc acctggcctg ctgatgccat
     3061 tgcccgggcg gaagacgtgg tgttgcgtct gtccgggatg agcggggctg ttcatgtgga
     3121 tacggatatc accgaagtgg cgaaaaataa cgcgcttact gatgatgatt tttctgcggg
     3181 aaagtcttcg acggcgagct gaactttgcc ctcagactgg cgcgtgagat ggggagaccc
     3241 gactggcgcg ccatgcttgc cgggatgaca tccaccgaat atgccgactg gcaccgtttt
     3301 taccgcacgc attattttca gga
//
LOCUS       DAZGLJ010000325         3314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000325 DAZGLJ010000000
VERSION     DAZGLJ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.329"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            103..360
                     /locus_tag="ACHK49_004749"
     CDS             103..360
                     /locus_tag="ACHK49_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265232.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            430..708
                     /locus_tag="ACHK49_004750"
     CDS             430..708
                     /locus_tag="ACHK49_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265233.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            710..1765
                     /locus_tag="ACHK49_004751"
     CDS             710..1765
                     /locus_tag="ACHK49_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9265234.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            1766..2131
                     /locus_tag="ACHK49_004752"
     CDS             1766..2131
                     /locus_tag="ACHK49_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9265235.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRHGNTYFISEAGKRYRRDVALIVR
                     QQRLKLNLSGRLVIKIIAEPPDKRRRDLDNILKAPLDVLTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELECA"
     gene            2128..2817
                     /locus_tag="ACHK49_004753"
     CDS             2128..2817
                     /locus_tag="ACHK49_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO9265236.1"
                     /translation="MNNHYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKA
                     AFSKQSVAKVD"
     gene            3013..3088
                     /locus_tag="ACHK49_004754"
     tRNA            3013..3088
                     /locus_tag="ACHK49_004754"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3046..3048,aa:Ile,seq:cat)
     gene            3179..3252
                     /locus_tag="ACHK49_004755"
     tRNA            3179..3252
                     /locus_tag="ACHK49_004755"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3213..3215,aa:Arg,seq:tct)
BASE COUNT          846 a          764 c          905 g          799 t
ORIGIN      
        1 ggacggcgcc ggaatagaat caaaaatact taagtagcgc gcagggagaa gagggatgga
       61 ccccgaacag gggagtgcta tttatctgga aggattctgt tgatgagaat cgaagaatta
      121 cgtgaaattt ttagtgaaga tggcctctat actgtgcgcg ttgagaaggg cgctattgtc
      181 agccactgcc gtattaaatg tttacagtct caacaaagga agagtggagc tgcgttaatt
      241 cattttgtgg atgggcttgt gacggatggt tttattttgc gtgcaaatga atttgtcaca
      301 tcgttgccgt ctctgaaaga agctgggatt aaggctggtt tttctgcttt tgaagattag
      361 tgaattcatc tacaattcag cgcagggctg aacccctgtt gagtaacact gtgccaccgg
      421 agaaagccga tggcgcaaaa ttccagacta cacaattctg ataattcagc cgtctttgcc
      481 agcaggcacg ggcggcgttc tcatgcattc aaatctgact ggttccggca cgccccatgc
      541 actgaagaac aggccgaatg gctgattcag aactaccgca gacgtgggta tgagtttagg
      601 aaagccctca gcctcgatta tcgtcactgg ataatctacg tcaggctgcc gtactccgag
      661 cgcccaccgc gtccgtcccg cacattccag caacgcatct ggaggtaacg tgcgggtatt
      721 acttcgacct gttccggtac cggaacttgg gttggtggtc cttaaaccag gccgtgaatc
      781 catgcaggta ttccataacc ctcgagtgct tgtggagccg gaaccgaaaa gcatgcgcgg
      841 tctgccgtcc ggcatcgtcc ctgccgttcg ccagccgctg gcggaggata aatcattact
      901 gccgtttttc agcaatgagc gtgtgattcg tgctgttggt ggcgctggtg cactgtctga
      961 ctggctgttg cgtcatgtca aatcctgcca gtggcctcat ggtgactatc atcacagtga
     1021 aaccgttatt caccgttatg gtaccggcgc aatggtgttg tgctggcact gcgacaacca
     1081 gctgcgtgac cagacatccg aatcactcga gcaacttgct catcaaaacc tgtcagcatg
     1141 gatgattgac gtcatcggtc acgcaataag cggtacgcag gagcgtgaat tatctctggc
     1201 tgaattatcc tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg aagcggtatt
     1261 acgtcgttcg ctggggttgc gtgcggaaaa aatccgctca atgtaccgtg aaagcgacat
     1321 cgtaccggga gagcagaccg ccaccatcat actgaagcag cgcacaaaaa atcttgcgcc
     1381 gctgcctcac gcccaccagc aaaacccgcc acaggaaaag acggtggtca gcattgccgt
     1441 tgatccggag tcaccggctc agtatctcca gcgccagaaa ccacaacggg aagagatgcc
     1501 tgtatacacg cgctgggtaa aaacgcagaa atgcatgacg tgcggtaatc aggcagatga
     1561 tccgcatcac atcattggtc atggactggg agggatggga acaaaggctg atgatttgtt
     1621 tgttattccg ctgtgccgta aatgccatag cgaactacac gccggggtaa aagattttga
     1681 agaaaaacac ggcagccagc tgttgttgct gattcgtttt ttaatgcacg cgagaaattc
     1741 gggtgtcctg aagtggaaag catgaatgac tgaacggata gaatttgttt tgccttaccc
     1801 gccgacggtg aatacctact ggcgacgtca tggcaatacg tatttcatct cggaagccgg
     1861 aaagcgttat cgccgtgatg tggcgctaat tgttcgccag cagcggctga aattaaacct
     1921 gtccggaagg ctggtgataa agattattgc agagccaccg gataagcgcc gtcgtgacct
     1981 ggacaatatc ctgaaagcac cactggatgt gctgacgcat gccggacttc tcatagacga
     2041 cgagcagttt gatgaaatca atattgtgcg cggtcagctc gttcctggtg ggcggctggg
     2101 gataaaaatc acagaactgg agtgcgcatg aataaccact atttacagtt tgtgcgtgag
     2161 cagctcatta tcgccaccgc tgatttgagt ggggcaacaa aaggtcagct tgaagcctgg
     2221 caggagaatg ccatgttcga tacagggcgt tacaggcgaa aaaaaatccg gtaccgcgat
     2281 gaagtgactg gaaaaatgat aacgcgggat aatccaccaa tcccgggaaa gcaatcgctg
     2341 gcgaaggtga cgtcaattcc tctggtcagt ccggttgagt tttcgacatc atcgtggcgg
     2401 cgggctgttc tgtctcttga agaacatcat aaagcctggt tgttgtggtg ttacagcggg
     2461 agtatttgtt gggaatatca gatcgcgata acacagtggg cgtggaatga atttaatact
     2521 caatccggta ccagaaaaat tgctgggaaa acgcaggaac gcctgaaaaa attaatctgg
     2581 ctggcggcgc aggcagtaaa agcagaactt tttggtgggg aaggttatga atatcaggag
     2641 ctggcattac tggcgggagt gacaactaaa aactggtcca aaacatttac tcgtcactgg
     2701 gttgcaatga aacacatttt tcaccgactg gatagtgagg ctttattatt tgtaatgaga
     2761 acacgttcaa aacaaaaggc ggcattttca aagcaaagtg ttgcaaaagt agattgaaag
     2821 gcatatattt catgcaaatc tgatattttg ccgattttgt acgtgatggc aaaagcaaac
     2881 aaaacccgcc cacaagcggg ttttgttgtg ccacttatct cggatagaca tggtgaatgc
     2941 gctggtggag gagataaggg tgatttttgt atgcttgcaa cattgatttc gtaacgttat
     3001 tatcctgcgc ccggcccttt agctcagtgg tgagagcgag cgactcataa tcgccaggtc
     3061 gctggttcaa atccagcaag ggccaccaac cgccactagc catcaggaaa gagcgtcaac
     3121 cctttaagtt gagtgtgcga ggttcgagtc cccggtggtg gtccattatc ggtattctgc
     3181 gttgttagct cagcaggaca gagcaattgc cttctaagca atcggtcact ggttcgactc
     3241 cagtacaacg cgctacactt attttcccgg ctcgcttttg cgggcctttt ttgtatctgc
     3301 gccacgcccg gcgc
//
LOCUS       DAZGLJ010000326         3307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000326 DAZGLJ010000000
VERSION     DAZGLJ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.330"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..621
                     /locus_tag="ACHK49_004756"
                     /pseudo
     CDS             1..621
                     /locus_tag="ACHK49_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            976..1542
                     /gene="slp"
                     /locus_tag="ACHK49_004757"
     CDS             976..1542
                     /gene="slp"
                     /locus_tag="ACHK49_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO9265237.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            1698..2228
                     /locus_tag="ACHK49_004758"
     CDS             1698..2228
                     /locus_tag="ACHK49_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO9265238.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(2270..2917)
                     /locus_tag="ACHK49_004759"
     CDS             complement(2270..2917)
                     /locus_tag="ACHK49_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO9265239.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGIAGVKGVSIS"
     gene            complement(2981..>3307)
                     /gene="hdeB"
                     /locus_tag="ACHK49_004760"
     CDS             complement(2981..>3307)
                     /gene="hdeB"
                     /locus_tag="ACHK49_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO9265240.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
BASE COUNT         1054 a          689 c          612 g          952 t
ORIGIN      
        1 gatactaatt ttattgctga acaacgagct gacttaccaa ctaatataaa agatattcaa
       61 agcctgtatc aaaaaatgac gaagttatat attgagcaca gtgaaaataa aaacaggatg
      121 aaagtctttg ccggaaccaa ctttattgat ttcaatatga caggacagaa cctgtccggg
      181 ttcgttctaa ccttgtcaag gttttatttc gaagatctac ttaatataaa tttcactgac
      241 gaaaatttag gtgacactat tttttacata aagaacaccc tacccccaaa ttatataaag
      301 atggacaata tcttgacaaa caaatcgaag gtttattttc aacattatta accattaatg
      361 acaacttttt acgagcaaaa gcggaaattg cttcgacaat aattaaattt ttagaggcca
      421 ggataaccaa tctttcatac aatgacatat taacatatca gcaagaattc caaaaacaat
      481 gttataagca agttaaagcc tttacgacac tctctcgata caataaaatt caaacatggg
      541 cagaaatgtc tgaatatcag ttcgaagtat ttcaatacga gacgcttaac cccaaaaaaa
      601 tgtcacacac gccttattta aagaggccac ttcctaatga aaaagatata aattatgggg
      661 tggaaataga aataccatct ggcaaaagaa ttcgcttatc aaatcattat cagaatataa
      721 taccataata ttgattatta gcactttata attattgaat aaaaatagat tttatgtaat
      781 tttaaaacaa aggattatat tatggggcga tggatactca tgccacgccc caaaatcaat
      841 ggaattgaac taaacaaaag ggatataaac atcagacagg tttacgttac tatcaggcat
      901 atcacctcag aatcagatga aaactataaa gaaatatcta ttatggcttt aatatttgtt
      961 gataaggata gtaacatgaa catgacaaaa ggtgcactca tcctcagcct ttcatttttg
     1021 cttgccgcat gtagttcaat tccgcaaaat atcaaaggca ataaccaacc tgatattcaa
     1081 aaaagttttg ttgctgttca taaccagccg gggttatatg ttggtcaaca agcgcgcttt
     1141 ggtgggaagg ttatcaacgt tatcaatggc aaaacggata cgttgttaga aatcgctgta
     1201 ttaccgttgg atagctatgc gaagcctgat attgaagcca actatcaggg ccgactgctc
     1261 gccagacaaa gcggcttcct tgatccagtg aactatcgta atcactttgt taccatcctc
     1321 ggtaccattc agggtgaaca accaggcttt atcaataaag tcccgtataa cttcctggaa
     1381 gtgaatatgc agggcatcca ggtgtggcat ttgagagaag tggttaatac cacctataac
     1441 ctgtgggatt acggctatgg tgcattctgg ccggaaccgg gctggggtgc gccttactac
     1501 accaatgcgg tgagtcaggt aacacctgag ctggtcaaat aacaccaccg aaagatgcag
     1561 atgctttctc agcatctgca tcatgtatta cataaaatta atatacagta agctaactat
     1621 tattattata agccctgtcc tgttaattac ctttggcaaa ctgattataa agttaatgtc
     1681 cgcaccagga gtcggttatg tttcttataa ttaccaggga tacgatgttc ttcaccgcga
     1741 tgaaaaacat tctgagtaaa ggtaatgtcg ttcatataca gaacgaagaa gagatcgacg
     1801 taatgttgca tcagaatgcc ttcgtcatta ttgatacatt aatgaataat gtatttcatt
     1861 ctaattttct cactcaaatt gaacgattaa aacctgtcca tgtcattgtt ttctccccct
     1921 ttaatattaa acgctgtctg gggaaagtac cggtgacctt tgttccgcgg actatcacta
     1981 tcattgattt tgtcgcactc atcaatggca gttactgctc tgtgcctgaa gcgaatgtgt
     2041 cactttcgcg caagcaacat caggttctga gctgcattgc gaatcaaatg acaactgaag
     2101 atattctgga gaaactgaaa atatcgctaa aaacgttcta ctgccataaa cacaatatca
     2161 tgatgatcct caatcttaag cggatcaatg agctggtacg ccatcagcat attgattatc
     2221 tggtgtgaat ttcaggctta cggtgagtct ggctacgctg ccacacagat tagctaattg
     2281 aaacgccttt cacccctgct atacctttta ataatcgcaa caggtcttct atcgacgttg
     2341 tggcatgaag ctggatatca atggcgacca cttcatgatc ttcattttcc tgcaacgaaa
     2401 ctaaatccgt ttttattttt tgctgcttaa accagtcgag cattgatacc gtattaccgt
     2461 tcactaatgt cagctgtaaa tggtaatttt tgttcatcag gcggaaggtt aattgatgga
     2521 agacttccag caccaacaag gtcatcactg aaccataaat acccagttcg tacataccgc
     2581 tgccaataac catacctatg gcggcggtca cccagatatc cgctgccgtc gtcagaccta
     2641 caatattttg gttacgaacg agaatgttac ctgcaccgat aaacccgacg cccgtcacca
     2701 cctgagcagc aatacggctg gggtcgagtc cgacgtgatc taaagacagc acgtcagcaa
     2761 aaccatattt cgaaacaatc ataaacaggg cgcttcccat gccaattaat acatgtgtgc
     2821 gtaaccctgc tcctttgccg cgcatttgcc tttccatgcc aatagcgcca caggcaattg
     2881 ccgcgagtat aagacgtata ataaattctg ctgtcatata aaaatgcaaa aaggagcagc
     2941 aagatggctc aacttgctac tcctttttac ttgtacctca ttaattcggc aagtcattag
     3001 atgcttgatt tttgaaggta tacaaatttt tctgcgggtt tttcttacag tattcgatca
     3061 ctttaggaat ttgagtgaga tcggtttcat ttaaagtaac ggtatcgcca cctttatata
     3121 ctgtttcttc atgcagcatc caccatgcaa ccggggtcat tgcttttgga ttcagatcaa
     3181 taaattcctg gcaggtcata tctttagcgg attcattggc tgccagcgca gattgtgcgt
     3241 tcaccagtga caaagccgct acagcgccca taaaaataaa cgctttacgg agagatgaaa
     3301 tattcat
//
LOCUS       DAZGLJ010000327         3285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000327 DAZGLJ010000000
VERSION     DAZGLJ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.331"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..320)
                     /locus_tag="ACHK49_004761"
     CDS             complement(<1..320)
                     /locus_tag="ACHK49_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO9265241.1"
                     /translation="MRKLYAAILSAAICLAVSGASAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDIRWHEDSVRNRWFSVMAG
                     PSVRV"
     gene            complement(391..>3285)
                     /locus_tag="ACHK49_004762"
     CDS             complement(391..>3285)
                     /locus_tag="ACHK49_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO9265242.1"
                     /translation="PRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTAL
                     VGVQVDLEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWC
                     LWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQR
                     KAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSA
                     LKDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGL
                     WLIKTELLETQTVDFSVGAEGLRHVPGDVIEIFDDDYAGISTGGRVLAVNSQTRTLTL
                     DREITLPSSGTTLISLVDGSGNPVSVEVQSVTDGLKVKVNRVPDGVAEYSVWGLKLPT
                     LRQRLFRCVSIRDNDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQH
                     LTAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFT
                     QLALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYD
                     PTVQFEFWFSETRITDIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGK
                     SAFVEGVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDA
                     NDKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNET
                     PMFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGRVNANAG
                     TLNNVTVNENCTIKGMLEATQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTVTISDD
                     HNFDRQIIIPPIIFNGIAYSDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPG
                     SYSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
BASE COUNT          687 a          993 c          811 g          794 t
ORIGIN      
        1 acgcgcacag acggccccgc catcacgctg aaccagcggt tacgcacgga atcttcatgc
       61 cagcggatat cgctgtaacg ggtaagctgg cgattcttgt ctcctgcata gctgaatgac
      121 gtcaccagcc ccagtgtgtc cgtaaactca taacggtatt tcacgttaat cccgttcaga
      181 tcatcgctgc cgggaacgtt cgtcgaggca tgaagatacc ccgcgctcag cgtggactga
      241 tgttcagatg cccatgcaga cgcaccggat acggccagac aaatagctgc ggacaaaatg
      301 gctgcataaa gtttacgcat aattacctct cgcttttctg caataaaaaa ggcgtcattc
      361 ctgacgccct ttattggggt tataaatatt tcaacgaata ctgataccgg aagccgcttt
      421 tttggtcaca atcaccgtac agtcggtgat attccctgcc ccctgattgc ctttctggaa
      481 aatcttaaac tccagagtga cgctaccacc accactaggc atatcaataa ctgcactgta
      541 actaccggga atggcccctt tagtttctct ggatgcgatt aatacaccgt ttttgcgaac
      601 ttcaaaacca taacccgtgt atcttgtacc tcccgggtta ttaccacttc ccggatcgct
      661 atacgctatt ccgttaaaga taatgggcgg aataatgatt tgacggtcaa agttatgatc
      721 atcgctgatg gtgactgtaa ccgtcccgtt tggtgtttcc gtattacccc acgtaccagc
      781 ctgtttcggg aatgatttgg atacagcttt aacgaagtca cctctgacct gagtcgcctc
      841 cagcatgccc ttaatcgtac agttttcatt taccgtgaca ttgttgagcg tcccggcgtt
      901 cgcattcact ctgccactga tatccgcatt tttagcggtc agctttccgt ctgatgtcag
      961 ggaaaatacc ggaggactgc caccgctggt aatggtgggg gctgtcaggc gcttcaggaa
     1021 cacgtcgttc atgaatatct ggttgccctg cgccacaaac atcggcgttt cattcccgtt
     1081 tgccgggtca ataaacgcga tacggttagc ggcaaccaga aactggctca gcttgccttc
     1141 ctccgtgtcc tccatgctga ggccaatacc cgcgacataa tgtttgccgt ctttggtctg
     1201 ctcaattttg acgccccaca tggcattcca tttatcgtta gcgtccttcc actctttcga
     1261 aaactcatcc agtctgctgg cgttatcctc cgtcaaatca actttttcca gcagctcttt
     1321 accgagatgg gattcggtta tcttgccttt gaaaaaatcc aggtaacctt ccgcatcatc
     1381 gctcgcccga ccgacgccct ccacgaatgc cgatttgcca acggtgttca cactgcggat
     1441 ataaaagtaa taatcatggc ccggtttgat attgatactg gcggctatcc agtacagcgc
     1501 cgtaccaaga tagcgggctg tggtttcaac ctgcctgata tcggtaatcc gcgtttccga
     1561 gaaccagaac tcaaactgta ccgtcgggtc ataaacggca agatgcggcg tggcggttat
     1621 ctgaaaatag cccggcgtca gctcaatcct cgacggtgct gccggtgcgg caatccggaa
     1681 cgataccgac gccggatcgc cctgctgccc ccacgcattt accgcccgga ctgtcagcct
     1741 gtagttcccc agcgccagtt gcgtgaagcg gtatgtggtt tccgtcgtcc gggccgtgct
     1801 gaccagccgc tcactgccgt catccgctgt tacggtcaga cggagcagga agctcacgcc
     1861 cttcaccacc ttcggcgtgt cccagcgcgc cagcacctga tattccccgc tgtctgcggt
     1921 gacttcggcg gtcaggtgct gcaccgctgg cggcgtgaca ccattcaccg tgccgctctg
     1981 gtcgccgtca aagtgcgccc cgttatccac gatggcttct ttttccggta catgctgcac
     2041 ggcagtgatg gcatacgtgc cgtcatcgtt atcacggata ctcacacagc ggaacaggcg
     2101 ctgacgcaac gtcggcagct tcagccccca cacactgtat tctgcaacgc cgtcaggaac
     2161 ccggttcact ttcaccttaa gtccgtcggt gacggactga acctccacgc tgaccggatt
     2221 accacttccg tcaaccaggc ttatcagcgt ggtaccggag gatggcagcg tgatttcacg
     2281 gtcgagcgtc agcgtccggg tctggctgtt caccgccagc acgcgaccac cggtgctgat
     2341 accggcatag tcatcatcga agatttcaat aacatcgccc ggtacatggc gaagcccttc
     2401 agcacccacg ctgaaatcca cggtctgcgt ctccagcagt tccgttttaa tcagccacag
     2461 cccggcgcgg tgtgcctgcc cccggctggt acagccaaag gcatccatct tcgtgacatt
     2521 acgaccgtaa cgggcaatgg cctgcgtatc ttcaacaagc tctgtcgccg tctcccagcc
     2581 gttgttcggg tcaatccagt tcacctcaac ggcattatgg cggtccttca gggcgctgaa
     2641 gctgtagcgg aacggcgcgc catcatccgg catcaccaca ttactgcggt tataggtcca
     2701 caccttatct gatggtcggt cctgcacgaa cgtcagcgtc tgcccgttcc ataccggcat
     2761 acagcgcatc gccgagcaga aatcactgag cacatcccac gccttgcgct gtgtggtcag
     2821 gtatgcatta caggtgatgc gcggctccgt gccgccaaag ccgtccggta ctgactggtc
     2881 gcagtactgg ccgatgacat acagcgccca tttgtccaca tccgccgcac caagacgttt
     2941 ccccatgccg tagcgtggat gggtcagcat atcccacaga caccaggcca tgttgttgct
     3001 gtatgccggt ttaaacgttc cgtcccagat accgctgtat tgtcgcgtct gcgggttata
     3061 gttcgacggc acctgcagaa tgcgcccgcg aagatgataa ttacgactca cctgctggct
     3121 gccgaactgc tccaagtcca cctgtacgcc gaccagtgcc gtgttcgggt agcactgttt
     3181 cacatcgatg atttcggtgt atgacgacca gagcgttttg ttctgcagct ggtctgtggt
     3241 gctgtccggc gtcatcctgc gcatccggat attaaacggg cgcgg
//
LOCUS       DAZGLJ010000328         3227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000328 DAZGLJ010000000
VERSION     DAZGLJ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.332"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            190..1710
                     /locus_tag="ACHK49_004763"
     CDS             190..1710
                     /locus_tag="ACHK49_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO9265243.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(1735..2073)
                     /gene="maoP"
                     /locus_tag="ACHK49_004764"
     CDS             complement(1735..2073)
                     /gene="maoP"
                     /locus_tag="ACHK49_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO9265244.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            2192..3031
                     /gene="hdfR"
                     /locus_tag="ACHK49_004765"
     CDS             2192..3031
                     /gene="hdfR"
                     /locus_tag="ACHK49_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO9265245.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(3127..3202)
                     /locus_tag="ACHK49_004766"
     tRNA            complement(3127..3202)
                     /locus_tag="ACHK49_004766"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3167..3169),aa:Trp,seq:cca)
BASE COUNT          865 a          786 c          797 g          779 t
ORIGIN      
        1 tcagtatgaa tgttgttccc ttcctcgtag ttcatccttt gtccgctgca tttttgcgag
       61 catcattcca ccgccagaac aactcaactt cacttgcaca aataaacagg aatcagccac
      121 taaaattcac gaattagcgg gctacagcag cgatatctta tgcacataat agtgactcaa
      181 ggagggctta tgtcactgtc aattgttcat acccgcgcag ccctgggagt aaatgcgccc
      241 ccaatcactg ttgaggtaca tatcagtaaa ggtctacccg gcttaacgat ggtgggctta
      301 ccagaaacaa ctgtaaaaga agctcgcgat cgcgtgcgca gcgccattat caatagcgga
      361 tatgaatatc cggcgaaaaa aatcaccatc aacctggctc cagccgatct gccaaaagaa
      421 gggggacgat atgatttacc tatcgccatt gcgttgctgg cggcctcaga acagcttaca
      481 gccaataagt tagatgaata tgaattagtc ggagaactgg cgcttacagg cgctctgcgt
      541 ggcgttcccg gcgcaatctc cagtgcaact gaagctatta agtcgggcag aaaaattatc
      601 gtcgcgaaag ataacgaaga tgaagtgggg ctaattaacg gtgaaggatg cctgatagcc
      661 gatcatctgc aggctgtctg tgcgtttctg gaaggtaagc acgctctcga acgcccgaaa
      721 ccaactgatg cagtatcccg ggcgctacaa catgatctca gtgatgttgt cggtcaggag
      781 caaggaaagc gaggactgga aattaccgcc gctggcgggc acaacctttt actgattggg
      841 ccgccgggaa caggtaaaac aatgctcgcc agccgtatta atggcctgtt gccagattta
      901 agcaatgaag aggcactgga gagcgctgcg atattaagtc tggtaaatgc tgaatcagta
      961 caaaaacaat ggcggcagcg cccgttccgc tcaccgcatc acagtgcgtc gttaactgcg
     1021 atggtgggcg gtggtgcaat tccagggcca ggtgaaattt cgttggcgca taacggcgtg
     1081 ctttttcttg atgagctacc tgaatttgaa cggcgtacac tggatgcctt gcgagagccg
     1141 attgaatccg ggcagatcca tctttcacgc acacgagcca aaataaccta tccagcccgt
     1201 ttccagcttg ttgcggcgat gaatcccagc cctaccggac attatcaggg aaaccataac
     1261 cgctgcacgc cagaacagac attacgttat ctcaaccggc tctcggggcc ctttctcgac
     1321 cgcttcgatc tctcactgga gatcccatta ccaccccccg gcattttgag taaaacggta
     1381 gtgccgggag aaagcagcgc caccgttaaa caacgcgtaa tggccgccag agagcgccaa
     1441 tttaagcggc agaataaact gaatgcctgg ctggatagtc cggaaatacg ccaattctgc
     1501 aaacttgaga gcgaagatgc gatgtggttg gaaggaacac tgatccatct ggggttatcg
     1561 attcgtgcct ggcagcggtt attgaaagtt gcacgaacca ttgctgatat tgatcagtct
     1621 gacattatca cacgtcagca tttgcaggag gcagttagct atcgtgcgat tgaccgtttg
     1681 ctcatccatc tgcaaaaact actgacataa aaaaagggca tttcgccctt tttattaatc
     1741 atcagaatcg gtgtagtctt cagcaccttc aacctgcggt ttaccgccgg aaagggtgtg
     1801 aaaacgtttt ggacgcttaa tacgcgtcat atacttggac cacacgcgtt ctgcttctgt
     1861 cactggctca cgttcgccac ggcatactgc tacgaagagt ttctcttcct cggtaaccgg
     1921 ctcgcgtttg ccaagatcca actcattgaa ggcataacca tgacgctcaa gcagttgtgc
     1981 ctctttgatg gtgaaatcac catgacgaga gaatccacgt ggataatgtt tattgtcgaa
     2041 atatcgatta gtcgtcgtaa agctttccgc catcctgcac gctcctaatt ctttgaccga
     2101 gctagttatg gcgcggagta ttagttacgc ttgacagagt gtaaaacaaa acatttaaat
     2161 cataacgaca aataattttg cggagagcac tgtggatacg gaattgttaa aaactttcct
     2221 ggaagttagc cgaacgcgtc actttggtcg agcggctgaa tcgctctatc tgacccagtc
     2281 agcagtgagc tttcgaatca gacaactgga aaatcaactg ggtgtgaacc ttttcacccg
     2341 ccacagaaac aatatccgtt taaccgctgc cggtgaaaaa ctactgcctt atgcagaaac
     2401 gctcatgagc acctggcagg ccgcccgcaa ggaggtggcg catacctcac gacataacga
     2461 gttttctatc ggtgccagcg cctcattgtg ggaatgtatg cttaatcagt ggctgggacg
     2521 tttgtatcaa aatcaggatg cccatacagg cttacagttc gaagcgcgaa ttgcccaacg
     2581 gcagtctctg gtaaaacagc tgcatgaacg ccagcttgat cttcttatta ccactgaagc
     2641 gcccaaaatg gacgaattta gtagtcagtt gctgggatat ttcactttag cgctttatac
     2701 cagtgcccct tcaaaactaa agggagatct taattatctg cgacttgagt gggggccaga
     2761 ttttcaacag catgaggcag gtttgatcgg tgctgacgaa gtgcccattc tgacaaccag
     2821 ttctgctgaa ctggcacagc aacagattgc gatgcttaat ggttgcacct ggctacccgt
     2881 cagctgggcg cgtaaaaaag gcggcctgca taccgttgtc gatagcacaa cactttcacg
     2941 gccgctttat gccatatggc tgcaaaatag cgataaaaat acgttgattc gcgatctttt
     3001 gaaaattaac gtgctggatg aagtgtatta atatgaatgg ctggcaagga tgccggtaga
     3061 aggatttact tcggagaggg ttatttcaga taaaaaaaat ccttagcttt cgctaaggat
     3121 gatttctggc aggggcggag agactcgaac tcccaacacc cggttttgga gaccggtgct
     3181 ctaccaattg aactacgccc ctaattaggg tggcggaacg gacggga
//
LOCUS       DAZGLJ010000329         3198 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000329 DAZGLJ010000000
VERSION     DAZGLJ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.333"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..851
                     /locus_tag="ACHK49_004767"
                     /pseudo
     CDS             1..851
                     /locus_tag="ACHK49_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
     gene            complement(891..1183)
                     /locus_tag="ACHK49_004768"
                     /pseudo
     CDS             complement(891..1183)
                     /locus_tag="ACHK49_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(1263..2884)
                     /locus_tag="ACHK49_004769"
                     /pseudo
     CDS             complement(1263..2884)
                     /locus_tag="ACHK49_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          783 a          841 c          762 g          812 t
ORIGIN      
        1 cttattgctg cgctgataat atggaccgca aacctattga agatgtgatc ttcgaaatta
       61 ataactttat ctcgctcggt ggtcgcacca ttgttgatgc cacagggtct gagtctatcg
      121 gacgagatgc gcaggcatta cgagaagtcg cattaaaaac aggtctgaat attgttgctt
      181 cctccggtcc ctacctggaa aaatttgaaa gccagagaat tcataaaacg gttgatgaac
      241 tggcggcaac gattgataaa gaattgaatc aagggattgg cgatacggat attcgtgccg
      301 gaatgatcgg tgaaataggt gtctcaccga catttaccga agccgagcat aacagcttgc
      361 gggctgcctc gctggcacag attaacaatc ctcatgtggc gatgaatatt cacatgccgg
      421 gctggcttcg tcgcggtgat gaagtactcg acattgtgtt aggtgaaatg ggcgtctcgc
      481 caaataaagt ctctctcgca cactcggatc cgtcaggaaa agacgtggcg tatcagcgga
      541 aaatgcttga taagggtgtc tggctggaat tcgacatgat tggcctagac attaccttcc
      601 cgaaagaggg aatagcgcca ggggtgcagg agactgccga tgccgtcgct catctcattg
      661 agttgggata cgccgatcag cttgttctca gccacgatgt cttccttaaa caaatgtagg
      721 ctaaaaatgg cggtaatggc tggggatttg ttccagatgt ttttctggcc tatctggcgg
      781 agcgcggcgt cgataaaacg atcctcaaaa aactctgtat cgataatccc ggacgattat
      841 tgaccgcata aaaccattat cccccggcac actgcgccgg gggatgtcaa ttaatacccc
      901 gccgttaaat catccaccaa gcgcgagtcg gatgcgccgt acaactcacc atccgaccca
      961 accataatgc tttgcgtgct gcccatcgcc tcattcagcg ccactttctg accttttgct
     1021 tccagcagct tgagcgtatc cggactaaac ccttttcaac acgcagctcg tccggcaacc
     1081 actgatggtg gaaacgaggc gcattggtcg cttcggcaac attcattcca caattgatgc
     1141 tgttcaccac catttgcagc acggtagtga tgattcagct accggtaaca aagtgagtac
     1201 ataaaggcca tactgtcacg aacagtgcaa aagtggctct tccgcaacct aggtgaagat
     1261 tttcatgcca gtggactcat taccctccgc ctaagtagtt caaatccggc tcgaccatac
     1321 atctggcgta tcagcacctt caggcgattt acatgacctt cgacaacacc attactccag
     1381 cgactgctga ttgcctcaca tatagccgca gcatcagctt ccatccccgc agctacgcac
     1441 cgaagttctg ctgagccgct ttcgtggact cgagtgaacc agctgcttag ctgtgattta
     1501 ttttaggttt tcagaatacg gtagaactcg agtaccagtt gctgtgctat tttcaactcc
     1561 ggttcttttt cacacatcag actaataaat cgggatgcat aattttcttc gcccctgatt
     1621 attctccagg gcatcaaccc gcggcttacc cggaacactg aaggaagtct tgcggcagta
     1681 gtaaccggtg ggttccagcc tttacgccat ttggcaacag tatccctgac gatggtttca
     1741 ctgcctgtcc ccccccctgg gccaccattt cccgccatat ccggctggca ttataattac
     1801 cactttctcg ttgctctttt aaccactccc tccatgggtc cagaagccct cgctttggag
     1861 gccgggttga catttcaggg aatgttcctg aacgcaccca gcgactgact gtcactcgcg
     1921 acaggcctgt aatacgggat atttccctga aactacatcc cttgttatgc agggccataa
     1981 cctctgtcca atgctgatgc cgttttttgc gggtttgctg tttaaggcgt tccggacgac
     2041 ggagcgatgc tacaggcaca gatatttctg gctcaggtga tttcttcagt gataactctc
     2101 tgacaacaag acgtatcaga ggcatatgtc tgtacatcat tcgttcaggc tcatcaccaa
     2161 tattttttag caggtgccat cgatcggcca cctgtctggc ctgaggtgca ccttcacgtg
     2221 ctgctgtcgc atagactcca ctgcgatcac gcgagacaac ctgtatttcc ggatattttc
     2281 tgaaccaggt cgccagcgta cgctgatcac ggccgggaag caggacgagg ggacggtgag
     2341 tatcaagatt gactattaac ataccgcagc agtggccccg atgccacgcc cactcatcaa
     2401 taccgacatg aggcgcgcct gactgtttcg tctccggggt attgataacc ctgcgaagaa
     2461 gagtatcagc actgcaacgg agacccgctg ccgttgcagc ccgttttcca gcctcacctc
     2521 cagctattaa tcccagttga cgttgtaaat tttgtaacgc ctgtgaagac tgttggtgtg
     2581 aaccggcgaa gggagcaagc gactcggcaa aaattttacg tgaacaagct gggttacggc
     2641 agtaccagtg gcggacggaa aatacaagcc atagcgtctg tcctgatcag ggtagatgct
     2701 gtatccggcg ccgacaacaa ctatgaacag agtcgctacg cttaaagcat tcaggacatt
     2761 gtgctgtttt gcgttttccg tggagatgga gggttatacc gtcagagcta ataatttgtc
     2821 gactgcatcg ccatccgcaa ggtaactgta gaagtgtctt aagagagtgc attgggttgc
     2881 ccataactta aaacagctta gatctttgtt cgccagaaag tttcatccat tttcttccac
     2941 ccaggttgcg gaagaaccac ttttcatccg tcgttgacag atgtgaagta tgcgggagaa
     3001 aaagtgaata tctaactgta ataactcaga ccggtacgag tactcggggc gtctacaatc
     3061 attcagggca aagtgctaaa atttctctat cccgggatgc ccctaccaac gcagcactcc
     3121 cggagatatc attacagctc tgcaactgat ttccgcgact gaaccgctca gttcctatcc
     3181 ttccagttgc cttcctgc
//
LOCUS       DAZGLJ010000330         3179 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000330 DAZGLJ010000000
VERSION     DAZGLJ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.334"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..143)
                     /locus_tag="ACHK49_004770"
     CDS             complement(<1..143)
                     /locus_tag="ACHK49_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFO9265246.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEA"
     gene            complement(873..1070)
                     /locus_tag="ACHK49_004771"
     CDS             complement(873..1070)
                     /locus_tag="ACHK49_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9265247.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(1136..1406)
                     /locus_tag="ACHK49_004772"
                     /pseudo
     CDS             complement(1136..1406)
                     /locus_tag="ACHK49_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1711..1899
                     /locus_tag="ACHK49_004773"
     CDS             1711..1899
                     /locus_tag="ACHK49_004773"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265248.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            2227..2421
                     /locus_tag="ACHK49_004774"
     CDS             2227..2421
                     /locus_tag="ACHK49_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265249.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            2764..2892
                     /locus_tag="ACHK49_004775"
     CDS             2764..2892
                     /locus_tag="ACHK49_004775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265250.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
BASE COUNT          968 a          718 c          699 g          794 t
ORIGIN      
        1 gcttctgctg ctttattcgc gaatttgcca gcgctactaa ttactacatc atgtccaatt
       61 acacgaaagg cctgagccag agaaatcaat gggtataaca aaccgtacag cggtgggcca
      121 ataaaaagaa tacgttttcg cattaaaaaa gtccgtcttc tgcttacctg tttatttttc
      181 tatctctaag aaaacataat taatattatt tttatgtgag cttatgcaca aaaataacca
      241 agtatattat cttctggtag agattcagga tacagtttgt aggagctttc ttttttgaac
      301 aatgatttgc aggacagtta tgctaaacta gcggaatcag tatcacaaca ataaatgact
      361 gatcatacct gaaatttgaa ttaacaggca tcatgaatgt caatagtcta agcggtgact
      421 acttatatcg cttgcataaa tactaaacac tgactagaaa agtattggat aaccccgtca
      481 ctaaaagtaa cgcttcggtt atttgctcgg aatgtattaa cgccaagatt gttagccgtt
      541 acgccttata tcactggcgc tgacaaatgt ttcttttcta atttactgaa ccggcgcaga
      601 cacgcaggaa agcacgccgg caccggtgta atgccagttc taatcaataa gaatggaatg
      661 acgaatcagt caggaaggga taacaacccg ctgctgaacg gaaggtatca gcaggaagat
      721 gaacgggaat atcgccagca ccaagggcag ggccgggttc cacaatacca gcgctggcat
      781 tttgcacatt gccagcgtac cggcattaca acacgtttca tgctgctgcc ccccaggatt
      841 cctcgattcg ttgctacatc cactgctcta cttcacttcc actgctccac ttcacttttg
      901 taccagcggc tgctgcgccc cagtttgatg ggtttaggga aatggccttc actgatcaac
      961 ttgtaaaacc atttgtcggt cataccggtg aaactggtga tatagttcat gtcgatcagg
     1021 gaattatcgg cacgcagaaa tacgctgtcg tggcttgttg tagtcaacat ggtcagaatg
     1081 tcctgtgaga tttatgaaga ttgtgcgaat gcgggaaatc tactgagctg tgctttcaga
     1141 actggcctgt tacgggatac cagggattac cggcggggta acgggcttcc ggatcataca
     1201 caccacgatt atcgcggaca aaatcactga acgcccatat cacctcttta agtatgtctt
     1261 cgcagtccgg taaatgacga tccagcgcca catcccgggt ggtacagctt tgatgcgccc
     1321 ggtgacacaa agcccggatt gttccagaca tcctgaatca aacgccccag attaggggcg
     1381 tcgaaatatg cctctctgac cattatattc cggcgtatag gtagcaggtc tgaagtgaca
     1441 atgcgtcacc tgacgttaaa agtcacccaa gatgacccca gtgggggcaa caagtataaa
     1501 accgtttttt gattaagtgg tcggatatga aaaggggaag gtttatgtga aaaatacctg
     1561 tgttttcttt gaaaacacac aacagtatca ggttatttat gtttatacag tgcgttccaa
     1621 actgacctga ataattacgc aagttgcaac aataaaacat ttttgccgta gtgtgaggta
     1681 ctccggtaaa ttgcttccca ggtgcagaca atgactaacg aagaactaca ggcaatcgca
     1741 ttatccatgc ttgagcgcca gcgcgaaaaa gtacaccagc aacgagagtc tttgcttaac
     1801 ccatctccat tcaccaggcc aaaacgattg gagcttgaag cattcttgga caaataccca
     1861 cggcggctta aatcaggcaa aaccagacca ccaggctgat agctcacagt ttgttctccg
     1921 attcaataaa acggataaga accatcagcg cggtagcgta aggcactaca gcgcattgct
     1981 ttacgagtaa taacaatgga tttatcacca gaagacgcaa agtttttacg agaactgcaa
     2041 caccgtatgc tcagagaagc cattatcgaa cagcaaacgg gcatgagcgc catagaccca
     2101 ttgcgataca aacgggcctc atctccttta gttgacgaca attccaatgc ggataaaaca
     2161 cgtccaccac actgattaca tttttcatta gcacagtggt gtgatgcagc atacgaggaa
     2221 aaccggatga atgagaacaa aataaaaagg ctggagcaac tgttacaggc acgccaacgg
     2281 gagtttgcca ctaaatgggg acgtgccgcg ctgccacatg aacgcctaac ctattttcca
     2341 cttgaagacc tcattaaaaa taatggcaaa aaaatcaggt ctgataagaa acacttctta
     2401 gaatgcaatg aagacaaata acaacaatct gcatattgcc accaaccaca gatgtggcat
     2461 cgttatcagc gcacttgatt ttctttatat ccactgacga gtttgccggt cattctggta
     2521 tcatgcagat atttacggcg tacaagcaca ctagcatgac attgtaataa ggcctgagcc
     2581 taacgcttta atgtgtgtca tacactcagt ggcgtaatcg ttgacaatat ggtgcatgta
     2641 atcgtaagcg tccagcgaaa tccatctggt cataatctga agcatccgac aaagtggtgt
     2701 ccaccaaata agtgatctgc gcccaatatc ctgagccgga actttgacaa aggccaacga
     2761 aaaatgggag cggatgttac agagttctcg gtacagggga aaagtggtac ctgtcgccag
     2821 ttctccgatc tgtttaaccg ggaaattatc tcctacagct tgtcagaaag gtcggtgatg
     2881 gagcatcgtt aatacgatgc tatacgatgc attcacagtg cccggccaga ggatgccccg
     2941 tcgctgcata tggatcaggg ttggcaatat cgaattgcag gctataggca aagttaaagc
     3001 cccatggagt agcacaaaat atgccgcaca aaggaaacgg tctgaataac gcagtgatga
     3061 agaacttctt cagcacactg ctaaaacgca tagtgatcga gcgctgattc tggcgaacaa
     3121 ctgaactaat acatcagaat ctgcattatg ttaaataaat ataaaaagat ggtttaaat
//
LOCUS       DAZGLJ010000331         3173 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000331 DAZGLJ010000000
VERSION     DAZGLJ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.335"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(2..604)
                     /gene="fimB"
                     /locus_tag="ACHK49_004776"
     CDS             complement(2..604)
                     /gene="fimB"
                     /locus_tag="ACHK49_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO9265251.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            2060..2776
                     /gene="nanC"
                     /locus_tag="ACHK49_004777"
     CDS             2060..2776
                     /gene="nanC"
                     /locus_tag="ACHK49_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO9265252.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVDVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2796..>3173
                     /gene="nanM"
                     /locus_tag="ACHK49_004778"
     CDS             2796..>3173
                     /gene="nanM"
                     /locus_tag="ACHK49_004778"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO9265253.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWKALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPM"
BASE COUNT          992 a          642 c          630 g          909 t
ORIGIN      
        1 tctataaaac agcgtgacgc tgtcgtcctc tggctctatc ccagatgccg taaaaacgcc
       61 ctgcattgct ggcggtatac cagacagtat gacgaatatt gcgatgccca agataatcct
      121 ggataagtcg cgtatctatt cccatattcg ccaaagcaaa accacacgaa tggcgtaaca
      181 tgtgcggatg aatctccagt gacaacccgg cattaccacc ggaagtcgag ataatatggt
      241 aaaactgttg ccgagaaagc ggatttccct tacgcgataa aaatacccat tcgctctcag
      301 catgcgggta cgaagtacgg atactcaacc agttttttaa agcctgaact tctttgttca
      361 atagcgggtg cgttgttgaa aagccttttt ttaatcgatg gatatatata cactttgcct
      421 taagatcaat atccgaaatc ctcaatcgac aaatttcact cgcccggaaa ccatgaataa
      481 aacaaagcaa agtcagacaa taattacgtg ctgcatgagg cccggtattt gctgctttaa
      541 ggagtgattc gatttcacta tgggtcagga agttcctttt tttgttatca gccttattct
      601 tcatcgtttt tcccttataa ttacagacgc gcactagctg tgctgggtta gcaataatcc
      661 aacattttta tcctgattat gtttataaaa gcgaacgttt gcttaataac taacgtaagt
      721 ggaccagttc ttctgagtga acttaaatgg agtagcaaca gttacttata ataacgttgc
      781 catacaaacg ctatgctctt gcatgctatg tacctttata tatttatcaa tcggatacga
      841 atctaataat aaccctcatc attaatgcaa ctaatcttat ctatatatca tgtgatatgt
      901 tctgtaacaa gtaaaatcaa cataaaaaat tcagcaaaat ttaatattcg ttcaactatc
      961 cgtctcacat atgacattat tagacaactc catttgccac aaaatggcta tttcatggaa
     1021 cgcagtaatt tcttagtagc tattatcagg ctaattctta ggtccctagc gattattcct
     1081 ggctggttcg tcagtttagt tcatgagatt gttacattcc tttttatcct ggagatatgg
     1141 gggaaagcag gagatgattt tttcatctgc atccgttggt aaatatggct ttggtcactg
     1201 cgacttaaac cttcctcaat cacagctgtt cgccgggcta ttcggcaaac aagctaagtg
     1261 aaacatcatc ctgaaaacac caacatcaac aagcctctcc agagagactt caggagtgac
     1321 cagctacaag ccacaaacca ggaaacatat ttcatgatga gaattatgct cagcctgatg
     1381 gcgggtaaac acttattttc accacgccca ctgagttatc ttgcaaatct ttccttgttt
     1441 atactctaaa taaattactt ataaaacaga taattaaata acaacccttg gtttgtgaag
     1501 gcctccgcaa gtgttcgttt ggcttcacat ggcatcttct tcttagaaaa atatcgacat
     1561 attttgtgac acgaattgca aatctggttt tgttgtatgg attgcgtgat ttttgatctg
     1621 gtataacagg tataaaggtg caccaagata gtcaatgaga cagggcatct cgcaatctat
     1681 ggcaaacatc acttcagttc tttcacatcg ggtgatgaaa atgcacttca gtctgaaagg
     1741 aatatgaaaa tgagatcaac agacactcta ttttatgact ctgggtaaaa tggattgagt
     1801 aagtgatata gcttacgaac attcaaatca attaaacatc agaagagatt ttatactcag
     1861 gtatttaatc tggctctctg tttatttaaa taatgtgaaa agagattttt cacaggagac
     1921 cttatacaaa aaatataaaa tacagctacc ggttgccaaa gacactataa gcctggcaaa
     1981 aaaatattac acaacataaa tgctaattgt ttatgcgggc tttgtattgc tttctgtttc
     2041 ctacaaatga gtgaaattta tgaaaaaggc taaaatactt tctggcgtat tattactgtg
     2101 cttttcgtca ccattaattt ctcaagctgc gacactggac gtacgtggtg gatatcgtag
     2161 tggaagccac gcctatgaga ctcgactcaa agtcagtgag ggatggcaaa atggatggtg
     2221 ggcaagtatg gaaagtaata cctggaatac cattcatgat aataaaaagg aaaatgccgc
     2281 actcaatgat gttcaggttg atgttaatta cgcgattaaa cttgatgatc aatggacggt
     2341 gcgcccggga atgttaacgc attttagcag caacggcaca cgctacggac cctacgtaaa
     2401 actgtcctgg gacgcgacaa aagatcttaa ttttggcatt cgctatcgtt acgactggaa
     2461 agcttaccga caacaagact tatccggtga tatgtctcgt gataacgttc atcgttggga
     2521 tggatatgtc acttaccata ttaatagtga tttcaccttc gcatggcaaa cgacgctata
     2581 cagcaaacag aacgattatc gctatgcaaa ccataagaaa tgggcgacgg aaaatgcatt
     2641 tgttctacaa taccatatga cgccggatat tacgccatac atagaatatg actaccttga
     2701 ccgtcagggt gtttacaacg gcagagataa tttatcggaa aacagttatc gcattggtgt
     2761 gtcatttaaa ctgtagtaga caggagacag tcacaatgaa taaaacaata acggcgcttg
     2821 ctatcatgat ggcttcattt gccgcaaacg cgtctgtatt accggaaact cctgtgccat
     2881 ttaaaagtgg taccggagca attgataacg acactgtcta cattggttta ggtagcgcag
     2941 gtacggcatg gtacaagctg gatacacagg ccaaagataa aaaatggaaa gcgttagctg
     3001 cattccctgg tggaccaaga gatcaagcaa cctcggcatt tattgatggc aatctgtatg
     3061 tgtttggcgg cattggcaaa aacagcgagg gcttgactca ggtatttaat gacgtacaca
     3121 aatacaaccc caaaaccaat agttgggtta aattgatgtc gcacgcaccg atg
//
LOCUS       DAZGLJ010000332         3161 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000332 DAZGLJ010000000
VERSION     DAZGLJ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.336"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..2407
                     /locus_tag="ACHK49_004779"
     CDS             <1..2407
                     /locus_tag="ACHK49_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO9265254.1"
                     /translation="VEFNKDLIEAEDRENVNLSQFETDGQLPVGKYSLNALINNKRTP
                     IHLDLQWVLIDNQTAVCLTPEQLTLLGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLD
                     KGKMQLSISAPQAWLKYKDANWTPPELWDHGIAGAFLDYNLYASHYAPHQGDNSQNIS
                     SYGQAGVNLGAWRLRTDYQYDQSFNNGKSQANNLDFPRIYLFRPIPAINAKLTIGQYD
                     TESSIFDSFHFSGVSLKSDENMLPPDLRGYAPQITGVAQTNAKVTVSQNNRIIYQENV
                     PPGPFAITNLFNTLQGQLDVKVEEEDGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPT
                     SVGGDSLQQPFFWTGEFSWGWLNNVSLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFD
                     VTRSDAQLHNQDKETGYSYRANYSKRFESTGSQLTFAGYRFSDKNFVTMNEYINDTNH
                     YTNYQNEKESYIVTFNQYLESLRLNTYVSLARNTYWDASSNVNYSLSLSRDFDIGPLK
                     NVSTSLTFSRINWEEDNQDQLYLNISIPWGTSRTLSYGMQRNQDNKISHTASWYDSSD
                     RNNSWSVSASGDNDEFKDMKASLRASYQHNTENGRLYLSGTSQRDSYYSLNASWNGSF
                     TATRHGAAFHDYSGSADSRFMIDADGAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSL
                     SVDTRNLPENVDIENSVITTTLTEGAIGYAKLDTRKGYQIMGVIRLADGSHPPLGISV
                     KDKTSHKELGLVADGGFVYLNGIQDDSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCI
                     SQN"
     gene            2423..>3161
                     /locus_tag="ACHK49_004780"
     CDS             2423..>3161
                     /locus_tag="ACHK49_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9265255.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAK"
BASE COUNT          985 a          669 c          641 g          866 t
ORIGIN      
        1 cgttgaattc aacaaagatt taatcgaagc cgaagatcgt gaaaacgtta acctttccca
       61 atttgaaact gatggccaat tacccgtagg taaatactca ttaaacgctc ttattaataa
      121 taaaaggacg ccaatccatc tggacctgca atgggtatta attgataacc aaactgcagt
      181 ttgtctgact ccagagcaat taacattatt aggctttact gatgagatta ttgaagaggc
      241 tcagcaaaac ctgatcgatg gttgttaccc tatcgaaaaa gaaaaacaaa ttacaactta
      301 tctcgataaa gggaaaatgc aattatccat atctgcacct caggcatggt taaaatacaa
      361 agatgcaaac tggacgcctc ctgaactttg ggatcatggt attgctgggg catttcttga
      421 ctacaattta tatgcctctc attatgcacc acatcagggc gataattcac aaaatataag
      481 ttcctatggg caggctgggg ttaatcttgg ggcctggcgc ctgcgtactg attaccagta
      541 cgatcagtca tttaacaatg gcaaaagcca ggcgaacaac ctggattttc cgcgtattta
      601 tttgtttcgc ccaatcccag caattaatgc aaaactaact ataggtcaat acgatactga
      661 atcctctatt ttcgactctt tccatttttc tggcgtttcg ttgaaaagcg atgagaatat
      721 gttgccgcct gatctacgtg gttacgcacc gcaaatcacg ggtgttgcac aaacgaatgc
      781 aaaggtcact gtctcacaga acaaccgtat tatttatcaa gaaaatgttc ctcctggccc
      841 atttgctatt actaatttat tcaatacatt acaggggcaa ctggacgtca aggttgaaga
      901 agaggacggg caagtcacgc aatggcaagt tgcatctaat agtattcctt atctgacgcg
      961 taaagggcag attcgctaca ccactgctat gggtaaaccg accagcgttg gtggtgattc
     1021 cttacaacaa cccttcttct ggactggtga attctcatgg ggttggctga acaatgtatc
     1081 cctgtatggt ggttcagttt taacaaaccg tgattatcaa tctctggctg ccggcgttgg
     1141 ctttaacctt aactcattgg gttcattatc ttttgatgtc acacgatctg atgctcagtt
     1201 gcataatcag gataaagaaa cgggttatag ctaccgcgct aactattcaa aacgttttga
     1261 atctaccggt agccagctca ctttcgctgg ttaccgtttc tctgataaaa actttgtgac
     1321 aatgaatgaa tatatcaatg acactaacca ttacacgaat tatcagaatg aaaaagagag
     1381 ttatattgtc acgtttaacc agtatcttga atcattaagg ttaaatacat acgtaagttt
     1441 ggctcgtaat acttactggg acgccagcag taatgtgaat tattcattat cacttagccg
     1501 cgattttgat atcgggccat taaaaaacgt ctccacttct ctaacattta gccgaataaa
     1561 ctgggaagaa gacaaccagg atcaactgta cctaaatatt tcgattccct ggggaaccag
     1621 tagaacattg agctatggta tgcaacgaaa tcaggataac aagatttcgc atactgcttc
     1681 gtggtatgac tcttccgatc gaaataattc ctggagcgtt tctgcttcag gcgacaatga
     1741 tgaattcaaa gatatgaaag cgtcactacg cgccagttat cagcataata ccgagaacgg
     1801 tcgtctctat ctctccggga catcacagcg agacagttat tattctctga atgccagttg
     1861 gaatggttca ttcacggcga ctcgtcacgg agccgctttc catgactata gtggtagtgc
     1921 tgactcgcgt tttatgatcg acgcagatgg tgcagaagat attccgttga acaataaacg
     1981 cgcggtaact aatcggtatg gcatcggagt tattccatca gtcagcagtt acataacaac
     2041 atcattaagc gttgataccc gaaatcttcc agaaaatgtg gatatcgaaa actcggttat
     2101 caccaccacc ttaaccgaag gtgctattgg ctacgccaaa cttgataccc gcaagggcta
     2161 tcaaatcatg ggggttattc gcctggcaga tggtagtcac ccaccactgg ggattagcgt
     2221 aaaagataaa accagccata aagaattagg actggttgct gatggcggct ttgtatacct
     2281 caacggcatt caggatgaca gcaaacttac tttacgctgg ggtgataaat cctgttttat
     2341 tcaaccaccc aatagcagca acttaaccac cggaacggtt attttaccgt gtattagcca
     2401 aaattaattc aggatgaaac taatgcgcta cttaaacaca aagaatctca ttgcagcagg
     2461 agtattactc gcctgcatga gcagcattgc ctggggtgcc atcatcccgg accgtacccg
     2521 tatcataatg aatgagtcag ataaagggga agcattaaaa cttactaatc aaagcaaaaa
     2581 tctaccctat ctggcgcaaa catggataga agatacgaaa ggcaataaat cacgtgactt
     2641 tattgtcacg gttcccccta tggtacgttt aaatcccagt gagcaaattc aaataagaat
     2701 gataagtcag gaaaaaattg ctcaactgcc taatgacaga gaaacgctct tctattttaa
     2761 cgtgcgagaa ataccgccaa aaacagataa aaaaaatgtg atgcaagtca ctatgcagca
     2821 cgcattgaaa ctattctggc gaccaaaagc tattgaatta gaagatgatg gggtaatgtc
     2881 ttacgaaaaa gttgaaatca tccgtagaaa tgatggttcg atccgcttta acaataagat
     2941 gccttatcac gttacgctgg gttatatcgg aacaaacgga atcacaatgt tgccacaaac
     3001 gcaaagttta atggtaaccc cttatagcca tgcagatacg cagtttaaaa atgttccgtc
     3061 agcttttcag gttggttata tcaatgattt tggtggatta agtttttatg aaataaactg
     3121 cccgacggtt aataactctt gtaatgtttc cgtagccaaa c
//
LOCUS       DAZGLJ010000333         3135 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000333 DAZGLJ010000000
VERSION     DAZGLJ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.337"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..461
                     /locus_tag="ACHK49_004781"
     CDS             <1..461
                     /locus_tag="ACHK49_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO9265256.1"
                     /translation="ILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKT
                     EAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRG
                     QAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDSVVSVARAVWNWIFF"
     gene            830..>3135
                     /locus_tag="ACHK49_004782"
     CDS             830..>3135
                     /locus_tag="ACHK49_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag43/Cah family autotransporter adhesin"
                     /protein_id="HFO9265257.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNGGTLTNHDNQIVFGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATVTVINEKGWQAVKSGAMATDTVVNTGAEGGPDAENGDTGQNVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATDVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIHFDNQTTQDAVLSRAVAKGDSPVTFHK
                     LTTTNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATTGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYAS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGGYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     VHTSSGLWADIVAQGTRHSMKASSDNND"
BASE COUNT          733 a          816 c          943 g          643 t
ORIGIN      
        1 acatcctgca ccggggacat cagcaggtgc tgtttgtggt gacgcaggcc gacaaaacgg
       61 agccctgcca tgaatgggat atggccggta ttcagccttc tcccgcacag gcacagaata
      121 ttcgcgaaaa aacggaggcg gtattccgtc tgttccggcc cgtacatccg gttgtggccg
      181 tatcggcccg caccggctgg gaactggata cgctggtcag tgcgctcatg acagcgcttc
      241 ccgaccatgc cgccagtccc ctgatgaccc gcctgcagga cgagctgcgc acggaatctg
      301 tccggggtca ggcccgtgaa cagtttaccg gtgcggtgga ccggatattt gacacggcgg
      361 agagcgtctg tattgcctct gttgcacgca cggttctgcg ggccgtccgt gactcggtgg
      421 tctctgttgc ccgcgcggta tggaactgga ttttcttctg aacactctgc caggggaaat
      481 gcctgattac attgcgttaa ccaactgaaa acatgccccc ccctcatacc cacctctttc
      541 tcttgcgtga cttccctgct gttaatagaa taaaacgatc gataaaacag gtatcgatcg
      601 ttactatcga tcgtttatag tggttaatgg tctgcgaaaa ggcagctgaa tctcttcatc
      661 atgcagaacg gaattgcaca caacggactg atacttctct ggcggatgag agaggggaaa
      721 catttatgtc cgggaaaaac cactttatct gaccatgctg ttttgtacct gccggtatcc
      781 acttttgtgg gtaccggctt ttttattcac cctctgtaag gaaaagctga tgaaacgaca
      841 tctgaacacc agctacaggc tggtatggaa tcacattacg ggcaccctgg tggtggcctc
      901 cgaactggcc cgctcacggg gaaaacgcgc cggtgtggcg gttgcgctgt ctcttgctgc
      961 tgtcacatca gtcccggcac tggctgctga cacggttgta caggcgggag aaaccgtgaa
     1021 cggcggaaca ctgacaaatc atgacaacca gattgtcttc ggtacggcca acggaatgac
     1081 catcagtacc ggtctggagt atgggccgga taacgaggcc aataccggcg gacaatggat
     1141 acaaaatggc ggtatcgcca acaacactac tgtcaccggt ggtggtcttc agagagtgaa
     1201 tgccggagga agcgtttcag acacggttat cagtgccgga ggcggacaga gccttcaggg
     1261 gcaggcagtg aacaccactc tgaacggcgg tgagcagtgg gtacatgaag gcgggattgc
     1321 aacggttacc gtcattaatg agaagggctg gcaggccgtc aaatccggcg caatggcaac
     1381 cgacacggtt gtgaataccg gcgcggaagg gggaccggat gcagaaaatg gtgataccgg
     1441 gcagaacgtc tacggagatg ccgtacgcac caccatcaat aaaaatggtc gtcagattgt
     1501 ggctgctgaa ggaacggcaa ataccactgt ggtttatgcc ggcggcgacc agactgtaca
     1561 tggtcacgca ctggatacca cgctgaatgg ggggtaccag tatgtgcaca acggcggtac
     1621 ggcgtctggc actgttgtaa acagtgacgg ctggcagatt atcaaggaag gtggtctggc
     1681 ggatttcacc accgttaacc agaaaggcaa actgcaggtg aacgccggtg gtaccgccac
     1741 acatgtcacc ctgaaacagg gtggagcact ggtcaccagt actgcggcaa cggttcttgg
     1801 cagcaaccgt ctgggcaatt tcacggtgga aaacggtaag gctgacggcg ttgttctgga
     1861 gtccggcggt cgcctggatg tactggaagg ccattcagcg cagaaaacac gggtggatga
     1921 cggcggtacc ctggcagtgt ctgccggtgg taaggcaaca gatgtcacca tgacatccgg
     1981 tggtgccctg atagcagaca gtggtgccac tgttgagggg accaatgcca gcggtaagtt
     2041 cagtattgat ggcatatccg gtcaggccag cggcctgcta ctggaaaatg gcggcagctt
     2101 tacggttaat gccgggggac aggcaggcaa caccactgtc ggacatcgtg gaacactgac
     2161 gctggctgcc gggggaagtc tgagtggcag aacacagctc agtaaaggcg ccagtatggt
     2221 actgaatggt gatgtggtca gtaccggcga tattgttaac gcaggagaga ttcactttga
     2281 taatcagacg acacaggatg ccgtgctgag ccgtgctgtt gcaaaaggcg actccccggt
     2341 aacgttccat aaactgacca ccaccaacct cactggtcag ggcggcacca tcaatatgcg
     2401 tgttcgcctt gatggcagta atacctcaga ccagctggtg attaatggtg gtcaggcaac
     2461 cggcaaaacc tggcttgcgt tcacaaatgt cggaaacagt aacctcgggg tggcaaccac
     2521 cggacagggt atccgggttg tggatgcaca gaatggtgcc accacagaag aaggtgcgtt
     2581 tgccctgagc cgcccgcttc aggccggcgc ctttaactac accctgaacc gtgacagcga
     2641 tgaagactgg tacctgcgca gtgaaaatgc ttatcgtgct gaagtccccc tgtatgcctc
     2701 catgctgaca caggcaatgg actatgaccg gattctggct ggctcccgca gccatcagac
     2761 cggtgtaaac ggtgaaaata acagcgtccg tctcagcatt cagggcggtc atctcgggca
     2821 cgataacaac ggcggtattg cccgtggggc cacgccggaa agcagcggca gctatggctt
     2881 cgtccgtctg gagggtgacc tgctcagaac agaggttgcc ggtatgtctc tgacgacagg
     2941 ggggtatggt gctgcaggcc attcttccgt tgatgttaag gatgatgacg gctcccgcgc
     3001 cggcacggtc cgggatgatg ccggcagtct gggcggatac ctgaatctgg tacacacatc
     3061 ctccggcctg tgggctgaca ttgtggcaca gggaacccgc cacagcatga aagcatcatc
     3121 ggacaataac gactt
//
LOCUS       DAZGLJ010000334         3135 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000334 DAZGLJ010000000
VERSION     DAZGLJ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.338"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            98..1780
                     /gene="ydeN"
                     /locus_tag="ACHK49_004783"
     CDS             98..1780
                     /gene="ydeN"
                     /locus_tag="ACHK49_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO9265258.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1832..2989
                     /gene="ydeM"
                     /locus_tag="ACHK49_004784"
     CDS             1832..2989
                     /gene="ydeM"
                     /locus_tag="ACHK49_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO9265259.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKNLFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2955..3077)
                     /locus_tag="ACHK49_004785"
     CDS             complement(2955..3077)
                     /locus_tag="ACHK49_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265260.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKGRVINYSPFVLQHP"
BASE COUNT          950 a          680 c          633 g          872 t
ORIGIN      
        1 atctgctatc tctttatgtg actaacttca cttacatcca cttatttctc ttcgtaaaat
       61 tacttcggaa ttaagtacaa taagaagagg aacatttatg aagtctgcat taaagaaaag
      121 tgtcgtaagt acctcgatat ctttgatact ggcatctggt atggctgcat ttgctgctca
      181 tgcggcagat gatgtaaagc tgaaagcaac caaaacaaac gttgctttct cagactttac
      241 gccgactgaa tacagtacca aaggaaagcc aaatattatc gtactaacca tggatgatct
      301 tggttatgga caacttcctt ttgataaggg atcttttgac ccaaaaacaa tggaaaatcg
      361 tgaagttgtc gatacctaca aaatagggat agataaagcc attgaagctg cacaaaaatc
      421 aacgccgacg ctcctttcat taatggatga aggcgtacgg tttactaacg gctatgtggc
      481 acacggtgtt tccggcccct cccgtgccgc aataatgacc ggtcgagctc ccgcccgctt
      541 tggtgtctat tccaataccg atgctcagga tggtattccg ctaacagaaa ctttcttgcc
      601 tgaattattc cagaatcatg gttattacac tgcagcagta ggtaaatggc acttgtcaaa
      661 aatcagtaat gtgccggtac cggaagataa acaaacgcgt gactatcatg acaacttcac
      721 cacattttct gcggaagaat ggcaacctca aaaccgtggc tttgattact ttatgggatt
      781 ccacgctgca ggaacggcat attacaactc cccttcactg ttcaaaaatc gtgaacgtgt
      841 ccccgcaaaa ggttatatca gcgatcagtt aaccgatgag gcaattggcg ttgttgatcg
      901 tgccaaaaca cttgaccagc cttttatgct ttacctggct tataatgctc cgcacctgcc
      961 aaatgataat cctgcaccgg atcaatatca gaagcaattt aataccggta gtcaaacagc
     1021 agataactac tacgcttccg tttattctgt tgatcagggt gtaaaacgca ttctcgaaca
     1081 actgaagaaa aacggacagt atgacaatac aattattctc tttacctccg ataatggtgc
     1141 ggttatcgat ggtcctctgc cgctgaacgg ggcgcaaaaa ggctataaga gtcagaccta
     1201 tcctggcggt actcacaccc caatgtttat gtggtggaaa ggaaaacttc aacccggtaa
     1261 ttatgacaag ctgatttccg caatggattt ctacccgaca gctcttgatg cagccgatat
     1321 cagcattcca aaagacctta agctggatgg cgtttccttg ctgccctggt tgcaagataa
     1381 gaaacaaggc gagccacata aaaatctgac ctggataacc tcttattctc actggtttga
     1441 cgaggaaaat attccattct gggataatta ccacaaattt gttcgccatc agtcagacga
     1501 ttacccgcat aaccccaaca ctgaggactt aagccaattc tcttatacgg tgagaaataa
     1561 cgattattcg cttgtctata cagtagaaaa caatcagtta ggtctctaca aactgacgga
     1621 tctacagcaa aaagataacc ttgccgccgc caatccgcag gtcgttaaag agatgcaagg
     1681 cgtggtaaga gagtttatcg acagcagcca gccaccgctt agcgaagtaa atcaggagaa
     1741 gtttaacaat atcaagaaag cactaagcga agcgaaataa ctaaaccttc atgcggcgga
     1801 attttccgcc gccttattga gcgagatagc gatgcacgtt acagccaagc cctccagttt
     1861 tcaatgtaat ctcaaatgtg attactgttt ttaccttgaa aaagagtcgc agtttactca
     1921 tgaaaaatgg atggatgaca gcactctgaa agagttcatc aaacaatata tcgcagcgtc
     1981 tggcaatcag gtctatttta cctggcaagg cggtgaaccc actctggctg gcctggattt
     2041 tttccgtaaa gttattcact atcaacaacg ctatgcaggc caaaaacgta tttttaatgc
     2101 attacaaacg aatggcattt tattgaataa tgaatggtgt gcttttctca aagaacatga
     2161 gtttctggtt ggtatctcga tcgatggccc ccaggagcta catgaccgtt acagacgcag
     2221 taattcaggt aacggtactt ttgcaaaagt gatagcagcc atcgagcgtc tgaaatcata
     2281 tcaagttgag tttaatacgt taaccgtcat taataacgtt aatgtccatt accctcttga
     2341 ggtttatcat tttttaaaat ctatcggcag taaacatatg caatttatcg aattgctcga
     2401 aaccgggacg ccgaatattg atttcagtgg tcatagtgag aacacattcc gtatcattga
     2461 tttttctgtg cctcccacgg cttatggcaa gtttatgtca accattttta tgcaatgggt
     2521 taaaaacgat gtgggtgaaa ttttcattcg tcagtttgaa agctttgtca gccgtttttt
     2581 ggggaatggg cataccagtt gtattttcca ggagtcctgc aaggataatc tggttgttga
     2641 aagtaatgga gacatttacg aatgcgacca ttttgtctat ccacagtaca aaattggaaa
     2701 cattaataaa tctgaactca aaacgatgaa cagtgtacaa ctgacagcgc aaaaaaaacg
     2761 gatttcagcg aaatgtcagc aatgtgcata taaacctatc tgcaatggcg gttgtcctaa
     2821 gcatcgtatt actaaagtaa acaatgagac tgtttcttat ttttgcgaag gttataaaaa
     2881 ccttttttca accatggtac cttatatgaa cgccatggtg gagttagcta agaacagagt
     2941 accgctttat cacattatgg atgttgcaaa acaaatggag aataattaat aacacgccct
     3001 tttagctcca ttcttagcct ccttcttatg accttaacaa ggcacccgac ataaattcct
     3061 gtcgggtgtt tcgtcatcat catcttccct tgcgaaaatc ctggcttacg tggtatgcgt
     3121 cagggcatta gcaat
//
LOCUS       DAZGLJ010000335         3134 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000335 DAZGLJ010000000
VERSION     DAZGLJ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.339"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..77
                     /locus_tag="ACHK49_004786"
     CDS             <1..77
                     /locus_tag="ACHK49_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265261.1"
                     /translation="DPNYCWREVDPVDAANEAMSYHND"
     gene            110..327
                     /locus_tag="ACHK49_004787"
                     /pseudo
     CDS             110..327
                     /locus_tag="ACHK49_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
     gene            329..592
                     /locus_tag="ACHK49_004788"
     CDS             329..592
                     /locus_tag="ACHK49_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265262.1"
                     /translation="MATLTKKERAWLNELQEVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            603..728
                     /locus_tag="ACHK49_004789"
                     /pseudo
     CDS             603..728
                     /locus_tag="ACHK49_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1284..1472
                     /locus_tag="ACHK49_004790"
     CDS             1284..1472
                     /locus_tag="ACHK49_004790"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO9265263.1"
                     /translation="MPDTKTAVLVARDFGRKGDWRMKWATYIPGHPDANDGWIIPGAS
                     WIPSHWMPLPEPPQEVRQ"
     gene            1588..1692
                     /locus_tag="ACHK49_004791"
     CDS             1588..1692
                     /locus_tag="ACHK49_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265264.1"
                     /translation="MSDDISLAMEGALAVVAVVGVYCLVVFLMDRLGN"
     gene            1939..2094
                     /gene="hokD"
                     /locus_tag="ACHK49_004792"
     CDS             1939..2094
                     /gene="hokD"
                     /locus_tag="ACHK49_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO9265265.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            2262..2540
                     /locus_tag="ACHK49_004793"
     CDS             2262..2540
                     /locus_tag="ACHK49_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265266.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSRTFKSDWFQHPPCTEEQAEW
                     LIQCYRRHGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            2542..>3134
                     /locus_tag="ACHK49_004794"
     CDS             2542..>3134
                     /locus_tag="ACHK49_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9265267.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEHLAQQNLAAWMIDVIRHAMNGSQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRS"
BASE COUNT          827 a          729 c          832 g          746 t
ORIGIN      
        1 ccgacccaaa ttattgctgg agggaggttg atccagttga tgctgcaaat gaagctatga
       61 gttatcacaa cgattaatgt caggaggccg cccgaaaggg cggtaatgaa tggtcacatt
      121 atttagaaaa aatatccgcg aaagagtaga acaacagaat ttctgtttct cattctgttt
      181 atcgtgttga tgataccgat atccccgtta atcctagtct ggataatcgg aaaaataatt
      241 gagccagtta ttgaattgta taacgacgtg gtatgggcgt cattcaacac actgcacaat
      301 aaaattaatc cgtataagga aaactgatat ggcaactttg acaaaaaaag aacgggcatg
      361 gttgaacgaa ttacaggaag ttcttgatcg ctgtccatca ccgaaaaaaa ttggctttta
      421 caccattggc gataaaagca tttacctgta tgacctacgc cgcatggatg aaatcatgga
      481 ggctcttgat aatcgttcgt cgatggattg gtgtgttgct gttcatgata tgaatgcagg
      541 gtttgatgaa aagattttgt tcccctcatc agttgaaagc actgcgggtt aaggagtaac
      601 acatgaccac tattaccaaa gaacgtattg aattgttcat taaaaatccg cttgaaaacg
      661 ggcttacccg tggtgaacaa atggaactgg cacggattgc gctggcatcg ctggaagcag
      721 agccggtggc aaagattata gctcattacc cattaggggt tgacgtaggc aaacaaaaat
      781 ttgtacaggc cattagagag cttcctgact ttggcggata tctatttgcc gcccctccag
      841 cgccgatagt gccggaagaa atgtattggc aggatgcgcc agttgaaggc agcagcaaag
      901 cggctgcata cgctacaggc tggaacgatt gccgcgaagc catgtttcag tccggaaact
      961 ttcgggaaaa taaagattcg tcaaccaata attttcggaa aatcccggaa gcgtcaacca
     1021 gctctccggt aactccggct cttctgcctg gtggtttcac cattgaggag gcgaaggaat
     1081 tacatgaaga cctggcacgc agccacataa gcaaggcctt aagtggcgaa aagatgaaaa
     1141 agaaagatcg cgatgctgat ttgcgctgga ttcatggcgt tatagttcag gcagcgtggt
     1201 ttgtaaaagc atcactggag cagaatgcac tatcgggcaa ctatccggta actccggata
     1261 gttggataag ctgtagtgag cgaatgccgg ataccaaaac agccgttctt gttgccaggg
     1321 attttggcag gaaaggtgac tggcgaatga aatgggcgac ttacatcccg gggcatcctg
     1381 acgctaatga tgggtggata atacctggtg cgtcgtggat accatcacac tggatgcctc
     1441 taccagaacc gccgcaggag gtgcgccaat gaactggcct gaagcatttg caattacagg
     1501 cgttgctatg gctatcgctt ttttagtata tgttatttgt cggtgagggt aaaaacgttc
     1561 gccgggatta acaccaaagg agggaatatg tcggatgata tatcactggc aatggaaggt
     1621 gcgctggctg ttgttgctgt tgtgggcgtt tactgcctgg ttgtgttttt gatggatcga
     1681 ctagggaact gaattcatta cgatatggga attcccatat cgggtaaaaa cggtttgcgg
     1741 gaaaaggaga gttaagtaga attgctgcgg gtgcttgagg ctatctgtct caggcatgaa
     1801 caccaaaagg cagatagaga aaagccccag ttaacattac gcgtcctgca agacgcttaa
     1861 cattaatctg aggcccaatc tatgtctcac aaatgtaggt tagcctctta cgtgccgaaa
     1921 ggcaaggaga agcaggctat gaagcagcaa aaggcgatgt taatcgccct gatcgtcatc
     1981 tgtttaaccg tcatagtgac ggcactggta acgaggaaag acctctgcga ggtacgaatc
     2041 cgaaccggcc agacggaggt cgctgtcttc acagcttacg aacctgagga gtaagagacc
     2101 cggcggggga gaaatccctc gccaccgctg atgtgtcagg catcctcaac gcacccgcac
     2161 ttaacccgct tcggcgggtt ttgttttttc tggtcgttct ggtttacaat ccatccgtca
     2221 gcctgaacaa ctggcacctg ctgcgccagc agagaaaaca gatggcgcac gataccaaat
     2281 tttacaattc ggataactct gccgcccctg ccagcaggca cgggcggcgt tcccgcactt
     2341 tcaaatctga ctggttccag caccccccat gcactgaaga acaggccgag tggctaattc
     2401 agtgctaccg cagacacgga tacgagatta agaaagccct cagcctcgat tatcgtcact
     2461 ggataatctc cgtcaggctt ccttactccg aacgaccacc gcgtccgtcc cgcacattcc
     2521 agcaacgcat ctggaggtaa cgtgcgggta ttacttcgac ctgttctggt accggaactc
     2581 gggctggtga tcgttaagcc gggccgtgaa tccatgccgg tattccacaa tacccgggta
     2641 ctggtggagc cggaaccgaa aagcatgcgt aatctgccgt ccggggtcgt tcctgccgtt
     2701 cgccagccgc tggcggagga taaatcatta ctgccatttt tcagcgacga acgagtgatt
     2761 cgtgctgctg gtggcgctgg cgcattgtct gactggttac tgcgccatgt taaatcctgc
     2821 cagtggccac acggtgatta tcaccacagt gaaaccgtca ttcaccgtta tggtaccggc
     2881 gcaatggtgt tgtgctggca ctgcgacaac cagctgcgcg accagacctc cgaatcactg
     2941 gaacatctgg ctcaacaaaa cctggcagcc tggatgattg acgtcatacg ccatgcaatg
     3001 aatggctcgc aggagcgtga attatctctg gctgaattat cctggtgggc ggtccgcaat
     3061 caggtggcgg acgcgctacc ggaagcggta ttacgtcgtt cgctggggtt gcgtgcggaa
     3121 aaaatccgct caat
//
LOCUS       DAZGLJ010000336         3033 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000336 DAZGLJ010000000
VERSION     DAZGLJ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.340"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..502
                     /locus_tag="ACHK49_004795"
     CDS             <1..502
                     /locus_tag="ACHK49_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFO9265268.1"
                     /translation="TAQRCKKNPIYSPKIVEQSQITAFDKSTNVRRATAFAISVINDK
                     ATIPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDKNEEVRIEAIIGL
                     SYRKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSAI
                     DKLKRS"
     gene            530..1488
                     /locus_tag="ACHK49_004796"
                     /pseudo
     CDS             530..1488
                     /locus_tag="ACHK49_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1500..1961
                     /gene="yibG"
                     /locus_tag="ACHK49_004797"
     CDS             1500..1961
                     /gene="yibG"
                     /locus_tag="ACHK49_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO9265269.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSA
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            2596..>3033
                     /locus_tag="ACHK49_004798"
     CDS             2596..>3033
                     /locus_tag="ACHK49_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO9265270.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAADCIETVNKIKDKNKKIIDINSVNICSDD"
BASE COUNT          988 a          532 c          667 g          846 t
ORIGIN      
        1 aacggctcag cgatgcaaga aaaacccaat ttattcacct aaaatagtag aacaatctca
       61 aattactgct tttgataaat cgactaatgt cagacgtgct acagcatttg ctatttctgt
      121 tatcaatgat aaagcaacaa ttccactatt gattaatctg ttaaaagatc caaatggaga
      181 tgtcagaaac tgggccgcat ttgcaataaa tatcaataaa tatgataata gtgatattag
      241 ggattgtttt gtggagatgc ttcaggataa aaatgaagaa gtccgtattg aagcaataat
      301 cggactttcc tacagaaaag ataaaagggt tttatctgtt ttatgcgatg agttaaaaaa
      361 aaatactgtt tatgatgata tcattgaagc tgcgggtgaa ttaggtgata aaacgctact
      421 tcctgtttta gatactatgt tgtacaagtt tgatgacaat gaaattataa cttccgctat
      481 tgataagctg aagcgttcat agcgtgagtt gcctatgcac agtgggggat tcccgccggc
      541 acggtgcaga tgctcgaccg gctggaaagt gaaatcctgg ctgaccgggt gagtgaggaa
      601 agccgccgct ggctggcatc gtgcggcctg accgtggagc agatgaaaaa ccagatggac
      661 ccggtgtaca cgccggcgcg aaaaatccac ctgtaccact gcgaccatcg cggcctgccg
      721 ctggcgctca tcagcacgga aggggcaaca gcgtggtgcg cagaatatga tgaatggggc
      781 aacctgctga gtgatgagaa cccgcatcat ctgcagcagc tcattcgcct gccgggtcag
      841 cagtatgatg aggagtccgg cctgtattac aaccgccacc gctattatga cccgctgcag
      901 gggcggtata tcactcagga tccgattggg ctgaaggggg gatggaattt ttatcagtat
      961 ccgttgaatc cggtcataaa tgtagatccg caaggtttgg ttgatataaa tttatacccc
     1021 gaaagtgatc ttatccattc tgtagctgat gagattaata tcccaggcgt tttcacaatc
     1081 ggggggcatg gtacccccac atctattgaa tccgcaacgc gcagtatcat gacagctaaa
     1141 gatctagcat atctaattaa atttgatggg aattataaag atgggatgac agtttggtta
     1201 ttttcttgta atacaggtaa aggacaaaat tcatttgcta gccaattagc taaagagtta
     1261 catacaaatg taataggacc tgacacgcta tggacgtggt gggggcgagg aactaatggt
     1321 aagttaaaaa tggatacagt gctaacagca ccaacgaacc ttaattcaaa taaggatcta
     1381 atggctataa caacaaaaga ccttggtaat tggataacat atgggccatc tgggcacccc
     1441 atttctaata tgcaaggtac gccagaaaaa cccagtgata taagataggt tgtagatgta
     1501 tgaaagcatg cttgttacta tttttttatt tttctcttat tggtcaattg catggtgctg
     1561 atgtgaaaat aaaagaaaac gaaagtgtga tgggatctac agcaatgacc tatgatttaa
     1621 gtgaagaaaa gctgatgaaa ctgaagtata aatcacaaca tggtgattca gaggcatcat
     1681 ttcgactata tcaatattac tgctttacta aaaataatat tgataaacaa ttgcgattct
     1741 tggaaagatc agcatctcag gggaatgtta cagcgcagtt taattatggg gtctttttat
     1801 cagctacaaa tccaacatta tcagaatatt ataatttgaa tagagctatt tattggatgg
     1861 aattcgctgt aaataacggt aatattgatg caaagagcaa acttcaagag ctcaaaaagc
     1921 taaagcgaat ggatagaagg aagaataagg agaatccgta aaagcaatag ttcatcagac
     1981 tgccgcagca gtagtatgat gatgaatcag ggctgtacta taactattat cggtaacatt
     2041 aaccgcagca ggggcggtat aacactcagg ctccgattgg gctggaaggt aggtggaatt
     2101 tttatcagta tccgttgaac ccgatttctg gaatcgatcc ccttgggatt agccacctgt
     2161 ttatacagta taactttagg tatgctttcg tgtgtttcag atataccatg tgatgataat
     2221 ttttatgatg tgcttagtat tccagtcgct tctggtaata atatgcaatg taaaaataat
     2281 ccccgttgta cacatttata aatagatggc caatacctca aggcctatgg agttagaatg
     2341 ttaatgaccc gggagcaagc aatagaaaac ctgacggcat acgattagta ccgtcagtta
     2401 atacaggaac ttataatcgc aatggatttt caatacatag ttgtttaaac gcttttggtc
     2461 cgtctttagg acctcgattc tgttccgagg gttgtataac tggtttatct aatgacatgc
     2521 aaaaattaaa tgagttaata ttttcagagc cagatagcac attaacagtt acggactaaa
     2581 aaatatggca aggttattaa caattgtatt tttttctcta tttctgcgag tgcagttact
     2641 gcaagacaat ttcagagaga gttagatttg gcagacaaag caatattatg ggcactaata
     2701 tcagccagta ctaaagaggg acgaaaagcg tgttcactaa gctattttgc ctgtaaggcc
     2761 gcagaggctg aactcggact ggcatatatg gctgcgaatg ataacaagga atttctcaca
     2821 tccttatcta atataatgag gtacaaaata gatgctggac tttccgaatc ctatacatgc
     2881 tatttattaa gtaagggaaa gatcataaga ccatatctga aaaatctaaa tccactccaa
     2941 cttgccgctg attgcattga aacagtaaat aaaataaaag ataagaataa aaaaatcatt
     3001 gatataaatt cagttaatat ttgtagtgat gat
//
LOCUS       DAZGLJ010000337         3029 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000337 DAZGLJ010000000
VERSION     DAZGLJ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.341"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..467
                     /locus_tag="ACHK49_004799"
                     /pseudo
     CDS             1..467
                     /locus_tag="ACHK49_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178393.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            458..2107
                     /gene="espL2"
                     /locus_tag="ACHK49_004800"
     CDS             458..2107
                     /gene="espL2"
                     /locus_tag="ACHK49_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL2"
                     /protein_id="HFO9265271.1"
                     /translation="MPIINKSASNYVEYISKNNPPYLSKKRDASINLNGKVSDCNGEI
                     IWCRHIASYWSEFFCSNSGKIDYETFSSPQLLSKAIVIQENKGTNNIKGDVYFVENES
                     WGSVIYNLFLQLEKENKSHTSLEVHSPGHAMALGIKIKNDKENKFVINFYDPNQTATH
                     KRVFFCTNNICDIINLTAYDFLSEQCLKCYGLKEDTLSLFVDKTKSNDNNNVFKKKLP
                     DNILQGVVINFAMGAGLREIIKKVYNDTRFTDLTKSQMKILCESKNVNNVPGLLLALQ
                     NGHDNVIDEYGILIKKSNLNKEELIHILSARTLDGTIPGLYQALQNGHAQAIKSYGNL
                     VLDTIDKNIDLEYLLSAFKYEAHSSNKYTPGLFSAFQNGHADAIKAYCGVLGNSNLKR
                     GEIIRMLEARNYDGAPGLLLAYQNGDINTIQSFFDSLIMLDISKDFIEELLTAKHYDF
                     TGLSLAISHRHDHVVKLYGKLFKKLDTSPYKMSIILALAIDCERNNANIIIDSEYKSN
                     KAVKEYVEILKEFNICPEKVAEYLSEFSGKHFLDVYNYYSN"
     gene            2289..2474
                     /locus_tag="ACHK49_004801"
     CDS             2289..2474
                     /locus_tag="ACHK49_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO9265272.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRTIYFSRTV
                     VRNKKVIGTFIKKHIFY"
     gene            2715..>3029
                     /gene="nleB"
                     /locus_tag="ACHK49_004802"
     CDS             2715..>3029
                     /gene="nleB"
                     /locus_tag="ACHK49_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFO9265273.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYPLEPIDEKTP
                     ILFQWFEARPERYEKGEVPILNTKEHPYLSNIINAAKIENERIIGVLVDGNFTYEQKK
                     EFL"
BASE COUNT         1082 a          473 c          549 g          925 t
ORIGIN      
        1 gctgtaccgt tatttcatta ccatgctcga cgaacacagc aactactctc tggccgtact
       61 atcgcttaaa agtgatatca tcaggcattt cttctgctgt gtagcccagt tgttccttgt
      121 tggtatcaac cagatcatca ctgatgatgg gcaaagagtt ctcgggaaaa ttcgacaaaa
      181 cgctacagga agccaccgtc gggttcgtat ggacttatct ctatacgccg aaaataaatg
      241 ccataagtga gcattttaac cgaacactga gggaacaatt tattgagttt aatgaagaat
      301 tgctctttga atagctgaat atgttcaatc tgaaactggc tgagtatctg gtactgtaca
      361 acagtaaaat tcttcataaa tcgtttaaac tacaaaaatc agtagaatat attatttcca
      421 ctgtcaggcg gaaaatttat agaaaggata caaacatatg ccaataataa acaaatcggc
      481 atcaaattat gttgaatata tttcaaaaaa caatccccct tatttatcaa aaaagagaga
      541 tgccagtata aatctaaatg gaaaggttag tgattgcaat ggggaaatca tatggtgtcg
      601 acatatagca tcatattgga gtgagttttt ttgtagtaat tcaggaaaga tagactacga
      661 aacattttca tcccctcaat tattaagcaa agctattgta attcaggaaa acaaggggac
      721 aaataatatt aagggtgatg tgtattttgt tgaaaatgaa tcttggggaa gcgttatata
      781 taaccttttt cttcaacttg agaaagagaa taaatctcac acatcattag aagttcattc
      841 tcccggacat gcaatggctc tgggcattaa aataaaaaac gacaaggaaa ataagtttgt
      901 tattaatttc tatgatccca atcagaccgc gacgcataaa cgagtatttt tttgcactaa
      961 taacatttgt gatataataa acctgacagc atatgatttt cttagtgagc aatgtctgaa
     1021 atgttatggg ctaaaagaag acacactgtc actttttgtg gataaaacaa aatcgaatga
     1081 caacaataat gtgtttaaaa aaaaattacc tgataatatt ctccaaggtg ttgttattaa
     1141 ttttgctatg ggagcaggac taagagagat aataaaaaag gtatataacg atactcgatt
     1201 tacagatctt acaaaatctc agatgaaaat cttgtgtgaa tcaaaaaatg ttaataatgt
     1261 tcctgggttg cttttagctc tacaaaatgg acatgataat gtaattgatg aatatggtat
     1321 tttaattaaa aagtcaaatc ttaataaaga agaattaata catatattat ctgcaagaac
     1381 tttggatgga accatacctg gtttatatca ggcccttcaa aatggacatg ctcaagctat
     1441 taagagttat ggtaacttag ttctggatac tattgataaa aatatagatt tagagtattt
     1501 gctttcagca tttaaatatg aggcacatag ctctaataaa tatactcctg gattattttc
     1561 tgcatttcaa aatggtcatg cagacgcaat aaaggcatat tgtggcgtac ttggcaatag
     1621 taatttgaaa agaggtgaga taatcaggat gcttgaggcg agaaattatg atggtgcacc
     1681 aggattactt cttgcgtatc aaaatggaga tattaatacg attcagtctt tttttgactc
     1741 actaataatg ttagatatat caaaggattt tatcgaagag ttattgactg caaaacatta
     1801 tgattttacg gggttatcat tagcgatcag tcatagacat gatcatgttg tcaaattata
     1861 cggaaagtta tttaaaaaac ttgataccag tccatataaa atgtcaatta tcctagcatt
     1921 agcaattgat tgcgagagaa ataatgcaaa cataattatt gatagtgagt acaagtcaaa
     1981 taaggcagta aaggaatatg tagaaatttt aaaagaattc aatatatgcc cagaaaaggt
     2041 agcagaatac ctaagcgagt tcagcggaaa acatttcctc gatgtatata attattattc
     2101 caattgaaaa aaatataaag tatctatcac ggcactctca gttgagtatc acaggcacat
     2161 gtattttagc cccctgagtc tacagacaac caatattact taaataagtg ataatcttaa
     2221 tgctgttttt tagaacagat gattgatatt tttgggccgg agaaacgcag acaggctttg
     2281 ttgaataaat cgagcttttg ctgagtttaa ggatcagatc actcattttc ctgcaaaagc
     2341 tcattgggcg aaacaatctg actcagagaa cccggattaa acgtctggcc cgcagaacca
     2401 tctatttctc acgcactgta gtacgtaata aaaaagtcat cggtactttt atcaaaaaac
     2461 atatattcta ttaattattg ccatcacctt tttcttattg acaacattac tctgctgatt
     2521 gttaaaatct aacctgtatt cagggttacg attctataca atagaacagg gaggggaaag
     2581 gatgtcatcg ttaatgagac tttttgttaa tgtcaacaat gctatgaatt tattttagaa
     2641 aaacaattaa gctgacgtag tgttccgtct aaatgacggg gcgatattaa catgattaga
     2701 acaagaggaa ttttatgtta tcttcattaa atgtccttca atccagcttc agaggaaaga
     2761 cagccttatc aaatagtaca cttctccaga aagtttcttt tgctggaaaa gaatatcctc
     2821 tggaacctat tgatgaaaaa acccctattc tttttcagtg gtttgaagca aggccagagc
     2881 gatacgaaaa aggagaagta ccaatattga ataccaaaga acatccgtat ttgagcaata
     2941 ttataaatgc tgcaaaaata gaaaatgagc gtataatcgg tgtgctggta gatggaaatt
     3001 ttacttatga acaaaaaaag gaatttctc
//
LOCUS       DAZGLJ010000338         3014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000338 DAZGLJ010000000
VERSION     DAZGLJ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.342"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1074
                     /locus_tag="ACHK49_004803"
     CDS             <1..1074
                     /locus_tag="ACHK49_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO9265274.1"
                     /translation="MRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDY
                     VVKGIIPAVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGI
                     GVPRRVKAWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIIL
                     DTLARCFGGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRA
                     SLDAEYRIRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVV
                     DDPRPPVELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNF
                     RRWLYKLEKDGVIRIDGDDVAPL"
     gene            1275..1526
                     /locus_tag="ACHK49_004804"
     CDS             1275..1526
                     /locus_tag="ACHK49_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265275.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            1523..1828
                     /locus_tag="ACHK49_004805"
     CDS             1523..1828
                     /locus_tag="ACHK49_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265276.1"
                     /translation="MSEPKFGEKLYKHNGLITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            1838..2248
                     /locus_tag="ACHK49_004806"
     CDS             1838..2248
                     /locus_tag="ACHK49_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO9265277.1"
                     /translation="MTAQIAAYGRLVDDPLVKQSSKGTPMTLARMAVSLPCSQAHDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGYQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGDDDPYGDDIPF"
     gene            2259..2531
                     /locus_tag="ACHK49_004807"
     CDS             2259..2531
                     /locus_tag="ACHK49_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO9265278.1"
                     /translation="MVHDRIAEELEAKGFYRRASARWGEVMLLVETDKERHQVTMRRL
                     ECSRKAQRPPEPPDNYGDLKKAVDRTYAEMGMDGAGDEIWRNYQDS"
     gene            2488..2616
                     /locus_tag="ACHK49_004808"
     CDS             2488..2616
                     /locus_tag="ACHK49_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO9265279.1"
                     /translation="MVLVMKYGEITRTANHTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            2657..2881
                     /locus_tag="ACHK49_004809"
     CDS             2657..2881
                     /locus_tag="ACHK49_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265280.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKGIRD"
BASE COUNT          850 a          662 c          893 g          609 t
ORIGIN      
        1 atgcgggaga actggcgcga atacctggaa cgggtgcgtg ggaaagagcc ggcggaagaa
       61 aaaaaccacc agcgaaaaac cacgctaccg atgagcgttg gatctaccgg atacgacacg
      121 caactggatt acgtggttaa ggggattatt ccggcggtat cgctatgcag catatacggg
      181 gctagcgggt cctataaatc attccttgcc ggatcgtggg cgtgccatgt tgccactggt
      241 cgccagtggg gaggccgcag ggttgcgcat ggtgcggttc tctatgtggt tggtgaaggc
      301 ggtataggtg ttccgcgtcg tgtaaaagcc tgggaggttg tgcacgatga gcaggtgaaa
      361 aatctgtatc tggtaaaccg ccccatcttt ccggctgccc cgcttgatgt tgatgaaatg
      421 gttatcgctg cccgtcaggt ggagcgggaa acgggtaaac ctgtacgcat gattattctg
      481 gatacgctgg cgcgttgctt tggtgggaat gatgaaaatg attcccgtga tatgggggcg
      541 tttatccgtg gttgtgacga actgaaacga cgcacagggg ccacggtgct ggtggttcac
      601 cattccggca aggatgagac gaaaggcgcg cgcggttcca gtgcatttcg tgcttcgctg
      661 gatgctgaat accggatacg cagggaggac gcaggaagcg aagcgctggt tatctcatgc
      721 accaaaatga aggacgcgga ggaactcaaa gaagccgcat atgacttacg cgtggtggag
      781 ctttttaccg acgctgacgg tgaattaatc acgtcgctgg tggtggtgga tgatccgcgc
      841 cctcctgttg aactggagcg catcgaggag gcagggaaca agacggaaaa ccataccgcg
      901 ctatgggggt gcatccgttc acgcacacag aacggcgaca agtgcacgat cccgctgtta
      961 cgtgatgaca tgaaaaagct ggggtatgaa atgaaaaact tccggcgctg gctgtacaag
     1021 ctggaaaaag atggggttat tcgtatcgat ggggatgatg tagcgccgct ataaaagtga
     1081 ggagcaaaag cgaggtggat agaaagaggg ccaaaattag cctgctctcc ctcacttttc
     1141 gacctgtata catcctcaaa agtgaggggt aaaaaaatac ttatgaaaca cacacataga
     1201 aaaaccgaaa atcccagctg cgacgaagtg agacgcttga aaaagtgagg cgaaaaagtg
     1261 agaggttgcg agaaatgacc caaaaacgca gagacagaac agagccaaaa tataaagcgt
     1321 tagacatgac tgagcacacc ttaaaggtgg caatcagaac gatagaccgc cacacgcggg
     1381 aaggatacgc gaaggaacat cccgacctga taagcgcatt catgaccacg gcggcggcaa
     1441 actttgccac gctgacagaa cgggagattg ccgaagcgga acaggtaaca accatcaacg
     1501 ttaaaaccgg agaggtggaa tcatgagcga accaaaattt ggtgaaaaac tttacaagca
     1561 caacggactg atcacgattc tgcaaataag cgcagctaca ccaggctggt gggttgaaac
     1621 agacgaagga tcctcacccg tggcatcatg ggcgctttgt gtgcttagtt accctgaccg
     1681 cgatgtgtat caggatattc tgcctgtgat atccactgac aaaggcatga aacccgtaga
     1741 catcaaaaac atgggttttc agtgcgtaat gctcactgaa aaaatgatgg aagagatgaa
     1801 aaagaacaac gccggatcgc ttcactgagg tggcaatatg acagcacaaa ttgcagctta
     1861 cgggcgcctg gtggacgacc cgctggtaaa acagagcagc aagggcacac cgatgactct
     1921 ggcgcgtatg gcggtatcgt tgccatgcag ccaggcacat gacggacagg cgacgttatg
     1981 gttatcggtt atcgcatttg gcaaacaggc cgatttcctg gctaaacatc aaaaaggcga
     2041 tgttgccagc gtatccggca cgatgcaggt aagccagtgg accggacaga acggggaaac
     2101 acggcagggg tatcaggtta ttgcagacag cgtaatcagt gcccgtgcgg cacgtcctgg
     2161 cgggaacaga cgcaaaacca caggcacaca gggtaatcag ccaccagcgg gagacgatga
     2221 cccctacggt gatgatattc cgttctgagg gggtgacgat ggtacatgac cgcatagcgg
     2281 aggaactcga ggcaaaaggc ttttaccgga gggcgtcggc gcgatggggt gaagtcatgc
     2341 tgctggtgga gacagacaag gaacggcatc aggttacgat gcgacggctg gaatgttcca
     2401 ggaaggcaca gaggccaccg gagccgccgg ataattacgg agacctgaaa aaggcggtcg
     2461 atcgcactta tgccgaaatg ggtatggatg gtgctggtga tgaaatatgg cgaaattacc
     2521 aggacagcta atcacacagc cggagaaatc cggctttttt tgcaccagtt gaaacggtat
     2581 ggcgcattac cgggttttcg tcacggtcag gcatagttac tatctgaaac aaacagacac
     2641 aacagaggaa aaaacaatgc cgatgaaatt tgatgagata ttaaaacagc gtgataaata
     2701 ccatgctgac aacatggaga cgatgagcat caatgattac cgcgcattcc tggagacggg
     2761 cgcactgatt gaaaaggatc agcatggttt tgtgagatgt gctctgtccg gtgaaatgct
     2821 ggcggtaaat cctgaacaga tagatgcatt gatagaattt ctgaaaggga tcagggactg
     2881 agcacgcata cagccggagc aatccggctt tttgtcattt tttgtaaatt atttgttcgc
     2941 ggttgttcca cgttgttcac tgacaggatc ggcatatttt acccgaactg aatcatgatt
     3001 attctcgccc gtgg
//
LOCUS       DAZGLJ010000339         2983 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000339 DAZGLJ010000000
VERSION     DAZGLJ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.343"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..534
                     /locus_tag="ACHK49_004810"
     CDS             <1..534
                     /locus_tag="ACHK49_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO9265281.1"
                     /translation="ELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKT
                     EPCHEWDMAGIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVSALMT
                     ALPDHAASPLMTRLQDELCTESVRGQAREQFTGAVDRIFDTVESVCVASVARTVLRAV
                     RDTVVSVARAVWNWIFF"
     gene            906..>2983
                     /gene="cah"
                     /locus_tag="ACHK49_004811"
     CDS             906..>2983
                     /gene="cah"
                     /locus_tag="ACHK49_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HFO9265282.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLL"
BASE COUNT          711 a          768 c          887 g          617 t
ORIGIN      
        1 gaactggact tggtactgtg gctgattaaa gccgatgacc gtgccctgtc tgtggatgag
       61 tatttctggc gacacatact gcaccgggga catcagcagg tgctgtttgt ggtgacgcag
      121 gccgacaaaa cagagccctg ccatgaatgg gatatggcag gtattcagcc ttctcccgca
      181 caggaacaga acattcgcga aaaaacagat gcggtattcc ggctgttccg gcccgtacat
      241 ccggttgttg ccgtatcggc ccgcaccggc tgggaactgg atacgctggt cagtgcgctc
      301 atgacagcgc ttcccgacca tgccgccagt cccctgatga cccgcctgca ggacgagctg
      361 tgcacggaat ctgtccgggg gcaggcccgt gaacagttta ccggtgcggt ggaccggata
      421 tttgacacgg tggagagcgt ctgtgttgcc tctgttgcac gcacagttct gcgtgccgtc
      481 cgtgacacgg tggtctctgt tgcccgcgcg gtatggaact ggattttctt ctgaacagtc
      541 tgccagggga aatgtctgat tacattgcgt taaccaactg aaaacatgat acaaacacct
      601 catgcccctc ctgtttctct tgcgtgactg ctctactgtt aatagaataa aacgatcgat
      661 aaaacaggta tcgatcgttt atatcgatcg tttatagtgg ttaatggcct gcgaaaaggc
      721 agctgaatct cttcatcatg cagaacggaa ttgcacacaa cagactgata cttctctgtc
      781 tgatgaaggg ggagagcgtt tacgcccggg acgtatcgct gtgcccgata actctgtttt
      841 gtacctgccg gtatccactt ttgtgggtac cggctttttt attcaccctc tgtaaggaaa
      901 agctgatgaa acgacatctg aacaccagct acaggctggt atggaatcac attacgggca
      961 ccctggtggt ggcctccgaa ctggcccgct cacggggaaa acgcgccggt gtggcggttg
     1021 cgctgtctct tgctgctgtc acatcagtcc cggcactggc tgctgacaag gttgtacagg
     1081 cgggagaaac cgtgaacgat ggaacactga caaatcatga caaccagatt gtcttcggta
     1141 cggccaacgg aatgaccatc agtaccgggc tggaactggg gccggacagt gaagaaaaca
     1201 ccggtgggca atggatacag aatggcggga tagccggaaa caccactgtc accacaaatg
     1261 gtcgtcaggt cgtgctggag gggggaacag ccagtgatac ggttattcgt gacggcgggg
     1321 gacagagcct gaacggactg gcggtgaaca ccacactgaa taacagaggc gagcagtggg
     1381 tgcatgaggg cggggttgcc accggtacaa ttatcaaccg cgacggttac cagagcgtta
     1441 aaagtggcgg gctggcaaca ggaaccatca tcaacaccgg cgcagaaggc ggccctgatt
     1501 ctgacaactc gtatacgggt cagaaggtcc agggaacagc agaatccacc accatcaaca
     1561 aaaatggacg gcagattatc ttattttccg ggctagcccg tgacactctc atttacgcag
     1621 gtggtgacca gtcggtacac ggaagggccc tgaataccac actgaatggc ggttaccagt
     1681 atgtgcacag ggacggactt gcgctgaaca cggtaattaa cgaggggggc tggcaggttg
     1741 ttaaggcagg tggcgctgcc ggtaacacca ccataaatca gaacggtgaa ctgagggtac
     1801 atgccggcgg ggaagccact gcagtcaccc agaacacggg cggtgcactg gttaccagta
     1861 ctgctgcaac tgtcatcggc acaaaccgtc tggggaattt cacggtggaa aacggtaagg
     1921 ctgacggtgt tgttctggaa tccggcggtc gtctggatgt actggagagc cattcagcac
     1981 agaataccct agtggatgac ggcggtaccc tggcagtgtc tgccggcggt aaggcgacaa
     2041 gtgtcaccat aacatccggt ggtgccctga ttgcagacag tggtgccact gttgagggga
     2101 ccaatgccag cggtaagttc agtattgatg gcacatccgg tcaggccagc ggcctgctgc
     2161 tggaaaatgg cggcagcttt acggttaatg ccgggggaca ggctggcaac accactgtcg
     2221 gacatcgtgg aacactgacg ctggctgccg ggggaagtct gagtggcaga acacagctca
     2281 gtaaaggcgc cagtatggta ctgaatggtg atgtggtcag taccggcgat attgttaacg
     2341 caggggagat tcgctttgat aatcagacga caccgaatgc cgcgctgagc cgtgctgttg
     2401 caaaaagtaa ctccccggta acgttccata aactgaccac cacgaacctc accggccagg
     2461 gcggcaccat caatatgcgt gttcgccttg atggcagcaa tgcctctgac cagctggtga
     2521 ttaatggtgg tcaggcaacc ggcaaaacct ggcttgcgtt tacaaatgtc ggaaacagca
     2581 acctcggggt ggcaaccacc ggacagggta tccgggttgt ggatgcacag aatggcgcca
     2641 ccacagaaga aggtgcgttt gccctgagtc gcccgcttca ggccggcgcc tttaactaca
     2701 ccctgaaccg tgacagcgat gaagactggt acctgcgcag tgaaaatgct tatcgtgctg
     2761 aagtccccct gtatacatcc atgttgacac aggcaatgga ctatgaccgg attctggcag
     2821 gctcccgcag ccatcagacc ggtgtaaacg gtgaaaataa cagcgtccgt ctcagcattc
     2881 agggcggtca tctcggtcac gataacaacg gcggtattgc ccgtggagcc acgccggaaa
     2941 gcagcggcag ctatggcttc gtccgtctgg agggtgacct gct
//
LOCUS       DAZGLJ010000340         2974 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000340 DAZGLJ010000000
VERSION     DAZGLJ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.344"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>2974
                     /locus_tag="ACHK49_004812"
     CDS             <1..>2974
                     /locus_tag="ACHK49_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease fold toxin"
                     /protein_id="HFO9265283.1"
                     /translation="EADARLDYHLFYEYDAADRIIRWSDNDQTWSRFTYDAQGRCVNV
                     TGAEGYYNATLDYGDGCTTVTDGKGTHRYYYDPDGNILREEAPDGSTTTYEWDEFHHL
                     LARHSPAGRVEKFEYNAALGQLSRYTAADGAEWLYRYDERGLLSNITDPAGQTWTQQC
                     DERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQDERRLGIEYDHHNRPETLTDVMGRE
                     HHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSGLERAETGAEGFRYDRHGNLLAYTD
                     GNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKDTLQLTEVINPQGESYRYILDNCGR
                     VTEERDWGGVVWRYRYDADGLCTARVNGLEETILYSRDAAGRLAEVITPEGKTQYAYD
                     KSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRRAIEYHYPDEHTVIRCILPPEDERD
                     RHPDESLLKTTYRYNAAGELTEVILPGDETLTFSRDEAGREVLRHSNRGFACEQGWNA
                     AGQPVSQRAGFFPEETTWGGLLPSLVREYRYDSAGNVSAVTSREDYGRETRREYRLDR
                     NGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQSAGRHRISEETDQYAGGHRLKQAG
                     NTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSRDQLTGYCSAQGEQWEYRHDASGRR
                     TEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYSVRHLVFNGFELISQQFSRVRQAHP
                     SVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWRPGQTSLWGLALSLPADTGYPDPRG
                     EWDPEADPGLLYAGQWQDAESGLCYNRFRYYEPETGMYLVSDPLGLQGGEQTYRYVPN
                     PLGYIDPLGLAKTSVPAEKISLSDKARDLFRQGKVREALDVHYEDLVRRKLGGISQEI
                     AGREYDVVTDKIIAQVKRTYSSIDNPKNFLSKSTRTQIKKTIELAEEQGKEAQFWFKY
                     GVSPKVREYIESKGGKVIL"
BASE COUNT          715 a          799 c          955 g          505 t
ORIGIN      
        1 gaagcggatg cgcggctgga ctaccacctg ttttatgagt acgacgctgc ggaccggatc
       61 atccgctggt ccgataacga ccagacgtgg agccgtttca cctacgatgc acagggccgg
      121 tgcgtgaatg tcaccggggc ggagggctat tacaacgcca cgctggacta tggtgacggc
      181 tgcaccaccg tgacggacgg caagggcact caccgttatt actatgatcc tgacggcaat
      241 attctgcggg aagaagcgcc ggacggcagt accaccacgt atgaatggga tgaattccat
      301 cacctgctgg cccgccactc ccctgccggg cgggtggaga agtttgaata caacgccgca
      361 ctcggtcagt taagccgtta cacggcggca gacggcgcgg agtggctgta ccgctatgat
      421 gagcgcggcc tgctcagcaa catcaccgac cctgccgggc agacatggac gcagcagtgt
      481 gatgagcgcg gcctgccggt aagcctggtg tcgccacagg gcgaagagac ccggctggca
      541 tacaccgctc aggggctgct gtcggggata ttccgccagg atgaacggcg tctgggcata
      601 gagtacgacc accacaaccg gccggaaaca ctcaccgacg tgatgggccg tgaacaccac
      661 accgaataca gcggtcacga cctgccggtg aagatgcgcg gccccggcgg tcagtcagtg
      721 cggttacagt ggcagcagca ccataaactg agcggccttg agcgggcaga aaccggcgca
      781 gaaggattcc gctatgaccg ccacggcaac ctgctggcgt acacggacgg taacggcgtt
      841 gtctggacaa tggaatacgg cccgttcgat ttgccggtgg cgcgaacgga cggtgaaggc
      901 caccgctggc agtaccgcta cgataaagac acgctgcagc tcacagaagt cattaacccg
      961 cagggcgagt cataccgtta tattctggac aactgtggcc gggtgacgga agagcgtgac
     1021 tggggcggcg tggtctggcg ttaccgctat gacgctgatg gcctgtgtac cgccagggtc
     1081 aacggcctgg aggaaaccat cctctacagc cgggacgccg caggccgcct ggcagaagtc
     1141 atcacgccgg aaggcaaaac gcagtatgcg tatgacaaat ccggcaggct gacgggtatc
     1201 ttcagcccgg acggcacatc acagcgcacc ggctatgacg aacgcgggcg ggtgaatgtc
     1261 accactcagg gccgacgggc cattgaatac cactaccccg atgaacacac cgttatccgc
     1321 tgtatcctgc caccggaaga tgaacgcgac agacaccccg atgaatccct gctgaaaacc
     1381 acgtaccgct acaacgccgc cggagaactg acagaggtca ttctgccggg ggatgagacg
     1441 ctgacgttca gccgtgatga ggcgggacgt gaagtgctcc ggcacagtaa ccggggtttt
     1501 gcctgtgaac agggctggaa tgcagccggc cagcctgtca gtcagcgcgc cggatttttt
     1561 ccggaggaaa ccacatgggg cgggctgctc ccctcactgg tacgggagta ccgttacgac
     1621 agcgcgggca atgtgtcggc tgtcaccagc cgggaagatt acggacggga aacccggcgg
     1681 gagtaccggc tggaccggaa cggccaggtc acggcggtga cagcctcagg caccgggctg
     1741 ggctatggcg aaggcgatga gtcctatggc tatgacagtt gtggctacct gaaggcgcag
     1801 tctgcgggca ggcaccggat aagtgaagag actgaccagt atgccggagg ccaccggctg
     1861 aaacaggccg gaaacacgca gtatgactat gacgccgcag gccggatggt cagccgcacc
     1921 agacaccgtg acggctaccg cccggaaaca gagcggttcc ggtgggacag ccgggaccag
     1981 ctgaccgggt attgcagcgc acagggtgag cagtgggaat accgccacga cgccagcggc
     2041 agacgaacag agaaacgctg cgaccggaag aaaatccgct tcacgtacct gtgggacggc
     2101 gacagtattg cggaaatccg ggaataccgc gatgataaac tgtacagcgt acggcacctg
     2161 gtgtttaacg gctttgagct gataagccag cagttcagcc gggtacggca ggcgcatccg
     2221 tcggtggccc cgcagtgggt gacgcggacg aatcatgcgg tgagcgacct gacgggccgc
     2281 ccgctgatgc tctttaacag tgaaggtaaa accgtctggc ggccgggaca gaccagcctg
     2341 tgggggctgg cactcagcct gcccgcagac accggctacc cggacccgcg cggggagtgg
     2401 gacccggaag ccgaccccgg cctgctgtat gcgggacagt ggcaggatgc ggaatcgggg
     2461 ctgtgctata accggttccg gtactacgag ccggaaaccg gaatgtacct ggtgagtgat
     2521 ccgctggggt tgcagggggg tgagcagact taccggtatg tgcctaatcc actggggtat
     2581 attgatccgc tggggctggc aaaaacttct gttcccgcag aaaaaataag cctttcggat
     2641 aaagcaagag atctttttag acaaggaaaa gtaagagaag cattagatgt tcattatgag
     2701 gatcttgtac gaagaaagct tggtggtata tctcaagaaa ttgccgggcg tgaatatgat
     2761 gttgttactg acaagatcat agcgcaggtc aagaggactt atagctctat agataatcca
     2821 aaaaacttct taagtaagtc tactcggaca caaattaaga agacaattga gctggctgaa
     2881 gaacagggga aagaggctca gttttggttt aaatatggag taagccctaa agtaagagaa
     2941 tatatagaaa gcaaaggtgg gaaagttata ttgg
//
LOCUS       DAZGLJ010000341         2917 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000341 DAZGLJ010000000
VERSION     DAZGLJ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.345"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(330..1007)
                     /locus_tag="ACHK49_004813"
     CDS             complement(330..1007)
                     /locus_tag="ACHK49_004813"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO9265284.1"
                     /translation="MWHYLHYKVSRGKPHAECDAKRRAGSPPGPDAHQLQRSQHKKSR
                     QQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADARRIVSHYEQRWLIEEYHKAWK
                     SGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGSISEETQNDSCEKILTPTEWKLLWV
                     KLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPGWVVMWDGWFRHQDMAEGYLVM
                     KSLDQEI"
     gene            1205..2824
                     /locus_tag="ACHK49_004814"
     CDS             1205..2824
                     /locus_tag="ACHK49_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEc38 family transposase"
                     /protein_id="HFO9265285.1"
                     /translation="MGNSMHSLKTLLQLPCGWRCSRQIISSDGITLHLHGKRKKAQCP
                     ECFKRSDSVHSCRRRRIQHLPCSGHTLWLVFSVRHWYCRNPACSRKIFAGSLAPFAGS
                     HQQSSQALQNLQRQLGLIAGGEAGKRAATAVGLRCSADTLLRRVINTPETKQSGAPHV
                     GIDEWAWHRGHRYGTLIVNLDTHRPLVLLPGRDQRTLATWFRKYPEIQIVSRDRSGVY
                     ATAAREGAPQARQVADRWHLLKNIGDALERMMYRHIPLIRLVASELSPKKSPDPEPSV
                     PAASLRRPERLKQQTRKKRHQRWTEVMALHNKECSFREISRITGLSRVTVSRWVRSGT
                     FPEMSTRPPKRGLLDPWREWLKEQRESSNYNASRIWREMVARGFTGSETIVRDAVAKW
                     RKGWNPPVTTAVRLPSVSRVSRWLMPWRITRDEENYASRFISLMCEKEPELKIAQQLA
                     LEFYRILKTQNKSQLSSWFNRVHESGSAEFRRVAAGMEADAAAICEAISSRWSNGVVE
                     GHVNRLKMLKRQMYGRAGFELLRQRVMSPLA"
BASE COUNT          694 a          766 c          723 g          734 t
ORIGIN      
        1 cgccattctg agctcaatag tatccatgcc actgagctta caggtcttta tcgggcgata
       61 gtgctttatt cttcggggct ttgtggatga tttttcgaaa tgttcatgat agttaggtaa
      121 agctaccgca tatcatttgc aaaatgacag atagatatac ttcaatttga atcttaacaa
      181 ttagtatggg aatgctgtca atcctctgta tgcgtccacc acatattgaa atttttgttc
      241 tctaatatat tccacgggcg tcaagagtgt gaggcctgtg ctgttataca gcaccagacg
      301 ttgtccccca tctttgacct gtctcttgat cagatctcct gatcaagaga cttcatcacc
      361 aggtaaccct cagccatatc ctgatgtctg aaccagccat cccacataac tacccaaccg
      421 gggctgcttg tgcgtttgct gtcatgccat cgccccagtt tagccagttt caaacaggcc
      481 catttcaggg tcggtgtttg cgctggaagc tgctttcctt ccagcttaac ccacagtagt
      541 ttccactctg tcggcgtcag tattttctca cagctgtcat tttgtgtttc ttcactgata
      601 cttccctgcc gcaggcccag cacccgcacc gcgataacca ccatgcgctc aaggttatcc
      661 cgggtctgca ttcgcagcga ttccacacat gtaccaccac ttttccacgc tttgtggtat
      721 tcctctatca gccagcgttg ctcgtaatgg ctgacgatac gtcgcgcatc ggcggcactc
      781 gccacttttt ctgatgtcag cggataccag caggctccgt cctctcctgc tccccgcaac
      841 agacatacgt gagcgagagc gcctggccgc tgttgtcggg attttttatg ctgacttcgc
      901 tgtaactgat gagcatccgg gcctggcggg ctgcccgccc gcctttttgc atcacattca
      961 gcgtgtggct tcccgcggct gactttataa tgcaggtaat gccagatatc cgcttcccgg
     1021 tcacagacag tgattacctg tttctgtatc tcccccagcc gttcggccat gcgctccgaa
     1081 gcctgctgcc agcggtaact ttctttttct gtcacgaacg gtgcaatagt ggttcttccg
     1141 caacttgggt ggaagaaaat ggatgaaact ttctggtgaa caaagatcta agctgtttta
     1201 agtcatgggc aactcaatgc actctcttaa gacacttcta cagttacctt gcggatggcg
     1261 atgcagtcga caaattatta gctctgacgg tataaccctc catctccacg gaaaacgcaa
     1321 aaaagcacaa tgtcctgaat gctttaagcg tagcgactct gttcatagtt gtcgtcggcg
     1381 ccggatacag catctaccct gctcaggaca tacgctatgg cttgtatttt ccgtccgcca
     1441 ctggtactgc cgtaacccgg cttgttcacg taaaattttt gccgggtcgc ttgctccctt
     1501 cgccggttca caccaacagt cttcacaggc gttacaaaat ttacaacgtc aactgggatt
     1561 aatcgccgga ggtgaggccg ggaaacgggc agcaacggca gtggggctcc gttgcagtgc
     1621 tgatactctc cttcgcaggg ttatcaatac cccggagacg aaacagtcag gcgcgcctca
     1681 tgtcggtatt gatgagtggg cgtggcatcg gggccaccgt tacggtacgt taatcgtcaa
     1741 tcttgatact caccgtcccc tcgtcctgct tcccggccgt gatcagcgta cgctggcgac
     1801 ctggttcaga aaatatccgg aaatacaaat tgtctcgcgt gatcgcagtg gagtctatgc
     1861 gacagcagca cgtgaaggtg cacctcaggc cagacaggtg gccgatcgat ggcatctgct
     1921 aaaaaatatt ggcgatgcgc ttgaacgaat gatgtacaga catatacctc tgatacgtct
     1981 tgttgccagt gagttgtcac caaagaaatc acctgatcca gaaccatctg tgcctgcagc
     2041 atcgctccgt cgtccggaac gcctaaaaca acaaacccgc aaaaaacggc atcagcgttg
     2101 gacagaggtt atggccctgc ataacaagga atgtagtttc agggaaatat cccgtattac
     2161 aggcctgtcg cgtgtgacag tcagtcgctg ggtgcgttca ggaacattcc ctgaaatgtc
     2221 aacccgacct ccaaagcgag ggcttctgga cccatggagg gagtggttaa aagagcaacg
     2281 agaaagtagt aattataacg ccagccggat atggcgggaa atggtggccc ggggatttac
     2341 aggcagtgaa accatcgtca gggatgcggt tgccaaatgg cgtaaaggct ggaacccacc
     2401 ggttactact gccgtcagac ttccttcagt gtcccgggta agccgctggt tgatgccctg
     2461 gagaataacc agggatgaag aaaattatgc ttcccgattt attagtctga tgtgtgaaaa
     2521 agaacctgag ctgaaaatag cgcagcaact ggcactcgag ttctaccgta ttctgaaaac
     2581 ccaaaataaa tcacagctaa gcagttggtt caatcgagtc cacgaaagcg gttcagcaga
     2641 atttcggcgc gtggcggcgg ggatggaagc tgatgctgca gctatatgtg aggcaatcag
     2701 tagtcgctgg agtaatggtg ttgtcgaagg tcatgtaaat cgcctgaaga tgctgaaacg
     2761 ccagatgtat ggtcgagcag gatttgaact actcaggcag agggtaatga gtccactggc
     2821 atgaaaatct ccacccaggt tgcggaagag ccactttccg tcgttgacac tgacgacatc
     2881 ttcctgctga aacagcgcct ggccgaacag gaagcgc
//
LOCUS       DAZGLJ010000342         2886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000342 DAZGLJ010000000
VERSION     DAZGLJ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.346"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            176..1081
                     /locus_tag="ACHK49_004815"
     CDS             176..1081
                     /locus_tag="ACHK49_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO9265286.1"
                     /translation="MSRSQNLRHNVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARKPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQHHR"
     gene            complement(1252..>2886)
                     /locus_tag="ACHK49_004816"
     CDS             complement(1252..>2886)
                     /locus_tag="ACHK49_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO9265287.1"
                     /translation="ILILTLVVSLTSVLTRLSPVQIPLPLIQIAAGAVLAQPIFGLHV
                     EFNPELFLLLFIPPLLFAESSKIQPKELIKHSREIISLALVLVLITIFGVGYVIHLLL
                     PNVPLIAAFALAAVLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGLVALKFA
                     VAVTMGTMEFSVHGATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEPSIQIVL
                     MLLLPFIVYLVAEHFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWSMLEYVF
                     NGMVFLILGLQLPGIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGWLWLMRL
                     YSRKRPGKHPMLFAGYKTRHMLLATFAGVRGAITLAGVLSIPLLLPDGTPFPGRYLLV
                     FLATGVILFSLVCGIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSVQKTEQR
                     LLRTSTENLDNELIMETGARVIGYMRRSEDDMSENDNSLHMQRAKLERTFNLAALNAR
                     RAAIYQMRARQEVSDETLAKIVSELDVLETLIMDSR"
BASE COUNT          835 a          714 c          622 g          715 t
ORIGIN      
        1 ttatttcctg acaaattaat actttcattg tggacatgat tcaccgctcc ttttatttta
       61 tgactttcag atatcgtcga atttataaga gcacctgaat ttacccataa aatatgcgga
      121 tgatcacgca taaaaatgtt ttaaatcggt ttttaatgcc ggactggagg gaagaatgag
      181 tcgttcgcag aatttacgtc ataacgtcat aaatcaggtg attgaagata tggcgagggg
      241 caatattcct tctccgctgc cttcacaaag tgggctggca gaaatgtata acatcagtcg
      301 cactacggta cgccacatac tccagcattt aagcgcatgt ggcgtgctca ccctggtggg
      361 gaaaaattat gttatcgccc gcaaacccga acagaacgat ggatttgatt gcatcacggc
      421 ctcgctgaaa gagcaaaacc gtcttttcga gcaggcattt ttcaccatga ttaacctgcg
      481 ccagttacgc gcgggagaaa gcttttccga acttcagctg gcgcgagcgg caggcgtcag
      541 tccggtagta gtacgagaat atcttttgaa attcggtcgt tataatctta ttcaaagcga
      601 aaagcgcggc cagtggagca tgaagcagtt cgatcaatcc tacgccgagc agctgtttga
      661 gctacgcgaa atgctcgaaa cacattccct gcaacacttt ctcaaccttc ccgatgacga
      721 tccccgctgg ttgcaggcga aaaccatgct cgaacgtcac aggacattgc gcgacagcat
      781 tggcagcaac tttcggatgt tttctcaatt agaccgtgat tttcatgcca tgctgttatc
      841 cgctgctgac aacatctttt ttaatcaatc acttgaaatt atctcggtga tctttcattt
      901 tcactatcaa tgggatgaaa gcgatctcaa gcagcgcaat atcattgctg ttgatgagca
      961 tatgaccatt ctcagcgccc tgatttgccg tagcgattta gatgcaataa cggcattgcg
     1021 aaaccatctg gataccgcca aacaatcaat gattcgttcc atcaggcaac atcaccgata
     1081 aattaagtac agattaaaaa cattgattct tggtttatat cactatgttg tattaatgca
     1141 gtattaatcc aggcgattgt tctgcccata aggaaataca tagggaataa acgatgctgg
     1201 aagtattact ctcagccatc atttaactcc ctattactgg tagcgataaa attatcggct
     1261 atccatgatt aacgtttcca gcacatctaa ttcactgact atttttgcca gtgtttcatc
     1321 actgacttct tgccgtgcac gcatctggta aatagccgca cgcctggcat ttaacgctgc
     1381 cagattaaat gttctttcca gcttcgcgcg ctgcatatgt aaagaattat cattttccga
     1441 catatcatct tcgctacgcc gcatataacc gataactctg gcccctgttt ccataatcaa
     1501 ctcgttgtcg aggttttctg tactggtgcg tagcaaacgt tgctcagtct tttgcacact
     1561 gagtatggcc attttggcca taaacagatc ggcagcttcc tcttcttttt gccggttatc
     1621 gctgaaaatg cccacatttt tccccagtag taacggcaga ataataatgc cgcaaaccag
     1681 cgaaaagagg atcacacctg tggcgagaaa taccagtaag taacgcccgg gaaaaggtgt
     1741 gccatccggc agcaataacg ggatagaaag cacccccgca agcgtgatgg ctccgcgcac
     1801 ccccgcaaag gtcgccagca gcatatgacg cgttttatat ccagcaaaca gcatcggatg
     1861 tttgccgggt cgtttacggc tatacagacg catcagccaa agccagccca ggcgcacaac
     1921 cattaacgcc aggtagatcc aaatgacatc aaggagcagc gatccggtgt ctaccagtgg
     1981 atcgttttct gcacggatca gtgattccga aataatgccc ggcagttgta agcctaaaat
     2041 cagaaacacc atgccgttaa agacatattc aagcatcgac cagctacttt tagctttaat
     2101 tctcaggcgg ataggcgcgt ggtggactac gccaatgcga ctggttgtta accccgcggc
     2161 gacagcggca agaatgccag ataagccaaa atgttccgca accagataaa caataaacgg
     2221 caacagaagc atcaatacaa tctgaatgga aggttcatcg tgagcatatc gactgatcaa
     2281 cagcagccct ttgccatata accaggtaac agcaatcccc acggcaatac cgcccaacgc
     2341 aacgacaaaa aacgcaatgg ttgcgccatg aacgctgaat tccatcgttc ccatcgtcac
     2401 tgcaacagca aatttcaacg ccaccaggcc ggaagcatca ttcatcaacg cttcgccttc
     2461 gagcacttcc tggatgtttt tgctgatacg ccctttcccg acaatcccca gcaacgccac
     2521 ggcatcggta ggcgacaaca cggcggctaa tgcgaaagca gcgatgagcg gcacattcgg
     2581 taagagtaaa tgaatcacat agccgacacc aaaaatagtg attagcacca acacaagcgc
     2641 caggctgata atttcccgac tgtgcttgat caattcttta ggttgtattt ttgagctttc
     2701 agcaaacaac aaggggggta taaacaataa gagaaaaagt tcaggattga attccacatg
     2761 cagaccaaat atgggctggg ccagtacagc gccagcggca atctggatca aaggaagtgg
     2821 tatctgaaca ggagacagac gcgtaagcac gctggttaat gatactacca acgtaagaat
     2881 taaaat
//
LOCUS       DAZGLJ010000343         2884 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000343 DAZGLJ010000000
VERSION     DAZGLJ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.347"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(50..652)
                     /locus_tag="ACHK49_004817"
     CDS             complement(50..652)
                     /locus_tag="ACHK49_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265288.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(739..945)
                     /locus_tag="ACHK49_004818"
     CDS             complement(739..945)
                     /locus_tag="ACHK49_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9265289.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1697..1846
                     /locus_tag="ACHK49_004819"
     CDS             1697..1846
                     /locus_tag="ACHK49_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015704109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO9265290.1"
                     /translation="MEKVISHTRYKLTPAEMEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVI"
     gene            2658..>2884
                     /locus_tag="ACHK49_004820"
     CDS             2658..>2884
                     /locus_tag="ACHK49_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO9265291.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPT"
BASE COUNT          749 a          687 c          765 g          683 t
ORIGIN      
        1 tcagccctgg caccctgccc ggataccagc ttccctgaaa caactggcat caggtgcgct
       61 tatcgtcaac cgttgtgcgg tatgccagga gcacgtactg atccatcaga tccgtcagtg
      121 ctgcgctctg cttctgacgg ttgatgatcc attcctccag ctctttttct gatttccgtt
      181 tcagattctc cgtccagtcg tcaaggcagt ttccggtcat taaatcaagc cagcaaagcc
      241 agaccagttt gaggcgcaac tccgtgccac caaactgacg gaagaggctg atcagtttat
      301 ttgccagcga acgctgcctc agacgttcac attcagacag gagaatgcct tccagcatcg
      361 gatgatgaac cagcagatgt tcactggtgc ataaattcac tatcagatca tcatccaggg
      421 gcggcgtggt ccgtttgtcc ggaccataaa tcagccggtg cagactctgg ggtacgatgg
      481 caatctccgg tttttcacca ttgaatgaca cccacccggg cgtcagatta tggaagtagt
      541 agatattcgg atgacacacg ccccaggata cggcgtgccg ggtgaagccg gtcagccatt
      601 tctggtagct ttcgtggagc agctcgtaac gggtctgata ctgtggtttc attttcatat
      661 tcctcacggg ggataacagg cagcagaacg ccgccggcag gccggagact gcgtctgctg
      721 cagatgaacg gttaagaatt acggacggga ctgtgcaata cgcgcctgca gccaggcttc
      781 cacttcgctt ttcagccatc gggagctgcg gcccattttg atgggcgcag gaaagtcacc
      841 atccttgatg agtttgtaaa accacttatc ggtcaggccg gtcagttgag tgataaatgc
      901 catgtcgacc atctggtcat ccatcagtga aactggggtg atcatgagaa tgttcctccg
      961 gatgttgtta aaccagccgg tgtggtcatg aaaacaacgc accaccttca ccggcataac
     1021 aaccggggag aaacgtaaaa aataatcggg aatgtgaccg ccaggagata aacggggagg
     1081 ttacggtggc tgagccgtac cggtatgccg ggtggttgtt acgaaacgat gcttccgcac
     1141 gcgcgcgcga taaagaaggg atgattttcg tgaggaagag gggaaaactg gcgttctgat
     1201 aattgcagag ttatttacgg ggcggttacg atggggcaga ccgtgtccgg tgggcaggcg
     1261 acaggtatca ccgatgggat aagactgtct gattggtatt ataaatatat aagagagatc
     1321 aaacacatga gatcaaatac atgagttcat atatgtgaaa taaacacggt gagtgtgatc
     1381 agtgggcatg atgagaacgc agtagcaaca gactgcgcta tccctgatag ctggcgtata
     1441 acctgcgtat ataaaacgta ccagcaacgc acgtgaacag agtgcattaa gcatccgtcg
     1501 ttgacaccga cctggtgagt gacagtctga gagaccggtt aagtggtggt acgggagtaa
     1561 tgagtttggg gagggtacca gaatgtcagt ctggtacggt atgctgacag tatcacgtta
     1621 attgcagcat aaagtgatga gaggctaatg gaagttaaaa ccagagcagt tccgtcgttg
     1681 ttcatcccgg gagacactgg aaaaagtgat ttcccacacg cgttataagc ttactccagc
     1741 ggagatggaa gccttcaact ctgcggtcga tcaccgactg gcagaactga caatgaacaa
     1801 gctttacgat cgtgttcccg cttcagtctg gaaatatgtc atctgagcgt tcggtaatca
     1861 caactgcatc gcgctaggtg ttatccgctt tgtgattctt attggctctt ctcaggttat
     1921 taaaagctga atatgttcct gttgtcggga accaggataa taccgggagc gttaagcggt
     1981 atagcgacaa caaagaattc gaaatcaaaa gggaatggtg ccggaggcgt gggcggtaat
     2041 aggctgattt tcaggtatta ccggcttaca acgtgtggtt gatattgagg ggctgtgagt
     2101 ttcgcgtatt tgtcagaaac ccagatgagt tttcctgtat gggacgaaat aaggcagcgg
     2161 caatacctgg aaaatttaat atggtatgaa ggagaacctg tcggttacgc ttaatataca
     2221 cggtacactg tcagctcagg ggatacatga accgttatct caccagctac acagtataca
     2281 cacactgacg ctgacagtag tcagcaggac cacaataatc agcgccagca gtatggtaac
     2341 gatacggtct gtattccggt agtagccgaa ggggtcgaca gaaccacaat acgggcaaca
     2401 cactgtatta ttagtaatcg cgtttccaca gtccctgcac ttcttttgcg tatcaccata
     2461 actccctcct cacagcctta cccgtaaccg gttttttaca ttaaaaaaca acgctgaaaa
     2521 ttcaaccgtc agtccggaga cgaccgttcg ggttatcaca gagtgcctga gacagtgtcc
     2581 tgccggaggt cacttcaggg actctgcgca ttttttacga cgtggctatt ccgtatgaac
     2641 catacggaga tttaaccatg acctacaaat acaacccctt ctggcagcaa cgtattcgtg
     2701 agacggtgcg gcacgcactg gatgttcatc cccgtctgac ggcattgcgg gttgatctgc
     2761 gtctcccgga tgtaccggca gcaacggacg cagctgtgat atcccgcttc atcaatgccc
     2821 tgaaagcccg aatcgacgct taccagaaac gtaagcatcg ggaaggtaaa cgcgtgcatc
     2881 ccac
//
LOCUS       DAZGLJ010000344         2874 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000344 DAZGLJ010000000
VERSION     DAZGLJ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.348"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(89..1279)
                     /gene="aspC"
                     /locus_tag="ACHK49_004821"
     CDS             complement(89..1279)
                     /gene="aspC"
                     /locus_tag="ACHK49_004821"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO9265292.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMALLCEAI
                     VAVL"
     gene            complement(1464..2552)
                     /gene="ompF"
                     /locus_tag="ACHK49_004822"
     CDS             complement(1464..2552)
                     /gene="ompF"
                     /locus_tag="ACHK49_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO9265293.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT          747 a          687 c          671 g          769 t
ORIGIN      
        1 aaggcctgat aagcgtagcg catcaggcaa tgttgcgttt gtcatcagtc tcagcccgct
       61 tttcagcggg cttcattgtt tttaatgctt acagcactgc cacaatcgct tcgcacagca
      121 gagccatgtt atctggtgtc atcccggcca catttacgcg accagaagca accgcatata
      181 cgccaaactc ttcgcgcaga cgcagcactt gttcttttgt caggccactg aaggagaaca
      241 tgccgttctg tttgatgata aagctgaagt cgcggtttgc gcctttttcc tgcagcgtat
      301 tgacgaacaa ctgacgcata cgctgaatac gctggcgcat atcagtcagc tcttgttccc
      361 aaatcgcacg taacgcatcg ttgctcagga tggtggcaac aacagaagcg ccgtgtgctg
      421 gtgggttaga gtagttagcg cgaatcgccg ctttcatttg gctgaatgcg cgatcaacgg
      481 tttcactgtc ggcagcaacc agagtacaag cgccaacacg ctcgttgtac aggccaaagt
      541 ttttagagta ggaactggca acaatcagct ctttatgcat agccgcgaaa gcgcgcagtc
      601 cttcagcatc ttcttccaga ccacgggcaa aaccctggta agcgaagtca aacagcggta
      661 accagccttt ctcaactgag agttgtgcca gtgtctgcca ttgttccagc gtagggtcga
      721 taccggttgg gttatggcag cagccatgga acagcactac gtcgccagcc tgagcttcgt
      781 tcaggctgtt aatcagtgca tcgaagtcaa gggtgtggtt ttctgcatca taataagcgt
      841 attcacgaac ttccagacct gcagagttaa agacgctctt atggttcggc cagcttgggt
      901 tgctcaccca cactcgctta acgctggtat tttttgccag gaaatcggca gctacgcgta
      961 gtgcgccagt gccccccgga gtctgtgccg tgcgagcacg tttgtcattg atcagggcgc
     1021 tacctttacc aaacagcagt tcctgagtgc agcgaccaaa ttcagggatg ccgtcaatgc
     1081 cgaggtaatt tttggtggtt tcattttcga gcagatactg ttcagccttt ttcacgctgg
     1141 tcaataccgg ggttttgccc gtctcatctt tatagacacc aatcccgagg ttaattttgc
     1201 cgggacgttc atcggcacga aacagatcgg ccaggcccag aatcgggtcg gcaggagcgg
     1261 cggtaatgtt ctcaaacatg acgaggttcc attatggtta cagaagggaa gtccgctatc
     1321 agggtaacgg gagatttaca aaattccaac tattactgat gaaaacgcag gctgtttttg
     1381 caagacgtga gattgctctg gaaggcataa aaaaaacagg accaaagtcc tgttttttcg
     1441 gcatttaaca aagaggtgtg ctattagaac tggtaaacga tacccacagc aacggtgtcg
     1501 tcagaaccta cgcccagttt gttgtcagaa tcgatctggt tgatgatgta gtcaacatag
     1561 gtggacatgt ttttgttgaa gtagtaggtt gcgcccactt caaagtagtt caccagatca
     1621 acatcaccga taccttctac gtctttcgct ttagatttgg tgtaagcgat ggacggacgc
     1681 agaccgaaat cgaactggta ttgcgcaact aacagaacgt cttgcgtttt gttggcgaag
     1741 ccgctggtgt ttgtaaattt attagtgatc ggcgtagcgt tacgggtttc accgtagttc
     1801 gctgccaggt agatgttgtt cgcgtcgtac ttcagaccag tagcccactg ttcagctttt
     1861 ttaccgttgc caagaggttg agcttcttgc aggttggtac ggtcagctgc accataagca
     1921 ccaacgatac caaagccttc gtattcgtag ctgatagaac cgccaacacc gtcgccgtta
     1981 gaacggcgtg cagtgtcacg ctcgttttta cccaggtact gaacagcgaa gttcaggcca
     2041 tcaaccagac caaagaagtt ggagttacga taggtagcaa cgccgccaac acgaccaacg
     2101 aagaagtcat cgctgtatgc agtatcacca ccaaattctg gcagcatatc ggtgtaaccc
     2161 agtgcatcat aaaccacacc gtagttacgg ccgtaatcga aagaaccaac gtcagcgtat
     2221 ttaagacccg cgaatgccag acgcgttttg ttaccagttt gagcgtcagc gccttcagag
     2281 ttgttaccct ggaagttata ttcccactga ccataaccgg tcagatcgga attgatttga
     2341 gtttcccctt taaaaccaag acgggcatag gtcatgtcgc cattgccacc gtaactgttt
     2401 tcaccgttac ccttggaaaa ataatgcaga ccaacagctt taccgtacag atctactttg
     2461 ttgccatctt tgttatagat ttctgcagcg tttgcagtac ctgctactaa cagagcaggg
     2521 acgatcactg ccagaatatt gcgcttcatc attatttatt accctcatgg ttttttttat
     2581 gacacctgcc actgccgtca ataagttctg tcaataaaaa tttacggaac tattgatgag
     2641 agtttggtgt ctttatgtgt ctgcaggcat ctttccattc aaactaacgt ttcgctaccg
     2701 tgaaagtgct acaaagataa agatttagtt tcaaaaagaa aaaatatgta accaaaagta
     2761 aaaattaagg aactttgtga acaccgtcat atttccatag aaacgtgatg atatttacag
     2821 caatttttat ctatttatat gatttactta tatttaaatt aactaaacgg aaat
//
LOCUS       DAZGLJ010000345         2830 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000345 DAZGLJ010000000
VERSION     DAZGLJ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.349"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..637
                     /gene="hprR"
                     /locus_tag="ACHK49_004823"
     CDS             <1..637
                     /gene="hprR"
                     /locus_tag="ACHK49_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310734.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO9265294.1"
                     /translation="QEWVTQGLSEAGYVIDAVSDGRDGLYLALKDDYALIILDIMLPG
                     MDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPFSFSELLARVRAQ
                     LRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLASRAGEIIPRTVI
                     ASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSFVAVKK"
     gene            637..1995
                     /gene="hprS"
                     /locus_tag="ACHK49_004824"
     CDS             637..1995
                     /gene="hprS"
                     /locus_tag="ACHK49_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO9265295.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPATETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLFLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSEGQGLGLSLVKAIAELHGGSATYHYFNKHNVFRITLPQ
                     RN"
     gene            complement(2103..>2830)
                     /gene="hchA"
                     /locus_tag="ACHK49_004825"
     CDS             complement(2103..>2830)
                     /gene="hchA"
                     /locus_tag="ACHK49_004825"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO9265296.1"
                     /translation="KPYRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLH
                     AAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNPKKIADVVVSLNADS
                     EYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLN
                     GYSICAFPDAADKQTPEIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTG
                     DTPFAANALGKLAAQEMLAAYAG"
BASE COUNT          803 a          572 c          665 g          790 t
ORIGIN      
        1 ccaggagtgg gtaacgcagg ggctttccga agcgggttat gtcattgatg ccgtttctga
       61 tggcagagat gggctttatc ttgcgcttaa ggatgattat gcattgatca ttctggatat
      121 tatgcttccg ggtatggatg gctggcagat cttacaaacg ttaagaacag caaagcaaac
      181 tcctgttatt tgccttactg caagagattc tgtcgatgac agagtcagag ggctggacag
      241 tggggcaaat gattatctgg taaaaccttt ttcattttct gagttgctgg caagggttcg
      301 ggcacaatta aggcaacatc acgctttgaa ttcaacatta gaaatcagtg gcttaagaat
      361 ggactctgtt agtcaaagtg tgagcaggga caatatcagt attacactga cgcgcaagga
      421 gtttcagtta ctttggctac tggcctccag agctggcgag attatcccca gaacggttat
      481 tgcgagtgaa atttggggaa tcaactttga tagtgatacc aatacggtgg acgtcgccat
      541 tcgcaggctc cgcgcaaaag ttgatgatcc ttttcctgaa aagctaatcg ccacaatccg
      601 ggggatgggc tattcattcg tagcggtaaa aaaataatga aaagattatc tataaccgtc
      661 cgtttaacct tgctttttat attgctgcta tctgttgctg gcgctggaat tgtatggact
      721 ctctataatg gcctggcaag tgagttgaaa tggcgcgatg atacaacact cattaaccgg
      781 acagcgcaga tcaagcagtt gttaattgat ggggtaaatc cagatacgtt acctgtgtac
      841 tttaaccgga tgatggatgt tagtcaggat atcttgatca ttcatggtga tagcatcaac
      901 aaaattgtta accggacgaa tgtcagtgat gacatgttaa ataacatacc tgctactgag
      961 acaatcagcg cagctggcat ttacagaagc attattaatg atacagagat agatgcttta
     1021 cgaattaata ttgatgaagt ttcgccatca ttaacggtta ctgtggctaa attggcttca
     1081 gccagacata acatgcttga acagtataaa attaatagca ttataatttg cattgtcgcc
     1141 attgtacttt gctcagtatt aagtccgctg ttaatcagaa cgggattacg agagatcaaa
     1201 aagttgagtg gtgtaacgga agcgctgaat tataacgata gccgggagcc tgttgaggtt
     1261 agcgcattac cgagagaact aaaacctctt gggcaggcgt tgaataaaat gcatcatgct
     1321 ttagtcaaag attttgagcg tctaagtcag tttgctgacg atctcgctca tgaacttaga
     1381 acgcccatta atgcattact gggtcagaat caggttacgc tcagtcaaac cagaagtatc
     1441 gctgaatatc aaaaaacaat tgccggtaac attgaagagc tggaaaatat ttcgcggtta
     1501 acagagaaca tactgtttct tgcccgggca gataaaaaca atgttttggt gaaactggac
     1561 tcactttttc tcaataagga agtcgaaaat ttgttggatt atcttgaata cctttcagac
     1621 gagaaagaga tttgctttaa ggtcgagtgc aatcagcaaa tctttgcgga taaaatttta
     1681 ctgcaacgaa tgttatcgaa tcttattgtt aatgccatta gatattcacc agaaaaatcg
     1741 cgcattcata taaccagttt tcttgatacc aacggctatc ttaatattga tatcgccagt
     1801 cctggaacga aaattaatga gcctgaaaaa ctcttccgta gattttggcg gggagataat
     1861 tcgcgtcatt ccgaaggtca gggacttggt ctttctttag tcaaagcgat tgccgaattg
     1921 catgggggaa gtgctacgta tcactatttt aataagcata atgtgttccg aattacgtta
     1981 ccgcaaagaa attaacatag ggcttcagta cgccataaat caaccatgaa aaaacgcagc
     2041 cacgtcgtga gtattaacgt ggctgcgatt gattatgcgc ttacattcat acgtaacagg
     2101 gattaacccg cataagctgc cagcatttcc tgcgccgcca gtttacccaa cgcattcgct
     2161 gcgaaaggag tgtcgccggt gagaacttta cggtccttat gtactcgccc ggtgatgtcg
     2221 tcattaataa tgttcatgcc cattttcttc agctcttcgc cgaagtacca ggtgagatga
     2281 cccggcatat agcctatctc tggcgtttgt ttgtctgcgg cgtctgggaa tgcgcaaatg
     2341 gaataaccat tcagtgggtt atcgccgtgg cgaagcgcca gaaaagccgc cgggccgtgg
     2401 caaagggaga taacaaaacg gtcatttttg attgcccact gcaaagcagc agccacgtcc
     2461 tggctttctg gtaaaccaat aagtgcgcca tgaccaccag gaacaaagat tgctgcatat
     2521 tcgctgtctg cgttgaggct gacgacaaca tccgcgattt ttttcggatt gcggaacaac
     2581 gatttatgct gctcaaagaa tggcattact ttttcatctt tgtgcggcat agcccagtat
     2641 tcaaacttgg tcatcagacc ggaaatggtc gccacttcga attcgaaacc tgcagcatgg
     2701 agatgataca acggcagcaa cgtttcaatc ggatggttac cggtcgagaa cagttttccg
     2761 ttatcggtcg gcaaataacg ttcgtccgcg gcgatcacca gaattttatg tttaccgcga
     2821 tacggttttg
//
LOCUS       DAZGLJ010000346         2829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000346 DAZGLJ010000000
VERSION     DAZGLJ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.350"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            44..676
                     /gene="comR"
                     /locus_tag="ACHK49_004826"
     CDS             44..676
                     /gene="comR"
                     /locus_tag="ACHK49_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO9265297.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(738..1277)
                     /gene="ycfJ"
                     /locus_tag="ACHK49_004827"
     CDS             complement(738..1277)
                     /gene="ycfJ"
                     /locus_tag="ACHK49_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO9265298.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="ACHK49_004828"
     CDS             complement(1504..2808)
                     /gene="ndh"
                     /locus_tag="ACHK49_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO9265299.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          680 a          779 c          690 g          680 t
ORIGIN      
        1 ttataataac ctttcattca cagtcaccag gtacaacggc atcatggcaa ctgactcaac
       61 acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg ccgcgcttga
      121 taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg cggacctcgt
      181 cgaagcgacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca aagaggggtt
      241 atttcgtgcc gttctcgacc gctatatcga tcgttttgcc gctaagcatg aagcacagct
      301 gttttgtgaa gagaaaagcg tggagtctgc gctggctgac tattttgctg ccatcgccaa
      361 ctgctttacc agcaaagaca ccccggcagg ctgcttcatg atcaacaact gcaccaccct
      421 ctccccagat tcaggagata tcgccaatac gttgaaatca cgccatgcga tgcaagagcg
      481 cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga cccactgtga
      541 cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga tcagcgcacg
      601 cgaaggtgca tcgctggaaa aactaatgca gattgccgga acgactttgc gtttatggcc
      661 cgaactggtg aaataagtcg caaaaaaagc ccctcagcct gagcgaatga ggggccaaat
      721 tgcagagtac agccttgtta tactttgtta ttcaaaatca gttgcccatt actatctagc
      781 gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc gccaatctta
      841 taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt acaacgctgt
      901 tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt accggcatat
      961 ccaccaccca gcgcccccac aacagtggcg acatctttac cgcgaccacc gccaaactga
     1021 tgccctatca cgccgccagc aacagcgccg agcaccgacc cggtaatgcg attttcatcc
     1081 tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg tgttttaacc
     1141 gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccacg ttcaaacacg
     1201 ttcagactgg ccactgccgc tacgcccagc gcagctgcga caccaatccc gatacccgcc
     1261 aacattgatt tattcacggg aacacctcct tctttgcagt tatgccaatc atgcaagcga
     1321 aggagaaaag cagctatcag acagaagatg aaaagcgcgg gaatggctgg aaatacttaa
     1381 tgattcggag gagtctgaag gaatgcctga tgcgacgctt aacgcgtctt atcaggccta
     1441 ccgctcgtac catttgtcca gggcggatga ggcgtttatg ccacatccgc cagtgtacgt
     1501 cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca ccagcatcat
     1561 taatccggtt ttaaagtaac catgcagcgc aatctgatgc attcggtata gcgagatata
     1621 tacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt tacccatcag
     1681 gctaccaacg gtggagaagt tcgacagcga taccagcgaa ccatgatctt tatactgata
     1741 atttttcagc ggcttaccat tcatctgcgc cagaatgttg ttcattgcgc aagtcgccat
     1801 ctggtgtgca gcctgagcac gcggcggaac aaagccccct tccggacgcg ggcatgacgc
     1861 gcagtcgcca atagcgtaaa tgtctggatc gcgggtggtt tgcagcgtcg gttccaccac
     1921 cagctggttg atacggttag tttcaagacc accgatatct ttcaggaagt ctggcgcttt
     1981 gatcccggct gcccacacca tcagatcagc ctcaatatat tcgccatctt tagtgtgcag
     2041 gccgccttca tcagcactgg tgaccatggt ttgcgtcagc acgcgaacgc caagtttcgt
     2101 tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa tacgttctcc
     2161 cgcttctacc agcgttacgt tcagggcttc gttggtcagg cctttgtaac cgtagctgtg
     2221 cagttgcttg accgcgttgt gcaattcagc ggagagttct acacccgtcg cgccgccgcc
     2281 gacaatcgca atgttcactt tgccatttgc gcccaggttg gcggagtatt tcaggaagag
     2341 attcagcatc tcctggtgaa agcgacgcgc ctggtgcggg ttatcgagga aaatgcagtt
     2401 ctctttgaca cctggcgtat tgaaatcgtt agaggtgcta cccagcgcca ttaccagggt
     2461 gtcataggcg attttacgtt ccggaaccag cagttcacct ttctcatcgc gcagttctgc
     2521 aatagtgatt gttttcgctt cacgatcaat atcaatgacg gaacccagct ggaactggaa
     2581 accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat caagcgagcc
     2641 agtcgccact tcgtgcagca gcggtttcca cagatggctg tggttacgat cgaccagcgt
     2701 aattttggct tttttcttgc gtcccagctt atgccccagc tgtgttgcca tttccagccc
     2761 accagcaccg ccgccgacaa tcacaatctt tttcaatggc gtagtcaacg tgaccccctt
     2821 aaatttatt
//
LOCUS       DAZGLJ010000347         2799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000347 DAZGLJ010000000
VERSION     DAZGLJ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.351"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            109..2388
                     /gene="ydeP"
                     /locus_tag="ACHK49_004829"
     CDS             109..2388
                     /gene="ydeP"
                     /locus_tag="ACHK49_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO9265300.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLSWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKWMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2636..>2799
                     /gene="safA"
                     /locus_tag="ACHK49_004830"
     CDS             2636..>2799
                     /gene="safA"
                     /locus_tag="ACHK49_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO9265301.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDL"
BASE COUNT          738 a          658 c          682 g          721 t
ORIGIN      
        1 agtagtcccc tactcttata tatccatgtt ggcgataatc cctttgtatg tactgtgcat
       61 catcgctatt acaaatccta ataattcatt tccacacagg ataagtagat gaagaaaaaa
      121 attgaatcct accagggtgc tgcaggtggt tggggtgctg ttaaatccgt agcgaatgca
      181 gtacgtaagc agatggatat acgccaggat gttattgcca tgtttgacat gaataagcca
      241 gagggctttg actgtccggg ttgtgcatgg ccagatccta agcacagtgc gtcattcgac
      301 atttgtgaaa acggcgcaaa agcaatcgcc tgggaagtca cggataagca ggtaaatgcc
      361 tctttctttg ctgagaatac ggttcaatca ttactttcct ggggagatca cgagcttgag
      421 gctgcgggac gtctcactca gcctttgaaa tatgatgccg tcagcgactg ttacaagcca
      481 ttaagctggc aacaagcttt cgacgaaatt ggcgcacgcc ttcaaagcta tagtgatccc
      541 aatcaggttg aattctatac ttcgggccgc acttccaatg aagctgcctt tctttatcag
      601 ctttttgccc gtgaatacgg gagcaataac tttcccgact gctccaacat gtgccatgaa
      661 ccgacaagcg tgggtttggc agcgagtatc ggtgtaggta aagggaccgt gttgctggaa
      721 gactttgaga agtgcgattt agtcatttgc attgggcata accctggtac aaaccaccct
      781 cgcatgctga cttcgttgcg cgctttagtg aaacggggag cgaaaatgat cgccatcaat
      841 cctctacagg aacgtggcct ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg
      901 acgaactctg agactcagtt ggccagtgcc tactacaacg tgcgcattgg tggcgatatg
      961 gcgttgctca agtggatgat gcgcctgtta attgagcgcg atgatgctgc aagtgccgca
     1021 ggtcggccct cattgcttga tgacgaattt attcaaacgc ataccgtcgg ctttgacgag
     1081 ctacgccgtg acgttctcaa ttccgagtgg aaagatatcg aacgtatttc tggactaagt
     1141 cagacacaaa tcgccgaact ggctgacgca tatgccgctg ccgaacgcac cattatctgt
     1201 tacggaatgg ggatcactca gcacgaacat ggtacccaga acgtacagca actggtcaat
     1261 ttgctgttga tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc actacgtggt
     1321 cactctaatg tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt
     1381 ctggctcgcc tgggtgagcg ctatggcttc accccacctc atgcacctgg acatgctgca
     1441 attgccagca tgcaagcaat atgtacgggg caggctcgag cattgatctg catggggggc
     1501 aactttgcac tggcaatgcc agatcgggaa gcgagcgctg taccgttaac gcaattagat
     1561 ttggcggtac acgtagccac taagcttaac cgctctcatc tgctgaccgc acggcatagc
     1621 tatatcctgc cggttctggg gcgtagcgag attgatatgc aaaaaagcgg tgcgcaggcg
     1681 gtaaccgttg aggattcaat gtcgatgatt catgcctcgc gtggcgtgtt aaaacccgcc
     1741 ggtgtaatgc tgaaatcaga gtgtgctgtg gtcgcgggaa tcgcccaggc aacactaccc
     1801 cagagcgtgg tagcctggga gtatctggtg gaagattatg atcgcattcg caatgacatt
     1861 gaagctgtgc tgccagagtt cgccgactat aaccagcgca tccgtcatcc cggtggtttt
     1921 cacctgataa atgcagctgc tgaaaggcgc tggatgacgc cgtcaggtaa ggctaatttc
     1981 attaccagca aagggctgtt agaagatccc tcttcagcgt ttaacagtaa gctggtcatg
     2041 gcgacagtac gcagccacga tcagtacaac acgacgattt atggtatgga tgatcgctat
     2101 cgaggggtat tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc taaaatttgc
     2161 cgtgtaaaaa acggcgaaag agttaatctt attgcgctta cgccagacgg taagcgcagc
     2221 tcacgccgca tggatagatt aaaagtggtc atttacccta tggctgaccg ctcactggtg
     2281 acctattttc cagaatcgaa tcacatgcta actcttgata accacgatcc attaagtggc
     2341 attcctggtt ataaaagtat tccggttgaa ttagaaccat caaattaatt cgcctcctca
     2401 tttcttctgc tgtcatccgc acagcagaag aatttctcat tgactattat cgcgcaattt
     2461 gctcacattg attaaattaa accacacact ataagatata aacctctgcc tacagctgta
     2521 agaaactccg ctcagtactg aagtgccagt cctatttcct cttttctcca gcctgttata
     2581 ttaagcatac tgattaacga tttttaacgt tatccgctaa ataaacatat ttgaaatgca
     2641 tgcgaccaca gtgaaaaaca aaatcacgca aagagacaac tataaagaaa tcatgtctgt
     2701 aattgtggtt gtcttattac tgacccttac gttgatagcc attttttcgg caattgatca
     2761 gctgggtatt tcagaaatgg gtcgcattgc aagagatct
//
LOCUS       DAZGLJ010000348         2743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000348 DAZGLJ010000000
VERSION     DAZGLJ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.352"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(110..202)
                     /locus_tag="ACHK49_004831"
                     /pseudo
     CDS             complement(110..202)
                     /locus_tag="ACHK49_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219338.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
     gene            complement(235..573)
                     /locus_tag="ACHK49_004832"
     CDS             complement(235..573)
                     /locus_tag="ACHK49_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9265302.1"
                     /translation="MLMVKKTIYVNPDRGQNRKVSDRGLTSRDRRRIARWEKRIAYAL
                     KNGVTPGFNAIDDGPEYKINEDPMDKVDKALATPFPRDVEKIEDEKYEDVMHRVVNHA
                     HQRNPNKKWS"
     gene            complement(576..881)
                     /locus_tag="ACHK49_004833"
     CDS             complement(576..881)
                     /locus_tag="ACHK49_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFO9265303.1"
                     /translation="MVTIVWKESKGTAKSRYKARRAELIAERRSNEALARKIALKLSG
                     CVRADKAASLGSLCCKKKEEVVRKNRSIYYKDSNPLGNKIHAVQKIKLYSKLPYGAY"
     gene            complement(1196..1846)
                     /locus_tag="ACHK49_004834"
     CDS             complement(1196..1846)
                     /locus_tag="ACHK49_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO9265304.1"
                     /translation="MNTQLMGERIRARRKELKIRQAALGKMVGVSNVAISQWERSETE
                     PNGENLLALAKALQCSPDYLLKGEDSLSNIAYHSRHDPRGSYPLISWVSAGCWMEAVE
                     PYHRRAIDNWYDTTVDCSEDSFWLDVKGDSMTAPAGLSIPEGMIILVDPEVEPRNGKL
                     VVAKLEGENEATFKKLVIDAGRKFLKPLNPQYPMIEINGNCKIIGVVVDAKLANLP"
     gene            1927..2112
                     /locus_tag="ACHK49_004835"
     CDS             1927..2112
                     /locus_tag="ACHK49_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO9265305.1"
                     /translation="MYKKDVIDHFGTQRAVAKALGISDAAVSQWKEVIPEKDAYRLEI
                     VTAGALKYQESAYRQAA"
     gene            2222..2518
                     /locus_tag="ACHK49_004836"
     CDS             2222..2518
                     /locus_tag="ACHK49_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO9265306.1"
                     /translation="MAQASYSKLTQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPVAAGDSKQIDMQF"
     gene            2551..>2743
                     /locus_tag="ACHK49_004837"
     CDS             2551..>2743
                     /locus_tag="ACHK49_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO9265307.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLIEAYSGAD
                     LTKRQFKVLLAILRKTYGWN"
BASE COUNT          717 a          619 c          570 g          837 t
ORIGIN      
        1 atcccatctt cttcaactgt cgcctggaat ctccagaaaa caggcattcc atcatgttca
       61 gtttctgatt caggaaaagg tacgctccat gattttgtca tatctcacct caaataagtg
      121 gtttgctgcc taatttcatt ttctggcgac caacacaagt cacacccatt tcactgcgtg
      181 gcttgctgta ataaattagg ttagttcagc caataaaaaa cccaccgaag tgggctatga
      241 ccatttttta tttggatttc gttggtgagc gtgattaaca actctgtgca ttacatcctc
      301 atatttttca tcttcaattt tttcgacatc gcgaggaaat ggtgttgcta atgctttgtc
      361 aactttgtcc attgggtctt cattaatctt atattcagga ccgtcatcta tagcattaaa
      421 tccaggtgtt acaccgtttt ttaatgcata tgctatcctc ttttcccatc tcgctattct
      481 cctcctgtct cgagatgtaa gacctctatc agatactttt ctgttttgtc cgcggtcagg
      541 attaacataa atagtctttt tcaccataag catactcaat aagcaccgta cggtagttta
      601 ctgtacaatt ttattttttg gactgcatgt attttgtttc ctaatgggtt tgaatccttg
      661 taataaatac ttctattttt tcgaacaact tcttctttct tcttgcagca aaggcttcct
      721 agtgatgctg ctttgtctgc tctgacgcaa ccagagagct ttagcgcaat ttttcgcgcc
      781 agtgcttcat tactgcgtcg ctcggcaata agttctgctc tgcgagcttt gtagcggctt
      841 tttgccgtac ctttggattc tttccagaca atggttacca tgatggtctc ctttaagtgg
      901 ctttggcgca tgacgcgtcg aggtgcttat cttctcgatc gctgtcttgc agctgcaatt
      961 cgcgccatcc ccaaaaccac tcaagttctg gtctcaacgg ttaggttgag agttcgtcga
     1021 tgttaaagag cctgccaatc tgttccgttt ggcttccagc ctcctgctga cggcttaaaa
     1081 ttaagacctc ttaatttttt ggtcaagtgc atttttgaag aaaacttaat tttatgggcg
     1141 cgaatttagt ttgtctttga tttttaacgg gaaataaaaa aggggcgaaa gccccttaag
     1201 gaaggtttgc tagcttggca tcaacgacaa cgccaatgat tttacagttc ccattgattt
     1261 caatcattgg gtattgtgga ttgagtggtt tcaggaattt tctaccggca tcaataacta
     1321 actttttgaa tgtcgcctcg ttttctcctt caagtttggc gactaccagc tttccattac
     1381 gtggttcgac ttctgggtcg acgagaataa tcatcccttc aggaatactc agtcctgccg
     1441 gggcagtcat tgaatcacct ttaacgtcga gccaaaaaga gtcttcagaa caatctaccg
     1501 ttgtgtcgta ccagttatct attgcacgcc tatgatatgg ctctacagct tccatccaac
     1561 atcctgcgct tacccaacta attagaggat acgaacctct tggatcatgc ctgctgtgat
     1621 aggcaatgtt tgaaagacta tcctctcctt tcaacaggta atcaggggag cactgcaaag
     1681 ccttggctaa ggccaatagg ttttcgccat tgggctcagt ttcagatcgc tcccattggg
     1741 aaatagcaac attagacacg ccaaccatct tgccaagggc agcctgccta atcttgagtt
     1801 cttttctgcg agcgcgaata cgctcaccca tcagttgtgt attcatagtt aagacatctt
     1861 aaataaactt gacttaagat tcctttggtg gataatttaa gtgttcttta atttcggagc
     1921 gagtctatgt acaaaaaaga tgttattgac cacttcggaa cccagcgtgc tgttgctaaa
     1981 gcactaggca ttagcgatgc agcagtctct cagtggaaag aagttatccc agagaaagac
     2041 gcctatcgat tggaaatcgt tacagctggc gccctgaagt atcaagaaag tgcttaccgc
     2101 caagcggcat aagtaaattg ctctttaaca gttctggcct ttcacctcta accgggtgag
     2161 caaacatcag cggcaaatcc actgggtgtg ccgctatata tcaatatagg aaaattaaca
     2221 aatggcacaa gcaagttaca gcaagctaac acagcgagaa attgatcgcg cagaaacaga
     2281 tttactcatc aacctgtcaa cgcttaccca gcgcggtctg gcaaagatga ttggctgtca
     2341 tgaatcgaag ataagcagaa cggactggag atttattgct tcggtcttgt gtgctttcgg
     2401 aatggcatca gacatcagtc cgattagcag ggcttttaag tatgcgcttg atgaaatcac
     2461 aaagaaaaaa tccccggtgg ccgccgggga ctctaagcaa attgatatgc aattctgagg
     2521 gaattactgg atcaatccac aggagtcatt atgacaaata cagcaaaaat actcaacttc
     2581 ggcagaggta actttgccga acaggagcgt aatgtggcag atctcgatga tggttacgcc
     2641 agactatcaa atatgctgat tgaggcttat tcaggcgcag atctgaccaa gcgacagttt
     2701 aaagtgctgc ttgccattct gcgtaaaacc tatgggtgga ata
//
LOCUS       DAZGLJ010000349         2737 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000349 DAZGLJ010000000
VERSION     DAZGLJ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.353"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(65..823)
                     /gene="yhdZ"
                     /locus_tag="ACHK49_004838"
     CDS             complement(65..823)
                     /gene="yhdZ"
                     /locus_tag="ACHK49_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO9265308.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(831..1934)
                     /gene="yhdY"
                     /locus_tag="ACHK49_004839"
     CDS             complement(831..1934)
                     /gene="yhdY"
                     /locus_tag="ACHK49_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO9265309.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1944..>2737)
                     /locus_tag="ACHK49_004840"
     CDS             complement(1944..>2737)
                     /locus_tag="ACHK49_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO9265310.1"
                     /translation="FRNIPPLLQIFFWYFAVLRNLPGPRQAVSAFDLAFLSNRGLYIP
                     SPQLGDGFIAFILAVVMAIILSVGLFRFNKTYQIKTGQLRRTWPIAAVLIIGLPLLAQ
                     WLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQAVPYG
                     QHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAAIGYPDMVSLF
                     AGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          755 a          733 c          649 g          600 t
ORIGIN      
        1 catcaaatca agcaaaaagg ccatccgaaa ggatggcctt tttgcttttc gaactaacat
       61 tcaattaatg gattacctgc gataaaaatg ccctcgtacg ctctgattta ggatgcgtaa
      121 aaaattcatc aggtgcagct tgctccacta tttccccacg atccataaaa atcacccggt
      181 cagcgacggt tcgcgcaaac cccatctcat gcgttacaca caacattgtc atacccgatt
      241 gcgccagccc aatcatcgta tccagcacct ctttcaccat ctccggatcg agcgccgacg
      301 ttggctcatc aaacaacata attttcggct tcatacacag cgaacgcgca atggcaacgc
      361 gttgctgctg accaccagaa atttgtccgg gaaacttatg cgcatgttcg gcaattctca
      421 cccgctcaag gtaatgcatc gccagagctt cagcctcttt ctttggcatc ttgcgtaccc
      481 aaatcggtgc cagggtacag ttctgtagaa cggtcagatg agggaagaga ttgaaatgct
      541 gaaagaccat tcccacttcc tgccggacgc gctcaatatt gcggatatct tcattaagtt
      601 cgatgccatc taccacgatc cgtccctgtt gatgttcttc cagatgatta atacaacgaa
      661 tggttgtcga tttaccggaa cctgaagggc cacacagaac gatccgttct cccggttgca
      721 cggttaaatt tatatttttc aaaacatgga attgtccata ccatttattg acgttttcca
      781 gcgtaatcat cgcgttagca ggttgcagta aaatttggct catagtgtcc tcaatgcggt
      841 gtacgcccgg tgttaaaacg tttttccaga tgctggctat agcgcgacat gctgaaacag
      901 aagatccagt agatcagcgc ggcgaaaaca tacccttccg tcgacatacc caaccaggtg
      961 ggatcaacgg ttgcctgctg aacgctactg aaaagatcga acaacccgat aatgatcacc
     1021 aggctggtat ctttgaagag tgcgatgatg gtatttacca gcccaggaat taccagcttc
     1081 aacgcctgtg gcagaataac cagcccctga gttttccagt aacccaacgc cagcgactct
     1141 gccgcttcat actgcccttt aggcagtgcc tgtaatccgc ctcgcacgac ttccgcaaca
     1201 tatgctgact gaaacaggat cacgccaacc agcgcgcgga tcagtttgtc gatactggtg
     1261 ccttctgcca taaacaacgg cagcatgacc gaagacataa acaatacggt aatcagcggt
     1321 acgccgcgcc agaactcgat aaaaatgacc gataagatac gcacaatcgg catatgggag
     1381 cggcgaccta acgccagtaa gatcccccac ggtagcgccc ccgcaatccc aacggatgca
     1441 ataattaaag ttagcgtcag cccgccccat tgccgggttt caacccgctc cagaccaaaa
     1501 aaaccgccat acatcagcca ccagacaatc agtgggtaaa tcaccgccca gaccgcaata
     1561 tagcgaccgc gatgcgggag tattttccag aacattggtg cgattgacac aaggccaatc
     1621 agtaatgcca ggttaattcg ccagcgttgg tcgtgtgggt aaagtccata cataaactga
     1681 ccaaatcgtt cgtggatgaa gacccaacag gcaccggctt ttgtgcagtc ggcacgcgtt
     1741 gagccaaccc aattagcctg caaaaatgcc cagttcagca acggtgggat cagttcccac
     1801 atcaaccata tgcagccaat agtcagcagg ctattgctcc agctggagaa cagatttttt
     1861 cgcacccaca ccatcgcgcg gcttgagtta tggctcgctg ggcgcgaggg ctgagacagc
     1921 aatacttttg tcatcatgac tccttagcgt tcaacgatcg cgatgcggcg gttatagata
     1981 ttcatcagca gcgagatagt caggctgata atcagataga ccgacatggt catggctatc
     2041 gtctcgatgg cttgccccgt ctggttcagc acggtgccag caaacagcga aaccatatcg
     2101 ggatagccaa tagcggcggc aagagaggag tttttgacga tgttgagata ctggctggtt
     2161 aacggcggaa taatcactcg cagtgcctgg ggaataatga cctggcgtag cgtaaccggg
     2221 ttgggtaatc ccagtgaccg agccgcttca tgttgaccat aaggcactgc ctggatccca
     2281 gcgcggataa tctcggcgat aaatgcagat gtataaaccg aaagtgccag cgttaaggct
     2341 gccagttcag gaattaaaac catcccgccg cggaagttaa agcctcgtag ggctgggaca
     2401 tcccagtgta gtgctgcgcc aaaaagccat tgcgccagta aaggcaaacc aatgatcaac
     2461 actgcggcga tcggccaggt gcggcgcagt tgtccggtct ttatctggta tgttttatta
     2521 aagcggaata gcccaacaga aaggattata gccataacaa cagccaggat aaacgcaata
     2581 aatccgtctc ccagctgcgg tgacggaata taaagcccac gattgctcaa aaaggccaga
     2641 tcaaacgcgc tcacagcctg gcgtggtccg ggcaaattgc gcaacacggc aaagtaccag
     2701 aagaagattt gcagcagcgg gggaatatta cggaaga
//
LOCUS       DAZGLJ010000350         2725 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000350 DAZGLJ010000000
VERSION     DAZGLJ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.354"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>2725)
                     /locus_tag="ACHK49_004841"
     CDS             complement(<1..>2725)
                     /locus_tag="ACHK49_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9265311.1"
                     /translation="PRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTAL
                     VGVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWC
                     LWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTELRITCNAYLTTQR
                     KAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSA
                     LKDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGL
                     WLIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTL
                     DREITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPT
                     LRQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQH
                     LTAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFR
                     QLALGRYTLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYD
                     PTVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYICSVNTVGK
                     SAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVS
                     NEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIE
                     NTSDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGN
                     PPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCRVLGKLSANQIEGDLVKTV
                     GKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEREHTDI"
BASE COUNT          536 a          853 c          706 g          630 t
ORIGIN      
        1 aaatatcagt atgctctctc tcatgtttag cgccgctgaa tgccaccgcc ggaataacaa
       61 tctgccggtc aaacggctga tcgtcataaa ccctgacggt aatggtccct gatggccacc
      121 gttccggtgc acgggagtcc cgggggaaag ctttgcccac tgttttaacg agatcgcctt
      181 caatctggtt cgcggacagt tttcccagaa cccgacagtt ctcgttaatc gtgacattgt
      241 tgagcgtccc ggcgttcgca ttcacactgc cactgatatc cgcattttta gcggtcagct
      301 ttccgtccgg tgtcagggaa aatgccggtg gatttccacc gctggtaatg gtgggggccg
      361 tcaggcgttt caggaacacg tcgttcatga atatctgatc gccctgacca acaaacatcg
      421 gttttgtgtt gccattcgca ggattaacca tcgcaatcct gtccgccgcc agcagcacct
      481 gactctgcat gccgtcagag gtgttctcaa taccggcacc aatacccgcg atataaaggc
      541 gtccgtcctg catctgctgc agcttcacag cccacatgct gttcaggtta ttatttgtat
      601 caacctgaac cttctgtatc tgctgaattg ccgcactctg gtcttccagt ttcttattga
      661 cggtctgcgt gatttcattg ctgacatccg taatggacgt cctgatttca gccaggtcag
      721 gcgcaagctg accgttatca atctgcgtcc acaactcctg agccagatgg gttttcccta
      781 tctcgccttt gaaaaaatcc agatagccgg atgcatcatc actcggctgg ccgacggcct
      841 ccacaaatgc cgatttgcca acggtgttca cactgcagat gtaaaaataa taatcatggc
      901 ccggcttaat attgatactg gcagctatcc agtacagcgc cgtgccaaga tagcgggctg
      961 tggtttcaac ctgcctgata tccgcaatcc gcttttccga gaaccagaat tcaaactgta
     1021 ccgtcggatc ataaaccgca agatgcggcg tggcggttat ctgaaaatag cccggcgtca
     1081 gctcaatccg cgacggcgct gccggtgcgg caatccggaa cgataccgac gccggatcgc
     1141 cctgctgtcc ccacgcattt accgcccgga ctgtcagcgt gtaacgcccc agcgccagtt
     1201 gcctgaagcg gtatgtggtt tccgtcgtcc gggccgtgct gaccagccgc tcactgccgt
     1261 catccgctgc cacggtcagg cgaagaagga agctaacgcc cttcaccacc ttcggcgtgt
     1321 cccagcgcgc cagcacctga tattccccgc tgtctgcggt gacttcggca gtcaggtgct
     1381 gtaccgctgg cggcgtgaca ccattcaccg tgccgctctg gtcgccgtca aagtgcgccc
     1441 cgttatccac gatggcttct ttttccggta catgctgcac ggcagtgatg gcatacgtgc
     1501 cgtcatcgtt ctcacggata ctcacgcagc ggaacaggcg ctggcgcagc gtcggcaact
     1561 tcagccccca tacgctgtat tcagcaacgc cgtcaggaac acggctcact ttcaccttca
     1621 cgccgtcggt gacggactga acctccacgc tgaccggatt gccacttccg tcaaccaggc
     1681 ttatcagcgt ggtaccggag gatggcagcg tgatttcacg gtcgagcgtc agcgtccggg
     1741 tctggctgtt caccgccagc acgcgcccgc cggtgctgat accggcatag tcatcatcgc
     1801 agatttcaat gacatcgccc ggcacatggc gaagcccttc tgcgcccacg ctgaagtcca
     1861 cggtctgcgt ttccagcagt tctgttttaa tcagccacag cccggcgcgg tgtgcctgcc
     1921 cccggctggt acagccaaaa gcatccatct tcgtgacgtt acgaccgtaa cgggcaatgg
     1981 cctgcgtatc ctccacaagc tctgtcgccg tctcccagcc gttgttcggg tcaatccagt
     2041 tcacctcaac ggcattatgg cggtccttca gggcgctgaa gctgtagcgg aacggcgcgc
     2101 catcatccgg catcaccaca ttactgcggt tataggtcca caccttatcc gacggtcggt
     2161 cctgcacgaa cgtcagcgtc tgcccgttcc ataccggcat acagcgcatc gccgagcaga
     2221 aatcactgag cacatcccac gccttgcgct gtgtggtcag gtacgcatta caggtgatgc
     2281 gcagctccgt gccgccaaag ccgtccggca ctgactggtc gcagtactgg ccgatgacat
     2341 acagcgccca tttgtccaca tccgccgcac caagacgttt ccccatgccg tagcgcgggt
     2401 gggtcagcat atcccacaga caccaggcca tgttgttgct gtatgctggt tttaacgttc
     2461 cgtcccagat accgctgtat tgtcgcgtct gcgggttata gttcgacggc acctgcagaa
     2521 tgcgcccgcg aagatgataa ttacgactca cctgctggct gccgaactgc tccgagtcca
     2581 cctgtacgcc gaccagtgcc gtgttcgggt agcactgttt cacatcgatg atttcggtgt
     2641 atgacgacca gagcgttttg ttctgcagct ggtctgtggt gctgtccggc gtcatcctgc
     2701 gcatccggat attaaacggg cgcgg
//
LOCUS       DAZGLJ010000351         2697 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000351 DAZGLJ010000000
VERSION     DAZGLJ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.355"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..192
                     /gene="gpU"
                     /locus_tag="ACHK49_004842"
     CDS             <1..192
                     /gene="gpU"
                     /locus_tag="ACHK49_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO9265312.1"
                     /translation="PAQVPDSELDAWMESRIYPVMSDIPALSELITSMVASGYDYRRD
                     DDAGLWSSADLTYVITYEM"
     gene            200..940
                     /locus_tag="ACHK49_004843"
     CDS             200..940
                     /locus_tag="ACHK49_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9265313.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATDMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTDS"
     gene            956..1378
                     /locus_tag="ACHK49_004844"
     CDS             956..1378
                     /locus_tag="ACHK49_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO9265314.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKQPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            1360..1794
                     /locus_tag="ACHK49_004845"
     CDS             1360..1794
                     /locus_tag="ACHK49_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO9265315.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            1787..>2697
                     /locus_tag="ACHK49_004846"
     CDS             1787..>2697
                     /locus_tag="ACHK49_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO9265316.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARA"
BASE COUNT          592 a          690 c          876 g          539 t
ORIGIN      
        1 cctgctcagg tgccggattc agagctggat gcgtggatgg agtcccggat ttatccggtg
       61 atgagcgata tcccggcact gtcagagttg atcaccagta tggtggccag tggctatgac
      121 taccgacgcg acgatgatgc gggcctgtgg agttcagccg atctgactta tgtcattacc
      181 tatgaaatgt gaggacgcta tgcctgtacc aaatcctgta atgccggtga aaggtgccgg
      241 gaccaccctg tgggtttata aggggagcgg tgacccttat gcgaatccgc tttcagacgt
      301 tgactggtcg cgtctggcaa aagttaaaga cctgacgccc ggcgaactga ccgctgagtc
      361 ctatgacgac agctatctcg atgatgaaga tgcggactgg actgcgaccg ggcaggggca
      421 gaaatcagcc ggagatacca gcttcacgct ggcgtggatg cccggagagc aggggcagca
      481 ggcgctgctg gcgtggttta atgaaggtga tacccgtgcc tataaaatcc gcttcccgaa
      541 cggcacggtc gatgtgttcc gtggctgggt cagcagtatc ggtaaagcgg tgacggcgaa
      601 ggaagtgatc acccgcacgg tgaaggtcac caatgtggga cgcccgtcga tggcagaaga
      661 tcgcagcacg gtcacagcgg caaccgacat gaccgtgacg cctgccagca cctcggtggt
      721 gaaagggcag agcaccacgc tgaccgtggc attccagccg gaaggcgcaa ccgacaagag
      781 cttccgtgcg gtgtctgcgg ataaaacaaa agccaccgtg tcggtcagtg gtatgaccat
      841 caccgtgaaa ggtgttgctg caggcaaggt caacattccg gtcgtatccg gtaatggtga
      901 acttgctgcg gttgcagaaa tcactgtcac cgacagttaa tccggagagt cagcgatgtt
      961 cctgaaaacc gaatcatttg aacataacgg cgtgaccgtc acgctttctg aactttcagc
     1021 cctgcagcgt attgagcatc tcgccctgat gaaacggcag gcagaacagg cggagtcaga
     1081 cagcaaccgg aagtttacag tggaagacgc catcagaacc ggtgcttttg tggtggcgat
     1141 gtccctgtgg cataaccatc cgcagaagac aaagcagcct tccatgaatg aagccgttaa
     1201 acagattgag caggaagtgc ttaccacctg gcccacagag gcaatttctc atgctgaaaa
     1261 cgtggtgtac cggctgtccg gtatgtatga gtttgtggtg aatgatgccc ctgaacaggc
     1321 agaggacgcc gggcctgcag agcctgtttc tgcgggaaag tgttcgacgg tgagctgagt
     1381 tttgccctga aactggcgcg cgagatgggg cgacccgact ggcgtgccat gcttgccggg
     1441 atgtcatcca cggagtatgc cgactggcac cgcttttaca gtacccatta ttttcatgat
     1501 gttctgctgg atatgcactt ttccgggctg acgtacaccg tgctcagcct gtttttcagc
     1561 gatccggata tgcatccgct ggatttcagt ctgctgaacc ggcgtgaggc tgacgaagag
     1621 cctgaagatg atgtgctgat gcagaaagcg gcagggcttg ccggaggcgt ccgctttggc
     1681 ccggacggga atgaagttat ccccgcttcc ccggatgtgg cggacatgac ggaggatgac
     1741 gtaatgctga tgacagtatc agaagggatc gcaggaggag tccggtatgg ctgaaccggt
     1801 aggcgatctg gtcgttgatt taagtctgga tgcggccaga tttgacgagc agatggccag
     1861 agtcaggcgt catttttccg gtacggaaag tgatgcgaaa aaaacagcgg cagtcgttga
     1921 acagtcgctg agccgacagg cgctggctgc acagaaagcg gggatttccg tcgggcagta
     1981 taaagccgcc atgcgtatgc tgcctgcaca gttcaccgac gtggccacgc agcttgcagg
     2041 cgggcaaagt ccgtggctga tcctgctgca acaggggggg caggtgaagg actccttcgg
     2101 cgggatgatc cccatgttca gggggcttgc cggtgcgatc accctgccga tggtgggggc
     2161 cacctcgctg gcggtggcga ccggtgcgct ggcgtatgcc tggtatcagg gcaactcaac
     2221 cctgtccgat ttcaacaaaa cgctggtcct ttccggcaat caggcgggac tgacggcaga
     2281 tcgtatgctg gtcctgtcca gagccgggca ggcggcaggg ctgacgttta accagaccag
     2341 cgagtcactc agcgcactgg ttaaggcggg ggtaagcggt gaggctcaga ttgcgtccat
     2401 cagccagagt gtggcgcgtt tctcctctgc atccggcgtg gaggtggaca aggtcgctga
     2461 agccttcggg aagctgacca cagacccgac gtcggggctg acggcgatgg cacgccagtt
     2521 ccataacgtg acggcggagc agattgcgta tgttgctcag ttgcagcgtt ccggcgatga
     2581 agccggggca ttgcaggcgg cgaacgaggc cgcaacgaaa gggtttgatg accagacccg
     2641 ccgcctgaaa gagaacatgg gcacgctgga gacctgggca gacaggactg cgcgggc
//
LOCUS       DAZGLJ010000352         2670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000352 DAZGLJ010000000
VERSION     DAZGLJ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.356"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..908
                     /locus_tag="ACHK49_004847"
     CDS             <1..908
                     /locus_tag="ACHK49_004847"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HFO9265317.1"
                     /translation="KPTGPDNSDVLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLL
                     NDENNELSDDTNFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLE
                     DALNENIKSDPSWPNEVQLFHINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALG
                     HDKENSLKRVINSFNSELMGEMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMA
                     AQEVIEQRESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDAR
                     INASYTQLEIKHSQPKNRASGKRVS"
     gene            complement(979..>2670)
                     /locus_tag="ACHK49_004848"
     CDS             complement(979..>2670)
                     /locus_tag="ACHK49_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HFO9265318.1"
                     /translation="ESENKESQQQQPSTPTDQQVLAAQQAAIKEAEQRSVADKATADT
                     KAKALAQQEAQQYSDKQALQGRLQAAPKYQHAAREKAASQIANPGTARYKQFDDNPVK
                     QVAQNPLATFSLDVDTGSYANVRRFLNHGLLPPPDAVRVEEIVNYFPYDWDIKDKQSI
                     PATKPIPFAMRYELAPAPWNEQLTLLKIDILAKDHKSEELPASNLVFLIDTSGSMISD
                     ERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISGSHKAEINAAIDSLDAEG
                     STNGGAGLELAYQQAAKGFIKGGINRILLATDGDFNVGIDDPKSIESMVKKQRESGVT
                     LSTFGVGNSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLNSEMRQTLITVAKDVKAQ
                     IEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIGAGKHITLLFELTLNGQKASID
                     KLRYAPDNKSVKSDKTKELAWLKIRWKYPQGKESQLVEFPLGPTINAPSEDMRFCAAV
                     AAYGQKLRGSEYLNNTSWQQIKQWAQQAKGEDPQGYRAEFIRLIELADGVTDISQ"
BASE COUNT          738 a          615 c          550 g          767 t
ORIGIN      
        1 ttaaaccaac tggtccggat aattcagatg ttctcctgtt caaaaagcta ttctcccctt
       61 cgaaattaat gacaattatc ggtgatgaaa ttcctcttat atctgaaaaa cagtcgcttt
      121 caaaggtgct tttaaatgat gagaataatg aactgagtga tgatacaaac ttctgggata
      181 aaaatcgtca attaaccaca gatgaaatag cttgctatct tcagaagatc gccgctaatg
      241 caaaaaatac tcaagtcaat tatcctactg gtctctacgt cccctactcc accagaactc
      301 acctggaaga cgctctcaat gaaaatatta agagcgatcc atcatggccg aatgaagtcc
      361 agttattcca cataaatact ggcggacact ggatattagt ttcgctacag aaaatagtaa
      421 ataaaaaaaa taataaacta caaataaaat gcgtcatatt caactcattg cgtgcactag
      481 gccatgataa agaaaattca cttaagcgtg tcattaatag ttttaattct gaactcatgg
      541 gagaaatgtc gaataataat ataaaagttc atttaaatga accagagata atatttttac
      601 atgccgatct tcagcaatac ttaagccaaa gttgcggtgc gtttgtgtgc atggcagccc
      661 aggaagtgat tgaacaaagg gaaagcaatt ctgacagcgc cccctatacg ttattaaaaa
      721 actatgctga cagatttaaa aaatattcag cagaagagca gtacgaaatt gattttcaac
      781 atcgactggt aaacagaaat tgttatttag ataaatatgg cgatgcacga atcaatgctt
      841 cttatacaca attagaaata aaacactcac aacccaaaaa tagagcatcc ggcaaaagag
      901 tgagttaaca tcattgttaa tcaattcgac cagtcagcaa atatgctgac tggtcgaaca
      961 tagtttgcta aacagtcatc actgactgat gtcagtcaca ccatccgcca gttcaatcag
     1021 gcgaataaat tccgccctgt aaccctgtgg atcttcccct tttgcctgct gagcccactg
     1081 tttgatctgc tgccaggagg tattgttcag gtattcagaa ccgcgtaact tttgcccata
     1141 tgcagctact gctgcgcaaa aacgcatatc ttcagagggg gcgtttattg ttggccccag
     1201 cgggaattca actaactgac tttcttttcc ctgcgggtat ttccagcgaa tctttaacca
     1261 ggccagttct ttcgttttgt ccgatttcac tgatttgtta tccggggcat agcgtaactt
     1321 atcaattgat gctttttgcc cgttcagcgt taattcgaat aacaacgtta tatgtttgcc
     1381 tgcgccaata tcccccgcat caacgttgtc gttattaaaa tcttccgccc gaagttggcg
     1441 cttttcataa ccaatctgac ggtattccgt tacccacgcg gggttaaact caatttgcgc
     1501 tttgacatct tttgctacgg taatcaacgt ctgccgcatt tcactattca atactttctg
     1561 cgcttcagag agggtatcaa tgtagctgta gttgccgtta ccaacatcgg caattcgcac
     1621 catcattgcc tcgttgtaat tgctattccc cacgccaaac gtcgacagag taacaccaga
     1681 ctcccgctgt tttttgacca ttgattcaat cgattttgga tcgtcaatgc caacgttaaa
     1741 gtcaccgtca gtggctaata aaatgcgatt gatgccgccc ttaataaacc ccttcgccgc
     1801 ctgctgataa gccagttcca gcccggcacc gccattggta ctgccttcgg catccagcga
     1861 atcaattgcg gcattaattt ccgccttgtg actaccggag atagaaggca acgcaatacg
     1921 ggagtcgcca gcgtaggtca cgatggcaat gttatcctgc tcacgaagtt ctttgaccaa
     1981 taatttcaac gaagactgga taagtggcaa acgttcatca gaaatcattg aaccagaagt
     2041 gtcgataaga aagaccagat tagaagctgg taactcttca cttttatgat ctttcgccag
     2101 gatatcaatt ttcagcaatg ttagctgttc attccacggc gcaggagcca gttcgtagcg
     2161 catagcgaaa ggtattggct tagtggccgg aatagattgt ttgtctttga tatcccagtc
     2221 atacgggaaa taattgacta tctcctccac ccgcacagcg tctggcggag gtaacagccc
     2281 atgattgaga aaacggcgta cattcgcata actgccggtg tcgacgtcaa ggctaaacgt
     2341 cgccaacggg ttttgcgcta cctgcttaac cggattatca tcgaactgct tgtagcgagc
     2401 ggttcctgga tttgcgattt gggacgctgc tttttctctg gctgcatgtt gatattttgg
     2461 agccgcctgt aagcgcccct gcaaagcctg tttgtctgaa tattgttgcg cttcttgctg
     2521 ggctaaggct tttgctttcg tgtctgcagt ggctttatcg gcgacggatc tttgctcggc
     2581 ctcttttatt gcagcttgtt gcgcggcaag cacttgctgg tctgtggggg tactgggctg
     2641 ttgttgctga ctttccttat tctcagattc
//
LOCUS       DAZGLJ010000353         2649 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000353 DAZGLJ010000000
VERSION     DAZGLJ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.357"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1111
                     /locus_tag="ACHK49_004849"
     CDS             <1..1111
                     /locus_tag="ACHK49_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265319.1"
                     /translation="TSLTSILATLYKMLISEREVSEYECQLLYLTNPIDVMNILNKYI
                     YYFPNENSPFYTLKIDSALSWDAIDAIRDYSISDIYLYPEQKTINCVVEIENIVFGGY
                     IYTLNNGVTLQNIENSLKDSSCHYVLNGYTEFVNCLRQLTSGKTESVHRTINKLNYEK
                     LPFGFIIAAFAILKIAFKIKFSKNHVNIRALLNDINYFMTYQGESINLISLDHEYPES
                     CLQNDTNTYLLGRVIFLYNSMIYKFINCQEHETNNIHSAMINNLLQEVDIALGKINDI
                     IDSRNISAPHELANILTREKILTTREKKGNLISLFDGFTLFHCVGMITFLIHYLRTPE
                     EKVENIFMLYGADKNNKLRRRLIYDALGIIQSQQE"
     gene            complement(1298..2500)
                     /locus_tag="ACHK49_004850"
     CDS             complement(1298..2500)
                     /locus_tag="ACHK49_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9265320.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
BASE COUNT          829 a          587 c          471 g          762 t
ORIGIN      
        1 aacttcatta acaagcatcc tggcaacttt atataaaatg ctgataagcg aaagagaagt
       61 ttcagaatat gaatgccagt tattatattt aaccaaccca atagatgtaa tgaatatact
      121 gaacaaatac atatattact ttcctaatga gaactcacca ttttatacac tgaaaattga
      181 cagtgcatta tcgtgggatg ccattgacgc aatacgagac tatagtattt ctgatattta
      241 tctttatcct gaacaaaaaa caataaattg tgtcgttgag attgaaaaca ttgtctttgg
      301 cggttacatt tatacattga acaacggcgt cacattacaa aacatagaaa actctttaaa
      361 agattcttca tgccattatg tcttaaatgg ctatacagaa tttgttaact gtttgagaca
      421 acttacttca ggaaagactg aaagtgttca tcgcaccatc aataaactga actatgagaa
      481 attacctttt ggatttatca ttgccgcgtt tgctatacta aagatagcat ttaaaataaa
      541 attcagtaaa aatcatgtaa atatccgagc attattaaat gacatcaatt attttatgac
      601 ttatcagggc gagtccatta accttatttc actggatcac gaatacccag agtcctgtct
      661 tcaaaatgac acaaacacat atttattagg aagagtaata tttctgtata actcaatgat
      721 ttataagttc ataaactgtc aggaacatga aaccaataac attcactcag ctatgataaa
      781 taacctatta caggaagttg atatagccct tggtaaaata aatgacatta tagacagcag
      841 aaacatatca gccccccatg aactggcaaa tattcttacc cgcgaaaaaa tacttacaac
      901 acgggaaaaa aaaggaaacc tgataagcct gtttgatgga ttcactttat tccattgtgt
      961 tggaatgata acctttctta tccattatct cagaacacct gaagaaaaag ttgaaaatat
     1021 atttatgtta tatggtgcag ataaaaacaa taaactacgc agaagactga tttatgacgc
     1081 actaggaata attcagtctc agcaggagtg aagagttaaa caggcaaatt attttttata
     1141 aaaaggggat tgttaagtaa tcccctgtta atcaaaatac gctttcctga cgatctgata
     1201 attatcagct caatacattt gacataaaag cggtttctta attactactg ctacgatcac
     1261 atatcaaatc atgatcgtac aatatacatt ttcactatta tttattcaca atttgagaca
     1321 tcacataatc cgcccatgcc tgcatcatcg gtacacgctg ctccagtaga tcagtacgat
     1381 gatatgccgc ctcaacctta tttttcagcg tatgagcgag cgccctttcc gccagatccc
     1441 gcgaataccc ctgttcgcta caccagtccc tgaatgttga gcgaaaacca tgtgccgtgg
     1501 caactcgccc cggaatgtca ctgacggctt tctttttacg cagaaaactt gtcaacacca
     1561 tatcggaaag gatctgctgc tttctgggtg aagggaacac cagttcatca tgcaggccac
     1621 gtatattttc cagaatgtaa atagcctgcc gggataaagg aacacgatgc tgtatcctgg
     1681 ctttcattct ttctgcaggt atagtccata cccgcttatg aaaatcaatt tcagcccagc
     1741 gcattcccct ggcttcgccc gagcgagttg ctgtaagtat caccattaat aacagtgcgc
     1801 gggtaacatt ataaggttca tcggtataca cactggtcgc cacaaaaagc ggtaactgcc
     1861 tccagggcat tgcgggttgg tgttcatcac gtcctcttgt ctgctgagga agcaaatggt
     1921 caaccacatc aacaggattt gctacacaaa aaccgtgcgc ccatccccac tgcataacaa
     1981 catgaatgcg ctgtttaacc cggcttgccg tttctgacaa ggttaaccag actggacgca
     2041 gtgtttctgc cacgtccgca gccgtaatcg aatccagcgt ttttgctccc agttgaggaa
     2101 acgcgtaatt ctcaagcgtc gataaccact gccttacatg ctttggattt tcccatccag
     2161 gagacagttc tgcatgtaca cgcctggctg catcggcaaa tgttgggata gcgactttct
     2221 cagattcagc ctttttaatc tccagaggat catcacctgc agcaagttgc tctcgcatta
     2281 tccgggcagt acgtgcagct tcagcaatac tgacctctgg gtaagttccc aatccagcat
     2341 tacgtctttt ttgtgtcacc ggacttacat aacgaaaaac ccatttcccc cgccccttta
     2401 ctgaagaagg atgaagggtc agtccggtaa ttcccccatg gggcaatggt ttgtcatcag
     2461 gtttgatatg tcttgctttc gtatccgtca atactgccat acgctaattc ccgtcactct
     2521 ggtatgccat ccagtatgcc attagtctct cgcttcacta agaatacatt agataatatc
     2581 ggacaacaaa aagtgtaata cattgtttat aatgaaatta tataacaccc ctggattaag
     2641 tcagattta
//
LOCUS       DAZGLJ010000354         2628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000354 DAZGLJ010000000
VERSION     DAZGLJ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.358"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(95..763)
                     /locus_tag="ACHK49_004851"
     CDS             complement(95..763)
                     /locus_tag="ACHK49_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO9265321.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(1066..1659)
                     /gene="tehB"
                     /locus_tag="ACHK49_004852"
     CDS             complement(1066..1659)
                     /gene="tehB"
                     /locus_tag="ACHK49_004852"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO9265322.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(1656..>2628)
                     /gene="tehA"
                     /locus_tag="ACHK49_004853"
     CDS             complement(1656..>2628)
                     /gene="tehA"
                     /locus_tag="ACHK49_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO9265323.1"
                     /translation="NLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGLVILAMII
                     WGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFRPLAVCLF
                     SFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALGYTDAGLV
                     FLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSVNGGEGDT
                     LAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGSGSENGFF
                     HTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
BASE COUNT          733 a          660 c          582 g          653 t
ORIGIN      
        1 aggtggaaaa ccgcacctta tatccccgcc ctgaaggatg gggttttacg gcacaccgga
       61 taaaaagcgc catctaacga tggcgcgtct ggaactactt tttgttaacg tcaaacatgg
      121 tggcatcggt cgccatgtca tcaataactt gtttaatatt ttcgaaagcc attggcgtac
      181 tttcgttatt caggtcttta ccttcgccct gacgaacgac tttgataacc ggtttattag
      241 tcgctgcatc aatcagctca ccttcaaaat agaggcgagt atccatggta cggtggcctg
      301 tagccatttg cgtccccgca accactaatg caacaggaac cacttcatag aattgcagcc
      361 cttctttgct ggtatctaca ccggtaatgg ccccacggaa aatcagacta cgcggcccag
      421 cggtggtaac cagtggttta cgctgcgcta tcgcttcttt catttcggtg ttggtatagt
      481 tcaaaatttt atccagaact ttctgcccta cctgggtaga aggttttggc accggataat
      541 aagtgattgg gttccagacg atgctgtcat atttgctttg atcaaaactc gggtctaccc
      601 aacgtaaaac aggtttacct gttgctgagg ttgtttcttt taaatcagag taattgttta
      661 aaaaaccaga atatttatct ggctgggtga ttttagaggc acaaccagac agagccaaca
      721 agccgcataa agctgcaact ttcgcaaatg atgtggtacg catgaatagt ttcctgagat
      781 gattatatta ctttttaagt tatagcaaaa agcgccgctt cgtgttgtgg caaaaaaagt
      841 gttggtgaaa ataaattttt cagccccttt tcagacgaca tagtcaatgc cgaaaaaggg
      901 gctaagtcaa tcatcagtag ttaaatttca gtaagttaag ctgctaaaaa acttcgacta
      961 ctgtgattaa tttcacatcc cttcgataaa aaatatgctt accgaaaaga agaacagtaa
     1021 atcatctcag cgtggggaat tttcacgcgc cttattctta gcgggtcatt ttttacgtgc
     1081 cagcatcgtg gcgaaacgca gtttaatacg attaccgttg gcgtcggtgc ggtgcagctc
     1141 gccgacgtct tcattgtatt tcaccctctc ccagccttcg taatatcgac gtaattctcc
     1201 ctctttgaag gcaaacggga agccgacggt acatggataa tcagcggtat ccatcgccgc
     1261 cacaatcagg ttgtaaccac caggtttagt gcaacgttgc atattggcaa tcagccctgg
     1321 gatggtttta gcctcaagga acatcagtac cacagtcgaa agaataaaat cgtactgtct
     1381 atcaaatgtg aggttattca gatcaacgac tcgggtgtgt aaattatcca gattttcaat
     1441 ggatttaatg cgctctacat tggcgatact catggcattt ttatcccatg cgtcaacatc
     1501 ataaccattg gctgccaggt aaagactgtt acgaccattg ccacagccca gatccagcgt
     1561 tttacccggt ttaaccactt tcaccgcttc cagtacttca gagtgtgtgc gggttaattc
     1621 atatttatca gtaaaatagt tttcgtcacg aatgatcatt ctttgtcctc tgctttcatt
     1681 aaaacggcgc gctcggttct gactaacaat tttccctgca tcagaagcgc aaaagtacgg
     1741 atgagcagta ttgcaataat aaaattggta aagataaaca gcggtaccgc cagcgtatgg
     1801 aaaaatccat tttcgctgcc actccccaga tgcaaaccgg tggttgccag tgcagatacg
     1861 ccgaacgaga aactccagaa tgaagcatta aatggctggg agagatacca tggcatcaga
     1921 cgtagcataa acagcagttg cagcagtcca taaccaaaaa gcattttcgc cagcgtatca
     1981 ccctcgccgc cgttgacgct caaccaggca ctacaggcca ccagcgcagg agcgagctga
     2041 atgccgagtg atgtccgcag tgccgtgggt aattctcccg aactgcgcag acgctgcaag
     2101 atcacaggtt caaggcttag ccatgagaaa acgcctgcgc ctaaaaacac cagaccggcg
     2161 tcggtgtagc ccaacgcacc acaggccatt gcgctgataa agttgttggc aactgtcggc
     2221 agatacagtc caggcgtggt agcttcttca gggtgagatc cgcgccataa tcccgcagtt
     2281 tgccaggcgg cataagccaa ctgaaccacg acaccaaaac tgaacaggca caccgccagt
     2341 gggcgaaacc acggaacaaa accaatcgcc accagcatcg tcgttgccgg aaacaaactc
     2401 acaaaactgc tcagcactgg atggcgaact tccgccagca cgctatgcgg aaagcgtatg
     2461 agtcgggtaa taaatgcgct agttaataat ccccagatga tcatcgccag aatcaccagc
     2521 ccatccccta accagtggct gacctgccaa acctggctgg catagcgcca ggcaaatccc
     2581 atccctatcg tccccaacac aataccaaag tagcctgccg gcaaattg
//
LOCUS       DAZGLJ010000355         2605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000355 DAZGLJ010000000
VERSION     DAZGLJ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.359"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            45..998
                     /gene="ompT"
                     /locus_tag="ACHK49_004854"
     CDS             45..998
                     /gene="ompT"
                     /locus_tag="ACHK49_004854"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO9265324.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1185..>2605
                     /locus_tag="ACHK49_004855"
     CDS             1185..>2605
                     /locus_tag="ACHK49_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9265325.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKRENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFIAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHL"
BASE COUNT          824 a          559 c          540 g          682 t
ORIGIN      
        1 aaaatacata ttcaatcatt aaaacgattg aatggagaac ttttatgcgg gcgaaacttc
       61 tgggaatagt tctgacaacc cctattgcga tcagctcttt tgcttctacc gagactatat
      121 cgtttactcc tgacaacata aatgcggaca ttagtcttgg aactctgagc ggaaaaacaa
      181 aagagcgcgt ttatctagct gaagaaggag gccgaaaagt cagtcaactc gactggaaat
      241 tcaataacgc tgcaattatt aaaggtgcaa ttaattggga tttgatgccc cagatatcta
      301 ttggggctgc gggctggact actctcggca gccgaggtgg caatatggtc gatcaggact
      361 ggatggattc cagtaacccc ggaacctgga cagatgaaag taggcaccct gatacacaac
      421 tcaattatgc caacgaattt gatctgaata tcaaaggctg gctcctcaac gaacccaatt
      481 accgcctggg actcatggcc ggatatcagg aaagccgtta tagctttaca gccagaggtg
      541 gttcctatat ctacagttct gaggagggat tcagagatga tatcggctcc ttcccgaatg
      601 gagaaagagc aatcggctac aaacaacgtt ttaaaatgcc ctacattggc ttgactggaa
      661 gttatcgtta tgaagatttt gagctaggtg gcacatttaa atacagcggc tgggtggaag
      721 catctgataa cgatgagcac tatgacccag gaaaaagaat cacttatcgc agtaaagtca
      781 aagaccaaaa ttactattct gttgcagtca atgcaggtta ttacgtaaca cctaacgcaa
      841 aagtttatgt tgaaggcgca tggaatcggg ttacgaataa aaaaggtaat acttcacttt
      901 atgatcacaa tgataacact tcagactaca gcaaaaatgg tgcaggcata gaaaactata
      961 acttcatcac tactgctggt cttaagtaca ccttttaacg aagttaacca gattttctcc
     1021 ccggtggtat tgtataaccc cggggaattt gtttacagtt acaagtaggc aatatccact
     1081 aattttgtaa gcatttaacc tatgtcataa cgtgtaatct cttaccgcaa cacaagacct
     1141 caatatattc aaatcttatc tttattccga tattcagaga cacaatgcga aaaataatta
     1201 ctcatttcaa agttgtttta acgttacttc taccagtaac cgtatctgcc cagcagatac
     1261 agtggcaatc ctgtatggcc agtcaattca accactggtt tggtgaggaa aaaccgtctc
     1321 ctgacttact atgtggttat ttgtctgttc cattaaaata tacagacaca ggcggagatg
     1381 cttcttatga aaaaaaatca caagtcaaac tagcgttgac aaaattgccg gcaaaaagca
     1441 agcataaagg aagtatcctg ataataagtg gtggtcccgg gttaccaggc ataaatcctt
     1501 atattaactt tgactggcca gtcacaaatc ttcgtgagtc atgggatatt attggatttg
     1561 atcctcgagg cgtcggacag tccactccga caataaactg ccggcaatca gatacagaga
     1621 ctcaggaaaa cataaccgaa aagcaacaag tattaaataa aattaatgcc tgtatccata
     1681 ataccggagc cgaagtcatt cgccatatcg gctctaacga ggctgtatac gatattgatc
     1741 gtattaggca agccttgggg gataaacaac tgacagccgt ggcgtattcg tatggaactc
     1801 aaattgcagc cttatatgca gaacgttttc cctacaacgt aagatctatc gttcttgatg
     1861 gagtcgtcga tatcgatgac ctggaggaca acttcacatg gcaactcaaa caggcacaga
     1921 gttatcagga aacgtttgat cgctttgcat cctggtgtgc gcgtacaaaa agttgcccgc
     1981 tttcttcaga cagagataag gcaataactc agttccatga gctattatca aaattacatc
     2041 acaaaccttt attagacagt aagagagaaa atatatcttc agatgaactc atatcattaa
     2101 caacagacct tctgctatgg cgttcatcat ggccaaccct tgcaactgcc atacgccagt
     2161 tctctcaggg gattgtcagt aatgaaattg aaactgcgct cagtgctccg atagcctcag
     2221 aagagtcaag cgatgcttcg ggggtaatcc tctgtgtaga tcagggggat gagcaattaa
     2281 caccagaaga gcgaaaatcc cgaaaagacg ctcttgcgaa tgccttcccg gctattaact
     2341 ttgacaatgg acgttccgat tcacctgatt tttgtgaatt atggccaata catagcgacc
     2401 tgaacaaaac tcgcctgaaa aatactgttc tgccctctgg tttactgttt atagcacaca
     2461 aatacgaccc aacaacgccc tggattaatg cccgtaagat ggcagagaaa ttttccagcc
     2521 cgttactaac aataaatggt gatgggcata cattagctct caccggagtt aatttatgtg
     2581 tagataaagc agttgtacat cacct
//
LOCUS       DAZGLJ010000356         2602 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000356 DAZGLJ010000000
VERSION     DAZGLJ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.360"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            114..320
                     /locus_tag="ACHK49_004856"
     CDS             114..320
                     /locus_tag="ACHK49_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9265326.1"
                     /translation="MEAIRKIIFRQEVKKIIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEDDVLSWQKEREEEARGTAA"
     gene            335..811
                     /locus_tag="ACHK49_004857"
                     /pseudo
     CDS             335..811
                     /locus_tag="ACHK49_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            1004..1342
                     /locus_tag="ACHK49_004858"
                     /pseudo
     CDS             1004..1342
                     /locus_tag="ACHK49_004858"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            1373..1564
                     /locus_tag="ACHK49_004859"
     CDS             1373..1564
                     /locus_tag="ACHK49_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO9265327.1"
                     /translation="MATIPTLVHSQTAFLWRFIIFGASEHQIIHVTAWTEREARSRCP
                     SGCVAVFAARIRQEVCHVQ"
     gene            1554..1775
                     /locus_tag="ACHK49_004860"
     CDS             1554..1775
                     /locus_tag="ACHK49_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265328.1"
                     /translation="MCNKTTPDAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            1768..1989
                     /locus_tag="ACHK49_004861"
     CDS             1768..1989
                     /locus_tag="ACHK49_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265329.1"
                     /translation="MRDDRFNSLKQEFSGVPDDAADALSSISEIMRVAFFFLCTDEHR
                     DTGLNILDIAANYADFVTEAVLRKTTDGD"
     gene            1991..2224
                     /locus_tag="ACHK49_004862"
     CDS             1991..2224
                     /locus_tag="ACHK49_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265330.1"
                     /translation="MRDIYHETIDRAFSALAYAEGMYEILRIWLETLGDNERDKQKSR
                     IVTALITLLEPVINELQEIETLHDRYNEQHTGE"
     gene            2230..2529
                     /locus_tag="ACHK49_004863"
     CDS             2230..2529
                     /locus_tag="ACHK49_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9265331.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVAGRCVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
BASE COUNT          694 a          619 c          631 g          658 t
ORIGIN      
        1 caatataaaa aacggtgtat accgtttaaa aacggtggga actgttttta cccatatccg
       61 atgatttacc gtatttgtca ccggaataca ccggattcac aaggtaaatc acgatggaag
      121 caatcagaaa aattatcttt cgccaggaag taaaaaaaat tatccacatc aaggcagaca
      181 gcacgctgca aagcatgatc aacgccggag aatttccgca gggttttcgc gttggtttac
      241 gccgtcgcgg atggtatgag gatgatgtgt tgtcctggca gaaagaacgc gaagaggaag
      301 cacgcggaac ggctgcttaa cgggtatcac agatatgaca aacacgaaaa aagcggcccc
      361 gatatggagc cgcctttctg aacaattaac ccgctgcgcc ttatgtgtat gtgatcccaa
      421 acataagcac ggggatgata gccgctatca ggctggtggg caatgcaatc agtctggttc
      481 agttcgttgc catacctgca atgagcgctt ttccctgtac tctttaagga attgctcaag
      541 ggcaaaagca catggcgcga atctttctga ttcatgctct atctttctgc gccgtctttt
      601 ccgtgccggt gataatgttt tggtcaattc tttatcggtc attgtgttgt cctgcatagc
      661 aatgcgccgt aatacctcac accacggcgc tgatagtttt tatcctttgg gttctatgcc
      721 gcgttgctgt aattctttgc ggagaacgcg tttaatccaa gtggctaaag atgtgtcgcc
      781 atcggcctta gctgcttctt ctagctgcgc tctgaagtct tccggtaatc gcatttggta
      841 tggaggcgaa cgtttttcta atggtgttga cacggtaatt acccttcatt actataccta
      901 catggtaatt accattgtac ttgccattac acaaaaatca acgccccgca gtgctgggaa
      961 cacatacggg gcgtctaacc aacaacgtaa actaggagcc gttatggttg ccgtaaatca
     1021 tataccacac cttgtacaca cacaaacggc ctttgtgtgg cgttttctgg ccctgaatat
     1081 cggagaaaaa aaccagttga tcgccagtgt cgaaaatagc tatgattccc gcgcacctca
     1141 taaaacgggt gccgggattg gcgtcctggg caaaactgaa gcgcataaca cgcgccccgc
     1201 gtgttttttt gtgccgcata gtcacacctt atcaatggtg ggctgtacgg gggcggagca
     1261 atccgcgccg gtttcttcag tgtccggtta cgccaaccct gtacagtccg ccaccagcga
     1321 aattggcgtt tccggtggcg gttatcttaa ccacactgag gaggctgcca acatggctac
     1381 tatccctacc cttgtacatt ctcaaaccgc ctttctctgg cgctttatca tctttggcgc
     1441 gtcagaacat caaatcatcc acgtaaccgc ctggacggaa cgcgaagcgc gtagccgttg
     1501 cccgtccggt tgtgttgctg tattcgccgc ccgtattcgt caggaggtgt gccatgtgca
     1561 ataaaaccac accggacgca gcagccgccg cgctcactac gctgatgcac gcgcttatcg
     1621 atattgaatg cactgcagag cttgcgcagg gagaagaaca gaaagaccgg acacagttcg
     1681 ccctggaatg tatccgatac atcgcaacgc ggtcgctgaa tgacgctaaa aatattcttg
     1741 ttgctgattg tgaaaatggg gggggttatg cgtgatgatc gttttaattc cctgaaacaa
     1801 gaattttccg gcgttcctga tgatgcggct gatgcgcttt cgtcaatatc tgaaattatg
     1861 cgggtggctt ttttctttct ttgcactgat gagcacagag atacagggct aaatattctt
     1921 gatattgccg ctaactatgc tgatttcgtg acagaagctg ttttaagaaa aacaacggac
     1981 ggggattaat atgcgtgata tttaccacga aacaatagac cgcgcattta gtgcccttgc
     2041 ttacgctgaa ggtatgtacg aaatattgcg catatggctt gaaacacttg gcgacaatga
     2101 acgcgacaaa caaaaatcaa gaattgtcac ggcattaata acgcttcttg agcctgtaat
     2161 aaatgagttg caggaaatag agacattgca cgacagatat aacgaacagc acaccggaga
     2221 ataaaaataa tgaaacttaa atattctggc ttaactgcca gtggcaacac tcaccctaaa
     2281 tttacgcgcg gtgatattta ccgcgaccag tacggcggca cggtaatgat taagggcgtg
     2341 gcgggacggt gcgtaactta ccgccgtgaa ggttacgaat atgattgcgt gatgcctgtt
     2401 tatcagttcc ggcgtgattt ttctctggta cagaccgcgc cgcataacgt gcccaccagc
     2461 aacgccaggg cacgggcaaa catccagaag ctgaaaacca tgattaacgg attcaggggc
     2521 aagaaatgaa aagcgcaccg aacttaaaaa aacagcctta cgacaagatg accgaagtca
     2581 ttatttttgc gggtagtgat gc
//
LOCUS       DAZGLJ010000357         2602 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000357 DAZGLJ010000000
VERSION     DAZGLJ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.361"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..700
                     /gene="yciT"
                     /locus_tag="ACHK49_004864"
     CDS             <1..700
                     /gene="yciT"
                     /locus_tag="ACHK49_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO9265332.1"
                     /translation="VSVTDLAKATGVSEVTIRQDLNTLEKLSYLRRAHGFAVSLDSDD
                     VETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLARTLGEQKKNVTIITVSS
                     YIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHFSKAFIGIDGWQPETGFT
                     GRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVERFNRVITDSKIRASDLM
                     HLEHSKLTVHVVDI"
     gene            969..1187
                     /gene="osmB"
                     /locus_tag="ACHK49_004865"
     CDS             969..1187
                     /gene="osmB"
                     /locus_tag="ACHK49_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO9265333.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(1313..1639)
                     /gene="yciH"
                     /locus_tag="ACHK49_004866"
     CDS             complement(1313..1639)
                     /gene="yciH"
                     /locus_tag="ACHK49_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO9265334.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(1639..2376)
                     /gene="pyrF"
                     /locus_tag="ACHK49_004867"
     CDS             complement(1639..2376)
                     /gene="pyrF"
                     /locus_tag="ACHK49_004867"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO9265335.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
BASE COUNT          694 a          648 c          604 g          656 t
ORIGIN      
        1 ggttagcgta accgatctgg caaaagccac tggagtttct gaagttacca ttcgccagga
       61 tctcaacacc ctcgaaaaac tgagttacct ccgccgtgca catggctttg cagtttcgct
      121 tgatagtgat gacgtcgaaa cccgtatgat gagcaactat acgctgaagc gtgaactcgc
      181 cgagtttgcc gcgtctctgg ttcaaccggg cgaaaccatc tttatcgaaa atggtagcag
      241 taatgccctg ctcgcccgga ctctgggcga acagaagaaa aatgtcacta tcatcacagt
      301 cagcagctac attgcgcatt tgctaaaaga cgcgccttgt gaagtcattt tgctcggtgg
      361 cgtgtaccag aaaaaaagcg aaagtatggt tggccctttg acacgccagt gcatccagca
      421 ggtacatttc agcaaagcat ttattggtat tgatggctgg caacctgaaa ctggattcac
      481 cggtcgcgat atgatgcgta ccgatgtggt caatgccgtg ctggaaaaag agtgcgaagc
      541 gatagtcctg actgacagct cgaaatttgg tgctgtacat tcatactcca tcggtcccgt
      601 tgagcgattc aatcgcgtga ttaccgattc gaaaatacgc gccagcgatc tgatgcatct
      661 tgagcacagt aaactcaccg ttcacgtcgt tgacatttaa ccatttccct tctctacgga
      721 tgatttgcag tttggcaaat catccgctct aagatgattc ctggttgata attaagacta
      781 tttacctgtt attaacacgc ttaagatata aaattattat cagcgatata acaggaagtc
      841 attatcacct gcgtgatata accctgcgcg cgagcagatt tcacggaata atttcaccag
      901 gcttattctt agctattata gttatagaga gcttacttcc gtgaatcata aattcaggag
      961 agagtattat gtttgtaacg agcaaaaaaa tgaccgcggc tgttctggca attactttgg
     1021 caatgtctct gagtgcctgt tctaactggt ctaaacggga ccgcaacacc gcaatcggcg
     1081 cgggtgcagg ggcattaggc ggtgcagtac tgaccgatgg cagtacgttg ggtacattag
     1141 gtggtgcagc tgtcggtggt gttattggtc accaggtcgg taaataatcg taaataaatg
     1201 tcgattatcg actcgcaatt ataatacgct tgattctgtt cagtacgaaa taactgaata
     1261 atacacacag taaaatattc gaaagccacg gatatatccg tggcttttta tgttaaccgc
     1321 ctgcgagttt taccttcatc cctttcgctt caagtagtga ttttaataaa tcacgcttat
     1381 cgccctggat ttcaataact ccatctttaa ctgctccgcc gcagccgcat tttttcttca
     1441 gttccgctgc aagttttgtc agttcggcat catcgagatc gacaccggta atcaggcaaa
     1501 cgcccttacc tttacgtcca ctggtctgac gctgaatacg caccacaccg tcgcctttag
     1561 ggcgtacagg ggccgctttg ggctcatcaa tacgtccggt ttccgttgag tagaccagac
     1621 ggctgttgga atcactcatc atgcactccg ctgtaaagag gcgttgatcg ctttcagcgt
     1681 ctgcgctgga tctaccgatt gcgttaccgg gcgaccaatc accatataat caacaccagc
     1741 cgacaacgcc tgttctggcg tcataatgcg gcgctggtca ccagcttcac tcccctgcgg
     1801 acgaatgccc ggcgtaacca gtttgaactc ctgaccgaat acctgtttaa agcgcacagc
     1861 ttcctgagca gaacacacca caccatcaag gccacatttt tgcgtcagtg ccgccagacg
     1921 ttctgcataa tctgcaggtg acagtgtcat gccaagatcg accaggtcgc tggcttccat
     1981 gctggtcaac actgtcacag caatcaaaag cggtgcatct ttgccaaacg gaaccagtgc
     2041 ctcacgcgct gcggtcatca tacgcgcccc accagaggca tgaacattca ccatccacac
     2101 gcctaagtca gctgcagcag cgacagcgtg cgcagcagtg ttaggaatat cgtggaattt
     2161 caggtcaaga aagatatcaa aaccacgctg ttgaagttcg cgcacaaact gtggcccaaa
     2221 caatgtaaac atctctttgc cgaccttcag acgacaatcg cgtgggtcga tcttgtcgac
     2281 aaaggacagc gcgtcatcac gattatgata atcaagggca acaaccacag gagaattcgt
     2341 aacagcgcgg gaagaagatg aagcagttaa cgtcatgacc agaccttctt gatgatgggc
     2401 accggaagtg gcgcgaaaca ggtaaacggc gagcattcta cccgtggaaa taaaaaatta
     2461 acagttgcga tttttcccct ggcaggcttt ttctgcgccg tcgttgccgg agcacaatgg
     2521 aacatcaata actagtatgt tgtaactaaa gtaggatttt ttaaaaatta caggccatca
     2581 agaccgcgaa tcggtttaat gg
//
LOCUS       DAZGLJ010000358         2598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000358 DAZGLJ010000000
VERSION     DAZGLJ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.362"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            427..570
                     /locus_tag="ACHK49_004868"
     CDS             427..570
                     /locus_tag="ACHK49_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO9265336.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            583..792
                     /gene="cspE"
                     /locus_tag="ACHK49_004869"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             583..792
                     /gene="cspE"
                     /locus_tag="ACHK49_004869"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO9265337.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            958..1767
                     /gene="rlmA"
                     /locus_tag="ACHK49_004870"
     CDS             958..1767
                     /gene="rlmA"
                     /locus_tag="ACHK49_004870"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO9265338.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1764..2330)
                     /gene="mntP"
                     /locus_tag="ACHK49_004871"
     CDS             complement(1764..2330)
                     /gene="mntP"
                     /locus_tag="ACHK49_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO9265339.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          689 a          611 c          620 g          678 t
ORIGIN      
        1 gattttttgc tcattcatca atttttctta ttttaaattt tcaatccctt tggggattga
       61 cttctcttta gggtaattga tagccgttaa ctgactgttt tatgagaaaa agtgatataa
      121 ctttttattc attgcatagc aaaaaatgtg atattgcacg cactatgtaa taacttctcc
      181 cactggcctg gaacaactga acttattgaa ctatgttaga aaatacgcca gtttaagtat
      241 ctgcctgaac tggcaaggtt aagcacaatg atatatcggc gcgtattccg ttgcataagt
      301 gtgcaaaaaa agtggaagac gtatcgagat ttgtgcgtct gatcgagaca tgtttaaaaa
      361 tggcttgcca taattaacgt tgtatgtgat aacagatttc gggttaaacg aggtacagtt
      421 ctgtttatgt gtggcatttt cagtaaagaa gtcctgagta aacacgttga cgttgaatac
      481 cgcttctctg ccgagcctta tattggtgcc tcatgcagta atgtgtcagt tttatctatg
      541 ttatgcctgc gggcgaagaa aacaatctaa ggaatttttc aaatggcaaa gattaaaggt
      601 caggttaagt ggttcaacga gtctaaaggt tttggcttca ttactccggc tgatggcagc
      661 aaagatgttt tcgtacactt ctccgctatc cagggtaatg gcttcaaaac tctggctgaa
      721 ggtcagaacg ttgagttcga aattcaggac ggccagaaag gtccggcagc tgttaacgta
      781 acagctatct gatcgaatcc actgatctga agtgtgaata cgcttcaatc tcgctataaa
      841 gcctcgtcga atgcgaggct ttttactatg ctttatcttc gctcctggcg ttcggatatt
      901 tgcccgccgc gtgattcgcg ttacacttgc ggcctttagt atcctgccgg agttgtcatg
      961 tcgttttcct gtccactttg ccatcagcct ctttcgcgtg aaaaaaacag ctatatctgt
     1021 ccccagcgac atcagtttga tatggcgaaa gaagggtatg tcaatctgct gcccgttcag
     1081 cataaacggt ctcgtgatcc gggcgacagc gcggaaatga tacaagcacg ccgcgcattc
     1141 ttagatgccg gacattatca gccgctgcgt gatgcaattg tcgcccaact gcgggaacgg
     1201 cttgatgaaa aggccacggc ggtgctggat attggctgtg gtgaagggta ttacacacac
     1261 gcatttgccg atgtgttgcc cgaaatcacc acgtttggcc tggatgtctc gaaggtagcg
     1321 ataaaagcgg cggcgaaacg ctatccgcag gtcactcttt gtgtcgcttc cagccaccgt
     1381 ttgccgtttt ccgataccag tatggacgcc ataatacgta tttacgcgcc gtgtaaagca
     1441 gaagaattag cacgagtagt gaagcccggc ggctgggtca ttactgccac gccgggaccg
     1501 cgacatttga tggagctgaa ggggctgatt tacaatgaag tacatcttca tgcacctcat
     1561 gcagaacaac tggaaggttt tacattacag cagagtgatg agttgtgtta tccgatgcgt
     1621 cttcgcggtg atgaagccgt cgcattattg cagatgacgc cgtttgcctg gcgtgcgaag
     1681 ccagaagtct ggcaaacact ggcagcaaaa gaagtgttcg actgccagac ggactttaat
     1741 attcacctct ggcagcgttc ttattaaccg tggaagtgcg tccagaggat ctggacgccg
     1801 atgccgatca gcaccagccc gccgagaatt tccgcttttt tcccaataat tgagccgata
     1861 aagcgaccaa ccatcatccc taatgtcgac ataatcaagg ttgcacaacc aatggccaat
     1921 gcggtcgcga taatgttgac ctgcaggaaa gcaagaccaa cacccacagc catggcatcc
     1981 aggctggtgg caatcgcggt ggttaccagt agccagaaac cgtgccgacg gcgcggctct
     2041 tcatcttcat catctgcgcc acgaaaaccc tcaataatca ttcgcccgcc gaggaatatc
     2101 agcagcacaa acgcaatcca gtggttccat tcaaggacaa accggctggc taacatgccc
     2161 attccccagc cgatcagcgg cgtcagggtt tcgacggcac caaaaataag gccggttcgc
     2221 aatgcttcag aaaatttcgg tttatggagg gtggcacctt taccgattga tgcagcaaat
     2281 gcatccatcg acataccaaa cgcaagaaga acagtagcag tgatattcat gacaatgtcc
     2341 tgaccggggt atccataaaa cacatcatcg cccccagcaa acatgcatac atgaacaaca
     2401 agccgcgatg gttttcgcgt tcatgcaggc atagcgattg atgtgcatat ggtctcgcct
     2461 gactgaaagt attcagtccg tacgtgccac gttttgcaac gagtatgttg acacgtacat
     2521 ttccggaatc tggaaatcgg ctactcccca aatgagggcg caaccttaac atattttgag
     2581 aatataaaac aactgttg
//
LOCUS       DAZGLJ010000359         2545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000359 DAZGLJ010000000
VERSION     DAZGLJ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.365"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            235..600
                     /locus_tag="ACHK49_004872"
     CDS             235..600
                     /locus_tag="ACHK49_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO9265340.1"
                     /translation="MSDMTISLPKEVNGHVICSTRIRHILAIRKSILMQIYMLFRQQA
                     GISALRQELEVKTPYRAMNHPVIGVVTKADLASMEHISLVKCWLREAGAHNVLVTSAV
                     NNNGVTELFALLHTEEGCC"
     gene            complement(701..1030)
                     /gene="tmaR"
                     /locus_tag="ACHK49_004873"
     CDS             complement(701..1030)
                     /gene="tmaR"
                     /locus_tag="ACHK49_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO9265341.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1202..2260)
                     /gene="yeeA"
                     /locus_tag="ACHK49_004874"
     CDS             complement(1202..2260)
                     /gene="yeeA"
                     /locus_tag="ACHK49_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO9265342.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLIDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSSLICRALRK"
     gene            complement(2458..>2545)
                     /locus_tag="ACHK49_004875"
     CDS             complement(2458..>2545)
                     /locus_tag="ACHK49_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase inhibitor"
                     /protein_id="HFO9265343.1"
                     /translation="CFEVYLNNGAEDGYWDIEMYVAVQPKHH"
BASE COUNT          705 a          614 c          589 g          637 t
ORIGIN      
        1 cctactgatt gctaaggata aacaagtatt aaaggccaga ctgataagac cgcgactaag
       61 tgaacctgta tctgacatgg catagcttcg ctaatgtgtc atcatgatta ggactaaatc
      121 tgtgaattcc tttaggaagc acggacacag gccaatctga gtccatatgt atgattggaa
      181 tcttacacag atgacttttt gacacgggga cttggcaaac aggggcgacc atgtatgtca
      241 gatatgacaa tctcacttcc gaaagaagtc aacgggcacg ttatctgttc aaccagaatc
      301 cgacacattc tggctatacg aaagagcata cttatgcaaa tctatatgct tttccggcaa
      361 caagcaggaa tctcagcgct gaggcaggaa ttagaggtaa aaacgcccta ccgggccatg
      421 aatcacccgg tcattggagt agtaaccaaa gcagatttag ccagcatgga acacatatct
      481 ttggtgaaat gttggttacg ggaagctgga gcgcacaacg tgttagtaac cagtgcggtt
      541 aataacaatg gagttacaga actttttgcc ttgctgcata cagaagaagg ctgttgctaa
      601 tttcgatagg ttctcagatg agaatgatga cggtgataca cagataagaa tcaatgagca
      661 atgagggttg ccgggcaacc ctcattgaat aaaacaggaa ttacttcgct tcgccgtttt
      721 tcatttcgcc catagctttc agctttttgg agatatcgcg gcgttctttg gagagctcgg
      781 catttttgat gatgtaatcg tcaacgcgat cttcatagtc acctttcatg ctggcgatga
      841 tgccctggat tgcttcaacg ctcatcccgg gcttgatgta atcgctcagg ttgtccagca
      901 acaggacgcg cttctggttg tcacggatct ttttctcaac gtcctgaatt tcacgttgca
      961 gtttgttctt acgacggaac agacgaacaa attccagtac gtcctggaat gaaggcttgg
     1021 tagtttccat ttttataccc ctgataatgt gagagtcgga ttcgtttaag caaccgctat
     1081 tcgttagggc caacattact catagctgcc gcagatgaca atgcttttat cccttttcac
     1141 tatcataccc tttatccttg ctgaatcgaa gcagcagcaa gatgattctg aagttcagga
     1201 attatttgcg caaggcccgg caaatcagac tcgatagcaa ctcaagctga tgtgccgttt
     1261 ccatgttcag ccacacataa ccatagattg gtgtttccac aaccttcagg tcatggtggt
     1321 tattcagcaa ctgacgcagc tcttccacag catcgatcaa tttttccgta ttggcaaaaa
     1381 ccggctgcgg attaccttcg tacagcgcat gaacaaggct cagcagtatt tgctgcatca
     1441 tgtgctgggt atcacgaagt ttttgcgcgt tcaataacac gaaatggctg gggcgcgtgg
     1501 cccagtatgc attgatttgc aactccagca tacaaaccag attgcggtta atggtctgga
     1561 taccttcata tatcgatttt ggaatacgag tttctttgct ggcgggcgca atcagcccac
     1621 gcattttcac ggcatcggtc aggagttttt gtagatggct ttccagacgt gggcgttcga
     1681 gtaagttcgg tgagaatgca gattgataaa cccgattata ctcggtcaga cttttcgcca
     1741 gttgaatgcg ccagtggatg aacgcccgtt gtggccagat accggtaaac aacattgcca
     1801 gtaaagagcc gaggatcaca tcgccgcttc gccataacgc cgtatcaatt tcacctgtcg
     1861 gggaacccac aacaattgcc agcgtcaccc caatcaataa tccttgatac ggtttcttgc
     1921 ccagcgccag ccaaccgcaa agaaacatag ccgccgcgca ccagactaac atcagcggta
     1981 acgaaattaa ctccagttgc agagcgataa gacctaaaat cgaacccaac accgtaccgc
     2041 caatacgttc gaaggcgcga gggacaacgt taccccagaa cgagattggc cccataatca
     2101 ccaccatggt gaccagcggc caggtgcttt ccgggatagt aaacaggcgg ataatgagaa
     2161 aagtgagcag gaatgccagc gcgacccgag taccatgcac aatgcggtaa tggcggtata
     2221 cccggatttc aaacgggctt aatgacttat cggcacgcac gcagaaactc cgtaatgaag
     2281 attaaaagtg ctcataactc atctgtcggc cagaaggccc gcctctgaat tatccgacgg
     2341 acaaaccggg aacagtgtaa tttcccggca ggagcgaaaa aattgaaagg cgcatcttac
     2401 tcttttcgct ttcaaaaaaa gagtggtcat cgcgttaaca caccgccctg agatgaatta
     2461 gtgatgtttt ggctgcaccg caacatacat ttcgatatcc cagtacccat cttccgcgcc
     2521 attgttcaaa taaacctcga agcag
//
LOCUS       DAZGLJ010000360         2543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000360 DAZGLJ010000000
VERSION     DAZGLJ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.366"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..487)
                     /gene="curA"
                     /locus_tag="ACHK49_004876"
     CDS             complement(<1..487)
                     /gene="curA"
                     /locus_tag="ACHK49_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO9265344.1"
                     /translation="MRQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PV"
     gene            668..1186
                     /gene="mddA"
                     /locus_tag="ACHK49_004877"
     CDS             668..1186
                     /gene="mddA"
                     /locus_tag="ACHK49_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO9265345.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1183..1632
                     /gene="ydcZ"
                     /locus_tag="ACHK49_004878"
     CDS             1183..1632
                     /gene="ydcZ"
                     /locus_tag="ACHK49_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO9265346.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1633..1866)
                     /gene="ydcY"
                     /locus_tag="ACHK49_004879"
     CDS             complement(1633..1866)
                     /gene="ydcY"
                     /locus_tag="ACHK49_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO9265347.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1952..2125)
                     /gene="ortT"
                     /locus_tag="ACHK49_004880"
     CDS             complement(1952..2125)
                     /gene="ortT"
                     /locus_tag="ACHK49_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO9265348.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2320..2415
                     /gene="yncL"
                     /locus_tag="ACHK49_004881"
     CDS             2320..2415
                     /gene="yncL"
                     /locus_tag="ACHK49_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO9265349.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          609 a          633 c          619 g          682 t
ORIGIN      
        1 ccacaggacc tgtcgccgca gctaccacca acgtttcgcc ctctttaggc tgaccgatat
       61 ccagcagccc catataagcg gtaaagcctg gcatccccag cacacccagc gaccacgatg
      121 gattttgcgg atgatcgcca agtttcacca gatcatcacc actggatatg tcatagtcct
      181 gccatccact gtagcccagc acccagtcgc caggctgata atcaggatga ttcgactcca
      241 caacacggct caccgtgccg ccgaccatca cgccgccaat atcaacaggc ggtgaataag
      301 atggctcatc gctcatacgt ccacgcatat acgggtccag ggacaaataa actgtgcgca
      361 gtaacacctg tccttcaccc ggtgtggcga catcatcttc ttcaagacgg aaattctccg
      421 gaaccggtgc gccatgtgga cgcgaggcca gaacccaacg tcgattacgc tgcttttgtt
      481 gcctcataat gctctcctgt gcttttggca tgaatccatc aatagtagca ggctcacggg
      541 attggttttg tttgcagccg tcacatcccc tctgcaacac gcaataattt catcgaggaa
      601 tccgttgctt cgggctgttt tcgatagtat tgataacatc atcatcatta ttttatcgag
      661 gccagacatg tccatccgtt ttgcccgcaa agccgattgt gctgccattg cggaaattta
      721 taaccacgcc gtgttgtata cggcggctat ctggaatgac caaacggttg atgcagataa
      781 ccgcattgcc tggtttgaag cgcggaccat agcaggttat ccggtgctgg tgagtgaaga
      841 agatggcgta gtgacgggat atgcctcgtt tggcgactgg cgtagtttcg atggttttcg
      901 ccataccgtg gaacattcgg tttatgtcca tcccgatcat cagggcaaag gtctggggcg
      961 taaattgtta agccgattga ttgatgaagc gcgggattgc gggaagcatg tcatggtcgc
     1021 cgggatcgaa tcgcaaaatc aggcctcgct gcatctgcac cagtcgctgg gatttgtcgt
     1081 caccgcacaa atgccgcagg tgggcactaa atttgggcgc tggctggatc tgacatttat
     1141 gcagttgcaa ctcgacgaac gcactgaacc ggacgcgatt ggatgaatca gtcgctcacc
     1201 cttgcttttt tgattgccgc cggaattggt ctggtagtcc agaacacgct gatggtacgc
     1261 atcacccaga cctcctctac cattctcatc gccatgttgc tgaactcact ggtggggatt
     1321 gtgctctttg tttctatttt atggtttaaa caaggcatgg cggggtttgg cgaactggtg
     1381 tccagcgtgc gctggtggac acttataccc ggcttgctgg gatcgttttt tgtcttcgcc
     1441 agtatcagcg gatatcagaa tgtgggagcg gcgacaacta ttgcagtact ggtcgccagc
     1501 cagctaattg gcggtctgat gctggatata ttccgcagtc atggcgtacc actacgggcg
     1561 ttgttcggcc caatttgtgg cgcaatttta cttgtggttg gcgcctggtt ggtagccaga
     1621 cgctcatttt aatcagagga tggtgccgcc tttggttaac tgttcgtggc gcgcttccat
     1681 atcttcttta tgcttgcgac cgtgatgagc aatagcggtg cgcaaacgct gctgctgggt
     1741 gtagcgatct tcccgactta actccgcgtc atcgctcagg gcgattaata attcgttcat
     1801 atgggcaatg acgctctctt caatcgcggc atccacgcgc gcgatgactt catccagatg
     1861 cgacatactt tctccttaac cacttgtgaa atttatcgat tcatccaaat gatataagtc
     1921 tgcgtggtgc cgtcaagcca gtgagtaaga tttaaaacag tgaaaacaac agcgccaccg
     1981 ggaaactcat cggccatgtt gcccccacca gaaaggcgct taagaaacgg atgcgtttct
     2041 tatcgtgaga aagaaaccag gtgataagaa atgcgattgc tgccataacc gcataaaaaa
     2101 caagcatatg ttgatagaga gacatgtacg ggcgtccagc cagatgaata aaacgcgcgc
     2161 aaatatgcgt cattttttga cgtgcatcaa gttttttcac cagtttgaga aaaaatcatc
     2221 aaacagcaac ttgcccttat ccatcttcag aatgagtatt attgcggatt ttcttaaccc
     2281 gtactataca cataggggca atgataaaag gtggcaaaaa tgaatgtttc cagtagaact
     2341 gtagtactga taaatgtctt tgctgctgtt ggtttgttta ctcttatctc tatgagattt
     2401 ggctggttta tttgatgtag tgattttgca agcagcacgt actgtcagag aaatcaactc
     2461 tctgacagta cgcaagatct tctcatactc tccaggttta caacattatc cttaatattt
     2521 acttcttttt gctggcatct aca
//
LOCUS       DAZGLJ010000361         2536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000361 DAZGLJ010000000
VERSION     DAZGLJ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.367"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..239)
                     /gene="escO"
                     /locus_tag="ACHK49_004882"
     CDS             complement(<1..239)
                     /gene="escO"
                     /locus_tag="ACHK49_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase activity
                     positive regulator EscO"
                     /protein_id="HFO9265350.1"
                     /translation="MAKINSQIKEVDGKLDDCEQAIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLSKEKRSLLDSQ"
     gene            complement(299..1639)
                     /gene="escN"
                     /locus_tag="ACHK49_004883"
     CDS             complement(299..1639)
                     /gene="escN"
                     /locus_tag="ACHK49_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFO9265351.1"
                     /translation="MILEHDSVLERYPRIQKVLNSTVPTLSLNSSTRYEGKITNIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHISGMYCGQWLSYQGEEF
                     KIRVGDELLGRLVDGIGRPMGSNITAPYLPFERSLYAEPPDPLLRQVIDQPFTLGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEFFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVKSEHLRAAAECKKLIATYKN
                     IELLIRIGEYTMGQDPEADKAIKNRKAIQSFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1623..>2536)
                     /gene="escV"
                     /locus_tag="ACHK49_004884"
     CDS             complement(1623..>2536)
                     /gene="escV"
                     /locus_tag="ACHK49_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFO9265352.1"
                     /translation="MRWNFFEESGIPLPKIIVNPVKNNDSAIEFLLYQESIYKDTLTD
                     DTVYFEAGHAEISFEFVQEKLSANSIVYKTNEANQQLAHVSGMDVYAKTNDKITFLLK
                     KLVLSNAKEFIGVQETRYLMDIMERKYNELVKELQRQLGLSKIVDILQRLVEENISIR
                     DLRTIFETLIFWSTKEKDVVILCEYVRIALRRHILGRYSVRGTLLNVWLIGSDIENEL
                     RESIRQTSSGSYLNISPERSEQIIGFLKNIVNPTGNGVILTALDIRRYVKKMIEGSFP
                     SVPVLSFQEVGNNIELKVLGTVNDFRA"
BASE COUNT          725 a          602 c          469 g          740 t
ORIGIN      
        1 ttttgtgaat cgagcaatga acgcttttct ttagaaagtg aggatttttt ctcatataat
       61 cgctgctttt gctcatcaaa tgcattgttt ttaatctggt atttatacaa cgacacttta
      121 tccagattca ccagcgatgc acaatacgcc tgcttagagg caatcgactc tttaatggct
      181 tgctcgcagt catcgagttt accatccacc tcttttatct gagagttaat cttagccatt
      241 gattctctca atcgattcgc tctgctttta cgaatagata aaattctgtc caacatattc
      301 aggcaaccac tttgaatagg ctttcaatcg ttttttcgta actactgata tctttggtgc
      361 tttgttgtat aaagctctga attgctttcc tgttttttat tgccttatcc gcttcaggat
      421 cttgccccat cgtgtactca ccaatacgaa taagcagttc aatattctta taggttgcaa
      481 taagcttttt gcattcagcc gctgcacgta aatgttccga tttaacaacg ttatgcataa
      541 cacgactggc gcttaaacca atatcaattg cagggaaatg gttttcctct gcaagttctc
      601 gggtaagcac aatatgtcca tcaagaatag agcgaacttc atcaccgatg ggatcattca
      661 cattatcgct ttctaacagc accgtataaa tcgcagttat tgaacctttt ggcgcaggtc
      721 ccgcacgctc taataatttg ggtaacgagg aaaaaacact cggagggaat ccccctctta
      781 catcaggctc tcccgatgca agcccaacat cacgcgcggc tcgggcataa cgagttacgg
      841 aatccatcat taataataca tttttgccct gatcccggaa gaactctgca atggttgtcg
      901 ccgtaaatgc ggccttcatt ctttccagcg cgggacggtc tgacgttgtg acgaccagta
      961 cacatttaga aagcgtggat tgaggtaaaa gcgcgaggaa ttcatttact tcgcgaccac
     1021 gctcgccgat aagggcaagg acaataatat ccgcggatgc gccattacaa atcattccca
     1081 aaagcgtact tttaccaacg ccggaccccg cgaaaatacc gatgcgttgc ccaataccgc
     1141 aagtaagtaa accatctatt gccctgacgc caagcgtaaa tggctgatca ataacctgcc
     1201 ttaataaagg atcggggggt tcagcataca aactgcgttc aaacggaaga taaggtgcag
     1261 taatattgct ccccattggt ctgcctatac catcaaccag tcgcccaagg agttcgtccc
     1321 caacacgaat cttgaactct tccccctgat aacttagcca ctggccacaa tacatgccgg
     1381 aaatatgctc aaaaggcagc aggaaaacct catcttcatc aatagcgatc acctcagcta
     1441 aacgttgact gggttctatc ttgtaaaagg caccaatacg cgcttttggt aaacgcgcct
     1501 tgataatcgt cccgccgata ttggtaatct tgccttcata tctggtagac gaatttaatg
     1561 ataatgtcgg cacagtgcta ttgagcactt tctggatacg tgggtatctt tccaatacag
     1621 aatcatgctc taaaatcatt taccgttcct aatactttaa gttcgatatt attcccaact
     1681 tcctgaaaag agagcacggg gactgacggg aacgaacctt caatcatttt cttcacatag
     1741 cgcctgatat ctaaagcggt cagaatgacg ccattccccg ttggattcac aatatttttt
     1801 aagaagccaa taatctgctc acttcgctcc ggtgagatat tcaaatacga acctgatgac
     1861 gtttgtctga tcgactctcg taactcattt tcaatatcag agccaataag ccagacattc
     1921 agaagtgtac cgcgaacgct atagcgacct aaaatatgcc gacggagggc gatacgaacg
     1981 tattcgcaca agataaccac atctttttct tttgtggacc aaaaaataag cgtttcaaaa
     2041 atagttcgta gatctctaat tgagatattt tcctctacga gacgctgaag aatatccaca
     2101 atcttgctca gaccaagctg acgctgaagc tctttcacaa gttcgttata tttcctctcc
     2161 atgatgtcca tcagataacg cgtttcttgt acgccgatga actctttggc attagacaat
     2221 acaagtttct ttagcaaaaa cgttatcttg tcatttgttt ttgcataaac atccatacct
     2281 gagacgtgag ctagttgttg gttagcctca ttcgttttat acacgataga gttagccgaa
     2341 agcttttcct ggacaaattc aaatgatatc tctgcatgcc cagcctcaaa ataaacagta
     2401 tcatctgtaa gagtatcttt gtatattgac tcttgataga gcaaaaattc tatagcgcta
     2461 tcattatttt taactggatt aacaataatc ttaggcaatg gaattccact ttcctcaaag
     2521 aaattccaac gcataa
//
LOCUS       DAZGLJ010000362         2505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000362 DAZGLJ010000000
VERSION     DAZGLJ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.368"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            298..1275
                     /locus_tag="ACHK49_004885"
                     /pseudo
     CDS             298..1275
                     /locus_tag="ACHK49_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1798..>2505
                     /locus_tag="ACHK49_004886"
     CDS             1798..>2505
                     /locus_tag="ACHK49_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytotoxin"
                     /protein_id="HFO9265353.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNLLTKTRTPRDNFEEGIKHPEHATI
                     PYDNDNESNKLLKAGKIAGNNNELLMEIKKESKSDHQIPLSDKFLKRKKRSPVAEDKV
                     QNSLTPENFVQKISLSDE"
BASE COUNT          809 a          527 c          544 g          625 t
ORIGIN      
        1 tgatcacaac tgaagatgct ttttacaatg aagaattatt gttaaaaaac gctttgttga
       61 atcaaccgaa ctgttgctga gttgcaggat cacatcacgt attttcctga aaacgcaggc
      121 tgcttagtag caaagcagaa gttcaaaatc accaactaaa tagctgcgcc gaatagtaga
      181 tcaatgaaag ggaactcagc ccggattgtg cgatctgatc aatcgccaaa aatccccccc
      241 taaccggact gagcgatgcc gatcatagca ccaattcccc gcgacgaatg acgcctgatg
      301 caaaaagcaa tctataaaac tcacgataaa aattatgccc gcaggctgac cgccatgctg
      361 atgctgcacc ggagcgctcg cgtcagtgac gttgccagaa cgctctgctg cgcccgttcc
      421 tccgtcaggc gctggattaa ctggttcacg ctgtcgagtg ttgaaggtct gaaatcatta
      481 cccgcaggac gttcccgccg ctggccgttt gagcatatct gcacattgtt tcgtgaactg
      541 ataaaacact ctcccggata ttttggctat cagcgttcac gctggagtac agaactgctg
      601 gcaataaaaa tcaacgagat aaccgagtgc cagttacatg ccggaaccgt tcgccgctgg
      661 ttgccattag tcgggcttgt gtggcgcagg gctgcgccaa ccctgcgtac ccgtaacccg
      721 cataaagcag caattcacaa agcactggac gaatgtagtg cggagcatcc ggtcttttat
      781 gaagatgaag tgtatatcta tcttaatccc aaaaccggtg cggactggca acttcgcaga
      841 cagcaaaaac atgtggtaac accggggcag aatgaaaaat actatctggc tggtgcttta
      901 catagtggaa cgggcaaagt cagctatgtg ggtggcaaca gcaaaagctc agccttattt
      961 atcagtctgt tgaaacatct gaaattgaca taccgacggg ataaacctat cacgctcata
     1021 gtggacaact acatcatcca taaaagtcgg gaaacacagc gctggctgaa agaaaatccg
     1081 aagttcagag tgatttatca gccagtttac tcgccgtggg tgaatcatgt tgagcggtta
     1141 tagcaggcgc ttcacgatac gataacgcgc aatcatcagt gccgttcaat gtggtaactg
     1201 cttaaaaagg ttcattattt tatggaaacc gtgagcccat tccccggtgg taaatatggg
     1261 ctgttaaaag tgtagcggta ttcggcgcag ctatttagga tggtcaggtc gcagaagttc
     1321 tgtgccacta cagtgcgtgt actaaacaaa atgacgaggg cacgaatgtc ataatacgtg
     1381 cgtattacct gcagtagcta ccaactgagg gggcttatcc caaacctgat ttattcaaca
     1441 aagctggact ggacttatcc ttactctgaa ttattttcca gacatatgca tcatgtactg
     1501 gctcgcttgg ttcaggaaat acaggagcaa atctacagct attagattta ttcctatttg
     1561 tatgcaaagt cgcattgcat aatgcggagc tatgttacag atacgcctga cgtgcgtagt
     1621 aaaattataa agcaacatat atatacaagg agaaaccgag aaaaataaat gagtcgtatg
     1681 ttgggtagca tcgagataaa tattttttta tcacattagc accttggtta agggtgttaa
     1741 taataagatt ttctcactta ttaaaacata aataattttt taagcggaaa tgtaattatg
     1801 agactgccag agaaagttct ttttcctcct gtcactagtg gcctgtcagg gcaggaaaaa
     1861 caaaaaaaac cgaagagcat taccggattt caggaaaatt atcaacgcaa tatcaggcca
     1921 atcaaaacag catcagaagc ccgactacgc ttctttgata aaatggtttc gaaagaaaac
     1981 tctctggaag atgttgtttc tttaggtgaa atgattcaga aggaaattta tgggcatgaa
     2041 caaagaacat tttcaccagt tcatcataca ggtaactgga aatcatcatt gttacacaac
     2101 gcgctccttg gtctggcaaa tgtttataat ggcttacggg aaacagaata ccctaacact
     2161 ttcaacagag atggtataaa aagtactaac tcttttagag ataacttatt gacaaaaaca
     2221 agaactccca gagataattt tgaggaagga ataaaacatc ctgaacatgc aacaatacca
     2281 tatgacaacg acaatgaaag taataaattg ctaaaagcag gaaagatagc tggtaacaat
     2341 aacgagctgt tgatggaaat aaaaaaggaa tccaaaagcg accatcaaat ccccctgtca
     2401 gataagttcc tgaaaaggaa aaaacggtct cctgtagctg aagataaagt tcaaaactcg
     2461 ttaacaccag aaaattttgt tcagaaaatt tcacttagtg atgag
//
LOCUS       DAZGLJ010000363         2504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000363 DAZGLJ010000000
VERSION     DAZGLJ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.369"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(201..1559)
                     /locus_tag="ACHK49_004887"
     CDS             complement(201..1559)
                     /locus_tag="ACHK49_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFO9265354.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            1841..1972
                     /locus_tag="ACHK49_004888"
                     /pseudo
     CDS             1841..1972
                     /locus_tag="ACHK49_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2292..>2504)
                     /locus_tag="ACHK49_004889"
     CDS             complement(2292..>2504)
                     /locus_tag="ACHK49_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFO9265355.1"
                     /translation="NILYEANSQFFYERNVIEEAVNALFCEREIINNKNIIKKLMFFL
                     SDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          614 a          628 c          586 g          676 t
ORIGIN      
        1 tttgttcgga ctgcttatgg cctttttcca ctggtgtagc aggaaagtta accggctgcc
       61 accatgagat gacgtgtaaa ccgtgcaggc atgttttcac gtggtgacgc tttcattggg
      121 ggtctacgcc ggaaccccaa tattgttcac atacatgaat ttgaaaaact aagcgatact
      181 gctgtccgta ccttataaac ttatttcaac gcttccggca gcgtcacaat ccacagttca
      241 ctgtcagact ccggtttctc cagtggcttc actctgaggg tcgtttcaac ctgcttgtcg
      301 tcaagcgtca gctttccttc cgcgttcacc cgataatcct tgaatgtctt gccctcgacc
      361 ggatgttgca tcgccacctt cacgccaaag tcgttatcgt tcgccaccgc cagcgttttg
      421 ctgtcgatca gcgccaaccc ttcagccttc tcctgctgcc agcccaagga gcgcagatca
      481 acaacctgcg ttttctgcgc aagggtaatg ccgcgctgtg acagggtttt ctcatcatca
      541 aactccggat actcacccgg cttgtcgaag cctgacaggt cgctcgcctt attgagatcg
      601 acctcgtaaa tcaggttacg cattctgttg tttttatctc ttccctgttc gatcagcagg
      661 atgtgctggt tatcgagcgc cacgatgtct ccgattttag cgtcgctgtt tttgctgtag
      721 gccgcgctgt cgataggata accgtacatc gcggtttttc cgcttgccgg atcgaagctc
      781 accagacgcg taaacagcgc ctttttcttg ctcttagcgt cgatatccag cgtactttgc
      841 acggcgacaa taatacgccc gtccggcata cgggtgaggc cttcaaagcc gcggttcgcc
      901 tgacgccatt tgaggatatt tggcaaaccg cccgcaatcg ctttttcccc ttccgccgcc
      961 tgcggaccgt ggattgccag gattttccct ttactgtcga tgttaatcag gaacgggcca
     1021 tactcatcgc acagccagta acctccttta ccatccggag tgatcccttc cgtatccagc
     1081 ccgcgattat cccccttcag tctatgtaac gtgtcgctga aggccacttc attggtggag
     1141 ccaatcacgt cgcttgccag cggcagaccg ttgatcgcgc ctttatcgtc atgcagagga
     1201 cggggatcga tggcctccgc tttgccgttt tgtacacgaa tcgtcatcag cagcggcgcg
     1261 aaatctggcg taacgaaaat tttagtttcg tttttcccct ctttcggtga atccgcgttc
     1321 ggaccgcgat cggtaatggt tgcgaatgtc agggcatcgc cctgtttacc ggtgaacagc
     1381 aggccagaac ctatcccaac cggaaggccg ttcgggaatg cactggcaaa tgcgccggca
     1441 tagttcacgt gcgtgccttc tggaaaactg acgacatagc gctcagccgt aggttgtgcc
     1501 gccagggcag agaaagagag cgtacaaccg attagcacgg gaataatttt tcttttcata
     1561 ttgttagcat ccataaagtg aagatcacac tacaccataa agaagaaacg agtcaggaaa
     1621 atgacatcga atggcaacat gcgcgggcct gaggccaaag aaatataaaa cactgttgtt
     1681 atttgtaaca acggaaacat ttacggaaat tatttgtctt tcttcgtgat caaaaatgct
     1741 gtgtcttgtt ggattggagt gatgagggct gcgtttagga aacgccctat attgtacggt
     1801 tgcattatcg cggtaggggt aatgcacgtg tcagtatgat gagctgacca cactcacgtg
     1861 ggtggactgg tataacaatc gacgattgct ggaaaggcag ggccatattc caccggcaga
     1921 agcagaaaaa gcttattatg cttccatcgg aaacgatgat ctggcagcct gagttcacag
     1981 ataaaacact ctccaggaaa cccggggcgg ttcaattgct ggataagatc ataacgatac
     2041 accgggttaa acaggtggtc actgcttcac tgaagctgcg tgattatgat ggtcaggtag
     2101 cagaagctat gcccatgata ccaaaatgac gaaagccgaa atgtctgaaa gcgtgtgtat
     2161 tacctgaaaa tatgaccggc tccggggtac gtaaccgatt caacaaagcc aatcgatatt
     2221 acaaaacgag agggactata tatttcgttt tcagataaga ctccttgaga gattatcttt
     2281 tatgtaaaat ctcaaatatc acctgatgta atgtcaatga taatattaag tgcgctctga
     2341 acaacatctt tcttggtgtg attaacatct gacaaaaaga acatcagctt ctttattatg
     2401 ttcttgttgt ttattatttc acgctcacag aataaagcgt tgactgcctc ttctatgaca
     2461 tttctctcgt aaaaaaattg agagttagct tcatataaaa tatt
//
LOCUS       DAZGLJ010000364         2467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000364 DAZGLJ010000000
VERSION     DAZGLJ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.372"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..260
                     /locus_tag="ACHK49_004890"
     CDS             <1..260
                     /locus_tag="ACHK49_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO9265356.1"
                     /translation="LIIWYQSDAFLPPESIFTLTHTGIMLNNKVLPVTIYNIVPFNKT
                     FWNLIKNSQECPTNTDNVLNECFNNRCTLQICPYGLKQQNP"
     gene            331..447
                     /locus_tag="ACHK49_004891"
                     /pseudo
     CDS             331..447
                     /locus_tag="ACHK49_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(541..672)
                     /locus_tag="ACHK49_004892"
     CDS             complement(541..672)
                     /locus_tag="ACHK49_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO9265357.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1172..1248)
                     /locus_tag="ACHK49_004893"
     tRNA            complement(1172..1248)
                     /locus_tag="ACHK49_004893"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1212..1214),aa:Arg,seq:tct)
     gene            complement(1346..1421)
                     /locus_tag="ACHK49_004894"
     tRNA            complement(1346..1421)
                     /locus_tag="ACHK49_004894"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1386..1388),aa:Ile,seq:cat)
     gene            complement(1471..>2467)
                     /locus_tag="ACHK49_004895"
     CDS             complement(1471..>2467)
                     /locus_tag="ACHK49_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO9265358.1"
                     /translation="EFIQTLPENSVDLIVTDPPYFKVKPEGWDNQWEGDDDYLKWLDQ
                     CLAQFWRVLKPAGSLYLFFGHRLASDTEIMMRERFNVLNHIIWAKPSGRWNGCNKESL
                     RAYFPATERILFAEHYQGPYRPKDDGYVAQGRELKQHVMAPLISYFRDARKSLGITSK
                     QIAEATGKKNMASHWFGTSQWQLPNEGNYNKLQALFARVAAEKHQRGELEKPHHQLVS
                     TYSELNRQYTELLSEYKNLRRYFGVTAQVPYTDVWTHKPVQYYPGKHPCEKPAEMLQQ
                     IINASSRPGDLVADFFMGSGSTVKAAMALGRCAIGVELETGRFEQTVREVQDLIV"
BASE COUNT          683 a          665 c          491 g          628 t
ORIGIN      
        1 aattaataat ctggtatcaa tcggatgctt tcttacctcc tgaaagtata tttacactga
       61 ctcacacagg tatcatgctc aataacaaag tgctacctgt aaccatttac aacatagtac
      121 cattcaataa aacattctgg aatttaatca aaaacagcca ggaatgccct acaaatacag
      181 ataacgtatt gaatgaatgc tttaataacc gttgcactct gcaaatatgt ccttatgggc
      241 taaaacaaca aaatccataa ggagtttact cacatctgac aaaatcaata taaacagccc
      301 ctccggagag gggctggaga gtggcgctat gtgccattgc atggtgccgg gtgcctcccg
      361 gtgaattcag taccagcacc tgaatccgcg attatcccat atacctactc gctgattgcc
      421 cctccgcaca gggggattca ccatgccagt ttcttttaac aaactccccg caaaccagac
      481 aacagtcaac cgcctgaatt gtgaagtatt taaaaatttc tcccgctaac tgatacccgg
      541 ctaacagtct ggcgttttct ttttcagcaa cgggaaagca acaaccacca cacccgccac
      601 cagcacaccg tcagccagca ctgacattat ccggctgctg caatgccatt cacaaaaaca
      661 gtaagcaatc actttttacc gtaacaggtg ataatccaga tatgtatcta ccccagatga
      721 gtaatccgaa gttcatccat accacaggtc ctggctattc tgttgtactc ctgaacaaga
      781 gcaaataatt ctgaattagc aaccatgaac tcatcgcaaa ccctctgtat agcatcacta
      841 ttcagaataa taacgtctct tcccaaaaga cgatcaggag tacagaacaa aactgtcaaa
      901 cggctgaagg cctttgctcg tgctgcattg actatatcaa tacgctgcct aaggatgaaa
      961 caccccgacg cctcatcaat attcactcta cccacaccat atgaatgata aatatttaat
     1021 actgaaaaaa ccattagacc gtataacaaa cactcaatca atacttaaca gaacttttat
     1081 ttttgacaaa catataatat tttcaacaat atactgagcc aggtatattc cagtataagg
     1141 ctctgccgga agaaatctgg aagaatgaat atggcgcgtt gtactggatt cgaaccagtg
     1201 accgattgct tagaaggcaa ttgctctgtc cggctgagct aacaacgcat aatgcagata
     1261 atggattgcc atcggggacc cgggccccac acagccagtt tcgaaagctg acgctctctg
     1321 ccgatgagct aatggcggta tgtgatggtg gcccttgctg gatttgaacc agcgacctgg
     1381 cgattatgag tcgctcgctc tcaccactga gctaaagggc cggtagcaga ataataatgg
     1441 tgcgtaatta attctgcaat cccatccgtt tcaaacgatt aaatcctgaa cttccctgac
     1501 tgtctgttca aaacgtcctg tctccagctc aacaccaatc gcacaacgcc ccagtgccat
     1561 cgccgctttt accgttgaac ctgaacccat aaaaaaatct gcaaccaggt ctcccggacg
     1621 actgctcgcg ttgattatct gctgcagcat ttctgccggt ttttcgcacg gatgtttccc
     1681 tggatagtac tgcaccggtt tatgcgtcca gacatcggtg tacggaacct gcgccgtcac
     1741 accgaaatac cgccgcaaat ttttatattc actcagcagt tccgtatact gccggttcag
     1801 ctcactgtat gtgctgacca gctggtggtg tggcttttcc agttccccgc gctgatgttt
     1861 ttctgccgca acacgcgcaa acaacgcctg caatttgttg taattaccct cgttcggtaa
     1921 ctgccactga ctggtaccaa accagtgcga agccatgttt ttctttccgg tggcttccgc
     1981 tatctgtttt gacgttattc ccagtgattt acgcgcatca cgaaagtaag aaatcagcgg
     2041 ggccatgacg tgctgtttta gctcgcgccc ctgtgccaca taaccatcat ctttcgggcg
     2101 atacggtccc tgataatgtt ctgcaaacag aatgcgctct gttgccggaa aatacgcccg
     2161 cagactttcc ttattgcacc cgttccagcg tccggacggc ttcgcccaga taatatggtt
     2221 cagcacatta aagcgctcac gcatcatgat ttcggtgtca gatgccaggc gatgaccaaa
     2281 gaacaggtaa agacttccgg caggcttcag tacccgccag aactgcgcca gacactggtc
     2341 cagccatttc aggtaatcat cgtcgccctc ccactggtta tcccagccct cgggcttcac
     2401 tttaaagtat ggcgggtctg tgactatcag atcgacagag ttttccggta aggtctggat
     2461 aaattcc
//
LOCUS       DAZGLJ010000365         2456 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000365 DAZGLJ010000000
VERSION     DAZGLJ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.373"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..417
                     /locus_tag="ACHK49_004896"
     CDS             <1..417
                     /locus_tag="ACHK49_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO9265359.1"
                     /translation="GTFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIR
                     TDNRPENLRECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCF
                     DDLELAVLVAEEARDKYYGDFSGNERSTYANLSKEM"
     gene            392..994
                     /locus_tag="ACHK49_004897"
     CDS             392..994
                     /locus_tag="ACHK49_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO9265360.1"
                     /translation="MRIYRRKCKCCNEWFIPKYQNQYWCNEICGTKIALERRSKEREK
                     AEKAAEKKLRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISCG
                     TLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIGQEA
                     VEEIESNHNRYRWTVEECRAIKAEYQQKLKKLRNSRSEVA"
     gene            991..1656
                     /locus_tag="ACHK49_004898"
     CDS             991..1656
                     /locus_tag="ACHK49_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO9265361.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            1653..2276
                     /locus_tag="ACHK49_004899"
     CDS             1653..2276
                     /locus_tag="ACHK49_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO9265362.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
BASE COUNT          780 a          510 c          609 g          557 t
ORIGIN      
        1 ggaacttttg attccaacgg ttattcaatg atcatgatag atgggcgtag atataaaact
       61 cacgtcttag ttttttacat aactcataat cgttggcctg ctggtcaaat tgaccacgtt
      121 aatggaatta ggaccgacaa taggccagaa aatttaagag aatgcctgcc aatagaaaat
      181 tcaagaaata taaggatccg aaagaatagc aaatcaggtt gcagaggggt tacttggcac
      241 aaacgacaga aaaaatggaa tgttaggcta ggattccatg gcaagagtaa acacttcgga
      301 tgctttgatg atctggagtt agcggtacta gttgctgaag aagcccgaga taagtattac
      361 ggtgattttt ccggcaacga aaggagcact tatgcgaatc tatcgaagga aatgtaaatg
      421 ttgcaatgaa tggtttatac caaaatatca aaatcaatat tggtgtaatg agatttgtgg
      481 aaccaagata gcactcgaac gacgaagtaa agaacgcgaa aaagcggaaa aagcagcaga
      541 gaagaaacta cgacgagagg agcagaaaca gaaagataaa ctgaagattc gaaaactcgc
      601 cttaaagccc cgcagttact ggattaaaca agcccaacaa gccgtaaacg ccttcatcag
      661 agaaagagac cgcgacttac catgtatctc gtgcggaacg ctcacgtctg ctcagtggga
      721 tgccggacat taccggacaa ctgctgcggc acctcaactc cgatttgatg aacgcaatat
      781 tcacaagcaa tgcgtggtgt gcaaccagca caaaagcgga aatctcgttc cgtatcgcgt
      841 cgaactgatt agccgcatcg ggcaggaagc agtagaggaa atcgaatcaa accataaccg
      901 ctatcgctgg actgtcgaag agtgcagggc catcaaggcg gagtatcaac agaaacttaa
      961 aaaactgcga aacagcagaa gtgaggttgc atgaatatct acgaaagaat tgatggcagc
     1021 aaataccgaa atatttgggt agttggcgat ctgcacggat gctacacgaa cctgatgaaa
     1081 aaactggaga cgataggatt cgacaccaaa aaagacctgc ttatctcggt gggcgatttg
     1141 gttgatcgcg gtacagagaa cgtagaatgc ctggaattaa tcacattccc ctggttcaga
     1201 gctgtacgtg gaaaccatga gcaaatgatg attgatggct tatcagagcg tggaaacgtc
     1261 aatcactggc tgcttaatgg cggtggctgg ttctttaatc tcgattacga caaagaaatt
     1321 ctggctaaag ctcttgccca taaagcagat gaacttccgt taatcatcga actggtgagt
     1381 aaaggaaaaa aatatgtcat ctgccacgcc gattatcctt gtgataaata cgagtttgga
     1441 aagccagttg atcatcagca ggtaatctgg aaccgcgaac gaatcagcaa ctcacaagac
     1501 gggatcgtga aagaaatcaa aggcgcggac acgttcatct ttggtcatac gccagcagtg
     1561 aaaccactca aatttgccaa ccagatgtat atcgatactg gcgcagtgtt ctgcggaaac
     1621 ctcacattga ttcaggtaca gggagaaggc gcatgagact cgaaagcgta gctaaatttc
     1681 attcgccaaa aagcccgatg atgagcgatt caccacgggc tacggcttct gactctcttt
     1741 ctggtactga tgtgatggct gctatgggga tggcgcaatc acaagccgga ttcggaatgg
     1801 ctgcattctg cggtaagcat gaactcagcc agaacgacaa acaaaaggct atcaactatc
     1861 tgatgcaatt tgcacacaag gtatcgggga aataccgtgg tgtggcaaag ctcgaaggaa
     1921 atactaaggc aaaggtactg caagtgctcg caacattcgc ttatgcggat tattgccgta
     1981 gtgccgctac gccgggcgca agatgcagag attgccacgg tacaggccgt gcggttgata
     2041 tagccaaaac agagcagtgg gggatagttg ctgagaaaga gtgcggaaga tgtaaaggcg
     2101 tcggttattc aagaatgcca gcaagcgccg catatcgcgc tgtgacgatg ctaatcccaa
     2161 accttaccca acccacctgg tcacgcactg ttaagccgct gtatgacgct ctggttgtgc
     2221 aatgccacaa ggaagagtca atcgcagaca acattttgaa tgcgatcaca cgttagcgcc
     2281 atgattgcca cggatggcaa catattaacg gcataatatt gactttttga ataactttgg
     2341 ggaaacttga caccaataat gggcgttttt tacatgtcat tgatgagtct caataacctg
     2401 ccgccgagta gtttttatgc tctgaattgt atttgtgtag taaacatgct gactgc
//
LOCUS       DAZGLJ010000366         2447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000366 DAZGLJ010000000
VERSION     DAZGLJ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.374"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..69)
                     /locus_tag="ACHK49_004900"
     CDS             complement(<1..69)
                     /locus_tag="ACHK49_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310734.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265363.1"
                     /translation="MSFEDSEKASTVTLQQHYNFVMN"
     gene            91..504
                     /gene="hiuH"
                     /locus_tag="ACHK49_004901"
     CDS             91..504
                     /gene="hiuH"
                     /locus_tag="ACHK49_004901"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO9265364.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            613..1617
                     /gene="msrP"
                     /locus_tag="ACHK49_004902"
     CDS             613..1617
                     /gene="msrP"
                     /locus_tag="ACHK49_004902"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO9265365.1"
                     /translation="MKKNQFLKESDVTAESVFFMTRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNDLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYAEQVASLYRGLDLRENF
                     "
     gene            1618..2253
                     /gene="msrQ"
                     /locus_tag="ACHK49_004903"
     CDS             1618..2253
                     /gene="msrQ"
                     /locus_tag="ACHK49_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO9265366.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQVHNKLSV"
BASE COUNT          632 a          585 c          579 g          651 t
ORIGIN      
        1 gttcattaca aaattgtaat gctgctgtaa ggttaccgtg gacgcttttt cgctatcctc
       61 aaaactcatt cacatgacaa ggatataaac atgttaaagc gttatttagt actctccgta
      121 gtaacggctg cattttcatt accttctttg gtctatgctg cacaacaaaa cattcttagt
      181 gtgcacattt tgaaccagca aacaggaaaa cccgctgccg acgtgacagt cactcttgaa
      241 aagaaagcgg acaacggctg gttacaactt aataccgcca aaacagataa ggatggacga
      301 attaaggcac tgtggcccga gcaaactgca actacgggcg attaccgtgt cgtatttaaa
      361 accggggact atttcaagaa acaaaatctt gaaagtttct tccctgagat ccccgttgaa
      421 tttcatatta ataaagtgaa cgagcattat catgtgcctt tattacttag ccaatatggg
      481 tattcaacct atcgtggcag ttaatgcata aatatgtaaa agcagatctc tgctactgtt
      541 agggaggaca cttcctgaca gattaacagt aaacggctct tgctggctaa agacaaaaaa
      601 gtgtgatggc ttatgaaaaa gaatcaattt ttaaaagaat cagatgttac ggccgagtcg
      661 gtattcttta tgacgcgtcg gcaggtgtta aaagcactgg gcatcagcgc ggctgcactt
      721 tctttgcctc acgctgcgca tgccgatctg cttagctggt tcaaagggaa tgatcgccca
      781 cccgcccccg ccggaaaacc gttggagttc agcaagcctg ccgcctggca aaatgacctg
      841 ccactgacgc cagcagataa agtctccggt tataacaact tctatgaatt cgggctggat
      901 aaagccgatc ccgccgctaa tgctggtagc ctgaaaaccg atccatggac actgaaaatc
      961 agcggcgaag tggcaaaacc attgaccctc gatcacgatg atttaacccg tcgctttccg
     1021 ctggaagagc gtatttatcg tatgcgctgc gtggaagcat ggtcgatggt ggtgccgtgg
     1081 attggttttc cgctgcacaa attgctggcg cttgccgaac ccaccagcaa tgcgaagtat
     1141 gtcgctttcg aaacaattta tgcaccggaa caaatgccag gccagcagga ccgctttatc
     1201 ggtggcgggc tgaaatatcc ttatgtcgaa ggattgcgtc tcgacgaagc aatgcatccg
     1261 ctcacactga tgaccgtggg tgtttatggc aaggcgttac cgccacaaaa tggcgcgccg
     1321 gtgcgactga ttgtgccgtg gaaatatggc tttaaaggga ttaaatcgat agtcagtatt
     1381 aagctgaccc gcgagcgtcc gccaaccacc tggaatctgg cagcgcctga cgaatacggt
     1441 ttttacgcca acgttaatcc gcatgttgat cacccgcgct ggtcacaggc taccgaacga
     1501 tttattggtt caggcggcat cctcgatgta cagcgccagc caacgctact gtttaatggt
     1561 tacgccgagc aggtggcatc gctgtatcgt ggcctggatt tgcgggagaa tttctaaatg
     1621 cgtctgacag caaaacaggt gacatggctg aaagtttgcc tgcatcttgc cggattgttg
     1681 ccgtttctct ggctggtctg ggcgattaat cacggtggat tgggtgccga tccggtgaaa
     1741 gatattcagc attttactgg tcgcactgca ctgaaatttt tgctggcgac cttgttaatc
     1801 acacctctgg cacgctacgc aaaacagccg ttattgatac gcactcgccg cctgttaggc
     1861 ttatggtgct ttgcctgggc gacactgcat ttaaccagtt acgcattgct ggagctgggc
     1921 gtgaataatc tggcgttatt aggtaaggag ttaattaccc ggccttattt aacgttaggt
     1981 attatcagct gggtaatttt gcttgcgtta gcgttcactt caacccaggc gatgcagcga
     2041 aaactcggta aacactggca acagttgcat aacttcgtct atcttgtcgc gatcctggcc
     2101 ccaatacatt atttgtggtc agtgaagatt atttcaccgc agccactcat ctacgctggg
     2161 ctggcggtac tgcttttagc cttacggtat aagaagttgc tttccctttt taaccggtta
     2221 cgcaagcagg ttcacaacaa attatctgta taacttcata ttgccgacaa agtacgcgtt
     2281 atccctgccg gatgcggcgc gagcgcccta tccggcctac gcagatcaca tcaagttatg
     2341 tttcgtttgt catcaacctc aaatcatcga cataatttat tttatttcaa taagatagat
     2401 aagcacacct gagagaacta ttctcttgtc tcctaagagt tgttgca
//
LOCUS       DAZGLJ010000367         2416 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000367 DAZGLJ010000000
VERSION     DAZGLJ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.375"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..414
                     /gene="ycgY"
                     /locus_tag="ACHK49_004904"
     CDS             <1..414
                     /gene="ycgY"
                     /locus_tag="ACHK49_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFO9265367.1"
                     /translation="SSQSGVKNGADSEAVLAALIEHLELINPSGRLSQNTRSAMLQLR
                     EEEWSEFFFWLLNSLECLDYVIINLAPESKKTLMSEHRNNIQVAIDALYRQRRCKSPG
                     DESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(493..2190)
                     /gene="treA"
                     /locus_tag="ACHK49_004905"
     CDS             complement(493..2190)
                     /gene="treA"
                     /locus_tag="ACHK49_004905"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO9265368.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
BASE COUNT          564 a          574 c          619 g          659 t
ORIGIN      
        1 agtagccagt caggcgttaa aaatggggcg gatagtgagg cggttttggc tgcgcttatt
       61 gaacatttag aattgatcaa tccttctggc agattaagcc aaaatacccg ttcggcaatg
      121 cttcagttac gcgaagagga atggtctgaa ttcttttttt ggttgctaaa ttctctggag
      181 tgtctggatt atgtgattat taatttagct ccagaaagca aaaaaacgtt gatgagtgaa
      241 caccgtaaca atattcaggt agcaatcgat gctttatata ggcaacgtcg gtgtaaatca
      301 ccgggtgatg aaagtgaaac acttacccgt agaaatgacg ctatcttcgg caaccacgta
      361 tggcaaacat ttgcgcaata cttcccgcca ggacttgaaa aaccgtctgt gtagaaaaca
      421 ccatagctat ggtgttatct gccgacaacg ccatccggag aagccgaatg acgttgtcgg
      481 agtaagcgct ggttaaggtg tgggttgtgc ctctttggtt gagggttgcg tcgttgctga
      541 cttaacggtc ggacgcgtcg ccggaacatt gtcacacggt tgctctttcg ggcagatcaa
      601 atccagcatt ttcagcgtca cgccattggt ccagccaaag ccatcctgta atggatattc
      661 gccaccgccg ccccccgttc cggtggtgct gacatcatat ttttccacca gctttttctc
      721 ccggtcatag gtgtgctgaa cattggtcag gaagtgccag ctaatgtcca tcgccacctc
      781 tttttgcccg tagttttgta atccttctgt cgcgacccac tgtaacggtg cccagccatt
      841 tggcgcatcc cattgttgcc cacttttcac cgacgtggtg ttcaggccgc cgggttgcag
      901 cagatgtgtt ttcgtcgccg tcgccatttt gttggcgcga tctttcgctg ccgcattgac
      961 gtacagcggg aacagggcgg ccgcggttaa ctgattgcgc actttatgac ttttcaggtc
     1021 gtaatcggca taccagcctt gttgatcgtt ccacatgtat ttttcgatcc ctttttgacg
     1081 ggcatttgcc agcgtttcgt actggtttgc catcgcgtta tctccggcag ctttgctggc
     1141 gcgggcgagg attttttcca ttttaaacat caggctgttc agatcgaccg gtacgatgct
     1201 ggtggtgcgt aaggtattta gttgatgcgg gttgtccatc cagcgcgagc tgaaatccca
     1261 gccagacgca gcggcagagc gcaggtcgcg gtaaatttca gtggcaggtc gattcggatt
     1321 gcttttggcg gtggcaatat cttccaccca tgactctggt cgtggcgtat cgcgatcgtc
     1381 ccagtagcgg ttgagaaggg taccatcctg aagttttaca acgcgttttt cctgttgtcc
     1441 ggcttgcagg ttttcaacac cgtccatcca gtaagcatat tctttttgca tttgcggcag
     1501 gtattgcttc aacgcggcat cgccttcatg ctgcgccagt aactctacca tcagggcaaa
     1561 gaagggcggt tgcgagcggc ttaaatagta actgcggttg ccgttgggaa tatgaccgta
     1621 agtgtctatt tcatgagcaa aattggccac catatccgcg actttatccc agtgaccgct
     1681 ttcggcaagt cctaacatgg tgaagtaact gtcccagtaa tatacctcgc gaaaacgtcc
     1741 gcccggcacg acataaggtt ccggcagcgg taacagagaa tcccattttt cggtgttttc
     1801 ggtagaacgc gttaataccg gccaaagtcc gtcaatatgt tcgcgcagtg actgcccctc
     1861 tggcggaaca tatttctcgc cctctttcgg cagggtgaaa ttgacgttaa cgaaatggcg
     1921 cagatcaaat ccgctctggt tttgctgcat ccgataatca gcaaggatca tcagcggatc
     1981 gctgttcggc acggcatcgg caaaggtttt ttggtccgga aaaagtttgg cgttttgcac
     2041 atcattaaac agcggcccta ataaaatatc aggcggctgt ggtgttaccg gtgtttcttc
     2101 tgcctgcacc gatagcgcag cgaaacacaa aaagatacag gctggaatta acgccatttt
     2161 ttgcgggcga gaaggtgcgg gggatttcat caatcattct cctttggcga aaccgaataa
     2221 aacggtattc aagtatcaga aaaccttagt tcaggatcgc actagctgca tgatcgaaat
     2281 ctcattctgc gaactgtcag gcattttaga aaagggaatt aaagggataa ataacggtga
     2341 aatagagtga tcaaataacg tctgtttatt agccagtcag cgacgttggg ttcgcgatta
     2401 gcgaagaaaa atcatc
//
LOCUS       DAZGLJ010000368         2408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000368 DAZGLJ010000000
VERSION     DAZGLJ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.376"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            546..773
                     /gene="ykgL"
                     /locus_tag="ACHK49_004906"
     CDS             546..773
                     /gene="ykgL"
                     /locus_tag="ACHK49_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HFO9265369.1"
                     /translation="MQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(808..948)
                     /gene="ykgO"
                     /locus_tag="ACHK49_004907"
     CDS             complement(808..948)
                     /gene="ykgO"
                     /locus_tag="ACHK49_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO9265370.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(948..1211)
                     /gene="ykgM"
                     /locus_tag="ACHK49_004908"
     CDS             complement(948..1211)
                     /gene="ykgM"
                     /locus_tag="ACHK49_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO9265371.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVTSEGNVARFTQRFGRFVSTKKGA"
     gene            complement(1575..1676)
                     /gene="ykgR"
                     /locus_tag="ACHK49_004909"
     CDS             complement(1575..1676)
                     /gene="ykgR"
                     /locus_tag="ACHK49_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFO9265372.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            complement(2150..2239)
                     /locus_tag="ACHK49_004910"
                     /pseudo
     CDS             complement(2150..2239)
                     /locus_tag="ACHK49_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          711 a          447 c          532 g          718 t
ORIGIN      
        1 aagaagaaaa agacatttgg tttcataagg ttattggcaa tataacgttc aattcttact
       61 tgcattttat attggtcagg aactttgccc tgaagtgtta ggaatagtct taatgcgctg
      121 tgtgatggac tagtaaaaaa acaagtttta agggtaaggg atagaatata aaactataat
      181 gagctgtcat tgaagatgat ttgccaatca gtaagaaatt ttaatggatt aacatgccgc
      241 acaaaatggt tataggtgtt cattacaata agtgatggaa aattgaacgg gcgtatgtac
      301 gtcatctgtt cggtaagccc ccaagagcca gattaagtga agttaaacga aggctgccag
      361 ctgtcacagg aacgatggcc tctttgtcag ggcactgagt gcagttatct gtgcgcgtta
      421 ctctaactgg cactcgaacg tcggctacgg gttaccggtg gatcagactg ttcctgtggc
      481 gtaaacgttt ttatgttagt accaacagat ttaccacatt tatcatctta acagtttgag
      541 tgaaaatgca ggctgcgccg caaagggagg agactgagtg gcgtgttcag tctaaaagag
      601 ggttgatgcc agcatacagg ggcgaagctg gtcaacaagt caacattaag ataatggaat
      661 attcggagag aaacgtcagg cagttggcct caaatgaaca ggaagaatat atccccagga
      721 aaattaatgt tggtgttata aacacaccaa cattaatccg atctgattat taatcagaca
      781 aatatcgtta attcgtcgaa ttttgaatca acgttttttc ttacgaccct gaacggcctt
      841 aaaacgtgga ttagatttac aaatcacata caaccgtcct tttcgcttca caatctgaca
      901 gtctggatgg cgttcttttg cggtacgcag agagttaaga actttcatca cgcccccttt
      961 ttcgtgctaa caaaacgacc aaaacgttgg gtgaatcgtg caacatttcc ttctgatgtc
     1021 actgttctca gcttccctgt atagaacggg tgcgatttag aagagacatc aattgtcacg
     1081 tacggatacg ttacgccatc cagctcaatc tcacggtctg ttttgatagt cgagccgatt
     1141 ttaaagtact catcaacact ggtgtcgtgg aacaccacag tacgatactc aggatggata
     1201 ttgggcttca tcatttttac ctgttatgtt ataacataac cgtaaagata catgctcatc
     1261 cggatcattt caacacccaa tccctgtgcc catttgtggt atgtggaatt tccccaacgg
     1321 ggaagtctgg cattgttagc agccgttggc gacgctccca taaacgcctg gatgagtgat
     1381 tgatgatggt tttgctgaga gtatggattt ttcgagaatg gaggttatct ccactttact
     1441 ggccgatgat ttatcgcagt atgattgatt acctgacagc gtgtattcct ctcccacgga
     1501 tataataatt acttacaaaa agggagagga tgcatatttt aaatatcact gaagtgaaca
     1561 gtttattgcc gttattaata gaaatggaga aataaatagg cgtattctac aattgcgaca
     1621 aaaacaacga tattaatcag tttatgactg atttgttgta ctttattctc tttcattggt
     1681 acttcctcgc tttaaaaaag agtgcacttc gtaagtgccc ttatataaat aacgagtttg
     1741 gtcaaccaat tttttgacat gtatcacaaa tttgaataga tgtattacat cagctatctt
     1801 ttattgcacc aacgtcattg atatatgtcg actgaagtca gttccgggaa tgagtctgat
     1861 ctcaagactg gcccagtccg ggcgttgatt ggcgctgagg agcatacctc atttcatcat
     1921 aatgtcgtat ctcctggggt gttatacaag atatcgttgt tggtaacctg ggaaaggaat
     1981 tgagttctat taaaccgtca actatgccgg atacatactg gattacactg caggcacgcc
     2041 ttatgagaga acgtgccgca gtgacgggtt aattatctga aagaatttgt gaggctgtat
     2101 cggttactca ttgatttgat agttttactc tcgggagaat aatagatatt taatccatta
     2161 acggaaacca gccagttcct ttcgatgcct gaatttgatc ccatagttta tccggaatgg
     2221 ttaattcatg aacaagttgt gttatcgttc atgagaagca taacgtaaag ggaaaagctc
     2281 gattagacgg cagaatttgt caggggttat gaacgaaatt cataaatctg tttgagtgtt
     2341 gcgatgggta gtgcaagttc gatatctccg caatttacag tccgatgaag gaaaatgaat
     2401 atccataa
//
LOCUS       DAZGLJ010000369         2400 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000369 DAZGLJ010000000
VERSION     DAZGLJ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.377"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..605
                     /locus_tag="ACHK49_004911"
     CDS             <1..605
                     /locus_tag="ACHK49_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265373.1"
                     /translation="ITDLIKNLNKTDIALYILAFLSAGYTFQYIYNDNIFSELWGVGL
                     FVTLIVLNIVFNLFFRLLFILSKMILMSRHIRKMQKKLKQTSVPDFIYYSIEKHNKYP
                     IYVLHMPSNNIIEIGYNIINTDLVIGEKIHFRTLSNRSLYFNLTQPPLIATIKDDVFC
                     TLHDYYNHNNETKSTIDNYISKIKDNHNTPWLLNNENVQE"
     gene            complement(1476..1616)
                     /locus_tag="ACHK49_004912"
     CDS             complement(1476..1616)
                     /locus_tag="ACHK49_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9265374.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(1918..2181)
                     /locus_tag="ACHK49_004913"
     CDS             complement(1918..2181)
                     /locus_tag="ACHK49_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO9265375.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
BASE COUNT          759 a          539 c          421 g          681 t
ORIGIN      
        1 taataaccga tctaataaaa aacctaaaca aaacagacat agcgttatat atattggcat
       61 ttttatcagc cggatataca ttccagtata tttacaatga caatatattt tcagaactat
      121 ggggagttgg cctgtttgtt actctgatag tactgaatat agtatttaat ctctttttta
      181 gactcttatt cattctctcg aaaatgatat taatgtcacg acatataaga aagatgcaaa
      241 aaaaactaaa acagacgtct gtgcctgatt ttatttatta ttcaatagaa aaacacaaca
      301 aatatcccat atatgtatta cacatgccat caaacaatat aattgaaata ggctacaata
      361 taataaacac cgatctggtc attggcgaaa aaattcattt caggacacta agcaatcgtt
      421 ctttatattt taacctcacc caacccccat taatcgccac aataaaagat gatgtgtttt
      481 gcactcttca tgattactat aaccacaata atgaaacgaa atccactatt gacaattaca
      541 tatcaaaaat aaaggataat cataataccc catggttatt aaacaacgaa aatgttcagg
      601 aataaaaaaa agagacaatt cccgagataa aacaaaagag cataaacata tgaaccggaa
      661 attctatttc aaaaatatcc tgtggggaat gtgccccatt ataatatcca tattgcatct
      721 gatatatgac acaccaacag aagcatcgat aaaagttgac ttatttctga tactcagtgg
      781 cctggccctt ccttgttcaa tatacctgac aaaaacaatc acactaaaat acacatccat
      841 atcattctgg aaaaaacctc ttttctggtc ttatggtata tatcattttc atcttgttta
      901 tataatgaca tgtatagttt tagcaatacc ttctgcaatt atcgttttat tctttcactt
      961 ttatcaaaac atcaccagaa actggaagtg acaaccgcaa taatgaacat aaaaaggcaa
     1021 atcagaatgg cagtaaatca ataaagtacc atttcatatg aatcatcaga aaaacggtgg
     1081 acgcgacatc cagcacgagg caaaacacag caacaaaata agccggatat cgcgccctgt
     1141 acttttctaa ctcaactccc ggcattgtgc aagttttaat cgcaggtgga ttcagagcca
     1201 cgcccccatt gattcagcct gaagacacgt atatccttgg agaaagcagg aatctcattg
     1261 ctgtgaaatt ccttacagaa tcttcagccc cattttatgg ggcttttttt ggtgccccca
     1321 cgctgttacc acactttcgg gatgcgaata aaaaagccca cagtttttac actgcggact
     1381 tttgtttttt ctttgtgatt tgtgtgcaag ggtgcgactt tcgaccgaac ctttggcaac
     1441 cggatagcgg ggactgttgt ccccgctgcg gtttcttacg gtttacactg caagaacgcc
     1501 gcaatttccg accacatgaa gcttaccctg aattcacacg gtgatgcgtg taacacccag
     1561 acggtcagca acatggtcag gcataacatg cccacaatga tcgttttttg cggcatagca
     1621 cttgctcctt ttacagagag gcgctaacct tccacttgct gaaggtagat ggtcagggcc
     1681 tcgggttaac gtttaagttg actcggggcc tttccacatc cggccttcag gtaatccttc
     1741 cagccgtcag ccgaaaggca cccgcgcgta atgtactggt tttcagagag gtccgcaatc
     1801 ttccggtgac gagatcatct gcacacgccc cggtgggacg ggctaacggt atattcaggt
     1861 gcaggatctc tctgtctggt gcgtttttaa attgtcaaac gcaccctggc ggcaaaatca
     1921 gccacctttt ttcgcatcaa caaaacgccc aaaacgctgg gtaaagcgtg caacattacc
     1981 ttctgaagcg acagttctca gtttacctgt ataaaacgga tgcgacttag aagaaacgtc
     2041 aattgtaaca tatgggtaag ttacaccatc cagttcaatt acccggtcag ttttgatcgt
     2101 cgaaccgatc ctgaagtact catcaatact ggtatcgtga aatactactg tacggtattc
     2161 cggatggata ttcggtttca caacagtttt ccccctttaa gtgaaaattc cgcataaagg
     2221 tacatattca gacggtcaga ttcaactgtc tgttcttata acctcactct ctttcagtat
     2281 tacaatatgt tatataatta taaactggac ttcccgaaaa aatccattaa ataagaagcg
     2341 agccgttgcg gaggcttgct ctctccttta acctcatgtg caatatccgt aacgcgctca
//
LOCUS       DAZGLJ010000370         2393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000370 DAZGLJ010000000
VERSION     DAZGLJ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.378"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            81..2348
                     /gene="adiA"
                     /locus_tag="ACHK49_004914"
     CDS             81..2348
                     /gene="adiA"
                     /locus_tag="ACHK49_004914"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO9265376.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNTGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT          604 a          656 c          595 g          538 t
ORIGIN      
        1 ttttcacgcg ctttacagcc cgaaaaggcc ggaagatact tgcccgcaac gaagattcct
       61 tcataaccgg gtaagcaatg atgaaagtat taattgttga aagcgagttt ctccatcaag
      121 acacctgggt cggtaacgcc gttgagcgtc tggcagatgc tttaagccag caaaatgtta
      181 ccgtgattaa atccacctcc tttgatgatg gttttgccat tctctcttca aacgaagcca
      241 ttgactgcct gatgttcagc tatcaaatgg aacatccgga cgaacatcaa aacgtcagac
      301 aattgatcgg taagcttcat gagcgccaac aaaacgtgcc ggtcttcctg ttgggcgatc
      361 gggaaaaagc cctcgccgca atggaccggg atctgctgga gcttgtcgat gaattcgcct
      421 ggattctgga agataccgcc gactttatcg ccggacgcgc cgttgccgcg atgacccgct
      481 accgccagca gctgttgccg ccactgttca gcgcgctgat gaaatatagt gacatccatg
      541 aatattcctg ggcagcgcca ggccaccagg gcggcgttgg ttttaccaaa acacccgccg
      601 gacgtttcta ccatgactac tatggtgaaa atctgttccg caccgacatg ggcatcgaac
      661 gaacttccct cggttctttg cttgaccata ctggcgcatt tggcgaaagc gaaaaatatg
      721 ccgcacgcgt atttggtgcc gatcgctcct ggtcggtagt cgtcggtact tccggctcta
      781 accgcaccat catgcaggct tgcatgaccg ataacgatgt cgtggtcgtt gaccgtaatt
      841 gccataaatc catcgaacaa ggcttgatgc tgacaggcgc gaaaccggtc tatatggtgc
      901 caagccgcaa ccgctacggc attatcgggc caatctatcc gcaggaaatg caacctgaaa
      961 ccttgcagaa gaaaatcagt gaaagcccgc tgaccaaaga caaagccggg caaaaaccgt
     1021 cttactgcgt ggtgaccaac tgcacctatg acggcgtgtg ttataacgct aaagaagcgc
     1081 aggatctgct ggaaaaaacc tccgatcgtc tgcactttga cgaagcctgg tacggctatg
     1141 cacgtttcaa cccgatctat gccgatcact atgccatgcg cggcgaacct ggcgatcaca
     1201 acggtcctac cgttttcgcc acccactcca cccacaaact gctgaatgcg ctgtcacagg
     1261 cttcttatat tcatgtacgt gaaggtcgtg gggcgattaa cttctcccgc ttcaaccagg
     1321 cctacatgat gcatgccacc acctccccgc tgtatgccat ctgcgcatcc aacgacgtgg
     1381 cggtgtcgat gatggacggc aacagcggcc tgtcactgac acaggaagtg attgacgaag
     1441 cggttgattt ccgtcaggcg atggcgcggc tatataaaga gttcaccgct gacggtagct
     1501 ggttcttcaa accgtggaac aaagaagtcg tcaccgaccc acaaaccggc aaaacctatg
     1561 actttgctga cgcaccaacc aaactgctga ccaccgttca ggactgctgg gtaatgcatc
     1621 cgggcgaaag ctggcacggc ttcaaagata ttccggataa ctggagtatg ctcgacccaa
     1681 ttaaagtcag catccttgct ccgggaatgg gtgaagatgg tgaactggaa gaaaccggtg
     1741 ttccggcggc gctggtcact gcctggcttg gtcgccacgg cattgtgcct acccgcacca
     1801 ctgacttcca aattatgttc ctgttctcta tgggcgtaac ccgtgggaaa tgggggactc
     1861 tggttaacac cctttgttcc ttcaaacgcc actatgacgc caacacaccg ctggcgcagg
     1921 tgatgccgga acttgttgaa caatatcctg acacttacgc gaacatgggg attcacgatc
     1981 tgggtgacac catgtttgcc tggctgaaag aaaacaacac tggcgcacgg ttgaacgaag
     2041 cctattccgg cctgccgatg gcggaaatca ccccgcgtga agcgtacaac gcgattgtcg
     2101 acaacaatgt cgaactggta tccattgaaa atctgccagg acgcatcgcg gcaaactcag
     2161 ttatcccgta tccgccagga atcccgatgc tgctgtctgg tgaaaacttc ggcgataaaa
     2221 acagtccgca agtaagttat ttacgctcgc tgcaatcctg ggaccaccat ttccctggat
     2281 ttgaacacga aactgaaggg actgaaatta ttgacggtat ttaccacgtt atgtgcgtga
     2341 aagcgtaacc actattccgc tgaaggcgta attgtttaaa taacattacg ccg
//
LOCUS       DAZGLJ010000371         2359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000371 DAZGLJ010000000
VERSION     DAZGLJ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.379"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..587)
                     /locus_tag="ACHK49_004915"
     CDS             complement(<1..587)
                     /locus_tag="ACHK49_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO9265377.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVRVEEMQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKN
                     NITQGSGKEWSKQDRDTFDPTHDLDLYCDQASGLV"
     gene            complement(645..992)
                     /locus_tag="ACHK49_004916"
     CDS             complement(645..992)
                     /locus_tag="ACHK49_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO9265378.1"
                     /translation="MVTKTITEQRVEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETALTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            complement(1029..>2359)
                     /locus_tag="ACHK49_004917"
     CDS             complement(1029..>2359)
                     /locus_tag="ACHK49_004917"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO9265379.1"
                     /translation="EYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTGY
                     DGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTAC
                     SAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGNQ
                     FEALPAEVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLAD
                     ELINASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLAT
                     MLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPEM
                     TVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLAA
                     GSGKAEPVSASTTAMFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
BASE COUNT          466 a          754 c          602 g          537 t
ORIGIN      
        1 ttcacaagac cgctggcctg gtcgcagtag aggtcaagat catgtgtagg atcgaacgtg
       61 tcacgatcct gttttgacca ctccttacca ctaccctgag tgatgttatt cttcgtcgac
      121 ctgccaaaat cgacctcaat tttctcgaac tggtctcctt ccatcgtgta tttgccatac
      181 agcacggcat tcaccgcctg catttcttcc acccggacaa tggcgtgctc ttcctgtttg
      241 aggttatcag tgatgatacg cagacgacgg taggccgggt cgttcagctg agccggatct
      301 tcaccgggaa gacgctcaac cgcctgctgg taattaaatt cgtgtttcgg cttgacgtag
      361 cccggacgta acacgcgggt ttcaccacca cgatgacgca gcacttttcc ttcaacaacc
      421 ggggaaacat aggccgccac cggcgttttt ccggtaattt tgtccagcat cacctcttcg
      481 gtatggaaat tcaccgtacg gcggaaaaac agctccagaa acagcgcacg aaatttcact
      541 ttttgttcgg tataaccgag taactggcgg gtcgtaaaca atcccataaa tcagttcctt
      601 tcattcagaa atcagtcagg ccaccgcggt ggcctgataa cgtgttacgg cagcgccgcg
      661 tgactcaggg cactgccggc aaaggcgttg gcctttttgt gttcatccac actttcaggc
      721 cagcggattg cctccgtcgc aaaggtcccc gacttgtaat aggtcagcgc cgtctctgtg
      781 ccttcaagcg gcagtaccag tatgccaacc gcactaccgg ctttctgtcc gtcccagacc
      841 accagtttcc cggtggcctc atccagcatc aggggcgtca gtgccggtgt tgccgaggaa
      901 atcccgctgc tgcctgtggc ggtatgagcc ggatcattac cggcaaaaat acgtacttcc
      961 acacgctgtt cagtgatagt tttcgtcacc atattgtaaa aacctcctgt tgatggtcag
     1021 cactggcttc atggcatggc catgagcatt ttcacgtccg catcaccgtc tgctgacgtc
     1081 tgtggcacgc caccctgtac cgctgccggt gaatggttcg ccatgatgcg ttcaaacatg
     1141 gcggttgtgg atgcagagac cggttctgcc ttacctgatc ccgcagccag cacagcccgg
     1201 gcgctctcca cagtcattcc cgggcaggca gccagctgtt cagcctgcgc ctcagcccct
     1261 tttgcctcat ccagtgccat gatctgatca cggagtgagg gtccggcatc cgcctgcggt
     1321 gaagcagcca ggatcgggcg ggctttttcc accgtcatct ccggcatcgc cgccagcgtt
     1381 gccgccagtt gttcacgacc tttcgcttct tcacacgcca taatgcgatc ggcttcactc
     1441 tgcgcggatg ccaccggctg ctgtggtgcc gccgcggcca gaatcgcccg ggcctgttca
     1501 acgctcatgc cctgttgtcc tgccagcatc gtggcaagct gttcacgtcc tttcgcttcc
     1561 tggcatgtca ggatccccat cactcgctgg ttctcctgcg cggcggcttc cgttgcagtt
     1621 aattgcggca tagtgcctcc tctgacatta ctgttcagcg ccgtggccat cacactgatg
     1681 gcatccgacg cattgattaa ttcatccgcc agcccggcct caatgccgga ctgaccttca
     1741 aaaacggcgg cctctgttcc cgtgacagca tcaacagaca gaccggtata catcgctact
     1801 ttttcagcaa acatccggcg cgccgcatca atgcgctgtt gcatgtcctg gcgaacctct
     1861 gccggcaacg cttcaaactg attgccatcc accttgtgcg cccctgagta aatcagcgtg
     1921 atatcaacac cggcctgcgc cagatgaccg gcatagctga catggctcat catcacgcca
     1981 atggagccga tacgggatgt ctgggtaacc agccgtcggg agcaggccga cgccagcagc
     2041 atggctgcag agcaggccgt gtcattgcac agtgcccaga ccggcttctg ctgacggagg
     2101 cggtaaatca tgtcagcgca gtcaaacgcg ccggcggcct gcccgcccgg actgtcaatg
     2161 tccagcagta cgccccgcac ctggctatcc gccattgcct gctgaagaca ggcgacaatg
     2221 ccgtcatagc ctgtcattcc ggaaaatggc cgcatacccc ccagccggtg caccagcgtg
     2281 ccggtcaccg gcagtacagc aataccgttc accacccggt aaacacgggc cggtcgttta
     2341 cctccggcca tgtactcgt
//
LOCUS       DAZGLJ010000372         2355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000372 DAZGLJ010000000
VERSION     DAZGLJ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.380"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            152..643
                     /locus_tag="ACHK49_004918"
     CDS             152..643
                     /locus_tag="ACHK49_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO9265380.1"
                     /translation="MSTETIEIFNNSDEWSNQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            978..2354
                     /gene="ygeH"
                     /locus_tag="ACHK49_004919"
     CDS             978..2354
                     /gene="ygeH"
                     /locus_tag="ACHK49_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFO9265381.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYDFTPESIYR
                     AMTIFDILQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDIT
                     TVDGKILAIMGLITGLSGQAKVSHILFEQAKIHPTDIASLYYYRALVHFHNEKIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLK
                     QLTRICMR"
BASE COUNT          875 a          392 c          387 g          701 t
ORIGIN      
        1 tttgtagtga acagtacttt taccaataat gaaaaatata ccataagcaa tgcaagctta
       61 aaactcataa tttaaaaaag ataaatataa aaatatcaat gagtcaatca ataataattt
      121 ggcatcacga gacacatcac agaggaatat tatgagcaca gaaacaattg aaatattcaa
      181 taatagtgat gaatggtcaa atcaactaaa acacgcatta tcgaaaggag aaaatctggc
      241 attactacat ggtttaactc ctgatatcct tgatagaata tatgcatatg cattcgacta
      301 ccatgaaaag ggtaatataa cggacgcaga aatttattat aaatttttgt gcatttatgc
      361 gttcgaaaat catgagtatc taaaagattt tgcatcagta tgtcagccca aaaagaaata
      421 tcaacaagca tatgaccttt acaaactaag ttacaattac tccccgtatg atgactattc
      481 agttatttat cgtatgggtc aatgtcagat tggggctaaa aatatcgata acgcaatgca
      541 atgtttctat cacattatta acaattgtga ggatgatagt gttaagagta aagcgcaggc
      601 atatattgaa ctcttaaacg ataattcaga agataatggc taagcattac aatcatttgg
      661 ttaaatttgc tggttatagt gcagttaacc actataacca gcacctctta tgttttctta
      721 aatcagcatc ttaaaacagt taagagcata ctataaatca ttatttttca atgcgttaga
      781 gaacaatcta ctttatcaaa attgaataag gctatttcag attacatcac tgaattccta
      841 agataaatta gtgaacacaa ccatagatat gtcgaatgta atttcctgta gcgttaatga
      901 cacataaata tgaatagcca taatttctat tgcttaagca ggatgcaaga aaccaatttt
      961 ttcatagagg ttaactaatg gacttagaaa ataaattctc atatcatttt cttgagggat
     1021 taacgctcac ggaagatgga attcttactc aaggaaatga gcaagtttat attccacaga
     1081 aagagttagg tgtattaata gtattacttg aatccgctgg tcatgttgta ctgaaagata
     1141 tgatcatcga atcagtatgg aaaaatatta ttgttagtga cgagtccctg acaagatgta
     1201 tctattcttt gcgctgcatt tttgaaaaaa ttggctatga tcgttgcata gaaacaatct
     1261 accggaaagg ttatcgtttc agcgggcagg ttttcaaaac taaaattaat gaagataata
     1321 cttcagacta ttccatagct atattccctt tcactacttc gttgaataca ctggatccat
     1381 taatacttaa tcaggaatta gtgcaaatca tttcaaataa aaaaatcgat ggtctctata
     1441 cctatccgat ggctgcgaca aatttttgta atgatcacat atctcaaaat tcattcttga
     1501 gcagattcaa gccagattat ttcgttacag gaagaataaa ccagaataat gcagtgaaca
     1561 ctttatacat tgagttgatc gacgctaaaa accttttcct catcgccagt aatcatctcc
     1621 ctgttgatga actacataat acatcacaat ttattataga taatatcctt caaacggtac
     1681 ataaaccaga acgatctgta agattagcta agcaggacca aggatataag aatcattatt
     1741 tatcagatga aatgttagcc ggaaagaaag aactttacga cttcacccct gaaagcattt
     1801 acagggccat gactatattt gatatattac aaaataaaag tgatatacag acgctaaaaa
     1861 cagaatgtta ttgccttcta gcggaatgcc atatgtcttt ggcacttcat ggaaaaagtg
     1921 aacttgaact tgctgctcaa aaagcattag agcttttaga ttatgtatca gacataacca
     1981 ctgtcgatgg aaaaatttta gctattatgg gactgataac tggtctgtct ggacaagcaa
     2041 aagtatctca tatcttattt gaacaggcta agatacaccc aactgatata gcctctctct
     2101 actactatag ggcactagtc cactttcata atgaaaaaat tgaagaggca aggatttgta
     2161 tagacaaatc actacaactc gaacccagaa gacgaaaagc agttgtgata aaagaatgtg
     2221 tagatatgta tgtgcctaac ccgctcaaaa acaacattaa actctactat aaagaaactg
     2281 agagtgaaag ccatcgagtt ataattgaca acattttgaa attaaagcag ctgacgagaa
     2341 tttgtatgcg ataaa
//
LOCUS       DAZGLJ010000373         2354 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000373 DAZGLJ010000000
VERSION     DAZGLJ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.381"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..537
                     /locus_tag="ACHK49_004920"
     CDS             <1..537
                     /locus_tag="ACHK49_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFO9265382.1"
                     /translation="CASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSI
                     YWEDPETAILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGYE
                     AQFGVQPTFVFLVASTTIECGRYPVEIFMMGEEAKLAGQLEYHRNLRTLADCLNTDEW
                     PAIKTLSLPRWAKEYAND"
     gene            530..1339
                     /gene="recT"
                     /locus_tag="ACHK49_004921"
     CDS             530..1339
                     /gene="recT"
                     /locus_tag="ACHK49_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFO9265383.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAIKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFNFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            1395..1544
                     /gene="ralR"
                     /locus_tag="ACHK49_004922"
     CDS             1395..1544
                     /gene="ralR"
                     /locus_tag="ACHK49_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFO9265384.1"
                     /translation="MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALER"
     gene            1582..1770
                     /locus_tag="ACHK49_004923"
     CDS             1582..1770
                     /locus_tag="ACHK49_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO9265385.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            1870..2085
                     /gene="xisR"
                     /locus_tag="ACHK49_004924"
     CDS             1870..2085
                     /gene="xisR"
                     /locus_tag="ACHK49_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFO9265386.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            2087..>2354
                     /locus_tag="ACHK49_004925"
     CDS             2087..>2354
                     /locus_tag="ACHK49_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFO9265387.1"
                     /translation="MSKLPTGVEIRGRYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDI"
BASE COUNT          746 a          483 c          555 g          570 t
ORIGIN      
        1 tgcgcaagca caggaaaaac ggttatcact gccgaagaag gccggaaaat tgaactcatg
       61 tatcagagcg ttatggcttt gccgctgggg caatggcttg ttgaaagcgc cggacacgct
      121 gaatcatcaa tttactggga agatcctgaa acagcaattt tgtgtcggtg ccgtccggac
      181 aaaattatcc ctgaatttca ctggatcatg gacgtgaaaa ctacggcgga tattcaacga
      241 ttcaaaaccg cttattacga ctaccgctat cacgttcagg atgcattcta cagtgacggt
      301 tatgaagcac agtttggagt gcagccaact ttcgtttttc tggttgccag cacaactatt
      361 gaatgcggac gttatccggt tgaaattttc atgatgggcg aagaagcaaa actggcaggt
      421 cagctggaat atcaccgcaa tctgcgaacc ctggctgact gcctcaatac cgatgaatgg
      481 ccagctatta agacgttatc actgccccgc tgggctaagg aatatgcaaa tgactaagca
      541 accaccaatc gcaaaagccg atctgcaaaa aactcaggga aaccgtgcac cagcagcaat
      601 taaaaataac gacgtgatta gttttattaa ccagccatca atgaaagagc aactggcagc
      661 agctcttcca cgccatatga cggctgaacg tatgatccgt atcgccacca cagaaattcg
      721 taaagttccg gcgttaggaa actgtgacac tatgagtttt gtcagtgcaa tcgtacagtg
      781 ttcacagctc ggacttgagc ccggtagcgc cctcggtcat gcatatttac tgccttttgg
      841 taataaaaac gaaaagagcg gtaaaaaaaa cgttcagcta atcattggct atcgcggcat
      901 gattgatctg gctcgccgtt caggtcaaat cgccagcctg tcagcccgtg ttgtccgtga
      961 aggtgacgag tttaatttcg aatttggcct tgatgaaaag ttaatacacc gcccaggaga
     1021 aaacgaagat gcccctgtta cccacgtcta tgctgtcgca agactgaaag acggaggtac
     1081 tcagtttgaa gttatgacgc gcaaacagat tgagctggtg cgcagccaga gtaaagctgg
     1141 taataacggg ccgtgggtaa ctcactggga agaaatggca aagaaaacgg ctattcgtcg
     1201 cctgttcaaa tatctgcccg tatcaattga gatccagcgt gcagtatcaa tggatgaaaa
     1261 ggaaccactg acaatcgatc ctgcagattc ctctgtatta accggggaat acagtgtaat
     1321 cgataattca gaggaataat tcagcctggc ggtgtaatgc accgccaact tgaaatattt
     1381 ttatgagaaa aattatgaga tatgacaatg ttaaaccatg tccattttgt ggttgtccat
     1441 cagtaacggt gaaagccatt tcaggatatt accgagcgaa gtgtaacgga tgcgaatccc
     1501 gaaccggtta tggtggaagt gaaaaagaag cactcgaacg atgaaataaa cgaaccactg
     1561 gaaataataa tggaggtgtt catgtataaa attaccgcca ctattgaaaa ggaaggtggc
     1621 actcctacta actggacaag atattcaaaa tctaaactaa cgaaatcaga atgcgaaaaa
     1681 atgctctcag gtaaaaaaga agcaggcgtt tccagagagc agaaagtaaa actgataaat
     1741 tttaattgcg agaaacttca gtcctcgtga attgcattgt attcaaatta aaacttcata
     1801 gctgattatt aataatcaac atcgggcgtc aatttcagtc taacattggc gcctgccaga
     1861 ggtgatgcga tggcacaagt aatctttaat gaagagtgga tggttgaata cggcctgatg
     1921 cttcgcactg gtctgggggc cagacaaatt gaagcatacc gccagaactg ttgggtggag
     1981 ggcttccact tcaaacgagt atctccttta ggtaagccag acagcaaacg agggattatc
     2041 tggtacaact atccaaagat aaatcagttt atcaaagact catgatatgt ctaaattacc
     2101 aacaggtgtc gagattagag gtagatacat tcgcatctgg ttcatgtttc gaggaaaacg
     2161 atgtcgggaa acattaaaag gctgggagat tacaaacagt aatattaaaa aggccggaaa
     2221 tttaagagcg ctgatagttc atgaaataaa ctccggtgaa tttgagtatt taagacgttt
     2281 tccccagtcc agcactgggg caaaaatggt gacaacgaga gtcataaaaa cgttcggaga
     2341 gctttgtgat atct
//
LOCUS       DAZGLJ010000374         2339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000374 DAZGLJ010000000
VERSION     DAZGLJ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.382"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1626)
                     /locus_tag="ACHK49_004926"
     CDS             complement(<1..1626)
                     /locus_tag="ACHK49_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9265388.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYVAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDAL
                     YLQQQNYSLEALSRRDAREDPFASAGKTVSSQLPDGASDGNKAISETEHDAVKAMFRG
                     DTEKMTERELSIIRALGEEFSTVLADLQRTFEGKMASQAQAFEEKLTSLSAVLQKHVT
                     VDEVRPVLQAMVDDAVGAIPVPRDGRDYDPDVLQ"
     gene            complement(1572..1793)
                     /locus_tag="ACHK49_004927"
     CDS             complement(1572..1793)
                     /locus_tag="ACHK49_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265389.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(1838..>2339)
                     /locus_tag="ACHK49_004928"
     CDS             complement(1838..>2339)
                     /locus_tag="ACHK49_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO9265390.1"
                     /translation="AVLTEELIRFSSPAADALVRNALAEAVVARLDTDFVDPKKAAVA
                     DVSPASITHDVKGTASTGNPDADAEAAFGQFVAANLQPTGAVWLMSSTNALALSMRKN
                     ALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAPDIYLADDGGVAVAVITGVNY
                     GSASGG"
BASE COUNT          476 a          766 c          573 g          524 t
ORIGIN      
        1 ctgcagtaca tccggatcat aatcacgacc atcacgcggt accggaatgg cccccacagc
       61 gtcatccacc atcgcctgca gaaccggacg cacctcatcc accgtcacat gcttctgtaa
      121 taccgccgac agggaagtca gtttctcttc aaacgcttgt gcctgcgagg ccatcttccc
      181 ctcaaatgtg cgctgtaaat ccgccagcac tgtggagaat tcttctccca gtgcacgaat
      241 aatggacagt tcccgttccg tcattttctc agtatccccc ctgaacatcg ctttcaccgc
      301 atcatgctct gtttcactga ttgccttatt accgtcagat gcgccgtcag gcagctgtga
      361 tgaaactgtt ttcccggcag acgcgaacgg atcctcacgg gcatcacgac gggacagcgc
      421 ctccagactg tagttctgct gctgaagata cagtgcatca ccgccggcca ggggcggcag
      481 gttctcccgt ttacgggcct cattgggcgt gagaagcgta tttttcaccg catcccccag
      541 cgttttcatg cgccgctcac tgtccattct cagcagcgtg gtgacatcaa attctgtact
      601 ctcgttttcc cccgtttcca gcgcctcatc cagtaacagt tcaatggact caatcagcgt
      661 ctgcaggcac tgggaataat actgctgctc cagcgcctcc acgttgtcac tggaaggcgg
      721 ttgtcccacg ccaatcttgt aggccgggac acggaacacc gaacagacaa tttcagcggt
      781 catcttcagt tgttccaccg tctgcgcatc cacaggtgaa aacgtcgtgg ggttgtattt
      841 tgccccgttg ctcagaatgg ccgttttccc cgcattttcg cctgtatacc cgctgtccca
      901 gttgctcttc agttttttcg cattttcttc cgtaatactg ccggggatct caatcacccc
      961 ggacggcctg ccgccatttc tgaaaaaaga cgtcgaattt tcctgaatat gatgcccctg
     1021 cgtggccgcc agcccggcga catacaccgg cggcaatcct ataagcggat gaaaaaaaca
     1081 gttaaaccgg tcgtggatca cttcccgggc aggcaccgtc accgcctccg tgatcccgca
     1141 gttccggtcc ggcgtgatgc ggtagaacac ctcgccgtca tccgccacca gaggttcaac
     1201 ccggctccag tccagaatac gcagttcttt gatctgcccc cgggcattac ggattttcag
     1261 caccaccgta ttgccatgac gcagtttggc gttcagccac agttcaaaaa actggatgcg
     1321 gttctgctgg gcgttgggac gacgacagag gcgggcaata tccccccggc gcgtttccct
     1381 gcgtatccca tgcgcatccg tctgcataag acgcagccgc attttggcga tatcctggga
     1441 tatcagcgaa atacatgcaa acaccgcatg aaaggagagg acggcttcag gatcggcttt
     1501 cacgccctgc tgccaggcgc cggaaaaggg ctcagccacc gcctgaaaca ggctggtcca
     1561 gcccgcctct cttacgtcac gtcctgattt ctggtttttt cgggttcgcc gtaaaaggtt
     1621 ccacattcgc catgctccgc atcacgtttc tttttctgac ctgccggacg tcgcaccgtg
     1681 atgtactccg cctttcccag gcgaaccagc acctccgcac acggctgtgc cacatcacgg
     1741 atatccccgg cccgggcatc atgcgtgccc tgcagatatc ggatctttgc cataacctgt
     1801 tacgggaggc gcacgcctcc cgtcctcctt atcagactca gccgccggac gcactgccgt
     1861 agttcactcc ggtgatcacc gccaccgcca caccgccgtc atcagccaga taaatatccg
     1921 gggcattcac cagcaccagc tggtcaccca cgtactggga gacaatcacc ggcagcccct
     1981 ggaaggagcc acccagcagg gtcatgtccg ggtattcctt ctgacccagc gcatttttac
     2041 gcatggacag tgccagggca ttggtgctgg acatcagcca gaccgcaccg gtgggctgca
     2101 ggtttgctgc cacaaactgt ccaaacgcag cctctgcatc cgcatccggg ttaccggttg
     2161 atgccgtgcc cttcacatca tgggtgatgg acgccgggga gacatctgcc actgcggctt
     2221 ttttcgggtc cacaaagtct gtatccagac gcgccaccac cgcttccgcc agcgcattac
     2281 ggaccagtgc atcagcagcc ggactggaaa aacggatcaa ttcttccgtc agtaccgca
//
LOCUS       DAZGLJ010000375         2320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000375 DAZGLJ010000000
VERSION     DAZGLJ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.383"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1164
                     /gene="iha"
                     /locus_tag="ACHK49_004929"
     CDS             <1..1164
                     /gene="iha"
                     /locus_tag="ACHK49_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO9265391.1"
                     /translation="ENKGRELVRSVLKRDKWGLAGQPRELKESNLILNSLLLTPLGES
                     HLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDEWHLTDALALTAGSRYEHHE
                     QFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLHKGISGVSGQGKTNLLGNPD
                     LKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSYSINDNTNSYVNSGKARLHG
                     VEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSYTPEHMVNAKLNWQITEEVA
                     SWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAWTVVDAGLSWKMTDALTLNA
                     AVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYVIPERNYWMSLNYQF"
     gene            complement(1382..1532)
                     /locus_tag="ACHK49_004930"
                     /pseudo
     CDS             complement(1382..1532)
                     /locus_tag="ACHK49_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1744..2064)
                     /locus_tag="ACHK49_004931"
                     /pseudo
     CDS             complement(1744..2064)
                     /locus_tag="ACHK49_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
BASE COUNT          608 a          528 c          597 g          587 t
ORIGIN      
        1 gaaaataaag gtcgtgagct tgtacgcagt gtactgaagc gcgacaaatg ggggcttgcc
       61 ggtcagccgc gggagcttaa ggaatcgaac cttatcctga attcattact gcttacccct
      121 ctgggagaat ctcatctggt tacggtgggg ggcgagtttc agagctcgtc catgaaagac
      181 ggagttgtcc ttgccagcac aggtgaaact ttccggcaga aaagctggtc ggtatttgct
      241 gaggatgagt ggcatctcac ggatgcactt gcgctgactg cgggcagccg ctatgaacat
      301 catgagcaat tcgggggaca cttcagtccg cgtgcatatc tggtctggga tgtggcagat
      361 gcctggacgc tgaaaggcgg tgtgaccacg ggatataagg cacccagaat ggggcagcta
      421 cataaaggga ttagtggtgt gtccgggcag ggaaaaacaa atctacttgg taaccccgac
      481 ctgaagccgg aagagagcgt cagttatgag gctggggtgt attacgataa ccccgccggt
      541 ctgaatgcca atgtcacagg ttttatgact gacttctcca acaagattgt ctcttattcc
      601 ataaatgata acaccaatag ctatgtaaac agcggaaagg cccggttgca cggtgtggaa
      661 tttgccggca cattgccgct gtggtcagag gatgtcacgc tgtcactgaa ttacacctgg
      721 acccgaagtg aacaacgtga tggtgataac aaaggtgcgc cgctgagtta tacccctgaa
      781 cacatggtga atgcgaaact gaactggcag atcaccgaag aggtggcatc atggctgggt
      841 gcccgttatc gcgggaaaac accacgtttc acccagaatt attcgtcact gagcgctgta
      901 cagaagaaag tgtatgatga gaaaggagaa tacctgaaag cctggacggt ggtggatgca
      961 ggtctgtcgt ggaagatgac ggatgccctg acgctgaatg ctgcggtgaa taacctgctc
     1021 aacaaggatt acagtgacgt gagcctgtac agtgccggta agagtacgct gtatgccggt
     1081 gattacttcc agacgggatc atcaacaaca ggatatgtga tacctgagcg aaattactgg
     1141 atgtcgctga actatcagtt ctgataataa caaaacgcta tcactgacgg tagaatacgt
     1201 tgccactgca actcctggcg gaacagtggc aacgtattag gttaagtgca tttccgatcc
     1261 gctaatgaga tttcgttacc aacaactaat atcgtcacag gaaatgcacg gattattttt
     1321 aacttatcat ttacatactt gtccagagtg taagcgcacc gtgaaggacg tggggtaaaa
     1381 attagtttac agagagagtg acgttccagg gcaacaactc tttcatgcgg ttggcaggcc
     1441 aggtgttgat tacactgatc acgtggcgta gccacgtttc cggctcgatt ccgttaagtt
     1501 ttgcagctac cgatcaggct gtacatcact gccgcactat cgctcgtcat ctcaaagtcc
     1561 ggtctcgtca gcaggaaggt atcattctct cccgccattt ttccaggggc ccggtcagat
     1621 aagtcccttt gtctatcgct gactcctgac tcataacccg gttagcagaa tgcagggtca
     1681 ccacctcgcc acgaccaaat ccaaataagt caattgcacc ttctcaatcg ccattttgtc
     1741 agtaagcgta cagcctgaac tgatggtatc ttcaccatca atgacaacgg tgatcgcaat
     1801 tttactgacg ttcgccggaa cacgatccag tgctatctca atgctggcct gctgcgaacc
     1861 ggtaacgagc ctgactgccc cctcaggaga agacaaatta ttataaaaga taaagtcaga
     1921 atcgccactg acctttccct gagcattaag catgaacagg caggtatcgg gttcgccttt
     1981 aaagccggat ttggctgggt actgaagatt cagcctgatc gtagattgct gaaggggtat
     2041 gttttgtccg gattgtaaat tcatattaac tctcctgatt tttgattatt attaatgcgc
     2101 agcgtttata tatgttccct aggcttagtt ctggacgctg gatattcggt gaggcgtaaa
     2161 tatggtatga caccattttt cataacgctg aagtttctat acctgttgaa tttgaatttt
     2221 cattgaccgg gtatcttatt ttccagggcc cgttccttca taagtcgcaa aagtaacata
     2281 tatccgaagg gcatgctgtt gatatcagac acggaatact
//
LOCUS       DAZGLJ010000376         2319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000376 DAZGLJ010000000
VERSION     DAZGLJ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.384"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1867)
                     /locus_tag="ACHK49_004932"
     CDS             complement(<1..1867)
                     /locus_tag="ACHK49_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9265392.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AVAREQRRALAELNSQLTEIRASAAGTAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGVLRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     VTKTLSGVLNGVAGNIDNVATAAGALVAVGVARYFGNMASGAVSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAART
                     AAQNELNSTTAVGSRLMTGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALAEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLSQATEQLAVEQSRLAQMQEKVQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQAT"
     gene            complement(1915..2124)
                     /locus_tag="ACHK49_004933"
     CDS             complement(1915..2124)
                     /locus_tag="ACHK49_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO9265393.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            complement(2220..>2319)
                     /locus_tag="ACHK49_004934"
     CDS             complement(2220..>2319)
                     /locus_tag="ACHK49_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO9265394.1"
                     /translation="REQVLAVYGPVHARLLRQALELIADAESARKK"
BASE COUNT          453 a          780 c          569 g          517 t
ORIGIN      
        1 gggtggcctg cggcagccgt aacggtacat tcaccagtcc ctgacgctgc tgaagcagtt
       61 cattccccag ccccagcaga cggttgaatt ccgtatgctg accgttcata accagcatgg
      121 actggtacac cttattctgc tctgccgcct gctgacgaat taacgccaca cgacggtctt
      181 ccagcccggc aagcacatcc tgaatggact gcactttttc ctgcatctgt gccagacggg
      241 actgctcaac ggcaagctgc tctgttgcct gagaaagccc ttccgtcacg gtcttcaccg
      301 atgtcagatg gtttatcatg aatccgtcac cggttgtcca gccagggtta gccagaacat
      361 actgatatcc agcgattttt tcctgcaggg atttcacccg gctggcctgt tcatcaatca
      421 gccggttctg ctctgccagc gccgcccgtg ttcgtccttc attatctgag gcttcaggca
      481 aagacattga cggcgtttta tgcgcgattt catctatcgt cagtgcatac tggcgcgctg
      541 actccctggc ctgctcctga ttctggtaca gcgtatacca tgctgctgcc cccagcatca
      601 ccagtccggg tacgccacca accagtccca acgcaccagt catcagacgt gagcccaccg
      661 ccgttgtact gttcagctca ttctgggctg cggttctggc agcaatattt ctgttcaggc
      721 gttcctgtgt ggccgccaga cgggcttctg cagcaatctg catctccgtc ccgcgggctg
      781 ccgccacagc ctgctgtgca cggtacacgg ctgcccttgc ccgcgccgtg gcaatctgcg
      841 tccccctgag ctgtgcttcc gccagtgcca cttcattacg tgctgccgtc acaagtcctg
      901 ccgtggcaga caccgctccg gaggccatat tgccaaagta ccgggcaacc ccgacggcaa
      961 ccagcgcccc cgcggctgtt gccacattat caatattacc ggcaacaccg ttcagcacgc
     1021 cggagagcgt ttttgtcacc ccgctggctt cattcgcgcc acccacccag gccataaagg
     1081 cgttttccac ctttgtgatc ccgtcagaga ccgtttccgg catggcggca tattcatcac
     1141 gcaatacccc cagctggctg attaacgcag gaacgacttt atccgccgtc agtttgccgt
     1201 cgtccgccat cgccttaagg tctttacggg ccacgcccat acccgcagcc agtgcacgta
     1261 cgatccggtc tccgctttca ttgaccgaat taaattcctc accgcgtaac acaccctgtg
     1321 ccagcgcctg gctgaactgg gtgatcaccg agcccgcctc tgccgtactg gcaccggaga
     1381 ttttcagccc cgtggaaatg gcctccgtca ccttcagcac atcatcagca ctgtaaccat
     1441 attcacgcat tgaggctgcc gagcgggcaa acagggccgc attatctgaa aatgccgtgc
     1501 ccgtccgctg actgatatcc atcagcactt tctgtgatga cgaaaattca tcggatgact
     1561 gcgacgcctg tttcagtcgg gcattcacgg aactccattc atcggccaga gaaatcaggt
     1621 gtccggtggc aaaggcacct gcaaatgccc ccgccgttcc ggcagctgaa gcgcggattt
     1681 ccgtcaactg gctgttcagc tcagccaggg cgcgtcgctg ctcccgggcg actgcggcag
     1741 cctgacgccc gccattctgc agggtccggt aatattcact gcccatgcgg gaagcccgct
     1801 ggatctccga ctggaatgac tgcgaatttg ccgaaatttt gataatcagt tcacgtaacg
     1861 tcgccattca cctttctccg ggcgtaaaaa aaccgcctca gcggttctca tcattcatga
     1921 ctgtgctgcg aagctcagcg cgtcttccag cgccgcaaac ggatccacct ccggcttatc
     1981 ctcatcctcg ccccagcaga gcatggcgtc cttcagtgca acattcatcc cctgtgcccc
     2041 aaaaaccgct ttcacgatct gtgcattacg gatatccccg cgctcatcac ccagcgggga
     2101 taccctgtcg aactccatcc acatcatcgc ctcgctcaca ctcaggctgt gccgcagttc
     2161 ggataaggtg cgccccagac ggagcgcaag tcgcatcaga aagcgaattt ccgggcgggc
     2221 tacttttttc tggccgactc tgcatcagcg atcagttcca gtgcctgacg cagcaaccgg
     2281 gcatgtaccg gaccatagac ggccagcacc tgctcacgg
//
LOCUS       DAZGLJ010000377         2303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000377 DAZGLJ010000000
VERSION     DAZGLJ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.385"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            94..594
                     /locus_tag="ACHK49_004935"
                     /pseudo
     CDS             94..594
                     /locus_tag="ACHK49_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            596..889
                     /locus_tag="ACHK49_004936"
     CDS             596..889
                     /locus_tag="ACHK49_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFO9265395.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
     gene            complement(950..2070)
                     /locus_tag="ACHK49_004937"
                     /pseudo
     CDS             complement(950..2070)
                     /locus_tag="ACHK49_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(2079..>2303)
                     /locus_tag="ACHK49_004938"
     CDS             complement(2079..>2303)
                     /locus_tag="ACHK49_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO9265396.1"
                     /translation="IVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTVKSALAFL
                     ILIIYFAPILAERVMPLSFFPEQLQLYIEK"
BASE COUNT          769 a          427 c          370 g          737 t
ORIGIN      
        1 ttccctcaat gttaagcaaa tataacaatt actcttattt taaattttac acgtaatatt
       61 taccctaaat gattttagag tacaggtggg catatgcaag tcttttccag tgatgtctac
      121 ttcactgtag gcactaatgc attattagca tcccaaaaag aatattacag cgatttagtt
      181 gcgttggttg atcttggaca ttcgttcgtt gttattgatg agcatcaaca tcgaaatctc
      241 aaaccaaata cagaacctgt aaacatcctt ttgagcaaca atttcatcag gatcaataag
      301 aatattacat tatctgattt aacgcatttc ctggtttcaa atcttcatac acaaaatgtg
      361 tattccacac aagaaccact tacccatgat gagatagata tcctgcggtt atgtgtttct
      421 tactcgttaa aacaaatagc cattattaaa ggaattgatt ataaaacaat atcatatcac
      481 aaaataagag ccctaaataa acttaacata aaaggtactg tttagttgtt tatagcactc
      541 tgtgaatggg ataaacatta ttttaaactc caatcttgcg taagagaaag ttgatatgtc
      601 attagtagga tataatagta acatcataat aaccatcgcg ctgatatcgt tgctctctgc
      661 atgcaccaga gaaaattcat taaaggagaa tcaaaataaa agaagtggcg aatcccaagc
      721 gatagtagat gcaagacagt tctttagcga acacccggaa tatatatcat cagcagaact
      781 ggagcaaaag ctatatcgcg aatttgcaca ggttactaca gttccagctt ataaagagat
      841 gagtatgtat caattgctta tcatttcaca agatcgttta actcattaaa taaggatcta
      901 cacgcttttt gcgtgtagat tcttataaat ttctgactac gacagatgtt taatttttta
      961 aattatcgtc tttattctcg atttttgtgt cttcaccctc aatgaattta tcttctgaag
     1021 agagctgttc atcttgaaca ggttgcccag cattctccac atcttcaagc cacagcagaa
     1081 gacgtaatat ttcatctata ttttcgaagc taacataatc gtagcgacga tgggtagcat
     1141 aattttttcg tgctaatttt ttatctgtga taattggtat tccgatttcc tttgcatatt
     1201 tccttacgcc taaggccacc tcattagtta ctcttacaga aatcaatgga ataggtgaaa
     1261 gatgtggctt aaagtatatc cctattgcaa tgtgagtagg gttggaaatc atcaaacgac
     1321 tattactgac atcagacttc aattgctcag aaagaatttc ctgatgcgct ctctgcgttt
     1381 agacttaatt tctggatttc cttcttgctc cttgtattct cttttaactt cttgtttatc
     1441 cattttcata tctttcataa ataaaaaata ttcagcgata aaatcaaaaa ttaagacaat
     1501 tatcatactg ccgagacaat acagtatgag aaggaatagc atctctcccc aatctgataa
     1561 taaagatatg atatctccat caagagtttt aaaaagcagt gatttatgat tactccaaaa
     1621 tactttgatc gccaatgcaa aaaaaacaat ataaagaatt gctttaacaa attcttttac
     1681 ggtttttaag ccaaatatac gctttaagcc atttactgga ttcaatgaat caaacttaaa
     1741 tttaacagct tcagttgcta attgcatttt actttgaaac caggagacaa gtattgttgt
     1801 gaacatgcaa accagcaaaa aaggaatgtt cgttttcaac caaccaaccc ccatagcttt
     1861 aaaatagtcc caaatatctg gtttagcgtt atgatcaagg atgtattcaa gaatcgacat
     1921 aatgtgatgc acatcaaaaa catatccgag atataaagta cccacaagca ttattacaga
     1981 gatggttaag tctctacttt atagaaattg ccctttttta gaagcatcct ggagtttctt
     2041 ttgtgttggc ttttcagttt tattggccat gaaacactct atttctcaat gtatagttgc
     2101 aattgttctg ggaaaaagga taacggcatt actcgttcag ccagtattgg ggcaaaataa
     2161 ataattaata ttaaaaatgc cagcgcactt ttgacagtca gtgaaatggc aaaagcattt
     2221 agttgtgaag cataacgtgc cagcaatccc aggacagcct caccgcccag cataacggca
     2281 atcacggggc ttgcgtaaac gat
//
LOCUS       DAZGLJ010000378         2302 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000378 DAZGLJ010000000
VERSION     DAZGLJ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.386"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..200
                     /locus_tag="ACHK49_004939"
     CDS             <1..200
                     /locus_tag="ACHK49_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265397.1"
                     /translation="SEINRNSISKKYKPIPLEAIEAKGDSAFVYKMLKGFARVNDGKV
                     RVSNSMVNGLEIVDCLIYELK"
     gene            324..425
                     /locus_tag="ACHK49_004940"
     CDS             324..425
                     /locus_tag="ACHK49_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265398.1"
                     /translation="MRTYNLNYLLPSQMQKCTCDFLYPAFDLCGGEA"
     gene            422..877
                     /gene="ybcN"
                     /locus_tag="ACHK49_004941"
     CDS             422..877
                     /gene="ybcN"
                     /locus_tag="ACHK49_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO9265399.1"
                     /translation="MNLPQDGIKLHRGNFTAIGQQLQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLIRRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            877..1047
                     /locus_tag="ACHK49_004942"
     CDS             877..1047
                     /locus_tag="ACHK49_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO9265400.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            1040..1330
                     /locus_tag="ACHK49_004943"
     CDS             1040..1330
                     /locus_tag="ACHK49_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO9265401.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            1327..1689
                     /gene="rusA"
                     /locus_tag="ACHK49_004944"
     CDS             1327..1689
                     /gene="rusA"
                     /locus_tag="ACHK49_004944"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO9265402.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1686..1826
                     /locus_tag="ACHK49_004945"
     CDS             1686..1826
                     /locus_tag="ACHK49_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO9265403.1"
                     /translation="MMFEFYMAERLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            1912..2295
                     /gene="quuD"
                     /locus_tag="ACHK49_004946"
     CDS             1912..2295
                     /gene="quuD"
                     /locus_tag="ACHK49_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO9265404.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
BASE COUNT          621 a          503 c          582 g          596 t
ORIGIN      
        1 tcagtgagat aaatagaaat agcatatcaa aaaagtataa gccgatacca ttagaagcaa
       61 ttgaggctaa gggggattca gcttttgttt ataaaatgtt aaaaggattt gctagagtca
      121 acgatggtaa agttcgcgtt tctaattcta tggtaaatgg tttagaaatc gttgactgtc
      181 tgatctatga gttaaaataa gaaatctctt gctgggtatc attattgttt aaatgacttt
      241 tgattttcaa taatcaactt gtcataatta agtcaccgga gtttgaactc ctccggtgac
      301 ttctgcgcta aacggggacg tttatgcgca catacaatct aaactatctt ctcccgtcac
      361 agatgcagaa atgcacctgc gattttcttt atccagcgtt tgacctctgc ggaggtgaag
      421 cgtgaacctc ccacaagacg gcatcaaact gcatcgcggt aacttcaccg ctatcggtca
      481 gcagctccag ccttatttgg aagacggcaa gtgctttcgc atggtgctta aaccgtggcg
      541 cgagagacgc agtctttccc agaatgcact tagccacatg tggtacagcg aaatcagtga
      601 ataccttatc cgcaggggga aatcgttcgc taccgcagca tgggtaaaag atgctctcaa
      661 acacacatac ctcggttatg aaaccaagga cctggttgat gtcgtaaccg gcgaaatcac
      721 tactatccag tcgttacgcc atacctccga tcttgatacc ggagagatgt atgtcttcct
      781 gtgtaaggtt gaagcctggg cgatgaatat tggctgccac ctgactattc cgcagagctg
      841 cgagttccag ctgctgcgcg acaagcagga ggcgtaatgg ctacaccgct tattcgtgtc
      901 atgaacggac acatctacag agtaccaaat cgtcgtaagc gtaagcctga gctaaaacca
      961 tccgaaatac caacactgct cggatatacc gccagcctgg ttgataaaaa atggttgcga
     1021 ctggcagcaa ggaggaatca tggctgattt gagaaaagca gcgcgtggtc gcgaatgcca
     1081 ggtaagaatc cctggcgtat gcaatggcaa ccctgaaacg tctgtactgg cacatatccg
     1141 gctggctgga ctgtgtggca ccggtattaa accgccagac cttattgcca ccattgcctg
     1201 ttctgcctgt cacgacgaaa tcgaccgccg cacgcatttt gtcgatgctg agtacgcaaa
     1261 agaatgcgcg ctggaaggta tggcgagaac gcaggttatc tggctgaaag agggggtaat
     1321 caaggcgtga atacctacag catcacatta ccctggcctc cgagcaataa tcgctattac
     1381 cgccataatc gcgggcgcac gcacgtcagc gcagaggggc aggcataccg cgataacgtc
     1441 gcccgaatca ttaaaaacgc aatgctggat atcggcttgg ctatgcctgt gaaaatccgc
     1501 attgagtgcc acatgccgga tcgccgtcgc cgtgacctgg ataatctgca aaaagccgct
     1561 tttgacgcac tcactaaagc aggtttctgg ctggatgatg ctcaggtcgt tgattaccgc
     1621 gttgtgaaga tgcctgttac caaaggtggg aggctggaac tgaccatcac cgaaatgggg
     1681 aatgaatgat gtttgagttt tatatggcag aacgtcttcg ccaccgctgg gggcgtctgc
     1741 gcttatatcg tttccccggt tctgttttga ccgattaccg aatactgaag aattacgcca
     1801 aaaccctgac aggagcagga gtatgaagtc agagataaca atcaactaat actgtttcgt
     1861 tgatttttgc ttgtaattgg cgttctggtc tgatttttgt ggagtaagtt gatgcgtgat
     1921 attcagatgg ttcttgagcg ttggggagcg tgggcggcta ataatcatga agatgtgacc
     1981 tggtcgtcca ttgccgccgg ttttaaggga ttaattactt caaaagtaaa atctcgcccg
     2041 caatgttgtg acgatgacgc gatgatcatt tgcgggtgca tggcccgtct gaaaaagaac
     2101 aacagcgatt tgcacgattt attagtagat tattatgtag tcggtatgac attcatgtca
     2161 ctggcaggta agcattgctg ctctgatggt tatatcggga aaaggttaca gaaggctgag
     2221 ggcataattg aagggatgtt aatggcatta gatatccggt tagagatgga tatcgttgtt
     2281 aataactcta attaatatgc ca
//
LOCUS       DAZGLJ010000379         2294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000379 DAZGLJ010000000
VERSION     DAZGLJ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.387"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..121
                     /locus_tag="ACHK49_004947"
     CDS             <1..121
                     /locus_tag="ACHK49_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFO9265405.1"
                     /translation="PSQWVAIGVLGSLLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            121..618
                     /gene="rrrD"
                     /locus_tag="ACHK49_004948"
     CDS             121..618
                     /gene="rrrD"
                     /locus_tag="ACHK49_004948"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO9265406.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYINVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            615..1052
                     /locus_tag="ACHK49_004949"
     CDS             615..1052
                     /locus_tag="ACHK49_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO9265407.1"
                     /translation="MSRVTVIISALVICIIACQSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGLTSDAELNYYRLRGGIDKITAQVNYLQEYIRTQCLK"
     gene            1202..1819
                     /locus_tag="ACHK49_004950"
     CDS             1202..1819
                     /locus_tag="ACHK49_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO9265408.1"
                     /translation="MLASLGGLVSCAAKVHFKEAGNAMNELIANHDFDFRQLVTAAEG
                     QPVTDTFQIARAFGKRHQHVIRAIKCLRCSEEFSTTHFWAVEKINDLGIFDKKQIYYR
                     MDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAELRKYSESYEAERNAVMLEYMKEK
                     DVASMSGRLLNRWGRTKKPQLLAKLERLERQGQFLLPGFDKGIQA"
     gene            complement(2007..2201)
                     /gene="ylcI"
                     /locus_tag="ACHK49_004951"
     CDS             complement(2007..2201)
                     /gene="ylcI"
                     /locus_tag="ACHK49_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO9265409.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
BASE COUNT          602 a          513 c          595 g          584 t
ORIGIN      
        1 tccgtcccag tgggtggcaa taggtgtgct gggaagtctg ctgtttggcc tgctgacgta
       61 tctgacaaat ctttatttca agattaaaga agataagcgt aaggctgcga gaggtgaata
      121 atgtcgccgt cattacgcaa ggctgttgca gctgctattg gtggtggggc tgttgccata
      181 gcgtctgtgc tcatcactgg tccaggtggt aacgatggtc tggaaggtgt cagctacata
      241 ccatacaaag atatcgttgg cgtatggact gtatgtcacg gacacaccgg aaaagacatc
      301 atgcccggta aaacgtatac cgaagcagaa tgcaaagccc tcctgaataa agaccttgcc
      361 acggtcgcca gacaaattaa cccgtacatc aacgtcgata taccggaaac aacgcgcggc
      421 gctctttact cgttcgttta caacgtgggc gctggcaatt tcagaacatc gacgcttctt
      481 cgcaaaataa accagggcga tatcaaaggc gcatgtgatc agctacggcg ctggacatac
      541 gctggcggta agcaatggaa agggctgatg actcgccgcg agattgagcg tgaagtctgt
      601 ttgtgggggc aacaatgagc agggtaaccg ttattatctc cgctctggtt atctgcatta
      661 tcgcctgcca gtcatgggct gttaatcatt accgtgataa cgccatcgcc tacaaagagc
      721 agcgcgataa agccacatcc atcatcgctg atatgcagaa gcggcaacgt gatgtagcag
      781 aacttgacgc cagatacaca aaggagcttg ctgatgctaa tgcgactatc gaaagtctcc
      841 gtgctgatgt ttctgctggg cgtaagcgcc tgcaagtctc cgccacctgt gcaaagtcaa
      901 cgaccggagc cagcagcatg ggcgatggag aaagcccagg acttacatca gatgctgaac
      961 tcaattatta ccgtctccga ggtggaatcg acaagataac cgcgcaggtt aactacctgc
     1021 aggaatacat caggacgcag tgcttaaaat aattttaatt tcactgaaat ttaacaagtg
     1081 actttcagga aaatgcctcg cagatgcggg gcatttttgt accggtattt caccgcgcac
     1141 cgcagcgcac aataaacacc gaacctgacc ctttggaatg ggcctttgag gataccagtt
     1201 agtgctggcg agcctcggtg ggctggtttc ctgtgcggca aaggttcatt tcaaagaagc
     1261 aggcaacgcc atgaatgaat taattgcgaa tcatgacttc gactttcgcc agttagttac
     1321 cgcagcagaa ggtcaaccgg taactgacac cttccagatt gccagggcat ttggtaaacg
     1381 ccatcagcat gtgattaggg ctattaaatg tttgagatgt tctgaggaat tctcgacaac
     1441 ccatttttgg gccgtcgaga aaatcaatga cttaggtatt tttgacaaga aacagattta
     1501 ctaccgcatg gactttagtg gcttcgttat gctggttatg ggatttaacg gggcaaaagc
     1561 cgatgctgtt aaagaagcct atatcaatgc gtttaactgg atgtcagcag aactccgtaa
     1621 gtacagcgaa agttatgaag cagaacgtaa cgccgtaatg ctggagtaca tgaaagagaa
     1681 ggatgtcgcc agcatgtcag gccgtctgct caatcgctgg gggagaacga aaaaacctca
     1741 attgcttgca aagctggaac gtctggagag acagggacag tttttattac cgggattcga
     1801 taaaggtatt caagcctgac acattatgcg ctgtatcgtc gccgtattcc cgcattaacc
     1861 atgaccgtag cccgacgggg aattccttct gcgtgagtgt gcgggaataa tcaaaaacga
     1921 tgcacaccgg gttttactgt gctgacagac gcagggttac cctcatagtc gcttttccgg
     1981 tgcgatggtg gaagaaaccg ggatgttcat ccatcatcac tttggattga tgtatatgct
     2041 ctcttttctg acgttagtct ccgacggcag gcttcaatga cccaggctga gaaattccca
     2101 gacccttttt gctcaagagc gatgttaatt tgttcaatca tttggttagg aaagcggatg
     2161 ttgcgggttg ttgttctgcg ggttctgttc ttcgttgaca tgaggttgcc ctgtattcag
     2221 tgtcgctgat ttgtattgtc tgaagttgtt tttacgttaa gttgatgcag atcaattaat
     2281 acgatacctg cgtc
//
LOCUS       DAZGLJ010000380         2293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000380 DAZGLJ010000000
VERSION     DAZGLJ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.388"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..315
                     /locus_tag="ACHK49_004952"
                     /pseudo
     CDS             1..315
                     /locus_tag="ACHK49_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="icd-like protein"
     gene            complement(321..626)
                     /locus_tag="ACHK49_004953"
     CDS             complement(321..626)
                     /locus_tag="ACHK49_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO9265410.1"
                     /translation="MATGSKNAKSQSLTARIPHDVIEGMESVKLDGESNAGFIVTAMR
                     GEIARRQAEGSGENLLISSLNALAQVEKLGIKAAEEIGQLVTVAREELQRRKTKEPE"
     gene            823..1023
                     /locus_tag="ACHK49_004954"
     CDS             823..1023
                     /locus_tag="ACHK49_004954"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO9265411.1"
                     /translation="MRNHTTHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RLQSPAGCVMVFAARIRQGVGL"
     gene            1020..1250
                     /locus_tag="ACHK49_004955"
     CDS             1020..1250
                     /locus_tag="ACHK49_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265412.1"
                     /translation="MSQEITLQQAVERAHQIEVICALAEDYPGMMTDSESGAIIGLLK
                     RLSGEVCVFLIDEQERRTLISNEKKCGGVHVQ"
     gene            1240..1689
                     /locus_tag="ACHK49_004956"
     CDS             1240..1689
                     /locus_tag="ACHK49_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265413.1"
                     /translation="MCNKTTPDAAAAALTTLMHALIDIECTAELAQKEEREEYTLFAL
                     ECIRYTATRSLNDAKNILVADCENGGGVMRDDRFNSLKQEFSGVPDDAADALSSMPEL
                     IRAAFFLLSTREYKSTGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            1682..1915
                     /locus_tag="ACHK49_004957"
     CDS             1682..1915
                     /locus_tag="ACHK49_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265414.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLDPVIMELQEIDILHDRYKEQHTGE"
     gene            1921..2220
                     /locus_tag="ACHK49_004958"
     CDS             1921..2220
                     /locus_tag="ACHK49_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9265415.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVTIKGVEERRITY
                     RREGYEYDCVMPVYQFRRDFSLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
BASE COUNT          643 a          541 c          550 g          559 t
ORIGIN      
        1 gaacagataa cccgctgcgc cgtttgtgtg tgtgatccca aacataagca cggggatgat
       61 agccgctatc aggctggtgg gcaatgcaat cagtctggtt cagttcgttg ccatacctgc
      121 aatgagcgct tttccctgta ctctttaagg aattgctcaa gggcaaaagc acatggcgcg
      181 aatctttctg attcatgctc tatctttctg cgccgtcttt tccgtgccgg tgataatgtt
      241 ttggtcaatt ctttatcggt cattgtgttg tcctgcatag caatgcgccg taatacctta
      301 caccacggcg ctgatggtga ttactctggt tctttagtct tgcggcgctg gagttcttca
      361 cgtgcgacag tgacgagctg cccgatctcc tcggcggctt tgattccgag tttttccacc
      421 tgcgccagtg cattgagcga agaaatcagg aggttttctc cgcttccttc tgcctggcgg
      481 cgggcgattt caccgcgcat ggcggttact atgaatccgg cgttgctttc accgtccagt
      541 tttacggatt ccatgccttc aataacatca tgcgggatcc gagctgtcag tgattgtgat
      601 tttgcgtttt ttgaacctgt agccatctgt aatcctctca atgaaagtgt aagacaatat
      661 acacataaaa agtcttacat aaaagcattg acatgtaagc cacctataaa taaagttact
      721 tacaccttgt taatgcaagg tgcagaaacg acgaaacccc gcactgtagg agcagtaacg
      781 gggcttctaa ccaccaacga taacgagagt atcgaggtag ctatgagaaa tcataccaca
      841 cacccgcaag ggcgggactc gcacaacctg aataaataca tttggcgttt tatcgccctg
      901 agcactgcac aaccgcgcgt gattaccatt gaggccacca gcgaacagga agcacgcctg
      961 caatccccgg ctggctgcgt gatggtattc gctgcccgta ttcgtcaggg ggtaggctta
     1021 tgagccagga aatcacacta caacaggcag tagaacgcgc ccaccaaatc gaagttattt
     1081 gcgcactggc agaggattac ccaggcatga tgaccgacag cgaatcaggg gcaatcatcg
     1141 gcttacttaa acgccttagc ggtgaggtct gcgtattcct gatcgatgaa caggaaagaa
     1201 gaacgcttat ttctaacgaa aaaaaatgcg gaggggtaca tgtgcaataa aaccacaccg
     1261 gacgcagccg ccgccgcact cactacgctg atgcacgcgc ttattgatat tgaatgcaca
     1321 gcagagcttg cacaaaagga agaaagggaa gaatacacac tattcgccct ggaatgtatc
     1381 cgatacaccg caacgcggtc gctgaatgac gctaaaaata ttcttgttgc tgattgtgaa
     1441 aatggggggg gggttatgcg tgatgatcgt tttaattccc tgaaacagga attttccggc
     1501 gttcctgatg atgcggctga tgcgctttcg tcaatgccag aacttattag agcggctttt
     1561 ttcttacttt ccacgagaga atataaatca acggggcttg atgtactgaa tatcgccgcc
     1621 gattatgcgg aatatgtggc agaggcgcgt tatagaagaa aatttcctga ggatgtaagc
     1681 catgcgtgat atttaccacc aaacaataga ccgcgcattt cttgcacttt ctcacagtga
     1741 aaacatgctg gaaatattgc gcatatggct tgaaacactt ggcgacaatg aacgcgacaa
     1801 acaaaaatca agaattgcca cggcattaat aacgcttctt gatcctgtaa taatggaact
     1861 gcaagaaata gatatattgc acgacagata taaagaacag cacaccggag aataaaaata
     1921 atgaaactta aatattctgg cttaactgcc agtggcaaca ctcaccctaa atttacgcgc
     1981 ggcgatattt accgcgacca gtacggcggc accgtaacga ttaaaggtgt agaagagcga
     2041 cgcattacct accgccgtga aggctacgaa tatgactgcg tgatgcctgt ttatcagttc
     2101 cggcgtgatt tttctctggt acaggcagcg ccccgcagta aaccaaccag cagggagaaa
     2161 gcacgcgcca atattcagga aataaaaaag atgcttaacg tattcagggg caagaaatga
     2221 aaagcgcacc gaacttaaaa aaacagcctt acgacaagat gaccgaagtc atcatttttg
     2281 cgggtagtga tgc
//
LOCUS       DAZGLJ010000381         2288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000381 DAZGLJ010000000
VERSION     DAZGLJ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.389"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..66)
                     /locus_tag="ACHK49_004959"
     CDS             complement(<1..66)
                     /locus_tag="ACHK49_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9265416.1"
                     /translation="MKHTELRAAVLDALEKHDTGAT"
     gene            complement(63..641)
                     /locus_tag="ACHK49_004960"
     CDS             complement(63..641)
                     /locus_tag="ACHK49_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9265417.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSVSQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(632..1006)
                     /locus_tag="ACHK49_004961"
     CDS             complement(632..1006)
                     /locus_tag="ACHK49_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO9265418.1"
                     /translation="MADFDNLFDAAIACADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRRWKGDVWP"
     gene            complement(1018..1413)
                     /locus_tag="ACHK49_004962"
     CDS             complement(1018..1413)
                     /locus_tag="ACHK49_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO9265419.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(1455..>2288)
                     /locus_tag="ACHK49_004963"
     CDS             complement(1455..>2288)
                     /locus_tag="ACHK49_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO9265420.1"
                     /translation="SRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLADPAYRR
                     RRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVANNITQSG
                     GTEWSKRDKSTYDLTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKLDTRRGS
                     HSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGNTQARGL
                     RTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDAFVSVQL
                     A"
BASE COUNT          458 a          723 c          589 g          518 t
ORIGIN      
        1 cgtcgccccg gtgtcatgct tctccagtgc atccagtacg gctgcacgga gttcagtatg
       61 tttcatcgct ttattaccat cctcagttga tgctgcagcg catagcccag ctctttcgga
      121 agacgttcac gccgtatccg ctcaatattt tgtttaaacg ccgtggtcag cggcaccgcc
      181 atcgggattt tcaccacatc aatggggtaa cggtttttcc cggccacacg ctgcataaca
      241 tgccaccggc catttttcag ttgctgaata aacgcgccgg gaatacgacg gttacccacc
      301 acaagcacgc tgccgccacc tttcagggat gaacgctgcc cctttttacg acgcctgcgg
      361 cgcgaaagga caacccgcgc attacccagc ttgattacgg gcaaatcccc ccggttaacc
      421 ttgattctgg cctgcggatt tttgaccgtg gcccttttca gcctggccct ttcctttacc
      481 agtttccggc gtacctttgt ctcacgggca acctgtgaca ccgactgcga tatcgcggat
      541 gaagcaacgc ggttaatggc cattgcggcg gcaccgggca ccgccgttct gctgatacgg
      601 ctgaggtttt caacggcctg ctcaagacct tttatggcca tacatccccc ttccagcggc
      661 gacggttaac ggcaggcggt acgccccgcc caagccagag atgacagctt ccgccatcat
      721 ccggcgaaat ccggtctatc cagaagtttt cctcaccgat ggtcagcgtg tcgccgcgcc
      781 gcagctgccg cacatcatca gtccggacaa acagggacgg gctggagcct tcaacgcgca
      841 cgccctgtcc ggcatagctg atattttcag ggtcatcaaa aacaccacgt atcacagcac
      901 cggactgctc accggatgtc atggtggctg acgttcccat gtacccgcgt atcgtttcat
      961 cggcgcaggc aatggcagca tcgaacaggt tatcgaaatc agccacagcg cctcccgtta
     1021 ttgcattctg gccaggccgc gctctgtcat ttcagctgcc acaccggcag agacacggaa
     1081 cgccgttccc ggcagcacaa atgccacagc ctcatcccgc gtggcgtgaa gtgcatcagt
     1141 atgcagcgtc accagtgcca caaccgtgac cagatcagcc gtatcagtca cggtatccgg
     1201 ctgcgctgat acaacctcat tttcatgtcc ggtcagcgca ttttccgggc tgacagatgt
     1261 gtcctgaccg gcagcgtcat ccgtgtcatc aagctcctct tccagctctg ccacacggag
     1321 tgccagttct tctttcgtcc ccgtcaggct gacatcacgg ttcagttgct cacccagcac
     1381 ctgaagacgg gcaatcagtt catctttcgt catggactcc tccacagaga gaaaatggcc
     1441 ccgaagggcc atgattacgc cagttgtacg gacacgaacg catcagggtc agccagcagc
     1501 atcagcggtg ctgactgaat catggtgaac tcacgcgccg gatcgccggt ggtcacccag
     1561 tttttcgggt agcgggcaga ggcgttaata ccttcgcgct gtgcgtccgc atcctgaatg
     1621 cagccatagg tgcgcagacc gcgtgcctga gtgttcccca gcaccatcgt gttgtccgga
     1681 aggaagttct ttttgacgtc gttttccacg tactgtccgg aatacacgac gatcgccgta
     1741 tcgccataca tccccttata ggacaccgct tcgcccaggt cttttaccgc tgtctccagc
     1801 tcggaatgag agccgcgacg ggtatccagc ttctccttga cggctttgaa ggaacggaac
     1861 agcgcccagc ctttcggatc aaacacgatg atattcacca caccgctggc gttcagcgcg
     1921 taggcttcga tatcgtcggt caggtcatac gtggacttgt cacgcttgct ccactccgta
     1981 ccgccggact gcgtgatgtt gttcgccaca ctgcggccca tatccacttc aaccggatcg
     2041 aaggcttcac cggtcatggt gtatttgccc ttaagcacgg cagaaactgc ctgcatctct
     2101 tcgacctgag caatggccag ctcttcgtct cgcatgttct gcaggatgat gcgacggcgg
     2161 cggtaagccg ggtccgccag attctgtgga tcttcatccg gcaggcgacg cagggtcatc
     2221 tgcggattca cttcatgctt gggtttgaca tatcccggcg taaattcaga ggtggagccg
     2281 ccacggga
//
LOCUS       DAZGLJ010000382         2271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000382 DAZGLJ010000000
VERSION     DAZGLJ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.390"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            266..457
                     /locus_tag="ACHK49_004964"
     CDS             266..457
                     /locus_tag="ACHK49_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9265421.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(510..743)
                     /locus_tag="ACHK49_004965"
     CDS             complement(510..743)
                     /locus_tag="ACHK49_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265422.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(839..1462)
                     /locus_tag="ACHK49_004966"
     CDS             complement(839..1462)
                     /locus_tag="ACHK49_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO9265423.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(1551..2060)
                     /locus_tag="ACHK49_004967"
     CDS             complement(1551..2060)
                     /locus_tag="ACHK49_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265424.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
BASE COUNT          652 a          583 c          489 g          547 t
ORIGIN      
        1 atgcttttaa tacagttggt tagcttaaaa aatagcaatt tttgatttca gtcattgaaa
       61 gttgcaatgt ttgacgattc aacagcattt aagtgagttt caggatatgg gaaggacatg
      121 aaaggaggag cgcaataaaa tacacaggaa gaaggggaaa atgtttcgta aaaacagaaa
      181 attaacagcg cacatcagga aacgcagtct cctgattcag tgctaccaca tgaaaattta
      241 gttaactgtt accgtactaa aatttatgtc agaagatgtt ccattaccga aagtcaatca
      301 gcgatacaaa gacgatcatg gtgcgcttgt caccctgacc agtgttgagg aaacccgggt
      361 ggtgtttatg caggacggct atccgcatcc ctgcatgcga ccgatgtaca actttctggg
      421 aaaattcaaa cccgaacccc gggaagaaac agaataaaac gaaatccctg ccggcaatca
      481 cactacctga tagcagcata atccacttat taattctcat ccccggagac aagcccagaa
      541 aaccactccg ggtgttcact gtatgccgtg ttcagcgcat ccctggaaac gtttatataa
      601 aagctgtcct gcaatttgcg ggtaacaact tctgccaggc gattccggtt atccaccagt
      661 ccggcataac ctctctctgc cagccactca ttcagttcat gttccagaga cagattgata
      721 atatcataat cggaccttgc cattcccgtt tccttagcac atgttttcag gcacggggat
      781 aaagtataaa gaatgtcata ctgctgctgc cagagcaata ttccccctga tggttgtatc
      841 agagatggta tgccggtact ttgcgggttg ttcccgtcag gttatcgtgc acctccacgg
      901 tcgtggtcac caccggcatt ccggcctttc tcagcctcag aacatcagcc gcaatacgct
      961 gactgccaaa ccagaacagg ccgtccagtg cagtcaccag taaacccgcc tccagtgcat
     1021 acttcagccg ttcacggggc gctttcactt cccgggcaac ctgctgatat ggcaatgatg
     1081 tgttttcatt cccaaccacg cgacgaatac ggtgagacag atgataccgg tatgtatccg
     1141 gcacaccaga aaggctggcc ttcaggctgt acacgcagcc aaaccgttta tcgttaaaca
     1201 ccacattttt ctggctgatg ccccattctt cacgcagcgc ggcaatcaat tgcggtgtac
     1261 gggtaagcag caagcgaaaa ggcagttcaa aactggtgac gtaatccaca ttcagcagtg
     1321 caatacgcaa ccgttcttct ggcccggcct ctgtctgccg gcactcctcc agaacatcct
     1381 gccactgccg gcgaagacgg ggggattcgt tccgttctgt aaaacagtat ttatccgcca
     1441 gatagtcaat tcgtgtacgc atactgaaag gaattccgta taaagattca gcagacaaaa
     1501 ctttatcagc cagtaaaaat taacggaagc attgatattc ctcccgacaa tcaccggatg
     1561 attgttgcaa taacctgcga cataaaggac tgaacagccg tttttaacgc cacgtattgc
     1621 cctgatgaac caggccgggc cacccgaaaa catctttcca cccggcattg tccgccagca
     1681 cttatcaccg accaggtctg ttgcagtaaa tccaccagtt gcgcacattc ttcatcagcc
     1741 atgacgccga ctcgcggatg gcaaatactc gtctggctga gcagcaacag acatcgtatc
     1801 tgtgctcctt catgttgttc ccgcaaccag cgtaatacca ccggcgctga tgggctgacc
     1861 gtgtgattat gctccgtaat acgttcgtgt acccgtcggt gaaaggaact acaaaatgtc
     1921 tgaatctgtt gaccatagat tccttctgcc gaatagagcg tgaagtgaaa tgccaacaga
     1981 tttggccagt ggtccaccgc ctgctgaaca aaacgccgga tttcccccgg ctctgaaatt
     2041 aaggcttcgg ttatttgcac gaaatgttcc ttttcaaccc ctgttgaatt ttgtcaagcc
     2101 ggtgcagaca cagtaaagcg cgcatcaaca cctgaaaggt acgatacaaa cacaagttac
     2161 aaacaataag aacggaagga caattaatca gtgaggaata ataactaaat taaccaaaag
     2221 aatgttactg acaatactac caatacttaa atagcaatat attaattaat g
//
LOCUS       DAZGLJ010000383         2271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000383 DAZGLJ010000000
VERSION     DAZGLJ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.391"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1304
                     /gene="tssI"
                     /locus_tag="ACHK49_004968"
     CDS             <1..1304
                     /gene="tssI"
                     /locus_tag="ACHK49_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO9265425.1"
                     /translation="NTRTEVSTLCISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFEQE
                     GQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQMDGWRNNAETARGMSRSPEIWPGRRI
                     VLTGHPQANLNREWQVVASELHGEQPQAVPGRQGAGTALENHFAVIPADRTWRPQPLL
                     KPLVDGPQSAVVTGPAGEEIFCDEHGRVRVRFNWDRYNPADQDSSCWIRVAQAWAGTG
                     FGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYMG
                     SGFNELKFDDATGREQVYIHAQKNMDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNR
                     LLTVEKGHKITGVLKGSLSEDVFQDRGTIAGSVHVDAVNNGGEGDGIQAYTAIKEILL
                     AVEESKIALTPDGIQLQVGESTVIRLSKDGITIVGGSVFIN"
     gene            1332..1793
                     /locus_tag="ACHK49_004969"
     CDS             1332..1793
                     /locus_tag="ACHK49_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO9265426.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            1813..>2271
                     /locus_tag="ACHK49_004970"
     CDS             1813..>2271
                     /locus_tag="ACHK49_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO9265427.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNINGKPAARA"
BASE COUNT          581 a          602 c          688 g          400 t
ORIGIN      
        1 cgaacacccg taccgaggtg agcaccctct gcatcagcca gttccgctac agcgcacaaa
       61 tccgcccttc ttccgtggtg accaaagact acacctttaa acgccccggc tgggccggac
      121 gttttgaaca ggaaggccag caccaggact accagcgcac gcagtatgaa gtgtatgact
      181 accccggacg tttcaagagc gcccacgggc agaactttgc ccgctggcag atggacggct
      241 ggcgaaacaa cgcagaaacc gcgcggggaa tgagccgctc gccggagata tggccgggac
      301 ggcgaattgt gctgacgggg catccgcagg cgaacctaaa ccgggaatgg caggtggtgg
      361 caagtgaact gcacggcgaa cagccacagg cggtgccagg acggcaggga gcggggacgg
      421 cgctggagaa ccattttgcg gtgatcccgg cagacagaac atggcgacca cagccgttgc
      481 tgaaaccgct ggtcgacggc ccgcagagcg ctgtcgtgac aggaccggca ggcgaggaaa
      541 tcttctgcga cgaacatggt cgcgtgcggg tgaggttcaa ctgggaccgt tataacccgg
      601 cagaccaaga cagttcgtgc tggatccgtg tggcacaggc gtgggcaggc accggttttg
      661 gccacctggc gataccgcgt gtgggtcagg aggtgattgt agacttcctc aacggcgatc
      721 cggaccagcc gatcattatg gggcgcacct accaccagga aaaccgcacc cccggcagcc
      781 tgccgggaac aaagacgcag atgaccatcc gctccaaaac gtacatgggc agcggattta
      841 atgagctgaa gtttgatgat gcgacaggga gagaacaggt ctacatccac gcgcagaaga
      901 acatggatac cgaagtgctc aacgaccgta ccaccaccgt aaaacacgat caccgcgaaa
      961 ccgtaaaaaa tgaccagacg gtcacgatcc aggaaggtaa ccgccttctt acggtggaaa
     1021 aaggccacaa gatcaccgga gtactgaaag ggtctttatc tgaggatgtc tttcaggaca
     1081 gaggcacgat tgccggttcg gtgcatgttg acgctgtaaa caatggtggc gaaggcgacg
     1141 gtatacaggc ttatacggcg attaaggaaa ttttgctggc tgtggaggaa agcaaaattg
     1201 cgctgacgcc ggatggcatt cagctacagg tcggggaatc gacggtaatc aggctgtcga
     1261 aggatggcat caccatcgtg ggcggttctg ttttcatcaa ctgattcact cagattatca
     1321 gggagtattt tatgcagttc acttttaatg aaggccacat tcaactgccg tctcaatggc
     1381 aggatcagtc aatgcaggtc ctggtgtcca cagataacag tggcatcaac ctggtcatta
     1441 cccgcgaagc cgttccacag ggaaccctga caccagagct gtaccaggaa acgctggcgc
     1501 tgtaccaggg caaacttgat gggtacaccg aacatgcctg ccgggaaatc actctggcag
     1561 aggccccggc ctggctgctg gattattcct ggaacggtcc ggaagatgag gggaatcagg
     1621 ggcggattag ccagatagcc gtattccagc ggcgtggaga tacgctgctg accttcactt
     1681 tcagcacctc cttatccctg aagaacagtc aaaagacgat gctgctggag gtgataaaga
     1741 gcttcacccc gttgccaccg gagaatgaca tccaaaagga ccagccgcgc tgacataaac
     1801 gagggataac acatgagtga aggaccaggc gggccacagg gagcgaccgc aggcggtacg
     1861 ctggcaatgc gaatgctgtc acagcaggcg atggtcgcca gccagatgaa acgggcagcc
     1921 aacgacaaag ccattgcaca gatgctggca gcaaagaagt ccggcccacc tgccgccagg
     1981 ctgggcgatg aaattcagca taaaagtttt ctgggggcac tggcaggggc cgtgctgggg
     2041 gcgatagtga ccatcgcaga aggttgcctg attatggccg cctgcgccac cggcccttat
     2101 gcgctggttc tggtgcctgc gctgatgtat gccagctata aggcgagtga ttatgtggag
     2161 gagaaacaga accagcttga atcatggata aacagctttt gtgacacgga cggcgccatc
     2221 aataccggtt ctgaaaatgt aaacattaac ggaaagcccg ctgcaagggc c
//
LOCUS       DAZGLJ010000384         2241 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000384 DAZGLJ010000000
VERSION     DAZGLJ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.392"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>2241
                     /gene="agn43"
                     /locus_tag="ACHK49_004971"
                     /gene_synonym="flu"
     CDS             <1..>2241
                     /gene="agn43"
                     /locus_tag="ACHK49_004971"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO9265428.1"
                     /translation="TGAFVVASELARARGKRGGVAVALSLAAVTSLPVLAADIVVHPG
                     ETVNGGTLANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQWVQDGGTANKTTVTSG
                     GLQRVNPGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHEGAIATGTVINDKGWQV
                     VKPGTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINKNGRQIVRTEGTANTTV
                     VYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQIVKNGGVAGNTTVNQK
                     GRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVVEGKADNVVLENGGRLD
                     VLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSGAAVSGTRSDGKAFSIG
                     GGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAGTTTLNNGAILTLSGKT
                     VNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLTQKAVNLNEGTLTLNDS
                     TVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIPDNATVQSVVDDLSHAG
                     QIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNADRLVIDGGRATGKTIL
                     NLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRLQAGAFNYSLNRDSDESW
                     YLRSENAYRAEVPLYASMLTQAMDYDRIVAGSRSHQTGVNGENNSVRLSIQGGHLGHD
                     NNGGIAR"
BASE COUNT          547 a          606 c          694 g          394 t
ORIGIN      
        1 acgggcgctt tcgtggttgc ctccgaactg gcccgcgcac ggggtaaacg tggcggtgtg
       61 gcggttgcac tgtctcttgc cgcagtcacg tcactcccgg tgctggctgc tgacatcgtt
      121 gtgcacccgg gagaaaccgt gaacggcgga acactggcaa atcatgacaa ccagattgtc
      181 ttcggtacga ccaacggaat gaccatcagt accgggctgg agtatgggcc ggataacgag
      241 gccaataccg gcgggcaatg ggtacaggat ggcggaacag ccaacaaaac gactgtcacc
      301 agtggtggtc ttcagagagt gaaccccggt ggaagtgtct cagacacggt tatcagtgcc
      361 ggaggcggac agagccttca gggacgggca gtgaacacca cgctgaatgg tggcgaacag
      421 tggatgcatg agggggcgat agccacagga accgtcatta atgataaggg ctggcaggtc
      481 gtcaagcccg gtacagtggc aacggatacc gttgttaata ccggggcgga agggggaccg
      541 gatgcagaaa acggtgatac cgggcagttt gttcgcgggg atgccgtacg cacaaccatc
      601 aataaaaacg gtcgccagat tgtgagaact gaaggaacgg caaataccac tgtggtttat
      661 gccggcggcg accagactgt acatggtcac gcactggata ccacgctgaa tgggggatac
      721 cagtatgtgc acaacggcgg tacagcgtct gacactgttg tgaacagtga cggctggcag
      781 attgtcaaaa acgggggtgt ggccgggaat accaccgtta atcagaaggg cagactgcag
      841 gtggacgccg gtggtacagc cacgaatgtc accctgaagc agggcggcgc actggttacc
      901 agtacggctg caaccgttac cggcataaac cgcctgggag cattctctgt tgtggagggt
      961 aaagctgata atgtcgtact ggaaaatggc ggacgcctgg atgtgctgac cggacacaca
     1021 gccactaata cccgcgtgga tgatggcgga acgctggatg tccgcaacgg tggcaccgcc
     1081 accaccgtat ccatgggaaa tggcggtgta ctgctggccg attccggtgc cgctgtcagt
     1141 ggtacccgga gcgacggaaa ggcattcagt atcggaggcg gtcaggcgga tgccctgatg
     1201 ctggaaaaag gcagttcatt cacgctgaac gccggtgata cggccacgga taccacggta
     1261 aatggcggac tgttcaccgc caggggcggc acactggcgg gcaccaccac gctgaataac
     1321 ggcgccatac ttaccctttc cgggaagacg gtgaacaacg ataccctgac catccgtgaa
     1381 ggcgatgcac tcctgcaggg aggctctctc accggtaacg gcagcgtgga aaaatcagga
     1441 agtggcacac tcactgtcag caacaccaca ctcacccaga aagccgtcaa cctgaatgaa
     1501 ggcacgctga cgctgaacga cagtaccgtc accacggatg tcattgctca gcgcggtaca
     1561 gccctgaagc tgaccggcag cactgtgctg aacggtgcca ttgaccccac gaatgtcact
     1621 ctcgcctccg gtgccacctg gaatatcccc gataacgcca cggtgcagtc ggtggtggat
     1681 gacctcagcc atgccggaca gattcatttc acctccaccc gcacagggaa gttcgtaccg
     1741 gcaaccctga aagtgaaaaa cctgaacgga cagaatggca ccatcagcct gcgtgtacgc
     1801 ccggatatgg cacagaacaa tgctgacaga ctggtcattg acggcggcag ggcaaccgga
     1861 aaaaccatcc tgaacctggt gaacgccggc aacagtgcgt cggggctggc gaccagcggt
     1921 aagggtattc aggtggttga agccattaac ggtgccacca cggaggaagg ggcctttgtc
     1981 caggggaaca ggctgcaggc cggtgccttt aactactccc tcaaccggga cagtgatgag
     2041 agctggtatc tgcgcagtga aaatgcttat cgtgcagaag tccccctgta tgcctccatg
     2101 ctgacacagg caatggacta tgaccggatt gtggcaggct cccgcagcca tcagaccggt
     2161 gtaaatggtg aaaacaacag cgtccgtctc agcattcagg gcggtcatct cggtcacgat
     2221 aacaatggcg gtattgcccg t
//
LOCUS       DAZGLJ010000385         2174 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000385 DAZGLJ010000000
VERSION     DAZGLJ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.393"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..188
                     /locus_tag="ACHK49_004972"
     CDS             <1..188
                     /locus_tag="ACHK49_004972"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265429.1"
                     /translation="GFLHPQNIRRPPERAVRNETLFRKKLPTKEQNNRVSVFHSVYSV
                     DDTDIPAITGLDNWKDI"
     gene            109..273
                     /locus_tag="ACHK49_004973"
     CDS             109..273
                     /locus_tag="ACHK49_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO9265430.1"
                     /translation="MFFILFIVLMIPISPLLLVWIIGRTFEPVIELYTDVTWESFSAL
                     HNKINPYKEN"
     gene            275..832
                     /locus_tag="ACHK49_004974"
     CDS             275..832
                     /locus_tag="ACHK49_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO9265431.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            1123..1278
                     /locus_tag="ACHK49_004975"
     CDS             1123..1278
                     /locus_tag="ACHK49_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9265432.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            1398..1742
                     /locus_tag="ACHK49_004976"
     CDS             1398..1742
                     /locus_tag="ACHK49_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFO9265433.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            1864..2136
                     /locus_tag="ACHK49_004977"
     CDS             1864..2136
                     /locus_tag="ACHK49_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265434.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
BASE COUNT          593 a          481 c          577 g          523 t
ORIGIN      
        1 agggattcct gcaccctcag aacatcagga ggccgcccga aagggcggta agaaatgaaa
       61 cattatttag aaaaaaatta cccacgaaag agcagaacaa cagagtttct gtttttcatt
      121 ctgtttatag tgttgatgat accgatatcc ccgctattac tggtctggat aattggaagg
      181 acatttgaac cagttattga gctatatacc gatgtgacat gggaatcatt cagcgcactg
      241 cacaataaaa ttaatccgta taaggaaaac tgatatgagc actattacca gagaacgcgc
      301 ggagattaaa tcatacatca caggcttcct gagcgactcg gcgcacgata acaagtcttc
      361 agacagcctg ctggctaatg tgtttcgtat cgcgctggca tcactggaag cagagccgat
      421 agcaatggta gtgcctgatg aaatggattt gcttacctgc catctcgacg gtgtaactaa
      481 aacatatgct gatggctgga acgcctgccg cgtcgccatg cttcaggccg gaaactttcg
      541 ggaaaataag aattcgtcaa ccaacaattt tcgggaaatc tcggaaacgt caaccagatc
      601 tccgataact ctggatggct ggataagctg tactgagcga atgcctgaaa agagccagaa
      661 cgtgcttatt tcgatgaata tcgatagcga ggctgggcca ttaatatatt ccgcacgcta
      721 tctcggaggc acgttccggc gcggaggtat agcagttagt ccgggtaatg atcttaggca
      781 agcaacccac tggatgtcgc taccagaacc gccgcaggag gtgaatcaat gacctggcct
      841 gaagcattca caacggtagg aattgcgatg gcggtggcgc tggtggtgta ttcgatttgc
      901 cgctggggat aaaaacggtt tgcgggaaaa ggatagttaa gtagaattgc agcgggtgct
      961 tgaggctatc tgcctcgggc atgaacacca acggcagata gagaaaagcc ccagttaaca
     1021 ttacgcgtcc tgcaagacgc ttaacattaa tctgaggctc aatccatgct gaacacatgt
     1081 aggttagcct cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg
     1141 atgttaatcg ccctgatcgt catctgttta accgtcatag tgacggcact ggtaacgagg
     1201 aaagacctct gcgaggtgcg aatccgaacc ggtcagacgg aggtcgctgt cttcgtagac
     1261 tacgaatctg agaagtaaga gaccaggcgg gggagtaatc tcccgccacc tctgatgtgt
     1321 caggcatcct caacgcaccc gcgctttacc atactgaaaa tgctgtttga atgttcatct
     1381 ctgaaagagg actatgaatg aaaaaggtat tgattgcagc acttatttcc ggtgtgtctt
     1441 ttggcgcttt tgcacagcag ggtggtttcc aggggccaga agcagagcgt tcaacagtag
     1501 cgcaggcaaa agaactgaag gatgatgcat gggttatcct tgaagggagc atcgttaaaa
     1561 aagtgggtga tgaacgttat gagtttcgtg acaatagcgg gacaattgtc acggatattg
     1621 atgacagcgt atgggccggg cagaatgttt ctccgaaaga caaagtaaga attgagggtg
     1681 aaattgataa agacctgagc agtgttgaag ttgatgtaaa ggcactgaaa ttattaaagt
     1741 aaccgcccct gcttgttaag cccgtcttac tgacgggttt tctgtttgta cattccggcg
     1801 tattgcctta caattcgcgc agtcagcctg aacaactgac acctgctgtc accggagaat
     1861 ccgatgacac aacacataaa atcccacaat tctgaagccg acccggaaat taagcagggg
     1921 aggcgttttc gtgcgcctca gtatggctgg tttcactatc tgttctgtac gatcgatgag
     1981 gcagatatgc ttcaagaggc gtatctgcgt cgcggtgtcc gtgtggagcg gagtctgaac
     2041 gctgatcgtc tgacctggac cgtttctgta tatcttcctg ttcgtgcaca tctgccacgg
     2101 acacatgcct gctaccgtca gcgcgtctgg aggtaatgtg cgggtattac ttcgacctgt
     2161 tctggttccg gaac
//
LOCUS       DAZGLJ010000386         2170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000386 DAZGLJ010000000
VERSION     DAZGLJ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.394"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..1708)
                     /locus_tag="ACHK49_004978"
                     /pseudo
     CDS             complement(1..1708)
                     /locus_tag="ACHK49_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1654..1875)
                     /locus_tag="ACHK49_004979"
     CDS             complement(1654..1875)
                     /locus_tag="ACHK49_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265435.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQDVT"
     gene            complement(1920..>2170)
                     /locus_tag="ACHK49_004980"
     CDS             complement(1920..>2170)
                     /locus_tag="ACHK49_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265436.1"
                     /translation="NAPDIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSM
                     FQTGSVAIRAERWINWRRRRTAAVAVITGVNYGSASGG"
BASE COUNT          438 a          707 c          531 g          494 t
ORIGIN      
        1 agcatcggac gcacatcatc gggggtgata cttttgccgt ccgccggtac cggaatattc
       61 gcaaccgcat cattcaccgc ctgctgcagt acatccggat cataatcacg accatcacgc
      121 ggtaccggaa tggtccccac agcgtcatcc accatcgcct gcagaaccgg atgtacctca
      181 tccaccgtca catgcttctg taataccgcc gacagggaag ccagtttctc ttcaaacgct
      241 tgtgcctgcg cggccatctt cccctcaaat gtgcgctgta aatccgccag caccgtggag
      301 aattcttcgc ccagtgcacg aataatggac agttcccgtt ccgtcatttt ctcagtatcc
      361 ccctgaacat cgctttcact gcatcatgct ctgtttcact gattgcctta ttaccgtcag
      421 atgcgccgtc aggcagttgt gctgaaactg ttttcccggc cgacgcgaac gggtcctcac
      481 gggcatcacg acgggacagc gcctccagac tgtagttctg ctgctgaaga tacagtgcat
      541 caccaccggc aaggggcggc aggttctcac gtttacgggc ctcattgggc gtgagaagcg
      601 tatttttcac tgattcaccc agtgttttca tgcgccgttc gctgtccatt ctcagcagcg
      661 tggtgacgtc aaactccgtg ctctcgtttt cccccgtttc cagcgcctca tccagtaaca
      721 gctcaatgga ctcaatcagc gtctgcagac actgggaata atactgctgc tccagcgcct
      781 ccacgttgtc actggaaggc gggtggccaa cgccaatctt gtaggccggg acacggaaca
      841 ccgaacagac aatttcagcc gtcattttca gttgttccac cgtctgcgca tccaccggtg
      901 aaaacgtcgt ggggctgtat tttgccccgt tgctcagtat ggccgtcttc ccggcatttt
      961 cgcccgtata tccgctgtcc cagttcccct tcagtttttt cgcgttttct tccgtaatac
     1021 tgccggggac ctcaatcacg cctgatggtc gcccgccatt tctgaaaaaa tacgtcgaat
     1081 ttgcctgaat atgatgcccc tgcatggcgg ccagtcctgc ggcatacacc ggcggcagcc
     1141 ccacaagcgg atgaaaaaaa cagttaaacc ggtcgtggat cacctcccgg gcaggcaccg
     1201 tcaccgactc tgtgatccca cagttccggt ccggcgtgat gcggtagaac acgtcgccgt
     1261 catccgccac cagaggttca acccggttcc agtccagaat acgcagttct ttgatctgcc
     1321 cccgggggtt gcggattttc agcaccaccg tattgccgtg acgcaatttg gaattcagcc
     1381 acagttcaaa aaactggatg cgattctgct gcgcattagg acgacgacag agacgggcag
     1441 tatctccctg ccgcttttcc cggcgtattc cctgtacatc agtctgcatc aggcgaagcc
     1501 gcattttggc aatatcctgg gatatcagcg aaatgcatga aaacaccgca tgaaaggaga
     1561 ggacactttc cggatcggct ttcacaccct gctgccaggc accggcaaaa ggctcagcca
     1621 ccgcctgaaa cagggaccgc cagcccactt ctcttacgtc acgtcctgat ttctggtttt
     1681 ttcgggttcg ccgcaaaagg ttccacattc gccatgctcc gcatcacgtt tctttttctg
     1741 acctgccgga cgtcgcactg tgatgtactc cgccttcccc aggcgaacca gcacctccgc
     1801 acacggctgt gccacatcac ggatatcccc ggcccgggca tcatgcgtgc cctgcagata
     1861 tcggatcttt gccataacct gttacgggag gcgcacgcct cccgtcctcc tcatcagact
     1921 cagccgccgg acgcactgcc gtagttcact ccggtgatca ccgccaccgc cgcggtacgg
     1981 cgacgacgcc agttgatcca gcgctccgca cggatggcca cgctgcctgt ctggaacatg
     2041 gaaaccagct ccaccgggga cggcgtggta ctgtcgccgg tcggctcaga ctgcatttcc
     2101 agtgatgcct cgcgggacat atccactgcc acgccgccgt catccgccag ataaatatcc
     2161 ggggcattca
//
LOCUS       DAZGLJ010000387         2146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000387 DAZGLJ010000000
VERSION     DAZGLJ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.395"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            231..1277
                     /locus_tag="ACHK49_004981"
     CDS             231..1277
                     /locus_tag="ACHK49_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO9265437.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
     gene            1518..>2146
                     /locus_tag="ACHK49_004982"
     CDS             1518..>2146
                     /locus_tag="ACHK49_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO9265438.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLLAVERAFSSQVSVIEGPPGTGKTQTIL"
BASE COUNT          555 a          455 c          569 g          567 t
ORIGIN      
        1 agctggtttg ttactcagca ttctggtacc ggttgatgcg ggtaaatacc ggaagggcgg
       61 tagcgtgcca tcgcaaaaca tggtgggagg tatcgtcagg aggctggtga ttgttggtat
      121 gacatgaata aaaggttttt cgtttgactg gaaaattatg ttgcaggaac gagaggactg
      181 aggaacaaag cttattccgg cgctgtccgt ccttgctgtc actgacactg atgtctgctt
      241 tattatcgcc tttatctctt caggcagcgg atgtccggcg tagcggagat gaagcattta
      301 tcattcagca gcagcgtcag gaagcccttg agcaacaact gacgccttca gcccctgatg
      361 ttcgcctttc tgcacctggc tcttttgccc ataagattaa ttttcctgtt gaaacgccct
      421 gttttcagat taaacagacg gaactgaagg gggctgatgc gttaccacac tggctgcctt
      481 tacaaaaaat cgccaacggg gcggtcgggc attgcctggg ggcgaaagga attaatctgc
      541 tgatgagtac attgcagaac cgtctggtcg atcatggtta tgtcaccacc cgtgttctgg
      601 caccttcgca ggatttaaaa agcggtatcc tccggctggt tattattccc ggtgttgtgc
      661 gacatgtgcg tctgacaccg gacagtgatg actatattca gttgtattcc tcattcccgg
      721 cacacgaagg ttctctgctg gatttacggg acattgagca ggggctggat ttaggtaaca
      781 gccggataca gggacaacat actgagctga atgcaaccag tggaaatctg tctacacaga
      841 atgcgcaact gagtgccgat acgctttccg cccggactgc cgggcagttc agcagtaatg
      901 gcggtacgat aaatgccgac acactgcaga tatcggcaca aagcctgtca aatcgtaaag
      961 gcagtctgat tcagacggga acaggggatt tttcgctgag tctgccggga agcgtggata
     1021 accgggaagg gctgcttgcg gcaaatggcg cggtgcgtct ggatgcactg agccttgata
     1081 atcgcaaggg gaaagtgcag gcggagcagt caccctccct tcagaaatcc ccgcccacgt
     1141 ttctgaaacc gtttgtggct ggtgtctgtg cggcattgct ggcggtcagc gtggctattc
     1201 cgggatggca gtttctgaca cagccatcac cggaggagca gcattttacc tgggggaatg
     1261 gttgtaaaaa gcagtgaaaa cagtaaatac agagtatcgt gatgaattag tgctcagggg
     1321 ctaccagtat tcaataacca gcgtatcggc atcgcattcc tgtaattctg gtattgactg
     1381 ttatgactaa attcaatgta tatatggcag gcagaaatat gttaacagtc ccggagttgt
     1441 ggaaaatcct ccggagctaa tatcgggagt gccttaacaa ttttccgtaa gttaaatgat
     1501 aatttctgag taagaatatg gtcacgattt gtgttgacgg tgaaaataag actcataaaa
     1561 ttaccgactg gacgctgtgg gctggaaatg acgacagaga ggttatgctg acctgtcatt
     1621 ttcgcagcgg gagaaaatat actcgtccgc tttctgtatg tcagataacg ccaactgtaa
     1681 atttgcgaaa tgttttcctc gaaaggaaag ggaacgctgt aacatcccgg gctgaactgg
     1741 ttattatata tggtgataaa tatgctgctg tttattatcg cgagggtgaa cgaccctaca
     1801 tcatgaaaac gaccggtctt gactttcaac agtgttcagc atttacagaa catgccgtgt
     1861 ttaattactt gtgtagggtg gctaatgaac gtatttttta tgcccgcggt aataacagaa
     1921 atatcgatga gaatatcctt aggcagataa agaaaatagt tcctcatccg gataccgcct
     1981 tgcatgcata ctgttcagga cagagtaaaa aacgtgattc gccatggggt ttgatttttc
     2041 cgtttggtct taatgaaagt cagttgctgg ctgtcgaacg ggcattttcc tcacaggttt
     2101 cagttattga aggaccacct ggaacaggta aaacacagac tatact
//
LOCUS       DAZGLJ010000388         2122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000388 DAZGLJ010000000
VERSION     DAZGLJ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.396"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..109
                     /locus_tag="ACHK49_004983"
     CDS             <1..109
                     /locus_tag="ACHK49_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HFO9265439.1"
                     /translation="VKQLPVMGGRPLNRAQALAKIAELKAKFGLKGASV"
     gene            106..396
                     /locus_tag="ACHK49_004984"
     CDS             106..396
                     /locus_tag="ACHK49_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO9265440.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATKEEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            469..675
                     /locus_tag="ACHK49_004985"
     CDS             469..675
                     /locus_tag="ACHK49_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265441.1"
                     /translation="MVELIFSALRILGAMWMVATFIVVASSFVRLVGEGKDLVGVLFG
                     SIFLWVIIGVAPVAVAKMAWRFVS"
     gene            683..1129
                     /locus_tag="ACHK49_004986"
     CDS             683..1129
                     /locus_tag="ACHK49_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO9265442.1"
                     /translation="MKQIYMLRNEAIRNNAIDAILSLPIDDKSPHEVHIKEPKRSNPQ
                     NRLMWALLQDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSVPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGRAA"
     gene            1126..1653
                     /locus_tag="ACHK49_004987"
     CDS             1126..1653
                     /locus_tag="ACHK49_004987"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO9265443.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            1650..1832
                     /locus_tag="ACHK49_004988"
     CDS             1650..1832
                     /locus_tag="ACHK49_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO9265444.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRYRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
BASE COUNT          577 a          442 c          594 g          509 t
ORIGIN      
        1 agtaaaacaa cttcctgtca tgggcggtag acctctaaat cgtgcacagg ctctggcgaa
       61 gatcgcagaa ctcaaagcta agttcggact gaaaggagca agtgtatgac gggcaaagag
      121 gcaattattc attacctggg gacgcataat agcttctgtg cgccggacgt tgccgcgcta
      181 acaggcgcaa cagtaaccag cataaatcag gccgcggcta aaatggcacg ggcaggtctt
      241 ctggttatcg aaggtaaggt ctggcgaacg gtgtattacc ggtttgctac caaggaagaa
      301 cgggaaggaa agatgagcac gaacctaatt tttaaggagt gtcgccagag tgccgcgatg
      361 aaacgggtat tggcggtata tggagttaaa agatgaccat ctacatcact gagctaataa
      421 caggcctgct ggtaatcgca ggccttttta tttgggggag agtaaatcgt ggttgagttg
      481 attttttctg cattgaggat tctcggtgct atgtggatgg tggcgacgtt cattgtggtt
      541 gccagcagtt ttgtccggct ggtaggcgaa ggtaaagacc tggttggtgt gcttttcggt
      601 agcattttcc tgtgggtgat tatcggtgtt gcgcctgtcg ctgtagcaaa aatggcgtgg
      661 cgttttgtga gttgaggtga caatgaagca aatatacatg cttcgcaacg aagcaatcag
      721 aaacaacgcc atagacgcaa tactctcact tccgatcgac gacaagtcac ctcacgaagt
      781 ccacattaaa gaaccaaagc ggagcaatcc tcaaaaccgc cttatgtggg cgttattgca
      841 ggacgtatca cgtcaggtgc tttggcatgg acagagactt gcgccggagg actggaaaga
      901 cctgttcact gccctgtggc ttaagaccaa aaaactggag caacgaagtg tgcctggtat
      961 cgatggtggc gttgtcatgc ttggcgtgcg taccagcaaa atgcgaaagg ccagcatgac
     1021 tgagcttatc gaaatcatgt tttggttcgg ctcagagcgc aacgtgcggt ggagtgatga
     1081 ctcccggcga gagtatgaat ggtcacaacg aaaaggtagg gctgcatgac tatcaaatca
     1141 aatacaccag cacacgacaa ggactgctgg caaacgccgc tttggctttt tgatgcgctg
     1201 gatattgagt ttggattctg gctggattca gctgcgagcg acaaaaatgc tctgtgcgct
     1261 cactggctaa ctgaggccga cgacgcactc aattctgagt ggataagcca cggtgcaatc
     1321 tggaataacc caccgtacag caatatcagg ccgtgggtgg aaaaagccgc tgagcagtgc
     1381 atacaacagc gacagacggt agtgatgctt gtgccagagg atatgtctgt cggatggttc
     1441 agcaaggctc tggagagtgt tgacgaagtt cgcattatca ctgatggacg gattaatttt
     1501 atcgaaccat cgacagggct ggagaagaag ggaaacagta aaggctccat gctgctgatt
     1561 tggcgaccgt tcatcagtcc tcgacggatg tttactaccg tatccaaagc ggcattgatg
     1621 gcgatcgggc agggcgtcag gagggcggca tgaggcgaca gcgacgaagt ttcaccgaca
     1681 tcatctgcga aaactgcaaa taccttccaa cgaaacgcta cagaaataaa cgcaagccaa
     1741 tcccaaaaga atctgacgta aaaaccttca attacacggc tcacctgtgg gatatccggt
     1801 ggctaagaca tcgtgcgagg aaaacaaggt aattgactaa aatcgaagtt acgaacaaga
     1861 aagcgtcgag cgggcttcag tgtacactga gtggattcta tctaggctta gtgcatacag
     1921 aagattgctg gtaaaggaca tgccaggcaa aacgatgagg actgatattt atgaaaacat
     1981 ctgatttttt actgttcttg catgcggtac aggaggggct ttgaccgggc attttatcgt
     2041 gaatattttc acttggtatt tctttggttt tagagattac ttcactcgat gggttttaaa
     2101 tagttttcgt cggtttatcg gg
//
LOCUS       DAZGLJ010000389         2108 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000389 DAZGLJ010000000
VERSION     DAZGLJ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.397"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..296)
                     /locus_tag="ACHK49_004989"
     CDS             complement(<1..296)
                     /locus_tag="ACHK49_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO9265445.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEQQATLSAGYLHVSTNVPG
                     SDELNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTHYSDTRWHEDSVRNRWFSV"
     gene            complement(367..>2108)
                     /locus_tag="ACHK49_004990"
     CDS             complement(367..>2108)
                     /locus_tag="ACHK49_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO9265446.1"
                     /translation="TAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTA
                     DSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQLAPGNY
                     RLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEF
                     WFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAV
                     GQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSNEITQTV
                     NKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPDGMQ
                     SQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFSLT
                     PDGRLTAKNADISGSVNANSGALNNVTINQNCTIKGMLEATQVRGDFVKAVSKAFPKK
                     VGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGTRYTGYG
                     FEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTV
                     IVTKKAASGISIR"
BASE COUNT          448 a          610 c          504 g          546 t
ORIGIN      
        1 acgctgaacc agcggttacg cacggaatct tcatgccagc gggtatcgct gtaatgggtc
       61 agctggcgat tcttgtctcc tgcatagctg aacgacgtca ccatccccag tgtgtccgta
      121 aactcataac ggtatttcac gttaatcccg ttcagttcat cgctgccagg aacgttcgtc
      181 gagacatgaa gataccccgc gctcagcgtg gcctgctgct cagacgccca tgcaggcgca
      241 ccggatacgg ccagacagat ggctgcggac aaaatggcgg cataaagttt acgcataatt
      301 acctctcgct tttctgcaat aaaaaaggcg tcattcctga cgccctttat tggggttata
      361 aatatttcaa cgaatactga tgccggaagc agcttttttg gtcacaatca ccgtacagtc
      421 ggtgatattg cctgccccct gattgccttt ctggaaaatc ttaaactcca gagtgacgct
      481 accaccacca ctaggcatat caataactgc actgtaacta ccgggaatgg cccctttagt
      541 ttctctggat gcgattaata cgccgttttt gcgaacttca aaaccatacc ccgtgtatcg
      601 cgtgcctcct gggttatttc cgctccctgg atcgtcatac gctataccgt taaaaataat
      661 gggcggaata ataatctggc ggtcaaagtt atgatcatcg ctgatggtga ctgtaaccgt
      721 accgtttggt gtttccgtgt taccccacgt accgactttt ttcgggaagg cttttgatac
      781 agctttaacg aaatcccctc tgacctgggt cgcctccagc atgcccttaa tcgtacagtt
      841 ctggttaatc gtgacattgt tgagcgctcc tgagttcgca ttcacactgc cactgatatc
      901 cgcatttttc gccgtcagtc gcccgtctgg tgtcagggaa aatgccggag gattaccgcc
      961 gctggtaatg gtgggagccg tcagatattt caggaacact tcattcatga atatctgatc
     1021 gccctgacca acaaacatcg gctttgtgtt gccattcgca ggattaatca tcgcaatcct
     1081 gtctgctgcc agcagcacct gactctgcat gccgtcgggg gtgttctcaa taccggcacc
     1141 gatacccgca atataaaggc gtccgtcctg catctgctgc agcttcactg cccacatgct
     1201 gttcaggtta ttatttgtat caacctgaac cttctgtatc tgctggatcg ctgcactctg
     1261 gtcttccagt ttcttattga cggtctgtgt tatttcattg ctgacatccg ttatggacgt
     1321 cctgatttca gtcaggtcag gcgcaagctg accgttatca atctgcgtcc acagctcctg
     1381 agccagatgg gttttcccta tctcgccttt gaaaaaatcc agatagcctg atgcatcatc
     1441 actcggctga ccgacagcct ctacaaatgc cgatttgcca acggtgttca cactgcggat
     1501 ataaaagtaa taatcatggc ccggtttgat attgatactg gcagctatcc agtacagcgc
     1561 cgtgccaaga tagcgggctg tggtttcaac ctgcctgata tcagcaatcc gcttttccga
     1621 gaaccagaac tcaaactgta ccgtcggatc ataaaccgca agatgcggcg tggcggttat
     1681 ctgaaaatag cccggtgtca gctcaatccg agacggcgct gccggtgcgg caatccggaa
     1741 cgataccgat gccggatcgc cctgctgtcc ccacgcattt accgcccgga ctgtcaacct
     1801 gtagttcccc ggcgccagtt gcgtgaagcg gtatgtggtt tctgtcgtcc tggccgtgct
     1861 gaccagccgc tcactgccgt catccgctgc cacggtcagg cgaagcagga agctcacgcc
     1921 cttcaccacc ttcggcgtgt cccagcgcgc cagcacctga tattccccgc tgtctgcggt
     1981 gacttcggcg gtcaggtgct gcaccgctgg cggcgtgaca ccattcaccg tgccgctctg
     2041 gtcgccgtca aagtgcgccc cgttatccac gatggcttct ttttccggca cgtgctgcac
     2101 cgccgtga
//
LOCUS       DAZGLJ010000390         2104 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000390 DAZGLJ010000000
VERSION     DAZGLJ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.398"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..194)
                     /locus_tag="ACHK49_004991"
     CDS             complement(<1..194)
                     /locus_tag="ACHK49_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFO9265447.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVA"
     gene            complement(200..898)
                     /locus_tag="ACHK49_004992"
     CDS             complement(200..898)
                     /locus_tag="ACHK49_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9265448.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMVEDMQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFHNNV
                     GNFGGFLSINKLSQ"
     gene            complement(898..1227)
                     /locus_tag="ACHK49_004993"
     CDS             complement(898..1227)
                     /locus_tag="ACHK49_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9265449.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(1224..>2104)
                     /locus_tag="ACHK49_004994"
     CDS             complement(1224..>2104)
                     /locus_tag="ACHK49_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9265450.1"
                     /translation="ADKFAQQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNP
                     LALNNVMSEQKKTWAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIA
                     QNMAAMLTGSEQNWRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGG
                     TAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYA
                     TGGYVGTPGSLADSRSQASGTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDE
                     IQTQMRDGGLFSGGGR"
BASE COUNT          417 a          701 c          507 g          479 t
ORIGIN      
        1 gccacaatct ccccctgcat ttctgcctgc agccagtctt ccggcgacat acggaaatac
       61 gcctccggct caccggagat attcacgcag gggaaatatc tttccccctc cggcgtgctt
      121 accacgaagc cgcacgactc cgctggcgca catcgccggg cgtgcgccag aatcgctgat
      181 tctgtctgtg tcatgggatt tactgcgaaa gtttgttaat ggaaaggaag ccgccaaagt
      241 tgccgacgtt attgtggaac ttacaaccgc tcaggcattt gctgcattta tccttcgtga
      301 tatcggacgt tggctggtca tattcatccg cgacagccgg accgctataa ccgcactcgt
      361 caccgcgata ggtccaggtg caggtgttgg ccagcatgat acgtcccgga aaaacagcgc
      421 catccgtttc cgtcggcgtg gacagtacaa aggaggcact caccgcgctc agttcgctgc
      481 actgctcaat gcgccagcgg ctgatcacct cctgctccgg atcggcgtaa ctgtttccgt
      541 tgacgaagtt caccgcatcc agaaaacggg cgtaaacctt acgcctgacc accgttccgc
      601 cgaccagact ctgcatatct tccaccatac cggtgaccat gccgtgcagg ttagaaaccg
      661 tcagtgtcgg acgggcagca ctgcccttgc cgttcagttc aaatcccgtc ccctgaatgg
      721 gatacgcctg atactgtcgc ccctgccagg tgaccggctc acctttttcg ttctgctcat
      781 tacagaaaaa ataacgttct ccaccgacct ctgtcaggtc gatttcccag agcaccacgc
      841 tggccgactg ctccgcacgg gtgcattcat tcagtgtttc ctgccggata tcctgcatca
      901 gttcaccacc tgttcaaact ctgcgctgaa ctcaacacgc agcatactga cccgcgacga
      961 ccattttgcg caggtcacct ttatctgccg ccactcataa ggcggcgtcc acagaaagga
     1021 tttccagccc ccgtgctctt ccagaaacga ctccagtacc gtggcctcct cacgggggac
     1081 agaaagcgtc acgctgtacg ttttcaggtt ggcattcagc ccggcaggcg ctcgctggga
     1141 atagccatca ccaaagcgca cctttcttac agaaggggcc gaagccacat ccataccggg
     1201 tttcactttc cagcggaagg ttttcatcgt ccacctccgg agaacaggcc accatcacgc
     1261 atctgtgtct gaatttcatc acgggcaccc ttgcgggcca tgtcatacac cgccttcaga
     1321 gcagccggac ctatctgccc gttcgtgccg tcgttgttaa tcaccacatg gttattctgc
     1381 tcaaacgtcc cggacgcctg cgaccggctg tcagccagac tgcccggtgt accgacataa
     1441 ccaccggtgg catagccgcg catcagccgg tagagattcc ccacgccaat ccggctggtt
     1501 gcctccttcg tgaagacaaa ttcaccacgg tgaacaatcc ccgctggctc atatttgccg
     1561 ccggttcccg taaatcctcc ggttgcaaaa tggaatttcg ccgcagcggc ctgaatggct
     1621 gtaccgcctg acgcggatgc gccgccacca acagccccgc caatggcgct gccgatactc
     1681 ccgacaatcc ccaccattgc ctgcttaagc agaatttctg tcatcatgga cagcacggaa
     1741 cgggtgaagc tgcgccagtt ctgctcactg ccggtcagca tcgctgccat attctgtgca
     1801 ataccatcaa aggtctgcgt ggctgcactt tttacctgcg acatactgtc cgtggcgctc
     1861 tcttcccact cactccagcc ggacttcagg cctgccatcc agctcccgcg aagctggtct
     1921 tcagccgccc aggtcttttt ctgctctgac atgacgttat tcagcgccag cggattatcg
     1981 ccatactgtt ccttcaggcg ctgttccgtg gcttcccgtt ctgcctgccg gtcagtcagc
     2041 ccccggcttt tcgcatcaat ggcggcccgt tttgcccgtt gctgctgtgc gaatttatcc
     2101 gcct
//
LOCUS       DAZGLJ010000391         2058 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000391 DAZGLJ010000000
VERSION     DAZGLJ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.400"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..184)
                     /locus_tag="ACHK49_004995"
     CDS             complement(<1..184)
                     /locus_tag="ACHK49_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9265451.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDII"
     gene            complement(184..399)
                     /locus_tag="ACHK49_004996"
     CDS             complement(184..399)
                     /locus_tag="ACHK49_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO9265452.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            542..940
                     /locus_tag="ACHK49_004997"
     CDS             542..940
                     /locus_tag="ACHK49_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265453.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNNISDVRMAPPAKFCNDQTELATCP"
     gene            complement(1021..1179)
                     /locus_tag="ACHK49_004998"
     CDS             complement(1021..1179)
                     /locus_tag="ACHK49_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO9265454.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
     gene            complement(1265..2008)
                     /locus_tag="ACHK49_004999"
     CDS             complement(1265..2008)
                     /locus_tag="ACHK49_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265455.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTGYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLST
                     SFNAGSKRFVIYQSAQDKNLQVDIVRTRI"
BASE COUNT          690 a          513 c          342 g          513 t
ORIGIN      
        1 gaatgatgtc ttttccggtg tgtccgtgac atacagtcca tacgccaacg atatcttcgt
       61 atggtatgta gctgacacct tccaggccat cgtcaccact cggaccagtg atgagcacag
      121 acgctatggc aacagcccca ccaccaatag cagcagcaac agccttgcgt aatgatggcg
      181 acattattca cctctcgcag ccttacgctt atcttcttta atcttgaaat aaaggtttgt
      241 cagatacgtc agcaagccaa acaccaggct acccaatacg cctattgcca cccactggga
      301 tggggagact ttgtccagca actgcagtag ccagtatccc gtccccaccg ctgacgtggt
      361 gtatgacaca cctgttgtta ttttttccat ctggtacata ccccgtctcc cgttatccgg
      421 aagctgacaa caataaaaag ccaccagtta attcctgatg gccctgatgc ataaacgtca
      481 taatacctga ctgttatgat tgacaataat gataatgttt atatagaaag gttcccgatg
      541 tgtgttacat atcatttctc cacggggaat atccccacgc cagcgcagac tcttttaccc
      601 gttctcttct gcgctggctc ttttttatta tgctgctgca tttacctctg gcaccatgct
      661 ttctatctca acacaatacg tggtacttct tgtaaccaat atcataacga ttaatcgaca
      721 tagaatttct cccgtgtaca ggaacagagt taaaaagccg gaaccggaat caaatcacag
      781 gatgaccatc tgccagtggc aggtcataaa aaaaaggccg cgccatgcgc agccagaact
      841 cacaaggaaa atgatagaag gaaataacat tagtgatgta cgcatggcgc ctcccgctaa
      901 gttctgcaat gatcaaacag aactcgctac gtgcccttaa aactcgatca tttagcccct
      961 ccaaggagga ttcaccatgc ggttgatttt ttaataaaca gtaaacaaaa aagtcaagaa
     1021 ttattcattc tgttctttca tcatcggcca cagcaatacc acaatgccgc agaccagagc
     1081 gccatcagtc agtaccaaca ttatcctgct ggtgaaatcc atcatcacca tcactaaaag
     1141 caggatcaca acagcaagca gacacagttt ataaaacaat gttcagaaaa cgcattcagc
     1201 atgcctaagg ttctattcct acgaatagcc aacttgcaac ttaaaatatt atttatgcag
     1261 ccaattaaat tctggtcctt acaatatcaa cctgaagatt cttatcttgt gctgattgat
     1321 aaatgacaaa ccttttacta cctgcattga aagaagtaga caaaaccaga caattatcat
     1381 aacgagcaag aacataatac caaccatcat tataattaat catttcatat tctttcttaa
     1441 actgtggttt gtaatatcct gtcagaaatg aaaaaagcca aaaatatgcc acaaaagcaa
     1501 tcatcacaat ctcaaaaaaa tgttttttta taaatggctt atcatagaag catgataccg
     1561 ataaaaatcg cccataagat cttatcgaaa ttgtaaccgc cagcgcaatc gctgctgaca
     1621 gtagcaaaag aggtacctga atcttctgtc tcaatataga aaactcaata attgccggca
     1681 caaacaataa ttccacagca aaataaaggc gaaatacatt tagctcttgc atagaatgtt
     1741 ttcttttcac tgcgaaaaag aatacaacac caatacccca accgataaga aatatagcaa
     1801 tgacgataac tgcaaaaaat aaacttctgg caacatcatc aacacctgca cctacaatcc
     1861 accatgggaa gccgtagtaa aaagaagtac cccatccata gaaataagca ctcccccatc
     1921 caaggcatcc catgtaggca ataaaaagtg aagaactcct gagcagcgca ccatccttca
     1981 taaccacccc aatacaagat gataacattg gcttacaact cataacaaaa gcaattcaat
     2041 gccgtcaaga ggttacag
//
LOCUS       DAZGLJ010000392         2057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000392 DAZGLJ010000000
VERSION     DAZGLJ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.401"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(310..582)
                     /locus_tag="ACHK49_005000"
     CDS             complement(310..582)
                     /locus_tag="ACHK49_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265456.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            742..1275
                     /locus_tag="ACHK49_005001"
     CDS             742..1275
                     /locus_tag="ACHK49_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9265457.1"
                     /translation="MHEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1496..1609
                     /locus_tag="ACHK49_005002"
     CDS             1496..1609
                     /locus_tag="ACHK49_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265458.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            1831..2016
                     /locus_tag="ACHK49_005003"
     CDS             1831..2016
                     /locus_tag="ACHK49_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO9265459.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
BASE COUNT          544 a          422 c          561 g          530 t
ORIGIN      
        1 aacctgtatt tcaaaatcag agaggaccgt cgtaaggtgg cgcggggaga gtagtcgatg
       61 aataaacaat acgaactggt tgtaaaatga atatttctaa ctgaaaaaac gttccatgag
      121 gtaagaaaag gtcacaggca atcaataaca ggacgtgatg aaagaccctt gcatttgtgc
      181 gctttctctt tagatagcag cagatactga aaatctgagt tgtcggggag tcagggatac
      241 agctgtgcaa gagttggtca ttgtgattcc attgaaatcc tgtatgccat gaagggcagg
      301 attttatggc tacctgagct ttggtgatag taagttgaaa attcgcattt tttgctgaca
      361 tgcgtaacga gaatcccata agcagggagg acttaattct tcattaaccc atgcgttgat
      421 attatgtttc agccgttgaa gcatcagcgg tgttaatgtt gtggtaataa tatccagcgt
      481 tttatgtgag atcttaccgt aagggtctgc aagaatgctg cttgttgctt cgttattatc
      541 tgccatcaga agaagtaact ctgatttaac gttttctgtc attagttgta aaaatcttct
      601 gcgcaaactt tctttactgt tcatttatat ggcttcattt gttgtaatct gctgcgtctc
      661 aagggatatg tttatgagag cgaccatgag tgttggatta tatacctaac atatcaaggg
      721 attagaaatc gataaatccc catgcacgaa aaaataaaat acggcctgtc ggctgccgtt
      781 ctggcgctga ttggtgcagg tgcttctgcg cctgaaatcc tcgaccagtt tctggatgaa
      841 aaggaaggta accacaccac ggcataccgt gatggtgcgg ggatctggac catctgccgt
      901 ggtgccattc tggtggatgg taagcctgtt attcctggca tgaagctgtc aaaggaaaaa
      961 tgcgaccggg ttaatgccat cgaacgtgac aaggcgctgg catgggtgga gaaaaacatc
     1021 cgggtgccgc tgaccgaacc ccagaaagcg gggatcgcgt cattctgtcc gtacaacatt
     1081 ggccccggta agtgcttccc gtcgacgttt tataaacgaa ttaatgcagg cgatcgaaaa
     1141 ggtgcctgtg aggcgattcg ctggtggatt aaggacggtg gcagagactg ccgtatccgt
     1201 tcaaataatt gctacggtca ggtctcacgg cgtgaccagg agagcgcgct ggcgtgctgg
     1261 gacatcgaca gatagcagaa tattttcctg aaaaatgacg ttggccaacg cgggtggata
     1321 acacgaaatc ctgaaaactg gtaaaaccta agtgaataaa agtaaaaacc ccgtttgttg
     1381 gcagcaagcg gggttttgtg ttttctgacc ttgagcaagg caagggagaa attatgggta
     1441 gggaggtact ttccctgtga ggaagtataa aagattcttt ctgaggttgt ccattatgaa
     1501 aggcattgaa gtggagacgc cagccagtct ggatttaaca agagcggcag cttttgccat
     1561 tcgtattgtg gccattgctg ttctggtctg ggcaatccgt tggtggtgat atgaaccgtg
     1621 ttctgtgcgt ggttatcatt gtcctgctgg tggcctgtgg tgcgcttagt ctggggctga
     1681 atcattaccg tgatcacgcc atcatctaca aagagcagcg cgataaaaaa gccagtgagc
     1741 tggagctggc gaacgcgaca attactgata tgcagatacg ccagcgtgat gtcgctgcac
     1801 ttgatgccag atactcgagg gaattagccg atgcgagagc tgaaaatgaa actctgcgtg
     1861 ctgatgttgc cgctggtcgt aagcgcctgc ggatcaacgc cacctgctcc ggtaccgtgc
     1921 gtgaagccac cggcacctcc ggcgtggata atgcaaccgg cccccgactg gcagacaccg
     1981 ctgaacggga ttatttcatc ctcagagaac ggctgatggc aatgcagaag caactggaag
     2041 gagcacagga atatatc
//
LOCUS       DAZGLJ010000393         2057 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000393 DAZGLJ010000000
VERSION     DAZGLJ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.402"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..409)
                     /locus_tag="ACHK49_005004"
     CDS             complement(<1..409)
                     /locus_tag="ACHK49_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9265460.1"
                     /translation="MTTVTHNSTTPSVSVTAASGNNPPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRMENYAM"
     gene            complement(501..1319)
                     /locus_tag="ACHK49_005005"
     CDS             complement(501..1319)
                     /locus_tag="ACHK49_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO9265461.1"
                     /translation="MRLASRFGHVNQIRRDRPLTHEELMHYVPSIFGEDRHTSRSERY
                     AYIPTITVLESLQREGFQPFFAYQIRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPSRQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRTIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(1647..>2057)
                     /locus_tag="ACHK49_005006"
     CDS             complement(1647..>2057)
                     /locus_tag="ACHK49_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO9265462.1"
                     /translation="NAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAK
                     HSVRELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
BASE COUNT          463 a          552 c          577 g          465 t
ORIGIN      
        1 tcatggcata attctccata cgacaggcat gatgactgta cgtcatcaga caggcggcaa
       61 taccggcggc ttccgggctc atttcagcgc ggttaccgtt catggcattg aacagtaccc
      121 atgtttcgtc atcatcgtca tccggttcgg gtgccataaa tgccccgccg ttgttcaggg
      181 tgtacagatt ccagatacca ccgcagtagt cttcgcacag acggtccatc cagccgaaga
      241 cacggggctc cagggtcacc cactgtggaa tgaggccaaa atgctgcggc cagaagctga
      301 tgcgctgttc atcagggacg agagtagcaa ccagctgagg tgggttattc cctgatgcag
      361 cggttacgga aacagaaggt gtggtggaat tatgcgtaac ggttgtcatg agattattcc
      421 ttataaaaag taaatgaatg gaagaatgtc cggaggcagg gacggaagtg agtcaggact
      481 gcgctttcag ggaaaaggca tcagcgcatg ctctccagca gtgtttctgc catcacccac
      541 aacgcccggt tgagcttaat gtcggtgtcg atgctgtgaa tggtacgggt atggatacgt
      601 tttcctctgg cactgcgacc ggaaatcccg cctttcagca tattctcctg gatggtctga
      661 taagcactcc acaggtcctt accgtaatcc tcccggcgtc gtggcgtcag aatgtcggcg
      721 gtggtgacgg gctgatgttc gtcaccataa cggtaagtca gtgccgcctg tgccagcgcc
      781 tggcgtgacg gtggcggcag gaccagcgac tgcatggcat cacgcttctc ctctatccgg
      841 tcaaacacgc ccaccacctc gtaagcccct tcgataactt tctccaccac atttccccgg
      901 tgtggaacac gcacttcccc cagagactgg ccacagacgc acccgttctg gcagacgaac
      961 ctgaagtaac ccggcagcat ctggtagctg gaagtaccgt catgggagtt cagcaggatg
     1021 atttcaggga catgttgtcc gtttatctcc ccgtcacggc gcagacgcag catgtgtttg
     1081 gtgtatcccc ggcggcccgg gtcgcgcacg cggatctggt aggcgaagaa gggctgaaag
     1141 ccctcccgct gcaggctttc tagtacggtg atggtgggaa tgtacgcata gcgttcactg
     1201 cgggaggtat gccggtcttc cccaaaaata ctgggcacat agtgcatcag ttcttcatgt
     1261 gtcagcgggc ggtcacggcg tatctggttt acatgaccaa aacgacttgc taatcgcatt
     1321 atttgctcct tatgaatgag ttagcggtat aaatgaaaaa gccgcgtctc ccggagaaga
     1381 cgcggcctga cagatgaaat gaatgacgtt tattgtctga gaagccctcc actggtgagc
     1441 tgcgtatttt catggaagga gatcacccaa tatccccatc gggatcgggt aataactggc
     1501 cagactgtga tcaattcaga tgccatttgg cagtaaaagg gtcttcgggc aataaacgtg
     1561 atgcacaacc cggcatatct gttgatccct gaaagtaaaa ggccccggtc ataatgaccg
     1621 gggcctgaag ggaagtgacc tgattatcag aaagtcacgt tcagtgtggc ctgaccgtta
     1681 taaccttcag cgctgctgcc gctgacactg tgggcataac cggcctgaac gcccagggtg
     1741 atattttccc ggacacgggc ttccagtccg gcctgcaggt ccagtgacgt gccattccgg
     1801 gacggtgaga acgtcatgtt actgccggct gcggctgtac ccatgctcat gtctccccgg
     1861 gagctgaagg tgcggataac agaaggctgt acccaccagt tcaccggcaa ttcacgcaca
     1921 ctgtgtttgg cactgtcgcg cagggtgtca cgggatgagg tgcctttacc aaagttcata
     1981 tcgttgtggc tgcccagacg gaagccggca cgcacatgtt gtgcactgcc atgcccgaac
     2041 ttcacataac cggcgtt
//
LOCUS       DAZGLJ010000394         2054 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000394 DAZGLJ010000000
VERSION     DAZGLJ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.403"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            29..517
                     /locus_tag="ACHK49_005007"
     CDS             29..517
                     /locus_tag="ACHK49_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HFO9265463.1"
                     /translation="MKNKLLIAAVLTGLLSGCVDGNSPSGSQEGVVKADPRYSECNLP
                     DVNERGPIAAGLYISGTLPEGYWLMKPERKMHYKGDGIYQLIYSEKAGAVTMQFATMN
                     WNPQFTVTGLELPLNQAKTLKKGGMDKNTKVVIPADGKYVWTIQIGPDKKAIAAMVAP
                     CK"
     gene            600..1727
                     /locus_tag="ACHK49_005008"
     CDS             600..1727
                     /locus_tag="ACHK49_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9265464.1"
                     /translation="MAAVILRKVEKIYDNGFKAVHGIDLDINDGEFMVFVGPSGCAKS
                     TTLRMIAGLEDVSGGEIYISDRLVNTLPPKDRSIAMVFQNYALYPHKTVFDNMAFGLK
                     MQKRPKEEIKKIVEEVAEKLEISDLLYRKPKEMSGGQRQRVAVGRAIVRKPDVFLFDE
                     PLSNLDAKLRVSMRVKIAQLHQSLKDEGHPATMIYVTHDQIEALTLGDRICVLNQGRI
                     MQVDTPVNLYNHPANRFVAGFIGSPSMNLIDAVVREEDGKLYIDIAKNIRLYIPENKQ
                     SVLKAYIDKPVCFGIRPEHISLSLNCKEMNTVTGALSIIENMGSEKYLYFTVNNKEFI
                     AKVNDQSITTADIGKNLYFNLYTSFCHIFDFYTEENLTNYL"
BASE COUNT          645 a          378 c          420 g          611 t
ORIGIN      
        1 agctatacta cttttattga gagtttaaat gaaaaataaa ttgcttattg cggctgtgtt
       61 gactggtctt ctttcaggat gcgttgatgg caattcaccc tctggcagtc aggaaggtgt
      121 cgtgaaagcg gatcctcgct attcagaatg taacctccct gatgtaaatg aaagagggcc
      181 aattgctgcg ggtctttaca tttccggaac cctcccggaa ggctattggt taatgaagcc
      241 agagcgtaaa atgcattata agggagatgg tatttatcag cttatttatt ctgaaaaagc
      301 aggtgcggta acaatgcaat ttgcgactat gaactggaac ccgcagttca ctgtaacagg
      361 tcttgaattg ccattaaatc aggctaaaac actcaagaaa ggaggcatgg ataaaaatac
      421 aaaagtcgtt attcctgccg atggaaagta tgtttggacg attcaaattg gtcctgataa
      481 aaaggcaata gccgccatgg tagcaccatg taaatagtta tttttagcac tccactattc
      541 aacattcgtt gaatagtgga gtatcagtat tccaaaattc ttgttaatga gagcagatta
      601 tggccgcggt aattctacgc aaagttgaaa aaatttatga caatggattc aaagctgttc
      661 acggcattga tctcgatata aacgatggtg aattcatggt ttttgttgga ccatctgggt
      721 gtgccaaatc aactacatta cgcatgattg caggtcttga agacgtatca ggtggtgaaa
      781 tttacattag cgaccgcctt gttaataccc ttccgcctaa agatcgcagc attgcaatgg
      841 tgttccagaa ctatgcgctg tatccccata agactgtatt tgacaatatg gcttttggtt
      901 taaaaatgca aaaaaggcca aaagaagaga ttaagaaaat tgtcgaagaa gttgcagaaa
      961 aacttgaaat tagcgattta ctttaccgaa aaccgaaaga aatgtcgggt ggtcagcgtc
     1021 agcgtgtcgc ggtaggccgt gcaattgtcc gtaagccaga tgttttctta tttgatgagc
     1081 cactatctaa ccttgatgca aaacttcgtg tttcaatgcg agtcaaaatt gctcagttgc
     1141 atcagtctct caaggatgag ggtcatccag caaccatgat ttatgttaca cacgatcaaa
     1201 ttgaagcatt gaccctcggt gatcgtattt gtgtgcttaa tcaggggcga ataatgcagg
     1261 tcgacacacc agttaatctg tataaccacc ctgctaacag attcgttgct ggctttattg
     1321 gttccccgtc aatgaatttg atagatgcgg ttgtacgtga ggaagacggt aaactctata
     1381 ttgacattgc caaaaatatt cggttgtaca tacctgagaa taaacaatca gtactcaaag
     1441 cgtatataga taaacccgtt tgttttggta ttcgtccaga acatatctct ttgagtttga
     1501 attgcaaaga aatgaataca gtcactggtg cattaagtat tattgaaaat atgggcagtg
     1561 aaaaatatct ttactttacc gttaacaaca aagagtttat tgcaaaagtc aacgatcaat
     1621 caattactac agccgatatt ggtaaaaatt tgtatttcaa cctttataca agcttctgtc
     1681 atatatttga tttttacact gaagaaaatc taactaatta tttgtgattg atatgacaat
     1741 aaccccaggt tgaataacgg tattaaacct tacaattcca taaggcttct tactgctcga
     1801 actgtctatt ggtaaatgtc attaactcca cgaactgatt cttatcaagg taattgagtt
     1861 acaaaattcc atccataaaa aagagcatat atgaataaac cgttgataac accgcttact
     1921 cagtgagcct actatttagg tcagagcatg tcttctgacc atagttggag ggcattcaga
     1981 ttcgcagttc acaatgctta cgtactgtag acctgtaacc agcttgagga tgaattacga
     2041 taaatccaca tttt
//
LOCUS       DAZGLJ010000395         2041 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000395 DAZGLJ010000000
VERSION     DAZGLJ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.404"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..157
                     /gene="kilR"
                     /locus_tag="ACHK49_005009"
     CDS             <1..157
                     /gene="kilR"
                     /locus_tag="ACHK49_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HFO9265465.1"
                     /translation="LHEGRTYIASANNIKKRKLYIRNLTTKTCITDRMIKVFLGRDGL
                     PVKAESW"
     gene            157..327
                     /locus_tag="ACHK49_005010"
     CDS             157..327
                     /locus_tag="ACHK49_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFO9265466.1"
                     /translation="MTKKIKCAYHLCKKDVEESKAIERMLHFMHGILSKDEPRKYCSE
                     ACAEKDQMAHEL"
     gene            402..677
                     /locus_tag="ACHK49_005011"
     CDS             402..677
                     /locus_tag="ACHK49_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein RacC"
                     /protein_id="HFO9265467.1"
                     /translation="MITNYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            779..>2041
                     /locus_tag="ACHK49_005012"
     CDS             779..>2041
                     /locus_tag="ACHK49_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFO9265468.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASNDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRELHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDYEEKPQP
                     SGTTAVEQGEAETMEPDATEHHQDTQPLD"
BASE COUNT          641 a          482 c          462 g          456 t
ORIGIN      
        1 tcttcatgaa ggccggacat atattgcctc ggcaaacaat attaaaaagc gaaaactata
       61 tattcgtaac ctgaccacaa aaacatgcat tactgaccgc atgattaaag tcttcctcgg
      121 tcgtgatggt ttacctgtaa aggcggagtc atggtgatga ctaagaaaat aaaatgtgct
      181 taccaccttt gcaaaaaaga cgttgaagaa agcaaagcta ttgaaagaat gcttcacttc
      241 atgcacggga ttttatcaaa agacgaaccg agaaaatatt gcagtgaagc ttgtgccgaa
      301 aaagaccaga tggcacatga actttaatta attgactatt cgaaactgaa tttatgccag
      361 aaatggcagg tattcgctca accttaatta aggagaaaaa catgattacc aattatgaag
      421 ccactgttgt aactaccgat gacattgttc acgaggtgaa tctggaagga aagcgcattg
      481 gctacgtaat taaaacagaa aataaagaaa ccccattcac tgtggttgat atcgatggtc
      541 catcaggcaa cgtaaaaaca cttgatgaag gtgtcaaaaa aatgtgcctg gtgcatatcg
      601 gaaagaatct gcccgcagaa aaaaaagccg aatttctggc aactctaatt gcaatgaaat
      661 taaaaggtga aatctgaaag aaatagcctg cgtatggcgc aggctatgaa cagtgtgtat
      721 ccggcaagat cattcactga acaaaacgaa ttttaatctg agttgaggtt aaaaaacaat
      781 gagcacaaaa ccactcttcc tgttacggaa agcgaaaaaa tcatccggtg aacctgacgt
      841 cgtcctgtgg gcaagcaacg attttgaatc gacctgtgcc actctggact acctgatcgt
      901 taagtcaggt aaaaaactga gcagctattt taaagctgtt gccacgaatt ttcctgtcgt
      961 taatgacctg cccgctgaag gtgagatcga ttttacctgg agtgaacgct atcaactcag
     1021 caaagactcc atgacatggg aactaaaacc gggagcagca ccagacaacg ctcactatca
     1081 aggcaatacc aacgtcaacg gcgaagacat gactgagatt gaggagaata tgctactccc
     1141 aatttctggc caggaactgc ccattcgttg gcttgctcaa cacggcagcg aaaaaccggt
     1201 aacgcacgtt tcacgcgacg gactccaggc attacacatt gctcgggctg aagaactacc
     1261 ggctgttact gccctggctg tttcccacaa aaccagcctg ctcgacccgc tggaaattcg
     1321 cgaactccac aaactggttc gtgacactga caaagttttc cctaatcctg gtaattcaaa
     1381 cctgggactg ataactgctt ttttcgaagc atacctgaac gctgactaca ccgatcgagg
     1441 actgctgaca aaagagtgga tgaagggtaa tcgtgtttca cacatcactc gcacggcttc
     1501 cggtgctaat gctggcggcg gaaacctcac cgatcgcggc gaaggtttcg tacacgatct
     1561 gacgtcactg gcgcgggacg tagccactgg cgtactggcc cgttcaatgg atctggacat
     1621 ctataacctt catccggcac acgctaaacg cattgaggaa attatcgctg aaaataaacc
     1681 gcccttttct gttttccgcg acaaattcat caccatgcct ggcgggctgg attattcccg
     1741 cgccatcgtg gttgcgtccg taaaagaagc accaattggg atcgaggtca tccccgcgca
     1801 cgtcactgaa tatctgaaca aagtactgac tgaaaccgat catgccaacc ctgatccgga
     1861 aatcgtggat attgcctgcg gtcgctcctc tgccccgatg ccgcagcgag taacagaaga
     1921 aggaaaacag gattatgaag aaaaaccgca accatctgga acaacggcag ttgaacaggg
     1981 agaggctgaa acaatggaac cggacgcaac tgaacatcat caggacacgc agccgctgga
     2041 t
//
LOCUS       DAZGLJ010000396         2013 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000396 DAZGLJ010000000
VERSION     DAZGLJ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.405"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..504
                     /locus_tag="ACHK49_005013"
     CDS             <1..504
                     /locus_tag="ACHK49_005013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265469.1"
                     /translation="LSFSEFKQALRIRMCSHNDAEHKYEKQNQTAENFVLEPGETLFK
                     IPVTCPICGFTSEELDDSCNNQETTKYVEDDTECARRTIISTSPNSRTNKSHYERVIN
                     PLPQTNKKDAGGQKTQWNNGISVKKRRSIKDKQRSANNCKRFIPVINFHVLKRIYWWL
                     RHCLMNP"
     gene            991..1179
                     /gene="dicB"
                     /locus_tag="ACHK49_005014"
                     /gene_synonym="ftsT"
     CDS             991..1179
                     /gene="dicB"
                     /locus_tag="ACHK49_005014"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO9265470.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            1176..1367
                     /gene="ydfD"
                     /locus_tag="ACHK49_005015"
     CDS             1176..1367
                     /gene="ydfD"
                     /locus_tag="ACHK49_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HFO9265471.1"
                     /translation="MNSAFALVLTVFLVSGVPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            1461..>2013
                     /locus_tag="ACHK49_005016"
     CDS             1461..>2013
                     /locus_tag="ACHK49_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease VIII"
                     /protein_id="HFO9265472.1"
                     /translation="MSKVFICAAIPDELATREEGSVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAK"
BASE COUNT          588 a          439 c          463 g          523 t
ORIGIN      
        1 ctcagcttca gtgagtttaa acaggcactc agaataagaa tgtgctctca caatgacgcg
       61 gagcacaaat atgagaagca aaatcagacc gcagaaaatt ttgttctgga acccggagaa
      121 acgcttttca aaattcccgt tacgtgcccc atttgcggtt ttacatcaga agaacttgac
      181 gactcctgta acaatcagga aacaaccaag tatgtcgaag atgataccga gtgcgcacga
      241 agaacgatta tatccacgag tccaaactcc aggaccaata aatctcacta tgagagggtg
      301 attaatccac tcccccaaac caataaaaaa gatgccggag gacaaaaaac ccaatggaac
      361 aacgggatat ctgtcaaaaa aagacgttcc attaaagaca aacaacgcag cgccaacaac
      421 tgtaagcgct ttataccagt aatcaatttt catgttctta agcggattta ttggtggttg
      481 cgacattgct taatgaatcc ttaaaactgt ggtgatttta aggataccac ctcgcctgac
      541 gtggttaaaa gcaggcacac aacacgaaag cgcacggcga agttcgtctc actgtacggt
      601 gtcgttaaat ttaattcgac cgtgcgcttc cggttgtggc aacccgcaaa atggcgcggc
      661 ggtaagtatg gcggggttat tccttccccg ttgaggacac cgggttgtca ggttgaccat
      721 acgcttaagt gacaaccccg ctgcaacgcc ctctgttatc aattttctgg tgacgtttgg
      781 cggtatcagt tttactccgt gactgctctg ccgccctttt taaagtgaat tttgtgatgc
      841 ggtgaatacg gctaagcgca cgcggaacag ttaaaaccaa aaacagtgtt atgggtggat
      901 tctctgtatc cgacgttaat tgttaactgg ttaacgtcac ctggaggcac caggcactgc
      961 atcacaaaat tcattgttga ggacgcgata atgaaaacgt tattaccaaa cgttaatacg
     1021 tctgaaggtt gttttgaaat tggtgtcact atcagtaacc ctgtatttac tgaagatgcc
     1081 attaacaaga gaaaacaaga acgggagcta ttaaataaaa tatgcattgt ttcaatgctg
     1141 gctcgtttac gtctgatgcc aaaaggatgt gcacaatgaa ttcagcattt gcgcttgttc
     1201 tgacagtttt tcttgtttcc ggagtgccag ttgatattgc agtcagtgtt cacaggacaa
     1261 tgcaggagtg tatgactgca gcaaccgaac agaaaattcc cggtaactgt tacccggtcg
     1321 ataaagttat tcaccaggat aatatcgaaa tcccggcagg tctttaaaac agttccgtaa
     1381 taaatatccg gtttcattct tatatgccag caatggcagg gatttgttca tccttaaatc
     1441 tgtcatgagg ttaaaacaaa atgagtaaag tctttatttg cgccgctatt cctgacgaac
     1501 tggcaacaag ggaagaaggc tctgtggctg tagccacagc cattgaagct ggcgacgaac
     1561 gccgtgctcg agcaaaattt cactggcagt tcctggaaca ttatccggct gctcaggact
     1621 gcgcttataa atttattgtc tgcgaggata aacctggcat accccgccct gccctcgatt
     1681 catgggatgc tgaatatatg caggaaaacc gctgggatga ggagtctgct tcttttgtcc
     1741 cggttgagac tgaatccgat ccgatgaacg tcacttttga caagctggcc cctgaagtac
     1801 agaacgctgt catggttaag ttcgacacat gtgaaaacat caccgttgat atggttatta
     1861 gcgcacagga attgttgcag gaagacatgg caacattcga cggacatatc gttgaagcgt
     1921 tgatgaaaat gccagaagtt aacgccatgt atccggagct taagttgcac gccattgggt
     1981 gggttaagca taaatgtatt cctggtgcta aat
//
LOCUS       DAZGLJ010000397         2000 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000397 DAZGLJ010000000
VERSION     DAZGLJ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.406"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            147..899
                     /locus_tag="ACHK49_005017"
     CDS             147..899
                     /locus_tag="ACHK49_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HFO9265473.1"
                     /translation="MTTVNQIPDANITPSLPPKIREAVDKVKAAKAVWQEERRKQTEA
                     AAMIETIRKRQEDTKTETQALNDEWRNLFRENQGNMTPRMKKLRAEIALGRETLDEFE
                     DLLAAQAAENEFLPWKTADAANHYISEHNRLIETHAVWLWNEFMKEHGQKLIQILGLL
                     KMTLGRSASAVIGVVHTVNDPESVLKQFISEQLTTPALSCNVSSTDDITLPGISIYAD
                     DKAIQDARQSPSPAARSRMLKQRDMVKGGEKK"
     gene            896..1450
                     /locus_tag="ACHK49_005018"
     CDS             896..1450
                     /locus_tag="ACHK49_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFO9265474.1"
                     /translation="MNAGTITQEALNDYRSAIKSWLTLRNTQSTYQLRLAALLDTEEK
                     PAAYASQLEDLRERLAVLEWQINCAARDGLYAHQIVLESCVTSATENFMSEHGDALTD
                     ALAPFLCAPYGLEAAMKILRTAVARQTEMRTPVISAAYKSIIDETGLTVDASMRADAS
                     ASFTPAQHKVFLARLNRLNEKGVC"
     gene            1452..1724
                     /locus_tag="ACHK49_005019"
     CDS             1452..1724
                     /locus_tag="ACHK49_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO9265475.1"
                     /translation="MALKCPECGAVAHARTSAYEAPSVKRSWYQCQNLECSCTFTALE
                     SVDTIIMKPRCNEQESDKAKIPEKQQQTLNRYGSASKLSNRQQIPV"
BASE COUNT          569 a          492 c          499 g          440 t
ORIGIN      
        1 aggaagcgtg tagatcagac atgacacctt tctctggcta gccagaacaa ggtcattgtt
       61 cagtcactga cacaatcctc ctcggtagtg cgcttgtatg gaccccggca caattgacac
      121 aacaacaaaa cactaccgga gcagtcatga ctactgttaa ccagatccct gatgcaaaca
      181 ttacaccctc cctcccaccg aaaattcgcg aagcggtgga taaagttaag gcagcaaaag
      241 ctgtctggca ggaagaacga cgaaaacaaa ctgaagctgc tgcaatgatt gaaactatcc
      301 gtaaacgtca ggaagataca aaaacggaaa cgcaggcgct taatgatgaa tggcgaaacc
      361 tgtttcgtga gaatcaggga aatatgacgc cacgcatgaa aaaactgcgg gcagaaatcg
      421 ccctcggacg cgaaacactg gatgagttcg aagatttgct ggcagctcag gcggcagaaa
      481 atgaattcct gccctggaaa actgcggatg ctgcaaacca ctacatcagc gaacataatc
      541 gcctgattga aactcatgca gtgtggctct ggaatgagtt tatgaaggaa cacggtcaga
      601 aacttattca gatccttgga ctgctgaaaa tgactctggg ccgaagcgct tctgctgtta
      661 tcggtgtagt tcataccgta aacgaccccg aaagtgtgct gaagcaattt atcagcgagc
      721 aactcaccac tccggcactg tcctgtaacg tatcttcaac cgatgatatt accctgccgg
      781 ggatcagcat ttatgcggac gataaagcca tccaggatgc cagacaatcc cccagccctg
      841 cagcgcgttc acggatgctt aaacagcgtg acatggttaa agggggcgag aagaaatgaa
      901 tgccggaacc attactcagg aagcactcaa cgattaccgc tcggcgataa aaagctggct
      961 gacactacgt aatacgcaaa gtacctacca gcttcgtctg gcggctctgc tggatactga
     1021 agaaaaaccg gcagcatatg ccagccagct tgaggatctt cgtgagcgtc ttgcagtcct
     1081 cgaatggcag attaactgtg ctgcacggga tggtctttat gctcatcaga ttgtgctgga
     1141 gagttgtgtt acaagcgcaa cggaaaactt catgagcgag catggtgatg cactcactga
     1201 cgctctggct ccttttcttt gcgctccata cgggcttgag gcggcaatga aaatattacg
     1261 aaccgctgta gcccgacaaa cggaaatgcg tactccggta atttcagcag catataagag
     1321 cattatcgac gaaacaggat taacggtgga tgcatcaatg cgcgctgatg cttcagccag
     1381 tttcaccccg gcacaacata aagttttcct ggcccgcctt aaccgactta atgaaaaagg
     1441 agtgtgctga tatggccctg aagtgtcctg aatgtggcgc ggtcgctcac gccagaacca
     1501 gcgcctatga agctccgtcg gttaaacgct catggtatca gtgccagaat ctggaatgtt
     1561 cctgcacatt taccgcactg gaaagcgtgg atacgataat tatgaagcct cggtgcaatg
     1621 aacaggaatc agacaaagca aaaataccgg aaaaacaaca gcaaactctc aatcgctatg
     1681 gctccgcgtc aaagctgtca aaccgtcagc aaattcctgt ctgattagca aaatacgacc
     1741 acgaagtccc ggtccagtac cgggattttt tacgcctttt tcctggctgg cctgagagcg
     1801 tatgagtgca tatctatggc gcatgaaaac gcatgagtct catgcgccat ttttgacgcg
     1861 aaagcccttg tgtggtggct tctgagacga tttacggggt gcatgaaaac cagtctgtta
     1921 agcgaagcgg gcaggcgggc ggggcattgc gcgcaggaat gtgtataccg ttttagtaat
     1981 gatcgctgac atctcacttt
//
LOCUS       DAZGLJ010000398         1932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000398 DAZGLJ010000000
VERSION     DAZGLJ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.407"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..912
                     /gene="espB"
                     /locus_tag="ACHK49_005020"
     CDS             <1..912
                     /gene="espB"
                     /locus_tag="ACHK49_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFO9265476.1"
                     /translation="TVNSVSENTTGSNAITASAINSSLLTDGKVDVSKLMLEIQKLLG
                     KMVRILQDYQQQQLSQSYQIQLAVFESQNKAIDEKKAAATAALVGGAISSVLGILGSF
                     AAINSATKGASDIAQKTASTSSKAIDAASDTATKTLTKATESVADAVEDASSVMQQAM
                     TTATRAASRTSDVADDIADSAQRASQLAENAADAAQKASRASRFMAAVDKITGSTPFI
                     AVTSLAEGTKTLPTTVSESVKSNHEISEQRYKSVENFQQGNLDLYKQEVRRAQDDIAS
                     RLRDMTTAARDLTDLQNRMGQSVRLAG"
     gene            919..1326
                     /gene="cesD2"
                     /locus_tag="ACHK49_005021"
     CDS             919..1326
                     /gene="cesD2"
                     /locus_tag="ACHK49_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFO9265477.1"
                     /translation="MVDTFNDEVLNHYLEQKGYTIQKEFLCGSAFFIGWRIETSFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTRLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFVMNVNS"
     gene            1363..1584
                     /gene="escF"
                     /locus_tag="ACHK49_005022"
     CDS             1363..1584
                     /gene="escF"
                     /locus_tag="ACHK49_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HFO9265478.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKTIKDLVSTISNRSF"
     gene            1590..1868
                     /locus_tag="ACHK49_005023"
     CDS             1590..1868
                     /locus_tag="ACHK49_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscG/YscG/SsaH family type III secretion system
                     needle protein co-chaperone"
                     /protein_id="HFO9265479.1"
                     /translation="MVNDVSANKILVWAAVAAANHKLPKYAESILNVLPQIIPDKKDI
                     AHLEFIILYGLNRKNDAVKALEDFMDDETSQLLCSLVHENNRSWTLMF"
BASE COUNT          576 a          362 c          443 g          551 t
ORIGIN      
        1 acggttaatt ctgtttcgga gaatactacc ggctctaatg caattaccgc atctgctatt
       61 aattcatctt tgcttaccga tggtaaggtc gatgtttcta aactgatgct ggaaattcaa
      121 aaactcctgg gcaagatggt gcgtatattg caggattacc aacagcaaca gttgtcgcag
      181 agctatcaga tccaactggc cgtttttgag agccagaata aagccattga tgaaaaaaag
      241 gccgctgcaa cagccgctct ggttggtggg gctatttcat cagtattggg gatcttaggc
      301 tcttttgcag caattaacag tgctacgaaa ggcgcgagtg atattgctca aaaaaccgcc
      361 tctacatctt ctaaggctat tgatgcggct tctgatactg cgactaaaac gttgactaag
      421 gcaacggaaa gcgttgctga tgctgttgaa gatgcatcca gcgtgatgca gcaagcgatg
      481 actacagcaa cgagagcggc cagccgtaca tccgacgttg ctgatgacat tgccgattct
      541 gctcagagag cttctcagct ggctgaaaac gctgcagatg ccgctcagaa ggcaagtcgg
      601 gcaagccgct ttatggctgc agtagataag attactggct ctacaccatt tattgccgtt
      661 accagtcttg ccgaaggcac gaagacattg ccaacaacgg tatctgaatc agtcaaatct
      721 aaccatgaga ttagcgaaca gcgttataag tctgtggaga acttccagca gggtaatttg
      781 gatctgtata agcaagaagt tcgcagagcg caggatgata tcgctagccg tctgcgtgat
      841 atgacaacag ccgctcgcga tctcactgat cttcagaatc gtatgggtca atcggttcgc
      901 ttagctgggt aattgatcat ggtcgatacg tttaatgatg aagtgcttaa tcactatctt
      961 gaacaaaaag ggtacacaat acagaaggag tttctttgtg gcagtgcctt ttttatcgga
     1021 tggcggattg agacctcttt tttctcatta gcgtacagac ttgatgaaca agaactgatt
     1081 ttgtgctctt ttgaagcacg taaccaaaca gggcttaacg gccctgtttt atcactgact
     1141 cgcttgctcg aagagttgta ccaccatttt tcgggtatta agaaaatcag tgcgatgaaa
     1201 tctaagattg gttcagattc agaacgtcaa aagcgcgaag agttgtttaa ttacttcatc
     1261 agaaagggcg ctgtgcagca agaaacagaa gacggaattt ggttcgtaat gaatgtaaat
     1321 agttagaaat ttttatttct attaactatt gaggaaaatt taatgaattt atctgaaatt
     1381 actcaacaaa tgggtgaagt aggtaaaacg ctgagtgatt ctgtgccaga gttacttaat
     1441 agcaccgatt tggttaatga ccctgaaaaa atgttagagc tgcagttcgc ggttcagcaa
     1501 tattctgctt atgttaacgt agaaagtgga atgctgaaaa cgataaaaga tttggtctca
     1561 accatttcca accgtagctt ttaattttta tggttaatga tgtttctgcc aacaagatac
     1621 tggtgtgggc ggctgtagct gcggcaaatc ataagcttcc caaatatgca gaatctatcc
     1681 tgaatgtatt gccgcaaatt atacccgata aaaaagatat cgcacattta gaatttatta
     1741 ttttatatgg attaaataga aaaaatgatg cggtaaaggc tctggaggac tttatggatg
     1801 atgagacaag ccagttgtta tgcagtctgg tccacgagaa taatagaagc tggacactaa
     1861 tgttttaatc tcatattgag tttaaacaat tgataaattt cattttggta tgctataaat
     1921 tttaacagag at
//
LOCUS       DAZGLJ010000399         1904 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000399 DAZGLJ010000000
VERSION     DAZGLJ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.408"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..614)
                     /gene="iha"
                     /locus_tag="ACHK49_005024"
     CDS             complement(<1..614)
                     /gene="iha"
                     /locus_tag="ACHK49_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO9265480.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRG"
     gene            553..735
                     /locus_tag="ACHK49_005025"
     CDS             553..735
                     /locus_tag="ACHK49_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265481.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICFLQKVMIIDKTKYK"
     gene            1394..1894
                     /locus_tag="ACHK49_005026"
                     /pseudo
     CDS             1394..1894
                     /locus_tag="ACHK49_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535762.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT          536 a          425 c          402 g          541 t
ORIGIN      
        1 ccgcgtacct gcaggctgac agaatcatcc acaaggggac cactgctcca gaaattaaac
       61 tggctgctgt taccccattt gttgctttcc tgcagattca gccctgcatt gacggaagag
      121 agccatttgt ctgcattctt tctggtaatg atattcacca caccgcccat cgcatcagag
      181 ccatacagtg tggacatcgg ccccctgata acctcaatac gctcaatggc ggccagaggg
      241 ggcatgaacc cggtattcat ggcagaaaaa ccgttgggag tcacgtcact gcttccgccc
      301 tgacgaacac catcaatcag tatcagcgtg taactggctg gcattcctcg gatgctgatt
      361 tccagccctc cggttttacc cgtaccactt tcaacatcca caccctctac tgatctcaga
      421 gcctccgcca gatcgtggta ctggctggac tgcaattcct cctggctaat cacagaaaca
      481 ctggcggctg cgttagtcag ctttttctca tagccggatg ccgagacaat catcacatcc
      541 tctgcagcag ctatgctgct ggctgaaaat ccgagacagg gaatgactac ggaagccaga
      601 gtggttattc gcatattact aatctccgat catgttaacc ggcaagacaa tacacaaata
      661 caaacaataa tgagaataat ttatatttgc tttttgcaaa aagtaatgat tattgacaaa
      721 actaaatata aataacgact gcctcggcat gccagttaac agctatccgt atggcgtgtt
      781 cggttttgcg aataaaaaat ggttactgta gaagcggatt tccaccacat gtcaaccatg
      841 aatggtcagt gatgcgagac gactgggatg atttggtcga tgacttcttc cggcgaatac
      901 tgatagaaac attgaaatat tgtaagagaa agcgttcaaa attctgtggc acgtgaaaaa
      961 ttctacagcg tcatcacctt attcagtcga ccttcaaaat aaacgtttaa cagagacaat
     1021 caccatttgg attgtccgct gaggaggcga acaatttcct agacagcttt atcatgtcag
     1081 cccacattgt tggcgctggc aatactaagg acaaacgatg gggaagtagc tttttagcgt
     1141 tattttcggg agtaaaaaac gtggtatctt tccgtatgtc agttgtatct tgctggtatt
     1201 cgtagtcgtg ataaggcata caacgatatt tatcttgacg ttagtgaaga ataagttgtt
     1261 ttgtgatcta tgttaatctg ggattacaat ccgggcttga tacatcgcat cacgactttt
     1321 attctcttca gaatagaata tgttactccc cgctttacag gctcaggatt tgaattacaa
     1381 attatacttt taagtgaaca ataaccaagg gtatttctta tttcctgaaa ccaggcttgg
     1441 cccatttcgg gatgagtttg aaaaaactct tgacgcatac gatagtgatg aaataaacga
     1501 aacaaattac gtcaacaaac tcaggcgtct tgcctgacat gctcctgaca tcattgatat
     1561 tcacgctcat ctggcatatg catttgcgct ctttatgctg caattctggc gaatgttcat
     1621 ttacagcgtc atcaggatgc cgttatactc attgataaaa tactaacata taaccctgag
     1681 aataatcagg gaacacgctg gttactgggg gcggaattgt tacgtgctgg caagcatggc
     1741 agggctctgt acgtattgac aggacatgtc aatgaatttc ccccctactg gtacgagctt
     1801 gctcttttac attttatgaa tgaggcatat attaatgctg caacggcatt tcgatatgga
     1861 tttttaacaa gtatatattg ttgaaatgct ttgttagaat cttt
//
LOCUS       DAZGLJ010000400         1868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000400 DAZGLJ010000000
VERSION     DAZGLJ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.410"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            210..404
                     /locus_tag="ACHK49_005027"
     CDS             210..404
                     /locus_tag="ACHK49_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265482.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            412..1824
                     /gene="pykF"
                     /locus_tag="ACHK49_005028"
     CDS             412..1824
                     /gene="pykF"
                     /locus_tag="ACHK49_005028"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO9265483.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          503 a          459 c          437 g          469 t
ORIGIN      
        1 ttcacttatc ctcacactaa caactttggc accagacgtt gcgcaaacag cgaagttttt
       61 gcgtaacctt ttccctggaa cgttaaatct ttgataacaa tttattgtct aacaagttgt
      121 atattttttg aaacgctgtt tttgttttcc ttttggatta atttcagcgt ataatgcgcg
      181 ccaattgact cttgaatggt ttcagcactt tggactgtag aactcaacga ctcaaaaaca
      241 ggcactcacg ttgggctgag acacaagcac acattcctct gcacgctttt tcgatgtcac
      301 ctatccttag agcgaggcac caccactttc gtaataccgg attcgctttc cggcagtgcg
      361 cccagaaagc aagtttctcc catccttctc aacttaaaga ctaagactgt catgaaaaag
      421 accaaaattg tttgcaccat cggaccgaaa accgaatctg aagagatgtt agctaaaatg
      481 ctggacgctg gcatgaacgt tatgcgtctg aacttctctc atggtgacta tgcagaacac
      541 ggtcagcgca ttcagaatct gcgcaacgtg atgagcaaaa ctggtaaaac cgccgctatc
      601 ctgcttgata ccaaaggtcc ggaaatccgc accatgaaac tggaaggcgg taacgacgtt
      661 tctctgaaag ctggtcagac ctttactttc accactgata aatctgttat cggcaacagc
      721 gaaatggttg cggtaacgta tgaaggtttc actactgacc tgtcagttgg caacaccgta
      781 ctggttgacg atggtctgat cggtatggaa gttaccgcca ttgaaggtaa caaagttatc
      841 tgtaaagtgc tgaacaacgg tgacctgggc gaaaacaaag gtgtgaacct gcctggcgtt
      901 tccattgctc tgccagcact ggctgaaaaa gacaaacagg acctgatctt tggttgcgaa
      961 caaggcgtag actttgttgc tgcttccttt attcgtaagc gttctgacgt tatcgaaatc
     1021 cgtgagcacc tgaaagcgca cggcggcgaa aacatccaca tcatctccaa aatcgaaaac
     1081 caggaaggcc tcaacaactt cgacgaaatc ctcgaagcct ctgacggcat catggttgcg
     1141 cgtggcgacc tgggtgtaga aatcccggta gaagaagtta tcttcgccca gaagatgatg
     1201 atcgaaaaat gtatccgtgc acgtaaagtc gttatcactg cgacccagat gctggattcc
     1261 atgatcaaaa acccacgccc gactcgcgca gaagccggtg acgttgcaaa cgccatcctc
     1321 gacggtactg atgcagtgat gctgtctggt gaatccgcaa aaggtaaata cccgctggaa
     1381 gcggtttcta tcatggcgac catttgcgaa cgtaccgacc gcgtgatgaa cagccgtctc
     1441 gagttcaaca atgacaaccg taaactgcgc attaccgaag cggtatgccg tggtgccgtt
     1501 gaaactgctg aaaaactgga tgctccgctg atcgtggttg ctactcaggg cggtaaatct
     1561 gctcgcgcag tacgtaaata cttcccggat gccaccatcc tggcactgac caccaacgaa
     1621 aaaacggctc atcagttggt actgagcaaa ggcgttgtgc cgcagctggt taaagagatc
     1681 acttctactg atgatttcta ccgtctgggt aaagaactgg ctctgcagag cggtctggca
     1741 cacaaaggtg acgttgtagt tatggtttct ggtgcactgg taccgagcgg cactactaac
     1801 accgcatctg ttcacgtcct gtaatattgc ttttgtgaat taatttgtat atcgaagcgc
     1861 cctgatgg
//
LOCUS       DAZGLJ010000401         1855 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000401 DAZGLJ010000000
VERSION     DAZGLJ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.411"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            94..396
                     /locus_tag="ACHK49_005029"
     CDS             94..396
                     /locus_tag="ACHK49_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepZ/EspZ family type III secretion system
                     protein"
                     /protein_id="HFO9265484.1"
                     /translation="MDAANLSPSGGVLPLAATINGNVSVDENTGVMIPENGVSRNIRI
                     AAGIALATTALAALGTGIAMACTTDASSQEFLGLGIATGVLGGVTALGGGLAMKYA"
     gene            480..857
                     /gene="sctI"
                     /locus_tag="ACHK49_005030"
     CDS             480..857
                     /gene="sctI"
                     /locus_tag="ACHK49_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFO9265485.1"
                     /translation="MNNINQSENINIHFNKVSQTNFVDDHTPLAPTPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNTDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            859..1431
                     /gene="escJ"
                     /locus_tag="ACHK49_005031"
     CDS             859..1431
                     /gene="escJ"
                     /locus_tag="ACHK49_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFO9265486.1"
                     /translation="MRKYIKNTFLFVFICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNVTISVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            1437..>1855
                     /gene="sepD"
                     /locus_tag="ACHK49_005032"
     CDS             1437..>1855
                     /gene="sepD"
                     /locus_tag="ACHK49_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFO9265487.1"
                     /translation="MNNNNGIVKNDSDWLTALDFVKDVKGSPKHLTFHIYQKNAFLHD
                     FGSYWVLYIELNGDFRQVPTDTFIRLCNILAVSKEYKQMGIFQSNKKWYLCQLFNKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQASTSDTTMPP"
BASE COUNT          638 a          344 c          351 g          522 t
ORIGIN      
        1 tgcaactatt taaataatat aatagttgcc catgggataa tttggttatt gataaccaga
       61 caaatgtcta aattagacaa tggagaaaag ttaatggatg cagcaaattt aagtccttct
      121 ggcggagtat taccactggc agccacaatc aatggaaatg tttcggtgga tgaaaataca
      181 ggagtaatga taccagaaaa tggggtaagt cgtaatatca gaattgcagc cggaattgca
      241 ctcgctacta cagctctggc tgctttaggt actggtatcg caatggcatg cacaacagat
      301 gcaagctccc aagaattctt aggtttaggt attgctaccg gtgtattagg tggtgttact
      361 gcgcttggcg gcggattagc gatgaaatat gcctaatcat cgcatcgcat aaataaaata
      421 gagaggtcat ggatgcatta tgccattacc tctctcatga aaagagacta accgtaaata
      481 tgaacaatat taatcaatca gaaaatatta atattcattt taataaagta tcccaaacaa
      541 atttcgttga tgaccatacc ccgttagcac caacgccctc tgcggctgga gcggctcagt
      601 ttcttgatca attacttcct aaaacagctg gtgtatcttc tccagagcaa gttttaattg
      661 aagaaattaa aaagagacat cttgcaacaa tgaacaccga tctcagtttc gatgccctat
      721 ctgcaggtgg actctcgcca gaagatgtgc tcacattaca aaagaatgtg ctcaacgcaa
      781 acgtcaatgt tgatgtcgta tccaagttag caagtcttct ttcaacatcg gttacaaaac
      841 tggtttcaat gcaataaaat gagaaaatac attaaaaaca cctttttatt tgtttttatt
      901 tgtttgacag ttgcatgtaa agagcaattg tatacaggat taacggaaaa agaagccaat
      961 cagatgcaag cattgttgtt atccaatgat gtcaatgtat ctaaagaaat ggacaaaagt
     1021 ggaaatgtaa caataagtgt ggaaaaagag gattttgtcc gtgcgataac cattttaaat
     1081 aacaatggct tcccgaaaaa gaaatttgca gatattgaag ttatttttcc gcccagtcag
     1141 ctagtggcat cgccatcaca agaaaatgca aaaatcaact atctcaaaga acaagatata
     1201 gaaaggttac taagtaaaat ccccggtgtt atcgattgca gcgtttcatt aaatgttaac
     1261 aataacgaga gccaaccatc ctcagcggct gttttagtca ttagctcgcc agaggttaac
     1321 ttagcaccat cagtcattca gataaaaaac ttagttaaga atagcgtaga cgatctcaaa
     1381 ctagaaaata ttagtgtcgt tatcaagtca tcctcaggac aggacgggta atatgtatga
     1441 acaataataa tggcattgtt aagaatgata gtgactggct aacggctctt gattttgtaa
     1501 aagatgtcaa aggttcacca aagcatctta cttttcatat ctatcaaaaa aatgccttct
     1561 tacacgactt tggaagttac tgggttctgt atatcgagct taatggcgat tttcgccagg
     1621 ttccaacaga taccttcatc agactctgta atattctggc cgttagtaaa gaatataaac
     1681 aaatgggcat ttttcaatct aataaaaaat ggtatctttg ccaactgttt aataaagata
     1741 ataatcatcg cgcaaacatg tcaaaagcaa taatgcaaca cacactcgcc tctttgcttg
     1801 acaagcaatt tgataagtta gagcaagcat ctacatcaga tacaacgatg cctcc
//
LOCUS       DAZGLJ010000402         1843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000402 DAZGLJ010000000
VERSION     DAZGLJ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.412"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..375
                     /locus_tag="ACHK49_005033"
     CDS             <1..375
                     /locus_tag="ACHK49_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9265488.1"
                     /translation="GAIMVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVERNIKVPL
                     TEPQKAGIASFCPYNIGPGKCFPSTFYKRLNAGDRRGACEAIRWWIKDGGRDCRIRSN
                     NCYGQVSRRDQESALACWGIDR"
     gene            650..1219
                     /locus_tag="ACHK49_005034"
     CDS             650..1219
                     /locus_tag="ACHK49_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO9265489.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRGKVIQYQEEC
                     DDVLYEYWTKGFVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKSAHVS
                     KIRTLINEANLLIDFVLADTDKGKITKAD"
     gene            1219..1368
                     /locus_tag="ACHK49_005035"
     CDS             1219..1368
                     /locus_tag="ACHK49_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265490.1"
                     /translation="MEWWLMISDKLITLAKILCVIVGISFLVILVAIFFSTAWRVLTL
                     SGLVG"
     gene            1596..1781
                     /locus_tag="ACHK49_005036"
     CDS             1596..1781
                     /locus_tag="ACHK49_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO9265491.1"
                     /translation="MRELKMKLCVLMLPLVVSACASTPTVQAPCVKPPSPPAWIMQPV
                     PDWQKPLNGIISSSENG"
BASE COUNT          495 a          400 c          509 g          439 t
ORIGIN      
        1 ggcgccatca tggtggatgg taagcctgtg attcctggca tgaagctgtc gaaggaaaaa
       61 tgcgaccggg ttaacgctat cgaacgggat aaggcgctgg catgggtgga gcgtaatata
      121 aaagttccac tgaccgagcc acaaaaagca ggtatcgcgt cattttgccc ctataacatt
      181 ggccccggta agtgtttccc gtcgacgttt tataagcggc ttaatgcagg cgatcgcagg
      241 ggagcgtgtg aggcgattcg ctggtggatt aaggacggtg gcagagactg ccgtatccgt
      301 tcaaacaact gttacggtca ggtatcaaga cgtgaccagg agagtgcgct ggcatgctgg
      361 ggtatcgaca ggtaagcaga atattttgct gaaaaatgac gttggccaac gcgggtagac
      421 aacacgaaat cctgcgaact ggcaaaatgt aagtgaataa agtcaacaag attgtttcat
      481 gaagaggcac cgtaatggtg cctttgtcat ttctgcgctt cgcacaagcg taaataaacc
      541 aaagaacctt tcaggatgag ccctggtgga taaccggcag tggtctggtt aaccctcttt
      601 gggctggtta ttcctgtgcg cagggttcat cactaaaagg aaataaccga tgaatatgat
      661 gaccgtgccg tttcacggcg attctcttta tgtggttaac cataacggcg aaccatacgt
      721 tcccatgaaa cctgtcgttg cggggatggg gctggcctgg caatcacagt tggctaagtt
      781 aagacagcgt tttgcgtcaa ctataacgga aatcgttatg gttgctgagg atgggaaacg
      841 acgcaatatg gtgtccctgc cgcttcgaaa acttgcaggc tggttacaaa ccatcaatcc
      901 caacaaagta aaacccgaaa tccgcggcaa ggtaatccag tatcaggaag agtgtgacga
      961 tgttctctat gaatactgga cgaagggttt tgtcgttaat ccccgtcgaa tgagtgtgat
     1021 ggaagaactc aatcaggctt gcgctgacat gaaacgggat aaaaacattg ccagtgtgtt
     1081 tgctaccggg ctgaatgagt ggaaacaggt taaatccgcg catgtatcaa aaatccgcac
     1141 attgataaac gaagcgaatc tgctgattga ttttgtcctg gctgatacag acaaagggaa
     1201 aataacaaag gcggattgat ggagtggtgg ctaatgatat cggataaact cataacgctg
     1261 gcgaagatcc tctgtgtaat cgtcggcatt tcatttttag tcattctggt tgccattttc
     1321 ttttccaccg cttggcgagt cctgacgtta tcgggactgg tggggtgaaa gagagatgaa
     1381 ccgtgttctg tgtgtggtga ttattgtcct gctggtagcc tgtggtgcac ttagtctggg
     1441 gctgaatcat taccgcgata acgccataac ctacaaagag cagcgcgata aaaaagtcag
     1501 tgagctggag ctggcaaatg caaccattac tgatatgcag cagcgccagc gtgatgttgc
     1561 tgcacttgat gccagatact cgagggaatt agccgatgcg agagctgaaa atgaaactct
     1621 gcgtgctgat gttgccgctg gtcgtaagcg cctgcgcatc aacgccaact gtccaggctc
     1681 cttgcgtaaa gcccccatca cctccggcgt ggataatgca accggtcccc gactggcaga
     1741 agccgctgaa cgggattatt tcatcctcag agaacggctg atggcaatgc agaagcaact
     1801 ggaaggagca caggaatata tccgtaccca gtgtataccg tga
//
LOCUS       DAZGLJ010000403         1840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000403 DAZGLJ010000000
VERSION     DAZGLJ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.413"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(115..1800)
                     /locus_tag="ACHK49_005037"
     CDS             complement(115..1800)
                     /locus_tag="ACHK49_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO9265492.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQSGVWNLADDICDISAVL"
BASE COUNT          532 a          402 c          391 g          515 t
ORIGIN      
        1 tacatgtttt tgtcatcgac ggcctgcgcc acatcagcgc cacataaaat gacgccagga
       61 ataagaactc gcttcattta tttatatctc cgtacagcag ggcttttatt gtttttataa
      121 aaccgcgcta atgtcacaaa tatcatctgc caggttccag acaccggatt gatgtgtaac
      181 cataatgacg ccgcttgtgg gtagtttttc acgcactaaa cgcagtaagc ggatagcctc
      241 ttgttcctca agatgagagg tagtttcgtc aagaaatatc catttcggac gtcgtaaaat
      301 taatcgtgcc agggcgatac gttgtttttc gccgctggaa agaatatctc cccagcgatc
      361 gtggtcatga atacgcgcag ccaatttccc cagaccaacc tgatgcagta cttcgctcaa
      421 cgatttatcg tctacgggca ggggaagtgc tttacaaata atctctttca gtaagccggt
      481 tttgattaac ggtgtttgtg acacatacca actgtcagca ggagaagaaa tatcaccttt
      541 aaaccacggc cagcagtgag ataatgtttt aagcagtgtg gtttttcccg caccagagta
      601 acctttcagt aatagccatt tgcctggcga aacatgaaag ttcaggttct ctaatatgat
      661 cttattatca ggagtacgaa tactcgcgtc agccacttgt accgcatgtt ggcaattttt
      721 aggtttattc gtagggcgct gttcggtgag ttgatggaac tcatacaaac gatcgataac
      781 cgcagccagt tcagcaagtt cgtcatattt ataaataaac cagcttaaat tgttcgatac
      841 cagcataaat gcctggcgcg atttcatcag tccgcccaga ttgatctgcc cgctaataaa
      901 ctgcggcaat aacagaaagt agggaagaac gctaagcgag cgcgaataaa tattctgcca
      961 gtaatcaagc caccgctgac gattcattaa acgatgccag ttctctttaa tggtatgaaa
     1021 attgtcgctc aactcctggc gttgtagaga ctcagcattg cttagcgcaa tcagttctgc
     1081 ctgcttatta tgctgcacaa gatttgttcg aaatgtcgct tcgctacgtt gtttttccac
     1141 attaagcgga cgaatacgtt taccaacctt atgggtaaat aaagttccac cgatcacaat
     1201 gagcacaacg gtatagacca tatacccctg gatattccat tctgttccac caacagtgaa
     1261 tgagagcgta cccgcgcttt gccaaagaat aacggtaaag gtgatcagca tgctaagtga
     1321 ctggatgaag ccaaatgaaa ggctgagcgt tttgctgatc agtaagagaa tgtcttcagc
     1381 gatacgttgg tcagggttat ctgtattttt atgctcgccg tagatctgcg tgaagtagta
     1441 gtttttatct gcaaaccacc gattaaggta gtaatctgtt aaccattcgc gccagcgaat
     1501 agtcagtagt ttaattaacc atgttttgtt tacggatatt aatacaaaga tccccagcag
     1561 ggcagggaac caaaggacaa gctgccaaag cttatcggtt tctttctggc ttagcgcatt
     1621 gaaaaaatca ttattccagt cgtttaacca gacctgaatt ttaaccacgc caaggatcat
     1681 ggcgaggata ataatgatta acaacactga agttttatta ttcttacgca gccaaaaggg
     1741 cttcagcaga cagagatact ttgctatcaa catacaaaac gtaatgggaa tggttatcat
     1801 tagcgaaaat tgattgtgcc atccaatgat tatctatggc
//
LOCUS       DAZGLJ010000404         1838 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000404 DAZGLJ010000000
VERSION     DAZGLJ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.414"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            81..335
                     /gene="bssS"
                     /locus_tag="ACHK49_005038"
     CDS             81..335
                     /gene="bssS"
                     /locus_tag="ACHK49_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO9265493.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSELEGT
                     KVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            450..1568
                     /gene="solA"
                     /locus_tag="ACHK49_005039"
     CDS             450..1568
                     /gene="solA"
                     /locus_tag="ACHK49_005039"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO9265494.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTVIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1616..1729
                     /locus_tag="ACHK49_005040"
     CDS             1616..1729
                     /locus_tag="ACHK49_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO9265495.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          471 a          443 c          443 g          481 t
ORIGIN      
        1 tggtatactg aagttgcttc agataaaaac gcattgaacc tcgaataacg ttgtctagta
       61 acacgaatta gggggccatg atggaaaaaa ataatgaagt cattcagact catccgctcg
      121 tagggtggga catcagcacc gttgatagct atgatgcgct gatgttgcgt ttgcactacc
      181 agaccccaaa taagtccgag ctggaaggga ctaaagttgg tcagacgctc tggttaacca
      241 ctgatgttgc cagacaattt atttcgatat tagaagcagg aatcgccaaa attgaatccg
      301 gtgatttccc ggtaaacgag tatcggcgtc attaacatga catgaccatc cacccaaaag
      361 gcacctcacc ggtgccttta ccatttctgg gttgtatatc tgcattcagt taattactct
      421 gcttaaacca tgagagcaga gagaaaaaaa tgaaatacga tctcatcatt attggcagcg
      481 gttccgtagg cgctgccgcc gggtattatg caacccgcgc cggtttaaac gtgctaatga
      541 ccgacgccca tatgccaccg catcaacacg gcagccacca tggcgatacg cgattaattc
      601 gccatgctta tggtgaaggc gaaaagtatg tcccgctggt cctccgcgcg caaacgctgt
      661 gggatgaact ttcccgccac aacgaagatg atcccatttt tgtacgctct ggtgtcatta
      721 acctcggccc ggctgactcc gcatttctcg ccaacgtcgc ccacagcgcc gaacagtggc
      781 aactcaacgt tgaaaagctc gatgcgcaag ggattatggc ccgctggcca gaaatacgcg
      841 tcccggacaa ctacatcggc ttatttgaga ctgattccgg ttttttgcgc agcgaactgg
      901 cgattaaaac ctggatccaa ctggcgaagg aagcgggctg tgcgcaactg ttcaactgcc
      961 cggtcaccgt aattcgtcat gacgatgatg gcgtaactat tgaaacggtt gacggtgagt
     1021 atcaggcgaa aaaagcgatt gtctgcgcgg gaacatgggt aaaagacctg ctcccggagc
     1081 tgcctgtcca gcccgtacgt aaagtatttg cctggtatca ggccgatggc cgctatagcg
     1141 tgaagaataa attcccggcg tttaccggtg aactgcccaa tggcgatcaa tattatggtt
     1201 ttccggcaga aaacgacgcg ttgaagattg gcaaacataa cggaggccag gttatccatt
     1261 cagcggatga acgtgttccg tttgcggaag tggtcagcga tggttcggaa gccttcccgt
     1321 tcttgcgcaa tgtattgccg ggtatcggtt gctgcctgta cggcgctgcc tgcacctatg
     1381 ataattcgcc tgacgaagat tttattatcg ataccctacc cggccacgat aatacactgc
     1441 tcattaccgg cctgagtggg cacggtttta aatttgcgtc agttttaggg gaaatagctg
     1501 ccgattttgc gcaagacaaa aaaagcgatt ttgatttgac gccattcagg ctttcccgct
     1561 tccaataatc atcattacgg cctccagaat gaggccgttt ttacaggagt atttaatgcg
     1621 tcggctgttg cactatctca tcaataatat tcgcgagcat ctgatgcttt atctttttct
     1681 ctggggattg ttggcaatta tggatctgat ctatgtgttt tatttttagt tttccgcact
     1741 gacataaact gacaaatatt taatctccct ttcatttggc gtgaaaaatt gaatgagatt
     1801 attcctgctt ctacagcact caccagacat ctctgatg
//
LOCUS       DAZGLJ010000405         1824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000405 DAZGLJ010000000
VERSION     DAZGLJ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.415"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            591..881
                     /locus_tag="ACHK49_005041"
     CDS             591..881
                     /locus_tag="ACHK49_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO9265496.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
     gene            905..1156
                     /locus_tag="ACHK49_005042"
     CDS             905..1156
                     /locus_tag="ACHK49_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO9265497.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
     gene            complement(1204..>1824)
                     /gene="traT"
                     /locus_tag="ACHK49_005043"
     CDS             complement(1204..>1824)
                     /gene="traT"
                     /locus_tag="ACHK49_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO9265498.1"
                     /translation="QYTSTIWLEPASERTVFLQIKNTSDKDMSGLQGKIADAVKARGY
                     QIMTSPDKAYYWIQANVLKADKMDLRESQGWLSRGYEGAAVGAALGGGITAYNTGSAG
                     TTLGVGLATGLIGMAADAMVEDINYTMITDVQIAERTRTSVRTDNVAALRQGTSGSKI
                     QTSTETGNQHKYQTRVVSSANQVNLKFEEAKPHLEDQLAKSIANIL"
BASE COUNT          466 a          377 c          429 g          552 t
ORIGIN      
        1 gaattaaaaa acataacgaa attttgcctc attctgcatt tcattgaaat agtatgtttc
       61 tcatcgaaag caagtttgtc aatataaatt tgtaacagaa atgactttcc ggggataaac
      121 aaaactgttc atggcaatgg ctttctctgt cgccaacatg gatacattgt tccggataaa
      181 gcactgatgt cattgactga cgcagactat caggccatct gatgctttct tttccttcac
      241 cggtattgat gatatgcctt atgaaggggg acttattcct gtaacccaat cacaggcact
      301 gtgattactg gtttatgatt gatgctgatt tgagcagata tgcacagcag ttgtggattc
      361 aggggatatt ttctgccaga tattcatacc agattgatga cctctttgct cagatgctct
      421 tcgttctgag ccagccagga ttttgtggcc acatacattg ttcgccgatg gggaatcgag
      481 acaggcgatt ggctgtgatg cgattttgcc gctaactgag gatgagtccg cccctcccgg
      541 ggaattcctg taagttaagc cgtacctgtt gattagcact aaactgggta atgaatttat
      601 ctgccagtat gaatgatgcc ggggacggtt ttgaactcct gatgtcgggt aatatgcagg
      661 attttaatat ttgttgtcgt cgggataatc attatcctga acataaactg atacctttat
      721 cagagttatt tgacgttaat tatggactga atcttgagct gaataaactg gagaaagatt
      781 cttcaggaat taattttgtt tcaagaacgt ccaaaaataa tggcgtctcc gccagggtta
      841 aattaaaaga tggtattagt ccatgccagc agctgtgtta actgtagcag caggaggttc
      901 tgttatggaa tcgtttgtac aggattcacc attttatagt ggaagagatc tttattggtt
      961 acgtcctaaa gttgaactga ctctggaaga gaaattgtac tactgctctt gtataagaag
     1021 aaacagacac aaatacagct acgggcgtca ggcaaaccga acgctgaaga atttactggt
     1081 accgtcatta gattctgttc cggcatgggt atatggtgtg acagggaaaa taatatctga
     1141 attgtctgaa cgttgagact aagagggccg gaggccaggt gaatggccac cgggatattt
     1201 atatcagagt atattggcga ttgattttgc cagctggtct tccagatgag gtttagcttc
     1261 ttcaaacttc agattaacct ggttggcact ggatacaacc cgggtctggt atttatgctg
     1321 gttgccggtt tctgtgcttg tctgaatttt ggagcctgat gttccctgac gcaaagctgc
     1381 aacattatcc gttctgacgc tggttctggt tcgctcggca atctgaacat ctgtaatcat
     1441 tgtgtaattt atatcttcca ccattgcatc cgcggccatg ccaatcagac cggtagccag
     1501 tccaacaccg agagtggtac ctgccgaccc ggtattgtac gctgtgattc cacctccaag
     1561 agcagctccg actgctgcac cttcataacc gcgactcagc catccctgag attcacgcag
     1621 atccattttg tctgctttga gaacgttagc ctggatccag tagtaagctt tatccgggga
     1681 agtcatgatc tgataacctc tggctttgac tgcatcggca atttttccct gtagtccact
     1741 catgtcttta tccgacgtgt ttttgatctg caggaaaacg gtgcgttcgc tcgcaggttc
     1801 aagccagatg gttgaggtgt actg
//
LOCUS       DAZGLJ010000406         1821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000406 DAZGLJ010000000
VERSION     DAZGLJ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.416"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1036
                     /gene="escC"
                     /locus_tag="ACHK49_005044"
     CDS             <1..1036
                     /gene="escC"
                     /locus_tag="ACHK49_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFO9265499.1"
                     /translation="EAQSKAKNKDVVRVFKLNYASATDITYKYRDQNVVVPGVVSILK
                     TMASNGSLPSTGKGAVERNGNLFDNSVKISADPRLNAVVVKDREITMDIYRQLISELD
                     IEQRQIEISVSIIDVDANDLQQLGVNWSGTLNAGQGSIAFNSSTAQANLSSSVISNAN
                     NFMIRVNALQQNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGT
                     LLRVTPRILDDNSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSILPEVQNSEMT
                     TEATLSAGESLLLGGFIQDKESSSKDGIPLLSDIPVIGNLFSSTTKQKHSVVRLFLIK
                     ATPIKSASSE"
     gene            1050..1505
                     /gene="cesD"
                     /locus_tag="ACHK49_005045"
     CDS             1050..1505
                     /gene="cesD"
                     /locus_tag="ACHK49_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFO9265500.1"
                     /translation="MSRKFSSLQDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNRKMAKKAFKACLMWCNEQEKYATYKENIKKLLGNTD"
BASE COUNT          597 a          350 c          364 g          510 t
ORIGIN      
        1 agaagcacag tctaaagcaa aaaataaaga tgtcgttaga gtatttaaat taaactatgc
       61 atcagcgaca gatataacgt ataaataccg cgaccaaaat gttgtcgtgc ctggcgtcgt
      121 ctccatcctg aagacgatgg caagtaatgg ctcacttccc agcacaggca aaggtgctgt
      181 agaaagaaac ggcaacctgt ttgataacag tgtaaagatt tccgcagatc cacgtcttaa
      241 tgcagttgtt gtaaaagatc gtgaaataac tatggacata tatcggcaat taatttctga
      301 gcttgatatt gagcaaaggc aaattgagat ttctgtttca attattgatg ttgatgcaaa
      361 tgatcttcag caactgggag taaactggtc cggtacatta aatgcagggc aaggttctat
      421 tgcctttaac agctcaacgg cgcaagctaa tctcagttct tctgtgataa gcaacgccaa
      481 taatttcatg atcagagtga atgcgttaca acagaactcg aaagcaaaaa tactctctca
      541 gccatcaatt ataaccttaa ataatatgca ggcgattctt gataaaaacg taacgtttta
      601 tacaaaagtt tccggagaaa aggtggcatc tctggaaagt attaccagcg gaacattact
      661 gagagtaaca ccacgtatac ttgatgacaa ctcgaatagt cttaccggaa aacgtcggga
      721 acgagttcgt ttgttacttg atattcagga tggcaatcaa tcgactaacc aaagtaatgc
      781 tcaggatgcc agcagcatct tacctgaagt acaaaattca gaaatgacaa cagaagcgac
      841 attaagcgct ggagaaagtc tgctgcttgg cggatttatc caggataaag agagttcaag
      901 taaagatggt ataccgttgt taagtgatat tcctgttatt ggtaacttat tctccagtac
      961 aacaaaacaa aaacacagcg ttgttcgact ctttttgatt aaagcgaccc cgataaaatc
     1021 agcatctagc gaataataaa tgaggcttta tgagcaggaa atttagctct ctacaggata
     1081 tctatgattt ctaccaggat ggtggtacgt tagcatcact gacaaatttg acgcaacaag
     1141 atctcaatga tcttcattct tatgcctata cagcatatca atctggtgat gttataaccg
     1201 caagaaatct atttcatctg ctaacatatc tggagcactg gaattatgac tacaccctat
     1261 ctctggggtt gtgtcatcag cgtttatcaa atcatgaaga tgcacaactg tgtttcgctc
     1321 gctgtgcaac tttagttatg caagatccca gggcatctta ttattctggg attagctact
     1381 tattggtcgg aaatcggaaa atggccaaaa aagcctttaa ggcttgttta atgtggtgta
     1441 atgaacaaga aaaatacgct acatataaag aaaatattaa aaaattgtta ggtaatacag
     1501 attaaaaact accatctgcc ataccaatcc ccaggctgtc tggggatttt ctatcggaaa
     1561 taataaagct gctttggtga agtgcgccca acgttgagca ccgaccgctg gcagatttat
     1621 ctacaggagt gggttactcc attaaccagg attttatgcg cctgaaccat cttctgctgt
     1681 actcaatgtc atttccgctt atagaataag cagtatatat cttaacttag catcagaaat
     1741 ctgactgttt caatcatcta tagcccagag tgtatttgtc aggaaatgac tttcaaactt
     1801 tggcagagaa aaaggcttat c
//
LOCUS       DAZGLJ010000407         1815 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000407 DAZGLJ010000000
VERSION     DAZGLJ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.417"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..409
                     /locus_tag="ACHK49_005046"
     CDS             <1..409
                     /locus_tag="ACHK49_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9265501.1"
                     /translation="LKQRTKNIALPPHTHQQQNPPQEKTVVSIAVDPESPESFMKRPK
                     RRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNE
                     LHADPLAFEEKHGSQVDLIFRFLDHAFATGVLG"
     gene            422..796
                     /locus_tag="ACHK49_005047"
     CDS             422..796
                     /locus_tag="ACHK49_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9265502.1"
                     /translation="MRIEFVLLYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMLEGLVKK"
     gene            793..1614
                     /locus_tag="ACHK49_005048"
     CDS             793..1614
                     /locus_tag="ACHK49_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO9265503.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNW
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
BASE COUNT          475 a          386 c          508 g          446 t
ORIGIN      
        1 actgaagcag cgcacaaaaa atattgcgct accgcctcac acccaccagc aacagaaccc
       61 accacaggaa aagacggtgg tcagcattgc cgttgatccg gagtctccgg aatccttcat
      121 gaaacgacct aaacgtcgcc gctgggtaaa tgagaaatac acacgctggg taaagacaca
      181 gccgtgtgcg tgttgtggta agccagcgga cgatcctcat catctgattg gtcatggtca
      241 gggtggaatg ggaacaaaat cccacgatat tttcacgcta ccgctgtgtc gggagcatca
      301 caacgagctt catgcggatc cgctggcgtt cgaagaaaag catggttccc aggttgattt
      361 aatttttcgt tttcttgatc acgcctttgc aaccggcgtg ctcgggtaaa agaggttact
      421 gatgcgtata gagtttgttt tgctttaccc gccgacggtg aacacctact ggcgacgtcg
      481 tggcagcaca tattttgtat caaaagccgg tgagcgttat cgccgggctg tggcgcttat
      541 tgttcgccag cagcggctga aattaagcct gtccggaagg ctggcgataa agattattgc
      601 cgagccaccg gataagcgcc gccgtgacct ggacaatatt ctgaaagcgc cgctggatgc
      661 gctgacgcat gcgggagtgt taatggacga tgagcagttt gatgaaatca atatcgttcg
      721 tggtcagcca gtatctggtg gacgtctggg ggtgaagatt taccccataa tgcttgaagg
      781 gctggtcaaa aaatgaaact ggaagattta ccgaaatact actccccaaa atcccccggc
      841 ctgactgatg catcggcctc aacgtcgaaa gatacgctga gtatcactga tgtgatggcc
      901 gcgcagggca tgacacagaa ttgggctgag atggggtttt ctgcgttcct tgggaaaatg
      961 ggcattagta tgaatgacag agagcgggca acagaattgc tgacagaata tgcactcagt
     1021 cggtgtgatc gcgtggcggc gttaagaaaa ctcccggcag aaataaaacc ggcagtgatg
     1081 cgtattatgg cttcgtatgc gtttgaagat tatgcccgta gcgcggcgag caaaaaacag
     1141 tgcccctgtt gtcacggaaa aaaatttatt gaaagcgagg tttttacaaa caagatccag
     1201 tatccggatg gtaagccgcc agtgtgggca aagtgcacaa aaggcgtgta tccgtcttac
     1261 tgggaggaat ggaaaaaagt cagggaggtg gtaaaagttg cctgtccgga gtgtggaggg
     1321 aagggggagg tttccaccgc ctgtaaagat tgtcgtgggc gcggtgttgc cattcatcgt
     1381 gaagagtcgg taaaacgtgg tatgcctgtt atcagagact gccagcgttg tggtggtcgt
     1441 ggctatgaaa gattaccttc aacggaggca tttaatgcca tatgtaatgt aaccgatgcc
     1501 atatctcttg atacatggaa aaaaacagtt aaacgttttt acgatacgct ggtggtgcag
     1561 tttgatattg aagaagcatg ggcagaacaa caactgaaaa aggtgaccag atagctttgt
     1621 tgatttttcc cgaatctgtg gtaaatttgc cctaacgatg ggcgttttat gcctgacgtt
     1681 agaagatttt ttacacccgt cgccaggcgg gtttttttat gactgaaatc acgccagtac
     1741 agtaaacgcg ctggtggttg tgaataccgg tctttcagct tgctggcttt ttcgacaaga
     1801 gttattggtg tgtca
//
LOCUS       DAZGLJ010000408         1798 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000408 DAZGLJ010000000
VERSION     DAZGLJ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.418"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(123..1631)
                     /gene="putP"
                     /locus_tag="ACHK49_005049"
     CDS             complement(123..1631)
                     /gene="putP"
                     /locus_tag="ACHK49_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO9265504.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
BASE COUNT          482 a          556 c          412 g          348 t
ORIGIN      
        1 ggggccgcgg cgattggccg ccccctgcgc gctccctgca ccattcaatt tatatggcga
       61 ggcacattaa gggagcgcaa cccgctgctg aagccaaaca aaaccgccgc aggctaagtc
      121 ccttagcttt cctgcaaccg tgacggcgga gccgaatgat agtgcgcatc ggcctcggca
      181 aagcgttttt gcatcgccgc tgacggcgct ttacccagca aactaaacac tacaatcccg
      241 atactgccga agatgaagcc cggaataatt tcgtagagtc ccagccagcc gaactgcttc
      301 cagacgataa ccgtcagcgc accgatgatc atccccgcca gcgcaccgtt gcgcgtcatg
      361 cgtgaccaca tcaccgagaa cagcaccacc ggaccaaacg ccgcgccaaa gcctgcccac
      421 gcgtagctca ctaagcccag cacgcggttt tccgggtttg ccgccagcgc aatcgccacc
      481 agcgccacca ccagcaccat cacacgtcct acccacacca gctctttctg gctggcctgt
      541 ttacgcagaa acgctttgta taaatcttcg gtaattgcac tggaacatac cagcagctgg
      601 caacttaagg ttgacattac cgccgccaga atcgccgaca gcagaatccc ggcaatccac
      661 gggttaaaca gaatttgcgc cagttcgata aacacgcgct cggcgttctg gtttaccgcg
      721 cctgctaccg ccgggtgctc attaaagtaa gcaatcccaa agaagccaac agccaccgcc
      781 cctgccaggc agaggatcat ccaggtcata ctgatacgac gcgcatggac aatgctgtgg
      841 tgagagtctg ccgccataaa acgcgccagg atgtgcggct gcccgaagta acccagcccc
      901 caacccatca gtgagataat ggcgacaaag ttcagccctt tgagcatatc aacgttttcg
      961 atgctctttt gtttgatcac ttccagcgag tcaccaaagc caccgacact gataatgacg
     1021 ataaccggcg tcaggatcag ggcaaaaatc atcaggctgg cctgtacagt gtcagtccag
     1081 ctcaccgcga ggaaaccgcc aataaaggtg taaaggatcg tcgccgcagc tcccgcccac
     1141 agagccgttt cgtagctcat gccaaaggta ctttcaaaca gacgcgcgcc tgccacaatg
     1201 cccgaagcgc aataaatggt gaagaacagc aaaatcacca gcgcggagat aatgcgcaaa
     1261 atgcggcttt tatcttcaaa gcgcccggtg aaataatccg gcaatgttaa ggcgttattg
     1321 ttgtattcgg tatgcacacg caaccgcccc gccaccagct tccagttgat ccacgcgcct
     1381 aatgtcaggc caatggcgat ccaactttcg gaaatcccgg aaagaaaaac cgcgcccggc
     1441 aaccccatta acagccagcc gctcatatcc gacgcacccg ccgataatgc cgtcacgaat
     1501 ggcccaaggc tacgaccgcc cagaatatag tcgtcaaagt ttttcgttga tcgccaggcg
     1561 ataaacccaa tcaatatcat gccaaagata tagacacaaa atgtcaccaa catcggtgtg
     1621 ctaatagcca tctaaagtct ccaaaaaatt attatcggca atgtcgaaac ttgccgttat
     1681 atctgccacc ggaacggggt aacagagttt atgttttacc agggcgaccg tatcctgccg
     1741 gaagcgctgg ttattcacaa tcgatttaac acaccattta cattaaattt tagtgctc
//
LOCUS       DAZGLJ010000409         1767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000409 DAZGLJ010000000
VERSION     DAZGLJ010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.419"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            122..337
                     /locus_tag="ACHK49_005050"
     CDS             122..337
                     /locus_tag="ACHK49_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO9265505.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGK"
     gene            342..686
                     /locus_tag="ACHK49_005051"
     CDS             342..686
                     /locus_tag="ACHK49_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO9265506.1"
                     /translation="MKKKYELVVKGINNYPDKITVTVALEIGGYPSLLLPDVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPIILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            737..1270
                     /locus_tag="ACHK49_005052"
     CDS             737..1270
                     /locus_tag="ACHK49_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9265507.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1569..>1767
                     /locus_tag="ACHK49_005053"
     CDS             1569..>1767
                     /locus_tag="ACHK49_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFO9265508.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKEQRDKKVSEL
                     ELANATITDMQQRQRDVAALDA"
BASE COUNT          455 a          373 c          517 g          422 t
ORIGIN      
        1 gccgatgcga ctgaacctca acccgaatcg gtcatgatgc tgtaacgggg agtcagggcc
       61 atcagtagtt aactggtggc ttttttattg ttgtcagctt ccggataacg ggagacgggg
      121 tatgtaccag atggaaaaaa tcacaacggg tgtgtcatac accacgtcag cggtggggac
      181 gggatactgg ttactgcagc tgctggacaa agtctctccg tcccagtggg tggcaatcgg
      241 tgtgctgggg agtctgctgt ttggcctgct gacgtatctt actaacctgt atttcaaaat
      301 cagagaggac cgtcgtaagg cggcgcgggg aaagtaaagc gatgaagaaa aaatacgaac
      361 tggttgttaa agggataaat aattacccgg ataagattac tgttactgtg gcactggaaa
      421 ttggtgggta tccgtcactg ttgttgccag atgtggcgat tagtcttgac cgtactgaag
      481 gtgccacgct ggagttttac gaagctgagg cgaaaaagca ggcgaagcag tttttcatgg
      541 atgttgctgc cgggttatgt gaaggggatg gtccgttgcc ggaaaagcgg cccatcattt
      601 tagaggcgca ggatgtgttg ataacctaca gaggaaaact accgggaata attactggtt
      661 ctctgaagac tccaccgctg gcctgaagac ttaatatatc cagggatttg aaatcgataa
      721 accctgataa atatccatga acgcaaaaat cagatacggc ctgtcggctg ccgttctggc
      781 gctgattggt gcaggggcgt ctgcgcctga aatcctcgac cagtttcttg acgaaaaaga
      841 aggtaaccac accacggcat accgtgatgg tgcggggatc tggaccatct gccgcggtgc
      901 catcctggtg gatggtaaac ctgtcgtccc gggcatgaag ttgtcgaagg aaaaatgcga
      961 ccaggttaac gccattgaac gtgataaggc gctggcatgg gtggagaaaa acatcagagt
     1021 gccactgacc gaaccccaga aagcggggat tgcgtcattc tgtccgtaca acattggccc
     1081 cggtaagtgt ttcccgtcga cgttttacag acggattaat gccggtgacc gcaggggagc
     1141 atgcgaggcg attcgctggt ggattaagga cggtggcaga gactgccgta ttcgctcaaa
     1201 caactgctac ggtcaggtct cacggcgtga ccaggagagc gcgctggcgt gctggggaat
     1261 tgacagataa gcagaatatt ttgctgaaaa atgcgatttg ctcacgcgga cggataacac
     1321 gaaatcctgc gaactgacaa aaactaagtg aataaaagta aaaaccccgt ttgttggctg
     1381 caagcggggt tttgtgtttc ctgactccgg aaaagtcaaa ggagaaagtg tgtttgattt
     1441 tagcaaactg attcgggaga ttcgaatgat ggctgaaaaa ttatccacct ggaagttcat
     1501 ccttatctgg ctggtgtttg tgattatggc ttccggttat ttcattggtc agatacgctg
     1561 gtggtgaaat gaaccgcgta ctgtgcgtgg tcatcattgc cctgctggtg gcctgtggtg
     1621 cgcttagtct ggggctgaat cattaccgtg ataacgccat aacctacaaa gagcagcgcg
     1681 ataaaaaagt cagtgagctg gagctggcaa atgcaaccat tactgatatg cagcagcgcc
     1741 agcgtgatgt tgctgcactt gatgcca
//
LOCUS       DAZGLJ010000410         1729 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000410 DAZGLJ010000000
VERSION     DAZGLJ010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.420"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..309
                     /locus_tag="ACHK49_005054"
     CDS             <1..309
                     /locus_tag="ACHK49_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265509.1"
                     /translation="INISWASKPKSNDRETNEYVYDMATFEGISYLGARSPLTQIAEQ
                     IKGIREDWKPIAQGAKKVKADVYTSSDRNEERTYLQEQHDLAIKRRDEKREKRLESGE
                     "
     gene            complement(358..1182)
                     /locus_tag="ACHK49_005055"
     CDS             complement(358..1182)
                     /locus_tag="ACHK49_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiJ-NTD4 domain-containing protein"
                     /protein_id="HFO9265510.1"
                     /translation="MIKPFSVRYGHVDVREHVQLNDLNSDTRMALWNCLYLFLWTNNR
                     QTATATKCAQSVWIYYLNQPADNIPRYESGYKSDKTLLTAIRDYIYGEAWYLVYDLIE
                     FIIERTNSYINLSKHLNSIFKKHGVGYTIINGCITPISNDNEIESVQNAVDNGTDSSR
                     SHFERALQLMTDREQPDYRNSIKESISAIESLCRKITGNDKGTLGACLKAIEEKGYIH
                     SAMKGAFSQLYGYTSDQGGIRHALTEEDVNPTLAEAKFMLVTCSAFSNYLLSKISD"
BASE COUNT          554 a          344 c          348 g          483 t
ORIGIN      
        1 attaatataa gctgggcaag caaacctaaa tcaaacgata gagaaacaaa tgaatatgtg
       61 tatgacatgg cgacatttga aggaataagt tatctaggag cgagaagccc attgacgcaa
      121 attgcagaac aaattaaagg tataagagag gattggaaac ctattgcaca aggagctaaa
      181 aaagtaaaag cagacgtata tacttcaagc gatagaaacg aagaacgcac gtatctgcaa
      241 gagcaacacg atttggcaat aaaaaggaga gatgagaaaa gagaaaaaag attagagtct
      301 ggtgaataat tttaaaggga gtgggtaact aacccactcg taactataaa cctgtaatta
      361 atcacttatt tttgacaaca gataattact gaacgcactg caagtgacta acataaattt
      421 agcttcagct aaggtaggat ttacatcttc ttctgttagc gcatgacgta ttccaccctg
      481 atcacttgta taaccataga gctgactaaa agcgcctttc attgcagagt gtatatatcc
      541 tttttcctct atagctttaa gacaagcccc caaggttcct ttatcattgc ccgtgatttt
      601 cctgcataaa gattcaattg cagagataga ctctttaatc gagtttctgt agtctggctg
      661 ctctctatcc gtcattagtt gtaacgccct ttcgaaatgg ctacgcgatg aatcagtgcc
      721 attatcaact gcgttctgaa cactttcaat ttcgttatca tttgaaatag gagtaataca
      781 accatttatt atggtataac caacgccatg ctttttaaag atggaattga gatgcttcga
      841 tagattaata tatgaattag ttctctcaat gatgaactca attaaatcat ataccaaata
      901 ccatgcttcc ccatatatat aatctcggat agcagtcagc aacgtcttat cacttttgta
      961 tccactttca taacgaggaa tattatccgc aggttgattt agataatata tccacacaga
     1021 ctgcgcacat tttgttgctg tagcagtttg acgattgtta gtccaaagga aaagatataa
     1081 gcaattccac aatgccatgc gtgtatcaga attaagatca ttcagctgga catgctctct
     1141 aacgtcaaca tgaccatacc tcacagaaaa tggctttatc attcttcccc tgcctgattg
     1201 ttatgttcgt ttaaattatg cagtctaagt ttgatacaat catatattaa gcaatagaat
     1261 ccataaagcc ccgttggtgg taagacaaca agcggagcct cttgcgactt catcagggtt
     1321 atgtcagtta gtgaggctta tcaggatgac catgtaccca aggggagcga ggccgaaccg
     1381 ctggcagagc ttgcatatgg tctgcaagta ccgtgaccac cataaacctg tttatcccct
     1441 gcggcgtctc gcgacggcga cagaatgggg gcatcgggct aaaatgcgct gcactaaaag
     1501 ttttgggatt gtttagagac ggtggctacg tcatagcttt caagttgagt cagtacgtgt
     1561 ttgacgcttg gaacgacacg ccgctatgag ttaacatccc aattaccact atcaaccaca
     1621 gcggcttttt caagggacat aacagagcat ttcttacagg caaaagagac agatttaatc
     1681 gtctaactaa ctgtattgta aggaatttag taacttggtg cgaaggccg
//
LOCUS       DAZGLJ010000411         1714 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000411 DAZGLJ010000000
VERSION     DAZGLJ010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.421"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..116)
                     /locus_tag="ACHK49_005056"
     CDS             complement(<1..116)
                     /locus_tag="ACHK49_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO9265511.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLLEA"
     gene            complement(149..448)
                     /locus_tag="ACHK49_005057"
     CDS             complement(149..448)
                     /locus_tag="ACHK49_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO9265512.1"
                     /translation="MEQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDGLTNKKRPVCKTERSEQIQMEF"
     gene            complement(587..820)
                     /locus_tag="ACHK49_005058"
     CDS             complement(587..820)
                     /locus_tag="ACHK49_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9265513.1"
                     /translation="MSNELLRWRKEASSEEWKRLAALANTSVGYLDQIVYGFRRASPD
                     KANAIEEATRNFTGYKPVKKENLVFVSRRASAA"
     gene            934..1638
                     /locus_tag="ACHK49_005059"
     CDS             934..1638
                     /locus_tag="ACHK49_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9265514.1"
                     /translation="MENKDIRKSNLAFLLDEHKKIAGNTNASFADKLGVSPSQLTQVS
                     GEKSTRNIGDKLARKFEAALGLPNGWLDLVHDVTPIASCSDSLTFVGQVRKGLVRVVG
                     EAILGVDGAIEMTEERDGWLNIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKVF
                     PGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILKQ
                     SRHLDDIEAREWLKSS"
BASE COUNT          474 a          354 c          400 g          486 t
ORIGIN      
        1 gcctcaagca gcatatttga tagtctggcg taaccatcat cgagatctgc cacattacgc
       61 tcctgttcgg caaagttacc tctgccgaag ttgagtattt ttgctgtatt tgtcataatg
      121 actcctgtgg attgatccag taattccctc agaattccat ctggatttgt tcagaacgct
      181 cggtcttgca caccgggcgt tttttattgg tgagtccatc aagcgcatac ttaaaagctc
      241 tgctaatcgg actgatgtct gatgccattc cgaaagcaca caagaccgaa gcaataaatc
      301 tccagtccgt tctgcttatc ttcgattcat gacagccaat catctttgcc agaccgcgct
      361 gggtaagcgt tgacaggttg ataagtaaat ctgtttctgc gcgatcaaca tcacgctgtg
      421 atagtttgct gtaacttgtt tgttccattt cttaagattt ccaatagtga atagctagtt
      481 gaaaggtatg cgtggaaacg catatggcct tagttggtca gatatcttgg aactcgcttt
      541 tcagcgacgt aggacgaatg tccgttgtta caaagagcgg ggttacttat gctgctgatg
      601 ctctacgcga tacgaacacc agattttcct ttttcacagg tttataaccc gtgaaattac
      661 gagtggcttc ttcgattgca ttcgctttat ctggggaagc tcttcgaaac ccatatacaa
      721 tctgatcaag atagccaact gaagtattcg ctaatgcggc gagtcgcttc cattcctcac
      781 tagaagcctc ttttcgccag cgtagtagtt cattactcat tagtgcctcc gtttatcaca
      841 cagagcgact ttaccatttt gataaataaa acgcaatgaa gatttatcaa aatgcatatt
      901 tatccatttg ctaaatagag ggagaatcgt gggatggaaa acaaagatat tcgcaaatcg
      961 aatctggcgt ttttgctaga tgagcataaa aaaatcgcgg gtaacactaa tgcaagcttt
     1021 gccgataagc ttggagttag cccttctcaa ctcacgcaag tctccggtga aaaaagcact
     1081 cgaaacatag gggataaact agcaagaaaa ttcgaagccg cacttgggtt acctaatggg
     1141 tggcttgatt tggtacatga tgtaacacca attgcatcat gctcagattc tttaactttt
     1201 gtcggtcagg taagaaaagg gttagtgcgc gtggttggtg aggcaattct tggtgttgat
     1261 ggtgccatcg agatgaccga agagcgcgat gggtggctca atatttatag cgatgatcca
     1321 gatgcctttg gtcttcgtgt gaaaggagac agcatgtggc ccagaataaa atcaggagaa
     1381 tatgtactca ttgagcctaa caccaaagta ttcccggggg atgaggtgtt tgtcagaact
     1441 gttgaaggac acaacatgat caaagttctt ggctatgaca gagatggaga ataccaattt
     1501 acaagcatca accaggacca caggccaata acgttgcctt atcatcaagt agcaaaggtg
     1561 gagtatgtgg ctggtattct gaagcaatct cgccatctgg atgacatcga ggcaagggag
     1621 tggctgaaaa gttcgtgact tcatcgtcac atagctggta accagtggcc tgaagagacg
     1681 tttgggtgat gtgatagaga tcattattca attc
//
LOCUS       DAZGLJ010000412         1710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000412 DAZGLJ010000000
VERSION     DAZGLJ010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.422"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            55..273
                     /gene="ygeI"
                     /locus_tag="ACHK49_005060"
     CDS             55..273
                     /gene="ygeI"
                     /locus_tag="ACHK49_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeI"
                     /protein_id="HFO9265515.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMAPFQNDMYSEDNPISPYYKIEW"
     gene            356..858
                     /locus_tag="ACHK49_005061"
                     /pseudo
     CDS             356..858
                     /locus_tag="ACHK49_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(877..1509)
                     /locus_tag="ACHK49_005062"
                     /pseudo
     CDS             complement(877..1509)
                     /locus_tag="ACHK49_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
BASE COUNT          613 a          311 c          252 g          534 t
ORIGIN      
        1 gttaaaagta tgatgtaaag accattggcc tggacaatta aaaaagggag ctttatgact
       61 aatccaatcg gtatcaataa tttatctcaa agttcaaata tagcgaatgc aacgggtgat
      121 gaagtcgtat cattagataa acacatcaac accagtgcta ctgatacaga tcaaatacaa
      181 gcatttatag tttcaacatg gatggcgcct tttcaaaatg atatgtattc agaagataat
      241 cctatctcac cttactataa aattgaatgg tagacgattg accaaataaa tcataacgag
      301 atacttttat aatagtatct cgtccctttc attcaatcct cacacatgaa aaaatatgcg
      361 ctcaccaaaa gttaaattct taaccatctt cacgttttgt atatttatta caaaaatgtc
      421 tttcgccagc aactcttgct ctaatgaagc aggaacaatg ttcagaatcg aaccaaattt
      481 aattaaagct atcgctcttg ttgaaagcaa tctaaaaaag gatagcatag gcaagaacag
      541 agataagaat aacaatataa agagtttaga ttattggtta atgcagataa accagatgca
      601 tattcccctg ttaaaaaaac gtggaataat aaaagatgaa cgagatttgt tagataaccc
      661 ttgtctgaac attaaaatag gaaccgagat tttatataac catttttcac gttgtggagt
      721 aacgtggcaa tgcctgggga cttataatgc gggttttgca atggataacc aaaaaaagag
      781 acagcaatat gccccaaaat atacattgta tataccaggc ttaatgaact agacaatcgt
      841 aaggcttcag caaaatgagc ctaaagcctc ttttttttat atagtgcaaa cacccatacg
      901 taaagctgtt ttgatcaact cgataccact gtgaacatct aatttcttca tgatattgag
      961 tctatgagtt tcgactgttt ttctgcttaa ctgcaagaaa ttagcgattt ctttgtttgt
     1021 tttaccatca gctatgagtt gtaatatttc tgactcacga tttgtaagtt gattatcaga
     1081 ggaatacctg gaagatattt tttcacaatc catatgtaca ctatcaaaat aagtgtaacc
     1141 attactaatc gaatcaatag cacgatttag tttcgccggg tgactggttt tccatacgca
     1201 tcctttcgca cctgcttcaa gcaattttat tgaatcaata tacggcatct ttgctacaaa
     1261 tgtaataata tgagcatcta tcttatgcga tttaacccat tttacgagtt cggaccccat
     1321 cattccacct gcaaattctc ctaaaaaaaa tatttaggct gtagatttgt tacactccgc
     1381 tatagctttc ttcagatctt tatagccccc aacaacctca tataaatcag gataatgtcc
     1441 gagaaatcca attatcccat caatcataaa cggctgctga tctgaaacta caattttaat
     1501 ttttcccata tattcatcct gagtgtaaat gctttaacgc attcatttat aatcatccct
     1561 tccattatta ttataagcaa aatccaaaga atacattgat gaaataataa tgaaatataa
     1621 ttaaaaataa aatttttgcg taaaaaatac cacaggcatt aaaaaatcat gagatgatta
     1681 aaatattaca attagattat attcaaatca
//
LOCUS       DAZGLJ010000413         1709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000413 DAZGLJ010000000
VERSION     DAZGLJ010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.423"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..610)
                     /gene="nleH1-2"
                     /locus_tag="ACHK49_005063"
     CDS             complement(<1..610)
                     /gene="nleH1-2"
                     /locus_tag="ACHK49_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HFO9265516.1"
                     /translation="MLSPSSINLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDSGTQV
                     TVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEAMLNTSPVSTTI
                     ERGSVHSNRPDLPPVDYAQPELPPADYTQSELPRVSNNKSPVPGNVIGKGGNAVVYED
                     MEDTTKVLKMFTISQSHEEVTSEVRCFNQYYGSGSAEKIYNDN"
     gene            complement(717..788)
                     /locus_tag="ACHK49_005064"
                     /pseudo
     CDS             complement(717..788)
                     /locus_tag="ACHK49_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            782..1709
                     /locus_tag="ACHK49_005065"
                     /pseudo
     CDS             782..1709
                     /locus_tag="ACHK49_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          511 a          421 c          356 g          421 t
ORIGIN      
        1 cattatcatt atatattttc tctgcactcc cggaaccata atactgattg aaacaacgaa
       61 cttcgcttgt cacctcttca tggctttgag atatagtaaa catcttcaac acttttgttg
      121 tatcttccat atcttcatac acgacagcat taccaccttt accaataaca ttacctggca
      181 cgggtgattt attgttgcta accctcggca actctgattg agtataatcc gctggaggta
      241 actccggctg cgcataatcc actggaggta aatcaggtct gttcgaatga acagagcctc
      301 tttctatagt cgtgctcact ggcgatgtat tcagcatagc ctcaattttt ctgctaatct
      361 ctcccttagg ccatcccaac ctgtgcaaca gattagtaaa acaaccacta tgactttctc
      421 ttgtaacgca aaacttatta tcagtgacca caacacgata tgttctgttg ccaaccgtta
      481 cttgcgtccc gctatcagag tgaacagcag catcccttac agaggataaa acacgattat
      541 caggcgaagt caggtttctg gttaaagaat tccatgaaca tcccaaattt atagaagagg
      601 gcgataacat acatttcaac cttcaaaata aacctatcta attattccta acagacatcc
      661 cccatacatg acaacaaaaa ccggagccgg actccggttt ttgtgaagct atcgggttac
      721 ttcatttcgc caatattttc ccacttcccg tcagcacgca ggatttgcag cggtcttact
      781 acacactgac ccgaggacga aaaaagagtt cgggctggga agagacagaa gagtagcaat
      841 ttcagaagca ttcacaccaa cattgagctt ttcagcgaaa gtgggcacga atcattgctg
      901 gacagggtta aaggcactga ctcaatcact cttcatacgt ggcttgatcg atatgaaaca
      961 atcctcagct agagggggag caagccgaaa acattactcg actacgccag caaaatcagg
     1021 gcaattcgaa gaaaattgcc ggacaaaccg ctcactgaca tatcaacgaa agaggtggca
     1081 gcaatgctaa acacctacgt cgcagaaggt aaagcggttt ccgcaagagt aatcaggtca
     1141 acccttgttg acgtttttcg aggggcaata gccgaggggc atgtggcaac gaatccagta
     1201 acaacaaccc gtgcagcaaa gtcagaagta aggcgctcaa ggctgacagc taatgagtat
     1261 gtcgctattt accatgctgc tgagcacctc cccatctggc tgaggctgtc aatggattta
     1321 gctgtcgtta cagggcagag agtgggcgat ttgtgcagaa tgaagtggtc agacataaac
     1381 gatggtcatc ttcacattgg acagagtaaa acaggagcca aaattgccat tccgctggct
     1441 ctaaccattg acgcactcga catctcactg gttgatacac tacagaaatg cagggaggcc
     1501 agcagcagtg aaacaataat cgcatcaacc tatcacgaac cactttctcc agccacagta
     1561 tcacggtatt taacaaaggc gcgaaatgca tccgggatct cgtttgatgg agacccaccg
     1621 acatttcatg aactacgtag tctgtccgcg aggctatatc ggaaccagat tggctacaag
     1681 tttgcacaac gtcttcttgg acataaatc
//
LOCUS       DAZGLJ010000414         1672 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000414 DAZGLJ010000000
VERSION     DAZGLJ010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.424"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1672
                     /locus_tag="ACHK49_005066"
     CDS             <1..>1672
                     /locus_tag="ACHK49_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO9265517.1"
                     /translation="LNDFLGAMTEDDARPEALRRFELMVEEVARNASAVAQNTAAAKK
                     SASDASTSAREAATHATDAAGSARAASTSAGQAASSAQSASSSAGTASTKATEASKSA
                     AAAESSKSAAATSAAAAKTSETNAAASQQSAATSASTATTKASEAATSARDASASKEA
                     AKSSETNASSSASSAASSATAAGNSAKAAKTSETNAKSSETAAGQSASAAAGSKTAAA
                     SSASAASTSAGQASASATAAGKSAESAASSASTATTKAGEATEQASAAASSASAAKTS
                     ETNAKASETSAESSKTAAASSASSAASSASSASASKDEATRQASAAKSSATTASTKAT
                     EAAGSATAAAQSKSTAESAATRAETAAKRAEDIASAVALEDASTTKKGIVQLSSATNS
                     TSESLAATPKAVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSTSEMLAATPKSVKA
                     AYDLANGKYTAQDATTAQKGIVQLSSATNSASETLAETPKAVKAANDNANGRVPSARK
                     VNGKALSADITLTPKDIGTLNSTTMSFSGGAGWFKLATVTMPQASSVVS"
BASE COUNT          456 a          487 c          490 g          239 t
ORIGIN      
        1 cgctgaatga ttttctcggt gccatgacgg aggatgatgc ccgtcctgag gcactgcgcc
       61 gttttgaact gatggtggaa gaggtggcgc gtaacgcgtc cgcggtggca cagaacacgg
      121 cagccgcgaa gaagtcagcc agcgatgcca gcacatcagc ccgtgaggcg gcaacccatg
      181 cgactgatgc tgcaggctca gcacgcgcag ccagcacgtc agccggacag gccgcgtcgt
      241 cggctcagtc agcgtcttcc agcgcaggaa cggcatcaac aaaggccact gaagcatcaa
      301 aaagtgctgc cgctgcagag tcctcaaaaa gcgcggcggc caccagtgcc gctgcggcga
      361 aaacgtcaga aacgaatgcg gcagcgtcac aacaatcagc agccacttct gcatccaccg
      421 cgaccacgaa agcgtcagaa gctgccacct cagcccggga tgcgtcggct tcaaaagaag
      481 cggcaaaatc atcagaaacg aacgcatcat caagcgccag tagtgccgct tcctcggcaa
      541 cggcggcagg aaattccgcg aaggcggcaa aaacgtccga gacgaacgct aagtcttctg
      601 agacggcagc gggacagagc gcctcagctg cggcaggctc aaaaacagcg gctgcgtcgt
      661 ctgccagtgc cgcgtcaaca agtgccgggc aggcctcagc cagtgccacc gccgccggaa
      721 aatcggcaga aagcgccgca tcatccgctt caacagccac aacgaaggct ggcgaagcca
      781 ctgaacaggc cagcgcagca gcgagttctg cttccgcagc gaagacatcc gaaacgaacg
      841 cgaaagcgtc ggaaaccagc gcagaatcct caaaaacggc tgccgcatcg tcagccagtt
      901 cggcggcgtc atcggcatca tcggcgtctg cttcaaaaga tgaggcgacc agacaggcgt
      961 cagcagcgaa gagcagcgcc acgacggcat ccacgaaggc gacagaggca gctggcagtg
     1021 cgacggcggc agctcagagc aaaagtacgg cggaatccgc ggcaacgcgc gccgagacag
     1081 cagctaaacg ggcagaggat attgcatccg ccgtggcgct tgaggatgcg agcacgacga
     1141 aaaaggggat agtacagctc agcagtgcga ctaacagcac ttccgagtca ctggcggcaa
     1201 cgccaaaagc cgttaaggcc gcgtatgacc tggctaacgg gaaatacacc gcacaggatg
     1261 caacgacagc acagaaaggg atcgttcagc tcagcagcgc gaccaacagc acatctgaaa
     1321 tgctggcggc aacgccaaag tcggtaaagg cagcctatga ccttgctaac gggaaatata
     1381 ccgctcagga cgctacgaca gcacaaaaag gaattgtcca gctcagtagt gcaaccaaca
     1441 gcgcatctga aacgcttgcc gagacaccga aagcagtgaa agcagctaat gataatgcga
     1501 atggtcgggt accttctgcc cgtaaggtga atggtaaggc gctttcagcg gatataacac
     1561 tgacgccgaa agatattggt acgcttaact caacaacaat gtcattcagc ggtggtgctg
     1621 gttggttcaa attagcaacg gtaaccatgc cacaggcgag ttctgttgtt tc
//
LOCUS       DAZGLJ010000415         1665 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000415 DAZGLJ010000000
VERSION     DAZGLJ010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.425"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..185
                     /locus_tag="ACHK49_005067"
     CDS             <1..185
                     /locus_tag="ACHK49_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretion protein EspJ"
                     /protein_id="HFO9265518.1"
                     /translation="QKKYGTTENSSWVSASVGSLLDKGAKVYPDISCSLRLGEPFIIT
                     LPETVRLDVNIHPLKK"
     gene            complement(198..419)
                     /locus_tag="ACHK49_005068"
     CDS             complement(198..419)
                     /locus_tag="ACHK49_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265519.1"
                     /translation="MDIQCTLANTFPNEHPEAFILVTSAEKFIVDGNCIQLIGIGNEQ
                     GEFDFYRQIYPVTGGEHKVEALMWTHLPA"
     gene            complement(503..883)
                     /locus_tag="ACHK49_005069"
     CDS             complement(503..883)
                     /locus_tag="ACHK49_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265520.1"
                     /translation="MNEIRSNPVKFLEEHLILNTQRYAHHDETSLITVNITREDGLLR
                     LKETEYDMAGSDYILFTAMRDTDSPERFDAPAVVSLSNQCISLRRNDISQTIQQSSPL
                     WLTNQQSGCSVLIVRHGLSESGEQ"
     gene            complement(1084..1659)
                     /locus_tag="ACHK49_005070"
     CDS             complement(1084..1659)
                     /locus_tag="ACHK49_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO9265521.1"
                     /translation="MPLTSDIGSRSFNLGLEVFRARIAANGRGDITVGGETVSIVYDA
                     TNGRFSSSGGNDGLLSELLILGFNNGPRALSERMLSMLSDSGEAQSQESIQDKISQCK
                     FPVSSGNFQCPPESIQCPITLERPEEGVFVKNSDSSAVCTLFDVDALSRVVNDGSVHP
                     LTRAPITPSMIVKPEECKYDPARGSFIIKDS"
BASE COUNT          512 a          378 c          291 g          484 t
ORIGIN      
        1 tacaaaaaaa atacggcaca acagaaaata gtagttgggt atcggcttct gttggttctc
       61 ttctggacaa aggagccaaa gtatatccag atatatcatg ctctctgaga ctgggagaac
      121 cattcattat tacacttcca gaaacagtca ggctggatgt taatatccac ccactcaaaa
      181 aatgatcctg aacgttttta tgcaggcaaa tgcgtccaca tcaaggcttc aaccttgtgc
      241 tctccccctg tcacaggata aatttgcctg taaaaatcaa actctccctg ctcattacca
      301 ataccaatca gttgaatgca gttgccatct acaataaatt tttccgctga cgtaaccaaa
      361 ataaaggctt ccggatgttc attaggaaag gtgttagcaa gagtacactg aatatccatc
      421 tccaacaaaa tctcctgatt attagcgtaa actcatcgac gaaatcgtta ctatcccctg
      481 ggcacatatg taccattgaa tactactgtt ctccagattc tgataatcca tggcgaacaa
      541 taagaacact acagccgctt tgctgattag ttagccataa cggtgaactt tgctggatag
      601 tttgagaaat atcatttcgc cggaggctta tacactgatt actcaagctt acaactgcag
      661 gtgcatcaaa gcgctccgga ctatcagtat cacgcatagc tgtaaacaga atataatcac
      721 taccagccat atcatattcc gtttctttta gtcttaacaa gccatcctct cttgtaatat
      781 taactgtaat taaagatgtc tcatcatgat gggcatagcg ctgtgtatta agaattaaat
      841 gctcttccag aaattttaca gggttactac gtatttcatt cattatacac cgaagctctg
      901 aacgatgcgg caccagaaag tgcccggtat tttgtgaaat aatactactt accttgattg
      961 ttttctctcc agctcatgaa aaataactcg cgtattatat tgttttacca accacaggcc
     1021 ccgaatgcct gtggtgtttt tatataaatt atcggaagtt aatattgttt catttgaaac
     1081 atcttaacta tcttttataa taaagcttcc ccttgcaggg tcatatttac actcttctgg
     1141 tttaacaatc attgatgggg ttattggagc tcgtgtcaga gggtgaactg aaccgtcatt
     1201 aaccacgcga gaaagtgcat caacatcaaa taaagtgcat actgctgaac tatctgaatt
     1261 tttgacaaac actccttctt cgggtctctc tagtgtaatt ggacactgaa tagactctgg
     1321 cgggcactgg aaatttcctg aactaacagg aaacttacat tgagatattt tgtcctgaat
     1381 actctcttgc gattgtgctt cacctgagtc cgaaagcata cttagcattc tctcactaag
     1441 ggctcgagga ccattattaa atcccaaaat caataactca gaaagcaatc cgtcattacc
     1501 gccactggat gaaaagcgcc cattagtagc atcatacaca atactgacag tttcaccacc
     1561 tactgtaata tctccgcgcc cattggctgc aattcgggca cgaaaaacct ccagcccaag
     1621 attaaatgaa cgtgatccaa tatctgaggt taatggcata acaag
//
LOCUS       DAZGLJ010000416         1656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000416 DAZGLJ010000000
VERSION     DAZGLJ010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.426"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..621
                     /locus_tag="ACHK49_005071"
     CDS             <1..621
                     /locus_tag="ACHK49_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO9265522.1"
                     /translation="KDSRINKNVVVLAGNHEINFNGNYTARLANHKLSAGDTYNLIKT
                     LDVCHYDSERKVLTSHHGIIRDEENACYCLGALQVPFKQMKNPTDPEELANIFNKKHK
                     EHMNDPLLHLIRSNIMKPTAVYADYINNTTDFRPKPERIFICGETLKCEDPSIYIRQK
                     YGHHGVGVDQNQQFDNGVMGLNSTKKVLDKNNKIIYSSGLSCFQSH"
     gene            883..>1656
                     /locus_tag="ACHK49_005072"
     CDS             883..>1656
                     /locus_tag="ACHK49_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO9265523.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     DNNYQFIVTINSINYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNV"
BASE COUNT          569 a          274 c          310 g          503 t
ORIGIN      
        1 aaagatagcc gtattaataa aaatgtcgtc gttctggctg gaaatcatga aatcaatttt
       61 aatggtaact atactgcacg actagcaaat cataaactct ctgccggtga tacgtataac
      121 ttaattaaaa cgctcgatgt ttgtcattat gactcagaaa ggaaagtttt aaccagtcat
      181 cacggaataa taagagatga agaaaatgca tgttattgcc tcggtgcttt gcaggttcca
      241 tttaaacaga tgaaaaatcc tactgatcct gaggaattag caaatatatt taataaaaaa
      301 cataaagaac atatgaatga tcctctatta catctaatcc gaagcaatat tatgaaaccc
      361 acagctgtat atgctgatta tattaataac actactgatt tcagaccaaa acctgaacgt
      421 atcttcatat gtggagagac actcaaatgt gaagatccca gcatatacat tagacaaaaa
      481 tatggccatc atggggtcgg ggtagatcag aatcaacaat ttgataatgg tgttatgggg
      541 ctaaattcca cgaagaaagt acttgataaa aataacaaaa taatttattc ctcagggctt
      601 agttgttttc aatctcatta aaactccatg aatacggtgg tatgcctttt taatcctgcc
      661 agtatttcat aggttttctt aatcgattgt agaggtatgt actgatgacg cctctacaat
      721 ctttagggac tttctctgct atgagataag aaatttcata ttatgcttta aggctgaatg
      781 atttgtgcaa aatataatta tttttccatc gaacatgagg tgtatgtcat aagattaaat
      841 gttaactatg cattcctcgc ggtgaattat atgaggtaaa agatgaaaat tggaactgtg
      901 gcaggcacta acgacagtac aacgacaatc gcaacaaatg atatggtgca agagcatgtt
      961 actaacttca ctaaagaact gtttgggtat attgcaaatg gtataggtga tgatatatca
     1021 agtattgcca ggacaatgct cggtgaagtt gtggaaaaaa ttgatgattg gcaaatagaa
     1081 cgtttccaac aatctattcg agatgacaaa atttcattta ccatacaaac gaatcattca
     1141 gaaaaatata gtatgctatc gggcatgcgc gctcatattc tacgtaggga taataattat
     1201 cagtttattg tgacaattaa tagtataaat tacggctgct atcttgataa taccgatatt
     1261 aattggtgca gcattgttta tttacttaat aatatgacag taaatgatga tgctaatgat
     1321 gttgcagtta ctgagtcgta caaacccatc tggaactgga aaatatcgca atacaatgta
     1381 tttgatatta aattcgaaac aatgataaaa ccccagttcg ctgacagaac ctatttctcc
     1441 aattgttcac ctgttgatcc cacaagtacc agaccaacct attttggcga taccgatggc
     1501 tcagtcggtg cagttttgta cgcgcttttt gcaacgggac atttgggaat catggctgaa
     1561 ggagaaaatt tccttagcca gttgcttaat atagaagatg aagttctcaa cgtattgcta
     1621 agggagaact ttaatgagca acttgacact aatgtt
//
LOCUS       DAZGLJ010000417         1648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000417 DAZGLJ010000000
VERSION     DAZGLJ010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.427"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(31..474)
                     /locus_tag="ACHK49_005073"
     CDS             complement(31..474)
                     /locus_tag="ACHK49_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO9265524.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            631..1560
                     /gene="metA"
                     /locus_tag="ACHK49_005074"
     CDS             631..1560
                     /gene="metA"
                     /locus_tag="ACHK49_005074"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO9265525.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          398 a          464 c          366 g          420 t
ORIGIN      
        1 tccagcctgg gcaggcacgg caggggcggc ttattccccc acatacgcca gattcagcaa
       61 cggatacggt ttccccaaat cgtccacctc agagcgtccc gtaaccttaa aacccacctt
      121 cttatagaac ccaaccgcct gctcattttg ctcattaacg ttggttgtca gttccggtgc
      181 catcgagagc gcatgctcca ccagcatccg acccacgccg cagccgcgca catcaggatc
      241 gataaacagc gcatccatat gctgcccact taacaacata aacccaaccg gctgatcccg
      301 ttcattaacc gcgacccaca acggtgcttc cggcagaaag gaacgaacca gctcttccag
      361 ctcggcccga tactctgctg ataaaaaatc gtgggtggca tcgacagatc gacaccaaat
      421 cgcaacgagt tcctcccctt cctcatgctg tgagcggcga atactaataa ccattttctc
      481 tccttttcgt cattcttata ttctaacgta gccttttcct tgaaactttc tcaccttcaa
      541 catgcaggct cgacattggc aaattttctg gttatcttca gctatctgga tgtctaaacg
      601 tataagcgta tgtagtgagg taatcaggtt atgcctattc gtgtgccgga cgagctaccc
      661 gccgtcaatt tcttgcgtga agaaaacgtc tttgtgatga caacttctcg tgcgtctggt
      721 caggaaattc gtccacttaa ggttctaatc cttaacctga tgccgaagaa gattgaaact
      781 gaaaatcagt ttctgcgcct gctttcaaac tcacctttgc aggtcgatat tcagctgttg
      841 cgcatcgatt cccgtgaatc gcgcaacacg cccgcagagc atctgaacaa cttctactgt
      901 aactttgaag atattcagga acagaacttt gacggtctga ttgtaaccgg tgcgccgctg
      961 ggcctggtgg agtttaatga tgtcgcttac tggccgcaga tcaaacaggt gctggagtgg
     1021 tcgaaagatc acgtcacctc gacgctgttt gtctgctggg cggtacaggc cgcgctcaat
     1081 atcctctacg gcattcctaa gcaaactcgc accgacaaac tctctggcgt ttacgagcat
     1141 catattctcc atcctcatgc gcttctgacg cgtggctttg atgattcatt cctggcacca
     1201 cattcgcgct atgctgactt tccggcagcg ttgattcgtg attacaccga tctggaaatt
     1261 ctggcagaga cggaagaagg ggatgcatat ctgtttgcca gcaaagataa gcgcattgcc
     1321 tttgtgacgg gccatcccga atatgatgcg caaacgctgg cgcaggaata tttccgcgat
     1381 gtggaagccg gactaggccc ggaagtaccg tataactatt tcccgcacaa tgatccgcaa
     1441 aatacaccgc gagcgagctg gcgtagtcac ggtaatttgc tgtttaccaa ctggctcaac
     1501 tattacgtct atcagatcac gccatacgat ctacggcaca tgaatccaac gctggattaa
     1561 tcttctgtga tagtcgatcg ttaagcgatt cagcacctta cctcaggcac cttcgggtgc
     1621 cttttttatt tccgaaacgc acctcagg
//
LOCUS       DAZGLJ010000418         1647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000418 DAZGLJ010000000
VERSION     DAZGLJ010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.428"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            103..1641
                     /locus_tag="ACHK49_005075"
     CDS             103..1641
                     /locus_tag="ACHK49_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc22 family transposase"
                     /protein_id="HFO9265526.1"
                     /translation="MSSSLPDDINALKRLLAEQEALNRALLEKLNEREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLKALQKESDTLTGRVDDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAASCCPECGGSLSYLGEDAAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLISKYAEHTPLYRQSEMYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALQDYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNA
                     GSTLAPAVLFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKN
                     YLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVA
                     LPTE"
BASE COUNT          372 a          472 c          499 g          304 t
ORIGIN      
        1 ggtatcgact ggcggcagcc taaaagactg cttacgtccc tgactatgtt gtaagcctct
       61 ttatcctggt cgacgctgaa tgagcctggt aatatacccg gtatgagcag ctcacttcct
      121 gacgatatca atgcactgaa acgtctcctt gccgaacagg aggcgctgaa ccgtgccctg
      181 ctggaaaagc tgaacgagcg tgaacgcgaa atagaccatc tgcaggcaca gctggataag
      241 ctgcgccgga tgaacttcgg cagccgctcc gaaaaagtct cccgtcgtat cgcacagatg
      301 gaagctgacc tgaaggcact tcagaaagaa agtgataccc ttaccggtcg ggttgacgac
      361 ccggccgtgc agcgcccgct gcgtcaaacc cgcacccgca aaccgttccc cgaatcactc
      421 ccccgcgatg aaaaacggct gctgccggca gcatcatgct gcccggaatg tggaggctca
      481 ctgagctatc tgggtgagga tgccgccgaa cagctggagt tgatgcgcag cgccttccgg
      541 gttatccgga ctgtacgtga aaagcatgcc tgtactcagt gcgatgccat cgtgcaggcc
      601 cccgcgcctt cacggcccat cgagcggggt atcgcaggac cggggctgct ggcccgcgtg
      661 ctgatctcaa agtatgcaga gcacaccccg ctgtaccgcc agtctgaaat gtacggccgc
      721 cagggcgtgg agctgagtcg ttcactgctg tcgggctggg tggatgcatg ctgccggcta
      781 ctgtcaccgc tggaagaagc gcttcaggac tatgtgctga ctgacggtaa gctccatgct
      841 gatgacacgc ctgtcccggt gctgttgcca ggcaataaga aaacgaagac cgggcggtta
      901 tggacctacg ttcgtgacga ccgtaacgcc gggtcaacgc tggcgccggc ggtgttgttc
      961 gcttacagcc cggacagaaa aggcatccat ccgcagaccc atcttgcggg gttcagtggt
     1021 gtactgcagg cggatgcata cgccgggttc aacgagctgt accgggatgg ccggataacg
     1081 gaagccgcct gttgggctca cgcccgccgt aaaatccacg atgtgcacgt tcgcaccccg
     1141 tcagccctga cggaggaagc gctgaaacgg atcggcgaac tgtacgccat cgaggcagag
     1201 ataaggggaa tgacggcgga gcagcgcctt gccgaacgtc agttgaaaac gaaaccgctg
     1261 ctgaaatccc tggaaagctg gctgcgtgaa aagatgaaaa ccctgtcgcg acactcagaa
     1321 ctggcgaaag cgttcgcata cgccctgaac cagtggccgg cgctgacgta ctatgcagat
     1381 gatggctggg ctgaggcgga caataacatc gctgaaaatg cgttgcggat ggtcagtctg
     1441 ggccgcaaaa actacctgtt cttcggttcg gatcatggag gagagcgggg agcgctgctg
     1501 tacagcctga tcgggacgtg caaactgaac ggagtggagc cagaaagcta cctccgctat
     1561 gtccttgacg tcatagccga ctggccgata aaccgggtcg gcgaactgct cccctggcgc
     1621 gtagcactgc cgactgaata acacatc
//
LOCUS       DAZGLJ010000419         1630 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000419 DAZGLJ010000000
VERSION     DAZGLJ010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.429"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..236
                     /locus_tag="ACHK49_005076"
     CDS             <1..236
                     /locus_tag="ACHK49_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265527.1"
                     /translation="NKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKTTPPVGNV
                     QGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            238..519
                     /locus_tag="ACHK49_005077"
     CDS             238..519
                     /locus_tag="ACHK49_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFO9265528.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            516..788
                     /locus_tag="ACHK49_005078"
     CDS             516..788
                     /locus_tag="ACHK49_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265529.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFTELVAMKASALMKDMDMSSGVIKGNEFNQQG"
     gene            793..1086
                     /locus_tag="ACHK49_005079"
     CDS             793..1086
                     /locus_tag="ACHK49_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265530.1"
                     /translation="MSKVRVIFEFDHVMHEVKPAGNDSEEITEGVTATVKIERDTENR
                     PAGPCDVYAQILKYHSPTIIQFLTDELQGSMQAMGVSSSVERRSVQNGPDTLQ"
     gene            1098..1628
                     /locus_tag="ACHK49_005080"
     CDS             1098..1628
                     /locus_tag="ACHK49_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO9265531.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGTPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
BASE COUNT          474 a          352 c          472 g          332 t
ORIGIN      
        1 gtaacaaaat gaatttattc gactccctga acagcgcgcg ccgtctgacc gaacttgccg
       61 gtgcggtact ggaacgcagt aagcgctatc cgcaacgttt tgcactgaaa accacgccgc
      121 cggtaggcaa cgtgcaggga accggtgaaa ttgaaatcac catacagacc aacggcctgc
      181 gtcgccgtgt gaaggccacc cgcatcagcg gctgcacggt ttactgggag gtatgaggtg
      241 aaaaaaaatc tcattgcatg ggcgtggtcg agtggtctta ttgagtttgg ttacgtcctg
      301 ccggaaggtg cattaccgat agttgccgga aagcctgcca cggtacggca tgtgattgag
      361 gttatggcgc gtcatggacg tgatgaacag gagcagttac tggttccggg gataccggaa
      421 gcggtgacgg aggaagaagc ctttaatgcc atgattcggt tctgccgtga ggtcagacgc
      481 cgggtcagtt atccaaacag aacgaggacc agaggatgag taaagttgta cgcattattt
      541 tcgaatacaa ggagcacgtt atccataaaa acgcggatgg aacagtgcgc atgggggtaa
      601 gtctggacat acgttcaacc gggataaagc agaaaggtga tggacctgcc atgatttttg
      661 gggtggttat gctcgcggaa agcagagact ttactgaact tgtggcaatg aaagccagtg
      721 cgctcatgaa agatatggac atgagttccg gggttattaa aggtaatgaa tttaatcagc
      781 aggggtaatt ccatgagcaa agtacgcgtt atttttgaat ttgaccacgt aatgcatgaa
      841 gtaaagccag ccggtaatga cagtgaggaa atcacggagg gggttactgc cacggtaaaa
      901 atcgaacgtg atacagagaa cagaccggca ggaccctgtg atgtttatgc acaaattctt
      961 aaatatcaca gccccacaat cattcagttt ctgacagatg aactacaggg ttccatgcag
     1021 gccatggggg taagcagctc cgttgaacgc cgttctgttc agaacggacc agatacattg
     1081 caataaggaa aaacaaaatg gcaaaacgcg ttacaaaatt aaaggccgca gcagaggcgg
     1141 caccgcagac ccgcgaagaa gtcagccgcg atatccgcac cctgggcgat attcagcgag
     1201 aggcgctgcg cctggaaaca gcgatgaatg acgaggtggc agaaatcacc gcccgttata
     1261 cgccgcagat tgaaaacctt aaaaaagaaa tcaaagtgct ttttaagggg attcaggact
     1321 ggtgcaaaac caaccgcgat gagctgacga acggcggcaa aacaaaaact gccaatctga
     1381 ccaccggaac ggtgtcatgg cggctgggaa cgccatcatg cagcgtcagc cgtgatgtgg
     1441 aaggtgtgat tgaaatgctg cgccgtatgg gtcttgagcg attcatccgc acgaaagagg
     1501 aagtgaacaa ggaagccgtc ctggcggagc cggatgcggt gaaagggatt gccggtatta
     1561 aggtaaataa aggcgctgaa agtttttatg tcgagccttt tgaacaggac gccggactga
     1621 acaaataaca
//
LOCUS       DAZGLJ010000420         1622 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000420 DAZGLJ010000000
VERSION     DAZGLJ010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.430"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..219
                     /locus_tag="ACHK49_005081"
     CDS             <1..219
                     /locus_tag="ACHK49_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9265532.1"
                     /translation="ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDR
                     CSKCMRGCEMRGMVANFGGFLSINKLSQ"
     gene            224..967
                     /locus_tag="ACHK49_005082"
     CDS             224..967
                     /locus_tag="ACHK49_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO9265533.1"
                     /translation="MTETESAILAHARRCAPAESCGFVISTPEGDRYQPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDACHLAGITLPDFHREDEWWRNGQNLYLDNMEAT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHVSAFTGIYNDLAAASACM"
     gene            964..1545
                     /locus_tag="ACHK49_005083"
     CDS             964..1545
                     /locus_tag="ACHK49_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO9265534.1"
                     /translation="MNTAAEAIRALSMQMPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKVPEYKSTDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRQA"
BASE COUNT          339 a          415 c          523 g          345 t
ORIGIN      
        1 gcgctgtttc ctggtcgcat catgctggcg aacacctgta tgtgggatta ccggggagat
       61 gaatgcgggt ataacggtcc tgcggtggcg gatgagttcg acaaccccac cacggatatc
      121 cgtaaggaca gatgcagcaa gtgcatgcgc gggtgtgaga tgcgcggcat ggtggctaat
      181 tttggcggtt tcctttccat taataaactt tcgcagtaaa tccatgacag agacagaatc
      241 agcgattctg gcgcatgccc ggcggtgtgc gcctgcggag tcttgcggct tcgtgataag
      301 cacgccggag ggtgaccggt accagccgtg cgtgaatatc tctgcagagc ctgaggcata
      361 ttttcgtatt gccccggaag actggctgcg ggcagagatg cagggggaga ttgtggcact
      421 ggtccacagc catcccggtg gcctgccctg gctgagcgag gccgaccggc ggctgcagat
      481 aaaaagtgcc ctgccctggt ggctggtctg ccggggggac attcataaat tccgctgtgt
      541 gccgcacctg accgggcgtc gttttgaaca cggggtgacg gactgttaca cgctgttcag
      601 ggatgcctgc catctggcgg ggataacgct gccggatttt caccgcgagg atgagtggtg
      661 gcgcaatggt cagaacctgt atctggacaa tatggaggcc actggttttt accgggtgcc
      721 cctgtcctcg gcacaggcag gcgatatcct gctgtgctgc tttggcgcat cggtacccaa
      781 ccatgctgcc atttactgcg gcaacggtga actgcttcac catctgcctg aacaactgag
      841 taaacgggag aggtattccg aaaaatggca acgacgaacg cattctgtct ggcgtcaccg
      901 ccactggcac gtatctgcct tcacggggat ttacaacgat ttggccgccg cctcagcctg
      961 tatgtgaaca cggcagcgga agccatccgt gccctgtcga tgcagatgcc gggattccgc
     1021 cgtcagatga acgaaggctg gtaccagata cgtattcgcg gtgaggacac ggcaccggag
     1081 gcggtgtacg cccgtcttca cgaacagctg ggtgagggaa cggtcatcca cattgtgccg
     1141 cgactggccg gggccggaaa gggtggactg cagattgtgc tgggggcggc agccatcgtg
     1201 ggctctttct tcactgccgg ggcatcaatg gcgttatggg gttcagccct ggcagccggt
     1261 ggtttttctg ccaccacgat gctgttttca ctgggggcca gcatgatact gggtggtgtg
     1321 gctcagatgc tggccccgaa ggctaaagta ccggagtaca aaagcacgga taacggcaga
     1381 cagaacacgt acttttcctc gctggataac atgattgcac aggggaaccc gatgccggtg
     1441 ccttacggtg aaatgctggt tggctcacgg cgaatctccc aggacatcag tacccgtgat
     1501 gaaggcggtg acgggaaggt ggtggttatc gggcggcagg cataaaagcg aaaaaatccc
     1561 gcagtgaccg aaggctgcgg gaacagaaaa tgaagattaa ccacagggag ttttgttttt
     1621 at
//
LOCUS       DAZGLJ010000421         1613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000421 DAZGLJ010000000
VERSION     DAZGLJ010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.431"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1169
                     /gene="rhsB"
                     /locus_tag="ACHK49_005084"
     CDS             <1..1169
                     /gene="rhsB"
                     /locus_tag="ACHK49_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO9265535.1"
                     /translation="VTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELA
                     KTQRRSLADTLQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLT
                     VEQMQNQMDPVYTPARKIHLYHCDHRGLPLALISKEGATEWCAEYDEWGNLLNEENPH
                     QLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYGYQLNPISD
                     IDPLGLSMWEDAKSGACTNGLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKE
                     FAGLICKDNSGRYFSTAPNRGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEI
                     FSGKDEKIVKSKDNNIKSFYLGTPSGNFKAIDNHGKEITNRKGLPNVCRVHGNM"
     gene            1141..1524
                     /gene="yhhH"
                     /locus_tag="ACHK49_005085"
     CDS             1141..1524
                     /gene="yhhH"
                     /locus_tag="ACHK49_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HFO9265536.1"
                     /translation="MSAEFMVICKKLLFRNCVIVSLFVFTYNTWAQCNNNIKIMRKYE
                     SEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSD
                     QIAGGVFIIEIGKNDGRILNFGHGK"
BASE COUNT          505 a          319 c          419 g          370 t
ORIGIN      
        1 aagtgacctg gtacggctgg gacggcgacc ggctgaccac gatacagaac gacagaaccc
       61 gcatccagac gatttatcag ccggggagct tcacgccact catcagagtt gaaaccgcca
      121 ccggtgagct ggcgaaaacg cagcgccgca gcctggcgga tacccttcag cagtccggcg
      181 gcgaagacgg tggcagtgtg gtgttcccgc cggtgctggt gcagatgctc gaccggctgg
      241 aaagtgaaat cctggctgac cgggtgagtg aggaaagccg ccgctggctg gcatcgtgcg
      301 gcctgactgt ggagcagatg caaaaccaga tggacccggt gtacacgccg gcgcgaaaaa
      361 tccacctgta ccactgcgac catcgcggcc tgccgctggc ccttatcagt aaggaagggg
      421 caacagaatg gtgcgcagaa tacgatgagt ggggcaacct gctgaatgaa gagaacccgc
      481 atcagctgca gcagcttatc cgcctgccgg ggcagcagta tgatgaggag tccggcctgt
      541 attacaaccg ccaccgctat tatgacccgc tgcaggggcg atatatcact caggatccga
      601 ttggactgaa ggggggatgg aacctgtatg gatatcaatt gaatccgata tcagacatcg
      661 accccctggg tttatctatg tgggaggatg caaaatcggg ggcatgtact aatggtcttt
      721 gcggcacact atccgctatg ataggtccag ataaatttga ttctatagat agcaccgcat
      781 atgacgcctt aaataaaata aatagccaat ctatttgcga agataaagag ttcgctggtt
      841 taatatgtaa ggataatagt ggcagatatt tctcaacagc acctaaccga ggagaaagaa
      901 aaggatcata tccattcaat agcccttgcc ctaatggtac tgagaaagta tcagcttatc
      961 atactcatgg tgcagatagt catggagaat attgggacga aatattttca ggtaaagatg
     1021 agaaaatagt taaaagtaaa gataacaata tcaagtcatt ttatttaggt acgcccagtg
     1081 gtaattttaa agcaatagat aaccacggga aggaaataac aaacagaaaa ggattaccta
     1141 atgtctgcag agttcatggt aatatgtaaa aaactattgt ttaggaactg tgtcattgta
     1201 tctttgtttg tttttacata caacacatgg gcgcagtgta ataataatat taaaataatg
     1261 cgcaagtatg aaagtgaagg taaatatacc gttagaaatt tggttaaaaa taaagctata
     1321 gcattggaat tagctgagat atatgttaag aatcgttatg gacaggatgc cgcagaagaa
     1381 gaaaaaccat acgaaatcac tgagttaaca acaagttggg ttgttgaagg taccattcac
     1441 tcagaccaaa ttgctggtgg ggtttttatt atagaaatag gcaaaaatga tgggagaatt
     1501 ctgaattttg gccacggaaa ataagtatgt aaggataatc aaacatcgtg tgcgctgatg
     1561 gcagagtagg tggaggactc cagacagtca aacgatagaa aaagatagcc ttt
//
LOCUS       DAZGLJ010000422         1603 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000422 DAZGLJ010000000
VERSION     DAZGLJ010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.432"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            125..304
                     /gene="yciG"
                     /locus_tag="ACHK49_005086"
     CDS             125..304
                     /gene="yciG"
                     /locus_tag="ACHK49_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO9265537.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            390..890
                     /gene="yciF"
                     /locus_tag="ACHK49_005087"
     CDS             390..890
                     /gene="yciF"
                     /locus_tag="ACHK49_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO9265538.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            936..1442
                     /gene="yciE"
                     /locus_tag="ACHK49_005088"
     CDS             936..1442
                     /gene="yciE"
                     /locus_tag="ACHK49_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO9265539.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1502..>1603)
                     /locus_tag="ACHK49_005089"
     CDS             complement(1502..>1603)
                     /locus_tag="ACHK49_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HFO9265540.1"
                     /translation="IDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT          523 a          324 c          352 g          404 t
ORIGIN      
        1 atattttccc tctatcttct cgttgcgctt aatttgacta attctcatta gcgactaatt
       61 ttaatgagtg tcgacacaca acactcatat taatgaaaca atgcaacgca acgggagaaa
      121 taacatggcc gaacatcgtg gtggttcagg aaatttcgcc gaagaccgtg agaaggcatc
      181 cgacgcaggc cgtaaaggcg gtcagcatag cggcggtaat tttaaaaatg atccgcaacg
      241 cgcatctgaa gcgggtaaaa aaggcggtca acaaagcggt ggtaataaat caggcaaatc
      301 ctgattcacc gttaattatt aatatcagct gataaagata attgcatctg cgggccgtat
      361 ggctcgcagt gatttcactg gagaaaaata tgaatatgaa gaccattgaa gatgtattta
      421 ttcacctgct ttcagatacc tacagcgcag aaaaacaatt aacccgggca ctggcaaaac
      481 tcgcaagagc aacatcaaat gaaaaattaa gtcaggcttt tcatgcgcac ctcgaggaaa
      541 ctcatggaca gattgaacgt attgatcaag ttgtggagtc cgaatcgaac ctgaaaatta
      601 agcgcatgaa atgtgtggca atggaaggtc ttattgaaga agctaatgag gtcatcgaaa
      661 gtaccgagaa aaacgaagtg cgtgatgccg cactgattgc cgcagcgcag aaagtcgagc
      721 attatgagat tgccagttac gggacattag cgacgctggc tgaacaatta ggttaccgta
      781 aagcagcgaa gcttctgaaa gaaaccctgg aagaagaaaa ggccaccgac atcaaactga
      841 ctgatctggc ccttaataac gtaaataaga aagccgaaaa taaagcctga aatatgaatt
      901 ttaactttta gtcattttat aaagaggaca ttttcatgaa tcgtattgaa cattatcatg
      961 actggttacg tgacgcccac gcaatggaaa agcaagccga atctatgctt gaatccatgg
     1021 ccagccgtat agataattat cctgaactac gcgctcgtat tgaacaacat cttagtgaaa
     1081 ccaaaaacca gattgttcaa ctggaaacta ttcttgatcg taatgacatt tcacgttcag
     1141 tcattaaaga ttccatgagt aaaatggctg cgcttgggca gtcaatcggt ggtatattcc
     1201 cttctgatga aatagttaaa ggctctatta gcggatatgt cttcgagcaa tttgaaatcg
     1261 cctgttacac ctcactatta gcagcagcaa aaaatgccgg tgatacagtc tcaattccga
     1321 tcatcgaagc gattttaaat gaggaaaagc aaatggccga ctggctgatt cagcatattc
     1381 cgcaaacaac tgagaaattt ttaattcgtt ctgaaactga tggcgtagaa gcgaagaaat
     1441 aatacccttt ataccatgtc cttattgacc ccgtatatta cggggtcgtt tttgtgcgga
     1501 attaaaaacg atatcctgct gagaacataa acacccacgg atcgaggcgt acgctatcgt
     1561 gttgctgtgc accgcctagc ttataattgg cggtggtatc gat
//
LOCUS       DAZGLJ010000423         1601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000423 DAZGLJ010000000
VERSION     DAZGLJ010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.433"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..65
                     /locus_tag="ACHK49_005090"
     CDS             <1..65
                     /locus_tag="ACHK49_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO9265541.1"
                     /translation="CCAELMSDPNSSMYEEEDDE"
     gene            58..780
                     /locus_tag="ACHK49_005091"
     CDS             58..780
                     /locus_tag="ACHK49_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO9265542.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEVSERINNLGFNVQYEHYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEETAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            780..1070
                     /locus_tag="ACHK49_005092"
     CDS             780..1070
                     /locus_tag="ACHK49_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO9265543.1"
                     /translation="MANLRKESRGRECQVRIYGICNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            1067..1429
                     /locus_tag="ACHK49_005093"
     CDS             1067..1429
                     /locus_tag="ACHK49_005093"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9265544.1"
                     /translation="MNEYQFVLPYPPSVNTYWRRRGSQYYISDKGQKYRKDVQQIIHQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENA"
     gene            1426..>1601
                     /locus_tag="ACHK49_005094"
     CDS             1426..>1601
                     /locus_tag="ACHK49_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFO9265545.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGK"
BASE COUNT          481 a          371 c          411 g          338 t
ORIGIN      
        1 actgctgcgc agaactgatg agcgatccga atagctcaat gtacgaggaa gaagacgatg
       61 aatgagttaa taaatggcaa tgccatcaaa atgacaagca ttgaaatagc tgagttggtg
      121 ggtaagcgtc atgacaatgt gaaacgtacc atcgaaacgc tggctaaaaa tggtgttatc
      181 cggcttcctc aaattgaggt ttccgaaaga atcaataact tagggttcaa tgttcagtac
      241 gagcattacg tcttcgaggg cgaacaaggt aagcgagaca gtattgtcgt tgttgcccag
      301 ttgtcgccag agttcaccgc tcgtcttgtt gaccgttggc gagagcttga agaaactgcg
      361 gttaatatcc ccaaaacgtt accggaagcg ttgcgccttg ctgctgatct tgctgagcag
      421 aaaatgcaac tggaaaacca gctcgcaatt gccgcaccta aagttgagtt tgccgatcgc
      481 gttggcgagg ccagcggaat tttgattgga aactttgcaa aggttgttgg tattggtcca
      541 aacaaactgt ttgcgtggat gcgcgatcac aaaatcctta ttgcttcagg ttcccggcgc
      601 aatgtgccaa tgcaggaata tatggatcgc ggctatttca cagtgaaaga aacagcggtc
      661 aacacaaatc acggaataca gatatcgttc accacaaaaa tcaccgggcg tggtcaacag
      721 tggctgacca gaaagctgct cgataacgga atgctgaaag taacagggga ggctgcttaa
      781 tggctaacct acgcaaagaa tctcgcggca gagaatgcca ggtacgtatt tacggcatat
      841 gcaatggcaa tcctgaaact acagttctgg cacattaccg gatggctgga atttgcggaa
      901 cgggaatgaa gcctgacgac ctgatcggcg catgggcttg tagcgcgtgt cacgatgaaa
      961 tcgaccgacg cacccataac ctcgacaaca aagacgccag actttaccac ctcgaaggcg
     1021 tgatcaggac gcaggcgata ctgctgaagg aggggaagat taagtcatga acgaatatca
     1081 gtttgtgctt ccatacccgc cgtcggtgaa tacctactgg cgaagacggg gaagccaata
     1141 ctacatcagc gataaaggcc agaaataccg aaaagacgtt cagcaaatca tccaccaact
     1201 caagttagat attttcacca aatcacgact ccgcatcaaa gtaatcgcag acgttccaga
     1261 ctcccgccgc cgcgacctag ataacatcct gaagggttta ctcgactccc ttatccatgc
     1321 cggatttgcg gaagacgacg agcaattcga tgacattcgc gtaattcgtg gtgtgaaagt
     1381 accaggcgga cggcttggaa taaaaatcac cgaactggag aacgcatgaa cgccacaatt
     1441 caaacgatac cagagcttct tatccagaca cgaggcaatc agaccgaagt ggcgaggatg
     1501 ctttcctgcg caagaggaac agtgctcaag tacaaccgag acagcaaagg cgagcgtcat
     1561 gtaatagtta acggcgtcct gatggtcaaa cagggcaaga g
//
LOCUS       DAZGLJ010000424         1596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000424 DAZGLJ010000000
VERSION     DAZGLJ010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.434"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1596
                     /locus_tag="ACHK49_005095"
     CDS             <1..>1596
                     /locus_tag="ACHK49_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265546.1"
                     /translation="PNSTSNKGYVCIDMQCSSTSEPTASTSSNRSIKLAASTNVYPIT
                     RNDSELTLNDFLDNSSSTSSLDYINELGSQLTLNDFLDNIKTNEVDRTCTDVVINIPQ
                     EIQTNTQENDLLLSDKNNSICIEIDERITKILTCKQKYQLDSIIHEIIPKENESAETV
                     LHLMRVLNDQYHQVYNQSGCFYKAYMAIHNKIEQILPYAFRAGGGISIHLLIQALFFN
                     GDYNKSPSQSQQPSLYTSPSPTINTEAFLSNVLSLDITQVRILGDLLSATLFHAPTIF
                     YQYPKLIDEVKYCISNKKITGSVIARFTLCLTSTLLTMSPLLMLNGAVKTGSIVRTIG
                     RGVSYVDIPLALAILGDSWYKAYKHGSSDNPNSAQRFISQEAAFKTTQRVLTQGLSLM
                     SSLSGAIMRSLEKGTPPQMMSLFIVNILNLLFHQNPYEGASASANALKRSTYSHNPDI
                     LNTQAIALCVDLQHTKNITMPLFKTKDRISYAFNGQRTSPEDQKQILKEVINSCTQGE
                     RAILNTSQSETCKHKIDEIYEKR"
BASE COUNT          591 a          315 c          250 g          440 t
ORIGIN      
        1 ttccaaattc aacttcaaat aaaggttatg tatgcattga catgcagtgt tcgtcaacct
       61 ctgaaccaac agcttctacc tccagcaacc ggagtattaa attagccgct tccacaaatg
      121 tatatccgat aacaagaaac gactccgagc tcactctgaa cgattttctt gataatagct
      181 cttctacgtc atcattggac tacattaatg aattgggttc tcaactgacg ttaaatgatt
      241 ttcttgacaa cataaagaca aatgaggtgg atagaacatg tacggatgtg gtaattaata
      301 tcccacaaga gatacaaaca aatacacagg aaaatgattt gttattatcc gataaaaata
      361 attcaatatg cattgaaatc gatgaaagaa ttacaaaaat cctaacatgc aagcaaaaat
      421 atcaactgga cagcatcatt catgaaatta taccaaaaga gaatgagagt gcagaaactg
      481 ttctccatct tatgagagtt ctgaacgatc aatatcatca ggtatataat cagtcaggat
      541 gtttttataa agcctatatg gccatacaca ataaaatcga acagatactt ccatatgcgt
      601 tcagagccgg aggcggaatc agcattcact tgctcataca ggcattattt tttaatggcg
      661 actataacaa atcaccttca cagtctcaac aaccatcttt atatacatca ccttctccaa
      721 caataaatac agaagcattc ttaagtaatg tattatcact agatataacc caggtacgca
      781 tacttggtga tttactatca gcaactttat ttcatgcacc aacaatattc tatcaatatc
      841 ctaaactaat agatgaagtt aagtattgta taagtaataa aaaaataaca ggttcggtta
      901 tagcacgatt tactctatgt ttaacaagta cattactaac catgtcacca ctgttaatgc
      961 ttaatggagc agttaaaaca ggtagcatag taagaactat aggtagggga gtgagttatg
     1021 ttgatatacc attggcctta gctatattag gtgactcgtg gtataaagct tataaacatg
     1081 gttcttctga taacccaaat tctgctcaga gatttatatc gcaagaagcg gcctttaaaa
     1141 ccacccagcg ggtattaaca caaggattaa gtctgatgtc ctctttatcg ggagcaatca
     1201 tgcgctctct tgagaaaggc acaccaccac aaatgatgtc tttattcatc gtaaacatac
     1261 taaatctatt atttcatcaa aatccatatg aaggggcatc agcaagtgct aatgctttga
     1321 aaagatcaac ttactcccat aatccggaca tactaaatac tcaggcaata gctctttgtg
     1381 ttgacctcca gcatacaaaa aatataacca tgccactttt caaaacaaaa gacaggatat
     1441 cttacgcatt caatggacaa agaacatccc cagaagacca aaaacaaata ctgaaagaag
     1501 ttataaactc ctgtacccaa ggagaaagag ccattttaaa tacatcacaa tcagaaacat
     1561 gcaaacataa aatcgatgag atttatgaaa aaagat
//
LOCUS       DAZGLJ010000425         1591 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000425 DAZGLJ010000000
VERSION     DAZGLJ010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.435"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..395)
                     /locus_tag="ACHK49_005096"
     CDS             complement(<1..395)
                     /locus_tag="ACHK49_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFO9265547.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMLS
                     FFGQYKTLIEDLIEHFRYGNGSNFHSQQL"
     gene            576..728
                     /locus_tag="ACHK49_005097"
                     /pseudo
     CDS             576..728
                     /locus_tag="ACHK49_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            1352..>1591
                     /locus_tag="ACHK49_005098"
     CDS             1352..>1591
                     /locus_tag="ACHK49_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265548.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLG"
BASE COUNT          499 a          233 c          310 g          549 t
ORIGIN      
        1 tttaattgtt ggctatgaaa attgctgcca tttccatatc taaaatgttc aatcagatct
       61 tcgataagtg ttttatattg tccaaaaaag gaaagcattt ttgctagttc tttcatttct
      121 gcaaataaaa tatcagtgca ttcacgttgt gatatttttg ttccggaact tgaggcacta
      181 aaagcaacat taatagaacc tgggccaggg aaatggtatt taataagtct gtaaggatca
      241 acttttgcag agatatcgct taggccgagt gagagaaagt cttgttctac catatcacca
      301 tattgcatat catcagcaga aaaatcatcg aataaactaa tagtttcgaa aagtgtacag
      361 ggaatattaa ttggcggaag taatatgtta ttcatttgca tttgccttgg ttatatttaa
      421 gctcaatatc aattcgagaa caaatgctag ggtaaaaaaa tatttaaaaa agaagtaaac
      481 ttcacatttt ttcattacat ctctgaagag aacctcgtta atgattggtc ttgctcattg
      541 ttaataacaa aatgttgaat ttaccgatta ggaaactttt tcctacgcgg attgcctcca
      601 gctatggcat gaactttgag tttatgccag aactgaagtg gggcttcggc taccctggcg
      661 cgattatctt tatgatcctc gcgggcctga caccgtatct gtactttaag cgtaagaact
      721 ggctgtaact atacgatgtc gctgtgagta agttagtgtg ctgacagcga cattttagcc
      781 gagggaatcg cagataacat aattgttgtg aataatatcc atgccacgtt gagttggacg
      841 cttaatgact cacatctcac cccttcaatt gctgcagaga taatagaaaa aagtgtcaag
      901 cccaatgaaa tcaatacaaa gtagattgcc ttacatccag tatctcttat tctttttgct
      961 ccaatattga tatttacata ggtattatta ataaccagaa taaaactcaa agccaggttg
     1021 atatcattca aagttattaa tattgaccat gatgttaatg tatagattat gaactgaaag
     1081 cgtgataaac gctcaagcct ggatgggtag aatatatttc tgatggatga tttgaacatt
     1141 tttacattgt tttatatata attgttttga atatatatta cctgaattat gtggtttgca
     1201 tatatatatg caatatagca gtctttgcat tgataactat acagtagtaa tttagagtgt
     1261 gtaagttttt attgatgggg tatattttaa ttaaatataa ttgaaatgta tttttattct
     1321 ataaacaata tcttgggtga atggattcta tatgggaaat ggtaactttt ggcagttgtt
     1381 cttgggtaaa gaaccttaca gcaaaaaatc tgattttgtt aaggcatggt tattgtggtg
     1441 ggctattacc atccttgttc tatttatttt aagcctgact acgacattaa atttaagaag
     1501 tataggtata gggttaagtt ttgggttttg tcaaatcctt ggtagatata cgatgtctct
     1561 aggatggagt aagtactggg gagttttggg g
//
LOCUS       DAZGLJ010000426         1588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000426 DAZGLJ010000000
VERSION     DAZGLJ010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.436"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            14..1336
                     /locus_tag="ACHK49_005099"
     CDS             14..1336
                     /locus_tag="ACHK49_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO9265549.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFDLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            1364..>1588
                     /locus_tag="ACHK49_005100"
     CDS             1364..>1588
                     /locus_tag="ACHK49_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFO9265550.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILI"
BASE COUNT          402 a          329 c          357 g          500 t
ORIGIN      
        1 caggtataaa aaaatgggat taatggcctc attcgaacgt ggaatggaac gttttcttgt
       61 tccagttgct atcaagttaa actcacaaaa acatgttgca gcggtgagag atggattcgt
      121 ttttacgttt ccaattatca tggcaagttc attaattata ttaattaact ttgccatatt
      181 atcgccagac ggttttattg ccggattact gcatctgaat agcattttcc ccaaccttga
      241 aaaagcacaa gctattttta ctccggtaat gaatggttct gtaaatatca tgtcaattat
      301 gattgctttc ctggtcgcca ggaatatggc gattagctat gagcaagatg atcttttatg
      361 cggattaacg gcaataggag cattttttat tgtttatacg ccatatcaga tgatagatgg
      421 gcaagcattc ctgacgacca aatatctcgg cgcgcagggg ttgtttgttg ctgttatcgt
      481 tgcattgatc accagtgaaa tattttgtcg cttagctcga aacccaaaaa tcaccataac
      541 gatgccggca gctgtaccgc ctgcggtagc gcgttcattt aaagttttat tgccaatatt
      601 ttttgtcatg gtgttctttt ccgcacttaa ttattgcctg acactgatat ccccggcagg
      661 actaaacgat cttatttaca cattaatcca gacgccgctc aaacatatgg gaacgaatat
      721 ctttgcggta attatcctgg gggctgtggg taatttcctg tgggtgctgg ggatccacgg
      781 acctaatacc acatcggcaa ttcgagaaac cgttttttct gaggctaatc tggagaatct
      841 ctcctgggcc gctcaacatg gcactacctg gggcgcgcca tatccgatta cctggacttc
      901 tattaatgat gcattcgcca actgcggcgg ttcaggtatg acgttggggt tattgttggc
      961 tatttttatc gcttctaagc gtgcggaata ccgtgatctg gcaaaaatgt catttatccc
     1021 cggtattttc aatatcaatg aaccgataat gtttgacctt cctattgtac ttaaccccat
     1081 catgatggtg ccgtttatta tggttcccat tgttaactgt gccattggtt acttctttgt
     1141 ttcgatggaa attattccac cggttgctta tgccgtgccc tggactacgc ccggaccttt
     1201 aattgctttc ctcggaaccg gggggaactg gctggcttta ctggttggtt ttttatgttt
     1261 aggtgtggcg acaatgatct atttaccttt tgttattgcc gccaacaaag tcaataacat
     1321 gacaactaac ggataattcc atttacccag gaaggagatt gtgatgtttg cagatgaaga
     1381 acttgttatg gagttactgg tcaacgcggg gcaggcacgt tctgacgcga tggaagccat
     1441 acgttgtgcc gggcaaaaag actggcaggg cgcaactcag ctaatggcca gttctgaatc
     1501 tgcctgtctt caggcacata agattcagac cgcattaatt agtcaggatg aaggctgtgg
     1561 caaaataaaa gttaatttaa ttctgatt
//
LOCUS       DAZGLJ010000427         1586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000427 DAZGLJ010000000
VERSION     DAZGLJ010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.437"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(480..686)
                     /locus_tag="ACHK49_005101"
     CDS             complement(480..686)
                     /locus_tag="ACHK49_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265551.1"
                     /translation="MNKEQSADEVSLDLIRVKNMLNSTISMSYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNSKDYSLVVD"
     gene            987..1397
                     /locus_tag="ACHK49_005102"
     CDS             987..1397
                     /locus_tag="ACHK49_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFO9265552.1"
                     /translation="MAQAAIFKEIFDQVRKDLNCELFYSELKRHNVSLYIYYLATDNI
                     HIVLENDNIVLVKGLKKVVNVKFSRNKHLIETSYNKLKSKEITFQQYRENLAKAGVFR
                     WVTNIQEHQRYYYAFDNSLLFTESIQKTTQILPR"
BASE COUNT          538 a          342 c          277 g          429 t
ORIGIN      
        1 caggtgaaaa gagaaataac ttttaattat caataaatta ccaaacatgc tgccgccgcc
       61 atggaaatgc aaaaactagc ctttttccgc gacgctcccg ccccgtggca ggggccccca
      121 ccgggaggac ccgacagcct gacagcctga cagccgtgat gagcatctga tacagcgctt
      181 tacataatgg cataggaata attcagaagg acatcacagc atgcccacac aaattagtgt
      241 gagtgtcctg tttcttccta cgcacaggac tgacgagcat gaggggaaaa tatgcgaacc
      301 ataaatgcct catcttcagc aatgcaaccg gcatccgaac aggacgatat tgtaaattca
      361 cctaattacg aggacattgc agtagttgaa ttgcagctct gtattagcct gacagtgaca
      421 gaatgcgata ttgactctgt cacaggtgaa atagtttgaa tgattagcag ttatggtgct
      481 cagtcaacca ccagggaata atccttcgaa ttcttatcgt gcttcaccaa cgctgcctca
      541 attgccctga atgcttccag agacacctga tgttctatac atgcaattac aacatccggg
      601 taactcatag aaatggtgct attaagcata ttttttacac gaatcagatc caacgagact
      661 tcatcagcag attgttcttt attcattttg tcgctccatg cgtttgctcg tcatctagcg
      721 gttaaaatat tacttcaaat cttactgcat aaaagaggaa aagtatgaaa gtttgagtac
      781 atcgacctta catacatctg tcggttgcat atccctcctg gatgccagca aggctcaatt
      841 ttgttacgca atcaacacta ttcattaaaa acaggcttaa tatttgacat aaatcatcaa
      901 caaaacacaa agaggtcaga ccagattgaa acaataaaca cgataatgct aactacgcgc
      961 catcgtatca catggaaggt ttaccaatgg ctcaggctgc catttttaaa gaaatattcg
     1021 atcaagtgcg taaagattta aactgtgaat tattttattc tgagctaaaa cgtcacaatg
     1081 tctcacttta tatttactat ttagccacag ataatattca cattgtgtta gaaaacgaca
     1141 acatagtgtt agtaaaagga ctcaaaaagg ttgtaaatgt taaattctcc agaaacaaac
     1201 atcttataga gacctcctat aataagttga aatcaaaaga aatcacattt caacaataca
     1261 gggaaaatct tgctaaagca ggagttttcc gatgggttac aaatatccag gaacaccaaa
     1321 gatattacta tgcctttgat aactcattac tatttactga aagcatccag aaaactacac
     1381 agatcttacc acgctaaacc ataacgtccg gcttctctca ctcctgagcc ggactgcatt
     1441 ggtttaataa aaaccatcaa caattgtgat ttagatattc ggaaccattc aaatataaca
     1501 aaaccccgaa aaaacgaggt ttatggataa attttattat tgaatacatc agattaaatt
     1561 aatcttgaca tcatagcttt caagac
//
LOCUS       DAZGLJ010000428         1575 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000428 DAZGLJ010000000
VERSION     DAZGLJ010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.438"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..400)
                     /locus_tag="ACHK49_005103"
     CDS             complement(<1..400)
                     /locus_tag="ACHK49_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="espD domain protein"
                     /protein_id="HFO9265553.1"
                     /translation="MLNVNSDIQSMRSGASAATATSGINQPEVTSALDLQLVKSTAPS
                     ASWTESTALPTPPAGHSLVTPSVAEDVLSKLFGGISGEVRSRTEGTEPQRSTQNASSG
                     YPYLSQVNNVDPQAMMMMVTLLSLDASAQKV"
     gene            complement(413..991)
                     /gene="espA"
                     /locus_tag="ACHK49_005104"
     CDS             complement(413..991)
                     /gene="espA"
                     /locus_tag="ACHK49_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFO9265554.1"
                     /translation="MDTSTATSVASANASTSTSTVYDLGSMSKDEVVQLFNKVGVFQA
                     ALLMFAYMYQAQSDLSIAKFADMNEASKESTTAQKMANLVDAKIADVQSSSDKNKKAK
                     LPQEVIDYINDPRNDITVSGISDLNAELGAGDLQTVKAAISAKSNNLTTVVNNSQLEI
                     QQMSNTLNLLTSARSDIQSLQYRTISAISLGK"
     gene            complement(1049..>1575)
                     /locus_tag="ACHK49_005105"
     CDS             complement(1049..>1575)
                     /locus_tag="ACHK49_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TyeA family type III secretion system gatekeeper
                     subunit"
                     /protein_id="HFO9265555.1"
                     /translation="QEGIQKIKKLYEKAKDEDSEKGASLLDWFMEIKDLPEREKHLKV
                     IIRALSFDLSYMSSFEDKVKTSSIISDLCRVIIFLSLDNYTDIIAISINKDKDVILNE
                     VLSIIEHVWLTEDWLLESPSRVSIVEDKHVYYFHLLKDFFASLPDACFIDREQRDNTL
                     LMIGKVIDYKEDVI"
BASE COUNT          467 a          347 c          285 g          476 t
ORIGIN      
        1 caactttctg cgctgaagca tccagggata acagggtgac catcatcatc atggcctgag
       61 ggtcaacgtt attcacctga gaaagataag gatagccaga gcttgcattc tgagtgctgc
      121 gctgtggttc ggttccctct gttcgactcc taacttcacc actaatacca ccaaacagtt
      181 tactcaggac atcctcagca acagagggcg tcactaatga gtggcctgcc ggtggcgttg
      241 gtaaagcggt actttctgtc caacttgctg atggggccgt ggatttaacc agttgtaaat
      301 ccagcgcgga agtcacttca ggctgattaa tacctgaagt agccgtagca gcgctggccc
      361 cagacctcat agactggata tcgctattta cattaagcat agttatctcc ggttatttac
      421 caagggatat tgctgaaata gttctgtatt gcagtgactg aatatcagaa cgtgcactcg
      481 ttaataggtt taacgtattt gacatttgct gtatttcaag ctggctatta ttcactaccg
      541 tggtcaagtt attcgatttg gccgaaatag cggccttcac cgtttgcaaa tcaccagcgc
      601 ctaattcagc atttagatcg ctaataccac ttactgtaat gtcattgcga ggatcattta
      661 tatagtcaat cacttcttga ggaagtttgg ctttcttatt cttgtcagaa ctactctgaa
      721 catcagcaat tttagcatcc acaagattag ccattttttg ggctgtggtt gactccttag
      781 atgcctcatt catatcagca aactttgcaa tcgacagatc gctttgtgcc tgatacatat
      841 aggcaaacat gagaagcgca gcctgaaaaa caccgacttt attaaatagc tgaactactt
      901 cgtctttcga catactgcct aagtcataga ctgtcgatgt cgaagtactc gcgttcgcac
      961 tagcaactga tgttgcagtt gatgtatcca tgtatatacc tcttaataat ctttcttagc
     1021 aagaccaaaa acgattaaaa aaccaatatc aaataacatc ctccttataa tcaattactt
     1081 taccaatcat taataatgta ttatctctct gttccctatc aataaaacaa gcgtcaggta
     1141 atgatgcaaa aaagtctttc aataggtgga aataataaac atgtttatct tcgacaatcg
     1201 atacccgaga agggctttcc aggagccagt cttctgttaa ccatacatgt tcaataatag
     1261 acaatacttc atttaaaatg acatctttat ctttattaat agagattgca ataatatctg
     1321 tatagttatc aagcgataaa aaaatgatta cccggcataa atcgctaata attgaagatg
     1381 ttttcacctt atcttcaaaa gaagacatat aagacagatc gaacgatagc gccctaataa
     1441 taacctttaa gtgcttctca cgctctggaa gatccttaat ctccataaac cagtcaagta
     1501 aagaggctcc cttttcagaa tcctcatcct tggccttttc ataaagcttt ttgatcttct
     1561 ggataccttc ctgat
//
LOCUS       DAZGLJ010000429         1573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000429 DAZGLJ010000000
VERSION     DAZGLJ010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.439"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..354)
                     /locus_tag="ACHK49_005106"
     CDS             complement(<1..354)
                     /locus_tag="ACHK49_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265556.1"
                     /translation="MPGLVSYISSTSFANEMAEMRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGLGLVYAELLNIGFNDGVDALRNRVLSVLPGMVAQRQENSLQAKISE
                     CTFTVDIEKLHCPGEV"
     gene            complement(645..1220)
                     /locus_tag="ACHK49_005107"
                     /pseudo
     CDS             complement(645..1220)
                     /locus_tag="ACHK49_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
     gene            complement(1333..1572)
                     /locus_tag="ACHK49_005108"
     CDS             complement(1333..1572)
                     /locus_tag="ACHK49_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO9265557.1"
                     /translation="MCGCGKHDGREHVQSLTAQLRLGPADILESDENGIIPEQDRVIT
                     QVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          436 a          439 c          267 g          431 t
ORIGIN      
        1 cacctcacca ggacagtgaa gtttttcaat atcaacagta aaggtacatt ccgatatttt
       61 ggcctgcaaa gagttctctt gtcgctgcgc caccattcca ggaagcacgc ttaacaccct
      121 gtttctgagc gcatcaactc catcattaaa accaatattc aataattctg cataaaccag
      181 tcccagccct tcactttctg ctaaaaaacg gcctgtatct ggcatataag aaactctaac
      241 tctctcccct cccaggagaa atcctccaat ctgcccttcc attacctgct gacgcatttc
      301 cgccatctca ttcgcgaatg aagtcgatga tatatatgat actaatccag gcattaccat
      361 attccttcat acagataaat atttcctctg ggcttcataa caaacatctc cctgacatga
      421 caacaaaaac cggagccgga ctccggtttt tgtgaatccg tcggctattt catcccgcca
      481 atatttcccc gtcagcacgc cagatttgca gcggcctcac cactcactgt atatgctttt
      541 tagctgcatc cagtacacca attaccacat ccttataccc tgaacatctg tagcatttca
      601 tataagcaac tggcagttaa catatagctt gggtaatatt tatttcactt atcctttatg
      661 acaaagtttc ctttggtttg gtcataaata cattgttctt gacttacaat cattgatgac
      721 gttattggtt ctcgtgttag cgggtgcttc ccaccgtcgc gaaccaaatg agaaaaagaa
      781 accgaatcaa ataaggtaca aaccaaagaa ccttctgaat ttttaacaaa cacaccttct
      841 tctggtttat ccagtataat tgggcatcga accgcctcgg atggacactg aaggctctcc
      901 gtattaacag aaaacttaca ttgtgatatt ttcccctcaa tatcaccatg tgattgtgtg
      961 ttacctgaac ctgaaaccat tcttaacatt atatccctga gcgcttcagg accaccatta
     1021 agccctgtaa gtaataatcc agaaagcaaa ctgttattgc ccccactcgc cagaaagcga
     1081 ccatccgttt cactatatgt gatacggacc gtctacccgc ctactgtaat ttctccaccg
     1141 ccactggcgg ccatttgagc acgaagaacc tgcattccgg tggaaaatga attggatgca
     1201 atagctgatg ttaacggcat gacaaaactc cctatttaaa tattcattcc aaacacaggg
     1261 agtcataaca aacagccccc atacatgaca acaaaaaccg gagccggact ccggttttgt
     1321 gaagctgtcg ggttacttca tcccgccaat attttcccac gtcccgtcag cacgcagaat
     1381 ttgcagcggt cttaccacac actgtatctg ctttttatcc gcatccagta tcaccacctg
     1441 cgtgattacc ctgtcctgct ccgggataat accattctca tcggactcca ggatgtctgc
     1501 cggccccagt cgcagttgtg ctgtaagcga ctgcacgtgt tcacggccat catgctttcc
     1561 gcaaccacac aga
//
LOCUS       DAZGLJ010000430         1571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000430 DAZGLJ010000000
VERSION     DAZGLJ010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.440"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            84..1475
                     /gene="narK"
                     /locus_tag="ACHK49_005109"
     CDS             84..1475
                     /gene="narK"
                     /locus_tag="ACHK49_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO9265558.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT          284 a          394 c          432 g          461 t
ORIGIN      
        1 atcttatcgt ttgatttaca tcaaattgcc tttagctaca gacactaagg tggcagacat
       61 cgaaacgagt atcagaggtg tctatgagtc actcatccgc ccccgaaagg gctactggag
      121 ctgtcattac agattggcga ccggaagatc ctgcgttctg gcaacaacgc ggtcaacgta
      181 ttgccagccg caacctgtgg atttccgttc cctgtctgct gctggcgttt tgcgtatgga
      241 tgttgttcag cgctgttgcg gtgaacctac cgaaagtcgg ctttaatttt acgaccgatc
      301 agctatttat gttgactgcg ctgccttcgg tttctggcgc gttattacgt gttccatact
      361 cctttatggt tcctatcttc ggtggtcgtc gctggacggc gttcagcacc ggtattctga
      421 ttattccttg cgtctggctg ggttttgccg tgcaggatac ctccacgcct tatagcgtct
      481 tcatcatcat ctctctgcta tgcggctttg ctggcgcgaa cttcgcctcc agtatggcaa
      541 acatcagctt cttctttccg aaacagaagc agggtggcgc gctgggtctg aatggtggtc
      601 tgggcaacat gggcgtcagc gtcatgcagt tggttgctcc gctggtggta tcactgtcga
      661 ttttcgcagt atttggtagc cagggcgtca aacagccgga tgggactgag ctgtatctgg
      721 cgaatgcgtc ctggatatgg gtgccgttcc ttgccatctt cactattgcg gcgtggtttg
      781 gcatgaacga tcttgctacc tcgaaagcct ccatcaagga gcagttgccg gtactcaaac
      841 ggggtcatct gtggattatg agcctgctgt atctggcaac cttcggctcc ttcatcggct
      901 tctccgcggg ctttgcaatg ctgtcaaaaa cgcagttccc ggatgttcag attctgcaat
      961 acgctttctt cgggccgttt attggtgcgc tggcgcgttc tgcaggtggt gcattatctg
     1021 accgtctggg cggaactcgt gtcacgctgg tgaactttat tctgatggcg attttcagcg
     1081 gcctgctgtt cctgacctta ccgactgacg ggcagggcgg aagcttcatg gcgttcttcg
     1141 cggtcttcct ggcgctgttc ctgacagctg ggctgggtag tggttccact ttccagatga
     1201 tttcagtgat cttccgtaaa ctgacaatgg atcgcgtgaa agcagaaggg ggttctgacg
     1261 aacgtgcgat gcgtgaagcg gcaaccgaca cggcagcggc gctgggtttc atctctgcga
     1321 ttggcgctat tggtggcttc tttatcccga aagcgtttgg tagctcgctg gcattaacgg
     1381 gttcgccagt cggcgcaatg aaggtatttt tgattttcta tatcgcctgc gtagtgatta
     1441 cctgggcggt atatggtcgg cattctaaaa aataaaccgt tactcgtcat acttcgggtt
     1501 acatgtgctg cggctgcgtt cattcacccc agtcacttac tttagtaagc tcctgggatt
     1561 cattcacttg c
//
LOCUS       DAZGLJ010000431         1551 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000431 DAZGLJ010000000
VERSION     DAZGLJ010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.442"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..490)
                     /gene="sctT"
                     /locus_tag="ACHK49_005110"
     CDS             complement(<1..490)
                     /gene="sctT"
                     /locus_tag="ACHK49_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFO9265559.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFN"
     gene            complement(492..752)
                     /locus_tag="ACHK49_005111"
     CDS             complement(492..752)
                     /locus_tag="ACHK49_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO9265560.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(761..1426)
                     /locus_tag="ACHK49_005112"
     CDS             complement(761..1426)
                     /locus_tag="ACHK49_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO9265561.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(1416..1551)
                     /locus_tag="ACHK49_005113"
                     /pseudo
     CDS             complement(1416..1551)
                     /locus_tag="ACHK49_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
BASE COUNT          543 a          340 c          239 g          429 t
ORIGIN      
        1 aattaaaatt gcctgaaggc cataaattat aactttgcca aagagtttcc agaataaagt
       61 tcattccacc tttagtaaga taaaccgcag cagagaaaag gttaaataat cttgctaatt
      121 ccgaggtatc gacacctgta gccggatcca aagtactact taatgttgca ccgcgttggt
      181 tatcgataaa gctacctata gcaaggaaaa tccaaaacgg ggatgctaat aagcatgcaa
      241 taaacagccc tatgataatt tctctggcag cgaacaaagc aatatcgatt tcatttaagt
      301 tcaataattc atagtggtaa tgctgaacta atgcacatga taccactatt attattgggt
      361 gacgaactac tgttggtata ttcccactgg cgaaaaatgg taacaaatag aaagttggtg
      421 ccaatcggca aaaaccgagc gctgtagcag ccagcagact atgcaactga tagagtatcg
      481 cctcccccat gctatcctct cgcgaatgct aaattcagca tttcgatacc aaatgaatat
      541 aatttttcac cataccaccc gctcattaaa aaaaagcaaa tacttacgca caataatttc
      601 acgccaaatg gcaacgtctg ttcttgcaac tgcgttaccg tttgaaaaag ccctaccaat
      661 aaaccaacaa atgtcgcaac tgcaataggt cctgcagaca taactaaaat cagatacagc
      721 gccctattcc ccgcaaaaac aatatcgtcc atattttttc tcagggatta atggataggt
      781 caaaatattg taaaatgagc ccttttgata acattgtcca accgtccatc gcgacaaaca
      841 aaattaattt tatcggtgta gatatggtta ccggactcat catcatcata cctaacgtta
      901 acagaacgct ggatataacc agatccacta cgacaaaggg taaatagata taaaaaccta
      961 taataaatgc actttttatc tcactcaatg catatgcggg cagtaacgag aaaattgaaa
     1021 tattatcatc atcgattatc tcttcattgt cttcagaact attcaccttc tgaatctttt
     1081 caaaaaagct aaccaactca ggttcagaat attttatcaa atatgattta taaccactca
     1141 tgcctgtctc aacaaaatta acaaccgatg ctacattatt gaatgaaaga ttctcattct
     1201 gactgttatt atatatttcc ttcccaaccg gcatcattac gaacattgac aatagcaaag
     1261 caaccccatt aagtgtcata tttgatggca cttgctgaag tcccagcgcg tttcggacaa
     1321 taacaaaaac aatcgagaat ttaataaaac atgtcccgga tgctattata aacggcaata
     1381 gtgtaaataa tgataatatc gctatcagcg aaatgctatt tgacattatt attccctgat
     1441 aaccaagaat gaatttctac gcccaattta tcatccactt caacaagttc gccatagcca
     1501 gttagcgcac catttacacg tatctctata ttttctcaga ttcaggaagc a
//
LOCUS       DAZGLJ010000432         1546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000432 DAZGLJ010000000
VERSION     DAZGLJ010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.443"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..374
                     /locus_tag="ACHK49_005114"
     CDS             <1..374
                     /locus_tag="ACHK49_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HFO9265562.1"
                     /translation="WQHHDVIDIKFIADIQFNTDLCGDTYISRGPVLYAIELESDILI
                     KKTLLNGKYFDSGYIPCSRADESIQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIV
                     RKLIPFGQTILRKVTFSNIES"
     gene            411..>1546
                     /locus_tag="ACHK49_005115"
     CDS             411..>1546
                     /locus_tag="ACHK49_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFO9265563.1"
                     /translation="MYLTDNIIKLSNKIGQRVYTVVSEMSIDAWITKEPQPFVYRRSG
                     AYQKLTIGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGI
                     TNKASSYGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIAT
                     CNQLLKSLYYDVEVLFDWINDGQKFESIHPKGIIPEKIITKRSERTDEIIRILEYIDN
                     TLITFSNEEIIKCQIAIQSIISKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRT
                     FATALTNIEKYPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECD
                     LNLVSGESLIRQIMHGTRFTLQNFSKKINYVWQPDVFGFPATLP"
BASE COUNT          492 a          282 c          303 g          469 t
ORIGIN      
        1 catggcaaca tcatgatgtg attgacatta aatttatagc tgatatacag tttaatactg
       61 acttatgtgg tgacacttat atttccagag gtcccgtttt atatgcaatt gagcttgaga
      121 gcgatatttt aatcaaaaaa actctgctga acgggaaata ttttgactca ggatacatac
      181 cctgtagtag agctgatgaa tctatccagt tctcttatgc tgatatggaa tctttttcat
      241 attcaacctc accagagggc aaacaataca ttgaaggctg tttttattta aacaagacta
      301 aaatagtcag aaaacttata ccatttggtc aaaccattct tcgcaaagta actttctcca
      361 atatagagag ttaatatttt cgagtattat aattaattaa agggatttct atgtacctta
      421 ctgataatat tatcaaactg tcgaataaaa taggtcagcg tgtttatact gttgtctctg
      481 aaatgtcgat tgatgcatgg ataacaaaag aaccgcagcc ttttgtttat cgccgtagcg
      541 gcgcttacca gaaactcacc attggctcag actggggtca actgtttgac tgtgcctgga
      601 tgcgagttta tggtaaacgt ccagcagata aatttagaca caaactcgtc gctctcgttg
      661 atatcggtgg cgaaggtctt attgtggata agaatggttc acccatctgc ggcatcacga
      721 ataaagcatc atcttatggc gtaccgccag ataaaccagg taaatgggtg gttgaccttt
      781 ctctcgttag tgaaaatgac gaggtcgagt tttggattga tgctgcgtgc aacgatttat
      841 ttggctatgt cactaatgga gggattatta ctgatgttca tatcgcgaca tgtaatcaac
      901 tactgaaatc cctttattat gatgttgaag tgcttttcga ctggattaat gatggtcaga
      961 agtttgaatc catacatcct aaaggtatta tcccagaaaa aattatcacc aaaagaagcg
     1021 agcgtactga cgaaatcata agaattctcg aatatattga taatacgtta attaccttta
     1081 gcaatgaaga aataattaaa tgtcaaattg caatccaatc cataattagt aaaaacaata
     1141 atccttcaga gttcaggatt atggctactg gtcatgccca tttagatata gcgtggatgt
     1201 ggcctttaag agaaggacgc agaaaagcaa taagaacatt tgccaccgcc ctgacaaaca
     1261 ttgaaaaata tcctgattac atttttggcg ctagtcagta ccaacttttt cactggataa
     1321 aaaaagatta tccctatttc ttcgaaaagt taaaaaaaca aatcgccgca ggccgctttg
     1381 agttacaagg ttgcttttgg gtcgaatgcg atttaaatct ggtaagtggt gaatcgctga
     1441 taaggcaaat catgcatggg acacgattca cactgcagaa tttttcaaag aagataaatt
     1501 atgtctggca accggatgtc tttggattcc ccgccacgct tccaca
//
LOCUS       DAZGLJ010000433         1527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000433 DAZGLJ010000000
VERSION     DAZGLJ010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.444"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1527
                     /locus_tag="ACHK49_005116"
     CDS             <1..>1527
                     /locus_tag="ACHK49_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO9265564.1"
                     /translation="VYDRSGKQVRSFTYDDKYRGRMVAHRHTGRPEICYRYDSDGRVT
                     EQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAGLKRVVKKEHADGSVTQSQFD
                     AVGRLKAQTDAAGRTTEYSPDVVTGLTTPDGRASAFYYNHHNQLTSATGPDGLELRRE
                     YDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFTDCS
                     GYVTRYDHDRFGQVTAVHREEGLSQYHAYDSRGQLTAVKDTQGHETRYEYNIAGDLTA
                     VIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLD
                     RLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWYYDEADRLTHRTVKGETAERWQY
                     DERGWLTDISHISEGHRVAVHYGYDSKGRLASEHLTVHHPQTEALLWQHETRHAYNAQ
                     GLAKRCIPDSLPAVEWLTYGSGYLAGMKLGDTPLVEYTRDRLHRETLRRFGRYELTTA
                     "
BASE COUNT          378 a          447 c          462 g          240 t
ORIGIN      
        1 ggtgtatgac cgtagtggca aacaggtgcg cagctttact tacgatgata aataccgggg
       61 ccggatggtg gcgcaccgtc acacgggccg accggaaatc tgttaccgtt acgacagcga
      121 cgggcgggtg acagaacagc taaacccggc aggcttaagt tacacgtatc agtatgaaaa
      181 agaccgcatc accatcaccg acagcctgaa ccgccgtgaa gtgctgcaca cgcagggcga
      241 agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca cgcagagtca
      301 gtttgacgcg gtgggcaggc ttaaggcaca gacggatgcc gcaggccgga caacagaata
      361 cagcccggat gtggtgacgg gcctcaccac gccggatggc agggcatcgg cgttttacta
      421 taaccaccac aaccagttaa cgtcagccac cgggcctgac gggctggaat tgcgccggga
      481 atatgatgaa tccggccgtc tgattcagga aactgcccct gacggcgata tcacccgcta
      541 ccgttatgat aatccacaca gtgacttacc ctgcgcaacg gaagatgcca ccggcagccg
      601 gaaaaccatg acgtggagcc gttacggtca gttgctgagc ttcaccgact gttccggtta
      661 tgtaacccgc tatgaccatg accgttttgg tcaggtgacg gcggtgcacc gcgaggaagg
      721 cctgagccag taccacgcat acgacagccg tggacagtta actgcggtga aagacacgca
      781 gggccatgaa acgcggtatg aatacaacat cgccggtgac ctgaccgccg tcattgcccc
      841 ggacggcagc agaaacggga cacagtacga tgcgtgggga aaggccatct gtaccacaca
      901 gggcgggctg acgcgcagta tggaatacga tgctgccgga cgggtcatcc gcctgaccag
      961 tgaaaacggc agccacacca ccttccgtta cgatgtactc gaccggctga tacaggaaac
     1021 cggctttgac ggccgcacac agcgttatca ccacgacctg accggcaaac ttatccgcag
     1081 cgaggatgag gggctggtca cccactggta ctatgacgaa gcagaccgcc tcactcaccg
     1141 caccgtgaag ggtgaaaccg cagagcggtg gcagtatgac gaacgcggct ggctgacaga
     1201 catcagccat atcagcgaag ggcaccgggt ggcggtgcac tatggttatg acagtaaagg
     1261 ccgcctcgcc agtgaacacc tgacggtgca tcatccgcag acggaagcac tgctctggca
     1321 gcatgagacc agacatgcgt acaacgcgca gggactggcg aaacgctgta taccggacag
     1381 cctgcccgcc gtggaatggc tgacctacgg cagcggttac ctggcaggca tgaaactcgg
     1441 cgacacaccg ctggtggagt acacccgcga ccgcctgcac cgggaaacgc tgcgccgctt
     1501 cggccgttat gaactcacca ccgctta
//
LOCUS       DAZGLJ010000434         1526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000434 DAZGLJ010000000
VERSION     DAZGLJ010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.445"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            190..351
                     /locus_tag="ACHK49_005117"
                     /pseudo
     CDS             190..351
                     /locus_tag="ACHK49_005117"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            335..400
                     /locus_tag="ACHK49_005118"
     CDS             335..400
                     /locus_tag="ACHK49_005118"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HFO9265565.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            516..773
                     /locus_tag="ACHK49_005119"
     CDS             516..773
                     /locus_tag="ACHK49_005119"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9265566.1"
                     /translation="MATFFAYPADIRKVIYTTNAIESLNSVIRHAIRKRKVFPTDESV
                     KKVVWLAIQAASQKWTMPLRDWRMAMSRFIIEFGDRLDGHF"
     gene            complement(820..1389)
                     /gene="nleF"
                     /locus_tag="ACHK49_005120"
     CDS             complement(820..1389)
                     /gene="nleF"
                     /locus_tag="ACHK49_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HFO9265567.1"
                     /translation="MLPTSGSSANLYSWMYVSGRGNPSTPESVSELNHNHFLSPELQD
                     KLDVMVSIYSCARNNNELEEIFQELSAFVSGLMDKRNSVFEVRNENTDEVVGALRAGM
                     TIEDRDSYIRDLFFLHSLKVKIEESRQGKEDSKCKVYNLLCPHHSSELYGDLRAMKCL
                     VEGCSDDFNPFDIIRVPDLTYNKGSLQCG"
     gene            complement(1455..>1526)
                     /locus_tag="ACHK49_005121"
     CDS             complement(1455..>1526)
                     /locus_tag="ACHK49_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265568.1"
                     /translation="EHQVMQSYHGGKLDLISVVLSKI"
BASE COUNT          452 a          353 c          302 g          419 t
ORIGIN      
        1 taataggcta tataatactg caagacacaa cacatgctgt cactatgagt caactattta
       61 gatgatatta gtgacctgtt tcagagcatt agcgcaaggc gattctttgt cttcttgcac
      121 taatttttta tcataaaaat gttcctagca ctgggcatca atatcgcagg tcagaaagag
      181 ctcctgggga tgcggctggc cgaaaatgaa ggggcgaatt tctggttcaa tgtgctgact
      241 gaactgaaaa accgcggtct gaacgatatc ctcatcgcct gtgtgtatgg cctgaaagaa
      301 ttcccggagg cccgcatcca gttatgcatc gtgcatatgg tgcgcaacag catgcgcttc
      361 gtgtcatgga aggaatacaa agccgtcact cgcgacctga aagcgattag cctcccacag
      421 aagaggcagg ccagcaggca ctggaagcgt ttgctgcggc ctgggactgc cgctatccgc
      481 agataagccg gtgctagctg tcaaactgga ctaacttggc gacgtttttc gcttatccgg
      541 cagatatccg caaagtgatc tacacaacga acgccatcga gtcgctgaac agtgtgatcc
      601 ggcatgccat caggaaacgc aaggtgttcc cgacggacga gtcagtaaaa aaagtggtgt
      661 ggctggcaat ccaggccgcg tcacagaaat ggacaatgcc gttaagggac tggcgaatgg
      721 caatgagccg ctttattatc gagtttggtg accgtcttga cggtcacttt taagaaaagg
      781 catttacaca gaatcctaaa caggctcaca ggcctctaat catccacatt gtaaagatcc
      841 tttgttgtaa gtaagatctg gtaccctaat aatatcaaaa ggattaaaat catcactgca
      901 tccttccacg aggcatttca ttgctcgtag atcaccatat agctctgaag agtgatgcgg
      961 acatagcaga ttataaactt tacatttcga atcttctttg ccttgtctac tttcctcaat
     1021 ttttactttc aatgaatgca gaaaaaaaag atccctgata taactatccc tgtcctctat
     1081 cgtcattccc gccctcagcg ctccgacaac ctcatcagta ttttcatttc tcacctcaaa
     1141 tacactattt ctcttatcca tcagcccact tacaaaagca cttagctctt gaaaaatttc
     1201 ctcaagctca ttattatttc tggcacatga atatatagag accataacat caagtttatc
     1261 ttgtaattca ggagaaagaa agtgattatg attaagctca cttactgatt ccggagtcga
     1321 agggttacct cttcctgata catacatcca tgaataaaga tttgctgaag aaccacttgt
     1381 tggtaacata tcaaaacccc cttaacaaat aaatccataa atattattgc aactaatata
     1441 tctggataaa aatattatat cttacttaat actacactaa taagatccag ctttcctccg
     1501 tgataagatt gcatgacttg atgttc
//
LOCUS       DAZGLJ010000435         1516 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000435 DAZGLJ010000000
VERSION     DAZGLJ010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.446"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1120
                     /locus_tag="ACHK49_005122"
     CDS             <1..1120
                     /locus_tag="ACHK49_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO9265569.1"
                     /translation="GVTDANYYPEGMHSQKRENWREYLERERGKKPGEEKHHQRKTTL
                     PMSVGSAGYDTQLDYVVKGIIPAVSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGRR
                     VAHGAVLYVVGEGGIGVPRRVKAWEVVHGEQVKNLYLVNRPIFPAAPLDVEEMVIAAR
                     QVERETGKPVRMIILDTLARCFGGNDENDSRDMGAFIRGCDKLKRRTGATVLVVHHSG
                     KDETKGARGSSAFRASLDAEYRIRREDAGSEALVISCTKMKDAEELKEAAYDLRVVEL
                     FTDADGELITSLVVVDKPRPPVELERIEEAGNKTENHAALWQCIRTRTAHGEACTIAL
                     LRDDMKKLGYEMKHFRRWLYKLENDGVIVIDGDDVRPL"
     gene            1322..>1516
                     /locus_tag="ACHK49_005123"
     CDS             1322..>1516
                     /locus_tag="ACHK49_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265570.1"
                     /translation="MNRKTRDKTAPKYKALDMTELSLKVAIRTIDRHAGEGYAKEHPE
                     LISAFMTTAAANFATLTEREI"
BASE COUNT          421 a          320 c          484 g          291 t
ORIGIN      
        1 gggtgtaact gatgcgaatt attaccccga agggatgcac agccagaagc gggagaactg
       61 gcgcgaatat ctggaacggg agcgggggaa aaagccaggg gaagaaaaac accaccagcg
      121 aaaaaccacg ctaccaatga gcgtgggatc tgccggatac gacacacaac ttgattacgt
      181 ggttaagggg attattccgg cggtatcgct gtgcagcata tacggggcga gcggttcata
      241 taaatcgttc cttgcgggtt cgtgggcgtg tcacgtttcc acgggccgcc agtggggagg
      301 ccgcagggtg gcgcatggcg ctgttctcta tgtggttggt gaaggcggta tcggcgttcc
      361 gcgtcgtgta aaagcctggg aggttgtgca cggtgagcag gtgaaaaatc tgtatctggt
      421 aaaccgtccc atctttccgg ctgccccgct tgatgttgag gaaatggtta tcgctgcccg
      481 tcaggtggaa cgggaaacgg gtaaaccggt tcgaatgatt attctggata cgctggcgcg
      541 ctgctttggc ggcaatgatg aaaatgattc ccgtgatatg ggggcgttta tccgtgggtg
      601 tgacaaactg aaacggcgca caggggcaac ggtgctggtg gttcaccatt ccggcaagga
      661 tgaaacaaag ggggcgcgtg gttccagtgc atttcgtgca tcactggacg ccgaataccg
      721 gatacgcagg gaggacgcag gcagcgaagc gctggttatc tcatgtacca aaatgaagga
      781 cgcggaggaa ctcaaagaag cagcatatga cttacgcgtg gtggagcttt ttaccgacgc
      841 tgacggggag ttaatcacgt cgctggtggt ggtggataag ccgcgccctc ccgttgaact
      901 ggagcgcatc gaggaggcag ggaacaagac ggagaatcac gccgcgctat ggcagtgcat
      961 cagaacacga acagcacacg gggaggcgtg cactatcgcc cttctccgcg acgatatgaa
     1021 aaagctgggg tatgagatga agcacttccg gcgctggctg tacaagctgg aaaatgatgg
     1081 cgtaatcgtt attgacggtg atgacgtgcg cccgctgtaa aaagtgggga gtaaaagtgg
     1141 ggggagtggg gaatttaaca aaattgaaac ataattaccc actttcccac ctgtatatac
     1201 ccaaaaaagt ggggagtaaa aaacatattg aaaaacataa cgttagaatc acaaaaaaaa
     1261 gaggtgggga gacggtgggt aatttcaaaa agtggggagc aaaagtgggg agtagtgggg
     1321 aatgaacaga aaaaccagag ataagacagc gccaaaatat aaagcgttag acatgacaga
     1381 gctttcctta aaggtggcaa tcagaacgat agaccgccac gcgggggaag gatacgcgaa
     1441 ggagcatccc gaactgataa gcgcattcat gaccacagcg gcggcaaact ttgccacgct
     1501 gacagaacgg gagatt
//
LOCUS       DAZGLJ010000436         1507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000436 DAZGLJ010000000
VERSION     DAZGLJ010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.447"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..335
                     /locus_tag="ACHK49_005124"
     CDS             <1..335
                     /locus_tag="ACHK49_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO9265571.1"
                     /translation="DFHREDDWWRNGQNLYLDNMAVTGFYRVPLSSAQAGDILLCCFG
                     ASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSVWRHRHWHASAFTGIYND
                     LAAASACM"
     gene            332..913
                     /locus_tag="ACHK49_005125"
     CDS             332..913
                     /locus_tag="ACHK49_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO9265572.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGYDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGTVVVVGRQG"
     gene            1256..>1507
                     /locus_tag="ACHK49_005126"
     CDS             1256..>1507
                     /locus_tag="ACHK49_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265573.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESS"
BASE COUNT          350 a          367 c          466 g          324 t
ORIGIN      
        1 cggattttca tcgcgaggat gactggtggc gcaacggtca gaacctttac ctggacaata
       61 tggcggtcac cggcttttac cgggtgcccc tgtcctctgc acaggcgggc gatattctgc
      121 tgtgctgctt tggtgcttcg gtaccgaacc atgccgccat ttactgcggc aacggtgagc
      181 tgcttcacca tctgcctgaa caactgagta aacgggagag gtattccgaa aaatggcaac
      241 gacgaacgca ttctgtctgg cgtcaccgcc actggcacgc atctgccttc acggggattt
      301 acaacgattt ggccgccgcc tcagcctgta tgtgaacacg gcagcggaag ccatccgggc
      361 gctgtcgtta caggtgccgg gctttcgccg tcagatgaac gaaggctggt accagatacg
      421 tattgccggt tatgacacgg caccggaggc ggtgtacgcc cgtcttcacg aacagctggg
      481 tgagggaacg gtcatccata ttgtgccgcg actggccggg gccggaaagg gtggactgca
      541 gattgtgctg ggggcggcag ccatcgtggg gtcgttcttc acggccgggg catcaatggc
      601 gttatggggt tcagccctgg cagccggtgg tttttctgcc accacaatgc tgttttcact
      661 tggagccagc atgattctgg gcggtgtggc ccagatgctg gccccgaagg caaaaacacc
      721 ggattaccgc gcaacggata acggcagaca gaacacgtac ttttcctcgc tggataacat
      781 gattgcccag gggaacccga tgccggtgcc ttacggggaa atgctggttg gctcccgccg
      841 tatatcccag gacatcagca cccgtgatga aggcgggggc ggaacggtcg tggttgtcgg
      901 gcgacaggga taaaacataa aaaaatcccg cagtgatcgc ggagctgcgg ggacagacaa
      961 atgaagatca atgttaagga gttgtttttg ttactcgggc aaaaaaacac taacgcagcg
     1021 aaattataag cgccacagtc agtgtgtgaa aatgtgaaga tattcagaat ttttatgcca
     1081 ttaccggttt taaccaacag gattatcggt gggcatgaaa gaaaaccccg gtatctgctg
     1141 ataccggggt ttctctttag catggcagaa atgtgtttca tgcttttcgg gcgaaggata
     1201 tccgacttct gtacggaatg gcaagtggcg gttaatttat tcaggggaag gctgtatggg
     1261 aaaaggtggc ggtaaggcac acacgcctcg tgaggcgaag gataatctca aatccacgca
     1321 gatgatgagt gtgattgatg cgattggtga gggaccgata gaaggtccgg tgaagggact
     1381 gcagagtatt ctggtgaaca aaaccccact gacggacacg gacggcaatc ccgtgataca
     1441 cggtgtgacc gcggtctggc gtgccgggga gcaggagcag acaccaccgg aaggctttga
     1501 gtcctcc
//
LOCUS       DAZGLJ010000437         1502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000437 DAZGLJ010000000
VERSION     DAZGLJ010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.448"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(55..459)
                     /locus_tag="ACHK49_005127"
     CDS             complement(55..459)
                     /locus_tag="ACHK49_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO9265574.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(680..1411)
                     /gene="bluR"
                     /locus_tag="ACHK49_005128"
     CDS             complement(680..1411)
                     /gene="bluR"
                     /locus_tag="ACHK49_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO9265575.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          401 a          335 c          321 g          445 t
ORIGIN      
        1 attatgctaa tgccttactg aattatgaag catttcttaa gcatccaact ttagctagat
       61 taatggttta ttattttcta catcttcaat atataaaagc gtattatcaa tggcgtagta
      121 actgcgtttg ttatgattaa catcagtaac ccaccggaaa acgcccgcgc ctgctagtgt
      181 tgaacagtat tcccgaaatg tagattttcc gcaaatatga agcaatgcgg cctcttttat
      241 tttagcaggg ttcttggtcg tactaacttt taacaggttc ctggttcctc ttaataacaa
      301 aaccgtgtca tcgtgagtaa taattctgat gttatccgta gccagataat aaatgtaatg
      361 tgcaatacgg tgatgtttta attctgaata aaaccaggag aagttttgct cttttctcac
      421 ttgctcaaac atcttttgaa aaacaacgac ctgatccatc aggataataa cctcttgtta
      481 gttgtgagac tgcgtagtgt gcacgatcgg ttttaccact tcaatctggt ctgtcctttg
      541 gctgtgatat gtacagagtg tgatagaggg aatatctgaa ttctcccggt gagcattttg
      601 caacggacca gctccggtac aaacgctgtt gtgggttcag attataacat tctgtctaag
      661 gggcgggata aaggtgaaat tagggggcat gaaagatgac tttataacct tgctcacccc
      721 aatgttgtaa aagttcgttt tgccttctcg ttggtgccat gcctgtccag acaatcaatg
      781 tttgcgtcgg gaacagttcc gggcgcggcg attcaatggg atcagcaaga acagaaatgt
      841 gccatcctga caatgataaa cgccatgctt ccagccacaa tcgtgctctc tcttcaacat
      901 tccacgccat cagaatggct tctttaccgg gcttacggcg catttcgaaa agcgaggttg
      961 ctgcgtattc aattaatgcg ccgtcaaaca tactgctcat aatgcgggag gtgttgtgat
     1021 caagcacgag acgctggcga acaggaaggt aaacatgatt aatcaattga tctactgggt
     1081 actctcgacc cagtgaaata attctcgcgc gtagtttggc aggattagcc atgcgaagaa
     1141 ttgacatcat ctcttcttgc aggcggctcc agtcatcttc cgtatcctgg ctggtggttt
     1201 ccagtaatgc tttaactttg cctacaggga cgccattact tatccaacgc ttgatctctt
     1261 cgatgcgttg tatgtcttct tcatcaaaga gtcggtgtcc gccttcactg cgctgtggtt
     1321 ttaacaaacc gtagcggcgt tgccaggccc ggagagtgac aggattaatc ccgcaacgtt
     1381 cagcaacatc accaatgctg taataagcca caattcctcc ttgcggtcac aaatctccgt
     1441 cgcctgtaca cgacccaata atactttgta caatatacgc taaaattgta caaagtataa
     1501 at
//
LOCUS       DAZGLJ010000438         1501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000438 DAZGLJ010000000
VERSION     DAZGLJ010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.449"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(38..340)
                     /locus_tag="ACHK49_005129"
     CDS             complement(38..340)
                     /locus_tag="ACHK49_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9265576.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSPSYLSQMASGRTAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            438..809
                     /locus_tag="ACHK49_005130"
     CDS             438..809
                     /locus_tag="ACHK49_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265577.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGVSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            830..1021
                     /locus_tag="ACHK49_005131"
     CDS             830..1021
                     /locus_tag="ACHK49_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFO9265578.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            1023..1301
                     /locus_tag="ACHK49_005132"
     CDS             1023..1301
                     /locus_tag="ACHK49_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9265579.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
BASE COUNT          454 a          341 c          331 g          375 t
ORIGIN      
        1 acattcatcg ccatgcggat gtgttcatgc ttgattttca tgaatcaact ccatcagata
       61 agcatgcact acaatcacct tcagcatgaa ctacatgtgt ttgccccaaa cgaatgccgc
      121 tcgcatactc aggccaaata agctcccaat catgaggtcg tagctccgcc ctacttactt
      181 ggccttccgt cgcagattcg atcataaggg cgcgggttgg agatatagct gttcgtccag
      241 acgccatttg cgataagtaa gatggcgata caccaagtct ggccgcgaat ttcttagcat
      301 caccaaccct taatgattta ataaactctt ttaatgtcat accttcctcg gtttagtgtt
      361 tttttgcaag tttagtgttt aataaaccat taagtcaagt atttgcttgt ttagtgatta
      421 ctaaagataa ttaccacatg caaaaaaaag aaattcgccg tttacgtctc aaggagtggt
      481 ttaaagataa aactctgcca cccaaagaga agagctacct atctcaacta atgagtggga
      541 gagcctcgtt tggagaaaag gctgccagaa gaatagagca aacatacggg atgccggaag
      601 ggtatctgga tgcggaatac gcagaacaac cgggggtttc tccaccacat gcagggttaa
      661 cgtctaatca actggaatta ttgcagattt tttcagcctt ccctgaggat gagcaacgcc
      721 agataatcag cgagttaaag cagaaaaaag aatcaatgga agatctcata gcgagatgga
      781 ttgcggcgca aaaatgccgc cgcgcctgag ttataaaacc ggaggaaaca tgaatagagc
      841 cctttcacca atggtttctg aatttgaaac cattgaacaa gaaaacagtt acaacgaatg
      901 gctgcgtgcg aaagtagcaa cgagccttgc agatccgcgc ccagcaattc cccatgacga
      961 agttgagcgc agaatggcag aacgctttgc taagatgcgc aaggaacgga gcaagcagta
     1021 aaatgttacc cgtgttatgg cttgaaagcg cagataccga cctagatgat ataactagtt
     1081 atattgctcg tttcgacata gatgcggctg aacgcttatg gcagcgatta aggggttgtg
     1141 tgctgccgtt atccgaacat ccgtatttat acccaccaag cgacagagta cctggcttgc
     1201 gtgagattgt agcccaccct aactatataa ttctataccg cgtaacaaca tcaagcgttg
     1261 aagtagtaaa cgtgatccac gcaagacgcc agtttcccta actttcacta ccaatagaaa
     1321 cataacaacc gcaacgactt tatcaaaagc gttgtgtttg ttatgtcccg cggtttagtt
     1381 tttacttgac ttaagtttaa tgtttattaa actaaaaata ccaccccgcc ccacagaacg
     1441 cagggaaata cttcgagtta cccggcagtg gtcaggggtt aagtagccag cccgaggcgt
     1501 a
//
LOCUS       DAZGLJ010000439         1490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000439 DAZGLJ010000000
VERSION     DAZGLJ010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.450"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            59..634
                     /locus_tag="ACHK49_005133"
                     /pseudo
     CDS             59..634
                     /locus_tag="ACHK49_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
     gene            726..1216
                     /locus_tag="ACHK49_005134"
                     /pseudo
     CDS             726..1216
                     /locus_tag="ACHK49_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
     gene            1216..1365
                     /locus_tag="ACHK49_005135"
     CDS             1216..1365
                     /locus_tag="ACHK49_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO9265580.1"
                     /translation="MLIAWLQDNIDCESGIIFDNDEDKTDSAALLPCIEQAREARYFP
                     SVLSA"
BASE COUNT          460 a          324 c          334 g          372 t
ORIGIN      
        1 actcactcca ttagaattat catgaactca catttaaaga aactaagaag gaggttgtat
       61 gtttagtata agatctcagc tacctataag cgccagcgta tcaattccgg cagaacaatc
      121 tcaatccatc tccacaattc ctggagggag aaaaatagag acagtgccag agaaagaagg
      181 attgcttgtg tttttaggaa caaaatccgt aggtgaatat gctcttaata ttcttggtca
      241 aaatgtttca agagtcacaa cagggaaaaa accttatgat atattattcc tgcacgaagc
      301 tacaaaacaa gattttgata aaaagaaaac agagtttacc tttcctggtg caaacagaag
      361 ttatctacaa tcaagtaaca ccgatgttgc tgccgcagca gctataagta ttgcggcaac
      421 agagatgaaa actatcctac caaaggattt aacaccagaa aaatacaata aaatttatct
      481 gccaggggat ggttctgctg gcctacctct tcttaagtgt ggagatgaat ttttatcacc
      541 aactgatatc gtcaatcgtc tggtagaaca taatcttcat gaagtagaag acatcagatt
      601 aacatcctgt cactcagcca acataacaaa aaactagact ggccccctga atctccagac
      661 aaccagtatc acttaaataa gtgatagtct taatactagt ttttagacta gtcattggag
      721 aacagatgat tgatgtctta gggcctgaga aacgcagacg gcgtaccaca caggaaaaga
      781 tcgcaattgt tcagcagagc tttgaaccgg ggatgacggt ctccctcgtt gcccggcaac
      841 atggtgtagc agccagccag ttatttctct ggcgtaagca ataccaggaa ggaagtctta
      901 ctgctgtcgc cgccggagaa caggttgttc ctgcctctga acttgctgcc gccatgaagc
      961 agattaaaga actccagcgc ctgctcggca agaaaacgat ggaaaatgaa ctcctcaaag
     1021 aagccgttga atatggacgg gcaaaaaagt ggatagcgca cgcgccctta ttgcccgggg
     1081 atggggagta agcttagtca gccgttgtct ccgggtgtcg cgtgcgcagt tgtacgtcat
     1141 tctcagacga accgatgact ggatggatgg ccgccgcagt cgtcacactg atgatacgga
     1201 tgtgcttctc cgtatatcct cattgcgtgg ctgcaggaca atatcgactg cgaatcggga
     1261 attatctttg acaacgatga ggataaaacg gattcggcag cactgctgcc ctgtattgaa
     1321 caggccaggg aagccaggta ctttccttct gtgctctcag cctgatcccg agtgtgattc
     1381 cctttcgata tcaaaaggga ctgcgggtat aattgagggt gcatttttaa tcaaaccaaa
     1441 caaattgcat taatatcaat atgataaagg aaattttcga ttcccgagtc
//
LOCUS       DAZGLJ010000440         1488 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000440 DAZGLJ010000000
VERSION     DAZGLJ010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.451"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            45..134
                     /locus_tag="ACHK49_005136"
     CDS             45..134
                     /locus_tag="ACHK49_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265581.1"
                     /translation="MPINLTSYLWVQGQKVVPAVVFSNFTLVD"
     gene            154..>1488
                     /gene="nleL"
                     /locus_tag="ACHK49_005137"
     CDS             154..>1488
                     /gene="nleL"
                     /locus_tag="ACHK49_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFO9265582.1"
                     /translation="MLPTTNISVNSGVISFESPVDSPSNEDVEVALEKWCAEGEFSEN
                     RHEVASKILDVISTNGETLSISEPITTLPDLLPGSLKELVLNGCTELKSINCLPPNLS
                     SLSMVGCSSLEVINCSIPENVINLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNES
                     QCQFLAYDVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNL
                     SSVNFSGAVLDDTDLRMSDLSQAVLENCSFKNSILNECNFCYANLSNCIIRALFENSN
                     FSNSNLKNASFKGSSYIQYPPILNEADLTGAIIIPGMVLSGAILGDVKELFSEKSNTI
                     NLGGCYIDLSDIQENILSVLDNYTKSNKSILLTMNTSDDKYNHDKVRAAEELIKKISL
                     DELAAFRPYVKMSLADSFSIHPYLNNANIQQWLEPICDDFFDTIMSWFNNSIM"
BASE COUNT          471 a          229 c          293 g          495 t
ORIGIN      
        1 tgttagataa aaaagaggag ataattcaat agggagttaa attaatgccg ataaatctga
       61 catcttattt gtgggtacag ggacagaaag ttgtcccggc agttgtgttt tctaatttta
      121 ctttagtgga ctaagtaaaa aggagtgaga taaatgctgc ccactacaaa tatctctgta
      181 aattctggag taatatcttt tgaaagtcct gtagattcac catctaacga ggatgttgaa
      241 gttgccctcg aaaagtggtg cgctgaggga gaatttagcg aaaatcgtca tgaggttgca
      301 tcaaaaatac ttgatgttat aagtactaat ggagagactt tatcaatcag tgagccaata
      361 acaacattac cagacttgct tccaggttct ctgaaagaac tggttttgaa tggatgtaca
      421 gagcttaaat caataaactg cttacccccc aacttatctt cattaagtat ggttggatgc
      481 tcatcattag aggttataaa ttgcagcata cctgaaaatg tcattaattt atctttatgc
      541 cattgtagtt ctttgaaaca tatagaaggt tcctttcctg aggcactcag aaattccgta
      601 tatttaaatg gctgtaattc attaaatgaa tcgcaatgtc aattccttgc atatgatgtc
      661 agtcaaggcc gtgcctgcct gagcaaagct gagcttactg ctgacttaat ttggttgtcg
      721 gctaaccgaa cgggtgaaga gtctgctgaa gaattgaatt actctggatg tgacttgtca
      781 ggtctaagtc ttgtagggct gaatttatca tcagtaaatt tttctggagc agtgcttgat
      841 gatacagatc tcaggatgag tgatttgtct caggctgtat tggaaaactg ttcttttaaa
      901 aactcgattt tgaatgaatg taatttttgt tatgctaatt tatctaattg tattattagg
      961 gctttgtttg aaaactctaa tttcagcaat tccaatctta aaaatgcatc atttaaagga
     1021 tcttcatata tacaatatcc tccaattttg aacgaggctg atttaacagg agctattata
     1081 attcctggaa tggttttaag tggtgctatc ttaggtgatg taaaggagct ctttagtgaa
     1141 aaaagtaata ccattaatct aggagggtgt tacatagatc tatctgacat acaggaaaat
     1201 atattatctg tgttggataa ctatacaaaa tcaaataaat caattttatt gactatgaat
     1261 acatctgatg ataagtataa ccatgataaa gtaagggccg ctgaagaact tatcaaaaaa
     1321 atatctcttg acgaattagc ggcgttccgg ccctatgtta agatgtcttt ggctgattca
     1381 tttagtattc atccttattt gaacaacgca aatatacagc aatggctcga gcctatatgt
     1441 gatgactttt ttgatactat aatgtcttgg tttaataatt caataatg
//
LOCUS       DAZGLJ010000441         1483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000441 DAZGLJ010000000
VERSION     DAZGLJ010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.452"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..153)
                     /locus_tag="ACHK49_005138"
     CDS             complement(<1..153)
                     /locus_tag="ACHK49_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO9265583.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGASSLSVPQ
                     QQVQFDA"
     gene            complement(143..>1483)
                     /locus_tag="ACHK49_005139"
     CDS             complement(143..>1483)
                     /locus_tag="ACHK49_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9265584.1"
                     /translation="ISYRPNWRWLGMRETAAKSFVDEVEAAWSEYAEGMSGEIDVEGK
                     RTFTEFIREGVGVHAFNGEIFVQPVWDTESTQLFRTRFKAVSPKRVDTPGHGMGNRFL
                     RAGVEVDRYGRAVAYHICEDDFPFSGSGRWERIPRELPTGRPAMLHIFEPVEDGQTRG
                     ANQFYSVMERLKMLDSLQATQLQSAIVKAMYAATIESELDTEKAFEYIAGAPQGQKDN
                     PLINILDKFSTWYDTNNVTLGGVKIPHLFPGDDLKLQTAQDSDNGFSALEQALLRYIA
                     AGLGVSYEQLSRDYSKVSYSSARASANESWRYFMGRRKFIASRLATQMFSCWLEEALL
                     RGIIRPPRARFDFYQARSAWSRAEWIGAGRMAIDGLKEVQESVMRIEAGLSTYEKELA
                     LMGEDYQDIFRQQVRESAERQKAGLSRPVWIAQAYEQQIAESRRPEEETTPRET"
BASE COUNT          336 a          471 c          350 g          326 t
ORIGIN      
        1 agcatcaaac tgtacctgct gttgtggtac cgaaagactt gatgccccca tctcgcgccc
       61 gagcgcgcaa aagaaaaccc gcgcataggc gggctccaga agcagcggtt cattgaatgc
      121 tgcggcaata atgtgtgaaa gattacgtct cacgtggtgt tgtctcctct tccggcctgc
      181 gactctccgc tatctgctgc tcatacgcct gcgctatcca caccggacgt gagagtccgg
      241 ctttttgtcg ctcagcagat tccctgacct gctggcggaa aatgtcctga taatcctcgc
      301 ccatcagcgc cagctctttc tcatacgtgc tcagtccggc ctcaatgcgc atcactgatt
      361 cctggacttc cttgagcccg tcaatggcca ttcttccggc tccaatccac tcagcccgtg
      421 accaggctga tcgcgcctga taaaaatcaa aacgcgcccg tggcggacga ataatccccc
      481 gaagaagtgc ctcttccagc cagcaggaaa acatctgcgt ggccagccgg gacgcaataa
      541 attttcgtcg tcccataaaa tagcgccacg actcattggc ggaggcgcgg gcacttgaat
      601 aactgacctt cgagtaatcc cgggacaact gttcgtagga aacgccaaga ccggcggcga
      661 tataccgcag cagcgcctgt tcaagcgccg aaaatccatt gtctgaatcc tgcgcagtct
      721 gcagtttcag atcatcaccg gggaaaaggt gcggaatttt gacaccgccc agcgtcacgt
      781 tattcgtgtc ataccaggtg gagaacttat ccagaatatt aataagcgga ttatccttct
      841 gcccctgcgg cgcaccggcg atatattcaa aggccttttc ggtatcaagt tcactttcaa
      901 tcgtcgctgc atacatggct ttcacaatgg ccgactgaag ctgtgttgcc tgcagggaat
      961 ccagcatctt cagccgttcc atgacgctgt aaaactgatt agccccacgg gtctgcccgt
     1021 cctccaccgg ctcgaaaata tgcagcatgg ccggacgccc ggtgggtagt tcacgcggga
     1081 tccgttccca tcgtccacta ccagagaacg gaaaatcatc ctcacagata tggtacgcaa
     1141 cggcacggcc atatcgatcg acctccaccc cggcccgcag aaaacggttc cccataccgt
     1201 gtcctggcgt gtccacccgt ttcggactca cggctttaaa acgcgtacga aacagttgcg
     1261 tgctctccgt atcccagacc ggctgcacaa agatttcgcc gttaaacgca tgaacgccca
     1321 caccttcacg gataaattcc gtaaacgtgc gtttcccttc cacgtcgatc tcgccagaca
     1381 tcccttcggc gtattccgac caggccgcct ccacctcatc gacaaaactt tttgctgcag
     1441 tctcccgcat ccccagccag cgccagttcg gacggtagct gat
//
LOCUS       DAZGLJ010000442         1480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000442 DAZGLJ010000000
VERSION     DAZGLJ010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.453"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1480
                     /locus_tag="ACHK49_005140"
     CDS             <1..>1480
                     /locus_tag="ACHK49_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO9265585.1"
                     /translation="TVNLIKSARVGYTKMLLGVVGYFIEHKSRNSLLFQPTDSAAEDF
                     MKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLGGAAAKNYREKSV
                     DVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIKGTCQIEKAANES
                     AHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEHHGCVIHQSELDQ
                     SNGRWICENTGMWTRDGLTFFSARGDEIPPPRSITFHIWTAYSPFTTWVQIVYDWLDA
                     LKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPARVVYLTAGIDSQR
                     NRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYRHADGTEMTISRV
                     CWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDT
                     AKEILYARMKADPSPADEATSYAIRFPDDPEIFSQTEAQQLVA"
BASE COUNT          343 a          358 c          436 g          343 t
ORIGIN      
        1 gcactgttaa tctgattaaa tctgcccgtg ttggctatac aaagatgttg ctgggggtgg
       61 tcgggtattt tattgagcat aaatcccgaa acagtctgct ttttcagccc acggattctg
      121 ccgctgaaga ttttatgaag tctcacgtgg aggcgacgat tcggaacgtg ccatgcctga
      181 aagacctttc cccatggctg ggtcgtaaac atcgtgacaa tactctcacg ctgaaacgct
      241 tttcatcggg cgtcggtttc tggtgcctgg gcggcgctgc cgccaaaaac taccgtgaaa
      301 aatccgtgga cgtggtctgc tatgacgaac tttcctcgtt cgagccggat gtcgaaaaag
      361 agggctcgcc aaccctgctg ggggataagc gtattgaggg gtcggtgtgg ccaaaatcca
      421 ttcgcggctc gacgcctaaa atcaaaggca cctgccagat cgaaaaagcc gctaacgagt
      481 cggcgcattt tatgcgtttt tatgtgccct gcccgcactg tggggaggag cagtatctga
      541 aatttggcga tgagtccacg ccttttgggc ttaaatggga gaaggacagc cctgaaagtg
      601 ttttctacct ctgtgaacat catggctgcg tgatccatca gtctgaactg gaccagagca
      661 acgggcggtg gatctgtgaa aacacgggca tgtggacccg tgacggtctg acgtttttca
      721 gcgcccgggg tgatgaaatt ccgccgccgc gctccatcac gttccatatc tggacggcgt
      781 acagtccgtt caccacctgg gtacagattg tctatgactg gctggatgca ctgaaagatc
      841 ccaacggcct gaaaaccttt gtgaacacca cgctgggcga gacctgggaa gaggccgtgg
      901 gcgaaaaact cgatcaccag gtactgatgg ataaggtggt gcgttacacg gcggcggtgc
      961 ctgcccgggt ggtttatctg acggcgggca ttgactcgca gcgaaaccgt tttgagatgt
     1021 atgtctgggg atgggctccg ggagaggaag cctttctggt ggataaaatc atcattatgg
     1081 ggcgtcctga tgaggaagag acgctgttac gtgtggatgc ggcgatcaac aaaaaatacc
     1141 gccatgcgga tggcaccgaa atgactattt cccgtgtctg ctgggacacc ggggggatcg
     1201 atggtgaaat tgtttatcag agatcaaaaa aacacggtgt tttccgggtg ctgccggtaa
     1261 aaggcgcatc tgtctatggc aagccggtga tcaccatgcc aaaaacccgc aatcagcggg
     1321 gcgtgtatct gtgtgaagtg ggaacggaca ccgcaaaaga aattctctat gcccgtatga
     1381 aagccgatcc ctcgcctgcg gatgaagcca cgtcgtatgc catccgtttt cctgatgatc
     1441 cggagatttt ttcgcagaca gaggcgcagc aactggtggc
//
LOCUS       DAZGLJ010000443         1464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000443 DAZGLJ010000000
VERSION     DAZGLJ010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.454"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..270)
                     /locus_tag="ACHK49_005141"
     CDS             complement(<1..270)
                     /locus_tag="ACHK49_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001760115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265586.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTS"
     gene            complement(365..571)
                     /locus_tag="ACHK49_005142"
     CDS             complement(365..571)
                     /locus_tag="ACHK49_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9265587.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGGFPAPIKM
                     ERSSRWLKSEVEAWLQARIAQSRP"
     gene            1328..1453
                     /locus_tag="ACHK49_005143"
                     /pseudo
     CDS             1328..1453
                     /locus_tag="ACHK49_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
BASE COUNT          379 a          340 c          413 g          332 t
ORIGIN      
        1 actggtgcat aaattcacta tcagatcatc atccaggggc ggtgtggccc gtttgtcagg
       61 gccataaatc agccggtgca ggctctgggg tacgatggca atctccggtt tttcaccatt
      121 gaatgacacc caaccgggcg tcagattatg gaagtagtag atattcggat gacacacgcc
      181 ccaggatacg gcgtgccggg tgaagccggt cagccatttc tggtagcttt cgtggagcca
      241 ttcgtagcgg gtctgatact gttgtttcat tttcatattc ctcacgggga tataacaggc
      301 agcagaatgc cgccggcagg ccggagatta cgtctgctgc gggtgaacgg ataacggtca
      361 gaaattacgg acgggactgt gcaatacgcg cctgcagcca ggcttccact tcacttttca
      421 gccagcggga gctgcgctcc attttgatgg gggcaggaaa gcccccatct ttgatgagtt
      481 tgtaaaacca cttatcggtt aagccggtaa gttgagtgat aaacgccatg tcgaccatct
      541 ggtcatccat cagcgaaaca ggggtagcca tgagaatgtt cctccggatg ttgttaaacc
      601 agccggtgtg gtcatgaaaa cagcgcgcca ccttcactgg cataacaacc ggggagaaac
      661 gtaaaaaata atcgggaatg tggctgtccg gagataaacg gggggattgc gatggcagag
      721 ccgtagcgga atcccgggtg gttgttgcga aaagatgctt ccgcacgcac gcgcgataaa
      781 ggagggagga ctttcgtgag acaaggggaa aactagcgtt ctgataattg cagagttgtt
      841 ttcggaggtg gttacagtga gtcagcccgt tcctggcgag gaggagactg atatcaccga
      901 tgggataagg ctgtctgatt ggtattataa atataagaag tcatcagaga gtaacgtaaa
      961 ggatttgcca gagtaagcta agagagtaaa tctctccggt aacagagcgc tgtgaatcgt
     1021 tcatgtatca gagtgcagat acatgaacgc tgatgcgtaa aatcagcagt gacacgccag
     1081 ctccccctgt gcccattcct gtataagaca tgaactgagc gtattaatgc tcctgccctg
     1141 agactgagcc ggtgagtgag gggctgagaa atccctcttg acactgctct aaaccagaaa
     1201 tacttccccg gttgttgcgg tgcgtccgga tccccgataa cggaacacta aggctgtatt
     1261 gcgggacacc tagtaagtgg cagcccgtaa tacgtcagga gacgcgggaa aagtgaattc
     1321 ccacacgcgt tataaactta cccttgcgga gctggaagcc tttaactctg ccgttgacaa
     1381 ccggctggca gaactgacaa tgaacaaact ttacgatcgc gcgccggctt ccgtctggaa
     1441 atatgtcacc tgaatattga agca
//
LOCUS       DAZGLJ010000444         1463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000444 DAZGLJ010000000
VERSION     DAZGLJ010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.455"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1463
                     /gene="tir"
                     /locus_tag="ACHK49_005144"
     CDS             <1..>1463
                     /gene="tir"
                     /locus_tag="ACHK49_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFO9265588.1"
                     /translation="GHNPNVRALIPPAPPLPSQTDGAGGARNQLINSNGPMGSRLLFT
                     PIRNSVADAADSRASDIPGLPTNPLRFAASEVSLHGALEVLHDKGGLDTLNSAIGSSL
                     FRVETRDDGSHVAIGQKNGLETTVVLSEQEFSSLQSLDPEGKNKFVFTGGRGGAGHAM
                     VTVASDIAEARQRIIDKLEPKDTKETKEPGDPNSGEGKIIEIHTSTSTSSLRADPKLW
                     LSLGTIAAGLIGMAATGIAQAVALTPEPDDPITTDPDAAANTAEAAAKDQLTKEAFQN
                     PDNQKVNIDENGNAIPSGELKDDVVAQIAEQAKAAGEQARQEAIESNSQAQQKYDEQH
                     AKREQEMSLSSGVGYGISGALILGGGIGAGVTAALHRKNQPAEQTITTRTVVDNQPTN
                     NASAQGNTDTSGPEESPASRRNSNASLASNGSDTSSTGTVENPYADVGMPRNDSLARI
                     SEEPIYDEVAADPNYSVIQHFSGNSPVTGRLVGTPGQ"
BASE COUNT          407 a          336 c          380 g          340 t
ORIGIN      
        1 tggccacaat cccaatgtga gagctttaat tccacctgca ccgccattac cttcacaaac
       61 cgacggtgca ggaggtgccc gtaatcagct cattaactca aatggcccga tggggtctcg
      121 tttgctattt acgcctataa ggaattctgt tgctgatgct gctgattctc gtgccagtga
      181 tattcccgga cttcctacaa atccactgcg ctttgctgcg tccgaggtat ctttgcatgg
      241 tgcgcttgaa gttcttcatg ataaaggggg gcttgatact cttaactctg ctattggatc
      301 ttcgttattc cgtgttgaaa ctcgggatga tggcagccat gttgctatcg ggcaaaaaaa
      361 tggcctcgag accactgttg ttttaagtga gcaagagttt tctagcttac agtcccttga
      421 tcctgaaggt aaaaacaaat ttgtatttac tggaggccgc ggtggcgcag ggcatgctat
      481 ggtcacggtt gcttcagata tcgccgaagc ccgtcagagg ataatagata aattagaacc
      541 aaaggataca aaggagacga aggagccagg ggatccaaat agtggcgagg gaaaaatcat
      601 tgaaattcat acctcaacct caacttctag cctccgtgca gatcctaaac tttggttgtc
      661 attggggact attgctgcag gtctgatagg gatggctgcg acggggattg cacaggctgt
      721 tgcgttgact ccagagccgg atgacccaat cactaccgac cctgatgctg cagcaaacac
      781 agctgaagca gcggcaaaag atcagttaac gaaagaagca ttccagaacc cagataacca
      841 gaaagttaat atcgatgaga acggaaatgc aattccgtcc ggggaactaa aagatgatgt
      901 tgttgcgcaa atagcagaac aagctaaagc ggcgggtgaa caggccagac aggaagctat
      961 tgaaagtaat tctcaggcgc agcaaaaata tgatgaacag catgctaaac gcgaacagga
     1021 aatgtctctt tcatcggggg ttggctacgg tattagtggt gcgctgattc ttggcggggg
     1081 aattggtgcc ggtgttactg ctgctcttca tcggaaaaac caaccggcag aacaaacaat
     1141 cactacacgt acggtagtcg ataatcagcc tacgaataac gcatctgcgc agggcaatac
     1201 tgacacaagt gggccagaag agtccccggc gagcagacgt aattcgaatg ccagcctcgc
     1261 atcgaacggg tctgacacct ccagcacggg cacggtagag aatccgtatg ctgacgttgg
     1321 aatgcccaga aatgattcac tggctcgcat ttcagaggaa cctatttatg atgaggtcgc
     1381 tgcagatcct aattatagcg tcattcaaca tttttcaggg aacagcccag ttaccggaag
     1441 gttagtggga accccagggc aag
//
LOCUS       DAZGLJ010000445         1448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000445 DAZGLJ010000000
VERSION     DAZGLJ010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.456"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..221)
                     /locus_tag="ACHK49_005145"
     CDS             complement(<1..221)
                     /locus_tag="ACHK49_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO9265589.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLKR
                     GNDGQPPWFEHDQMTVAAKILLRCTWLDPA"
     gene            407..1375
                     /locus_tag="ACHK49_005146"
     CDS             407..1375
                     /locus_tag="ACHK49_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO9265590.1"
                     /translation="MSQPGSLCVDIDVSKAILDIAASSAIEQFSVGNDSDGFDAIIAE
                     LRMYAIALVLMKATGGLETAIVCALQAEGFELAVVNPRQARDFARAMGYLAKTDSIDA
                     RVLSQMAEVLNRHPERERFIRALPDTECQVLTAMVVRRRQLITMLVAERNRLHPAHPL
                     NRKSINIIIKALEDELARLDADMNSHIHSHFKELAKRLSSIKGVGTMTAAALLAEVPE
                     QGNLSRREISVLVGMVPVNRDSGTMRGRRTIFGGRAGVRTALYMAALVATRFNPVIKV
                     FYMRLLAAGKPKKVALVTCIRKLLTILNAMLRKNEEWNESYHHVAP"
BASE COUNT          363 a          355 c          380 g          350 t
ORIGIN      
        1 gccgggtcaa gccaggtgca tcgcagcagg attttcgcag caacagtcat ctggtcatgt
       61 tcaaaccacg gcggttgtcc gtcatttccc cgcttaagtg ttaacggaca accacaggac
      121 acacagcaca aaacgtcctg tcggttgtgc ttcgcggctt ctttcgccgt aacaaaatgg
      181 cctttgctgt cattcgccat atagcagttc agaattcgca tatctcctcc ttgtatcttg
      241 acaattggtt aaaataactg tgattagatt tggcaaggtg tatgcagccc gatccctcaa
      301 ggggttcata gcccgtagct cagcgaatgg cgcatacact gagccactca tctgaaaccc
      361 gaacagttgt cagtacgcca taacgcattt gcaaggatgg gtaatcatga gtcagccagg
      421 ttcactttgt gtggatatcg atgtttcgaa agcgatactg gatattgccg ccagcagcgc
      481 tattgaacaa ttttctgtcg gcaatgacag tgatggcttt gacgccatca ttgctgagtt
      541 gagaatgtac gccattgcgc tggttttaat gaaagccact ggaggacttg aaaccgccat
      601 agtctgtgcg cttcaggcag aaggttttga gcttgccgtg gttaacccca gacaagctcg
      661 tgactttgcc cgcgccatgg gatacctcgc gaaaactgat agtattgatg ccagagtgct
      721 ttcccagatg gctgaggttc tcaaccggca tccggagcgg gaacgcttta tcagagcact
      781 gccggatact gaatgtcagg ttctcactgc aatggtagtt cgtaggcggc agttgattac
      841 gatgctggtt gccgagagaa accggttgca ccctgctcat ccattgaaca ggaaaagcat
      901 taatatcatc atcaaagcac tggaagacga gcttgcgcga ctagatgctg acatgaacag
      961 ccatatccat agccacttca aagagcttgc taagcgactg agcagtatta aaggtgttgg
     1021 caccatgacg gctgctgcgc tgctggcgga agtacccgaa cagggtaacc tctccaggcg
     1081 tgaaatcagt gtgcttgtcg gtatggttcc agtaaaccgg gactcgggca cgatgagagg
     1141 gcgacgaacc atctttggcg gcagggctgg tgtgcgaaca gccctgtaca tggcagcgct
     1201 tgtcgcaacc cgctttaacc cggtaataaa ggtgttttac atgcgactgc ttgccgcagg
     1261 aaaacccaaa aaagtggcgc tggttacctg tatacgcaag ctgctgacta tcctgaacgc
     1321 aatgctcaga aagaacgaag aatggaatga atcgtaccat cacgttgctc cataattttt
     1381 acgttcaaga cagttgctga gctgtccggg gaagactgaa acctccggtc tggcagtgtg
     1441 tgcagtac
//
LOCUS       DAZGLJ010000446         1440 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000446 DAZGLJ010000000
VERSION     DAZGLJ010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.457"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            48..329
                     /locus_tag="ACHK49_005147"
     CDS             48..329
                     /locus_tag="ACHK49_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFO9265591.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            326..598
                     /locus_tag="ACHK49_005148"
     CDS             326..598
                     /locus_tag="ACHK49_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265592.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFAELVAMKASALMKDMSMRSGVIKGNEFNQQG"
     gene            603..896
                     /locus_tag="ACHK49_005149"
     CDS             603..896
                     /locus_tag="ACHK49_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265593.1"
                     /translation="MSKVRVIFEFKHVSHDEKPAGNDCVEVHEKIGVDVKTERDTNNR
                     PTSLCGVYASILQYHSPEIIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ"
     gene            908..1438
                     /locus_tag="ACHK49_005150"
     CDS             908..1438
                     /locus_tag="ACHK49_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO9265594.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGNPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
BASE COUNT          417 a          292 c          419 g          312 t
ORIGIN      
        1 gaaggccacc cgcatcagcg gctgcacggt ttactgggag gtgtgaggtg aaaaaaaatc
       61 tcattgcatg ggcgtggtcg agtggtctta ttgagtttgg ttacgtcctg ccggaaggtg
      121 cattgccgat agttgccgga aagcctgcca cggtacggca tgtgattgag gttatggcgc
      181 gtcatggacg tgatgaacag gagcagttac tggttccggg gataccggaa gcggtgacgg
      241 aggaagaagc ctttaatgcc atgattcggt tctgccgtga ggtcagacgc cgggtcagtt
      301 atccaaacag aacgaggacc agaggatgag taaagtcgta cgcattattt tcgaatacaa
      361 ggagcacgtt atccataaaa acgctgatgg aacagtgcgc atgggggtaa gtctggacat
      421 acgttcaacc gggataaagc agaaaggtga tggacccgcc atgatttttg gggtggttat
      481 gctcgcggaa agcagagact ttgctgaact tgtggcaatg aaagccagtg cgctcatgaa
      541 agatatgagc atgcgttccg gggttattaa aggtaatgaa tttaatcagc aggggtaatt
      601 ccatgagcaa agtacgcgtt atttttgaat ttaagcatgt ttcgcatgac gaaaaaccgg
      661 caggcaatga ctgtgttgaa gtgcatgaaa agattggtgt ggatgtaaaa acagaacgtg
      721 atacgaataa caggccgacg tcactctgtg gcgtttatgc aagtattctc cagtatcaca
      781 gtcctgaaat tattcagttt ctctcagcgg aatttcaggc atctgtacag gcttttgggg
      841 cggatgccat cattaaacgc caccgcgtgc ataaagcatc aggcacactg caataaggaa
      901 aaacaaaatg gctaaacgtg ttacaaaatt aaaggccgca gccgaggctg caccgcagac
      961 ccgcgaagaa gtcagccgcg atatccgtac cctgggcgat attcagcgag aggcgctacg
     1021 gctggaaacg gcgatgaatg atgaagtggc agaaatcacc gcccgttata cgccgcagat
     1081 tgaaaacctt aaaaaagaaa tcaaagtgct ttttaagggg attcaggact ggtgcaaaac
     1141 caaccgcgat gagctgacga acggcggcaa aaccaaaaca gccaatctga ccaccggaac
     1201 agtgtcatgg cggctgggaa atccatcatg cagcgtcagc cgtgatgtgg aaggtgtgat
     1261 tgaaatgctg cgccgtatgg ggcttgagcg attcatccgc acgaaagagg aagtgaacaa
     1321 ggaagcggtc ctagcggagc cggatgcagt gaaaggcatt gccggtatta aggtgaataa
     1381 aggcgctgaa agtttttatg tcgagccttt tgaacaggac gccggactga ataaataaca
//
LOCUS       DAZGLJ010000447         1436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000447 DAZGLJ010000000
VERSION     DAZGLJ010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.458"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(207..347)
                     /locus_tag="ACHK49_005151"
     CDS             complement(207..347)
                     /locus_tag="ACHK49_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9265595.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(538..798)
                     /locus_tag="ACHK49_005152"
     CDS             complement(538..798)
                     /locus_tag="ACHK49_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO9265596.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASAVHSSEGIPWHELPASHRGNNQMSLPLPQN"
     gene            1031..>1436
                     /locus_tag="ACHK49_005153"
     CDS             1031..>1436
                     /locus_tag="ACHK49_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HFO9265597.1"
                     /translation="MGKNSQRRRDNKIKRENEKKRNLAAKINKMLYIKPDIFFDESGN
                     TGGNLLDPLQPVFTLSSSSISKQDALKALELTGSKSPTEAHFKTLRRRKSGQDGIIRL
                     LESKYVNEENVKIYLVDKKYMLTAKIVDILIET"
BASE COUNT          400 a          292 c          335 g          409 t
ORIGIN      
        1 acatttagtg gggggagggg agtaagcccg ctacattgtg ggctttgtct tatagtctga
       61 tcactgtttc aagtcggtga ggacaccaaa aagaaaaccc gcagttttta tgctgcgggt
      121 ttgtttcatg tctgtgagat agagtgcctt atcgacctta ccctggcaac cgattgacgg
      181 gggattgctc ccccgtcgcg gtttccttac tgcttacact gtaagaacgc cgcaaactcc
      241 gctccccaga agctcatccg tatttcgcac agtgaaccgt gcagcatcca gatgatgagg
      301 attgccgtca cgcagaacgt gatggccgta agcgattttt gcgacatagc acttgctcct
      361 tttccggaga ggcgctaacc tttcacttgt caaggtaatg cggttagggc ctcggttaaa
      421 cagagatgtt ttccggggcc tttccacatc tggccttcgg gtattccctc cgaccatcag
      481 ccgaaaggca cccgcgcgta atctatcgct tttttgttaa tccggcaatt ctgcctgtta
      541 gttctgaggt aaaggcaaac tcatctgatt gtttcccctg tgtgaagctg gcagctcatg
      601 ccacgggata ccttctgaag agtgaacggc ggaggcgtgt tttgatgtga atttatggaa
      661 ggcttccagt gttgagaagc atacgccgca ttccaggttg ttacactggt aatacttttg
      721 ccgcacggtg tttgaatcat tttccggacg acttgtgcgg atacgggcag atgcgccaca
      781 aagcggacaa cggaacatag agacctccct taacgtggtg cttctgttat tttaagttct
      841 actagctccg attccaactg actaaaccgc tttgagttga ggggctgaca ttcttattat
      901 tcgcttatca aagaatagct agtcaaacct gatacattaa aatcagcacg ttattttgat
      961 tgtgtgatat cataacatta tgttattaat gcgttaaatt gggatatcta attcagattg
     1021 tgaggtattt atgggaaaga atagtcaacg taggcgagat aataaaatta agcgagaaaa
     1081 tgagaaaaaa agaaacttag cggcaaagat aaataaaatg ttgtatataa agcctgatat
     1141 attttttgat gaatcaggta atactggcgg aaatttactc gatccattgc aacctgtttt
     1201 taccttaagt tcaagtagta ttagtaaaca ggatgcgctt aaggcattag aactaaccgg
     1261 aagcaagtct ccaacagaag cccactttaa aactcttaga cgtagaaaat ctggtcagga
     1321 tggcatcatt aggcttcttg aaagcaaata tgtcaatgaa gagaatgtta aaatatactt
     1381 ggtcgataaa aaatacatgc taacagcaaa gattgttgat attttgatcg agacgt
//
LOCUS       DAZGLJ010000448         1414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000448 DAZGLJ010000000
VERSION     DAZGLJ010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.459"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            241..>1414
                     /locus_tag="ACHK49_005154"
     CDS             241..>1414
                     /locus_tag="ACHK49_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9265598.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTSDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIAQYCDQTVPDGFGGTEPRMTFNAYLSQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSA"
BASE COUNT          347 a          359 c          435 g          273 t
ORIGIN      
        1 aaaagcgaaa aaatcccgca gtgctcacgg acaggaactg cgggagcgtt acgaagattg
       61 agtgtaagga attattctta tgtcacgaca aaaaacatta acgcagagaa attattagcg
      121 ccacagtcag tttgtgaaaa tgtgaagata ttcagaattt ttattcagtc atgatacagg
      181 catcctccgg gatgcctgtt gtttttgtgc gtaacagtta tcacagtaaa gggtgagaca
      241 atgggcaaag gtggcggcaa ggcgcacacg ccggtagagg caaaggacaa tcttaagtcc
      301 acgcagatga tgagcgtgat tgatgccatt ggtgaagggc cgattgaagg cccggtgaag
      361 gggctgcaga gtatcctggt gaacaaaacc ccgctgacgg acacggacgg caatcctgtg
      421 atacacggtg tgaccgcggt ctggcgcgcc ggggagcagg agcagacacc accggaaggc
      481 tttgagtcct ccggagcgga aaccggactg ggcgtggaag tgacgaaggc aaagccggta
      541 acgcgcacca ttacatccgc gaacattgac cgcctgcggg tcaccttcgg ggtgcagtca
      601 ctgttggaga ccacctcaaa gggcgaccgt aatccctctt ctgtccgact gctgattcag
      661 ttgcagcgta acggtaactg ggtgacggaa aaggatgtca ccattaacgg caagaccacc
      721 tcgcagtttc tggcgtcggt gattctggat aatctgcctc cccgcccctt taacatccgg
      781 atggtcaggg agacggcgga cagcacctcg gaccagctgc agaacagaac gctgtggtcg
      841 tcatacaccg aaatcatcga tgtgaaacag tgctacccga acacggcgat tgtggggctg
      901 caggtggatg cggagcagtt cggcggtcag cagatgacgg tgaactacca tatccgcggt
      961 cgcatcatcc aggtgccgtc aaactacgac ccggaaaaac gcacttacag cggtatctgg
     1021 gacggcagcc tgaaaccggc atacagcaac aacccggcct ggtgcctgtg ggacatgctg
     1081 actcacccgc gctacggcat gggaaaacgt ctgggggcgg cagacgtgga caaatgggcg
     1141 ctgtatgcca ttgcgcagta ctgcgaccag acggtcccgg atggcttcgg gggcacagag
     1201 ccgcggatga cctttaatgc atacctgtca caacagcgta aggcgtggga cgttctcagt
     1261 gatttctgct cggcgatgcg ctgtatgccg gtatggaacg gccagacgct gacgttcgtt
     1321 caggaccgcc cgtcggatgt ggtgtggccg tacaccaaca gcgatgtggt ggtggatgat
     1381 aacggcgtgg ggttccgcta cagcttcagt gccc
//
LOCUS       DAZGLJ010000449         1408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000449 DAZGLJ010000000
VERSION     DAZGLJ010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.460"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            221..868
                     /locus_tag="ACHK49_005155"
     CDS             221..868
                     /locus_tag="ACHK49_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO9265599.1"
                     /translation="MPINLTPYLTASGGLGEIPQDTLSGIRTLAFNGGAQVQMGSTIV
                     TIRSVFLGFFMGSVSPEGLSERALRTALNNVNRLERDLNGGLSGRQILASIPSPYASP
                     PRPSVMQTELVLEQIEKCSFNVNSSSLRATEEALTCPITLCIPEHGVIMRNAGDSDVC
                     TLYDKESLKHLVNTSSPHPLSREKITESMIVKENSCYFDFTSGSIKNVRGEITRL"
     gene            complement(1128..1347)
                     /locus_tag="ACHK49_005156"
                     /pseudo
     CDS             complement(1128..1347)
                     /locus_tag="ACHK49_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          413 a          238 c          327 g          430 t
ORIGIN      
        1 ggatgaagta acccgacagc ttcaaaaaac cggagtctgg ctccggtttt ttgttgtcat
       61 gtcatggtga tgtttgttaa tgaagttaaa tttattttgg cttgataatg gagtcttatc
      121 ttttgcaaga ctgataatgg tgtgtgaaag cattgtgata agtaagatgc ttcaacagga
      181 aatcaagtaa aatgttaatg aagttaatgg gggttttatt atgccaataa atttaacacc
      241 atatctaaca gcgagtgggg gactggggga aataccgcag gatactcttt ctggcatacg
      301 aacactggct tttaacggag gagctcaggt tcagatgggg agcactattg ttacaatacg
      361 ttctgtcttt ttaggcttct ttatgggaag tgtaagccct gaaggattat cagagagagc
      421 tttacgaact gcattgaata atgttaatcg tcttgaacgc gaccttaatg gagggctatc
      481 cggccgccag attttagcaa gtatacccag cccgtatgct tctccacctc gtccttctgt
      541 aatgcaaaca gagttggtgt tagagcaaat agaaaaatgc tcatttaatg ttaactcgtc
      601 atctctgagg gcgactgaag aggcattaac atgcccgatt acattatgca tccctgagca
      661 tggtgtgatt atgagaaatg ctggagattc agatgtatgt actttatatg ataaagaatc
      721 attaaaacat cttgttaaca caagttcacc tcatcctctg agcagagaaa aaataacaga
      781 gtcaatgatt gttaaggaaa actcgtgcta ttttgatttc acatcaggaa gtattaaaaa
      841 tgttcgtggt gagataacta gactataaga tgttagaaca tattgactga taagtgtaat
      901 tcacagacat cttttcagtt ttttctgtta attttctaat tgatggactg tatacaggga
      961 taaaaggaga gggtattgtc tggacatttc tgttagcata attggaaatg tgcgtgaaaa
     1021 aaagcataac agacatcatg tgttatgtat gtattcagag tgcccataat tcaccagtgc
     1081 agggataaat tgtaaaaaaa tcgcagtacg tcgtatgcag aacggtgcgg cgtacgcagt
     1141 tccagtggac cgtcgcctgt gataacggtc tttgtggaat aaccgttgcg ggagttagct
     1201 cctgatctgg actgattttt ctcattccca agatggtgtg tcatctctgc attgagagcg
     1261 gcttcaacgc tgagcttttt cagcaaccga tcaaactgac tgaggtcttc tgagggggct
     1321 gaggtttttg gccagttcgt tagcaataca ccagaaaaat tctaaacgag cctgtacata
     1381 gatttgtgta attgcctgat tttgatat
//
LOCUS       DAZGLJ010000450         1402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000450 DAZGLJ010000000
VERSION     DAZGLJ010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.461"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..854)
                     /locus_tag="ACHK49_005157"
     CDS             complement(<1..854)
                     /locus_tag="ACHK49_005157"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO9265600.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRV"
     gene            complement(918..>1402)
                     /locus_tag="ACHK49_005158"
     CDS             complement(918..>1402)
                     /locus_tag="ACHK49_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFO9265601.1"
                     /translation="RKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLD
                     HIGIDPSGVGQILDSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPL
                     MAWCVGNARVEPKGNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI
                     "
BASE COUNT          286 a          456 c          357 g          303 t
ORIGIN      
        1 acacggatca ccggcgcaga agccaccgcg gccacattcc cgttaccggc ctgtttcacc
       61 ggctgcgccg tggcggcctt accggcttcc agttcacgca ggcgcttcag gtgcgcatcc
      121 acctgacgga tttccgctgc ggtgttgtcg taatgctctt cctcctccac atccagcgtg
      181 cgcccttcct ctgcggcttt ggtcatgacc tcctcaaggg aggctgccag cgctgcacgc
      241 ttgttttcaa aacttttaat ctgttcgcca atattcatta tggtcttttc cttatgaaaa
      301 acggttgttg actgtgccgc agcgccggca gaagatgcga ttttcaccac cggtttccgg
      361 ttgccggacg cggcagaaaa cgggcggtcg taagatttaa tggtccggat ggtgcattcc
      421 gcattcgcgg gcacggtgac ggcagacacc tccatcagtt cccagcgcag aaaatgcagt
      481 ccgcctccgt ccagaaaggt gtattcatgg ggacggaagc ccacggacag ccccctgacc
      541 agcccggtct taatggccgc ccagacctca tccagccggg cagccagttg cgacggcata
      601 tccggtacgg gcttcaccag tgttgccgtg atttccagcc cttcgctgac ccggcgcacc
      661 gtacactgcc ccaccgggcg ggaatggtca tgctgccaga gaaacggtat cgcactgcca
      721 aactccgcgc cctccggctc caggatgtca ccatcccgat ccggagaagg cgttgacgca
      781 atcccggtga tcacccgttc atcctcactg aaggatttca ccgtcagcag ggaacaggcc
      841 cgtttaagag tcacatcagc ctcctgaaaa taaaaaaacc gccggagcgg ttcgtgatgg
      901 ttacagtgtg aacagggtta tatgaaaaaa accgcatatt ctttcttttt cggttccggg
      961 ttaagggaca tcagggagac cgcattgaac agcgccatca gcgggtcaat ttttccccgt
     1021 ccactggcct gtttggtgat aagaatggcg ttacctttag gctccacccg ggcattgccg
     1081 acacaccagg ccatcagggg ctggtcacca tgcaccagca ccccttcagc cagtttgcgc
     1141 tcggtggttt taatggcccc gcccagtttc cagccctggc ttatccccac cacaattccg
     1201 tcggggatcc cggcttccgc cagtgaatcc agaatctgcc ccacccctga cgggtcaata
     1261 ccgatatggt ccagtaactc agcctcatga atgcgacgca catattccgc cacttccgcc
     1321 gtgtcatccc cgacacgccg gacaatggtc atatctccac aggcaacaag atcctgaaac
     1381 cgggacgcct cgctcttccg tc
//
LOCUS       DAZGLJ010000451         1401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000451 DAZGLJ010000000
VERSION     DAZGLJ010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.462"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..854)
                     /locus_tag="ACHK49_005159"
     CDS             complement(<1..854)
                     /locus_tag="ACHK49_005159"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO9265602.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSTIPFLWQHDHSRPVGQCTVRRVREGLEITAMLVKPEPGMPSQMAARLDEAWAAIK
                     TGLVRGLSVGFRPHEYTYLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGASAQSITSFHKEKSAMNTGEQIKSFENKRAALAASLEEIMN
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELETSKAATAQPVKQAGNGNVAT
                     VASAPVIRV"
     gene            complement(918..>1401)
                     /locus_tag="ACHK49_005160"
     CDS             complement(918..>1401)
                     /locus_tag="ACHK49_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFO9265603.1"
                     /translation="RKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLD
                     HIGIDPSGVGQILDSLAEAGIPDESVVGISQGWKLGGAIKTTERKLAEGVLIHGDQPL
                     MAWCVGNARVEPKGNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKAYEVFFI
                     "
BASE COUNT          291 a          453 c          349 g          308 t
ORIGIN      
        1 acacggatca ccggcgctga agccaccgtg gccacattcc cgttaccggc ctgtttcacc
       61 ggctgtgccg tggcggcctt actggtttcc agttcacgca gacgcttcag gtgcgcatcc
      121 acctgacgga tttccgctgc ggtgttgtcg tagtgctctt cctcttccac atccagtgtg
      181 cggccttcct ctgcggcttt gttcatgatc tcctcaaggg aggctgccag cgccgcacgc
      241 ttgttttcaa aacttttaat ctgttcacca gtattcattg ctgacttttc cttatgaaaa
      301 gaggttattg actgtgccga agcgccggca gaagatgcga ttttcaccac cggtttccgg
      361 ttgccggacg cggcagaaaa cgggcggtcg taagatttaa tggtccggat ggtgcattcc
      421 gcattcgcgg gcacggtgac ggcagacacc tccatcagct cccagcgcag aaaatgcagt
      481 ccgcctccgt ccagataagt gtattcatgg ggccggaagc ccacagaaag ccccctgacc
      541 agcccggtct taatggcagc ccaggcctca tccagccggg ctgccatctg ggagggcatc
      601 cccggctccg gcttcaccag cattgccgtg atttccagcc cttccctgac ccgacgcacc
      661 gtacactggc ctacagggcg ggaatggtca tgctgccaga gaaacgggat cgtactgcca
      721 aactccgccc cctccggctc caggatgtca ccatcccgat ccggagaagg cgttgacgca
      781 atcccggtga tcacccgttc atcctcactg aaggatttca ccgtcagcag ggaacaggcc
      841 cgtttaagag tcacatcagc ctcctgaaaa taaaaaaacc gccgcagcgg ttcatgatgg
      901 ttacagggtg agcagggtta tatgaaaaaa acctcatacg ctttcttttt cggttccgga
      961 ttcagggaca tcagggacac cgcattgaag agcgccatca gcgggtcaat ttttccccgt
     1021 ccgctggcct gtttggtgat aagaatggcg ttacctttag gctccacccg ggcattaccg
     1081 acacaccagg ccatcagggg ctgatcaccg tgaatcagca ccccttcagc cagtttgcgc
     1141 tcggtggttt tgatggcccc gcccagcttc cagccctggc ttatccccac cacactctca
     1201 tcggggatcc cggcttccgc cagtgaatcc agaatctgcc ccacacctga cgggtcaata
     1261 ccgatatgat ccagtaactc agcctcatga atacgacgca catactccgc cacttccgcc
     1321 gtgtcatccc cgacccgacg gacaatcgtc atgtctccac aggccacaaa atcctgaaac
     1381 cgggatgcct cactcttccg t
//
LOCUS       DAZGLJ010000452         1398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000452 DAZGLJ010000000
VERSION     DAZGLJ010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.463"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..707
                     /locus_tag="ACHK49_005161"
     CDS             3..707
                     /locus_tag="ACHK49_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFO9265604.1"
                     /translation="MLLCGCKQQELLNHLDQQQANDVLAVLQRHNINAEKKDQGKTGF
                     SIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKARLYSAIEQRLE
                     QSLKIMDGIISSRVHVSYDVDNGDSGKTALPIHISVLAVYEKDINPEIKINDIKRFIV
                     NSSASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLALISVATCWLL
                     WKYRAILTNLVRLKIK"
     gene            723..1304
                     /locus_tag="ACHK49_005162"
     CDS             723..1304
                     /locus_tag="ACHK49_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFO9265605.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNTPINNPVLRSITNEMIVLQ
                     YNLSVEHFNLNSSLIYYINNWNLFPLFCLFSGYHFYRERFAERGFFYKVPAVLRDYLS
                     AIPVKINEKARYKPGIASYHNIITCGFSTLSPYIRQQPLAMQQRFNLLFPDFVDHIQL
                     PLPLASTLLERITFYAKKNRDELDKISCKWCCD"
BASE COUNT          459 a          237 c          256 g          446 t
ORIGIN      
        1 tcctgctgtt gtgcggctgc aagcagcagg aattattgaa ccatcttgat caacagcaag
       61 ctaatgatgt ccttgctgtt ttacaaagac ataatattaa tgctgaaaag aaggatcaag
      121 gcaaaacagg attttcaata tttgtcgaac caacggattt tgcttctgcg gtggattggc
      181 taaaaatcta caatctaccg ggtaagcctg atattcaaat atcgcagatg ttccctgcag
      241 atgcactcgt ttcatctcca cgagcggaaa aagcaaggct atattctgct attgagcagc
      301 ggttagagca atctctcaaa attatggatg gcattatttc ttcgagagta catgttagtt
      361 acgatgttga taatggcgat agtggtaaaa ctgctttacc aattcatatt tctgtacttg
      421 cagtatatga aaaagatatc aacccagaaa taaaaataaa tgatatcaag agatttattg
      481 ttaatagctc tgcaagcgta caatatgaaa atatttctgt tgtattgtca aaacgaagag
      541 acattattga gcaagcacca acatatgaaa ttagcgaacc tgtttttgct tatgataagg
      601 ctatgccggt ttctatctta cttgcattaa tatccgtagc gacttgctgg ctgttatgga
      661 agtatagggc cattctaact aatttagtca ggttgaaaat taagtagaca tatgagagcg
      721 ttatgaatct tgccctaaga aagattatat atgatccaat tagttatatt catcctcaac
      781 gagtaagctt gaataataca cctattaaca atccggtgct tagatctatt accaatgaaa
      841 tgattgttct gcagtacaat ctttcagttg aacattttaa tctgaatagt tcacttattt
      901 actacataaa caattggaat ttatttccat tgttctgttt attcagtggt tatcactttt
      961 atcgagaaag gtttgcagaa cgcggatttt tttataaggt tcctgctgtg ctaagggatt
     1021 atttatcagc tataccggtg aaaataaatg aaaaggcacg atacaaaccc ggcatcgcta
     1081 gctatcacaa tatcatcaca tgtggttttt caacgttgtc accctatata aggcaacaac
     1141 cattggcaat gcagcaacga tttaatttat tatttcctga ttttgttgat cacatacaat
     1201 tgccgttacc acttgcatca actcttttag agagaattac attttatgcg aaaaaaaata
     1261 gagatgagct tgataaaatc tcctgcaaat ggtgttgtga ttaaaagaaa aatttctgat
     1321 ggattgaaat aaatagtttc tcttaaagaa agtatccttc ttgaaaccag cgcaaaaatt
     1381 cagagcattg aggtaaag
//
LOCUS       DAZGLJ010000453         1384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000453 DAZGLJ010000000
VERSION     DAZGLJ010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.464"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(51..248)
                     /locus_tag="ACHK49_005163"
     CDS             complement(51..248)
                     /locus_tag="ACHK49_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO9265606.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            complement(480..1022)
                     /locus_tag="ACHK49_005164"
     CDS             complement(480..1022)
                     /locus_tag="ACHK49_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO9265607.1"
                     /translation="MIYPEITGKSGEHLRLKTLEAVWIQGKLRMWGRWSYIGGGKPGN
                     MFNQLLASKKLTKTAINEALRRIRESGIDKPELEAFLREMIAGRQKSWLSHCTDAEAL
                     RIDGVISKALARYPGLIDILRQRYEGRGMSKRKMAELLNEVHPEWCFSTCEKRIANWL
                     AVAEYALYIPMRESFAQKMS"
     gene            complement(1031..>1384)
                     /locus_tag="ACHK49_005165"
     CDS             complement(1031..>1384)
                     /locus_tag="ACHK49_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9265608.1"
                     /translation="IEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQRL
                     KLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQRV
                     PGGRLGVKIYKIESE"
BASE COUNT          344 a          385 c          277 g          378 t
ORIGIN      
        1 aggtgccgct aacacccacc agccgcccat taccacaaat taaaaagcct tcactgcgga
       61 aggcgtctgt aacaaccgaa ctgataatct gccagacccg ccataacaag ctgagtcagt
      121 attaactggc agcgttcgcg tgaaaggtaa gtattctgcg caatttcccc gacggtcgcc
      181 ggttcggtga cgcttaattc attaaacacc actctggcgg tttcggtcat atcctgctgt
      241 tttagcatgc ttttttccct tttccggtta acgtgacata ccaatacctc ttgtcgaaaa
      301 agccagcaag ctgaaagacc agtattcaca actaccagcg cgtttaatgt tctgtgccgt
      361 ttttcagaca taaaaaaacc cgcataaagc gggttctttc aggtgtccat gtctgctatt
      421 cgcctcgcgg tatagctttg cgaagcgtag ctggattgaa acagtttatg cgtaaaaaat
      481 caagacattt tttgagcaaa cgattctcgc atagggatgt atagcgcata ttcagcaaca
      541 gccaaccaat tagcaattcg cttttcgcat gtactaaaac accattccgg gtgcacctca
      601 ttcaacaatt cagccatttt gcgtttactc atcccccgcc cttcgtacct ttgccgcagg
      661 atatcaatca atccaggata acgtgcaagc gctttactta tcaccccatc aatgcgtaac
      721 gcctctgcat cagtacagtg agacaaccag ctcttctgtc tgccagcgat catctctcgc
      781 aagaatgctt ccagctctgg tttatcaatc cctgactccc tgattctacg cagggcttca
      841 ttgattgcgg tttttgtcag ttttttggat gccagcaact gattgaacat atttcctggt
      901 ttgccaccac ctatatacga ccaacgcccc cacatccgta atttcccctg gatccagacg
      961 gcttccagcg tttttagacg taaatgctcg ccgcttttgc ctgtaatttc cgggtatatc
     1021 atatttacga tcactcactc tcaattttgt aaatcttcac gcccagccgt cccccaggaa
     1081 cgcgctgacc gcgcacaata ttgatttcat caaactgctc gtcgtctatg agaagtccgg
     1141 catgcgtcag cgcatccagt ggtgctttca ggatattgtc caggtcacga cgacgtttat
     1201 ccggtggctc tgcaataatc tttatcgcca gccttccgga caggtttaat ttcagccgct
     1261 gctggcgaac aataagcgcc acatcacggc gataacgctc accggctttt gatacaaaat
     1321 atgtgctgcc acgacgtcgc cagtaagtgt tcaccgttgg cgggtaaggc aaaacaaatt
     1381 ctat
//
LOCUS       DAZGLJ010000454         1371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000454 DAZGLJ010000000
VERSION     DAZGLJ010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.466"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            171..>1371
                     /gene="bluF"
                     /locus_tag="ACHK49_005166"
     CDS             171..>1371
                     /gene="bluF"
                     /locus_tag="ACHK49_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO9265609.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWP"
BASE COUNT          387 a          292 c          293 g          399 t
ORIGIN      
        1 tgcatcattt aactgacaat ctatacaaga tttttcgcca actagtgttc aactttttcc
       61 attttttatc aatgtaaaga aacggttatg ttttggtatt aaaagaaaga cacaagaaga
      121 tatgtctgtt accgtcttac tctcgcctca cccattaccc tggattgtta atgcttacca
      181 cccttattta tcgtagccat atacgtgacg acgaacctgt caaaaaaatc gaagaaatgg
      241 tttcgatagc aaatcgcagg aacatgcagt ctgacgtaac agggatctta ctgtttaatg
      301 gttctcattt tttccagctt ctggaaggtc cggaagaaca ggttaaaatg atatatcggg
      361 ctatatgcca ggatccacgg cactataata ttgttgagct gctgtgcgat tacgcgcctg
      421 ctcgccgttt tggcaaagcg ggaatggaat tatttgattt gcgcctgcac gagcgagatg
      481 acgttttaca ggccgtattc gacaaaggca catcaaaatt tcagctaact tatgatgaca
      541 gagcgctaca attttttcgt acttttgtcc ttgcaaccga acaatcaacc tatttcgaga
      601 tccctgccga agactcctgg ctttttatcg ctgacggatc tgataaagaa cttgattcct
      661 gtgccctttc accaactata aacgaccact ttgcctttca tcctattgtc gatcccttat
      721 cgcggcggat aatcgctttt gaagccattg tgcaaaaaaa tgaagatagc ccatcagcca
      781 tagcggttgg gcagcgtaaa gacggggaaa tctacacagc ggatctcaaa agtaaggcgc
      841 ttgcattcac gatggcacac gcacttgagc tcggtgataa aatgatttca atcaatctat
      901 tacctatgac cctggttaac gaacctgacg cagtctcttt tttacttaat gaaataaagg
      961 ccaatgctct ggtgcctgaa caaatcatcg ttgaatttac tgaaagtgaa gtcatatctc
     1021 ggtttgatga gtttgccgaa gcgattaaat cgctaaaggc tgctggtatc agtgtagcaa
     1081 ttgatcattt tggcgcaggt tttgctggtt tgttactcct gtcacgcttc cagcctgaca
     1141 gaattaaaat cagtcaggaa ttgattacca atgttcataa aagtgggcca cggcaggcaa
     1201 ttattcaggc gatcataaaa tgctgtacat cacttgaaat tcaagtcagt gctatgggcg
     1261 tggcaacacc agaagagtgg atgtggcttg aatccgcagg aattgagatg tttcagggag
     1321 atctgtttgc taaagctaaa ttgaatggta tcccttccat agcgtggcca g
//
LOCUS       DAZGLJ010000455         1370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000455 DAZGLJ010000000
VERSION     DAZGLJ010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.467"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..436
                     /locus_tag="ACHK49_005167"
     CDS             <1..436
                     /locus_tag="ACHK49_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFO9265610.1"
                     /translation="FSTIGMLKNISIFRGRFNSKEITEHVFHTTKFSGDEKYIKFHCN
                     SVEELKPSKLDVIAKPGDILIARVGRNFHKKILFVESGYSYISDCIFLIRASGGDKKK
                     LFDFLCSQDGQEELSRASSGVAAQHITMDALKKIHLVRIKHD"
     gene            429..>1370
                     /locus_tag="ACHK49_005168"
     CDS             429..>1370
                     /locus_tag="ACHK49_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO9265611.1"
                     /translation="MTDVELDSNFLIEERADYIDEDTIKNNTIRSGEFFDIIHNALIS
                     PGIKLIVGPRGCGKTHLMRYAWVECKNDDNAPLPLYVSFNKYFKLEPLLKNKPNAIDL
                     FHKWVLAKILLSAYEIACDIEDSEYLDLSTILIADKEFLINLIVRLEQGLSPSLEQEN
                     IVQLISVSSVISSLSSLCDWLERKRVIILLDDAAISLTPEYLYEFFDIVRSLKTSKIA
                     LKASVYPGTTEYGPRFHVAHDAETIDAWISVQHPNYSSVMDAIAQARFPGYDGIPDDL
                     KELFKFSAFGIPRSFLMMLRDYQTTLSQTTQQKFNKII"
BASE COUNT          449 a          205 c          263 g          453 t
ORIGIN      
        1 gtttagcaca atagggatgc ttaaaaatat ttcaattttt agaggtaggt ttaattcaaa
       61 ggaaattacg gaacatgttt ttcatacgac aaaatttagt ggtgatgaaa agtacattaa
      121 attccactgc aactctgtag aagaattgaa gccatcaaaa ttagatgtca ttgctaagcc
      181 tggtgatata ttaatagcaa gagttgggcg aaattttcat aaaaaaatat tgtttgttga
      241 gagcggctat tcttatatca gtgactgtat ttttctgata cgagcctctg gtggagataa
      301 gaaaaaacta tttgattttc tttgttctca agatgggcaa gaggaattat ctcgagcgag
      361 tagtggtgta gccgcacaac atattacaat ggatgcatta aaaaaaatac atcttgtaag
      421 gattaaacat gactgatgtg gaattagata gtaattttct tatcgaggaa cgagctgatt
      481 atattgatga ggatacgata aaaaataata ctatcagatc tggagagttt ttcgatatta
      541 tacataatgc tcttatatcc ccaggcatca aacttatagt tggtcctagg ggatgtggta
      601 aaacacattt gatgcgttat gcttgggttg aatgtaaaaa cgatgataac gcaccattac
      661 cactctatgt ttcttttaat aaatatttta agttagagcc gcttttaaaa aataaaccaa
      721 atgcaattga tctctttcat aaatgggtat tagcaaagat actactctct gcttatgaaa
      781 ttgcttgcga tattgaagat tctgaatatc ttgatttgtc aacgatactc atagcggata
      841 aagagttcct aattaattta atcgtaaggc ttgagcaggg gctttcgcca tcattagaac
      901 aagaaaacat tgttcaattg atcagtgttt cttctgtcat atcctcgttg agttcactat
      961 gtgattggct tgaacgtaaa agagtcatta ttttattaga cgatgcagca ataagcttaa
     1021 ctcctgaata tctttacgag ttttttgata ttgtacgtag tctaaaaacc tcaaaaatag
     1081 ctttaaaggc atcggtttat cctggaacaa ccgaatatgg cccaaggttt catgttgctc
     1141 atgatgctga aacaattgat gcttggattt cggtacagca ccctaattat tcctcagtga
     1201 tggatgctat agcgcaggca aggtttcctg ggtatgatgg cattccggat gacttgaaag
     1261 agttatttaa attctcagca tttggtattc cacgttcttt tttgatgatg ttaagagatt
     1321 atcaaacaac tttaagtcag acaactcagc aaaaatttaa taaaataatt
//
LOCUS       DAZGLJ010000456         1357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000456 DAZGLJ010000000
VERSION     DAZGLJ010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.468"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            64..231
                     /locus_tag="ACHK49_005169"
     CDS             64..231
                     /locus_tag="ACHK49_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO9265612.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            221..823
                     /locus_tag="ACHK49_005170"
     CDS             221..823
                     /locus_tag="ACHK49_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO9265613.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            855..>1357
                     /gene="pabA"
                     /locus_tag="ACHK49_005171"
     CDS             855..>1357
                     /gene="pabA"
                     /locus_tag="ACHK49_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO9265614.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQF"
BASE COUNT          308 a          339 c          398 g          312 t
ORIGIN      
        1 ccggcgtaac ccggatttgc cgcttatact tgtggcaaat ggacacgttc agggaggcat
       61 caagtgaaga aactcaccga taagcaaaag tcccgtctct gggagcttca gcgtaatcgt
      121 aatttccagg ccagtcgccg tcttgaaggc gtcgagatgc ctttagtcac tcttactgcc
      181 gcagaggctt tagcgcgcct tgaagagctg aggaggcact atgagcgata aattcggcga
      241 agggcgcgat ccgtatcttt atcctggcct tgatatcatg cgtaaccggc tgaacatcca
      301 ccagcagcag cggctggaac aggccgctta cgaaatgacg gcgctgcgtg ctgcgaccat
      361 tgagcttggt ccgctggtgc gcggtttacc gcatttgcgc actatccatc gccagctgta
      421 tcaggatatt ttcgactggg cggggcaact gcgtgaagtt gatatttatc agggcgatac
      481 gccgttctgc cactttgctt atatcgaaaa agagggcaat gccctgatgc aggatctgga
      541 ggaagaaggt tatctggttg gcctggagaa agcgaagttc gtcgagcggc tggcgcatta
      601 ctattgtgaa atcaacgtgc tgcatccctt ccgggtggga agtggtctgg cacagcggat
      661 cttcttcgag caactggcga ttcatgccgg gtatcaactg agctggcagg gtatcgaaaa
      721 agaggcctgg aatcaggcaa atcagagtgg ggcaatgggg gatctcaccg cgctgcagat
      781 gatatttagc aaagtggtaa gcgaagccgg ggaatctgag taaaatagcg cggttctttc
      841 gtaccggagc cgccatgatc ctgcttatag ataactacga ttcttttacc tggaacctct
      901 accagtactt ttgtgaactg ggggcggatg tgctggttaa gcgcaacgat gcgttgacgc
      961 tggcggatat cgacgccctt aaaccacaaa aaattgtcat atcacccggc ccctgtacgc
     1021 cagatgaagc cgggatctcc cttgacgtta ttcgccacta tgccgggcgc ttgccgattc
     1081 ttggcgtctg cctcggtcat caggcaatgg cgcaggcatt tggcggcaaa gttgtgcgcg
     1141 ccgcaaaggt catgcacggc aaaacctcgc cgattacaca taacggtgag ggcgtatttc
     1201 gggggctggc aaatccactt accgtgacac gctaccattc gctggtggtg gaacctggct
     1261 cattaccagc atgctttgac gtgacggcct ggagcgaaac ccgcgagatt atggggattc
     1321 gccatcgcca gtgggatctg gaaggtgtgc agttcca
//
LOCUS       DAZGLJ010000457         1341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000457 DAZGLJ010000000
VERSION     DAZGLJ010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.470"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..198
                     /locus_tag="ACHK49_005172"
     CDS             <1..198
                     /locus_tag="ACHK49_005172"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265615.1"
                     /translation="FNNHDGNVIIVNTSSRFELRKKYVGTLKINSGVSTVDPHWPPVS
                     KRIKLIFFSPTLHSFSCIGVS"
     gene            234..>1341
                     /locus_tag="ACHK49_005173"
     CDS             234..>1341
                     /locus_tag="ACHK49_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265616.1"
                     /translation="MSRRKVGNMGRGDFDRLCNASGLIYNSSSEDDSGGWDAIVEFPL
                     LGGIALLNSLNEQPIQCLVQIKSSDGEKKGVQVKLSNMKRFCDTPLPCFFFFARYNKN
                     ANIESAYLVHINSDIMFQVLKRIRQNDIGKKSPLHKLSFTVNYSDEDEIDLTNEYALK
                     NAIEKHIPHGMKKYSEDKLRDLGNVGYNESRYAFNFKVDNNEDYKSLISATLGYPTRI
                     NVKDVRGWDTRFDINFQMEELTAEQAIIEIRSVEAFSTGSIIFEDEKEDVSFKCEFFV
                     SPIALNAPRDLASFRICSDFFDLLVGIKNNKLKIDFESYDKPFHLADIRELLLLMKIL
                     NAPSGDLKLTIKNNEGNETSFLASDNSFSANPITN"
BASE COUNT          438 a          217 c          270 g          416 t
ORIGIN      
        1 tttaataatc atgatggtaa tgtgattatt gtgaatacga gctcaaggtt tgagctccgg
       61 aaaaaatacg ttgggacgtt aaaaatcaac tcgggagtat ccacagttga tcctcattgg
      121 ccccctgtaa gcaagcgcat caagctaata tttttcagcc cgacacttca tagtttttcc
      181 tgcattgggg taagctaaac ctagtatcgt acatatgaaa gagggatgag tttatgagtc
      241 gaagaaaagt tggtaatatg ggacgtggtg attttgacag gctttgcaat gcatctggct
      301 tgatttacaa cagctcctct gaggatgatt ccggagggtg ggatgccatt gtcgaatttc
      361 ctctgttagg tggtatagcc ttactgaata gtttgaatga acagcctata caatgtttag
      421 tgcagataaa gtcttctgac ggagaaaaga aaggtgtaca ggtcaaactt tctaacatga
      481 aacgtttctg tgacacacct ctaccctgtt ttttcttttt tgcgcgttat aataagaatg
      541 ctaatatcga atcagcttac cttgtccaca ttaatagtga tatcatgttt caggttctga
      601 agcggattcg tcaaaatgat ataggaaaaa aatcaccact tcacaaactt tctttcacgg
      661 taaattactc tgatgaggat gagatagacc tgactaatga atatgcactc aaaaatgcta
      721 ttgaaaaaca tattcctcat ggaatgaaaa aatatagtga agataaattg agagatttag
      781 gtaatgtagg atataacgaa agccgctatg cattcaattt caaagttgat aataatgagg
      841 attataagtc tcttattagt gccacgcttg gatatccgac cagaataaat gtaaaagatg
      901 ttcgcggttg ggatactcga tttgatatta attttcagat ggaagaactg acagctgaac
      961 aagcaataat cgaaataaga tctgttgaag cattctcaac aggctctata atattcgaag
     1021 atgaaaaaga agatgtctct tttaagtgtg aatttttcgt ttctcctatt gcacttaatg
     1081 ctccaaggga tctggcaagc tttagaattt gctcggactt tttcgattta ctggttggta
     1141 ttaaaaataa caaattgaaa atagattttg aatcatatga taagcctttt catttagctg
     1201 acattagaga attactgtta ttaatgaaga tattaaatgc cccaagcgga gatctgaagt
     1261 taacaataaa aaacaatgag ggtaacgaga catccttttt agcctcggat aattcatttt
     1321 ctgcgaatcc aattactaac g
//
LOCUS       DAZGLJ010000458         1335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000458 DAZGLJ010000000
VERSION     DAZGLJ010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.471"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(61..273)
                     /locus_tag="ACHK49_005174"
                     /pseudo
     CDS             complement(61..273)
                     /locus_tag="ACHK49_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            423..1091
                     /locus_tag="ACHK49_005175"
     CDS             423..1091
                     /locus_tag="ACHK49_005175"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO9265617.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(1036..1173)
                     /locus_tag="ACHK49_005176"
     CDS             complement(1036..1173)
                     /locus_tag="ACHK49_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFO9265618.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(1146..>1335)
                     /locus_tag="ACHK49_005177"
     CDS             complement(1146..>1335)
                     /locus_tag="ACHK49_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO9265619.1"
                     /translation="KNSLMQVASEHIAPLQDAVDLEIATEEETSLLEAWKKYRVLLNR
                     VDTSTAPDIEWPTNPVRE"
BASE COUNT          391 a          300 c          258 g          386 t
ORIGIN      
        1 gatattactt taatcaaaag ttgacgtgcc agattcgaag taaatttata aaatatcaaa
       61 tcaatataca ttttgcacct tcgagatagt aatgactgtt actgtaacat gagttacaaa
      121 cagcttcata cccagagcta ctgatgtatc aactcgattc aacaataccc gatatttatt
      181 ccatgcctcc agcaacgatc tttcttcctc cgttgcgatt tccagatcta cagcatcctg
      241 cagtggcgca atatactcac tgaattcctg gatgtagaac tgtgtggtga cggtcttcca
      301 gccattcggc tcctgttgta tcgaagcata ccaggctatt tcaatatcgc tatgctgcgg
      361 cagcatttaa ccccttgtaa ttcatcgcca taattgattt aattcacaaa taaaactata
      421 acatggtgaa attaatgaaa aaaaacacag atgatggggc taaaatttac acaccactta
      481 ccctaaagct ttatgactgg tgggttttgg gagtatcaaa tcggcttgca tggggatgtc
      541 ctacaaagga acaccttctt ccacactttc tggaacattt aggtaacaac catctggata
      601 ttggtgttgg aactgggttt taccttactc acgtacctga gagtagtctg atatctttaa
      661 tggatttgaa cgaagctagc ctgaacgcgg catctacaag ggctggggaa tcaaaaatta
      721 aacataaaat tagccatgat gtttttgaac cttatcccgc ggcgttacat ggtcaatttg
      781 attccatttc catgttttac cttcttcact gcctgcctgg aaatatatct acaaaaagct
      841 gtgtaatacg caatgcggcg caggccttaa ctgacgatgg aactctatac ggagccacaa
      901 ttcttggcga tggagttgtg cacaatagct tcggtcaaaa actgatgcgc atttacaatc
      961 agaaaggcat cttttcaaac acaaaagatt ccgaagaagg cttaacacat atactctcag
     1021 agcatttcga gaatgttaaa accaaggttc aaggtactgt agtaatgttt tccgcttcag
     1081 ggaaaaaata gcatccaacc gcagcacgtt cttgcttaag acgtgctgcg gcataatccc
     1141 aatgattact ccctgacagg gttcgtaggc cactcaatat caggtgcagt tgatgtatca
     1201 acacggttca gcaacacccg atactttttc caggcttcca gcaatgaggt ttcttcctcc
     1261 gttgcgattt ccagatctac agcatcctga agtggcgcaa tatgctcact ggctacctgc
     1321 atcaggctgt ttttt
//
LOCUS       DAZGLJ010000459         1303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000459 DAZGLJ010000000
VERSION     DAZGLJ010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.472"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            5..1303
                     /gene="nleA"
                     /locus_tag="ACHK49_005178"
                     /pseudo
     CDS             5..1303
                     /gene="nleA"
                     /locus_tag="ACHK49_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
BASE COUNT          412 a          276 c          275 g          340 t
ORIGIN      
        1 tattatgaac attcaaccga ccatacaatc tggaatcacc tcacaaaaca atcaacatca
       61 tcaaacagaa caaataccct ctacacaaat accgcaatcc gaattacctc taggatgcca
      121 agctggattt gttgttaata ttccagatga tatacagcaa catgcaccgg aatgcggtga
      181 aacaacagct ctactgagct tgataaaaga taaaggtctg ctctcagggc tagacgaata
      241 tatagctcct caccttgaag aaggatccat aggaaaaaaa cattggatat gtttggttta
      301 ttcaatgtta cccaaatggc attagagata cctagttccg tttcaggcat ctctggtaaa
      361 tatggtgtcc agctaaacat tgtaaaacca gatattcatc ctacatcagg taattatttt
      421 ttacagatat tccctctgca tgatgaaata ggttttaatt ttaaagacct tcctggcccg
      481 ttaaaaaatg cattaagcaa cagtaatata tcaaccactg cagtgtcgac tattgcatcg
      541 actggaacat cagccactac ttcgacggta accaccgagc caaaaggccc aataccatgg
      601 tttggattaa cagctcaagt ggttcgtaat catggtgtag aacttcctat agtcaaaact
      661 gaaaatggat ggaagcttgt tggagaaaca ccacttactc ctgatgggcc gaaagcaaat
      721 tacacggagg agtgggttat cagaccggga gaagcagatt ttaaatatgg tgcatctcca
      781 ttacaggcaa ctctagggct ggagtttggc gcacatttca agtgggattt agataaccct
      841 aatactaaat atgccgttct taccaatgct gccgcaaatg cgcttggtgc tttaggggga
      901 tttgcagtat ccagatttgc tagtacagat ccaatgttaa gtcctcatat cggtgcaatg
      961 gttgggcaag cagcagggca tgccatacag tataataccc ctggattaaa gccagacact
     1021 attttatggt gggctggtgc gacactgggg gctgccgatt taaacaaggc cgagtttgaa
     1081 gtagctagat tcactgacta tcctcgtata tggtggcacg caagagaagg agctattttc
     1141 cccaataaag cagatattga acatgccaca ggtgctgata tacgcgcaat ggaagaaggt
     1201 atccctgttg gacagcggca tccaaatcca gaggatgtgg taatcgatat cgaaagcaat
     1261 ggcttaccac atcataatcc atcaaatcat gttgatatct ttg
//
LOCUS       DAZGLJ010000460         1292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000460 DAZGLJ010000000
VERSION     DAZGLJ010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.473"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            26..673
                     /gene="nleG"
                     /locus_tag="ACHK49_005179"
     CDS             26..673
                     /gene="nleG"
                     /locus_tag="ACHK49_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase NleG"
                     /protein_id="HFO9265620.1"
                     /translation="MPVILNFSSERVLSESELEALRHVGRVSQSEQLVVRGRTMRLHH
                     ISFMDSFSVEPVSGGLLDRLSARGHRLLAENLEIQLNRGHTFLQAFRLYMEQSRATPC
                     TRQNVSSAIQNKINSHAFTVSHQDFSCHEQHLNCPITLCIPETGVFVRNAKNSEICSL
                     YDHNALTELIRRNAPHPLSREPFVPEMIVSKDECHFNLIEQYFCILATQNICTRI"
     gene            complement(810..956)
                     /locus_tag="ACHK49_005180"
     CDS             complement(810..956)
                     /locus_tag="ACHK49_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265621.1"
                     /translation="MIFIASSSREQTTASDGKMGTTGYSGNLAEEAYGTDCNVKYKTL
                     KIPF"
BASE COUNT          397 a          269 c          248 g          378 t
ORIGIN      
        1 aacatagact aaagagggag aaaatatgcc tgtcatatta aacttttcga gtgaaagagt
       61 attgtctgaa agtgaactgg aggctttacg tcatgtcgga agagttagtc agagtgaaca
      121 gcttgttgtg agaggccgca ctatgagact tcatcatatt tcatttatgg acagttttag
      181 cgtggaaccg gtttccggcg gactgttgga tcgccttagt gcaagagggc atcgccttct
      241 cgcagaaaat ctggagatac agcttaacag aggccatact tttttgcagg cattccgttt
      301 atatatggaa cagagccgtg caaccccatg tacgcgccaa aacgtcagta gtgcaataca
      361 aaacaaaatt aactctcatg catttactgt tagtcatcaa gacttttcct gtcatgaaca
      421 acacctgaat tgcccgataa cattatgcat tcctgaaact ggtgtttttg tcagaaatgc
      481 aaaaaactca gaaatatgtt cattatatga ccacaatgca ctcactgaac ttatccgacg
      541 caacgctcca caccctctca gccgtgaacc ctttgttccg gaaatgattg tcagcaaaga
      601 tgaatgccat tttaatctaa tagaacaata tttttgcata ttagccacac aaaatatatg
      661 cactagaatt tgactgtatg gttcgattta aatgttgcaa aattaatttt actagttatt
      721 gccatctatt tcatactccc taattcttga tacaatctgt ctgtcaaaat agtagattag
      781 atcgaactta tattacagct atttagtttc taaaatggaa tttttaacgt tttatacttc
      841 acattacaat ctgtaccata agcctcctca gcgaggttgc ctgaataccc tgtcgtgccc
      901 atttttccat cggacgcagt tgtctgctct ctcgaactgc tggcaataaa aatcaaccag
      961 acagcttgat gtccgttaca tgcaggacta atcacgattg gttaccgttt acaaaacttg
     1021 cgtaacattg gaggagaacg aataatattc tctagctggt acgcgttcca tagcgaaacg
     1081 ggcaaggtcc aacagcaaga gctgaaactc agggggattt atcagtcgat ttattcgcca
     1141 tggatgaatc acgctgtacg gttatggtag ctcttagcga caaggcataa tcatcagtac
     1201 accaatgtaa caaccgttga aaaagactca ttattttatg aaccagccat cccatttcca
     1261 gagtggtaaa tatgatttgt taaagtgtaa ca
//
LOCUS       DAZGLJ010000461         1288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000461 DAZGLJ010000000
VERSION     DAZGLJ010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.474"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..735
                     /locus_tag="ACHK49_005181"
     CDS             1..735
                     /locus_tag="ACHK49_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9265622.1"
                     /translation="MAPVKGAGTTLWVYTGKGDAYANPLSDDDWQRLAKVKDLTPGEM
                     TAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFESGDVRAY
                     KIRFPNGTVDVFRGWISSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITPATAIKV
                     TPTSGTVEKGKTTTLTVSFEPESATDKTFRAVSADPSTGTIAVKDMVITVTGVKAGKV
                     SIPVISGNGQFATVAEVTVTEAGAAG"
     gene            749..1180
                     /locus_tag="ACHK49_005182"
     CDS             749..1180
                     /locus_tag="ACHK49_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO9265623.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTGSPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGPVHVDTDITEVAKNNALTDDDFSAGKSSTAS"
     gene            1195..>1288
                     /locus_tag="ACHK49_005183"
     CDS             1195..>1288
                     /locus_tag="ACHK49_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO9265624.1"
                     /translation="MAREMGRPDWRAMLAGMTSTEYADWRHFYRM"
BASE COUNT          320 a          295 c          402 g          271 t
ORIGIN      
        1 ctggcgccgg taaaaggtgc cggtaccact ctgtgggttt acaccggcaa gggtgatgct
       61 tatgcaaacc cgttgtcaga tgatgactgg cagcgactgg ctaaggtgaa ggatctgacg
      121 ccgggcgaga tgacggcaga atcctacgat gataactacc tggatgatga agacgcggac
      181 tggagcgcga ccgggcaggg acagaaatct gcaggtgata ccagttttac actggcctgg
      241 aaaccgggag aagaaggtca gaaagggctt ataggctggt ttgaaagcgg ggatgtgcgg
      301 gcctataaaa tccgttttcc gaatggcacg gtggatgtgt ttcgtggctg gatcagcagt
      361 atcggtaagg cagtgacggc gaaagaagtg atcacccgaa cagtgaaagt gaccaacgtg
      421 ggcaaacctt ccgtggcgga agaacgcagc gaaattacgc cggccactgc aattaaggtg
      481 acaccgacat ccggtaccgt ggaaaaagga aaaacaacca ccctgactgt ttcttttgag
      541 ccggaaagtg caaccgacaa gacgttcaga gcggtttccg ccgatccgtc aacgggaacc
      601 attgctgtga aagatatggt gatcactgtg acgggggtta aggctggaaa agtgagtatc
      661 cccgtgattt ccggtaatgg tcagtttgcc acggtagctg aagtcaccgt tactgaagcg
      721 ggcgctgcag ggtaaacgga ggtcatacat gtttctgaaa acagaacaat ttgaatataa
      781 cggtgtgtct gtcacgcttt ccgaattgtc tgcgctgcag cgtattgagc atcttgccct
      841 cctgaaacgg cgtgcagaac aggcagaatc cagcggcaac ctgcaggtaa gcgtggaaga
      901 tctcgtcaga accggcgcgt ttctggtggc gatgtccctg tggcataacc atccgcagaa
      961 aacggggtca ccgtcaatga atgaggccgt gatgcagatt gagcaggaag tcctgaccac
     1021 ctggcctgct gatgccattg cccgggcgga agatgtggtg ttgcgtctgt ccgggatgag
     1081 cgggcctgtt catgtggata cggatattac cgaagtggcg aaaaataacg cgctgactga
     1141 tgatgatttt tctgcgggaa agtcttcgac ggcgagctga attttgccct cagactggcg
     1201 cgtgagatgg ggaggcctga ctggcgcgcc atgcttgccg ggatgacatc caccgaatat
     1261 gccgactggc gacattttta ccgcatgc
//
LOCUS       DAZGLJ010000462         1271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000462 DAZGLJ010000000
VERSION     DAZGLJ010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.475"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..852
                     /gene="ldtC"
                     /locus_tag="ACHK49_005184"
     CDS             <1..852
                     /gene="ldtC"
                     /locus_tag="ACHK49_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO9265625.1"
                     /translation="SRLVGENKFHVVENDGSSLEAIAKKYNVGFLALLQANPGVDPYV
                     PRAGSVLTIPLQTLLPDAPREGIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLT
                     PTMVTTVSDKRANPTWTPTANIRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGV
                     YLLHGTNADFGIGMRVSSGCIRLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGAR
                     LVEVHQPLSEKIDDDPQLLPITLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRH
                     QVSPQTL"
     gene            complement(935..1192)
                     /gene="bhsA"
                     /locus_tag="ACHK49_005185"
                     /gene_synonym="ycfR"
     CDS             complement(935..1192)
                     /gene="bhsA"
                     /locus_tag="ACHK49_005185"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO9265626.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT          328 a          310 c          322 g          311 t
ORIGIN      
        1 agtcgtctgg ttggcgaaaa caaatttcat gtggtggaaa atgacggtag ttctctggaa
       61 gccatcgcca aaaaatataa cgtcggcttt ctcgctctgt tacaggctaa ccctggcgtt
      121 gatccttacg taccgcgcgc gggtagcgtg ttaacgatcc cgttgcaaac cctacttcca
      181 gatgcgccgc gcgaaggcat tgtgatcaat attgcggagc tgcgtctcta ttactacccg
      241 ccgggtaaaa attcggtaac cgtgtatcca ataggtattg gtcagttagg tggtgacacg
      301 ctgacaccga caatggtgac caccgtttca gacaaacgtg caaacccaac ctggacgcca
      361 acggcaaaca tccgcgcccg ttataaagca cagggaattg agttgcctgc ggtagtgccg
      421 gctggaccgg ataacccaat gggccatcat gcgattcgtc tggcggccta tggcggcgtt
      481 tatttgcttc acggtacgaa cgccgatttc ggcattggca tgcgggtaag ttctggctgt
      541 attcgtctgc gggatgacga tatcaaaaca ctctttagcc aggtcacccc aggcaccaaa
      601 gtgaatatca tcaacactcc gataaaagtc tctgccgaac caaacggtgc gcgtctggtt
      661 gaagtacatc agccgctgtc agagaagatt gatgacgatc cgcagctgct gccaattacg
      721 ctgaatagcg caatgcaatc atttaaagat gcagcacaaa ctgacgctga agtgatgcaa
      781 catgtgatgg atgtccgttc cgggatgccg gtggatgtcc gccgtcatca agtgagccca
      841 caaactctgt aaatcctgga taaaaaaaat ccccgcgaca cggcatgtcg cggggaaaaa
      901 atcagcagta gcaggcatta atgagggtta atgcttattt ataaattact gctgttccat
      961 ggagggtatt cggaccggtt acagaagtaa tacggaaaga ttttgcgccc atctcatccg
     1021 ctttttgcgc cagctgctct tccagcgatc ccagatttgt ccccgcgtta gcactgattg
     1081 taccgacttt ttgttggcct tctggcgttg actgaacttc gacagccgca aagctggcaa
     1141 atgacatgga gcttaaaatc gccgcagcga tgagggtttt tacgtttttc ataatagtgg
     1201 ccttatgcag atgaatgacg tcgcaagcaa ttacttaacg atcgataact aaatgataga
     1261 tgtgatctgg a
//
LOCUS       DAZGLJ010000463         1267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000463 DAZGLJ010000000
VERSION     DAZGLJ010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.476"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(63..941)
                     /gene="glxR"
                     /locus_tag="ACHK49_005186"
     CDS             complement(63..941)
                     /gene="glxR"
                     /locus_tag="ACHK49_005186"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO9265627.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(1041..>1267)
                     /locus_tag="ACHK49_005187"
     CDS             complement(1041..>1267)
                     /locus_tag="ACHK49_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO9265628.1"
                     /translation="MEGELTNTMTQWADKIGHLQIADNPHRGEPGTGEINYDYLFKVI
                     ENSDYNGWVGCEYTPQTTTEAGLRWMDPYR"
BASE COUNT          314 a          339 c          275 g          339 t
ORIGIN      
        1 ctttcttttt taatattgct ggccgattca ttttctgata tcggccattt ttattgcggg
       61 tatcaggcca gtttatggtt agccattaat tccagcgcct gcactaacgc agagtgatcc
      121 aactggctgc caccgtttgc cgcgcaggta ttaaataact cctggcaggt cgcagtgttt
      181 ggcaggttca gcgctagcgc tttcgcactt tgcagtgcca ggttgagatc tttctggtgc
      241 agcgcaattt tgaaacctgg attaaaagtg cgtttaatca tgcgctcacc gtgaacttcc
      301 agaatacgtg aggaagcaaa gccgcccatc agcgcctggc gcacacgtac cgggtccgca
      361 ccggcttttg aagcaaatag cagggcttca gaaaccgctt caatattgag cgccacaata
      421 atctgatttg ccactttgca ggtttgacca tcgccgttac cgcccacgag ggtgatattt
      481 ttaccgagca gttcaaacag cggtttaaca cgttcaaata ccgcttcatc accgccaacc
      541 ataatcgaca acgtcccttc acgcgcaccg atttcaccgc cggagactgg cgcatcgaga
      601 taatcgccgc ccagttcatt cacctgacga gcgaaacgct tagtttcaat cggggaaatg
      661 gagctcatat caacaatggt tttgcccttc agcgaggctt tggtacaacc attttcaccg
      721 aacagaactt cttcaacctg tggtgtatct ggcaccataa taaaaatgat gtccgatgct
      781 tccgttacct ggcgcgcagt ttcaacactg acggcaccca gtgacagtaa ttcatcagca
      841 accggaccaa tggttgtgac atgcagttga tgaccggcac gcgccagatt aatggccatc
      901 ggtgtaccca taatgcctaa gccaataaat cccagtttca taattaacct cttttaaatt
      961 tcgcttttcc tgaattcaga caacacgatc ccctgccaac ttagcggcct aaaaaagcat
     1021 tgcctgaaca gcgttacgtt ttaacggtac ggatccatcc agcgtaaacc ggcttccgtg
     1081 gtggtttggg gtgtatattc acacccaacc caaccgttgt agtcagaatt ttcgattacc
     1141 ttaaagagat aatcataatt aatttctccg gtccccggtt cgccgcgatg cggattatca
     1201 gcaatttgca ggtgaccaat tttatcggcc cactgagtca tggtgttggt taattcacct
     1261 tccatcc
//
LOCUS       DAZGLJ010000464         1257 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000464 DAZGLJ010000000
VERSION     DAZGLJ010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.477"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(59..403)
                     /locus_tag="ACHK49_005188"
     CDS             complement(59..403)
                     /locus_tag="ACHK49_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265629.1"
                     /translation="MSDLSMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEKIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(432..599)
                     /locus_tag="ACHK49_005189"
     CDS             complement(432..599)
                     /locus_tag="ACHK49_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265630.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(672..956)
                     /locus_tag="ACHK49_005190"
     CDS             complement(672..956)
                     /locus_tag="ACHK49_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO9265631.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEIKTITHPHFGDKPVKVFAIKVNIGNE"
     gene            complement(949..1092)
                     /locus_tag="ACHK49_005191"
     CDS             complement(949..1092)
                     /locus_tag="ACHK49_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265632.1"
                     /translation="MKVWVYDPETILNVEERRRTEVYIHNLNEDGFAPVLVKATPQEV
                     NRG"
     gene            complement(1102..1251)
                     /locus_tag="ACHK49_005192"
                     /pseudo
     CDS             complement(1102..1251)
                     /locus_tag="ACHK49_005192"
                     /inference="COORDINATES: protein motif:HMM:TIGR03655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
BASE COUNT          308 a          300 c          260 g          389 t
ORIGIN      
        1 atggtaggtc tggatatcat tgagccatga acaggcctca tcgagtgtga ggctgtggtt
       61 agtcctttcg taactcgctg attctcctgt aagtctctgg tgctttgttt ccgtgtatct
      121 tcatttcaga cttcaacaga gcaacgaggg aatcccattc gttgaggatg cctttgaatg
      181 ccggaacgcg ctttgcaacc ttgtcgaatg aatctctgat ttctggaatc ttctcaacaa
      241 gtgcaacgca tcgtcggaag tctgctgcgt catggggagc gccgaagtga tgaccataga
      301 tattcttttt caggccacat gcgattgagg caagagttgc gctactgatg ccaacatcgc
      361 cagtcgattg ccatttcaaa accttcatag acaaatctga catttcttgt ctccaataaa
      421 aaaaacctcc atcaggcggc ttggtgttct ttcagttctt caattcgaat attggttacg
      481 tctgcatgtg ctatctgcgc ccatatcatc cagtggtcat agcagtcatt gatgttctct
      541 gcttcgataa ctctgttgaa tggttctcca ttccattcac ctgtgactcg gaagtgcatt
      601 tatcatctcc ataaaacaaa atccgccgta gcgagttcag ataaaagaaa tccccgcgag
      661 tgcgaggatt gttattcatt gccgatattc acctttatcg cgaacacctt taccggttta
      721 tcaccgaagt gcggatgtgt gattgtcttg atttcatatc cgtcatacgg aacatcaatt
      781 ctgcggctgg aatcgtcgcg cttcggatat ccctttgtga taatcaggcg gtcatactcc
      841 cggaacataa ttcgcttatt ccaatagtca ttacacaggc gatactcttc cgttttctct
      901 cctcgaatca tggcatcgaa gtattcacct ttgacggcaa gttgtaggtt agccacggtt
      961 aacctcctgc ggagtagcct ttacaagcac tggtgcaaat ccatcttcat taaggttatg
     1021 aatatagact tctgttctcc tgcgctcttc aacgtttaat attgtttctg gatcataaac
     1081 ccatactttc attcgactat tccatgaaga aatcgcttca gatctagttc gtttttctgg
     1141 tccttgggca ccacatttgc atgacacata gcgcattttc cctctgatac tgaatgagta
     1201 tccgatgtta agcacagtgg aaccacagaa tgggcagcga tatagattca taaatca
//
LOCUS       DAZGLJ010000465         1252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000465 DAZGLJ010000000
VERSION     DAZGLJ010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.478"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>1252)
                     /locus_tag="ACHK49_005193"
     CDS             complement(<1..>1252)
                     /locus_tag="ACHK49_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFO9265633.1"
                     /translation="PTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGRDRITPRTV
                     SAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEKASDLCLEL
                     IDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESLLTSLTQAA
                     QCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYCLWLYEDYS
                     AGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALDIFLSQLKY
                     YLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGLADDAEWLF
                     YNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEAAVCCVPNT
                     ARTIPAFVENMFQENNNGFTALFYG"
BASE COUNT          369 a          264 c          229 g          390 t
ORIGIN      
        1 gccataaaat aatgcagtaa aaccattgtt attttcttga aacatatttt cgacaaaagc
       61 cggtatggtt cttgctgtat tcggtacgca gcagacagca gcctcctcat gagtagggga
      121 gtatttatat ctggtattaa actgattact gtccggatgt ttcctttcat ttgcttcata
      181 acaattatca gtcttacagt acattatcgc gtgaagtatg ggatgctgca tgccaaaact
      241 agcattatag aatagccact cagcatcatc ggccagacca atattagtgg tcagttttat
      301 taacataaca taagtgctaa atagttcatg aacagaacaa tattcatagc ctgtataagt
      361 ggcattagct gtgcgtcctg taatccactc atcaccaatg ggagcgccag atggtgtaag
      421 ataatatttc aactgggata aaaaaatatc taatgcagtt tgatactgcg gccgttggct
      481 cgctgcgatg gctaatgccc taatatgctc atacgtatgt acgccatgac ctttaagaag
      541 gtaaccaggg tctatgacat ttttatattg cagatcttgc tccgacacgc atcctgcgga
      601 ataatcttca tacagccaca agcaatatga gagatattca atgttattgg ttatttcata
      661 aaggcggtag aaaacatctg ttattgttaa tccgtggcta tgtccagcaa aaccctgttc
      721 cacaacgaaa ggatgtgacg tttctttagg gtagccagcc ataatgcatt gtgcggcttg
      781 ggtaagagaa gttaataaac tctcattacg ggtggactca taacatccaa gcacaaaacg
      841 caaggcagta gcttgtgccc agaactctcc attttctgct ttacagttaa attttaatga
      901 tgaatcataa attccaatat agccatcatc atcttgcgta tcgataagtt ccaaacataa
      961 atcgctcgct ttttcctgcc attctttatt atctaacaag taggcactgc ggcaatatcc
     1021 atccttccaa ttcgattgag tttcactatc ccaccaataa tattgcatca tgttatcatc
     1081 gggattattc tgttttttaa taacgccatc ttcagcagat acagtgcgtg gagttatacg
     1141 atctcttcca tatattttat gctccgtaat aagatgggga actaatttat ccagttctcc
     1201 tatatgaccg ttcatatacc attccatctt attctttaac caaccagtgg gc
//
LOCUS       DAZGLJ010000466         1246 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000466 DAZGLJ010000000
VERSION     DAZGLJ010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.479"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(42..383)
                     /locus_tag="ACHK49_005194"
     CDS             complement(42..383)
                     /locus_tag="ACHK49_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO9265634.1"
                     /translation="MRDMYEVLDRWGAWAAADNSGVDWQPVAAGFKGLLPHGKKTRQQ
                     CDDDEGIMIDSCVARLRKYKPEEYELVIAHFVIGISLRTIAKKQKCSDGTIRKELQTA
                     LGFLDGVLSML"
     gene            complement(401..>1246)
                     /locus_tag="ACHK49_005195"
     CDS             complement(401..>1246)
                     /locus_tag="ACHK49_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9265635.1"
                     /translation="AVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGC
                     QWPHSDWHSENMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRR
                     DLGFDDSHVVTMPELCWWLIRNDLADALPESAARKVLRLPKPVVPSVTRESDLVPSVP
                     ATSIIQDKAKKVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDP
                     HHLIGHGQGGMATKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALA
                     IGVLA"
BASE COUNT          317 a          368 c          274 g          287 t
ORIGIN      
        1 taaagcaaca gagaaaaaaa ggggggtaat tccccctaat attacaacat tgaaagcaca
       61 ccatccagaa aaccaagagc tgtttgcaac tcttttctta ttgtcccatc agaacacttc
      121 tgcttctttg caatagttct tagtgagatg ccgataacaa aatgagcaat aaccaactca
      181 tactcttcgg gcttatattt ccgcaatcgc gcaacacagc tgtcaatcat gattccttca
      241 tcatcatcgc attgttggcg tgttttcttt ccatgaggta gtaaaccttt aaaccctgca
      301 gcaacaggct gccagtccac accactgtta tctgctgcag cccatgcacc ccagcggtcc
      361 aatacttcat acatatcacg catcaacttt ctccacaaaa tcaggccagc acgccaattg
      421 ccagtgcacg atcgataaaa cgaaatatca gctccagctg ggagccatac ttctcttcaa
      481 atgccacggt atccgcatgc agctcgtcgt gatgctttct gcacaaaggc aacacaaaga
      541 ggtcatgcgc ttttgtagcc attccaccct gaccgtgacc tatcaggtgg tggggatcat
      601 cagcaggttt tccacaacat gcgcacggct gcgtcttaac ccatcgcgtg tacttttcat
      661 taacccagcg gcgacgtttt gggcgtaaca taaaagactc cggcgactcc ggatccactt
      721 tcagcgccag cacctttttc gctttatcct ggatgatgct ggtggcagga accgaaggca
      781 caaggtcact ttcccgggtg acagacggca caacaggctt cggtaatctc agtaccttac
      841 gggctgcact ttccggtaag gcatccgcca gatcattacg aatcagccac cagcacagtt
      901 ccggcattgt cacaacgtga ctgtcatcaa aaccgagatc ccgacgcacg acagacaaca
      961 cccagcgggc acagttatcc gttgccattg attccagccg ttccgtgaac tgatcgcgca
     1021 gctggttatc gcagtgccag cacagacgga ttgcgcccgg cgcgtgtcgc attgtggtca
     1081 tgttctcgct gtgccagtcg gaatgaggcc actggcagcc tttttcacga agtaaccagc
     1141 tttcaagaca ttccacgcca ccagcacgac ggatcactgc ctcattgcgg aacacggccc
     1201 gaacggcagg atcatccgcc agcggttgtg atgccgccgg aacggc
//
LOCUS       DAZGLJ010000467         1242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000467 DAZGLJ010000000
VERSION     DAZGLJ010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.480"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(278..1153)
                     /locus_tag="ACHK49_005196"
     CDS             complement(278..1153)
                     /locus_tag="ACHK49_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFO9265636.1"
                     /translation="MFSIRTLLPISASVSVPTKQSQSIPITLAGRTIEKAQEKEGLLV
                     FLGMKSVNDYTLNILGQNVSRVTTGKKPYDLLFLNDATKQDFDKRKMEFTYPGANKSH
                     LQSSNSDVVAAAAISITATEMKTILPDDLTPGKYNKIYLSGHGSAGLPLLKCGDEFLS
                     PSDIVDRIVQHNLHEIDDIRLTSCNSANIIKNKDFSPDEIEKSANMNNGWLARALFGQ
                     KRSLAEHVYAEFERRGINVSISGYHGTGVFYVPEHGKPTTHLRSTTVPATPEHTVRRS
                     DYRATLGRTQPIDIE"
BASE COUNT          346 a          248 c          238 g          410 t
ORIGIN      
        1 gtcagcattt cataattatg gatgcagacc aacagaaaca gcattgtaaa taaaatgtaa
       61 taattacata ctattagtga ttctcatgca tcgtaagcgg ctcgccagaa ccgtattgat
      121 atttactgag cgctcagatc aactttccat ggcaacagat cgcgtacccg gtttgccggc
      181 cagtcctgga tatgctcccg ccgacaatat taatggccag gaaatgttta aaacaatgat
      241 tctgtttcct aaccatcaag tgtatatgct ggctcaatta ctcaatatct atgggttgag
      301 ttctacccaa agtggctctg tagtcgcttc ttcttacagt atgttcaggt gttgcaggca
      361 cagttgtgga gcgtagatgc gttgttggtt taccatgctc tggtacataa aaaacgccag
      421 tgccatggta acctgatata gaaacgttaa ttccgcgacg ttcaaactcg gcatagacgt
      481 gttctgctaa agacctcttt tgaccaaata atgccctggc caaccagccg ttattcatat
      541 ttgcggattt ttctatttca tcaggagaga agtctttgtt ttttattatg ttggctgagt
      601 tacaggatgt taatctgata tcatctattt catgaagatt atgttgaaca atgcggtcga
      661 caatatctga cggtgataaa aattcatctc cgcacttaag aagaggtaga ccagcagaac
      721 catgcccaga cagataaatt ttgttgtatt ttcctggtgt taaatcatct ggcaggatgg
      781 ttttcatctc tgtcgctgta atacttatag ctgcagcagc aacaacatcg ctattacttg
      841 attgtagatg gcttttattt gctccaggat atgtaaactc cattttcctt ttatcaaaat
      901 cttgttttgt agcatcattc aggaataata aatcatacgg ttttttcccc gttgtgactc
      961 ttgaaacatt ttggccaaga atattaagag tatagtcatt aacggatttc attcctaaaa
     1021 aaacaagtaa tccttctttc tcttgcgctt tttcgattgt tctccctgct aaagttattg
     1081 ggatggattg agattgtttt gtcggaactg atacgctggc gctaataggt agtagggttc
     1141 ttatgctaaa catacaacct ctttcttaat tttcgaacct gtttaggatt ctgtgtaaat
     1201 tcaaaataaa cctgtctgaa cattcctaac aaacatccac cg
//
LOCUS       DAZGLJ010000468         1240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000468 DAZGLJ010000000
VERSION     DAZGLJ010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.481"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            244..1062
                     /locus_tag="ACHK49_005197"
     CDS             244..1062
                     /locus_tag="ACHK49_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO9265637.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            1153..>1240
                     /locus_tag="ACHK49_005198"
     CDS             1153..>1240
                     /locus_tag="ACHK49_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9265638.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVAT"
BASE COUNT          304 a          329 c          312 g          295 t
ORIGIN      
        1 agatgaattt atttaatttt gggggaagat acaaaaacaa ccagtgaaca tatttccggc
       61 gggttatccg caatgctgaa ggtcagttgc actggcgatg ccggaataca gcttaatacg
      121 tggttcgcca gtgaagggct tctcagacaa taaacctcat tcatttcacc catcaggccg
      181 cgtcttctcc gggagacgcg gctttttcat ttatatcgct taatcattca taaggagcaa
      241 atcatgagat tagccagtcg ttttggttat gcgaaccaga tacgtcgtga tcgtccgctg
      301 acacacgaag aactgatgcg ccatgtaccg agtatttttg gggaaaaccg acatacctct
      361 cgcagtgaac actatgcgta cattcccact atcaccgtcc tggaaaatct gcagcaggaa
      421 ggctttcagc cgttctttgc ctgccagacc cgcgtgcgcg accagagccg ccgggaatac
      481 acaaaacaca tgctgcgtct gcggcgggcc ggacagataa ccggtcagca tgtccctgaa
      541 attattctgc tcaactccca tgacggttca tccagctacc agatgttacc cggatatttt
      601 cgtgccattt gtaccaatgg actggtctgc ggtcagtctc tgggagaatt gcgtgttcca
      661 caccggggaa atgtggtgga cagagtcatt gaaggggctt acgaggtggt gggcgttttt
      721 gaccggatag aggagaagcg tgatgccatg cagtcgctgg tcctgccgcc accggcacgc
      781 caggcgctgg cacaggcggc actgacttac cgttatggtg acgaacatca gcccgtcacc
      841 accgccgaca ttctgacacc acgacgccgg gaggattacg gtaaggacct gtggagtgct
      901 tatcagacca tccaggagaa tatgctgaaa ggcgggattt ccggtcgcag tgcaaaagga
      961 aaacgtatcc atacccgggc cattcacagc attgataccg acattaagct caatcgcgca
     1021 ttgtgggtga tggcagaaac gctgctggag agcatgcgct gatgccgttt cactgaaagt
     1081 gcagtcctga ctcacatctg ttcctgcccc ggggcttctt ccattcattt actttttata
     1141 aggaataacc tcatgacaac cgtttcgcat aattccacca caccttctgt ttccgtaacc
     1201 actgcatcag ggaataaccc acctcagctg gttgccactc
//
LOCUS       DAZGLJ010000469         1238 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000469 DAZGLJ010000000
VERSION     DAZGLJ010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.482"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1238
                     /locus_tag="ACHK49_005199"
     CDS             <1..>1238
                     /locus_tag="ACHK49_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO9265639.1"
                     /translation="VPLAKAWGNAVITLTESNAGFMDRVSAGASLLFPAGPVLMEFAT
                     MVGGRSEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVFINNQPAARKGDKLECSA
                     AIVGGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASYLLPPAGLLGKLKNLARL
                     GKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVTDPVDPVTGAYCDERTDF
                     TLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQGNRVDIISQGATLNFAF
                     DGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAFPGMRLRHPVTDDTSDDR
                     LAHSPDDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDGIRLKLTYHASGYLKAIH
                     RTDNGIQTLATYEQDARGRLT"
BASE COUNT          282 a          350 c          380 g          226 t
ORIGIN      
        1 gtaccactgg cgaaggcctg ggggaacgct gttatcaccc tgacggaaag caatgccggt
       61 tttatggatc gcgtcagcgc cggcgcatcg cttctgtttc ccgccggtcc ggtattaatg
      121 gagtttgcca ccatggtggg cgggcgtagc gaaatcaaaa aagatgtgga tttcccggaa
      181 gccggtgagg acacggcgct ctgcgacaag gagaacaaac caccgaggat agcccagggc
      241 agtagcaacg tctttatcaa caatcagcct gccgcgcgca agggcgacaa actggagtgc
      301 agtgcggcga tcgtgggagg ttcgccggac gtctttattg gcggtgaaca ggtgacctat
      361 ctggatatcc agccggagtt cccgccgtgg cagagaatga tcctgggggg aataacgata
      421 gccagctatc ttctgccgcc tgcgggactg ctgggaaaac tgaagaatct ggcgagactc
      481 ggcaaactgg gaaacctgct ggggaaaagc gggaagctgc tgggcgcaaa gctcggcgcg
      541 ttgctgggga aaacaggtaa gtcgttaaaa agtattgcca ataaagtcat cagatgggta
      601 acagatcctg tcgatccggt aaccggcgca tactgcgacg aacgtaccga cttcaccctg
      661 ggccagaccc tccccctctc cttcacccgt ttccacagtt ctgtactgcc gctgcatggc
      721 ctgaccggcg tgggctggag cgactcctgg agcgaatacg cctgggtgcg tgaacaggga
      781 aatcgggtag atattatcag ccagggagcc acgctgaact tcgccttcga cggtgacagt
      841 gatacggcgg ttaacccgta tcacgcccag tacattctgc gccgccgcga cgattacctg
      901 gagctgttcg acagggatgc actgagcagc cgcttctttt atgacgcctt tccgggaatg
      961 cgtctgcgcc acccggtgac tgacgatacc agcgatgacc gcctggcaca cagccccgac
     1021 gaccggatgt atatgctggg cgggatgagc gacaccgcca gcaaccgcat cacgtttgag
     1081 cgcgacagcc agtaccggat cacgggtgtc agtcacaccg acgggatccg gcttaaactg
     1141 acgtaccacg ccagcggcta cctgaaagcc attcaccgca cggataacgg catacagacg
     1201 ctggcgacct acgaacagga tgcgcggggg cggctgac
//
LOCUS       DAZGLJ010000470         1233 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000470 DAZGLJ010000000
VERSION     DAZGLJ010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.483"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(4..1149)
                     /locus_tag="ACHK49_005200"
     CDS             complement(4..1149)
                     /locus_tag="ACHK49_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EspG domain-containing protein"
                     /protein_id="HFO9265640.1"
                     /translation="MINGLNNNSASLVLDAAIRINSDFKKQWNDMSCAEKLLKVLSFG
                     LWNPTYTRSERQTFQELLTVLEPVSPAPNELGRIYANFADGSSLRISVTNSELVEAEI
                     RTPDNEKILMLLESNEQNRLLQSLPINLHMPYIQVHRALSKMDLTDHKSMHNLLSFTS
                     KLSATLIPHNTQTDPLSGPTPFSSMFMDTFRGLGNAKLSLNGVDIPVDAQKLLRDALG
                     LKDTHSSLARNVINNGISRHHAKQIARESSGSDKQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALMLTHTRISQAREYNVERSLDVPNACINISISQSPDGSIHVASHTGILIMAP
                     EDRPNELGMLTNRTSYEVPQGVKCEIDEMVRTLQPRYGASETYLKNI"
BASE COUNT          341 a          234 c          257 g          401 t
ORIGIN      
        1 tttttaaatg tttttcaggt atgtttcaga tgcgccatac cttggttgta gcgttcttac
       61 catctcatcg atttcacatt tcacaccttg tggcacttca taggaggtcc tgttcgtcaa
      121 cattcctaac tcgttggggc ggtcttctgg cgccattatc agaatcccgg tatggctggc
      181 aacgtgaatg cttccatctg gtgattgaga gatacttatg ttaatgcacg cgttaggtac
      241 atctaacgat ctttccacat tgtattctct ggcttgagag atccttgtat gtgtcaacat
      301 taaggctggc acattgaaag attgatagaa agccgagcat atggctgttg ccgcttcggg
      361 gtggcataaa aactccacaa cttcagcttt ctgtttatcg ctgccgctgc tttcccttgc
      421 tatttgcttt gcatgatgac gagaaatacc attatttata acattccggg caagtgatga
      481 atgtgtgtct ttaagtccta gtgcatcgcg aagcaatttt tgtgcatcta caggtatatc
      541 aacaccattg agtgaaagct ttgcattgcc gagtcctcga aatgtatcca taaacataga
      601 gctgaatggt gtaggcccgg aaagcggatc tgtttgagta ttatgaggaa tcaaggttgc
      661 tgatagtttt gacgtaaaac taagtagatt gtgcattgac ttatgatcag tcaggtccat
      721 cttagataag gcacgatgaa cctgaatgta tggcatgtgg agattgatgg gtaaagattg
      781 taataaccta ttttgttcgt ttgactccag gagcataaga atcttttcat tatctggtgt
      841 gcgaatctcg gcttcaacaa gttcgctgtt agtgacggaa attcttaagg atgagccatc
      901 tgcaaaatta gcatatattc tgcccaattc attaggcgcg ggagatacag gttctaaaac
      961 ggttaacaac tcttgaaatg tttgcctttc actacgagta tacgttggat tccataaacc
     1021 aaaactaaga acttttagta atttttcagc gcatgacata tcattccact gctttttaaa
     1081 atcagaatta attctaattg cagcatctaa aactaaagat gcggaattat tattaagtcc
     1141 gtttatcatg agagattccc ctgtctcatt tattatgaat gattcgacaa cagatatcat
     1201 atagcatgat atagtgtcaa taatatataa gct
//
LOCUS       DAZGLJ010000471         1227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000471 DAZGLJ010000000
VERSION     DAZGLJ010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.484"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            267..1211
                     /gene="leuO"
                     /locus_tag="ACHK49_005201"
     CDS             267..1211
                     /gene="leuO"
                     /locus_tag="ACHK49_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO9265641.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          331 a          247 c          281 g          368 t
ORIGIN      
        1 atacattttg tagatgattg agtattcgcg gtagtcatga ttagattgtt ttcgcaacaa
       61 aaacattatg gattattatg ctgtggtaaa tgactcattc cacggcaatg gattctgttt
      121 ttatcagaac ccgtatcttt atgttttccg aattttactc attttgcttt ttcttatttt
      181 atatgcatga taaatcatat tcttcaggat tatttctctg cattccaata agggaaaggg
      241 agttaagtgt gacagtggag ttaagtatgc cagaggtaca aacagatcat tcagagacgg
      301 cggagttaag caagccacag ctacgcatgg tcgatctcaa cttattaacc gttttcgatg
      361 ccgtgatgca ggagcaaaac attacccgtg ccgctcatgt tctgggtatg tcgcaacctg
      421 cggtcagtaa cgctgttgca cgcctgaagg tgatgtttaa tgacgagctt tttgttcgtt
      481 atggccgtgg tattcaaccg actgctcgcg catttcaact ttttggctca gttcgtcagg
      541 cattgcaact agtacaaaat gaattgcctg gttcaggttt tgaacccgcg agcagtgaac
      601 gtgtatttca tctttgtgtt tgcagcccgt tagacagcat tctgacctcg cagatttata
      661 atcacattga gcagattgcg ccaaatatac atgttatgtt caagtcttca ttaaatcaga
      721 acactgaaca tcagctgcgt tatcaggaaa cggagtttgt gattagttat gaagacttcc
      781 atcgtcctga atttaccagc gtgccattat ttaaagatga aatggtgctg gtagccagca
      841 aaaatcatcc aacaattaag ggcccgttac tgaaacatga tgtttataac gaacaacatg
      901 cggcggtttc gctcgatcgt ttcgcgtcat ttagtcaacc ttggtatgac acggtagata
      961 agcaagccag tatcgcgtat cagggcatgg caatgatgag cgtacttagc gtggtgtcgc
     1021 aaacgcattt ggtcgctatt gcgccgcgtt ggctggctga agagttcgct gaatccttag
     1081 aattacaggt attaccgctg ccgttaaaac aaaatagcag aacctgttat ctctcctggc
     1141 atgaagctgc cgggcgcgat aaaggccatc agtggatgga agagcaatta gtctcaattt
     1201 gcaaacgcta actgaatgca gaatagg
//
LOCUS       DAZGLJ010000472         1226 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000472 DAZGLJ010000000
VERSION     DAZGLJ010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.485"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(45..371)
                     /gene="ybfB"
                     /locus_tag="ACHK49_005202"
     CDS             complement(45..371)
                     /gene="ybfB"
                     /locus_tag="ACHK49_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HFO9265642.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(371..>1226)
                     /locus_tag="ACHK49_005203"
     CDS             complement(371..>1226)
                     /locus_tag="ACHK49_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO9265643.1"
                     /translation="ESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLAL
                     ISTEGATAWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQG
                     RYITQDPIGLKGGWNFYQYPLNPISNIDPLGLETLKCIKPLHSMGGTGERSGPDIWGN
                     PFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEAAGRCDLVETDNSC
                     VENCLKGKFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLETCKMKCSGNNIGRFI
                     RFVFTGVM"
BASE COUNT          345 a          305 c          225 g          351 t
ORIGIN      
        1 tcaatgccca ggttacatat aataataaga gcaattaact taatttattt gtttataatg
       61 cgcttactta atacaaacca ttgaatatac cctcctgaca gaaaataaag ccatattatt
      121 atcgccataa tatacttact atcaatcctt acgccaataa tgtctgatac agtccctata
      181 aatattagag caataaagaa tactatgcct gttggaaaac atattaccat cattccataa
      241 gacatgagac taattaactc gctcacatca cgagtggagt ctaatagtga aagtctatgc
      301 atggaaaaga atagtattaa aagcgataaa gccagccata tagctctgaa taaaaaaata
      361 atatatttca ttacatcact ccggtgaata caaatctaat aaaacgtcca atgttatttc
      421 cggagcactt catcttacat gtttctaaag aatcattaga ccagtcttgg cagtttttaa
      481 atagccctaa atttataggt gtgaatatat caggcaatac agaataacgc ggccttacct
      541 ctttaaactt cccttttaaa cagttctcca cacaactatt atcggtttca acgaggtcac
      601 aacggccagc agcttctttt gtatcattac ttgctttacc tggcccccat agaccatctc
      661 cttttgattc tccccgttgg tcttggccac cacaagtata gtccccttta ccatctggga
      721 cacaaagata ttgatgatag aacggattcc cccatatatc tggaccgctt ctttcaccag
      781 ttccgcccat tgaatgcagt ggcttaatgc attttagtgt ttctaatcct aatggatcta
      841 tatttgagat cggattcaac ggatactgat aaaaattcca tccccccttc agcccaatcg
      901 gatcctgagt gatataccgc ccctgcagcg ggtcataata gcggtggcgg ttgtaataca
      961 ggccggactc ctcatcatac tgctgccccg gcagccggat aagctgctgc agctgatgcg
     1021 ggttctcttc attcagcagg ttgccccatt catcatattc tgcgcaccac gctgttgccc
     1081 cttccgtgct gatgagcgcc agcggcaggc cgcgatggtc gcagtggtac aggtggattt
     1141 ttcgcgccgg cgtgtacacc gggtccatct ggttttgcat ctgctccacg gtcaggccgc
     1201 acgatgccag ccagcggcgg ctttcc
//
LOCUS       DAZGLJ010000473         1222 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000473 DAZGLJ010000000
VERSION     DAZGLJ010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.486"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            44..1200
                     /locus_tag="ACHK49_005204"
     CDS             join(44..293,293..1200)
                     /locus_tag="ACHK49_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HFO9265644.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTVAELESEILQLRKALNEARLERDIFKKSNSVFCTGVAEKYALI
                     EQWRQQFPIEAMCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGT
                     RRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNSNHNLPVAPNLLNQTFA
                     PTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALR
                     SQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWGTLKNE
                     SLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          359 a          286 c          311 g          266 t
ORIGIN      
        1 gacactcctt cctgaaatag aatggcatca gaaggagcta ataatgagca gaaaaaccca
       61 acgttactct aaagagttca aagccgaagc tgtcagaacg gttcttgaaa atcaactttc
      121 gatcagtgaa ggcgcttccc gattatctct tcctgaaggc actttaggac aatgggttac
      181 cgccgccaga aaagggctcg gtactcctgg ttcccgcacg gtggctgaac tggaatctga
      241 aattctgcaa ctgcgtaagg cgttaaatga agctcgcctt gagcgagata tattaaaaaa
      301 agcaacagcg tattttgcac aggagtcgct gaaaaatacg cgttaatcga acaatggcga
      361 caacaatttc ccattgaagc gatgtgtcag gtatttggtg tatccaggag cggttattac
      421 aactgggtac agcatgaacc ctcagacaga aaacaaagtg atgagcggct aaaactggag
      481 attaaggtgg cacatatccg cactcgcgaa acatatggaa cccggcggct ccagacggag
      541 ctggcagaga atggcatcat cgttggtcgt gaccgactgg cacgtcttcg taaggagcta
      601 aggctacgct gtaagcagaa acgcaagttc agagcgacta cgaactcgaa ccacaatctg
      661 ccagttgcgc caaatctgct gaaccagacg ttcgctccta cagcaccaaa tcaggtctgg
      721 gtggcggacc tgacgtatgt tgccacacag gagggatggt tgtacctcgc tggcatcaaa
      781 gatgtttata cgtgcgaaat tgtcggctac gccatgggag agcgcatgac aaaagagctg
      841 acaggtaaag ctctgtttat ggcgctcagg agccagcgcc cacctgccgg gctaatccac
      901 cactctgatc gaggttcaca gtactgcgca tacgattacc gggtcataca ggagcagttt
      961 ggtctgaaaa catcaatgtc gcgtaaaggt aactgttacg acaacgctcc gatggaaagc
     1021 ttctggggaa cgctgaaaaa tgagagcctg agccactatc gttttaataa ccgggatgaa
     1081 gccatctcag taatacggga atacattgag attttctaca atcgtcagcg tcgtcactct
     1141 cgtctgggga atatctcccc ggcagccttc agggaaaaat atcatcagat ggctgcttaa
     1201 aaaaagaaca aatggtagtg tc
//
LOCUS       DAZGLJ010000474         1216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000474 DAZGLJ010000000
VERSION     DAZGLJ010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.487"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1021)
                     /gene="escV"
                     /locus_tag="ACHK49_005205"
     CDS             complement(<1..1021)
                     /gene="escV"
                     /locus_tag="ACHK49_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFO9265645.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSEALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMIVMAIIPGFPTLVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADL"
     gene            complement(1018..>1216)
                     /gene="cesL"
                     /locus_tag="ACHK49_005206"
     CDS             complement(1018..>1216)
                     /gene="cesL"
                     /locus_tag="ACHK49_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesL"
                     /protein_id="HFO9265646.1"
                     /translation="YNENAFFSFFNALAPERTQGVIEHIFVYDNKLCLSCLLTNIDVF
                     SLMNTFQQHVQIIERVRRMTS"
BASE COUNT          423 a          276 c          221 g          296 t
ORIGIN      
        1 acaaatcagc tcccatagcg tccttattat ttccggttcc aaacgttctt ttcttttgta
       61 atttccaggc gatacccaac aggcaaaccg ccagaaacaa gaagaccaat gtaggaaagc
      121 caggaataat cgccattacg atcatgagtg cggcactgat taaaaaaggg cgactattaa
      181 cagaaacctg ttgaacaata tcacctgcaa ggttttcttc tttttcgctt tcaccgggaa
      241 cacgagtaac aacaaccccc gcagtgacgg aaataataag cgcagggatc tgggcaacca
      301 aagcatcacc gacagacaat acagaaaaca ggctaagtgc ttcactaaat ggcatgtcaa
      361 attgccacat tccaatgagc acgccaccaa acaggttaac taaaacaatg atgataccgg
      421 caatagcgtc cccttttaca aacttcatcg ccccgtccat tgaaccataa aactggcttt
      481 ccttttgcac tcgggagcga agaacttttg cctcaagcgg atcaataacg cctgcgcgca
      541 tatcaccatc aatactcatt tgttttcctg gcataccatc aagggaaaaa cgagcactca
      601 cttcagcaac acgctcagcc ccttttgtaa taaccataaa ttgtacaatg gtgataatgg
      661 taaaaataac caggccaaca acaatattac cgccaacaac aaagttaccg aaggagtaaa
      721 taatgtcacc cgcatcgtga tgcagcagaa taagtctggt tgtactaaca ctgagcgaca
      781 ggcgcatcaa cgtcgtaatc aacaggattg taggaaaaga agttagctca agaggatttt
      841 ttatatagat tgagagcatt aataaaagca acgctgtcga tatattaatc gcgataatga
      901 tatccaccac aactgttggc aatggaataa tcatcatcat tacagccata aagaagaaga
      961 gagccagaat aagatcgtga tatgactctg cttttttaaa tatatttaag agtttattca
     1021 tgatgtcatc ctgcgaactc gttcaataat ctgcacatgc tgctgaaaag tattcattag
     1081 agaaaacaca tcaatattgg ttaacaaaca acttaagcac aatttgttat cgtagacaaa
     1141 aatatgttca atcactccct gcgtccgttc aggggcaagg gcattgaaaa aagaaaaaaa
     1201 cgcattttcg ttatac
//
LOCUS       DAZGLJ010000475         1216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000475 DAZGLJ010000000
VERSION     DAZGLJ010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.488"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1216
                     /locus_tag="ACHK49_005207"
     rRNA            <1..>1216
                     /locus_tag="ACHK49_005207"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          307 a          275 c          374 g          260 t
ORIGIN      
        1 cctgcttcca ggaaaagcct ctaagcatca ggtaacatca aatcgtaccc caaaccgaca
       61 caggtggtca ggtagagaat accaaggcgc ttgagagaac tcgggtgaag gaactaggca
      121 aaatggtgcc gtaacttcgg gagaaggcac gctgatatgt aggtgaagtc cctcgcggat
      181 ggagctgaaa tcagtcgaag ataccagctg gctgcaactg tttattaaaa acacagcact
      241 gtgcaaacac gaaagtggac gtatacggtg tgacgcctgc ccggtgccgg aaggttaatt
      301 gatggggtta gcgcaagcga agctcttgat cgaagccccg gtaaacggcg gccgtaacta
      361 taacggtcct aaggtagcga aattccttgt cgggtaagtt ccgacctgca cgaatggcgt
      421 aatgatggcc aggctgtctc cacccgagac tcagtgaaat tgaactcgct gtgaagatgc
      481 agtgtacccg cggcaagacg gaaagacccc gtgaaccttt actatagctt gacactgaac
      541 attgagcctt gatgtgtagg ataggtggga ggctttgaag tgtggacgcc agtctgcatg
      601 gagccgacct tgaaatacca ccctttaatg tttgatgttc taacgttgac ccgtaatccg
      661 ggttgcggac agtgtctggt gggtagtttg actggggcgg tctcctccta aagagtaacg
      721 gaggagcacg aaggttggct aatcctggtc ggacatcagg aggttagtgc aatggcataa
      781 gccagcttga ctgcgagcgt gacggcgcga gcaggtgcga aagcaggtca tagtgatccg
      841 gtggttctga atggaagggc catcgctcaa cggataaaag gtactccggg gataacaggc
      901 tgataccgcc caagagttca tatcgacggc ggtgtttggc acctcgatgt cggctcatca
      961 catcctgggg ctgaagtagg tcccaagggt atggctgttc gccatttaaa gtggtacgcg
     1021 agctgggttt agaacgtcgt gagacagttc ggtccctatc tgccgtgggc gctggagaac
     1081 tgaggggggc tgctcctagt acgagaggac cggagtggac gcatcactgg tgttcgggtt
     1141 gtcatgccaa tggcactgcc cggtagctaa atgcggaaga gataagtgct gaaagcatct
     1201 aagcacgaaa cttgcc
//
LOCUS       DAZGLJ010000476         1216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000476 DAZGLJ010000000
VERSION     DAZGLJ010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.489"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            115..1216
                     /locus_tag="ACHK49_005208"
                     /pseudo
     CDS             115..1216
                     /locus_tag="ACHK49_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
BASE COUNT          436 a          170 c          289 g          321 t
ORIGIN      
        1 gggcaagatc atgaaaattg tgatgtaaat cacgattttc atcttggctt taacgcctac
       61 aggtgatcaa aatttaacct ttggaataac taaaaagata aaaaaggacg ccaggtgagt
      121 attcaaagtt tgcttgatta tatttcagtg atccctgata tacgacaaca aggaaaggtt
      181 aaacataaat tatctgctat tttgtttctc accgtatgtg cagtaattgc aggtgccgat
      241 gagtggcagg aaattgaaga ttttggacat gaaagacttg aatggctaaa gaaatatggt
      301 gattttgata atggcattcc ggtcgatgac accattgcac gcgttgtgag taacattgac
      361 agtttggcct ttgaaaagat gtttattgaa tggatgcagg agtgccatga aatcactgat
      421 ggtgaaatta tagcaataga tggaaagacc ataagaggct cctttgataa gggaaaaaga
      481 aaaggagcaa tccatatggt gagtgcattc tcgaacgaaa atggtgttgt actggggcag
      541 gtgaaaacgg aagccaaaag taatgagatt acagccattc cagagttgct taacctactg
      601 tatttaaaga aaaatttgat aaccattgat gctatgggct gtcagaaaga tatcgcttcg
      661 aagatcaaag ataaaaaagc agattatctt ctggcagtaa aaggcaatca ggggaaatta
      721 catcatgcat tcgaggaaaa atttcctgta aatgtgtttt ctaattataa aggcgattcg
      781 tttagtacgc aggagataag tcatggaaga aaagaaacac gtttgcatat tgtcagtaac
      841 gtaacgcctg aacttttgtg attttgaatt cgaatggaag ggattaaaaa agctttgtgt
      901 agcattgtca ttcaggcaga agaaagaaga taaatcagca gaaggtgtaa gcatccgata
      961 ttatatttca tcaaaggata tggatgctaa agaatttgca catgctatca gagcgcactg
     1021 gctgatcgag cacagtcttc attgggtgtt agatgtaaaa atgaatgaag atgccagccg
     1081 gataagaaga ggaaacgcag cctaaataat atctggaata aagaagatgg cactgaattt
     1141 attaagggat tgcaaagaca ttaagggtga agaggaaaag aaagaaggct gcgttaaaca
     1201 cagggaaaga tcatca
//
LOCUS       DAZGLJ010000477         1213 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000477 DAZGLJ010000000
VERSION     DAZGLJ010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.490"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            10..279
                     /locus_tag="ACHK49_005209"
     CDS             10..279
                     /locus_tag="ACHK49_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO9265647.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDTDKKLIQCVVRPLQILRADGTWENIGGMK"
     gene            385..>1213
                     /gene="nleH"
                     /locus_tag="ACHK49_005210"
     CDS             385..>1213
                     /gene="nleH"
                     /locus_tag="ACHK49_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase NleH"
                     /protein_id="HFO9265648.1"
                     /translation="MLSPSSVNLGCSWNSLTRNLTSPDSRILSSVRDAAASSDNGAQV
                     KVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNSSPVNRAM
                     ERGAVHSNRPDLPPVDYAPPELPSVDYNSLPVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTNEVRCFNQYYGAGSAEKIYGDNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFIDTTETNVLYDRTRNEFNPIDISSYNISERSWSENQIMQ
                     "
BASE COUNT          369 a          202 c          309 g          333 t
ORIGIN      
        1 gagaatgaca tgaatatact aaaaaaactt atgcagcgtc tgtgtggttg cggaaagcat
       61 gatggccgtg aacacgtgca gtcgcttaca gcacaactgc gactggggcc ggcagacatc
      121 ctggagtcag atgagaatgg cattattccg gagcaggaca gggtaatcac acaggtggtg
      181 atactggata cagataaaaa gctgatacag tgtgtggtaa gaccgctgca aatcctgcgt
      241 gctgacggga cgtgggaaaa tattggcggg atgaagtaac ccgacagctt cacaaaaccg
      301 gagtccggct ccggtttttg ttgtcatgtc atggtgatgt ttgttaagaa agtaaagatt
      361 gattcatttt gaaggttgaa atgtatgcta tcaccatctt ctgtaaattt ggggtgttca
      421 tggaattctt taaccagaaa cctgacttct cctgacagtc gtattttatc ctctgtaagg
      481 gatgcagctg ctagctcgga taatggggcg caagtaaagg tgggcaacag aacatatcgt
      541 gttgttgtca ctgataataa gttttgcgtt acaagagaaa gtcatagtgg ttgctttact
      601 aatctgttgc acaggctggg atggcctaag ggagagatta gcaggaaaat tgaggtgatg
      661 ctgaattcat cgccagtgaa tagggctatg gaaagaggtg ctgttcattc gaatagacct
      721 gatttacctc ctgttgatta tgcaccgcca gagttaccga gtgtggacta taacagcttg
      781 cctgtgcctg gtaatgttat tggcaaaggg ggtaacgctg tagtatatga agatgctgag
      841 gatgcaacaa aagtcctgaa gatgtttact acatctcaaa gcaatgaaga ggtaacaaac
      901 gaagttcgtt gcttcaacca atattatggt gccgggagtg cagaaaaaat atatggcgat
      961 aatggcgata ttattggtat tagaatggat aaaataaatg gagaatcgct tttaaatatt
     1021 tcgtccttgc cggcacaggc tgagcatgcc atttatgata tgtttgacag gcttgagcaa
     1081 aaaggaattc tttttatcga tacaacagag acaaatgtct tatatgatcg cacgagaaat
     1141 gaatttaatc caatagatat atcatcttat aatatttctg aacgttcatg gagtgaaaat
     1201 caaataatgc aat
//
LOCUS       DAZGLJ010000478         1212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000478 DAZGLJ010000000
VERSION     DAZGLJ010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.491"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            159..761
                     /gene="ais"
                     /locus_tag="ACHK49_005211"
     CDS             159..761
                     /gene="ais"
                     /locus_tag="ACHK49_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO9265649.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(800..1210)
                     /gene="nudI"
                     /locus_tag="ACHK49_005212"
     CDS             complement(800..1210)
                     /gene="nudI"
                     /locus_tag="ACHK49_005212"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO9265650.1"
                     /translation="MVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGERIEEA
                     LRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSANREVKI
                     NEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          324 a          273 c          255 g          360 t
ORIGIN      
        1 tattttttct tatttaggct ttgcatttgg caaaattttg aggcattttg ccgacatcgt
       61 aggattttta atattacacc aactgctaat tatcgccaga aatgtcgctc aattttacgg
      121 taattgtctg gttgcgcttg tctataggtg gagtttacgt gttagctttt tgccgctcat
      181 cattgaagtc aaaaaaatat ttcatcattt tactggcgct cgctgcaatt gccggactgg
      241 gtactcatgc cgcctggagt agcaatggtt tgccacgtat cgacaataaa acattggcca
      301 gactggcaca gcagcacccg gttgtcgttt tgtttcgcca tgctgaacgt tgcgatcgtt
      361 caaccaatca atgcttgtca gataaaacag gtattacggt taaaggtacc caggatgccc
      421 gtgaactggg caacgctttt agcgctgaca tccctgattt cgatctttat tccagtaata
      481 ccgtccggac cattcagtcg gcgacctggt tttccgcggg taaaaagctg acggtagata
      541 aacgacttct tcagtgcggt aatgagattt atagtgcaat taaggactta caaagcaaag
      601 cgcctgataa aaatatcgtt attttcaccc ataatcattg cctgacatat attgctaaaa
      661 ataagcgtga tgcgacattt aaacctgatt atctggatgg tttagtcatg catgtggaaa
      721 aaggcaaagt ttatctggat ggggaattcg ttaaccacta aattttcaat ctgacagcca
      781 gtgatggctg tcatcattgt taaagaagac ctttcaaacg taacgttttt cgggtggcaa
      841 catttaaatc ataatgcacc agatcttcag gttttaccca cgcgtagtcc tgaaactctt
      901 cgtttatttt cacttctcgg ttggcagaaa cgcagtcaaa aatcaggtaa atcatataaa
      961 tctcttcctt gcgaccatct gcatacgtct tggtgcgaat atcatcgctg aaggtccacg
     1021 gcgtgatttc tgtcaaaagc agctgttctc ccagttcttc gcgaatttcg cggcgtagtg
     1081 cctcttcaat tcgttcgcca gactccacgc cgccacccga aagcgcccat tgaccgggga
     1141 aaacgccgcg atcgtcggcc attttacaca gcaaataagc accatcattt tgaatcaaag
     1201 ggcatacaat ag
//
LOCUS       DAZGLJ010000479         1198 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000479 DAZGLJ010000000
VERSION     DAZGLJ010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.492"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            143..1165
                     /gene="wzy"
                     /locus_tag="ACHK49_005213"
     CDS             143..1165
                     /gene="wzy"
                     /locus_tag="ACHK49_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O26 family O-antigen polymerase"
                     /protein_id="HFO9265651.1"
                     /translation="MYYIIFVMVLGLWIIAFNYARANKLSNLVIVLIITTLFIINRQN
                     QDYEAYVDIFNVNELYAEIGYRWLIYGVKYLGGTHEVIIGLLGLFLGTTFLRLIQYSK
                     YTAFGLLLYMLCPMPIDIVQIRNTFLFLFVINSLIELEKEHKFNSLCFVFMAPLFHSL
                     GIVYVLAWVIIQFRTWRGYNKLMVFGLVLSFIVVPLLIKMLILLFNTRTLHAYIADGI
                     KVHSLIIWAGPYLFDLFLLCYFRKKIVITDLHTKQWIDIIFSLMLFLSVFSPLLLYID
                     EINRIFRNALLLKYLVMMSIAQYLSRPTRYILYSYLLLFTFALSIYYTLQIDYDYIVF
                     GLPYSL"
BASE COUNT          348 a          170 c          201 g          479 t
ORIGIN      
        1 ctgcaattgc ttatgtattt gctgagttta tcttacttat tctcatactt agaatttata
       61 aagtgaaacg attataattc ccggtaagaa ctgatagtgc aaaatcatca tggctataga
      121 ctgaataaat tgcggggaaa gaatgtatta catcatattt gtgatggttt taggtttatg
      181 gattattgca ttcaactacg ctagagcaaa caagctaagt aatttggtaa ttgtgcttat
      241 tattactact ttatttatta ttaataggca gaatcaagac tatgaagcgt atgttgatat
      301 atttaatgtc aatgaacttt atgccgagat tggttatcgc tggttaattt atggtgttaa
      361 gtatttaggc ggtacccatg aagtcataat tggcttgctg ggtttattcc ttgggaccac
      421 attcctacga ttaatacaat acagtaagta tacagcattt ggtttgctgc tttacatgtt
      481 gtgtccaatg cctattgaca ttgttcaaat caggaataca tttctttttt tatttgtcat
      541 aaattctctt atcgagttag aaaaagagca taaatttaac tctctttgtt ttgtctttat
      601 ggcacctctt ttccatagtt taggcattgt ttatgtattg gcgtgggtaa taatccaatt
      661 tcgtacctgg aggggttata ataagttaat ggtttttgga ctggtgctgt cttttattgt
      721 agtgccactc ttgattaaaa tgttaatttt attatttaac actaggacgt tacatgcata
      781 tatagctgat ggcattaagg ttcattctct tattatatgg gcaggtcctt atctttttga
      841 tctttttctg ttatgttact tcagaaaaaa aatagtaata actgatttac atactaaaca
      901 atggattgat ataatattct cgctaatgtt atttctttcc gtattttcac cactactttt
      961 atatatcgac gaaataaata ggatttttcg aaatgcgcta ttactgaagt atctggtaat
     1021 gatgtctatc gcacagtatt taagtagacc gaccagatat atactttatt catatttact
     1081 gttattcact tttgcattat caatatatta tacactccaa atcgattatg attacattgt
     1141 atttgggtta ccatactcat tatgatactt atgaggatat atgttttatt ttatctgt
//
LOCUS       DAZGLJ010000480         1198 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000480 DAZGLJ010000000
VERSION     DAZGLJ010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.493"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..277)
                     /gene="chbB"
                     /locus_tag="ACHK49_005214"
     CDS             complement(<1..277)
                     /gene="chbB"
                     /locus_tag="ACHK49_005214"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO9265652.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGV"
     gene            complement(576..914)
                     /gene="osmE"
                     /locus_tag="ACHK49_005215"
     CDS             complement(576..914)
                     /gene="osmE"
                     /locus_tag="ACHK49_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO9265653.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1116..>1198
                     /locus_tag="ACHK49_005216"
     CDS             1116..>1198
                     /locus_tag="ACHK49_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265654.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSV"
BASE COUNT          309 a          292 c          258 g          339 t
ORIGIN      
        1 gcacgcctaa accatcgact ttgccataaa gcagcgagtc aattacttca accggtttgt
       61 tgggtaacaa acgctggatt tcgggcaaca tataagcaat ctgcggccct aataacacga
      121 catcggcatt ctgacctttt tcaccagcca gtgtttccgg aaatgcttca ataatgaccg
      181 gaacttcata tttttctgcc tgtgcgcgca tttttgatac cagtaaagag gtagacatgc
      241 ccgcagaaca aaacagataa atgtgtttct tttccataaa actgccctca tcgacgatta
      301 tctgtcagcc agacactccg caagccttaa cctgcttcca tgctctgggt aacttgcgaa
      361 accaacatga ctttttttgt ggctgaaagg agtatacggt atagacctga gcaatagtat
      421 ttctttgacc ttataaattg tttctccttg acctgtggtt atgaccctct tcacatttcg
      481 ggcaaataat tcgcgaagat aattaagaac tcaggccaaa aaaaaccggc gcaatggccg
      541 gtttccgttg ttactcaagc tttcagacga attgattact tcgcagcctg tggatcagtg
      601 tcgtattcag cacaggtctg ataaccggag ttgatgacat gtccggtatc atctaacgcg
      661 acaaagtagg tttctgcttt accatcacgt tgacccagga tgtaggtctg gcaagtaccg
      721 cgagcatgga tcatgctcac ttcagacgaa ggtttacccg caatttgtgc aacctgcgcc
      781 cggctcatgc cttttttgac gtctttcacc acaggctgta caaactggtc tttggtacga
      841 tcataagccg tacaacccgc cagcatggtt aataccgccg ctgcactcag aattcctgcc
      901 atattcttgt tcatattccg tcctcttgtt tatcagcgtg ttagataagc ctggaataca
      961 ttgggcgctt tttcaagccc gtgaacgaaa cggctccgct ttcagaggat tcctgtatga
     1021 cgttttaacc accattcagc ccgctgtcgc ttgtcgtttc agtagcaacg ggttagcttt
     1081 aaggaagttt tgtcttttct gtctggaggg gttcaatgac attgcaacaa caaataataa
     1141 aggcgctggg cgcaaaaccg cagattaatg ctgaagagga aattcgtcgt agtgtcga
//
LOCUS       DAZGLJ010000481         1197 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000481 DAZGLJ010000000
VERSION     DAZGLJ010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.494"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..311
                     /locus_tag="ACHK49_005217"
     CDS             <1..311
                     /locus_tag="ACHK49_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO9265655.1"
                     /translation="NILKGEQRRVSGSARVSLKRHIWLRDAGQCCLCGRVVDLCDSEL
                     DHRIALQFGGGNEETNLWTLCTECHRQKSVSETASGMPDPTLPEVSGGHGRADDIIGL
                     "
     gene            603..1166
                     /locus_tag="ACHK49_005218"
     CDS             603..1166
                     /locus_tag="ACHK49_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO9265656.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEPEDKPRRREAAAIPQPDETNPEMPPPAVMS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
BASE COUNT          293 a          292 c          366 g          246 t
ORIGIN      
        1 tgaatattct gaagggtgaa cagcgtcggg tcagtggcag tgcacgtgtt tccctcaagc
       61 gtcatatctg gctcagggat gccgggcagt gctgtctctg tggtcgtgtg gttgacctct
      121 gtgacagtga actcgatcac cgaattgcac ttcagttcgg tggtggtaat gaggagacga
      181 atctctggac gctctgtacc gaatgccatc gccagaagtc agtcagtgaa acggcgagtg
      241 gtatgccgga cccgacgctg ccggaggtgt ccggaggtca tggcagggca gacgatatca
      301 tcggactgtg acccgccccg ggggggggga tcatccggcg aaaaaaacga tcgccccgga
      361 caccgccccc cgtctcacgc agagaaaaaa ttcccgtttc aggccagtta acatgttaac
      421 tggctgtccg ggcatttttg cggtttttat ctttattatt cagtttgttg cgtggaaaaa
      481 atgttaacag gctttttcag caaatgttaa ccaggcagca gttaacattt gcggcatgag
      541 acgccgggaa aaatgggctg aaccataccc ggctgagtgc gttatggacc cgggaggagg
      601 ctgtgctgac aacgcaaaaa cgaaaatttg cgctggcgct catgtccggg aaaaacaaaa
      661 cagcgtcagc cattgccgct ggttattcgg cgaagaccgc cagggttaaa ggctcgcagc
      721 tggcaaaaga tccggaggtg cttgcgttta tagcccgtaa acaatgcgag acggtggagg
      781 tggatgaggt tcctgtttac cggcagaaaa aatcagagcc ggaggataaa ccccgtcgcc
      841 gtgaggcggc tgcaatacca cagccggacg aaacaaatcc ggagatgcca ccgcccgcgg
      901 tgatgtctcc tggtattgaa tatatggagg acggtcttcc cgatccggtg aaagcgatgg
      961 ggcgtcttct ggtggagaac attaataccg accccaggct ggcgctggat gcggcttata
     1021 agctggcgca gttcacgcac cacaaaaaag gggatgccgg taaaaaatcg gcaaaaggtg
     1081 acgcggcgaa aaaagcggct aaccgttttg cggtgccacc accaccccgc ctggtggtga
     1141 ataatgataa tgagggcaac ggatgatacc tgtgtggagc acggcctgcc cggactg
//
LOCUS       DAZGLJ010000482         1196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000482 DAZGLJ010000000
VERSION     DAZGLJ010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.495"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1014
                     /locus_tag="ACHK49_005219"
     CDS             <1..1014
                     /locus_tag="ACHK49_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9265657.1"
                     /translation="GFNVGSPQQAGISELVLRAGNGNPKGITGALWQRTSTGFTNFAW
                     VNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPEGLTDGTVYSLY
                     TPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIK
                     GKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSG
                     STSSAGAHSHVDGRRFNTSTFKDTYQYGSTAVGSNSYQVQGAVGLGIGTMANTNSAGA
                     HTHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIV
                     RLA"
     gene            1014..>1196
                     /locus_tag="ACHK49_005220"
     CDS             1014..>1196
                     /locus_tag="ACHK49_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO9265658.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSD"
BASE COUNT          312 a          296 c          294 g          294 t
ORIGIN      
        1 ggatttaacg tggggtcacc tcaacaggca ggtatatctg aacttgtttt gcgtgcaggt
       61 aatggtaatc cgaaggggat tactggtgct ttatggcagc gcacatcgac agggtttaca
      121 aattttgcct gggtcaatac atctggtgat acttacgata tttacgttgc aatcggaaat
      181 tatgcgactg gtgtaaatat tcaatgggat tataccagta atgccagcgt gacgattcat
      241 acgtcaccag catattctgc taataagccg gaagggttaa cggacggtac agtttattca
      301 ctctatacgc catcagagca gttttatccg cctggcgcac caatcccgtg gccatcagat
      361 accgttccgt ctggctatgc cctgatgcag gggcagactt ttgacaaatc tgcatacccg
      421 aaacttgcag ccgcttatcc gtcaggcgtg atccctgata tgcgtggctg gacgattaag
      481 ggcaaacccg ccagtggtcg tgccgtattg tctcaggaac aggacggcat taaatcgcac
      541 acccacagcg ccagcgcatc cagtacggat ttggggacga aaaccacatc gtcgtttgat
      601 tacggcacta aatccacgaa taacaccggg gctcatacgc acagtctgag cggctctaca
      661 agctcggccg gagcacactc gcacgttgat ggtcgcaggt ttaatacaag cacttttaaa
      721 gacacctatc agtatggttc tacagctgtc ggaagtaact cataccaggt acaaggggcg
      781 gttggtctgg gcattggcac gatggcgaat acgaactctg ctggcgcgca tacccactca
      841 ctgtccggca ctgctgcaag cgcaggtgca cacgcacata ctgtcggtat tggtgctcat
      901 acgcactccg ttgcgattgg ctcacatgga cacaccatca ccgttaacgc tgctggtaac
      961 gcggaaaaca ccgtcaaaaa catcgcattt aactatattg tgaggcttgc ataatggcat
     1021 tcagaatgag tgaacaagca cggaccataa aaatttataa tctgctggcc ggaactaatg
     1081 aatttattgg tgaaggtgat gcatatattc cgcctcatac aggtctgcca gcaaacagta
     1141 ccgatattgc accaccagat attcctgctg gctttgtggc tgttttcaac agtgat
//
LOCUS       DAZGLJ010000483         1192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000483 DAZGLJ010000000
VERSION     DAZGLJ010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.496"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..944
                     /locus_tag="ACHK49_005221"
     CDS             <1..944
                     /locus_tag="ACHK49_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO9265659.1"
                     /translation="QQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQC
                     GLTVEQLARQVEPEYTPARKVHFYHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEE
                     NPYYLHQPYRLPGQQHDEESGLYYNRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNP
                     IIRMDPLGLYNLYQLLYDVWHDDSYGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEM
                     LSDICIYATACGHAGIGGGINAAITYSETKSLPASGVSNSVGVTVGGGVGGHFAYTYV
                     VDVDNPESSTESVGIGAGVDASVMTLACRTWQECWVN"
     gene            956..1141
                     /locus_tag="ACHK49_005222"
     CDS             956..1141
                     /locus_tag="ACHK49_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265660.1"
                     /translation="MKKYIFLVLFLLCGCSPVDIEPARQEVNIFRGLYQTGKYRDIYK
                     ITSSDFQTATSEKNLST"
BASE COUNT          335 a          219 c          335 g          303 t
ORIGIN      
        1 tgcagcagga agggagcgag gacgggcacg gcgtggtatt tccggctgaa ctggtgcggc
       61 tgctggacag gctggaggaa gaaatccggg cagaccgcgt gagcagtgaa agccgggcgt
      121 ggcttgcgca gtgcgggctg acggtggagc aactggccag acaggtggag ccggaataca
      181 caccggcgcg aaaagttcat ttttaccact gcgaccaccg gggcctgccg ctggcgctca
      241 tcagcgaaga cggcaatacg gcgtggcgcg gggagtatga tgaatggggc aaccagctta
      301 atgaagagaa cccgtattac ctgcaccagc cataccgtct gccggggcag cagcatgatg
      361 aggaatcagg gctgtactat aaccggaacc ggtactatga cccgctacag gggaggtata
      421 ttacacaaga ccccattggg ctggcggggg gatggaacct gtataattac ccactgaatc
      481 cgataataag gatggatcct ttgggtttgt ataatttata tcaattatta tatgatgttt
      541 ggcatgatga ttcatatgga acatcatcaa ttgatattac tggcagtgga gatctaatat
      601 cattaggtgg tcatgcagga cttggcgttg cgtttgctaa aaagaaaggt gaaatgttat
      661 ctgatatttg tatttatgct acagcatgcg gacatgcagg aattggtggt gggataaatg
      721 cggctatcac atattctgag accaagtctt tacctgcatc gggagtcagc aattcagtag
      781 gtgtaacggt tggcggcgga gttggggggc attttgcgta tacttatgta gtggatgttg
      841 ataatccaga atcatcgaca gaatctgttg gtatcggtgc aggtgttgac gcttcagtta
      901 tgactctggc ttgtagaacg tggcaagaat gctgggtcaa ttaacaggag gaaatatgaa
      961 aaaatatata tttttagttt tatttttatt gtgtggttgt tctccagtgg acatagagcc
     1021 agctagacaa gaagttaata tctttcgtgg tttatatcag acagggaaat accgtgatat
     1081 atataaaatc acttcaagtg attttcagac tgccacatct gaaaaaaatt tatcgacata
     1141 atgaacgaag caaaagaaaa agatcttggg acttataaaa aatcaaccct ta
//
LOCUS       DAZGLJ010000484         1187 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000484 DAZGLJ010000000
VERSION     DAZGLJ010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.497"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            141..1100
                     /locus_tag="ACHK49_005223"
     CDS             141..1100
                     /locus_tag="ACHK49_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFO9265661.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
BASE COUNT          419 a          226 c          219 g          323 t
ORIGIN      
        1 ataataaaaa ccgcaataaa aaacgaataa atcaattgtc tcacgcaatt ataaaacatc
       61 atattgatta cgcaccttgt attacaaact catgtatgta aaatacgcgc accattcaaa
      121 aaaaaggaag acaataacat atgaaaaaaa gtgtcatcgc tggcgtcttt attgctctgt
      181 catttaccac gtgttcagct atcgcgaaca gccttgcatt atcattagca aatgatgatg
      241 cagggaagtt tcaaccaata cttaatgata tttatggcaa taaacatgaa aacagagatg
      301 attactcaca aggcttattt ctgggatata gccacgatat ctcagactcg agccaattat
      361 ctctccatat tgcgcaagat atttactctc catcaggcag taataaaaga cacaacacag
      421 ctgtaactgg agacagagct tttagtgcat acactcacac tggtattgaa tggaactccc
      481 ttgcgaatga ctggattcgc tatcgattag gtactgacat aggtgttgtt ggccccgacg
      541 caggcggtca gaaagtacaa aataaagctc atgagattat tggggcagaa aaatatcatg
      601 catgggatga tcaaatagag aatcgctacg gttatactgt aaaagggatg ctatccatga
      661 caccaagtat ggatatttta ggtgctaatg ttggattata ccctgaagtt tctgctgtta
      721 ctggaaactt atttcaatat gtagcatatg gcgcaaccat tgccattggt aatgataaaa
      781 ccttcaattc ggataatggc tttggtctgc tggctccccg tggtttaatg catatgtccg
      841 atacaagcgg attcaaatac aagatttttg caggtatgga aagacgagat gtcaatcgca
      901 actatactct cgaaggaaaa acaatacaga cgaaacaaac aacagtatcg ctaaacaaaa
      961 ctgttgatga atatcaagtt ggcgcaacaa ttgggtatgc acctgtagcc ttcacactag
     1021 catttaataa agtaacatca gaattcaaga caggggatga ctattcattt ataaatggag
     1081 caatcacctt ctttttttaa ctgaattgaa ttcaatcaaa ataacataag tccaacaaaa
     1141 acataaagtg cgaaatgaat gccagctcca tttatttcgc actataa
//
LOCUS       DAZGLJ010000485         1185 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000485 DAZGLJ010000000
VERSION     DAZGLJ010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.498"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..449
                     /locus_tag="ACHK49_005224"
     CDS             <1..449
                     /locus_tag="ACHK49_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system protein SepQ"
                     /protein_id="HFO9265662.1"
                     /translation="PHSNTIFFDGYIAPGWTDYPVTELTVGDSLRLYHVDDSKERECW
                     LVINNPLATVKLCDNNLFIADVQAADLLCTISNEAVMDRIYCVIGTIHVDIHMLRNAK
                     KDDIIDSTGYHLFGGCRLIRNNTTIAYGSIVKINEDFYFTVSLVCD"
     gene            480..995
                     /locus_tag="ACHK49_005225"
     CDS             480..995
                     /locus_tag="ACHK49_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspH"
                     /protein_id="HFO9265663.1"
                     /translation="MSSSLSGIILNTSLTSHHMSWGKFSRTLHVLNEDIVDEIQLKAR
                     TQQRHHRVYSEMADYSVVSFRGKEYSVRFIKDYKTDNYVYKVQKMTQESGCLSRLFSF
                     LSGGVTKALESKLNERHITPLSPTWFPRTPLEGVLTERGLSSLLRRVQSTENLDYSNA
                     MISNSFSNSVV"
BASE COUNT          346 a          190 c          252 g          397 t
ORIGIN      
        1 ttccacattc taataccatt ttctttgatg gatatattgc tcctgggtgg actgattacc
       61 cggttacaga gctgactgtt ggtgatagtt taaggctcta tcatgttgat gacagcaaag
      121 aaagagagtg ttggcttgtt attaataacc ctttagcaac cgtaaaactt tgtgataata
      181 atttatttat tgcggatgtc caggctgcag atttgttatg tactatcagc aatgaggctg
      241 taatggatcg catttattgt gttattggaa caattcatgt tgatatacat atgttgcgca
      301 atgccaaaaa agatgatatt atcgattcaa ctggatatca tctttttggt gggtgccggt
      361 taatacgcaa taatacgact atagcatacg gaagcattgt aaaaatcaac gaagattttt
      421 attttactgt tagtttagta tgtgattaat caggaataag ttatataggg agatgtatta
      481 tgtcgtcatc gttatcagga ataatactca atacttcgct gacaagtcac catatgtcat
      541 ggggcaagtt tagcagaact cttcatgtat tgaatgaaga tatcgtagat gaaattcagc
      601 ttaaagcccg aacacaacaa cgccaccatc gagtatattc agaaatggcg gattactccg
      661 ttgtctcctt tcggggcaaa gaatattctg ttcgttttat taaagattac aaaacagata
      721 attatgtgta caaagtacaa aagatgactc aggaaagtgg gtgtctctca agattattta
      781 gctttctttc tggcggtgtg actaaggctt tggaaagtaa actaaatgag aggcatatca
      841 ctccactttc tccaacctgg tttcctcgaa ctccactgga gggggtctta acggaaagag
      901 gattatcttc tttgttacgt cgcgtacagt ctactgaaaa ccttgattat agtaatgcta
      961 tgatatcaaa ctctttctct aactccgtag tgtgattatc atttgagctt tcctcctggt
     1021 ttaactccag aataattgtg attctggagt ttttctgttt gttttatgtc gataatacat
     1081 tgtttttagg aaaatatggg atgaatattc agagtgagtt tggctggcag aagtcagaga
     1141 ttctgtgatg aggaggggat tgagctgaat ggatgaattc cttcc
//
LOCUS       DAZGLJ010000486         1181 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000486 DAZGLJ010000000
VERSION     DAZGLJ010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.499"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            179..472
                     /gene="iss"
                     /locus_tag="ACHK49_005226"
     CDS             179..472
                     /gene="iss"
                     /locus_tag="ACHK49_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFO9265664.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(563..745)
                     /gene="rzoD"
                     /locus_tag="ACHK49_005227"
     CDS             complement(563..745)
                     /gene="rzoD"
                     /locus_tag="ACHK49_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO9265665.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(962..>1181)
                     /gene="rrrD"
                     /locus_tag="ACHK49_005228"
     CDS             complement(962..>1181)
                     /gene="rrrD"
                     /locus_tag="ACHK49_005228"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO9265666.1"
                     /translation="IPETTRGALYSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRR
                     WTYAGGKQWKGLMTRREIEREICLWGQQ"
BASE COUNT          301 a          282 c          269 g          329 t
ORIGIN      
        1 ctaaaagggt aatggacaga tagagcggtt tatttcattc cacaggattc tgagtgcccc
       61 cctcctccaa taggctgagc atccacctat atagttttaa ttttcatcaa tccatttaac
      121 tatcgtttaa ttgttgtcac ataggattct gccgttttta acaatgcagg ataataagat
      181 gaaaaaaatg ttgttttctg ccgctctggc aatgcttatt acaggatgtg ctcaacagac
      241 gtttactgtt ggaaacaaac cgacagcagt aacaccaaag gaaaccatca cccatcactt
      301 cttcgtttcg ggaattggac aggagaaaac tgttgatgca gccaaaattt gtggcggcgc
      361 agaaaatgtt gttaaaacag aaacccagca aacattcgta aatggattgc tcggttttat
      421 tactttaggc atttatactc cgctggaagc gcgtgtgtat tgctcacaat aattgcatga
      481 gttgcccatc gatatgggca gctctatctg cactgctcat taatatacgt ctgggttcct
      541 tccagttgtt tttgcatagt gatcagcctc tctctgaggg tgaaataatc ccgttcagcg
      601 gtgtctgcca gtcgggggga ggctgcatta tccacgccgg aggcggtggt ggcttcacgc
      661 actgactgac agactgcttt gatgtgcaac cgacgacgac cagcggcaac atcatcacgc
      721 agagcatcat tttcagcttt cgcatcagct aactccttcg tgtattttgc atcgagcgca
      781 gcaacatcac gctgacgcat ctgcatgtca gtaattgccg cgttcgccag cttcagttct
      841 ctggcatttt tgtcgcgctg ggctttgtag gtaatggcgt tatcacggta atgattaaca
      901 gcccatgaca ggcagacgat gatgcagata accagagcgg agataatcgc ggttactctg
      961 ttcattgctg accccacaaa cagatttcac gctcaatctc acgacgagtc atgagacctt
     1021 tccattgctt accgccagca tatgtccagc gacgtagctg atcacatgcg cctttgatat
     1081 cgccctggtt tattttgcga agaagcgtcg atgttctgaa attgccagca cccacgttgt
     1141 aaacgaatga gtaaagagcg ccgcgcgttg tttccggtat a
//
LOCUS       DAZGLJ010000487         1177 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000487 DAZGLJ010000000
VERSION     DAZGLJ010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.500"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..129)
                     /locus_tag="ACHK49_005229"
     CDS             complement(<1..129)
                     /locus_tag="ACHK49_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO9265667.1"
                     /translation="MHRDSERSRRIRGWVIMKKDLKTLALARLSGFRHKTVKVPEWG"
     gene            complement(96..812)
                     /locus_tag="ACHK49_005230"
     CDS             complement(96..812)
                     /locus_tag="ACHK49_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO9265668.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRMKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(879..>1177)
                     /locus_tag="ACHK49_005231"
     CDS             complement(879..>1177)
                     /locus_tag="ACHK49_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO9265669.1"
                     /translation="QVYPYVVPLLDGRPSVALPWVVFSLISSVSADVMGGQAESSVSV
                     QIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENRCYRATLEFQVTV"
BASE COUNT          269 a          364 c          284 g          260 t
ORIGIN      
        1 accccattcc ggcaccttca ccgttttatg acgaaaccct gacagtctgg ccagcgccag
       61 cgttttcaga tcctttttca tgatgaccca tccccttatc cggcggctgc gctcactgtc
      121 acggtgcatt caacagacgt cacactctgt gctttctctg ccgaatcggt caccacgcag
      181 gtatatttcc ccgcatcagc ggactgcgca cctggcttac tgaaggtgtc tgtcgtctgc
      241 ccgtcaaccg gctgaccatc cttcttccag gcgtatttat acggcggcgt tcccccgttg
      301 gcactgactg acattgtcag cagcgcacct gtattcacgg taagtgtttt atccagattt
      361 ttcacaaacg ccagcggtac cacaaaggac accggtttgc ctttcatacg cagtgaaaac
      421 gttgctgcca ccacgccgtt ggtaccggat gaccaggtgt gctgacgcac ttccgccagg
      481 aacttaaagc ccttaccgga cggaaacagc accttaaacg catacaacgc gtcattgtca
      541 taggcatcac gcagggcgtt ctgggcctga ttcagataaa aattacccga catggaaatc
      601 tcagacgacg cccccagacc gttgatgttc tcctgctcgg tggagcagag cgtggtcaca
      661 tcaatatcct gtttctgacc ggcggtgaac tggacttcct tgatggtgca gtccaggcgc
      721 agatattccg ccttatccat agtttcagca gtcgccgggg cagatgaaat catcacctgc
      781 gtcagctgtg agcgttcata caaagcagac attctgcctc ctgataataa aaaacccgca
      841 cgcggcgggg tatgggtttt gtagaaaaaa agaaaaagtc acaccgtgac ctgaaactcc
      901 agggttgcac ggtaacagcg gttttccgga atatagtcct gcatttcact gacggatccc
      961 ggggccagca gcattatggc ttcacgggcg tcctgacgta tctgacgcgc ctgcgtcaca
     1021 gtcccggcat aaacgtctat ctgcaccgac actgaggact ccgcctgccc gcccatcacg
     1081 tccgccgaca ccgatgaaat caggctgaaa accacccacg gaagcgccac cgacggcctg
     1141 ccatccagca gggggaccac atacgggtac acctgcc
//
LOCUS       DAZGLJ010000488         1170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000488 DAZGLJ010000000
VERSION     DAZGLJ010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.501"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..689)
                     /gene="escT"
                     /locus_tag="ACHK49_005232"
     CDS             complement(<1..689)
                     /gene="escT"
                     /locus_tag="ACHK49_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFO9265670.1"
                     /translation="MNEIMTVIVSSFFCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIVLKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISSIFNPSISDSSSITGVILYQFITVIFVIHGGIQSVLDKLYLSYEILPLQAD
                     IAFNRALVDFLFSLWDSFIKLMLSFSIPMIIGIFLCDMGFGFLNKTAPQLNVFTLSLP
                     VKSLIAIFILL"
     gene            complement(689..958)
                     /gene="escS"
                     /locus_tag="ACHK49_005233"
     CDS             complement(689..958)
                     /gene="escS"
                     /locus_tag="ACHK49_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFO9265671.1"
                     /translation="METGYFVQLCVQTLWIIFILSLPTVIAASVVGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            complement(958..>1170)
                     /gene="escR"
                     /locus_tag="ACHK49_005234"
     CDS             complement(958..>1170)
                     /gene="escR"
                     /locus_tag="ACHK49_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFO9265672.1"
                     /translation="FTMGQLEAAFKIGFLLYLPFIAIDLIISNILLALGMMMVSPVTI
                     SIPFKILLFILVGGWQKLFEFLLVVN"
BASE COUNT          443 a          216 c          195 g          316 t
ORIGIN      
        1 aataacaata taaagattgc tatcaaactt tttaccggca gtgataatgt gaatacgttt
       61 agctgaggcg ctgttttatt gagaaaccca aaccccatat cacataagaa gataccgata
      121 atcatgggaa ttgaaaatga taacatcagt ttaataaatg aatcccatag cgaaaataaa
      181 aaatctacta aagcacgatt gaatgcaata tcggcttgta atggtaatat ctcgtaggat
      241 aaatacaact tatccagaac gctttgtatc ccaccatgaa taacaaagat cacagtgata
      301 aattggtata aaataacacc agtgatagaa gatgaatcac ttatagacgg gttaaaaatt
      361 gaagatattg ttgaacctct taaagtatca ataatctgcc cagccgcatc tattgcccaa
      421 aaaagaatag taaatgataa cccaatgaaa aagcctatac ttatttcttt tagcactatg
      481 cctgtcaact gaaaaattcc ggatggtaat ttttccgaaa atgtatagtt ctctactata
      541 attggaagtg taaacgcaat cataattgag ttgcgtataa agttactgag caaaacacct
      601 gttgaaaata ttggtagaat aataaacatc cccagcggcc tgagtataca aaaaaatgat
      661 gatactatga ccgtcattat ttcattcatt agccattcac cttcggaatc atttcaaata
      721 ttatcgacga gaagttgatg attgttgttc ccatccagtg ataagtcagg gcaagcgtag
      781 caaacactgc tattattttt agcaaaaaag gcaatgtttg atcctgtaac tgcgttatag
      841 cctgaaccag gctaataata atgccgacaa cagaggccgc tatgactgta ggcagtgaga
      901 ggataaatat tatccaaagc gtttgcacac ataattgaac aaaatatcca gtttccatca
      961 attcaccacc aacagaaatt cgaataattt ttgccagcct ccaacaagaa tgaataacaa
     1021 gatcttaaaa ggaatcgaaa ttgttactgg cgataccatc atcataccca aggccaataa
     1081 gatattggaa atgatcaaat ctatcgcaat aaaaggtaaa tagagcaaaa aacctatctt
     1141 gaatgcagct tcaagctgcc ccatcgtgaa
//
LOCUS       DAZGLJ010000489         1169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000489 DAZGLJ010000000
VERSION     DAZGLJ010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.502"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1169
                     /locus_tag="ACHK49_005235"
     CDS             <1..>1169
                     /locus_tag="ACHK49_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO9265673.1"
                     /translation="NYYLPKESAYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARV
                     GYSKMLLGVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGK
                     KHRDNTLTMKRFTNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFL
                     GDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDK
                     ETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGIWTRDGILWFSSS
                     GEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIG
                     ERPDAEVMAERKEHYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDR"
BASE COUNT          274 a          294 c          344 g          257 t
ORIGIN      
        1 ccaattacta tctcccgaaa gaatccgcat accaggaagg gcgctgggaa acactgccct
       61 ttcagcgggc catcatgaat gcgatgggca gcgactacat ccgcgaggtg aatgtggtga
      121 agtctgcccg tgttggttat tccaaaatgc tgttgggtgt ttatgcctac ttcatagagc
      181 ataagcagcg caacaccctt atctggttgc cgacggatgg cgatgccgag aactttatga
      241 aaacccacgt cgagccgacc atccgtgata ttccatcgct gctggcgctg gccccgtggt
      301 atggcaaaaa gcaccgggat aacacgctca ccatgaagcg tttcaccaat gggcgtggtt
      361 tctggtgcct gggcggtaaa gcggcaaaaa actaccgtga aaagtcagtg gatgtggcgg
      421 gttatgatga acttgctgcc tttgatgatg atattgaaca ggaaggctct ccgacgttcc
      481 tgggtgacaa gcgtattgaa ggctcggtct ggccaaagtc catccgtggc tccacgccca
      541 aagtgagagg cacctgtcag attgagcgtg cagccagtga atccccgcat tttatgcgtt
      601 ttcatgttgc ctgcccgcac tgcggggagg agcagtacct taaatttggc gataaagaga
      661 cgccgtttgg cctcaaatgg acgccggatg atccctccag cgtgttttat ctctgcgaac
      721 ataatgcctg cgtcatccgc cagcaggagc tggacttcac tgatgcccgt tatatctgcg
      781 aaaagaccgg gatctggacc cgtgatggca ttctctggtt ttcgtcatcc ggtgaagaga
      841 ttgagccgcc ggacagtgtg acctttcaca tctggacggc gtacagcccg ttcaccacct
      901 gggtgcagat tgtcaaagac tggatgaaaa cgaaagggga tacgggaaaa cgtaaaacct
      961 tcgtgaacac cacgctcggt gagacgtggg aggcgaaaat tggcgaacgt ccggatgctg
     1021 aagtgatggc agagcggaaa gagcattatt cagcgcccgt tcctgaccgt gtggcttacc
     1081 tgaccgccgg tatcgactcc cagctggacc gctacgaaat gcgcgtatgg ggatgggggc
     1141 cgggtgagga aagctggctg attgaccgg
//
LOCUS       DAZGLJ010000490         1169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000490 DAZGLJ010000000
VERSION     DAZGLJ010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.503"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            36..>1169
                     /locus_tag="ACHK49_005236"
     CDS             join(36..324,324..>1169)
                     /locus_tag="ACHK49_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
                     /protein_id="HFO9265674.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTSAERQRLKELERENRELRRSNDILRQASAYFCEGGV
                     RPPLEKMMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQHDDWLK
                     REIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTIS
                     RKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSS
                     METTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLL"
     misc_feature    317..433
                     /locus_tag="ACHK49_005236"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          284 a          275 c          358 g          252 t
ORIGIN      
        1 tggagactaa actccctgag aaagaggtaa acaggatgac taaaaatact cgtttttccc
       61 ccgaagtccg tcagcgggcg attcgtatgg ttctggaaag tcaggatgaa tatgactcac
      121 agtgggcggc aatttgttcc attgccccaa agattggctg tacgccggag accctgcgtg
      181 tctgggttcg ccagcatgag cgggataccg ggggcggtga tggtgggctc accagcgctg
      241 aacgtcagcg tctgaaagag ctggaacgtg aaaatcgtga actgcgccgc agtaacgata
      301 tccttcgcca ggcttccgct tattttgcga aggcggagtt cgaccgcctc tggaaaaaat
      361 gatgccactg ctggataagc tgcgtgagca gtacggggtc ggaccggtat gcagcgaact
      421 gcatattgcc ccgtcaacgt attaccattg tcagcaacag cgacatcatc cggataaacg
      481 cagtgcccgt gcgcagcacg acgactggct gaagagagag atacagcgcg tatacgatga
      541 aaatcatcag gtgtacggtg tgcgtaaagt ctggcgtcag ttgttacggg aaggaatcag
      601 ggtggccaga tgtacagtgg cacgtctcat ggcggttatg ggacttgccg gtgttctccg
      661 gggtaaaaag gtccgtacga ccatcagccg gaaagccgtt gccgcaggcg accgcgtaaa
      721 ccgtcagttc gtggcagaac gacctgacca gctgtgggtg gctgatttta cttacgtcag
      781 cacatggcag ggcttcgtct atgtggcgtt tatcattgat gtgtttgccg gatacatcgt
      841 ggggtggcgg gtctcatcgt ctatggaaac gacattcgtg ctggatgcgc tggagcaggc
      901 gttgtgggcc cgtcgtccgt ctggcaccat ccatcacagc gataaaggct ctcagtatgt
      961 gtcactggcc tatacggagc gactaaaaga agccggatta ctggcatcaa cagggagtac
     1021 aggcgactcg tatgacaacg cgatggctga gagcatcaat ggtctttaca aagcggaggt
     1081 aatacaccgt aagagctgga aaaaccgtgc agaagtggaa ctggccacac taacgtgggt
     1141 ggactggtat aacaatcgac gattgctgg
//
LOCUS       DAZGLJ010000491         1160 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000491 DAZGLJ010000000
VERSION     DAZGLJ010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.504"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..117
                     /gene="ydfK"
                     /locus_tag="ACHK49_005237"
     CDS             <1..117
                     /gene="ydfK"
                     /locus_tag="ACHK49_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFO9265675.1"
                     /translation="LLDYIEGNIKKKSWLDNKELLQTAISVLKDNQNLNGKM"
     gene            434..1024
                     /locus_tag="ACHK49_005238"
     CDS             434..1024
                     /locus_tag="ACHK49_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO9265676.1"
                     /translation="MSRIFAYCRISTLDQTIENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT          386 a          237 c          222 g          315 t
ORIGIN      
        1 ttgcttgatt acattgaagg aaacataaag aaaaaatcat ggctggataa caaagaatta
       61 ttacaaacag cgatatcagt tcttaaagac aaccaaaatt taaatggtaa aatgtaatat
      121 aataaactta cttttttatc atttttccac tttaacaaca ttttgctcca cttttccacg
      181 accaaacaac ttgaaatctg gttaaaataa cacgcaacac tattcttctt ccttgagtcc
      241 gcccggaact cgaaaaacaa accgagttaa agccattttt cacaaaatcg attttgggtc
      301 tcaccaaaat tacggggttg catacgcatt cgtttatttt cgaacgtgta catacaaata
      361 tgcacaaaaa taatcaaaat tattttctga gatgcattat gatatgaaca ccaatttcgt
      421 atagagtctc actatgtctc gaatttttgc ttactgtcgg atatcaacgc tggatcagac
      481 catcgaaaat caacgccggg aaatcgaaag tgcaggtttt aaaatcaaac ctcagcaaat
      541 aatcgaagaa cacattagcg gctcagcagc aaccagtgag cgtcctggtt ttaaccggtt
      601 gcttgctcgc ctgaaatgtg gtgatcaatt gattgtgaca aaactggatc gccttggttg
      661 taatgcaatg gatatcagga aaacagtgga acaactgacc gaaacaggta tcagagtgca
      721 ttgcttagca ttggggggca ttgacctgac cagtccaaca ggaaaaatga tgatgcacgt
      781 aatttcagca gtcgctgaat ttgaacgaga ccttttactt gaacgcactc attccgggat
      841 agtaagagcc cgcggcgcag ggaaacgttt tggtcgacca cctgtgttaa atgaagaaca
      901 gaaacaggtg gtattcgaac gaattaagtc aggtgtaagt ataagtgcca ttgcccggga
      961 attcaaaacc tcgcggcaaa ccattttaag agccaaagca aaacttcaga cacctgacat
     1021 ataaaaaata atctcggtgt gagatgcttt acgtcttcca agcccccttc cttgccgtaa
     1081 atggaaagat acatctaatt atagaattta tatgttttac cctacggcag tgctggccat
     1141 tcaatatcct gtgcagttga
//
LOCUS       DAZGLJ010000492         1159 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000492 DAZGLJ010000000
VERSION     DAZGLJ010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.505"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..518)
                     /locus_tag="ACHK49_005239"
     CDS             complement(<1..518)
                     /locus_tag="ACHK49_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFO9265677.1"
                     /translation="MSFGQPCDEFPLSALPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQL"
BASE COUNT          361 a          269 c          216 g          313 t
ORIGIN      
        1 agctgattct taattgccgc agtcgtggca tcattaaaaa tgaatttata cctcacaggt
       61 tcttccgcac tgtttttctg aagtacttcc agttgtctga gggtttctga ctcatccgcg
      121 cctttgcgga gttctttatt cagttttgac aacaatgctt ttttctgagc ctttaaaagc
      181 tcttccttgt ttttccagac agccagttca cttttgtatc tggaatataa ctgcatctcc
      241 tgctggtaca ggggcttcat taataatttg tccactgcgg tttttcgttc tccggactcg
      301 gcaagcgtca ggaaaaaaag agagaccggc ccccgcagtg tattcagtct gcagacatca
      361 atcagattct ggcagacaag tgaaacagcg cccagggcag acgctgccac aagcccaagg
      421 ggagcctgcg taatttgctg agcttcaata acggcatcac gaatgagtgg aggcagcgca
      481 ctcaggggaa actcatcaca aggctgaccg aatgacatag taacctcact acacacagat
      541 ataaaagcac tgcaattctg gctattaata gcaaaaatag cagtcaaatc aatatgaggt
      601 aaaatttttt gttgatttaa tggttaaata ataaccacgg tgtattttgc tgctgaagaa
      661 agacaaaatg aatctgtgtg tcccctcctt cccttcggag cacatccact attcaaattt
      721 tacacaacca cgtacgaaca aaaagcatca tcccgggtat tgcagatacc ggtttcctgc
      781 cgggaagaat cataaacaga acaccgggaa taacgccttc aggaatcacc ccccctcctg
      841 aagaagaata aaataaagaa gactacagtt cctgcactga atgctgagta caatatttta
      901 tggacaggat gacattttta taaataacaa aaaccattta atactgatac atcaataaaa
      961 tacttcttcc ctgctgacac agataaaatg caccattttt tcttcaaata cttctgtata
     1021 atcgccagcc aatgatgaga atggattaaa ataacacttc ttcgacaaac acaaaaacag
     1081 ccttcatttt acatcatcat ccagtttcag ttaaccccgg acgagcgaca gtgtttcagt
     1141 acaggaatta aaagccagc
//
LOCUS       DAZGLJ010000493         1153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000493 DAZGLJ010000000
VERSION     DAZGLJ010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.506"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..863
                     /locus_tag="ACHK49_005240"
     CDS             <1..863
                     /locus_tag="ACHK49_005240"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO9265678.1"
                     /translation="KPVWALANDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSN
                     YGAALEKQGVEITLIYSGSHKVDGNPYSHLPDDVRETLQYRMDATRQMFAQKVSAYTG
                     LSVQAVLDTEAAVYSGQEAIDAGLADELVNSTDAITVMRDALDARKSRLSGGRMTKET
                     QSTTVSATASQADVTDVVPATEGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAH
                     GREEQARVLAETPGMTVETARRILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASD
                     AVNDLLNTPV"
     gene            873..>1153
                     /locus_tag="ACHK49_005241"
     CDS             873..>1153
                     /locus_tag="ACHK49_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO9265679.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAAS"
BASE COUNT          255 a          338 c          356 g          204 t
ORIGIN      
        1 taaaaccggt atgggcgctt gccaacgaca tgaactgcag tgcaggtcag ttgcttgcca
       61 gtgccgcctc ccggcgtctg gtcacgcaga ccgcccggac aggctccatc ggcgtcatga
      121 tggctcacag taattacggt gctgcgctgg agaaacaggg tgtggaaatc acgctgattt
      181 acagcggcag ccataaggtg gatggcaacc cctacagcca tcttccggat gacgtccggg
      241 agacactgca gtaccggatg gacgcaaccc gccagatgtt tgcgcagaag gtgtcggcat
      301 ataccggcct gtccgtgcag gctgtgctgg ataccgaggc tgcagtgtac agcggtcagg
      361 aggccattga tgccggactg gctgatgaac ttgttaacag caccgatgcg atcaccgtca
      421 tgcgtgatgc actggatgca cgtaaatccc gtctctcagg agggcgaatg accaaagaga
      481 ctcaatcaac aactgtttca gccactgctt cgcaggctga cgttactgac gtggtgccag
      541 cgacggaggg cgaaaacgcc agcgcggcgc agccggacgt gaacgcgcag atcaccgcag
      601 cggttgcggc agaaaacagc cgcattatgg ggatcctcaa ctgtgaggag gctcacggac
      661 gcgaagaaca ggcacgcgtg ctggcagaaa cccccggtat gaccgtggaa acggcccgcc
      721 gcattctggc cgcagcacca cagagtgcac aggcgcgcag tgacactgcg ctggatcgtc
      781 tgatgcaggg tgccccggca ccgctggctg caggtaaccc ggcatctgat gccgttaacg
      841 atttgctgaa cacaccagtg taagggatgt ttatgacgag caaagaaacc tttacccatt
      901 accagccgct gggcaacagt gacccggctc ataccgcaac cgcgcccggc ggattgagtg
      961 cgaaagcgcc tgcaatgacc ccgctgatgc tggacaccgc cacccgtaag ctggttgcgt
     1021 gggatggcac caccgacggt gctgccgttg gcattctggc ggttgctgct gaccagacca
     1081 gcaccacgct gacgttctac aagtccggca cgttccgtta tgaggatgtg ctctggccgg
     1141 aggctgccag cga
//
LOCUS       DAZGLJ010000494         1151 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000494 DAZGLJ010000000
VERSION     DAZGLJ010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.507"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..241
                     /locus_tag="ACHK49_005242"
     CDS             <1..241
                     /locus_tag="ACHK49_005242"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265680.1"
                     /translation="FLLSHATDFFSHCQKGALFTSISPLNKCKTCPVKRTTSCSQATN
                     HLQEQPSNIAETKTTKPSLSLMFGKFPETKKTKTK"
     gene            complement(300..>1151)
                     /locus_tag="ACHK49_005243"
     CDS             complement(300..>1151)
                     /locus_tag="ACHK49_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265681.1"
                     /translation="KKVREILKTPAWRTLETRIRRNGITFKSKAELDALSELYAIYSW
                     VSYCKRGDNYSVWFLNCPDVNINLHVCECRSSESDCYLPSRFIILPAPLRQKVKRKYS
                     ANSSGALGHNDSKSLYKTVLSVCGGKRETEMFFYSLPVKTNHHPYWIKSGVVKRFPPV
                     FGLLFYEQERLTGGVFRYVLIRFYNAFKENLFFVEIVSCCFLVEFFHDIDSFFINQRL
                     SKLGSFIDKWREGKEIWIKVYLQLIHTTLGITETEKLVNLYNSFFTDKVVLWDESVKK
                     VKWIYEK"
BASE COUNT          403 a          252 c          152 g          344 t
ORIGIN      
        1 gtttttactg tcacacgcta ctgacttttt cagtcattgc cagaaagggg ctctttttac
       61 ttccatttca ccactcaaca aatgcaaaac ttgtccagta aagcggacca cctcttgctc
      121 acaagcaacg aatcatctcc aggaacagcc ctcaaacatt gcagaaacaa aaacaacaaa
      181 accctcatta tcactgatgt tcggaaaatt tcccgaaaca aaaaaaacga aaacaaaatg
      241 agacgcatta tcatgaatgt agtcaaaaag acgttatggt ggctttcagt taaatggtac
      301 tatttctcat aaatccactt aacttttttt actgattcat cccagagaac cactttatca
      361 gtaaaaaatg aattatatag attgacgagt ttttctgttt cagttattcc caatgtagta
      421 tgtatcagtt gtagatatac ttttatccag atttctttac cctccctcca cttatctata
      481 aaacttccca gcttacttaa tcgttgattt ataaaaaacg aatcaatatc atgaaaaaac
      541 tcaacaagaa aacagcacga tacaatctca acaaaaaaca aattctcttt aaaagcatta
      601 taaaacctta tcaacacata acgaaaaaca cctcctgtta atctttcctg ttcataaaac
      661 aacaaaccaa aaacaggagg aaatctttta accacaccac ttttaatcca gtaaggatga
      721 tgattagtct ttactggcaa tgaataaaag aacatttcgg tttctctttt cccaccacaa
      781 acagacaaaa ctgtcttata aagacttttt gaatcattat gtcctagtgc cccagacgaa
      841 ttagcagagt attttctttt tactttttgc cttaatggag ctggaagaat aataaaacga
      901 gatggcagat aacaatcgct ttcagaagac cggcattcgc acacatgaag atttatattt
      961 acatccggac aattcaaaaa ccacacagaa taattatcgc cccgtttgca atatgaaacc
     1021 catgaataaa ttgcataaag ttcagaaagc gcatctaact ctgcttttga cttaaatgtg
     1081 attccgtttc ttcgaattct cgtttccagc gttctccatg ccggagtttt cagtatctca
     1141 cgaacttttt t
//
LOCUS       DAZGLJ010000495         1149 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000495 DAZGLJ010000000
VERSION     DAZGLJ010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.508"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..664
                     /locus_tag="ACHK49_005244"
     CDS             <1..664
                     /locus_tag="ACHK49_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO9265682.1"
                     /translation="ENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPI
                     GLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPDIAKQL
                     RLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQYPGMDTKTA
                     SDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQRTSRGI
                     RK"
     gene            661..>1149
                     /gene="ybfC"
                     /locus_tag="ACHK49_005245"
     CDS             661..>1149
                     /gene="ybfC"
                     /locus_tag="ACHK49_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO9265683.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMT"
BASE COUNT          369 a          226 c          262 g          292 t
ORIGIN      
        1 agagaacccg catcagctgc agcagcttat ccgcctgccg gggcagcagt atgatgagga
       61 gtccggcctg tattacaacc gccaccgcta ttatgacccg ctgcagggga ggtatatcac
      121 tcaggatccg attgggctga aggggggatg gaatttttat cagtatccgc tgaatccggt
      181 tcagtatata gattcaatgg gactggcatc aaaatatgga cacttaaata atggcggata
      241 tggagcgaga cccaacaaac cgcctacgcc cgatccaagt aaattgccgg acatagcgaa
      301 acaattaaga ctgccatatc ctattgacca ggccagtagt gcgcctaatg ttttcaaaac
      361 attcttcaga gcattaagcc cttacgacta cacactgtat tgcaggaagt gggtaaaacc
      421 aaatctgact tgtacgccac aggatgattc ccagtatcca gggatggata caaagacagc
      481 aagtgattac ctgccacaga caaattggcc aacaactcaa ttaccaccag gatatacttg
      541 tgcagaaccc tatttattcc cagacattaa taaacccgat gggccagcaa cagcagggat
      601 agatgatttg ggtgaaattt tagctaagat gaaacagaga acatcgagag gaataagaaa
      661 atgaaaagag ttttgttctt tttgctgatg atatttgtta gttttggtgt tatagctgat
      721 tgcgaaatac aagctaaaga tcatgattgt tttactattt tcgctaaggg tactattttt
      781 tccgcgtttc ctgtcttaaa taataaagcc atgtggagat ggtatcagaa tgaggacatc
      841 ggtgagtatt attggcaaac agaactgggt acatgtaaaa ataataaatt cacaccaagc
      901 ggggcgagac tcttaattcg cgtcgggtca ctacgcctta atgaaaatca tgccatcaaa
      961 ggaacgttgc aggagttgat aaatacagca gaaaaaacgg cgtttctcgg cgatcggttt
     1021 agaagttata taagagcggg tatctatcaa aaaaagagca gtgatccagt tcagttgttg
     1081 gcagtccttg ataattcaat catggtaaaa tattttaaag atgagaaacc aacttatgct
     1141 cgaatgaca
//
LOCUS       DAZGLJ010000496         1143 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000496 DAZGLJ010000000
VERSION     DAZGLJ010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.509"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..507
                     /locus_tag="ACHK49_005246"
     CDS             <1..507
                     /locus_tag="ACHK49_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265684.1"
                     /translation="AITANTGTLNNVTINENCVIRGKLSANQIEGDLVKTVGKAFPRN
                     NSYASGTVTVTVYDDQGFDRQIIIPPVLFRGTKHQNFNSPNQQSYWYSTCKLQVLKNG
                     VEIFHEPATDVSRVFSSVIDMPAGRGHVTLTFNVSSAGANNWTPTTYISDLLVVVMKK
                     STAGISIS"
     gene            576..>1143
                     /locus_tag="ACHK49_005247"
     CDS             576..>1143
                     /locus_tag="ACHK49_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO9265685.1"
                     /translation="MRHLYATILLFTTLLAGIAFPAQAESGHGAFSVGYAQVHPGGVP
                     ALSGTGARAGDLKGINVKYRYEFTDHLGGIVALSYASVKKSDTMKTGENTFHYESLRG
                     RYVSLMAGPVWQLSERVSLYGMAGMAYTRWSDSVQDYRRDEVKPGYVKETTTASDGHT
                     ARHLSPAWNAGIQFSPVETVVIDLAYEGS"
BASE COUNT          272 a          270 c          335 g          266 t
ORIGIN      
        1 gcaattaccg cgaataccgg cacgctcaat aatgtcacca ttaacgagaa ctgtgtcatc
       61 agagggaaac tgtctgcaaa ccagattgaa ggcgacctgg tgaagacggt ggggaaagcc
      121 tttccccgga ataacagtta tgccagcggg acggtaaccg tcacagttta cgatgaccag
      181 ggattcgacc ggcagattat cattcccccg gtgctgtttc gcgggacgaa acaccagaat
      241 ttcaacagcc cgaatcagca gtcgtactgg tattccacct gtaagctgca ggtgctgaag
      301 aacggggttg agattttcca tgaaccggca acggatgtca gccgggtgtt ctcatcggtg
      361 atagatatgc cggcagggcg gggtcatgtc accctgacgt ttaatgtgtc gtcggccggt
      421 gcgaacaact ggacgccgac aacgtacatc agtgatttac tggttgtggt catgaaaaaa
      481 tccacggcag ggatcagtat cagctgacgg tttattaacc cggacgggca cctcaggagg
      541 tgcctttttt attgactgaa aacaaagagg taatcatgcg gcatttatac gcaacgatat
      601 tattgtttac taccctgctg gcaggaattg cctttcctgc acaggctgaa agcggacacg
      661 gtgcattttc cgtgggatat gctcaggttc acccgggcgg cgtaccggca ttgtccggta
      721 ccggtgcgcg tgcaggtgat ttaaaaggga ttaatgtgaa ataccgttat gagttcacgg
      781 atcacctggg cggcattgtc gcgctgagtt atgcatcggt gaagaaaagt gacacgatga
      841 agacgggtga aaataccttc cattatgaaa gcctgcgcgg tcgttatgtc agtctgatgg
      901 ccggccctgt ctggcagctc agtgagcggg tcagtctcta tggcatggcc gggatggcgt
      961 acacccgctg gtctgacagt gttcaggatt accggcgtga tgaagtgaaa ccggggtatg
     1021 tgaaggagac caccaccgcc agtgatggcc atactgcgcg tcatctgtcg ccggcctgga
     1081 atgccgggat tcagttcagt cccgtagaga cggtggttat tgaccttgct tatgaaggtt
     1141 ccg
//
LOCUS       DAZGLJ010000497         1142 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000497 DAZGLJ010000000
VERSION     DAZGLJ010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.510"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..357)
                     /locus_tag="ACHK49_005248"
     CDS             complement(<1..357)
                     /locus_tag="ACHK49_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO9265686.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFMDALGLITSFSYANAEDDPKTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGV"
     gene            complement(425..>1142)
                     /locus_tag="ACHK49_005249"
     CDS             complement(425..>1142)
                     /locus_tag="ACHK49_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO9265687.1"
                     /translation="QVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPT
                     ITSGGNPPAFSLTPDGRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEG
                     DLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEKEHTDI
                     YSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNNWYPTA
                     SISDLLVVVMKKATAGITIS"
BASE COUNT          272 a          326 c          266 g          278 t
ORIGIN      
        1 cacacccgcc atcgcatacg cgctgaacca ttcattcacg cgtacagacg gccccgccat
       61 cacgctgaac cagcggttac gcacggaatc ttcatgccag cgggtatcgc tgtagcgcgt
      121 ttttgggtca tcctcagcat tggcataact gaaggacgta atcagcccca gcgcgtccat
      181 aaactcataa cggtatttca cgttaatccc gttcagatca tcactaccgg gaacgttcgt
      241 cgaggcatgg agataccccg cgctcagcgt ggactgatgt tctgctgcac tcgctggcgt
      301 agcagcggcg acctgccaga ctactgcgga caaaataaca gcacataatt tacgcataat
      361 tacctctcgc ttttctgcaa taaaaaaggc gccatttctg gcgcccgtat ctgggttata
      421 aaattcagct aatcgtgatg cctgcagtgg ctttcttcat caccacaacc agcaaatcgc
      481 tgatacttgc tgtgggatac cagttattta ccagccatgc tgacaccgaa aactccagtg
      541 tcatgtgacc gtgaccggca ggcatatcaa taacaccact gtaaatcagc gtattatcca
      601 gcgcggtacg gttataaatt tcagcaccgt ttttccgcac tatcagacgg catgaggagt
      661 aaatatcagt atgctctttc tcatgtttag cgccgctgaa tgccaccgcc ggaataacaa
      721 tctgccggtc aaacggctga tcgtcataaa ccctgacggt aatggttcct gatggccacc
      781 gctccggtgc acgggagtcc cgggggaaag ctttgcccac tgttttaacg agatcgcctt
      841 caatctggtt cgcggacaat tttcccagaa cccgacagtt ctcgttaatc gtgacgttgt
      901 tgagcgtccc ggagttcgca ttcacgttac cgctgatatc ggcatttttc gccgtcagcc
      961 gcccgtccgg tgtcagggaa aatgccggag gattaccgcc gctggtaatg gtgggagccg
     1021 tcagatattt caggaacact tcattcatga atatctgatc gccctgacca acaaacatcg
     1081 gctttgtgtt gccattcgca ggattaatca tcgcaatcct gtctgccgcc agcagcacct
     1141 ga
//
LOCUS       DAZGLJ010000498         1142 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000498 DAZGLJ010000000
VERSION     DAZGLJ010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.511"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..404)
                     /locus_tag="ACHK49_005250"
     CDS             complement(<1..404)
                     /locus_tag="ACHK49_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9265688.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISR"
     gene            complement(404..733)
                     /locus_tag="ACHK49_005251"
     CDS             complement(404..733)
                     /locus_tag="ACHK49_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9265689.1"
                     /translation="MKTFRWKVKPGMDVTSAPSVREVRFGDGYSQRVPAGLNADLKTY
                     SVTLSVSREEATALESFLAEHGGWKAFLWTPPYGYRQIKVTCAKWSSQVSMLRVEFSA
                     EFKQVVN"
     gene            complement(730..>1142)
                     /locus_tag="ACHK49_005252"
     CDS             complement(730..>1142)
                     /locus_tag="ACHK49_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9265690.1"
                     /translation="ASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIVHRGEFVFTKE
                     ATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFEQNNHVVINNDGTNGQIG
                     PAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
BASE COUNT          237 a          387 c          273 g          245 t
ORIGIN      
        1 cagcggctga tcacctcctg ctccggatcg gcgtcactgt ttccgttgac gaagttcacc
       61 gcatccagaa aacgggcgta aaccttacgc cggaccaccg ttccgccgac cagactctgc
      121 agatcttccg ccatcccggt gaccataccg tacaggttag aaaccgtcag cgtggggcgc
      181 gtactggtgc ctttgccatt cagttcaaaa ccgctcccct gaatgggata cggctgatac
      241 tgtcgcccct gccaggtgac cggctcacct ttttcgttct gctcattaca gaaaaaataa
      301 cgttcaccac cgacctctgt cagatcgatt tcccagagca ccacgctggc cgactgctcc
      361 gcacgggtgc attcattcag tgtttcctgc cggatatcct gcatcagttc accacctgtt
      421 taaactctgc gctgaactca acacgcaaca tactgacctg cgacgaccat tttgcgcagg
      481 tcacctttat ctgcctgtaa ccataaggcg gcgtccacag aaaggccttc cagcccccgt
      541 gctcagccag aaacgactcc agcgccgtgg cctcctcacg ggagacagac agcgtcacgc
      601 tgtacgtttt caggtcagcg ttcagcccgg caggcacacg ctgggaatag ccatcaccaa
      661 agcgcacctc cctgacggaa ggggccgatg tcacatccat accgggtttc actttccagc
      721 ggaaggtttt catcgtccac ctccggagaa caggccacca tcacgcatct gtgtctgaat
      781 ttcatcacgg gcacccttgc gggccatgtc atacaccgcc ttcagagcag ccggacctat
      841 ctgcccgttc gtgccgtcgt tgttaatcac cacatggtta ttctgctcaa acgtcccgga
      901 cgcctgcgac cggctgtctg ccatgctgcc cggtgtaccg acataaccgc cggtggcata
      961 gccgcgcatc agccggtaaa gattccccac gccaatccgg ctggttgcct ccttcgtgaa
     1021 gacaaactca ccacggtgaa caatccccgc tggctcatat ttgccgccgg ttcccgtaaa
     1081 tcctccggtt gcaaaatgga atttcgccgc agcggcctga atggctgtac cgcctgacgc
     1141 gg
//
LOCUS       DAZGLJ010000499         1135 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000499 DAZGLJ010000000
VERSION     DAZGLJ010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.512"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(25..189)
                     /gene="kil"
                     /locus_tag="ACHK49_005253"
     CDS             complement(25..189)
                     /gene="kil"
                     /locus_tag="ACHK49_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO9265691.1"
                     /translation="MDQTIMAIQTKFTIATFIGDEKMFREAVDAYKKWILIQKLRSSK
                     SIHYPPFLFS"
     gene            complement(158..322)
                     /locus_tag="ACHK49_005254"
     CDS             complement(158..322)
                     /locus_tag="ACHK49_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO9265692.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNNYGYPD"
     gene            complement(395..763)
                     /locus_tag="ACHK49_005255"
     CDS             complement(395..763)
                     /locus_tag="ACHK49_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFO9265693.1"
                     /translation="MSNIKKYIIDYDWKTSIEIEIDHDLMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPPMDGSEGIR
                     ITDIDTSGIFDPDDMTIKAA"
     gene            complement(913..>1135)
                     /locus_tag="ACHK49_005256"
     CDS             complement(913..>1135)
                     /locus_tag="ACHK49_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265694.1"
                     /translation="KVAKNAERSFAFPTKKLAIESLLRRKKYHLMRIKQDLAVVSTLV
                     DGMKNIDTSTPDIEYNFGHNQETENWVFY"
BASE COUNT          311 a          233 c          219 g          372 t
ORIGIN      
        1 atcgcccgcg aaatgccagg ctgattagga aaacaggaaa ggggggtagt gaatgctttt
       61 gcttgatctc agtttctgta ttaatatcca ttttttataa gcgtcgacgg cctcacgaaa
      121 catcttttca tcgccaataa aagtggcgat agtgaattta gtctggatag ccataattgt
      181 ttgatccatt tttcgggact cctggctgat taagtatgtc gataaggcgt ttccatccgt
      241 cacgtaattt acgggtaatt cgttcaagta aagattcgga agggcagcca gcaacaggcc
      301 accctgcaat ggcatattgc atggtgtgct ccttatttat acataacgaa aaacgcctcg
      361 agtgaagcgt aattggtatg cggtaacgcc gcgctcaggc ggctttgata gtcatatcat
      421 ctggatcaaa tattcctgat gtatcgatat cggtaattct tattccttcg ctaccatcca
      481 ttggaggcca tccttcctga ccatttccat cattccagtc gaactcacac acaacaccat
      541 atgcatttaa gtcgcttgaa attgctataa gcagagcatg ttgcgccagc atgattaata
      601 cagcatttaa tacagagccg tgtttattga gtcggtattc agagtctgac cagaaattat
      661 taatctggtg aagtttttcc tctgtcatta agtcatggtc gatttcaatt tctattgatg
      721 ttttccagtc gtaatcaatg atgtattttt tgatgtttga catctgttca tatcctcaca
      781 gaaaaaaatc gccctcacat tagagggcaa agaagatttc caataatcag aacaagtcgg
      841 ctcctgttta gttacgagcg acattgctcc gtgtattcac tcgttggaat gaatacacag
      901 tgcttactcg tactaataaa atacccaatt ttctgtttct tggttgtgtc caaagttata
      961 ttcaatatct ggtgttgatg tatcaatatt tttcatccca tcaacaagag ttgatacaac
     1021 agccaaatct tgttttattc tcattaaatg gtatttcttc cggcgcaata aactttcaat
     1081 agcaagtttc ttcgttggga atgcaaaaga tctttctgca ttttttgcta ctttc
//
LOCUS       DAZGLJ010000500         1133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000500 DAZGLJ010000000
VERSION     DAZGLJ010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.513"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1133
                     /locus_tag="ACHK49_005257"
     CDS             <1..>1133
                     /locus_tag="ACHK49_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO9265695.1"
                     /translation="QRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTGLKI
                     SGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLKAMA
                     DDGQLTADKVVPALISQLGVLRDEYAAMPETVSGSITKVENAFMAWVGGVNEASGVTK
                     TLSGVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVALAE
                     AQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARSAAQ
                     NALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALTIDE
                     IAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQE"
BASE COUNT          246 a          272 c          395 g          220 t
ORIGIN      
        1 cagcggacag gtaccgcttt ttcggataat gcggccctgt ttgcccgttc ggctgcctcg
       61 atgcgtgaat atggttacag tgctgatgat gtgctgaagg tgacggaggc catttccacg
      121 gggctgaaaa tctccggtgc cagtacggct gaggcgggtt cggtgatcac ccagttcagc
      181 caggcgctgg cacagggtgt gttgcgcggt gaggaattta attcggtcaa tgaaagtggt
      241 gaccggatca ttcgtgcact ggctgcaggc atgggcgtgg cccgtaaaga tctgaaggcg
      301 atggcggacg atggtcaact gacggcggat aaagtcgttc ctgcgttaat cagccagctg
      361 ggggtattgc gtgatgaata tgccgccatg ccagaaacgg tttccggtag tatcacgaag
      421 gtggaaaacg cctttatggc ctgggtgggc ggagtgaatg aggccagcgg ggtgacaaaa
      481 acgctctccg gcgtgctgaa cggtgttgcc ggacagattg ataatgtggc aacagctgtg
      541 ggtgcgctgg ttgccgtcgg ggttgcccgg tactttggca atatggcctc cggagcgatg
      601 tctgccacgg caggacttgt gacggctgca cgtaatgaag ttgcactggc ggaagcacag
      661 ttcaggggaa cgcagattgc cacagcgcgg gcaagggcag ccgtgtaccg tgctcagcag
      721 gccgtggcgg cagcccgcgg gacggagatg cagattgctg cagaggcccg tctggcggcc
      781 acacaggaac gcctgaacag aaatattgct gccagaagcg ccgcccagaa tgcgctgaac
      841 agtacaacgg cggtgggctc acgtctgatg agcggtgcgc tggggctggt tggtggcgta
      901 cccggactgg tgatgctggg ggctgcagca tggtacacgc tgtaccagaa tcaggagcag
      961 gccagggagt ctgcgcgcca gtatgcactg acgatagatg aaatcgcgca taaaacgccg
     1021 tcaatgtctt tgcctgaagc ctcagataat gaaggacgaa cacgggcggc gctgacagag
     1081 cagaaccggc tgattgatga acaggccagt cgggtgaaat ccctgcagga aaa
//
LOCUS       DAZGLJ010000501         1131 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000501 DAZGLJ010000000
VERSION     DAZGLJ010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.514"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(109..>1131)
                     /gene="ompC"
                     /locus_tag="ACHK49_005258"
     CDS             complement(109..>1131)
                     /gene="ompC"
                     /locus_tag="ACHK49_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO9265696.1"
                     /translation="AQAAEIYNKDSNKLDLYGKVNAKHYFSSNDADDGDTTYTRLGFK
                     GETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKFGDYGSIDYGRNYGVA
                     YDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGLVDGLNFAAQYQGKND
                     RTDVTQANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQVIYGNNSLNASGQNAEVWAA
                     GLKYDANNIYLATTYSETQNMTVFGKNHIANKAQNFEVVAQYQFDFGLRPSVAYLQSK
                     GKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINLIDKSDFTKASGVATDDIVAV
                     GMVYQF"
BASE COUNT          320 a          245 c          244 g          322 t
ORIGIN      
        1 gtttatatag ttagaagcaa ggttgtgctt ctaaaggaag tggcttgagg gagccactta
       61 tatgttgggg aggcaaagcc tcccacaaca tatgttttag taatcaaatt agaactggta
      121 aaccatacct acagcaacga tatcatcggt agcaacgcca gatgctttcg tgaaatcgct
      181 cttatcaatc aggttgattt tgtagtcaac aaaagtggac atatttttgt tgaagtaata
      241 ggttgcacct acatcaatat attcaaccag gtcctgatca ccccaagcac ccaagtcttt
      301 ccctttagat tgcagataag caacggacgg acgcagaccg aagtcgaact gatattgtgc
      361 aactacttcg aagttttgtg ctttgttggc aatatggttt ttaccaaaaa cagtcatgtt
      421 ttgggtttca gaataggtag tagccagata gatgttgttc gcatcatatt tcagaccagc
      481 tgcccatact tcagcatttt gaccagatgc attcaggctg ttgttaccgt agataacctg
      541 attattagtg cgatcagatt tagcataggt tgcacctacg ccgaaacctt catactcata
      601 agtagtggag aaaccgaaac catcaccatt agcttgagtt acgtcagtac ggtcattttt
      661 accctgatac tgagcagcaa agttcagacc atcaaccaga ccaaagaagt cattgttacg
      721 ataagttgca acacctgtgg tgcgaccagt catgaataca tctgtttggg tccaggtatc
      781 gccaccgaat tctggcagaa cgtcggtcca tgcaccaata tcgtatgcta caccgtagtt
      841 acggccataa tcgatggagc cgtagtcacc gaatttcagg ccagcgaagg caagacgggt
      901 tttatctttg gaggaacctt gagattcagc gcggttgcct ttgaattcat attcccactg
      961 accgaaacca gtcagttgat cgttgatttg ggtttcacct ttgaagccaa gacgggtata
     1021 agtagtatca ccatcatctg catcattaga ggagaagtag tgcttagcat taactttccc
     1081 gtacagatcc agcttgttac tgtctttatt ataaatttca gctgcctgag c
//
LOCUS       DAZGLJ010000502         1130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000502 DAZGLJ010000000
VERSION     DAZGLJ010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.515"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            177..1115
                     /gene="tdcA"
                     /locus_tag="ACHK49_005259"
     CDS             177..1115
                     /gene="tdcA"
                     /locus_tag="ACHK49_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO9265697.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAVV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT          315 a          200 c          255 g          360 t
ORIGIN      
        1 aaatcagggt ttatgctgat ttttatactt taacttgttg atatttaaag gtatttaatt
       61 tgcgtaacga taccatggaa agtattgaaa gttaatttgt gagtggtcgc acatatcctg
      121 ttcatttcat tttgatacac ttcatgccgt caatgaggta attaacgtag gtcgttatga
      181 gcactattct tcttccgaaa acgcagcacc tggtagtctt tcaggaagtc attagaagtg
      241 gctctatcgg ctcggctgca aaagaattag ggttaactca accggccgtc agtaaaatca
      301 ttaacgatat tgaagattat tttggtgtgg aattagtggt gcggaaaaat accggtgtaa
      361 cattaacacc tgccggtcaa ttgttactct cccgttccga atccattacc cgtgaaatga
      421 aaaatatggt taatgagata agcggtatgt cttctgaggc ggtggtggaa gtctcatttg
      481 gttttccttc attgattggt tttactttta tgtcagggat gatcaacaag ttcaaagagg
      541 tgttcccgaa agcgcaggtt tctatgtatg aagcgcaact gtcttcgttc ttaccggcaa
      601 tccgcgacgg tcgactggat tttgcgattg gtacgttaag tgcagaaatg aagcttcagg
      661 atttacatgt tgagccgctg ttcgagtccg agtttgtgct ggtagccagt aagtcccgaa
      721 catgcaccgg caccaccacg ctggagtcgt tgaagaacga acagtgggtg ttgccacaaa
      781 ccaatatggg gtactacagc gagcttctta ctacgttaca aagaaatggc atcagtattg
      841 aaaacatcgt taaaaccgac tcagtcgtga caatttataa tcttgttctc aatgctgatt
      901 tcttaactgt aattccttgt gatatgacgt caccttttgg ttcaaatcaa tttattacta
      961 ttccggttga agaaacatta cctgtggcac aatatgccgt ggtatggtcg aaaaattatc
     1021 gtattaaaaa agcagcatcg gttttggtgg aattagccaa agagtattca tcttataatg
     1081 ggtgtagacg aaggcaatta atagaagttg gttagttatt tgtttttatt
//
LOCUS       DAZGLJ010000503         1129 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000503 DAZGLJ010000000
VERSION     DAZGLJ010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.516"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            437..874
                     /locus_tag="ACHK49_005260"
     CDS             437..874
                     /locus_tag="ACHK49_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUKH-3 domain-containing protein"
                     /protein_id="HFO9265698.1"
                     /translation="MCELINEASKYGKVNERKMDIDLCCKKIEEEGYSVFVAFYDFIK
                     KYGGLYGFHSAYKDTTINEAFFIDPERASNRIFIERVNDYIERAGYNMIPVGECSNGH
                     ITLLYGENNVIYGGLDDYLWKYGDNLSSSIDILINGKEAVEIP"
     gene            894..1129
                     /locus_tag="ACHK49_005261"
                     /pseudo
     CDS             894..1129
                     /locus_tag="ACHK49_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529584.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
BASE COUNT          348 a          193 c          271 g          317 t
ORIGIN      
        1 atgattgtaa atgtttcaaa aactgaaaga ttggatggtt attttcgtgt gaatgatgat
       61 gaagataatt tattcttaga atggattgta aaagatggtt atttagagga aataattttg
      121 tctggttcat aaattcatct tctttgttga gaagattaac tgcctaggtg aaattatcct
      181 ctatgaccgg gacgctgcag gccacctggc agaagtcatc acgcaggaag acaagtcgct
      241 gtatcccttt gaaaaatccg gcaggctaat gggcatcttc agcccaggcg gcacatcaca
      301 gcgaatttga aggcaaatgc gaaaattagt gatcttgaag ttcatacagt attggtaaaa
      361 acaggcgaag cttttccaat gtgtaagaat tttcaaattt caatatttgg cgctgttgtt
      421 acaagcgggt gattatatgt gtgaattaat taatgaagca tcaaaatatg ggaaagtcaa
      481 tgaaagaaag atggacattg atttgtgctg taaaaaaata gaggaagaag gatattctgt
      541 tttcgttgcg ttctatgatt ttataaaaaa atatggcggt ttatatggct ttcattcagc
      601 ttacaaagac accactataa atgaggcttt ttttatagat ccagaaaggg cctctaatcg
      661 catttttata gaaagagtca atgattatat tgagagagct ggttacaata tgattcctgt
      721 tggcgagtgc agtaatggtc atatcacatt attatatggg gaaaataatg tgatatatgg
      781 cggcttggat gattatcttt ggaagtatgg ggataattta tcttcatcaa ttgatatatt
      841 gattaatggc aaggaggcag ttgaaattcc gtaaggccaa cttgatatga cacctggcac
      901 acaaccccga cgaccggatg tatatgctgg gcgggatgag tgacaccgcc agcaaccgca
      961 tcacgtttga gcgcgacagc cagtaccgga tcacgtgtgt cagtcacacc gacgggatcc
     1021 ggcttaaact gacgtaccat gccagcggct acctgaaagc cattcaccgc acggataacg
     1081 gcatacagac gctggcgacc tacgaacagg atgcgcgggg gcggctgac
//
LOCUS       DAZGLJ010000504         1117 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000504 DAZGLJ010000000
VERSION     DAZGLJ010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.517"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..769
                     /locus_tag="ACHK49_005262"
     CDS             <1..769
                     /locus_tag="ACHK49_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO9265699.1"
                     /translation="EPVIGIMGKSGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRL
                     SGHGHSMVITDLPGVGESRDRDAEYEALYRDILPELDLVLWLIKADDRALSVDEYFWR
                     HILQCGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPV
                     VAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRAQAREQFTGAVDRI
                     FDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFF"
BASE COUNT          245 a          303 c          302 g          267 t
ORIGIN      
        1 cgaacccgtg attggcatta tgggtaaaag cggggccggt aaatcctcac tctgtaatgc
       61 actgtttcag ggggaggtca ccccggtcag tgatgttcac gccggcaccc gggaagtacg
      121 gcgcttccgt ctgagtggcc acggtcacag catggttatc actgacctgc ccggggtggg
      181 cgagagccgg gacagggatg cagagtatga ggccctgtac cgtgacattc tgcctgaact
      241 tgacctggta ctgtggctga ttaaagccga tgaccgtgcc ctgtctgtgg atgagtattt
      301 ctggcgacac atcctgcagt gcggacatca gcaggtgctg tttgtggtga cgcaggccga
      361 caaaacggag ccctgccatg aatgggatat ggccggtatt cagccttctc ctgcacaggc
      421 acagaatatt cgcgaaaaaa cggaggcggt attccgtctg ttccggcccg tacatccggt
      481 tgtggccgta tcggcccgca ccggctggga actggatacg ctggtcagtg cgctcatgac
      541 agcgcttccc gaccatgccg ccagtcccct gatgacccga ctgcaggacg agctgcgcac
      601 ggaatctgtc cgcgctcagg cccgtgaaca gtttaccggt gcggtggacc ggatatttga
      661 cacggcggag agcgtctgtg ttgcctctgt tgtccgtacg gccctgcgcg ctgttcgtga
      721 cacggtggtc tctgttgccc gtgcggtatg gaactggatt ttcttctgaa cctgtcgtga
      781 ctgatgccct ccctgactct gagtctgctc acaaaagcac tgttttcgtt actgtctctc
      841 ttgtccgtgc aatagctcaa taatagaata aaacgatcaa tatctatttt atcgatcgtt
      901 tatatcgatc gataagctaa taataacctt tgtcagtaac atgcacagat acgtacagaa
      961 agacattcag ggaacaacag aaccacaatt cagaaactcc cacagccgga cctccggcac
     1021 tgtaaccctt tacctgccgg tatccacgtt tgtgggtacc ggctttttta ttcaccctca
     1081 atctaaggaa aagctgatga aacgacatct gaatacc
//
LOCUS       DAZGLJ010000505         1114 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000505 DAZGLJ010000000
VERSION     DAZGLJ010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.518"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..533
                     /locus_tag="ACHK49_005263"
     CDS             <1..533
                     /locus_tag="ACHK49_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO9265700.1"
                     /translation="INGLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFKVQFPSG
                     KGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSYVVPLAFVKNLEKTLTVNTGAL
                     LTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTFSKANTQSGDKGAYTCEVTDSAEQPQ
                     SITSDACTVTVNGAGG"
     gene            539..913
                     /locus_tag="ACHK49_005264"
     CDS             539..913
                     /locus_tag="ACHK49_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO9265701.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWRNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            937..>1114
                     /locus_tag="ACHK49_005265"
     CDS             937..>1114
                     /locus_tag="ACHK49_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO9265702.1"
                     /translation="MRLALRLGRTLSELRHSLSASEAMMWMEFDRVSPLGDERGDIRN
                     AQIVKAVFGAQGMNV"
BASE COUNT          249 a          257 c          362 g          246 t
ORIGIN      
        1 acatcaacgg tctgggggcg tcgtccgaga tttccatgtc gggtaatttt tatctgaatc
       61 aggcccagaa cgccctgcgt gatgcctatg acaatgacac ggtgtatgcg tttaaggtgc
      121 agtttccgtc cggtaagggc tttaagttcc tggcggaagt gcgtcagcac acctggtcat
      181 ccggtaccaa cggcgtggtg gctgcaacgt tttcacttcg cctgaagggt aaaccggtgt
      241 cctatgtggt accgctggcg tttgtgaaaa atctggagaa gacacttacc gtgaataccg
      301 gtgcgctgct gacaatgtca gtcagtgtca acgggggaac gccgccttat aaacacgcct
      361 ggaagaagga tggtcagccg gtagagggac agactactga cactttcagt aaagccaata
      421 cgcagtcagg tgataagggg gcttatacct gcgaggtaac ggattctgca gaacagccgc
      481 agagcattac ctctgatgcg tgtacagtaa cggttaatgg tgcgggcgga taaggcttat
      541 ggcaaaagat ctgaaaacac tggcgctggc cagactgtcg gggttccgtc ataaaacggt
      601 gaaggtgccg gaatggagaa atgtcagcgt ggtgctgcgg gagccttcgg cagaggcctg
      661 gtatctgtgg caggaagtgc tcaatggtga tggagaggat gacgataccc tgtcggtggt
      721 ggcgaaaacc cgccgtaacc tggaagcgga tgtgacgctg ttctgcgatg tcctgtgtga
      781 tactgacctg caacgggtgt tcactccgga cgaccgtgag caggtgctgg ccgtctatgg
      841 tccggtacat gcccggttgc tgcgtcaggc actggaactg atcgctgatg cagagtcggc
      901 cagaaaaaag tagcccgccc ggaaattcgc tttctgatgc gacttgcgct ccgtctgggg
      961 cgcaccttat ccgaactgcg gcacagcctg agtgcgagcg aggcgatgat gtggatggag
     1021 ttcgacaggg tatccccgct gggtgatgag cgcggggata ttcgtaatgc acagatcgtg
     1081 aaagcggttt tcggggcaca ggggatgaat gttg
//
LOCUS       DAZGLJ010000506         1103 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000506 DAZGLJ010000000
VERSION     DAZGLJ010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.519"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1103
                     /gene="rhsB"
                     /locus_tag="ACHK49_005266"
     CDS             <1..>1103
                     /gene="rhsB"
                     /locus_tag="ACHK49_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO9265703.1"
                     /translation="VYDRSNTQVRSFTYDDKYRGRMVAHRHTSRPEIRYRYDSDGRVT
                     EQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEAGLKRVVKKEHADGSVTQSQFD
                     AVGRLRTQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSATGPDGLE
                     IRREYDELGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYAQLLSF
                     TDCSGYVTRYDHDRFGQMTAVHREEGLSQYHAYDSRGQLIAVKDTQGHETRYEYNIAG
                     DLTAVIAPDGSRNGTQYDAWGKAVRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRY
                     DVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDE"
BASE COUNT          281 a          323 c          328 g          171 t
ORIGIN      
        1 agtgtatgac cgcagcaata cacaggtgcg cagctttact tacgatgata aataccgggg
       61 ccggatggtg gcgcaccgtc acacgagccg accggaaatc cgttaccgtt acgacagcga
      121 cgggcgggtg acagaacagc taaacccggc aggcttaagc tacacgtatc agtatgagaa
      181 agaccacatc accatcaccg acagcctgga ccgccgtgaa gtgctgcaca cgcagggcga
      241 agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca cgcagagtca
      301 gtttgacgcc gtgggcaggc tcaggacaca gacggatgcc gcaggccgga caacagaata
      361 cagcccggat gtggtgacgg gcctcatcac gcgcatcacc acgccggatg gcagggcatc
      421 ggcgttttac tataaccacc acagccagtt aacgtcagcc accgggcctg acgggctgga
      481 aatacgccgg gaatatgatg aattgggccg tctgattcag gaaactgccc ctgacggcga
      541 tatcacccgc taccgttatg ataatccaca cagtgactta ccctgcgcaa cggaagatgc
      601 caccggcagc cggaaaacca tgacgtggag ccgttacgct cagttgctga gcttcaccga
      661 ctgttccggt tatgtaaccc gttatgacca tgaccgcttc gggcagatga cggcggtgca
      721 ccgcgaggaa ggcctgagcc agtaccacgc atacgacagc cgtggacagt taattgccgt
      781 gaaagacacg cagggccatg aaacgcggta tgaatacaac atcgccggtg acctgaccgc
      841 cgtcattgcc ccggacggca gcagaaacgg gacacagtac gatgcgtggg gaaaggccgt
      901 ccgtaccacg cagggcgggc tgacgcgcag tatggaatac gatgctgccg gacgggtcat
      961 ccgcctgacc agtgaaaacg gcagccacac caccttccgt tacgatgtac tcgaccggct
     1021 gatacaggaa accggctttg acggccgcac acagcgttat caccacgacc tgaccggcaa
     1081 acttatccgc agcgaggatg agg
//
LOCUS       DAZGLJ010000507         1102 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000507 DAZGLJ010000000
VERSION     DAZGLJ010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.520"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1102
                     /locus_tag="ACHK49_005267"
     CDS             <1..>1102
                     /locus_tag="ACHK49_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9265704.1"
                     /translation="KSTQMMSVIDAIGEGPIEGPVKGLQSILVNKTPLTDTDGNPVIH
                     GVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTRTITSANIDRLRVTFGVQS
                     LLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTTSQYLASVILENLPERPFN
                     IRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQMTVNY
                     HIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAA
                     DVDKWALYAIGQYCDQRVPDGFGGTEPRMTFNAYLSQQRKAWDVLSDFCSAMRCMPVW
                     NGQTLTFVQDRPSDVVWPYTSSDVVVDDNGVG"
BASE COUNT          245 a          288 c          364 g          205 t
ORIGIN      
        1 ttaagtccac gcagatgatg agcgtgattg atgcgattgg tgaagggccg attgaaggtc
       61 cggtgaaggg gctgcagagt atcctggtga acaaaacccc gctgacggac acggacggta
      121 atcctgtgat acatggtgtg acagcggtct ggcgcgccgg ggagcaggag cagacaccac
      181 ctgaaggctt tgagtcctcc ggggcggaaa ccgcactggg cgtggaagtg acgaaagcaa
      241 agccggtgac gcgcaccatt acgtccgcga acattgaccg cctgcgggtc accttcgggg
      301 tgcagtcact gttggagacc acctcaaagg gcgaccgtaa tccctcttct gtccgactgc
      361 tgattcagtt gcagcgtaac ggtaactggg tgacggaaaa ggatgtcacc attaacggca
      421 agaccacctc acagtacctg gcgtcggtga ttctggagaa tctgcctgag cggcccttta
      481 acatccggat ggtccgggag acggcggaca gcaccacgga ccagctgcag aacagaacgc
      541 tgtggtcgtc atacaccgaa atcatcgatg tgaaacagtg ctacccgaac acggccattg
      601 tggggctgca ggtggatgcg gagcagttcg gcggtcagca gatgacggtg aactaccata
      661 tccgcggtcg catcatccag gtgccgtcaa actacgaccc ggaaaaacgc acttacagcg
      721 gtatctggga cggcagtctg aaaccggcat acagcaacaa cccggcctgg tgtctgtggg
      781 acatgctgac ccacccgcgc tacgggatgg ggaaacgcct gggggccgcg gacgtggaca
      841 aatgggcgct gtatgccatc gggcagtact gtgaccagcg tgttccggac ggcttcggag
      901 ggacagagcc gcggatgacg tttaatgcgt acctgtcaca gcagcgtaag gcgtgggacg
      961 ttctcagtga tttctgctcg gcgatgcgct gtatgccggt atggaacggc cagacgctga
     1021 cgttcgttca ggaccgtccg tcggatgtgg tgtggccgta caccagcagc gatgtggtgg
     1081 tggatgataa cggcgtgggg tt
//
LOCUS       DAZGLJ010000508         1089 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000508 DAZGLJ010000000
VERSION     DAZGLJ010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.521"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..876
                     /locus_tag="ACHK49_005268"
     CDS             <1..876
                     /locus_tag="ACHK49_005268"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO9265705.1"
                     /translation="KITPNPETLDKNFLYHFLQKELDKIHATTDVVTVKHLSVKKIQD
                     IKIRLPSLKEQKRIAAILDKADAIRQKREQAIKLADDFLRAKFLEMFGTPANNIHRFP
                     KGTIRDLVDSVNYGTSAKASIDSGEYPILRMGNITYQGRWDFTDLKYLDLSVKEKDKY
                     LVKEGDLLFNRTNSKELVGKTAVYEEDRPMAFAGYLIRVRPNSIGNNYYISGYLNSIH
                     GKITLMNMCKSIVGMANINAQELQNIEILIPPKHLQDEYEIIYKKIKKGLSIYDKSAM
                     QLQLLASNLSNKYFM"
     gene            885..>1089
                     /locus_tag="ACHK49_005269"
     CDS             885..>1089
                     /locus_tag="ACHK49_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9265706.1"
                     /translation="MKLTKLVLENFRSVRERQEIDFAPVTLLLGPNSAGKTTILIALF
                     YLQQILDREQCDPIYLEAMGNRRI"
BASE COUNT          389 a          171 c          200 g          329 t
ORIGIN      
        1 aaaattacgc caaatcctga aacacttgat aaaaattttc tatatcattt cttgcaaaag
       61 gaattggata aaattcatgc tacaacagat gttgtgactg taaaacattt gtctgtaaaa
      121 aagatacaag atattaaaat taggcttcct tcattaaaag aacaaaaacg cattgctgct
      181 attcttgata aagctgatgc tatacgccaa aagcgcgaac aagccatcaa actggctgat
      241 gattttttgc gggctaagtt tctagagatg tttggaactc ctgcaaacaa catacatcgt
      301 ttcccgaaag gaacgattcg cgacttggtt gattcagtaa attatggaac ttcagcaaaa
      361 gcctctattg attctggtga atatcctata ttacggatgg gtaatataac atatcaaggt
      421 aggtgggatt ttacagattt aaaatatctt gatctcagtg taaaagaaaa ggataaatat
      481 ttggttaaag aaggcgatct tcttttcaat cgtactaaca gtaaggaact tgttggtaaa
      541 acagctgtgt atgaagaaga caggcctatg gcatttgctg gatatttaat ccgcgttaga
      601 cctaattcta tcggaaataa ctactatatt tcagggtatc ttaattccat ccatggcaaa
      661 atcacattga tgaatatgtg taaaagtatt gttggcatgg ctaatattaa tgcacaggaa
      721 ttgcagaata tagagatatt aatcccacct aagcatctac aagatgagta tgagattatt
      781 tataaaaaaa taaaaaaagg actaagcatt tatgataaaa gcgcaatgca acttcaactt
      841 ttagcatcaa acctatcgaa taaatatttc atgtaaggtt cattatgaaa ttaactaaac
      901 ttgtacttga aaatttcaga tctgttagag agcggcagga aattgatttt gcacctgtaa
      961 cattactatt ggggccaaac agcgcgggca agactactat tttaattgca ttattttatc
     1021 ttcaacaaat attagaccgt gagcagtgtg atcctatata tttagaagct atggggaaca
     1081 ggcgcatag
//
LOCUS       DAZGLJ010000509         1089 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000509 DAZGLJ010000000
VERSION     DAZGLJ010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.522"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>1089)
                     /locus_tag="ACHK49_005270"
     CDS             complement(<1..>1089)
                     /locus_tag="ACHK49_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9265707.1"
                     /translation="TGGRILSIDAASRTLTLDREVTLPETGTSTVNLINGSGKPVSVD
                     ITAHPAPDRIQVSTLPDGVATYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHV
                     PEKEAIVDNGARFEPMSGSLNSVIPPAVQHLTAEVTADSGEYQVLARWDTPKVVKGVS
                     FLLRLTVTADDGSERLVSTARTTETTYRFRQLAPGNYRLTVRAVNAWGQQGDPASVSF
                     RIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRITDIRQVETTARYLG
                     TGLYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGRASDDAEGYLDFFKGKITES
                     HLGKELLEKVDLTEDNTSRLDEFSKEWK"
BASE COUNT          207 a          346 c          299 g          237 t
ORIGIN      
        1 ccttccactc tttcgaaaac tcatccagtc tgctggtgtt atcctccgtc agatcgactt
       61 tttccagcag ctccttgccg agatgagatt cggttatctt gcctttgaaa aaatccagat
      121 aaccttccgc atcatcgctc gcccgaccga cggcctccac gaatgccgat ttgccaacag
      181 tgttcacact gcggatataa aagtaataat catggcccgg tttgatattg atactggcgg
      241 ctatccagta cagccccgtg ccaaggtagc gggctgtggt ttcaacctgc ctgatatcgg
      301 taatccgctt ttccgagaac cagaactcaa actgtaccgt cgggtcataa acggcaagat
      361 gcggcgtggc ggttatctga aaatagcccg gcgtcagctc aatcctcgac ggtgctgccg
      421 gtgcggcaat ccggaacgat accgacgccg gatcaccctg ctgcccccac gcatttaccg
      481 cccggactgt cagcctgtag ttccccggcg ccagttgcct gaagcggtat gtggtttccg
      541 tcgtccgggc cgtgctgacc agccgctcac tgccgtcatc cgctgttacg gtcagacgga
      601 gcaggaagct cacgcccttc accaccttcg gcgtgtccca gcgcgccagc acctgatatt
      661 ccccgctgtc tgcggtgact tcggcagtca ggtgctgcac tgccggcggg atgacgctgt
      721 tcagtgagcc ggacatcggc tcaaagcggg ccccgttatc cacaatggct tctttttccg
      781 gcacatgctg caccgccgtg atggcaaagg tgccgtccgt gttttcccgg atggaaacac
      841 agcggaacag gcgacgacgc agtgacggca gggagagtcc ccacacaccg tatgtcgcca
      901 cgccatccgg cagggtactg acctgtatcc ggtccggcgc ggggtgtgcg gtgatgtcca
      961 cactcaccgg cttaccgctg ccgttaatca ggttcaccgt cgatgtacct gtctccggca
     1021 gtgtcacctc acggtccagc gtcagggtgc ggctggcggc atcgatggac aggatacgtc
     1081 cgccggtca
//
LOCUS       DAZGLJ010000510         1077 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000510 DAZGLJ010000000
VERSION     DAZGLJ010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.523"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>1077)
                     /locus_tag="ACHK49_005271"
     CDS             complement(<1..>1077)
                     /locus_tag="ACHK49_005271"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO9265708.1"
                     /translation="SLPVFLISALNFVFTPFSFRYIFKPFFCILFICSSIVTYATMKY
                     GVQFDKTMMQNIFETNAGEMTSYFNMSVVLWFLFTGILPCGLLLLVNIRYPETWIKGI
                     IYRLISMFASLLIIFAIAFFFYKDYASVGRNNSSLNKEIIPTNYIYSGFKYVRDFFVS
                     PGEFRQTGTDASRTINEKQKPVIMFLVVGETARSQNYALNGYSRGTNDFTKKYNELIS
                     FHNVQSCGTSTAISVPCMFSDMKRKEFNSRKAVNSENVLDILYRTGVNLLWIENDGGC
                     KGVCKRIPTINIEPSNSDNTLCKKNSCYDEVMLKNIDEYINNNSEDKLIVFHLMGSHG
                     PTYYLRYPEPHKYFKPTCDRSDIEN"
BASE COUNT          374 a          197 c          166 g          340 t
ORIGIN      
        1 attttcgata tcactacgat cacatgttgg tttaaaatat ttatgtggct caggataacg
       61 gagatagtag gtaggaccat gactacccat caaatgaaat acgataagtt tatcctctga
      121 gttattattt atatactcat caatattttt tagcataacc tcatcataac atgagttttt
      181 cttgcataat gtattatctg aatttgatgg ctcaatatta attgtaggga ttcgcttaca
      241 tacaccttta catcccccgt cattttcaat ccagagtaaa tttacccccg ttctgtacag
      301 aatatccagt acattttccg aatttaccgc tttgcgggaa ttgaactctt ttcgcttcat
      361 atctgaaaac atgcatggaa cagatatggc tgtggatgtg ccacacgact gcacattatg
      421 aaaggatata agttcattat atttctttgt aaaatcattg gtaccacgag aatatccatt
      481 cagagcatag ttttgacttc ttgctgtctc cccgacaact aaaaacataa ttaccggctt
      541 ttgtttttca tttattgtgc gggatgcatc cgttccagtc tgacgaaact cgccaggaga
      601 aacaaaaaaa tcccttacat atttaaaccc actgtagata taattagtag gtattatttc
      661 tttatttaat gaagagttgt ttctgccaac tgatgcataa tccttataaa agaaaaacgc
      721 aatcgcaaat ataatcaaga gagatgcaaa cattgatatt aaacgatata taataccctt
      781 tatccatgtc tcaggataac ggatatttac taacaacaat agtccacatg gaagtatacc
      841 cgtaaataaa aaccacagta caacagacat attgaaatat gatgtcattt cacctgcatt
      901 tgtttcaaaa atattctgca tcattgtttt atcaaactgc acaccatatt tcatggtggc
      961 atatgtaaca atggaactac agataaacag aatacaaaaa aaaggtttaa atatatacct
     1021 gaatgagaac ggtgtaaaaa caaaatttaa tgcagatatc agaaagactg gaagaga
//
LOCUS       DAZGLJ010000511         1069 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000511 DAZGLJ010000000
VERSION     DAZGLJ010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.524"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..663
                     /locus_tag="ACHK49_005272"
     CDS             <1..663
                     /locus_tag="ACHK49_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO9265709.1"
                     /translation="DGRDYDPDVLQKAVNDAVANIPQPADGKSLTPDDVRPMLEQMVK
                     EAVSHIHVPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPRG
                     TYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTF
                     SLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHSSAWTLAAKRGRDA
                     GG"
     gene            666..992
                     /locus_tag="ACHK49_005273"
     CDS             666..992
                     /locus_tag="ACHK49_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO9265710.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            1002..>1069
                     /locus_tag="ACHK49_005274"
     CDS             1002..>1069
                     /locus_tag="ACHK49_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265711.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNA"
BASE COUNT          233 a          258 c          357 g          221 t
ORIGIN      
        1 gacggtcgtg actatgatcc cgatgttctg cagaaggcgg tgaatgatgc ggtcgcaaat
       61 attccgcagc cggcagacgg taaaagtctc accccggatg atgtgcgtcc gatgcttgaa
      121 cagatggtga aggaggcggt aagccatatt catgttccgc gtgatggtcg tgactacgat
      181 ccggatgttc tgcagaaggc ggttctggat gcggtgagtg ccctgccggc tccgcaggac
      241 gggcgtgatg ccacggctct ggaaatactc cccgccattg acgatcaaaa atcctttccc
      301 cggggcacgt atgccacaca ccagggcgga ctctggcggg cgtatgaaaa aacgcacggg
      361 atgcggggat gggaatgcct ggttgacggg gtggcggata ttgacgtcag catgacgggt
      421 gagcggttgt tctctgtggt ggtccggcag agcagtggcc agcgtacgga aaaaacattt
      481 tccctgccgg tgatgctcta ccgcggtgtg ttcagagccg gtgaaaccta ccaccccggc
      541 gatacggtga cgtggggggg ctcgctgtgg cactgcaaca gtatgaccga agataaaccc
      601 ggagaagctc attcatcagc ctggaccctg gctgcaaaac gtgggcggga tgcaggaggc
      661 tgaaaatgac ggcattactg acactggaag agatcaaggc acatctgcgt gtcgaccatg
      721 acgcggatga tgacatgctg atggacaagg ttcgtcaggc taccgccgtg ctgctggcct
      781 acattcaggg cagccgggat aaagtgatcc gtgaggacgg tgaactgatc ccgggcgagg
      841 cattaacccg gatgaagggg gctgccatgc gactgaccgg gatgctgtac cggaatccgg
      901 atcttgcgga gcgggaagaa ctgcttcagg gggagctgcc gttttctgtt tccgtgctga
      961 tttacgattt gcgttgtccg acggtgttat gaggaggggg aatggcaata tctgcaggtc
     1021 gtctgacaca gatgataagt gttctgaacc cggtgttaac ccgtaacgc
//
LOCUS       DAZGLJ010000512         1063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000512 DAZGLJ010000000
VERSION     DAZGLJ010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.525"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1063
                     /locus_tag="ACHK49_005275"
     CDS             <1..>1063
                     /locus_tag="ACHK49_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO9265712.1"
                     /translation="AFIEVPRKNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARI
                     VFDDARQMCLLSRPLRRRVNIQAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEY
                     HLHPDNGVYSALELGMGARPEGLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSI
                     FVLIYELDDENEVDDPAMWIKANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFN
                     IWCQGTTPWMGNGAWAECAGTFTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIM
                     LVSRHYLPEFQLQNPANKNRAIYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNI
                     RLVGFDTWNATHLRTQLQGA"
BASE COUNT          271 a          263 c          295 g          234 t
ORIGIN      
        1 gcgcctttat cgaagtgccg cgcaaaaatg cgaaatcaac gaccgccgcg attctggcta
       61 actggtttct gattatggag aacggtcagc aggatattta caccgcagcg gtgagccgtg
      121 accaggcgcg gatcgtcttt gatgatgcgc gtcagatgtg ccttttatcc cgaccgttac
      181 gcaggcgggt gaatattcag gcgcataagg tgatacaccc gaaaagcaac agcctgttaa
      241 agccgctggc agcaaaagcg gcaaccatcg agggcacaaa ccccagcctt tccatcgtgg
      301 acgaatacca cctacacccc gataacggcg tttattctgc gcttgagctg gggatgggcg
      361 cacgtcccga agggctgtta tttgccatta ccacatcagg cagtaacgtc gtttcagcct
      421 gtaagcagca ctatgattat tgctgccaga tcctggacgg cgaagaggtc aacgattcaa
      481 tttttgtact gatttacgaa ctggacgacg aaaacgaggt tgatgatccg gcgatgtgga
      541 taaaagcgaa ccctaatatt gatgtttccg tggatcgtga aaaactggcc tcaaccatcc
      601 agaaagcgcg gggtattccg tcgcagtggg tggagatgat gacaaagcga tttaatatct
      661 ggtgtcaggg gactaccccg tggatgggta acggcgcatg ggctgagtgt gccggaacgt
      721 tcacggagga agatttacac ggccaggagt gttacgcggg gctggactta tcatcaacca
      781 gcgatatttc cagcgtgtgc tatgcctttc cggtcggtaa aaccattatg ctggtttccc
      841 gtcactatct gccggaattt cagctacaga accccgccaa taaaaaccgc gccatctatc
      901 gtcagtgggc taaagcaggc tggatacgca caacgcctgg cgactgcatt gattacgacc
      961 ggatcagaga tgacatcatg caggacgcgg agaaatttaa tatcaggctg gtgggctttg
     1021 atacgtggaa cgccacgcat ctcagaacgc aattacaggg ggc
//
LOCUS       DAZGLJ010000513         1061 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000513 DAZGLJ010000000
VERSION     DAZGLJ010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.526"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..181
                     /locus_tag="ACHK49_005276"
     CDS             <1..181
                     /locus_tag="ACHK49_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265713.1"
                     /translation="LREQKMKINHRGFCFYWPEKTVTPGNDICHGAYRKCEEIQKFYS
                     VMTQAPSGVPVVFGA"
     gene            217..>1061
                     /locus_tag="ACHK49_005277"
     CDS             217..>1061
                     /locus_tag="ACHK49_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9265714.1"
                     /translation="MGKGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGSETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVQTTSQGDRNPASVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLT"
BASE COUNT          269 a          273 c          325 g          194 t
ORIGIN      
        1 gctgcgggaa cagaaaatga agattaacca cagggggttt tgtttttatt ggcccgaaaa
       61 aactgtaacg cccgggaatg atatctgcca cggggcgtac agaaaatgtg aagaaattca
      121 gaaattttat tccgtcatga cacaggcacc ctccggggtg cctgtcgttt ttggggcata
      181 aacagattca gacatcagac aggagagggg gacagagtgg gtaaaggtgg cggcagggcg
      241 cacacgccgg tagaggcaaa ggacaatctt aagtccacgc agatgatgag cgtgattgac
      301 gccattggtg aagggccgat tgaaggtccg gtgaaggggc tgcagagtat tctggtgaac
      361 aaaaccccgc tgacggacac ggacggtaat cctgtgatac atggtgtgac agcggtctgg
      421 cgcgccgggg agcaggagca gacaccgccg gaaggttttg agtcatccgg ctctgaaacc
      481 gcactgggcg tggaagtgac gaaggcaaag ccggtgacgc gcaccattac gtccgcgaac
      541 attgaccgcc tgcgggtcac cttcggggtg cagtcactgg tgcagaccac ctcacagggt
      601 gaccgtaacc cggcatccgt ccgcctgctg attcagctgc agcgtaacgg taactgggtg
      661 acggaaaagg atgtcaccat taacggcaag accacctcac agttcctcgc ttcggtgatt
      721 ctggataatc tgcctccccg cccctttaac atccggatgg tcagggagac ggcggacagc
      781 accacggacc agctgcagaa taagacgctc tggtcgtcat acaccgaaat catcgatgtg
      841 aaacagtgct acccgaacac ggcgattgtg gggctgcagg tggatgcgga gcagtttggc
      901 ggtcagcaga tgacggtgaa ctaccatatc cgcggtcgca tcatccaggt accgtcaaac
      961 tatgacccgg aaaaacgcac gtacagcggc atctgggacg gcagcctgaa accggcatac
     1021 agcaacaacc cggcctggtg cctgtgggac atgctgactc a
//
LOCUS       DAZGLJ010000514         1058 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000514 DAZGLJ010000000
VERSION     DAZGLJ010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.527"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1058
                     /locus_tag="ACHK49_005278"
     CDS             <1..>1058
                     /locus_tag="ACHK49_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO9265715.1"
                     /translation="EVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHK
                     DPVALNKYICEYLTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEA
                     EPSVEREGPFYFLFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGL
                     PQNVDTAEDSEQPEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDP
                     KWVKGIQTRDSVNQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKACTAC
                     GQTGGGNCPDCGAVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHN
                     SDNETGETADHPIKVNGH"
BASE COUNT          346 a          250 c          270 g          192 t
ORIGIN      
        1 gaagttatcg acaacgaccg cgaagactgg aagcgtatct cggcatcaat gcgcaaacag
       61 cctgatgccc ttcgctacga ccgccaaact atttttggcc ttgtccgtga gcgtccgatc
      121 gacattcaca aagatcccgt agcactgaac aaatatatct gcgaatacct gacgacaaag
      181 ggcgtgtttg agaatgaaga aacagacctg ggcactgttg atgttctcca gtcatcagaa
      241 acacaaactg atgcagtgga aactgaggta tctgatatcc caaaaaatga aaccgcgccg
      301 gaagctgaac catctgtaga gcgtgagggg ccgttctatt tcctcttcgc agataaggac
      361 ggagaaaaat acggtcgcgc aaacaaactt tctggtctgg ataaggcact ggctgctggc
      421 gccactgaaa tcacaaaaga agaatatttt gcccgaaaaa atggcacata cacgggctta
      481 ccgcaaaatg tagataccgc tgaagattca gaacaaccag agccgataaa agttaccgct
      541 gacgaagtaa acaaaattat gcaggcagcc aatatcagcc agcctgacgc cgataagttg
      601 cttgctgcat cacgtggtga atttgttgaa gggattagtg acccgaatga tccgaaatgg
      661 gttaagggga tccagacccg cgattctgtg aaccagaacc agcatgaatc ggaacggaac
      721 taccaaaaag cggaacaaaa cagtccaaat gcgttacaaa acgagccaga aacgaaacag
      781 cctgaaccag tggcgcaaca ggaagtggaa aaagcctgca ccgcctgcgg tcagaccggc
      841 ggcggcaact gccctgattg tggcgcggtg atgggcgacg caacatacca agaaacattc
      901 gatgaagagt atcaggttga agttcaggaa gatgatccgg aggaaatgga aggcgctgaa
      961 catccacaca aggagaacac tggcggcaat cagcatcaca atagcgataa tgaaactggc
     1021 gagacggcag atcacccaat taaggtgaac ggtcatca
//
LOCUS       DAZGLJ010000515         1056 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000515 DAZGLJ010000000
VERSION     DAZGLJ010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.528"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..309
                     /locus_tag="ACHK49_005279"
     CDS             <1..309
                     /locus_tag="ACHK49_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9265716.1"
                     /translation="WSYVSCGVYIYETDTAINVGIRNYGSGNVLINSPSLCAYGMPSN
                     ALQVEKTTFYLSSVPTSGFWDIGERVINSAPASGQPKAWTCNIPGGPGTYSFLSEGNF
                     "
     gene            380..>1056
                     /locus_tag="ACHK49_005280"
     CDS             380..>1056
                     /locus_tag="ACHK49_005280"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO9265717.1"
                     /translation="MERKTNKLDSIQVLRGVAVLLVIAFHFRVYLNGVYAQKDLGDIL
                     FGIGEVGVDIFFVISGFIITYSSMNKAKNRPMVFAAKRFFRLYPVYFIILTLAIYLNY
                     EEIYTSSQIIKSYLLIPIDYNFIGPWSGYSIIIPAWTLTYEIWFYVVFFFSLCISHKY
                     RTAIASFLLIIICVLGQLYFHGDVFLNPIAQPPSGDGIIRNLVFVSNPIIYDFILGMI
                     IAEAYIRL"
BASE COUNT          314 a          175 c          203 g          364 t
ORIGIN      
        1 tggagctatg ttagttgtgg tgtgtatata tacgaaactg atacagctat aaatgttggt
       61 ataagaaatt atggttcagg aaatgtactc ataaactcac cttctctatg tgcatatgga
      121 atgccatcaa acgcgttgca agttgaaaaa acaacatttt atctttcgtc agtacctaca
      181 tccggtttct gggatatagg tgagcgtgtt atcaatagcg cacctgcttc aggacaacca
      241 aaggcatgga catgcaatat tccaggtggg ccaggaacct attctttctt atcagaggga
      301 aacttttaaa agacctatgt attgaaatga ttactttgat ctggtatgtt taaaaaaaat
      361 cagtaaaaca cattttgata tggaaagaaa aacaaataag ttagactcta ttcaagtcct
      421 tagaggggtc gcggtacttc tagtcattgc gtttcacttt agagtatatt taaatggagt
      481 gtatgcccaa aaggacttgg gcgacattct ttttggtata ggtgaggttg gtgttgatat
      541 attctttgtt ataagtggat ttatcattac ttattcttca atgaataagg caaaaaaccg
      601 gccaatggtg tttgctgcta agcgtttttt taggttgtac cctgtttact ttataatcct
      661 tactctggct atatacctca actatgagga aatatataca tcatcacaaa taataaagag
      721 ctacttgctc ataccaattg actataattt tatagggcca tggtcaggtt atagtataat
      781 aatacctgca tggacactaa cgtatgaaat ttggttctat gtggttttct ttttttcatt
      841 atgcatcagc cataaatata gaactgcgat cgcatcattt cttctcatca ttatttgcgt
      901 tcttggtcaa ctgtactttc acggagatgt ttttctaaat cctatcgcgc aaccgccatc
      961 tggtgatgga attattcgta atctggtatt tgtatcaaat ccaataatat atgactttat
     1021 tttggggatg attattgcag aggcctacat taggct
//
LOCUS       DAZGLJ010000516         1055 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000516 DAZGLJ010000000
VERSION     DAZGLJ010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.529"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..1026
                     /locus_tag="ACHK49_005281"
     CDS             <1..1026
                     /locus_tag="ACHK49_005281"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO9265718.1"
                     /translation="RGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGNDLEIDGIGGG
                     NPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGAFAIENGLIA
                     ATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVALTFLNAAGT
                     KTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELDADKALLARI
                     ESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRALAITGAIAI
                     SSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRASVIRTTRKIF
                     SGEVYLP"
BASE COUNT          265 a          242 c          269 g          279 t
ORIGIN      
        1 cgaggtggaa cctcgagggg cgcgttcctg ttagcggaac atttacccga agatcaaacg
       61 cagcgcgata aaatattgat ggcaattatg ggttccggta acgatctgga aattgacggt
      121 attggcggcg gtaatccact gaccagtaaa gtcgccatta ttagccgttc cagcgatccg
      181 cgtgctgatg ttgattatct gtttgctcaa gttatcgtcc atgagcaacg tgtcgatacc
      241 acgcctaact gcggcaatat gctttctggc gttggggcat tcgccattga aaatggtttg
      301 attgcagcga catcaccggt cacccgtgtg cgtatccgca acgtcaatac gggtacgttc
      361 atcgaagctg atgtgcaaac gccaaatggt gttgtcgagt acgagggtag tgccagaatt
      421 gacggcgtac cgggtactgc cgcaccggtt gcgctcactt tcctgaatgc cgctggaacc
      481 aaaaccggaa aagttttccc gactgataat cagattgatt attttgacga tgtcccggtg
      541 acctgtatcg atatggcgat gccagtcgtc attattccgg ctgaatatct gggaaaaaca
      601 ggttatgaat taccggcgga actggatgcc gacaaagcat tattagcccg cattgaatct
      661 atccgtctac aagcgggtaa agcaatgggc ttaggtgatg tcagtaatat ggttatccct
      721 aaacctgtgc ttatttctcc agcgcagaaa ggcggggcaa ttaatgtgcg ttattttatg
      781 ccgcattctt gccatcgcgc gctggcgata accggtgcta ttgctatttc cagtagttgt
      841 gcattggaag gcaccgtcac ccgacaaatc gtcccttctg taggatacgg caatatcaat
      901 attgaacacc ccagtggtgc gctcgacgtt catttaagta atgaaggtca ggatgccacg
      961 acgttacgcg catctgttat tcggacgacc agaaaaatat tttccggtga agtttatctt
     1021 ccctgaaaaa attcgttgtc aagataagga taatc
//
LOCUS       DAZGLJ010000517         1048 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000517 DAZGLJ010000000
VERSION     DAZGLJ010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.530"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            23..970
                     /locus_tag="ACHK49_005282"
     CDS             23..970
                     /locus_tag="ACHK49_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO9265719.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
BASE COUNT          298 a          200 c          248 g          302 t
ORIGIN      
        1 ttcattgaag tgagtgtcag ctatgaaaaa tgttctggtc accgtcccca gcttttcagc
       61 acgctgtgtt tccgccagca agttgctaag agagaataat tttaatctta ttattaagaa
      121 taatgtggag catctgctta aatcagaatc cactgcatta cgagaatcta tttgcgcggt
      181 catcgccgga aaagattgtt atcaggcgga cacgctatca ttgctgcctg gtgtgaaaat
      241 aatatcgaga ttcgggacgg ggatagataa tattgatctt cgtgcagcac aacaatccgg
      301 tattgtagtc aataatgcgg ttgggattaa ttcaaatgct gtcgcggaat ttatcattgg
      361 acttatcttc gcgagcatga gaaatattcc aggcagctat catgcgatgc aaaatggcta
      421 ctggggtgag tcgcatggct atgaattaca aggcaaacgg attggcatgg ttggctacgg
      481 caatataggt aaaaccctgg caaaaaggtt atccggtttt gatgttgagc ttttagctta
      541 tgacaaacaa cccgattatc aggttgctga caaagttgga gttcagtttg tatcaattga
      601 agatatcttt atgcagtcgc atgtcatcat tgttcttttg cctttttctc ctgagctgga
      661 gaacttcatt agtcataaat atttgtcgat gatgcgcaat ggtgctctaa tcatcaatgc
      721 cgcaagggga aagttgctgg atgagggcgc tttactacag gtgattgaag aacgaaatgt
      781 gtttgcggcg ctggatgtgt ttagctctga accgttggcg caatttagcc cactgctaca
      841 tgccaaaaat atcattacaa cgccgcatat tgcggccgcc accgttgagt cataccagca
      901 aaccggtaca cacgttgccc agtcgataat tgattacttc gcaggaaggg agataaaaaa
      961 cgtgttgtga gttcagcgaa cgaagttttt aaatacaaaa aaaaccgcaa tttgtggaac
     1021 acattgcggt ttatcagcat tctaagta
//
LOCUS       DAZGLJ010000518         1044 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000518 DAZGLJ010000000
VERSION     DAZGLJ010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.531"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            59..205
                     /locus_tag="ACHK49_005283"
     CDS             59..205
                     /locus_tag="ACHK49_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265720.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFLVMLVAIFFSTAWRVLTLS
                     GLVG"
     gene            433..639
                     /locus_tag="ACHK49_005284"
     CDS             433..639
                     /locus_tag="ACHK49_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO9265721.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            704..928
                     /locus_tag="ACHK49_005285"
     CDS             704..928
                     /locus_tag="ACHK49_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265722.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
BASE COUNT          255 a          238 c          289 g          262 t
ORIGIN      
        1 tgctgattga ttttgtcctg gctgatacag gcaaagggaa aataacaaag gcggattgat
       61 ggggtggcta atgatatcag ataaactcat aacgctggtg aagagcctct gtgtacttgt
      121 cggcatttca tttttagtca tgctggttgc cattttcttt tccaccgcct ggcgagtcct
      181 gacgttatcg ggactggtgg ggtgaaagag agatgaaccg tgttctgtgt gtggtgatta
      241 ttgtcatggc ggttggctgt ggtgcgctgt ggctggcaac aaaccattac cgtgacaacg
      301 cgctcaccta caaagcgcag cgcgataaaa aagccagaga gctggaacag gcgaatgcca
      361 ccattactga catgcaggtg cgccagcgtg atgttgctgc gctcgatgca aaatactcga
      421 gggaattagc cgatgcgaga gctgaaaatg aaactctgcg tgctgatgtt gccgctggtc
      481 gtaagcgcct gcggatcaac gccacctgcc ccggtaccgt gcgtgaagcc accggcacct
      541 ccggcgtggg caatgatgcc gccgtcgaac tctctccggt tgctggacga aacgttctcg
      601 gtatcagaga cggaatcatc agcgaccagg cagcattgag aatgcttcag gaatatatcc
      661 gcactcagtg tattaactag tatttttgtt attcggagaa tgcatgaaga aattattggt
      721 aaccgtaaag ccttttcagg gaacaattcc gttccgtatt ttgcagcgtg gtcgtgttct
      781 tgttgaaggt tcgttcagtg gtaaatgtac gcaattacac tccaggacct ttcaggtgaa
      841 tgccacgaat gaagagctaa ccgttgagtg tacgatgaat gccgctaaat gccgcatggt
      901 atccgctgca ttacagccag tgtgttgagc gaccttatta ttcatgcgcg gtattgtcgc
      961 cgtattcctg cattaacaga gaccgcagcc cgaccgggag aatcctctgc gcgagtgtgc
     1021 ggggataatc aaaaacgata caca
//
LOCUS       DAZGLJ010000519         1041 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000519 DAZGLJ010000000
VERSION     DAZGLJ010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.532"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..513
                     /locus_tag="ACHK49_005286"
     CDS             <1..513
                     /locus_tag="ACHK49_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO9265723.1"
                     /translation="SNTLTGYVVRWDKLSELLWGEFYEKFQRGAFTEWLAAGNDVRGL
                     YEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTGRDVIELVKRGDISGMSFGFR
                     SRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGVELARRSLYEQHPEKMPRADN
                     RRWWADLAGV"
     gene            515..>1041
                     /locus_tag="ACHK49_005287"
     CDS             515..>1041
                     /locus_tag="ACHK49_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9265724.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLNGNAYAVIQWGRDGFPAALHPYPPQSVNMEQTGEHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDG"
BASE COUNT          231 a          270 c          314 g          226 t
ORIGIN      
        1 agcaacacgc ttacaggcta cgttgttcgc tgggataagc tttcagaact gttatggggt
       61 gagttttacg aaaaattcca gcggggggcg tttactgaat ggcttgcggc gggtaatgac
      121 gttcgcggcc tgtatgagca tgaccacagt atgttactgg ggcgcacccg ttccggcacg
      181 ctgaaactgg aagaggatga aacagggtta cgctttgaac tgaccccacc ggataccagt
      241 acagggcgcg acgttatcga actggttaag cgtggtgata tatccggcat gagctttggc
      301 tttcgttccc gtaaggatgt atgggatacc acaacagatc catgcgtgcg caccgtgctg
      361 gttgcggaac tgtacgaaat taccgtcaca tccgtaccgg cttaccctga ttccggcgtg
      421 gagctggccc gccgctccct gtacgagcag caccccgaaa aaatgccgcg tgcggataat
      481 cgccgctggt gggcggattt agcgggggtg tgatatgtgg cctttcagaa gaaaaaaaga
      541 gcagcgcagc atgacgcttg atgagtttat ggcgctggct ggcacatcga acacgggggc
      601 gggtgagtac gtatcatcgg ggacagcgga atcactgccc gccgtcatga acgccgtgac
      661 ggtcatctct gaggcggtgg ctaccatgcc gtgttacctg tacctggtac gcaatgagaa
      721 ggggaaggag gcccgcgagt ggcttgattc tcatccggtc gatcacatcc tcaacgagcg
      781 cccgaacgcg tggcagactc cctaccagtt taagcgaatg atgatccgcc actgcctgtt
      841 aaacggtaat gcttacgcgg tgattcagtg ggggcgtgat ggttttccgg cggctttgca
      901 tccttacccg ccgcagtcgg tgaacatgga gcagacagga gaacacaact ggcgctactg
      961 catcactgac gcttacaccg gaaacacccg caactattta ccgtgggagg ttctccacct
     1021 tcgttactcc acggatgacg g
//
LOCUS       DAZGLJ010000520         1041 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000520 DAZGLJ010000000
VERSION     DAZGLJ010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.533"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            10..513
                     /locus_tag="ACHK49_005288"
     CDS             10..513
                     /locus_tag="ACHK49_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001454006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO9265725.1"
                     /translation="MTGYVVRWDKLSELLWGEFYEKFQRGAFTEWLAAGNDVRGLYEH
                     DHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTGRDVIELVKRGDISGMSFGFRSRK
                     DVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGVELARRSLYEQHPEKMPRADNRRW
                     WVDLAGV"
     gene            515..>1041
                     /locus_tag="ACHK49_005289"
     CDS             515..>1041
                     /locus_tag="ACHK49_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9265726.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLNGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGEHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDG"
BASE COUNT          234 a          270 c          310 g          227 t
ORIGIN      
        1 gccggtacgc tgacgggcta cgttgttcgc tgggataagc tttcagaact gttatggggt
       61 gagttttacg aaaaattcca gcggggagcg tttactgaat ggcttgcagc gggtaatgac
      121 gttcgcggcc tgtatgagca tgaccacagc atgttactgg ggcgtacccg ttccggcacg
      181 ctgaaactgg aagaggacga aacagggtta cgctttgaac tgaccccacc ggataccagc
      241 acagggcgcg acgttatcga actggttaaa cgtggtgata tatccggcat gagctttggc
      301 tttcgttccc gtaaggatgt atgggatacc acaacagatc catgcgtgcg caccgtgctg
      361 gtggcggaac tgtacgaaat taccgttaca tccgttccgg cttaccctga ttccggcgtg
      421 gagctggccc gccgttccct gtatgagcag caccccgaaa aaatgccgcg tgcggataat
      481 cgccgctggt gggtggattt agcgggggtg tgatatgtgg cctttcagaa gaaaaaaaga
      541 gcagcgcagc atgacgcttg atgaatttat ggcgctggct ggcacatcga acacgggggc
      601 gggtgagtac gtatcatcgg ggacagcgga atcactaccc gccgtcatga acgccgtcac
      661 ggtcatctct gaggcggtgg ctaccatgcc gtgttacctg tacctggtac gcaatgagaa
      721 ggggaaggag gcccgcgagt ggcttgattc tcatccggtc gatcacatcc tcaacgagcg
      781 cccgaacgca tggcaaaccc cctaccagtt taagcgaatg atgatccgcc actgcctgtt
      841 aaacggtaat gcttatgcgg tgattcagtg ggggcgtgat ggttttccgg cggctttaca
      901 tccttacccg ccgcagtcgg tgaacgtgga gcagacaggc gaacacaact ggcgctactg
      961 catcactgac gcctacaccg gaaacacccg caactattta ccgtgggaag tacttcacct
     1021 tcgttactcc acggatgacg g
//
LOCUS       DAZGLJ010000521         1036 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000521 DAZGLJ010000000
VERSION     DAZGLJ010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.534"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..942)
                     /locus_tag="ACHK49_005290"
     CDS             complement(<1..942)
                     /locus_tag="ACHK49_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO9265727.1"
                     /translation="MKAIDNIFKYVPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNDRSHQMEKVWFYNSEPSIDIQEYNRFADNT
                     TDTFIFTIIPDNNHVLKLSSPITVTVECKGGYYFINSSGDKSDITYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFAGYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYALLVSGHIGIREKGWSLLCELLNHENMASSAYKHKNNKVLYELLNTRD
                     MILNELHKHVFLKDDAITPCIFLGDHTGDRFSTIFGDK"
BASE COUNT          341 a          190 c          158 g          347 t
ORIGIN      
        1 tttatcacca aaaatagtac taaatctatc gcctgtatga tccccaagga agatacaagg
       61 ggttatcgca tcgtccttta aaaaaacatg tttgtgcaac tcattcaata tcatgtctct
      121 cgtatttaat aactcgtaga gcactttatt atttttatgc ttgtatgcag atgaagccat
      181 gttctcgtga tttaataatt cacataataa actccaaccc ttttccctga tgcctatatg
      241 ccctgaaacg agcaatgcat aaagtacagc gcctactctt ccatctgtat cgccaaaata
      301 agccgtgttg gcattttgtg ggttaatcac accacattgt ttataaaagt ctattttaga
      361 aaacacagat ttaacatagc cagcaaactt atccttagga aacgtctctt tagagacatc
      421 ccaacgccag tcagctttaa aattaccgct aagaagagta aagaaatttt caggtatgat
      481 agaaagccca ttaactttat aagttatgtc tgatttatct ccagaggaat taataaaata
      541 ataaccgccc ttacactcaa cagtaacggt aatgggagac gataatttta acacatggtt
      601 attgtctggt ataattgtaa agataaacgt gtcagtagta ttgtcggcga atctattata
      661 ttcttgaata tctatagaag gttcactatt gtaaaaccat actttctcca tctgatgact
      721 acgatcattt accctccagt gaccatgttg caggaaatag atgatatgca tgccaaaatc
      781 gttatctatt tgcgaatcat tatccagttt atcaatttca actatctttg aacgatgtgt
      841 tttatcaacc ttgctatggt gttgatgatt attaacttta tctgaatgct tatctttatg
      901 gtttgaaggg acgtacttaa atatattgtc aattgctttc ataaataacc tgcgatattt
      961 ttgacttgtt agtaacaaaa taaataatac atgtctgaaa gacatatttt cgacgatata
     1021 aaacaataat acttat
//
LOCUS       DAZGLJ010000522         1033 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000522 DAZGLJ010000000
VERSION     DAZGLJ010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.535"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>1033
                     /locus_tag="ACHK49_005291"
     CDS             <1..>1033
                     /locus_tag="ACHK49_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISCro1 family transposase"
                     /protein_id="HFO9265728.1"
                     /translation="EYNRLLSQRVAAYASEINRLKALVAKLQRMQFGKSSEKLRAKTE
                     RQIQEAQERISALQEEMAETLGEQYDPVLPSALRQSSARKPLPASLPRETRVIRPEEE
                     CCPACGGELSSLGCDVSEQLELISSAFKVIETQRPKQACCRCDHIVQAPVPSKPIARS
                     YAGAGLLAHVVTGKYADHLPLYRQSEIYRRQGVELSRATLGRWTGAVAELLEPLYDVL
                     RQYVLMPGKVHADDIPVPVQEPGSGKTRTARLWVYVRDDRNAGSQMPPAVWFAYSPDR
                     KGIHPQNHLAGYSGVLQADAYGGYRALYESGRITEAACMAHARRKIHDVHARAPTYIT
                     TEALQRIGEL"
BASE COUNT          240 a          292 c          299 g          202 t
ORIGIN      
        1 gaatacaacc gcctgctctc acagcgggtg gcggcttatg cttccgaaat caaccggctg
       61 aaggcgctgg ttgcgaaact gcaacgtatg cagttcggta aaagctcaga aaaacttcgt
      121 gcaaaaaccg aacggcagat acaggaagca caggagcgaa tcagcgcact tcaggaagaa
      181 atggcggaaa cgctgggtga gcaatatgac ccggtactgc catccgccct gcgccagtct
      241 tcagcccgta aaccgttacc ggcctcactt ccccgtgaaa cccgggttat ccggccggaa
      301 gaggaatgct gtcctgcctg tggtggtgaa ctcagttctc tgggatgtga tgtgtcagag
      361 caactggagc ttatcagcag cgcctttaag gttatcgaaa cacaacgtcc gaaacaggcc
      421 tgttgccggt gcgaccatat cgtgcaggca ccagtacctt caaaacccat tgcacgcagt
      481 tatgccggag cggggcttct ggcccatgtt gtcaccggga aatatgcaga ccatctgccg
      541 ttataccgcc agtcagaaat ataccgtcgt cagggagtgg agctgagccg tgccacactg
      601 gggcgctgga caggtgctgt tgctgaactg ctggagccgc tgtatgacgt cctgcgccag
      661 tatgtgctga tgcccggtaa agtccatgct gatgatatcc ccgtcccggt ccaggagccg
      721 ggcagcggta aaacccggac agcccggctg tgggtctacg tccgtgatga ccgtaacgcc
      781 ggttcacaga tgcccccggc ggtctggttc gcgtacagtc cggaccggaa aggtatccat
      841 ccacaaaatc acctggccgg ttacagcggt gtgcttcagg ccgatgctta cggtggttac
      901 cgggcgttat acgaatccgg cagaataacg gaagccgcgt gtatggctca tgcccggaga
      961 aaaatccacg atgtgcatgc aagagcgccc acctacatca ccacggaagc cctgcagcgt
     1021 atcggtgaac tgt
//
LOCUS       DAZGLJ010000523         1026 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000523 DAZGLJ010000000
VERSION     DAZGLJ010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.536"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..332)
                     /locus_tag="ACHK49_005292"
     CDS             complement(<1..332)
                     /locus_tag="ACHK49_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO9265729.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGISAQFTSGAQRGGEVQGVFD
                     DPESLGFASSGIRIEGSSPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPP"
     gene            complement(325..699)
                     /gene="gpFI"
                     /locus_tag="ACHK49_005293"
     CDS             complement(325..699)
                     /gene="gpFI"
                     /locus_tag="ACHK49_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO9265730.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEMQS
                     SGDDVQVMNMDIRERENAAHDVREETSGALTRIRVLTCLHLCGVDGETGESVELADVG
                     RVILIMSSDAKTHVDGGMAVYA"
     gene            complement(751..>1026)
                     /locus_tag="ACHK49_005294"
     CDS             complement(751..>1026)
                     /locus_tag="ACHK49_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO9265731.1"
                     /translation="VWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGAVVSFKGYYGD
                     LAIVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK"
BASE COUNT          249 a          340 c          222 g          215 t
ORIGIN      
        1 ggtggttgcc cacggttcag ccagagataa caactgcccc cgtcatccgg agaaacacga
       61 tccacccaga acgtctcacc attaatggtc aacgtgtcac cacgccgcac ggcacgaacc
      121 gtatccgtcc gcacaaataa tgacgggctg cttccttcaa tacggatccc actactggca
      181 aaccccagcg actccggatc gtcaaaaacc ccctgaactt cgccgccacg ctgtgcaccg
      241 gaggtgaact gcgcactgat gcccatcact tcaacaatcg tactgtccac cccggcaagg
      301 gcggcatcaa aggcattctg aaaatcacgc ataaacagcc attccaccat caacgtgtgt
      361 ttttgcatct gaggacataa tcagaatcac ccgaccaaca tccgcaagct caacggattc
      421 ccccgtttca ccatcaacgc cacagagatg gaggcaggtc agaactctga tgcgcgttaa
      481 cgcgccggat gtttcctcac gaacatcatg agccgcgttt tccctctccc ggatatccat
      541 attcataacc tgtacatcat cgccggatga ctgcatttcc tcttcccatt ctgccacccg
      601 ctgcgctatc tctgcggcac tcccggatat atccggctca cgccccagaa tcagggccag
      661 ttcatcaagc cgtttcagat tttgctcttt cgttgccata tcatccccct gtgaaaaaag
      721 acacgggggc atttcgcccc cgctcacgga ttatttcacc tgtaccacca caaactcatc
      781 cgggtccggc aacaccatca gcggcgcgga ctgcgtcatg gtaaattcac gggcgggatc
      841 ccctaccgtc agccagtgtt tcggataacg ggaagaggcc accacaatgg ccagatcgcc
      901 gtaataccct ttgaaggaca ccaccgcgcc caggtctttc actgccgttt cgagttgtga
      961 atttgagccg cgacgggtat ccagtttttc gcggaacaat ttaaagccat tcagcagacg
     1021 ccagac
//
LOCUS       DAZGLJ010000524         1022 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000524 DAZGLJ010000000
VERSION     DAZGLJ010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.537"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(113..802)
                     /locus_tag="ACHK49_005295"
     CDS             complement(113..802)
                     /locus_tag="ACHK49_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO9265732.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(817..939)
                     /locus_tag="ACHK49_005296"
     CDS             complement(817..939)
                     /locus_tag="ACHK49_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO9265733.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
BASE COUNT          268 a          261 c          194 g          299 t
ORIGIN      
        1 gcagccagca tgtttaccgt acaagtacaa ctcagggcat aaaaaaaacc cactcggcag
       61 cgggtttata cattttttac aacataccaa atttgcatga agtatatggc ttttaatcca
      121 gttttgcaat attttgctgt aaaaatgctg ccttttgttt tgaacgtgtt ctcgtcacaa
      181 gcaataaagc atcactatca agctgtagaa aaatgtgctt cattgcaacc cagcgttcag
      241 taaatgtctc ggaccagttt tttgttgtca ctcccaccaa tgatgccagt gtctggtatt
      301 cataggcctc acgccctgca agttcgctct tcacatcctg tgcagccagc cagattaacg
      361 tcttcaggcg atccagtgtc ttacctgcaa tttttctgtt acttaacaaa tctttaaact
      421 cgctccatgc ccattgcgtt atggtgacct gatgccccca tcgaacgctt tcgctgtaac
      481 accaaagcaa ccatgctttc tgatgttcat cgagagacaa aaccgcgcgg cgccatgaag
      541 aggttgagaa ttcaaccggg ctgaccaaag caatggatga accttttgcg tacgactgct
      601 taccggaagt cggcgtatta tccagcgtaa tcatcttgcc agttaccaca tccagaatgc
      661 gcggcttctt tcgtttgtat gtaccagtat caaattgtgc atgctcctgc caggcttcga
      721 gctggccttt cgttgctccg ttcaagtcag cagtagctgc cataagttgc tcacgaacat
      781 actgtaaata ttgggtattc atgcagtaaa tcctttctat attttggcat aattcttcaa
      841 cattcggtaa tcgttcaaaa ccgaatcggg gaaacgacat aagcacagga gcccccagcg
      901 acagcgaagg agttctgata tataagactc agacatcatt cattccccgg ttctccaata
      961 tctgtttcac tcatcatcca taactacctg taattgcccc cccttttttt gtaacagttc
     1021 tt
//
LOCUS       DAZGLJ010000525         1022 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000525 DAZGLJ010000000
VERSION     DAZGLJ010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.538"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..353)
                     /gene="yjdN"
                     /locus_tag="ACHK49_005297"
     CDS             complement(<1..353)
                     /gene="yjdN"
                     /locus_tag="ACHK49_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO9265734.1"
                     /translation="MSLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQ"
     gene            complement(513..848)
                     /locus_tag="ACHK49_005298"
     CDS             complement(513..848)
                     /locus_tag="ACHK49_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO9265735.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT          247 a          239 c          258 g          278 t
ORIGIN      
        1 tcctgccagg ccatttcgat ttttccgtta gcggcaagat tgtcaaacca gcgttttcct
       61 tcttcgacct gttgcgaatc gagcaccagc gtaaagccgg agtagctggc ttttcctgac
      121 ggtatagcat cgctcatcat gatgtcgctt ccggcaatgc gcacgttggc gtgggcgatg
      181 gcggtatcgg gaaattgcat tccggaaggg cagttctcgg cgctgtcctg cgctgatttt
      241 ggcatttcgc cgaagctgat tttatagagc agttcagcgc ccaacgtacg ttgataataa
      301 gcaattgcgt cggcacagtt accggcaaaa gagaggtagg gacttaacga catgatggtg
      361 acctcagtta agagaagcca gttaagtgta gttcgaaatt tatacagatg agagggaagc
      421 ctgataagac gcggtcagcg tcgcatcagg cagtcggcat cgttgccgga tgcggcgcga
      481 gcgccttatc cggcttaccg atcacaatac aatcagttct ttttcacaaa ctcagatttc
      541 agtttcatcg gaccaaaacc gtcgattttg caatcgatgt tatggtcgcc ttcaaccagg
      601 cggatgtttt tcactttggt gccaattttc agcatcgaag agctaccttt caccttcaga
      661 tctttaatga tggtaacgct gtcgccgtca gccagcagat tgccgttagc atctttaacg
      721 atcagctcgt cgctttcctg tgcaggttct gcgtcgttcc attcgtaggc acattccggg
      781 cagatgtaca tgccgttatc ttcgtaagtg tattcggagt tgcattttgg gcagtgtggt
      841 aatgacatgt ggatttcctc aaaagtcagc agggcaaaag cgccaaaaaa cggcagattg
      901 ccgaaaaagg ccgcaattat acacaaaatc cctgagtttg tcgggggtac tgctgatttt
      961 agacgacgat cgttttcatt tgaaaggaaa cgcaaacaga aaataatctt tttgcctcca
     1021 tt
//
LOCUS       DAZGLJ010000526         1008 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000526 DAZGLJ010000000
VERSION     DAZGLJ010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.539"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..612
                     /locus_tag="ACHK49_005299"
     CDS             <1..612
                     /locus_tag="ACHK49_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 34 domain-containing
                     protein"
                     /protein_id="HFO9265736.1"
                     /translation="NQLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLRGRYI
                     TQDPIGLKGGWNFYQYPLNPVTNTDPLGLEVFPRPFPLPIPWPKSPAQQQADDNAAKA
                     LTKWWNDTASQRIFDSLILNNPGLALDITMIASRGNVADTGITDRVNDIINDRFWSDG
                     KKTDRCDVLQELIDCGDISAKDAKSTQKAWNCRHSRQSNDKKR"
     gene            633..1008
                     /locus_tag="ACHK49_005300"
                     /pseudo
     CDS             633..1008
                     /locus_tag="ACHK49_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315903.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
BASE COUNT          339 a          186 c          231 g          252 t
ORIGIN      
        1 aaccagctga atgaagagaa cccgcaccag ctgcagcagc tcatccgcct gccggggcag
       61 cagtatgatg aggagtccgg cctgtattat aaccgccacc gctattatga cccgctgcgg
      121 gggaggtata tcactcagga tccgattggg ctgaaggggg gatggaattt ttatcagtat
      181 ccgttgaatc cagttacgaa tacagatcct ctggggttag aagtttttcc tagaccattc
      241 cccttgccaa ttccatggcc caaaagccct gcacagcagc aagcagatga taatgctgca
      301 aaagcattga caaaatggtg gaacgataca gcatcacaaa gaatatttga ctctctaata
      361 ttgaataatc cgggactagc attagatata acaatgatag cttctcgtgg aaatgttgca
      421 gacacaggga taactgatcg tgtcaatgac ataataaatg acagattctg gagtgatggg
      481 aaaaaaaccg acagatgtga cgtacttcag gaactaattg attgtggtga tattagtgct
      541 aaagatgcaa aaagcacaca gaaagcctgg aattgtcgtc actccagaca gtcaaacgat
      601 aaaaaaagat agcccttgtg gaggttcctg caatgtcaaa tacataccag aaaagaaagg
      661 caagtaaaga gtatggttta tataataaat gtaagaaact aaatgatgat gaattatttc
      721 gcttacttga tgatcgcaat tccttgaaaa ggatttcatc tgccagagta ttacagttaa
      781 gaggggggca agacgctgtt agattggcaa ttgaattctg cactgacaaa aattatatcc
      841 gtagagatat cggagcattt atactcgggc aaatataaat ttgcaaaaaa tgcgaagata
      901 atgtttttaa tattttgaac aatatggcat tgaatgataa gagtgcttgc gttcgagcta
      961 cggcaatcga gtcaacggcc agggaaagat catcaaaagt tcactttt
//
LOCUS       DAZGLJ010000527          992 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000527 DAZGLJ010000000
VERSION     DAZGLJ010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.540"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(111..>992)
                     /locus_tag="ACHK49_005301"
     CDS             complement(111..>992)
                     /locus_tag="ACHK49_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9265737.1"
                     /translation="FPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVM
                     RYAVQSGIIRYNPALDMAGALTTVKRQHRPALNLSRLPELLSRIDGYKGQPVTRLAVM
                     LNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSK
                     QAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAM
                     ACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRE
                     KGISPFEYANVNNPLRR"
BASE COUNT          243 a          240 c          249 g          260 t
ORIGIN      
        1 tatttagagg taacgggtga ttgcggtgct atcagccgga ataaaaaaga aatttattgg
       61 agcaggttgc tcttccccat gggaagagca tgtgtgtgaa tagccttctg tcaccttctc
      121 agcggattgt taacatttgc gtattcaaac ggactgatcc ccttctcccg gttcgcatca
      181 agaaaatcgg cccaccactg tagcatcagt cgacgttctt ccagatgttc tgctttatgg
      241 atatacgcag cacgtacacc attacgctcc tgatggctca tctgacgttc cacagcatcg
      301 cgggaccaca aacctgattc aatcaatgca ctgcacgcca tcgcccggaa tccatggcca
      361 cagacatcct gggttgtgtc ataccccatc accctcaggg ctttatttac agtattttca
      421 ctgattggtt tacgcgggtc atgtgctccc gtaaagatca gaccattttc accagcccat
      481 gtctgtagtt ctgtcagtat ggctacagcc tgtttgctga gtggcacaag atgtggtgtt
      541 cgcatcttgg agccccggtg tgagaatttt acgcctggca gaggttcgcg ttcggctgga
      601 atagtccaca tggcattgtc aatatcaatt tcagaccagc gggcatatct gagttcactg
      661 gaacgaataa aaaccagtaa attcagcata acggcaagcc gggtgacagg ctggccttta
      721 taaccgtcaa tacgcgacag aagttcaggc aggcgtgaaa gattcagagc ggggcgatgc
      781 tggcgtttta ccgtggtcaa tgcaccagcc atatccagag ccgggttata tctgatgatg
      841 ccagactgta cggcgtaacg cattacagca gatattcttt gctgcagacg actggcgatt
      901 tcataaattt gttttgcttc tgcagcacga acagggataa gcaggtccgg ggtattgagt
      961 gttgtgatat cccggttgcc gataaagggg aa
//
LOCUS       DAZGLJ010000528          989 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000528 DAZGLJ010000000
VERSION     DAZGLJ010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.541"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..95
                     /locus_tag="ACHK49_005302"
     CDS             <1..95
                     /locus_tag="ACHK49_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265738.1"
                     /translation="AFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            109..390
                     /locus_tag="ACHK49_005303"
     CDS             109..390
                     /locus_tag="ACHK49_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265739.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRALELLAYRRRVEMYREKEKITYHWKKPERLRR"
BASE COUNT          279 a          220 c          262 g          228 t
ORIGIN      
        1 tggcgtttgg tacattccag gcagagcatg aggattttgc ttttgatata agcgacagcc
       61 accgccagaa actggaagaa tttagcgggg tgtaatgagg tcagcagcat gacagaagcc
      121 gaaataacgg gattaatccg ccgcgttgcc ggaatcagcc agcaggctga cgaacaggcc
      181 acgcagccgg acagcgtgac agccgaaaac tatgcgcgtg ttgttgctga ggtgatgcgc
      241 cgtgatggta tagaacttaa tggcgtggat atgcgcaaca tacgaaccag agctcttgag
      301 ttgctggcct accgtcgccg cgtggagatg tatcgggaga aggagaaaat aacgtaccac
      361 tggaagaagc cggagcggtt gcggcggtaa ctggttgata ttcccgacgg accaaaaatg
      421 ggtggctaca tccctcgctt ctgaggactg ttaacaagct gcctgaaaga cagtcacaaa
      481 ctacgaaatt ttcgcagttt aattaatgat cagcttaatc gctaacccgc tgatattttc
      541 ggaaacctca atttgaggaa gtcggcgcgg taactcgctg aactttaagc aaagtgcaaa
      601 attgcgcttg ctgaataatc attatgatta cgcagatgat taaggaatga ccgaaggcgg
      661 aaattcgcct gtggttaatg ggggagttgc agatctgcaa ctcgaccatg aaactacgga
      721 aactacccgt agtttgggta gtaagagtaa cacccagatt ttgggtctta ctcgtgatac
      781 ccaaatttgg ggtatcagtg gcaaccataa cgactttcgt tacggttgat gcttttaccc
      841 cattggggga aaggtattac gataatcata acacctaccg aacaggtaaa gcccaccagc
      901 ctgattaacg tttaaccgga aaaaaattcc ggtaggtggg gatccccata tcgacattaa
      961 cgcccctcat gaattggtgc gcttccccc
//
LOCUS       DAZGLJ010000529          983 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000529 DAZGLJ010000000
VERSION     DAZGLJ010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.542"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..558
                     /gene="tnpA"
                     /locus_tag="ACHK49_005304"
     CDS             <1..558
                     /gene="tnpA"
                     /locus_tag="ACHK49_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO9265740.1"
                     /translation="PKSTVCSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVV
                     RPESTVISEAPVVKKRPRRPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNW
                     RHQYRKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAG
                     TLRLKGKLTPALLQTLIREIKGSSH"
     gene            558..905
                     /gene="tnpB"
                     /locus_tag="ACHK49_005305"
     CDS             558..905
                     /gene="tnpB"
                     /locus_tag="ACHK49_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9265741.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
BASE COUNT          235 a          279 c          259 g          210 t
ORIGIN      
        1 ccaaaatcaa cagtttgtag tatgttcgtg cgctttcgga gagctggcct ttcgtggcct
       61 ttgcccgcag gcatgtcgga gcaggaactt gatgcctgcc tttacggaca attttccacg
      121 gtaccagtcg tacgtcctga aagcaccgtt atatccgaag cccccgtggt aaaaaaacgt
      181 ccccggcggc ccaacttccc ttatgagttt aaaatcgcct tagtggagca gtcactgcag
      241 cccggagcct gtgtggcgca gatcgcccgg gaaaacggaa tcaacgataa cctgctcttc
      301 aactggcgcc atcaataccg gaaaggtggc ctgctgcctt ccggaaaaaa tatgccggca
      361 ctgcttcccg tgacgttaac gccggagccg gataataaaa tcccggcccc cgcacaggaa
      421 ccagagcaga taaatacacc gtccgacagt ctgtgttgtg agctggttct gccggccgga
      481 actctcaggc ttaaaggtaa actgacgccg gcgttattac agacacttat ccgcgaaata
      541 aaagggagca gccactgatg atatctctcc ctgcaggttc gcgtatctgg ctggttgcag
      601 gtatcaccga tatgcgaaat ggctttaacg gcctggcatc aaaagttcag aacgtcctga
      661 aggatgaccc gttctccgga cacctgttca tcttccgcgg acgccggggt gaccagataa
      721 aagtgttgtg ggctgacagt gacggactgt gcctcttcac caaacgcctg gagcggggcc
      781 gcttcgtctg gccagtcacc cgtgacggca aggtgcacct tactccggct cagttatcca
      841 tgcttcttga aggtatcaac tggaagcacc cgaaacgaac ggaacgcgct ggaatccgca
      901 tataacccgt tgtaaagtga ggatatggac acctcacttg ctcatgagaa cgcccgcctg
      961 cgggcactgt tgcagacgca aca
//
LOCUS       DAZGLJ010000530          982 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000530 DAZGLJ010000000
VERSION     DAZGLJ010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.543"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..404)
                     /locus_tag="ACHK49_005306"
     CDS             complement(<1..404)
                     /locus_tag="ACHK49_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265742.1"
                     /translation="MLKSFCLNFDGMYLSIVSFLSEKFNLKRVGKMELESKEDYEETT
                     HHNVASSVVTATHDNLDGREFFELCSNPALVFSFETHKKFNELPVDSKESPLDLFIAN
                     TKQLHLLRSGDISNTLSHLLLLGYVSAAESYLR"
     gene            complement(382..>982)
                     /locus_tag="ACHK49_005307"
     CDS             complement(382..>982)
                     /locus_tag="ACHK49_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO9265743.1"
                     /translation="IEKNDNVYFSRLTKLLIETGLFYNLPDISHGDGRTYERYIPHYA
                     LLIKERVFNEGSRGFSPSQIIQKILRKNAKHPVRRNISSLFNAEQLARLKLTLPPCNN
                     CKTPRLTDNQKFCHHCGNQLIDESAYNQCMSIPLSKVPHLTSWQMQKLSGLEKVKTIG
                     DVLSLQDPGSELRKIHRIGPKIANKIMDKVLLHVEEFLS"
BASE COUNT          313 a          179 c          159 g          331 t
ORIGIN      
        1 cgcaaataac tttcggctgc agacacataa cctaataaca acagatggct taatgtgttt
       61 gaaatatcac cagatcttaa caagtgaagt tgctttgtat tagctataaa aagatctaac
      121 ggggattcct tgctatcaac aggtaactca ttaaattttt tgtgtgtctc aaacgaaaaa
      181 actagagctg gattactaca tagttcaaaa aactctcttc catctaagtt atcatgggtt
      241 gcagtgacaa ctgaggacgc cacattatga tgagtggtct cttcataatc ctcttttgat
      301 tctagttcca ttttcccgac tctttttaaa ttaaactttt cagataagaa agacacgatt
      361 gacaagtaca tgccatcaaa attaagacaa aaactcttca acatgtaata acactttgtc
      421 cataatttta ttggcaattt tgggtcctat tctgtgaatt tttcttaact cactcccagg
      481 gtcttgtaaa gacaatacat ctcctatagt ttttactttt tccagcccag atagtttttg
      541 catttgccaa gatgttagat gtggtacttt tgacagtgga atagacatgc actgattata
      601 ggccgactca tcaatcaatt gattcccaca atgatggcaa aatttttgat tgtcagtcaa
      661 tcgcggagtc ttgcagttat tgcatggagg taaagttagt ttaagtctcg caagttgctc
      721 agcattgaat aatgatgaaa tgtttcttct gacaggatgc ttagcattct ttcttagtat
      781 tttttgaata atctgagaag gagaaaaacc tctacttcct tcgttaaaca ctctttcctt
      841 tattaataaa gcataatgag gtatatatct ttcataagta cgcccatcac catgagaaat
      901 atcaggaaga ttataaaaaa gccctgtttc aattaatagc ttagtgagtc gagaaaaata
      961 aacattatca tttttctcta ta
//
LOCUS       DAZGLJ010000531          976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000531 DAZGLJ010000000
VERSION     DAZGLJ010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.544"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(160..792)
                     /locus_tag="ACHK49_005308"
     CDS             complement(160..792)
                     /locus_tag="ACHK49_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO9265744.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          240 a          217 c          205 g          314 t
ORIGIN      
        1 acgggattat tgggatttac tttgaggaag ataaaatgcg tgtttataga accgcgagcg
       61 ccaaataatt ttatgaccac aacctgtcgt attgtggtca taaaaaattg tcggctggag
      121 ggagttaaat cctttacctc aatatactgc ctgtcagcat tagttcacaa acagtgcttc
      181 gctgtcgacg atgattccat cttccgcacc tttaagcgcc aggtttgcct cgtagtattg
      241 ctttttatca agcagtttct gcgcatcagc aagtgcatta cgagtctgtt taagcggcat
      301 cagatattgg ttttccatta cgccaacacc ggccaggcga agttcttcca ttgcgccctt
      361 tttatcccct ttcgccattt tttcattggc aatttttatc gcggcttctt tttcgggagt
      421 tgctacataa ctctcagaaa ttccgacaga tgcattaatg acaatatatt ggtcatcatt
      481 gagattggtc tttttacctg gcttagcgaa ctttgcccat tcggtactgt catctgacag
      541 caaagcggaa gcttcattgg tcagttcttt tgccttttct ggatcgccat gaaacagagc
      601 caggcgcgct acctgcacgt cacgcatcgc atataaacct tgttcagaga tacgctctgc
      661 tgctgccgtt ttctgggttt gctgaacttg ctgagtttgc tgttgtgccg ctaccggtgc
      721 attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg tagccataat
      781 taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc atcatattgg
      841 gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta tcttacagtt
      901 gtagttatta tcgcaagaag tattttagtc ttacaattgt aggaatctgc ttcctgtcgc
      961 agagaactat tcttat
//
LOCUS       DAZGLJ010000532          970 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000532 DAZGLJ010000000
VERSION     DAZGLJ010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.545"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..970
                     /locus_tag="ACHK49_005309"
                     /pseudo
     CDS             1..970
                     /locus_tag="ACHK49_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          347 a          179 c          180 g          264 t
ORIGIN      
        1 taacatctct aaggatgaat gggaaaacct ccatgcatta aaagaaaagc gtttaaatgg
       61 aatgcctaaa acaacaccca aaagtgacca ggttattatg ttgcaaaatc aatacgttcg
      121 tgaaatgcga aaatatggcg tacgaggttt acgttatgat gcggcaaaac actcaaaaca
      181 tgaacaaata gaaagatcaa taaccccacc gcttaaaaat tataatgagc ggttacacaa
      241 tactaactta tttaacccaa aatatcataa aaaagccgtt atgaattaca tggaatatct
      301 ggtaacttgt cagttggatg aacaacaaat gtcatcactg ctttatgaaa gagatgattt
      361 aagcgccatt gatttttcat tgctcatgaa aacgataaaa gccttttcat ttggtggaga
      421 tcttcaaacc cttgcatcaa aaccgggttc cacaatctca agtatcccat cagaaagacg
      481 gatattgatt aacattaacc acgattttcc taacaatggt aatcttttca atgactttct
      541 atttaaccat caacaagatg aacaattagc aatggcatat atagccgctc tgccgttcag
      601 caggccttta gtttactggg atggccaagt attaaaatca acgactgaaa ttaaaaatta
      661 tgatgggtcc acgcgtgtcg gcggtgaggc gtagcttaat aaaggttgct ctacctatca
      721 gcagctctac aatgaattcc acgcattata tatagataaa gcaggaatat ggagcgcatt
      781 tgagggtgta tctgcaacta aaaacgttct ggcctttagt cgtggggatt ctgtgaacat
      841 taatcactct cctcatgatg gactagttat aataaataaa ggaaacgaag aagttgaagg
      901 tacctggcct aacaaattgc aacctggaat atacaaaaac atggggagta atagcgttaa
      961 cattattatt
//
LOCUS       DAZGLJ010000533          969 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000533 DAZGLJ010000000
VERSION     DAZGLJ010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.546"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            92..565
                     /locus_tag="ACHK49_005310"
     CDS             92..565
                     /locus_tag="ACHK49_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265745.1"
                     /translation="MDIDTLGGQELRDKIFAGLKIYEGKPFIERFGLFMGKAQLLEFG
                     LKKILVSFPGYNLDEEKLERLTLGQTRVELKKLGLRTDYNAYLKSFKDQRNTMAHEFL
                     ANFAVTQQLLDGAALIRTFERELDHACYAVEQLIILFDFINGAGDVTAWLEPTAP"
BASE COUNT          276 a          199 c          213 g          281 t
ORIGIN      
        1 ctgtccacaa ctaccaatag ctttacataa tgtgtgcatc taacttcaat aagttacttc
       61 attaccaata atgtctaaaa aggaaacgcg catggatatt gatacgctgg gtggccagga
      121 acttcgggat aagatttttg ctgggctcaa aatatacgag ggtaaaccct tcatcgaacg
      181 gttcggtctg tttatgggca aggcacagtt gctagagttt ggcctaaaaa aaattcttgt
      241 ttcttttccg ggatacaacc ttgatgaaga aaaattagag aggctgacat taggccagac
      301 ccgcgttgag cttaaaaagc tggggctgcg cacggactat aatgcttacc tgaagtcatt
      361 caaagatcag cgcaatacga tggctcacga gttccttgct aattttgcgg tcacacagca
      421 gcttttagac ggagctgcac ttattcgcac atttgagcgt gagttagacc atgcctgtta
      481 cgcggtggag cagttgatca tccttttcga cttcatcaac ggtgccggtg acgtaaccgc
      541 ctggcttgag cctactgccc cctgatactt cgtatgcttt tatctctagg ctggacagaa
      601 tagtaattct gtccaggtat gcagaatggc tgtctatata tcttcattag ttcacagtag
      661 gttaccggcg gctttgagcg aacagcggaa cttcgccctt taataaatga taataaattt
      721 tcattatcac tttagttgaa aatggtgtcg aagatgaaga gaaatgtcat attagcctac
      781 ggagtacttg tgaaataaat tcatagcatt ccctaacatc taaacttagg gctcactgaa
      841 cgatggtgaa ccctatgcca ttgggttaat aactattgat aattttgtga agtgcagggt
      901 taattgtcga tgaaatatca aaatcggaat ttctaatcga cccagtgctc catccctctt
      961 tgaaaaaga
//
LOCUS       DAZGLJ010000534          959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000534 DAZGLJ010000000
VERSION     DAZGLJ010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.547"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..882
                     /locus_tag="ACHK49_005311"
     CDS             <1..882
                     /locus_tag="ACHK49_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9265746.1"
                     /translation="FPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVM
                     RYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGSYKGQPVTQLAVM
                     LNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSK
                     QVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAM
                     ACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRE
                     RFISPFEYAKINNPLKQ"
BASE COUNT          267 a          229 c          219 g          244 t
ORIGIN      
        1 ttccccttta tcggcaatcg ggatatcaca acactcaata ccccggacct gcttatccct
       61 gttcgtgctg cagaagctaa acaaatttat gaaatcgcca gtcgtctgca gcaaagaata
      121 tctgccgtaa tgcgttatgc cgtacagtct ggcatcatca gatataatcc tgctctggat
      181 atggctggcg cattgactac ggtaaaacgc cagcatcgcc ccgctcttga tctttctcgc
      241 ctgcctgaac ttttgtcgcg tattggcagt tacaaggggc aacctgtcac ccagcttgcc
      301 gttatgctga atttactggt ttttattcgt tccagtgaac tcagatacgc ccgctggtct
      361 gaaattgata ttgacaatgc catgtggact attccagccg aacgcgaacc tctgccaggc
      421 gtaaaattct cacaccgggg atccaagatg cgaacaccac atcttgtgcc actcagcaaa
      481 caggttgtag ccatactggc agaacttcag acatgggctg gtgaaaatgg tctgatcttt
      541 acgggagcac atgacccacg taaaccaatc agtgaaaaca ccgtaaataa agccctgagg
      601 gttatggggt atgacacaac ccaggatgtc tgtggtcatg gattccgggc gatggcatgt
      661 agtgcgttaa tagaatcagg tttgtggtcc cgcgatgcag ttgaacgtca gatgagccat
      721 caggaacgta acggtgtacg tgctgcttac attcataaag cagaacatct ggaagaacgt
      781 cgactgatgc tacagtggtg ggccgatttt ctggatgcaa acagagaaag atttatcagt
      841 ccatttgaat atgcaaagat taataatcca ttaaaacagt aatcatcccg ggcaaatgcc
      901 cgggaaccta ctccaggatt attttctttg ttaaaaaaac agacaaaacg gtattaact
//
LOCUS       DAZGLJ010000535          955 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000535 DAZGLJ010000000
VERSION     DAZGLJ010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.548"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..120
                     /locus_tag="ACHK49_005312"
     CDS             <1..120
                     /locus_tag="ACHK49_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO9265747.1"
                     /translation="NPMPVPYGEMLVGSRRISQDISTRDEGGGGKVVVIGRQA"
     gene            complement(311..838)
                     /gene="sodC"
                     /locus_tag="ACHK49_005313"
     CDS             complement(311..838)
                     /gene="sodC"
                     /locus_tag="ACHK49_005313"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO9265748.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
BASE COUNT          230 a          229 c          261 g          235 t
ORIGIN      
        1 aacccgatgc cggtgcctta cggggaaatg ctggttggct cccgccgtat atcccaggac
       61 atcagtaccc gtgatgaagg cgggggcgga aaggtcgtgg ttatcgggcg gcaggcataa
      121 aagcgaaaaa atcccgcagt gctcacggac aggaactgcg ggagcgttac gaagattgag
      181 tgtaaggaat tattcttatg tcacgacaaa aacattaact cagagaggga ggatgtgccg
      241 ttcttttcag ggagaaagga tttatcgtcc tgaggaataa aggtaagggg cccgcccctt
      301 acctgactga ttattgaatg atgccgcagg ccattctcgc accaccaccg cccaggggct
      361 ccggatggtc atgatggtta tcaccgccag catgaagcat gagagaacgc cctttaatct
      421 cttttaatga gttcagtctc ggggccagga ccgggtagtt cgcttttccg tcatgcgtca
      481 cgaacagcgc agggaggtcg cccaggtgtc catccggaga ccaggggcca agatgtttgc
      541 cggtgttttt cgggtcaaag tgaccgccag ccgataatgc tgcgaccggt tttccgtctt
      601 tcagtgccgg ggcgcaattt cctttttcgt gcacatgaaa accatgaatg ccttcagaca
      661 gagagtgaag ggctggtgtg aacagcagac cgtagggggt ctcctgaatg gttatttttc
      721 caatgctgac ttcttttccg tcagcactga caaggttcat tgggacttcc tgttctgctg
      781 cgtatccgca tgatgctgct gtcagcatgg caatggcagc aatgatttta catttcataa
      841 aaccctcatt aattccgtta acagactgag cttgctggtt acagggtaac aaacagcgtt
      901 ctgatgatat cgcgcaatag ctgtgcaata tcctatcact gcgattaata atacc
//
LOCUS       DAZGLJ010000536          954 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000536 DAZGLJ010000000
VERSION     DAZGLJ010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.549"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            55..>954
                     /locus_tag="ACHK49_005314"
     CDS             55..>954
                     /locus_tag="ACHK49_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFO9265749.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATIREGGLITAENTIIGGNATGV
                     GTLNVQDQDSVITVRRLYNGYFGN"
BASE COUNT          249 a          173 c          265 g          267 t
ORIGIN      
        1 tgtctgacga ctcatctctt tcgtggctgt attttttgta tatggagcgt ttatatgaat
       61 agaatctatc gcgtgatatg gaattgcact ctacaggtat ttcaggcctg ctcggaatta
      121 actcgcaggg caggtaaaac atcgacggtt aatttgcgta aatcctctgg actgacaacg
      181 aaattcagta gattgacgct gggtgttttg ctggcactaa gcggttcagc gtctggtgca
      241 agtctggaag ttgataatga tcagattacc aatattgata ctgatgttgc ttatgatgcc
      301 tacctggttg gctggtatgg cactggagtg cttaatattt tggctggcgg taatgcctcc
      361 ttaaccacta ttactaccag cgtcattggc gctaatgagg actcagaggg caccgttaat
      421 gttttgggtg gcacctggcg attgtatgat agcggaaata atgcaaggcc tttaaatgtg
      481 ggtcaatccg gaacggggac gctgaatatt aaacagaagg gtcacgtcga tggaggctat
      541 ttaagattag gttcttcgac aggaggcgtc gggacggtca atgttgaggg agaggactct
      601 gttctgacga ccgaattatt cgaaataggg agttacggta cgggctcatt aaatattacg
      661 gataagggtt acgtcacgag ttcaatcgtc gccattttag gctatcaggc gggcagtaat
      721 ggccaggttg tcgttgaaaa gggtggcgag tggctaataa aaaataacga ttcctcaatt
      781 gaatttcaaa ttggtaatca aggaactggg gaggcgacta ttcgcgaggg tggactgatt
      841 acggctgaaa atacgattat cggtggcaat gccaccggtg tcggaaccct gaatgtgcag
      901 gatcaagact ctgtcatcac ggtacgcaga ctctataatg gatatttcgg taat
//
LOCUS       DAZGLJ010000537          953 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000537 DAZGLJ010000000
VERSION     DAZGLJ010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.550"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..608
                     /locus_tag="ACHK49_005315"
     CDS             <1..608
                     /locus_tag="ACHK49_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HFO9265750.1"
                     /translation="SPMTMVGRSLAQAATQTLRPAVTRAATQAGVGASGMKFVPMQSN
                     FMINHGKLTNQLLQAVAKQTRSGDTQQWFKQEQITYISRTVNRAIDDYCMSNNSVIGK
                     ETKSHIFKAVESAIKQPLDMNCAQTSIGHFLQSNKYFNKKVDEQCGKGVDPITRFNTQ
                     TRMIEQVSQEIFEQKFGGLKVSDIKALTQSTIAQHVQDTRL"
BASE COUNT          299 a          160 c          199 g          295 t
ORIGIN      
        1 ttagtccaat gacaatggta ggtagatcgt tagctcaggc ggctacacaa actcttagac
       61 ctgcggtcac cagagctgcg acacaagcgg gggtgggagc cagtggcatg aagttcgtgc
      121 ccatgcagtc gaactttatg attaatcatg gcaaactgac taaccagcta cttcaggccg
      181 tggccaaaca aacccgtagc ggtgatactc agcaatggtt caaacaggag caaattactt
      241 atatatccag aacagtaaac agagctattg atgactattg catgagtaat aattcggtaa
      301 ttggtaagga aaccaaaagc cacattttca aggctgttga aagcgcgata aagcagcctc
      361 tcgatatgaa ttgtgcacaa acgtcgattg gccatttctt acagtccaac aaatacttta
      421 ataaaaaagt tgatgagcag tgtggcaaag gggtggatcc aattacacgc tttaacaccc
      481 agaccaggat gatagaacaa gtctcacaag aaatctttga acagaaattt ggtggtttga
      541 aagttagtga tatcaaagct ctgactcaga gcacgattgc acagcatgta caggatactc
      601 ggttgtagga ataacctgtt atatatgcgt gttgggcata agttaagctt cctggtgtat
      661 agcgtagtct ttgctgatgt aagaaaaggc tatatacgga aacttgatat actataaact
      721 tcattattta ttatattgaa aaagttattt aattgatttg ttttgtaatt tcaatgcttc
      781 aattggataa gcataaaata tagttttatt tatagtgtgt tttattaaag tttcacgtgg
      841 catttatttt tatatcgcat gattattttc atttaattac actgatgtgt atttttctgc
      901 attattttct tgcatcaaaa atcagactgt gatttatttg gtcgatgtta tgt
//
LOCUS       DAZGLJ010000538          952 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000538 DAZGLJ010000000
VERSION     DAZGLJ010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.551"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            82..453
                     /gene="ler"
                     /locus_tag="ACHK49_005316"
     CDS             82..453
                     /gene="ler"
                     /locus_tag="ACHK49_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFO9265751.1"
                     /translation="MNMETNSHTTSPYIQLIEQIEVLQQEAKRLREQEIQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEDEIIPLKNI"
     gene            468..686
                     /gene="escE"
                     /locus_tag="ACHK49_005317"
     CDS             468..686
                     /gene="escE"
                     /locus_tag="ACHK49_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscE family type III secretion system
                     co-chaperone EscE"
                     /protein_id="HFO9265752.1"
                     /translation="MITITELEDEIIKNKEAANIFIEKINDKKNEIHEKMKHPLDKVT
                     YNEAKELLIACDAAIRIIEIMLIRINNK"
     gene            699..>952
                     /locus_tag="ACHK49_005318"
     CDS             699..>952
                     /locus_tag="ACHK49_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system filament chaperone"
                     /protein_id="HFO9265753.1"
                     /translation="MSQTKNRELLDKKIRSEIEVIKKIIAEFDVVKENVNALSEKAKT
                     DPQAAEKLNKLIEGYTYGEERKLYDSALSKIEKLIETMSPP"
BASE COUNT          392 a          143 c          192 g          225 t
ORIGIN      
        1 ttcgattaat tgttgatcct tcctgataag gataagattg ctaatagctt aatatattaa
       61 agcatgcgga gattatttat tatgaatatg gaaactaatt cgcacacaac aagcccatac
      121 attcagctta ttgagcaaat tgaagtgtta caacaggaag caaagcgact gcgagagcag
      181 gaaattcaaa gtgtaattga gtcgattcaa aagcagatta cttattacaa tataacccta
      241 caagagctgg gatatactaa tgtgcctgat gatggccttg ctcgccggaa ctcatcgaaa
      301 ggagtttatt atcgcaatga agaagggcag acctggtcgg gagttggccg acagccacgc
      361 tggcttaaag aagcactgtt gaatggaatg aagaaagaag attttcttgt gaaggacacc
      421 gaagacgaaa taataccgct gaaaaatatt taacatgaaa tcattaaatg ataacgataa
      481 ctgaactgga agacgaaata ataaaaaata aagaagccgc aaatattttc attgaaaaaa
      541 taaacgacaa aaagaacgaa atccatgaaa aaatgaaaca ccccttggat aaagttacct
      601 acaatgaagc aaaggaactg ctcattgcct gcgatgcggc aatcaggatc atagagataa
      661 tgctaatcag aattaataat aaatagagga cgagtattgt gagccaaaca aaaaatagag
      721 aactgttaga taaaaaaata agatcagaaa ttgaggtgat aaaaaaaata atcgccgaat
      781 ttgatgtcgt aaaagaaaac gttaatgctt taagtgaaaa agcaaaaaca gatcctcagg
      841 ctgcagaaaa attaaacaaa ttaattgagg ggtatacata tggtgaagag cgaaaattat
      901 atgattctgc cttaagtaag attgaaaaac taatagagac aatgagtcct cc
//
LOCUS       DAZGLJ010000539          950 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000539 DAZGLJ010000000
VERSION     DAZGLJ010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.552"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>950
                     /locus_tag="ACHK49_005319"
     CDS             <1..>950
                     /locus_tag="ACHK49_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9265754.1"
                     /translation="EAYRGSPQLMVERLNALTENQRQVADARIAIVSEVLKVAQQPGF
                     SCAKAIRFIVDNLARSQLDERIVAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAA
                     ERLLLLAPGKRQEIKAEDINWLPEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYM
                     LDIMPSYDTIRRAMKKLPEVVKQKGRVTGSEYRQLEGFTRRDWSKMPVNYVWIGDGHG
                     MKLKCAHPVHGRPFAPEVTFVIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPF
                     LYYSDNGSGETADILDKEVVGILPRLGINHPTGIAGNPQGR"
BASE COUNT          231 a          247 c          281 g          191 t
ORIGIN      
        1 gaggcatatc gcggatcacc acaactgatg gtcgaacgcc ttaatgccct gactgaaaac
       61 cagcgccagg tggctgatgc acgaatcgcg atcgtcagcg aagttctgaa agtcgcgcaa
      121 caacctggtt tcagctgtgc gaaggctatc cggtttatcg ttgacaacct ggcacgttcg
      181 cagctggacg agcgcattgt ggcaatggtt gagacggcga acgccaaaaa gggaaacagc
      241 cgcgcgttaa gtgaaatcac gctgaaacgc tggattgcgg cctttaacaa ggcgcagaac
      301 gccgctgaac gcctgctttt actggcaccg ggtaagcgcc aggaaataaa agccgaagat
      361 attaactggc tgcccgaatt tctggcgcag tatcgccagt caaacggccg accaatgacc
      421 gaggcttacg aggattttgt cgctgaatgg cagcaccggc acgctgatga gccttatatg
      481 ctcgatatca tgccgtctta tgacaccatt cgccgcgcaa tgaagaaact gccggaagtg
      541 gtgaaacaaa aaggccgggt gaccggcagt gaataccgcc agcttgaggg attcacgcgc
      601 cgcgactggt caaaaatgcc ggtgaattat gtctggattg gtgacggtca cggcatgaag
      661 ctgaaatgcg cacacccggt tcacggtcgg ccatttgcac cggaagtgac ctttgttatc
      721 gacggtggca cgcgctttgt ggtgggctgg agccttgacc tggctgaaaa tgttttcgcc
      781 gtagccggtg ccatacagca cggcattcgc catcacggca aaccgtttct gtattactcg
      841 gataacggct ccggggaaac cgccgacatc ctggataagg aggttgtggg gatactgccg
      901 cgactgggga ttaatcaccc gaccgggatt gccggtaatc cgcagggacg
//
LOCUS       DAZGLJ010000540          950 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000540 DAZGLJ010000000
VERSION     DAZGLJ010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.553"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            400..>950
                     /gene="nleG7"
                     /locus_tag="ACHK49_005320"
     CDS             400..>950
                     /gene="nleG7"
                     /locus_tag="ACHK49_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HFO9265755.1"
                     /translation="MPIFLNFTAGSILPENELASLRYIVQQNQNDTVIIKERYKMDIR
                     YIESVNGFTVNPVCSNHFSIFMARQNTIARNLEQQINNGRSFAQISQDFMLQLSSNIG
                     WKKGAENALKNKIHSHSFVVNPDEFSCDTQFLKCPITLCVPEKGVFVKNALNSNICTL
                     YDKSAFMNLTREHLPHPLSREKIV"
BASE COUNT          315 a          204 c          141 g          290 t
ORIGIN      
        1 gtcattgcaa tcgacctgcg ctatcgctgc cgcagttctt ccagtttctg catcgataac
       61 tccatcttct gagcaataaa cctcacctat gcaatatttt atgtcaatta tttcaattgc
      121 attaatatct tgaatggatt acatagagtt aatgtatcca gtactcccta ttctcttgca
      181 tataatacat gttgcaactt acatctcagc gctatgaaaa aacaccacct ctctctttat
      241 gaaattctgg atttaccaag cgctaattta tcatttcaat ctacctttaa gcattgcatt
      301 tatctcccta cgaggtcata ctttaggaag ttaaatatga atgataatat acctacagcg
      361 cgaaatcaca aacaatcgac ttgtattaca gaaaaaaaca tgcctatctt tttaaacttc
      421 acagcaggca gtatcttacc tgagaatgag ctagcatctt tacgttatat tgtgcagcaa
      481 aatcaaaatg atactgtaat cataaaagaa cgttataaaa tggatatccg ttatatcgaa
      541 tcagtcaatg gttttacagt aaatcctgta tgcagtaatc atttctccat atttatggcg
      601 agacaaaaca ctattgctcg caacctggaa cagcagatca acaacggacg aagttttgca
      661 caaatatctc aggattttat gcttcaatta tcttcaaata taggatggaa aaaaggggcc
      721 gaaaacgccc ttaaaaataa aatccattct cattcatttg ttgtaaatcc tgatgaattc
      781 tcttgcgaca cacaatttct taagtgccca ataacattat gcgttccaga aaaaggggtt
      841 tttgtcaaga acgcactgaa ctccaacata tgcactcttt atgataagtc tgcgttcatg
      901 aatctcacaa gagaacatct accccaccct ctcagcaggg aaaagatagt
//
LOCUS       DAZGLJ010000541          949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000541 DAZGLJ010000000
VERSION     DAZGLJ010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.554"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(39..635)
                     /gene="fimE"
                     /locus_tag="ACHK49_005321"
     CDS             complement(39..635)
                     /gene="fimE"
                     /locus_tag="ACHK49_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO9265756.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          301 a          199 c          174 g          275 t
ORIGIN      
        1 atttatttgt tttttaactt tattatcaat gagttaaatc aaacctcttc tctttttaat
       61 ttttcgttta tgagattatt tctttcccat aatccggcaa aacgagcagc attactggcg
      121 gtataacgta cagtatggcg aatatttcga tgcccgagat aatcctgaat taaacgagta
      181 tctgcaccac gctccgccag ttcataaccg caagcatgcc ttaacatatg aggatgagtc
      241 tgcgttacgg ttccagcttc aataccggca tcgcgaataa tgcgataggc ctgctggcga
      301 gaaagccgac tcccacggcg agaaataaat atagcgtcgg tccggtcagc gcctttccag
      361 ttagcacgtt cctgggtcca gcgttccacg gcttcacgct catcaaaacg taacgggtga
      421 acggtagaaa atccgttctt cagtcggcga atatttattc gaccttcatt aaggtcaaga
      481 tcctgataat gcagatcaag cagttcacta atacgcatcc catgccgata tgccaacaga
      541 ataagacaat aatctctggc tcccgttgcc ccgtaacaaa ccgcctgcat catggcctga
      601 acttctttac cggtaagata acgacgttta ctcacaatag tagtactcct gactgagata
      661 tatttgaatg aatacctata ggaaacctca atcggtcaaa attagcccaa tagataaaaa
      721 gataaccaca cacgaagtga tgtggctatc agtcaattac attaaatatc atacaaaaat
      781 aaaatatcac tgatgtgata acatctattt tcattgattt gattcaatat aacaatagat
      841 tgcagtggat taacttccag ttgcttaaaa aataaagaaa aaagtgcgga aacaggagaa
      901 tttaagtgat gtgtaatttt atgaatgatt ttgtttattt atttttaag
//
LOCUS       DAZGLJ010000542          949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000542 DAZGLJ010000000
VERSION     DAZGLJ010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.555"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            175..861
                     /locus_tag="ACHK49_005322"
     CDS             175..861
                     /locus_tag="ACHK49_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFO9265757.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
BASE COUNT          268 a          181 c          223 g          277 t
ORIGIN      
        1 gtgtgttgtt atttttttgt ggaaggcaag ccgcaatggc taaataggga tggttgtaat
       61 aatcaaaaca tgatactaac tatatattga tcgttgcatt gttggcatgt aacacgctgc
      121 agcgagtaac attggtaatg aaatataatc aacatggata ggtgaaaatt aattatgagc
      181 tttatggctt ttgtaacttg tttgacaatt gcatttatcg ccggttttgc tgttgccaga
      241 aaattgacac tccgaaaagt tgcctcatcg gtaatcatcg ctaaatcgca acccgtccct
      301 tcatctgcaa cttcccctgc cgctaaggcg tcgacaagga gctatatacc gcagaaaacc
      361 tatcgtgact ctcgttggca agagcgagat gagttatttc agggaatggt tgccgaaggt
      421 attttattgg aaagaaaaga gtggttaatc acgcgttgtg aaaaaatgta tttcgataat
      481 ctggaaaaat ggtttggaca ttattgtcat atccattgcc aggtctctct ggggcgcttg
      541 atcaactttc cagaacaacc tgagtttagc gaagaagaaa gaaagcgttt ctttgcaatt
      601 tataatggca tggcaatgga ttatgttctg gtttcaaaga agaacaataa aatagtctgt
      661 gttattgagc tggatgaccc gagtcatgaa agggcggatc gcattattcg tgaccgttgg
      721 ttggatacaa ttatgactct tgcacgggtg ccttttgtac gagcttctgt cactaaattg
      781 gaggaaattc ctccggtctg ggagactcgt gacaaaattc gcccattagt cgcggcgaca
      841 aagagtgaac cggtgcatta actaagcatc ccggtcgggg cttctcatcc ccccggtgtg
      901 tgcaatatac gaaaaaaagc ccgtaccttc gtacgagctc ttctttaaa
//
LOCUS       DAZGLJ010000543          948 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000543 DAZGLJ010000000
VERSION     DAZGLJ010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.556"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            62..487
                     /gene="tnpA"
                     /locus_tag="ACHK49_005323"
                     /pseudo
     CDS             62..487
                     /gene="tnpA"
                     /locus_tag="ACHK49_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(551..844)
                     /locus_tag="ACHK49_005324"
                     /pseudo
     CDS             complement(551..844)
                     /locus_tag="ACHK49_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
BASE COUNT          275 a          212 c          197 g          264 t
ORIGIN      
        1 ctcctgttga ccaccgggtc ttaactggaa tttaaatgcc tgtagtcgtt ccacaggtca
       61 aattatattt tggtctgtga aaaaagaaac cgatatccgg cgtggaaggc attgtgtttt
      121 cctgatgcat gttcacctgg tctttgtcac caaataccga cgccagattt ttgaccatga
      181 cgcgacagaa aaactacgca cttacttttc aaatgtatgt gctgattttg aagctgaact
      241 ggttgaaatg gatggcgaac cagatcacgt ccatttgtta attagctatc ctcccaaact
      301 ggcgatatcc agtctggtaa acagcctcaa aggcgtatcg ggtaggttac tgcgacgaga
      361 tcgaccagat attgcagtca ggtattacta caaaggcgtt ttgtggagtc ctggctattt
      421 tgccaatagc tgcggaggtg cgccaatatc cgtcatccgc caatacattg aacaacagca
      481 aacacctggt caggtggaaa accgcgcctt atatccccgc cctgaaggac ggggttttac
      541 ggcgcaccag ataaaccagg ccaagcgtat gctggttgat caaataatat cccccaagcg
      601 cagcaatatt aatattcacc tgcaatgggt cgatacctgg cttaaaaata ttgtatttct
      661 tcccttcatc caataaggat tccattaact gcaagtgcgc atggttaatt tctaagagac
      721 gctgtgattt tgcataatgc acacctttac tttgattttc actatgcaca atttttaaaa
      781 accaagggtt agcaatataa taaccccatg taaaatcaat aattttctca atggcttttt
      841 gtggctggtt aaccagatga ttaacaaacg caccgatacc aacagcgtag aataattaca
      901 taccgcacaa caagaatgta tggtggaagg cgtggaagtc attttctc
//
LOCUS       DAZGLJ010000544          939 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000544 DAZGLJ010000000
VERSION     DAZGLJ010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.557"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..182
                     /locus_tag="ACHK49_005325"
     CDS             <1..182
                     /locus_tag="ACHK49_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265758.1"
                     /translation="RKFKIIIETGIAGGDFEDEFEVDDDATPDEIHDEAKDIFFNYCN
                     YSYHEIKDEEEEQNG"
     gene            175..351
                     /locus_tag="ACHK49_005326"
     CDS             175..351
                     /locus_tag="ACHK49_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265759.1"
                     /translation="MADFGSTKYSVSFEEWHELLMEYAELRGGSAADAEAWRDDYEAG
                     KNPVEAYCDEWGDE"
     gene            348..>939
                     /locus_tag="ACHK49_005327"
     CDS             348..>939
                     /locus_tag="ACHK49_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO9265760.1"
                     /translation="MSEIDYQALREAAEKATKGCYIVGHTSGNQHGNITGVFVCQKWK
                     GEPGGVIAECHVNCLVETDVQAYANAEFIAAFNPNVALALLDEREAQNKRIAELKANL
                     VALAAENAVIKSAIPEPRDIEDDNDNMDDVSLAEDFGFNHAIERMRRRIPETPATDAF
                     LAEVRAGLFNELCAAFVRYEKVAGLDDSDTVTLKEAT"
BASE COUNT          295 a          155 c          264 g          225 t
ORIGIN      
        1 tgcgaaagtt taaaataatt attgaaacgg gaatagccgg tggagatttc gaggatgaat
       61 tcgaagtgga tgatgatgcg acgcctgatg aaatacatga cgaagcaaaa gatattttct
      121 ttaactactg caattattca tatcacgaaa taaaagacga agaggaagaa caaaatggct
      181 gattttggtt caactaaata cagcgtcagt tttgaagaat ggcatgaact gttaatggaa
      241 tatgcagagt tacgtggtgg cagtgctgct gatgctgaag catggcgtga tgattatgaa
      301 gcaggaaaaa atccggtcga agcatattgt gatgagtggg gcgatgaatg agcgagattg
      361 actatcaggc gctgcgtgaa gcagcagaga aagcaactaa aggatgctac atcgtagggc
      421 atacatcggg caatcagcat gggaatataa caggagtttt tgtttgtcaa aaatggaaag
      481 gagagcccgg tggcgtaatt gcagaatgtc atgttaactg cctggttgaa acagatgttc
      541 aggcttatgc aaacgctgaa tttattgctg cttttaatcc aaatgttgcg ctggcactac
      601 tggatgaacg ggaagcccaa aacaaacgaa ttgcagagct gaaagctaat cttgtggcgc
      661 tggcggcgga gaatgcggta ataaagtctg caattccaga accacgggat attgaggacg
      721 acaatgacaa tatggatgac gtatctcttg ctgaagattt cgggttcaat catgcaatag
      781 aacggatgag gagacggatt cctgaaactc cggccaccga cgctttcctg gctgaagtac
      841 gggcggggtt gtttaacgag ctttgcgcgg cgtttgtcag gtacgaaaaa gttgcaggac
      901 tggatgatag cgatacagta acgcttaagg aagcgacag
//
LOCUS       DAZGLJ010000545          933 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000545 DAZGLJ010000000
VERSION     DAZGLJ010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.558"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            30..>933
                     /gene="yfdF"
                     /locus_tag="ACHK49_005328"
     CDS             30..>933
                     /gene="yfdF"
                     /locus_tag="ACHK49_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFO9265761.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGNSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSEKARNASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISNLF"
BASE COUNT          359 a          162 c          166 g          246 t
ORIGIN      
        1 taacaaatca atcaaaatgg aataaaatca tgctaccatc tatttcaatc aacaatacca
       61 gcgcaactta cccagaatcc attaatgaaa ataacaatga tgaagttaat ggattagtac
      121 aagagctcaa aaaccttttt aatggtaagg aaggaataag cacctgtatt aaacatctac
      181 ttgagcttat aaaaaacgcc atacgagtaa acgacgatcc ttatagattt aatattaata
      241 attcctcagt tacttatatt gatattggct ccaatgatac agaccatatt actattggta
      301 tcgacaacca agaaccaata gaattacctg cgaactataa agacaaagaa ctcgtccgta
      361 ctatcattaa tgacaacata gttgagaaga ctcatgatat caataacaag gaaatgatct
      421 tcagcgcatt aaaagaaata tatgatggag atcctggttt tatcttcgat aagatatcac
      481 acaaactcag acatacggta acggagtttg atgagagcgg gaatagcgaa ccaacggact
      541 tatttacctg gtacggtaaa gataaaaaag gcgactctct cgctattgta attaaaaata
      601 aaaacggaaa tgattactta tctctcggtt actacgatca ggacgactac cacattcaaa
      661 gaggaattcg tattaatggt gatagtctca cccaatattg tagtgaaaaa gccaggaatg
      721 cttcagcgtg gtttgaaagc agtaaagcta tcatggcaga atcatttgca acaggttccg
      781 atcatcaggt tgtaaacgag ctcaacgggg aaagactgag agaacctaac gaagttttta
      841 aacgtttagg tcgagcaata agatataatt ttcaagtgga cgatgcaaaa tttagacgcg
      901 acaatgtaaa agaaataatt tctaatttat tcg
//
LOCUS       DAZGLJ010000546          932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000546 DAZGLJ010000000
VERSION     DAZGLJ010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.559"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..467
                     /locus_tag="ACHK49_005329"
     CDS             <1..467
                     /locus_tag="ACHK49_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9265762.1"
                     /translation="PQILDQFLDEKEGNHTMAYRDGSGIWTICRGATVVDGKTVFPNM
                     KLSKEKCDQVNAIERDKALAWVESNIKVPLTEPQKAGIASFCPYNIGPGKCFPSTFYK
                     RLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQVIRRDQESALTCWGIEQ"
     gene            464..>932
                     /locus_tag="ACHK49_005330"
     CDS             464..>932
                     /locus_tag="ACHK49_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFO9265763.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYE"
BASE COUNT          266 a          200 c          248 g          218 t
ORIGIN      
        1 ctcctcagat acttgaccag tttctggacg aaaaagaagg taaccacaca atggcatacc
       61 gcgatggttc tggcatatgg accatctgtc ggggtgccac agtggtggat ggaaaaaccg
      121 tttttcccaa tatgaaactg tcgaaggaaa aatgcgacca ggtcaacgcc attgagcgtg
      181 ataaggcgct ggcatgggtg gagagcaata ttaaagtacc actgaccgaa ccacaaaaag
      241 cgggtatcgc gtcattttgt ccctataaca ttggccccgg taagtgtttc ccgtcgacgt
      301 tttataagcg gctgaatgct ggtgatcgta aaggtgcatg cgaagcgatt cgctggtgga
      361 ttaaggatgg cggacgcgat tgccgcattc gttcaaataa ctgttacggt caggttattc
      421 gtcgtgacca ggagagcgca ttaacctgct gggggataga acagtgaatc agatattcat
      481 ggtgattttt ctcgtgttgt caggatttat cgtcggaaat gtctggagcg acagaggatg
      541 gcaaaaaaaa tgggcggaac gtgatgctgc cgcattatca caagaggtaa atgctcaatt
      601 tgctgctcga ataattgaac aggggcgaac tatagcccgt gatgaggctg ttaaagatgc
      661 acaacagaaa tctgctgaaa tttctgccag ggctgcttat ctgtctgata gtgttaacca
      721 gttgcgtgcc gaagcaaaaa aatatgccat acgccttgac gcagcgaagc ataccgcaga
      781 tcttgccgct gccgtcagag gcaaaacaac caaaaccgcc gaaggaatgc tcaccaacat
      841 gctcggagat attgcagcag aagctcagct ttatgctgaa attgctgacg aacgctacat
      901 cgcaggagtg acttgtcaac agatctatga at
//
LOCUS       DAZGLJ010000547          931 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000547 DAZGLJ010000000
VERSION     DAZGLJ010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.560"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..152
                     /locus_tag="ACHK49_005331"
     CDS             <1..152
                     /locus_tag="ACHK49_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO9265764.1"
                     /translation="AGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQVSRRDQESALAC
                     WGIDR"
     gene            423..>931
                     /locus_tag="ACHK49_005332"
     CDS             423..>931
                     /locus_tag="ACHK49_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO9265765.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEA"
BASE COUNT          266 a          195 c          245 g          225 t
ORIGIN      
        1 atgcaggaga tcgaaaaggt gcctgcgaag ctattcgctg gtggattaag gacggtggca
       61 gggactgccg tattcgctca aataactgtt acggtcaggt atcccgtcgt gaccaggaga
      121 gcgcgctggc gtgctgggga atcgacagat aagcagaata ttttgctgaa aaatgaggtt
      181 tgcttacatg gatggataac acgaaatcct gcaaattggc aaaatgtaag tgaataaagt
      241 caaaacagtt gtttaacact caggcaccgt aatgatgcct ttgtcatttc tgcgcatctc
      301 acgcgcatct cacaacacag aacctttcag gatgaccctt gaggataccg gtttggctgt
      361 cggtgccttt ctgtgggctg gattcctgtg agacaaggtt catcactaaa aggaaataac
      421 cgatgaatat gatggccgtg ccgtttcacg gcaactctct ttatgtagtt aaccataatg
      481 gcgaaccata cgttcccatg aaacctgtcg ttgcggggat ggggctggcc tggcaatcac
      541 agttggctaa gttaagacag cgttttgcgt caactataac ggaaatcgtt atggttgctg
      601 aggatgggaa acaacgcaat atggtgtcca tgccacttcg aaaacttgcc ggctggctac
      661 aaaccattaa tcccaacaaa gtaaaacccg aaatccgcga taaggtcatc cggtatcagg
      721 aagagtgcga cgatgttctt tacgagtact ggacgaaggg ttttgtcgtt aatccccgta
      781 aaatgagcgt gatggaagaa ctcaaccagg cttgtgctga catgaaacgg gataaaaaca
      841 ttgccagtgt gtttgctacc gggctgaatg agtggaaaca ggttaaagcc gcgcatgtat
      901 caaaaatccg tacgctggta aatgaagcga a
//
LOCUS       DAZGLJ010000548          929 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000548 DAZGLJ010000000
VERSION     DAZGLJ010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.561"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..531
                     /locus_tag="ACHK49_005333"
     CDS             <1..531
                     /locus_tag="ACHK49_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9265766.1"
                     /translation="VSHIPAPRDGRDYDPEVLKQAVLEAVNALPAPQDGRDATALEVL
                     PAIDDQKSFPRGTYATHLGGLWRAYEKTHGMRGWECLVDGVADIDVSMTDERLFSVVI
                     RQSSGQCTEKTFSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTGDKPGEAHSS
                     GWTLAAKRGRDAGGGK"
     gene            528..854
                     /locus_tag="ACHK49_005334"
     CDS             528..854
                     /locus_tag="ACHK49_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO9265767.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            865..>929
                     /locus_tag="ACHK49_005335"
     CDS             865..>929
                     /locus_tag="ACHK49_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265768.1"
                     /translation="MAISAGRLTQMISVLNPVLTR"
BASE COUNT          201 a          223 c          312 g          193 t
ORIGIN      
        1 gtaagccata tccctgctcc gcgtgatggt cgtgattacg atccggaagt actgaagcag
       61 gcggttctgg aggcggtgaa tgcccttccg gctccgcagg acgggcgtga tgccacggca
      121 ctggaagtgc tccccgctat tgacgatcaa aaatcctttc cccggggcac gtatgccaca
      181 cacctgggtg gactctggcg ggcgtatgaa aaaacgcacg ggatgcgggg atgggaatgc
      241 ctggttgacg gggtggcgga tatcgacgtc agcatgacgg atgagcggtt gttctctgtg
      301 gtgatccggc agagcagtgg ccagtgtacg gaaaaaacat tttccctgcc ggtgatgctc
      361 taccgcggtg tgttcagagc cggtgaaacc taccaccccg gcgatacggt gacgtggggg
      421 ggctcgctgt ggcactgcaa cagtatgacc ggtgataaac ccggagaagc tcattcatca
      481 ggctggaccc tggctgcaaa acgtggacgg gatgcaggag gcggaaaatg acggcattac
      541 tgacactgga agagatcaag gcacatctgc gtgttgacca tgacgcggat gatgacatgc
      601 tgatggacaa ggttcgtcag gctaccgccg tgctgctggc ctacattcag ggcagccggg
      661 ataaagtgat tcgtgaggac ggtgagctga tcccgggcga ggcattaacc cggatgaagg
      721 gggctgccat gcgactgacc gggatgctgt accggaatcc ggatcttgct gagcgggaag
      781 acctcgtgca gggggaactg ccgttttctg tgtccgtgct gatttacgat ttgcgttgtc
      841 cgacggtgtt atgaggaggg aggaatggca atatctgcag gtcgtctgac acagatgata
      901 agtgttctga acccggtgtt aacccgtaa
//
LOCUS       DAZGLJ010000549          925 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000549 DAZGLJ010000000
VERSION     DAZGLJ010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.562"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..224
                     /locus_tag="ACHK49_005336"
     CDS             <1..224
                     /locus_tag="ACHK49_005336"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265769.1"
                     /translation="QRYDDSGVICTGGPGTLSGIATTKNSKSKGNGAGGVGGNRLIFR
                     YYRPVSAEGGSYLSGLRIPLDSITDWRNT"
     gene            complement(337..533)
                     /locus_tag="ACHK49_005337"
                     /pseudo
     CDS             complement(337..533)
                     /locus_tag="ACHK49_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            709..>925
                     /locus_tag="ACHK49_005338"
     CDS             709..>925
                     /locus_tag="ACHK49_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO9265770.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKR"
BASE COUNT          245 a          244 c          233 g          203 t
ORIGIN      
        1 agcagcgcta cgatgactcc ggtgttatct gcaccggagg accgggaaca ttaagcggta
       61 tagcgacaac aaagaattcg aaatcaaaag ggaatggtgc cggaggcgtg ggcggtaata
      121 ggctgatttt caggtattac cggcctgtat ctgctgaagg gggctcgtat ttgtcggggc
      181 tgcgtattcc gctcgattcg atcacggatt ggcggaatac ctaactgtga ttgattcagg
      241 taccattttg cagggatccg gtgcgttact gtctgacaca acaaacgcag cgggtcgctc
      301 tgctccgccg attcctgata atccctcccc agtaacctac tgcaacagta caaatacact
      361 gacgcctgaa acggtaacca gaatcagcac cagcagtaac tcagtaaaca gagcagacga
      421 tcggtcttcc ggtaataacc ggaaggaacg acagcgccac aatacaggca acacactgta
      481 ttatgagtaa tcgaattacc gcaatccccg catttctttt tgcgtatcac gatagctccc
      541 tcgccacgcc ttatccgtaa ccggtttttt acattaaaaa aaacaacgcc gggaattcaa
      601 tcgtcagtcc ggagacgacc gttcgggtta tcacagagag cctgagcagg ggggctgcgc
      661 attttttacg atgtggctat tccgtatgaa ccatacggag atataaccat gacctacaaa
      721 tacaacccct tctggcagca acgtattcgt gagacggtgc ggcacgcact gaatgttcat
      781 ccccgcctga cggcattgcg ggttgacctg cgtttcccgg atgtaccggc agcaacggac
      841 gcagctgtga tatcccgctt catcaatgcc ctgaaagccc gaatcgacgc ttaccagaaa
      901 cgtaagcatc gggaaggtaa acgcg
//
LOCUS       DAZGLJ010000550          923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000550 DAZGLJ010000000
VERSION     DAZGLJ010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.563"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(44..780)
                     /locus_tag="ACHK49_005339"
                     /pseudo
     CDS             complement(44..780)
                     /locus_tag="ACHK49_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          247 a          195 c          207 g          274 t
ORIGIN      
        1 gttattttac ttttccctta tcgctgtcga ttctcattat cattcatggc aaaaaaacag
       61 caaaagaggc cctggtaatg actcctgtca gtagtacatg tgttttcgat aaatgaacca
      121 atggcttttc gtgaatttcc agggagcgta aaaagcaaat cgttttccgg gcaggtcgtt
      181 tgatgtgagt acgaagtgtc agattgttgc gctcaatgcg ccgggtaaat atcttgccga
      241 caagatgctt atcctgtggc atttctctgg tataactgct ccggttgtct ctggttatca
      301 tgcctgcgga gaatggcttc aggaattccg gcaactcacg gcatgtttca tcagtgcgag
      361 gaccaaaagt gtaagccagc acaccgtcag ctttggtctt atacgcgtac cagtgccact
      421 gctgacgagc tttgttttcg acaaaactcc attgttcatc aagttcacag ataagtgcca
      481 catcgacact ggcggctgga gatatggtta ctttacgtgg tgagcgtttt ttaaagtgag
      541 aatgacggtg ttgatatcca ccttcagcgt tctggagcta tcgcgaaccc cggctccgtt
      601 atgaaccatt tcaacaattt gctctttaat gcctggtttt cgggtttcgt agatgtaatt
      661 cagttgaaat acacgtttgc aggattagca ctggaaacgc tcatgatctg aggtgctgtg
      721 atcataacgg tacactttat aggaattaca gcggggagaa tatactgtaa ctgttgccat
      781 atggtctcca gataccaata gaatacaaca ttaatctatc gtcagaaggc atcaccgggg
      841 ccttctgaca taatctgtta atacgtgtac tattaccctg ttcagaaaat attgattcaa
      901 aaatgaaaac cagttaacag aaa
//
LOCUS       DAZGLJ010000551          922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000551 DAZGLJ010000000
VERSION     DAZGLJ010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.564"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>922)
                     /locus_tag="ACHK49_005340"
     CDS             complement(<1..>922)
                     /locus_tag="ACHK49_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO9265771.1"
                     /translation="NNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGNDVS
                     LGRSDAFSELPENTIVVPYGELTGSFEIIITTYPDLVVTFRELTAQEPEDRTLTLNQQ
                     IEVLGLKFAVKEKNEVWQYSLPGIIENNIISTKQHFFSSKLFKYVMLFFLFAIIFIAF
                     YIVNASNDPQLRHIDKILVNKNRNYEVLYGRDHVIYINTNILDEAVWVKQALEKNQPG
                     KPVRVINPDDESIRIFSWLADNFPDLQYFKLQLLDASNLRLTVSKQRNAITQQLIDNL
                     IKGLLQTMPYASNISIAVLDDNVLESQAIET"
BASE COUNT          299 a          169 c          138 g          316 t
ORIGIN      
        1 acgtttcaat agcctgactt tccaatacat tatcatctaa taccgcaata cttatattgc
       61 tggcatatgg catagtttgc agtaatcctt taataagatt atcgattagt tgctgtgtga
      121 tagcgttccg ttgcttactc actgtcaatc ttagattact ggcatctaat agttgaagtt
      181 taaaatattg taaatcaggg aaattatcag caagccatga aaaaattcgt atcgattcat
      241 catcaggatt tatcacccgt actggctttc ctggttgatt tttttccagt gcttgtttga
      301 cccagactgc ttcatccaaa atattggtat taatatagat aacatgatct ctaccatata
      361 aaacttcata attcctgttt ttgtttacaa gaattttatc gatatgtctc agctgcggat
      421 cattactggc attaacaata taaaaagcaa tgaaaatgat agcaaaaaga aaaaaaagca
      481 ttacatactt aaacagctta ctgctaaaga aatgttgttt tgtagaaata atgttatttt
      541 caataatgcc tggcaatgaa tactgccaaa cttcattttt ttcttttaca gcaaatttga
      601 gacctaaaac ttctatttgc tgattcaatg ttaacgttct atcttcaggt tcttgtgctg
      661 ttaattctct gaatgttact accagatcag ggtaggtagt aattattatc tcaaaactac
      721 ctgtaagctc gccataagga acgactattg tgttttctgg taactcagaa aaagcatcac
      781 ttcttcctaa cgaaacatca ttaccaatga taacaagtag acgcccatta agtatttcat
      841 actcgcatcc attcaatggt ccgctcaaca aacgaatggc ataagattcc aataagtctt
      901 gtgataagaa tttatcatta tt
//
LOCUS       DAZGLJ010000552          921 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000552 DAZGLJ010000000
VERSION     DAZGLJ010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.565"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(133..363)
                     /gene="ynfN"
                     /locus_tag="ACHK49_005341"
     CDS             complement(133..363)
                     /gene="ynfN"
                     /locus_tag="ACHK49_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HFO9265772.1"
                     /translation="MKKIIVFFNSEPAVVVPAMTGVNTIMREYPNGEKTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAIRLLS"
     gene            complement(368..433)
                     /gene="ynfR"
                     /locus_tag="ACHK49_005342"
     CDS             complement(368..433)
                     /gene="ynfR"
                     /locus_tag="ACHK49_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YnfR"
                     /protein_id="HFO9265773.1"
                     /translation="MKAPSGAFLLGIYSMDTHILR"
     gene            complement(436..624)
                     /gene="ynfQ"
                     /locus_tag="ACHK49_005343"
     CDS             complement(436..624)
                     /gene="ynfQ"
                     /locus_tag="ACHK49_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO9265774.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(635..847)
                     /gene="cspI"
                     /locus_tag="ACHK49_005344"
     CDS             complement(635..847)
                     /gene="cspI"
                     /locus_tag="ACHK49_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HFO9265775.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT          309 a          182 c          163 g          267 t
ORIGIN      
        1 agtaagtcga ttatatggtg caaacgtgta aaagataaga tgaaacatcg caataatcaa
       61 catacgatag tctaaatttt acacaaacag acaaagagaa ttttcctgaa ttatcaatac
      121 aatagcatca aatcaactca agagccttat tgctgcttcc agaatttctt ctgaagtaac
      181 atgtcgatcc gcggctacat aaatgacttt atgatctccg gtcagagatg gaaaccctgc
      241 ggccattaca gtaaggtgtg ttttttcgcc atttggatat tcacgcatga tggtgttaac
      301 tccagtcatc gctggcacta ccactgctgg ttcagagtta aaaaaaacta tgattttttt
      361 catgatgtta ccgtagtatg tgagtatcca tcgaatagat accaagcaaa aaagctcccg
      421 aaggagcctt cattttcact tttttaaatc caacgacaga cggctggcat ttaagtattg
      481 tgaaatatta tcaaatgtaa tcatcattga tttacaaaag atacattttg ccccgaaagg
      541 attcatgtca gaaacatcaa aagatgatgt tctatactgg gaaccatgac aacacgggca
      601 tctaaagtga atatggtttg taatattgtc tacctcaaag cgccactaca tgaacagcgg
      661 caggaccttt aggtccgttc tcaataccaa attcaacttc ctgattctca gttaatgttt
      721 tgaaatcgtt gctctgaatt gctgagaaat ggacaaacac atctttgctg ccatctttcg
      781 gcgtgatgaa accaaaacct ttttcagggt taaaccattt cactaaacca gtcattttgt
      841 tagacataat tattaccttt tgaagaaatt agcccttggg cagaatggtc cgaaaaaaaa
      901 tatcagagag aaaaaccaac a
//
LOCUS       DAZGLJ010000553          918 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000553 DAZGLJ010000000
VERSION     DAZGLJ010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.566"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>918
                     /locus_tag="ACHK49_005345"
     CDS             <1..>918
                     /locus_tag="ACHK49_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO9265776.1"
                     /translation="AGVRGGEQFDAINQSVARFASASGVEVDKVAEAFGKLTTDPTSG
                     LMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIATKGFDEQTRRLKENMGTLE
                     TWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDEADKKWQWYQSRSQRRGKTS
                     SFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARDRAERDASQLKYTGEAQKAY
                     ERLLTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYESTLKKPKSSGVKVSA
                     GERQEDQAHAALLALETELRTLEKHSGAN"
BASE COUNT          232 a          225 c          308 g          153 t
ORIGIN      
        1 atgccggtgt gcgtggtggt gaacagtttg atgccatcaa ccagagtgtc gcgcgttttg
       61 cgtctgcatc cggtgtggag gtggataaag tcgctgaagc cttcgggaag ctgaccactg
      121 acccgacgtc gggactgatg gcgatggcgc gccagttccg taacgtgacg gcagagcaga
      181 ttgcgtatgt tgcacagctg cagcgttccg gagacgaggc cggggcattg caggcggcga
      241 acgatatcgc cacgaaaggc tttgatgagc agacccgtcg cctgaaagaa aacatgggaa
      301 cactggagac ctgggcggat aaaacaggga aggcattcaa atcgatgtgg gatgccattc
      361 tggatatcgg tcgtccggaa tcctcagcgg atatgctcgc cagtgcgcag aaggcatttg
      421 atgaggcgga taaaaaatgg cagtggtacc agagtcggag ccagcgccgc ggtaaaacct
      481 cctctttccg ggccaacctt cagggcgcat ggaatgaccg ggaaaatgcc cgtctggggc
      541 tggcagcggc cacgctgcag tcggatatgg aaaaagccgg tgaactggcc gccagggacc
      601 gggccgaacg ggacgcatca cagctgaagt ataccggaga ggcgcagaag gcgtatgagc
      661 gtctgctgac gccgctggag aaatataccg cccgtcagga agaactgaat aaggccctga
      721 aagacgggaa aatcctgcag gcggattaca acacgctgat ggcggcggcg aaaaaggatt
      781 atgaatcgac gctgaaaaag ccgaagtcgt caggagtcaa agtgtcagcc ggtgagcgtc
      841 aggaagacca ggcgcatgct gccctgctgg cgcttgaaac cgagctccgg acgctggaaa
      901 aacacagcgg tgcgaatg
//
LOCUS       DAZGLJ010000554          914 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000554 DAZGLJ010000000
VERSION     DAZGLJ010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.567"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..221
                     /gene="etgA"
                     /locus_tag="ACHK49_005346"
     CDS             <1..221
                     /gene="etgA"
                     /locus_tag="ACHK49_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE muramidase EtgA"
                     /protein_id="HFO9265777.1"
                     /translation="EDDLLNDACINISVAGYILASNIKSRGNTWDAVGAYNAGYFNTP
                     NAVELRRQYAMKIYKTYTKLKNNEQIID"
     gene            complement(218..>914)
                     /gene="escU"
                     /locus_tag="ACHK49_005347"
     CDS             complement(218..>914)
                     /gene="escU"
                     /locus_tag="ACHK49_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFO9265778.1"
                     /translation="KPSAQKISVKNNLKNIFSVKSIFELLKSVFKLVIITLIFYFMGH
                     SYANEFANFTRLNAYQTLVVVAFFVFLLWKGVLFGYLLFSVFDFWFQKHEGLKKMKMS
                     KDEVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKKSTVIVKNPTHIAICLYYKL
                     GETPLPLVIETGKDAKALQIIKLAELYDIPVIEDITLARSLYKNIHKGQYITEDFFEP
                     VAQLIRIAIDLDY"
BASE COUNT          303 a          167 c          151 g          293 t
ORIGIN      
        1 ctgaagatga tttattgaat gatgcctgta taaacatatc tgttgctggt tatatcttag
       61 cctctaacat aaaatccagg gggaatacat gggatgcagt tggtgcgtat aatgcaggct
      121 actttaacac accaaatgca gtagaactca gaagacaata tgcaatgaaa atatataaaa
      181 catacactaa attaaaaaac aatgaacaaa ttatcgatta ataatcaaga tctattgcta
      241 tacgaatcaa ttgtgctaca ggttcaaaaa agtcttctgt tatgtattgt cctttatgta
      301 tgttcttata aagacttctt gccaaagtaa tatcttcaat cactggaata tcatagagtt
      361 cagccagttt aatgatctgt agagctttgg catcttttcc tgtttcaata actaaaggca
      421 atggagtctc cccgagttta tagtataggc aaatcgcaat gtgagtcggg tttttgacga
      481 tcacggttga ttttttgatg ttattagcca gacttccact ttgtatctca gaatgaaggc
      541 gacggcgctc ccctttaatt tcagggttac catcagtatc cttggcttct cgtttcacct
      601 catctttact cattttcatt ttcttcagtc cctcatgctt ctggaaccag aaatcaaata
      661 ctgaaaagag tagatatccg aataacacgc ctttccataa aagaaaaaca aaaaaggcaa
      721 caacgacaag agtttgatat gcgttcagtc ttgtgaaatt tgcaaactca tttgcatatg
      781 aatgccccat aaagtaaaaa atgagagtaa ttatcactaa cttaaatact gacttaagca
      841 gctcaaaaat actctttaca gaaaaaatat ttttcaggtt attttttaca ctaatcttct
      901 gagccgatgg tttt
//
LOCUS       DAZGLJ010000555          900 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000555 DAZGLJ010000000
VERSION     DAZGLJ010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.568"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..808
                     /locus_tag="ACHK49_005348"
     CDS             <1..808
                     /locus_tag="ACHK49_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFO9265779.1"
                     /translation="RMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGK
                     RFINGAVIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQ
                     DLSPSGPLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEY
                     KKLFALPDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNAL
                     LNILESQFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
BASE COUNT          248 a          181 c          217 g          254 t
ORIGIN      
        1 cagaatgacg ttatccctca tggtgcaacc cggtcttttt gaccgatata tggagcgaaa
       61 aggttctgtt gccagggaca gtggttttct tgcccgttgt cttatcagta aacctgcaac
      121 cacgcagggg aaacgcttta ttaacggagc tgtgattccg ggaggatcat taacggcatt
      181 tcatgaacga ttaatggaac tggccagagg cagtattgaa aaatccagtg aggatgaacg
      241 gtattgtctg catttttcgc ctgaagcgca aaaaatattt attgagcact ataatgttct
      301 ggagcaggat ttatcccctt ccggaccctt aagcccgttt cggggacatg tttcaaaaaa
      361 aacagaaaac attgcaagaa ttgcagcgtt atttcagtat ttcagctatg gtgaagggaa
      421 aatctctgcc gatatcatga cgtctgctgt cgtaataagt tcgtggtaca cagatgaata
      481 taaaaaatta tttgcgttac ctgatgaatc tgaactgcag cagaaggacg ccgaggagct
      541 gttcgactgg ctcattgagg agtgccgcgg agaatgcccg ccccgtgtca ggaaaaatta
      601 tattctccag tgtggcccgg gccggttcag aaacaggaaa aaactgaacg cattgctgaa
      661 cattcttgaa tctcagttca ggctttctgt cgtgccggaa ggaaaaacca tgtatgttct
      721 gcttccccag atagccagtt taaagttgtc tgatgtctct ggtattttta cctcgggtta
      781 tcactacaat aagctgagag cgaagtaacc ggtgagcagt ttttctccat tatgcttcag
      841 acttagatga ttgattatta tggggatttc atcaccgata ttcagatgta tgaatatcgg
//
LOCUS       DAZGLJ010000556          900 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000556 DAZGLJ010000000
VERSION     DAZGLJ010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.569"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>900)
                     /locus_tag="ACHK49_005349"
     CDS             complement(<1..>900)
                     /locus_tag="ACHK49_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO9265780.1"
                     /translation="TRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNT
                     VGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSIT
                     DVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGA
                     GIENTPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITS
                     GGNPPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCQIKGKLSANQIEGDIV
                     KTVGKAFPRDSRAPERWPSGTITV"
BASE COUNT          190 a          252 c          208 g          250 t
ORIGIN      
        1 gacggtaatg gtccctgatg gccaccgctc cggtgcccgg gagtcccgcg gaaaagcctt
       61 acccactgtt ttgactatat cgccttcaat ctggttggct gacagtttcc ccttaatctg
      121 acagttctca ttaattgtga cattgttgag cgtcccggcg ttcgcattca cactgccact
      181 gatatctgca tttttagcgg tcagctttcc gtccggtgtc agggaaaatg ccggaggatt
      241 accgccgctg gtaatggtgg gagccgtcag gcgtttcagg aacacgtcgt tcatgaatat
      301 ctgatcgccc tgaccaacaa acatcggctt tgtgttgcca ttcgcaggat taatcatcgc
      361 aatcctgtct gccgccagca gcacctgact ctgcattcct gctggcgtat tctcaatacc
      421 ggcaccgata cccgcaatat aaaggcgtcc gtccttcatc tgttgcagct tcacagccca
      481 catgctgttc agattattat ttgtatcaac ctgaaccttc tgtatctgct ggatcgctgc
      541 actctggtct tccagtttct tattgacggt ctgcgtgatt tcattactga catccgtgat
      601 ggacgttctg atttccgcca ggtcaggcgc aagctgaccg ttatcaatct gagtccacaa
      661 ctcctgagcc agatgggttt tccctatctc tcctttgaaa aaatccagat agccggatgc
      721 atcatcactc ggctggccaa cagcctccac aaatgccgat ttgccaacgg tgttcacact
      781 gcggatataa aagtaataat catggcccgg tttgatattg atactggcgg ctatccagta
      841 cagccccgtg ccaaggtagc gggctgtggt ttcaacctgc ctgatatcgg taatccgcgt
//
LOCUS       DAZGLJ010000557          896 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000557 DAZGLJ010000000
VERSION     DAZGLJ010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.570"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            91..336
                     /locus_tag="ACHK49_005350"
     CDS             91..336
                     /locus_tag="ACHK49_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265781.1"
                     /translation="MDIRHSLNEWWTLWQASRAAIELDIDWPESNDDFWKDGEEGAYA
                     MGYEDGRDKTVIAVMKAIRAAGIKEKNFDEANNIPPN"
     gene            308..718
                     /locus_tag="ACHK49_005351"
     CDS             308..718
                     /locus_tag="ACHK49_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO9265782.1"
                     /translation="MKQTIFLRTKQQQQAAINAILATPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCDKWLKPEQWKVLLISGHAVATKQEADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            715..891
                     /locus_tag="ACHK49_005352"
     CDS             715..891
                     /locus_tag="ACHK49_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO9265783.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
BASE COUNT          292 a          189 c          226 g          189 t
ORIGIN      
        1 tttgaggaat acgttgccaa aaaattgaga ttaccattcg agatgataac cgaggcaaga
       61 aatggtgata ggtacttcgc attttcaagc atggatattc gtcactcctt aaatgagtgg
      121 tggactttat ggcaggcatc gcgagcagct attgaactgg atatcgactg gccagaatcg
      181 aatgacgact tttggaaaga tggtgaagaa ggtgcttatg cgatgggtta tgaggatggg
      241 cgtgacaaaa cggtaattgc agtaatgaaa gctatcagag ccgctggaat taaagagaag
      301 aatttcgatg aagcaaacaa tattcctccg aactaagcaa caacagcaag ccgcaatcaa
      361 cgccatcctc gcaacaccac tcgataaaga caagccagtc accatccgca ttactgacta
      421 caagcgcaac cttgaccaga acgcaaaatt tcacgcgatg ctggcggata tcgcacgtca
      481 ggttcaatgg tgcgataagt ggttaaaacc agaacaatgg aaggttttgt tgattagcgg
      541 tcatgcagtg gcaacaaagc aggaagctga tgttttgccc ggccttgaag gcgaatacgt
      601 caacattcgc gaaagtagcg cgcagatgag tgtgaagcgt atggcaagtc tgattgagta
      661 cacgacagca tgggctattg gtcagggcgt cagatttacc gacaggaggt acgaatgaga
      721 cgacagcgac gaagtatcac cgacataatc tgtgaaaact gcaaatacct tccaacgaaa
      781 cgctccagaa ataaacgcaa gccaatccca aaagaatctg acgtaaaaac cttcaactac
      841 acggctcacc tgtgggatat ccggtggcta agacatcgtg cgaggaaatg acaatg
//
LOCUS       DAZGLJ010000558          895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000558 DAZGLJ010000000
VERSION     DAZGLJ010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.571"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..672
                     /locus_tag="ACHK49_005353"
     CDS             <1..672
                     /locus_tag="ACHK49_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9265784.1"
                     /translation="QAVRLMGCINVIVGVPGVGKTATARNYCQEQPNTWMITLSPAHS
                     SVTECLLELADALGIDYTRANKGALSRAIRRRLMGTRGLVIVDEADHLGIDGLEQLRA
                     IQDATGIGMVLIGNPRGLFKGGRRAFDDLSRLFSRLARTKQLRKAKKADVLAIARAWG
                     ISGEAELAVMQAIAEKPGALRVLTHTLNQAWLTASGEGAALTEKHINAAFKEVYTNPE
                     LLSQV"
     gene            675..>895
                     /locus_tag="ACHK49_005354"
     CDS             675..>895
                     /locus_tag="ACHK49_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265785.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATDVYSGICRSVGAKQVS"
BASE COUNT          204 a          213 c          267 g          211 t
ORIGIN      
        1 caggcggttc gtctgatggg ctgtattaac gttattgtgg gcgtaccggg tgtgggtaaa
       61 acggccactg cccgtaatta ctgccaggag caaccaaaca cctggatgat caccctgtca
      121 cccgcgcact ccagcgtcac ggagtgtctg ctggagctgg ccgatgcgct ggggattgat
      181 tacacccgcg cgaacaaagg ggcattatcc cgcgccatcc gccgtcgcct gatgggaacg
      241 cgtggactgg tgattgtgga tgaggcggat catctgggta ttgacggtct ggagcaactc
      301 cgggcaattc aggacgccac ggggatcggg atggtgctta ttggtaaccc gcgcggattg
      361 tttaaaggtg gacgccgcgc ctttgatgat ttatcgcgcc tgttcagccg tcttgcccgt
      421 acaaaacaac ttcgcaaggc caaaaaggcg gatgtgctgg ccattgccag ggcgtggggg
      481 atcagtggtg aggccgagct ggccgtcatg caggctatcg ctgaaaagcc gggagcgtta
      541 cgcgttctga cacatacgct taaccaggcg tggctcaccg ccagcggtga aggcgcggcg
      601 ctgacagaaa aacatattaa tgcggccttt aaagaggttt ataccaaccc tgaattactc
      661 tcacaggtgt gattatggcg gtatttaata ttcctgatat ttacggacgc ttttacctgg
      721 ttaatttcga taacgtgaag gtgatttcac tggccgaaaa taaagaatgt ggcgatttac
      781 tttttgaatt taatgaccgc acacgaatgg tgatatctgc cggacttgat cgcgaaggtg
      841 cgacagacgt ttacagcgga atatgccgtt ctgttggtgc gaaacaagtc agcta
//
LOCUS       DAZGLJ010000559          894 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000559 DAZGLJ010000000
VERSION     DAZGLJ010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.572"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            8..>894
                     /locus_tag="ACHK49_005355"
     CDS             8..>894
                     /locus_tag="ACHK49_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9265786.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRL"
BASE COUNT          278 a          194 c          154 g          268 t
ORIGIN      
        1 gtagtgtatg gccaatctct acgacttgaa aaagttcgac ttaaaccttc ttgtcatatt
       61 tgagtgtatt taccagcatt taagcattag caaagctgct gaatcactat atatcacccc
      121 atcagccgtg agccagtcgt tacaacgctt acgtgcgcaa tttaatgacc ctttattcat
      181 tcgctcagga aaaggtatcg cgccgacgac aacagggttg aatctgcatc accatcttga
      241 gaaaaacctc aaaggccttg aacaaacaat taatatagtc aataagtccg aactaaagaa
      301 aaactttatt atttacggcc ctcagctcat ttcttgctct aataatagta tgctgatccg
      361 ctgcctacgg caagatgcct ctgtcgagat tgagtgccat gatatcctca tgtcagcaga
      421 gaatgctgaa gaactgttgg ttcaccgcaa agccgatctg gtgatcaccc aaatgccagt
      481 gatcagccgt tccgtcattt gcatgccatt gcatacaatt cgcaatactc taatttgtag
      541 taataagcat ccgcgaatta ctgacaatag cacttatgaa caaattatgg cagaagagtt
      601 cacccaatta atatccaaat cagcaggagt tgatgatatt caaatggaaa tagacgagaa
      661 gtttatgaat cgcaaaattt catttcgtgg ttcctctttg ctgacaatca ttaatagcat
      721 tgcagttaca gatttactgg gaatagtccc ctatgagctt tataattcct accgtgactt
      781 tcttaattta aaagagataa aactagaaca tccgttacct tcaataaagc tttacatttc
      841 ctataataaa tcttctttaa ataatttggt gttctcccga tttattgacc gtct
//
LOCUS       DAZGLJ010000560          888 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000560 DAZGLJ010000000
VERSION     DAZGLJ010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.573"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            161..>888
                     /gene="cif"
                     /locus_tag="ACHK49_005356"
     CDS             161..>888
                     /gene="cif"
                     /locus_tag="ACHK49_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000652081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector Cif"
                     /protein_id="HFO9265787.1"
                     /translation="MKDITLPPPTSASCLTGAISVNTEAVLSPMQHTSALHVRDFASL
                     CSQNLKANVLLNSDDHEVPIHQKNPAAIMQNIDSNIKQMATDWGMSIEEVEVIIGREK
                     GIVEPSCGVTANAIMKLFLDKDGFSYCFENEQTLSLEQLQERLSCMPECKSFVLRVND
                     GALGHAYIVDIPKGENSCRPAFLYQSDLGEGVTRKLRFEDWMTHKALTPILLDDICNY
                     FSCMSQNKTDLEQIATLFDIDGNV"
BASE COUNT          264 a          183 c          199 g          242 t
ORIGIN      
        1 caatccaggc cgcgtcacag aaatggacaa tgccgctgag agactggcga atggcataca
       61 ggcaccagaa aaatttcaga catgacataa tgcccatgcc agaagttctt tttgcacctc
      121 gcctgtattt atagataaat atagagtgaa tgaatgagat atgaaagaca ttacccttcc
      181 ccccccgacg tccgcgtcct gtctgacagg ggccatatct gtaaatactg aagctgtatt
      241 atctcccatg caacacactt cagccttaca tgtaagagat tttgcttccc tgtgctcaca
      301 gaacctcaaa gctaatgtat tgctaaatag tgatgaccac gaagtaccta tacatcagaa
      361 aaatcctgct gcaataatgc aaaatatcga ctctaacatc aaacagatgg caacagactg
      421 ggggatgtcg attgaggagg ttgaggttat tatagggcga gagaaaggca ttgtggaacc
      481 ctcctgcgga gttaccgcta atgctattat gaaactattt ctggacaagg atggcttcag
      541 ttactgcttt gaaaatgaac agacactatc gctcgagcag cttcaggagc gcctgtcctg
      601 tatgcctgaa tgtaagagct ttgtattacg tgttaatgat ggtgcgcttg gtcatgctta
      661 cattgtcgat attcccaaag gagaaaactc ttgtcgtcct gcattcttgt atcagtcaga
      721 tttaggagag ggcgtcacca gaaagttaag atttgaggac tggatgacgc ataaagcatt
      781 gactccgatt ttgctggatg atatttgtaa ttacttctcc tgcatgtctc aaaataagac
      841 agatttggag cagattgcaa cgttatttga tattgatgga aatgttaa
//
LOCUS       DAZGLJ010000561          881 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000561 DAZGLJ010000000
VERSION     DAZGLJ010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.574"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            158..373
                     /locus_tag="ACHK49_005357"
     CDS             158..373
                     /locus_tag="ACHK49_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO9265788.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKTARGD"
     gene            378..722
                     /locus_tag="ACHK49_005358"
     CDS             378..722
                     /locus_tag="ACHK49_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO9265789.1"
                     /translation="MKKKYELVVKEINNYPDKIAVTVALEIGGHPSLLLPHVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            773..>881
                     /locus_tag="ACHK49_005359"
     CDS             773..>881
                     /locus_tag="ACHK49_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9265790.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPEILDQFLDEKEG"
BASE COUNT          238 a          179 c          250 g          214 t
ORIGIN      
        1 catcataaaa agtgagccag tcaaatggaa ggtatcgtta aactcaccgg tagtgtcagt
       61 gggtcgtctg agacgcctgc atgagttatc agagccatca gtagttaact ggtggctttt
      121 ttattgttgt cagcttccgg ataacgggag acggggtatg taccagatgg aaaaaatcac
      181 aacaggtgtg tcatacacca cgtcagcggt agggacggga tactggttac tgcaactgct
      241 ggacaaagtc tctccgtccc agtgggtggc aataggtgta ctggggagtc tgctgtttgg
      301 cctgctgacg tatctgacta acctgtattt caaaatcagg gaggaccgcc gtaagacggc
      361 gcggggagac taaagcgatg aagaaaaaat acgaactggt tgttaaagag ataaataatt
      421 acccggataa gattgctgtt actgtggcac ttgaaattgg cgggcatccg tcgttgttgt
      481 tgccacatgt ggcgattagt cttgaccgta ctgaaggtgc cacgctggag ttttacgaag
      541 ctgaggcgaa aaagcaggcg aagcaatttt tcatggatgt tgctgccggg ttatgtgaag
      601 gggatggtcc gttaccggaa aagcgtcccg taattttaga ggcgcaggat gtgttgataa
      661 cctacagagg aaaactaccg ggaataatta cgggttctct gaagactcca ccgctggcct
      721 gaagacttaa catatccagg gatttgaaat cgataaaccc tgataaatat ccatgaacgc
      781 aaaaatcaga tacggcctgt cggctgccgt tctggcgctg attgccgcag gtgcgcctgc
      841 gcctgaaatc ctcgaccagt ttctggatga aaaggaaggt a
//
LOCUS       DAZGLJ010000562          876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000562 DAZGLJ010000000
VERSION     DAZGLJ010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.575"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..608)
                     /locus_tag="ACHK49_005360"
     CDS             complement(<1..608)
                     /locus_tag="ACHK49_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265791.1"
                     /translation="MDMIPSYESMYLTLRGIPTDSIGGEDIYFIHDPQGGWYPNRGNH
                     LYAIDALCVNIHDALANSIFDGLENYHKFLYMAPEFLSTAGLNSESVVSKETFVLFID
                     KFKGHTDVNKGLYLFDCCKIVSSIQECSKEVLQLQGEFYYTLNFEPLFFPNIEEEDGI
                     RYVTSPVVTKLFALLGFIYIRMYSLLDYITKLAIEIENLKTQ"
BASE COUNT          280 a          169 c          143 g          284 t
ORIGIN      
        1 aactgtgttt ttaaattttc aatttctata gctagttttg ttatgtagtc gagcaaactg
       61 tacatgcgga tataaataaa tccaagtaat gcaaagagct tagtcacgac tggagatgta
      121 acatatctta taccgtcctc ctcttcaatg ttagggaaaa acaagggttc aaaattcaac
      181 gtgtaataaa actccccttg tagttgcaac acttccttgc tgcattcctg tatgctactt
      241 acaattttac agcaatcaaa aaggtaaagg cctttattta cgtcagtatg ccctttgaat
      301 ttatcaataa ataaaacaaa tgtttctttt gaaactacgg attcagagtt caaacctgct
      361 gttgataaaa actcaggggc catataaagg aacttgtggt agttttctaa tccatcaaaa
      421 atgctattag ctagagcatc atgaatatta acgcataatg catcgatcgc ataaagatga
      481 ttccctctgt tcgggtacca tccaccttga ggatcatgta taaagtaaat gtcctcacct
      541 ccaattgagt cagttggtat ccctcttaaa gtcaaataca tgctttcata tgaaggtatc
      601 atatccatgt ttttctttaa ttcctcttat gaatcgaaaa agtgaggttt cgtatatagg
      661 ttaatttaag caagataatc atactctgaa agtaataatt tttcatatcc gggtttatca
      721 aacgcaggga attttatggg cttttcttta ctcaacctaa cgcccgcttc tggcacagag
      781 cggactgtca gattagattc agacctgtac tcattagctg atacaaatca gccacatata
      841 cctgcattcc aaataatttc agacagatat tatcgt
//
LOCUS       DAZGLJ010000563          872 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000563 DAZGLJ010000000
VERSION     DAZGLJ010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.576"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..375
                     /locus_tag="ACHK49_005361"
     CDS             <1..375
                     /locus_tag="ACHK49_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO9265792.1"
                     /translation="GATRVDGKPVIPGMKLSKGKCDQVNAIERDKALAWVEKNIKVPL
                     TEPQKAGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSN
                     NCYGQVSRRDQESALACWGIDR"
     gene            674..>872
                     /locus_tag="ACHK49_005362"
     CDS             674..>872
                     /locus_tag="ACHK49_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFO9265793.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDA"
BASE COUNT          232 a          182 c          250 g          208 t
ORIGIN      
        1 ggagccaccc gggtggatgg taagcctgtt attcctggca tgaagctgtc gaaggggaaa
       61 tgcgaccagg ttaacgccat tgagcgtgat aaggcgctgg catgggtgga gaaaaacatc
      121 aaagtgccac tgactgaacc ccagaaagcg ggtattgcgt cattctgtcc ttacaacatt
      181 ggcccaggta agtgtttccc gtcgacgttt tataaacgaa ttaatgcagg agatcgaaaa
      241 ggtgcctgcg aagcgattcg ctggtggatt aaggacggtg gcagagactg ccgtattcgc
      301 tcaaataact gttatggtca ggtctcacgg cgtgaccagg agagcgcgct ggcgtgctgg
      361 ggaattgaca gataagcaga atattttgct gaaaaatgcg gtttgctcac acggacggat
      421 aacacaaaat cctgcgaact gacaaaaact aagtgaataa aagtaaaaac cccatttgtt
      481 ggctgcaagc ggggttttgt gtttcctgac tccggaaaag tcaaaggaga aagtgtgttt
      541 gattttagca aactgattcg ggagattcga gtgatggctg aaaaattatc aacctggaag
      601 tacatcctta tctggctggt gtttgtgatt atggcctccg gttatttcat cggtcagata
      661 cgctggtggt gaaatgaacc gcgtactgtg cgtggtcatc attgccctgc tggtggcctg
      721 tggtgcgctt agtctggggc tgaatcatta ccgcgataac gccatcacct acaaagcgca
      781 gcgcgataaa aaagccagag agctggagct ggcaaacgca actattactg atatgcaggt
      841 gcgccagcgc gatgttgctg cgctcgatgc aa
//
LOCUS       DAZGLJ010000564          869 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000564 DAZGLJ010000000
VERSION     DAZGLJ010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.577"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            287..562
                     /locus_tag="ACHK49_005363"
     CDS             287..562
                     /locus_tag="ACHK49_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265794.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFPDDIIP"
     gene            787..>869
                     /locus_tag="ACHK49_005364"
     CDS             787..>869
                     /locus_tag="ACHK49_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265795.1"
                     /translation="MIKKISINFLFLMLMIDVVFATLFNIPV"
BASE COUNT          252 a          141 c          189 g          287 t
ORIGIN      
        1 tccttgttat ataaaagatg atgcctttcc agtgagtttt cgtctgaata atacgaatgt
       61 ttgttttcgt catgaagcgt tccggctccc ggggctatac atcgccagaa aagcaatgca
      121 tattgtcggg attatccaga gaatcaggca gaaagatccg acaatgggct gtgattatgc
      181 catctggctc agaatgatat caaggtgtta tggggataac ttgctgatac aatagcagta
      241 tgacagattc attaatgcgg aaactgctca ctgaaacaga gtgttcatga ctgagcactg
      301 gtcgggtcat gcagatgata atttcccctt ttcttgggga agcgacagta ttttcatgca
      361 tgcctgtctt cagtcatacg gggagagcat ctgtgataca gtccatacat ttgttgcgga
      421 cgtcgctcct gagtcactga ctggtctgca aacagtttat ctgtacacgg cagggcttct
      481 gtgtgatatc gtttttcata tcgagggggg gcctccagag atgaatggaa taataatatt
      541 tcctgatgat ataatacctt agagaatttc tgtgtatgct attgtgaaca tattttttat
      601 taaaaagatg ttagtggtgt tgtaaaataa aagaatcagt aactataaac gcctttattg
      661 acataaagtt ttgatgatga agttgaagtt tgacctggta taaatcagta ttcactttac
      721 caggttttat aaaaatatat cacttttaga tacggacaat tatagactct aattaatggg
      781 ggtaagatga ttaaaaaaat cagtattaat tttcttttct tgatgttaat gatagatgtt
      841 gtttttgcca cattgttcaa tatccctgt
//
LOCUS       DAZGLJ010000565          869 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000565 DAZGLJ010000000
VERSION     DAZGLJ010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.578"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..761
                     /gene="ydeO"
                     /locus_tag="ACHK49_005365"
     CDS             <1..761
                     /gene="ydeO"
                     /locus_tag="ACHK49_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO9265796.1"
                     /translation="SLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYE
                     RHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNH
                     HNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLY
                     ISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAF
                     RKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
BASE COUNT          260 a          172 c          174 g          263 t
ORIGIN      
        1 tgtcgctcgt ttgttctgtt atatttattc atcatgcctt caacgctaac attttagata
       61 aagattacgc cttctctgac ggcgagatcc tgatggtaga taacgctgtt cgtacgcatt
      121 ttgaacctta tgagcggcat tttaaagaga tcggatttac tgaaaatacc attaaaaaat
      181 atctacaatg cactaacatc cagacagtga cggtgcctgt tcctgcgaag tttttacgtg
      241 cttcaaatgt accgactgga ttgcttaatg aaatgattgc ttatctcaac tcggaagaac
      301 gcaatcatca taatttttca gaacttttgc ttttttcttg cctgtctatt tttgccgcat
      361 gcaaaggttt cattacacta ttaactaacg gtgtgctatc cgtttccgga aaagtgagaa
      421 atattgtcaa catgaagctg gcgcacccat ggaagctgaa agatatttgt gactgcctgt
      481 acatcagtga aagcctgttg aagaaaaaac taaagcaaga gcaaacgaca ttctcacaga
      541 ttctattaga tgccagaatg cagcacgcaa aaaatttgat acgcgtagaa ggttcagtca
      601 ataaaattgc cgaacaatgt ggttatgcca gtacatctta ttttatttat gcgttccgca
      661 aacatttcgg caacagtccg aagagagttt ctaaggagta ccgttgtcaa agtcacacgg
      721 gtatgaatac gggcaacacg atgagtgctt tagctatttg attatttgct aacgagtagt
      781 caaccacgca cgctacgtaa gaattatatg gggcagccat tccctgcccc gcgttgtttt
      841 taggcgatat atttattgaa ataaataag
//
LOCUS       DAZGLJ010000566          864 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000566 DAZGLJ010000000
VERSION     DAZGLJ010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.579"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(146..220)
                     /locus_tag="ACHK49_005366"
                     /pseudo
     CDS             complement(146..220)
                     /locus_tag="ACHK49_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(217..642)
                     /locus_tag="ACHK49_005367"
     CDS             complement(217..642)
                     /locus_tag="ACHK49_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9265797.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
     gene            729..864
                     /locus_tag="ACHK49_005368"
                     /pseudo
     CDS             729..864
                     /locus_tag="ACHK49_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          216 a          200 c          215 g          233 t
ORIGIN      
        1 gggcgcctga ttaaaatctg aggaaacata taataatttt catcaaaata gtatttatgt
       61 actaactaag ctggatttga acctctattt ctgggtattt gttatcactt aatccattac
      121 tgctcctcct tcagcatatg tcgtatccgt tgaaggattt acgcatatcg gtaacgtcgg
      181 caacgagcca gatgcgggta cctgacggga gtgagatcat cgtcccctcc cggtcagttc
      241 acggatcaac agcgtgagca gctctggcga tggattttcc agcgtcatgt taccgtgacg
      301 gaactccacc ttgcaggagc cggcactgac actggtctga gtggaagtgg ataaagacgg
      361 cgcaatggcc gccaccggtt ctttctgctc atccggcgtt atttctacag gtaataattc
      421 aacgccagtg tcagaagagg tcgttacctg aagacgccgt gatatacgcc cttcgttctg
      481 ccagagcctg agccatttga acaggaggtt atcattgata tcgtgttccc gggcaatacg
      541 ggcaacagag gctcctggtt gtgatgccag ttccaccata cgaagtttga attcattcga
      601 atagttttta cgaggttctt ttcgccagtc ctgtaattcc atacttagat gtccgtctat
      661 atcagatggg cgtctaagtt accaattttc gtctgatggc tacatacggc ggtcagttta
      721 cgcttactga caacgcgatg gcggagagca tcaatggtct ttacaaagcg gaggtaatac
      781 accgtaagag ctggaaaaac cgtgcagaag tggaactggc cacactcacg tgggtggact
      841 ggtataacaa tcgacgattg ctgg
//
LOCUS       DAZGLJ010000567          858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.580, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000567 DAZGLJ010000000
VERSION     DAZGLJ010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.580"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>858
                     /locus_tag="ACHK49_005369"
     CDS             <1..>858
                     /locus_tag="ACHK49_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO9265798.1"
                     /translation="EVNYNEISDADKNIIKKILTEISKMPEDSIFTKINTRIPRDTMP
                     ILRRYHLWPDGWNIRGLNGFMLSHKGSEVIDAVIAGQNQAYRELRRIRDNIHSEIYFK
                     QTDELSSLPDTDKIGGILVKKYLSGSLFSKFRQDTIIPEALSTLQISGPDLIQRKMLQ
                     FFRSRGVLGEEFINERKLSDKAYIGVYKTTGTGKYDWLNPESIGVNDVTPADESTWCI
                     GKGRCVDDFLFKDVSKLKTENLPELFLTKIDTDTFFSQWSTKTKKDLQKKIQDLTVRY
                     NELIDSSTI"
BASE COUNT          311 a          146 c          171 g          230 t
ORIGIN      
        1 atgaagttaa ctataatgaa atcagtgatg cagataaaaa cattatcaag aagatattaa
       61 cagaaatatc taaaatgcca gaagatagca tttttactaa gatcaataca aggattcctc
      121 gagacacaat gcccatcctt cgtcgttatc acctatggcc tgatggatgg aatattcgtg
      181 ggctcaatgg attcatgcta tcacataaag gtagtgaagt gattgatgct gttatcgcag
      241 gccagaatca ggcttacagg gaactaagaa gaataagaga taatattcat agtgaaatat
      301 acttcaaaca aactgatgaa ttgtcctcac ttccagatac agacaaaatt ggagggattc
      361 tggtaaaaaa atacctttca ggaagtcttt tttcaaaatt cagacaagat actattatcc
      421 cggaggcatt gagcaccctc cagatatcag gtcctgactt gattcaaaga aagatgttgc
      481 aatttttcag gagtagaggg gtgttaggtg aagagttcat taatgaaaga aaactgagtg
      541 ataaagctta tattggtgtc tacaaaacaa ctggcacagg gaaatatgac tggttaaacc
      601 ctgaatcgat cggcgttaat gatgtcacgc ccgcagatga aagtacctgg tgtataggaa
      661 aaggccggtg tgttgatgac ttcctgttca aagatgtttc aaaactaaaa acagaaaatc
      721 ttccagaatt attcttaaca aaaatagata ctgatacgtt tttctctcag tggtcaacca
      781 aaaccaagaa agatctgcaa aaaaaaatac aagaccttac tgtacgttat aatgagctaa
      841 ttgactcgtc gactatcg
//
LOCUS       DAZGLJ010000568          858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000568 DAZGLJ010000000
VERSION     DAZGLJ010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.581"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..377)
                     /locus_tag="ACHK49_005370"
                     /pseudo
     CDS             complement(1..377)
                     /locus_tag="ACHK49_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          249 a          118 c          169 g          322 t
ORIGIN      
        1 ttgttttttt catatctaat gttattaatc tgattaagta ttgattgtgt ctgttgcgta
       61 agttgtgtcg aacttatcgt ttcaatgtta gtaataagac ttacaagttg agccacaact
      121 tttgagtagt ctgcatttgt atatgtagtg ttcgtgtggc acgcattaat ttgttgaagt
      181 tggtgttgcg ctaaatcata ttgatcagag atatatcctt ctccagagaa tgtggaatta
      241 atgggttatt gttcttccca attatcgcaa ggccacttcg gtgtgtctgg cgttttagca
      301 ttatcggtat ttgttgtgca gttataaata tcgccgtgat atgtatttat tatacctggg
      361 gtaaagtcaa ttgacataaa attccttatt catcatttgt tttatgctaa gatgtttaaa
      421 ttggataatt agaacaggaa tggattttct tctacacaca ataaatataa tgcgttatat
      481 cttcctgata taattacctt taaattccct ttcatttcca cccggtaata tttgtgtata
      541 aaaaaaggca gtgatagtat cgtaaaggga aacgttaaca actatcaaaa agcgctcatt
      601 ttaaaaagtt attttgttct ccgatagtgt gtgaagtaat atttttgtga tgttttagat
      661 gtgtaataat gaaccaggtt aaagtgtatt taaaaatgtg ttgtattagc gactgaaatc
      721 tgccttttaa aaaggaggag ggtactgaga caaagagtca ttgcttctcc gataaaatgt
      781 cagagaagca tgattacgtg gttattgtct gttgatgact tgttgaccga gccgtcatca
      841 ttctcaatgc attagtta
//
LOCUS       DAZGLJ010000569          856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000569 DAZGLJ010000000
VERSION     DAZGLJ010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.582"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..677
                     /gene="rfaY"
                     /locus_tag="ACHK49_005371"
     CDS             <1..677
                     /gene="rfaY"
                     /locus_tag="ACHK49_005371"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFO9265799.1"
                     /translation="VFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDDTKVILIDTARG
                     PLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSINDYFLLAERKTLN
                     FAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGDPHKGNFIVSEK
                     GLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKKIKKVIRDVKVK
                     LGLKSK"
     gene            702..>856
                     /locus_tag="ACHK49_005372"
     CDS             702..>856
                     /locus_tag="ACHK49_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO9265800.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILE"
BASE COUNT          312 a          117 c          157 g          270 t
ORIGIN      
        1 aagttttcat aaaagataat gatccctttt atgagcaagt tctaaatgac tttttaactt
       61 gcagggttaa aacattaaaa gtttttcgtt cgattgatga tactaaagtc attcttattg
      121 acactgctcg tggacctctt gtacttaaag tttatgcgcc aaagcataaa atgaccgaac
      181 gtttcttaaa atcttgcata aaaaaagatt attatgaaaa tttaatttat caaaccgatc
      241 gggtacgtgg tgaaggcatc caatcaatta atgactattt tttgctagct gaacgtaaaa
      301 cattaaattt cgctcattat tatattatgc tcattgagta tattgaaggt gtcgggttaa
      361 atgagtattt ggaaatttct gaagatctga aggatcaatt atcagaatcg ataaaagagt
      421 tgcatcagca tggaatggtg tctggcgatc ctcataaagg aaattttata gtatccgaaa
      481 aaggtttaag actaattgac ctttcaggta aaaaaacaac cgctgtttta aaagcaaaag
      541 atcgcattga tcttgaacgg cactataata taaaaaatga attgaaagac tttggctata
      601 catatcttat attcaaaaag aaaattaaaa aagtaatcag ggatgtaaaa gtcaaactag
      661 gattgaagag caaataatga ttatgatgaa gggtaattaa attggatttt aaacatctta
      721 ctcaatttaa agatataatt gaactggaca agcgccccgt taaacttgat gaacgggaaa
      781 cgtttaatgt ctcatggggt attgatgaga actaccaggt tggggctgcg atttcaattg
      841 cttcaattct tgaaaa
//
LOCUS       DAZGLJ010000570          856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000570 DAZGLJ010000000
VERSION     DAZGLJ010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.583"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(409..>856)
                     /locus_tag="ACHK49_005373"
     CDS             complement(409..>856)
                     /locus_tag="ACHK49_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="secreted by salmonella pathogenicity island 1;
                     involved in survival of Salmonella in host cells;
                     interferes with host cell ubiquination pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFO9265801.1"
                     /translation="DYKMIADDQQRKLACVWNPFLDGWELNAQHVDMIMGSHVLKDMP
                     LRKQAEILFCLGGVFCKYSSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQVFGSVYY
                     YNDILDRLQGRNNVFTCTAVLTDMLTEHAKESFPEIFSLYYPVAWR"
BASE COUNT          315 a          192 c          104 g          245 t
ORIGIN      
        1 aattaaaatc atatttcatc tatagtgacc attcacagca aaacaaaatc aattatcaca
       61 ttcaaaaaaa atactgacaa caaagatact cttcagacaa agttgtcata tgtaaatttt
      121 cccttaaaac gttgctcaaa aaaaatataa aaatcatatg ccctaatcaa catcctcaag
      181 cactacgata tatgcagtat cctattcata tattaccacc atatcagaca aatcgaatca
      241 caacgagttc tatcgtactc tttcatcttc gacactgtaa attttaagaa cattcggctc
      301 atgccactaa accaagacat aataacaacg gagcaataaa tgttcccact gaacatgtgc
      361 aaacctaaaa agacagtgtt acaataatat tcatccatgg tctctaaatc aacgccacgc
      421 aacaggataa tacaatgaaa atatttcagg aaaagattct tttgcatgct ccgttagcat
      481 atcagtcagc acagcggtac aagtaaaaac attatttctt ccttgtagcc tgtctaaaat
      541 atcattgtaa taataaactg agccaaatac ctgaggatct gttttataag cttgttcaat
      601 caatccattt gcatatctcc gtagaatctc aggagaatca tactctgtac caaacatatc
      661 actcgatgag tatttacaga aaaccccccc taaacaaaat aatatttcag cctgttttct
      721 tagtggcata tctttcaata catggctccc cataatcata tctacatgct gagcgttcag
      781 ttcccaacca tcaagaaagg gattccagac acaggcaagt ttcctttgct gatcatcagc
      841 aatcatttta taatct
//
LOCUS       DAZGLJ010000571          855 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000571 DAZGLJ010000000
VERSION     DAZGLJ010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.584"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..670
                     /locus_tag="ACHK49_005374"
     CDS             <1..670
                     /locus_tag="ACHK49_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9265802.1"
                     /translation="QRADWQKGLQEGLSNWVDNASDYASQAAQLATEGISGMVNNITE
                     MLNGNKVEWRNWASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFIGSIGDWLGGAV
                     ANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVR
                     AVGSMNGSAGLVYSPVYHIAIQNDGANGQIGAEAAGSLVQLIDQRVQAVMLSMRRDGG
                     MLSG"
     gene            663..>855
                     /locus_tag="ACHK49_005375"
     CDS             663..>855
                     /locus_tag="ACHK49_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9265803.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEPTRRWLDE"
BASE COUNT          194 a          194 c          304 g          163 t
ORIGIN      
        1 gcagcgtgcg gactggcaga aaggcttgca ggaggggctc agtaactggg tggacaatgc
       61 atccgattac gcttcacagg cagcacagct tgcgacggag ggtatctcag ggatggtgaa
      121 taacatcacg gagatgctga acggaaacaa agtggaatgg cgcaactggg cctcatccgt
      181 actgcaggaa atctcaaaag ttcttatgaa tgccgcgatt gtcaacggaa ttaagacggc
      241 ggcaaacggt atgtccggtg cgggaggatt tattggcagc attggtgact ggctgggcgg
      301 tgcggtggcc aatgcaaaag gcggcgtgta tacctcggca aacctgagtg cgtacagcaa
      361 cagcattgtg gacacgccca cgtactttgc gtttgcaaaa ggggccgggc tgatggggga
      421 agccggacct gaagccatta tgcccctgac ccgggcggcg gatggctcgc tgggcgtacg
      481 cgcggtgggc agtatgaacg gcagtgccgg tctggtgtat tccccggtct accacatcgc
      541 cattcagaat gacggggcta acggacagat aggggcggag gcggcaggca gtcttgtgca
      601 gctgattgac cagcgggtgc aggcggtgat gctgtccatg cgacgtgacg gaggaatgct
      661 gagtggctga gataaaaacg ctgcatctgg tcccgcgtga agggatgcag gtgagtgaga
      721 aaccgtcggt ggtgagggtt cggtttggtg acggttatga acagcgccga ccgacgggac
      781 ttaatgccag actgaagacg tttcaggcgg tgttccgggt gacggatgaa ccaacccggc
      841 gctggctgga tgaat
//
LOCUS       DAZGLJ010000572          854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000572 DAZGLJ010000000
VERSION     DAZGLJ010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.585"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(393..>854)
                     /locus_tag="ACHK49_005376"
     CDS             complement(393..>854)
                     /locus_tag="ACHK49_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO9265804.1"
                     /translation="YILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREK
                     TEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVR
                     AQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDTVVSVARAVWNWIFF"
BASE COUNT          207 a          225 c          228 g          194 t
ORIGIN      
        1 ggtgttcaga tgtcgtttca tcagcttttc cttacagagg gtgaataaaa aagccggtac
       61 ccacaaatgt ggataccggc aggtacaaaa cagcatgctc agataaagtg gtttttctcg
      121 gacataaatg cttcccctct ctcatccgcc agagaagtat cagtccgttg tgtgcaattc
      181 cgttctgcat gatgaagaga ttcagctgcc ttttcgcaga ccattaacca ctataaacga
      241 tcgatagcaa cgatcgatac ctgttttatc gatcgtttta ttctattaac gacaaggcgg
      301 tcacgcaaga gaaacaggag gagtatgagg ggtttgtgtc atgttttcag ttggttaacg
      361 taatgttatc aggcatttcc ctggcagact gttcagaaga aaatccagtt ccatacagcg
      421 cgggcaacag agaccaccgt gtcacggacg gcacgcagaa ccgtacgggc aacagaggca
      481 acacagacgc tctccgccgt gtcaaatatc cggtccaccg caccggtaaa ctgttcacgg
      541 gcctgagcgc ggacagattc cgtgcgcagc tcgtcctgca ggcgggtcat caggggactg
      601 gcggcatggt cgggaagtgc tgtcattagc gcactgacca gcgtatccag ttcccagccg
      661 gtgcgggccg atacggcaac aaccggatgt acgggccgga acagacggaa taccgcctcc
      721 gttttctccc gaatgttctg tgcctgtgcg ggagaaggct gaatgccggc catatcccat
      781 tcatggcagg gctccgtttt gtcggcctgc gtcaccacaa acagcacctg ctgatgtccc
      841 cggtgcagga tgta
//
LOCUS       DAZGLJ010000573          851 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000573 DAZGLJ010000000
VERSION     DAZGLJ010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.586"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..131)
                     /locus_tag="ACHK49_005377"
     CDS             complement(<1..131)
                     /locus_tag="ACHK49_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO9265805.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWL"
     gene            173..358
                     /locus_tag="ACHK49_005378"
     CDS             173..358
                     /locus_tag="ACHK49_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO9265806.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLSTERSGMNYIIK"
     gene            complement(488..628)
                     /locus_tag="ACHK49_005379"
     CDS             complement(488..628)
                     /locus_tag="ACHK49_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265807.1"
                     /translation="MDFMLTVSGVVILSIAYTADKYGCHLLSRIGAYCSLMLIFSSLF
                     FE"
BASE COUNT          288 a          201 c          159 g          203 t
ORIGIN      
        1 ctgagccaga tatgacgctt gagggaaaca cgtgcactgc cactgacacg acgctgttca
       61 cccttcagaa tattcacccg tcgggtattc agtgttttga ttctgctctg gagtgtacga
      121 agctcagcca tgtaaaatcc ccgtcatatg gcaatcagta aaggaaataa atatgtcatc
      181 gaaaaaccgg acccgcagaa ccacaacccg caatatccgt ttccccaatc acatgattga
      241 acagatcaat atcgcccttg aacataaagg ttccggtaac ttttcagcgt gggttattga
      301 agcctgcaga agaaggctgt caacagagcg ttcgggtatg aattacataa ttaagtaaca
      361 tggtgttcac agaacacaca gttaccggac acatcagttt tccattcgtc ccccggcagt
      421 acaggcttcc cgtctgacgg gatagcctga aaaaacacag aaaattattt gttataatta
      481 atataactta ctcaaaaaaa agcgacgaga aaatcagcat caacgagcaa taagcgccaa
      541 tacgtgataa caaatggcag ccatatttat ctgcagtata agcaatggac aggataacca
      601 caccagaaac cgtcagcata aaatccattt gaacttcccc ggacaaaatc gactcatcta
      661 aagatttaca gctcttttta ttatcaatat gttaaaagta aaataaacag atgttcaata
      721 atacgaatac aaaaacgtgc tgaaaatcaa tgaatccatt tctgtgttat caattaatag
      781 tgataaacat ccggcttctt ccaccatcgc accggaccag cgaccatgag gggacaacgc
      841 cgcgctccgt t
//
LOCUS       DAZGLJ010000574          849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000574 DAZGLJ010000000
VERSION     DAZGLJ010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.587"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(48..568)
                     /locus_tag="ACHK49_005380"
                     /pseudo
     CDS             complement(48..568)
                     /locus_tag="ACHK49_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            612..692
                     /locus_tag="ACHK49_005381"
                     /pseudo
     CDS             612..692
                     /locus_tag="ACHK49_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          236 a          164 c          191 g          258 t
ORIGIN      
        1 ggtaaaatga ttcaggaaaa ccggttcagc atagcctagg ctgaactttt gaggatatca
       61 acgaacggaa tttttgaaca tttatgagta gatcgttgtt gaagggcttc aatgagcatg
      121 tcaagttcat caactgctgg tctacgatta cgacggtttg atttaccaat caaagcaagt
      181 ttaagtagat atggacgagc acttttcgcc gggtttgatg tgtaattaat tccgtatata
      241 ggtttggctg catccagaac actgccaaga taactaacat cgtgactgac tgttgctgga
      301 cctgcaccag cgttgtttct cagcctgcaa tgttcaatta cgtcattttc tgtcagttca
      361 gatagtttga tcgcggagat gtcactatcc ataagcagtt ctagcacata tcttttagta
      421 cggtctgctt tacctccggc atttggtcat ttaaatattt gtgtagtaag tcacggactg
      481 taagtccgtc aacagcattt gatgatgaaa tgccatatag atctaatacc atcactttct
      541 gtgtgcccca tgttttagca tgagcatgaa cgcggcggct atctcacggg tgatcatccc
      601 tttggcatac tgaaaagtat cgggtgttac tgaaccgtgt tgatacatca actgcactgg
      661 atattgagtg gccgataata ccgacgttat aacctataaa tgccaatcag gctgaatgtt
      721 gaaaggatag aatgaacagc ccgacacaca aaaaacggag tccggctccg gtttttgtgt
      781 tgccatataa ggcagatgtt tgttacagct atttaagtct ggagttcaaa ttaaaatagg
      841 gagttttgt
//
LOCUS       DAZGLJ010000575          845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000575 DAZGLJ010000000
VERSION     DAZGLJ010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.588"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..460
                     /gene="espL"
                     /locus_tag="ACHK49_005382"
     CDS             <1..460
                     /gene="espL"
                     /locus_tag="ACHK49_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE stator protein
                     EspL"
                     /protein_id="HFO9265808.1"
                     /translation="RKIMAMRERIRKNNKIKLEKEVNKSIKWVKDIQDIELVLMQDIM
                     NKVHSSLTNALHSLDTSSRINWDDLLNEVVRETLSHNNIVGAIKITKNPDIKLDPGEA
                     NNIQLINDADAPLNKIIIENEYMRITLDPLEQINILLNSFKENYLSIIQE"
     gene            465..>845
                     /gene="escR"
                     /locus_tag="ACHK49_005383"
     CDS             465..>845
                     /gene="escR"
                     /locus_tag="ACHK49_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFO9265809.1"
                     /translation="MSQLMTIGSQPIFLIIVFFLLSLLPIFVVIGTSFLKISIVLGIL
                     KNALGIQQVPPNMALTSVSLILTMFIMSPIILQINDNISQEPINYTDSDFFQKVDEKI
                     LSPYRGFLEKNTEKENVEFFERAAQ"
BASE COUNT          320 a          118 c          136 g          271 t
ORIGIN      
        1 cagaaaaata atggcaatga gagaaaggat tcgaaaaaat aataaaataa aattagagaa
       61 agaagttaac aaatcgataa aatgggttaa agatattcag gatatcgagt tggtcttaat
      121 gcaggacata atgaataagg ttcattcgtc actgactaat gcattacatt ctttagacac
      181 atcatcaaga attaattggg atgatttact taatgaagtc gtaagggaaa cgctttccca
      241 taacaatatt gttggagcta ttaagatcac taaaaaccca gacattaaac ttgatcctgg
      301 agaggctaat aatatacagc ttattaatga tgcagacgca ccacttaata agataataat
      361 agaaaatgaa tatatgagaa taactttaga tccgttagaa caaataaata ttttattgaa
      421 ttcatttaaa gagaattatc ttagcattat tcaggaatga tgatatgtct caattaatga
      481 ccattggctc acagccaata tttttaatta ttgtattttt tcttttatca ttactgccta
      541 tatttgttgt tattggtact tcattcctga aaatttcgat tgtattgggg attctgaaga
      601 acgcattagg tattcaacag gtaccaccaa atatggcgct aacatcagta tctttgatac
      661 tgacaatgtt tattatgtct ccgataatat tacagataaa tgataatatt tctcaggaac
      721 caatcaatta caccgactct gatttttttc aaaaagttga tgaaaaaata ttatcaccat
      781 accgcggttt tttagaaaaa aatacagaga aagaaaatgt tgagtttttt gaacgtgcag
      841 ctcaa
//
LOCUS       DAZGLJ010000576          845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000576 DAZGLJ010000000
VERSION     DAZGLJ010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.589"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>845
                     /locus_tag="ACHK49_005384"
     CDS             <1..>845
                     /locus_tag="ACHK49_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9265810.1"
                     /translation="GQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLDDKQQSALT
                     KTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAWRNNEANKP
                     KSRGGKSETEKAEDSFSRLLKQQKEQLALVGQNTELAKLKYQTAQGELKTLTEMQKQE
                     LLRNAALIDQQKIREQLRSREETLKNENAAARASNDAELLGYGQGERARERMRELQQI
                     RDSFRQKDADLQSQYQTGDISEDFYRQALSQNAQYLSERLKEQEAFYAESDVQRADWQ
                     KGLQEG"
BASE COUNT          232 a          187 c          283 g          143 t
ORIGIN      
        1 ggtcagcata cggaattcaa ccgtctgctg gggctgggta atgaactgct tcagcagcgg
       61 cagggactgg tgaatgtgcc gttacggctg ccacaggcca ctctggatga taaacagcag
      121 agtgccctga caaaaacaga gcgtgagctg gccctgtcca gactgaaagg ggaagaaaaa
      181 gagcgtgtcc ggctggggta tgcggcggat gacctcggtt ttgtgggtga tccgtatcag
      241 gaggcgagac agcgttatat cagtaatgcc ctggaagcct ggcgcaataa cgaggcgaat
      301 aaacccaaat cccggggtgg aaaatcagag acggaaaaag cggaagacag tttttcccgg
      361 ctgctgaagc agcagaaaga gcaactggca ctggtggggc agaatacaga gctggcgaag
      421 ctgaaatacc agactgcgca gggcgaactg aaaaccctga cggagatgca gaagcaggag
      481 ctgctgcgta atgcggccct gattgaccag caaaaaatcc gggaacagtt gcgatcccgg
      541 gaagagacac tgaagaatga gaatgcggct gcgcgtgcgt cgaatgatgc tgaactgctg
      601 gggtacgggc agggggaacg agccagagaa cgcatgcggg agttgcagca gatccgcgac
      661 agcttccgcc agaaggatgc ggaccttcag tctcagtatc agaccgggga tatcagtgag
      721 gatttttaca gacaggctct gtcgcagaat gcacagtatc tgagtgaacg tctgaaagag
      781 caggaagcct tttatgccga atcggatgtg cagcgtgcgg actggcagaa agggctgcag
      841 gaggg
//
LOCUS       DAZGLJ010000577          843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000577 DAZGLJ010000000
VERSION     DAZGLJ010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.590"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..425)
                     /locus_tag="ACHK49_005385"
     CDS             complement(<1..425)
                     /locus_tag="ACHK49_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO9265811.1"
                     /translation="MSQPVGDLIIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTATAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMLRGLAGAITLPMVGVTSLAVATGALAYAWYQG"
     gene            complement(406..>843)
                     /locus_tag="ACHK49_005386"
     CDS             complement(406..>843)
                     /locus_tag="ACHK49_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO9265812.1"
                     /translation="FALRLAREMGRPDWRAMLAGMTSTEYADWRHFYRTHYFQDTQLD
                     MHFSGLMYAVLSLFFCDPDMHPSDFSLLVPRHEEEQVERLDEDKMLMQKAAGLAGGVR
                     FGGDGGGDILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
BASE COUNT          192 a          279 c          212 g          160 t
ORIGIN      
        1 ccctggtacc aggcgtacgc cagcgcacct gtcgccaccg ccagcgaggt gaccccgacc
       61 atcggcaggg tgatcgcacc ggcaagcccc ctgagcatgg ggatcatccc gccgaaggag
      121 tccttcacct gaccgccctg ttgcagcagg atcagccagg gattctgacc accggcaagc
      181 tgcgtggcga tatccgtaaa ctgtgcgggc agggtgcgca tggccgcttt atactgtccg
      241 acggaaatcc cggctttttg cgcagccagc gcctgacggc tcaggccctg ttcaacagca
      301 gtggcggttt ttctggcgtc agtatccagt cctgaaaaat gacgccttac ccggctcatc
      361 tgctcatcga aacggaccgc atccagactc aggtcaataa taagatcacc aaccggctgg
      421 gacatatctc acacctcccg gaatccccgc tgaagccatc attaatgcgg catcatccac
      481 catgacatcc gccacatccg cagacgataa aatatcgccc cctccgtccc caccgaaccg
      541 gacgcctccg gcaagtcctg ccgctttctg catcagcatt ttgtcctcat ccagcctctc
      601 cacctgctct tcctcatgcc gggggacaag cagactgaaa tcagagggat gcatatccgg
      661 atcgcaaaaa aacaggctga gtacagcgta catcagcccg gaaaaatgca tatccagttg
      721 ggtatcctga aaataatgcg tgcggtaaaa atgtcgccag tcggcatatt cggtggatgt
      781 catcccggca agcatggcgc gccagtcagg cctccccatc tctcgcgcca gtctgagggc
      841 aaa
//
LOCUS       DAZGLJ010000578          842 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000578 DAZGLJ010000000
VERSION     DAZGLJ010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.591"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..547
                     /gene="espW"
                     /locus_tag="ACHK49_005387"
     CDS             <1..547
                     /gene="espW"
                     /locus_tag="ACHK49_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFO9265813.1"
                     /translation="PSEKKEMWIYKNNGKDNAKPNLGERDVRMFENISPDDTDKITGR
                     KFSELGEYLYSGNVIKLSQLSIRYLPNINSISLIETKQSLLLHRLYSDEVLQRNGTLI
                     PTPLHEEKSIPADNIKTMLNNIPTYKMLPPFTETQGNCSSGAATFLRKSGAEEKDILA
                     CSPRNYGLHHNIKTWDPLVRN"
     gene            complement(675..>842)
                     /locus_tag="ACHK49_005388"
     CDS             complement(675..>842)
                     /locus_tag="ACHK49_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO9265814.1"
                     /translation="GAEICSLYDKDALVQLVETGGAHPLSREPITESMIMRKDECHFD
                     SKKEAFVASDA"
BASE COUNT          322 a          159 c          135 g          226 t
ORIGIN      
        1 tccttcagaa aaaaaagaga tgtggatata taaaaacaat ggaaaagata atgcaaaacc
       61 aaacttagga gagcgagacg taagaatgtt cgaaaatatt agtcctgatg atacagataa
      121 gataacagga aggaaatttt cagagttagg tgagtatctt tattcaggaa atgtaataaa
      181 actcagtcag ttatcaattc gttacttacc caatatcaac tcaatctcat taatagagac
      241 aaaacagagt ttgttgctac atcgattata ttcagatgaa gtacttcaga gaaatggaac
      301 acttatcccg acaccactac atgaagaaaa atcaattcca gctgacaata taaaaacaat
      361 gctcaacaac ataccgactt acaaaatgtt accgccattc acagaaacac aaggtaattg
      421 ttcttctggc gcagccacgt ttttacgcaa atcaggcgcc gaagaaaaag atattcttgc
      481 atgtagcccc cgaaattatg ggctgcatca taacataaaa acatgggacc ccttggttag
      541 aaattaagga tccaaatatt atatatttca tataaagcaa ggaaaatatt gcctgaataa
      601 ttgttcaggc aatatatcct tacatggcac tattataata aactattaat ataaaaacac
      661 accaacagaa aaaattaagc atcacttgca acaaaggctt ctttttttga atcaaagtga
      721 cattcgtctt ttctcatgat cattgattct gttataggtt ctcgactcag aggatgagct
      781 ccaccagttt caacaagttg cactaacgcg tccttatcat atagagagca tatctcagca
      841 cc
//
LOCUS       DAZGLJ010000579          838 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000579 DAZGLJ010000000
VERSION     DAZGLJ010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.592"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(251..418)
                     /locus_tag="ACHK49_005389"
     CDS             complement(251..418)
                     /locus_tag="ACHK49_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO9265815.1"
                     /translation="MTVKLRLTVAALLLFLVVMVDFTSRIMSVLADGVLVCGIMVLLW
                     PVIKRNSLHNA"
     gene            complement(415..838)
                     /locus_tag="ACHK49_005390"
                     /pseudo
     CDS             complement(415..838)
                     /locus_tag="ACHK49_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310776.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
BASE COUNT          219 a          248 c          172 g          199 t
ORIGIN      
        1 ccatctgcca gtggctggtc gtaaaaaaaa ggccgcgcca tgcgcagccg aaaataaagg
       61 gataacgatg atagtttgag aaaaacagaa ataacacttt tgtggcaaag catggtgccg
      121 ggtgcctccc ggtgaattca gtatcagcac ctgaatccgc gattacccca tattccttct
      181 tgctgattgc cccaccgcac agggggattc accatgcaga agtgttttta ataaacagca
      241 aacaaaaaaa tcaagcatta tgcaggctgt ttctttttat caccggccac agcaatacca
      301 taatgccgca gaccagcacc ccatccgcca gcaccgacat gattctgctg gtgaaatcca
      361 ccatcaccac cagaaacagc aggagtgcag ccacagtcag gcgcagtttt accgtcacag
      421 gtaattctcc agacgaagac ccagaacacc ggcaatctct tccagcacct tgcgctcttc
      481 cggctcaatt tcgccgtctg cctccgcaat ggccaccgcc acatccagca catcttccgc
      541 ttcacgcgta tcgtgtttca catcctcgat ctcacgtaac gccgcacgac gaccagtttt
      601 aaagttcgta tccagctgac cgataatggt tgcgctaatc gcattaattt ctgacgtaaa
      661 cgcgtacagc gcaggctgat tacgcagtac ctgttcgatc ttcgctttct aggaagcctc
      721 acattcacca tctgcatagg ccaccaggta ggcggcgtta atcaccacct gtgccagatc
      781 gcgtttttca aactttttaa tttccgttgc cgctctgcgg gcttttttta ccaaaaat
//
LOCUS       DAZGLJ010000580          834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000580 DAZGLJ010000000
VERSION     DAZGLJ010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.593"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(89..403)
                     /locus_tag="ACHK49_005391"
     CDS             complement(89..403)
                     /locus_tag="ACHK49_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO9265816.1"
                     /translation="MLHDHLAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     KC"
BASE COUNT          252 a          208 c          118 g          256 t
ORIGIN      
        1 tacttaaacg acttatatat gacacaaaaa gcgaccacta aagtcgcttt ttcttatggt
       61 aacaggcaat aactctctca gatatttttt agcatttttt tgaccgcgcg tttccggacg
      121 tattctgttc tcctgtccct ttatatcgtc ggaatacccg ccgctcttca aatcccattc
      181 ccaactcaga atgtagtctg ttgaccgctt gttttatttc ggtcaggttc accggtgaaa
      241 ccggagtccg gcgcgcctta cgcaaacact ctgctcgttt ctgtgccgcc acttttcttt
      301 tctggtcatc acttagctgt accatcactt ttgcccatcg ttcagctgct ctccggtaca
      361 gtcctttttt ctccagacat tctgccaggt gatcatgtag cataagtaac ctccgtttat
      421 ctacagactg ccatcctgaa tttaccgtcc ttaataagac aacaataaaa aactaattat
      481 ataaaaacaa taaaaataat atacaaaaaa actaaacaac aaagaaaagc aaatcatatc
      541 tggcatttta attgaaagag caattaccga acaaaagacg ctgactatat actcaaaacc
      601 aaacaacaat tctgccaatc aggtatcatg gcaacacacg gaattaccat gtttttgcct
      661 tctctgccca tacaatacgg gcatatactt catactctat tgtaatattt ctatccatgc
      721 gccccactcc atttacctgt aaataatatt caaaatattt atcacagaaa tcgtttttgg
      781 ccatgaactg agcacactat aaagtccgga actgactctt tgttaaatta cctt
//
LOCUS       DAZGLJ010000581          834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000581 DAZGLJ010000000
VERSION     DAZGLJ010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.594"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..746
                     /locus_tag="ACHK49_005392"
     CDS             <1..746
                     /locus_tag="ACHK49_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HFO9265817.1"
                     /translation="VSVINPALGKAFAQSEGLRNKTDTVDARMLAEFCRQKRPAAWEA
                     PHPLERALRALVVRHQALTDMHTQELNRTETAREVQRPSIDAHLLWLEAELKRLEKQI
                     KDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGLKDRFAHARQFAAFAGLTPRRY
                     ESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEWGRAFRDRLAANGKKGKVILGA
                     MMRKLAQVAYGVLKSGVPFDASRHNPVAA"
BASE COUNT          204 a          205 c          267 g          158 t
ORIGIN      
        1 tagtgtcagt cattaatcct gcgctgggta aagctttcgc tcagagtgaa ggactgcgta
       61 acaagactga taccgtggat gcgcgcatgc tggcagagtt ctgtcgtcag aagcgccctg
      121 cagcctggga agcgcctcac ccgcttgaac gcgcgttgcg tgccctggta gtccgccacc
      181 aggcgctgac agatatgcac acgcaggaac tgaatcgcac tgaaacggcg cgggaagtcc
      241 agagaccgag cattgatgct caccttctgt ggcttgaagc agagctgaag cgtcttgaga
      301 agcagataaa agacctgaca gacgatgatc cggatatgaa acaccgcagg aaactgctgg
      361 aaagcatccc gggtatcgga gagaaaacat ctgcggtatt gctggcttat atcggtctga
      421 aggaccgctt cgcccatgcc agacagttcg ccgcttttgc gggtctgaca ccacggcgtt
      481 atgaatcagg tagcagtgtg agaggggcga gccggatgag taaggccgga catgtgtcgc
      541 ttcgcagggc gttgtatatg cccgcaatgg tagccaccag taagactgag tggggacggg
      601 cgttccgcga ccgtctggcg gctaatggca agaaaggaaa ggtgattctc ggcgcgatga
      661 tgcgcaagct ggcacaggtg gcgtatggag tgctgaagtc aggcgtgccg ttcgatgcgt
      721 cacggcataa tccggtagcg gcgtaaaaat cgcggaaggg atgaaaaaaa cagcgcctga
      781 cggcgctgtg tctggcatgc ctgcaatccg ggaaaccgga ccaggaaaaa actt
//
LOCUS       DAZGLJ010000582          830 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000582 DAZGLJ010000000
VERSION     DAZGLJ010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.595"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>830
                     /locus_tag="ACHK49_005393"
     CDS             <1..>830
                     /locus_tag="ACHK49_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9265818.1"
                     /translation="KGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQSIL
                     VNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTRTI
                     TSANIDRLRVTFGVQSLVQTTSKGDRNPTSVRLLIQLQRNGNWVTEKDVTINGKTTSQ
                     FLASVILDNLPPRPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIVGL
                     QVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWD
                     "
BASE COUNT          204 a          226 c          258 g          142 t
ORIGIN      
        1 aaaggtggcg gcagggcgca cacacctcgt gaggcgaagg acaatctcaa atccacgcag
       61 atgatgagcg tgattgacgc cattggtgaa gggccgattg aaggtccggt gaaggggctg
      121 cagagtatcc tggtgaacaa aaccccgctg acggacacgg acggtaatcc tgtgatacat
      181 ggtgtgaccg cggtctggcg cgccggggag caggagcaga caccacctga aggctttgag
      241 tcctccgggg cggaaaccgc actgggcgtg gaagtgacga aggcaaagcc ggtgacgcgc
      301 accattacat ccgcgaacat tgaccgcctg cgggtcacct tcggggtgca gtcactggtg
      361 cagaccacgt caaagggtga ccgaaacccg acatccgtcc gactgctgat tcagttacag
      421 cgtaacggta actgggtgac ggaaaaggat gtcaccatta acggcaagac cacctcgcag
      481 tttctggcgt cggtgattct ggataatctg cctccccggc cctttaacat ccggatggtc
      541 cgggagacag cggacagcac ctcggaccag ctgcagaata agacgctctg gtcgtcatac
      601 accgaaatca tcgatgtgaa acagtgctac ccgaacacgg cgattgtggg gctgcaggtg
      661 gatgcggagc agttcggcgg ccagcagatg acggtgaact accatatccg tggtcgcatc
      721 attcaggtac cgtcaaacta tgacccggaa aaacgcacgt acagcggtat ctgggacggc
      781 agcctgaaac cggcatacag caacaacccg gcctggtgcc tgtgggacat
//
LOCUS       DAZGLJ010000583          825 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000583 DAZGLJ010000000
VERSION     DAZGLJ010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.596"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>825
                     /locus_tag="ACHK49_005394"
     CDS             <1..>825
                     /locus_tag="ACHK49_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265819.1"
                     /translation="IGINFKDLPGPLKNALSNSNIPTTVSTAASTIASATTSTVTTAS
                     KDPIPWFGLTAQVVRNHGVELPIVKTENGWKLVGETPLTPDGPKANYTEEWVIRPGEA
                     DFKYGASPLQATLGLEFGAHFKWDLDNPNTKYAVLTNAAANALGAVGGFAVSRFTGTD
                     PMLSPHIGAMVGQAAGHAIQYNTPGLKPDTILWWAGTTLGLADLNKAETGEGRFTDYP
                     RIWWHAREGAIFPNKEDIARATGADIRAMEEGIPVGQRHPNPEDVVIDIESNGSPH"
BASE COUNT          249 a          181 c          194 g          201 t
ORIGIN      
        1 aataggtatt aattttaaag accttcctgg tccattaaaa aatgcattaa gcaacagcaa
       61 tataccaacc actgtatcga ctgctgcatc cactattgca tcagccacta cttcgacggt
      121 aaccaccgcg tcaaaagacc caataccatg gtttggatta acagctcaag tagttcgtaa
      181 tcatggtgtg gaacttccta tagtcaaaac tgaaaatgga tggaagcttg ttggagaaac
      241 tcctcttact cctgatggcc ccaaagcaaa ttatactgaa gagtgggtga tcagaccggg
      301 agaagcagat tttaaatatg gtgcatctcc actacaggca actctagggc tggagtttgg
      361 cgcacatttc aagtgggatt tagataaccc taatactaaa tatgccgttc ttaccaatgc
      421 tgccgcaaat gcgcttggtg ctgtaggggg atttgcagta tccagattta ctggtacaga
      481 tccaatgtta agtcctcata tcggtgcaat ggttgggcaa gcagcggggc atgccataca
      541 gtataatacc cccggattaa agccagacac tattttatgg tgggcaggta cgactctggg
      601 gctggcagat ttaaacaaag ccgagacagg tgaaggcaga ttcactgact atcctcgtat
      661 atggtggcac gcaagagaag gtgccatctt ccctaataaa gaagatattg cccgtgcaac
      721 aggggctgat atacgagcta tggaagaagg tatacccgtt ggacagcgac atccaaatcc
      781 agaggatgtg gtcatcgata tcgaaagcaa tggctcacca catca
//
LOCUS       DAZGLJ010000584          822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000584 DAZGLJ010000000
VERSION     DAZGLJ010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.597"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..501)
                     /gene="yhaB"
                     /locus_tag="ACHK49_005395"
     CDS             complement(<1..501)
                     /gene="yhaB"
                     /locus_tag="ACHK49_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFO9265820.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPLVHFNKTSIT
                     SDCWQIL"
     gene            complement(757..>822)
                     /locus_tag="ACHK49_005396"
     CDS             complement(757..>822)
                     /locus_tag="ACHK49_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFO9265821.1"
                     /translation="LINVNFFSSDRTSFCECNRFP"
BASE COUNT          241 a          157 c          161 g          263 t
ORIGIN      
        1 gagaatttgc caacaatctg aagtaatgct ggttttatta aagtgcacaa ggggtgttaa
       61 gatggcttct tttcgggtga attttgcaca atacttagaa atcgtctcct taatacattc
      121 cagctctttt ttattgacgg ctgagcaagt acattgttcg ccaaaaggtg caaaagtgcc
      181 tacatctaat gtttgtatga tataatcatc atgttcccct gtagaaatat ctatgatgaa
      241 tggactcata ttttccggtt ggattcggat cattacagaa ggaacatcgc cttgatagaa
      301 attcaatgca gtaattgcag ataatactct ccgcagttca ttacttatac tgtaaatttt
      361 attaacgtca ttggtggaga caatttctgc aaactttctt tttaccgtcc aataatcaat
      421 atttctattg tgattgttaa ccactgcgtg cattggcggg attgaagggt gaaaaatatg
      481 tgcaattggg aagcctttca ttgatgcctg ccttgtcgtc atgacaaaca ataatagatg
      541 cttattttcc tcgcagggtt ctttcgcact atcaaaaaac gctcatttac ccggcgttac
      601 agtaaacgaa gcaacattct gtggcctacg acgatcaaaa gctctgtgaa aatttccctt
      661 gttgtctttg tctgacctta tcgcggcgac cagcaattat tcgttttgtg atcctcaaca
      721 caaaatgcta ttcgtcgcct atgaggggag aagaatttat ggaaaacgat tgcattcaca
      781 aaatgacgtc ctgtcagaag agaaaaagtt gacgttaatc aa
//
LOCUS       DAZGLJ010000585          819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000585 DAZGLJ010000000
VERSION     DAZGLJ010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.598"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(185..757)
                     /gene="lpfA-O113"
                     /locus_tag="ACHK49_005397"
     CDS             complement(185..757)
                     /gene="lpfA-O113"
                     /locus_tag="ACHK49_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFO9265822.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          237 a          199 c          164 g          219 t
ORIGIN      
        1 acccgcgtac caccaatgac aacgcctgca taagttaatg ctggttggcc taaaaaaacg
       61 ctgagtataa tcgcggtaaa taacttacat gaaactgttg ttttaaagag cctgattaaa
      121 caactttttc tggacataac gactccctgg ccaaagccaa ctgaataaaa aggcccttga
      181 taaattattt cttatattcg acagtaaatt gtgaatcagc gttcgccgta ccagccgtta
      241 cggtcgcatt ggttgccaca tagcgtgcaa taaacttcat ttcagccttg ccttcagcat
      301 cgatagcggt ataagcagag tcgttataaa gtttaacctg cgaattatcg gactggttaa
      361 aaatacgaat accaacgccg gttgccgcaa tcgcttgtcc tgtaccggta aaatcaccag
      421 ggttgcctgc tgtaagcggc gtacccactt tcaggtcgcc gtcgccgcca ggcgcttctg
      481 taccatcaaa acgaacggct gcttgagtgt acgttgccgg gcaattttca agcttaatac
      541 tgaacttctg tggcgacgcc agactaccta cacctgaaaa tgcattttta ctgactttac
      601 ccagtgggac aatttgatcg gctgtatcgg aggtaacttc acaagtagtg tcgacaattt
      661 caccgacgaa gtgaacaaca ccatcttctg ccagtgcatg cccggccagc attagagatg
      721 ccagagtgaa tgcaccgcct ataatattac gcttcataaa aactccattg atataatata
      781 tcctttacca tgaatcatca cttatattaa taagtgtat
//
LOCUS       DAZGLJ010000586          805 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000586 DAZGLJ010000000
VERSION     DAZGLJ010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.599"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..694
                     /locus_tag="ACHK49_005398"
     CDS             <1..694
                     /locus_tag="ACHK49_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO9265823.1"
                     /translation="QLPNTVNLSSLTYNKEDDFLYATQNSPAQLLKIARSGDIIGRAP
                     LPFISDAETIEHIQGNIFAAVDEKTSELFFFTVTKDMHISLRNKIQLEKFNKKNRGFE
                     GLAWKAEGRMLFVAKERRPTGMFAYQLSPDLLSAKQVTIPEELNDIHVKDISGLDFNN
                     ESLMILSDESRKLLKFNLTEMSFVEMMDLTKGNHSLTSDLLQPEGIVTLSDESIYVAS
                     EPDILAKFVPNK"
BASE COUNT          269 a          162 c          152 g          222 t
ORIGIN      
        1 acaattacca aatacggtga acttatcatc tcttacgtat aataaagagg atgatttcct
       61 gtatgccacc cagaatagcc ctgcgcagct acttaaaata gccagaagtg gcgacatcat
      121 cggccgagcc ccgctgcctt ttatctctga tgccgaaacc attgagcaca tacaagggaa
      181 catttttgct gcagtagacg aaaagacttc tgaactattc ttttttacag ttacaaaaga
      241 tatgcatatc tccttgcgaa ataaaattca gttagaaaaa ttcaataaaa aaaatagagg
      301 gtttgaagga ctggcatgga aagcagaagg ccggatgctg tttgtcgcta aagaacgacg
      361 ccctacaggc atgtttgcct atcagctttc tccagatctg ctgagtgcaa aacaagtaac
      421 gattccagag gaactaaatg atatacatgt taaagatata tccggcctgg actttaataa
      481 tgaaagtctg atgattctgt ccgatgaatc gcgtaagtta ctcaagttta acctgacaga
      541 aatgtctttc gttgagatga tggacctgac aaaaggaaat cattctctga ccagtgattt
      601 gctccaacct gaaggcattg taactctgtc tgatgaaagc atttatgtcg ctagtgaacc
      661 tgatatcctc gctaaatttg tccctaataa ataagtctgt cactagcctt agtattgagc
      721 gaccaaatct ggcaatctca tgaaactcgc aaaatacgaa tctaatatta ataaaaattt
      781 tactctgaaa taaacagatt aatag
//
LOCUS       DAZGLJ010000587          805 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000587 DAZGLJ010000000
VERSION     DAZGLJ010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.600"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            361..>805
                     /gene="alaE"
                     /locus_tag="ACHK49_005399"
     CDS             361..>805
                     /gene="alaE"
                     /locus_tag="ACHK49_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO9265824.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPANILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVK"
BASE COUNT          220 a          171 c          174 g          240 t
ORIGIN      
        1 tgttaatgta cattttctgg cataaaaaat gctgtaaatg acccggtcac gacatacacc
       61 gaattctcta cgcttatagc gttacctcac ccccaaactt tcattagtga aattaaaggc
      121 aaggtcatat ccaattgatt taattgaaaa taaatcatat cggcatgtaa aaatccacag
      181 tatacaaaaa acgagccgtt acggaatatt ttatctacaa aaactgacta aataaaaatt
      241 tttcactaat tgaatagtca tagccagcga tatacgctat gcgaaaatgc agatggcaat
      301 gagatccact gctttcatct ccattaacat cccattacgc ttttattaag gagcattagc
      361 atgttctcac cgcagtcacg cttgcgtcat gcagttgcag atacgttcgc gatggttgtt
      421 tactgttctg tcgtgaacat gtgtattgaa gttttcctct ccggaatgag cttcgaacag
      481 tctttttatt ccagattggt agcgattccg gcgaacatct taattgcatg gccatacggt
      541 atgtaccgcg atctgtttat gcgcgcggca cgcaaagtta gcccgtcggg ctggataaaa
      601 aatctggctg atatcctggc ttatgtgacg ttccagtcac cggtgtatgt ggcgatcttg
      661 ttagtggtgg gcgcagactg gcatcagatt atggcggcgg tcagttcaaa catcgttgtt
      721 tcgatgttga tgggggcggt ttatggctac ttcctcgatt attgccgccg actgtttaaa
      781 gtcagccgtt accagcaggt aaaag
//
LOCUS       DAZGLJ010000588          801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000588 DAZGLJ010000000
VERSION     DAZGLJ010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.601"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>801
                     /locus_tag="ACHK49_005400"
     CDS             <1..>801
                     /locus_tag="ACHK49_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO9265825.1"
                     /translation="QRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTGLKI
                     SGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLKAMA
                     DDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASGVTK
                     TLSGMLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAVSATAGLVTAARNEVALAE
                     AQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNR"
BASE COUNT          172 a          191 c          286 g          152 t
ORIGIN      
        1 cagcggacgg gcacggcatt ttcagataat gcggccctgt ttgcccgctc ggcagcctca
       61 atgcgtgaat atggttacag tgctgatgat gtgctgaagg tgacggaggc catttcgaca
      121 gggctgaaaa tctccggtgc cagtacggca gaggcgggct cggtgatcac ccagttcagt
      181 caggcgctgg cgcagggtgt attgcgtggt gaggagttta attcggtcaa tgaaagtggt
      241 gaccggatcg tacgtgcact ggctgcgggt atgggcgtgg cccgtaaaga tctgaaggca
      301 atggcggatg acggaaaact gacagcggat aaagtggtcc ccgcgttaat cagccagctg
      361 gggatattac gtgatgaata tgcggccatg ccggaaacgg tttccagtag tatcacaaag
      421 gtggaaaacg cctttatggc ctgggtgggc ggtgcgaatg aggccagcgg ggtgacaaaa
      481 acgctctccg gcatgctgaa cggtgttgcc ggacagattg ataatgtggc aacagccgtg
      541 ggcgcgctgg ttgccgtcgg ggttgcccgg tactttggca atatggcctc cggagcggtg
      601 tctgccacgg caggacttgt gacggcagca cgtaatgaag tggcactggc ggaagcacag
      661 ttcaggggaa cgcagattgc cacggcgcgg gcaagggcag ccgtgtaccg tgctcagcag
      721 gccgtggcgg cagcccgcgg gacggagatg cagattgctg cagaggcccg tctggcggcc
      781 acacaggaac gcctgaacag a
//
LOCUS       DAZGLJ010000589          799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000589 DAZGLJ010000000
VERSION     DAZGLJ010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.602"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            40..315
                     /gene="yddL"
                     /locus_tag="ACHK49_005401"
                     /pseudo
     CDS             40..315
                     /gene="yddL"
                     /locus_tag="ACHK49_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            354..>799
                     /locus_tag="ACHK49_005402"
     CDS             354..>799
                     /locus_tag="ACHK49_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO9265826.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNVINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQG"
BASE COUNT          286 a          158 c          136 g          219 t
ORIGIN      
        1 agaaaatacg tttaacgata attaaaagga ttctgttaaa tgaaattaaa aatagttgcg
       61 gtggttgtaa ctggtttgtt agctgcgaac gtagcacacg ctgccgaagt ctataacaag
      121 gatggtaata aactcgacct ttatggcaag gttaccgctc tacgttattt tactgatgat
      181 aagcgtgacg atggtgataa aacttatgcc cgtctcggct ttaaaggaga aacgcaaatc
      241 aatgatcaaa tgattggttt tggtcactgg gaatatgatt ttaaaggcta taacgatgaa
      301 gccaacggct cgcgcggcaa gaatctctga ttgagttaag tgcccatcac aatatgatca
      361 ctgaccttat attacacaat catcccagga tgaaaacaat cactttaaac gacaaccata
      421 ttgcacattt aaacgccaaa aacactacaa aactggaata tttaaactta agcaataaca
      481 atttactgcc aaccaatgac attgatcaac taatatcatc aaaacatctt tggcatgtat
      541 tagttaacgt catcaacaat gatccacttg cacaaatgca gtactggact gcagtaagaa
      601 atataattga tgacactaat gaagtgacca ttgatttatc aggacttaat ttaaccactc
      661 aaccaccagg gctgcaaaac ttcacctcta tcaatcttga taataaccaa ctcacacatt
      721 ttgatgcaac caactacgat agactcgtaa agctaagtct gaatagtaat gctcttgagt
      781 caataaattt tcctcaagg
//
LOCUS       DAZGLJ010000590          799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000590 DAZGLJ010000000
VERSION     DAZGLJ010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.603"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..183)
                     /locus_tag="ACHK49_005403"
     CDS             complement(<1..183)
                     /locus_tag="ACHK49_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO9265827.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSD"
     gene            complement(183..>799)
                     /locus_tag="ACHK49_005404"
     CDS             complement(183..>799)
                     /locus_tag="ACHK49_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265828.1"
                     /translation="FDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTNAAGNHSHRDGRRFN
                     PSVFKDTYQYGYTSSGQNTWGVQGSVGMSTGWLANTSTDGNHSHSLSGTAASAGAHAH
                     TVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT          196 a          189 c          192 g          222 t
ORIGIN      
        1 atcactgttg aaaacagcca caaagccagc aggaatatct ggtggtgcaa tatcggtact
       61 gtttgctggc agacctgtat gaggcggaat atatgcgtca ccttcaccaa taaattcatt
      121 agttccggcc agcagattat aaatttttat ggtccgtggt tgttcactca ttctgaatgc
      181 cattatgcaa gcctcacaat atagttaaat gcgatgtttt tgacggtgtt ttccgcgtta
      241 ccagcagcgt taacggtgat ggtgtgtcca tgtgaaccaa tcgcaacgga gtgcgtatga
      301 gcaccaatac cgacagtatg cgcgtgtgca cctgcagatg ctgctgtgcc ggacagtgaa
      361 tggctatgat taccatctgt actggtattc gctaaccacc ccgtagacat acctactgag
      421 ccttgtacac cccaggtatt ttgacctgag cttgtataac catattgata agtatcttta
      481 aaaacactgg ggttaaatcg acggccatct ctatggctgt gattaccagc tgcattcgtg
      541 ctgccactta aactatgggt atgcgcacca gtgttattcg tggatttagt gccgtaatca
      601 aacgacgatg tggttttcgt ccccaaatcc gtactggatg cgctggcgct gtgggtatgc
      661 gatttaatgc cgtcctgttc ctgagacaat acggcccgac cactggcagg tttgccctta
      721 atcgtccagc cacgcatatc agggatcacg cctgacggat aagccgctgc aagtttcggg
      781 tatgcagatt tgtcaaaag
//
LOCUS       DAZGLJ010000591          797 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000591 DAZGLJ010000000
VERSION     DAZGLJ010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.604"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(20..691)
                     /gene="queE"
                     /locus_tag="ACHK49_005405"
     CDS             complement(20..691)
                     /gene="queE"
                     /locus_tag="ACHK49_005405"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO9265829.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          199 a          210 c          178 g          210 t
ORIGIN      
        1 aacgcttata aatgtttaat caggcaatat ttagatattt atgtgtttgc atcgacaaac
       61 gccaattacg cgcaatgcag gtttcaatgc acaaacgtgt ggcatcatcc ttttggctaa
      121 tcggctgcag tgcaatgact cgcggtttat catcggtcag cgttgccagt agttcatcca
      181 gtgcttcaat atcacgtacg cgccccactg gatgcttgat ttcgttggct cgctccagtg
      241 cctgtgacaa cacgtcatag ccgccgcgca tgttcagctt tggcgatacg gtaacccagg
      301 tattcggtgt gcagcgtact tcatgagttc cactggtttc gatctggcag ctaaaaccgt
      361 tcttttcgag caggtcagtc agtggcagca aatcatgaat gcaaggctca ccacccgtaa
      421 tcaccacatg ccgcgcggta tatccctgcc gaccaatgac agccagcaaa tcttcactgc
      481 tcgccgcccc ccacttatca ctctctttgg tcttcgccag aatgctgaaa agggagactt
      541 cccgatcctc aagcttttcc caggtgtgtt tggtgtcgca ccaggcacag ccaaccgggc
      601 atccctgtaa acgaataaaa atggcgggga cgccggtaaa gtaaccctca ccttgcaggg
      661 tctggaacat ctcgttaatc gggtactgca tagcattctc tgtgaagtgg ataattgtta
      721 attattgcag atcctgccac aacaatcatg tcttattaac attctgttac aggcagattt
      781 aaagaaaaat gcgaaac
//
LOCUS       DAZGLJ010000592          796 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000592 DAZGLJ010000000
VERSION     DAZGLJ010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.605"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            376..483
                     /locus_tag="ACHK49_005406"
     CDS             376..483
                     /locus_tag="ACHK49_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO9265830.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT          203 a          186 c          246 g          161 t
ORIGIN      
        1 cgtgtcatgt gggcgtcagg ctgccgtaat ggcaatttgc gcccggacca ggccgcagga
       61 gtgaaactct gcggcctttt tcgtaagaaa aatgcaacgg ttgcaagcgg ataatattct
      121 gcggaatgcg cagcacagag tgaagatgag acggattccg gaacagaaaa acagtcgtag
      181 aatcagcgtc aagccggaag gattccggtg aggcgcaatg ttgcgggggc ttaatccctg
      241 gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg caggtatgca cctgacaaca
      301 ccacgggggc cattcttgca tccaaacaat acaagaatag ccgcgaaacg ttgtcattac
      361 aatacaggcg gctatatgac gctcgcagaa ctgggcatgg ccttctggca tgatttagcg
      421 gctccggtca ttgctggtat tcttgccagt atgatcgtga actggctgaa caagcggaag
      481 taacgtgcca tgcgggcgtc aggctgccgt aatggcaatt tgcgcccgga tcaggccgca
      541 ggggtgaaac tctgcggcct ttttcgtaag aaaaatgcaa cggttgcagg cggataatat
      601 tctgcggaat gcgcagcaca gagtgaagac gtgacggatt ccggaacaga aaaacagtcg
      661 tagaatcagc gtcaagccgg aagggttccg gtgaggcgca atgttgcggg ggctttatcc
      721 ctggtggcat tggttgctgg agagagaaaa cccccgcacg ttgcaggtat gcacctgaca
      781 acatcacggg ggccat
//
LOCUS       DAZGLJ010000593          793 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000593 DAZGLJ010000000
VERSION     DAZGLJ010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.606"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          268 a          177 c          138 g          210 t
ORIGIN      
        1 cttataaaag ttattaatca ccgatgtata atgtgtaaaa cacattatca ttaaatgtca
       61 acaaaaaaat catatttctt aaaaatcatt caaaatcaat aaagtagaag aacttcatct
      121 ctttagcaaa cgccattaac aattgccttt ccatttagtt aatcgttttg ttttctttaa
      181 gattagtcct attagacaaa tagcaaaatg gaataactac agcactaaga cgttccgcta
      241 tatagctatt acctgttccc ctgacataag gagcagatat acctcaaccc cctattcccc
      301 agtgctgacc gggagcggcg gcggtataaa aaggaacaag gaacaaatgg cagaacaagc
      361 aaccatgttt gtcccccccg cataaccgta ccattcgcat taagaaatat tcccatatct
      421 atcccgcaaa agaggacccg tcatacatca agtccgtttt actgggcatc agattatcgc
      481 cgtaattagc tcagtgaaag ccgggtaaac ggttagcaag ctcagtggct caaaagtgca
      541 tagcgctaag atgggcctgg ttacacctgc cctttgctgc agatcgtgtt gatcggtgtg
      601 atgccgccac acgtttaagt caatctggaa aactcacaat gcgctcttct ataaagagga
      661 ctctttgata aagtaacccg aaaagggaaa gctaactgat atcaatgtac caccttagta
      721 atcatccctt atatacagac aaaaagaagt tgcctgaaag ccaggtatct aaactaatca
      781 ctaatggtat att
//
LOCUS       DAZGLJ010000594          788 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000594 DAZGLJ010000000
VERSION     DAZGLJ010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.607"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            28..>788
                     /locus_tag="ACHK49_005407"
     CDS             28..>788
                     /locus_tag="ACHK49_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265831.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMV"
BASE COUNT          253 a          164 c          179 g          192 t
ORIGIN      
        1 ttatgacagg cgagggagtg tccaattatg aattcaaaaa agctttgttg catatgtgtg
       61 ttattctcgc tgcttgcagg atgtgcctct gaatcttcta ttgatgaaaa gaagaaaaaa
      121 gctcaagtca cacaaagtaa tattaataaa aacactcccc agcaactgac agacaaagat
      181 ttattcggta atgaaaccac tctggccgta tccgaagagg atattcaagc tgcgcttgat
      241 ggagatgagt tccgcgttcc ccttaattct cctgtaatac ttgttcaatc cggcaaccgc
      301 gcaccggaaa ccattatgca ggaggagatg cgaaaatatt atactgtttc cacattctct
      361 ggtatcccgg acaggcaaaa gcctctgact tgtaacaaaa acaaagataa aaatgagaac
      421 gaggatgttg ccagtgctga gaatatgaac tggatgcagg cactgcgttt tgtggctgca
      481 aaagggcatc agaaagcgat tattgttttc caggatacgt tgcagacagg aaaatatgac
      541 tctgcgctga aatcaacagt atggtccgac tataaaaatg aaaaactcac cgacgctatc
      601 tccctgcgct acctggtacg tttcacgctg gtggatgtgg caacaggtga gtgggctacc
      661 tggtcgccgg tgaattatga atataaagtg cttccaccat tgcccgacaa gaatgaagcc
      721 agtacgactg atatgacaga gcagcaaatc atgcaactta agcaaaagac ctataaagcg
      781 atggtaaa
//
LOCUS       DAZGLJ010000595          783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.608, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000595 DAZGLJ010000000
VERSION     DAZGLJ010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.608"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..321
                     /locus_tag="ACHK49_005408"
     CDS             <1..321
                     /locus_tag="ACHK49_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO9265832.1"
                     /translation="REFGECKGKKHYHLMLLVNRDTWCRAGDYRAPGSLAGMIKQAWC
                     SALGVDVGCHATLVHFPAWPAVWLARNDDTGFQQVLERADYLAKEHTKVHCTGERNFG
                     CSRG"
     gene            487..>783
                     /locus_tag="ACHK49_005409"
     CDS             487..>783
                     /locus_tag="ACHK49_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO9265833.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHC"
BASE COUNT          173 a          215 c          225 g          170 t
ORIGIN      
        1 cgggagtttg gggagtgcaa aggtaaaaaa cactatcacc tgatgctgct ggtcaaccgg
       61 gatacctggt gtcgtgccgg tgattaccgc gctccgggat cactggccgg gatgattaaa
      121 caggcgtggt gcagtgccct gggagtggat gtcgggtgcc atgccacgct ggtgcatttt
      181 ccggcctggc cggcggtgtg gctggcgcgt aatgatgaca ccggctttca gcaagtgctg
      241 gaacgtgctg actatctggc gaaggagcat accaaagttc actgcaccgg tgagcgcaac
      301 tttggctgca gtcgtggctg agccagacag acgagttatc tgacttcagt gtgatgacgt
      361 gttcacggcc accacactga ttttccctta tgagactgaa ctgaaaccgc cattcacacc
      421 ggaccacgct gcccgtaccg ggacggctgt tgcctgcgtg tcgtctgacg gtaaggaaca
      481 tatactatgt acgcaaaatc ctttctcgct cttgatggca acggacgtct gacgggcgcc
      541 cgtacagcac aggccgcacc ttatgctcac tacacctgcc acttgtgtgg cagtgcactc
      601 agataccatc cgcaatacga cactgaactt ccctggtttg aacacactga cgacaggctg
      661 acagagcacg gtcaacagtg cccttatgtc aggccggagc gcagagaaat acagttgatt
      721 aaacgtctgc agcaattcgt accggatgcc ttacccgtgg tgcgtaaagc cagctggcac
      781 tgc
//
LOCUS       DAZGLJ010000596          776 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000596 DAZGLJ010000000
VERSION     DAZGLJ010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.609"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..111)
                     /gene="ariR"
                     /locus_tag="ACHK49_005410"
                     /gene_synonym="ymgB"
     CDS             complement(<1..111)
                     /gene="ariR"
                     /locus_tag="ACHK49_005410"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO9265834.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQA"
     gene            complement(140..412)
                     /gene="ymgA"
                     /locus_tag="ACHK49_005411"
     CDS             complement(140..412)
                     /gene="ymgA"
                     /locus_tag="ACHK49_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO9265835.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(455..691)
                     /gene="ycgZ"
                     /locus_tag="ACHK49_005412"
     CDS             complement(455..691)
                     /gene="ycgZ"
                     /locus_tag="ACHK49_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO9265836.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
BASE COUNT          223 a          166 c          129 g          258 t
ORIGIN      
        1 agcctgccct aacactgctg actcttcttc taacaaatta cccgaactgc gaaagtaact
       61 tgctaacgct ttatcagtta ttgcattatg aattgtagta tcttcaagca tggtaacctc
      121 tcatcttact tatgaaattt taatgtattc tgtttatttt cttaccattg aacatacttt
      181 tattgtcagc tgtcgctgtt cttcggtttg gccctcctga atcactattg ctaaaatcac
      241 gaattatgct gtcaattaaa tcgcgtcgga aagcatcttc ctcagccgat ttcattacga
      301 gtaattgctt aataagagtc tgcaatgaaa aatttatctt catgcgcagt atctggatca
      361 ccgcctggcc agtaatttca tattttatac gttcattatc agatgtcttc ataacgactt
      421 catagctaac ctgttaaata atcaggctgt tgcattattc aaaaagcaac ccaattagtg
      481 cattataatg tttctgttcc tcttccccag tcgcgtgttc cagtcggcaa agaagtttgg
      541 cacaaagcga ctttcgactc agattacgtc catctttcaa aatctcagtc acaatttccc
      601 cgagagtttc ctgctgagta ggcaagttag cttttgcaaa gtaacgagtg attgctcccg
      661 cagaatctaa agtcactgaa ttttgatgca tgctacgcct ctgttaaaaa tgttaatcaa
      721 tgttaagtga ggtaacaata gcaaacttgt acgaaatatg tgtacagaat gataaa
//
LOCUS       DAZGLJ010000597          769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000597 DAZGLJ010000000
VERSION     DAZGLJ010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.610"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..750
                     /locus_tag="ACHK49_005413"
     CDS             <1..750
                     /locus_tag="ACHK49_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFO9265837.1"
                     /translation="TEFSYLALPENYNRLFLPNSKNQTNNRFKTLNSKAIGRLLAAGG
                     VYNGNIEGFRDTAEKLGGDAIKGYDQILNEKTAGIAIATASILLTKRSNVDTYTEINS
                     YLGKLRGQQKLLDGIDIIEIIYIKRPSKDLANLRKEFNKTVRKNFLIKLAKTSEASGR
                     FNAEDLLRMRKGNVPLNYNVHHKLSLDDGGTNDFENLVLIENEPYHKVFTNMQSRIAK
                     GILVGESKITPWAIPSGSIYPPMKNIMDHTK"
BASE COUNT          303 a          135 c          129 g          202 t
ORIGIN      
        1 accgagttca gctatttggc actccctgaa aattataacc gtttgttttt accaaattca
       61 aaaaaccaaa caaataatcg tttcaaaacg ttaaacagca aagctattgg caggctactt
      121 gctgctggtg gcgtatacaa tgggaacatt gaaggattca gagatactgc ggaaaaactg
      181 ggtggagatg caataaaagg ctatgatcaa atactaaatg aaaaaacagc gggcatagcg
      241 atagcaacag catctattct tttaacaaag cgttctaatg ttgatacata tacagaaata
      301 aatagttact taggcaaact tagaggtcaa caaaaacttc ttgatggtat agacataata
      361 gaaataatat acattaagag accttcaaaa gacttagcta acttacgaaa ggagtttaat
      421 aaaactgtaa gaaaaaattt tcttatcaaa cttgcaaaaa cctccgaagc atctggaaga
      481 ttcaacgccg aagacctttt aagaatgaga aagggcaatg ttcctctaaa ttataatgtt
      541 caccataaac tatctctaga tgatggtggt actaatgatt tcgaaaattt agtattaatc
      601 gaaaacgaac catatcataa agtttttact aacatgcaat cacgaatagc taagggaata
      661 ttagtaggtg aaagcaaaat cactccctgg gccattccat ctggctcaat ttatcctccc
      721 atgaaaaata ttatggacca cacaaaatga tgactaaaac ccaaataaa
//
LOCUS       DAZGLJ010000598          768 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000598 DAZGLJ010000000
VERSION     DAZGLJ010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.611"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..659
                     /locus_tag="ACHK49_005414"
     CDS             <1..659
                     /locus_tag="ACHK49_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO9265838.1"
                     /translation="LNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQ
                     DPIGLKGGWNLYTYPLSPVNSMDPLGLYEFKSKNIDDIGIFALAMCNGESINENKEYG
                     GLICKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYSDDKGNKVTKE
                     NDVYDSLNFSSKDLTNSYMNGMGKKEYSSYLGTPNNTYLKYNPKAKGNGVTIIRQGSN
                     "
     gene            661..>768
                     /locus_tag="ACHK49_005415"
     CDS             661..>768
                     /locus_tag="ACHK49_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFO9265839.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAA"
BASE COUNT          267 a          119 c          174 g          208 t
ORIGIN      
        1 tgctgaatga agagaacccg catcagctgc agcagcttat ccgcctgccg gggcagcagt
       61 atgatgagga gtccggcctg tattacaacc gccaccgcta ttatgacccg ctgcaggggc
      121 ggtatatcac tcaggatccg attgggctga aggggggatg gaacctgtat acatatccgc
      181 tgagcccggt gaatagcatg gatccattag gattatatga atttaaatca aaaaatatag
      241 atgatattgg aatatttgca ttggcaatgt gtaatggaga atcaattaac gagaataaag
      301 aatatggtgg actaatatgt aagaagcaag gtgaatattt ccccatgaat ccgataagtt
      361 caaatgataa tgatagtgta gacttgcgaa atataaaatg ccctgaaggt tcagagagag
      421 taggcgatta tcacactcac ggtttttact ctgacgataa aggaaataaa gtaacaaaag
      481 aaaatgatgt ttatgatagt ctaaattttt caagcaaaga tttaacgaat tcttatatga
      541 atggaatggg aaaaaaagaa tacagtagtt acttgggaac accaaataac acctatctaa
      601 aatataatcc caaagctaaa gggaatggag ttacaattat caggcaaggg agtaattaaa
      661 atgaaggcaa tacaatgttg ttatttcctt gctttagtct ccagtcattt aatggctgga
      721 gttatgggtg atgataatat atttttaagt aattcggatt cagctgct
//
LOCUS       DAZGLJ010000599          765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000599 DAZGLJ010000000
VERSION     DAZGLJ010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.612"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..181
                     /locus_tag="ACHK49_005416"
     CDS             <1..181
                     /locus_tag="ACHK49_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO9265840.1"
                     /translation="SDSELNKINNYVDEYYKQWGKQYVRFNVNLKNQDTNNSSFSYGD
                     NRFEKSQGSNWTFQE"
     gene            195..435
                     /locus_tag="ACHK49_005417"
                     /pseudo
     CDS             195..435
                     /locus_tag="ACHK49_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            455..765
                     /gene="sctI"
                     /locus_tag="ACHK49_005418"
                     /pseudo
     CDS             455..765
                     /gene="sctI"
                     /locus_tag="ACHK49_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
BASE COUNT          274 a          119 c          157 g          215 t
ORIGIN      
        1 aagtgacagt gaattaaata aaattaataa ctatgttgac gaatattata aacaatgggg
       61 taaacaatat gtaagattta atgtgaattt gaaaaatcag gacacaaata atagttcatt
      121 tagctacgga gataaccgat tcgagaagtc tcaaggtagc aactggacat ttcaggaata
      181 attgaggagg acttatggca gattggaatg gttatattat ggatatcagc aatcaatttg
      241 atcagggcgt tgatgatctg aaccagcaag ttgaaaaagc gttggaggat ttagccaacc
      301 aatccctccg acacgaaatt tcttgccgaa tatcaaagtg cattagctga gtatacatta
      361 tatcgaaatg cgcaatctaa cgttgtaaaa gcgtataaag atcttgattc agcaatcata
      421 caaaacttcc gctaaactag ataaggattt tattatgtta gtaagcaata tgccacctat
      481 tgatagagct gagcaatcaa ctgctcatga aatacagcaa gcgaaagtga ttgatttaaa
      541 tgaccgggtt ctcaatcttg acaacccgga tgataaaatg atctccgctt ttgccaacta
      601 tgctgtacaa actgaaaatt ggcagcaaaa tgcgttacaa gcattaaggt ctgataaaga
      661 ggggttaact cctgaaaaat tattagtctt gcaggatcat gtgcttaatt ataatgtcga
      721 ggtttcattg gtggaacatt agcaagaaag attgtcgctg cagtt
//
LOCUS       DAZGLJ010000600          761 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000600 DAZGLJ010000000
VERSION     DAZGLJ010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.613"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>761
                     /locus_tag="ACHK49_005419"
     CDS             <1..>761
                     /locus_tag="ACHK49_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO9265841.1"
                     /translation="KPLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPANHAA
                     QFGALVDKFRADLADMAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKT
                     EKNIHFVPFMTDENGVNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSAW
                     ARRGIISDRLATAILRHAGRVALNAGASSTVSEVRPSSPSGAEATGVTTLLSYLASES
                     EGSLKVQGWSASGGRAEVVSDAEGTGGKAVKLTKEA"
BASE COUNT          195 a          177 c          244 g          145 t
ORIGIN      
        1 aagccgctgt ataaggacct tatcggtcga acaaaagcag cactgaagaa gaatccgaaa
       61 aatgtgctgt ttgccgtggt gtggatgcag ggggaatttg attttggcgg tacgccggca
      121 aatcacgcag cacagtttgg tgcgctggtt gataaattcc gtgcagacct ggcggatatg
      181 gcaggtcagt gcgtcggtgg ctctgctggc ggtgttccct ggatatgtgg agatacgacg
      241 tatttctgga agcagaagaa cgaatccacg taccagacgg tgtacggcag ctataaaaac
      301 aaaacggaaa agaatatcca tttcgtaccg ttcatgaccg atgagaacgg ggtgaatgtg
      361 ccgacgaaca aaccggaaga agacccggac attccgggta tcggatatta cggttcgaaa
      421 tggcgtgaca gctcagccac ctggacgtca caggacaggg cgagccattt cagcgcctgg
      481 gcacgccgtg ggattatttc cgaccgtctg gcaacggcga ttttgcgcca tgcgggaaga
      541 gtggcgctaa acgcgggggc atcatcgaca gtatcagagg tgcgcccttc atcgccttcc
      601 ggtgcagaag ccacaggcgt cacaacactg ctctcttacc ttgccagcga gtcagaggga
      661 agcctgaaag tacagggatg gtcagccagt ggcggcaggg cagaagtggt cagcgatgcg
      721 gagggaaccg gaggtaaggc agtgaagctg accaaggagg c
//
LOCUS       DAZGLJ010000601          760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000601 DAZGLJ010000000
VERSION     DAZGLJ010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.614"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            58..>760
                     /locus_tag="ACHK49_005420"
     CDS             58..>760
                     /locus_tag="ACHK49_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YeeR"
                     /protein_id="HFO9265842.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLT"
BASE COUNT          169 a          196 c          191 g          204 t
ORIGIN      
        1 cttcaacgcc acattcgctg gcattaacaa taacatgata ttcatcacgg agtgactatg
       61 ttacagatag tcggcgcgct gatcctgctg atcgcaggat ttgccattct tcgccttttg
      121 ttcagagcat taatcagcac ggcttctgcg ctggcagggc tcatattgct gtgtctgttc
      181 ggcccggcct tactggctgg ctatatcacc gaacgcataa cccggttgtt ccatattcgc
      241 tggctggcag gcgtatttct gacgattgcc ggaatgatca tcagcttcat gtggggactt
      301 gatggtaaac atatcgcgct ggaggctcac acctttgact ctgtgaaatt tattctgacc
      361 accgctctcg ccggtggtct gctggctgtt cccctgcaga tcaaaaacat tcagcagaac
      421 gggatcacac cagaagatat cagcaaggaa attaacgggt attactgctg tttttatact
      481 gcctttttcc ttatggcgtg ttctgcatgc gcaccattga tcgcgttaca gtacgatatt
      541 tcaccgtcac tgatgtggtg gggcgggttg ttgtactggc tggctgcatt agtgacgctg
      601 ctatgggcgg ccagccagat ccaggcgctg aaaaaactga cctgtgccat cagccagaca
      661 ctggaagaac aaccggtgct caacagtaaa tcgtggctga ccagtttgca aaacgattac
      721 agccttcctg actcactgac ggagcgcatc tggctgacgc
//
LOCUS       DAZGLJ010000602          759 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000602 DAZGLJ010000000
VERSION     DAZGLJ010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.615"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..97
                     /gene="yjbL"
                     /locus_tag="ACHK49_005421"
     CDS             <1..97
                     /gene="yjbL"
                     /locus_tag="ACHK49_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFO9265843.1"
                     /translation="LRYRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            121..>759
                     /gene="yjbM"
                     /locus_tag="ACHK49_005422"
     CDS             121..>759
                     /gene="yjbM"
                     /locus_tag="ACHK49_005422"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO9265844.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNLGDNK
                     DAMPIEQCTFDVENKNDLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKE"
BASE COUNT          247 a          117 c          156 g          239 t
ORIGIN      
        1 gctgcgttat cgggatataa ttattgcaaa aatagatttg tttattcaac gaatcacgca
       61 taatttatgg catgcacgta aaaaaatgtg tttttaattg agcaaataaa tgatctcaaa
      121 atgtgggtca ataagtatat tgacgattgc actgatgagg atttaaacga tcgtgacttt
      181 attgcatcag ttgttgaccg ggctattttt catttcgcga ttaatagtat atgtaatctt
      241 ggggataata aagatgcaat gcccattgaa caatgtactt ttgatgtaga aaataagaat
      301 gaccttccct ccacggttca gctattttat gaggaatcta aggataatga acctttagcg
      361 aatatacatt ttcaagcaat aggttctggt tttttaacgt ttgttaatgc ctgccaggaa
      421 catgatgaca acagcttaaa attatttgct tcgctgttaa tttcactttc atattctagt
      481 gcctacgcag atttatcaga aacagtgtat attaatgaaa ataatgagag ctacctgaaa
      541 gctcagtttg aaaaattatc tcaacgtgat atgaagaagt acctgggaga gatgaagcgt
      601 ctggctgatg ggggagaaat gaattttgat ggctatctgg ataagatgtc acatctggtg
      661 aatgaaggaa cgctcgatcc tgatatttta agcaaaatgc gagatgctgc accacaatta
      721 attagcttcg cgaagtcgtt tgacccaacc tcaaaggaa
//
LOCUS       DAZGLJ010000603          757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000603 DAZGLJ010000000
VERSION     DAZGLJ010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.616"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            148..>757
                     /gene="dgcZ"
                     /locus_tag="ACHK49_005423"
     CDS             148..>757
                     /gene="dgcZ"
                     /locus_tag="ACHK49_005423"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO9265845.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDSFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVY"
BASE COUNT          200 a          147 c          174 g          236 t
ORIGIN      
        1 cactttttta tccgctgatt ttgttttaaa tcacaatgtt atctcagaga tgactcataa
       61 cttcggattt ctatgaaaat attgacgtaa ttttgctttt ctctcgttag aatagcgcgc
      121 acaaggaact gtgaaaaagg agtggcaatg atcaagaaaa caacggaaat tgatgccatc
      181 ttgttaaatc tcaataaggc tatcgatgcc cactaccagt ggctggtgag tatgtttcac
      241 agcgtggtcg cgagagatgc cagtaagcca gaaataacgg ataaccattc ttatggactg
      301 tgccagtttg gtcggtggat tgatcatctg gggccactcg ataacgatga attaccttac
      361 gttcggctaa tggattctgc ccatcaacat atgcataact gtggtcggga attaatgctg
      421 gctattgttg aaaatcactg gcaggacgcg catttcgact cctttcagga ggggttgctt
      481 tcttttactg cggcattaac cgattacaaa atttatttgc tgacgatccg tagcaatatg
      541 gatgttttga cgggattgcc ggggcgtcgg gttcttgatg aatcctttga tcatcagtta
      601 cgcaacactg agcctctgaa tctttattta atgttgttgg atattgaccg atttaaattg
      661 gttaatgata cctacgggca tttaatcggc gatgtagtat tacgcaccct ggcaacttac
      721 ttagccagtt ggacgcgtga ttacgaaacg gtttatc
//
LOCUS       DAZGLJ010000604          756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000604 DAZGLJ010000000
VERSION     DAZGLJ010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.617"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            61..495
                     /locus_tag="ACHK49_005424"
     CDS             61..495
                     /locus_tag="ACHK49_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO9265846.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
BASE COUNT          169 a          168 c          235 g          184 t
ORIGIN      
        1 gtactgcaca cactgccaga ccggaggatt cagtattccc cggacaactc aggaggaaat
       61 atgcgaattc tgaactgtta tatggcgaat gacagcaaag gccattttgt tacggcgaaa
      121 gaagctgcga agcacaaccg acaggacgtt ttgtgctgtg tgtcctgtgg atgcccgtta
      181 acacttcagc ggggcaatga cggacaacca ccgtggtttg aacatgacca gatgactgtc
      241 gctgaaaaaa tcctgctgcg atgtacctgg cttgacccgg cggagaaaga ggcccgtcgt
      301 ttgcatctgc agggcatgac ggttccggat tatacggtga aggtgagaaa gtggttttgt
      361 gtgatgtgtg acgaagatta tgagggggaa aagtgctgcc cacgctgcgg taccggggta
      421 tacagcaggg cgtgggggcg gcaggaggtg ccgtcggaag atgccagggc tgataatccg
      481 ttacagaggc tgtaatggtt gcctccggag cagtttgcgg tgatgccttt cccgggaatg
      541 gattgccagt gcggggactg tggagcttaa ttccggtgtt ggtggccttt ccgttgattt
      601 tatgccaaca gccccctgtg actgacaggc tgcctcgtca ttccattcgt atctccagta
      661 acagggggtt gtattgtatt ttattgtgcc ggttcaggtg tgcgtttccc ggcgcgtctg
      721 caccggctta accaaattca acagggataa aatagt
//
LOCUS       DAZGLJ010000605          755 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000605 DAZGLJ010000000
VERSION     DAZGLJ010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.618"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(59..604)
                     /locus_tag="ACHK49_005425"
     CDS             complement(59..604)
                     /locus_tag="ACHK49_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO9265847.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
BASE COUNT          175 a          212 c          179 g          189 t
ORIGIN      
        1 ctgtggctcc ggcctgaaca gtgagcgaag cccggcgcgg acaaaatgcc gcagcctgtt
       61 aacctgactg ttcgatatat tcactcagca accccggtat cagttcatcc agcgcggctg
      121 ctttgttcat ggctttgatg atatcccgtt tcaggaaatc aacatgtcgg ttttccagtt
      181 ccggaaaacg ccgctgcacc gacaggggga tcccgtcgag aatactggca atttcacctg
      241 cgatccgcga cagcacgaaa gtacagaatg cggtttccac cacttcagcg gagtctctgg
      301 catttttcag ctcctgtgcg tcggcctgcg cacgcgtaag tcgatggcgt tcgtactcaa
      361 tagtccctgg ctggagatct gtctcgctgg cctgccgcag ttcttcaact tcccggcgca
      421 gcttttcgtt ctcaatttca gcatcccttt cggcatacca ttttatgacg gcggcagagt
      481 cataaagcac ctcattaccc ttgccaccgc ctcgcagaac gggcattccc tgttcctgcc
      541 agttctgaat ggtacggata ctcgcaccga aaatgtcagc cagctgcttt ttgttgactt
      601 ccattgttca ttccacggac aaaaacagag aaaggaaacg acagaggcca aaaagctcgt
      661 tttcagcacc tgtcgtttcc tttcttttca gggggtgttt taaataaaaa cattaggtta
      721 cggcgaagaa gaacggaaac gccttaaacc ggaaa
//
LOCUS       DAZGLJ010000606          752 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000606 DAZGLJ010000000
VERSION     DAZGLJ010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.619"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            9..623
                     /gene="espF"
                     /locus_tag="ACHK49_005426"
                     /pseudo
     CDS             9..623
                     /gene="espF"
                     /locus_tag="ACHK49_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
BASE COUNT          197 a          186 c          168 g          201 t
ORIGIN      
        1 catgaattat gcttaatgga attagtcaag ctgtttctac actaggacgg catattacta
       61 gtgcggtaag ccgggtaagc tctgcggggt tatctggctt ttcagtgtcc cctcaggctg
      121 ttcgtctgaa tccgattagg gctcaatctc ctttttctcc cggtacgtcg aatatcaatg
      181 cgagaacgac ttttaatgtg agtagcccgg cgacttcatt tactccctct cgtccggccc
      241 caccgccgcc gacaagtgga caggcttccg gggcatcccg ccctttaccg cccattgctc
      301 aggcattaaa agatcattta gctgcctatg aaaagtcgaa aagtcttgat acttcaagcc
      361 ttaagccaag ccgcccggca ccgccgccgc cgacaagcgg gttggtatct gggtcatccc
      421 ggtctttacc gcccattgct caggcgctaa aagatcattt ggctgcctat gaaaaatcga
      481 aaagtcttga tacttcaggt cttaagccaa gccgcccggc accgccgcca ccgacaagtg
      541 gacaggcatc cgaggcatcc cggccattac cgcctattgc tcaggcatta aaagatcatt
      601 tagctgccta tgaactgtcg aaaaaggcat aaaaagtaga tttaaaatta ttaattactg
      661 ttgattgttt agagagttaa aagataaaaa ccgatattaa gttgcattgt gataaatgtg
      721 attggtaata tcggttttta ttgaaataaa ta
//
LOCUS       DAZGLJ010000607          746 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000607 DAZGLJ010000000
VERSION     DAZGLJ010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.620"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..245)
                     /locus_tag="ACHK49_005427"
     CDS             complement(<1..245)
                     /locus_tag="ACHK49_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9265848.1"
                     /translation="MQHIHGESLNESVKSEQSPRVVLWEIDLKAQGGERYFFCNELNE
                     KGEPVTWQGRKYQAYPIDGSGFEMNGKGSSARPSLTVS"
     gene            complement(245..586)
                     /locus_tag="ACHK49_005428"
     CDS             complement(245..586)
                     /locus_tag="ACHK49_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9265849.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNVRYS
                     DFSCTIEQVVN"
     gene            complement(579..>746)
                     /locus_tag="ACHK49_005429"
     CDS             complement(579..>746)
                     /locus_tag="ACHK49_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9265850.1"
                     /translation="SAGLVYSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVML
                     SMRRDGGMLSG"
BASE COUNT          157 a          267 c          161 g          161 t
ORIGIN      
        1 gacaccgtca gcgacgggcg ggcactgctg cccttcccgt tcatctcaaa gccactgccg
       61 tcaatcgggt atgcctggta cttccgcccc tgccaggtca ccggctcccc tttttcattc
      121 agctcattac agaaaaaata ccgctcacca ccctgggcct tcaggtcaat ttcccagagc
      181 accacccgcg gtgactgctc tgatttaacc gactcgttca ggctttcccc gtgaatatgc
      241 tgcatcagtt caccacctgc tcaatcgtac agctgaaatc actgtaccgg acgttatccg
      301 tgacgctcca ctcccggcac accaccctca ccgtccggtt atgtttcggc ggtcgccaca
      361 aaaaggcacg gtaaccacca tgccaggata aaaattcatc cagccagcgc cgggttgact
      421 catccgtcac ccggaacacc gcctgaaacg tcttcagttg aggattcagt cctgtggggc
      481 gacgctgttc ataaccgtca ccaaaccgta ccctcgccac cgacggtttc tcactcacct
      541 gcatcccttc acgcgggacc agatgcagcg tttttatctc agccactcag cattcctccg
      601 tcacgtcgca tggacaacat caccgcctgc acccgctggt caatcagctg cacaagactg
      661 cctgccgcct ccggccctat ctggccatta gtcccgtcat tctgaatggc gatatggtag
      721 accggggaat acaccaggcc cgcact
//
LOCUS       DAZGLJ010000608          745 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000608 DAZGLJ010000000
VERSION     DAZGLJ010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.621"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..459)
                     /locus_tag="ACHK49_005430"
     CDS             complement(<1..459)
                     /locus_tag="ACHK49_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO9265851.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDI"
     gene            complement(440..>745)
                     /locus_tag="ACHK49_005431"
     CDS             complement(440..>745)
                     /locus_tag="ACHK49_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9265852.1"
                     /translation="FSFQEARSAWGNCDWIGSGRMAIDGLKEVQEAVMLIEAGLSTYE
                     KECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFESGLRQSTEEEKSDSRAA"
BASE COUNT          143 a          245 c          204 g          153 t
ORIGIN      
        1 tatgtcacgc acacgggcga tgatgtcagc gcagtcaaat gcccccgcca ccatcccgcc
       61 gggcgtgtcc atatcgagca gaatgccgtc caccatcgga tcgctggcag cctgttgcag
      121 acgggcgata atgccgttgt aaccggtcat ccccgagtac ggctgcagcg cccgcgtccg
      181 gctgaccagc gtgccggaca ccggcagcac ggcgatgccg ttcatgacct gataactgcg
      241 ggcctgtcgt ggtccgtcat catcaccgga taatgccagc gtcgcgagtg cctcctgggc
      301 agtcaggctg tcgccggaca ccgcatccgt caggcggctg atcccaagct ggcctgcaag
      361 cgcacaaaag aaaacccgcg cataggcggg ttcaagcatc agcggctcat taaaagccat
      421 gctggcaata tgcgggagat tacgcagctc tgctgtcact cttctcctcc tctgttgatt
      481 gtcgcagtcc ggattcaaat gccgcagccg cccaggcggg cggtttaaga ccggctgcgc
      541 ggcgctccat cgtttcacgg acctgctggg caaaaatttc ctgatagtcg tcaccgcgtt
      601 ttgcgcactc tttctcgtag gtactcagtc cggcttctat cagcatcacc gcttcctgta
      661 cttctttcag accatcgatg gccatacgac cggagcctat ccagtcacag ttcccccagg
      721 cgctgcgggc ttcctgaaag ctgaa
//
LOCUS       DAZGLJ010000609          736 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000609 DAZGLJ010000000
VERSION     DAZGLJ010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.622"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..491
                     /locus_tag="ACHK49_005432"
     CDS             <1..491
                     /locus_tag="ACHK49_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265853.1"
                     /translation="TSPQLLGLKDNVYFCKIDSSQMLFPNQVGVGLTQIAPLIIAANI
                     VQDGLIAIEQPELHIHPALQLAVGDLFTQYPLDVKRPMFLVETHSEHILLRILKRIRQ
                     TTDNELPESNYPVKPDFISVIVFEDNNGSTVTRKIDITDDGDFKQKWPKGFFEERRGE
                     LF"
     gene            491..>736
                     /locus_tag="ACHK49_005433"
     CDS             491..>736
                     /locus_tag="ACHK49_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265854.1"
                     /translation="MFYECAISPEALFEIAIDRRNYRDFIKGFSTGGNFLYSELPKLK
                     RNKKQLLGLLNANHSELQKKRLEDLIIFLKNNKVSRVY"
BASE COUNT          249 a          123 c          135 g          229 t
ORIGIN      
        1 aaacctcccc tcaactttta ggtctgaaag ataatgttta cttctgtaaa atagattcaa
       61 gccaaatgtt atttccaaat caagtcggtg tcggattaac tcagatagca cctcttatta
      121 ttgcagccaa tatagttcag gatggattaa ttgctataga gcagcctgag ctacatattc
      181 atcccgcttt acaactcgct gtaggtgatc tgtttaccca atatcccctt gatgttaaaa
      241 gacctatgtt tttagtcgaa acacatagtg agcacatttt gcttcgaatt ttaaaaagaa
      301 ttagacaaac aacggacaat gaattacctg aatcaaatta cccagtaaaa cctgatttta
      361 tatcagtgat cgttttcgag gataataatg gttcgactgt tactaggaaa atagatatta
      421 ctgatgatgg tgacttcaaa caaaaatggc ccaaaggttt cttcgaagaa agaaggggag
      481 agctttttta atgttctacg aatgcgccat ttcaccagag gctttgtttg agatcgcaat
      541 tgatcggaga aattatcgtg acttcataaa aggattttct acgggcggta actttctcta
      601 tagtgaatta ccaaagctga agagaaataa aaaacaattg cttgggctat taaatgctaa
      661 tcatagtgaa cttcaaaaaa aacgattaga ggaccttatt atttttttga aaaataataa
      721 ggttagtagg gtttat
//
LOCUS       DAZGLJ010000610          727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000610 DAZGLJ010000000
VERSION     DAZGLJ010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.623"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(21..467)
                     /locus_tag="ACHK49_005434"
     CDS             complement(21..467)
                     /locus_tag="ACHK49_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO9265855.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(464..>727)
                     /locus_tag="ACHK49_005435"
     CDS             complement(464..>727)
                     /locus_tag="ACHK49_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HFO9265856.1"
                     /translation="EWVSCGKIHADIRGRSSRERMQSGAEMAQAEIRIWVRGQSGREI
                     TAASRLHVLSGPWRDRILNVVGLPVPDATGGRLEILCRLGGEK"
BASE COUNT          147 a          260 c          173 g          147 t
ORIGIN      
        1 aggatacaaa tccgcctccg tcatcgtctc agtacctcat caatggcccg gttcatccgc
       61 gcaatcgcca cctgagctgc ctgttcactg cgcacatcaa acgccgggcg cacaaacggg
      121 tgcggtggca tattcacggt ccccatttcc acaaaccgcc agtagaaagc attgcgcggg
      181 ttatccgcct tcatggtgtt atcgctgtta ccggtgtccg gattaacacc acggatatgg
      241 acaccggatt ccatcccgcc atcgcgggag cgccgggaaa ggaccaccac attgcggcgc
      301 agttttcccc tgcgtaccgg tgcccgtgac accacttctt ctttcagcac attcgcaccc
      361 gcacgggttg cctcacgcag cacccggtta ttttccgcac cactcagaag ctgcaaatcg
      421 cggctgatgt cctccagccc cgaaaaatcc agcagggttt cgatcatttt tcccctccca
      481 gccgacagag aatttccaga cgtccgccgg tcgcatccgg cacgggcagc ccgacaacgt
      541 tcaggatccg gtcacgccag ggaccactca gcacatgaag tcgtgacgct gccgtgattt
      601 cccgaccgga ctgaccgcgc acccagatgc ggatttccgc ctgcgccatt tccgcaccag
      661 actgcatccg ctcccggctg ctcctgccac ggatatccgc atgaattttc ccgcatgaca
      721 cccattc
//
LOCUS       DAZGLJ010000611          722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000611 DAZGLJ010000000
VERSION     DAZGLJ010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.624"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            79..>722
                     /gene="sctD"
                     /locus_tag="ACHK49_005436"
     CDS             79..>722
                     /gene="sctD"
                     /locus_tag="ACHK49_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     subunit SctD"
                     /protein_id="HFO9265857.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTIGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPVEFWIDGQPTPYEADQSLPVGKVIDIAGCCFIIGDIDHTLP
                     LSDVPERFSAKNRRKKRLILASVIGAAIALSGAIGSYVLLSPKAEPPAFTRADVYQQL
                     KENKLHAITLVWHGKNVALYGRCESTTDLTPFFNYLKEKNIFYYNNIICNNQIIS"
BASE COUNT          211 a          152 c          136 g          223 t
ORIGIN      
        1 tctactctat tcttagttga tgtaagttca ccatattttt tctcattgtt caaccaccag
       61 gatgaataaa atttaaaaat gttatcctca tataaaataa aacttctcaa tggtgcgatg
      121 cgtaatcggg aattacaact tccgatggga aacctaacga taggcacaga agataacgat
      181 attgtttatt ttccccttga acaaggcctc aatcagtttt tacttgatat acgtgaggaa
      241 ggtgttttcc ttttatctcc ggttgaattt tggattgatg gacagcccac cccctatgaa
      301 gcagatcagt cattacctgt cggaaaggtc atcgatatag cgggatgttg cttcatcatt
      361 ggtgatattg accacacctt gccattgtct gatgtgccag aaaggttctc tgccaaaaat
      421 cgacgtaaaa agcgtcttat cctggctagc gtgattggtg ctgcgattgc gttgtctggc
      481 gccatcggca gttacgtttt gctttcccca aaagctgaac ccccagcgtt taccagagct
      541 gatgtatacc agcagctaaa agaaaacaaa cttcatgcaa tcactctggt atggcatggg
      601 aaaaatgtcg cgctttatgg gcgttgcgaa tcaaccactg atttaactcc attttttaat
      661 tacctgaaag aaaaaaatat tttttattac aacaatatca tttgcaataa ccaaatcatc
      721 tc
//
LOCUS       DAZGLJ010000612          720 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000612 DAZGLJ010000000
VERSION     DAZGLJ010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.625"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            20..>720
                     /locus_tag="ACHK49_005437"
     CDS             20..>720
                     /locus_tag="ACHK49_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9265858.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYH"
BASE COUNT          238 a          123 c          161 g          198 t
ORIGIN      
        1 tttcgaggag atcattccag tgggacgtaa atgggccaat attgttgcta aaaaaacggc
       61 taaagacggt gcaacgtcta aaatttatgc aaaattcggt gtagaaatct atgctgctgc
      121 caaacaaggt gaacccgatc cagaattaaa cacatcttta aaattcgtta ttgaacgtgc
      181 aaagcaggca caagttccaa agcacgttat tgataaagca attgataaag ccaaaggcgg
      241 cggagatgaa acgttcgtac agggacgtta tgaaggcttt ggtcctaatg gctcaatgat
      301 tattgctgag acattgactt caaatgttaa ccgtacgatt gctaacgttc gcacaatttt
      361 caataaaaaa ggcggcaata tcggagcggc aggttctgtc agctatatgt ttgacaatac
      421 gggtgtgatt gtatttaaag gaacagaccc tgaccatatt tttgaaattt tgcttgaagc
      481 tgaagttgat gttcgtgatg taactgaaga agaaggtaat attgttattt atactgaacc
      541 tactgacctt cataaaggaa tcgcggcgct aaaagcagct ggaatcactg agttctcaac
      601 aacagaatta gaaatgattg ctcaatctga agttgagctt tccccagaag atttagaaat
      661 ctttgaaggg cttgttgatg cccttgaaga tgacgacgat gtacaaaaag tttatcataa
//
LOCUS       DAZGLJ010000613          709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000613 DAZGLJ010000000
VERSION     DAZGLJ010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.626"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>709
                     /locus_tag="ACHK49_005438"
     CDS             <1..>709
                     /locus_tag="ACHK49_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO9265859.1"
                     /translation="AGMSVTAGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     IHNASGLWADIVALGTRHSMKASTDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAK
                     HSVSELPVNWWVQPSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSG"
BASE COUNT          141 a          200 c          219 g          149 t
ORIGIN      
        1 ttgccggtat gtctgtgacc gcgggggtat atggtgctgc tggccattct tccgttgatg
       61 ttaaggatga tgacggctcc cgtgccggca cggtccggga tgatgccggc agcctgggcg
      121 gatacctgaa tctgatacac aacgcctccg gactgtgggc tgacattgtg gccctgggaa
      181 cccgccacag catgaaagcg tcaacggaca ataacgactt ccgcgcccgg ggttggggct
      241 ggctgggttc actggaaacc ggtctgccct tcagtatcac tgacaacctg atgctggagc
      301 cacaactgca gtatacctgg cagggacttt ccctggatga cggtaaggac aacgccggtt
      361 atgtgaagtt cgggcatggc agtgcacaac atgtgcgtgc cggtttccgt ctgggcagcc
      421 acaacgatat gacctttggc gaaggcacct catcccgtgc ccccctgcgt gacagtgcaa
      481 aacacagtgt gagtgaatta ccggtgaact ggtgggtaca gccttctgtt atccgcacct
      541 tcagctcccg gggagatatg cgtgtgggga cttccactgc aggcagcggg atgacgttct
      601 ctccctcaca gaatggcaca tcactggacc tgcaggccgg actggaagcc cgtgtccggg
      661 aaaatatcac cctgggcgtt caggccggtt atgcccacag cgtcagcgg
//
LOCUS       DAZGLJ010000614          703 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000614 DAZGLJ010000000
VERSION     DAZGLJ010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.627"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..703
                     /locus_tag="ACHK49_005439"
                     /pseudo
     CDS             1..703
                     /locus_tag="ACHK49_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
BASE COUNT          153 a          183 c          214 g          153 t
ORIGIN      
        1 tccaatgcca tggcttacgg tgaagggctt acggtgaagg gcttccgctg ccggattcat
       61 acgatgctcc ggatccgcgc attaaacagc tggcgcgccg cagtacagtg acgccgggcg
      121 gggctgcctg cagatataac gatattattc cggctgacca ctgtctgcat gatgtgcagg
      181 atatgagtac gctgaatcat ccgagggctg acctgagcaa agggcagtac ggctgtgtcg
      241 gccagggttt acatattgcc aaaaaactgc tcccgtatat cccgaataac gcggggatcc
      301 tgctggtacc atgctgtcgt ggtggttcgg catttaccca gggcgcggag gggacattca
      361 gcgagtccac gggggccagt caggattcgg cacgctgggg ggtgggcaag ccgttatatc
      421 aggatctgat ttcccgcaca aaagcggcat tgcagaaaaa tcccaaaaac gttctgctgg
      481 ccgtctgctg gatgcagggt gagtttgaca tgagcgccgc cacccacgta cagcaacctg
      541 cgctgtttac agccatgctg acacagtttc gtgctgacct ctccgtgttt aacgcgcagt
      601 gccatggtgg cagtgctgca gatgtgccgt gggtttgtgg tgacacgacg tattactgga
      661 aaaatacata cgctacccag tacgacaccg tgtacggcgg gta
//
LOCUS       DAZGLJ010000615          699 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000615 DAZGLJ010000000
VERSION     DAZGLJ010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.628"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>699
                     /gene="espL4"
                     /locus_tag="ACHK49_005440"
     CDS             <1..>699
                     /gene="espL4"
                     /locus_tag="ACHK49_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFO9265860.1"
                     /translation="IVCRHIAAQYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQ
                     TLALISSRPEGRCVAACGDFGLVMKAYFDKMESNGISVMAAILLVDNHALTVRLRIKN
                     TTEGCTHYVVSVYDPNVTNDKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHIINQ
                     SVAIIPALPKEQLLMLKGSVDEITPPLSPATMNLLMAIGQNHQLTQLMIQLQKMPELH
                     RTEMLTAYNSGHMNV"
BASE COUNT          234 a          132 c          136 g          197 t
ORIGIN      
        1 atagtatgcc gacacattgc tgcacaatat attaatgata tttatcagaa tgttgattac
       61 aaaccccatc aagatgatta ctcttcagct gaaaaatttc tcactcactt caacaaaaaa
      121 tgcaaaaacc agactttggc gttgatttcc agccgtcctg aggggcgttg tgttgctgcc
      181 tgtggtgatt tcgggctagt tatgaaggca tatttcgaca agatggaatc aaatggcatc
      241 agtgttatgg cagccatatt actggtggat aaccatgctt tgacggtccg gctaagaata
      301 aagaacacaa ctgaaggatg tacccattac gtggtttcgg tttatgatcc taatgtcact
      361 aacgataaaa taagaattat gagcgaaagc aaagaggata ttaaacacta ttctctgatg
      421 gattttatga atgtagatta tagcctcctg aaatggtcaa atgatcatat tattaaccaa
      481 tctgttgcaa taattccagc acttccgaaa gaacagctat tgatgttaaa aggatctgtg
      541 gatgaaataa cccctccatt atcaccagca acgatgaatt tgctaatggc aattggtcag
      601 aatcaccaac ttacgcaact gatgattcag ctccagaaaa tgccagaact acatagaaca
      661 gaaatgttga ctgcctataa tagtggacat atgaacgtt
//
LOCUS       DAZGLJ010000616          691 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000616 DAZGLJ010000000
VERSION     DAZGLJ010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.629"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..211)
                     /locus_tag="ACHK49_005441"
     CDS             complement(<1..211)
                     /locus_tag="ACHK49_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265861.1"
                     /translation="MSTGLRFTLEVDGLPPNAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQR"
BASE COUNT          228 a          136 c          151 g          176 t
ORIGIN      
        1 ggcgctgtac ttcatcgccc tgccatatcg tcaggtaggc cattttgtcc agcacctgcg
       61 caaattcaag ggagagaaac tgctggctga ccagggagag atcgagggaa aagagcgaag
      121 agagtgactg gttcagatga aaggaaacca ccgcaaaagc attcggcggc aggccgtcca
      181 cttccagtgt gaaacgtaat ccggttgaca tagcaacctc cgtgaaaatg aataataatc
      241 cgcacatatt gcatgtacaa catgtcgggc ggatgagata agcgagtcga catctggcgt
      301 taccggggaa tatcagaaaa atgtcatggt tatgtgatga tttagtctgg gattttactt
      361 cagagaaacg ggtgtctgtt tagtcaacaa aataatcaca aacgcaacgg atgggttctc
      421 gacgttattc acgacaggct cgtacattac agcgagtaat aaaaaacctg atatgcaaag
      481 tcaaaccaat attcataatg tctttttatt cacaacaata aataattcaa taacaatcat
      541 tgaaatgaat gatggtttgt ataaggaata tacgctaccg ccccaccctg ataacatgat
      601 ctgcacattg cacatcttaa cgttgttaaa tactggctca atagtggata ttttaaatta
      661 ggaatttatc aaattcactt gataaataga t
//
LOCUS       DAZGLJ010000617          682 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.630, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000617 DAZGLJ010000000
VERSION     DAZGLJ010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.630"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..303
                     /locus_tag="ACHK49_005442"
     CDS             <1..303
                     /locus_tag="ACHK49_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO9265862.1"
                     /translation="AVHFPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRN
                     EALDCFVYALAALRISISRWQLDLSALLASLQEEDGAATNKKTLADYARALSGEDE"
     gene            300..506
                     /locus_tag="ACHK49_005443"
     CDS             300..506
                     /locus_tag="ACHK49_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO9265863.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            503..>682
                     /locus_tag="ACHK49_005444"
     CDS             503..>682
                     /locus_tag="ACHK49_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9265864.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARA"
BASE COUNT          172 a          173 c          210 g          127 t
ORIGIN      
        1 gccgttcact tcccgaataa cccggatatt tttgatctga ccgaagcgca gcagctgaca
       61 gctgaagagc aggtcgaaaa atgggtggat ggcaggaaaa aaatactgtg ggacagcaaa
      121 aaacgacgca atgaggctct cgactgcttc gtttatgcgc tggcggcgct gcgcatcagt
      181 atttcccgct ggcagctgga tctcagtgcg ctgctggcga gcctgcagga agaggatggt
      241 gcagcaacca acaagaaaac actggcagat tacgcccgtg ccttatccgg agaggatgaa
      301 tgacgcgaca ggaagaactt gccgctgccc gtgcggcact gcatgacctg atgacaggta
      361 aacgggtggc aacagtacag aaagacggac gaagggtgga gtttacggcc acttccgtgt
      421 ctgacctgaa aaaatatatt gcagagctgg aagtgcagac cggcatgaca cagcgacgca
      481 ggggacctgc aggattttat gtatgaaaac gcccaccatt cccacccttc tggggccgga
      541 cggcatgaca tcgctgcgcg aatatgctgg ttatcacggc ggtggcagcg gatttggtgg
      601 gcagttgcgg gcgtggaacc caccgagtga aagtgtggat gcagccctgc tgcccaactt
      661 tacccgtggc aatgcccgcg ca
//
LOCUS       DAZGLJ010000618          675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000618 DAZGLJ010000000
VERSION     DAZGLJ010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.631"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..542
                     /locus_tag="ACHK49_005445"
     CDS             3..542
                     /locus_tag="ACHK49_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001450376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system domain-containing
                     protein"
                     /protein_id="HFO9265865.1"
                     /translation="MVHDEWLGDLTGVNQVSFRHLRESSSTRNQLNKILRQEIQDKIS
                     GVELSDINKEGFLYQSIGKIRLLALSSALFEIQCPDYIFSRLYRETLIREIGYQNVKQ
                     LSFYWQGGQCKPEYGEERFCSELIKYGAGNLEWLFSDNPLWTIVKYLLPKSGEIKPTH
                     INDLFLNRLNKILLPYETL"
     gene            529..>675
                     /locus_tag="ACHK49_005446"
     CDS             529..>675
                     /locus_tag="ACHK49_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFO9265866.1"
                     /translation="MKLYEYKNIKIDLVLTEDIIPEDKLLEIIQSDDIVKLARKKTCE
                     HLLRA"
BASE COUNT          242 a           89 c          131 g          213 t
ORIGIN      
        1 tgatggtcca tgatgaatgg ctaggtgatt taaccggtgt caatcaggtt agttttcggc
       61 atttaagaga aagtagttct actcgtaatc aactcaacaa gatattgcgt caggaaatac
      121 aagataaaat ttctggagtg gaattatctg atataaataa ggaaggtttt ttatatcagt
      181 caattgggaa aataagattg cttgctctaa gtagtgcatt atttgaaatt caatgccctg
      241 actatatttt ttcacgatta tatcgagaaa ctctgatcag agagatagga tatcaaaatg
      301 tgaagcagtt atcattctat tggcagggag ggcaatgtaa accagaatat ggcgaagaac
      361 ggttttgttc tgaactgata aaatacggtg caggaaatct tgaatggcta ttttctgata
      421 atcccttatg gactatagtc aaatatcttt taccgaaaag tggagaaata aaaccaaccc
      481 atattaatga tttatttctt aacagactta ataaaatttt attgccttat gaaactttat
      541 gaatataaaa atataaaaat tgatttagtg cttacggaag atatcatacc agaagataag
      601 ttactggaaa ttattcaatc tgatgatata gtcaagttgg ctcgaaaaaa aacatgtgaa
      661 catttactga gggcg
//
LOCUS       DAZGLJ010000619          674 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000619 DAZGLJ010000000
VERSION     DAZGLJ010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.632"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..252
                     /locus_tag="ACHK49_005447"
     CDS             <1..252
                     /locus_tag="ACHK49_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO9265867.1"
                     /translation="KLGTFGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQS
                     PVSAFAADKLHVTDITRNATYPVLIDSIAVEVNSTDTAA"
     gene            388..567
                     /locus_tag="ACHK49_005448"
     CDS             388..567
                     /locus_tag="ACHK49_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO9265868.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDVWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            494..>674
                     /locus_tag="ACHK49_005449"
     CDS             494..>674
                     /locus_tag="ACHK49_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO9265869.1"
                     /translation="MTKGPLRKSAPVSQPEANPKSRSFSGNFLNHIFRRENPMSEITS
                     LVTAEAVKEVLRSEEV"
BASE COUNT          189 a          154 c          174 g          157 t
ORIGIN      
        1 aaactgggaa cctttggcgc atttgataac gaatggcata cgctggcttt ccgctttgcc
       61 gggaataaca gccttcaggt gacgccggtt attgatggtc aggatggcac accgttcacg
      121 ctgacgcagt caccggtcag tgcatttgcg gcggataaac tgcatgtgac agacattacc
      181 aggaatgcga cttacccggt actgatagac agcattgcgg tggaagtgaa cagcacagac
      241 actgcggcat gataaaaaaa ccgccatcga caggaatgga cgctggcggt ggtgatacct
      301 atggagaaaa aataaaggaa cgatactttc gtactctggt tttttaatga aaacagttct
      361 tattgtcaac aataacggaa agaaattatg acatttctga accagttaat gctgtacttc
      421 tgtacggtgg tctgtgtgct gtatctcctt tcgggtggat accgggccat gcgtgacgtc
      481 tggcgcagac agattgacaa aagggccgct gagaaaatca gcgccagtca gtcagccgga
      541 agcaaacccg aagagccgct catttagcgg caactttctt aatcacatct ttcgacgaga
      601 aaatcccatg tcagaaatta catccctggt cactgctgaa gcagtgaagg aagtcctgcg
      661 ctctgaagaa gtcc
//
LOCUS       DAZGLJ010000620          669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000620 DAZGLJ010000000
VERSION     DAZGLJ010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.633"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            111..>669
                     /locus_tag="ACHK49_005450"
     CDS             111..>669
                     /locus_tag="ACHK49_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HFO9265870.1"
                     /translation="MNTNIGMRYIDVVANGNAGEFYFAYWISSNFIWPCRILDIDMGL
                     DAQVEVYDDQNHSTGMFIGVQVKTTATTLEENPNVSVPMKNIAYWESINDPVVIIRTC
                     LNNNSKEPSLYWKHLPKSELRKYLNRAEEKGYETVTIMFEEDKNSLLKTDKSAWLKIF
                     LSDYDKEIIESVVGIKSKLDKLGKYL"
BASE COUNT          235 a           84 c          152 g          198 t
ORIGIN      
        1 gggttgtagg ttgtttagtt gaagtttaaa agcagcgctt tagcagttga ttgcgttaaa
       61 ccattgtaat atacaaaggg gaaatagtat ttagccgtgg gggagtatta atgaatacaa
      121 atatcggtat gagatatata gacgtagtgg caaatggtaa tgccggagag ttttattttg
      181 catactggat atcaagtaat ttcatctggc cttgccgtat tcttgatatt gatatggggt
      241 tagatgctca agttgaagtt tatgatgacc aaaatcattc aactggaatg tttattggtg
      301 tgcaggtgaa aacaaccgcg actacgctag aagaaaaccc taatgtttct gtacccatga
      361 aaaatatagc ttattgggaa agcataaatg atccagttgt tattattcgg acctgtctta
      421 ataacaattc aaaagagcct agcttgtact ggaagcatct tccgaaaagt gaattaagaa
      481 aatatttaaa tagagccgag gaaaaaggtt atgaaacagt aaccataatg ttcgaggagg
      541 ataagaactc attgctgaaa accgataagt cagcatggct aaagatattt ttgagcgatt
      601 atgataagga aatcattgaa agtgtagtag ggattaaaag taaactagat aagttaggta
      661 aatacttaa
//
LOCUS       DAZGLJ010000621          656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000621 DAZGLJ010000000
VERSION     DAZGLJ010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.634"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            111..290
                     /locus_tag="ACHK49_005451"
     CDS             111..290
                     /locus_tag="ACHK49_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO9265871.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAVRDCWRRQIDKRAAEKI
                     SAGQSAGSKPEEPLI"
     gene            331..576
                     /locus_tag="ACHK49_005452"
     CDS             331..576
                     /locus_tag="ACHK49_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO9265872.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
BASE COUNT          165 a          145 c          194 g          152 t
ORIGIN      
        1 tggacgctgg cggtggtgat acctatggag aaaaaataaa ggaacgatac tttcgtactc
       61 tggtttttaa tgaaaacagt tcttattgtc aacaataacg gaaagaaatt atgacatttc
      121 tgaaccagtt aatgctgtac ttctgtacgg tggtctgtgt gctgtatctc ctttcgggtg
      181 ggtacagggc agtgcgcgat tgctggcgca ggcagattga caaaagggcc gctgagaaaa
      241 tcagcgccgg tcagtcagcc ggaagcaaac ccgaagagcc gctcatttag cggcaacttt
      301 cttaatcaca cctttcgacg agaaaatccc atgtcagaaa ttacatccct ggtcactgct
      361 gaagcagtga aggaagtcct gcgctctgaa gaagtccgga gcgcactgaa acagaaactt
      421 cgccataacc tggaagcgcg tcttgatgca gaagtggatg ccattctgga tgaactgctg
      481 ggcgcaccgg cagctccgga gccggaaggc atcgcgggtg aggggagtgc ttcagatagc
      541 ggtgacccca caccggacag cgacatgatg atgtaagcat gcgtcaggga ccatcggtgt
      601 gtgccggtgg tcttttttat tgttgtgagc ttccggattg cgggaggcgg ggtatg
//
LOCUS       DAZGLJ010000622          655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000622 DAZGLJ010000000
VERSION     DAZGLJ010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.635"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>655
                     /locus_tag="ACHK49_005453"
     CDS             <1..>655
                     /locus_tag="ACHK49_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO9265873.1"
                     /translation="RARAKFHWQFLEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAE
                     YMQENRWDEESASFVPVETESDPINVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQ
                     ELLQEDMATFDGHIVEALMKMPEVNAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRI
                     WKKRREGERKETGKYTSVVDLARARANQQHTENSTGKISPVIAAIHREYKQTWKTLD"
BASE COUNT          189 a          157 c          165 g          144 t
ORIGIN      
        1 gtcgcgcccg cgcaaaattt cactggcaat tcctggaaca ttatccggct gctcaggact
       61 gcgcttataa atttcttgtt tgcgaggata aacccggtat tccccgccct gccctcgatt
      121 cctgggatgc tgaatatatg caggaaaacc gctgggatga ggagtctgct tcctttgtcc
      181 cggttgagac tgaatcagat ccgataaacg tcacttttga caagctggcc cctgaagtac
      241 agaacgctgt catggttaag ttcgacacat gtgaaaacat caccgttgat atggtgatta
      301 gcgcgcagga actgttgcag gaagacatgg caacattcga cggacatatc gttgaagcgt
      361 tgatgaaaat gccagaagtt aacgccatgt atccggagct taagctgcat gccatcgggt
      421 gggttaagca taaatgtaag ccaggtgcca aatggcccga aattcaggca gagatgcgca
      481 tctggaaaaa acgtcgcgaa ggtgaacgca aggaaaccgg aaaatacacg tctgttgttg
      541 atctcgcccg cgccagagcc aatcaacagc acactgaaaa ttcaacagga aaaatcagcc
      601 cggtcattgc tgccattcat cgcgaataca agcagacatg gaaaacactg gatga
//
LOCUS       DAZGLJ010000623          654 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000623 DAZGLJ010000000
VERSION     DAZGLJ010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.636"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..547
                     /gene="espX1"
                     /locus_tag="ACHK49_005454"
     CDS             <1..547
                     /gene="espX1"
                     /locus_tag="ACHK49_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO9265874.1"
                     /translation="QNIQYLTALNINIHKMLLSNITIEKSDLSYGYYFGCVLSNILCF
                     ESDLSNTIFSNGEINNLFIKKSNIFGASFTNTRIKNLLCEDIMPGRWTTQLVNKHLGY
                     RYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKELNKFMHDYDKTSSEM
                     RKYLKSINECMFLMKNIAHQN"
BASE COUNT          259 a          108 c           95 g          192 t
ORIGIN      
        1 gcaaaacatc cagtatttga ctgcccttaa tattaatata cataaaatgt tgctatcaaa
       61 tataacaata gaaaagagcg acctttcata tgggtattat tttggttgtg tattatccaa
      121 tatattatgt tttgaaagcg acttatcaaa caccatcttt tcaaatggag aaattaataa
      181 tctcttcatt aaaaaaagca acatatttgg agcatcattc actaatacca gaataaaaaa
      241 tttgctctgt gaagatatta tgcctggtcg atggaccact caacttgtca ataagcactt
      301 aggttacaga tacacaggag tattcaaaac cctggcaagt attgatgaca aaccgtcccg
      361 tttcgaaatt ttgataccat tggtacaaac tttagttcgc gacaatgtaa agctaaataa
      421 tgatgtgtat aaagaactta ataagtttat gcacgattac gacaaaacat catcagaaat
      481 gagaaaatac cttaaaagca tcaacgagtg tatgttcctt atgaaaaaca tagctcatca
      541 aaattaaaaa atctcaaaaa agaagtaatt tcagaatgaa attaactgtg atgattatta
      601 attcactaca gaggaataaa aaacaatctg aagcatgctc tgaaacatcc tact
//
LOCUS       DAZGLJ010000624          650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000624 DAZGLJ010000000
VERSION     DAZGLJ010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.637"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..>650
                     /locus_tag="ACHK49_005455"
     CDS             1..>650
                     /locus_tag="ACHK49_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265875.1"
                     /translation="MYNFITIMYDVFSCFGVLAKNQNSRDIRNIKNFSSHQHSLGDMF
                     DELINIIDKEQVLSKEQRKVIFRRYEDLYVKLMHYSVFTDKTHQIIKQKYFNDIVPMI
                     LALDIRNTYRPDNEMAFYYHIHSFLTQIPDNEDDIYHAARTYLRNYVKLCLSGYTPAN
                     AHFKDIFDGVYEFIRNIRKNSTPGKTKLIATINTCKETCKHLLYLSNEDKEKIISD"
BASE COUNT          242 a          110 c           99 g          199 t
ORIGIN      
        1 atgtataatt ttatcactat aatgtatgat gtcttttcat gttttggtgt tctggctaaa
       61 aaccagaata gccgtgacat ccgaaatatt aaaaattttt cctcacatca acattcactg
      121 ggcgacatgt ttgatgaatt aataaacatt attgataaag aacaagtatt gagtaaagaa
      181 caacgaaaag ttatatttag gagatatgaa gatctctatg ttaagctaat gcactattct
      241 gtttttacag acaaaacaca tcaaataata aaacaaaaat attttaatga cattgtacca
      301 atgattctcg cactcgacat caggaacaca tatcgcccgg ataatgagat ggcattttac
      361 tatcatattc attcttttct cactcagata ccggataatg aggatgatat atatcatgct
      421 gcaaggacat atctgcgaaa ttacgttaag ttatgtttat ccggatacac gccagcgaat
      481 gcgcatttca aagatatctt tgatggcgta tatgaattca ttcgtaatat tcgcaaaaac
      541 agtacaccag gaaaaacaaa acttatcgca actatcaaca catgcaaaga aacctgtaaa
      601 catctgcttt atttaagtaa tgaagacaag gaaaaaataa tttctgactt
//
LOCUS       DAZGLJ010000625          649 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000625 DAZGLJ010000000
VERSION     DAZGLJ010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.638"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            293..>649
                     /locus_tag="ACHK49_005456"
     CDS             293..>649
                     /locus_tag="ACHK49_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265876.1"
                     /translation="MNIQPIVTSGITTQNNRHHHAEQTSPTQIPQSELPNGCETGFVV
                     HIPEDMQRHAPECGETTALLSLIKDEGLLSGLDKYLAPHLEEGSAGKKALDMFGLFNV
                     SQMALEIPSTVPGISGK"
BASE COUNT          245 a          123 c          105 g          176 t
ORIGIN      
        1 ataagactaa tatcagtata aaatatttat aacacttaga gtaaaaatgt aatcagctta
       61 tacaaccaac aataatttaa actataaaaa caataacaga aacatttata atttatttta
      121 agtgttcaag cgataaactg gtaaatttaa caacttaatt ttgagatcta agtcacatca
      181 atcaagcatt caaacagcta tatattaaag ctgtccacat cggatatgtg acactaataa
      241 tatcaatgga ttgatattat taatggatat aaacatgcaa taaggattta tcatgaacat
      301 tcaaccgatc gtaacatccg gaatcaccac acaaaacaat cgacatcatc acgcagaaca
      361 aacgtcccct acacaaatac cgcaatccga attacctaat ggatgcgaaa cgggatttgt
      421 tgttcatatc ccagaggata tgcagcgaca tgcaccggaa tgcggtgaaa caacagctct
      481 actgagcttg ataaaagatg aaggtctgct ctctgggctg gataaatatc ttgcacctca
      541 tcttgaagaa ggctctgcag gaaaaaaagc attggatatg tttggtttat tcaatgtctc
      601 tcagatggca ttagaaatac ccagcaccgt tccgggtatc tctggtaaa
//
LOCUS       DAZGLJ010000626          643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000626 DAZGLJ010000000
VERSION     DAZGLJ010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.639"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>643)
                     /locus_tag="ACHK49_005457"
     CDS             complement(<1..>643)
                     /locus_tag="ACHK49_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9265877.1"
                     /translation="FVQATWDTSSSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQI
                     NDSGAALGYYVSEDGYPGWMPQKWTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYS
                     VMEQMKMLDTLQNTQLQSAIVKAMYAATIESELDTQSAMDFILGANSNEQRDKLAGWI
                     GEIAAYYAAAPVRLGGAKVPHLMPGDSLNLQTAQDTDNGYSVFEQSLLRYIAAG"
BASE COUNT          127 a          209 c          171 g          136 t
ORIGIN      
        1 ccggcagcga tataccgcag cagtgactgc tcaaacacgg agtagccgtt atccgtgtcc
       61 tgagccgtct gcaggttcag agagtcaccc ggcatcaggt gcggcacttt tgcgcctccc
      121 agccggaccg gcgcggcggc gtaatacgcg gcaatttcac caatccagcc cgccagcttg
      181 tcccgctgct cattactgtt cgcgccaaga ataaaatcca tcgctgactg cgtatccagc
      241 tcactttcaa tggtggcggc atacatcgcc ttcacaatgg cgctctgcag ctgcgtgttc
      301 tgcagcgtgt cgagcatctt catctgctcc atcacgctgt aaaacacatt tgcaccgcgg
      361 gtctgcccgt cctccacggg ttcaaagacg tgaatgaacg aggcgcgccc gccgggtaac
      421 tcacggggta tccatgtcca tttctgcggc atccagccag gatacccgtc ctcgctgacg
      481 taatatccca gcgccgcacc gctgtcatta atctgcacac cggcacggca gttccggctg
      541 tcgccggtat tgttcggatt gctgatgcgc ttcgggctga ccatccggaa ctgtgtccgg
      601 aaaagccgcg acgaactggt atcccaggtg gcctgaacga aca
//
LOCUS       DAZGLJ010000627          642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000627 DAZGLJ010000000
VERSION     DAZGLJ010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.640"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>642
                     /locus_tag="ACHK49_005458"
     CDS             <1..>642
                     /locus_tag="ACHK49_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspD"
                     /protein_id="HFO9265878.1"
                     /translation="DNKTLEFKKQLEEQQKAEEKAQKSKILGQVFGWLGVAVTAIAAI
                     FNPALWAVVAVSATAMALQTAVDVMGDKAPQALKTAAQVFGGVSIAASILTAGIGGVS
                     SLMSKVGDVANKVGSNIVKTVTKLADVFVENVVSKVAATANGFTTSARSIGTTVLNND
                     SAQYSILAQLSAYAVQNLTRQSENLGESAKVELDKAAAELRNQASYLQNASQLI"
BASE COUNT          186 a          126 c          167 g          163 t
ORIGIN      
        1 gataataaaa cgctggagtt taaaaagcag ctcgaagaac aacagaaagc cgaagagaaa
       61 gcacaaaaaa gtaaaattct tggtcaggtc tttggttggt tgggcgtcgc ggtaactgct
      121 attgcagcta tttttaaccc ggcactctgg gctgttgttg ccgttagcgc aacagcaatg
      181 gcactgcaaa ctgcagttga tgtaatgggc gataaggccc cgcaggcgtt aaagacagca
      241 gcgcaagtct ttggcggagt atctattgct gcaagtattc tgacagccgg cattggcggg
      301 gtgtcttcac ttatgtctaa agttggcgat gtcgctaaca aagttggttc aaacatcgtg
      361 aaaacagtta ctaaactggc cgatgtattt gttgagaacg ttgtttcaaa agttgcggca
      421 acggcaaatg gatttacgac ctctgcacgt tctattggga caactgtgct gaataatgat
      481 tctgcgcaat acagcatatt ggctcagtta tctgcttacg cggtacaaaa cttaactcga
      541 cagagtgaaa acctgggtga gagtgcgaag gttgagctgg ataaagctgc agccgagttg
      601 cgaaaccagg caagctattt acaaaatgct tctcagttaa ta
//
LOCUS       DAZGLJ010000628          640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000628 DAZGLJ010000000
VERSION     DAZGLJ010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.641"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(12..323)
                     /locus_tag="ACHK49_005459"
     CDS             complement(12..323)
                     /locus_tag="ACHK49_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO9265879.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     I"
     gene            complement(328..543)
                     /gene="essD"
                     /locus_tag="ACHK49_005460"
     CDS             complement(328..543)
                     /gene="essD"
                     /locus_tag="ACHK49_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO9265880.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
BASE COUNT          165 a          162 c          125 g          188 t
ORIGIN      
        1 gatttttgtg ttcatatata tttatgatga ggacgctcgt gcttattggc aggattttca
       61 atcttaaagg agtactgatg ctgcagataa gactcaactt tttctgacaa tttttctgct
      121 acttccagga agacttgccg gacgctcctt ctggctgctg cctcataaaa ctccagcgca
      181 gctccttcaa cacggtccat ggcgacatcc aggtcaaaaa tttcaccgtc aaagcgttcc
      241 ttgtcctgta aggctacagt taccgtaact ttattctcaa aattacggac tcctttcaca
      301 accagttcat agtcttgagt cattggatta ctctcctctc gcagccttac gcctgtcttc
      361 tttaatcttg aaataaagat ttgtcagata cgtcagcagg ccaaaaacca ggctacccag
      421 cacaccgatt gcagcccact gtgacggagt tactttatcg agtaactgta atgcccagaa
      481 accagcatta cccgccgatg tgccataggc aacacctgtt gttaacttat ccattgattt
      541 catatcctca ccccgatgta cacggatggt gcaatatgtt tgaaaaagat cggagtctac
      601 ggggtagttt tgacagcaca cgttgttctc aacggcgcta
//
LOCUS       DAZGLJ010000629          636 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000629 DAZGLJ010000000
VERSION     DAZGLJ010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.642"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            238..453
                     /gene="essD"
                     /locus_tag="ACHK49_005461"
     CDS             238..453
                     /gene="essD"
                     /locus_tag="ACHK49_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO9265881.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            453..>636
                     /gene="rrrD"
                     /locus_tag="ACHK49_005462"
     CDS             453..>636
                     /gene="rrrD"
                     /locus_tag="ACHK49_005462"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO9265882.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIM"
BASE COUNT          160 a          132 c          157 g          187 t
ORIGIN      
        1 gcaaatatca tttagtggtt tacgtacgta aaaaattggt tatgctgtta agagtggtta
       61 cttcgtcaca cagcttaaac ccgccgtcga gcgggttttt ccattttttg agtctcgata
      121 ttagctgata acccaatacc tgagttattc actgactccg agtctgttac gtttctgctt
      181 ttttgcgata cgttgtattc cctcaattta cacccgcttt gtctgcgagg tggggttatg
      241 aaatccatgg ataagttaac aacgggtgtc gcctatggca cctcagcagg tagtgccggt
      301 tactggtttt tacagctgct cgataaagtc acgccctcac agtgggcagc aataggtgtg
      361 ctgggtagcc tggtatttgg cctgctgacg tacctgacaa acctttattt caagattaaa
      421 gaagataagc gcaaggctgc gagaggtgaa taatgcctcc atcattacga aaagccgttg
      481 ctgctgctat tggtggcgga gcaattgcta tagcatcagt gttaatcact ggcccaagtg
      541 gtaacgatgg tctggaaggt gtcagctaca taccatacaa agatattgtt ggtgtatgga
      601 ctgtatgtca cgggcataca ggaaaagaca tcatgc
//
LOCUS       DAZGLJ010000630          634 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000630 DAZGLJ010000000
VERSION     DAZGLJ010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.643"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>634
                     /locus_tag="ACHK49_005463"
     CDS             <1..>634
                     /locus_tag="ACHK49_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO9265883.1"
                     /translation="ILNAAGRTSAFISGKAPEIKPSPGGNTPSGPSADTSVRTISLLP
                     AAGEAAAQGWSIKDGGIQLSDGVFKITKQSNKTWSLTHPVDDAITLLTQGGRLTCKFR
                     LSGALTNNQFGLGIYLYTDAPVPDGVAMTGTGNPFLMSYFTQTTDGRVNLMHHRKAGN
                     TKLGEFGDYGNDWQTLELVFTAGSATVTPKLNGVAGPAFQVIKDSLTLGLN"
BASE COUNT          146 a          172 c          188 g          128 t
ORIGIN      
        1 attctgaacg cagccgggcg cacctccgcc ttcatcagtg gtaaggcacc ggaaatcaaa
       61 ccctcgcccg gcggcaacac gccatcgggt ccgtctgcag atacgtccgt tcgcacaatc
      121 tccctgctgc cggcagccgg agaggctgct gcgcagggct ggagcattaa ggatggcgga
      181 attcagttgt cagatggtgt atttaagatc accaagcaga gcaataaaac ctggtccctg
      241 acgcatccgg tggatgacgc aattaccctg ctgacacagg gcggcagact gacctgtaag
      301 ttccgcctgt caggcgcact gaccaacaat cagttcgggc tggggattta tctgtatacg
      361 gatgctcccg ttcctgatgg tgtggcgatg acgggtaccg gtaatccgtt cctgatgtcg
      421 tacttcactc agaccactga cggcagagtg aatctgatgc atcacaggaa agccggaaac
      481 acgaagctgg gggagttcgg cgattacggt aacgactggc agacgctgga gctggtgttc
      541 accgccggca gtgccacggt tactccgaaa ctgaatggag tggctggccc ggcattccag
      601 gttataaaag acagtctgac actgggactg aatg
//
LOCUS       DAZGLJ010000631          633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000631 DAZGLJ010000000
VERSION     DAZGLJ010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.644"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..467)
                     /locus_tag="ACHK49_005464"
     CDS             complement(<1..467)
                     /locus_tag="ACHK49_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265884.1"
                     /translation="MPLYEWLINKRLRYQYLTLLAFSILALLALYLLYRNTPKVSVNF
                     FEFYHKNLRGYLFSGFISVGSFLLSLHTFVIINLRDKVFATQEYKEIYSIATGIPIDK
                     INDSVLYKPLDNLSSFINTSILCSITTAIAQFTIGLSTNLYACLFCVWLAILTV"
     gene            complement(454..>633)
                     /locus_tag="ACHK49_005465"
     CDS             complement(454..>633)
                     /locus_tag="ACHK49_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265885.1"
                     /translation="AGIEKTIDFMNCPVFFETYDFDIIADKVNGLTNDNYTTNPVFDM
                     IKEEMLNGTNKNAFI"
BASE COUNT          218 a           97 c          109 g          209 t
ORIGIN      
        1 accgttagta ttgcaagcca tacgcaaaat aagcatgcat ataaattagt tgaaagtcca
       61 atagtgaatt gtgcaattgc tgttgtgata gaacataata ttgatgtatt aataaatgag
      121 gataaattgt ctaaaggttt ataaagtaca ctatcattga ttttgtcaat aggtatacca
      181 gtggcaatgc tataaatttc cttatattcc tgagttgcaa aaaccttatc gcgtaaattt
      241 attatgacaa aggtatgcag gctcaataaa aatgagccca cggaaataaa accggagaag
      301 agatagcctc gtaagttttt atgataaaac tcaaaaaagt taacacttac tttaggtgtg
      361 tttctgtaca ataggtaaag tgcaagcaat gccaggatcg agaatgcaag cagtgtaagg
      421 tactgatacc tcaatctttt atttattagc cattcatata aaggcatttt tattcgtccc
      481 gttcagcatt tcttctttta tcatatcaaa aacagggttt gttgtataat tatcgtttgt
      541 caatccattg actttatcgg caattatatc gaaatcgtat gtttcaaaaa aaacagggca
      601 gttcataaaa tcaatggttt tttctattcc tgc
//
LOCUS       DAZGLJ010000632          629 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000632 DAZGLJ010000000
VERSION     DAZGLJ010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.645"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..626
                     /locus_tag="ACHK49_005466"
     CDS             <1..626
                     /locus_tag="ACHK49_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO9265886.1"
                     /translation="YNNRPHDELPVKPNGKHYSPAEFRKKRLAEEDTEIEWLSDVELR
                     DMFRPMVERPVRRCEIRWLNNIYYAPELRDEHGRKVLISYDIHDAERITVRRLDGSVI
                     CEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRAEEKIRDAEDEVVNVLEHKPQEPWLE
                     NIYRPVGNTVTVQQPVADDEPDEEYERNFQRGLQLLEAKLKENDPLA"
BASE COUNT          177 a          148 c          189 g          115 t
ORIGIN      
        1 ggtacaacaa ccggccgcac gatgaattac cggtgaagcc gaacggcaag cattacagcc
       61 cggcggagtt cagaaaaaaa cgcctggcgg aagaggacac ggaaattgaa tggctgtccg
      121 atgttgagct gcgggatatg ttccggccga tggtggaacg ccctgtaaga cgctgtgaaa
      181 tacgctggct gaataatatt tactacgcgc ccgagctgcg tgacgagcat ggccgtaagg
      241 tgcttatcag ctatgacatt catgatgccg aacgaattac cgtacgtcgc ctggatggca
      301 gcgtgatttg cgaggcggta tgggacggca ataaacgcga agccttcccg gtcagcgcgg
      361 aatactacaa acagcagcaa cgccttaaag gcatgcgtaa acgcgcagag gaaaaaatcc
      421 gtgatgccga ggatgaggtt gtcaacgtgc tggagcacaa gccgcaggag ccctggctgg
      481 aaaacatata ccgccctgtg ggtaatacgg tgaccgttca gcaaccggtc gctgacgatg
      541 aacctgatga agaatacgag cgtaatttcc agcggggatt gcagttgctg gaagcgaaat
      601 taaaagaaaa tgacccgctg gcctgaaat
//
LOCUS       DAZGLJ010000633          628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000633 DAZGLJ010000000
VERSION     DAZGLJ010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.646"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            61..465
                     /locus_tag="ACHK49_005467"
     CDS             61..465
                     /locus_tag="ACHK49_005467"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9265887.1"
                     /translation="MKSLTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGIND
                     NLLFKWRQYWREGKLRPPSTTENNVPELLPITLDAEDVVPTTSPRSQPVAAATPESLN
                     ISCEVTFRHGSLRLNGAISENILNLLIRELKR"
     gene            462..>628
                     /gene="tnpB"
                     /locus_tag="ACHK49_005468"
     CDS             462..>628
                     /gene="tnpB"
                     /locus_tag="ACHK49_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9265888.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVF
                     IFRGRNGSQVKL"
BASE COUNT          175 a          165 c          150 g          138 t
ORIGIN      
        1 tctggcagat cctgaaattc ctgagagaat agtggacacc aaatatggtg gacgctatcc
       61 atgaaatcat taaccgcagt gcgtaaaaaa agccctaatt atcccgttga gttcaaaatc
      121 aaaatggttg aactctcgca tcgaccagag atctccgtag cgcaactcgc tcgtgagcat
      181 gggatcaacg ataatttgct gttcaagtgg cgccagtact ggcgcgaagg aaaactacgt
      241 cctccttcaa caacagaaaa caacgtgcct gagctgctcc cgataacact tgatgccgaa
      301 gatgttgtcc ctacaacctc cccccggtca caacctgtag ctgctgcgac acctgaatca
      361 ctcaatatca gctgtgaagt gacgttccgg cacggatcac tccgtctgaa tggtgccatc
      421 agcgaaaata tcctgaacct gctgatacgg gagctcaaac gttgatccca ttaccatcag
      481 ggacaaagat ctggctggtc gctggcatca ccgatatgag aaacggcttc aacggcctgg
      541 cggcaaaggt gcagacgacg ctgaaagacg atccgatgtc aggtcacgtt tttatcttcc
      601 gtgggcgtaa tggcagtcag gtaaagct
//
LOCUS       DAZGLJ010000634          626 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000634 DAZGLJ010000000
VERSION     DAZGLJ010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.647"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>626
                     /locus_tag="ACHK49_005469"
     CDS             <1..>626
                     /locus_tag="ACHK49_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9265889.1"
                     /translation="GQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLDDKQQSALT
                     KTERELALSRLKGEEKERARLGYAADDLGFVGDSYQEARQRYISNALEAWRNNEANKP
                     KSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTAQGELKTLTEMQKQE
                     LLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERA"
BASE COUNT          177 a          142 c          207 g          100 t
ORIGIN      
        1 ggtcagcata cggaattcaa ccgtctgctg gggctgggga atgaactgct tcagcagcgt
       61 caggggctgg tgaatgtacc gttacggctg ccacaggcca ccctggatga taaacagcag
      121 agtgccctga caaaaacaga gcgtgagctg gccctgtccc gactgaaggg ggaggaaaaa
      181 gagcgtgccc ggctggggta tgcggcggat gaccttggtt ttgtggggga ttcgtatcag
      241 gaggcgagac agcgttatat cagtaatgcc ctggaagcct ggcgtaataa cgaggcgaac
      301 aaacctaaat cccggggtgg aaaatcagag acggaaaaag cggaagacag tttttcccgg
      361 ctactgaagc agcagaaaga gcaactggca ctggcgggtc agaatacaga gctggcgaag
      421 ctgaagtacc agactgcgca gggcgaactg aaaaccctga cggagatgca gaagcaggaa
      481 ctgctgcgta acgcggccct gattgaccag caaaaaatcc gggaacagtt gcgatcccgg
      541 gaagagaccc tgaagaatga taatgtggct gcgcgtgcat caaatgaagc cgaactgctg
      601 ggatacgggc agggagaacg agccag
//
LOCUS       DAZGLJ010000635          624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000635 DAZGLJ010000000
VERSION     DAZGLJ010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.648"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..547)
                     /locus_tag="ACHK49_005470"
     CDS             complement(<1..547)
                     /locus_tag="ACHK49_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO9265890.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGG"
     gene            complement(544..>624)
                     /locus_tag="ACHK49_005471"
     CDS             complement(544..>624)
                     /locus_tag="ACHK49_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9265891.1"
                     /translation="AWRHRHWHASAFTGICNDLAAASACT"
BASE COUNT          120 a          206 c          175 g          123 t
ORIGIN      
        1 caccaccttc atcacgggtg ctgatgtcct gggagattcg ccgggagcca accagcattt
       61 caccgtaagg caccggcatc gggttcccct gggcaatcat gttgtccagt gacgaaaaat
      121 acgtgttctg tttaccgtta tccgtacttt tgtactccgg cgtctttgcc ttcggggcca
      181 gcatctgggc cacaccgccc agtatcatgc tggcccccag tgaaaacagc atcgtggtgg
      241 cagaaaaacc gccggctgcc agggctgaac cccataacgc catcgttgcg ccggcggtga
      301 agaaagagcc cacgatggct gccgccccca gcacaatctg cagtccgcct ttccctgcac
      361 cggccagtcg cggcacaatg tggatgaccg ttccctcacc cagctgttcg tgaagacggg
      421 cgtacaccgc ctccggtgcc gtgtcctcac cgcgaatacg tatctggtac cagccttcgt
      481 tcatctgacg gcggaatccc ggcacctgta acgacagcgc ccggatggct tccgctgccg
      541 tgttcacgta caggctgagg cggcggccaa atcgttgcaa atccccgtga aggcagatgc
      601 gtgccagtgg cggtgacgcc aggc
//
LOCUS       DAZGLJ010000636          621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000636 DAZGLJ010000000
VERSION     DAZGLJ010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.649"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(6..140)
                     /locus_tag="ACHK49_005472"
     CDS             complement(6..140)
                     /locus_tag="ACHK49_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265892.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLIRAAKKQLEALCAMK
                     "
     gene            complement(216..527)
                     /locus_tag="ACHK49_005473"
     CDS             complement(216..527)
                     /locus_tag="ACHK49_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion protein"
                     /protein_id="HFO9265893.1"
                     /translation="MKLRVWHIPQVPMKPFIAEVASVEEGVRLMGALADYDAFQYDNN
                     IKPDYCNANGLEMWDESLTDEDLSEVGLTDRWVDWYSECQCYDDPRKYLESLKEETSA
                     A"
BASE COUNT          158 a          153 c          137 g          173 t
ORIGIN      
        1 aatggctatt tcattgcgca taacgcctcc agttgtttct ttgctgctct aattaattgt
       61 ttaactcggc gtgataattc agattcgtgc gggtagaaag cggacatgac gccgctaccc
      121 gcgagctgaa agtgcatcat gggtaactcc ttatatttga ttgcataacg aaaacgcctc
      181 gagtgaagcg ttattggtat gcggtaaagc cgcgcttagg cggctgatgt ttcttctttc
      241 aggctttcga gatatttacg tgggtcgtcg taacattggc attcgctgta ccaatccacc
      301 cagcgatcag taagccccac ctctgataaa tcttcatcgg taaggctctc atcccacatc
      361 tcaaggccgt tagcattgca gtaatcaggc ttgatgttgt tgtcatactg aaaggcgtca
      421 taatcagcca gtgcgcccat caggcgaaca ccctcttcaa cacttgccac ttctgcaatg
      481 aacggtttca taggtacttg cgggatatgc cagacacgta atttcatatt tcctccgtca
      541 aaaaaatcgc cctcacattg gagggcaaag aagatttcca ataatcagaa caagtcggct
      601 cctgtttagt tacgagcgac a
//
LOCUS       DAZGLJ010000637          618 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000637 DAZGLJ010000000
VERSION     DAZGLJ010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.650"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..173
                     /locus_tag="ACHK49_005474"
     CDS             <1..173
                     /locus_tag="ACHK49_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265894.1"
                     /translation="MSPGFVSSKKIDLNCLSEHALNRTVSHDNIFSSLLGLWNVNTSV
                     YNKEDDIISSCR"
     gene            complement(266..>618)
                     /locus_tag="ACHK49_005475"
     CDS             complement(266..>618)
                     /locus_tag="ACHK49_005475"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO9265895.1"
                     /translation="TTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALIISF
                     WLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAVLIT
                     ILVAIIIWGVLLLS"
BASE COUNT          202 a          121 c          108 g          187 t
ORIGIN      
        1 ggatgtctcc ggggttcgtt tcctctaaaa aaattgattt gaattgtttg tcagaacatg
       61 ctctaaacag aacagtaagt catgataata tattttcatc gttactgggg ttatggaatg
      121 taaatacttc agtttataat aaagaagatg atattatatc ctcctgcaga tagatactga
      181 catttttctg aggataagag gtgaatacct cttatcctta agtaaatatt aaccaggtaa
      241 ctgtgaataa tttagatgtt gggcgttatg aaagtaataa aactccccat ataatgatag
      301 caactaaaat cgtaatcaac actgcggctg aacccatatc ttttgctctg cctgataatt
      361 cgtgatgttc tgaaccaatt ctgtcaacaa ctgcttcaat tgcactgtta attatttcaa
      421 caataattac catcattgcc gaaccaatga gtaatactcg agtaacagag tcaacatcca
      481 gccaaaagga tattatcagt gctgacagaa ccagaatgca ctcctgacga aaggctgctt
      541 catagcgcca tgccgcacat aagcctttcc atgaataacc cgctgcttta ataattctgg
      601 taacaccagt ggtcgtat
//
LOCUS       DAZGLJ010000638          615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000638 DAZGLJ010000000
VERSION     DAZGLJ010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.651"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(29..571)
                     /gene="fimA"
                     /locus_tag="ACHK49_005476"
     CDS             complement(29..571)
                     /gene="fimA"
                     /locus_tag="ACHK49_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO9265896.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          181 a          132 c          132 g          170 t
ORIGIN      
        1 tatataattc aaaacaacgt ggttttgatt attcgtatct cataataaaa gtcgcgtcag
       61 catttgcttg ccctgctgat gcacttgtac cggtgccttt ataacgtgca gaaaaatgaa
      121 gaacgttggt cccggggatc aagttctggt tggttgagaa gctattccca tcaggtttga
      181 gaattgcgga tgtattatca agtatttcaa taccgacacc cgttgctgtt gttgtattgg
      241 tactggatgc aatggccaat aaattatcat tgattgcatc agcctgacca gaaaatgcaa
      301 cggcagcatt agcggcaaca acaggatcgc agtcgttcaa ctgaatggtg aatggaatta
      361 atgcagaggt attaccaaca gcattgaaaa tatcggtacg atattgaccg agtgtgacaa
      421 cctggtctgc cgagtcagta ttcactgaac aggcagcatt caccagttcg ccttcaaagt
      481 gaattgtacc gccatctaca acggcagcat aactacccgt agcggcgaat agtgcggcag
      541 ccagcgcaga cgaaataaat cttaatttca tatatattcc ttcaatctca tttatcgact
      601 ccacatccgt atata
//
LOCUS       DAZGLJ010000639          605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000639 DAZGLJ010000000
VERSION     DAZGLJ010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.652"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..532)
                     /locus_tag="ACHK49_005477"
     CDS             complement(<1..532)
                     /locus_tag="ACHK49_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265897.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGALPAGWRKRDDVAFIGDGPCFIARPDESCPEGPAIAAMIEA
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNMDSWWTPSLSRDALCVVFKVGAYGREIKG
                     GIPEECQEIKHSEYVAL"
BASE COUNT          164 a          162 c          129 g          150 t
ORIGIN      
        1 tcagcgccac atattcagaa tgtttaatct cctggcattc ttcaggaata ccccctttta
       61 tttccctgcc ataagcgcct actttaaaca ccacgcacag ggcatcacgg gagagcgacg
      121 gggtccacca ggagtccatg ttaaacatgg aggggattct cattaccccc agcttttcac
      181 agagccagac aaggaaatca ggacgctttc ttaactcgcg ttcagcggct tcaatcattg
      241 cggcaatcgc cggtccttca gggcatgact catcaggtct ggcaataaag cacggtccgt
      301 ccccgataaa agccacatca tcgcgcttac gccagcccgc aggcagagca ccagaaaaat
      361 aaaactcact gattacgtcc atggcaaacg gctttaagcg caatccggca gcgcctgtat
      421 ttttcagtaa tgcccgaaga atactgttgc gtagcggtaa aacagtctgt tgatatttgg
      481 tgaaatattt atttgtctgc acacggtcta atttgaaata aaaatatgcc atatatcctg
      541 ttccttattt tcagtttaca ggcgcaggca gtcccctgac gcgagcgcca tacttaaaac
      601 gatgt
//
LOCUS       DAZGLJ010000640          604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000640 DAZGLJ010000000
VERSION     DAZGLJ010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.653"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..425
                     /locus_tag="ACHK49_005478"
     CDS             <1..425
                     /locus_tag="ACHK49_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265898.1"
                     /translation="LSALPPSLRKKNFTDAYNAASSHIEYWIGAGVSFISMMILSRVY
                     DFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVRGKETETISERLI
                     READTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
     gene            525..604
                     /locus_tag="ACHK49_005479"
                     /pseudo
     CDS             525..604
                     /locus_tag="ACHK49_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT          192 a          109 c          104 g          199 t
ORIGIN      
        1 aactttcagc acttccacct tcattaagaa agaaaaattt tactgacgca tataatgcag
       61 catcttctca tatcgaatac tggataggtg ctggcgtctc atttatctcg atgatgattt
      121 tatccagagt atatgacttt cttttacccg cccaagatac tttcccgggt gatatcatca
      181 gaagtttatg tgttgtgtgt ccatccatat taatctggtt tcagttttcg gtatatgtca
      241 tgaggaaata ttacagacat attctggtgc gaggaaaaga aaccgaaacc atttcagaaa
      301 ggttgatacg tgaggctgat acacgagaat atgaactatg gcgtccggtc cgtcgctttt
      361 tctccattgt tttcctcatt gttctattag gttgcattca ttcactgata acaactataa
      421 aataaaagtt atggctgttc tgatacagga aaataaacgt tcattctatg gttacctctt
      481 cctgttatat agaagaattt taaacgacaa gtaagggaat ataaatgaaa ataacaaaaa
      541 ataaaaatgc aactataaaa aacagtcatc agtcggctgc ttttcaatgt ttaccttatc
      601 attt
//
LOCUS       DAZGLJ010000641          597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000641 DAZGLJ010000000
VERSION     DAZGLJ010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.654"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>597
                     /locus_tag="ACHK49_005480"
     CDS             <1..>597
                     /locus_tag="ACHK49_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO9265899.1"
                     /translation="LIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVYTTTQSNEPP
                     AGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAMVNPNLGYSV
                     DEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQQGRRVSLDD
                     ILQRADVVTVGIDGGGLDDLLGMYVTGRDRETREWLGWG"
BASE COUNT          121 a          141 c          204 g          131 t
ORIGIN      
        1 ctgattgatg aactgtggct gtttggcaag cagtacaagg cggaagacat gctacgtgaa
       61 gccatcggcg ggcttgcctc ccgtccggaa gggtttgtgg tgtatacgac cacccagtcg
      121 aatgagccgc cagccggggt gttcagacag aaactgcagt acgcccggga tgtccgtgac
      181 ggcaaaattc atgatccgca ttttctgccg gtgatatttg aacaccctcc tgaaatggtg
      241 gaaagcgggg ctcacctgct gatggaaaac ctcgccatgg tcaatccgaa tctcggttat
      301 tcggtggatg aggcttttct gtaccgggag taccgtaaag cccgggaggc tggtgaggaa
      361 gcatttcgtg gcttcatgtc aaaacatgcc aatgtggaaa ttggtcttgc cctgcgttct
      421 gaccgctggg cgggtgcgga tttctgggag cagcagggca ggcgcgtcag cctggacgat
      481 atcctgcagc gcgctgatgt ggtgacggtg gggattgacg gcgggggcct ggatgatctg
      541 ctgggaatgt acgtgactgg ccgtgacagg gaaacccgcg aatggctggg ctggggc
//
LOCUS       DAZGLJ010000642          593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000642 DAZGLJ010000000
VERSION     DAZGLJ010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.655"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>593)
                     /locus_tag="ACHK49_005481"
     CDS             complement(<1..>593)
                     /locus_tag="ACHK49_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO9265900.1"
                     /translation="SKYNEKVRLIKSDVNLGYFGGLNLGLKGLPRNHPIVVGNNDLVY
                     ESNFTQIISQTTYPDDALVIAPNVITKDGYHQNPHCRKRVSKFRKFLYDIYFSNYIAA
                     LVLTWGSRFLNYLKGGRNNSFDQGRGYIHMGIGACYVLMPSFFKYFDELDNKVFLYGE
                     EAYLAGQLMEVNGKIFYEPDAIVHHEESATLAKVASK"
BASE COUNT          199 a          129 c           74 g          191 t
ORIGIN      
        1 tttagaagca actttcgcca aagttgcact ttcttcatga tggacaatag catcaggctc
       61 atagaaaatc ttcccattta cttccattaa ttggccagcc aaatatgctt cctcaccata
      121 taaaaaaact ttgttatcta attcatcaaa atatttgaag aaggaaggca taaggacata
      181 acaggctcca atacccatat gtatataacc tctaccctga tcaaaggaat tatttcttcc
      241 acctttcaaa taatttaaaa acctgcttcc ccacgttaaa actaaagcag cgatataatt
      301 tgaaaaatat atatcataca aaaatttcct aaacttactt actcgcttac ggcagtgtgg
      361 gttctggtga taaccatcct ttgtaatcac atttggagca ataactaaag catcgtccgg
      421 atatgtagtc tgtgatatta tttgcgtaaa attactctca taaaccaaat cattattccc
      481 aacaactatt ggatgatttc ttggtagtcc ttttaggcca agattcaatc cgccaaaata
      541 tcccaaattc acatcacttt taattaatct gaccttctcg ttatatttac tct
//
LOCUS       DAZGLJ010000643          585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.656, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000643 DAZGLJ010000000
VERSION     DAZGLJ010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.656"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..438
                     /locus_tag="ACHK49_005482"
     CDS             <1..438
                     /locus_tag="ACHK49_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO9265901.1"
                     /translation="PGAPISNKRGQLDLDAWRDFYISYLRRSKNDVPDGDSEDDYEEK
                     LLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCIFALSKLAMALSSTLDSIPLSMQRQ
                     FPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLLDEYIRATTE"
     gene            410..>585
                     /locus_tag="ACHK49_005483"
     CDS             410..>585
                     /locus_tag="ACHK49_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265902.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIA"
BASE COUNT          174 a          123 c          146 g          142 t
ORIGIN      
        1 ccgggtgccc cgatatcaaa taagcgaggg caactggatc tggatgcctg gcgcgatttt
       61 tacatatcgt atctgaggag aagtaaaaac gatgtgcctg atggcgatag cgaagacgac
      121 tatgaggaaa aattgcttat tgccagatgg gaactgacag cagaacaggc tgttacacag
      181 cagttaaaaa atgaggtgtc aaaaggaaaa cttattgaca ccgggttctg tatttttgcc
      241 ctcagtaagc tggcaatggc gttatccagt acgcttgatt ccatcccttt atccatgcaa
      301 cgacagtttc ctgatttaac accgcgccat cttgaccatc tgaaaaccct tattgcgaag
      361 ggggcaaatc agtgtgcgcg ggcgggggat aaattaccgg atttactcga tgaatatatc
      421 agagcaacaa ctgaataata tgatgagtgc tgtcacaaca gcattacagc ccctgataag
      481 ggcattgccg gtgacgccag ttgaatgggc tgatcaaaat tattatctgc ctaaagaatc
      541 ttcatatggt gagggagaat ggaaaacgct gccattccag atcgc
//
LOCUS       DAZGLJ010000644          582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000644 DAZGLJ010000000
VERSION     DAZGLJ010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.657"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(456..575)
                     /locus_tag="ACHK49_005484"
     CDS             complement(456..575)
                     /locus_tag="ACHK49_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO9265903.1"
                     /translation="MVYGVFPNQDGTFTAMTYTKSKTFKTENGARRWLAKNTS"
BASE COUNT          154 a          145 c          123 g          160 t
ORIGIN      
        1 ggtatttcat cagttccgaa gtgatgtgca atcataatta tctccatttt tacaaatgaa
       61 ctttgttgat gcggtgcctg gtgcctccag gtgactgcaa ccagttaaca attacagtcg
      121 gctttcccac ccaaaccaat aaggactaac atgactttta actgtgccgc gtgcgcttag
      181 ccgcattcac cgcatcacaa aattcacttt aaaaagggcg gacatcagtc gaacttcaag
      241 aaaaaactga tgccgccaag actacacaca gcagtgttgt tattcacaac cggaggcgca
      301 ctcccaccat ttaaatttaa cagacaagac cgactcttta tggataccgg aaatgcgcct
      361 tcgtgttgtg cccggtttta tttcaccacc tccgggcttt ggtggcctcg gctatacccc
      421 tacagcaaga atattgaatt aatccaataa atggtttagc tggtattttt ggcaagccag
      481 cgacgggcac cattttcggt tttaaacgtt ttgcttttgg tatacgtcat tgcggtgaac
      541 gtgccgtcct ggtttggaaa cacgccgtac accagagatg cg
//
LOCUS       DAZGLJ010000645          578 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000645 DAZGLJ010000000
VERSION     DAZGLJ010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.658"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..311
                     /locus_tag="ACHK49_005485"
     CDS             <1..311
                     /locus_tag="ACHK49_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO9265904.1"
                     /translation="TIQTLKQTRAMTGEILETWLDGHTLWASVNMISSKEAISSGAEL
                     ATGTVRIWIRYRKDINATSRIKVNTGPLAGRVLNIIGQPLPDAARTRLEILCREGAEK
                     "
     gene            308..>578
                     /locus_tag="ACHK49_005486"
     CDS             308..>578
                     /locus_tag="ACHK49_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO9265905.1"
                     /translation="MTELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRRF
                     DDGLEFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTA"
BASE COUNT          160 a          144 c          165 g          109 t
ORIGIN      
        1 taacgattca gaccctgaaa cagaccagag ccatgacggg tgaaatactc gaaacgtggc
       61 tggacggtca cacactctgg gcaagcgtga acatgatcag cagcaaggag gccatttcat
      121 caggtgcaga gctggcgact ggaactgtaa ggatatggat acgctaccgg aaggacatca
      181 acgccaccag tcggataaag gtcaatacgg ggccgctggc ggggcgtgta ctgaatatca
      241 tcgggcaacc gctgccagat gccgccagga cacgccttga aattctttgt cgtgagggcg
      301 cggaaaaatg acagaactta tcaccctgga agaagtgaaa ctccattgcc gcatcgatgg
      361 cgacgatgaa gaccagttaa tcagcggata cattgccgca tcgcttgagg cgtgccagat
      421 acacataggc aggcgctttg atgacgggct ggagttcacg ccagccataa agattggctg
      481 catgatgttt atcgctcacc tgtatgaaaa tcgccagatt gtcgcggata acgcaaaaac
      541 gcgcgtaccc atgacgatag gcgcactctg gacggctt
//
LOCUS       DAZGLJ010000646          574 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.659, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000646 DAZGLJ010000000
VERSION     DAZGLJ010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.659"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>574
                     /locus_tag="ACHK49_005487"
     CDS             <1..>574
                     /locus_tag="ACHK49_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO9265906.1"
                     /translation="LKIIMEDTSVTENIFKNIEMAIADIKKEYYSHTVDIEKNIHAIW
                     VAGSPPESISDYIKTFLKTYKEFTYYLWVDEKAFGAAKFTSVLKQIAFDLACRTIQQN
                     TPQKNIDFINLYNEIRKKYNNNPSGQQEYLNKLRELYATYQKISTPLKHMFNSFFLEN
                     MIKLQDNFFNYCIVKGVTEINDELRINYLK"
BASE COUNT          225 a           96 c           86 g          167 t
ORIGIN      
        1 tactaaaaat aatcatggag gatacctctg ttacagaaaa tatctttaaa aacatagaga
       61 tggctatagc agatataaaa aaggagtact attcgcatac agttgatatt gagaaaaata
      121 ttcatgccat atgggttgcg ggttccccac ctgaaagcat ctcggactat attaagactt
      181 ttctcaaaac ctacaaagag tttacttact atctttgggt tgatgaaaaa gcatttggag
      241 ccgcgaaatt taccagtgtc ttaaaacaaa ttgcatttga tttagcatgc agaactatac
      301 agcagaatac tccacaaaaa aatattgatt ttattaatct atataatgaa ataaggaaga
      361 aatacaacaa caacccatcg ggacaacaag agtacttaaa caaactcaga gagctttatg
      421 ctacttatca aaaaatatct acccctctga aacacatgtt taattcattt tttctagaaa
      481 acatgattaa acttcaggat aatttcttca actattgcat tgtcaaaggt gttacagaga
      541 ttaatgatga gttacgaata aactacctta aaaa
//
LOCUS       DAZGLJ010000647          573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.660, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000647 DAZGLJ010000000
VERSION     DAZGLJ010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.660"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(109..333)
                     /locus_tag="ACHK49_005488"
     CDS             complement(109..333)
                     /locus_tag="ACHK49_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265907.1"
                     /translation="MKKLQVTVKPFQGTIPFRVLQHGRVLLEEVFRGKCTECYSRTYE
                     VNATHEEFTVECVMNTDKCRMVSAELQPVC"
     gene            complement(330..518)
                     /locus_tag="ACHK49_005489"
     CDS             complement(330..518)
                     /locus_tag="ACHK49_005489"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFO9265908.1"
                     /translation="MREATGTSGVDNATGPRLADTAERDYFILRERLMTMQKQVEGAQ
                     DYIRTQCTKQAFYYPEDV"
     gene            complement(482..571)
                     /locus_tag="ACHK49_005490"
                     /pseudo
     CDS             complement(482..571)
                     /locus_tag="ACHK49_005490"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap.; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
BASE COUNT          134 a          155 c          134 g          150 t
ORIGIN      
        1 gtttttgatt atccccgcac actctcgcag aggagtctcc ctgtcgggct gcggtctctg
       61 ttaatgcagg aatacggcga caataccgcg catggataat aaggtcgctc aacacactgg
      121 ctgtaattca gcggatacca ttcggcattt atcagtattc atcacacact caacggtgaa
      181 ttcttcatgc gtggcattca cttcatatgt tcgtgaataa cattcagtgc atttacctct
      241 gaacacctct tcaagcagaa cacggccatg ttgcaaaaca cggaacggaa ttgttccctg
      301 aaaaggtttt accgttacct gtaatttctt catacatcct ccggataata aaaagcctgc
      361 ttagtacact gagtgcggat atagtcctgt gccccttcca cctgcttctg cattgtcatc
      421 aaccgttctc tgaggatgaa ataatcccgt tcagcggtgt ctgccagtcg ggggccggtt
      481 gcattatcca cgccggaggt gccggtggct tcacgcacgg taccggggca ggtggcgttg
      541 atccgcaggc gcttacgacc agcggcaaca tca
//
LOCUS       DAZGLJ010000648          571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.661, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000648 DAZGLJ010000000
VERSION     DAZGLJ010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.661"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(4..546)
                     /gene="yehD"
                     /locus_tag="ACHK49_005491"
     CDS             complement(4..546)
                     /gene="yehD"
                     /locus_tag="ACHK49_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9265909.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKEIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT          174 a          124 c           98 g          175 t
ORIGIN      
        1 ttattaatcc tgcgccacac gcataaccag agaagataac acatcacctg attgcactga
       61 gttatctgtc agtttcaggt attttgcttt aaacggaata gcatattgac cttccgctgt
      121 tttataactc tcaatgttaa aaggtgcaga aacatccagg acatcgctgt tatcacctaa
      181 atagagatag taacccacgg tatcgttgct actggctatc tccttatcgt taccggttat
      241 tttatcgttg gtaatcaacg agagttttac accagcaact tctttcgggc agctaatttt
      301 taacatgctg ctggttacac cgactgcatc atcataggta gaatatttag ctttaccttc
      361 aaagacagtg gttggtacag tcggcatatt aatacttact gaatcaccac ccgcttcgaa
      421 tttacacgga gattctgcaa tattcccctt aatagttaat gttccggtat caacgtctgc
      481 agcaaatact ccagcgctca taaaaaaaga agaaaagaca gcggcagcaa taattgaacg
      541 tttcataata tgttccttaa taaaatttat t
//
LOCUS       DAZGLJ010000649          568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.662, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000649 DAZGLJ010000000
VERSION     DAZGLJ010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.662"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(369..473)
                     /gene="speFL"
                     /locus_tag="ACHK49_005492"
     CDS             complement(369..473)
                     /gene="speFL"
                     /locus_tag="ACHK49_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO9265910.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            complement(452..>568)
                     /locus_tag="ACHK49_005493"
     CDS             complement(452..>568)
                     /locus_tag="ACHK49_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO9265911.1"
                     /translation="YDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA"
BASE COUNT          182 a          106 c          121 g          159 t
ORIGIN      
        1 gatgagtgga aaaacaatcc gggcaagaac gactaacggc aatttttaat tctgacattt
       61 tgttcatctc tttatttcag gtaatagcag gtcctcaatt tctcaaagga gaaatcgatc
      121 acgccggaaa aaaagcaaag ctccaccgct gataaataaa tcagtagttt gctttttgca
      181 gatgcaataa cccagaccag cccggtaact ggctatactc gtcatacctc aagttgcaag
      241 tgctgcgact cgaattattt agagtttaaa agggttagca gtatatgagc accgaggaat
      301 acaggtatta acctgtaatg cttaaagtca gggagacctg tacgttgggg tgccctttag
      361 tcagaagact agcgggcatt aaagaaatga aagtcaaaac tattacgatg ggcaaacttc
      421 atgatatggg ttgtccgcct tatatgaggc attaagcggt tattattttc catttacctt
      481 tacctcttgc tgtgtcagtt gaaggtagag ttattctatc catgaaatgg ctataatctg
      541 tgaagaagat caacaaaaaa cgatcata
//
LOCUS       DAZGLJ010000650          566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.663, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000650 DAZGLJ010000000
VERSION     DAZGLJ010000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.663"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            125..517
                     /gene="ydeI"
                     /locus_tag="ACHK49_005494"
     CDS             125..517
                     /gene="ydeI"
                     /locus_tag="ACHK49_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO9265912.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT          169 a          111 c          137 g          149 t
ORIGIN      
        1 aatatttgac agctagtggg ctgattttaa atgccttata acaattggca gtattcttat
       61 ttggcaatta taaaaatagc aaccagactt aataagtcaa accaaacgca attaaggagt
      121 tagtatgaaa tttcaggcca ttgtattagc aagttttctt gtcatgcctt atgcgttggc
      181 agatgaccag ggcggtttaa aacaggatgc agcgccaccg ccgcctcatg caatagaaga
      241 tggctatcgc ggaaccgatg atgcaaaaaa aatgaccgtt gatttcgcaa aaaacatgca
      301 cgatggcgct tcggtttcac tacgcggtaa tttgatttct cacaaaggag aggaccgtta
      361 cgtttttcgc gataagagcg gtgaaattaa tgtcgttatt cctgcggccg tctttgatgg
      421 acgagaagtt cagccggacc agatgatcaa cattagcggc agtctggata agaaaagcgc
      481 gccggccgtc gttcgggtca ctcatttgca gaaataaaaa taaattagcc tgatggcctg
      541 aactactggg ccattagggt attggg
//
LOCUS       DAZGLJ010000651          565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.664, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000651 DAZGLJ010000000
VERSION     DAZGLJ010000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.664"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>565
                     /locus_tag="ACHK49_005495"
     CDS             <1..>565
                     /locus_tag="ACHK49_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO9265913.1"
                     /translation="PGIPRPALDSWDAEYMQENRWDEESASFIPVEPESDPMNVNFDK
                     LSLEVQNAVLVKFGTCENITVDMAIDAQELLQEDVATFDGHIVEALMKTPEINAMYPE
                     RKLFAIGWVKHKCNPGAKWPEIQAELRNWKKRQDAERKETGKYTSVVDLASARVNQQN
                     TENSAGKINPVTAAICREYKQTWKTLD"
BASE COUNT          172 a          136 c          138 g          119 t
ORIGIN      
        1 aacccggtat accccgccct gccctcgatt cctgggatgc tgaatatatg caggaaaacc
       61 gctgggatga ggaatccgct tcctttattc cggtcgaacc agaatccgat ccgatgaacg
      121 tcaattttga caagctgtcc cttgaagtac agaacgcggt cctggttaag ttcggtacat
      181 gtgaaaacat caccgttgat atggcgattg acgcgcagga attactgcag gaagacgttg
      241 ctacctttga cgggcatatc gttgaagcac tgatgaaaac gcctgaaatt aacgctatgt
      301 atccggaacg caaactgttc gctatcggat gggttaaaca caaatgtaat ccgggtgcca
      361 aatggcccga aattcaggct gaattacgta actggaaaaa acggcaggac gcagagcgca
      421 aagagactgg aaaatacacg tctgttgttg atctcgccag cgccagagtc aatcaacaga
      481 acactgaaaa ctcagcagga aaaatcaacc cagtcactgc cgccatttgt cgcgaataca
      541 agcagacatg gaaaacgctg gatga
//
LOCUS       DAZGLJ010000652          565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.665, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000652 DAZGLJ010000000
VERSION     DAZGLJ010000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.665"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..277)
                     /gene="tnpB"
                     /locus_tag="ACHK49_005496"
     CDS             complement(<1..277)
                     /gene="tnpB"
                     /locus_tag="ACHK49_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9265914.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQ"
     gene            complement(274..>565)
                     /locus_tag="ACHK49_005497"
     CDS             complement(274..>565)
                     /locus_tag="ACHK49_005497"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9265915.1"
                     /translation="PGISISKLALENGINANLLFKWRQQWREGKLLLPSSESPQLLPV
                     TLDAAAEQPESLAEDPETLSISCEVTFRHGTLRFNGNVSEKLLTLLIQELKR"
BASE COUNT          120 a          153 c          161 g          131 t
ORIGIN      
        1 gctgcgcctg cgtaaggaac actttgccat cacgggctga cggccaggcg aagcgcccac
       61 gctccagccg tttggtcagg aggcacagtc cgtcaccggt ggaccacagc agtttaacct
      121 gactgccgct gcggccccgg aaaatgaaaa catggccgga catgggatcg tctttcagcg
      181 ccgtttgtac tttcgcagcc aggccgttga agccatttct catatcggtg ataccggcaa
      241 ccagccaaat tttggtcccg gaaggtaacg ggatcatcgc ttcagttcct gtatcagcag
      301 agtcaggagc ttttcgctga cattgccatt gaagcggagc gtcccgtgcc ggaacgttac
      361 ctcacagctg atactgaggg tttccgggtc ctctgcgagc gattctggct gttcggcagc
      421 tgcatcgaga gtcacaggaa gtagctgggg gctctctgaa gaaggtaata gcagctttcc
      481 ctcgcgccat tgttgtcgcc atttgaacaa cagattggcg ttaatgccat tttcaagagc
      541 aagttttgag atggatatcc cgggt
//
LOCUS       DAZGLJ010000653          559 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.666, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000653 DAZGLJ010000000
VERSION     DAZGLJ010000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.666"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>559
                     /locus_tag="ACHK49_005498"
     CDS             <1..>559
                     /locus_tag="ACHK49_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO9265916.1"
                     /translation="NRHRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLD
                     AIENMTSGGLIYAVSGVPGLIAANSITNSAYQFGYDMDAIVGGAHNGAADAMRHCYLM
                     CRMTKTFGSTIADVIGKNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSDACI
                     EKYNTGQLFGLDGIKADNPIKAKQGS"
BASE COUNT          170 a           97 c          147 g          145 t
ORIGIN      
        1 aaccgtcacc ggtactacga tccgttgcag gggcggtata tcaccccgga cccgattggg
       61 ttgagaggtg gatggaatat gtatcagtat ccgttgaatc ccatacaagt gatagaccca
      121 atggggttag atgcgattga gaatatgaca tcaggtggac taatttatgc cgtatctggt
      181 gtacctggat tgattgctgc aaacagcatt actaacagtg cttaccagtt cggttatgat
      241 atggatgcta ttgttggcgg agctcataat ggggccgccg atgcaatgag acattgttac
      301 ttgatgtgtc gaatgactaa gacatttgga tcaacaatag ctgacgtgat aggtaaaaat
      361 catgaggcgg caggggatag acaaggtcag ccagctaaag aaagaatcat ggatcttaaa
      421 aataacactg tcggtattgc ttgtggcgat ttttctgcca aatgtagcga tgcatgtatt
      481 gaaaaatata acactgggca actcttcggg ttagatggta taaaagcaga taatccaata
      541 aaagcaaagc aagggagtt
//
LOCUS       DAZGLJ010000654          559 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.667, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000654 DAZGLJ010000000
VERSION     DAZGLJ010000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.667"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            175..528
                     /locus_tag="ACHK49_005499"
                     /pseudo
     CDS             175..528
                     /locus_tag="ACHK49_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          165 a          112 c          143 g          139 t
ORIGIN      
        1 tagcattcgg cgtataagtt gacagagaag gagtatctca gcaggagctt aaagtgtggt
       61 tttaggcaga atttgattga tcctccattt ggggcatgtg actaatcgat atagcttcct
      121 gaacggaaaa agtgttttcc cttccaatat aaactattcg acaataatat aaatcaatta
      181 cagaataaaa ctctgtataa acatgcaaaa atggtaaagg cgttcgacta tatcctgaat
      241 cactggaatg cgctgaacga gttctgtcgt gacggctggg tggaaataga caacaacatc
      301 ggtgaaaacg cgttacgatc ggtggcggtt ggaagaaaaa attatctctt cttcggctca
      361 gacaaggggg gagaaagcgc ggcgatcatc tacagtctgc tggtcacctg caaactgaac
      421 gaagtggagc cggaggactg gttgcgcgaa gtgatcgtga agctcaatga ctggccgtcg
      481 aaccgtgtgc atgaactgct gccctggaac tgctcaactg taaaataatc cttacgctac
      541 gtacttctcg gggcgctta
//
LOCUS       DAZGLJ010000655          553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.668, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000655 DAZGLJ010000000
VERSION     DAZGLJ010000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.668"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..553
                     /locus_tag="ACHK49_005500"
                     /pseudo
     CDS             1..553
                     /locus_tag="ACHK49_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          180 a           87 c          141 g          145 t
ORIGIN      
        1 aaccggaacc ggtactacga tccgttgcag gggcggtata tcactcagga cccgataggg
       61 ctggaggggg ggatggagtc tgtatgcgta cccgctgaat ccggtgaatg gtattgatcc
      121 attagggtta agtcccgcag atgtagcgct aataagaaga aaagatcaac taaaccatca
      181 aagagcatgg gatatattat ctgatactta tgaagatatg aagagattaa atttaggtgg
      241 gactgatcaa tttttccatt gtatggcatt ttgtcgagtg tctaaattaa atgacgctgg
      301 tgttagccga tcggcgaaag ggctgggtta tgaaaaagag attagagatt acgggttaaa
      361 tctgttcggt atgtacggca gaaaagtaaa gctatcccat tctgaaatga ttgaagataa
      421 taaaaaagac ttggctgtaa atgaccatgg gttgacatgt ccatcaacaa cagattgctc
      481 agatagatgt agtgattata ttaatccaga gcataaaaaa acgataaagg ctttacaaga
      541 tgctggctat ctc
//
LOCUS       DAZGLJ010000656          552 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.669, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000656 DAZGLJ010000000
VERSION     DAZGLJ010000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.669"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..109)
                     /locus_tag="ACHK49_005501"
                     /pseudo
     CDS             complement(1..109)
                     /locus_tag="ACHK49_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            86..>552
                     /locus_tag="ACHK49_005502"
     CDS             86..>552
                     /locus_tag="ACHK49_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO9265917.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATRDPEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKG"
BASE COUNT          126 a          144 c          163 g          119 t
ORIGIN      
        1 cgaccagcca ctggcagatg gccatcctgt gatttgattc cgcttccggc tttttaactc
       61 tgttcctcta cacgggagaa attcgatgtc gattaaacat tatgatgttg tcagggcggc
      121 gtcgccgtca gaccttgcgg aaaagctgac acaaaaactg aaggaggggt ggcagccatt
      181 tggcagcccg gtggccatca cgccttatac tctgatgcag gccattgcgg cggaaggtga
      241 tgtcaccaca cctgtgttgg tgaagccgtc ggatggagaa ggcacagtaa tcagcgccac
      301 cagagacccg gagtattact ttgttgtggt tctggcgggg cagtcaaaca gcatggcata
      361 tggtgaaggc cttccgctgc cggagacata tgaccgtccg gacccgcgca ttaagcagct
      421 ggcgcgtcgc agtacggtga caccgggcgg tgtcgcctgt aaatataacg acatcattcc
      481 ggcggaccat tgtctgcatg atgtgcagga catgagccgc cttaaccatc cgaaagcgga
      541 cctgtcaaag gg
//
LOCUS       DAZGLJ010000657          550 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.670, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000657 DAZGLJ010000000
VERSION     DAZGLJ010000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.670"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..542
                     /locus_tag="ACHK49_005503"
     CDS             <1..542
                     /locus_tag="ACHK49_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO9265918.1"
                     /translation="KKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKPGDE
                     DLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQT
                     DLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPS
                     KDATPGNVYAQADYKIAYE"
BASE COUNT          168 a          119 c          122 g          141 t
ORIGIN      
        1 ttaaaaagtc acttcttgtt gctggcgtag caatgattct ttccaataac gtatttgctg
       61 acgaaggtca cggcattgtt aaatttaaag gtgaagttat ctctgctcca tgttcaatta
      121 aaccaggtga cgaagactta actgtaaatc tgggtgaagt agcagatacc gttctgaaaa
      181 gcgaccagaa atctttggct gagcctttca ctattcacct gcaagactgc atgttgagcc
      241 agggtggaac cacttattca aaagcgaagg ttactttcac cacagcgaat actatgacgg
      301 gtcagactga cctgctgaaa aacaccaaag aaactgaaat cggtggtgcc acaggcgttg
      361 gcgtacgtat tctggatagc cagagcggcg aagtgacttt aggtactccg gtagtaatta
      421 cattcaacaa tactaacagc tatcaggaac tgaacttcaa agctcgtatg gaatccccaa
      481 gcaaagatgc taccccgggc aacgtttacg ctcaggctga ctataagatt gcttacgagt
      541 aatcacatta
//
LOCUS       DAZGLJ010000658          548 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.671, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000658 DAZGLJ010000000
VERSION     DAZGLJ010000658.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.671"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            29..217
                     /gene="dicB"
                     /locus_tag="ACHK49_005504"
     CDS             29..217
                     /gene="dicB"
                     /locus_tag="ACHK49_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO9265919.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            214..402
                     /locus_tag="ACHK49_005505"
     CDS             214..402
                     /locus_tag="ACHK49_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO9265920.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
BASE COUNT          175 a          110 c          114 g          149 t
ORIGIN      
        1 cacaaaattc attgttgagg acgcgataat ggaaacgtta ttaccaaacg ttaatacgtc
       61 tgaaggttgt tttgatattg gtgttctgct cagtaaccgg gagtttactg aagatgccat
      121 taatatgagg aaatatgagc cttatctgct caatgataat tccatacttt cccgaattgc
      181 tcttcttgaa cttggtattt tcggagaacg tcaataactt cagcatttgc actgatgatg
      241 acggtttttc ttataacggg tgaatcacag aatgtgatta ccggaattta tgccagtaaa
      301 gaatcctgcc tccaggcaag agacgagcaa aaaatttctg gtgaatgcct cccggtaaaa
      361 aaagtatcgc tgtacctgaa taacgaaaca ccggctggat aaccctccag ccatattaac
      421 accataccaa cggattaaaa atgccagcaa tggcagggat tcgttcaccc tgaaatctgt
      481 aatgaggtaa aacaaaatga gtaaagtctt tatttgcgcc gccattccgg acgaacaggc
      541 aataaagg
//
LOCUS       DAZGLJ010000659          546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.672, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000659 DAZGLJ010000000
VERSION     DAZGLJ010000659.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.672"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..239
                     /locus_tag="ACHK49_005506"
     CDS             <1..239
                     /locus_tag="ACHK49_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO9265921.1"
                     /translation="MMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVPTSLEK
                     QAVQLLERELFADGNATVSNEMKGKLELVVADYL"
     gene            283..>546
                     /locus_tag="ACHK49_005507"
     CDS             283..>546
                     /locus_tag="ACHK49_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFO9265922.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVTPEQLARLEEEPCLTVRILQTSADDAGSVAGVVHAV"
BASE COUNT          113 a          139 c          178 g          116 t
ORIGIN      
        1 acatgatgca gtcaccgctg acgctggatg cactgtggca cggctggtca gccatgcgcg
       61 aatttaccgc tgacggtggc cgcaaactcg gcattaaacc cacccatatt gttgtcccca
      121 cctcgctgga aaaacaggcg gtgcagctgc tggagcgtga actgttcgca gacggaaacg
      181 caaccgtctc taacgagatg aagggtaagc tggagctggt tgtcgcggat tatctgtaaa
      241 cggtgagccg ggcttacggc ccggcccctg cggagggcaa atatgaatga acatcacact
      301 gtggcagcaa cagatgattc aggtcttcag gtcagtggtg acaattctgt cacggtgctg
      361 gcagaagtgc gttgcagtcg ttcggcgttt cgtcgtgcgg ggttcctgtt cacgcgtgga
      421 cgtcagcagg ttgaggtcac cccggagcag cttgcccgac tggaggagga gccatgcctc
      481 actgtgcgaa tactacagac gtctgctgat gatgcgggga gcgtggcggg tgtggttcat
      541 gcagtg
//
LOCUS       DAZGLJ010000660          543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.673, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000660 DAZGLJ010000000
VERSION     DAZGLJ010000660.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.673"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..457)
                     /locus_tag="ACHK49_005508"
     CDS             complement(<1..457)
                     /locus_tag="ACHK49_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO9265923.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATRDTEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKAD"
     gene            434..543
                     /locus_tag="ACHK49_005509"
                     /pseudo
     CDS             434..543
                     /locus_tag="ACHK49_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          117 a          159 c          140 g          127 t
ORIGIN      
        1 ggtccgcttt cggatggtta aggcggctca tgtcctgcac atcatgcaga caatggtccg
       61 ccggaatgat gtcgttatat ttgcatgctg caccgcccgg tgtcaccgta ctgcggcgcg
      121 ccagctgctt aatacgcggg tccggacggt catatgtctc cggcagcgga aggccttcac
      181 catatgccat gctgtttgac tgccccgcca gaaccacaac aaagtaatac tccgtgtctc
      241 tggtggcgct gattactgtg ccttctccat ccgacggctt caccaacaca ggtgtggtga
      301 catcaccttc cgccgcaatg gcctgcatca gagtataagg cgtgatggcc accggactac
      361 caaacggctg ccagccctct ttcagtttgt gtgtcagctt ttccgcaagg tctgacggcg
      421 acgccgccct gacaacatca taatgtttaa tcgacatcga atttctcccg tgtacaggaa
      481 cagagttaaa aagccggaac cggaatcaaa ttacaggatg gccatctgcc agtggctggt
      541 cgt
//
LOCUS       DAZGLJ010000661          538 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.674, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000661 DAZGLJ010000000
VERSION     DAZGLJ010000661.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.674"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(134..343)
                     /locus_tag="ACHK49_005510"
     CDS             complement(134..343)
                     /locus_tag="ACHK49_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO9265924.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVSLEDAMLCW
                     GEDEDKVESDPFAALEDALSFAAMS"
     gene            complement(439..>538)
                     /locus_tag="ACHK49_005511"
     CDS             complement(439..>538)
                     /locus_tag="ACHK49_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO9265925.1"
                     /translation="REQVLAVYGPVHARLLRQALELIADAESARKK"
BASE COUNT          122 a          176 c          124 g          116 t
ORIGIN      
        1 atacgggaag cacgctggag ctccgactgg aatgactgtg aatttgccga aattttgata
       61 atcagttcac gtaacgtcgc cattcacctt tctccgggca aaaaaacctg ccacagcagg
      121 ttttcatcat tatttatgac attgctgcaa agctcagcgc gtcttccagc gccgcaaacg
      181 gatctgactc tactttatcc tcatcctcgc cccagcagag catggcgtcc tccagtgaaa
      241 cattcatccc ctgcgccccg aaaaccgctt tcacgatctg cgcattacgg atatccccgc
      301 gctcatcgcc cagcggggac accctgtcga actccatcca catcatcgcc tcgctcgcac
      361 tcagactgtg gcccagttcg gataaggtgc gccccagacg gagcgcaagt cgcatcagaa
      421 agcgaatttc cgggcgggct acttttttct ggccgactct gcatcagcga tcagttccag
      481 tgcctgacgc agcaaccggg catgtaccgg accatagacg gccagcacct gctcacgg
//
LOCUS       DAZGLJ010000662          520 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.675, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000662 DAZGLJ010000000
VERSION     DAZGLJ010000662.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.675"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>520
                     /locus_tag="ACHK49_005512"
     CDS             <1..>520
                     /locus_tag="ACHK49_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265926.1"
                     /translation="IRNKFSHSWENISIEDQDISQQIKALSFSRIDFECPKDNYQKIK
                     KSISCLLIEIKITTSQIKKKHLKARLVGSNVNIGFSGKYEEQVNDIKKNIESIKNDLT
                     SHDKNIKSFAVHTANLLIERLSYVQFNHDDLDVFSDQLVDILEIKYQLLNLLGINGVT
                     DLSQKEKEKLKKS"
BASE COUNT          217 a           71 c           78 g          154 t
ORIGIN      
        1 ataagaaata aattctctca cagctgggaa aacatttcaa ttgaggatca ggatatttct
       61 cagcaaatta aagcgttaag cttcagtcgt attgattttg aatgcccaaa ggataactat
      121 caaaaaataa aaaaatccat atcgtgttta ctaattgaaa ttaagataac cacatctcaa
      181 atcaaaaaga aacacctcaa agcaaggtta gttggttcaa atgttaacat aggattctcc
      241 ggtaagtatg aggaacaagt taatgatatt aagaagaaca tagaatccat aaaaaatgat
      301 ttaacttcac atgataaaaa tataaaatca tttgcagtac atactgctaa tttactcatt
      361 gaacgtttat catatgtgca atttaaccat gatgacctag atgttttttc tgaccaatta
      421 gtagatatcc tagaaataaa ataccaattg cttaatttat taggaattaa tggagttaca
      481 gatttaagtc aaaaggaaaa ggaaaagttg aaaaaaagtt
//
LOCUS       DAZGLJ010000663          520 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.676, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000663 DAZGLJ010000000
VERSION     DAZGLJ010000663.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.676"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>520
                     /locus_tag="ACHK49_005513"
     CDS             <1..>520
                     /locus_tag="ACHK49_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265927.1"
                     /translation="CTANNYLKQAWNNIKNEHEKNQKYSITLFENTLVCFMRLYKEIR
                     RQKAEDYIPCLECDSLEKEFEEMQNDNDLSLFLRTLRTNDTETYSGVSEGITYTIQYV
                     RDIDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLDDENMSIQPE
                     WINTPGISDLDTG"
BASE COUNT          186 a           66 c          105 g          163 t
ORIGIN      
        1 tgcactgcaa ataattattt gaaacaagcc tggaacaata taaaaaatga gcacgaaaaa
       61 aatcaaaaat attcaatcac gctttttgaa aacacactgg tgtgttttat gcggttgtat
      121 aaagaaatca gacgtcaaaa agctgaagac tacattccat gcctggaatg cgattcacta
      181 gaaaaagaat ttgaggaaat gcagaatgat aatgatttat cattattttt gagaacattg
      241 cgtactaatg atacagaaac ttattcaggg gtttcagaag gtattacata tactatacaa
      301 tatgttcgag atattgatat tgttagagtg tccttgccgg gcagaggttc agagtctatc
      361 acagatttta aaggttatta ttggtatggc tttatggaat atattgaaaa cattaatgcg
      421 tgtgatgatg ttttttctga gtattgttta gatgatgaaa atatgagtat ccagccagaa
      481 tggataaata cgccgggaat atctgattta gatactggta
//
LOCUS       DAZGLJ010000664          515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.677, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000664 DAZGLJ010000000
VERSION     DAZGLJ010000664.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.677"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>515
                     /gene="nleB"
                     /locus_tag="ACHK49_005514"
     CDS             <1..>515
                     /gene="nleB"
                     /locus_tag="ACHK49_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFO9265928.1"
                     /translation="SMYDKKLSDIYLENIHKQESYPASERDNYLLGLLREELKNIPEG
                     KDSLIESYAEKREHTWFDFFRNLAMLKAGTLFTETGKTGCHNISPCSGCIYLDADMII
                     TDKLGVLYAPDGIAVHVDCNDEIKSLENGAIVVNRSNHPALLAGLDIMKSKVDAHPYY
                     DGLGKGIKRHF"
BASE COUNT          169 a           78 c          114 g          154 t
ORIGIN      
        1 tcagcatgta tgataaaaaa ctatctgata tttaccttga aaatatccat aaacaagaat
       61 cataccctgc cagtgagagg gataattatc tgttaggctt attaagagaa gagttaaaaa
      121 atatcccaga aggtaaggac tctttgattg agtcatatgc agaaaaaaga gaacatactt
      181 ggtttgattt tttcaggaat ttggccatgt tgaaggctgg aactttgttt acagagacgg
      241 gaaaaactgg atgccataac atatcgccct gtagcggatg tatatatctt gatgccgaca
      301 tgattattac cgataaatta ggagtcctgt atgctcctga tggtatcgct gtgcatgtag
      361 attgtaatga tgagataaaa agtcttgaaa atggtgcgat agttgtcaat cgtagtaatc
      421 atccagcatt acttgcaggc ctcgatatta tgaagagtaa agttgacgct catccatatt
      481 atgatggtct aggaaagggt atcaagcggc atttt
//
LOCUS       DAZGLJ010000665          513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.678, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000665 DAZGLJ010000000
VERSION     DAZGLJ010000665.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.678"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..176)
                     /locus_tag="ACHK49_005515"
     CDS             complement(<1..176)
                     /locus_tag="ACHK49_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO9265929.1"
                     /translation="MRKLYAAILSAAICLTVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYE"
     gene            complement(244..>513)
                     /locus_tag="ACHK49_005516"
     CDS             complement(244..>513)
                     /locus_tag="ACHK49_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265930.1"
                     /translation="GAKHEREHTDIYSSCRLIVRKNGAEIYNCTALDNTLIYSGVIDM
                     PAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVVMKKSTAGISIS"
BASE COUNT          134 a          134 c          113 g          132 t
ORIGIN      
        1 aattcataac ggtatttcac gttaatcccg ttaagattat cgctaccggg agcgttcgtc
       61 cgggcatgaa gataccccgc gctcagcgtg gcctgctgct cagacgccca tgcaggcgca
      121 ccggatacgg tcagacaaat ggctgcggac aaaatggcgg cataaagttt acgcataatt
      181 acctctcgct tttctgcaat aaaaaaaggc gtcatttctg acgcccgttc tgggttataa
      241 aattcagctg atactgatac ctgctgtgga ttttttcatc accacaacca gcagatcgct
      301 gatacttgct gtgggatacc agttatttac cagccatgct gacaccgaaa actccagcgt
      361 catgtgaccg tgaccggcag gcatatcaat aacgccactg taaatcagcg tattatccag
      421 cgcggtacag ttataaattt cagcaccgtt tttccgcact atcagacggc atgaggagta
      481 aatatcagta tgctctctct catgtttagc gcc
//
LOCUS       DAZGLJ010000666          513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.679, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000666 DAZGLJ010000000
VERSION     DAZGLJ010000666.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.679"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(158..454)
                     /locus_tag="ACHK49_005517"
                     /pseudo
     CDS             complement(158..454)
                     /locus_tag="ACHK49_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          116 a          131 c          108 g          158 t
ORIGIN      
        1 gctgtttaaa ttcgggagga taattagggc agccttttcg cctgccggga gtcacatttt
       61 tctgcatatc tgatactttg gttcccacta cttatttgtt ggacaccact ttgtctaatt
      121 cgtcagattc tgaccagacg gttcaggctg tacgcttacg attacgctga acttcacgtg
      181 agatcgtcga aggactgcga ttcagcgcag tagctatcgc acgaatgctc attttggctg
      241 acaaaccagc tcgtatctcc tcgcgctcag acagtgtcag gtgagctaca gcccgcttac
      301 gctcatgggg ttttatgccg ccagtatccc ttaacatagt gaagatcgtt ccgggttttg
      361 aacccaggat attcgctatt tcactgaagc ctgttccgtt cttccatagt tcaaaaacag
      421 aggctttttc ctctgctgta aatgttcgtc tcattcaaaa aacctccgca accccatgtt
      481 ttcacataac tgttgcgttg accaattgaa tct
//
LOCUS       DAZGLJ010000667          510 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.680, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000667 DAZGLJ010000000
VERSION     DAZGLJ010000667.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.680"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            14..>510
                     /locus_tag="ACHK49_005518"
     CDS             14..>510
                     /locus_tag="ACHK49_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265931.1"
                     /translation="MLVEPEPKNMRALPSGAVPAVRQPLAEDKSLLPFFSDERVIRAA
                     GGAGALSDWLLRHVKSCQWPHGDYHHSETVIHSYGAGAMVLCWHCDNQLRDQTSESLE
                     QLTQQNLTAWMIDVIRHVMNGTQERELSLAELSWWAVCNQVVDALPEAVSRRSLGLPA
                     EKIRSV"
BASE COUNT          112 a          133 c          151 g          114 t
ORIGIN      
        1 tcatcgcggc agggtgctgg tggagccgga accgaaaaac atgcgggcgc tgccatctgg
       61 agcggttcct gctgttcgcc agccgctggc ggaagataaa tcactgctgc catttttcag
      121 cgatgagcgg gtgattcgtg cagctggcgg cgctggtgca ctgtctgact ggttattacg
      181 tcacgtgaaa tcctgccagt ggccacacgg cgattatcat cacagcgaaa ccgttattca
      241 cagttacggt gctggcgcaa tggtgttgtg ctggcactgc gacaaccagc tgcgcgacca
      301 gacctccgaa tcacttgagc aacttactca acaaaatctg acagcctgga tgattgacgt
      361 catacgccat gtaatgaatg gcacgcagga gcgggaatta tcgctggctg aattatcctg
      421 gtgggcagtc tgcaatcagg tggtggacgc attacctgag gcagtatcgc gtcgctctct
      481 gggattaccg gcggaaaaaa tccgctccgt
//
LOCUS       DAZGLJ010000668          509 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.681, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000668 DAZGLJ010000000
VERSION     DAZGLJ010000668.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.681"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..106)
                     /locus_tag="ACHK49_005519"
                     /pseudo
     CDS             complement(1..106)
                     /locus_tag="ACHK49_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          141 a          120 c           89 g          159 t
ORIGIN      
        1 caaaaatagt caatagtagg atgcctgaca atttatgctc tactttccag gcttatctgt
       61 aatcgggaat aatagaaata tgttccatca attttttaag ttccatttgt tcaccttatt
      121 tatgtaagga gtatttgatc atgtataagc aataaaaaac agcttcaggt aatgaggaat
      181 atctcaattt ttaaacataa aatgcgaatt atttagtaca aaaagtgaac ttttgatgat
      241 ctttccctaa tctcacatag gatcagatct cgtctgatcg ttccccctgc gctcactacc
      301 atggctcttt accacagcag ccggggttgt ttgggaccga cttccgacag cagatcccgg
      361 tgggcctgcc accatctctt cacaagctta tgcttattac atgcaaaacg tcccgttttg
      421 catctttgtg cctacggcat ccctgtgatc tttccctgaa tctgcccctg agaacctgaa
      481 tcaaacgtct gactcgcaga accatctgt
//
LOCUS       DAZGLJ010000669          498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.682, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000669 DAZGLJ010000000
VERSION     DAZGLJ010000669.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.682"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            243..>498
                     /locus_tag="ACHK49_005520"
     CDS             243..>498
                     /locus_tag="ACHK49_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265932.1"
                     /translation="MKVRSIGFTINNNNKNINTVDVMNAFINASNREHSRTDYTRKIL
                     ISDVNDFYYGLVVTFRNQKKNCKSQFVDGKFQLKIEDLQGS"
BASE COUNT          175 a           94 c           89 g          140 t
ORIGIN      
        1 tgacatacca ataactcttg tctaaaaagc cagcaagata aaaagccagt attcacgacc
       61 accagcgtgt ttactgtact gcacaaggtt tacaggtaca aaaaaaccgc tcagcggcgg
      121 gtttaagttg tgtggcgaag taaccactct taacacgata taatactttt tgcgtacgcg
      181 ttaggatttt tataaactat gcgtccccat tctcgctatc tttagtcggt cctggaatac
      241 acatgaaagt tagaagcata ggatttacaa taaataacaa taacaaaaat attaataccg
      301 ttgacgtaat gaatgctttt atcaacgcat caaacagaga acacagtcgc acagactata
      361 ctcgtaaaat tctcatttcg gatgtgaatg atttctatta tggattagtt gtcacattca
      421 gaaaccaaaa aaagaactgt aagtcgcaat ttgttgatgg taaattccag cttaaaattg
      481 aagatcttca aggaagtg
//
LOCUS       DAZGLJ010000670          496 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.683, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000670 DAZGLJ010000000
VERSION     DAZGLJ010000670.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.683"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>496)
                     /locus_tag="ACHK49_005521"
     CDS             complement(<1..>496)
                     /locus_tag="ACHK49_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFO9265933.1"
                     /translation="KFNNYLFRWQGLDGSEILMHNFPEDTYDSRARARSLEYIEQNYN
                     EKEICPYAFMVYGVGDGGAGPGEEHIERLTRIRNIDGLPHVDFSRVDKFFTHADAFRE
                     SLPIISGELYFEAHQGCFTSESATKAHNRIMENKLHDAEFFITITNNMTSILRSEFDE
                     IWKAM"
BASE COUNT          148 a          104 c          101 g          143 t
ORIGIN      
        1 catcgctttc catatttcat caaactcact gcgaagaatt gacgtcatat tattagtaat
       61 agttataaaa aattcagcat catggagttt attttccata attcgattat gggctttagt
      121 tgctgactct gatgtaaagc acccttgatg agcctcgaag taaagttcac ccgaaataat
      181 gggcaaggat tcgcgaaagg cgtcggcgtg agtgaagaac ttatcaacac ggctgaaatc
      241 aacatgaggt aagccatcaa tattgcgtat gcgtgttaat cgttctatat gttcctcacc
      301 ggggccagcg ccgccatcac cgacaccata caccataaac gcatagggac agatttcttt
      361 ttcattatag ttttgttcaa tatattctaa agagcgagcg cgggctcggc tatcatacgt
      421 atcttcagga aagttatgca tcagaatttc tgagccatcc aggccttgcc agcggaaaag
      481 gtaattatta aattta
//
LOCUS       DAZGLJ010000671          494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.684, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000671 DAZGLJ010000000
VERSION     DAZGLJ010000671.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.684"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..231
                     /locus_tag="ACHK49_005522"
     CDS             <1..231
                     /locus_tag="ACHK49_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265934.1"
                     /translation="ISADIILTRTPHITEFSSDSITAQIKKNGINIQTGRMVSQKHLI
                     ITHDQEGKHLTTWYQQISLRVHSYKKHTEEVR"
BASE COUNT          188 a          116 c           70 g          120 t
ORIGIN      
        1 atctcagcag atattatcct gaccaggaca cctcacatta ctgaattctc ttccgacagt
       61 atcactgcac aaataaaaaa gaacgggata aatattcaga ccggaagaat ggtgtcacaa
      121 aagcatttaa tcattacgca tgaccaggaa gggaaacact taaccacatg gtatcaacag
      181 atatcattac gtgtgcactc ttataaaaaa cacacagaag aagtaagata attataaacg
      241 taaatttaat atctcccact gacatctccc ccacaataca ctaaaaaata ctcacagcag
      301 taaacacaga cacacctgat aatcatacag ccgataaata ttttcccctt tacggagaaa
      361 tcagcctgct gtttaacaat aacggcgtat tttcatggaa ggagatcacc cagtaacgga
      421 taagtcctga tctccttcat gttaatacgc aacaatccca tcgattcaaa acaatttcag
      481 acagatatta tctc
//
LOCUS       DAZGLJ010000672          492 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.685, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000672 DAZGLJ010000000
VERSION     DAZGLJ010000672.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.685"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            59..>492
                     /locus_tag="ACHK49_005523"
     CDS             59..>492
                     /locus_tag="ACHK49_005523"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265935.1"
                     /translation="MNMIRSQSTESIDEFYRVIDELKICSSDKIFTDIINRISITRSD
                     IDDILEGVEKSTLDPSIPSLFRHCVEWGKLYPNGFYIKHDDSKAITEKKDIFDKFMDI
                     SKITEAYGYDRRKFELPIKARSLTFHSSQEYPQLQIVDIVASA"
BASE COUNT          176 a           65 c           96 g          155 t
ORIGIN      
        1 tttctgtttc cctgctttct gtggggagga aaaaacagag gtaatgtatc agtgttttat
       61 gaatatgatc agaagccaat cgactgagtc aattgatgaa ttttacaggg taattgatga
      121 gttaaagata tgctcaagtg ataagatatt cacagatata ataaacagaa ttagtattac
      181 cagaagcgat attgatgata tattggaagg tgttgagaaa agcacattag atccatctat
      241 accaagcctc tttagacact gtgtagaatg gggtaaatta taccctaatg gtttttatat
      301 taagcatgac gattccaaag ctattacaga gaagaaagat atttttgata aatttatgga
      361 tatatcaaaa attacggagg catatggtta tgatcgaaga aaatttgaat tgccaattaa
      421 agctcgttct ttgacattcc attcatcaca agagtatcct caattacaaa ttgtagatat
      481 agtagcaagt gc
//
LOCUS       DAZGLJ010000673          491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.686, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000673 DAZGLJ010000000
VERSION     DAZGLJ010000673.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.686"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..104)
                     /locus_tag="ACHK49_005524"
     CDS             complement(<1..104)
                     /locus_tag="ACHK49_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265936.1"
                     /translation="MFACTFFFLHGAGCLPVNSVSAPESAIITYTWLL"
     gene            344..>491
                     /locus_tag="ACHK49_005525"
     CDS             344..>491
                     /locus_tag="ACHK49_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO9265937.1"
                     /translation="MTFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAITA"
BASE COUNT          122 a          113 c          130 g          126 t
ORIGIN      
        1 atcagcaacc aggtatatgt gataatcgcg gattcaggtg ctgatactga attcaccggg
       61 aggcacccgg caccatgcaa gaaaaagaat gtgcatgcaa acatgcccct ctccggaggg
      121 gcttttttat gggtaaaaaa tgcccgaatg ggttcgggca atagcatgag atactgatat
      181 tgttgtgttg ttatcgtgtg gattttaacc agggtttatc aggctgcgca actgcgtggc
      241 cttttttcat ttcttgggct gtagtccccg tgtgtcattc aggcttccgg actacagccc
      301 actccatatc tgatttaata cactatcccg gccgggagga ataatgacat ttaaacatta
      361 tgatgttgtc agggcggcgt cgccgtcaga ccttgcggaa aagctgacac acaaactgaa
      421 agagggctgg cagccgtttg ggagtccggt ggccataacc ccttataccc tgatgcaggc
      481 gattacagca g
//
LOCUS       DAZGLJ010000674          490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.687, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000674 DAZGLJ010000000
VERSION     DAZGLJ010000674.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.687"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..386
                     /locus_tag="ACHK49_005526"
     CDS             <1..386
                     /locus_tag="ACHK49_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HFO9265938.1"
                     /translation="TRLNEAKCLLDHGFFHGAYYLCGYAVECALKACIAKSFLQHEFP
                     NKKVVNDSYTHDLSQLLKIANLHQILIADAKNDVSLEINWSVVKDWSEQFRYDNNISK
                     AMAEQLFDAVGDQNSGVLKWVKAHW"
BASE COUNT          157 a           75 c          102 g          156 t
ORIGIN      
        1 acactaggct taatgaggcc aaatgcttgc ttgatcatgg cttttttcat ggtgcatatt
       61 atctttgtgg gtatgcagtt gaatgtgcat tgaaggcctg tattgccaag tcatttttac
      121 aacatgagtt tccaaacaaa aaagtcgtaa atgattcata tactcatgat ttgtcgcaac
      181 ttctcaaaat tgccaactta catcaaattt tgattgctga cgcaaaaaat gatgtttcgt
      241 tggagattaa ctggtcggtc gttaaagact ggagcgagca atttagatac gacaataaca
      301 taagtaaagc tatggccgaa caattgtttg atgctgtagg tgaccaaaat tctggagttt
      361 tgaaatgggt aaaagcacac tggtaatcgg cagagagttg acaaaagata tggaattctc
      421 aggtcaattt ttgttaaaaa aactcaagtt acagaattta actattgatg ctgcaatgtg
      481 gttttattac
//
LOCUS       DAZGLJ010000675          487 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.688, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000675 DAZGLJ010000000
VERSION     DAZGLJ010000675.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.688"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT          187 a           92 c           81 g          127 t
ORIGIN      
        1 cttcttcctc cgtaatggtt ttctgaattt ggccacctga acagagctca ccaaagctat
       61 ggatgtcggt atttccacaa taccaagatg gcgaaaataa ctgcatgata agcctcaggg
      121 aaaagggaag acactacccc cgataattca gaaacaaatc gaaatacatg aactaaagaa
      181 aaaaccacaa caaacagaaa tagaaataga aatagaaata gaaatagaaa tagaaatatt
      241 aaaacagacc accacattcc tgatgtcata ctcactaaac aatttttcga caacatggta
      301 gctcatagaa cgttatcgtg tagacaccct ctgctacgta ttcggtgttc agtgcaatat
      361 ctacaaatac tggaaaaatc taaaatcgag gcaagttttt ggtgataact atagttagac
      421 tatattgacg acctgatgtg ctgtatgtaa taactaacaa aaaatatttt ccatgggatt
      481 ttttatt
//
LOCUS       DAZGLJ010000676          485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.689, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000676 DAZGLJ010000000
VERSION     DAZGLJ010000676.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.689"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>485
                     /locus_tag="ACHK49_005527"
     CDS             <1..>485
                     /locus_tag="ACHK49_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO9265939.1"
                     /translation="LVGKSAFVEASGQASNDAEGYLDFFRGEIGKTHLAQGLWELIDN
                     SQLADEMAEMKTSITETRNEITQTVNKTLEDQSATIQQIQRVQKDTNDDLAALYMLKV
                     QKTKDGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPENGNSTPLFVAQGNQLFM
                     N"
BASE COUNT          143 a          107 c          149 g           86 t
ORIGIN      
        1 ctggtgggca agtctgcgtt tgtggaagcc agcgggcagg ccagcaatga tgcagaaggg
       61 tatctggact ttttcagagg agaaatagga aaaacgcatc tggctcaggg gttgtgggaa
      121 ctgatagata acagccagct tgcagatgag atggcggaga tgaagaccag catcacggaa
      181 acccgcaatg aaatcacaca gacggtcaac aaaacgctgg aggaccagag cgccaccata
      241 cagcagatac agcgcgtgca gaaggacaca aatgatgacc tggctgcgct gtacatgctg
      301 aaggttcaaa aaacgaaaga cggcattccc tatgtggccg ggattggtgc agggattgag
      361 gatactgatg gccagccact gagcaacata ctgctgctgg ctgaccgtat cgcgatgata
      421 aatccggaga acggcaacag cacgccgtta tttgtggcgc aggggaatca gctgttcatg
      481 aacga
//
LOCUS       DAZGLJ010000677          476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.690, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000677 DAZGLJ010000000
VERSION     DAZGLJ010000677.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.690"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>476
                     /locus_tag="ACHK49_005528"
     CDS             <1..>476
                     /locus_tag="ACHK49_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HFO9265940.1"
                     /translation="LSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGAS
                     FEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGQD
                     SRLVTTAITADTEHRFSGLPPGEYTLTVRAINSYGQQGEPATTTFRINAPAVPATI"
BASE COUNT           96 a          147 c          152 g           81 t
ORIGIN      
        1 ctctccctgc cgtcactgcg tcgtcgcctg ttccgctgtg tttccatccg ggaaaacacg
       61 gacggtacct ttgccatcac ggcagtgcag catgtgccgg agaaagaagc catcgtggat
      121 aacggggcca gctttgagcc gcagtcaggc accctgaaca gcgtcatccc tccggcagtg
      181 cagcacctga cggtggaggt gagcgcagct gacggccagt atctggcgca ggcgaaatgg
      241 gacacgccgc gggtggtgaa gggcgtgcgc ttcagtctgc gcctgaccag tggaagcggt
      301 caggacagcc gtctggtgac caccgccatc accgcggata cagagcaccg tttcagcggt
      361 ctgccgccgg gggaatacac cctgacggtc agggcgatta acagttatgg ccagcagggg
      421 gaaccggcca ccaccacgtt caggattaat gcacctgcgg tacccgccac gattga
//
LOCUS       DAZGLJ010000678          476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.691, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000678 DAZGLJ010000000
VERSION     DAZGLJ010000678.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.691"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>476
                     /locus_tag="ACHK49_005529"
     CDS             <1..>476
                     /locus_tag="ACHK49_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HFO9265941.1"
                     /translation="LAALENEMIIFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIR
                     SRIEKIQKDESFTNRLEPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAW
                     IDFGYCRKPNVTRGLKIWDFPFDESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYII"
BASE COUNT          161 a           79 c           92 g          144 t
ORIGIN      
        1 tctggccgca cttgaaaatg agatgattat tttcacttct cccgatttga aaccaagggt
       61 tgaagcgatt cgcaacggaa aaccaacaac ggttatcgtt atcgatataa aaaagaaatt
      121 tagatatatc agaagtcgta tcgaaaaaat tcaaaaagat gagtcgttta caaatagact
      181 tgagcctcga caattaaaaa acccagagta ctggtcacca gagtatgttt tggtatgtaa
      241 ccttaaggcc tattttgtaa ataaagctat caacatgggc ttagtgaaaa cgcctcttgt
      301 tgcgtggata gattttggct attgccgtaa gccaaatgta actcgaggat tgaaaatttg
      361 ggatttccca tttgatgaaa gtaagatgca tcttttcact ataaaaaaag ggcttactgt
      421 cacatctcaa caacaggttt ttgatttcat gattggcaat catgtatata ttattg
//
LOCUS       DAZGLJ010000679          476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.692, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000679 DAZGLJ010000000
VERSION     DAZGLJ010000679.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.692"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>476
                     /locus_tag="ACHK49_005530"
     rRNA            <1..>476
                     /locus_tag="ACHK49_005530"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZGLJ010000680          473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.693, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000680 DAZGLJ010000000
VERSION     DAZGLJ010000680.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.693"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>473
                     /locus_tag="ACHK49_005531"
     CDS             <1..>473
                     /locus_tag="ACHK49_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc5 family transposase"
                     /protein_id="HFO9265942.1"
                     /translation="ARVVSCISPAKFHECFINWMRDCHSSDDKDVIAIDGKTLRHSYD
                     KSRRRGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLNMMDIKGKIITTDAMGC
                     QKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAMSEKSHG"
BASE COUNT          160 a           88 c          112 g          113 t
ORIGIN      
        1 gccagagttg tatcctgtat cagtcctgca aaatttcacg agtgctttat taactggatg
       61 cgtgactgcc attcttcaga tgataaagac gtcattgcaa ttgatggaaa aacgctccgg
      121 cactcttatg acaagagtcg tcgcagggga gcgattcatg tcattagtgc gttctcaaca
      181 atgcatagtc tggtcctcgg acagatcaag acggatgaga aatccaatga gattacagcc
      241 attcctgaac ttcttaacat gatggatatt aaaggaaaaa tcatcacaac tgatgcgatg
      301 ggctgccaga aagatattgc agaaaagata caaaaacagg gaggtgatta tttattcgcg
      361 gtaaaaggaa accaggggcg gctaaataaa gcctttgagg aaaaatttcc gctgaaagaa
      421 ttaaataatc cagagcatga cagttacgca atgagtgaaa agagtcacgg cag
//
LOCUS       DAZGLJ010000681          471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.694, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000681 DAZGLJ010000000
VERSION     DAZGLJ010000681.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.694"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..348)
                     /locus_tag="ACHK49_005532"
     CDS             complement(<1..348)
                     /locus_tag="ACHK49_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG"
                     /protein_id="HFO9265943.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEA"
BASE COUNT          149 a          124 c           81 g          117 t
ORIGIN      
        1 agcctcaagc gggcactgaa gtctctctgg acaaacagaa aacttacatt gagatatttt
       61 gttctgaata ctctcttgcg attgtgcttc acctgagtcc gaaagcatac ttagcattct
      121 ctcaccaagg gctcgaggac cactattaaa tcccaaaagc aataactcag aaagcaatcc
      181 gccattaccg ccactggatg aaaagcgccc attagtagaa tcatacacaa tactgacagt
      241 ttcaccaccg actgtaatat ctccgcgccc attggctaca attcgggcac gaacaacctc
      301 caccccaaga ttaaatgaat gtgatctaat atctgaggtt aatggcataa caaagctccc
      361 tattttaatt tgaactccag acttaaatag ctgtaacaaa catcccccat acatgacaac
      421 aaaaaccgga gccggactcc ggtttttgtg aagctgtcgg ctatttcatt c
//
LOCUS       DAZGLJ010000682          468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.695, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000682 DAZGLJ010000000
VERSION     DAZGLJ010000682.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.695"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(177..404)
                     /locus_tag="ACHK49_005533"
     CDS             complement(177..404)
                     /locus_tag="ACHK49_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO9265944.1"
                     /translation="MSSKNRPRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIES
                     CRRELAADIKYARQLTIKKNDTQYALRWLFI"
BASE COUNT          121 a           79 c          101 g          167 t
ORIGIN      
        1 ggaatctgtt caataaaaaa actgccgtgt tggagtcaca gcagtaatgt actgattggg
       61 tagaagatta ttattgttat gctttatttt tattctatat ggctgattat ttcaattcgg
      121 aattaataca gctaatgtct gtgatttttt ataaattcag caatataaag aaatagttat
      181 atgaacagcc atcgcagagc atactgtgta tcattctttt ttatagtcaa ctgacgggca
      241 tattttatgt ctgctgccag ctcccggcgg caagattcaa tgacccacgc agaaaaattt
      301 tctgaacctt tctggtcaag agcgatgtta atttgttcaa tcatctggtt tggaaatcgg
      361 atgttgcggg ttgttgttct gcggggccgg tttttcgatg acattttctt tcctctggtg
      421 acaagctata tggcgaggat tttacatggc tgtgcttcgt acgttacc
//
LOCUS       DAZGLJ010000683          467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.696, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000683 DAZGLJ010000000
VERSION     DAZGLJ010000683.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.696"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            106..>467
                     /locus_tag="ACHK49_005534"
     CDS             106..>467
                     /locus_tag="ACHK49_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HFO9265945.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVL"
BASE COUNT          133 a          117 c          105 g          112 t
ORIGIN      
        1 caaccaaatt ctccccacat caacctcatt gtgtgggtat aattgcgggc ataccccagt
       61 tcgacagaat ttgtaccctc tttaacgcat tcagaaggct gaactatggc actgactgac
      121 gcaaaaatcc gggctgcaaa gcccactgac aaggcttata aactcactga cggggctggc
      181 atgttcctgc tggtacatcc taatggttcc cgttactggc gtctccgtta tcgtattctg
      241 ggtaaagaga agactctggc acttggtgtg tatccagaag tatctctctc cgaagctcgt
      301 acaaaacggg atgaggcccg aaaactgatt tcggaggggg ttgacccttg cgaacagaaa
      361 agagctaaaa aagtagtccc tgatttacaa ctctctttcg aacatattgc acgacgctgg
      421 catgccagta ataaacaatg ggcacaatca cacagcgata aagtact
//
LOCUS       DAZGLJ010000684          459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.697, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000684 DAZGLJ010000000
VERSION     DAZGLJ010000684.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.697"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>459
                     /gene="espX1"
                     /locus_tag="ACHK49_005535"
     CDS             <1..>459
                     /gene="espX1"
                     /locus_tag="ACHK49_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO9265946.1"
                     /translation="CKLIEHTVQFYFFKHKGITLLRLDVFDDRTSECLSEEIKIYQEC
                     HEKFVNFLKANFNQEIYPQLYTPEIFYEACRNLQSFYDHQETSQNAKYSAIDKKKSHL
                     NQEIKKLIKKNIFPELYNEQCNKIPSSSIDDNQGIMWQNFKTSNIAYSQL"
BASE COUNT          186 a           81 c           65 g          127 t
ORIGIN      
        1 atgtaaactc atagaacata cagttcaatt ttattttttt aaacacaaag gtatcacatt
       61 acttagattg gatgtttttg atgacagaac aagcgaatgt ctttctgaag agattaagat
      121 ataccaagaa tgccatgaaa aattcgttaa ttttttaaag gcaaacttca accaagaaat
      181 atacccacag ctatatactc ctgaaatatt ttatgaggcc tgcagaaatc tccagtcatt
      241 ttatgatcat caggaaacgt cacaaaacgc aaaatacagt gcaatagaca agaaaaaaag
      301 tcatcttaac caggaaatca aaaagcttat taaaaaaaat attttccctg agctatacaa
      361 tgagcaatgc aataaaatcc cctcctcatc gatagatgat aaccagggaa taatgtggca
      421 gaactttaaa actagcaata ttgcatattc acaactatg
//
LOCUS       DAZGLJ010000685          458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.698, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000685 DAZGLJ010000000
VERSION     DAZGLJ010000685.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.698"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(1..398)
                     /locus_tag="ACHK49_005536"
                     /pseudo
     CDS             complement(1..398)
                     /locus_tag="ACHK49_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131658.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
BASE COUNT          140 a          113 c           76 g          129 t
ORIGIN      
        1 tttgacaaac actccttctt cgggtctctc tagtgtaatt ggacactgaa tagactctgg
       61 cgggcactgg aaatttcctg aactaacagg aaacttaaat tgagatattt tgtcctgaat
      121 actctcttgc gattgtgctt cacctgagtc cgaaagcata ctgagcatcc tatcgcgaag
      181 agcttcaggg ccgttattaa acccataaag gaataactca gtaagcaatc ccccactact
      241 gccgctggat agaaaacgcc catcggtttc attaaataca atgctgacag tctcattacc
      301 taatgtaaac tcccctcgac cgctagatgc tacttgagct cgaaggacat tcaatccatt
      361 aaacatgagc ttgatctaat ctccgaattt aacggcatta taaaactccc tattttaatt
      421 taaactccag acttaaatag ctttaacaaa catctgcc
//
LOCUS       DAZGLJ010000686          457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.699, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000686 DAZGLJ010000000
VERSION     DAZGLJ010000686.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.699"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            173..>457
                     /locus_tag="ACHK49_005537"
     CDS             173..>457
                     /locus_tag="ACHK49_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO9265947.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDELQRQVKGVVTWFELGENDKNQMLYSMKV"
BASE COUNT          107 a          114 c          102 g          134 t
ORIGIN      
        1 ttattttttt gactgaacag acacccgttt ctgtaaagta atatttaaac aaaattctca
       61 cataaccata gcattttctg ttccttccct aataacgtct gaagtcgtcc cgcttatcac
      121 attatccgca gttgtacggt acggatcatt attcatttta ccggaggttg ctatgtcaac
      181 cggattacgt ttcacactgg aagtggacgg cctgccgccg gacgcttttg cggtggtttc
      241 ctttcatctg aaccagtcac tctcttcgct cttttccctc gatctctccc tggtcagcca
      301 gcagtttctc tcccttgaat ttgcgcaggt gctggacaaa atggcctacc tgacgatatg
      361 gcagggcgat gaattacagc gccaggtgaa aggcgtggtg acctggtttg aactcgggga
      421 gaacgacaaa aaccagatgc tgtacagcat gaaggtg
//
LOCUS       DAZGLJ010000687          453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.700, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000687 DAZGLJ010000000
VERSION     DAZGLJ010000687.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.700"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(83..>453)
                     /locus_tag="ACHK49_005538"
     CDS             complement(83..>453)
                     /locus_tag="ACHK49_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265948.1"
                     /translation="CEALNRHVRVIVFSFNKLITPDPRVELYYRFNHEEEYYPSHRFM
                     LVVDMKTVMVFSQRAESLGLYSNNRLLINIIAEHIHSDIYLTKYQQQYPDANALINTL
                     HESGNDMVQDEKHLSDSKNR"
BASE COUNT          158 a           76 c           81 g          138 t
ORIGIN      
        1 acctttgcat aaaaaagttt agacgttata aaagacaaat agattgtgat cgcctacaga
       61 cgatcacaat gaaaggaaaa tattaacggt ttttgctatc actcaaatgt ttttcatcct
      121 gcaccatatc attgccagat tcatgcaaag tatttattaa tgcattggca tctggatatt
      181 gttgctgata cttggttaga tatatatcac tatggatatg ttcggcaatt atattgataa
      241 gcaagcgatt attcgagtat aaccccagtg attcagctcg ttgtgaaaaa accattactg
      301 tcttcatatc aacaacaagc ataaaacggt gagaagggta atattcttct tcatggttaa
      361 agcggtaata cagttctaca cgtgggtcgg gagtaataag tttattaaat gaaaaaacaa
      421 taactcgaac atgtcgattc agagcctcac aaa
//
LOCUS       DAZGLJ010000688          449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.701, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000688 DAZGLJ010000000
VERSION     DAZGLJ010000688.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.701"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..351)
                     /gene="espM1"
                     /locus_tag="ACHK49_005539"
     CDS             complement(<1..351)
                     /gene="espM1"
                     /locus_tag="ACHK49_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HFO9265949.1"
                     /translation="MPVNATGVSFSSFGISYHKDNSFRGTIRGKNDEVVKCSMGERSI
                     RFNVNKFSGCILETVSRQSTKDIHGWVSDERTVYPSRVINQEIDNCCLQKNAKISSEE
                     RKMVFSLVSKEFELT"
BASE COUNT          143 a           98 c           62 g          146 t
ORIGIN      
        1 agttagttca aactccttac ttacaagaga aaagaccatt ttcctctctt cagaagaaat
       61 ttttgcattt ttttgaaggc agcaattatc aatctcttgg ttgataaccc ttgacggata
      121 tactgtccgt tcatcactta cccatccatg aatatcttta gtactctgcc tgcttaccgt
      181 ctccagtata cagccgctaa atttgttaac attaaagcgg atagagcgct ctcccattga
      241 acacttcacg acctcatcat tcttcccacg gatggtgccc cggaaagaat tatctttatg
      301 ataactgata ccaaaagagc tgaaggacac acctgtcgca tttactggca taacaaatcc
      361 ttttcattaa aatttgctta caaaaattgt atataatcat tttaattaaa aaaatacatg
      421 tgtaaatagc attcgttcaa aatttaatt
//
LOCUS       DAZGLJ010000689          447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.702, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000689 DAZGLJ010000000
VERSION     DAZGLJ010000689.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.702"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..384
                     /locus_tag="ACHK49_005540"
     CDS             <1..384
                     /locus_tag="ACHK49_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9265950.1"
                     /translation="TVRNPNARIIVNRGDLPAIKLGIRMLGHRPNSILKAGQHRYQRA
                     FIQRLNNGRWHVMQRLPEARYAKGNDDKGRKKRNRLPIQVVKIPMAAPLKQAFDENVN
                     RIRRERLPKELGYALKQQLRIVIKR"
     gene            381..>447
                     /locus_tag="ACHK49_005541"
     CDS             381..>447
                     /locus_tag="ACHK49_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9265951.1"
                     /translation="MKHTDIRAAVLDALEQHEHGAT"
BASE COUNT          128 a          101 c          121 g           97 t
ORIGIN      
        1 acggtcagaa atccgaatgc cagaattatc gttaaccgcg gtgatctccc tgctattaag
       61 ctggggatca ggatgcttgg tcatcgtccg aacagcatac ttaaagccgg tcagcatcgt
      121 tatcagcggg cattcatcca gcgattaaat aatgggcgct ggcatgttat gcaacgtttg
      181 ccagaagccc ggtatgcgaa gggcaatgac gataaaggaa ggaaaaagcg taatcgtctt
      241 cccattcagg tggttaaaat tccgatggcg gccccactga agcaggcttt tgatgagaac
      301 gttaaccgta tccggcgaga acgtctgcca aaagaactgg gctatgcgtt gaaacaacaa
      361 ctaaggattg tgataaagcg atgaaacata ctgatatccg tgctgcagtg ctggatgcac
      421 tcgagcagca tgaacacggg gcgacgc
//
LOCUS       DAZGLJ010000690          445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.703, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000690 DAZGLJ010000000
VERSION     DAZGLJ010000690.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.703"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            50..>445
                     /gene="espW"
                     /locus_tag="ACHK49_005542"
     CDS             50..>445
                     /gene="espW"
                     /locus_tag="ACHK49_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFO9265952.1"
                     /translation="MPKISSVVSSCYHLFSEHQQLSNETTMTNSVSRRIVHKEYGISL
                     KSVPVWLATAKTPLALLNGRHTRSHSFIIAGTPGMGSRSGAQYYAINSDDKRSRIDID
                     SLFLKKLNNARNQNKFPIDVKETVIKLQGQ"
BASE COUNT          167 a           89 c           71 g          118 t
ORIGIN      
        1 tacaataagt taatcaccat ataacaaaca tatagcgagg agattattta tgcccaaaat
       61 atcatcagtt gtatcatcat gttaccatct gttcagtgaa catcaacaac tttcaaatga
      121 aacaacaatg acgaactccg tctccagaag aattgttcat aaagaatatg gtatatcttt
      181 aaaatccgtt cctgtatggt tggctacagc taaaactccc cttgctctac tcaatggcag
      241 acatacaaga agtcactcat ttattattgc agggactcca ggaatgggaa gcaggagcgg
      301 agcccaatac tatgccataa acagtgatga taaacgctcc cgcatagaca ttgactcttt
      361 atttttaaaa aagttaaata atgcgcgaaa tcaaaataaa tttccaatcg atgtaaaaga
      421 aacggttata aaactacaag gccaa
//
LOCUS       DAZGLJ010000691          443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.704, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000691 DAZGLJ010000000
VERSION     DAZGLJ010000691.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.704"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>443)
                     /locus_tag="ACHK49_005543"
     CDS             complement(<1..>443)
                     /locus_tag="ACHK49_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO9265953.1"
                     /translation="SARWMSKSEKNNKQKEAIEKFKKEVSHMNGGQQFIWGFDKVIQE
                     GLSGLIELSIDINDSTNHRDKSSLSPDGRAVLHFLGTIWNMAMGAVPGYNALSGVSSI
                     LHSAIVKESSNICDYIQGAVRIGMDFVPGTRSDLHSRSLQIKYEA"
BASE COUNT          137 a          106 c           62 g          138 t
ORIGIN      
        1 agcctcatat tttatctgca gcgaacggct atgtaagtca gagcgagtgc ctggaacaaa
       61 gtccattcca atacgtacag ccccctgaat ataatcacag atattgctag attcttttac
      121 tatagcacta tgtaaaatgc tacttacacc agacaatgca ttataaccag gtacagctcc
      181 catcgccata ttccaaattg tacctaaaaa atgtaacaca gctctcccat caggagaaag
      241 agaagactta tcacgatgat ttgtactatc attaatatcg atacttaact caatcaatcc
      301 acttaagcct tcttgaataa ccttatcaaa cccccaaata aactgctgcc cgccattcat
      361 atgggatact tcttttttga acttttctat tgcctctttt tgtttattat tcttttctga
      421 ttttgacatc cagcgagcag atg
//
LOCUS       DAZGLJ010000692          443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.705, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000692 DAZGLJ010000000
VERSION     DAZGLJ010000692.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.705"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(23..205)
                     /locus_tag="ACHK49_005544"
     CDS             complement(23..205)
                     /locus_tag="ACHK49_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265954.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNTEDN
                     DEAASDYALKINENSD"
BASE COUNT          143 a           96 c           67 g          137 t
ORIGIN      
        1 cttattatca gctacccccg tattaatcag agttttcatt tatttttaat gcataatcag
       61 atgccgcctc atcattatcc tcagtattat catagccgaa aacttctccc ggacacgggc
      121 gttcacgata atagtcatcg tcatagtttt catcgtcaga taatgatatg tcgtattctg
      181 aatatgattc accacaatta tacatgaaat catctccttt ggggggggca ataattagtg
      241 agtttattcg aaaaaatgat gagcgccaga ccaatccacc caacttttga ttataatcat
      301 gaagatactt gtctcagtac aaattgaatc aaactcaatg cagcatgatt cacctttata
      361 ttctctcagg gaaaccaaat accactactc aactattaag aacaaaattc gggatgtatg
      421 attatcagaa gatacctgca tcc
//
LOCUS       DAZGLJ010000693          440 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.706, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000693 DAZGLJ010000000
VERSION     DAZGLJ010000693.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.706"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..108
                     /locus_tag="ACHK49_005545"
     CDS             <1..108
                     /locus_tag="ACHK49_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265955.1"
                     /translation="EDAIKMRKYEPYLLNDNSILSRIALLELGIIGEQQ"
     gene            105..293
                     /locus_tag="ACHK49_005546"
     CDS             105..293
                     /locus_tag="ACHK49_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO9265956.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYDSKSSCIQVRDEQKIPGE
                     CLPLKKVSLNLNNEIPAG"
BASE COUNT          146 a           94 c           90 g          110 t
ORIGIN      
        1 gaagatgcca ttaagatgag aaaatatgaa ccttatcttc tcaatgataa ttccatactt
       61 tccagaattg cccttcttga acttggtatt atcggagaac agcagtgact tcagcatttg
      121 cactggtgat gaccgttttt cttataacgg gtgagccaca aaatgtgatt accggaattt
      181 atgacagtaa gtcatcctgc attcaggtaa gggacgaaca aaaaatcccc ggtgaatgcc
      241 tcccgttaaa aaaagtatcg ctgaacctga ataacgaaat accggctgga taacccgcca
      301 gccatattaa cgccatacca acggattaaa aatgccagca atggcaggga ttcgttcacc
      361 ctgaaatctg taatgaggtt aaaacaaaat gagtaaagtc tttatttgcg ccgccattcc
      421 ggacgaacag gcaataaagg
//
LOCUS       DAZGLJ010000694          430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.707, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000694 DAZGLJ010000000
VERSION     DAZGLJ010000694.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.707"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>430
                     /locus_tag="ACHK49_005547"
     CDS             <1..>430
                     /locus_tag="ACHK49_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265957.1"
                     /translation="NGDLNNVITIGIGFDPEQTIGVEYKTYLEELGDIFDIHFIKMKD
                     IADETEKVYVEFKIAWSQLHRAAYIKNYAVSINNEFIGELSNDEYTKSSLVNRLNFTH
                     PLLLPIDYNLWLESIDKEGLDTIASEFEISLNDLIPGLERN"
BASE COUNT          155 a           50 c           77 g          148 t
ORIGIN      
        1 taatggggat ctgaataatg taattactat tggtatcggt ttcgatcctg aacaaacaat
       61 aggtgtagaa tataaaacat atttagaaga acttggtgat attttcgata ttcattttat
      121 aaaaatgaaa gacattgcag atgaaactga aaaggtttat gtagagttta aaatcgcatg
      181 gtcacaattg cacagagcag catatataaa gaactatgct gtttctataa ataatgaatt
      241 tattggagag ctaagtaatg atgagtatac taaatcatct ttagtaaata gattgaactt
      301 tactcatccg ttgcttcttc ctattgatta taatttatgg cttgaatcta ttgataagga
      361 aggattagat acgattgcca gtgaatttga aatatcttta aatgacttaa ttcctggctt
      421 agaacgtaat
//
LOCUS       DAZGLJ010000695          429 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.708, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000695 DAZGLJ010000000
VERSION     DAZGLJ010000695.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.708"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>429
                     /locus_tag="ACHK49_005548"
     CDS             <1..>429
                     /locus_tag="ACHK49_005548"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFO9265958.1"
                     /translation="MPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIASNVKIMGGNHPL
                     NRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWIGNDVVLKGGIAI
                     GDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVID"
BASE COUNT          127 a           68 c           92 g          142 t
ORIGIN      
        1 aatgcctgtg aaaaatttct ccagtgtcgg cgctttttca tttccgacct gccatttttc
       61 agggaatata cgtataggtc gattttgtag tattgcatca aatgtaaaaa taatgggtgg
      121 aaatcatcct ttgaatcgtt ttactactca catgatgact tataacggtg aatttgataa
      181 gtttgctatg agtgaatttg aacgtagctg gacattgaaa ccattcatta ccaaacctga
      241 aaatccgatt ataggaaatg atgtctggat aggaaatgat gttgtattga aaggtggcat
      301 cgcaataggt gatggtgctg ttattgctgc caattccgtt gttaccaaag atgtgccccc
      361 atatgccata gttgcaggag ttcctgcaaa aataatcaga tttaggtttg attcaaatgt
      421 tattgatga
//
LOCUS       DAZGLJ010000696          428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.709, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000696 DAZGLJ010000000
VERSION     DAZGLJ010000696.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.709"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>428)
                     /locus_tag="ACHK49_005549"
     CDS             complement(<1..>428)
                     /locus_tag="ACHK49_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265959.1"
                     /translation="TDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLT
                     EKTDRIPTGVIRTDDERTHHYHYDSQHRLVFHTRIQHGEPLVESRYLYDPLGRRMAKR
                     VWRRERDLTGWMSLSRKPEETWYGWDGDRLTTVQTDTTRI"
BASE COUNT           53 a          142 c          133 g          100 t
ORIGIN      
        1 tggatacgcg tggtgtcggt ctgtaccgtc gtcagcctgt cgccgtccca gccgtaccat
       61 gtctcctccg gtttacgcga cagcgacatc caccccgtca ggtcacgctc ccgccgccat
      121 acccgtttcg ccattcgccg tcccagcggg tcgtagaggt agcggctctc gaccagtggc
      181 tcgccatgct gtatccgcgt gtggaacacc aggcggtgct ggctgtcgta gtggtagtgg
      241 tgggtccgct cgtcgtccgt ccgtatcaca cccgtcggga tgcggtccgt cttctccgtc
      301 agtctgccgt attcatcata gtggtaaaca tagtgcgcat cctctgcgat gcggttatcc
      361 ggccacgccg tgagcgtgct gtccgggtgt agctccgggt ccggcaggcg gtttcccgcc
      421 gggtcggt
//
LOCUS       DAZGLJ010000697          427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.710, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000697 DAZGLJ010000000
VERSION     DAZGLJ010000697.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.710"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..345
                     /locus_tag="ACHK49_005550"
     CDS             <1..345
                     /locus_tag="ACHK49_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265960.1"
                     /translation="EKESQRKMVSIRLPFISIYWLNREATNYWYTCARAAFNDPDWFV
                     KNHHAVRQAKRKANMTYMKAYQKAWKEHRDRYQQDMEKLESENMELRRKLGEAKRDID
                     AYKRLFNGESHA"
     gene            305..>427
                     /locus_tag="ACHK49_005551"
     CDS             305..>427
                     /locus_tag="ACHK49_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO9265961.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHV"
BASE COUNT          152 a           89 c           99 g           87 t
ORIGIN      
        1 gagaaagagt cacaacgaaa aatggtgtct atccggttgc catttatcag tatttattgg
       61 ctaaacaggg aggcaacaaa ttattggtat acatgcgcca gagcagcatt taacgaccct
      121 gactggtttg tgaaaaacca ccacgcagtt cgtcaggcaa agagaaaggc caatatgaca
      181 tacatgaagg cgtatcaaaa agcatggaaa gaacaccgcg atcgatacca acaagacatg
      241 gaaaagcttg aatcagaaaa catggaatta agacgaaagc tcggtgaagc aaaacgagac
      301 attgatgctt acaagcgact ttttaatggt gaaagccatg cttagcccat cccaatccct
      361 tcaataccag aaagaaagcg tcgagcgagc tttaacgtgc gctaactgcg gtcagaagct
      421 gcatgtg
//
LOCUS       DAZGLJ010000698          417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.711, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000698 DAZGLJ010000000
VERSION     DAZGLJ010000698.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.711"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..313)
                     /locus_tag="ACHK49_005552"
     CDS             complement(<1..313)
                     /locus_tag="ACHK49_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9265962.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQVYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VV"
     gene            complement(313..>417)
                     /locus_tag="ACHK49_005553"
     CDS             complement(313..>417)
                     /locus_tag="ACHK49_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9265963.1"
                     /translation="PYAWRQIKVTCAAWSSRVRMLRVEFSAEFKQVVN"
BASE COUNT           90 a          150 c           88 g           89 t
ORIGIN      
        1 ggaccaccgt ggcccccacc aggctctgca ggtcctccgc catcccggtg accagaccaa
       61 acagattgga caccgtcagc gacggtctgg cactgctgcc cttcccgttc atctcaaaac
      121 cgctgccgtc aatcgggtat acctgatatt gccgcccctg ccaggtgacc gcctcccctt
      181 tttcattcag ctcattacag aaaaaatacc gctcaccgcc ctgcaccgtc aggtcaattt
      241 cccagagcac cacccgcggt gactgctctg atttaaccga ctcgttcaga ctttcttcgt
      301 gaatatcctg catcagttca ccacctgctt aaactcggca ctgaattcaa cccgcagcat
      361 gcgaacccgt gatgaccagg cggcacaggt cacctttatc tgccgccagg cataagg
//
LOCUS       DAZGLJ010000699          411 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.712, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000699 DAZGLJ010000000
VERSION     DAZGLJ010000699.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.712"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(37..192)
                     /gene="yrhD"
                     /locus_tag="ACHK49_005554"
     CDS             complement(37..192)
                     /gene="yrhD"
                     /locus_tag="ACHK49_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrhD"
                     /protein_id="HFO9265964.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
BASE COUNT          134 a           64 c           62 g          151 t
ORIGIN      
        1 atttcttttt cagaacgtcg aacgggattt tcttatttaa gatatcagaa tcttatgatt
       61 cgggtaaaat tctaaataaa tcaacatgtc attaaaatca tcatagccat tagctatttc
      121 ggctaaaata gagactacat gtcttcggtc catctcactt aaggagtgta gttccgttgt
      181 aagtttttcc atagcttgca ctgctaaatt tcgaacaagg aattttctgc tggtaatctc
      241 taaaaagatg gcatggttta caatgatttt tgtttccttt tgattattat gaacaactgt
      301 ccatgatttc gtttaagaat gaagagaaat cactaaacga actgaatata ttttctgtgc
      361 caatattatc tctaatttca aaaaagttac ttttaatgtc ataagtaaat a
//
LOCUS       DAZGLJ010000700          411 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.713, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000700 DAZGLJ010000000
VERSION     DAZGLJ010000700.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.713"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..293)
                     /locus_tag="ACHK49_005555"
     CDS             complement(<1..293)
                     /locus_tag="ACHK49_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO9265965.1"
                     /translation="MAFLLNKSDMASSIGISVQAFDKWGVPPVERRGREVFYDVKTVL
                     EIDRERRQHNQRIPDDEGDLEERLLRARAELTEEQAVAQKLKNQVTEGKLIDTG"
BASE COUNT          109 a          109 c           72 g          121 t
ORIGIN      
        1 ccggtgtcaa taagcttacc ttcggttacc tgatttttaa gtttttgagc tacggcctgt
       61 tcttctgtca gttcagctct ggcccgaagc agcctttcct ccagatcgcc ctcgtcatca
      121 ggtattctct gattgtgttg tcgccgctcg cgatctatct ccagtacagt tttaacgtca
      181 taaaaaactt ccctcccccg acgctcaaca ggaggaacgc cccatttatc aaatgcctga
      241 acagatatac cgatggagga ggccatatca cttttattca ataaaaaggc catctcctct
      301 ccataagcca tcgataaaaa gcgatacaac aaccatgtgt ttttacaaaa ccatttgata
      361 tcattgacat ttttcgcatt gacgacatca aaacacatcg taaggttgtt g
//
LOCUS       DAZGLJ010000701          403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.714, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000701 DAZGLJ010000000
VERSION     DAZGLJ010000701.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.714"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            162..>403
                     /locus_tag="ACHK49_005556"
     CDS             162..>403
                     /locus_tag="ACHK49_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO9265966.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT          124 a           79 c           71 g          129 t
ORIGIN      
        1 tcatattgca ttgtttttca atgaaaaaat attattcgcg taatatctca cgataaataa
       61 cattaggatt ttgttattta aacacgagtc ctttgcactt gcttacttta tcgataaatc
      121 ctactttttt aatgcgatcc aatcatttta aggagtttaa aatggataag aagcaagtaa
      181 cggatttaag gtcggaacta ctcgattcac gttttggtgc gaagtctatt tccactatcg
      241 cagaatcaaa acgttttccg ctgcacgaaa tgcgcgacga tgtcgcattc cagattatca
      301 atgacgaatt atatcttgat ggcaacgctc gtcagaacct ggccactttc tgccagacct
      361 gggacgacga aaatgtccac aaattgatgg atttatccat taa
//
LOCUS       DAZGLJ010000702          402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.715, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000702 DAZGLJ010000000
VERSION     DAZGLJ010000702.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.715"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            12..>402
                     /locus_tag="ACHK49_005557"
     CDS             12..>402
                     /locus_tag="ACHK49_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0046903 - secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HrpJ domain-containing protein"
                     /protein_id="HFO9265967.1"
                     /translation="MANGIEFNQSTASVFNSNSLDFELESQQLTQKNSSNSYSPLLNL
                     QNELAMITSSSLSETIEGLSLGYRKGSARKEEEGTTTEKLLNDMQELLTLTDSDKLKE
                     LSLKNSGLLEQHDPNLAMFGNMPKGEIV"
BASE COUNT          138 a           61 c           85 g          118 t
ORIGIN      
        1 gtgagtttcc aatggctaat ggtattgaat ttaatcaaag caccgcatct gtttttaatt
       61 ctaattcatt agattttgaa ttggaatctc aacaattaac gcaaaaaaat tcttctaata
      121 gttattctcc attattaaat ttacaaaatg aattagcgat gattactagt tcatcgcttt
      181 ctgaaacgat tgaagggcta agcctgggat atcgcaaagg cagtgcaagg aaagaagagg
      241 aaggtacgac aacggagaag ctgcttaatg atatgcaaga gctgcttact ctgaccgata
      301 gcgataaatt aaaagaacta tcattaaaga acagtgggct gctggaacag catgatccta
      361 atttggctat gtttggtaat atgccaaaag gggagattgt tg
//
LOCUS       DAZGLJ010000703          399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.716, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000703 DAZGLJ010000000
VERSION     DAZGLJ010000703.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.716"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>399
                     /locus_tag="ACHK49_005558"
     rRNA            <1..>399
                     /locus_tag="ACHK49_005558"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          108 a           94 c          122 g           75 t
ORIGIN      
        1 ttagcggatg acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct
       61 ccccgaaagc tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc
      121 ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta
      181 tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg
      241 ccagctaagg tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc
      301 aggatgttgg cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt
      361 cggcctgcgc ggaagatgta acggggctaa accatgcac
//
LOCUS       DAZGLJ010000704          393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.717, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000704 DAZGLJ010000000
VERSION     DAZGLJ010000704.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.717"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>393
                     /locus_tag="ACHK49_005559"
     CDS             <1..>393
                     /locus_tag="ACHK49_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO9265968.1"
                     /translation="ALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQL
                     TDIVAGNKNELLLLDLSHNKLTSLHNDLFPNLNTLLINNNLLSEIKIFYSNFCNVQTL
                     NAANNQLKYINLDFLTYLPSIKSLRLDNN"
BASE COUNT          137 a           73 c           52 g          131 t
ORIGIN      
        1 gctctcagaa atattgatat agataggctt tcatcagtta cttattttag tgcggcacat
       61 aatcaactag agtttgtgca attagaatct tgcgaatggc tgcaatacct gaatctcagc
      121 cataatcaat taactgatat tgttgcaggt aataaaaatg aactcttact gctggatcta
      181 tcccataata aactaacaag tttacacaat gacttatttc ccaacttgaa tacgttactt
      241 attaacaaca atttgctttc tgaaattaaa atattctata gcaacttctg caatgttcag
      301 acattaaacg ctgctaacaa ccagttgaaa tatataaatc ttgatttcct gacttatctt
      361 ccatctatca aaagtttaag actggacaat aat
//
LOCUS       DAZGLJ010000705          391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.718, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000705 DAZGLJ010000000
VERSION     DAZGLJ010000705.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.718"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>391
                     /locus_tag="ACHK49_005560"
     CDS             <1..>391
                     /locus_tag="ACHK49_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="The RecE (also known as exonuclease VIII) pathway
                     is an alternative pathway for the initiation of homologous
                     recombination in Escherichia coli. The RecE pathway is
                     activated in recB, recC sbcA (suppressors of recB and
                     recC) mutants. Activation of the RecE pathway results in
                     the production of at least two phage proteins relevant for
                     recombinational repair. RecE is a duplex DNA-specific
                     exonuclease which produces 3' overhangs in duplex DNA by
                     preferentially degrading the 5'-ending strands.
                     Recombinational repair of E.coli chromosomal DNA involves
                     as well other proteins: RecA, RecF, RecJ, RecO, RecR, and
                     RuvC; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease VIII"
                     /protein_id="HFO9265969.1"
                     /translation="KGIQTRDCVYQNQPETEKTSPDMNQPEPVVQQEPEIACNACGQT
                     GGDNCPDCGAVMGDATYLETFDEESQVEAKENDPEEMEGAEHPHNENAGSDPHRDCSD
                     ETGEVADPVIVEDIEPGIYYGISNENYH"
BASE COUNT          123 a           86 c          114 g           68 t
ORIGIN      
        1 aaggggatcc agactcgcga ttgtgtgtac cagaaccagc cagaaacgga aaaaaccagc
       61 ccggatatga atcaacctga gccagtagtg caacaggaac cggaaatagc ctgcaatgcc
      121 tgcggccaga ctggcgggga taactgccct gactgtggtg cggtgatggg cgacgcaaca
      181 tacctggaaa cattcgatga agagagtcag gttgaagcta aggaaaatga tccggaggaa
      241 atggaaggcg ctgaacatcc gcacaatgag aatgctggca gcgatccgca tcgcgattgc
      301 agtgatgaaa ctggcgaagt cgcagatccc gtaatcgtag aagacataga gccaggtatt
      361 tattacggaa tttcgaatga gaattaccac g
//
LOCUS       DAZGLJ010000706          391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.719, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000706 DAZGLJ010000000
VERSION     DAZGLJ010000706.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.719"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            3..>391
                     /gene="nleE"
                     /locus_tag="ACHK49_005561"
     CDS             3..>391
                     /gene="nleE"
                     /locus_tag="ACHK49_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HFO9265970.1"
                     /translation="MNTKYAEHVVKNIYPEIKHDYFNESPNIYDKKYISGITRGVAEL
                     KQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTVISGKRPMGQFSVD
                     SLYNPDLHALCELPDICCKIFPKENND"
BASE COUNT          139 a           63 c           75 g          114 t
ORIGIN      
        1 ctataaatac taaatatgct gaacatgtgg tgaaaaatat ttacccggaa attaaacatg
       61 attactttaa tgaatcaccc aatatatatg ataagaagta tatatccggt ataaccagag
      121 gagtagctga actaaaacag gaagaatttg ttaacgagaa agccagacgg ttttcttata
      181 tgaagactat gtattctgta tgtccagaag cgtttgaacc tatttccaga aatgaagcca
      241 gtacaccgga aggaagctgg ctaacagtta tatccggaaa acgcccaatg gggcagtttt
      301 ctgtagatag tttatacaat cctgatttac atgcattatg tgagcttccg gacatttgtt
      361 gtaagatctt ccctaaagaa aataatgatt t
//
LOCUS       DAZGLJ010000707          389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.720, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000707 DAZGLJ010000000
VERSION     DAZGLJ010000707.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.720"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            89..>389
                     /gene="grlR"
                     /locus_tag="ACHK49_005562"
     CDS             89..>389
                     /gene="grlR"
                     /locus_tag="ACHK49_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE GrlA-binding
                     negative regulator GrlR"
                     /protein_id="HFO9265971.1"
                     /translation="MIMKDGIYSIIFISNEDSCGEGILIKNGNLITGGDIASVYQGVL
                     SEEEDIILHVHRYNHEIPSVLNIEQDYQLVIPKKVLSNDSNVTLHCHVRGNEKLFV"
BASE COUNT          131 a           51 c           88 g          119 t
ORIGIN      
        1 agaaaaagaa aggtctccgt tattcttgat atcgcttatg gatagaacaa attgaaagga
       61 gtgaggttag tatgaaactg agtgagttat gattatgaag gatggcatct atagcattat
      121 ctttattagc aatgaagatt cctgtgggga aggtatactg attaaaaatg gaaatttgat
      181 cactggcgga gatattgctt ctgtgtatca gggggtcctg tctgaagagg aggacatcat
      241 acttcatgtc catcgctata atcatgaaat tccctcggtg ctaaacattg aacaagatta
      301 tcaattagtt atccctaaaa aagtactgag taatgatagt aatgtcacat tacattgcca
      361 tgtaagagga aatgaaaaat tgtttgttg
//
LOCUS       DAZGLJ010000708          387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.721, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000708 DAZGLJ010000000
VERSION     DAZGLJ010000708.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.721"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..387
                     /locus_tag="ACHK49_005563"
                     /pseudo
     CDS             1..387
                     /locus_tag="ACHK49_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842356.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          136 a           52 c           66 g          133 t
ORIGIN      
        1 gataatttta tacccttgat aagcttgttg agctcagaac tagaaaaaaa agaataaaca
       61 tgataaacga tctgaagagt atattgttaa aatcctcaga ggaagttgat gtttttatca
      121 aaatttttga aagttggaga aaaaaattac cggcaatatc aggtcctgta aatctctata
      181 tacctaccag attcaaagat aagtatcttg aagtagagtc atattttgtc gacaaatcaa
      241 tatggaatgt ccatattaca tttcatgatg ataagcgttt tgtgtttttt accgatcaat
      301 ttattgctga atttagtccg caggaatttg tggataattg tgagcaatat ttaattaata
      361 atcattgttt ctcccctgac aaggtta
//
LOCUS       DAZGLJ010000709          384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.722, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000709 DAZGLJ010000000
VERSION     DAZGLJ010000709.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.722"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>384)
                     /locus_tag="ACHK49_005564"
     CDS             complement(<1..>384)
                     /locus_tag="ACHK49_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265972.1"
                     /translation="EYIQSIVSDKAVSYAAAQHHKKELLPEALLEDFSLASPYNVFET
                     LKDIIGMKGQRPIEMMKCSSEFMKVCELRHCCVHRFGKLGSKNAIRLGLAEHMKHLEK
                     PIILNNDDLEQIAFIVENFIRTLNN"
BASE COUNT          115 a           78 c           56 g          135 t
ORIGIN      
        1 tattattcaa agttctaata aaattctcaa caataaatgc gatttgctcg agatcatcat
       61 tattgagtat tatcggtttt tctaagtgct tcatatgttc agccaatcct agtcggattg
      121 catttttcga acccaactta ccaaatcgat gaacacaaca atgccttaac tcgcaaactt
      181 tcatgaattc gcttgagcat ttcatcattt cgattggtcg ttgtcctttc attccaataa
      241 tgtcctttaa tgtttcaaaa acattataag ggcttgccaa tgaaaagtcc tcaagtaaag
      301 cttctggtaa taactccttt ttatggtgtt gtgccgctgc ataactaacg gccttatcag
      361 aaactataga ctgtatatat tcat
//
LOCUS       DAZGLJ010000710          382 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.723, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000710 DAZGLJ010000000
VERSION     DAZGLJ010000710.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.723"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>382
                     /locus_tag="ACHK49_005565"
     CDS             <1..>382
                     /locus_tag="ACHK49_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO9265973.1"
                     /translation="DNLKSTQLLSVIDAISEGPIEGPVDGLKSVLLNSTPVLDSEGNT
                     NISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITRTITSANIDRLRFTFG
                     VQALVETTSKGDRNPSEVRLLVQI"
BASE COUNT           92 a           99 c          120 g           71 t
ORIGIN      
        1 aagataacct gaaatccacg cagctgctga gtgtgattga tgccatcagc gaagggccga
       61 ttgaaggtcc ggtggatgga ttaaaaagcg tgctgctgaa cagtacgccg gtgctggaca
      121 gtgaggggaa taccaacatc tccggcgtca cggtggtgtt ccgggcaggt gagcaggagc
      181 agacaccgcc ggaaggtttt gaatcctccg gctccgagac ggtgctgggt acggaagtga
      241 aatatgacac gccgatcacc cgcaccatca cgtcggcaaa catcgaccgt ctgcgcttta
      301 ccttcggtgt gcaggcactg gtggaaacca cctcaaaggg ggaccggaat ccgtcggaag
      361 tccgcctgct ggttcagata ca
//
LOCUS       DAZGLJ010000711          381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.724, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000711 DAZGLJ010000000
VERSION     DAZGLJ010000711.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.724"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..77)
                     /gene="hokE"
                     /locus_tag="ACHK49_005566"
     CDS             complement(<1..77)
                     /gene="hokE"
                     /locus_tag="ACHK49_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO9265974.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVG"
BASE COUNT          103 a          105 c           78 g           95 t
ORIGIN      
        1 tctccgacca gaagcgtaaa tcccagtacc gtcaaacaca gcactattac tgccacaagg
       61 gcatatttcg tcagcatgtt tatattgcct ccatgcgaag aagggactac catccgagtt
      121 gttgaggctc ggagtggcag ccccaggatt gatagaaata tctcctgggg cttcatcttt
      181 ccagacctca gagttaacct gaaaccggaa agcttcaggc acccgccgtt atcttaccct
      241 tcctcaacaa aatatctgtt tatttataca gtgaatcctg tttcggactt ctgtcggcaa
      301 aactcatcat tgctcttaaa acgaaatcgc ccgcagccat cactgaccgc gggcgaaagt
      361 gtaaagcagg tgccttacca t
//
LOCUS       DAZGLJ010000712          381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.725, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000712 DAZGLJ010000000
VERSION     DAZGLJ010000712.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.725"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>381
                     /locus_tag="ACHK49_005567"
     CDS             <1..>381
                     /locus_tag="ACHK49_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO9265975.1"
                     /translation="FEHPPEMVESGAHLLMENLAMVNPNLGYSVDEAFLYREYRKARE
                     AGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQQGRRVSLDDILQRADVVTVGIDG
                     GGLDDLLGMYVTGRDRETREWLGWG"
BASE COUNT           72 a           90 c          138 g           81 t
ORIGIN      
        1 tttgagcatc ctcctgaaat ggtggaaagc ggggctcacc tgctgatgga aaacctcgcc
       61 atggttaacc cgaatctcgg ttattcggtg gatgaggcct ttctgtaccg ggagtaccgt
      121 aaagcccggg aggctggtga ggaagcattt cgtggcttca tgtcaaaaca cgccaatgtg
      181 gaaatcggtc ttgccctgcg ttctgaccgc tgggcgggcg cggatttctg ggagcagcag
      241 ggcaggcgcg tcagcctgga cgatatcctg cagcgcgctg atgtggtgac ggtggggatt
      301 gacggcgggg gcctggatga tctgctggga atgtacgtga ctggccgtga cagggaaacc
      361 cgcgaatggc tgggctgggg c
//
LOCUS       DAZGLJ010000713          373 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.726, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000713 DAZGLJ010000000
VERSION     DAZGLJ010000713.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.726"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..198
                     /locus_tag="ACHK49_005568"
     CDS             <1..198
                     /locus_tag="ACHK49_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO9265976.1"
                     /translation="LWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEET
                     TRPTLKELAAKLSGGVNGYSR"
     gene            182..>373
                     /locus_tag="ACHK49_005569"
     CDS             182..>373
                     /locus_tag="ACHK49_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO9265977.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDDLN
                     RAINDAESVLGTTSRRRRPL"
BASE COUNT           89 a           91 c          120 g           73 t
ORIGIN      
        1 ctgtgggata acaaaaagcg gcgtaacgaa gcgctggact gcctggtgta tgcctacgcg
       61 gcattacgtg tgtccgtgca acgctggcag cttgatctgg ctgtactggc aaaatcccgg
      121 gaagaagaga cgacccggcc aaccctgaaa gaactggcag cgaagctgtc cggaggagtg
      181 aatggttaca gtcgctgaac tgcaggcgct gcgtcaggcg cgccttgatt tattaaccgg
      241 taaacgggtg gtgtctgtcc agaaagatgg acgaagaatt gaatatacgg cggcctctct
      301 ggatgatctt aaccgggcga tcaatgatgc ggagtcagtg ctggggacaa caagccgccg
      361 ccgtcgtccg ctg
//
LOCUS       DAZGLJ010000714          368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.727, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000714 DAZGLJ010000000
VERSION     DAZGLJ010000714.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.727"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>368
                     /locus_tag="ACHK49_005570"
     CDS             <1..>368
                     /locus_tag="ACHK49_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265978.1"
                     /translation="MLEMNDDIAKRSLFSLQVQIAEKIRRMTIPVDNIINIYPDLHKK
                     NDNDLSMSIKGFLASNPHTKINILYSNKTEHNIFIKDLFSFAVMENELRDIINNMSKD
                     KTPENWEGRVMLQRYLELKM"
BASE COUNT          147 a           52 c           65 g          104 t
ORIGIN      
        1 atgctagaga tgaatgatga tatagccaaa aggtcattgt tttcattgca agttcaaata
       61 gccgaaaaaa ttcggaggat gaccattcct gtagacaata taattaacat ctatcctgat
      121 ctacataaaa aaaatgacaa tgatctgagt atgtccataa aaggctttct tgcgagtaat
      181 ccacatacaa aaataaatat tctttatagc aataagactg agcataatat ttttataaag
      241 gatttattct ccttcgcagt tatggaaaat gagttaagag acattatcaa taacatgagc
      301 aaggataaga ctcctgaaaa ctgggaaggg agggtaatgt tacaaagata tctcgaatta
      361 aaaatgaa
//
LOCUS       DAZGLJ010000715          364 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.728, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000715 DAZGLJ010000000
VERSION     DAZGLJ010000715.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.728"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            173..>364
                     /locus_tag="ACHK49_005571"
     CDS             173..>364
                     /locus_tag="ACHK49_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9265979.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGT"
BASE COUNT           92 a           89 c           91 g           92 t
ORIGIN      
        1 agagaaaatc ggccagtttt ctctgcctgc agtccgcatg ccgtatcggg ccttgggttc
       61 taacctgttg cgtagattta tgcagcggac tgcctttctc ccaaagtgat aaaccggaca
      121 gtatcatgga ccggttttcc cggtaatccg tatttgcaag gttggtttca ctatggaaca
      181 tgaacttcat tatatcggta tcgacaccgc taaagagaaa ctggatgtcg atgtgttgcg
      241 tcctgatggt cgtcatcgca ccaaaaaatt cgctaacacc actaaagggc acgatgagct
      301 ggtgagctgg ctgaaaggtc acaagattga ccatgcgcat atctgcatcg aagcgaccgg
      361 cacc
//
LOCUS       DAZGLJ010000716          363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.729, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000716 DAZGLJ010000000
VERSION     DAZGLJ010000716.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.729"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..230)
                     /locus_tag="ACHK49_005572"
     CDS             complement(<1..230)
                     /locus_tag="ACHK49_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir chaperone family protein"
                     /protein_id="HFO9265980.1"
                     /translation="MNEQFRTDLAHTFGITLEEQTNALSFHDHDGHEWILECAPQSEV
                     LFFYCYLFNNESSKIDKILEINSNRDLLGMFFL"
BASE COUNT          124 a           54 c           67 g          118 t
ORIGIN      
        1 agaaaaaaca taccgagtag atctcgattg ctatttattt ctagtatttt atcaattttt
       61 gaagattcat tattgaataa ataacaataa aaaaacagga cctcagattg aggtgcacac
      121 tccagtatcc attcatgacc atcgtgatca tggaaagata gggcgttagt ttgttcttca
      181 agagttattc cgaacgtatg tgcaagatct gtgcgaaatt gttcattcat attgtagtcc
      241 tgtcattctt tcaatgaggg tatgcttaaa cgtaattaac tcgtgagctc tggcacaaat
      301 aataagacga aaatataaga tgcaagtaat agtgttgcat atttatgata gcatgtgata
      361 aat
//
LOCUS       DAZGLJ010000717          363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.730, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000717 DAZGLJ010000000
VERSION     DAZGLJ010000717.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.730"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..339)
                     /locus_tag="ACHK49_005573"
     CDS             complement(<1..339)
                     /locus_tag="ACHK49_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO9265981.1"
                     /translation="MIQSTRISMGLFFKYCLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYF"
BASE COUNT          136 a           47 c           68 g          112 t
ORIGIN      
        1 gaaatatttc attaacatga aaagaaaaat gacggcgtta aggaaaaaca aagccacaac
       61 aaccatcagg taattcatct gtgggatgag ttttacattt gtgttttcgt tatataatgt
      121 ggtgatgcaa aataaaatta acgtcagcaa taaactgaaa ataaaaaagc tttgagcttt
      181 taatttttgt gtacccatag aaaccataaa aaggagagca atgtgagtgc tggattttat
      241 tgatggcaga gatatatagt tttgaccagg atcaattttc gttaacgata aacaatattt
      301 aaagaataac cccatggaaa tacgtgttga ctgaatcatt tttccgaatg attgttttct
      361 gga
//
LOCUS       DAZGLJ010000718          358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.731, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000718 DAZGLJ010000000
VERSION     DAZGLJ010000718.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.731"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>358
                     /locus_tag="ACHK49_005574"
     rRNA            <1..>358
                     /locus_tag="ACHK49_005574"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          109 a           81 c          112 g           56 t
ORIGIN      
        1 cataggttaa tgaggcgaac cgggggaact gaaacatcta agtaccccga ggaaaagaaa
       61 tcaaccgaga ttcccccagt agcggcgagc gaacggggag cagcccagag cctgaatcag
      121 tatgtgtgtt agtggaagcg tctggaaagg cgcgcgatac agggtgacag ccccgtacac
      181 aaaaatgcac atgctgtgag ctcgatgagt agggcgggac acgtggtatc ctgtctgaat
      241 atggggggac catcctccaa ggctaaatac tcctgactga ccgatagtga accagtaccg
      301 tgagggaaag gcgaaaagaa ccccggcgag gggagtgaaa aagaacctga aaccgtgt
//
LOCUS       DAZGLJ010000719          356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.732, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000719 DAZGLJ010000000
VERSION     DAZGLJ010000719.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.732"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>356
                     /locus_tag="ACHK49_005575"
     CDS             <1..>356
                     /locus_tag="ACHK49_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO9265982.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEACDENTIGVVP"
BASE COUNT           81 a           94 c          106 g           75 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgcc
//
LOCUS       DAZGLJ010000720          356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.733, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000720 DAZGLJ010000000
VERSION     DAZGLJ010000720.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.733"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            19..95
                     /locus_tag="ACHK49_005576"
     tRNA            19..95
                     /locus_tag="ACHK49_005576"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53..55,aa:Ile,seq:gat)
     gene            138..213
                     /locus_tag="ACHK49_005577"
     tRNA            138..213
                     /locus_tag="ACHK49_005577"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:171..173,aa:Ala,seq:tgc)
BASE COUNT           98 a           77 c           87 g           94 t
ORIGIN      
        1 gagcagtaaa acctctacag gcttgtagct caggtggtta gagcgcaccc ctgataaggg
       61 tgaggtcggt ggttcaagtc cactcaggcc taccaaattt gcacggcaaa tttgaagagg
      121 ttttaactac atgttatggg gctatagctc agctgggaga gcgcctgctt tgcacgcagg
      181 aggtctgcgg ttcgatcccg catagctcca ccatctctgt agtgattaaa taaaaaatac
      241 ttcagagtgt acctgcaaag gttcactgcg aagttttgct ctttaaaaat ctggatcaag
      301 ctgaaaattg aaacactgaa caacgaaagt tgttcgtgag tctctcaaat tttcgc
//
LOCUS       DAZGLJ010000721          356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.734, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000721 DAZGLJ010000000
VERSION     DAZGLJ010000721.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.734"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>356
                     /locus_tag="ACHK49_005578"
     CDS             <1..>356
                     /locus_tag="ACHK49_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO9265983.1"
                     /translation="FEHPPEMVESGANLLMENLAMVNPNLGYSVDEAFLYREYRKARE
                     AGEETFRGFMSKHANVEIGLALRSDRWAGADFWEEQGRCISLDDILRRADVVTVGIDG
                     GGLDDLLGMYVTGRDR"
BASE COUNT           72 a           79 c          125 g           80 t
ORIGIN      
        1 tttgaacacc ctcctgaaat ggtggaaagc ggggctaacc tgctgatgga aaacctcgcc
       61 atggtcaatc cgaatctcgg ctattcagtg gatgaggcct ttctgtaccg ggagtaccgt
      121 aaagcccggg aagccggtga agagacattc cgggggttca tgtcaaaaca cgccaatgtg
      181 gaaattggtc ttgccctgcg ctctgaccgc tgggcggggg ctgatttctg ggaagagcag
      241 ggccgttgta tcagcctgga cgatatcctg cgtcgtgctg atgtggtgac ggtggggatt
      301 gacggcggag ggctggatga tctgctgggg atgtatgtga ctgggcgtga ccggga
//
LOCUS       DAZGLJ010000722          353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.735, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000722 DAZGLJ010000000
VERSION     DAZGLJ010000722.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.735"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>353
                     /locus_tag="ACHK49_005579"
     CDS             <1..>353
                     /locus_tag="ACHK49_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein O"
                     /protein_id="HFO9265984.1"
                     /translation="SPKTRDKTSLKLGDCYPSKQGDTKDTITKEKRKDYSSENSGESS
                     DQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIR
                     LMRERDGRNHRDMCVL"
BASE COUNT          114 a           82 c           89 g           68 t
ORIGIN      
        1 tcccctaaaa cgagggataa aacatccctc aaattagggg attgctatcc ctcaaaacag
       61 ggggacacaa aagacactat tacaaaagaa aaaagaaaag attattcgtc cgagaattct
      121 ggcgaatcct ctgaccagcc agaaaacgat ctttctgtgg ttaaaccgga tgctgcaatt
      181 cagagcggca gcaagtgggg aacagcagaa gacctgaccg ccgcagagtg gatgtttgac
      241 atggtgaaga ccatcgcacc atcagccaga aaaccgaatt ttgcagggtg ggctaacgat
      301 atccgcctga tgcgtgaacg tgacggacgt aaccaccgcg atatgtgtgt gct
//
LOCUS       DAZGLJ010000723          350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.736, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000723 DAZGLJ010000000
VERSION     DAZGLJ010000723.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.736"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            55..264
                     /gene="fumD"
                     /locus_tag="ACHK49_005580"
     CDS             55..264
                     /gene="fumD"
                     /locus_tag="ACHK49_005580"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO9265985.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          113 a           58 c           91 g           88 t
ORIGIN      
        1 taattggtta gattgatata tgctgatagt tggcatgata aataggtata aagaatggga
       61 aatcggacaa aagaggatga actgtaccga gaaatgtgca gagttgtcgg taaagttgtg
      121 ctggaaatgc gcgatctggg gcaggaacca aagcatattg ttatcgcagg cgtattacgt
      181 acagcattag ccaataaacg cattcagcgc agtgagctgg aaaagcaggc gatggaaacc
      241 gtcattaacg cactggtaaa atgaccattc aggatgaggc ggtaagcttc atcctgatat
      301 tcttgcttca gttgcggtcc agttaatcat tctaaataac aactgctgaa
//
LOCUS       DAZGLJ010000724          345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.737, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000724 DAZGLJ010000000
VERSION     DAZGLJ010000724.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.737"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..111)
                     /locus_tag="ACHK49_005581"
     CDS             complement(<1..111)
                     /locus_tag="ACHK49_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO9265986.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNT"
     gene            complement(263..>345)
                     /locus_tag="ACHK49_005582"
     CDS             complement(263..>345)
                     /locus_tag="ACHK49_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO9265987.1"
                     /translation="IDLAYDGSGSGDWRSDAFIVGIGYRF"
BASE COUNT           94 a           93 c           71 g           87 t
ORIGIN      
        1 cgtgttcacc accaccgtgc tgctggttcg tctggccttc agcacaatgg tgcagttctg
       61 tactggtttt cctgtgccat ctttaagcac gccagaaatt tttactgtca tacttttcca
      121 ccaataaaaa aagcccgcag cagtgacgcc acgggcttca ggacagtgta actttacgtt
      181 tcctcaaacg cagttcaccc cataaggtgg atgaacctgc gtatcataac aatatttaca
      241 gaagataaat cggcgtctgt tgtcagaaac ggtatccgat accaacaata aatgcatccg
      301 atcgccagtc gccactaccg gaaccgtcat aagcaaggtc aatgg
//
LOCUS       DAZGLJ010000725          345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.738, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000725 DAZGLJ010000000
VERSION     DAZGLJ010000725.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.738"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..342)
                     /locus_tag="ACHK49_005583"
     CDS             complement(<1..342)
                     /locus_tag="ACHK49_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265988.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDIPESPTGNNLEALAPGIEKPKQ
                     TSIEMVTLLNTLQPDGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLK"
BASE COUNT          102 a           54 c           67 g          122 t
ORIGIN      
        1 ctttaatggt tcattaatgg taagttcgca ggtattgtcc gatcgttgaa taatttgggc
       61 gttataacct aaaaaatcca gacgaagaga cgagtcatta taaagaatta cattttgtaa
      121 gtacgctaat tctttttgaa aatcaccagt gataatgcat tttccatcag gttgtaacgt
      181 gttaagtaaa gtgaccattt caatagaggt ctgttttggt ttttctatac ctggtgctag
      241 tgcttcgagg ttatttcctg ttgggctttc aggaatgtca ttcatttttc gacggactaa
      301 atcactacta atatcattac caattgatga aacagggaac attat
//
LOCUS       DAZGLJ010000726          338 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.739, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000726 DAZGLJ010000000
VERSION     DAZGLJ010000726.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.739"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>338
                     /locus_tag="ACHK49_005584"
     CDS             <1..>338
                     /locus_tag="ACHK49_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO9265989.1"
                     /translation="IPPAVQHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGS
                     GEDSRLVTTAITADTEHRSSGLPLGEYTLTVRAINSYGQQGEPATTTFRINAPAKPAT
                     IELTPGYFQIT"
BASE COUNT           79 a          101 c          105 g           53 t
ORIGIN      
        1 attccaccgg cagtgcagca cctcacggtg gaggtgagcg cggctgacgg ccagtatctg
       61 gcacaggcga aatgggacac gccgcgggtg gtgaaggggg tgcgcttcag tctgcgactg
      121 accagcggaa gcggagaaga cagccgtctg gtgaccaccg ccatcactgc ggatacagag
      181 catcgttcca gtggtctgcc gctcggggaa tacaccctga cagtcagggc aattaacagt
      241 tatggccagc agggcgaacc ggccaccacc accttccgga ttaacgcgcc agcaaaaccc
      301 gccaccattg aactgacgcc ggggtatttt cagataac
//
LOCUS       DAZGLJ010000727          336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.740, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000727 DAZGLJ010000000
VERSION     DAZGLJ010000727.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.740"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            1..336
                     /locus_tag="ACHK49_005585"
                     /pseudo
     CDS             1..336
                     /locus_tag="ACHK49_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT          112 a           59 c           56 g          109 t
ORIGIN      
        1 cattaaggtg gtatatttta taaataatgt cgccgtccat aataatacta tcgaaattcc
       61 acagacagta aatggtggtt acgatttttc acaccttagc ctgaaaggta tcgtgattaa
      121 agatgaagat ttatccaatt cgaattttgc aggttgctga ctacaaaacg ctattttcca
      181 ggactgtaat atgtataaaa cgaattttta ttacgccata atggaaaaaa tactttttga
      241 taattgtatt ctcgatgact caaatttcgc tcagataaaa atggccgacg gaactctaaa
      301 tgcatgctcc gctatgcatg ttcaattcta caatgc
//
LOCUS       DAZGLJ010000728          326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.741, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000728 DAZGLJ010000000
VERSION     DAZGLJ010000728.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.741"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..249
                     /locus_tag="ACHK49_005586"
     CDS             <1..249
                     /locus_tag="ACHK49_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO9265990.1"
                     /translation="AGDPDTLLGLPVEYLEFMPDNVIALGDFKRGYYIVDHETGVRTR
                     PDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPQDDD"
BASE COUNT           89 a           69 c           86 g           82 t
ORIGIN      
        1 gcgggtgatc ctgatacgtt gctgggcctt ccggtcgaat atctggagtt tatgcctgat
       61 aacgttattg ccctgggtga cttcaaacgc ggttactaca ttgttgatca cgaaacaggt
      121 gttcgcacca gaccggacaa cctcacagag ccaggcttca tcaaaatttt cacgcagaaa
      181 tatttaggcg gtggcgtggt ggattcgaac gcgatcaaga ttctggaact gccacaggac
      241 gacgattaac agcatacaga aggggcttaa aagccccttt agtgttttat gggtgaaaaa
      301 attatgaaga gtatggaaat ccggtc
//
LOCUS       DAZGLJ010000729          326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.742, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000729 DAZGLJ010000000
VERSION     DAZGLJ010000729.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.742"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>326)
                     /locus_tag="ACHK49_005587"
     CDS             complement(<1..>326)
                     /locus_tag="ACHK49_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO9265991.1"
                     /translation="DSEPLKALPQSNIWPVSIYYRLLSFDYFSARLDSLLYLDADIVC
                     KGSLNELIALEFKDEYGAVVIDVDAMQSKSAERLCNEDFNGSYFNSGVMYINLREWLK
                     QRLTEK"
BASE COUNT          116 a           72 c           41 g           97 t
ORIGIN      
        1 tttttccgtt agtctttgtt ttaaccattc ccgtaaatta atatacatta caccagagtt
       61 aaaatagcta ccgttaaaat cctcattaca caaacgctca gcgcttttac tttgcatagc
      121 atctacatca attaccactg ccccatattc atctttaaac tctaatgcta ttaactcgtt
      181 caatgaaccc ttacagacga tatcagcatc aagatataat aatgaatcca atcgcgcaga
      241 aaaataatca aatgaaagca aacgataata aatagatact ggccagatat ttgattgagg
      301 tagcgccttc aatggctcag aatcaa
//
LOCUS       DAZGLJ010000730          321 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.743, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000730 DAZGLJ010000000
VERSION     DAZGLJ010000730.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.743"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            4..>321
                     /locus_tag="ACHK49_005588"
     CDS             4..>321
                     /locus_tag="ACHK49_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265992.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIK"
BASE COUNT          112 a           60 c           74 g           75 t
ORIGIN      
        1 attatggtta tcagtacagt gggttatgaa aaacggactg gatctgccag cagtgcttct
       61 attattattc aaaaagaact tcagccggac aatgctataa tccagggaaa ggctataagc
      121 tctgttggac ggaatgtgga aatcagcact ggtaaaaggg gaactgacat taacacaaga
      181 aggattcctg caagcaactg cttatctgcc cggaacgcag aattaaagca taaccacaca
      241 ggagccagaa tactgtggtc gtacgcattc agtattgtga caactaatag aaataaaaat
      301 ctggccggtg cgaatattaa a
//
LOCUS       DAZGLJ010000731          311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.744, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000731 DAZGLJ010000000
VERSION     DAZGLJ010000731.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.744"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            18..65
                     /locus_tag="ACHK49_005589"
     CDS             18..65
                     /locus_tag="ACHK49_005589"
                     /inference="COORDINATES: protein motif:HMM:NF017611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO9265993.1"
                     /translation="MSDPNSSMYEEEYDG"
     gene            58..>311
                     /locus_tag="ACHK49_005590"
     CDS             58..>311
                     /locus_tag="ACHK49_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO9265994.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCCPEHGTQLALERRSKERE
                     KAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQA"
BASE COUNT          121 a           66 c           78 g           46 t
ORIGIN      
        1 actgctgtgc agaactgatg agcgatccga atagctcaat gtacgaggaa gaatacgatg
       61 gctaaaccag cgagaaggaa atgcaaaata tgcaaggaat ggtttcaccc ggcattctca
      121 aatcagtggt ggtgctgccc ggaacacgga actcaattag cactcgaacg acgaagtaaa
      181 gaacgcgaaa aagcggaaaa agcagcagag aagaaacgac gacgagagga gcagaaacag
      241 aaagataaac ttaagattcg aaaactcgcc ttaaagcccc gcagttactg gattaaacaa
      301 gcccaacaag c
//
LOCUS       DAZGLJ010000732          309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.745, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000732 DAZGLJ010000000
VERSION     DAZGLJ010000732.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.745"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>309
                     /locus_tag="ACHK49_005591"
     CDS             <1..>309
                     /locus_tag="ACHK49_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO9265995.1"
                     /translation="RVKKLILDLQKQFGENRISIKDVNSLTSLSKSENNHNYQTEMLL
                     RWNYPAASDLLRMYILKEHGGIYTDTDMMPAYSKQVIFKIMMQTNGDNRFLEDLKLRR
                     A"
BASE COUNT          111 a           55 c           50 g           93 t
ORIGIN      
        1 tagagtaaaa aaactaattc ttgatttaca gaaacaattt ggtgaaaacc gtatttcaat
       61 taaagatgtt aactctttaa cctctctttc aaaatcagaa aataatcaca attatcaaac
      121 tgaaatgttg cttcgatgga actatcctgc cgcctcagac ctgctcagga tgtatatcct
      181 taaagagcat ggtggtattt atacagacac agacatgatg cctgcatact ctaaacaagt
      241 aatttttaaa attatgatgc agacaaacgg agataatcgt ttcttggaag atttgaaact
      301 acgtcgtgc
//
LOCUS       DAZGLJ010000733          309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.746, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000733 DAZGLJ010000000
VERSION     DAZGLJ010000733.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.746"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>309
                     /locus_tag="ACHK49_005592"
     CDS             <1..>309
                     /locus_tag="ACHK49_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9265996.1"
                     /translation="LAARKARAAPLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWD
                     ALNVYCSNGWVEIDNNIAENALRGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNN
                     V"
BASE COUNT           83 a           74 c           94 g           58 t
ORIGIN      
        1 tctggcggca agaaaagcca gagccgcgcc actgatgcag tcactgtatg actggataca
       61 gcaacagatg aaaacactgt cgcgtcactc agatacggca aaagcgttcg catacctgct
      121 gaaacagtgg gatgcactga acgtgtactg cagtaatggc tgggtggaaa tcgacaacaa
      181 catcgcagag aacgccttac ggggagtggc cgtaggccgg aaaaactgga tgttcgcggg
      241 ttccgacagc ggtggtgaac atgcggcggt gttgtactcg ctgatcggca catgccgtct
      301 gaacaatgt
//
LOCUS       DAZGLJ010000734          308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.747, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000734 DAZGLJ010000000
VERSION     DAZGLJ010000734.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.747"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>308)
                     /gene="escQ"
                     /locus_tag="ACHK49_005593"
     CDS             complement(<1..>308)
                     /gene="escQ"
                     /locus_tag="ACHK49_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HFO9265997.1"
                     /translation="NDFYLPLLSVIGTGRLYITPEGHACHAYFREVSGNGIRFTLTCS
                     GYEGRFWISEEQFIQWCQELFPYSESRLIPEDMIKLMILWVMQTALPEGDVSVDDVQF
                     "
BASE COUNT           95 a           77 c           58 g           78 t
ORIGIN      
        1 taaattgaac atcatcaaca gacacatcac cttctggtaa agcagtctgc attacccata
       61 agatcatgag ttttatcata tcttccggta tcagccggct ttcgctatac gggaataact
      121 cctggcacca ctgaatgaac tgttcttctg atatccaaaa acgcccctca tagcctgagc
      181 atgttaacgt gaagcggata ccattaccgc tgacttcgcg aaaatatgca tggcaagcat
      241 gcccttcagg cgtaatatag aggcgaccgg ttcctatgac tgacaacaat ggcaagtaaa
      301 aatcatta
//
LOCUS       DAZGLJ010000735          307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.748, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000735 DAZGLJ010000000
VERSION     DAZGLJ010000735.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.748"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(8..286)
                     /locus_tag="ACHK49_005594"
     CDS             complement(8..286)
                     /locus_tag="ACHK49_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFO9265998.1"
                     /translation="MPFSIKSIFSGHTWHQPEISRPIADKSSTKNCILDSTTCNVDGF
                     TVFNRRSCSFDMRPPGSADRTPQLRLSISEVAWMSKIIETETNNTNKS"
BASE COUNT           80 a           52 c           55 g          120 t
ORIGIN      
        1 ctaatagcta tgatttgttt gtattatttg tctctgtctc tattattttt gacatccatg
       61 cgacttctga tattgataat ctcagttgtg gagttctgtc tgcgcttccg ggaggacgca
      121 tatcaaaact acaggatctt ctgttaaaca ctgtaaagcc atctacattg catgttgttg
      181 agtctaagat acaattttta gtagatgatt tatctgctat aggcctagat atttcaggtt
      241 gatgccaagt gtgtcctgaa aaaatacttt ttattgaaaa tggcattttt atcctcacat
      301 tatgtct
//
LOCUS       DAZGLJ010000736          306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.749, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000736 DAZGLJ010000000
VERSION     DAZGLJ010000736.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.749"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>306)
                     /locus_tag="ACHK49_005595"
     CDS             complement(<1..>306)
                     /locus_tag="ACHK49_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9265999.1"
                     /translation="VRFLQGVKQYFCTKALDVILLIYLFLGFILLPYKYLWKDIILSL
                     CFVGILLYALVEENRITEYMGYFVKFSNKNRLNSCAAWCALIACFIFLFLVLSINIF"
BASE COUNT          137 a           57 c           35 g           77 t
ORIGIN      
        1 aaaaaatatt aattgacaac actaaaaaca aaaatataaa acacgcaata agtgcacacc
       61 aggcagcaca actattcaac ctgtttttat tactgaactt aacaaaatac cccatatatt
      121 ctgtaatgcg attttcttcc actaatgcat aaagaagaat accaacaaag cacagagata
      181 aaataatatc tttccacaga tatttataag ggagaagtat aaaaccaaga aatagataaa
      241 ttagcagaat gacatctaat gccttagtac aaaaatattg tttcactcct tgcaagaacc
      301 gaacat
//
LOCUS       DAZGLJ010000737          305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.750, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000737 DAZGLJ010000000
VERSION     DAZGLJ010000737.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.750"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            136..>305
                     /locus_tag="ACHK49_005596"
     CDS             136..>305
                     /locus_tag="ACHK49_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2517 family protein"
                     /protein_id="HFO9266000.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVW"
BASE COUNT           89 a           66 c           70 g           80 t
ORIGIN      
        1 taaaccatca ataagcaaaa ataagtggtc ggatgagtag taaaattaca caaaaggcgg
       61 tactattttc atcagataaa caaattcatt tttccggtac cattcaccgg gtagatacaa
      121 aagggggaga aaagtatgga actctacaga gaatatcctg catggcttat ctttttacgc
      181 cgtacttatg cggttgcagc gggcgttctg gcgctgcctt tcatgctttt ctggaaagac
      241 cgcgcccgct tttacagcta cctgcatcgc gtctggtcga aaaccagcga taaaccggtg
      301 tggat
//
LOCUS       DAZGLJ010000738          303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.751, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000738 DAZGLJ010000000
VERSION     DAZGLJ010000738.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.751"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>303)
                     /locus_tag="ACHK49_005597"
     CDS             complement(<1..>303)
                     /locus_tag="ACHK49_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO9266001.1"
                     /translation="PWWLVCRGEIHKFRCVPHLTGRRFEHGVTDCYTLFRDAYHLAEI
                     EMPDFVREDDWWRNGQNLYLDNLEATGLYQVPLSAAQPGDVLLCCFGSSVPNHAAIY"
BASE COUNT           81 a          103 c           71 g           48 t
ORIGIN      
        1 gtaaatagcg gcgtgattcg gcaccgatga accaaaacag cacagcagca catcgcccgg
       61 ctgcgccgct gacaacggca cctgatacag ccctgtggcc tccagattat ccagatagag
      121 attctgaccg ttacgccacc agtcatcctc acgcacaaaa tccggcatct caatctccgc
      181 cagatgataa gcatcccgga acagtgtgta acagtccgtc acaccgtgct caaagcgccg
      241 cccggtgaga tgcggcacac agcggaactt atgaatctca ccccggcaga ccagccacca
      301 cgg
//
LOCUS       DAZGLJ010000739          298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.752, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000739 DAZGLJ010000000
VERSION     DAZGLJ010000739.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.752"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..>298)
                     /locus_tag="ACHK49_005598"
     CDS             complement(<1..>298)
                     /locus_tag="ACHK49_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9266002.1"
                     /translation="SFFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPK
                     ARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEG"
BASE COUNT           66 a           94 c           79 g           59 t
ORIGIN      
        1 ccccttcgtc tgccgtgctg atctcctgag aaaccacgcg agaccccacg cgcatttccc
       61 cgtacagaac aggcagaaca ttgccctggg caaccatgtt atccagtgag gagaaatagg
      121 tgttctgctt accgttatcc gttgtctgtg tacggggagt tctggctttc ggtgccagca
      181 tctgcgccac accaccgagc accatactgg caccgagaga aaacaggatg ccggtcatac
      241 caccggcccc aatggctgcc ccccatgctg caagggtggc tccggcggta aagaatga
//
LOCUS       DAZGLJ010000740          292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.753, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000740 DAZGLJ010000000
VERSION     DAZGLJ010000740.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.753"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>292
                     /locus_tag="ACHK49_005599"
     CDS             <1..>292
                     /locus_tag="ACHK49_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFO9266003.1"
                     /translation="HPLVIYDAGYKNYHESLNAAIAINSRPLQEIHYGDVLYHYHKND
                     ISLGVDTLYGRESFDIVLDAMNVYRKSKKMRVISNNEMKKSIKISELVIHNII"
BASE COUNT          117 a           35 c           48 g           92 t
ORIGIN      
        1 cacccattag taatatatga tgcgggttac aaaaattatc atgagtcatt aaatgctgct
       61 attgcaataa actctagacc attacaagaa atacattatg gtgatgtttt atatcattat
      121 cataaaaatg acatctcttt gggagtagat actctttacg ggagggaaag ttttgatatt
      181 gtactggatg caatgaacgt atatagaaaa agcaaaaaaa tgagagttat ttccaataat
      241 gagatgaaaa aaagcattaa aatatctgaa ttagttatcc ataatattat aa
//
LOCUS       DAZGLJ010000741          280 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.754, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000741 DAZGLJ010000000
VERSION     DAZGLJ010000741.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.754"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
BASE COUNT           91 a           44 c           65 g           80 t
ORIGIN      
        1 acaaatatgg agagcttgat gagtcagagc taaaacagca attgattaat aaacatgttg
       61 atttattcaa gaaaatggat gtattttaaa tgttgattct taatataacc taatcaatag
      121 attatcaagt gagagctaca cattattgga ggggcagggg aaaacaccat tttgggaaca
      181 atgcatcagg tactcttacc actgaagcaa gatcatccgg caccaggtgg tggtgcagtg
      241 ctttggaaat ggtcccctcg ggggagttgt cattattttc
//
LOCUS       DAZGLJ010000742          276 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.denovo.755, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000742 DAZGLJ010000000
VERSION     DAZGLJ010000742.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.denovo.755"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..>276
                     /locus_tag="ACHK49_005600"
     CDS             <1..>276
                     /locus_tag="ACHK49_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9266004.1"
                     /translation="NKLINFGFTRTDAQVYISLLKNGRSSGYKIAKEISLSRSSVYSS
                     IDNLYKNGYIFLVDGETKEYEAKSPELILSQIEKSTIDNIKILKKELS"
BASE COUNT          103 a           46 c           41 g           86 t
ORIGIN      
        1 aataaattga taaattttgg tttcactcgc accgatgcac aggtttacat cagtctgttg
       61 aaaaacggtc gtagcagtgg ttacaagata gcaaaagaaa tttcactttc tcgttcgtct
      121 gtatactcat ctattgataa tctttacaag aacggttata tctttcttgt cgatggtgaa
      181 actaaagaat atgaagcaaa atctcctgaa ctcatacttt cacaaattga aaaaagcact
      241 attgacaata taaaaattct aaaaaaagaa ttatca
//
LOCUS       DAZGLJ010000743         4406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.guided.299, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000743 DAZGLJ010000000
VERSION     DAZGLJ010000743.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.guided.299"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            5..844
                     /gene="sul1"
                     /locus_tag="ACHK49_005601"
     CDS             5..844
                     /gene="sul1"
                     /locus_tag="ACHK49_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFO9266005.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            972..1472
                     /locus_tag="ACHK49_005602"
     CDS             972..1472
                     /locus_tag="ACHK49_005602"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9266006.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKGEAAYLHKLAVRRTHA
                     GRGVSSALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNPGWDPTFIAE
                     RLELEI"
     gene            complement(1648..2433)
                     /gene="istB"
                     /locus_tag="ACHK49_005603"
     CDS             complement(1648..2433)
                     /gene="istB"
                     /locus_tag="ACHK49_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS1326 family helper ATPase
                     IstB"
                     /protein_id="HFO9266007.1"
                     /translation="MMQHEGHVRILKSLKLFGMAHAIEELGNQNSPAFNQALPMLDSL
                     IKAEVAEREVRSVNYQLRVAKFPVYRDLVGFDFSQSLVNEATVKQLHRCDFMEQAQNV
                     VLIGGPGTGKTHLATAIGTQAVMHLNRRVRFFSTVDLVNALEQEKSSGRQGQIANRLL
                     YADLVILDELGYLPFSQTGGALLFHLLSKLYEKTSVILTTNLSFSEWSRVFGDEKMTT
                     ALLDRLTHHCHILETGNESYRFKHSSTQNKQEEKQTRKLKIET"
     gene            complement(2420..3943)
                     /gene="istA"
                     /locus_tag="ACHK49_005604"
     CDS             complement(2420..3943)
                     /gene="istA"
                     /locus_tag="ACHK49_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS1326 family transposase"
                     /protein_id="HFO9266008.1"
                     /translation="MINVAILSAIRRWHFRDGASIREIARRSGLSRNTVRKYLQSKVV
                     EPQYPARDSVGKLSPFEPKLRQWLSTEHKKTKKLRRNLRSMYRDLVALGFTGSYDRVC
                     AFARQWKDSEQFKAQTSGKGCFIPLRFACGEAFQFDWSEDFARIAGKQVKLQIAQFKL
                     AHSRAFVLRAYYQQKHEMLFDAHWHAFQIFGGIPKRGIYDNMKTAVDSVGRGKERRVN
                     QRFTAMVSHYLFDAQFCNPASGWEKGQIEKNVQDSRQRLWQGAPDFQSLADLNVWLEH
                     RCKALWSELRHPELDQTVQEAFADEQGELMALPNAFDAFVEQTKRVTSTCLVHHEGNR
                     YSVPASYANRAISLRIYADKLVMAAEGQHIAEHPRLFGSGHARRGHTQYDWHHYLSVL
                     QKKPGALRNGAPFAELPPAFKKLQSILLQRPGGDRDMVEILALVLHHDEGAVLSAVEL
                     ALECGKPSKEHVLNLLGRLTEEPPPKPIPIPKGLRLTLEPQANVNRYDSLRRAHDAA"
     gene            4066..>4406
                     /locus_tag="ACHK49_005605"
     CDS             4066..>4406
                     /locus_tag="ACHK49_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9266009.1"
                     /translation="MYSYEDRLRAVRLYLKLGRRMSATLRQLGYPTKNSLKAWLAEFE
                     RNQDLRRGYQRIKRQYTDEQKQRAVDHYIEQGYCLSHTIRSLGYPSREALRAWIRDLR
                     PEFARTVVGSSA"
BASE COUNT          920 a         1263 c         1223 g         1000 t
ORIGIN      
        1 cgccatggtg acggtgttcg gcattctgaa tctcaccgag gactccttct tcgatgagag
       61 ccggcggcta gaccccgccg gcgctgtcac cgcggcgatc gaaatgctgc gagtcggatc
      121 agacgtcgtg gatgtcggac cggccgccag ccatccggac gcgaggcctg tatcgccggc
      181 cgatgagatc agacgtattg cgccgctctt agacgccctg tccgatcaga tgcaccgtgt
      241 ttcaatcgac agcttccaac cggaaaccca gcgctatgcg ctcaagcgcg gcgtgggcta
      301 cctgaacgat atccaaggat ttcctgaccc tgcgctctat cccgatattg ctgaggcgga
      361 ctgcaggctg gtggttatgc actcagcgca gcgggatggc atcgccaccc gcaccggtca
      421 ccttcgaccc gaagacgcgc tcgacgagat tgtgcggttc ttcgaggcgc gggtttccgc
      481 cttgcgacgg agcggggtcg ctgccgaccg gctcatcctc gatccgggga tgggattttt
      541 cttgagcccc gcaccggaaa catcgctgca cgtgctgtcg aaccttcaaa agctgaagtc
      601 ggcgttgggg cttccgctat tggtctcggt gtcgcggaaa tccttcttgg gcgccaccgt
      661 tggccttcct gtaaaggatc tgggtccagc gagccttgcg gcggaacttc acgcgatcgg
      721 caatggcgct gactacgtcc gcacccacgc gcctggagat ctgcgaagcg caatcacctt
      781 ctcggaaacc ctcgcgaaat ttcgcagtcg cgacgccaga gaccgagggt tagatcatgc
      841 ctagcattca ccttccggcc gcccgctagc ggaccctggt caggttccgc gaaggtgggc
      901 gcagacatgc tgggctcgtc aggatcaaac tgcactatga ggcggcggtt cataccgcgc
      961 caggggagcg aatggacagc gaggagcctc cgaacgttcg ggtcgcctgc tcgggtgata
     1021 tcgacgaggt tgtgcggctg atgcacgacg ctgcggcgtg gatgtccgcc aagggaacgc
     1081 ccgcctggga cgtcgcgcgg atcgaccgga cattcgcgga gaccttcgtc ctgagatccg
     1141 agctcctagt cgcgagttgc agcgacggca tcgtcggctg ttgcaccttg tcggccgagg
     1201 atcccgagtt ctggcccgac gccctcaagg gggaggccgc atatctgcac aagctcgcgg
     1261 tgcgacggac acatgcgggc cggggtgtca gctccgcgct gatcgaggct tgccgccatg
     1321 ccgcgcgaac gcaggggtgc gccaagctgc ggctcgactg ccacccgaac ctgcgtggcc
     1381 tatacgagcg gctcggattc acccacgtcg acactttcaa tcccggctgg gatccaacct
     1441 tcatcgcaga acgcctagaa ctcgaaatct aacgtccgtt cgggcatcga ggtccatgtc
     1501 ggggtgggac gggcccgtgg cttcaagatc acttgcagtc cgaccgcgat gtcttggttg
     1561 cgcgagaggt tgtcgatatc tgttgatttg cacccaaatt tgacccggga tttgcattga
     1621 attttgaccc accccttgtt gtcagaatta tgtctcgatt ttcagtttgc gggtctgttt
     1681 ttcctcctgc ttattctgag ttgaactgtg tttgaagcgg taactttcat tgccggtttc
     1741 caggatgtgg cagtggtggg ttagtcggtc caacaacgct gttgtcatct tttcatcgcc
     1801 aaacactcgg ctccattccg agaagctcaa gttggtggtc agtatcacgc tggttttttc
     1861 gtacagcttt gagagcaggt gaaacagcag tgccccaccg gtttggctaa aaggcaaata
     1921 tcccagctca tccagaatca ccaaatcggc atacaacaga cggtttgcga tttgtccctg
     1981 acgcccagat gatttctctt gctccagtgc attgaccaaa tccacggtgg agaagaaacg
     2041 cacccgtcgg ttcaagtgca tcactgcttg tgtaccaatg gctgtggcca ggtgagtctt
     2101 gcctgtgcct ggcccaccaa tcagcaccac gttctgggct tgttccatga agtcgcaccg
     2161 gtgcaattgt ttgaccgtgg cctcattaac caggctttga ctgaagtcaa agcccaccaa
     2221 gtcccgatac acggggaact tggccacccg caattgatag ttcaccgaac gtacttcacg
     2281 ctctgccact tcagctttaa tcaagctgtc cagcatgggc aaggcttgat taaatgctgg
     2341 tgaattctga ttgcccaact cctcaatggc gtgtgccatg ccaaagagtt tcaaggattt
     2401 gaggattctc acatggcctt catgctgcat catgggctct ccttaaactg tcatagcggt
     2461 tcacgttggc ctgtggttcc aatgtcagcc ttaacccctt gggaattgga atcggtttgg
     2521 gtggaggttc ttcggtcaaa cgtcccaaca gattaagcac atgctccttc gatggcttgc
     2581 cacactccaa tgccaattcc acagcactga gtaccgcacc ttcatcgtgg tgcaatacaa
     2641 gggccagaat ttccaccatg tcacggtcac cgccggggcg ttgcagcaag atggattgaa
     2701 gcttcttgaa cgcgggtggc aattcagcaa atggcgcacc attgcgcaac gccccaggtt
     2761 tcttctgaag cacagacaag taatggtgcc agtcgtattg tgtgtggcca cgccgagcgt
     2821 ggccactgcc aaacaatctt ggatgctcgg caatgtgttg gccttcggca gccatcacca
     2881 gcttgtctgc ataaatccga aggctgatgg ccctgttggc gtaactggca ggaacgctgt
     2941 agcgattgcc ctcgtggtga acaaggcagg ttgaagtgac tcgcttggtt tgctccacga
     3001 atgcatcaaa ggcattgggt agcgccatca actcgccttg ttcatcggca aaggcctctt
     3061 gcacggtttg gtccaattcg gggtggcgca gctcagacca cagcgctttg cagcgatgct
     3121 caagccacac attcaaatca gcaaggcttt gaaagtctgg tgccccttgc cacaggcgtt
     3181 ggcgggaatc ctgcacgttc ttctcaatct ggcctttctc ccaacccgat gctggattac
     3241 agaactgcgc atcaaacagg tagtggctga ccatggcagt gaaccgctga ttgaccctgc
     3301 gctctttgcc acgccccacc gaatccacag cggtcttcat gttgtcgtag atgccgcgct
     3361 tgggaatgcc accgaagatt tgaaaggcat gccagtgggc atcaaacagc atttcatgtt
     3421 tttgctggta gtaagcccga agcacaaagg cccggctgtg ggccaactta aactgggcaa
     3481 tctgaagttt gacctgtttg cccgctatgc gggcaaagtc ctcactccaa tcgaattgga
     3541 aggcttcgcc acaagcaaag cgcaagggga tgaaacaacc cttgcccgag gtttgcgcct
     3601 tgaactgttc ggaatctttc cactgtcggg caaaggcaca cactcggtca taagacccgg
     3661 taaagcccaa agcgaccaaa tcccggtaca tgctgcgcag gtttctgcgc agcttctttg
     3721 tctttttgtg ctcggtggag agccactgcc ttaacttggg ctcaaaagga cttaatttgc
     3781 caacgctgtc tcgcgctggg tactgcggtt caaccacctt gctttgcaaa tacttgcgaa
     3841 cggtgttcct ggacaggccg cttcgtcggg ctatttcccg aatcgacgca ccatcgcgaa
     3901 aatgccagcg tcgaattgcg ctcaatatcg ccacgtttat cactccttga tttctcccgc
     3961 catatccaga cgggaaacag tgtcatacgt gggtcaaatt tcgacgcaaa tctttaccct
     4021 aagttggggt gcggacaaaa tcttggacta ctttaggagt agttcatgta ttcgtatgaa
     4081 gatcgccttc gagccgtgag gttgtacctg aagcttgggc gccggatgag cgccacacta
     4141 cggcagctgg gataccccac caagaactcg ctgaaggcct ggttggcaga attcgaacgg
     4201 aatcaggatc ttcgccgagg ctatcaacgg ataaaacggc agtacaccga tgagcaaaag
     4261 caacgggcag tagatcacta tatcgaacaa ggctactgcc tgagtcacac aatccgaagc
     4321 ctgggctacc caagccgcga ggccttgcgt gcctggatcc gtgatttacg ccctgaattc
     4381 gctaggacgg tcgtcggcag cagcgc
//
LOCUS       DAZGLJ010000744         3755 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.guided.316, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000744 DAZGLJ010000000
VERSION     DAZGLJ010000744.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.guided.316"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..894
                     /locus_tag="ACHK49_005606"
     CDS             <1..894
                     /locus_tag="ACHK49_005606"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFO9266010.1"
                     /translation="LPLLDIASQCPSLLASYALGTTAANARALDSTEERIQDYRREQR
                     ILSDNSISGQSVQRGTLPPDTRPYLAPDWPTLAENTMPSVIALSAIEQPYGFAENSPA
                     WLIPGVQQNAATAALTHYRAWQKGWAKQDAKRQHQTICGGFVIEGNRVLTAAHCVDRQ
                     EALRIRTAGGEWRAAHVVGADVTQDVALLEFEGDPLPPIKVANTLPRPGQDVMAIGSP
                     SGYGFAVGVGIVAWHGLDAKMLSPNDFMLVTASVVGGNSGGVVVNTHGEAVSLVSYGS
                     GSYTQTVPIDRALTVAGQLAK"
     gene            complement(1329..2033)
                     /locus_tag="ACHK49_005607"
     CDS             complement(1329..2033)
                     /locus_tag="ACHK49_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFO9266011.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     gene            complement(2045..3103)
                     /gene="intI1"
                     /locus_tag="ACHK49_005608"
     CDS             complement(2045..3103)
                     /gene="intI1"
                     /locus_tag="ACHK49_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFO9266012.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SEALLQIVGGDKLIIPFC"
     gene            3260..3733
                     /gene="dfrA1"
                     /locus_tag="ACHK49_005609"
     CDS             3260..3733
                     /gene="dfrA1"
                     /locus_tag="ACHK49_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFO9266013.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
BASE COUNT          916 a          985 c         1019 g          835 t
ORIGIN      
        1 ctgccgttgc tagacattgc cagccagtgc ccgtcactcc tggccagtta tgcgttgggt
       61 acgaccgctg caaatgctcg tgccctggat agcacagaag aacgaataca agactatcgg
      121 cgtgagcagc gcatattgtc cgacaattca atcagcggcc agagcgtcca gcgcggcaca
      181 ctgccacccg ataccagacc ctaccttgca ccggattggc caacactggc tgaaaataca
      241 atgccgtctg ttattgcttt gtcagcaatc gagcagccct acggctttgc tgaaaacagt
      301 ccggcctggc ttattcctgg cgttcaacaa aacgctgcca cagcagcatt gacgcactac
      361 cgcgcctggc aaaaaggctg ggcaaaacaa gacgcaaagc gacagcatca aactatatgc
      421 ggcggtttcg tcatcgaagg caaccgagtg ctaacagcgg cgcattgtgt ggacaggcaa
      481 gaagccttgc gaatccgtac agcgggcggt gaatggcggg cggctcatgt tgtcggtgct
      541 gatgtaacgc aggacgttgc tttgcttgag tttgaaggcg acccattgcc accgattaag
      601 gtggccaaca ctctacccag gccgggccag gacgttatgg ccataggctc gccgtccggt
      661 tacggctttg ctgtcggtgt cggcattgtt gcctggcacg gcctcgatgc aaagatgcta
      721 tcccctaatg atttcatgct tgtaacggct tcagtcgtgg gcggtaattc cggcggcgtg
      781 gtcgtgaata cgcacggtga agcggtttca ttggttagct atggcagcgg cagttatacg
      841 caaacagtgc cgattgaccg agctttaacc gttgctggcc agctggccaa gtaacgctac
      901 cccaaggccg ggcaaatttt gcccaggtca attaccaagc gccgaagggc ggcgcatgaa
      961 gaaaaaaaac attaaaaaac tggagtaaat cactatgaaa attcgcaatg tattttgttc
     1021 actggttgtt gcctttgctg ttgtgtctgt ttcacaggca gcgactgcca ctgatttgga
     1081 cgttgtgcat actgcgtatg aagcctacga aggagctgca aaggctttag agcgcagcat
     1141 tgacgttgtg catagcaaac tgaacaccgc aggtttaacg ccagaagaaa tgcaaaagct
     1201 gcaactacag cttgatgctt tgaaggcaca gcatgacgtg gcactgggcc gggtgatggt
     1261 tgaagctgca aagaaaggca ctgttgcaaa gttagcgatg aggcagcctt ttgtcttatt
     1321 caaaggcctt acatttcaaa aactctgctt accaggcgca tttcgcccag gggatcacca
     1381 taataaaatg ctgaggcctg gcctttgcgt agtgcacgca tcacctcaat acctttgatg
     1441 gtggcgtaag ccgtcttcat ggatttaaat cccagcgtgg cgttgattat ccgtttcagt
     1501 ttgccatgat cgcattcaat cacgttgttc cggtacttaa tctgtcggtg ttcaacgtca
     1561 gacgggcacc ggccttcgcg tttgagcaga gcaagcgcgc gaccataggc gggcgcttta
     1621 tccgtgttga tgaatcgtgg gatctgccac ttcttcacgt tgttgaggat tttacccaga
     1681 aaccggtatg cagctttgct gttacgacgg gaggagagat aaaaatcgac agtgcggccc
     1741 cggctgtcga cggcccggta cagatacgcc cagcggccat tgaccttcac gtaggtttca
     1801 tccatgtgcc acgggcaaag atcggaaggg ttacgccagt accagcgcag ccgtttttcc
     1861 atttcaggcg cataacgctg aacccagcgg taaatcgtgg agtgatcgac attcactccg
     1921 cgttcagcca gcatctcctg cagctcacgg taactgatgc cgtatttgca gtaccagcgt
     1981 acggcccaca gaatgatgtc acgctgaaaa tgccggcctt tgaatgggtt catgtgcagc
     2041 tccatcagca aaaggggatg ataagtttat caccaccgac tatttgcaac agtgcctcac
     2101 tagtgagggg cggcagcgca tcaagcggtg agcgcactcc ggcaccgcca actttcagca
     2161 catgcgtgta aatcatcgtc gtagagacgt cggaatggcc gagcagatcc tgcacggttc
     2221 gaatgtcgta accgctgcgg agcaaggccg tcgcgaacga gtggcggagg gtgtgcggtg
     2281 tggcgggctt cgtgatgcct gcttgttcta cggcacgttt gaaggcgcgc tgaaaggtct
     2341 ggtcatacat gtgatggcga cgcacgacac cgctccgtgg atcggtcgaa tgcgtgtgct
     2401 gcgcaaaaac ccagaaccac ggccaggaat gcccggcgcg cggatacttc cgctcaaggg
     2461 cgtcgggaag cgcaacgccg ctgcggccct cggcctggtc cttcagccac catgcccgtg
     2521 cacgcgacag ctgctcgcgc aggctgggtg ccaagctctc gggtaacatc aaggcccgat
     2581 ccttggagcc cttgccctcc cgcacgatga tcgtgccgtg atcgaaatcc agatccttga
     2641 cccgcagttg caaaccctca ctgatccgca tgcccgttcc atacagaagc tgggcgaaca
     2701 aacgatgctc gccttccaga aaaccgagga tgcgaaccac ttcatccggg gtcagcacca
     2761 ccggcaagcg ccgcgacggc cgaggtcttc cgatctcctg aagccagggc agatccgtgc
     2821 acagcacctt gccgtagaag aacagcaagg ccgccaatgc ctgacgatgc gtggagaccg
     2881 aaaccttgcg ctcgttcgcc agccaggaca gaaatgcctc gacttcgctg ctgcccaagg
     2941 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     3001 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     3061 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     3121 tttttgtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     3181 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     3241 taacctctga ggaagaattg tgaaactatc actaatggta gctatatcga agaatggagt
     3301 tatcgggaat ggccctgata ttccatggag tgccaaaggt gaacagctcc tgtttaaagc
     3361 tattacctat aaccaatggc tgttggttgg acgcaagact tttgaatcaa tgggagcatt
     3421 acccaaccga aagtatgcgg tcgtaacacg ttcaagtttt acatctgaca atgagaacgt
     3481 attgatcttt ccatcaatta aagatgcttt aaccaaccta aagaaaataa cggatcatgt
     3541 cattgtttca ggtggtgggg agatatacaa aagcctgatc gatcaagtag atacactaca
     3601 tatatctaca atagacatcg agccggaagg tgatgtttac tttcctgaaa tccccagcaa
     3661 ttttaggcca gtttttaccc aagacttcgc ctctaacata aattatagtt accaaatctg
     3721 gcaaaagggt taacaagtgg cagcaacgga ttcgc
//
LOCUS       DAZGLJ010000745         3478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.guided.322, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000745 DAZGLJ010000000
VERSION     DAZGLJ010000745.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.guided.322"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            44..880
                     /locus_tag="ACHK49_005610"
     CDS             44..880
                     /locus_tag="ACHK49_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFO9266014.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            1048..1314
                     /locus_tag="ACHK49_005611"
     CDS             1048..1314
                     /locus_tag="ACHK49_005611"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9266015.1"
                     /translation="MASPPGNWKTRYKASDLADHQRLEMTCKKCNRLVYINKAMISSI
                     RGHEQLYLDEVERRARCKAQNCRGTMRMAMVRLDELSGFIGGIA"
     gene            complement(1330..2505)
                     /gene="tet(Y)"
                     /locus_tag="ACHK49_005612"
     CDS             complement(1330..2505)
                     /gene="tet(Y)"
                     /locus_tag="ACHK49_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(Y)"
                     /protein_id="HFO9266016.1"
                     /translation="MSKSLITALIVVALDAIGLGLIMPVVPALLNEFVPAEQTAFHYG
                     VFLSLYAFMQVFCAPVLGRLSDRYGRRIILLVSFLGATIDYSIMAAAPVLWVLYIGRI
                     ISGVTGATGAIAASIIADTTKQEERARWFGFMGACFGAGMIAGPAIGGVLGDISVHAP
                     FVAGALLNAIAFCLVAFLLPKTPSQPPEGQPAKINLFEGFRFNFAVQGLASFFALFFL
                     MQLIGQAPAALWVIYGEQRLNWDIGTAGVSLAVFGAAHTFVQAVLTGTLSKRLGDRGV
                     LLLGMGADMCGFLLLAFITQSWMVLPAIFMLATGGIGMPALQAIISGLVCDEKQGALQ
                     GTLTGLTNITSIIGPVGFTTLYGLTAGQWDGWVWLVAASLYLIAIPLLRQSASLLRS"
     gene            2588..3211
                     /gene="tetR(Y)"
                     /locus_tag="ACHK49_005613"
     CDS             2588..3211
                     /gene="tetR(Y)"
                     /locus_tag="ACHK49_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(Y)"
                     /protein_id="HFO9266017.1"
                     /translation="MTKLDRETVIQAALELLNEVGVDNLTTRKLAERLKVQQPALYWH
                     FRNKRALLDALSEAMLEKNHTRTVPQTGEDWRVFLKENALSFRSALLSYRDGARIHAG
                     TRPTSAQYERVEKQIRFLCESGFEQPDAVRALVIVSHYTTGSVSEQQAALEDSSERKQ
                     ASKEAPAQPSQFLSHAFDTFDAEGADFAFEYGLDALISGLEMKKATK"
BASE COUNT          982 a          901 c          813 g          782 t
ORIGIN      
        1 gccttgcctt ctatctgcga ttggaccctc tgacttgggg ttgatgttca tgccgcctgt
       61 ttttcctgct cattggcacg tttcgcaacc tgttctcatt gcggacacct tttccagcct
      121 cgtttggaaa gtttcattgc cagacgggac tcctgcaatc gtcaagggat tgaaacctat
      181 agaagacatt gctgatgaac tgcgcggggc cgactatctg gtatggcgca atgggagggg
      241 agcagtccgg ttgctcggtc gtgagaacaa tctgatgttg ctcgaatatg ccggggagcg
      301 aatgctctct cacatcgttg ccgagcacgg cgactaccag gcgaccgaaa ttgcagcgga
      361 actaatggcg aagctgtatg ccgcatctga ggaacccctg ccttctgccc ttctcccgat
      421 ccgggatcgc tttgcagctt tgtttcagcg ggcgcgcgat gatcaaaacg caggttgtca
      481 aactgactac gtccacgcgg cgattatagc cgatcaaatg atgagcaatg cctcggaact
      541 gcgtgggcta catggcgatc tgcatcatga aaacatcatg ttctccagtc gcggctggct
      601 ggtgatagat cccgtcggtc tggtcggtga agtgggcttt ggcgccgcca atatgttcta
      661 cgatccggct gacagagacg acctttgtct cgatcctaga cgcattgcac agatggcgga
      721 cgcattctct cgtgcgctgg acgtcgatcc gcgtcgcctg ctcgaccagg cgtacgctta
      781 tgggtgcctt tccgcagctt ggaacgcgga tggagaagag gagcaacgcg atctagctat
      841 cgcggccgcg atcaagcagg tgcgacagac gtcatactag atatcaagcg acttctccta
      901 tcccctggga acacatcaat cttaccggag aatatcgttg gccaaagcct tagcgtagga
      961 tttcgccctc tcccgcaaac gaccccatgt gggcggtaaa atcagctatg tccgtattcc
     1021 ctcacctgaa gacttctggt gatgaaaatg gcatccccac ccggaaactg gaaaacccgc
     1081 tataaagcca gtgatcttgc agaccatcaa agattagaaa tgacctgcaa gaaatgtaac
     1141 cgccttgttt atatcaataa ggcaatgatc agctcaattc gaggacacga gcaattatat
     1201 ttggacgaag ttgaacgacg cgcacgctgc aaagctcaaa attgccgtgg caccatgcgc
     1261 atggcaatgg tgcgtcttga tgaactcagc ggttttattg gcgggattgc ttgatctccc
     1321 gccaagattt taagatcgca ataaactggc tgactggcgc aataatggta tagcaataag
     1381 ataaaggctt gctgcgacga gccaaaccca accatcccac tgccccgcgg ttaagccata
     1441 aagcgtcgta aatccgaccg gcccgataat cgaggttata ttcgtcaacc ccgttaaagt
     1501 tccctgtaaa gcaccttgtt tttcatcgca aacaagacct gaaataatgg cctgcaaagc
     1561 aggcatacca atgccgcctg tggccagcat aaaaattgcc ggcagaacca tccagctttg
     1621 cgtgataaaa gccagcaata aaaacccgca catatcagcg cccattccaa gcagcaacac
     1681 accgcggtca cccagtcgct ttgaaagagt gccggttaaa acagcttgta cgaatgtatg
     1741 tgctgcacca aaaacggcca gcgacacacc tgctgtgcca atatcccaat tcaagcgctg
     1801 ttcgccataa atcacccaca aagcggcggg cgcctgccca atcagctgca taagaaaaaa
     1861 caacgcaaaa aagctggcaa gtccctgaac tgcaaaattg aagcgaaagc cttcaaacaa
     1921 attgattttg gctggctgtc cttcaggcgg ttgtgacggc gttttgggca acaagaaagc
     1981 caccaaacaa aaggcaattg cattgagaag agcccctgcc acaaagggcg catgcacaga
     2041 tatatcacca agaacaccgc caatagcagg ccctgcaatc atacctgcac caaaacacgc
     2101 ccccataaaa ccaaaccaac gcgcacgttc ttcctgttta gttgtatcag cgataattga
     2161 tgccgcgatt gcaccagttg ctccggtaac acctgagata atccggccga tataaagcac
     2221 ccataaaaca ggcgctgccg ccattatgct ataatcaatc gtggcaccta aaaatgaaac
     2281 cagcaaaata atccgccgtc catagcggtc agataaccgc cctaaaacgg gcgcgcaaaa
     2341 gacctgcata aacgcataaa gcgataaaaa aacaccatag tgaaatgctg tttgctctgc
     2401 cggtacaaat tcatttaata aagccggaac caccggcatg attaatccca aaccaatcgc
     2461 atcaagcgcg acaacaatga gtgcggttat aagtgatttt gacatagatg tgacttttat
     2521 ttatcattga taagttggcg ataataaact ttatcaacga taaattgtca agaggtaatg
     2581 caataaaatg accaaattgg atcgtgagac cgttattcag gctgcacttg aacttctcaa
     2641 cgaagtcggt gttgacaatt taacaacgcg aaaattggcc gagcgcctga aggtgcagca
     2701 acctgctctt tattggcatt ttcgcaataa gcgcgccctg cttgatgcct tatctgaagc
     2761 catgttagaa aaaaatcaca cccgcaccgt gcctcagaca ggagaagact ggcgtgtctt
     2821 tttaaaagaa aatgcactca gctttcgtag cgcactgctt tcttatcgtg atggcgcacg
     2881 cattcatgcg ggcacacgcc caaccagtgc gcaatatgag cgcgtggaaa aacagatcag
     2941 atttttatgt gagagcggat ttgaacagcc cgatgccgta cgggcattag tgattgtcag
     3001 tcattatacg acgggctctg tcagtgagca gcaagcagcc ctcgaagata gttctgaacg
     3061 caaacaagcc tcaaaagaag cccctgcaca gccatcacag tttctatctc atgcctttga
     3121 tacattcgat gcggaaggtg ccgattttgc ctttgaatat ggattggatg ctttaatttc
     3181 cggtctggaa atgaagaaag ccacaaaata acttagcaga tgcaacataa aaaagccccg
     3241 caaagcgagg cttttaaaat gattaactta aagattatgc ttttggtgca gcttttggtt
     3301 tgcgtgttga atagacgaca aagaaccctg caaccaaacc ataaccaagc catttaaatg
     3361 gcagtatatc cgcatcttcg ctgcggattg cacgtaccgc aaacgacccc aaacaggaaa
     3421 cgcagctgaa acgggaagct caacacccac tgacgcatgg gttgttcagg cagtactt
//
LOCUS       DAZGLJ010000746         3478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.guided.323, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000746 DAZGLJ010000000
VERSION     DAZGLJ010000746.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.guided.323"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            44..880
                     /locus_tag="ACHK49_005614"
     CDS             44..880
                     /locus_tag="ACHK49_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HFO9266018.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPLRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            1048..1314
                     /locus_tag="ACHK49_005615"
     CDS             1048..1314
                     /locus_tag="ACHK49_005615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9266019.1"
                     /translation="MASPPGNWKTRYKASDLADHQRLEMTCKKCNRLVYINKAMISSI
                     RGHEQLYLDEVERRARCKAQNCRGTMRMAMVRLDELSGFIGGIA"
     gene            complement(1330..2505)
                     /gene="tet(Y)"
                     /locus_tag="ACHK49_005616"
     CDS             complement(1330..2505)
                     /gene="tet(Y)"
                     /locus_tag="ACHK49_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(Y)"
                     /protein_id="HFO9266020.1"
                     /translation="MSKSLITALIVVALDAIGLGLIMPVVPALLNEFVPAEQTAFHYG
                     VFLSLYAFMQVFCAPVLGRLSDRYGRRIILLVSFLGATIDYSIMAAAPVLWVLYIGRI
                     ISGVTGATGAIAASIIADTTKQEERARWFGFMGACFGAGMIAGPAIGGVLGDISVHAP
                     FVAGALLNAIAFCLVAFLLPKTPSQPPEGQPAKINLFEGFRFNFAVQGLASFFALFFL
                     MQLIGQAPAALWVIYGEQRLNWDIGTAGVSLAVFGAAHTFVQAVLTGTLSKRLGDRGV
                     LLLGMGADMCGFLLLAFITQSWMVLPAIFMLATGGIGMPALQAIISGLVCDEKQGALQ
                     GTLTGLTNITSIIGPVGFTTLYGLTAGQWDGWVWLVAASLYLIAIPLLRQSASLLRS"
     gene            2588..3211
                     /gene="tetR(Y)"
                     /locus_tag="ACHK49_005617"
     CDS             2588..3211
                     /gene="tetR(Y)"
                     /locus_tag="ACHK49_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011931118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(Y)"
                     /protein_id="HFO9266021.1"
                     /translation="MTKLDRETVIQAALELLNEVGVDNLTTRKLAERLKVQQPALYWH
                     FRNKRALLDALSEAMLEKNHTRTVPQTGEDWRVFLKENALSFRSALLSYRDGARIHAG
                     TRPTSAQYERVEKQIRFLCESGFEQPDAVRALVIVSHYTTGSVSEQQAALEDSSERKQ
                     ASKEAPAQPSQFLSHAFDTFDAEGADFAFEYGLDALISGLEMKKATK"
BASE COUNT          981 a          902 c          813 g          782 t
ORIGIN      
        1 gccttgcctt ctatctgcga ttggaccctc tgacttgggg ttgatgttca tgccgcctgt
       61 ttttcctgct cattggcacg tttcgcaacc tgttctcatt gcggacacct tttccagcct
      121 cgtttggaaa gtttcattgc cagacgggac tcctgcaatc gtcaagggat tgaaacctat
      181 agaagacatt gctgatgaac tgcgcggggc cgactatctg gtatggcgca atgggagggg
      241 agcagtccgg ttgctcggtc gtgagaacaa tctgatgttg ctcgaatatg ccggggagcg
      301 aatgctctct cacatcgttg ccgagcacgg cgactaccag gcgaccgaaa ttgcagcgga
      361 actaatggcg aagctgtatg ccgcatctga ggaacccctg ccttctgccc ttctcccgct
      421 ccgggatcgc tttgcagctt tgtttcagcg ggcgcgcgat gatcaaaacg caggttgtca
      481 aactgactac gtccacgcgg cgattatagc cgatcaaatg atgagcaatg cctcggaact
      541 gcgtgggcta catggcgatc tgcatcatga aaacatcatg ttctccagtc gcggctggct
      601 ggtgatagat cccgtcggtc tggtcggtga agtgggcttt ggcgccgcca atatgttcta
      661 cgatccggct gacagagacg acctttgtct cgatcctaga cgcattgcac agatggcgga
      721 cgcattctct cgtgcgctgg acgtcgatcc gcgtcgcctg ctcgaccagg cgtacgctta
      781 tgggtgcctt tccgcagctt ggaacgcgga tggagaagag gagcaacgcg atctagctat
      841 cgcggccgcg atcaagcagg tgcgacagac gtcatactag atatcaagcg acttctccta
      901 tcccctggga acacatcaat cttaccggag aatatcgttg gccaaagcct tagcgtagga
      961 tttcgccctc tcccgcaaac gaccccatgt gggcggtaaa atcagctatg tccgtattcc
     1021 ctcacctgaa gacttctggt gatgaaaatg gcatccccac ccggaaactg gaaaacccgc
     1081 tataaagcca gtgatcttgc agaccatcaa agattagaaa tgacctgcaa gaaatgtaac
     1141 cgccttgttt atatcaataa ggcaatgatc agctcaattc gaggacacga gcaattatat
     1201 ttggacgaag ttgaacgacg cgcacgctgc aaagctcaaa attgccgtgg caccatgcgc
     1261 atggcaatgg tgcgtcttga tgaactcagc ggttttattg gcgggattgc ttgatctccc
     1321 gccaagattt taagatcgca ataaactggc tgactggcgc aataatggta tagcaataag
     1381 ataaaggctt gctgcgacga gccaaaccca accatcccac tgccccgcgg ttaagccata
     1441 aagcgtcgta aatccgaccg gcccgataat cgaggttata ttcgtcaacc ccgttaaagt
     1501 tccctgtaaa gcaccttgtt tttcatcgca aacaagacct gaaataatgg cctgcaaagc
     1561 aggcatacca atgccgcctg tggccagcat aaaaattgcc ggcagaacca tccagctttg
     1621 cgtgataaaa gccagcaata aaaacccgca catatcagcg cccattccaa gcagcaacac
     1681 accgcggtca cccagtcgct ttgaaagagt gccggttaaa acagcttgta cgaatgtatg
     1741 tgctgcacca aaaacggcca gcgacacacc tgctgtgcca atatcccaat tcaagcgctg
     1801 ttcgccataa atcacccaca aagcggcggg cgcctgccca atcagctgca taagaaaaaa
     1861 caacgcaaaa aagctggcaa gtccctgaac tgcaaaattg aagcgaaagc cttcaaacaa
     1921 attgattttg gctggctgtc cttcaggcgg ttgtgacggc gttttgggca acaagaaagc
     1981 caccaaacaa aaggcaattg cattgagaag agcccctgcc acaaagggcg catgcacaga
     2041 tatatcacca agaacaccgc caatagcagg ccctgcaatc atacctgcac caaaacacgc
     2101 ccccataaaa ccaaaccaac gcgcacgttc ttcctgttta gttgtatcag cgataattga
     2161 tgccgcgatt gcaccagttg ctccggtaac acctgagata atccggccga tataaagcac
     2221 ccataaaaca ggcgctgccg ccattatgct ataatcaatc gtggcaccta aaaatgaaac
     2281 cagcaaaata atccgccgtc catagcggtc agataaccgc cctaaaacgg gcgcgcaaaa
     2341 gacctgcata aacgcataaa gcgataaaaa aacaccatag tgaaatgctg tttgctctgc
     2401 cggtacaaat tcatttaata aagccggaac caccggcatg attaatccca aaccaatcgc
     2461 atcaagcgcg acaacaatga gtgcggttat aagtgatttt gacatagatg tgacttttat
     2521 ttatcattga taagttggcg ataataaact ttatcaacga taaattgtca agaggtaatg
     2581 caataaaatg accaaattgg atcgtgagac cgttattcag gctgcacttg aacttctcaa
     2641 cgaagtcggt gttgacaatt taacaacgcg aaaattggcc gagcgcctga aggtgcagca
     2701 acctgctctt tattggcatt ttcgcaataa gcgcgccctg cttgatgcct tatctgaagc
     2761 catgttagaa aaaaatcaca cccgcaccgt gcctcagaca ggagaagact ggcgtgtctt
     2821 tttaaaagaa aatgcactca gctttcgtag cgcactgctt tcttatcgtg atggcgcacg
     2881 cattcatgcg ggcacacgcc caaccagtgc gcaatatgag cgcgtggaaa aacagatcag
     2941 atttttatgt gagagcggat ttgaacagcc cgatgccgta cgggcattag tgattgtcag
     3001 tcattatacg acgggctctg tcagtgagca gcaagcagcc ctcgaagata gttctgaacg
     3061 caaacaagcc tcaaaagaag cccctgcaca gccatcacag tttctatctc atgcctttga
     3121 tacattcgat gcggaaggtg ccgattttgc ctttgaatat ggattggatg ctttaatttc
     3181 cggtctggaa atgaagaaag ccacaaaata acttagcaga tgcaacataa aaaagccccg
     3241 caaagcgagg cttttaaaat gattaactta aagattatgc ttttggtgca gcttttggtt
     3301 tgcgtgttga atagacgaca aagaaccctg caaccaaacc ataaccaagc catttaaatg
     3361 gcagtatatc cgcatcttcg ctgcggattg cacgtaccgc aaacgacccc aaacaggaaa
     3421 cgcagctgaa acgggaagct caacacccac tgacgcatgg gttgttcagg cagtactt
//
LOCUS       DAZGLJ010000747         2558 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.guided.363, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000747 DAZGLJ010000000
VERSION     DAZGLJ010000747.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.guided.363"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            complement(<1..1182)
                     /gene="pepD"
                     /locus_tag="ACHK49_005618"
     CDS             complement(<1..1182)
                     /gene="pepD"
                     /locus_tag="ACHK49_005618"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HFO9266022.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KG"
     gene            1443..1901
                     /gene="gpt"
                     /locus_tag="ACHK49_005619"
     CDS             1443..1901
                     /gene="gpt"
                     /locus_tag="ACHK49_005619"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO9266023.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1993..>2558
                     /locus_tag="ACHK49_005620"
     CDS             1993..>2558
                     /locus_tag="ACHK49_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9266024.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMP"
BASE COUNT          607 a          672 c          647 g          632 t
ORIGIN      
        1 gcctttcgct tcggttttcg cgccagctag tttacccagc gaatccagca tgctcaccac
       61 gtagtcttta ccgctgtcga tcagtgaacg gatcaggcag tgaatttcta cgttattgtc
      121 agtcatggtc accacaccga cgttcaggga ggtttcgacc acaccttttg ccacgtcgga
      181 attgcggatc acaccgttcg gggtggcgtt cagcagacga ataaaggtat cgcgagattt
      241 cgcaatcagg gcagctttat cgttcgctac agagtccagc aacaaggcca gatttttctc
      301 tttctctgcc agctcgtttt tcaggatctc ctgataggta ttcaccagag atttcaggac
      361 gtcgacttta tcagctgcga cagcaatggt cgcaaaggct tcacgcggga tggcgttacg
      421 cagtgtgccg ccgttgaaat cgataaggcg cagatccagt tcttccgcat gacccgccag
      481 gaagcgcacc agtagtttgt tggcattacc caggccaacg tggatttcac cgccggagtg
      541 accgcctttc agacctttta aggttaactt gaaggtttca aaaccagctg gaaccgcttc
      601 acgatctaaa tgcaggttgg aggtgaagtc gatacccccc gcacaaccca tgtagatttc
      661 accttcttct tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt tgctctgtaa
      721 gccgaacgca ccgtccatac cggcttcttc ggtcatggtc agcagcactt ccagcgggcc
      781 gtgaaccacg ttttcgtcag ccagaaccgc cagcgcagac gccataccaa tgccgttatc
      841 tgcacccagc gtggtgccgc gcgctttaac ccattcgcca tcaatataag gctggatagg
      901 atctttcgtg aagtcatgca cggtgtcgtt atttttctgc ggcaccatat cgaggtgggc
      961 ctgtaagacg accggtttac gattttccat acctgcggta gcaggtttac gaatcaggat
     1021 attacctacc tgatcgcgtt cgacatggaa acctttctct tttgcccaac caacaatgta
     1081 ttcagcgagt tgctcttcat gataggacgg gtgaggaata gaacagattt tggcaaaaat
     1141 atcccacagc ggctgtggag ataattgaga cagttcagac acgttaagtc tccttgtcga
     1201 tcacccgcaa aacagtattg caggtcacag ggttagcaga aaatgttgtc aacacaagac
     1261 aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
     1321 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg
     1381 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
     1441 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
     1501 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
     1561 tggtaccggg tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta
     1621 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg
     1681 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gctgttgcga
     1741 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc
     1801 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggatcgaa cagccgtggg
     1861 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac
     1921 tgccgggcgt tgttcttttt aacttcaggc aggttacaat agtttccagt aagtattctg
     1981 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa
     2041 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag
     2101 tcggctcttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg
     2161 atctggcgcg gtattgaccc acgtgaaatc ctcgacgtcc aggcacgtat tgtgatgagc
     2221 gatgctgaac gtaccgacga tgatttatac gatactgtga ttggctaccg tggtggcaac
     2281 tggatttatg agtgggccac ccaggcgatg gtgtggcaac aaaaagcctg tgcggaagaa
     2341 gatccgcaac tcagtggtcg acactggctg catgcggcta cgttgtacaa cattgccgcc
     2401 tatcctcatc tgaaaggaga tgacctggcc gagcaagcgc aggctttatc aaaccgcgcc
     2461 tatgaagagg ccgcccagcg tctgccgggc acgatgcggc agatggagtt taccgtaccc
     2521 ggcggtgcgc ccatcaccgg ctttttgcat atgccgaa
//
LOCUS       DAZGLJ010000748         2547 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.guided.364, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000748 DAZGLJ010000000
VERSION     DAZGLJ010000748.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.guided.364"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..625
                     /locus_tag="ACHK49_005621"
     CDS             <1..625
                     /locus_tag="ACHK49_005621"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9266025.1"
                     /translation="FLLRTTVLAGEVIIQENNRMLKRRDAFLKKSALAVSVALLLSAQ
                     AQAVLTGPVDANSSSLLIGENSFITNSTGTANNTFLLGGGAFNMDSPGSLQFGSFSGV
                     YNSPHSVTLGRDAGQAESKYGVAIGKSAEVLNSQQSVAIGGWAGIENSSGSVALGHGS
                     QVSGENNVVSVGAGPEGYGESVKGAPETHSIVNKTFLSFIFNGLFSC"
     gene            872..1687
                     /gene="sul2"
                     /locus_tag="ACHK49_005622"
     CDS             872..1687
                     /gene="sul2"
                     /locus_tag="ACHK49_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFO9266026.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1748..>2547
                     /locus_tag="ACHK49_005623"
                     /note="aph(3'')-Ib"
     CDS             1748..>2547
                     /locus_tag="ACHK49_005623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFO9266027.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT          535 a          657 c          712 g          643 t
ORIGIN      
        1 atttttgctc cgcactaccg ttctggctgg tgaagtaata atacaggaga ataacagaat
       61 gcttaaaaga cgtgatgctt ttttaaagaa aagcgcgctg gcagtgagtg tggccctgct
      121 gctctcggct caggctcagg ctgttttgac agggcctgtt gatgctaatt cttccagctt
      181 actgattgga gaaaactctt ttattacgaa ttcaactggt acagctaata acactttcct
      241 tttgggaggt ggcgctttta acatggattc accaggaagt cttcagtttg gctctttttc
      301 gggagtgtat aattctccac acagtgtcac cctgggaagg gatgccggtc aggctgagtc
      361 aaaatatggt gttgccatag gtaagagcgc tgaggtactt aattcacagc agagtgttgc
      421 tattggggga tgggctggaa tagaaaattc ttcaggctct gtagctctgg gccatggctc
      481 acaggtgtcg ggagaaaata acgtggtatc tgttggtgcc gggcctgagg gatatggaga
      541 gagtgtcaaa ggagcaccgg aaacccactc tattgtaaac aagacatttt tatcttttat
      601 attcaatggc ttattttcct gctaattggt aataccatga aaaataccat gctcagaaaa
      661 ggcttaacaa tattttgaaa aattgcctac tgagcgctgc cgcacagctc cataggccgc
      721 tttcctggct ttgcttccag atgtatgcta ttctgctcct gcagctaatg gatcaccgca
      781 aacaggttac tcgcctgggg attccctttc gacccgagca tccgtatgag actcatgctc
      841 gattattatt attattatta tagaagcccc catgaataaa tcgctcatca ttttcggcat
      901 cgtcaacata acctcggaca gtttctccga tggaggccgg tatctggcgc cagacgcagc
      961 cattgcgcag gcgcgtaagc tgatggccga gggggcagat gtgatcgacc tcggtccggc
     1021 atccagcaat cccgacgccg cgcctgtttc gtccgacaca gaaatcgcgc gtatcgcgcc
     1081 ggtgctggac gcgctcaagg cagatggcat tcccgtctcg ctcgacagtt atcaacccgc
     1141 gacgcaagcc tatgccttgt cgcgtggtgt ggcctatctc aatgatattc gcggttttcc
     1201 agacgctgcg ttctatccgc aattggcgaa atcatctgcc aaactcgtcg ttatgcattc
     1261 ggtgcaagac gggcaggcag atcggcgcga ggcacccgct ggcgacatca tggatcacat
     1321 tgcggcgttc tttgacgcgc gcatcgcggc gctgacgggt gccggtatca aacgcaaccg
     1381 ccttgtcctt gatcccggca tggggttttt tctgggggct gctcccgaaa cctcgctctc
     1441 ggtgctggcg cggttcgatg aattgcggct gcgcttcgat ttgccggtgc ttctgtctgt
     1501 ttcgcgcaaa tcctttctgc gcgcgctcac aggccgtggt ccgggggatg tcggggccgc
     1561 gacactcgct gcagagcttg ccgccgccgc aggtggagct gacttcatcc gcacacacga
     1621 gccgcgcccc ttgcgcgacg ggctggcggt attggcggcg ctgaaagaaa ccgcaagaat
     1681 tcgttaactg cacattcggg atatttctct atattcgcgc ttcatcagaa aactgaagga
     1741 acctccattg aatcgaacta atattttttt tggtgaatcg cattctgact ggttgcctgt
     1801 cagaggcgga gaatctggtg attttgtttt tcgacgtggt gacgggcatg ccttcgcgaa
     1861 aatcgcacct gcttcccgcc gcggtgagct cgctggagag cgtgaccgcc tcatttggct
     1921 caaaggtcga ggtgtggctt gccccgaggt catcaactgg caggaggaac aggagggtgc
     1981 atgcttggtg ataacggcaa ttccgggagt accggcggct gatctgtctg gagcggattt
     2041 gctcaaagcg tggccgtcaa tggggcagca acttggcgct gttcacagcc tatcggttga
     2101 tcaatgtccg tttgagcgca ggctgtcgcg aatgttcgga cgcgccgttg atgtggtgtc
     2161 ccgcaatgcc gtcaatcccg acttcttacc ggacgaggac aagagtacgc cgcagctcga
     2221 tcttttggct cgtgtcgaac gagagctacc ggtgcggctc gaccaagagc gcaccgatat
     2281 ggttgtttgc catggtgatc cctgcatgcc gaacttcatg gtggacccta aaactcttca
     2341 atgcacgggt ctgatcgacc ttgggcggct cggaacagca gatcgctatg ccgatttggc
     2401 actcatgatt gctaacgccg aagagaactg ggcagcgcca gatgaagcag agcgcgcctt
     2461 cgctgtccta ttcaatgtat tggggatcga agcccccgac cgcgaacgcc ttgccttcta
     2521 tctgcgattg gaccctctga cttgggg
//
LOCUS       DAZGLJ010000749         2469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.guided.370, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000749 DAZGLJ010000000
VERSION     DAZGLJ010000749.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.guided.370"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            109..993
                     /locus_tag="ACHK49_005624"
     CDS             109..993
                     /locus_tag="ACHK49_005624"
                     /inference="COORDINATES: protein motif:HMM:NF023271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /protein_id="HFO9266028.1"
                     /translation="MLRSQRRGRDPPCWLSEKEPLVGKRRVNFQRCWLPVSRIGLHRR
                     GVERVNCGPKLLDLGEKGVYSSYTCRVPGGDYEGYVDAHVRRLEALRRAGIVERIDAD
                     QWRIPDDLVSRAAAHDAGRDSQASVRVLSPVDLNKQIGSDGATWLDRRLIHGETADLA
                     PTGFGQQVREAMDQRREHHIEQGDATRSRDSRVFYRRNLLAILREREVAGVGSDMALS
                     KGLPFRAATDGESVSGKFTGTVHLSSGKFAVVEKSHEFTLVPWRPIIDRQLGREVMGI
                     VQGGSVSWQLGRQRGLER"
     gene            1210..2424
                     /gene="floR"
                     /locus_tag="ACHK49_005625"
     CDS             1210..2424
                     /gene="floR"
                     /locus_tag="ACHK49_005625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol/florfenicol efflux MFS
                     transporter FloR"
                     /protein_id="HFO9266029.1"
                     /translation="MTTTRPAWAYTLPAALLLMAPFDILASLAMDIYLPVVPAMPGIL
                     NTTPAMIQLTLSLYMVMLGVGQVIFGPLSDRIGRRPILLAGATAFVIASLGAAWSSTA
                     PAFVAFRLLQAVGASAMLVATFATVRDVYANRPEGVVIYGLFSSMLAFVPALGPIAGV
                     LIGEFLGWQAIFITLAILAMLALLNAGFRWHETRPLDQVKTRRSVLPIFASPAFWVYT
                     VGFSAGMGTYFVFFSTAPRVLIGQAEYSEIGFSFAFATVARVMIVTTRFAKSFVARWG
                     IAGCVARGMALLVCGAVLLGIGELYGSPSFLTFILPMWVVAVGIVFTVSVTANGALAE
                     FDDIAGSAVAFYFCVQSLIVSIVGTLAVALLNGDTAWPVICYATAMAVLVSLGLVLLR
                     LRGAATEKSPVV"
BASE COUNT          439 a          714 c          768 g          548 t
ORIGIN      
        1 gccgaaacca gcgggaccag ggcgttaccc gaaagccggg cgccaccggc tgagctgctc
       61 ctgggtctgt ttgactgacg agcctgaagg ccaacgatac caatcaaaat gctgcgttca
      121 cagcgccgcg gcagggatcc gccgtgctgg ttgtcggaaa aggagccgct agtgggaaag
      181 aggagggtaa attttcagcg ttgctggctc cccgtcagcc ggattgggtt gcatcgcagg
      241 ggtgtcgaaa gagtcaactg cggtccaaag ctgttggact tgggtgaaaa gggcgtttat
      301 tcttcctata cgtgccgcgt tccaggcggc gactatgagg gctatgtcga tgcccatgtg
      361 cgccggctgg aggcgctacg ccgggccggt atcgtcgagc ggatcgacgc cgaccaatgg
      421 cgcatccccg atgatctggt cagccgtgcc gccgcccatg acgccggccg agacagtcag
      481 gccagcgttc gcgtcctttc cccggtcgat ctgaacaaac agatcggatc ggacggcgcg
      541 acctggctgg accggcggct gatccacggc gagacggccg accttgcgcc aaccggcttc
      601 gggcaacaag tccgcgaagc catggaccag cgccgcgagc accatatcga acagggcgac
      661 gccacccgca gccgggacag ccgcgtcttc taccggcgca accttctcgc catcctgcgg
      721 gagcgcgagg tagccggcgt cggatcggat atggctttga gtaagggcct gccgttccgc
      781 gccgccacgg acggcgagag cgtcagcggc aagtttaccg gaaccgtgca tctatcgagc
      841 ggcaagttcg ccgtggtcga gaaatcccat gagttcaccc ttgtcccgtg gcggccgatc
      901 atcgaccgcc aactcggccg cgaggttatg ggcatcgtgc agggcgggtc ggtgtcgtgg
      961 cagttagggc ggcagagggg gctggaacgc tgagtgcgcc catgccgcat tgcgaagcaa
     1021 aagataatcg gataaaatgt agcaattcat attcgtaagc gtggagtaat cagatgggaa
     1081 attccaagtc agcagacaag taagccgcaa caaccagtat tgttgttgcg gcgctctgta
     1141 aggctagtct catctgattg ctgacgagca gacgtcgccc ggtattcctt aatcgagggt
     1201 tgattcgtca tgaccaccac acgccccgcg tgggcctata cgctgccggc agcactgctg
     1261 ctgatggctc ctttcgacat cctcgcttca ctggcgatgg atatttatct ccctgtcgtt
     1321 ccagcgatgc ccggcatcct gaacacgacg cccgctatga tccaactcac gttgagcctc
     1381 tatatggtga tgctcggcgt gggccaggtg atttttggtc cgctctcaga cagaatcggg
     1441 cgacggccaa ttctacttgc gggcgcaacg gctttcgtca ttgcgtctct gggagcagct
     1501 tggtcttcaa ctgcaccggc ctttgtcgct ttccgtctac ttcaagcagt gggcgcgtcg
     1561 gccatgctgg tggcgacgtt cgcgacggtt cgcgacgttt atgccaaccg tcctgagggt
     1621 gtcgtcatct acggcctttt cagttcgatg ctggcgttcg tgcctgcgct cggccctatc
     1681 gccggagtat tgatcggcga gttcttggga tggcaggcga tattcattac tttggctata
     1741 ctggcgatgc tcgcactcct aaatgcgggt ttcaggtggc acgaaacccg ccctctggat
     1801 caagtcaaga cgcgccgatc tgtcttgccg atcttcgcga gtccggcttt ttgggtttac
     1861 actgtcggct ttagcgccgg tatgggcacc tacttcgtct tcttctcgac ggctccccgt
     1921 gtgctcatag gccaagcgga atattccgag atcggattca gctttgcctt cgccactgtc
     1981 gcgcgtgtaa tgatcgtgac aacccgtttc gcgaagtcct ttgtcgccag atggggcatc
     2041 gcaggatgcg tggcgcgtgg gatggcgttg cttgtttgcg gagcggtcct gttggggatc
     2101 ggcgaacttt acggctcgcc gtcattcctc accttcatcc taccgatgtg ggttgtcgcg
     2161 gtcggtattg tcttcacggt gtccgttacc gcgaacggcg ctttggcaga gttcgacgac
     2221 atcgcgggat cagcggtcgc gttctacttc tgcgttcaaa gcctgatagt cagcattgtc
     2281 gggacattgg cggtggcact tttaaacggt gacacagcgt ggcccgtgat ctgttacgcc
     2341 acggcgatgg cggtactggt ttcgttgggg ctggtgctcc ttcggctccg tggggctgcc
     2401 accgagaagt cgccagtcgt ctaaccgaag actggtagca ggcccgctcc gatgcggcgc
     2461 actaaccat
//
LOCUS       DAZGLJ010000750         2469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.guided.371, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000750 DAZGLJ010000000
VERSION     DAZGLJ010000750.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.guided.371"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            109..993
                     /locus_tag="ACHK49_005626"
     CDS             109..993
                     /locus_tag="ACHK49_005626"
                     /inference="COORDINATES: protein motif:HMM:NF023271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /protein_id="HFO9266030.1"
                     /translation="MLRSQRRGRDPPCWLSEKEPLVGKRRVNFQRCWLPVSRIGLHRR
                     GVERVNCGPKLLDLGEKGVYSSYTCRVPGGDYEGYVDAHVRRLEALRRAGIVERIDAD
                     QWRIPDDLVSRAAAHDAGRDSQASVRVLSPVDLNKQIGSDGATWLDRRLIHGETADLA
                     PTGFGQQVREAMDQRREHHIEQGDATRSRDSRVFYRRNLLAILREREVAGVGSDMALS
                     KGLPFRAATDGESVSGKFTGTVHLSSGKFAVVEKSHEFTLVPWRPIIDRQLGREVMGI
                     VQGGSVSWQLGRQRGLER"
     gene            1210..2424
                     /gene="floR"
                     /locus_tag="ACHK49_005627"
     CDS             1210..2424
                     /gene="floR"
                     /locus_tag="ACHK49_005627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol/florfenicol efflux MFS
                     transporter FloR"
                     /protein_id="HFO9266031.1"
                     /translation="MTTTRPAWAYTLPAALLLMAPFDILASLAMDIYLPVVPAMPGIL
                     NTTPAMIQLTLSLYMVMLGVGQVIFGPLSDRIGRRPILLAGATAFVIASLGAAWSSTA
                     PAFVAFRLLQAVGASAMLVATFATVRDVYANRPEGVVIYGLFSSMLAFVPALGPIAGV
                     LIGEFLGWQAIFITLAILAMLALLNAGFRWHETRPLDQVKTRRSVLPIFASPAFWVYT
                     VGFSAGMGTYFVFFSTAPRVLIGQAEYSEIGFSFAFATVALVMIVTTRFAKSFVARWG
                     IAGCVARGMALLVCGAVLLGIGELYGSPSFLTFILPMWVVAVGIVFTVSVTANGALAE
                     FDDIAGSAVAFYFCVQSLIVSIVGTLAVALLNGDTAWPVICYATAMAVLVSLGLVLLR
                     LRGAATEKSPVV"
BASE COUNT          439 a          714 c          767 g          549 t
ORIGIN      
        1 gccgaaacca gcgggaccag ggcgttaccc gaaagccggg cgccaccggc tgagctgctc
       61 ctgggtctgt ttgactgacg agcctgaagg ccaacgatac caatcaaaat gctgcgttca
      121 cagcgccgcg gcagggatcc gccgtgctgg ttgtcggaaa aggagccgct agtgggaaag
      181 aggagggtaa attttcagcg ttgctggctc cccgtcagcc ggattgggtt gcatcgcagg
      241 ggtgtcgaaa gagtcaactg cggtccaaag ctgttggact tgggtgaaaa gggcgtttat
      301 tcttcctata cgtgccgcgt tccaggcggc gactatgagg gctatgtcga tgcccatgtg
      361 cgccggctgg aggcgctacg ccgggccggt atcgtcgagc ggatcgacgc cgaccaatgg
      421 cgcatccccg atgatctggt cagccgtgcc gccgcccatg acgccggccg agacagtcag
      481 gccagcgttc gcgtcctttc cccggtcgat ctgaacaaac agatcggatc ggacggcgcg
      541 acctggctgg accggcggct gatccacggc gagacggccg accttgcgcc aaccggcttc
      601 gggcaacaag tccgcgaagc catggaccag cgccgcgagc accatatcga acagggcgac
      661 gccacccgca gccgggacag ccgcgtcttc taccggcgca accttctcgc catcctgcgg
      721 gagcgcgagg tagccggcgt cggatcggat atggctttga gtaagggcct gccgttccgc
      781 gccgccacgg acggcgagag cgtcagcggc aagtttaccg gaaccgtgca tctatcgagc
      841 ggcaagttcg ccgtggtcga gaaatcccat gagttcaccc ttgtcccgtg gcggccgatc
      901 atcgaccgcc aactcggccg cgaggttatg ggcatcgtgc agggcgggtc ggtgtcgtgg
      961 cagttagggc ggcagagggg gctggaacgc tgagtgcgcc catgccgcat tgcgaagcaa
     1021 aagataatcg gataaaatgt agcaattcat attcgtaagc gtggagtaat cagatgggaa
     1081 attccaagtc agcagacaag taagccgcaa caaccagtat tgttgttgcg gcgctctgta
     1141 aggctagtct catctgattg ctgacgagca gacgtcgccc ggtattcctt aatcgagggt
     1201 tgattcgtca tgaccaccac acgccccgcg tgggcctata cgctgccggc agcactgctg
     1261 ctgatggctc ctttcgacat cctcgcttca ctggcgatgg atatttatct ccctgtcgtt
     1321 ccagcgatgc ccggcatcct gaacacgacg cccgctatga tccaactcac gttgagcctc
     1381 tatatggtga tgctcggcgt gggccaggtg atttttggtc cgctctcaga cagaatcggg
     1441 cgacggccaa ttctacttgc gggcgcaacg gctttcgtca ttgcgtctct gggagcagct
     1501 tggtcttcaa ctgcaccggc ctttgtcgct ttccgtctac ttcaagcagt gggcgcgtcg
     1561 gccatgctgg tggcgacgtt cgcgacggtt cgcgacgttt atgccaaccg tcctgagggt
     1621 gtcgtcatct acggcctttt cagttcgatg ctggcgttcg tgcctgcgct cggccctatc
     1681 gccggagtat tgatcggcga gttcttggga tggcaggcga tattcattac tttggctata
     1741 ctggcgatgc tcgcactcct aaatgcgggt ttcaggtggc acgaaacccg ccctctggat
     1801 caagtcaaga cgcgccgatc tgtcttgccg atcttcgcga gtccggcttt ttgggtttac
     1861 actgtcggct ttagcgccgg tatgggcacc tacttcgtct tcttctcgac ggctccccgt
     1921 gtgctcatag gccaagcgga atattccgag atcggattca gctttgcctt cgccactgtc
     1981 gcgcttgtaa tgatcgtgac aacccgtttc gcgaagtcct ttgtcgccag atggggcatc
     2041 gcaggatgcg tggcgcgtgg gatggcgttg cttgtttgcg gagcggtcct gttggggatc
     2101 ggcgaacttt acggctcgcc gtcattcctc accttcatcc taccgatgtg ggttgtcgcg
     2161 gtcggtattg tcttcacggt gtccgttacc gcgaacggcg ctttggcaga gttcgacgac
     2221 atcgcgggat cagcggtcgc gttctacttc tgcgttcaaa gcctgatagt cagcattgtc
     2281 gggacattgg cggtggcact tttaaacggt gacacagcgt ggcccgtgat ctgttacgcc
     2341 acggcgatgg cggtactggt ttcgttgggg ctggtgctcc ttcggctccg tggggctgcc
     2401 accgagaagt cgccagtcgt ctaaccgaag actggtagca ggcccgctcc gatgcggcgc
     2461 actaaccat
//
LOCUS       DAZGLJ010000751         2088 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.guided.399, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000751 DAZGLJ010000000
VERSION     DAZGLJ010000751.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.guided.399"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            127..1086
                     /locus_tag="ACHK49_005628"
     CDS             127..1086
                     /locus_tag="ACHK49_005628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO9266032.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1098..1367
                     /locus_tag="ACHK49_005629"
     CDS             1098..1367
                     /locus_tag="ACHK49_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFO9266033.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            complement(1665..1988)
                     /locus_tag="ACHK49_005630"
     CDS             complement(1665..1988)
                     /locus_tag="ACHK49_005630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO9266034.1"
                     /translation="MNWIVIDTVIQPACDISFSAIWCNIKLILWYQSDIFLPPGCIFT
                     PTRTGVILNNKELPVTIYNVTPFNKNVWNLIKSSQECPANTGNITDKCFNHRCILEIC
                     PYGRK"
BASE COUNT          550 a          411 c          507 g          620 t
ORIGIN      
        1 aatcggtcac tggttcgaat ccagtacaac gcgccatatt tatttaccag gctcgctttt
       61 gcgggccttt tttatatctg cgccgggtct ggtgctgatt acttcagcca aaaggaacac
      121 ctgtatatga agtgtatatt atttaaatgg gtactgtgcc tgttactggg tttttcttcg
      181 gtatcctatt cccgggaatt tacgatagac ttttcgactc aacaaagtta tgtctcttcg
      241 ttaaatagta tacggacaga gatatcgacc cctcttgaac atatatctca ggggaccaca
      301 tcggtgtctg ttattaacca caccccgccg ggcagttatt ttgctgtgga tatacgaggg
      361 cttgatgtct atcaggcgcg ttttgaccat cttcgtctga ttattgagca aaataattta
      421 tatgtggccg ggttcgttaa tacggcaaca aatactttct accgtttttc agattttaca
      481 catatatcag tgcccgatgt gacaacggtt tccatgacaa cggacagcag ttataccact
      541 ctgcaacgtg tcgcagcgct ggaacgttcc ggaatgcaaa tcagtcgtca ctcactggtt
      601 tcatcatatc tggcgttaat ggagttcagt ggtaatacaa tgaccagaga tgcatccaga
      661 gcagttctgc gttttgtcac tgtcacggca gaagccttac gcttcaggca gatacagaga
      721 gaatttcgtc aggcactgtc tgaaactgct cctgtgtata cgatgacgcc gggagacgtg
      781 gacctcactc tgaactgggg gcgaatcagc aatgtgcttc cggagtatcg gggagaggat
      841 ggtgtcagag tggggagaat atcctttaat aatatatcag cgatactggg tactgtggcc
      901 gttatactga attgccatca tcagggggcg cgttctgttc gcgccgtgaa tgaagagagt
      961 caaccagaat gtcagataac tggcgacagg cccgttataa aaataaacaa tacattatgg
     1021 gaaagtaata cagctgcagc gtttctgaac agaaagtcac agtttttata tacaacgggt
     1081 aaataaagga gttaagtatg aagaagatgt ttatggcggt tttatttgca ttagtttctg
     1141 ttaatgcaat ggcggcggat tgtgctaaag gtaaaattga gttttccaag tataatgagg
     1201 atgacacatt tacagtgaag gttgacggga aagaatactg gaccagtcgc tggaatctgc
     1261 aaccgttact gcaaagtgct cagttgacag gaatgactgt cacaatcaaa tccagtacct
     1321 gtgaatcagg ctccggattt gctgaagtgc agtttaataa tgactgaggc ataacctgat
     1381 tcgtggtatg tgggtaacaa gtgtaatctg tgtcacaatt cagtcagttg acagttgcct
     1441 gtcagactga gcatttgtta aaaaaatttc gcatggtgaa gccccctgtg cggaggggcg
     1501 actggtgtat aaggtgtcat ctctgacgac aacctggatc gagatacgcg ggttcaggga
     1561 caccggactg aactcaccgg gaggcacccg gcaccatgtt catggtgata cagaaatgcg
     1621 gcttcagccc ctctccggag gggcttttta tctgaatgat tctgttattt tcgcccgtat
     1681 gggcatattt ccagaatgca gcgatgatta aaacacttat ctgtgatatt tcctgtgttt
     1741 gcagggcatt cctggctgct tttaattaaa ttccagacgt ttttattaaa tggggtcacg
     1801 ttgtaaatgg tgacggggag ctctttatta ttgagtatta cgcctgtccg ggttggtgta
     1861 aaaatacagc ccggagggag gaagatatca gactgatacc ataatattaa ttttatgtta
     1921 caccatatgg ctgaaaatga tatgtcacat gctggctgga ttaccgtatc aatcactatc
     1981 cagttcattt gctttcctta ttttcttaag gttttaaatg atgccataaa ttataagtga
     2041 tgttttgaaa gcaatctgtc agcctgagtt gtttttctgg tgtcgttt
//
LOCUS       DAZGLJ010000752         1890 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.guided.409, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000752 DAZGLJ010000000
VERSION     DAZGLJ010000752.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.guided.409"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..652
                     /gene="folP"
                     /locus_tag="ACHK49_005631"
     CDS             <1..652
                     /gene="folP"
                     /locus_tag="ACHK49_005631"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO9266035.1"
                     /translation="AAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRG
                     VAYLNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDAR
                     IAALTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSF
                     LRALTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            713..1516
                     /locus_tag="ACHK49_005632"
                     /note="aph(3'')-Ib"
     CDS             713..1516
                     /locus_tag="ACHK49_005632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFO9266036.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            1516..>1890
                     /locus_tag="ACHK49_005633"
     CDS             1516..>1890
                     /locus_tag="ACHK49_005633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HFO9266037.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLP"
BASE COUNT          355 a          528 c          557 g          450 t
ORIGIN      
        1 cgccgcgcct gtttcgtccg acacagaaat cgcgcgtatc gcgccggtgc tggacgcgct
       61 caaggcagat ggcattcccg tctcgctcga cagttatcaa cccgcgacgc aagcctatgc
      121 cttgtcgcgt ggtgtggcct atctcaatga tattcgcggt tttccagacg ctgcgttcta
      181 tccgcaattg gcgaaatcat ctgccaaact cgtcgttatg cattcggtgc aagacgggca
      241 ggcagatcgg cgcgaggcac ccgctggcga catcatggat cacattgcgg cgttctttga
      301 cgcgcgcatc gcggcgctga cgggtgccgg tatcaaacgc aaccgccttg tccttgatcc
      361 cggcatgggg ttttttctgg gggctgctcc cgaaacctcg ctctcggtgc tggcgcggtt
      421 cgatgaattg cggctgcgct tcgatttgcc ggtgcttctg tctgtttcgc gcaaatcctt
      481 tctgcgcgcg ctcacaggcc gtggtccggg ggatgtcggg gccgcgacac tcgctgcaga
      541 gcttgccgcc gccgcaggtg gagctgactt catccgcaca cacgagccgc gccccttgcg
      601 cgacgggctg gcggtattgg cggcgctgaa agaaaccgca agaattcgtt aactgcacat
      661 tcgggatatt tctctatatt cgcgcttcat cagaaaactg aaggaacctc cattgaatcg
      721 aactaatatt ttttttggtg aatcgcattc tgactggttg cctgtcagag gcggagaatc
      781 tggtgatttt gtttttcgac gtggtgacgg gcatgccttc gcgaaaatcg cacctgcttc
      841 ccgccgcggt gagctcgctg gagagcgtga ccgcctcatt tggctcaaag gtcgaggtgt
      901 ggcttgcccc gaggtcatca actggcagga ggaacaggag ggtgcatgct tggtgataac
      961 ggcaattccg ggagtaccgg cggctgatct gtctggagcg gatttgctca aagcgtggcc
     1021 gtcaatgggg cagcaacttg gcgctgttca cagcctatcg gttgatcaat gtccgtttga
     1081 gcgcaggctg tcgcgaatgt tcggacgcgc cgttgatgtg gtgtcccgca atgccgtcaa
     1141 tcccgacttc ttaccggacg aggacaagag tacgccgcag ctcgatcttt tggctcgtgt
     1201 cgaacgagag ctaccggtgc ggctcgacca agagcgcacc gatatggttg tttgccatgg
     1261 tgatccctgc atgccgaact tcatggtgga ccctaaaact cttcaatgca cgggtctgat
     1321 cgaccttggg cggctcggaa cagcagatcg ctatgccgat ttggcactca tgattgctaa
     1381 cgccgaagag aactgggcag cgccagatga agcagagcgc gccttcgctg tcctattcaa
     1441 tgtattgggg atcgaagccc ccgaccgcga acgccttgcc ttctatctgc gattggaccc
     1501 tctgacttgg ggttgatgtt catgccgcct gtttttcctg ctcattggca cgtttcgcaa
     1561 cctgttctca ttgcggacac cttttccagc ctcgtttgga aagtttcatt gccagacggg
     1621 actcctgcaa tcgtcaaggg attgaaacct atagaagaca ttgctgatga actgcgcggg
     1681 gccgactatc tggtatggcg caatgggagg ggagcagtcc ggttgctcgg tcgtgagaac
     1741 aatctgatgt tgctcgaata tgccggggag cgaatgctct ctcacatcgt tgccgagcac
     1801 ggcgactacc aggcgaccga aattgcagcg gaactaatgg cgaagctgta tgccgcatct
     1861 gaggaacccc tgccttctgc ccttctcccg
//
LOCUS       DAZGLJ010000753         1553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.guided.441, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000753 DAZGLJ010000000
VERSION     DAZGLJ010000753.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.guided.441"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            <1..652
                     /gene="folP"
                     /locus_tag="ACHK49_005634"
     CDS             <1..652
                     /gene="folP"
                     /locus_tag="ACHK49_005634"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO9266038.1"
                     /translation="AAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRG
                     VAYLNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDAR
                     IAALTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSF
                     LRALTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            713..1516
                     /locus_tag="ACHK49_005635"
                     /note="aph(3'')-Ib"
     CDS             713..1516
                     /locus_tag="ACHK49_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFO9266039.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT          282 a          437 c          463 g          371 t
ORIGIN      
        1 cgccgcgcct gtttcgtccg acacagaaat cgcgcgtatc gcgccggtgc tggacgcgct
       61 caaggcagat ggcattcccg tctcgctcga cagttatcaa cccgcgacgc aagcctatgc
      121 cttgtcgcgt ggtgtggcct atctcaatga tattcgcggt tttccagacg ctgcgttcta
      181 tccgcaattg gcgaaatcat ctgccaaact cgtcgttatg cattcggtgc aagacgggca
      241 ggcagatcgg cgcgaggcac ccgctggcga catcatggat cacattgcgg cgttctttga
      301 cgcgcgcatc gcggcgctga cgggtgccgg tatcaaacgc aaccgccttg tccttgatcc
      361 cggcatgggg ttttttctgg gggctgctcc cgaaacctcg ctctcggtgc tggcgcggtt
      421 cgatgaattg cggctgcgct tcgatttgcc ggtgcttctg tctgtttcgc gcaaatcctt
      481 tctgcgcgcg ctcacaggcc gtggtccggg ggatgtcggg gccgcgacac tcgctgcaga
      541 gcttgccgcc gccgcaggtg gagctgactt catccgcaca cacgagccgc gccccttgcg
      601 cgacgggctg gcggtattgg cggcgctgaa agaaaccgca agaattcgtt aactgcacat
      661 tcgggatatt tctctatatt cgcgcttcat cagaaaactg aaggaacctc cattgaatcg
      721 aactaatatt ttttttggtg aatcgcattc tgactggttg cctgtcagag gcggagaatc
      781 tggtgatttt gtttttcgac gtggtgacgg gcatgccttc gcgaaaatcg cacctgcttc
      841 ccgccgcggt gagctcgctg gagagcgtga ccgcctcatt tggctcaaag gtcgaggtgt
      901 ggcttgcccc gaggtcatca actggcagga ggaacaggag ggtgcatgct tggtgataac
      961 ggcaattccg ggagtaccgg cggctgatct gtctggagcg gatttgctca aagcgtggcc
     1021 gtcaatgggg cagcaacttg gcgctgttca cagcctatcg gttgatcaat gtccgtttga
     1081 gcgcaggctg tcgcgaatgt tcggacgcgc cgttgatgtg gtgtcccgca atgccgtcaa
     1141 tcccgacttc ttaccggacg aggacaagag tacgccgcag ctcgatcttt tggctcgtgt
     1201 cgaacgagag ctaccggtgc ggctcgacca agagcgcacc gatatggttg tttgccatgg
     1261 tgatccctgc atgccgaact tcatggtgga ccctaaaact cttcaatgca cgggtctgat
     1321 cgaccttggg cggctcggaa cagcagatcg ctatgccgat ttggcactca tgattgctaa
     1381 cgccgaagag aactgggcag cgccagatga agcagagcgc gccttcgctg tcctattcaa
     1441 tgtattgggg atcgaagccc ccgaccgcga acgccttgcc ttctatctgc gattggaccc
     1501 tctgacttgg gggtgatgtt catgccgcct gtttttcctg ctcattggca cgt
//
LOCUS       DAZGLJ010000754         1375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.guided.465, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000754 DAZGLJ010000000
VERSION     DAZGLJ010000754.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.guided.465"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            44..882
                     /gene="aadA1"
                     /locus_tag="ACHK49_005636"
                     /pseudo
     CDS             44..882
                     /gene="aadA1"
                     /locus_tag="ACHK49_005636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            999..1346
                     /locus_tag="ACHK49_005637"
     CDS             999..1346
                     /locus_tag="ACHK49_005637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFO9266040.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
BASE COUNT          318 a          340 c          367 g          350 t
ORIGIN      
        1 gccgcgccag gtttgcgatc cgctgtgcca ggcgttaaac atcatgaggg aagcggtgat
       61 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
      121 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
      181 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
      241 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
      301 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
      361 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
      421 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
      481 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
      541 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
      601 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
      661 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
      721 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcccgcg cagatcagtt
      781 ggaagaattt gttcactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct
      841 aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac
      901 taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag
      961 cgtgcataat aagccctaca caaattggga gatatatcat gaaaggctgg ctttttcttg
     1021 ttatcgcaat agttggcgaa gtaatcgcaa catccgcatt aaaatctagc gagggcttta
     1081 ctaagcttgc cccttccgcc gttgtcataa tcggttatgg catcgcattt tattttcttt
     1141 ctctggttct gaaatccatc cctgtcggtg ttgcttatgc agtctggtcg ggactcggcg
     1201 tcgtcataat tacagccatt gcctggttgc ttcatgggca aaagcttgat gcgtggggct
     1261 ttgtaggtat ggggctcata attgctgcct ttttgctcgc ccgatcccca tcgtggaagt
     1321 cgctgcggag gccgacgcca tggtgacggt gttcggcatt ctgaatctca ccgag
//
LOCUS       DAZGLJ010000755         1352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2105T16012
            SAMN43267488-rid23082723.guided.469, whole genome shotgun sequence.
ACCESSION   DAZGLJ010000755 DAZGLJ010000000
VERSION     DAZGLJ010000755.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267488
            Sequence Read Archive: SRR30307677
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350994.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350994.1
            Genome Coverage       :: 34x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 08:07:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,333
            CDSs (with protein)               :: 5,333
            Genes (RNA)                       :: 73
            rRNAs                             :: 1, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 98 of 232
            Pseudo Genes (incomplete)         :: 123 of 232
            Pseudo Genes (internal stop)      :: 58 of 232
            Pseudo Genes (multiple problems)  :: 42 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267488-rid23082723.guided.469"
                     /serotype="O 26:H11"
                     /isolate="2105T16012"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-05"
                     /collected_by="SSI"
                     /note="subtype: stx2a"
     gene            92..1225
                     /locus_tag="ACHK49_005638"
     CDS             92..1225
                     /locus_tag="ACHK49_005638"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO9266041.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
BASE COUNT          360 a          351 c          326 g          315 t
ORIGIN      
        1 tcacgctgat tggtatcgtt acaatctaac gtatcgccaa tgtaaatccg gcccgcctat
       61 ggcgggccgt tttgtatgga aaccagacct tatgttcaaa acgacgctct gcgccttatt
      121 aattaccgcc tcttgctcca catttgctgc ccctcaacaa atcaacgata ttgtgcatcg
      181 cacaattacc ccgcttatag agcaacaaaa gatccccggt atggcggtgg cggtaattta
      241 tcagggtaaa ccttattact ttacctgggg ctatgcggac atcgccaaaa agcagcccgt
      301 cacacagcaa acgttgtttg agttaggttc ggtcagcaaa acatttacgg gcgtgcttgg
      361 tggcgacgct attgctcgag gggaaatcaa gttaagcgat cccacaacaa aatactggcc
      421 tgaacttacc gctaaacagt ggaatgggat cacactatta catctcgcaa cctacactgc
      481 tggcggcctg ccattgcagg tgccggatga ggtgaaatcc tcaagcgact tgctgcgctt
      541 ctatcaaaac tggcagcctg catgggcgcc aggaacacaa cgtctgtatg ccaactccag
      601 tatcggtttg ttcggcgcac tggctgtgaa gccgtctggt ttgagttttg agcaggcgat
      661 gcaaacacgt gtcttccagc cactcaaact caaccatacg tggattaatg taccgcccgc
      721 agaagaaaag aattacgcct ggggatatcg cgaaggcaag gcagttcatg tttcgccagg
      781 ggcgttagat gctgaaactt atggtgtgaa gtcgaccatt gaagatatgg cctgctgggt
      841 acgaagcaat atgaatcccc gtgatatcaa cgacaaaaca cttcagcaag ggatacaact
      901 ggcacaatct cgctactggc aaaccggcga tatgtatcag ggcctgggct gggaaatgct
      961 ggactggccg gtaaatcctg acagcatcat taacggcagt ggcaataaaa ttgcactggc
     1021 agcacacccc gtaaaagcga ttacgccccc aactcctgca gtacgcgcat catgggtaca
     1081 taaaacaggg gcaaccggcg gatttggtag ctatgtcgcg tttattccag aaaaagagct
     1141 gggtatagtg atgctggcta acaaaaacta tcccaatcca gcgagagtcg ccgccgcctg
     1201 gcagattctc aacgctctac agtaaaattt catcgggtcc gaattttcgg accttttctg
     1261 cgcttttcct tgctgtcatc tacacttaga aaaaaaccag taaggaaaca tttatgcgcc
     1321 tgctccctct cgttgccgca gcgacagctg ca
//
